LOCUS       DAOTRK010000001       102215 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.001, whole genome shotgun sequence.
ACCESSION   DAOTRK010000001 DAOTRK010000000
VERSION     DAOTRK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 102215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.001"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            200..1099
                     /gene="lysO"
                     /locus_tag="QIE41_000001"
     CDS             200..1099
                     /gene="lysO"
                     /locus_tag="QIE41_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDS3528941.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITIILLCNIIALLWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1243..2895
                     /gene="hcp"
                     /locus_tag="QIE41_000002"
                     /gene_synonym="priS"
     CDS             1243..2895
                     /gene="hcp"
                     /locus_tag="QIE41_000002"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDS3528942.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2907..3875
                     /gene="hcr"
                     /locus_tag="QIE41_000003"
     CDS             2907..3875
                     /gene="hcr"
                     /locus_tag="QIE41_000003"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDS3528943.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4008..5726
                     /gene="poxB"
                     /locus_tag="QIE41_000004"
     CDS             4008..5726
                     /gene="poxB"
                     /locus_tag="QIE41_000004"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDS3528944.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATSHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5763..6764
                     /gene="ltaE"
                     /locus_tag="QIE41_000005"
     CDS             5763..6764
                     /gene="ltaE"
                     /locus_tag="QIE41_000005"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDS3528945.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6775..8205
                     /locus_tag="QIE41_000006"
     CDS             6775..8205
                     /locus_tag="QIE41_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDS3528946.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTEL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            8304..9317
                     /locus_tag="QIE41_000007"
     CDS             8304..9317
                     /locus_tag="QIE41_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDS3528947.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPSLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9314..10144)
                     /gene="amiD"
                     /locus_tag="QIE41_000008"
     CDS             complement(9314..10144)
                     /gene="amiD"
                     /locus_tag="QIE41_000008"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDS3528948.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10141..10464)
                     /locus_tag="QIE41_000009"
     CDS             complement(10141..10464)
                     /locus_tag="QIE41_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDS3528949.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10675..12477)
                     /locus_tag="QIE41_000010"
     CDS             complement(10675..12477)
                     /locus_tag="QIE41_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3528950.1"
                     /translation="MEYLFPWVIFNMINNISDQASSFPGTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILAINADSDLIKNDELLAENIPSANYQFNIFFSNAFEID
                     VKKYLGKSEDNEVEIKVGNERITIGIRNISNGKLERQKFLFPLDYENKLQHQLDKYFT
                     IESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISEN
                     IAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFET
                     KPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVF
                     MSLPVPVATMVREDATNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNE
                     SILKKCCFENGSLGDVKITNSNIVNNVFKNVSFRTVKINNVNTHSLKFINCHFFNVDM
                     IRVNFSKCLFHDCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAG
                     NNVKVAADAFLEILYSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCF
                     AAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
     gene            13092..13607
                     /locus_tag="QIE41_000011"
     CDS             13092..13607
                     /locus_tag="QIE41_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDS3528951.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLEQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            13825..14553
                     /gene="artP"
                     /locus_tag="QIE41_000012"
     CDS             13825..14553
                     /gene="artP"
                     /locus_tag="QIE41_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDS3528952.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            14571..15302
                     /gene="artJ"
                     /locus_tag="QIE41_000013"
     CDS             14571..15302
                     /gene="artJ"
                     /locus_tag="QIE41_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS3528953.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15309..16025
                     /gene="artQ"
                     /locus_tag="QIE41_000014"
     CDS             15309..16025
                     /gene="artQ"
                     /locus_tag="QIE41_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDS3528954.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPG"
     gene            16025..16693
                     /gene="artM"
                     /locus_tag="QIE41_000015"
     CDS             16025..16693
                     /gene="artM"
                     /locus_tag="QIE41_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDS3528955.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLAVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            16919..17650
                     /gene="artJ"
                     /locus_tag="QIE41_000016"
     CDS             16919..17650
                     /gene="artJ"
                     /locus_tag="QIE41_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDS3528956.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQEQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(17679..18806)
                     /gene="rlmC"
                     /locus_tag="QIE41_000017"
     CDS             complement(17679..18806)
                     /gene="rlmC"
                     /locus_tag="QIE41_000017"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDS3528957.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLAKQ"
     gene            complement(18847..19335)
                     /locus_tag="QIE41_000018"
     CDS             complement(18847..19335)
                     /locus_tag="QIE41_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDS3528958.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILLLLFVPSTSRRFFQ
                     LQ"
     gene            complement(19395..20240)
                     /gene="potI"
                     /locus_tag="QIE41_000019"
     CDS             complement(19395..20240)
                     /gene="potI"
                     /locus_tag="QIE41_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDS3528959.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMFTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(20237..21181)
                     /gene="potH"
                     /locus_tag="QIE41_000020"
     CDS             complement(20237..21181)
                     /gene="potH"
                     /locus_tag="QIE41_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDS3528960.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(21191..22324)
                     /gene="potG"
                     /locus_tag="QIE41_000021"
     CDS             complement(21191..22324)
                     /gene="potG"
                     /locus_tag="QIE41_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDS3528961.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFECVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(22419..23531)
                     /gene="potF"
                     /locus_tag="QIE41_000022"
     CDS             complement(22419..23531)
                     /gene="potF"
                     /locus_tag="QIE41_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDS3528962.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(23882..24358)
                     /locus_tag="QIE41_000023"
     CDS             complement(23882..24358)
                     /locus_tag="QIE41_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDS3528963.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(24446..25348)
                     /gene="rimK"
                     /locus_tag="QIE41_000024"
     CDS             complement(24446..25348)
                     /gene="rimK"
                     /locus_tag="QIE41_000024"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDS3528964.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(25409..26131)
                     /gene="nfsA"
                     /locus_tag="QIE41_000025"
     CDS             complement(25409..26131)
                     /gene="nfsA"
                     /locus_tag="QIE41_000025"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDS3528965.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(26115..26402)
                     /locus_tag="QIE41_000026"
     CDS             complement(26115..26402)
                     /locus_tag="QIE41_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDS3528966.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPTAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            26562..26819
                     /gene="grxA"
                     /locus_tag="QIE41_000027"
     CDS             26562..26819
                     /gene="grxA"
                     /locus_tag="QIE41_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDS3528967.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(26849..27226)
                     /locus_tag="QIE41_000028"
     CDS             complement(26849..27226)
                     /locus_tag="QIE41_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDS3528968.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            27496..29181
                     /locus_tag="QIE41_000029"
     CDS             27496..29181
                     /locus_tag="QIE41_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDS3528969.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(29356..29892)
                     /gene="rcdA"
                     /locus_tag="QIE41_000030"
     CDS             complement(29356..29892)
                     /gene="rcdA"
                     /locus_tag="QIE41_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDS3528970.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            29976..31184
                     /locus_tag="QIE41_000031"
     CDS             29976..31184
                     /locus_tag="QIE41_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3528971.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            31184..31999
                     /gene="ybjI"
                     /locus_tag="QIE41_000032"
     CDS             31184..31999
                     /gene="ybjI"
                     /locus_tag="QIE41_000032"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDS3528972.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            32084..32368
                     /locus_tag="QIE41_000033"
     CDS             32084..32368
                     /locus_tag="QIE41_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3528973.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(32409..33641)
                     /gene="mdfA"
                     /locus_tag="QIE41_000034"
     CDS             complement(32409..33641)
                     /gene="mdfA"
                     /locus_tag="QIE41_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDS3528974.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLFVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            33926..34522
                     /gene="ybjG"
                     /locus_tag="QIE41_000035"
     CDS             33926..34522
                     /gene="ybjG"
                     /locus_tag="QIE41_000035"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDS3528975.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            34580..35338
                     /gene="deoR"
                     /locus_tag="QIE41_000036"
     CDS             34580..35338
                     /gene="deoR"
                     /locus_tag="QIE41_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDS3528976.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(35385..36587)
                     /gene="dacC"
                     /locus_tag="QIE41_000037"
     CDS             complement(35385..36587)
                     /gene="dacC"
                     /locus_tag="QIE41_000037"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDS3528977.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            36834..37460
                     /gene="gstB"
                     /locus_tag="QIE41_000038"
     CDS             36834..37460
                     /gene="gstB"
                     /locus_tag="QIE41_000038"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDS3528978.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(37457..38572)
                     /gene="yliI"
                     /locus_tag="QIE41_000039"
     CDS             complement(37457..38572)
                     /gene="yliI"
                     /locus_tag="QIE41_000039"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDS3528979.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPEVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLNKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(38683..39066)
                     /gene="bssR"
                     /locus_tag="QIE41_000040"
     CDS             complement(38683..39066)
                     /gene="bssR"
                     /locus_tag="QIE41_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDS3528980.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            39279..40604
                     /gene="rimO"
                     /locus_tag="QIE41_000041"
     CDS             39279..40604
                     /gene="rimO"
                     /locus_tag="QIE41_000041"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDS3528981.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            40830..41216
                     /locus_tag="QIE41_000042"
     CDS             40830..41216
                     /locus_tag="QIE41_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDS3528982.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            41206..41535
                     /locus_tag="QIE41_000043"
     CDS             41206..41535
                     /locus_tag="QIE41_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDS3528983.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(41605..42933)
                     /locus_tag="QIE41_000044"
     CDS             complement(41605..42933)
                     /locus_tag="QIE41_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDS3528984.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQIVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGVSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSLYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(42941..45289)
                     /locus_tag="QIE41_000045"
     CDS             complement(42941..45289)
                     /locus_tag="QIE41_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDS3528985.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPAKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMINDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(45467..46378)
                     /gene="gsiD"
                     /locus_tag="QIE41_000046"
     CDS             complement(45467..46378)
                     /gene="gsiD"
                     /locus_tag="QIE41_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDS3528986.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAIFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(46381..47301)
                     /gene="gsiC"
                     /locus_tag="QIE41_000047"
     CDS             complement(46381..47301)
                     /gene="gsiC"
                     /locus_tag="QIE41_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDS3528987.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(47319..48857)
                     /gene="gsiB"
                     /locus_tag="QIE41_000048"
     CDS             complement(47319..48857)
                     /gene="gsiB"
                     /locus_tag="QIE41_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDS3528988.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKTQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(48877..50748)
                     /gene="gsiA"
                     /locus_tag="QIE41_000049"
     CDS             complement(48877..50748)
                     /gene="gsiA"
                     /locus_tag="QIE41_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDS3528989.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(50735..51700)
                     /gene="iaaA"
                     /locus_tag="QIE41_000050"
     CDS             complement(50735..51700)
                     /gene="iaaA"
                     /locus_tag="QIE41_000050"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDS3528990.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            51904..53139
                     /gene="moeA"
                     /locus_tag="QIE41_000051"
     CDS             51904..53139
                     /gene="moeA"
                     /locus_tag="QIE41_000051"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDS3528991.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDAGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VEAGEWVEVEPFNALFGGL"
     gene            53139..53888
                     /gene="moeB"
                     /locus_tag="QIE41_000052"
     CDS             53139..53888
                     /gene="moeB"
                     /locus_tag="QIE41_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDS3528992.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVDSARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(53975..54637)
                     /gene="fsa"
                     /locus_tag="QIE41_000053"
     CDS             complement(53975..54637)
                     /gene="fsa"
                     /locus_tag="QIE41_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDS3528993.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            54768..55667
                     /locus_tag="QIE41_000054"
     CDS             54768..55667
                     /locus_tag="QIE41_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDS3528994.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYYLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            55673..58105
                     /locus_tag="QIE41_000055"
     CDS             55673..58105
                     /locus_tag="QIE41_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDS3528995.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            58251..59066
                     /gene="ybiV"
                     /locus_tag="QIE41_000056"
     CDS             58251..59066
                     /gene="ybiV"
                     /locus_tag="QIE41_000056"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDS3528996.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            59218..60483
                     /locus_tag="QIE41_000057"
     CDS             59218..60483
                     /locus_tag="QIE41_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDS3528997.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQRIQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(60450..61610)
                     /locus_tag="QIE41_000058"
     CDS             complement(60450..61610)
                     /locus_tag="QIE41_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDS3528998.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            61848..63149
                     /locus_tag="QIE41_000059"
     CDS             61848..63149
                     /locus_tag="QIE41_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3528999.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSNLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHNPDKHPNLSKAELDFIRQ
                     DNEAPPIKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            63164..65527
                     /locus_tag="QIE41_000060"
     CDS             63164..65527
                     /locus_tag="QIE41_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDS3529000.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTLIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPVEEKRTLLVNGVEGSEWMR"
     gene            complement(65707..67302)
                     /locus_tag="QIE41_000061"
     CDS             complement(65707..67302)
                     /locus_tag="QIE41_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDS3529001.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIDG"
     gene            67521..68441
                     /gene="ldtB"
                     /locus_tag="QIE41_000062"
     CDS             67521..68441
                     /gene="ldtB"
                     /locus_tag="QIE41_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDS3529002.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(68499..69617)
                     /locus_tag="QIE41_000063"
     CDS             complement(68499..69617)
                     /locus_tag="QIE41_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDS3529003.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLFVMLPAW"
     gene            complement(69614..70081)
                     /gene="mntR"
                     /locus_tag="QIE41_000064"
     CDS             complement(69614..70081)
                     /gene="mntR"
                     /locus_tag="QIE41_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDS3529004.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            70267..70395
                     /gene="mntS"
                     /locus_tag="QIE41_000065"
     CDS             70267..70395
                     /gene="mntS"
                     /locus_tag="QIE41_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDS3529005.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            70667..72250
                     /gene="opgE"
                     /locus_tag="QIE41_000066"
     CDS             70667..72250
                     /gene="opgE"
                     /locus_tag="QIE41_000066"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDS3529006.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAMLLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGLIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPMQN
                     LAWGGVFKLKDTGYPVFRFAKDVIGNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(72299..72814)
                     /gene="ompX"
                     /locus_tag="QIE41_000067"
     CDS             complement(72299..72814)
                     /gene="ompX"
                     /locus_tag="QIE41_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDS3529007.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(72867..72932)
                     /locus_tag="QIE41_000068"
     CDS             complement(72867..72932)
                     /locus_tag="QIE41_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529008.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            73167..74054
                     /gene="rhtA"
                     /locus_tag="QIE41_000069"
     CDS             73167..74054
                     /gene="rhtA"
                     /locus_tag="QIE41_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDS3529009.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLIGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            74353..74856
                     /gene="dps"
                     /locus_tag="QIE41_000070"
                     /gene_synonym="pexB"
     CDS             74353..74856
                     /gene="dps"
                     /locus_tag="QIE41_000070"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDS3529010.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            75260..76006
                     /gene="glnH"
                     /locus_tag="QIE41_000071"
     CDS             75260..76006
                     /gene="glnH"
                     /locus_tag="QIE41_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDS3529011.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            76145..76804
                     /gene="glnP"
                     /locus_tag="QIE41_000072"
     CDS             76145..76804
                     /gene="glnP"
                     /locus_tag="QIE41_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDS3529012.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            76801..77523
                     /gene="glnQ"
                     /locus_tag="QIE41_000073"
     CDS             76801..77523
                     /gene="glnQ"
                     /locus_tag="QIE41_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDS3529013.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            77640..79865
                     /gene="ybiO"
                     /locus_tag="QIE41_000074"
     CDS             77640..79865
                     /gene="ybiO"
                     /locus_tag="QIE41_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDS3529014.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLSAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEDIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAAPLPPGEPT
                     L"
     gene            complement(79862..80788)
                     /gene="rlmF"
                     /locus_tag="QIE41_000075"
     CDS             complement(79862..80788)
                     /gene="rlmF"
                     /locus_tag="QIE41_000075"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDS3529015.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSVQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            81064..81324
                     /locus_tag="QIE41_000076"
     CDS             81064..81324
                     /locus_tag="QIE41_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDS3529016.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            81589..83871
                     /locus_tag="QIE41_000077"
     CDS             81589..83871
                     /locus_tag="QIE41_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDS3529017.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVANTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAARLNVMGE
                     KTHDAGRDKVKNERYGIAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            83913..84590
                     /gene="ybiX"
                     /locus_tag="QIE41_000078"
     CDS             83913..84590
                     /gene="ybiX"
                     /locus_tag="QIE41_000078"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDS3529018.1"
                     /translation="MMYHIPGVLSPKDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            84664..84930
                     /gene="ybiI"
                     /locus_tag="QIE41_000079"
     CDS             84664..84930
                     /gene="ybiI"
                     /locus_tag="QIE41_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDS3529019.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            85195..85455
                     /locus_tag="QIE41_000080"
     CDS             85195..85455
                     /locus_tag="QIE41_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDS3529020.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(85595..86680)
                     /gene="hcxB"
                     /locus_tag="QIE41_000081"
     CDS             complement(85595..86680)
                     /gene="hcxB"
                     /locus_tag="QIE41_000081"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDS3529021.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(86821..87783)
                     /gene="ybiB"
                     /locus_tag="QIE41_000082"
     CDS             complement(86821..87783)
                     /gene="ybiB"
                     /locus_tag="QIE41_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDS3529022.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(87811..89961)
                     /gene="dinG"
                     /locus_tag="QIE41_000083"
     CDS             complement(87811..89961)
                     /gene="dinG"
                     /locus_tag="QIE41_000083"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDS3529023.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELLDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVGNGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            complement(90132..90413)
                     /locus_tag="QIE41_000084"
     CDS             complement(90132..90413)
                     /locus_tag="QIE41_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDS3529024.1"
                     /translation="MRLEWKPMSLSDREQIMEYISKDNPLAAIALDNQFEAIAEQACE
                     RPEMYRAGRVAGTHEAVVRPHYVLVYQVEGDTLSVLRVLHTALQWPPAE"
     gene            complement(90413..90853)
                     /locus_tag="QIE41_000085"
     CDS             complement(90413..90853)
                     /locus_tag="QIE41_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529025.1"
                     /translation="MAPETIDHSTLHKLVESGVVDAAHVIGTQGGWSLTVKYGHTERP
                     LAAQRSRQIRLFKRLETVVNYLKDVGIARFEVDASNYDPDGQKKTTRPDRAEALKRAH
                     EAAAYDAWFREQVQAAIDDPRPALSHEEAKSLFAARKKALLKGD"
     gene            complement(91010..92374)
                     /gene="rhlE"
                     /locus_tag="QIE41_000086"
     CDS             complement(91010..92374)
                     /gene="rhlE"
                     /locus_tag="QIE41_000086"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDS3529026.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            92603..93274
                     /gene="cecR"
                     /locus_tag="QIE41_000087"
     CDS             92603..93274
                     /gene="cecR"
                     /locus_tag="QIE41_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDS3529027.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            93277..94272
                     /gene="hlyD"
                     /locus_tag="QIE41_000088"
     CDS             93277..94272
                     /gene="hlyD"
                     /locus_tag="QIE41_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDS3529028.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            94265..96001
                     /locus_tag="QIE41_000089"
     CDS             94265..96001
                     /locus_tag="QIE41_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3529029.1"
                     /translation="MNDAVITLNGLEKRFPGMNKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFSRLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            95994..97127
                     /locus_tag="QIE41_000090"
     CDS             95994..97127
                     /locus_tag="QIE41_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3529030.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            97138..98244
                     /locus_tag="QIE41_000091"
     CDS             97138..98244
                     /locus_tag="QIE41_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3529031.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNSNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(98206..98616)
                     /locus_tag="QIE41_000092"
     CDS             complement(98206..98616)
                     /locus_tag="QIE41_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDS3529032.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            98749..99510
                     /locus_tag="QIE41_000093"
     CDS             98749..99510
                     /locus_tag="QIE41_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDS3529033.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            99507..100748
                     /gene="clsB"
                     /locus_tag="QIE41_000094"
     CDS             99507..100748
                     /gene="clsB"
                     /locus_tag="QIE41_000094"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDS3529034.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDAHIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            100748..101704
                     /locus_tag="QIE41_000095"
     CDS             100748..101704
                     /locus_tag="QIE41_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDS3529035.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(101740..102129)
                     /locus_tag="QIE41_000096"
     CDS             complement(101740..102129)
                     /locus_tag="QIE41_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529036.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGMVATAELALVIIMD
                     AALMSERPVWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT        24956 a        26944 c        25538 g        24777 t
ORIGIN      
        1 ggaacgcctc ggcgattttg acgccaccaa caccggtgcg gtttgtaatt aaagcgcaag
       61 aatatcttta aatgaaaatg aataccctac cgatccttat gcataacatt tccttttaat
      121 tgcaacttta ctcgtccttc cgctttcagt agtgtgtcat cgcgcatttc gacttcatct
      181 ttgcaaggac tgggttttca tgttttctgg gctgttaatc attctggttc ccctgattgt
      241 gggttacctc attcctcttc gccaaaaagc tgcattaaga gttattaatc agctattaag
      301 ctggatggtt tacctcattc tcttttttat gggtatcagt ctggcgtttc tcgataacct
      361 cgccagtaac ctgttggcga ttctgcatta ttccgccgtc agcattacca ttattttact
      421 ctgcaatatt attgcgttgt tgtggctgga gcgcggtctg ccgtggcgca accaccacca
      481 gcaagaaaaa ctcccgtcgc gtattgcgat ggcgctggag tcgctaaaac tgtgcggcgt
      541 agtagtgatt ggttttgcca ttggcctgag tggactggct ttcttacaac atgcgaccga
      601 agccagtgaa tacaccttaa ttctgctact tttcctcgtc ggtattcagt tgcgcaataa
      661 tggcatgacc ttaaagcaga ttgtccttaa tcgccgggga atgattgtcg ccgtggtggt
      721 ggttgccagt tcattaattg gtggtttaat taacgccttt attcttgatc ttcccatcaa
      781 taccgcgctg gcaatggcct ctggtttcgg ctggtattct ctttccggta ttttattgac
      841 tgaatctttt ggtccggtaa tcgggagcgc ggcgtttttt aatgatctgg cccgtgagct
      901 gattgcaatt atgttgatcc ctgggctgat tcgccgcagc cgctctaccg cactgggctt
      961 atgcggtgcg acgtcgatgg atttcaccct gcccgttctt caacgtactg gcgggctgga
     1021 tatggtcccg gcggcaattg ttcacggttt tattcttagc ctgttagtgc cgatcctcat
     1081 cgcctttttc tctgcgtaat acctctctgg cggtagatcc ctgccgccaa aattgcgcta
     1141 aatcaatctc ccttaaagtt gcatgaaaaa tcccttttat ccctgcgtta agcgtcttaa
     1201 ccttaaacat gtatattaaa tataacttta aaaggtgtga tcatgttttg tgtgcaatgt
     1261 gaacaaacta tccgtactcc ggcaggaaac ggctgctcat acgcgcaggg gatgtgtggt
     1321 aaaacggcgg aaacctctga ccttcaggat ttactcatcg cggcgctgca agggctttcg
     1381 gcctgggcgg taaaagcgcg tgaatacggc atcatcaacc acgatgtaga cagctttgcg
     1441 ccacgcgcat ttttctcaac cctgaccaac gttaacttcg attctccgcg tatagtcggc
     1501 tacgctcgtg aagcgattgc cctgcgcgag gcgctgaaag cacaatgcct ggctgtagat
     1561 gccaacgccc gcgtcgataa cccgatggct gacctgcaac tggtgagcga tgatctcggc
     1621 gagctgcaac gtcaggcagc agaatttacc cctaacaaag ataaagcggc gattggcgaa
     1681 aacattctcg gcctgcgtct gctgtgcctg tatggcctga aaggtgcggc ggcctatatg
     1741 gaacacgcgc atgttctcgg tcaatacgac aacgatattt acgcccagta ccataaaatc
     1801 atggcgtggc tgggaacctg gcctgccgat atgaacgcgc ttcttgagtg ttcaatggaa
     1861 atcggccaga tgaacttcaa agtgatgagc attctggacg caggcgaaac cggtaaatac
     1921 ggtcacccga ccccaaccca ggtcaacgtc aaagcgacgg caggtaaatg cattctgatt
     1981 tccggtcacg atctcaaaga tctctacaac ctgctggaac agaccgaagg cacgggcgtt
     2041 aatgtctaca cccacggcga aatgctgcct gcgcatggct acccggagct gcgtaaattc
     2101 aagcatctgg tcggtaacta cggcagcggc tggcagaatc agcaagtgga gttcgctcgt
     2161 ttccctggcc ccatcgtgat gacctcgaac tgcatcatcg acccaaccgt aggcgcttat
     2221 gacgatcgta tctggacccg cagcatcgtt ggctggccgg gtgtgcgtca tctggatggc
     2281 gaagatttct ctgcggtcat cgcccaggcg caacaaatgg cgggcttccc gtacagcgaa
     2341 attccgcacc tgatcaccgt gggctttggt cgccagacac tgcttggcgc agcggatacg
     2401 ctgattgatc tggtaagtcg tgaaaaactg cgtcatatct tcctgcttgg cggctgtgac
     2461 ggcgcacgcg gcgagcgtca ctacttcacc gatttcgcca ccagcgtgcc ggatgactgt
     2521 ctgatcctga ctctcgcctg tggtaaatat cgctttaaca aactcgagtt tggcgatatc
     2581 gaaggtctgc cgcgtctggt agatgctggt cagtgtaacg atgcttactc agcgattatt
     2641 ctggcggtaa cgctggcaga gaaactgggc tgcggagtca acgatctgcc gctgtcgctg
     2701 gtgctctcgt ggtttgaaca gaaagcgatt gtcattctgc tgacactgct ttctctgggt
     2761 gtgaaaaata tcgtcaccgg cccgactgca cctggtttcc tgacaccaga cctgctggcg
     2821 gtgctgaacg agaaattcgg cctgcgttct atcaccactg ttgaagaaga catgaagcaa
     2881 ctgttgagcg cgtaaggagt ttgtcgatga cgatgccaac gaatcaatgc ccgtggcgga
     2941 tgcaggttca tcacattaag caagaaacgc cggatgtgtg gacgatttcc ctgatttgcc
     3001 acgattacta cccatatcgc gccgggcaat atgcactggt cagcgtgcgt aactcagccg
     3061 aaacgctgcg tgcttacacc atttcctcca cgccaggcgt gagtgaatac atcaccctga
     3121 ctgtgcggcg gattgatgac ggtgtcggct cccagtggct gacgcgcgat gtaaaacgcg
     3181 gtgattatct ctggctttcg gacgcgatgg gggaatttac ctgcgacgat aaagcagaag
     3241 ataaattcct gttgctggcg gcaggctgcg gcgtcacgcc gattatgtcg atgcgtcgct
     3301 ggctggcgaa gaaccgtccg caggccgatg tgcaggtgat ctacaacgtg cgttcgccgc
     3361 aggatgttat tttcgccgat gagtggcgta actatccggt gacgctggtg gcagaaaata
     3421 acgttaccga aggctttatc gctggtcgtc tcactcgcga actgctgact cgcgttcctg
     3481 acttagcttc acgtaccgtg atgacctgcg gcccggctcc gtatatggat tgggtagagc
     3541 aggaagtgaa agcgctcggc gtgacgcgtt tctttaaaga gaaattcttc accccagtag
     3601 cggaagcggc gaccagcggt ctgaaattca ccaaactgca accggcacga gaattttacg
     3661 ccccggttgg caccacgcta ctggaggcgc tggaaagcaa taacgttccg gttgtcgctg
     3721 cctgccgtgc gggtgtttgc ggctgctgta agacgaaagt ggtttccggt gaatatacgg
     3781 tgagcagcac aatgacgctg accgacgccg aaatcgctga aggttacgtg ctggcctgct
     3841 cctgccatcc gcagggagat ttggttctcg cataatcgcc ttatgcccga tgatattcct
     3901 ttcatcgggc tatttaaccg ttagtgcctc ctttttctcc catcccttcc ccctccgtca
     3961 gatgaactaa acttgtaacc gttatcacat tcaggagatg gagaaccatg aaacaaacgg
     4021 ttgcagctta tatcgccaaa acactcgaat cggcaggcgt gaaacgcatc tggggagtca
     4081 cgggcgactc tctgaacggt cttagcgaca gtcttaatcg catgggcacc atcgagtgga
     4141 tgtccacccg ccatgaagaa gtggcggcct ttgccgctgg tgctgaagca caactttcag
     4201 gagaactggc ggtctgtgcc ggatcgtgcg gccccggcaa cctgcactta atcaacggcc
     4261 tgttcgattg ccaccgcaat cacgttccgg tactggcgat tgccgctcat attccctcca
     4321 gcgaaattgg cagcggctat ttccaggaaa cccacccaca agagttattc cgcgaatgta
     4381 gtcactattg cgagctggtt tccagcccgg agcaaatccc acaagtgctg gcgattgcca
     4441 tgcgcaaagc ggtgctaaac cgtggcgttt ccgttgtcgt gttaccgggc gacgtggcgt
     4501 taaaacctgc gccagaaggg gcaacctcgc actggtatca tgcgccacag ccagtcgtga
     4561 cgccggaaga agaagagtta cgcaaactgg cgcaactgct gcgttattcc agcaatatcg
     4621 ctctgatgtg tggcagcggc tgcgcggggg cgcataaaga gttagttgag tttgccggga
     4681 aaattaaagc gcctattgtt catgccctgc gcggtaaaga acatgtcgaa tacgataatc
     4741 cgtatgatgt cggaatgacg ggattaattg gcttctcgtc aggtttccat accatgatga
     4801 acgccgacac gttagtgcta ctcggtactc agttccccta ccgcgccttc tacccgaccg
     4861 atgccaaaat cattcagatt gatatcaacc cagccagcat cggcgctcac agcaaggtgg
     4921 atatggcact ggtcggcgat atcaagtcaa ctctgcgtgc attgctgcca ttggtggaag
     4981 aaaaagccga tcgcaagttt ctggataaag cgctggaaga ttaccgcgac gcccgcaaag
     5041 ggctggacga tttagctaaa ccgagcgaga aagccattca cccgcaatat ctggcgcagc
     5101 aaattagtca ttttgccgcc gatgacgcca tctttacctg tgacgttggt acgccaacgg
     5161 tgtgggcggc acgttatctg aaaatgaacg gcaagcgtcg tctcttaggt tcgtttaacc
     5221 acggttcgat ggctaacgcc atgccgcagg cgctgggtgc tcaggcaacc gagccggaac
     5281 gtcaggtggt cgccatgtgc ggcgatggcg gttttagcat gttgatgggc gatttcctct
     5341 cagtagtgca gatgaaactg ccggtgaaaa ttatcgtctt taataacagc gtgctgggct
     5401 ttgtggcgat ggagatgaaa gctggtggct atttgactga cggcaccgaa ctacatgaca
     5461 ccaactttgc ccgcattgcc gaagcgtgcg gaattacggg tattcgcgtg gaaaaagcct
     5521 ctgaagtcga tgaagccctg caacgcgcct tttccatcga cggtccggtg ctggtggatg
     5581 tggtggtcgc caaagaagaa ttagccattc caccgcagat caaactcgaa caggccaaag
     5641 gttttagcct gtatatgttg cgcgcaatca tcagcggacg cggtgatgaa gtgatcgaac
     5701 tggcgaaaac aaactggcta aggtaaaaag ggtggcattt cccgtcataa taaggacatg
     5761 ccatgattga tttacgcagt gataccgtta cccgaccgag ccgcgccatg ctcgaagcga
     5821 tgatggccgc cccggttggg gacgacgttt acggagacga ccctaccgtt aatgctctgc
     5881 aggactatgc agcagagctt tccggtaaag aagccgccat ttttctgcct accggcactc
     5941 aggccaacct ggtcgctctg ctcagtcact gtgaacgcgg cgaagagtat attgtcggtc
     6001 aggccgcgca taactatctg tttgaagccg gtggcgcggc ggtgctgggc agtattcaac
     6061 cgcaacccat cgacgcggct gccgacggca cgctaccgct ggataaagtg gcgatgaaaa
     6121 tcaaacccga cgatatccat ttcgcccgca ccaaattact cagtctggaa aacacccaca
     6181 acggcaaagt gctgccgcgg gaatacctga aagaagcatg ggaatttact cgcgagcgca
     6241 atctggcgct gcatgttgac ggtgcgcgca tctttaatgc cgtggtggct tacggctgcg
     6301 aactgaaaga gatcacgcaa tattgtgatt cgttcaccat ttgcctgtcg aaaggacttg
     6361 ggacgccagt cggctcgtta ctggtcggta atcgtgatta cattaaacgt gccatccgct
     6421 ggcggaaaat gaccggtggc gggatgcgcc agtccggcat tctggctgcc gccgggatgt
     6481 atgccctgaa aaataacgtc gcgcgcttac aggaagacca cgacaacgcc gcctggatgg
     6541 cggagcagtt gcgtgaagca ggcgcggatg tgatgcgtca ggacaccaat atgctgtttg
     6601 ttcgcgtcgg ggaagaaaat gctgccgcgt taggcgaata catgaaagcg agaaacgtgc
     6661 tgattaacgc ctcgccgatt gtccgcctgg tgacgcatct cgacgtctcg cgcgagcaac
     6721 tggctgaagt cgccgcccac tggcgcgcat tcctggcgcg ttaaggaggg agacgtgccg
     6781 caacgcattt tagttctcgg tgccagtggc tacattggtc agcatctggt gcgaacactc
     6841 agccagcagg ggcatcagat cctggcggcg gcacgtcatg tcgacaggct tgcaaagctg
     6901 caactggcaa atgtcagttg ccataaagtc gatctcagct ggccggataa ccttccggcc
     6961 ctgttgcagg atatcgatac ggtctatttt ctggtgcaca gcatgggcga aggcggcgat
     7021 tttatcgctc aggagcgcca ggtggctctc aacgtccgcg atgcgctacg tgaagtccca
     7081 gttaagcaat taatctttct cagttcgttg caggccccgc cacatgagca atcggaccat
     7141 ctgcgtgccc gtcaggctac ggcggatatt cttcgtgaag cgggtgtccc ggtgaccgaa
     7201 ttgcgtgccg gaataatcgt tggtgcaggt tcagcggcgt tcgaagtcat gcgcgatatg
     7261 gtctacaacc tgccagtctt aacgccgcca cgctgggtac gttcacgcac cacgcccatc
     7321 gcgctggaaa acttgctgca ctatctggtg gcgctgttag atcatccggc ctgcgaacat
     7381 cgcatcttcg aagccgccgg accagaagtg ctcagttatc agcaacagtt tgaacatttt
     7441 atggcggtga gcggtaagcg tcgctggttg atccccatcc ctctccccac ccgctggatt
     7501 tcggtgtggt ttctcaatgt gattacttcc gtaccaccca ccaccgccag ggcgttgatt
     7561 caggggctga aacacgatct gctggcggat gacaccgagc tacgtgcgct catcccacaa
     7621 cggctgatcg ctttcgatga cgcggtacgt cgcaccctga aagaggaaga aaagctggtc
     7681 aactccagcg actggggcta cgacgctcag gcctttgccc gctggcgacc ggagtacggt
     7741 tatttcgcca aacaggcggg gtttaccgtt aaaacgtccg ccagccttgc ggctttatgg
     7801 caggtggtga atcaaatcgg cggtaaagag cgttatttct ttggcaatat tttgtggcaa
     7861 acccgggcgt tgatggaccg cgcgatcggt cataagctgg cgaaaggccg cccggagcgt
     7921 gaatatttgc agaccggcga tgcggtggat agctggaaag tgattgtcgt tgaaccggaa
     7981 aaacaactta cgctgttatt tggcatgaaa gcgccggggc tgggacgact gtgttttacc
     8041 ctggaagata aaggcgacta tcgtactatc gatgttcgcg cattctggca tccgcacggt
     8101 atgccggggc tgttttactg gttattgatg atccccgcgc atctgtttat ttttcgcgga
     8161 atggcaaaac gaatcgccag actggcagaa caaagcacag attaatcgac aaaacctgcg
     8221 ttattctttc atgtttccca ttgcatcatg ggggaaatca cggaagaatg cgcacgtcat
     8281 tttctcaaca acagtggatt gatatgaagg tactggttac cggcgccacc agcggcttag
     8341 gtcgaaacgc ggtagagttt ttatgccaga aaggcatcag cgtgcgagcg accggtcgca
     8401 acgaggcaat gggtaaattg ctggaaaaaa tgggcgcaga gtttgttccg gcggatctga
     8461 ccgagctggt ctcatcacaa gctaaagtga tgctcgcggg cattgatacg ctgtggcact
     8521 gctccagctt tacctcgccc tgggggacac aacaggcttt cgatctggct aacgttcgcg
     8581 ccactcgccg cctgggtgaa tgggctgtcg cctggggtgt gcgtaacttt attcatatct
     8641 cttccccctc cctgtacttc gattatcacc accatcgcga tattaaagaa gattttcgcc
     8701 ctcaccgctt tgccaacgag tttgcccgca gcaaagcagc cagcgaagaa gtgatcaata
     8761 tgctttcgca ggcgaatcca caaacgcgct ttactattct gcgcccacaa agtctgttcg
     8821 gaccgcacga taaagtcttt atcccccgtc tggcgcatat gatgcaccac tacggcagca
     8881 ttctgttacc gcacggcggc agtgcgctgg tcgatatgac ctactatgaa aatgccgtgc
     8941 acgccatgtg gctggcaagc caggaagcct gcgataagct accttccggg cgtgtgtaca
     9001 acatcaccaa cggcgagcat cgcacactgc gcagcatcgt gcagaagctg atcgacgagt
     9061 tgaatattga ctgtcgtatt cgttccgtcc cctacccgat gctggatatg atcgcccgca
     9121 gcatggagcg tttaggccgc aagtcagcaa aagagccgtc gctgacccac tacggcgtct
     9181 ccaagttgaa ttttgacttt acgctggata ttacgcgtgc gcaggaagag ttaggttatc
     9241 aaccagtcct caccctggat gaaggtatcg agaaaactgc cgcctggcta cgcgaccacg
     9301 gaaaactgcc gcgctaatcc tgcccgtatt tctccagcaa cgcttcggca atcgcctgag
     9361 tttctgcatc cgcttcgccg ttgtataacg tcgggcggaa atgcatctgg aacgccataa
     9421 tcacgcgccg ctgctcgcgc ggtgtcatat caggtttaac gtcataaccg tagcgcgcca
     9481 aaagctccag caatgatgcg gtatctaccg gtgtgtgcgg cgcgcgcccg gcaaggtaaa
     9541 agttcacccg ttgcgcatcc ggccaggcac caatcccctg ctgcgccagt tgttgccagg
     9601 ggaataacgg ccccggatcg tctttgcgct gcggcgcaat atccgcatgc gccactacgt
     9661 tttccggctt gatgtgataa cgggcaataa tatctttcgc cagcggaata agcgcctgaa
     9721 tctgtgcctg ttcaaacggg gcaaaatatt tcactccgga tgatttctgc cagccacggt
     9781 tttccagctc aatgccaata gaggtgtcgt taaggcgcgt tgccccgcgc caggcgctaa
     9841 tccccgcatg ccaggccagc tcctgttccg gcaccagttg ccagatgcgc ggtttaccgt
     9901 tgtatcgcgg tggcacagca ggcaccagat aatgcgaact gacctgctta tcggtcagtg
     9961 tcgccagcga gctatcaaaa tcatctgcgg tgtagtggat aaccagcact ttaatgcgcg
    10021 gatacgccgc ctgcgcctgg cgtcgggtat caagctgata tccctctttc tcgacaatgc
    10081 ccttttcgcc ggcacaccct gccagtaaca gagcggcggc gaccagccag aagactcttc
    10141 tcatcgacgc gttttcaccg ccgtaccgct gacgctaacc atcagcatac tgccattttg
    10201 cccgaccgtt tcgtagtcga tatcaatacc gactacggca tcagccccca gcgcccgggc
    10261 ctgatcgccg agctcctcaa aggcaatctc ccgcgcttta cgcaattctt tttcataagc
    10321 cccggagcgt ccgccaacga tatcgcggat accggcaaag aaatcacgga aaatattggc
    10381 acctaaaatc gcttcgccgg tcaccacacc gcaatattcg acgatggtct ggccttccag
    10441 agttggggtt gtagaaaatt gcatgatcga tctccttgtt tgttaagcct tcgatctcta
    10501 aagcattatc ggactgatat gccatgttgg gaagaaggtt ttgatcagtt taggaaatca
    10561 tctcattgtg tcaaaaacag gtacataagc ctgtacaatt atccgttgca taacccgata
    10621 ttagttttat tatggagcac cgcttacctg tcgtattatg tagtaccagc aacattaatg
    10681 agtgggaaga gtcaacttat attgtaaatg attcctaaag tcttccccat cttgcgcctg
    10741 aggtatatat ctctggattg ctttttggtc agccgccgca aaacaagaaa gtaataagat
    10801 ctcaatactt ttgtcattaa tataagcgaa gatcattctt gcaaattcgt atataaacgc
    10861 ccgatcctga gcgcttaata gtttaataca atatttattt ttaagagaat aaagtatttc
    10921 aaggaaagca tccgcagcaa ccttcacgtt attcccggcg ctcatattat atatatccct
    10981 tgcacagata gagtacagag atagtaaaca gccatacaaa taatcattta ttaactcttt
    11041 tgtccatttt actggcaacc atggttttat cttgacacca tgcatactac agtcatgaaa
    11101 aagacatttg ctgaaattaa ctcttatcat atcaacatta aaaaaatggc aattaataaa
    11161 ttttaaagaa tgagtattca cattgttaat tttaacagtt ctaaacgaaa cgtttttgaa
    11221 tacattgttt actatattgc tatttgttat tttaacatca cctaaactgc cgttttcaaa
    11281 acaacatttt ttcaatatgc tttcattcac aacagtgtgt gataaataga gattacttat
    11341 gttttgcttt attaatgcca cgtcattaaa atgactatat ttaataaagc aaccatcatt
    11401 gagtagtgtt ttaagattga ctaaattagt tgcatcttca cgtaccatcg tggcaactgg
    11461 aacaggcaac gacatgaaaa cttcttcatt caccatcaag tggatcatgc attgcgttcc
    11521 ctttgtcata tccacactta cgctacaaat atcgttgtaa tgcgaagagt gcatttcagg
    11581 aaacaaatta aacacggtat cttgaaaggc actaaatcgt gttaataaat cctgataatg
    11641 aacaggtttt gtttcaaaat tatcatcagc aaatccaagc actattgcat gctctacatc
    11701 taacgcagtt tcaaatgact cttcttctgt aaaatcatta tttaaaacct cacctgacgt
    11761 attatcactg tcataaaaag tgctcatcaa tgcgatattg tttgttacag cgtcttttgc
    11821 aatattctca ctaattgcat atcgcctgct ataatcataa aaatgaataa acgcatcctt
    11881 tataaaggat atatattgct ctttgttaaa gtcaatacga ctgtataatt tttcaaaaat
    11941 cacattcgca atcttactac ccatagtatg cctgtataat aatgggtgcg attcaatcgt
    12001 aaaatacttg tctaattgat gttgtaattt attctcatag tccagtggga aaagaaattt
    12061 ttgtctttca agtttcccat tcgatatatt acgtattcca atggtaattc tttcattacc
    12121 cacttttatt tcaacttcgt tatcttctga tttgccaaga tattttttaa catcaatctc
    12181 gaaagcatta ctaaaaaata tattaaattg ataattcgct gatggaatat tttccgctag
    12241 caactcatca ttttttatga gatcactatc agcatttatt gctaatatca gatgcaagta
    12301 gatggtgtcc catgtagaca actcaccctt acgactggaa tttaatatcg cgaaaaattc
    12361 cctcagtgaa tcgaggaaat tatcactttg attcagttgt gtgcctggaa atgatgatgc
    12421 ttgatcagat atattgttta tcatattaaa aatcacccaa gggaaaagat attccactga
    12481 actcctcgtg ctttattatt ataaaagcac aacgcattta tactcatatt aattaacgaa
    12541 acactgatac agcttgattt attcttcgct caaactcatt agcttaaatt ctttacccac
    12601 aactgtagca tatataatat tatcaaaagt caaagctggg actatcaaat ttcgctcatt
    12661 ttcaaaaata caatgtaatt ttagcaatca tcttaatcgc aggatctctt attacatgtg
    12721 aaaaattctg taatgattag acaataaccg cgttcattta tcacgcgtta taaatgtgaa
    12781 actggcttct tgcagatgct cttaacctct acaggaggat ttatcccgcc atttcagaac
    12841 tatcatattt accttaaatg atcatttcag ccatataaca cgctcaacct cacaaatggt
    12901 ttacactgtc tattgtttgg gtaatttaag taagtcagct acgagcaacc ttgagtgggt
    12961 agccagcaat ggactcaaac ctaatcaggt gttcagattg tttcagaatt gtactaaaaa
    13021 caatgtatta aataatctga tagtgtttgg tcgtagtgtg acacagacag caagctaata
    13081 aggaaatgat tatgcgctac cgttcactat ctctacttat tccatgtgca ctgctgctga
    13141 gtgcctgtac caccgtcacg ccagcttata aagataacgg cccccgcacc ggttcctgtg
    13201 tccagggtgg ccctgacagc gtggctcaac aattctatga ctaccgcatt caacatcgca
    13261 gcaatgacat taccgccctg cgcccctatt tgagtgacaa actggcaacc ctacttagcg
    13321 atgccagccg cgataatagt catcgtgaat tgttaagcag cgatccgttc tccagtcgaa
    13381 ccaccttgcc agacagcgcc cacgttgcca gtgcatcgac tatccctaat cgtgatgccc
    13441 gtaacattcc attgcgcgtg gatctcgaac agggtgacca gggatggcag gatgaagtgt
    13501 taatgattca ggaaggccag tgttgggtca tcgacgatgt gcgctactta ggcggtagcg
    13561 ttcacgccac agcgggtacg ctccgtcagt ccatcgagaa tcgctaaagc cttgatcaac
    13621 caataaaccc cgtaaattgt ccggcatttc gtgaggaatg ccgacattta tgctcgccga
    13681 ccaccgcccc cgttattttg tgctatgttt attgaataat gcgctttgct tttaactttt
    13741 aaagcagaaa tattgcataa ttattctgtc aaaggtacta tcttcgacct caatagctat
    13801 cagactgcca gtatacgagt gtcaatgagt attcaattaa acggcattaa ttgcttctac
    13861 ggcgcgcatc aggcgctgtt cgatatcacg ctggattgcc cacagggcga aacgctggtg
    13921 ttacttggcc ccagcggcgc gggtaaaagc tcgctgctgc gtgtactcaa tctgcttgag
    13981 atgccgcgct ccggtacgct caacattgca ggcaaccatt tcgatttcac caaaacaccc
    14041 tctgacaaag cgattcgcga tttgcgtcgt aacgttggca tggtgtttca gcaatacaac
    14101 ctgtggccgc atctgaccgt gcagcaaaac ctgattgaag cgccctgccg tgtactgggg
    14161 ttgagcaaag atcaggcgct ggcccgtgca gaaaaactgc tggaacgtct gcgtctcaaa
    14221 ccttatagcg atcgttaccc gctgcatctt tctggtggtc agcagcagcg tgttgctatt
    14281 gcccgtgcgt tgatgatgga accgcaggta ctgctgttcg atgaaccgac cgccgcactg
    14341 gacccggaaa ttacggcaca aatcgtcagc atcattcgtg agttggcaga aacgaatatt
    14401 acccaggtga tcgtcaccca cgaagttgaa gtggcgcgta aaaccgccag ccgagtggtg
    14461 tatatggaaa acggtcatat cgtagaacaa ggcgacgcga gctgctttac cgagccgcaa
    14521 accgaagcat ttaaaaacta tctctctcac taatattcgg gataacaaca atgaaaaaag
    14581 ttctgattgc cgcgttaatt gcaggtttta gtctttccgc cacagctgcc gaaactattc
    14641 gttttgctac cgaagcctcc tatcctccgt ttgaatcgat tgatgcaaac aaccagatcg
    14701 ttggttttga cgtcgacctg gcacaggcac tgtgtaaaga gattgatgca acctgtactt
    14761 tctctaacca ggcgtttgac agcctgatcc caagcctgaa attccgtcgc gtagaagcag
    14821 tgatggcggg catggatatc actccggagc gtgaaaagca ggtgctgttt accacccctt
    14881 actatgacaa ctctgccctg tttgtgggtc agcaaggcaa atacaccagt gttgatcagc
    14941 tgaaaggcaa aaaagtcggc gttcagaacg ggacgacaca ccagaagttc attatggata
    15001 agcacccgga aatcaccacc gttccgtatg acagctacca gaacgcaaaa ctggatctgc
    15061 aaaatggtcg tatcgacagc gtatttggtg acaccgcagt ggtaaccgaa tggctgaaag
    15121 acaacccgaa actggcggca gtgggcgata aagtgaccga taaagattat ttcggtactg
    15181 gcctcggcat cgcggtacgt cagggcaaca ctgaactgca gcagaaactc aacactgcgc
    15241 tggaaaaagt gaagaaagat ggcacttacg aaaccatcta caacaaatgg ttccagaagt
    15301 aattcctgat gaatgaattt tttcctttag caagcgccgc cgggatgacc gtcggccttg
    15361 ccgtttgtgc attgattgtc gggctggcgc tggcgatgtt ctttgcggta tgggagtctg
    15421 caaaatggcg tcctgtcgcg tgggcaggtt cagcgctggt aaccattctg cgtggcctgc
    15481 cagaaattct ggtggtgctg tttatctatt ttggctcctc gcagctgctg ctgacgcttt
    15541 cggatggttt cactatcaat cttgggttcg tgcagatccc ggtgcagatg gacattgaga
    15601 acttcgacgt tagcccgttc ctttgtggtg tcatcgctct gtcgctgctg tatgccgcct
    15661 atgcctcgca aacgctgcgc ggcgcgttga aagcggtgcc gatgggtcag tgggaatccg
    15721 gccaggcgct ggggctgtcg aaatcggcta tctttttccg tctggtgatg ccgcagatgt
    15781 ggcgtcatgc gctgcctggt ctcggtaacc agtggctggt gctgctgaaa gataccgcgc
    15841 tggtcagttt gattagtgtg aatgatttaa tgctacaaac caaaagcatc gctacccgta
    15901 cccaggaacc atttacctgg tacattgtgg cggcggcgat ttacctggtg atcaccctgc
    15961 tcagtcagta cattctcaaa cgcattgacc tgcgcgcgac acgttttgag cggaggcctg
    16021 gctaatgttt gagtatttac ccgaactgat gaaagggctg cacaccagcc tgacgctaac
    16081 cgttgcctcg ctgattgtgg cactgattct ggcattgatt tttaccatca tcctgacgct
    16141 gaaaacgccg gtactggtgt ggctggtgcg gggttatatt acgctgttta ccggtacgcc
    16201 gctgctggtg cagattttcc tgatttatta cgggccgggc cagttcccga cgttgcagga
    16261 gtatccggca ctgtggcatt tgctgtcaga accgtggctg tgtgcgctga ttgcgttgtc
    16321 gctgaacagt gcagcgtata ccacgcagct gttttacggt gccattcgtg cgatcccgga
    16381 aggtcagtgg cagtcctgta gcgccctggg aatgagcaaa aaagatacgc tggcgatcct
    16441 gctgccgtat gcctttaaac gctcgctctc ttcttattcc aacgaagtgg tgctggtgtt
    16501 caaaagtacc tctctggctt acaccattac gctgatggaa gtgatgggat acagccagtt
    16561 gttgtacgga cgcacctacg atgtcatggt gttcggtgcg gcagggatta tttacctggc
    16621 cgtcaacggc ctgctgacgc tgatgatgcg tctgatcgag cgcaaagcac tggcattcga
    16681 acggcggaat taatgtggct gcgtgctgat gccggatgcg gcgtgaacgc cttatccggc
    16741 ctacatagtg cataaattct tataatgaag acggacaacc cactaagttg tccgtctttt
    16801 tttattccat gtaaattatt taatcatatt tattgcatat aaattcactt gatggcattg
    16861 ttatcccatg ccgcagacac ggccaaaaat cataagattg acagacggga gttccatcat
    16921 gaaaaagtta gttcttgccg ctttacttgc ttcctttact ttcggtgctt ctgccgcaga
    16981 gaaaatcaat tttggcgttt cagccaccta tccacccttt gaatctatag gtgctaataa
    17041 tgagattgtc ggctttgata tcgatctggc aaaagccttg tgcaaacaaa tgcaggcaga
    17101 atgtactttt acgaatcacg cgttcgacag cctgatcccg tcactgaaat tcagaaaata
    17161 tgacgccgta atctccggta tggatatcac cccggagcgt agcaaacagg tatcgtttac
    17221 cacgccctac tatgaaaact cagccgtcgt gattgccaaa aaagatacct acaaaacgtt
    17281 tgccgatctg aaaggcaaac gtattgggat ggaaaacggc accacgcacc agaaatatat
    17341 tcaggagcag cacccggaag tgaaaactgt ctcctatgac agttatcaga atgcctttat
    17401 cgacctgaaa aatggtcgta ttgatggggt atttggtgac acagcggtgg taaacgaatg
    17461 gctgaaaacc aatccacaac tgggcgttgc gactgagaaa gtgacagatc cgcagtactt
    17521 cggcaccggc ctgggtatcg ctgtacgtcc ggataacaaa gctctgctgg agaaactgaa
    17581 taacgcgctg gcggcaatta aagctgacgg tacatatcag aaaatcaatg accagtggtt
    17641 cccacagtaa gattcagaaa gccgcacctg cggctttttt attgcttcgc cagcagcgtc
    17701 agcacttcat agtgcgcggt gtgcgggaac atatcaaaaa gctgtacccg ttcaatacgg
    17761 taacctggca gttcgcggat atctttcgcc atggtttggg cgttacagct ggagtagata
    17821 ataaaacgcg gtgccatcgt tgagagataa tcacacagcg gtttaccaat gccacgtcgc
    17881 ggcgggttaa ccagcaccag ctcaggcact tccccctggg cggtggcaaa ctgagtggag
    17941 tccagcgcct gaaattgcaa acgcgttaag cccagttcag cggctgactg cttcgcacag
    18001 gcaatggcct ctggtgcaat ttcgatcccg gttaactgca tgtcaggcgt cgcgcagtgt
    18061 aaaccaaagc cccccacgcc acagaacaga tcccacatat gtttaactgg cagctgccgt
    18121 acccagtcgc gcgcggtagc gtacagctgg ctggcgaccg ccggattagt ctggaagaaa
    18181 ctttgcggac ggatccacag cggtacgtca ttaaaacgct ctgccagcgc ctgttgttca
    18241 gtcaggtaga tctccgtctc cccttccata atcgccatat gtaccggctg aatattgacg
    18301 gtaataactt tcagctgcgg tagttgttcc tgtaaccacg gcagcgcctt acgcagttgc
    18361 gccagcttgg tatcagaacg cagtacaaag cgcagcatca tgccgccatc gctctggctt
    18421 tcagtcagca gaatgtattt cagttcgcca cgcttacgcg ccacgttgta gggcgttaac
    18481 cccgctcggg cgataaacgg ttttagcgcc gcaaaaacgg gcgcaaatga ggcaggataa
    18541 agcgggcagt cacaaaggtc ttccggtgtg ccgtctcgat gcagcatacc gagcagtggt
    18601 ttttcaacgc taccactcac caccattttg gctttattac gaaacccttg ttccgggcct
    18661 gacactggcg cacaccattc ctcaaccgga aagtcggcga gcagattttt aagatcggcg
    18721 gttttagcgg agagttgctc tggaatcggc tgcgttatcc actgacagga acgacagcga
    18781 cccgcgtcgt aaagtgcgca ctgcatacat tgaccttcac atcatcaggg gcgacgatta
    18841 tacacattat tgcaactgga agaaccgccg actggtcgag ggaacgaaca gtagcaacag
    18901 gatgagcata tccggcaact tctgcagcat caggctatgg aagatttcac gttttgattc
    18961 accgggaatg ctgaacagct ccggataacc ataccccagc gaagctgccc acaagtaact
    19021 tgcggctgtg atttgtgtca acagatacag ccagcgcgcc cagcgacgcc ctttaaccag
    19081 cgaaaacgca caccagatct caatgaaaac cagcaccaga ctgctcaaaa agaccagcgt
    19141 taaactccag gtttgtacgc tgcgatgaat gaactcgcca ataccgcgca cgcccagcgt
    19201 attgaaaatc atcagcacgt cgaggccacg gatcataata atggcgagcg ccgccacctg
    19261 caccagcgca ggcacattca ggcgagcatg agatgaagat gttttcttaa aaaatcccaa
    19321 cgtttcgtct tccatgaaaa caatgccgcg acatgcgcgg cattatgtag ccaggttggc
    19381 aaattttagt gtcttcagcc acgtcttgca cgctggatgt cgcgtatccg ctgtttttct
    19441 gcgcgcgcca tcagatacca ggcgataaat ccgacaattc cgaccgcgcc gaggatcaat
    19501 gttgccaggg cgttgatttc cggattcacc cccatccgca cgctggaaaa gaccagcatc
    19561 ggtaacgtgg tggctcccgg cccggaaaca aagctggcga tcaccagatc atcaagcgac
    19621 aaagtaaaag ccagtaacca gccagaaatg atcgcgggca tgatcatcgg tagcgtaatg
    19681 acgaaaaaca ctttcaacgg cgtcgcaccg agatccatcg ctgcttcttc tatcgagcga
    19741 tccagttccc gcagacgcga cgaaataacg actgccacat aagccgtaca gaaagtgaca
    19801 tgcgccagcc agatggtgaa cataccgcgg tccgcaggcc agccaatggc atgagcaagc
    19861 gcgacgaata acaacaatag cgacaagccc gtgatgacat ctggcatcac cagcggcgcg
    19921 gtgatcataa aggcaaaacc atttgatccg cgaaacctgc caaagcgcac cagcacgacc
    19981 gccgcaatcg tcccgaggat cgccgccgcc gtcgccgcac aggccgcaat tgtcaggctt
    20041 aaaccaaccg cactcatcat tgcgtcatcg cgcaataact caccatacca gcgtgttgac
    20101 cagccggccc acaccgtcac cagcttcgag ctgttgaatg aatagatcac cagcatcagc
    20161 attggcgcgt agagaaaggt gaagcccagc agcaaaatca caatccgcca gggcgaacga
    20221 actaccggta aattattcat ccgtgttctc ccacgctttt ttgctgatgc ttgtggaacc
    20281 acattatcgg cacaattagc agcagcaaca tgatgatcgc taccgccgag gccaccggcc
    20341 agtcgcggtt gttaaagaac tcctgccaca gcacgcgccc gatcatgatg ctgtccgggc
    20401 caccgagcag ttcagggatc acaaactcgc ccacagccgg gataaacacc agcatcgatc
    20461 cggcaataat cccgcctttg gtcagcggca cgatcacagt aaagaacgtt ttcagcggtc
    20521 gtgcaccgag atccagtgcc gcttccacca gcgaataatc aatacgaatc aaggcggtgt
    20581 agatcggcag aaccataaac ggcacgtagg cataaacaat gccgatataa acggccagat
    20641 tggtatgcag aatggtcagc ggctggtcga taacccccag ccacagcaga aaattattca
    20701 gcacgccgtt attttttaat attcccatcc aggcataaac gcgaatcaga aacgaggtcc
    20761 acgacggcag gatcaccagt agcaataaga tattacgggt cgaaggctta ctgtgcgcca
    20821 ccgcccacgc cagcggatag ccgatcagta aacagcaaat agtcgaaatc gccgccacct
    20881 gtagcgactg gagataagca tcgaaataga gcggatcgtc ggtcagttgc aggaaattac
    20941 cgagattaag agtgatggaa agttgcccgt cagcccactc catcagttcg gtataaggtg
    21001 gaatggcgcg cgccatctcc gccaggctta ttttaaagac aatcagaaat ggcagcagaa
    21061 acagcaggat tagccagata tacggcagcg caatcaccag tttgcgccca tgcttcattt
    21121 gcaactgcga cagccacagc ttaaagggtt ttggctgggc agcaggttca agtgtactca
    21181 tctttgctcc ttaaaccgtc agcaccacac agctgtccac ttcccagcac aaacgcactt
    21241 catcgcccca ggtcggtaac cctttacgat gacgatgggc gttttgtagc tgggcgctaa
    21301 tcatctgccc acttttcaga cgaacgtgat acaccgaaag atcgccgaga taggcaatgt
    21361 gtatcacctc ccccaccgcg aagttacaac cattggcggg cggctcttcg caaagcatga
    21421 ttttttccgg acgcagcgct acgtgtaccg gcacgttatc gaccaccgaa gcatctacat
    21481 cgactttcag tggatgcacc agccccggcg aataaagcac cagaccatct tcctggcgct
    21541 ctttgagtac acattcaaag acattaaccg agccaataaa ttcggcgctg tagcgggtgg
    21601 tcggatgctc atagatctct tccggttcgc caatctggac aaatttccca cgattcataa
    21661 tggcgatgcg ccccgccatg gtcatcgcct cttcctgatc gtgggtgacc atcacacaag
    21721 tcacaccgac acgctccaga atatccacca cttcaagctg catccggtca cgcagctttt
    21781 tatccagcgc gcccataggc tcatcgagca gtaatagttt cgggcgtttg gcaaggcttc
    21841 gggccagggc cacacgctgt cgctgaccac cggaaagctg atgcggtttg cgtttggcga
    21901 actcctgcat atgcaccagg ccgagcatct cattgacccg gctggcaatt tccgctttcg
    21961 gcaatttgtc ctgtttcagg ccaaaagcga tattctgttc cacggtcata tgcggaaaca
    22021 gcgcgtaaga ctgaaacatc atattgatgg ggcgcaggta aggcggaacc tgtgacaaat
    22081 cgacgccatc gagcataatc tgcccggcag aaggttgttc gaaacctgcc agcatacgca
    22141 gcagcgtgga cttgccacag ccggatgcgc ccagcagcgc gaagatttcg cctttataga
    22201 tggtcaggct gacatcatcg accgcatgct gaccatcgta ggatttggtg aggttgcgga
    22261 tttctaatag cggcgtcagc gccttacggg ttttcgcctg cgggcgaggg atagcgtcat
    22321 tcacggcagc tctccggcat tgaatgtgtg caagcgcacc ataatggtgc gaattgccgg
    22381 cgggtgtggt gcgcccgtcc ggcatctacg actgcggatt attttccgct cttcactttg
    22441 gtccacgcgc gggtgcgcac acggtcgatt ttcggatcct gtactttcag agtgaacagc
    22501 ttcgcacgaa catccgcagg cgggtaaata cccgggttat cacggacttc cgcactcacc
    22561 agcggcgtag ctgctttatt ggcgttagca tagaacacat ggtcggaaat atgcgccacc
    22621 acatccgggc gcagcaggta attcaggaac tgataggctt cgtctttatt tttggcatcc
    22681 gcaggcatgg cgaagacatc aaagaacgcc atcgcccctt ctttcggaat cgagaacgag
    22741 acattaacgc cattcttcgc ttccttcgcg cggtttgacg cctgccagac atcgcccgcc
    22801 cagccaatag cgacgcagat atcgccgttt gccaggtcgt taatgtattg cgatgaatgg
    22861 aaataacgaa tgttcgggcg cagctttaac agcagatcgg ttgccggtcc ggtgtaatca
    22921 tctgctttag tgctgttggg atctttaccg agataattca gcacggtagc aaacacttct
    22981 tccggtgcat caaggaaaga gacgccgcag cttttcagtt tttccagatt ttcaggcttg
    23041 aggatcaagt cccagctatc gacgggcgcg ttttcgccca gcaccgcttt aactttatca
    23101 acgttatagc caatcccggt cgtcgcccac atatagggca tagcaaattt attatccggg
    23161 tcgtgtttgg caaccaactt cagcagttcc gggtcgagat tcttccactc cggcaattta
    23221 cttttatcca gcggctggaa aactcccgca gtcaactggc gttcaagaaa gcttgctgac
    23281 ggcaccacca ggtcaaagcc ggtgctaccc gccattaatt tgccttccag tacttcgtta
    23341 gagtcgaaaa catcgtagac gactttaata ccggtttctt tttcaaaatt ggccaccgtg
    23401 tccggggcga tataatcaga ccagttataa atgtggagtg ttttttgttc agccgcgagc
    23461 gtgccgacag agacggccat cagagcaccc gcaaccagac ccgatagcca ttttttattt
    23521 aaggcggtca tatctctttc cttctgaaag ttcgttaaca aacgaacaaa agttgtcaca
    23581 ctgaaagata tgcaataaac gtgcatattt tttatcggcg cggtgtaaga aaagagaact
    23641 ctctcccggc cagcactgct cgctatataa aagcatcgca agaataactt tagccagggt
    23701 ttgagactat agctcggatt aaaaaacggt atttaacaaa tttttatgcg tatttgctct
    23761 atgtgataaa gccaaatcgg cacaagagaa gtgaaataat ctttaagaaa aggttaaaaa
    23821 cagcagataa ttttgcagca cgcagtcact gcggcagcga ctgcgtactc gaaaggagtt
    23881 atcagtggag gaaatagttt tcggtaacgt tgttttgccc ttcatcgtca gtcggtaaca
    23941 gcagttgatg ggcattagct tcaagaatga ccatcgaaat ctgctcttcg ctctgacgca
    24001 cgaaccatgc aaactgctca taggttacgc cctgcataac ggataaagac tggcaaacca
    24061 ccagctttgg cagattatca tcctgcatat caagaaatgc tttcacggtc agcgaactgg
    24121 cattgatggc agataaatct gccgccaggg gcaatacggc tgaaggtcgg acttccgcca
    24181 tggcagaaaa caggatcgtg ttatcgatca gatcgatttt ggcatcaaag acaccgtcga
    24241 aattctgcat atggggcaga tgcagagcct gacagttatc acattcaaaa aaactcatcc
    24301 ccaggtcatc gagccattga cgcagcgtat ccagaccagg aacgaccagc gatgtcataa
    24361 ttttgtacaa cctctttcga taaaaagacc ggcacagctt acgcaaaaag cgcaggcaaa
    24421 accatgatca gtaatgtgat tgcgactaac cacccgtttt caggcaatat tctgtcgtag
    24481 cgtggcgttc gatccagcgg atcattttac ccgcgatgtc gataccggtg gttttttcta
    24541 ttccttccag ccccggcgac gcattcactt ccatcaccaa cggcccgcga ttagcacgca
    24601 gaatatccac accagcaacg tccagcgcca tcgttcgcgc ggcttttatc gcgatttcac
    24661 gctcctgtgg tgtgatactg gcgacgcttg ccgcgccgcc acgatgcaaa ttggaacgaa
    24721 aatcgccctc tttcgcccgc cgttcaatcg cagcgaccac ttcatcgcca acaaccagac
    24781 agcggatatc gcacccttgc gcctctttga tatattcctg caccagaata tgcgcgttca
    24841 gaccgcggaa agcgtcaatc acgctttcgg ccgcctgacg cgtctccgcc agcacgacgc
    24901 caattccctg cgtgccttca accaacttga ccaccagcgg cgcaccaccg accatgtcga
    24961 ttaaatcgct ggtatcatcc ggcgaatgcg caatgcccgt gacaggcagg tcgatgccct
    25021 gacgcgccag cagttgcatg gaacgcaatt tgtcacgcgc ccgggcaatg gcgaccgact
    25081 cattgagcgg atagctcccc agcatctcga actggcgcag tgccgccgtc ccataaaagg
    25141 taatggcggt gccaatacgc gggatcactg cgtcaaaatg gggtaacttg cggcctttgt
    25201 agtgaataga agacgccgca ggatttatgt tcatgtagca agaaagcgga tcaagaattt
    25261 caaccaggtg accgcgctgt atagcggctt cacgcagccg cttacacgaa tagagcgttc
    25321 catcccggga caatatggca attttcaccc tgcacctctc tgtcagacct ggtcaaaagc
    25381 ccgcgtatca tacatcggcg tggcggtttt agcgcgtagc ccaaccctgt ttgtgcaaat
    25441 aatccagtat aaatgggcgg ctttctttaa tgattgttcg gcggatatga tcgctccagg
    25501 tatcccggcg attattgctg ccacgggtga ggtaatattc cgccagttgt tcgtcatact
    25561 gcgccagtgc gtctttatcc agcggttgat agttgttttc atgcaccaaa atggaggccg
    25621 gtaaacgcgg cttaagatcc ggattatccg caggccagcc aaggcacagc ccaaacagcg
    25681 gcagaacatg ctgcggtaat ttcagcagtt ccgtcaccgc ttcaatatta ttgcgcaggc
    25741 cgccgatata taccccgccc aatcccagcg attccgctgc ggttaacgca ttctgcgcca
    25801 tcattgccgt atcaacgaca ccgagcagca attgttccgc caggccgagc tgagcatccg
    25861 gacagatctg taaatggcgg ttaaagtcgg cacagaacac ccagaactcc gccgcttgcg
    25921 ctacgtgttt ttgcccgccg gtcagcgtca ccagttcatc acgtaacgct ttgtcggtaa
    25981 tgcgaataat gctactgcac tgcaaaaaac tggaactgga cgtcgcacgg gcgctgttaa
    26041 taatcgcctc acgctgcgct tcggaaatgg gttcatcagt gaaatggcga atggagcgat
    26101 ggccacaaat aagttcaatg gttggcgtca ttatcttttt ctctttctga acgtgaatat
    26161 tgcggtggac ggttcatcag ctgtggggca agacgttttg ccacctgaag aataaccacc
    26221 accgctgtgg gaagcatgag caaaacaccg agaaaaatca tcagaatctg cacttctggc
    26281 cgagaaaatg gctcaggcag cgacagggag ccgcttaccg acagcagcgc caccgccagc
    26341 aacatcattc cgataaattc cagtatcaac acgcttttag gcaatttacc gatcgcgcgc
    26401 atacgcttcc ctctgcaaag tgagccttca gtctaaaact tttcactgta ttgtgtttaa
    26461 cagttatagc ttttagcaat taatgcaaca ggttaaacct actttcagcg aatacatttt
    26521 agcgtgatca ttacaggcat aaatctatga ggagagaaat aatgcaaacc gttatttttg
    26581 gtcgtccggg ttgcccttac tgtgtgcgtg caaaagatct ggctgagaaa ttgagcaatg
    26641 aacgcgatga ttttcagtat cagtatgttg atattcgtgc ggaagggatc actaaagaag
    26701 atctacaaca aaaggcaggt aaacccgtag aaaccgtgcc gcagattttt gtcgatcagc
    26761 aacatatcgg cggctatacc gattttgctg catgggtgaa agagaatctg gacgcctgat
    26821 cgtctgacaa gccctcgcgt tgagggcttt attgattttt tctgtgctgt ggtttaaaca
    26881 aactactgat aaataagaaa cacagtgccc ccagcgcaca ccagaacacc gcacttaata
    26941 accatgccag ctcttgccag aatgagcgcg tcggtgaaaa aaacagccgc ataataagca
    27001 ttgaacaggg tgccgccagc attgcgccaa acagaggttt caggacttct ctacgctgtg
    27061 aaaagaagct ggcaactgct ccaggaagaa tgaaaaacag caagccgatt tcaggatgcc
    27121 cggcagcccg aaaagcgcct ttcatatgcg tcgccagaaa aaggcacacc acaatgaaga
    27181 ggacaaaaca gcagattgcc cccgcccaac gttgtttatg tttcactcgt tcctcctgac
    27241 actgcgtcta tcgaacacat ttttcgccag tgtggcgttc agtaagataa agccgcttcg
    27301 cattccatgc taatataggc caacgcaatt catatagccg ttgataccta atgtgattac
    27361 actagtaaaa tatattgtta ctttactatc gtttaggtgc gctgaatgaa tctgcgccct
    27421 gaattctggt aaaaaacatt atcgtaaatt accatttctt tcaacagctt actagtaaac
    27481 aagaagttag cctccgtgaa tataaacgtc gccgaattgt taaatgggaa ttacattctg
    27541 ttattatttg tggtcctcgc gcttgggcta tgtctcggga aattacgact tggttcgatc
    27601 caactgggta attccattgg cgttttagtc gtatcgctgt tattaggcca acaacatttc
    27661 agcattaaca ccgacgcgct taatcttggc tttatgctgt ttattttctg cgtcggggtc
    27721 gaagccggac cgaacttttt ttccattttt tttcgcgatg ggaaaaatta cctaatgtta
    27781 gcactggtga tggttggcag tgcgctggtg atcgccttag ggttaggtaa gctgtttggc
    27841 tgggatattg gcctgacggc cggtatgtta gcaggctcta tgacgtcgac accggttctg
    27901 gtcggtgctg gcgatacact gcgtcattcc ggcatggaaa gcaggcagct ctcactggca
    27961 ctggataatc tgagcctcgg gtatgcctta acctatttaa tcggtctggt gagtttgatt
    28021 gttggtgcgc gttacttgcc gaaattgcag catcaggact tacagaccag cgcccagcaa
    28081 atcgcccgcg aacgtggcct ggacacagat gccaaccgta aggtttattt gccggtgatc
    28141 cgcgcctacc gcgtcggccc ggagctggta gcctggaccg acggcaaaaa tctgcgtgaa
    28201 ctgggtattt atcgacaaac cggctgctac attgaacgta ttcggcgtaa cgggattctg
    28261 gcaaatccag acggtgatgc cgtactacaa atgggcgatg aaatagcgtt ggtaggctat
    28321 cctgacgccc acgcccgact cgatcccagt ttccgtaatg gcaaagaagt tttcgatcgt
    28381 gaccttctcg acatgcgtat cgtcactgaa gaagtggtcg ttaaaaacca taacgctgtt
    28441 ggcaaacgcc tggcacaact gaagttgacc gatcacggtt gcttccttaa ccgcgtcatt
    28501 cgtagccaga ttgagatgcc gattgatgac aacgtcgtgc ttaacaaagg tgacgtttta
    28561 caagtcagcg gcgatgcccg ccgggtaaaa accatcgccg atcgcatcgg ctttatctcg
    28621 attcacagcc aggtcactga cttgctggca ttctgcgcct tctttgttat tgggctgatg
    28681 atcgggatga tcaccttcca gttcagcaca ttcagtttcg gcatggggaa cgctgccggg
    28741 ttgttattcg ccggaattat gctgggcttt atgcgtgcta accacccgac cttcggttac
    28801 attccgcagg gtgcattaag catggtgaag gagttcggct tgatggtgtt tatggcaggc
    28861 gttggtctga gcgctggtag cggtattaat aacggcctgg gcgcgattgg cggtcagatg
    28921 ttgattgccg gattgattgt cagtctggtg ccagtggtta tctgcttctt gttcggtgct
    28981 tatgtattgc gaatgaaccg cgcactgttg ttcggcgcaa tgatgggcgc acgcacctgc
    29041 gcgccggcaa tggagatcat cagtgataca gctcgcagta atatcccggc gctgggctat
    29101 gcgggcactt acgcaatcgc caacgtcctg ctgacgctgg cagggacaat catcgtcatg
    29161 gtatggccag gattaggata aaactgaagt tgccctgaaa atgaaatttt tttgcacaac
    29221 cgcagaactt ttccgcaggg catcagtctt aattagtgcc actgcttttc tttgatgtcc
    29281 ccattttgtg gagcccatca accccgccat tttggttcaa ggttggtggt ttttttgttg
    29341 cctaaaattt atctactacc ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga
    29401 tagcggctta cggtcggtga caaaatgcag cgtcatcccc tcaataaacg catcaagcgc
    29461 gcgggcggtt ccgggttcaa accattgttc gagcgtttgc tgactgcgct gcatccagtt
    29521 ttgcattacc gtttttaata gcggttttcg gctagccagc gcgtagagct ggtacatcag
    29581 ctccatatta tccggcgttg caacctgtga gctgtagatc atatcggtga tagcctggca
    29641 tgcgcccgga gcatcactaa catcgctaaa aaatgcctga tattgccggg acatgatctc
    29701 agtaaaactg ctgaacgcct ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat
    29761 cgaccccaac ggtaccccgg caagggtagc aattttgcgg tgcgtaacag catgtattcc
    29821 gtaaagtttc accgcctcca gcgtggcctg gataattttt tctcgccgtt gcggatcgtt
    29881 agcgcgacgc atgtcatttc ctctctctgc aattgtgtac aaatgtacac aaccttgcta
    29941 atgttgtctt ccctcttctt attttgacgt gatctatgac cgtaaattct tcacgtaatg
    30001 cattgaaacg ccgaacctgg gcgctgttta tgttcttctt tttgccgggc ctgttaatgg
    30061 cgtcctgggc aacccgtacg cctgctatcc gcgatattct ttctgtttcg atcgctgaaa
    30121 tgggcggagt cctctttggt ctgtcgatcg gttcaatgag cggtattctc tgctcggcgt
    30181 ggttagtgaa acgctttggg acgcgtaatg tcatcttggt cacgatgtcc tgcgcattga
    30241 tcgggatgat gatactaagt ctggcactct ggctgacatc gcccctgctc ttcgcggttg
    30301 gtctcggcgt ctttggggca agttttggtt ctgcggaagt ggcgataaac gttgaaggtg
    30361 ctgccgttga gcgagaaatg aataagacgg ttttgccgat gatgcacggt ttttatagcc
    30421 tgggcacgct ggcaggcgct ggtgtcggga tggcactaac ggcctttggc gttccggcaa
    30481 cggtgcacat tttattggcg gcgctggtag gcatcgcgcc tatttatatc gccattcagg
    30541 caatccctga tggtacgggc aaaaatgctg ccgatggcac ccagcatggt gaaaaaggcg
    30601 taccttttta tcgcgatatc cagttgctac tgattggtgt tgtggtgctg gcgatggcct
    30661 ttgccgaagg ttctgccaac gactggttac ccttattaat ggttgacggt cacggtttta
    30721 gccctacttc cggctcgctg atttatgccg gttttaccct ggggatgacc gttggacgct
    30781 tcactggcgg ttggttcatc gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac
    30841 taatgggggc gttaggtatt gggctgatta tttttgtcga tagcgcctgg gtcgctgggg
    30901 tgtctgttgt actctgggga ctgggtgcct cgctgggctt cccactgacc atttctgccg
    30961 ccagcgatac cggccccgat gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc
    31021 tggctttcct cgtcgggccg ccgctgctgg gctatctcgg cgaacattat ggattacgta
    31081 gtgcaatgct ggttgtactg gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg
    31141 ccaaacccga taccaaaacg cagacggcga tggagaatag ttgatgagca ttaaattaat
    31201 tgcggtagac atggatggta ctttcttaag cgatcaaaaa acctataacc gtgagcggtt
    31261 tatggctcag tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa
    31321 tcaatattat cagttgatct ctttcttccc tgaaattgct aatgaaattg cctttgtggc
    31381 tgaaaacggc ggctgggtag tgagcgaagg caaagatgtt tttaatggcg agctgtcgaa
    31441 ggatgcgttt gctactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc
    31501 ctgcggaaaa aatagcgcct ataccctcaa aaaatatgac gatgccatga aaacggtggc
    31561 ggaaatgtat tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatctt
    31621 ctttaagttt ggtctgaatc tttccgatga actgattcca caagtacaaa aagcattaca
    31681 tgaggccatc ggcgatatta tggtgccggt ccacaccggc aacggcagca tagatctgat
    31741 tatccccggc gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga
    31801 cgacagcgaa gtagtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc
    31861 aggctttagt tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg
    31921 ggcaggctcc aataaccgtg aaggcgtact ggatgtgatc gataaagtgc ttaaacacga
    31981 agcgccattt aaccaataaa acggcagcac gcccggcaac acaatgcctg aatgaagatt
    32041 gcataattcg cggaaattaa cccgctgcat tatttcaggc gaaatgatta tgaaaaattg
    32101 tctactgttg ggcgcgcttt taatgggctt tactggtgtg gcgatggcgc aaagtgtcac
    32161 cgtcgatgtg ccgagtggat acaaagtggt tgtggttcct gattcagtca gcgttccaca
    32221 agcggtcagc gtcgcgactg tgccgcaaac cgtctatgtg gctcctgcgc cagctccggc
    32281 ttaccgcccg catccttatg ttcgccatct tgccagcgta ggtgaaggga tggttatcga
    32341 acaccagatt gacgaccatc accattaatt cgagaatgcc tgatcgcaca aagcaatcag
    32401 gcattttttt acccttcgtg cgaatttccc atctgtttat cttttaaaaa gataaccatc
    32461 agcaacagcc acaaaattcc gttgacaagg ttgaagagat taaacagtcc gttgcccccg
    32521 ttcagccagg catgtttgct gatttcaata ccgacggtaa agatcagcat ttgcagcatc
    32581 cccatcgccg cagaaaccgt acctttactc atatcgctgg caaacagagt taatcgcacc
    32641 agtcccgcat tcgccagacc aataccgaaa gcataaatac ttaacccggc ggtcatccat
    32701 aaatacgcgt gcgatgagat aaccgttgcc gcagcagcga caaacagacc aatcataatc
    32761 ggccagccgc ccataataat cagcgaacgt acggtgcggc gcgaggtcag acgcgctagc
    32821 agcaagttac ccgcaattaa cgccccgaaa ataggcactt gcagcaagcc atattcatag
    32881 ctgctcaact gctcaccggt aatgatgata atcggcgact gggcgatcca cgccagcaac
    32941 ggcaggctaa cgaatcccag cgccagcgcc cccgccacaa agcgaccgtt cttcagcacc
    33001 agcttatagt cacgaccgag ttctttcagc gacagtttct cgcctatacg cgtggcggtt
    33061 tcaggcatgg ctcgttgcag accgaaaaag gagatcgctg ccaatgcggc aaacaaaaca
    33121 aacatcccct cccagggcag cacatggatc cacgccgcgc ccaccagcgg accaagtagt
    33181 ggagcaatca gcgccacgtt cgccattagc gcggtgatct tgatacaaac cgcctcttcg
    33241 aaggattcct gaattgcggc atatcccaca gcgccaatga aacagaggct tatgccctgc
    33301 aagaagcgca acagggtgaa ttgttcaatg ttttgcgcca gtaatattgc cagacaggtg
    33361 acgataaacc acaccactcc cgccagcatc accggacggc ggccaatacg atccgacagt
    33421 ggccccagga gccattgtaa aaacatcccg cccgccagat acgcagtcat cgaagtagga
    33481 acccaatcaa tgcccgcctg atattgttcc accacggcca acataccggg ttgaatcata
    33541 tcgttgccga tataggttga aaattcgtaa agcaccagac agagagggaa aagtaacgcc
    33601 tgacgtccaa gcctggcacc ggaagctaat ttattttgca tgcaatttct tcgccaataa
    33661 taatcgcgca aagtttaata aaagcgcagc taacgagaaa gcgaattttg tagctgaaac
    33721 cacggttaag cacattctta cattatcgcg agtatagcta cgctttcttt aagttttatt
    33781 taacctatgc ccgttacaat cacccaccgt aaacaggccg cttgagggaa ataagacgat
    33841 gccgctttac ccagtttaac ctgcacttta ttctcaacga cttgcctgta ttggctccct
    33901 tttaatcact ttgcgtcggg aagttatgct ggaaaatttg aatctctctc tattctctct
    33961 cattaacgcg acgccagact ctgcgccgtg gatgatctcg ttggcgattt ttattgctaa
    34021 agatttgatt accgtggtgc cgctgctggc tgcggtactt tggctgtggg ggcttacagc
    34081 acaacggcaa ctggtgataa aaatcgctat cgcgctggcg gtcagcctgt ttgtgtcctg
    34141 gacgatgggg catctttttc cgcacgaccg accctttgtc gaaaatatcg gctataactt
    34201 cctgcatcat gcggcggatg actcattccc gagcgatcac ggtacggtga ttttcacctt
    34261 tgcactggca tttttatgct ggcatcgcct gtggtccggc tcacttttaa tggtgctggc
    34321 cgtcgtcatt gcctggtcgc gcgtttatct tggcgtccac tggccgctgg atatgctcgg
    34381 tggattgctg gcaggtatga ttggctgcct tagcgcccag attatctggc aagcgatggg
    34441 gcataaactc tatcaacgtc tgcaatcgtg gtatcgcttc tgttttgcat taccgatccg
    34501 caaaggctgg gtgcgtgact gatctctgct aaaaagtgta gtattgagcg gctcgcttca
    34561 ataactattc agagggatta tggaaacacg tcgcgaagag cgtatcgggc agctgctgca
    34621 agaattaaaa cgcagcgata agttacatct taaagacgcc gccgccctgc ttggggtttc
    34681 ggagatgacg attcgtcgcg atctgaacaa ccacagtgcg cccgtcgttt tgctcggcgg
    34741 ctatattgtt ctggaaccgc gcagcgccag ccattacctg ttaagcgatc aaaaatcccg
    34801 cctggtggaa gaaaaacgcc gggcggcaaa actggctgcg acgctggtag aacccgatca
    34861 gaccctcttt tttgactgtg gcaccaccac accgtggatt attgaagcga ttgataatga
    34921 aatccctttc accgccgttt gttattcgct aaataccttt ctggcactga aagagaaacc
    34981 ccattgccgc gcgtttcttt gcggtggtga atttcacgcc agcaacgcca ttttcaaacc
    35041 catcgatttt cagcaaacgc tgaataattt ttgcccggat atcgcttttt attccgctgc
    35101 gggcgtgcat gtcagtaaag gcgctacctg ctttaatctt gaagagttgc cggtaaaaca
    35161 ctgggccatg tcgatggcgc aaaagcatgt gctggttgtc gaccacagta aatttggcaa
    35221 ggtgcgtccg gcgcgcatgg gtgacctgaa acgctttgat attgttgtga gcgattgttg
    35281 cccggaagat gagtatgtga agtacgcgca gacgcagcgt attaagttga tgtattaaca
    35341 actaataacc ggatgacgtt tcgcgccatc cggctatcag aagattaaga gaaccagctg
    35401 ccgaaccact gatggaattt catcatcacg aaatcccaca tccgaccaaa gaatccgccc
    35461 tcttccacat tttccatcac gatcagcgga cgctgctcaa tggatttacc gttaagctgg
    35521 aaatcaatgg tgccaacaac ctgacctttt ttcagcggtg cggtaagctg cggttccgtt
    35581 aacgtgtagc tcgctttcag gtttttcagc tgcccacgcg gtatggtcac tgagcccgct
    35641 tcgcctgccc cgagattcac ttcgctctta tcaccaaacc agacgcgctg agtcacaaag
    35701 gtggcatcag gtttaattgg cgtcacggtt tcaaagaagc ggaaacccca ggtcaataat
    35761 ttctcagact cattaaaacg gatacggtca gttttcgcac ccagtactac ggagatcaaa
    35821 cgcatatcgc cctgggtagc cgaagcaacc agattatatc ccgcgcctgc cgtggttcct
    35881 gtcttcatgc catcaacatt cagattgctg ctccatagca gacggttacg gttaggctgt
    35941 cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg cgtactcttc cggcacatcg
    36001 tggattaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg ccccggcgca
    36061 tccagaccgt gcaccgtctg gaaggtagtg ttggtcagac ccaatttttt ggcataacca
    36121 ttcatcagac caataaatga ctcctggctc ccggcaacgt aatcggccag cgcaatacag
    36181 gcgtcattac cggactggat aatcacacct ttgttcaggt ctgccaccga aacctgatcg
    36241 cccggtttaa ggaacattac cgatgaacca cgcagtgccg gatttcccgt cgcccaggca
    36301 tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag cgcctgccca
    36361 accacatagc tggtcatgat tttagtcagg ctcgcggggt ccagtttttc atccgcattg
    36421 ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg cgcatccacg
    36481 ctcggcgctt caacggtttg ttccgccgcg aatgccgttg gggcaaaaag gaataaaaat
    36541 gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa tgccacccga
    36601 gtatccattc aaaaaaaata cgttatatcg ccgtatgata agttcggtga ttaataacgc
    36661 acacacaaac ttaaagaaac cgtaagttgg taaagttttt aaagtttatg caatatcctg
    36721 ccgcttcgct gcttctggtg tcattttttt gccagctgtt gcttaaaagt taactttact
    36781 tcaccatcgg agtcctgttc agcctgtggc tgcctgtcgg aaaaggggtg agtatgatta
    36841 cgctgtgggg tcggaataat tcaaccaacg taaaaaaagt attgctgacg ctcgaagaac
    36901 tggaactacc ttatgagcaa attctcgcgg gccgtgagtt tgggataaat cacgatgctg
    36961 attttctggc gatgaaccct aacgggctgg tgccgttgtt acgcgacgac gaaagtgatc
    37021 tcattctttg ggaatcaaac gccattgtcc gctatctggc ggcacagtac gggcaaaaac
    37081 gcctgtggat cgactcaccg gcacgtcgtg cggaagcaga aaaatggatg gactgggcaa
    37141 accagacgct cagtaatgct catcgcggga tcctgatggg attagtcaga acaccaccgg
    37201 aagagcgcga tcaggccgcc attgatgcca gttgtaaaga gtgcgacgcc ctgtttgccc
    37261 tgctcgatgc ggaactggca aaagtaaaat ggttctctgg cgacgagttt ggtgtgggcg
    37321 atatcgctat cgctccgttc atctacaatt tgtttaacgt tggcctgacc tggacaccgc
    37381 gtccaaatct gcaacgctgg tatcagcaac tcactgaacg ccccgcagtg cgcaaagtgg
    37441 tgatgattcc cgttagctaa ttgcgtgggc taactttaag taattcccca ctggactcgt
    37501 cggtgagaac gtataaataa ccgtcgggtc cagtgcgaac atcacgaatt cgctgccctc
    37561 tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc gttgacgctc atcacaatga
    37621 catctttatc tttcagcgcg ccaataaata atttttgctg ccactggggg aatttatcgc
    37681 tgttatagaa ggccatgccg ctcacggcgg gcgaatcttt ccagtaaaaa acaggttgct
    37741 cggtcccggc gacgatctcc cctttcgctt ccggtatctt aaagcctgaa tagttgattc
    37801 cccaggttgc cagcggccag ccgtagtttt tgcctttttg cgggatatta atttcatcgc
    37861 caccgcgcgg gccatgttca ttcagccata gtgcattact ccacggattc atcgccattc
    37921 cttgcggatt acgaatgcca taagaccaga tctcggcgcg cgcaccggat tcctttataa
    37981 aaggattatc atccgggatt tcgccctggt cggtcagacg caccagtttg ccctgtaatt
    38041 tattcagatc ctgcgccgtc gggcgctgat tgttttcgcc cagagcaata aaaagataac
    38101 ctttaccgtc gaataccagc cgcccgccaa aatggttgcc ggtagacagt tttggcatct
    38161 ggcgaaagac ggtgcggaag tcggtcacct ttgagagatc atcacttaag cggccataac
    38221 ccacagcagt tccggctttg ccatcatcgc caacttcgga ataacttaac cagatgcggc
    38281 gagactgagc aaaatcaggc gctaaaacca catccagcag gccaccctgc ccgtgcgccc
    38341 aaacttccgg cactccggaa agcggcgcag ataatccttt tcctgcttgc cagtgacgca
    38401 actcgccgcc gcgcagagtg attaacatac cgtgattgtc gggtaaaaag gccagcgccc
    38461 agggatggtc gagtttgtcc tgcagtactt cgacatttac cgttgcagga gccgcccaga
    38521 gagcggaaga aagacaaata aggggcacaa ggaaaaagga ttgtcgatgc ataacgcctc
    38581 cggattgact ctggcttaaa gcgtagtcag tggaggagat aacaaattca tttttacaaa
    38641 aacttaaaca tgaaggggga gacgctttct cccccttagt tttcaggcct tctcaagcat
    38701 ggcgtgcttc tgcaggctct ggatactcag cgttaagctc atcagacaat tttcaagctt
    38761 atcggcgttg acggtaataa cagtcgggca atcatggtgc ccactcatca aacatactgc
    38821 ggctgtcgct aatgcttctt cagcatgatg aagagcactc cactcttcct gatccagatg
    38881 aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc agtttaaaaa agttatctcg
    38941 tagatgattg ctttcgctga cggacatgta tccttttgcc ttcctcagtt gcacgtatgc
    39001 ggcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga tcgattcgct gtctgtcaac
    39061 gaacatactt cgatcctcct ctttccagcc ttgttgcaat taaatagtat ggctattttt
    39121 tgagcaaaat gctgtccagg atcaattctt cacgccgaat taacaaaatt tacatttcct
    39181 tcgcctccgc cagcgaatga gattgcgata caaattttat ccagaactct gtagaatccc
    39241 tgccctgacc tttcctgtaa ctgaatattt tttaagctat gagcaaagta actccccagc
    39301 cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct tgttgattca gagcgtattc
    39361 tcaccgaact acgcactgaa ggttatgacg tggtaccgag ctatgacgat gcggacatgg
    39421 tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca agaatcactg gaagccattg
    39481 gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg ttgtctgggg gcaaaagaag
    39541 atcagatccg cgaagtccac ccgaaagtgc tggaaatcac cgggcctcat agctatgagc
    39601 aggttctgga gcacgttcat cactacgtgc caaaaccgaa acacaaccca ttcctgagcc
    39661 tggtgccaga acaaggcgtg aaactgacgc cgcgtcatta tgcctatctg aaaatttctg
    39721 aaggctgtaa tcaccgctgc accttctgca ttattccgtc tatgcgcggc gacctggtga
    39781 gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct ggtagatgct ggcgttaaag
    39841 agattctggt gatctcgcag gatacttccg cctatggcgt tgatgttaaa catcgtactg
    39901 gcttccacaa cggcgagccg gtaaaaacca gcatggtcag cctgtgcgaa cagttatcga
    39961 aactggggat ctggacacgt ctgcactacg tttaccctta tccgcatgtg gacgacgtca
    40021 tcccactgat ggcagaaggc aaaatcctgc cgtatctgga cattccgttg cagcacgcca
    40081 gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt agatcgccaa ctggcgcgca
    40141 tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg ctcaaccttt attgtcggct
    40201 tccctggcga gacggaagaa gatttccaga tgctactcga cttcctgaaa gaagcgcgtc
    40261 tggatcgcgt tggctgcttt aaatacagcc cggttgaagg tgcagacgcc aatgccctgc
    40321 ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa ccgtttcatg cagctgcagc
    40381 agcagatttc cgccgagcgc ctgcaagaga aagtgggccg tgaaattctg gtgattatcg
    40441 acgaagtgga cgaagaaggc gcgattggtc gcagcatggc agatgcaccg gaaatcgacg
    40501 gcgcggttta tctcaatggt gaaaccaacg ttaagccggg tgatatcctg cgtgtgaaag
    40561 tcgagcacgc cgatgagtac gatttgtggg gtagccgggt ttaattaatc ttaaggcaga
    40621 tctaacaagg atattgttct tcgatctgcc tctgctgccc ttcccgatgt cctaccctta
    40681 cgcacaaaat cccctgacgc ataaatcgag aaaaatgaat cgtactcgag gcaactctca
    40741 ctttttcaca ccaaattaaa gttttgatcg caattacttg aaataagcgt aatggttact
    40801 atagtaaaca ttaccacaag gatggatgta tgggtgtgta taaggcaaga cgattcagcc
    40861 aaagcacgaa aaaacttggt attcacgata aagttctcat ggctgccgct gaagaagtga
    40921 tgcagggcat atgggaggct gatttaggta gcggtgtcat caaaaaacgt ttacccctcc
    40981 aacaaggaaa gagtggtgga gccagaacaa ttatcttttt taaatcagca aatcatgtgt
    41041 ttttttatga tggttggtcc aagagtggtc taagcagtaa aggaaccaaa gaaatcgaag
    41101 atgatgaact ggcggcctac aaaaaaatgg caaatgcatt tctggccttt agcaataagc
    41161 aaatagagga tttgatcgaa actggctttt tgattgaggt gaaaaatgag cgataaatta
    41221 caaatgatgc tggaagatgc aaaggaactg aatgcactgg gcctgatgtc tgatagtgaa
    41281 gttgatgcca ttgccgtcat ggtcaaagct cgtgaagtca gcgcccgtat agccaaagtc
    41341 aaatcaatga cgggtgaaga gatcaaagcc atccgcaccc gatacggcat gtcacaatcc
    41401 gtgctggcac atacaatggg aatgtcaaaa gaaagtgtct cgaaatggga acgtaatgaa
    41461 ataaaaccga gcggcccggc attacgtatt ttgaatacat tggcaataaa agggccggaa
    41521 gtttttgcaa agtaaaatgt ttgcaaatga tttaccacat tcattagtac cccttgatta
    41581 aggggtacta acactattct aattctactg ccagctaatc tgcgccctgc cgtttttctt
    41641 actcttatac agccgctcat ccgccagcat tttcaagcgt tcaacggtag tacattcatt
    41701 tgaatgcgct acaccgatac tggtgcttaa attaatgctt cccacctgga aatgaacaat
    41761 ttcttttaga atcgatagca actcttccag cgttccaccc gtataaatca ccgcaaattc
    41821 gtcaccgccg aagcggtaga ctttaccttt atctacgggg atctgtgatt taaggatttt
    41881 caccactttc atcaacactt catcgcccag caaatgaccg aaggtgtcgt tgatgttttt
    41941 gaatttatcg acgtcaaaca ggcacaaata accgcctttt tccggcagcg cttttaaatc
    42001 ttcgtcaaag gattttctgc ccattgcttt ggtcaggtca tccaggaacg ccgagttata
    42061 caaactgtag agttttttga catcggcagc tgaaaagaaa agcccgccat ccagtacgcg
    42121 ttcaaaatgg ttattaatga tggcttcttt ctccacaata ccacgattaa tcactctgcg
    42181 taccagatag aggcaactga cacccgatat cactgccacc aatgcggcaa agaaaataat
    42241 caatgtacag acccgcaaat aaacatcttt tttatacgca aatacgtaca atccggtcgg
    42301 caaataggcg aagtgctcca tcgcttccga aacggtgaag atggcgtttt tagcattata
    42361 ttcatcaccg gtcaccggtt taccgttctt aaacagagag aacatcaggt tctcgctatc
    42421 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
    42481 acgattatga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
    42541 ccctgtattt ttgacatgtc gatacttcac aatctgcata atatctgcac aataattttt
    42601 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
    42661 caaataagag ggtgaaaccg agtcggcgta agctgtgcgt aagccaacca acagctgttc
    42721 ctccgtggga tttagcgtgt tgataagatc aattgccacg ctacgccgat attcagcaat
    42781 atttgccacc gcaaaagcag tatcaataac ggactgtttc aggccataca cccgttcctt
    42841 taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa tcagcagact
    42901 gactgtaagt acgaatttat tgattctgga catacgtaaa ctactctttt actaattttc
    42961 cacttttatc ccaggcggag aatggcatgg gtttctggta cagatacccc tgagcctgaa
    43021 cacatcctaa tttgattaat tcacgcgcaa cgtcttcgct ctctacgcct tccgcaatca
    43081 ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt cccgcagtac
    43141 cgaaaatatc gccggtaaat tgtgagtcca cttttatgct atctggatta atttcataaa
    43201 aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatatccgt accccttgtt
    43261 tacgcagttg ttcaatattg agataaaaat gttcattaat tcgctcgatc acggtttcag
    43321 tgatctcaac acagaggctg ttcgccttca ggccattttc tttcatgatg gtggtaaaac
    43381 gctcatgaaa gtgtggttcg tttaactgcc agggcgaaac attaatatgc agtaagaaat
    43441 catccttcag cgacgcacgc tgactaaaat gtctgaattc acccattgcc gttttaataa
    43501 tctgataacc cagctcattg ataaacccga tatcttcagc aatggtaatg aactttagcg
    43561 gtgaaatgat cccccgctcc gcagacaccc aacggcacaa cgcctccgcg cccagaatgg
    43621 cgcgggtgtt aatatcaacg atcggttgat agacgaggaa tagttcgcca atttccattg
    43681 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
    43741 cacagaaaat aaactctgag aacatatggt cacggatact ggaaatcgcc agtttcagtt
    43801 tttctgcgta actccaggcg ttttctccct gcaaaggttc acaaatggca gacttatgga
    43861 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt tatcatcgct attttacggg
    43921 taaatgtctg tacattttcc cggcacagaa gtaaatagag atcggcattg aatgtcacga
    43981 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
    44041 tgcgttcatc aacaccaaac accgcttcca cggcttccag accacacagt ttgatgatcc
    44101 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
    44161 tttcctgatc gtaagagatc agtgagagca atttatttga cctttcaatt tcggcatctt
    44221 tcatttccgc gatcgaattt ttgatgctga taatctcttt cgcatcgcct tcgcgaattt
    44281 tcagagcctc gatctgatcc gttgagtcgg gcaaaaacgc cagcgtatta accagctcgg
    44341 tcatttcaga aaattgccga cggaacacac gagtggtcat taaataaacc accagcaata
    44401 cgataataat gaaagcaaac ggcagataaa aaagtttatc tgatgcgttc aataaattac
    44461 cgccaggata ataaaaatag atttcgaata atgcgttgtt tggcaccgct gatgtttttt
    44521 cgatatattc agtatcgaca gaatcaataa tattataatt accttcgcta ttataaataa
    44581 tcagtttttc acgcgcgagt tgattcttgg caatgatctc atggccttgt ttcaaaaata
    44641 gaacttcatc attagcgctg atttgatcaa gatactcgtt cagatatcgc cagtttaaat
    44701 cgacatgtat tttatccacc gctaacaaat cgtgatcttt gccgttgatg cgtagcgtaa
    44761 aagagataca ttttttaccc ggacgatcaa agcaattgta ggccggattc cagctgttgt
    44821 tagagtcatc tgaagccacc gaccatggtc gtttcgctgg attaaaatcg gcaaaaataa
    44881 cccgccctga ctcgtcgaca agcggtccat ttacttgttc ctgatgataa acaacgattt
    44941 tatcattcgc cctccgcgaa aagagataat atttattatc atcaagtacc agtcccaccg
    45001 agtctaaaaa tggcatcatt tccattttaa ggatcacagc gcgttgaatc tcctctgtat
    45061 tttttaataa atacagtgag tcctttaatg tcagggccga gcgttcaata tctttaaaga
    45121 aatagccaac gtttttctga ataagttcaa agcggctatc ggcacgtttt accgcgcgct
    45181 ccgataccag ttgatagtta atgatgaaaa aaagaccaat aaatgacagt gctgccaata
    45241 aaaataaaat tatcagcctt atctttatct tttcgtataa actcagcatg ttaatacatt
    45301 taacaaaata actatctgaa cgctgtgccg ctgcaaaaat tgtcccttgc tgcggctttt
    45361 taaccaaatt acccgattct acaaaatcag cagaaagtta caacacttgc ttagggcaat
    45421 aatttcctgt gaggtgatta ccctttcaag caatattcaa acgtaactat cctttaattt
    45481 tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt gaacgccagt acagtcagaa
    45541 aaatagccag ggccggaaaa atagcgacat gcggcgcgat aaccatatcc gctcgtgcct
    45601 cattgagcat tgctccccac tctggtgtcg gcggctgcgc accgaggccg agaaatgaca
    45661 ggctggcggc agagataatc gaggtgccaa tgcgcatggt gaaaaacacc acgatagaag
    45721 agacggtccc aggcaagata tgacgcaaca aaatggtcat atcgctggca ccaatactac
    45781 gtgctgactc gataaaggtt tgctgtttca acaccagcgt gttgccgcgc accagccggg
    45841 caaacgcggg gatggaaaaa atggcgactg cgataatcac gttagcaatg ccgcttccca
    45901 acaccgcaac aacagcgatc gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa
    45961 tgcgcatgat cagccgatcc caccagcctt cataatatcc agccaacaag cccagcaacg
    46021 tcccgatcgc cgcaccgata aacacggcaa acacgcccgc cgccagcgag atttgtgcac
    46081 caaccaggac acggctgaaa atgtcacgcc ccagtgaatc aacgccaaac cagtgctgca
    46141 aagaaggtcc gttattcaga ttgtcataat caaaataatt ttcggcgtca tagggggcga
    46201 tccagcgtgc aaaaatagcc accacaatca ataaaataac gaataatgcg gcggtcatcg
    46261 ccatatgctg acggcgaaat cgtcgccaga attcatgcca cggtgtacgt acctggtcag
    46321 gtttgaccag tggcatggcg tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc
    46381 ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc acatccacca ctaagttgat
    46441 aagaataaat tccagcgaga aaagcagaat ttccgcctga atcaccgggt aatcgcgcat
    46501 ttctacggag tcaacgagta agcgtccaag tccgggccag ttgaaaactt tctccaccac
    46561 gatggaacca ccgagcaaaa agccaaactg taagcccatc atggtcacaa ccgggatcat
    46621 cgcgttacgt agcccgtgtt tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc
    46681 ggtacgcata taatcttcgc ttaaaacatc gacaaacgac gcgcgggtaa agcgcgccat
    46741 cacggctgcc actgccgcgc cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc
    46801 tgctcccacg gtaggcagcc agcccagttc aacggagaat acctgaatta aaagcatccc
    46861 cagcgcaaat gccggaaacg agatccccga caccgcaatg gtcatactca atcgatccgg
    46921 ccaacgatta cgccagacgg cggcgataat tcccgccgcc ataccaaata tcaccgccca
    46981 gaccatactg gttatggtca gccacagcgt tggcataaag cggctggcaa tctcatcgcc
    47041 aaccggacga cgcgacacca tcgacaggcc aaaatccccc tgcacagcat tgctgatata
    47101 gtgccagaac tggtgataca gcggctgatc caaccccagc tgctgacgca ccagttctat
    47161 aacctgcgca tcagcttcgg gcccggcaat caatcgcgcc ggatcgccgg gcagcatatg
    47221 cacaaataaa aacaccagca ccgagacgat aaacagcgtt ggaatcaacc ccagtaagcg
    47281 tttgataacg taattaagca ttccactccc tgcgttgctt attgcaaatc cgcgtcttca
    47341 aagctgaaac cggtgtctgg catgatccaa aaaccggtca ggtttttact gtgtgccgac
    47401 accagttttt ctaccaccag cgggatccac ggcgactctt gccagatgat atcctgcgcc
    47461 gccttatata aacgggtctt ttccgccgga tcattagttt tcagtgcctg agccaggaag
    47521 tcatccacct gtttattgct gtaaaacgcg gtattaaaca gcgtcggtgg ccagttttgc
    47581 gatgcaaaca gcggcgatag tgcccagtcc gcttcgccgg ttgaagccga ccagccagtg
    47641 tagaacatcc gcacgccgct ctctttttgc cctttacctt caacttctgc cgcccgctgt
    47701 ccggcatcca tcgcagtcac ctgggtttta atcccgacct gcgctaactg ctgctgggta
    47761 aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg accacagcgt ggtattgaaa
    47821 ccgttgggat atcccgcctc tttcagtaat tcgcgcgctt tcactggatc gtaaggccac
    47881 ggtttataac tttgcgcgta ggcgatactc ggcggtacca caccagtagc tggcgttgca
    47941 tagcccgcga aggccacttt caccagcgcc gggcggttaa tggcgtaatt cagcgcctca
    48001 cggaccttcg ggttatcgaa cggcttttgc gtcacgttca tactgatata acgctgcata
    48061 attgacggac tggccatcaa ctcgatattt ttgtttttct ccagcagtgc ggcctgctcg
    48121 taaggaatgg ggaaagcaaa ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg
    48181 ttatccgcca ccggacgcca ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa
    48241 cccgcgaatt ttttcacctt cacaaaatcg gtctgattcc aggtatccag ttcatacggc
    48301 ccggttccca ccggatgaaa accaatctcc ttgccatatt tttccagcgc tgccggcgaa
    48361 atcatcgcgg tcgccggatg ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg
    48421 gtaatcttta ccgttgttgg atcgatcgct tccgttttgg caatattctt atacaggttg
    48481 tagcgtttaa gatgattcgc cggatcgctg gcccggtcca gattcgcttt taccgccgct
    48541 gcgttgaaat cggtgccatc ctggaattta attccttccc gcagtttcac ggtgtaagta
    48601 aggccgtcat cggaaacggt ataactctcc gccagtacgt ttttcagttt catctcttta
    48661 tcgagaccga acagcccctg gtaaaacgat ttcgctacgg cctgagataa cgtgtcattt
    48721 gcgtcatacg gatcgagcgt ggtgaagttc gatcctaccg ccaccaccac atctttggca
    48781 gcgaatgcac aagatgccat caacgctgtc gcaatgccca gcgccactaa cccactacgg
    48841 tgtacagctc ttgccatttt attctccgcc tgaatgttat ctacgcatga atgcgtattc
    48901 tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat tgcaacgaga cggctgccac
    48961 ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc gacagcagta cacgctgcgg
    49021 tcgttgtcgg gacggttcag cgaccggaac tgccgccagt aatttacgcg tataaggatg
    49081 ctgcgggttt tcgaagaccg cgcgccgtgg accaatttca acaatttgtc cgagatacat
    49141 caccgccaca cgatgactaa tccgctctac cacggccata tcgtgggaga taaacagata
    49201 cgcaatgccg aaatcacgct ggagatcgag caacaagttg ataatctgcc cgcgaataga
    49261 aacatccagc gccgagacgg cttcgtcggc aatgatcact tttggattca atgccaacgc
    49321 gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc
    49381 atgttcaggt aacaggccca cgcgctccag caaccacgca acgcgtgcag ccgcttcttt
    49441 acctggcaat aaaccgtgta cgcgcagcgg ttcgataatc gaatcaccga tggtctgacg
    49501 tgggtccagc gaagcgtaag ggtcctgaaa aataaactga atatcccggc gcaatgcctg
    49561 aagtttaccg gatgacaagg tatcgattcg ctgaccgtta aagataattt cgccgccctg
    49621 cgattcgacc aggcgcagca acgcccgccc ggtagtggat ttaccgctgc cagactcgcc
    49681 caccagcgat agcgtttcac caggccagag atcaaaactg actttctcaa cggcatgcac
    49741 ttcccgtgtt acgcgattca acaaaccgct gcgcaaaggg aaacgggtga ccagattacg
    49801 cacccgtaaa acaggttcgc catccaccac cgttttctgc tcgatgggtg gttcctgttt
    49861 cgctggatgt tcaagcgata tcaatgggaa acgtcgggga taatctaacc ctttcatcgc
    49921 accaagttgc ggaacagcag ctaacagcgc acgggtgtaa ggatgttgcg gtgcatgaaa
    49981 aatctgttcg acgctacccg tttccaccgc ctcgccctga tacatcacca gtacccgatc
    50041 ggcaatctct gccaccacgc ccatatcgtg agtgataaag ataacgccca tcgacatctc
    50101 tttttgcaat actttgatta attgcaggat ctgcgcctga atagtgacat ccagcgcggt
    50161 ggttggctca tcggcaatca gcaccgccgg gcggcatgac agcgccatcg caatcatcac
    50221 tcgctggcgc atcccgccag agagttgatg cggataacgt gaaagaatgg tttgtgcctc
    50281 cggaatgcgt acctgatcca gcatccgctt cgcctcgacc atcgcttctt cacgactggc
    50341 gttctgatgc agacgaattg attcggcaat ctgttcaccc acagtaaata ccgggttcag
    50401 cgatgtcatc ggttcctgaa atatcatcgc catatccgca ccgcgcacat ggcgcatttg
    50461 tgcagcgctc tgctcgctaa gttcaatcac ttcgcgactg cgccgccgca acagcatttg
    50521 atcgcactgc actaaaccgc ccgcctgttc caacaagcgc atcaatgcca gcgcagtcac
    50581 tgacttacca gagccggatt cgccaacaat tgccagcgtc tcaccgcgtt gcaggctaaa
    50641 agagagattg cggaccgcag ctattttctg ctggtcctgc ataaaggcaa tattcagatt
    50701 ttcaaccgcc agcacatcac cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt
    50761 tttcacggta gataccggtg gttggcgtat cgcctgcgta gccccaggcg cgatacattc
    50821 cttcggtgtt aaacggtagc gcgacattgc cttcatggtc gatagcgatt aagccaccgc
    50881 taccgccaag cgcagggagt ttttccatta ctacccgctc gcaggcttcc gcgagactta
    50941 atccgccgta atccattaac gcggcgatgt catatgccgc cagcgcgcgg atgaagactt
    51001 cgcccgtgcc ggtacatgaa accgccacac tggcgttatt ggcgtagcat ccggcaccca
    51061 ctaagggact atcgccaact cgtccgggta atttattggt cattccgccc gtggacgtgg
    51121 ctgccgccag attgccgtct aaatccaacg ccacggcccc cacggtgccc attttctgtt
    51181 tttcatccag tggcgcaccg ctatggtcga ggactgttgc cccttcctca cgcgctgcca
    51241 ttagttgttc ataacgcaaa ggcgtggaga aaatctccgg tgagacgcgc tccatgccat
    51301 gagcaaacgc aaaattttct gccccttcgc caatcatcat cacatgcggg ctttgctcca
    51361 tcaccagccg ggcggcaaga accggattac gcagatgact aacgcccgcc accgcaccgg
    51421 ctttcagggt gttaccatcc atcacacagg cgtccagttc atgggtttca tcacgcgtaa
    51481 agacagcgcc aattccggcg ttaaacagtg gacactcttc cagcagacgc accgcttccg
    51541 tcaccacatc cagcgcactt tcgcccgctt ccagcatttt ctgcccggtt tcaacaatgg
    51601 cagacagcgc ctcgatgtag cgtaattcct gttgcagact catctgcgcg cggctaattg
    51661 cacctgcgcc gccatgaatt gcaatgactg ctttgcccat agtattcgtc cattaagggt
    51721 ggctcgccac ttttgctata aatatcagaa tttttatgaa ttactcattc aatttttgaa
    51781 tatagaaaga tggatatggc atgtaaaggc aggacccagc gctacgcccg aggtggcata
    51841 cagtctgtgg cgtttttctg acataatagg caaattcgat tttgcctccg caggagtgtt
    51901 ttcatggaat ttaccaccgg attgatgtcg ctcgacaccg cgcttaatga gatgctttct
    51961 cgcgttaccc cactgaccgc ccaggaaacg ctgccactgg tacagtgttt tggtcgtatt
    52021 ctggcgagcg atgtcgtttc gccactggat gtcccggggt ttgataactc cgctatggac
    52081 ggctacgcgg tgcgtttagc cgatattgcc tccgggcaac cgctgcccgt tgccggtaaa
    52141 tcctttgccg gtcagccgta ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg
    52201 accggtgcgc cggtgccgga aggctgcgaa gcggtggtga tgcaggaaca gaccgaacag
    52261 actgacgctg gcgtgcgttt tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc
    52321 ggtgaagata tctctgcagg tgcggttgtt ttcccggcgg gaacacgcct gactaccgca
    52381 gagctgccag taattgcttc actggggatt gccgaagttc cggtgattcg taaagtgcgt
    52441 gtagcgctgt tttctaccgg tgatgaactc cagttgccag gccagccgct gggcgacggc
    52501 caaatctacg ataccaaccg tctcgccgta cacctgatgt tagaacagtt gggatgcgag
    52561 gtaatcaact tagggattat ccgcgacgat ccccatgccc tgcgcgccgc atttattgaa
    52621 gccgacagcc aggcggatgt ggtgatcagt tccggcggtg tttcagtggg tgaagcggat
    52681 tacaccaaaa cgattcttga agagctgggg gagatcgcct tctggaagct ggcgattaaa
    52741 ccaggtaaac cgttcgcgtt cggtaaactc agcaatagct ggttctgcgg cctgccgggc
    52801 aacccggttt cagcaacgct gaccttctat caactggtac agcctttgct ggcaaaacta
    52861 agcggcaata ccgccagcgg cctgcccgcg cgccagcgtg tacgcacggc atcgcgtctg
    52921 aaaaaaacgc caggacgcct tgatttccag cgcggcgtgc tgcaacgcaa cgccgatggc
    52981 gaactggaag tgacgaccac cggacatcag ggttcacata tatttagctc ctttagcctc
    53041 ggcaactgct ttatcgtgct ggagcgcgaa cgcggcaatg tggaagcggg cgaatgggtg
    53101 gaagtagaac cgtttaacgc gttgttcgga ggcctgtaat ggcggaactc agcgatcagg
    53161 agatgctgcg ctataaccgg caaatcattc tgcgcggctt tgattttgat ggtcaggagg
    53221 cgctaaaaga ttctcgtgtg ctgatcgtcg gcctcggtgg actcggctgc gcagcctcac
    53281 agtatctggc aagcgccggt gtcggcaacc tgacgctgct cgacttcgac acggtttcgc
    53341 tctcaaattt acagcgccaa acgctgcaca gcgatgccac agtcgggcaa ccgaaggtgg
    53401 attctgcccg tgacgccctg acgcggatca acccacatat cgcgattacg ccagtcaatg
    53461 cactgctgga tgacgcagaa cttgctgcga tgattgcaaa acacgatctg gtgctcgact
    53521 gtacggataa cgttgcggta cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc
    53581 cgctggtttc cggcgcggca attcgtatgg aaggtcaaat cacggtcttc acttatcagg
    53641 acggcgaacc gtgctatcgc tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg
    53701 tagaagcagg cgtgatggca ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag
    53761 cgatcaaact gctggcaggt tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg
    53821 cgatgacctg tcagtttcgt gaaatgaaac tgatgcgtaa cccggggtgt gaggtgtgtg
    53881 ggcagtaatt gccacctcag gccagataag acgtagcagg catcggtgca ctccgccacg
    53941 tcatcagcaa tttatgagga tgcagaacgt ccggttaaat cgacgttctg ccaaacgctc
    54001 ccagccagtc ctgttcaaac ttcgccacag cggcatcaac cgccggatag ctaatcatct
    54061 gttgtgccac atccagcggc agagtaattg attcacaccc tgccagtaag cagtccagcg
    54121 cctgacgcgg ggttttgaaa ctcgcggcta acactttcgc ctgcggcgca tgcattttca
    54181 ataactggtg taagtcggtc acagtctgaa taccgctacc gccctgagca tcaatacgat
    54241 taacgtaagg cgcaacatat tccgcacctg ccagcgccga cagcagccct tgtgctgcgc
    54301 catataccgc ggttcccagc gtcggaatcc cttccgcttt taacatctta atagctgcca
    54361 gcccctcagc ggtcaccgga actttcacca cgatatccgc aataatagaa cgcaatttac
    54421 gtgcgtcatt caccatccct tcagcagtgg tagccattac ctgggcaaac agacgcccct
    54481 gaccgcccat cgcttcatga agttccggaa gcacaacctc cagcggtttt ttacccgcgg
    54541 cgataatgct tgggttagtg gtcacacccg ccagcggaaa aatacgtgac agcgccttca
    54601 ccgcaacaac gtctgaagta tccagataca gttccataac catcctcaaa atgtactttt
    54661 tgtctgaatg tagactatca cgggaaaact ctctgaacag ttgacgcaca tcaatctaac
    54721 tttcattcga aagtatttta atgtttgaac gaaacagaga ggcaactatg attttcaata
    54781 ttcagcgcta ctcgacccat gatggccccg gtatccgcac ggtcgtattt cttaaaggct
    54841 gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg caagatctgc
    54901 tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg cgctaaggcc gcgccggaag
    54961 tgattgagcg cgcgctgaat ggtttgctta ttcatcggga aaagttaacc ccggagcatc
    55021 tgacggcgtt aaccgactgc tgtccgacac aggcgttaac cgtgtgtggt gaagtgaaaa
    55081 gcgttgagga gatcatggcg accgttttgc gcgataaacc gttttacgat cgcagcggcg
    55141 gcggcttaac gctttccggc ggtgaaccat tcatgcagcc ggaaatggcg acggcgctgc
    55201 tgcaagccag ccacgaggcc ggcattcata ctgcggtaga aacctgtctg catgtgccgt
    55261 ggaaatatat cgccccttct ctgccctata tcgatctgtt tcttgccgat ttaaaacacg
    55321 ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc cgccagagtg ctggataacc
    55381 tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg attcagggct
    55441 ttaatgccga cgaaacctct gtaaaagcca ttaccgattt tgccgccgac gagctgcacg
    55501 ttagcgaaat tcatttcctg ccctaccaca cgctgggcat caacaaatat tacttactta
    55561 atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc gactttgccc
    55621 agcagtatgc ctgccagaaa gggttaaccg cgaccttacg aggataacaa ccatgaccac
    55681 actgaaactg gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc tggtgcatat
    55741 tgtgaaaccg ccggtctgta ccgagcgcgc gcagcactat actgagatgt atcaacaaca
    55801 tctcgataag ccgatcccgg tacgtcgcgc gctggcgctg gcgcatcacc tggcgaatcg
    55861 caccatctgg atcaagcacg atgagttgat cattggcaac caggcaagcg aagttcgcgc
    55921 cgcgccgatc ttcccggaat ataccgtctc ctggatcgaa aaagagattg atgatctggc
    55981 ggatcgtccg ggtgctggtt ttgcggtgag cgaagagaac aaacgtgttc tgcatgaagt
    56041 gtgcccttgg tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt ttaccgatga
    56101 gcaaaaaggt ctgctggcga ccggaatcat taaagcggaa ggcaatatga cctccggcga
    56161 tgcgcacctg gcggtgaatt tcccgctgct gctggaaaaa gggcttgatg gtctgcgcga
    56221 gaaagtagcg gaacgtcgct cgcgcatcaa cctgacggtg ctggaagatt tacatggcga
    56281 gcaattcctg aaagcgattg atatcgtgct ggtagcggtc agtgaacata ttgaacgttt
    56341 cgctgccctg gcgcgtgaaa tggccgcaac cgaaacccgc gaaagccgtc gcgatgaact
    56401 gctggcgatg gcagaaaact gcgatcttat cgcccaccaa ccgccgcaga ctttctggca
    56461 ggcgctgcaa ctgtgttact tcatccagtt gattttgcag attgaatcta acggtcactc
    56521 agtatctttt ggtcgtatgg accagtatct gtacccgtat tatcgtcgcg acgttgaact
    56581 caaccagacg ctggatcgtg aacacgccat cgagatgctg catagctgct ggctgaaatt
    56641 gctggaagtg aacaagatcc gctccggctc gcactcaaaa gcctctgcgg gaagtccgct
    56701 gtatcagaac gtcactattg gcgggcaaaa tctggttgat ggtcaaccaa tggacgcggt
    56761 gaatccactc tcttacgcga tcctcgaatc ctgcggtcgc ctgcgttcca ctcagcctaa
    56821 cctcagcgtg cgttaccatg caggaatgag caacgatttc ctcgacgcct gcgtacaggt
    56881 gatccgctgc ggcttcggga tgccggcgtt caacaacgac gaaatcgtga tcccggaatt
    56941 tattaaactc ggtattgaac cgcaggacgc ttacgactat gcagcgattg gttgtatcga
    57001 aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc atgagcttta tcaacttcgc
    57061 ccgtgtgatg ctggcggcgc tggaaggcgg tcgtgatgcc actagcggca aagtgttcct
    57121 gccacaagaa aaagcgttgt cggcaggtaa cttcaacaac ttcgatgaag tgatggacgc
    57181 gtgggatacg caaatccgtt actacacccg caaatcaatc gaaattgagt atgtcgtcga
    57241 caccatgctg gaagagaacg tacacgatat tctctgctcg gcgctggtgg atgactgtat
    57301 tgagcgagcg aaaagtatca aacaaggcgg cgcgaaatat gactgggttt ctggcctgca
    57361 ggttggcatt gccaacctcg gcaacagcct ggcggcagtg aagaaactgg tatttgaaca
    57421 gggcgcgatt ggtcagcaac agctggctgc cgcactggcg gatgacttcg acggcctgac
    57481 tcacgagcag ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca acgacgatga
    57541 taccgtcgat acgctgctgg ctcgcgctta tcagacctat atcgacgaac tgaaacagta
    57601 ccataaccca cgctacggtc gtggtccggt tggcggcaac tattacgcgg gtacgtcgtc
    57661 tatctccgct aacgtaccgt ttggcgcgca gactatggca acaccggacg ggcgtaaagc
    57721 ccacaccccg ctggcagaag gcgcaagccc ggcttccggt actgatcatc ttggccctac
    57781 tgcggtcatt ggctcggtgg gtaaactgcc tacggcagcg attctcggcg gcgtgttgct
    57841 caaccagaaa ctgaatccag caacactgga gaacgaatct gacaagcaga aactgatgat
    57901 cctgctgcgc accttcttcg aggtgcataa aggctggcat attcagtaca acatcgtttc
    57961 ccgcgagacg ctgctggaag cgaaaaaaca tccggatcag taccgcgatc tggtagtgcg
    58021 tgtcgcgggt tattccgcct tcttcaccgc gctctctcca gacgctcagg acgatatcat
    58081 cgcccgtact gaacatatgc tgtaatcttc tcagcccggc ggcgtcgtcg ccgggctaaa
    58141 tcattcacac catcaatttt agcctcgctt tcattcgaaa tataatttgt gctctgcgtc
    58201 acattgttat tacactgtta cggtttcatt ttcgagccag gagcacacct atgagcgtaa
    58261 aagttatcgt cacagacatg gacggtactt ttcttaacga cgccaaaacg tacaaccaac
    58321 cacgttttat ggcgcaatat caggaactga aaaagcgcgg cattgagttc gttgtcgcca
    58381 gcggtaatca gtattaccag cttatttcat tctttcctga gctaaaggat gagatctctt
    58441 ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa gcaactgttc cacggcgaac
    58501 tgacccgaca tgaatcgcgg attgttattg gcgagttgct aaaagataag caactcaatt
    58561 ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa gcattcgtcg
    58621 cactgatggc aaaacactac catcgcctga aacctgtaaa agattatcag gagattgacg
    58681 acgtactgtt caagttttcg ctcaacctgc cggatgaaca aatcccgtta gtgatcgaca
    58741 aactgcacgt agcgctcgac ggcattatga agccggttac cagtggtttt ggctttatcg
    58801 acctgattat tcctggcctg cataaagcga atggtatttc gcggttactg aaacgctggg
    58861 atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg gagatgctga
    58921 aaatggcgcg ttattccttt gcgatgggca atgctgcgga aaacattaaa caaatcgccc
    58981 gttacgctac cgatgataat aatcatgaag gcgcgctgaa tgtgattcag gctgtgctgg
    59041 ataacacatc cccttttaac agctgacctc tctaccggag ccatgcgctc cggttttccc
    59101 ttctttttac atcctgtaca tcacatcaaa tcattaaact tgcattaact tatttataac
    59161 ttatattgtc acatgcaaat cattatagtt tcgaatgaaa gttaatgcga ggtggttatg
    59221 gcttctactt ttaccagcga cacattgcct gccgatcaca aagcagctat ccgtcagatg
    59281 aagcacgcgc tgcgggcgca gcttggcgac gtccagcaga tctttaatca gctaagcgat
    59341 gacattgcca cgcgagtggc tgaaatcaac gcactcaaag cacagggcga tgccgtctgg
    59401 ccggtgctgt cttatgccga tatcaaagca ggtcatgtta ctgcagagca gcgcatacag
    59461 attaaacgtc gcggttgtgc ggtgataaaa ggccatttcc cccgcgaaca agcgctaggc
    59521 tgggatcagt cgatgctgga ctatctggac cgcaaccgct ttgacgaggt ctacaaaggc
    59581 cccggcgata atttcttcgg gacgctcagc gcttcacgtc ccgagattta ccccatctac
    59641 tggtcgcagg cgcaaatgca ggctcgccag agtgaagaaa tggcgaatgc gcagtcgttt
    59701 ctcaatcgtc tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc ggatgtgagc
    59761 gtcatctacc ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa aggtcttgga
    59821 gcgcataccg actccggggc actggaacgc tggctgcttc cagcgtatca gcgcgttttc
    59881 gccaacgtct ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc acatcgtacg
    59941 gaagttgaag agtacacggt ggacaacacc accaaatgtt ccgtgtttcg gacattccag
    60001 ggctggacag cgctctctga tatgctgcct ggtcaggggc tgctgcacgt cgtgcccatt
    60061 cctgaagcca tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc ggaggatgaa
    60121 ctgtgcggcg tagcgcccgg aagagtattg ccggtatcag agcaatggca tccactgttg
    60181 attgaggcgt taaccagcat tccaaaactc gaagccggag actccgtctg gtggcactgc
    60241 gacgtcatcc attccgttgc ccccgttgaa aatcaacagg gctggggcaa cgtgatgtac
    60301 attcctgcgg caccgatgtg cgagaaaaat cttgcctacg cgcacaaggt gaaggccgca
    60361 ctggaaaaag gcgcatcgcc gggcgacttc ccgcgcgagg actatgaaac aaactgggaa
    60421 ggacgcttta cgcttgccga cctcaacatt cacggtaagc gagcgttggg aatcgacagc
    60481 tgagtgtcgt acagcccttc ccgttccacg gcggtgttcc cttttcctgg gcgaacccgc
    60541 cgcactgact cccgcaccgc caatcgtccg tgcagcagca gcgagcccac cggagcatcc
    60601 gggcgaatca gccgctgttg caacatttgc accgcttcat tgcctagctc atcgcgtggc
    60661 acatgcacag ccgtcagggg aacatcctcg attgccgcaa ggttaaagcc gtcgatactc
    60721 atcaccgaga catcctgtgg tacgcgcaga ccacgacgat gtagcgcatt taccgttccc
    60781 gccgccataa aatccccgcc gaccagaaac gccgtcggca acgtcttgcc attggcggca
    60841 tccagccacg ctccgacctt ttcttccgct tctttagcgc taaaactgct gatattcagc
    60901 aaatgacggt catggtgaaa ggccagatta tgctgacgcc atgattcttt aatccctgca
    60961 aaccgctgct ccatagtgta gcggcgcagg cacatcacat tcatcacctc ccggtgaccc
    61021 atttcaaaca gatagcgggc ggcgaacgcg ccaattaacc gatggtccgg cgcaatcgcc
    61081 gggaggcgca ttcgttcatc acggcagttg atcagcacac aaggcttagc gagatcggcg
    61141 gccagatcat ggatgtgcgg atcgtcaatc cccagcagga tcgccgcctg cgtatctgct
    61201 tcattcatcc gcgagagaaa tagcgcggga tcgctgtcaa actcctccag cgcgcagtaa
    61261 cgcagccgca cttcatgcgg cgataacgct ttgctgatgc tctggatcac acggtaataa
    61321 aagatatcag atcgttggtc aaaagcgcgt tgcggcgcga aaataatcag gttgttcagc
    61381 aacatacgac ctgctgccat gccatccatc acgcccaact cacgcgcgca ggccagcacc
    61441 tgttcgcgag cgcgtgcact ggtattcgct ttgccagcca gcacccgcga aaccgtactc
    61501 ggcgatagcc cggtacgtgc ggcgatatcg gcgattttca gcttcttgtc cattttgtga
    61561 tctgcctcta attcggaaat gacagaattt tcatgagcaa aaaagcccat tctgtctggc
    61621 attaaatcca tttcagtaat gtatatcagt cactcatgga aaaacgtgca caaaacccgc
    61681 tttctttcgc ttttagggtc tcttactgtg aattggtaga acaacttcca tacaaaaata
    61741 atggaaaaac gaacaataaa gactttattt atcaaaaagt taaaagtggt gtgacaactc
    61801 cattctgacg ttgccacaca aaccctatac cattctgtgg agtgcatatg agtcagggta
    61861 tcaataacga tatgactgcc agtaaaccgc gccatatcgt caaaaatctg cgttggtgga
    61921 tactggtcct ttttttgctc ggtgttactg tcaactacat cacgcgtaac tcgctgggta
    61981 ttctcgcccc cgaactgaaa agcaacctgg ggattaccac cgaacaatat tcctggattg
    62041 taggtgcttt ccagctcgcc tataccctat tccaaccgtt gtgtggatgg ctgattgacg
    62101 tcattggcct caaactgggc tttatgatct gcgcgggact gtgggcgctg gcctgtctga
    62161 tgcatgcggg ggcgggaaac tgggtacatc tggcgctgtt gcgcttcttt atgggcggtg
    62221 cggaagccgc ggcaacccca gcaaacgcca aaaccatcgg tgaatggttt ccaaaatcag
    62281 aacgcccgat cgccgcaggc tgggcaggcg tcggtttttc catcggtgcc atgctggccc
    62341 caccgattat ctattttgcc cacgcttcat tcggctggca gggtgcattt atgttcactg
    62401 gcatcctggc gatgctgtgg gtggtgctgt ggtgggcgtt ctatcacaac ccggataagc
    62461 atccgaacct cagcaaagcc gagctggact ttatccgcca ggataacgaa gcgcccccga
    62521 ttaaattacc ctttctgacc gcactgaaaa ccgtgggcaa aaataaacgc ttttacggta
    62581 tcgctatccc ggcctttatg gcagaaccgg cgtgggcggt gatgagtttc tgggtgccgc
    62641 tatacctgtc caaaacctat ggcatggacc tgaagcagat cgcattattc gcctggttgc
    62701 cgttcctcgc cgctgatctt ggcagtattg ccagcggcta tctgaccaaa ctttacaccc
    62761 gcctgtttgg gctgagtcgt gtgaactccg ttgtcgcatc atcggtcagt ggcgcattct
    62821 tgatggtttc gctggcggtg atggccttta ccaataaccc gtatatcgcc atcgtcctta
    62881 tttccatcgg cggcttcggt caccagatca tctcctgcat gctgagcgca ctggtggtgg
    62941 aatccttcga caaaggtcag atggccactg ttaacggcat gcgcggttct gccgcgtgga
    63001 tcgcaagttt cctgttctcc ctgattatcg gggttaccgc tgacaaaatc ggctttaacc
    63061 ccctgtttat cgccatgggc ttctttgacc tgattggcgc tttcttcctg gtcaccttta
    63121 ttgctgaacg tcgcgcaaaa cgcgcctgag agtggatgta aacatgaaaa cgctgaaaaa
    63181 ctggaccctg cacaaacagt ctgcccacca cgttgaactg ctggtcgatg gccagcatat
    63241 cctgtgcctg tacgtgctgg aagaaaatct gttccgcgtg ctgttaaaac gcaaaggcga
    63301 gctggcgctg gatcgcacct ggagcattgc tccggcgcag gatgtgccat gggaagggcg
    63361 cagccgcgaa gatctcagcg gctttagcct gcccgcgtgg acattgatcc agcacgacga
    63421 caccgtggtt atcgccaccg agtcactgcg tgccaccgtt caccagccgc tgtggctgga
    63481 gtggcactat cgtaacaacg aaggcgaatg gctgccgttg gtgaatgacc gcccgaccag
    63541 cgcttatctg ctgaatgccc acggcgatgg cgtggcacac tatcagagcc gtcgtaaaga
    63601 tgaacgcttt tatggcctcg gagataaagc cggcgacctg caacgcaacg gtaaacgtta
    63661 cgagatgcgt aacctcgacg cgatgggcta caacgccgtt agtactgacc cgctgtacaa
    63721 acacattcca ttcaccatca cccgccgcga tgacattagc ttcggcctgt tttatgacaa
    63781 cctcagcagc tgctggctgg atctgggcaa cgagatcgac aactaccaca ccgcctatcg
    63841 ccgctggcag gcggaagcgg gtgatatcga ctactacctg tttaccggcg aacgcgtact
    63901 tgatatcacc aaagccttcg tacgcctgac cggcaaaacc ctgttcggac cgaaatggag
    63961 cctgggctac agcggttcaa ctatgcacta caccgatgcg ccggacgcac aaaatcagtt
    64021 gatgaacttt atccgcctgt gtgaagaaca tgcgattccc tgcgattcgt tccagctctc
    64081 ttccggttat acctcaatta acggcaagcg ctatgtgttc aactggaatt acgacaaagt
    64141 gccgcagccg aaagtgatga gccaggcatt tcacaatgct ggtttgcacc tggccgccaa
    64201 tatcaagccg tgcctgttgc aggatcaccc acgctataac gaagtggcag aaagaggact
    64261 gttcatccgt gattcagaaa cagatgaacc agaacgttcc agcttctggg atgacgaagg
    64321 ttctaacctc gactttacta atccgcagac catccagtgg tggcaggaag gcgtgaccac
    64381 acagcttctg gagatgggta tcgactccac ctggaacgac aacaacgaat tcgaagtatg
    64441 ggacggtgaa gcacgctgtc acggctttgg tcagacgatc gcgatcaaac atattcgccc
    64501 ggtgatgcca ctgctgatga tgcgagcctc gatggaagcc cagcaacgtt tcgctccgga
    64561 aaaacgtccg tacttgatct cccgctccgg ctgcgcggga atgcagcgct atgtgcagac
    64621 ctggagcggc gataaccgca ctagctggga cactctgcgc tacaacaccc gcatgggtct
    64681 ggggatgagc ctctccgggc tgtataacct cggtcacgac gtcggcggtt tctccggtga
    64741 taagccggac ccggaactgt ttgtacgctg ggtacaaaac ggcgtgatgc atccgcgctt
    64801 tacgattcac tcgtggaacg atgaccatac cgtaaacgaa ccgtggatgt atccgggcgt
    64861 cactccggcg atccgcagcg ctattgagtt gcgctatcgc ctgctgccct acttttatac
    64921 cctgatgtgg caggcatacg ccgatgatga accaatgctg cgtccgactt tccttgacca
    64981 cgagcacgac gttcagactt ttgaagagtg tgatgacttc ctgcttggtc gcgatatttt
    65041 ggttgccagc gtcgtagagc aaggcgagcg ccagcgccgt gtatggctgc cagataacga
    65101 aactggctgg tacgatttct acaacggcga atggttcagt ggcggtcagt ggatcaccct
    65161 cgacgcaccg ctggagaaac tgccgctgat ggtgcgtgcc ggggctggtc taccgctcag
    65221 caaacgcatc acctatgtga gcgcagaaca agacgatacc cgcgaactga aactgttccc
    65281 gctgaaaggt gtgggcacaa cgtccggcct gctgtttgaa gacgatggcg aaagctgggg
    65341 ctaccaaact ggtaatgcgc tttgggtgga atgggaaatg gtttgcgatg gggcgaccat
    65401 caacctgaaa gtcaacgcgc gcggggatta tcgacctgca tggagtgcac tgaaagtgtc
    65461 attaccagtg gaagaaaaac gcacgctgct ggtcaatggt gttgaaggga gtgaatggat
    65521 gcgttaaatc attaccgggg ccgggaggcg actgcgcctt acccggtcca gccatcgaat
    65581 cctttacctc agtatttcac gctgcaggaa ggcagcaaac ctgtaaaccc cgggagctta
    65641 aataagtaag tgaccggggt gaacaggtga agccaatgca tctgccgcat gaaagacggc
    65701 gggaaattaa ccgtcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc
    65761 gatcacgcgt tccggggtga tttccagaat tcgggtcgcc agggagctta caaactcacg
    65821 gtcgtgagac acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagcgactc
    65881 aatagattcc atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg
    65941 catcattaac ttaccaaaca gcatccgccc tttttctcca ccggaaagca ctttcgctgg
    66001 ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc
    66061 atcatcgcct tcctgcttcc actggctcat ccattcgaac acggtcagat cattttcaaa
    66121 ctcatattcg tggtcctggg cgtagtaacc aatgcgtgcg ttttcagacc attttacggt
    66181 gccgctgtcc ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc
    66241 gttggtaccc agtacagcca gtttttcacc cacttccagc agcaggttga gatttttaaa
    66301 cagtggaccg ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag
    66361 tttcttatcc tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc
    66421 cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt
    66481 ggcgctaaag cggctaacga aagattgcag ctcagcaatc tgcgctttct tcttggcgtt
    66541 atcagccagc agacgctcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc
    66601 tggataaacg cgcagctcgc cgtaatccag atccgccatg tgggtacaaa ccatgttaag
    66661 gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc
    66721 cagccaacga atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatgtc
    66781 tggatcagca aacagcgcct gcgccagcag cacacgcagc ttccagccag gagcaacttc
    66841 actcatcggg ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc
    66901 gcgagcttcc gcagagtaac cgtccatttc gccgtatttc acttccagat cggccacttt
    66961 atagccgtct tcttcactca tttccggcaa agcatagatg cggtcacgct cctgtttcac
    67021 ttcccacaac tctttatgcc ccatgatcac cgtatccaac acagtgaact cttcaaaggc
    67081 aaactgatcc tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa
    67141 cgtcggctct aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc
    67201 gccaatcagg ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg
    67261 cttactgccg aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga
    67321 gatatgacaa accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag
    67381 gtctacactc aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag
    67441 caggaatgaa cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca
    67501 ggcacaaaat ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg
    67561 ctgttgtcgg cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga
    67621 gtcgcctggt tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg
    67681 agtattttgc cgccgagtac cagatggggc tttccaatat gatggaagcg aacccgggtg
    67741 tggatacctt cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc
    67801 cggataccgt tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc
    67861 cgaaaggaac caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagaca
    67921 cgcctatcaa ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccaa
    67981 ccgctaaaat gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg
    68041 caggtccgga taacccgatg ggtctgtatg cactctacat cggtcgcctg tacgctatcc
    68101 atggcaccaa cgccaacttc ggtatcggcc tgcgcgtaag tcatggttgt gtgcgcctgc
    68161 gtaacgaaga catcaaattc ctgttcgaga aagtgccggt cggtactcgc gtacagttta
    68221 ttgatgagcc ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata
    68281 atccgctgtc taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga
    68341 cgaagagtgt gcagacagtg accggtcagc ctgatgttga ccaggttgtt ctggatgaag
    68401 cgatcaaaaa tcgttccggg atgccggttc gtctgaatta atcgtcaaaa cgtaaaacag
    68461 aggcggactc atcatccgcc ttttttattt gccagatcct accaggccgg gagcataacg
    68521 aataaaacgt atcccaccag cgccgcccac aacagcatcg gtattgaata gagatggaaa
    68581 cgccaccaga tacggcgatc gttcgccatc cgtaacgcaa tcaaatttgc cagcgatccg
    68641 ggtaataaac caaagccccc cacgtttacc gcccatgcca gtaataaaga cggcggtaca
    68701 taattgagca gcaaaatggt actcggcacg ttactgatca cctgcgataa accgattgcc
    68761 gttaaccata acccgggttc agataaatga ctcacgttac ccaacacacc ttgcaacgct
    68821 ggaagctggg tcagtaaatg gacgtcgata aacatcgcca taaacaccag cagcagcgtc
    68881 cagtccacac tgagcaccac gcgacgtgcc agcagcgcaa agcccgccgc cacaatcacc
    68941 agtccccaca gctcttgttt taactccagc gccgtcagaa agacgatata cagccccaga
    69001 caactccaca ccagccgcgg tttccactcc ggagtttgca cccccgtatg gtattgcagt
    69061 gcctttccag ggaaacaaca ccagcacagg agcaggagcg tcagcatcat tgcgccagcc
    69121 agtggtgcca tttgggcaat aaatccggca aacgaaagac cagaacgtcc ccagataaga
    69181 atattttgcg ggttgccaat tggcgtcagt agcgaaccag cgttgactgc cagcgcttca
    69241 aaaataatca gccgattaac cgggatctca cacagtcttt ttagcgtgat agtcagcgga
    69301 acaacaataa acagcgcgac atcgttggtc agaaaggtag aaagcagcgc cgccgccagc
    69361 accataaaca tcgccagccg acgctccgta gcaaagcggc gcaccatttt gcgccccagc
    69421 acatcaaaat aaccgcttaa ctccacacct ttggtcagca gcatcaggcc gcttaaggtg
    69481 atgatggtgt gccagtcgat agcagcaggc caggatttcg gtgcaaaagg cacgaaaaag
    69541 cttaatccga taccaacaag aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt
    69601 aaaaaaggca ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg
    69661 tctcttcact aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt tccggactga
    69721 cgcccaacac caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca
    69781 gcttctctcc ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca
    69841 tggttgccag tcgcttaagc attttagcca ccgtcggttg cgaaactccc aaacgagcag
    69901 ccatgtctac ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt
    69961 aatcatcaat aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct
    70021 cttccacgtt cactaactgc gtcacttttt ttgctgttgg cgtacctgcg cgacgactca
    70081 ttgtgcttcc tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa
    70141 accagagttg cgaaggtaca aaaaattaac gttttagcaa tagctatata atatagcctg
    70201 tgctatatct gtatgtaatg caatcatccc tcaaggatcg acgggattag caagtcagga
    70261 ggtcttatga atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta
    70321 cggttaatgc tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaagataaa
    70381 ccttccgata aatagcggcg tcacggtacg ccgcttcact cctgctttta tgcaggcaca
    70441 acgcgttttg gtctgaaaaa ccccactttt tgtcggattt gcaatcccct tcgcaaaaga
    70501 tttgttcgtc agtagttgac ctgaacggcg gcgcgctcta tcttcttgca gccctgcgta
    70561 tattgcggct cgcggatgcg gacccctttc ccctctccac gcactcttgc aggtattgac
    70621 ccttgacgcc agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct
    70681 caaagaatcg cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt
    70741 acagtcgcta ttaattaacc tcggcttagg ttatcccttc agtttgctct acaccgctgc
    70801 gtttacggct atgttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt
    70861 cggtgtcagt tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc
    70921 taatttcaat acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct
    70981 gacgattttt ccgtggtaca gctacctggt cggcttattt atttttgcgc tcggcttaat
    71041 agcaatcagg cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg acagcctgtg
    71101 tctggtattc agtgtggcga cattttttgt tgctcccatg caaaacctgg cctggggcgg
    71161 cgtatttaaa ctgaaagata ccggctatcc ggtatttcgt tttgctaagg atgtcatcgg
    71221 caataataac gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga
    71281 tacctggacg gtcactgccg ttaagccgaa atatcagacc tatgtggtgg tgatcggtga
    71341 aagcgcgcgt cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc
    71401 cagcagtgtt aacggtttga tatttgctga ctacattgcc gccagtggct ccacgcagaa
    71461 atcgcttggc ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc aggataactt
    71521 tgtcaccctg gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggccaaat
    71581 cggcgaatac gataccgcca tcgccagcat cgccaaacgt gcagatgaag tgtacttcct
    71641 gaaagaaggt aattttgaag cagataaaaa cacgaaagac gaagcgttac tggatatgac
    71701 cgctcaagtg ctggcacaag agcactcgca accgcagctg attgttttac atctgatggg
    71761 ctcgcatccg caggcctgcg acaggacaca aggcaaatac gaaacctttg tgcaatcgaa
    71821 agaaacgtcg tgctatctct ataccatgac gcaaacagac gatttactgc gtaagctgta
    71881 cgatcagtta cgcaacagcg gcagcagctt ctcgctggtt tacttttctg accacggtct
    71941 ggcctttaaa gagcgcggca aagacgtgca ataccttgcc catgatgata aatatcagca
    72001 aaatttccag gtgcctttta tggtcatttc gagcgacgat aaagcgcatc gggtgattaa
    72061 agcccgccgc tctgccaatg acttcttagg ctttttctcc cagtggacgg gaattaaagc
    72121 gaaggaaatt aacatcaaat acccgtttat atctgagaag aaagccgggc cgatatacat
    72181 caccaacttc cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc
    72241 gaaaccttaa aaacaaaaat ccgccccgag aggcggattt tttatatcac cgaagtgatt
    72301 agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga atacggctct
    72361 gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga ccagcaccgt
    72421 aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg gtctggaatt
    72481 taccataacc cacacccact acaccgtaga tgcttgccca gtcgttaatg cggtaagccg
    72541 gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt gcagtacggc
    72601 ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct tcttcatagc
    72661 ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg ctctgtgcgt
    72721 aaccgccagt tacggtagaa gttgcagcta cggaagtacc tgcggtgaaa gccagaactg
    72781 cggccagtgc tgaaagacat gcaatttttt tcataaccac ctcaaatgtg attcaaataa
    72841 gtcctaagtt ttaaatatat caaaaattaa tgggaaactc ttcgcgattt gtgatgtcta
    72901 acgggccatt tcatgtaaca gaacgtttcc atacaccgct atccatctaa atttaaatca
    72961 ctttttcaga gaactgcgta agtattacgc atgttttccc tgtcattcat ccagattatt
    73021 cctaatcacc agactaatga ttccatcaat cctggcgcat tttagtcaaa acgggggaaa
    73081 attttttcaa caaatgctca accagcattg ggtatatcca gtacactcca cgctttactt
    73141 aagtctagat atttgtggga gaaaggatgc ctggttcatt acgtaaaatg ccggtctggt
    73201 taccaatagt catattgctc gttgccatgg cgtctattca gggtggagcc tcgttagcta
    73261 agtcactttt tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg gcattaggaa
    73321 cgctgatcct catcgcgttc tttaagccat ggcgactgcg ctttgccaaa gagcaacggt
    73381 taccgctgtt gttttacggc gtttcgctgg gggggatgaa ttatcttttt tatctttcta
    73441 ttcagacggt accgctgggt attgcggtgg cgctggagtt caccggacca ctggcggtgg
    73501 cgctgttctc ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg gttcttggtc
    73561 tgtggttcct gctaccgctg gggcaagacg tttcccatgt cgatttaatc ggctgtgcgc
    73621 tggcactggg ggccggggct tgttgggcta tttacatttt aagtgggcaa cgcgcaggag
    73681 cggaacatgg ccctgcgacg gtggctattg gttcgttgat tgcagcgtta attttcgtgc
    73741 caattggagc gcttcaggct ggtgaagcac tctggcactg gtcggttatt ccattgggtc
    73801 tggcggtcgc tattctctcg accgctctgc cttattcgct ggaaatgatt gccctcaccc
    73861 gtttgccaac acggacattt ggtacgctga tgagcatgga accggcgctg gctgccgttt
    73921 ccgggatgat tttcctcgga gaaacactga cacccataca gctgctggcg ctcggcgcta
    73981 tcatcgccgc ttcaatgggg tctacgctga cagtacgcaa agagagcaaa ataaaagaat
    74041 tagacattaa ttaaatttac atttctgcat ggttatgcat aaccatgcag aatttctcgc
    74101 tacttttcct ctacaccgtc tttatatatc gaattatgca aaagcatatt tattccgaaa
    74161 attcctggcg agccaataaa taagaattgt tcttatcaat ataactaacc cattgaattt
    74221 ttattagttt tgtttttcac gcttgttacc actattagtg tgataggaac agccagaata
    74281 gcggaacaca tagccggtgc tatacttagt ctcgttaatt actgggacat aacatcaaga
    74341 ggatatgaaa ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa tctgctttat
    74401 acccgcaacg atgtctccga cagcgagaaa aaagcaacag tagagttgct gaatcgccag
    74461 gttatccagt ttattgatct ttctttgatt accaaacaag cgcactggaa catgcgcggc
    74521 gctaacttca ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact gatcgatcat
    74581 ctggatacca tggcagaacg tgcagtgcag ctgggcggtg tagctctggg gaccactcaa
    74641 gttatcaaca gcaaaactcc gctgaaaagt tacccgctgg acatccacaa cgttcaggat
    74701 cacctgaaag agctggctga ccgttacgca atcgtcgcta atgacgtacg caaagcgatt
    74761 ggcgaagcga aagatgacga caccgcagat atcctgaccg ccgcgtctcg cgacctggat
    74821 aaattcctgt ggtttatcga gtctaacatc gaataaatcc atcgttgatg gtgcagaact
    74881 ttagtacacg ataaaagcgg cttcctgaca ggaggccgtt ttgttttgta gcccgcctca
    74941 acgcacttat ttagtgcatc catctgctat ccccagctgg ttaagtaaat tttttgtatc
    75001 cacatcatca cacaatcgtt acataaagat tgttttttca tcaggtttta cgctaaataa
    75061 tcactgtgtt gagtgcacaa ttttagcgca ccagtttggt gccccagaat ggtgcatctt
    75121 cagggtattg ccctataaat cgtgcatcac gtttttgccg catctcgaaa aatcaatgag
    75181 ttgcaaaact ggcacgattt tttcatatat gtgaatgtca cgcaggggat cgtcccgtgg
    75241 atagaaaaaa ggaaatgcta tgaagtctgt attaaaagtt tcactggctg cactgaccct
    75301 ggcttttgcg gtttcttctc atgccgcgga taaaaaatta gttgtcgcga cggataccgc
    75361 cttcgttccg tttgaattta aacagggcga taaatacgtg ggctttgacg ttgatctgtg
    75421 ggctgccatc gctaaagaac tgaagctgga ttacgaactg aagccgatgg atttcagtgg
    75481 gatcattccg gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca tcaccattac
    75541 cgacgagcgt aaaaaagcga tcgatttctc tgacggctac tacaaaagcg gcctgttagt
    75601 gatggtgaaa gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg ggaaagtggt
    75661 tgctgtgaag agcggcactg gctccgttga ttacgcgaaa gcaaacatca aaactaaaga
    75721 tctgcgtcag ttcccgaaca ttgataacgc ctatatggaa ctgggcacca accgcgcaga
    75781 tgccgttctg cacgatacgc caaatatttt gtacttcatc aaaaccgccg gtaacggtca
    75841 gttcaaagcg gtaggtgact ctctggaagc acagcaatac ggtattgcgt tcccgaaagg
    75901 tagcgacgag ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg agaacggaac
    75961 ttacaacgaa atctacaaaa aatggttcgg tactgaaccg aaataataac gctacgcctg
    76021 taaagcgcac tggcagttcc ctctcccctc tggggagagg attagggtga ggggcgcaaa
    76081 cccgctccgg ggccattaat taccctgaat ttgattattt acaccacggt aacaggaaca
    76141 acatatgcag tttgactgga gtgccatctg gcctgccatt ccgcttctga ttgaaggtgc
    76201 caaaatgacc ctgtggattt cggtcctcgg tttggctggc ggtctggtga tcggattgct
    76261 ggcaggtttt gcacgcacct tcggtggttg gatagccaac cacgtcgcgc tggtctttat
    76321 tgaagtgatc cgcggcacac ctatcgtcgt ccaggtgatg tttatttatt tcgccctgcc
    76381 gatggcgttt aacgacttac gcatcgaccc gtttactgcg gcggtggtca ccatcatgat
    76441 caactccggc gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta ttcacaaagg
    76501 ttttcgtgaa gcaggactgg cgctcggtct ttcacgttgg gaaaccattc gctacgtcat
    76561 tttaccgctg gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga tcatcagtat
    76621 aaaagatacc tcgctgttta ttgttatcgg cgtggcggaa ctgacccgtc aggggcaaga
    76681 aattattgcc ggtaacttcc gcgcccttga gatctggagc gccgtggcgg tgttctatct
    76741 gattattacc ctggtgctga gctttattct gcgtcgtctg gaaagaagga tgaaaatcct
    76801 gtgattgaat ttaaaaacgt ctccaagcat tttggtccaa cccaggtgct gcacaatatc
    76861 gatttgaaca ttgctcaggg cgaagtcgtg gtgattatcg ggccgtccgg ttccggtaaa
    76921 tcgaccctgc tgcgctgcat caacaaactg gaagaaatca cttccggcga tctgattgtc
    76981 gatggcctga aggttaacga tccgaaagtt gacgagcgcc tgattcgcca ggaagcaggt
    77041 atggtgttcc agcagtttta cctcttcccg catctgacgg cgctggaaaa cgtcatgttt
    77101 ggtccgctac gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc gcgtgagctg
    77161 ctggcgaaag tcggcctggc agaacgcgct caccactacc cttccgagct ttctggtggt
    77221 cagcaacagc gtgtggcgat tgcccgtgcg ctggcggtaa aaccgaaaat gatgctgttc
    77281 gatgaaccga cttccgctct tgacccggaa ctgcgccatg aagtgctgaa ggttatgcag
    77341 gacctggctg aagaagggat gacgatggtg atcgtgaccc acgaaatcgg ttttgccgag
    77401 aaagtagctt cgcgcctgat ctttatcgac aaaggccgga ttgcggaaga tggcgatccg
    77461 caggtgttaa tcaagaaccc accgagccag cgcttgcagg aatttttgca gcacgtctct
    77521 taacttctca gcctcccttt cccccgaaag ggaggcacac cagattcctc tcatttaaaa
    77581 tcgcccctcc cccagcatct atacttatct ttttgctctg ttttctcact ggaggagtca
    77641 tgcggtggat cctgttcatc ctcttctgcc tgctgggcgc acctgcccac gcggtatcca
    77701 tacccggcgt tacaaccaca acgacaacgg actcaacgac tgaaccggcc ccggaaccgg
    77761 atatcgaaca aaaaaaagcg gcctatggcg cactggcgga tgtgctggat aatgacacct
    77821 cgcgtaaaga gttgatcgac cagttgcgca ccgtcgccgc gacgccccct gcggaacccg
    77881 taccgaagat cgtgccgccg acgctggttg aagagcaaac cgtgctgcaa aaagtcaccg
    77941 aagtcagccg ccattatggt gaagcccttt ccgcccgctt cggacaactt tatcgcaata
    78001 tcaccggctc cccgcataag ccgtttaatc cacaaacctt cagcaatgcg ttgacccatt
    78061 tttcaatgtt agcggtatta gtgtttggtt tttactggct gattcgtctg tgcgcactgc
    78121 cgctgtatcg caaaatgggc cagtgggcgc ggcaaaaaaa tcgcgaacgc agcaactggt
    78181 tgcagcttcc ggcgatgatt atcggggcgt ttattatcga cctgctgtta ctggcactga
    78241 cattatttgt cggtcaggtt ttaagcgaca acctgaatgc gggcagtcgc accatcgctt
    78301 tccaacaaag tttgtttctc agcgcctttg ccctgattga atttttcaaa gccgtactac
    78361 gcctgatttt ttgcccaaac gtggcggaac tgcgcccgtt cacgatacag gacgagagcg
    78421 cccgttactg gagtcgccgc ctgagctggt taagcagttt gataggttat ggcctgattg
    78481 tggccgtgcc gattatctct aatcaggtga atgtacaaat tggcgcgctg gcgaacgtca
    78541 tcattatgct gtgcatgacc atctgggcgt tgtacctgat ctttcgtaat aaaaaagaga
    78601 ttacccaaca tttgctcaac tttgcggagc attcgctggc ctttttcagc ttgtttatcc
    78661 gcgcctttgc gctggtgtgg cactggctgg caagcgccta ttttatcgtg ctgtttttct
    78721 tttcgttgtt cgatccgggc aacagcctga aatttatgat gggtgcaacg gtgcgcagcc
    78781 tggcgattat tggtatcgca gcatttgttt ccggtatgtt ttcccgctgg ctggcaaaaa
    78841 ccatcaccct ctcgccacat actcagcgta actatccaga actgcaaaaa cggttgaatg
    78901 gctggctgtc ggcggcgctg aaaacggcgc gtattctgac agtctgcgtg gcggtaatgt
    78961 tgctgttgag cgcatgggga ttgttcgatt tctggaactg gctgcaaaac ggcgcggggc
    79021 agaaaactgt agatatcctg atccgtatcg cactcattct tttcttctcg gcggttggct
    79081 ggacagtgct cgccagtttg atcgaaaacc ggctggcttc ggatattcat ggccgcccgc
    79141 tacccagcgc ccgtacgcgt accctgctga cgctgtttcg taacgcgctg gcggtgatta
    79201 tcagtaccat caccatcatg attgtgttgt cggaaatcgg cgtcaatatc gcgccattgc
    79261 tggcaggtgc cggtgcattg gggctggcta tctcttttgg ttcgcaaacg ctggtgaaag
    79321 atattatcac cggggtattt attcagtttg aaaacggcat gaacactgga gatttggtga
    79381 ctatcgggcc gttgaccggc actgtggaac ggatgtcgat tcgctccgtg ggcgtgcgtc
    79441 aggataccgg ggcgtatcac atcattccgt ggtcttcgat taccaccttt gctaactttg
    79501 tccgcggcat tggttcggta gtggcaaatt acgatgttga tcgccatgaa gatgctgata
    79561 aagccaatca ggcactgaaa gatgcggtaa cggaattgat ggaaaacgaa gatattcgcg
    79621 ggctgattat tggtgaaccg aactttgccg ggattgtcgg cttaagcaat accgcgttta
    79681 cactgcgtgt ttcgttcacc acgctgccac tcaaacagtg gacggtacgc tttgccctcg
    79741 acagccaggt gaaaaaacat ttcgacctgg cgggcgttcg cgcgccagtg cagacttatc
    79801 aggtgctgcc tgctccgggc gcgaccccgg ctgcaccgct gccgccgggg gagccaacgc
    79861 tttaacgttg gcgattaaca aaacggcggc gctgctcgtc gttcataaag gtccaggcaa
    79921 taaagcgact ctgcttttgc ccctgggcca tctctttttt aaccaccttc accgcgccca
    79981 cgtccgtcag agcacgatac aacggcggta agttttcacc acgagatacc agcgaggtaa
    80041 accacatcac ctgcttcgcg aagcctttgc tctcttcaat catctttttg ataaaggcga
    80101 cttcaccgcc ttcacaccac aactcctgtt gctgaccacc aaagttcagt gcatcgtctt
    80161 tgttcagccc caggttacgg cgtttacgct cactacctgc ccgcgccgca gcggcggaat
    80221 cgtggaatgg cgggttacac aaggtcgcgt cgtattgctc gtttttatgg ataatgccgt
    80281 taaaaatcgc cccactctct ttttgccgac gcagacgaat ggcgcggtta agccccggat
    80341 tagcactgat gatcgcctgc acactgctta acgcctggct gctggtttca ctaccggtaa
    80401 aacgccagcc atattcatgt acgccaatca gcggataaat acagttcgca ccaacgccga
    80461 tatccagaat gctggcattc gctggaattg ttccgcttgc ttcggccagt aaatcggcaa
    80521 ggtgatgaat ataatccgcc cggcccggta ctggtgggca gagaaaaccg tccgggatat
    80581 cccagttcgc tacggcgtaa aaatgggcca gcaacgcctt attgagcgcc ttcaccgcca
    80641 gcggattggc aaagtctacg ctttgctccc cggcgggtgt aagcgtgagg aattgcctga
    80701 gttcaggatt gacctgacaa agcgtggcga gatcgtagcg gctgtgatga cggttgcgcg
    80761 gatgcaaccc cggtttctgg gcggacatag cattctcctt taagcagcgg cgtaagatac
    80821 ccgcataacg cgacagggta aatatctggc tgcgtttatc atttctaaaa accttcacat
    80881 ggctatggtt tatggcaatt caccataaaa atcatcatca tgcatgttat tgatttgtct
    80941 tattttgtaa aaatatatcg ttcaaaaaat aagcgcatct ctgtcaattt tttgcgtcat
    81001 tttcatcgcg caggcgacct acacttatct ggcactaccc gttgtattta tgaggatgtc
    81061 attatgaaaa agtgcctcac actactgatt gccaccgtcc tgagcggaat ctctctcacg
    81121 gcttatgccg cgcaaccgat gagtaacctg gacagcggtc agctacggcc cgccggtacc
    81181 gtttcggcaa ctggcgcatc aaacctaagc gatctggagg ataaactggc agaaaaagcg
    81241 cgcgagcaag gcgcgaaagg ttatgtcatt aattccgcag gcggaaatga ccagatgttc
    81301 ggtactgcaa ccatctacaa ataaccccac caacgtaact gcattcgcct ctggatgcag
    81361 ttttcgcctg ttttgtaatc aaaaaacatc aaatatgaac gcaatgtaaa taaatgtatt
    81421 tctttttcgc gcaatgggtg atagaaaatc gcgccaagtg ataatgctta tcaaaattat
    81481 tatcacttgc acgaacacta tcacgagatt aacagtggca tcgcatccgc agagaggctt
    81541 tctcgtggca gtgaaaattt caacatataa gaaaaagtca cctgcaaaat ggaaaacaat
    81601 cgcaatttcc ctgccagaca atttcattcg ctcacgttct ttgccggtct ttgtattggt
    81661 attacgcctg tggcacaggc actcgccgcc gaagggcaag ctaacgcgga tgacacgctg
    81721 gttgtcgaag catcaacgcc ttcgctttat gcgccacaac aatctgcaga tccgaaattc
    81781 tcgcgtccgg tagcgaatac tacccgcacg atgacggtga tttctgaaca agtgattaaa
    81841 gatcagggcg caaccaacct taccgacgca ctcaaaaacg tccccggcgt gggtgcgttt
    81901 tttgcgggtg agaacggtaa ctccaccact ggcgacgcca tttatatgcg cggtgctgat
    81961 acctctaaca gtatttatat tgatggcatt cgcgatatcg gcagcgtctc gcgcgacacc
    82021 ttcaataccg agcaggtcga agtgattaaa gggccgtccg gcaccgacta cgggcgcagc
    82081 gcaccgacag gctcgatcaa tatgatcagc aagcagccgc gcaatgattc cggcattgac
    82141 gcctccgcca gtattggtag cgcctggttc cgccgcggca cgctggacgt caatcaggtc
    82201 attggtgata ccaccgcggc gcgcctgaat gtgatgggcg aaaaaacgca cgatgccgga
    82261 cgcgacaaag tcaaaaatga gcgttacggc atcgcccctt ctgtcgcttt tggccttggt
    82321 acagcgaatc gtttgtatct taattatctg catgtcaccc agcacaacac gccagacggc
    82381 ggcattccga ccatcggttt gccgggctat tctgccccat ctgcgggaac ggcggccctg
    82441 aatcattccg gaaaagttga tacgcataac ttttacggca cggattccga ttacgacgat
    82501 tcgaccaccg acaccgctac catgcgtttt gagcacgaca tcaacgataa caccaccatt
    82561 cgcaatacta cccgttggtc gcgcgtgaag caggattacc tgatgacggc gattatgggt
    82621 ggggcgtcga atattaccca gcccaccagc gatgtgaata gctggacctg gtcacgcacg
    82681 gcgaatacta aagatgtgag taataaaatt ctcaccaacc agaccaacct gacctcgacg
    82741 ttctataccg cttctatcgg tcatgatgtc agtaccggcg tggaatttac ccgtgaaacg
    82801 cagactaact acggcgttaa tccggtgacg ttacctgcgg taaatattta tcatcctgac
    82861 agcagcattc atcccggcgg cctgacgcgc aacggcgcaa acgccaatgg tcagacggat
    82921 accttcgcaa tttacgcctt cgatacgctg caaatcaccc gtgattttga gctgaacggc
    82981 gggatccgtc tggataatta tcatactgaa tatgacagtg ccaccgcctg cggcggcagc
    83041 ggacgcggtg ccatcacctg cccagctggt gtggcaaaag gttctccggt caccaccgtc
    83101 gacaccgcca agtcgggcaa tctggtgaac tggaaagccg gggcgctgta tcacctgacg
    83161 gaaaacggta atgtctatat taactatgcc gtttcccagc agcctccggg cggcaacaac
    83221 ttcgcccttg cgcagtctgg cagcggtaac agtgccaacc gcaccgattt taaaccgcaa
    83281 aaagccaaca ccagcgagat tggcaccaaa tggcaggttc tggataaacg cctgttgctc
    83341 accgccgcgc tgttccgtac tgatatcgaa aatgaagttg agcaaaatga tgacggaact
    83401 tactcgcaat acggtaagaa acgcgtcgaa ggctatgaga tatccgtggc cgggaatatc
    83461 actcccgcgt ggcaggtgat tggcggctat acccagcaaa aagcaaccat caaaaacggc
    83521 aaagatgttg cccaggatgg ttcctcatcg ctgccgtata ccccggagca cgcctttacc
    83581 ttatggagcc agtatcaggc aaccgatgat atttccgttg gtgcgggcgc acgctatatc
    83641 ggcagtatgc ataaaggttc tgacggcgcg gtgggaacgc cagcgtttac cgaaggttat
    83701 tgggtcgccg acgctaaact ggggtatcgg gttaatcgca atctcgactt ccagctaaac
    83761 gtctacaacc tgtttgatac cgattacgtc gcctcaatca acaagagcgg ctaccgttat
    83821 cacccgggcg agccaagaac cttcttgctc acggccaata tgcatttctg attcagatgt
    83881 ggggcgcagg ccccactttt tggagaactt gtatgatgta ccacattccc ggcgtgctat
    83941 cgccaaagga cgtcgcccgt tttcgcgaac aactggaaca agccgaatgg gtggatggac
    84001 gcgtcaccac cggcgcacaa ggtgcgcaag ttaagaacaa tcaacaggta gacacccgca
    84061 gcacgttata cgccgccctg caaaatgagg tgctgaacgc ggttaaccaa catgctttgt
    84121 tctttgccgc ggccttgccg cgtacccttt ccacgccgct gtttaatcgc tatcagaaca
    84181 atgaaactta tggttttcat gtggatggcg cagtacgcag tcacccacaa aacggctgga
    84241 tgcgcactga cctttctgcc acgctgtttt taagcgatcc acaaagctac gatggcggcg
    84301 aactggtcgt taatgacacc ttcgggcaac atcgggtaaa acttccggca ggcgatctgg
    84361 tgttgtatcc ctccagcagc ttgcattgcg tgacacccgt aacccgcggc gtacgagtgg
    84421 catcatttat gtggatccag tcgatgatcc gtgatgataa aaagcgcgcc atgctgtttg
    84481 aactggacaa caatattcag tcgctgaaaa gccgctacgg cgaaagtgaa gagatcctgt
    84541 cgctgctcaa tctttatcat aatctgctgc gggaatggtc ggagatctga cgcttgaact
    84601 gcccgagttc gtccctatat tagctacact taactgtaca agtattgata tggggaggac
    84661 gatatggcat ccggttgggc taacgatgac gccgtcaacg aacagatcaa cagtacaatt
    84721 gaagatgcga ttgcccgcgc tcggggtgaa attccgcgtg gcgaaagcct gtatgaatgt
    84781 gaagagtgcg gagcccccat cccgcaagcc cgtcgggaag ccattcctgg tgtgcgctta
    84841 tgcattcatt gtcagcagga gaaagattta caaaaaccag cttatacagg atataatcgc
    84901 agaggttcga aagacagcca gttacgttaa ctacaactgg cgggatgtat agcgagtata
    84961 atttgtattt tgcgtggcat tccgtgcctt taacgctgtc gtgactgcca cgctgcataa
    85021 aacaagtcca taagttcaac aaataaatgg attatcagta tgttagatat caatcaattt
    85081 atttgaacaa ggcggtcaat tctcttcgat tttatctctc gaaaaaaaac gtgatactca
    85141 tcacatcgac gaaacaacgt cacttataca aaaatcacct gcgagagatt aattatgaaa
    85201 actatcaata ctgttgttgc tgctatggct ctttcaactc tgtcatttgg cgtgtttgcc
    85261 gcggaaccgg taacggcatc ccaggcacag aacatgaata aaatcggcgt ggtttctgcc
    85321 gatggcgcat ccaccctcga tgccctggaa gcgaaactgg ctgagaaagc tgcggcagct
    85381 ggggcgagtg gatacagcat cacttctgcc accaacaaca ataaattaag cggtactgcg
    85441 gtaatttata agtaattcgt aagccggatc aggcgtttac gctacgtcca gcacaagtat
    85501 ttccctgtct gtttgccgac agacgcatat gctctaaccc tcattgatcc tatgttaccc
    85561 ttgtttgccc gtccgccact ggacgggctt ttttttagct ggctaactgc tgacagaaag
    85621 cctgcaacgt ctcttccggc ataccaacct ttcgcgccgc atcacaaatg gcctgccagc
    85681 ttcccgcatc caatggaatc ccctgctcct gccgttcgcg acgcgtgtta acttcccact
    85741 cgcccggtag caaaatcggc ttatcgtcat catgcggcga ggctttcacc cactcggcaa
    85801 aggcttcggt ctgcgcgcta caatccggcg cgccgaacag ttccgggttg atgatgatag
    85861 tggtcatgca gttaagaatg gcatcgggac tggtttgtaa cgttttctga tgcgtcgttt
    85921 taccgccgga aagcgcccca ccaagaattt cacacatcgc tgcaagggcg tagcctttat
    85981 gttcggcaaa ggtcaacagc gaacccaacg gcgactcctg cattaccgcc ggattggtcg
    86041 tcggcatgcc gttaacgtca atcaggcaac ctggcggcac ggggacgcct ttatgccagg
    86101 cgacgcgggt tttgccaaat gcgatggcgc tggtggcgta atcaagcaac agcgggaaat
    86161 tatctttacg agggaaaacc acacagaacg gattggtgcc aaagcggctg tcgcgaccgt
    86221 ggaacggcgc gaccattgga ataccgacca cgctaacaaa gtggatagag acaaaccccg
    86281 ccgctgcaca ctgctccgcc cagtaaccga tacggccgat atgatgcgag ttatggagcg
    86341 ccacggcggc aatgccgtgc tgatgcgctt tctcaatccc cagcgccatc gcttcatgtg
    86401 ccgcgacctg accaaatgcg cgatcgccat cgagcgtgac cgccgccccc gcctctttaa
    86461 cgattttggc atgatggtta atttgcaggt gcccctgact ccaggagcgc acatagcttg
    86521 ggatcatgcc aataccatgt gaatcatgcc ctgccaggtt tgccgcgatt aaatgatcgg
    86581 caactaattt cgcttcttgt tcctcactac ccatctgacg aaatacagcc tgaataaaac
    86641 tgtgcagcgt ctgagcatca aagcgatgac cactttccat ggttaactcc tgtttatgtt
    86701 atgtgtttgt tgttttttta tgctgcacgc cgggcgcggg aaggtcaata aagatgcagg
    86761 aatttcatat tgtggtgggg taaaagccgg atgacatggc tcatccggct gagatgaaaa
    86821 ttaaaatgcc tgattaacac gcgccaggcc gtcgctgata gttgccgctt cccccgtagc
    86881 caccaggcag caagccatct ggattttcag cgattcggga atcggttcgc tgccagccag
    86941 gcaacgctca atccactgcg ccgtggtttc cggatctttt gcttgtggca gtaactcgct
    87001 accagcaggg tcctgttttt catacagcac ccgcatccct tcacggtcaa tgagattgat
    87061 ctgcgggcag cgctgcggat tagcatacac ttcgccttca gtaccatgca ttagtaacgc
    87121 ccgcccgccg atatcgctaa agaacttcgc gactcgcccg atgtattccg gatgcgaaac
    87181 gctggaaaga cgcagtgcct caccttcggc aaatggcgtc gccagtttcg ccagggtatg
    87241 tgcactgtta cgcacgccca tccgccagcg catcgccagt tgtttttcca gcggcgggca
    87301 aaacgcgccg acaggcataa acaccggttg atgttcgtcg agcttcgcct gcgcctgccc
    87361 gccgtgcaac gttggcgtaa tacccatcag ttcgaaaatg gtttctgtca gcacgcgggt
    87421 tggatcttcg ctaaccccgt gaaccaccac aggaaaaccg agtttgtgca ggagaatcgc
    87481 caacaacggc gtcaggttgg cctgtttacg cgcgccgttg taactgggga tgacaatcgg
    87541 catcggcttg tctgcaggcg gcgtcagctt gatggtgtga ttttgcatgg cttcgtaaaa
    87601 gccgagcatc tctgcttccc cttccccttt gatacgcagc gcaatcaata cgccgcccaa
    87661 ctcaaggtca gggacttcac cattgagcat atgggcatac agaccgcgcg cagtatcccg
    87721 gtctaaatcg cgcgcgtggt ttttcccgcg cccgatctct ttaatgattt tgcgatagtc
    87781 catttacgac tccttacctg actcacatca ttaacgccgc cgacggcgtg gggatttcgt
    87841 tttttctttc tttttaacta taccttcagg gacttccggt tgctctatcg gaaataccgg
    87901 taatgcatcc agtagccgct tgccgtagtt tttggtgagc aagcgtttat cgtaaatcac
    87961 cacttcgccc cagcaaccgt ggcttcgaat cagtcgccca acctgctgaa tcaggttaaa
    88021 cgaggcgctc ggcaggcttt gcacctcaaa cggatagcgg ttgaggcttt tcagccattc
    88081 cccttcggtg atcaccacgg ggctgtcgat gggcggaaaa gcgattttgt ggatatgcac
    88141 ctggctgagc agatcgcctt tcagatcaag cccttcggca aatgactgta agcccaccag
    88201 cacgctgcgc tcaccgttgc cgacgcgttt gcggtgcagt tcaactaaac ggtaacgcgg
    88261 ctgatcgccc tgcaccagca acatcaggcg taaatccgtc acatagtcga gaaaccgctg
    88321 catcgcccgt ccgctggcaa acagtaccaa cataccgaga tgttttttgc tctccacctg
    88381 ctcacggaaa aaggctgcca tttcagcaat gtgctgctct tcgttgtcga tggaaggctc
    88441 aaagcgcatc cggggaataa caattttgcc ctgttcgcag tggttaaagg gcgaatccag
    88501 cgccacaaag cggtcgcccg ctttctcttt cagaccactc atctcctgca aacgcgaaaa
    88561 actgttcagc gaacgtaagg tcgcggaagt gacaataatg tgcggaatac tgcgccacag
    88621 cagcctttcc agctgatcgc tgacacgaat acccacacag tgaaaccaga gatgcagctg
    88681 cccttcgcgc tcttcccgcg tcgcccattt ggtcaccggc gcaccggaag attgcgccag
    88741 agacgccagc cgccagagtt tgctttgcgc ctcgaacatc cccagcgcgc gattcatctg
    88801 caaaatcaac cgatgcagac gtacaatgtc atggctgccg gttttctcac ttaaatcgtt
    88861 aagaaataac tccgccaggc cacgcagcat ctcggtgagt tttgccagcc gctggcagat
    88921 ctccagcact tcatctagca gttcgcccat cgcaaaacgg tgctctgcct cctgcccggc
    88981 aggcatgtag agattgagaa tgttgtttaa cgaggcgata agctcataca actcttcaca
    89041 atgcgcattc aaacgttcag ggatcgccag cggcgggatg gtcttcgggc gaaactgctc
    89101 catgcaggta gcgaccagtt tcgtgaacaa gtccaactgt agtcgatacc acggcgcggt
    89161 gatttcggca ctcatctcca gcgcatcccg cgccacatct ggcaggtgat gaccttcgtc
    89221 cagcaccagc agcaaatttt tcgggtcagg caatacggct tcgctttcca tcgccgccat
    89281 caccagcgca tggtttgcca ccaccacttc cgcttcctga atctcccgac gagcgacaaa
    89341 aaacgggcat tcgcgatagt aataacagtt gcggttgagg cagctggctt tgtcggtact
    89401 taaacgacgc cagagatcgt catctatagc gatatcagta tgatcgcgca gaccatccca
    89461 tttataagtg tcgagatcgc ccttcagctt cgcacaacgt ttttgctctt cctgattatt
    89521 tggcgtcagt tcgtcgtcaa gaaacgccag cagatcctgt tgcgtgggtt cagtgctggc
    89581 gagcgccgtc agattacgcg ggcaaacgta gcgcccacgc ccaaaagcgg cagtgaattt
    89641 aagatcggga atgatctttt tcagcagcgg taaatctttg ctgtagatct gatcctgcaa
    89701 tgccacgttg gcggtactca ccaccagcgt tttttgctct tcgcgggcaa tggcgatgcc
    89761 gggaatcaaa taagagagcg ttttcccaac gccggtgggg gcttcaatcg ccagatgccg
    89821 cccttcttct ccggccagcg ttttggcgac gtccgcaatc atctgccgct gcggcgcacg
    89881 gggaataaag tcggggatct gttcctgaag cgccttatac caggcggcaa tttgcgcttt
    89941 aagcgcggcg gttaatgcca tgagaaaacc tgaaatactg tataaacagc caatattgtg
    90001 gcatttttcg gttttcaggg gtaatggttt ttctgtttct ggaaagcggc tcgcaataac
    90061 gttttccgga tgtgaagttt tctcgcggca gaagaaacat cattgtgcag caaaagacat
    90121 tgtacatcag cttattccgc tggcggccat tgcagagcgg tatgtaaaac acgcagtacc
    90181 gagagcgtat cgccctccac ttgatagaca agcacataat gcggcctgac aacggcttca
    90241 tgtgtaccag cgactcgtcc ggcccggtac atctccgggc gttcacaggc ctgttccgca
    90301 atggcttcaa attgattatc aagggctatt gccgctagcg gattatcttt actgatgtat
    90361 tccatgattt gctccctgtc agacagggac attggcttcc attccagacg cattaatccc
    90421 ccttcagcaa ggctttttta cgggcagcaa atagactttt ggcctcttca tgagagagtg
    90481 ccgggcgtgg atcgtcaatg gcggcctgca cctgctctcg aaaccaggca tcatacgcgg
    90541 cagcttcatg agctcgtttt aatgcctcag ccctgtctgg tcgggtcgtt ttcttctgac
    90601 cgtccgggtc gtaattagag gcgtcgactt caaaccgggc aataccaaca tctttcaggt
    90661 agttgacaac cgtttccagc cgtttgaaca ggcggatttg ccgactgcgt tgtgctgcaa
    90721 gaggccgttc agtgtggccg tatttcacgg tcaacgacca gcctccctgg gtgccaataa
    90781 cgtgtgctgc atccactacg ccagattcaa ccagtttatg taaggttgag tgatcgatcg
    90841 tttctggtgc cataatgcgc taacctcttt gtatacagca tgcaatttat aattaaagat
    90901 agagcaatta ataataaatt gcagcacctt gatcgtaaat tgcccaggat aaaattgcgg
    90961 tagaaagaga cttgttgcga agatgccggg catcgcccgg cataaaagat tactgcgcag
    91021 cggcaggttt acgcggacgg cggcgacgtt gttgttcgcc tgccggtttg gcatcgccga
    91081 gacgacgaga cggtttttct gcgggcttcg cgttaccaga cttcgcacca ctttccgcac
    91141 ggcgtggttg ctgttgaccg cgaccaccac cttgcccacg accgccgccg ccacgttgct
    91201 ggcgaccgtt ctggatcggt tcggctttga ttgacgggtc cggctcataa cccggaatcg
    91261 caatgcgcgg gatctctttt ttcagcagtt tttcgatatc acgcagcagt ttgtgttcat
    91321 caacacacac cagcgacaac gcttcaccgg tagcagccgc acgaccggta cgcccgatac
    91381 ggtggacata atcttccggt acgtttggca gttcatagtt gaccacgtgc ggcagctctt
    91441 caatatccag gccgcgcgca gcgatgtcag ttgccaccag tacacgaata tcgcccgatt
    91501 taaaatcagc cagcgcacgg gtacgcgcac cttgcgattt attgccgtgg atcgccgcac
    91561 tacggatgcc atctttattg agctgttcag ccagatggtt agcgccatgt ttggtacggg
    91621 taaacaccag cacctgctgc cagttccctt tcccaatcat gtgcgacaac aattcgcgtt
    91681 tgcgtttctt atcaacaaag tgaacatgct gggtcacctg atcagacgcg gtattgcggc
    91741 gtgccacttc gatttccagc gggttgtgca acagtttttc cgccagagct ttaatatcgt
    91801 cagagaaggt cgcggagaac aacaggttct ggcgcttcgc aggtagtttt gttaacacgc
    91861 gacggatatc gtggataaag cccatgtcga gcatgcggtc agcttcatcg aggacgagga
    91921 tttcaacctg atccagcttc accgcattct gatgttccag gtccagcaga cgtcccggcg
    91981 ttgcaaccag cacatcaacg ccgccgcgca acttcatcat ctgcgggtta atgctgacac
    92041 cgccaaacac caccagcgaa cgaatgttca ggtatttgct gtaatcacgg acgttttcgc
    92101 caatctgcgc cgccagttca cgggtcgggg taagaatgag cgcacgtacc ggacgacgcc
    92161 ctttggcgtg cggctggcga gtgatcaggt gttgcaacag cggcagcgta aagcccgctg
    92221 ttttgccggt gccagtctgg gcgctagcca tcaggtcgcg gccttccagc accgcaggga
    92281 tcgcctgctg ctgaataggg gtgggttcac ggtaaccctg ctcggcaacg gcgcgcagga
    92341 tatcagggct taaacccaaa gaatcgaaag acataactac tccgaaccgc cccgaccgtt
    92401 accggtgtag ttttcaggga gatactgaaa aggaaaatga caaaaaccac aatgtcatga
    92461 gggagcggag tgtagcagtt tttgtgacgc agcgcataaa ttatccctgt gactggcgtt
    92521 gctaaaattc ttagccatac tggacaactc ccatatggag tcataatctt aatcaatcat
    92581 ttgattaaga atggactgtg cgatgaataa tcctgccatg acaatcaagg gtgaacaggc
    92641 gaaaaaacag ctgattgctg ccgcactggc gcagtttggt gaatatggaa tgaacgccac
    92701 cacccgcgag atagccgccc aggccgggca gaatatcgct gccatcacct actacttcgg
    92761 ttcgaaagag gatttgtacc tcgcctgcgc ccagtggatt gccgatttta ttggcgagca
    92821 gttccgtccg cacgccgaag aggccgaacg cttgttcgca caaccacagc cagatcgggc
    92881 tgccatccgt gaactgatcc ttcgcgcctg caggaacatg attaagctgc tcacccagga
    92941 tgacactgtc aacctcagca aatttatctc ccgtgagcag ctctctccca cggcagccta
    93001 tcacctggtg cacgaacagg tgattagtcc gctacacagc caccttacac gcctgattgc
    93061 cgcctggacc ggctgcgacg ccaatgatac ccgcatgatc cttcataccc atgcgttgat
    93121 tggtgagatt ctggcgtttc gtcttggcaa agaaacaatt ctgttacgta ccggctggac
    93181 cgcgttcgat gaagaaaaga ccgaactgat taaccagacg gtgacctgtc atatcgacct
    93241 gattttgcaa ggattatcgc aaaggagttt gtagtgatga aaaaacctgt cgtgatcgga
    93301 ctggcggtag tagtacttgc cgccgtggtt gccggaggct actggtggta tcaaagccgc
    93361 caggataacg gcctgacgct gtatggcaac gtggatattc gtacggtgaa tcttagtttc
    93421 cgtgttgggg ggcgcgttga gtcgctggca gtggacgaag gtgatgcgat caaagcgggc
    93481 caggtgctgg gcgaactgga tcacaagccg tatgagattg ccctgatgca ggcgaaagcg
    93541 ggcgtttcgg tggctcaggc gcagtatgac ctgatgcttg ccgggtatcg cgatgaagaa
    93601 atcgctcagg ccgccgcagc ggtgaaacag gcgcaagctg cctatgacta tgcgcagaac
    93661 ttctataacc gccagcaagg actgtggaaa agccgcacca tttcggcaaa tgacctggaa
    93721 aatgcccgct cctcgcgcga ccaggcgcag gcaacgctga aatcggcaca ggataaattg
    93781 cgtcagtacc gttccggtaa ccgcgaacag gatatcgccc aggcaaaagc cagcctcgaa
    93841 caggcgcagg cgcaactggc gcaggcggag ctgaatttgc aggactcaac gttgatagcc
    93901 ccgtctgatg gcacgctgtt aacgcgcgcg gtggagccag gcacggttct caatgaaggt
    93961 ggcacggtgt ttaccgtctc gctaacacgt ccagtgtggg tgcgtgccta tgttgacgaa
    94021 cgtaatcttg accaggccca gccggggcgc aaagtgctgc tttataccga tggtcgcccg
    94081 gacaagccgt atcacgggca gattggtttc gtttcaccga ctgctgaatt taccccgaaa
    94141 accgtcgaaa cgccggatct gcgtaccgac ctcgtctatc gcctgcgtat tgtggtgacc
    94201 gacgccgatg atgcgttacg ccagggaatg ccagtgacgg tacaattcgg tgacgaggca
    94261 ggacatgaat gatgccgtta tcacgctgaa cggcctggaa aaacgctttc cgggcatgaa
    94321 caagcccgcc gtcgcgccgc tcgattgcac cattcatgcc ggttatgtga cggggctggt
    94381 agggccagac ggtgcgggta aaaccacgct gatgcggatg ctggcgggat tactgaaacc
    94441 cgacagcggc agtgccacgg tgattggctt tgatccgatc aaaaatgacg gcgcgctgca
    94501 cgccgtgctc ggctatatgc cgcagaaatt tggtctgtat gaagatctga cggtgatgga
    94561 gaacctcaat ctttacgcgg atttgcgcag cgtcaccggc gaggcacgga agcagacttt
    94621 ttctcgcctg ctggagttta cgtctcttgg cccgtttacc ggacgcctgg cgggcaagct
    94681 ctccggtggg atgaaacaaa aactcggcct ggcctgtacc ctggtgggcg aaccgaaagt
    94741 gttgctgctc gatgaacccg gcgtcggcgt tgaccctatc tcacggcgcg aactgtggca
    94801 gatggtgcat gagctggcgg gcgaagggat gttaattctc tggagcacct cgtatctcga
    94861 cgaagccgag cagtgccgtg acgtgttact gatgaacgaa ggcgaattgt tgtaccaggg
    94921 agaaccgacg gccctgacac aaaccatggc cggacgcagc tttctgatga ccagcccgca
    94981 cgagggcaac cgcaaactgt tgcaacgggc attgaaactg ccgcaagtca gcgacggcat
    95041 gattcagggg aaatcggtac gtctgatcct caaaaaagag gccacaccag acgatattcg
    95101 ccatgccgac gggatgccgg aaatcaacat caacgaaact acgccgcgtt ttgaagatgc
    95161 gtttattgat ttgctgggcg gtgccggaac ctcggaatcg ccgctgggtg caatattgca
    95221 tacggtagaa ggcacacccg gtgagacggt gatcgaagcg aaagaactga ccaagaaatt
    95281 tggtgatttt gccgccaccg atcacgtcaa ctttgccgtt aaacgcgggg agatttttgg
    95341 tttgctgggg ccaaacggcg cgggcaaatc gaccaccttt aagatgatgt gcggtttgct
    95401 ggtaccgacc tctggccagg cgctggtgct gggcatggat ctgaaagaga gttccggtaa
    95461 agcgcgccag catctcggct atatggcgca aaaattttcg ctctacggca acctgacggt
    95521 cgaacagaat ttacgctttt tctctggtgt gtatggctta cgcggtcggg cgcagaacga
    95581 aaaaatctcc cgtatgagcg aggcgttcgg cctgaaaagt atcgcctccc acgcgaccga
    95641 tgaactgcca ttaggtttta aacagcggct ggcactggcc tgctcgctga tgcatgaacc
    95701 ggacattctg tttctcgacg aaccgacgtc cggcgttgat cccatcaccc gccgtgaatt
    95761 ttggctacat atcaacagta tggtagagaa aggcgtcacg gtgatggtca ccacccactt
    95821 tatggatgaa gcggaatatt gcgaccgcat cggcctggtg taccgcggga aattaatcgc
    95881 cagcggcaca ccggacgatt tgaaagcgca gtcggccaac gatgagcaac ccgatcccac
    95941 tatggagcaa gcctttattc agttgatcca cgactgggat aaggagcata gcaatgagta
    96001 atccgatcct gtcctggcgt cgcgtgcggg cgctgtgcgt caaagagaca cggcagatcg
    96061 ttcgcgatcc gagtagctgg ctgattgcgg tagtgatccc tctgctgctg ctgtttattt
    96121 ttggctacgg cattaacctc gactccagca agctgcgggt cgggatattg ctggaacagc
    96181 gtagcgaagc ggcgctggat ttcacccaca ccatgacagg ttcgccctac atcgacgcca
    96241 ccatcagcga taaccgtcag gaactgatcg ccaaaatgca ggcggggaaa attcgcggtc
    96301 tggtggttat tccggtggat ttcgccgaac agatggagcg cgccaacgcc accgcaccga
    96361 ttcaggtgat caccgacggc agtgagccga ataccgctaa ctttgtgcag gggtatgtcg
    96421 aagggatctg gcagatctgg caaatgcagc gagcggagga caacgggcag acttttgaac
    96481 cgcttattga tgtacaaacc cgctactggt ttaacccggc ggcgattagc cagcacttca
    96541 ttatccccgg tgcggtgacc atcatcatga cggtcatcgg cgcgattctc acctcgctgg
    96601 tggtggcgcg agaatgggaa cgcggcacca tggaggctct gctctctacg gagattaccc
    96661 gcacggaact gctgctgtgt aagctcatac cctactattt cctcgggatg ctggcgatgt
    96721 tgctgtgtat gctggtgtcg gtgtttattc tcggcgtgcc gtatcgcggg tcgctgctga
    96781 ttctgttttt tatctccagc ctgtttttac tcagtaccct ggggatgggg ctgcttattt
    96841 ccacgattac ccgcaaccag ttcaatgccg ctcaggtcgc cctgaacgcg gcttttctgc
    96901 cgtcgattat gctttccggc tttatttttc agatcgacag tatgcccgcg gtgatccgcg
    96961 cggtgacgta cattattccc gcacgttatt tcgtcagcac cctgcaaagc ctgtttctcg
    97021 ccgggaatat tccggtggtg ctggtggtga acgtactgtt tttgatcgct tcggcggtga
    97081 tgtttatcgg cctgacgtgg ctgaaaacca aacgtcggct ggattaggga gaagagcatg
    97141 tttcatcgct tatggacgtt aatccgcaaa gagttgcagt cgctgctgcg cgaaccgcaa
    97201 acccgtgcga ttcttatttt acccgtgctg attcaggtga tcctgttccc gttcgccgcc
    97261 acgctggaag tgactaacgc caccatcgcc atctacgatg aagataacgg cgagcattcg
    97321 gtggagctga cccaacgttt tgcccgcgcc agcgccttta ctcatgtgct gctgctgaaa
    97381 agcccacagg agatccgccc aaccatcgac acacaaaagg cgttactact ggtgcgtttc
    97441 ccggctgact tctcgcgcaa actggatacc ttccagaccg cgccgttgca gttgatcctc
    97501 gacgggcgta actccaacag tgcgcaaatt gccgccaact acctgcaaca gatcgtcaaa
    97561 aattatcagc aggagctgct ggaaggaaaa ccgaaaccta acaacagcga gctggtggta
    97621 cgcaactggt ataactcgaa tctcgactac aaatggtttg tggtgccgtc gctgatcgcc
    97681 atgatcacca ctatcggcgt aatgatcgtc acttcacttt ctgttgcccg cgaacgtgaa
    97741 caaggtacgc tcgatcagct actggtttcg ccgctcacca cctggcagat cttcatcggc
    97801 aaggccgtac cggcattaat tgtcgccacc ttccaggcca ccattgtgct ggcgattggt
    97861 atctgggcgt atcaaatccc cttcgccgga tcgctggcgc tgttctactt tacgatggtg
    97921 atttacggtt tatcgctggt gggattcggt ctgttgattt catcactctg ctcgacacaa
    97981 cagcaggcgt ttatcggcgt gtttgttttt atgatgcccg ccattctcct ttccggttac
    98041 gtttctccgg tggaaaacat gccagtatgg ctgcaaaacc tgacgtggat taaccctatt
    98101 cgccacttta cggacattac caagcagatt tatttgaagg atgcgagtct ggatattgtg
    98161 tggaatagtt tgtggccgct actggtgata acggccacga cagggtcagc ggcgtacgcg
    98221 atgtttagac gtaaggtgat gtaacttctt atctttcgcc agcaaagaca ccaccgccgg
    98281 gccggcaagg attgccagcc ctgcaatcgc cagcaaaaag ttgttttcca gtacacggga
    98341 caatagccac agcacaatca gcgctgcgcc aaaataccag gtcgtggtca gctcttccag
    98401 cacgtctgcc acctcacgcc agcgttgctc ggggtggcgc tgaaacacca gcatcatcac
    98461 taccgtgaca caatacagtg cgcataatcc cacgccgacg tgaaccagag tcttactcat
    98521 ccagcccacc accagcagcg ccactaccag taaatgcaac ataatctgcc agcaacttag
    98581 acccgttgcg acacgaacac gttgttgcca cttcatggct tctctcctga agaatttatc
    98641 tctacttact cagagtaccg aactttacgg aaaattccgt aagaccgcac ctttttgtga
    98701 cgccgactac actatttcaa tgagataaga ggcaaagaca ggaaaattat gcccgatcaa
    98761 acacaacaat tttcgttcaa ggtgctcacc atcaatattc acaaaggctt taccgcgttt
    98821 aaccgacgct tcattttgcc ggaacttcgc gacgccgtgc gtaccgtcag cgccgatatt
    98881 gtttgcctgc aggaagtgat gggcgcgcac gaagttcatc cgctgcatgt ggaaaactgg
    98941 cccgatacct cgcactacga gtttctcgcc gacactatgt ggagcgattt tgcctacggt
    99001 cgcaatgccg tatacccgga agggcatcac ggtaacgccg tactgtcgcg ctatcccatt
    99061 gaacattatg aaaaccgcga tgtttcggtc gatggtgcgg aaaagcgcgg cgtgctctac
    99121 tgccgcattg tgccgccgat gaccggaaaa gcgattcatg taatgtgcgt acatttgggc
    99181 ctgcgtgagg cgcaccgtca ggcgcagctt gcgatgctcg ccgaatgggt gaatgagcta
    99241 ccggacggcg aaccggtatt ggtggcgggt gatttcaatg actggcggca aaaagctaat
    99301 catccgttaa aagtgcaggc cggactggat gagattttta cccgcgccca cggacgcccg
    99361 gcgcgcacgt ttccggtgca atttcctcta ctacgactgg acaggatcta cgtcaaaaat
    99421 gccagcgcca gcgcgccaac cgcgttgccg ctgcggacat ggcgacacct ttctgatcat
    99481 gcccctttaa gtgcggagat tcatttatga aatgtagctg gcgcgaaggc aataagatcc
    99541 agttgctgga aaacggcgag caatattatc ccgcggtgtt taaggcgatt ggcgaggcac
    99601 aagaacgcat cattcttgaa acgtttatct ggtttgagga tgacgtcggc aaacaactgc
    99661 atgcggcact actggcagca gcgcaacgcg gggtaaaagc ggaagtctta cttgatggct
    99721 acggttcgcc ggatctcagc gatgagtttg tcaatgaact gacggccgct ggcgtggtgt
    99781 tccgctacta cgatccccgc cctcgccttt ttggtatgcg caccaatgtg tttcgccgga
    99841 tgcatcgcaa aattgtggtg atcgacgcgc atatcgcctt tattggcggg ctgaattact
    99901 ccgccgagca tatgtccagc tacggtccag aggctaaaca ggattacgcg gtgcgccttg
    99961 aagggccgat tgtcgaagat attctgcaat ttgaactgga aaacctgcca ggacagagcg
   100021 cggcacgacg ctggtggcga cgtcatcaca aagcggaaga gaaccgccag ccgggagaag
   100081 cgcaggtatt gctggtctgg cgcgataacg aagaacatcg cgatgatatt gaacgtcact
   100141 atctgaaaat gctcactcag gcacggcggg aagtgattat cgccaacgcc tacttcttcc
   100201 ccggctatcg atttttacac gccttgcgta aagcggcacg gcgcggggtg cggatcaaac
   100261 tgatcattca gggcgaaccg gatatgccga ttgtcagagt cggcgcgcgc ttgctgtata
   100321 actatctggt taaaggcggc gttcaggttt ttgagtatcg ccgccgcccg ctccacggca
   100381 aagtggcgtt gatggacgat cactgggcga cggtagggtc cagtaatctc gatccgctca
   100441 gtttgtcact gaatctcgaa gcaaatgtca tcatccacga tcgtcatttt aaccagacgc
   100501 tgcgcgataa tctgaacggc attatcgccg cagactgcca gcaggtggat gaaaccatgc
   100561 tgccgaaacg cacctggtgg aatctgacca aaagcgtgct ggccttccac tttttacgcc
   100621 acttcccggc gctggtcggc tggcttccgg cacacacacc acgtctggcg caggttgacc
   100681 cgcccgctca gccgacaatg gaaacgcagg atcgggtaga aactgaaaac acgggggtaa
   100741 aaccctgatg agtaaatcac acccacgctg gcgcttagca aagaagatcc tcacctggct
   100801 gttttttatc gcggtgatag tgttgctggt ggtctacgcc aaaaaggtgg actgggaaga
   100861 ggtctggaag gtcatccgcg actacaatcg cgttgcgttg ctcagtgcgg tcgggctggt
   100921 ggtcgtcagt tatctgattt acggctgcta tgatctgctc gcccgctttt actgcggtca
   100981 caaactggcg aagcgccagg tgatgctggt gtcgtttatc tgctacgcct tcaacctgac
   101041 gctcagtacc tgggtcggcg gcattggtat gcgctatcgt ttgtactccc ggctagggtt
   101101 accgggcagc actattacgc ggattttctc gctcagtatt accaccaact ggctgggcta
   101161 tattttactg gcagggatta tctttaccgc aggcgtggtg gagttgccgg atcactggta
   101221 tgtcgatcaa accacgctgc gtattctcgg cattggctta ttgatgatta tcgcggttta
   101281 tttgtggttt tgcgctttcg cgaagcaccg ccatatgacc atcaaaggac aaaagctggt
   101341 gctgccttca tggaaatttg ccctcgccca gatgctgatt tccagcgtta actggatggt
   101401 aatgggggcg attatctggc tgttacttgg tcaaagcgtg aactatttct ttgtactggg
   101461 cgtgttactg gttagtagta ttgctggcgt catcgtgcat attccggcgg ggattggtgt
   101521 gctggaagcg gtatttatcg cgctactggc tggggagcat acctcgaaag ggagcattat
   101581 cgccgccctg ctcgcttatc gcgtcctgta ttactttatc ccgctgctgc tggcgctgat
   101641 ctgttacctg ctactggaaa gccaggcgaa gaagctacga gcgaaaaatg aagcggcgat
   101701 gtgaagttta gttaagttct ttagtatgtg catttacgac taatgaaaaa aacgcgtatg
   101761 cctttgccag acaagcgtta tagctatcgc ggcaagtatc ccaaagaagc cgataaaata
   101821 gtaatagagt gttaatgcac ctgtaaaagc gcaacgaacc ggactccaca actcttcgct
   101881 atggcatttt cctgccagtt caaggagctt atttcgtcca tgcgagataa tgcctgacca
   101941 taacactatc attaagcaac ttattaccca aacgggtcgt tcactcatta atgcggcatc
   102001 catgatgatc accagggcca gctctgcggt tgccaccata cccaaagcgg taatgagcca
   102061 tataccgttt aatatcctgg cctcgtgctg aaaatctaat ttattgctgt tttgcgagta
   102121 tgattccatt cttttagttg tccttagaaa aaacattaat attcaccatc aataaattta
   102181 aaaccacgca tacaaaatat ttttgtattt atgga
//
LOCUS       DAOTRK010000002        93461 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.002, whole genome shotgun sequence.
ACCESSION   DAOTRK010000002 DAOTRK010000000
VERSION     DAOTRK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.002"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..333)
                     /locus_tag="QIE41_000097"
     CDS             complement(<1..333)
                     /locus_tag="QIE41_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDS3529037.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFG"
     gene            485..814
                     /locus_tag="QIE41_000098"
     CDS             485..814
                     /locus_tag="QIE41_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDS3529038.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(871..2205)
                     /locus_tag="QIE41_000099"
     CDS             complement(871..2205)
                     /locus_tag="QIE41_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDS3529039.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEVALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITSGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVLPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEGMARQVAQQLRKALS"
     gene            2293..2724
                     /locus_tag="QIE41_000100"
     CDS             2293..2724
                     /locus_tag="QIE41_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDS3529040.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            2933..3178
                     /gene="nrdH"
                     /locus_tag="QIE41_000101"
     CDS             2933..3178
                     /gene="nrdH"
                     /locus_tag="QIE41_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDS3529041.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3175..3585
                     /gene="nrdI"
                     /locus_tag="QIE41_000102"
     CDS             3175..3585
                     /gene="nrdI"
                     /locus_tag="QIE41_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDS3529042.1"
                     /translation="MSQLVYFSSSSENTQRFIERLRLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRHPQNA"
     gene            3558..5702
                     /gene="nrdE"
                     /locus_tag="QIE41_000103"
     CDS             3558..5702
                     /gene="nrdE"
                     /locus_tag="QIE41_000103"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDS3529043.1"
                     /translation="MATTPAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     ITTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            5712..6671
                     /gene="nrdF"
                     /locus_tag="QIE41_000104"
     CDS             5712..6671
                     /gene="nrdF"
                     /locus_tag="QIE41_000104"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDS3529044.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLLPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7028..8230
                     /gene="proV"
                     /locus_tag="QIE41_000105"
     CDS             7028..8230
                     /gene="proV"
                     /locus_tag="QIE41_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDS3529045.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8223..9287
                     /gene="proW"
                     /locus_tag="QIE41_000106"
     CDS             8223..9287
                     /gene="proW"
                     /locus_tag="QIE41_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDS3529046.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9345..10337
                     /gene="proX"
                     /locus_tag="QIE41_000107"
     CDS             9345..10337
                     /gene="proX"
                     /locus_tag="QIE41_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDS3529047.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            10790..11974
                     /locus_tag="QIE41_000108"
     CDS             10790..11974
                     /locus_tag="QIE41_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529048.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12098..12835
                     /gene="ygaZ"
                     /locus_tag="QIE41_000109"
     CDS             12098..12835
                     /gene="ygaZ"
                     /locus_tag="QIE41_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDS3529049.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            12825..13160
                     /gene="ygaH"
                     /locus_tag="QIE41_000110"
     CDS             12825..13160
                     /gene="ygaH"
                     /locus_tag="QIE41_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDS3529050.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAIGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13251..13781
                     /gene="emrR"
                     /locus_tag="QIE41_000111"
     CDS             13251..13781
                     /gene="emrR"
                     /locus_tag="QIE41_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDS3529051.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            13908..15080
                     /gene="emrA"
                     /locus_tag="QIE41_000112"
     CDS             13908..15080
                     /gene="emrA"
                     /locus_tag="QIE41_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDS3529052.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15097..16635
                     /gene="emrB"
                     /locus_tag="QIE41_000113"
     CDS             15097..16635
                     /gene="emrB"
                     /locus_tag="QIE41_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDS3529053.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(16699..17214)
                     /gene="luxS"
                     /locus_tag="QIE41_000114"
     CDS             complement(16699..17214)
                     /gene="luxS"
                     /locus_tag="QIE41_000114"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDS3529054.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17364..18920)
                     /gene="gshA"
                     /locus_tag="QIE41_000115"
     CDS             complement(17364..18920)
                     /gene="gshA"
                     /locus_tag="QIE41_000115"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDS3529055.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDIAGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSKELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(18993..19421)
                     /locus_tag="QIE41_000116"
     CDS             complement(18993..19421)
                     /locus_tag="QIE41_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDS3529056.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19418..19984)
                     /gene="yqaB"
                     /locus_tag="QIE41_000117"
     CDS             complement(19418..19984)
                     /gene="yqaB"
                     /locus_tag="QIE41_000117"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDS3529057.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20132..20208)
                     /locus_tag="QIE41_000118"
     tRNA            complement(20132..20208)
                     /locus_tag="QIE41_000118"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20172..20174),aa:Arg,seq:acg)
     gene            complement(20311..20387)
                     /locus_tag="QIE41_000119"
     tRNA            complement(20311..20387)
                     /locus_tag="QIE41_000119"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20351..20353),aa:Arg,seq:acg)
     gene            complement(20450..20526)
                     /locus_tag="QIE41_000120"
     tRNA            complement(20450..20526)
                     /locus_tag="QIE41_000120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20490..20492),aa:Arg,seq:acg)
     gene            complement(20725..20801)
                     /locus_tag="QIE41_000121"
     tRNA            complement(20725..20801)
                     /locus_tag="QIE41_000121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20765..20767),aa:Arg,seq:acg)
     gene            complement(20805..20897)
                     /locus_tag="QIE41_000122"
     tRNA            complement(20805..20897)
                     /locus_tag="QIE41_000122"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20861..20863),aa:Ser,seq:gct)
     gene            complement(21214..21399)
                     /gene="csrA"
                     /locus_tag="QIE41_000123"
     CDS             complement(21214..21399)
                     /gene="csrA"
                     /locus_tag="QIE41_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDS3529058.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21634..24264)
                     /gene="alaS"
                     /locus_tag="QIE41_000124"
     CDS             complement(21634..24264)
                     /gene="alaS"
                     /locus_tag="QIE41_000124"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDS3529059.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24392..24892)
                     /gene="recX"
                     /locus_tag="QIE41_000125"
     CDS             complement(24392..24892)
                     /gene="recX"
                     /locus_tag="QIE41_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDS3529060.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(24961..26022)
                     /gene="recA"
                     /locus_tag="QIE41_000126"
     CDS             complement(24961..26022)
                     /gene="recA"
                     /locus_tag="QIE41_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDS3529061.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26102..26599)
                     /gene="pncC"
                     /locus_tag="QIE41_000127"
     CDS             complement(26102..26599)
                     /gene="pncC"
                     /locus_tag="QIE41_000127"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDS3529062.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(26744..27829)
                     /gene="mltB"
                     /locus_tag="QIE41_000128"
     CDS             complement(26744..27829)
                     /gene="mltB"
                     /locus_tag="QIE41_000128"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDS3529063.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28085..28648
                     /gene="srlA"
                     /locus_tag="QIE41_000129"
                     /gene_synonym="gutA"
     CDS             28085..28648
                     /gene="srlA"
                     /locus_tag="QIE41_000129"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDS3529064.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            28645..29604
                     /gene="srlE"
                     /locus_tag="QIE41_000130"
                     /gene_synonym="gutE"
     CDS             28645..29604
                     /gene="srlE"
                     /locus_tag="QIE41_000130"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDS3529065.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            29615..29986
                     /gene="srlB"
                     /locus_tag="QIE41_000131"
                     /gene_synonym="gutB"
     CDS             29615..29986
                     /gene="srlB"
                     /locus_tag="QIE41_000131"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDS3529066.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            29990..30769
                     /gene="srlD"
                     /locus_tag="QIE41_000132"
     CDS             29990..30769
                     /gene="srlD"
                     /locus_tag="QIE41_000132"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDS3529067.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            30875..31234
                     /gene="gutM"
                     /locus_tag="QIE41_000133"
     CDS             30875..31234
                     /gene="gutM"
                     /locus_tag="QIE41_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDS3529068.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            31301..32074
                     /gene="srlR"
                     /locus_tag="QIE41_000134"
                     /gene_synonym="gutR"
     CDS             31301..32074
                     /gene="srlR"
                     /locus_tag="QIE41_000134"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDS3529069.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            32067..33032
                     /gene="gutQ"
                     /locus_tag="QIE41_000135"
     CDS             32067..33032
                     /gene="gutQ"
                     /locus_tag="QIE41_000135"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDS3529070.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33029..34543)
                     /gene="norR"
                     /locus_tag="QIE41_000136"
     CDS             complement(33029..34543)
                     /gene="norR"
                     /locus_tag="QIE41_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDS3529071.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            34730..36169
                     /gene="norV"
                     /locus_tag="QIE41_000137"
     CDS             34730..36169
                     /gene="norV"
                     /locus_tag="QIE41_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDS3529072.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSANTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36166..37299
                     /gene="norW"
                     /locus_tag="QIE41_000138"
     CDS             36166..37299
                     /gene="norW"
                     /locus_tag="QIE41_000138"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDS3529073.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEIGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCTE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(37377..39617)
                     /gene="hypF"
                     /locus_tag="QIE41_000139"
     CDS             complement(37377..39617)
                     /gene="hypF"
                     /locus_tag="QIE41_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDS3529074.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAASLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TTSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RILHAA"
     gene            complement(39770..40297)
                     /gene="hydN"
                     /locus_tag="QIE41_000140"
     CDS             complement(39770..40297)
                     /gene="hydN"
                     /locus_tag="QIE41_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDS3529075.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40446..41456)
                     /gene="ascG"
                     /locus_tag="QIE41_000141"
     CDS             complement(40446..41456)
                     /gene="ascG"
                     /locus_tag="QIE41_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDS3529076.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHSIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SQ"
     gene            41716..43173
                     /gene="ascF"
                     /locus_tag="QIE41_000142"
     CDS             41716..43173
                     /gene="ascF"
                     /locus_tag="QIE41_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDS3529077.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            43182..44606
                     /gene="ascB"
                     /locus_tag="QIE41_000143"
     CDS             43182..44606
                     /gene="ascB"
                     /locus_tag="QIE41_000143"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDS3529078.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARICFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDVGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(44685..45104)
                     /locus_tag="QIE41_000144"
     CDS             complement(44685..45104)
                     /locus_tag="QIE41_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3529079.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(45101..45412)
                     /locus_tag="QIE41_000145"
     CDS             complement(45101..45412)
                     /locus_tag="QIE41_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HDS3529080.1"
                     /translation="MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(45579..46049)
                     /gene="hycI"
                     /locus_tag="QIE41_000146"
     CDS             complement(45579..46049)
                     /gene="hycI"
                     /locus_tag="QIE41_000146"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDS3529081.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(46042..46452)
                     /gene="hycH"
                     /locus_tag="QIE41_000147"
     CDS             complement(46042..46452)
                     /gene="hycH"
                     /locus_tag="QIE41_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDS3529082.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLTNAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46449..47216)
                     /gene="hycG"
                     /locus_tag="QIE41_000148"
     CDS             complement(46449..47216)
                     /gene="hycG"
                     /locus_tag="QIE41_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDS3529083.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47216..47758)
                     /gene="hycF"
                     /locus_tag="QIE41_000149"
     CDS             complement(47216..47758)
                     /gene="hycF"
                     /locus_tag="QIE41_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDS3529084.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47768..49477)
                     /gene="hycE"
                     /locus_tag="QIE41_000150"
     CDS             complement(47768..49477)
                     /gene="hycE"
                     /locus_tag="QIE41_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDS3529085.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(49495..50418)
                     /gene="hycD"
                     /locus_tag="QIE41_000151"
     CDS             complement(49495..50418)
                     /gene="hycD"
                     /locus_tag="QIE41_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDS3529086.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(50421..52247)
                     /gene="hycC"
                     /locus_tag="QIE41_000152"
     CDS             complement(50421..52247)
                     /gene="hycC"
                     /locus_tag="QIE41_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDS3529087.1"
                     /translation="MSAISLINSGVAWFAAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATSAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(52244..52855)
                     /gene="hycB"
                     /locus_tag="QIE41_000153"
     CDS             complement(52244..52855)
                     /gene="hycB"
                     /locus_tag="QIE41_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDS3529088.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(52980..53441)
                     /gene="hycA"
                     /locus_tag="QIE41_000154"
     CDS             complement(52980..53441)
                     /gene="hycA"
                     /locus_tag="QIE41_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDS3529089.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53653..54003
                     /gene="hypA"
                     /locus_tag="QIE41_000155"
     CDS             53653..54003
                     /gene="hypA"
                     /locus_tag="QIE41_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDS3529090.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            54007..54879
                     /gene="hypB"
                     /locus_tag="QIE41_000156"
     CDS             54007..54879
                     /gene="hypB"
                     /locus_tag="QIE41_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDS3529091.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54870..55142
                     /gene="hypC"
                     /locus_tag="QIE41_000157"
     CDS             54870..55142
                     /gene="hypC"
                     /locus_tag="QIE41_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDS3529092.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            55142..56263
                     /gene="hypD"
                     /locus_tag="QIE41_000158"
     CDS             55142..56263
                     /gene="hypD"
                     /locus_tag="QIE41_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDS3529093.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEEPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            56260..57270
                     /gene="hypE"
                     /locus_tag="QIE41_000159"
     CDS             56260..57270
                     /gene="hypE"
                     /locus_tag="QIE41_000159"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDS3529094.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57344..59421
                     /gene="flhA"
                     /locus_tag="QIE41_000160"
                     /pseudo
     CDS             57344..59421
                     /gene="flhA"
                     /locus_tag="QIE41_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            59466..59894
                     /locus_tag="QIE41_000161"
     CDS             59466..59894
                     /locus_tag="QIE41_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HDS3529095.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(59933..60277)
                     /locus_tag="QIE41_000162"
     CDS             complement(59933..60277)
                     /locus_tag="QIE41_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDS3529096.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYETLFTYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(60354..60488)
                     /locus_tag="QIE41_000163"
     CDS             complement(60354..60488)
                     /locus_tag="QIE41_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529097.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            60564..63125
                     /gene="mutS"
                     /locus_tag="QIE41_000164"
     CDS             60564..63125
                     /gene="mutS"
                     /locus_tag="QIE41_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDS3529098.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            63231..63887
                     /gene="pphB"
                     /locus_tag="QIE41_000165"
     CDS             63231..63887
                     /gene="pphB"
                     /locus_tag="QIE41_000165"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDS3529099.1"
                     /translation="MPSTRYQKINAHHYRYIWAVGDIHGDYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FELNETEQQEAINLLLKFHRLPHIIEITNDIIKYVIAHADYPGNEYLFGKEIAERELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(63938..64705)
                     /locus_tag="QIE41_000166"
     CDS             complement(63938..64705)
                     /locus_tag="QIE41_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDS3529100.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            64901..65809
                     /locus_tag="QIE41_000167"
     CDS             64901..65809
                     /locus_tag="QIE41_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDS3529101.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            65806..67068
                     /locus_tag="QIE41_000168"
     CDS             65806..67068
                     /locus_tag="QIE41_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDS3529102.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            67065..67703
                     /locus_tag="QIE41_000169"
     CDS             67065..67703
                     /locus_tag="QIE41_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDS3529103.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            67708..68484
                     /locus_tag="QIE41_000170"
     CDS             67708..68484
                     /locus_tag="QIE41_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDS3529104.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            68573..69937
                     /locus_tag="QIE41_000171"
     CDS             68573..69937
                     /locus_tag="QIE41_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDS3529105.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(69976..70212)
                     /locus_tag="QIE41_000172"
     CDS             complement(69976..70212)
                     /locus_tag="QIE41_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529106.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(70223..71650)
                     /locus_tag="QIE41_000173"
     CDS             complement(70223..71650)
                     /locus_tag="QIE41_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDS3529107.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     VAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(71650..72243)
                     /locus_tag="QIE41_000174"
     CDS             complement(71650..72243)
                     /locus_tag="QIE41_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDS3529108.1"
                     /translation="MRLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYNARDVAALADFCHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            72390..72797
                     /locus_tag="QIE41_000175"
     CDS             72390..72797
                     /locus_tag="QIE41_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDS3529109.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMNT"
     gene            complement(72918..73910)
                     /gene="rpoS"
                     /locus_tag="QIE41_000176"
     CDS             complement(72918..73910)
                     /gene="rpoS"
                     /locus_tag="QIE41_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDS3529110.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(73973..75112)
                     /gene="nlpD"
                     /locus_tag="QIE41_000177"
     CDS             complement(73973..75112)
                     /gene="nlpD"
                     /locus_tag="QIE41_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDS3529111.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(75252..75878)
                     /gene="pcm"
                     /locus_tag="QIE41_000178"
     CDS             complement(75252..75878)
                     /gene="pcm"
                     /locus_tag="QIE41_000178"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDS3529112.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(75872..76633)
                     /gene="surE"
                     /locus_tag="QIE41_000179"
     CDS             complement(75872..76633)
                     /gene="surE"
                     /locus_tag="QIE41_000179"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDS3529113.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTSENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(76614..77663)
                     /gene="truD"
                     /locus_tag="QIE41_000180"
     CDS             complement(76614..77663)
                     /gene="truD"
                     /locus_tag="QIE41_000180"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDS3529114.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(77660..78139)
                     /gene="ispF"
                     /locus_tag="QIE41_000181"
     CDS             complement(77660..78139)
                     /gene="ispF"
                     /locus_tag="QIE41_000181"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDS3529115.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(78139..78849)
                     /gene="ispD"
                     /locus_tag="QIE41_000182"
     CDS             complement(78139..78849)
                     /gene="ispD"
                     /locus_tag="QIE41_000182"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDS3529116.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKHVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(78868..79179)
                     /gene="ftsB"
                     /locus_tag="QIE41_000183"
     CDS             complement(78868..79179)
                     /gene="ftsB"
                     /locus_tag="QIE41_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDS3529117.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(79373..79696)
                     /locus_tag="QIE41_000184"
     CDS             complement(79373..79696)
                     /locus_tag="QIE41_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDS3529118.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVIGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(79746..80351)
                     /gene="cysC"
                     /locus_tag="QIE41_000185"
     CDS             complement(79746..80351)
                     /gene="cysC"
                     /locus_tag="QIE41_000185"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDS3529119.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(80351..81778)
                     /gene="cysN"
                     /locus_tag="QIE41_000186"
     CDS             complement(80351..81778)
                     /gene="cysN"
                     /locus_tag="QIE41_000186"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDS3529120.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVGYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADESLPAV
                     QSASVDVVWMAEQPLSPGQRYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATTAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(81780..82688)
                     /gene="cysD"
                     /locus_tag="QIE41_000187"
     CDS             complement(81780..82688)
                     /gene="cysD"
                     /locus_tag="QIE41_000187"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDS3529121.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            82940..83977
                     /gene="iap"
                     /locus_tag="QIE41_000188"
     CDS             82940..83977
                     /gene="iap"
                     /locus_tag="QIE41_000188"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDS3529122.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGTSWHDVRLDNQQHIDKALPGRIERRCRDVIRIM
                     LPLVKELAKAS"
     repeat_region   84059..84819
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=84059..84087
                     /rpt_unit_seq="cggtttatccccgctgacgcggggaacac"
     gene            complement(84916..85209)
                     /gene="cas2"
                     /locus_tag="QIE41_000189"
     CDS             complement(84916..85209)
                     /gene="cas2"
                     /locus_tag="QIE41_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDS3529123.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGVRLVSFLPVDNQ"
     gene            complement(85206..86129)
                     /gene="cas1e"
                     /locus_tag="QIE41_000190"
     CDS             complement(85206..86129)
                     /gene="cas1e"
                     /locus_tag="QIE41_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDS3529124.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(86126..86776)
                     /gene="cas6e"
                     /locus_tag="QIE41_000191"
     CDS             complement(86126..86776)
                     /gene="cas6e"
                     /locus_tag="QIE41_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDS3529125.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(86758..87504)
                     /gene="cas5e"
                     /locus_tag="QIE41_000192"
     CDS             complement(86758..87504)
                     /gene="cas5e"
                     /locus_tag="QIE41_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDS3529126.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNSHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYRLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(87515..88570)
                     /gene="cas7e"
                     /locus_tag="QIE41_000193"
     CDS             complement(87515..88570)
                     /gene="cas7e"
                     /locus_tag="QIE41_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDS3529127.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(88585..89121)
                     /gene="casB"
                     /locus_tag="QIE41_000194"
     CDS             complement(88585..89121)
                     /gene="casB"
                     /locus_tag="QIE41_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDS3529128.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(89118..90680)
                     /gene="casA"
                     /locus_tag="QIE41_000195"
     CDS             complement(89118..90680)
                     /gene="casA"
                     /locus_tag="QIE41_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDS3529129.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRRWDDIWEDGLEAEKLREALLSLDHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGVQTTKFNKDHFIKRGVTEHLCPQCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPKKFDD
                     LIFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICDEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(90778..93435)
                     /locus_tag="QIE41_000196"
     CDS             complement(90778..93435)
                     /locus_tag="QIE41_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDS3529130.1"
                     /translation="MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKSNCF
                     GLTDYFRQLGFADTEQAAQFFAWLLCWHDTGKFARSFQQLYLHPQLKVPEGARKNYEK
                     ISHSTLGYWLWHHYLSEYEELLPSSSLSPRKLKRVMEMWMPMTTGHHGRPPDRIDELD
                     NFLPEDKAAARDFLLEIKVLFPLIEIPAFWDDDEGIELLKQLSWYISATVVLADWTGS
                     STRFFPRVAQAMDIKDYWQKALVQAQNALTVFPPKAQTAPFTGINTLFPFIEHPTPLQ
                     QKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQAFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQQEAPFLEKDDYPWL
                     THVTKSDVHSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IKVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKSCSLQRSSQVIVCTQ
                     VIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILA
                     PVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGE
                     DVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESV
                     SLWLSTCIAGVVKPYANGAHAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIEQRQ
                     EPEMANVILVTDDESCGYSAMEGLTGKVG"
BASE COUNT        22098 a        25275 c        23973 g        22115 t
ORIGIN      
        1 gccaaataaa ccattcaaat aacgttcaag cgcaacacgc gaactaaagc catgcgggat
       61 accgtaatgc tcatgacgtt cacccgctaa atagagcgga aattgctggt aatgatcgca
      121 agttttaata tcggaagcgg gttcagccag tgttgaacta cgttctttta atgttggatg
      181 aatcaccagg gcggtacgtc ccattcgcgc ttcgcgatta acatagacat aattttcccc
      241 acggcggtaa ccataagctt tctgggttac cacatcgaca gtaaaaccga ctttttcaag
      301 tacgcgcgcc acctcgtctg gtcgtaaata catatttgat cctcgttatt atcaaccgcg
      361 ggcctacctt acctgattgc gcattcacaa cgctttcaga aaagtccata aagccgcgat
      421 gaacctcagt taagaaaatt atggtctaca ctgaaaatta catcgaattc taatggggga
      481 tcatatgttt aaccgaccga accgcaacga cgttgatgat ggcgtgcagg atatccagaa
      541 tgatgtcaat caattagctg acagcctcga atctgtattg aagtcctggg gcagcgacgc
      601 caaaggggaa gccgaagccg cacgcagtaa agcgcaggct ctactgaaag aaaccagggc
      661 gcgaatgcat ggtcgtactc gcgtccagca agccgcgcgt gatgccgttg gctgcgcgga
      721 ttcttttgtt cgtgaaagac cctggtgtag cgtgggtaca gcagctgcgg taggtatttt
      781 tatcggcgca ctgttaagca tgcgcaaatc gtagtgcaat aatgataata aatacgcgtc
      841 tttgaccccg aagcctggct tcggggtttt ttagcttaag gctttacgta attgttgtgc
      901 tacctgtcgc gccatacctt cgttaacgat attagtaaac gagagcagta atccaccttc
      961 cccacttgaa cggatacgcc agtcgctaag tgcctgcacc gcaaggcctg cagcattggc
     1021 tttacgcgca tgggcaatat catcgcctat cattctgatc accatctgga taccaccttt
     1081 ctgcggcaca acctgaaatc cttgctgcgt caacgcttgc tcaatccatt gccgacgttg
     1141 ggcataatgc tgacgcattt tcttcagatg ccgccagaaa tgcccctcgc gaagaaagtc
     1201 tgccagtgtg ttctgccata gaacaggtac cgcacatgga gccagtgacg cctggtggcg
     1261 gaattgtgca atttgcttca ctggcaacac cagccacgca cagcgcaatg caggaaatag
     1321 cgctttgctg aatgttccgg cataaattac ccgctgcggt gcgtcgagac tttttaacgc
     1381 cggtaacggc ttaccgtgat agcgaaactc actgtcataa tcatcttcaa taatccatgc
     1441 ctgactacga tctgcccatt ccagtatctg atgcctgcgc gctaaagaga gcgccacacc
     1501 cagcggactt tggtgtgctg gtgttatcag ggcaaaacgc gcatcaggat aattttgtat
     1561 tccgcttgtg atatccagtc cattgtcatc aaccggcaca ggcaaaattt ccacatcatg
     1621 gcgagtgaca atcgggcgaa tcagtggaaa gccgggatct tctatccaca tcccgtttcc
     1681 cggtttcgcc agcgcgtgca gaattaaagc tattgaggcc gcataaccgt gagtgataaa
     1741 gacctgctct ggctgacaat caatcccacg tgaaacgcgt aagtaatcaa ctattgcctc
     1801 gcgcaacgcc acctcaccgc agacatcgcc taacgccaaa tcaaagcgcg tctgggtacg
     1861 aaggcgacgc cccatcaccc gcgcccacag ctctcgcgga aagagatcga gtgcgggtaa
     1921 tcccatttga aatggcaacg gttgctgact ttcaccggca aaagctacaa cttgtacagt
     1981 agattgttgc ggatatatcc gctcactgac aaatgtgcca gcctgtcccc gcctgatcag
     2041 ccatccttgc gccaccagct cagcatacgc attttcgaca gtggatcgag acacccccag
     2101 ctcctgcgac caggtacgac ttgaaggcag ccttgcgccg ggtttcagtt ccccttgctc
     2161 aatagccgtt tttaactgac gggcgatatc ctgatagcgc ggcatatggt ctgctttttt
     2221 aggtaattta tggctctctt tagcagtcca ttatagtgct acattgcggg cgttaataag
     2281 aggagaaact gtatgactac attacgtcag ccttactacg aacttagccc ggcagtgtat
     2341 aacgcactag tgcaggccaa aacggcactg gaaaatagca cgctggatac cacgttgatg
     2401 gagttagttt atttgcgcgt ctcgcaaatc aacggctgcg cattttgtct ggagatgcac
     2461 agcaaagcat tgcgcaaatc cggcgtgcca cagcacaaac tggacgccct ggcaggttgg
     2521 cgcgtaagcc atcattttga tgaacgcgag cgggcggcgc tggcatgggc ggaatcggta
     2581 accgaaattg ccagaaccca tgcggaagac gaggtttatc agcctttgct tgagcatttc
     2641 agcgcagcag agatcagcga cttaacgttt gccatcgggc tgatgaattg ttttaaccgt
     2701 ctggccgttt ccatgcggat gtaacttctt gatgccgggt attcgcccgg caaaaaaaca
     2761 caaaatcccc taccccgttg agccattcat cggtggcgta atcaatctaa aattcccata
     2821 tcttgtatgg ttgaatcttt tttcaactac atctagtatc tctgtatcaa cagagagaca
     2881 acccgacgcg tatcatcgcg ccgtatcttc attttaaacg gaaatacgaa taatgcgcat
     2941 tactatttac actcgtaacg attgcgttca gtgccacgcc accaaacggg cgatggaaaa
     3001 ccggggcttt gactttgaaa tgattaatgt cgatcgcgtt cctgaagcgg cagaagcgct
     3061 gcgtgctcag ggctttcgtc agttgccggt tgtgattgca ggcgatctta gctggtctgg
     3121 tttccgcccg gacatgatca atcgtctgca tccagcgcca cacgctgcca gtgcatgagc
     3181 cagctcgtct acttttccag cagctccgaa aatacgcagc gttttatcga acgtttaagg
     3241 ctgcccgcgg tgcgcatccc gctcaatgag cgggaacgga ttcaggtaga cgagccttac
     3301 atcctgatcg tgccctctta cggcggcggc ggtacggctg gcgcggtgcc acgacaggtc
     3361 attcgctttt taaacgacga gcataaccgg gcgttgctgc gcggcgttat tgcttcgggt
     3421 aatcgcaact ttggtgaggc gtatggccgc gccggagatg tgattgctca gaaatgcggc
     3481 gtgccgtggc tgtaccgttt tgaactcatg ggtacgcaaa gcgatatcga aaacgttcgt
     3541 aaaggagtaa ccgaattttg gcaacgacac ccgcagaacg cctgacgcag gaaacgatgg
     3601 attaccacgc gctgaatgcg atgcttaacc tttacgatag cgcaggtcgc attcagttcg
     3661 ataaagatcg ccaggcagta gacgccttta taacgacgca tgtgcgtccg aacagtgtgg
     3721 ccttcagtag ccagcagcag cgcctgaact ggctggttaa cgaaggttac tatgatgaaa
     3781 gcgtccttaa tcgctactct cgcgattttg tcattacgct gtttgcccac gcgcacgcca
     3841 gcggttttcg tttccagaca ttcctcggag catggaagtt ttacaccagc tatacgttga
     3901 agacattcga cggtaagcgt tatctggaag attttgccga tcgagtcacg atggtggcgc
     3961 tgacgctggc acaaggcgat gaaacactgg cgacgcaact gaccgatgag atgctgtcag
     4021 gacgctttca gccagccacg ccgacattcc tcaactgcgg taagcagcag cgcggcgaac
     4081 tggtttcctg ttttctgctg cgtattgaag acaatatgga gtcgattggc cgggcggtaa
     4141 attccgcact gcaactgtcg aaacgcggcg gcggcgtagc atttttgctg tcgaatctgc
     4201 gagaagcggg cgcgccaatt aaacgtattg aaaatcaatc atctggcgtg attccggtga
     4261 tgaaaatgct ggaagacgca ttttcctatg ccaaccagct cggcgcgcgt cagggggcag
     4321 gtgcagtcta tttacatgct catcatcccg atattctgcg ttttctcgat acaaaacggg
     4381 aaaatgccga cgaaaaaatc cgcattaaaa cactgtcgct gggcgtggtg atcccggata
     4441 tcactttcca tctggcaaaa gagaatgcgc agatggcgct gttttcgcct tatgacgtag
     4501 agcgagttta tggcaagccg tttgccgatg tcgccattag cgaacactat gacgaactgg
     4561 ttgccgatga acgcattcgc aaaaaatacc tcaacgcccg tgatttcttc cagcgactgg
     4621 cagaaatcca gtttgagtcc ggctatccct acatcatgta tgaagacacg gttaaccgtg
     4681 ctaaccctat cgccgggcgc ataaatatga gcaatctctg ctcagaaatt ttgcaggtta
     4741 acagcgcctc agagtatgac gagaatctcg actatgcccg cacaggccat gatatttcct
     4801 gcaatttagg ttcgttgaat attgcccaca ccatggattc ccccgacttt gcccgcacgg
     4861 tagagactgc cgtgcgcggt ttaacggcgg tgtcagatat gagtcatatc cgcagcgtgc
     4921 cgtccatcga agccggaaat gccgcctcac acgccatcgg actggggcag atgaatttac
     4981 acggctatct ggcgcgagaa ggcatcgctt atgggtcgcc ggaagcactg gatttcacca
     5041 atctctattt ctataccatc acctggcacg cactgcgtac ctcgatgttg ctggcgcgcg
     5101 aacgcggtga aaccttcgcc gggttcaaac agtcacgcta tgccagtggt gaatatttta
     5161 gccaatatct gcaagggaac tggcagccga aaacggcgaa agttggcgaa ctgtttaccc
     5221 gtagcggtat tacgttacct acccgtgaga tgtgggcgca gctgcgcgac gacgtgatgc
     5281 gctacggcat ttacaaccag aatcttcagg cggtgccgcc aaccggttct atctcttata
     5341 tcaaccatgc tacgtcgagt attcatccga ttgtggcgaa agtagagata cgcaaagagg
     5401 gcaaaacagg acgcgtttac taccctgccc cgtttatgac taacgagaat ctggcgctgt
     5461 atcaggacgc ttacgaaatt ggcgcagaaa agatcatcga cacttacgcg gaagcgactc
     5521 gccatgtcga tcaggggctg tcgctgacgc tgtttttccc cgataccgcc accactcgcg
     5581 atatcaacaa agcgcagatt tacgcctggc gcaaaggtat caaaacgctc tattacatcc
     5641 gcctgcgtca gatggcgctg gaaggcactg aaattgaagg ctgcgtctcg tgtgcacttt
     5701 aaggaatatc tatgaaactc tctcgtatca gcgccatcaa ctggaacaag atatctgacg
     5761 ataaagatct ggaggtgtgg aatcgcctga ccagcaattt ctggctacca gaaaaggtgc
     5821 cgctgtcgaa cgatattcct gcatggcaga cattaagtgt cgtagaacaa caactgacga
     5881 tgcgcgtttt tactggcctg acgctgctcg acacgctgca aaatgttatc ggcgcgcctt
     5941 cgctgttgcc cgatgcactc acgcctcatg aagaagcggt attatcgaat atcagcttta
     6001 tggaagcggt tcatgcccgc tcttacagtt cgattttctc gacgctgtgc cagaccaaag
     6061 atgtcgatgc cgcctacgcc tggagtgaag aaaacgcacc gttgcagcga aaagctcaga
     6121 ttattcagca acattatcgc ggggatgatc cgctgaaaaa gaaaatcgcc agtgtgtttc
     6181 ttgaatcttt tttgttctat tccggtttct ggctgccgat gtatttttcc agccgcggaa
     6241 agctaaccaa taccgcagac ctgatccgtc tgattatccg cgatgaagca gtccacggtt
     6301 attacatcgg ctataaatat cagaaaaaca tggaaaagat ctcccaggca caacgtgaag
     6361 agttgaagag tttcgccttc gatttgttgc tggaacttta tgacaacgag ttgcaataca
     6421 ccgatgagct gtacgccgaa accccgtggg ctgacgatgt gaaagcgttt ctctgttaca
     6481 acgccaataa ggcgttgatg aatctgggct acgaaccgtt atttcccgca gaaatggcgg
     6541 aagtgaatcc ggcaatcctc gccgcgcttt cgccgaatgc cgatgaaaat cacgatttct
     6601 tttccggttc aggctcttca tatgtgatgg gaaaagcggt cgaaacagaa gatgaagact
     6661 ggaatttctg agggtgttat ttttcaaaaa tatcactacc cgcagcaggg aaataatttc
     6721 cgccaaatag ctttttatca cgcaaatatt ttgtggtgat ctacactgac actctgttgc
     6781 attattcgcc tgaaaccaca atattcagac gttttttcgc tatctttgac aaaaaatatc
     6841 aacttttctc gatttgctct cagcccttat atcatgggaa attccggcga tttgctcaca
     6901 tcaatattca tgccacattt gccatcaggg gttgcctcag attctcagta tgttagggta
     6961 gaaaaaagtg actatttcca ttgggtaatg tatcgacata aacaaataac aggaatcttt
     7021 ctattgcatg gcaattaaat tagaaattaa aaatctttat aaaatatttg gtgagcatcc
     7081 acagcgagct ttcaaatata tcgaacaagg actttcaaaa gaacaaattc tggaaaaaac
     7141 tgggctatcg cttggcgtaa aagacgccag tctggccatt gaagaaggcg agatatttgt
     7201 catcatggga ttatccggtt cgggtaaatc cacaatggta cgccttctca atcgcctgat
     7261 tgaacccacc cgcgggcaag tgctgattga tggtgtggat attgccaaaa tatccgacgc
     7321 cgaactccgt gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat
     7381 gccgcatatg accgtgctgg acaatactgc gttcggtatg gaattggccg gaattaatgc
     7441 cgaagaacgc cgggaaaaag cccttgatgc actgcgtcag gtcgggctgg aaaattatgc
     7501 ccacagctac ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc
     7561 gttagcgatt aatccggata tattattaat ggacgaagcc ttctcggcgc tcgatccatt
     7621 aattcgcacc gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat
     7681 tgtctttatt tcccacgatc tcgatgaagc tatgcgtatt ggcgaccgaa ttgccattat
     7741 gcaaaatggt gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa
     7801 tgattatgtc cgtaccttct tccgtggcgt cgatattagc caggtgttca gtgcgaaaga
     7861 tattgcccgt cgtacaccga acggcttaat tcgtaaaacc cccggcttcg gcccgcgttc
     7921 ggcactgaaa ttattgcagg atgaagatcg cgaatatggc tacgtcatcg aacgcggtaa
     7981 taagtttgtc ggcgctgtgt ctatcgattc gcttaaagcc gcgttaacgc agcagcaagg
     8041 tcttgatgcg gcgctgattg atgcgccgtt agcggtcgat gcacaaacgc ctcttagcga
     8101 gttgctctct catgtcggac aggccccctg tgcggtgccc gtggtcgacg aggaccaaca
     8161 gtatgtcggc atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa
     8221 taatggctga tcaaaataat ccgtgggata ccacgccagc ggcggacagt gccgcacagt
     8281 ccgcagacgc ctggggtaca ccggcgactg caccgactga cggcggtggt gctgactggc
     8341 tgaccagtac gccagcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa
     8401 cgctgatccc gctcgacagt tgggtcactg aagggatcga ctgggtcgtt acccatttcc
     8461 gtcccgtctt ccagggcgtg cgcgttccgg ttgattacat cctcaacggt ttccagcaat
     8521 tgctgctggg tatgcccgct ccggtggcga ttatcgtttt cgctctcatc gcctggcaaa
     8581 tttccggtgt cggaatgggc gtggcgacgc tggtttcgct gattgccatc ggcgcaatcg
     8641 gtgcctggtc acaggcaatg gttactctgg cgctggtgct aaccgccctg ctgttctgta
     8701 tcgtcatcgg tttgccgttg gggatctggc tggcgagaag tccacgagcg gcgaaaatta
     8761 ttcgtccact gcttgatgcc atgcagacca cgccagcgtt tgtttatctg gtaccaatcg
     8821 tcatgctgtt tggtatcggt aacgtgccgg gcgtagtggt gacgatcatc tttgctctgc
     8881 cgccgattat ccgtctgaca attctgggga ttaaccaggt tccggcggat ctgattgaag
     8941 cctcgcgctc attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg
     9001 cgatgccgac cattatggcg ggcgttaacc agacgctgat gctggccctt tctatggtgg
     9061 tcatcgcctc gatgattgca gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc
     9121 gtctggatat ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc
     9181 tcgaccgcct gacgcaggcc gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt
     9241 acaccactgg ccctgttggt ttgctgaccc gcccattcat taagtaactc tgcacttgcc
     9301 cggtgacgcc gggcattatc accctgccaa aaaaaggaat aacaatgcga catagcgtac
     9361 tttttgcgac agcgtttgcc acgcttatct ctacacaaac ttttgctgcc gatctgccgg
     9421 gcaaaggcat tactgttaat ccagttcaga gcaccatcac tgaagaaacc ttccagacgc
     9481 tgctggtcag tcgtgcactg gagaaattag gttataccgt caataaaccc agcgaagtgg
     9541 attacaacgt tggctacacc tcgcttgctt ccggcgatgc aaccttcacc gccgtgaact
     9601 ggacgccgct gcatgacaac atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg
     9661 aaggagtatt tgttaacggc gcggcacagg gttacctgat cgataagaaa actgccgacc
     9721 agtacaaaat caccaacatc gcgcaactga aagatccgaa gatcgccaaa ctgttcgata
     9781 ccaacggcga cggtaaagcg gatttgactg gctgtaaccc tggctggggc tgcgaaggtg
     9841 cgatcaacca ccagcttgcc gcgtatggac tgaccaatac cgtgacgcat aatcagggga
     9901 actacgcagc gatgatggct gacaccatca gtcgttacaa agagggtaag ccggtgtttt
     9961 actacacctg gacgccgtac tgggtgagta acgaactgaa accggggaaa gatgtggtct
    10021 ggttgcaggt gccgttctcc gcactgccgg gcgataaaaa tgccgatacc aaactgccga
    10081 atggcgcgaa ttacggcttc ccggtcagta ccatgcatat cgttgccaac aaagcctggg
    10141 ccgagaaaaa cccggcagca gcgaaactgt ttgccattat gcagttacca gtggcagata
    10201 ttaacgccca gaacgccatt atgcatgacg gcaaagcctc agaaggcgat attcagggcc
    10261 acgttgatgg ctggatcaaa gcccaccagc agcagttcga tggctgggtg aatgaggcgc
    10321 tggcagcgca gaagtaaata ttgataaccg cgcagataat gctcatgtga gtaaaacaat
    10381 tgcagcgatg gttttaatcc ggctgaaaat cgatgagacg ttttctgaag gccggggcga
    10441 cgcgcaggga tgcgcggcga gggcgcgtct tacagggatg ttacctcgcg cccgtacccg
    10501 atagccgtaa gaaataagtc gaatgtaccg cgtagcggcg attttgtgcc aaaagtttgg
    10561 gtcgccaggg tggtggcgac tgagccaccc tggcacgttc accagcaaaa tagtgacaaa
    10621 gtagctataa tctggaagtg aacgtaacat ttgccttagt ggcatatttc accttcaaat
    10681 accctctccc cattacccgc acattttaac aatctatctt tcgccccata ttcatcaaca
    10741 tccgctatta ttgatttcca gcttaatcat cacctgatga acaaaaataa tgactaaacc
    10801 taatcatgag cttagcccgg cactgatcgt actgatgtct atcgccaccg gtctggcggt
    10861 cgccagcaac tattacgccc agccattgct cgacaccatt gcgcgtaact tttccctttc
    10921 cgccagttcg gcgggcttta ttgttaccgc cgcgcagttg ggctatgccg caggtctgct
    10981 gtttcttgtt cccctcggtg atatgtttga acgccgccgc ctgattgtct cgatgacctt
    11041 actggcagcg ggcggtatgt taatcactgc cagcagtcag tcactggcga tgatgatcct
    11101 cggtacggca ttaaccggtt tattctcagt cgtggcacaa attctggttc cgctggcagc
    11161 gacgctggct tcaccggata aacgcggcaa agtggtcggc accattatga gcgggctgct
    11221 gttggggatc ttgctggcac gaaccgttgc cgggttgctg gcaaacctcg gcggctggcg
    11281 caccgtcttt tgggttgcct cggtgttaat ggcactgatg gcgctggcat tatggcgtgg
    11341 tctgccacaa atgaaatcag aaacccacct caactaccca cagttgcttg gttccgtttt
    11401 cagcatgttt atcagcgata aaattctgcg cacccgcgcg ttgctgggct gcctgacctt
    11461 tgccaacttc agcattctct ggacctcaat ggcctttttg cttgccgctc caccttttaa
    11521 ctacagcgat ggtgtaattg gtctgtttgg acttgcggga gctgccggag cgttgggtgc
    11581 tcgtccggcg ggcggttttg ccgataaggg taaatcgcac cacaccacta ctttcggtct
    11641 gctgctgcta ttactttcat ggctggcgat ctggtttggc cacacttccg tactggcctt
    11701 gattatcggc atcctggtgc tggatctcac cgtgcagggc gtgcatatca ctaaccagac
    11761 ggtaatttat cgaatacacc ctgatgcgcg taatcgcctg accgcaggtt acatgaccag
    11821 ctactttatt ggcggtgccg ccggttcgct aatttcagcc tcagcctggc aacatggcgg
    11881 ttgggctggc gtttgtctgg ctggcgcgac gattgccctg gttaacttac tggtctggtg
    11941 gcgaggtttt catcgtcagg aagctgcaaa ttaagcaatc agcaaccgtt ttcgtggctt
    12001 tacacttata agggtgttaa gaagcccatc agtctgataa ggttaagata ttcattcagt
    12061 ctatttataa tattaacaat cgttaagcgt acactctatg gaaagcccta ctccacagcc
    12121 tgctcctggt tcggcgacct tcatggaagg atgcaaagac agtttaccga ttgttattag
    12181 ttatattccg gtggcctttg cgttcggtct gaatgcgacc cgtctgggat tctctcctct
    12241 cgaaagcgtt tttttctcct gcatcattta tgcaggcgcg agccagttcg tcattaccgc
    12301 gatgctggca gccgggagta gtttgtgggt tgctgcactg accgtcatgg caatggatgt
    12361 tcgccatgtt ttgtatggcc cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc
    12421 gaaaaccgcc ctgtgggcat ttggcctgac ggatgaggtt tttgccgccg caaccgcaaa
    12481 actggtacgc aataatcgcc gctggagcga gaactggatg atcggcattg ccttcagttc
    12541 atggtcatcg tgggtatttg gtacggtaat aggggcattc tccggcagcg gcttgctgca
    12601 aggatatccc gccgttgaag cagcattagg ttttatgctt ccggcactct ttatgagttt
    12661 cctgctcgcc tctttccagc gcaaacaatc tctttgcgtt accgcagcgt tagttggtgc
    12721 ccttgcaggc gtaacgctat tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg
    12781 ctgcctcact gcgttaatcc aggcattctg gcaaggagcg cccgatgagc tatgaggttc
    12841 tgctgcttgg gttactggtt ggcgcggcga attattgctt ccgctatttg ccgctgcgcc
    12901 tgcgtgtggg taatgcccgc ccaaccaaac gtggcgcgat aggcatattg ctcgacacca
    12961 ttggcatcgc ctcgatatgc gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg
    13021 atacacgccg tttcgtgccc acgctggtcg gcttcgcggt actgggtgcc agtttctata
    13081 aaacacgcag cattatcatc ccaacactgc ttagtgcgct ggcctacggg ctcgcctgga
    13141 aagtgatggc gattatataa cccacaagaa tcatttttct aaaacaatac atttacttta
    13201 tttgtcactg tcgttactat atcggctgaa attaatgagg tcatacccaa atggatagtt
    13261 cgtttacgcc cattgaacaa atgctaaaat ttcgcgccag ccgccacgaa gattttcctt
    13321 atcaggagat ccttctgacc cgtctttgca tgcacatgca aagcaagctg ctggagaacc
    13381 gcaataaaat gctgaaggct caggggatta acgagacgtt gtttatggcg ttgattacgc
    13441 tggagtctca ggaaaaccac agtattcagc cttctgaatt gagttgtgct cttggatcat
    13501 cccgtaccaa cgcgacgcgt attgccgatg aactggaaaa acgcggttgg attgaacgtc
    13561 gtgaaagcga taacgatcgc cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt
    13621 ttttgcgcga ggttttacca ccgcagcata actgcctgca tcaactctgg tccgcgctca
    13681 gcacaacaga aaaagatcag ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc
    13741 agatggaaca agacggtgtg gttctcgaag cgatgagcta acgcgtcatc tcgctcaaaa
    13801 atccagattt ataaaagaaa aaatgactgg ccagcatcgc aacatgctgg cctttttggc
    13861 aagcaggtcg gctcagccga tgagttaaga agatcgtgga gaacaatatg agcgcaaatg
    13921 cggagactca aaccccgcag caaccggtaa agaagagcgg caaacgtaag cgtctgctcc
    13981 tccttctcac cttgctcttt ataattattg ccgtagcgat agggatttat tggtttttgg
    14041 tactgcgtca tttcgaagaa accgatgacg catacgtggc agggaatcag attcaaatta
    14101 tgtctcaggt gtctggcagc gtgacgaaag tctgggccga taacaccgac tttgtaaaag
    14161 aaggcgatgt gctggtcacc ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca
    14221 aaactgcact ggcttccagc gttcgccaaa cccaccagct gatgattaac agcaaacagt
    14281 tgcaggcgaa tattgaggtg caaaaaatcg cactcgcgaa agcacaaagc gactacaacc
    14341 gccgtgtgcc gctgggcaat gccaacctga ttggtcgcga agagctgcaa cacgcccgcg
    14401 acgccgttac cagtgcccag gcacaactgg acgtcgcgat tcaacaatac aatgccaatc
    14461 aggcgatgat tctggggact aaactggaag atcagcctgc cgtgcaacag gctgccaccg
    14521 aagtacgtaa cgcctggctg gcgctggagc gtactcgtat tgtcagtccg atgaccggtt
    14581 atgtctcccg ccgcgcggta cagcctgggg cacaaattag cccaacgacg ccgctgatgg
    14641 cggtcgttcc agccaccaat atgtgggtgg atgccaactt taaagagacg cagattgcca
    14701 atatgcgcat cggtcagccg gtcaccatca ccaccgatat ttacggcgat gatgtgaaat
    14761 acaccggtaa agtggttggt ctggatatgg ggacaggtag cgcgttctca ctgcttccgg
    14821 cgcaaaatgc gactggtaac tggatcaaag tcgttcagcg tctgcctgtt cgtattgaac
    14881 tggaccagaa acagctcgag caatacccgc tgcgtatcgg tttgtccacg ctggtgagcg
    14941 tcaataccac taaccgtgac ggtcaggtac tggcaaataa agtacgttcc acaccggtag
    15001 cggtaagcac cgcgcgtgaa atcagcctgg cacctgtcaa taaactgatc gacgatatcg
    15061 taaaagctaa cgctggctaa tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa
    15121 ggcgcgcaac tggtcattat gacgattgcg ctgtcactgg cgacattcat gcaggtgctg
    15181 gactccacca ttgctaacgt ggcgatcccc actatcgccg ggaatctggg ctcatcgctc
    15241 agccagggaa cgtgggtaat cacttctttc ggggtggcga atgccatctc gatcccgctt
    15301 accggctggc tggcaaagcg cgtcggggaa gtgaaactgt tcctctggtc caccatcgcc
    15361 tttgctattg cgtcgtgggc gtgtggtgtc tccagcagcc tgaatatgct gattttcttc
    15421 cgcgtgattc aggggattgt cgccgggccg ttaattccgc tttcgcaaag tctattgctg
    15481 aataactacc cgccagccaa acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt
    15541 gttgcgccaa tttgcggccc gatcctcggc gggtatatca gtgacaatta ccactggggc
    15601 tggatattct tcatcaacgt accgattggc gtggcggtgg tgttgatgac actgcaaacc
    15661 ctgcgcggac gtgaaacccg caccgaacgg cggcggattg atgccgtggg gctggcactg
    15721 ctggttattg gtatcggtag cctgcagatt atgctcgacc gcggtaaaga gctggactgg
    15781 ttttcatcac aggaaattat catccttacc gtggtggcag tggtggctat ctgcttcctg
    15841 attgtctggg agctgaccga cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc
    15901 aacttcacca tcggctgttt gtgtatcagc ctcgcgtata tgctctactt cggcgctatt
    15961 gttctgctgc cgcagttgtt gcaggaggtc tacggttaca cggcaacgtg ggcaggtctg
    16021 gcctctgcgc cggtaggaat tattccggtg atcctgtcgc cgattatcgg ccgcttcgcg
    16081 cataaactgg atatgcggcg gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc
    16141 tactggcgtg cctatacctt tgaaccgggt atggattttg gcgcgtcggc ctggccgcag
    16201 tttatccagg ggtttgcggt ggcctgcttc tttatgccgc tgaccaccat tacgctgtct
    16261 ggtttgccac cggaacgact ggcggcggca tcgagcctct ccaactttac gcgtacgctg
    16321 gcggggtcta tcggcacgtc gataaccaca accatgtgga ccaaccgcga gtcgatgcac
    16381 catgcgcagt taaccgagtc ggtaaacccg tttaacccaa acgcccaggc gatgtacagt
    16441 caactggaag gactgggaat gacgcaacag caggcatcag gctggattgc ccagcagatc
    16501 accaatcagg ggctgattat ttccgccaat gagatcttct ggatgtcagc cgggatattc
    16561 ctcgtcttgc tggggctggt gtggtttgct aaaccgccat ttggcgcagg tggcggcgga
    16621 ggtggtgcgc actaagtaca actaagccag ttcatttgaa ctggcttttt tcaattaatt
    16681 gtgaagatag tttactgact agatgtgcag ttcctgcaac ttctctttcg gcagcgccag
    16741 ttcttcgttg ctgttgatgc gcacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc
    16801 ttcctgcaac gagtgcatct ggtaagtgcc acactggtag acgttcagtt ccgggatctg
    16861 attctgatcc tgcactttca gcacgtcttc cattgccgct ttccaggcat cagcaacacg
    16921 ctgctcatct ggcgtaccaa tcagactcat ataaaaaccg gtgcggcagc ccattggcga
    16981 gatatcgata atctctacac cattaccgtt aagatggtta cgcataaaac cagcaaacag
    17041 gtgctccagg gtatggatcc ctctttctgg catcacttct ttgttcggca cgcagaagcg
    17101 cagatcgaac acggtgattg cgtcgccatg cggggtgttc attgttttcg ccacccgaac
    17161 tgcaggcgct tccatccggg tatgatcgac tgtgaagcta tctaacaacg gcatttagcc
    17221 acctccggta atttttttaa aaattttctg aactctttct tcccaggcga gtctgagtat
    17281 atgaaagacg cgcatttgtt atcatcatcc ctgaattcag agatgaaatt ttggccactc
    17341 acgagtggcc tttttctttt ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg
    17401 gtatcagcgg cttccatttc ctgctgacgg cgttcagacg cctcgcgctc ggctacaaaa
    17461 tcctcttcgc gcagaatttc caacggctct tcacgcagca gattacggta cgcttcagca
    17521 aacgctttgc ctgttccgcc aataccagta tcaatcatag accttaagat acgggcagag
    17581 aaagtcagat cgggattatc gaagcaggcg accagttcat cacacacttt ctgatacgct
    17641 tcgccgccgt taatgctatc cagcgtttgc gcaacgcgtt tcagatcgcg gaacagatct
    17701 ttacccacct gcggtaacgg gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc
    17761 ggtttgcgac cttcgaggat cacccggttc cagttaacgc gtgtacaggc aagttcttta
    17821 ctgctcattt ccggtgcatc agccagcgca caccagacca taaacaggtc gaggaatcgc
    17881 acctgctgtt catctacacc aatcggcgag aatgggttga tgtccagcga acgcacttca
    17941 atatattcaa tgccgccacg taacagcgca tcagaaggcg actcgccgct gcgggtaacg
    18001 cgttttggac gaatcggcgc gtacagttcg ttttcaatct gcagcacgtt gctgttgatt
    18061 tgcagcctct taccatcttt ctcaatgcca atcttggcgt actcttccga tggcgttttg
    18121 attgcctgtt taaggcccgc tacgtactcg taaagatcgt tgaaggtaat acctagattg
    18181 ctttgcgatt tattggtata gccgagatca ctcaaacgaa gagaggtcgc atacggcagg
    18241 taatacatac cgcactcggt tttctcaaac ggcagcgatg ttggttttcc ttgcaggaaa
    18301 gaagaacaaa tcgccggaga cgcgccaaac agataaggaa tgacccaacc gaaacgatag
    18361 taattgcgga taacgcggaa atagcccgca gaaattttct ctttggcatc agcgcccgcg
    18421 atatcaccac acttcgcttg ccagaatgcc attggcaaag agaaattgta gtgcacgccg
    18481 gaaatggttt gcatcagcgc gccgtagcga tttttcagcc cttcacggta cagcgtttta
    18541 aagcgtccgg tgttagaagt gccgtactgt gccagttcga tgtcctgacc ttctgcgatg
    18601 tagcatggca tacttaacgg ccacatccgc tcatcgccca tattgcgcgc cgtataacga
    18661 tgcagatcgc gcataaaggt cagcatatgt tcaatatcac catccactgg tgtaatgaat
    18721 tccagcaatg cttccgcaaa atcggtagta atccatttgt gcgtcagtgc ggaacctaat
    18781 gcttcaggat gacctgttgt tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc
    18841 tccagcccac gctgtatccc ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc
    18901 tgtgatacgt ccgggatcaa attgacctcc cgcctgtcaa aatcgtttta attagcataa
    18961 ctgtaatggt gaccatatgt gcaggcctac aattagtgcc accacatcat gccctgaacg
    19021 gtcgctgctg caactgcaac atagcgtaac gctttaccaa ggcacaaaaa aaagattacc
    19081 ggcccccagg agatgcgcat ccatcccgct aacagacaca gtaaatcgcc aaccacgggc
    19141 atccagctta ataatagcgt gactgcacca tagcgtttca gccagccggt agctttctct
    19201 tgccagcgcg atgttttacg caatggaaag aaacgcccaa ggataacgtt agttaaccct
    19261 ccaaggctat tacccattgt tgctgttaaa actaaaaccc agggatgact gatcccggaa
    19321 agcaacattg ccaccagcac gacttcggag ttgccgggta atagtgtagc gctgagaaaa
    19381 ctactggcaa acaacgagaa aagcgataac gcttcactca cagcaagcga acatccacag
    19441 cgtccatgcc tgctgcacgg gccgcctgaa taccgaaatc ggcatcttca aagaccacac
    19501 actgtgtcgg ttgcacgccc atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt
    19561 tatggtgttt gacgtgatca gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt
    19621 gcgccagcaa tgcctcagcg atggcgcttt cactccccgt tcctacagcc attgggcgac
    19681 gaccatgcca gcttttcacc acttcaacaa gaggaagcgg ctcgacacta tccagcagca
    19741 tacttcttac tgcttctgtt ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat
    19801 tcagctcaat aatcgcctga gcaatacgcc aggtgggcga gccattaagc gcaatcatcg
    19861 cctgaacatc gtactgaaga ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg
    19921 taggctcggt atccaggatg gtgccatcca tatcaaaaat taaacctgca taacgctcgt
    19981 acatggtctt ctcgcaagtc gaaaaatatg acgctacttt agcgtaattg cttgattttg
    20041 tcgctgatgg cgaaggagaa gaaaattcag gaaatggata aagtagttat ttagaaatga
    20101 gatatttttg aaggaaattt tttggagaag atggtgcatc cgggaggatt cgaacctccg
    20161 accgctcggt tcgtagccga gtactctatc cagctgagct acggatgcat cgggaaaact
    20221 tattttactg ctgatattga taccgctact aaagccatat caaataagaa aatccatctt
    20281 aaccatcatc actgatgatt acggagaata tggtgcatcc gggaggattc gaacctccga
    20341 ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc gggaaattta
    20401 ttttactgct gatattgata ccgctgctaa agccatatca agtaagagat ggtgcatccg
    20461 ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca gctgagctac
    20521 ggatgcatcg ggaaacttac tttactgcag attttttgat accgctacta aagccgtatc
    20581 aagtaagaga tggtgcatcc gggaggatta ctcgccttgc ggctcgccct tcgggccgtt
    20641 gctaaagcaa cgttatcctc cctggtgcct gcggttatta ccgccacctc ttccatacct
    20701 tcatcgctga aagtacgtag aatatggtgc atccgggagg attcgaacct ccgaccgctc
    20761 ggttcgtagc cgagtactct atccagctga gctacggatg caaatggcgg tgaggcgggg
    20821 attcgaaccc cggatgcagc ttttgaccgc atactccctt agcaggggag cgccttcagc
    20881 ctctcggcca cctcaccaca cgcctcttac gagtgcttcg aagaacttgt ttatcgctca
    20941 tcgtcgctgc gtggcgcaca tattactttc tgagacttat aagtcaaaca atttttccca
    21001 cacttttatc gtttgcacac ttcacgctca attagtctgt aaaaacgtca aaaagagtgt
    21061 tttatcaaca gaaaaacgga ggtctgtcag atagcagtaa tgcaaaaaaa tggagactta
    21121 agttgaatga acgggagtaa agcgaaaaga caatggagtg aaggagatat tttgaggggt
    21181 gcgtctcacc gataaagatg agacgcggaa agattagtaa ctggactgct gggatttttc
    21241 agcctggata cgctggtaga tctcttcacg gtgaacagaa acttccttcg gggcatttac
    21301 gccaatacgt acctggttgc cctttacccc taaaactgtc acggtgacct catccccaat
    21361 catgagggtc tcaccaactc gacgagtcag aatcagcatt ctttgctcct tgaaagatta
    21421 aaagagtcgg gtctctctgt atcccggcat tatccatcat ataacgccaa aaagtaagca
    21481 atgacaaaca cattacatgt aagcagtcat ggcattacat tctgttaaac ctaagtttag
    21541 ccgatataca caacttcaac ctgactttat cgttgtcgat agcgttaatg cgaatgccgt
    21601 gaagcgagtc cacggcattg cctgacgctt atattattgc aatttcgcgc tgacccagcc
    21661 tttcacactg gctaacgctg caggtaaggc cgcagcatcc gtaccaccgg cttgcgccat
    21721 gtcaggacgt ccaccaccct tgccgcccac ctgctgagcg accataccaa tcagttcccc
    21781 tgctttcaca cgatctgtga cgtccttaga tacgcctgca atcagagaaa ccttaccttc
    21841 ggctaccgtt gccagcacga taattgtcga ccccagctga ttttttaaat cgtcaaccat
    21901 ggtacgcaac attttcggct caacaccgct aagctcgcta accaacagct taacaccatt
    21961 aacatcaatt gccttactgg aaagatttgc gctctcctgt gcggcagctt gttctttaag
    22021 ctgttgtaac tctttttcca gctgacgcgt acgttccagt actgagcgca ctttatcagc
    22081 cagattattg ctatcgcctt tcagcagatg cgcgacttcg tttaagcgat cgctgtctgc
    22141 atgaacggtg gcgatagcgc cttctccggt taccgcttcg atacgacgaa cgcctgcagc
    22201 agtacccgat tcagagatga tgcggaacag accaatatca ccagtgcggc tggcgtgagt
    22261 accgccacac agctcggtgg agaaatcgcc catgctcagc acgcgtacgc gctcatcata
    22321 cttctcgccg aacagcgcca tcgcaccttt cgctttcgcc gcttcgagat ccatgatgtt
    22381 ggtttcgatc ggcaagttac ggcgaatctg tgcgttcacc aggtcttcga ccgcacgaat
    22441 ctcttctggt ttcatcgctt cgttgtgtga gaagtcgaag cgcagcactt tgtcgttaac
    22501 cagtgaacct ttctgcgata catgagtacc cagaacctgg cgcagcgcag cgtgcatcag
    22561 gtgcgttgcg gagtgattca gacgaatacg ggcgcgacga gcctcatcaa catcagcctg
    22621 caccgcgtcg cccactttca gagaacccgc agcaagttta ccgatgtgac caatcgcctg
    22681 gccgtatttc tgcgtatctt ccaccgcaaa ggagaagtta gcgcctttca gttcgccttt
    22741 atcaccaacc tgaccgccgg attccgcata gaatggcgtt tgatccagca cgaccacagc
    22801 ttcctggcct gcattgatgg catcaaccgc tttaccatca acaaacagcg cggtcacttt
    22861 gccgttcagt tccagatggt catagccttt aaattcagat gcgctgtcaa cacggatcat
    22921 tgcgttgtaa tcggcaccaa agccgctggc ttcgcgcgcg cgacgacgct gctcttccat
    22981 tgctgcttcg aaaccagctt cgtcaacttt gatgttgcgc tcacgacaaa catcagccgt
    23041 caggtcaacc gggaagccat aggtgtcgta cagacggaaa gccgtttcgc catccagcgt
    23101 atcaccagaa agttttgcca gctcttcatc cagcagcgcc agaccgcgct ccagagtacg
    23161 ggcaaactgc tcttcttcag tcttcagcac ctgctcaacc tgcgcctgct ggcgtttcag
    23221 gtcttcaccc gcagagccca ttacgtcgat cagcggacca accagtttgt agaagaaggt
    23281 ttctttcgcg cccagcatat tgccgtgacg cactgcgcga cgaatgatac gacgcagtac
    23341 ataaccacgg ttttcattgg acggcattac gccatccgcg atcaggaacg cacaagaacg
    23401 aatgtggtca gcgattacgc gcagcgattt attgctcaga tcggtcgcgc cagtgacttt
    23461 cgctaccgcc tggatcagcg tgcggaacag gtcgatgtca tagttagagt taacgtgttg
    23521 cagcaccgca gcaatacgct ccagacccat accggtatct acagacggct tcggcagcgg
    23581 ttccatcgtg ccatctgcct ggcggttgaa ctgcatgaag acgatgttcc agatctcaat
    23641 gtagcgatcg ccgtcttctt ccggacttcc cggagggcca ccccaaatgt ggtcgccgtg
    23701 atcgtagaag atttcggtgc acgggccgca cggaccagtg tcacccatct gccagaagtt
    23761 gtcagatgcg tatggcgcac ctttgttatc gccgatgcga ataatacgtt cgcgcgggat
    23821 ccctacttct ttttcccaga tttcgtaggc ttcgtcgtcg ctttcataga cggtaaccca
    23881 cagacgctct ttcggcaggg caaaccattt ttcactggtc agcagttccc atgcaaactg
    23941 aatggcatcg tgtttgaaat agtcgccgaa gctgaagttg cccagcattt cgaagaaggt
    24001 atgatgacgc gcggtgtaac cgacgttttc caggtcgttg tgtttaccac ccgcacgcac
    24061 gcagcgttgg gaagtggtag cgcgggaata attacgcttg tcgagcccaa ggaacacatc
    24121 cttgaactgg ttcatcccgg cgttggtaaa caacaaagtt gggtcgttat ggggtaccag
    24181 ggagctgctg gcaactacct gatgtccctt actatggaaa aagtcgagaa acgcctgacg
    24241 gatctcagcg gtgctcttgc tcataattat cctgaaatca agctaacgaa atatcgccac
    24301 cagctccagc gtgtcttaac cgccgggctg gtaactgaaa agtgggaata agataagttt
    24361 tcttgactgg gaagtaaaat accgtatgcg ttcagtcggc aaaatttcgc caaatatcct
    24421 ggatatcttc catcagatag ccacgataga gcagaaaacg ctggatctta actttttctg
    24481 aaaagacagt tggcaaaggt tcgccatatt ttcgcgtcgc ctgatcgcgc gccagtgcgc
    24541 accagtcgat gtcacattca cgcatcgctt tttctgtcgc ttcgcgggaa atacctttct
    24601 gattcagttc ctgacgaata cgcgcaggtc cataaccttt gcggctacgg ctggcgataa
    24661 agcgcgcaac aaatcggctg tcatcgagat agccatgttc atggcaccag gcgataacgc
    24721 gctcgtaatc ttctgccgtg gcatcaatct cttccgggcc atttttgccc ataatcggtg
    24781 ccgcgagttt acgtcgcagt tcttgctcac tgtgatcgcg caccgccaga atgcgtaccg
    24841 cacgatccaa caggcgagca tatgccgggc gacgggacgt tgattctgtc atggcatatc
    24901 cttacaactt aaaaaagcaa aagggccgca gatgcgaccc ttgtgtatca aacaagacga
    24961 ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga aatccggcgt
    25021 tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt tcgcggtttc
    25081 cgggttatct ttcagccagg cagtcgcatt cgctttaccc tgaccgatct tctcaccttt
    25141 gtagctgtac cacgcacctg ctttctcgat cagcttctct ttcacgccca gatcaaccag
    25201 ttcgccatag aagttgatac cttcgccgta gaggatctgg aattcagcct gtttaaacgg
    25261 cgcagcgatt ttgttcttca ccactttcac acgggtttcg ctacccacca cgttttcgcc
    25321 ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga atttcagcgc
    25381 gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca tacggatctg
    25441 gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct tacgcatcgc
    25501 ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc cttcgatttc
    25561 cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg caccagaacg
    25621 cgccagggcg tcacagattt ccagtgcctg ctcgccagtg tccggctggg agcacagcag
    25681 gttgtcgata tcgacgccca gtttacgtgc atagattggg tccagcgcgt gttcagcatc
    25741 gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca acgtcagcgt
    25801 ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg gcagaccacc
    25861 tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca catccatgga
    25921 acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa tctggcccag
    25981 tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc ctgtcatgcc
    26041 gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtatg ctcatacagt
    26101 atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca gtagcctgac
    26161 gacgcaccgc atcacggtca ccgctgaagc attcccgccg ggtaatacct tcgccgcggg
    26221 cggtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg ccatccggcc
    26281 cggcgatacc actaatagac acagcataat cagcacgagc cgctttcagt gcgcctatcg
    26341 ccatttccac cacgacaggt tcgctcaccg cgccatgctg cgccagcgtc tcttcgcgta
    26401 caccgatcat ttgcgctttc gcttcgttac tgtaggtgac aaatccgcgt tcaaaccagg
    26461 cggaactgcc ggcaatatcg gtaatcactt tcgctaccca accaccggta caagactcag
    26521 cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg cttaactgca
    26581 tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg gacaagatag
    26641 cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt cagaaaaaag
    26701 ctgattagcc agagggaagc tcacgctccc ctcttgcaaa tagttactgc acgcgcgcca
    26761 gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtactg tggttgtaac
    26821 gggtgatggt gtagaagttc ggcagaccgt accagtactg gtaaccggta ccgacatcca
    26881 gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt aaacctgccg
    26941 cggcaagctg cgaaatgctg tacttggttt tgaagccatt tggcaagcct ggagcctgac
    27001 cgtttgccat caccgcaacc tgatcacctt tcacccagcc gtgcgctttg aaatagttcg
    27061 ccacactacc aatggcatca accggatccc acaggttgat atgcccgtcg ccgctgaaat
    27121 ctaccgcgta ttgtttgtaa gacgacggca taaactgtcc gtagcccatc gccccggcaa
    27181 aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc aggaaagttt
    27241 ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc gttgccagcg
    27301 catcgaggat gcgagttttc cccatcacgc gcccccagcg cgtttcaacg ccgataatcc
    27361 cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcagc gcatcttcat
    27421 actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat tttttgcgat
    27481 aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt ggtgcctggt
    27541 tatccatcag ccgcagtacc gaatccagac gtttcgcctg ggagagaatt tcctgcaact
    27601 gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg gcattcgggt
    27661 tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc aggaagccgc
    27721 cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg ctacaggcgg
    27781 caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg aggggtccat
    27841 ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca aggaagcggt
    27901 agtcactgcc cgatacggac tttacataac tcaactcatt ctcctcgcta tccttttatt
    27961 taaactttca agttaaaata tttatctttc attttgcgat caaaataaca cttttaaatc
    28021 tttcaatctg attagattag gttgccgttt ggtaataaaa caataaatcc tgaaggagag
    28081 aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt tccaaaaggg
    28141 cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta gcctgctggt
    28201 tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt ttgctcaacg
    28261 ttgcgccggt aaccctgttt cccgttacct gctgctaccg tgcattggca cgtttgtctt
    28321 ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaaataca aacccagcta
    28381 ctacgcggcc gcctcttata gctgccactc aatgaatggc ctcttccccc atatcaaccc
    28441 aggcgaactg tttgtttatc ttggcattgc cagcggtctg acaacactga acctgccact
    28501 tggcccactg gcggtgagtt atctgctggt tggtctggtc accaacttct tccgcggctg
    28561 ggtgaccgat ctgaccaccg ccatttttga gaaaaagatg ggcattcaac ttgaacaaaa
    28621 agttcacctg gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg aacgggtggc
    28681 tggggcggcc cgcttgagct ggaagccacg ccgggcaaaa aaatcgtcta tatcaccgcc
    28741 ggtacccggc ctgcgattgt tgacaaactg gcacagctta ctggctggca ggctattgac
    28801 ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat cgactgtggc
    28861 ggcacattac gctgcggcat ttatccgaaa cggcgtattc ccaccattaa tatccattcg
    28921 acgggcaagt ccggtccgct ggcgcagtat attgtggaag atatttatgt ctctggcgtg
    28981 aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc cgtgggccgt
    29041 gactatgaca ccagcaagaa aatcaccgaa caaagcgatg gtttactggc gaaggtggga
    29101 atgggtatgg gttctgccgt tgccgtgttg tttcaatctg gtcgtgacac catcgacact
    29161 gtattaaaaa ccattctgcc gtttatggcg ttcgtctcgg cgcttatcgg catcattatg
    29221 gcttccggtc ttggcgactg gattgcccac ggtcttgctc cgctggcgag ccatccactg
    29281 gggctggtca tgctggcgct catctgctcc ttcccgctgc tttcaccttt cctcggccca
    29341 ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg cctcggcaat
    29401 attccgccgc atctggcttt acctgcactg tttgccatca acgcgcaggc ggcctgcgac
    29461 ttcatcccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg cgtcggtgtc
    29521 ccttctgttc tggtgagccg ctttttaacc ggcgcgccaa ctgtactgat cgcctggttt
    29581 gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc agaccaccat
    29641 cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca tcacctttcg
    29701 tgaaggcgcg cctgcggatc tcgaagaata ttgcttcatt cattgccacg gcgagttgaa
    29761 aggtgcactc catcccggtt tgcaattttc gctcgggcag catcgctatc cggtgaccgc
    29821 cgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc tgcgcttcga
    29881 tggtttaaac gaagcggaat ttccgggcac tgtccatgtg gcaggccctg tccccgacga
    29941 tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa tgaatcaggt
    30001 tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg gtctggctgc
    30061 cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa atgtggcaca
    30121 agaaattaac gccgaatatg gtgaaggaac agcgtatggt tttggtgctg acgccaccag
    30181 cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttggtc gcgtggattt
    30241 gctggtctac agcgccggaa ttgcaaaagc agcctttatc agcgacttcc agctcggcga
    30301 ttttgaccgt tcgctacaag tgaatctggt gggttatttc ctgtgtgccc gtgaattttc
    30361 gcgtttgatg atccgcgacg ggattcaggg gcgcattatt cagatcaact cgaaatccgg
    30421 caaagtgggc agcaaacaca actctggtta tagcgccgcg aaatttggtg gcgtcgggct
    30481 gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt cactgatgct
    30541 cggtaatctg ctgaaatcgc cgatgtttca gtcgctattg ccacaatatg cgaccaagct
    30601 gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc tcaaacgcgg
    30661 ctgcgattat caggatgtgc tgaatatgct tctgttctac gccagtccta aggcgtcgta
    30721 ctgcaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat caacagcgga
    30781 gatccgttaa ggatctccgc gagactaata aaatgcctga tgcgctacgc tcatcaggcc
    30841 tacaggactt ccgccactac attaaggaaa agttatggta tccgcactca tcaccgtcgc
    30901 cgttatcgcc tggtgtgcgc aactggcctt aggcggttgg caaatttctc gttttaaccg
    30961 tgctttcgac acactatgcc agcaagggcg ggttggcgtg ggccgttcca gcgggcgctt
    31021 taaaccgcgg gtcgtggtcg ccatcgcgct agacgatcag caacgcatcg tcgacacctt
    31081 gtttatgaaa ggactgaccg tcttcgcccg accgcaaaaa attcccgcaa ttaccggtat
    31141 gcatgcgggt gatttacagc ccgatgtgat ctttccccat gatccactat cacagaatgc
    31201 tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt acttgcttgc
    31261 gaagttatca ttttgaaacc taaatcaggt aatcacgccc atgaaacctc gtcagcgtca
    31321 ggccgccatt ctggagtatc tgcaaaagca gggtaaatgc tcggttgaag aattggcgca
    31381 atactttgac accacaggca caaccatccg caaagatctg gtcattctgg aacatgccgg
    31441 aaccgtcatt cgtacttatg gcggagtggt gttgaataaa gaggaatccg atccgcctat
    31501 cgatcataaa actctcatca acacccacaa gaaagagctg attgcagaag ctgccgttag
    31561 ttttatccat gatggcgatt cgatcattct tgatgctggc agtaccgttt tgcagatggt
    31621 tcccctgctc tcgcgcttta ataacatcac agtgatgacc aacagcctgc atatcgtcaa
    31681 tgcgctatcc gaactggata acgaacaaac tatcctgatg ccaggcggaa catttcgtaa
    31741 aaaatcggcc tcatttcacg ggcaactggc agagaatgcc ttcgagcatt tcaccttcga
    31801 taaattgttt atgggcaccg acggcatcga tctcaatgcg ggcgtaacca cgttcaacga
    31861 ggtttatacc gtcagtaagg caatgtgcaa tgccgcgcgc gaagtgattt tgatggcaga
    31921 ctcatcaaag tttggccgta aaagccccaa cgtagtttgc agtcttgaaa gcgtcgataa
    31981 gctaattacc gacgcaggta tcgatccggc gtttcgtcag gcgctggaaa agaaagggat
    32041 cgatgtgatc ataaccggag agagcaatga gtgaagcact actgaacgcg ggacgtcaga
    32101 cgttaatgct ggagttacag gaagcaagcc gtttaccgga acgtctgggc gatgattttg
    32161 ttcgcgccgc caatatcatc ctgcactgcg aaggtaaagt ggtggtttcg ggaattggca
    32221 aatcgggcca cattggtaag aaaatcgccg caacgcttgc cagcaccggc actcctgcct
    32281 tttttgtcca tccggcagag gcattgcacg gcgacctggg gatgatcgaa agccgcgatg
    32341 tgatgctgtt tatctcttac tccggtggcg cgaaggaact ggatctgatt attccgcgtc
    32401 tggaagataa atctatcgcg ctgctggcga tgaccggcaa accgacgtca ccgctgggcc
    32461 tggcggcgaa agcggtgctg gatatttccg tagaacgcga agcctgcccg atgcaccttg
    32521 cgccgacttc cagcactgta aataccctga tgatgggcga cgcgctggcg atggcagtca
    32581 tgcaggcgcg cggttttaat gaagaagatt ttgcccgttc acatccagcc ggtgcgctgg
    32641 gcgctcgctt gctgaacaaa gtgcatcatc ttatgcgtcg tgacgatgcc atcccgcagg
    32701 tggcgttaac cgccagcgtg atggacgcga tgctggaact tagccgcact ggtctggggc
    32761 tggtggcggt atgtgacgct caacaacagg tacaaggcgt ctttaccgac ggtgatttac
    32821 gtcgctggct ggttggcggc ggcgcactca ccacgccagt taatgaagcg atgacgacag
    32881 gcggcaccac attacaggcg caaagccgtg ccatcgacgc caaagagatc ctgatgaagc
    32941 gcaaaatcac tgccgcaccg gtggtggatg aaaacggcaa actcaccggc gcaattaacc
    33001 tgcaggattt ctatcaggcc gggattattt aatccttcag ccccagacgt ttcgccagcc
    33061 gatgcaggtt ggcgacgtcg gtttccagca tccgcgcaca ggcggcccag ttgtgatgat
    33121 tttgcgccag cgcctgacga atggtttcac gctggaacgc ttctgtcgct tcacgcaggt
    33181 tttgcttaac aacgggcacc gccgccactt ctggcggcgg caacgtcacc tcaggaaaag
    33241 caaaatgttg cgcctcaaga atcacttcat cgccgctgcg ggttgctctc gccagcacta
    33301 ccgcccgatg aatagcatgt tccagttcac gaacgttccc cggaaaattg tagtgttgca
    33361 gtaaatttcg cgctccggca cttaatacca cgcgggagag ccccagccgc aaacgacact
    33421 gctcgcagaa ataccccgcc agcagaatga catcatcgcc ccgctcacgc agcggcggca
    33481 ctgaaagtgg aaacacgctc aggcggtgga acaagtcagc gcgaaatcgc cctgccagta
    33541 cctcttcgcg taagtcacgg ttagtcgccg ccagcacgcg cacatcgacc cgcaaactgc
    33601 ggtcatcgcc aacgcgctga atatcgccat actgcaacac ccgcagcagc ttggcctgca
    33661 atgccaacga caactcgccg atctcatcga gaaacagcgt gccgttatcc gccatttcaa
    33721 acttcccgct gcggttactg atagcgccag taaacgctcc tttcacatgc ccgaacagtt
    33781 cactttccgc cacactttcc ggcagtgcgg cacagttgag atagaccagc ggattcaccg
    33841 cccgtggcga ggcttcatga atcgctttcg ccaccagctc cttaccggtt cccgtctcac
    33901 cgctgatcag gacgttgaga tcggacgccg ccacaatctc aatctctttt ttcaattgcg
    33961 tcatacctgg cgacagaccg atcatctgcg tctgtttcac tgcttcaaac ggcgcggcat
    34021 cgcctggcag catattctga ctttccagtt gttcaatcag caacgcattg cttaacgctc
    34081 ccgccgccag cgcggcaatc agccgtaact cttcgtcact gaaaacatcg aactgatcgg
    34141 gctgcatccc gtcgagcgtc aatgcgccga tcaggttttg tccggcaaac aatggcagac
    34201 caacgcaggc gtgaaccttc agactctcct gcccgggaat caaaccgtca tagggatcgg
    34261 gcaattcgct gtctgcggga aagcgcacca cgtccccggc gcgggcaatc gcttccagcc
    34321 gtggatgccc ttccagcgca aagcgtctac cgagtacatc cttcgccagc ccgtcgatgg
    34381 caagcggaat aaactgccgc gagtcataac gtagcaacgc agaggcatca cactccagca
    34441 cctgacgcag cgtggtgatc aggcgctgaa aacgatcctg atgaccaatc ccacgctgca
    34501 attcgatggc gatattcgcc agcacatcaa cggaaaaact catctttgcc tcactgtcaa
    34561 tttgactata gatattgtca tatcgaccat ttgattgata gtcattttga ctactcatta
    34621 atgagcataa ttttatttat agagtaaaaa caatcagata aaaaactggc acgcaatctg
    34681 caattagcaa gacatctttt tagaacacgc tgaataaatt gaggttgcta tgtctattgt
    34741 ggtgaaaaat aacattcatt gggttggtca acgtgactgg gaagtgcgtg attttcacgg
    34801 cacggaatat aaaacgctgc gcggcagcag ctacaacagc tacctcatcc gcgaagaaaa
    34861 aaacgtgctg atcgacaccg ttgaccataa attcagccgc gaatttgtgc agaacctgcg
    34921 taatgaaatc gatctggcgg atatcgatta catcgtgatt aaccatgctg aagaggacca
    34981 cgccggggcg ctgaccgaac tgatggcgca aattcccgat acgccgattt actgtactgc
    35041 caacgctatc gactcgataa atggtcatca ccatcatccg gagtggaatt ttaatgtggt
    35101 gaaaactggc gacacactgg atatcggcaa cggcaaacag ctcatttttg tcgaaacgcc
    35161 aatgctgcac tggccggaca gcatgatgac ttacctgaca ggcgacgcgg tgctattcag
    35221 taacgatgct ttcggtcagc actactgcga cgagcatctg ttcaacgatg aagtggatca
    35281 gacggagctt ttcgagcagt gccagcgtta ctacgccaat atcctgacgc cgttcagccg
    35341 cctggtaacg ccgaaaatta ccgagatcct gggctttaac ttaccagtcg atatgatagc
    35401 gacctctcac ggcgtggtat ggcgcgataa cccgacgcaa attgtcgagc tgtacctgaa
    35461 atgggcggcg gattatcagg aagacagaat caccattttc tacgacacca tgtcgaataa
    35521 tacccgcatg atggccgatg ctatcgccca gggtattgcg gaaaccgacc cacgcgtggc
    35581 ggtgaaaatt ttcaacgtcg cccgaagcga taaaaacgaa atcctgacca atgtcttccg
    35641 ctcaaaaggc gtgctggtcg gcacttctac gatgaataat gtgatgatgc cgaaaatcgc
    35701 cgggctggtg gaggagatga ctggtttacg cttccgtaac aaacgcgcca gtgctttcgg
    35761 ctctcacggc tggagcggcg gtgcggtgga tcgtctttcc acacgcctgc aggatgcagg
    35821 tttcgaaatg tcgcttagcc tgaaagccaa atggcgaccg gaccaggacg ctctggagtt
    35881 atgccgtgaa cacggtcgcg aaatcgcccg tcagtgggcg ctcgcgccgc tgccgcagag
    35941 cacggtgaat acggtggtta aagaagaaac ctctgccaac acgacggctg acctcggccc
    36001 acggatgcag tgcagcgtct gccagtggat ttacgatccg gcaaaaggcg agccaatgca
    36061 ggacgttgcg ccaggaacgc cgtggagtga agtcccggat aacttcctgt gcccggaatg
    36121 ctctctcggc aaagacgtct ttgacgaact ggcatcggag gcaaaatgag caacggcatt
    36181 gtgatcattg gttcgggctt cgccgcccgc caactggtga aaaatattcg caaacaggac
    36241 gccagtattc cattaactct gattgccgcc gacagcatgg atgagtacaa caaaccggat
    36301 ctcagccatg ttatcagcca ggggcaacgt gccgatgacc ttacccgcca gacggcgggt
    36361 gaatttgccg agcagtttaa tctgcacctg tttccgcaca cctgggtgac ggatatcgat
    36421 gccgaagccc atgtggtgaa aagtcagaat aatcagtggc aatacgacaa gttagtgctg
    36481 gcaaccggag ccagcgcctt tgtcccgcca gtgcctgggc gtgagttaat gctgacatta
    36541 aatagtcagc aagagtatcg cgcctgtgaa acgcaactgc gggatgcccg acgcgtattg
    36601 attgttggcg gtggcttgat tggtagcgaa ctggcgatgg atttttgtcg tgcaggcaaa
    36661 gcggtcacgc taatcgacaa cgctgccagt attctggcat cgttaatgcc accggaagta
    36721 agcagccgct tgcagcatcg gttgacggag attggcgttc atctgctgtt aaaatctcag
    36781 ttgcaggggc tggaaaaaac ggattctggc attctggcaa cgctggaccg ccagcgcagc
    36841 atcgaagtgg atgcggtaat tgccgccacc ggactgcgcc cggaaaccgc cctggcacga
    36901 cgcgccgggc tgacgattaa tcgcggcgtt tgcgtcgata gttatctgca aaccagtaat
    36961 gccgatattt atgcgctggg cgattgcacg gaaattaacg gtcaggtatt gccgttcctc
    37021 cagccgattc aacttagcgc gatggtgctg gcaaaaaatc ttctcggcaa taacacgccg
    37081 ctgaaactcc ctgcgatgct ggtgaaaatc aaaacgccag aattaccgct gcatctggca
    37141 ggcgaaaccc agcgtcagga tttacgctgg caaattaata ccgaacgcca gggaatggtg
    37201 gcgcgcggtg ttgatgatgc cgaccagctt cgcgcctttg tggtcagtga ggatcggatg
    37261 aaagaggcgt ttggattgtt gaaaacgttg ccggtgtagg catggttttc tccctcgccc
    37321 aaagggcgag gggactgtcc gcgctctttt tacgcttagt tacgccacat ccgccactat
    37381 gcagcatgca ggatgcgcgc cgcagcaata accccctgcc ccagagacaa gccgccatca
    37441 cccgccggta aactctgcgg aaagagtaat gtgaaatcag cgagataatg cgccaggcgt
    37501 gtacgcagca atcggttatg aataacccca ccgctaaata ccagcgtagt gataccgcgc
    37561 atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc atgaaacgcc
    37621 cacgcgcgtt gattaaccgg tgcctgccag ttcagccact gctgccagaa agtggcgaga
    37681 tccagttgat tgtccaccag cggcatcgtc accggatgcg tcactccgtc gcatgaggct
    37741 gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc tggcgcacag
    37801 cccagtgccg ccgccacagc atcgaacaaa cgcccacacg atgacgccag cggcgcgtta
    37861 attccacgct caatggcccg cgccagcacg ctccagtttt gctgttgcac acttgtcgtt
    37921 tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag gttacgccac
    37981 ggctgctttg cagccaaatc gccccccgga agcgccaccg caggcaagcc gcccaggtgc
    38041 tcacattcgc gatagttcac ccgcaggcat tcgccgcccc acaaagcgcc gttctccccc
    38101 ataccgatac cgtcgagcgt caacgcaatg acatcaccgc catccagcgg ccactgatgc
    38161 tctgccagac tcgccgccgc atgagcatga tgatgcagca ccgtttgcgt cggcagattc
    38221 atttcgcgtg cccactggct ggagacatag cccggatgcg cgtcatgcac aacgtattgc
    38281 ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg catctggatg
    38341 ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc gcgcaccagg
    38401 cagaaggtgt ttttcagatc cgcgccgaga cacagcacag gcggaacatt tttaaagccc
    38461 ggaggcaaag ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat ttcgccgctt
    38521 tcgcgcacca ccgaatcatc catccgctgc acgatgtcgc ggttatgtag caagaatccg
    38581 tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagccgg tggtttaccg
    38641 ctcaggttgc cggaggtcat caccagcgga cattgcagtt cctgtaacag caaatgctga
    38701 agcggattcg caggcaacat caccccaact tcgttaaggc caggggcgat atcatcacaa
    38761 agctcaggaa cgtatttttt atccaccagc acaatcggcg cggcgggtgt ggtgagcaac
    38821 tggcgcgcag cgtctggtaa accttctgcc actggcaaca tgaccgccag cggtttcgcc
    38881 gggcgatgtt tgcgcgcccg aagtgtcgcc accgcgttac tgttacgtgc atcgcaggca
    38941 agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa ctgtgcgata
    39001 gctgcctgta atgccgcctc ttgttccgca tgttcaccat gacttaccca ttcaagatgt
    39061 gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag cgggtcgcgg
    39121 tactctttgt cacaagccgg acacaacgga aacgccgcca tcacggtaaa cgggcggtcg
    39181 taaggcatgg cgcgaataat ggtgaaacgc gggccacagt gagtacagtt gataaacgga
    39241 taacgataac gccgctcgcc tggggtattc atttcggcaa gacaggcagg gcaagtagcg
    39301 gcatcgggaa caatttgcgt attcatggtg ccgcctgtgc tctggcgtat agtgaactcg
    39361 gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg cgccagcggc
    39421 gggcagtgct gatgcaactg cacaagaaac gtttccgggt cttccagcag ccggacttct
    39481 acgccatcgc cgtcattaca gacatcgccg tgaagattta attgctgtgc cagctgccag
    39541 acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg gacaccgcaa
    39601 gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc cgccctgccg
    39661 gacacgacag cgtcgtatcc ggcaatcaca ggtcggcgat accgcccgct ccgtattcta
    39721 cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt tagaacatca
    39781 gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga tcgacacaaa
    39841 tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga tggttacaga
    39901 ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagccctgcg ccgctgttgc
    39961 gaatcaccgg acgtaccacc acttccatcg cgccatacgg gcaagccaca acgcaggttt
    40021 tgcaaccaat gcaacgttcc tgcatcacat gaacaaaccc tttatcacgg ctgatagcac
    40081 catttgggca gacgttagcg cacggtgcat cttcacactg acggcagact gttgccgtag
    40141 aaatgttcac acctttaatg acatggattc gcggtaaaaa agtttccggg gtcagcgatg
    40201 cacagtcctg attctcctga tgagaaacca cgcacgccac ttcacaggta cggcaaccaa
    40261 tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc aatgacagtt
    40321 aatgcgccga tacattcaca aatcatgcca gtttttaatt tactgttatt taaagaaatt
    40381 aaattctgta atgcaggaaa aacgatgtca tcgacactag tgacgatgac atgtgatgac
    40441 aatgtttatt gcgaaagagc aatgagggag ccgcggcgga tcagttttcc gctgaaggtt
    40501 ttcggcggtg agaaatcccc accatcgagc ataaaaatca gccgtccaat aatttcctga
    40561 atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata gggggcaatg
    40621 gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc gcgctcgtgt
    40681 aacgctttca tcgcacctat cgccatatcg tcgttactgg caactaacgc gctaaattta
    40741 gccccacgtt cgagcaacgt ttctacccct tcggccccgc tggcaggcgt ccatttaccg
    40801 ttagcgataa gtttttcatt gagcgcaata ctatgctgcg ccagcgcgtc tttatacccg
    40861 gcaagacgtt caatgctggt gggagaatcc atcgagccgg taaggaaagc aatctcctga
    40921 tgtccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg atcgcaccag
    40981 acgctatggc tgctgttttt gcgcaggcgg cgattaagca ccattatcgg ctgactgtgt
    41041 gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat cataatggcg
    41101 tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc gctgtgttta
    41161 ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc atgaaagagt
    41221 aattcactaa aataaatgcc gtggtaaagc gtgttggtca cgaccagccc cagcgtctga
    41281 gtactcttcg ccgacagatt gcgcgccagc aagtttgggc ggtaaccgct ctcttctacc
    41341 gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga aagcacgcgg
    41401 gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt
    41461 ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt gtccttgcgc
    41521 aatcggcggg aaaaatattc aggtgaccgg tttcacaaat ataaaaaatg aacaattaac
    41581 tctcttgctt atttagtgac aactattcat gattttgtga aaccggtttc ctaattccgt
    41641 ttcagcatcg gcatttttcc gtcacgtcga ctgataacaa ctacatctac cctactgata
    41701 acaggataaa atccgatggc caaaaattat gcggcgctgg cacgctcggt gatagcggca
    41761 ctgggcggcg ttgataacat ctcggcagtc acgcactgta tgacgcgttt gcgctttgtt
    41821 atcaaagatg accagcttat cgacagtccg acgttgaaaa ccatctccgg cgtgctcggc
    41881 gtggtacgta gtgacaacca gtgccaggtg attatcggca ataccgtttc acaagccttt
    41941 caggaagtcg tcagcctgct gccgggagat atgcagcccg cacagcccgt gggtaaaccc
    42001 aaacttacgc tacgtcgcat tggtgcgggg atcctcgatg cgctgatcgg caccatgtca
    42061 ccgctgatcc cggcgattat cggcggatcg atggtcaaac tgctggcgat gatcctcgag
    42121 atgagcggcg tgctgacaaa aggatcgccg accttaacca ttctgaatgt gattggtgac
    42181 ggtgctttct tcttcctgcc gctgatggtc gcggcttctg ccgccatcaa atttaaaacc
    42241 aatatgtcgc tggcgattgc catcgcgggt gtgctggtac atccgagttt tattgaatta
    42301 atggcgaaag cggcccaggg tgaacatgtc gaatttgccc tgattccggt caccgcggtg
    42361 aaatacacct acacggtgat cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa
    42421 cgctgggtgg acagcattac accggcggtg actaaaaact tcctcaagcc aatgctgatt
    42481 gtgttgattg ccgcaccgct ggcaattctg ctgattggcc cgattggtat ctggatcggt
    42541 agcgccattt cggcgctggt ttacaccatt catggttatc tgggctggct ttcagtcgcc
    42601 attatgggcg cgctgtggcc tctgctggta atgaccggga tgcaccgcgt ctttacgcca
    42661 accatcattc agaccattgc cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc
    42721 ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg cgtggaaaac gaaaaacccg
    42781 gaactgcgcc agacggcgct ggctgcggcg gcatcagcca ttatggcggg gatttccgaa
    42841 ccggcgttat atggcgtggc gatccgcctg aaacgtccgc ttatcgccag tcttatcagc
    42901 ggttttattt gcggcgcggt tgccggtatg gcggggcttg ccagccactc aatggcagcg
    42961 ccggggctat ttaccagcgt gcagttcttc gatccggcga atccaatgag catcgtctgg
    43021 gtgttcgcgg tcatggcgct ggcggtggtg ctgtcgttta tcctcacact gttgctcggc
    43081 tttgaggata ttcctgttga ggaagcgact gccgaggcgc gaaagcatca gagcgcacaa
    43141 ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa aatgtcagta tttccagaag
    43201 gttttttatg gggcggcgcg cttgccgcca accagtctga aggtgcgtac cgtgaaggtg
    43261 gcaaagggct gaccaccgtc gatatgatcc cacacggcga gcatcgaatg gcggtgaaac
    43321 tggggctgga aaaacgtttt cagttgcgcg atgacgagtt ttatcccagc catgaggcga
    43381 cggattttta tcatcgttat aaagaagata tcgccctgat ggcagagatg ggattcaaag
    43441 tgttccgtac ctcaattgcc tggagccgcc tcttcccgca gggcgatgaa ctgacgccca
    43501 accagcaggg cattgctttt taccgttctg tctttgaaga gtgtaaaaag tacggtatcg
    43561 aaccgctggt cacgttgtgc cacttcgatg tgccgatgca tctggtcacc gaatatggct
    43621 cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta cgccagaatc tgctttgaag
    43681 catttgatgg tctggtgaaa tactggctaa ccttcaatga aatcaacatt atgttgcata
    43741 gcccgttctc cggcgcgggt ctggtgtttg aagaaggcga aaatcaggat caggtgaaat
    43801 atcaggctgc gcatcaccaa ctgattgcca gtgcattagc caccaaaatc gcccatgagg
    43861 ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg taacttctac ccttacagtt
    43921 gcaagccgga agatgtctgg gcggcgctgg agaaagatcg ggaaaacctg ttttttatcg
    43981 atgtgcaggc gcggggcgcg tatccggctt actctgcccg cgtattccgc gaaaaagggg
    44041 taaccatcga caaagcaccg ggcgatgatg agatcctgaa aaacaccgtc gattttgtct
    44101 ctttcagcta ttacgcctca cgctgcgcct cggcggagat gaacgccaac aatagcagtg
    44161 cggcgaacgt agtgaaatcg ctgcgtaacc cgtatctgca agtgagcgac tggggctggg
    44221 gtattgatcc actcggtctg cgtatcacca tgaatatgat gtacgaccgt tatcagaagc
    44281 cactgtttct ggtggaaaac ggcctcggcg caaaagatga atttgctgcc aatggtgaga
    44341 ttaacgatga ctatcgcatc agctatttac gcgaacatat ccgcgcaatg ggcgaagcga
    44401 ttgcagatgg cattccgctg atgggctaca ccacatgggg ctgtattgat ttagtttccg
    44461 cctctacggg tgaaatgagc aaacgctacg gttttgtcta cgtagaccgt gacgacgtag
    44521 gcaacggcac gctgacgcgc acacgtaaga aatcgttctg gtggtataaa aaagtaattg
    44581 ccagtaatgg tgaagattta gagtaagtaa ctaccgtcgc acgaaccagg aaagcccgat
    44641 aagcgttgcg catcgggcaa tcttgggttt ggcatcagtc aaaatcaaag aaaccattcc
    44701 ggcttaacgc caaatcgggc tgaaagtgcc ttaatatggg atatcgtcag ggatcgttgc
    44761 ccagacaaaa tctgactgac cagagattta gagccgattt cgttcttcaa atccgcatag
    44821 gtcaaattat gttgatcaat cagcgtacga agtaaggcca cgccaaccgg cattgctgca
    44881 acggctttgt cgaactctgc aaacttttcg ttgttgtttt catattctgc aatgcggctc
    44941 gccagaaagt cgataagtgg attggtgtca tcttcttcaa tcaaatattc gaccagttcc
    45001 agtgcttcac gataatcatc ttctgagtca ctgcctccca gaaaggggac tgcattgacc
    45061 agttctcttg ttgctttaac agcgcgtgca gcattagcag tcattctttg ttctctctgt
    45121 agtaacgtgt cagtttgtca tactctgcgt gattcgttat gtgcttcaca aagaaccgct
    45181 tattaacaaa gtttatgtag gcaataaccc taagattgtt cccgccgaca tccagaacgt
    45241 accatttgtt gcgatattta aaattgtcga ggttaggaaa cgccgttcgc atttcttctg
    45301 gtgtagaaaa atccgtttcc ttaataaccc gatacaatga gtgtagagcc agagcgtcgt
    45361 taggatattt gcgcgcacac tcttcaaatg gcgctttcga aatgatgtgc attttaactt
    45421 ccttattagc gcctgaatga ctaaacctgg actaccattc cttcggattt atttttctat
    45481 agtagttgat cacacgggtg ttcacaatat gtaaacaccc gtctacacgt cacaattgca
    45541 cgtccaaatt ccaaaacgtg aacatgtttg tctacgaact actcttcttc caccgctaac
    45601 tgcgcgaatc cgccattccc ttcccagcct tccagtcgtt gataaacggt ttctacggcg
    45661 tctttaatcg gctgggtcat cgggtagtaa aagccgacga tatccggctg gatgccgagg
    45721 aaaatcactt cgccaatgtc ttctttcaac tggtcgataa ggtagttcaa cggcatgtta
    45781 tgggtagtca tcataaacat ctcggcgata tcatccggat cgatgatgcg gatctcgccg
    45841 gggtttagcc ccatatccgt ggcgtcgaca atcaacagtc gtgtcgggcg cagttcgcga
    45901 atagcgacga tgtcgttttc cggtgcgcta ccgccgtcaa tcaccaccca gttacctttc
    45961 ggcgcggcgg cacacttttc cgccagcagc ggacctgcgc catcatcgcc catcatgcta
    46021 ttgccaacac agagtaaaac gtcagtcacg caatctcctc accatcaaat agatggcgtt
    46081 ttcctgatga atatcatgca gcatacttaa cagcattgta ctccattctt tctgctgcga
    46141 agtttgcgca gcccgggctt tatcgaatgc gttggtcagc atcggcacat ggttaatgtc
    46201 gatgacgatc tcaccatatt tcggcacgcc ttccattttg cggcgggctt cgctgcccgc
    46261 ctccagagtg gcaatccatg ccagatattc atcccacgga caggtgagcg ccgcttccag
    46321 gcaatcgata accccaaggt ggtgaccaat cgccaggctg taatagacca cctgctgcgc
    46381 ctcggcgggc gtggcatcgt tctcatcaat aaatttacgg ctcagttgac tgaacaccac
    46441 cttttcactc atcggatacg cgcctcttca acaacatgat tcagatggct gacaatctcg
    46501 ttcagacgcg gatcgttttc cgcttccagc cagcgtgcaa cctgctcttc gccctgccct
    46561 aactgtgtca ggaagtcatc ggcaatctgg cgaccgtaac ggtaacccgc caggcggcga
    46621 gcttcgcgat ccactttcac gcgcagcggc tgcaccatat cgccatgcag gatctccgcc
    46681 ggttgttcat cctgctcacc cggcccacgg gcgtgaattt tctgctccag caggccgagc
    46741 gccattgcaa agccgtacag cgtggcggca ggcgttggcg ggcagccagg gatataaaca
    46801 tccaccggga cgattttatc cgtaccgccc cacacgcagt agagatcgtg gaagatcccg
    46861 ccgctgttac cgcaggcacc gtaggagata caaattttcg ggtccggcgc ggactgccac
    46921 gcacgcagcg caggggatcg cattgcacgg gtgaccgcgc cggtaaacag taaaatatcc
    46981 gcatgacgcg gtgaaggaac gactttaatg ccgaagcgtt ctgcatcaaa cagcggcgaa
    47041 agcgtggcga aaatttcgat ttcgcagccg ttgcagccgc cgcagtccac gcggtaaacg
    47101 taggcagaac gtttgatttt tttcagtaac gacgccttca tgctggcgat ggattcatcc
    47161 accgtcatcg ggaccggaat gccgttggcg tcacgggggc ctaataaatt gctcatcaga
    47221 tggcctcttt catatggcga gtcagttcaa tacggtcgga cggcaccagg catttctggc
    47281 gcttacattc cgggcaagtc tcaaagcttt cgcggtggtt ttccgcgcgg ctgtcgccgt
    47341 tgtgcttgag cagcgcaatg gcgtagtcga tctctttctg gacggcgaaa ggacgattgc
    47401 agacgcgaca gttgcacagc gcgaagcggg actgttgcag gaagtcttct ttcttccaca
    47461 ccgccagttc gtactcttgc gacagcttaa tcgccgccgt cgggcaaact tcttcgcagc
    47521 gtccacagaa gatgcagcgc ccgagattaa actcccaggc aagctctcct gtggcgaggt
    47581 cagtttcaac cgttaaggcg tttgacgggc aggcattgac gcaggccgcg cagccgatgc
    47641 actgctgcgg gttctgctct ggcttaccac ggaagttttt atcaaccgca atcggctcca
    47701 gcggataaga cgaggtcgcc gtgccggttt tgataacttt tttgataaag gtaaacatgg
    47761 cgattcctta tttcagcggc gagtttttac gctcaatgct gtaacgctcg agttctttgt
    47821 acggcaccac tttgctcttc ttcttacgca catcgaccac ggtcatgcgg tcggtacagg
    47881 agtagcaagg gtccaggcta ccgataatca gcggcgcatc ggaaacggtg ttgccgcgca
    47941 gcatgtagcg cagggtcggc cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca
    48001 gcttctggtt gtcgccggtc atgctccagt ggatatcgtc gccgcgcggc gcttcggcaa
    48061 agcccagcgc aaaacggtgc ggaatatagg taaagccttc caccatcagc ggaccgcccg
    48121 gcaggttatc cagaccgtag tcgatcatgt tcagcgcggt atagacctcg ttgatacgca
    48181 ctttcagacg ggaaataacg tcgcagccct gctcgctgtg gacttccatt ggcagcaggc
    48241 cataaccaac aaacgggtga tcggcacggg tatcgcgggc gtgaccgctg gcgcggacca
    48301 tcgggccaac gttactgaaa tcgcgggcga tttccgggtc cagacgacca atgccgacag
    48361 tacgctgttc catgttcggt gtgctcagca gcacatccac cagctcctgc acttcacgac
    48421 gcatctgttg cgccaactgg cgggtctgga tcatgtcatc tttcagcaga tcgcgacgaa
    48481 ttccgccgat caggttcagg ccgtaggttt tacgcgcacc ggtaaggatc tctgccattt
    48541 tcatggaggt ttcacgcacg cggaagaact gcataaagcc ggagtcgaag ccggtaaagt
    48601 gacaggcgag gccgaggttg agcagatgcg agtgcaggcg ttctacctcc agcagaatgg
    48661 cgcggatcat ctgcgcacgt tctggcacct gaatacccat cgcgttttcc accgacgtgg
    48721 tgtaggcggt gctgtgagca aagccgcaga tcccgcacac acggtcagag aggaaggtca
    48781 cttcgttgta acccatgcgg gtttccgcca gtttttccat gccgcgatgg acatagaaca
    48841 ggcggtagtc ggcgtcgata atgttttcgc catcgacgaa cagacggaag tgacccggtt
    48901 cgtcggaagt gacgtgcagc ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt
    48961 cgttgatgaa ctcgtaggtt tcagcatcgg tagtcggtgc cggacgttga cgataatcca
    49021 tgctgtcttt acgcagcgga taaagttcat ccggccagtc atccggcagc accagacgac
    49081 gttcatccgg cagccctacc ggaatcaaac cgtacatatc gcgcacttca cgctcgcccc
    49141 ataccgccgc cggaacgcgc ggcgttacgg acggatattc cggtttgttg gcgtcaactt
    49201 caacgcgcac ggttatccag catttggtgc ccttctccat cgacagcacg tagtaaacgg
    49261 cgtaatgacc attaagtttg cgttcgtcgt taccaaacag caccgacagc cagcccccct
    49321 gcttgtagta gagaaactcc accacttccg gcaggtagtt caccttcacg gtcacggtca
    49381 gctgatcttt ggtctgccag gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg
    49441 cagcgagata atgttgacct aatttttctt cagacatgct caaactctct ttaatcacgc
    49501 cgccagcaag gagacgaacg ctaaaaatgc aaagccaaac ccagcccagg taatgcgcgg
    49561 agtaatatca agacgcagac gggccatgct gttttcgaac agcgcgataa ccaggacgcc
    49621 aaccaccagt ttgacgatgg caatcaccag cgccagcagc agcccaccga cggtgaaggt
    49681 ttccatttgt ccccacggaa taaacacccc gacgaacatc tgcaacacca ccagctgttt
    49741 caggctgata ccccatttca tgacgccaaa gccgctgccg ctatactcag agagcggacc
    49801 ttcctgcaac tcctgctcgg cttccgccag gtcgaacggc agtttgccca tttcgataaa
    49861 ggtggcgaat gcacaggcac aaagcgccag caccagcgga atgctctggc tcagcggcca
    49921 gtgataaacg gtgtcggtga tgttgctgat gttggtggaa ccggcaacct gtgcggcgac
    49981 ccacagacca agcagcagca tcggttcaac cagcacgcca agcatcgctt cacggctcgc
    50041 gccgatagcg gtaaacgggc taccggtatc cagaccagaa atggcaaaga agaaacgcgc
    50101 gatggcaaag agatacagta aagtgatcaa atcgcccagc tgcggcagcg gagaaccaac
    50161 ggtcaccacc ggcagcgcag tagcgatagt cagcatgacg cccaccatca catacggcgt
    50221 caggcggaac acccagccgg aggcatccgg gccaacgctc tggcggccca gcagtttgat
    50281 aatgtcgcga tactcctgca acacgcccgg cccgcgacgg ttatgcaagc gggcgcgcgc
    50341 cacgcgggtt ataccggaga gcagcggcgc aacggcaaat aacaccagcg cctgaattaa
    50401 cggatataaa acactcattc tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc
    50461 agttcaacca gcgccatccg gcggaacagc aacgcactcc cctcgcactg ccagcccggt
    50521 accagcgaca ccggattcag ccatttgcgc agtttcagca ccggcgcaaa cgcctgtttc
    50581 accggcatgg caaaaccgtg agcagtaatc accattgatt tttcatggtc gtagccgcac
    50641 acccaggccg cgccgcggga acgcgacggc aaacgatcgc ctttgcaaat cgccataatg
    50701 atgaatggca gcagcgggca ggcaatcagc agcaacgtga tcatgggttg agaaacggtg
    50761 gtgttagcag gctccagcgg cagaggtaca gcagcagaga gcatcggcag cagccacggc
    50821 gcagcaacac cgccaattac gcagcaaatc gccagtgcca ccacgcttac gctcatcagg
    50881 agcggcgcac tggtggcgtt ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg
    50941 ccatagactt tcgccataca catcaccgcc agcgcaccgg taattgccag acccactgcg
    51001 agcagcggcc ccagcagacg ggcaacaaac gcgccactat tgctcagttt gaagaaggat
    51061 tgatagataa cccattcccc ggcaaacccg ttcagcggcg gcaacgcagc cattgccatc
    51121 agcccgacta acatggcgat ggagataacc ggcattttct tgccaatacc accgagtttt
    51181 tcgatatcgc gatgaccggt acggaaccag acgctccccg ccccgaggaa cagtacgctt
    51241 ttgaacaggc tatggttaag cagatggtac agaccaccga ccaggccaag agcaatcagc
    51301 gccggttgtt cgagcgcgat acccgttacg ccagcgccca gccccagcag gataatgccg
    51361 atattctcca gggtgtggta agccagcagg cgctggatgt tgtgttccat caacgcatac
    51421 agaccaccga cgaacgcggt gatcatgccg agcaccagca gcgcgatccc ccaccacagc
    51481 ggtgcattac cgcccagcag cgacagggtt aaaatgccca gcaggccaat tttcatgact
    51541 accgtagaaa acaacgcggc agctggcgca gaggcgttag catgtgcctg cggcacccag
    51601 ccgtgcagcg gaataatccc ggccagcagg ccaaagccaa tcacaccgag cagccagata
    51661 tcggaaccga gcggcagctg ttgcatacgc atatccagca ggcgcagatc cagcgtgccg
    51721 taacgctgcc acagcagcca gcaggcaatc gccagcagca gagtgccaag acgccccagc
    51781 gcaaaccaca gtttgccctc tttgctgttg ctggtgagga acaccgcaca cagggccatg
    51841 atttcggcca ttaccacgaa catgccgagg ttgctggcga tgacggcaca gacggcggca
    51901 gccatcaaca tattgatctg caagccgttg catttcacct gcgcgtggcg atgccagtca
    51961 atgttgtaga ggctgacaaa cagaccgcac aggccgagcg taatcagcca aatcgcgtta
    52021 agcggagaga tttgcacatc gtagcttacc agcgacagcg caccgctcac gccaaccgca
    52081 ccagtcagca cagtgaagcc cgcggctgcc gtatacaggc tacccaccgc gccgccaatt
    52141 ccggctatcc agccgcttag cgccttttga aaagaaaaga gaaatgccag aacagcggcg
    52201 gcggcaaacc acgccacgcc actattgatc agggaaattg cgctcattta gcctctccac
    52261 tttgagcctg ctgaaacaag gtgagatcgc caaagtccgt gttaaaagtc agctcacgct
    52321 tacgtttgct gacgcgggcg atatcggtgt tatccaccag atgcagggct ttagtcgggc
    52381 acatccgcac gcaggccgga ccttgttcat caaagctaca aaggtcacat ttgacggcga
    52441 tcgcgcgaat acctggcacc cagtcaagca atgtgctgac acgcgccgga gcaggcggtg
    52501 ccggtggcgc tttcggggta ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa
    52561 attcaattgc gccaaacggg caggcgatgc cgcacagttt gcagcttacg cacagacttt
    52621 cattcaactg cacggcccca tcaacgcggg tgatggcgtt aaccgggcaa accaccgcac
    52681 agggcgcatc ttcacagtgg tgacagagct gcggcgcaga ttctttttca ttgagcatca
    52741 ctcgcaggcg cggcattgat tgcaggccgt gctggcgatg cgtctctgaa caggcggcct
    52801 cacaggtgtg gcagccgata cagagcgtgg agtcagcaat tacaaaacga ttcaccaggc
    52861 attcctcagg tgattgtcat ttttgacgaa aacatgccga tgaaatgtca ttttcgacac
    52921 tcatcgacac gcccatcccc aaacaggcgt aacgcctgca aaacgggcaa agcctcagct
    52981 catgctgccg ggctttgtcc ctttaccagt tggcttaaat tcaccggcac attgttttca
    53041 atggcggtgt ataagctgtc gtaaacgcca gcgatttttt cgagatagtg ttccagaacc
    53101 tgttcgcgat cgcggatgtt gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc
    53161 tgcgcaatcc gctgtttgtc ttcaccatct tttgtttcga cgtaatactc gatggtgtgg
    53221 ctgttaaagc catccgccag ggtgacgtaa atgcgaaaat gttcaaaaag gacgaaacag
    53281 agttctttgt ccggcgcgca gctcatggcg tgatgcaggc gcgctttcga taacgtgatc
    53341 ccctgaacca gcgaattgca gtagatgtgc cactggtcct gtaggcgacg atgccgctgt
    53401 gcgatgtaat cggctttctc gcttatttcc caaatagtca ttgtcaggtt acccgtttaa
    53461 cagagatgcc agctttaagc attttctgtg ccaactttta attcattgtt atgaaacaga
    53521 tttttaagat aaagatgaat ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca
    53581 ttattcaccg cagggataat caacactggc acaattattg cttgtagctg gcaatagtta
    53641 atgggaggcg atatgcacga aataaccctc tgccaacggg cactggaatt gatcgaacag
    53701 caggccgcaa aacacggcgc aaaacgcgta actggggtct ggctcaaaat tggcgcattt
    53761 tcttgtgtcg aaaccagctc tcttgccttt tgttttgatc tggtttgccg cggcagcgtg
    53821 gcggaaggtt gtaaactgca cctcgaagaa caagaggccg aatgctggtg tgaaacatgc
    53881 caacagtatg tgacgctact gacccagcgc gtccgccgct gtccacagtg tcatggtgac
    53941 atgctacaga ttgtggcaga cgacggttta cagattcggc ggatagaaat agaccaggag
    54001 tgagcgatgt gtacaacatg cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa
    54061 cataaccctc attccgcgtt tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg
    54121 aaaatcaccg gcattaaagc gcctgaattt acccccagcc agactgaaga aggcgatctg
    54181 cattacggtc atggcgaagc gggtactcac gcgccgggca tgagccagcg tcggatgctg
    54241 gaagtcgaaa ttgacgtgct ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc
    54301 tttgctgccc gcaagcaact ggtgctcaac ctggtttcca gccctggttc cggtaaaacc
    54361 accctgctga cggaaacctt aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa
    54421 ggcgaccagc aaaccgtgaa cgatgccgcg cgcattcgcg ctaccggcac accagcgatt
    54481 caggtgaata ccggtaaagg ctgccatctt gacgcacaga tgattgccga cgccgcaccg
    54541 cgcctgccac tggacgataa cggtattctg tttatcgaaa acgtcggcaa cctcgtgtgc
    54601 ccggccagct tcgatctcgg tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt
    54661 gaagacaaac cgctgaaata tccgcatatg tttgccgcag cttcgctgat gctgctcaac
    54721 aaagttgact tgctgccgta tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc
    54781 gaagtcaatc cagaaattga aatcatcctt atttccgcca ccagcggcga agggatggac
    54841 cagtggctga actggctgga gacacagcga tgtgcatagg cgttcccggc cagatccgca
    54901 ccattgacgg caaccaggcg aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa
    54961 cgttagtcgg cagctgcgat gaaaacggtc agccacgcgt aggccagtgg gtactggtcc
    55021 acgttggctt tgccatgagc gtaattaatg aagccgaagc gcgcgacact ctcgacgcct
    55081 tacaaaacat gtttgacgtt gagccggatg tcggtgcgct gttgtatggc gaggaaaaat
    55141 aatgcgtttt gttgatgaat atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct
    55201 gcgcgaacgt gcttcacatc tctcttacac agccgaacgc cctctgcgga ttatggaagt
    55261 gtgtggcggt catacccacg ctatctttaa attcggcctc gaccagttac tgccggaaaa
    55321 cgttgagttt atccacggtc cgggttgtcc ggtgtgtgta ctgccgatgg gtagaatcga
    55381 tacctgcgtg gagattgcca gccatccgga agtcatcttc tgtacctttg gcgacgccat
    55441 gcgcgtgccg gggaaacagg gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg
    55501 catcgtttac tcgccgatgg atgcgttgaa actggcgcag gagaatccaa cccgcaaagt
    55561 ggtgttcttc ggcttaggtt ttgaaaccac catgccgacg acggccatca ctctgcaaca
    55621 ggcgaaagcg cgtgatgtgc agaattttta cttcttctgc cagcatatta cgcttatccc
    55681 gacgctgcgc agtttgctgg aagagccgga taacggtatc gacgcgtttc ttgcgcccgg
    55741 tcacgttagt atggttatcg gcactgatgc ctataatttt atcgccagcg attttcatcg
    55801 tccgctggtg gtcgctggtt tcgaacccct tgatctactg caaggcgtgg tgatgctggt
    55861 ggagcagaaa atagcggccc acagcaaggt agagaatcag tatcgtcggg tggtgccgga
    55921 tgccggtaac ctgctggcgc aacaggcgat tgccgatgtg ttctgtgtca acggcgacag
    55981 cgaatggcgc ggcttaggcg tgattgaatc ttctggtgta cacctgacgc cggattatca
    56041 acgattcgat gccgaagcgc atttccgccc ggcaccgcag caggtctgcg atgacccgcg
    56101 cgcacgttgt ggcgaagtat taacgggcaa atgtaagccg catcaatgcc cgctgtttgg
    56161 taacacctgt aatcctcaaa ccgcgtttgg tgcgctgatg gtttcctccg aaggagcgtg
    56221 cgccgcgtgg tatcagtatc gtcagcagga gagtgaagcg tgaataatat ccaactcgcc
    56281 cacggtagcg gcggccaggc gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt
    56341 gccaacccgt ggctggcaga gcaggaagat caggcacgtc ttgatctggc gcagctggta
    56401 gcggaaggcg accgtctggc gttctccacc gacagttacg tcattgaccc gctgttcttt
    56461 cctggcggta atatcggcaa gctggcgatt tgcggaaccg cgaatgacgt tgcggtcagt
    56521 ggcgctattc cgcgctatct ctcctgtggc tttatcctcg aagaaggatt gccgatggag
    56581 acactgaaag ccgtagtgac cagcatggca gaaaccgccc gcgcggcagg cattgccatc
    56641 gtcactggcg atactaaagt ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc
    56701 gctggcatgg gcgcaattcc ggcgaatatt cactggggcg cacagacgct aaccgcaggc
    56761 gatgtattgc tggttagcgg tacactcggc gaccacgggg cgactatcct taacctgcgt
    56821 gagcagctgg ggctggatgg cgaactggtc agcgactgtg cggtactaac gccgcttatt
    56881 cagacgctgc gtgacattcc cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg
    56941 aacgcggtag ttcatgagtt cgcggcagcc tgcggttgcg gtattgaaat ttcagaagcg
    57001 gcactgccgg ttaaacctgc tgtgcgcggc gtttgcgaac tgctgggact ggacgccctt
    57061 aactttgcca acgaaggcaa actggtgatc gccgttgaac gcaacgcggc agagcaagtt
    57121 ttggcagcgt tacattccca tccactgggg aaagacgcgg cgctgattgg tgaagtggtg
    57181 gaacgtaaag gtgttcgtct tgccggtctg tatggcgtga aacgaaccct cgatttacca
    57241 cacgccgaac cgcttccgcg tatatgctaa taaaattcta aatctcctat agttagtcaa
    57301 tgaccttttg caccgctttg cggtgctttc ctggaagaac aaaatgtcat atacaccgat
    57361 gagtgatctc ggacaacaag ggttgttcga catcactcgg acactattgc agcagcccga
    57421 tctggcctcg ctgtgtgagg ctctttcgca actggtaaag cgttctgcgc tcgccgacaa
    57481 cgcggctatt gtgttgtggc aagcgcagac tcaacgtgcg tcttattacg catcgcgtga
    57541 aaaagacacc cccattaaat atgaagacga aactgttctg gcacacggtc cggtacgcag
    57601 cattttgtcg cgccctgata cgctgcattg cagttacgaa gaattttgtg aaacctggcc
    57661 gcagctggcc gcaggtgggc tataccccaa atttggtcac tattgcctga tgccactggc
    57721 ggcggaaggg catatttttg gtggctgtga atttattcgt tatgacgatc gcccctggag
    57781 cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc gtttctgtcg tcaccgaaca
    57841 aatccagagc cgcgtcgtca ataatgtcga ctatgagttg ttatgccggg agcgcgataa
    57901 cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc cgcctggata tggacgaact
    57961 ggtcagcgaa gtcgccaaag aaatccatta ctacttcgac attgacgata tcagcatcgt
    58021 cttacgcagc caccgtaaaa acaaactcaa catctactcc actcactatc ttgataaaca
    58081 gcatcccgcc cacgaacaga gcgaagtcga tgaagccgga accctcaccg aacgcgtgtt
    58141 caaaagcaaa gagatgctgc tgattaatct ccacgagcgg gacgatttag cccctatgaa
    58201 cgcatgttgt tcgacacctg gggcaaccag attcaaacct tgtgcctgtt accgctgatg
    58261 tctggcgaca ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc
    58321 actaccaatc tgaatttact gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac
    58381 gccctcgcct atcaggaaat ccatcgtctg aaagaacggc tggttgatga aaacctcgcc
    58441 ctgaccgagc agctcaacaa tgttgatagt gaatttggcg agattattgg ccgcagcgaa
    58501 gccatgtaca gcgtacttaa acaagttgaa atggtggcgc aaagcgacag taccgtgctg
    58561 attctcggtg aaactggtac aggtaaagag ctgattgccc gcgcgatcca taatctcagt
    58621 gggcgtaata atcgccgcat ggtaaaaatg aactgcgcgg caatgcctgc cggattgctg
    58681 gagagcgatc tgtttggtca tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt
    58741 ggtcgttttg aactggcgga taaaagctcc ctgttcctcg acgaagtggg cgatatgcca
    58801 ctggagttac agccgaagtt gctgcgtgta ttacaggaac aggagtttga acgcctcggc
    58861 agcaacaaaa tcattcagac ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa
    58921 aaaatggtcg ccgaccgtga gttccgtagc gatctctatt accgcctgaa tgtattcccg
    58981 atccacctgc cgccactacg cgagcgtccg gaagatattc cgctgctggc gaaagccttt
    59041 accttcaaaa ttgcccgtcg tctcgggcgc aatatcgaca gcattcctgc cgagacgctg
    59101 cgcaccttga gcaacatgga gtggccgggt aacgtacgcg aactggaaaa cgtcattgag
    59161 cgcgcggtat tgctaacacg cggcaacgtg ctgcagctgt cactgccaga tattgcttta
    59221 ccggagcctg aaacgccgcc tgccgcaacg gttgtcgccc aggagggcga agatgaatat
    59281 cagttgattg ttcgcgtgct gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct
    59341 gcgcaacgtc tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg actgggaatt
    59401 gataaatcgg cattgattta acagatgtgg ttatactcga tcaacgttca atgaaaggag
    59461 gcagtatggc agtttcagca agaaatcaat taaccggaac agttagcgca gtagcaatgg
    59521 gcgctgtgaa tgatgaagta gaactgacgc tggcaggtgg tgcaaagctg gtggcaattg
    59581 tgactcacag cagccagcaa gcgctgggtc tggcgaaagg gaaagaagcg attgccctga
    59641 ttaaagcccc gtgggtaacg ctggcaacgg aagactgtgg actgaaattc tctgctcgca
    59701 accagtttgc tggcagcgtg tcgacaatca ccgaaggcgc agtgaacgcc actgtccata
    59761 tcaaaacgga tgcgggtttt gaaattgtgg cagtggtaac aaatgaaagt caggatgaaa
    59821 tgaaactcac cactggctcg cgcgttattg cattgatcaa agcatcagcc atcctgattg
    59881 ccaccaaagc gtaagcgcct tatttgccgg agagtatggc tctccggcag atttatttct
    59941 ttggacgata tttttctggc aactccggca ccggacgctt gtcatcgatg agatgacgca
    60001 cggttaagat cggatgacgc cacagcattc gcggcccagc ccaacgcata atctgtttca
    60061 tctcttcacg ctttgcaggc tggtaacagt gcaccggaca ctgcttacag gctggtttct
    60121 cttcgccgaa cacacattta tccagccgct tttgcgcgta agtaaacaac gtctcataat
    60181 gctccggctc cgctgacgcc tgcgggcatt tcgcttgata aagatcgatc atttttttaa
    60241 tcgtcagttt ttcacgagag atacgcttgc cggacatgct gcctccatct cattaagatg
    60301 catttatatt acaacttaat cttaaagggc actatgactc taaagaagaa gggttagcca
    60361 accgatacaa ttttgcgtac ttgcttcata agcatcacgc aaaagctgca aaacagcatc
    60421 tttcccggaa ccagcatcaa gaactcgccg ttcgcttctt cccctgaaat gattaactcc
    60481 ggtatcatgt gcgccttatg tgattacaac gaaaataaaa accatcacac cccatttaat
    60541 atcagggaac cggacataac cccatgagtg caatagaaaa tttcgatgcc catacgccca
    60601 tgatgcagca gtatctcaag ctgaaagccc agcatcccga gatcctgctg ttctatcgga
    60661 tgggtgactt ttacgaactg ttttatgacg acgcgaaacg cgcctcgcag ctgctggata
    60721 tttcactgac caaacgcggt gcttccgcgg gagagccgat cccgatggcg gggatcccct
    60781 accatgcggt ggaaaactac ctcgccaaac tggtgaatca gggcgagtcc gttgccatct
    60841 gcgaacaaat tggcgatccg gcgaccagca aaggtccggt tgagcgcaaa gttgtgcgta
    60901 tcgttacgcc aggcaccatc agcgatgaag ccctgttgca ggagcgtcag gacaacctgc
    60961 tggcggctat ctggcaggac agcaaaggtt tcggctacgc gacgctggat atcagttccg
    61021 gtcgttttcg cctgagcgaa ccggctgacc gcgaaacgat ggcggcagaa ctgcaacgca
    61081 ctaatccggc ggaactgctg tatgcagaag attttgctga aatgtcgtta attgaaggcc
    61141 gtcgcggcct gcgccgtcgc ccgctgtggg agtttgaaat cgacaccgcg cgccagcagt
    61201 tgaatctgca atttggcacc cgcgatctgg tcggttttgg cgtcgagaac gcgccgcgcg
    61261 gactttgtgc tgccggttgt ctgttgcagt atgcgaaaga tacccaacgt acgaccctgc
    61321 cgcatattcg ttctatcacc atggaacgtg agcaggacag catcattatg gatgccgcga
    61381 cacgtcgtaa cctggaaatt actcagaacc tggcgggcgg tgcggaaaac acgctggctt
    61441 cagtgctcga ctgcactgta acgccaatgg gtagtcgtat gctgaaacgc tggctgcata
    61501 tgccagtgcg cgatacccgc gtgttgcttg agcgccagca aactattggc gcattgcagg
    61561 atttcaccgc cgagctacag ccggtgctgc gtcaggtcgg cgacctggaa cgtattctgg
    61621 cacgtctggc tttacgaact gctcgcccac gcgatctggc ccgtatgcgt cacgctttcc
    61681 agcaactgcc ggagctgcgt gcgcagttag aaaatgtcga tagcgctccg gtacaggcgc
    61741 tgcgtgagaa gatgggtgag tttgccgaac tgcgcgatct gctggagcgg gccattatcg
    61801 acacaccgcc ggtactggtg cgcgacggtg gtgttatcgc atcgggctat aacgaagagc
    61861 tggatgagtg gcgcgcgctg gctgacggcg cgaccgatta tctggagcgt ctggaagtcc
    61921 gcgagcgtga acgtaccggc ctggacacgc tgaaagtcgg atttaacgcg gtgcatggtt
    61981 attacattca gatcagccgt ggacaaagcc atctggcacc gattaactac atgcgccgcc
    62041 agacgctgaa aaacgccgaa cgctacatta ttccagagct aaaagagtac gaagacaaag
    62101 ttctcacttc aaaaggcaaa gcactggcac tggaaaaaca gctttatgaa gagttgttcg
    62161 acctgctgtt gccgcatctg gaagcgttgc aacagagcgc gagcgcgctg gcggaactcg
    62221 acgtgctggt gaacctggca gaacgggcct atacgctgaa ctacacctgc ccgaccttta
    62281 ttgataaacc gggtattcgc atcaccgaag gccgccatcc ggtggttgaa caggtgctga
    62341 atgagccgtt tatcgccaac ccgctgaacc tgtcgccgca gcgtcgcatg ttgatcatta
    62401 ccggtccgaa tatgggcggt aaaagtacct atatgcgcca gaccgcactg attgcgctga
    62461 tggcctacat cggcagctat gtaccggcac aaaaagtcga gattggacct atcgatcgca
    62521 tctttacccg cgtaggggca gcggatgatc tggcatccgg gcgttcaacc tttatggtgg
    62581 aaatgaccga aaccgccaat attttacata acgccaccga gtacagtctg gtgctgatgg
    62641 atgagattgg gcgcgggacg tccacttacg atggtctgtc gctggcgtgg gcgtgcgcgg
    62701 aaaatctggc gaataagatt aaagcgttga cactgtttgc cacccactat ttcgagctga
    62761 cccagttacc ggagaaaatg gaaggcgtcg ccaacgtgca tctcgatgcg ctggagcacg
    62821 gcgacaccat tgcctttatg cacagcgtgc aggatggcgc ggcgagcaaa agctacggcc
    62881 tggcggttgc agctctggcc ggcgtgccaa aagaggttat taagcgcgca cggcaaaaac
    62941 tgcgtgagtt ggaaagcatt tcgccgaacg ccgccgctac gcaagtggat ggtacgcaaa
    63001 tgtctttgct gtcagtgcca gaagaaacgt cgcctgcggt cgaagcactg gaaaatctcg
    63061 acccggattc actgactccg cgtcaggcgc tggagtggat ttatcgcttg aagagtctgg
    63121 tgtaataaca attcccgata gtcttttgct atcgggaata ttaacgacaa cagacgaatc
    63181 aaataaaaac accctgtata ataggaaatc ttattttaca gggtaaaacc atgccatcta
    63241 cacgctatca aaaaattaat gcccatcact atcgttatat atgggccgtt ggtgatattc
    63301 atggtgacta tcaattatta caatcccgct tacatcaact ctcttttttc cctgaaaccg
    63361 acttacttat ttctgtcggc gataatattg atcgtgggcc ggagagtctt aacgtactac
    63421 gcctgttaaa ccagccctgg tttacatcgg ttaaaggcaa ccatgaagcg atggcgcttg
    63481 atgcattcga aactggcgat ggaaatatgt ggcttgccag cggcggcgac tggttctttg
    63541 aattaaatga gacggaacaa caagaagcaa taaatctgtt gctgaaattt catcgccttc
    63601 cacatattat tgaaatcact aacgacataa taaaatatgt catcgcacat gcagattatc
    63661 cggggaatga atatctcttt ggcaaagaaa tagcggagag agaactactc tggcctgttg
    63721 atcgtgtgca gaaatcgctt aatggcgagt tacaacaaat aaacggcgct gattatttta
    63781 tatttggaca tatgatgttt gataacattc agacgttcgc aaaccagatt tatattgata
    63841 ccggatcgcc gaaaagcggg cggctgtcat tttataaaat aaagtagtct catgcttctt
    63901 ctgtgaagca tgagtaaccc ggtgttattg caggccatta ttcatttttc gctaccagca
    63961 aagagagatc ctgcttcgcc agcgcgcgac tggcactctc cggcaaaccg tcgtctgtaa
    64021 taatctgatc aaactcgctt aatggtaacg ccagccatgt cgccacctga ccatatttcg
    64081 tcgcatcaca gaccaaaact cgctggcggc tggcactggc aatcgcccgt ttcaccgtga
    64141 ctttatcttc cgctggcgta gaaatccccc gcacactcca tgacgatgca gaaataaaag
    64201 cctgatcaat catcaggctg cgcagcatgg tcgcagcggc ttccccgaca caggaacggt
    64261 tttcccgaca cactgcaccg ccagtgtgaa taattgtgca attactgttg tcgagcaagt
    64321 agtccgcaat aacgaaatcg tttgtgacca cagtcagtga ctccatgtga atcagatgct
    64381 gtgctatcgc tagcgtggtc gttcccgcat ccagatagat acaacttccc ggctgaacaa
    64441 gacttgccgc cagcttgcca atagccgctt tttgcgtcat tgccagcgca gtttttacct
    64501 gatgagaagg ttcatgcgcc acgcgtcccg gagactggac gcctccggac accagcacaa
    64561 cggctccctg ctgctccagt ttttgtaaat cccgacgaat ggtcatatgt gacacattca
    64621 ttctgtccgt tagttcagca atactgacaa tgcctttttc agctaccatc tcaaggatga
    64681 tttggcgacg ctctacgggt atcaactttt gctccttcct ttgtcctgct gacattctac
    64741 gctatttgcc tgcgaaacgt gcgcggcgca actaacgctt agttcacata aaataacaca
    64801 caatgttaat ttttgtgaat cagctcacca tatcgttatc ttccagcgcc tatattcaca
    64861 atatcaaaca aaatatcact taaattaaca aggagagcag atgaaaacgg gatctgagtt
    64921 tcatgtcggt atcgttggct tagggtcaat gggaatggga gcagcactgt catgtgtccg
    64981 cgcaggtctt tctacctggg gcgcagacct gaacagcaat gcctgcgcta cgttgaaaga
    65041 ggcaggtgct tgcggggttt ctgataacgc cgcgacgttt gccgaaaaac tggacgctct
    65101 gctggtgctg gtggtcaatg cggcccaggt taaacaggtg ctgtttggtg aaacaggcgt
    65161 tgcacaacat ctgaaacccg gtacggcagt aatggtttct tccactatcg ctagtgctga
    65221 tgcgcaagaa attgctaccg ctctggctgg attcgatctg gaaatgctgg atgcgccagt
    65281 ttctggtggt gcagtaaaag ccgctaacgg tgaaatgact gtcatggcct ccggtagcga
    65341 tattgccttt gaacgactgg cacccgtgct ggaagccgtt gccggaaaag tttatcgcat
    65401 aggtgcagaa ccgggactag gttcgaccgt aaaaattatt caccagttgt tagcgggcgt
    65461 acatattgct gccggagccg aagcgatggc acttgcagcc cgtgcgggga tcccgctgga
    65521 tgtgatgtat gacgtcgtga ccaatgccgc cggaaattcc tggatgttcg aaaaccggat
    65581 gcgtcatgtg gtggatggcg attacacccc gcattcagcc gtcgatattt ttgttaagga
    65641 tcttggtctg gttgccgata cagccaaagc cctgcacttc ccgctgccat tggcctcaac
    65701 agcattgaat atgttcacca gcgccagtaa cgcgggttac gggaaagaag acgatagcgc
    65761 agttatcaag attttctctg gcatcactct accgggagcg aaatcatgat caagattggc
    65821 gttatcgccg atgattttac cggcgcgacg gatatcgcca gttttctggt ggaaaacggt
    65881 ctaccaacgg tacaaattaa cggtgttcca acaggtaaaa tgccggaagc aatcgacgca
    65941 ctggtgatca gcctgaaaac gcgttcctgt ccggtggttg aagccacaca gcaatcgctg
    66001 gcggcactga gctggttgca acagcaaggt tgcaaacaga tctatttcaa atactgctct
    66061 actttcgaca gttcggcgaa aggtaatatc ggcccggtta ccgatgcatt aatggatgct
    66121 ctcgacacgc cgtttacggt cttctctccg gccctgccgg tcaacggacg tacggtttat
    66181 caggggtatt tgttcgtaat gaatcaactg ctggccgaat ccgggatgcg ccatcacccg
    66241 gtaaatccca tgaccgacag ctatcttccc cgtctggttg aagcgcaatc cacagggcgc
    66301 tgcggcgtcg tttcggcaca tgttttcgaa caaggtgtgg atgccgttcg tcaagagctg
    66361 gctcgcttac agcaagaggg ctaccgctac gcggtgcttg atgcgctgac cgaacaccat
    66421 ctggaaattc agggagaagc cttgcgcgat accccactgg taacgggcgg ttctggtctg
    66481 gcgattggcc ttgcccggca gtgggcgcaa gaaaacggta accaggctcg caaagcaggg
    66541 catccgctcg ctgggcgcgg cgtagtgctc tccggttcat gctctcaaat gaccaaccgc
    66601 caggtagcac attaccgtca aattgcacca gcccgtgaag ttgatgtggc acgctgcctc
    66661 tcaattgaaa ctctggccgc ttatgcacac gaactggcag agtgggttct gggccaggaa
    66721 agtgtacttg ctccactggt ttttgccacc gccagcactg acgcattggc agcaattcaa
    66781 cagcaatacg gtgcacaaaa agccagtcag gcagtagaaa cactgttttc tcaactagcg
    66841 gcgcggttag cagcggaagg cgtgacacgc tttattgtcg caggcggtga gacctccggc
    66901 gtagtcacac agagcctggg aataaaaggg tttcatattg gcccaaccat ttctcccggc
    66961 gtgccgtggg taaacgcact ggataaacct gtctcactcg cccttaaatc tggcaacttc
    67021 ggtgatgaag cctttttttc acgagcccaa agagagtttt tatcatgagc gatttcgcaa
    67081 aagtagagca gtctttgcga gaggagatga cgcggattgc cagttcattc tttcagcgcg
    67141 gctatgcaac cggttcggct ggcaatctgt cgctgctttt acctgacggg aatttactgg
    67201 cgacaccgac aggttcatgc ctgggcaatc tcgatccgca gcggctttcc aaagtcgccg
    67261 cggatggcga atggttaagt ggtgacaaac cctcgaaaga ggtgctcttt catctggcgc
    67321 tgtatcgcaa caatccgcgc tgtaaagcgg tggtgcattt gcacagcaca tggtcgacgg
    67381 cgctttcctg cctgcaaggg ctggacagca gcaacgttat tcgtccgttc acaccatacg
    67441 tggtgatgcg gatgggaaat gtcccgctgg tgccttatta ccgaccgggc gataaacgca
    67501 tcgcacagga tctggcggaa ctggcagcag acaatcaggc ttttttactg gcaaatcatg
    67561 gcccagtggt ttgcggtgaa agcctgcaag aagccgccaa caatatggaa gagctggagg
    67621 aaacggcaaa gctgattttt attctcggtg accgcccgat ccgttatctg accgcaggtg
    67681 aaattgcgga attaaggagt taatgcaatg cctcgttttg cagctaattt atccatgatg
    67741 ttcaccgaag tgccttttat tgaacgcttc gccgcagcgc gaaaagccgg gttcgatgct
    67801 gtggaatttc tgtttcccta tgactactcc accctgcaaa tccaaaagca actggagcaa
    67861 aaccatctga cactggcgct gttcaatacc gctcccggag atattaatgc gggggaatgg
    67921 ggattatccg cccttcccgg acgtgagcac gaagcacacg cagacatcga cctggcactg
    67981 gaatatgcat tggcgctcaa ttgtgaacaa gtccatgtga tggcaggcgt cgtgcccgct
    68041 ggcgaagatg ctgaacggta cagggcagta tttatcgata atctccgcta cgctgccgac
    68101 cgctttgcgc cacacggcaa gcgaatatta gttgaagcac tcagccccgg cgttaagcct
    68161 cattatctct tctccagcca gtatcaggca ctggctattg ttgaagaagt tgcacgagat
    68221 aacgtgttta ttcaactaga tacttttcat gcgcaaaaag tagatggcaa cctgacgcat
    68281 ttaatccgcg actacgccgg gaaatatgcg cacgtacaaa ttgccggact acctgatcga
    68341 catgaaccgg acgatggaga aatcaactat ccgtggctgt tccgcctgtt cgatgaggtg
    68401 ggatatcagg gctggatcgg ttgtgaatat aaacctcgtg gcctcaccga agaagggctt
    68461 ggctggtttg acgcctggcg ctaattcgtt aaatccctaa ttacaaagta cccatacatc
    68521 cccccatcta cggatggggt aggttaacta taactttcag acagggtttc ccatgtccac
    68581 aattacattg ttatgcattg cgttaactgg cgtaatcatg ctgttgctgc tggtcatcaa
    68641 ggcaaaggta caaccattcg ttgctctgct cctcgtcagc ctgttagtcg cacttgcggc
    68701 gggtataccg gcgggcgaag tgggtaaagt gatgatcgcc gggatgggcg gtgtgcttgg
    68761 ttccgtcact attattattg gtctgggcgc tatgctgggg cgtatgatcg aacactctgg
    68821 tggtgcagag tcactggcta attatttcag tcgcaagtta ggtgacaaac gaactatcgc
    68881 tgcgctgact ctggcagcgt tcttcctcgg tattcccgtc ttctttgatg tcggctttat
    68941 tattcttgcg ccaatcattt acggttttgc caaggttgcc aaaatatcgc cactcaaatt
    69001 tggcctgcct gtcgctggaa tcatgctaac tgttcatgtg gcggtaccgc cgcatccagg
    69061 ccctgtcgcc gcagcggggt tactccacgc agacatcggc tggctaacca tcatcggtat
    69121 tgcgatttct attcccgtag ggattgttgg ctactttgca gcgaaaataa tcaataagcg
    69181 ccaatatgcg atgtcggtgg aagtactgga acagatgcaa ctggctccgg ccagtgagga
    69241 aggcgcgaca aaattaagcg ataaaataaa tccaccgggc gtcgcgctgg tcacctcgct
    69301 aattgttatt cctatcgcga ttatcatggc gggtacggtt tccgcaacac tgatgccgcc
    69361 ttcgcatccc ctgcttggta cgctacagct gatcggctca ccaatggtag ctctaatgat
    69421 tgcgctggtg ctggcattct ggttattggc tttacgtcgc ggctggagct tacaacacac
    69481 cagcgacatt atgggctcag cccttcccac cgcggcggta gtgattttgg ttactggtgc
    69541 tggaggggta tttggcaaag tgctggtgga atcgggcgtc ggcaaagccc ttgccaacat
    69601 gctacaaatg attgacctgc ctctgttacc agccgcattt attatttcac tggcgctgcg
    69661 tgcatcgcag gggtcagcaa ccgtagcaat cctgacaacc ggcgggttac tctcagaagc
    69721 ggtgatggga ttgaatccga ttcagtgcgt attggtgacg ctggcagcct gctttggtgg
    69781 gcttggcgcg tcacatatta atgactcagg gttctggatt gtgaccaaat atctggggtt
    69841 atcggtagca gacggtctga aaacctggac agtgttaacg accattctcg gttttaccgg
    69901 attcttaatt acctggtgcg tatgggcggt aatttgacct taataaaaag gtccgatggg
    69961 catcggacct tttatttagc gcttaccttc cgccagcagc ggcgggatgc tcggcaccat
    70021 cggcgcgtca tcaatatctt tctgtgtcat gcggaacgct tcgggataat gttcgcggct
    70081 ggtgcggcgt agcggttcgg tatcgcgcca ggtatagagg caatgctggc actggtagac
    70141 cgtccagaca tcttttaccg gcgatttcgc catcacttca atctgttcat cagcacaacg
    70201 tggacaaatc atcttgttct ccttatttac gtgcggccag catagcggtc agtttttcag
    70261 cccaggcttt ggtttccggt aaatccacca ccggctggct gtagtgacca cggttgtccg
    70321 gggcgacagg cgtggtggcg tcgataatca gcttgtcggt gataccggcc gggcttgagc
    70381 cggggtcgag ttccagcacg gacatattcg gcaactgcac caaatcccct gccgggttca
    70441 ctttcgaaga gagcgcccac atcacctgcg gcaggttgaa cggatcaacg tcttcatcga
    70501 ccataatcac catcttcacg tagcccagac cgtgtggcgt ggtcatcgca cgcaggccca
    70561 ccgcgcgggc aaagccaccg tagcgttttt tggtggagat aatcgccagc aggccgtggg
    70621 tgtacatggc atttaccgcc tgcacttccg ggaactcggc tttcagttgc tgatacagcg
    70681 gcacgcaggt ggctggcccc atcaggtagt cgatttccgt ccacggcatc ccgagataga
    70741 gcgattcaaa aatgggtttg ctgcgataag agactttatc gatacgcacc acggtcatgt
    70801 tacgcccgcc ggagtagtgc ccggtaaact caccaaacgg cccttcgatt tcgcgtttac
    70861 ggctttcgat gaccccttcg aggatcactt ctgaacccca cggcacatca aaaccagtca
    70921 gcggggcggt ggcgatcggg tacgggcttt cacgcagcgc gcctgccatt tcgtactcgg
    70981 actgatcata tttcagcggc gtggccccca tcagggtgat gatcggatcg ttaccgagtg
    71041 tgatggcaat cggcagatct tcaccgcgtt cttccgcttt atgcagatgc agggcgatat
    71101 cgtgcatcgg caccggttgc agaccgagct tacgcttacc cttcacttcc atacggtaga
    71161 tgccgacgtt ctgcttgccg aagttatccg gatcaagcgg atcgcgggaa accacgcacg
    71221 ctttgtcgag atagaaaccg ccatcaccgt cgtttaaacg aaacagaggc agaatatcga
    71281 acaggttgat ctcgtcgcca tcgacggtgt tctgcgccca ggctggattg gcgcggcgct
    71341 ccggggcaat ggggaagtta tcccagcggc ggataaactc atcaatctgt tttttaaccg
    71401 gggtgtttgg cggcaggccg agggaaatcg cgtggttctg ccaggaaccg atggtgttca
    71461 ttgccacgcg ggcatcgata aagccgcgaa tattgtcaaa ccacagcgcc ggtgcaacat
    71521 cgccgatacg tccggtggcg ttggcagcgg ctgccagatc cggttccgcg ttcacctctt
    71581 cgctgatttt cagtaactgg ccgtggtcat caagcgcctg taaaaagctg cgtaaatcat
    71641 caaatgccat tattcattct cctgagaaaa attccgggcc tgcggcaatc cttgccagcg
    71701 cctggcgtga ggatgttcga ggccaaattg atccagcacg cgggctacca cgtggtggac
    71761 aatgtcatct accgtttcgg gatggttata aaaggcaggc atcggcggca ccatcgccac
    71821 gcccatgcgt gaaagtgcga gcatattttc gagatggatg gtgctaagcg gcatttcacg
    71881 cggcaccagc actagtttgc ggccttcttt gagcacgacg tccgccgcgc gccctaccag
    71941 gccatcggcg taaccggcac ggataccggc gagcgttttc atactgcacg gaataacgat
    72001 catgccgtcg gtacgaaagg aacctgagga gatggttgcg gcctgatccg ccgggttatg
    72061 gcagaagtct gcgagggcag caacatcgcg ggcgttgtaa ggcgtttcca gttcaatggt
    72121 ggttttcgcc cacttcgaca tcaccagatg agtctcgaca ttcggcatct cccgcagcgc
    72181 ttgcagtaat gccacaccaa gaggcgctcc ggtggcccct gtcatcccga cgatcagtct
    72241 cattgtttat tctcttaatt gttcgtatac gaacgttatt attaaaatac gcctctgttt
    72301 tcatcgcggc aagcactgaa aagtaacaac atcttcttcg tcactgaaaa accgctatga
    72361 tagcggcaga tagtttgccc ggagtttaca tggcgttacg aaataaagcg ttccatcagt
    72421 tacgacagct ttttcagcag cacacggctc gctggcagca cgagttacct gacctcacca
    72481 aaccacagta cgcggtgatg cgcgccattg ctgataagcc tggtatcgaa caggtggcac
    72541 tgatagaagc agcagtcagc accaaagcaa cgctggcaga aatgttggca agaatggaaa
    72601 atcgtggctt agtcagacgt gaacatgacg ccgctgacaa gcggcgacgc tttgtctggc
    72661 tgactgccga aggagaaaag gtacttgctg cggcgatccc cattggtgac agcgtggatg
    72721 aggaattttt ggggcgtttg agtgcagaag agcaggagct gtttatgcag ttggtgcgca
    72781 agatgatgaa cacatagggt gcaaattgcc ggatgcgacg ctatcgcggc ttatccagcc
    72841 cacaaaaaca catgcttccc aaacaaaaga aaaaaggcca gcctcgcttg agactggcct
    72901 ttctgacaga tgcttactta ctcgcggaac agcgcttcga tattcagccc ctgcgtttgc
    72961 aggatttcgc gcaaacggcg caggccttca acctgaatct ggcgaacacg ttcacgggtg
    73021 aggccaattt cacgacctac atcttccagt gttgccgctt cgtaccccag caaaccgaat
    73081 cgacgtgcca gtacttcacg ctgtttggcg ttcagctcga acagccattt gacgatgctc
    73141 tgcttcatat cgtcatcttg cgtggtatct tccggaccgt tctctttttc atcggccagg
    73201 atgtccagca acgctttttc ggaatcacca cccagcgggg tgtctaccga ggtaatgcgc
    73261 tcgttaagac gaagcatacg gctgacgtca tcaactggct tatccagttg ctctgcgatc
    73321 tcttccgcac ttggttcgtg gtccagttta tgggacaact cgcgtgcggt tcgcaggtaa
    73381 acgttcagct cctttacgat gtgaatcggc aaacgaatag tacgggtttg gttcataatc
    73441 gcccgttcga tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg gaaaccacgt
    73501 tccgggtcaa acttctctac cgcgcggatc agccccaggt tgccctcttc gatcaggtcc
    73561 agcaacgcca gaccacgatt gccataacgg cgggcaattt ttaccaccag acgcaagtta
    73621 ctctcgatca tccggcggcg ggaggcaaca tctccacgca gtgcgcgacg cgcaaaataa
    73681 acttcttctt cggccgttaa cagtggtgaa taaccaatct caccaaggta aagctgagtc
    73741 gcgtccaaca cacgctgtgt ggctccctgc gataacagtt cctcttcggc caaatcgtta
    73801 tcactgggtt cctcttctac taaggccttt tcgtcaaaaa cctcaactcc gttctcatca
    73861 aattccgcat cttcatttaa atcatgaact ttcagcgtat tctgactcat aaggtggctc
    73921 ctacccgtga tcccttgacg gaacattcaa gcaaaagcct ggttccgccg atttatcgct
    73981 gcggcaaata acgcagcggg tttacggatt tccccttgta acgaatttca aaatgcaagc
    74041 gtgttgaact ggttccggtg ctacccatgg ttgctatttt ttgccccgcc ttcacttctt
    74101 gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa tcatcattat
    74161 gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt accggcataa acaacgcggc
    74221 catctgcggt cgcgataatt gcctgtcctt tgctgcccgc gatatcaatc cccttgttgc
    74281 ccccctcaga agcgccaaag gtttcgatca ctttgccctc agtcggccag cgccaggtgg
    74341 agataggcgt actggttgat gtactgctga cagtcggctc ggttgtgctt gctgttggta
    74401 ccgttacagg cgctgtgacc gtggtcgcag ttggcttgtt gttcggcaac attttgttag
    74461 cactctgttc acccgaagac tcagaatacg taattgtcgg ttgcgaagca acagcaacgg
    74521 tggaattttg tgcaggcttg atcacaactc cttgctctgc tgcatcggcc tgggtaatgg
    74581 catttccgcc agtgattggc gtaccggaag cattacccac ctgtaaggtc tgaccgacgt
    74641 tcagcgcgta tggtgcctga atattgttgc gctgagcaag gtcacggaaa tcgttgccag
    74701 taatccaggc gatatagaaa agtgtgtcgc cttttttcac ggtataggta ctgccgctat
    74761 aactgccttt cggaatgttc ccatactgac ggttgtagac gatgcgtccg ttttccatct
    74821 gtaccggctg ctgagctact ggctgtactg gctggatttg cggttgttga gtagcctgaa
    74881 tttgtggctg ctgtaccggc tgaatttgcg gttgctgcgc tgtagacgtc gttcccattt
    74941 tcggcggcgg cgtaatcaac ataccagaat tggtatttgc aggcgcattg ccattaacgg
    75001 agctgaccgg tgccggtgga tttgaagtgt cagaacagcc tgccagccat agcgaaacca
    75061 gtgacaaagc cgcaatgcgg cgaacggtga attttgggct tcccgcgctc atttatcccc
    75121 caggaaaaat tggttaataa ccagtgacat aattaccgtg caaggcacca tactgaacac
    75181 tggaaaagat gttcacgata cgctgacctg cggcaaaata accaggaaaa atccaggtat
    75241 ttcctcacgt tttaagccag ctcacccttc actaaaggga caaagcgcac ggcctccacg
    75301 gtatcgataa taaattcgcc tccccgacga cgcacccgtt tcaaatactg gtgctcctcc
    75361 cctacgggta agacgagaat cccgccttcg tccagctgcg tcattagtgc agttggaatt
    75421 tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg cacgtgcctg ccaaccttgc
    75481 catccatcgc catgacgggt tgaaacatta tgtaaatcaa gatttttcag gcggcgacgc
    75541 gcctgccact gcaaaccttt aatccgttcg accgagcaaa catgctggac aagatgcgcc
    75601 aggattgccg tttgatatcc cgaaccggtg ccaatttcca gcacccgcga ctgcggcgtc
    75661 agctcgagta attcggtcat tcgcgccacc atatacggct gcgaaattgt ctgcccctga
    75721 cctatcggca aggcgatatt gtcccaggct ttttgttcaa acgcttcatc aacgaatttt
    75781 tcacgcggca cggcggcaag tgcattcagc acctgctcat cctgaatacc ttgcgcacgt
    75841 aattgatcca gaagtgcttg tacgcgtctg cttaccattg cgtgccaact cccacgctgt
    75901 ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca tgcagcggcg
    75961 tgatggagac atagccctca tctaccgcag caaaatcggt ccccggaccg gcatcacatt
    76021 taccgcccgg cgggccaatc cagtacagcg tattgccgcg cggatcttgc tgcgggatca
    76081 cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg atttgatcca
    76141 atggtaaatc cggaacgtta atattaagaa tacgtccggt gcgcagcggc tctttacaca
    76201 gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc gtaatgttta tgcccgtcaa
    76261 gcgagacggc aagcgccgga aaacctaaat gacggccttc catcgcggcg gctaccgtac
    76321 cggaataaat aacatcatcc cccagattcg gcccggcgtt aattccggac acaacaatgt
    76381 ccgggcgcgg acgcatcaga gcattcacgc caagatagac gcaatcggtc ggggttccca
    76441 tttgcacagc aatatcacca ttttcagagg taaacgtgcg cagggaggat tccagtgtca
    76501 gagaatttga agcgccgctg cggttacgat cgggggcgac cacctgaacg tcagcaaact
    76561 cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg taccccgtca tcattactca
    76621 gcaatatgcg cataatcacc tgttgtgttg ataagttccc tgacaacgct ggttgcaaaa
    76681 ctacccgccg gaagccagaa acggatttct acggtgacgt catcccacca gttccagctt
    76741 aattgttgcg gatacagcag catcgctctg cgcgcggctt caactttttc gcgcaccagt
    76801 aaggcttgta attcagtttc tgcggcgaca gctgcttgtt cgaatgccaa cgcttcacgc
    76861 tgagttcccc attcgccact gcccggcaat gcggcggtta tcatcagctc tttatcgttg
    76921 acgcgacgct gtaattccgc cagttcttcg gtggttgcga caaaccagct accacgtccg
    76981 gctaattgta gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt gaggcgctca
    77041 gcaacaatct gattaaacaa cgcactgcgg gctgccgaca accaaaaact ccgtttattg
    77101 cgatcgcgca ccggagtatt ggtttgcgcc cagcgcagcg cgccctgcaa gttgctaccg
    77161 ccaatcccaa aacgttgggc accaaaatag ttaggcacgc cttttacgca aatatcgatc
    77221 agacgttgtt caacgtcatc gcgattgctc acttcgcgca gaaccagggt aaaggcgtta
    77281 cctttcagcg cgcctaaacg cagcttgcgc ttgtgccgcg catactccag cacctggcag
    77341 ccttccagtt gaaaggcgct cagatcgggc atttccttgc ccggcacgcg agcacataac
    77401 cactgttccg ttacagcatg tttgtctttt tgcccagcga agctgacttc acgggcatga
    77461 attttaagga atttcgccag tgcatccgcc acaaaacggg tattgcagcc gtttttgagg
    77521 attctaacca gaatatgctc accttcacca tcaggctcaa agcccaaatc ttccaccacc
    77581 acaaagtctt ccggattggc tttcagcagc ccggtgcctt gcggtttacc gtggaggtaa
    77641 gtgagattat caaactcaat cattttgttg ccttaatgag tagcgccacc gcttcacagg
    77701 caatcccttc cccacgtccg gtaaatccaa gtttttccgt agtagtggct ttcacgttaa
    77761 catcatccat atggcagccg agatcttcgg caataaacac gcgcatttgt ggaatgtgcg
    77821 gcaacatctt cggtgcctga gcgatgatag tgacatcgac gttgcccagg gtataaccct
    77881 tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg gctgtcggca cctttaaatg
    77941 ccggatcggt atccgggaac agtttgccga tatcccccag cgccgccgcg ccaagcaatg
    78001 catcggtcaa cgcatggagc gccacgtcgc catcagaatg cgccagcaat cctttttcgt
    78061 aaggaatgcg tacgccacca atgataattg ggccttcacc gccaaaggcg tgtacgtcaa
    78121 aaccgtgtcc aattcgcatt atgtattctc ctgatggatg gttcgggtga ggtaaaactc
    78181 ggccagtgcc aaatcttccg ggcgcgtgac tttaatgtta tccgcgcggc cttcgaccaa
    78241 ctgcggatga aatccgcaat attccagcgc cgaggcttcg tcggtaatgg tagcgccttc
    78301 atttagagcg cgcgtcaggc agtcatgtaa cagctcacga gggaaaaatt gcggcgtcag
    78361 cgcgtgccat aagccgttgc gatcaacggt atgagcaatg gcatttttgc ccggttcggc
    78421 acgtttcatc gtatcgcgca ctggtgcggc gaggatcccc cccgtgcggc tggtttcgct
    78481 caacgccaac aatcgcgcga ggtcatcctg atgcagacaa ggacgagctg cgtcatgcac
    78541 caatacccac tgcgcgtcgc cagcggcttt cagacctgcc agcaccgaat cggcacgctc
    78601 atcaccgcca tctacaacgg tgatttgcgg atgattcgcc agaggaagtt gtgcaaaacg
    78661 gctatcgcca ggacttatgg caatgacgac atgtttcacc cggggatgcg ccagcagcgc
    78721 atgcaccgag tgttcaagaa tggtttgatt accgattgag agatattgct taggacattc
    78781 cgtttgcatt cgacggccaa atccggccgc cggaaccacg gcgcaaacat ccaaatgagt
    78841 ggttgccatg ttaattcctg ggctgattta tcgattgttt tgccccgcag actgtgcgcg
    78901 cttcgacgcg tcaggcacca gacgataaaa agtttcgccc ggcctggtca tgctgagttc
    78961 attacgcgca cgctcttcga gcgcctcctg gccgccattg agatcgtcaa tttcggcaaa
    79021 aagttgatcg tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg ccacatcatc
    79081 attgacgcgg gtatagtcat gtataccgtt cttaccgaac cacagcgaat actgtagcca
    79141 gaccagaata gccagcaaca gcagcgttag tttacccatc ctgccccctg aaaaacggca
    79201 tcatcatccc atgcatccga agacgactct acatcctctg ttggggatac cgcgacaacg
    79261 cgggcaaatg taccacattt gtccattgtt acgtataccc agggcgtgca gaacataatc
    79321 tcattattag ttacggtttg aattatgaac agaggagaca agaaagtaca aattagccca
    79381 gtagccacat aaacagtgcg ccaaacataa tgcctactgt caccagggtg aaaacaatac
    79441 tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat ccccaccact accgcgacag
    79501 gcatcagtgc cagaaagaaa ggccaggtgt agataaagaa gaacagcgtg ttagagccat
    79561 aaatcaacat cggcatcgcc agcgcaaata accatgagat aaaaccgatc acggcaccag
    79621 gcagtgacca tgtggtttct tcatcctcag taaggctgtc gttatttgtt agtgtaatgt
    79681 tatggctatt acgcatattt gatcctgtta ctttgacgaa ccgggcatgg aaacccggtg
    79741 gtgtctcagg atctgataat atcgttctgt ctcaacagat ctaataattg ctgtaccaaa
    79801 tttgttacta attgttcacc attgagatga atttctgccg attcaggcgc ttcgtaaacg
    79861 gaatctattc ccgtaaagtt acgcagttca ccggcacgcg ctttcttata taagcctttt
    79921 ggatcgcggg cttcgcaaat cgccagcggt gtatcgacaa acacttcgat aaagcgccct
    79981 tctcctacgc gttcgcgaac catctggcgt tcggcgcggt gtggcgagat aaatgcggtc
    80041 agcaccacca gtccggcttc aaccatcaaa ttcgccactt caccgacgcg acggatattt
    80101 tctttacgat cggcatcgct aaaaccgaga tcgctgcata atccgtggcg aacattgtcg
    80161 ccatccagca gatacgtact gacgccgagt ttatgtaacg cctcctccag cgccccggcg
    80221 accgttgatt taccggaccc ggagaggccg gtaaaccaca gcactacacc acgatgaccg
    80281 tggtgtagct cgcgttgttg cacagtgacc ggatggctat gccagacgac gttttcgtca
    80341 tgcagcgcca ttatttatcc cccagcaaat cgcgcgcgcc ccagtgcgga aagtggcgac
    80401 gaaccagtgc attcaattcc agttcgaatg cactgaattc agatggcgca gtagttgcct
    80461 ggctaactgg ctcgtgcacc ataccggcac ctacggtcac attactcagg cgatcgataa
    80521 aaatcagccc accggtaacc gggttttgct gataacgatc taacaccagt ggctcgtcaa
    80581 aggtgagatc caccaagccg atgccgttca gcggcaggtt ttcaacttcg cgttgggtaa
    80641 ggttattaat atcaacctga taacgaatgc catcaacacg agcacgcgtc ttcttaccgg
    80701 caattttgat gtcgtaacgc tggcctgggg aaagcggctg ttccgccatc cataccacat
    80761 ccaccgacgc gctctgcaca gccggtaatg attcgtctgc cgccagcagc agatcgccac
    80821 ggctgatgtc gatctcatcc gtcagcacca gggtgatcgc ttctccggca aaggcttctt
    80881 cgcgatcgcc atcaaaagtc acgatccgcg cgacgtttga ttccacacca gagggcagca
    80941 ctttgacacg ttgcccgact tccacgcgac cggatgccag cgttccggcg tagccacgaa
    81001 aatcgagatt cgggcgatta acgtactgca ccgggaagcg cattggctgg gcatccacca
    81061 ctcgctggat ctccacggtt tccagcactt cgagcagtgt cggaccgctg taccacggca
    81121 tactttcact ttgcgaagcc acgttgtcgc cttccagtgc agagagcggc acaaagcgga
    81181 tatccagatt acccggcagc tgcccggcaa acgtcagata atcttcgcga atacgggtga
    81241 acgtctcttc actgtaaccc accagatcca ttttgttgat cgccacgacc agatgtttga
    81301 tccccaacag tgtggagata aaactgtgac gacgggtttg atcgagcacg cctttacggg
    81361 catcgatcag taagatcgcc agttcacatg tcgatgcgcc agtcgccata ttgcgagtgt
    81421 actgctcgtg ccctggggta tcggcgataa taaatttacg cttctcggta gagaaataac
    81481 ggtaagccac gtcaatggtg atgccctgtt cgcgctcagc ttgcaggccg tccaccagca
    81541 gtgccagatc cagtttttcg ccctgggtgc cgtgacgttt actgtcgtta tgcagcgatg
    81601 agagctgatc ttcgtagatt tggcgggtat cgtgcagcag gcgaccaatc agggtacttt
    81661 tgccgtcatc gacgctacca caggtcagaa aacgcagcag gcttttatgt tgttgcgcaa
    81721 tcatccaggc ttcgacgccg ccttcattgg cgatttgttg tgcaagtgcg gtgttcatct
    81781 taaaaatacc cctgacgttt tttcaactcc atggatcccg cctggtcgcg gtcaatcacg
    81841 cgaccctgac gttcactggt ggtggaaacc agcatctctt cgatgatttc cggcagtgtt
    81901 tgtgcatttg actccaccgc accggtcagc ggccagcagc ccagcgtacg gaaacgcacc
    81961 atccgttttt taatcacttc gcccggttgc aggtcgatac ggttgtcatc aatcatcatc
    82021 aacataccgt cgcgttccag aaccggtcgt tccgcagcga gatacagcgg aacaatgtcg
    82081 atattttcca gccagatgta ttgccagata tcctgctcgg tccagttaga gagcgggaag
    82141 acgcgaatgc tttcgccttt gttaatttgc ccgttgtagt tgtgccacag ctccgggcgc
    82201 tgatttttcg gatcccagcg atggaagcgg tcacggaaag agtagattcg ctctttagcg
    82261 cgggatttct cttcgtcgcg gcgtgcacca ccgaaggcgg catcaaaacc gtatttattc
    82321 agcgcctgtt tcaggccttc agttttcata atatcggtat gtttcgcgct gccatgaacg
    82381 aatggattaa tccccatcgc cacgccttcc gggtttttat gcaccagcag ctcgcagccg
    82441 taggctttag ccgtacgatc gcggaactca tacatctcgc ggaatttcca gccggtatcg
    82501 acatgcagca acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc cagatgcagc
    82561 atgacgctgg aatctttacc gatagagtag agcatcaccg gatttgagaa ttctgccgcc
    82621 acctcgcgaa taatgtggat gctttccgcc tccagttgcc gcaggtgagt aagtcgtatt
    82681 tgatccataa ccgttccttt gcaataccgc tattttcttg ccatcagatg tttcgactat
    82741 agggagcgta agagaacgaa tgaaattacc aattagaatg agtagttcct taacggaata
    82801 acgatttggc aaagctaata tcaaaaagtg cttaaggcac cggatttcgg gcgtttagga
    82861 agatttgaaa ttgttttagc gcagcggcag tttcatacta tggcggtaaa aaaatttgca
    82921 tggtatttaa ggactcacta tgttttccgc attgcgccac cgtaccgctg ccctggcgct
    82981 cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct aaacctggcg attttgctaa
    83041 tactcaggca cgacatattg ctactttctt tccgggacgc atgaccggaa ctcctgcaga
    83101 aatgttatct gccgattata ttcgccaaca gtttcagcaa atgggttatc gcagtgatat
    83161 tcggacattt aatagtcggt atatttatac cgcccgcgat aatcgcaaga gctggcataa
    83221 cgtgacggga agtacggtga ttgccgctca tgaaggcaaa gcgccgcagc agatcatcat
    83281 tatggcgcat ctggatacat acgccccgct gagcgatgct gacgccgatg ccaatctcgg
    83341 cgggctgacg ttacaaggaa tggatgataa cgccgcaggt ttaggtgtca tgctggaact
    83401 ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg cgaccagtgg
    83461 tgaagaggaa gggaaattag gtgctgagaa tttactcaag cggatgagtg acaccgaaaa
    83521 gaaaaatacg ctgctggtga ttaatctcga taacttaatt gttggcgata aattgtattt
    83581 caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca gggcgctggc
    83641 aattgcgcgt agtcatggaa ttgccgcaac gaccaatccg ggtttgaata aaaattatcc
    83701 gaagggtacc gggtgctgta acgacgcgga agtttttgac aaagccggta ttgcggtact
    83761 ttcggtggag gcaactaact ggaatcttgg gaataaggat ggctatcagc aacgtgcaaa
    83821 aaccgccgcc ttccctgcgg gaacaagctg gcacgatgtc aggcttgata atcaacaaca
    83881 tattgataag gcgcttcctg gaagaataga acgtcgctgc cgtgatgtta tacggataat
    83941 gctaccgctg gtgaaagagc tggcgaaggc gtcttgatgg gtttgaaaat gagagctggg
    84001 agttctaccg caggggcggg gaactccaag tgatgtccat catcgcatcc agtgcgtccg
    84061 gtttatcccc gctgacgcgg ggaacacggc tttaaccaca cgagcaagtc cttcgctggc
    84121 ggtttatccc cgctggcgcg gggaacacag ttaacaaccc gcgccgaaca agcccgacaa
    84181 cggtttatcc ccgctggcgc ggggaacact cgcaccgcgt taatccggca gaaaaacaac
    84241 acggtttatc cccgctggcg cggggaacac ccgacaatct ccgcctccag tcggtcgacc
    84301 tgcggtttat ccccgctggc gcggggaaca ctgtgcccga ctgcgtgccg cgtcgagcgc
    84361 ctgcggttta tccccgctgg cgcggggaac acttcgccgc catcatcctg tggtgccagt
    84421 tgaccggttt atccccgctg acgcggggaa cacgcgcctc ggttgtttag ccatgctcag
    84481 ccccacggtt tatccccgct gacgcgggga acacctttcc acccaggcac tgatactcca
    84541 cataaacggt ttatccccgc tgacgcgggg aacacgatcg ccgggcgcgg ttacccgctg
    84601 cgaaaatcgg tttatccccg ctgacgcggg gaacacctca cgcgaggcat cgatcgtgat
    84661 ctgattgtcg gtttatcccc gctgacgcgg ggaacacata ctcaaaaccc aactcacctg
    84721 ttgttttatc ggtttatccc cgctgacgcg gggaacacta atccggcgaa tctgcatttc
    84781 tacgagccga cggtttatcc ccgctgacgc ggggaacaca ctaagcatac ataactgttt
    84841 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    84901 taaagaacag ataacttatt gattatcaac aggaagaaaa gaaaccaaac gcaccccatc
    84961 caaatccacc ggaatacgtc tattttcacc ccaggtctga aattcaaaac ccgactcggt
    85021 attggtcgcc caggccatta ccacatttcc gcaacctgcc agttgagaga tttgctgcca
    85081 gatcatctcc cgaatacgtt tggacgtatc tccgacataa acaccggcac gcacttccag
    85141 gagccagatc gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caacgacgac
    85201 catactcatc cgccgcgtcc ccggtgaccg ctatcgccca gcgtttcagg ttcagggatg
    85261 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc ggcaagcact
    85321 tcctcgatta acggaattaa tttgcccgtt aacttactgc tacggaaaat atcacgacag
    85381 gccaacctga cttctttatc tggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    85441 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    85501 ccactatgga taaatccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagaa
    85561 ataccgtaca gacatgatgt ggcagcactg atgcagcgat tcacaacatc gcctttttcc
    85621 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    85681 gcatacgtct ggcgaacgcg ggatccctca attccacgta gctgctccac tgaacggcga
    85741 gctggcggtg gctcacgaaa acgtaattca tacattttgc gcaccacctt cagacgtaga
    85801 tcttccgtta aagccagctt tgcctggtag agtaacttat cagcacgcgc cccaccgggt
    85861 tgtccggaag agtagacgcg aacacccgct tcaccgaccc agaccagtag cgtccccacc
    85921 gtggcggcca gatgcaccgc cgcgtgggaa actctcgttc ccggttcgag cataatgcag
    85981 gcgaccgatc ccaccggaat gtgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    86041 ccgtccagta cgtcgatttg accgtactgg aggaagatca tagaggtgcg atcttttaac
    86101 gggatcggac tcagtggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    86161 cacagccgaa cgcgcggctt ttgccatacc cctggctcag acgttgcaga aataacccag
    86221 gatcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    86281 agttttcccg ccgcagttgc tgttgtcggt aggcatcgac cgaggtatcc agtagtgtaa
    86341 aaccgcttct ttccccctgc gctgccagcc agtccagcgc cgcctgttgt tgatgcaacc
    86401 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    86461 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    86521 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    86581 ggcgttcctg cgacaggaca aaaaagcgaa acgccccctg gagttcttcc cgacgataaa
    86641 gaaattgcct ttctttgccg ccaggaaaaa gatcccacag ccactgatgc attacatatt
    86701 caccgcgatc caccagatgc agcaactgcg caggcgaaag ctggccggta tgtaaggtta
    86761 ttcttgagag gtacatggtt cctccttgct gagccacggc ccctgattga tggtgcgttc
    86821 cccaaacagc cactgctgac gatttaaagg aacatcccgg cgacgtaata ttttgctcgc
    86881 gaccaggccg tcgtgttccc cttcccacca gcattcatcc tgaagtttcg ggagtgagac
    86941 tttcagttcg cggaaactat cctgatactg ttggtatgcg ttacgtaaga catcagacgc
    87001 gttgccttcg agcagtaacg gcgcaagtgg taacgccagc ggatgacttt ttcgccccag
    87061 ataaagcgga aaaaccggat gacgtaaacc gtcctgcaac tgttcaagcc tgtaaggcgc
    87121 atcgggggtt gttgccaccg ccaccatcca ccaggcatcg gtgtagtagt cgcgccggga
    87181 gataatcgcg ctcagaagat caggggcgct caactcttcg cgacggctga aataacgcgc
    87241 tttacgcacc tcttttggca tctggaccgt gtgataatcc cgtgcccagc gcgggttacg
    87301 gctggcgcaa accaccagtg aatagtggct gttaaacgcg tttaatcgtt cggtatcatc
    87361 acgccgaatc cctaccccgg cagccagcag ccccagcaat gctgagcgcg aaggcagttc
    87421 atgggtatga cgcacttcgc cgggggcatc gacgccccag gatgccattg gcccatgaag
    87481 ctgaaaaatc aaatattggc tcattagccg ccccttacgc gcagataaag tccagcacgt
    87541 ccttcatgct tccctgtttg ttcatcacgt caaagcttgc gcattcggtc ttctgttcat
    87601 agaccgtatt catattttcg cgaagcgttg taatacgctg caccgccaca tctaactgcc
    87661 gggtgccatt aatgggttca tagaaagccg ccgccagaga gcgtggttgt tcagtgcctt
    87721 tttctgccag cgcccaggag gcgtaggcac ggctggcaaa gctgttctgt ttgccggttg
    87781 gggagacttt aagtgcggct tccgtaaagg cgcgcaaggt ctgattagct aacgcttcgt
    87841 caccgccgag gttttcgacc agcagatctt tatcgatgca gatataggtg tagaacagcg
    87901 cagaaccgaa tccggtttca ccaagatgcc cggcaccggc atcttcagaa gcctggcgca
    87961 aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt ttcactgaca ccaaatgcat
    88021 gcgcgacctg gcaggcggct tcaacattaa attcgggttt attcgccagc atacgaccaa
    88081 acatagcgat atctaccgcc atgcgatctt tacgtaacaa agcgagatct tcctcttttg
    88141 gcgcgcgctt ttcttcggcc agttgatggg ccagcgcttt tacggcgtca aattctgccg
    88201 ggctgatatg gactagctgt tcagtttcgg cgttagtgag cggatctttt ggttttttgt
    88261 cgtttttagc tttcccaaga taatccgcaa tttttgccgc ccattcgatg gcttttttct
    88321 cttcgatgcc tttctcaatc aggatagttg ccgcctcacg cgcaatacgc ccactgcgaa
    88381 taccaatatg gcccgccagt gcctgttcaa aaagtgcaga agtgcgccac gcacgtttca
    88441 gactttgtga ggaaacgcgc agacgcgttg ctccacccag taccacagtt tttggcgcac
    88501 cggtgtcatc acggttaagg ttggcagcag ggtaagcggt taacaaatga agctgaataa
    88561 atgtcgtcat gagatagtcc tttattaatt ttgttcgtta tcggcgtcgc cagcctggta
    88621 atattccagc gcccagcgga tacggataaa ttctgttggc cgctgctggc ggcggtgatg
    88681 attgagcagg tcgtcgctct cctggcacca gcggaaaata tcttctgcca aagagataag
    88741 attgacggaa ccatttaata atttgaccgc gcgacgtaac tgacgcagta aatcatcagg
    88801 cgtttttact gccgacaggc gagtaaaacg cccctccgac attaccgccg ccagctgcgc
    88861 ggcaaaaggc tgccgttcat caatagcttt aacattggcg ctaactgcgg cgaccagcgc
    88921 cagtgcggta aaacgccact cttgtgcttc aattttccac aaagtgtggg tctgcatcag
    88981 cagagaacga aacccttctg acagacaaac atcattaacc gttgtgctac gccgcagact
    89041 ggcacgattg ccgcgttttt cctgcaactc ttcgtgccat ttacgcagcg tggctttgtg
    89101 ctcctctttt acaatactca ttcagcagcc tcctgctttt tctccctggc ggctttagca
    89161 ctttgcttct ccgccgatgt tgtaaaatat ttcttgcgcg cggtcatgac gcgtttcaaa
    89221 tcaacgggct cataaggatt agtgaataca cgctcgtcaa aatcctgacg cgcgaataac
    89281 caaatttcct tttgccattt gccgagtaat tcatccgcat cctgaccttc ttcaatttgg
    89341 cgcactaacc tcaggaagcg atgctgagtt ttgttccaga agtcgatatc cacaaaactg
    89401 aaatcacccc ttgcaccttt tggatcggag aaccatgctt ctttcaatgc actccgtaac
    89461 agactcagaa tccgtgaagc cgtttgcgca gccagccgca gcttcggtat ctggccttct
    89521 tttttattga gcagtagcgg gaaatggtgt tcgtaccaac agcgcgcttt catgttgtca
    89581 aaatcataac caaatcccca taggcccact tttgcctgtt tcagactgct ggcattaaag
    89641 agtttcacca ccagcgcggg aagttccgta ttgttttctg atttacccgt ttcgataagg
    89701 cctaaccagt cgcgccagat taaaccgccc ggttgtggtt taacggagta aaactcgccc
    89761 ccctctttaa gtggtacacg gtaaggcgtt aagggatgct gccacatggc ataattggca
    89821 ccataatttt tggtcgtcat caggctcagg agcgcgtcgt tctgctcgtc gcaaatatcg
    89881 cagttgccga ctgtcgtggt attaaaatca atacgtatac gccgcggcat tccccagtac
    89941 gcctggagtt tattgacctg atcatgggtt accaccgcac cggccagttc gctggtacgc
    90001 gtcgggccta gccaggggaa aatcagatcg tcaaattttt tgggtagcgg taagtcggct
    90061 tcatcctgcg gcatcacgtt gagccatagt ttgcgccaca agggagtttg ttgattgccc
    90121 tgatattcct gtaattcaat cagcgtggtc atcggcccac cgccgcgtaa gccggtgcga
    90181 tagcctttgc cacctgacgg cgcatttaac tgtagagaga acaaggctag tgcagaacac
    90241 tgtgggcaca ggtgttccgt tacaccacgc ttgataaagt ggtctttatt aaactttgtt
    90301 gtttgaacgc cgggaatctc aggcagtagc gaagcgaccg gaactttatc tcccgtgagc
    90361 gcctcaaaat cctgcataaa tgaaggtgaa tctggcccga actggaaagc atggtctaat
    90421 gacagcaatg cttcccgtag cttttcagct tccagtccgt cttcccagat atcatcccaa
    90481 cgacgttgat cttttggcgc gaagctggtt tgtaatagtc ccagcaaaaa ctgccatgcc
    90541 gccccctgga gatctgcccg cggcgcagcg atatcgacaa cattttcatc cgccagatcg
    90601 actggcgcca gtttgcctgt cgttccgtct ttataacgaa cgggcaacca cggggttgtc
    90661 agaagtgaaa acgagttcat acgcatcctc tcgtaaaaaa gaccatccct ggtcggttgt
    90721 ccatcattcc atccttttcg gtgtatcaac tattcagaga gccctgtcgc aggcatctca
    90781 accaaccttg ccagtcaatc cttccatagc cgaatacccg caactttcgt catcggtgac
    90841 taaaatcacg tttgccatct ccggctcttg ccgctgttca atgcaccact gcctgaacgc
    90901 atccccttcc agtaaagaaa actcatcccg atgttttttc caccagcttc gacgcactct
    90961 gacaacgctc atttcccatg cgtgagcacc gttggcataa ggcttcacca caccggcaat
    91021 acaggtagac agccacaggg aaacagactc ctcagccaga cgtgtcgaca gtttttccgg
    91081 gaggtaatcg ttgatattgg cggcatagcc gggcctgaag ttcagcacaa actttttagc
    91141 cattgcgcga tcgcagtaat atttgcccac ttgttcctgc tcgctgcggg caaatccttc
    91201 cggcattacc acgtcctcac cgtagactga ttcaataaga aggcgggctg cgtgtggcat
    91261 ttgaatcgcg ccttgctcac gcagtacacg ctgcgtcagc cagattcgtc catgatcggg
    91321 atagacatat gcactgttac gcatggcact gccgaaccat tcgtcacctg gagcgtcgtc
    91381 ccagacgggg gccagaatca gcaattcagg aggggaacgc tcgtcttttc cgtcacgctt
    91441 taactgacca ttaatatcgc ggatatgccg ctgtaatcgc cccgcccgtt ggatcagcaa
    91501 atcaacaggg gccaggtcgg atatcatttc gtccaggtca atatccacac tttgttcaat
    91561 tacttgtgta caaacaatga cttgcgatga tctctgtaaa ctacaacttt taccgaagcg
    91621 tgccagcgtt tccgtttcaa ttcgctggcg atcgctaaaa gcaaagcggc tatgaaacag
    91681 agaaaggctg gaagcaggaa tgacgccgcg ggcaagcaac tgacgataaa ccttaatagc
    91741 gtcatcgaca gaattccgga tccaggcgat gcatttcccc tgacttactg ccgattcgat
    91801 acgcgcaata cattcttgtt cgctatgaag ccaacccacg ctcacgctac gctcaacgtc
    91861 tttgcgcgtt gctacccggt gtgaatgcac atccgatttc gtaacgtgcg tcagccaggg
    91921 gtaatcatcc ttttccagga acggagcttc ttgctggccc tctgcaccac gcgcaaaggc
    91981 agcaacgagt ttgtcgcgct gctgttgaga taacgtggca gaaagcaaaa taacgctgtt
    92041 tcctccacgc gcctgccgct caatcaatcc ttcaagaatg cacgacatgt aagcgtcaca
    92101 ggcatggatc tcatcagcca gcaggatttt gttactcagt cccagaagcc gcagattatt
    92161 atgtttaaac ggcatcaccg ccatcatcgc ctgatccagc gtgccgacgc caatttcagc
    92221 cagcaacgcc ttcttgttac tgtcggcaaa ccaggccgca catccctgac tgaacgcttg
    92281 ttcatccggt tcttctgacc cgactaaatc accggaccag agtgattcat taaagcggtc
    92341 cattaatgtg cgggcactgt gtgccagcac caggctgggg cgggattctg gcgaatagaa
    92401 agcaagccag gtttttacca gccgatcgta catggcattg gccgttgcca ttgttggcag
    92461 gccaaaaaac aaaccctgtg ctttccccgc agccatcaac ctgtgggcca ggataagcgc
    92521 cgcttctgtt ttacctgcgc cagtcacatc ctccagaata aataactgtg gcccaggctg
    92581 gctgatatcc agatccagca ccttttgctg taacggtgtc gggtgctcaa taaaaggaaa
    92641 cagcgtatta attccggtga aaggcgcggt ttgtgctttt ggaggaaaga cggttaaggc
    92701 gttttgagcc tgaactaaag ccttctgcca gtaatcttta atatccattg cctgtgcaac
    92761 gcgtggaaaa aaccgcgttg acgatcccgt ccagtctgcg agtacgacgg ttgcagagat
    92821 ataccaggaa agttgtttta aaagttcaat gccctcgtca tcatcccaga atgcggggat
    92881 ctcgatgagc ggaaacagta ccttgatttc aaggagaaaa tctcgtgcgg cagctttgtc
    92941 ttcaggcaga aaattatcca gttcatcaat acggtcaggg ggccgaccat gatgcccggt
    93001 agtcatgggc atccacatct ccatcacccg tttaagtttg cgcggagaga gtgaagatga
    93061 aggaagcaat tcttcgtatt cacttaaata atgatgccac agccagtagc ccagcgttga
    93121 atgagagatc ttttcgtaat tctttcttgc gccttccgga accttgagtt gtgggtgcag
    93181 gtaaagttgc tgaaaagagc gggcaaattt tccagtatcg tgccagcaca atagccaggc
    93241 gaaaaattgt gctgcctgct ccgtatcagc gaaacctagt tgacgaaaat agtcagtcag
    93301 cccgaagcaa ttgcttttaa ccattaaata gcccatcgcg gctacatcca gcgaatgcca
    93361 gcaaagaagg tgatagtcgt cgcccccgtc tttctcgccg cgtcgggttt ttccccagaa
    93421 atcaaaaaaa gtcacaatat ttttatcctt cagtaaactt a
//
LOCUS       DAOTRK010000003        92133 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.003, whole genome shotgun sequence.
ACCESSION   DAOTRK010000003 DAOTRK010000000
VERSION     DAOTRK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 92133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.003"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(74..646)
                     /gene="ppiA"
                     /locus_tag="QIE41_000197"
                     /gene_synonym="rotA"
     CDS             complement(74..646)
                     /gene="ppiA"
                     /locus_tag="QIE41_000197"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDS3529131.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            917..2098
                     /gene="tsgA"
                     /locus_tag="QIE41_000198"
     CDS             917..2098
                     /gene="tsgA"
                     /locus_tag="QIE41_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDS3529132.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSIAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            2360..4903
                     /gene="nirB"
                     /locus_tag="QIE41_000199"
     CDS             2360..4903
                     /gene="nirB"
                     /locus_tag="QIE41_000199"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDS3529133.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            4900..5226
                     /gene="nirD"
                     /locus_tag="QIE41_000200"
     CDS             4900..5226
                     /gene="nirD"
                     /locus_tag="QIE41_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDS3529134.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            5352..6158
                     /gene="nirC"
                     /locus_tag="QIE41_000201"
     CDS             5352..6158
                     /gene="nirC"
                     /locus_tag="QIE41_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDS3529135.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            6177..7550
                     /gene="cobA"
                     /locus_tag="QIE41_000202"
     CDS             6177..7550
                     /gene="cobA"
                     /locus_tag="QIE41_000202"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDS3529136.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLSQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            7797..7964
                     /locus_tag="QIE41_000203"
     CDS             7797..7964
                     /locus_tag="QIE41_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDS3529137.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(8071..9156)
                     /locus_tag="QIE41_000204"
     CDS             complement(8071..9156)
                     /locus_tag="QIE41_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDS3529138.1"
                     /translation="MKTYPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTRGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIAQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(9168..10472)
                     /locus_tag="QIE41_000205"
     CDS             complement(9168..10472)
                     /locus_tag="QIE41_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDS3529139.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFVPA"
     gene            complement(10484..10837)
                     /locus_tag="QIE41_000206"
     CDS             complement(10484..10837)
                     /locus_tag="QIE41_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDS3529140.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(10848..11726)
                     /locus_tag="QIE41_000207"
     CDS             complement(10848..11726)
                     /locus_tag="QIE41_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDS3529141.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMHATGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMIDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAFVQFDTIGKNSYYP
                     DEKRIAMLHALRDCGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(11723..12949)
                     /locus_tag="QIE41_000208"
     CDS             complement(11723..12949)
                     /locus_tag="QIE41_000208"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDS3529142.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LSALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVARRGEELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLADSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQIVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(12950..14113)
                     /locus_tag="QIE41_000209"
     CDS             complement(12950..14113)
                     /locus_tag="QIE41_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDS3529143.1"
                     /translation="MFVEALKRQNPALISAALSLWQKGKIAPDSWVIDVDQILKNGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLPLGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLSGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNPLEA"
     gene            complement(14197..14559)
                     /locus_tag="QIE41_000210"
     CDS             complement(14197..14559)
                     /locus_tag="QIE41_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDS3529144.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDAELLAELAQSSHWQAVVQLHQVLLKEFTLEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(14576..15481)
                     /locus_tag="QIE41_000211"
     CDS             complement(14576..15481)
                     /locus_tag="QIE41_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529145.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(15642..16646)
                     /gene="trpS"
                     /locus_tag="QIE41_000212"
     CDS             complement(15642..16646)
                     /gene="trpS"
                     /locus_tag="QIE41_000212"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDS3529146.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(16639..17397)
                     /gene="gph"
                     /locus_tag="QIE41_000213"
     CDS             complement(16639..17397)
                     /gene="gph"
                     /locus_tag="QIE41_000213"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDS3529147.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLATAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGITPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(17390..18067)
                     /gene="rpe"
                     /locus_tag="QIE41_000214"
     CDS             complement(17390..18067)
                     /gene="rpe"
                     /locus_tag="QIE41_000214"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDS3529148.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(18085..18921)
                     /gene="dam"
                     /locus_tag="QIE41_000215"
     CDS             complement(18085..18921)
                     /gene="dam"
                     /locus_tag="QIE41_000215"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDS3529149.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(19028..20314)
                     /gene="damX"
                     /locus_tag="QIE41_000216"
     CDS             complement(19028..20314)
                     /gene="damX"
                     /locus_tag="QIE41_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDS3529150.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKVASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(20406..21494)
                     /gene="aroB"
                     /locus_tag="QIE41_000217"
     CDS             complement(20406..21494)
                     /gene="aroB"
                     /locus_tag="QIE41_000217"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDS3529151.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(21551..22072)
                     /gene="aroK"
                     /locus_tag="QIE41_000218"
     CDS             complement(21551..22072)
                     /gene="aroK"
                     /locus_tag="QIE41_000218"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDS3529152.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(22474..23712)
                     /gene="hofQ"
                     /locus_tag="QIE41_000219"
     CDS             complement(22474..23712)
                     /gene="hofQ"
                     /locus_tag="QIE41_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDS3529153.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADVGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(23624..24028)
                     /gene="hofP"
                     /locus_tag="QIE41_000220"
     CDS             complement(23624..24028)
                     /gene="hofP"
                     /locus_tag="QIE41_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDS3529154.1"
                     /translation="MRGKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGANCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(24018..24458)
                     /gene="hofO"
                     /locus_tag="QIE41_000221"
     CDS             complement(24018..24458)
                     /gene="hofO"
                     /locus_tag="QIE41_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDS3529155.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAFWLMMLVTLIFLSSTHQEESDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(24442..24981)
                     /gene="hofN"
                     /locus_tag="QIE41_000222"
     CDS             complement(24442..24981)
                     /gene="hofN"
                     /locus_tag="QIE41_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDS3529156.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNSLETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(24981..25760)
                     /gene="hofM"
                     /locus_tag="QIE41_000223"
     CDS             complement(24981..25760)
                     /gene="hofM"
                     /locus_tag="QIE41_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDS3529157.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGITSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            25880..28432
                     /gene="mrcA"
                     /locus_tag="QIE41_000224"
     CDS             25880..28432
                     /gene="mrcA"
                     /locus_tag="QIE41_000224"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDS3529158.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(28600..29160)
                     /gene="nudE"
                     /locus_tag="QIE41_000225"
     CDS             complement(28600..29160)
                     /gene="nudE"
                     /locus_tag="QIE41_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDS3529159.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            29480..31615
                     /gene="igaA"
                     /locus_tag="QIE41_000226"
     CDS             29480..31615
                     /gene="igaA"
                     /locus_tag="QIE41_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDS3529160.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEEHSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            31680..32348
                     /gene="yrfG"
                     /locus_tag="QIE41_000227"
     CDS             31680..32348
                     /gene="yrfG"
                     /locus_tag="QIE41_000227"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDS3529161.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            32359..32760
                     /gene="hslR"
                     /locus_tag="QIE41_000228"
     CDS             32359..32760
                     /gene="hslR"
                     /locus_tag="QIE41_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDS3529162.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            32785..33663
                     /gene="hslO"
                     /locus_tag="QIE41_000229"
     CDS             32785..33663
                     /gene="hslO"
                     /locus_tag="QIE41_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDS3529163.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(33797..35521)
                     /locus_tag="QIE41_000230"
     CDS             complement(33797..35521)
                     /locus_tag="QIE41_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDS3529164.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYGYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKSSL
                     PWTGPLRCLIKTALLAAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            35899..37521
                     /gene="pckA"
                     /locus_tag="QIE41_000231"
     CDS             35899..37521
                     /gene="pckA"
                     /locus_tag="QIE41_000231"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDS3529165.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKSLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            37638..37955
                     /locus_tag="QIE41_000232"
     CDS             37638..37955
                     /locus_tag="QIE41_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDS3529166.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            38014..38310
                     /locus_tag="QIE41_000233"
     CDS             38014..38310
                     /locus_tag="QIE41_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3529167.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEEAPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(38342..39694)
                     /gene="envZ"
                     /locus_tag="QIE41_000234"
     CDS             complement(38342..39694)
                     /gene="envZ"
                     /locus_tag="QIE41_000234"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDS3529168.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGATKEG
                     "
     gene            complement(39691..40410)
                     /gene="ompR"
                     /locus_tag="QIE41_000235"
     CDS             complement(39691..40410)
                     /gene="ompR"
                     /locus_tag="QIE41_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDS3529169.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            40638..41114
                     /gene="greB"
                     /locus_tag="QIE41_000236"
     CDS             40638..41114
                     /gene="greB"
                     /locus_tag="QIE41_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDS3529170.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            41211..43532
                     /locus_tag="QIE41_000237"
     CDS             41211..43532
                     /locus_tag="QIE41_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDS3529171.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            43972..44199
                     /gene="feoA"
                     /locus_tag="QIE41_000238"
     CDS             43972..44199
                     /gene="feoA"
                     /locus_tag="QIE41_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDS3529172.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            44216..46537
                     /gene="feoB"
                     /locus_tag="QIE41_000239"
     CDS             44216..46537
                     /gene="feoB"
                     /locus_tag="QIE41_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDS3529173.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVNIELLATRKSVSSCCAASTTGDCH"
     gene            46537..46773
                     /gene="feoC"
                     /locus_tag="QIE41_000240"
     CDS             46537..46773
                     /gene="feoC"
                     /locus_tag="QIE41_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDS3529174.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQALNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKVCLREWWALR"
     gene            46976..47869
                     /gene="rpnA"
                     /locus_tag="QIE41_000241"
     CDS             46976..47869
                     /gene="rpnA"
                     /locus_tag="QIE41_000241"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDS3529175.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGFQKGIRTNALNIAKTMLDAGVS
                     LEDVLRFTSLTVEDLAEINHQ"
     gene            complement(48089..48859)
                     /gene="bioH"
                     /locus_tag="QIE41_000242"
     CDS             complement(48089..48859)
                     /gene="bioH"
                     /locus_tag="QIE41_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDS3529176.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQSLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            48897..49580
                     /gene="gntX"
                     /locus_tag="QIE41_000243"
     CDS             48897..49580
                     /gene="gntX"
                     /locus_tag="QIE41_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDS3529177.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARSDKTLCPQCGLPA
                     THAHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRLLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            49639..50214
                     /gene="nfuA"
                     /locus_tag="QIE41_000244"
     CDS             49639..50214
                     /gene="nfuA"
                     /locus_tag="QIE41_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDS3529178.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            50575..51891
                     /gene="gntT"
                     /locus_tag="QIE41_000245"
     CDS             50575..51891
                     /gene="gntT"
                     /locus_tag="QIE41_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDS3529179.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(51939..54020)
                     /gene="malQ"
                     /locus_tag="QIE41_000246"
     CDS             complement(51939..54020)
                     /gene="malQ"
                     /locus_tag="QIE41_000246"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDS3529180.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLEDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFADCWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRSPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKK"
     gene            complement(54030..56423)
                     /gene="malP"
                     /locus_tag="QIE41_000247"
     CDS             complement(54030..56423)
                     /gene="malP"
                     /locus_tag="QIE41_000247"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDS3529181.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVENTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            57046..59751
                     /gene="malT"
                     /locus_tag="QIE41_000248"
     CDS             57046..59751
                     /gene="malT"
                     /locus_tag="QIE41_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDS3529182.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSQDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALINRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(59794..60810)
                     /gene="rtcA"
                     /locus_tag="QIE41_000249"
     CDS             complement(59794..60810)
                     /gene="rtcA"
                     /locus_tag="QIE41_000249"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDS3529183.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVHGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFICRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVAAEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLVASFSL
                     HEQNIHSLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(60814..62040)
                     /gene="rtcB"
                     /locus_tag="QIE41_000250"
     CDS             complement(60814..62040)
                     /gene="rtcB"
                     /locus_tag="QIE41_000250"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDS3529184.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            62227..63825
                     /gene="rtcR"
                     /locus_tag="QIE41_000251"
     CDS             62227..63825
                     /gene="rtcR"
                     /locus_tag="QIE41_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDS3529185.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PENEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLLQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(63807..64565)
                     /locus_tag="QIE41_000252"
     CDS             complement(63807..64565)
                     /locus_tag="QIE41_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDS3529186.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(64582..65412)
                     /gene="glpG"
                     /locus_tag="QIE41_000253"
     CDS             complement(64582..65412)
                     /gene="glpG"
                     /locus_tag="QIE41_000253"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDS3529187.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADSRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYIWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(65457..65783)
                     /gene="glpE"
                     /locus_tag="QIE41_000254"
     CDS             complement(65457..65783)
                     /gene="glpE"
                     /locus_tag="QIE41_000254"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDS3529188.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            65973..67478
                     /gene="glpD"
                     /locus_tag="QIE41_000255"
     CDS             65973..67478
                     /gene="glpD"
                     /locus_tag="QIE41_000255"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDS3529189.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYSARLRRRYPFLTESLARHYARTYGSNSELLLCNAGAIGDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS"
     gene            complement(67693..68293)
                     /locus_tag="QIE41_000256"
                     /pseudo
     CDS             complement(67693..68293)
                     /locus_tag="QIE41_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(68296..69288)
                     /locus_tag="QIE41_000257"
     CDS             complement(68296..69288)
                     /locus_tag="QIE41_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDS3529190.1"
                     /translation="MNKFTCVLLLGTALLAGCVDREGYYNSVREEDSHGLTSLRGQPE
                     LRYNDDWSRWPRVYGATALYPLYASAYYELVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGTATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDIKNPVNSLSEQQ
                     VKDIFSGKVSRWNKVGGGDEGIKIWQRPEDSGSQTVMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIIHAYMVTHENPTLETQKFVDWFLSPQGQQLVEDVGYVPIYDASSESSGQ"
     gene            complement(69314..70822)
                     /locus_tag="QIE41_000258"
     CDS             complement(69314..70822)
                     /locus_tag="QIE41_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDS3529191.1"
                     /translation="MMQNGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEFC
                     NLLFTPTIYLLLNVFMLTLGALLLFFSGRVWAGDSAPENREIAAWRQCLFLVPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFTVSFVGGEYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFILLLLGIVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYVVDAFMVTRDNMTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTMK"
     gene            complement(70948..73395)
                     /gene="glgP"
                     /locus_tag="QIE41_000259"
     CDS             complement(70948..73395)
                     /gene="glgP"
                     /locus_tag="QIE41_000259"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDS3529192.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(73414..74847)
                     /gene="glgA"
                     /locus_tag="QIE41_000260"
     CDS             complement(73414..74847)
                     /gene="glgA"
                     /locus_tag="QIE41_000260"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDS3529193.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(74847..76142)
                     /gene="glgC"
                     /locus_tag="QIE41_000261"
     CDS             complement(74847..76142)
                     /gene="glgC"
                     /locus_tag="QIE41_000261"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDS3529194.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(76160..78133)
                     /gene="glgX"
                     /locus_tag="QIE41_000262"
     CDS             complement(76160..78133)
                     /gene="glgX"
                     /locus_tag="QIE41_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDS3529195.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCIFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(78130..80316)
                     /gene="glgB"
                     /locus_tag="QIE41_000263"
     CDS             complement(78130..80316)
                     /gene="glgB"
                     /locus_tag="QIE41_000263"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDS3529196.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(80589..81692)
                     /gene="asd"
                     /locus_tag="QIE41_000264"
     CDS             complement(80589..81692)
                     /gene="asd"
                     /locus_tag="QIE41_000264"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDS3529197.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYSHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            81884..82477
                     /gene="yhgN"
                     /locus_tag="QIE41_000265"
     CDS             81884..82477
                     /gene="yhgN"
                     /locus_tag="QIE41_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDS3529198.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(82523..83818)
                     /locus_tag="QIE41_000266"
     CDS             complement(82523..83818)
                     /locus_tag="QIE41_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDS3529199.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTADLRYTQQLAAKLAQQGICVISGGARGIDECAIASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVLVKPNQDVQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISMWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(83725..85821)
                     /locus_tag="QIE41_000267"
     CDS             complement(83725..85821)
                     /locus_tag="QIE41_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HDS3529200.1"
                     /translation="MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRLS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(85961..87301)
                     /gene="gntU"
                     /locus_tag="QIE41_000268"
     CDS             complement(85961..87301)
                     /gene="gntU"
                     /locus_tag="QIE41_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDS3529201.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(87305..87832)
                     /gene="gntK"
                     /locus_tag="QIE41_000269"
     CDS             complement(87305..87832)
                     /gene="gntK"
                     /locus_tag="QIE41_000269"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDS3529202.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(87971..88966)
                     /gene="gntR"
                     /locus_tag="QIE41_000270"
     CDS             complement(87971..88966)
                     /gene="gntR"
                     /locus_tag="QIE41_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDS3529203.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(89190..89885)
                     /gene="yhhW"
                     /locus_tag="QIE41_000271"
     CDS             complement(89190..89885)
                     /gene="yhhW"
                     /locus_tag="QIE41_000271"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDS3529204.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVYQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(90008..91045)
                     /locus_tag="QIE41_000272"
     CDS             complement(90008..91045)
                     /locus_tag="QIE41_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDS3529205.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            91380..91868
                     /gene="yhhY"
                     /locus_tag="QIE41_000273"
     CDS             91380..91868
                     /gene="yhhY"
                     /locus_tag="QIE41_000273"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS3529206.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFIDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        22377 a        24923 c        23018 g        21815 t
ORIGIN      
        1 caagtataag cggcaaatct gggttacgcc gggagagcag aagcgcgggc aagattgccc
       61 gcgcgagaaa tcattacggc aggactttag cggaaaggat aactaccggt tttgacggca
      121 cattctggta cggaccaacg tcatgagtcg gcacctggga aatcttatcg gcaacgtcca
      181 tgcctttcac cactttacca aataccgcgt aaccgaaatc acgctgaccg tggtcaagga
      241 aggcgttatc ggcaacgttg ataaagaact ggctggtggc gctgtctttg tcagcggtac
      301 gcgccatcgc gatggtgcca cgcgtgttgc gcaggccgtt atcggcttca tttttgattg
      361 gcgggtttgg ttttttctgc tgcatctgct cggtgaaacc gccgccctga atcataaagc
      421 caggaatgac gcggtgaaag gtagtgttgt tataaaaacc gctgttcaca taatcgacaa
      481 agttttgcac agacactggc gctttttgtt tatccagttc cagttcgatg ttaccagctg
      541 aggttgtcaa caatacgtgc gggtcccctt tcgctgccat tgctgcggga gaaagagcag
      601 aaagagcgaa aacagcagcc atcgccgcca gggtcgattt gaacatgaga tttccttaac
      661 aaacagagca gaaaaaaagc aagtgaagag attctaaata gccttcaaga acaaggccat
      721 ccctttacct aattttacgt atctgaaata tatgtaacct gacgatcact ttttattctg
      781 tgataaaaat cacctcttaa aatgcaattt agcaaccgat tgcaataaaa catttaatca
      841 gatcacaaaa tcacctaaaa tcgcccgctc gcggcatagc ccgctatgcc attttatttt
      901 tgaacgcagg ccaatcatga ctaacagcaa tcgcatcaag ctcacatgga ttagctttct
      961 ctcctacgca ctgaccggtg cgttggttat tgtcaccggg atggtgatgg gaaatatcgc
     1021 cgattatttc aatctgcctg tttccagtat gagtaatacc ttcaccttcc tcaacgccgg
     1081 cattttaatc tctatcttcc tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca
     1141 gttacgtttt ggctttctcc tgatggtgct ggcggttgcc ggtttgatgt tcagccacag
     1201 cctggcgctg ttctcggcgg cgatgttcat tctcggggtg gtcagcggca tcaccatgtc
     1261 gattggtaca ttcctgataa cacaaatgta tgaagggcgt cagcgcggtt cccgcctgtt
     1321 atttaccgac tccttcttca gtatggctgg gatgattttc ccaatgatcg ccgcgtttct
     1381 actggcgcgc agcattgagt ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc
     1441 tatttttatt ctgaccttcg gctgtgagtt cccggcactg ggcaaacatg caccaaaaac
     1501 ggatgctccg gtagagaaag aaaagtgggg gatcggcgtg ctgtttctct ccattgcggc
     1561 actgtgctac atcctcggtc agttaggttt tatctcctgg gtgcctgagt atgccaaagg
     1621 cctgggcatg agcctgaatg atgctggtac gctggtgagt aacttctgga tgtcatacat
     1681 ggtcggcatg tgggcgttca gctttattct tcgcttcttt gatttgcaac gtatcctgac
     1741 cgtactggcg ggtctggccg cgattctgat gtacgtcttt aacaccggaa caccggcaca
     1801 tatggcgtgg tcaattctcg ccctgggctt cttctccagc gcgatctata ccaccatcat
     1861 caccctgggt tcacagcaga ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac
     1921 ctgcgggacc atcggtacta tgttgacctt tgtggttacc ggcccgattg ttgaacatag
     1981 cggtccgcag gcggcactgc ttacagcaaa cggtctgtac gctgtcgtct ttgtgatgtg
     2041 cttcctgtta ggtttcgtca gccgtcaccg tcagcataac accctgacct ctcattaatt
     2101 gctcatgccg ggcggcgcta tcgtcgtccg gccttttcct ctcttaccct gctacgtgca
     2161 tctatttcta taaacccgtt caatttgtct gttttttgca caaacatgaa atgtcagaca
     2221 attccgtgac ttaagaaaat ttatacaaat cagcaatata ccccttaagg agtatataaa
     2281 ggtgaatttg atttacatca ataagcgggg ttgctgaatc gttaaggtag gcggtaatag
     2341 aaaagaaatc gaggcaaaaa tgagcaaagt cagactcgca attatcggta acggtatggt
     2401 cggccatcgc tttatcgaag atcttcttga taaatctgat gcggccaact ttgatattac
     2461 cgttttctgt gaagaaccgc gcatcgctta tgaccgcgta cacctctcgt cttacttctc
     2521 tcaccacacc gccgaagagc tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat
     2581 caaagttctg gtcggcgaac gcgctatcac catcaaccgt caggagaagg tgattcactc
     2641 cagcgccgga cgtaccgttt tttatgacaa gctgatcatg gcgaccggtt cctacccgtg
     2701 gatcccacca atcaaaggtt ctgatactca ggactgcttt gtctatcgca ccattgaaga
     2761 cctcaacgct atagaatcct gcgcccggcg cagcaagcgt ggtgccgttg taggtggcgg
     2821 cctgttaggt ctggaagccg ctggcgcgct gaaaaactta ggcattgaaa cccacgttat
     2881 cgaatttgcc cctatgctga tggcagaaca gcttgatcag atgggcggcg agcagctgcg
     2941 tcgcaaaatc gaaagtatgg gcgtgcgcgt tcacaccagc aaaaacaccc ttgagattgt
     3001 gcaggaaggt gttgaagcgc gtaaaaccat gcgttttgcc gacggcagcg aactggaagt
     3061 cgactttatc gtcttctcta ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg
     3121 tctggacgtt gctccgcgtg ggggtatcgt cataaatgat tcctgccaga cttccgatcc
     3181 ggatatctac gccatcggtg aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc
     3241 accaggctac aaaatggcgc aggtcgccgt tgaccatatt cttggcagcg aaaacgcctt
     3301 tgaaggtgct gaccttagcg ccaagctgaa actgctgggc gtagatgtag gtggtattgg
     3361 tgatgcgcac ggtcgcacgc ctggcgcacg tagctacgtt tacctcgacg aaagcaaaga
     3421 gatctacaaa cgcctgattg tcagcgaaga caacaaaacc ctgctcggtg cggtactggt
     3481 gggcgatacc agcgactacg gcaacctgct gcaactggtg ctgaacgcta tcgagctgcc
     3541 ggaaaacccg gattctctga ttctgccggc gcactctggt agcggtaagc cgtctatcgg
     3601 tgttgataaa ctgccggaca gcgcgcaaat ctgctcctgc ttcgacgtca ccaaaggcga
     3661 cctgattgct gctatcaaca aaggctgcca caccgttgcg gcgctgaaag ctgaaaccaa
     3721 agcgggtact ggctgcggtg gctgtatccc gctggtcact caggtactga acgcggaact
     3781 ggcgaaacag ggcatcgaag ttaacaacaa cctgtgcgaa cactttgctt attcgcgtca
     3841 ggaactgttc catttaatcc gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa
     3901 acacggcaaa ggctacggtt gtgaagtttg taaaccgacc gtcggttcgc tgctggcctc
     3961 ctgctggaac gaatacattc tgaagccgga acatactccg ctgcaggatt caaacgacaa
     4021 cttcctcgct aacatccaga aagacggcac ctactcggtg atcccgcgtt ctccgggcgg
     4081 tgaaatcacc ccggaagggc tgatggcagt aggtcgtatc gcgcgtgaat ttaatctcta
     4141 caccaaaatc accggctccc agcgcctggc gatgtttggc gcacagaaag acgatctgcc
     4201 ggagatctgg cgtcagctga ttgaagccgg cttcgaaacc ggtcatgcct atgcgaaagc
     4261 actgcgtatg gcgaaaacct gcgtgggtag cacctggtgc cgctacggcg ttggcgatag
     4321 cgtcggcctc ggcgtggaac tggaaaaccg ctacaaaggc atccgtacgc cgcacaaaat
     4381 gaagttcggt gtctccggct gtacccgtga atgttcagaa gctcagggta aagacgtggg
     4441 tattatcgcc accgaaaaag gctggaacct gtacgtttgc ggcaacggcg gcatgaaacc
     4501 gcgtcatgcg gatctgctgg cggcggatat cgatcgcgaa acgctgatca aatatctcga
     4561 ccgcttcatg atgttctaca tccgtactgc cgacaaactg acgcgtaccg cgccgtggtt
     4621 agaaaacctt gaaggcggca tcgattacct gaaagcggtg atcattgacg ataaactggg
     4681 gctgaacgca catctggaag aagagatggc gcgcctgcgt gaagcggtag tatgtgagtg
     4741 gactgagacg gtcaatacac cgtccgcgca gactcgcttc aaacacttca tcaacagcga
     4801 caagcgtgac ccgaacgtgc agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc
     4861 gtatgaacgt atcccggtaa ctctggtgga ggacaacgca tgagccagtg gaaagacatc
     4921 tgcaaaatcg atgacatcct gcctgaaacc ggcgtctgcg cgctgttagg tgacgagcag
     4981 gtagcaattt tccgcccgta tcacagcgac caggtgtttg cgatcagcaa catcgacccg
     5041 ttcttcgagt ccagcgtgtt gtcacgcggg ctgattgcgg aacaccaggg cgagctgtgg
     5101 gtcgccagcc cgctgaaaaa acagcgtttt cgcttaagcg acggcttgtg catggaagac
     5161 gaacagtttt ccgtcaagca ttacgaagcg cgagtgaaag acggcgtggt acagctgcgc
     5221 ggttaatgtt ttaacgggag gcgcaatgcc tccccttttt gcattgtcct gtaatgacct
     5281 tcggtatatt gcaggacatt ttttaaactt tttgttttat tttttgtttt tattttttaa
     5341 aggataatca aatgtttaca gacactatta ataagtgtgc ggctaacgct gcgcgcattg
     5401 cacgcctgtc ggcaaataac ccgctcggct tctgggtcag ctccgccatg gcgggcgcgt
     5461 atgtgggtct tgggatcatc ctgattttca cgctcggtaa tttgctcgac ccgtccgtac
     5521 gtcctctggt gatgggcgcg acctttggta tcgccttaac gctggtcatt attgctggtt
     5581 ctgagctgtt caccggacac accatgttcc tcacctttgg agtgaaagcg ggcactatca
     5641 gccacgggca aatgtgggca atcctgccgc aaacctggct gggtaacctg gtcggttccg
     5701 tcttcgttgc catgctctat agctggggtg gcggtagcct gctgccggta gataccagca
     5761 tcgttcactc cgtcgcgctg gctaaaacca ctgcaccggc aatggtactg ttcttcaaag
     5821 gtgcattgtg taactggctg gtttgcctgg ctatctggat ggcgctgcgc actgaagggg
     5881 cggcgaaatt tatcgctatc tggtggtgtc tgctggcatt tatcgcgtcc ggctacgagc
     5941 actccatcgc taacatgacg ctgttcgccc tctcctggtt tggcaaccac agcgaagcct
     6001 acacgctggc gggtattggt cataacctgc tgtgggtgac gctgggtaat actttatcag
     6061 gtgccgtatt catgggattg ggttattggt atgctacgcc gaaagcgaat cgtccggttg
     6121 cggacaaatt taatcaaact gaaacagctg ccggttaatt actaaggggt ttttacgtgg
     6181 atcatttgcc tatattttgc caattacgcg atcgcgactg tctgattgtc ggcggtggtg
     6241 atgtcgcgga acgcaaagca aggttgctgt tagacgcagg cgctcgctta acggtgaatg
     6301 cattagcgtt tattccacag ttcaccgcat gggcagatgc aggcatgtta accctcgtcg
     6361 aagggccatt tgatgaaagc cttctcgaca cctgctggct ggcgattgca gcgacggatg
     6421 atgacgcgct taaccagcgc gtcagtgaag ccgctgaagc tcgtcgcatc ttctgtaacg
     6481 tggtcgatgc gccgaaagcc gccagcttta ttatgccgtc gattattgac cgctcaccgc
     6541 tcatggtcgc ggtctcctct ggcggcacct ctccggttct ggcacgcctg ttgcgtgaaa
     6601 aacttgaatc actgctgccg ttgcatctgg gccaggtagc gaaatacgcc gggcaattac
     6661 gcggtcgagt gaaacaacag ttcgcaacaa tgggtgaacg tcgccgtttc tgggaaaaac
     6721 tgttcgttaa tgaccgtctg gcgcagtcgc tggcaaacaa cgatcagaaa gccattactg
     6781 aaacgaccga acaattaatc aacgaaccgc tcgaccatcg cggtgaagtg gtgctggttg
     6841 gcgcaggtcc gggcgatgcc gggctgctga cactgaaagg actgcaacaa attcagcagg
     6901 cagatgtggt ggtctacgac cgtctggttt ctgacgatat tatgaatctg gtacgccgcg
     6961 atgcggatcg cgttttcgtc ggcaaacgcg cgggatacca ctgcgtaccg caggaagaga
     7021 ttaaccagat cctgctgcgg gaagcgcaaa aaggcaaacg cgtggtgcgg ctgaaaggcg
     7081 gcgatccgtt tatttttggc cgtggtggcg aagagctgga aacactgtgc aacgcgggta
     7141 ttccgttctc ggtggttccg ggtattaccg cagcttctgg ttgctctgcc tattcgggta
     7201 ttccgctcac gcatcgcgat tatgcccaga gcgtacgctt aattaccgga cacttaaaaa
     7261 ccggtggcga actggactgg gaaaacctgg cggcagaaaa acagacgctg gtgttctata
     7321 tggggttgaa tcaggccgcg actattcagc aaaagctgat tgaacacggt atgcctggcg
     7381 aaatgccggt ggcaattgtc gaaaacggaa cggcagtcac gcagcgcgtg attgacggta
     7441 cactctcgca gctgggcgaa ctggcgcagc aaatgaacag tccatcgcta attattattg
     7501 gtcgggttgt tggcctgcgc gataaactga actggttctc caatcattaa tgtaacccgg
     7561 gccagagaat tctggccttc ttaatgactc tttttatata ttcagcaaat aaaacataac
     7621 cccttaataa atattatctg ctaatgggtt atattcaccg ttacagcgac ttacaaaatt
     7681 aagccattca ctcactgacg ctaattaaat attcctgtat cgttcttaat gagcagtctg
     7741 cttatattca tataaacaat gaatattaat caatatataa tacataggga atgtaaatga
     7801 acaaatttat taaagttgca ctggtaggtg cagtactggc tacgttaact gcatgtactg
     7861 gtcatatcga aaaccgtgat aagaactgct cttacgacta cctgctgcac ccggcaattt
     7921 ctatttctaa aatcattggc ggttgcggtc ctactgcaca gtaagtctct gacagaaatc
     7981 ggctaacacc ggtttaattg cctgacgccc tcggggttac ccgagggcgt atttatttct
     8041 gcattctttt cgaaaacatc atgcacaaga ttaaatactg gcaatactct cacgcaaaat
     8101 ccgcagcacc gtctcttcac cgctgcgatt tgggttaatg cgaatcgcgc aatgttcgag
     8161 ttgcggattc gcctggcgaa acgttccgga aagacggtaa aagagcggcg ggatttcata
     8221 tttcgactct gcaccgaccg ggtaaggcaa ggcaccgagc ttttgcgcct cttccagtac
     8281 tctggcggca atcggctgat gaaactccac aatcaacacc ttcgactgcg cattggcaat
     8341 gacggcgctt ttcacttccg gaactgcacc accgttgagc aaggccagca accgttcaga
     8401 tacccccgcc tgcaccgcgt gcatcactgg cgcaaacacc agcccacgca agacttccaa
     8461 cgcctgagcg ccctgaatct ggctgccgcc ggagtagagc gtggcgcgaa tacggttaat
     8521 aacatcagca tcgcctacca ccgcgccaac accctctggc ccaaatagct tgaagcagga
     8581 aaatgtcgag acattcgccc cgcattcaca gccaatgcgc gccaccttca tcaccgcata
     8641 gttgtcatcg gttaatgcag gaacgccagc cgagcgcaac gttgccagca catctgccag
     8701 aatgtacccg tcctgcggct gctggcgcgt atgctgcacc agcgcggcat ctggttgttg
     8761 ctcgtcgaca acctgcttca gtgccaacag gtcattgaaa tcagcagtga taagcgtcag
     8821 ccccatctgc gcaataatga cctgagtcgt cgggtaaaca ggcgcgtcat gcaccagaag
     8881 acgctgcccc ggtttgagca aagccgccaa cgccgcacga atcgctccag tacccgcacc
     8941 ctgtaccagc gccgcagcct gtgcgtgaaa tgcatcagcc agcacctgtt ccactcgctg
     9001 ggtaatgcgc ggttgattca gccccggcgt taagcccaaa tccccgcggg taagaaactc
     9061 gctgccggga aaatggcggc aaatcgtgtc caccagcgca aactgctttt gctgcgcctc
     9121 aataagcgtc aggctttgca aaggatacgt cttcataggt gccttcctta agccggaacg
     9181 aacaggccaa accagtaaag aacattcagc agaataccgg tgatcattac tgccacgacc
     9241 ggtgccgcca ttttctgtac cggacggccc agggattcgt tgaggaagta aatggcaact
     9301 gcgatagaaa atccggtata acccgccatt ttaattgctg cgaaaatcga accgaccagc
     9361 aacgccactt ccatcagcat attcatggcg ttacggatgt tatctgacgc attacgcacc
     9421 gacggatagc gccccagcca tttgccgatc gaacgaagca gcaggacttc cgccgaaata
     9481 accactgcgc ctaataccgc tgcaaccatc ggattcggcg agagatagcc caccgcataa
     9541 acaaaggtaa agcccgcaac tgcatacaca ccagtggcta acgcggtggt ggcaatcaac
     9601 ggtacaaaac ccagcccgcg cataaattcc gccagagctg cctggttaat cagcgtttgt
     9661 gattgttccg gcgttacgcc tgcggaatat gctttctcca gtgtgaagat cgacacttca
     9721 ctgccagcaa aaatcttcat gctggcaacg gcggcaatca gcgccccgac aatagcgata
     9781 tacggtaagt ttttgataat ccgcgacgtg cgttcttcaa acaccgaaag cccgctggca
     9841 tcaatatcat tttcatcacg atgacgcagg tcgtgagtta tcgcgatccc cagcagcatc
     9901 accatgccaa taaagatttc gatagattcc ggattgagat gcgggaaata gcgcactacc
     9961 accacacggg tcatcagtac caccacggcg gcgatcagac tttgcttcca gccaaactgg
    10021 tagaaaatcg ccaccaacgg gaacagtgca aaagctgaaa ccaccggcga gcttaattcc
    10081 cccaggctac ctaatacatc caccggtagc gcggtcagca gctggtttac cggtaacagg
    10141 caagtaagga tcaacacgcc ccagatagcg ccaagaccaa acgccatcag gctgtttatc
    10201 gccagtacgc cgaggatatc ggtaggaaga aagagtaacc atgcgttgag cagtccggtt
    10261 ttcagggtaa aagagatccc caccgaagcc acaaaaccaa tgctcagacc aaaagcgata
    10321 ctccccgcct cgcgacggtt catatagcct tcaatcagtt gcggcaggat cgggcggata
    10381 ccgtcatgaa aaacggccgc cgagcgatgc gccagaagcg atgtcatacc cgtcaggcac
    10441 gccaccacga taatctgaat atacagatcc atagtcgtgc cttttatttc agatggttaa
    10501 tcagcatcgg aatcgcgtgt tcgacgtgct caacggaaag cccaaacgcc actttccctt
    10561 cggcgatcat tttggcgata tgttcgtctt tcgctttaat gcccggcttg gcaatggtgc
    10621 aacttttgtt atagccaatc accgcgatgg caatcgacaa tgccgcgccc gcaccggtat
    10681 tacacgcgcc gatgtagtaa tccagttgcc cggatttcac tttcattgcc gcttccatgt
    10741 cgttgtgaat gaaaacttca aaacagccag gagccgtcgc ttcaatggtt tttttaatct
    10801 gctcacgctg taagcctgca acaccaatct ttttcatgag tctgtcctta ttggaaaaat
    10861 tgagagggag tttcacgtaa catcacatcc acatcggcct gactgaatcc tgactggcgc
    10921 aattgcggaa taaaggtggt caataaatag tcatagccat aaccaccgtt ggcttttaaa
    10981 tgggagcggc gcgtaatatc catcgacagc atgacgcggt tcagcaaacc acagtcacgt
    11041 agtgcgtgga gcatcgcaat gcgcttttcg tccgggtagt aactgttctt gccgatggtg
    11101 tcgaactgca caaacgcgcc gagatcgatc atcttcaaaa tgttgtcgag gttgtctttc
    11161 agatcgcagt gaccaacggt gacgcgcgaa agatcaactc cgtgggcttg cagtagcgcc
    11221 agttgttcca gccccatggt gctaaaagag gtatgcgtgg agatcggacg tccggtctgg
    11281 ttatgtgcca gcgcggcggc aataaacacc ttctcttcca gcggcgtaat cttcccttcg
    11341 ctggagccga tctccgcgat gatcccggct ttcagttccg tgccatcgat accctgttca
    11401 atttcatcga tcatctcctg cgccagttcc tgcacgctgc gggtcgccac atgttccggg
    11461 aaaaacgcgt cctggtaata accggtgcag gccactacgt tgatccccgt cgcgtgcatt
    11521 acatcgagca taaattgcgc attgcgcccc atgtaacggt tggtcatctc aatcacatta
    11581 cgcacgccct gggccatcag gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg
    11641 cggcagtcca cgttgttttt aaagccggag agatcaatat gcagatgctc atgggcgagg
    11701 gtgtaacccg tcggatcaaa actcataggg tgtctcctgc aaaccggagg gaggaaagaa
    11761 aagagcgacc attttgtggc ggtggcgcgc ggaaaaattc acatacggta gcccccacat
    11821 cagaaagcgt ggtgcgcaca ccgagctgcg tagcgaccat cccttgctga taaaccagca
    11881 ctggcaccac ttcgcgggta tggtggctgt gaccaatggt cggatcgtta ccgtgatccg
    11941 ccatcacgac caggcaatca tctggctgca tcgcctcaat aagccgggca aggttacggt
    12001 caacgatctg caaacgttcg gcataacgtg cgacgtcttc cgcgtgacca gcgaggtcgg
    12061 tttcctggat attggtgcaa ataaacgccg tcggatgcgt gttaaattcg tcgagggtga
    12121 tatccataat ccgctggcta tccaccaggt tttgccagct cacgccataa ggattgctga
    12181 cgatatccgc caccttaccc accagcacgg ttggcacgcc cgcttcatac agtttttgcg
    12241 gcacttgtac tttctcatcg acgccgtagc ccatatgcac gacctggaag ccgttgtcat
    12301 aagcgccaga acgcggcgcg ttgataccaa taaatcgccc ttctttactt tctgcggcat
    12361 cgagaatgcg ctggctgtcc gctaacaggc caccaaatgt aatgacccga ccgacctgta
    12421 cctgctcacg cacgatacga ccaattttga ttgcgtcgtc aaaggagatc acagagagat
    12481 tggcggtgat gttatagacc tggcctaaat ccgcctcgag attatcgcca atcgcaaccg
    12541 cctgattgac ccacagaaac tgtaactctt cgccacggcg cgcaacctgc cagccagcgg
    12601 aaactaatgc ctgctcaaca cggtcaatca catcgcgaaa aggcatccgc agcggtggta
    12661 acggacgcgt gcctaaaatt tcctgatgcc ccataaaggt atcgccacct tcatgttgca
    12721 gttctgccac cccccaggtt gcggaatctg acggttgcat atcacctggt gcataaccca
    12781 atgcgttgat tagccccagc ttctccagcg ctgatagctg caaatgcggc aactggctca
    12841 ggatgtgacc acaggtattc gccccggcat cctgcggacg caccaacgtg acatctttca
    12901 ttgcccctac gccaaagcta tcaatcacta acaccacaaa tcgcgccatt tacgcctcca
    12961 gagggttgcc gaggctgtca taacgaccga cgatttccgg ttcgccacga tgaataccag
    13021 acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcgaaaacag agcactactg
    13081 cactgcttac cggaaactcg cctgccagcg gcagggtgta gtcgatactg ctgtcatcca
    13141 cagttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc agcgcatgtt
    13201 gcgcgtgacc acgacggtaa taaccgccgc cgtagcagta gctgtcgcca cgaaaatgat
    13261 gggagatttc acttaaccag agcatcgcga tacgttcagg ctggtcgccc tgctggtttg
    13321 ccggaatcgt ccccgtcagc gcatgaccgg gttcggcatg agtcacaccg tattccgcca
    13381 gcaatggcag cgaagtgcag ctggtcgctg aaggcgcgtt cagttgctca atcgcaatac
    13441 cagatttcgc cagttgatcc cgtgcctgta ccagcgtgtg aagattcggt gtcggcaaaa
    13501 ctttcccggc agcctcatcc caaagcaggc aagggaaatg ggttaatccg gctaaatgca
    13561 gccccgacag tttcttaatt tcagcaacca cctcgtgcag cgaatgctgt acaaaaccgc
    13621 tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc acagactgca
    13681 cacgtccggc cttcaccgct gccgcagaaa cttcccgcgc tttgtcgaga gtaaacacgg
    13741 tgatgacgtc ggtgccctgt gcaacggcgt cagcaacctg atgacaaggg atttgtacca
    13801 gatgcccctg atgcgccacc ggcaaaccgg cgcggcgcat gactcgcgcc tctttgtaat
    13861 ccaccgccac aatgccgctg tagcctaatg gcagcagttt ttccgccagc cacggattac
    13921 gaccaaactg cttagtcatc agatacagtt caatgccgta aagccgcgcc gtctcaatca
    13981 gccgcttacc gtttttcagt atctgatcca cgtcgatcac ccagctgtcc ggggcgatct
    14041 tgcccttttg ccacaagctt agtgcggcgg aaattagcgc cgggttctgg cgtttcaatg
    14101 cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc atttttctga
    14161 atatttagat tatgcgacgt ggatttgtcg ggagcttcaa acctcttcgt tagcagccat
    14221 ccataatcca taaaggttgg ccagcaaata gccttcttca cacgggttaa cttccagtgt
    14281 aaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc tggattgcgc
    14341 cagttctgcc agcaattcgg cgtccagcgg ctctatttct tcaccacggc gactgcgcat
    14401 cagtgcgctc gccatatgtg tcatcgccat cgttccttgc tcactacgca ccggcagatg
    14461 gcactctgtt tccagtacgt tgacgacctg catcatgcct ttgcagatgt ccttatcaat
    14521 aacgcctgca tcgcaaagca ggttgagtct ggtttccata tcagatgcct ttcgtttaat
    14581 aactcggttc ctgttcccca ctcaccactc gttgttgatg ggcgagcagg gcgtgtttat
    14641 caacaactgc acgcagcagt tgttgcatcg ggtaatcatc ggcacgcgtc aggaccacgg
    14701 cttcggtggc ctgtaaaaat cgcggatcgt ctgtgagcgg cgtcgcttct aatcccagca
    14761 tggtcagttc gttttccgcc accacgttcc agataaccgc atcgacatcg cctttaacaa
    14821 tgcgttgtaa actctcgtga taagagagat cgacacgttc cacatcacta tcgccaaaaa
    14881 agacatcggt catgattttc tgatccgccg aacggttatc cagccccacg cgcttcacgt
    14941 ttgcggactc gcctttacgg caaatcaact ggtgctcgcc aacgtaggtg tgcggcccca
    15001 gctccagcgc gaggcataac cctttttgcg tgagataact ttccgccgcc agtcgggaaa
    15061 caaccgccat gtcatacacg ccgttaagta aacactccac gcgaatatcc gcgccgcgca
    15121 tgtgcgcata gtaaaaagga ataccatcaa actgagcttt caatccgctc gccaggcctt
    15181 cgtacaaacg ggtatagggc aagggcatcg cgcacaccac attgttgata tccacatgac
    15241 tcagcagcgc tttgttatcc atttccacca gataactgcc attacgccca cggcgttcta
    15301 tccgtatcgc tccgcttgat tccagcgttt tcaacgcggc ctgcgtcagg ccaacggatg
    15361 aacggcattc agttgccagt tcatctatgg ttttcagtcg attaccgcac ttttcaccca
    15421 acaaataacg ggccagtgtc gtgatgacga cgccttcttt tttgataaac gttcgacgca
    15481 tagtaaattt tcaataaagt gaatatttat atcttcataa aaatgaagat aagtggcaac
    15541 tgcgagatgt ggaaacggcg aggcgcttca cattttttcg gattattgca aaaagaggat
    15601 aaaaaaaccg ggtttccccg gtttttggca ttaatgttaa attacggctt cgccacaaaa
    15661 ccaatcgctt cgtacaccgc ttttagcgta cgggaagcgt gcgcgctggc tttttctgcg
    15721 ccatctttca tcacctgttg caggaaggct tcatcgttgc ggaaacggtg ataacgttcc
    15781 tgcaattcag tcagcatacc ggaaacggca tcagccactt cgcctttcag atgaccatac
    15841 atcttgcctt cgaactgttt ttccagttcc gggatgctct ggcccgttac cgcagaaagg
    15901 atatccagca ggttggaaac gcccgctttg ttctgcacgt cgtaacgaac taccggcggc
    15961 tcgtcggagt cggtgaccgc gcgtttgatt ttcttcacta ccgatttcgg atcttccagc
    16021 aggccgataa cgttattgcg gttatcgtca gacttggaca tctttttggt cggctccagc
    16081 agcgacatta cgcgcgcgcc agatttcgga ataaacggct ccggcacttt aaagatctcg
    16141 ccatacagcg cgttgaaacg ctgggcgata tcgcggctca gttccaggtg ctgtttctgg
    16201 tcttcaccca ccggtaccag attagtttga tacagcagga tgtccgcagc catcagcacc
    16261 ggatagtcaa acagaccagc gttgatgttc tcggcataac gcgcagattt atctttaaac
    16321 tgcgtcatgc ggctcagttc gccgaagtag gtatagcagt tcagtgccca gcctaactgt
    16381 gcatgttccg gcacgtggga ctgaacaaaa atggtgcttt tctcaggatc gataccacaa
    16441 gccagataca aggccagcgt atccagcgtc gctttacgca gcttctgtgc atcctggcgc
    16501 acggtgatcg cgtgttggtc aacgatacag taaatgcaat ggtagtcatc ctgcatgttt
    16561 acccactgac gcagcgcacc catgtagtta ccaatggtca attcacctga gggctgtgcg
    16621 ccactaaaaa cgatgggctt agtcattttt cgattcctga ttttcgctat gcggaagccc
    16681 taatgcgggc agaaggtcat ttatagactg ataaattaca tcaggctggc tgagatcgat
    16741 agcctcgccg tagttatatc cgtaggttaa gccaactgat gggcaacctg ccgcttttgc
    16801 tgcctgaata tcattgcgtg agtcgccaac aaacagcatc tgttgtgggg taattcccat
    16861 ccgctcagcc accagtaaca gcgggtccgg atgcggtttt ttgttttgca catcatcacc
    16921 accaatcacc acgctgaagt atttagcgat atctaaggct tccagcagcg gcgcgacgaa
    16981 cggcgtcggt ttgttggtga ccaggcccaa cggcaggcct ttagcctgca atgcgcccag
    17041 cgtatcggca acgtgcggga acaaaaacgt cccctcttcg gcaacctcgc catagtagcg
    17101 atcgaatagt ttacgcagaa tacgtacttg ttcttctgcc ggaatgtcgt catcaacggg
    17161 cggtttaccc attgttttac gcagagtcgc acgttcctga cgcgcccagg tcaatgcgcg
    17221 ctccatcaga acatctgcgc cgttaccaat ccaggttata acgcgttctt cacctgcgac
    17281 gggcaactcc agcgcataca gcgccatatc tactgcggta gccaagccag gagcactgtc
    17341 caccagcgta ccatcgagat caaaagcgac gccgcgaata tcttcaaact tattcatgac
    17401 ttacctttgc cagttcactg cgcatttcat caatgacttt tttgtagtct ggctggtcga
    17461 agattgccga accggcgacg aacatatccg cgcccgccgc agcgatttcg ccaatgttgt
    17521 tcaccttcac gccaccgtcc acttccagtc gaatgtcaaa gccagactcg tcgatacgac
    17581 gacgtacttc gcgcagttta tccagtgttt gcggaatgaa agactgaccg ccgaaaccag
    17641 ggttgacgga catcagcagg atcacatcca gcttatccat cacgtaatcc agatagctca
    17701 gaggtgtcgc cgggttaaat accagacccg ctttacagcc attttcttta atcagttgca
    17761 gcgtgcggtc aacatgctcg gaggcttctg gatgaaaggt aatgatgctg gcaccagcgg
    17821 cagcgaaatc aggcacaatg cgatcgaccg gtttcaccat caggtgtacg tcgatagggg
    17881 cggtaatgcc gtagttacgc aaggatttca gcaccattgg cccaatcgtc agattgggaa
    17941 catagtggtt atccatgacg tcaaaatgca cgacatcagc gccagctgcc agggcttttg
    18001 cggtatcttc acccaggcgg gcaaaatcag ccgacagaat tgagggggca atcaaatact
    18061 gtttcatccg cttctccttg agaattattt tttcgcgggt gaaacgactc ctggtttgta
    18121 caaagccagc agttcgtcca cctttttacg tgtgccgccg ttgctgctta tactgcgtcg
    18181 aactttgacg acatgcaatt ttgcgcgctg ataccactca cgcgttaaca tcgtatcgtg
    18241 attggagatc agcactggaa tatggcgatc aaccagacct tcagcgatct ccgccagatg
    18301 cgcttgttgt tcaagcgtaa aactgtttgt gtgatacgcc gtaaagttgg cggtcgcaga
    18361 cagcggcgca taaggcggat cgcaatagac gacggatgca tcatctgcgc gcgccatgct
    18421 atcggcgtaa gactcacagt agaaaaaggc attctgcgct ttttcagcga agtgatacaa
    18481 ctctgcttcc gggaaatagg gttttttgta gcggccaaac ggcacgttaa actcaccgcg
    18541 cagattgtaa cgacacaggc cgttgtaacc gtagcggttc aaatataaaa acagtaccgc
    18601 ccgacggaac ggatcctggc ttttgttgaa ctcttcgcgg aactgatagt aaacctcggc
    18661 gcaatttgtt tcgggaacaa acagctcgcg tgcggcctgt acgtactcat cagtacgcat
    18721 cttcacaatg ttatagagac tgatcaggtc gctattgata tcggcaagga tataacgaga
    18781 aaagtcggtg ttgagaaaca ccgacccggc acctacaaaa ggctcaacca gacattcgcc
    18841 cttgggcaaa tgccgtttaa tatcatcaag caggggatac ttgccccctg cccacttcaa
    18901 aaaagcgcga tttttcttca tgctgactaa ctaattacac cttctccggc tgtggagaaa
    18961 gctccgacag catccagcgc ttcaggcaac tcccgcaacg ataaaccatt gcgggagata
    19021 accttgatta cttcagatcg gcctgtacct gacgcagcgg tttcgcccac gggtttttgg
    19081 cctggacatc ggctggcaat gtagataccg cttttttcgc ctcttctttc gaggcgtaca
    19141 cgccagaaac caggacatac cacggctgac cattacgcgt cgtttcatag acaacgtagt
    19201 ttttcagatt ctctttcttc gcccaaccgt tcaggttgtc gtagttagag gaactgctca
    19261 gctgcagagt gtaatggctg gacggtgccg atttcaacga accaacatta cctgcggtct
    19321 tccctccagc ggctggtgtt gccgtggttt gcgccgggga tgccgtctgt actggagccg
    19381 tagttgcggt ctcttttggt gctggcgtag aggtcgcagc cggtgccttc gtgctcgcca
    19441 caggagcagc tggttcagta cgcttcggcg tctgtgctac cggcttcggc tccgttttca
    19501 cggttgcttg cggttttttc ggttcaatca ccgcctgctg acgagctggg cgcgtagtgg
    19561 ccggacgttc agcggtttgc gttttcgccg tgtcacgcga tgcattgcca ttgcgaacag
    19621 gcgcgaccgt cgcgggttca gtcggcaacg tggaattgac cgccacatta ttcaactgtt
    19681 gctgattttg tggctgggtc agcgcattgt tcaggtcacc ctgcacttca acacgttgtt
    19741 gaccatccgt tgccaccggg gtttgccctt gagtcggcgt agaagagatc ggcggcagag
    19801 aaacatcctg ctgagtattt tccgcagacg tggttcccgg cgctggctgc acgccattcg
    19861 cctgatcggt cgcattgcca gcaagatcaa tactcttctc gccagacgcg gtttgatcgc
    19921 tggaagaggt cgagggggct tttagcgcag aaccgatacc gatgatcaac agcaggagaa
    19981 ccagaatgcc gacgcccatc atcatatact gacgagaagc gggtttactg gctacttttt
    20041 tgcgcttacg cggacgacgc tctacgcgct cttcatccac ggtttcatct tcggattcgt
    20101 caatttcttc ttcgagttcc ggttcctcat tgcgctcttt ttgcgcacga gtcggacggc
    20161 gatcgtcagt gtcatcaagt tcaatatcat caaaattgat ctgcggttcg ccacgttcag
    20221 tacgctcaga acgttcagaa gattgacgag aacgaccagt acgacgatcg ctgggatcgg
    20281 gtttcagctc gtcttctggt ttgaattcat ccatttaaca ccccactaaa aggttaatgc
    20341 ttaccacgtt gcaattaacc tgaagctaat agaccgcttg ataagcggcc tgacctttct
    20401 tgttgttacg ctgattgaca atcggcaatg gcgttaagaa caagctcgtg cgaaacgccg
    20461 ctgcgaactt cactcttacc aattgccaac ggaagaatta agcgcatctc tcccgcaagg
    20521 actttcttgt cacgcagcat atgcggtaaa tacgcctgcg cggacatttc gcgcggccca
    20581 ttgaccggta acccagcccg cgtgagcagg gttataatac gctgcgtttc ggcagaacta
    20641 aactgcccga gacgttccga cgtccgcgcc gccatcacca tacccgcagc aaccgcttca
    20701 ccgtgtaacc aattgccata ccccatttca gcttcaatgg catgaccaaa ggtatgtccc
    20761 agattcagta aagcacgtaa cccggtttcg cgctcgtcgg cggcgacaac ttctgccttc
    20821 agttcacaac aacggcgaat acagtacgcc attgccggac cgtccagacg caacaacgca
    20881 tccagattct cttccagcca gttgaaaaac gcaccgtcaa gaataatgcc gtatttgatg
    20941 acttctgcca gccccgacgc taactcacgc gggggaagcg ttttcagaca gtcgagatcc
    21001 accaccactg aagcaggctg gtagaacgcg ccaatcatgt ttttaccgag gggatggttg
    21061 accgcagttt tgccgccaac ggaggaatcg acctgcgaca gtaacgtcgt cgggacttga
    21121 atgaaacgta caccgcgctg ataactcgcc gccgcaaagc cggtcagatc gcccactacg
    21181 ccgccgccaa gcgccaccag cgtagtatcg cgaccatgcg gtttttgtaa caacgccgta
    21241 aagacggtat cgagtacagc caggctttta tactgctcgc cgtcagggag gataacgcta
    21301 tcgacgttaa cacccgcctg ttcaagtacg ccgcggacct tatcgagata cagaggagcc
    21361 agggtttcgt tggtaaccaa catgacctgc tcgcccgatt tcagcggtaa gaatgaagct
    21421 ggttcattaa acaaaccaga tgcgatggta attgggtaac tacgttcccc gagagtaacg
    21481 acaatcctct ccataacgcg acatccacct taattactgt acccgcagac gagtgtatat
    21541 aaagccagaa ttagttgctt tccagcatgt gaataatctg gtttgcgacc actttagcgc
    21601 tttgatcatc agtacgaatg gtcacgtcgg caatctcttc atacagcgga ttgcgttcat
    21661 tggccaacgc ttccagaact tcacgcggcg gtgtttcaac gtgcagcaac gggcgttttt
    21721 tatcacgctg cgtgcgtgca agttgctttt cgatggtcgt ttcaagataa acgacaacgc
    21781 cacgtgcgga aagacggtta cgcgtttcgc gggatttcac agagccgccg ccagtagcca
    21841 gcacaatacc ctgtttctcg gtcaactcat tgatgacctt ttcttcgcga tcgcggaagc
    21901 cttcttcgcc ttctaaatcg aaaacccagc ccacatcagc tccggttcgt ttctcaatct
    21961 cttgatcgga atcgtaaaat tccatattga gttgttgagc taactggcgc ccaatagtgc
    22021 tttttccggc acccataggc ccaaccagaa agatattgcg tttctctgcc attttttcgg
    22081 tactactaag actattcgtt aatgataaac ccgcttcgct cagagagcgc cgcaggacat
    22141 gaactgaaac ctcataagat attgcgagag tcagactgaa aattatctca atactccagc
    22201 gggtttggca actgaataaa tcacccatcc ctctgcatat ctggtcgctg caagcgcgct
    22261 gccttgctac caccgctctg gcgataaatc accgggtaag attagcgtaa aaaaagacag
    22321 caaaatgccg cctgaatgat aaatcatcat catggaacgc aggcagcgaa gagcaccaac
    22381 tctcaaatcg gtactccttg tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt
    22441 tcagcgtaaa aacattaccg cttttacggc tgtttactca ctggaaacca gtcgtggcgt
    22501 gataaacacc actaactcgc gtcgttcatc ttcttttccg tcatgacgaa ataattgccc
    22561 gaaccaggga atgtcgccaa gcaacggtac gctatcctga cccgatttat ttttacgggt
    22621 aaaaatgccg cccagcgcca acgtttctcc gcttttgacc tcgacctgcg tttcgatctc
    22681 ctgcttatca atcgccaaca cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt
    22741 ctggctgatg tgtaatttca gccggatgcg acctttttgt aacaccgtgg gcgtaacttc
    22801 catccccagg acggcctctt taaattccac cgacgtcgcg ccactttccc cgctggaaac
    22861 ctgatatgga atttcgctcc cctgtttaat gctggcaggc tgaagatgag aggccagcag
    22921 acgcggactg gcgataatat ccagctgctg tttttgttcg agcgcggaaa gctcaagatc
    22981 cagtaaacgt ccgttgatgc gtccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac
    23041 ggagaggtcg ctgccaagcg tggtgacttg cccaacgcca ccagcttgtt gcgcatcagc
    23101 caacgtccat ttcacgccta actcacgcaa acttttttca ttaatggtga caatatgcgc
    23161 cgacagctca acctgcccga ccggcagatc catttgcgct acccactgtt caagcgcgct
    23221 taacgccgtt ttgttatctc gcagcaaaag gcgattggtg cgtttatcga cggtcatact
    23281 ccctttggca ctcagtagct tctcccccgc tttcgccagt tctcccacgt cggcgtattg
    23341 cagcgttata ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct gctccgcctc
    23401 ctgacgggcg atattgtcat tctgccaggc aacggaatgc accgagagaa tgttgccctc
    23461 ctgacgcgtt atcagtccgg cgcttttcac tacagtttgt agtgcttgct tccagggaac
    23521 atctgttaga tgtaacgaca ccgtaccgct gacgtctggc gacaccacca ggttcaactt
    23581 ctcctgttca gccagcgcct gcaacacctg agctaccgga acgtcatcca ccatcagcgt
    23641 cactttttgc ggctttgccg cctgtacgcc gggtatcagc atcaacagta gtgcggctat
    23701 ccattgcttc atttgtatct ccttgccgtt gccacaacca gtgtggcggt tcgcagtttg
    23761 ccccggttcc cagcgttagt gcgtctggcg tcagctgtaa gatcgtccag ccgttttcca
    23821 gcacatcgtt ttgctgcacc cgtcgccatt ttttttgccc atcttttatt acaccgatga
    23881 tgcgctcgcc tcgccctacc atcccctgat agcgccactg gctaagttcg ctaatccggc
    23941 atagatcttc cggcggttta aaagggtcac gcataccggt taaaaggcac aatgcaatac
    24001 ctgccaacaa ccagcgttta cccctcatgc ggcgtctcca gttgtagcgt gaacaaaaga
    24061 tcatcacctt ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt
    24121 gtaaatgccg acggcactgc ttcccacagc gttttcaacg ccaactcacc tccctgcgcg
    24181 gatggatgcc aggaaaccag ttgcgcgccg gataactgaa aatccagtgg cgaaaagggc
    24241 agcgtttttt cttcgctgaa gggagtggcg tctaccaggc gatacagtgc agcccactgc
    24301 tggtgatgac tcgcccgtag tcgaattaat gcgtcgctct cttcctggtg tgtcgatgac
    24361 agaaaaatga gcgtaactaa catcatcaac cagaatgccc agcaaagctg gcggaggcgg
    24421 ggtgatgtgg cgaaccacca gtcaaagagc atgtccatcg ctaaccttcc ttgttaactg
    24481 atactcaaat tgccagcgtc cctgcgcatc ctgctgcgtg gccccccgct gattgagatg
    24541 aaaagaagca tcctggcgga gtgacgtttc cagcgagttt aacgcggtaa tgcttgttgt
    24601 cagccccttg atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcatgttc
    24661 gggtaaaagc gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg
    24721 gcgctgcctt tgcaaacgct gttcgcgtaa ctgctggcgt tccagcaaac gtggtttcgt
    24781 tatctgtaag ctgcgggcga gttgttgttc cgcctgaagc aaaacggtgt ctacgcgtgc
    24841 ttcggcgttg cttgtcagac gcagtattag cgttatcccg acagccagca gcagaggcgc
    24901 aacgaacatc agcaaccaga aacgaagaaa agcggtccgg cgttgctgtc gccagggcaa
    24961 aaaattaatt ggcgggttca tcagtacacc ttcccaagcg ccagccccag cgcgatggca
    25021 aagtctccac ctggcggcgg tagcggcggc tgacgaacag aaaccgcctc ccaggggtca
    25081 aatccgcctt cgccacatat cgcgacgctt tcgggatcaa cggataacgc tgccgccagc
    25141 tccttcgcgc tagttatccc caccgccagt ttgcgccccc agctatagcg cgtcgcccac
    25201 agccactgtt cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg
    25261 aatcgctgta atgcactggc atccggggtg atcgcgctaa catgaacacg caacctttct
    25321 gccagagtaa gcagcgttgc caactctttg ctttgcgcgg cagtcacgtt ataggcggga
    25381 ctgagtgagt cttcgctgta atcgaagcgc agggagtccg gatccatatc cagctcgcgg
    25441 gccatcgtcc cggacagcca ggccgtttgc tcccgctcac caagggacat cgacgggcgt
    25501 ggaaatgacc gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc
    25561 ggcagttcgc gactccacgg taacaacgtg ttagccagcc gctgcgcatc aacaatccgc
    25621 ccatctttga taatgtcgtg cgccagcggc aaccgccacc agcgttgcaa aaagcattct
    25681 tttgcgcccc gcacgatagc aaccgctacc gcttcttgct gttgtaaatg caaaccaatt
    25741 tgccagatct taaatgccat tgtgatgatc tccttatcac ccgtcactct gacgggtata
    25801 tcaatgcgtc tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa
    25861 actaaatggg aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg
    25921 cattctgctg ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc
    25981 ggatgtggcg acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga
    26041 tggcgagctg atcgctcaat acggtgagaa acgtcgtatt ccggttacgt tggatcaaat
    26101 cccgccggag atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca
    26161 cggcgttgac ccggtgggga tcttccgtgc ggcaagcgtg gcgctgttct ccggccacgc
    26221 gtcacaaggg gcgagtacca ttacccagca gctggcgaga aacttcttcc tcagtccaga
    26281 acgcacgctg atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgct
    26341 gacgaaagac gagatcctcg agctttatct gaacaagatt tacctcggtt accgcgccta
    26401 tggtgtcggt gctgcggcac aagtctattt cggtaaaacg gtcgaccaac tgacgctgaa
    26461 cgaaatggcg gtgatagccg ggctgccgaa agcgccttcc acctttaatc cgctctactc
    26521 aatggatcgt gccgtcgcgc ggcgtaacgt cgtgctatcg cggatgctgg atgaagggta
    26581 tatcacccaa caacagttcg atcagacacg cactgaggcg attaacgcta actatcacgc
    26641 gccggagatt gctttctctg caccgtacct gagcgaaatg gtgcgccagg agatgtataa
    26701 ccgttatggc gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa
    26761 agtacagcag gccgcacagc aggcagtacg taataacgtg ctggactacg acatgcgcca
    26821 cggctatcgc ggcccggcaa atgtgctgtg gaaagtgggc gaatcggcgt gggataacaa
    26881 caagattacc gatacgctga aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt
    26941 caccagcgcc aatcctcagg aagcgacggc gatgctggcg gacgggtcga ccgtcgcatt
    27001 gagtatggaa ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc agggaccgac
    27061 gccgcgtaaa gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg
    27121 cgatgcatgg tggctggcac aagtgccgga agtgaactcg gcactggtgt cgatcaatcc
    27181 gcaaaacggt gccgttatgg cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa
    27241 ccgcgccacc caggcactgc gtcaggtagg ttccaacatc aaaccgttcc tctacaccgc
    27301 ggcgatggat aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttcccgctg
    27361 ggatgcaggt gccggttctg actggcagcc gaagaactca ccaccgcagt atgccggtcc
    27421 aattcgctta cgtcagggac tgggccagtc gaaaaacgtg gtgatggtac gcgcaatgcg
    27481 ggcgatgggc gtcgactacg ctgccgaata tctgcaacgc ttcggcttcc cggcgcaaaa
    27541 cattgtccac accgaatcgc tggcgctggg ttcagcgtca ttcaccccaa tgcaggtggc
    27601 gcgcggctac gcggtcatgg cgaacggcgg cttcctggtg gacccgtggt ttatcagcaa
    27661 aattgaaaac gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga
    27721 atgcgatatt ccggtaattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga
    27781 tgttgaagat gtcgctatct cccgcgagca gcagaatgtt tctgtgccaa tgccgcagct
    27841 ggagcaggca aatcaggcgt tagtggcgaa gactggcgcg caggagtacg caccgcacgt
    27901 catcaacact ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga
    27961 gccaggctgg cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg
    28021 cgggaaaacc ggtaccacta acagttcgaa agatgcgtgg ttttcgggtt acggtccggg
    28081 cgttgtgacc tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac
    28141 ggcttccgga gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca
    28201 gcctgcatgg gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac
    28261 gccgccgccg ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg
    28321 tggcaacagc cgcgaagagt atttcatcga aggtacgcag ccgacacaac aggcggtgca
    28381 cgaggtggga acgaccatta tcgataatgg cgaggcacag gaattgttct gattaaaaag
    28441 gcgcttcagc gcctttttaa gtttgctgac aaagtgcact ttgtttatac cggatacggc
    28501 gtgaacactt tacccgtcta gcaacatcta gaaaaatcaa tatattgcag gagccgcaca
    28561 ggcctgataa gcattatgca tcaggcaaac ttcacgcatt tacaaccgcc cctgcccctt
    28621 caaccattcg cgcacgagga acagcgcgct gacattacgc gcttcgttga agtcagggtc
    28681 ttccagcaaa tccatcatat gcgccagcgg ccagcgcacc tgtggtagcg gctctggctc
    28741 atcgccttcc agcgattccg ggtagagatc ttgcgctacc acgatattca ttttgctgga
    28801 aaagtaagac ggtgccatgc tgagcttctt caaaaaagtc agatcgttcg caccaaatcc
    28861 cacctcttct tttagctcgc ggttcgcggc ttcgaagacg ctttcacccg gatcaattaa
    28921 tcctttcgaa aaacctaatt cgtaggattc agttcccact gcgtattcgc ggatcaggat
    28981 caggtgatcg tccacaatcg gcacaatcat cactgcttcc cggttggttg gacgcattcg
    29041 ttcataaaca cgccgcacgc cattgctgaa ctccagatcc acgctttcga cggtaaacag
    29101 tcgggaacgg gctacagttt caacattcag aatggtgggt ttttgtaatg atttgctcat
    29161 cgtgggatct atgctgtgaa atcagcggtt attgtgcgat atcggacacg ctttcggcaa
    29221 tgtgaattgc atgttattta catttatgta acttaataaa taatcgtcct caaatcaaat
    29281 taaaagtcaa taggttgaaa taactccagg aatttgctga tattccgcct tcagagggtt
    29341 tgctatgatc agcggttact gtgatgtgct taatgatgct taagttaaac tccacgcttg
    29401 ccgatagcca accgcagaat catgtattgt gtccggtgcg actgaccacg cctgacagac
    29461 taagtaagat ggggaaagca tgagcaccat tgtgattttt ttagctgctt tgctggcctg
    29521 ctcactactt gcgggatggc tgataaaagt gcgatccaga cggcgtcagc tgccctggac
    29581 caacgccttc gcggatgcgc aaacgcgtaa actcacacct gaagaacata gcgccgttga
    29641 aaattatctt gagagcctga cgcaggtatt acaggtgcct ggcccaacgg gagccagcgc
    29701 ggcaccgatc tctctggcgc tgaatgccga aagcaacaac gtcatgatgc tgacacacgc
    29761 tatcacgcgt tacggcatct ctaccgacga tccgaataaa tggcgttact acctcgattc
    29821 ggtagaagtc cacctgcccc ccttctggga acagtacatc aacgatgaga ataccgttga
    29881 actgattcat accgattcgc tgccgctggt tatttcactc aacggtcata cgctgcagga
    29941 gtacatgcag gaaactcgcg gctatgcctt gcaacctgtt ccgtcaacac aggcgtcgat
    30001 tcgcggggaa gagagtgagc aaatcgagct actcaatatt cgcaaagaaa cgcacgaaga
    30061 gtatgcgcta agtcgtccgc gcgggctgcg tgaagcgttg ctgattgtcg cctcattcct
    30121 gatgttcttt ttctgcctga ttaccccgga tgtatttgtt ccgtggctgg caggcggcgc
    30181 gttactgctg ctgggcgcag gtctgtgggg gctattcgcg cccccggcaa aatcctccct
    30241 gcgggaaatt cattgtctgc gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga
    30301 tcaggaacag atcaacaata tttcactcgg tattatcgac ctggtctacc ccgcacactg
    30361 gcagccatac attgctcagg atctcggtca acaaaccgat atcgatatct atctcgaccg
    30421 ccacgtagtg cgtcaggggc gatatctttc tctccatgat gaagtaaaaa actttccgtt
    30481 acagcactgg ctgcgcagta cgattatcgc tgcgggctcg ctactggtgc tgtttatgct
    30541 gttattctgg atcccgctgg atatgccgct gaaattcacc ctctcgtgga tgaaaggcgc
    30601 gcagaccatt gaagccacca gcgtgaaaca actggctgac gctggcgtgc gggttggcga
    30661 tacattgcgt attagcggta cgggaatgtg taatattcgc acttccggta cctggagcgc
    30721 gaaaaccaac tcaccttttt taccgtttga ctgctcgcag atcatctgga atgacgcccg
    30781 ctcattgcca ttaccagaat ctgaattggt caacaaagcg acggcattga ccgaagcagt
    30841 taatcgccag ctgcacccta aaccggaaga tgaatctcgc gtcagcgcct cattacgttc
    30901 agcaattcaa aaatccggca tggtattgct tgatgatttt ggcgacatag tactgaagac
    30961 agcggatctg tgttctgcca aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct
    31021 cggcaacagt aaagactggg acgcgctggt aaaacgcgcc aacgccggga agctcgatgg
    31081 cgtgaatgtg ttattacgcc cggtgagtgc ggaatcgctg gataacctgg tggcaacctc
    31141 caccgcgccg tttatcacgc atgaaacggc gcgagcggca caatcactaa acagtccggc
    31201 ccccggcgga ttcctgattg tcagcgacga aggcagcgat tttgttgatc agccctggcc
    31261 ttcggcatca ctttacgact acccgccgca agaacagtgg aacgctttcc agaaactggc
    31321 acaaatgctg atgcatacgc cgtttaacgc cgaaggtatc gtcacaaaaa tcttcactga
    31381 cgccaatggt acgcagcata taggccttca tccgatcccg gatcgttccg gcctgtggcg
    31441 ctatctcagc accacgctgc tgctactgac gatgctgggt agcgccattt ataatggcgt
    31501 acaggcctgg cgtcgttacc agcgtcatcg tactcgcatg atgaagattc aggcctatta
    31561 tgaaagctgc ctgaacccgc aactgatcac cccttcagaa agccttatcg aataacacgt
    31621 ttgcgcggca ggttatgcta ccctgtcgcg caaattgctt cactctggag atttccctca
    31681 tgcatatcaa cattgcctgg caggacgtag ataccgttct gctggatatg gacggcacgt
    31741 tgctcgacct cgccttcgat aattatttct ggcaaaagct ggtgcctgaa acatggggcg
    31801 cgaaaaacgg ggttacgcca caggaagcga tggaatatat gcgccagcaa tatcacgacg
    31861 tacagcatac gctaaactgg tactgtcttg attactggag tgagcaactg ggtctggata
    31921 tctgtgcgat gaccaccgag atgggaccgc gtgccgtact gcgtgaagat accattccgt
    31981 ttcttgaggc actgaaagcc agcggtaagc agcgaatttt gctcaccaat gcgcatccgc
    32041 acaacctggc ggtaaaactt gagcataccg gtctggacgc acaccttgat ttattacttt
    32101 ccacccacac atttggttat ccgaaagagg atcagcggtt atggcatgcg gtggccgaag
    32161 ctacgggtct gaaagctgaa agaacgctgt ttattgatga cagcgaagcg attctcgatg
    32221 ctgccgcgca atttggtatt cgttactgcc tcggcgtgac taatcctgac tccgggattg
    32281 ccgagaaaca gtatcaacgc catccgtcac tgaatgacta ccgccgcctg atcccctcgc
    32341 taatgtgaag gagacgccat gaaagaaaaa cctgctgttg aggttcgact ggataaatgg
    32401 ctatgggctg cccgttttta taaaacccgc gcgctggccc gtgaaatgat tgaaggcggt
    32461 aaggtgcatt acaacgggca gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg
    32521 ctcactctgc gccagggaaa tgacgaacgc acggtgattg taaaggcgat tactgaacag
    32581 cgtcgccccg ccagcgaggc agccttgctg tatgaagaga ctgcggaaag tgtagagaaa
    32641 cgcgaaaaaa tggcgctggc acgtaaactt aatgccttaa ccatgccgca cccggaccga
    32701 cgcccggaca aaaaagagcg ccgcgacctg ttacgattta aacacggcga cagtgaataa
    32761 ctgtcacctg caagagagat gattatgccg caacatgacc aattacatcg ctatctgttt
    32821 gaaaactttg ccgtgcgcgg cgaactggta accgtttcgg aaaccctgca acagatcctt
    32881 gagaatcacg attatccgca gcccgttaaa aacgtgctgg cagaactgct ggttgcgacc
    32941 agcctgttaa ccgctacgct gaagtttgat ggtgatatca ccgtacagct gcaaggcgac
    33001 ggtccgatga atctggcggt tattaacggt aacaacaacc agcagatgcg cggtgtagcg
    33061 cgcgtgcagg gagaaattcc agaaaatgcc gacctgaaaa cgctggtcgg caatggttac
    33121 gtggtgatca ccatcacccc gagcgaaggt gaacgctatc agggcgtggt tggtctggaa
    33181 ggtgataccc tggcggcctg cctggaagat tactttatgc gttctgaaca gctgccgacg
    33241 cgcctgttta ttcgcaccgg cgacgtagac ggcaaaccgg ctgcaggcgg aatgttgttg
    33301 caggtaatgc ctgcgcaaaa tgcccagcag gacgactttg accacctggc gacgctaacc
    33361 gaaaccatca aaaccgaaga attgttgacc ttaccggcaa acgaagtgtt gtggcgtttg
    33421 tatcacgaag aagaggtgac ggtttacgat ccgcaggatg tggagttcaa atgcacctgt
    33481 tcgcgtgaac gttgcgccga cgcgctaaaa acgctgccgg atgaagaagt cgatagtatc
    33541 ctggcggaag atggcgaaat tgacatgcat tgcgattact gcggtaacca ctatctgttc
    33601 aatgcgatgg atattgctga aatccgcaat aacgcctctc cggcagatcc gcaagttcat
    33661 taatgagtat gtccggcagt gagggctctg ccggatttta cgacttaatg gcctgatgcg
    33721 ctgcgcttac caggcctatg aaataaatcg caaattattt tggtcagata cagcgtcgta
    33781 tccgttaaaa gcgttgtcac ttacttaagt ttatttccag cgtttcacca tctaccgtca
    33841 gatcgcgcca cgctttcggc ctcgtcccca gttttccatc cttcgcagct gtgagtaatt
    33901 tttctttcgt tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat tcttttctgt
    33961 ctgagtcaaa gccatagaca atgactctgt catcattaaa agcaaacagg atccgctccg
    34021 cctgaccatc actattcaga tcttgctcga ccaggacgca ggcgtctttt tcaatgcagg
    34081 tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc ggtttaacag
    34141 aaccaggggc aattaacaca ttttcggcta atactttttc cgatatctgt tgctgaagat
    34201 gttgctctcc atctaacgcc atcaacagat cccgcgcgcg tttcgggtct ttcataaacc
    34261 cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctc tgttcgagca
    34321 tatagagact tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc atatgactgt
    34381 tcacgctaat gcgcatactg tccagcaccg gagaattaag aagcaccagt attaccaaca
    34441 ccaataacga aaccgcaagg ttcactttgc cctgaagaac aagggggttt tgccctttac
    34501 gccagacgat actgacaaaa taccccagcg accataccag taacaccagc accgccagcg
    34561 cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat aacgcccagg
    34621 cggcgacgaa cacatacagc ggagcagcca gcagagcggt tttaatcagg caacgcaacg
    34681 gccccgtcca gggaagtgac gatttttgcg gatcgcggac aatagccatt aagatcaatt
    34741 gcaaaaaggc cagcgtcaac agcaacccgg cggcggaaat gtggcgagaa atcgcgctca
    34801 ggcccgtaaa cggcagggtg atgataaaca tcagggttag caatgatact aacggcagca
    34861 accccgtggc gattaacgtg aacaactttt gaatagagtc gattaaacgt gactgtgtac
    34921 gcgcgaggat caccgccagt gcggtaacca gacctaacgt gagataaata aaccagtcgg
    34981 ttgcaaaaaa gagcgttttg aaaaacgtga tgccaacaag tttaaacaac tcaccccaca
    35041 gtaaaagcac cagccacgtc aggccattgg cgagaaaaat caccagtaat ataagtacgt
    35101 tatgccatac tgactggtaa aaatagccat agcggctact gtcattgcgg atgcgaaggc
    35161 tttgttgtat ccacggtagc aacaacattg ccatcagcag cagatagcag ccaaaatccc
    35221 aaagcgcctt ttctgctctc caggggttca tgccatcaat ttgccacttt agccaaccgc
    35281 tcatccctag cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa
    35341 aggagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa
    35401 caatccaact gagatttttt ccggcaagcc aggcgatcag taggtagcaa accagaccct
    35461 gaagtaatcc ggtagcaatc atcccccagc gtgttgcggg tgaaagttca acgttatcca
    35521 cgttagttat ccagaatcaa aaagtgggtt aattatcgca tccgggacgt agtattttgc
    35581 ttttttcaga aaataatcaa aaaagttagc gtggtgaatc gatactttac cgattgaatt
    35641 tgcatcaatt tcattcagga atgcgattcc actcacaata ttcccgccac ataaaccaag
    35701 atttaacctt ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc
    35761 gtttcgtgac aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat
    35821 ctatgagcct tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat
    35881 tggctaagga gcagtgaaat gcgcgttaac aatggtttga ccccgcaaga actcgaggct
    35941 tatggtatca gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag
    36001 gaagagctcg atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc
    36061 gttgccgtcg ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt
    36121 gacgatacca ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac
    36181 aaacctctct ctccggaaac ctggcagcat cttaaaagcc tggtgaccaa acagctttcc
    36241 ggcaaacgtc tgttcgttgt cgatgctttc tgtggtgcga acccggatac tcgtctttcc
    36301 gtccgtttca tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc
    36361 ccgagcgatg aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag
    36421 tgcactaacc cgcagtggaa agaacagggt ctgaattccg aaaacttcgt ggcgtttaac
    36481 ctgaccgagc gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg
    36541 atgttctcga tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc
    36601 gccaacgttg gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa
    36661 accacccttt ccaccgaccc aaaacgccgc ctgattggcg atgacgagca cggctgggac
    36721 gatgacggcg tatttaactt cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa
    36781 gaagcggaac ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc
    36841 gtgcgtgaag atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt
    36901 tcttatccga tctatcacat cgaaaacatc gttaagccgg tttccaaagc gggccacgca
    36961 actaaagtta tcttcctgac cgcagacgcc tttggtgtgc tgccgccggt ttctcgtctg
    37021 actgccgatc aaacccagta ccacttcctc tctggcttca ccgccaaact ggctggtaca
    37081 gagcgcggca tcaccgaacc gacgccaact ttctccgcct gcttcggcgc ggcattcctg
    37141 tcgctgcacc cgactcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcaggcgcg
    37201 caggcgtatc tggttaacac cggctggaac ggcactggca aacgtatctc cattaaagat
    37261 acccgcgcca ttatcgacgc catcctcaac ggttcgctgg ataacgcaga aaccttcact
    37321 ctgccgatgt ttaacctggc gatcccaaca gaactgccgg gcgtagacac gaagattctc
    37381 gatccgcgta acacctacgc ctctccggaa cagtggcagg aaaaagctga aacgctggcg
    37441 aaactgttta tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta
    37501 gcggctggtc cgaagctgta atcagctgaa atctcattga ccccttatcc aaacggataa
    37561 ggggtttttt tatatctaat tagctaccaa tatgtacacg gtacacttat aattatcaat
    37621 agaccgcagg atgcattatg ttcacgttta ttgaactaca aggatttagc aaacgccgcc
    37681 agttattgtt acctgatgat gagttccggg cttttcagga actactcatt caagatccca
    37741 ctgccggtga cattatcgca ggcacaggca gttttagaaa aattcgctgg aaccgttctg
    37801 gaatggggaa acggggtggc attcggataa tttattacaa cgtaacacgc aatggccgaa
    37861 tttatctggc gttgatttat cctaaaaatg aacaggatga tttaacagaa gaacaaagaa
    37921 aggcattgaa actattgtcc gagaagctgc tttaatgttg aagctccaca aattccacca
    37981 tggccgaaat gccaacggag gcattgaaaa cagatgaaag acgaattatt tgctgatctt
    38041 cttgccagcg cagaagaaat ggtacgcatc gaaaagggtg aagaagcgcc tgaaccagag
    38101 cacgtccata cttttagcga aatcgatgta aaagcgatcc gtgaagcaac gggtttacgt
    38161 cagcaagatt ttgctgtcgc cgtaggggta agttatgacc tggtcaaaag ctgggaaacc
    38221 aaacgccgtc aacctaccgg agcaccccga aaattgctcc tgcttttgca agcgaatcct
    38281 ttcattatca atcaattgaa aacaatctaa aaacaggagg caccttcgcc tcccgtttta
    38341 tttacccttc ttttgtcgca ccctgcgccc gcgttaccgg cacaggcagc caggcgcgaa
    38401 tggaaagccc gccccgttcg ctggtgccaa gctccagcat cccgttatgg ttatccacga
    38461 tacgctgcac aatcgccagc cctaatcccg tgccgctaat ggtgcgcgca ctgtcgccgc
    38521 gaacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga ccgtcatctt
    38581 ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc cagccattgc
    38641 cgtaacgggc ggcgttgacc accatattcg ccaccgcacg tttgatcgac agcgggtgca
    38701 ttttcacctc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca tagccacttt
    38761 cggcggcaat cacctcaccg agcactgcgt tgagatccgc catttccatc ggcatctcct
    38821 gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct tcgatatctt
    38881 tattgatcga ttctgccagg tagccatcct gttcgctcat catctcggta gccagacgaa
    38941 tgcgcgtcag cggcgtgcgc agatcatgac ttacccccgc catcagcagc gtgcgatcat
    39001 ccgccagttg cttaacacca gccgccatat ggttaaaggc gcgggtaacg gaacgcacct
    39061 ccgaagcgcc atattcacgc agcggtggcg gtataatccc tttaccgacc tgcaaagcag
    39121 catgttcgag atcaaccaac ggtcggttct ggatacgaat aaacaaccac gccccgccta
    39181 tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agaaaaatcg ccctgatgaa
    39241 tttcggtcag cggcacgcgc acccagatat tgggcgacag ccaggttttc agccagacga
    39301 caggcgaact tttgttgacc tcaacgcgta cttccgtcgg gccgcccagt tgctgcgcca
    39361 tctgatggct taagaattca tagtgttgtg cccaacgcag acctgcctct tcagcagctt
    39421 cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa gcgggaggca
    39481 caaccaactg cgtaccgtcc tccagttgca gtttgtcggt catcaacata cgcacttcgt
    39541 acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc agcaccacca
    39601 gataagtcgt caccaggctg acgaacagca aggtgacgat gagcaataac gtacgggcaa
    39661 atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gaaccgtccg gtacaaagac
    39721 gtagcccaga ccccagacgg tctgaatgta acgcggatgc gccggatctt cttccaccat
    39781 gcggcgtaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggagt attcacggcc
    39841 acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc tgaccaatgc
    39901 cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc ggaacatttc
    39961 acgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt cctgtgacgg
    40021 cgcgcccggc agttcgttgg cctgacgacg cagcaccgca cgaatgcggg ccagcaattc
    40081 acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc caacgatacg
    40141 gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc tctggctacg
    40201 aagacgtcgg caaatcgaca agccatcttc accaggtaac atcaaatcca gtaccataag
    40261 atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga cgcttcgaac
    40321 ctggaagcct tgttcggtga gataacgttc cagcagcgcg cgcaggcgca tatcgtcatc
    40381 gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt cgcaacaatt
    40441 tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg gtatgaattt
    40501 cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt tattcggcga
    40561 aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt atcaaacaga
    40621 caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat gaaaaactca
    40681 aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa aaggtgacct
    40741 gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat aaaaagcgcc
    40801 tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg catggaaaat ctcaaaatcg
    40861 tcgattactc cccgcagcag gaaggcaaag tcttttttgg tgcgtgggtg gagattgaaa
    40921 acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt tttggtcgta
    40981 aagattacat ctctatcgat tccccgatgg ctcgcgcatt gttgaaaaaa gaggtgggcg
    41041 atttggcggt ggtgaatacc cctgccgggg aagcgagctg gtatgttaat gctatcgagt
    41101 acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt ttgccagcca
    41161 gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc atgatgaatg
    41221 attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag gttgacgctg
    41281 ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat cgtaaggaaa
    41341 tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg agctatctgc
    41401 gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa ggcaaactca
    41461 ctgacgagct ggcgaatgcc atcaacgcca ccttaagcaa aaccgaactc gaagacctct
    41521 acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa gcagggcttg
    41581 agccgttggc cgatctgctg tggagcgatc cgtcacacac gccagaagtc gccgctgcac
    41641 aatatattga tgccgataaa ggcgtggcag ataccaaagc cgcgctggac ggcgcgcgct
    41701 atatcctgat ggaacggttt gccgaagatg ccgcgctgct ggcaaaagta cgtgattatc
    41761 tgtggaagaa cgcgcattta gtttctacgg tggtgagtgg taaagaagag gaaggggcga
    41821 aattccgcga ctatttcgat catcacgaac cgctgtcaac ggtgccttct caccgcgcgc
    41881 tggcgatgtt ccgtggtcgt aacgaaggcg tacttcagct ttcgctgaat gccgatccgc
    41941 agttcgatga gccgccaaaa gagagctatt gcgagcaaat cattatggat catctcggcc
    42001 tgcgcctgaa caatgccccg gcggatagct ggcgcaaagg cgtggtgagc tggacgtggc
    42061 gcatcaaggt gctgatgcat ctggaaaccg aactgatggg caccgtgcgc gaacgcgcgg
    42121 aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg gcggccccag
    42181 ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta aaagtagcgg
    42241 tggtcgatgc aactggcaaa ctggtggcga ccgataccat ttacccgcac accggacaag
    42301 ccgcaaaagc agcgatgacc gttgctgccc tgtgtgaaaa gcataatgtt gaactggtgg
    42361 cgatcggcaa cggtacagct tcccgcgaaa ctgagcgttt ctatctcgac gtgcagaagc
    42421 agttcccgaa agtgaccgcg cagaaagtga tcgtcagcga agctggcgcg tcggtttact
    42481 cggcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg cgtggcgcgg
    42541 tgtctatcgc ccgtcgtttg caggatccgc tagcggagct ggtgaaaatc gatccgaaat
    42601 ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcagctggcc cgcaaactgg
    42661 atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacact gcttctgttc
    42721 cgctgttaac tcgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc gttgcctggc
    42781 gcgatgagaa cgggcagttc cagaaccgtc agcaactgtt aaaagtgagc cgtctggggc
    42841 cgaaagcctt cgaacagtgt gcgggcttct tgcgcattaa ccatggtgat aacccgctgg
    42901 acgcctctac cgttcacccg gaagcctatc cggtggtgga acgcattctg gcggcgacgc
    42961 agcaggcact gaaagatctg atgggtaaca gcagcgagct gcgtaacctg aaagcgtctg
    43021 actttactga tgagaaattc ggtgtgccga cagtaactga catcatcaaa gagctggaaa
    43081 aaccaggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat ggcgtcgaga
    43141 caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc ggtgaccaac gtcaccaact
    43201 ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc tcttcattgt
    43261 cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt gtgaaggtga
    43321 aagtgctcga agtcgatctt cagcgtaaac gtatcgccct gactatgcgt ctggacgagc
    43381 agcctggcga aaccaacgcc cgtcgcggcg gcggtaatga acgcccgcaa aacaaccgcc
    43441 cggcagccaa accgcgtggg cgtgaagcgc agcctgccgg taacagcgcg atgatggatg
    43501 cgctggcggc ggcaatgggc aaaaaacgtt aaacgccctg atagacaaac gtaaacctgc
    43561 cagataaagc gttcacgccg catctggcaa cgaacgcctt gcctgacata aaagtgccgg
    43621 agaatatctc cggccttttt attccacaac caaactctca atatatcccg ttaatattta
    43681 tcacttcatt aacaactgaa accttaatta aacattaacc agtcctggta attcgctatt
    43741 cgaattatat tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta
    43801 ttcaaaccaa cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat
    43861 tgtgttgttg attatttaat ctctccttcg ttggcaaatc atctggtctc atgtcgctgt
    43921 caaacgcccc atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac
    43981 actccagata ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc
    44041 caaaaactgc tttctctagg catgttacct ggctcctctt ttaatgtggt gcgcgtcgct
    44101 ccactcggcg accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa
    44161 gatctggcct tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa
    44221 aaaattaacc attggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca
    44281 gctcactggc gcacgtcagc gtgtaggtaa ctgggctggt gttaccgtcg aacgtaaaga
    44341 agggcaattc tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc
    44401 tctgaccacc atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actatattct
    44461 gagtggcgac gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct
    44521 gtacctgacg ctgcaactgc tggaactcgg cattccctgc atcgtggcac tgaacatgct
    44581 cgacattgcc gagaagcaaa atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg
    44641 ctgcccggtg atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc
    44701 gattgatcgc tataaagcta acgagaatgt ggaactggtg cattacgcac agccgctact
    44761 caacgaagca gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg
    44821 ctggctgggc ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc
    44881 gtcgcagcat cttgatgccg ccctcgcccg tctgcgtaat gagatggatg atccggcgct
    44941 gcacattgcc gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac
    45001 cctgacggca gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt
    45061 cctcggtctg ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat
    45121 cggcggggcg ttacagccgc tgtttgacgt cggctccgtg gcgctatttg tgcatggtat
    45181 tcaatggatt ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg
    45241 cctgggtggc ggtattaaca ccgtgctgcc actggtgccg cagatcggca tgatgtacct
    45301 gttcctctcc ttccttgagg actccggcta tatggcgcgt gcggcgtttg tgatggaccg
    45361 tctgatgcag gcgctgggct tgccggggaa atcctttgta ccgctgatcg ttggtttcgg
    45421 ttgtaacgta ccgtcggtaa tgggcgcacg tacgcttgat gcaccgcgtg aacgtctgat
    45481 gaccatcatg atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt
    45541 tgcggctgcc ttcttcgggc agaacggtgc gctggcggtc ttctcgctgt atatgctcgg
    45601 tatcgtaatg gcggtgctga cgggcctgat gctcaagtac accatcatgc gcggtgaagc
    45661 gacgccgttt gtcatggagc tgccggtcta ccatgtacca cacgttaaaa gcctgattat
    45721 ccagacctgg cagcgtctga aaggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt
    45781 cagcattttc ctgagtgctt tcaacagctt ctctctgagc gggaaaatcg tcgataacat
    45841 caacgactcg gcgctggctt ccgtcagccg ggtgatcacc ccagtcttca agccgattgg
    45901 cgtgcatgaa gataactggc aggcaacggt tggcctgttt acaggcgcaa tggcgaaaga
    45961 agtggtggtg ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt
    46021 caatccggcg gaatttaacc tcggtgaaga gctgttcagt gcggtagatg aaacctggca
    46081 gagcctgaaa gacaccttca gccttagcgt actgatgaac ccgattgaag cgagcaaagg
    46141 cgacggcgaa atgggtaccg gggcgatggg tgtgatggat cagaaattcg gtagcgcagc
    46201 tgccgcatac agctacctga ttttcgtcct gctgtacgta ccgtgcatct cggtgatggg
    46261 tgctatcgcc cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa
    46321 tatcgcttac tcgctggcaa cattgttcta tcaagtcgcc agctacagcc agcatccaac
    46381 ttacagcctg gtgtgcattc tggcggttat cctgtttaac atcgtggtta tcggtctgct
    46441 gcgccgcgcg cgtagccggg tgaatatcga actgctggca acgcgcaaat cggtaagcag
    46501 ttgctgcgca gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtacgt
    46561 gatttgctgg cgttacgggg ccgtatggaa gcggcccaga taagccaggc attgaatacg
    46621 ccgcagccaa tgattaacgc catgctgcaa cagctggaaa gcatgggcaa agccgtgcgg
    46681 attcaggaag aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggcaaa
    46741 gtctgtctgc gcgagtggtg ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag
    46801 cccggtatgc gtttgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaattct
    46861 tgcagcgcga cgtaagttcc tctgaaagcg tctcgcaggg atgaaaactg gctaatgcac
    46921 aggtgtggag tggcgcgtag agtcgcggca ttcaaataac aggtgaagga acgccatgag
    46981 caaaaagcag agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga
    47041 gacggcacgc gattttcttg cgtttcattt accggcaccc attcacgcgc tctgtgatat
    47101 gaaaaccctc aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc
    47161 cgacgtgctg tggtcggtga aaacggaaca agggccagga tacatctatt gtctgattga
    47221 acatcaaagc acctcaaaca aactgatcgc atttcgcatg atgcgctacg ccattgccgc
    47281 aatgcaaaat cacctggatg ccggatacaa aaccttgccg atggtggtgc cattgttgtt
    47341 ttaccacggt attgaaagcc cctatcccta ttcgctgtgt tggcttgatt gtttcgccga
    47401 tcccaacctg gcaaggcagc tttatgcctc tgcatttccg ctgattgatg tcaccgtcat
    47461 gcctgatgat gaaatcatgc agcaccgacg tatggcactg ctggagttaa ttcaaaaaca
    47521 tattcgtcaa cgcgacctga tggggctggt agagcaaatg gcctgcttat taagtagtgg
    47581 atacgctaat gacagacaaa tcaaagggct gtttaattac atactgcaaa ctggcgatgc
    47641 ggtacgtttt aacgatttta tcgacggtgt ggccgaacgt tcaccgaaac acaaggagag
    47701 tttaatgact attgcggaaa gattgcggca ggaggggttt cagaaaggta tacgtaccaa
    47761 cgccctaaat attgccaaaa ccatgctgga tgccggagtc tctcttgagg acgttctgcg
    47821 atttaccagc ctgacggttg aagaccttgc tgaaataaat catcaataac gtaaaggatg
    47881 gttttaatga cagatgcaga aagagaatat cttattagtt acctgattgg ttatttattg
    47941 gatcgacgcc tggaagccct ggatatcgcc aaaagaatgc tgtaatccgg agtccatctt
    48001 gcagacaccc tgcgttttac cggggtgtca gaagaagagt tggctgcggc gagtcagtaa
    48061 agttctgtct cgccatttca aaagccacct acaccctctg cttcaacgcc accagcatgc
    48121 gacaaaactc ggccggatgc gaaataaacg gcgcatgggc cgctttggcg aagatgtatg
    48181 attcgctgtg aggccaaagt ttatccagca tcggcaccac tttgcgcggc accagaccgt
    48241 cgagatagcc atacaatcgc aaaaacggca tggacacgtt ttgcagcgac tgacggagat
    48301 cgaccgtttt caggatttcc agcccgccat taagcacgtc aacctccggc atcggtaacg
    48361 ccagaacggt tttcttcagc gcccgcgcat cctggcgggc cgtttcagta cccatggttt
    48421 gtaacgccag gaaccgctcc actgtacgct gaaaatcatc actgagttgc tgctgaaatc
    48481 ccgccagcac gtccggtttt atccccggcc actcgtcacg agcactaaaa caaggtgacg
    48541 acgccacggt gaccagcgcc tgaacacgct cgggatgggt taacgcaatc tggcttgcca
    48601 ccagcccgcc cagactccag cctaaccaaa tggctttatc aggtgcctgt cgcagcacgg
    48661 cttcggccat atcagcaagt gacatcgcac caaatccccg gctacgcccg aagccgggca
    48721 ggtcaacaag gtgcagcgta aaatgcgagc taagttcctc gtcaatgcaa cgccacactt
    48781 cggcattcag tccccatccg tgcaacagca caagatgaac attcccctga cctttggtct
    48841 gccaccagat gttattcatc cgttattgtt ctcttttgac ttacaaggat gaacatatgc
    48901 taacagtacc gggattatgc tggctatgcc gaatgccgct ggcgttaggt cattggggga
    48961 tttgttcggt ctgctcacgc gccgcccgta gcgataaaac gttatgccca caatgtggat
    49021 tacccgctac acacgcccat cttccctgcg gccgctgcct gcaaaaaccg ccgccctggc
    49081 aaagactggt cacggttgcc gactatgcgc cgccgttaag tccgcttatc caccagctta
    49141 agttttcccg gcgcagcgaa atcgccagcg ccctggcacg tctgttacta ttggaagtct
    49201 tacacgctcg tcgcaccact ggcttgcaac tgccggatcg catcatcagc gttccgttat
    49261 ggcagcgacg tcactggcgt cggggattta atcagagcga tttgctgtgt cagccgttat
    49321 cacgcttgtt gcactgccga tgggatagcg aagccgtcac acgaacacgg gccactgcga
    49381 cccagcattt tctcagtgcc cggctgcgca agcgcaacct gaaaaatgcc tttcgccttg
    49441 aattgcctgt gcaaggtcgc catatggtga tagtggatga tgtcgtcacc accggaagta
    49501 ccgtcgcaga gattgcgcag ctgcttttac gcaatggtgc ggcgactgtc caggtctggt
    49561 gcctttgtcg aaccttgtag agcctcgatg atgggcgtat tataaccaac taaaatagtc
    49621 aactattagg ccattactat gatccgtatt tccgatgctg cacaagcgca ctttgccaaa
    49681 ctgctggcaa atcaggaaga agggacacaa atccgcgtat ttgtgattaa ccctggcacg
    49741 cctaacgctg aatgtggcgt ttcttattgt ccgccggacg ctgtagaagc caccgacact
    49801 gccctgaaat ttgacctgct gaccgcgtat gttgatgagt taagcgctcc ttacctggaa
    49861 gatgcagaga tcgactttgt tacagaccag ttgggttccc agttaacgct gaaagcgcct
    49921 aacgccaaaa tgcgtaaagt cgcagacgat gcaccgctga tggagcgcgt ggagtatatg
    49981 ctgcagtcgc agatcaaccc acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa
    50041 atcaccgaag acggttacgc cattctgcaa tttggcggcg gctgtaacgg ttgttccatg
    50101 gtagatgtga cgctgaaaga agggatcgag aagcagctgc tgaacgaatt cccggagctg
    50161 aaaggtgtgc gcgatctcac cgaacaccag cgcggcgagc actcctacta ctaagttatc
    50221 ccctcttttg tagattgccc gatgcgacgc taaagcgtca tttcgggcct acgttgaaca
    50281 tgtgccagac gttggcgcag cattgctttg gcaaatccct cccttcgttt cctcgcgata
    50341 atatgaccaa cctctcataa tttaaattta ccccgctctg gtgattctca aacgccggat
    50401 gttacccgta tcattcacgt gggtaccaaa catactcctg acatctgact acaataatta
    50461 gttttggtgg gtatcagtcg tggtgccgca atatctctgt tcccgattgg gataattaga
    50521 gtttgtcgtc agaaaattga cgttacccat aacaaatgaa aggccaggta aatcatgcca
    50581 ttagtcattg ttgctatcgg tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg
    50641 aacggcttca tcgctctcgt cctcgtggcg cttgctgttg gattaatgca aggaatgccg
    50701 ctggataaag ttattggctc catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc
    50761 ctgatcatgg gttttggcgc aatgctgggc aaaatgctgg cagactgtgg tggcgcacaa
    50821 cgtatcgcca ccacgctgat tgctaaattt ggtaaaaaac acatccagtg ggcggtggta
    50881 ctgaccggtt ttaccgttgg ttttgccctg ttctatgaag tgggtttcgt gctgatgctg
    50941 ccgctggtgt ttaccatcgc ggcttccgcg aatattccac tgctgtatgt tggtgtacca
    51001 atggcggctg cactgtctgt gacccacggc ttcctgccac cgcatccggg tccgactgcg
    51061 attgccacca ttttcaatgc cgatatgggt aaaactctgc tgtacggtac tattctggca
    51121 atcccgaccg tgattctcgc cggtccggtt tacgctcgcg tgctgaaagg tatcgataag
    51181 ccgatcccgg aaggtctgta cagcgcgaaa accttcagcg aagaagagat gccgagtttt
    51241 ggcgtaagcg tctggacctc tctggtcccg gttgtgctga tggcgatgcg tgcgattgcc
    51301 gaaatgatcc tgccgaaagg tcacgctttc ctgccggtag cggagttcct cggtgacccg
    51361 gtaatggcta cgctgattgc cgtactgatt gcgatgttca cctttggtct gaaccgtggt
    51421 cgttcaatgg atcagattaa cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg
    51481 ctgttgatta tcggtggtgg cggtgcgttc aagcaggtgc tggtagacag cggcgtggac
    51541 aaatacattg cttccatgat gcacgaaacc aacatttctc cgctgctgat ggcctggtcg
    51601 attgctgccg tactgcgtat cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt
    51661 ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc cggagctgat ggttattgcg
    51721 gttggttccg gtagtgtgat tttctctcac gtgaacgatc cgggcttctg gctgttcaaa
    51781 gagtacttta acctgactat cggcgagacc atcaagtcct ggtcgatgct ggaaaccatt
    51841 atctcggtgt gcggtctggt aggctgtctg ctgctgaata tggtgatttg ataacaccga
    51901 ctgccggatg catcgcatcc ggcatcaacc gcaccctact acttcttcgc tgccgctctg
    51961 cgccgtctgt ccaaatcctt cagcaacttg ttcacgccat catcggcaaa catcgcctca
    52021 agcgttgcag agagcttgcg ccgccagttt ttatactggt aactggtgcc gggaatattt
    52081 accggttccg ccatatccag ccagtcttct ggctgtagcc ctaacagggc actgttactg
    52141 tcagcgatat aacgttgcaa accacgattc agcgtcggcg tcatcgacat caacgatgcc
    52201 ttatgcccgg cacgttttgg caggcaacca tatttatgca gagcatccag cagtccttgc
    52261 ttcgccagtt cgcgatcctg atacagaccg cgcagtacca cttcatccgg atacaacccc
    52321 agcgttttgc ccagcgttag atccccgctc tcccagtagc cgcgtagcgt aggcaggtcg
    52381 tgtgtcgccg caaccgccat cgactgctct gggtacgctt tcggtgagcg gaatgtcttc
    52441 tcgtggtcgt tttcgaaata gagcactttg taggagtaca caccgctgct gcgcagctta
    52501 ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa tcaccatgca gcgatgacgt
    52561 ttactttcga gtgccagaat cgagagcaga tcatccaccg gatagtgtac atacgcgccc
    52621 tgatccgccg tctcgccata cggtatccac cacaaacgca gcatcgacat cacatggtca
    52681 attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca gctcgataaa cggttcatag
    52741 gcacgcgcgg tgatgatatg cgggtccatt ggcggcaatc cccagttctg ccccaacggg
    52801 ccgaggatat ccggcggtgc gcctaccgac gctttcaggc aatacagttc acggtcgcac
    52861 caggtttccg ccccaccttc cgctacgcca accgccagat cacgatacaa gccaatcggc
    52921 atttcatagc cctggcttat ctcccagcag tcggcaaact ggctgtaagc cagccactgc
    52981 aaccagagat aaaaatcgac gtcatcacga tgttcttcgc agaactggcg cacttctggt
    53041 gaatccacgt tctgatacat ctctggccat gcaggccagc cccagcgcat ttcgtcctct
    53101 ttcacttgct gggcatgtag tgcgtcaaac gccgcctgcc agaacaggct gtcgccttgc
    53161 tcggcaacaa actggcgaaa cgcggtcatc tgttcatcat cacgttgcgc gaaacctttc
    53221 cacgccattc gtaatgctgt catttttagg gcggtaaccg tggagtaatc aacccagtcg
    53281 gcatcccgcg cctgttgcag cgtctgttgc gtggtcggca acttccacca ggcctgagcc
    53341 tcttcgctaa gatggaaatc ttcgacggcg ttaacgtcga tataaatcac attcagccaa
    53401 cggcgagaag acgggctata cgggctggca ctttccggat ttgccggata gagcgcatga
    53461 atcgggttca gaccgatgaa cgacccgcca cgttttgcca catcctcaag catcgctttg
    53521 agatcgccaa aatccccaat accccagttt ttttccgatc gcagcgtata aagctgaacg
    53581 caggcacccc acagcttttg tttattcagc aacgcctgcg gttcgtaaca gcgtttcggt
    53641 gccacaatca cccggcaatg cgcgcgctgg tcgtcctggg tgagtgtcag cgtgtgataa
    53701 ccttccggca gcttcgtcgg tagattgaac gctttgcccc ccgttacatg gcctttgtac
    53761 tgcgttcctt cttcggtggt cagcagccag ctatattcgc cgctgccctc caccaccatc
    53821 ggcatttttt tgccgctggt ataaaccatg acattcggga ctggcgttac cgccactttc
    53881 gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac
    53941 tgcggtttac cgtgggcatt gatgtaattg gggctaatcc ccgccgccag cgcggcatta
    54001 tccagacgtt tgctttccat cgagcttcct tagcgttttg cctgccagat acgagcctga
    54061 taatcgcgaa tagagcgatc cgagctaaac ataccgcagc gggcggtatt gaggatcgcc
    54121 gcgcgggtcc aggcctcctg gtcgcggtac agcacatcca cctgcttttg tgcctctaca
    54181 taagcggcga aatctgccat caccagatac ggatcgccgc cctgtttgcc gatactgtgc
    54241 agcatctggt cgaaggcatg cttatcgccg tcgctgtatt taccgctttc cagctctttc
    54301 aatactgcgt ccagcacctt atctttcttc cgccatttca ccgggtcgta gcctttggcc
    54361 agaattgcct tcacttgttc cacggtatga ccaaaaataa agatattttc ttcaccgact
    54421 ttctcagcga tttcaacgtt cgcaccatcc agcgtaccga cagtaagcgc accattgagc
    54481 gccagtttca tattgccggt accggaagct tctttacctg ccgtcgaaat ttgttcggag
    54541 atatccgccg ccgggatcag tttttccgcc gccgaaacac aataatccgg caagaacacc
    54601 accttcaact tgtcgccaac cagcggatcg ttgttgatca cgtcagccac tttgttgatc
    54661 gcaaagataa tgtttttcgc caggtagtag cctggtgctg ctttcgcgcc gaagaggaag
    54721 acgcgcggta cgcgatcagc ctgcgggttt tcacgaattt ctttgtacaa cgccagaata
    54781 tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa
    54841 atcgcctgtg gattgatctc aataccggta cgaactttca caaactctgc cagacggact
    54901 ttattcgcct gcttgatctc gcgatattgc tgacggaatt tcgcatcatc agcgaatttt
    54961 tccagattga tcagctgatc gagatcgtta gcccactctt ttttcagtga tttatccaac
    55021 agagccgcca gtgccgggtt gcactgtttg atccagcgac gtggggtaat accgttggtg
    55081 acgttatgga atttgttcgg ccatagctgg tgatattccg ggaacagatc tttcaccacc
    55141 agatccgagt gcagcgccgc aacaccgttc accgcgaaac cgccaaccac acacaggttc
    55201 gccatatgca cttgtttgtc gtgcaccacc gccagtttgg cccacacttt ttcatcgccc
    55261 ggccaggtat tttccaccag cgttttaaag cgtgtattaa tttcgttaat aatctgcatg
    55321 tggcgcggca gtaagccttt caccagtttc acatcccagc gttccagcgc ttctggcatc
    55381 agggtatggt tggtgtaagc gaatgttttg ctggtaatgg cccaggcgtc atcccagctc
    55441 atctggtgct catcgatcag cacgcgcagc agttctggaa tcgcgatagt tgggtgggta
    55501 tcgttcagct gaataacttc ataatccgcc agttcgtgca gtttacgccc cgccagatga
    55561 tggcgacgca aaatatccgc taccgaacag gcacactgga agtattgctg catcaggcgc
    55621 agctttttac cggcagtatg gttgtcgttt ggatagagaa ctttggtcag tttttccgcg
    55681 ttgatgccct gctgttcggc acgcaggaaa tcaccatcgt taaatttagt cagatcaaac
    55741 ggatgcgcat gcgtcgcctg ccacagacgc agtggctgcg ccacgccatt acgatagccg
    55801 acaacgggga gatcccacgc ttgaccagta atggtaaatt ccggctccca gcgtccgtct
    55861 ttcgtcactt taccgccaat tccaacctgc acatccagtg cttcattgtg gcggaaccac
    55921 ggatagttac cacgatgcca gtcatccggc gcttcaacct gtttgccatc gacaaatgac
    55981 tggcggaaca agccatattg ataattaagg ccgtagccag tagctgactg cccgacagtt
    56041 gccattgagt cgaggaagca cgccgccaga cgtcccagac caccgttacc cagcgccggg
    56101 tcgatctctt cttccagcaa gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt
    56161 acatcctgat accagccgag attcaacagg ttgttgcccg tcagacgacc aatcaaaaac
    56221 tccattgaga tgtagttaac atgtcgctga ttcgccaccg gtttggcaaa tggctgagca
    56281 cgcagcattt cggccagtgc ttcactcaca gccagccacc actggcgagg agtcatttca
    56341 gccgcagaat ttaagccata acgctgccac tgacgtgaaa gcgcttcctg aaattgctta
    56401 tcgttaaaaa taggttgtga cataggagtt ccacttttct tagattttca acacaacgtt
    56461 atcgctagtt tgccaggctc gatgttgacc ttcctcatcc tgcgggggat taggcaggga
    56521 ggagttgcgg ggatgagcaa ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac
    56581 aggattagca gcaaatcaat gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat
    56641 ggccgttgcg tattttcaaa aagcggaagg taactctata aattaagtaa aggagtgaaa
    56701 cagtttcaca agtaaaatat ccagtgtgct ccatctcatt cttaatagat ttattaagat
    56761 catcttttta gatagcactt tcatcaggaa tgaagaagaa acccttgctt aaatgaatct
    56821 gatgaacata agggaaatac cgtacaaccc agcattcacg ctgtatcagc gtcgttttag
    56881 gtgagtcatt aataaagatt tggaattgtg acagagtgca aattcagaca cataaaaaaa
    56941 cgtcatcgct tgcattagaa aggtttctgg ccgaccttat aaccattaat tacgaagcgc
    57001 aaaaaaaata atatttcctc attttccaca gtgaagtgat taactatgct gattccgtca
    57061 aaattaagtc gtccggttcg actcgaccat accgtggttc gtgagcgcct gctggctaaa
    57121 ctttccggcg cgaacaactt ccggctggcg ctgatcacaa gtcctgcggg ctacggaaag
    57181 accacgctca tttcccagtg ggcggcaggc aaaaacgata tcggctggta ctcgcaggat
    57241 gaaggtgata accagcaaga gcgtttcgcc agctatctca ttgccgccgt gcagcaggca
    57301 accaacggtc actgtgcgat atgtgagacg atggcgcaaa aacggcaata tgccagcctg
    57361 acgtcactct tcgcccagct tttcattgag ctggcggaat ggcatagccc actttatctg
    57421 gtcatcgatg actatcatct gatcactaat ccagtgatcc acgagtcaat gcgcttcttt
    57481 attcgccatc aaccagaaaa tctcaccctg gtggtgttgt cacgcaacct tccgcaactg
    57541 ggcattgcca atctgcgtgt tcgtgatcaa ctgctggaaa ttggcagtca gcaactggca
    57601 tttacccatc aggaagcgaa gcagtttttt gattgccgtc tgtcatcgcc gattgaagcc
    57661 gcagaaagca gtcggatttg cgatgacgtt tccggttggg cgacggcact acagctaatc
    57721 gccctctccg cccggcagaa tacccactca gcccataagt cggcacgccg cctggcggga
    57781 atcaatgcca gccatctttc ggattatctg gtcgatgagg ttttggataa cgtcgatctc
    57841 gcaacgcgcc attttctgtt gaaaagcgct attttgcgct caatgaacga tgcactcatc
    57901 aaccgtgtga ccggcgaaga aaacgggcaa atgcgcctcg aagagattga gcgccagggg
    57961 ctgtttttac agcggatgga tgataccggc gagtggttct gctatcaccc gctgtttggt
    58021 aacttcctgc gtcagcgctg ccagtgggaa ctggcggcgg agctgccgga aattcaccgt
    58081 gctgccgcag aaagctggat ggcccaggga tttcccagcg aagcgattca tcatgcgctg
    58141 gcggcaggcg atgcgctgat gctgcgcgat attctgctta atcacgcctg gagtctgttc
    58201 aaccatagcg aactgtcgct gctggaagag tcgcttaagg ccctgccgtg ggacagtttg
    58261 ctggaaaatc cgcagttagt gttattgcag gcgtggctga tgcaaagcca acatcgctac
    58321 ggcgaagtta acaccctgct agcccgtgct gaacatgaaa tcaaggacat cagagaaggc
    58381 accatgcacg cagaatttaa cgctctgcgc gcccaggtag cgattaacga tggtaatccg
    58441 gatgaagcgg aacggctggc aaaactggca ctggaagaac tgcctccggg ctggttctat
    58501 agccgcattg tagcaacctc agtgctgggt gaagtattgc actgcaaagg cgaattgacc
    58561 cgctcactgg cgttaatgca gcaaaccgaa cagatggcgc gccagcacga tgtctggcac
    58621 tacgccttat ggagtttaat ccagcaaagt gaaattctgt ttgcccaagg gttcctgcaa
    58681 accgcgtggg aaacgcagga aaaagcgttc cagctgatca acgagcagca tctggaacag
    58741 ctgccaatgc atgagtttct ggtgcgcatt cgtgcgcagc tgttatgggc ctgggcgcgg
    58801 ctggatgaag ccgaagcgtc ggcgcgtagc gggattgaag tcttgtcgtc ttatcagcca
    58861 cagcaacagc ttcagtgcct ggcaatgttg attcaatgct cgctggcccg tggtgattta
    58921 gataacgccc gtagccagct gaaccgtctg gaaaacctgc tgggaaatgg caaatatcac
    58981 agcgactgga tctctaacgc caacaaagtc cgggtgattt actggcaaat gaccggcgat
    59041 aaagccgccg ctgccaactg gttgcgtcat acggctaaac cagagtttgc gaacaaccac
    59101 ttcctgcaag gtcaatggcg caacattgcc cgtgcacaaa tcttgctggg cgagtttgaa
    59161 ccggcagaaa ttgttctcga agaactcaat gaaaacgccc gcagtctgcg gttgatgagc
    59221 gatctcaacc gtaacctgtt gctgcttaat caactgtact ggcaggccgg acgtaaaagt
    59281 gacgcccagc gtgtgttgct ggacgcatta aaactggcga atcgcaccgg atttatcagt
    59341 cattttgtca tcgaaggcga agcgatggcg caacaactgc gccagctgat tcaactcaat
    59401 acgctgccgg aactggaaca gcatcgtgcg cagcgtattc tgcgagaaat caatcaacat
    59461 catcggcata aattcgccca tttcgatgag aatttcgttg aacgtctgct aaatcatccg
    59521 gaagtgccag aactgatccg caccagcccg ttgacccaac gtgaatggca ggtactgggg
    59581 ctgatttact ccggttacag caacgagcaa attgcaggcg agctggaagt ggcggcaacc
    59641 accatcaaaa cgcatatccg caatctgtac caaaaactcg gtgtcgccca ccgtcaggcc
    59701 gcggtacaac acgcgcagaa actgctgaag atgatgggat atggggtatg agttcagccg
    59761 gatattacgt cggatccggc ttacatcttt atatcattca atgctcaccc gcgttacgcc
    59821 atcagcctcc accagactaa accgcactgg caagaaacgc tccaccaccg cgatattggt
    59881 cagcagatgg catgagggat gggcgaccgt aaattctccc gcgcccgcca gcgccatcgg
    59941 tagcaccagc tggtcggcga gatattcccc cactgccgcc gggcttgcca ggtagcgttt
    60001 cacctctttc actaactgcg cggctaccac ttcggcactg acgcgctttt caccgacgac
    60061 gaaaaagcgt tcagtgatat tttcactttc gacttccagc gagacggtat tacccggccc
    60121 ctggtcgcgc ggcaggctat gaatattctg ctcatgcagg gaaaaacttg ccaccagcgt
    60181 agcgatttca cgctcagcaa catgcctcgg cacgccagct aatagcacct ctccacgcat
    60241 ctgcacaatg ttcccgcgct cgccaagttg caaggtgtta aacaatgcca cgggcgagac
    60301 ttccgctgcc accaccccgc ctccagcagg ataaaaaccg tggcgtaata atgtggtttg
    60361 ctgatgaatg cccattttcg ccagcagcgg ctccagcacc cgacagataa aatccgcagg
    60421 cggtgccgac gggttatcgg tgccaccgct cacttcaaca cgcgaaggtc catcagcaaa
    60481 ccacagcgcg ggcagcaccg tttgcagtac cagcgtacaa cttccggcgc taccgatagc
    60541 aaagcggtaa tcgccgccgt gcacggtacc tggccggaag accagacgct gcgaccctag
    60601 ctccgcgcct tccaccgttg ccctacaaat ttccgcagcc gcttttaccg cggtcagatg
    60661 ctggcgcaac agccccggtt tcgcccgccc ggcacgaatg ccagtgatgg taaatggctg
    60721 gccagttatc atcgacaggc tcagcgccga gcgcaggatc tgcccgccgc cttcgccctg
    60781 tgcgccatcc agcgcaatca tccttttcat catttatcct tttacgcaca ccacctgacg
    60841 cagggtatag ataacttcca ccagatcgct ttgtgccgcc atcaccgcat caatatcttt
    60901 atacgccatc gggatttcgt cgatcacttc ggcatcttta cggcattcca catgcgcggt
    60961 ggcacgaatt tgatcttcca cgctgaacag ttttttcgct ttggtacggc tcattacccg
    61021 cccggcaccg tggctgcacg aacagaacga ttcttcattt cccagcccac ggacgataaa
    61081 gcttttcgct cccatcgacc ctggaataat tccatattga cccgcacgcg cagacaccgc
    61141 gcctttacgc gtcacgtaga tctcttcacc aaagtgctgt tctttttgca catagttgtg
    61201 atgacagttg atctcttcca tcgccagcgt ttgtggctgt cttaccgttt tctgcgtaac
    61261 gctctgcaac gccgttacca cgttttccat catcgcatcg cggttaaggc tggcaaaaag
    61321 ctgcgcccag gccacggctt tcaggtaatc atcaaagtat tccgtacctt ccataaagta
    61381 cgccagatca cgcgacggca acgtctcaag ttggtcctgc atctcttttt gcgccagatc
    61441 gataaagtaa gtcccgatgg cgttaccaat tccgcgtgaa ccggagtgca gcataatcca
    61501 cacctggtcc gactcatcaa ggcagatttc aataaagtgg ttacccgttc ccagcgttcc
    61561 caggtgttta tagttattgg tattcaggaa acggggatat ttttgcgtta accactgata
    61621 accggcttcc agctcagccc atttcgcatc gacgttaaca ggcggatttt cccatgcgcc
    61681 tttatcacgt ttacaacgtc cggtagtacg tccgtgcggc acggctgttt caatcgcctg
    61741 acgcagctcc gccaggtttt caggcaggtc tgccgccgtt aacgcggtac gcagcgcgtt
    61801 cattccacaa ccgatatcca cgcccaccgc cgccggaata atcgcccctt tggttgggat
    61861 cacgctacca atggtggaac ctttacccag gtgtacatca ggcattaccg caatatgttt
    61921 gaaaataaac ggcatcttcg ccgtattaat aagttgctga cgcgcatcgg cctctaccgg
    61981 cacgcctttg gtccacattt ttaccggggc attttcagtg gtcagtaatt cgtaattcat
    62041 tttgtttttc tcttttcgtt ggtatgtcct gataattgca accgtcgtac cagaaaatta
    62101 aataagcagc ttaatttttt aattcattgt ttttaaaaaa attatacctt tacgtccgta
    62161 aacggagatt tcccgcaaag ccaatttacc gataatgaaa tatcgtcttt tataaggata
    62221 tctaagatgc gtaaaacagt ggcttttggt tttgtcggaa cggtgctgga ttatgccggg
    62281 cgcggcagtc agcgctggtc aaaatggcgt ccgacactct gtctatgcca gcaagaatcg
    62341 ttagttatcg atcggctgga attattgcac gacgcccgct cgcgctcgct attcgaaacg
    62401 cttaaacgcg atatcgccag cgtttcgcca gaaacagaag tggtgggcgt tgagatagaa
    62461 ctgcataacc cgtgggattt cgaagaggtt tacgcctgcc tgcatgattt cgcccgtggt
    62521 tacgcgttcc agccagaaaa tgaagattat ttaattcaca tcaccaccgg cacccacgtc
    62581 gcgcagattt gctggtttct actggcagaa gcgcgttacc tgcccgcccg gttgatacaa
    62641 tcttcaccac cgcgcaaaaa agaacaaccg cgcggcgcag gtgaagtgac gattatcgat
    62701 ctcgatttaa gccgttataa cgctatcgcc agccgctttg ccgaggaacg ccaacaaacg
    62761 ctcgatttcc tcaaatcagg catcgccacg cgtaacccac acttcaaccg catgattgag
    62821 cagatcgaaa aagtggcgat caaatcccgc gcaccgatcc tgcttaacgg cccgaccggg
    62881 gcgggtaagt catttctggc gcgacgcatc tttgaattga aacaggcgcg gcatcagttt
    62941 agcggcgcgt ttgtggaaat gaactgcgcc accctgcgtg gcgataccgc catgtcgacg
    63001 ctgtttggtc atgtgaaagg cgcgtttacc ggggcgcggg agtcgcggga aggattatta
    63061 cgcagcgcca acggcgggat gttatttctt gatgagattg gcgaattggg cgcagacgaa
    63121 caggcaatgc tgctgaaagc cattgaagag aaaacctttt atccgtttgg cagcgatcgc
    63181 caggtgagta gcgactttca gcttatcgcc ggaacggtgc gcgatttgcg tcagctggtt
    63241 gccgaaggca aattccgcga agatctgtac gcgcggatca atctctggac cttcaccctg
    63301 ccgggtctac tccagcgcca ggaagatatt gaaccgaacc tggattatga agtggagcgc
    63361 cacgcctcac tcactggtga cagcgtgcgt tttaacaccg aagcgcggcg cgcctggctg
    63421 gcctttgcga catctcccca ggcaacatgg cgcggtaact ttcgcgaact ttctgccagc
    63481 gtcacgcgga tggccacctt tgccactagc ggacgcatca ctctggacgt ggttgaagat
    63541 gagataaacc gtctgcgcta taactggcag gagagccgtc cctccgcgct tacacaactg
    63601 ctgggcgcag aggcagaaaa catcgatctc ttcgaccgta tgcaactgga acacgttatc
    63661 gctatctgcc gccaggcaaa gtcgctttct gctgccggac gccagctttt tgacgtttcg
    63721 cgccagggca aagccagcgt caacgacgca gatcggctac gcaaatacct ggcgcgtttt
    63781 ggtctgacgt gggaagcctt gcaggatcag cacagctcca gttgaatatg atggtccgtc
    63841 agcacctgca tgacgcttac tggcggcggg gcgtcggtgt agacagcatc aaccatgctg
    63901 atgctcccca tattgaccat cgcattacgg ccaaatttcg agtgatcgac aaccagcata
    63961 acgtggcgcg agttctcaat aatagcgcgt ttggtgcgaa cttcgtggta atcgaattcc
    64021 agcaacgagc cgtcgctatc gatgccgctg atccccagaa tgccgaaatc aaggcggaac
    64081 tgggagataa aatcgagcgt cgcttcgcct atgatcccac catcgcggct acgtaattcg
    64141 ccaccggcga gaataatacg gaaatcttct tttaccatca acgtgttagc gacgttgaga
    64201 ttgttggtga caatgcgcaa attgctgtga ttaagcagcg cgtgcgccac cgcttccggg
    64261 gtggtgccga tatcgataaa cagcgtcgag ccgttgggga tttgctccgc cactttgcgg
    64321 gcgatgcgct ctttttcttc ggtctgggtg gccttgcgat cgtgccacgg ggtgttaacc
    64381 gaactggaag gcagcgccgc gccgccatga tggcgcagga tcaggttttg ctctgccagc
    64441 tcattgaggt cacgacgaat agtctgcggg ctgacggaga agtgctccac cagttcctcg
    64501 gtactgacat aaccttgctg tttaaccagt tcgataatac cgttgtgacg ttgtgtttgt
    64561 ttcatttata aatccctgga attattttcg ttttcgcgca ttgagcgaat caacaaaagc
    64621 catcgctaaa cccacggcta acccggcgat gtgtgctccg ttcgccatcg acatcccaaa
    64681 caaatcaaac catccggcga caatccagat cagcgcaaag ataattaacc cacgttgcag
    64741 gtaaatgcca ctttgcggat cgcgttcgcc acgtagccag atgtagccca tcagcgcata
    64801 caccacgcca gaaagcccgc caaaccacgg cccgctgaat ttttgctgca catagccgct
    64861 taacagggcg ctaatgagag taatgacaat tagcttaccg ctaccgaggc gtttttccac
    64921 cgcaccgccg agataccacc accagagcag gttaaagagg atatgcatca gcgagaagtg
    64981 cattaacgcg tgggtgaagt aacgccagaa ctcaaatttc agtgttggat cgaatggcca
    65041 ggccagccat aacatcactt cctgatcgcc gagaatttgc atggcaataa acaccaccac
    65101 gcaggcgatc atcatcaccc aggttaccgg acctgcgcgt tcacgcaagg cagcaaagaa
    65161 aggataacgg cgataatgca ggccactgcc ggtatggccc gcctgccagc tggccgccag
    65221 ataacgcgaa tctgccgggt tttcgagaaa acgcgccagc tccgcccgta cgcgctcggc
    65281 ctgggactca tccgccagcc agacgtcgct ttggttatgt tgttgaatcg tgaggataac
    65341 accctgcgtc gccatgtaat caacaaacgc ctgcgccacg cgggggttag caaaagaggt
    65401 aatcatcaac attgttgctg tcgcttattc cacacaaaag gggacagtat aaagcgttac
    65461 gcgccgtacg ccacctctgc gggaaactgt cgctgccagg cttcaaagcc gccgtcaatg
    65521 ctatagacca catcgtagcc ctgttgcagc agatactgcg ccgccccttt gctgctattg
    65581 ccgtgataac acatcaccat caccggagtg tcaaagtcgt tatcacgcat aaacgcgccc
    65641 agtgtgtcgt tggttaaatg gaaagcctgc accgcatgcc ccattgcgaa actctgtgga
    65701 tcgcgaatat cgaccagcac cgcctctttt tcctgcaact tctggtgcgc gtcggcaacg
    65761 ttaatacatt cgaactgatc catgcgtctc tctttcttta caaacaagtg ggcaaattta
    65821 ccgcacagtt tacgtcgaag cggcagataa acgccataat gttatacata tcactctaaa
    65881 atgttttttc aatgttacct aaagcgcgat tctttgctaa tatgttcgat aacgaacatt
    65941 tatgagcttt aacgaaagtg aatgagggca gcatggaaac caaagatctg attgtgatag
    66001 gtggcggcat caatggtgct ggtatcgcgg cagacgccgc tggacgcggt ttatccgtgc
    66061 tgatgctgga ggcgcaggat ctcgcttgtg cgacctcttc cgccagttca aaactcattc
    66121 acggtggcct gcgctacctt gagcactatg aattccgcct ggtcagcgag gcgctggctg
    66181 aacgtgaagt gctactgaaa atggccccgc atatcgcctt cccgatgcgt tttcgcctgc
    66241 cgcatcgtcc gcatctgcgc ccggcgtgga tgattcggat tggtctgttt atgtacgatc
    66301 atctgggtaa acgcaccagc ctaccgggat caaccggttt gcgttttggc gcaaattcag
    66361 tattaaaacc ggaaattaag cgcggattcg aatattccga ctgttgggta gatgacgccc
    66421 gtctggtgct cgccaacgcc cagatggtgg tacgtaaagg cggtgaagtg cttacccgga
    66481 ctcgtgccac ttctgctcgc cgcgaaaacg gcctgtggat tgtggaagca gaagatatcg
    66541 ataccggcaa aaaatatagc tggcaagcgc gcggcctcgt gaacgccacc ggcccgtggg
    66601 tgaagcagtt cttcgacgac gggatgcatc taccctcacc ttatggcatt cgcctgatca
    66661 aaggcagcca tattgtggtg ccgcgtgtgc atacacaaaa gcaggcctac attctgcaaa
    66721 acgaagataa acgtattgtc ttcgtgatcc cgtggatgga cgagttttcc atcatcggca
    66781 ccaccgatgt ggaatacaaa ggtgatccaa aagcagtaaa aattgaagag agtgaaatca
    66841 attacctgct gaaagtgtat aacacgcact ttaaaaagca gttaagccgg gacgatatcg
    66901 tctggaccta ctccggcgtg cgtccgctgt gtgatgatga gtccgattcg ccgcaggcta
    66961 tcactcgtga ttacaccctt gatatccatg atgaaaacgg caaagcgccg ctgctgtcgg
    67021 tattcggcgg taagttgacc acctaccgca aactggcgga acatgcgctg gaaaaactga
    67081 cgccgtatta tcagggcatt ggcccggcat ggacgaaaga gagcgtgctg ccgggtggtg
    67141 ccattgaagg cgaccgcgac gattactcag ctcgtctgcg ccgtcgttac ccattcctga
    67201 ccgaatcgct ggcacgtcac tacgcgcgca cttacggcag caacagcgag ctgctgctct
    67261 gcaatgcggg ggcgataggc gatctcgggg aagatttcgg tcatgagttc tacgaagcgg
    67321 aactgaaata cctggtggat cacgaatggg tccgccgtgc cgacgatgcc ctgtggcgtc
    67381 gcacaaaaca aggcatgtgg ctgaatgcgg atcaacaatc tcgtgtgagt cagtggctag
    67441 tggagtatac gcagcagagg ttatcgctgg cttcgtgatt taacgtaagg tggtcaggtc
    67501 agatttcaat ctgacctgag actgatgata aacaaaaaaa ctgcctgatg cgctacgctt
    67561 atcaggccta cgtggtttat gcaatatatt gaatttgcat gattttgtag acaaaataag
    67621 gcgttcacgc agcatccggc ataaacaaag cgcacttcgt caacaatctg acctactttt
    67681 tgattcaagg aattaccgca aagcccacgc aatcataccc aacgtaacaa aactcagaca
    67741 cattacccac tcgccccact tcttcctctg acttctattt ttgctacgcc actggcaata
    67801 atatcccaat gcaaaaccaa gctgggcgct tatcggaccg ataaagtcag tccacggctg
    67861 agacccaaat tgaaaaaatt caggggtaac cagacaatgg tcagaaagaa attgcgcata
    67921 ttttccagca aagcaatttg cgctggcgcg acatagaaaa tgatattcca caggagcaac
    67981 cagaaataaa ctggtgcaaa taccaaccaa caatggagtc tgcttttcag ttcagattga
    68041 gctctgcaag aaaattgcca accaacgcaa cacatggcac agaaaaaaaa agtgcatatt
    68101 cagccaataa cccaagttgg aattccagaa gtgaaatacg cctgaacatg agcgggatag
    68161 agaacagtat ttctcccata aaactcagcg tgataacaca agcagccagc gcaaccaccg
    68221 tcggccacac caacaacggc acaataacaa aaccaagaat taacggagga atataaagtc
    68281 tgattttatt catctttatt gtcctgatga ttcggatgat gcgtcataaa tcggcacata
    68341 gcccacatcc tctaccaatt gctgtccctg cgggcttaaa aaccagtcga cgaatttctg
    68401 cgtttccagc gtagggtttt cgtgcgtcac catataagca tgaattatgt acggataagt
    68461 gccgttacgg atattttcct ctgtaggtgc aacgccattt atagccagca gtttgatctg
    68521 tttgcgcatt ttgagcatat tgtcattcat acgtgtgacg taatagtgga aggtgtatcc
    68581 aatagaagat ggtgtatttt ggtagtcggc gacttcggta attaaaccgc ccataagatc
    68641 gataacagtg gattttttgg ctggcagcat tggagtatct tgcataacca gtcccttcat
    68701 aaccgtttgg cttccagaat cttctggccg ctgccagatt tttatacctt cgtcaccgcc
    68761 gcccacttta ttccagcgac tcactttacc gctaaaaatg tctttgacct gctgttcgct
    68821 aagtgaatta accgggtttt tgatatcaac gataaagaca aaggcttcgc gggcgaaagc
    68881 ggtatatttg agtttaacac ctgcttcttc tgcacgtttt ttctgtcctt ccgacggttg
    68941 tgcaacaaaa ataaccgtcg cggtaccctt aatcagacta tcgtaggctt cagctgttcg
    69001 ggtttgttgc aaaccatacg cctgccaggc cagcgaggtt cgatccttat cttttggctc
    69061 gggtactaat tcataatacg cggaggcata cagcgggtat aaggctgtag cgccgtacac
    69121 tctcggccat cttgaccaat catcgttgta acgtaattca ggttgccccc gcagagacgt
    69181 cagtccatgg ctgtcttctt ccctgacgct gttatagtac ccttcccggt cgacacatcc
    69241 cgccagtagc gccgtaccta atagtaaaac acaggtgaat ttattcattg cgctgattcc
    69301 tttctctcga gcgttatttc attgttggat acagcggcac atagcccaca tcttccacca
    69361 gactctgccc ctgcggcgtt aaaaaccatt caaccagttt ttgtgtttct gacgtcatat
    69421 tgtcccgcgt taccataaat gcatcgacga catagggata tttgccattg cgaatatttt
    69481 ctgcagtcgg tgcaataccg ttaatcgcta gcagttttat atttttatcg gcattcattt
    69541 gtgttgcgta atagcggaag gtgtagccta ttgcgttgtt tgtattacgg tattcggcaa
    69601 cgactttaat cattccctcc atcatgcttg ccacttcggt ttcctgcggc gagatcatgc
    69661 ggacattttt catgacctgc gattgcatca ctgtctggct gccagagtct tccgggcgtt
    69721 gccaggtctg gatctcctga tcgttaccgc caaccgtgcg ccagttggta attgcgccac
    69781 taaaaatgtc acgcacttgt tgttcagtca gggaattaac cggattatcc gcattgacga
    69841 tgaaaacaaa cgcttcacgg gcaaatggcg tgtacagcaa agtgacgccc gatttctcag
    69901 cgcgtttttt ttgcccatcg gaaggttgcg ccacgaaaat aatatcggca tcaccgttaa
    69961 cgattttgtt atatgcttct tgcgtacgag agttatccag ataatcccaa acgtgaaaat
    70021 cctctggtat tacacttaat gcataaaatg cagaggcata aatggggtac gccgccgtag
    70081 cgccatcgag tcgcggccag ttttgcgtaa attgaatttg tggtttacca cgcagtggcg
    70141 tcagttgatt attaagttta tcgggtcgcc aggcccaggt atcaatattt tcacgcacac
    70201 cgacgccggg attaagctgt ttatataaat aagcctgata accagcgaca atacccagta
    70261 agagaagaat gaacagaagt aaattacggc aacgtagacc ggaggtgccg gttaaagggt
    70321 gtctggtcgg ccagccgtat cccagtgaaa aactgatatg cgcgccaacg ggaataatga
    70381 ctatccacca gtactcacca ccgacgaatg agactgtaaa aacgcccaac cagggaagca
    70441 taaggtttgc caaccaaccg gctcccatct ggcgaaattg ataatctgcc agatgtagcg
    70501 tgattatcca gccaaccagg gttaataaag cgggaactaa aaaaagacat tgtcgccagg
    70561 cggctatttc tctgttttct ggtgcgctat cgcccgccca cactcgccca gagaaaaata
    70621 gcagtaatgc gcccagcgtc agcataaaga cgttgagtag aagataaatt gttggtgtaa
    70681 agagtaaatt gcaaaactca agaatagcgg caagcccggc ccccagcatg gcaacaaccg
    70741 ctgccaaaaa ttgcgccagt atggggatgc caaaaaaagt cattaccagc gaggtcaaaa
    70801 tccattttcc gttttgcatt attctttcca ttcttgctga atggcgaaat tatactcccc
    70861 gataaccctc gccccctctg gacacttttc ctaaataaag gcgaaaaaaa acgggacctt
    70921 ttggccccgt tctgtttttt agcgaactta caatctcacc ggatcaatat gccagatatg
    70981 atcggcgtac tctttgatcg tacggtcaga agagaagtag cccatattgg caatgttcag
    71041 catcgctttt gcggtccact cttcctgacg ctcgtagagt tcgtcgactt tatcctgaca
    71101 atcgacatag ctgcgataat ccgccagtac ctggtagtga tcgccgaagt tgatcagcga
    71161 atcgaccaga tcgcgatagc gacccggatc ttccggactg aatacaccgc tgccgatttg
    71221 cgtcagcacc tgatgcagct cctcatcttt ctcgtagtat tcacgcggtt tgtagccctg
    71281 acgacgcagt tcttccactt cttccgctgt gttaccaaag ataaagatat tgtcagcacc
    71341 gacatgatcc agcatctcga cattcgcacc gtccagcgta ccgatagtca gcgcgccgtt
    71401 aagcgcaaac ttcatgttac tggtgccgga agcttccgtc cccgccagcg aaatctgttc
    71461 agatagatcg gccgccggaa tgatcaactg cgccaggcta acactgtagt tcgggatgaa
    71521 cacgactttc agcttgtcgc caatctgcgg atcgttgttg atcactttcg ctacgtcatt
    71581 gatcaaatga ataatgtgct tcgccatgta ataggccgaa gccgccttac cgccaaaaat
    71641 attcacgcgc ggtacccact tcgcatccgg gtcggccttg atgcggttat agcgggtgat
    71701 cacgtgcaac acattcatca attgacgttt gtattcgtga atacgtttaa tttgcacatc
    71761 gaacagcgct ttcggattta ccaccacatt cagctgctgg gcgatatact ccgccagacg
    71821 ctttttgttc tccagcttcg cctgatgcac cgcgtgatta accattggga aatcacagtg
    71881 ttgttgcagc tcattaagca ggctaaggtc ggtgcgccag ttacggccca ggtgttcgtc
    71941 cagcacagct gaaagcgatg ggttcgctac cgccagccag cgacgcgggg tcacaccgtt
    72001 ggtaacgttg gtgaaacgac ccgggaagat tttcgcaaag tcggcaaaca acgattgcac
    72061 catcaggtta gagtgcagtt ccgatacacc gttaactttg tggctcacca caaccgccag
    72121 ccaggccata cgcacacgac gaccgttgga ttcatcaatg atcgacgccc gtcccagcag
    72181 atcggtatcg ttcggatact gttcctgcaa ggttttaagg aaatagtcgt tgatttcaaa
    72241 gatgatctgc aggtgacgcg gcagaatttt acccagcata tcgactggcc aggtttccag
    72301 cgcctcgctc attagcgtgt ggttggtgta ggagaagacc tgacaacaca cttcaaacgc
    72361 gtcgtcccag ctaaattggt gctcatcgat cagcagacgc atcatctcag gaatcgacag
    72421 caccggatgg gtatcgttga ggtgaatcgc aattttatcc gccaggttat cgtaggtttt
    72481 atgcaactga taatggcggc ttaaaatgtc ctgaatggtc gaggaaacca ggaagtattc
    72541 ctgacgcagt cgcagctcac gcccggagta ggtggagtcg tccggataca gtacgcgaga
    72601 tacgttctcg gagtggtttt tatcttccac tgccgcgaag tagtcgccct ggttgaattt
    72661 accgaggtta atttcgctac tggcttgggc actccacaaa cgcaacgtgt tggtcgcgtc
    72721 ggtgtcgtaa cctgggatta tctgatcgta agctacgccc agaatctctt cggtttcaat
    72781 ccagcgcgtt tttttacctt cctgctgaat gcgaccgcca aaacggactt tatagcgcgt
    72841 gttgtggcgt ttgaattccc acgggttacc gtattccagc cagtagtcag gcgactcttt
    72901 ctggctaccg ttaacgatgt tctgcttgaa cataccgtag tcatagcgga tgccgtaacc
    72961 gcgccccggc aaccctaacg tcgccagaga atcaaggaag caagccgcca gacgtcccag
    73021 gccaccgtta ccgaggcccg ggtcattttc ttcatcaatc agctcttcga gatttaaccc
    73081 catcgcttcc agtgcgccct gtacatcttc gtaaattcct agcgacaaca tggcgttgga
    73141 aagcgtacgg ccaatcaaaa actccatcga caggtagtaa acctgacgag tttcttgcga
    73201 caactgggca cggtttgaac gtaaccagcg ctccacgaga cgatcgcgca cagcaaataa
    73261 cgttgcgttc agccattcat gtttattggc gacgaccggg tcctttccaa tcgtaaacat
    73321 cagcttgtaa gcgatagagt gcttaagagc ttctacgcta agcgtgggcg atgaatatgt
    73381 aaacggagca ttcatatagg cgtttcctga aaactatttc aagcgatagt aaagctcacg
    73441 gtacgacttc gccgcgacct gccagctaaa atccattgcc atagcctgac gttgcacaaa
    73501 ccgccacagt gaaggacgag accacagtac aaaagcacgt cgaatagccc gtaacagcga
    73561 ccaggcatta ctatcttcaa agacaaaccc actggcgacg ccatctgcaa ggttctcaag
    73621 agaacaatca gaaaccgtat cagcaagccc accggtgcgc cgcactaacg gcagcgtacc
    73681 gtacttcaat ccataaagtt gcgttaagcc gcacggttca aaacgactgg gcaccagaat
    73741 gacgtccgcg ccgcccataa tgcgatgcga aaatgcttcg tgatagccaa tctgaacgcc
    73801 cacctggccg gggtattccg ctgccgccgc aaggaaacct tcctgcagca ccggatcgcc
    73861 cgcgccgagt agcgccagtt gcccgccctg ctccagaaga cccggtaagg cctccagcac
    73921 caggtcgaga cctttctggc tggtcagacg gctcaccact gcaaaaagcg gcactttatc
    73981 gtcaacctta agccccattg cgatttgtaa ctggcgctta ttttccgctt tatcttccaa
    74041 cgtatcgcgg gtgtaacgcg aggccaacag taagtccgtc tctggactcc agattttctc
    74101 gtccacgccg ttcagtacgc cggaaagacg cccttcacgg tgacgctgtt gcaacagacc
    74161 ttccataccg taggcaaact gcggttcggt gatctcgcga gcgtaggttg ggctgactgc
    74221 cgtaatgtga tcggcatagt acagaccggc cttcaggaaa gagatttgtc cgttgaattc
    74281 cagcccatga atattaaaga acgaccatgg caattggatg tcattcatgt gatgtgcata
    74341 aaacatgcct tgataggcca ggttgtgcac agtaaacacc gacttcgccg gacgcccgcg
    74401 cgccgccaga tacgcaggcg caaggcctgc atgccagtcg tgcgcatgca ccacatcagg
    74461 acgccagaat gggtcaagcc cgctggccat ttctgcccca acccacccca gcagcgcaaa
    74521 acgcaacacg ttgtcggtat aggcaaataa gttggtatcg tgatacgggc ttcccggacg
    74581 atcatagaga tgcggcgcgt caatcaggta aatgccaacc ccgttgtaat gaccgaacaa
    74641 cagcgtgata tgtccggcga aggtatcacg acgggatact acctgcgcat cggtcacgcc
    74701 acggcgaata tcgggaaatg caggcaacag tacgcgagcg tcaacgccgt ctgcgatttg
    74761 tgctgcgggt aatgccccaa taacatcagc cagaccgccg gttttaagca gcgggaacat
    74821 ctctgaacat acatgtaaaa cctgcattat cgctcctgtt tatgccctaa cttccgtagc
    74881 atttcgcgcg ttaccagcac gatgccttct tctgaacgat agaaacgacg tgcatcttcc
    74941 tctgcgtttt caccaatcac catgccttcc ggaataacac aagcacgatc gatgacgcag
    75001 cggcgcagac ggcacgagcg acctacccat acttccggta acaatacggc ggaatcaatg
    75061 ttgcagaatg aattcacgcg aacgcgcgag aacagaacgg actgcaccac caccgaaccg
    75121 gagatcacac aaccgccgga taccagtgag ttaagggtca tcccgtggct accggagcga
    75181 tcctgcacga atttcgctgg cggtaatgat tcattgtagg tgcgaattgg ccaattgcga
    75241 tcgtacatat ccagttccgg caccacagag gccagatcga ggttcgcttt ccagtaagct
    75301 tccagcgtac ccacatcgcg ccagtacggc tcggcatccg ggtcggattg tacgcaagag
    75361 agcgggaatg ggtgtgcata ggccagaccg gcttcggtga tcttgggaat caaatctttg
    75421 ccaaagtcgt ggctggagtt ctcatcgcga tcgtcttctt ccagcagttc atacagatag
    75481 tcggcgtcaa agacgtagat acccatactc gccagagatt tgctcggatc gttcggcatt
    75541 gacggcgggt tagctggttt ttcaacgaat tcgataattt tatcgttctc atcaaccgcc
    75601 ataacgccaa atgcggaggc ttcttcaatc ggtactggca tacaagcaac ggtgcaacgt
    75661 gcgccttttt cgacgtggtc gataagcata cgcgagtagt cttgcttgta gatatggtcg
    75721 cccgccagga tcaccacgta ttccgcttta taacggcgga taatgtcgag gttttgggta
    75781 actgcatctg cggtgccgcg ataccagttt tcccctttca ttctctgctg tgctggcagc
    75841 agatcgacaa actcgttcat ttcttcattg aagaatgacc agccgcgctg aatgtgctgc
    75901 accagagtgt gggactggta ctgggtgatc acgcccatac gacggatccc ggagttgatg
    75961 cagttagaca gcgcaaagtc gataatgcgg aacttaccgc cgaagtgtac ggccggtttt
    76021 gctcgcttat tggttaaatc cttcaggcgg gtaccacgtc ctcccgccag tatcagggca
    76081 acagatttca atggcagctg gcgcgccaac attaagtgat cgttcttctc taaactaacc
    76141 atgactaact ccttttttat catctctgga acacacacaa tccgtgtgca ggtccctgcc
    76201 agacagccgt aatcactggg ttatcctctc cagcgaatgg gggaatggcg tgccactccc
    76261 cagcaggtaa aacaatctct gttacctcaa gcgtggcgtt aattgcgatc aaaaagcgat
    76321 ccgagagcag aatttgcagc tgtttcggcc cgttttgcca ctcatccgtg cttaaaggtt
    76381 gagcatatcg atttagccaa cggacattgc cgtcgccttc ttcccaccag cgattctcca
    76441 ccaaagcggg aatgcgcttg cgcagatgga ttaacgcggc ggtaaatgcg gttaaaccac
    76501 tgcttgcctg cgaccagtcc aaccaggtta attggttatc ctgacagtag gcattgttat
    76561 tgccatgctg gctgtgacca tgttcgtcac cggccagtaa catcggcgta ccctgggaga
    76621 gcaacaacgt tgttaacagg gcgtgaatgc tgtcgcgacg ccgttcaacc aggtcaagag
    76681 taccgcctaa cccttcttta ccatgattgt tactataatt gttgttggtc ccgtcgcgat
    76741 tttcttctcc gtttgcttca ttatgtttat ggttgaagca aacgcagtcg cgaagcgtaa
    76801 aaccgtcatg cgcggtgacg agattaatcg cggcactcgg cagacgacca ttacgtttaa
    76861 aaacatcgct ggaggcagca aaacgcccgg caaacgcccc cagaggcaaa tcataatgaa
    76921 gccagaaacg acgggcagca tcgcggaaat gatcgttcca ctcggcaaac agcggcggga
    76981 aatttcccac ctgataacca ccaggagcaa tatcccacgg ttcagcaatt aacttcacct
    77041 gcgagagcac cgggcagttc tggatagcgg taaacaacgg cgcatcctga cggaactctg
    77101 gcgtacggcc catgactgcc gccagatcaa agcggaaacc atcgacgtgg caggtttcta
    77161 cccaataacg caggcaagcg ctggcataat ccactaccgc cggatgactc aaattgagcg
    77221 tgttgccaca accggtccag ttgtgataat cgccgtcttc tcttatccaa taatagctac
    77281 ggttgtcgat cccacgcaga gagaataacg gaccgtcgag gtccagttcc gcactatggt
    77341 tgagcacgat atcaagaatg acttcgatac ccgctttatg cagtgctttg attgcatcgc
    77401 gaaactcatc cagcgccgtt tctggggagc aggcatacgc cggatgcagc gcaaacatcg
    77461 ccaccgggtt ataaccccag tagttactta gacccatacg ttgcaggcgt ggttcgctgg
    77521 caaactgcgc caccggcagc agttccagcg cggtaatgcc caactgtttc aaatagttga
    77581 tcatcaccgg atgccccagg gctttataag tgccacggat ctcgaccggg atctccggat
    77641 gcaggtacgt taatcctttg acatgggctt cataaatgat ggtgctgccc cacggcgtgc
    77701 gcggcggggc atcatcttcc cagtcatagt gatcaaccac cactacgcat ttcggcgcaa
    77761 tggcggcgtt gtcgcgatag tcaggttcat tatgaccggc gtgcagcagc gggttatctt
    77821 taaattcccc gtcaatttgc cgcgcgcaag gatcaatcaa caacttcgcc gggttaaagc
    77881 gatgcccctc ggcgggttgc caggggccat gaacgcgata accataacgc aaacccggac
    77941 gcgcatccgg cagataaccg tgccaaatgt cgccactgtg ccctggcaag tcatagcgat
    78001 gttcctggcc attggcgtca aagatacaca gttctacccg ctcggcatga gcggagaaaa
    78061 gtgtgaagtt gacgccctga ccgtcgtaat gcgcgccgag gggtgcgggt ttgccaatgg
    78121 cgagttgtgt cattctgcct cccgaaccag ccaaatagtg gccagcggtg gtagtgtcag
    78181 gcttaatgaa tgctgacgac cgtggctggc aatctcatcg ctgtgtaccg tgccgccatt
    78241 gccgacatta ctgccgtgat agtgcatgga atcggtatta aggatttcac gccatttgcc
    78301 cggctggtta atgccgaagc gataatcatg acgcggcacc ggagtaaagt tactggcaac
    78361 gatgatttcg ttaccctctt tatcgcgacg cacaaagatc agcaccgagc gttctttgtc
    78421 atccaccacc agccattcaa agccgtacgg gtcaaaatcc agttcatgca ttgctttatg
    78481 gtggcggtag gtgaggttca gatcgcgcac cagacgctgg acaccgtggt gccagttatc
    78541 gccgccttcc aacagatgcc agtcgaggct ggcgtcatgg ttccactcgc ggccctgggc
    78601 aaattcgttc cccatgaaca gcagtttctt gcccgggaat gcccacatcc agccgtagta
    78661 ggcgcgcagg ttagcgaatt tctgccatgc gtcgcccggc atccggtcga gaatcgattt
    78721 tttaccgtgg accacttcat catgcgacaa cggcaggacg aagttttcag tgtagttgta
    78781 gagcatcccg aaggtcagtt tatcgtgatg atactgacga taaaccgggt cgagcttcat
    78841 gtagtccagg gtgtcatgca tccatcccag gttccacttg taccagaagc ccagaccgcc
    78901 catgtcctgc ggacgagaaa cgccagggaa atcggtagac tcctccgcca ttgtcaccgc
    78961 accggaaacc tgctcaccaa gaatacggtt ggtattacgc aagaattcaa tcgcttcaag
    79021 attctcgcgc ccgccaaatt cgttcgggat ccactccccc tctttacggc tgtagtcgcg
    79081 ataaatcatt gacgccaccg catcgacgcg cagcgcatca ataccaaaac gttcaatcca
    79141 gtaaagcgcg ttaccgacaa ggaagttact gacttcacgg cgaccatagt tgtagatcag
    79201 cgtattccag tcctgatgat agccttcgcg cggatcgctg tgttcataca agttcgtgcc
    79261 atcaaattcg gcaagcgcaa agtcatcggt cgggaagtgg cctggcaccc agtcgagaat
    79321 cacgttcaga ccagctgcgt gtgcggcatc aatgaaataa cggaagtcgt cgcgagtacc
    79381 aaaacggcgg gtcggtgcat acaggccggt tggctgataa ccccaactgc catcgaaggg
    79441 atgctcgtta atgggcagca gttcgaggtg ggtaaagccc atccatttag cataaggcac
    79501 cagttgatcg gccagctcgc ggtagctcaa ccagaaattg ttgtcggtgt ggcgacgcca
    79561 ggagcccagg tgaacttcat aaatagagat tggcgcatca aactgattcg cttttttgcg
    79621 ctcttcagtc tgcacaacct tttccggcag accgcaaata agagacgcgg tttccgggcg
    79681 catttgcgct tcgaaggcat aagggtcgga cttcagacgc aagttgccat tggcatcaat
    79741 catctcatat ttatagagct gaccgttatg cgccccaggg ataaacagtt cccagatgcc
    79801 gctctcttta cgcaggcgca tcggatgacg gcgaccgtcc cagtagttga attgcccaac
    79861 caccgagacc cgacgggcgt ttggagccca gacagaaaaa cgcgtacctg tgacgccatc
    79921 catagtatct gcatgcgcgc ctaaggtttc atacgggcgc aggtgagtac cttcagataa
    79981 tagccaggca tccatttcct ggattagcgg accaaaacgg taaggatcat ctatcaggtt
    80041 ttgctgacca tgccagacaa cagccaactg atagcggaaa aaattcttac gtcgcggaat
    80101 gacaccgcta aagaatccac gggagtcgag acactccagt tttgcgagtt tgcgcccggt
    80161 tttcggttca atcacccaca catcggtagc gtcgggtaaa agggcacgga cttccagtcc
    80221 cgcggtggtt ttatgcatcc ccagtacgga aaaaggatcc gcaaaatggc ctgcaattag
    80281 cgcgttaatc acgtctctat cgatacgatc ggacatgctt gtcttcctgt tttattgtgt
    80341 caccccatcc aactggattt ttgacttctg gttgtgacat ttttttgacc tgaacggcgc
    80401 agcactctgt gcatcctctc tgcgtcgtcc tcacttcagg taaggctgtg aatactcatg
    80461 tattcagcca cccttaaaga atagccaatg ctctatttaa ctcccggtaa atcatgaaac
    80521 atctgcgctt actcctgtat tacgcactag caggggcggc atcgcgcccc agatttaatg
    80581 aataaagatt acgccagttg acgaagcatc cgacgcagcg gctccgcggc cccccacagc
    80641 agctggtcgc ccacggtaaa ggctgacagg aactctggtc ccatattcag cttacgcagg
    80701 cggcctaccg gcgtggtcag cgtgccggta acggcagctg gggttagctc acgcatagtg
    80761 atttcccgat cgttcggaac gactttcgcc cacggattgt gcgcagccag cagttcttcc
    80821 acggtcggaa tagacacatc ttttttcaat ttaatagtga atgcctggct gtggcagcgc
    80881 aatgccccga cacgcacaca taaaccatct accggaatta cggaagatgt gttgaggatc
    80941 ttgttggttt ccgcctgccc tttccactct tcgcggctct gaccgttatc aagctgtttg
    81001 tcgatccacg gaatcaggct acccgccagc ggcacgccaa agttatccac cggcagctca
    81061 ccgctacggg ttaaggttgt gactttgcgt tcgatatcga gaatagcaga ggacggggtc
    81121 gcgagttcat ctgccacatg gctatacaga tggcccatct gggttaataa ctcacgcata
    81181 tgtcgcgcac caccgccgga agcggcctgg taggttgcaa cggacaccca atcaacaaga
    81241 tcattggcga ataaaccacc caacgacatc aacatcaggc ttacggtaca gttaccgcca
    81301 acaaaagtcc tgatgccatt atttaatccg tcggtaatga cgtcctgatt gacggggtca
    81361 agaatgatga tggcgtcatc tttcatgcgc agagacgatg ctgcgtcaat ccagtaacct
    81421 tgccatccgc tttcacgaag ctttggatag atttcgttgg tataatcgcc gccctgacag
    81481 gtcacaatga tatcgagggc ctttagcgcc tccagatcaa aggcatcctg aagtgtgcca
    81541 gtggttccgc caaaagacgg cgcagcctga ccaagctgag aagtagaaaa gaagacaggg
    81601 cgaatggcgt cgaagtcgcg ctcttcaacc atgcgttgca tgagaacgga gccgaccata
    81661 ccgcgccagc cgataaaacc aacatttttc ataagcgttt tttcctgcaa agatgtgtgc
    81721 tgtataaatg tgccggtctc ctcttggcac atctttcacc atacaaaaag cagccaaagt
    81781 cgcaagtgaa attaatcaat gatagcgaag ccatcagtaa tgcgacttat cctgctttgt
    81841 tagcacgcag aaagtccgcg gcaattatca gggaatttga gttatgaatg aaatcatttc
    81901 tgcagcagtt ttattgatcc tgattatgga tccgctcgga aacctaccta ttttcatgtc
    81961 cgtactgaaa catactgaac cgaaaagacg gcgggcaatc atggtgcgag agttgcttat
    82021 tgctctcctg gtgatgctgg tgttcctgtt tgcgggtgag aaaattctgg catttcttag
    82081 cctgcgggca gaaaccgtct ccatttctgg cggcatcatt ctgtttctga tcgccattaa
    82141 gatgattttc cccagtgctt caggaaatag cagcgggctt ccggcaggtg aagagccgtt
    82201 tatcgtgccg ttggcaattc cgttagtcgc cgggccgact attctcgcca cgctgatgtt
    82261 gttgtctcat cagtacccga atcagatggg acatttggta attgctctgc tgctggcctg
    82321 gggtggcacc tttgtcatcc tgctacagtc ttcgctattt ttacgtctgc tgggcgagaa
    82381 aggggtgaac gcacttgaac gcctgatggg attgattctg gtgatgatgg caacccagat
    82441 gttcctcgac ggcattcgaa tgtggatgaa ggggtaaggt tcaaacaggt gctgcacaca
    82501 accagaggcg gcacctgttt tattacctga actgataatg accttcgccc aatcggataa
    82561 cccttccctc ttcttctgcg cggttcagcc acatactaat ttgttcgatg gttaaaccag
    82621 tacaagacgc cagcctttca atacttatcg attcctttgc aaggatcgcc agttccgcca
    82681 caaaaagttg ataaaaatcg acaggggtat gcgagggcat cactgcgtaa tttgcgtcat
    82741 cttcgtgcag cgaaaacaat tctggttgag cgttacggag tgctctgtca tacatcagcc
    82801 caggaacgtc tggcagtctg atcgtcacat tctcagcctg ttcagcactc catgacgccc
    82861 cattttctac taatcgtgaa ttggctgatt gcacatcctg atttggttta accagaacag
    82921 gcagccactg atgttttaac gcctccatcg ctccggttat cgttccaccg gtcattcccg
    82981 cacgtacaac cattgcgctt tcagcaaggc aataaatata tttattccgc gccatcgcat
    83041 tgccgacggt gaaacgtacc tctgggtaaa acggtgaaat caacaccagg ttgcctgcta
    83101 taagcccttc acgccatttc actaacgtgc tcgtttttaa caagctatca gctaatacgc
    83161 caacggcagt tcctccggcc tccagcgccg acgctattgc acattcatca atacctcgcg
    83221 caccaccaga gataacgcaa atcccctgtt gggccagttt cgctgccagt tgttgggtat
    83281 agcgcaaatc ggcagtcgga gcatctcgcg agccaacaat cgccatacct tccgcttgca
    83341 gtaatgcttt attcccgcag ccaaataaaa cgggaggtgc atccgttcgc aatcggtttt
    83401 tcaagcgaac gggataatcg gcatctccgc gggttaagat ccataaaccg gcgcgttgcc
    83461 acttatctac cgccaacgcc agactatgac cacgcgccag caagccaaga atacgctcct
    83521 gagaaatacg cggatcgctc cagcctgtaa gtttctcttg cggttgcggc accagaagat
    83581 cggcgggact gatacgttgg tgcttcagcc ataatgcaaa cttcccccat tcactattac
    83641 taagaggttt atactcactc gccgccgcgc gagaaaaatc gctggttagc aggagagtcg
    83701 cttgtgcatt ggctgaaaga ttcatcaatt ttttaccgag gtagaagcaa gggcaagagg
    83761 ataaaccgtc gggcaacctg cctggcgtaa cagtgctgtc agaactgtca gcgtccatgc
    83821 agaatccacg atatcgtcaa ccagcaacgc cggacctggc atcaaaggag gggtaatcac
    83881 aaacgccccg tcgagatttt gacactggtg aaaacggttt tgctgcattt tctgcggtgg
    83941 attgtccacg actttttcaa tggcatcaat aaaaggtaag ccaagtttcg ccgccagtcg
    84001 ccgggcaaaa tcaggaacca ggtcgaggtg ccttaatgaa ggaacgcagc atacccaggt
    84061 tggttcagga tgcggattcc agcgttggcg aaccatttcc gcacatgctt ctaccagctc
    84121 atcacttaag cgccccgttt ttttctcctg tgctacctgc tttccccacc cggaatctcc
    84181 ccaacgagaa aggattcgcc ccgtagatcc ttgtaaaccc gcaggcaagt tccctttaaa
    84241 tccgtattgc gtaaaagccc cagctgccac ctgtttattg agatttaacg gcaggtcagc
    84301 gtgtttaata aataaatttg ccgcatggag taagccgcta tcaatgtcgg gcgacaataa
    84361 cggatgctga agacaactgc tacatttacc acaacgttcg gcacttggct catcgagtgc
    84421 acggcgcaga aattgcattt tgcactcctt ggtcgtgata tagctttcaa cgtctgccag
    84481 ttcacttttt ctctgattca tcaaatggtt aattcgttca tgatcaggag aaaaactgac
    84541 gatagttctg cgccataatt tctcggtata caccacaggc gatggatttt ccgctaccaa
    84601 taatttcagt gctttttcta tttgcccata acgaagattc gtccgttgtt caatgcctcg
    84661 tagggtaaga ccgtcattct cgctaagtac gttgagtatt tcatgaattt gcgcctccgc
    84721 aggaaaggcg ctttcacgga agaattgatg aatagcgcgg tcttcaccac cacaaagcaa
    84781 tatgccaact gcggaatcta tagcacgccc ggcacgcccc acctgttggt agtaaccgac
    84841 aatagacccc ggcatctgat aatgaatgac gaaacctaaa tcaggtttat caaaccccat
    84901 acctaatgcc gtcgtcgcga ccagcacttt gattttattt gccagcagtg cctgctccag
    84961 atattccctg gcagtattag aatcttccgc gccttcacaa gtcacgccgc tgtagtaagc
    85021 aaatgcactg ataccatttt tccttaacca ctcggcgaca agttcagcat cgcgagtcgt
    85081 cagggtataa acaattcccg atttggaaaa ctgagggata accgttgcta accatgccag
    85141 acgggatgac tgttctccta agactaaggc atcaagcgcc aaactttcgc gagccagcgt
    85201 tccacgctga atcacaatgt cacccaattg ctgacggata tcctcaacaa cacggttatt
    85261 cgctgtcgcg gttgtaccca gaacaggggt attcgcaggc agttggcgca aaatatctaa
    85321 tatacgtcgg taatccgggc gaaaatcgtg gccccaatca gagatgcagt gcgcctcgtc
    85381 gaccacaagc aagccaatac gatcggctat aggatataaa accgtttcga taaagtcctg
    85441 gtttgccaaa cgttccgggg aaaccagcaa gcaatcgacc tcaccttgca gcaacttatc
    85501 gctaatgcgc tgccattctt ccctgtttgt agaattcaac gtttcagccg taatacctaa
    85561 ccgttcagcg gcggcaacct ggttacgcat cagggcaagc aaaggagaga tgataattgt
    85621 cgggccagcg ccgcgatcgc ggaagatttt actggcgatg aagtacacgg cacttttccc
    85681 ccagccggtg cgctgtacaa ccagcagttt tcgccgctga ttaaccactg catctatggc
    85741 ttcccattgc ccttccctga aagtagcggt ggtatttcct aacatccgct gtaaaagcaa
    85801 ttcagctccg tgcttttcca tctttatctc ctgcgatctt taggcagtga ttagaacgga
    85861 ttattagcag gtaaatttgc gagagagatc aaaaagatga agagaggaga taagagaaat
    85921 gaattactgc gagcaagctc gcagtaattt gtcgcagtaa ttaactcaac agctggaacg
    85981 caatcatccc aacgatggca ccaacagtgc cgagtatggt ttccatcatg gtccaggttt
    86041 tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa caaccagaaa ccggcgtcgt
    86101 taacgtggct gacaacaatc gaaccaccgg cgatacaaat cgacagcgcc gccatttgcg
    86161 caccggagta gttcagttgt tcaataaccg gcatcaccag tcccaccgcc gttaaacagg
    86221 caacggtggc agaaccctga atgatgcgca ctgcagctgc cagcacgaag caggtgatgg
    86281 caatcggcag gcccatgccg gttaacgctt cgcccagtgc cggacctacg ccagagtcaa
    86341 ccagcacctg tttgaacacg ccgcctgcac caatcaccag cagaatgatc cccgccggtt
    86401 gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg catgccctga cgcattgcca
    86461 ggccataaat cgccaccaga caagcaacca gaatcgcggt aaacggatga ccaataaact
    86521 cgaaccattc gtaagcggta gagccttctg gcacaaaacg cgcggcaatg gttttcagcc
    86581 ctaccagcac cagcggcaac aggatcagcg acaggctgaa tccgaaagat ggcattttgc
    86641 cttcgccgag atgtggttcg ctgatgtcgt caggaatatg cagctccacg tagcggctga
    86701 tgaaattacc ccacagcggc ccggcaataa tcattcccgg aattgccgca cacaggccaa
    86761 tcaggatcat ccagccaaag tcggcattca tctgcgatgc cagcagcatt ggcgctggtc
    86821 caggcaccag gaacgcagcc gcggccgcca cgcctgcgaa taatgggatt accagcttca
    86881 ccaggttcgt accggtgtgg cgcgccattg agaaagcaac gctaatcagc agaacaatcg
    86941 ccacttcaaa gaacagcggt agcgcacaga ccagccccgc aaggccgatg gcataatgcg
    87001 cgcggctgtg accgaaggat ttgagcattt tgacggcaat ctgatcgact gcgccggttt
    87061 catgtaagat cttgccaaac atagctccca gggcgacaac caccgccagg aagccgaggg
    87121 tgcctcccat ccctttttcc atcgtcgctg cgattttatc gagcggcata ccggaaaaaa
    87181 ggccagcccc catggacacc accatcaaag ccaggaaagc gtgcatacgc gccttcatga
    87241 ctaaaaacag cagcagtaaa acagacccta ctgctgttaa aacaagcgtt aatgtagtca
    87301 ctacttattt gcctttttta ataacctcaa tggtgcttgc cacaacacct tccagcggtt
    87361 gatcgatatc caccaccagt acatcggttt cgtccgcacc cggctcctgc agcgtttcaa
    87421 actgcgtcac caacatttgg gttttaaaga aatggccttt gcgcgctttc aggcggcttt
    87481 caatcacatc aaaatcgcct ttcaggtaga tgaacgagag attcggatta ccttcacgca
    87541 gcaagtcgcg atagtgtttt ttcagtgcag aacagacgat cagcgacact ttattggtgc
    87601 gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca cggtttgcga tcgtcgtcat
    87661 tcagcggttc accagacgcc attttttcga tattgcgcct tggatggagg aaatcgccat
    87721 caagaaacgc ggcatgaagt tgatgcgcca cttcactggc gaccgcagat ttgccgctgc
    87781 ccgatacgcc catcaagacg taaatgtggt gatcatggtt agtcgtgctc aaagtggtgc
    87841 ccccacaata caagaaataa aaatgttacg ggtaactgtt atcggtaaca ttgtccggcc
    87901 ggacaatatc agaagcaata tccatccgtg ccttaagtgt ataagtgtga gctacttcaa
    87961 atttgtgggc ttaaatagat ccgcccggtg acaaggtgaa acctaaatct aacattttcg
    88021 gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc agcgccaata ctgcccatcc
    88081 gctcacgcgg cgtcagtacg ctcgccaacc gtggttccat cacctgacca atgtcatgac
    88141 catggaaacc ggcaatcgcc atatcgtcag gaactttcaa ccccagacgc tggcattcaa
    88201 acgccgcgcc gaccgccagg tcatcattag tacagaacac gccatccagc tgcggatatt
    88261 cccgcctcgc ctggcgaatc agttcaatac cggaagagta agaagaagat tgctcaacca
    88321 tcacgctata cggcaccagg cctgcatcca gcatcgcctg ttcgtatccc ttctgtttga
    88381 tgatagtacg ttcgtcgaga cgtgcgccga gataggcaat gtggcgatgc ccgcgagcaa
    88441 taatggcggt ggtcatctgg cgtgccgctt caaagttatc aaaaccgacg gcaatatcga
    88501 ggcacggcga cttgctgtcc atcaactcca ccaccggaat ccccgccact tcaatcatct
    88561 ttaaggtgcg tggcgtgtgg gtacgttcgg tgaggatcag tccatcgata ttccatgaga
    88621 gcattgattc gaggcgttct tgctccattt ccggtttata accgtagtgc gccagcatag
    88681 tttgataacc gtgcgcgtcg gtgacgcttt cgattccgcg taatacttcc gcgaaaacct
    88741 ggttggtgag agaaggtaac aggacgccaa tcgcccggct ggtggcatta gagaggatat
    88801 cgggcgcgcg attgggaata taacccagtt catcaagagc ggcggcaatc ttgccgcgta
    88861 gagcgacgga aacctgctcc gggttgcgta aaaaacggct gaccgtcatt ttggtcacgc
    88921 ctacacggtc agccacatcc tgaagtacgg gtcttttctt tttcatcgtc ctgaaggtac
    88981 aaaagagata gattctctta gtttaacacg gacgcatcag aaccttcccg gggaaaacag
    89041 tgcttgttta caattattag atatacacta aatcaattgc atcgcattgt gctaacgacg
    89101 cggcaaaacc ccggaagcgt acatcagcac gcgaccggag cgagtgagga agcctgcaaa
    89161 aaggcaggct tcaaagatgc cgttgagttt taaaccggcg gcagatcgaa cagtaacact
    89221 tcgctgtcgc tatccgcatg aatggagatt gcctgctcat cccagattgc cagaccatcg
    89281 ctggtcgagg ctttcacgcc gttaatggtg acattgcctt tcaccacctg aatccagacg
    89341 cggcgttcag cggcaatctg atgcaccgac tgctcatctt ttaccagcgc ccagcggtac
    89401 agttccatat cctgatacac tttcagcgaa ccatcacgcg catccggcga gagcaccagc
    89461 tgtttgccct gtacggcatc gaagcgacgc tgttcataac gcggcgtaat accgttttct
    89521 tcaggcatga tccagatttg atacagatgc agacgctcgg tgctgcttgg gttgtactct
    89581 gagtgacgaa tacccgtacc cgcactcata atctggaact cacccgccgg aacctgctct
    89641 ttattgccca tgctgtcctg atgctcaaca gtaccttcca gcacgtaggt caaaatttcc
    89701 atatctttat gcgggtgagt gccgaagccc tgccctgctt caatcacgtc gtcgttaatc
    89761 acgcgcagcg cggagaagcc cataaagttc ggatcgtaat agttggcaaa agagaaagta
    89821 tgccaggagt ccagccagcc atgatttgca tgaccacgtt catttgcttt gcgtaagtag
    89881 atcattttta ttcgccccct gaatgatttc gatgggctta gtgtggaccc gatccgcctg
    89941 ggatgataga gggtgaaaat tgacccctct gttcaaaaaa attgaactat tcgaggggcc
    90001 atcaaactta cttcgcgaga gtcactgtgg agggagaggc ttgctcaaat cctcgttcaa
    90061 ggatttccag attggtaaga acttcagatt ccttgacgta atttggcgca ccgttggtga
    90121 tggtttgata caacgcatca taaacgcgcc cgtaatcgcc catctccggc ttcatctctt
    90181 ctctgaccgt cacaccctcg tcattgacat actccagcac accgaccgaa tcatccgctg
    90241 cgaatcccgg ttcgcccggc ataatattag ccttgaggct ggtttcctgc tggtcgatac
    90301 cgtatttaat aaacgaacct ttcttaccgt gaacgataaa tttcggataa tcgattttca
    90361 ccagatggct ggttttgacg atggctttca ggtcgccata aaacagctgc gcttcaaagg
    90421 tgtcgtcagg attggcttta ttacgcaggc tgcggatgtc ataagcgacg tgatccgggc
    90481 gaccgaacag agaaataatc tggtccatcg tatgcacacc aagaccatag aacgcgccat
    90541 cctgcggcag cccaggtttg gtttctgcca ccgggcggta atagtcaaaa tggctttcca
    90601 cttcgacaat ctctcccaac ttgccacttt caatcgcttt tttcgctgtc aggaagcagg
    90661 aatcaaaacg acgattctga tacggcgtga cggtcagtcc tttgcttttc gccagcgcaa
    90721 acagctcctt cgcctgggca agcgtcgggg tgaacggttt ttcgaccagc acatttttcc
    90781 cggcttccag cgcgcgcttc gcgtactcga agtggctgtc tgcatgggtg cagaccacaa
    90841 ccagcttaac atcgggatcg tttagtactt cgtcgagatc gctggtgaaa tggatatgag
    90901 agtaaatggg ggcctgttct tccggcttcg catggcggcg aaaaatatgc gcgacatgcc
    90961 agctatcctt gcggttaagt acatacggca gatggtaacg ggtggtgctt ttgccgaagc
    91021 caataaaggc gcagttgatg accatgattt cgtccttttt aaggtgatta tttacacctt
    91081 agcgcaaagc ggacgtggtt cctactggag tgcgcataac gaacacaagc acttcccgtg
    91141 gataaattga aaacgaaaga tcaaaaaaaa agccagcacc cggctggcta agtaatactg
    91201 gaagcaatgt gagcaatgtc gtgctttcag gttctccgcg agggtcttcc tgatcgcaag
    91261 acaataataa tcattctcat tcgcacttgt ccaacacttt ttgcaaaaaa aatgcatttg
    91321 actcgcattt gaaagtcaat gatgttgaaa gggacattta ccccaaagag gacaaaggaa
    91381 tgagtgagat agtgatacgc catgctgaaa cgcgcgatta cgaggccatc aggcagattc
    91441 acgcccagcc ggaggtgtat tgcaacacgc tacaggtgcc tcatccttcc gatcatatgt
    91501 ggcaggagcg actcgccgat cgtcccggca tcaagcaact cgtcgcctgt attgatggag
    91561 acgtcgtggg ccatctcacc attgacgtgc aacagcgccc acgccgcagt catgttgcag
    91621 attttggtat ttgtgtcgac tcccgttgga agaatcgcgg cgtcgccagc gccctgatgc
    91681 gagagatgat tgaaatgtgc gacaactggt tgcgggtaga tcgtattgag ctaacggtgt
    91741 tcatcgataa cgcgccagca attaaagtct ataaaaaatt tggatttgag attgaaggga
    91801 ctggcaagaa gtacgcatta cgtaatggtg aatatgtcga tgcgtattat atggcgcggg
    91861 tgaagtaaga tagtgccctt tttctgagat ggaaaaaggg tgtcattcaa aatcggcata
    91921 ccttccttta atgtatttat tttcccaata aatatataag atttacctca taattacgtc
    91981 ctaaagtgta atattttatt ttttaatata tacgcctaca atttcctgga gtaaataaat
    92041 aacaattaac aagcataata gtgccattga taaaatagca tgccataaaa ggacttttca
    92101 tggatgagta atattgttta cctgacagta acg
//
LOCUS       DAOTRK010000004        83082 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.004, whole genome shotgun sequence.
ACCESSION   DAOTRK010000004 DAOTRK010000000
VERSION     DAOTRK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.004"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(20..277)
                     /gene="rpmA"
                     /locus_tag="QIE41_000274"
     CDS             complement(20..277)
                     /gene="rpmA"
                     /locus_tag="QIE41_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDS3529207.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(298..609)
                     /gene="rplU"
                     /locus_tag="QIE41_000275"
     CDS             complement(298..609)
                     /gene="rplU"
                     /locus_tag="QIE41_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDS3529208.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            868..1839
                     /gene="ispB"
                     /locus_tag="QIE41_000276"
     CDS             868..1839
                     /gene="ispB"
                     /locus_tag="QIE41_000276"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDS3529209.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            2067..2345
                     /gene="sfsB"
                     /locus_tag="QIE41_000277"
     CDS             2067..2345
                     /gene="sfsB"
                     /locus_tag="QIE41_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDS3529210.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(2393..3652)
                     /gene="murA"
                     /locus_tag="QIE41_000278"
     CDS             complement(2393..3652)
                     /gene="murA"
                     /locus_tag="QIE41_000278"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDS3529211.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(3707..3961)
                     /gene="ibaG"
                     /locus_tag="QIE41_000279"
     CDS             complement(3707..3961)
                     /gene="ibaG"
                     /locus_tag="QIE41_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDS3529212.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(4121..4414)
                     /gene="mlaB"
                     /locus_tag="QIE41_000280"
     CDS             complement(4121..4414)
                     /gene="mlaB"
                     /locus_tag="QIE41_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDS3529213.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(4414..5049)
                     /gene="mlaC"
                     /locus_tag="QIE41_000281"
     CDS             complement(4414..5049)
                     /gene="mlaC"
                     /locus_tag="QIE41_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDS3529214.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(5068..5619)
                     /gene="mlaD"
                     /locus_tag="QIE41_000282"
     CDS             complement(5068..5619)
                     /gene="mlaD"
                     /locus_tag="QIE41_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDS3529215.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(5624..6406)
                     /gene="mlaE"
                     /locus_tag="QIE41_000283"
     CDS             complement(5624..6406)
                     /gene="mlaE"
                     /locus_tag="QIE41_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDS3529216.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(6414..7223)
                     /gene="mlaF"
                     /locus_tag="QIE41_000284"
     CDS             complement(6414..7223)
                     /gene="mlaF"
                     /locus_tag="QIE41_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDS3529217.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            7433..8410
                     /locus_tag="QIE41_000285"
     CDS             7433..8410
                     /locus_tag="QIE41_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDS3529218.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPPDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            8424..9410
                     /gene="kdsD"
                     /locus_tag="QIE41_000286"
     CDS             8424..9410
                     /gene="kdsD"
                     /locus_tag="QIE41_000286"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDS3529219.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            9431..9997
                     /gene="kdsC"
                     /locus_tag="QIE41_000287"
     CDS             9431..9997
                     /gene="kdsC"
                     /locus_tag="QIE41_000287"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDS3529220.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLLPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            9994..10569
                     /gene="lptC"
                     /locus_tag="QIE41_000288"
     CDS             9994..10569
                     /gene="lptC"
                     /locus_tag="QIE41_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDS3529221.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            10538..11095
                     /gene="lptA"
                     /locus_tag="QIE41_000289"
     CDS             10538..11095
                     /gene="lptA"
                     /locus_tag="QIE41_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDS3529222.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            11102..11827
                     /gene="lptB"
                     /locus_tag="QIE41_000290"
     CDS             11102..11827
                     /gene="lptB"
                     /locus_tag="QIE41_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDS3529223.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            11875..13308
                     /gene="rpoN"
                     /locus_tag="QIE41_000291"
     CDS             11875..13308
                     /gene="rpoN"
                     /locus_tag="QIE41_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDS3529224.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            13331..13618
                     /gene="hpf"
                     /locus_tag="QIE41_000292"
     CDS             13331..13618
                     /gene="hpf"
                     /locus_tag="QIE41_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDS3529225.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            13736..14227
                     /gene="ptsN"
                     /locus_tag="QIE41_000293"
     CDS             13736..14227
                     /gene="ptsN"
                     /locus_tag="QIE41_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDS3529226.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            14273..15127
                     /gene="rapZ"
                     /locus_tag="QIE41_000294"
     CDS             14273..15127
                     /gene="rapZ"
                     /locus_tag="QIE41_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDS3529227.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            15124..15396
                     /gene="npr"
                     /locus_tag="QIE41_000295"
     CDS             15124..15396
                     /gene="npr"
                     /locus_tag="QIE41_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDS3529228.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            15609..16241
                     /gene="yrbL"
                     /locus_tag="QIE41_000296"
     CDS             15609..16241
                     /gene="yrbL"
                     /locus_tag="QIE41_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDS3529229.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAAQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALTIALQKDLQPRESKTLTLTSREA"
     gene            complement(16238..16966)
                     /gene="mtgA"
                     /locus_tag="QIE41_000297"
     CDS             complement(16238..16966)
                     /gene="mtgA"
                     /locus_tag="QIE41_000297"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDS3529230.1"
                     /translation="MSKSRLTVFSFIRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVNMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(16963..17616)
                     /gene="elbB"
                     /locus_tag="QIE41_000298"
     CDS             complement(16963..17616)
                     /gene="elbB"
                     /locus_tag="QIE41_000298"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDS3529231.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(17846..20182)
                     /gene="arcB"
                     /locus_tag="QIE41_000299"
     CDS             complement(17846..20182)
                     /gene="arcB"
                     /locus_tag="QIE41_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDS3529232.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(20278..21207)
                     /locus_tag="QIE41_000300"
     CDS             complement(20278..21207)
                     /locus_tag="QIE41_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDS3529233.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            21882..26342
                     /gene="gltB"
                     /locus_tag="QIE41_000301"
     CDS             21882..26342
                     /gene="gltB"
                     /locus_tag="QIE41_000301"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDS3529234.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            26355..27773
                     /gene="gltD"
                     /locus_tag="QIE41_000302"
     CDS             26355..27773
                     /gene="gltD"
                     /locus_tag="QIE41_000302"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDS3529235.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            27957..29069
                     /locus_tag="QIE41_000303"
     CDS             27957..29069
                     /locus_tag="QIE41_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDS3529236.1"
                     /translation="MESLSEGTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSTRNLWQFKNFYICFQRIEI
                     VQTLSAQFTPTILQTVSGESCHLAQLAKSFPLPWSTYVRLLSVKKADARSFYEKETLR
                     CGWSARQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLEFLELK
                     DEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLL
                     IVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAYYALAGLP
                     NTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(29129..29593)
                     /locus_tag="QIE41_000304"
     CDS             complement(29129..29593)
                     /locus_tag="QIE41_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDS3529237.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPDEIKKVVVKVKADLMA"
     gene            complement(29590..30465)
                     /gene="nanK"
                     /locus_tag="QIE41_000305"
     CDS             complement(29590..30465)
                     /gene="nanK"
                     /locus_tag="QIE41_000305"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDS3529238.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTHAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(30462..31151)
                     /locus_tag="QIE41_000306"
     CDS             complement(30462..31151)
                     /locus_tag="QIE41_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDS3529239.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(31199..32689)
                     /gene="nanT"
                     /locus_tag="QIE41_000307"
     CDS             complement(31199..32689)
                     /gene="nanT"
                     /locus_tag="QIE41_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDS3529240.1"
                     /translation="MSTSTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(32798..33691)
                     /gene="nanA"
                     /locus_tag="QIE41_000308"
     CDS             complement(32798..33691)
                     /gene="nanA"
                     /locus_tag="QIE41_000308"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDS3529241.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(33813..34604)
                     /gene="nanR"
                     /locus_tag="QIE41_000309"
     CDS             complement(33813..34604)
                     /gene="nanR"
                     /locus_tag="QIE41_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDS3529242.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            34984..36351
                     /gene="dcuC"
                     /locus_tag="QIE41_000310"
     CDS             34984..36351
                     /gene="dcuC"
                     /locus_tag="QIE41_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDS3529243.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(36395..36892)
                     /gene="sspB"
                     /locus_tag="QIE41_000311"
     CDS             complement(36395..36892)
                     /gene="sspB"
                     /locus_tag="QIE41_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDS3529244.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36898..37536)
                     /gene="sspA"
                     /locus_tag="QIE41_000312"
     CDS             complement(36898..37536)
                     /gene="sspA"
                     /locus_tag="QIE41_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDS3529245.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(37931..38323)
                     /gene="rpsI"
                     /locus_tag="QIE41_000313"
     CDS             complement(37931..38323)
                     /gene="rpsI"
                     /locus_tag="QIE41_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDS3529246.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(38339..38767)
                     /gene="rplM"
                     /locus_tag="QIE41_000314"
     CDS             complement(38339..38767)
                     /gene="rplM"
                     /locus_tag="QIE41_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDS3529247.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(38986..40113)
                     /gene="zapE"
                     /locus_tag="QIE41_000315"
     CDS             complement(38986..40113)
                     /gene="zapE"
                     /locus_tag="QIE41_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDS3529248.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQYCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPLMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            40307..40705
                     /locus_tag="QIE41_000316"
     CDS             40307..40705
                     /locus_tag="QIE41_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDS3529249.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            40859..42226
                     /gene="degQ"
                     /locus_tag="QIE41_000317"
     CDS             40859..42226
                     /gene="degQ"
                     /locus_tag="QIE41_000317"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDS3529250.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            42316..43383
                     /gene="degS"
                     /locus_tag="QIE41_000318"
     CDS             42316..43383
                     /gene="degS"
                     /locus_tag="QIE41_000318"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDS3529251.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(43446..44384)
                     /gene="mdh"
                     /locus_tag="QIE41_000319"
     CDS             complement(43446..44384)
                     /gene="mdh"
                     /locus_tag="QIE41_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDS3529252.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEKNALEGMLDTLKKDIALGEEFVNK"
     gene            44819..45289
                     /gene="argR"
                     /locus_tag="QIE41_000320"
     CDS             44819..45289
                     /gene="argR"
                     /locus_tag="QIE41_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDS3529253.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            45654..45917
                     /gene="yhcN"
                     /locus_tag="QIE41_000321"
     CDS             45654..45917
                     /gene="yhcN"
                     /locus_tag="QIE41_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDS3529254.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(45973..46245)
                     /locus_tag="QIE41_000322"
     CDS             complement(45973..46245)
                     /locus_tag="QIE41_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDS3529255.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(46337..48304)
                     /gene="aaeB"
                     /locus_tag="QIE41_000323"
     CDS             complement(46337..48304)
                     /gene="aaeB"
                     /locus_tag="QIE41_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDS3529256.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(48310..49242)
                     /gene="aaeA"
                     /locus_tag="QIE41_000324"
     CDS             complement(48310..49242)
                     /gene="aaeA"
                     /locus_tag="QIE41_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDS3529257.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(49250..49453)
                     /locus_tag="QIE41_000325"
     CDS             complement(49250..49453)
                     /locus_tag="QIE41_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDS3529258.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            49636..50565
                     /gene="aaeR"
                     /locus_tag="QIE41_000326"
     CDS             49636..50565
                     /gene="aaeR"
                     /locus_tag="QIE41_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDS3529259.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(50692..52137)
                     /gene="tldD"
                     /locus_tag="QIE41_000327"
     CDS             complement(50692..52137)
                     /gene="tldD"
                     /locus_tag="QIE41_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDS3529260.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(52293..56093)
                     /gene="yhdP"
                     /locus_tag="QIE41_000328"
     CDS             complement(52293..56093)
                     /gene="yhdP"
                     /locus_tag="QIE41_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDS3529261.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWNQWKLLPGTEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGVADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKAGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(56161..57630)
                     /gene="rng"
                     /locus_tag="QIE41_000329"
     CDS             complement(56161..57630)
                     /gene="rng"
                     /locus_tag="QIE41_000329"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDS3529262.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(57620..58213)
                     /gene="yhdE"
                     /locus_tag="QIE41_000330"
     CDS             complement(57620..58213)
                     /gene="yhdE"
                     /locus_tag="QIE41_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDS3529263.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAVQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(58222..58710)
                     /gene="mreD"
                     /locus_tag="QIE41_000331"
     CDS             complement(58222..58710)
                     /gene="mreD"
                     /locus_tag="QIE41_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDS3529264.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(58710..59813)
                     /gene="mreC"
                     /locus_tag="QIE41_000332"
     CDS             complement(58710..59813)
                     /gene="mreC"
                     /locus_tag="QIE41_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDS3529265.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(59879..60922)
                     /gene="mreB"
                     /locus_tag="QIE41_000333"
     CDS             complement(59879..60922)
                     /gene="mreB"
                     /locus_tag="QIE41_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDS3529266.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(61227..63167)
                     /gene="csrD"
                     /locus_tag="QIE41_000334"
     CDS             complement(61227..63167)
                     /gene="csrD"
                     /locus_tag="QIE41_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDS3529267.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            63319..64293
                     /locus_tag="QIE41_000335"
     CDS             63319..64293
                     /locus_tag="QIE41_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDS3529268.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESCLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAGAIINNQIQGRTLVKVN"
     gene            65270..65740
                     /gene="accB"
                     /locus_tag="QIE41_000336"
                     /gene_synonym="fabE"
     CDS             65270..65740
                     /gene="accB"
                     /locus_tag="QIE41_000336"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDS3529269.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            65751..67100
                     /gene="accC"
                     /locus_tag="QIE41_000337"
     CDS             65751..67100
                     /gene="accC"
                     /locus_tag="QIE41_000337"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDS3529270.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            67209..67451
                     /locus_tag="QIE41_000338"
     CDS             67209..67451
                     /locus_tag="QIE41_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDS3529271.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            67441..68892
                     /gene="panF"
                     /locus_tag="QIE41_000339"
     CDS             67441..68892
                     /gene="panF"
                     /locus_tag="QIE41_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDS3529272.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            68904..69785
                     /gene="prmA"
                     /locus_tag="QIE41_000340"
     CDS             68904..69785
                     /gene="prmA"
                     /locus_tag="QIE41_000340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDS3529273.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            70114..71079
                     /gene="dusB"
                     /locus_tag="QIE41_000341"
     CDS             70114..71079
                     /gene="dusB"
                     /locus_tag="QIE41_000341"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDS3529274.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            71105..71401
                     /gene="fis"
                     /locus_tag="QIE41_000342"
     CDS             71105..71401
                     /gene="fis"
                     /locus_tag="QIE41_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDS3529275.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            71487..72371
                     /gene="yhdJ"
                     /locus_tag="QIE41_000343"
     CDS             71487..72371
                     /gene="yhdJ"
                     /locus_tag="QIE41_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDS3529276.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            72455..72634
                     /locus_tag="QIE41_000344"
     CDS             72455..72634
                     /locus_tag="QIE41_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDS3529277.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(72637..73299)
                     /gene="acrS"
                     /locus_tag="QIE41_000345"
     CDS             complement(72637..73299)
                     /gene="acrS"
                     /locus_tag="QIE41_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDS3529278.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLWELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQNLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VI"
     gene            73698..74855
                     /gene="acrE"
                     /locus_tag="QIE41_000346"
     CDS             73698..74855
                     /gene="acrE"
                     /locus_tag="QIE41_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDS3529279.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLSRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESAHINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQSDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADSASK"
     gene            74867..77971
                     /gene="acrF"
                     /locus_tag="QIE41_000347"
     CDS             74867..77971
                     /gene="acrF"
                     /locus_tag="QIE41_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDS3529280.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGVLAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSASSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            78224..78445
                     /locus_tag="QIE41_000348"
     CDS             78224..78445
                     /locus_tag="QIE41_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529281.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            78876..79901
                     /locus_tag="QIE41_000349"
     CDS             78876..79901
                     /locus_tag="QIE41_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS3529282.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKEANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            79969..81150
                     /locus_tag="QIE41_000350"
     CDS             79969..81150
                     /locus_tag="QIE41_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDS3529283.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFIVTVVIAIAISVGLFRFNKTYQIKTGQLRRTWP
                     VAVMLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            81160..82263
                     /locus_tag="QIE41_000351"
     CDS             81160..82263
                     /locus_tag="QIE41_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDS3529284.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            82271..83029
                     /locus_tag="QIE41_000352"
     CDS             82271..83029
                     /locus_tag="QIE41_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDS3529285.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLALHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT        20330 a        21766 c        20948 g        20038 t
ORIGIN      
        1 ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg tttttcgggc
       61 ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc
      121 aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg atgctacccg
      181 ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac
      241 cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc tcaggtctta
      301 ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatggccct gctgcttacg
      361 atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac cgtgagcaac
      421 aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga cttcttcacc
      481 gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga tgtccagctt
      541 ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac tttggaaaac
      601 cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga gtgcgctata
      661 aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa gtgctacagt
      721 caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc cattttttca
      781 gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca cagtaaggta
      841 atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg
      901 caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga cgtccaactg
      961 atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg tccgatgatt
     1021 gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac cattgctgcc
     1081 ctgatcgagt ttatccacac ggcgactctg ctgcacgacg acgttgtgga tgaatcagat
     1141 atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag cgtgctggta
     1201 ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggctc gctcaaagtg
     1261 ctggaagtca tgtcagaagc cgtaaacgtc atcgcagaag gtgaagttct gcaactgatg
     1321 aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta tagcaaaacc
     1381 gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg tacgccggag
     1441 gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca gttgatcgac
     1501 gatttactcg attacaatgc cgatggcgaa cagttaggta aaaacgtcgg cgacgatctg
     1561 aacgaaggta aaccgacgct gccgctgctg catgcaatgc atcatggcac accagaacag
     1621 gcacagatga ttcgtaccgc catcgaacag ggtaacggtc gccatcttct ggaaccggtt
     1681 ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc cgaggaagaa
     1741 gcagacaaag ccatcgcagc gttacaggtg ctcccggaca ccccgtggcg agaagcactc
     1801 atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccccg catcccgcgc
     1861 agcgcgcggg atggttctag taaattccat aaaaacactt attcagctct ataacacctg
     1921 aaaaataggg ctgtacatct ttatatattc tgaatattca cactctttac aggaactttt
     1981 tagaacaata cgccatcagg agtatagtga tgcccgacag aagaaatgtt ctgaatgaaa
     2041 gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg catcccgctg
     2101 acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggaatct cgcagaaatg
     2161 gtttgagttc ctcaacgctg gcgaacgcat tatcgcgccc ctggccgaaa ggagagatga
     2221 ttattgcgaa agccctggga actgacccct ggattatctg gccatctcgc taccatgatc
     2281 cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact aaaccgaaaa
     2341 aataagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac aattactcgc
     2401 ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg cgttcgtagc
     2461 cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata cagccagcca
     2521 gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca gaaagttttt
     2581 caacaccgtg acaaataacg gtattgcttt cgatttcggc gtgcgcgccc atacggctca
     2641 gctctggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaag ccggtccctt
     2701 ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat gccggatgcg
     2761 gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg ctaatccagt
     2821 cttcgccgac ttcgatgtct gctccagcgt cacgcagttt cgccagcacg gcgtccagag
     2881 tatctggctg cgcgttacgg cagataattt tgccgcgaga gatcgccgcc gccaccagga
     2941 aagtaccggt ttcgatacga tccggcagca cgcgatagac accgccgcct aaacgttcca
     3001 caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc agcgtaatca
     3061 ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata atcgtggtgc
     3121 cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg actttatcca
     3181 tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa ccttcttcca
     3241 gtttgatggt cgcgcctaat tgttcgagac cagaaatgtg tagatcaacc ggacgtgcac
     3301 caatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct accagcggcc
     3361 ccagcgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg cagaatacat
     3421 taacatcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca cccagctggc
     3481 ttagcagctt cattgatgta tcgacgtctt tcagtttcgg gacgttctgg atctctaccg
     3541 gttcttccgc cagtagtgcg gcaaaaagga taggcagagc agcattttta gcgccggaaa
     3601 ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc atttagtttg
     3661 ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa aagccgttca
     3721 gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca gcatgaatgc
     3781 ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt ttaacccgac
     3841 tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca tcgccggaaa
     3901 cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca ttattttcca
     3961 tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa gttgcgctgg
     4021 cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc aggcaggttt
     4081 gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc agaacatcag
     4141 caggcaaatt atacaatttc gccagggtat acactttgtc gtttaccccc tgaagcgtca
     4201 cgttgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc agtccacccg
     4261 tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc gcttcctcac
     4321 gcatttccca aagcggtagc agaacgtcct gatccagctc tccggataac gccagcgtgt
     4381 cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca gagtgatttt
     4441 ctgttgagaa atcgatttca gttgtgcagt caggccgtcg atacctttgg tacgcagcag
     4501 cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa tcatgtcgta
     4561 agcctgccag ttgcccgtct gggagttttt acgccactgg aagtccagac gcaccggcgg
     4621 gcggccattc gggtcaataa tggtaacgcg aataggcaca atggttttat cgcccagcgg
     4681 ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct gaccgtaagc
     4741 ctgcttcagg tactcacgga aagcggcaaa gtaagcatca cgttgagcag gggtcgcact
     4801 cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt atggcaacag
     4861 ttcctgatcg acaatggtac gcagataatc cgggttggcc cgaatttgcg gttgctcatt
     4921 cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttatacg gattggtctg
     4981 gtctgccgcg gttgccgcac tcagcggtgc aatcaccagc aaagcgacca tcattaaacg
     5041 tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt cagtggtttc
     5101 attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat cgcctttact
     5161 accgtaaagg aactgaccaa taagatcttc cagcaccatc gcagacttgg tgtcctgaat
     5221 tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac cgacgtttaa
     5281 tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac tggtatcagg
     5341 aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg ttttcgggtc
     5401 cagcgtaata tccgccaccc ggcccacaac aacaccacca atgctgaccg gagagcgcgc
     5461 tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt cagtacgtat
     5521 ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagtagtg ctgctaataa
     5581 aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc ccaaacatca
     5641 atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca acggtgcgag
     5701 tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta aacaacgaaa
     5761 tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc agatccatac
     5821 gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg cctttccaac
     5881 tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc gtcaacagtg
     5941 gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc ggatccaccg
     6001 ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt tctgcggtta
     6061 gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc agttcacgca
     6121 gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg gtcagaacca
     6181 gataaccttg cagccccaac accattccga tgaacacgcc agaaactaca ataatcagca
     6241 tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt ttgcgaaatt
     6301 ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac gttctcaggg
     6361 ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt ggcttaactc
     6421 cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac aggcccgtca
     6481 gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa cgcctgggca
     6541 ctaccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatcggc aatacttaac
     6601 acttccggca catcgtgaga aaccaccaca caagtcacac ccaacgcgct gttcagctca
     6661 gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa gggttcatca
     6721 aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg ccgcgccatc
     6781 ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac ggcctccagc
     6841 ttcatcatca ccgtactatg caacaatggc gcgggaagtt gggtatgttc gcgcagtgga
     6901 taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg aaataacatg
     6961 ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat attctcacca
     7021 tcgaaaagaa tctcaccatg atctggtgcg atttgcccgc caatcagacg gagtagcgtc
     7081 gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg cacggtcagg
     7141 gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg catatcgact
     7201 aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt tctaagcatg
     7261 gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg tggttagcga
     7321 aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat ccgttacggc
     7381 tttctgaaaa tcttcagcgg accggcgagt atacctgaag aaaggacgtt agatgctttt
     7441 agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg accgcctggt
     7501 ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca tcggcatgac
     7561 ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg cgtctttgca
     7621 cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca atatattgct
     7681 gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattctgatg ttctacgccg
     7741 tgaattaccc ttaatgttgt tggtcagcgt ggtggccggt tccgtactct atgacggaca
     7801 acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc tgctgttcat
     7861 tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca gagagcagct
     7921 tgcagagttg ccgcctgacg gtggattgcc cgtcgcgttt ttatggctcg gcattgcgct
     7981 tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc tggcgaatta
     8041 ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa ccagcctgcc
     8101 ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg ctgtcggaaa
     8161 tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg cgctgataac
     8221 gccaggagag attgatccac tggcgtacag tcgtgactac agcgtgatgt tgctggtgag
     8281 cattattttt gcgttgctat gctggcggcg ctccccgcaa ccgggccgtg gtgtaggggt
     8341 attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat cgccaatact
     8401 cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt tttgactttc
     8461 agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag cttgatcaat
     8521 acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa gggaaagttg
     8581 tcgtcatggg gatggggaaa tcggggcata ttgggcgcaa aatggcggca acgtttgcca
     8641 gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt gatttaggca
     8701 tggtcacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc agcgaaatca
     8761 cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc accggtcgcc
     8821 cggagagcag catggcgcgc gccgcagatg ttcatctgtg tgttaaagta gcgaaagaag
     8881 cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt atgggcgatg
     8941 ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc cgaagatttt gcgctctcac
     9001 atccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt atgcatacgg
     9061 gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcattg ctggaagtta
     9121 cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt gaaggcatct
     9181 ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt cagttaagta
     9241 ttgccgatgt tatgacgccg gggggaatac gcgtgcgccc tggcattctg gccgttgagg
     9301 cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat ggcgaccatt
     9361 tactcggtgt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa agattcaagg
     9421 ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc ctgtcagcgc
     9481 cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg atggcgtact
     9541 gtcagatggc ctgatttata tgggcaataa tggcgaagag ctgaaagcgt tcaatgttcg
     9601 tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta tcattaccgg
     9661 gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc acttgtatca
     9721 ggggcagtca aacaaactga tcgcctttag cgatttgctg gaaaaactgg cgattgcccc
     9781 ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg aaaaagtggg
     9841 tttaagcgtc gccgtggccg atgcgcatcc actgttgctc ccgcgcgccg attacgtgac
     9901 gcgcattgct ggcggtcgtg gtgcagtgcg tgaagtttgc gacttattac tcctggcgca
     9961 gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg ttgggttatc
    10021 attgtgctat cactggcggt tctggtgatg atcggcatta atatggccga aaaagacgat
    10081 accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca tacggacacg
    10141 ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca cgttgaatat
    10201 tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt tgataaggat
    10261 aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga ccggatgctc
    10321 tatttatatg gacacgttga agtcaacgca cttgtgccgg actctcaact tcgcagaatc
    10381 acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga agacctcgtc
    10441 acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa cttacgcagc
    10501 aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa caaacaaact
    10561 cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg catttgccgt
    10621 aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc ttgatatgca
    10681 aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca tcaaaattaa
    10741 tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag tgattgacgg
    10801 ctacggtaaa ccggcaacgt tctaccagat gcaggacaat ggtaaacccg ttgaaggtca
    10861 cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg gtaatgctta
    10921 tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg tgaaagagca
    10981 gaaaatgcag gctttcagcg acaaaggtaa gcgcgtaacc accgttctgg tgccgtcgca
    11041 gctgcaggac aaaaacaaca aaggccagac cccggcacag aagaagggta attaattcgt
    11101 tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc gcgtggtaga
    11161 agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc caaacggtgc
    11221 cggtaagacc accactttct acatggttgt aggcattgtg ccgcgcgatg cgggcaacat
    11281 cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc gccgcggtat
    11341 cggctatctg ccgcaggaag cctccatttt ccgtcgcctc agcgtttacg ataacctgat
    11401 ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc gcgcgaacga
    11461 gctgatggaa gagtttcaca ttgaacactt gcgtgacagc atggggcagt cactctccgg
    11521 gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga aatttattct
    11581 gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta aacgcatcat
    11641 tgagcacctg cgcgacagcg gcctgggcgt actgatcact gaccacaacg tgcgtgaaac
    11701 actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg cccacggcac
    11761 gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg aagacttcag
    11821 actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct gaacatgaag
    11881 caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct ccaacaggca
    11941 attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca ggcgctggag
    12001 agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg cgaaacgcaa
    12061 gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc ggaagagctg
    12121 ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg caccagcggt
    12181 gactacattg acgacgagct gccggtctat cagggcgaaa cgacgcagac cttgcaggat
    12241 tacctgatgt ggcaggtcga gttgacaccg ttttctgaca ctgaccgcgc tattgctacc
    12301 tctatcgtcg atgccgttga tgacaccggt tatctgactg tcccactgga agatattctc
    12361 gaaagtatgg gcgatgaaga gattgacatc gacgaagttg aagccgtcct taagcggatc
    12421 caacggtttg atccggtcgg tgtggcagca aaagatctgc gtgactgtct gctgatccaa
    12481 ctctcccaat tcgataaaac cacgccatgg ctggaagagg ccagactgat cattagcgat
    12541 catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac gcgtctgaaa
    12601 gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg ccccgggcag
    12661 tcgatccaga cgggcgaacc tgagtatgtc attccagatg tgctggtgcg taagcataac
    12721 ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat caaccagcac
    12781 tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat ccgcagcaat
    12841 ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac gctactgcgc
    12901 gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggcga agaatatatg
    12961 aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc gacgatatct
    13021 cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact gaagtatttc
    13081 ttttccagtc acgtcaatac cgagggcggc ggcgaagctt cctccacggc gattcgtgcg
    13141 ctggttaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga cagcaagtta
    13201 acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc gaagtaccga
    13261 gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc caaccgataa
    13321 ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg aggcactgcg
    13381 cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa tcaaccaggt
    13441 ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac tgcatgtaaa
    13501 cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca ttgatggctt
    13561 aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac aacactaatt
    13621 gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc catttgtacg
    13681 gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg aaattatgac
    13741 aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta cgcgaagccg
    13801 cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg cggcgaaaca
    13861 acttagtctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa aaatgggcag
    13921 taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag atactctgcg
    13981 cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca tcgacaacca
    14041 accggtggac ttactttttg ccctgctggt gccggcagac caaactaaaa cgcacctgca
    14101 tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc gtttacgcgc
    14161 agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta ctccggatga
    14221 agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt acatggtact
    14281 gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg cgctggaaga
    14341 tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc tggctcgaac
    14401 cctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata tgccggagtc
    14461 accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac cgcaactact
    14521 gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc gccgactgca
    14581 tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa gcgatttgct
    14641 ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt ccgttcacga
    14701 gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac tgaccatggt
    14761 ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg tctttgacgt
    14821 gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag gtcttgataa
    14881 acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct accagacgcg
    14941 aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct acttgacggt
    15001 cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc aactggcaga
    15061 ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg aaaaacgtaa
    15121 accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca tgcccggcct
    15181 gcaatgaagc tgtttgaatt aatgcaaggt tttgacgcag aagtgctgtt acgtaatgac
    15241 gaaggtaccg aggccgaagc caacagcgtt attgcactgc tgatgctgga ttctgccaaa
    15301 ggacggcaga ttgaagttga agcgaccggt ccacaggaag aggaagcact ggccgccgtt
    15361 atcgccctct ttaattctgg ttttgatgaa gattaatctt catcactttg acattcaaat
    15421 cccttcaagc tcccccgaca ataagaaaag cgcgtactga aatcgttctc aatcaacgtc
    15481 atttgtacat ttcgtgcgct tttcactttc agaagaacat taagaaaacc ttaagaagca
    15541 ttgtttaggt ttcgtttaag ttaatcgacc atactggaga tcgtcagaaa atatttccag
    15601 gagatggcat gattcgctta tctgaacaaa gtcccctggg caccggaaga catcggaaat
    15661 gttatgcgca tccggaagat gcccaacgct gtatcaagat tgtctaccat cgtggcgatg
    15721 gtggcgataa agagatccgc cgtgagttaa agtactacgc gcatcttggt cgccggttaa
    15781 aagactggag tggaataccg cgctatcacg gtaccgttga gacggattgc ggaaccggtt
    15841 atgtctacga tgtgattgca gattttgacg gcaaaccctc cattacgctg accgaatttg
    15901 ctgcacaatg ccgttacgaa gaagacattg cacaactgcg ccagctactg aaacagctga
    15961 agcgttattt gcaggataac cgtatcgtga cgatgtcgct gaagccgcag aatatcctct
    16021 gtcaccgcat cagtgagtca gaggttatcc ccgtcgtctg cgacaacatt ggtgaaagca
    16081 cattgattcc actggcgacc tggtcgaagt ggtgttgtct gcgtaagcaa gagcggttat
    16141 ggaaacgatt tatcgcccaa ccggcgctga ctattgccct gcagaaagat ctgcaacctc
    16201 gtgaaagcaa aacgctgacc ctcacttcgc gcgaagctta atccagctgg tgttgttgca
    16261 taaacgactc accgcctaac tgatacatct gccgtaaaat ccacgcctga cggctacgca
    16321 cgtagcccga tggcgcggag actttgaaac gaagtggatt aggtaataca gctgccagta
    16381 atgcagcttc cgaccgggta agcttgctcg cgggtttgtg gaaataacgt tgtgccgcag
    16441 cttcgacgcc aaacacaccg tcgccaaatt cggcgatatt caggtaaacc gtcaggatac
    16501 gctttttgct ccagaccgtt tctatcccca gcgttaatcc ggcttccagc ccttttcgaa
    16561 cccagctacg cccatcccat aaaaagagat ttttggccgt ctgctgagaa atcgttgatg
    16621 caccgcgaat acggttttca ttgcgttcgt tgtgcgccag ggcttgctca atggaagcga
    16681 catcaaagcc ccagtgctca ggaaacttct gatcttctgc ggcaatcacc gccagtccca
    16741 tccacggcga gatttgatcc atgttgaccc agtcagaatg tgccacgtaa cgaaaattgc
    16801 catgcagcca ggcgctgact tgccgctcga ccattaccgc agagaaagga acaggcgcaa
    16861 cgctaaacaa cgcaatgccc ccaccccaga aaacggcgag gacaaccatt aaccgcagaa
    16921 gaaaacggcg aatgaaacta aacaccgtta agcggctttt actcattccg ccaggaccag
    16981 cacgcgggaa accagcttat caatgccgct cgccgcttct gcaatgttct gcgccagcat
    17041 atatgccggg gtggtgacaa tcttattgtc ttcatcaacc acgatatcat cgacaggaca
    17101 cggcacatgc tccgcgccca tctcttccag cacttctgcg gtatcgatat cagtaccgat
    17161 ggtcaaacgc agcgggaaat cgaaaatttt cggcagcatc gccggggcaa tacacataaa
    17221 accaagcggt tttccggcct gatgcatcgc ttgtgccagc gcctttaatt cacggtcaac
    17281 ggtgcattcg ctaccaagac tggcaaaatt gcttaaattc ttcgccgcgc caaaaccccc
    17341 cggcacaatc aacgcatcca gttcagcggc atcggcctgg gccagaggac ggatttcacc
    17401 gcgcgttatt cgtgccgctt caatcagcac attgcgcgtt tccgtcatcg cttcgcccgt
    17461 taaatggttg ataacatcaa cctgctgctt atccggtgca aagcagaccg cctgcgcacc
    17521 gctgcgtgaa atagctaaca acgtcaacac cgcttcatga atttcagaac cgtcatagac
    17581 gccgcatccg ctcagaatta cgccaatttt cttcattgtg atcatccttt cgcaattgac
    17641 tgaaacacat taattttttt aataaaaatg ctacgcatca cacatttaac tgattcatgt
    17701 aacaaatcat ttaagttttg ctatcttaac tgcgtgcggc ctgaaaaaca gtgctgtgcc
    17761 cttgtaactc atcataataa tttacggcgc agccaagatt tccctggtgt tggcgcagta
    17821 ttcgcgcacc ccggtctagc cggggtcatt ttttagtggc ttttgccacc cacgctttca
    17881 gtacttctac gtcgtgacgc cactcttctt tcatctcttc aatccattca ccgacgttat
    17941 cttcccaggc gggaaggtca ggagactgaa tttgctgacc cagctgttgc agatggcgta
    18001 accccactga ccccgccgca cctttaattt tatgtccttc ctcaacaatg ccttttttat
    18061 cctgcgccgt cagattcgac tccagcacgc tgacatagcc cggcatcatc ttctcaaaca
    18121 ccgctaaccc gtcggtgatc agcttcggtc ctacaagttc gagatactgt tccagcatgg
    18181 gaatatcgag caatgcttct gatttactgt tctcttctgt cgtcaccgta ctctcctcat
    18241 catcctgggt atcccagaat ttcttgatca tcgcggttag cgccggaaca gaaagcggct
    18301 tactcagcac atcatccatt ccagcattga ggtactcttg cttgtctttc agcacgttag
    18361 cggttaaggc caccagcggc ggtaaatcct cgcgcggata acgcttcgtc agttcacgag
    18421 agatatccag cccggtcata tctggcaact gaatatccag caataccagg tcgtattcgc
    18481 ccggtttaaa catctccagc gccgccttgc cggtcatggc gacatcaacg ctgttaccta
    18541 atttttccag cacagaacgc gcgacaatca cgttcagttc aatgtcttcc accagcagca
    18601 cattcagcgc cggtaaaggc atatcgtctt catcaaacgc atcatcgacc tcttcagcca
    18661 ccgacggtgc gtggatcgtc aacgtaaagg ttgaaccttt accctgttcg ctggtaaccg
    18721 taatatcgcc gcccatattt ttcgccagac gacgagaaac ggccagacca ataccggtgc
    18781 cggtggcagg tttaccgcca tgactgtctt tcacctggta atacatggca aagattttat
    18841 ccagctcatc ctgcggaatg ccgataccag agtcttccac ttcaaaatgc agcatatcgc
    18901 cttcatcgta gcgcacgcgc acggtaacct ggccttgctg ggtgaatttg acggcgttac
    18961 tgatgagatt ccacaggatc tgccgtaaac gcgtcccgtc ggtaatgacc tgatgcggta
    19021 atggcagcgt aggctccaga ttaaagcgca atcctttttg ttgcgcctgc aatgcggaga
    19081 gattttccag atcggcaagg aagctggtga aatcaaccgg ctgattatca agctggacct
    19141 tacgccgttc catcttatcc atgtcgataa tatcgttgaa gatattcccg agcgtgacgg
    19201 ccgaaacatg gatggttttg agatattttt cctgctcggc ggtgagttcg gtatccagca
    19261 gaatgcggct caggccgacg ataccattca gcggtgtacg caattcgtga ctgatggtgg
    19321 agataaacgt cgttttgtcg cggctggccc gttcaagcgc atcctgatac cgcttacgct
    19381 cggtaatgtc gcgaccaaaa cccatcaaac cgtgacgttt acccacgcgg tcgtagtacg
    19441 gcactttacg gatttcaaaa caggctttgc gcccgtccgg gtaatccagc cactgttcat
    19501 aggtcagtga cacattatga cggaacactt tttcatcggt ttcaatgact tttgcggcgg
    19561 cttccggtga gtaaacatca gcaggtttca ggtgaaccag ttgtttttcg ctttttccgg
    19621 tcagcagctc catcgcgcgg ttacagccgg aaaactcttt atcttcgtta cgataaaaaa
    19681 ccaggtcggg tgaagcatca agaaaggaac gtaagaatga ggattgctgt tcgagctgaa
    19741 tttgtgtctc ttcgcgctct ttaatttcaa ttttcagttg gccgaaggtt tcctgcagtt
    19801 ctgcttccgc tttttcacga acggcgattt cctgatttag ctgggcaata ttatctttta
    19861 actgaacgtt gaggctcaaa tcgcgctcgc gcatctcctc cagtttttgc accagccgtg
    19921 acagacgttg ccgtgactcc tccagttgct cgacgaccac cgatagaaag tagaccgccc
    19981 acggcgtaat cagcaaacca aagaagatag aacgaataac atcaatgctt tcgacctgac
    20041 catgcagcac catggttacc gccatttgta ccacaatggc aagaacgacg agggccagcg
    20101 ccagcaacat tgagaagcgc accagaccta acttcatcat caggtcaaca taatactgcg
    20161 ccagcagacg aatttgcttc attagggaat tccttcacga caacctgccc caataatacc
    20221 caattctgac agttacgtta taaattgtgt gagaaatgcg gaattcccct caatggtcta
    20281 ctccgttgga ggtagccacg ggcgtcccag cgccgatccc tgtacgccat gttcattcag
    20341 atagcgatcc agctcgacca ttcccgtcca tcgattttcg caccacagcg gagcaagcaa
    20401 cgtcgggcga cgggcgctgg cggaaatgcg gtggtagatc acttccggtg gcgtatggcg
    20461 aatcatttct ccggcagtga gcgtgtaatc ctccagttca ataccattca aacgccccgc
    20521 ttcccaggct ttcgccataa tgctgccttt tactatatgc agcggatgca gctttatgcc
    20581 gtccacgccg gtttcaacca ctcgctccag cgtttgcaaa cattcagcct gcccttcgcc
    20641 aggcaggccg acaattaagt gagaacacac cttcagcccg cgctcacgcg ccagctgcgt
    20701 cgtgcgctga taacaggcaa aatcatgacc gcgattgatg cgatgtagcg ttttgtcgtg
    20761 cgcggtttgt agccccagct ccagccacac ttcgtagccc tggtccttat attcgcaaag
    20821 cagatccaac accgcatccg gcacgcagtc cgggcgggta ccaacacaca aaccgacaat
    20881 attggcctgg ctcaccgcct gctgatacat agaacgcagc acctgaactt ccgcaaaggt
    20941 gctggtatac gcctgaaagt aggccagata gcgtttagcg cggttaacta aattcgcctg
    21001 gtgcgccagt tgttcggcaa tggaacgatg ttgctgcgct tcatcggcaa acgaggcaac
    21061 attacagaat gtgcagccac cacgcccgat ggtaccgtca cggttagggc agctaaaacc
    21121 gccgtgcagc gtcagcttat gcaccttttg cccataacga cgggtgagat caccaccaaa
    21181 catattgact aatttctgta actgcataat ctgatagacc gcgccttgaa aagaggccaa
    21241 agcctgccat ttttagctca attcggcgat gacctggatc aatcgtcccg gcctgctttt
    21301 atcaactgca taatcaatca aaattaccga aatttcatgc ataatcacat aaatcacttt
    21361 tgcttatctt gagtcagatt tttttatctc cttatggatt ttaggcaaaa acagtagcat
    21421 gaaacgtcat taccaattaa ggcagtataa aatgctggtt ttctcgtcag ttcaaggcag
    21481 gataagggtt aacacgcctt tatgacagtc aggaattggc tgtttctcta acgactcccc
    21541 ttttgacctt aaatagaaaa tctattttaa tttcagttaa ttaataaaga attttgcgct
    21601 aaagcacatt tctgtaccaa taagcttgcc atttgacctg tatcagcttt cccgataagt
    21661 tggaaatccg ctggaagctt tctggatgag cagcctgctc atcatattta tgcagtaatt
    21721 gagatcccct cttcaccgta ttaaccgatg cgaaaaggac aacaaggggg cgaatgcgag
    21781 gcgcgcgtat gacacgcaac ccccgtcgcc acgctctttc tgtgcccgtg cgcagcggtt
    21841 cggaagtggg gttcccgcag agcctggggg aggttcacga tatgttgtac gataaatccc
    21901 ttgagaggga taactgtggt ttcggcctga tcgcccacat agaaggcgaa cctagccaca
    21961 aggtagtgcg tactgcaata cacgcactgg cccgcatgca gcaccgtggc gcgattctcg
    22021 ccgatggtaa aaccggtgac ggttgcggct tgctgttaca aaaaccggat cgcttttttc
    22081 gcatcgttgc gcaggagcgc ggctggcgtt tagcaaaaaa ctacgccgtc gggatgctct
    22141 tcctgaataa agatcctgaa ctcgctgctg ccgcacgccg catcgttgaa gaagagctgc
    22201 aacgcgaaac gttgtcgatt gtgggctggc gtgatgtccc cactaacgaa ggcgtgctgg
    22261 gtgaaatcgc cctctcctct ctgccacgca ttgagcaaat ttttgtgaac gccccggcag
    22321 gctggcgtcc gcgcgatatg gagcgccgtc tgtttatcgc ccgccgccgc attgaaaagc
    22381 gtctcgaagc cgacaaagac ttctacgtct gtagcctgtc gaatctggtg aacatctata
    22441 aaggtctgtg tatgccggcg gatctgccgc gcttctacct ggacctcgcg gacctgcgtc
    22501 tggaatcggc catttgcctg ttccaccagc gcttctccac taacaccgta ccgcgctggc
    22561 cgctggcgca gccgttccgc tacctggcgc ataacggtga aatcaacacc atcaccggca
    22621 accgccaatg ggcgcgtgcg cgtacctata aattccagac gccgctgatc cctgacctgc
    22681 acgacgccgc gccgttcgtc aacgaaaccg gttctgactc cagttccatg gataacatgc
    22741 tggaactcct gctggcaggc gggatggata tcatccgtgc catgcgtcta ttagtgccac
    22801 ctgcctggca gaacaacccg gatatggacc cggaactgcg cgccttcttt gactttaact
    22861 ccatgcatat ggagccgtgg gatggcccgg caggcatcgt gatgtccgat ggtcgttttg
    22921 ccgcctgtaa cctcgaccgt aacggtctgc gtccggcgcg ctacgtcatc accaaagata
    22981 agctcatcac ctgcgcctct gaagtcggta tctgggatta tcagcctgac gaagtggtcg
    23041 aaaaaggccg cgtggggcca ggcgaactga tggttatcga cacccgcagt gggcgcattc
    23101 tgcactcggc agaaactgat gacgatctga aaagccgcca tccatataaa gagtggatgg
    23161 agaaaaacgt ccgccgactg gtaccgtttg aagatctgcc cgatgaagaa gtgggtagcc
    23221 gcgaactgga cgacgacacg cttgccagct accagaaaca gtttaactac agcgcggaag
    23281 agctggactc cgtaattcgc gtactgggcg aaaacggtca ggaagcggtc ggttcgatgg
    23341 gcgacgacac cccattcgcc gtgctctcca gtcagccgcg cattatttac gattacttcc
    23401 gccagcaatt tgcccaggtc accaacccgc caatcgaccc actgcgtgaa gcgcatgtta
    23461 tgtcgctcgc caccagcatc gggcgtgaaa tgaacgtctt ctgtgaagca gagggccagg
    23521 cgcaccgttt aagttttaaa tcgccgattc tgctctactc cgatttcaaa cagctcacga
    23581 cgatgaaaga ggagcactac cgcgcagata cactggatat cacctttgac gtcaccaaaa
    23641 ccacgctcga agcgacggtc aaagagctgt gcgacaaagc cgaaaaaatg gtacgtagcg
    23701 gcactgtgct gctggtgctc tccgaccgga atatcgctaa agatcgcctg ccggttccgg
    23761 ctccgatggc ggttggcgcg atccagaccc gtctggtcga tcaaagcttg cgttgcgatg
    23821 ccaacatcat cgtcgaaacc gccagcgccc gcgatccgca ccatttcgcc gtgttgctgg
    23881 gcttcggtgc gacggctatt tatccgtacc ttgcctatga aacgctgggc cgcctggtag
    23941 acacccacgc gattgccaaa gattatcgta ccgtgatgct caactaccgt aacggcatca
    24001 acaaaggctt gtacaaaatc atgtccaaaa tgggcatctc caccatcgcc tcttaccgct
    24061 gctcgaaact gtttgaagcg gtcggtctgc acgatgatgt cgtaggcctg tgcttccagg
    24121 gcgctgttag ccgcattggc ggggcgagct ttgaagactt ccagcaggat ctgctgaatc
    24181 tgtcgaaacg tgcctggctg gcgcgcaagc ccatcagcca gggcggcctg ctgaaatacg
    24241 tgcacggcgg cgaataccac gcctacaacc cggacgtggt gcgcacactg cagcaggcgg
    24301 tacaaagcgg cgagtatagc gactatcagg aatacgcgaa gctggttaat gagcgtccgg
    24361 caaccacgct gcgcgatctg ctggcaatta cgccgggtga aaacgcagtc aacattgccg
    24421 atgttgaacc ggcaagcgaa ctgtttaaac gcttcgatac cgctgcgatg tctatcggcg
    24481 cgttaagccc ggaagcccac gaggcgctgg cggaagcgat gaacagcatc ggcggtaact
    24541 cgaactccgg tgaaggcggc gaagatccgg cgcgctacgg cactaacaaa gtgtcgcgca
    24601 tcaagcaggt ggcgtccggt cgcttcggtg ttactccggc gtacctggtc aatgccgacg
    24661 tcattcagat taaagtcgcc cagggcgcga agccgggcga aggcggtcaa ttgccgggtg
    24721 ataaagtcac tccttacatt gccaaactgc gctattcggt gcccggagtg acgctgatct
    24781 ccccgccgcc gcaccacgat atctactcta tcgaggattt agcgcagctg atattcgacc
    24841 tcaagcaggt caatccaaaa gcgatgatct ccgtgaagct ggtttccgaa ccgggcgtag
    24901 gcaccatcgc gactggcgtg gcaaaagctt atgccgattt aatcaccatc gcaggctatg
    24961 acggcggcac cggcgcaagt ccgctttcat cggtgaaata cgcaggctgt ccgtgggaac
    25021 tcggcctggt ggaaacccaa caggcgctgg ttgccaacgg tttacgtcat aaaattcgtt
    25081 tgcaggttga tggcggcctg aaaacgggcg ttgatatcat caaagcggcg attctcggcg
    25141 cagaaagctt cggcttcggc actggcccaa tggtcgcgct cggttgtaaa tatctgcgaa
    25201 tttgccacct gaacaactgc gccacgggtg tagcaactca ggatgacaaa ctgcgtaaga
    25261 atcactatca cggcctgccg ttcaaagtga cgaattactt tgagtttatc gcccgtgaaa
    25321 cccgcgagct gatggcacag ctgggcgtaa cacgtctggt ggatctgatt ggtcgcaccg
    25381 acctgctgaa agagctggac ggtttcaccg ccaaacagca gaaactggcg ctgtcgaagc
    25441 tgctggagac cgccgagcca catgcaggta aggcactcta ctgcaccgaa aacaacccgc
    25501 cgtttgataa cggcctgctg aacgcgcagt tgctgcaaca ggcaaaaccg tttgttgatg
    25561 aacgccagag caaaaccttc tggttcgata tccgcaacac cgaccgttct gtcggcgcat
    25621 cgctttcagg ctatatcgcc cagacgcacg gcgatcaggg tctggcagcc gatccgatca
    25681 aagcgtactt caacggcacc gcaggccaga gcttcggtgt gtggaacgcg ggcggcgtgg
    25741 aactgtacct gaccggtgat gccaacgact atgtcggtaa aggcatggcg ggcggcttaa
    25801 tcgccattcg ccctccggtt ggctcagcct tccgcagcca tgaagcaagt attatcggca
    25861 acacctgcct gtatggcgcg accggtggtc gtctgtatgc cgcaggccgc gcgggtgaac
    25921 gtttcggcgt gcggaactcc ggtgctatca ccgtggtaga aggcattggc gacaatggct
    25981 gtgaatatat gacgggcggt attgtctgta ttctgggtaa aaccggcgtg aatttcggcg
    26041 cgggtatgac cggaggcttc gcttacgttc tcgatgaaag cggcgatttc cgcaaacgcg
    26101 ttaacccgga actggtcgag gtcttaagcg ttgacgatct ggcgatccat gaagagcatc
    26161 tgcgcggtct tatcaccgag catgtgcagc ataccggttc ccagcgcggt gaagagattc
    26221 tggcgaactg gtcaaccttc gccacgaaat tcgcgctggt taaaccgaag tccagtgatg
    26281 taaaagcact gctgggtcac cgtagtcgta gcgcagcaga gttgcgcgtg caggcgcagt
    26341 aaggggtagc aacaatgagt cagaatgttt atcaatttat cgacctgcag cgcgttgatc
    26401 cgccaaagaa accgctgaag atccgcaaaa ttgagtttgt tgaaatttac gagccgtttt
    26461 ccgaaggcca ggccaaagcg caggctgacc gctgcctgtc gtgcggcaac ccatactgcg
    26521 agtggaaatg cccggtacac aactacatcc cgaactggct gaagctcgcc aacgaggggc
    26581 gtatttttga agcggcagaa ctgtcgcatc agaccaatac cctgccggaa gtgtgcggac
    26641 gtgtctgccc gcaagaccgt ctgtgcgaag gttcctgcac cctgaacgat gagttcggcg
    26701 cagtgaccat cggcaacatt gagcgctata tcaacgataa agcgttcgag atgggctggc
    26761 gtccggatat gtccggcgtg aaacaaaccg gtaaaaaagt ggcgattatc ggcgcaggcc
    26821 cggcgggtct ggcgtgcgcg gatgtcctga cgcgcaacgg cgtaaaagcg gtcgtcttcg
    26881 accgtcaccc ggaaattggc ggattgctga ccttcggtat tccggctttc aagctggaaa
    26941 aagaggtaat gacgcgccgc cgtgaaatct tcaccggcat gggtattgaa ttcaaactca
    27001 ataccgaagt gggccgcgac gtacagctgg acgatctgct gagtgattac gatgccgtgt
    27061 tccttggcgt cgggacttat cagtcaatgc gcggcgggct ggaaaacgaa gacgccgatg
    27121 gcgtgtacgc agcgctgccg ttccttatcg ccaacaccaa acagttaatg ggctttggcg
    27181 aaacccgcga agaaccgttc gtcagcatgg aaggcaaacg cgtggtggta cttggcggtg
    27241 gcgacactgc gatggactgc gtgcgtacgt ctgtacgcca gggagcgaag cacgttacct
    27301 gtgcctatcg tcgtgatgaa gagaacatgc caggttcccg ccgcgaagtg aaaaacgcgc
    27361 gagaagaagg tgtagagttc aaattcaatg tccagccact ggggattgaa gtgaacggta
    27421 acggcaaagt cagcggcgta aaaatggtac gtaccgaaat gggcgaaccg gatgccaaag
    27481 gccgtcgccg cgcggagatc gtggcaggtt ccgaacatat cgttccggca gatgcggtga
    27541 tcatggcgtt tggtttccgc ccacacagca tggaatggct ggcgaaacac agcgtcgagc
    27601 tggattcgca aggtcgcatc atcgccccag aaggcaacga caatgccttc cagaccagta
    27661 acccgaaaat ctttgctggc ggcgatatcg tccgtggttc cgatctggtc gttaccgcca
    27721 tcgccgaagg tcgcaaagcg gcagacggca ttatgaactg gctggaagtt taagctctgt
    27781 cccagcgccg cctcctcaga aaagagtcgg cgctgaaatt ctcctttcgt tctggaaagc
    27841 gcctcgcata atctgaagaa aaccaatgta atacgcacat ctggattgtt accggcagat
    27901 gcccggcgta tagctgattc cattattcaa cactgcgcca tattaaggga cagataatgg
    27961 aatcgctctc agaaggaacc acagcaggct accagcaaat ccacgacggc attattcatc
    28021 tggtcgatag cgcccgaacg gaaacggtac gtagcgttaa cgcgttaatg accgcgacgt
    28081 actgggaaat tggccgacga attgtcgaat ttgaacaagg tggcgaggcc agggctgcgt
    28141 atggtgcgca gctaatcaag cgactatcaa aggatttaag tctaaggtat aagcgtggat
    28201 tttccaccag gaatttatgg cagtttaaga atttttatat ttgttttcaa agaattgaaa
    28261 ttgtgcagac actgtctgca caattcaccc ccacaattct ccagactgtg tctggagaat
    28321 cttgccattt agcgcaactc gccaaatcct tccccctgcc ctggtccact tacgtccgtt
    28381 tgctttctgt taaaaaagct gatgctcgca gcttttatga gaaagagaca ctccgctgtg
    28441 gctggtctgc tcgtcagctc gaacgacaaa ttgcgaccca gttttatgag cggacactac
    28501 tgtcacatga caaatcagcc atgctgcaac aacacgctcc taccgagacg catattcttc
    28561 cgcagcaggc gatacgcgat ccctttgtgc tcgaatttct ggaattgaaa gatgaatact
    28621 cagaatccga ttttgaggag gcgctgatca accacctgat ggatttcatg ctggaacttg
    28681 gggatgattt tgcctttgtt ggtcgacagc gaaggttacg cattgatgac aactggtttc
    28741 gtgtcgatct gctgtttttc caccgccgtt tacgctgcct gctaatcgtc gatctaaaag
    28801 tgggcaaatt cagctatagc gatgccggac agatgaatat gtatctcaac tacgccaaag
    28861 agcactggac actaccggat gaaaatccgc ccatcggtct ggttctctgt gcagagaaag
    28921 gagccggcga agcgtattat gcactggctg gtttgcctaa caccgttctg gcgagcgaat
    28981 ataagatgca actacctgat gaaaaacgac tcgcggatga actcgttcga acacaggcgg
    29041 tgctagagga aggctataga cgccgttaat ttcagcggtt tcgaattctg cagacagtgt
    29101 ctgcagaatc tttaaaacga actaagtttt aagccattaa atcagcctta accttcacca
    29161 caaccttttt aatctcatca ggttcgccga caacgcatcc tggtttatgc ggttctcccg
    29221 gcataaacac ggcgaacatt cccggtttta agatgatggc ttgctcgttc tcaatggcgc
    29281 tgcaaagctg gtaatcatcc tcatggtgga actcttcaca ctgacgcgca gtgcctgccg
    29341 tgccaaacag aatccgttcc tcaccgttta acaacagctg gatatcaatg tattgctcgt
    29401 gcaattccgc ttttttctcg acgggcgatt gagtgttaaa cgtcatgaca ttcataaaga
    29461 tattgtcgcc ctgtaattcg taacgacccg gtgctttttc ttgcggcctg gcggctaagg
    29521 ctagtgttaa cgcgtcctgt aacgcaggat gcaatccggc agacggtaat gactgtactt
    29581 cacccatcat cataattttt ctccctgggc caacagcgca gccccaagta aacctgcatc
    29641 gtggcggtag tgcgccgcca gtaaatcaac atgaaatgcc gctggctcct gcgccagata
    29701 cgtttccacc agcgccagat acccctctgc cagaccaacg ctgccaccga ccaccacgca
    29761 ctggcaatca gttgtggctt taatatcagc gatcagcctt gcaagcgtac gtgcggaacg
    29821 gtgaatcagt tgctgcgcct gctcatcgcc ctgcccggca tgcgtgaaaa tagttttcgc
    29881 attcgcacca gccaactccc cttgcgccgc cgccgcaatg ccgcgaccag aagcaatcgc
    29941 ctccacgcaa cctgtgcgtc cacagccgca gactggacca tgcggatcgg caagcgtatg
    30001 cccgatgtgc cccgccagac cgccagggcc ggtaagcagt ttgccgccgc tcactacacc
    30061 gccgccgacg ccagtggaaa cggtgataaa caccatctcg gttacatcgc cttccagcgc
    30121 ctgatactct gcccatgctg cggcctgcgc gtcgttaatg gcaatggtcg gcaaattggt
    30181 aagttgttcc agcgttttga ctaacggaaa gtgtagtaat ccaccgagat tatgcggatt
    30241 aagcgccagc aaactgccgt cgcgaataat tccggttgaa gcgatggcaa cccgctgcgc
    30301 atgagcttgc aacggagaga ctaatgcgga taaggcatca cgcaaggctt ctggtgtctg
    30361 gctggctggc gtaggaagtt cacgacgatc gcggatctgc ccatcagcgc caatcagcgc
    30421 ggcggcaagt ttagtaccgc cgatatcaat cgccagtgtg gtcatagcac cgcctttttc
    30481 atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg taatggcaga accgaccgtc
    30541 accgcccacg cgccgtggcg catcgcatcc gccgcctgag caggcgtgtt gtaacgccct
    30601 tcggcaatca cccgacatcc ggcgtcgctc aacgttttca ccagcgccag atccggctct
    30661 tctggcgttt caggcgtggt atagccagaa agcgtagtgc caataatttc ggctcccagc
    30721 ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg tcatcgccag taaaccgtga
    30781 tggtgaatac gtgccagcaa cgtttcaaca ggcaccggac gcgggcggtc ggtgccgtca
    30841 atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcaa catcttcaat ataggccgtg
    30901 atgcgtaccg gagaatcctc cagatcgcgt ttcacaatcc cgataattgg cacgctcact
    30961 accgcacgcg tggcttgcag atttgccaca ccttcaatgc gaatggcaac cgcgcccgcc
    31021 tgctctgccg ctaatgccat ggcggcgacg atttcgggtt tatcgagcgg gctgtccgga
    31081 accggctggc aggagacaat caggccaccg ttagcagcga ttttttgatc cagttgtgca
    31141 agtaacgaca tacatcttcc cttagcgaaa ggcccggtac agagaccggg caacaggatt
    31201 aacttttggt tttgactaaa tcgtttttgg cgcggccaaa tggcacggca ccgctaaatg
    31261 gtttgccgtc gatagcgtca tgagtacgca atgcttccgg gcgcagccaa cgctgaacgc
    31321 gggaaggcat atccagacca atcagcagga tcaccacaaa tgtcagactg aacgagagcg
    31381 atgccagcgc agtacccaga tccagacgtt gagcgatcaa cgcgccgatg attggggcca
    31441 gtgcaccgcc caatgcgccg acgttgtagg taaagcccag gcccgctgca cgctggtcgg
    31501 tatcgaaata accgccaatc agtttcggta agatcccggc gatcccttgt ccaagcattt
    31561 gctggaagaa cagtaacaga ccgagcaccc agacgtttgc gccgccaatc gcaaataccg
    31621 gaataatcag cagctgcgag gccagcaggc tacaaacgta cgctttgcgg gttcccagcc
    31681 agtcaccgag gaagccacct acgcagcatc ccaccgccgc gccaaagcca ctaaagaaca
    31741 gcacattggc gacagtatgc gggttataag ccagatcggt tttcagatac gttggcagca
    31801 gcgcctgaat cggccatgag tagaggaaag caaacaacac gaccaccatc agcattacgc
    31861 ccgttggcca gcgtttgccc gtactttgca ccataaagct gataaagatt gcggcgcata
    31921 acagcccaag aacagcgacg atcgcggcat tttgcaggtt accggcgaag caaaaccaca
    31981 gcgcagtagc cgccgccagt gtcattacga tattggcaat gcgatgttca ccacggtaga
    32041 gaatatctac catcgtgcgt actggtgctt taccagcatg tttctctttc cagtcttccg
    32101 cttccgggat gtttttacgc agccagagag caaagatgat tggcaaaatg ccgataaaga
    32161 acagcgcacg ccagccccag accggaacca ccaggctata gacctgagcg gcaacgacgg
    32221 cccccacaga gaagccggaa atcagaaaac cactggcttt attacgcaga tgttttggcc
    32281 agctttcaat gacataggtg gcgctggaac cgtattcacc cgccatcccc atgccgatga
    32341 ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa gccgcaggcc agcgtcccgg
    32401 ccgagaagag aacgatgctg gtgaccattg ccagacgacg cccgtagcgg tcacccatag
    32461 cgccgagcat caggccgccg aaccagcgag agataaaggc tgcggagatc agacttgccg
    32521 cctgcaccgt cgtcagcccg aattcacctt gtacttcggt gagtaccagg gcgattaaaa
    32581 cgaaatcaaa accgtcaagc agatatccca accaggcagc ggaaaatgcc cgccattgtg
    32641 cacgattgag atggcgatac cacgggatat tctgggttga agtactcatt gtgagtctcc
    32701 cgcggtgggc gatgcccaca cgctttggta tgaaaattgt agggtacaga tgcgtttatt
    32761 tcccctcacc cggtaggggc gagcgagggg aaacaactca cccgcgctct tgcatcaact
    32821 gctgggccaa cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
    32881 ggcacagcgg cacagaaacg acatccatat aatgcaggac cgttttcagg ccgcggaata
    32941 cgcccgtttt gatcagtaaa tcaatgactt tattacattc ggtttgcagc ttctgcgcgg
    33001 tctggatatc gccttctttc agcgccttca caatcccctg ataacgccag cccatgatgt
    33061 tgtaggtact accgatacca ccatcagcgc ccgccagaag accagaggcg aagatttcgt
    33121 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
    33181 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaacaagt gtgttgattt
    33241 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaacc
    33301 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
    33361 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
    33421 gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
    33481 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
    33541 cttcgccggt cgaaccaccc acgtataaac cgtcaatgcc ctgctgaata ttgaactgaa
    33601 ccaggcgacg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
    33661 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
    33721 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
    33781 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
    33841 aaggcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
    33901 tcaggatcat gacgccggat cgcgtcaacg atcgcaatat gctgttgata gctaacgttg
    33961 ttatgctcgt gcagagcctg atcagccacg gttggacgtg cggcgatcag ccagtcgagc
    34021 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag aacgcggtgg
    34081 aaatcaacgt ctgagcgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
    34141 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
    34201 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccgcc aggatgagaa
    34261 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga aggacgtgag
    34321 acgcgggcac gttcgccgtt gtttatctgc accagacctt tgcgttttaa cgctgccagc
    34381 gcttcgcgca ccgaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
    34441 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
    34501 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
    34561 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
    34621 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcagggtt
    34681 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
    34741 atgtaacgca gataaaaaca tcccgattga attattcata agactattaa caagtattat
    34801 gaatattatg aatgtgttct tacaaaataa tcataagcgc atatttttta atgaaaaatc
    34861 acctcatcta caattaaaaa cacgacatcc gcaccataaa tagccttgca aaaaatataa
    34921 catcattgtt ttcaatctgc cgtttatggg attgaccgtt ttcttttgac acggagttca
    34981 acaatgttcg gcataattat atctgtcatc gtattaatta cgatgggcta tttgatcctg
    35041 aaaaactaca aacctcaggt ggtgctggct gccgcaggta tcttcctgat gatgtgcggt
    35101 gtctggttag ggttcggtgg tgtgctcgat cccgccaaaa gcagcggcta cttgatcgtc
    35161 gatatttata atgaaatcct gcgcatgctg tccaaccgca ttgccggatt ggggctgtcg
    35221 attatggcag tgggcggtta tgcccgctat atggagcgca taggggcaag tcgcgcgatg
    35281 gtgagcttgt taagccgccc gttaaaactc attcgctcgc cgtatattat tctgtcggca
    35341 acttacgtca tcggccaaat catggcgcag tttattacca gcgcctccgg tctgggtatg
    35401 ttgctgatgg tcaccttatt tccgacgctg gtgagtctgg gagtaagtcg cctctctgcg
    35461 gtggcggtta tcgcaaccac gatgtccatt gagtggggga ttctggaaac gaactccatt
    35521 tttgctgccc aggtcgcagg aatgaaaatt gccacttact tcttccacta ccagcttccg
    35581 gtcgcctctt gcgtcattat ctcggtggcg atctcccact ttttcgtgca acgcgctttc
    35641 gacaaaaaag ataaaaatat caatcacgaa caggcagagc aaaaagctct cgataatgtc
    35701 ccgccgctct attacgccat tttaccggtg atgccgttaa tcctgatgct cggctcgctg
    35761 ttccttgccc acgtcgggct gatgcagtca gaactgcatc tggtggtggt gatgttactg
    35821 agtttgacgg tgacgatgtt tgttgagttc ttccgcaagc ataacttgcg cgaaacaatg
    35881 gatgatgtgc aggcgttttt tgacggcatg ggtacgcagt ttgccaacgt ggtaacgctg
    35941 gtggtcgcgg gtgaaatatt tgcgaaaggc ttaacgacga ttggcactgt cgatgcggtt
    36001 atcaggggtg cggagcattc tggtctgggc ggtattggcg tgatgattat tatggcgctg
    36061 gtcattgcca tttgtgccat tgtgatgggc tcaggcaatg cgccgtttat gtcatttgcc
    36121 agtcttattc cgaatatcgc agccggacta catgtgccag cggttgtaat gattatgccg
    36181 atgcattttg ccacgacgct agcgcgcgct gtttcgccga ttactgcggt ggtggtcgtt
    36241 acgtcaggaa ttgcaggcgt ttcgcctttt gcggtagtga agcggacagc gatccccatg
    36301 gcagtcggtt tcgtggtgaa tatgattgcc acaatcacgc tattttatta agtcattaaa
    36361 aagacaaaac aggccgccag ggcctgtttt agtattactt cacaacgcgt aatgctggtc
    36421 gaccaccacg tggtggctgc ggtggttcat cgtcaggatg agtgtcatca tcgtgatctg
    36481 gcttgtcgcc atcaataacc gacataacgg tttcgttgtc tgccgatgcc tcttcatcat
    36541 tcatgatgct ggcatcttca tcgtaggcag cttcaggttc aaacatcgtg cctgcgccat
    36601 tttcacgggc gtagatagcc agcacggcag ccagcggcac agaaacctga cgcggaatgc
    36661 caccaaagcg cgcattaaag cgcacctcat cattcgccag ttccagattg ccgacagctc
    36721 gcggagcaat gttgagtacg atttgcccgt cacgcgcata ttccatagga acctgcacgc
    36781 cagggagcgt cacatccacc accaggtgcg gcgtgagctg gttatccagc aaccactcat
    36841 agaatgcacg cagcagatag ggacgacgtg gtgttagctg tgacaaatcc atacagatta
    36901 actccggccc agacgcattt cacgttcagc ttcagttaaa gaagcaagga aagagtcacg
    36961 ctcaaagacg cgggtcatat agcctttcag ctctttcgca cccgggccgc tgaactcgat
    37021 gcccagttgc ggcaaacgcc acagcagcgg agcaaggtag caatcgacca ggctgaactc
    37081 atcgctcagg aagtacggct tctgaccgaa gaccggcgca atcgccagca gttcttcgcg
    37141 cagttgctta cgtgcggcat ctgcttcaga agctgaaccg ttgatgatgg tgttcatcag
    37201 cgtgtaccag tctttttcga tgcgatgcat gtacagacgg ctttcaccgc gagctaccgg
    37261 gtaaacaggc atcagtggcg gatgcgggaa acgctcatcc agatattcca taatgatgcg
    37321 agattcccac agggtcagct cacgatccac cagggtcgga acgctctgat tcgggttgag
    37381 gtcaatcaga tcctgaggcg gattgtcctt ttccacgtgt tcgatctcga aacttacacc
    37441 tttctcagcc agcacaatgc ggacctgatg gctatagatg tcagtaggac cggaaaacag
    37501 cgtcattacc gaacgtttgt tggcagcgac agccatgaaa acctccaggt atagtcagaa
    37561 tttttactgc taccagccac caggtggcca gtcagaagtt gtgttaccca gtaaggaacg
    37621 actctctttg ttcgaaaatc aaacaaaaaa tgagcaatac ccgacatttg ggcagaaaat
    37681 tggatgatag tttaccagat tttgcgacct ttgtggtgag tcgatgccgg aaatggggaa
    37741 aaagagatgc gctttagtct gaaatagttg acttagtccc ttattggcga tgtggttttt
    37801 gttttgcctg tctgtcagac ggcagcaaaa agcaactttc cattttttac gctgattcag
    37861 aatttagcca taaaaaaacc cgccgaagcg ggttttttcg caaattgttt tctgccggag
    37921 cagaagccaa ttaacgtttg gagaactgcg gacgacgacg tgctttacgc agaccgactt
    37981 tcttacgttc aacctgacga gcgtcacgag taacgaagcc agctttacgc agttcagaac
    38041 gcagggactc gtcgtattcc atcagagcgc gggtgatacc gtgacggatc gcaccagcct
    38101 gaccagagat accaccacct ttaacggtga tgtacaggtc cagtttctca accatgtcga
    38161 ccagttccag cggctgacga actaccatgc gggcagtttc acgaccgaag tactgttcca
    38221 gagaacgttg gttgattacg attttaccgt tgcccggttt gatgaaaacg cgagctgcgg
    38281 aacttttgcg gcgaccagtg ccgtagtatt gattttcagc cattgcctat aatcccgatt
    38341 agatgtcaag aacttgcggt tgctgtgccg cgtggttgtg ctcgttaccc gcgtaaactt
    38401 tcagtttacg gaacatagca cgacccagcg ggccttttgg caacatgcct ttaaccgcga
    38461 tttcaatcac acgctcagga cggcgagcaa tcatctcttc aaaggtcgct tgtttgatac
    38521 caccgatgtg gccggtgtgg tgatagtaca ctttgtcagt acgcttgttg ccggttacag
    38581 caactttgtc agcgttcaga acgatgatgt aatcaccggt atctacgtgc ggagtgtatt
    38641 ccgctttgtg cttaccgcgc aggcgacgag ccagttcagt agccagacgg cccagagttt
    38701 taccggtcgc gtcaacaaca taccagtcgc gttttacggt ttctggttta gctgtaaaag
    38761 ttttcattaa aagcttaccc aataaatagt tacacgttgg tgaacaccca aacgtcttca
    38821 attgttgagg ttcacacgac aaagtccggc aaacctaccc cttcgaatag cctatgccag
    38881 cacacaaaaa gttttgggaa aaaaactttc ttgtaacgtg gggtcgcagg attatagaga
    38941 agtcggggtc aaagatcgac ccctttttgt gatttgtggc aggttttaac ccgccaaatg
    39001 ctcgcgcttc agatactctt cgctttgcat ctcttgcaga cgtgacaggc aacgctggaa
    39061 ctcaaacttc agccgatcgc cctgataaat ttcatacagc ggcacttctg cactcaccac
    39121 taatttgaca tggcgctcgt aaaactcatc caccagcgca ataaagcgcc gcgcttcgct
    39181 ctccatcaac cgcgtcataa gtggtacatc aaacaacatg actgtgtgaa agaggcgtga
    39241 gagcgcaata tagtcatgct gactgcgggc gtcgacgcac agcgtagtaa aagagaccgc
    39301 cagcgtctgg ttctcgacgc ccattgtcgc caatggccga tggttgattt ctaacgtcgg
    39361 tgaattttct cgtttcgccc ccgccagcgc caaccatagt ttatccattt gcgcccgggt
    39421 ttcatcgttg aggggcgaaa gccacagatg cgcctgagtg agtgtacgca gtcgataatc
    39481 aacaccagcg tccacgttca ttacatcaca atactgttta atggcatcga ttgcaggcaa
    39541 aaaacgcgca cgttgtaggc catttcgata aagttcgtcc ggcggaatat ttgacgtcgc
    39601 taccagggta ataccgcggg cgaacagggc tttcatcaga ccgccaagta gcatggcatc
    39661 ggtaatatca gaaacaaaaa attcgtcaaa acagagcacg tcagtttctg ctttaaagcg
    39721 atcggcaata atttccagcg gatcggtctg cccctgcaaa gcagttagct cttcatgtac
    39781 ccgcagcata aaacggtgaa agtgcagacg ctgtttccgc tctcccggca ggctttgata
    39841 gaaaaggtcc atcagccagg ttttcccgcg tcctacaccg ccccacatat ataagccacg
    39901 cactggcgta tgctttgtgt cttcgcgttt accccacagt ttaccgaccc gcgccattag
    39961 cccactcgtc ctgggggctg gaggcgtgct attgatgagt tcctgataaa taatttccag
    40021 gcggctgacg gcctcttttt gaacgtcgtc gggttgatgg ctgccttcat tgagcgcctt
    40081 caggtattgc gatgttgggg taatgctttg catgatctta ttgttattcc ttgaataatc
    40141 ggtgcgccgt tgttcacggt tgacgaaaaa aaggccgttc tacactacgc gatatgcagt
    40201 cgggattcca cttctgtgga attaacggtt atagtggcat aatcagccgc aggcatggag
    40261 cctgaagcca acaccctacg gaaacaaaag acaacgggag atgttcatga cctgggaata
    40321 tgcgctaatt gggttagtcg tcggcatcat tattggtgct gtggccatgc gttttggtaa
    40381 tcgtaaacta cgccagcaac aggcgttgca gtacgaactg gaaaagaata aagctgaact
    40441 ggacgagtat cgcgaagagc tggttagcca ctttgcccgc agcgcggaat tactggatac
    40501 catggcgcac gactatcgcc agctgtatca gcatatggcg aaaagctcca gcagcctgct
    40561 gccggaactg tctgctgaag caaacccgtt ccgtaatcgt ctggcagagt ctgaagccag
    40621 caacgatcag gcaccggtgc agatgcctcg cgactattct gaaggtgcat ccggcctgct
    40681 gcgtactggc gcgaagcgcg actaatttat ttttcgggcg cagccattgc gccctcctct
    40741 tctctccctc cccgactatc atttaatctg gtgtctcatt gttagccgtc tgaaaattca
    40801 ataacatcaa actgttttga atctcttttc ttatcattca ggtacgagag caggaataat
    40861 gaaaaaacaa acccagctgt tgagtgcatt agcgttaagt gtcgggttaa ctctctcggc
    40921 gtcatttcag gccgtcgcgt cgattccagg ccaggttgcc gatcaggccc ccctccccag
    40981 tctggctcca atgctggaaa aagtgcttcc ggcagtggtg agcgtacgag tggaaggaac
    41041 ggccagtcag ggacagaaaa tcccggaaga attcaaaaag ttttttggtg atgatttacc
    41101 ggatcaacct gcacaaccct tcgaaggttt aggctccggt gtcatcatca acgccagtaa
    41161 aggctatgtg ctgactaaca accatgtgat taatcaggca cagaaaatca gtattcagct
    41221 caatgatggg cgcgagtttg atgcaaaact gattggtagc gatgaccaga gcgatatcgc
    41281 cctgttgcaa attcaaaacc cgagcaaatt aacgcaaatc gctattgccg actccgataa
    41341 attgcgcgtc ggtgattttg ccgtggcggt cggtaaccca tttggccttg ggcaaaccgc
    41401 cacctctggc attgtgtcag cattaggccg cagtgggttg aatcttgaag gtctggaaaa
    41461 ctttatccag acagatgctt ccattaaccg cggtaactcc ggcggtgcac tgttaaatct
    41521 taacggtgag ttaattggca tcaacacggc aatccttgcg cctggtggcg ggagcgtcgg
    41581 gattggattt gccatcccca gtaatatggc gcgaacactg gcgcagcagc ttatcgactt
    41641 tggtgaaatc aaacgcggtc tgttaggcat caaaggcaca gagatgagtg ccgatatcgc
    41701 caaagccttc aaccttgacg tgcagcgtgg cgcgtttgtc agcgaagtgt tgccaggttc
    41761 tggctcggcg aaagcgggcg tcaaagcggg cgatattatt accagcctca acggcaaacc
    41821 gctgaatagc tttgctgagt tgcgctctcg tatcgcaacc accgagccgg gcacgaaagt
    41881 gaagcttggc ctgctgcgta acggcaaacc actggaagta gaagtgacgc ttgataccag
    41941 cacctcttcg tcggccagcg ctgaaatgat cacgccagcg cttgaaggtg caacgttgag
    42001 cgatggtcag ctaaaagatg gcggcaaagg tattaagatc gatgaggttg tcaaaggaag
    42061 cccagctgct caggctggct tgcaaaaaga cgatgtgatc attggcgtca accgcgatcg
    42121 ggtgaactcg attgctgaaa tgcgtaaagt tctggcggca aaaccggcca tcatcgccct
    42181 gcaaattgta cgcggcaatg aaagcatcta tctgctgatg cgttaatgtc gtaaaccggg
    42241 catcaggctg acgtgtgatg tccggttaac tcgtggtatg ctgctgccgt tccctttttt
    42301 aatgacgcct ccatcatgtt tgtgaagctc ttacgttccg ttgcgattgg attaattgtc
    42361 ggcgctattc tgctggttgc catgccttcg ctgcgcagcc ttaacccgct ttccactccg
    42421 caatttgaca gtaccgatga gacgcctgcc agctataatc tggcggttcg ccgcgccgcg
    42481 ccagcggtgg ttaacgttta caaccgtggt ttgaacacca actctcacaa ccagcttgag
    42541 atccgcaccc tgggatccgg tgtaatcatg gatcaacgcg gttatatcat caccaataaa
    42601 cacgtcatca acgacgccga tcagatcatc gtcgccttac aggatggacg tgtatttgaa
    42661 gcattgctgg tgggatctga ctctctaacc gatctggcgg tacttaaaat taatgccact
    42721 ggcggtttac ctaccattcc aatcaatgca cgtcgcgtac cgcacattgg cgacgtggta
    42781 ctggcgatcg gtaacccgta caacctcggg cagaccatta cccaggggat tattagtgcc
    42841 acgggtcgaa tcggtctgaa cccgaccggg cggcaaaact tcctgcaaac agatgcttcc
    42901 attaaccacg gtaactctgg cggcgcgctg gtgaactcgc tgggcgaact gatgggcatt
    42961 aacacgctgt cgtttgataa gagtaacgat ggcgaaacgc cggaaggtat cggctttgcg
    43021 attcctttcc agttagcaac caaaattatg gataaactga tccgcgatgg tcgcgtgatc
    43081 cgcggctaca ttggtatcgg cggacgtgag atcgcaccac tgcacgcgca gggcggtggt
    43141 atagatcaac tgcaagggat cgtggttaat gaagtgtcac ctgacggccc ggcggcgaat
    43201 gcgggtattc aggtcaatga tttgattatt tcggtggata acaaaccggc catctctgct
    43261 ctggagacga tggatcaggt ggcggaaatt cgccctggct cggtgatccc ggttgtagtg
    43321 atgcgtgatg ataagcagtt aacgctgcag gtcaccattc aggaatatcc ggcaaccaat
    43381 taagtcgtgc gctcaaaaca aaaaaccgga gtctgtgctc cggtttttta ttatccgcta
    43441 atcaattact tattaacgaa ctcttcgccc agggcgatat ctttcttcag cgtatccagc
    43501 ataccttcca gcgcgttctt ttcaaatgcg ctcagagtgc cgatagattt acgctcttcc
    43561 acgccgtttt tacccagcag cagcggttga gagaagaaac gtgcgtactg accgtcacct
    43621 tcaacgtagg cacattcgac aacgccttgt tcaccttgca gtgcgcgaac cagagacagg
    43681 ccaaaacgtg cagctgcctg gcccatagac agggttgcag acccgccacc ggctttcgct
    43741 tcaaccacct cagtacccgc gttctggata cgtttggtca gatcagccac ttcctgctcg
    43801 gtaaagctaa cgccaggaac ctgtgacagc agcggcagaa tggtaacacc agagtgaccg
    43861 ccaataaccg gcacttcaac ttcgcctggc tgtttgcctt tcagttccgc aacaaaggtg
    43921 ttggaacgaa tgatatccag cgtggtaacg ccgaacagtt tgtttttgtc ataaacaccg
    43981 gcttttttca gcacttcagc agcaatcgca actgtggtgt taaccgggtt agtgataata
    44041 ccaatgcacg ctttcgggca ggttttcgca acttgctgta ccaggttttt cacgatgccg
    44101 gcgttaacgt taaacaggtc ggaacgatcc atacccggtt tacgcgctac acctgcagag
    44161 ataagaacga catctgcgcc ttccagcgcc ggagtcgcat cttcaccaga aaaacctttg
    44221 attttcacag cagtagggat atggctcaga tcgacagcca caccgggagt cactggagcg
    44281 atatcataca gagagagttc tgaacctgaa ggcagttggg tttttaacag tagtgcaagc
    44341 gcctggccaa taccgccagc agcgccgagg actgcgactt tcatcctaaa ctccttatta
    44401 tattgataaa ctaagatatg ttgctccgct gccgcgacct taatccacaa aattgccgtt
    44461 tacaatgacc acatctcaag aatgtgtagt cacgcaagtt tagcgtttat gcatttaatt
    44521 gccgtaatca ggaacctaac ttacgtaatt aacagtcgct tatgcattag cgcaacattc
    44581 caacaggtgg tgacaatata ccctaccgtt cagccaaaac aacatcaatt tgataacaat
    44641 taatttactt ttaagcagaa tttgcatgcc gtgacgcagg catgtttctc aataacgaaa
    44701 tttgataaaa tcccgctctt tcataacatt atttcagcct tcttcagggc tgactgtttg
    44761 cataaaaatt catctgtatg cacaataatg ttgtatcaac caccatatcg ggtgacttat
    44821 gcgaagctcg gctaagcaag aagaactagt taaagcattt aaagcattac ttaaagaaga
    44881 gaaatttagc tcccagggcg aaatcgtcgc cgcgttgcag gagcaaggct ttgacaatat
    44941 taatcagtct aaagtctcgc ggatgttgac caagtttggt gctgtacgta cacgcaatgc
    45001 caaaatggaa atggtttact gcctgccagc tgaactgggt gtaccaacta cctccagtcc
    45061 attgaagaat ctggtgctgg atatcgacta caacgatgca gttgtcgtga ttcataccag
    45121 ccctggcgcg gcgcagttaa ttgctcgcct gctggactca ctgggcaaag cagaaggtat
    45181 tctgggcacc atcgctggcg atgacaccat ctttactacc cctgctaacg gtttcaccgt
    45241 caaagacctg tacgaagcga ttttagagct gttcgaccag gagctttaat ctctgccccg
    45301 tcgcttctgg cggcggggaa aatgttgctt atccctctca accccctgct ttcctctccg
    45361 attaatttaa cgaatagtgc gttttactgc gacatgtcat tcacacaatg aatacatacg
    45421 gtaaaaaaag cacattatgc aaaattcatt atctaattga aaacaataga attaataata
    45481 aataaccgtg tttttaattc ttttttgtta ttaaaactca catttttaac acttagtatc
    45541 aactgaaaca gttagcgtgg tattaattag ttcaataatt agtgtatact tgattttgtg
    45601 atatgggtca cgaaacaaag gcccagctaa aagattatgt cgaggtaaaa atcatgaaaa
    45661 tcaaaaccac tgttgctgca ttaagcgtac tttctgttct ctctttcggt gcattcgctg
    45721 ccgactccat tgatgctgca caagcacaaa atcgtgaagc aatcgggacc gtatccgtaa
    45781 gtggtgtggc gtcttcgcca atggatatgc gtgaaatgct gaacaaaaaa gcggaagaga
    45841 aaggcgcaac ggcctaccag attactgaag ctcgtagcgg tgacacctgg cacgctacgg
    45901 ctgaactgta caaataaacc ctcatcgtct tgtccgacga tattgccccc ggttcggggg
    45961 cttttttttg cgctaatgac gaacattaaa acgcaaatgc ccttccagct cttcctctgc
    46021 ctcatcaaac agcaatatta acgcgccaaa acggcgacgc gttttctctc ccagatgaac
    46081 aaattcaatc tcaagtggta gcggcaggac atcgttcatt aacacatccc atagtgagtc
    46141 gagatcgcgt accttatctt tcgccagacc aaaggcttgg ctgaagtcgc gataaaaatc
    46201 ctcctgactc tcaatctcat caaaatcaaa ggtataaata ttcatctgtt gccaccgtca
    46261 cgtttcgcgg cgagttctgc cgttcatcgc taagtatagc catgaaaaaa ccgacgcttt
    46321 tggcgtcggt tttgacttaa ctatcggtca acgcatgttg atacttatgg agcatccccg
    46381 ccagccgatg aaccggttcc gtcacctgcg gtggcgcttg ccagatgcgt agcttttcct
    46441 gatagatttc aagttcttcc agcaactggc caaagtaccg acgacgttta tcatcgctac
    46501 gggcagatat cacatggtct gctgtgcggc gcatttgtcg gtgaaacgcc gataaatcct
    46561 cgttaaccgg gatcggcgca tcacgcaggc gctggtgcgc gataatcatc gtcagcgcca
    46621 ggcgaaattt cggcaaatcc cctgggaact tattcatcag caaaaacagc tgctgataaa
    46681 gtgccgggag gtggttctct ttacgacgtg ccacattggt agtcatcgcg gaaacagcag
    46741 cagaaacgaa ctgattaagc agtacacgtc cagtcctgtc gcgcgattta tcccgcacca
    46801 gcaaaataac ggtgaacgcg agcacacagc cgacgatttg ccctaatgcg ctgtcgagaa
    46861 actgactgaa atggaaagtc atcgggttat ccagcacgat aatatttatg gtgctggcca
    46921 gcgcccccat cgagcccagt cgccgtttct gtacttctat accgaggaag aatcccagca
    46981 ctgccaggct aatacacagc agcaacatgc tctgttgagt attagggata atcaccaaaa
    47041 agtagagcag ccctaacggc agcgctgcca gcgtcccgta gataaagtcg atcgccacca
    47101 tgcgtggatt cggcaaacgc attgccagtg acgtcactac cgcaatcatc accattgcac
    47161 cactgccgga agtccagccc gtccacagcc agaaaagcgt ccccagaatg caggaaagtg
    47221 tggttcgcca gaagttaacc atcgcatgat gacgttcggc tgactctact ttgacttccg
    47281 gttcgccttg caggatctct tcttcggtgg cgttgatttt tgtgttactg ataacgccgc
    47341 gcttgagaag ctgataacgc gttgccgccg cgacccagct ataaatggtg acaggcgttt
    47401 cccgttcccc agtccaggcg ataactctcc gcaggcgttt gagctgcttg tggacgtcct
    47461 gcgcggtttc taccggcgtg tcaaaaaatt cgcggaaagt atcagtgatc aattccgggc
    47521 gcgtattctg aataagataa gtttcgcagg attgtgtaat cagcgtcagc gatagcgtat
    47581 taatcgcttt taaacgtcga ttggcccgcg cccagcggga agattccata ttcaggttgc
    47641 tgcgcatacc ttgtaacgcc gtggtgcgtc gcaccagatc gccccaggct ttatcgacaa
    47701 cttcaccatc gccatgcttg atacagagtt gcattaattg atattgcgcg accagcaaac
    47761 tttccagctc tcgatccact tcttgtttga tcgatcgcgg agaaaagagc aaatccgcca
    47821 taatggcaca cacaataccg ataacgatct cgctacaacg ttcgacggca aactgcggcg
    47881 taagcaatgg ttccggctga atggtgatca caatgatcag cgcggtataa ccggccagcc
    47941 cccacgcata cgagttttct attcgtacca gcgaggatat ccaggtacaa aaaccggccc
    48001 agatacagca caccagaatc atcaataatg gtgcacggat catcgcaata atgatcacca
    48061 gtccggcaat acagccaata aatgtgccga tgatgcgcaa aaagccacga tagcgaatag
    48121 cgccagaata cggttcacct cccgcagcaa aggccggacc ggcggcaaca atcgccgctg
    48181 tcagtactgc ccagcgtggc gtttccagct ggaagtgaaa gccaacaaac agcgccagta
    48241 caatggcggt cgccagtttt accgcaaagc gaatatgttg gttagcaatg gagaaaatac
    48301 ccatcgtgat taaccaaact cacgcaggcg atgggccatt ttacggaaga acgaatcctg
    48361 gctttcgtcg cgatcttgtt tgccagtgac caccactgta gcagtggtgc ccgcaggcca
    48421 gatgttctct tgctggttgt cgagacgaat acgaaccgga acacgttgcg ccaaacgcac
    48481 ccattcaagg ttagagtcga tggtcgccat ccctttgtcg tcacgcgtgc tgctggcgtt
    48541 ggtgacccct gcggcaacac tatcaacagt cccttttagc actttgttac tgccaagcgg
    48601 cgtgatctct gcacgatacc cagggcgcac cccttccagc ttagtttctt ccatataggc
    48661 cagtacatag aaggagttct gtttcaccag cgcaaccgcc gttgatccac gggtaataaa
    48721 ctcaccggta tagacgttga ggttggtcac ccagccatct gctggcgcgc ggatcaccgt
    48781 gcgttcaaga tccagttttg ccagatcgcg ggtcgcctgc gctttcgcta actgatgcag
    48841 aactgtttgt agtacgttgt tggcctggtc gatctcttcg cgggacatcg cctgcacacc
    48901 gagacggtta cgacgtcctg cctcctggcg tttctcctgt gccagtacct gataataagc
    48961 aacatcggct tgcgcttcct caagcgcctt ttgatagcgc ggctggtcga tggtgaacag
    49021 tacctgtcct tttttcacca gctggttatc atgaacattc acctgggtga taagtccaga
    49081 aacgtccggc gcgatcgcaa cgacgtcagc gctaaagcgc gcgtcacgcg tccacgggga
    49141 ttcggtgtaa tagacccagg cattaaaaat tgcgatgaag gccagaatga ctaatacgac
    49201 cgtgatggcc gtacgggaga attttcttat tagtgttttc acttcaacct caaacgaaca
    49261 gtcgcgatat caaataaaac aagcagcaat agagcgcggt gttgaacaac gccggatgcc
    49321 agacaaagtc gtagatacct gttggcacaa gtacccggcg caccagccag aaaatcgcca
    49381 gtgataaaag caattcaaaa aatatcggtg ggaaggacag cccaaacacc acgataacgg
    49441 gaaacagact catgttgacc ttggttgtat agagagagca ggcgttatta ttttcagcat
    49501 ctgtcgccgc agagaagggc atggaaagcc gggcgagagc aacattgctg tagattgata
    49561 tttaatatat tagcgtaact gttatgctgt tatctatatt atgtgatcta aatcactttt
    49621 aagtcagagt gaataatgga acgactaaaa cgcatgtcgg tgtttgccaa agtagttgaa
    49681 tttggctctt ttaccgccgc cgccagacag ctacagatga gcgtttcgtc catcagtcag
    49741 acggtgtcaa aactggaaga tgagttgcag gtaaagctgt taaaccgtag cacacgcagc
    49801 attggcctga ccgaagccgg tagaatttac taccagggct gccgtcggat gctccatgaa
    49861 gtgcaggatg ttcatgagca actgtatgcc ttcaataaca cccccatcgg gacgctgcgc
    49921 attggctgtt cttcaactat ggcacaaaat gttctcgccg ggctgacagc aaaaatgctg
    49981 aaagaatacc caggtttgag cgtcaatctg gttaccggaa ttcctgcccc cgacctgatt
    50041 gccgacggtc tggatgtggt gatccgcgtc ggcgcgttgc aggattccag cctgttttcc
    50101 cgccgtctgg gcgcgatgcc aatggtggtg tgcgccgcga aaagctatct cacacaatac
    50161 ggcataccgg aaaaacccgc cgatttaagt agtcattcat ggcttgaata cagcgtgcga
    50221 cccgacaatg aatttgaact gatcgcaccg gaagggatct cgactcgcct gatcccgcaa
    50281 gggagatttg tgactaatga tccgatgacg ctggtgcgct ggctgactgc aggcgctggg
    50341 atcgcctatg tgccgctgat gtgggtgatc aacgagatca atcgcgggga gctggagatc
    50401 ctgctgccgc gttaccagtc agatccacgc ccggtttatg cgttgtatac cgaaaaagat
    50461 aagctgccgc tgaaggtaca ggtcgtgatc aactcactga cggattattt tgttgaggtc
    50521 ggtaaattgt ttcaggaaat gcacgggcgc gggaaagaga agtaattcat attgtactgt
    50581 tacgttgtac aaacctgtgc caatgggttc ccctcaccct aaccccagag ggggagggga
    50641 ccgttcgtgc acgtagaagg attaattatc cttctgaaaa tactgaaatt attacgcggt
    50701 accgccaaca gtcaggttat caactttcag cgttggctgg cccacgccaa ccggcaaact
    50761 ttgcccttct ttaccgcaga cacccacgcc gttatccagt ttcaggtcgt tgccaaccat
    50821 cgaaatctgc tgcatggttt cgataccgga accaatcaac gtcgcgcctt tcaccggctt
    50881 cgttacttta ccgttttcaa tcagatacgc ttctgaagtg gagaaaacga atttgccgga
    50941 ggtgatatcc acctgaccgc caccaaagtt cggtgcatag ataccgtact caacggattc
    51001 aataatttcc tgcggggtcg atttacccgg cagcatatag gtgttggtca tacgcggcat
    51061 cggcagatga gcgtaggatt cacggcgacc gttgccagtc ggcgtcatcc ccatcaaacg
    51121 cgcgttgagt ttatcctgca tgtagccttt caggatgcca ttctcaatca gcacgttgta
    51181 ctggcctggc gtaccttcgt catcaatcgc caccgaacca cggcgatcga ccatcgtgcc
    51241 gtcatcaacc acggtacaca gttcagaagc caccagctcc ccgacctgtc cactaaatac
    51301 tgaagtgcca cggcggttga agtcgccttc cagaccgtgc cccaccgctt catgcaacag
    51361 cacgcccggc caacctgcgc caagtactac cggcatggtg cccgctggtg cagcaacggc
    51421 agaaagattg accagcgcca tacgcacagc ttcttttgcc catgcatccg cacggacttc
    51481 accgtcgaga tcggcaagga agaactcata accaaaacga ccgccgccgc cactggcacc
    51541 gcgttcgcgt ttgccatctt cttcgaccag aacgctcacg gaaagacgca ccagcggacg
    51601 gacatccgcc gctagcgtgc cgtcggtggc cgcaaccaga attaattcat agacaccact
    51661 gaggctggca gtcacttcct gtacgcgctt gtccgcttcg cgggcaacct tatcgacgcg
    51721 acgcaggata tccagcttct cttcacggct catgctttgc agcggatcta ccgaggtata
    51781 caacgggcta tgctctaccg cgcccagcgt ctgtactttg ccatcaccac tatcacggac
    51841 gatggtgcgc gccgcttgcg cactctgttc cagcgccagc aggctgattt ggtctgcgta
    51901 agcaaatccg gttttttcac cgctgattgc acgcacacca acgccctggt cgatgttgta
    51961 agaaccatct ttaataatgc ggtcttctaa aacccaggat tcgtgatagc tcgactgaaa
    52021 atagagatcg ccataatcaa ggcgacgttc ggccagttga ccgaggatcg cgaacaagtc
    52081 ctgatgtttc aggccgttcg ccgctagcaa ttgttcactt accaggttaa gactcatcgt
    52141 ttttgctact cgttagttac tgcagtagag gattttttac ggctgccgga tgcggcgtga
    52201 acgccctgtc cggcctacgg ttatgttctg gctcgttggc ctttggcaac gattatcata
    52261 tgagattggg gcaattacgc gccctcgtca aatcattgcg ctttttcttt acgcggttgg
    52321 cgcaacactt cgttgatttg cggatcgtcc agcggacccg aaatgtgata gcgcaaaatg
    52381 gagactttgc tccacagcgg ccccagcact ttactggctg caaacactgc cgcgccaaca
    52441 atggggttaa ccgcaaaagc cgcagccacg cccaccgtcg cagaaatctc tggtgcgaca
    52501 accgcttcca tattcaggtc gcgacgcacc agatttaccg accctttcat ggcaatgtcc
    52561 gcttccaggc catccaccag cgtgtcgtcg gtgtgcataa cgccgtcttt aatccacgcg
    52621 gtgctgcgaa tggagtcaaa atagaaccct tcaccaaagg tgtctctgaa atcaaaacgc
    52681 agcttacgca tcagggcatc aacgctcaat aagcgcagca attgcccggc atgtccggta
    52741 ttgatttcgg taatttcgcc tttgcccagt tgagtatgaa tgatgccatt caacgtcgcc
    52801 tcatctggtt gccagggtgc tttgcgccag tgtaaatcgt aatccacatt aaatgacgac
    52861 tggcgtatgg gcgtcgtgac accaaaaaat tctgcggcgg catcaatttt ctgcccacgc
    52921 agttttcctt tcagcgaggt gcgttcattt cccggattgt taacccattc accatcggca
    52981 gtgagccgcg aaaaaccagt atcaatcagt ccattagtca gcgttaatgt attgccagaa
    53041 atggtgatat cactgtcaat gcgcccgaat ttttgccccc agaaccagca ctctgcgcat
    53101 cgtatttggg cgtccggcca gccacggaag ttaatgcgct ccgttgtcgg gaaaggtgat
    53161 gactgtgttg aatcaccacg agttttcgcc acgctcgggt tgtaataaag atatttgata
    53221 ttcgccagcc acggcgcgtt attacgcatc gccagcgtgg cgttgatttc acgcccttgc
    53281 gcctcaacct gggtgccatt tgccgtcggt tgcgaaacaa tactcaggtt attccattgc
    53341 tgatttccca gcgacaacat aggcgtacgt aacgttatgt gttgtgggaa actcgctgca
    53401 ccaccgacac tctccgccgc gcctttctgg aacagggcca gccactcggc accattcatc
    53461 ggcggcatat tgagttcaac gccactttgt tccggcaacg gcgggagcgt tttactgtct
    53521 gccgcccaaa tagcacgatc gagcgtcaac ttttgaccga gcaaccagcg gctattgaaa
    53581 tggttatccg cacctgcctg tccggttaat tcaaagctgt tgagattgcc atcaacctta
    53641 acgtttaccg ctagtggttc acccgcaggt ttggctaacg gtgaaggtaa gtgactgctc
    53701 acattcttca ggtcgccgtt cagctctacg ttataggtcg cgccagcatg ataaggcaga
    53761 tcaatgcccg ctttaccatc ccacgccacg ctgccactta atgcttcgtt caccgctgca
    53821 ggcagaacgc cggttttcgc tggttgccag ttaccgttga gattcaccgc aacctggtag
    53881 gcttttgccc cttctttggt ggaaaaatcc acgttcaacg gctgattaaa ccagcttgct
    53941 gtcagtggtt cactttgcaa atcgccattg ataaagctga atttaccgct caaatttttc
    54001 agggtgctgt cgagtggttt gataaacaga ctgttattac gcagcgtcac ttcacctttc
    54061 gcggttacca gttcgccgtt cagcggaata tcgagatgta agcgagcatt cacatcgccg
    54121 tcgagctgga gttcttgcag ggttgcaccc agagaatctt tcagcggtgt ctcatcaaag
    54181 taagggccga cggctttacc cggaccttta atgtcagcgt caatcagcag tttttctttc
    54241 gagtagtcag ggatcactgc ggtgagatta ctcgcgcgca cgccgcccag attaacgcca
    54301 tcggttttca tccataaacc gtcgttaata aagtccagtt caatatcaag gttagttaat
    54361 gcaggccagt ccggctggaa ggcaaacttc gcgttgcgca gcggcaccag cacttcaaac
    54421 tgaccttcgt tgtgtttata ggggaagagt tgcggattgc caccataaac cagcgtcgcg
    54481 ttatccgcta caccgccctg aatcgcgcca cttaagtaat caaccaggtc tttacccatc
    54541 aagttttccg ggaaatagcg ccaggcttgt gaaccatcat cggtactgat gccagccaga
    54601 atacccagcc agggttcatc gttagcaggt tgcaggtaac gaaaaccgcc gcgcgcatgg
    54661 acggctttgg ctttaacgtc aatattacgc ccatccagct ggaaaccttt gtcattgttc
    54721 agccagctta tcgttgcctg gccgtcggcg atttccagtg gcgcacggaa taccgtttcg
    54781 taaggcatct ttgcctgctt catcgacgcg gtaagcaaac cattttctac gctgccggaa
    54841 agcgtcccgg agaagtgttc cgtacccggt aataatttcc attgattcca ggccagatcg
    54901 ctccacgatg cctgaaaacg ggtcttgtct gccgcctgaa gcgggatatc cagcgccaga
    54961 gtgttaatct tgccgctcgg ttgtgtggag cgccaaacat cacccagtgc aggtgaaagt
    55021 ttcgcggcca gcgggcgtat gccctccagg cctgccagct ccagattact ggcgcgaatc
    55081 cggagttcgt cactgcgttt attgtctttg ccgccaacgt cctgttccgg tatccaggcc
    55141 agcgtcaatg ctccgctcgg ccagggtttg ccgtccatcg tgatccgtgt atcgggaata
    55201 gagaactgcc agcctggatt ttcacgcgta atatgcgcgg tcagattatc caccgacagc
    55261 gtatgcgttt gcttctcgcc caaccagctg gcaccgccct gtttcagcca gacgtcaccg
    55321 ccggttacat cgcctttgtc gatcgtcatc cagccttcaa gggagaactg tgccgtttcc
    55381 agcgcaatat tgtcctgcat ccatttaccg agccacggct tcaggtcgat gtcatccgcc
    55441 tggagccaga cgcgaccatt gcttaacaac ccctcatcat cgcgcaaatc catgcgcacc
    55501 tgcatcacgc cgtgctgtcc ggtaaggctg gagaggctta ccaggccttc tgcacggtgt
    55561 cgacgtggat cgttcagcca ggtgagttgt gggatcgcca gctcggcgcg ctgaccggat
    55621 ggcgtcagga aactgacttc actgtcgcgg agatcaaaat ggtcgaactg acgaagaaac
    55681 agatcgctga tgtgactggc ttccaggcta tcattaccac cgccgctggt gataggagtg
    55741 ttggtgcgaa agcgcagctg ccagaaagtg aggtcgcgaa actgccagcg catatgtaac
    55801 aggctctgcc agacatctag cgcaagagta acgcgtttaa ccgaaaattc gccgccatcc
    55861 tttagttctg cacggatgtc gtgtgcttca agcgtcgggc caaaattctg ccagctggct
    55921 gagagctgac tggcttctac cggcatgcca gtcgcggatt ctattttgtt gaggatttcc
    55981 ggacgccagg catcaagatg cggtaaagca atacgcaggc cgctaaccag cagggcagcg
    56041 atcacaacga gcgcggctcc agtaagcagt aatatccccg gcaatcgcct cacccgtcac
    56101 tccttgtctg ctaaaaatgt gactcaaaaa ccctttgccg gatggcggcc cagcatctgt
    56161 ttacatcatt acgacgtcaa actgctcctg gttatagagc ggttcaattt gtactttaac
    56221 ctgtttgcca acgaaaattt ccacttccgc cagcgagtgt gactcttcac ctttcaaggc
    56281 ttcagctact gccggagaag catagaccag gaaacggtcg gaatcataag catggtgtac
    56341 acgaacaatc tcgcgcatga tttcatagca taccgtttcc acggttttca ccgttccgcg
    56401 accgtggcag gttgggcatt cgttacatag tacgtgctca atgctttcgc gggtgcgttt
    56461 acgcgtcatc tccaccagcc ccagcgccga aaaaccatta acgctggttt tcacccggtc
    56521 tttgctcaac gcctgctcca gcgaatgcaa cacgcggcga cggtgatctt cattattcat
    56581 gtcgatgaaa tcaataatga taatcccgcc cagattgcgc aaccgtaact ggcgagcgat
    56641 agcctgcgtc gcttcaatat tggtattgaa aatggtgtcg tccagattgc gatgaccgac
    56701 aaatgctccg gtattgatgt ccacggtggt catcgcttcg gtctggtcga taatcagata
    56761 gccaccggat ttcagttcta ctttgcgttc cagcgcccgc tggatttcgt tttcgacatc
    56821 aaagagatcg aaaatcggct ggcgtccggt gtaatgctcc agcttgctgg tcatctcggg
    56881 aatgtactcc gaggtaaact ccagcaatgc ttcgtaagtc aggcgtgagt caacgcgaat
    56941 gcggtcgagt tcggcgtcgg cgaaatcacg cagaacacgc tgcgccagcg ccagttcgcc
    57001 atacagctga taacgggtct gcgggcgttt tttacgctcc ataactttgg tccagacgcg
    57061 tttcagataa gcggcatcgg aggccagttc agcctcgcca accccttcag ctgcggtacg
    57121 gatgataaac ccgccctgct cgtcgcaata ctctgcgacc acttttttca ggcgttcacg
    57181 ttctgattcg ctttcaatac gttgggaaac cccaacgtga gaagcccctg gcataaacac
    57241 cagatagcga gaaggcagcg tgatatcggt agtcaggcgc gcacctttag tgccaagcgg
    57301 atctttcacc acctgcacca tcagatcttg cccctgacgg accagttccg agatgtcgcg
    57361 taccgtgaat tgcttttgtt cttcacccgc cacacattca gtgtgcggca tgatatcgga
    57421 tgcatgaaga aacgcggctt tatccagccc aatatctaca aaagccgcct gcatacccgg
    57481 aagtacacga cttacacgac ccttgtagat attgcctact attccgcgtc gcgcctcacg
    57541 ttcaatatga atttcctgca gaataccgcc atcaatatac gccactcgcg tttccgaagg
    57601 cgttacgttt actaacaatt cagccgtcat gtttatccct tttctcacgc agtgcgttaa
    57661 aattacttaa taattcatac gtttcaacca gcggtaagcc gactacggcg tgatagctgc
    57721 catttatctt cctgacaaaa cagccaccca gcccctgaat accgtatgca cctgctttat
    57781 ctaacggttc accgctggcg acatagcccg cgatgtcttc gtctgttaac gttctgaaag
    57841 tcacatcggt gaccaccagg caatcgagaa tgtgctggct gtcggccaac gccactgccg
    57901 tcattacctg atgggtctgg cccgataatt tgcgcaacat ctgcaccgca tgctctgcgt
    57961 cgcgcggttt ctccagcact tctccgttca ggataacgat agtatccgca cccagcaccg
    58021 ggagatcctg cgccgtttgc gcgacacccg cctgtgcttt ctcgcgcgcc agacgcacaa
    58081 catactgctg cgcgctctcc tgcggctgac gctgctcctc aatgcccgta acaatacgtt
    58141 caaaggttac gccaagttgc gcaagtaact cctgacgacg cggagaaccg gaagctaaat
    58201 acagagaagt catagaaacc tttattgcac tgcaaactgc tgacggactt tacgcatcag
    58261 caagaaaatc cacggccaga gcactccatt gactacacta ctccagaaca cttccggtct
    58321 gaaagagacg ttaatcacta aaaactctgc ccagaaaaca ataatatcca ccaccagcga
    58381 aagcaacatg acgaccagcg cctgctgcca taatgcgagg ttgcggaaaa gctgatattt
    58441 cagcgccacc aggtaagcaa tgatgctcat cgccaatacg cgtacgccaa gtgtcgagcc
    58501 gctgatcaga tccagtatgg cacccatcac aaaacctgtg cccacattta cgcgatgagg
    58561 caaggccagg atccaataca acaagatgag taagacccag tttggccgga aaacaatcag
    58621 gttatccggc cagggcatga tttgcagcaa cagcgcaatg aggaaagaga gccagattac
    58681 ccagcgtccc tggctacgat agctcgccac tattgccctc ccggcgcacg cgcaggcggt
    58741 tgagcaccac tttgcggcgg cgtagccctt tgtggagagc gattagcagc gggctgtgtc
    58801 ggcgcagcag gcgcagtagc tgcatttcct gtcgccggtt gctgcggagt cggctgagcg
    58861 atccccgttg ccggttcagg taactttggc cccatcgcgt ctggcgaagg caatacctgc
    58921 ggcatcatct gcatcagacg ttcattagca acacgatgca cctcttccgg cgtcatcggg
    58981 ttagcgccgt tacgatccgc gccccacagc agcagcagat aacgcaaacg ttgcagccct
    59041 gcagtcggac gcgcctgaat cacagtataa gcgcgctggg tatcgagttt tacggaagag
    59101 acaaccgcga ccggatagcc ttccgggaaa cgaccgccca gaccggaagt caccagcaca
    59161 tcaccaacac gaatatccgt attcgctggc agatgctcaa gctgcaaatc atccgtacaa
    59221 ccgttaccgg ctgcaattac gcggatatcg ttgcgcagca cctggattgg cagcgcgtgg
    59281 gtcgcatcac aaatcagcag cacgcgactg gtcagtttag cgacggccac cacctgacca
    59341 acaacacctt tgtcgctgat gaccggctgg ccttcataaa cgccattaac gctaccttta
    59401 tcgataacaa cttgatcgct ataaggatcg ttaaccgtgg agataacctg agtcaccatt
    59461 ttctgctcat cctgacgcag cggggaaccc agcagttcgc gcagacgcgc gttctcctgt
    59521 ttgtattgtc caagcatcag cagttcactg tttttcagca acagttcctg acgtaacgcc
    59581 cggttttcaa gttctagttg gtcacgcgag gccagcgtct gcgatacgcc atccagcaat
    59641 tcacgaggag cattggaaac aaagtagaaa ggactgacgg cggtatccat ataagtacgg
    59701 atttgactga acgtccccag gcggctgtcg gcaataataa tgccgagcgc caccagcacc
    59761 gccagaataa ggcgaatctg tagcgacggg ccacggctaa aaattggctt cataagttat
    59821 gcgtattctc gtatcagacc aggcagggta aacagacgct tcccctgcct gcatccgatt
    59881 actcttcgct gaacaggtcg ccgccgtgca tgtcgatcat ttccagcgct ttgccgccac
    59941 cgcgcgccac acaggtcagc gggtcttcag caacaacgac tggaatgccg gtttcttcca
    60001 ttaacaaacg gtcaaggtta cgcagcagtg cgccaccacc ggtgagcacc atgccgcgct
    60061 cggagatgtc ggaagccagt tccggcgggc actgttccag tgcaaccatt accgcgctca
    60121 caataccggt cagcggttcc tgcagtgctt cgaggatttc gttggagttc agggtaaaac
    60181 cgcgtggaac accttctgcc aggttacggc cacgaacttc gatttcacgg acttcatcgc
    60241 ccgggtaagc cgaaccgatt tcgtgcttga tacgttctgc ggtggcttca ccgatcagag
    60301 aaccgtaatt acgacgcaca tagttgatga tagcttcgtc gaaacggtca ccaccaatgc
    60361 gcacggaaga ggagtaaacc acgccgttca aggagataac agcaacttca gtggtaccac
    60421 caccgatatc aaccaccata gaaccggtcg cttcagaaac cggcaggcca gcaccaattg
    60481 cggcagccat cggttcttca atcaggaaga cttcacgggc accagcgccc tgcgcggatt
    60541 cacgaattgc gcggcgttca acctgggtcg cgccaaccgg cacacaaacc agaacgcgcg
    60601 ggcttggacg cataaagctg ttgctgtgca cttgtttgat gaagtgctgg agcatttttt
    60661 cagtcacgaa gaagtcggcg ataacgccgt ctttcattgg gcgaatagca gcaatattgc
    60721 ccggcgtacg gcccagcatc tgcttcgcgt catgacctac tgcagctacg cttttcggtg
    60781 aaccggcacg atcctgacga atggccacca cggaaggctc attcaatacg atgccttgtc
    60841 cttttacata aatgagggta ttcgcagtac ccaggtcaat ggacaagtca ttggaaaaca
    60901 tgccacgaaa ttttttcaac atactaaggg ataatcctga aagctggggc ggaaaagaaa
    60961 atccgcttac tttaccaacc acacgcagca gcgacaaggc gcaaaaatca tctgctacgg
    61021 tgaaaattag tgcagttcgt ttcctttgtt acaaatctct gcctgagtcc agaaaggctt
    61081 aatgcatcag cagcattcct cgcctgtttg caaccgcgta aggtcattca tctgcatatg
    61141 tgctgcaaca atctggcgag cagacaagca cactcccatg agacgcagcg cgcgttattc
    61201 tacgtgaaaa cggattaaac ggcaggttaa accgagtatc tttgtgaata ttttttcacg
    61261 ttagtatcaa gtggctgtga ggacgcgaaa aaatcccctt gcccacctgt aacaccgcgc
    61321 tgaatcaggg tctgccactc gcttcgcgaa cgcacgccgg tggcgtaaac ctgggtgctg
    61381 gtcccggagc aggcttccac caggctttga accagcagct ggttctccgt tcgcttctca
    61441 atgtttctga ccagccccgg atggagcttg agtaactcaa cattaagttc tttgatccaa
    61501 ctggtgctaa ccagcgtcaa accagcctgg ttgacggcta cccgtacccc taaagcattc
    61561 actaaacgaa taacaggttg taaacgactg atatgttgac ctacatcggc ctctgcaagt
    61621 tcaataatta tgcgttttcg ttgtgatttt tcacattgca ttaacgtatc gcgcagccaa
    61681 cgctgaaaac gcgggcgaat cagcgactca acggtaacct gaatcgccag attttcctcc
    61741 ggccagtaac gtaatagtgg aataagacgg ctgatttgca gacggtcata ctcttccgat
    61801 aagccaaact gcaagaccat cggcatatac tccgccgagc taacctcttc attaccatcg
    61861 aagatgcggc acatgagttc gcgatgatga acctgacctt cgcgagtaac cgccggtttt
    61921 tgataaaggc gcgggccgcc gcgactcagc atttgctcga taagcgtacg ccagcgaaca
    61981 ttaccgcgtc ctttttcagg caacgagtca tcgtaaatag cccagctatt gccgccctgc
    62041 aatcccgcat tacgcgtggc agactctgca tgttccatta cctgctcggt atcctgacca
    62101 ctacgccagg cacagatacc aatgtggatc atatcgtcgc gatcgagcat tttattattc
    62161 ggcaaggtat cgacggcttt gattaactga ctggctatgc tctcagcctc ttttaacgtc
    62221 cggtgcggca acagcgcagc aaaatcactg cggtggtaac gcgccagcag tgcgccaggg
    62281 tagcgcatca taaatgtcga cagcagattc gtcagagaga agaactgttc ttcaacctgg
    62341 ctgtgccccc aggtatcact caacatattg aaatccggca gacgaatcat catcacgatc
    62401 ccgtgggtac ctactttctc ctgatcttcc agtaacgttg ctaactgatt atcgaagaag
    62461 agtcggttat tgaggccggt tttcatgtcc tgggcggcat aagagcggat cagcgtatca
    62521 agacggctgt gttgttcacg tgcgttctga atttcacgaa gcagcgtatc cagcgcactg
    62581 ctggttctgg gcggccattc atagatggtt cccaacacat tagagccacg ctcaccgttt
    62641 aagatacgag tagcccgggt ttccagcaat tcttgcccgg caagttgccg ttgtaaccag
    62701 cgtaccgcca ggaagagcat aagaatgata aagccaatcg cccccgtgag cggcgcggtg
    62761 gtcatcaacg aatggaaata gttgcccatc ggatcctgat aaaccagacg cagcgacatc
    62821 cccggatgct ttatcaacgg aacgctcagt tcgcgaaaca gatcgctggt gccaaccgga
    62881 cgataactac catttctggc gagggtataa acctgtttat caccatgcag caggtctaca
    62941 cgaacgatat ctgccgacat cattaattcg gtaatttgtg gccttaatgc gctgaagtca
    63001 ttcgacacaa gatgggtatc aatcgccgtc gccaccgcct gaacgcgatg actaaactta
    63061 tactgaatgg cgttgtagaa acttagcgaa cagcccagca aagtcacaaa aattgttaac
    63121 ccggtgagca gcgtaacaaa ggccgaaaat ttcgtcgtta atctcatcct tgtgttaact
    63181 ccgatagtga ggaagcgggc atactagcaa atcagattta tctcgcaatt tattgcgctt
    63241 catcggcttt gcttttccat tagcgagtat agtcttcaga aattattttc caatccatca
    63301 tgcacatgag gaccacttat gcaggcgtta cttttagaac agcaggacgg caaaactctc
    63361 gcatcggtac agactctgga cgaaagttgc ctgccggagg gcgatgtcac ggttgatgtt
    63421 cactggtcga gcctgaacta caaagatgcg ctggcgatta ccggtaaggg aaaaatcatc
    63481 cgtaattttc cgatgattcc tgggatcgat tttgccggaa ctgtacgcac cagcgaagat
    63541 ccgcgttttc atgccggtca ggaggtgtta ctcactggct ggggcgttgg tgaaaaccac
    63601 tggggggggc tggcggaaca ggcgcgagtg aaaggtgact ggctggttgc catgccgcaa
    63661 gggctggacg cgcgtaaagc aatgattatc ggtactgccg gttttaccgc catgctgtgt
    63721 gtgatggcgc tggaagatgc cggtgttcgc ccgcaggatg gggagattgt cgtgacgggt
    63781 gccagtggtg gcgtcggcag taccgccgtg gcgctgctgc ataagttggg ttatcaggtc
    63841 gttgccgttt ctggtcgcga aagcacccat gaatatctga aaagtttagg agccagccgt
    63901 attctccctc gcgatgagtt tgccgaatcc cgtcctctgg aaaaacaagt ctgggctggg
    63961 gcaattgaca ccgttggcga caaagtgctg gcaaaagtgc tggcgcaaat gaattacggc
    64021 ggctgcgtgg cggcctgtgg tctggcgggt ggttttactc tgccaaccac ggtcatgcca
    64081 tttattctgc gtaatgtccg tttacagggg gtggattcag tgatgactcc accagaacgc
    64141 cgcgcacaag cctggcagcg actggtcgcc gatttaccgg aatcattcta tacccaggcg
    64201 gcaaaagaga tatctctggc agaggcaccg aaatttgccg gggccatcat taataaccag
    64261 atccagggtc gcacgctggt gaaggttaac taaccattta gcagggaata ataagagagg
    64321 gaactcattt tgaaatcatt attcaatcgg ttaacgggaa aagcggttag ccggacagct
    64381 ttcgtcgaac atcttggtca tgaagttgta caacatcatc caaactggaa agtcatgatt
    64441 tcgactgacc ataaattgat gcgcactgtt actccactaa acagccatta ttgatacgcc
    64501 tccgtcgcct gttaggttta tggtgctttg cctgggcgac gctacgctta acctgttact
    64561 gatttctggt accatggggt gaataatctg attttatttg cctacaaatt aaccactcga
    64621 acgtatttga tgctgagcat tgtcaggcgg ttgattttgc gtgctttagc attcacgttt
    64681 atccagacga tgcagtgaaa attgggcaat ccccagcaac cgctgcgtaa tgtcgtctat
    64741 cttgtcgcga tcctggcatc cctacattat ttgtggtctg tgaagattat ctcattgcag
    64801 ccctcatctt cgcagggctg gctttacagc ttttagcctt acgttataag aagttccgtc
    64861 gatgatggcg ctaatttcgt gaattgtgcg gcttgttgca aattacacgg tgttgaaggt
    64921 tatttacatg ttagctgttg attatcttcc ctgataagac cagtatttag ctgccaattg
    64981 ctacgaaatc gttataatgt gcgacctcgt cctccctgac gcagtttttg cgctgcggaa
    65041 aaggtgacat tggcgcaacg aaggtatatt ttgttttttg ccggaggata gcagcagatc
    65101 gctgcacaat gtccgtcaag tctaacattg acactctggg gcaaaataga ccggcgtccc
    65161 ggcctgctgg aatttatcgc tatgcataca gctgtcgggg catacgcttt acagacggcg
    65221 gtgaaacgcc tgtcacaatc acactaaaca aagagtacgg aacccactca tggatattcg
    65281 taagattaaa aaactgatcg agctggttga agaatcaggc atctccgaac tggaaatttc
    65341 tgaaggcgaa gagtcagtac gcattagccg tgcagctcct gccgcaagtt tccctgtgat
    65401 gcaacaagct tacgctgcac caatgatgca gcagccagct caatctaacg cagccgctcc
    65461 ggcgaccgtt ccttccatgg aagcgccagc agcagcggaa atcagtggtc acatcgtacg
    65521 ttccccgatg gttggtactt tctaccgcac cccaagcccg gacgcgaaag cgttcatcga
    65581 agtgggtcag aaagtcaacg tgggcgatac cctgtgcatc gttgaagcca tgaaaatgat
    65641 gaaccagatc gaagcggaca aatccggtac cgtgaaagca attctggtcg aaagtggaca
    65701 accggtagaa tttgacgagc cgctggtcgt catcgagtaa cgaggcgaac atgctggata
    65761 aaattgttat tgccaaccgc ggcgagattg cattgcgtat tcttcgtgcc tgtaaagaat
    65821 tgggcatcaa gactgtcgct gtgcactcca gcgcggatcg cgatttaaaa cacgtattac
    65881 tggcagatga aacggtctgt atcggccctg ctccgtcagt aaaaagttat ctgaacatcc
    65941 cggcaatcat cagcgccgct gaaatcaccg gcgcagtagc aatccatccg ggttacggct
    66001 tcctctccga gaacgccaac tttgccgagc aggttgagcg ctccggcttt atcttcatcg
    66061 gcccgaaagc agaaaccatt cgcctgatgg gcgacaaagt atccgcaatc gcggcgatga
    66121 aaaaagcggg cgttccttgc gtaccgggtt ctgacggccc gctgggcgac gatatggata
    66181 aaaaccgcgc tattgctaaa cgcattggtt atccggtgat tatcaaagcc tccggcggcg
    66241 gcggtggtcg cggtatgcgc gtagtgcgcg gcgacgctga actggcacaa tccatctcca
    66301 tgacccgtgc ggaagcgaaa gctgctttca gcaacgatat ggtttacatg gagaaatacc
    66361 tggaaaatcc tcgccacgtc gagattcagg tactggctga cggtcagggc aacgctatct
    66421 atctggcaga acgtgactgc tccatgcagc gccgccacca gaaagtggtc gaagaagcac
    66481 cggcaccggg cattaccccg gaactgcgtc gctacatcgg cgaacgttgt gctaaagcgt
    66541 gtgttgatat cggctatcgc ggcgcaggta ctttcgagtt cctgttcgaa aacggcgagt
    66601 tctatttcat cgaaatgaac acccgtattc aggtagaaca tccggttacc gaaatgatca
    66661 ccggcgttga cctgatcaaa gaacagctgc gtatcgctgc cggtcaaccg ctgtcgatca
    66721 agcaagaaga agttcacgtt cgcggccatg cggtggaatg ccgtatcaac gccgaagatc
    66781 cgaacacctt cctgccaagt ccgggcaaaa tcacccgttt ccacgcacca ggcggttttg
    66841 gcgtgcgttg ggagtctcat atctacgcgg gctacaccgt accgccgtac tatgactcaa
    66901 tgatcggtaa gctgatttgc tacggtgaaa accgtgacgt ggcgattgcc cgcatgaaga
    66961 atgcgctgca ggagctgatc atcgacggta tcaaaaccaa cgttgatctg cagatccgca
    67021 tcatgaatga cgagaacttc cagcatggtg gcactaatat ccactatctg gagaaaaaac
    67081 tcggtcttca ggaaaaataa gactgcaaaa gcgtcaaaag gccggatttt ccggcctttt
    67141 ttattactgg ggatcgacaa cccccataag gtacaatccc cgctttcttc acccatcagg
    67201 gacaaaaaat ggacactcgt tttgttcagg cccataaaga ggcgcgctgg gcgctggggc
    67261 tgaccctttt gtatctggcg gtatggttag tagccgctta cttacctggc gttgcccccg
    67321 gttttaccgg ctttccgcgc tggtttgaga tggcctgcat cctgacgccg ctgctgttta
    67381 ttggactgtg ctgggcgatg gtgaaattta tctatcgcga tatcccactg gaggatgacg
    67441 atgcagcttg aagtgattct accgctggtc gcctatctgg tggtggtgtt cggtatctct
    67501 gtttatgcga tgcgtaaacg gagcacaggc accttcctta atgagtattt cctcggcagc
    67561 cgctctatgg gcggtattgt gctggcgatg acgctcaccg cgacctatat cagtgccagt
    67621 tcgtttatcg gcgggcctgg cgctgcttat aaatacggcc tgggttgggt attgctggcg
    67681 atgattcagc ttcctgcggt ctggctttcg ctcggtattc tcggcaagaa gttcgcgatt
    67741 cttgcgcgcc gctacaatgc ggtgacgctg aacgatatgc tatttgcccg ctaccagagt
    67801 cgtctgctgg tgtggctggc gagtttaagt ttgctggttg cgttcgttgg tgcgatgacc
    67861 gtgcagttta tcggcggtgc gcgtctgctg gaaaccgcgg cgggtattcc ttatgaaaca
    67921 ggtttgctga tttttggcat cagcattgcg ctctacaccg ccttcggcgg ctttcgcgcc
    67981 agcgtgctga acgacaccat gcaagggctg gtgatgctga ttggcaccat tgtgctgctt
    68041 attggcgtgg tccatgctgc tggcggctta agcaatgcgg tagagacgct gcaaaccatc
    68101 gatccgcaac tggttacgcc acaaggcgct gacgatattc tgtcgcctgc gtttatgacg
    68161 tcgttctggg tgctggtgtg ttttggcgtg attggcctgc cacatactgc ggtgcgctgt
    68221 atctcttata aagacagcaa agccgtacat cgggggatca tcatcggtac gattgtggtc
    68281 gcaatcctga tgttcggtat gcacctggcc ggagcgttag gtcgggcggt gatccccgat
    68341 ctcaccgtac cggacctggt gatcccgacg ttaatggtaa aagtgttgcc accgtttgct
    68401 gccgggatct tcctggctgc acctatggct gcgatcatgt cgacgatcaa tgcccagttg
    68461 ctgcaaagtt ccgctacgat cattaaagat ctctatttga atatccgtcc ggatcaaatg
    68521 caaaacgaga cgcgtctgaa gcggatgtcg gcggtaatta cgttagttct cggtgcgttg
    68581 ctgctgcttg ccgcctggaa gccgccagaa atgatcatct ggctgaattt gctggccttc
    68641 ggtgggctgg aagccgtttt cctgtggccg ctggtactgg gtctttactg ggaacgcgcc
    68701 aacgccaaag gcgcgctaag tgcgatgatt gttggcggcg tactgtatgc cgtactcgcg
    68761 acgctgaata ttcagtacct gggcttccac cctatcgtgc cctcgttact actaagtttg
    68821 ctggctttcc tggtcggaaa ccgtttcggt acatccgtcc cgcaagctac cgttttgact
    68881 actgataaat aaagagtttt gccatgcctt ggatccaact gaaactgaac actaccggcg
    68941 cgaacgcgga agatcttagc gatgcgctga tggaagcggg tgccgtttct atcacttttc
    69001 aggataccca cgatacgcct gtatttgagc cgctgccggg cgaaacgcgc ctgtggggcg
    69061 acaccgatgt gattggtctg ttcgacgctg aaaccgatat gaacgacgtg gtggcgattc
    69121 tggaaaacca tccgctgctc ggcgcgggct tcgcgcataa aatcgaacaa ctggaagata
    69181 aagactggga gcgcgaatgg atggataatt tccacccgat gcgctttggt gaacgactgt
    69241 ggatctgccc aagctggcgt gatgtgccgg acgaaaacgc cgtcaacgtg atgttagacc
    69301 cagggctggc gtttggtacg ggtacccatc caactacctc tctgtgcctg caatggctcg
    69361 acagcctcga tttaaccggt aaaacagtca tcgactttgg ctgcggttcc ggcattctgg
    69421 ctatcgcggc gctgaaactg ggtgcagcaa aagccattgg tattgatatt gatccgcagg
    69481 cgattcaggc cagccgcgac aacgcggaac gtaatggcgt ctctgaccgt ctggaactct
    69541 acttaccgaa agatcagcca gaagaaatga aagccgacgt ggtggtcgct aacatccttg
    69601 caggcccatt acgtgaactg gcaccgttaa tcagcgtcct gccagtttca ggcggtttgc
    69661 tgggcctttc cggtattctg gcaagccagg cagagagcgt ttgcgaagct tatgccgaca
    69721 gcttcgctct ggacccggtc gtggaaaaag aagagtggtg ccgcattacc ggtcgtaaga
    69781 attaaccttc gcatcgccgt agggtgacgc agggcgaagt gcgagcaagg tcacaaaagg
    69841 cacgtgaatt tgccgattat ttacgcaaat ttgcgtgcca aaattttcat tcataaagaa
    69901 aaagtgagaa cttactcaaa tttctttgag tgtaaatttt agtcactatt ttctaatatg
    69961 atgattttta tgagtaatta tcgcgctacg ctcattttaa atgcaattct ttgatccatc
    70021 tcagaggatt ggtcaaagtt tggcctttca tctcgtgcaa aaaatgcgta atatacgccg
    70081 ccttgcagtc acagtatggt catttcttaa ctcatgcgca tcggacaata tcagctcaga
    70141 aatcgcctga tcgcagcgcc catggctggc attacagaca gaccttttcg gacgttgtgc
    70201 tacgagatgg gagccggatt gacagtatcc gagatgatgt cttctaaccc tcaggtttgg
    70261 gaaagcgaca aatctcgttt acggatggtg cacattgatg aacccggtat tcgcaccgtg
    70321 caaattgctg gtagcgatcc gaaagaaatg gcagatgcag cacgtattaa cgtggaaagc
    70381 ggtgcccaga ttattgatat caatatgggt tgcccggcta aaaaagtgaa tcgcaagctc
    70441 gcaggttcag ccctcttgca gtacccggat gtcgttaaat cgatccttac cgaggtcgtc
    70501 aatgcagtgg acgttcctgt taccctgaaa attcgcactg gctgggcacc ggaacaccgt
    70561 aactgcgaag agattgccca actggctgaa gactgtggca ttcaggctct gaccattcat
    70621 ggccgtacac gcgcctgttt gttcaatgga gaagctgagt acgacagtat tcgggcagtt
    70681 aagcagaaag tttccattcc ggttatcgcg aatggcgaca ttactgaccc gcttaaagcc
    70741 agagctgtgc tcgactatac aggggcggat gccctgatga taggccgcgc agctcaggga
    70801 agaccctgga tctttcggga aatccagcat tatctggaca ctggggagtt gctgcccccg
    70861 ctgcctttgg cagaggttaa gcgcttgctt tgcgcgcacg ttcgggaact gcatgacttt
    70921 tatggtccgg caaaagggta ccgaattgca cgtaaacacg tttcctggta tctccaggaa
    70981 cacgctccaa atgaccagtt tcggcgcaca ttcaacgcca ttgaggatgc cagcgaacag
    71041 ctggaggcgt tggaggcata cttcgaaaat tttgcgtaaa cagaaataaa gagctgacag
    71101 aactatgttc gaacaacgcg taaattctga cgtactgacc gtttctaccg ttaactctca
    71161 ggatcaggta acccaaaaac ccctgcgtga ctcggttaaa caggcactga agaactattt
    71221 tgctcaactg aacggtcagg atgtgaatga cctctatgag ctggtactgg ctgaagtaga
    71281 acagcccctg ttggacatgg tgatgcaata cacccgtggt aaccagaccc gtgctgcgct
    71341 gatgatgggc atcaaccgtg gtacgctgcg taaaaaattg aaaaaatacg gcatgaacta
    71401 attcaggtta gctaaatgct tgattaaaaa ggcgctactc ggcatgggga agcgcctttt
    71461 ttataggtgt cacaaaggga gtgaccatga gaacaggatg tgaaccgacc cgatttggta
    71521 atgaagcaaa gaccatcatc cagggtgatg cattaaccga acttaaaaag ctccccgccg
    71581 aaagtgtcga tctgatcttt gccgacccac cgtataacat cggtaaaaat tttgatggtc
    71641 tgatcgaagc ctggaaagaa gatctgttta tcgactggct gtttgaagtg attgcagagt
    71701 gccatcgcgt tctgaaaaag cagggcagca tgtacatcat gaatagcacg gaaaacatgc
    71761 cctttatcga tctccagtgc cgcaagcttt ttaccatcaa aagtcgcatc gtctggtcat
    71821 atgacagttc tggagtgcag gcgaaaaaac actacggctc catgtacgaa cccatcctga
    71881 tgatggtgaa agacgcaaag aactacacat tcaacggtga tgctattctg gtagaagcca
    71941 aaaccggatc gcagcgcgcg ttgatcgatt atcgcaaaaa ccctccacag ccttacaatc
    72001 atcaaaaagt accgggtaac gtctgggatt ttccgcgcgt gcgttatttg atggatgaat
    72061 atgaaaacca cccgacgcaa aaaccggaag ccttactgaa acgcattatt ctcgcctctt
    72121 ccaacccagg cgatatcgtt ctcgacccgt ttgctggtag ctttactacc ggtgccgtag
    72181 ccgtcgccag tgggcgaaaa ttcattggta ttgagatcaa cagcgagtac atcaaaatgg
    72241 ggcttcgacg gctggatgtc gcgtcgcatt actctgcgga agaactggcg aaagtgaaaa
    72301 aaagaaagac cggcaacctg tcaaaacgaa gcaggttaag cgaagttgac cccgatctca
    72361 ttgcaaagta aaggaagtgt aagcctggtt ttcagattat tcatttcgtg tatatttcct
    72421 gccagacttg gttaaacatg cacaggctca ggtaatgatt cgcaagtatt ggtggctcgt
    72481 cgttttcgct gtcttcgttt ttctgtttga tactttactg atgcagtgga ttgaactgct
    72541 cgcaacagaa acagacaaat gccgcaatat gaactcagtt aatccactaa aactggttaa
    72601 ctgtgacgaa ctgaattttc aggacagaat gtgaatttat atgacactta attcattcgt
    72661 ttgatgaatt aatttcgtta tgttttcatc tggcatgaac attcttaata cgttatcgac
    72721 cagtgcgggt gcttgtttat aaagatcata acccgccata ttcattaacc agttttgaac
    72781 aattccgctg aaggcaccat caataataat catcacaaca tctaaatcga ggttatttgc
    72841 tacacaacct tgttgctgac acgcctgcaa tacttcgcgg agattctgcg gattaaagcc
    72901 catcttttcg cgtatcactc cctcggccag catctcatca ttaaattcac atttgtgata
    72961 taagattttc agcaacgcct gctggcgggg aattttggca atatattgca agccgacaat
    73021 caatttttca cgcaattgtt gaaacgggtc atgctctaat ccagccgtca agtgttcctg
    73081 gattaactcc cacaatgaag gctgttgcaa ccacatctca ttaaacagtt gagtcttgtt
    73141 ttcgaagtgc cagtagatag cgccacgcgt aacgttagcg gcgtcggcaa tgtcgttgag
    73201 cgtcgtcttg cttacgccat gctgcgcaaa ctgggcgatg gcagtttcaa tcagttcttg
    73261 ccgggtcttc agggcttcag ctttggttct ttttgccatg attaattatt caggaaataa
    73321 atatattcga cacaggatga gaaaatagcg aaggttaatc tatcacctaa tgtatattta
    73381 tacgagaggc taatattgag ttgctataaa tcgtcaaata aataacgttt attatttacc
    73441 taaaatacat tcactctatc aatatatatt tcaatttacg aggatttgat tctacctctt
    73501 tcaacccgcg tcaaaataaa acagtagaat attaatcttt ttttgtgttt atgtgccttg
    73561 agacgccagt attcataact attccttaca tcgacgaatg ataatttgta ggatagcgaa
    73621 ctgtattttt ctttctgcga gttaacgcgt tgcctttttg ggtaaataac gcgcttttgg
    73681 ttttttgagg aatagtaatg acgaaacatg ccaggttttt cctcctgccc tcctttattc
    73741 tgatctccgc ggctttaatc gccggttgta acgataaggg agaagagaaa gctcacgtcg
    73801 gtgaaccgca ggttaccgtt catattgtga aaacggcccc gttagaagtt aagactgaat
    73861 taccaggccg caccaatgct taccgtatag ccgaagttcg cccacaggtt agcgggatcg
    73921 tactgagtcg caatttcact gaaggcagcg atgtgcaagc aggccagtct ctgtaccaga
    73981 tcgatcccgc gacctatcag gcaagttatg acagcgcgaa aggcgaactg gcgaaaagtg
    74041 aagccgccgc cgccatcgcg catttgacgg taaaacgtta cgttccgctc gtgggtacga
    74101 aatacatcag ccagcaggag tacgaccagg ccattgctga tgctcgccag gccgatgccg
    74161 ccgtgattgc cgcaaaagcc acagtcgaaa gcgctcacat caatcttgct tataccaaag
    74221 tgactgcgcc aattagcgga cgtatcggca aatcgactgt gaccgaaggc gctcttgtca
    74281 ctaatgggca aacgactgaa ctggcgactg tccagcagct cgatcctatc tacgttgatg
    74341 tgacccaatc cagcaacgat tttatgaggc tgaagcaatc cgtagagcaa ggaaatttgc
    74401 ataaggaaaa cgccaccagc aacgtagagt tggtcatgga gaacggtcaa acctatcccc
    74461 tgaaaggtac gctgcaattc tccgatgtta ccgttgatga aagcaccggc tccataaccc
    74521 tacgtgctgt cttccctaac ccgcaacata cgcttttacc tggtatgttt gtgcgcgcac
    74581 ggattgatga aggcgtccaa tctgatgcca ttcttatccc gcaacaaggc gttagccgca
    74641 caccgcgtgg tgatgcaacc gtgctgattg ttaacgataa aagtcaggtt gaagcgcgcc
    74701 ctgtcgttgc cagccaggcg attggcgata aatggttgat tagcgaaggg ctgaaatctg
    74761 gcgatcaagt cattgtcagc ggcctgcaaa aagcgcgtcc gggagagcag gttaaagcca
    74821 ctaccgatac ccccgcagat tctgcatcga agtaaggcaa tctgacatgg caaacttttt
    74881 tattcgacga ccgatatttg catgggtgct ggccattatt ctgatgatgg cgggtgtact
    74941 ggcgatccta caattgcccg tcgcccaata cccaaccatt gcgccgcctg cagtttctgt
    75001 ttcggcaaac tatccgggcg ctgatgcgca gaccgtgcag gatacggtga cgcaggttat
    75061 cgaacagaat atgaatggta tcgataacct gatgtatatg tcctccacca gcgattctgc
    75121 cagtagcgtg acaattaccc ttaccttcca gtccgggacc gatccagata tcgcgcaagt
    75181 gcaggtgcag aacaaactcc agctcgccac gccgttgctg ccgcaggaag tacagcagca
    75241 ggggatcagt gttgaaaagt ccagtagcag ctatttgatg gtggcgggct ttgtctctga
    75301 taacccagac accacacagg acgatatctc ggactatgtg gcctctaacg ttaaagatac
    75361 gcttagccgt ctgaatggcg tcggtgacgt acagcttttc ggcgcacagt atgcgatgcg
    75421 tatctggttg gacgccgatc tgctaaacaa atataaactg acgcctgttg atgtgattaa
    75481 ccagttgaag gtacagaacg atcagatcgc tgccggacag ttgggcggaa cgccagcgtt
    75541 accagggcaa cagttgaacg cctcgattat tgctcagacg cggttaaaaa atccggaaga
    75601 attcggcaaa gtgaccttgc gcgtaaacag tgacggctcg gtggtacgcc tgaaagatgt
    75661 cgcacgggtt gaacttggcg gtgaaaacta taacgttatc gctcgcatca acggtaaacc
    75721 ggcggcgggc ctggggatta agctggcaac cggcgcgaat gctctcgata ccgcgaaagc
    75781 cattaaggca aaactggcgg aattacagcc attcttcccg cagggaatga aggttctcta
    75841 cccttatgac accacgccat tcgtccagct ttctattcac gaagtggtaa aaacgctgtt
    75901 cgaagctatt atgctggtgt tcctggtgat gtatctgttc ttgcagaata tgcgagcaac
    75961 gctgatcccc accattgcgg tacccgtggt gttgttaggg acgtttgcca tcctcgccgc
    76021 ttttggttac tccatcaaca cactaacgat gttcgggatg gtgcttgcca tcgggctgct
    76081 cgtcgatgat gcgatagtgg tggtggagaa cgtcgagcgc gtgatgatgg aggataagct
    76141 cccgccaaaa gaagcgacgg aaaaatcaat gtcgcaaatc cagggcgcac tggtgggtat
    76201 cgcaatggtg ctgtcagcgg tatttattcc gatggcattc ttcggcggtt ctactggggc
    76261 aatttatcgc cagttctcta tcaccatcgt ttcggcaatg gcgctttctg ttctggtggc
    76321 attgattctt accccagcgt tatgtgcaac actgctcaaa cccgtctctg ctgagcatca
    76381 cgaaaataag ggcggtttct tcggctggtt taataccacc ttcgatcata gcgttaacca
    76441 ctacaccaac agcgtcggca aaatcctcgg ttccacagga cgatatttac tgatctatgc
    76501 actaattgtt gctggaatgg tggtgttgtt tttacgtctt ccgtcttcct tcttacctga
    76561 agaggatcag ggtgtctttc tgactatgat tcagttaccc gctggcgcga cgcaagagcg
    76621 aacgcaaaaa gtgttggatc aagtgacgga ttactatctg aagaacgaga aagcgaacgt
    76681 agaaagtgtc tttacggtta acggctttag cttcagcggc caggcacaaa acgccggtat
    76741 ggctttcgtc agtctgaaac cgtgggaaga gcgtagtggt gacgaaaaca gtgcggaagc
    76801 ggtaatccat cgtgccaaaa tggaattggg caagatccgc gacggttttg tcattccatt
    76861 caatatgcca gccattgttg aactgggcac ggcaacgggt ttcgactttg agttaattga
    76921 tcaggctggg ctgggtcatg atgccctgac ccaggcccgt aaccagttgc ttggcatggc
    76981 ggcgcaacat cctgccagct tagtcagcgt gcgtcctaat ggtctggaag acaccgcgca
    77041 gtttaaactg gaagttgacc aggaaaaggc gcaggcatta ggtgtttcac tttctgacat
    77101 caatcagacc atttcaacgg cgttaggtgg gacttacgtt aacgacttca ttgaccgtgg
    77161 tcgcgtgaaa aaggtgtatg ttcaggcgga tgccaaattc cgtatgctgc cagaagatgt
    77221 cgataaactt tatgtccgca gcgccaacgg cgaaatggtg ccattctcgg cctttaccac
    77281 tgcacattgg gtgtatggct ctccgcgact ggagcgctac aacggtctgc cgtcaatgga
    77341 gattcagggg gaagccgcgc caggaaccag ttccggcgat gccatggcgt tgatggaaaa
    77401 ccttgcatca caattaccag ctggcattgg ttatgactgg acgggtatgt catatcagga
    77461 acgcttatcg ggaaaccagg ctcccgctct ggtagcaatt tcctttgtgg ttgttttcct
    77521 gtgccttgct gcactctatg aaagctggtc aattcctgtc tcggttatgt tggtcgtgcc
    77581 gttagggatt gtcggcgtgc tactggcggc gacactcttt aatcaaaaaa atgacgtcta
    77641 ctttatggtg ggcttgctaa cgacaattgg cttgtcggct aaaaacgcta ttttgattgt
    77701 tgagttcgct aaagatctca tggagaaaga gggtaaaggt gttgttgaag cgacactgat
    77761 ggcagtacgt atgcgcctgc gtcctatcct gatgacctct ctcgccttta ttctcggcgt
    77821 attaccgcta gctatcagta acggtgccgg cagtggcgcg cagaacgcag tgggtatcgg
    77881 ggtaatgggc gggatggttt ctgcaacgtt gctggcaatc ttcttcgtgc cggtgttctt
    77941 tgtggtgatc cgccgttgct ttaaaggata aatcagaaac ataaaggcac tttcgggtgc
    78001 ctttattatt ttcagtgaaa cccataaaaa ttaataagat attcttcttc tcactcttta
    78061 aaaactttct atagttcccg ctcccttcac tatttttaca attcacataa tttgtcatta
    78121 ttcattccgc aagatttata acctccggtg ataaaatggc attcagctcg ttaataagag
    78181 agttaactta ttaagcgtta gcgtttatta ctgaggtaac accatgaaaa gattaattcc
    78241 tgtggcattg ctcaccgcat tgctggcggg ctgtgctcac gattcgccct gcgttccggt
    78301 ttatgacgat cagggtcgac tggttcacac taatacctgt atgaaaggca cgactcagga
    78361 taactgggaa acggctgggg ctattgctgg cggggcagca gctgttgctg gcctgacgat
    78421 ggggatcatc gctctgtcga aataactgca caaggcgcgg agtaccgcgc tctgtttaac
    78481 gatacagtat ctctccataa gataaaatat tgctatattc tgctgggtaa ttccctgcta
    78541 atttagcatg ttatatcgcg ttatttcgct gctttcgaaa taacgcatca atttaatttt
    78601 cacacttcct accagaaata tactcacgct aacaatcatt attctgctta gtaaaaaatg
    78661 cgtaaatatt ttgcgccaaa atgtggcgca tgtttcattt tcgcaccatt gcggggcgct
    78721 gtttttatta tttcctgtct acactcagct tattgcgacg cgaaatcgtg cgcaaaagct
    78781 ggcactactt ttgcttataa gaggatggcc acagacaggt aaaagacgtt tcccaaacgt
    78841 cctataacga taattttcgc ctcacaggaa gcattatgaa aaagatgatg atagccacac
    78901 tggctgccgc cagcgtgctg cttgccgttg caaatcaggc gcatgctggc gcgacgcttg
    78961 atgccgttca gaaaaaaggt tttgtgcaat gcgggatcag tgatggatta cctgggttct
    79021 cttatgccga tgctgacggt aagttttcag gtattgatgt agatgtttgt cgtggtgttg
    79081 cggcagctgt atttggtgac gacacgaaag tgaaatatac cccgctcact gcaaaagaac
    79141 gcttcacggc tttacagtca ggggaggtgg atttgctctc ccgtaatacg acctggactt
    79201 catctcgcga tgccgggatg ggaatggcat ttactggcgt cacttattac gacggcattg
    79261 gcttcctgac tcacgataaa gcgggattaa aaagcgcgaa agaactggat ggcgctaccg
    79321 tctgtattca ggcggggact gataccgaac tcaacgttgc cgattacttc aaggcaaaca
    79381 atatgaagta cacaccggtg actttcgatc gctctgacga atcagcgaag gcactggaat
    79441 ctggtcgctg cgatacgctg gcctcggatc aatcacaact gtatgccctg cgcatcaaat
    79501 taagcaaccc agctgaatgg attgtcttac cggaagttat ctcaaaagaa ccgcttggtc
    79561 cggtagttcg tcgtggcgat gatgaatggt tctcgattgt acgctggacg cttttcgcca
    79621 tgctgaatgc tgaagagatg ggcatcaatt cccagaacgt cgatgaaaaa gaggctaatc
    79681 cagcaacgcc tgatatggca catctgttgg gtaaagaagg cgattacggc aaggatctga
    79741 agttggataa taaatgggcc tataacatca tcaaacaggt gggtaactac tcggaaattt
    79801 ttgagcgtaa cgtaggttca gaaagcccgc tgaaaattaa acgtgggcaa aataatctct
    79861 ggaataacgg cggtattcag tacgcgccgc ccgtgcgtta agtcggtgtg aggtaacgag
    79921 cgtcgctacg gcggcgctct cttcagcgtt atgctttccg aggtttctat gtctcatcgc
    79981 cgctcaaccg ttaaaggctc actctccttt gccaacccta cggttcgtgc ttggttattc
    80041 cagatccttg ccgttgttgc tgttgtcggc attgttggtt ggttatttca caacactgta
    80101 acgaatctca ataatcgtgg cattacttca ggttttgcct ttctggatcg cggcgctggc
    80161 ttcggtattg tccagcattt gatcgattac cagcagggcg acacctacgg acgcgttttt
    80221 attgtcggct tactcaatac gctactggtt tctgcattgt gtattgtgtt cgcttctgtt
    80281 ttgggcttct ttatcggtct ggcgagactt tcggataact ggctgctacg aaagctttcc
    80341 acaatttata ttgagatctt ccgtaatatt ccgccgctgc tgcaaatctt cttctggtac
    80401 tttgccgtgt tgcgcaattt gcccggacca cgccaggctg tgagcgcgtt tgatctggcc
    80461 tttttgagca atcgtgggct ttatattccg tcaccgcagc tgggagacgg atttattgcg
    80521 tttatcgtga ctgttgttat tgctatagcc atttctgttg gactattccg ttttaataaa
    80581 acgtaccaga taaagaccgg acaattgcgc cgaacctggc cggtcgccgt tatgttgatc
    80641 ataggtttgc ctttactggc gcaatggctt tttggcgcag cactacactg ggatgttcca
    80701 gccctacgag gctttaactt ccgtggcggg atggttttaa ttcctgaact ggcagcctta
    80761 acgctggcgc tttcggttta tacctctgca tttatcgccg agattatccg cgctgggatc
    80821 caggcagtgc cttatggtca acatgaagcg gctcggtcac tgggattacc caatccggtt
    80881 acgctacgcc aggtcattat tccccaggca ctgcgagtga ttattccgcc gttaaccagc
    80941 cagtatctca acatcgtcaa aaactcctct cttgccgccg cgattggcta tccggatatg
    81001 gtttcgctgt ttgctggcac cgtgctgaac cagacggggc aagccatcga gacgatagcc
    81061 atgaccatgt cggtctatct gattatcagc ctgactatct cgctgctgat gaatatctat
    81121 aaccgccgca tcgcgatcgt tgaacgctaa ggagtcatga tgacaaaagt attgctgtct
    81181 catcccccgc gcccggcgag ccataactca agccgcgcga tggtgtgggt gcgaaaaaat
    81241 ctgttctcca gctggagcaa tagcctgctg actattggct gcatatggtt gatgtgggaa
    81301 ctgattccac cgttgctgaa ctgggcattt ttgcaggcta actgggttgg ctcaacgcgt
    81361 accgactgca caaaagccgg tgcctgttgg gtcttcatcc acgaacgatt tggtcagttt
    81421 atgtatgggc tttacccaca cgaccaacgc tggcgaatta acctcgcatt actgattggg
    81481 cttgtgtcga tcgcaccaat gttctggaaa atactcccgc atcgcggtcg ctatattgcg
    81541 gtatgggcgg tgatttaccc actgattgtc tggtggctga tgtatggcgg gtttcttggt
    81601 cttgagcggg ttgaaacccg gcaatggggc gggctgacgc taactttaat tattgcatca
    81661 gttgggattg cgggggcgct gccgtggggg atcttactgg cgttaggtcg ccgctcccat
    81721 atgccgattg tgcgtatctt atcggtcatt tttatcgagt tctggcgcgg tgtaccgctg
    81781 attaccgttc tgtttatgtc ttcggtcatg ctgccgttgt ttatggcaga aggcaccagt
    81841 atcgacaaat tgatccgcgc gctggttggc gtgatcctgt ttcagtcagc atatgttgcg
    81901 gaagtcgtgc gaggcggatt acaggcgctg cctaaagggc aatatgaagc ggcagagtcg
    81961 ctggcgttgg gttactggaa aacccagggg ctggttattc tgccacaggc gttgaagctg
    82021 gtgattcctg ggctggtaaa taccatcatc gcactcttca aagataccag cctggtgatc
    82081 atcatcgggt tgttcgatct tttcagtagc gttcagcagg caaccgttga tcccgcctgg
    82141 ttgggtatgt cgacggaagg gtatgttttc gccgcactga tctactggat cttctgtttc
    82201 agcatgtcac gttatagcca acatctggaa aaacgtttta acaccgggcg tacaccgcat
    82261 tgaggacact atgagccaaa ttttactgca acctgctaac gcgatgatta cgctggaaaa
    82321 cgtcaataaa tggtatgggc aattccatgt tttgaaaaat ataaatttaa ccgtgcaacc
    82381 gggagaacgg atcgttctgt gtggcccttc tggttccggt aaatcgacaa ccattcgttg
    82441 tattaatcat ctggaagaac atcaacaggg acggatcgtg gtggatggca tcgaacttaa
    82501 tgaagatatc cgcaatattg agcgcgtcag gcaggaagtg ggaatggtct ttcagcattt
    82561 caatctcttc cctcatctga ccgttctaca gaactgtacc ctggcaccga tttgggtacg
    82621 caagatgcct aagaaagagg ctgaagatct ggcgttgcat taccttgagc gggtgagaat
    82681 tgccgaacat gcccagaagt ttcccggaca gatttctggt ggtcagcagc aacgcgttgc
    82741 catagcacgt tcactttgta tgaaaccaaa gatcatgcta tttgatgaac ctacgtcagc
    82801 gctcgatccg gagatggtga aagaagtgct ggatacgatg attgggctgg cgcagtcggg
    82861 tatgacaatg ttgtgtgtaa cacatgagat ggggtttgca cgaaccgtcg ctgacagggt
    82921 gatttttatg gatcgtgggg aaatagtgga gcaagctgca cctgatgaat tttttgcgca
    82981 tcctaaatca gagcgtacga gggcattttt atcgcaggta atccattaat tgaatgttag
    83041 ttcgaaaagc aaaaaggcca tcctttcgga tggcctttcg ct
//
LOCUS       DAOTRK010000005        81660 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.005, whole genome shotgun sequence.
ACCESSION   DAOTRK010000005 DAOTRK010000000
VERSION     DAOTRK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.005"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..203)
                     /gene="rpmE"
                     /locus_tag="QIE41_000353"
     CDS             complement(<1..203)
                     /gene="rpmE"
                     /locus_tag="QIE41_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDS3529286.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPG"
     gene            406..2604
                     /gene="priA"
                     /locus_tag="QIE41_000354"
     CDS             406..2604
                     /gene="priA"
                     /locus_tag="QIE41_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDS3529287.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAMLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTHRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2760..3785
                     /gene="cytR"
                     /locus_tag="QIE41_000355"
     CDS             2760..3785
                     /gene="cytR"
                     /locus_tag="QIE41_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDS3529288.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            3877..4836
                     /gene="ftsN"
                     /locus_tag="QIE41_000356"
     CDS             3877..4836
                     /gene="ftsN"
                     /locus_tag="QIE41_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDS3529289.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            4929..5459
                     /gene="hslV"
                     /locus_tag="QIE41_000357"
     CDS             4929..5459
                     /gene="hslV"
                     /locus_tag="QIE41_000357"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDS3529290.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5469..6800
                     /gene="hslU"
                     /locus_tag="QIE41_000358"
     CDS             5469..6800
                     /gene="hslU"
                     /locus_tag="QIE41_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDS3529291.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            6867..7793
                     /gene="menA"
                     /locus_tag="QIE41_000359"
     CDS             6867..7793
                     /gene="menA"
                     /locus_tag="QIE41_000359"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDS3529292.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            7886..8371
                     /gene="rraA"
                     /locus_tag="QIE41_000360"
     CDS             7886..8371
                     /gene="rraA"
                     /locus_tag="QIE41_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDS3529293.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8456..8701)
                     /gene="zapB"
                     /locus_tag="QIE41_000361"
     CDS             complement(8456..8701)
                     /gene="zapB"
                     /locus_tag="QIE41_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDS3529294.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9126..9971
                     /gene="glpF"
                     /locus_tag="QIE41_000362"
     CDS             9126..9971
                     /gene="glpF"
                     /locus_tag="QIE41_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDS3529295.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            9994..11502
                     /gene="glpK"
                     /locus_tag="QIE41_000363"
     CDS             9994..11502
                     /gene="glpK"
                     /locus_tag="QIE41_000363"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDS3529296.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11637..12647
                     /gene="glpX"
                     /locus_tag="QIE41_000364"
     CDS             11637..12647
                     /gene="glpX"
                     /locus_tag="QIE41_000364"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDS3529297.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12744..13490
                     /gene="fpr"
                     /locus_tag="QIE41_000365"
     CDS             12744..13490
                     /gene="fpr"
                     /locus_tag="QIE41_000365"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDS3529298.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13495..13923)
                     /gene="uspD"
                     /locus_tag="QIE41_000366"
     CDS             complement(13495..13923)
                     /gene="uspD"
                     /locus_tag="QIE41_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDS3529299.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(13950..14249)
                     /locus_tag="QIE41_000367"
     CDS             complement(13950..14249)
                     /locus_tag="QIE41_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDS3529300.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14461..14901)
                     /locus_tag="QIE41_000368"
     CDS             complement(14461..14901)
                     /locus_tag="QIE41_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDS3529301.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTEDVKYKADNKSSN"
     gene            15002..15601
                     /locus_tag="QIE41_000369"
     CDS             15002..15601
                     /locus_tag="QIE41_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDS3529302.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15709..16476
                     /gene="tpiA"
                     /locus_tag="QIE41_000370"
     CDS             15709..16476
                     /gene="tpiA"
                     /locus_tag="QIE41_000370"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDS3529303.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16531..17286)
                     /gene="cdh"
                     /locus_tag="QIE41_000371"
     CDS             complement(16531..17286)
                     /gene="cdh"
                     /locus_tag="QIE41_000371"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDS3529304.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRHDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17393..18382)
                     /gene="sbp"
                     /locus_tag="QIE41_000372"
     CDS             complement(17393..18382)
                     /gene="sbp"
                     /locus_tag="QIE41_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDS3529305.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18702..19664)
                     /gene="pfkA"
                     /locus_tag="QIE41_000373"
     CDS             complement(18702..19664)
                     /gene="pfkA"
                     /locus_tag="QIE41_000373"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDS3529306.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(19845..20747)
                     /gene="fieF"
                     /locus_tag="QIE41_000374"
     CDS             complement(19845..20747)
                     /gene="fieF"
                     /locus_tag="QIE41_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDS3529307.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(20896..21396)
                     /gene="cpxP"
                     /locus_tag="QIE41_000375"
     CDS             complement(20896..21396)
                     /gene="cpxP"
                     /locus_tag="QIE41_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDS3529308.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSVDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21546..22244
                     /gene="cpxR"
                     /locus_tag="QIE41_000376"
     CDS             21546..22244
                     /gene="cpxR"
                     /locus_tag="QIE41_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDS3529309.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22241..23614
                     /gene="cpxA"
                     /locus_tag="QIE41_000377"
     CDS             22241..23614
                     /gene="cpxA"
                     /locus_tag="QIE41_000377"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDS3529310.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23870..24544)
                     /gene="yiiM"
                     /locus_tag="QIE41_000378"
     CDS             complement(23870..24544)
                     /gene="yiiM"
                     /locus_tag="QIE41_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDS3529311.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDEQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24693..25676)
                     /gene="kdgT"
                     /locus_tag="QIE41_000379"
     CDS             complement(24693..25676)
                     /gene="kdgT"
                     /locus_tag="QIE41_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDS3529312.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25936..26556)
                     /gene="sodA"
                     /locus_tag="QIE41_000380"
     CDS             complement(25936..26556)
                     /gene="sodA"
                     /locus_tag="QIE41_000380"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDS3529313.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26841..27875
                     /gene="rhaT"
                     /locus_tag="QIE41_000381"
     CDS             26841..27875
                     /gene="rhaT"
                     /locus_tag="QIE41_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDS3529314.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGID
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(27872..28738)
                     /gene="rhaR"
                     /locus_tag="QIE41_000382"
     CDS             complement(27872..28738)
                     /gene="rhaR"
                     /locus_tag="QIE41_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDS3529315.1"
                     /translation="MAEVATVAHQIKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEALCSERVLRQQ
                     FRQQTGMTINQYLRQIRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSHWRHLNSQKD"
     gene            complement(28794..29630)
                     /gene="rhaS"
                     /locus_tag="QIE41_000383"
     CDS             complement(28794..29630)
                     /gene="rhaS"
                     /locus_tag="QIE41_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDS3529316.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29918..31387
                     /gene="rhaB"
                     /locus_tag="QIE41_000384"
     CDS             29918..31387
                     /gene="rhaB"
                     /locus_tag="QIE41_000384"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDS3529317.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVGSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRNIYQRSGIQFLPFNTLYQLHALTEQQPELIPHIAHALLM
                     PDYFSYRLTGEMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31384..32643
                     /gene="rhaA"
                     /locus_tag="QIE41_000385"
     CDS             31384..32643
                     /gene="rhaA"
                     /locus_tag="QIE41_000385"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDS3529318.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLKSVRAYEKEILSRRG"
     gene            32726..33550
                     /gene="rhaD"
                     /locus_tag="QIE41_000386"
     CDS             32726..33550
                     /gene="rhaD"
                     /locus_tag="QIE41_000386"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDS3529319.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTHEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            33560..33874
                     /gene="rhaM"
                     /locus_tag="QIE41_000387"
     CDS             33560..33874
                     /gene="rhaM"
                     /locus_tag="QIE41_000387"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDS3529320.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(33915..35309)
                     /locus_tag="QIE41_000388"
     CDS             complement(33915..35309)
                     /locus_tag="QIE41_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDS3529321.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKQNDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNLYFELNTLLEDGTSLYNDGNYHGRD
                     VEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            35786..36232
                     /locus_tag="QIE41_000389"
     CDS             35786..36232
                     /locus_tag="QIE41_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDS3529322.1"
                     /translation="MAALTASCIDLNIQGNGTYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            36243..37694
                     /locus_tag="QIE41_000390"
     CDS             36243..37694
                     /locus_tag="QIE41_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDS3529323.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGIENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDHIFSE
                     LPTNSQFFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTQQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELAHEH"
     gene            37684..38754
                     /locus_tag="QIE41_000391"
     CDS             37684..38754
                     /locus_tag="QIE41_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDS3529324.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSILNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVVALKSCATH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKINAFSQFRQVD"
     gene            38754..40502
                     /locus_tag="QIE41_000392"
     CDS             38754..40502
                     /locus_tag="QIE41_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDS3529325.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGNAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERGFCLASRPGLGHFIDETEEKRIILLANLLRKDPFLIPLA
                     GVTRDNLQQLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGSEIKQIV
                     EYSGLFLGDNAVRTLTGLIEKQHQQAQVICVDNVLALLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSDITAISQE
                     ITPLLIVNNSHYLLDDAVKNSITIKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHFSNVRGENWQHITQQICAQLVTQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVVNHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLKGYEAFMELLHKG"
     gene            complement(40558..41199)
                     /locus_tag="QIE41_000393"
                     /pseudo
     CDS             complement(40558..41199)
                     /locus_tag="QIE41_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
     gene            complement(41288..42121)
                     /gene="fdhD"
                     /locus_tag="QIE41_000394"
     CDS             complement(41288..42121)
                     /gene="fdhD"
                     /locus_tag="QIE41_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDS3529326.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            42315..45365
                     /gene="fdnG"
                     /locus_tag="QIE41_000395"
     CDS             42315..45365
                     /gene="fdnG"
                     /locus_tag="QIE41_000395"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:42900..42902,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDS3529327.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            45378..46280
                     /gene="fdoH"
                     /locus_tag="QIE41_000396"
     CDS             45378..46280
                     /gene="fdoH"
                     /locus_tag="QIE41_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDS3529328.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            46277..46912
                     /gene="fdoI"
                     /locus_tag="QIE41_000397"
     CDS             46277..46912
                     /gene="fdoI"
                     /locus_tag="QIE41_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDS3529329.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            46909..47838
                     /gene="fdhE"
                     /locus_tag="QIE41_000398"
     CDS             46909..47838
                     /gene="fdhE"
                     /locus_tag="QIE41_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDS3529330.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(48020..48262)
                     /locus_tag="QIE41_000399"
     CDS             complement(48020..48262)
                     /locus_tag="QIE41_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529331.1"
                     /translation="MNSLAGIDMGRILLNLSNELFKQLDDLEVQRNLPRAELLREAVD
                     QYLVNQSQTARASAFGIWQGSEEDGVEYQRKLREEW"
     gene            complement(48481..48699)
                     /locus_tag="QIE41_000400"
     CDS             complement(48481..48699)
                     /locus_tag="QIE41_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDS3529332.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(49118..49396)
                     /locus_tag="QIE41_000401"
     CDS             complement(49118..49396)
                     /locus_tag="QIE41_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529333.1"
                     /translation="MSTLNTRKDRDRNGKAKLHNHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(49448..49669)
                     /locus_tag="QIE41_000402"
     CDS             complement(49448..49669)
                     /locus_tag="QIE41_000402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter ATP-binding protein"
                     /protein_id="HDS3529334.1"
                     /translation="MTRNRAERRHHMARLKKSAAVIALQAMEAKKHQVFAFVLPVFVL
                     VGCVGIDAIIMVPAYRKFVQKHAMRIKGK"
     gene            complement(49754..50044)
                     /locus_tag="QIE41_000403"
     CDS             complement(49754..50044)
                     /locus_tag="QIE41_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3529335.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRAS
                     GLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(50045..50356)
                     /locus_tag="QIE41_000404"
     CDS             complement(50045..50356)
                     /locus_tag="QIE41_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDS3529336.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            50585..51493
                     /locus_tag="QIE41_000405"
     CDS             50585..51493
                     /locus_tag="QIE41_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS3529337.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMILRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QYRVIAVQYRLAPEQTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(51661..53475)
                     /locus_tag="QIE41_000406"
     CDS             complement(51661..53475)
                     /locus_tag="QIE41_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529338.1"
                     /translation="MVANINLIKQESYSIVNLEKQLMSNESPKQTLLNDFSHRLKMEG
                     EEQLCDCVNELKLGLSSNNKYDLFKNKETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEGMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVGEDILA
                     LPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIF
                     DLRFLSAIKQKVFLGSEKTLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAK
                     TVAAPIMATGVGSALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQLDISARNKYR
                     PAFDKWNELVDKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTIS
                     IVLGNSMLQTLLEKRLADGREIEKLFDQQGYFSCDSLTAKELMMLKDLFDIRVLHLSN
                     KFNVGLQGNINTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            complement(53891..54832)
                     /gene="fabY"
                     /locus_tag="QIE41_000407"
                     /gene_synonym="yiiD"
     CDS             complement(53891..54832)
                     /gene="fabY"
                     /locus_tag="QIE41_000407"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDS3529339.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(54877..55314)
                     /gene="dtd"
                     /locus_tag="QIE41_000408"
     CDS             complement(54877..55314)
                     /gene="dtd"
                     /locus_tag="QIE41_000408"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDS3529340.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(55311..56183)
                     /locus_tag="QIE41_000409"
     CDS             complement(55311..56183)
                     /locus_tag="QIE41_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDS3529341.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSMQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(56177..56776)
                     /gene="yihX"
                     /locus_tag="QIE41_000410"
     CDS             complement(56177..56776)
                     /gene="yihX"
                     /locus_tag="QIE41_000410"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDS3529342.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(56875..57660)
                     /locus_tag="QIE41_000411"
     CDS             complement(56875..57660)
                     /locus_tag="QIE41_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDS3529343.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(57694..58590)
                     /gene="yihV"
                     /locus_tag="QIE41_000412"
     CDS             complement(57694..58590)
                     /gene="yihV"
                     /locus_tag="QIE41_000412"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDS3529344.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGNLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            58758..59654
                     /gene="yihU"
                     /locus_tag="QIE41_000413"
     CDS             58758..59654
                     /gene="yihU"
                     /locus_tag="QIE41_000413"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDS3529345.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            59678..60556
                     /gene="yihT"
                     /locus_tag="QIE41_000414"
     CDS             59678..60556
                     /gene="yihT"
                     /locus_tag="QIE41_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDS3529346.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRRINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            60572..61813
                     /gene="yihS"
                     /locus_tag="QIE41_000415"
     CDS             60572..61813
                     /gene="yihS"
                     /locus_tag="QIE41_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDS3529347.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYAIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            61881..62783
                     /locus_tag="QIE41_000416"
     CDS             61881..62783
                     /locus_tag="QIE41_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDS3529348.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQDYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDDYLFQLPAEQVYAVDEHANPTTLHHVDELNLNFTRATKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            62844..63719
                     /locus_tag="QIE41_000417"
     CDS             62844..63719
                     /locus_tag="QIE41_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDS3529349.1"
                     /translation="MDFFTLFSPDGAMVETSVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRNWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            63827..65863
                     /gene="yihQ"
                     /locus_tag="QIE41_000418"
     CDS             63827..65863
                     /gene="yihQ"
                     /locus_tag="QIE41_000418"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDS3529350.1"
                     /translation="MDTPRPQLPDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLRISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTDSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEQGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            65909..67294
                     /locus_tag="QIE41_000419"
     CDS             65909..67294
                     /locus_tag="QIE41_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529351.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQIGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTE"
     gene            67335..68738
                     /locus_tag="QIE41_000420"
     CDS             67335..68738
                     /locus_tag="QIE41_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529352.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     SGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            68805..69497
                     /gene="ompL"
                     /locus_tag="QIE41_000421"
     CDS             68805..69497
                     /gene="ompL"
                     /locus_tag="QIE41_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDS3529353.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(69589..70854)
                     /locus_tag="QIE41_000422"
     CDS             complement(69589..70854)
                     /locus_tag="QIE41_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529354.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(70956..71936)
                     /locus_tag="QIE41_000423"
     CDS             complement(70956..71936)
                     /locus_tag="QIE41_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDS3529355.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(71944..72654)
                     /locus_tag="QIE41_000424"
     CDS             complement(71944..72654)
                     /locus_tag="QIE41_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDS3529356.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KKRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(72871..74694)
                     /gene="typA"
                     /locus_tag="QIE41_000425"
     CDS             complement(72871..74694)
                     /gene="typA"
                     /locus_tag="QIE41_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDS3529357.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            75067..76476
                     /gene="glnA"
                     /locus_tag="QIE41_000426"
     CDS             75067..76476
                     /gene="glnA"
                     /locus_tag="QIE41_000426"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDS3529358.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            76650..77699
                     /gene="glnL"
                     /locus_tag="QIE41_000427"
     CDS             76650..77699
                     /gene="glnL"
                     /locus_tag="QIE41_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDS3529359.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            77711..79120
                     /gene="glnG"
                     /locus_tag="QIE41_000428"
     CDS             77711..79120
                     /gene="glnG"
                     /locus_tag="QIE41_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDS3529360.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            79232..79342
                     /locus_tag="QIE41_000429"
     CDS             79232..79342
                     /locus_tag="QIE41_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDS3529361.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(79532..80905)
                     /gene="hemN"
                     /locus_tag="QIE41_000430"
     CDS             complement(79532..80905)
                     /gene="hemN"
                     /locus_tag="QIE41_000430"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDS3529362.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RKVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFILALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKHYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(81094..81603)
                     /gene="yihI"
                     /locus_tag="QIE41_000431"
     CDS             complement(81094..81603)
                     /gene="yihI"
                     /locus_tag="QIE41_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDS3529363.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
BASE COUNT        20059 a        21058 c        20482 g        20061 t
ORIGIN      
        1 cccgggatgt tgaaacgctt gttgaagcgg tcaacacggc caccggtagc aacatcacgc
       61 tgtttgccag tgaagaacgg gtggcacttg ctgcacacgt cgaggttcag gtcatgacca
      121 acggtggagc ggattttcat tacgttaccg caagagcagc tagcagtaat ttcttcgtat
      181 ttcgggtgaa tatctttttt catgggaaaa cctcggttta aggccgcgtc gctcttccag
      241 ccctaacgcc agacaccacg cgatgttaaa agtatagctt caatacgatc atttcgtacg
      301 aaggcgcgaa atcatacaga aattaaccag cgtatgcaaa ctgatccgca ctcttctacg
      361 gcaatgtgta tactaaccca ccgaatttca agtcaggatg atgctatgcc cgttgcccac
      421 gttgccttgc ccgttccgct tccccgtacc tttgactatc tgctgccaga aggcatgacg
      481 gtcaaagccg ggtgtcgcgt gcgcgtgccg tttggcaaac agcaggagcg catcggggtt
      541 gtggtgtcag ttagcaatgt cagcgagctg ccgcttaacg agctgaaagc ggtagttgaa
      601 gtgctggacg ttgagccggt atttactcac tccgtctggc gattactgct ctgggcggca
      661 gattactatc atcatccgat tggcgatgtg ctgtttcatg ccttgccgat tttactacgc
      721 caggggcggc ctgcggcgaa cgcgccgatg tggtactggt ttgccactga acaaggtcag
      781 gcggtggatc tgaacagcct gaaacgctca cccaagcaac aacaggcgct ggcagcgtta
      841 cggcaaggca aaatctggcg cgaccaggtt gccatgctcg aatttaatga tgccgcgttg
      901 caggcgctgc gcaaaaaagg tctgtgtgat ttagcaagtg aaacaccaga gtttagtgac
      961 tggcgaaaga attatgccgt ttctggtgag cggttgcgat tgaatactga acaggccacc
     1021 gccgttggcg caattcatag tgcggcggat accttctctg cctggctgct ggcgggcgtt
     1081 accggttccg gtaaaacgga ggtttatctc agtgtactgg aaaacgtgct cgctcagggc
     1141 aaacaggcgc tggtgatggt gccggaaatc ggcctgacgc cgcaaactat cgcccgtttt
     1201 cgtgaacgtt ttaatgcccc cgtggaagtt ctgcattccg gcctgaacga cagcgagcgt
     1261 ctttcggcgt ggttgaaagc gaaaaatggc gaggcggcga ttgtcatcgg cacccgctcc
     1321 gcgctgttta cgccgtttaa aaatctcggc gtgattgtta ttgatgaaga gcacgacagt
     1381 tcctacaaac agcaggaagg ctggcgctat catgcccgcg acctggcggt gtatcgcgcg
     1441 catagcgagc aaatcccaat tattcttggc tccgcaacgc ccgcactgga aacgttatgc
     1501 aacgtgcagc agaaaaaata ccgcctgctg cgcctgacgc atcgggcggg gaatgcgcgt
     1561 ccggcaattc aacacgtgct ggatttaaaa ggtcagaagg tgcaggctgg tctggctccg
     1621 gcgttaatca cccgtatgcg ccagcattta caggcaaaca atcaggttat cctctttctt
     1681 aaccgccgtg gctttgcgcc tgccttgctg tgccacgact gcggctggat tgccgaatgt
     1741 ccacgttgcg atcactacta cacgctgcat caggcgcaac agcatttgcg ctgccaccat
     1801 tgcgacagtc agcgtccggt gccgcgccag tgcccttcct gcggttccac gcacctggta
     1861 cccgtggggc tgggcaccga acagcttgaa cagacactcg cgccgttgtt ccctggtgtc
     1921 cccatttctc gtatcgaccg cgataccacc agccgcaaag gcgcactgga acaacaactg
     1981 gcggaagtac atcgcggtgg cgcgaggatt ttgatcggca cgcaaatgct ggcgaaaggt
     2041 caccactttc cggatgtgac gctggtggcg ttactggacg tagacggcgc gctgttttct
     2101 gccgattttc gctcggcaga gcgtttcgcc cagctttaca cccaggtcgc cggtcgtgcc
     2161 gggcgtgcgg gtaaacaggg cgaagtggtg ctgcaaacgc accatccgga acatcctctc
     2221 ttgcaaacat tgctctataa aggctacgac gcctttgccg aacaggcgct ggctgaacga
     2281 cgcatgatgc aactgccgcc gtggaccagc catgtgattg tgcgtgcgga agatcataac
     2341 aatcagcacg cgccgctgtt cctgcaacaa ctgcgtaatc tgatcctctc cagcccgctg
     2401 gcagacgaca aactgtgggt tctcggtccc gttccggcac tggcacctaa acgtggcggc
     2461 cgctggcgct ggcaaatttt gttgcagcac ccttcccgcg tgcgattaca gcacatcatc
     2521 aatggtacgc tggcgcttat taatacaata ccggattccc gtaaggtgaa atgggtgctg
     2581 gatgtcgatc ccatcgaggg ctaatgcgct cacgatgcga ggcggatcga aaaattcaat
     2641 attcatcaca cttttcatga aaattctgta accgttttca cgcgctatct gctaaaaatg
     2701 ttgccgatgt gaagtaaaca tggaagtagt acgcctgacg tgccaggcga ggagtgagtg
     2761 tgaaagcgaa gaagcaggaa actgccgcga ccatgaaaga cgttgccctc aaggcaaaag
     2821 tctctacagc gactgtctcc cgagcattaa tgaatcccga taaagtctcc caggccaccc
     2881 gtaatcgggt tgaaaaagcg gcccgggaag tgggttattt accgcagcct atggggcgca
     2941 acgtcaagcg taatgaatcc cgcaccattc tggtgattgt cccggatatc tgcgatccct
     3001 tctttagcga aattattcgc ggtatcgaag ttacggcggc aaatcacgga tatctggtgc
     3061 tgattggcga ctgtgcgcat caaaatcagc aggaaaaaac ctttatcgat ttgatcatca
     3121 ccaagcaaat tgatggcatg ttgttgctgg gttcaaggct gccgtttgat gccagcattg
     3181 aggaacagcg taatctgccg ccgatggtga tggcgaacga atttgcaccg gaactggagc
     3241 tgcctaccgt tcatatcgac aatctgaccg ccgcatttga tgcagtaaat tatttatatg
     3301 agcaagggca taaacggatt ggctgtatag ccggtcccga agagatgccg ctgtgtcact
     3361 accgcctgca aggctatgtt caggcgctgc gtcgctgcgg cattatggtt gatccgcaat
     3421 acatcgcccg tggcgacttc accttcgaag ccggaagcag agcaatgcaa cagctgcttg
     3481 atcttccaca accgcctact gctgtcttct gccatagcga tgtgatggcg ctcggcgcac
     3541 tttctcaggc aaaacgtcag gggctgaaag tcccggaaga cctttccata atcggttttg
     3601 ataacatcga cctgacgcaa ttttgtgatc cgccgctgac aaccatcgcg cagccgcgtt
     3661 acgaaatcgg tcgggaagct atgctgttat tgcttgatca aatgcagggg caacacgttg
     3721 gcagtggctc tcgtttaatg gactgcgaac ttatcatccg gggatcaaca cgcgcgttac
     3781 cttaaagtaa acctttaaga ctgccgtgac tggtcaaagg cccgccgctt aagtaacatg
     3841 gcgggctgac gaacgaataa atacagcgaa acgatagtgg cacaacgaga ttatgtacgc
     3901 cgcagccaac cggcaccttc gcggcgaaaa aagagcacct cacggaaaaa gcaacgaaat
     3961 ctgcctgcgg tttctcccgc tatggtcgct attgctgccg ccgttcttgt gacctttatc
     4021 ggtggtctgt acttcattac gcatcacaag aaagaagagt ccgagacgct gcaaagccag
     4081 aaagtgaccg gaaacggact accaccaaaa ccagaagaac gctggcgcta cattaaagag
     4141 ctggaaagtc gccagccggg tgtgcgtgcg cccacagaac cttctgccgg tggtgaagtg
     4201 aaaacgccgg agcaactgac accagaacaa cgccagcttc ttgaacaaat gcaggctgat
     4261 atgcgccagc agccaacgca gctggttgaa gttccgtgga atgagcagac gccggaacag
     4321 cgtcagcaaa cgctacagcg ccagcgtcag gcgcagcaat tagcggagca gcaacgcctg
     4381 gctcagcagt cgcgtacgac tgaacaaagc tggcagcagc agacgcgtac gtcgcaagcc
     4441 gcaccggtac aggcgcaacc gcgccagtct aaaccggctt ctactcagca gccgtaccag
     4501 gatctgctgc aaactcctgc gcacacgacc gcgcagtcga agccgcagca agctgcgccg
     4561 gttgctcgtg ccgctgacgc gccaaaaccg acggcggaga aaaaagacga acgccgctgg
     4621 atggtgcagt gcggttcgtt cagaggcgcg gaacaggcag agacggtacg tgctcagctg
     4681 gcgtttgaag gctttgactc gaagatcacc accaacaatg gctggaatcg tgtggtcatt
     4741 ggcccggtga aaggcaaaga gaacgcagac agcaccctca atcgcttgaa gatggcgggt
     4801 catacaaact gcattcggct cgccgccggg ggttgaaacc ctcaaaatcc cccccatcta
     4861 taattgcatt atgccccgta cttttgtacg gggtttgtac tctgtattcg taaccaaggg
     4921 gtcagctcgt gacaactata gtaagcgtac gccgtaacgg ccatgtggtc atcgctggtg
     4981 atggtcaggc cacgttgggc aataccgtaa tgaaaggcaa cgtgaaaaag gtccgccgtc
     5041 tgtacaacga caaagtcatc gcgggctttg cgggcggtac tgcggatgct tttacgctgt
     5101 tcgaactgtt tgaacgtaaa ctggaaatgc atcagggcca tctggtcaaa gccgccgttg
     5161 agctggcgaa agactggcgt accgatcgca tgctgcgcaa acttgaagca ctgctggcag
     5221 tcgcggatga aaccgcttcg cttatcatca ccggtaacgg tgacgtggtg cagccagaaa
     5281 acgatcttat tgctatcggc tccggcggcc cttacgccca ggctgcggcg cgcgcgctgt
     5341 tagaaaacac tgaacttagc gcccgtgaaa ttgctgaaaa ggcgttggat attgcaggcg
     5401 acatttgcat ctataccaac catttccaca ccatcgaaga attaagctac aaagcgtaag
     5461 gatctcccat gtctgaaatg accccacgcg aaatcgtcag cgaactggat aagcacatca
     5521 tcggccagga caacgccaag cgttctgtgg cgattgctct gcgtaaccgc tggcgtcgca
     5581 tgcagctcaa cgaagagcta cgccatgaag tgaccccgaa aaatatcctg atgatcggcc
     5641 cgaccggtgt cggtaaaact gaaatcgccc gtcgtctggc taagctggcg aatgcgccgt
     5701 tcatcaaagt tgaagcgacc aaattcaccg aagtgggcta tgtcggtaag gaagtggatt
     5761 ctattattcg cgatctgacc gatgccgccg tgaaaatggt acgcgtccag gctatcgaga
     5821 aaaaccgtta tcgcgctgaa gaactggcag aagaacgtat tctcgacgtg ctgatcccac
     5881 ctgctaaaaa caactgggga cagaccgaac agcagcagga accgtccgct gctcgtcagg
     5941 cattccgcaa gaaactgcgt gaaggccagc ttgatgacaa agaaatcgag atcgatcttg
     6001 ccgcagcacc gatgggcgtt gaaattatgg ctcctccggg catggaagag atgaccagcc
     6061 agctgcagtc catgttccag aacctgggcg gccagaagca aaaagcgcgt aagctgaaaa
     6121 tcaaagacgc catgaagctg ctgattgaag aagaagcggc gaaactggtg aatccggaag
     6181 agctgaagca agacgctatc gacgctgttg agcagcacgg gatcgtgttt atcgacgaaa
     6241 tcgacaaaat ctgtaagcgc ggcgagtctt ccggtccgga tgtttctcgt gaaggtgttc
     6301 agcgtgacct gctgccgctg gtagaaggtt gcaccgtttc caccaaacac gggatggtca
     6361 aaactgacca cattctgttt atcgcttctg gcgcgttcca gattgcgaaa ccgtctgacc
     6421 tgatcccgga actgcaaggt cgtctgccaa tccgcgttga gctgcaggca ctgaccacca
     6481 gcgacttcga gcgtattctg accgagccga atgcctctat taccgtgcag tacaaagcac
     6541 tgatggcgac tgaaggcgta aatatcgagt ttaccgactc cggtattaag cgcatcgcgg
     6601 aagcggcatg gcaggtgaac gaatctaccg aaaacatcgg tgctcgtcgt ttacataccg
     6661 ttctggagcg tttaatggaa gagatttcct acgacgccag cgatttaagc ggtcaaacca
     6721 tcactattga cgcagattat gtgagcaaac atctggatgc gttggtggca gatgaagatc
     6781 tgagccgttt tatcctataa tcgcgttcaa tcattttcat cattgtttga tggggctgaa
     6841 aggccccatt tttattggcg cgtattatga ctgaacaaca aattagccga actcaggcgt
     6901 ggctggaaag tttacgacct aaaaccctcc ccctcgcctt tgctgcaatt atcgtcggga
     6961 cggcgctggc atggtggcaa ggtcacttcg atccgctggt cgccctgctg gcactgatta
     7021 ccgccgggct attacagatc ctttctaacc tcgccaatga ttacggcgat gcggtaaaag
     7081 gcagcgataa acctgaccgt atcggaccgc tacgcggcat gcaaaaaggg gtcattaccc
     7141 agcaagagat gaaacgggcg ctcattatta ccgtcgtgct catctgtctc tccgggctgg
     7201 cactggttgc agtggcgtgc catacgctgg ccgattttgt cggcttcctg attcttggcg
     7261 ggttgtcgat cattgccgct atcacctaca ccgtgggcaa tcgtccttat ggttatatcg
     7321 gtctgggtga tatttccgta ctggttttct ttggctggtt gagcgtcatg ggcagctggt
     7381 atttacaggc tcatacattg attccggcac tgatccttcc ggcgaccgca tgcggcctgc
     7441 tggcaacggc agtactgaac attaataacc tgcgtgatat caatagcgac cgcgaaaatg
     7501 gcaaaaacac gctggtggtg cgcttaggtg aagtgaacgc gcgtcgttat catgcctgcc
     7561 tgctgatggg ctctctggtg tgcctggcgc tgtttaatct cttttcgctg catagcctgt
     7621 ggggctggct gttcctgctg gcggcaccat tactggtgaa gcaagcccgt tatgtgatgc
     7681 gggaaatgga cccggtggcg atgcgaccga tgctggaacg cactgtcaag ggagcgttac
     7741 tgactaacct gctgtttgtt ttagggatat tcctaagcca gtgggcagca taactgacaa
     7801 atatcaatta acaattgatg attttgccaa cagcctacat agcgcgatat actgaaaatt
     7861 ctcgcagcaa ctgaatgtta agcctatgaa atacgatact tccgagcttt gtgacatcta
     7921 tcaagaagat gttaacgtcg tggaaccgct gttctccaac tttggcggac gggcgtcgtt
     7981 tggcggacaa ataatcacgg taaaatgttt cgaggacaac gggttgctgt acgatctgct
     8041 cgaacagaat ggccgtggtc gtgttcttgt cgtcgatggc ggtggttctg ttcgtcgcgc
     8101 actggtcgat gctgaactgg cgcgtctggc agtacaaaat gaatgggaag gtctggtcat
     8161 ttacggcgcg gtgcgtcagg tagatgacct ggaagagttg gatatcggca tccaggcgat
     8221 ggcggcaatt ccggttggtg ctgctggcga aggtattggc gaaagcgatg tccgcgtcaa
     8281 ttttggcggt gtcaccttct tctccggcga ccatctttat gccgacaata ccgggattat
     8341 tctttcagaa gatccgttgg atattgaatg atagaaaagg caccgcaagg tgcctttttt
     8401 ctgcgttacc tgttggccta cacagtaaag aaattacgcg gaagatgaag cgtaatcaga
     8461 cctcttccat gcgacccagc agggcctgca gacgttcctg ccagccgttc tgctgttctt
     8521 tcagatggtt gttctcacgc tccagctctt cgcgctgatg ctgggcattt tgaacttcct
     8581 gcgacagtga gttgtttttt tctttcagct cttcgatttc catctgcaac agagtgatgg
     8641 tatcaatcgc ctgctgtact tttgcttcca gtttctcaaa cacttctaat gacattgtca
     8701 tacctctcct gaattgcaag gcgttgatgg ataaaaatcc tcgtcccgat taccggtgac
     8761 gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg cgccatcgct
     8821 gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc tcctggtgac
     8881 ataatccaca tcaatcgaaa atgttaataa atttgttgcg cgaatgatct aacaaacatg
     8941 catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg acgaagcaca
     9001 cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta cagcatgcct
     9061 acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact cttcaggatc
     9121 cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc ctcggtaccg
     9181 ggttgttgat ttttttcggg gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt
     9241 ttggtcagtg ggaaatcagt gtcatttggg gactgggagt ggcaatggcc atctacctga
     9301 ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg tggctgtttg
     9361 cctgtttcga caagcgtaaa gttattcctt ttatcgtttc acaagttgcc ggcgctttct
     9421 gcgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc gagcagactc
     9481 atcacattgt tcgcggcagc gttgaaagtg ttgatctggc tggcactttc tctacttacc
     9541 ctaatcctca tatcaatttt gtgcaggctt tcgcagttga gatggtgatt accgctattc
     9601 tgatggggct gatcctggcg ttaacggacg atggcaacgg tgtaccacgc ggccctttgg
     9661 ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc ccattgacgg
     9721 gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct
     9781 ggggcaatgt cgcctttact ggcggcagag acattcctta cttcctggtg ccgctttttg
     9841 gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt
     9901 tgccttgcga tatctgtgtt gtggaagaaa aggagaccac aactccttca gaacaaaaag
     9961 cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaaata tatcgttgcg
    10021 ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt
    10081 agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac caggctgggt agaacacgac
    10141 ccaatggaaa tctgggccac ccaaagctcc acgctggtag aagtgctggc gaaagccgat
    10201 atcagttccg atcaaattgc agctatcggt attacgaacc agcgtgaaac cactattgtc
    10261 tgggaaaaag aaaccggcaa gcctatctat aacgccattg tctggcagtg ccgtcgtacc
    10321 gcagaaatct gcgagcattt aaaacgtgac ggtttagaag attatatccg cagcaatacc
    10381 ggtctggtga ttgacccgta cttctctggt accaaagtga agtggatcct cgaccatgtg
    10441 gaaggctctc gcgaacgtgc gcgtcgtggt gaattgctgt ttggtacggt tgatacgtgg
    10501 cttatctgga aaatgactca gggccgtgtc catgtgaccg attacaccaa cgcctctcgt
    10561 accatgttgt tcaacatcca taccctggac tgggacgaca aaatgctgga agtgctggat
    10621 attccgcgcg agatgctgcc agaagtgcgt cgttcttccg aagtgtacgg tcagactaac
    10681 attggcggca aaggcggcac gcgtattcca atctccggga tcgccggtga ccagcaggcc
    10741 gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga aaaataccta cggcactggc
    10801 tgctttatgc tgatgaacac cggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc
    10861 accatcgcct gtggcccaac tggcgaagta aactatgcgc tggaaggtgc ggtgtttatg
    10921 gcgggcgcat ccattcagtg gctgcgcgat gagatgaagt tgattaacga cgcgtacgat
    10981 tccgaatatt tcgccacgaa agtgcaaaac accaatggtg tatatgtggt tccggcattt
    11041 accgggctgg gtgcgccata ctgggatccg tatgcgcgcg gggcgatttt cggtctgact
    11101 cgtggagtga acgctaacca cattatccgc gcgacgctgg agtctattgc ttatcaaacg
    11161 cgtgacgtgc tggaagcgat gcaggccgat tctggtattc gtctgcacgc cctgcgcgtg
    11221 gatggcggcg cagtggcgaa caatttcctg atgcagttcc agtccgatat tctcggtacg
    11281 cgcgttgagc gcccggaagt gcgcgaagtc accgcattgg gtgcggccta tcttgctggt
    11341 ctggcggttg gcttctggca gaacctcgac gagctgcaag agaaagcggt gattgaacgc
    11401 gagttccgtc caggcatcga aaccactgag cgtaattacc gttacgcagg ctggaaaaaa
    11461 gcggtgaaac gcgcgatggc gtgggaagaa cacgacgagt aatgtaaatg ccggatgaag
    11521 cgtttatgcc gcatccggta gtcctgaatc gtgcgggggc aaccccgcac acatcaataa
    11581 tcccgccctt cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga
    11641 gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc agcggcgctg gctggctaca
    11701 aatggttagg acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta
    11761 ttatgctcaa ccaggtcaac attgacggca ccatcgttat tggtgaaggt gaaatcgacg
    11821 aagcaccgat gctctacatt ggtgaaaaag tcggtactgg gcgcggcgac gcggtagata
    11881 ttgctgttga tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg
    11941 cggtgctggc agtaggcgat aaaggctgtt tcctcaacgc gccagatatg tatatggaga
    12001 agctgatcgt cggaccggga gccaaaggca ccattgatct gaacctgccg ctggcggata
    12061 atctgcgcaa tgtagcggcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc
    12121 tggctaaacc acgccacgat gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat
    12181 ttgctattcc ggatggcgac gttgcggcct caattctcac ctgtatgcca gacagcgaag
    12241 ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt ggtttctgcg gcggtgatcc
    12301 gcgcattaga tggtgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca
    12361 acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg
    12421 aagccggtaa agtattgcgt ctgggcgata tggcgcgcag cgataacgtc atcttctctg
    12481 ccaccggtat taccaaaggc gatctgctgg aaggcattag ccgcaaaggc aatatcgcga
    12541 ctaccgaaac gctgctgatc cgcggcaagt cacgcactat tcgccgcatt cagtccatcc
    12601 actatctgga tcgcaaagac ccagaaatgc aggtgcacat cctctgattg atttgatcga
    12661 ttgagccttc cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa
    12721 gtcaatcaaa acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc
    12781 agaactggac cgacgccctg tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg
    12841 ccgggcaatt taccaagctt ggccttgaaa tcgacggtga acgcgtccag cgcgcctact
    12901 cctatgttaa ctcgcccgat aatcccgatc tggagtttta cctggtcacc gtccccgatg
    12961 gcaaattaag cccacgattg gcggcactga aaccaggcga tgaagtgcag gtggttagcg
    13021 aagcagctgg cttctttgtt ctggatgaag taccagattg cgaaacgcta tggatgctgg
    13081 caaccggtac agcgattggc ccttatttat cgattctgca actgggcaaa gatttagatc
    13141 gcttcaaaaa tctggtcctg gttcacgccg cacgttatgc cgccgactta agctatttgc
    13201 cactgatgca agaactggaa aaacgctacg aaggcaaact gcgcattcag acggtggtca
    13261 gtcgggaaac ggcagcgggg tcgctcaccg gacggatacc agcgttaatt gaaagtggtg
    13321 aactggaaag cgcggttggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg
    13381 gcaatccaca gatggtgcgc gatacgcaac agttgctgaa agagacccgg cagatgacga
    13441 aacatttacg tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt
    13501 atcgataaac ggcacgatga gcaaatccgc actcaactta ttgatcatcc cgcgatatgc
    13561 cggcatcaaa cggttgataa atgagtggtg atgaccacag acaaggaggt cgcactgctc
    13621 tttttgcata atttccagca gtgtttccgg catttctccg cgttcaatac gcagttttgt
    13681 cttcggccat tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa
    13741 caattgaaga atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc
    13801 atcatcaata tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagggcttt
    13861 attcaccagt aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc
    13921 catgtttaac tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa
    13981 ataatcatct cggcctggca tgagaaggtg aaggccgcat caagctcaac ttgcccctcc
    14041 agatccttaa tcttataggc cacgctggcc ccttcatctg cagaggatgc agcggcggca
    14101 agctctttca gtttggtcat aaaggactct gcttcttcgc gggtggcaaa aaagcgcgaa
    14161 aacttactgg tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta
    14221 gtactgcatt tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg
    14281 attacgactt aagaataatg cgccgttaag ggaagaacaa acaagtaatg atcttatgga
    14341 ttaccatttt ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggtattgt
    14401 catttgcatc gtggaaataa caatcccgga aaaaaatagc agtaagagat atattaaagg
    14461 ttaattactt gatttattgt cggctttata cttcacatcc tcggtatctt taccatattt
    14521 attttcgcct tgtgtgccaa caaacgcacc aagatcgata agcatcatca ccagaatcaa
    14581 cgtcgggaca aaacgcccca ccgcccattg ccagacaccc ggtaaaatcg cccagttacc
    14641 cgccagcagc atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc
    14701 atgcaagcgc ttaacggtta ctgccgctgt tggccagagc aagcacacaa ggcaaaacgc
    14761 cgcagtctga atatcgagta aatttttacc cgccagtgag aaaagcacca gcatgcctgc
    14821 gaaccacagg cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa
    14881 taaccattgc tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc
    14941 ttttgacaag ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc
    15001 aatgaagcca gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac
    15061 ggcgcatgca caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc
    15121 tactttcgat ctctccatca gccagtttag agaagacttt aacagccaga atcccagcct
    15181 gccactgaac gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc
    15241 tgccagtaaa attaatgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa
    15301 aatcaaaagc attcaaatga cctggctacc catccagggg ccagagcaaa aagccgcgaa
    15361 agcgaaagct caggaataca tggcagcggt gatccgcaca ctcaccccat taatgaccaa
    15421 aacacaaagc cagaaaaaac tgcagtcgct actaacggcg gggaaaaaca aacgttatta
    15481 caccgagaca gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac
    15541 cttcgctgtt gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg
    15601 acaaaaagca aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct
    15661 gcggggcggc catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct
    15721 ttagtgatgg gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct
    15781 aacctgcgta aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa
    15841 atgtacatcg atatggcgaa gcgtgaagct gaaggcagcc acatcatgct gggtgcgcaa
    15901 aacgtggacc tgaacctgtc cggcgcattc accggtgaaa cctctgctgc tatgctgaaa
    15961 gacatcggcg cacagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag
    16021 tctgacgaac tgatcgcgaa aaaattcgcg gtgctgaaag agcagggtct gactccggtt
    16081 ctgtgcatcg gtgaaaccga agctgaaaac gaagcgggca aaactgaaga agtttgcgca
    16141 cgtcagatcg acgcggtact gaaaactcag ggtgctgcag cattcgaagg tgcggtaatc
    16201 gcttacgaac ccgtatgggc aatcggtact ggcaaatctg caactccggc gcaggcacag
    16261 gctgttcaca aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa
    16321 gtgatcatcc agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag
    16381 ccagacatcg acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagtg
    16441 atcgttaaag ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata
    16501 tccggcactt actttcctta actcctcgcc ttaacgcaaa atctcacact gatgatcctg
    16561 gatttcctcg gctgaagcac ggttcagcgt cagtagattt cgttgtgtcg ccagcaaaac
    16621 aaacgaatta tcactctgcc gcaccatcgc cagcccgtag cttcccatat gttcccgcgc
    16681 ctcaggcact tcctctgcaa gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt
    16741 tacccgacgc gccaggtact catgcccacg caaaccacct ggcagtggca accagcggct
    16801 gctgatgtta gccagattgt tatccagctg ttcgcgcaca tcatgacgaa tacaagagat
    16861 atgaatatga aaatggtttt gcgtacgccc ggtgcgagag ttgatcgcca aagaaaccgc
    16921 gcgatcggga accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa
    16981 ccaaaagaag ttcggcgttg aaggatcggt caacaaagga ctttccgtgc cgttaatacg
    17041 atacgttggc atcaacagat attgcagtgg gccattaaga tcttttaaaa ccacgtatcc
    17101 ggcattgggt ttgacttccg cacatggcga aggattttga ttttgctgtt gattgggcaa
    17161 acattcctca aggacaatct tacgtagtgt atccgactct tcaccggtta atttccagta
    17221 accaataccg gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt
    17281 tttcatcttt ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa
    17341 taaaaaaccc ggtcgcgaga aaccaccggg tttgataatt atcctgggga aatcagcgtt
    17401 tgctgatctg atcgaacgta ccgccgttag caaaatgctc tttttgcgct ttcgtccagc
    17461 cgccgaactc ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt
    17521 tcgccacctc agcatcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg
    17581 gcgagtagag atatttcaga taggcttccg ccacctcttt ggtgcctttt ttctcgacca
    17641 ctttatcgac caccgacacg gttggctctg cgaggataga ctcactcggc gtaacgattt
    17701 cgaatttatc tttccccagc tcattcgctg ccagcagagc ttcgttttcc caggcgatca
    17761 gtacatcgcc aatcccgcgc tcgacaaagg tattagtgga gccgcgcgcg ccagaatcca
    17821 gaacttcgac gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt
    17881 tgttgtgatg cagcgcgtag ccccaggctg ccaggtagtt ccagcgcgcg ccaccagagc
    17941 ttttcggatt aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga
    18001 tctgcttcgg attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt
    18061 tatccggcag acgtttgatc cactctttat caatccgccc gcgttccgca atcgcgtcca
    18121 cgtcataggc cagagccaga gtgacaacat cagcttcaat accattgatt accgacgtcg
    18181 cttgtttgcc tgaaccaccg tgtgactgac gaatcaccac gttatcgcca gtttgctgtt
    18241 tccagtgggc gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg
    18301 aaacgttaag aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta
    18361 accctacgcc ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc
    18421 gtgccagaag gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgcgggta
    18481 tatgttgggg gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtgcgctt
    18541 tgtccatgcc ggatgcggca caaacgcctt atccgaccta taaaagtttg caaattcaat
    18601 agattacaga attcatgtag gcctgataag cgaagcgcat caggcctttt tgcttctgtc
    18661 atcggtttca ggctaaagga atctgccttt ttccgaaatc attaatacag ttttttcgcg
    18721 cagtccagcc agtcgccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg
    18781 tggtgaacca gctgttcgtt ctggatacct acgcaacgac cgccgtaacc tgccagcagc
    18841 agatcgatag cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa
    18901 ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg
    18961 atgaaatgcg ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt
    19021 tttttacctt tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca
    19081 acttccggaa ccacaacgaa ttcacagcca ccggcaatgg ccgcagccag ggtcagatcg
    19141 ccacaataac ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca
    19201 cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag
    19261 tcagtgcctt tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt
    19321 tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg
    19381 ataccacgtt ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc
    19441 gggaaacgcg cagaaccgag gaacgtacca ccacggttga tcatgtcaga aacgctgtaa
    19501 cggtctagct gtaccatacg gtcttcatac agacccagat agccgtcata aatgcccatt
    19561 acttccagac cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct
    19621 ggcgcatcac cgccgcttgt caacacaccg attttcttaa tcatgactac ctctgaactt
    19681 tggaatgcaa aatgaaatct gttgccggaa gtcttcttgc acatcgaagt gatccaacga
    19741 atgtgcaaat agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg
    19801 gtattttata cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac
    19861 cgtttaccct ccctgggtac gacggaacag gggtcctgat ggataatcac atccgatccc
    19921 ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa
    19981 ggcagagagt cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc
    20041 gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct
    20101 tgccgttctt catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg
    20161 cgtaacgcgc tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga
    20221 tgccagccgt accaggacaa ccccagcgcc agcagaattg cgccgttcat cataacatca
    20281 gactggtaat gtagcatatc agcccgcacc gcctggcttt gcgtccggcg caccacccac
    20341 cgctgaaacg agacaaggat aatcgtacaa attagcgcca caattgtcac gattaccccg
    20401 acgcctggat ctgtcatcgg tgttggagat atcagatgtt gaatacccgt caaaaagagg
    20461 aatagtgccg aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctcagcttta
    20521 ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccagtaaa
    20581 ttcgtcaacg acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc
    20641 gacccggtat accaccatgc aaaaatttta atcagcaata gcagtgaagc catcgccgtt
    20701 gcagcaatcg ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct
    20761 atcaactgac gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa
    20821 ttgaggataa aaaaaacccc cacatcatgt gggggaagac agggatggtg tctatggcaa
    20881 ggaaaacagg gtttactact gggaacgtga gttgctacta ctcaatagct tcaacgatga
    20941 acttttttgc cattgcgtca cgtcacgcaa ctgctccatt cgttgttgat gtttctcatt
    21001 taaaaccgct tgctgctccg gcgttaacag gcgatacatt tggttgcgga cttttgccat
    21061 ctcaacctga cgagcaattt gctcattcgc cattttttct gcctgtgcgc gcacagcgtt
    21121 ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc tccagttcgc taacattaac
    21181 aggaggctgt tcgtgccggg cctgttgcat aagatctcgc atctgctgac gctgatgttc
    21241 ggttaaactt atgccgtcga acatatggct ctgcgtactg cgctgcgtaa gttcttcacc
    21301 cggatgccag ttatcgactg aaccgacttc agcagcgtgg cttaatgaac tgactgccag
    21361 cgttgaggcc atgacggcag cggtaactat gcgcatcatt tgctcccaaa atctttctgt
    21421 cgcgattcaa cgatagagag tttacgattc aggctgcaaa catgcgtcag ggggtgtaaa
    21481 acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa tttctgcctc ggaggtattt
    21541 aaacaatgaa taaaatcctg ttagttgatg atgaccgaga gctgacttcc ctattaaagg
    21601 agctgctcga gatggaaggc ttcaacgtga ttgttgccca cgatggggaa caggcgcttg
    21661 atcttctgga cgacagcatt gatttacttt tgcttgatgt aatgatgccg aagaaaaatg
    21721 gtatcgacac attaaaagca cttcgccaga cacaccagac gcctgtcatt atgttgacgg
    21781 cgcgcggcag tgaacttgat cgcgttctcg gccttgagct gggcgcagat gactatctcc
    21841 cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg cgcgatcctg cgccgttcgc
    21901 actggagcga gcaacagcaa aacaacgaca acggttcacc gacactggaa gttgatgcct
    21961 tagtgctaaa tccaggccgt caggaagcca gcttcgacgg gcaaacgctg gagttaaccg
    22021 gtactgagtt taccctgctc tatttgctgg cacagcatct gggtcaggtg gtttcccgtg
    22081 aacatttaag ccaggaagtg ctgggcaaac gcctgacgcc tttcgaccgc gctatcgata
    22141 tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa agatggtcac ccgtggttta
    22201 aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc atgataggca gcttaaccgc
    22261 gcgcatcttc gccatcttct ggctgacgct ggcgctggtg ttgatgttgg ttttgatgtt
    22321 acccaagctc gattcacgcc agatgaccga gcttctggat agcgaacagc gtcagggtct
    22381 gatgattgag cagcatgtcg aagcggagct ggcgaacgat ccgcccaacg atttaatgtg
    22441 gtggcggcgt ctgttccggg cgattgataa gtgggcaccg ccaggacagc gtttgttatt
    22501 ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc gaaatgcaga tcattcgtaa
    22561 ctttattggt caggccgata acgccgatca tccgcagaag aaaaagtatg ggcgcgtgga
    22621 actggtcggt ccgttctccg tgcgtgatgg cgaagataat taccaacttt atctgattcg
    22681 tccggccagc agttctcaat ccgatttcat taacttactg tttgaccgcc cgctattact
    22741 gctgattgtc accatgttgg tcagtacgcc gctgctgttg tggttggcct ggagtctggc
    22801 aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt gcccagggaa acttacgcca
    22861 gcacccggaa ctggaagcgg ggccacagga attccttgcc gccggtgcca gttttaacca
    22921 gatggtcacc gcgctggagc gcatgatgac ctctcagcag cgtctacttt ctgatatctc
    22981 tcacgaactg cgcaccccgc tgacgcgtct gcaactgggt acggcgttac tgcgccgtcg
    23041 tagcggtgaa agcaaggaac tggagcgtat tgaaaccgaa gcgcaacgtc tggacagcat
    23101 gatcaacgat ctgttggtga tgtcacgtaa tcagcagaaa aatgcgctgg ttagcgagac
    23161 catcaaagcc aaccagttgt ggagtgaagt gctggataac gcggcgttcg aagccgagca
    23221 gatgggcaag tcgttgacgg ttaacttccc gcctgggccg tggccgctgt acggcaaccc
    23281 gaacgccctg gagagtgcgc tggaaaacat tgttcgtaat gccctgcgtt attcccatac
    23341 gaagattgaa gtgggctttg cggtagataa agacggtatc accattacgg tggacgacga
    23401 tggtcctggc gttagcccgg aagatcgcga acagattttc cgtccgttct atcgtaccga
    23461 tgaagcacgc gatcgtgaat ctggcggtac aggtttgggg ctggcaattg ttgaaaccgc
    23521 cattcagcag catcgtggct gggttaaagc ggaagacagc ccgctgggcg gtttacggct
    23581 ggtgatttgg ttgccgctgt ataagcggag ttaaactcgg cgcagagccg tccccttcgg
    23641 ggtgaaggca acaggtcagc actgtgcgaa ctgataccct caccccggcc ctctccctga
    23701 aagggcgagg gggaaaaacg tagccctgtt cagcaacgga gctgagcacc gctccggaca
    23761 gtcccctcgc ccctctgggg agagggttag ggtgagggga aaaattcagc acaatgcaaa
    23821 cggcactgct ctcaccggaa accatgccag atttcgtatt aaggcagact tattttcccc
    23881 acaaccggcg ggaaaaatct tcgatcttgc cgctcagtcg gcgcttttgc atcgtccgcg
    23941 tccagctttt cgataacccg gcagcggaga gtaaacggtg atactgctcg tcatcaaacg
    24001 gcatatgcca tgcgatggcg gcagcctcct gcacggtcac atcactgaca cgggaaacca
    24061 actccagcgg agcatcagca gataccaacc ctggcgcaat cacgctatac aaccacccca
    24121 ctttacccgt gttttgcatc aactgcgcta tatcgctgat atcaaaatgg taattgagtt
    24181 tatagcaagg tgagcgtggc tggctgacct gaattaatgc ctctccccag cggaaaatat
    24241 cgccaatata aacattactt tccgtcagac cgtcggttga gagattttcg ccaaacgcag
    24301 gcgcaacaaa caactccgcc tgttccggaa attcccgcgc ccagtaaaga taatgctcac
    24361 gaggataatg acacagcgct ctgtctggcc cgccgtgaac ctttttctcc gcctgctcgt
    24421 caccttccag ccccagctcc gtcagcatca actcaccatc aacctggatt ttggcaattg
    24481 cgctgggttt gccttcggga taagcctgaa tcttgcctgt gtatacatca accggatatc
    24541 gcatctgttc cctctccacg agaaatttcc ccattagacc atatttattt tatgtcaggc
    24601 gtgctgtttt cgcggtaagt atccaggcag gaactcaata gcaaaccgga cgaacgttag
    24661 ttcatccggc tgtggggatt aaggaagcag gtttatttca ccgtacctaa aatttcgatt
    24721 ttcgctgctc tggctttgac tttacgtgac cagatagagg taagaattgg caccagaatc
    24781 gaagtcacaa tgaccgccgt cgcgaccagc gaagttgctg ccggagccat cggtttaaac
    24841 gcaggcacca tttctgcaat cagcacaggt gtcgctaccg cggcccctgc ggaactggaa
    24901 gcggcaattc cggcagtgcc atcgccaccg ccaatcaatt tatcagcgat aatcaacgga
    24961 ataccggtca cgataattac tgccacaccc aacaggatcc ccagcaaacc tgtctgggca
    25021 attacagtca aatcaatggt attacccagt gcgaaggcaa agaatggaat cagcgtctgc
    25081 accgctttgc tgaaaaattc tcgtaattca gggtcaaggt tcccaagggc aaagcccacc
    25141 agaaacggta atactgcgcc gacgaaaaca tgcggttcaa acgaggcaat cccggcagtg
    25201 cccagaataa tcatcgtcat gagcggcccg gactccaatg acatcaacac aaatgcccca
    25261 gcttcttctt ttgtgccgta ctgctgcatg atggaagcgt aaagtccgcc gttggtcata
    25321 tccatcgccg ccaccagcgc cagcgttgaa agtccggcaa agaatccaac ttcaacacca
    25381 tgttccggaa taatgcgcga ggcaattgcc gcaaccaccc acgcgacggc aattttagtt
    25441 accaccagcg taccggattt acgcaatacc gttcccgtcg cgcttaattt tattgacgcc
    25501 cccatgcaaa aaaaccacac cgccagaatg ggcaccgtac cggtaatcat cccgttggtg
    25561 aatgatccaa aatatttccc cgcctccggc gagaaggtgt ggcacagtgc gccaaggaat
    25621 agcgggacga gcatcatccc ccccgggatt ttctcaatcg agcgttttat ctgcatttcc
    25681 atcacctgtc ggcatatttt taagggctga tgcgtcattc ctcaatggag catcagtgtt
    25741 gatggaaacg aagatagaca ttcatttaat caataaaagt gatcaaaacc acatcaattg
    25801 aaacactgtt ttatttttat cggatcattg tttttatttt gaaattgatc acaaaaaaac
    25861 caccgccgtt ggcgatggtt cattttttaa gctgatatgc ggcgtaaacg cctcattgca
    25921 gcaggcggca aatgattatt ttttcgccgc gaaacgtgcc gctgcttcgt cccagttcac
    25981 cacgttccag aactctttga tgtagtcagg acggcggttc tggaatttca ggtagtaagc
    26041 atgttcccac acatccaggc ccagaatcgg gaagccggaa gcgccagaaa tagcttcacc
    26101 catcagcggg gaatcctggt tagcagtaga aaccaccgcc agtttatcgc ctttcagcac
    26161 cagccatgcc cagccggaac caaagcggga agctgccgct ttctcaaatt ctgctttgaa
    26221 gttatcaacg gagccgaagt cacgttcgat agccgctttc aggtcaccct gtaaggtggt
    26281 gccttttttc agacctttcc agaacaggct gtggttagcg tgaccgccag cgttgttgcg
    26341 cagtactgtt ttcttgtctg ctggcagctg gtccagtttg gtgatcagct cttcaaccgg
    26401 caggttggca aattctggca ggctttccag cgccgcgttg gcgttgttga cataggtctg
    26461 atggtgtttg gtgtggtgga tttccatggt ctgcttatcg aagtgcggtt ccagggcatc
    26521 gtaagcatac ggcagggatg gcagggtata gctcatattc atctccagta ttgtcgggcg
    26581 gccgattgtt aatgccgcgt aagcagttgg ttcattatag ttaattaaat gatattgaaa
    26641 atgattatca atgccgtact tttcgtaagg gtatggtttt gcaggaaaat gcccgagatg
    26701 tgaagcaaat cacccactta atgccgtgat tgccagtaaa tcgacaacgg cggcaacagg
    26761 cgaaaggtta atcgacagca cgatttttac actcatctcg tcggagatgt gacgcgacga
    26821 aaaatgatga ggataagaag atgagtaacg cgattacgat ggggatattt tggcatttga
    26881 tcggcgcggc cagtgcagcc tgtttttacg ctccgttcaa aaaagtaaaa aaatggtcat
    26941 gggaaaccat gtggtcagtt ggtgggattg attcgtggat tattctgccg tgggccatca
    27001 gcgccctgtt actaccgaat ttctgggcgt attacagctc gtttagtctc tctacgctgc
    27061 tgcctgtttt tctgttcggc gctatgtggg ggatcggtaa tatcaactac ggcctgacca
    27121 tgcgttatct cggcatgtcg atgggaattg gcatcgccat tggcattacg ttgattgtcg
    27181 gtacgctgat gacgccaatt atcaacggca atttcgatgt tctgattaat accgaaggcg
    27241 gacgcatgac gttgctcggc gttctggtgg cgctgattgg cgtagggatt gtgactcgcg
    27301 ccgggcagtt gaaagagcgc aagatgggca ttaaagccga agagttcaac ctgaaaaaag
    27361 ggctggtgct ggcggtgatg tgcggcattt tctctgccgg aatgtccttt gcgatgaacg
    27421 ccgcaaaacc gatgcatgaa gccgctgccg cacttggcgt cgatccactg tatgtcgctc
    27481 taccaagcta tgttgtcatc atgggaggcg gcgcgatcat caacctcggt ttctgcttca
    27541 ttcgtctggc aaaagtgaag gatttgtcgc taaaagccga cttttcgctg gcaaaaccgc
    27601 tgatcattca caatgtgtta ctctcagcac tgggcgggtt gatgtggtat ctgcaattct
    27661 ttttctatgc ctggggccac gcgcgtattc cggcgcagta tgactacatc agctggatgc
    27721 tgcatatgag cttctatgta ttgtgcggcg gtatcgtcgg gctggtactg aaagagtgga
    27781 acaatgcagg tcgccgtccg gtaacggtgt tgagcctcgg ttgtgtggtg attattgtcg
    27841 ccgccaacat cgtcggcatg ggcatggcga attaatcttt ctgcgaattg agatgacgcc
    27901 agtggctggg cgtcatcccg gtttcccggg taaacaccac cgaaaaatag ttactatctt
    27961 caaagccaca ttcggttgaa atatcactga ttaacaggcg gctatgctgg agaagatatt
    28021 gcgcatgaca cactctgatc tgccgcagat attgattgat ggtcatgcca gtctgctggc
    28081 gaaattgctg acgcaaaacg cgctcactgc acaatgcctc atcacaaaat ttatccagcg
    28141 caaagggact tttcaggctg gccgccagcc gggtaatcag cttatccagc aatgtttcgc
    28201 tggatgttgg cggcaacgaa tcgctggtgt aacgatggcg attcagcaac atcaccaact
    28261 gcccgaacag caactctgcc atttcgttag caaacggcac atgctgacta ctttcatgct
    28321 caagttgacc gataacctgc cgcgcctgcg ccatccccac gctacctaag cgccagtgtg
    28381 gttgccctgc gctggcacta aatcccggaa tcgccccctg ccagtcaaga ttcagcttca
    28441 gccgctccgg gcaataaata atattctgca aaaccagatc gttaacggaa gcgtaggagt
    28501 gtttatcgtc agcatgaatg taaaagagat cgccacgggt aatgcgataa gggcgatcgt
    28561 tgagtacatg cagaccattg ccgcgccaga caattaccag ctcacaaaaa tcatgtgtat
    28621 gttcagcaaa gacatcttgc ggataacggt cagccacagc gactgcctgc tggtcgctgg
    28681 caaaaaaatc atctttgaga agttttatct gatgcgccac cgtggctacc tcggccagag
    28741 aacgaagttg attattcgca atatggcgta caaatacggt gagtagattc gcgttattgc
    28801 aaaaagccat cccgtccctg acgaatatca cgcggtgacc agttaaactc tcggcgaaaa
    28861 agcgtcgaaa agtggttact gtcgctgaat ccgcagcgat aggcgatgtc agtaacgctg
    28921 gcctcgctgt ggcgtagcag atgtcgggct ttcatcagtc gcaggcggtt caggtaacgc
    28981 tgaggcgtca gtcccgtttg ctgcttaagc tgccgatgta gcgtacgcag tgaaagagaa
    29041 aattgatccg ccacggcatc ccaattcacc tcatcggcaa aatggtcctc cagccaggcc
    29101 agaagcaagt tgagacgtga tgcgctgttt tccaggttct cctgcaaact gcttttacgc
    29161 agcaagagca gtaattgcat aaacaagatc tcgcgactgg cggtcgaggg taaatcattt
    29221 tccccttcct gctgttccat ctgtgcaacc agctgtcgca cctgctgcaa tacgctgtgg
    29281 ttaacgcgcc agtgagacgg atactgccca tccagctctt gtggcagcaa ctgattcagc
    29341 ccggcgagaa actgaaatcg atccggcgag cgatacagca cattggtcag acacagatta
    29401 tcggtatgtt catacagatg ccgatcatga tcgcgtacga aacagaccgt gccaccggtg
    29461 atggtatagg gctgcccatt aaacacatga atacccgtgc catgttcgac aatcacaatt
    29521 tcatgaaaat catgatgatg ttcaggaaaa tccgcctgcg ggagccgggg ttctatcgcc
    29581 acggacgcgt taccagacgg aaaaaaatcc acactatgta atacggtcat actggcctcc
    29641 tgatgtcgtc aacacggcga aatagtaatc acgaggtcag gttcttacct taaattttcg
    29701 acggaaaacc acgtaaaaaa agtcgatttt tcaagataca gcgtgaattt tcaggaaatg
    29761 cggtaagcat cacaccacca caattcagcg aattgtgaac atcatcacgt tcatctttcc
    29821 ctggttgcca atgacccatt ttcctgtcag taacgagaag gtcgcgaagt caggcgcttt
    29881 ttagactggt cgtaatgaaa ttcagcagga tcacattatg acctttcgca attgtgtcgc
    29941 cgtcgatctc ggcgcatcca gtggacgcgt gatgctggcg cgttacgagc gtgaatgccg
    30001 cagcctgacg ctgcgcgaaa tccatcgttt taacaatggg ctgcatagcc agaacggtta
    30061 tgtcacctgg gatgtgggta gcctggaaag tgccattcgc cttggattaa acaaggtgtg
    30121 cgaggaaggg attcgtatcg atagcattgg gattgatacc tggggcgtgg actttgtgct
    30181 gctcgaccaa cagggtcagc gtgtgggcct gcccgttgct tatcgcgata gccgcaccaa
    30241 tggcctgatg gcgcaggcac aacaacaact cggcaaacgc aatatttatc aacgtagcgg
    30301 catccagttt ctgcccttca atacgcttta tcagttgcat gcactgacag agcaacaacc
    30361 tgagcttatt ccacacattg ctcacgctct gctgatgccg gattacttca gttatcgcct
    30421 gaccggcgag atgaactggg agtacaccaa cgccacgacc acgcaactgg tcaatatcaa
    30481 tagcgacgac tgggacgagt cgctactggc gtggagcggt gccaacaaag cctggtttgg
    30541 tcgcccgacg catccgggta atgtcatagg tcactggatt tgcccgcagg gtaatgagat
    30601 tccggtggtc gccgttgcca gccatgatac cgccagcgcg gttatcgcct cgccgttaaa
    30661 cggttcacgc gccgcttatc tctcttctgg cacctggtca ttgatgggct tcgaaagcca
    30721 gacgccattt accaatgaca cggcgctggc cgccaatatc accaatgaag gaggggcgga
    30781 aggtcgctat cgggtgctga aaaatattat gggcttatgg ctgcttcagc gggtgcttca
    30841 ggagcggcaa atcaacgatc tcccggcgct tatcgccgcg acacaggcac tgccggcttg
    30901 ccgcttcacc atcaatccca atgacgatcg ctttattaat cctgaggcga tgtgcagcga
    30961 aattcaggct gcgtgtcggg aaacggcgca accaatcccg gaaagtgatg ctgaactggc
    31021 gcgctgtatt ttcgacagtc tggcgttgct gtatgccgat gtgttgcatg agctggcgca
    31081 gctgcgcggt gaagatttct cgcaactgca tattgtcggc ggtggctgcc agaacgcgct
    31141 gctcaaccag ttatgtgctg atgcctgcgg tattcgggtg atcgccgggc ctgttgaagc
    31201 ctcgacgctc ggcaatatcg gcatccagtt aatgacgctg gatgaactca acaatgtgga
    31261 tgatttccgt caggtcgtca gcaccaccgc gaatctgacc acctttaccc ctaatcctga
    31321 cagtgaaatt gcccactatg tggcgcagat tcactctaca cgacagacaa aggagctttg
    31381 cgcatgacca ctcaactgga acaggcctgg gagctggcga aacagcgttt cgcagcggta
    31441 gggattgatg tcgaggaggc gctgcgccaa cttgatcgtt tacccgtgtc aatgcactgc
    31501 tggcaaggcg atgatgtttc cggttttgaa aacccggaag gttcgctgac cggggggatt
    31561 caggccacag gtaattatcc gggtaaagcg cgtaatgcca gcgagctgcg tgccgatctg
    31621 gaacaggcta tgcggctgat tccggggccg aaacggctta atttacatgc catctatctg
    31681 gaatcagaca cgccagtctc gcgcgaccag atcaaaccag agcacttcaa aaactgggtt
    31741 gaatgggcga aagccaatca gctcggtctg gattttaacc cctcctgttt ttcgcatccg
    31801 ctaagcgccg atggctttac gctttcccat gccgacgaca gcattcgcca gttctggatt
    31861 gatcactgca aggccagccg ccgcgtttcg gcctattttg gcgagcaact tggcacgcca
    31921 tcggtgatga acatctggat cccggatggc atgaaagata tcaccgttga ccgtctcgct
    31981 ccgcgccagc gtttgctggc agcactggat gaggtgatca gcgagaagct ggatccggcg
    32041 caccatatcg acgccgttga gagcaaatta tttggtattg gtgcggagag ctacacggtt
    32101 ggctccaatg agttttacat ggggtatgcc accagccgcc agacggcgct gtgcctggac
    32161 gccgggcatt tccacccgac tgaagtgatt tccgacaaga tttccgccgc catgctgtat
    32221 gtgccgcagt tgctactgca cgtcagtcgt ccggttcgct gggacagcga tcacgtggtg
    32281 ctgctggatg atgaaactca ggcgattgcc agtgagattg ttcgtcacga tctgttcgac
    32341 cgtgtgcata tcggtctcga tttctttgat gcctctatca accgcattgc cgcgtgggtc
    32401 attggtacac gcaatatgaa aaaagccctg ctgcgtgcgt tgctggaacc taccgctgag
    32461 ctgcgcaagc tggaagcggc gggcgattac actgcgcgtc tggcactgct ggaagagcag
    32521 aaatcgttgc cgtggcaggc ggtctgggaa atgtattgcc aacgtcacga taccccagcg
    32581 ggtagcgaat ggttgaagag cgtgcgggct tatgagaaag aaattttgag tcgccgcggg
    32641 taaacactgc cggatgcggc gcgagcgcct tattcggcct acgggtcgtg catccgacaa
    32701 caccgaattt acaggaacac agaacatgca aaacattacc cagtcctggt ttgtccaggg
    32761 aatgatcaaa gccaccaccg acgcctggct aaaaggctgg gacgagcgca atggtggtaa
    32821 cctgacactg cgcctggatg acgccgatat cgcaccgtat cacgacaact tccaccaaca
    32881 accgcgctat atcccgctca gccagcccat gcctttactg gcaaatacac cgtttattgt
    32941 caccggctcc ggcaaattct tccgcaacgt ccagcttgat cctgcggcta acttaggcgt
    33001 cgtaaaagtc gacagcgacg gcgcgggcta ccacattctc tgggggctaa ctcacgaagc
    33061 cgttccaacc tctgaactcc cggcgcactt cctctcccac tgcgaacgca ttaaagccac
    33121 caacggtaaa gatcgggtga tcatgcactg ccatgccacc aacctgatcg cccttaccta
    33181 cgtactggaa aacgacaccg cggtcttcac tcgccaactg tgggaaggca gcaccgaatg
    33241 tctggtagtg ttcccggatg gcgtcggcat tttgccgtgg atggtgcccg gcacggacga
    33301 aatcggccag gcgaccgcac aggaaatgca aaaacattcg ctggtgttgt ggcccttcca
    33361 cggcgtcttc ggcagcggtc cgacactgga tgaaacattc ggtttaatcg acaccgcaga
    33421 aaaatcagca caagtattag tgaaggttta ttcgatgggc ggcatgaaac agaccatcag
    33481 tcgtgaagag ctgatagcgc tcggccagcg tttcggcgtt acaccactcg ccagtgcgct
    33541 ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgta atgcaggtta accccgacgc
    33601 ccacgaagag tatcagcgtc ggcataaccc catctggccg gaactggaag cagtgttgaa
    33661 atctcacggt gcgcataact acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc
    33721 cacggtagag attgaatctg aagaacggtg gaatgcggta gccagcactg atgtttgcca
    33781 acgttggtgg aaatatatga ccgacgttat gccagctaac ccggataaca gcccagtcag
    33841 tagcgagctg caagaagtgt tttacctgcc ctaatgagtt gcggtgccgg aaaatatcca
    33901 gcaccgcgcc ttcatcagaa gaagtattta aagcgaacgc gtgcgccgta acggtcatca
    33961 gtatcatcac cgtgtttttt atcaccgtcg ctatccagaa tggagtaata cgtcccgagg
    34021 taaatattaa agttctccat atccatcaca ttagcgaact gataagacgc atgaatggtg
    34081 tgaatggtgt acgtcccttc atcatcaatc cagcagtcgt tatcacaaac tacaccgctg
    34141 aattcatcaa tcttattgtg cgcgtagata taacccagct cgaaacgttt ccacagcgcg
    34201 ttaatcccgg cagtgaaatc tttctcatta ttagcatcta aataggcggt attaagatta
    34261 acaacgacgc cattttccgg atccgttttc aggccattcc aggtcatgct cataccatag
    34321 ccggtacgat cggactgatc gacaaaatta cccttgctat cggtataacc ataagcatta
    34381 tttaccacgt tcgcttccat cgctgcggaa acggtaaatt cttccgtcgg cgaccaggca
    34441 attaccggac gcagataagc aacatttttc tgctgctcca catcgcgtcc atgataatta
    34501 ccgtcattat ataaagatgt tccgtcttca agtaacgtgt ttaattcaaa atacaagtta
    34561 tcaagttgtt tgctgacgag gaaattaccg ccagcgttag agcgcccgcg gccctctttc
    34621 atcatataga tataaccgct gccatcgtca taaagatcgt tcgcagtatt accggaatgt
    34681 tcaacaaagg tatcctgatt cagcgggaac atatcgtagg cttcaaaacg gcctactttg
    34741 accttccagt cgttttgttt gccaaagaag aataccgcat catcgatatt tactgaaccg
    34801 tgcatgtccc ccagcggttg tgcggagaac ccggcgaaat agccattatc cagttttcgc
    34861 ataccatcaa aacctaacag aatacgacca ttgagattcc actgttcatt agtgggatcg
    34921 ctattcacat cagcgttggt cattgccagc aggccatgat tactttccgc atccatatta
    34981 aattctacat cgccgtagat ttttaaatcg ccataacctg agagcaaaag attaggtgag
    35041 gtggaatttt tctttgcaga ttccgccgtt gcctgtccat ttaccggcgt attgccctgg
    35101 gcagccttaa tttcgcggat ctcagcggct tgttgtgtct gcagctgttt aacctggctc
    35161 tctgcttttg ctgcccgcat ttctgccgct tccaggcgtt cttccagcaa tgccatgcgt
    35221 tgttccagcg ttaaatgctg tgtttttgcc attccggtac tacatgccaa taataaaccg
    35281 atggctaaag ataatgtcga ttttttcatt atttacgttc cctgaaataa aagagaccct
    35341 acgctttcca aaaggaatac gtatatagag aaatataagg tgtgcacttt cgaattttta
    35401 atccaacttc agcaggccga tggatagtat cccgctgcat aatatgagta aatccgctga
    35461 tttcctggaa cgtgaggaat ataacaatag tgccgttttt aactaaatat cgtaaaaaat
    35521 attagatata attcacgctg actcataagc cacaaatata aataaccttg ctgcaaaaca
    35581 tgtgcgacag ttaaacattc acaacatcat taaataatga tttcttattc actgcattta
    35641 ttttgcccat caatatgtgc cttactccgc aaaatttatc cttcactctg ccttatattg
    35701 tcacgacttc acgacatatc cgcgctcatc acaaaaccgc tccgccgtta caattaacta
    35761 taatattttt tcatggaatc caataatggc agcacttact gcaagctgta ttgacctgaa
    35821 tattcagggc aatggcactt attccgttct gaagcagttg gcgacaatgg cgttacaaaa
    35881 cggttttatc accgactcac accagttcct gcaaactctg ctgctgcgcg aaaaaatgca
    35941 ctccaccggt tttggttccg gcatcgccgt gccgcacggt aaaagcgcct gcgttaaaca
    36001 accgttcgtg ttattcgccc gcaaagcgca ggccattgac tggaaagcca gcgacggcga
    36061 agacgtcaat tgctggatct gcctcggcgt gccgcaaagt ggcgaagagg atcaggtcaa
    36121 aatcatcggc acactgtgtc gcaaaattat ccaccaggat tttatcaacc aactgcgaca
    36181 gggcgacgcc gaccaggtgc ttgctttgtt aaatcaaacc ctcagctcat aaggaagtgg
    36241 cgatggagtc atccttacgt attgtcgcga tcaccaactg ccccgccggg atcgctcaca
    36301 cctacatggt ggcggaagcc ctggaacaga aagcccgttc tctcggtcat accattaaag
    36361 ttgaaactca aggatcaagc ggcatcgaaa accgcttatc cagcgaagag attgccgctg
    36421 ccgattacgt gattctcgct accgggcgtg gcctgagcgg tgatgatcgc gcgcgatttg
    36481 ccgggaaaaa ggtttatgaa attgccatct cccaggcgct gaaaaatatc gaccatattt
    36541 tcagtgaatt accgacaaac tcacaatttt ttgccgcaga tagcggcgtg aagctcggca
    36601 aacaggaagt gcaaagtggc agcgtaatga gccacctgat ggcgggcgtc tctgcggcgc
    36661 tgccgtttgt catcggtggc ggtattctgg tggcgcttgc caacatgctg gtgcagttcg
    36721 gtttgccgta taccgatatg tcgaaaggcg cgccgtcgtt tacctgggtg gttgaatcca
    36781 tcggttatct cggcttcacc tttatgatcc ccatcatggg cgcttatatt gcctcgtcga
    36841 ttgccgataa acccgctttc gctccggcgt ttctggtttg ctatctggcg aacgacaaag
    36901 ccctgctcgg cacccagtct ggcgcgggct tcctcggtgc ggtggtgctt gggctggcga
    36961 ttggctattt cgtcttctgg ttccgtaaag tgcgccttgg caaagcatta caaccgctgc
    37021 tcggttccat gctgatcccg ttcgttactc tgctggtttt tggcgtactg acttactacg
    37081 ttatcggacc ggtgatgtcc gacctcatgg gcgggttgct gcacttcctg aacaccattc
    37141 ctccgtcaat gaagtttgcg gcggcgtttc tggtcggtgc aatgctggcg ttcgatatgg
    37201 gcggccctat caataaaacc gcctggttct tctgcttctc actgctggaa aaacacattt
    37261 acgactggta cgccatcgtc ggcgttgtcg cgctgatgcc gcctgtcgcg gcgggtcttg
    37321 cgacgttcat tgcgcccaag ctgtttactc aacaggaaaa agaagccgcc agtagcgcca
    37381 ttgtggtcgg tgccacggtc gcgaccgaac cggctattcc ttacgctctg gcggcaccac
    37441 tgccaatgat taccgccaat acgctggcgg gcggcattac cggcgtgctg gtgatcgctt
    37501 tcggaatcaa acgactggca cctggtttgg gtatctttga cccgctgatc ggcctgatgt
    37561 cgccggtggg ttcgttttat ctggtgctgg cgattggtct cgcgctgaac atttcattca
    37621 ttatcgtcct gaaaggatta tggttacgcc gcaaagcgaa agccgcgcaa caggagcttg
    37681 cccatgaaca ttgagttact gcaacagttg tgcgaagcca gcgccgttag cggcgatgaa
    37741 caggaagttc gcgacattct gatcaacacg ctggaacctt gcgttaatga gatcaccttt
    37801 gatggtctgg gcagctttgt tgcccgtaag gggaataaag gtccaaaagt tgccgttgtc
    37861 gggcatatgg atgaagtcgg ctttatggtc acccacatcg acgagagcgg ttttctgcgt
    37921 tttaccacca ttggcggctg gtggaatcag tcgatactca accaccgggt aaccatacgc
    37981 acacacaagg gatttaaaat ccctggtgtg attggttccg tcgcgcctca tgcgttaacg
    38041 gaaaagcaaa agcaacaacc gctgtcattt gatgagatgt tcattgatat tggcgcgaac
    38101 agtcgcgaag aagcggaaaa acgcggcgtg gaaattggta attttattag cccggaagcc
    38161 aattttgcct gctggggcga agataaagtg gtcggcaagg cgttggataa ccgcatcggc
    38221 tgcgcaatga tggctgagct attgcagacg gtgaataatc ccgaaattac gctgtatggc
    38281 gttggcagtg tggaagaaga agttggcctg cgcggggcgc aaacctcggc tgaacacatt
    38341 aaaccggatg ttgtgatcgt gctggatacc gccgtcgcag gcgatgttcc gggcattgat
    38401 aacattaaat acccgctaaa actgggccag gggccggggc tgatgctgtt tgataaacgc
    38461 tacttcccca accagaagct ggtggtggcg ttaaaaagct gtgccacaca taacgattta
    38521 ccgctgcaat tttccaccat gaaaaccggt gcgacggatg gcgggcgcta caacgtgatg
    38581 ggcggcgggc gtccggttgt cgcgctgtgc ctgccaacgc gttatctgca cgccaacagc
    38641 ggcatgattt caaaagccga ttacgatgct ctgctcacgc tgatacgggg ttttctgacg
    38701 accttaactg cggagaaaat caacgcgttt agccagttcc gtcaggtgga ttaatgctaa
    38761 acgaacgcca gttaaagatt gtcgatctgc tggagcaaca gccgcgcacg cctggcgagc
    38821 tggcacaaca gactggcgtt tcgggcagga ccatcctgcg cgacattgac tatctcaact
    38881 ttacccttaa cggcaaagcc cgcatttccg ccagtggcaa cgcgggctat cagctggaaa
    38941 tcttcgagcg ccgcagcttt tttcagttgc tgcaaaagca cgataacgac gatcggctgc
    39001 tggcgctgct attactgaat accttcactc cccgtgcgca actcgcctcg gcgcttaatt
    39061 tgccagaaac gtgggtagca gagcgcctgc cccgcctgaa acagcgctat gaacgcggtt
    39121 tttgtctcgc tagtcgccct ggtttgggcc atttcattga tgagacagaa gagaaacgca
    39181 ttattttgct ggcgaatctt ttacgcaaag atccgttttt aattcctctg gcgggcgtaa
    39241 cacgagacaa ccttcagcaa ttatccacgg tctgcgacaa ccaacaccgc tggccgctga
    39301 tgcagggtga ttatctctcc agcctgattc tggcgattta cgccctgcgt aatcaactga
    39361 ccgatgagtg gccgcaatat cccggtagcg agataaaaca gatcgttgaa tatagcggcc
    39421 tgtttcttgg tgataacgct gtaagaacgt tgacggggtt gatagaaaaa cagcatcaac
    39481 aggcgcaggt tatttgtgtc gataatgtgc tggcattgct gcaaagggtg ccgggcattg
    39541 cgtcattgaa tattattgat acgcaactgg ttgagaatat taccgggcat ttattacgtt
    39601 gccttgccgc accagtatgg attgctgagc accgccaaag cagcatgaat aacctgaaag
    39661 ccgcctggcc tgcggccttt gatatgagtc tgcactttat tacgctactg cgtgaacagc
    39721 tcgatattcc ccttttcgac agcgatctga tcggtttgta ttttgcctgt gcgctggaac
    39781 gacatcaaaa cgaacgccag ccgatcattt tgctctcgga ccagaacgcg attgccacta
    39841 ttaatcagct cgccattgag cgcgatgtcc tgaattgtcg ggtgattatt gcccgcagcc
    39901 tgagcgatat taccgccatt agccaggaga ttacgccgtt attgatcgtc aataacagcc
    39961 actatttgct ggatgacgcg gtaaaaaata gtattaccat caaaaatatc attaccgccg
    40021 ccgggattga gcaaattaaa cattttcttg ccacggcatt tattcgccag cagccggagc
    40081 gttttttctc tgcccccgga agttttcatt tttcgaatgt acgcggtgaa aactggcaac
    40141 atattaccca gcaaatatgt gcacaattag tgacgcaaca ccacattacc gccgatgaag
    40201 cacaacggat catcgcccgc gaaggcgaag gtgaaaacct gattgttaat cgtctcgcca
    40261 tcccacattg ctggagcgaa caggaacgcc gttttcgtgg attttttatt accctcgccc
    40321 aaccggttga ggtgaataac gaagtagtta accatgtgct gatcgcctgt gccgccgccg
    40381 atgcgcgtca tgagctaaaa atatttagct atctggcaag cgtattgtgt cagcatccgg
    40441 cagaggttat tgccgggtta aaaggatatg aggcatttat ggagttactt cacaaggggt
    40501 gaactgtgtc ggatgcgccg catccgacac tgcatttatc accaaaatca aaaacgttta
    40561 acgcaggcta ttatagtcat cgaccatttc gttgtactcg cgtaacgctc gcggaaatga
    40621 gtcgtcgacc atccagctgg tgtttgcatc ctgaagttgc tctttatccc agtcagagta
    40681 cggaacttta tctctgacgc ggcgaacata ttttttagcc tcgagttgat actggtctgc
    40741 cgaattaatg aacgagaaat tcataccgcc tttatccgct tctttggcct gcgccaccag
    40801 cgtttccagt tcagagactt tcttcattgc cgcatcaaca tcaaacttct cttgcgaaat
    40861 caggttatta atttgtttcg ctgaaatcat gactgccaga gagtagtagt ggaatgtttt
    40921 cccttctctt tgttcaatat ttttcagttc cgcaagctgg cgcttatcgt taatttcctg
    40981 aatcgccgca tgataagatt ctgctacagg ttcaaaggct tcaagatttt ttaagaatgt
    41041 ctggtgcagc gttttccctt tcgcaaacgc atcgtcttta taattttcct gggtgtaata
    41101 tttatccatt tcgttaatgg tattacccaa cgccacggca gcatcaatat aactcaccgc
    41161 cacgccatca ataggttgca gcgcaggcgt taacgccact acacgcattt tgtttataaa
    41221 taaaataata aaattcacga aatgaataag tcctgcaatt taatatatat cgcaggactt
    41281 attttaatta attgctcaaa cgctgcggat gggtataaac cgttgcccga cccggtttac
    41341 aaaagcccac cagcgtcaaa ttacagcgtt cagccacttc tacagcaagt gtggtcgcgg
    41401 cagacaccgc aaacaaaatc tctacgccac acatcgccga cttttgcacc atttcataac
    41461 tggcacgact ggaaaccagc accgcacctt gctgccagct ttccccttct tgtgaccggc
    41521 ggcctaacag tttgtccagc gccacatggc gacccacgtc ttcatgcccg ccgaccagtt
    41581 cgccagatgg caacatccat gcagcggcgt gagtacaacc agtcagttgc cccactggct
    41641 ggaaatcatt gagatgacgt aatgcatcat ccagtttgtt gagatcaaac gtctgggtga
    41701 acggcagcgg ctgcaccggt ttcccgatat cgttaagctg ttctacaccg catacgccac
    41761 agcccgtacg tcccgccagc gcccggcggc gttccttcaa ccccataaag cggcggctgg
    41821 aaagctcaat ttgaacttca agaccattac aggaaggaac gacatccatg ccaaagatat
    41881 cgcgcggact ttcgattatc ccctcggaaa gcgaaaaacc gagcgcaaag tactcaaggt
    41941 ctttgggcga cgccatcatc accacatgcg aaatgccgtt gtagaccagc gcaacgggaa
    42001 cttcttccgc gacctcatct aaccgtgggt gttgtaaatc atcacgccgc cataactcaa
    42061 tttgacgaac acctgtgata tttgtcacat tttcgatttc tttttgctgt gtttttttca
    42121 ctttctctta accagataag aacacacaca ccaacattat ggtattctgt tacaaaccct
    42181 tcctggatgg agggaaattg agccaattct ggacctttgc ggccccttcc gcaaagaaaa
    42241 ataactccca ctccctgcac acgcagcaag cgaatgtaaa tgggacgtga caatgtcgaa
    42301 acaaggagca atccatgcag gtcagcagaa ggcagttctt taagatctgc gctggcggta
    42361 tggcaggcac cacggcggcg gcactgggtt ttgcccccag cgtagcactc gcggaaaccc
    42421 ggcagtataa actgctgcgc acccgcgaaa cccgtaatac ctgcacctat tgttccgtag
    42481 gctgtgggct gttgatgtac agcctcggtg acggagcaaa aaacgccaaa gcatctatct
    42541 tccatatcga aggcgatccg gatcacccgg tcaaccgcgg tgcactttgt ccgaaaggcg
    42601 ctggcctggt ggatttcatc cactccgaaa gccgtctgaa gtttccggaa taccgtgcgc
    42661 caggttctga taaatggcag caaatcagtt gggaagaggc gtttgatcgc atcgccaaac
    42721 tgatgaaaga agaccgcgat gctaactaca ttgcgcaaaa cgccgaaggc gtgaccgtta
    42781 accgctggct ctccaccggg atgctgtgtg cttccgcgtc gagtaacgaa accggctatt
    42841 taacgcaaaa attctcccgc gcgctgggta tgctcgcggt cgacaaccag gcgcgtgtct
    42901 gacacggacc aacggtagca agtcttgctc caacatttgg tcgcggtgcg atgaccaacc
    42961 actgggtcga catcaagaac gccaacctcg tcgtggtgat gggcggtaac gccgctgaag
    43021 ctcacccggt cgggttccgc tgggcgatgg aagccaaaat tcacaacggc gcgaagctga
    43081 ttgtgatcga tcctcgcttt acgcgtacgg cggcggtggc tgactactat gcccctattc
    43141 gttccggtac tgacattgct ttcctgtcag gcgtattgct gtacctgctg aacaatgaaa
    43201 aattcaaccg cgaatacacc gaagcctata ccaacgccag cctgatcgtg cgtgaggatt
    43261 acggctttga agatggcctg ttcaccggct atgacgcgga aaaacgcaag tacgataaat
    43321 cctcctggac ttacgaactg gacgaaaacg gcttcgccaa acgcgatacc acgctgcaac
    43381 atccgcgctg cgtgtggaac ttgctgaaac agcacgtttc ccgctacacg ccagatgtgg
    43441 ttgaaaacat ctgtggtacg ccaaaagacg cgttcctgaa agtctgcgaa tacatcgcag
    43501 aaaccagtgc tcacgataaa actgcctcgt tcctgtatgc cctcggctgg acgcaacact
    43561 ccgttggtgc gcaaaacatt cgtacgatgg cgatgatcca gctgctgctc ggcaacatgg
    43621 ggatggcagg cggcggcgtt aacgccctgc gcggtcactc taatattcag gggctgacgg
    43681 acctggggct gctgtcgcag agcctgccag gttacatgac gctgccaagc gagaagcaga
    43741 ccgatctgca aacctacctt accgccaaca cgccaaaacc gctgctggaa ggccaggtaa
    43801 actactgggg caactacccg aaattcttcg tctctatgat gaaggccttc tttggtgata
    43861 aagcgacggc ggaaaatagc tggggctttg actggttgcc gaagtgggat aaaggctacg
    43921 acgtcctgca atacttcgag atgatgaaag aaggcaaggt caatggctat atctgccagg
    43981 gctttaaccc tgttgcctca ttcccgaaca aaaacaaagt gatcagttgt ctgtcgaaac
    44041 tgaagttcct cgtcactatc gacccgctga acactgaaac ctctaacttc tggcagaacc
    44101 acggtgagct gaacgaagtt gactcgtcga agatccagac cgaagtgttc cgtctgccat
    44161 cgacctgctt cgcggaagag aacggttcaa tcgttaactc aggtcgctgg ttgcagtggc
    44221 actggaaagg tgcggacgcc cctgggattg cgctgactga cggcgagatc ctctccggta
    44281 tcttcctgcg cttgcgcaag atgtatgccg aacagggtgg cgcgaacccg gaccaggtgc
    44341 tgaacatgac ctggaactac gccatcccgc atgagccgaa gtcggaagaa gtggcgatgg
    44401 agagcaacgg taaggcgctg gccgatatta ccgatccggc aaccggtgcg gttatcgtca
    44461 agaaaggcca acaacttagc tcgttcgccc aactgcgcga tgacggtacc acctcctgtg
    44521 gctgctggat tttcgccggt agctggacgc cagagggcaa ccagatggcg cgtcgtgata
    44581 acgccgatcc gtctggcctc ggtaacacgc tgggctgggc atgggcatgg ccgcttaacc
    44641 gccgcattct gtataaccgc gcctccgcag atccgcaggg taacccgtgg gatccgaagc
    44701 gtcagttgct gaaatgggac ggcactaagt ggaccggctg ggatattccg gactacagcg
    44761 cagcgcctcc gggcagtggc gttgggccgt ttatcatgca gcaggaaggc atggggcgtc
    44821 tgtttgccct cgataagatg gcggaaggtc cgttcccgga acactacgag ccgtttgaaa
    44881 cgccgctggg aactaacccg ctgcatccaa acgttatctc gaacccggct gcacgtatct
    44941 ttaaagacga cgccgaagca ttgggtaaag ccgataagtt cccgtatgtc ggaaccacct
    45001 atcgtctgac cgagcacttc cactactgga ccaaacacgc gctgttgaac gcgattttgc
    45061 aaccagagca gtttgtggaa atcggtgagt cgctggcgaa taaacttggc attgcccagg
    45121 gcgatacagt gaaagtctcc tccaaccgtg gctatataaa agccaaagcg gtggtgacca
    45181 aacgtattcg cacgctgaaa gcgaacggca aagatatcga taccatcggt atcccgattc
    45241 actggggcta tgaaggtgtt gcgaaaaaag gctttattgc caatacgtta acgccattcg
    45301 ttggtgatgc gaacacgcag acgccagagt ttaagtcctt ccttgtgaat gtggaaaagg
    45361 tgtaacggag acgacatatg gcttatcaat ctcaagatat cattcgtcgt tccgcgacta
    45421 acggtctgac ccccgcgcct caggcgcggg acttccagga agaagtggcg aaactcatcg
    45481 acgtcaccac ctgtatcggc tgtaaagcct gtcaggtggc gtgttcagag tggaacgaca
    45541 ttcgcgatac cgtcggcaat aacattgggg tgtacgacaa ccccaatgat ttaagtgcca
    45601 aatcgtggac ggtgatgcgc ttctcggaag tggaacagaa cgacaaactg gaatggctga
    45661 tccgcaaaga cggctgtatg cactgttccg atccaggctg cctgaaagcg tgcccggcgg
    45721 aaggagcaat tattcagtat gccaacggta tcgtcgactt ccagtccgag cagtgcatcg
    45781 gctgcggtta ttgcattgcg ggctgtccgt tcgacattcc gcgcctcaac ccggaagaca
    45841 accgcgtcta caaatgtacg ctgtgcgttg accgcgtggt ggttgggcaa gaaccggcct
    45901 gcgtgaagac ctgcccaacg ggcgcgattc acttcggtac gaaagagtcg atgaaaacgc
    45961 tggcgagcga gcgcgtggcg gagctgaaaa ctcgcggtta cgacaatgcg ggtctgtacg
    46021 atccggcagg cgttggtggt acacacgtta tgtacgtgct gcaccatgct gacaagccaa
    46081 atctgtatca tggcttgccg gagaacccgg aaatcagcga aaccgtgaaa ttctggaaag
    46141 gcatctggaa accgctcgca gctgttggct ttgcggctac cttcgcagcc agtatcttcc
    46201 actacgtggg tgtcggtccg aaccgtgcgg atgaggaaga gaataatctg cacgaagaga
    46261 aagacgagga gcgcaaatga aacgacgtga caccatcgtg cgctacaccg cgccggaacg
    46321 tatcaaccac tggatcaccg ccttctgctt catcctggcg gcggtgagcg ggctgggctt
    46381 tttgttcccg tccttcaact ggttgatgca aatcatgggc acaccgcagc tggcgcgaat
    46441 tctgcacccg tttgtcggcg tggttatgtt tgcctcgttc atcatcatgt ttttccgtta
    46501 ctggcatcac aacctaatca atcgggatga tatcttttgg gcgaagaata ttcgtaagat
    46561 cgtcgtcaac gaggaagtag gtgacaccgg gcgttataac ttcggtcaga aatgcgtttt
    46621 ctgggcggcg attattttcc tggttctgct gctggtgagc ggcgtgatta tctggcgtcc
    46681 ttattttgcg cctgctttct caatcccggt gatccgattc gcgttaatgc tgcattcatt
    46741 tgccgcagtg gcgttaattg tggttatcat ggtgcatatc tacgccgccc tttgggtgaa
    46801 aggcacgatt accgccatgg tggaaggatg ggttaccagc gcctgggcga agaaacatca
    46861 cccgcgctgg taccgtgagg tccgcaagac aacggaaaag aaagctgaat gagtattcgc
    46921 ataatcccgc aagatgagct gggttcgagc gagaaacgta cggcggatat gattccgccg
    46981 ttattgttcc ctcggctcaa gaatttatac aaccgccgcg ccgagcgtct gcgcgagctg
    47041 gcagaaaata atccgctggg tgattacctg cgctttgctg cgcttatcgc ccacgcccag
    47101 gaagtggtgc tgtacgacca tccgctggag atggatctga ccacgcgcat taaagaagcc
    47161 agcgcacaag gcaagccccc gctggatatt cacgttctgc cgcgtgataa gcactggcaa
    47221 aagctgctga tggcgctaat tgctgagctg aaacctgaaa tgagcggccc ggcgctggca
    47281 gtgattgaga atctggagaa ggcatcgact caggagctgg aagatatggc cagcgcactg
    47341 tttgcctctg atttctcgtc cgtcagcagc gataaagcgc cgtttatctg ggctgcgctg
    47401 tcgctctact gggcgcagat ggctaatctg atccccggca aagcccgcgc tgaatacggc
    47461 gaacaacgtc aatattgccc ggtatgtggc tctatgccgg tgtccagcat ggtgcaaatt
    47521 ggcaccactc agggtctgcg ttacctgcac tgcaacctgt gtgaaaccga atggcacgta
    47581 gtgcgcgtaa aatgcagcaa ctgcgagcag agcggcaaac tgcattactg gtcgctggat
    47641 gacgaacagg ccgcgattaa agccgaaagc tgcgatgact gcggcactta cctgaagatt
    47701 ctttatcagg aaaaagaacc gaaagttgaa gccgtggcag atgacctcgc ctctctggta
    47761 ctggacgcgc gaatggagca agaaggctac gcccgcagtt ccatcaaccc gttcctgttc
    47821 ccgggtgaag gggagtaatc tctgacgttt tccgggtggc gctggtcgcc cggattacag
    47881 ctcatagggt gtgacgacac tatctctctt attccgcgtt accacctcaa gctatgctgc
    47941 cagtgcctgt tttgcctcaa ttttcacact gaacaaatcg ataacaatat cgatatcaaa
    48001 tagccaaatc tttttgccat taccattcct cgcgcagctt acgctggtat tcgacaccat
    48061 cttcctcact cccttgccag atgccaaaag cactggctct tgctgtttgc gattgattta
    48121 ccaggtattg atctaccgct tcccgtaata gctccgcacg aggaagatta cgctgcacct
    48181 cgagatcatc aagttgctta aacaactcat tcgataaatt gagtaaaatt ctgcccatat
    48241 ctatacctgc cagagagttc ataaatattt cgccaatatc atttttgaat ctatggagag
    48301 aaaagtaccc ttgccgaatc tttaaagaaa gctcatttac gcatcatttt ttatttttgc
    48361 gaggcgtttt ccagatatga tgtaacagtt gcagttatta taagttcaag taaaacaacc
    48421 ttgtattaat accacaataa gggtatctgg tttttatatt caagaataaa aaaatttttg
    48481 tcattcctta tgctccttac actgatcatc aacaccaact acctccccct tttgcctgcc
    48541 tccctttgct ttacgcaccg ggaattgctg acgttccaga tattgctcta ccgcttcgcg
    48601 taaaattgca gatcgtgaaa ccttacgcca gcccctaagc ttgttcagcc gtttaattgc
    48661 ctcttctgat aaatgaagaa aaaccgtatt catcgccatt accaatacct tacatatatt
    48721 actcattaat gtatgtgcga aaattacgct gaaaaatcat ttaaaaaaag cggatttttg
    48781 catgctgttt tgtttttgcg agacggcatc caaagataat gtaacagttg cagttatttc
    48841 aattgttact caaaaatggt ttagtaataa cccaggatgg tttattactt ttaattaagc
    48901 ggggacacat ctacagaaaa gaaacaacaa ataataagga tgaattatgg gtgctataaa
    48961 tattttatat tcaagaatat ttttttgatt gggtgttcat ttgcttcatc aggatggtgt
    49021 acccgaaaaa gtcagcagct ttaccgcacc agggtttaag gtgaaatcta ccattcataa
    49081 ctgaccggtc agtgccgcac caaccggtct ttaattgtta gtagtagtaa atttgtggat
    49141 aacagtccgg gaaccagacg attccttctg gatcatcccc ctgcattacc cgatgacatt
    49201 ggcgtcgatc atctgcgcga cggcgaagcg tagtatagag ccgcttccat tcatgatcgc
    49261 ccggtgtact gtagtttacc caacaatcca ttgaagggtg acgccattta cctgtaagtg
    49321 ttgggatccc tgaatgatta tgcagcttcg ctttaccatt acgatcccta tctttacgcg
    49381 tattaagcgt ggacattttt agtctcctgt taagagttct aaaaatgtgc ttccttaaaa
    49441 tagttcatca ttttccctta attcgcatag cgtgcttttg cacgaatttc cgatacgcgg
    49501 ggaccattat gatagcgtcg atgcccacac atccaacaag aacaaataca gggagtacga
    49561 aagcaaatac ctgatgcttt tttgcttcca tcgcctgcag tgcgataacg gcagcggatt
    49621 tttttaatcg cgccatatga tggcgacgtt cagcacgatt acgggtcata gtaaaactcc
    49681 tgagcaggcg gcactatgcc gctttcatag gagttctgaa tgctggtgct ttctgtatgg
    49741 cataaccatg cctctaacgg ttaagtgcct gcagcacatg ttgcggatca ttggcaatcg
    49801 ctcgcagcaa agcctttgca ggcccagtcg gtgaacgtcg cccctgctcc cagttacgca
    49861 acgtgtctac gcttacggaa ataagttcgg caaatttaga ttgtgacaac cctgaagcgc
    49921 ggcgtatttc tttgattttc atagcatcaa cttcaaaagt ccgtgaaggg tgtcgcgttc
    49981 cttcaataat ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa agtgccttat
    50041 ccatttacca cctcgtattt aacaaccgga gcattgcttt ttcctgggga gacagatcat
    50101 ctttaatacc cttacgataa ataagcagta atctgatctc ataatgttta acctgatgaa
    50161 agtaaataac cctgacacca gcacgtttcc cctttcctcc tgacacccat cgaacctttc
    50221 ttaacccacc agtttcaggt attacctcac catagtcagg attcaaggcc aggaaaaact
    50281 gaaaacgact gaattcatcg tcggtcagca gtttttgaac atcttcagta aaaatttccg
    50341 tttcgatgaa gagcataatt atacgccatt ggcttagagt aacaatcctt tttgtacgtc
    50401 attgacgtac ccttgaaacc actagcttac tgatagtgct ccagcaaaac cacgtctctg
    50461 ccgggtttgt ctttacattt gcgaggcgct ttccagattt ttcacgcccg tgaatttccc
    50521 tgtatccatc cagcaaaaac gggtgtataa aactgtacat tcatacagca aaatgagttc
    50581 tactatggca ctggaaaaag gaattgcaga gttagtcgaa gagtttatcg ctgcgggtcg
    50641 cccctcatcg cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg ccagcgctgt
    50701 ccttgcgggt gagacagaaa cgcgggtaga tattcagact atcgaacttg aagggatgat
    50761 cttacgaata gtgtcaccgc tgaatgcccc gacattactc ccgacgataa tttattacta
    50821 cggtggctgt tttgttagcg gcgggttcgc aactcacgac aaccagctgc gccagttagc
    50881 ctattacggg caatatcggg ttattgccgt ccagtatcgg ctggcaccgg aacagacttt
    50941 ccccgccgcc catgatgatg cgcaacgagg tgcagaatta gtctggcaac acgccgaacg
    51001 actcggcgtt gataaacaac ggataactct tgccggtgac agtgcgggcg gacatctggc
    51061 gctggtaact gctttgcgat taaagcgtgc tggcgagtgg caaccggcac aactcatctt
    51121 aatttatccc atgcttgatg cgactgcgca tttcgaaagc tacatacgca atggtcacga
    51181 ttacatcatc acacgcgaca ctttactgag cggctttgag atgtatctgc caggcattga
    51241 acgcaggcat cctgaagcca gccctctttg gcgcgatgat ttcaatggct tgccacctgt
    51301 acatatcatt accgctgaat acgacccact ttgcgatgaa ggtgaagcgt tgtatcaccg
    51361 catgacaggg caaggagtgc aatgcacctg ccaacgctac cttggcgtta tccacggatt
    51421 ttttcagtta ggtggtatca gtgaggctgc gcgatcggcg ctgcgggatg tggcctggag
    51481 agcgggaagg taactcctga gcagcggcac tatgccgctt tcacaggagt tctgaatgct
    51541 ggtgctttct gtatggcttc gccatgcccc tcgcggctaa gcgcctgcac acatctctct
    51601 ttgaatgtca tttttgcaat gcattggccg aattctggta attctaatga gttttactct
    51661 tcagactccc atccctacgg tatacgagtt agattcatga atgaaatcga catttttaaa
    51721 aaacgaatct tgtaaaacat ccgtgttgat attaccttgc aacccaacat taaatttgtt
    51781 agagagatga agaactcgaa tatcaaaaag gtcttttaac atcattaatt ctttggctgt
    51841 taatgaatcg caactaaaat atccctgttg atcaaaaagc ttttctattt ctctaccatc
    51901 ggcgaggcgt ttttcaagga gtgtttgcaa catcgagttg ccaaggacaa tggatatcgt
    51961 acgatctttc gcagatttac accccatcac aacggcaaca ttcgtatccc cctcaggagt
    52021 ttcatttatc gccgtcgcta aaatggtggt caaacacgca aacggaactg tatttctttc
    52081 gtgatttaat ttatctacca gctcattcca tttatcaaaa gccgggcgat atttgttgcg
    52141 cgcagaaata tccaactggt taaacaattc agccatgcga ttttttatca tctcgatatc
    52201 cggcgaacca gaaagacttt gcgcataata cggtcctgat gatgttgcat acattaaagc
    52261 cgatcccacg cctgtcgcca tgatgggcgc agctacggtt ttggcaaggg cgacgctgcg
    52321 atggctatgc gcttcacagg ctaaaggaac atattccact gactgcggta attccttaca
    52381 tgcctccctc atcattgcgg tatgctttac aaacaacgtt ttttcactgc ccaaaaaaac
    52441 tttctgctta attgcactaa gaaaacgtaa atcaaaaatg attgtttttg gtttattatt
    52501 ttttggcaca tttcctatta tcccgctaag aacctttctt gctgcatgga gatttttttg
    52561 cacagcacat aacacaattg acgaagcctt ctctactgcg ttattgattt taattttaaa
    52621 atctgcatga tatacgttga cgggcaatgc aagtatatct tcccctacat ctttggctga
    52681 gtatttattt gtgatcgatg gtcgtgaatc cacgagaaaa cttagctgca aattatgctc
    52741 tatattacca gctgtttttg tagtgtgatt aaacatagag atgccacctc tcattctttc
    52801 acacataccc tcaacttttt cacttcgttc ttgaggcaat ttggctttaa cgttaatttg
    52861 atgattttta cccgtgcatt ccttaactac aagaggaatg ttatctaaag tgccatacag
    52921 gctaataacg tcaccaggat ttttatcatg taagtaagtt tgattaatac tcgaagcatt
    52981 cacaaagagg tcaatattat aactgtttat gacttcacct acatcaaatg tgtagtgctt
    53041 actctggagg ctgccatcct tgtttaattg tatatcaatc aatatatccc cggactttgt
    53101 caccatatct ttgccataaa ccatttgcat agccatttcg aggagatttt tatctaactg
    53161 attgcgggcg ttgagtaagc gactaccaaa taatttatat actgttcccg tcattgccag
    53221 cgctgcactt tctggtactt tgcgtttcca cagtgaggtt tctttatttt taaacaggtc
    53281 atatttatta ttactcgaca acccgagttt aagttcgtta acacaatcgc acagttgctc
    53341 ctcaccttcc attttaaggc gatgtgaaaa atcatttaat agcgtttgct tcggtgattc
    53401 attactcatt agttgttttt ctaaattaac aatagaataa ctttcttgtt taatgagatt
    53461 aatattggca accattacag cctccttccc acggtttaag agcaaacatg ccacataatt
    53521 atttacaaca tccagaagca gtaaatgaat gatactgaca atgacgccag tccattatct
    53581 ttgcataatg tttgttagct atagatttta aaaatattta tgagaaaagt atggcaccag
    53641 agggggtttt gactatcaaa aatcgctcaa acctgaacta agatgatgaa ttaagcatta
    53701 tcagcgataa ttgccactca gctctacaaa taatatttaa atgctcctga acagtgatgt
    53761 agcaagacac gtatggtgcc aacgaagaat aaaaatttat agagttctta ttcaatataa
    53821 ccggaatagt cagagactga agtaaatgac ctgaaagaat gagagaggca gcaccgcccc
    53881 tctccatcag ctactcttct tcgttcccgc cctcttcata cgggccaaat ggcttcgcgg
    53941 gcagaacgat atacgtgcct tcaaacactg cacccggcgt ctcgtcgcca aagatttcga
    54001 cctgcatttg tactcgcgct ttgcgcccgc gcgccagacg gtcgagatcg ccgcttaagg
    54061 caccgaggtc ggctaccgca tgaggtttac cgctaatcgg cttgctgtag cgaatatgcg
    54121 catccgccag aataatcgtt ccgcccagat ggcgttcacg cagcatcagc cagataagcc
    54181 cccaaccggt gagcgttgcc agtgagaata aactcccggc aaacagcgta tggtgcggat
    54241 tctgattacc ggtttccggc atggtggtga taaatttttg cccggtatat tgctgaatgc
    54301 gcacgcccat tttttcactc agcgggatat gttcgtacca cgcctgttgc agctgcgcgc
    54361 accagtcgcc gcgatgcaga atgtcatcca gagtggcgac aggttttatc atcaaaaaat
    54421 ggcgaatcgg cgtggtggtt ggcgtggtga tctccccttg attgacaaac cccagcttgg
    54481 cgaaaaactc caccgcgtct tcgcgggcgc tacaggtcac gcgcttaaca ccttcctgac
    54541 gcgccacaga ttcgagggtc atcgccatca gcgttcctaa ccctttgtcc tgcacgtcgg
    54601 gatgaacggc cataaagcga atggacgctt cattgtcggc attaatatac agtcggccta
    54661 ccgccaccag attaccctgc tcgtcgacga ccatctggtg atgcgccatc gcatcccacg
    54721 cgtcgcgttc cgaacctttc ggttgatgca ggggcttacg caacatttcc cagcgaaact
    54781 gatagtaacg ctctaattct tcttctgttt gtggaacccg aaggtgatac atagcgatac
    54841 tctctcttgt ttcccgtgac aaccctggaa gctggctcat acctgcaacc agaatgtcac
    54901 ggggccatca ttgaccagcg atacctgcat atcggcagcg aagcgtcctg tttgcgtgtt
    54961 catctcttgc tgacggcagc gttcgacgaa atagtcatat aacgcctctg cgcgatccgg
    55021 tgatgcacct ttggagaaac ttggacgcat cccccgttcg gtatctgcgg cgagggtaaa
    55081 ctgggaaacc accagtacac tgccgcccgc ctgttgcacg ttgaggttca tcttgccttc
    55141 ggcatcgcta aagatgcgat agccgagcac acgctcgcac agacggtttg ctttctgttc
    55201 gtcgtcatcc ttttcgacac ccaataacac caaaagtccc gcgccaattt cgcccgtcac
    55261 ttctccctcc acggtgacgc tggcacgggt tacgcgttga attaatgcaa tcatggttcg
    55321 tcgtcttctt cttgttcagc tgcttgtttt agtttgcggt attccccgag agtgacagta
    55381 atttccgcac caagcaagac gatacaccat gtccagtaga cccaaacaaa gagaatgggg
    55441 atcaccgcca gcacaccgta aatgagctga tatgagggga acatggtgat ataaagcgcg
    55501 aaacctttct ttcctgcttc gaacaggagt gcggcgacaa acgcgccgac aatcgcgtcg
    55561 cgattaggta cgcggatagt aggaacaatg ctgtacagca accagaagga gatccacgac
    55621 aacagcagcg gaaaaatacg cagcacgttg tcgatgacag tattgagatc gctcgcccag
    55681 cgcagggaga gcaaatagga actgatcgcc agactggccc ctgccagcag cggccccagc
    55741 gttaaaatca tccagtacac ggcaaacgag taaatttttg gtcgcgctcg tttactgcgc
    55801 cagatggtat tcaacgcgct atcgatggag tacatcaaca ataacgcggt gacgatcagc
    55861 ccgcacgccc caacggcggt catcttgttg gaattggcaa caaattgttc gatataccgc
    55921 tgaataacat cgccagtagc aggcagaaag ttggcaaaaa taaagtgacg caactgcatg
    55981 ctgacgtcgg aaaacatggg aaaagcggcg aaaagcgcaa aaacaacggc aacaagcggc
    56041 actaatgaga gcaacgaaac ataggcaagg ttacctgcca gggttgtcat gttgtcctca
    56101 tcaatgcgtt gccagagtag ttttagccag gcccatagtg gacgggtacg gtgcctggct
    56161 ttgtcctgaa tggtttttag cataacacct tcgcgaaata gtccgggatg gtggttttat
    56221 ctttcaccag aatactggta atgcctagct gattcgctcc ttctatatta tcggcgttat
    56281 cgtcgaaaaa gaccgtatcg ctgggtgaaa aaccttccgc ctgcaaaaca tgctgataaa
    56341 ttcgtgcttc aggtttgcgc atccccagat cttgcgacag atagatatgg tcagcagcat
    56401 cacggatttc cgggtattcc tccggccaga aggtagtatg caggcggtta gtattggaaa
    56461 gcaccaccac gcgatgcccc tgctcacgca gtttatgcat gatggcgatc acttccgggc
    56521 gcagcgcaac aaacaccgcc tgccagccgt gggagaactg ctcatagctt agcggcaggg
    56581 ccatctcatg acacagcgcc tctgcgaacg cttcgtcgct aatttcccca cgctcatgct
    56641 gatgaaacgc ctcgcccatg tggaaactct tcttaagcgt agccagtgga acacgcgtta
    56701 aatcgctcca ggctcccagc acacggttaa agtcgatatc gacaatcaca ttacctaaat
    56761 caaagatata gagcattttt gcctcctttc gcgccacaag aaattaactg tagcgggaaa
    56821 ggatgggttt ggctatgtgg tcaggtatga agcaaggtca acggtcacaa aattctacgc
    56881 gtcttcctgg ggaaggatga tttcaatttg gctgtcttgt aagcgtgatt ttagcgcagc
    56941 gggcggcagc tcgtcggtaa agagcgcggt aacctgtgcc acgttaccaa tttcaaccgc
    57001 cgccgaagca tgatacttag tgtgatcggc gaccagcagg atatttctcg cgtgcgccat
    57061 catcgttttc accacgttag cttcgttaac atcaaactcc atcaacgcgc catcgctctc
    57121 aatcgcccca acgcttgtta ccagataatc agcacgaaaa tcagccacaa aggacgccgc
    57181 tgaagggcca atgatcccgc tattatgaga gcgcaacgta ccgccgggca ccatcacttc
    57241 aaagcgcggg ttgtggtaaa gaatatgcgc cacacgcagg ctgttggtga ttatccgcaa
    57301 atgattgtgg ttaagtaacg cccgggcaac atgttcaaca gtcgtaccga tggtgataaa
    57361 tattgtcgat ccatcaggaa tatagtctgc caccgcttcg gcaatcgctt ttttttcctc
    57421 ggtttgcgaa acctcacgct gctcgaacgc cgtattaacg acgctggaag cccgacccgc
    57481 gccaccgtga tggcgcgtaa tcagtccttg ctcgcttaat ttacgaatat cccggcggac
    57541 cgtctgcgtg gagacatcca gcagatttgc cagctcatca atattcatat acccacgctc
    57601 ggcgatcagc atgaggagtt ggtcgtgccg cgggttaccg gttagttcgg taaggctcat
    57661 gaaaattcct cgaaaaacca tcacccgggc attttataca aaaagtgaca aaaaagatcg
    57721 ggtttgatca cagtcaggga tcccggctcg tccaccggga cgcgtgcatt ttaacgccgc
    57781 cacaccgctg gcgaagcgaa ctgactccgc taaattccca ccggttgcca atgccaccgc
    57841 cagcgcaccg tgaaaaacat cacccgcacc ggtggtatct accacatcaa ctttgaaagc
    57901 cggttgatgt tgacgcccgc cattttccag ccagtcgcag ccggcactcc cctgggtcac
    57961 atagacatga ccatttgtga gcgtttgtgc ctgttttagc gcactggcca tctctttcac
    58021 gcccgttaag cgcgccagac ccggttctga aaaggccgcg tgatcgctta atgccaccag
    58081 ctcgctgata tcctgcggcg taatgtcccc gtccagaacg gtcatcacgc ccgcctgccg
    58141 ggccagagtg aaggcttttt tagcaccgtc gtgccagcgt acatctgcca gcacaacatc
    58201 ccactgagag aaatcaattt cctccaacca ctctgcgtca ggcagcagat ccgggctggg
    58261 gtagttaatg attatccgct cgcctttggc atccaccatg atggcggatt gcgaagattt
    58321 cgcctggtta taccgtttgg tgtaacgggt gttaacgccc caggattcca gctctgccag
    58381 caggctgttg cccgtgtcgt catcaccgac gcgaccaata aaatcgacct gcgcccccag
    58441 ccttgccgcc gcaaccgctg ccgtcgccgc tggcccgcca ccaacttccg tataatttct
    58501 cgccacgtat ttaccgctct ccgtcggtaa tccttccacg taataaatac gatccatcac
    58561 ggtaatacct acacaagcaa cacgaatcat ggtcattcct taagcatttt gggatgcgtt
    58621 cattttaatt tcaccaattg tttaaaaagt gacggctgtc aattttttga cgataaatta
    58681 caaatacgat caaaaacaga caagcaaagc atgtcataaa tgacaaaaag tgacatcact
    58741 gtattcagga gagggttatg gcagcaatcg cgtttatcgg tttaggacaa atgggttcgc
    58801 caatggcgag caatttattg cagcaagggc atcaacttcg cgtctttgat gtgaacgccg
    58861 aagctgtgcg gcatctggta gacaaaggtg cgactcccgc cgccaacccg gcgcaggccg
    58921 ctaaagatgc cgaatttatc attaccatgt tgccgaatgg cgatctggtg cgcagcgtgt
    58981 tgttcggtga aaacggcgtt tgcgaaagct tatctaccga tgcgctggtc atcgatatgt
    59041 cgaccatcca cccgctgcaa accgataaat tgattgccga tatgcaagcc aaaggcttca
    59101 gcatgatgga tgttccggta ggccgtactt ctgcaaatgc cattaccggt actctgttac
    59161 tgctggctgg cggcaccgct gaacaagttg aacgcgccac accgatcctg atggcgatgg
    59221 gcagtgagtt gatcaacgcg ggcggtccgg gcatggggat ccgcgttaag ctcatcaaca
    59281 actacatgag catcgcgctc aatgcgcttt cggcagaagc cgctgttttg tgcgaagccc
    59341 tgaatcttcc cttcgatgtt gccgtcaagg tgatgagcgg caccgccgcc ggtaaaggcc
    59401 acttcaccac ttcctggcca aacaaagtcc tcagcgggga tctttctccc gccttcatga
    59461 tcgatcttgc ccataaggat cttggtatcg cccttgatgt cgccaaccag ctgcatgtgc
    59521 caatgccgct gggggccgcc tcacgggagg tttatagcca ggcgcgcgca gcgggtcgcg
    59581 gtcgccagga ctggtccgcc attctggaac aggtccgtgt cagtgccggg atgactgcca
    59641 aagtaaaaat gtaacgactg gataaaggaa taaatgaatg aataagtaca ccatcaacga
    59701 cattacacgc gcatcgggcg gttttgccat gctggcggtc gatcaacgcg aagccatgcg
    59761 catgatgttt gccgcggctg gggcacccgc tccagtagcc gatagcgttt taactgattt
    59821 caaagttaac gctgcaaagg ccctctcgcc ttatgcctcg gcgattctgg tggatcagca
    59881 attctgttac cgccaggtgg ttgagcaaaa cgcgattgcc aaaagttgcg ccatgattgt
    59941 cgccgccgat gagttcattc ctggcaacgg tattccggta gatagcgtgg tcattgaccg
    60001 cagaatcaat ccgctacaaa tcaaacaaga cggtggcaaa gccttaaaac tgctggtgct
    60061 gtggcgtagt gatgaagatg cgcagcaacg tctggatatg gtgaaagagt ttaacgaact
    60121 gtgccactca cacggcctgg taagcattat tgagccagtc gtccgcccac cacgtcgtgg
    60181 cgataaattc gatcgcgaac aagcgatcat cgatgccgcc aaagaactgg gcgacagtgg
    60241 cgctgacctc tacaaagttg aaatgccgct ttatggcaaa ggtccgcaac aagagcttct
    60301 cagtgcttca caacgtctga atgaccatat caatatgccg tgggtgatcc tctcatccgg
    60361 tgtcgacgaa aaactgttcc cgcgtgccgt acgcgtggca atgacagcgg gtgcatcggg
    60421 cttcctggct ggtcgtgccg tctgggcatc ggttgtcggt ttaccagaca acgagctgat
    60481 gctgcgtgac gtttgcgcac ctaaattaca acaacttggc gatatcgtcg acgaaatgat
    60541 ggctaaacgc cgttaagatg gggatacaaa aatgaaatgg tttaacacct taagtcacaa
    60601 ccgttggctg gaacaggaaa ccgaccgcat ctttgatttt ggtaaaaatt ccgtagtacc
    60661 gaccggattt ggctggttag gcaataaagg gcaaatcaaa gaagagatgg gcacccatct
    60721 gtggatcacc gctcgtatgt tgcacgttta ttccgttgct gcggcgatgg gtcgacctgg
    60781 cgcttactcg ttggttgatc acggtatcaa agccatgaac ggcgcactgc gcgataaaaa
    60841 atatggcggt tggtatgcct gcgtgaatga cgagggcgtg gtggatgcct ccaaacaggg
    60901 ctatcaacac ttctttgctc tgctgggtgc tgccagcgcc gtcactaccg gtcacccgga
    60961 agcccgtaaa cttctcgatt acgccattga aattattgag aaatacttct ggagcgaaga
    61021 agagcagatg tgcctggagt cctgggacga agccttcagc aaaaccgaag agtaccgcgg
    61081 cggcaatgcc aacatgcacg cggtggaagc cttcctgatt gtctatgacg tcacccatga
    61141 caaaaaatgg ctggatcgcg cgattcgcgt ggcttccgtg attatccacg acgtcgccag
    61201 aaataatcat tatcgcgtta acgagcactt cgatacccag tggaatccgc tgccggatta
    61261 caacaaagat aacccggcgc accgcttccg cgcattcggt ggcacgccgg gccactggat
    61321 cgaatggggc cgtttaatgc tgcacatcca cgcggccctg gaagcccgtt gtgaacagcc
    61381 gcctgcctgg ctgctagaag atgccaaagg tctatttaac gccaccgtgc gcgatgcctg
    61441 ggcacccgat ggcgcggacg ggattgttta taccgttgac tgggaaggca aaccggtggt
    61501 tcgcgaacgt gtgcgttggc ctatcgtcga agcgatgggt acggcttacg cgctctacac
    61561 cgtcaccggc gatcgccagt atgaaacctg gtatcagaca tggtgggact actgcattaa
    61621 gtacctgatg gactatgaaa atggttcctg gtggcaggag ctggatgcgg acaataaagt
    61681 caccacgaaa gtctgggacg gcaaacagga tatttatcac ctgctgcatt gcctggtgat
    61741 cccgcgtatc ccgttagccc ctggtctggc accggctgtt gccgcaggcc tgctggacat
    61801 taacgcgaaa taactgagct gaaataacgg catatcaggc tacatgccgg gtatgtcgtt
    61861 attcaataaa ggtgcctcgt atgcaaatca ccaacatgca ttgcagtgga caaaccgttt
    61921 cacttgccgc gggggattat cacgcgacta tcgtgacggt tggtgctggt ctggctgaat
    61981 taacctttca gggttgtcat ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc
    62041 atctgggtaa agtgcttatt ccgtggccga accggatcgc caacggctgt taccgctatc
    62101 aggggcagga ctatcagtta cccattaatg aacatagttc aaaagccgct attcacgggc
    62161 tgcttgcctg gcgtgactgg caaattagcg aattaaccgc caccagcgtt accctgacag
    62221 cttttctgcc gcccagttac ggctatccgt ttatgctggc ttcgcaggtt gtttattcgc
    62281 tcaacgctcg cacgggcctt tccgttgaga tcgcaagcca gaatattggt actgtgaccg
    62341 caccttatgg tgtgggtatt catccttatt taacctgcaa cttaacatct gttgatgact
    62401 atcttttcca gcttccggca gaacaggttt atgccgtcga tgagcacgca aatccgacaa
    62461 cgcttcacca cgttgatgag ctgaatttaa atttcacgcg ggcgacaaaa atagccgcga
    62521 caaaaataga ccataccttt aaaaccgcta atgatctgtg ggaaataacc attacccatc
    62581 cacaacaggc gctttcggtg agcctctgtt ccgatcaacc ctgggtgcag gtttatagcg
    62641 gtgaaaaatt acagcgtcag ggactggcag ttgaacctat gagttgcccg cctaacgcct
    62701 ttaattcagg tatcgactta ttattactgg aaccaggaaa aacgcaccgc ctgtttttta
    62761 atattcacgg gcagcacaat taattttatt aagaaaagaa cgagatcatt gtttagggtt
    62821 gtatttacac aacaggaaat attatggact tttttacgtt attttcgccg gatggggcca
    62881 tggtggaaac aagcgtggta tgtatcgtta tcgcgttaat gtttctttat acctttgtgg
    62941 ggatatgcgc gggatttggc ggtggtttaa ccactatgcc gctcgtcacg ttaatgctcc
    63001 ccgtaaaaat ggcaactccg ctctcggtga ttgtcggcac cgcgaccgcc ttgtacgcca
    63061 cctggctgtc acgtaaagaa acggattggc gatcggcaat ggtactgatt attttctcgt
    63121 ttctgggaat acccgtcggt ttatatgcgc tgtcgtattt acccgaccat tttatgaaag
    63181 tgggcttagg cggattcctt atcctttact cgttttacag catgtttatt cctcgcctgc
    63241 cgatttatga ccgtaactgg atcgccgcac ctatcggcgc agtagctggc gcattaggcg
    63301 cggcgttctc aaccaacgga ccgccgatgg ttatctacgg tatgctgcgt aacttaggcc
    63361 cggcggcatt tcgtggcact ctgaacgctt tcttcaccgc aaacaacatc gcggtgatcg
    63421 gcgggctggc gaccagcgga attttgactg tttctatctt taaactggtg ctgttttgta
    63481 ttcccaccat gatcctcggt tcactggttg gacaatatgt gcataaacgc atttcagtca
    63541 aagtgttccg tgtgatggtc tttttgttgc tgattgcctc cggttctatg ttgataaaag
    63601 gcgcactggg aattcccgcc gctgccgcac ttctgcctcc gtttaccttg ttagttatac
    63661 tgcaactggc atttggtaaa aaagtggcgg caatccgccg ggctgaagaa agtaaataat
    63721 cacttatgcg ggttttaatt tatacccgca cacattcttt aattaatgaa acctgtaaca
    63781 acaggatggc atgaaatatt agctttatac tctatggaga ttatttatgg atacgccacg
    63841 cccacaatta ccagattttc agtttcatca gaataacgac agttttaccc tacattttca
    63901 acagcgtctt attttaaccc atagcaaaga taatccttgt ttatggattg gttcaggtat
    63961 tgcggatatc gatatgttcc gcggcaattt cagcattaaa gataaactac aggagaaaat
    64021 tgcgcttacc gacgccatcg tcagccagtc accggatggt tggttaatcc atttcagccg
    64081 tggttctgac attagcgcca cgctgcgtat ctctgctgac gatcagggcc gtttattgct
    64141 ggaactacaa aacgacaacc ttaaccacaa ccgcatctgg ctgcgccttg ccgctcaacc
    64201 agaggaccat atctacggct gcggcgaaca gttttcctac ttcgatctgc gcggcaaacc
    64261 gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg
    64321 gcaggccgac tgcaaagaaa atgcgggcgg cgactattac tggactttct tcccacagcc
    64381 tacgtttgtc agcacgcaga agtattactg ccatgttgat aacagttgct acatgaactt
    64441 cgactttagc gccccggaat accatgaact ggcgctgtgg gaagacaaag ctacgctacg
    64501 ttttgaatgt gctgacacat acatctccct gctggaaaaa ttaactgccc tgctgggacg
    64561 ccagccagaa ctgcccgact ggatttatga cggggtaacg ctcggcattc agggtgggac
    64621 ggaagtgtgt cagaagaaac tggacaccat gcgtaacgcg ggtgtgaagg ttaacggtat
    64681 ctgggcgcag gactggtccg ggatccgtat gacctctttt ggcaaacgcg taatgtggaa
    64741 ctggaagtgg aacagcgaaa actatccgca actggactca cgtattaagc agtggaacaa
    64801 agagggcgtt cagttcctgg cctatatcaa cccatatgtc gccagcgata aagatctctg
    64861 cgaagaagcg gcacaacacg gctatctggc aaaagatgcc tctggcggtg actatctggt
    64921 ggagtttggc gagttttacg gcggcgttgt cgatctcact aatccggaag cctacgcctg
    64981 gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga
    65041 cttcggcgag tatctgccca ccgacactta cctgcataac ggcgtcagcg cggaaattat
    65101 gcataacgcc tggcccgcac tgtgggcgaa atgtaactac gaagcccttg aagaaacggg
    65161 caagcttggc gagatccttt tctttatgcg cgccggttct accgatagcc agaaatactc
    65221 cactatgatg tgggcgggcg accagaacgt cgactggagt ctcgacgatg gcctggcgtc
    65281 ggtcgtcccg gcagcgctgt cgctggcaat gaccggacat ggtttgcatc acagtgacat
    65341 tggcggttat accaccctgt ttgagatgaa gcgtagcaaa gagctgctgc tgcgctggtg
    65401 tgatttcagc gccttcacgc cgatgatgcg cacccacgaa ggcaaccgcc cgggcgacaa
    65461 ctggcagttt gacggcgacg cggaaactat tgcccatttt gcccgtatga ccaccgtctt
    65521 caccaccctg aaaccttacc tgaaagaggc cgtcgcgctg aatgcgaagt ccggcctgcc
    65581 ggttatgcgc ccgctgttcc tgcattacga agacgatgcg cagacctaca ccctgaaata
    65641 tcagtacctg ttaggccgcg acattctggt cgctccggtg catgaacaag gccgtagcga
    65701 ctggacgctc tatcttccgg aggataactg ggtccacgcc tggacaggtg aagcgttccg
    65761 gggcggggaa gtgaccgtta atgcgcccat cggtaaaccg ccggtctttt atcgcgccga
    65821 tagcgaatgg gcggcactgt tcgcgtcgtt aaaaagcatc taatcacgtc cgcccgtggg
    65881 gaaactcacg ggcaaaggga gaacaacgat gagtcacatc acaacggaag atccggctac
    65941 tttacgtctg ccctttaaag agaaactctc ttacggtatc ggcgatctgg cctctaacat
    66001 cctgctggat atcggcacgc tttatctttt gaagttttat accgacgttc tggggctgcc
    66061 cggcacctat ggcggcatta tctttttgat ctcgaaattc tttaccgcct ttaccgatat
    66121 gggtaccggc atcatgctcg attcccggcg caagatcggt ccaaaaggca aattccgccc
    66181 ttttattctg tatgcatcat tcccggtcac tctgctggcg atcgccaact ttgtcggcac
    66241 accgtttgat gtcaccggta aaacggtgat ggccactatt ctgtttatgc tctacgggct
    66301 gtttttcagc atgatgaact gctcctacgg cgcaatggtc cccgctatta ccaaaaaccc
    66361 caacgaacgc gcatcgctgg cagcatggcg tcagggtggc gctacattgg gcttactgct
    66421 gtgtacggtg ggattcgtgc cggttatgaa tcttatcgaa ggtaatcagc aaattggcta
    66481 tatcttcgct gccacgctgt tttcactgtt cggcctgctg tttatgtgga tctgctactc
    66541 gggcgtgaaa gagcgttatg tcgaaaccca acctaccaat ccggcgcaaa agccaggcct
    66601 gttgcaatct ttccgcgcaa ttgccggtaa ccgcccgctg ttcattctgt gcattgccaa
    66661 cctctgcact ttaggggcgt ttaacgtcaa gctcgccatc caggtctatt acacccagta
    66721 cgtacttaac gatcccatcc tgttgtcgta tatgggattt ttcagcatgg gctgtatttt
    66781 catcggcgtg ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga aggtctatat
    66841 cggcggcctg ctgatttggg tgctgggcga tctgctcaac tatttcttcg gcggcggctc
    66901 ggtcagcttt gtggcgttct cctgcctggc attcttcggc tcggcgtttg ttaacagcct
    66961 gaactgggcg ctggtttccg acaccgtcga gtacggcgag tggcgcaccg gtgtgcgttc
    67021 ggaaggtacg gtctacaccg gcttcacctt ctttcgcaaa gtatctcagg cgctggcagg
    67081 tttcttccca ggctggatgc tgacgcaaat cggctatgtg ccaaacgtcg cccaggctga
    67141 ccataccatc gaaggtttac gccagctgat cttcatctac ccaagcgcac tggcggtagt
    67201 caccattgtt gcgatgggtt gtttctacag tctgaacgag aaaatgtacg tccgcattgt
    67261 tgaagaaata gaagcccgta aacgcacgga ataaataata aacaacgccc tgcggggcgt
    67321 tataaggagt gattatgtct gaccataatc cactgacatt aaaactgaat ctgcgggaaa
    67381 aaatcgccta tggcatgggc gacgtcggtt cgaatttaat gcttagcatc ggcactctgt
    67441 atctcctcaa attttatacc gatgaattag ggatgcctgc ttactatggc gggattatct
    67501 ttctggtagc gaagtttttt accgcgttta ccgatatgct caccggattt ttactcgact
    67561 cgcggaaaaa tattgggcct aaaggcaaat ttcgtccctt tattttatat gcagcggttc
    67621 cggcggcgtt aattgcaacg ctccagttta tcgccaccac ctttagtttg ccggttaaaa
    67681 cgacgattgc cacagcactg tttatgatgt ttggactttc atacagtctg atgaactgct
    67741 cgtatggtgc gatgatcccg gcaattacca aaaaccccaa tgagcgcgcg cagctcgcag
    67801 cttaccgcca gggtggtgca accatagggc tattgatttg taccgtggcg tttattccgt
    67861 tgcagtcgct tttttctgac tccaccgtcg gttatgcctg cgcggcactg atgttctcca
    67921 ttggtggctt tatttttatg atgctgtgct acagaggcgt caaagagcat tatgtggaca
    67981 cgacgccaag cggacataaa gccagtattc tcaaatcttt ttgcgcgata tttcgtaatc
    68041 cgccattact ggttttgtgc attgctaacc tgtgtaccct ggcggcattt aatatcaaac
    68101 tggcgattca ggtctattac acccagtatg tgctgaatga tattaattta ttgtcgtgga
    68161 tgggattttt cagtatggga tgtatcctcg tcggcgtatt actggtgcca ttaactgtaa
    68221 aatgttttgg taaaaaacag gtttatttag ctggcatggt gctatgggcg gttggtgata
    68281 tcctgaatta tttctgggga agtaactctt tcactttcgt catgttctct tgtgtcgcct
    68341 tttttggcac ggcctttgta aacagcctga actgggcact ggtgccagat accgtagatt
    68401 acggtgaatg gaaaaccggt attcgtgccg aaggttctgt ttataccggt tataccttct
    68461 ttcgtaaaat ttctgccgca cttgctggct tcttgccagg cattatgctg acgcaaatcg
    68521 gttatgttcc caacatcgcg caaagtgatg cgacattaca gggtttgcgt cagctcattt
    68581 ttatctggcc ttgtgcgctg gcaattattg ctgcattaac gatgggattc ttttacacac
    68641 tcaatgaaaa acggtttgca ttgattattg aggaaatcaa ccaacgcaaa aataaagaga
    68701 tggcgacaga agaaaaaaca gcttccgtaa cgttataaat gtcaatagcc gctatttcca
    68761 tcctggtggg tggcggcctc cctacgttta aaaatggact tattatgaaa aagattaatg
    68821 caataattct attatcctct ctgacctctg cctcggtatt tgctggcgca tacgtcgaaa
    68881 acagggaggc atataatctc gcctccgatc agggcgaagt tatgctacgc gtgggttata
    68941 acttcgatat gggcgcgggt attatgttaa ccaataccta cacctttcag cgagaagatg
    69001 aactcaaaca tggctataat gaaattgaag gttggtatcc tttatttaaa ccaaccgata
    69061 aattaaccat ccagcccggt ggcttaatta atgacaagag tatcggttcg ggtggtgccg
    69121 tttatctgga tgttaactac aaatttactc cgtggtttaa cctgacggtg cgtaatcgct
    69181 ataaccataa taattacagc tctacagatt tgaacggcga attggataat aacgacacct
    69241 atgaagttgg cacctactgg aattttaaaa tcaccgagaa attctcctat acatttgaac
    69301 cacattactt catgcgagta aatgacttta atagcagcaa tggaaaagat catcactggg
    69361 aaatcactaa caccttccgt tatcgcatta atgaacactg gctcccttat ttcgaattac
    69421 gctggttaga tcgtaatgtc gaaccgtacc accgcgagca aaaccagatt cgtatcggga
    69481 cgaagtattt cttctgatgt aaaactgccg gatgcggcga aaacgcctta tccggcctac
    69541 ggttgatacg gtttccgttg gccggattaa gccacatccg gccattaatt aagcttcagc
    69601 cgccagcatc tctttcttac gttggctaat acgcttaacc agcattacgg aaaccaccgc
    69661 accacagaat gcgaagcagg ccataatgcc aaacacgatt ttgtagccaa taatccccgg
    69721 atttaaatcc agaatgtagc catacagact gaagcagaac atcgccgggg cgtaaccaat
    69781 aaagctaccc aacgccatcg ccgcgcctgt tttattttca gcaatttttg cttcgccgat
    69841 aggtgcaaaa aatacggctc gctgtgtaaa gactatcgcg ccaaagccca gcgtacatgc
    69901 catccctaaa tataccggca tactttcgtg cggcagcata atcaacagta cgagcgcagc
    69961 ggtactgatg ataaaggtgt agcatagata tttactcggc gatttcagga tcttatctga
    70021 aatcatgcca ccaatcggtc caccaatcat tttcagacag tattggttaa tgatgccgta
    70081 agcccccacc agcgcaacgg gcaatagata gatgtttttc aggaatggaa tgaagaatgt
    70141 caggccacag taaaccgcgt aaacgaagaa gacgttgaaa gcgataagcc agatagtttt
    70201 gtctttcagc accgaggtca tactggtgtt tttcgatgct ccgtcttctt ttttcacctc
    70261 aacggacggt gcctcttctt tgtcattcag aacaaagaaa atgataatgc ctacggcaat
    70321 cacaataagg gaatagaacc agatgcctgc tttaaaaccc aataagccac tgccaaacca
    70381 ggtaaatact gccaacgcag aaaatgccac cacggtatcg acaatgccac gccctgtttc
    70441 gaagaagcca aacaaccgac cttgttgttc gctattgccc aatcgactta ccgacttgag
    70501 caagaccggc cagttcatca tgtcgcaagt aacgccaaat agcgcccaga caaagaggat
    70561 gccccaatac cccggcattg tcgtcaaata aacacccagt aatcctgtcg caatgagtga
    70621 gaatgacatg gtgtatctgc gcggtagttt atcggcaaaa taaatagaca gaaaaaagcc
    70681 cactgtcgtg acaaatgagt ttaccgacat agcattacca atttgaccat tggtcaaatg
    70741 gaaatattcc tgcatcggga tataaaacgc atctttcagc gacggtaatt tataaattgt
    70801 accgccacac aatgttaata gactaaataa cgcccatttc tttttcgtga gcataacatc
    70861 ccctaagcga tttttaaaat cactgtttta aaggcaataa gataccgacc attcatatgg
    70921 aatggtttta ttaactgcgt atgagttaaa acttattagt ggttcagaag atggtttgcc
    70981 tcttgtttga tttgttgtag tacgttacgc acatgattta tctgattaat tgcatcttct
    71041 ttttctttac gtaaccgcgc atccagtaaa tgattttctg gtaggggtgt ttcactcatc
    71101 tggcgataag ggatccacgg attatcatct tcgtcttcaa agcggaacgc cggcgcatag
    71161 taatcaacct cttcttccag gccatatgcc gtcacctgcg gctcatcatc acccaggcag
    71221 ataaggtgcg ttaataacgc tttgaacggc atatcaccct gacctgaaat acaggcttta
    71281 tgacccaggc caccctcttc tttaacgatc aaggcatctt tgatatggac ctgggtaata
    71341 tgcggtgcca tcgtttttaa agcgtcgatg ggatgctcat ttgcattgat catgttcgca
    71401 aaatcaaaca gtaaggaaag agattccatc tcactttctt tgaccagcga caccagctca
    71461 tggcttttta aatcttcatg ctgttcgatc gtaaaagtca ggccgctgtc ctgatacgtt
    71521 tcccgtacat aggcaatgtc gttagcgatg atcgataata cgtcgcgcaa attaccttca
    71581 taacgtggat aaaaacgtac ggacgatgcc ccagttttca acgcaatggc gacggcttcg
    71641 tcaatagatg ccttatctga agcgctggtt tcaatatgaa tatcaaggtt cagacggcgg
    71701 gctttatccc caaaggcaga gagttcttta tcgtccatat taccaagaga aaaacgctcg
    71761 ccatccagaa catggatttt gacgccacgt agattatttt cacttgcgat atcaagtaaa
    71821 tcagcaggca acactctttc cagccgcata tttaaatgaa aggcataagc atgtaaataa
    71881 agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt tatttattgt caccataaaa
    71941 cacctacaga ttaatgatga ggtctacggc ctcaaatcgc caatattcaa tatctatctg
    72001 gacaatattg ccttgctggt cggcgcgatg cttttcaatt aagatcgctg gcatacccgc
    72061 agaaccgcca agatacttgc tgatatcgcc cggcattctg acgggtttga aaatcaattt
    72121 ttttactacc gtctcttttt ggtagcactt ttcccagact gcagaaaatg agtggttttc
    72181 aagttgttca ataaaacccg gagcaacttc aggattaata tatgtttcgt gatagaaaac
    72241 tttatgtcct tccagcgccc cccagccggt gatgcgatag agttcagttg atggtgtcac
    72301 tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta cgttttttct cggtaaaccc
    72361 ccaggaaggc tctcgtcctt gctcaattgc ggcccgctga aagctcgctg acagctccgg
    72421 actgtaatta aatcgcggct gggtcacgaa ccacccctta cgatccttac ggaagatttt
    72481 ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc atacgtttaa tgcccagcag
    72541 ttctcccagc tctctttctg aagggagttt tccacccggc gtggtgatgc catctttcaa
    72601 ccaccgatcc agtttctctt ttgcattttc tacggtggat tgattctccg ccattgttta
    72661 atccttttgg tttaaaccag atgaagcatc ttacgcttac gccaaaaaag aaagcaatta
    72721 acggtgtatg actttgcaga taattgtgac aaagctctct attgacgtaa tcacagactt
    72781 ataagggaga tactcgcatt ggtgtgcgct cacgacaggt aaaaaaaaac ctgccagcga
    72841 tggcaggttt tttatgactg aaaagcgaaa ttaatcgtct ttcggtgcgc ggttggcgcg
    72901 gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg atagaggtcg gagtcacttc
    72961 taccagttcg tcatcatcga taaactccag agcttgttcc agagtcatgc ggataggcgg
    73021 aaccagaaca acggcttcgt cagtaccgga agcacgcatg ttggtcagtt tcttaccggt
    73081 caggcagttt acagtcaggt cgttagagcg gctatgaata ccgataatct gaccttcgta
    73141 aacttctgca ccgtgaccga ggaacagctt accgcgatcc tgcagaccga acaacgcgaa
    73201 cgctaccgct ttaccctgac cgttagagat cagtacgccg ttctgacgct gacccacttc
    73261 acccggacgt acgtcgtcgt agtggctgaa ggtggagtac agcagacccg taccggaagt
    73321 catggtcatg aactcagaac ggaagccaat cagaccacgg cttgggatca cgtagtcgag
    73381 acgtacgcgg cctttaccgt ctggattcat gtttttcagg tcgcctttac gttcgcccag
    73441 cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc agcgtcacgt tttcatacgg
    73501 ctcttgttta cgaccgtcga tttcacggaa gattactttc ggacgggata ccgccagttc
    73561 gaaaccttca cgacgcatgt tttcgataag aacagacagg tgcagttcgc cacgaccaga
    73621 aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc agcgcaacgt tgtgtaccag
    73681 ttctttgttc agacgatcca ggatctgacg agacgttacg aacttacctt ctttaccgca
    73741 gaacggcgag gtgttaacgc agaagaacat agaaacggtc ggctcatcaa cggagagtgc
    73801 cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca gttcgcccag
    73861 gcccgtgatc gcaacgatat cgccagcttc cgccagatcg gtttcaatac gttccagacc
    73921 gaggtggccc agcactttac cgactttcgc gttacgggtt ttgccttcgc tatcgatgat
    73981 agtgacctgc tggttcggct tcactttacc gcgcttgatg cggccaatgc cgataacgcc
    74041 aacatagctg ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt caaggtcaac
    74101 gtccggcgca ggaacgtggt caacaatcgc ctgatacagc ggggtcatgt cttccgccat
    74161 atcttcgtgg tccagacccg cgataccgtt cagcgcagaa gcgtaaacga tcgggaagtc
    74221 cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg aatacctgat ccacaaccca
    74281 atcaggacgc gcgccagggc ggtcaacttt gttgataaca acaatcggct tcaggccgta
    74341 agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa atgcgtcaac
    74401 caccagcagc actgagtcta ccatggacat tacacgttca acttcaccac cgaagtcggc
    74461 gtgccccggg gtatcaacga tgttgatacg gtaatcattc catttgatag cggtgttttt
    74521 cgcgaggatg gtaatcccac gctctttctc caaatcgttg gagtccatca cgcgctcttg
    74581 ggtttcggca cgagagtcga acgtaccgga ttgttggagc agcttgtcta ccagggtggt
    74641 tttaccatgg tctacgtgcg cgatgatggc gatattacgc aatttttcga tcacaacttt
    74701 gcctcaggca ttagaaatag cgcgttattg tacacggatt aatcgcacta caaaacagga
    74761 tcacaaacat cctccgcaaa caagtattgc agagtccctt tgtgatcgct ttcacggagc
    74821 ataaaaaggg ttatccaaag gtcattgcac caatatggtg cttaatgttt ccattgaagc
    74881 actatattgg tgcaacattc acatcgtggt gcagcccttt tgcacgatgg tgcgcatgat
    74941 aacgcctttt aggggcaatt taaaagttgg cacagatttc gctttatctt ttttacggcg
    75001 acacggccaa aataattgca gatttcgtta ccacgacgac catgaccaat ccaggagagt
    75061 taaagtatgt ccgctgaaca cgtactgacg atgctgaacg agcacgaagt gaagtttgtt
    75121 gatttgcgct tcaccgatac caaaggtaaa gaacagcacg tcactatccc tgctcatcag
    75181 gtgaatgctg aattcttcga agaaggcaaa atgtttgacg gctcctcgat tggcggctgg
    75241 aaaggcatta acgaatccga catggtgctg atgccagacg catccaccgc agtgattgat
    75301 ccgttcttcg ccgattctac cctgattatc cgttgcgaca tccttgaacc tggtaccctg
    75361 caaggctatg accgtgaccc gcgctccatc gcgaagcgcg ctgaagatta cctgcgttcc
    75421 actggcattg ccgacaccgt actgttcggg ccagaacctg aattcttcct gttcgatgac
    75481 atccgtttcg gatcttctat ctccggttcc cacgttgcta tcgacgatat cgaaggcgca
    75541 tggaactcct ccacccaata tgaaggtggt aacaaaggtc accgtccggc agtgaaaggc
    75601 ggttacttcc cggttccgcc ggtagactct gctcaggata ttcgttctga aatgtgtctg
    75661 gtgatggaac agatgggtct ggtggttgaa gcccatcacc acgaagtagc gactgctggt
    75721 cagaacgaag tggctacccg cttcaatacc atgaccaaaa aagctgacga aattcagatc
    75781 tacaaatatg ttgtgcacaa cgtagcgcac cgcttcggta aaaccgcgac ctttatgcca
    75841 aaaccgatgt tcggtgataa cggctccggt atgcactgcc acatgtcgct gtctaaaaac
    75901 ggcgtcaacc tgttcgcagg cgacaaatac gcaggtctgt ctgagcaggc gctgtactat
    75961 attggcggcg taatcaaaca cgcgaaagcg attaatgccc tggcaaaccc gaccaccaac
    76021 tcttacaagc gtctggtccc gggctacgaa gcaccggtaa tgctggctta ctctgcacgc
    76081 aaccgttctg cgtctatccg tattccggtg gtttcttctc cgaaagcacg tcgtatcgaa
    76141 gtacgtttcc cggatccggc ggctaacccg tacctgtgct ttgctgccct gctgatggcc
    76201 ggtcttgatg gtatcaagaa caagatccat ccgggcgaag ccatggacaa aaacctgtat
    76261 gacctgccgc cggaagaagc gaaagagatc ccacaggttg ctggttctct ggaagaagca
    76321 ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg gtggcgtgtt cactgatgaa
    76381 gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg tatgactccg
    76441 catccggtag agtttgagct gtactacagc gtctaatagt tgaagttgta ctacccggcg
    76501 caacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt
    76561 tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa
    76621 atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc
    76681 agatcctcaa ctcgctgatt aacagtattt tgttaatcga tgacaacctg gcgatccatt
    76741 acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacac
    76801 cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg
    76861 aggcggggca aggttttact gataacgaag tgacgctggt catcgacggg cgttcgcata
    76921 tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag atggcaccga
    76981 tggataacca gcgccgctta agtcaggaac agctacagca cgcccagcag gttgctgccc
    77041 gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg
    77101 cggcgcagct gctcagcaaa gcattacctg acccgtcact actcgaatat accaaagtga
    77161 ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg tctgttggga ccgcaactgc
    77221 ccggtacgcg cgttaccgaa agtattcata aagtggctga acgcgtggtg acgctggtgt
    77281 cgatggaact gccggacaac gtgcggttga ttcgtgatta cgaccccagc ctgccggaac
    77341 tggcgcacga cccggatcaa attgagcaag tgctcctgaa tattgtgcgc aatgcgctac
    77401 aggcgctggg gccagagggt ggtgaaatca ttctgcgtac ccgcacagct ttccaactga
    77461 ccttacacgg cgagcgttat cggctggcgg cgcgaattga tgtggaagat aacgggccag
    77521 gtattccgcc tcatttgcag gatacactgt tttacccgat ggtcagcggc cgcgaaggtg
    77581 gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca ggcaaaattg
    77641 aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc aggaaataaa
    77701 ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg
    77761 ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acaacatttg agaacggcgc
    77821 ggaggtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat
    77881 gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc cgatgcttcc
    77941 ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg
    78001 ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gccgtggcgc tggttgagcg
    78061 cgccatcagc cattatcagg aacagcagca gccgcgtaat gttcagctta acggcccaac
    78121 gaccgatatc atcggcgaag cgccagccat gcaggacgtg ttccgtatta tcggtcggct
    78181 ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta aagaactggt
    78241 cgctcatgcc ctgcatcgcc acagtccgag agccaaagcg ccatttatcg cgctgaatat
    78301 ggcggctatc ccgaaggatt tgatcgaatc agaactgttt ggtcacgaga aaggcgcgtt
    78361 taccggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg gcacgttatt
    78421 tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc
    78481 agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat
    78541 cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc gtgaggatct
    78601 gttccaccgc ctgaacgtca tccgcgttca tctgccacca ctgcgtgagc gtcgggaaga
    78661 tattccccgc ctggcacgcc attttttaca ggttgccgcg cgcgaactgg gcgtagaagc
    78721 gaagttactg catccggaaa ccgaagccgc gctgacgcgt ctggcgtggc caggcaacgt
    78781 gcgccagctg gaaaacacct gccgctggct aacggtaatg gctgccggac aggaagtgtt
    78841 gattcaggat ttgcctggcg aactgtttga atcaacggtt gcggagagta cttcgcaaat
    78901 gcaaccggac agttgggcga cgctgttagc gcagtgggca gacagggcgc tgcgttccgg
    78961 tcatcaaaat ctgctttccg aagcgcagcc tgagctggag cggacgttac tgaccaccgc
    79021 gctgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct gggggcgtaa
    79081 cactctgacg cggaagttaa aagagctggg gatggagtga ttcacggctt gtgtgtaaag
    79141 attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat aagttcagta
    79201 tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta atctggtatc
    79261 gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc tgtgtgccgc
    79321 aatgattggg ctgtttagtt gagtttcaga ttgctgatga agtgctcttt gttcatgccg
    79381 gatgcggcgt gaacgcctta tccggtctac aaaagcaata ttttcaacag gttttgtagg
    79441 cctgataagc gtagcgcatc aggcaatctt gttctcagca cattgatggc gctttcgttt
    79501 tctactttgt aaacgaagcg ccattcacta tttaaatcac ccgagagaac tgctgcatcc
    79561 gcgctttctg gcgcagatag gtatcaaagc acatgcaaat gttgcggatc agcaagcgac
    79621 ctttcgccgt cacctgaatc cccttctcat ccacatccac cagcccatct tttgctaacg
    79681 gggcgagcag cttgagatct tccgcaaagt aatcagcgaa gtgcaaatcc cactgttgct
    79741 caatgggagc gtaatccaga cggaagttgc agatgagcga cttaatcaca tcgcggcgaa
    79801 tacagtcatc acgcgttagc gcaataccac gccacagcgc attgccttgt tcatccactt
    79861 gctgatagta gtgcttcaac tctttctggt tctgcgcata gcagtcgcca atcatactga
    79921 tggcggaaac gcccatcccc agcagatcgg tatcgccctg agtggtgtag ccctggaagt
    79981 tacggtgcag cacgccttca cgctgggcca ccgccagctc gtcatccgga cgggcaaagt
    80041 gatccatacc gataaactga tagcccgatt gcgtcaggaa ggcgatggtt tcctgcagga
    80101 tatcgagttt ttgctgcgga ctcggcaggt cagcatcttt gattttgcgc tgagcagcaa
    80161 aaatggtcgg cagatgcgcg tagttaaaga cactcagacg atcggggttc agctccgcca
    80221 cgcgtttcag ggtaaaagcg aaactctccg gcgtttgttt cggcaggccg taaatcaggt
    80281 cgatgttggt ggaggtaaag ccaatctcac gcgcatggtt aagcagtgcc aagatgaatt
    80341 cttcatcctg ctcgcggtta accagacgtt gcacttcttt gttgaagtcc tgcacgccca
    80401 tactcagacg attaaagccc tcggcgcgta aatgatcgag tacatccagt tcgatttcgc
    80461 gcggatcgac ttcgatcgaa atctccgcat cggcattgaa ctggaagttt tcgcgcagca
    80521 gcttcatcag acggctgatt tgcgctttgt tcagatacgt cggcgtacca ccgccccagt
    80581 gcagctggct caccttgcgc ccggcaaaaa gcggtgcacg atggacgatt tcttgctcca
    80641 gcgcgtccag atactgatcg gccttgtgct gctggcgagt aacaatctta ttgcaaccgc
    80701 agaagtaaca aagcttatgg cagaacggga tatgtacgta gagagataat ggacgctcag
    80761 gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg
    80821 cggtcgggta cgaggtgtat cgtggcccgg aatagttata tttctggatc agggccagat
    80881 cccagtcgat ttgctgtaca gacatgctca ctccttccga tggcgtctct ggcggctacg
    80941 gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg cgcagccact tttgtcgccg
    81001 cgacaaccgc tgtagtttaa cgaataacca caccagataa catataaccg gaagggttat
    81061 cagcagaacg ggaagaccca cggcggcaat ccgttagttg ccccgcagca gacgcatcat
    81121 gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg tcataagaga ggccgagttt
    81181 ctgcatcaac tcatcaatac gatccagttt ggcatccacc caggattgct cttcggcgct
    81241 cagggtttcg cctgcttcca gacgttccag cagcgcatcc agacgctcat ccgtttccag
    81301 taactccaac tccgcctgcg gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt
    81361 ggtgactttt tcagtcacgc ccaatggaat aggggtttta ctgccaatac gtggatcttt
    81421 cggtgcgttc tggcctttac tgcctgacgt ggtgttaccg cccgctgcgc ggctgcccgg
    81481 cgcatgacca cgacgttttt tctgacgctt acggtcacga gcttcctgat ccagctcctc
    81541 gcgtgttttt cgacgcgctt ttgcatgacc tttgctgcgt gagtttgaag atgatggttt
    81601 cataatggtt cgtcttttag ccgttttatt cagtatagat ttgcggcgga atctaacaga
//
LOCUS       DAOTRK010000006        75916 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.006, whole genome shotgun sequence.
ACCESSION   DAOTRK010000006 DAOTRK010000000
VERSION     DAOTRK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.006"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(92..763)
                     /gene="queE"
                     /locus_tag="QIE41_000432"
     CDS             complement(92..763)
                     /gene="queE"
                     /locus_tag="QIE41_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDS3529364.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            902..1043
                     /locus_tag="QIE41_000433"
                     /pseudo
     CDS             902..1043
                     /locus_tag="QIE41_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1057..1929
                     /locus_tag="QIE41_000434"
     CDS             1057..1929
                     /locus_tag="QIE41_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDS3529365.1"
                     /translation="MRYFILIFTFVCSFVAAQPTIVPSLQQQVTDLTSSLNSQEKKEL
                     TYKLESIFNNTQVQIAVLIVPTTKDETIEKYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PANPSEIESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSSAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1989..3287)
                     /gene="eno"
                     /locus_tag="QIE41_000435"
     CDS             complement(1989..3287)
                     /gene="eno"
                     /locus_tag="QIE41_000435"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDS3529366.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3375..5012)
                     /gene="pyrG"
                     /locus_tag="QIE41_000436"
     CDS             complement(3375..5012)
                     /gene="pyrG"
                     /locus_tag="QIE41_000436"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDS3529367.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5240..6031)
                     /gene="mazG"
                     /locus_tag="QIE41_000437"
     CDS             complement(5240..6031)
                     /gene="mazG"
                     /locus_tag="QIE41_000437"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDS3529368.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6103..6438)
                     /gene="mazF"
                     /locus_tag="QIE41_000438"
     CDS             complement(6103..6438)
                     /gene="mazF"
                     /locus_tag="QIE41_000438"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDS3529369.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(6438..6686)
                     /gene="mazE"
                     /locus_tag="QIE41_000439"
     CDS             complement(6438..6686)
                     /gene="mazE"
                     /locus_tag="QIE41_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDS3529370.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(6764..8998)
                     /gene="relA"
                     /locus_tag="QIE41_000440"
     CDS             complement(6764..8998)
                     /gene="relA"
                     /locus_tag="QIE41_000440"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDS3529371.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKISGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(9046..10347)
                     /gene="rlmD"
                     /locus_tag="QIE41_000441"
     CDS             complement(9046..10347)
                     /gene="rlmD"
                     /locus_tag="QIE41_000441"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDS3529372.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASEDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVAGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     CALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNDLH
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            10404..13160
                     /gene="barA"
                     /locus_tag="QIE41_000442"
     CDS             10404..13160
                     /gene="barA"
                     /locus_tag="QIE41_000442"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDS3529373.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(13401..14741)
                     /gene="gudD"
                     /locus_tag="QIE41_000443"
     CDS             complement(13401..14741)
                     /gene="gudD"
                     /locus_tag="QIE41_000443"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDS3529374.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAKRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14762..16102)
                     /locus_tag="QIE41_000444"
     CDS             complement(14762..16102)
                     /locus_tag="QIE41_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDS3529375.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(16104..17456)
                     /gene="gudP"
                     /locus_tag="QIE41_000445"
     CDS             complement(16104..17456)
                     /gene="gudP"
                     /locus_tag="QIE41_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDS3529376.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFFWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17891..18340)
                     /locus_tag="QIE41_000446"
     CDS             complement(17891..18340)
                     /locus_tag="QIE41_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDS3529377.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS"
     gene            complement(18358..19140)
                     /gene="truC"
                     /locus_tag="QIE41_000447"
     CDS             complement(18358..19140)
                     /gene="truC"
                     /locus_tag="QIE41_000447"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDS3529378.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(19140..19469)
                     /locus_tag="QIE41_000448"
     CDS             complement(19140..19469)
                     /locus_tag="QIE41_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDS3529379.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWQNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLENNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(20091..20636)
                     /gene="syd"
                     /locus_tag="QIE41_000449"
     CDS             complement(20091..20636)
                     /gene="syd"
                     /locus_tag="QIE41_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDS3529380.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRAHLASNLAEFLNQLKPLL"
     gene            20704..21552
                     /gene="queF"
                     /locus_tag="QIE41_000450"
     CDS             20704..21552
                     /gene="queF"
                     /locus_tag="QIE41_000450"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDS3529381.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21664..23028
                     /gene="ppnN"
                     /locus_tag="QIE41_000451"
                     /gene_synonym="ygdH"
     CDS             21664..23028
                     /gene="ppnN"
                     /locus_tag="QIE41_000451"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDS3529382.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            23584..24873
                     /gene="sdaC"
                     /locus_tag="QIE41_000452"
     CDS             23584..24873
                     /gene="sdaC"
                     /locus_tag="QIE41_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDS3529383.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFN"
     gene            24931..26298
                     /gene="sdaB"
                     /locus_tag="QIE41_000453"
     CDS             24931..26298
                     /gene="sdaB"
                     /locus_tag="QIE41_000453"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDS3529384.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDMIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQDTGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVATAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            26409..27164
                     /gene="xni"
                     /locus_tag="QIE41_000454"
     CDS             26409..27164
                     /gene="xni"
                     /locus_tag="QIE41_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDS3529385.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRNSGWRHQRLPDYKAGRPPMPEELHDEMPALRTAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(27219..28367)
                     /gene="fucO"
                     /locus_tag="QIE41_000455"
     CDS             complement(27219..28367)
                     /gene="fucO"
                     /locus_tag="QIE41_000455"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDS3529386.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(28395..29042)
                     /gene="fucA"
                     /locus_tag="QIE41_000456"
     CDS             complement(28395..29042)
                     /gene="fucA"
                     /locus_tag="QIE41_000456"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDS3529387.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            29589..30905
                     /gene="fucP"
                     /locus_tag="QIE41_000457"
     CDS             29589..30905
                     /gene="fucP"
                     /locus_tag="QIE41_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDS3529388.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            30938..32713
                     /gene="fucI"
                     /locus_tag="QIE41_000458"
     CDS             30938..32713
                     /gene="fucI"
                     /locus_tag="QIE41_000458"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDS3529389.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYSHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            32822..34240
                     /gene="fucK"
                     /locus_tag="QIE41_000459"
     CDS             32822..34240
                     /gene="fucK"
                     /locus_tag="QIE41_000459"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDS3529390.1"
                     /translation="MKQEVILVLDCGATNVRTIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSKLTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            34242..34664
                     /gene="fucU"
                     /locus_tag="QIE41_000460"
     CDS             34242..34664
                     /gene="fucU"
                     /locus_tag="QIE41_000460"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDS3529391.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            34722..35453
                     /gene="fucR"
                     /locus_tag="QIE41_000461"
     CDS             34722..35453
                     /gene="fucR"
                     /locus_tag="QIE41_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDS3529392.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(35497..36597)
                     /gene="rlmM"
                     /locus_tag="QIE41_000462"
     CDS             complement(35497..36597)
                     /gene="rlmM"
                     /locus_tag="QIE41_000462"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDS3529393.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(36590..36985)
                     /locus_tag="QIE41_000463"
     CDS             complement(36590..36985)
                     /locus_tag="QIE41_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDS3529394.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(37004..37921)
                     /gene="gcvA"
                     /locus_tag="QIE41_000464"
     CDS             complement(37004..37921)
                     /gene="gcvA"
                     /locus_tag="QIE41_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDS3529395.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(38272..38499)
                     /locus_tag="QIE41_000465"
     CDS             complement(38272..38499)
                     /locus_tag="QIE41_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDS3529396.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            38691..39896
                     /gene="csdA"
                     /locus_tag="QIE41_000466"
     CDS             38691..39896
                     /gene="csdA"
                     /locus_tag="QIE41_000466"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDS3529397.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            39896..40339
                     /gene="csdE"
                     /locus_tag="QIE41_000467"
     CDS             39896..40339
                     /gene="csdE"
                     /locus_tag="QIE41_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDS3529398.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTMAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAVAKQV"
     gene            complement(40390..41196)
                     /gene="tcdA"
                     /locus_tag="QIE41_000468"
     CDS             complement(40390..41196)
                     /gene="tcdA"
                     /locus_tag="QIE41_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDS3529399.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(41272..42369)
                     /gene="mltA"
                     /locus_tag="QIE41_000469"
     CDS             complement(41272..42369)
                     /gene="mltA"
                     /locus_tag="QIE41_000469"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDS3529400.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            42578..42654
                     /locus_tag="QIE41_000470"
     tRNA            42578..42654
                     /locus_tag="QIE41_000470"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42612..42614,aa:Met,seq:cat)
     gene            42688..42764
                     /locus_tag="QIE41_000471"
     tRNA            42688..42764
                     /locus_tag="QIE41_000471"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42722..42724,aa:Met,seq:cat)
     gene            42797..42873
                     /locus_tag="QIE41_000472"
     tRNA            42797..42873
                     /locus_tag="QIE41_000472"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42831..42833,aa:Met,seq:cat)
     gene            complement(42948..44201)
                     /gene="amiC"
                     /locus_tag="QIE41_000473"
     CDS             complement(42948..44201)
                     /gene="amiC"
                     /locus_tag="QIE41_000473"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDS3529401.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            44433..45764
                     /gene="argA"
                     /locus_tag="QIE41_000474"
     CDS             44433..45764
                     /gene="argA"
                     /locus_tag="QIE41_000474"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDS3529402.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(45826..47652)
                     /gene="recD"
                     /locus_tag="QIE41_000475"
     CDS             complement(45826..47652)
                     /gene="recD"
                     /locus_tag="QIE41_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDS3529403.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFSD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(47652..51194)
                     /gene="recB"
                     /locus_tag="QIE41_000476"
     CDS             complement(47652..51194)
                     /gene="recB"
                     /locus_tag="QIE41_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDS3529404.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(51187..54075)
                     /gene="ptrA"
                     /locus_tag="QIE41_000477"
     CDS             complement(51187..54075)
                     /gene="ptrA"
                     /locus_tag="QIE41_000477"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDS3529405.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRTMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(54251..57619)
                     /gene="recC"
                     /locus_tag="QIE41_000478"
     CDS             complement(54251..57619)
                     /gene="recC"
                     /locus_tag="QIE41_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDS3529406.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLEGSQELDAFVDVTPDNL
                     LHNIQSDILELENCAVAGVNIEEFSRSDNKRLLDPLDNSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(57632..57955)
                     /locus_tag="QIE41_000479"
     CDS             complement(57632..57955)
                     /locus_tag="QIE41_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDS3529407.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPAHWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(57940..58347)
                     /locus_tag="QIE41_000480"
     CDS             complement(57940..58347)
                     /locus_tag="QIE41_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDS3529408.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(58344..58907)
                     /locus_tag="QIE41_000481"
     CDS             complement(58344..58907)
                     /locus_tag="QIE41_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDS3529409.1"
                     /translation="MPVKELGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(58898..59368)
                     /locus_tag="QIE41_000482"
     CDS             complement(58898..59368)
                     /locus_tag="QIE41_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDS3529410.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(59553..60347)
                     /gene="thyA"
                     /locus_tag="QIE41_000483"
     CDS             complement(59553..60347)
                     /gene="thyA"
                     /locus_tag="QIE41_000483"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDS3529411.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(60354..61229)
                     /gene="lgt"
                     /locus_tag="QIE41_000484"
     CDS             complement(60354..61229)
                     /gene="lgt"
                     /locus_tag="QIE41_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDS3529412.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(61380..63626)
                     /gene="ptsP"
                     /locus_tag="QIE41_000485"
     CDS             complement(61380..63626)
                     /gene="ptsP"
                     /locus_tag="QIE41_000485"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDS3529413.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(63639..64169)
                     /gene="rppH"
                     /locus_tag="QIE41_000486"
     CDS             complement(63639..64169)
                     /gene="rppH"
                     /locus_tag="QIE41_000486"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDS3529414.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            64854..65543
                     /gene="mutH"
                     /locus_tag="QIE41_000487"
     CDS             64854..65543
                     /gene="mutH"
                     /locus_tag="QIE41_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDS3529415.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            65612..66325
                     /locus_tag="QIE41_000488"
     CDS             65612..66325
                     /locus_tag="QIE41_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDS3529416.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            66463..66681
                     /gene="ygdR"
                     /locus_tag="QIE41_000489"
     CDS             66463..66681
                     /gene="ygdR"
                     /locus_tag="QIE41_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDS3529417.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            66789..67829
                     /locus_tag="QIE41_000490"
     CDS             66789..67829
                     /locus_tag="QIE41_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDS3529418.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(67861..69054)
                     /gene="lplT"
                     /locus_tag="QIE41_000491"
     CDS             complement(67861..69054)
                     /gene="lplT"
                     /locus_tag="QIE41_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDS3529419.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLVAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(69047..71206)
                     /gene="aas"
                     /locus_tag="QIE41_000492"
     CDS             complement(69047..71206)
                     /gene="aas"
                     /locus_tag="QIE41_000492"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDS3529420.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAIYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            71792..72823
                     /gene="galR"
                     /locus_tag="QIE41_000493"
     CDS             71792..72823
                     /gene="galR"
                     /locus_tag="QIE41_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDS3529421.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(72830..74092)
                     /gene="lysA"
                     /locus_tag="QIE41_000494"
     CDS             complement(72830..74092)
                     /gene="lysA"
                     /locus_tag="QIE41_000494"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDS3529422.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWAYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRLVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            74214..75149
                     /gene="lysR"
                     /locus_tag="QIE41_000495"
     CDS             74214..75149
                     /gene="lysR"
                     /locus_tag="QIE41_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDS3529423.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAAKEILTPEDFHSENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(75136..75828)
                     /gene="ygeA"
                     /locus_tag="QIE41_000496"
     CDS             complement(75136..75828)
                     /gene="ygeA"
                     /locus_tag="QIE41_000496"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDS3529424.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
BASE COUNT        18496 a        20418 c        18602 g        18400 t
ORIGIN      
        1 caaaatggag gttttttaga ggcgacaaag aatataaatg agtagaagtc tttacaggtt
       61 ccacccattt ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt
      121 gcatcgacaa acgccaatta cgcgcaatgc aggtttcaat gcacaaacgt gtggcatcat
      181 ccttttggct aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgtcgcaa
      241 gcaattcatc cagtgcttcg atatcacgta cgcgtccaac tgggtgtttg atttcgttag
      301 ctcgctccag ggcttgcgac aatacttcat aaccaccacg catatttagc tttggcgaca
      361 cggtgaccca ggtattcggc gtacaacgca cttcatgagt accgctggtt tctatctggc
      421 agctaaaacc gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct
      481 caccacccgt aatcaccaca tgccgcgcgg tgtatccctg gcgaccaatg acagccagca
      541 agtcttcact gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga
      601 aaagggagac ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac
      661 agccaaccgg gcatccctgt aaacgaataa aaatggcggg gacgccggta aagtaaccct
      721 caccttgcag ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt
      781 ggataattgt taattattgc agatcctgcc acaacaatca tgtcttatta acattctgtt
      841 acaggcaggt ttaaagaaaa atgagaaaca gatcgttaat aataaaaggg agtaacgtat
      901 tatgtcagaa ataaataaag taaagggatt taatcatgtc aaaaacattc accaaaatta
      961 tacttatttt ttctgtgttc gtttttaatt ataacgaatc tcaaattacc gatgccgccg
     1021 tcaatttatt tatccagctt taatgggaca tttataatgc gctatttcat actaatattc
     1081 acattcgtgt gttcctttgt tgcagcccaa ccaactattg tcccatcatt acagcaacaa
     1141 gttacggatc ttacgagtag cttaaactca caagaaaaga aagaactgac gtacaagtta
     1201 gaatctattt tcaataatac ccaagtgcaa attgctgtat taattgttcc cacaaccaaa
     1261 gacgagacaa ttgaaaaata tgctacacgg gtttttgaca attggcgttt aggagatgcc
     1321 aaacgtaatg atgggatact gatcattgtt gcctggtcgg atcgtactgt ccgcatccag
     1381 gtaggttatg ggctggaaga aaaggtaacc gatgctctgg ctggagatat catccgtagc
     1441 aacatgatac ccgcatttaa acaacaaaaa ttagctcagg gattagagct agctataaac
     1501 gctttgaata accaactcac ttcacaacac caatatccgg ctaatccttc agaaattgaa
     1561 tcagcgtctt ccagtgatca ttattacttt gctatttttt gggtatttgc agtgatgttc
     1621 ttccctttct ggttttttca tcaaggcagt aatttttgtc gcgcatgtaa aagcagtgtc
     1681 tgtatttcag caatctatct tttagattta ttcctgttct ccgataaaac tttttccagt
     1741 gctgtatttt tcttcttttt tactttcacc accattatgg tctttacctg tttatgtgta
     1801 cttcagaaaa aagcatctgg tagaagttat cattcagaca acagcggttc cggtggcggc
     1861 tcaagtggcg gcggatttag cggcgggggc ggttcttctg gcggtggcgg cgcgtccggt
     1921 cgctggtaac tcaaccataa aaaatgccag cccgaaggct ggcattttta aatcagataa
     1981 agtcagactt atgcctggcc tttgatctct ttacgaccgt tgtacggtgc tttttcgccc
     2041 agagcttctt cgatacgaat cagctggttg tatttagcaa cgcggtcaga acggctcata
     2101 gaaccagttt tgatctggcc tgcagcagta ccaacagcca ggtcagcgat ggtagcatct
     2161 tcagtttcgc cagaacggtg agagattact gcagtgtagc cagcgtcttt cgccatcttg
     2221 attgcagcca gagtttcggt cagagaaccg atctggttga atttgatcag gatggagtta
     2281 gcgatacctt tttcgatacc ttctttcagg atcttggtgt tggttacgaa caggtcgtca
     2341 ccaaccagct ggattttgtc gcccagaact ttggtctggt atgcgaaacc gtcccagtca
     2401 gattcgtcca gaccgtcttc gatggatacg atcggatact gtttggtcag ttcttccagg
     2461 aagtgagtga attcttcaga ggtgaatgct ttgttgcctt cgccagccag aacgtattta
     2521 ccgtctttgt agaattcaga agctgcgcag tccatcgcca gagtgatgtc tttgcccagt
     2581 tcatagccag cagctttaac agcttcagcg ataacagcca gagcttcagc gttggaaccc
     2641 aggttcggcg catagccacc ttcgtcacca acagcagtgt tcatgccttt cgctttcaga
     2701 acttttgcca ggtgatggaa aacttcagaa cccatgcgga tggcttcttt cacagttttc
     2761 gcgccaaccg gctgaatcat gaattcctgg atatcaacgt tgttgtcagc gtgctcacca
     2821 ccgttgatga tgttcatcat cggaaccggc atggagtatt tgcccggagt gccgttcagt
     2881 tcagcgatgt gctcgtacag cggcatacct ttagcagctg cagcagcttt ggcgttagcc
     2941 agagatacag ccaggattgc gttcgcgccg aatttggatt tgttttcggt gccgtccagg
     3001 tcgatcatga tcttgtcaat gccagcctga tctttggcat ctttgccaat cagtgcctga
     3061 gcgatcgggc cgtttaccgc agcaacagct ttggttacgc ctttacccag gaaacgggat
     3121 ttgtcgccat cgcgcagttc cagagcttca cgggaaccag tagaagcacc tgacggagca
     3181 gctgccatac cgacgaaacc accctccaga tgtacttcgg cttcaacagt cgggttacca
     3241 cgggagtcga tgatttcacg accgatgatt tttacgattt tggacattag gttttcctca
     3301 agtcactagt taaactgaaa ctccagacaa acaacgcgta cccagggtac gcgttgccgt
     3361 tctaactttt ttacttactt cgcctgacgt ttctggaact cgctggcggc tttcacaaag
     3421 cctgcaaaca gcgggtgacc atcacgtgga gtagaagtaa actccggatg gaactggcaa
     3481 gccacgaacc acgggtgatt cggaacttcg atgatctcga ccaactgatc atccccggaa
     3541 cggcccgcaa cgcgcagacc tgcatcttca atctgtttca acagcatgtt gttgacttcg
     3601 taacggtgac gatgacgctc aacaattgtc ggcgcattgt acagctggcg aaccaggcta
     3661 tcgtcaacca actggcactg ctgtgcgccg agacgcatgg taccgccgag atcgctcttc
     3721 tcgctacgaa cttcaacgtt gccgttttca tcgcgccact cggtaatcag cgccacaacc
     3781 gggtacttac agtctggcac aaattccgta gagttggcgt tctccatgtt ggcaacatgg
     3841 cgagcgtaat caattaacgc cacctgcata cccaggcaaa tgcccagata aggaatattg
     3901 ttctcacgcg caaaacgcgc ggtcgtaatc atgccttcta cgccacgata gccgaaaccg
     3961 ccaggtacga ggattgcgtc cagaccttta agaatttcaa cgccgcgcgt ttcaacatct
     4021 tgtgaatcga tcagtttgat gttgacgctg acacgattct tcagcccacc gtgtttcagt
     4081 gcttcgatca cggatttgta agcgtccggc agttcaatgt acttgccgac cataccaatg
     4141 gtgacttcac ttaccggatt cgcttcttcg aagataacct gttcccattc ggacagattc
     4201 gcttccgggc agtttaagct gaatcgttta caaatataat cgtccagccc ctgcgatttc
     4261 aacaggcccg gaattttata gatggaatcg acgtctttca gagaaataac cgctttttcc
     4321 ggaacattac agaacaatgc aatcttcgca cgttcgttcg ccggaacggc gcgatctgaa
     4381 cgacaaatca ggatgtcagg ctggataccg atggagagca gctcttttac agagtgctga
     4441 gtcggtttgg ttttgacttc accagacgct gccatgtacg gcaccagcgt caggtgcata
     4501 aacagggtgt gctcacggcc aatttcaaca gccatctggc gaatcgcttc gaggaacggc
     4561 agggattcga tatcacctac tgtaccgccg atttcgacca gtactacgtc atgaccttcg
     4621 ccgccttcca gcacgcgctc tttgatagcg ttagtgatgt gcggaataac ctgtacggtt
     4681 gcgccgaggt agtcgccacg gcgttcttta cgcagaacgt cagagtagat acgacccgtg
     4741 gtgaagttgt tgcggcggct cattttggta cgaatgaaac gctcgtagtg ccccaggtcc
     4801 aggtcggttt cagcgccgtc ttcagtaacg aacacttccc cgtgttggat tgggctcata
     4861 gtacctggat cgacgttgat gtacggatcc agtttcatga tggtcacatt gaggccacgg
     4921 gcttcaagaa tggctgcgag ggaggctgcg gcaatgcctt tacccagaga ggatacgacc
     4981 ccgccggtca caaaaatata gttcgttgtc atgctgaacc tgagaagtta ggttgaaaag
     5041 acgatggaat aaccaggacg ggaaagcagt atacccgaac atgaccagtg ccacaaactt
     5101 tcattatccc tcctcttcgc cagcgcacta ttgaaatcag gagtgagaaa atagcccctt
     5161 tggggtaaat gtttttgacg caaatcaagc gcttgtcatt taaaaaatga cacaaatcgc
     5221 gcttgaccac gtaattccct tagagatcaa tttcctgccg ttttacctgt tgccagactt
     5281 cttccattgt ttcgaggtca acacctgtca tttccagtcc acgcgcggca acaatacgct
     5341 ccacttcgcg aaaacgacgc tcgaattttt cgttcgcttt ttgcaatgcg atttccgctt
     5401 ttgtccctaa atgacgagcc agattgaccg tggcaaacag caggtcgccc atttcctcct
     5461 ccagtttagc ctggtcgata acagcctgcc gtgcctcgta catcacctcg tcgatctctt
     5521 cgtagacttt atcgactacc ggaccaagcg tcgtccagtc aaaaccaacg ttggcgcaac
     5581 gtttctggat tttttgcgca cgcattaaag ccggtaaact acgaggaata tcgtccagcg
     5641 ccgaatgctg cgctttctgc gcgcgctctt cggttttgat ttgctcccaa cgggcaagca
     5701 cttcactact gttttcggca gaactgtcag caaaaacatg cggatggcga cgttctaatt
     5761 tatcgctgat cgcggcgcat atatcattaa agtcaaagcg cccttcttcc tgagccattt
     5821 gtgcgtaaaa caccacctgg aacagcagat cgcccagttc accgcgaaga tcgtcaaaat
     5881 cttcacgggc gatggcgtcc agcacctcgt aggtttcttc aagggtgtaa ggcgcaatgg
     5941 tggcaaatgt ctgctcttta tcccacgggc agccgttttc cggatcgcgc aggcgctgca
     6001 tgatggtgag caaacggtcg atttgattca ttagaatgtc ctgaaaattg cgggtccgtc
     6061 aggtggaaac ctgtgaccag aatagaagtg agttagtaac acctacccaa tcagcacgtt
     6121 aattttggct ttaatgagct gtaattcctc tggggcaacc gttcctttct tcgttgctcc
     6181 tcttgcccgc caggcgatac tttttacctg atcagctaac gctacgccat cacgttcctg
     6241 accggataaa acaacttcga acggatatcc ttttgattgc gttgtacaag gaacacacag
     6301 acacatacct gttttgttgt tgtacatgaa cggactcagg acaacagccg gacgatgtcc
     6361 ggcttgctcg ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg
     6421 tacgtatcgg cttaccatta ccagacttcc ttatctttcg gctctcccca gtcgatattc
     6481 tcgtggaggt tttccggcgt gatgtcgttg accagttcag caagcgtaaa tacgggctct
     6541 ttacgcactg gctcaataat taatttgcca tccaccaggt caatcttcac ttcatcatca
     6601 atattcagat tgagcgcctg cattaacgta gccgggatcc gcaccgccgg tgaatttccc
     6661 caacgcttta cgctactgtg gatcataacc ctttcctcaa accgctatca tatgtagata
     6721 cagtatatat caatctacat tgtagatacg agctaatttc ggcctaactc ccgtgcaacc
     6781 gacgcgcgtc gataacatcc ggcacctggt tgagtttacc cagcacgcga cccagcacct
     6841 gcaggttgta aatctcaatg gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac
     6901 ggctggcaac gccaagcacg ttcaccttct cgttggcgag aatggtcgtg atatcacgta
     6961 acaacccact acgatcatta gccaccacgc ggaccactag cgaatatccg gcggagtagc
     7021 tctcgcccca taccgcgtca acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt
     7081 gctcacagtc tgcacgatgc actgaaatac cgcgtccctg ggtaatgaag ccgacaatct
     7141 catctccagg aatcggctgg cagcagcgcg cgatgtggtg catcaggtta ccgacacctt
     7201 caaccaccac acgaccgtta tctttactgc ggttttgcgg cgtatagctt ttttgctgaa
     7261 gttgcttcag cgcggcggca tcctgctctt cggcactcgg cttattaaat tgcgattgca
     7321 ggaagttcac catctgattg agacggatat ccccgccacc aattgccgcc agcaactcgt
     7381 cgacatcatt gaagttgtaa cgcggcagca gatgtttttc tgcttctttc aggctgatcc
     7441 ccagatgttc cagctcgtcg tcaaggattt gccgcccagc cagaatgttt ttgtcacggt
     7501 cctgtttacg gaaccaggcg tgaattttcg aacgcccacg gctggttgtg acgtaaccga
     7561 ggtttgggtt taaccagtca cggctggggt tcggctgttt ctgggtgata atttcaatct
     7621 ggtcgcccat ctgcagctgg taggtgaacg gcacaatgcg cccgctaatt tttgccccga
     7681 tgcagcggtg cccgacatca ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg
     7741 caggcaaatc aacgacgtca cctttcggcg taaagacgta cacccggtcg tcaaagacct
     7801 ggctgcgtac ttcgtcgagc atttcgccgg aatcagccat ctcttcctgc cacgcaatca
     7861 gtttacgcag ccaggcaatc cggtcttcat gtcccgaacg tgcgccgcca gctgccgcgc
     7921 cctctttata tttccagtgc gcagcaacac ccaactctgc atcttcatgc atctgtttag
     7981 tgcggatttg gatctcaacg gttttgccac ctggtcccag aaccacggta tgaatagact
     8041 gataaccgtt tggtttcggg ttagcgacgt aatcgtcaaa ctcatccggc aggtggcgat
     8101 agtgagtgtg cactatcccc agtgcggcat agcaatcctg taaacgctcg gcgacaatac
     8161 gtaccgcacg cacatcaaac agctcatcga aggcgaggtt ctttttctgc attttgcgcc
     8221 agatgctgta gatgtgtttc ggtcgaccat acacttcagc tttaacgcct tcagctttca
     8281 tctcagcgcg cagatggccg acaaactctt cgatatagtg ttcgcggtcg agacggcgtt
     8341 catgcagcag ttttgcgatg cgtttgtatt cggtcgggtg gagataacgg aagcagtaat
     8401 cttccagctc ccatttcagt tgtccaatcc caagacggtt cgccaacggc gcgtagatat
     8461 tggtgcactc ttttgccgcc agtacacgtt catcttccgg cgcatctttt acttcgcgca
     8521 gatgagcaat acgctctgcc agtttgatga ccacgcagcg aaaatcatcg accatcgcca
     8581 ataacatccg gcgaacgtta tcgacctgtt cggaggaaac agaatcggta tgcgtcgctt
     8641 tcagctggcg gatcgccgcc atatcacgca cgccgtgaat aaggttaacg accgacttac
     8701 cgacgctctc acgcagcaca tcttcgctga ctacgttggc atcggccaga gggaacaaca
     8761 gcgccgcccg cagcgtgtca atgtccatac ttaatgtcga gaggatctcc accatctcaa
     8821 caccacgcca caataacaga ctggcatccg gatgcccctg cgtctgttgc agacaatacg
     8881 cccaggtttc ggctaagcac tcacacgact tctggctggt aatacccaga cttgcgatcc
     8941 atttttccgg atcaaattca ccagccttat tgatatgtgc acttcttacc gcaaccatcg
     9001 tcctctcctt tagggaccag acctgccgaa atcggcaaat cgtaactatt taacgcgcga
     9061 gaaaagtacc atcgattcca gatgtcccgt gtgtgggaac atatccagca tcgccagtcg
     9121 cgcaatggta tatcctgctt ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt
     9181 acaggataca taaactatac gaataggttc cagttttata atttgctgca taacacctgc
     9241 ggcacctgct cgcgcggggt ccagcaacac tttatcgaag ccgtttttcg cccacggctg
     9301 ctttgtgaca tcttcttcaa gattttcgtg ataaaacgtc acattgtgta agtcattaag
     9361 acgcgcattc tgctggcctt tttccaccag cgccggaaca ccttctacac ccaccacact
     9421 ggcagcttgt gtcgccaatg gcagtgtaaa gttgcccata ccgcagaaca gatccagtac
     9481 gcgatcttca ggttgcacat ccagccattc caacgcacac gctaccattt tttggttcac
     9541 acccgcattg acctgaataa aatcgcgcgg gctaaaagtt aagcgcaacc cgtttgagtc
     9601 ataccagggc atctcaccag cgacggtttc gagtatctca ctatcggggg cgagatacag
     9661 atccaggcct tcagaatgcg aaaagcgttc cagtttttcg cgatctgccg aacttagcgg
     9721 tgcggtatgg cgcaaaatca tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc
     9781 aagatggcgc atagcttgta agctgcccag gcatgccctg actttgggca gcaatgcttc
     9841 aagttggggc actaaaatgg ggcattgttt aacgtcgaca atgtcactgg agcccgcttt
     9901 gcgaaagccc atctgaagtt gctgtgtttt cggtaagtag ttcaaactta aacgcgcgcg
     9961 acggcgatag ccccagggaa catcggcgat cacttcagag acctcgtgtt tcattaatcg
    10021 ggcgagtgcc gcacttttgc ttcgctgctg taaatcctcg ctggcgtgtt gttgctgaca
    10081 gccgccgcat acgccaaaat gagggcagcg tggcgtttcg cgttccgggc tatcgcttaa
    10141 ccggcgtacg actttagcgc gggcgtattg ttttttatct tcagtaacag taacttccgc
    10201 gttttcctgt ggcaataatc caggaataaa taacgctttg ccgttatgtc gtgccacgcc
    10261 ctgaccaaaa gagtcgaggt cgttgactga aacagttatg atctgacgcg tcgtcgtgcg
    10321 tcgttttgca gagtagaatt gcgccattgg cgagactttc tcaatttaac agtgtgacct
    10381 taattgtccc ataacggaac tccatgacca actacagcct gcgcgcacgc atgatgattc
    10441 tgatcctggc accgaccgtc cttattggtt tattgctgag tatctttttc gttgtgcatc
    10501 gctataacga cttgcagcgt caactggaag atgccggagc cagcattatt gagccgcttg
    10561 cagtttctac tgaatatggc atgagcctgc aaaatcgcga atctatcggt cagttaataa
    10621 gcgtactgca tcgtcgccat tccgatattg ttcgcgcgat ttcggtttat gatgaaaata
    10681 accgactctt tgtcacctcc aattttcatc ttgatccctc atcaatgcag ctcggcagca
    10741 acgtgccgtt tcctcgccag ctcactgtca ctcgtgacgg cgatattatg atcctccgca
    10801 cgccgattat ttctgagagt tactcccccg acgaatcgcc cagtagcgat gccaaaaata
    10861 gtcaaaatat gttgggatat attgcgcttg agctggatct taaatcggtt cgcttgcagc
    10921 aatataaaga gatctttatt tccagcgtga tgatgctgtt ttgtatcggt attgcgctta
    10981 tttttggctg gcgcttaatg cgcgatgtaa ccggtccgat tcgcaacatg gtgaataccg
    11041 tcgaccgcat ccgtcgcggg caactcgaca gccgggtgga aggatttatg ctcggcgagc
    11101 tggatatgct gaaaaacggt atcaactcga tggcaatgtc gctggctgct tatcacgaag
    11161 agatgcagca caatatcgac caggcgacgt ccgatctgcg tgaaacgctg gagcagatgg
    11221 aaattcagaa cgttgagttg gatctggcga aaaagcgtgc ccaggaagcg gcgcgtatta
    11281 aatccgagtt tctggcaaat atgtcacacg agctgcgtac accactgaat ggtgttattg
    11341 gctttactcg cctgacgctg aaaacagaat taacaccaac gcagcgcgat cacctgaata
    11401 cgattgaacg ttcggcaaat aatttgctgg caattattaa tgatgttctc gacttctcga
    11461 aactggaagc aggtaagctg attctggaaa gtattccatt cccactacgc agcacgctgg
    11521 atgaagtcgt taccctgctg gcacattctt ctcacgataa agggctggaa ctgacgctca
    11581 atattaagag cgacgtgccc gataacgtaa ttggcgaccc actgcgatta cagcaaatca
    11641 tcactaacct ggtagggaat gcaattaaat tcaccgagaa tggcaacatt gatattctgg
    11701 tagaaaaacg tgcgctgagt aataccaaag tgcagattga agtgcagatt cgggataccg
    11761 gcattggtat tcctgaacgc gatcaatcgc gcttattcca ggccttccga caggctgatg
    11821 ccagtatttc ccgccgtcat ggtggcaccg ggctggggct ggtgattaca caaaaactgg
    11881 ttaatgaaat gggcggcgat atttcgttcc atagccagcc gaatcgcggt tcaactttct
    11941 ggttccacat taatctcgat ctgaacccga acattattat cgaagggcca tccacccagt
    12001 gcctagccgg caagcgcctg gcctatgtcg aaccaaactc cgcagcagcg caatgcacgc
    12061 tggatatttt aagtgaaacg ccgctggaag tggtttatag cccaacgttc tccgcgctgc
    12121 ctcccgcgca ttacgacatg atgttattag gcattgcggt gaccttccgc gagccgctaa
    12181 caatgcaaca tgagcgatta gcgaaagcgg tatcgatgac cgattttctg atgctggcac
    12241 ttccttgcca tgcacaagtc aatgctgaaa aacttaagca agatggtatc ggcgcgtgtc
    12301 tgctgaaacc attaacacct acgcgcctgt tacctgccct gacggaattt tgtcatcaca
    12361 aacaaaacac gcttttgcct gtaaccgatg aaagtaagct ggcaatgaca gtcatggcgg
    12421 ttgatgacaa ccccgctaac ctgaaactta tcggcgcatt gctggaagat atggtgcaac
    12481 atgtggaact ttgcgatagc gggcatcagg cggttgaacg ggcgaaacag atgccgttcg
    12541 atttgatctt aatggatatt caaatgccgg acatggacgg cattcgggcc tgcgaactca
    12601 tccaccagct cccccatcag caacaaacgc cagttatcgc ggtaacggcg catgcaatgg
    12661 ccgggcaaaa agagaagctg cttggcgcag ggatgagcga ttatctggcg aaaccgattg
    12721 aagaagagcg attgcataat ttgttgttgc gctacaagcc tggcagcggt atttcgtctc
    12781 gcgtcgtgac gcccgaagtc aacgaaattg tggtgaaccc gaatgcgacc ctcgactggc
    12841 aactggcact acgccaggca gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt
    12901 tactcgattt cctgcctgaa gttcgcaaca aagttgagga acagctggtt ggagaaaacc
    12961 cggaaggcct ggttgatttg attcataaac tgcatggcag ttgcggctat agcggtgtgc
    13021 cgcgtatgaa gaatctctgc cagctgatcg aacaacagct acgtagcggt actaaagaag
    13081 aagatttgga gccggagctg ctggaactgt tggacgagat ggataatgtc gcgcgcgaag
    13141 ccagcaaaat tctcgggtaa tggacgtctt gtcgagtcaa aataggttcc gtccaatgcc
    13201 aatgctggga gcagcacaga cagtcccctc gcccctccgg ggagagggtt agggtgaggg
    13261 gaacaggcca acgcaagtgc gaatgattac cctcaccccg cccctctccc tgaaagggcg
    13321 agggggaaaa ccggccccgt aaaccacttc gtcagtaatc tcaaaccgac ttcttatgaa
    13381 gccggttttg gttttctgtc ttaacgcacc atgcacgggc gcttgttatc aaacgtccag
    13441 ccaggaatca ggtactgcat tcccatcgca tcgtcacgcg caccaagccc atgtttctga
    13501 tacagctcgt gggctttcat cacctgatcc atatcgattt ctacgcccaa tcctggtttt
    13561 tgcggcacct gtaccagccc gcctttgatc tcaaacggtt ctttggtcag acgctgattg
    13621 ccttcctgcc agatccagtg cgtatcaata gcagtaattt tacccggtgc agcagcggct
    13681 acatgggtaa acatcgccag ggaaatatcg aagtggttgt tagagtgtga accccaggtc
    13741 agcccaaatt catggcacat ttgcgccaca cgtaccgaac cttgcatcgt ccagaaatgc
    13801 ggatccgcca gcgggatatc aacggattgt aacgagagcg tatggcccat ctgccgccag
    13861 tcggtggcga tcatattggt tgcggtcggc aggccagtcg cgcgacggaa ctccgccatc
    13921 acttcacgcc cggagaaacc ttgctccgca ccacacggat cttctgcata agccagcgac
    13981 cctttcaggt atttaccaat tttaatcgct tcgttcagcg accaggcacc gttaggatcg
    14041 agcgtaatac gcgcctgcgg gaaacgtttc gccagtgcca caatagactc ggcttcttct
    14101 tccccggcca gtacaccgcc tttcagtttg aaatcgttga agccatattt ttcatacgcc
    14161 gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt cttcgtgacg caggcgatac
    14221 cagtcacatt tatcatccgg ctggctttga tacggcagcg gtgtggcttt gcgattaccg
    14281 acgaagaaca gataaccgag catttcgact tcgctacgct gttgaccatc gcccagcagg
    14341 gatgccacgt taacccccag atgctgcccc agcagatcca gcattgccgc ttctatcccg
    14401 gtaaccacat gaatagtggt ccgcaggtca aatgtctgca aaccgcgccc gccggcatca
    14461 cgatcggcaa atgtattacg taccagcgtc agaacgtttt tgtattcacc cagcgtttta
    14521 cccaccacca gcggaatcgc atcttccagc gttttacgga ttttctcgcc gccgggaatt
    14581 tcccctacgc cagtgtgacc ggaattatct ttgataatca caatattacg cgtgaagaac
    14641 ggtgcatgtg caccactcag attcatcagc atactgtcat gacccgccac cgggatgacc
    14701 tgcatttcag taacaacagg cgtcgtaaat tgagaactca tatttatgtc ctttttcaga
    14761 atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt ccagccgggg atcaggtact
    14821 gcatcggacc tgcgtcgtta cgcgcaccgc caggcagacg tttataggcc tcatgtgcct
    14881 tttgtacctg ttcccagtcc agttccacgc ccagaccggg cgcatcagga actgcaattt
    14941 ttccgttttt aatctccagc ggattttggg tcaggcgaca atctccctcc tgccaaatcc
    15001 agtgggtatc gatagcggta ggattacccg gtgccgccgc acccacatgg gtaaacatcg
    15061 ccagagagat atcaaagtgg ttgttggaat gacagcccca ggtcagcccc cagtcgtcgc
    15121 aaagctgcgc cacgcggact gcaccggaaa gcgtccagaa gtgcggatcg gcaagtggaa
    15181 tatctactgc attgagcatt accgcatgac ccatttcacg ccagttggtg gcgatcatgt
    15241 tagtcgcgac gggcaagccg gtcgcccgcc gaaactccgc catcacttca cgaccggaga
    15301 aaccctgttc tgcgccgcat ggatcttcgg cataggtaag aacatcattc agccctttgc
    15361 acaaagaaat ggcttcatca agcagccatg caccgttggg atcaacggta atccgcgcat
    15421 ccgggaagcg tttcttcaat gcacgaacag tgtcgatttc ttgctcgcca ggtaacactc
    15481 cgcccttaag tttgaaatct ttaaagccat aacgatcctg tgaagcttcc gccagacgca
    15541 caacggcttc gctgttcatc gctttctgat ggcgcaactg ataccactca tggttgcccg
    15601 gcgtattttc cagataagga agatcggttt tggtccgatc accgatataa aacagataac
    15661 cgaggacggt aatagcctcg cgttgcttgc ctggccccaa cagttcgcag accggaacat
    15721 tcagcgcctt acctagcagg tcaagcaagg cggcttccag cgccgccacg gcattaacgc
    15781 gcaattcaaa agtccaggca cctttgccga aggtatcaaa atcggctgcc tgattacctt
    15841 tatgcacctg ctgaaccact ttattcaggc gcgcaacttc ctggcccaac accatcggaa
    15901 tagcatcgac aagcgtctga taaatcacct ctccgcctgg cgcttcacca atgccggtat
    15961 gcccggcgtt atcggtgagt accacaatat tgcgagtgaa atatgcgtta tgcgccccac
    16021 caatattaag caacatgcta tcgtgcccgg cgaccggaat gactttcata tcagtaataa
    16081 caggactgga ttgtgtcgtc atcttattgc cccgcgacag gtttcagctc gatacgcttg
    16141 atatcgccca ccagcaccag gtagctcagt accgcgatta aggcatgaac accaacataa
    16201 atcagcgccc cattaaacga gccagtcgtg ccaacgatat aaccaattgc gattggcgtg
    16261 acgatgccag aaatgttacc gaacatgttg aacaggccac cggaaagacc gctgatctct
    16321 tttggcgcgg tatctgccat tactgcccaa cccagcgccc cgatgccctt accgaagaag
    16381 gccagcgcca taaagccgat gatcatccac tcaacgttga cgtagttgca gaacaccatc
    16441 accatcgaca gcaacatgcc cattacgatc ggtgttttac gcgcaatgtt cagcgatccc
    16501 gtgcggcgca tcagccagtc ggaaataatc ccacccagca caccgccgat aaaaccgcaa
    16561 accgccggaa cggaagccac aaagcccgct ttcagaatcg acatcccgcg tgcctgcacc
    16621 agataaaccg ggaaccaggt aataaagaag taagtcaggg cgttgataca gtactgaccg
    16681 atataaacgc cgatcatcat ccgtgaccct agcagctgtt tgatctgccc ccacttcacg
    16741 ctgaacggta ctttaacttt ggtgttttgc tgatccatat tgatcagcgc gccacccgcg
    16801 gcgatgtact ccagctcttt ctgatttaca cccggatgtt gatttggctc gtggatgact
    16861 ttcagccaga aaaagctgat gacaatgccc agaccgccca taaagaagaa gacgtgtgac
    16921 cagcccactt catgagtcag ccagcccata atcggcgcga agatcaccgt tgcgaagtat
    16981 tgagcggagt taaaaataga caccgccgtt cccctttcct gcgccggaaa ccaggccgca
    17041 acaatgcgac tgttgccggg gaaagatggc gcttcagcaa gcccgaccag gaagcgcagc
    17101 gtaaacaggg caacgataat gccgaatcca ctaaagatat cgacgaagcc ttgcagcaag
    17161 gtaaacatcg accagataaa gatcgaccag aagtagacgc gttttgaacc aaaacgatcc
    17221 agcaaccaac cacctgggat ctgcccgata acataagccc atgagaaagc agagaacaca
    17281 tagcccattc ccacaggatc aaggccgata tctttggcca tttccgaacc ggcgatagag
    17341 agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa acaacatcac cactatccag
    17401 taacgagcat ttgtgcgttt ttccacactg ctcgcagcct gacttaaaga actcattgtt
    17461 gcactcctga aaattcgcgt tagccacgct cactctggac tgcgacatcg ccaggaaatc
    17521 agaggtgacg tagggtgttt tttgccgttt ttataggtcg ttcgccgaat acggcgcgtg
    17581 tttatatctg gcaatagcag tataaaaagc gcgccatagc ggctcaccgt gcaacaacac
    17641 aacattaatg cgttcaatga ggcccgattt tggcattagc cctggacggt ggaatccact
    17701 tcacggaaat gaaaacaaga acaagaaagg aagggttaaa acgaagaaat aaagaagagt
    17761 atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta tgaagcagtt
    17821 tttactggca tttgcctgaa aagattcgat tcagcaccgc taaaacggca tttaccgctc
    17881 gctgaacgta tcaggacagc agcgtgcccc aatgttcgac ccacggattt gattccgttt
    17941 ccggttccgg gttctcactg gcgtcaatca acagcatttc gccaacccgc tgagcgctct
    18001 gttcctgcaa caaggcatcg aactgtttgc caccattgca gaaattcaca taactactgt
    18061 cgccgagcgc aatcacgcca taacgcagat tcggctggaa gcccagggta tctttgattc
    18121 cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg gacgtaacca
    18181 ccagaacata cttatcctga tagggcagcc agtcgcttaa ttcaggatct tcaaataccg
    18241 ttgctttgtg gccctgcgcg gtcagaattg cttccgcttc ttccgccact aacagcgaat
    18301 ttccgtacat agtgccgaca aaaataccaa tttccgccat accgttttct ccctggatta
    18361 ggaacttatc tctccatcct gacccgatgg cgcactgaac tcaacccttt cattttcagg
    18421 aagcagaccc cgccagccaa attgtgataa cgcctgcatc caggtgtcgt ccaggcccgc
    18481 gtgaatagtc aacggctcgc cagtaaaagg atgcgtcaat gacaactgac tggcatgcag
    18541 cattaaccgc tgaaggccaa aatgttcagc accgctgcga ttctggcgta aatcaccatg
    18601 tttgctgtcg ccaataatcg ggtggcgcaa atgggcaaga tggcgacgga gctggtgctt
    18661 acgtccggtt ttcggctcca gttccaccag gccgtagcgc gtggtcgggt aacgtccggt
    18721 cgctaccggc atttctacgg tcgccagacc gcgataatgc gtcactgctg gctgtgggcc
    18781 tttatcttcg cgggcaaatt tatcagcgat tttgtccagt tcttccacca gtggataatc
    18841 cagaactgct tcttccatca accagccgcg cacaatcgca tggtaacgtt tctggatttg
    18901 gtgctgttca aactgttgtg ccagcagccg tccggcctcg ctggataatc ccatcaacaa
    18961 cacaccagaa gtgggtcggt ccagacgatg agcagtaaac acatgctggc ctatctggtc
    19021 acgcacggtt tgcatgacca ctactttctc gtcgcgatcc agccagctac ggtgaaccag
    19081 ccagccggag ggtttattta ccgcaaccag ccattcatcc tgatagagta tttccagcat
    19141 tagctcgcat catccgcaaa aagagcatcc agtttttcca gctcagccag aataagcgcg
    19201 cgttgcgggt ggtccgtcgc cagcgccatt tcataatagg gagcaacggc aaaagcgccc
    19261 ggtaacggct ggttgttttc taacaaatcg tgcattcgcg ggatcagcac ccactgcaac
    19321 cactccagtg gttccatggt gtccataaag aacggttggg tactgttaaa ttgatgcggc
    19381 tggggttcat cgttttgcca gtgctgatgt tcacgcagta atgcttcaag cgcctgcagc
    19441 tggagacgaa cgcggtcatg agtggtcata aagcaacctc aataagaaaa actgccgcga
    19501 aggatagcag gaataaaaaa agggagcact gtattcacag tgctcccggt tcgtttcgca
    19561 gcattccagc tacttttgtt gctccctgct catccttgac aacttttcct ctggccttgc
    19621 ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac caccccgatg tgatacttca
    19681 tcctgaagtg tccctggcct tcctgaccca ccgaatcatc ctgaccggtt ctcattctcc
    19741 atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc cagaagcctt
    19801 tccctgaaac accatcctgg tgtgtccttc agaagtgtca tcatcctgat gttcacttcg
    19861 ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt cgcgaagtct tacaaggcgc
    19921 ttacagacaa tgccttaagg aaaatttcat atgaaagtta aggatttaca agcaatatat
    19981 tgattttaaa tgaaaataac gattcttatc tttttaagta atcgcttatt tcctggcgat
    20041 ctcctacaga gtctgtaaga gatctctcac aaccagatgg ggtatttgga ttacagaaga
    20101 ggcttaagtt gattaaggaa ttccgcaaga tttgaggcta aatgcgcccg tttacgtgtt
    20161 cccagcgtct ctttacacac ttcaccggag agattacaca ccgagatcac ctctaactct
    20221 tcttccagcg tcgcgataaa tagcgtgggc ggcaatttta gccttttctg agtcaccaga
    20281 tgaccaatca gattttcctg cacccgacgg aagtcgtctt cactccaggt ttgcagcaat
    20341 gtcagtttaa tatcgccaaa ctgcgcgtgc atatccccgg caaactgagt cgtataaaag
    20401 gtatgaattg tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt tacattttgt
    20461 tccccggtaa acggctgggg ttgccagtat acggcgtctt cggtggttga aataatgcac
    20521 ggtgaaggaa cgccgtacag ttcctcactc aacggccagc ttttatgctc ttcatgccac
    20581 gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc gtccactaat
    20641 ttctctcttc acttaaacca gatacacttg tcgtttagtt tatctggttt atgacggtga
    20701 aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc tgactcttgg aaaatcaacc
    20761 gattaccggg atacctatga cgccagccta ctgcaaggcg ttccgcgtag tctgaatcgc
    20821 gatccgctgg gtctgaaagc ggataacctg ccttttcacg gtacggatat ctggacgcta
    20881 tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg tcgccgtcgg tcatgttgaa
    20941 cttgattaca ccagcgcaaa tctgattgag tcgaagagtt ttaagctcta tctcaacagt
    21001 tttaaccaga cgcgttttaa taactgggat gaggtgcgcc agacgctgga gcgcgactta
    21061 agcacctgcg ctcagggtaa ggttagcgtg gcgttatatc gtcttgatga actggaaggc
    21121 caaccgatag gtcatttcaa tggcacttgc attgatgacc aggatatcac tatcgataac
    21181 tatgaattca ctactgacta tctggagaat gccaccagtg gtgaaaaagt agtggaagag
    21241 acgcttgtca gccacctgct gaaatcaaac tgcctgatca cccatcaacc agattggggt
    21301 tcgatccaaa ttcagtatcg tggacgccaa attgacagag aaaaactgct gcgttacctg
    21361 gtttctttcc gccatcacaa cgagttccac gaacagtgcg tggaacgcat ctttaatgac
    21421 ctgttacgct tctgccagcc agagaaattg agcgtttacg cacgttatac ccgtcgtggc
    21481 ggtctggaca ttaacccgtg gcgcagtaat agcgattttg tcccatcgac cacaagactg
    21541 gttcggcaat aaattttttc tcaattttgc gtgctggatt cacgcagaag gttgtgaaag
    21601 gtcatcaggc agggctattg taatcaaagg gaatgacgat attcgtccca taaggagttt
    21661 ttcttgatta cacatattag cccgcttggc tccatggata tgttgtcgca gctggaagtg
    21721 gatatgctta aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa ctgttcactt
    21781 gccgtactga actccggtag cttgaccgat aacagcaaag aattgctgtc tcgttttgaa
    21841 aatttcgata ttaacgtcct gcgccgtgaa cgcggcgtaa agctggaact gattaatccc
    21901 ccggaagagg cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa cttgttcgcg
    21961 gttctgcgtg acattctctt cgtttacggg caaatccata acaccgttcg ttttcccaac
    22021 ctgaatctcg acaactccgt ccacatcact aacctggtct tttccatctt gcgtaacgct
    22081 cgtgcgctgc atgtcggtga agcgccaaat atggtggtct gctggggcgg gcactcaatt
    22141 aacgaaaacg aatatttgta tgcccgtcgc gtcgggaatc agctgggcct gcgtgagctg
    22201 aatatctgca ccggctgtgg tccgggagcg atggaagcgc caatgaaagg tgctgcggtc
    22261 ggacacgcac agcagcgtta caaagacagt cgttttattg gtatgacaga gccgtcgatt
    22321 atcgccgctg aaccgcctaa cccgctggtc aacgaattga tcatcatgcc ggatatcgaa
    22381 aaacgtctgg aagcgtttgt ccgtatcgct cacggtatca ttatcttccc tggcggtgtg
    22441 ggtacggcag aagagttgct gtatttgctg ggaattttaa tgaacccggc caacaaagat
    22501 caggttttac cattgatcct caccggcccg aaagagagcg ccgactactt ccgcgtactg
    22561 gacgagtttg tcgtgcatac gttgggtgaa aacgcgcgcc gccattaccg catcatcatt
    22621 gatgacgccg ctgaagtcgc tcgtcagatg aaaaaatcga tgccgctggt gaaagaaaat
    22681 cgccgcgaca caggcgatgc ctacagcttt aactggtcaa tgcgcattgc gccagatttg
    22741 caaatgccgt ttgagccctc tcacgagaat atggctaatc tgaagcttta cccggatcaa
    22801 cccgttgaag tgctggctgc cgacctgcgc cgtgcgttct ccggtattgt ggcgggtaac
    22861 gtaaaagaag tcggtattcg cgccattgaa gagtttggtc cttataaaat caacggcgat
    22921 aaagagatta tgcgtcgtat ggatgacctg ctacagggtt ttgttgccca gcatcgtatg
    22981 aagttgccag gctcagccta catcccttgc tacgaaatct gcacgtaatc tccctccccc
    23041 ggtgacgttt gttgccgggt tttctcgttt ttggtcacat acccatcaat ttatttcatt
    23101 tgttatatga atgtttctta ccaccctcac gcgataaata tcattacagt taatatgtca
    23161 tacaatttat gtcgtaacgc aaacgtttcc ctattttcat aaaccacatt ttatcgctac
    23221 gaaaaagaaa ttatcgccat gattaactaa aagtattgat tttttcagtt caacctacat
    23281 atattgcgcg ccccggaaga agtcagatgt cgtttaatgg gcaaatattg cccttaaatt
    23341 ctcttttact tttgatttac agagtaaagc gttgggataa tctatcttcc aagtagatta
    23401 ttgtatttgg gatcaagatc actgatagat acataacttg tgtgtatctt tccgccctca
    23461 aattattacg gcggtaaatg attaagccat cgccgataga cagatttcat ttttacggtc
    23521 aggcaccttc ccgggctgaa ctggctaaaa gctgaattat ttgcattcct ccaggagaaa
    23581 tagatggaaa cgactcaaac cagcacgatt gcgtcgaaag actctcgtag tgcctggcgc
    23641 aagacagaca ccatgtggat gctgggcctt tacggcacgg caatcggcgc gggcgtgctg
    23701 ttcctgccaa tcaacgccgg tgttggcggt atgatcccgc tgatcatcat ggctatcctt
    23761 gcgttcccga tgacattttt tgctcaccgc ggcctgactc gcttcgtact gtctggtaaa
    23821 aacccaggcg aagacatcac cgaggttgta gaagaacact ttggtattgg cgcaggtaaa
    23881 ctgattaccc tgctctactt cttcgctatc tacccgatcc tgctggttta tagcgtggca
    23941 atcaccaata cagttgaaag cttcatgtct caccagctgg gtatgacacc accgccgcgt
    24001 gcgattctgt cgctgatcct gatcgtgggt atgatgacca tcgttcgctt cggtgagcag
    24061 atgatcgtta aagcgatgag tattctggta ttcccgtttg ttggcgtact gatgctgctg
    24121 gctctgtacc tgatcccgca gtggaacggc gcagcactgg aaacgctgtc tctggacact
    24181 gcatcggcaa ccggaaacgg tctgtggatg accctgtggc tggcaattcc ggtaatggtg
    24241 ttctcgttca accactctcc gatcatctct tctttcgccg ttgcgaagcg tgaagagtac
    24301 ggcgatatgg cagaacagaa atgttcgaag atcctggcat tcgcacacat catgatggtg
    24361 ctgaccgtaa tgttcttcgt cttcagctgc gtactgagcc tgactccggc agacctggct
    24421 gcggctaaag agcagaacat ctcgattctg tcttacctgg ctaaccactt taacgcaccg
    24481 gttatcgcgt ggatggctcc gattatcgcg attatcgcta tcaccaaatc cttcctcggc
    24541 cactacctgg gcgcacgtga aggcttcaac ggtatggtga ttaaatctct gcgtggtaaa
    24601 ggtaagtcta tcgaaatcaa caagctgaac cgtatcactg cgctgttcat gctggtaacg
    24661 acctggattg ttgccaccct gaacccgagc atcctgggta tgattgaaac cctgggcggc
    24721 ccaatcatcg cgatgatcct gttcctgatg ccgatgtacg caattcagaa agtaccggca
    24781 atgcgtaagt acagcggtca catcagcaac gtattcgttg tcgtgatggg tctgattgca
    24841 atctccgcaa tcttctactc tctgttcaac taagtccttt cgcgccgctt tcgggcggcg
    24901 cttcctccgt tttaacgcga tgtatttcct atgattagcg tattcgatat tttcaaaatc
    24961 ggcattggcc cttccagttc tcataccgtt ggaccaatga aagcgggtaa acaatttacc
    25021 gacgatatga ttgcccgtaa cctgcttaaa gacgtgaccc gcgtggtggt tgacgtgtac
    25081 ggctcgctct ctctgactgg taaaggccac cacactgata tcgccattat tatgggcctg
    25141 gcgggtaacc tgccggatac cgtggatatc gattccatcc ctggttttat tcaggatgtg
    25201 aatactcatg gtcgcctgat gctggcaaat ggtcagcatg aagtggagtt cccggttgat
    25261 cagtgcatga acttccatgc cgacaacctt tctctgcatg aaaacggtat gcgcattacc
    25321 gcgctggcgg gcgataaagt cgtttacagc cagacttatt actctatcgg cggtggcttt
    25381 attgttgatg aagaacattt tggtcagcag gatagcgcac cggttgaagt tccttatccg
    25441 tacagttcag ctgccgatct gcaaaaacat tgtcaggata ccgggctgtc actctctggc
    25501 ctgatgatga aaaacgagct ggcgctgcac agcaaagaag agctggaaca gcacctggcc
    25561 aacgtctggg aagtcatgcg tggcggtatt gagcgcggta tttccaccga aggcgtgttg
    25621 cctggcaaac tgcgcgttcc acgccgtgct gcggcactac gccggatgct ggtcagccag
    25681 gataaaacca ccactgaccc gatggcggtt gttgactgga tcaacatgtt tgcactggca
    25741 gtgaacgaag agaacgctgc tggcggtcgc gtggcgactg cgccgactaa cggtgcgtgc
    25801 gggattatcc cggctgtgct ggcgtactac gataagttta tccgcgaagt gaacgctaac
    25861 tcactggctc gttacctgct ggtagccagc gccattggtt ctctttataa gatgaacgcg
    25921 tcgatttctg gtgccgaagt gggttgccag ggtgaagttg gcgtggcgtg ctctatggcg
    25981 gcagctggtc tggcagaact gttaggcgca agcccggcgc aggtgtgcat cgcggcggaa
    26041 atcgccatgg agcataacct cggtctgacg tgtgacccgg ttgctggaca ggtgcaggta
    26101 ccgtgcatcg agcgtaacgc cattgcggca gtaaaagcgg tgaacgccgc gcgtatggcg
    26161 ctgcgccgta ccagcgagcc gcgcgtctgc ctcgataaag ttatcgagac catgtacgaa
    26221 acaggtaaag atatgaacgc caagtaccgc gaaacctctc gcggtggcct ggcaatgaag
    26281 atcgttgcct gcgattaatc tctcccggag gcctcgtttt gcgaggcctc ttcccgtttt
    26341 ctcaaccagc catagcctgt tccggcatcg aatgttaccc ttatcgcctg atctttaagg
    26401 gggttatcgt ggctgttcat ttgcttattg tcgatgcact gaatcttatt cgtcgcattc
    26461 atgccgttca gggttcgccc tgtgtcgaaa cctgccagca cgcgctcgat cagctcatta
    26521 tgcacagcca accaacccac gcggtcgccg tttttgatga tgaaaaccgc aatagcggct
    26581 ggcgtcatca gcgtttacca gattacaaag cgggtcgacc gccaatgccg gaagagttgc
    26641 acgacgagat gcctgcacta cgcaccgcct ttgagcaacg cggcgtcccg tgctggtcag
    26701 ccagcggtaa cgaagccgat gacttagccg ccacgctggc ggtcaaagtg acacaggccg
    26761 ggcatcaggc aacgattgtt tcgacagata aaggctactg tcagttactt tcaccgacat
    26821 tacgtattcg tgattacttc cagaaacgtt ggctggatgc gccatttatc gataaagaat
    26881 ttggcgttca accgcagcag ttgcccgatt actgggggct tgcggggatc agcagttcaa
    26941 aggtaccggg tgttgcgggg atcggaccca aaagcgccac gcagctgctg gtcgagtttc
    27001 agagtctgga agggatatat gagaatctgg atgcggttgc cgaaaagtgg cgcaaaaaat
    27061 tagaaaccca taaagagatg gcgtttctgt gccgcgatat tgcccgctta caaaccgatt
    27121 tgcatatcga cggcaattta cagcaattgc ggttggtaag gtaacggcgt gccggatacg
    27181 gcgcaaacgt cgcatccggc attatcacat cagcgcattt accaggcggt atggtaaagc
    27241 tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac atcatccagt
    27301 gccgcctgcg ccagtgccgg aatgtcttcc ttgcgtaccc caacatcacg caaatgtggc
    27361 ggaataccga catcacggtt gagagcaaac acagcttcaa cagcggcatt acgcgcctct
    27421 tccaggctca taccttccac tttcacgccc ataacgcgcg cgatatcgcg gtacttctca
    27481 ccggtaaagt cagcgttata gcgcatgaca tgcggtaaca ggatggcgtt cgcgacaccg
    27541 tgtggagtgt tataaaacgc gcccagtgga tgcgccatac catgcaccaa ccctaaccca
    27601 acattcgaga agcccatacc cgcaacatac tgcccgagcg ccatttcttc tccggcatcc
    27661 ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga tttcaatcgc tttaatgtgc
    27721 agtgcatcgg ttagcgccca cgcgccacgg gtaatatacc cctcaatagc atgagtgagc
    27781 gcatcgacac ccgtcgcagc tttcagcgct ggaggcatac catccatcat gtcagcgtca
    27841 ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa caaacttgcg ccgtttttct
    27901 tcgtcagtga tcacgtagtt aatggtcact tctgccgcag tgcctgctgt ggtggggatt
    27961 gccagaatcg gtacactggg tttattggtc ggggaaagtc cttccaggct acgcacatcg
    28021 gcaaactccg ggttgttgct gataatgcca atcgctttac aagtatcctg tggagaacca
    28081 ccaccaatag cgatcaggta atccgcgccg ctattctgga atacaccgag cccttctttg
    28141 acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa tcgcccatgc cagccctgca
    28201 gcatccatct tatcggtcac ttttgccacc acgccgcatt gcaccagcgt tttatcggtg
    28261 acgatcagcg ccttctgata accacggcgt ttcacctcat cggttaaagc cccaacagca
    28321 ccccgaccaa accatgccgt ttcgttcaga atcattctgt tagccatcat ccttctcctt
    28381 gttgctttac gaaattactc ttcaatccgt aacccatagg ttttgaattt ctccagcact
    28441 acggcaatct cttcatcgct cagcactggc accgggtccg taatcgccag ggtcgtcagg
    28501 taaagttgcg ccagcacttc aacttcatgc gccagccata acgctttttc cagattcacc
    28561 tcacaagcga taagcccatg atgttgtaac aaagttgcct tacgattttt gagagccagc
    28621 gcaacatgtt cagaaagttc acgtgttcca aaggtcgcat aaggcgcgca aggaatagaa
    28681 ttaccgccag ccgccgcaat catgtagtga atagcgggga ttggtcggtt aagaatggaa
    28741 actgccgtgc aatgaacggc atgattgtga acaaccgcgt tggcatccgg tctgctttga
    28801 taggccgcca tatggaaacg ccattcgctt gaggggagct ttccttcctc atgtttaccg
    28861 ttgccatcaa taaagacaat atgcgactcc gtcagttttt catatggaat acctgtaggc
    28921 gtaatcagca tcccatcctg ataacgtaca ctgacgttac ccgctgtccc ctggttcagt
    28981 cccaggcggg tcatttccag gcaagtgtca ataatctgac gagcaagttt atttcgttcc
    29041 attagctacc tctctctgat tcaaaacagg gcaataatgt tgttcctttc acactattga
    29101 attagccgtt taattaccca ccatcttctt cctgattaac cagaaagaaa ttcacaagct
    29161 tatattttgt gacctggttc aactaatcat agtaaataac cgcaagttct ctttttataa
    29221 ccctattaaa aatgaccgct cttaaaaata tttatcaaaa cggtcatttt tccattcccc
    29281 ctcgccagaa atgcccgttt tcggcacaaa caattaatac ggtcatcggg tttgtgtttt
    29341 ttatgattta ttttctgaaa cgggcatgaa atttcgatta ttaaagtgat ggtagtcaca
    29401 taaagtcacc tcccagctaa taagtgtgac cgccgtcata ttacagagcg ttttttattt
    29461 gaaaatgaat ccatgagttc atttctgaca ggcaaatatt cactgatatg aagcccgaac
    29521 tcgctggttt tgcacttttg aaaacataac cgattacgtg cttaagcttc tgaacctaag
    29581 aggatgctat gggaaacaca tcaatacaaa cgcagagtta ccgtgcggta gataaagatg
    29641 cagggcaaag cagaagttac attattccat tcgcgctgct gtgctcactg ttttttcttt
    29701 gggcggtagc caataacctt aacgacattt tattacctca attccagcag gcttttacgc
    29761 tgacaaattt ccaggctggc ctgatccaat cggcctttta ctttggttat ttcattatcc
    29821 caatccctgc tgggatattg atgaaaaaac tcagttataa agcagggatt attaccggat
    29881 tatttttata tgccttgggc gctgcattat tctggcccgc tgcagaaata atgaactaca
    29941 ccttattttt agttggccta tttattattg cagccggatt aggttgtctg gaaactgccg
    30001 caaacccttt tgttacggta ttagggccgg aaagcagtgg tcacttccgc ttaaatcttg
    30061 cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt ctttggacaa agtcttattt
    30121 tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa aatgtctcca gagcaattga
    30181 gtgcgtataa acacagcctg gtattatcgg tacagacacc ttatatgatc atcgtggcta
    30241 tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt cccggcattg cagagtgata
    30301 atcacagtga cgccaaacaa ggatcgttct ccgcatcgct ttctcgtctg gcgcgtattc
    30361 gtcactggcg ctgggcggta ttagcgcaat tctgctatgt cggcgcgcaa acggcctgct
    30421 ggagctattt gattcgctac gctgtagaag aaattccagg tatgaccgca ggctttgccg
    30481 ctaactattt aaccggaacc atggtgtgct tctttattgg tcgtttcacc ggtacctggc
    30541 tcatcagtcg cttcgcacca cacaaagtcc tggcagccta cgcattaatc gctatggcac
    30601 tgtgcctgat ctcagccttc gctggcggtc atgtgggctt aatagccctg actttatgca
    30661 gtgcctttat gtcgattcag tacccaacaa tcttctcgct gggcattaaa aatctcggcc
    30721 aggacaccaa atacggttcg tccttcatcg ttatgaccat tattggcggc ggtattgtca
    30781 ctccggtcat ggggtttgtc agtgatgcag cgggcaacat ccccactgcc gaactgatcc
    30841 cggcactctg cttcgcagtc atctttatct ttgcccgttt ccgttctcaa acggcaacta
    30901 actgaacata ttttccgaat aaagtgagga atctgtaatg aaaaaaatca gcttaccgaa
    30961 aattggtatc cgcccggtta ttgacggtcg tcgcatgggt gttcgtgagt cgcttgaaga
    31021 acaaacaatg aatatggcga aagctacggc cgcactgctg accgagaaac tgcgccatgc
    31081 ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt atcgcgggta tggctgaagc
    31141 cgctgcttgc gaagaaaaat tcagcagcca gaatgtaggc ctcaccatta cggtaacgcc
    31201 ttgctggtgc tatggcagtg aaaccatcga catggatcca acccgcccga aagccatttg
    31261 gggctttaac ggcactgaac gtcccggcgc tgtttacctg gcagcggctc tggcagctca
    31321 cagccagaaa ggtatcccgg cattctccat ttacagtcat gacgttcagg atgccgatga
    31381 cacatcgatt cctgctgatg ttgaagaaaa actgctgcgc tttgcccgcg ccggtttggc
    31441 cgtcgccagc atgaaaggta aaagctatct gtcgctgggc ggcgtttcga tgggtatcgc
    31501 cggttctatt gttgatcaca acttctttga atcctggctg ggaatgaaag tccaggcggt
    31561 ggatatgacc gaactgcgtc gccgtatcga tcagaagatt tacgacgaag ccgaactgga
    31621 aatggcgctg gcctgggctg ataaaaactt ccgctatggc gaagatgaaa ataacaaaca
    31681 gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc gaaagtttac tgatggcaat
    31741 gtgtatccgc gacatgatgc aaggcaacag caaactggcc gatattggtc gcgtggaaga
    31801 atcacttggc tacaacgcca tcgctgcggg cttccaggga caacgtcact ggaccgatca
    31861 atatcccaat ggcgacaccg ccgaagcgat ccttaacagt tcatttgact ggaatggcgt
    31921 gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt aacggcgtgg caatgctaat
    31981 gggtcaccag ctcaccggca ccgctcaggt atttgctgat gtgcgtacct actggtcacc
    32041 agaagcaatt gagcgtgtaa cggggcataa actggacgga ctggcagaac acggcatcat
    32101 ccatttgatc aactccggtt ctgctgcgct ggacggttcc tgtaaacaac gcgacagcga
    32161 aggtaacccg acgatgaagc cgcactggga aatctctcag caagaggctg acgcttgcct
    32221 cgccgctacc gaatggtgcc cggcgatcca cgaatacttc cgtggcggcg gttactcttc
    32281 ccgcttcctt accgaaggcg gcgtcccgtt caccatgact cgtgtcaaca tcatcaaagg
    32341 cctgggaccg gtactgcaaa tcgcggaagg ctggagcgtg gaattgccga aggatgtgca
    32401 tgacatcctc aacaaacgca ccaactcaac ctggccaacc acctggtttg caccgcgcct
    32461 caccggtaaa gggccgttta cggatgtgta ctcggtaatg gcgaactggg gcgctaacca
    32521 cggggttcta accatcggcc acgttggcgc agactttatc actcttgcct ccatgctgcg
    32581 tatcccggta tgtatgcaca acgttgaaga gaccaaagtg tatcgtcctt ctgcctgggc
    32641 cgcgcacggc atggatattg aaggccagga ttatcgcgct tgccagaact acggcccgtt
    32701 gtacaagcgt taatacatta ctccctgatg tgatgcccgg tcgctccggc taccgggcct
    32761 gaacaagcaa gagtggttag ccggataagc aatgttatcc ggctatattg caggagcgat
    32821 tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg accaatgtca ggaccatcgc
    32881 ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg cctaatgcca gcgatatcgc
    32941 gatggaaaac aacacctggc accagtggtc tttagacgcc attttgcaac gctttgctga
    33001 ttgctgtcgg caaatcaata gtaaactgac tgaatgccac atccgcggta tcgccgtcac
    33061 cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    33121 tagctggaaa tgtccgcgaa cagcagcggt aatggacaat attgaacggt tgatctccgc
    33181 acagcagttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    33241 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctgtttat
    33301 ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca cgatggccgg
    33361 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    33421 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgtt
    33481 acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    33541 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    33601 ttccggtaca tgggaaattt taatggtccg tagcgcccag gttgatactt cgctgttaag
    33661 tcagtacgcc ggttcgacct gcgaactgga tagtcaggca gggttgtata acccaggtat
    33721 gcaatggctg gcatccggcg tgctggaatg ggtgagaaag ctgttctgga cggctgaaac
    33781 tccctggcaa atgttgattg aagaggctcg tctgatcgcg cctggcgcgg atggcgtgaa
    33841 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    33901 cacgcggggg catttctatc gcgcggcgct ggaagggtta accgcgcaat tacagcgcaa
    33961 tctacagatg ctggaaaaaa tcgggcactt taaagcctct gaattattgt tagtaggtgg
    34021 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    34081 agttctcgac gacgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    34141 aggggaattt aacagcccgg aagaagctcg cgcgcagatt cattatcagt accgttattt
    34201 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    34261 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    34321 ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg
    34381 gcctgttggt gagcgacttg ctccaggcga taatcccgtt atttgaactg gacagttatg
    34441 caccgccgct ggtgatgatg gcggcggtag aaggtgacac tctcgatcct gaagtagaac
    34501 gacgttatcg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    34561 gttttgcgtt ttatgaacgg gcacaaaaag cctttgcgat cgttatcaca ggcgaacgag
    34621 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    34681 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    34741 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    34801 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    34861 aaattctgcg caatcatggg cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    34921 atccctttca catccggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    34981 cgctcgcgtg gattgaagaa gggatggtaa tagccttaga cgccagttca acctgctggt
    35041 atctggcacg ccagttgcct gacatcaaca tccaggtctt caccaatagc catccgattt
    35101 gccatgaact cggtaaacgc gaacgcattc aactaatcag ttccggcggc acacttgagc
    35161 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg
    35221 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    35281 ccatcaacgc tgattacaaa tcaatgctat taaaacgcgc cgcgcaatcg ttgttattga
    35341 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggcatctg gatgaggtaa
    35401 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    35461 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    35521 caccgcagcc cagatacggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    35581 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    35641 attgtgtgac acttcttcgt agcgtttttt catcggcagt ttgaggttga aaatggtttc
    35701 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttcg ccggtttttc
    35761 aaccatatcg cataccatcc aggaaatatt gctgcgtgtc ggacggaatt tgaaaccatc
    35821 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    35881 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccac cagggcaagc
    35941 gcccaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    36001 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcat ctgccggaaa
    36061 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    36121 atagcagcag cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    36181 gttcgccagc acacccgcat cgcgcaaggc agcgcgtagc ggcacggtga atttacggca
    36241 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    36301 cttctctact acgccctgta acatgccgac aatgggagta atacgatctt ctggcggcaa
    36361 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    36421 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    36481 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    36541 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    36601 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcgagga
    36661 agctcacgcc gccaaccggt gtgacaaatg cccacaaacg cagatgggac agcgccaggc
    36721 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    36781 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgga
    36841 acgcctgata ttcgagaccg gtctggatcc agcccatctc aacggccccc atggttttac
    36901 ttaacacatg cgcgccaaaa gcgcccagag ccacaaaaat gaagccgcta atggcggcga
    36961 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    37021 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    37081 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcgttt ttactgacca
    37141 gtacatcatt aaacgggcaa acaagacgtc cagcctcaat ttcagattgc gccatcacgt
    37201 tatttgccag cgccactccc tgcccgtgga tagccgcttg cagcaccatg gcgctatggc
    37261 taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg gtatatgtct
    37321 gccagtcacg gcgcgacgca tcatgtaata acgtatgttt agccagatct tccggggtct
    37381 tcaagggttt ttcgccagtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    37441 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    37501 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    37561 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    37621 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    37681 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    37741 ggagtgaacg attacggcgg cggaacagtt ttagccccag aaaatcctca agagacttga
    37801 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtgaaactta
    37861 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    37921 tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    37981 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    38041 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtgt tctggtgaac
    38101 ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc
    38161 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    38221 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgatc
    38281 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    38341 tttatacgaa atcataccgg tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    38401 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    38461 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaagcgatt
    38521 aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    38581 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    38641 tatcaaagtg aattttgcgt cacgatcggt tcatcaagcc gaggagtacc atgaacgttt
    38701 ttaatcccgc gcagtttcgc gcccagtttc ccgcacttca ggatgcgggc gtctatctcg
    38761 acagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccacccga cagttttaca
    38821 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    38881 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccagat gacaaaacta
    38941 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    39001 cacgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gccaacctcg
    39061 tcccctggct gatggtcgcc caacaaacgg gagccaaagt ggtgaaattg ccgcttaatg
    39121 cgcagcgact gccggatgtc gatttgttgc cagaactgat tactccccgt agtcggattc
    39181 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    39241 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg gcggtgcatt
    39301 tccctgcgga tgttcagcaa ctggatattg atttctatgc tttctctggt cacaaactgt
    39361 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcac
    39421 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggcttc acgactcaat
    39481 ctgcgccgtg gaaactggaa gccggaacgc caaatgtcgc tggtgtcata ggattaagcg
    39541 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    39601 tagcaacgct agcggaagat gcgctggcaa aacgtcctgg cttccgctca tttcgctgcc
    39661 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    39721 tgctggcgga gtacggtatt gccctgcggg ccgggcagca ttgcgctcag ccgctactgg
    39781 cagaattagg cgtaaccggc acactgcgcg cctcttttgc gccatataat acaaagagtg
    39841 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    39901 gaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    39961 taccttcgca ccgttgacgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    40021 acagcttcct gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    40081 aaaccgcgtc tggctgggat ataccatggc tgaaaacggc aagatgcatt tctttggcga
    40141 cagcgaaggg cgcattgtgc gcggcctgct ggcggtgttg ttgactgccg ttgaggggaa
    40201 aaccgccgcc gaattgcagg cacagtcgcc gctggcattg tttgatgagc tgggattgcg
    40261 tgcgcagctt agcgcctcac gaagccaggg gttgaatgcg ttaagcgagg cgattatcgc
    40321 tgtggcgaag caggtttaag cacagaacca ggtcggataa gacgttcgcg tcgcattcga
    40381 cctgattgtt tacccctgac gggccgcttt cgccatcatc ttcttcagcg catgagaaac
    40441 cgcaacaaaa ccaaaggtgg cggtcaccat cgtcgcggca ccaaatccag atgcacaatc
    40501 catccgcttt ggcccttccg ccgtagcttt cattgcacag accgtgccat ctgactgcgg
    40561 gtacaccagc gcttcggtag agaacacgca atccacgccg agcttacctt tgctgttttt
    40621 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg ggtcctgaat
    40681 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    40741 agtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    40801 tacactatca atggcatcaa tcacgtatga ataacccacg ctcatatact gcgctacgtt
    40861 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    40921 acgctccgcc ataacttccg cttttgccag cccaacgtta tcgcgcaggg catgaatttg
    40981 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    41041 tgtgcgcgcc agcgcttccg ccgcccagga accgacaccg ccaataccga ccacacaaat
    41101 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    41161 acgctgacgc catgcatcac taattaccac agacatagca cctcagaata aaaagggcga
    41221 ggttatccct caccctgtcg tatccgtaaa aacggcatac agaatatcac atcagccgct
    41281 aaagacgtta cctgcgcccg gagccgtttt cagcacccag acacgtccat agtggttgta
    41341 ccaacctgcg cggtgcccgg cttccggccc gatcccttga tagatatcga agtgttggcc
    41401 tttgattgcg ccaccgacat ccagcgccac catcagacgc aattcgtact gaccgttaaa
    41461 tttgccgtta ttatccagca gcggcacttc tgccagcaag gtagtacctg gcggaataat
    41521 tgaacgatcg gaggcaacag aagcacggcc aaccagcggc accgcacttg cccctttcac
    41581 cggtgcaaaa gattgcggtt taaagaagac gaaagacggg ttctgttcca acagctcgcg
    41641 aacctcggct tcactgtgtg tttcgcccca gtgacgaatc gcctgcatcg acatatcttc
    41701 ttttttcact tcgccacggt cgatcagcac cttaccaatg ctgcgatatg catgaccgtt
    41761 tttccctgca tagctgaaaa agttaagcgg actgccatca ccaaagtcaa tatacccgct
    41821 accctgcaca tccataatga agttatccat cagggagttg ctgtaagcga ggatgtattt
    41881 atcgctcaat gcccccgcgt agatctccgc acgagacggc agacgaccac gttttggcgg
    41941 catacggtaa ataggatact ggaactcgcc ctggcgggta tggcgcgcct gaattaccgg
    42001 cgtgtaatag cctgtaaact gcacgttacc atagttgtcg gcaccttcca tctgccaggc
    42061 atcaatgccg aactggcgca tattgcgggt atcaccgcct gcgcgcagcc actcttgcac
    42121 cgcgttataa acattactct ggttgccata cagacgcggt gacgaattac ggatatggtt
    42181 aatttgctcg gcaaaatcac cggcgttaat cggcgcgcca acggcatctg gctggttcac
    42241 cagagagaaa ggctgggtaa atttcccgtc tttatattgc tgtccgcgat cggttggttt
    42301 ggaagagcag gcggcaagca ttgccacaac cgtgcccata agaaggtact ttacccaacg
    42361 tcctttcatt gttctcttct tcaggttaaa aaataaggcg caacgaagat aacaaaccgc
    42421 cgcgtttaat gaaatgagca gcctctccct gacgcaaatt ttgcacaaaa aataggcttt
    42481 agtgatttgt ttttgttcaa aatcatgccg aatccgtgat cggggtaaaa aaaaggttgc
    42541 atgaaaacgc gagcggagta tagtgcgcat ccacggacgc ggggtggagc agcctggtag
    42601 ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc ggcccccgca accaattaaa
    42661 atttgatgaa gtaaagcagt acggtgacgc ggggtggagc agcctggtag ctcgtcgggc
    42721 tcataacccg aaggtcgtcg gttcaaatcc ggcccccgca accaatcaaa tttgatgaaa
    42781 taaagcagta cggtgacgcg gggtggagca gcctggtagc tcgtcgggct cataacccga
    42841 aggtcgtcgg ttcaaatccg gcccccgcaa ccaattattg aacaccctaa cgggtgtttt
    42901 tttgtttctg ttctcccata aaaaaagcgc cattcagcgc ctttttatca tccccttctc
    42961 gccagcgtcg ccccatcggc aaaatacgct ttaatccccg caagaataga ctccgcaact
    43021 tcctgctgga aagtcgccgt tttcagttta cgttcttcct caacgttact gataaacgcc
    43081 gtttcgacca gaatggaggg aatatctggt gcctttagta cggcaaaccc ggcctgttca
    43141 acttgatttt tatgcagctt gttgattttc ccgagcttat tcagcaccgc tttaccaaac
    43201 ttcaggctgt cggcaatggt cagcgactgc accatatcga acatggtgtg gtcgacatag
    43261 cggtcaccgc ttttgctcac gccaccaatc aagtccgagg cgttctgggt ttgtgccaga
    43321 tattttgccg cagtactggt tgcacctttg gttgagagcg caaacacaga ggaaccgctc
    43381 ggctgacgac tggtaaaggc gtcggcatgg atagagacaa acaggtcagc acgctgtttc
    43441 tgggcttttg ctacgcgcac ttgcaacgga atgaagatgt cttcattgcg cgtcatgtac
    43501 accttcatat tgccctcttt ctcgatcaga gagcgcaaac ggcgagctat ttgcaatacc
    43561 acgtcttttt cgcgcgtttt gtatttcccc accgcaccgg agtcttcgcc accgtggcca
    43621 gggtcaagca taatgacaat cggacgatcg cgccctgctt tacctggttg tggaccactt
    43681 tgcgctggcg gcacctgctt ttcgaggtcg cctttgttgt aatcctccag cagcgccagc
    43741 agcgggtcct gcatatcctg tgcattggcc ggatagaggt ccatcaccag acgctcttta
    43801 aacccggcga ccggcgcaag ggcaaacagc tgcggtttta cgttttgctt taattcaaaa
    43861 accatgcgta cggtttgcgg gtcaaattgc ccgacgcgcg ccgacttgat gaacgggtcg
    43921 tctgcacgaa tttgtgccgc catccccttg agtaccgagt tcaggtttac atcttcgata
    43981 tccaccacca cgcgttccgg gttactcaac gcgaactgct tatatttcag ctgacgattt
    44041 gattctaccg tcacgcgggt gtagctggac gcaggccaga cgcgcaccgc cacgacctgg
    44101 ctgaccgcag ccagactgac ctgacttacg ctcaatagcc acatggcacc cgcgccttgc
    44161 agtaaacgac gacggctgat tgcagtgttg gatcctgaca tgcctctccc gagcaaaaga
    44221 aatctaagct gtgtaacaag taaacgacta atttgaccgg aaactttagc gaaagacgca
    44281 taatctgtca tctaataaac ggtaaacatt ctttttatat tcacggcatt actgataaaa
    44341 aagtcgctct cgcataaaat ttacacttgc accctgcgaa aaaacagaat aaaaatacac
    44401 taatttcgaa taatcatgca aagaggtgtg ccgtggtaaa ggaacgtaaa accgagctgg
    44461 tcgagggatt ccgccattcg gttccctata tcaataccca ccggggaaaa acgtttgtca
    44521 tcatgctcgg cggtgaagcc attgagcatg agaatttctc cagtatcgtt aatgatatcg
    44581 ggttgttgca cagcctcggc atccgtctgg tggtagtcta tggcgcacgt ccgcagatcg
    44641 acgcaaatct ggctgcgcat caccacgaac cgctgtatca caagaatata cgtgtgaccg
    44701 acgccaaaac attggaactg gtgaagcagg ctgcgggaac attgcaactg gatattactg
    44761 ctcgcctgtc gatgagtctc aataacacgc cgctgcaggg cgcgcatatc aacgtcgtta
    44821 gtggcaattt tattattgcc cagccgctgg gcgtcgatga cggcgtggat tactgccata
    44881 gcgggcgtat ccggcggatt gatgaagacg cgatccatcg tcaactggac agcggtgcaa
    44941 tagtgctaat ggggccggtc gctgtttcag tcactggcga gagctttaat ctgacctcgg
    45001 aagagattgc cactcaactg gccatcaaac tgaaagctga aaagatgatt ggtttttgct
    45061 cttcccaggg cgtcactaat gacgacggtg atattgtctc cgaacttttc cctaacgaag
    45121 cgcaagcacg ggtagaagcc caggaagaga aaggtgatta caactccggt acggtgcgct
    45181 ttttgcgtgg cgcagtgaaa gcctgccgca gcggcgtgcg tcgctgtcat ttaatcagtt
    45241 atcaggaaga tggcgcgctg ttgcaagagt tgttctcacg cgacggtatc ggtacgcaga
    45301 ttgtgatgga aagcgccgag cagattcgtc gcgcaacaat caacgatatt ggcggtattc
    45361 tggagttgat tcgcccgctg gagcagcaag gtattctggt gcgccgttct cgcgagcagc
    45421 tggagatgga aatcgacaaa ttcaccatta ttcagcgcga taacacgact attgcctgcg
    45481 ccgcgctcta tccgttcccg gaagagaaga ttggggaaat ggcctgtgtg gcagttcacc
    45541 cggattaccg cagttcatca cggggcgagg ttctgctgga acgcattgcc gctcaggcga
    45601 agcagagcgg cttaagcaaa ttatttgtgc tgaccacgcg cagtattcac tggttccagg
    45661 aacgtggatt taccccagtg gatattgatt tactgcccga gagcaaaaag cagttgtaca
    45721 actaccagcg taaatccaaa gtgttgatgg cggatttggg gtaagcgatg aaaatcgccg
    45781 gatgcgacat gcgtaacacc cgtgcgtcgc atccggcaat tacgtttatt cccgcgaact
    45841 aaacaatgcc gccagaccac tgcgccgctc agtgcgcgtg gcgattgccg cactcaaaat
    45901 acgctcatcg gcatacagcg acaaacgtcg acgcgcgcgg gttaccgcag tgtaaaccag
    45961 ctctcgtgtt actaccggcg tgcgttggct cggtaaaatc aacgccgcat ggtcgaactc
    46021 cgatccctgc gatttatgta ccgtcatcgc ccacgtagtt tcgtgctctg gcaagcgact
    46081 cggttgcaca gacttaatat tgccgtccgg catcgcaaac cagacgcgcg tcccctgccc
    46141 acgatccaga gcgataccga tatcgccatt aaacaaccca agcgcgctgt cattacgggc
    46201 aatcatcacc ggtcggcctt cgtaccaacg agagtgcgga ttacgatgaa ttttgcgctt
    46261 ctgttgcata aactgctcaa ttcgctcatt cagtccagcc acgccaaatg gcccttcccg
    46321 cagggcgcac aaaagttggt actcattgaa cgcctgaatg attaaatccg gctcggcacg
    46381 cgcttgcagc agatccagat aacgtccata acccgcaaga gcttcctcaa gcatcgcaat
    46441 ataatcttcg ccgctctgta aaagccgttt ttcgatatca gaaaaatcct gctgaaaaac
    46501 ggttttcact gccgttttat caccacggtt gatcgccgca gctaactgac caatgccaga
    46561 atcgctgccg aaacgatagc ttttttgcag caggcagaga ctgtcgcgca aagatgccgc
    46621 ttctgtgcca gttcctgccg gaacgtgagt tcccgtcagg cggcttagct gcccggcacg
    46681 ctcggcggta aagccggcat tggcataagc acaaatatcg cccagcacag ccccagcctc
    46741 aaccgaggcc agttgatcac gatcgccgag aaagatcact cgcgcatgat cgggcaaggc
    46801 gtcgatcagt ctcgacatca taggcagatc gatcattgac gcttcatcta ccaccagcac
    46861 atcaagatgc agcgggttac cggcatgatg acgtaaacgc tggctacccg gctgtgcacc
    46921 cagcaatcga tgcaaagtac tggcatcttc cggaatgcgt ttcttttgtt catcggtcag
    46981 cggtaactgt cgcaaagcct tgccgagaga ttcggttaag cgcgcggcag ctttacccgt
    47041 tggtgcagcc aaacggatac ggcagcgttc gccgtcggcc atttgaatta acgctgccag
    47101 caacttcgct acggtggtcg ttttaccggt gccagggccg ccggaaatca ccgagatccg
    47161 ccgcgtcagc gccactgccg ccgcaacttt ttgccagtta atttcatcgc ttactggaaa
    47221 aagtttgtcc agggtttgcg ccagtagagc ttcatcaacc tcaatggcat gattcacttc
    47281 gttgaaaaag cgtgccactg tgcgctcgtt acaccacatg cgattcaaat aaagacgatc
    47341 gccacagagg atcatcggcg ttggttcatc tccccggctg accgcttgtg aagccagcaa
    47401 gcattcttcc caattttgta actcaccgat ttcactgaca caggtcgcca acagcggatg
    47461 cgacgcctcg ttattttcca gtcgtgagag cggtaaacag acatgccctt ctccggcgtc
    47521 gtggcttaac agggctgccg cgagggtgac ggcaggatgt tcatctcccg ccacggtcag
    47581 ggcaaattgc acatccagcg ggcgtagctg tttgtgctcc acagcttcca gtaattgctt
    47641 ttgcaatttc attacgcctc ctccagggtc ataccggcaa acatctcatc catcaggtca
    47701 atcaacccgg cgttgggtcg ggttgtgtaa atcccttgtt gcggatgttc tttatcaacg
    47761 ccacgcagga acagatagat aacgccgcca aagtgacgat catagtcgta atcagcaatg
    47821 cgatggcgca gataacggtg cagcgccagg gtataaagct gatattgcag atcatagcgg
    47881 tgtgcctgca tcgccgctgc catagcctgt tgggtgtaag ccgaactgtc ttcacccagc
    47941 cagttggatt tatagtcgag caggtaataa cgcccttcgt ggcggaatac caggtcgata
    48001 aagcctttta acatgccgcg tacctgcatg aactccagcg gcgggcagcc agcggatagc
    48061 ggatcaaact ggcgaattag tgcatcaagc tggctggcga tgagcggttc actaatcggc
    48121 aggtaaaact ccatctccac ctgtttatcg cggtcggaaa gctgactcag gctaacaccc
    48181 gtttcattga gaggtgcctg gaggacagcc gtgatccact cggtcaacac cggttcccac
    48241 tgcgattcaa agccgccgag ttccagtttt tcctgtaccc agttcgggtc aaccggctgg
    48301 gtaaaatcga ggtcttcaaa caaactgtgc aagaatgtcc ccggtgacgc accgcgcggg
    48361 aactgatgcg gtgttaacgt cggttcttca acgacgctga ccacacctgc ggcatcaaca
    48421 tccagccgtg gcatcagatc ctgagcgata ccgtgaccac gctgctgcaa accggagtag
    48481 ctggtgacac gccagttatc gccgggcaat cgttgtagtg tcctcgcatt cagttctgca
    48541 gttatagcat cattaacctg ccagggctgg ttatcacccg tttgtgccgt ttgccaggca
    48601 atatcatcat cgcataacgc ttcaatacag gtgcgaagcc ctgccgcatc ttgcggttcc
    48661 cctttttgca gcaaacgccc gagcgcactt tggtggacgt cggtgtcacc ttttttatcg
    48721 ccacgacggc gcaccagcgg tgcaacgccg agactgcaat gccaaaccga acgcgtcagc
    48781 gccacgtaaa gcaaacgcag atcttccgcc agacgttcgg cctccgcgag gtcgacgctt
    48841 tctggcgcag cattaagatc cagaacagcc tcaaacgagt ggcgatcgtg atagaacgcc
    48901 tgatcctgga cgcggaaatt ggtaataaat ggcaaccaga ccagcggata ttccagccct
    48961 tttgatttat ggatcgtgac aatctgcacc agatgtttat cactttcgag gcgcatttgt
    49021 tggctggagg cattactgtc tggctcgagg atatgttgcg ataaccagcg taccagcgca
    49081 tgttcacttt ccagctgcgt tccggcttct tgtagcagtt cgctgatatg caagatatcg
    49141 gtaagacgcc gctcaccgcc tgccgttgcc agcaagtttt cagcaatgtt acgcgccgac
    49201 atcagcgccc gcagcatcgg cataacgcca cgtttgcgcc agatttgccg ataaccatcg
    49261 aactcttcga ctaccgcatc ccacgcatgt tcgtcattgt tcagcgtttc gatatccagc
    49321 gcgttcagcc ccatcattga cgttgccagc gcactacgca gggtgttctc acgttcgggc
    49381 gtcatcaccg cctgcaacaa ccaaagcatt tcctgcgctt ccagagtttc aaaaacactg
    49441 tcgcggttcg aaaggtaaac ggaagggatt tccagcaacg ttaaggcgtc gcgcacctgg
    49501 gcggcctcct ggcggctgcg caccagcaca ctgatgtccg aagcacgcac cggacgcgcg
    49561 tcgtcgccgt tcatcagcaa cgcttcgccc cgctgtccgg cttgtagcca gtcgcggatt
    49621 tgcgcagcac atacctgcgc catggtactt tgatagtcgc caacgccgca gctttcgcct
    49681 tccatcagcc acattttcat cgcaggctgc gtttcacctt taaacacaaa acgtaacgcc
    49741 tgatttttcc cggctgattt cactggaata aaaggaattt cgcgaaacat gaacgcgtca
    49801 tcagtctgac tgaaaagctt attcacgctg ttcaccattc ctggtgcgga gcgccagttg
    49861 gtatctaaag tgtagtgggc gtgaacttcg ctacgcgcct tcatataagt gaagatatcc
    49921 gcaccccgga atgcatatat ggcctgtttc gggtcgccaa tcagcaacaa tgcggtttcc
    49981 ggctgatggt gccagatacg gcgaaaaatt cggtactgct gggggtcggt atcctgaaat
    50041 tcatcgatca ttgccaccgg gaatcgcgta cggatcgccg ctgccaacac ctcgccgctt
    50101 tcgctacgca gcgcggaatc gagccgactt aacatgtcat caaaacccaa ttcgccacgg
    50161 cggcgttttt cacgcgctac tgtttcgcgg atctcagcca atgcgcgggt tatcagcaga
    50221 tcgcggatcg acaatggttc tgcaagcagt tgatcgatcg cctcaaacag tggatgtcgc
    50281 ggggtttccc ccccggcttt cgtgcgatct tctaagaaac gctgagagaa tttttccagc
    50341 gactccggta actgataact gttggtctct tcttctgccc aggcgctgat cttgtcgatc
    50401 catttagcct gattgctacg gttaaacttg cgtcgatcaa taccagaaga ttcgatcagc
    50461 gcatccagtt cacccaccgc gtcgcgccac tgctgtttta cggcgtcgat acgcgccaca
    50521 atttgcgcgt ggcgggaagc cagcgtttca tcatcgggcg gcggtgcttt gataaccggt
    50581 gcttcgcctt gcagataacg attaatatcg cgcagcaacg cctgcggccc tttccaggtt
    50641 tcaaagacca cctgggcaat ttcacgcggc agcgggtagc agtggcgacg ccagaaatcg
    50701 gcgcaggcct ggtagcgtag cagagactca tcttcaatca gctgctgctc aaacagcatg
    50761 ccggattcaa aggcattcag gttgagcatg cgctggcaaa aaccgtgaat agtaaagact
    50821 gccgcttcat ccatctgccg ttcggctaac aacaaccact gcgcggcttg cgctttatcg
    50881 tcgatctctt ccagcaggcg ttcgtacagt ggattgtcgg tggtttcacg cagacaggcg
    50941 atgcgcaact catggatatt gctacggata cgaccgcgca attctgccgt ggcagcctcg
    51001 gtaaaggtga ccaccagcag ttcttcaacg gtcagcgggc ggggaaaggc ggcagaaccg
    51061 cctagtccaa gtaacaggcg caaatagagc gccgcaatcg taaaggtttt gcctgtgccg
    51121 gcagaggctt caatcaggcg ctcgccctgt aagggcaagc gcaaaggatc tagtgtctcg
    51181 gcgacatcac tcattctttt cactcatcag gggcattgtt tgctgcaacg cgctgacgtt
    51241 ctcccacact ttccagcctt caggatgcac atattcggct ttcccgttct ggctgccaga
    51301 aatctgcgac agaatagcca tgccttgcgg ctcgaccacc gcctgatgga agaaatcagc
    51361 aagtttttgc ggcgtcagca gttttatctg ggccacgatt ttatcacgcg aatcgaagcg
    51421 catattgccg cgatcgaaat ctttacttaa cttcgatgct tcttcgccga gcgtttgcgg
    51481 tgcctgcagc atctgggtta ttaccgcctg ctggatttgc gcaaactctt ctggcttcat
    51541 cgtccgcaat tttgcctctg cggttgggaa aaacgccttg taacgctccc acaagaatga
    51601 aggctgttta tcattgcttt gcaacaggaa gcccatgccc cactgacgcc ccacgctcat
    51661 tggaaacgca aacacggcat agcccaactg ttcttcggta cgcaactgat tgtagaacca
    51721 cggctgtacg atctgcccca acagagagct ataggctgag ctggtgtatt catcgtagcc
    51781 agtcggtaca aataccgctg ccagtgcgga gtcggtgctg ttaccggctt tttcaaagat
    51841 gacggattgt tttttatcga ccacgacatc tttgttacga caccactccg aaccatcagc
    51901 gcccaactgt ttttgcacat cgcgtgccag cgttgttgcc tgggcttcgg tcatgttgcc
    51961 aataaccata aactctggtc gagcccctga ttttaaggcg tcgcgatagg ccagcacctc
    52021 tttcaaagta atggagggca aaattttgcg ccgttcatct cgcgagaagt acggcacttg
    52081 cgagagcatc tgggcgggca taatcgcctg ctcgaacgct ttgccctttt ctgcagaatc
    52141 catcatctgg ttataccagg acttcgcctg ctcaagctga tcttccgtag cggtatagct
    52201 aaagtacccc tcgagcaatg cctggaacag ctgcggcagg cgctgggtgt aaccattggc
    52261 attaaccata aggccgttgt tagcattggt ggaaaaactt atgccaccaa ccgacgcctg
    52321 gttgcttaac tgatcaagcg ccagccctgc gagataatca ttgagcgcaa acatcacctg
    52381 attgcgggcg ctgtccatgg ctttcgggtt acgcaaaatc aggctgacat cagctttggg
    52441 ttcgctggaa aaataacggc ttggcgcata caccacacgc agattcgact catcaacaat
    52501 cagctctgga tggtcatatt tcttctctga cttaatcagc gagaaatcgt caggaatata
    52561 ggggttaagc tctggcaaag agagcgcaat gtcggcggct tttttctgcc agtcagcgaa
    52621 agtttgtgcg ctaattttat cgacctgata cggcgcatcg acaaagtaag ccgttttgtt
    52681 gtgcggctct ttcgggctga tataccagat acgcgcattc tgcggcgtca tcatcgccag
    52741 gcgttctttt actgctttag cgtcgtaccg atcggcaata ttgactgcat ccagcgtatg
    52801 ctcaacagga acgcgaatca tggtatctgc cagccattcg acgtaatcca tatcacgggt
    52861 gattgacgga taacggaagt cgatatccag cacattcgcc agttcatcga agtattgttt
    52921 atcaatccct ttttcgcgta acagattgag gtagctaaaa atggccgcca caacctgatc
    52981 gcgattcgcc agacctttat cggttaaaga cgcagagatc gctaatacgc cgctgttgcc
    53041 gttgacgata ggatcggaat tggcgctaat gccctcaact aatccctgct tttgcagcca
    53101 gtcagaaagt gtacctgggc tgcgattacc gatcagatag gtaatcaatt catccgtttt
    53161 actacggaac ttcgctgaat tgttatcgat gcgaaactca acgcgcagaa ctttacgcgg
    53221 cagcgccggg acgtaatgaa tgataatgcc cttttgcgcg tcggtgacta ccggcacggt
    53281 gatttccggt tttttgctct ctttgttcgg cacgcgacca aaggtgtccg ccgccatttt
    53341 tgccaactcc ggcagcggtt tattgctgta aataaccgcc ttcatcagat tggcggaata
    53401 gtacttctcg tggaaatctt tcagcgcctg ctgtaccgga tttccgggtt tgtcgcttaa
    53461 agtttcgagg ttaccaccag aaaactttga accggggtgt gccgggttaa tggtttctgc
    53521 gctgacctgt gccatgcgca tcccgtcacg cgtacgcgcc atggttaatt cggcattcac
    53581 tgcattacgt tcacgttcgg cgtatttctt gtcgagcaag ggttctgcaa tagcgtccgc
    53641 caggcggtct accgcaccgg gcaaggcgtc gttctcaact tccagataga aagccgtgcg
    53701 atacggtgcc gtgctggcat tgtgactgcc gccgtgcatt ttgagatatt cggccagact
    53761 gtcagcctgc gggtactttt tcgaccccat cagactcata tgttcaaggt aatgtgccag
    53821 cccctggtac gcctcgggat cttccagcga cccaacgggc actaccagcg ccgagagcga
    53881 tttaaccgcc tgcggatcag aaaccagcaa gaccaccata ccgttatcca gacgtatagc
    53941 ctgatactgg cggttatctt tatcactttt acggatggtt tcctgaatcg gctgccatcc
    54001 cgtttctgcc tgacttaagg gtgcccaaag ggcaacaaac aacaataatg ctttgaacca
    54061 ggtgctgcgg ggcattcacg gacctcataa tcaactttat tttctggcca gattcaacag
    54121 gttaacgcaa atcatctgcc agaatttaat cttgtgctgc acgagtcagc ctatgtctat
    54181 atatccatca gtccgtgact ggtgcgcatc ataaagcaag cagatatatt gcgcaatttt
    54241 tatacagcac tcatgactga ttaaagcgaa acagcggtaa caggaaacgt tgcgactgtt
    54301 caacgatggc ctccattgtc tctggtgtta attgccgcca gagccgttga taccagatat
    54361 catcaccttc gccacgcacc atcatgttgc cttcgtaagc ctgaaggaat ttcgtacggg
    54421 ctttttgtaa cgtggaatcg tcatccagca tggcatcgtt ttgcgcgtca taacaggttt
    54481 ttagccacgc gccgccactt tcaggtaaca ccagcaatgg cgcggacatt ccttcacgat
    54541 acccctcaat cagttgtgag aggtaatgca aagcctgttc ggctgcaagc ggcggaaaac
    54601 gccactcgcc gtctttgcgt agaaaaaggc gactttcacc attaccaccg ctggcacagt
    54661 agacaaggtg ttccagccaa agttgcattc cctgcgccac acttaataaa gagggacgcc
    54721 agcgcaacag gccatccggc tgcacctgcg gcaaccagcc agttatctgc acaccgttgc
    54781 aggcgagatc aatttccata ctctgccccg gctggcgaca ggcaataact ctgtcggcaa
    54841 gctgctgcat ctcctggcac tgtgtttccc agaaaatttc accaaaagcg ccatacggta
    54901 aatcccctgc cgcccggaag cggcggaaca agcgttcggc atcatcctgc tcaaccagtg
    54961 cattcaataa ctgctgattg atttgataac ggctaagccc ttccagaata aatggctcgg
    55021 tgtcggggat ttcgctgtct tcagtacgga agttcacctg caaacgcatc tggaagaacg
    55081 cccgtaccgg atgtgcccag aatcgttgta gcgtttccag cggcacggtt tccggtaagg
    55141 taaacggcag cggctgaaca aattcagaat gtgctttacc agcctggctg gccgcaggta
    55201 gccattcacg agcatagctt tgtcgttcgc ctggctggta gttttgcgga tcaaacggca
    55261 tccgggtatg gaggcaagta agatgcgctt ttacccttgc ctcgctttca tcacagttga
    55321 gcgcttcatc gcccggtaga taatggcttt gcccgatgta gtcgatcagt tcctgcacca
    55381 gtaccgacgg gaaacgttca ctgttatcct gaatagaacg cccgatatag ctgatataga
    55441 gtttttgctg cgcggaaatt aacgcttcca ggaacagata gcggtcgtca tcgcgacgac
    55501 tacggtcgcc acgcttcggt ttctggctca tcaggtcaaa gcccaatggc gcaagctggc
    55561 gtggataaac gccgtcgttc attcccagca ggcaaaccac tttgaatggg atggaacgca
    55621 ttggcatcag agtacaaatg ttaaccggcc cggcgagaaa acgctggctg atacgttcct
    55681 gatccaggcg ctgtgccagt tcatcacgca atagtgacag cggcaccgcg tcgccatact
    55741 gcgcacctaa accttcggcg ataatcgcct gccattgttg ttcgatcagc gtcatcgccg
    55801 cttcggtttc cgcatccggc aggaagaagg cgttgagcat atcgcgacaa accggcaacc
    55861 actcttccag cggacgctcc tgcgccagcc cgcgacgcca gatgtttagc tgcattagca
    55921 gtgaagccag atgccccacc agttctgcaa ttaagccgct cgattcatca taaggtagaa
    55981 ccgattgcca ctcgccctgc gcactctcca tcgcgtagcc cagcaacatg cgcgtcaggc
    56041 caaaccgcca ggtgtgttga ccggtagcgg ggagttccag ctcgcgaacg ttgtcgtcat
    56101 ctatccccca acgaatgccg gattcgttga cccactggcg taaataacgc agcccttctt
    56161 cggtgatgtc aaaccgcgcc gccagcaccg gcacatccag taatgccaga acgtcctccg
    56221 acacaaagcg gctgtctggc aacgataaca ggctgataaa cgcttcaagt acaggatgcg
    56281 actgccgcgc ccgacggtcg gaaatggcgt aaggtaggta acgatccgca ggtgcgctac
    56341 caaacactgc ctgaataaac ggactgtagc tgtcgatgtc ggccaccatc acgatgatgt
    56401 cgcgcggagt caatgtcggg tcttcctcca gcattgccag caggcgatcg tgtaaaactt
    56461 caacttcacg ctgcgggcta tggcaaacgt ggaaggtgat actgttatcc agtggatcaa
    56521 gcaggcgttt gttatcgcta cgggaaaact cttcgatatt cacacccgca acagcgcagt
    56581 tttcaagttc cagaatgtca gactgaatgt tatgcagcag gttatctggc gtgacatcga
    56641 caaaggcgtc cagctcctgg ctgccctcca gatcagaaag gagataaatg tagtcgcgcc
    56701 caagcttgcc ccatgaggcc agcagcgggt tgccgacatc ctgttcacca tcgctgttaa
    56761 agagctgccc ggcattttcg ctgtcgcgaa atagcggtaa ttcgcgatct tcaaaactgt
    56821 gtcgacgctg acgagtcagc agtttcgcca gataagcagg atctttaatg tcgccccagt
    56881 aataacggca ggggttggta aacaggagat ggatttcaat atgtttaccc agcgcctgta
    56941 gcgcctggag ataaacaggc ggtaacgcgg aaataccgca tataaagacg cgcgaaggta
    57001 gccccggggg gcaggtcgtc gcggactcca gcgtttcgat aaagcgctga tagagattgg
    57061 cgcggtgcca gcgcggttgc ccgagttgat gggtatattc caccaacgcc ttccacaacg
    57121 gcgcttgcca agcctgtgct tctcccagcc cttcaaccaa atgtcctgtt tcccactgtg
    57181 ccagccagtc cggacgatag accagatact ggtcaaacag gtccgccgct tttgaagaaa
    57241 gctggaacag ttttcgcttg tcgctatcgt cagtcagata atgccgcaac agggtaaagt
    57301 cttcgcgctc caacagttgc ggcagcagag tcatcagttt ccagctcatg ctctgtttgt
    57361 taaaggcact ctctttgggg atctccggta acacccggac gaacatatcc catataaagc
    57421 tcgctggcag cggaaaatca atatttgccg caataccaaa cttttgcgac agggtcattt
    57481 gcagccactg cgccataccg gtactttgca ccagaatcat ctctggttcg aaaggatcgt
    57541 ccagccgttc gcgttcgaca ataaactcca tcaacgcttc cagcacgtcc agacgattgg
    57601 aatggtagac ccttaacata gcggctcctg actactgacg attcgggcaa tgcaggcggg
    57661 tcatctcgcc ttctctgccc ccgggtgaaa ctagcgtaac gctgatgctg acacatcccg
    57721 cttgcgatgt ctgcattcgg ttgacctgcc agtgggcagg tggcgaaatc gcacgcagtt
    57781 gcgtttgctg ccagccatgc cgccagagct gttggtactg gtttctactg gcaagactgt
    57841 tcattaatgt tcgctggaaa cccgataacg cagtgacaat catcaccatc agcaccattg
    57901 ccaacatcac ctccgggagg ctaaagcctt gttgattctt caaggaagct gacataacgc
    57961 cctctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa tgtttccatc
    58021 gatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaatca ataaggcctc
    58081 attatctgcc aataaacgca gacaaacccg ggcaccggtg gcagcgtaca gtaagcactg
    58141 aactgcgggc tgcgtctgcc aggagtgcat ttttccccac tccagcgccg actgaacaat
    58201 ggcctggcgg cgcaatgact gactttccat gctcacgcga gaggcaaaac tgcgatcctg
    58261 ctgactcatt ccttgtaata gcaagctacc caaaaccagc agcatcagga ccagagccag
    58321 tgacgaaaca cccttttcgc ggttcacagg ttgaatcctg tcacgctata gctggcatcc
    58381 accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa caccggcgag
    58441 aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc atccggatta
    58501 gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt ttccagcaca
    58561 tgctccttca gacgaaatcc aatctggtcg gactctttta ccggttcgcg atcccagata
    58621 ccgttactgt tcgcatccca ctgcacaatg acacagtcac cctgtccgac aatttccagc
    58681 ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt cgcaacggta
    58741 aatacccgca gccagatttc atcttccagc gccagcttac gggtgctcgt taaactttca
    58801 cgctgtaacg caggcagaaa gcgtgctgcc cccagcaaca atacgctact gatcgccata
    58861 gcaatcaaca cttccagcag agaaaaacct agctctttta caggcatcct tctgtttctc
    58921 cttgctgaca aagccggagt cttccccacg gcgaaaccac cagccaccac tcgcccgttg
    58981 agtttttgaa gcgaatatgc ccggcccatg cggtattgcg taggccaaag aaagcaagcg
    59041 aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac
    59101 tgccatggca ggtattggcc ccagcagcgg aactcacaag gcaccataac gtcccctccc
    59161 tgataacact gatactgtgg tcgcggttat gccagttggc atcttcacgt aaatagagca
    59221 aatagtcccg cgcctggctg gcggtttgcc atagccgttg cgactgctgc cagtattgcc
    59281 agccatagag tccacttgcg cttagcatga ccagaatcag catcgcgacc agcgtttcaa
    59341 tcagcgtata accacgttgt gttttcatgc cagcagtatg gagcgaggag aaaaaaagac
    59401 gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt tgcaggaggt
    59461 tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa accgacgcac
    59521 actggcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaatgccc
    59581 ggatgtggat cgtatccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg
    59641 ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga
    59701 gtttgatcca tatgattgct gtacaggtgc gtgtcgccac cggtccagac aaaatcaccc
    59761 acttccagat cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg
    59821 aacggcaggc cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg
    59881 tctgccacat agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg
    59941 cctacgttcc acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc
    60001 agtaccgtag tgatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt
    60061 ttaccataca ctggcccgag gtcgccgttt tcatcggccc attcgtccca gatggtgaca
    60121 ttgttttcgt gtagataagc aatgttagtg tcgccctgca gaaaccacag cagttcatgg
    60181 atgatggaac gcaggtggca acgtttagtt gtcaccagcg ggaatccatc ctgcaggtta
    60241 aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt
    60301 gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa
    60361 acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc
    60421 atcgggatgg aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc
    60481 gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac
    60541 aagcctgaga ctgctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac
    60601 agcaccacac cttccagcag cagctcgtaa agctgtgatg ggtggcgcgg cagcacaccg
    60661 taagtgtcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta
    60721 cgggagccag ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca
    60781 ccgttaataa aattgcccag acgcccggcg ccaagaccaa acggaatgag tggtgcgata
    60841 aaatcagaga cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg
    60901 ataacgccaa tcaggccacc gtggaaagac atgccgccgt cccagacacg gaacagatac
    60961 agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat acgtccaccg
    61021 aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagccg
    61081 ctgcccggac gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga
    61141 tacatcaggc cgtaccagtg aagcgctacg ggtcctattg agaaaatgac cggatcaaac
    61201 tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga
    61261 aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat
    61321 agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc
    61381 tataaccctc cgcgaatcaa cccgcccatg ccgcgacgct ccataaaggc tgcaacctga
    61441 tggcgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg
    61501 gcataatcaa tgcgccgcag taggtatttt acgcgcgcta cagaacgtcc gttcatagac
    61561 agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca
    61621 ccgcacaaac ggagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct
    61681 cgtaacattg caggatgaag actgtcataa atgttcgcca cccgggtatt gttgcgatca
    61741 acggccagga tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt
    61801 gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt
    61861 ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg
    61921 cgtgcttcgt caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc
    61981 gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg
    62041 agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa
    62101 ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac
    62161 atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacggaaa accactttgc
    62221 agcatgaatg ggatttcagt gcgataaagt cctatgccat caatacggct gcccagtttt
    62281 tcttcatgtt ccgggcttaa accggcattg agcatgactt ttatacgctc gccacttttt
    62341 aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta
    62401 attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga
    62461 taaccatcga caatcagcgt ccgacgatgc agcaccgaag gctgaatatc cgcgcccatc
    62521 acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc tgcgccatcg
    62581 cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagcgttgt cgctgacagt
    62641 tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca
    62701 agatgaaaca gcaatcgctg ccccagcgca cgtaaatcgc cagcccgctc tttgagatag
    62761 ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac
    62821 tctgccaccg agcctttatc aacctcggca aacaattcgc gccgcagccg ggtgtccgaa
    62881 agcaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgcacc ggcggcaaag
    62941 cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg
    63001 cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg
    63061 gcatcctgcc agccttcggc aatcgccaca ccaggcgctg ccggtaatgc acggattcgc
    63121 gtctggcgat attgtccaaa caaggcagtc aactgcgact gagaaagaat agctgccatc
    63181 tgagtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt
    63241 tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac
    63301 gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca
    63361 tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac
    63421 gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa
    63481 caacgtcgat catgatcggc aaggtaaacc gaacagacct cggtatccat cgcaagacag
    63541 atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg
    63601 actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa
    63661 gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac
    63721 tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac
    63781 cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt
    63841 tctgcatcgc cgctcaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc
    63901 ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt
    63961 gaagcaagga ttcgaacgtc tttacggctt aatcctactt cttcaaacaa ttcacggtac
    64021 atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc
    64081 tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg
    64141 tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg
    64201 aatgattgtt tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta
    64261 ataacaattg aataactcac agttatgtgc agagttataa acagaggaag gggtggatag
    64321 ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga
    64381 aaacccggag cattctgaat aagcccgata tagacatttt aaaatattca ttatcagtca
    64441 atgcattaca tgtaatttta gtaataaaaa agagtaattc gtgacccagg tcacacctct
    64501 catttacggg ttggcgaaag atcaaccaat gcggtattta tccacagaat gtgccactaa
    64561 gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatggaaa
    64621 cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc
    64681 tgtttatttt cagtgaccag atttggaaaa cccgttgcag tgttgcgcaa ctcgattaca
    64741 ggaagcctaa aaagcaatat aaatcagaga attgaacaac gcatgtggaa aaagttacac
    64801 tacgaatatt cggcacataa ttgctgttta ttttttaacc aaggtatcgt gacatgtccc
    64861 aacctcgccc actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc
    64921 aactttctgg ttatacattg ggagaactgg cggcacttgc cgggctggtt acaccagaga
    64981 atttaaaacg cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg
    65041 caggaagtaa acctgagcaa gattttgctg ccctgggcgt ggaacttaaa actatccctg
    65101 tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta
    65161 atagcggggt gacctgggaa accagccacg tacgccacaa gctcaaacgc gtactgtgga
    65221 taccggttga aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttac
    65281 tgtggagccc gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg
    65341 atatgattgt tctcggtcag gttgagcgga ttaccgctcg tcacggggaa tatttacaga
    65401 tacgaccgaa agcagcgaat gcgaaagcac ttaccgaagc cattggtgcc cggggcgaac
    65461 ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg
    65521 cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt
    65581 accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa
    65641 cgcctggctt gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat
    65701 tattttcctt tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg
    65761 tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcca tcgcctgggt
    65821 tacgcgcctg acgaatccgc tttttacaat attcagtcag gaaatttccg cccgtgattt
    65881 gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc
    65941 catcgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt
    66001 gcagattatg ctgctggata ttatcttcag cctcgattca gtgattaccg ctgtgggtct
    66061 gtcagatcac ctgtttatta tgatggcagc cgtggtaatt gccgtaggcg tgatgatgtt
    66121 cgctgcgcgt tcgattggtg attttgtcga gcgccatcct tcggtaaaaa tgctggcgct
    66181 ctctttcctg attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc
    66241 gaaaggttac atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaaccttat
    66301 tcgcaacaaa aagaacccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca
    66361 ctcctgttcc cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat
    66421 aaacagacta ttatcatagg tgggccaaat gaggataacc ggatgaaaaa atgggcagta
    66481 ataatttctg cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg
    66541 gcgaccaaag atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc
    66601 gggctggtga gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt
    66661 tcgcaaatta ttgaacgtta actaataagg tcagcatccg gctggcctta agatttttct
    66721 ctttcccttt tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg
    66781 aaacggctat gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc
    66841 ttggcacgat gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact
    66901 atgccgtcgc tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc
    66961 gccccgaaac gcaagggttg actgaaacct acgtcggcaa ctggctggcg aaacatggca
    67021 gccgcgaaaa gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg
    67081 gcatccgccc ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc
    67141 tcaagcgtct gcagactgat tacctcgatc tctatcaggt gcactggccg cagcgcccaa
    67201 ccaactgctt cggcaaactc ggttatagct ggacagattc tgcgcctgcg gtttcgctgc
    67261 tggatacgct ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg
    67321 tgtcgaacga aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc
    67381 tgccgcgtat tgtcaccatt cagaacccct acagcctgtt aaaccgcagt tttgaagtag
    67441 gtctggcaga agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt
    67501 tcggcacact gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc
    67561 tctttagtcg cttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg
    67621 ttgatatcgc cagacgtcat ggcctggatc ctgctcagat ggcgctcgct tttgtacgcc
    67681 gtcaaccgtt tgttgccagc actctgctgg gcgcaaccac gatggagcag ctgaaaacta
    67741 acgtcgaaag tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac
    67801 atcaggttta tacttatccg gcaccataag attaacgccg gttaaaaccg gcgttaaata
    67861 ttaatgacgg cgctgccaaa tccacagcgc cgttattgcc agcgcaaaca gcgcaccgaa
    67921 gccaatgcca atgggcacga ccgggatgcc taccattacc gccagcgagt aaatgcccag
    67981 catcaacaac atggcgctgt tttcgccaag gttttgtact gcaattgcat tccccgcccc
    68041 gacgcttttt ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc
    68101 cagcacgcca atcagcatca acaaggcata ggctggcaac agctcgtgtt gcagggagaa
    68161 aatcagtacc accacaccaa tcaaaatccc ggctggcata cagcgtgaca cggtttccag
    68221 cgtaactaac ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt
    68281 gagataggtc ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc acagcaccaa
    68341 caggaaacgc agcgtgacac ctgctcccca gaataaactg gtgcccacca gcgaaaaacg
    68401 cgtttcacca ttgcgccata gcgaggtgca ggcattcagg aaactgcggg tcatgttgat
    68461 gagattccag gactgccccg gacgcgccgc caccagtttg ggaatgtaga tattggcaac
    68521 gaccgcacca ccgtaggcca gtgcgcatgc ggccagggcg accaggacat gccagtcagc
    68581 cagcacacca ccggctacgg aaccgagcaa aatcgccgct atggtagaag cttccattaa
    68641 accgttagct ttcactaact tactacccgt ggttaattcg ccgagaatac cgtatttcgc
    68701 cggtgaatag gctgcagcgc caacacccac cagcgtatag ccgagaaacg gattgatacc
    68761 aaagcagata ctggctgcgc ccagcagctt caggccgttg gcaaacatca tcacccggcc
    68821 tttggcgaag ctatccgcca cctgtccgac aaacggcgca aaaagaatgt aagcacctac
    68881 aaacaccatt tgcaggatgg gctggctcca ctccggatag aactgcgctt tcagtaacgc
    68941 cagagtggca aacagcagcg cattatcgcc aaacgcagag agaaactgcg ccacgataac
    69001 cgctttcatc cccttcgacc acaacgaagt attagtgtgc actgactcac tcatcgtgtt
    69061 gttccgcttc gtctacccag cttttcaacg tgacaaagtc aggtttgccg ctgccaagta
    69121 atggcatctg tttcagatag cgaatatcgc gcggtacagc aagctccggc acgccgtgct
    69181 cgcgggcata ctgttgtaac ttatcgcgcg tcagttcgtt atctgtggtg aaaagcacca
    69241 gtgcctcgcc tttgctggca tcgctcttaa tcgcagtggc atggacttta tctggcgaaa
    69301 caccaagtgc cagttgttcc accatttcca gcgacaccat ttcgcctgca attttggcaa
    69361 agcgttttgc gcggccctga atctgcacaa agccctgctc gtcaaaacgc acaatatcgc
    69421 cagtgtcata ccagccgcgc tccatttcgc cgcgaacatt ctcggcggtg ggcacttcca
    69481 gtacacctgg cttctccacc cgcagatagc cgttcattat gttcggccct ttcagttgca
    69541 ggcgtccgcc ctcttcgata ccaggaaccg acaacaggcg cgcgtccata cctggtagaa
    69601 tacgccctac cgtaccgggt ttcgccgcca tcggtacgtt gatagaaacg acaggcgcgc
    69661 actcggtcac accgtagcct tcaaggatgc gcaggccaaa tttatcctgc caaagctgtt
    69721 tggtactttc ttgtagtttt tctgcgcctg ccaccacata gcgtagacga tagaagtcat
    69781 acgggttggc gaaacgcgcg tagtgaccga ggaaagtcga ggtaccgaac aacacggtgc
    69841 aactgcggtc atacaccaac tccggcacaa tgcggtaatg cagcgggctt ggataaagga
    69901 acacttctgc acctgtgagc agtggcgtaa acaggcctac cgtcagccca aaggagtgaa
    69961 acagcggtaa cgccgacata aagcgatcgt tggtggtgaa gtcggcaatc gttttaatct
    70021 gctcgacatt cgccagaatg cttttatggc tatggacgac gcctttcgga tggccttcag
    70081 aaccggaggt aaaaaggatc agcgcctctt cttccggctg ctgttttacc tgtgccagac
    70141 gcggcatcag caaatgagcg aagatccata ctttgtcggc agtggtgaca tctgctttta
    70201 aatcttccag atagacccag cgcacctgag taagttgctc cggcagatgc cagagtttgc
    70261 ctttatcgag aaactggcgg gaagtgaaga tggttttgat ttcagccgcc gtaatagcac
    70321 tggtcagccc ttttaccccg gcggtgtagt tcatcattgc gggaatgcgg cgacgggcga
    70381 tggccccaaa aatcactgcc gcactgatgc ctgcattggg cagcattaag ccgatgcgtt
    70441 cgccttcaac actgtatttt tcaaggatgc gtccaacaaa cagcgttttc gtaagcaatt
    70501 tgcgatagga gtctggggta aagttgacgt cttcgacaca tttcttcccg gctccgaagc
    70561 ggtatattgc actcagtaaa gattcgtaca gcgtttcacg cgggcgcacc gccatacgcg
    70621 cttccatcat tatttgatgc agcatttcgc cagcgatttt gcgacggtca cgggcacgcg
    70681 gcgcatccgg catctccacc tgcgttggtg gcaaaatatg cagagtaatt tgcgggaaca
    70741 agcgacgttt aaccagacct ttcaggcggc tgaagtgcgt aagttccgcc ccttcaatac
    70801 gcacaggaat aaccgttgca ccagacttcg ccgcgacaaa acccgcacca tcgtagattt
    70861 tcatcagcga gcctgtggtg gtgattcgtc cttcagggaa aatcaccact ggtcggccct
    70921 gttccaccag acgtaccaga tgtttaatcg ccataggttg cgtcgggtcg agaggaacaa
    70981 agtcgataaa tgatttcagc caacgcatat accactgttg gcttattgag gtgtaaacgg
    71041 caaataccgg acgtacgggt aaaaacaatg ccagcagtat gccatcaata aaagagacgt
    71101 gattaggcgt gattagaacg cgctcgccct tcagtgcctg ggtgtcaccc gtaacgcgaa
    71161 cgcgatacaa aacacggcac aaatttcgaa aaaagctaaa aagcattcca actccctttg
    71221 ctctgattca gtaaaagcga atggagggag attacacgag ataaagaacg cgagcgacag
    71281 taaattaggt gcgaaaaaaa acctgcgcat ccgcgcaggt tggtgcaaga gacagggtac
    71341 gaagagcgta ccgaataatc tcaccaatca atacctctgg gatcttgatt gtggtctgca
    71401 cgacgactct tcgccagtga gaaaacgcaa aggaatgaag ggaaatgcaa cgaggtgtgt
    71461 aaattgtcgg ttactgttac agattgatga ccggcaaaaa aaaacctgcg catctgcgca
    71521 ggctggtgta attcatgtgc tcaacccgaa gttgacttca cctatcaata cctctgggat
    71581 caccacttta gcaacctgaa gccaaacgcc accagcggtc aatcgcaaca gcgtttcgca
    71641 aagtgtaacc aaaggtttgc aatctctttt tttgtcttac ttatttgcgt ttggctcaca
    71701 ttcccacgat gaaaacacgc caccccttga accaacgggc gttttccgta acactgaaag
    71761 aatgtaagcg tttacccact aaggtatttt catggcgacc ataaaggatg tagcccgact
    71821 ggcaggcgtt tcagtcgcca ccgtttcccg cgtcattaat aattcaccca aagccagcga
    71881 agcttcccgg cttgctgtgc atagtgcaat ggagtctctt agctatcacc cgaacgccaa
    71941 cgcccgtgcg ctggcgcagc agaccactga aacggtcggt ctggtcgttg gtgatgtttc
    72001 cgatccgttt ttcggtgcaa tggtgaaagc ggtcgaacag gtggcttatc acactggtaa
    72061 ttttttattg attggcaacg gttaccacaa cgaacaaaaa gagcgtcagg ctattgagca
    72121 actgatccgc catcgctgtg ctgcgttggt cgtccatgcc aaaatgatac cggatgccga
    72181 tttagcctca ttaatgaaac aaatgcccgg tatggtgctg atcaaccgta tcctgcctgg
    72241 ctttgaaaac cgttgtattg ctctggacga tcgttacggt gcctggctgg caacgcgtca
    72301 tttaattcag caaggtcata cccgcattgg ttatctgtgt tctaaccact ctatttctga
    72361 cgccgaagat cgtctgcaag ggtattacga tgcccttgct gaaagtggta ttgcggccaa
    72421 tgaccggctg gtgacatttg gcgaaccaga cgaaagcggc ggcgaacagg caatgaccga
    72481 gcttttggga cgaggcagaa atttcactgc ggtagcctgt tataacgatt caatggcggc
    72541 gggtgcgatg ggcgttctca atgataatgg tattgatgta ccgggtgaga tttcgttaat
    72601 tggctttgat gatgtgctgg tgtcacgcta tgtgcgtccg cgcctgacca ccgtgcgtta
    72661 cccaatcgtg acgatggcga cgcaggctgc cgaactggct ttggcactgg cggataatcg
    72721 ccctctcccg gaaatcacta atgtctttag tccgacgctg gtacgtcgcc attcagtgtc
    72781 aactccgtcg ctggaggcaa gtcatcatgc aaccagcgac taaccgcagt taaagcaatt
    72841 ccagtgccag taattcttcg atggtctggc gacggcgaat taaccgcgcc tgaccattat
    72901 caaacagaac ttctggtaac agcggacggc tattgtagtt ggatgacatt gatgcgccat
    72961 atgcccccgt atcatgcaat accagataat cacccgcctt cacttccggc aaggcgcggg
    73021 tttcaacatt tcccccttcc tgctgggtaa agacatcgcc cgattcacat aacggcccgg
    73081 cgacgacggt ttccaccgtt ggtgcgtgtt ccagagaacg accatcagct gccagggcac
    73141 tgatatggtg gtaactaccg tacattgccg ggcgcatcag atcgttaaac ccggcatcaa
    73201 ccagcacaaa gtggcggcta cccatttgtt tgacgctccg cacctgagta attaacacgc
    73261 cagactgcgc taccaggaag cgacccggtt caatttccag tttcacaggg tggcccaaat
    73321 ggcgggcgat ttgctcacgc gcggcattcc acagaccata ataatgttcg gtatcaaccg
    73381 cctcttcacc ctgttgataa ggaatagaaa gcccaccacc cgcagaaata gcctgtaaat
    73441 cctgaccgaa ttcgaggacc agacgcacca tagcaccaca tacctgttcc agatgggcat
    73501 aatcaacgcc agaaccaatg tgcatgtgaa tgccgaccag ctgtagatga tgacgttgta
    73561 tcacgtccag tgcggcgggc agatcggtgt accagatacc gtgcttgctg ttttcgccac
    73621 cagtattggt tttttggcta tgcccgtgac caaaccccgg attaacacgc agccataccc
    73681 gatgccctgg cgaaacctga cccagttggt cgagcatatc aacagaaccc gcattcaccg
    73741 gaatttgcaa ttcactgacg cgttcaagcg tcgcctgatc gataacatct gccgtaaaaa
    73801 caatatcatc ggggtgcgtt tgcggattgt aacccgccgc caacgcacgc tctatttcgc
    73861 ctaacgagac ggaatccact ttcacgccct gctcacgcat taagcgcaaa atatgaatat
    73921 tggaacaggc tttctgtgcg aagcgcacca catcaaactg tttcagcgct gcaatctgcc
    73981 gacgaataat ttgcgcatcg taggcccaca ccgggcagcc aaattctgcg ggcaaacgca
    74041 gcagattttc ggcggtgagg tcggtatcgg tgctgaacag tgaatgtggc ataacaaact
    74101 ccagataagt gcttttttat gattacgcca catcataaaa agaataaaaa atatcgattt
    74161 atgttgagtc tatgcaaaaa tgatatggat taccggattg cgagagagcg ctaatggccg
    74221 ccgttaactt acgtcatatt gaaatttttc atgcggtaat gaccgccgga agcctgactg
    74281 aggcggcaca cctgctacac acctcacagc caacagtcag ccgcgaactg gcgcgctttg
    74341 agaaggtgat cgggctgaaa ttgtttgagc gcgtacgtgg acgattacat cctaccgtgc
    74401 aaggactgcg tctgtttgaa gaagtgcaac gatcctggta cggactggat cgcattgtca
    74461 gcgccgcaga aagtctgcgc gagtttcgcc agggagaact gtctatagcc tgcctgccgg
    74521 tcttttcgca atctttttta ccgcagctcc tgcaaccctt tctggcacgt tatcccgatg
    74581 tcagcttaaa tatcgtgccc caggaatcac ctctacttga agagtggctc tcggcccagc
    74641 gtcatgattt agggctcact gagacgctcc atacgcctgc gggaacagaa cgtaccgaat
    74701 tactctcttt agatgaagtg tgtgtgttac ctccgggtca tccgctggcg gcaaaagaga
    74761 tattaacgcc agaagatttt cacagtgaga actacatcag cctttcccgt actgacagct
    74821 atcgccagtt gctggatcaa ttgtttactg aacatcaggt taaacgacgc atgatcgtag
    74881 aaacccacag cgccgcgtca gtctgcgcaa tggtacgggc gggggtaggt gtttcggtgg
    74941 ttaacccgct caccgcactg gattatgcgg caagcggttt agtggtgcgg cggttcagta
    75001 ttgcggttcc gttcaccgtc agcctgatcc gccccctgca ccgcccgtca tcagcgctgg
    75061 ttcaggcgtt tagtgagcat ttacaagcgg gattaccgaa actggtcact tctcttgacg
    75121 ctattttgtc gtcagctacg acagcataaa agcgacagca tcctcggcat ggatcgccgc
    75181 ggtatcaaac acaggcagaa cactgcgctc ttctggcacc agtaaaccaa tttccgtaca
    75241 gccaaaaatg acgccctgtg cgccctgttc tgcaaggcga gcaatcactt gcgcataata
    75301 agtgcgtgac gcttcggtaa attgccccag acacagttct tcaaaaataa tctgattaat
    75361 tttcgcccgt tcatccgctt caggaatgag acagttgatg gaaaattgtt ccgtcagccg
    75421 cccgcgataa aaatcctgtt ccatggtgta acgcgtaccc agcagcgcca cacgagtcat
    75481 cccggccccg gtaattgcac gtccggtggc atccgcaatg tgtaagaaag gcagagagca
    75541 acgagactca atggcatccg ccactttgtg catcgtattg gtacataaca caatgccttc
    75601 tgcgcccgcc cgctgtaagc caagcgccgc ctcagccaaa atgtccccgg ttttgtccca
    75661 ctcgccgcga cgctggcact cttctatttc atgaaaatcg acgctatgta gcagcacttg
    75721 cgcagagtga agcccaccaa gccgctgttt aataccttca ttaatcagac gataataagg
    75781 aatagtggat tcccaactca ttcctcccag caaaccaatt gttttcattc ctctctcctg
    75841 atgtgtgtta ccgcaacaag gctgaataag gaacgagaca aacgcctcaa cggccaggtg
    75901 cccaatctct attaac
//
LOCUS       DAOTRK010000007        74140 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.007, whole genome shotgun sequence.
ACCESSION   DAOTRK010000007 DAOTRK010000000
VERSION     DAOTRK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.007"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..907
                     /locus_tag="QIE41_000497"
     CDS             <1..907
                     /locus_tag="QIE41_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDS3529425.1"
                     /translation="QPWVNALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDNLNRS
                     AEYIKEVFVSSGARVTAQDVPIAGGPYKNIVADYGPADGPLIIIGAHYDSVSSYENDQ
                     LTYTPGADDNASGVAGLLELARLLQQQAPKTGVQLVAYASEEPPFFRSDEMGSAVHAA
                     SLERPVRLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVRQV
                     KAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGD
                     TADRLNYQKMAQVVDGVITLLYNSK"
     gene            1010..1513
                     /locus_tag="QIE41_000498"
     CDS             1010..1513
                     /locus_tag="QIE41_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDS3529426.1"
                     /translation="MGMIGYFAEIDSDKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPANNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1581..3038)
                     /gene="nuoN"
                     /locus_tag="QIE41_000499"
     CDS             complement(1581..3038)
                     /gene="nuoN"
                     /locus_tag="QIE41_000499"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDS3529427.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3045..4574)
                     /gene="nuoM"
                     /locus_tag="QIE41_000500"
     CDS             complement(3045..4574)
                     /gene="nuoM"
                     /locus_tag="QIE41_000500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDS3529428.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4740..6581)
                     /gene="nuoL"
                     /locus_tag="QIE41_000501"
     CDS             complement(4740..6581)
                     /gene="nuoL"
                     /locus_tag="QIE41_000501"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDS3529429.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARSHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     ITHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6578..6880)
                     /gene="nuoK"
                     /locus_tag="QIE41_000502"
     CDS             complement(6578..6880)
                     /gene="nuoK"
                     /locus_tag="QIE41_000502"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDS3529430.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6877..7431)
                     /gene="nuoJ"
                     /locus_tag="QIE41_000503"
     CDS             complement(6877..7431)
                     /gene="nuoJ"
                     /locus_tag="QIE41_000503"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDS3529431.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7443..7985)
                     /gene="nuoI"
                     /locus_tag="QIE41_000504"
     CDS             complement(7443..7985)
                     /gene="nuoI"
                     /locus_tag="QIE41_000504"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDS3529432.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8000..8977)
                     /gene="nuoH"
                     /locus_tag="QIE41_000505"
     CDS             complement(8000..8977)
                     /gene="nuoH"
                     /locus_tag="QIE41_000505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDS3529433.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8974..11700)
                     /gene="nuoG"
                     /locus_tag="QIE41_000506"
     CDS             complement(8974..11700)
                     /gene="nuoG"
                     /locus_tag="QIE41_000506"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDS3529434.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11753..13090)
                     /gene="nuoF"
                     /locus_tag="QIE41_000507"
     CDS             complement(11753..13090)
                     /gene="nuoF"
                     /locus_tag="QIE41_000507"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDS3529435.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13087..13587)
                     /gene="nuoE"
                     /locus_tag="QIE41_000508"
     CDS             complement(13087..13587)
                     /gene="nuoE"
                     /locus_tag="QIE41_000508"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDS3529436.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13590..15392)
                     /gene="nuoC"
                     /locus_tag="QIE41_000509"
     CDS             complement(13590..15392)
                     /gene="nuoC"
                     /locus_tag="QIE41_000509"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDS3529437.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15486..16148)
                     /gene="nuoB"
                     /locus_tag="QIE41_000510"
     CDS             complement(15486..16148)
                     /gene="nuoB"
                     /locus_tag="QIE41_000510"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDS3529438.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16164..16607)
                     /gene="nuoA"
                     /locus_tag="QIE41_000511"
     CDS             complement(16164..16607)
                     /gene="nuoA"
                     /locus_tag="QIE41_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDS3529439.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17237..18175)
                     /gene="lrhA"
                     /locus_tag="QIE41_000512"
     CDS             complement(17237..18175)
                     /gene="lrhA"
                     /locus_tag="QIE41_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDS3529440.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19095..20312
                     /gene="alaA"
                     /locus_tag="QIE41_000513"
     CDS             19095..20312
                     /gene="alaA"
                     /locus_tag="QIE41_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDS3529441.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20396..20995
                     /gene="yfbR"
                     /locus_tag="QIE41_000514"
     CDS             20396..20995
                     /gene="yfbR"
                     /locus_tag="QIE41_000514"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDS3529442.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21054..22886)
                     /locus_tag="QIE41_000515"
     CDS             complement(21054..22886)
                     /locus_tag="QIE41_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDS3529443.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGEAMEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22973..23623)
                     /gene="hxpA"
                     /locus_tag="QIE41_000516"
     CDS             complement(22973..23623)
                     /gene="hxpA"
                     /locus_tag="QIE41_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDS3529444.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKVAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23634..24128)
                     /locus_tag="QIE41_000517"
     CDS             complement(23634..24128)
                     /locus_tag="QIE41_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDS3529445.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24211..24666)
                     /locus_tag="QIE41_000518"
     CDS             complement(24211..24666)
                     /locus_tag="QIE41_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDS3529446.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25004..26206
                     /gene="ackA"
                     /locus_tag="QIE41_000519"
     CDS             25004..26206
                     /gene="ackA"
                     /locus_tag="QIE41_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDS3529447.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26281..28425
                     /gene="pta"
                     /locus_tag="QIE41_000520"
     CDS             26281..28425
                     /gene="pta"
                     /locus_tag="QIE41_000520"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDS3529448.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28615..30135
                     /gene="yfcC"
                     /locus_tag="QIE41_000521"
     CDS             28615..30135
                     /gene="yfcC"
                     /locus_tag="QIE41_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDS3529449.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30168..30710)
                     /gene="yfcD"
                     /locus_tag="QIE41_000522"
     CDS             complement(30168..30710)
                     /gene="yfcD"
                     /locus_tag="QIE41_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDS3529450.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30768..31319)
                     /gene="yfcE"
                     /locus_tag="QIE41_000523"
     CDS             complement(30768..31319)
                     /gene="yfcE"
                     /locus_tag="QIE41_000523"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDS3529451.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31375..32019)
                     /gene="yfcF"
                     /locus_tag="QIE41_000524"
     CDS             complement(31375..32019)
                     /gene="yfcF"
                     /locus_tag="QIE41_000524"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDS3529452.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32155..32802
                     /gene="yfcG"
                     /locus_tag="QIE41_000525"
     CDS             32155..32802
                     /gene="yfcG"
                     /locus_tag="QIE41_000525"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDS3529453.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32859..33221
                     /gene="folX"
                     /locus_tag="QIE41_000526"
     CDS             32859..33221
                     /gene="folX"
                     /locus_tag="QIE41_000526"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDS3529454.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33242..34135
                     /locus_tag="QIE41_000527"
     CDS             33242..34135
                     /locus_tag="QIE41_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDS3529455.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSCRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34183..35073)
                     /gene="rpnB"
                     /locus_tag="QIE41_000528"
     CDS             complement(34183..35073)
                     /gene="rpnB"
                     /locus_tag="QIE41_000528"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDS3529456.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLERGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35270..36043)
                     /gene="hisP"
                     /locus_tag="QIE41_000529"
     CDS             complement(35270..36043)
                     /gene="hisP"
                     /locus_tag="QIE41_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDS3529457.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36051..36767)
                     /locus_tag="QIE41_000530"
     CDS             complement(36051..36767)
                     /locus_tag="QIE41_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3529458.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVMALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36764..37450)
                     /gene="hisQ"
                     /locus_tag="QIE41_000531"
     CDS             complement(36764..37450)
                     /gene="hisQ"
                     /locus_tag="QIE41_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDS3529459.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37540..38322)
                     /gene="hisJ"
                     /locus_tag="QIE41_000532"
     CDS             complement(37540..38322)
                     /gene="hisJ"
                     /locus_tag="QIE41_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDS3529460.1"
                     /translation="MKKLVLSLSLVLAFSSATVAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38543..39325)
                     /gene="argT"
                     /locus_tag="QIE41_000533"
     CDS             complement(38543..39325)
                     /gene="argT"
                     /locus_tag="QIE41_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDS3529461.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39591..40160)
                     /gene="ubiX"
                     /locus_tag="QIE41_000534"
     CDS             complement(39591..40160)
                     /gene="ubiX"
                     /locus_tag="QIE41_000534"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDS3529462.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADVETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40255..41772)
                     /gene="purF"
                     /locus_tag="QIE41_000535"
     CDS             complement(40255..41772)
                     /gene="purF"
                     /locus_tag="QIE41_000535"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDS3529463.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41809..42297)
                     /gene="cvpA"
                     /locus_tag="QIE41_000536"
     CDS             complement(41809..42297)
                     /gene="cvpA"
                     /locus_tag="QIE41_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDS3529464.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42465..43127)
                     /gene="dedD"
                     /locus_tag="QIE41_000537"
     CDS             complement(42465..43127)
                     /gene="dedD"
                     /locus_tag="QIE41_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDS3529465.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43117..44385)
                     /gene="folC"
                     /locus_tag="QIE41_000538"
     CDS             complement(43117..44385)
                     /gene="folC"
                     /locus_tag="QIE41_000538"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDS3529466.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44455..45369)
                     /gene="accD"
                     /locus_tag="QIE41_000539"
     CDS             complement(44455..45369)
                     /gene="accD"
                     /locus_tag="QIE41_000539"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDS3529467.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45525..46184)
                     /locus_tag="QIE41_000540"
     CDS             complement(45525..46184)
                     /locus_tag="QIE41_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDS3529468.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46267..47079)
                     /gene="truA"
                     /locus_tag="QIE41_000541"
     CDS             complement(46267..47079)
                     /gene="truA"
                     /locus_tag="QIE41_000541"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDS3529469.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47079..48092)
                     /locus_tag="QIE41_000542"
     CDS             complement(47079..48092)
                     /locus_tag="QIE41_000542"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDS3529470.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48158..49294)
                     /gene="pdxB"
                     /locus_tag="QIE41_000543"
     CDS             complement(48158..49294)
                     /gene="pdxB"
                     /locus_tag="QIE41_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDS3529471.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGRPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49393..50388
                     /gene="flk"
                     /locus_tag="QIE41_000544"
     CDS             49393..50388
                     /gene="flk"
                     /locus_tag="QIE41_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDS3529472.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKNILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50385..51563)
                     /locus_tag="QIE41_000545"
     CDS             complement(50385..51563)
                     /locus_tag="QIE41_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HDS3529473.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51829..53049)
                     /gene="fabB"
                     /locus_tag="QIE41_000546"
     CDS             complement(51829..53049)
                     /gene="fabB"
                     /locus_tag="QIE41_000546"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDS3529474.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53208..55214
                     /gene="mnmC"
                     /locus_tag="QIE41_000547"
     CDS             53208..55214
                     /gene="mnmC"
                     /locus_tag="QIE41_000547"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDS3529475.1"
                     /translation="MKHNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFVQFREAHPQAQLQRLHFISFEK
                     FPLTRTDLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSKLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFEHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLELAQQQGLQIHYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHNVVVLANGHQISGFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55335..55613)
                     /locus_tag="QIE41_000548"
     CDS             complement(55335..55613)
                     /locus_tag="QIE41_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDS3529476.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55647..56195)
                     /gene="epmC"
                     /locus_tag="QIE41_000549"
     CDS             complement(55647..56195)
                     /gene="epmC"
                     /locus_tag="QIE41_000549"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDS3529477.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLENGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56195..57004)
                     /locus_tag="QIE41_000550"
     CDS             complement(56195..57004)
                     /locus_tag="QIE41_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDS3529478.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57004..57828)
                     /gene="mepA"
                     /locus_tag="QIE41_000551"
     CDS             complement(57004..57828)
                     /gene="mepA"
                     /locus_tag="QIE41_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDS3529479.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGLGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLKCEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57832..58917)
                     /gene="aroC"
                     /locus_tag="QIE41_000552"
     CDS             complement(57832..58917)
                     /gene="aroC"
                     /locus_tag="QIE41_000552"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDS3529480.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFEVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58952..59884)
                     /gene="prmB"
                     /locus_tag="QIE41_000553"
     CDS             complement(58952..59884)
                     /gene="prmB"
                     /locus_tag="QIE41_000553"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDS3529481.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60050..60601
                     /gene="smrB"
                     /locus_tag="QIE41_000554"
     CDS             60050..60601
                     /gene="smrB"
                     /locus_tag="QIE41_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDS3529482.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTQLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPELP"
     gene            complement(60725..61594)
                     /locus_tag="QIE41_000555"
     CDS             complement(60725..61594)
                     /locus_tag="QIE41_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDS3529483.1"
                     /translation="MSIYNMKKWTLFFASLMLLVLNVVGTPRSYAGDKLMSSSFDSVR
                     VYYAMDTVTGVAASIVFNVTVLTPGEVAYGKYNSIGRKGDTLRLISWTGSGPAPTLVI
                     TDYDTINNSHCPGIDTDLFRCAYMTFKVTVASDNYGCPWIASFYSYTNLPGYFDGSYT
                     SPTVHNSICPTIPVASYDISWSENYVSHNKALQLQSTGSTITTTLSTYLMENGKLCDG
                     SVFDSRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKELHDIVVKVNTSSRQPI
                     DSTCRFQYVLNEL"
     gene            complement(61581..62105)
                     /locus_tag="QIE41_000556"
     CDS             complement(61581..62105)
                     /locus_tag="QIE41_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3529484.1"
                     /translation="MKKKCALIFINSLLLLNSGTTTAVDNLHFTGNLISKSCTPVING
                     SQLAEVHFTAIAASDLMNIGQSERVPLVIQLKDCQSSTSLSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGIPVPVNNTTGVTLPLTQGNNSLNFNAWLQAKSGRNVTS
                     GDFNATVTATFEYL"
     gene            complement(62102..62572)
                     /locus_tag="QIE41_000557"
     CDS             complement(62102..62572)
                     /locus_tag="QIE41_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3529485.1"
                     /translation="MKRISLLVLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMTLSWSGTQSDFDDAAVSSNITGLG
                     IQLKQAGQRFRINTPLVVNETDLPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            complement(62569..63117)
                     /locus_tag="QIE41_000558"
     CDS             complement(62569..63117)
                     /locus_tag="QIE41_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3529486.1"
                     /translation="MKSRAAKANDGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKIDGVSYNRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHSILDLTSGSWLPFNFSSGIPALEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63092..63841)
                     /locus_tag="QIE41_000559"
     CDS             complement(63092..63841)
                     /locus_tag="QIE41_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDS3529487.1"
                     /translation="MTKYLSAKTLTTVLSLLAATVSFSAQASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKLPYLAQSWIEDEKGVKISSPLAVLPPVQRIEAMMNGQVKIQGLPDIH
                     KLPADRESMFYFNVREIPPKSNKSNTLQIALQTRIKLFWRPKALEKNTTQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPVL
                     TYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(63861..66500)
                     /locus_tag="QIE41_000560"
     CDS             complement(63861..66500)
                     /locus_tag="QIE41_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDS3529488.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNALADDTIQFDGRFLDLKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNQPLPDDYDIYWYATENDPNKSYACLSPELV
                     AQFGLKEDIAKNLQWIRDGQCLNTALLAGTEISGDLGQSALLVSVPQAYLEYTDSEWD
                     PPSRWDDGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNLRGYAPDISGVAHTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALLGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDDDRARTGNDKEMY
                     TVTYNQNFTDARVSVYLNYSHHTYWDRQDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSNQVSLYHRIDDASHYTLSAGTS
                     ENHSSVDGYYSHDGTLAKVDLSANYHEGQYTSAGISLQGGATLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATK
                     SVVQATLTEGAIGYRKFAVISGEKAMAVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSSPVIPHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            complement(66584..67150)
                     /locus_tag="QIE41_000561"
     CDS             complement(66584..67150)
                     /locus_tag="QIE41_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3529489.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMATTFTGNPYSTN
                     ADNYSLSNMDNGTEIPNVSLVIGDQHGTGYALGAEIKQPIVKDSSTGKGKPKQTLNFK
                     AWLVGETDAVTPTPAPFETLTTFQITYL"
     gene            complement(67812..68297)
                     /gene="sixA"
                     /locus_tag="QIE41_000562"
     CDS             complement(67812..68297)
                     /gene="sixA"
                     /locus_tag="QIE41_000562"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDS3529490.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68500..70644)
                     /gene="fadJ"
                     /locus_tag="QIE41_000563"
     CDS             complement(68500..70644)
                     /gene="fadJ"
                     /locus_tag="QIE41_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDS3529491.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTAIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(70644..71954)
                     /gene="fadI"
                     /locus_tag="QIE41_000564"
     CDS             complement(70644..71954)
                     /gene="fadI"
                     /locus_tag="QIE41_000564"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDS3529492.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(72135..72419)
                     /locus_tag="QIE41_000565"
     CDS             complement(72135..72419)
                     /locus_tag="QIE41_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDS3529493.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            72791..74131
                     /gene="fadL"
                     /locus_tag="QIE41_000566"
     CDS             72791..74131
                     /gene="fadL"
                     /locus_tag="QIE41_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDS3529494.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSDLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPMLSQQLIARGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGPAAYSFESEGKAWLFGTNFNYAF"
BASE COUNT        18450 a        19987 c        18440 g        17263 t
ORIGIN      
        1 ccaaccctgg gtcaatgcat taccgccaac gccccgacac gcaagtcctg agcaattaga
       61 aaaaacagtt cgttatctta cacaaactgt gcatccacgt agtgccgaca atattgataa
      121 tctgaatagg tccgcagagt atataaaaga ggtctttgtc agtagcggtg ccagagttac
      181 cgcgcaggac gtccccattg cgggtggccc ctacaaaaac attgttgctg attatggtcc
      241 tgccgatgga ccgctgatta ttattggtgc gcactatgac tctgtcagta gttatgaaaa
      301 tgatcaattg acctatacgc cgggcgcgga tgataacgcc agcggcgtgg caggattact
      361 cgaactggca cgtttgttac agcagcaagc accgaaaact ggcgtgcagc tggtcgccta
      421 tgcgtcggaa gaaccgccct tctttcgtag cgatgaaatg gggagcgcgg tacatgcagc
      481 ttcgcttgag cgtccagtga gattaatgat agcgctggag atgattggct attacgactc
      541 tgcgcctggt agtcaggatt acccttaccc ggcaatgtcc tggctttatc ccgatcgggg
      601 agactttatt gccgtggtcg gcagaatgca ggatatcaac gccgttcgtc aggtaaaagc
      661 ggcattgttg tcatcccggg atttatctgt ttattctatg aatgccccag ggtttattcc
      721 cggtattgat ttctctgacc acctgaatta ttggcaacac gatattcccg cagtaatgat
      781 tactgacacc gctttttatc gtaataaaca ataccacttg cccggtgata ccgcagacag
      841 attgaattat cagaaaatgg ctcaggtagt ggatggggtt ataactttgt tatacaacag
      901 taaataataa aaatttctcg aggcgggtcc agaaaaagcg acatgtacat tttattttcc
      961 ctcgtctgtt ttaagatgcg aaacatgaaa ttcaacggag gaaaataaaa tgggaatgat
     1021 tggctatttt gcagaaattg actcagataa gattaaccaa ctgcttgagt ctactgaaaa
     1081 acccttaatg gacaatattc atgacacgct ttcaggatta cgccgactgg atattgataa
     1141 acgctgggat tttttacact ttgggctgac aggcacctct gcttttgatc ccgcgaataa
     1201 tgatccgctg agtcgtgccg tgcttggtga acacagcctc gaagatggca tagatggctt
     1261 cctgggattg acctggaatc aggagttagc cgcgacaatc gaccgactgg aagctctgga
     1321 tcgaagtgag ctgcgtaaac agttttccat taaacgactc aatgaaatgg agatttaccc
     1381 tggtgtgact ttttcagaag agttagaggg gcaactcttt gcttcgatca tgcttgatat
     1441 ggaaaagctg attagtgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt
     1501 tattgttggg tgatggggtc agggcaccaa aaaaaaaccg ccgaacatgt cggcggcttt
     1561 ctgacttaca aactgactgc ttacatcagc ggcatggcca accgcacaat gctaatcagc
     1621 ggttgtggcc atacacccag caccagtacc aacagtgcag agatcagcac cacgataccg
     1681 cccgcgctgt actgccagtt tgaaggtgca tcgcgacccg gttgttccgg ggcgtgaaga
     1741 tacaagctca ccgccacgcg caggtagtag tagaggccga ttgccgaacc gacaaccacg
     1801 gcacccacca gccaccacaa gtgtgcctgg acaccgaccg ccagcacgta gaacttacca
     1861 ataaagccca gcgtcatcgg aataccggcc aaagacagca tcatcaccgt catcaccgcc
     1921 gcgaggatcg ggcgatgcca gaacagaccg cggtaagaga acagggaatc agcatccggg
     1981 ccacgatacg ggctggacat caggctgacc acgccgaacg cgccgaggct gctgaacaga
     2041 taaccggcca ggtaaacccc taccgcttcc atcgacatct cgccggtttg cagcgcaatc
     2101 agcgccacca gcagatagcc gaggtgagag atagatgagt aaccgagcaa acgtttgata
     2161 ttggtctggc tcagcgccat caggttaccg aagatgatgg aggcaaaggc gataatcgcc
     2221 agcaccaccc gaatcgcttc gctgtcaccc accggtgcgt agaggaacag acgcatcacc
     2281 acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg cgcaggcgcg
     2341 ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt gaagccgagg
     2401 ccaacaatca tcaggccgaa acctgccagc aacagcggct cgttgagcat accgtcgccg
     2461 aggtttttac ccagcgcgac aaacgacagg tcgccagact gcgcatacac cagcgccata
     2521 ccgaacagca ggaaagaaga cgctgcggca gagagaatgg tgtatttgat acttgcttcc
     2581 agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa agagatcagt
     2641 tcaataccga ggaacaaaga cgccagatgg ttggcattcg ccagcaggat cccgcccagc
     2701 gcggcaatta acaccaacag gtagaactcg tccttgttgt cgttatagcc ttcaagccac
     2761 gggtaggcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt gtaaagcatg
     2821 gcgaaaccat caacgcgcat cagtggcgta acgtccatag cgcccgcctg gccgacaaac
     2881 cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt gaggaaatga
     2941 ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac gatcagcaac
     3001 ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg ccttgtagta
     3061 gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga ggtatccaga
     3121 atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag gatcataaac
     3181 agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt cgctttaccg
     3241 aagtaagcgc gatgtaacat cgccagcgaa taaacagagg caaagaccag cccaaaggta
     3301 gagataacgg taatcaccgg gacaacctgg aagctgccga acagaatcat aaattcgccg
     3361 acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag cgacagtgct
     3421 ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg ggtatggata
     3481 cgttcataaa gctgaccgca gagaataaac agacccgccg ctgacaagcc gtgcgcaatc
     3541 atctggatta ccgcgccctg gtaggccaac tggctgccag tgtagatagc aatcagcacg
     3601 aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt ctgggcgaag
     3661 gccatccacg caccgtagaa gatgccgata acacccagcc acatggcgat tggcgcgaac
     3721 tctgccgacg cgttcgggaa cagcggtagg gagaaacgca gcagaccgta agcggcggtt
     3781 ttcagcaaga tccccgcgag gtccacggaa ccggcggttg gagcctgtga gtgcgcatcc
     3841 ggcagccagc catgcagcgg aaccaccggc attttcaccg caaatgcgat gaagaagccc
     3901 agcatcaaca gatattccac accactggac attggcgtat tcaacagctc ttcatagttg
     3961 aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat ggcgatcaac
     4021 atcaccagac cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt gatacgcgtt
     4081 ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg caccagcatc
     4141 atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc gataacgccg
     4201 cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat ctctttccac
     4261 gaacagagta ccgccagcac accgagcaga ccagtcagca cgaccatcag cagcgatagc
     4321 ccgtcaatgg cgagatggat agagatacca aaacgcggga tccacggcat gtcgaattca
     4381 gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg caaccacagt
     4441 tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg cggcaccttg
     4501 acgccaaagc gttcggtctg ccagcacagg aagccgccga taaagggaat taatattagc
     4561 cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac cggattttca
     4621 acgaattctc acgacagaaa attctcttcg gattgtggtt gtagtgtcgg atgcgccgcg
     4681 aaggcggctt atccgaccta cgttcttata tgcccagggg caagcccaca atccttaact
     4741 caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat ggatgccaca
     4801 taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa gcgggaaagg
     4861 acagccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca ggcaataccc
     4921 aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc gttgtaccac
     4981 caggtgccca gcagacggcc cggcgcactg ttggcgatgg aagtcaccag agtacgttta
     5041 cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc agaggtaatt
     5101 tccagggtca acatgctgcc gtgcgccagc tccgtcgttt gcgggagcac gccctgcagc
     5161 ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat cagcggcagg
     5221 ctgtgagtta tccctttcac ggcgtgagcg tgaatttgtt cttttccgtg gaagacgatg
     5281 aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc agccaccatc
     5341 agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact gaagaagccc
     5401 gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca gagataaacc
     5461 agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg atggcaggcc
     5521 agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg agtcatcaag
     5581 tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta gccaatctgg
     5641 ctcatggtag agtaagcgag aacacgtttg atatcggtct gtaccagcgc ggcaaaaccg
     5701 gccagcagca gcgtaaccgc cccaacaatg cccaccagat gcagaacttc cggcgtcatc
     5761 aggaacaggc cgtggctacg ggcgatcagg tagacacccg cggttaccat ggttgcggcg
     5821 tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cggcaagcca tgtctgcaac
     5881 ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc ccacatcagc
     5941 atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg gaagttcagg
     6001 gtgcccagtt cgttgtaaag aatgaacagc gcgaaagcga ggaagacgtc acccacacgg
     6061 gtcacgacga atgccttcat tgccgctgcg ccattcttcg gatcggtgta atagaaaccg
     6121 atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag cagcaggttg
     6181 tcggcaagca ccagaactac catgctggcg atgaacaggt tggtgtaagc gaagaagcga
     6241 gagtagccct cttcaccgcg catataccag gaggcgtaca tgtgaataag gaaacccaca
     6301 ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt aaaaccgatg
     6361 ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg ctcaccgtta
     6421 gcgaagaagt caacgccgat aaaggcagtt accagcgccg ccaggcccac agagccaacg
     6481 ccgacgatcg ccgagacgtt ttcagaccag cgcccacggg agaacgccag caggacgaag
     6541 ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc tcacttactg
     6601 aatcaatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgct aggccgatac
     6661 tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg tcggtctggc
     6721 cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg atttccagac
     6781 caatcagcat aaacagcaga ttgcgacgga taaccagacc agttaagcca agaatgaata
     6841 agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct ccgtttttct
     6901 tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt cacgaccgac
     6961 gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca ccgccagtac
     7021 gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac cgtcgatacc
     7081 ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg ccgacaaaat
     7141 tgccggacca atccacacct gcggtttcag ccactggcgt tcctgttcaa tttctgaacc
     7201 gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac ccgcgtagac
     7261 gataatttcc agcgcaccgg cgaagtaagc gcccagtgag aagaacaccc cagagatcgc
     7321 cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg tgatcactcg
     7381 caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca ttgcccctct
     7441 ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc ttcgccctta
     7501 tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg gtatttgccc
     7561 ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt gtattccccc
     7621 atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc gcacagacca
     7681 cagaaaatgc agcgtgagaa gttgatgcgg aaaaattccg gataccagcg accgtctttg
     7741 gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcgca gaggttacag
     7801 gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc acgataacgg
     7861 ggcggcagat agaccggttc ttccgggtac attcgcgttt cgcgtttggc gaacgcgtgc
     7921 aggccgatca tccagatact acgaacctgg gtgccgaatc ctactaacaa ttcttttaag
     7981 gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc gttaccagca
     8041 agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc tggtcataac
     8101 gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac gcggttttca
     8161 gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag aacagcgtca
     8221 ccatcagtgc agagatggtc acaatcccga tgtattcgcc cacgaagaac agaccgaact
     8281 tcatgccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct tccggctggt
     8341 caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata aaaccaaaga
     8401 attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc atgttgaatg
     8461 aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg tagctcagag
     8521 tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt gaccagcccg
     8581 caaacagcac cgcgtagacc gccagaccgg ccatcatcag gaagaacaaa atcccgatgt
     8641 tcaggtcggc aaccacccaa cctgggctga ctggcacaat cgcaaaggcc agcagcagcg
     8701 aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat ttcgggatcc
     8761 agtcttcttt aaagaacatc ttgatcatgt ccgcgaccag ctggagcgaa ccgccccagc
     8821 caacacggtt aggtccgtaa cggttctgga acagacccag caggcgacgt tcgccaaagc
     8881 tcatgaatgc cccacaggtg acaaccacca gcaggatcac caccgctttg aggatggtca
     8941 gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct tgagatcctc
     9001 aagatgcgcg cccgccagca ccggagcaat gccggacata cccatcggca agcccacctg
     9061 ccctgccgtc agcccttcgg cgatttcaac cggcagcgtg accgtgttgc catcgtaact
     9121 aaaggagacg cgtgtacccg cgttcacacc caacttcgcg gcatccgctg ggttgagttt
     9181 gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca attcatcgct
     9241 gccaaacagg tggtaatacg gcgcgatacg ccatttcccg tcctgcggct ggaagcgtgc
     9301 cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac gcacgcccgg
     9361 atcgccaaag cgcagtttgc cgccaacttc gtcctggaat ttgttccacg cctgcgggga
     9421 gttccagcct ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg gctggttgtt
     9481 accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg gctcatgaac
     9541 gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac gggccagttt
     9601 ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg ccagttccgg
     9661 gattttcgcc acaacagcgt caatcacatg gtcgagctgc gtccagtcca cttcacggct
     9721 cagcaaggtg ctatgcagcg agtgtaacca gcgccagctt tccagcatga cagttttgct
     9781 gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt cattattaat
     9841 taccgtaccg tcgctttcag caaagctggc agcagaaagt accagatggg cgttttccat
     9901 aatcgctgtg cgttgatggt caaccaccat caccagcggc gctttagcca gcgcagcatt
     9961 cacgcgggta gcggaagcgt gacgatgcag atcgttttcc agcaccacca ccgcgtcggc
    10021 gcgtccggtt tccagttcgg tcaacgcttc ttcaagcgaa ccgcccccca taatgcccag
    10081 ccccatgctg tttacagagc gagcaatcat ggtgatacca acgtcagcgc cgcgaccttt
    10141 cagggctttc gcgacgttag ccgccgcctg aatcacctct aagctaccgg cgttcgtccc
    10201 ggagataatc aacggtttct tcgcaccagc cagtgcctgc acgatgacgt cgattttgct
    10261 ttgcagctca ggttcaatac cgtcaaccgc tggtgcggag ttgtccagcg catgggcgat
    10321 ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg ccgcgatatc
    10381 atccagacgg gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac gttgaccgat
    10441 gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgccgcca tttcgcgcgc
    10501 tttacctttc accgcctgac gaactgccag cgcgacgcgc gcgccggtct gggtaacgtc
    10561 ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagtgccg gcgtataaat
    10621 gccgccttca cgtagcactt tcagcgccag ttgcagacgt tcctgctcac cgtgagcgat
    10681 accggtatag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc tttctacgct
    10741 ggcacgcgga gaaccgatgc cgatcacttt cttcgactgg cgcagaatat ctgctgcgcc
    10801 ctgcattgcc tgttcggcat tgagggtaat gaaatcatcg ccacgacgct gtactggctg
    10861 acgcggacga tctttcaggt tgacgtaacc gtaaccgaaa cgaccacggt cgcagaggaa
    10921 gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc cataacgctc
    10981 gccggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg caaactgcat
    11041 atcccattta cggttgtaac gctcggagtg cgttttgtcg gtgaatacgc cggtcgggca
    11101 aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg ggcgaccgaa
    11161 gtagacgttg tcgtgcgcac cgtaaacgcc caggtccgtg ccgtcagcgt aatctttgta
    11221 gtaacgcacg cagcggtagc aggcgatgca gcggttcatt tcgtgagaga tgaatggccc
    11281 caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc tgtgtccggt
    11341 catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg gacagtcgtg
    11401 cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg cttcttcatc
    11461 gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca ccaggcgacc
    11521 acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag caccgacgct
    11581 tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca gacaagcttc
    11641 cagcaagttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa ttgtagccat
    11701 tagcatgctt ccagttttct cagtcagaga ctgagcgtta atcgaaattc ggttaccagc
    11761 gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg aacggctgtt
    11821 tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac ggctccactg
    11881 caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac agttgctcaa
    11941 gtgtttcgat atcgcccggc tgaccttcgc cacgctccag cgcacgcaga attttcacgc
    12001 tccacggcag accgtcgcgg cacggcgtac accagccaca ggactcacgg gcgaaaaact
    12061 cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc atcgccagcg
    12121 ccgtacccag acggcttccc gctttaccga tactttcgaa ttccatcggc agatcaaggt
    12181 gcgcttcggt caggaagtcg gtgccagcgc cgcctggctg ccaggctttg aatttcagac
    12241 cgtcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg ccgaatggca
    12301 gttcccacag gcccggattt ttcacccgac cggagaagcc catcagcttg gtgccagcat
    12361 ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc gccggaacgt
    12421 tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg gaggttgccg
    12481 ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg attaacgcgg
    12541 tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg aagtcaaaac
    12601 ctgttcccat aatatttttg ccaagcagac ctgcttcggt ggcttcggca atggcacggc
    12661 gcagattcac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca cggtaagctt
    12721 tcagcgcaaa cgcggagata agcatacctt ccaccagcag gtgcggcagt tgctccatca
    12781 gcaggcggtc tttataggtg cccggctcca tttcatcggc gttacatagc aggtaacgga
    12841 tgttcatgga ttcgtctttc ggcatcaggc tccatttcag gccagtcgaa aagcccgcgc
    12901 cgccgcgccc tttcagacca gcgtctttta cctgattaac aatttcgtcc ggagacagcc
    12961 cggtcagcgc cttacgcgcg ccttcgtaac cgtttttgct gcggtattca tccagccaca
    13021 ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga gtacggataa
    13081 tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca gatgcgcgtg
    13141 agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc agcaagttgg
    13201 cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca gctttttctc
    13261 gagcgccgcc tgaataccct gataaccgtt gatatgacag accacgctgt cgcaataacg
    13321 gatcacatgg cgaccaaccg gctggcggaa gatctggcta taaaacgttg ccacaccttc
    13381 gacgtcgctc gccggaatac ccagcacatc ggcgatcgcg tggatcgcgc catccggcac
    13441 ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac gcgggtcttc
    13501 gtagtggtgc atttcgtgct caatcgcttc acgctctgcc gcactcagct caaaagcctc
    13561 ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat aacaaaatcg
    13621 atactgccca gataaacaat caggtcagac accaggctgc cgcggatcgc cgccggaatt
    13681 tgctgcaaat gcgcataact cggcgtacgg atacgggtgc gataactcat ggtgctaccg
    13741 tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg gaaagattca
    13801 ttggctggca tcaccggacc ccacgacact tgcaggaagt gggtgatcag ggtttcgata
    13861 tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc tttgaacggg
    13921 ccttccggca tgttgttgag gcactgctca agaatgcgca gactctgacg cagctcttcc
    13981 actttaagca ttacgcgggt gtagcagtca gaaacaccac cacccaccgg gatttcaaag
    14041 tcgaagtttt catagccaga atacggacgc gccttacgca cgtcgaagtc gatcccggta
    14101 gcacgcaggc ccgcgccggt ggtgccccac tccagcgcct ctttcgcgcc ataggcggca
    14161 acgccctggg aacgaccttt cagaatggtg ttttgcagcg ccgctttctc gtaagacgcc
    14221 agacgtttcg gcatccagtc gaggaactca cgcagcaggc gatcccagcc acgcggcagg
    14281 tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc cgtgatcgct
    14341 tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacactgg cgtcattgcg
    14401 ccgacgtcct gaataaaggt cgagatatac agcagatggc tgttgatgcg gaacagctca
    14461 gaaagcatta cgcgaatgac gttaacgcga tccggcacgg tgatcccggc cagtttctct
    14521 acagccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcgat acggtcggta
    14581 tacggaatgt agctatgcca ggactggcgc tcgcccattt tctccgcacc acggtggtgg
    14641 tagccgatgt ctggtacgca gtcgacaatc tcttcgccat cgagctgcaa aacgatacgg
    14701 aaagccccgt gcgccgacgg gtggttcggg ccgaggttga ggaacatgaa gtcctcgttt
    14761 tcggttccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc catctccaga
    14821 tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc cgggtaatct
    14881 ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag gttcgggtga
    14941 ccgtcgaaag taatgccaaa cagatcccag gtctcacgct cataccagtt agcgttcggg
    15001 aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac cttcaacatg
    15061 atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc cgcggcaggt
    15121 aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa cagcatgacg
    15181 taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc acgcttgatc
    15241 cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg cccaaaacgg
    15301 ttgcgcagtt cgccaatcac cggatcatca agatgatcgc gggtctgcca ggcgggttct
    15361 tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg actgtcggcg
    15421 cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt gccgacaggc
    15481 gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc accgcgcttg
    15541 cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac cacccaggag
    15601 agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg catgtacgct
    15661 tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt atcaacgccc
    15721 tgcacaacgg aataaatatc gtacataccg ccagagttgg cacaggcacc cattgagata
    15781 acccattttg gctccagcat ctggtcatac agacgctgaa taaccggtgc cattttggta
    15841 aagcaggttc ctgccaccac catcaggtca gcctgacgcg gcgaagcacg caatacttct
    15901 gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc aacgtaacag
    15961 caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt aaccatgtca
    16021 ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc ggttacgatc
    16081 tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg ggtgagcgta
    16141 taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt cgtttccggg
    16201 ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat acgcaccaga
    16261 taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa gcctacccag
    16321 ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac gtcgaagata
    16381 acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc ggagccgacc
    16441 gagtcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg accgcctaaa
    16501 aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag aaagatagcg
    16561 aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc ttactcatca
    16621 aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga ttcatgggga
    16681 ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa ttgatgtggt
    16741 cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca cattagttta
    16801 cataatatca acaccattag catttaatgc cctttcaccc cagatccttg acgactccag
    16861 gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat acgggtctat
    16921 tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt gatctgtgac
    16981 acgcttttgt cattccataa caaaaacgca gcaacaaatt tacgtatttt ttaacatcat
    17041 tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa tctctggtga
    17101 ttgaaaaatg aacagttttg tatgttctgc acgatacgag gaaggctttt tactaaaaaa
    17161 aagccgctgg ggtttaaaac acccccagcg gctcgttttt acactattgt ctcagaaatt
    17221 atctatggtc cgtcgattac tcgatatccc tttcaatcaa caatgagtca tcgccttccg
    17281 gagcagacat tgggctgtat tgccacggat tatggtagct ctccatcgcc tgatagatca
    17341 cctgggccag ctcattatta ctggacgggt catagcacag caaatattcg gtatcgggta
    17401 gcggcggtaa gccatctact ccactcagca cacgcaggtc tggactcatc atctcgaccg
    17461 gccttgccgt cacgccaaga ccggctttta ctgccgcacg aactgccgga agcgtcgagg
    17521 cgacataagc cagtcgccat ggaatatctg ctttattaag cgtcgccagc accatatcgc
    17581 gaaacgggct aggatcatcc agcaatacaa gagggatcgg ctcgcctttt tgcaatacgt
    17641 attccgctgc gcagtaccag tgtgttggcg aggtacgcag gttcaatgcc ttaaacgttg
    17701 aaggacgatg ggtggttacc atcaaatcca cttcctggga ttccagcatt tctgccatat
    17761 aggcattacg tttaacgcgg acatccagcg ctagtttcgg ataaaccgaa ctcacgcgat
    17821 ttaacaggaa aggtaagatc gtatcggcag attcatctga agctccgata gttaacacgc
    17881 cctgaagatt actgaacatt aatgatgagc aggcctcatc attaaaacgc aggattttcc
    17941 tggcgtaacc aagaagttga atgccatgtt cagttaacag tttgttgcga ccgtgacgag
    18001 cgaacagttc tttcccaacg agttgttcca gacgctgcat ttgctgactt acggcggact
    18061 gagtacgaca cacagcggca gctgcggcag caaaagtgtt cagatcggca acagcaacaa
    18121 atgttctcag cagatcgagg tcgaggttaa ttatcggacg atttgcactt atcatatatt
    18181 atcacttact ggcggctcat actgagctgg ttaatgctgt gcacacacaa acaagcaatt
    18241 ccatttgtaa tgtgcctccc tggcagtttc atccggaacc cggacgaaag taaaaatgca
    18301 tatgagttgc actaaaaaag cgactcacat tgttccgtta taatgcctga agtggatcac
    18361 agaatatatc ttcaggaatc gcatatctat taagttactc actcttttgt atttatgaca
    18421 tgcgcgtgtt tgtataaatg taaatgtgag tccttgttcc actctcgtgc agcatcgctg
    18481 gtcatacgcg aacacgtaca aacagcaatg gtgaggcggc atcaagagca gggatccgtc
    18541 atttatgcga gcattttacc ccaaaaacct tttatttata agggtcattg cgaattatct
    18601 gatgcaaagt tatgttatgt taggcaaagt aatcttctat tattaataag cacaacaaaa
    18661 cctttttgaa tattaaataa taattaatta gcatcatcct catacattaa tcccgcttaa
    18721 caatagtttc acaccttgca cccattacga cacctcggaa atcatcacgc tgttaaactc
    18781 tccgtaatgc ggaaatatca tcatcacgat tcattagtga attcttaaac gaggcatttt
    18841 caaacaatta tcttacagat aaaaaaacca cactcacaat taagaatcag aacaattcac
    18901 tacataacat taattattaa gcatatacac ctcattattt tgccattatt aagttattaa
    18961 cagcacaatc aagccttccc ctctggcaaa atcttattct gcagaccttc aaaacgccgt
    19021 cctgggggag tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa
    19081 aaaggtcaag attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttacg
    19141 acatccgtgg tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac
    19201 tgaaactgaa catcggcaat cctgcaccat tcggttttga cgcgccagat gaaatcctcg
    19261 ttgacgtgat tcgcaacctg cctaccgctc aagggtattg cgattccaaa gggctttact
    19321 ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg catgcgtgat gttaccgtgg
    19381 aagatattta catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc
    19441 tgaacagcgg ggacgaaatg ttggttcctg cacctgatta cccactatgg accgcggcgg
    19501 tttcgctctc cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc
    19561 cggaccttga tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc gttattatca
    19621 acccaaataa cccaaccggc gcggtatatt ccaaagagct tttaatggag atagtggaga
    19681 ttgcacgtca gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctctacg
    19741 acgacgctga gcatcactca attgcgccgc tggcacctga tctgctgacc attaccttta
    19801 acggcctgtc gaaaacatac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg
    19861 ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc
    19921 tgtgtgctaa cgttcctgcg caacacgcca ttcagactgc actaggcggt tatcagagca
    19981 tcagtgaatt tattactccc ggcgggcgtc tttatgagca gcgtaaccgc gcgtgggaat
    20041 tgatcaacga tattccgggc gtttcctgcg tgaaaccgcg cggtgcgctg tacatgttcc
    20101 cgaaaatcga cgccaaacgc tttaatattc acgacgatca gaaaatggtg ctggatttcc
    20161 tgttgcagga aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc
    20221 acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg agtaagttcg
    20281 cgcgtttcct ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc
    20341 cggcagtgtt atcccgccac aatgacctga tgatgtcatc atacataagg tcactatgaa
    20401 acagagccat ttttttgccc atctctcccg cctgaaactc attaaccgct ggccgctgat
    20461 gcgcaacgtg cggactgaaa atgtgtccga acacagtttg caggtagcga tggtcgccca
    20521 tgcgctggca gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc
    20581 tttactggcg atgtaccacg atgcctcaga agtgctcacc ggcgatctcc ctactccggt
    20641 gaaatacttc aattcgcaaa tcgctcagga gtacaaggcg attgaaaaaa tcgctcagca
    20701 aaaactggtc gatatggttc cggaagagct gcgggatatc tttgcgccgt taattgacga
    20761 gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata
    20821 tctgaaatgt ctggaagaac tcgcggcggg aaataatgaa ttcttgctgg caaaaacgcg
    20881 actggaagcg acgcttgaag cgcgccgcag ccaggagatg gactacttca tggaagtctt
    20941 tgttcccagc ttccatcttt cgctcgatga gattagccag gattcaccgc tgtaagcagc
    21001 cggagtctgc gtcgcatcag gcaataagcg ccggatgcga catcaggctc ttgtcaaaac
    21061 ggaaacagca tcgggatcat caccacacaa accgccatca cgataatggt gaacggtacc
    21121 cccaacttca caaagtcact gaagctgtaa tttcccggac ctaaaaccag tgtgttaacc
    21181 ggtgaagaaa ccggcgtcat aaaggcggcg gatgctgcca tcgccacgac catcgcaaat
    21241 ggataaggcg acacacccat cgttttggca gcagccagcg caatcggagc catcaacacc
    21301 gccgttgcgg tattagagat aaatagccca ataaccgccg acaagacaaa caaacaaccc
    21361 agcatcatat gtggcccgta accgccgcca atgtccatca gccctttcac cgccagcgcg
    21421 acacctcccg ttttctgtaa tgccacagca aacggcatca tcccaacgat caaaataatg
    21481 ctcggccagt gaatggattt ataggcgctt tcagcatcga tacagcggaa tttccccatc
    21541 agcaggcagg cgatgatagc ggcaacagga ttaggaattt catcggtcag cattaacgcc
    21601 accatcaaca ccaggcaaaa aatggcatga ggtgcctgac tgtgtgctgg cgatgcttca
    21661 ctcacttctt ctggtaagtt cagcgccaca aagtcacgcc cttgttttgc cagcataccg
    21721 atcagtttcc agttacccac aaccaggatg atatcgccca gcagcagagg ctcatccgcc
    21781 agcgaacctt ccatcgcctc gccattgcgc tttagcccca ccacattcag tccgtagcgg
    21841 gtacgaaaac caatttcgcg caccgattta ccaataagtt ctgactcagg aattaatgaa
    21901 atctctgcca tgcccacatc aagtgcctgg tcagaaaaat actcgccgcg cagtaccatc
    21961 ggctccagca attgctcact acaaaattgc cggagatcga catcagccgc agacatatca
    22021 ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat taacgttcac gataacgcga
    22081 cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc cataacgctc acgtaatttg
    22141 agatcgtcca gccgttgacc aatcattggc gatccggggc gaatagccag acgtcgcgca
    22201 cgcccggtca gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg
    22261 cgctgcgggg tctgggtatc ccctttcagc atgaaacgca tcactaacat atacaaaata
    22321 cccagcacca gcacgaccag gccaataggt gttacgctaa agaaactaaa accatgatag
    22381 ccttcacgca gcaattcact gttgactacc aggttcggcg gcgtcgccac cagcgtcatc
    22441 atgccgctta tcagcccggc aaaacttaac ggcatcatca ggcgcgacgg cgacgtttgc
    22501 atacgcatgg caacgcttaa caccacggga ataaagatag cgacaacgcc ggttgaactc
    22561 ataaacgcgc caagccccgc gacggtcagc atcaacaaaa ccaacatttt gatttcactg
    22621 ttgcccgcca ctttgaccag ccatgtcccc attacggtgg caacaccggt acggaccaaa
    22681 ccatcgccaa taatgaacaa ggcggcaatc aggacaacgt taggatcgga aaagccggaa
    22741 aatacttcgg ggaccgtcag cgttccgctt aatgcaaacg cgacaataac aaacaaagcg
    22801 accgcatcca tacgcactct gcccgtcgca aacaagacga tggcaacggc cagtaatgaa
    22861 agaacccaaa tcaattcacc gttcacaact tatccttgtt aattgagggg gatgacttga
    22921 ttctgccata aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg tttcactgaa
    22981 taataacatc gccatttggc tgtttggtca cagtaatttg ctccagactg tggaggacca
    23041 aatcgacctc attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg acatgacaac
    23101 ccgccgccag gccagaaagc acgccagcgg gagcatcttc caccaccaca cactcctgcg
    23161 gcgcaagccc cagcagctgc gcgcctaaca gatacgcatc aggttctggt tttccgcgct
    23221 tcactcgctc agcggttaca aacacctctg gtgcgggaag cccagctact ttatggcgcg
    23281 ctcgcgctac cggcatggag ccagaagtca caatggccca cggaatacct gctttattca
    23341 aatgattgag taaggcgatt gcccccggaa gcgcggtaat accctcggtt tccgtggcct
    23401 cgatttgctc cagacacgta aactcggcgg caatatcagc ctcggatttg cccgccataa
    23461 aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc tcttccggcg
    23521 ctaacccatg acgtctggcc cagttgctcc acgcccgctc taccgcaggc agggaatcca
    23581 ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcggacc tcctcaggcg
    23641 ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca ggcatgccac
    23701 acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa accgtgagtt
    23761 ccagcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac ataaccgagg
    23821 taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc atcgatggat
    23881 tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc cataatgtcg
    23941 atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccag ttcacgcatc
    24001 tgtaatccat aaccacgctc aatgattgtt tgcaggcgac ggtaacgttc agcatttgcc
    24061 ggatcgagca tagtcatcat cttgtactgg ttagacaaaa taagacgttg cgcgttggtc
    24121 atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag gctgagtatg
    24181 ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag
    24241 ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac
    24301 gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa accagttgag
    24361 gatagcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc cctgcatggg
    24421 taaacttaag gcgaacagcg cagtggcaac tgccggccca agctgaccgc ccagggcaat
    24481 ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat
    24541 cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt
    24601 tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt
    24661 tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact
    24721 cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca
    24781 gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat
    24841 tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa
    24901 atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc
    24961 tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt
    25021 actggttctg aactgcggta gttcttcact gaaatttgca atcatcgatg cagtaaatgg
    25081 tgaagagtac ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg
    25141 gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc
    25201 gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac
    25261 tgctatcggt caccgtatcg tacacggcgg tgaaaagtat accagctccg tagtgatcga
    25321 tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc
    25381 tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt
    25441 tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc tctacgccct
    25501 gccgtacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca
    25561 cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat
    25621 catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt
    25681 tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc gttctggtga
    25741 tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat
    25801 caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg
    25861 ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta
    25921 ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg gtcgtctgga
    25981 cgctgttgta ttcaccggtg gtatcggtga aaatgccgcg atggttcgtg agctgtctct
    26041 gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt
    26101 cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta tcccaaccaa
    26161 cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag
    26221 ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc
    26281 gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc
    26341 cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc
    26401 gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgctaac
    26461 tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc
    26521 agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa
    26581 gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag
    26641 tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag
    26701 ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc
    26761 ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat
    26821 gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa
    26881 gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgct
    26941 gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat
    27001 gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc
    27061 gcacgcagca ttccacacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc
    27121 gcagaccgtc ctgacgtgct ggttgccgcg tgcctggctg ccatgaacgg cgtagaaatc
    27181 ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa
    27241 cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacttct
    27301 ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgagcg tatcgagaaa
    27361 gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgattctct gactgccact
    27421 tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt atcagctgac tgaacttgcg
    27481 cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg aaccgcgtac cgttaaggca
    27541 gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag
    27601 atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg caggcattga aatcgttgat
    27661 ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc
    27721 atgaccgaaa ccgttgcccg cgaacagctg gaagataacg tagttctcgg tacgctgatg
    27781 ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc
    27841 atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg
    27901 ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat
    27961 ccgactgcag aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc
    28021 ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg ggacttctgg tgctggtagc
    28081 gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg
    28141 atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg
    28201 ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt
    28261 aacaccacct acaaagcagt acagcgttct gccgatctga tctccatcgg gccgatgctg
    28321 cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc
    28381 tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaatctcg tcatcatccg
    28441 cagctttgcg ctgcggatat ctgaaccgga aatagtcact atttccggtt ttttattctc
    28501 ttaatttgca ttaatccttt ctgattatct tgcttagctg cgctgcatca atgaattgcg
    28561 ccatctcact ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc
    28621 gcaatcactg aatctaaacc aaccagaaga tgggcaatgc ccgatacgtt ggtgattatc
    28681 ttttttgttg ccattttaac cagtcttgcc acctgggtag ttccggtggg catgtttgac
    28741 agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgaaaagt cgtagatcca
    28801 cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg cgtacagctg
    28861 tttacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg gttaacctca
    28921 ggatcgaaat acgggacagc cgttggcatc atcatgttta tgctggtgat tggcggcgcg
    28981 tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat
    29041 acccgcggaa atgaaattct ctttattcct gcgctcttta ttctgttttc acttggcggc
    29101 gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc accgctaatg
    29161 gtccggctag gctatgacag tattaccacc gtcctggtga cctatatcgc cacgcaaatc
    29221 ggttttgcca gttcgtggat gaacccgttt tgtgtggttg ttgctcaggg gattgccggc
    29281 gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc cactctgatt
    29341 ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc
    29401 gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt
    29461 acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg ggtgatttgg
    29521 ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt caccatgggt
    29581 ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt taataccatg
    29641 gcttcatcct tcaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtaggtttc
    29701 gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag cgtgttaaat
    29761 accatcctca acagcattgc caatgccatt agcggtctgg ataacgcggt cgcggcctgg
    29821 tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc tggtcaggcg
    29881 gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt
    29941 accgtgctgg cgttccagtt tggtgatggc ttcagccaca tcatttaccc aacctcagct
    30001 tcgttaatgg cgacgctcgg tgtgtgccgg gtggacttcc gtaactggct gaaggtgggc
    30061 gcgaccctgc ttggactgct gtttattatg tccagcgtcg tagtgatcgg cgctcagttg
    30121 atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca ttctgccgtt
    30181 tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag cgctttcagc
    30241 gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggtgtcag ccagcaaact
    30301 tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcaact gaacaacgcg
    30361 ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca
    30421 ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg
    30481 gcctggacta caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg
    30541 gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta agttgcacga
    30601 tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac ttcgttctct
    30661 tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat tatcaggaaa
    30721 ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta cggattaatc
    30781 gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac gtcattatcc
    30841 agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc ggggttgaag
    30901 tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta caccagcaca
    30961 tcgttctggt ttaaagcagg cagattttcc ggcccaaaca gatgaccatg cgtcaaaaac
    31021 agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa atgcaaaagc
    31081 atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg tgccacttca
    31141 ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt acgcgggccg
    31201 tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc aaacaactcc
    31261 agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat cagtttcatc
    31321 actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc
    31381 agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc actggaatgt
    31441 cgcataatcc accaggcgtt ccggcacctc atcgccatgg agtaccaggc ggttaatcat
    31501 tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtgc
    31561 taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct ccgccgttaa
    31621 tggcgctttt ttcgcacccg caaagacaac atccgttgga cgttcctcgc ggatgggcat
    31681 cagatcgcta cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag
    31741 cggataaatg cgttcccagg tcggtggcgc aaatcgatct tccagatact ccgcaatggc
    31801 agaagattca ctcaactcaa aatcatcaat ttgtaataac ggcacacggc gtgtctgacc
    31861 gtaaccttgc cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg
    31921 aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag gggagaaaaa
    31981 atgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg caataaaggg
    32041 ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata ctcacgcaag
    32101 tcgccgcgct ttctatactt gtggtgttca gatgattaga caaccggagc tgcaatgatc
    32161 gatctctatt tcgccccgac accaaatggt cacaaaatta cgctgtttct cgaagaagca
    32221 gggctggatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt tcgcccggaa
    32281 tttttgcaca tttcgcctaa caacaaaatt ccggcaattg ttgatcattc tccagccgat
    32341 ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct ggctgagaaa
    32401 acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca gtggttattc
    32461 tggcaggtag gcggactggg gccgatgctt gggcagaatc atcattttaa tcacgctgcc
    32521 ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg tctgtaccat
    32581 gtactgaaca agcggctgga aaactcgccc tggctgggag gtgagaacta cagcattgcg
    32641 gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga ccttgcaatg
    32701 tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac cggtcaggca
    32761 ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg
    32821 tattatttca ttctaagtaa aacaacggag aacctgcaat ggcacaacct gccgctatta
    32881 ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa gaaattaaca
    32941 accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa gcgcgcacta
    33001 gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt attcagcatg
    33061 tagagaataa ccgtttctct ttgctggaaa aattaactca ggatgtgctc gatatcgcac
    33121 gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac gcgctgcgct
    33181 acgctgattc ggtatccatg accttaagct ggcagcgtta accgccatat cgggaggctg
    33241 catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga ttccacgttt
    33301 gctggagctg ggccatcaaa tcacggtaat gacgcgtaac ccgcagaaag ccagttccgt
    33361 tctcggccct cgggtgacac tatggcaagg gcttgccgat caaagcaacc tcaacggcgt
    33421 tgatgcggta atcaatctgg ccggagaacc gattgctgat aaacgctgga ctcacgagca
    33481 aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg atttgattaa
    33541 tgccagcgac acgccaccgt cggtactcat ttccggctcg gcaacgggct attatggcga
    33601 cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta cccataaact
    33661 ctgcgcccgc tgggaagaaa ttgcctgccg ggcacaaagt gacaaaacgc gagtgtgcct
    33721 gctgcgtacc ggcgtagtgt tggcaccgga tggcggtatt ctcggtaaaa tgctgccgcc
    33781 gtttcgtctt ggtctgggcg ggccgattgg ctcctgtcgg cagtatctgg cctggattca
    33841 tattgatgat atggtcaacg gcattctctg gctgctggat aacgagctgc gtggaccatt
    33901 taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc tcggtcatgc
    33961 gctgcatcgc ccggccattt tgcgcgtccc cgcgaccgcc attcgactgt taatgggcga
    34021 atcttcagta ctggtattag gtggacaacg cgcgctgcct aaacggctgg aagaagcggg
    34081 ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgttgttc gctgatgtgg
    34141 tttactgcaa acatccgcca gttaactcct ggtgttacag aattagtggc tttgcgcgat
    34201 aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta aacctctctc
    34261 cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg cgccttcttc
    34321 acgtaatctg tcagcaatag tcatcagttt ctccttttct tgtggtgcgc gttccgctat
    34381 ctcaccaata aatgcacgga aacgctgggc atctcccgtt tgtaatacat aattaaacag
    34441 ggcttttagc tgcctgtcat tagtgtttcc tgtaactagc agcgaaacaa tttggtcgac
    34501 taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca gcgccatttt
    34561 gcgatgttgc ataatctcgt catccggcac cacggtaata tccaccagcg gaaaagccga
    34621 tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc agagtgaata
    34681 aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg gaagctcttt
    34741 atagcctgca tcaagatggt tttgcatcgc cgcaatggaa tagcgcatca tgcgaaaagc
    34801 cattaattct tccggcttac tttggtgctc tatcactaca taaatataac ccgctccctc
    34861 ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt catcaataaa
    34921 actgtttggt tccagtttaa gcgttgttaa atcacacagt ttgcgcagcg gcgcgggaag
    34981 atgaatatca ataaaatccc gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc
    35041 atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccct caaatttaag
    35101 ataacgcgac aataaccacg attaacaccc aatgcagcag gctgttttta tctttggaca
    35161 gcgaattcag aactattttt agcggactgc aacttttaca tttcagcgga agctgactcg
    35221 caaattcccc ttgaaaatac tccggcagcg ttagccaccg gagggggaat tatttcagcg
    35281 atcccttaag gaactgttgc agacgagggc tttgcgggtt accaaataac tgttctggcg
    35341 caccctcttc ttctattttc ccctgatgga ggaaaatgac gtgagtagaa acatggcgag
    35401 caaagcccat ttcgtgagtc actaccacca tggttttccc ctcttctgcc agttgctgca
    35461 taatacgtaa cacctcgcct accagttcag gatcgagcgc cgaggtaggt tcatcaaaca
    35521 gtaaaacttc cggttccatt gccagcgccc ttgcgatgga aacacgctgt tgctgaccac
    35581 cggacagatg caccgggtat ttcccctgcg cacgttcgtc tatcccaact tttgccagat
    35641 acttcaccgc ccgctcgcgc gcttcctgct tgctcaggcc caacacctga atcggcgctt
    35701 ccatgacgtt ttccagcacc gtcatatggc tccagagatt gaagtgctgg aataccatcg
    35761 tcaggcgtgt gcgcagtaag cgcagctgat ttttatcggc gactttgagt tgaccgtctt
    35821 tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgatccttca ctcggttttt
    35881 cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat ccgatgatgc
    35941 ttatgacatc tccggcattc gcttgcagtg agaccccttt cagcacttcg tgttcgccgt
    36001 agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc tcagtgcgtt
    36061 gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag gctgatcagg
    36121 acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa aggttgatac
    36181 gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtagc ggtgaaagcc
    36241 agcgcagtag agtgcagcat caggatcact tcgttgctgt aggccggtaa cgcaatacgc
    36301 agcgccgatg gcaaaataat gcagcgatac attttaaagg tcgagaagcc ataggctctg
    36361 gcggcttcga tttccccatg cggcactgaa cggatcgctc cggcaaaaat ctcggtagtg
    36421 taagcgcagg tgttgagcgt cagcgccagc acggtacagt tcaggccact gcgaaaaaag
    36481 gcgttaagga actcggttcc cttaacaatt tccagcgtgt acatgccgga atagaacacc
    36541 agcaactgaa catacagcgg cgtaccgcga aaaatatacg taaataacca gattggaaac
    36601 tggatgtatt tattacttga gacgcgacca atcgccagaa acagcgccat gactccgcct
    36661 atcactaccg acaaaatcag cagccacagg gtgatcgcca cgccagtaaa gcgataaccg
    36721 tcagtccaca gcagcggttt ccagtattca tgtaagattt cgatcacagg tcagccctct
    36781 tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa acagtggtga
    36841 aaaccaggta aatcacgcca cagacgatgg cgaaatagaa cggttcccag gtacttttgc
    36901 ctgccagctg cgtggctttg accacatctt ccaggccgag taacgaaacg agtgcggtag
    36961 atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg
    37021 gaaacatgat ccgccgaaac acttgcccac gagtgaaacc gaacgccgtc gccgcctcta
    37081 tatgtccttt cggcactgcc ataaaagcgc cacggaacgt ttcggtaaaa taagcaccgt
    37141 agataaaacc gagagtaata ataccagcga ccatcggatc gatatcaatc tgcccgacgc
    37201 ccatcgcctc cgtcaccgtg tttagtgcaa tctgcaaacc gtagaaaatt agcagcatca
    37261 acaccaagtc cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa
    37321 gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca gcgagcacta
    37381 cagagctgat agccagctcc agcgtgacga gcgcaccctg taaaataaca cctgaaaacc
    37441 catacaacat gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg
    37501 gttacccgaa gggagcggac tttctttcac cgatggcgat taaccaccat aaacatcaaa
    37561 atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt cggcaaaggc
    37621 tttgttcagt gcctcgcgca gttcgttatc ttctttacgc aggcccatgc cggtccctac
    37681 gccaaacagt ttttcatctt taacagacgg gccaccgaat ttgtaatctt taccgacggg
    37741 ttgtttgagg aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat caatacgtcc
    37801 ggcagtcagg tcagaataaa tattgtcctg cccttgataa gaaacgattt caatgccttt
    37861 tggtgcccag tgttcgttac cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg
    37921 tttgcctttc agcgactcga ctgtcggctg aatgtcggaa tttttcgcca ccaccagacg
    37981 agaatcggca gcgtacagtt tgtcggtgaa ggctatttct tgctgacgtt tttccgtaat
    38041 ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga ttaacgcatc
    38101 cagtgggttt tcgacaaacg tacattgcgt attgatgcgt ttgcacaact ccttcgccag
    38161 atcgatatcg aagccaacca gttcgccttg tgaatttttt gattcaaatg gcgcataggt
    38221 cgggtcggta ccgatgcgga tgttttgcgg aatcgcagca aacgccacag ttgcgctgga
    38281 gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct caactgacag
    38341 tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa attgaccatc
    38401 aaggcaaaat atcccgcccc gttagacgta ttatttttca ttaaaaatgc ttaagtatgc
    38461 atttcaatgc accacgatag tgcaacaaac ctggcgtgca ccatgatgat cattccatca
    38521 ggtacagctt ccctgcgacg tatcagtcac cgtagacatt aaagtcaaaa tactttttcg
    38581 ccatcttgtc gtaggtgccg tcctgacgca gctcaccaag cgccttattg aaggcagccg
    38641 tcagttcagc atcatcttta cgtagcccta caccggtgcc gtcaccgaag tattttttgt
    38701 ctttcactga tgagccagca aaggcgaagt ctttaccagc aggttgcttg aggaatcctt
    38761 cgctggcagc aacttcatct tgtaacgcag catccagacg tcctgcagcc agatcggaat
    38821 agaccaaatc ctggttggca taggccacca catccacacc tttgctacgc caggtctcgt
    38881 tagcgtaagc ttcctgggtt gatccctgca gcacaccaac atgtttacct ttcagtgaat
    38941 ccagcgttgg ctgaatcggc gaacctttgg cggcaatcaa acgagaatct gcggcgtaca
    39001 gcttgtcgga gaaagcaatc tcctgctgac gtttatcggt aatggaaagc gacgaaataa
    39061 tggcgtcgat ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc
    39121 aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata tcaaagccaa
    39181 caaaatcacc tttagcatct ttcgatgaga acggcgcata ggtggtatca gttccgatac
    39241 gtaccgtctc cggtagcgcc gcatagctgg aagccgctgc ggagagaccg actaacaaag
    39301 acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct tttttatgtt
    39361 gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat cttgacgaca
    39421 acagtaacat tcaacgttaa atatgttaat aagacgttgc attattgtcc tgaagttgaa
    39481 gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa caaaatgtcg
    39541 aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag ttatgcgccc
    39601 tgccagcggg caaagagatc ttcaggaagc gttatggcga attgatcgag tacacggtta
    39661 accgtctgat ttatcacatc atcaagggat tgcgggcgat gataaaacgc cggaacggga
    39721 ggcataatca ccgcaccgat ttctgctgcc tgagtcatta aacgcagatg gcctaagtgc
    39781 aatggtgttt cacgcacgca gagcaccagc gggcgacgct ctttcagcac aacatctgcc
    39841 gcacgggtca gtaagccatc agtatagcta tggacaatgc cggacagggt tttgatcgaa
    39901 cagggtaaaa tcaccatccc cagcgtctgg aaagaaccag aagagatgct ggcagcaata
    39961 tcgcgcgcat cgtgcgtcac atcagctaac gcctgcactt cgcgcaggga aaaatccgtt
    40021 tcaagagata aggtctggcg cgcggcctgg ctcattacca gatgcgtttc aacatctgcg
    40081 acatcgcgca gaacctgtaa taagcgcacg ccataaatcg cgccgctggc accgctgatg
    40141 cccacaataa gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag actttgcagg
    40201 atttcggcgg gagttgcaag tcagggtgcc agaccggcac cctgagcgaa ggcatcatcc
    40261 ttcgttatgc atttcgagat tttccacttc gttctgacgt tgcacggctt tggcgtcatc
    40321 attacgtaaa gtatcaagga aatcgaggta gccctgatca acatctttgg tgacgtagac
    40381 gccgttgaac accgagcatt caaactgctg gatatccggg ttttcagcgc gaacggcttc
    40441 gatcagatcg ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc
    40501 atctacttcg cgaccgtgag cgatcagttc cgtggcgctc ggcatatcaa taccataaac
    40561 gttcgggaag cgaatttccg gtgccgcaga agcgagatac actttcttcg ctccggcttc
    40621 gcgtgccatc tcgataatct gctcagaagt ggtgccacgg acgatggagt cgtcgaccag
    40681 caggacgttt ttatcgcgga actcggcgcg gttggcgttc agtttacggc gcacggactt
    40741 acgacgcagc tgctgacccg gcatgatgaa ggtacggcct acatagcggt ttttaacgaa
    40801 gccctgacgg tacggtttgc ccagaatacg cgcgatttcc agcgcgatat cacaagaggt
    40861 ttccgggatc gggatcacca cgtcgatatc cagatcttcc cattcgcggg caattttctc
    40921 gcccagtttc gtgcccatat tcacgcgggc gctgtaaacg gaaattttgt cgatgaacga
    40981 gtccgggcga gcaaagtata catactcaaa caggcacgga ttgctgaccg gattgtcagc
    41041 acattgacgg gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc caggcgcgac
    41101 gtcacgcagg aaatcaaagc ctagtgtatc gagcgcaacg ctttcggaag cgaccatata
    41161 ttctgtgcgg ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa tcccgtttgg
    41221 atcgcggaaa gcaaccatac catggccgat aatcatcgcc acgcaggcat acgcgccgcg
    41281 gattaagcgg tttgtggcag caatggcagc gaaaatattg tcggcttcca gcgggtagtg
    41341 gcggaagttg tccagctcgc tggcgaagat attaagcaga atttccgagt cggaagtggt
    41401 gttgatgtgg cggcgttttt cttcaaacag ttttttacgt aactcgtgag cattggtcag
    41461 attgccgttg tgggcaagcg taatgccata cggagagtta acgtaaaacg gctgcgcttc
    41521 agaggcgctg gagctgccag ccgtagggta acgcacatga ccaatgccca tattgccctg
    41581 caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg ctttacgcaa
    41641 acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac cgcgatgctg
    41701 aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg cgataccgac
    41761 aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt acgctctggg
    41821 caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa aactgaactg
    41881 cgggatcagc tgtgatttgc tccagtcttc gcttttcgac accccggtaa aggagtcgag
    41941 aaagaagaga atggcggcaa caatcaacac accgcgcaac gcaccgaaac agacgcccag
    42001 cacccgatcg gtgcctgaca accccgtttt ctccaccaac tggcctatca cgaagttcac
    42061 gatagcacca acaatcaggg tagcgataaa cagtaccgcg atggcaatcc catttcgaac
    42121 cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat gactggcaac
    42181 aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc cgcggatcag
    42241 gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc agaccatatg
    42301 tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa
    42361 cgtttgcgta gggatttcct tcccacgcat caataaaaat ggcgctgaaa aaatattcaa
    42421 cgccatcgac tttttatgcc tttgcggcat cgggcaagac cgtattaatt cggcgtatag
    42481 cccattacca cgccacttaa gccagaaagc tgcttgagct cacccagcga tcctttcagc
    42541 ttctctttgg aagcatcagg cccaaccaga atacgggtaa ttttaccctg cactggcgtg
    42601 gatggcgacg tataaacccg ataaccggca ccgcgcagct taccgacaat ctcattcact
    42661 ttatcggcat ttttcagcgc gcccagttgc acaacataag ctttacccgt tggcgctgct
    42721 ttttcttcca cgaccggctt cggttctggc ttcggcgcag gtggtgcttc aaccttcggt
    42781 ttaggcgact ccaccggttt cggcttcggt ggggcgaccg gtgcaggttc cggttcaaac
    42841 tcggtgttat tggctgcaat agtggccgga tcgagcgacg gtgctgcggc atcacctgcc
    42901 cgcacctctt ccgctgcgcc ttccggcggc tgcgtcggca gcgcctgggt ggcggctggc
    42961 atcatatcag gctcatcgcg atcgcccgct tttggcacca gcgggatagc cgcgaactca
    43021 tcctgataat gttttttctg cccgtccagc agccctggaa gtacaatcac ccccagcgcc
    43081 accagcacga tcgtgcccac taaccgattc tgaaacttac ttgccaccgc ttctcctcgc
    43141 gtcaatcact tccatgacat gtgcgaccgt gtggaacgaa ccacacacca gcacggtatc
    43201 ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc gccacgctat caaatgattt
    43261 gccgttaccc aaatgctcaa gcagttgttc tgccgtggca ccgcgcggcc cttccagtgg
    43321 cgcacaatac cagtcatcaa ccacgctttt caaccaggcc agagttccgg caatatcttt
    43381 atcatgtagc ataccgataa ccgccagcac gcgcccgttt ttcggtagcg ctttcatacg
    43441 cccggtgaga tattccgccg catgtggatt atgcgcgaca tcaaaaataa cacgtggcga
    43501 ctcgctcaca atctggaaac gtcccggcaa aattgcgctg gcaatcccgt cgcgaatggc
    43561 attttcactg acctccagcc cgctggcacg cagtgccgcc agcgccgttg cggcattcgg
    43621 ttgcgggaca agcggcaacg gcagattttc cagcgtgccg tgagcatcgc taaacgtcca
    43681 gtcatgatcg gtgacggaat agttccactc aacgccccga cgttgtaaca gtgcactttt
    43741 ttcctgcgcc acatcagcaa tggtagaagg catttccggc tcaccgacaa ttgccggttt
    43801 ttcgctgcgg aagatgcctg ctttctcgcg accaatgctt tcgcgatctg gccccagcca
    43861 gtcggtatga tccagcgcaa tgctggttac aaccgcgaca tcggcatcga caatattggt
    43921 tgcgtccaga cgaccaccca gccctacttc cagaatcacc acgtcaagtt gtgcctgctt
    43981 gaacagccac aacgccgaca gcgtaccgta ctcgaaatag gtcagggaaa tatcaccgcg
    44041 tgccgattca atctccgcaa aagaggcggt gtgggccgat tccggcaatt cctgaccctg
    44101 cacacgtacg cgctcggtat aacgcaccag atgcggtgaa ctgtagacgc ccactttgta
    44161 ccctgccgcc atcagaatcg actccagcgt acggcaggtg gtgcctttgc cattcgtacc
    44221 cgcaacggta aacacaaatg gcgctggttt cagaacgcca agacgcgccg cgacctggct
    44281 cacgcgctca aggccgagat cgatagtttt actgtgcagg ttttccagat aagaaagcca
    44341 cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata atcatggtat ccgctgattc
    44401 gttacggtga gaatagcaaa agggcagagc cagtggccct gcccttatca gttatcaggc
    44461 ctcaggttcc tgatctggta ccggtggcac cactacgcct tcacgcggcg cttcaggatt
    44521 cggcgctggc agattcatca acttcgccag aatactcgcc agtttcaggc gcatttccgg
    44581 acgacggacg atcatgtcga tcgcgccctt ctcgatcagg aattcactgc gctggaatcc
    44641 tggcggcagt ttttcgcgaa cggtctgttc gataacacgc ggaccggcaa agccgattaa
    44701 cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg aaacttgcag aaacaccacc
    44761 catggtcggg tcggtcagca cggagatgta cggcaagccg cgctcctgca tttttgccag
    44821 cgccgcagag gttttcgcca tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc
    44881 accagaggcg gagaagcaga tcagtgggca gttatcttcc agcgcctgct caacggcacg
    44941 cacgaaacgt gcacctacca cagaccccat ggaaccgccc ataaaggcga actcgaatgc
    45001 cgcagcgaca actggcattc catacagggt gcctttcatc accaccagcg catctttttc
    45061 gccggtttct ttctgcgcag atgccagacg gtctttatac ttcttggagt cacgaaactt
    45121 cagcacatct ttcggctcaa gctcgctacc cagctccaca aggcttcctt catctaacag
    45181 gctatgcagg cgattacgcg ctgtcatacg catgtgatgg tcacacttcg gacagacctc
    45241 aagattacgt tccagctcag cgcggtataa aacctgaccg cagctatcac acttagtcca
    45301 caccccttca ggaatgctcg ccttgcgggt gggagtaatg ttgcttttaa ttcgttcaat
    45361 ccagctcatt agggaccttt ctgtctgaac ctggttcgat gccagtttta tctttgggga
    45421 cgcataatgc catttttgcc cccaacagac catgaatgtt gcacattaaa acataacagc
    45481 ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta ctttttattt tgcggcgcgt
    45541 gccgcagcgc gtttgtggcg aataaattcg aaaacacctg gcaatattga aacaaaaata
    45601 atgccaacaa tcatcagttt aaggttgctc tgaatgaagg ggagcgttcc gaagaaataa
    45661 cccgcgtagg taaaaatcag tacccatagc aacgcgccga tgacgttaaa agcggcgaaa
    45721 tgacgatacg acatgtggcc cattcccgca acgaacggcg cgaacgttct gacgatcggc
    45781 acaaaacggg cgagaataat cgttttgccg ccatgtttct cgtaaaactg atgagtctta
    45841 tcaagataac tgcgacgaaa gattttcgaa tccggattac tgaacagttt gtcaccaaat
    45901 aaccgtccaa tggtgtagtt caccgcgtca cccacaatcg cggcgatcag catcagcacc
    45961 accatcatat ggacgttgag atcgttggtt tccagtgatg ccaacgcacc ggcaacaaac
    46021 agcaatgaat cacccggtaa aaacggcgtt accaccagac cggtttcaca gaacaaaatt
    46081 aagaacaaaa tggcataaac ccagacgcca tactccgcga ccagttctgc cagatgcacg
    46141 tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt ccatattagc ctatgccttg
    46201 tactcgtcat cttacaggct gtaactacgt tggctacagc ctgaaatgtt ccgagcatta
    46261 ttcttgttag tccgccagaa atagcgggcc cattggcggt tttggaagat cataccggtc
    46321 agggtaatcc accgcgacca gatacagccc ttccgctttt gccgttgctg ccgccagcgt
    46381 tctgtccttt gccgccagca actctgctat ccagctctcc ggctggttgt gggcaccgac
    46441 ttccatcagg ctgccgacaa tattcctgac catatgatgt acaaaggcat tcgctttgat
    46501 atctaccacc acataaggac cgtgacgcgt gacattaata tgcataacgt tgcgccacgg
    46561 ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa tcattctcgc ccagcaagca
    46621 ttgcgcggcc cgatgcatcc gttcagcgtc cagcggttcg taaaaatggg ttaccccttt
    46681 actcagtacc gccgggcgca gccgatgatt gtagatgatg tagcgataac ggcgagccgt
    46741 ggcgctaaat cgggcatgaa aatcatcagg tacagcttta acccaacgca cggcgatgtc
    46801 accaggtaaa ttcgcattta cgcccaacgt ccacgcagcg tctttgcgct gcgcggtcgt
    46861 ttcgaaatgc acaacctgcc cggtgccgtg tacacctgcg tcagtacgcc cggcgcagaa
    46921 gacggtgatg ggttcgttcg ccacctggga gagcgccttt tccagcttct cctgcacact
    46981 gcggacttcg ttctgccgtt gccagccgta atacttactg ccgtcgtact caatgcccag
    47041 cgcaatttta taaactggcg gttgttgctg gtcggacatt agtacagata ctcctgcacc
    47101 agtttctcgg cgatttttac tgccatcagc gcgccgccaa agcgaacgtt atcggccacc
    47161 gaccagaact gaacttgctc cggcataccg tagtcattac gtacgcagcc aacggaaaga
    47221 tgcggcgtac cagaagcatc ccccacctga gtcgggaatt cgttctcttc agagagcaca
    47281 atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg ctgccagcgg acgcagtgct
    47341 tcaaagttga ccatctgggc atgaccgtag aataccggtg cctggacgac gcttgccgaa
    47401 atcatcagcc cttcgtcctg caggattttg cgtacttcgt caacgatacg acgttcttca
    47461 cgcacgctac cttcgctatc cggcagtaac ggcagcatgt tgaacgccag ctgacgcccg
    47521 aagaaatctt cttcatcaat cggaatgccg ttgagcaatt tagcactctg ccccgctaac
    47581 gcatcgaccg cttttttacc ctgggcggag gctgaaatca ggctggtaac gctgatacgc
    47641 gacaaaccgc cctgatcgat taacggtttc agtgccgcca gcagttggct ggtcagactg
    47701 tccggtacgg cgatgacatt ccggttgcgg taatcggtca gtacaaacgg gtttacttcc
    47761 ggcaccacca gcggtacgtc aggttcgaga gcaaacaatc cgctgctatc gatcaccagg
    47821 caacctgagt tggtcgcttc ttcaacccag gcagcggtag cttctttgcc tgcgacaaaa
    47881 aacgccagct gcgcctgcgt ccagtcgaat tcagcggcat cctgcacggt gattgtctta
    47941 ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg ccagtgcata aatttcccca
    48001 accgggaact gacgttcagc cagcgtttca agcagggctt cgcccacagc gccagttgcg
    48061 cccaggacgg caatgttcca gccttcagac atggtggttt actccagaaa tagcaaagct
    48121 ccctgccaat attacagcag agagcatgaa gaagagatta acgtgccgga tgatgaacgg
    48181 cgttaaaacc cagtttacac agcaatgatg ccgcgctggc gtcatcacaa atcacataca
    48241 gagatgacca ttcacggcgc tcaagatagt ttttgcgcag tttatcgaac tcacccggta
    48301 tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac atcatacacc aaatgcacca
    48361 gccttttcag cgttggctga tcgagcgggc catgcagcgt aatgcgacca aactctggcg
    48421 cgggcagtaa tgtatccagc gcaacgtgct gttcatgccc aataaacttg ctataagctt
    48481 caaacacttg cgtagtaccg cgtgctttac cttccagggt atagcctgcg atatgcggcg
    48541 tgccgatatc cacttttttc agcaattcca cgttaagttc cggttcgcct tcccagacat
    48601 ccagcactac gcttaacttc tggccttcgt tcaggcaggt caatagggca gtattatcga
    48661 cgactgcgcc acggcaggcg ttaatcagaa tcgctcctgg tttcaggcta cggatcagtt
    48721 tttcatccgc cagatgcagc gttttgtacg gaccatcttt aaagagtggc gtatggaaag
    48781 tcagaatatc cgcatgctga actaattcat ccagcgagcg gaaatccccc tcatccccac
    48841 ggtcggcgcg aggcggatcg caaagcaagg ttgtaatccc taacgcttcc agtcgcgcct
    48901 gtaaacggcg accaacgtta ccaacgccca cgatccccac ggtacgctcg tgcagtgaaa
    48961 atccatcacg ttcggcaagc atcagcagtg aggagaaaac atattccacc accgcaatcg
    49021 cattacagcc aggtgccgcg gaaaaaccaa ttcccgcctg ctttaaccac gcttcatcga
    49081 catggtcggt ccccgcggtg gcagtgccaa caaatttaat gggtcttcct gccagcaaag
    49141 attcattcac tttcgtgacc gaacgcacca tcagcgcatc cgcgtctgcc agttgagcga
    49201 cggggattgg acgcccggga accgcggtca cctcacccaa acggctaaat aagtcgcggg
    49261 cataaggcat attttcatca acaaggattt tcacgtttgt gttacctgta tgagacgaga
    49321 gttaaccgga caagtgtgcc ataatctcgc ggccaggcat acttgcgaag atttcaggta
    49381 taaggatacg taatgataca acctatttcc ggccctcctc ctgggcaacc accaggtcag
    49441 ggagataacc tgccgtctgg cgcgggcaat cagcctttat ccagtcagca acgtacttcg
    49501 ctggaaagct taatgacgaa agtgacctca ctgacgcaac agcaaagagc agaactgtgg
    49561 gcgggtatca ggcacgatat tggtctgtcg ggagattcac cgctgctttc gcgtcacttc
    49621 cctgccgctg agcacaatct ggcgcaacgt ctgctggccg cgcaaaaaag ccattctgcc
    49681 cgccagcttt tagcgcaatt aggggagtat ttacgtctgg ggaataatcg tcaggcggtc
    49741 acggattata tccgtcataa ctttggtcag acgccgctga atcagctctc accggagcaa
    49801 ttaaaaaaca ttctcaccct gttgcaggaa gggaagatgg ttattccgca accgcagcag
    49861 cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca atgcgcttaa acagctggtg
    49921 accaaacttg cggcggcaac gggagaaccc agcaagcaga tctggcaatc gatgctggaa
    49981 ctttccgggg tgaaagatgg tgagttaatt ccagcgaaac tgtttaacca tctggtgacc
    50041 tggctgcagg cgcgtcagac gctaagccag caaaatacgc cgacgctgga atcactacag
    50101 atggcgctga aacaaccttt agatgccagt gaactggcgg cgttatcggc atatatccag
    50161 caaaaatatg gtctttctgc gcaatcatcg ctttcttcag cccaggccga ggatattctt
    50221 aatcagcttt atcaacggcg ggttaaaggg attgatccgc gtgatatgca accgctgctt
    50281 aatccttttc caccgatgat ggacacgttg caaaatatgg caacgcgtcc cgcgctgtgg
    50341 atactgttag tcgcgattat cctgatgctg gtctggctgg ttcgttaacc ccgacgaaac
    50401 gacagtatcg tgacaataat tcccagcacc gcagagatcg ccccggcaag aaataccgaa
    50461 gagtaaccaa acgtggtcgc cagcattccc gccagcggcc cggagacgcc gagggcgata
    50521 tcctgaaacg cggcgtaacc gcccaatgcg gtgccgcgaa cttgtgaggg gacgcgttta
    50581 accacctcca cgcccagcgc aggaaagata agcgaacatc cggctccggt taacgccgcg
    50641 cccgctaatg cgacccacgc accaggggct tgccagagca gcaacaagcc caccgtttct
    50701 acaagcagag agacaatcgc cactttcacg ccgccaaaac ggtccggcat ccagccaaac
    50761 atgacacgca tcacgacaaa tgcgccgcca aacgcggtaa gagtaaagcc cgccatcgcc
    50821 catcctttgc tggcaaagta gagcgaaacg aaagtcccga taaccgcaaa accaacgcct
    50881 tgtagtgcca gacctaaccc aggtttccag ataagcccga caacactcca cagcgatgga
    50941 cgttctcccg ccagggccgg tactttgcgc actgtgccgt tacaggccca cgccagtaag
    51001 ggtaatgcca ttgtggtgag cgccagtgcg gcaaaaccgt aatggctatg aatcaatagg
    51061 ccaagcggcg caccaacagc gagggcaccg taaatcgcca ttccgttcca tgacatcact
    51121 ttgccggaat gttttggccc aacgatgcct aatccccagg tcagagcgcc cgtcagtaac
    51181 tggctttcac caaagccaag aatcaaacgc ccgacgacca gcagggcaaa tttgaacggt
    51241 gcggagacgg gcaaaatcgc cgccaacagc agcgcgccgc ccgccagacc acaagctaac
    51301 attccctgaa gcgccgaacg ttttgcgcca tattgatcgg ccagtcgccc ggcatagcca
    51361 cgcgtcagca ccgtagccag aaactgaatg ccgacggcaa tcccgaccat ggtgttgcca
    51421 tagcccagtt catgatgaac aaacagcggg ataaccggca acggcagccc tacggtcatg
    51481 taggtgagaa aaaccgcaaa agcgatgcgg aagagcgaaa aattggcaga agatcgtgtt
    51541 tctgtttggc ttacagcagt catgcattac tccagaatgc agcgcaaggc gaggagtctc
    51601 cccgtctcat ctctctggtt ttcagggtta cggtgcgttg gcaggattta acgcgtacgt
    51661 cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aacaggcggc gattgtcaat
    51721 aatgtgaaaa agggaaccat cgggttccct ttggcgttag tgccggaggc cgcattggcg
    51781 cgtaatgtcg gaggcgacgc tggcgcgtct tatccgacct actgcgaatt aatctttcag
    51841 cttgcgcatt accagcgtgg cgttggtgcc gccgaagccg aagctgttag acataacggt
    51901 ggtcagttcg cgatcggtcg tttcggtcac gatgttcaga cccgcagcct gctcgtccag
    51961 ctcttcaatg ttgatgctcg gggcgataaa gccgtgttcc agcatcagca gagagtagat
    52021 agcttcctgt acgccagcag cgcccagaga gtgaccggtc attgctttgg ttgcagaaat
    52081 cgccgggctc ttatcgccga acacttcacg gatagccgcc agctctttca cgtcgccaac
    52141 cggagtcgaa gtaccgtggg agttcaggta gtcgattggg gtatcaacac cgtgcatcgc
    52201 catcttcatg caacgcactg cgccttcgcc agacggagca accatgtctg cgccatcaga
    52261 cgttgcgccg tagccaacga tttcagcgta gatatgagca ccacgtgcca acgcgtgttc
    52321 cagttcttcg accaccacca taccgccgcc gccagcgata acgaaaccgt cacggtgagc
    52381 gtcataagta cgggaggctt tttccggggt gtcgttgtat ttggtagaca gcgcacccat
    52441 tgcgtcgaat tcgcaggcca tttcccagca cagctcttcg ccgccgccag caaacacgat
    52501 gtcctgtttg cccagttgga tctgctctac tgcgttaccg atacagtgtg cggaggtcgc
    52561 acacgcggag ctgatggagt agtttacacc gtgaatttta aacggcgtgg cgaggcaggc
    52621 agaaacgccg gatgccatcg ctttggtgac cacatacggg cctaccgctt tcaggccgcg
    52681 cgggccgcgc attgcgtcag cgccgaacac ctggaaacgc ggggagccgc cgccggaacc
    52741 tgcaatcagg ccaacgcgcg ggttattctg gtaagcttcc ggggagaggc cagcatctgc
    52801 aattgcctgc tccatagaaa ggaatgcata aatggatgcg tcgctcataa agcgcacaac
    52861 tttgcggtca atgaggccag tggtatccag ttttacgttg ccccagacgt ggctacgcat
    52921 gccggaatcc ttcagctcct gagagaaagt gatccctgaa cgtccttcac gcagagatgc
    52981 caggacttcc tgctggttat taccgatgct ggaaacaatg cccaggccag taatcactgc
    53041 acgtttcatt caatacctct gtaagtcgca catagagtaa gtttcgaatg cacaatagcg
    53101 tacacttgta cgccgaacaa gtccgatcag ccaattaata gagaaatttg cgcagcctga
    53161 cacacatcgc taagatcgag gcaccgcctg taagacgagt aacttacgtg aaacacaacg
    53221 ccatacaacc tgccaacctc gaatttaatg ctgagggtac acctgtttcc cgagattttg
    53281 acgatgtcta tttttccaac gataacggac tggaagagac acgctatgtt tttctcggag
    53341 gtaaccaatt agaggcacgc tttcctgagc atccacatcc tctgtttgtg gtagcagaga
    53401 gcggcttcgg caccggatta aacttcctga cgctatggca ggcatttgtt cagtttcgcg
    53461 aagcacatcc gcaagcgcaa ttacaacgct tacatttcat cagttttgag aaatttcccc
    53521 tcacccgtac tgatttagcc ttagcgcatc aacactggcc ggaactggct ccgtgggcgg
    53581 aacaacttca ggcgcagtgg ccaatgccct tgcccggttg ccatcgttta ttactcgatg
    53641 aaggccgcgt gacgctggat ttatggtttg gcgatattaa tgaactgacc agcaaactgg
    53701 acgattcgct aaatcaaaaa gtagatgcct ggtttctgga cggctttgcg ccagcgaaaa
    53761 acccggatat gtggacgcaa aatctgttta acgccatggc aaggttggcg cgtccgggcg
    53821 gcacgctggc gacatttacg tctgccggtt ttgtccgccg tggtttacag gaagccggct
    53881 tcacgatgca aaaacgtaag ggctttgggc gcaaacggga aatgctttgt ggggtgatgg
    53941 aacaaacatt acccctcccc tgctccacgc cgtggtttaa ccgcacgggc agcaacaaac
    54001 gggaagtggc gattatcggc ggcggtattg ccagcgcgtt gttgtcgctg gcgctattgc
    54061 gtcgcggctg gcaggtaacg ctttattgcg cggatgaagc ccccgcgctg ggtgcttccg
    54121 gcaatcgtca gggggcgctg tatccgttat taagcaaaca cgatgaggcg ctaaaccgct
    54181 ttttctctaa tgcgtttact tttgctcgtc ggctttacga tttattgccc gtcaaatttg
    54241 agcatgactg gtgcggcgtc acgcagttag gctgggatga gaaaagccag cataaaatcg
    54301 cacagatgct gtcgatggat ttacccacag aactggctgt tgcggtcggg gcgaatgagg
    54361 ttgaaggcgt tacaggtgta gtaacaaatt gtggcggtat tacatatccg caaggtggat
    54421 ggctgtgtcc agcagaactg acctgtaatg tcctggaact ggcgcaacag caaggtttac
    54481 agattcatta tcaatatcag ttgcaggatt tatcccgtaa ggatgactgt tggttgctga
    54541 attttgcagg agatcagcaa gcaacacaca atgtagtggt actggcgaac gggcatcaaa
    54601 tcagcggttt cagccaaacg tcgtctcttc cagtgtattc agttgctggt caggtcagcc
    54661 atattccgac aacgccggaa ctggcaaagc tgaaacaggt gctgtgctat gacggttatc
    54721 tcacgccaca aaatccggcg aatcagcacc attgtattgg tgccagttat catcgcggca
    54781 gcgaagatac ggcgtacagt gaggacgatc agcaacagaa tcgccagcgg ttgattgatt
    54841 gtttcccgca ggcacagtgg gcaaaagagg ttgatgtcag tgataaagag gcgcgctgcg
    54901 gtgtgcgttg tgccacccgc gatcatctgc caatggtagg taatgttccc gattatgagg
    54961 caacactcgt ggaatatgcg tcgctggcgg agcagaaaga taaggcggta agcgcgccgg
    55021 tttatgacga tctctttatg cttgctgctt tgggttcgcg tggtttgtgt tctgccccac
    55081 tgtgcgcaga gattctggcg gcacagatga gtgaagaacc gattccgatg gatgccagta
    55141 cgctggcggc gttaaatccg aataggttat gggtgcggaa attgttgaag ggtaaagcag
    55201 ttaaggctgg gtaaccagct ttggcattgt ttgtcggatg cggcgtgaac gccttatccg
    55261 acctacgtgt gacgtgtagg cctgataaga cgcgataagc gtcgcatcag gcatggtgct
    55321 ccagacgccg caacttactg ctgtgacgcc tgctgaaaca ggttttccca catatcggtc
    55381 accagcgcct ggtcacgcgg cgacaactca ccggcaccaa tggctttttc cagactctgg
    55441 ctaacggtcg tatgtaccgc ctgggcggag tggtcatcac cactttccag ttctgcgatg
    55501 gctaatgtca ggtggccacg caaataccca ctggcaaaca actcatcatc actggcatgg
    55561 tcaaccatac cgtcgattaa tgccagaatg cgtgattcaa actccgcgat catcttcttt
    55621 cctcattgta aaacgtggcg ctggctttag ttgagcgctt ccggccacgg gaactgttcc
    55681 gccgtaagtt ccggcgtgtg ataataattt tgtaatgctt taatgaagcg tgccgggcgt
    55741 tcaggaatgc cgttttccag ataatccatc acctgtgcat gtacccgccg ttgaaacacc
    55801 acacgatccg gttcgaagtc gccttccaga ttgtcgcaac tgacgttaaa cggatatccc
    55861 gccgccacgc agaacaacca gtcgagcgcc tgcggcttca cttcaacatc ttcaaactgg
    55921 ctttgcgttt gggcatcgcg accgtccggg caataccagt agccgaaatc aaccagctca
    55981 cggcgcgctt ttccggcaat acaccagtgc gaaatctcat gaattgcgct ggcataaaag
    56041 ccatgagcaa agacgattcg gttatacggt acttccgcat cagcaggaag atagatcggt
    56101 tcgtcgtcgc ctttaatcag acgggtatta aaatcatcgg caaagcagct attaaaaatt
    56161 tcaatcaact gctcatagtg gtgtgtactg ttcattagtt catccccaac cagtggagga
    56221 tctcctgtcc gtggctatcg taaagtaatt tggcactcat caccgctgag acaataacaa
    56281 tcatcgggcg gatcagcttt tgtcctttgc tcaacaccag tcgtgaaccc atgcgcgcgc
    56341 cgaggaactg ccccaccagc atcacgaatc ctgtcgccca aatcactttg ccaccgagaa
    56401 taaacagcag taaaccgccg atatttgacg ttgcgttgag taatttggcg tgagccgtgg
    56461 ctttggcgag gttgaatccg cacagcgtaa caaaggccag cgcgtaaaac gatcccgctg
    56521 ccggaccaaa gaagccgtca taaaaaccga cgcagccacc ggcaattaac gcgaagggta
    56581 aaccgtacat ccggcgctgg cgatcttctt cacccagctt tggcatcagc aagaaataga
    56641 ggccgataca aatcaccaga atgggcaaaa tctgccgtaa gacatcagcc tgaacatact
    56701 gcaccagcaa tgcgccgctc attgagccga caaaggtcat ggcgatattg agtttctgat
    56761 cgcttaaact aaccactttg cggcgaataa agtagatagt ggcggaaata gagccaccgc
    56821 aggcttgcag tttattggtt gccagcgcgt tagcgggaga catccccgcc gccatcaatg
    56881 ccggaatggt gagtaaccca ccgccaccgg caatcgagtc gataaatccc gccagcatgg
    56941 cgacaaaaaa gagaactccc agcaacagcg gggaaaccat aaacaggcta ttaaacgttt
    57001 ccattagatc acgtgctcat ccagtagcgc ctggcaggaa ggcggcaacg gaggcggtgt
    57061 cttcttctca ggctttgttg ttcccggttt tggaggttca aaccagcttt gcagttctgc
    57121 cccgcaacca tcgcctggtg gcggtaaagg ttgatcttca cacttcagac tatcggcagg
    57181 acaacgtaat cgtacatgca tatgcgcgcg atgctggaac cagggtcgca ctttgcgcaa
    57241 ccagtcgcga tcggtgcccg catcaaggca aagttgttgt ttaatcgccg gattaacaaa
    57301 aatgcgcgtg acgtctttgt cctgggcggc gagtttgatc aagctgaaaa tttctggctt
    57361 ccacagcgtc ggaacaacgt gtttaccgtc gcgggaaact aaatctaacg cttgcgggcg
    57421 caagagctgc gcggaggtcc agcgtgtttt tggcagttgc aggaagatgt ccacatccag
    57481 tccggtctgg tggctggcgt gaccgccgtt gaaacgacca ccagcaggca tccccatatc
    57541 gccaatcagc accgtgccca agcccagatt gctcacctgg ctactcagac gctggataaa
    57601 catcaccaga tccggatgtc cgaaatagcg acgctgatcg gtacgcatga cctgataatg
    57661 ttcggactgt atcggcagcg tgtcagcgcc gacgatacaa ccattagaaa aactgcctat
    57721 cgattgtgcg ctacccggca caggttgggt tattttttgc cacggcgtcg ctgccaggct
    57781 ggcgctactg gcaagcaggg ccagcagcgc aatcgcggtt ttgttcattt tttaccagcg
    57841 tggaatatca gtcttcacat cggcattttg cgcccgctgc cgtaacaggt gatccattaa
    57901 aacgatcgcc agcatcgctt ctgcgatcgg caccgctcgg atcccgacac aaggatcgtg
    57961 acggcctttg gtgatcatct caacttcttc gccaaagcgg ttgattgtgc gccctggcac
    58021 ggtaatgctg gaggttggct tcagcgccat atgggcaatg atttgctgcc cgctgctgat
    58081 accgccaaga atgccgcccg catggttgct ctggaaaccg tctttggtga tttcgtcgcg
    58141 gttctggctg ccacgcagcg ctaccacttc aaaaccatca ccaatttcta cgcctttcac
    58201 cgcgttgatg ctcatcagcg catgggcgat gtcggcatcc aggcggtcaa agaccggctc
    58261 gccaagtccg gcggggacgc cactggcaac aacggtgact ttcgcaccga tggagtcgcc
    58321 ctcttttttc agcgcgcgca tcagttcatc taacgcgtcg attttgtccg ggtccgggca
    58381 gaaaaacgga ttttgctcga cctgcgacca gtctttgatt tccagcggaa tgtcacccat
    58441 ctgggtcagg cagccgcgaa tctcaatgcc aaatttctcg gcgagatatt ttttggcaat
    58501 cgcccctgcc gccacgcgca tggcggtttc gcgggcggaa gagcgcccgc cgccgcgata
    58561 atcgcgcaga ccgtattttt gttcgtaggt gtaatcggca tggcctggac ggaaaacgtc
    58621 tttaatggcg ctgtaatcct gagaacgctg gtcggtattt tcgatcaaca agccaatgct
    58681 ggtgccagta gtaacacctt caaaaacacc ggagagtatt ttgacctgat ccggctcgcg
    58741 gcgctgggtg gtatagcgcg atgtcccagg gcgacgacga tcaaggtcat gttgcaggtc
    58801 cgcttccgtc agcggaatgc ctggcggaac accatcgacg atgcagccga gcgccagccc
    58861 gtgcgattcg ccgaaggtgg ttacgcgaaa gagttgtcca attgtgtttc cagccatcac
    58921 ggctccgtta ttgttgtgtt tgcgtgttta cttaatcttt ataaatcgcg aaatgttcac
    58981 gggccgccag cagctgctct ttggtgagca taaacacacc atcgccgccg ttatcaaact
    59041 ccagccaggt gaacggaaca tccggatatt gttccataag atgtaccatg ctgttgccca
    59101 cttcacaaat cagcacgcca tcatcagcga ggtaatctgc cgcgtttccg agaatgcgac
    59161 gcgtcagttt caggccgtcg gtgccagatg ccaggcccag ttccggctcg tgacggtact
    59221 catttggcag gtcggacata tcttccgcat cgacatacgg cgggttagtg acaatcaggt
    59281 cgtactgcac tttcggtaaa tcacggaaca gatcagagcg aatcggaatg acgttgtgga
    59341 tcagaccgtg ttcttcgatg ttctgttcag caaccgccag cgcgtcggga gagatatcca
    59401 ccgcgtcaac ttccgcatcc gggaaggcat aagcacaagc aatggcgatg cagccgctac
    59461 cggtacacat atctaaaata tgctgcggtt gcttgcttat gagtccggca aatttattgt
    59521 taatcagttc accaatcggc gagcgcggca ccagcacacg ttcatcgacg taaaattcat
    59581 gaccgcagaa ccacgctttg ttggtcaggt aagccaccgg aatgcgttca ttgacgcggc
    59641 ggatcacgcg ttcaacaata cggtgttttt cgctggaggt cagacgcgcg gtgcgcatat
    59701 cttccgggat atccagcggc aggtagagcg aaggcaacac cagctgtacg gcttcatccc
    59761 acgggttgtc agtgccgtga ccgtaccaga tattcgccgc gctgaagcgg ctcaccgacc
    59821 agcgcaacat gtcctgaatg gtttgcagct catttactgc ttcatcaacg aaaattttat
    59881 ccacgtattc ctccagggca tgatcgcaat aatttcggcg gctagtttgc catgaagatg
    59941 acgataaatc agcattcacg cgcggtgagt gaggaaaaat acgtttaaaa cgatcgattg
    60001 cgctacgagt cgggtaaact gtaggaaaat tagaaataga gacagataaa tgaaaaagaa
    60061 aacaacactt agcgaggagg accaggctct gtttcgccag ttgatggcgg ggactcgcaa
    60121 gattaagcag gacacgattg tccaccgacc gcagcgtaag aaaatcagcg aagtgccggt
    60181 gaaacggttg attcaggagc aggccgatgc cagccattat ttttccgatg aatttcagcc
    60241 gttattgaat accgaaggtc cggtgaaata tgttcgcccg gatgtaagcc attttgaggc
    60301 gaagaagtta cgccgtggcg attattcacc ggagctgttt ttggatttgc acggtttgac
    60361 gcagctgcaa gccaagcagg agttaggcgc attgattgcc gcctgtcgcc gcgaacatgt
    60421 gttttgtgcc tgcgtgatgc atggtcatgg gaagcatatt ttgaagcagc aaacgcagct
    60481 gtggctggcg caacatccgc atgtgatggc atttcaccag gcaccaaaag agtacggtgg
    60541 tgatgcggca ttgctggtgt tgattgaagt ggatgagtgg ttgccgccgg aattaccgtg
    60601 aatgctggca tcgaatgccg gatgcggcgt gaatgcctta tccggccgat aaaaatgtga
    60661 taattcaata aattgtgatt catttcagtc ggcgtcacat caggcatcag tgcacaacga
    60721 cgagctataa ctcattcaat acatactgaa aacgacacgt agaatcaatc ggttgccgac
    60781 tgctggtatt caccttcacc acgatatcat gtaactcttt atcggttacc ggatgacgtg
    60841 tgggggtcac cgttaccgtt gatttgtcgc agccataaga ggtaaaggtt aacagttctg
    60901 ataccgcacg gcagtatgca cctctactat caaaaacgct gccgtcacag agtttgccgt
    60961 tttccatcag gtaggtcgaa agcgtggtgg taattgtcga ccccgtggac tgtagctgta
    61021 gcgctttatt atgactgaca taattttcac tccaggagat atcataggag gcgaccggta
    61081 tcgttgggca tattgagtta tgcacagtcg ggctcgtgta gcttccatca aaatatccgg
    61141 gtaagtttgt atatgaataa aaggacgcga tccacgggca accataatta tcagatgcaa
    61201 cggtgacttt aaatgtcata tatgcacaac ggaaaagatc cgtatcaata ccggggcaat
    61261 gtgaattatt aatcgtatca taatccgtta taaccagagt tggtgccggg cccgaacccg
    61321 tccaggaaat cagcctcaga gtatctcctt tacgcccaat agaattatat ttcccatacg
    61381 cgacctcccc tggtgttaac accgtaacat tgaaaacgat actcgcggcc acccctgtta
    61441 ccgtgtccat cgcataataa actctcacac tgtcgaaact tgaactcata agtttatccc
    61501 cagcatagct cctgggtgtg ccgacaacat tcaggacaag cagcatcagg ctcgcgaaaa
    61561 atagggtcca ttttttcatg ttataaatac tcaaaggttg ccgtcacagt ggcgttaaag
    61621 tcaccggacg ttacatttcg cccgctcttc gcctgtaacc aggcattaaa atttaggctg
    61681 ttatttccct gagtcagcgg gagcgtgacg cctgttgtat tgttgactgg cactggtata
    61741 cctgccgctg tttcaattcc aatcccagcg ccggacgcag aagacgaggc gtcaaaagcc
    61801 agaaatccgg gcagttcact atcttctgtt cccgttaacg tgactctgac actcagcgat
    61861 gtagaacttt gacaatcctt caattgaatg accaacggca ctcgctctga ctgcccaatg
    61921 ttcattaaat cgctggcggc aatggcagta aaatgtacct ctgccaactg gctaccgttg
    61981 atcactggtg tacacgattt actgattaaa ttgccggtaa agtgcagatt atctaccgct
    62041 gttgtggtgc cgctgttcag caacagcagg ctattgataa aaatcaacgc acactttttc
    62101 ttcattgata atccacctgt aacgtggccc aggcttcaaa gtccgcctca ggcagatcaa
    62161 caccactctt ttttactggc acagccgtta acaccggtaa atcggtttca ttcaccacga
    62221 gtggcgtatt gatcctaaaa cgctgcccag cctgtttaag ctgaatacct aatccagtga
    62281 tattgctgct tactgcggca tcatcaaaat cgctttgtgt gccactccag ctcaaggtca
    62341 tttccattga tgggtcgcga accgccgtat cacaggtaat tgaatacggc accgtttgct
    62401 cataattact gccattaatg tcatcaacaa acacatcctg aaaggtgatt tctatggttt
    62461 gagcgttgct aatagtgcag ttaggcggct gcactaaata gccgtgaaaa gagacatttg
    62521 agagtgccat cgagcaaaag ccccacagca caagaagtga gatccgtttc attgataatc
    62581 caccacaatt gtggcgctgg cgttaaattc cgctgccgct aaggttgtac cactctgttt
    62641 caccggcacg gcttcaagtg cagggatgcc cgaggaaaaa ttaaacggca accatgaacc
    62701 agacgttaag tcgagtattg agtgatcact acttttttga acccgaatac caaaccctgg
    62761 aatacctgtg ctgataactg tttcaccgtt aatcacgacc gttgtcgcct gcatctgtaa
    62821 acgcaagtca tccattgcca aattgttgca aacaagcgag tagtcaatgg gacggttata
    62881 actcacccca tcgatcttat taatgaatac attaccaaac tcaactgccg caccatttac
    62941 cgtacaagga ggaggggcat caacaagtac ccgtaaattc atacttactg acttactcgc
    63001 tgcaactgca aagccgctac caacacaaca tatcagtaac ccagttaccg cccagacaga
    63061 acaagatcct ttcatactgg ctctcccgtc attagccttt gcggctctgc tcttcatcaa
    63121 cctggcaact attgccgttg caggtgaaaa agagcggcat ccgagcgcca tagtcgttga
    63181 cataggtcag taccggcaca ctccccatct tcacattcag cggtgcagag gttttcggcg
    63241 acatcactat aggcgagaaa ccggctgccg gattgccatt tttctgtgcg ctggcattac
    63301 tgaaaatgac gtaatacggc gtcgggttat tgacagtaaa ttcctgaccg ttgcgggtta
    63361 aggtcacttt atgctgccac gggttttgcg tggtgttttt ttccagagct ttcggtcgcc
    63421 agaaaagttt gatacgcgtc tgaagagcga tttgtagcgt gttggatttg ttcgatttcg
    63481 gcggaatttc gcgcacgttg aaatagaaca tcgattcccg atcggcaggt aatttatgaa
    63541 tatccggcag gccctgaatt ttaacctggc cattcatcat tgcttcaatg cgctgtacag
    63601 gcggcaatac cgccagcggc gagctgattt tgacgccctt ttcatcttca atccagcttt
    63661 gcgccagata aggcagtttg gggtcgttat tacgcagcgt cacgctgata gatttatcgc
    63721 tttcattaaa aatcaggcga gtacgatcgg gcgttactga cgcctgagca gaaaaactta
    63781 ccgttgctgc cagtaacgac agtaccgttg tcaatgtttt ggcagagaga tatttagtca
    63841 tggatcattc cttaatcacg ttattggttc gatgaaacag acatcggcgg ttccattggt
    63901 gtcacctgtt gtgtctgttc ctgaatgacg ggttgaatat catgcggtat gacgggagaa
    63961 cttcccccct tttgctggca tggcaacaac aagccattga acaggtcatt cggtaacggg
    64021 tctggcaggc tgatatcgca gtgagattca ccctcccaga aaacggtcat atgttcaccc
    64081 ggtttaacgc ctgccagata aacattacct tcgtcatcaa ccaaccctac ttgttgttga
    64141 ttatcgtttt ttacttccgc cccaaatggc ggatggctgc catcctgcaa gcgcagcact
    64201 gccatcgcct tctcaccgct gattaccgca aatttacggt agccaatagc gccttccgtc
    64261 agcgttgcct gaaccactga ttttgtagct tcggcgtttt ccggcagttt gttcaaatcg
    64321 atataagcct ggttgcggta gtaatcattc acatctgcca ccaccgcttt gccgaacatg
    64381 ttggtataaa cggcggaacc attaccttca accggtacat tagcaacacc atcagcgtcg
    64441 attaacagac gtgtgccgcc catattttgt gtacgatgca gtgccccgcc atgagcggtc
    64501 aacgttgccc cgccctgtaa agagatccct gctgaggtgt attgcccttc atggtagtta
    64561 gcactgagat caacttttgc cagagtgcca tcgtggctgt agtagccatc cacgctgctg
    64621 tggttttcgc tggttccggc gcttaacgtg tagtggctgg cgtcatcaat gcggtggtac
    64681 aggctcacct gattgctgtc ggcaccgctg ccatagttgc cgttgtaact gatggtgctg
    64741 ttgtcgcccc acggcaaact taaggacaga taaacaccct tgtcggcact tttatcgtat
    64801 tcgtagcgat aaccagtcag agaaacgctc aagttacgaa ggcttcctaa attgaaataa
    64861 tgagaaagca tcatgttgta gttggtttga tcctggcgat cccagtaggt atggtgggaa
    64921 taattgagat agaccgaaac gcgcgcatcc gtgaagttct ggttataggt gaccgtatac
    64981 atctctttgt cgttaccggt gcgcgcccgg tcgtcgtcgt tcgcatccag atactcgctc
    65041 atggtcatgt agtttttttc agagaagcgg tatccagcaa acgtcacccg actgttgagt
    65101 tcatcaaaat ctttggcata gctgacgcga taagagttgc cgtccagcgt tttgttgcca
    65161 tacacgctgt tatcgtcgag ttgaacacga gagtgagtga catcaaacgc taacgcgcct
    65221 aacagcgcca ggtcacgacc gaggcccagc gccgccgact ggtagttttc atcggctaac
    65281 gcaccgccgt agagcgacca gccattggct atcccccacg acgcctcgcc gcccgagaag
    65341 aagctacctg caacctgatg gtcccagttt tgtggacgcc ccattgtcac tttataacgc
    65401 acctggccgg ggcgagtcag gaacggcatg gacgccgtgc tgacgtcgta ttcttgtacc
    65461 tggccgtttt gttcttcaat gcggacatgc agcgtgccag agacggagtc gccaatatcc
    65521 tgaatgcgga atggacctgc gggtacctgg gtttcgtaaa tgacacggcc catttgggtt
    65581 acagtcactt tagcggtagt gtgtgcaaca cctgaaatat cgggggcgta gccgcgcagg
    65641 tttggcggta acatttgatc atcggtactg atgctgccgc caatgtaact gaagccatca
    65701 aaaatatcgg agttgagata atcttcgccc agcgaaagtt tggcttttaa tgacggcaag
    65761 gcgcgccagg cgtagtaacg actccactcc cagttttttt gcgtaccgct atcgtcagtg
    65821 tcgccgtcat cgttactgcg ggtatgctga taatcgctct gccagtcagc tcgtaagcgc
    65881 cacggcccaa cgttaacgcc gaccgtacca ttaccgctga tgtcgttagt gtcatcaccc
    65941 ccattttcat gtcgggtttg cgcgttgata ctgtaatcgg caataagccc agggataccg
    66001 tcatcccagc gcgaaggcgg gtcccactcg ctgtcggtat attccagata agcctgcggt
    66061 acagaaacaa ggagtgcaga ctggcctaaa tcgccgctaa tttctgtacc tgctaaaagt
    66121 gccgtattca gacactgacc atcgcggatc cattgcaaat tctttgcaat atcttccttc
    66181 agaccaaact gggcaaccag ctctggcgat aagcaggcat atgatttatt cggatcgttt
    66241 tcagttgcgt accagtagat atcgtagtcg tcaggtaacg gctgattatt tacatgaacg
    66301 cgtaagttat attttcccgg ttctacatag cctttttgcg aaaaacggcc caaatcaatt
    66361 ttggtattgc cttttaaatc aagaaatcgg ccatcaaact gaatggtatc gtctgccagg
    66421 gcattaaatg gacttccaga aattgccaga gcaatccacc acgaaagccc ccgtaaacgg
    66481 agaaaagaat gttctggcat cggcgtatcc atccatgatt ttttataatt tagttatttg
    66541 gtagttataa accccataac tttgcagcta tgggggaaat acattagaga tacgtgatct
    66601 ggaacgtcgt cagcgtttca aacggtgccg gtgttggtgt tacagcatcc gtttcaccca
    66661 ctaaccatgc tttaaagttc agagtttgtt taggcttgcc ttttcccgtt gaactgtcct
    66721 tcacgatagg ttgcttaatc tctgctccca gtgcataacc tgtaccgtgc tggtcaccga
    66781 taaccaggct aacgtttgga atttcagttc cattatccat gttagacaga gaatagttat
    66841 ctgcgttagt ggagtacgga ttaccggtga atgtggtagc cattgtcgtt tctgtattga
    66901 aattacagtc ctgtaaacga atctggaaat cttttggtgt ggttgttttg ccgccggtta
    66961 aagaacccgt accgatgctg cccatatcca cttccagttt atgatcgtca ggaacaattg
    67021 agcacgggga gtcttcaatt gtgccgtaaa atctcactgt accgttgttc cctgcggcaa
    67081 cgactgatgc tgaagtcagc gcccccatga gcattgccgt ggcaactgct gcttttacaa
    67141 acttactcat tatttgtcct taaatcataa ataattagaa tataagccaa ctgaaatatt
    67201 gacacagagc ttcttcaatt tttacagcga cttttaagca gccaatgaac agattgtcag
    67261 ctcctgaaaa aacaggttcc ttgatataat attctttggc gtgccgaaca tgttgatatt
    67321 ttaatatgac tgatgcaatt tagattttct aacattcaaa gttagaaaaa cttaattaat
    67381 aggcggggat aataattact gaaacttttc atctcaataa tgaagaataa gttcaaaaaa
    67441 acggcaaatg caaaaatcca gtgaataaat taaagtcaaa aacaatcaca tggcgatgac
    67501 tatcttgtga tattaatttc gcaacgatat aatattaaaa cactataatt aatataaaat
    67561 caagacccaa taatattttt cactttagtc attatttacc taaaaatgct tccagaaaaa
    67621 taatttatga ataaaagtaa attctctttc aatatgtaaa tttttacaaa taaaagcgtc
    67681 atgccgtata aaataaagac cattcgatca caagcggatc attgttagta caataagatt
    67741 gcataaattc aacattgcca gaaaaaaata aaggcccgat agattacacc agcgggcctt
    67801 gtgcatgcgt attaaatcgc ttttgccatc ttcagattac acgggctcat ctgccagttg
    67861 aatgtgccgt ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata
    67921 ggcggcgttt cgcccggaca taactcagca accagatacc cgactaaggg taagtgggag
    67981 atcaccagta ccgaagcaac accttcattg gtcaacgcct gtaaataggc actgaccaga
    68041 ccaacatcgc cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga
    68101 caatccccta cttcttccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt
    68161 tcgatttcca ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaaccg
    68221 ttagtagtca gaggacgaac cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc
    68281 atgataaaaa cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat
    68341 ctgaaaccag agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa
    68401 cgctgttttt tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgagcaata
    68461 ctccgccatt cagcgcggat tcatatagct ttgaccttct tattgcaggt cagttgcagt
    68521 tgttttccaa aaactttccc cacgcttgct catctcgact aaacgctcgc aaggggtaaa
    68581 acgggaacca tactgcgtgg ccagtcgttg cattattgca accacttcac ccgcaccgag
    68641 agaatcgatg tagcggaacg gaccaccgag aaatggcgga aaaccaatgc caaataccgc
    68701 gccaatatct ccgtcacgca cgctacggat aacctgctcg tccagacaac gtactgcttc
    68761 attcagcatc aacatcacac accgttcagc tacctgcggt gcggagagtc gcccctgccc
    68821 ttgtgtgcca atcagcgggt aaatggcggg atcgacctgt tttttgcttt tacgcccttt
    68881 ctgaccataa agatagaaac cccggccatt ttttctgcct ttgcgatcgt cgttcaaaat
    68941 tgaagaaaca acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg
    69001 aataatttta gtcccggtgt cgattcctac ctcatccaaa agttggattg ggcctaccgg
    69061 aaaaccaaat ttcaccagcg cggcatcaat gtgctctacc cgttcacctt cggtcaacat
    69121 gcggatagct tcattaatgt aaggcgctaa aatgcgattg acgtaaaacc cggctttgtc
    69181 acgcacgaca attgccgttt taccctgttt tttcgccagt tttactgtgg tagcgatggt
    69241 ttgcgccgat gtccccgcat gaggaataat ctccaccagc ggcatttttt ccaccggact
    69301 gaagaaatgc aggccaataa cttgctcagg tcgcgtagcg tgagcggcga tatcaccaat
    69361 tggtaaagat gacgtattcg aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc
    69421 cgccaccatt tgttgtttta attcgagatt ttcaaacacc gcttcaataa tcagatcgcg
    69481 atgggcaaag ccgcgatagt ccgtcgttcc ggagattaat gccagctgtc tgtcacgttc
    69541 gctggctttg agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag
    69601 cgcatgattt atgccctgcg ggttaatatc tttaattctg accggaagcc ccgctttaca
    69661 agcagtgacg taagcaatac cgccgcccat caagccacca cctaaaattc ccacgctgtt
    69721 taatggcgca ggcggcgcat cactgccggg gtctttcttc acttccgtac tggcaaaaaa
    69781 gatactacgc agcgcctgcg attgcggcgt catcgccagt tcgccaaaag cacgggcttc
    69841 agcgtcatag ccgctactgg tgccttgcgc taatcccgtt tcgacaacct ccaggatgcg
    69901 ttctgtcgcc ggatagttac cctgagtttt gtgttctgtt ttcttgccga ccattttgaa
    69961 cagcagcgca cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcgtga
    70021 agatggacga tcctgctttg ccagctcaac agcggcttcc agtaaaatgg attgcggaac
    70081 gacgtcatcc accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag
    70141 gatcatctct aatgctgtgc tgacgcctat cagacgcggt aaacgctggg tgccgcctga
    70201 accgggtaat aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt
    70261 acacatgcga ccgtggcacg ccagcgccaa ctccagccca ccgcccaggc aagcgccatg
    70321 aatagccgcg ataaccggaa cgggcaaagc atgaatctcc gccattaact gttgaccctg
    70381 ccgcgccagt gcttccgctt cttgcgccgt tttgcagttg gcgatcatgt tgatgtctgc
    70441 gccagcaatg aagttgtctg gtttcgcgga gataaacacc acgccgcgca actctttgtt
    70501 ttcacggagt tgcttaataa tggcgcgcac ctgcgaggca aactccgcct tcagggtatt
    70561 cattttctca cccggtacgt cgatggtgat aacagcaatg ttgtccagac ggacattaag
    70621 ggtaaacgct gatgccattt ccattattcc gcctccacaa ccattgccgc gccaagccca
    70681 cccgcagcac aggcggtcac taatccaaat ccaccgccgc gacgccgaag ttcatgcaat
    70741 gtctgggtaa tcatccgcgc gccggtcgcc gcgaagggat gcccgtaagc aatcgaaccg
    70801 ccaagcacgt taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagtaca
    70861 tcacgagcaa aacgttcgct acccagcaac tgaatattcg ccagtgtctg agctgcaaag
    70921 gcttcgtgca tatcgatcaa tgtcagatcg ctcattgtca aaccggcacg ttccagcgcc
    70981 agcggcgttg accaggccgg accgagcaac atgtcctgcc agacatctat cgcagtaaat
    71041 gcgtaactgc gcagataccc cagcggtacc agccctaatt ctttcgcccg cgattcggtc
    71101 atcaggatca ccgccgctgc gccatcggtc agcggcgtac tgtttgctgc cgttaccgtc
    71161 ccgtgtttac gatcaaacgc cggacgcaac tttgcgtaat cagcaagcga ggaattaccg
    71221 cgaatattgt tgtcttcgac aagcggttgt ttataaggag ggataaaggc agtcatcacc
    71281 tcttctttga gtttaccgtc tgaccacgcc tgagcggctc gctgatgcga acggtgcgct
    71341 aatgcatctt gctgttcgcg agtaataccg taggttttcg ccatttgttc agcggtgtcg
    71401 cccatccgca agccagtaga gtattccgcc actgccggag gcaccggcat taagtcgcgc
    71461 aaacgcaggc gagagaagag tttcaggcgc tggctcatgg ttcgagcttt gttgacatca
    71521 accagcacgc gcgccagttt tttactgacg ccaattggca atacggaaga ggaatcggcc
    71581 ccaccggcaa tccccgcgcg aatagttccc gccattaggc tttctgcgac gtttgcaatc
    71641 gcctggaaac tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt
    71701 ccagtaccga gaacaatttc acgcgcaatg ttgggggctt ccggcatttg tacgacctga
    71761 ccaaaaacca gttgttcaat cacttcggcg gggatctcgc tgcgtgccag caattcgcct
    71821 accaccatct tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctggcgggca
    71881 aaaggcgtac gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt
    71941 aaaacctgac ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat
    72001 agtcttaacc atttttttac atttagccaa gcggagaaaa gggaaagtgg gagctatgac
    72061 acagagagaa aagtagaaga gaaaataaaa cgcccctgcc aacaactgac aggggcgtac
    72121 gtacccgaga ggaattaacg cagacccagc tggaagatca gcatttcagc ttcgcaggca
    72181 aaagtgaaat cgatatccag gcgcacaccg tcagactctt cagtgaaagt cggggtgatt
    72241 ttgcacggtt cggattccac gctacgggct ttttcagtca gcgccgccag cgtttgttct
    72301 gcttctgcgc ggtttgcaaa cacgcggctg taagacgcgg tgcagtcgga gttgtccata
    72361 atggtgccaa catccataca gcagcaaacc ggggtttcat cagcactaca tttactcatc
    72421 gttgatttcc tctgtatgtg cacccaaggt gccagataaa cgttgtggat attttacgct
    72481 tccggaaagt gctgctccag ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca
    72541 cttaaaaatg atctaaaaca aaattcaccc gaatccatga gtgcgccacc tccaaatttt
    72601 gccagctgga tcgcgtttct tagatcatat ttgaaaaaag atagaaacat acttgcaaca
    72661 ttccagctgg tccgacctat actctcgcca ctggtctgat ttctaagatg tacctcagac
    72721 cctacacttc gcgctcctgt tacagcacgt aacatagttt gtataaaaat aaatcattga
    72781 ggttatggtc atgagccaga aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc
    72841 acttatctcc acccaggcct ggtcggcagg ctttcagtta aacgaatttt cttcctctgg
    72901 cctgggccgg gcttattcag gggaaggtgc aattgccgat gatgcaggta acgttagccg
    72961 taaccccgca ttgattacca tgtttgaccg cccgacattt tctgcgggtg cggtttatat
    73021 tgatccggat gtagatatta ccggacattc tgatttaacc ggtcagagtg caaatgctaa
    73081 aaatatcgca ccgacagcgt gggtacctaa cctgcacttt gtcgcgccta tcaacgatca
    73141 gtttggttgg ggagcgtcaa taacttccaa ctacggcctg gctacggaat ttaccgacaa
    73201 ctacgctgct ggtatctatg gcggtaaaac cgatctgcag acaatcaacc ttaaccttag
    73261 cggggcttat cgccttaaca atagctggag ttttggtctt ggttttgatg cggtttacgc
    73321 caaagcaaaa attgagcgtt acgccggttc gctggggcct atgttgtctc agcagttgat
    73381 tgcccggggt gtacccgctt cattaaccaa tggtattaat actccggact ctcaaattgc
    73441 ccatctgaaa ggtgacgaat ggggcttcgg ctggaacgcg ggtattcttt atgaactgga
    73501 taaagataac cgttggggcc tgacatatcg ttcagaagtt aaaatcgact tcgaagggga
    73561 ttacaaaagt tccattaacc caaatctgaa caatattttg ggtagcatgg gcttaccgta
    73621 tggcacgatg ggtgcaacac aaaatggctc cctgacgctc aacctgcctg agatgtggga
    73681 gatttctggt tataaccgtg ttgcgccgca gtgggcaatt cactacagca tggcctatac
    73741 cagttggagc cagttccagg agttgaaagc gaaaggcgat agtggccaga ctctgttcta
    73801 taaagatgaa ggattccgtg acgcatatcg tatcgcgttg ggcacaacct actattacga
    73861 taaaaactgg accttccgta ccggtatcgc ctatgacgat agcccagttc cggcagataa
    73921 acgttctatt tccattcctg accaggatcg cttgtggtta agtgctgggt taacctacgc
    73981 atttaatgaa gatgcatcca ttgacgttgg cgcatcttat atgcatggtc agaaagtgaa
    74041 gtttactgaa ggtgaaggac cggcagctta ttccttcgag tctgaaggta aagcctggct
    74101 gttcggtact aactttaact acgcgttctg ataacgcgtt
//
LOCUS       DAOTRK010000008        69130 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.008, whole genome shotgun sequence.
ACCESSION   DAOTRK010000008 DAOTRK010000000
VERSION     DAOTRK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.008"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            86..241
                     /locus_tag="QIE41_000567"
     CDS             86..241
                     /locus_tag="QIE41_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDS3529495.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            401..619
                     /locus_tag="QIE41_000568"
     CDS             401..619
                     /locus_tag="QIE41_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529496.1"
                     /translation="MQKREPVIIVPDYTDDELYEWIHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            642..1016
                     /locus_tag="QIE41_000569"
     CDS             642..1016
                     /locus_tag="QIE41_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529497.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            1557..1745
                     /locus_tag="QIE41_000570"
     CDS             1557..1745
                     /locus_tag="QIE41_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor"
                     /protein_id="HDS3529498.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            1742..1930
                     /locus_tag="QIE41_000571"
     CDS             1742..1930
                     /locus_tag="QIE41_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HDS3529499.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            2023..4461
                     /locus_tag="QIE41_000572"
     CDS             2023..4461
                     /locus_tag="QIE41_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDS3529500.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSVQEKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHSSEKPVTHVAREEL
                     QALHIARAEELPAVTALAISHKTKLLDPLEIRDLHKLVRDTDKVFPNPGNSDLGLITA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTFDALD
                     VEIAAATLPMDFNIYEIPGSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINGGLA
                     AECKTEEEKNDEDNPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTADEV
                     NKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQESEQND
                     QKAEQNSPNALQNEPETKQPEPVVQQEPEKICTACGQTGGGNCPDCGAVMGDATYQET
                     FDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHHVITS
                     TSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SVIWQKLIPSQADF"
     gene            4523..4792
                     /locus_tag="QIE41_000573"
     CDS             4523..4792
                     /locus_tag="QIE41_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDS3529501.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            4761..5879
                     /locus_tag="QIE41_000574"
     CDS             4761..5879
                     /locus_tag="QIE41_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HDS3529502.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKVYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            6046..6840
                     /gene="potC"
                     /locus_tag="QIE41_000575"
     CDS             6046..6840
                     /gene="potC"
                     /locus_tag="QIE41_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDS3529503.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            6837..7883
                     /gene="potD"
                     /locus_tag="QIE41_000576"
     CDS             6837..7883
                     /gene="potD"
                     /locus_tag="QIE41_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDS3529504.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            7941..8402
                     /locus_tag="QIE41_000577"
     CDS             7941..8402
                     /locus_tag="QIE41_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDS3529505.1"
                     /translation="MSQDSRVFFHIFLGIGLVLILISVVVFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            8399..9184
                     /locus_tag="QIE41_000578"
     CDS             8399..9184
                     /locus_tag="QIE41_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDS3529506.1"
                     /translation="MKKIIILLSLLILLPLTATSNPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHASSSLSDVKGSVLLLFTTRE
                     PRLIMITNGQVESGLDDKHLGLLVENHTQAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDPPGSTTILGLFAWAVAFIVFTAFFYYTTRLNYALKFAVAMTIA
                     NMGYQALCLYIDNSLAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(9322..10143)
                     /gene="cobB"
                     /locus_tag="QIE41_000579"
     CDS             complement(9322..10143)
                     /gene="cobB"
                     /locus_tag="QIE41_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDS3529507.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(10159..11070)
                     /gene="nagK"
                     /locus_tag="QIE41_000580"
     CDS             complement(10159..11070)
                     /gene="nagK"
                     /locus_tag="QIE41_000580"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDS3529508.1"
                     /translation="MYYGFDIGGTKIALGVFDSVRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADKRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(11099..12343)
                     /gene="lolE"
                     /locus_tag="QIE41_000581"
     CDS             complement(11099..12343)
                     /gene="lolE"
                     /locus_tag="QIE41_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDS3529509.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(12343..13044)
                     /gene="lolD"
                     /locus_tag="QIE41_000582"
     CDS             complement(12343..13044)
                     /gene="lolD"
                     /locus_tag="QIE41_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDS3529510.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(13037..14236)
                     /gene="lolC"
                     /locus_tag="QIE41_000583"
     CDS             complement(13037..14236)
                     /gene="lolC"
                     /locus_tag="QIE41_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDS3529511.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            14497..15570
                     /locus_tag="QIE41_000584"
     CDS             14497..15570
                     /locus_tag="QIE41_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDS3529512.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLSTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            15698..19144
                     /gene="mfd"
                     /locus_tag="QIE41_000585"
     CDS             15698..19144
                     /gene="mfd"
                     /locus_tag="QIE41_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDS3529513.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSVKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            19288..20250
                     /gene="ldtC"
                     /locus_tag="QIE41_000586"
     CDS             19288..20250
                     /gene="ldtC"
                     /locus_tag="QIE41_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDS3529514.1"
                     /translation="MMIKTHFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(20333..20590)
                     /gene="bhsA"
                     /locus_tag="QIE41_000587"
     CDS             complement(20333..20590)
                     /gene="bhsA"
                     /locus_tag="QIE41_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDS3529515.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            20831..21463
                     /gene="comR"
                     /locus_tag="QIE41_000588"
     CDS             20831..21463
                     /gene="comR"
                     /locus_tag="QIE41_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDS3529516.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDHFAAKHEAQLFCEEKSVETALADYFA
                     AVANCFTSKDTPAGCFMINNCTTLSPDSGDIGNTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(21525..22064)
                     /locus_tag="QIE41_000589"
     CDS             complement(21525..22064)
                     /locus_tag="QIE41_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDS3529517.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDGNGQLILNNKV"
     gene            complement(22291..23595)
                     /gene="ndh"
                     /locus_tag="QIE41_000590"
     CDS             complement(22291..23595)
                     /gene="ndh"
                     /locus_tag="QIE41_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDS3529518.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(23926..24468)
                     /locus_tag="QIE41_000591"
     CDS             complement(23926..24468)
                     /locus_tag="QIE41_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS3529519.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(24491..25516)
                     /gene="nagZ"
                     /locus_tag="QIE41_000592"
     CDS             complement(24491..25516)
                     /gene="nagZ"
                     /locus_tag="QIE41_000592"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDS3529520.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVCQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(25527..26351)
                     /gene="thiK"
                     /locus_tag="QIE41_000593"
     CDS             complement(25527..26351)
                     /gene="thiK"
                     /locus_tag="QIE41_000593"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDS3529521.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(26332..26973)
                     /gene="lpoB"
                     /locus_tag="QIE41_000594"
     CDS             complement(26332..26973)
                     /gene="lpoB"
                     /locus_tag="QIE41_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDS3529522.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(26987..27364)
                     /locus_tag="QIE41_000595"
     CDS             complement(26987..27364)
                     /locus_tag="QIE41_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDS3529523.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(27367..27726)
                     /gene="hinT"
                     /locus_tag="QIE41_000596"
     CDS             complement(27367..27726)
                     /gene="hinT"
                     /locus_tag="QIE41_000596"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDS3529524.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            28060..30249
                     /gene="fhuE"
                     /locus_tag="QIE41_000597"
     CDS             28060..30249
                     /gene="fhuE"
                     /locus_tag="QIE41_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDS3529525.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIVLALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(30309..31742)
                     /gene="ptsG"
                     /locus_tag="QIE41_000598"
     CDS             complement(30309..31742)
                     /gene="ptsG"
                     /locus_tag="QIE41_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDS3529526.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(32036..32833)
                     /locus_tag="QIE41_000599"
     CDS             complement(32036..32833)
                     /locus_tag="QIE41_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDS3529527.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(32844..33848)
                     /gene="holB"
                     /locus_tag="QIE41_000600"
     CDS             complement(32844..33848)
                     /gene="holB"
                     /locus_tag="QIE41_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDS3529528.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHYGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(33845..34486)
                     /locus_tag="QIE41_000601"
     CDS             complement(33845..34486)
                     /locus_tag="QIE41_000601"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDS3529529.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(34476..35498)
                     /gene="yceG"
                     /locus_tag="QIE41_000602"
     CDS             complement(34476..35498)
                     /gene="yceG"
                     /locus_tag="QIE41_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDS3529530.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVSSERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(35501..36310)
                     /gene="pabC"
                     /locus_tag="QIE41_000603"
     CDS             complement(35501..36310)
                     /gene="pabC"
                     /locus_tag="QIE41_000603"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDS3529531.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSILNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVIPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(36430..37671)
                     /gene="fabF"
                     /locus_tag="QIE41_000604"
     CDS             complement(36430..37671)
                     /gene="fabF"
                     /locus_tag="QIE41_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDS3529532.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(37759..37995)
                     /gene="acpP"
                     /locus_tag="QIE41_000605"
     CDS             complement(37759..37995)
                     /gene="acpP"
                     /locus_tag="QIE41_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDS3529533.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(38206..38940)
                     /gene="fabG"
                     /locus_tag="QIE41_000606"
     CDS             complement(38206..38940)
                     /gene="fabG"
                     /locus_tag="QIE41_000606"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDS3529534.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(38953..39882)
                     /gene="fabD"
                     /locus_tag="QIE41_000607"
     CDS             complement(38953..39882)
                     /gene="fabD"
                     /locus_tag="QIE41_000607"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDS3529535.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(39898..40851)
                     /gene="fabH"
                     /locus_tag="QIE41_000608"
     CDS             complement(39898..40851)
                     /gene="fabH"
                     /locus_tag="QIE41_000608"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDS3529536.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(40919..41989)
                     /gene="plsX"
                     /locus_tag="QIE41_000609"
     CDS             complement(40919..41989)
                     /gene="plsX"
                     /locus_tag="QIE41_000609"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDS3529537.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(42070..42243)
                     /gene="rpmF"
                     /locus_tag="QIE41_000610"
     CDS             complement(42070..42243)
                     /gene="rpmF"
                     /locus_tag="QIE41_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDS3529538.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(42295..42816)
                     /gene="yceD"
                     /locus_tag="QIE41_000611"
     CDS             complement(42295..42816)
                     /gene="yceD"
                     /locus_tag="QIE41_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDS3529539.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            43015..43599
                     /gene="yceF"
                     /locus_tag="QIE41_000612"
     CDS             43015..43599
                     /gene="yceF"
                     /locus_tag="QIE41_000612"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDS3529540.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(43711..44670)
                     /gene="rluC"
                     /locus_tag="QIE41_000613"
     CDS             complement(43711..44670)
                     /gene="rluC"
                     /locus_tag="QIE41_000613"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDS3529541.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            45243..48428
                     /gene="rne"
                     /locus_tag="QIE41_000614"
     CDS             45243..48428
                     /gene="rne"
                     /locus_tag="QIE41_000614"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDS3529542.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPAVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVKPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            48561..48854
                     /locus_tag="QIE41_000615"
     CDS             48561..48854
                     /locus_tag="QIE41_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDS3529543.1"
                     /translation="MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            48851..49345
                     /locus_tag="QIE41_000616"
     CDS             48851..49345
                     /locus_tag="QIE41_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS3529544.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIGR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(49440..50393)
                     /gene="flgL"
                     /locus_tag="QIE41_000617"
     CDS             complement(49440..50393)
                     /gene="flgL"
                     /locus_tag="QIE41_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDS3529545.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50405..52048)
                     /gene="flgK"
                     /locus_tag="QIE41_000618"
     CDS             complement(50405..52048)
                     /gene="flgK"
                     /locus_tag="QIE41_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDS3529546.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(52114..53055)
                     /gene="flgJ"
                     /locus_tag="QIE41_000619"
     CDS             complement(52114..53055)
                     /gene="flgJ"
                     /locus_tag="QIE41_000619"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDS3529547.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTAAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53055..54152)
                     /gene="flgI"
                     /locus_tag="QIE41_000620"
     CDS             complement(53055..54152)
                     /gene="flgI"
                     /locus_tag="QIE41_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDS3529548.1"
                     /translation="MIKFLSALILLLVTTAVQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54164..54862)
                     /gene="flgH"
                     /locus_tag="QIE41_000621"
     CDS             complement(54164..54862)
                     /gene="flgH"
                     /locus_tag="QIE41_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDS3529549.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKS
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(54915..55697)
                     /gene="flgG"
                     /locus_tag="QIE41_000622"
     CDS             complement(54915..55697)
                     /gene="flgG"
                     /locus_tag="QIE41_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDS3529550.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(55834..56589)
                     /gene="flgF"
                     /locus_tag="QIE41_000623"
     CDS             complement(55834..56589)
                     /gene="flgF"
                     /locus_tag="QIE41_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDS3529551.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(56609..57814)
                     /gene="flgE"
                     /locus_tag="QIE41_000624"
     CDS             complement(56609..57814)
                     /gene="flgE"
                     /locus_tag="QIE41_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDS3529552.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLVFNANGVLTSDPTANISTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(57839..58534)
                     /gene="flgD"
                     /locus_tag="QIE41_000625"
     CDS             complement(57839..58534)
                     /gene="flgD"
                     /locus_tag="QIE41_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDS3529553.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58546..58950)
                     /gene="flgC"
                     /locus_tag="QIE41_000626"
     CDS             complement(58546..58950)
                     /gene="flgC"
                     /locus_tag="QIE41_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDS3529554.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(58954..59370)
                     /gene="flgB"
                     /locus_tag="QIE41_000627"
     CDS             complement(58954..59370)
                     /gene="flgB"
                     /locus_tag="QIE41_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDS3529555.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59526..60185
                     /gene="flgA"
                     /locus_tag="QIE41_000628"
     CDS             59526..60185
                     /gene="flgA"
                     /locus_tag="QIE41_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDS3529556.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60261..60554
                     /gene="flgM"
                     /locus_tag="QIE41_000629"
     CDS             60261..60554
                     /gene="flgM"
                     /locus_tag="QIE41_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDS3529557.1"
                     /translation="MSIDRTSPLKPVSTVQPRENTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60559..60975
                     /gene="flgN"
                     /locus_tag="QIE41_000630"
     CDS             60559..60975
                     /gene="flgN"
                     /locus_tag="QIE41_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDS3529558.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(61015..62550)
                     /gene="murJ"
                     /locus_tag="QIE41_000631"
     CDS             complement(61015..62550)
                     /gene="murJ"
                     /locus_tag="QIE41_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDS3529559.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62660..63583)
                     /locus_tag="QIE41_000632"
     CDS             complement(62660..63583)
                     /locus_tag="QIE41_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS3529560.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKKQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(63585..64232)
                     /locus_tag="QIE41_000633"
     CDS             complement(63585..64232)
                     /locus_tag="QIE41_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDS3529561.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPVVTAMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(64243..64827)
                     /gene="rimJ"
                     /locus_tag="QIE41_000634"
     CDS             complement(64243..64827)
                     /gene="rimJ"
                     /locus_tag="QIE41_000634"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDS3529562.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65063..66271
                     /gene="mdtH"
                     /locus_tag="QIE41_000635"
     CDS             65063..66271
                     /gene="mdtH"
                     /locus_tag="QIE41_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDS3529563.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66335..66982
                     /gene="grxB"
                     /locus_tag="QIE41_000636"
     CDS             66335..66982
                     /gene="grxB"
                     /locus_tag="QIE41_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDS3529564.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            67116..67676
                     /locus_tag="QIE41_000637"
     CDS             67116..67676
                     /locus_tag="QIE41_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDS3529565.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            67782..68828
                     /gene="pyrC"
                     /locus_tag="QIE41_000638"
     CDS             67782..68828
                     /gene="pyrC"
                     /locus_tag="QIE41_000638"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDS3529566.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGGMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRNGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            68902..>69130
                     /gene="dinI"
                     /locus_tag="QIE41_000639"
     CDS             68902..>69130
                     /gene="dinI"
                     /locus_tag="QIE41_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDS3529567.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADD"
BASE COUNT        17136 a        18263 c        17237 g        16494 t
ORIGIN      
        1 gaacatgacg gcagggttca actttaataa ctatgcagca ggtttttgtt ccgctacccc
       61 ggcgttaagg ggaaatgagg tcagcatgga tactatcgat cttggcaaca gcgaatctct
      121 ggtatgtggc gtgttcccca accaggacgg tacgttcacc gcgatgacgt ataccaaaag
      181 caaaacgttt aaaaccgaaa atggtgcccg tcgctggctg gaaagaaact caggtgagtg
      241 atatggattt caacacaatc atggaaaagg cttacgaaga atacttcgaa ggccttgccg
      301 aaggcgaaga agccctcagc ttcagtgagt ttaaacaggc gctttccagt tcggcaaaat
      361 ctaacggctg ataagcgaaa cagcaccgcg aggaatcagt atgcagaaac gagaacccgt
      421 catcatcgtg ccagactata ccgatgatga actttatgag tggatacacc agaaaattaa
      481 ggctgcgcag gacctgaaat gggccaatga agccagggct aagcaggctg aaaatctgtc
      541 cgctctggag caggatatca ccaggctgga aaaagcagcg gcattaagca ttgccagaat
      601 gattacatac ccgcgttaat agctaaccaa cgaggctaat aatggaattt aaagatttac
      661 caatgccatt ccaggaaatg gcagcgaatg tggttcgctc tcaactggcg actcttgacc
      721 tgagtaccgt agaaaaagaa accatcgaca atatatccgg taacgtacgc cgagccttta
      781 tcgggctgta cgaagagaag cagctctctg ataaccagga tttacatgaa aaatactttc
      841 tggaattaat ggacatcatt aataaaggat ttggcttgtt aatgaaaaag aaagggattc
      901 gaatagctcc cctcgaaaat cattttacag caagcagtat taattcctgt gatttaaagc
      961 atcacacatc cgatgggaaa gttgaatcaa acaacaaaat atcaattaat cattaattta
     1021 ttcacaggtg aggtagagtg cgtgcgccgg acacggataa gaatccggca ctgacagttt
     1081 actgaaagga tatttccatg aaaagtcaga ccataacgcg aaagcgcacg gcgaggtagc
     1141 tggttcatag atagcctgtc gttaaatttt cgtcgaccgt gcgcttccgg ttgtgacact
     1201 ccgcgaaatg gcgcggcggt aagtatgacg gggttattcc ttccccgttg aggacaccgg
     1261 gttgtcaggt tgaccatacg cttaagtgac aaccccgctg caacgccctc tgttatcaat
     1321 tttctggtga cgtttggcgg tatcagtttt actccgtgac tgctctgccg ccctttttaa
     1381 agtgaatttt gtgatgcggt gaatgcggct atgcgcacgc ggaacagtta aagcagtaag
     1441 gcggtctttt actggcgtaa cgagcatcaa ctaacccggc gttaattgtt aactggttaa
     1501 cgtcacctgg aggcaccagg cactgcatca caaaattcat tgttgaggac gcgataatgg
     1561 aaacgttatt accaaacgtt aatacgtctg aaggttgttt tgatattggt gttctgctca
     1621 gtaaccggga gtttactgaa gatgccatta atatgaggaa atatgagcct tatctgctca
     1681 atgataattc catactttcc cgaattgctc ttcttgaact tggtattttc ggagagcgtc
     1741 aatgacttca gcatttgcac tgatgatgac ggtttttctt ataacgggtg aatcacagaa
     1801 tgtgattacc ggaatttatg ccagtaaaga atcctgcctc caggcaagag acgagcaaaa
     1861 aatttctggt gaatgcctcc cggtaaaaaa agtatcgctg tacctgaata acgaaacacc
     1921 ggctggataa ccctccagcc atattaacac cataccaacg gattaaaaat gccagcaatg
     1981 gcagggattt gttcaccctt aaatctgtaa tgaggtttat caatgagcac tgataaagaa
     2041 gaatttgcgc tatattgcga agcaaaaaat gacaaagtca gaaaacgtct gggaattaaa
     2101 ggtggttttt actggactac agcaaaaaaa ttatctgttg ccatctcccg ctgcattacc
     2161 gcaatggatg acaacgatta tgatgaagac gactttaaaa aacccgttcg cgtccattta
     2221 cccgttgtga atgaccttcc acctgaaggc gtgtttgata ccgaattctg caaccgatac
     2281 gaaaaaggcg gggaagatgg cattacaatg gtatttatcg cgccctctcc ctccgtgcag
     2341 gagaaaccag ccagtactga caataccaac gtcaacggcg aagacatgac ggagattgag
     2401 gagaatatgc tcctgccggt ttctggtcag gagctgccca ttcgctggct tgcgcaacac
     2461 agcagcgaaa aaccagtaac gcacgttgca cgggaagaac ttcaggcatt acatatagca
     2521 cgggcggaag aactgccggc tgttactgcc ctggccattt ctcacaaaac aaagctgctc
     2581 gacccgctgg agattcgcga ccttcacaaa ctggtacgcg acactgacaa agttttccct
     2641 aatcccggta attcagacct gggactgata actgcttttt tcgaggcata cctggacgct
     2701 gattacactg atcggggtct gctgacaaaa gagtggatga aaggaaatcg tgtttcgcgt
     2761 atcacccgta cggcttccgg tgcaaatgcc ggtggcggga acaaaaccga tcgcaatccg
     2821 aatttagtac acaccttcga tgcgctggat gtggagattg cagcagccac acttccgatg
     2881 gattttaata tttatgaaat tccgggcagc gtttatcgtc gcgcaaaaga aatcgtcctg
     2941 aaaaaagaaa gtccgttcag ggaatggtcc gcagcacttc gcgcaacccc tggtatcctg
     3001 gactattccc gcgcagctat ttttgcactt atccgaagcg cccaccctga gttttatcac
     3061 tacccggggc gccttcaggg gtatatcaat gccaacctga cagaaactga tcacgagaat
     3121 cccacagctg aaacgctcat ggctgcccgg catacaccgg aaaaagatat cctggaagaa
     3181 attaacggcg gactggctgc tgagtgcaaa acagaagaag aaaaaaatga tgaagataac
     3241 ccgcaaccat ctggcgcaat ggcagatgaa caggcaacgg ctgaaacaat ggcagcggat
     3301 acagttgaac atcatcagga cccgcagccg ctggatgaca agtcacaggt aaaagttacc
     3361 gctgacgaag taaacaaaat tatgcaggca gccaatatca gccagcctga cgccgataag
     3421 ttgcttgctg cctctcgcgg agaatttgtt gcagggatta gcgacccgaa tgatccgaaa
     3481 tgggtaaagg ggattgaaac ccgcgattct gtaaaccaga accagcaaga atcggaacag
     3541 aacgaccaga aagcggaaca aaacagccca aatgcgttac aaaacgagcc agaaacgaaa
     3601 caacctgaac cagtagtgca acaggaaccg gaaaagatct gcaccgcctg cggtcagacc
     3661 ggcggcggca actgccctga ttgtggcgcg gtgatgggcg acgcaacata ccaggaaaca
     3721 ttcgatgaag agaatcaggt tgaagttcag gaaaatgatc cgaaggaaat ggaaggcgct
     3781 gaacatccac acaaggagaa tgctggcagc gctcaggatc acgccagcga tagtgaaact
     3841 ggcgagacgg cagatccctt aattacggtg aacggtcatc atgttatcac atccaccagc
     3901 aggacgtgtg accatctaat gatcgacctt gaaaccatgg gaaaaaatcc tgatgccccg
     3961 atcatctcaa taggtgcaat atttttcgat ccgcaaaccg gagatatggg accggaattt
     4021 agtaagacta tcgatctgga aactgctggc ggggtcattg atcgggacac cattaaatgg
     4081 tggcttaagc aatcacgcga agcgcaatct gccattatga ccgatgaaat cccgttagat
     4141 gatgcactgt tacaattgcg ggaatttatc gacgaaaact ccggtgaatt ttttgttcag
     4201 gtctggggaa atggagccaa cttcgacaac acgattttgc gccgttcata cgaacggcag
     4261 gggatcccct gcccgtggcg ttactacaac gatcgagatg tacgcacaat cgttgagctg
     4321 gggaaagcca tagacttcga tgccagaacg gctattccat tcgaaggtga gcgccataat
     4381 gcacttgatg acgcccgtta ccaggcaaaa tacgtttcag ttatctggca aaaactgatc
     4441 ccgagtcagg ctgattttta atgttcaacc gtcgccagtt gtcgttgata ttctgcaact
     4501 ggcgcgttcc ggagtgatag ccatgagcga acagtacctg ataacgctcg acgagtggaa
     4561 accaaaacgg ttcagtctcc caataacaaa cactaccctg gtgaaatacg gaaaactagg
     4621 atacatcgtt ccaagaccac aaaaaattcg tgggcgttgg ctgatagatc gccgagcagt
     4681 atttgttggg cctggtgaaa cgggaattgc gccggaaatt catactggcg atgatgatgc
     4741 actgaaggag attttaactc atgtcaccga ggccacgaaa aaacagcact gacgtagccg
     4801 gtctttacga aaagtttgat cgcagaactg gcagagttta ctaccagtat aaaaatcctg
     4861 tgactggaaa atttcacgga ctcggaacag acaaaggtaa ggcagaaaaa atcgcttcca
     4921 cagccaatca gcgaatagct gcagcagaag ctgaatattt catgcgcaaa attgatgaaa
     4981 gtccgtcagc aacaaaacgt cggggtatca gattaaaggc atgggttgat cgatatctga
     5041 aaatacagga cacgcgactg aaaaatggag atattgcagc tacaactcac aaagaaaaaa
     5101 ctcgaatggc tgcatacctg gtttcccgtc tgggaaacca cccattgaaa gaactggaag
     5161 taagagactt tgcattaata ctggatgagt ggctggataa agacatggtc agcacagcga
     5221 gagtaaatcg tggattatgg gttgatattt ataaagaagc acagcatgca ggggaagttc
     5281 ctcctggatg gaatcctccg gaggctaccc gtaaaccgat ccctaaagta accagagcca
     5341 ggctcaccat ggaagactgg caaaaagttt acaatgcaac gcctgaaaaa cactttatcc
     5401 gtaacgcaat gcttcttgcg attgttactg gtcagcgccg tgatgacatt tgccacatgc
     5461 gtttttcaga tgtgtggaac gaacacttgc atatcaccca gggaaaaacc ggaatgcgtc
     5521 tggcgttacc gcttacacta cgctgtgatg ccattgggat aacgttaaaa gaagttattg
     5581 atgggtgccg agacagaata ttaagtccat atctaatcca tagtcggcac cagaaacaac
     5641 cgaagccgat gagtaaagac aacctgagcg actactttgc caaagcacgg gatctggctg
     5701 ggataattcc accagcagga aaaactccgc caacatttca tgaacaacgc tctctatcag
     5761 aacggctgta ccgtgcacag ggtatcgata caaaaacatt actaggacat aaagtccagg
     5821 caaccaccga tcgctataac gatactcgag gtcaggaatg ggttaagttg gttatttgac
     5881 aaacaccata tggaatatcc gtcaccatct caggaaaaaa gtgttgtaaa atgcgtggcc
     5941 tagttttggg gaaacgtttt ggagagattt tggagaaagg aaaaaattga ataaattcaa
     6001 aatcctgaaa gtgtcccgcc ttatgaataa aaaggtggaa ctcgaatgat cggtcgactg
     6061 cttcgcggcg gttttatgac cgctatctac gcgtacctgt atatcccaat cattattttg
     6121 attgtgaact cctttaacag ctcgcgcttt ggcatcaact ggcagggttt taccaccaaa
     6181 tggtatagcc tgctgatgaa caacgacagc ctgttacagg cagcgcagca ttcactaaca
     6241 atggcggtgt tttcggcgac gtttgctacg cttatcggtt cactgacggc agttgcgctg
     6301 taccgttatc gctttcgtgg taagccgttc gttagcggaa tgctgtttgt ggtgatgatg
     6361 tcaccagata tcgtgatggc gatttcgctg ctggtgctgt ttatgctgct gggtattcag
     6421 cttggcttct ggtcgctgct gttctcgcat atcaccttct gcctgccatt tgtggtggtg
     6481 acggtgtact cgcgcctgaa aggttttgac gtgcgaatgc tggaagcggc gaaagatctc
     6541 ggtgccagcg aatttaccat tctgcgaaaa atcattctgc cactggcaat gccagcggtg
     6601 gcggcgggct gggtgttaag ctttaccctg tcgatggacg atgtggtggt ttcttcgttc
     6661 gtcaccggac caagttatga aattctgccg ttaaaaattt attcgatggt taaagtcggc
     6721 gtatcgccgg aagttaacgc gctggcaacc atattactgg tgctgtcgct ggtgatggta
     6781 attgccagcc agcttattgc tcgtgataaa acgaaaggta acacagggga cgttaaatga
     6841 aaaaatggtc acgccacctg ctcgcggcgg gtgctctggc actgggcatg agcgccgctc
     6901 acgccgatga caacaacacg ctgtatttct acaactggac cgagtacgtg ccgccaggac
     6961 tgcttgaaca gttcaccaaa gaaaccggta ttaaggttat ctattcgact tacgagtcga
     7021 acgaaaccat gtacgcgaag ctgaaaacgt acaaagacgg tgcctatgac ctggtggttc
     7081 cttcaaccta ttacgtcgat aaaatgcgta aagaagggat gatccagaag atcgacaagt
     7141 cgaagttaag caacttcagc aacctcgatc cagacatgct caacaagccg tttgacccga
     7201 ataacgacta ctccattccg tatatctggg gggcgacggc gattggcgtt aacggtgatg
     7261 cggtagatcc gaaatccgtt accagctggg ccgatctgtg gaagcctgag tacaaaggca
     7321 gcctgctgct gaccgacgat gcccgtgaag tgttccagat ggcgctgcgc aaactgggct
     7381 actccggtaa cactaccgat ccgaaagaga ttgaagctgc atataacgag ttgaaaaaac
     7441 tgatgccaaa cgtcgcggcg tttaactccg ataaccccgc taacccgtac atggaaggcg
     7501 aagttaacct cggcatgatc tggaacggtt ctgctttcgt tgcacgccag gcgggtacgc
     7561 caattgatgt ggtgtggccg aaagaaggcg gcattttctg gatggacagc ttggcgatcc
     7621 cggcaaatgc caaaaacaaa gaaggcgcgc tgaaattgat caacttcctg ctgcgcccag
     7681 atgtggcaaa acaggttgca gaaactatcg gttatccaac gccaaacctt gcggcgcgta
     7741 agctgttaag tccggaagtg gcgaacgata aaacgctcta cccggatgct gaaaccatta
     7801 aaaatggtga atggcagaat gacgttggcg cagccagcag catttatgaa gagtattatc
     7861 agaagctgaa agcaggacgt taattcagca agcgggcggt ggtattaccg cccgtttttt
     7921 atttagcaag gatgtggcta atgagtcagg acagtagagt tttctttcat atattcctcg
     7981 gtatcggtct ggtactaatc ctcatttctg ttgttgtctt ttataaccag ttcacctata
     8041 gcaaagatgc gattcatact gaaggcgtca ttgttgatac cgtctggcac tccagtcatt
     8101 ctcatcgcac gggtaaaaat ggttcgtggt atcccgtcgt tgcctttcga ccgacgcctg
     8161 attacacgct aattttcaat tcgagtattg gcagcgattt ttatgaagac agcgaaggcg
     8221 ataaagttaa tgtttattat tctccaggac atcctgaaaa ggctgagata aataacccgt
     8281 gggttaattt ttttaaatgg gggtttatcg gcattatggg cgttattttt atctccgtcg
     8341 gtttattaat cagtatgccc tcctctaaaa agacacgcag gaaacggaag tcccgcccat
     8401 gaaaaaaatc ataatattgc tcagtttact gatactcctg cctctgacgg caaccagtaa
     8461 tccattgatt ccaataatga agactctttt tacagatgtt acaggtactg taccggatgc
     8521 tgaagagatc gcgcataaag ccgagctttt tcgccaacaa accgggattg cgccgtttat
     8581 cgtggttgta cctggcgtta atgaagcaag cttcaggcaa aatggcaaag cgatgctggc
     8641 gcatgcgtca tcttcattga gtgatgtaaa aggcagcgtc ctgttacttt ttaccacccg
     8701 cgagccacgg ttaattatga taaccaacgg ccaggttgaa agcggtctgg acgataaaca
     8761 tctcggcctt ctggtagaaa atcacactca ggcttattta catgcggacc tctggtatca
     8821 gggaatcaat aatgcattgg ctgttttaca agcacagata ttaaaacaac cgacgccgcc
     8881 actgacatat tatccgcatc ccggacaaca gcaggagaat gatcctcccg gcagtaccac
     8941 gatattaggt ctttttgcct gggcagttgc attcatcgtc tttactgcat ttttttacta
     9001 cacgacgcgc cttaattatg cattaaagtt tgcagtggcg atgactattg ccaatatggg
     9061 ttatcaggct ttatgtttgt acatcgataa tagcttggcg attaccagaa tatcgccgct
     9121 ctgggcaggg ctgattggtg tctgtacgtt tattgccgcg ttgttattga cgagtaaacg
     9181 gtaaaagagg cagtaaagtg tgcgtttatt atgccggatg cggcgcgaac gccttatctg
     9241 gcccacaaaa cccgcaaatt caattaattg cgtccccttg taggcctgat aagcgtagcg
     9301 catcaggcaa tgcttcctgc tttataagcc cttcagcaac ttttcaacaa actctggcac
     9361 cacctggctt gccgggccgt aatatttctc ggcaaattca ttaccgacct ggcttggttc
     9421 aagattcagt tccacggtgt gtgcgccatg cagtttcgct tcgtgaacaa acccagccgc
     9481 cggataaaca tgccctgatg taccaatggc aatgaaaata tcggccattg acaacgccat
     9541 ataaatttca tccatgccga gtggcatttc gccaaaccaa acgacgtgcg ggcgcaaggg
     9601 tgccgggaac tggcaacaat ggcatttatc ttctggcgta acgtctccgg tccagtcgag
     9661 aacctgacca ctttgtgaac aacgaacttt cagcagttcc ccatgcatat gaataacatt
     9721 ggtgttaccg gcacgctcat gaaggttgtc gatattctgc gtcaccagca aaaagcgatc
     9781 gccgagggca tcttgcagtt tagccagcgc aagatgcgcg gcgttaggct gaatttctgg
     9841 ctgctgtagc tgtcgacgac gggcgttata aaacgcttgc accagttcag gatcgcgatc
     9901 gaaaccttcc ggcgttgcca catcttcaac ccgatgttct tcccacaggc catctgcggc
     9961 gcgaaaggta cgaatacctg attccgcaga aatacctgcc cctgtcagta cgagtactct
    10021 tggtttttcc attgcttccg gcaccacttt atctctgaaa aaaatacgct gacgcaaacg
    10081 ctcgcgcaga cggcgtttat ttttacgaaa acgacttaac cgatgacccc gacgcgacag
    10141 catagcaacc tctgttgttt aatcggttag atgtaggaag gccgcaccac gcattcctcc
    10201 cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga gctacaggta agagatgacg
    10261 aggcagcctg tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc caccaccaat
    10321 gacgaccagg tcagggtcaa caatggtcag gatatttccc agacaaaccg ctaataaatc
    10381 cagataacgc tcaacgtgcg cccttgcctg ctcatcacct tgatcataaa gcgcaataat
    10441 ttcgggagcc tgcaacggtt gatgataata gtgttgatac agccacgcaa aaccgcgacc
    10501 agacagataa ttttcaatgc agccatgctg accacagccg cagcggcgta acgggaaatc
    10561 cagccccatc atggttaacg catcaaccgg cagacgcata tggccaaact cgccggtaat
    10621 atagctttta ccggtaattg gtttaccgtt gaaaatcagc ccgccgccaa cgccggtgcc
    10681 gagaatcaac cccatcacca gtggatattg agtaaattca tcatcccagg cttctgaaag
    10741 ggcaaaacag ttggcatcgt tatcaaggcg gacatcgcga tcaagacgtg cgctcaggtc
    10801 ggcacgcagc ggtttaccgc tagcagcggg gacattggca gcatacagcg taccatcttc
    10861 tgtttccggc attcccggaa taccgatgcc gacagaacct ttacagccaa aacgcttatc
    10921 ggcttcagcc accagctcgc acactgcatc taaaaatgcg tcatagctgt cacgcggtgt
    10981 cggcacccgc ttttcccact gcaactgccg aacgctatca aacacgccaa gcgcaatttt
    11041 cgttccacca atatcaaacc cgtaatacat tgccgctcct tgttttaatg tactgccttt
    11101 actggccgct aaggactcgc gcagggtcaa tattactggc gcgccgcgcc ggataccaac
    11161 ttgccaaaag actcagcaac aatgctgtga ccagtacgta gaagacgtcc agccagtgca
    11221 attccgatgg caggaagtca ataaaataga tatcgctgga gaggaactga tgaccgatca
    11281 acttttcaat ccactcaata atcggggtaa gttgcagtga aacaactacg ccgataatca
    11341 caccacacag gctaccgaat agccctgcca gcaatccata ccagacaaag atggcgcgaa
    11401 ttaaaccatc tttcgccccc agcgttctta atactgcgat atcgccactc ttgtctttca
    11461 ccgccatcac taaggtggag acgatgttga aacaggccac gccaatcacc agtaccatcg
    11521 ccagatacat aatggcgcgg atcatctgga tatcgcgata catatagccg taagtaccaa
    11581 tccaactttt aatataaaca tagctgttgg tcacttcacc cgcatcgcgt accagcttat
    11641 tggcgttgaa aacatccgtc attttaaggg caatacctga cacgctggaa cccatatcaa
    11701 gatattgttg ggcatctgcc agcgggatca tggcaaaact gtgatcgagt tgaccactca
    11761 actgcaaaat accggcaatg tgcaaacgca cacgttttgg ctgcatcagt ttatgctcgg
    11821 gattcgagtt ggggatcata atcgacaccc aatcgccctg cttcactttc agcgcatccg
    11881 ccacgccttt gccgatgata atttgctgtt cgcccgcttt aaaattgcgc caggcatccc
    11941 cctgaacaaa cgagggtaat gcgctcagac gctgttcctg ttgcgggtta acgcccttta
    12001 cctggattgc gcgaagattc gcgccacttt ctaccagccc ggtgaaattg atatacggcg
    12061 cagcggcggc aatacccggc actttctgca ctttatccag tgcttcctgc cagttagtcc
    12121 acggctgatc caccgcttcg atttcaccat gcggcaccac cgccagaatg cggttattca
    12181 gttcgcgttc aaagccgttc atcgcgctta agccgacgat caataccgcc acgccgaggg
    12241 caatgccaat ggtagaaatg acggagatca gcgacaccat gccgccgcgc cgccgtccgc
    12301 gactaaaacg caggccaatt aataacgata aaggcatcgc cattactccg cccccatcag
    12361 gctcagttcc gccgtcagac gaccatcacg catctccagt tggcggctca tacgtttcgc
    12421 cagttgcagg tcgtgagtaa ccaccaggaa ggcggtgccc tgcaaacgat tcaactcccc
    12481 aagtaactgg aagatgctgt cggcgttacg cgcatcaagg ttaccggtag gttcatccgc
    12541 cagtaccaga cgcgggttat tgaccagcgc acgggcaatc gccacacgct ggcgttcgcc
    12601 accagaaagt tcagatgggc ggtgattcgc acgatgctcc agccccaccg cttttaacat
    12661 ctccagcgca cggctgttga tttcggcggg ctttttcttg ccaatcagca gtggcatagc
    12721 cacgttttcc agggcagtaa aatccggcag caggtggtga aactgataaa taaagcccag
    12781 cttctggttg cgcagttctg ctttcgccgc tgacgacagt ttgctcatcg gctgaccgtt
    12841 aaagatcaca tcgccggagg ttggcgtatc cagcccgccc agcaggtgca gcaaggtgct
    12901 tttaccggaa ccagagctac cgacgatcgc catcatttca ccttcgccga cgctgaaact
    12961 gacattgtgc agtacatcag tttgcacact gccttcctga tagcgtttgc acaggttgtc
    13021 gcattgcaac aggatcttat tcataacgta aagcctcagc gggttgtgtg gcggcagcgc
    13081 gccatgaagg gtaaagcgta gacagcagcg cgatagccat cgccaccagc gcaataacaa
    13141 tgacctgtaa aggttcgata gcgaccggca gcgccgcgcc atcaagcagg acgccgatta
    13201 tcggcatcag attatttaac tggctggcaa gcagtgcgcc aagcgccgct ccgaggattg
    13261 caccgataat cccggcgctg gccccttgca ccataaagac catcatgatt tgccgcggag
    13321 tcagcccctg agtctgaaga atggccactt cgccctgctt ctccatcacc attaacccca
    13381 gcgaggtaat aatgttaaac gccgcaacgg cgacaatcag gctcagcagc aagcccatca
    13441 tatttttttc catgcgtacg gcctggaaca gctcgccttt acggtcacgc cagtcctgcc
    13501 atttgctgcc ttcaggcagt ttttgttgac ttaatgagtc gactttcagc ggctcatcca
    13561 gccacaatcg ccagccggta atattgcctg ccggataacg catcagacgc gaggcatcct
    13621 caatattcac cagcatttca tagccatcga cttcactgtt ggcagcgaaa gtgccaatca
    13681 cattgaacag acgctggctt ggaatacgcc ccatcggcgt gaactggctg gcagatggca
    13741 ccatcacgcg gatttgatca ccgcgattga cgcccaactg tgaggcaagc tgttcaccga
    13801 ggatgacatt atatttcccc ggctcgaggt cagtttgttt cacattgacc agatacggcg
    13861 ttagtggatc tttttgcgcc ggatcgatac ccagcatcac cccgaccgcc acgctgcgcg
    13921 cgctttgcag taccacatca ccggtagtaa taggtgcgac gcgattaacg ccgtccagtt
    13981 tgactgccgt ttccgggagt tgctgcgggt taagagagcc atgctcagaa gagagaattg
    14041 cctgtggcat caggccaagg atgttgtttt gcagctcgcg ctcaaaaccg ttcatcactg
    14101 acaatactgt gaccagcgcc atcaccccga gggtaatgcc gatggtagaa agccaggaga
    14161 cgaaacgacc gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca ataaatagag
    14221 cgacaggttg gtacatgaaa tccgtctggt tgctgtagca aagacacgag tatataagcg
    14281 aaagcgggat gggtaaacgt ctgaactgta agtgatcgtc agggctaatc tgaaaagtag
    14341 cagcaaatca atcgactaaa gggcctcatt ttctcattgg ccttgtggcc tgatctttat
    14401 ctgaaaaata accaaataca gacagatacg cattacacca cgcgactggc ccctcaggat
    14461 caacagcatg ttaaataaaa agagcgtgtt gtcctgatga aacaaaaaga gctatggatt
    14521 aaccagatca aaggattatg tatttgtctg gtagtgattt atcactcggt gattaccttt
    14581 tatccgcatc tgagcacttt tcagcatccg ttatcggaag tcctgagcaa atgctggatc
    14641 tatttcaatc tttaccttgc accctttcgt atgccggttt ttttctttat atccggctat
    14701 ttgattcgcc gctatatcga cagcgtaccg tggggaaatt gtctcgataa acggatctgg
    14761 aacatcttct gggtgctggc gctttggggc gtggtgcagt ggctggcgtt aagtgcgctg
    14821 aaccagtggc tggcacctga gcgcgattta agcaatgcct ccaatgccgc ttatgccgat
    14881 tccaccggtg agttcctgca cgggatgatc accgccagca ccagcttgtg gtatctgtat
    14941 gcgttaattg tctatttcgt ggtatgtaaa atttttagcc gtctggcgct gccactattc
    15001 gccttgtttg tactgctgag tgtggcggtt aatttcgttc ccacgccgtg gtggggaatg
    15061 aacagtgtga tccgcaattt gccttattac agccttggcg catggtttgg cgcaacatta
    15121 atgacctacg ttaaagaggt gccgttgcgc cgccatctgc tgatggcttc tttgctgacc
    15181 gttctggcgg tcggtgcctg gttgtttact atctcgctgc tgttgtcgct ggtatcgatt
    15241 gtggtgatca tgaagctgtt ttatcaatac gagcaacgtt tcggtatgcg ctccaccagc
    15301 ctgctgaatg tgattggcac caacaccatt gctatctaca ccacccatcg cattctggta
    15361 gagatattca gcttaactct gcttgcgcca atgaacgcgg cacgctggtc gccgcaagtc
    15421 gaactgacac tcctgatggt ttaccccttt gtcagtttgt tcatctgtac tgttgcgggc
    15481 ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc tgttgttctc cccgccttca
    15541 ctgcccgctg ccgtcagtta ctcccgctaa gccatcgccc cagccggggc gattcaattt
    15601 gctaaaccat gtcgattacg gataatgaac agcattgctt atcagagata tgcccccata
    15661 tgttgaggca tatcctaacg agaatctgac aaccgttatg cctgaacaat atcgttatac
    15721 gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag ttaactggcg ctgcctgcgc
    15781 gacgctggta gcggaaattg ccgaacgtca cgccggacca gtggtgctta ttgcgccaga
    15841 tatgcaaaat gctctgcgtt tgcatgatga aattagccag tttaccgacc agatggtgat
    15901 gaatctggcg gactgggaaa ctcttcccta cgacagtttt tcgcctcatc aggacattat
    15961 ctcctcgcgc ctttccaccc tttaccagct accgacgatg caacgtggtg tactgattgt
    16021 tccggtgaat acgcttatgc agcgcgtttg cccgcacagt tttctccacg gccatgcgct
    16081 ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta cgaacccaac tggacagcgc
    16141 cggttatcgc catgttgacc aggttatgga gcacggcgaa tacgccacgc gcggcgcgtt
    16201 gctggatctc ttcccgatgg gaagtgagct gccttatcgt cttgatttct ttgatgatga
    16261 aatcgacagt ctgcgggtgt ttgacgtcga cagccagcgc acgctggagg aagtagaagc
    16321 gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa gcggcaattg aactgttccg
    16381 cagccagtgg cgcgatacct tcgaagtgaa gcgtgatccg gaacatattt accagcaagt
    16441 gagtaaaggc acattacctg cggggatcga gtactggcag ccattgttct tcagcgaacc
    16501 actgccgccg ctgttcagtt atttccctgc caataccttg ctggtgaata ctggcgatct
    16561 ggaaaacagt gccgaacgtt tccaggctga cacgctggcg cgttttgaga atcgcggcgt
    16621 cgatccgatg cgcccactgt tgccaccaca atcgctctgg ctgcgggtgg acgagctctt
    16681 ctcagagctg aaaaactggc cccgagtgca gctaaaaact gaacatttac cgacaaaagc
    16741 cgcgaatgcc aatttaggtt tccagaaact gccagacctg gccgttcagg cacaacaaaa
    16801 agcgccgctg gatgcgctgc gtaagttcct cgagtctttc gacggtccgg tggtgttctc
    16861 ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg ctcgcgcgaa ttaaaattgc
    16921 tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt gggcgttatc tgatgattgg
    16981 cgctgccgaa catggttttg tcgatacggt gcgtaatctg gcgctgatct gcgaaagcga
    17041 tctgctcggt gaacgcgttg cccgtcgtcg tcaggattct cgccgcgcca tcaaccccga
    17101 tacactgatc cgtaacctcg cggagctgca tattggtcag ccggtggtcc atctggagca
    17161 cggtgtcggt cgttatgcag gaatgaccac gctggaagct ggaggcatta ctggcgagta
    17221 cctgatgctc acctatgcca acgacgccaa actgtatgtt ccggtatcgt cactgcatct
    17281 gattagccgt tacgcaggtg gcgcggaaga aaacgccccg ctgcataaac ttggcggcga
    17341 tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg cgtgatgtgg cggcagaatt
    17401 actggatatt tacgcgcaac gcgccgccaa agagggcttc gcgtttaaac acgatcgcga
    17461 gcagtatcag ttgttctgcg acagcttccc gtttgaaacc acgccggatc aggcacaggc
    17521 cattaatgcg gtacttagcg acatgtgtca gccgctggca atggatcgtc tggtgtgcgg
    17581 cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc gctttcctgg cagtagataa
    17641 ccacaagcag gtggcggtgc tggtgcctac cacccttctc gcgcagcagc attacgacaa
    17701 cttccgcgac cgtttcgcca actggccggt acgtatcgaa atgatctccc gtttccgcag
    17761 cgtcaaagag cagacgcaaa tccttgcgga agtggcggaa gggaaaatcg atattctgat
    17821 cggtacgcac aaactgctgc aaagtgacgt caagtttaaa gatttaggcc tgctgattgt
    17881 cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc attaaagcga tgcgtgcgaa
    17941 cgtggatatt ctgacgctta ccgctacgcc gatcccacgt acgctgaata tggcgatgag
    18001 cggaatgcgc gatctgtcga ttattgccac gccgcccgcc cgtcgtctgg cggtcaaaac
    18061 gtttgtccgc gagtatgaca gtctggtggt ccgggaggcg atcctgcgtg aaattttgcg
    18121 cggaggacag gtttattatc tctacaatga tgtggaaaac atccagaaag ccgccgaacg
    18181 gctggcagaa ctggtacctg aagcgcggat tgccatcggt cacgggcaga tgcgtgagcg
    18241 cgaactggaa cgggtgatga atgatttcca tcatcaacgt ttcaacgtgc tggtttgtac
    18301 caccattatc gaaaccggga tcgacatccc gacagccaac accattatca ttgaacgcgc
    18361 ggatcacttc ggtctggcgc agctgcacca gttacgcggt cgcgtcggac gttcgcatca
    18421 tcaggcatat gcatggttgc tgacaccgca tccaaaagcg atgactaccg atgcacaaaa
    18481 acgtcttgaa gcgattgcct cgctggaaga tctcggggca ggttttgcgc tggcaacgca
    18541 cgatctggag attcgcggcg cgggtgaact gcttggcgaa gaacaaagcg gctcaatgga
    18601 aaccatcggt ttctcgctgt atatggagtt gctggaaaac gccgtcgatg cgctgaaagc
    18661 cggacgcgag ccgtcgctgg aagatctcac cagtcaacaa acagaagtcg agctgcggat
    18721 gccgtcgctg ttgccggatg atttcattcc tgacgtgaat acgcgtttgt cgttctataa
    18781 acgtattgcc agcgccaaaa cggaaaacga actggaagag atcaaagttg agcttatcga
    18841 tcgcttcggc ctgctgccgg atccggcgcg taccctgctg gatattgccc gtctgcgcca
    18901 gcaagcgcag aaactgggga tcaggaagct ggaaggtaat gagaaaggcg gcgtgatcga
    18961 atttgccgag aagaatcacg ttaatccggc atggctgatt ggtttgctgc aaaaacagcc
    19021 gcagcattat cgcctcgatg gcccgactcg cctgaagttt attcaggatt tgagcgagcg
    19081 aaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa ctggaagaga acgcgatcgc
    19141 ttaatagtaa atcgggggcg tttctgcgcc cccatctgca acatttacaa attctttgca
    19201 cttccctgca ctatccgaca ctgtcaccat ccataattca gactgatctt tttattacaa
    19261 taaccttata tttattatgg atttttggtg atgatcaaaa cgcatttttc tcgctggcta
    19321 acgtttttta cgttcgccgc tgccgtggcg ctggcgctac cggcaaaagc caacacctgg
    19381 ccactgccgc cagcgggcag tcgtctggtt ggcgaaaaca aatttcatgt ggtggaaaat
    19441 gacggtggtt ctctggaagc catcgccaaa aaatataatg tcggctttct cgctctgtta
    19501 caggctaacc ccggcgttga tccttacgtg ccgcgcgcgg gtagcgtgtt aacgatcccg
    19561 ttgcaaaccc tacttccaga tgcgccgcgc gaaggcattg tgatcaatat tgcggaactg
    19621 cgtctctatt actacccgcc gggtaaaaat tcggtaaccg tgtatcccat cggcattggt
    19681 cagttgggtg gtgacacgct gacgccaacg atggtgacta ccgtttcgga caagcgtgca
    19741 aacccaacct ggacgccaac agcgaatatt cgcgcccgtt ataaagctca ggggattgag
    19801 ctacctgcag tggttccggc aggaccggat aacccaatgg gccatcatgc gattcgtctg
    19861 gcggcctatg gcggtgttta tttgcttcac ggtacgaacg ccgatttcgg cattggcatg
    19921 cgggtaagtt ctggctgtat tcgtctgcgt gatgacgata tcaaaacact ctttagccag
    19981 gtcacccctg gtaccaaagt gaatatcatc aacaccccga ttaaagtctc tgccgaacca
    20041 aacggtgcgc gtctggttga agtacatcaa ccgctgtcag agaagattga tgacgatccg
    20101 caactgctgc caattacact gaatagcgca atgcaatcat ttaaagatgc agcgcaaact
    20161 gacgctgaag tgatgcagca tgtgatggat gtccgttccg ggatgccggt ggatgtccgc
    20221 cgtcatcaag tgagcccaca aactctgtaa atcctggata aaaaaaatcc ccgcgacacg
    20281 gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat gagggttaat gcttatttat
    20341 aaattactgc tgttccatgg agggtatttg gaccggttac agaagtaata cggaatgatt
    20401 ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc cagcgatccc agatttgtcc
    20461 ccgcgttagc actgatagta ccgacttttt gttggccttc tggcgttgac tgaacttcga
    20521 cagccgcaaa gctggcaaat gacattgagc ttaaaatcgc cgcagcgatg agggttttta
    20581 cgtttttcat aatagtggcc ttatgcagat gaatgacgtc gcaagcaatt acttaacgat
    20641 cgataactaa atgatagatg tgatctggat cacatacaag atatttttta taacaatcat
    20701 taattaattt aaaaatcttt aaatttcagt taaatagaaa gcaattattt ttatgggatc
    20761 tggactggtg aacggaggtg attattttta taataacctt tcattcacag tcaccaggta
    20821 caacggcatc atggcaactg actcaacaca atgtgtaaaa aaaagccgtg gccgcccaaa
    20881 agtgttcgac agggatgccg cgcttgataa ggccatgaaa ttgttctggc aacacggtta
    20941 tgaagcgact tctcttgcgg acctcgtcga agcgaccgga gccaaagcgc ccacgctata
    21001 cgcggaattt accaacaaag aggggttatt tcgcgctgta ctcgaccgct atatcgatca
    21061 ttttgccgct aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agactgcgct
    21121 ggctgactat tttgctgccg tcgccaactg ctttaccagc aaagacaccc cggctggctg
    21181 ctttatgatc aacaactgca ccaccctctc cccggattcc ggagatatcg gcaatacgct
    21241 gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc
    21301 gcgcggggaa atcccgaccc actgtgacgt gacgcatctg gcagaattcc ttaattgtat
    21361 tattcagggg atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac tgatgcagat
    21421 tgcccgaacg actttgcgtt tatggccgga actggtgaaa taaggcgcaa aaaaagcccc
    21481 tcaacctgag cgaatgaggg gctaaattgc agagtacagc cttgttatac tttgttattc
    21541 aaaatcagtt gcccgttgcc atctaacggg atctgcgtac ccggatcgcg gtccatgcgg
    21601 attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac
    21661 ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga
    21721 gagccctgga tctggttacc ggcatagcca cctcccagcg cccccacaac agtggcgaca
    21781 tctttaccgc gaccaccgcc aaactgatgc cctatcacgc cgccagcaac agcgccgagc
    21841 accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtgacgttg
    21901 cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga
    21961 gcgtattgcg ggccacgttc aaacacgttc agactggcca ctgccgctac gcccaacgca
    22021 gctgcgacac caatcccgat acccgccaac attgatttat tcacgggaac acctccttct
    22081 ttgctgttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa
    22141 agcgcgggaa tggctggaaa tacttaggga ttcggaggag tctgaaggaa tgcctgatgc
    22201 gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggataaggc
    22261 gtttacgccg catccgccag tacaagccga ttaatgcaac ttcaaacgtg gacggataac
    22321 gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat
    22381 ctggtgcatt cggtatagcg agatgtatac aaagcgcgca attcgtcctt caatcatcat
    22441 tgagccgcgc gtcaggttac ccatcaggct accgacggtg gagaagttcg acagcgatac
    22501 cagagaaccg tgatctttat actgataatt tttcagcggc ttaccgttca tctgcgccag
    22561 aatgttgttc attgcgcagg ttgccatctg gtgtgcggcc tgcgcacgcg gcggaacaaa
    22621 gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtatatgt ctggatcgcg
    22681 agtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttggttt caagaccacc
    22741 gatatctttc aggaagtctg gtgctttgat cccggctgcc cacaccatca gatcagcctc
    22801 aatatattca ccatctttag tatgcaggcc gccttcatca gcactggtga ccatggtttg
    22861 cgtcagcacg cgaacgccaa gtttcgttaa ctcattatgg gccgcagcag agatacgtgg
    22921 cggtaatgca ggcaaaatac gttcgcccgc ttctaccagc gttacgttca gggcttcgtt
    22981 ggtcagacct ttataaccgt agctgtgcag ttgcttgacc gcgttgtgca gttcagcgga
    23041 gagctctaca cccgtcgcgc cgccgccgac aatcgcaatg ttcactttgc cattcgcgcc
    23101 caggttggcg gagtatttca ggaacagatt cagcatctcc tggtggaagc gacgcgcctg
    23161 gtgcgggtta tcgaggaaaa tgcagttctc tttgacacct ggcgtattga aatcgttaga
    23221 ggtgctaccc agcgccatta ccagggtgtc ataggcgatt ttacgttccg gaaccagcaa
    23281 ttcacctttt tcgtcgcgca gttctgcaat agtgattgtt ttcgcttcac gatcaatatc
    23341 aattacggaa cccagctgga actggaaacc atgattgcgc gcatgggcca gatagctcaa
    23401 cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagcg gtttccacag
    23461 gtggctgtgg ttacgatcga ccagcgtgat tttggctttt ttcttgcgtc ccagcttatg
    23521 ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca caattttttt
    23581 caatggcgta gtcaacgtga cccccttaaa tttattaacc aattgttaat taatggttat
    23641 tggcatagct cttaattaac aacaggttac agcaatgaaa atgttcttcg ctattgagaa
    23701 taacatgaat ggtgcattgg tcataccaaa attgatgtta atcaagtttt gttgctgaaa
    23761 agataggcga ctttaccggg cgagatcaaa aaacagagtt tgttgtttat tatttgagct
    23821 aatttatgag agtggtatag cgtgaggggt gtatggagga cgtttggtag gcctgataaa
    23881 atgcgcatca ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt
    23941 gaacgcttta atgcgctgta aatgcgggga gatattcttg aatttgtgcg tctgctcttc
    24001 gtcccagaca atctcgtaat aatgatgcag ctcttcagat gtccgctggc tgttaagcgc
    24061 ttcatcatta cgcgacaaaa tcaccaggca acgatcgcga ttcttttcgc ggaagttggt
    24121 cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt
    24181 ctcataaggg aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat
    24241 ccgttccgcc cagtatccgc ccagaccaac gccgcaaatc agcggacgct cgtcgacgtt
    24301 cagttgcaac attttgtcca cttctttgag cagatgctgc atatcatgtt tcggatgccg
    24361 cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc
    24421 gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca
    24481 taagccaggg ttagtgacct gctttctctt cctgccagcg ttcatgtaac tgattcagac
    24541 gggcgctgat cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt
    24601 tatgatacaa acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga
    24661 cggccccttt acgattattg cagaccagga tcatatcgca acccgcgtcc agtgatgcct
    24721 gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga
    24781 aaatcacgcc atcaaaaccc agttcctgac gcaaaacggt tttcagccaa tagggagaac
    24841 cgctcgccgg acgcggatcg acatcactgt agatcacatg cgcaggcata atggcgtcga
    24901 gtttattttc gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt
    24961 gcggacgtgg gtcacacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg
    25021 ggaagtgttt cccggtagtt ttcattccgg cttcatgcat accatcaata aaccggctgg
    25081 caattgccag ggctttttcg ggatcggcat gataagaacg ctcgccaatc gccgcgctga
    25141 tatgcccgac gtccagcact ggcgcaaagc tgatatcaat atccatagca atcatttcgc
    25201 tggccatcaa ccaacctgcc tcttgcgcca gttttccgcc ttcttccatt cccaacagcg
    25261 cagcaaatga ttgtgccgct ggcaagcggg taaaaccttc acgaaaacgc tgcacgcgtc
    25321 caccttcctg atcaaccgcc accaccagat gattgcgcga agctgcgcgg atctggcaca
    25381 ccagttcacg taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca
    25441 ccagcggatg cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca
    25501 acattactgg acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca
    25561 tcggccagcc tgataaattg ttgatcgccg gtttgtcgcc aacggtactc aaaccaccct
    25621 gctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga
    25681 taaatcttcg cgcgagtggc atagtcattg accaattgcc ggtgctggtc agtattttcc
    25741 acccacaccg ccgccagttc cagcgcgata tcaccatctc ctgcatactc ccagtcgatg
    25801 agttttaacc ctgacgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga
    25861 cttaagcgta aaggccgtgg ttcccgcgct ttgcgcagac gttttaacct tcgcagccaa
    25921 cccactgtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa cggcaacagc
    25981 gttattcgcc agccaaaacg cggttgttga tgtagataat acagcaagcc tgccagttcg
    26041 ttggtatccg gcaaatacgt ttttacctcg ccgggcagat agtcgaccac catccagtca
    26101 cggagatata aatgcggctt cggtgcaatg catgcgggta gttgtgataa agcccgatac
    26161 tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc
    26221 tggccctgat gttcaatgag aaaactcccg ccactaagcc cactattaaa cgtcgtgacg
    26281 ggatgaaatt gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattgctg
    26341 cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat
    26401 ttgtagggtc ggagcgttaa cgttgccaga ggcactggag tatagcacgt aatgagcgcc
    26461 gacattgcgg gcaatgccta tggctttact acgggtgcct aaactgtcct gcggcgacaa
    26521 acctaactgt tgctttgcca tcgacagctg ctgggcggaa accagggtaa atttaccgtt
    26581 attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt
    26641 acggttgtta acgctatcaa ccagcaggac gctacccgca gtcacgccgt cagccccaag
    26701 catcttactg accatcggct gcattgcacc attccagtca taatggcgaa tatgcggcgc
    26761 aggcggtgca gtttgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac
    26821 cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac
    26881 ttcttctacc ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc
    26941 ggtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat
    27001 aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat
    27061 gtgcgggaat ggtaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt
    27121 cataccagta aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg
    27181 aggatgctga aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg
    27241 cagccaataa agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat
    27301 gtgaacgaca gcccaccagc actaacaacg ccagagacac cagcccaaag catccttttc
    27361 tcatcgttac agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg
    27421 catatggatg tggtaaacct cttgtccgcc gtggcggttg gtgttcatga tcagacgata
    27481 gccatcttcg gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatgcgtcc
    27541 cagcgcctgc tcatgctcag cagacacgtc gttcacagtc ggaatgagga tattcggaat
    27601 gatcagaata tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc
    27661 ctggtagacg atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc
    27721 tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca
    27781 ctgcgagtat agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct
    27841 taagcaaaaa atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa
    27901 tgcgctgaaa agacattctc tgtttggttt acacgccaaa catgaatgcg tatatttctc
    27961 atttgcattt acatacaaaa ttattcgcac actaaatata accgctcatc accctcctcc
    28021 ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac
    28081 agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg cattgtactg
    28141 gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag
    28201 ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgca
    28261 ggtaccaaaa tgcagatgac tcaacgtgat attcctcagt cggtcactat tgttagccag
    28321 cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg
    28381 atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt attcccgcgg attccagatc
    28441 gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa tctgggcgac
    28501 gcactttctg atatggcact gtttgaacgc gtagaagtag tgcgtggcgc gacaggactc
    28561 atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt
    28621 cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg
    28681 gcggatttac aaagcccact caccgaagat ggtaaaatcc gcgcgcgaat tgtcggcggc
    28741 taccagaata acgactcatg gctggaccgc tacaacagtg aaaagacctt cttctccggc
    28801 attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc
    28861 attgatgtta atagcccaac ctggggcggt ttaccgcgct ggaatactga tggcagcagc
    28921 aacagttacg atcgcgcacg cagtaccgca cctgactggg cgtataacga taaagagatc
    28981 aacaaggtct ttatgaccct caagcagcgg tttgctgata cctggcaagc gacactgaat
    29041 gccacccact ccgaagtcga atttgacagt aaaatgatgt atgtcgatgc ctatgtaaat
    29101 aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt tgattatgtc
    29161 ggcggcaccg gttggaacag cggcaaacgt aaagttgatg cgctggattt gtttgctgac
    29221 ggtagttatg aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa
    29281 caaaacaatc gttacttcag ttcatgggca aacatcttcc cggatgaaat tggcagtttc
    29341 tacaacttta atggcaattt cccacaaacc gactggtcac cacagagcct ggcgcaggac
    29401 gataccacac atatgaaatc gttatatgct gccactcgtg tcacccttgc cgatccgctg
    29461 catctgatcc tcggcgctcg ttataccaac tggcgggttg atacgctgac ttacagcatg
    29521 gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg
    29581 acctacgcca gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa
    29641 tatctggctc caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat
    29701 agccgtctga ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc
    29761 accggtacac ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca
    29821 gtcagtaaag gtgtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgacg
    29881 tttggcgcaa cgcgctatat tgcagaggat aacgaaggaa acgccgttaa tcctaatctg
    29941 ccacgcacca cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgacg
    30001 gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac gccgtatggc
    30061 accttccgcg ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg
    30121 acgaaaaact tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc
    30181 aacgtggaag gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat
    30241 caattctgaa taacacctgt aaaaaaggca gccatccggc tgccttagtc tccccaacgt
    30301 cttacggatt agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta
    30361 gtaccgaaaa tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc
    30421 ttcaggccag cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata
    30481 catgcgtcga ggttagtaat attttcttta ccaccaaatg cagcaaccag agccggtgcc
    30541 atttcgctgg tacctgtcgc ttttgcatct tcagtcgcat cttcacgacc cggcgttttc
    30601 agatccagtg ctttaatcag cacgcggaag atggtgtagt aaacaattgc ataaccgata
    30661 ccgacgatcg ggaacagcca cagtttgctg ctgttaccag acagaacaat gaagtcgatc
    30721 agaccgtgcg agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc
    30781 aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaaggagaac
    30841 tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg
    30901 cccactttcg cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc
    30961 agaccgtaca ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc
    31021 atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatt
    31081 tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag gcaacgttcg
    31141 atgaaaccgt aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag
    31201 gtctggattg cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg
    31261 gcagccaggc cagaaatgat cggcacaaaa cgtttaccgg caaagaagcc aagatactca
    31321 ggcagcttaa tacggtagaa acggttaaac atgtacgctg cgatcgcacc ggagataatc
    31381 cccccgagta cgccagtatc cgccaggtgt ttagaggcga tttcttcagc aggtaaatgc
    31441 agtaccagtg gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg
    31501 gccagcgcgg atacgccatc gttattggta aagccgaggg cgacaccaat cgcaaaaatc
    31561 agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg
    31621 ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggcagtacg
    31681 gataccggca gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac
    31741 ataattgaga gtgctcctga gtatgggtgc ttttttacgt tctcacgcgt ggcaaggggg
    31801 gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca gagtaaaata
    31861 attcagtgcc gatttgtttg acggctatca cgtttcaact tcagatcatt ggagaagttg
    31921 cacattttga caaaacatat gtcacaatat ttcaaacgtg gttattcacc gcccaatttt
    31981 cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa ctcattcaac
    32041 ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta
    32101 cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct gcaacgtcac
    32161 gaaccatcgc aggttgattc tcttttcctc gatgcggcac cggcgcaagg taaggtgagt
    32221 cagtttctac cagtaaccga tccaggggga cataacgcgc agcatcgcgc agttgctccg
    32281 cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc agtaatttac
    32341 ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt
    32401 cttcgcgcag aattgccagc gtatcggcgc gggcgtcacg ggtatggacg ataaccggct
    32461 tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctgc cgtactttag
    32521 tttccggcgt gtaataataa tccaacccgg tttcacccag cgcaacaacg ccctcttctg
    32581 ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt ggatgcacgc
    32641 cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatgtgt aaataacccg
    32701 gtaatgttgt ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct ttcgccagaa
    32761 cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg cagtgtgagt
    32821 cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac gcccggttgc
    32881 aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt gatgcctgtg
    32941 acggacatta actgttcacg aatgtggcaa acatccccca gtatagcctg caggcgcgag
    33001 ggagaaagat ggtttgccag ttcgacgacc agccccggca catcaacatt ggtcacctgc
    33061 gcagcgccat aatggcgttt tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc
    33121 gccggagctt gttcatgatt aagggccgct agcagcgaat accagtcgcc cgattgcacg
    33181 ctatatgcca gcgcctgaca caatgtttca cgagcctgcc agttatctcc ctgaaacaac
    33241 gccagtgccg cgccaggcga accggcgctt aagcgcaatg cggcaagtag tgcatcctgt
    33301 gacattgtca cttcgcgtga aagccaggtc acggcgtact gttccggcgg cggcgcaagg
    33361 taatgtaacc gacaacgact acgtaatgtt gccagtaagc gttcaggctc gcgggtagcc
    33421 aggaaaaacc aagtttctgc tggtggctct tcaagcgttt tcagcaatgc gttagccgcg
    33481 gcgtcagtca gtaaggcagc atcggttacc cagacaactt tcgcaccacc taagcgtgcg
    33541 tgctcattta acttttcggt gacttcacgt accgcatcaa tgcccagcgc atttttccct
    33601 ttttcgggag ccagggtgta ataatcggga tgcgtgccag cctgcatcaa ctgacatcca
    33661 cgacagtgac cgcaactttt gtggccctgc ggttgttggc agagtaaata acggctcagg
    33721 gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagcag cgcatggtga
    33781 cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa ccatggatac
    33841 catctcatgc gtccaactcc ttcacccagt tggtcacggt agtgcggatt gcatccatca
    33901 cggcctccag cggctgggtg gcatcaatgg tatgaatgct tttatcttgt gctgccagtt
    33961 ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ttcttgctca atacgatcca
    34021 gctcgccgcg cgcacgcgcg cgttttaagc caacctccgg ggtaacatcg agatagagcg
    34081 ttaagtcggg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc atatgttggt
    34141 caataccacg tcctccaccc tgataggctt gagtggagag atcgtggcga tcgccaatca
    34201 cccaggtgcc gttagccagc gcaggtttga tgaccgtttc taccagttga acgcgcgcgg
    34261 cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc gatttgatat
    34321 ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa
    34381 ccatgtcgcg gatacccagt tgctcgagcg tctcaaccac cacgttacgc gcggtagttt
    34441 tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt ttccttaagc
    34501 actttcagat aatcctgcac agacttgtta tgactggcaa gattggtatt aaacgtgtga
    34561 ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg cgcagcagcc
    34621 ttcagcgaat ccgcccccgg cgtcgctatc gcacctggcg gcaaaccggt aatggtatag
    34681 gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt ataacgctct
    34741 cccatcccgt aaatcacggt cgggtcggtc tgcaggcgca taccaatgcg taaacggttg
    34801 ataaataccg aggcaacctg atcgcgttcg ctggaaacgg cggtttcttt ttcgataatt
    34861 gatgccatcg tcaccagctg gtttttatct ttatagggca gaccgtccgc acgcccttcc
    34921 caggcgctat cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa cgcgacatcg
    34981 gtggtattgg cggtatacat ccaggtgtct ggccagaacc aaccttcaat ccactccggg
    35041 ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcgctcag cgtgtgcttg
    35101 atatacgggg cctcacgcaa ttgcttgagg taatcgctca gacgcatccc ttctaccagt
    35161 cgcagaggga actgtgcttc tttaccgctt tccagcaatt tcagcatctc gcgcacggtc
    35221 atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg ttcgatacgc
    35281 agcagccatt ggaaaacccg tgggcgattg atgatcttat cggcataaag ctgttcaccg
    35341 agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt gataagcaat
    35401 ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat acccagtacc
    35461 accagcaata acaagataat caataacact tttttcatga ctaattcggg cgctcacaaa
    35521 gtggggctaa atattcatat aacgttgctg acgaaaagga gacatcgcca caggcacgta
    35581 cgggtatcac tggcattaac gcattacaaa taaccatctc atctgcctgc aacgcctcct
    35641 ccagcgaggc ttgcacttcg acaagctgat aagaggattg tgccagtaaa cggatacaga
    35701 attgtcgcat aatgccgtta acacctgcct gatccagtcg cggcgtataa actacgttgc
    35761 ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg ctgtcaagga
    35821 ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg gatcaatact tgctcaagcc
    35881 gattaagatg tttaatacct gcaagatgag gattgcgccc cagccgcacc gggcttagcg
    35941 ccaacgtcat cccctcgtta cgcaaacggt cgtaatgtgc gggataaacc gtaacggaga
    36001 gaatccgcgt tgccggtccg ctgttcaata tgctgtatcc tcgcccgcca ctaccgcgac
    36061 tgatcaccac tttcagcaca ccattttgct gttctgctgc cagcgttttc atctctagtt
    36121 caagctgagg ccagaagtca caggaaatca tcaaccgctg acaagcatcc tgtagtcgct
    36181 ggatatgcgc cgataacaaa ctgactttgc cgtcgataac tctggcggtg gtaaaacaac
    36241 catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta tgaccgttaa
    36301 ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgt tcactacgga
    36361 acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat aagggtggaa
    36421 aatgacaatt tagatctttt taaagatcaa agaaccatta gtgccaccga agccgaagga
    36481 gttacacagc gtgtattcca ttccgctaac ctgacgcgct tcgtgcggta cgaaatccag
    36541 atcgcaacct tcatccgggt tatccaggtt gatggttggc ggaacagcct gatcgcgcag
    36601 cgccaggatg gagtagatag attctactgc acccgccgca cctaacaggt gaccggtcat
    36661 agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg ttttcaccgc
    36721 ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat
    36781 ctgactcgct tcaatgcctg catcacgcag agcatttgcc atcgccagag ctgcgcctgc
    36841 gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa agccgacgag
    36901 ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa gtactagcat
    36961 accggcacca tcgcccagta cgaaaccatc acgctcttta tcccacgggc ggctcgccgc
    37021 ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac caacgcccag
    37081 cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc catacgcgat
    37141 aatacgcgca gcatggccaa tgttgtgcac gccggaagta caggcggtcg cgatagagat
    37201 gctcgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt tcacaatcgt
    37261 tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag atgtgtggtt
    37321 ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc
    37381 gttctcttcc gttatttcaa ggccagaatc ctgcatggcc tgaacgccag cgacaattcc
    37441 atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt cctcacagtt
    37501 aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga aatggtcgat
    37561 taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta cggtattgcc
    37621 gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca cgtttgtcct
    37681 ccagggaggg aaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga
    37741 ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac
    37801 ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc
    37861 cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt
    37921 gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc
    37981 ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt
    38041 agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac
    38101 gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata
    38161 accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat
    38221 cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa
    38281 tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag
    38341 gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg
    38401 agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa
    38461 gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc
    38521 aaccacagaa ccgatagtga taatgcgacc atgacgcttt ttcatcatag cgcgcattac
    38581 cgcttttgac agacggaaaa cagatgaaag gttagtttcg ataatatcgt tccactcttc
    38641 atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc
    38701 cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt
    38761 cacattcaac atcagacctt tgccgttggc acctaaataa tcactgatcg cctgagcgcc
    38821 attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat
    38881 tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat
    38941 gattttcctc ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga
    39001 ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagta agcactttgc ccgggccgac
    39061 ttcatagaga tgttctacgc cttgcgctgc catgtattca acagacttcg tccactgaac
    39121 cgggttatac aactgacgta ccagtgcgtc acggatggca tcaccattgg tttcgcattt
    39181 cacatcaacg ttattcacaa caggaactgt tggtgcgtta aaggtgattt tcgctaattc
    39241 tactgccagt ttgtcagctg ctggtttcat cagcgcacag tgagacggta cgctcactgg
    39301 taacggcaga gcgcgttttg cgcccgcagc tttacaggca gcgccagcac gctcaactgc
    39361 ttctttatga ccagcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac
    39421 ctgaccttct gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat
    39481 tgccgccata gcgcccgttc cttccggtac ggcttcttgc ataaacttac cgcgcatctc
    39541 aaccagacgc accgcatcag cgaaatcaat cacgccagcg cataccagtg cggagtattc
    39601 ccccaggctg tgaccggcca tcattgccgg tgctttacca ccctgctgct gccatacgcg
    39661 atacagcgca acagatgcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc
    39721 ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc
    39781 agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt
    39841 ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta
    39901 gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa gcagaaccaa
    39961 ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc
    40021 cgcagaggta ttaccgtggc gatccagcgt caccacgaca ttgtccatcg acatgccgag
    40081 ttttttcgct gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag
    40141 ttgagaacgg tcaagattat tcgccgtcag cgtctcatca acgatgtgcg ccagttctgt
    40201 taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt
    40261 cacacggtca gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg catgcagatg
    40321 ggtggagatg attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc
    40381 aaaaataata atagtcccac gatctgttgg atcgcaggtg cgcgccagta catcggaacc
    40441 gacgaccaga gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc
    40501 ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccag
    40561 catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac
    40621 aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc
    40681 ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc
    40741 ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt
    40801 ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca
    40861 gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct
    40921 accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt
    40981 ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa
    41041 tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca
    41101 atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca
    41161 ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac
    41221 cctgagattt cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaatgtga
    41281 catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg
    41341 cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc
    41401 gaatactgtc gagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat
    41461 tgggaatttc caccacctct tcagccagaa ctgagcccat aatggcaaat tgcaccaaca
    41521 ttgtactgtc acaatcgacg ttggccccta aatcgaggac caccgttttg cccttttgct
    41581 gatgcggtaa taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata
    41641 attttgccag ccccatcagc gccccggtat taccggcact gacacaggct tgcgctcgac
    41701 cttctttcac cagctccagg gccacgcgca ttgaactccc acgactggcg cggatagctt
    41761 gcgaaggccg ggcatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac
    41821 gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa
    41881 gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag
    41941 ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc
    42001 cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca
    42061 gacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg
    42121 gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag
    42181 cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc
    42241 catggacctt actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt
    42301 acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcgcttctt caggcagttc
    42361 accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac
    42421 cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc
    42481 gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac
    42541 aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca
    42601 ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat
    42661 agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg
    42721 ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac
    42781 cggatcgaga gtcaggggta attttacctt ttgcataggg cgcgcatatt aactttgtaa
    42841 cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca
    42901 ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga
    42961 agcgcatagt ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct
    43021 aaacttattt tagcctccac ttcgccctgg cgtcgcgctc ttctggaaaa actgcaaatc
    43081 tcttttgaat gtgcagcacc agaggtcgac gaaaccccac gcagcgacga atcaccgcga
    43141 cagttggtgc ttcgactggc acaagaaaaa gcacaatctc tggcgtcacg ttatccggat
    43201 catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg
    43261 ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc
    43321 tatactggac tggcgttgtt taattcggcg aatgggcatc tacaaacaga agtggagcct
    43381 tttgacgtcc atttccgtca cctgagcgag gcggagattg ataattacgt gcgtaaagag
    43441 catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac tctgtttgag
    43501 cgtttagagg ggcgtgatcc taacacgctg gttggtttac cgctaatcgc gctgtgtcag
    43561 atgttacgtc gggaagggaa aaacccgctg atgggatagt tgtttgcgcc ggatgcggtt
    43621 acgtcgcatc cggcaatcgg tgctggcggc ctgatgcggc gtaaacgcct tatcaggcca
    43681 accgcccatt atcatgctta tatgctcgta ttagcgcgcg ttacgcagct tttgcaaaca
    43741 acgcttcaaa ccatcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg
    43801 ggtaaacttc aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc cggcttcagt
    43861 gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc
    43921 atactgtgta tgcacacgga tctgatgagt acgccctgtt accgggctac aacgcaccag
    43981 ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg
    44041 actgacacgc acgatacgtt cgccgctttg cagaatattt ttcagtaacg gcgcttgaac
    44101 gctcttcaca tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcatccc
    44161 tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa
    44221 cacacctgag gtgtcccggt caagacgatg aaccagttca aggaaccgcg cttccgggcg
    44281 caacgcccgc aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt
    44341 accggaaggt ttattcagca ccaggatatg gtcatcttca tataagatga cgtccgccag
    44401 cgctgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac
    44461 cggtgggata cgtacttcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt
    44521 gttcacccgc acttcgcctt tacgcaaaat acggtaaatc atactttttg gtacgccttt
    44581 caattgggta cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac
    44641 aatttttacg gatggagtct ctgttttcat ggtgggcgat tctaattagc caacaggatt
    44701 cgcgccactc atttttctat gcttatattt agttttgcac cttattactt cactacgtga
    44761 tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag
    44821 cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc
    44881 cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga
    44941 ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg
    45001 cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt
    45061 ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt
    45121 tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac
    45181 ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta
    45241 cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag
    45301 atgggcagcg tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa
    45361 acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt
    45421 acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg
    45481 ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag
    45541 tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta
    45601 tcagtctggc gggtagctat ctggttctga tgccgaacaa cccgcgcgcg ggtggcattt
    45661 ctcgtcgtat cgaaggcgac gaccgtaccg aattaaaaga agcactggca agccttgaac
    45721 tgccggaagg catggggctt atcgtgcgca ccgctggcgt cggcaaatct gctgaggcgc
    45781 tgcaatggga tttaagcttc cgtctgaaac actgggaagc catcaaaaaa gccgctgaaa
    45841 gccgcccagc tccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg
    45901 attacttacg tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg
    45961 cacgccagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca
    46021 ccggcgagat cccgctgttc agccactacc agatcgagtc acagattgag tccgccttcc
    46081 agcgtgaagt tcgtctgcca tccggtggtt ccattgttat cgacagcacc gaagcgttaa
    46141 ccgccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaaccgcgt
    46201 ttaacaccaa cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg
    46261 gcggcctgat tgttatcgac ttcatcgaca tgacgccagt acgccaccag cgtgcggtag
    46321 aaaaccgtct gcgtgaagcg gtgcgtcagg accgggcgcg tattcaaatc agccatattt
    46381 ctcgctttgg cctgctggaa atgtcccgtc agcgtctgag cccatcactg ggtgaatcca
    46441 gccatcacgt ctgcccgcgc tgctccggta ccggtaccgt gcgtgacaac gaatcgttgt
    46501 cgctctctat tctgcgtctg atcgaagaag aagcgctgaa agagaacact caggaagttc
    46561 acgccattgt tcctgtgcca atcgcgtctt acctgctgaa tgaaaaacgt tctgcagtga
    46621 atgcgattga aactcgtcag gacggcgtgc gctgtgtgat tgtgccaaac gatcagatgg
    46681 aaaccccgca ctaccatgtg ctgcgcgtgc gtaaagggga agaaactcca accttaagct
    46741 acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg
    46801 aacgtaagcg tcctgaacaa cctgcgctgg caacctttgc catgccggat gtgccgccag
    46861 ctccaacccc agctgaacct gccgcgcctg ttgtagcccc agcacctaaa gctgcaacgg
    46921 caacaccagc atctcctgca caaccagggt tgttgagccg cttcttcggc gcactgaaag
    46981 cgctgttcag cggtggtgaa gaaaccaaac cggccgagca accagcaccg aaagcagaag
    47041 cgaaaccgga acgtcaacag gatcgtcgca agcctcgtca gaacaaccgc cgtgaccgta
    47101 atgagcgccg cgatacccgt agtgaacgta cagaaggcag cgataatcgc gaagaaaacc
    47161 gtcgtaatcg tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcagg
    47221 ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta
    47281 gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcaaaggcg ctgaatgttg
    47341 aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc
    47401 gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg
    47461 tagtcgcacc agtggttgaa gaaactgtcg ctgccgaacc aattgttcag gaagcgccag
    47521 ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac
    47581 agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc
    47641 gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt
    47701 atccaaccca atcgccaatg ccgttgaccg tagcgtgcgc atctccggaa ctggcctctg
    47761 gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaggtc gaagagcagc
    47821 gcgaacagga agaagtacaa gtgcagccga tggtgactga ggtccctgtc gccgccgctg
    47881 tcgaacctgc tgttagcgca ccggttgttg aagaagtggc tgaagttgta gaagcccccg
    47941 ttcaggttgc cgaaccgcaa cctgaagtgg ttgaaacgac gcatcctgaa gtgattgccg
    48001 ccgcggtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag
    48061 ttgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag
    48121 ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc
    48181 cagtcgtcgc cgaagtcgca gcagaagttg aaacggtagc cgcggttaaa cctgaggtca
    48241 ccgttgagca taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc
    48301 cggaggcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg
    48361 ctgcaggtgg tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg
    48421 ttgagtaata attagctcaa gtaatcaagc cctggtaact gccagggctt ttttatttca
    48481 gggtagcatc tttgttgtaa tgacattagc gttacatgcg ctgtataatt atcatccatg
    48541 caaagccgaa ggatatattt atgccagcag ccaataacat tccaaccagg cgcgaatctt
    48601 taaatttgcg cataaaaccc gcagaacgcg atcttattga ccgggccgct aaagcccgag
    48661 gaaaaaatcg caccgaattt gtccttgagg ccgccagagc cgcagctgaa gaggcgctca
    48721 ttgagcagag aattttcagg atcgattctg atgattacca gctatttttg aatcgtctgg
    48781 atcagccccc cgcgcccaat gctgcattac gcaaaaccat gcaaacaccc gcgccgtggg
    48841 agcaaaaaaa atgattcagg cccccgagcc actcatggca aggcatcaat tcacctcttt
    48901 ttgctcggga gtagaaacga tggataactg gctaaagcag agagccctaa aaaatcagct
    48961 tgccggcgca tcacgtacat ttgttagctg tgatacgtat agcaacgttt tggcatatta
    49021 ttcgcttgct tccagcgccg ttgaaacgta tgtcgcgaca ggacgatttc gccgaaatat
    49081 gccagaaccg atccctgttg tagtacttgg tcgtctggca atagataaat cattacaggg
    49141 tcagggcatc ggtcgtgcga tggtcagaga tgccggtcta cgagtactac aggcggctga
    49201 agtcattggt attcgcggaa tgttggtcca tgcgttatca gaccaggccc gtgagtttta
    49261 tctgcgagtg gggtttgaac catcgcctgt cgattctatg atactgatgg caacattggc
    49321 tgatttgcag gaatgtctta aatagttcgt tatgtctcat gcaaaaaaat gcccgccccg
    49381 gcgatcccag agcaggcaga gaaaacatac ccagtttcat gatatgtttt aaagcgagct
    49441 tatttgttga gctggaacag cgacaatccc tgcatatcgg taaatgcttt atacgatgcc
    49501 tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc cacatcaacc
    49561 agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc tacctaatga atccagcgac
    49621 tccagttcgt tcagctgcgt gcctaattcc gcgcgaacag tcagcacatt gttcagcgag
    49681 tttttcagtc cgcggttggt tttatccagt gccgcagcgg cggtttcttt atccgcttcg
    49741 ctatccgcga ccggcgtttt tagggctgcg atggcgctat ccagcatggc aaaaagattg
    49801 gtttcagaag cgctaccgtc tggttccgct accgcgttgc tggtaatact gtcgaaaatt
    49861 ttgtcacccg tgtgccctat caccatcgaa cgcgaagcat cgacctgttg tttaatactt
    49921 tctgctccgc cttcgtattt gccatccgcc tcactaaacg gcgcagtttc tgttttataa
    49981 ccggcaaaaa tgtagcgccc gttaccgtca gtggtgttcg ccagattcag caactggtca
    50041 cgaagcccct gaatatccgt agccagcgag gcacggtcat cgtcactcaa ggtgccattg
    50101 ctggcgtaga caattttttc ctgagcattc tggatagcgg tggtgacctg gctaagtaca
    50161 ctctcttcca gtgacacttt ttgagtggcg aaagtacgcg ccagcgtgta ctggctgttt
    50221 tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc atctgaaggg
    50281 ttaacgactc gcttacccgt cgacatctgt tcgccgtact tcatccattc tgcctgagaa
    50341 ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct
    50401 ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg
    50461 cattcgcgag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga
    50521 caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct tgagtggtgc
    50581 tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt
    50641 cgttaaagga tttcgcaccg cccaccgttt tactgttgct ttgcagatcg agcagggcct
    50701 gaccgttgcg gttgtcgcta tcacccgcat cttctgcgct cgccatggcg attttcgctt
    50761 cgtcggtgat taatacatcc atgttgacga tggcgtcact taccggtttc agcgtgaagc
    50821 tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac
    50881 cgttggcatc cggcgtcacc gtaaaagtgg tattgctggc aaggcgggtg acctgccact
    50941 gattattatc gaacgagatt ttgtaatctg tcgccagtac cgcggaggca tcagttaccg
    51001 ttgcaccgat cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga
    51061 tagcaaagaa atcttcaccg gcatcgccgt tagcatcaaa tccggctttg tgttgggtgt
    51121 tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtcaagat
    51181 cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga
    51241 tctcaatatt gcctgccgtc ccatcaacat aagcgacagt cgtacgagaa gggtcggcgc
    51301 tggaaggaac tgccgcaagt tgccgcgccg tacttccctg aaccagtgag tagccattgg
    51361 ccatcgtgat gttataagtg ccaccatcct gaacgctgac ttctacgcca acaatctggt
    51421 ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc
    51481 ccacgcctgt caggcgggag atctgatcgt tcaggctggc gatttgttta gcgtaattgt
    51541 tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat
    51601 attgatcggt ggttttaaac tgattcacca atccttctga tttcccaatc agcgcctggc
    51661 gcgctgccgg atcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca
    51721 tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct
    51781 catagcgggc ggtcaggccg ctactttgcg tctgcgcagc acgtaactgg ttggtaataa
    51841 acgcatcata ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag
    51901 cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt
    51961 tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc
    52021 tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca attacagagt
    52081 attatcggca gcgccccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct
    52141 gtaggttttg ctcaccttgt cgcttatcga tttcatctgc tgaatcatgt tggtgagttt
    52201 gcgggcatag tgaggatcgg tggcatagcc cgcgtcctgt agggcctgcg ccccctgctc
    52261 cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgcgttaaca gcccaacgta
    52321 atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc
    52381 ttcgccattt tcatattcag tcgtggtgat ttcagtgact ggccctttcc agttgccaga
    52441 ggctttgacg ccaaacaggt tatagctcgg ctcgccgttt tcacggcgga tttgccgttg
    52501 tccccagcca gattccagcg ccgcctgagc gaggatcaaa tgatgtggca caccgctttg
    52561 ctggcttgcc agttgggcgg gcaacgagag ttgcgcgagg aatgctttac tgtcacccgg
    52621 cagcgaatca tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctgatt
    52681 ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctgccg tgggctcctc
    52741 tggcaatggt tgttctggcg tcatctgttt aaccatcatc tctgccagcc ccagaccttt
    52801 gcccgccgtc atctgttggg caatctgctg gtcatacata ctggtataca ggcgagtgtg
    52861 ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg
    52921 cacgaacatc ccttccacct ggcgggccac cggacggata tttgccgccg gatcttcgcc
    52981 cgctttcgcc ttaagttcgt tgagcgattg tgcatcccag gccgcacttg ccagtagttt
    53041 gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt
    53101 gattgcagta tggacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc
    53161 acgttattga ggctggcgct agaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa
    53221 tcgatctgcg tttgtggagt aaccacagtc tgcccaccac caaacggtgt atctggctgg
    53281 ctgacattgg cctgacgatt aactgttact gagagattcc cctgcgctac cgcgcagctg
    53341 tcgagggtca cttcgcgatt catcaccacc gaaccagtgc gcgagttaat cactacttta
    53401 gcgtcctgcg gggtaacatt aacctgcata ttctggatat cggcaaggaa acggacctgg
    53461 gaactgttgc cactcggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg
    53521 ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatact gaaatcttcg
    53581 tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca
    53641 ataaccgcac cattggtgat ccgtccaccg ttcagttggt tcacctgaac actgctaccg
    53701 ccagcggagg cccctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga
    53761 ctgtcaacgc ccttaagcgg tgtcatcaat aacgtaccgc cacgcagact tttggcattt
    53821 cccatggaag aaaccaccac gtcaatggtt tgcccctgac gtccaaacgg tggaagtgac
    53881 gccgtcacca ttaccgcagc gacgtttttt agctgcatat tggtgcccgt cggaacggta
    53941 attcccagtt gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgagttgtc
    54001 tggtcacctg taccatcaag ccccaccacc aggccatagc caatcagtga gttttgcctt
    54061 accccctgaa cactggtgag atcgcgaata cgctcagcct gaaccgccgt cgtaaccagt
    54121 agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt ggcgacagat
    54181 taaggaagaa acgttgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta
    54241 cgtattcaat gcgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag
    54301 tgcgtggatt aactacacca gagaagcgaa taaattcggt accctgatta atggcgatct
    54361 gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca
    54421 acgtgccgct aaaggtattg ctggcattgg ccccgccttt tccgttgaac gtgttaccac
    54481 cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag
    54541 tatcaaagcc aaaattagat ttaccgtcac ggctggcatt cgcagaggag cttttgctgg
    54601 cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat
    54661 cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt
    54721 tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgt accagcggcg
    54781 tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag
    54841 tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaatctg
    54901 ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggatacc
    54961 gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc
    55021 acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc
    55081 ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaata
    55141 ctctccagtc cggtatcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga
    55201 gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac
    55261 gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc
    55321 ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgagcc atctggcaac
    55381 atcacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttatt ggtttgcgac
    55441 aggttgccct ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt accgatttgt
    55501 aatccggatg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga
    55561 taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttggtact aacgtttgcc
    55621 aggttgttgg caatgacgtc catattggtt tgctgggcat ccaggcccgt tttggcgatc
    55681 cataatgaac tgatcataaa atgtcctgta gtggattagt gacaagcaga gcagaagcac
    55741 gcgacggtgc gaatttcccc tcaccctaac ctctcccaaa atgggcgaga gaatagtccg
    55801 cgcgctttta ttgcaatgtc atcaatcgtt taattaactc atcgacaaca gttggttggc
    55861 acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc
    55921 gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaactcc
    55981 cgacatcaca cgcaaggttg gatctgcctg cagtaccggc ccacgcgtgg cctggctttc
    56041 cgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac
    56101 cagtttcaga cgccccactg gcgcaaccgt atttgccgga tcgcccggat tcagcgccga
    56161 gattgtgcca tcggcagcaa tagtgatttc cgccccttcc ggcacagcaa ttggccccgc
    56221 ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct
    56281 gccattacgc gtataccctt cgctgccgtc agcggtctgc acggccagcc agccatcctg
    56341 ctgcaacgca acgtccagcg ggcgcgaggt gtaatccatc ttgccgggcg tcatgtctgc
    56401 acccggcgtt gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac
    56461 tgcgcgcaag gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg ccagattact
    56521 ggcggttacc gcctgttgat tcaacgtctg gctggctgct cccatcgcgg tataaattgc
    56581 gtgatccatt gagctatccc gtcagcgatt agcgtaagtt aaccagcgtg ttgaggatct
    56641 ggtcctgggt tttgatggtc tgggcgttag actgatagtt acgctgggca acgatcatat
    56701 tgaccagttc tttactgaga tcgacgttgg acgcttccag cgcaccgttg gtcagggtgc
    56761 caaagttgcc cgtcccggct gtccccaaca gcgccacgcc agaagattgc gtcgcagacc
    56821 agacgttgtc gccttcggat gccagacctt cgttgttggc aaagttcgcc agtacaatct
    56881 gccccagcag ttgggtttgt tcgttggaat agttgccgac aaccgtaccg tcatcattga
    56941 tttgataact caccagatcg cccggtttgt agccgttctg ggtggttgcc acaatgttgt
    57001 tagcaccggt attttgctgc atggagttga ggaaactcag gctaaacgtg gcgggatctg
    57061 cgccgttaat tgcgccagtg gaaatattcg ctgttggatc tgaggtcaga acgccattgg
    57121 cattaaacac caatgttgtc gctgtcttcg caatgctgtt tggatcacta ctatcctggg
    57181 tgtagacctg ccagttatta tccccggtct tcacaaagta gacgctcatg tcatgagcat
    57241 taccctgact atcgaaaaca gtcaccgaac cttttttgtt atagctatcc gcattgctgg
    57301 catcaaatgc gttaacagag ggaagcggat cactggaatt caggttgatc tgcatcgacg
    57361 ccgtggtggt agttttcgct gccatcaggg tattcggaat cgaaatattg gtcggattcg
    57421 ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta acccgtcagc tgtaaacctt
    57481 gcatattcac caggttacgg ttttcatcca gcttaaattg tccgttacgg ctgtagaaca
    57541 ccgaaccgtt gctgtccacc agacggaaaa aaccgttctg gctgatagca acgtccagac
    57601 ctcgcccggt gttggtggtc gtgccatcgg taaagtcctg agtgataccg gcaactttta
    57661 cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa
    57721 agccgtaggt ggcggagttg gcgatattgt tgccaataac atcgaggttg gtggcggcag
    57781 cgtttaatcc gctaaccgct tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt
    57841 aaattatctg ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga tccagcgtat
    57901 taccgttgtt gccgcggatc acaccctgca ccagagcaaa ctgcagcggc tgggcaacca
    57961 gttgtgtacc accgttactg gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc
    58021 tgccgtcagt caacgtaccg tcccaggtga aactgtggac tccggcagtc agttcaccga
    58081 tatcaatggt acggacaacc gcaccgtttt tatcggtgat ggtggccgta actttgtctg
    58141 ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt cacagcccct tcttcactgc
    58201 ctgttcccgc aagaacagtg gtgccgggga tcatcacgcc gtgaccgatc aggttactgg
    58261 cctgtaacga ctggctgtta tcaatctgtc cggaaataga tccgagcgtg gtattgagtt
    58321 tttcaatccc gctgaccgtg ctgatttgtg ccagttgcga cgtcagctcg ttgttttcca
    58381 ttggattggt cgggtcctgg tttttcagct gcgccaccaa caaagtcaga aaactgcttt
    58441 gtaaatctgc ggcgttgctg cccgtgagcg aactactact ggtggtgctg acgccggtat
    58501 ttgtcggatc ggtggtggtt accgcaatgg acatagcttt ctcctttatt gaccgagcgt
    58561 aagggttttc agcatcatgc ttttcaccgt gttgagcact tcaacattgg cctgatagct
    58621 gcgtgacgcc gacatggtgt taaccatctc cccgacgaca tcaacgtttg gcattttcac
    58681 gtagcccttt gcatccgcca gcggattacc cggttcataa accagtttgt ccggggcctg
    58741 actttctata acatcggcaa cttttacgcc gcctgtcgca gcacctggcg cagcgttaac
    58801 ctgaaatacc acctgttttg cccgatatgg ctgtccatcg ggaccggtca cgctatcagc
    58861 attcgccaga ttactggccg ccacgttcag gcgctgggac tgagcagtta acgccgaccc
    58921 ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat
    58981 catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc
    59041 ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg aaggctggtc
    59101 cggaatacgg tattgcagtt ctgcggtagg aggtgtcagc gcctgcgccg gaatgtgttg
    59161 cgttgaggtc atcgtcagtg caaccacgct tgttgcatcc cgtccacgtt gcatgacttt
    59221 tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat cggcattggc
    59281 gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa
    59341 acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa aattctgcca
    59401 tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat gggtcgctat
    59461 ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagatat ggctgggggg
    59521 cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa
    59581 gtgcggcgag caatctcaca tcgcaattgc acaccttttt tagcgcccag ctcgcggggg
    59641 taagtgatga ggttcgtgtt tctattcgta cagcgcccaa tctactaccg ccgtgcgagc
    59701 agccattgct ttcgatgagc aataattccc gcctgtgggg caatgtgaat gtgttggcac
    59761 gctgcggtaa cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg
    59821 ttgccgcaat gcccattgtg cggggaggaa agctggaagc tggcaatgtc aaactgaaac
    59881 gcggacggct ggacaccttg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg
    59941 ccgttagcct gcgcgatcta tcacccgatc aacctatcca gttaacccag tttcgccagg
    60001 catggcgggt aaaagcggga caacgcgtca acgtgatcgc cagtggtgat gggtttagcg
    60061 ccaacgcaga aggtcaggcg ctgaacaatg cagccgtcgc acagaatgcg cgggtgcgca
    60121 tggtatcggg acaggtggtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc
    60181 tgtaagctgt taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg
    60241 ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag cccgtaagca
    60301 ccgttcaacc gcgcgaaaac actgacgcgc cggtaacgaa cacccgggcg gcaaaaacaa
    60361 ccgcctccac cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg
    60421 gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg
    60481 aactaaaaat ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact
    60541 tgcagagtaa ctgatcgtat gacgcgtctt gcagagatcc tcgaccagat gtccgctgtg
    60601 cttaacgatc tcaaaacggt aatggatcaa gagcagcaac atctctctat ggggcagatc
    60661 aacggcagcc agttgcaatg gattacagaa caaaaaagct ctctgctggc gacgctggat
    60721 tacctcgaac agttacgcag gaaagaaccc aatgcagcaa atagcgttga tattagtcaa
    60781 cgctggcagg aaattactgt gaaaacgcag caactacgcc aaatgaatca acataacggc
    60841 tggttactgg aaggacagat tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat
    60901 caggaaccga cgctatatgg agcgaacggt cagacctcaa caacccatcg cggcagtaaa
    60961 aagatttcga tctgaagatt atctccggcc tgcactgcag gccggaatac attgttacac
    61021 cgtccggcgg gcaaactctt taactttgaa gcccagtacc gccagtgcag caaagtacgc
    61081 agcaatcccc gccagcacga ccgccattaa acgcagtaag cgccagggca tggtacccaa
    61141 tgaccactcc ggcatgatat gtaacatacc taaaagcacg cccgacatca ccagtaccgc
    61201 caccaccaga cgcaacagaa acgccatcca gccaggttgc ggggtaaaga ttttctgctt
    61261 acgcaactgc cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga
    61321 cagcccggca tgtttcaacg gaccgataaa cgcaaggttc atcaattgcg ttaaaatcag
    61381 cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg cgggaataaa agccaggagc
    61441 caacactttc actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg
    61501 ggtcatcagc gcatcaaagg cggtaaattt accgtactgg aacagcgaaa cggttaacgg
    61561 acctgaaaga atgcccaacg caaccgcact cggcagcgcc aacaggaaac aaagacgcag
    61621 cccccagtcc atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag
    61681 cgacggcagc aaaatcgtgc caagcgccac gcccagtaca ccggacggaa actccattaa
    61741 gcggtcggcg taatacatcc aagacaccga accggaagca agaaacgagg caaaaatggt
    61801 gttgatgatt aaggagatct gactcacaga gacgccaagg atcgccggtc ccatctgttt
    61861 caccacgcgc attgctccgg catcgtggaa gttaatgcgc ggcaggacca gcatgccgat
    61921 cttcttcagg tgcggtagct gatacaccag ctgcaggacg ccgccgaccg taacagccca
    61981 cgccagcgcc agcaccggtg ggttaaagta cggtgcggca aacagcgcga aaccaatcat
    62041 gctgatgtta agcagtgttg gagcaaacgc cggaatcgag aagcggttcc acgtattcag
    62101 aatcgctccc accagcgacg ccagggagat cagcaagata tagggaaagg taatctttag
    62161 tagctggctg gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat
    62221 gatcacccaa ggtgcagcga gcatgcctgc gaccgtcaca accgccagcg cgagtgtcaa
    62281 cagaccagaa acatacgaga caaagacccg cgtggcgtct tcaccctgct tgcttttgta
    62341 ttccgccaga atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa
    62401 caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat
    62461 tctggcgaca attgcgtcac gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct
    62521 gacggcggcc agcgatttta ataaattcat cggtgttcta atccagaccc acgggcaaaa
    62581 cgcctgcaag gcaggcgctg aaagaggcga atagtctacc tggattatgg tgaattgcta
    62641 ccgccagatg ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac
    62701 gttgcgccag cactgcctgt tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt
    62761 caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc aagcgtactc tgccaaccag
    62821 gaatcggttt attcactaca ccctgcccac gctcctcacg ccattcgcgc atatccgtaa
    62881 tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc acgctggctt ccggcacggc
    62941 gatgcataca ggtggtgatc tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca
    63001 tttcgccagc gtcattagtc agtagcgtac cgccatccag agaggcttta ccgcccgaca
    63061 accacagcgc ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt
    63121 gtggcccgac gctattgcta cgatgtttat ccattcttag cgaggctgcg gtggcgagtt
    63181 gcttttttaa ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca
    63241 gttttttccg cgccgccagt tccaccagcc gttcagcatc gcgcagattt tctgccagcg
    63301 gtttatcgac acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtggc
    63361 tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc ggcaaggctg gataacgaat
    63421 cggcataagg aatgcgccag ctgtcacaaa ttggcagggc tttcgcgcgc gtaggcgacc
    63481 acgcaccttg taacgtccag tcagacgctg ccgccagcac cggtaaccac gccttttgcg
    63541 cgatgccgcc taaccccact acgccgatac gtaatttttt cactttaatc tcccagatgg
    63601 gccagcaacg aatcaagacg ctgtttcagt tctgccactt cgatttccag agcttcgacg
    63661 cgggcctgta aatcaccgtc aaccgcgttc gacatagccg ttaccaccgg ctgatcttca
    63721 acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga
    63781 cgcaccacaa aaggaccatc ttcgcgattc gccagttgtt ccagcgtcga ctcaacttcc
    63841 gccatatcgc tgaattcata cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg
    63901 gcaccacgca ataacaacgt ggtgattaac gccacttctg cggcgctcag tttcagatcg
    63961 ccaaattctg aattacaaaa acgttgctca tatttggtga cccgattacc aaaaccgctc
    64021 accgtgcgta gataatgacg tttgaccaga ttatccagct gttcctgcac ttcagattca
    64081 ctcagattca tgaccggttc acggttcgtt ttctgattac aggccgtgac tacaccattg
    64141 actgagagcg ggtattgctc cggcgtggtc acctgctttt ccagcaagca accaatcacg
    64201 cgagcttcaa gggcagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca
    64261 gtctggggta gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata
    64321 gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt
    64381 attgtgcggc atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg
    64441 aatggctgcg gtcagggctt caaacatgag tcctttgccc tgccattttt gcccaatcga
    64501 ataaccgaga tagcaggcat gaaaagagcc acgaacaaca ttggaaaaat tggcaacgcc
    64561 aataatctct ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac cttgtttatg
    64621 aaattcgtta atcatcccta gcctggcctg ccagcctgat ggataacagt ggctttcgtc
    64681 gcgcactggc tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgcaagacg
    64741 ccaggcatca cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt
    64801 tggcacgtta ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat
    64861 acgctgtaag tattactata cctgcgcaac cagaccctgt gaaaagtcca gaatgccatt
    64921 ttctgcatta ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa
    64981 atattgtccc cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat
    65041 cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag gccaggaacc tgggtaaata
    65101 tttcctgctc atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat
    65161 ctctatccgc ttcgttgatc aaatgggctg ggccgccgta atggtcggta ttgccctcgg
    65221 cctacgccag tttattcagc aaggtctggg tattttcggc ggagcaattg ccgaccgctt
    65281 tggtgccaaa ccgatgattg ttaccggtat gctgatgcgt gccgccggat tcgccacaat
    65341 gggtatcgcc cacgaaccgt ggctactgtg gttttcatgc ctgctctcgg gactcggcgg
    65401 cacgttgttt gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc cacaacagcg
    65461 tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca ttggcgcatt
    65521 gttggggagc tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggcagtttt
    65581 gtttgtgctg tgtgcagcgt tcaatgcgtg gttgttaccg gcatggaaac tctccactgt
    65641 acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta
    65701 tgtcctgacg ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat
    65761 tatggtcaac gacgtggcgg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc
    65821 gtgtctgtcg ttaacgttgc tctatcctat cgcccgctgg agtgaaaagc attttcgtct
    65881 ggaacatcgg ttgatggctg ggctgttgat aatgtcatta agcatgatgc cagtgggcat
    65941 ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg ttttatatcg ggtcgatcat
    66001 tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggca gacgcaagag ctcgcggcag
    66061 ctatatggga tttagccgtc tgggtctggc gattggcggc gctattggtt atatcggtgg
    66121 cggctggctg tttgacctgg gcaaatcggc gcaccagcca gagcttccgt ggatgatgct
    66181 gggcattatt ggcatcttca ctttccttgc gctgggttgg cagtttagtc agaaacgcgc
    66241 cgcgcgtcgt ttgctggaac gcgacgcctg atttgatact cccccctgac tcctccatac
    66301 tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg
    66361 cccttactgc ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca
    66421 tgttctgctc aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc
    66481 cattctgcaa aaagatgaca gccgctatat gccagaaagc atggacatcg ttcactatgt
    66541 cgataaactc gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg
    66601 gctgcgcaag gtcaatggct acgccaacaa actgctgttg ccgcgttttg ccaaatcggc
    66661 atttgatgag ttttctactc ctgccgcgcg caaatatttc gtcgacaaga aagaggccag
    66721 cgcgggtaat tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga
    66781 tgatttacgt gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc
    66841 ggaagatgat attcagctat ttccgctact gcgtaatctg acgctggtag ccggaatcaa
    66901 ctggccaagc cgcgttgctg attaccgcga taatatggcg aaacagacac aaatcaattt
    66961 gttatcatca atggcgattt aatcctgacc cgccgcggca gcctgtattc gcgggttgcc
    67021 gccgttttca gttttttctg ctggcgcgtt tatcgctggt gacacatgct gcgttgtgtc
    67081 aggatattga cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg
    67141 cattgatcgt cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg
    67201 aacaagaaat taaccagtcg cttgcgaaac ataataattt ctcaaaagat atcggtttac
    67261 ccggcgtggc tgacgcccat attgttctga caaacctgac cagccaaatt ggtcgcgaag
    67321 agccgaataa ggttacccta accggagacg ccaatctgga catgaactcc ctgttcggta
    67381 gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag
    67441 gtgcgatctt cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc
    67501 aaacggtgat gcaaacgttg ctcccctatt tgaaccaggc attacgcaat tactttaacc
    67561 agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg cgaagcaatg gcgaagaaac
    67621 tggcgaaagg cattgaagtg aagccaggcg aaattgtcat tccgtttact gattaatcac
    67681 gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg
    67741 tgtccggcaa aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg
    67801 tattaaagat ccgccgacca gacgactggc accttcacct ccgcgatggc ggcatgttaa
    67861 aaactgtcgt gccgtatacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg
    67921 ctccgcccgt gaccaccgtt gaggctgccg tggcttatcg ccagcgtatt cttgacgccg
    67981 tacctgccgg gcacgatttc accccgctga tgacctgtta tttgacagat tcactagacc
    68041 ctaatgagct tgagcgtgga tttaacgaag gcgtgttcac cgctgccaaa ctttacccgg
    68101 caaacgcaac cactaactcc agtcacggcg tgacgtcagt tgacgcaatc atgccggtac
    68161 ttgagcgcat ggaaaaaatc ggtatgccgt tactggtgca tggtgaagtg acacatgcag
    68221 atatcgacat ttttgatcgt gaagcgcgct ttattgaaag cgtgatggaa ccgctgcgcc
    68281 agcgcctgac tgcgctgaaa gtcgtttttg agcacatcac caccaaagat gctgccgact
    68341 atgtccgtaa cggaaatgaa cggctggctg ccaccatcac tccgcagcat ctgatgttta
    68401 accgcaacca tatgctggtt ggtggcgtgc gtccacatct gtattgtcta cccatcctca
    68461 aacgcaatat tcaccaacag gcattgcgtg aactggtcgc cagcggtttt aatcgcgtat
    68521 tcctcggtac ggattctgcg ccgcatgcac gtcatcgcaa agagagtagc tgcggctgcg
    68581 cgggctgctt caatgcccca accgcgctgg gcagttacgc taccgtcttt gaagagatga
    68641 atgctctgca gcactttgaa gcattctgtt ctgtaaacgg cccacagttc tatggcttgc
    68701 cggtcaacga cacattcatc gaactggtcc gcgaagagca acaggttgct gaaagcatcg
    68761 cattgactga tgacacactg gtgccattcc tcgccgggga aacggtacgc tggtccgtta
    68821 aacaataaaa atcctagctt cctgttgtca ttaggttatt ttacctgtat aaataaccag
    68881 tatattcaac agggggctat tatgcgaatt gaagtcacca tagcgaaaac ttctccattg
    68941 ccagctgggg ctattgacgc cctggctggc gaactttccc gccgtattca gtatgcgttt
    69001 cctgataatg aaggccacgt atcggtacgt tatgccgcag cgaataattt atcggttatt
    69061 ggcgcaacaa aagaagataa acagcgcatt agcgaaattc tccaggaaac gtgggaaagc
    69121 gccgatgact
//
LOCUS       DAOTRK010000009        63622 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.009, whole genome shotgun sequence.
ACCESSION   DAOTRK010000009 DAOTRK010000000
VERSION     DAOTRK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.009"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(171..926)
                     /gene="mlaA"
                     /locus_tag="QIE41_000640"
     CDS             complement(171..926)
                     /gene="mlaA"
                     /locus_tag="QIE41_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDS3529568.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1220..2152
                     /locus_tag="QIE41_000641"
     CDS             1220..2152
                     /locus_tag="QIE41_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDS3529569.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2228..2302
                     /locus_tag="QIE41_000642"
     tRNA            2228..2302
                     /locus_tag="QIE41_000642"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2261..2263,aa:Arg,seq:cct)
     gene            2512..2664
                     /locus_tag="QIE41_000643"
     CDS             2512..2664
                     /locus_tag="QIE41_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529570.1"
                     /translation="MHSLMNSQARDTRDRSLNDDNVLEISEPRIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(2744..3991)
                     /locus_tag="QIE41_000644"
     CDS             complement(2744..3991)
                     /locus_tag="QIE41_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529571.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4053..4976)
                     /locus_tag="QIE41_000645"
     CDS             complement(4053..4976)
                     /locus_tag="QIE41_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDS3529572.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHAEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELESEK"
     gene            5192..6625
                     /gene="cscA"
                     /locus_tag="QIE41_000646"
     CDS             5192..6625
                     /gene="cscA"
                     /locus_tag="QIE41_000646"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDS3529573.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(6633..7691)
                     /locus_tag="QIE41_000647"
     CDS             complement(6633..7691)
                     /locus_tag="QIE41_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDS3529574.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPKDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            7871..8089
                     /locus_tag="QIE41_000648"
                     /pseudo
     CDS             7871..8089
                     /locus_tag="QIE41_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8107..9435
                     /gene="dsdA"
                     /locus_tag="QIE41_000649"
     CDS             8107..9435
                     /gene="dsdA"
                     /locus_tag="QIE41_000649"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDS3529575.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATRGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRNAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(9543..11081)
                     /gene="emrY"
                     /locus_tag="QIE41_000650"
     CDS             complement(9543..11081)
                     /gene="emrY"
                     /locus_tag="QIE41_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDS3529576.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVMSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11081..12244)
                     /gene="emrK"
                     /locus_tag="QIE41_000651"
     CDS             complement(11081..12244)
                     /gene="emrK"
                     /locus_tag="QIE41_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDS3529577.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            12660..13274
                     /gene="evgA"
                     /locus_tag="QIE41_000652"
     CDS             12660..13274
                     /gene="evgA"
                     /locus_tag="QIE41_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDS3529578.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            13279..16872
                     /gene="evgS"
                     /locus_tag="QIE41_000653"
     CDS             13279..16872
                     /gene="evgS"
                     /locus_tag="QIE41_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDS3529579.1"
                     /translation="MKFLPYIILLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALTEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKDSVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYQVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     DIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPMTLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAPIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILKLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(16928..18073)
                     /gene="yfdE"
                     /locus_tag="QIE41_000654"
     CDS             complement(16928..18073)
                     /gene="yfdE"
                     /locus_tag="QIE41_000654"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDS3529580.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(18147..19091)
                     /gene="yfdV"
                     /locus_tag="QIE41_000655"
     CDS             complement(18147..19091)
                     /gene="yfdV"
                     /locus_tag="QIE41_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDS3529581.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(19160..20854)
                     /gene="oxc"
                     /locus_tag="QIE41_000656"
     CDS             complement(19160..20854)
                     /gene="oxc"
                     /locus_tag="QIE41_000656"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDS3529582.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNDIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLEIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(20908..22158)
                     /gene="frc"
                     /locus_tag="QIE41_000657"
     CDS             complement(20908..22158)
                     /gene="frc"
                     /locus_tag="QIE41_000657"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDS3529583.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(22670..23302)
                     /locus_tag="QIE41_000658"
     CDS             complement(22670..23302)
                     /locus_tag="QIE41_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDS3529584.1"
                     /translation="MKRLIMATMVTAVLASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASIGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            23598..23873
                     /locus_tag="QIE41_000659"
     CDS             23598..23873
                     /locus_tag="QIE41_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529585.1"
                     /translation="MKVNLMLFSLFLLVSIMACNVFAFSISGGGSETSYKETEKTSMM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPPFPTKANYLIHPVR"
     gene            complement(23950..24192)
                     /locus_tag="QIE41_000660"
     CDS             complement(23950..24192)
                     /locus_tag="QIE41_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDS3529586.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVISAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            24545..25465
                     /gene="lpxP"
                     /locus_tag="QIE41_000661"
     CDS             24545..25465
                     /gene="lpxP"
                     /locus_tag="QIE41_000661"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDS3529587.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKSEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            25821..25892
                     /gene="ypdK"
                     /locus_tag="QIE41_000662"
     CDS             25821..25892
                     /gene="ypdK"
                     /locus_tag="QIE41_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDS3529588.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(25957..27195)
                     /gene="alaC"
                     /locus_tag="QIE41_000663"
     CDS             complement(25957..27195)
                     /gene="alaC"
                     /locus_tag="QIE41_000663"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDS3529589.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISHWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            27572..29269
                     /gene="ypdA"
                     /locus_tag="QIE41_000664"
     CDS             27572..29269
                     /gene="ypdA"
                     /locus_tag="QIE41_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDS3529590.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            29284..30018
                     /gene="ypdB"
                     /locus_tag="QIE41_000665"
     CDS             29284..30018
                     /gene="ypdB"
                     /locus_tag="QIE41_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDS3529591.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            30031..30888
                     /locus_tag="QIE41_000666"
     CDS             30031..30888
                     /locus_tag="QIE41_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3529592.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(30891..33386)
                     /gene="ptsP"
                     /locus_tag="QIE41_000667"
     CDS             complement(30891..33386)
                     /gene="ptsP"
                     /locus_tag="QIE41_000667"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDS3529593.1"
                     /translation="MLTIQFLCPLPNGIHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLILLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASVIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPDDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIITTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(33411..34448)
                     /gene="ypdE"
                     /locus_tag="QIE41_000668"
     CDS             complement(33411..34448)
                     /gene="ypdE"
                     /locus_tag="QIE41_000668"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDS3529594.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(34448..35533)
                     /gene="ypdF"
                     /locus_tag="QIE41_000669"
     CDS             complement(34448..35533)
                     /gene="ypdF"
                     /locus_tag="QIE41_000669"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDS3529595.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHKLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGFGDNFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(35548..36795)
                     /locus_tag="QIE41_000670"
     CDS             complement(35548..36795)
                     /locus_tag="QIE41_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDS3529596.1"
                     /translation="MAIKKRSATVVPGASGAAAAIKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(36817..37143)
                     /locus_tag="QIE41_000671"
     CDS             complement(36817..37143)
                     /locus_tag="QIE41_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDS3529597.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(37362..38327)
                     /gene="glk"
                     /locus_tag="QIE41_000672"
     CDS             complement(37362..38327)
                     /gene="glk"
                     /locus_tag="QIE41_000672"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDS3529598.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWISLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            38531..39787
                     /locus_tag="QIE41_000673"
     CDS             38531..39787
                     /locus_tag="QIE41_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDS3529599.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIAILTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            39902..40228
                     /locus_tag="QIE41_000674"
     CDS             39902..40228
                     /locus_tag="QIE41_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDS3529600.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(40370..41608)
                     /locus_tag="QIE41_000675"
     CDS             complement(40370..41608)
                     /locus_tag="QIE41_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDS3529601.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            41944..43146
                     /gene="nupC"
                     /locus_tag="QIE41_000676"
     CDS             41944..43146
                     /gene="nupC"
                     /locus_tag="QIE41_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDS3529602.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(43197..45386)
                     /locus_tag="QIE41_000677"
     CDS             complement(43197..45386)
                     /locus_tag="QIE41_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDS3529603.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYNISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(45594..45669)
                     /locus_tag="QIE41_000678"
     tRNA            complement(45594..45669)
                     /locus_tag="QIE41_000678"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45634..45636),aa:Ala,seq:ggc)
     gene            complement(45709..45784)
                     /locus_tag="QIE41_000679"
     tRNA            complement(45709..45784)
                     /locus_tag="QIE41_000679"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45749..45751),aa:Ala,seq:ggc)
     gene            46005..46364
                     /locus_tag="QIE41_000680"
     CDS             46005..46364
                     /locus_tag="QIE41_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDS3529604.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            46366..46758
                     /locus_tag="QIE41_000681"
     CDS             46366..46758
                     /locus_tag="QIE41_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDS3529605.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(46809..48224)
                     /gene="gltX"
                     /locus_tag="QIE41_000682"
     CDS             complement(46809..48224)
                     /gene="gltX"
                     /locus_tag="QIE41_000682"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDS3529606.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            48483..48558
                     /locus_tag="QIE41_000683"
     tRNA            48483..48558
                     /locus_tag="QIE41_000683"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48516..48518,aa:Val,seq:tac)
     gene            48603..48678
                     /locus_tag="QIE41_000684"
     tRNA            48603..48678
                     /locus_tag="QIE41_000684"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48636..48638,aa:Val,seq:tac)
     gene            48683..48758
                     /locus_tag="QIE41_000685"
     tRNA            48683..48758
                     /locus_tag="QIE41_000685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48716..48718,aa:Lys,seq:ttt)
     gene            complement(48869..49753)
                     /gene="xapR"
                     /locus_tag="QIE41_000686"
     CDS             complement(48869..49753)
                     /gene="xapR"
                     /locus_tag="QIE41_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDS3529607.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPA
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALGDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(49794..49952)
                     /locus_tag="QIE41_000687"
     CDS             complement(49794..49952)
                     /locus_tag="QIE41_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HDS3529608.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(50005..51261)
                     /gene="xapB"
                     /locus_tag="QIE41_000688"
     CDS             complement(50005..51261)
                     /gene="xapB"
                     /locus_tag="QIE41_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDS3529609.1"
                     /translation="MSIALRLKVMFFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVSDPDTMFWVMLIN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYVASGASLLLSAYALTLPKIPVAEKKSATSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMMMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(51321..52154)
                     /gene="xapA"
                     /locus_tag="QIE41_000689"
     CDS             complement(51321..52154)
                     /gene="xapA"
                     /locus_tag="QIE41_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDS3529610.1"
                     /translation="MSQARFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDADYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHTQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            52403..53167
                     /locus_tag="QIE41_000690"
     CDS             52403..53167
                     /locus_tag="QIE41_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529611.1"
                     /translation="MKKHLFTLTLSSVLAIPAVTHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSYITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(53205..54131)
                     /locus_tag="QIE41_000691"
     CDS             complement(53205..54131)
                     /locus_tag="QIE41_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3529612.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRGQGRALMAGREGDPLYQI"
     gene            54221..55219
                     /locus_tag="QIE41_000692"
     CDS             54221..55219
                     /locus_tag="QIE41_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDS3529613.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKHQTEQLQAQQESRANKA"
     gene            complement(55216..55434)
                     /locus_tag="QIE41_000693"
     CDS             complement(55216..55434)
                     /locus_tag="QIE41_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDS3529614.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(55436..57451)
                     /gene="ligA"
                     /locus_tag="QIE41_000694"
     CDS             complement(55436..57451)
                     /gene="ligA"
                     /locus_tag="QIE41_000694"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDS3529615.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGILEGGELPNTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLTQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(57522..58520)
                     /gene="zipA"
                     /locus_tag="QIE41_000695"
     CDS             complement(57522..58520)
                     /gene="zipA"
                     /locus_tag="QIE41_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDS3529616.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            58750..59511
                     /gene="cysZ"
                     /locus_tag="QIE41_000696"
     CDS             58750..59511
                     /gene="cysZ"
                     /locus_tag="QIE41_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDS3529617.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            59696..60667
                     /gene="cysK"
                     /locus_tag="QIE41_000697"
     CDS             59696..60667
                     /gene="cysK"
                     /locus_tag="QIE41_000697"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDS3529618.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            61051..61308
                     /gene="ptsH"
                     /locus_tag="QIE41_000698"
     CDS             61051..61308
                     /gene="ptsH"
                     /locus_tag="QIE41_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDS3529619.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            61353..63080
                     /gene="ptsI"
                     /locus_tag="QIE41_000699"
     CDS             61353..63080
                     /gene="ptsI"
                     /locus_tag="QIE41_000699"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDS3529620.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            63121..>63622
                     /gene="crr"
                     /locus_tag="QIE41_000700"
     CDS             63121..>63622
                     /gene="crr"
                     /locus_tag="QIE41_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDS3529621.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRI"
BASE COUNT        16773 a        16023 c        14894 g        15932 t
ORIGIN      
        1 cagttttttg atttttcaac gactgaatat ctgaaatagt caatgacgaa catcagtatt
       61 tttacatcat tacgtccggg tcattgtcgg tttatctcct tttacttgtg gtaattgagg
      121 gataacaaca aaaaaggtga gttttgcgac tcaccttttt tatttgtttc ttattcagaa
      181 tcgatatctt ttaaatcatc ctgaatcgct tgcgcgttcg gattttcctg cggtttgagt
      241 tcgccgccat tagcgatgaa atcatgacgc tggaagtacg cttcgcgcac cataatgtaa
      301 ggatcggacg actgacgcag cagaccgtcg gaatccagca gctgtgcgcg ggtttcgata
      361 ccttcaagcg tccatttacc cacagacatc ggccaggtca accaggaaag aaccgggtaa
      421 agaccatccg ccatatcacc accgtcatca cgcagcgtga agctaccgta gaacggtaac
      481 tgaacgtatg gcccataacc cacaccataa tgaccaagcg tactaccgaa gcggtgaggt
      541 tcagtccgtt gcagtttcgg gttcgccatc cctgcaacat caataaagcc gcccatcccc
      601 aaaatggtgt tcaggaaaaa gcgggtaaag tggaccatcc cctgataagg gtcgccctgc
      661 aagaagtagt taaccatcac cgcaggttct tcaaggttgc cagtaaagtt gctcaaaccg
      721 ttacgcgccg gttgcggaac ataatcacgc caggcgacag cgactggtcg aacaatatac
      781 gggtctaata cattgaagtt gaagttgtac atggtgcggt tgaacccttc taacgggtca
      841 gaacgccctt gctgatctgt accggaactc gcacacccca ccagaagcgt agttcccaga
      901 gcaagcgccg acaggcgaag cttcataaat gtctccctgt ttttttatgg cttatgcagt
      961 ttgccatcca tgacggaacg ataccgtatc cgcctgttta ggtgtgggcg attgtaacag
     1021 cacgtcaact gatgtccaga cgccctgatt tgctgatttg atcatagcct ggtaatcgcc
     1081 gccctgtagg ctacttgatt ctatagaaac agaaaaaggc aaacgcggcc ttttctacat
     1141 tttcagagta actcccgcca gttgcgagca aaaaagccgc tacgctttag ctatacgtgc
     1201 taattcaaga gaagagacca tggacaacga caaaattgat caacacagcg acgaaattga
     1261 agttgagagc gaagaaaaag agcgcggcaa aaaaatagaa atagatgaag atcgactccc
     1321 ctcccgggcg atggcaattc atgagcatat ccgccaggat ggtgaaaaag agctggaacg
     1381 cgacgcaatg gcgctactgt ggtcagccat tgcggcgggt ctgtcgatgg gcgcttcgct
     1441 actggcaaaa gggatatttc atgtcgaact ggaaggcgtg ccgggcagct tcttgctgga
     1501 gaatctcggt tatacctttg gttttattat cgtcattatg gcccgccagc aattatttac
     1561 cgaaaacacc gtgactgcgg tactacccgt catgcaaaaa ccgacaatga gcaacgttgg
     1621 cttacttatg cggttatggg gcgtcgtgct gctgggtaat attctcggga caggtattgc
     1681 ggcgtgggca tttgaatata tgccaatctt caatgaagaa actcgcgatg catttgtcaa
     1741 aatcggcatg gatgtgatga agaacacccc cagcgagatg tttgccaacg cgatcatttc
     1801 cggctggctg atcgccacta tggtatggat gtttcctgca gcgggtgcgg caaagattgt
     1861 ggtgattata ttgatgacct ggcttattgc actgggtgac accactcaca tcgttgtcgg
     1921 ttctgtagaa atcctctatc tggtgtttaa cggcacgctg cactggagcg atttcatctg
     1981 gcccttcgca ctacctactt tagcggggaa catctgcggc ggcaccttta tcttcgcgtt
     2041 aatgagtcat gcacagattc gtaacgacat gagcaacaag cgcaaagcag aagcacgcca
     2101 aaaagcagaa cgtgcggaaa acattaagaa aaatgataaa aacccggcat aaatggcgag
     2161 ggtttaagca atcgagcggc agcgtactta ccccgcagtc cattagcggg tatactcatg
     2221 ccgcattgtc ctcttagtta aatggatata acgagcccct cctaagggct aattgcaggt
     2281 tcgattcctg caggggacac cagataccct tctaacaata tctaccttca ccccgtaaaa
     2341 gatgggtttg gcagcacact tgccctatat ctactcattt ttactgcaac aggttgaaat
     2401 ctcagcactg tcagaaagcg ctgatgacta aacagccctg ggccgggcga tgtaaccatc
     2461 atacagaatc ctgatagcga aatatggcgt gactcgatac ttcactctgc aatgcattcc
     2521 ttgatgaatt cgcaggcccg tgatactcgg gacaggtcgc tgaatgacga caatgtcctg
     2581 gaaatcagcg aaccgcgtat ccggagtaca tttgagcgac tgtaccagaa catgaatgag
     2641 gcgtttggat taggtgatta ttagttgggc taagcatttt tgtattatta ttttccggtt
     2701 gagggatatg gagatatcga caacaaccgg aaaaagttta cgtctatatt gctgaaggta
     2761 caggcgtttc cataactatt tgctcgcgtt ttttactcag gaagaaaatg ccaaatagca
     2821 acatcaggca gacaataccc gaaattgcga agaaaactgt ctggtagcct gcgtggtcaa
     2881 agagtatccc agtcggcgtt gaaagcagca caatcccaag cgaactggca atttgaaaac
     2941 caatcagaaa gatcgtcgac gacaggcgct tatcaaagtt tgccacgctg tatttgaaga
     3001 cggatatgac acaaagtgga acctcaatgg catgtaacag cttcactaat gaaataatcc
     3061 aggggttaac gaacaacgcg caggaaagga tacgcaacgc cataatcaca acaccgataa
     3121 gtaatgcatt ttttggccct acccgattca caaagaaagg aataatcgcc atgcacagcg
     3181 cttcgagtac cacctggaat gagttgagat aaccatacag gcgcgttcct acatcgtgtg
     3241 attcgaataa acctgcataa aagacaggaa agagttgttg atcaaaaatg ttatagaaag
     3301 accacgtccc cacaataaat atgacaaaaa cccagaagtt tcgatccttg aaaactgcga
     3361 taaaatcctc tttttttacc cctcccgcat ccgccgctat gcactggtga tccttatctt
     3421 taaaacgcat gttgatcatc ataaatacag cgccaaatag cgagaccaac cagaagttga
     3481 tatggggact gatactaaaa aatataccgg caaagaacgc gccaatagca tagccaaaag
     3541 atccccaggc gcgcgctgtt ccatattcga aatgaaaatt tcgcgccatt ttttcggtga
     3601 agctgtcaag caaaccgcat cccgccagat accccaggcc aaaaaagagc gcccccagaa
     3661 ttagacctac agaaaaattg ctttgcagta acggttcata aacgtaaatc ataaacggtc
     3721 cggtcaagac cagaatgaaa ctcatacacc agatgagcgg tttcttcaga ccgagtttat
     3781 cctgaacgat gccgtagaac atcataaata gaatgctggt aaactggttg accgaataaa
     3841 gtgtacctaa ttccgtccct gttaatccta gatgtccttt cagccaaata gcgtataacg
     3901 accaccacag cgaccaggaa ataaaaaaga gaaatgagta actggatgca aaacgatagt
     3961 acgcatttct gaatggaata ttcagtgcca taattacctg cctgtcgtta aaaaattcat
     4021 gtcctattta gagataagag cggcctcgcc gtttacttct cactttccag ttcttgtcga
     4081 catggcagcg ctgtcattgc ccctttcgct gttactgcaa gcgctccgca acgttgagcg
     4141 agatcgataa ttcgtcgcat ttctctctca tctgtagata accccgtaga ggacagacct
     4201 gtgagtaacc cggcaacgaa cgcatctccc gccccagtgc tatcgacaca attcacagac
     4261 attccagcaa aatggtgaac ttgtcctcga taacagacca ccaccccttc tgcaccttta
     4321 gtcaccaaca gcatggcgat ctcatactct tttgccaggg cgcatatatc ccgatcgttc
     4381 tgtgtttttc cactgataag tcgccattct tcttccgaga gcttgacgac atccgccagt
     4441 tgtagcgcct gccgcaaaca caagcggagc aaatgctcgt cttgccatag atcttcacga
     4501 atattgggat cgaagctgac aaaacctccg gcatgccgga tcgccgtcat cgcagtaaat
     4561 gcgctggtac gcgaaggctc ggcagacaac gcaattgaac agagatgtaa ccattcggca
     4621 tgtcgccagc agggcaagtc tgtcgtctct aaaaaaagat cggcactggg gcggaccata
     4681 aacgtaaatg aacgttctcc ttgatcgttc agatcgacaa gcaccgtgga tgtccggtgc
     4741 cattcatctt gcttcagata cgtgatatcg acaccctcag ttagcagcgt tctttgcatt
     4801 aacgcaccaa aaggatcatc accgacccga cctataaacc cacttgttcc gcctaatctg
     4861 gcgattccca ccgcaacgtt agctggcgcg ccgccaggac aaggcagtag ccgcccgtct
     4921 gattctggca agagatctac gaccgcatcc cctaaaaccc atactttggc tgacattttt
     4981 ttcccttaaa ttcatctgac ttacgcatag tgataaacct ctttttcgca aaatcgtcat
     5041 ggatttacta aaacatgcat attcgatcac aaaacgtcat agttaacgtt aacatttgtg
     5101 atattcatcg catttatgaa agtaagggac tttattttta taaaagttaa cgttaacaat
     5161 tcaccaaatt tgcttaacca ggatgataaa aatgacgcaa tctcgattac atgcggcgca
     5221 aaacgcacta gcaaaacttc acgagcgccg aggtaacact ttctatcccc attttcacct
     5281 cgcgcctcct gccgggtgga tgaacgatcc aaacggcctg atctggttta acgatcgtta
     5341 tcacgcgttt tatcaacatc acccgatgag cgaacactgg gggccaatgc actggggaca
     5401 tgccaccagc gacgatatga tccactggca gcatgagcct attgcgctag cgccaggaga
     5461 cgagaatgac aaagacgggt gtttttcagg tagtgctgtc gatgacaatg gtgtcctctc
     5521 acttatctac accggacacg tctggctcga tggtgcaggt aatgacgatg caattcgcga
     5581 agtacaatgt ctggctacca gtcgggatgg tattcatttc gagaaacagg gtgtgatcct
     5641 cactccacca gaaggcatca tgcacttccg cgatcctaaa gtgtggcgtg aagccgacac
     5701 atggtggatg gttgtcgggg cgaaagatcc aggcaacacg gggcagatcc tgctttatcg
     5761 cggcagttca ttgcgtgaat ggaccttcga tcgcgtactg gcccacgctg atgcgggtga
     5821 aagctatatg tgggaatgtc cggacttttt cagccttggc gatcagcatt atctgatgtt
     5881 ttccccgcag ggaatgaatg ccgagggata cagttatcga aatcgctttc aaagtggcgt
     5941 aatacccgga atgtggtcgc caggacgact ttttgcacaa tccgggcatt ttactgaact
     6001 tgataacggg catgactttt atgcaccaca aagctttgta gcgaaggatg gtcggcgtat
     6061 tgttatcggc tggatggata tgtgggaatc gccaatgccc tcaaaacgtg aaggctgggc
     6121 aggctgcatg acgctggcgc gcgagctatc agagagcaat ggcaaacttc tacaacgccc
     6181 ggtacacgaa gctgagtcgt tacgccagca gcatcaatct atctctcccc gcacaatcag
     6241 caataaatat gttttgcagg aaaacgcgca agcagttgag attcagttgc agtgggcgct
     6301 gaagaacagt gatgccgaac attacggatt acagctcggc actggaatgc ggctgtatat
     6361 tgataaccaa tctgagcgac ttgttttgtg gcggtattac ccacacgaga atttagatgg
     6421 ctaccgtagt attcccctcc cgcagggtga catgctcgcc ctaaggatat ttatcgatac
     6481 atcatccgtg gaagtattta ttaacgacgg ggaggcggtg atgagtagcc gaatatatcc
     6541 gcagccagaa gaacgggaac tgtcgctcta tgcctcccac ggagtggctg tgctgcaaca
     6601 tggagcactc tggcaactgg gttaacataa tatcaggtgg aacaacggat caacagcggg
     6661 caagggatcc gcgtcactct tcccccttca cgaccttcaa taatatgcaa tgcagcttcc
     6721 cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg gcggtaaaaa cagatgcccg
     6781 acgccaacca gattatcaaa gcccattacg gcgacatcct tcgggatacg tacccccttc
     6841 gccaaaagaa cctgataagc cacaaaggct gcgcgatcgt taccacatat cagaacatca
     6901 aaatctggtt tgcccgattt gaagtggtca ttgagtaaac ttgcgagatc ggtgtagtga
     6961 tcatcacctg ttgccatgtg aaattgtttc acctcagcca gatctcgtcc agcatcacgc
     7021 caggcctgct caaatccctg ccgacgatac cctgttgcca acgcactttc cggtagccag
     7081 aagcataacg gttgacgata gcccgccgcg agcaaatgct gtgttgattc atattgtgca
     7141 gtgtaatcat cagggatata actgggtaac gctgggtcat ccgccacaca gttcgccaat
     7201 acaatatttt caccatacag agactcaggc agcgtgatat gtcgcagccc cattgtagta
     7261 tagataatgc catccggacg gtgggcaagc agctgacgtg ccgcgcgggc agcgtcatct
     7321 tcagaaaaaa tattgattaa aaaactattc cagccgaact cgctggcggt ttgctcaatg
     7381 gcaagcagaa tatcaacaga gaaaggagtg gtagcagtgt cctgcgccag cacggcgaga
     7441 gtcgacggct tacgtccttg agcgcgcatc ttacgggcgg aaagatcagg aacataattc
     7501 agggtctgga ttgcctgcaa tacgcgatca cgcgttgcag gacgcacaga ttctgcatta
     7561 tgcatcaccc gggagactgt catcatcgac actcccgcca ggcgtgcgac atcctttaat
     7621 gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt agcagaaaca gacgtcactg
     7681 gcgagatcca acgatattct cgacgcccta tcacccgaca cggcaataca ataaaaaata
     7741 acaataattc ccggacaatt gtacccaatt ccgcctctgt tctcgccaac gagtaaccat
     7801 gtaggcaagt aggtaaaaca tgccaactgg catcgccaat ctggagaaga aaatataaat
     7861 atggatcatt gtgctgccgc tgtatcccga catcagcccg gaaattattg cgattgctat
     7921 cggttcaggt gcgattggct gcacgatcgt tacggactcg cttttctggc tggcgaagca
     7981 atattgcggc gctacgctca atgaaacatt taaatactat acgacagcga catttatcgc
     8041 ttcagtcgtc gctctggcgg gcacattcct gctgtcattt atcatctaag cgcaaagaga
     8101 cgtactatgg aaaacgctaa aatgaattcg ctcatcgccc agtatccatt ggtaaaggat
     8161 ctggttgctc ttaaagaaac cacctggttt aatcctggca cgacctcatt ggctgaaggt
     8221 ttaccttatg ttggcctgac cgaacaggat gttcaggacg cccatgcgcg cttatcccgt
     8281 tttgcaccct atctggcaaa agcatttcct gaaactgctg ctactagggg gattattgaa
     8341 tcagaactgg ttgccattcc ggctatgcaa aaacggctgg aaaaggaata tcaacaaccg
     8401 atcagcgggc aactgctact gaaaaaagat agccatttgc ccatttccgg ctccataaaa
     8461 gcacgcggcg ggatttatga agtcctggca catgcagaaa aactggctct ggaagcgggg
     8521 ttgctgacgc ttgaagatga ctacagcaaa ctgctttctc cggagtttaa acagttcttt
     8581 agccaataca gcattgctgt gggctcaacc ggaaatctgg ggttatcaat cggcattatg
     8641 agcgcccgca ttggctttaa ggtgacagta catatgtctg ctgatgcccg ggcatggaaa
     8701 aaagcgaaac tgcgcagcca tggcgttacg gtcgtggaat atgagcaaga ttatggtgtt
     8761 gcagtcgagg aaggacgtaa cgcagcgcag tctgacccga actgtttctt tattgatgac
     8821 gagaattccc gcacgttgtt ccttgggtat tccgtcgcag gccagcgtct taaagcgcaa
     8881 tttgccgagc aaggtcgtat cgtcgatgct gataaccctc tgtttgtcta tctgccgtgt
     8941 ggtgttggcg gtggtcctgg tggcgtcgca ttcgggctta aactggcatt tggcgatcat
     9001 gttcactgct tttttgccga accaacgcac tccccttgta tgctgttagg cgtccataca
     9061 ggattacacg atcagatttc tgttcaggat attggtatcg acaaccttac cgcagcggat
     9121 ggccttgcag ttggtcgcgc atcaggcttt gtcgggcggg caatggagcg tctactggat
     9181 ggcttctata cccttagcga tcaaaccatg tatgacatgc ttggctggct ggcgcaggaa
     9241 gaaggcattc gtcttgaacc ttcggcactg gcgggtatgg ccgggcctca gcgcgtgtgc
     9301 gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag aacaactgcg taatgccact
     9361 catctggtgt gggcgacggg aggtggaatg gtgccggaag aagagatgga gcaatatctg
     9421 gcaaaaggcc gctaattaat tttcaattca gcatcgccat cctttccctg ggtgagcgat
     9481 gctgccgatg gcgcagactt aaggtccctg gtcttacctg ctataacccc ctccttttat
     9541 catcacccaa cgcctttcgc tgtaaacggc ggtttcgcaa accaaaccaa aactgtaagt
     9601 aagataaaag caatagccgc catacggaaa atctcatttg cagaaattga aagtgactgc
     9661 tgagttattt cattattaat ttcattaaga acccctgaaa gcgaaccata gtatttatcc
     9721 ataatttgtg acgatgaatt aaacacgggg ttaaattgat cgatggttgc tgtcaactga
     9781 ctatggtgta acgattcgcg tcgtccccac agcgtcattg tcaacgacgt accaactgat
     9841 cctgacaagg tacgaaaaaa attactcata ctcgaggcat tggcaaattt attatctggc
     9901 aaacctgaaa acgaaatcgt tgttaagggt aaaaagaaac aggcgacggc gaatccctga
     9961 aaaaactgtg gcaaaatgat gcctgtaaaa tcaatcgttg gcataaatgt cacagaacgc
    10021 cagtaatagc aaaccgcata cattaaaaaa ctaaatgtca ccaacaaccg catatcaatt
    10081 ttgttgccat aacgtcctat caaaggtgat attaatagcg gcatgatgcc gatgggcgca
    10141 taggcaagtc cggcccatat cgcattatac cccatcgttt cctggagtaa ctgcggcata
    10201 aggactatcg ccccagagta aaataaatat gcgcatgtga tactcacaat accaatggtg
    10261 aaattacggg acttaaacaa actgagatca agaatcgggt tctctgaggt cgactcccaa
    10321 atgactaaag aaatcagaga aataactgac attactgtta gtattattat tgtactcgag
    10381 ttgaaccaat ccagatcgcg ccctttatca agcattatct gcaagccacc aacaccgagc
    10441 actaacaggg tcagtcctgg tagattcatt ttgaccggtg aagtctcagt ttctcttcct
    10501 ttaagtaagg ttaagcataa tgtcagaacg ataatcccca tagggacatt gattaaaaat
    10561 atccaacccc agctaaagtt atcacaaata taaccgccca atatcggccc acatatcggc
    10621 gcgataatca cggtcataga ccataatgcc agagcaaatg ttcttttttc tggtggataa
    10681 ttccttaata ataaactctg tgacagtgga attaacggcc ccgccattaa tccctgaacg
    10741 actctaaaaa atatcagcac atcaagattg gtcgataaac tacacattaa ggaagacagc
    10801 gaaaaaaaag tgactgaaag taaaaataat cttaattcgc ctattctttg tgccagcctg
    10861 ccggtaacag ggatcgcaat ggcatttgct acgccaaacg aagtgataac ccaggtgcct
    10921 tcgtctgttg atgctcccag aaagccagat attgtcggta ttgcgacgtt agaaatagtg
    10981 gaatccaaca tttgcataaa cgtcgctaat gacaatgcaa tagtgacgca ccataacgtc
    11041 ccaccggtta atggtgccgg agttgattta gtgattgcca ttaaagttgt ccattatgcg
    11101 aaataatgtt gctaatttct ttttctatcg gactggtatc gataactaaa gccttactgg
    11161 tataagcagg catggacgtc acggttgaag ccagctcagg catctcggca atgtcttcgt
    11221 tcttcgtatc aatagttgct gtcatcgata aaccaatacg taagggatgt tccatgagtt
    11281 cttttggatc aagagaaact tcaaccggta cacgctgaac gattttgatc cagttccctg
    11341 tcgcattttg tgcaggtaat aaggagaacg cattgccggt tcccatattg atccctgtca
    11401 cccgaccatg aaacacaaca ttttcaccat aaagatcgct gataatattg accgattgac
    11461 caatccgtac atccgtgagt tgtgtttctt taaagttagc attaacccac atttgacgtg
    11521 ccggtacgac agccattaac gattgtccgg gactcactgt ttcgccgacc tgaacacttc
    11581 gctgggcaat gtagccggta accggactct taatatccgt acgtttaagc gccaaccagg
    11641 cttctttggt tgcatccgca gcttcaacga cttgtggctg acggtttaat ggtgtgttca
    11701 ttactaaagc tttattcgct ttataagcct gaatagcagc attcaatgcc gctttgctac
    11761 ttattaacgt atctttggta tgctccagcg tttcttttga aataaccccc tgcttcgcta
    11821 aaggtacacg acggttataa tcttctaaag attgttgata ctgaatacgt gctgaagcga
    11881 cttcggcact gtattgttta tcctgtaagt acagtttatt cgtttgccga acaatatttg
    11941 ccagattatt tttagcttta ttgagtgcga tagtggcatc agtcttatcc agtgagacta
    12001 aaatgtcacc ctgtcgaacg tagttcgtat ctttatgatt aacgacagtg acgctgcctg
    12061 aaacttgtgc agaaattgga tctgcattcc ccgtgacata ggcatcatct gtactaatca
    12121 tgtcttctaa ttccattgac caataggcat aggcacctga aaacgcaata aataaaacta
    12181 ccgccaataa agaaaagtat tttcttctgt tagaatgttt tttatttgaa ttaatctgtt
    12241 ccactattat ctctcatttc gcatagatga aatttatgaa ttgaatgaaa agagtattca
    12301 ttcttcatat caaacacagt gcatgtgttg cacattgtcc caggaacgtt ggaggcctaa
    12361 tttaaatgag gtgcaaaaaa aacaacagag gactaaaccg tggcttttgc aatacaattc
    12421 ttacgcatgt aggattagtg agaagactta tagtgctaat gtgaaactat aaatcatcgg
    12481 tacaatccct gattttattg ttgacatttc atttatgccg actatttata tggtatactt
    12541 gtcgaattat cttaaaggaa gctctaattt tcttattttt attgagaaaa tgagatgatg
    12601 ccttatgtct gtattactac agggagaagg gagatgcttc attgcaaagg gaataatcta
    12661 tgaacgcaat aattattgat gaccatcctc ttgctatcgc agcaattcgt aatttattga
    12721 tcaaaaacga tattgaaatc ttagcggagt tgactgaagg gggaagtgcc gttcagcggg
    12781 tggaaacact taaacctgat atcgtcatca ttgatgtcga tatccccgga gttaacggta
    12841 tccaggtgtt agaaacgctg aggaagcgcc aatatagcgg aattattatt atcgtctccg
    12901 ctaaaaatga ccatttttac gggaaacatt gtgctgatgc tggcgctaat ggattcgtga
    12961 gtaaaaaaga aggcatgaac aatatcattg cggctattga agctgcaaaa aatggctact
    13021 gctatttccc cttctctctc aaccggtttg ttggaagttt aacgtccgac cagcaaaaac
    13081 tcgactcctt atcgaaacaa gaaattagtg tcatgcgata tattcttgat ggcaaggata
    13141 ataatgacat tgcggaaaaa atgttcatca gcaacaaaac tgtcagcact tataaaagtc
    13201 gcctgatgga aaaattagaa tgtaaatcac tgatggatct ttacacattc gcacaacgta
    13261 acaaaatcgg ctaaccacat gaagttttta ccctatatta ttcttctctg ttgtggtctt
    13321 tggtcgacca taagtttcgc agacgaagat tacatcgaat atcgtggcat cagtagtaac
    13381 aaccgtgtca cgcttgatcc actacgtctg agcaacaagg aattacgttg gttagcgagc
    13441 aaaaaaaatc ttgtgattgc agtacacaag tcccaaacgg ctactttgtt gcataccgat
    13501 tcgcagcaac gggttcgtgg tattaatgct gattatttaa atcttttaaa aagagcgtta
    13561 aatatcaaat taacactccg ggaatacgca gatcatcaaa aagcaatgga cgcgcttaca
    13621 gaaggggaag tcgatatagt gttatcacat ttagttgctt cgccgcctct taatgatgac
    13681 attgctgcaa ccaaaccgct gataattacc tttccggcgc tggtcaccac ccttcacgat
    13741 tcaatgagac cgcttacctc atcaaaacca gtaaatattg ctcgggtagc aaattaccct
    13801 ccagatgagg taattcatca atcatttcca aaagcaacaa ttatctcttt tacaaattta
    13861 tatcaggcat tagcatccgt ctcagccgga cagaatgatt actttattgg tagtaacatt
    13921 attaccagca gtatgatttc ccgctatttc actcactcct taaatgtagt gaaatattat
    13981 aactcgccgc gtcaatataa ctttttctta accagaaaag attcagtcgt tcttaatgaa
    14041 gtactcaatc ggtttgttga tgctttaaca aatgaagttc gctatgaagt atcacaaaat
    14101 tggcttgata caggaaacct ggcctttctg aacaaaccat tagaactcac tgaacatgaa
    14161 aaacagtgga ttaagcagca tcccgattta aaggtgctgg aaaatcctta ctcgccaccc
    14221 tattctatga cagatgaaac tggctcagtt cggggcgtga tgggtgatat tcttaatatt
    14281 attaccttgc aaacaggttt aaatttttct ccgatcaccg tttcacacaa tattcatgcg
    14341 gggacacagc ttaaccccgg tggatgggat atattgcccg ccgctattta tagtgaagat
    14401 agagaaaata atgttttatt tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg
    14461 caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc cattccctat
    14521 tattatgagc ttcattcgca attaaaagag atgtatccgg aggtagagtg gataaaagtc
    14581 gataacgcca gcgctgcatt tcacaaggtc aaggaaggcg aacttgatgc tctggtcgcg
    14641 acacagttaa attcacgtta catgatcgac cattactatc ctaatgaact ttatcatttt
    14701 cttattcccg gcgttccgaa tgcatcgctt tcgttcgctt ttcctcgcgg agaaccggaa
    14761 cttaaggata ttattaataa agcactgaat gcaattcccc caagcgaagt tctgcgcctg
    14821 acggaaaaat ggattaaaat gcccaatgtg accattgaca catgggacct ctatagcgag
    14881 caattttata ttgtcacgac attatccgtt ttattagttg gcagtagcct tttatgggga
    14941 ttctacctgt tacgctcagt tcgtcgtcgt aaagtcattc agggtgattt agaaaaccaa
    15001 atatcattcc gaaaagcact ctcggattcc ttaccgaatc caacttatgt tgtaaactgg
    15061 caaggtaatg tcattagtca taatagtgct tttgaacatt atttcactgc ggattactac
    15121 aaaaatgcaa tgttaccatt agaaaacagt gactcaccct ttaaagatgt tttttctaat
    15181 gcgcatgaag tcacagcaga aacgaaagaa aatcgaacaa tatacacaca ggtatttgaa
    15241 attgataatg gcatcgagaa aagatgcatt aaccactggc atacattatg caatctgcca
    15301 gccagcgatc atgctgttta tatttgtggt tggcaagata ttaccgagac gcgtgattta
    15361 attcatgcac tcgaggtaga gagaaataaa gcgatcaatg caactgtcgc aaaaagccag
    15421 tttctggcaa caatgagtca cgaaataaga acaccaataa gctccattat gggcttcctg
    15481 gaacttctgt cgggttctgg tcttagcaag gagcaacggg tggaggcgat ttcacttgcc
    15541 tacgccaccg gacaatcact cctcggctta attggtgaaa tccttgatgt cgacaaaatt
    15601 gaatcgggta actatcaact tcaaccacaa tgggtcgata tccctacttt agtccagaac
    15661 acttgtcatt ctttcggtgc gattgctgca agcaaatcca tcgcattaag ttgcagcagt
    15721 acgtttccag aacattacca ggttaagatc gaccctcagg cgtttaagca ggtcttatct
    15781 aatttactga gcaatgctct taaatttact accgaggggg cagtaaaaat tacgacctcc
    15841 ctgggtgaca ttgatgacaa ccacgctgtt atcaaaatga cgattatgga ttctggaagt
    15901 ggattatcgc aggaagaaca acaacaactg tttaaacgct atagccaaac aagtgcaggt
    15961 cgtcagcaaa caggttctgg tttaggctta atgatctgca aagaattaat taaaaacatg
    16021 cagggcgatt tgtcattaga aagtcatcca ggcataggaa cgacatttac gatcacaatc
    16081 ccggtagaaa ttacccaaca agtggcggct gtcgaggcaa aagcagaaca acccatgaca
    16141 ctccctgaaa agttgagcat attaatcgcg gatgatcatc cgaccaacag gctattactc
    16201 aaacgccagc taaacctatt aggatatgat gttgatgaag caactgatgg tgtgcaagcg
    16261 ctacacaaag tcagtatgca acattatgat ctgcttatta ctgacgttaa tatgccgaat
    16321 atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt acccatctgg
    16381 gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag ggttaaattg cggcatgaac
    16441 ttatgtttgt tcaaaccgtt gaccctggat gtactgaaaa cacatttaag tcagttacac
    16501 caggttgcgc ctattgcacc tcagtatcgc caccttgata tcgaggccct gaaaaataat
    16561 acggcgaacg atctacaact gatgcaggag attctcatga ctttccagca tgaaacgcat
    16621 aaagatctac ccgctgcgtt tcatgcacta gaagctggcg acaacagaac tttccatcag
    16681 tgtattcatc gcatccacgg tgcggctaac atcctcaatt tgcaaaagtt gattaatatt
    16741 agccatcagt tagaaataac acctgtttca gatgacagta agcctgaaat tcttaagttg
    16801 ctgaactctg ttaaagaaca cattgcagag ctggaccagg agatagctgt tttctgtcaa
    16861 caaaatgact aaatagcggc tcccacaata ttcaaatgtg ggagctattt accagcacat
    16921 cttacattta tgatgaaaac tcctggcgaa tttgttcccc atgttggtcg agcgttgccg
    16981 ctcccggcat aacatgcggg tccgcgcagc cgctgatttt tatcggatta cctggcatca
    17041 ttattccccc ggcttcaatc aacatatttc tcgcctgagt ttgtggcaaa ttaatggcct
    17101 cagccacact taataacggc gcgacgggca caccaacttc atgtattttg gctaaccaaa
    17161 cttcagctgc ctgcgttttt aacgttcgct caatatattg tttaagaata gcctggtttt
    17221 gtacgcgtaa aatattgctg ctaaatcggg gatcattaac cagttccgta agctccagtg
    17281 cctggcataa cgcagaaaaa agcttgtcat taccacaaca aatcgtaatc ggcttatcct
    17341 gagtatcaaa aacatcaaaa ggtgccatgt agggatggcg atttcccagg cgttgtggtg
    17401 acttccctgt cgcaatatat gccatcagac catgctccag aaaactcagc gtggcatcaa
    17461 acatcgctat atcgacatgc gcccctctct ggctcttttc gcggccataa agtgcactca
    17521 ctattccgct gaataaatag acaccgccac ataaatccgc aagagaggta cccacgcgca
    17581 ctggcggagc atcagggtac cctgtttcca tcattatccc gctcattgcc tgaatgatgg
    17641 tatcgtaggc tggagcatct tttagcgggc cggtatgccc aaaacctgac gatgaagcat
    17701 atatgagacg cgggttgatt tcttgtagcg tttcccatga aaaccccagt ttttccattg
    17761 tacctgggcg aaaattctca gctaatacat cagcttgttt aagcatattt ataaatatac
    17821 ttttatcgtg atcattcttt aaatcaagaa ccacactctc tttgccatga ttaataaaac
    17881 tgtaatagag tgactgtcca tccacatagg gaccaaatgt gcgggtatca tcaccatgac
    17941 ccggcggctc aactttaatt acccttgcgc ccatattaca aagaagttga gttccgaaag
    18001 gtccattaag gacatgtgtc atatcgataa ctaataagcc ttcaaacggc cctttgcatt
    18061 cattatttgt catttgctta tgccttgaaa aaagccgaag gtgaaaagac acttcggcat
    18121 ttatgtaaaa tcaataacat gattaattat gaaaccagtc gactgacata aatccacaaa
    18181 ggagccgtga cgacaaagcc cagtacgctc acagccagtg acgctgtacc agtgcgggtg
    18241 tagacattaa accggctggc aataatgatt ccggagaatg ccggcggtaa ggcacctgcc
    18301 agtaccatca tctgcaaatg ttcgctgttc aaatgacatg ccatacccac gagaagcagc
    18361 gccagtggca tcagaatcag cttcaggaag gtgttataag caatttcggc actgaattcg
    18421 aatttatgtg cagccagagt caaccccgca gcgaataccg ctacccctga gttagccttc
    18481 gcaatcagat taaaggttgg gtcccatgct gccggaattt ttaccccaac caacaccagg
    18541 atcgttgcca gaacaggtgc ccatactact ggctcctttg ccgcagaaat taatgcgctc
    18601 agattactat tcttcttacc atccgctcct gaggaaggat tcagcaaata cagaccaata
    18661 ggaatagtaa ttgcgttaac aataatagaa ataattgcta caactaaacc tgttgatacg
    18721 gaatcgccat aaataggatc gagaactgca aatcccagga atccgatggt aggtgaacct
    18781 gcaattaatg cacatactgc tgcttctgca tgagtacgtt taaaaaattt gtagcaacca
    18841 aaccaggaga aaaagaaaca tccgacaata accacaagtg atacaagggt caggcgagtg
    18901 tccgcaaaaa tcatttccct gtttgcccgg gtaatagaaa caaatagggc cgcaggaaga
    18961 gcgtagttta ataccagttt attaaatgcc cgggcttgat cttctgaaaa agtttctcgt
    19021 ctgccgctaa aataacccaa cagcatgatt acgataatag gcaataaatc gccaataaaa
    19081 aatgttagca taatagtatt tccgataagg gtcccctcac catcgataat gagttggcaa
    19141 gaggagacga tgtcagtatt taattaccag cgacttgttt tgggttaagt ttggtaatat
    19201 ggccactttc agtacctgct gcagggtcga tgaccacatt aataatggtc ggtttgcgcg
    19261 actgaatacc ggtggttaaa gcatgacgaa gttcatctgt cgtagtgacg ttatagccaa
    19321 cgccacgaaa cgcatccatt aatttgtcat accttgcatg gtgcaataga tctgttggtg
    19381 atggtgcacc agcgccactg agatcaacac cgtctcctct gtagatgccg ccattattaa
    19441 aaataacgat cgtcaccggc aggttatatc gacaaatcgt ttcaatctcc atcccactga
    19501 aaccaaaagc actatcacct tcaatggcga caaccggaga accagaggtc acgctagcac
    19561 cgatggcata gcccataccg atgcccatga caccccaggt gccacaatcc agacgacgac
    19621 gtggtttata catatcaata atatttcgtg cattatccag ggtatttgcc ccttcattaa
    19681 ctaaataaat atcctggttc tcacgcaata catcgcgcac agcactcaat gcattaaagt
    19741 aatttaatgg ttgggtatct gtacttaact tttcatgcat tttttgcgca ttttgctgct
    19801 tgtggatatt taaaatatcg cgccacacca gcggagtcgt aaatgtgttt tgtttcaatt
    19861 ctgccagcat accttgcata ctggatgcaa tatcgccaac gactggcaca gcaatgggac
    19921 ggttgctgtc aatttcctgc ggttcaattt ccagttgaat aaactgtgta tctgccgccc
    19981 atcctttttt accgtgcgcc agtaaccaat tcagtcgtgc accaacaagc atgacaacgt
    20041 cagcatttgc cagggcaaac gaacgcgcag ctgccgcaga aagtggatgc gtatcttcga
    20101 ggatcccttt cgccatagac attggcagga atggaatctg ggcactttca ataaattcac
    20161 gaagctgttc atcagcttgt gaatacgccg cgcctttgcc aaggataatt aatggtcgtt
    20221 cagcttttgc taaaagcgaa attgcgctag tgactgactt cgggcatggc aataatgctg
    20281 gcgacggatt ttcaacttta acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg
    20341 ccaggacatt tgctggcaaa tcaagataaa ctccgccagg gcgacccgat actgagaccc
    20401 ggatagcgcg tgccaatgca atgccaagat cctgcggctg attaacgcga aacgccgctt
    20461 tggcatacgg ttttgccgca ttcatttggt ccagctcttc ataatcacct tgctgtaggt
    20521 cgacgatcgc gcggtcgctg gagccgctaa tcatgatcat cggaaaacca tttaccgttg
    20581 cgttggccaa tgcggtcaaa ccattgagga atcctggcgc agacactgtc aggcagatcc
    20641 ccggtttttg ggtaagaaaa ccgcttgccg cagcggcata gcctgccgac tgctcatgac
    20701 gaaaaccaat ataacgaatg ccttccgcct gggcatggcg cgccatatcc gtcacaggaa
    20761 tacctacaac accataaata gtgtcaatat cattctgttt taatgcttca acgatgatat
    20821 gcataccatc tgtcatttga agttgatctg acatgattaa taacctcgaa atttttataa
    20881 attgggagcc agaagccccc gttaatatca gatggcgtgg ttttgcttca ttgcagcaat
    20941 ttcatcgtcg ctataaccca gctcctgcaa tacagcagcg gtatgttcac ctaatagcgg
    21001 cgcagcttta atatccggcg taaaggcaga gaatttcatt ggacaaccaa ctgtcagata
    21061 ttttccacgc aacggttgtt ccacttcgac gacactgcca ctttggcgca aagagggatc
    21121 gagtgaaatt tctttcatac ttaaaaccgg tgcacaagga atatcaaatt gagtcaaata
    21181 ggccaccgct tcatgtttat caatagtgac agtgtatttt tcgatttcag caaaaatatc
    21241 gaaaatatgt ggctgtcggg catgggctgt actgtatgcc ggatcggtaa tccaatctgg
    21301 tttgccgatg gctttacagg tattttccca gttttgctcc tgaatagtga aataaatata
    21361 ggcgttagga tcggtttccc agcctttaca tttcaggatc cagccaggct gaccgccccc
    21421 ccccgcatta cctccgcggg gaactgcatc accaaatgtg ccattcggat actgcgggta
    21481 ttcttccaga taacccaatt tatcgagacg ctgctggtcg cgtaatttca cgcggcaaag
    21541 gttcaatacg gcatcctgca ttgacatggt gactcgttgc ccacgccccg ttttttcgcg
    21601 atgcagcaaa gcagcaagta aaccgatcag taaatgcatt ccggtgttgc tgtcacccaa
    21661 cgctgcagcg cttaccagcg gcggaccgtc ccaaaaaccc gtagtggatg cagcgccacc
    21721 cgctgcctga gcaacgtttt cataggcttt tacattcaca taaggcgaac actcatcaaa
    21781 ccctttgatc gaaccaaaaa tcagacgtgg attgatttct tgaatatgct cccaggtgaa
    21841 gcccatgtga tcaatggccc caggatgaaa gttctcgact aagatatcag cttcgcggat
    21901 cagcttttcc attacctctt tgccttccgc tgttttggta tttaattcaa tagaacgttt
    21961 gttactgtta agcatggtga agtaaagcgc atcgatatca ggaatatctc gcagctggtg
    22021 acgcgttacg tcaccaacgc cgggacgttc aattttaatg acgtcagcgc caaaccaggc
    22081 cagcatttga gtacaagatg ggccagattg cacaccggtg aaatcgagaa ctttaattcc
    22141 ttgaagtgga gttgacatag caatacctat tagttaatta aatatgtgtt aatttagcgt
    22201 ttatttaaga cataacgcct ctataaataa tccgcaaaat atcgtcgtag ctgcactcga
    22261 tatttttaaa ttcctgtatc aagtactgaa aatcatttca tcgctacttc caattccttt
    22321 ctgcatggta agtagtgagg acgaaaagaa gaatatgata aatattggtt cgcggtaatc
    22381 agagagagcc gcgacatttg tgatgttatt cttacaactg taagaagcgc cggtgattta
    22441 caagtaaggg tatgattacg ctttttaaag cgtaccataa aacataacat aataaatgat
    22501 ttgtgatgac acgggattat tgcggtttac tttgaggaag ataaaatgcg agtttataga
    22561 accgcgagcg ccaaataatt ttatgaccac aatctgtcgt attgtggtca taaaaaattg
    22621 tcggctggag ggagttaaat cctttacctc aatatactgc ctgtcagcgt tagttcacaa
    22681 acagtgcttc gctatcgacg atgattccat cttccgcacc tttaagcgcc aggtttgcct
    22741 cgtagtattg ctttttatca agcagtttct gcgcatcagc aagtgcatta cgagtctgtt
    22801 tgagcggcat cagatattgg ttttccatta cgccaacacc ggccaggcga agctcttcca
    22861 ttgcgccctt tttatcccct ttcgccattt tttcgttggc aatttttatc gcggcttctt
    22921 tttcgggtgt cgctacataa ctctcagaaa ttccgataga tgcattaatg acaatatatt
    22981 ggtcatcatt gacattggtc tttttacctg gcttagcgaa ctttgcccat tcggtactgt
    23041 catctgacaa caaagctgaa gcttcattgg tcagttcttt tgccttttcc ggatcgccat
    23101 gaaacagagc caggcgcgct acctgcacgt cacgcatcgc atataaacct tgttcagaga
    23161 tacgctctgc tgctgccgtt ttctgggttt gcttaacttg ctgagtttgc tgttgtgccg
    23221 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaactgct gtgaccatcg
    23281 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    23341 atcatattgg gtgagcatga aatgagaaat cagagagcaa cgctcatttt gaaagggtta
    23401 tcttacagtt gtagttatta tcgcaagaag tattttagtc ttacaattgt aggaatatgc
    23461 ttcctgtcac agagaactat tcttatatac cacaaagaga tgtcatttat tatatcgatg
    23521 taaatactga ttaattaaat gtaaatacag ccataacatc gtaggttctc agtgaaccat
    23581 tttcaggata actttctatg aaagtaaact taatgctttt tagcttattt ttattggtct
    23641 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtggaagt gagacgagct
    23701 ataaagagac agaaaaaacg tcaatgatga cgacaactca ttctacaaaa cttcagccat
    23761 cacaggcgat tttgtttaag atgagagaag atgcgccacc attaaatctc acagaagaca
    23821 ttacgccccc ttttccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    23881 cacatggcag gtgaacaatc attcctgatg ctgatgtgag cacctgccca aatgatccat
    23941 cctgaactac taccgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    24001 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    24061 gtagtaaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgagatcac
    24121 gttcagtacc tgaccgatgt atccgcttac gcagacaata cacagggcga gaaacatcca
    24181 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    24241 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    24301 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    24361 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    24421 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    24481 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    24541 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    24601 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    24661 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacggcgtg aatctatcgc
    24721 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgtg agaagatgat
    24781 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg cgtggttctg
    24841 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    24901 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    24961 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    25021 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    25081 aaataatctg cgcggcattg tcggtgcact gaagaaaagt gaagcggtat ggtttgctcc
    25141 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    25201 tgccacaacc aatggtacgt atgtcctttc acgtctctct ggcgcagcca tgttaaccgt
    25261 aacgatggta agaaaagcgg attacagcgg atatcgtttg tacatcaccc cagagatgga
    25321 aggctacccg gcagatgaaa atcaagccgc tgcctatatg aacaagatta tcgaaaaaga
    25381 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    25441 gggagaatcg tcgctataca tttaacaaac tgctcttact ctttccctcc tcaaatcagc
    25501 cataataacc tcatggtttt tatggctttt ttcttaaggt tttttccttc gctgaatctc
    25561 taattattac cgtcagaaat taaactgtcg ccgatatgag acacctgtaa gttactaaaa
    25621 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctgg
    25681 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacaggac ttttaaggtc
    25741 gttttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    25801 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    25861 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    25921 ccatctggct cctgtttgca tttctggcga tggtgtttat tccgcgtttt cgtgaatatg
    25981 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc cacgaatcgc
    26041 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcgc acatgcgtgt cgccgtagtc
    26101 gccaaagccg atccccggag agacacaaac cttcgcttcg ttcagcagct tcttggcaaa
    26161 ttccagcgag cccatggcag catacggttc cgggattttc gcccagacat acatcgaagc
    26221 cttcggcatt tcgaccatcc agcccgcttc atgcagccct tttaccagta catcacggcg
    26281 gcgtttgtac tgttcagcaa tgtcgcgcac gcactgttga tcgccctcca gcgccgcaat
    26341 cgctgccacc tgcaacggcg taaaggtgcc gtaatcgtga tagcttttaa tacgtgccag
    26401 cgcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    26461 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcatgat
    26521 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    26581 cacatcgtag cgtttcgcca gcgccacaac cttttcgaaa aactccagct ccacacattg
    26641 cgcggttgga ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcgcg
    26701 aatggcgcgt tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    26761 ctgcgccccg gcaatcaccg cgccgtaaat atgaatcggg tagcttggat taggcaccag
    26821 tacagtgtca ccatgatcca gcgtcgccag catcagatgc gccagtccct ctttcgaacc
    26881 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    26941 gtgtgaaatg gcgcgacgta gccgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    27001 cgggcgctgg gccaccgtac ataatttttc gacgatgtgc ggcggagtcg caccgtccgg
    27061 gttacccata ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    27121 agcggtaata ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    27181 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    27241 tgccacgatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    27301 gaacattttt atcaatggct ttaaatgagg aacaccactt caaaaacagg aaatttatct
    27361 gcgctaacaa gaaaattacc actcatttta ttaacattat aatttcaata aatttaccaa
    27421 gcagatgtgc attatcaaga gatgcattgc taatacacca tttatctttc aatccccttt
    27481 taaaattgtg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    27541 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    27601 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    27661 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gagctgctcg ctgttaccgc
    27721 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    27781 agtgaacgtt cgtattatcg cggtgatgtc aggcgggatt ctgtttggcc cgtgggtagg
    27841 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    27901 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcgggct ggatcaacct
    27961 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tatgtgaaac
    28021 gttgaccatg attctggtga ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    28081 ctctaaaatc gggattccga tgatcctcgg tagcgtctgt atcggcttta ttgtgctgct
    28141 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    28201 ggatatcgcc aacaaaacgc tgccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    28261 agtttgtgaa attatccgcg atgatattca tgccgatgcg gtggcgatta ccaataccga
    28321 tcatgtgctg gcctatgttg gcgtgggtga acacaactat cagaatggcg atgacttcat
    28381 tagcccgact acccgacagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    28441 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggcgt
    28501 tgtgaccgga acactgaaaa tttactactg ccacgcgcat cagattacgt catcattaca
    28561 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    28621 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    28681 ccatttcctg tttaacgctt tgaacgctat ttcatcgtca atccgtctga atccggatac
    28741 cgctcgccag ctgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    28801 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    28861 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    28921 ctgcattccc agcctgctga tccagccgtt ggtggaaaac gccattgtcc acggtatcca
    28981 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggta atcgggtacg
    29041 cattgcggtg cgagacaccg ggcacggcat cgatccaaaa gtcattgagc gggtcgaagc
    29101 gaacgaaatg ccgggcaata aaattggtct gctgaatgtc catcatcgcg tgaagttatt
    29161 gtatggcgag gggctgcata tccgccgcct ggaaccagga acggaaattg cgttttacat
    29221 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    29281 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    29341 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    29401 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    29461 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttt
    29521 atcaccgcat ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    29581 ctcaaaccgt atcaggagtc acgcattacc gggatgctgc aaaaactgga agcagcctgg
    29641 caacaacagc agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa tgacaccatt
    29701 aatctggtga aagatgagcg aatcattgtc acgccaatta acgatatcta ttacgccgaa
    29761 gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat cctacgtaat gccgatgaac
    29821 attaccgagt tttgcagcaa gctgccgccg tcgcattttt tccgctgcca tcgctcattt
    29881 tgtgtcaatc tgaacaaaat ccgcgagatc gaaccgtggt ttaataacac ctatattctg
    29941 cggctgaaag atctggattt tgaagtgccg gtcagccgca gcaaagttaa agaattccgc
    30001 cagttaatgc atctttaatc gggaactttc atgaaagcac caggcttgcc cgccgatcaa
    30061 caatttttcg ccgatctgtt cagcggcctg gtgcttaacc cgcaactact ggggcgcgtc
    30121 tggtttgcca gccagcctgc ctcattgcca gtgggcagtt tatgtattga ttttccccgt
    30181 ctggatatcg tgctacgcgg cgaatacggc aatctgctgg aagcaaagca gcaacgtatg
    30241 gtggaaggag aaatgctgtt tattccggca cgcgcggcta atttaccggt caacaacaaa
    30301 ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc ttggcttatc attttacgat
    30361 agccgcacca cgtcgttgtt acatcctgct cgccagatcc aacttcccag cctgcaacgc
    30421 ggcgaaggtg aagcaatgct tactgccctc acccatctta gccgttcgcc gctggagcag
    30481 aatatcattc agccgctggt gttaagtttg ctgcatcttt gccgtaacgt ggtgaatatg
    30541 ccgccgggca attcgcagcc gcgcggcgat tttctctatc acagcatttg taactgggtt
    30601 caggataatt atgcccagcc gctcacccgt gagagcgtgg cgcagttttt taatatcacg
    30661 cccaatcatc tgtcaaaact gtttgcgcag catggaacaa tgcgttttat cgaatatgtg
    30721 cgttgggtgc gaatggcgaa ggcgaggatg attttgcaga aatatcatct gtcgattcat
    30781 gaagtggcac agcgttgcgg ttttccggac agcgactatt tttgtcgcgt tttccggcgt
    30841 cagtttggcc tgacgccggg agagtacagc gcccgttttc agggctaacg tcagaaggtt
    30901 aattccgttt ccagcagcgt caggatgctt tgcgcatctt gcgcggcgaa cagcgactgg
    30961 cggaagtttt tattcaccag tttacgcgcc agctgcgaga agactttcac atgattcatc
    31021 ccttcgttag cgcccagtgt cagcatgatc accagttcga cttcgcccat ttctgactgc
    31081 cagtcaaccg gtttcaccag ccgggcaatg ctgatactgg aatgacggat ccactgagat
    31141 ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg taacaatctc ttcccgctgc
    31201 cagacatcct cttccagctc gaacggatgt tcagtgcgcc cgttaacgcc gaggttaccg
    31261 cacaggaact ggatcgcctg ctctttattg ctaaaggact gatcaacaaa gatattctcc
    31321 agcgccagca gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa cgcttcaatt
    31381 tcctgcgcgc tgcggcattc acatgcctga cgcgccagtt cccgacacgc ctcgctatcc
    31441 agttgacgaa gctggctttt caccgccgga atgcgcgggc tactcatact taactcgtcc
    31501 aggcccagcc caagcagtag cggcagataa cggctttcac cgcccagttc accgcaaatg
    31561 cctacccatt taccccgctg atgcgcagtg gtaataatct gctgcaacat gcgcaggaac
    31621 gatggcgtaa tcgggttata cagcggcgat acgcgcgggt tattacgatc gaccgcatac
    31681 agatactggg tcatatcgtt ggagccgata ctgaagaaat ctacctcatc gcagaagtga
    31741 tcgatgatgt agcacaccga cggcacttcc accatgatac ccagcgtaat cgtctctgca
    31801 tgacgcaggc catcgcgctt aagctcaacg atcgcttttt ggatctcgcc cttcacccat
    31861 aagatctgat cgaggctgtg aaccattggg atcatcaact gggcgttgcc gaaactggcg
    31921 gcgcgcaaaa tggcccgcag ttgagtgcgg aacaggcccg caaattccgg gtaaatacgt
    31981 accgcgcgat agccgaggaa cgggttctct tcctggggaa tattcagata aggaatgctt
    32041 ttatcgccgc cgatgtccat cgtgcggaag ataatcggct tgtcgcccgc cgccagtagc
    32101 acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcgcg atccatatag
    32161 agcatttcag tacggaacag cccaacacct tccgcgccgt tggcaaacgc gcctggcgct
    32221 tccagagcgg tgccgatatt cgccgcaata tcaatacgct tgttatcacg ggaataggcc
    32281 agctgcgcgg cggcctgcgc ctgttgtttt tggcgtttat ccgccagcgt ctgcgcgacc
    32341 tgataataac cgctcaccgc gtcatcaggg ttaatcgcca gcacgccgca ctgggcgtca
    32401 agcactgccg gttgcccggc atagcgcgcg atcgcatcca gcggcaagcc actcagtacc
    32461 gggatcaccg aggcacgggc cagaatcagg gtatgcgagg tgcgcccggt tttctccaga
    32521 atcatgcccg caagattttt taaatcgagg ctcaaaaact ggcttggggt taaatcctcg
    32581 gccaccagaa tagtcggttt ttccagcacc agattgttgc gcggcttcag ttccggccag
    32641 gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc tgacccgctc gcgcagataa
    32701 tcgctggcag aagcagatag tttggcgcaa acctgctcca tattgctgat gatcgccgcc
    32761 cccagcccct gatgctgttc tgccataagg cgacggatat tgcctgcaaa ttcatcatcc
    32821 tgaatcagcg acaaatgggc gctgaggata gttttgcttt cgccgtcacg ctcacgcagc
    32881 tgctggttca gttgctcggc aagcgttgcc aggctgtgct ccagccgggt ggaatcttgc
    32941 gcactggcgg ggatcgcccg gtaactgtcg aggctgtcgc tctgtaacag gatcagcgta
    33001 cccacgccga cgccgcttgc cagcacattg ccgtacaata aatcaggatt cagacggctt
    33061 aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg ttggctggac gctgtcgctg
    33121 tcgataaagc gcacctggat gtactcttcc agcacgcgcc gcgcctgctc ttcatcgctg
    33181 ccgctaatgt tcaggctgca actgtcatta aataaagtgc cggtaccaat cagcgccagc
    33241 gaacttttcg catcggcctt tgcgttctgg cgatggttaa taaaagtgat ttcgctttgc
    33301 cactggctgc actgttcttt aagttcccac gccggacgag catgaatacc gttaggcaaa
    33361 ggacagagaa attgaatcgt taacataacc gctccgggtt aatcaggaga tcatctgaaa
    33421 tccgtcagtt gaacaaccgt ctcacgggta agacgttgaa taagggcaga taaaagttgc
    33481 tgcatctgca aaatgtcgcg gcaatcggca atcgatgccg cacaatgtcc gtggcgcgtc
    33541 gctggcccca tcaccaccgt gggtacgccg gtgtcagtta aatgcaccgc tccaccgtcc
    33601 gtgccgccgt tgctgaacat atctgcctgt aacggcacgc caatttctgc cgccacggtt
    33661 tcaatccacg cggtaagttt tggcggcgca atcagcgact tgtcgcttaa taccagcatc
    33721 gggccgttac caatctggcg atggttagcc gcgccataat caaagttttt cgcccagcag
    33781 gcggtatcca gcacaatcgc aacgtccggt gaaaccgcac gggtagcggt ttgcccgccg
    33841 cgtaatccca cctcttcgct ggagctggcc accagccaca cctccgcagg cagttcggcg
    33901 tcgtgcagct cacgtagtaa cgtcaccaac agatagcaac cgaggcgatc atcaaaggct
    33961 ttccccatca cccgctggtg agggagaacc tgaaaagttg tatcaaaggt gacgcgatcg
    34021 cctggacgaa ttcccgcctg catcacttcg tcatacgagc gcgcaccaat gtccacgcgc
    34081 atggcgctga cgtcattccc ctgccggtcg ccgtcaagca ggcctggaat tttgcactct
    34141 tcacgggtag tgatacgcac cgactgaagc tggcgggcag ccatgcgtac attgccaacc
    34201 ggcaacacat cgatcgcccc ttcgcgggag atgctgcgca ccataaatcc cacttcatcc
    34261 atatgcgcac agatcatcac cttcggacct gtcgattcat tgaggcggat cagcaccgat
    34321 cccaggccgt caaatcgcac ttctttttgc aggcgatccg cttcttccag caggatctgc
    34381 cgcacttcct gttccgagga ggcgatcgca tctgcctcgc tcaacgcttt taataacgat
    34441 aaatccatta tgcctctccc gtgagcaaca ctgttttcgg catggcgtag agcacttctg
    34501 cgccttgcgg ggtgaccagc acaacatcct cgatgcgcac gcccccttgc cctggcaaat
    34561 aaatccccgg ctccacggtc agtaacatgc ctggctgtag cgtcgtggtg tcccgcggtg
    34621 aaaaacgcgg atcctcatgg acttcaatac cgatagcatg accggtgtta tgaccgaaat
    34681 tgtcaccaaa tccagcctcg gtaatcaccc ggcgcgcggc gtcgtcaacc tgctggcagc
    34741 gcacgccggg gcgaatcgcg gagattgctg cgagctgtgc ctgcaggaca atttgataga
    34801 cgttaaacag cgggtgagat tcggcgctca ccccttcgcc attcaccagc aaggtgcgcg
    34861 tcatatcaga gcagtacccc tgatagagcg caccgaaatc gagagtgaca aactcgcccg
    34921 ctgtaacaat cttgtcgctg gctttgccgt gcggcagcgc cccacgccag ccgctggcga
    34981 caatggtgtc aaaagaggct ttttctgcgc cctgctggcg cataaaccac tccagttcag
    35041 cggctatctc gcactcgctc attcccgcct gaataaagcg gcgaatatgc tctgcaccgc
    35101 gatcggcaat cccacaggcg aggcggattt tctccacctc ctctggcgtt ttgatttgcc
    35161 gcagcacatc cggcgtggcg cttaccagtt tcgcattgag ttcagactgc cagcggtgcg
    35221 cggtttccca actcacctgc tggccctcaa aaccgagcgt ttgcaactgc tcatcggcaa
    35281 tgatttgcct ggcgatggtt gtaagcttgt gcgtcgcgtc aagcaaatgc agctggtaac
    35341 cttgcgtgcg ggcttctaca tccgcgtaat agcgcgagtc caccagaatg tgcgcacttt
    35401 cacggctaat caccacatag cctgatccgg tggagatccc cagatgcggc tgtttgttct
    35461 gccgtgagga gagaagcacc gcatccagtt gttgcgcctt aagccagtcg cgcagcgaag
    35521 cgagtaatgt catcaggatt ttccttctta caggctatcg attaacaatt tgcctttacg
    35581 gaacatcatc agacgcagga acaccaccat cagcgcagta atgactgctc ccagcgcgat
    35641 ccccgccatg tagacgccga ggttagtgac cagtggccat gcccagatag cggattccgg
    35701 gaaccattgc actgcgccca gccagactgc agcggttgag ccgacaatcg cgccaaccat
    35761 ataggacgga atggcggtga tggggctttc cagcgcaaac ggaatcgcac cttcactgat
    35821 ccccataaag gcaaggaaca tggcggtttt accctgcgga taaagctgag cgttgaacag
    35881 gcgtttgccg gttaaacgac ggtcaataat ggtcgccaga cccagaccaa tcggcggaat
    35941 aacgatagcg atagaacgtg cggtgaccgg taatacgtgg tccgtggtaa agctgaaggc
    36001 aacgaaacct gcggctttgt tgatcgggcc accaaggtcg attgctgtcg ccgcggcgat
    36061 ccccatcgcg tacatcagcg cgcccttctc acctgcagcg gtcagcacgg tacggatacc
    36121 gccgttgatc cagccaccaa agggggtgat gacgtagtac atcgccagca tgacaaaaat
    36181 ggcggaaaga atcggtagca aaaatgtggt tttgaacgct aacaggaaat ccggtaactg
    36241 gattttctgg ttcatccatt tcacgaggta acccgcaacg atagatatta tcagcgcacc
    36301 aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc atagtgctgg gatcgaagtt
    36361 cagcagttgc gtcggctggg tagacatcaa gccgccgata aaacccgccg ggaacgccag
    36421 tttgccaccg atagagtttg ccacaaaagc agcaaacatc ggaatggcaa aaccaaacaa
    36481 cacgccgccg aaggactgcg acagccaggc aaatttaagt aaggagaggt cgaaaccgga
    36541 gaatttcccg ctattaagag catccatgat gccgatatca gcaggaattt taagccagct
    36601 ataagcaatc agctggctga aagcgaggat cacaccgccc ataattaagg tcggcaccat
    36661 gcgtgaaatc cctgacatca catgctgcgg gagttcaccc cagaagctgg ttttagaggc
    36721 ctgcggattc ttaattgccg ctgccgcacc ggatgcgcca ggcacaacgg ttgcactgcg
    36781 ttttttaatg gccataacaa ttatccctgt cgaatattac tgttgttcag aggcaatcat
    36841 ctcttcgatt tctttgatga tgcccgcagc gtttttaatt gcgtcctgca aagtgatttc
    36901 ataaacgtcg cgtgattcga aacgttcgtt atcttccggg gtaaccgcca cggagtggat
    36961 gatgatggtc gcttcggcga tatcctgcgc ggtcagacga ttctggatac cgtccgcacc
    37021 ctgggtttca attttcactt cataaccggc ttctaccgcc gcttcttcca gcgcctgagc
    37081 ggccataaag gtgtgagcca ggcccattgg gcaggcacat aaggcaatca gtttcttact
    37141 catcgttaat tcctcgttca gtgatagttg acgagtgaaa ttatgaacgt gcaaaacaaa
    37201 tcgccgttac cagacaaata atgcatttac tggagaatta atcccgctgc acggaagtgt
    37261 gacgcaggtc ggccttgtga ggggacacaa aggaaaattg aattaactgt atgatttaaa
    37321 agattatcgg gagagttacc tcccgatata acaggaagga gttacagaat gtggcctaag
    37381 gtctggcgta aatgtgcgcc ggaaccgaga aggcccggat tgtcatggac gatgagatac
    37441 accggaatat catggacata ttctttaaag cgccctttat cttcaaatgc ggcacggaaa
    37501 ccggaggctt tgaagaactc aaggaagcgc ggcacgatac cgcccgcaat aaacacgccg
    37561 ccaaatgtcc cgagattgag cgccagattg ccgccaaaac ggcccataat gacgcaaaac
    37621 agcgacaacg cgcggcggca atcggtgcag ctgtcagcca gcgcgcgttc ggtaatatct
    37681 tttggcttga gattttctgg caggcggttg tcggctttca caattgcgcg atacaaattc
    37741 accagcccag ggccagaaag cacacgttcc gccgaaacat ggccaatttc cgcacgcaat
    37801 atttcgagga taatggcctc ttcttcacta ttcggcgcaa aatcaacgtg accgccttcg
    37861 cctggcaagc ttatccaacg cttatcgaca tggaccagat gcgcaacccc tagccccgtt
    37921 ccggcaccgt aaaccgcaat aggcttacct tctaccggtt ctgcgccacc aaactgaatc
    37981 agatgctctt ttttcagcat cgggatcgcc atcgatacag cggtaaaatc gttaataatt
    38041 tccagatggc taaaaccgag attctttttc atttcggcaa ttgagaacgc ccaggtatgg
    38101 ttggtcattg ccacccagtc accggtaatt gggcaagcga tggcaataca gccgtctttc
    38161 acctcgacct tatgttcttc aagataaacg cgaatgaccg cttcgaggct ggggtaatca
    38221 agccctgaat aggtcttagc ctgcgagatt tcaccactgg caatatcaca cagagcaaga
    38281 cgtgcgttgg tgccgcccac atcaccgact aatgcatact ttgtcattct tcaactgctc
    38341 cgctaaagtc aaaataattc tttctcacac tgtaaatacc tgggggcata acaacaacgc
    38401 cggaaaggca ggctccctgt aaatatcgat ctgggtcaca caattacttt atcgtttcag
    38461 caccaattgc agcgatgcct ttttgcaagc tgggcaaact aagtatctga ccccgcataa
    38521 ggaatagaat atgctccatc cgcgagccag aaccatgttg ttattatcgc tccccgccgt
    38581 ggcaattggg attgcgtcca gtcttattct gattgtggtg atgaaaatcg cctcggtatt
    38641 acagaatttg ctctggcaac gactgccggg aactctgggg atagcccagg attcaccttt
    38701 ctggatcatc gccatattaa cgctaacagg tattgcggtg gggttggtta tccgtttcag
    38761 ccaggggcat gccggaccag accccgcctg tgaaccgctg atcggcgcac cagttccgcc
    38821 ctctgcgcta ccaggactta tcgtagcatt aattcttggt cttgctggtg gcgtcagcct
    38881 ggggccagaa catccgatca tgaccgtcaa tatcgccctt gcggttgcca ttggcgctcg
    38941 tctgttaccg cgtgtcaacc gaatggagtg gactatttta gcctctgccg gaaccatcgg
    39001 cgcgctgttt ggcacacctg ttgcggcggc gttgatattt tcgcaaacct taaatggtag
    39061 tagtgaagtt ccgctatggg atcgtctttt tgcgccgtta atggcggcag cagctggtgc
    39121 acttactacc ggattatttt tccatcccca tttttcactg cccattgcgc attacggaca
    39181 aatggaaatg accgatattc tcagcggtgc aattgtcgcg gcgattgcca tcgcagcagg
    39241 gatggtcgcc gtatggtgct taccacggtt gcacgcgatg atgcatcaaa tgaaaaatcc
    39301 ggtgctcgtg ctgggtattg gcggatttat tctcggtatt ctgggggtta tgggtggacc
    39361 agtttctctg tttaaagggc tggatgagat gcagcagatg gtggcaaatc aggctttcag
    39421 caccagcgat tactttttgc tggcggtaat taaacttgcc gccctggtcg ttgctgccgc
    39481 cagtggcttt cgcggtgggc gaatcttccc ggcagtgttt gtcggcgtgg cattagggtt
    39541 gatgctgcat gagcacgtcc ccgccgttcc agcggcaata accgtttcct gcgctattct
    39601 cggcatcgtg ctggtggtaa cacgcgatgg ctggttaagt ctttttatgg cggcagtcgt
    39661 tgtacccaat accacattgc taccgctgct ctgtatcgtc atgcttccgg catggctgtt
    39721 attagcaggt aagccgatga tgatggtcaa tcgtccgaag caacagccac cccacgataa
    39781 cgtttagcaa aaaatgctct cctttatgat ttaagagtta tggcgatgat acggggcttt
    39841 acctccccgt aatattgcgt taacaggccg ctgacggctt cgatcgtgaa ggagaataac
    39901 aatgttcagg tcactgtttc tggcggccgc cctgatggca tttaccccgc ttgcggcaaa
    39961 cgcaggtgaa atcaccctac tgccatcaat caaattacaa attggcgatc gcgatcatta
    40021 cggtaattac tgggacggtg gtcactggcg cgaccgtgac tactggcatc gcaattatga
    40081 atggcgtaag aaccgctggt ggcgtcatga taatggctac catcgtggct gggataagcg
    40141 taaagcgtat gagcgtggct atcgtgaagg ctggcgcgat cgtgacgatc atcgcggaaa
    40201 aggccgcgga catggacacc gccattaaac acattcgcca atggagcaca acgcctgatg
    40261 cgacgctagc gcgtcttgtc aggcctacaa accgtctctt taacgcgtag ggcggataag
    40321 gcgttttacg ccgcatccgc cagtgatgac tggtacgacg ccagttgaac tatagcccca
    40381 gcgccgtccc caccaacaac cagatattca gcgccactac cagcaccaca atcacccagc
    40441 ctgtctgttt tacgcgttta ctgttcacca gatcgcccat caacttactg tcactggtaa
    40501 aaatcagcag tggaaccagc gccagagcga taccaaaact taacagcacc tgactcataa
    40561 ccagaatccg tgtcggatct aatcccatca gaatgacaat aaatgacggc agcatggtga
    40621 ctgtacgacg cacccacagc gggatatgaa agcgaatgaa gccctgcatc accacctgcc
    40681 ccgccagtgt ccccaccacc gttgaggaca gtccggcagc aaccaggctt aatccaaaga
    40741 ccgttgccgc agcatggctt aacaacggtt gcagcgtcag gtaagcctca tcaagatcgg
    40801 caacaccagt atgacctgaa aaatggaacg ccgccgcagc tgtagccatc atcgccagat
    40861 tgacaaaacc ggcaatagtc atggcgatag ccacatccca tttggtggcg gaataacgtt
    40921 gttgacgaga accgccatgt aaatgttgag tgagcgaaga gtgcaaataa atcacatgcg
    40981 gcataatcgt cgcccctaac acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac
    41041 tcgggatcac cattccttta cccagctgcg ccaggttagg ctgggagaaa atcaactcga
    41101 caatgtaagc cgcggcaaca aacaacagta acccgccaat cactttctcc agtggttttt
    41161 gcccgcgacg ttgcagcatt aaaatcagga aagtcgcgat ccccgtcagc accgcgcctt
    41221 gcaacaacga aacaccaaga atgagtttaa aaccgatcgc cgcaccaata aattccgcca
    41281 ggtcggttgc catcgcaata atttctgcct gaacccaata gaaccacact acgggacgcg
    41341 gatagtgatc acgaatctgc tccgccagat ttttaccggt ggcaatcccc agtttggcag
    41401 agaggatctg aatcagcatc gccatcaggt tggcccaaac gacaacccac agtagctgat
    41461 agccgaagct ggcacccgcc tgaatattgg tcgcaaaatt accgggatcg atataaccaa
    41521 tcgccgcaat gaacgcaggt cccattaatg cgagcctcat cttgcgcgcc gcccgtccgc
    41581 tgctactctc aacgcgatag ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt
    41641 ttcatgctat gccaaacgag aatgattatc aaattcattt aaatgggttg tggtgatttc
    41701 tctgatagac caggattatg actacgaaaa gattgatgac ttgaatgtta tggttatgtt
    41761 taatgttagc acatttacat aactttcagc ttccatacac aacatagcag aaatgtatga
    41821 cagctcacta tttttgaagc ttgtcacagg acgtcattat agtgtgtgtc agatctcgtt
    41881 ttccttaacc atgttacata gaatgtgcac ggaaatttaa cctgcctcat atttggagca
    41941 aatatggacc gcgtccttca ttttgtactg gcacttgccg ttgttgcgat tctcgcactg
    42001 ctggtaagca gcgaccgcaa aaaaattcgt atccgttatg ttattcaact gcttgttatc
    42061 gaagtgttac tggcgtggtt cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc
    42121 ttctccgaaa tgttcgaaaa actgctcgga tttgccaacg aagggactaa cttcgtcttt
    42181 ggtagcatga atgatcaagg cctggcattc ttcttcctga aagtgctgtg cccaatcgtc
    42241 tttatctctg cattgatcgg tattctccag cacattcgcg tgttgccggt gattatccgc
    42301 gcaattggtt tcctgctctc caaagtcaac ggcatgggca aactggaatc ctttaacgcc
    42361 gtcagctccc tgattctggg tcagtctgaa aactttattg cctataaaga catcctcggc
    42421 aaaatctccc gcaatcgtat gtacaccatg gcagcaacgg cgatgtccac cgtgtcgatg
    42481 tccatcgttg gtgcatacat gaccatgctg gaaccgaaat acgtcgttgc ggcactggta
    42541 ctgaacatgt tcagcacctt tatcgtgctg tcgctgatca acccttaccg tgttgatgcc
    42601 agtgaagaaa acatccagat gtccaacctg cacgaaggcc agagcttctt cgaaatgctg
    42661 ggtgaataca ttctggcagg tttcaaagtc gccattatcg ttgccgcgat gctgatcggc
    42721 tttatcgccc tgatcgccgc gctgaacgca ctgtttgcca ccgtgactgg ctggtttggc
    42781 tacagcatct ccttccaggg catcctgggc tacatcttct atccgattgc atgggtgatg
    42841 ggtgttcctt ccagtgaagc actgcaagtg ggcagtatca tggcgaccaa actggtttcc
    42901 aacgagttcg ttgcgatgat ggatctgcag aaaattgcct ctacactctc tccgcgtgct
    42961 gaaggcatca tctctgtgtt cctggtttcc ttcgctaact tctcttcaat cgggattatc
    43021 gcaggtgcag ttaaaggcct gaatgaagag caaggtaacg tggtttctcg cttcggtctg
    43081 aagctggttt acggctctac cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg
    43141 ctgtaagacc atacataaaa aagccgggga taattccccg gtttttttta cgcctgttaa
    43201 tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta
    43261 tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc
    43321 gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata
    43381 tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg
    43441 tagttggcat agccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc
    43501 tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca
    43561 agaatgaccg cctgcgggga gatgttataa cgcttaaaca gacgaataat ccgcccggca
    43621 atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg gccttttttg
    43681 tcgcaaggat gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc
    43741 gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg
    43801 tcatatttca gtcgcgcgag gatttcatca taaccttcac cctctttgtt gcgaattggc
    43861 tgggcgtaga gcagcaaatc accctgatct aacgccgtac gaatggtatt cagcaatagc
    43921 acctgtttag tggtctgccc ggaaaccatc tcctctcggc tatccagcgc cagcacatgg
    43981 tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt
    44041 tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtatta
    44101 ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt
    44161 tccggtccac tcagcactaa cagcagttca ctacccggca attgatacaa cttttcgttt
    44221 tcctgcatca acggcagcag cgtacggtaa attgatcgga tacagtgaac gcgcatcatt
    44281 aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaaactcttg
    44341 cccgcctctt gctccggcgc ttgttctaac gcacgaaagt tgggtaggag cgttaacgga
    44401 tcggtcagcg cctgcaaatg ccagcggcga ttaagccatt cactgcgatg ataaatgcgc
    44461 accatataga gcaggcaaac actaaaggaa atcagcaccg cgagaataaa tgccagcgaa
    44521 tattcggtta gtaccccttg caaaaagttc tggttgtaat tcagcaggca aagcgttgaa
    44581 acagcccagg tgagatttaa aaacggatag cgaagcttac cgacaccgag ggtaaagatg
    44641 ataaagaaaa caggtaccag gtaaccggca ataaagtcat tctcatacgg tgtgcacatc
    44701 agaagtagca gcacgctaag agctgccagc cagcttaagg taaatgcgcg tttctctttg
    44761 ctcaacgacg gagcgatatc cctgcgccac aatatctgcg caaagtgggg acttacaatc
    44821 atgcgagtga gatagtagaa aagcatgttg taaatcagca cagcggtgaa aaggcttagt
    44881 aaatcaacga ctgtgaaaat ggcatccgca tcgccgaaaa aggtggatat ctttagcggg
    44941 aaatcaaaaa agctccccac aagatacatg ctgcatttga tgccgatcgg cgtcaccaaa
    45001 ccaagccaga aaagacgttg ccagacatat cggctggtca atccataacg ccaacgcgtc
    45061 cccagctgcc agcgtaatat tgcgcaggca cacaacacaa caaacgtctg gctgccgagc
    45121 atgaccgcgg tctgtaaaaa agatagatga aagttccaca gattggtgca aaacatcccg
    45181 cataaaacgg gaacgacacc gcgccagcca aagataaaca agattgacag cattacgcac
    45241 agtggcatcc acgccagaaa gatataactg gaatggataa tggcaagtgg cgaaataaaa
    45301 cgggatagct gaataagtac cacggtgagc gtaaacgcta gtgtgaatat cttgatattt
    45361 tttatcaggt tatgctccac aaacatgagt acactactct cactgatcgg ggttttcagg
    45421 cacacaaata ataagtttat tcgcatcgcg aagcaaacag gggccagcct gttgccgatt
    45481 tttcatccaa tgatgctttc accaggcggc gctaacccgg acgtagactg tattacaaaa
    45541 gcggcaaaaa gcagagacaa aaaacccccg ctttacagcg agggtttgaa atttggtgga
    45601 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    45661 tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa
    45721 gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag
    45781 ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt
    45841 gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt
    45901 ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta
    45961 taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc aaggagagga
    46021 tgacgccaga tgaacttgcc agactgaccg gctatagccg gcagaccatt aataaatggg
    46081 tacgcaagga aggctggaca acgtcaccaa aacctggcgt ccagggtggc aaagcccgac
    46141 tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc
    46201 agggagaagc gcctgccctg tctggtgatg cgccgcttga agtgttgctg gtgacactgg
    46261 cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga
    46321 ttatcggatt attacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc
    46381 aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac
    46441 cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ctggcgtgaa aggcggtcgt
    46501 gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc
    46561 cataacacac cgatgcttct ggaagccgaa gaaccccttg ccgaatacgc accgggtatt
    46621 cgcacccctg cttatcggca aatcatcagc gcaatagaca acatgactca cagtgagcag
    46681 gaaaaagttg cgcaattttt gtcacgcgaa ggaattcgta atttccttgc tcgtctcgac
    46741 atagatgaat cagaataaat aaaaaacggc aggggtatct ctcctgccgt ttatctttta
    46801 tgcgctaatt actgctgatt ttcgcgttca gcaataaaag ccagcgcttt gttgatacgc
    46861 tcgatactgc gggtcttacc gattgcgtga acggtaacat ccagcgccgg agactgccct
    46921 gcaccggtta cggcgacacg cagcggcata ccaactttac ccatacccac ttccagctca
    46981 tccgccgtcg cctgaatagc gtgatgaacg ttttcagcgg tccagtcagt aatcgcggcc
    47041 agtttgtcac gaaccacttc cagcggctga cgcgctaccg gacgcagatg ttttttcgcg
    47101 gcgtcggcat cgaactcagc aaaatcttcg tagaaataac ggcagctctg tgccatctct
    47161 ttcagcgtct tgcagcgttc acccagtagt ttcaccagat cagccagctg cgggccgtta
    47221 cgggtatcga tattttcctg ctcaatgtgc cactgtaagt gagtagcaac atactccggc
    47281 ggtagcgcgt taatgtagtg atggttcagc catagcagct tgtcggtgtt gaacgcactg
    47341 gcagatttgc tgacggcatt cagggtgaag tatttgatca tctcttcacg agtgaagatt
    47401 tcctgatcgc cgtgggacca gcccagacgc accagatagt tcagcagtgc ttctggcaga
    47461 taaccatcat cacgatactg cattacgctg accgccccgt gacgtttgga cagtttttta
    47521 ccgtcatcgc cgttgatcat agaaacgtgc gcgtaaaccg gtaccggcgc tttcagtgct
    47581 ttaagaatgt tgatctggcg tggcgtgttg ttgatatggt cttcgccacg gataacgtgg
    47641 gtgatttcca tatcccagtc atcgacaacc acacagaagt tataggttgg agaaccatcg
    47701 gtacgacgga taatcagatc atccagctcc tggttgctga attcgatcgg accacggatc
    47761 tgatcgtcaa aaacaacaga accttcctgc gggttagcaa aacgtacaac acacggttca
    47821 tcatcagcat gatgctcatg gctgtggcgg cagcgaccgt cataacgcgg cttctcacct
    47881 ttcgccattt gctcttcgcg cagcgcttcc aggcgctctt tagagcaata gcatttataa
    47941 gcagtgccct cttccagcat ctgatcgatc accgcgttgt agcgatcaaa acgtttggtc
    48001 tggtagtacg gaccttcatc ccactccagg ctcagccagt tcatgccatc cataatggct
    48061 tcgatagctt ccggcgtgga acgctcaaga tcggtgtctt caatacgcag cacgaactca
    48121 ccgccgtggt tacgtgcaaa aagccaggag taaagagcag tacgcgcgcc gccaacgtgc
    48181 agatagcctg ttgggcttgg cgcgaagcga gttttgattt tcatgaaatg gccttacgtt
    48241 tagaaagatg ccgacaaccg gcaaatcctg gaaaaattga gtgggcgata ttctatcact
    48301 cccgcctgat tcctcaatgt agttcgggtt gcaaccttac gcattattgt tttcgttgac
    48361 aaattgcgca acacggtctg ttttgcgatc atcttgctta aatttacgac gaacgaacaa
    48421 tttctttaga aaatgcgttg actcattttg aactctccct ataatgcgac tccacacagc
    48481 gggggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
    48541 tcccgtcatc acccaccaac tactttatgt agtctccgcc gtgtagcaag aaattgagaa
    48601 gtgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
    48661 tcccgtcatc acccaccact tcgggtcgtt agctcagttg gtagagcagt tgacttttaa
    48721 tcaattggtc gcaggttcga atcctgcacg acccaccaat gtaaaaaagc gccctaaagg
    48781 cgcttttttg ctatctgcaa tttgcgaaat tgcctgatgc gcttcgctta tcagaccaac
    48841 tatgtccggc agttcccgtg cctctcatct actgcgccaa cgccgccagc agtttaacca
    48901 gcgcgggcgt cacctgctgc gtttcataaa caatatataa gtctgccggg atgcgttctt
    48961 tgagcggacg gaatatgaca ccgggccagt tcatctgcgc gtagctgtcc gctatcaatg
    49021 tgataccaat acccatactg accatggcga gtaccgtttg cggttcatta acttcgcgaa
    49081 taacaaccgg tgaaaatccc acctgctggc aaactcgctg caaaaaatcc cagtcagtgt
    49141 acacgggcgg cattgtaaca aaatactcgt cacctagcgc ttccagcgga acggtggaaa
    49201 atgatgagag atgatgctct tcaggcatcg ccaccagaaa cgccgattca tgcaaccgta
    49261 agctggtaaa accagccggt ggttctgtcg ccattctcca gattccggca tcgagttcac
    49321 gccgttccag cagggccatt tgcatcgcgg gcatcttttc gcgaaaaaga acgtcaacgt
    49381 taggattttc cctgaggaat cgccgcataa ccgggcgcat ccgtccccac attgccgttc
    49441 ccacaacccc gagttcaatc cgccctgctt ctccccgacc tatttgttca acccgagcca
    49501 atacattatt tgcattcacc agcaatcgac gcgattcttc catcaagatt ttgcccgcgt
    49561 gtgtcagtac gacgctgcgc gaatggcgaa taaaaagctg cgtgccaagt tgattttcca
    49621 gctctttaat atgaatactg agcggaggct gagacatatt taaacgcgct gctgcgcgac
    49681 caaaatgcaa ctcttccgct acggcaagaa aataacggag caacttaaga tctgttctat
    49741 atacgcgttc cataattaag tgctccatat aataaataca tcgtattaaa ttatcatggc
    49801 ataaagtatt tcaccaccag atatccgaca tacataaaca cgatggataa tatacccggc
    49861 aatacatttg gaacaggaat aggtaatcgc acgattgtaa acaaagcacc aatgccaaaa
    49921 ccaacgccag aagaaaataa aatatctttc actaattaac ctttatcata aaagcagccc
    49981 caaggaacag agctgcgaat ccttttaatg tgtaactgaa cgatgcgtta tcttttcagg
    50041 gtcatgatta tatttaaacc caaagaaaaa tatcactgcg agaaaaagag catatcctgc
    50101 aaacaccagc cagatagttt gccagtcttt tacgccatcc accgaaaagt aatcaactgc
    50161 catgccactc agaatcgagc caacccatgc gccgacacca tttaccatgg tcataaagag
    50221 cccctgcgcg ctggcacgaa tgctggaatc aacttcctgt tcgacaaata ccgaaccaga
    50281 aatattgaag aaatcgaatg cacagccata aacaatcatc gacagcagca gcaaaataaa
    50341 cccggttggt gacggatctc cataagcgaa gaaaccaaag cgcagcgtcc aggccaccat
    50401 actcatcatc atgacggttt tgatgccaaa acgcttcaaa aagaacggaa tcgtcaaaat
    50461 gaaaccgact tcagccatct gtgaaactga cagtaaaatg gagggatatt tcaccacgaa
    50521 gctatcagca aactccgggt tacgggcgaa atcatgcagg aacggattac caaaaacgtt
    50581 ggtaatttgc agtaccgcac ccagcatcat ggcaaagagg aaaaagatgg ccatgcgtgg
    50641 atttttaaac agcacgaagg catccagacc cagcttgctg gcaagcgacg tggccgattt
    50701 tttctccgca accggaatct taggcaaagt cagcgcataa gccgacagca acaatgacgc
    50761 accggacgca acatacagct gcaggctgct caattccaaa tgcagtaagc ttaccgccca
    50821 catcgcgaca atgaatccca ccgtaccaaa aacgcgaatg ggtgggaaag ccgtcaccgg
    50881 atcaagccct gcctgggcca ggcaagagta agagacactg ttcgataatg cgatagtcgg
    50941 cataaatgcc atcgcattga ttaacatcac ccaaaacatc gtatccggat cactcaccga
    51001 cgccgcataa aaaagtacgc ccgcgcacac cagatggcac agcatatatg ctcgttcagc
    51061 gcgcagccat ttgtccgcga tgatccccat aatgccaggc ataataatcg cggcaatacc
    51121 tttggagctg taaaccatgc cgacattagc gccggtaaaa tggagggtat taatcatgta
    51181 agaaccgagg gtaaccagcc agctccccca gataaaatat tgcaaaaaga acattacctt
    51241 taagcgcaaa gcgatgctca taatcgtttc cttgtcgcat catgtggcct cacccgtgag
    51301 gccgtttatt gttgttttaa tcaggcaatt ttgcgcagaa acccgcaaat taggttgata
    51361 aagttctgct tagaaagctc cgctgctgcc agcgtttggg tatgcgagag cttcacgtca
    51421 ctcaggcctt ctgccatatt ggtaatcgca gaaacggcca ctaccttcag gtcacaatgg
    51481 cgtgctgaaa taacttcagg cacaacggac attccaacga catccccgcc aataatttgc
    51541 atcatgcgaa tttcagccgc cgtttcgaaa ttcggccccg gatacgaaac aaacacacct
    51601 tccgtcaggg ggaatccctc ttctttcgcc actttttgca acagcgcccg gtaatccgca
    51661 tcataggcat ttgccagtga gaagaaacgc tctccaaaac gatcatcgtt aagtcccacc
    51721 atcggcgtac ctggcatggt gttgatatga tctttcaatg cgaccaggct gcctgccccc
    51781 acttccggac gcagcgagcc tgccgcattg gtgcagaaca gtagctcgca gcccagcaac
    51841 ttaaaggtac gaattgcgtc agtcataatg gtcattccac gaccttcgta gaaatgtccg
    51901 cgacctttca tacataccac cggtacgccc tggagatggc ccagcactaa ctcccccgca
    51961 tgaccatgta ccgtacttac cgggaagccc ggcagctttt cgtaggaaat tgcgaccgcg
    52021 ttctcaatct gatcggccag cgcgcccagc ccggaaccta aaataaaggc cactcgtggc
    52081 gtgaaatcag gtttataagt cttgataata tctacgcaat acagtgggtt atgagaaaac
    52141 cgagcctgag acatatatat cctttttttg tagggtggaa tctaacgcct ttttataacg
    52201 agattgccct acctcatacc aatacacttt tttcatgtat ccgatagtta ttcgatatcg
    52261 atatatttat attttttcac tattaatagg cggaaaaaac tgtattggta ctctcctttc
    52321 cattgcatta acaatatgcc gcttttaatc aaccttttta ttaagcaatc aactaataac
    52381 gatcggcata gggaattatg ctatgaaaaa acatcttttt actctgacac tctcttctgt
    52441 attggcgata ccagcagtaa cccatgcaga atttaaaggc ggttttgctg atataggtat
    52501 tcattatctt gactggacta gcagaaccac tgagaaatcg tcgaccaaat cacacaaaga
    52561 tgattttgga tatctggaac tggaaggcgg cgctaacttt agttggggag aaatgtacgg
    52621 gttcttcgac tgggaaaact tttataatga tcgtcatgat aaaccgggta gcgagcagcg
    52681 ttatacattt aaaaatacca accgtattta cctgggtgac accggattta atctctactt
    52741 gcatgcgtat ggcacctacg gttctgcgaa tcgtgtgaat ttccatgacg atatgttcct
    52801 ttacgggatc ggctacaatt tcaccggcag cggttggtgg ttcaaaccgt tctttgcaaa
    52861 acgctataca gaccaaacct attataccgg cgataacggt tatgtcgccg gttgggttgc
    52921 aggttacagc tttatgctgg gcagtgagaa attcaccctc accaactgga acgagtacga
    52981 gtttgatcgt gatgccacct acgcggcagg taatggcggc aaagaaggtc tgaacggtgc
    53041 cgtcgcactt tggtggaatg caacttcata catcaccaca gggattcagt atcgttatgc
    53101 ggatgacaaa ctgggcgaag atttttacca ggatgcgata atctactcca tcaaatttaa
    53161 tttctgattg attactgccg gggctcccgg cagtatgaat agctctatat ctgatacagc
    53221 ggatcccctt cccgcccagc cattagcgca cggccctggc ctcgtaccac gtcccataac
    53281 gcttgtgcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttcc
    53341 accaccggcg taatgcgctt taccaccagc gggctacctt caggcaatgg tagcgccaga
    53401 gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatga
    53461 ccaatctcct gcacaatatt cgcctgaata ccgttgcgcg ccagagctgc gtcaatcagc
    53521 ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag
    53581 ggtacataat cttccgcggc caaagcgcta tcgcgatggc aaagcaagaa gaaaggctcg
    53641 gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaaa
    53701 tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggata
    53761 aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg
    53821 gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg
    53881 gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc
    53941 tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta
    54001 tgattttcca gctccttcac actgtgactt actgccgact gactcaggcc gatacgctct
    54061 cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgctttaaa
    54121 gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact
    54181 ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct
    54241 cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag
    54301 aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc tgctgttctt
    54361 tatgcacggc gcgaagttgt cgcgtgaagc gattattgct ggcggtggtc actggcgact
    54421 gcatttgtgg gtaatgtgca gcacctttgt actgtttccg attctgggcg tgctgtttgc
    54481 ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcat
    54541 tctccccgct accgtgcagt ctgcaatcgc ctttacttca atggcgggcg gtaacgtcgc
    54601 ggcagcggtt tgttctgcgt cagcatccag cctgttgggg attttccttt caccattgct
    54661 ggttggtctg gtgatgaatg ttcacggtgc gggaggcagc cttgagcagg tcggtaaaat
    54721 tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga
    54781 ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt
    54841 ggtttataca gcgtttagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg
    54901 atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa
    54961 cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg
    55021 tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattcttt tccccacatc
    55081 ggtgatcggt atgatggtgc tgcctctgat gattttccat cagatccaat tgatggtctg
    55141 tgcggtgctg gcgcgtcgat acaaacacca gaccgaacag ttacaggcgc agcaggaaag
    55201 ccgcgccaat aaagcttaaa gaggacgctt caggggctgg accagttgcg tcagcccctc
    55261 agttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgctg ggaactcatc
    55321 tttgcgggca aaccacagaa gatactcctc cggcaggtcg attaagcgac gccctttgta
    55381 tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc
    55441 tacccagcaa acgcagcatt tccgcttcgt cgataacttc tatgcccagt tcctgcgctt
    55501 tcgccagttt agaacctgcc gcttctccag ctatcaccag atcggttttc ttcgacacgc
    55561 tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgtgacatct
    55621 ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg
    55681 cgttaatcac caccggcgca ggccagtgaa cgccttccgc caacagctcg ctgatgacat
    55741 tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat
    55801 caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat
    55861 atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga
    55921 agcgagcaaa ggtggtttct ttcgcttttt ccagcgcatt aaccacgttt tgtgccgatt
    55981 ttggccccat acgctccagt ccggtaagtt tgcctgcggt gagtttgaac agatccgccg
    56041 gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc ataccgtcaa
    56101 cgtccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa
    56161 tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac
    56221 ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca
    56281 cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa
    56341 tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca
    56401 cgcctgcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa
    56461 cctgaaactc gacgtcacgc acaaaggtca tctgttcctg cgccgggaat ttaaacgcta
    56521 ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgtgtc agtgagttga
    56581 ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt
    56641 gatagaacgc cagcacttct tccgccgatt cacaaagtgt tacccggtcg ctgaccggca
    56701 aaccccactt tttaaattgc agtaaacggc caagatgagt attcggcagc tcgccacctt
    56761 ccagaatacc gacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat
    56821 caagctgacg cagtgaacca gcagccgcat tacgtgggtt agcaaacact ttcccgcccg
    56881 tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac
    56941 gcacttccag acgcgccggg atattctctc cgtgtagctt cagcggaatg gcgcgaatag
    57001 tacgcacatt agacgtaata tcttccccgg tagtgccatc gccacgcgtc gcggcactga
    57061 ctaaaacgcc attttcatac agaatactga cggcaagacc atccagcttc agctcacagc
    57121 accaggtgac tttctcgttg cttttcagac ggtcctgcac acgtttgttg aaagcaagaa
    57181 agctttcttc atcaaaaacg ttatccagcg acagcattgg cacttcatgg cggatctggc
    57241 tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt
    57301 ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg
    57361 gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca
    57421 gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga
    57481 caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg
    57541 acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga
    57601 tcgtcaagca ctacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc
    57661 ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga
    57721 gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggcta
    57781 aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca
    57841 aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg
    57901 tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact
    57961 ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta caggctctgc aggctggaaa
    58021 gcctgttgtg ccggttgcgg tgctgaatgc actggctgcg gcgctgatgc gacctgtggc
    58081 gaaactggct gctgcaacgg ttgttcaggc tgcggctgat aggcaggctg ctgcacgggt
    58141 tgctgcaccg gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc ttcaggcggc
    58201 tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg gtgttgcggc
    58261 gacggacgag cagcctcatg ctcctgggcg ttagccgggg catgattcac gcggtgaaca
    58321 cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt
    58381 tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg
    58441 gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc
    58501 agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa
    58561 catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta ttaactttca
    58621 cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc
    58681 cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc
    58741 atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta ctattttgcg
    58801 caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt accgctgctg
    58861 gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacgcaact cgatgtctgg
    58921 atcccgactc tcatgagtta tgttccggac tggttacaat ggctgagcta tcttttgtgg
    58981 cctctggcag tcatctccgt gctgttagtg tttggctatt tcttctcaac gattgctaac
    59041 tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg attgactggc
    59101 gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg aatcatgaaa
    59161 cgcgaatggc aaaaatttgc ctggtatctg ccgcgcgcaa ttgtattgct aattctttac
    59221 ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg
    59281 atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc gtttaaagag
    59341 atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc
    59401 ctgtttacga tgatccctct gcttaatctg ttcatcatgc ccgttgccgt ttgtggtgcg
    59461 acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta acaatctacc
    59521 ggttattttg taaaccgctt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc
    59581 ttgcatgtca ttatttccct tctgcatata gatatgctaa atccttactt ccgcatattc
    59641 tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag
    59701 taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg
    59761 catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa
    59821 gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg
    59881 cgttgaactg gttgaaccaa ccagcggtaa taccgggatt gcactggcct atgtcgctgc
    59941 cgctcgcggt tacaaactta ccctgaccat gccagaaacc atgagtattg aacgccgcaa
    60001 gctgctgaaa gcgttaggtg caaacctggt gctgaccgaa ggtgctaaag gcatgaaagg
    60061 cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca
    60121 acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggagatctg
    60181 ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct
    60241 gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc
    60301 cgttgagcca accgattctc cagttatcgc ccaggcactg gctggtgaag agattaaacc
    60361 tggcccgcat aaaattcagg gtattggcgc aggttttatc ccggctaacc tcgatctcaa
    60421 gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cccgtcgtct
    60481 gatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg ccgcggcgtt
    60541 gaaactacaa gaagatgaaa gctttaccaa caagaatatt gtggttattc taccatcatc
    60601 gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga aagaattgca
    60661 acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc ttttcaggtg cttttttgtg
    60721 gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa
    60781 ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg
    60841 attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg
    60901 tcagcggtca acacccgcca gcaatggact gtattgcgct cttcgtgcgt cgcgtctgtt
    60961 aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac
    61021 aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct
    61081 ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact
    61141 tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct gtttaaactg
    61201 cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg cgaagacgag
    61261 cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc
    61321 ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg
    61381 gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa
    61441 aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt cgtgccaagg
    61501 catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt gaagaaaaag
    61561 aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag caggaaatca
    61621 tagccctgat taaagataag cacatgacag ctgacgcagc tgctcatgaa gttatcgaag
    61681 gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt gcggctgacg
    61741 tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt atcgacctga
    61801 gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc gaaactgcac
    61861 agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt acttcccaca
    61921 cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt agcgtcacct
    61981 ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag gtttacgtca
    62041 atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg gcttctgaaa
    62101 aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt caccaggtag
    62161 aagtgtgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt aacggcgctg
    62221 aaggcgttgg tctgtatcgt actgagtttc tgttcatgga ccgcgacgcg ctgcccactg
    62281 aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg caggcggtta
    62341 tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac ttcccgaaag
    62401 aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt aaagagatcc
    62461 tgcgcgatca gctccgcgct atcctgcgtg cctcagcttt cggtaaattg cgcattatgt
    62521 tcccgatgat catctctgtt gaagaagtac gtgcactgcg caaagagatc gaaatctaca
    62581 aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc ggcgtaatgg
    62641 tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt gatttcttta
    62701 gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt aatgatatga
    62761 tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag caagttattg
    62821 atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct ggcgatgaac
    62881 gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc gccatttcta
    62941 tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag gtgttagcag
    63001 agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac aagttcattg
    63061 aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta ggagaagatc
    63121 atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga taccggaact
    63181 attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc
    63241 gtttttgcgg aaaaaatcgt tggtgatggc attgctatca aaccaactgg aaacaaaatg
    63301 gtcgcgcctg tagacggcac cattggtaaa atctttgaaa ccaaccacgc gttctctatc
    63361 gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa
    63421 ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc
    63481 attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt
    63541 atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt
    63601 gaaaccccgg ttatccgcat ca
//
LOCUS       DAOTRK010000010        55846 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.010, whole genome shotgun sequence.
ACCESSION   DAOTRK010000010 DAOTRK010000000
VERSION     DAOTRK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.010"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(17..1102)
                     /gene="dmlA"
                     /locus_tag="QIE41_000701"
     CDS             complement(17..1102)
                     /gene="dmlA"
                     /locus_tag="QIE41_000701"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDS3529622.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEICW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            1205..2128
                     /gene="dmlR"
                     /locus_tag="QIE41_000702"
     CDS             1205..2128
                     /gene="dmlR"
                     /locus_tag="QIE41_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDS3529623.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQLLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2255..2893
                     /gene="leuE"
                     /locus_tag="QIE41_000703"
     CDS             2255..2893
                     /gene="leuE"
                     /locus_tag="QIE41_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDS3529624.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            3066..3425
                     /gene="yeaR"
                     /locus_tag="QIE41_000704"
     CDS             3066..3425
                     /gene="yeaR"
                     /locus_tag="QIE41_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDS3529625.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            3429..3611
                     /locus_tag="QIE41_000705"
     CDS             3429..3611
                     /locus_tag="QIE41_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDS3529626.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            3759..4007
                     /locus_tag="QIE41_000706"
     CDS             3759..4007
                     /locus_tag="QIE41_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDS3529627.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(4066..4140)
                     /gene="yoaJ"
                     /locus_tag="QIE41_000707"
     CDS             complement(4066..4140)
                     /gene="yoaJ"
                     /locus_tag="QIE41_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDS3529628.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4143..4241)
                     /locus_tag="QIE41_000708"
     CDS             complement(4143..4241)
                     /locus_tag="QIE41_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDS3529629.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(4274..5299)
                     /gene="dgcP"
                     /locus_tag="QIE41_000709"
     CDS             complement(4274..5299)
                     /gene="dgcP"
                     /locus_tag="QIE41_000709"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDS3529630.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLIVSLNNENADISSLRERI
                     QQQICGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            5482..5736
                     /gene="yoaF"
                     /locus_tag="QIE41_000710"
     CDS             5482..5736
                     /gene="yoaF"
                     /locus_tag="QIE41_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDS3529631.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(5758..6105)
                     /locus_tag="QIE41_000711"
     CDS             complement(5758..6105)
                     /locus_tag="QIE41_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDS3529632.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6160..7341)
                     /gene="nimT"
                     /locus_tag="QIE41_000712"
     CDS             complement(6160..7341)
                     /gene="nimT"
                     /locus_tag="QIE41_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDS3529633.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     FLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDTNGDWSIPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            7438..8259
                     /gene="nimR"
                     /locus_tag="QIE41_000713"
     CDS             7438..8259
                     /gene="nimR"
                     /locus_tag="QIE41_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDS3529634.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTSTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(8216..8662)
                     /locus_tag="QIE41_000714"
     CDS             complement(8216..8662)
                     /locus_tag="QIE41_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDS3529635.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            8832..8936
                     /locus_tag="QIE41_000715"
     CDS             8832..8936
                     /locus_tag="QIE41_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529636.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(8937..9440)
                     /gene="yeaK"
                     /locus_tag="QIE41_000716"
     CDS             complement(8937..9440)
                     /gene="yeaK"
                     /locus_tag="QIE41_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDS3529637.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTV"
     gene            complement(9483..10973)
                     /locus_tag="QIE41_000717"
     CDS             complement(9483..10973)
                     /locus_tag="QIE41_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDS3529638.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(11154..12629)
                     /locus_tag="QIE41_000718"
     CDS             complement(11154..12629)
                     /locus_tag="QIE41_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDS3529639.1"
                     /translation="MIRSTRISLGLFFNYFLSSTKIDPGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILNNNKKKTGILLLALIPFLVFPLPAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            complement(12776..14059)
                     /locus_tag="QIE41_000719"
     CDS             complement(12776..14059)
                     /locus_tag="QIE41_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDS3529640.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(14172..16106)
                     /gene="yeaG"
                     /locus_tag="QIE41_000720"
     CDS             complement(14172..16106)
                     /gene="yeaG"
                     /locus_tag="QIE41_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDS3529641.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            16542..17288
                     /gene="mipA"
                     /locus_tag="QIE41_000721"
     CDS             16542..17288
                     /gene="mipA"
                     /locus_tag="QIE41_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDS3529642.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            17378..18232
                     /gene="yeaE"
                     /locus_tag="QIE41_000722"
     CDS             17378..18232
                     /gene="yeaE"
                     /locus_tag="QIE41_000722"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDS3529643.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEF
                     GLTLIDTAEMYAEGGAEMVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(18283..19167)
                     /locus_tag="QIE41_000723"
     CDS             complement(18283..19167)
                     /locus_tag="QIE41_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDS3529644.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(19251..20246)
                     /gene="gapA"
                     /locus_tag="QIE41_000724"
     CDS             complement(19251..20246)
                     /gene="gapA"
                     /locus_tag="QIE41_000724"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDS3529645.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            20588..21001
                     /gene="msrB"
                     /locus_tag="QIE41_000725"
     CDS             20588..21001
                     /gene="msrB"
                     /locus_tag="QIE41_000725"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDS3529646.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            21043..21315
                     /locus_tag="QIE41_000726"
     CDS             21043..21315
                     /locus_tag="QIE41_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDS3529647.1"
                     /translation="MNLDDIINSMTLEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKAIAMFK"
     gene            21685..22761
                     /locus_tag="QIE41_000727"
     CDS             21685..22761
                     /locus_tag="QIE41_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDS3529648.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAILDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            22788..24167
                     /locus_tag="QIE41_000728"
     CDS             22788..24167
                     /locus_tag="QIE41_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529649.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVSFGLLVCWSNAVGGL
                     ILAQLKELGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALMTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVVNK"
     gene            24184..25227
                     /locus_tag="QIE41_000729"
     CDS             24184..25227
                     /locus_tag="QIE41_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDS3529650.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKAGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDMMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATDIAVVDVLDKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEEMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYQDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            25248..26084
                     /locus_tag="QIE41_000730"
     CDS             25248..26084
                     /locus_tag="QIE41_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDS3529651.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAKKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDTDIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            26089..27036
                     /locus_tag="QIE41_000731"
     CDS             26089..27036
                     /locus_tag="QIE41_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDS3529652.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNCNGSLWKLNIDDVDFSRFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGASTGVKNRKLVEQLLEECEG"
     gene            27046..28026
                     /gene="ydjG"
                     /locus_tag="QIE41_000732"
     CDS             27046..28026
                     /gene="ydjG"
                     /locus_tag="QIE41_000732"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDS3529653.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIRKEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKDE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMESELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            28163..28921
                     /locus_tag="QIE41_000733"
     CDS             28163..28921
                     /locus_tag="QIE41_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDS3529654.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            29039..30397
                     /locus_tag="QIE41_000734"
     CDS             29039..30397
                     /locus_tag="QIE41_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529655.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIVILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSVRLPPLTLNQSNSKANVINGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(30490..31131)
                     /gene="pncA"
                     /locus_tag="QIE41_000735"
     CDS             complement(30490..31131)
                     /gene="pncA"
                     /locus_tag="QIE41_000735"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDS3529656.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(31142..32158)
                     /gene="ansA"
                     /locus_tag="QIE41_000736"
     CDS             complement(31142..32158)
                     /gene="ansA"
                     /locus_tag="QIE41_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDS3529657.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(32323..34179)
                     /gene="sppA"
                     /locus_tag="QIE41_000737"
     CDS             complement(32323..34179)
                     /gene="sppA"
                     /locus_tag="QIE41_000737"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDS3529658.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGVLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            34340..34891
                     /locus_tag="QIE41_000738"
     CDS             34340..34891
                     /locus_tag="QIE41_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDS3529659.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAGGSDDKAIDKARNAPYRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35008..36051
                     /gene="selD"
                     /locus_tag="QIE41_000739"
     CDS             35008..36051
                     /gene="selD"
                     /locus_tag="QIE41_000739"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDS3529660.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            36056..38011
                     /gene="topB"
                     /locus_tag="QIE41_000740"
     CDS             36056..38011
                     /gene="topB"
                     /locus_tag="QIE41_000740"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDS3529661.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(38088..39431)
                     /gene="gdhA"
                     /locus_tag="QIE41_000741"
     CDS             complement(38088..39431)
                     /gene="gdhA"
                     /locus_tag="QIE41_000741"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDS3529662.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            39667..39939
                     /locus_tag="QIE41_000742"
     CDS             39667..39939
                     /locus_tag="QIE41_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDS3529663.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(39905..40312)
                     /gene="nudG"
                     /locus_tag="QIE41_000743"
     CDS             complement(39905..40312)
                     /gene="nudG"
                     /locus_tag="QIE41_000743"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDS3529664.1"
                     /translation="MKMVEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEAAVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            40399..41019
                     /locus_tag="QIE41_000744"
     CDS             40399..41019
                     /locus_tag="QIE41_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDS3529665.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFVWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(41028..42335)
                     /gene="ynjE"
                     /locus_tag="QIE41_000745"
     CDS             complement(41028..42335)
                     /gene="ynjE"
                     /locus_tag="QIE41_000745"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDS3529666.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQILNGPSGHEPAALNLSASWLDKMSTEQLNAWIKLHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(42402..43055)
                     /locus_tag="QIE41_000746"
     CDS             complement(42402..43055)
                     /locus_tag="QIE41_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS3529667.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKCDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAERQIGILFQDALLFDQFSVGQNLLLALP
                     AALKGVARRDAVNDALERAGLGGMFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(43055..44590)
                     /locus_tag="QIE41_000747"
     CDS             complement(43055..44590)
                     /locus_tag="QIE41_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3529668.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQTL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIVFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(44563..45729)
                     /locus_tag="QIE41_000748"
     CDS             complement(44563..45729)
                     /locus_tag="QIE41_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDS3529669.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(45739..46287)
                     /locus_tag="QIE41_000749"
     CDS             complement(45739..46287)
                     /locus_tag="QIE41_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDS3529670.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(46287..46994)
                     /locus_tag="QIE41_000750"
     CDS             complement(46287..46994)
                     /locus_tag="QIE41_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDS3529671.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGALWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSILTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(47009..47686)
                     /gene="ydjY"
                     /locus_tag="QIE41_000751"
     CDS             complement(47009..47686)
                     /gene="ydjY"
                     /locus_tag="QIE41_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDS3529672.1"
                     /translation="MLQHYSASWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVAGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(47691..48401)
                     /locus_tag="QIE41_000752"
     CDS             complement(47691..48401)
                     /locus_tag="QIE41_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDS3529673.1"
                     /translation="MNAERKFLFACLIFALLIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(48568..49374)
                     /gene="xthA"
                     /locus_tag="QIE41_000753"
     CDS             complement(48568..49374)
                     /gene="xthA"
                     /locus_tag="QIE41_000753"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDS3529674.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSTLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            49820..51040
                     /gene="astC"
                     /locus_tag="QIE41_000754"
     CDS             49820..51040
                     /gene="astC"
                     /locus_tag="QIE41_000754"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDS3529675.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLTDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDILHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLHGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            51037..52071
                     /gene="astA"
                     /locus_tag="QIE41_000755"
     CDS             51037..52071
                     /gene="astA"
                     /locus_tag="QIE41_000755"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDS3529676.1"
                     /translation="MMVIRPVERSDISALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            52068..53546
                     /gene="astD"
                     /locus_tag="QIE41_000756"
     CDS             52068..53546
                     /gene="astD"
                     /locus_tag="QIE41_000756"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDS3529677.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGKWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGRRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPAREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            53543..54886
                     /gene="astB"
                     /locus_tag="QIE41_000757"
     CDS             53543..54886
                     /gene="astB"
                     /locus_tag="QIE41_000757"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDS3529678.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTSHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATVAPSTDTLDGKVHLTVANLNNKFHRSLEAPVSESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQVQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            54879..>55846
                     /gene="astE"
                     /locus_tag="QIE41_000758"
     CDS             54879..>55846
                     /gene="astE"
                     /locus_tag="QIE41_000758"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDS3529679.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQTPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT        13968 a        13536 c        14466 g        13876 t
ORIGIN      
        1 ataaactggt ttgaccttaa cgcaaaataa ttttgcaaat cgcgtcggca acctgtggcg
       61 tggtggcatt gcctttcata tcaggtgttt tcggcccgtg agcaatcact tcttcaatcg
      121 cggccagaat accgttatgc gcttgctgga aacgctcatc gccattgccg agaaaatcga
      181 gcatcattgc accggcccaa attgtggcga tagggttagc aatgtttttc ccgtagatat
      241 ccggcgcgga accgtggact ggctcgaaga gcgacgggaa agtgcgttcc ggattcaggt
      301 tggcggatgg ggcaatgcca atggtgccgg tgcaggccgg gccaagatcg gaaaggatat
      361 cgccaaacaa attggacgcc actaccacat cgaagcgttc cggctgcatc acaaaacgcg
      421 cgcagagaat atcaatatgc tgcttgtccc agcagatctc cgggtaattc tcggccattg
      481 cttccactcg ctcatcccag tacggcatgc tgatggctaa accgttcgat ttagtggcag
      541 aagttagcgt cttacgtggg cggctttgcg caagttcgaa ggcataacgc aaaatgcgat
      601 cgacaccacg acgcgtaaat accgattcct gaatgacgac ttcatgctct gtaccttcat
      661 tcactctgcc gccgagcgag gaatattcgc cttcggtgtt ttccctgacc acgtaaaaat
      721 cgatgtcgcc aggctgtttt cccgccagcg ggcagggaac gccaggaaag agacgaaccg
      781 ggcgcaggtt gacgtactgg tcgaattcac gacgaaattt cagcagcgaa ccccacaacg
      841 aaatatggtc cggaacggtg tccggccagc cgacggcacc aaaatagatg gcgtcgaaac
      901 ggctaagttg ctcatgccag tcgtccggca tcattttacc gtgatggctg taatactcgc
      961 agctcgccca ctccatttgc tcaaaactca aggcgaagcc ccagcgctca gcggcagcct
     1021 gtaacacgcg aatcccttca ggaaggactt ctttgccaat cccgtctccc gggatcgcag
     1081 caatacgcat cgttttcatc attgggttct cacttcctga ccatcaaaat tgacaacatc
     1141 accatcctat aattgactga aaaggaatta atccctgtaa ctggtgaaac ataaaacacg
     1201 aatcatgaat aatttaccgc tgctgaacga tttgcgcgtc tttatgctgg tggctcgccg
     1261 ggctggtttt gccgccgtgg cggaagaact gggcgtttct ccagcgttcg tcagcaagcg
     1321 catcgccttg ctggagcaaa cgctaaacgt ggtgttgtta caccgcacca cccgccgtgt
     1381 caccattacc gaagaaggag agcggattta tgaatgggcg cagcggattt tgcaggatgt
     1441 cggtcaaatg atggacgaac tttctgacgt gcgtcaggtg ccgcagggga tgctgcgtat
     1501 tatcagcagc tttggttttg gacgacaggt ggtggccccg gcgttatcag cgctggcgaa
     1561 ggcgtatccg caactggaat tacgttttga tgtggaagac cggttggtcg atttggtcaa
     1621 tgaaggtgtc gatctcgata ttcgcattgg cgatgatatc gcccctaatt taatcgcccg
     1681 caaactggcg accaattatc gcattctctg cgcgtcaccg gaatttattg cgcaacatgg
     1741 cgcgccgaaa catttaaccg atctttctgc attgccgtgc ctggtcatta aagagcgcga
     1801 tcatcctttt ggtgtctggc aattacgcaa taaagaaggc caccatgcga ttaaagttac
     1861 cggcccactg tcgtctaatc atggagaaat tgtccatcag tggtgtcttg atgggcaggg
     1921 gattgctctg cgctcctggt gggatgttag cgaaaatatt gccagtggtc atttagtgca
     1981 gttgctaccg gaatattacc agccagcgaa tgtctgggcc gtttatgttt caaggctggc
     2041 gacgtcagcg aaagtgcgga taacggtaga atttttacgc cagtattttg ccgagcacta
     2101 cccgaatttt tcactggagc atgcctgatt aatgattcaa ttatcgggtt gatatcgggt
     2161 taaaacctga ttttctcctt tctaagccgc tacagattgg ttagcatatt cacctttaat
     2221 cgcgcatgat cgaaagataa ttaaagaggt taatgtgttc gctgaatacg gggttctgaa
     2281 ttactggacc tatctggttg gggccatttt tattgtgttg gtgccagggc caaataccct
     2341 gtttgtactc aaaaatagcg tcagtagcgg tatgaaaggc ggttatcttg cggcctgcgg
     2401 tgtatttatt ggcgatgcgg tattgatgtt tctggcatgg gctggagtgg cgacattaat
     2461 taagaccacc ccgatattat tcaacattgt acgttatctt ggtgcgtttt atttgctcta
     2521 tctggggagt aaaattctct acgcgaccct gaagggtaaa aataacgagg ccaaatccga
     2581 tgagcctcaa tacggtgcca tttttaaacg cgcgttaatt ttgagcctga ctaatccgaa
     2641 agccattttg ttctatgtgt cgtttttcgt acagtttatc gatgttaatg ccccacatac
     2701 gggaatttca ttctttattc tggcgacgac gctggaactg gtgagtttct gctatttgag
     2761 cttcctgatt atttctggtg cttttgtcac acagtacata cgtaccaaaa agaaactggc
     2821 taaagtgggc aactcactga ttggtttgat gttcgtgggt ttcgccgccc gactggcgac
     2881 gctgcaatcc tgatgctttc agcccgcgtt gtcgcgggct tcccatctgt aatcctccct
     2941 gattcttctc tgatatggtg ctaaaaagta accaataaat ggtatttaaa atgcaaatta
     3001 tcaggcgtac cctgaaacgg ctggaataaa ccattttcag cgcattcacc gaaggaggga
     3061 aaaggatgct gcaaatccca cagaattata ttcatacgcg ctcaacgcct ttctggaata
     3121 aacaaactgc gcctgccgga atattcgaac gtcatcttga taaaggaacg cgcccgggag
     3181 tttacccacg cctttccgtt atgcatgggg cggtcaaata tctcggctac cctgatgaac
     3241 acagtgcaga gcctgatcag gtgatcctta tcgaagcggg gcagtttgcg gtgttccctc
     3301 cagaaaagtg gcacaacatt gaagccatga ctgacgatac ttatttcaac attgacttct
     3361 tcgttgcccc tgaagtcctg atggaaggtg cgcaacaacg gaaagtcatt cataacggga
     3421 aatgaatcat gggcaaagca acgtataccg tgaccgtcac caataacagc aatggcgttt
     3481 ctgtcgatta tgaaacagag acgccgatga ctttgctggt gccagaagtg gcggctgaag
     3541 tgataaaaga tctggtgaat accgtgcgtt cttatgacac ggaaaacgaa catgatgttt
     3601 gtggttggta attatcactg atcgttgatg gcgcaatttc tgcgccatcg caaaaaaatc
     3661 ctccgtgatg ctcgtcacaa atcttgccct cccgatgttg tgctatgctt tttatcagcg
     3721 actaacccgc taatgcaaaa cagataagtg aggaaacaat gggcattctg tcatggatta
     3781 tttttgggct tattgccggt attctggcga agtggatcat gccaggtaaa gatggtggtg
     3841 gattctttat gactatcctg ctggggatag tcggtgccgt agtcggcgga tggatcagca
     3901 cgctgtttgg ctttggtaaa gtcgatggct tcaattttgg cagcttcgtg gttgccgtta
     3961 ttggcgcgat tgtcgtgctg tttatctaca gaaagattaa aagttaacgc gtaaattgca
     4021 caaaggctgc acacaggcag cctttgctat tttctagatg tgacgttacc accatcccag
     4081 ctcagtgccg agtacgacaa taatcgccac acccatcata ataatcgttg tttttttcat
     4141 ctttatgctc ccggggcagc atagccgcca ataaaaaacc atgctaaaaa tacgaccgct
     4201 gtaataaaga ttacaaccgg aaaaataata ccgattcgca tgttatcacc aatcaaaagg
     4261 atgtgaatac gcctcaggaa tgtagcgctg gatgcgcgac aaaaggtgtt ttctgtttgt
     4321 gttttttctc ctgatacatc gcgatatcgg cagcatgcag ggcactgtct gcatcggtcg
     4381 tttcaggatc gacttctact atgccaagac tggcaccggg ataatacaaa tcaacatcac
     4441 ctaagtgata ttctccgcat atttgctgct gaatacgttc tcgcagagac gaaatatccg
     4501 cattctcgtt gttcagtgaa acaatcaaaa actcatcacc gccgagacga ccaataactt
     4561 cgccattttg ctggagcgta ttaaggcgct cgccaacctg aatgaggaac aaatcaccac
     4621 tattatgacc aaaacgatcg ttgattaatt tgaagttatc cagatcgata aacgcgatca
     4681 ttatcttatg gttaagatgt cgggcgagag aaaacagcgt cgtcaggttt tcaaaaatcg
     4741 cccgccgatt cggtagcccg gttaacgagt cggtatacga ttgcgcaatc agcgcagcat
     4801 tggcttcgcg cagctgttca accagtgcct ctttttgaat atattgtgca atcagtccgg
     4861 cgaataactg taaaacctgt tccgcgcgtt cactccactg gcgcttctca ctactggcgg
     4921 cgcaaagcgt gccatagaat gatccatccg gtaagtgaat tggcgtgctc agaaatgtgg
     4981 tgatgccaag attgcgtgcc gcaatacagt cgccccaacg gtcagggact tcatcgctga
     5041 aaaagcagtt ttcatcaatg gcgcgtttgc ataacgagta atcccacgag acggtaaaat
     5101 tctccgggat gtgcattttc tggctgttac gggcaaacat aatatgctgc aggcgcgctt
     5161 cgacatccac tttggtcagg taggttgatt ccatatcagt gaccatttcc agcatctcca
     5221 gaagctgtcg gaccagactt tccagtgact gttctttggc aagggattgc gagacgcggg
     5281 cgataatctg atctgacacg aaacaactcc aggatacggc gggtaaatgc tgattattta
     5341 atctttgcag gcatgataaa acatcatcaa caaagatgaa acaaggcgta atatgatttg
     5401 agtgaatgta ataaaaatag tgctgcaaaa caaaacgata ccgcgatact ctaagtctgg
     5461 ataaatatgt gagatcaata aatgaaaatt atcagttttg ttctgccttg cttgctggtc
     5521 ctggctggtt gttcaacccc ttctcagcca gaagcaccca aaccgccgca gattggtatg
     5581 gcaaatccgg cgtcggtcta ttgccagcag aagggcggga cgctcattcc agtgcagaca
     5641 gcgcaagggg tcagcaacaa ttgcaaatta ccgggcggtg aaacgattga tgaatgggca
     5701 ttgtggcgac gggaccatcc ggctggtgaa aaataaccgc cggatgctgt actaaaatca
     5761 caagctacgt agccagtcgg ccagcaccag cgcatggttc tgcgtggtgt tttttgctga
     5821 gtagagcagg gtcagcggct gttttttggc gatgtccgcc agccgctttc cttcttgctc
     5881 gtgttgcgct agttctgcaa gatattgctc gcgaaaggtt gcaaaatcga cgacttcgcc
     5941 gtgaaaggct ttgcgcagtt ccgttgacgg cgtgatttct ttatcccact catcaagggc
     6001 taaatcggtt tttttgatac cacgcggcca gaggcggtcg accagtacgc gataaccatc
     6061 gctctgttct gccggatcat aaacgcgttt gcactgaata ttcatcgtgt ttgttctccc
     6121 ttccacgcat gattggtcac gtcactatag caccggatat tagcgaattt ctttgtctct
     6181 cccggcgcaa agtccgaaaa tcgccatcag tagtgaaagt atggcaacac ccatcaatgg
     6241 tatggaccag tcaccgttag tatcgtgtat tttacccatc agcggcggcc cacaggctgc
     6301 cagcaaatac ccgacggatt gtgccatccc cgagagtgcc gccgcctgat gcgcagaact
     6361 tgcccgaaga ccaatgaacg tcaatcccag tatcattgtt gcgccggaac caaaaccgaa
     6421 aagcagagtc caggtgatcg cgtgcgctgg cataaagcag agcccaaccg cgcccactgc
     6481 gcacatcaag gcaacgaacg ctgcaatacc acgctgatct ttcacatgat gtaagaaaag
     6541 tgggatcagc aaaccgggag ctgctgtggc tagttgcagc aaaccatgca gtgaacccgc
     6601 ctgtgcttcg ctatagccgt ggctgatgag gatcgccgga agccagccaa taatcacgta
     6661 atagaccagt gagttgatcc caagaaacaa tgttacctgc caggcaagcg gcgaacgcca
     6721 gataccccga gtatgtaagg cgcgtgaggt actcaaattt gcatgttgtt gacttcgcca
     6781 ctgtggcagc cataaaaaaa gagccagcag aggaaaagac atcagcatga gcaacgcgcc
     6841 ttgccagcca aaaccgttca aagccagcgg cacgaccata gccgatccca gtgccgctgc
     6901 agctcccatt gtcagggaat atgcgccggt aagtctggcg acggaatgag ggaaatcgcg
     6961 tttaattaat cctggcagta agacattgcc taatgcaatc ccaccgccaa tgaccgctgt
     7021 accgccaaat aagaagtaag gcgaagggag agagcgaatt gcgatgccag cacagatcaa
     7081 aagtaacgcg gcaaacaggc tacgttccat accaaaacgt cgcgccaccg gggcagccag
     7141 aggtgagatt agcgcaaagg ctaataatgg cagggtggtc aataagccgg tttgcgccgt
     7201 cgtcagcgag taagcggaac gaatcgtatc cagtaacggt gctgcgccgg taaaggtgac
     7261 gcgtaatgtt gtggcaatca ttaaaatgcc agcgataagg acaatcctgt ttttgccgct
     7321 taatgaagtt gaacaggtca tatgcttctc attgttacaa acgaggctgt accttaccgt
     7381 ttttattgtt atgttgaatc aagctaaaat gacaaattat cgctaaattc ggacaaaatg
     7441 atgcacaggt tgaatctcaa tggctatgaa cccgaccgac accatgaagc cgcggtcgca
     7501 ttttgtattc acgccggaac ggatgagtta accagtcctg tacatcagca ccgcaaagga
     7561 cagctgatac tggcgctaca tggtgcaatt acctgtacgg tggaaaatgc tttgtggatg
     7621 gtgccaccgc aatatgcggt ttggataccg ggcggagtgg aacatagcaa tcaagtgaca
     7681 gctaacgctg aactctgttt tctgtttatc gaacccagcg cagtgacaat gccgacaacc
     7741 tgttgcacat tgaaaatatc gcccttatgc cgggaattaa ttttaaccct ggcaaacagg
     7801 acgtcgactc agcgtgctga gccgatgacc cgcagattaa ttcaggtcct gtttgatgaa
     7861 ctccctcagc agccacaaca acagctgcac ctacccgttt cttctcatcc taaaattcgc
     7921 gcgatggtgg agatgatggc gaaagagccc gtcgagtggg ggtcattggg gcaatgggct
     7981 cgcttttttg cgatgagtga acgcaatctt gcccgactga tagtgaaaga gacggggttg
     8041 agttttcgcc agtggcgtca gcagttacag ctgattatgg cgcttcaggg attagtgaaa
     8101 ggcgatactg tacagaaagt tgctcatacg ctggggtatg attcaacaac ggcctttatt
     8161 actatgttta agaaaggcct ggggcaaacg ccaggccgat atattgcggg attaactact
     8221 gtttccccac aatcagcgaa accagacccg cggcaataag cgggccaacc ggtacgccgc
     8281 gaaacagcgc cacgcccaaa acagtgccca ccagcagccc ggcgaccagt tgcggctggc
     8341 tgcccattaa cgtcacgccg cgcccaccta gccaggagac aataacgcct actgcaattg
     8401 ccaccagcga tttccagttc agaaacgagt gaatcagggt tgagggcggt agcgtgccgc
     8461 tggcgatggg ggccataacg ccaatcgtca gaatgattat cccgatactt agcccctgtt
     8521 tttcaatcca cgggaaaaag gtgcttaacg gtgtgacgcg gacaatgatt aacaccagaa
     8581 ttgaaacggc gacagtggtg ttatgactga taaagcccag cgcagctaac ccgagcagga
     8641 tcagcaaagt gacatcaaac atcgtggttt ccttgccaaa gaataataag cctgcttact
     8701 ttacgcttgc tgcaggaagg aagtagaaaa tctttacgtt ttttgcataa aaaatcaggc
     8761 gaactgtcat caaactgcca tttgcgcacg ctaacaataa cgtgaaaaca gctatctccc
     8821 gggggtatac tatgaacgat caaatgttcg tcgagacact gattatcacg tcatcgtttt
     8881 ttgccattgc tgttgtgctg gttttgtccg ttcttcttat cgaacggaca ggctagttac
     8941 acagtgcggc ggaaattgac cagttctggt tgcgcaatgc gcaaataatc cgcggttttc
     9001 aaaataacgg atttatccag catgcctgca ttgaaggcga tttcatcaaa tcgttcgaag
     9061 agtaggggat cggcaaccag tttgagtttt ggatggaagc tgaaaggggg gatcgcgccg
     9121 aagacacagc ctgtgagttc atcgacttcc gccgggctgg ccaacgaagc gcgtaatccg
     9181 cctatatggc tggcgagttg gctcagatcg gcctgttgat cggccgcgag gatcgccaga
     9241 acatgttgat ttacaccatt gcctttgact ttacatacca gtgccttagc cccctggcct
     9301 aaagcggtgc cacgaatttc tgacaccgct tcgcatttac ctaccgcttc atgggtaacg
     9361 acgcggaagt cagcaccttc ttgtgataat aaagcgatta accgctgatg cgtcacgctt
     9421 cctttagcca tttcagtcat ttcatgctcc tgagattaca agcaaacaac cacagaagat
     9481 tatcatgaac ggctgttttt gttctgctta ttgacatata aacgctcatc ggaagcttta
     9541 tacgcatcat gtaacgtatc gttttctttc atcgcgtaaa taccggaaga gaagccgatc
     9601 tctttctgcg gcgcgatatg ttgcagacgt ttttcgatac gttcaggcag ttgtgctgca
     9661 atttgcggcg tcgaatcgac aagaatgatg cagaattcat cgccaccgag tcggatggca
     9721 taatcacttt tacgaatcga ctgtttaatc gcctgagcta ataacgtaat cgctaaatcc
     9781 ccctcctgat gaccaagggt gtcatttatt tgctttaact tgtccatgtc aattgcaata
     9841 aacatcaccg aagaaccgga ttgcaccagt ttttgcaacc gctgctccag ttcaggggtt
     9901 aaaattttgc gattatagag tccagtcatc gcatcactaa tattttctcg cgacacattt
     9961 tggtataaac ggaagtgcat ccgcaccata ttcagcaaaa ttaacgccgt caaaatccag
    10021 aacagaacgc ttttccacga agacgtgata aagtaaagaa tatcaatgga taacgagaca
    10081 cgaatgccgc ccggtaagtc atggacgtaa ctgacatact ggaacagatt atcttcgctc
    10141 tggttgatga taatgtcgcg ccccgaatcg gtatcggtta gcgtgacatt gagaaaacgc
    10201 cagaggagag ggcggtcatg agtgtaaaag atattccgca gattgttttt gttaatatcc
    10261 agcagcacaa tcccttttag ctgccctgcg acatataccg gcgtcagcaa actcatcacg
    10321 ttctgtttag tacgctgatc ctggtaaatg ctcgataata cggtacgccc ggtaaatacc
    10381 ttttcaatat ctttgcgatc gatgccaata cttcctgact gcaggaagga ccagttattc
    10441 atcgcaaagt attcaacatt aaccggctca taaaaataga catagcggtc attgagatcg
    10501 agataatagt gaaatttctc ttcggccatc ccaagacctg aaccgtaatc atcctggtgg
    10561 cggttgcctt ccactgcctg atcaaaagcg ggtaatagtg cagcagcttc cacaattgtg
    10621 tcgcagtcag tggatgcggt ttgtagtgtg ccgcgtaagc ccgggtattt atgggcagtc
    10681 aggtttaagc catgggtatt attggcagac tcaaaagcct cgcagatggc gcgccgctgt
    10741 tctggcgaca cttccgactg ctcagcagcg agagcgcgca tcacatgcgc ggcaatactc
    10801 tgattctgat acttatcata aagaaaggag gagtctgctc tctgcacgat atagcgcaga
    10861 taggcactca ttgctctgtc gctggcgaca agttcaaaaa taaggaagga agatgtcagc
    10921 acaatgacac ttgcggcgat aaaatgccgg agcattctat ggtgcaattt catgataaat
    10981 ggcactcctt cggttagtga aacgagaaaa ccagataaag ttcgattgcg gcctggcaca
    11041 ggggcgctaa ttatactcat ctctgcgccg ctggggaggg gaaatttagg ggtaatagta
    11101 ataaattgta tgcaaaagcc acgcaaaaag catggctctg aatatttttg cacttaaata
    11161 tgatgaataa tcactttgtt gcccccctcg cttttcgctt cgcgtaaagc tttatctgct
    11221 tccgtcatga caagcgagat attacgtgtg tcacccgttg aaaaaaccgt gccaatactg
    11281 atggtcattt gctcaggaac atcatatctg ttactaaagc cggtcaggag ttcgacattt
    11341 ttacgcatac gttccgcaat gatttttgcc tgggcactat tgagttcggt aaatagcaag
    11401 ccaaacacct caccctccag tcgcgctaaa atatcatcgg ggcggatgct ttgctggata
    11461 atatcgacaa ttgaaaccag cactttatca cccacacgat gtccccattg ggcattgatg
    11521 cgtttgaact gattaatatc aaccagcatg acacagaagt ccttaacaac aggtcgcgac
    11581 aataatgact ctacgctgtt gaaaaagtag cgccgattat aaatattggt cagtacatca
    11641 tgaactgcca gtttgctgga gagttgcagt tcctgaaaaa tgttataaat cagaaaagac
    11701 accacaaaca gtttactgga aacttcaatc ccgcgactga tataccagat ggtataatta
    11761 tactcatcca gagtcagtag aagtaagttg cagcatagcg atgccagaca taataatgct
    11821 attaacggcc atagttcgct ggctaatcgt gtacgcatga taataaagaa cagtaaaaat
    11881 gcccacagac aaaccaatat ttttgtatag ttgattcccc aggtcgcagt atggttgtcc
    11941 ggacagtaat cgacgacata caaagaatag tcagcgttat aactgctcag attgtgtgcc
    12001 ggaaggggaa aaactaaaaa agggatcagc gccagcaaca gtattcccgt ttttttctta
    12061 ttattgttca ggatgttgtc tttaccataa caaaagagcg ccagcgaggt taaacaaatg
    12121 aaactgagtt ggcggaaaat atagaaaata gaaacatcat tcgactttgt ctggatcagt
    12181 gtactgccgt tgattggttt atggataatt acaatggttt caactaaata gataaggcca
    12241 cttaaaaacg caaggcttaa aatgagtgtt ggtaaaattt gtttgttagt gaaatatttc
    12301 attaacatga aaagaaaaat gacggcgtta aggaaaaaca aagccacaac aaccatcagg
    12361 taattcatct gtgggatgag ttttacattc gtgttttcgt tatataaggt agtaatgcaa
    12421 aataaaatta acgtcagcaa taaactgaaa ataaaaaagc cttgagcttt taatttttgt
    12481 gtacccatag aagccataaa aaggagagca atgtgagtgc tggattttat tgatggcaga
    12541 gatatatagt tttgaccagg atcaattttc gttgacgata aaaaataatt aaagaataac
    12601 cccagggaaa tacgtgttga ccgaatcatt tttccgaatg attgttttct ggaactaaat
    12661 tgtgtaaagg ttatgctgtc atatttaaag cgattgtaag ataatgtatg taataaatga
    12721 gataatttac agtgagctaa aaatcagcca ggcgataatg acccggctga tagttttagt
    12781 ctttagctgt tgcattttgt ttatgaaaca gttcacggaa caccggataa atatcatcct
    12841 ggtcgcggat atgctgcatc gcaaagttgt cgaaagtaga ttgcagatgc tcatattctc
    12901 gccacagtgt ctgatgagcg cgacgggtaa tttcgatata gctgtaataa cgaacgacgg
    12961 gtaataattt tttcgccagg atttcatggc aaagcggaga gtcatcggcc cagttatcgc
    13021 cgtccgatgc ttgtgccgcg taaatattcc actgtgccgg gttataacgc tctttcacta
    13081 cctcatccat cagtttcagg gcgctggaaa caatggtgcc gccagtttcc tgcgagtaga
    13141 aaaactcatg ttcatcgact tctttcgcct gggtatgatg gcggatgtat acgacttcca
    13201 cgttcttata tgttctgctg aggaacagat acagcagaat ataaaaacgc ttagccatat
    13261 ctttagtgga ctgatccatt gaaccggaaa cgtccatcag gcaaaacatc actgcctggc
    13321 tggagggatc gggccgcttc tcgtagttct tgtaacgtaa atcgaaggtg tcaataaaag
    13381 ggacgcgttc aattttggca cgtaattctg caatttcttt gcgcagacgt tcctcttcca
    13441 gtagttgcgc aggttcactg ttgctgatga tggccaaatt ctcttccagt gcatgaagtt
    13501 cccgccgctt gcctgccgtc atggctgtgc gtcgcgccag tgagttctgc aaggaacgca
    13561 caacgctgat attggccgga acgccgttag cggtataacc cgcccgatgc gttttatatt
    13621 cggtcagctg gcgttgttgg ttttgtttca gattcggtaa ggccagatct tcaaagagca
    13681 gatcaagata ctcatctttc gagatctgaa agacaaattc atcctgacct tcaccatcct
    13741 ggctggcctg gccctgacca ctgccggaac ctccgccgcc accctgggga cgttcaattc
    13801 ggtcattctg gacgaaatgg tcattgcccg gatgcacgcg gtggcgcaga ccgccacgcc
    13861 cctgatgaaa catcggttcg ctaatatctt ccgtgggaat ggatacagac tcgccgctgt
    13921 cgacgtcagt caccgaacgc ttattaatgg cctcggagat cgactgttta atttgcgctt
    13981 tataacggcg taagaaacgc tggcgattca ccatgctttt gtttttgccg ttcagacgcc
    14041 ggtcaataaa ccaggtcata tgccccccgt actacatttg ccaactgtat gctacgcctg
    14101 tgttgccgct tctctggccc acggttcgct gtcgttgtag gcccggtagc atgtgtaccg
    14161 ggccagggtt gttaagacga tttacgtacg cgcaaatacc attcgcacag taaacgcacc
    14221 tgtttacggg tgtagccttt ttccatcata cggtcgacaa aatcgtcgtg tttcttttgc
    14281 tcgtcggttg acgttttggc gttaaacgaa ataaccggca acagctcctc ggtattggag
    14341 aacattttct tctcgataac cgtgcgcagt ttttcatagc tggtccagtt cggattgcgt
    14401 ccgctgttat tcgctctggc gcgcagtacg aagttaacaa tctcgttgcg gaaatctttt
    14461 ggattactga tccccgccgg tttctcgatt ttctccagct cggcgttaag agactcgcga
    14521 tcaaacagct gcccggtatc tggatcgcga tactcctgat cctgaatcca gaaatccgcg
    14581 taggtaacat aacggtcgaa aatgttttgc ccatattcgg aataggattc aaggtaggcc
    14641 gtctggatct ctttgccgat aaactcggca tatttcggga tcaagtaacc tttcaggaac
    14701 tccagatagc gttctgcctg ctcttgcggg aactgctcgc gctcgatctg ctgttccagg
    14761 acgtagaaca gatggaccgg gtttgccgcc acttctacat gatcgaagtt gaacacgcgg
    14821 gagaggatct taaacgcaaa acgcgtcgac agaccgttca tcccttcatc gacaccggcg
    14881 tagtcacgat attcctgata cgacttggct ttgggatctg tgtctttcag actttcgcca
    14941 tcataaaccc gcatctttga ataaatgctg gagttttctg gctctttaag gcgcgaaaga
    15001 atggaaaaac gtgacagcgt ttccagcgtg ccaggggcgc agggggcgtg agtcaattca
    15061 ctgtgattaa gcaatttctc gtagattttg atctcttcgg aaatgcgcaa gcaatacggc
    15121 accttcacga tgtaaacacg atcaaggaag gcttcgttgt ttttgttatt acggaaagtg
    15181 acccattcgg attcgttcga gtgggcgaga ataatcccgt tgaacggcag ggcggagata
    15241 ccttccgtcc cgttgtagtt accttcctga gtggcggtta gcaagggatg cagcacttta
    15301 atcggtgctt taaacatctc aacgaattcc atgatcccct ggttggcgcg gcacagtgca
    15361 ccggaatagc cgtaggcgtc cgggtcattc tgcgcgtagt gttcgagttt acgaatatcg
    15421 actttcccaa ccagcgcgga gatgtcctgg ttgttctcat caccgggttc cgttttggcg
    15481 atagcaattt gttgcagaat tgacggccag accttcacta cccggaactt agtgatatcg
    15541 ccaccaaatt catgcaggcg ttttgccgcc cacggcgaca tgatggtgcc gagataacgg
    15601 cgaggaatgc catactcttt ttccagaatc tgcgcatctt cctgcggatt gaaaagacag
    15661 aacggatgat cgttgaccgg gctacgctca ccgttggcgc tcaatacata aatcggtacg
    15721 agctgcatta atgatttcag tcgctcagca agcgatgatt tacccccacc cacaggcccc
    15781 agcagataca ggatttgttt cttctcttcc agcccctgag ccgcgtgttt cagataagag
    15841 acaatctgtt caatcgcgtc ttccatgccg taaaactctt caaatgccgg ataacgtgct
    15901 atgacccggt tagaaaagag tcgagaaagt ctgggttcct gggctgtatc gaccatgaca
    15961 ggctcaccga tagccatcaa tagccgctca gccgcgttgg cataagcact gcgatcttgc
    16021 cgacaagtgg taagaaactc ctgcagtgtg aactcttcgt ccttggcagc ttcatatcgc
    16081 tggcgatagt gatcgaatat attcatggta tgccgtcctt tcgtttttta gcacaggtta
    16141 agagccgttc atatgaatag tagaggctcc cggaagaggt aaactgaacg acgtctcttg
    16201 ctaaaacaca gcaactctca tgccatttta tcgacgatga gaacaacgtg cccggtgata
    16261 caccttcaaa attaagcgta gatggcaaat ggaaaacttg cctgcacggc tttcttaatt
    16321 tcaatggcat atcaacaact catccataaa ttttgcataa ttaatgtaaa gaccagggtc
    16381 gccagtaacg ctaaattcat ttggctgtaa gcgcggtgtc atccgcgtca ggaaaattaa
    16441 acagttactt taaaaaatga aaacgtaaaa aggttgggtt tcgatgtatt gacgggtaaa
    16501 ctttgtcgcc cgctaaacat ttgtttaagg aatgattaat tgtgaccaaa ctcaaacttc
    16561 tggcacttgg agtgcttatc gcaacgtctg caggcgtagc gcacgctgaa ggtaaatttt
    16621 ccctgggcgc aggcgtaggt gtcgttgaac acccatataa agattacgat accgatgttt
    16681 acccagtacc ggtaatcaac tatgaaggcg ataacttctg gttccgtggc ttaggtggtg
    16741 gttactacct gtggaatgac gcaacggaca aactttcaat taccgcttac tggtcgccgc
    16801 tttacttcaa agcgaaagac agtggcgatc accaaatgcg tcacctggat gaccgtaaga
    16861 gcaccatgat ggctggtctg tcttatgctc actttaccca gtacggttac ctgcgtacca
    16921 ccctggctgg cgataccctg gataacagca acggcatcgt ctgggatatg gcctggttgt
    16981 atcgttacac caacggtggc ctgaccgtga ccccgggtat cggtgtacag tggaacagcg
    17041 aaaaccagaa cgaatactat tatggcgtat cgcgcaaaga gtctgctcgc agcggtctgc
    17101 gtggctataa cccgaacgac agctggagcc cttacctgga gctgagcgcc agctacaact
    17161 tcctcggcga ctggagtgtt tacggtaccg cgcgctacac ccgtctgtct gatgaagtta
    17221 ctgacagccc aatggtggat aaatcctgga ctggcctgat ttctaccggg atcacctaca
    17281 aattctgata atgcatcttt ccagggcatt ttgtgcataa aaacagggcg tcatgccctg
    17341 ttttgcatta cggtggtgca atacctggga ggaaacaatg caacaaaaaa tgattcaatt
    17401 tagtggcgat gtctcactgc cagccatagg gcagggaaca tggtatatgg gcgaagatgc
    17461 cagtcagcgt aaaacagaag tagctgcact acgcgcgggc attgagttcg gtttaaccct
    17521 cattgatacc gccgaaatgt atgccgaggg cggtgccgaa atggtggttg gggaagcatt
    17581 aaccggccta cgtgataacg tctttctcgt ctctaaagtc tatccgtgga atgctggcgg
    17641 gcaaaaagcg ataaatgcat gcgaagccag tttacgccgt ctcaatactg attatctcga
    17701 tctctactta ttacactggt ctggcagttt cgcttttgaa gagactgtcg cagcgatgga
    17761 aaaattgatc gcccagggaa aaatccgccg ctggggcgtt tctaaccttg attatgccga
    17821 tatgcaggaa ctctggcagc tgccgggggg aaatcagtgt gccactaatc aggtacttta
    17881 ccatctcggt tcacgaggga ttgagtacga tctactcccc tggtgccagc aacagcagat
    17941 accggtgatg gcttacagtc cgttagctca ggccgggcgg ttgcgcaatg ggctgctaaa
    18001 aaacgcagta gtcaacgaaa ttgcacatgc tcacaatatc agcgcggcac aagtattgtt
    18061 ggcgtgggta atcagccatc agggggtgat ggcgattcca aaagcggcaa ctgtcgcaca
    18121 tgtccaacaa aatgcggctg tgcttgaggt tgaactttcg tcagcggaat tagctatgct
    18181 ggataaagcg tatccggcac caaaaggaaa aactgcgctg gatatggtgt gatgttgata
    18241 cgtgccggat gcatcgcacc cggcacacaa cattaacgca aattaacgtt tggcaacgcg
    18301 aatggattgc gccagatgtg caggtttttc tttggtgact ttttgcgttt ctgaagcgta
    18361 agccgtttct acacaaacaa aggtcttgta gccatcgtcc ggcatgtcgc ccatgctaac
    18421 tgaaagcgcc ggtcccggat tccagcctac aacgttcaga tgatgctggt gacctacggc
    18481 gataatacga ttcagcgctt catcattaat cacgctgcaa tcttgcggat tcagatacac
    18541 gcggtcggta cggtcaggga aggtctgaat accgtcggtc agtacatctt ctttcgcgtc
    18601 attcacttta tcaatgaagc gatcgcccag cccactgacg cttaccttag cgatatcacc
    18661 cacgttaaag taggtatgca gggcagaggt ggtttcaaat tcgccatgtg attcaagatc
    18721 gatttcacag gttttaccca cgcggaaatg cgctaacagc gtaaagtcgt gcggccagaa
    18781 ttttttcgtc tcttcgcttt gcgtcaattc aaaagtcagc gctacgccat cagcatcttc
    18841 atgatgtgat ttcagcgtcc acggcaggtt gcgggcaaaa ccgtgcgcag gcaggccttg
    18901 ttgtgcagcc ggaccaaacc acggccagca aaccggtacg ccaccgcgaa tagcgacgcc
    18961 atttttgaac ggtgtgttgt tactcaacca cagaacttct tcttcacccg caggtttcca
    19021 tgagagaagg tgtgcgccct gtaatgcaaa agaggctttt acctggggat gatcgaccac
    19081 aatgaggtcc agttcatcca gtttacgacg ggagaggaca ggggagattt gttcgatgac
    19141 cggaagggca aaaattttct taatcatgac gcagtccttt aacttcattt tatcaggtaa
    19201 aaaaaagagc gaccgaagtc gctcttttta gatcacagtg tcatctcaac ttatttggag
    19261 atgtgagcga tcaggtccag aactttgttg gagtaaccgg tttcgttgtc gtaccaggat
    19321 accagtttca cgaagttgtc gttcagagcg ataccagctt tagcatcgaa tacggaagtg
    19381 caaacttcgc cgttgaaatc ggtagatact acgtcatctt cggtgtagcc cagaacgcct
    19441 ttcatttcgc cttcagcagc agctttaacg gcagctttga tctgctcgta agttgcagct
    19501 ttttccagac gaacggtcag gtcaactacg gatacgttcg gggtcggaac gcggaacgcc
    19561 ataccagtca gtttgccatt cagttctggc agtactttac ctacagcttt agctgcaccg
    19621 gtagaggacg ggatgatgtt ctgggaagca ccgcggccgc cgcgccagtc tttgtgagac
    19681 ggaccatcaa cggttttctg agtagcggta gtagcgtgaa cggtggtcat cagaccttcg
    19741 atgatgccga agttatcgtt gataacttta gccagcggag ccaggcagtt ggtggtgcag
    19801 gaagcgttgg aaacgatgtc ctggccagca tatttgtcga agttagcgcc tttaacgaac
    19861 atcggagtgt tgtctttaga cggaccagtc ataaccactt tcttcgcacc agcggtgatg
    19921 tgtttacgag cagtttcgtc agtcaggaac agaccagttg cttcagcgac aacgtcaaca
    19981 ccaacttcgt cccatttcag gttagccgga tcacgttcag cggtaacacg gattttttta
    20041 ccgttaacga tcagatgacc gtctttcact tcaacggtac cgtcgaaacg gccgtgagtg
    20101 gagtcatatt tcagcatgta tgccatgtaa tcagcgtcta acaggtcgtt gattgcaacg
    20161 atctcgatgt cagaacgttt ctgagcagca cggaaaacaa tgcgaccgat acggccaaaa
    20221 ccgttgatac ctactttgat agtcatatat tccaccagct atttgttagt gaataaaagg
    20281 ttgcctgtaa aattacaaaa accttacgca gcgtcaagcg gaatcgtgtc aatcattgcg
    20341 acaaatcaat cctgtgccta agcattacgc gactgactcg cctcactctt cctttgggct
    20401 tgagaccaca tggggtcggc gtcccgaatt ttaaagggca attacgataa aaatgtgatt
    20461 ttcatcacga tttaggtgca gccatttcgt cgagatcaag tttaggacaa atttttgcca
    20521 gttggtgtta atgttttgtt agaatcagtc acgtaatgtg agcacgatta aagtgagatg
    20581 tgagcaaatg gctaataaac cttcggcaga agaactgaaa aaaaatttgt ccgagatgca
    20641 gttctacgtg acgcagaatc atgggacaga gccgccattt acgggtcgtt tactgcataa
    20701 caagcgtgac ggcgtatatc actgtttgat ctgcgatgcc ccgctgtttc attcccaaac
    20761 caagtatgat tccggctgtg gctggcccag tttctacgaa ccggtgagtg aagaatccat
    20821 tcgttatatc aaagacttgt cacatggaat gcagcgcata gaaattcgtt gcggtaactg
    20881 tgatgcccat ctggggcatg tcttccccga cgggccgcag ccaacgggtg aacgttattg
    20941 tgttaactca gcctctttac gctttaccga tggcgaaaac ggcgaagaaa tcaacggttg
    21001 aaaaaacgat tcagcaactt attccacagg agcggaaaaa ttatgaatct tgatgacatt
    21061 atcaacagca tgacgcttga ggtataccag cgtttgtcga ccgccgttga attgggcaaa
    21121 tggcctgatg gcgttgcgtt gaccgaggaa caaaaagaga actgcctgca actggtgatg
    21181 ctgtggcaag cccgcaataa taccgaagca cagcatatga caattgacac aaatggtcag
    21241 atggtcatga agagcaaaca acagttaaaa gaagattttg gtatctcagc aaaggctatt
    21301 gccatgttta agtaagtggt ttgtcccctt tttgccgcgg ttataaaggg gatagccaac
    21361 aataacccca cgactcatct caattatctc gtcacgacca ggctaagtcc cagcccggtc
    21421 tcttgcagtc gcgttcacgc ctgaatgact tctcctgaac ttaagtctca gacctatttg
    21481 gccggtaatc cctctcaaat ttgctcattt tgcgttccgc cttccatcaa cctgtaacag
    21541 atccagccat tttctgcgta attgtgatct gagttgtaaa acgcagatca ttatctgtgt
    21601 tttacagatt taatgttgac ctcaaaaatc atgtgggtaa gtatgatgac catctaagca
    21661 aaacaaaaca cagaggatga cgcaatgaaa gcactggctc ggtttggcaa agcctttggc
    21721 ggctacaaga tgatcgatgt cccacaaccc atttgtggcc cggaagatgt agtgattgaa
    21781 attaaagccg cggcaatctg cggcgcagac atgaagcact acaatgtcga tagcggttct
    21841 gatgagttta actctatccg cggccatgag ttcgcaggtt gtattgcgca ggttggtgaa
    21901 aaagtcaaag actggaaagt ggggcaacgc gtcgtgtcgg ataacagcgg tcacgtctgc
    21961 ggcgtttgtc cggcctgtga acaaggtgat tttctgtgtt gtacagaaaa agtaaacctt
    22021 ggtctggata acaatacctg gggcggtggt ttttccaaat attgtctggt tcctggtgaa
    22081 attctcaaaa ttcatcgtca tgcattgtgg gaaatccctg atggtgttga ttatgaggac
    22141 gcagccatac ttgaccctat ctgtaatgcc tataaatcca tcgcgcagca atcgaaattc
    22201 ctccctggtc aggatgtggt cgtcatcggc actggcccac tcgggctgtt ctccgtacaa
    22261 atggcgcgga ttatgggggc ggtaaatatc gtcgtcgttg gtctacaaga agatgtggcg
    22321 gtccgcttcc cggttgcaaa agaactgggt gcgacggcag tagtaaatgg ttctaccgaa
    22381 gatgtggtgg cgcgctgcca gcaaatttgt ggcaaagaca atctgggact ggtgattgaa
    22441 tgctccggtg ccaatatcgc actgaaacaa gccatcgaaa tgctccgtcc gaacggggaa
    22501 gtggtacgcg ttggaatggg cttcaaacct cttgatttct cgattaatga cattaccgcc
    22561 tggaacaaaa gcatcattgg gcatatggcc tatgactcca cctcatggcg taacgctctc
    22621 cggctattag ccagcggcgc tatcaaagtc aaaccgatga tcacgcatcg tatcggcctg
    22681 tcgcaatggc gcgaagggtt tgatgcgatg gtcgataaaa ccgcaatcaa agtgatcatg
    22741 acttacgact ttgatgaata actactctcc tgaaggaacg ttatcgcatg gaacagataa
    22801 caaaaccgca ttgtggtgca cggctggatc gcttaccgga ttgccgctgg cattcgtcaa
    22861 tgtttgccat cgtctcgttt ggcttgcttg tctgctggag taatgccgtt ggtggcttga
    22921 tcctcgcgca gctgaaagag ttgggctgga cagataattc caccactgcc acattctcag
    22981 caatcacgac cgccggaatg tttctcggtg cgctggttgg cggcatcatc ggtgacaaaa
    23041 ccggtcgcag aaatgcgttc atcctctatg aggccattca tattgcctcg atggtggtcg
    23101 gtgctttttc accgaatatg gatttcctta ttgcctgccg ttttgtgatg ggcgttgggc
    23161 tgggagcttt actggtgacg ctgtttgctg gtttcaccga atatatgccc ggtagaaatc
    23221 gcggaacgtg gtccagtcgg gtttctttta ttggcaactg gtcatatccg ctctgttcat
    23281 tgattgcgat gggactcacg ccgctgatta gtgcagagtg gaactggcga gtacaactgc
    23341 ttatccctgc aatattgtcg cttatcgcta cggcgctggc ctggcgctac tttcctgaat
    23401 ccccgcgctg gctggaatcg cgcggacggt atcaggaagc cgagaaagtc atgcggagta
    23461 tagaagaagg cgtcatacgc cagacgggaa aacctttgcc gcccgtggtt attgctgatg
    23521 acggtaaagc gccacaagcg gtgccgtatt cagccttaat gacaggagta ttactgaaac
    23581 gcgtgatatt aggctcttgt gtgctgattg ccatgaacgt tgtgcagtac acactaatta
    23641 actggttgcc aacaatattc atgacccagg ggattaattt aaaagactcg attgttttaa
    23701 ataccatgag tatgtttggc gcgccatttg gtatttttat tgctatgctg gtgatggata
    23761 aaattccgcg taaaacaatg ggtgtggggc tattaatcct gattgcggtg ctcggatata
    23821 tctattcact gcaaaccagt atgttgctca taacgttgat tggtttcttc ctgattactt
    23881 tcgtctatat gtacgtttgc tatgcctcgg cagtgtatgt ccctgagatc tggccgacag
    23941 aggccaaatt acgtggctcc ggtctggcga atgcagtagg gcgaatcagt ggtattgccg
    24001 caccttatgc cgttgcagtg ctgctcagta gttatggcgt aacgggagtc tttattcttc
    24061 tgggggcggt ttcaattatt gtcgcaattg ctatcgccac cattggaatt gaaaccaaag
    24121 gtgtctccgt tgaaagttta agtattgatg cagtagtcaa taaataatac tggtggattc
    24181 aaaatgaaaa attcaaaagc aatattgcag gtgccgggca caatgaaaat tatttcagca
    24241 gaaatacctg tgcctaaaga agatgaagtt ttgattaaag tggaatatgt cggtatttgt
    24301 ggttcagatg tacatggttt tgaatcaggc ccgtttattc cgcctaaaga cccaaatcaa
    24361 gaaattggct tgggtcatga atgcgccggg acggttgtgg ctgtgggaag ccgtgtgcgc
    24421 aaatttaaag cgggggatcg ggtaaatatc gaacctggcg ttccttgcgg tcactgtcgt
    24481 tactgtctgg aaggcaaata taacatctgc ccggacgttg attttatggc gacacaacct
    24541 aactaccgcg gcgcattaac gcactatctg tgtcatccgg agagctttac ttacaaactg
    24601 cccgacaata tggacatgat ggaaggggcg ctggtggagc ctgctgcagt cgggatgcat
    24661 gccgcgatgc tggcagatgt taaaccgggt aagaagataa ttattctggg agcgggttgt
    24721 attggtttga tgacgttgca agcgtgcaaa tgcctgggag ctacggatat tgccgtcgtt
    24781 gatgtactgg ataaacgtct gacaatggcg gaacagctcg gcgcgacagt ggttattaac
    24841 ggtgcaaaag aagacactat tgcacgctgt cagcaattta ccgaagaaat gggcgcagat
    24901 attgttttcg aaacagcggg ttctgcggtc accgttaaac aggcacctta tctggtaatg
    24961 cgcggcggta aaattatgat tgttggtact gtaccgggcg attcggcaat caatttcctc
    25021 aaaatcaatc gcgaagtcac tattcagaca gtattccgct atgccaatcg ttatccggtc
    25081 acgattgaag ctatttcctc agggcgattc gatgtgaaat cgatggtgac acatatttac
    25141 gattatcagg atgtacaaca ggcatttgaa gagtcggtta acaacaaacg cgacattatt
    25201 aaaggcgtta ttaaaattag cgattaatta atacgggaga acagaatatg ctcgcagata
    25261 tcagatattg ggaaaacgat gccacaaata aacattatgc aattgcccat tttaacgtat
    25321 ggaatgcaga aatgttgatg ggcgttatcg acgcagccga agaagcgaaa tcaccggtta
    25381 ttatttcttt tggtacaggt tttgtcggga acacctcttt tgaagatttc tctcacatga
    25441 tggtatcaat ggcaaaaaaa gcaacggtgc cggtaataac tcattgggat catggtcgga
    25501 gtatggaaat tattcataac gcctggactc atggtatgaa ttcattaatg cgtgatgctt
    25561 ccgcatttga tttcgaagaa aatattcgtt taaccaaaga ggctgttgat ttcttccatc
    25621 cgctgggtat tccggtagag gcagaattag ggcatgtcgg taatgaaacc gtttatgaag
    25681 aagcgctggc gggctatcac tataccgatc ctgaccaggc ggctgaattt gttgaacgta
    25741 cgggctgtga ctcattggct gtcgccatcg gcaaccaaca tggggtttat acgtcagagc
    25801 cacaattgaa ttttgaggtc gtcaaacgcg tacgcgatgc cgtttctgtt ccgctggttt
    25861 tgcacggcgc atcggggatc agtgataccg acattaaaac tgcaatttcg ttgggtatcg
    25921 cgaaaatcaa cattcatacg gagctctgtc aggccgcaat ggtagcagtg aaagaaaacc
    25981 aggatcagcc tttcctgcat ttagaacgtg aagtacgtaa agctgtaaaa gaacgggcat
    26041 tggaaaaaat taaactgttc ggttctgatg gcaaagcgga ataatgatat ggataatctc
    26101 gacgttattt gtataggtgc cgctattgtt gatattccat tgcaaccggt cagtaaaaat
    26161 atctttgatg tggattctta tcctcttgaa agaatcgcaa tgaccaccgg gggtgatgca
    26221 ataaatgaag caacaattat ttctcgtctg ggccatcgca cagcattaat gagtcgtatt
    26281 ggtaaagatg ccgccgggca atttattctc gaccattgcc gcaaagagaa tattgatatt
    26341 caaagcctga agcaggacgt cagcatagat acctcaatta acgttggact ggtgacggag
    26401 gatggcgagc ggacgtttgt cacaaactgt aacggcagtc tgtggaaatt gaatattgac
    26461 gatgttgatt tttcgcggtt ttctcaggcg aaattattat ctctggccag tattttcaac
    26521 agtcctctat tggatggtaa agcgctaaca gaaattttta cgcaagctaa agcccggcag
    26581 atgattatct gtgccgatat gatcaaaccg cggttgaatg aaacgctgga tgatatttgc
    26641 gaagcattga gttatgtcga ttatctgttt cctaattatg ccgaggcaaa attactcacc
    26701 gggaaagaga cactggatga aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg
    26761 gtgattaaaa cgggtaaaga cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg
    26821 ccggcggtcg caggaataac cgccatcgac accattggcg cgggcgataa ctttgcttca
    26881 ggttttattg cggcactgtt agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat
    26941 gcaacggcgg ctatctcggt tctaagcgtc ggtgcctcca ccggcgtaaa aaacagaaag
    27001 ctggtcgaac aattgctgga agaatgcgaa ggataatgaa ggcaaatgaa aaagatacct
    27061 ttaggcacaa cggatattac gctttcgcga atggggttgg ggacatgggc cattggcggc
    27121 ggtcctgcat ggaatggcga tctcgatcga caaatatgta ttgatacgat tcttgaagcc
    27181 catcgttgcg gcattaacct gattgatact gcgccaggat ataactttgg caatagtgaa
    27241 gttatcgtcg gtcaggcgtt aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa
    27301 tgcggcattg tctgggaacg aaaagggagt ttattcaaca aagttggcga tcggcagttg
    27361 tataaaaacc tttccccgga atctatccgc aaagaggtag aagccagctt gcaacgtctg
    27421 ggtattgatt acatcgatat ctacatgacg cactggcagt cggtgccgcc attttttacg
    27481 ccgatcgctg aaactgtcgc agtgctcaat gagttaaagg acgaagggaa aattcgcgct
    27541 ataggcgctg ctaacgtcga tgctgaccat atccgcgagt atctgcaata tggtgaactg
    27601 gatattattc aggcgaaata cagtatcctc gaccgggcaa tggaaagcga actgctgccg
    27661 ctatgtcgtg ataatggcat tgtggtacag gtttattcgc cgttagagca gggattgttg
    27721 accggtacca tcactcgtga ttacgttccg ggcggcgctc gggcaaataa agtctggttc
    27781 cagcgtgaaa acatgctgaa agtgattgat atgcttgaac agtggcagcc actctgtgct
    27841 cgttatcagt gcacaattcc cactctggca ctggcgtgga tattaaaaca gagtgattta
    27901 atctccattc ttagtggggc tactgcaccg gaacaggtac gcgaaaatgt cgcggcattg
    27961 aatatcaact tatcggatgc agacgcaaca ttgatgaggg aaatggcaga ggccctggag
    28021 cgttaaatac tcaacacgat tcacctctgt ttctgtgaaa actgtgatga aaatctgtga
    28081 ttcaaacagg ttattttgaa agtaaacatc ggttatgcga taatcgcgct aatgtgatgt
    28141 aaaaagatac aggggtgtaa tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat
    28201 ggttgccaac gataaaaagg tgacagtctc aaatttgagt gggatttttc aggtaaccga
    28261 agaaaccatt cgccgcgatc tcgagaagct ggaagatgaa ggctttttga ccagaaccta
    28321 tggtggtgct gttttaaata cagcgatgtt gacggagaat atccattttt ataagcgcgc
    28381 ttcatcgttt tatgaagaga agcagcttat tgcacgcaag gcactaccct ttatcgacaa
    28441 taaaaccact atggcagccg attccagtag taccgttatg gaattgctca aattgttgca
    28501 ggaccgtagt gacctgacgt tgctaactaa ctcggcggaa gcgattcatg ttctggctca
    28561 atcagaaatt aaagtcgttt caacgggggg ggaactaaac aaaaatacac tttccctgca
    28621 aggaagaatt actaaagaga tcatcagccg ctaccatgtc gatatcatgg taatgagttg
    28681 taaaggtctt gatattaaca gcggcgcgct ggactctaac gaagcggaag ctgaaatcaa
    28741 aaagacaatg atccgtcagg cgacagaggt tgcgttattg gttgatcact ctaagtttga
    28801 tcgcaaagct tttgtccagt tagctgactt tagtcatatt aattacataa taactgataa
    28861 atcaccgggt gcagagtgga tagcattttg caaagacaat aatatccagc tggtgtggta
    28921 atttaagcac acgttgtcaa tattatttgt tcctgcgcct ggaaaataaa atgatgataa
    28981 agatgcggca agtgaatttg ccgcaattgc taccttttta gcaaatccga ggcacctaat
    29041 ggaacaatat gatcaaattg gcgcaagact ggaccgcttg cctttggccc ggtttcatta
    29101 tcgtatattt ggtattataa gctttagtct gttattaacg ggttttttga gttattcagg
    29161 caatgttgtt ttagcaaagc tggtaagcaa tggatggtca aataatttcc tcaatgccgc
    29221 ctttacctcg gcattaatgt ttggttattt catcggctct cttaccggtg ggttcatcgg
    29281 tgattatttt gggcggcgca gggcttttcg cataaatctt ctcatcgtcg gtattgcggc
    29341 aacaggggcc gcttttgtcc ctgatatgta ctggctcatc ttctttcgct tcctgatggg
    29401 cacaggaatg ggggcgctga ttatggttgg ctatgcttcg tttacggagt ttatccccgc
    29461 gacggtgcgt ggaaaatggt ccgcgcggct ctcatttgtc ggtaactggt cgcccatgtt
    29521 gtcggcggcg ataggcgtgg tggttatcgc tttttttagt tggcggataa tgtttctgct
    29581 gggtggtatt gtcatactgt tagcctggtt tctctcaggt aaatacttta ttgagtcgcc
    29641 tcgatggttg gcggggaaag gacaaatcgc aggtgcagaa agccaacttc gtgaagtaga
    29701 gcagcaaatt gaaagagaga agagtgttcg tttacccccg cttactttga atcagagcaa
    29761 cagcaaggct aacgtaatca atggtacttt ctggctgctg tttaaagggg aaatgttacg
    29821 acgtacatta gtcgcgatta ctgttttaat tgcaatgaat atttcacttt ataccatcac
    29881 cgtatggata ccgaccatat ttgttaactc cggcattgat gtcgataaat caatattaat
    29941 gactgctgtt attatgattg gcgctccggt aggaatattt attgcggcat taattattga
    30001 tcattttcct cgtcgattat ttggctccgc cttacttatt attattgccg tgttaggcta
    30061 tatctattca attcagacta cagagtgggc gattttaatc tatggcctgg tgatgatctt
    30121 ctttttatac atgtatgttt gcttcgcgtc ggcggtttat atcccggagc tctggccaac
    30181 gcatttacgc ttgcgcggtt cgggtttcgt taatgccgtc ggacggatcg tcgcggtctt
    30241 cacgccttat ggcgttgcgg cactattaac gcattatggg tcaatcacgg tgtttatggt
    30301 gcttggcgtg atgttagtgc tctgtgcgct ggttctctcc atttttggca tcgaaacgcg
    30361 gaaggtgtcg ctggaagaga tttctgaggt gaattaaatc tttcgttgat ggcgtcaatt
    30421 gccagatgta ccactgacgt gttttatctg gcctacaatg gcagtacaga attgtaggcc
    30481 agcataaaat tactcctgcg tctcttccca gtctgccagc gtatatagcg ttgccccagc
    30541 tgctgacatc tccataaacg cgtgcgcact gtcctgtggc tggatattca caccacgaca
    30601 accatcggta atcacattaa ccttataacc taactgtaac gcgtccagta cggtaaactt
    30661 cacgcaatag tcagtagcca ggcccataat gatcaattca tcgatttcat gatcgcgtaa
    30721 ccagtcatcg agcgtggttt tctgccgacg gccgttatca aaaaaggcac tgtaactgtc
    30781 aactaaagga ttttcgcctt tatggaacac cgctgcgatc gctttttggt tcagtaacgg
    30841 atgtaattgt gcgccttcac tgttctgcac acagtgatct ggccagaagg tttgtggcaa
    30901 accgtcgagt tggcctggcg tataaggctc tacaccgtgc tgactggcaa aactgccgtg
    30961 attcgccggg tgccagtcct gactggcgat aaccgcttca ccgcgcgact ggcaccagtc
    31021 aatcaggcgg ttagcgacat ccaccgtact gtcaccttcc ggcacggcga gcgcgccacc
    31081 agcacagaaa tcattttgta aatcgaccag taacagggcg cgagggggca ttgcagtctc
    31141 cttaatcatc cggcgtcagt tcgccgcgca ggttttggct catggccttg cgaatggttt
    31201 cagtatccag ttcctggcta agcaggtaat gcagtttggt tagtgtggct tctacagtca
    31261 tatctgcacc gccaataacg ccggcatggg cgagggcgtt accggtggca taaccaccca
    31321 tgttcacttt accggacata cattgtgtca ggttgaccac cacaataccg cgatcgctgg
    31381 cttcttgtaa ttcctgcagg aaggctttgt tttgtggcgc attacccacg ccatatgagc
    31441 gcagaatcaa tgctttcacc ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc
    31501 ctggataaat cgtcactacg ccaatcggtt gtggggtgat tggatgaacg atcaattccc
    31561 cttcaccgtg cggggcgggt ggcgtattca aacgacgaat atggatacct gcttccagta
    31621 acggaggaag gtttggagag gcaaacgcat caaaaccatc ggcatgggct ttggtggtac
    31681 ggttgccacg ataaagtcga ttattaaaaa agagagttac ttcgttgata ggataattcg
    31741 ccgcaacgta caacgcattc agcagattaa tttgtccgtc agagcgtaac tcagctagcg
    31801 ggatttgtga ccctgtcaca atgaccggtt taccgagatt ctcgagcatg aacgacagcg
    31861 cagaggcggt atacgccatc gtgtcggtgc cgtgcagaat gacaaaacca tcatagtcgt
    31921 cataatgcgc tttaatatct tcagcaatat gctgccagtc ttctggcgtc atatctgaag
    31981 agtccatcag cggcgtatat tcatgaatgg tgaaatctgg catctccggg cgatggaatt
    32041 ccggcatcag cgccagttgg cgctgtagat gacctgacac cggaatataa ccctgctcgg
    32101 aacgctgcat cccgatggtc ccgcccgtgt aggcgacgta aattgatttc ttttgcatga
    32161 tattgatatg aatgatatcg aaagagcaaa agtatatacg tgaggcaggg ggagcgcacc
    32221 tgatactatg cggataagga aatatggggc gagacagcgg gaagaggatt cgtcgaggta
    32281 aaaaaatgtg gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca
    32341 ggtcaggcaa aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc
    32401 acttttaacg gtagaggcta ccgaggccag cggtgcaggt aacatggcct ggaacgcatc
    32461 tggcaacatt gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg
    32521 ttcatcaacg tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc
    32581 gaccgcatca tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc
    32641 ggtccagacg tggccctggg cgattttatc aatctgctcc ggcgtcgaat gacgcgcatc
    32701 agcaaccagc gtgataaagc gtttatagcc attctcaatg cttaactgca tcatctgctg
    32761 cgcttccggc ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc
    32821 atcagtatga acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat
    32881 accgatagaa ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat
    32941 ccagtaacca ccagatgctg ccatgccacc catcgataca accacaggct tacccgctgc
    33001 ccgggctgct gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct
    33061 attaacacgc aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc
    33121 cgcagtggta tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt
    33181 agcgaagacg acaccgatac tgtcaccggt atctgccggc gttttcaatg cgtaatcgta
    33241 ataactgatg gcgcgataat ttttatcagt tttactccag ccgaactctt tggtcagagt
    33301 tttttcgatt tcggcactcg atgccagtgc atcgaccagc ttgttttcca gcgcatattt
    33361 cgcggtatcg ccaccggttt tggttaaacc ctcaagcacc ccttgcgcgc cagggaatac
    33421 ctgctgagca gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc
    33481 accaatccag cggctgtcag cttcgcgggc tgcaggcgac atatcatcac gaataaacgg
    33541 ttcaacggca gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc
    33601 cagcaacgat ttgtagtaca gaccgttggt ggcaaagccg tgcagatcca ccacgccttg
    33661 cggtgacagc caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt
    33721 ctcgccaacg gcataaaccg gtttcccgct gtcacgaaac tctttcagag ctttgccgat
    33781 gtactgcata gacggctggt cgccacctgc gaagtttttc agatccatca cgataccggt
    33841 gatattgcgg tcatccttcg cctggcgaat ggtattgacg atatcaaaca gtgagttttc
    33901 ctgcagacga tcggaactgg caccaagcag ttggcggctt aatttactaa accgctgaga
    33961 actgtcgggt ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc
    34021 cgtttctttc gaatcaccac cactgacctg catccaaatc cccacaccaa ccagtacgag
    34081 aaaaataaag aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca
    34141 tttaaaaaat ccggcaataa atcgccaaag ggttcgcatg tattctccca actaaaggtc
    34201 acacctgtca caggcgcaat agatgtggtt atcgtaatga cccaaccggc acttgtcagc
    34261 aggaattgcg gactgggctg taacaaaatc cactctcgtg ttaattttgt gaataaatat
    34321 cacgacagga gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc
    34381 ccgcctggct gaacctgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat
    34441 gcgtgcgccg gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg
    34501 gcgcgagcgt ttcagcgccg ttctggagca gggggcgatt gctggcggta gtgatgacaa
    34561 agccatcgac aaagcccgta atgcgccgta ccgcgcaccg ctcatcataa cggtggtggc
    34621 aaaatgcgaa gaaaatcata aagtcccgcg ctgggaacag gaaatgtctg cgggatgcgc
    34681 ggtcatggcg atgcaaatgg cagcagttgc ccaggggttt ggcggcatct ggcgcagtgg
    34741 cgcattaact gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat
    34801 tgtcggtttt ctctaccttg gtacgccgca gctgaaagca tctacgtcga ttaacgtccc
    34861 ggacccgacg ccgtttgtca cttatttctg ataattgtcg cgaaactgtc tggattgtga
    34921 gcaaaggcgc aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata
    34981 cccggattgc attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac
    35041 agccacggag ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac
    35101 agtgagcagg cgaagtttgt tgatccgaat ttgcttgtgg gtaatgaaac ccgcgacgat
    35161 gcggcggtgt acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg
    35221 ccgatcgttg ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat
    35281 atcttcgcga tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac
    35341 aaactttccc cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag
    35401 gcgggtattg cgctggctgg cggtcactcc atcgatgcgc cggagccgat ttttggtctg
    35461 gcggtaacgg ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga
    35521 tgcaaactgt tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa
    35581 tcactgctga aaccagaaca tcagggactg gcgacggaag tgatgtgccg gatgaacatc
    35641 gcaggcgcgt cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggcttt
    35701 ggtctgctgg ggcatttaag cgaaatgtgt cagggtgctg gtgtgcaggc gcgcgtcgac
    35761 tatgaagcga tcccgaaact gccgggggtt gaagagtaca ttaagttggg cgcagtgcct
    35821 ggcggcaccg aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa
    35881 gtgcgcgatc tgctgtgcga tccgcaaact tctggcggtc tgctgctggc ggtcatgccg
    35941 gaagcagaaa atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcgatt
    36001 ggcgaactgg tgccagcgcg cggcggtcgt gccatggttg agatccgtta attcaatgcg
    36061 gttgtttatt gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc
    36121 gcaccggaaa ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat
    36181 cggtcacctg cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa
    36241 tcttgctgat ttgccgattg tcccggaaaa gtggcaatta cagccccgac cctccgtgac
    36301 caaacaactt aatgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg
    36361 tgacccggat cgcgaagggc aattactggt ggatgaagtg ctggactatc tgcaactggc
    36421 accggaaaag cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt
    36481 tgagcgggcg atcgaccgtc ttcgttccaa cagtgagttt gtgccgttgt gcgtttctgc
    36541 actggcgcga gcccgagccg actggctgta cggcatcaat atgacccgcg cttacaccat
    36601 tctcggtcgc aatgcgggtt atcagggcgt actttccgtg ggacgcgtgc agacgcccgt
    36661 actcggactg gtggtgcgcc gcgacgaaga gattgaaaac ttcgtggcga aagatttctt
    36721 tgaagtcaaa gcgcatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc
    36781 gagcgaagcg tgtgaacctt atcaggatga agaagggcgc ttgttacatc gtccgctggc
    36841 ggagcatgtg gtcaaccgca tcagcggcca accggctatt gtcaccagct ataacgataa
    36901 acgggaatca gaatccgcac cgctgccgtt ttcgctttcg gcgttgcaga ttgaagcggc
    36961 aaaacgcttt ggtctgagtg cgcagaacgt gcttgatatc tgccagaagt tgtacgaaac
    37021 gcacaagcta atcacttatc cgcgttccga ttgtcgctat ctgccggaag agcattttgc
    37081 cggacgccac gcagtgatga atgccatcag cgttcatgca ccggacttgt taccgcagcc
    37141 agtggtcgat ccagatatac gcaaccgctg ctgggatgac aaaaaggtcg atgcgcacca
    37201 cgccatcatc ccgaccgcac ggagttctgc gatcaacctg acggagaacg aagcgaaggt
    37261 ctataacctg attgcccgtc agtatttgat gcagttctgc ccggatgcgg tgttccgcaa
    37321 gtgtgttatc gaactggata ttgccaaagg caaatttgtc gctaaagcgc gttttcttgc
    37381 tgaagcaggc tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggtac
    37441 gccattgcct gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga
    37501 gcggcaaacc cagccgccgc gtcattttac cgatgcaaca ctgctttcgg cgatgaccgg
    37561 gatagcacgt tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct
    37621 ggggacagaa gcgactcgcg ccgggattat tgaactgctg ttcaagcgag gcttcctgac
    37681 caaaaaaggg cgctatatcc actccaccga cgccggaaaa gcattattcc attcgctgcc
    37741 agagatggcg acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag
    37801 cgaaaagcag tgtcgctacc aggactttat gcaaccgctg gtggggacat tgtaccagtt
    37861 gattgatcag gcaaagcgca cgtcggtaag gcagtttcgg ggaataatgg ctcccggcgg
    37921 tggagaaggg aagaaaaagg attcgccacg caagagagcg ccgaaaaaaa gcccgccatc
    37981 agaagaggcg ggcaatggag tgataacata aaggttcttt caaccatctg atactgaaga
    38041 tttttatggc cggattaggc gtttgcgctg tatccggcac aattgtctta aatcacgccc
    38101 tggcttaaca tcgcatccgc aaccttcaca aaaccggcaa tgttcgcgcc ctgcacgtag
    38161 ttggtttgct caccttcacc accatgctca acacaggcat ggtggatatc cagcatgatg
    38221 tgatgcaaac gtgcgtcaac tttctcggct ttccagccca ggcgcgcagc gttttgcgcc
    38281 atttccaggc ccgatgttgc tacaccgcca gcattagccg ctttacccgg tgcaaacagt
    38341 acgcctgcct gctggaacag ttcagtcgct tcgatggtgg tcggcatatt tgccccttcg
    38401 gcgacggctt taacgccatt ggcgataagc tgatgcgcgg cgtcaacatc cagttcgttc
    38461 tgggtggcgc aaggcagggc gatatcaact ggtacagacc acggctgttg gccttcgaga
    38521 tagaccagac caaattcttt ggcgtaatct gccactcgac catcgcggct ggctttgatt
    38581 tcgataagac gcgccagttt ctctttcgtg aatccgcttt catcaactac agtgccactg
    38641 gagtctgacg cagtgatcac acgagcacca aattccatcg ctttttcgat agcgtactgg
    38701 gcgacgttgc cggagccaga aacggaaacg cgcattcctt caaaacccat accgtggcgt
    38761 tttagcattg cttctgtgaa ataaaccaga ccgtagccgg tagcttccgg gcgaataaga
    38821 ctgccgccaa atgaaaggcc cttaccggtg aagacgcagg cggtattgtt ggagagcttt
    38881 ttcatcatcc ccgccataaa gccgacttca cgaccaccaa ccccgatatc acctgccgga
    38941 acgtcggtat ccgcgcccag gtggcgatac agttcagtca tcagcgcctg gcaaaaacgc
    39001 atcacttcac cttcgctttt tcctttcgga tcgaaatcgc tgccgccttt accaccgccc
    39061 atcggcagag tagtcagggc atttttgaag gtttgttcga agccgaggaa tttgagaatg
    39121 gaaaggttaa ctgacggatg gaagcgcata ccgcctttgt acgggccgat ggcagagctg
    39181 aactgcacac gccatgcacg gttgacctgt acctggttgc gatcatcaac ccataccacg
    39241 cgaaactgga tcacgcgctc cggttcaacc agacgctcca gtaatgacat ctggcgatat
    39301 tttggatttt gttcaagaaa aggccagagt gtggtcatta cttcacgaac ggcttgcgcg
    39361 aactcggttt gattcgggtc gcgcttttgg acatggttga ggaatgactc cagagaatat
    39421 gtctgatcca tagatataaa acccttatat attaatacga ttgtgcttat gttgtttatg
    39481 tcatgtgctt ttgcagtttt cgactatacc atcaagaatg tgacggtaag caagaaaaaa
    39541 atgacccagg aaagcaaatt aataacgaga gtaatctcat aaccaaatgc gataactcag
    39601 acgctaaagt tttgccaccc ggctaccgtt atcagtagca gagacaataa cgttaaggag
    39661 ttccctgtga gtcgagcatt gttcgccgta gtattagcat ttccattaat tgcactggct
    39721 aacccgcatt atcgtccgga cgtggaggta aacgtaccgc cagaagtgtt cagttcaggc
    39781 gggcagtcag cgcagccctg tactcagtgc tgcgtttatc aggatcaaaa ttattctgaa
    39841 ggggcggtaa ttaaagccga aggaatactg ctgcaatgcc agcgtgatga caaaaccctt
    39901 agcactaatc cgctggtctg gcggcgcgta aagccataaa cgcctctaat aatggaatgt
    39961 cagcaggggc cagcggatat tgcagcgcct cttcaggtga gcaccagacc agcgcctgat
    40021 gttcatgtgc ctgtaacgtc ccgtggaagt cgggtacgtg ccaggcatga agatggataa
    40081 tccgccccga aacttctcgc tgatggctgg caacatattc acccacagct gcttcgatgc
    40141 ccagttcttc gcgtaactca cgcaccagcg cctgccgctg gctttcatcc ggctcgactt
    40201 taccaccggc aaactcccat aatcccgcct gatcgctatg ggcggggcgt tgcgcgagta
    40261 aaattttgcc atcacgttcg atgatggcgg caacaacttc aaccattttc ataaattaca
    40321 cacgggtaga taagcgaaaa atgccataat atccgcactg ttgctcaatc agaaaggctt
    40381 tatatttaag gagtgcctgt gctagaccgc catcttcatc cccgaattaa acctttgttg
    40441 catcagtgtg tgaaggtgct ggataaaccc ggcatcacgc cagatggttt aacattagtg
    40501 ggatttgcca tcggtgtgct ggcactgcct tttctggcgc tgggctggta tctggcggcg
    40561 cttgtcgtca ttttgttaaa caggctgctt gatggtctgg acggtgcgct ggcgcggcgc
    40621 agagggctta ccgacgcggg cggctttctc gatatttctc tcgattttct cttttacgcg
    40681 ctggtgccgt ttggttttat tcttgctgca ccggagcaaa atgcgctggc aggcggctgg
    40741 ctgctgtttg cgtttatcgg tacgggcagc agttttctcg cctttgctgc actggcagca
    40801 aaacatcaga ttgataaccc cggttatgca cataagtcgt tttactatct ggggggatta
    40861 accgaaggca ctgaaacgat cttactgttt gtgctgggat gtttattccc tgcatggttt
    40921 gtgtggtttg cgtggatctt cggtgcgttg tgctggatga cgacctttac gcgggtgtgg
    40981 agtgggtatc tgacgctgaa gtcgctccag cgtcagtaat tgcaatgtta tttgctgctg
    41041 tccgggccgc gttcaccggt tgcaaccgga ttttttggat cgctgctcca ttcgtaccag
    41101 ccgccgtcgt aaacggagac gttattccag cccattgctc gtgcgtacat aaaggtttcg
    41161 gatgcgcgcc agccggtgcc gcagtagaat gaaacttgct gatctggctt gatattccat
    41221 gctttccaca tagcggtaat atcatcggcg ctacgcatgg tgccatccgg gttatggaaa
    41281 tcttccatat gcgtcgagtc gctaccagcg tgtccccaac gtgctccggc tatttcacct
    41341 tttggtttaa tatagctgta accgctggtc gtaccgataa attctggcca cgaacgaatg
    41401 ctcaccagcg atgcatcctg gcgatgcagc agtccacgcg cctgttccat atcaagcatc
    41461 agttgcggtt gtgccgggat cttcacgccg aaatccggtt ccgctttcac tttcggtggc
    41521 gttccgcgct caacaggcag tcccgcgtcg gaccaggttt gccagccgcc atccagcagg
    41581 cggacatctt tcacgccggc ataaagcatt atctgcgcca cacgcgctgc agcgtatacg
    41641 tcacgcccgt acagaatgac cgtggtgtca tggcgaatgc cgtgttttgc cagcatcgct
    41701 tttagttgtt catcagaaac tttgttccac agcggttcgc tttccacttc gttggtatcg
    41761 atgtagtcag cgccgggaat atgactgata aggtaaagct taggagcgcc ccaggccgct
    41821 tcaatgactt tccagtcacc ggcaggtttc gccgtaacct ctttaccttg ttgcaggtcg
    41881 tgcagccatt gcggataaac cagctgctca aagtgcggca gtttttgcag acgggaaggt
    41941 tcacttagcg cgtcactcag gatggagata tgcgtaaaac ctgctttttg cagacgcgtt
    42001 ttgacggcat cgacatcttt gtcattaccg tacagcgcaa ccggagcatc ggctttcagg
    42061 ttatgtagct tgatccacgc gttaagctgt tcggtgctca ttttgtcgag ccagctggca
    42121 gagaggttta aggcggcagg ctcatgacca gaagggccat ttaagatttg tggccagccg
    42181 ttataaaacg cgctggggcg agtatctatc gctttgccat tttgttgttg aagctggtca
    42241 agtgtaagag gcttcgccag ttcagcggcc cacgaagaag cgcaagccag ccctaaagcc
    42301 attgccagcg cggtcatttg agaaacacgt ttcatcgaaa atcccgacat tgtagttttg
    42361 agtgcgccat tctgaactga cgcattggga aaaactttgc gttatcgcag tttgttgtaa
    42421 ttttctgacc actgcgccat atccagaaca gaactatcag caggaacatc ctggagatcg
    42481 tgcgttacct gaacgactgg gatcgcctgg gcgcgaactt cgctgaacac ccactggcga
    42541 aaattatcgc gcagagctac atcaagacgg ctgaatggct catccagtag caacgctttt
    42601 ggctgagcga gaagagcgcg aagcagagca acgcgcgctc gctgaccgcc agacaaagtg
    42661 gcaggatcct gatggaacat accgccaagc cccgcacgct ccagcgcatc atttaccgca
    42721 tcgcgccttg caacaccttt gagtgccgct ggcagagcca gcagtaaatt ttgcccgacg
    42781 ctgaactggt caaataacag tgcatcctgg aaaagaatgc caatctgacg ctctgcggtg
    42841 ggtagcatgt caatccgttg ctcattgagc catagctcac ctgtacaaga aaactgtccg
    42901 gccagcgcac caatcatcca tgaaaacaga gtggattttc cacagccaga cggccccatt
    42961 aacgtgacaa tgtcacattt atccaccgta aagttaacgt ttgtcagcaa gcagctttct
    43021 ggtaaacgta gcgaaacatt tttcacgcag agcattagcg gagtccttgt ctgacataac
    43081 ctacccattt tgcgactaac gcggtcaggg caaaaataat aagcggcaac agtagttgcc
    43141 ataaagcctg ggcggcgaga ataccgttgc tgccgccgct gcttaatgcc accgcttcag
    43201 tggtgagcgt cggaaaacgc cccgcgccca gccacagcgt cggcatatac tgtgcaatac
    43261 tgactgaaaa tcccaccgcg aagacaatca gcgcagggcg tagcatgagt gggcatttca
    43321 cgtaaaagaa gattttggcc cgcgaccagc ccagtgtttg cgcaatcaaa attaaccgtg
    43381 aatcaatgcg ctgccaggcg ggttgcagga taaacagcat ccacggcatt acccacagca
    43441 gatgccccca gaccaccgcc gtccagcttc catccaggtt cagccatagc gccagcgtgt
    43501 actggcccgc caccagcggc agagcgggta ataaaatggg caaccatagc cacaactggc
    43561 gacgctgtgg cccccattcc agccacagca gtaacaggag caaagcgatg aatgccgcca
    43621 ccagccccat tgtcaggctg ttgatgagcg cttcactatt gatcgtcgac tgatccgcga
    43681 ggatcgccag cagaaccaca cacagcacac cggttaaggg taaaaaactc gccagcgtat
    43741 tgcctggcaa taaaggcgtg gcaggcttac gaacgccatc tacgcgggga atagtacgcc
    43801 gccagctacg ccatagcaga tagcccagca aaacgtaggc ggcgagtaac agcatcagca
    43861 gcaggctggc gagtgcgcct ttcgtttgtt gatcggcgtc gccctgagtt aaccactgcc
    43921 agctaattac tgccagcgtc gggggattac ctggcccaag gataatcgcc acatccacga
    43981 ccgacagcga ccaggcgaca atcgccagca tcgccattgc cagtgcaggt gcgacggagg
    44041 gcaacaacag ccagttcagg cattgccagc ggctgtagcc cagcgaatcc agtacaataa
    44101 cttgctgcaa cagccgtttt tcgctcaata ctgccgctaa gatccacagc agaaatgcgc
    44161 tttctttcac tgcaagggtg aggcccagac cgatgccaaa tcggtccatt ggcggagtga
    44221 aatacgggaa atagtcataa agcagccctc cgtcagcaaa gagcagaagg gcgctggtgg
    44281 caaaagccac atggggaatg gcgagcagcc acggcagacg ggcgcacata cgctgccatt
    44341 tcggcccagg ccacagcgcc acaatcacca gcagggcaat caacaacgcc ccgaccgccg
    44401 cgatggttgt cgacaccagc gttgccagta atgcctgcgg taactgtgga tcggcaaata
    44461 acgtctgcca gtgtgtcaac gacaaagcag gcgatatcag cgtgagagct gccgggatca
    44521 gcggtgcata aatcaccgcc accatcgccc acagcaaaaa gattaatgcg taccgtaacg
    44581 gtgtagccat tcttgttcca gcgcatttac ccaacctgcg tgcggttcag ccagtaccgg
    44641 cggcagatcc tgcggcattc ttgattgcaa tatttcgcgc tgcccgtcag gcagtttttg
    44701 cggatcgaga accgaaggat cgccccagac agcagggtct gctttacgca gttgtgcatc
    44761 gggtgagagc aggaaattgg caactacctt cgccgcagca ctggcattcg cgttggcggg
    44821 aatggtgacg aaatgcacgt tgccaatcat tccctcgcta aagccaaagc tgtaactgct
    44881 ggcgggtaaa tcgccgctgg cgattttttg ctgcgcatgc gcgggattaa aggtcagcga
    44941 caggcgcaat gtgccggctt tcagcagagc atccatccgc gcgggcgaag gcgggaaatc
    45001 ctttccttcg cgccacaaat acggatgcag cgcatcaaga tattgccaca agggagcagt
    45061 gacacgggcg aaagtcgcat cgtccggcgc ttcttttaat gctgtgggat ctggcgttag
    45121 cataatcagc aactgttcaa gaaacgccgt gccggtaaag tccggtgggc gcggataggt
    45181 aaccgtaccg ggattggctt tagcaaactc cagtaaggct tgcggcgttt gtggtggctg
    45241 cggcgtaaca tcgcggcggg cgataaacgt cagttgcgcg cccccccagg gcgattcagc
    45301 cccttctgta ggcactgaaa aatcttcccg caccggcagc tgtgtgtcga catagcgcca
    45361 gttgggcaga gtctccgccc agcccgtttg taataaattg gcctctttta aggtgcggaa
    45421 gttttcgccg ttcacccaga gcagatccac cgagccgcct gttttgcgtc cggcagcagc
    45481 ttcggtctga atgcgcttca ccgcgtctgc ggcatccgcg agacgaacaa tcttcaggtt
    45541 tatagcgtaa tgggttttca tctcgccgct aacccagtcg agatagcggt taattgcggt
    45601 atcgccgccc caggcgttaa accagacggt ttgtcctttg gcctcatttt ttatttcctg
    45661 ccagtcactg gcatgtgttg ccaaagaaaa cagcgataac aatcccagca accacccaca
    45721 atggcgcatc atctctcctt aagaacgttt ttcaggaata cggcacagcc cctgagcggg
    45781 aatgtccatg gcagaattaa aacgggctga caggttttgc aggccgatca atgccgtcag
    45841 ttcggtgagc gcctgggcgt caaaatgcgc agccagtcgg gtacgcaggg catcatcgac
    45901 cgttggcggc gttacgcttg cggcttcggc gtactccagc gccagccgtt cttcatcgct
    45961 aaagagcggg ctttgccgcc agtcagccac tgccagcagt ttatcggtgc tgccagtgcg
    46021 ctcggcgact ttcatactgg tgatatccac gcaaaactca cacagacaca tttgcgcaat
    46081 gcgcgcgctg acaagcgatc gcactactgg atccagcggt gagcgtttgc gctccagcca
    46141 gccgacaaac atcgacacca gataaaagat aaacgggatc cgcccccacc agcgaatcgg
    46201 gcttagcacc tcgccgtaat ggcgacgatg cagccacgcc tgcggacgta aaatgaaagg
    46261 aattttgcta agcggcggta aacccatcag gcattcctct tctggcgctc aagccatatt
    46321 ttcttcgcca taaaaatcac cacggtcagg gcaaacagaa taaacagtcc ggtgacaaac
    46381 cagaaagtac cgcctgttaa tatgctgccc gcccaggaat aaacaatagt cgctggtaac
    46441 tgaccaagcc cggtggcgat aaaaaacgag cgaaaacgta ttgaagtcaa accggcagca
    46501 tagcttacgg gatcaaaagg cacaaaaggc aataatcggc ataccagaat ggtgtgttta
    46561 ccatagcggg taaaaaagcc gtccatacta ttgagtaccg ttttacccgt taacttttcc
    46621 accacttcgc ggcccatcac tctggcgata aaaaagcaca gcgccgcgcc ggccatcgaa
    46681 ctggtccacg acagcaagcc gccccagagc gcgccaaaca gcgacgcatt ggcaaaggtg
    46741 atcaaaaacg caggtagcgg tgcagcaatg gcctgtaaaa tcatcaacaa gaacgagaca
    46801 actgccgcca gtgcgccgta agactgaata aagcgttcta taccctgttg gtccacggcg
    46861 gcaaacgccg caacgctgcg attaataaac tcatggacgc cgggaagcag cgcccatgcc
    46921 agcagcatag cgaacaacag gatgatgagg gttatacggt agtaccagat ttttcgcgat
    46981 tgcatcttca tcatccttta tcctggcttt attcggtgat tttaaaggta accgttgcca
    47041 gcgtgttatc cgccgggaga accgaggcat tgcctttgaa tttcacttcg ccgcgttttt
    47101 ccaccgcacc ataagtgtat gttgcattgc tgacgatgcc gaccgggcag ctatccagac
    47161 acaccaggca gcccgttttc ttctcttctg ctgccgttaa attaccgcca aagcgcatgt
    47221 ccagtttctt gccattactg tcaacaatca cttcatcgaa ggaatacgct tttgccgccc
    47281 cttgccagtt gaccgaaata tccagtttgc tgcctgcgac gtgagtcgtt tctttattat
    47341 ccatcgtcat gttttcgccc ggtgtaccac ctgcctcttt cagggcttca taaaatgctt
    47401 tcggggtcgc ataacccata aacagcgatt tatgcccgtt tgagccatct ttaaacacaa
    47461 tgccgtggcg ggtgtcgtcg gtcaggaaac gaccattaat ttgcaccagc atggttaccg
    47521 tatggttatt ggcatcgaca cgcagcggtt ggctgttcga aagtccgtcg tattccactt
    47581 ttgcagccgc attctctttt tgatcgcagc cgctaagccc ggcaacagcc agtaagcaga
    47641 gtgcagccag tccttttttc catgacgctg aataatgttg caacatcgtt ctatccttca
    47701 ttttttgggt gagtaagtgg gctgcggcgc gaagcagaca gatccacatg tttgtgtcgg
    47761 gcgtagagtt tggcgagctg gacgagaata aacagcgcca ggcccgccag acagagttgt
    47821 aaaataaagc gcagcgtaat gccttcattg gcgagatcgc ttgccatcac ggtataaata
    47881 acaataccgg gcagggtcgt gagtgccgaa ataagggtat aaggccagaa ggcgatggtg
    47941 gttaatccgt aagcgtaatt ttgaatattg taaggaaaca acgggattaa gcgggtcaga
    48001 ataagaaaat caataccgtt acgcgcaatg cctttttcaa tggcctggaa ggtatggcta
    48061 tggccaacgt atttcagcag taaatcacgt cccagccagc gcgccagcag gaatgagcac
    48121 gaggaggcca gcgtggcggc aattaatgat agtagtgtcc ctaagagcgg gccaaaaact
    48181 attcctccag cgatcaccag tatgcttcct ggtaacagaa agagggtggc aatgatgaat
    48241 aacagaatat agaggctata accgaacaat ccgctctggc gaatgagtgt ctgtaagtgg
    48301 ggtaaatcgg tgagcagatc gaataaaccg aaagcgtgga tagcgtaaat gagcagcgca
    48361 aaaataagac aggcaaaaag aaatttacgc tcagcgttca ttgtggagtt tcctatatca
    48421 aacttgataa tagagaagtg gtcagacaac taaaaacgca gtgacggtat aacaaagggc
    48481 ggcaggcaac aaatcattac tgacagtttg ttgatttttc tcaaggttaa ttctcctgac
    48541 ccagtttgag ccaggagaac agctaaatta gcggcggaag gtcgcccaga cgggggcatg
    48601 atctgacggt ttttccatgc tgcggatttc atagtcgatg ccggtttcaa cacaacattc
    48661 tgccagcggt tggctggcga gaagcaggtc gatgcgcaga ccacggttat cgtcaaaacc
    48721 ttttgagcgg taatcaaacc atgagaaacg atcggctgtt tgcggattcg catggcggaa
    48781 ggtatcgacc aacccccagc tcatcagtct ctccatccat tcgcgttctt ccggaaggaa
    48841 agagcatttt ccggtacgca gccagcgctt acggttctct tcgccaatgc caatatccag
    48901 gtcgccagga ctgatattca tatcacccat aatcagcacc ggattttcac gcttgagttc
    48961 ggtttccagg tagttttgca gattctgata aaactgcgcc tttgccggga attttatcgg
    49021 atggtcgcgg ctttcaccct gcgggaagta accgttgatc acggtaacat tgcccagcgt
    49081 cgaggggatt tcagccataa taattcgccg ttgcgcctct tcgtcatcac ccggaaaacc
    49141 gcgacgcacg gcaatcggcg tctctttggt cagcagcgcc acgccataat ggcctttctg
    49201 cccgtgataa aacacgttgt agccgagctt cgccacctct tcgagtggaa acatatcgtc
    49261 atgaactttt gtctcctgca ggccaatcac atccggttgg tgcttttcga cgatggcttc
    49321 aagctgatga ggtctggcgc gcaggccgtt gatattaaaa gagacaaatt tcatagtcgc
    49381 tgccatttat tcagataaag agtgcgtgga tggtagcaga atttcgcttt gcgtaccgcc
    49441 tgttgttaag tgatttgatc gatagttgtt aatgatgtca acgatggcgc aaaaaatgcc
    49501 cgcttttggt gcgcgctgcg tcagaatggc gcagtaattt ccagtaaatt tcgtcaatga
    49561 tcacaattcc agaattatat tttacttctg cataacattg cgttttttat tctttttatt
    49621 cacccacatc gcaaacgtat tcactttata tgcactttaa atgcatatgg tttggttata
    49681 actccttgat ttccagttag cctccgcgat ttatgcactt ttatcactgg ctggcacgaa
    49741 ccctgcaatc tacatttaca gcgcaaacat tacttattat taacatataa ataacgaatt
    49801 atttactgta gaggtcgcta tgtctcagcc aattacgcgt gaaaactttg atgaatggat
    49861 gatacctgtt tacgctccgg caccgtttat accggtacgt ggcgaaggtt cgcgcttgtg
    49921 ggatcagcag gggaaagagt atatcgactt cgcgggtggc attgcggtga acgcgctggg
    49981 ccatgcgcac ccggaactgc gtgaggcgct gaacgaacag gcgagtaagt tctggcatac
    50041 cggcaacggt tacaccaacg agccggtact gcgactggcg aaaaaattga ccgacgccac
    50101 gtttgccgat cgcgtcttct tttgtaactc cggtgcggaa gccaatgaag cggcgctaaa
    50161 actggcgcgt aaattcgctc acgaccgcta cggcagccat aagagcggta tcgtggcgtt
    50221 taaaaatgcg tttcatggtc gcacgctgtt tactgtcagt gcgggtgggc agccagccta
    50281 ttcacaggat tttgcgccac tgccgccgga tattcttcat gccgcatata acgatattaa
    50341 ctctgccagc gcgctgattg acgactctac ctgtgcggtg attgtcgaac ccatccaggg
    50401 ggaaggcggt gtggtgccag ccagcaacgc gtttttacat ggtctgcgtg aattgtgtga
    50461 ccgccacaat gcattgctga tttttgatga agtacaaacc ggcgtcggac gcaccgggga
    50521 actgtatgcc tatatgcact acggcgtgac gcccgatctg ttaactaccg ccaaagcgct
    50581 gggcggcggt ttcccggttg gtgcgttgtt ggcaaccgaa gagtgcgcca gtgtgatgac
    50641 cgttggcact catggcacca cctatggcgg caatccgctg gcctcggcgg tggcaggcaa
    50701 agtgctggat ctcatcaaca cgccagagat gcttaatggc gttaaacagc gtcacgactg
    50761 gtttgttgag cgtcttaata ccattaatca ccgctacggt ttgttcagtg aagttcgcgg
    50821 cttaggtttg ttgattggtt gcgtgttgaa tgccgattac gccgggcaag cgaaacagat
    50881 ctctcaggaa gcggcgaaag caggcgtgat ggtactgatt gcgggtggca acgtggtgcg
    50941 ttttgcgcct gcgctcaatg ttagcgaaga agaggtgacg accggactgg atcgctttgc
    51001 agctgcttgc gaacactttg ttagccgagg ttcttcatga tggtcatccg tcccgttgag
    51061 cgttcagata tctcggcgct gatgcagctt gccagcaaaa ctggcggcgg cctgacgtcg
    51121 cttcccgcca atgaagccac gctttcggcg cgtatcgaaa gggcaatcaa aacctggcaa
    51181 ggcgaactgc ccaaaagtga gcagggctat gtgttcgtgc tggaagatag cgagacaggc
    51241 acagtggcgg ggatttgcgc cattgaggtg gcagttgggc tgaacgatcc ctggtacaac
    51301 tatcgcgtcg gcacgctggt tcacgcttcg aaagagctga atgtctataa cgcgttgccg
    51361 acgctgtttc tcagtaacga tcacaccggc agcagcgagc tgtgcacgct gtttctcgac
    51421 ccggagtggc gcaaagaggg caacggctat ttgctgtcga aatcgcgctt tatgtttatg
    51481 gcggcttttc gcgacaagtt taatgacaaa gtggttgccg aaatgcgcgg ggtgattgac
    51541 gaacatggct attcaccgtt ctggcaaagc ctcggtaagc gcttcttttc gatggatttt
    51601 agccgcgccg attttctctg cggcaccggg caaaaggcat ttattgcaga actgatgccg
    51661 aaacatccga tctataccca ctttttatct caggaagccc aggacgtcat cggtcaggta
    51721 catccgcaaa ccgcgcctgc ccgtgcggta ctggagaaag aaggttttcg ctaccgtaat
    51781 tatatcgaca tcttcgacgg tgggccgacg cttgagtgtg acatcgaccg cgtgcgcgcc
    51841 atccgtaaaa gtcggctggt ggaagtggca gaagggcagc ctgcgcaggg cgattttccg
    51901 gcctgcttgg tcgccaatga aaattatcac catttccgcg tggtgctggt gcgtaccgat
    51961 ccggcaaccg agcgtttgat tttaaccgcc gcacaactgg atgccctcaa atgccacgca
    52021 ggggatcgcg ttcgtctggt gcgcctgtgc gcagaggaga aaacagcatg actttatgga
    52081 ttaacggtga ttgggtaacg ggccagggcg cattgcgtgt gaagcgtaat ccggtatcgg
    52141 gcgaggtgtt atggcaaggc aatgatgccg atgccgctca ggtcgggcag gcttgtcggg
    52201 cagcccgtgc ggcgtttccg cgctgggcgc gtctctcctt tggcgatcgt caggtaagag
    52261 ttgaacgctt tgccggattg ctggaaagca ataaagccga attaaccgcg attattgcca
    52321 gagaaacggg taagccgcgc tgggaagcgg caaccgaagt gacggcgatg atcaataaaa
    52381 tcgcgatatc aattaaggcg tatcatgttc gtaccggcga gcagcgtagc gaaatgccgg
    52441 acggcgcggc gagcctgcga catcgcccgc acggcgtgct ggcggtgttt gggccgtata
    52501 atttccctgg tcatttgccg aacgggcata tcgttccggc attgctggcg ggtaacacca
    52561 ttatctttaa acccagcgaa ctgacaccct ggagcggcga agcggtaatg cgtttatggc
    52621 agcaggctgg cttgccgccg ggcgtgctga acctggtgca gggcgggcgt gaaacgggtc
    52681 aggcgctgag tgcgctggag gatctcgacg gcttgctgtt taccggtagc gccaatactg
    52741 gctaccagtt gcatcgtcag ctttccggtc agccggagaa aattctcgcc ctcgaaatgg
    52801 gcggcaataa cccgctgatt atcgatgagg tggcggatat cgatgccgct gtccatctga
    52861 ccattcagtc ggcatttgtc acagccgggc aacgctgcac ctgcgcccgc cgtttattgc
    52921 tgaaaagcgg ggcgcagggc gatgcgtttc ttgctcgtct ggttgccgtc agccagcgat
    52981 taacgccggg caaatgggat gacgaaccgc agccgtttat tggcgggctg atttctgaac
    53041 aggccgcaca gcaggtggtt actgcctggc agcaactgga agcgatgggc agacgaaccc
    53101 tgcttgcgcc gcgcttatta caagcaggga catcgttgct gacgccgggg atcattgaaa
    53161 tgacaggcgt tgctggcgta ccagatgaag aggtgttcgg accgttattg cgcgtctggc
    53221 gttatgatac tttcgatgaa gcgattcgaa tggcgaataa cactcgcttc ggactctctt
    53281 gcggactggt ttcccccgcg cgggaaaaat tcgatcaact gttgctggag gcgcgggcgg
    53341 ggattgttaa ctggaacaaa ccgcttaccg gcgctgccag taccgcgcca ttcggcggca
    53401 ttggcgcttc cggtaaccat cgccccagcg cctggtatgc cgcagattac tgcgcctggc
    53461 cgatggcgag tctggagtcg gactcgttaa cattgcccgc cacgcttaac cctgggctgg
    53521 atttttccga tgaggtggtg cgatgaacgc ctgggaaatc aatttcgacg ggctggtagg
    53581 gctgacgcat cattacgcgg gcctgtcgtt tggtaatgaa gcctccacca gtcaccgttt
    53641 tcaggtctct aacccgcgac tggcggcgaa gcagggctta ctgaaaatga aagcccttgc
    53701 cgacgcggga ttcccccagg cggtgatccc gccgcacgag cgtccgttta ttccggtgct
    53761 gcgtcagttg ggtttcagcg gcagcgatga gcaggtactg gaaaaggttg cccgccaggc
    53821 accgcactgg ctttccagcg tcagttccgc ttcgccaatg tgggtagcta atgcggccac
    53881 ggtagcgcca tccaccgata cgctggatgg caaagtgcat ctcaccgttg ctaacctgaa
    53941 caataaattt caccgttcgc tggaagcgcc cgtctctgaa tcgctgttaa aagcgatttt
    54001 taacgacgaa gagaaattta gtgtccattc ggcgttgccg caggtagcct tgctcggtga
    54061 tgagggggcg gcaaaccaca atcgtctcgg cggtcattac ggtgaaccgg gtatgcaact
    54121 ttttgtctac gggcgagaag agggcaatga tacccggcct tcccgttatc cggcgcgaca
    54181 gactcgcgaa gccagcgagg cggtggcaag gttgaatcag gtgaatcccc aacaggtgat
    54241 tttcgcccag caaaacccgg acgttatcga ccagggcgtt tttcataatg acgtgattgc
    54301 cgtgagtaac cgccaggtgc tgttttgtca tcaacaggcg ttcgctcgac aggtgcagtt
    54361 actggcaaac ctgcgtgcgc gggtgaacgg ttttatggcg atagaagttc cggcgactca
    54421 ggtttccgtg tcggatgcgg tgtctacata tctgtttaac agccaactgt tgagccgcga
    54481 tgatggttcc atgatgttgg tgctgcctca ggagtgtcgg gaacacgccg gagtatgggg
    54541 ttatctcaat gaactccttg ccgctgacaa cccgattagc gaactaaaag tctttgattt
    54601 acgtgaaagc atggcgaatg gtggcggccc ggcgtgcctg cggttgcggg tggtattgac
    54661 agaagaagaa cgccgggcgg tgaatccggc ggtgatgatg aacgatacgc tgtttaatgc
    54721 gctcaatgac tgggtggatc gttactaccg cgatcgtctt actgctgccg atctggccga
    54781 cccgcaattg ctgcgcgaag ggcgggaagc actggatgta ttgagccaat tactgaatct
    54841 cggttcggtt tatccgttcc agcgcgaggg agggggcaat ggataatttt cttgctctga
    54901 ccttaacggg taaaaaaccg gttatcaccg agcgagaaat caacggcgtt cgctggcgct
    54961 ggctgggcga tggtgtgctg gaactgacgc cattaacgcc accgcaaggc gcactggtga
    55021 tttcagcggg aatacacggt aatgagacgg cacctgtgga gatgctggac gcgttgcttg
    55081 gcgcgatatc tcacggcgag atcccgttac gttggcggtt gctggtgatc ctcgggaatc
    55141 ctcctgcgct gaagcaaggg aaacgttatt gccatagcga tatgaaccga atgtttggcg
    55201 gtcgttggca gctatttgct gaaagcgggg aaacctgtcg cgcgcgcgaa ctggaacagt
    55261 gcctggaaga tttttatgac cagggcaaag aatctgtgcg ctggcacctt gatctccata
    55321 ccgcaattcg tggctccttg catccgcagt tcggtgtatt accgcaacgc gacattccct
    55381 gggacgagaa atttctgacg tggctggggg cggcggggct ggaggcactg gtattccatc
    55441 agacgcctgg tggtacgttt actcatttca gcgccagaca ttttggcgcg ctggcctgta
    55501 cgctggaact cggcaaagcg ttgccctttg gacaaaacga tcttcgccag tttgcagtca
    55561 ctgccagcgc cattgctgcg ctgctatctg gtgagagtgt tggtatcgtg agaacgccgc
    55621 cgctccgtta tcgggtggtt tcgcaaatta ctcgccactc gccgtccttc gaaatgcata
    55681 tggcaagtga cacgctgaat tttatgccgt ttgagaaagg aacattgctg gcgcaggacg
    55741 gggaggaacg ttttaccgta acccatgatg tagagtatgt gttattccct aatcctttgg
    55801 tagcgttggg attacgcgcg ggattaatgc tcgaaaaaat aagcta
//
LOCUS       DAOTRK010000011        54913 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.011, whole genome shotgun sequence.
ACCESSION   DAOTRK010000011 DAOTRK010000000
VERSION     DAOTRK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.011"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..171)
                     /locus_tag="QIE41_000759"
     CDS             complement(<1..171)
                     /locus_tag="QIE41_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HDS3529680.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQ"
     gene            506..853
                     /gene="grxD"
                     /locus_tag="QIE41_000760"
     CDS             506..853
                     /gene="grxD"
                     /locus_tag="QIE41_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDS3529681.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(904..5519)
                     /locus_tag="QIE41_000761"
                     /pseudo
     CDS             complement(904..5519)
                     /locus_tag="QIE41_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5612..6259)
                     /gene="rnt"
                     /locus_tag="QIE41_000762"
     CDS             complement(5612..6259)
                     /gene="rnt"
                     /locus_tag="QIE41_000762"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDS3529682.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(6362..6769)
                     /gene="gloA"
                     /locus_tag="QIE41_000763"
     CDS             complement(6362..6769)
                     /gene="gloA"
                     /locus_tag="QIE41_000763"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDS3529683.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6850..7947)
                     /gene="nemA"
                     /locus_tag="QIE41_000764"
     CDS             complement(6850..7947)
                     /gene="nemA"
                     /locus_tag="QIE41_000764"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDS3529684.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7984..8583)
                     /gene="nemR"
                     /locus_tag="QIE41_000765"
     CDS             complement(7984..8583)
                     /gene="nemR"
                     /locus_tag="QIE41_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDS3529685.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIVLLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            8686..8925
                     /locus_tag="QIE41_000766"
     CDS             8686..8925
                     /locus_tag="QIE41_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDS3529686.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8974..9870
                     /locus_tag="QIE41_000767"
     CDS             8974..9870
                     /locus_tag="QIE41_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDS3529687.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9951..10472
                     /gene="sodC"
                     /locus_tag="QIE41_000768"
     CDS             9951..10472
                     /gene="sodC"
                     /locus_tag="QIE41_000768"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDS3529688.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10473..12485)
                     /locus_tag="QIE41_000769"
     CDS             complement(10473..12485)
                     /locus_tag="QIE41_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDS3529689.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSGARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(12485..13342)
                     /locus_tag="QIE41_000770"
     CDS             complement(12485..13342)
                     /locus_tag="QIE41_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDS3529690.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(13345..13581)
                     /locus_tag="QIE41_000771"
     CDS             complement(13345..13581)
                     /locus_tag="QIE41_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDS3529691.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13782..14216
                     /gene="slyA"
                     /locus_tag="QIE41_000772"
     CDS             13782..14216
                     /gene="slyA"
                     /locus_tag="QIE41_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDS3529692.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(14263..14730)
                     /gene="slyB"
                     /locus_tag="QIE41_000773"
     CDS             complement(14263..14730)
                     /gene="slyB"
                     /locus_tag="QIE41_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDS3529693.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            15004..16113
                     /gene="anmK"
                     /locus_tag="QIE41_000774"
     CDS             15004..16113
                     /gene="anmK"
                     /locus_tag="QIE41_000774"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDS3529694.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            16211..16540
                     /gene="mliC"
                     /locus_tag="QIE41_000775"
     CDS             16211..16540
                     /gene="mliC"
                     /locus_tag="QIE41_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDS3529695.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            16599..17255
                     /gene="pdxH"
                     /locus_tag="QIE41_000776"
     CDS             16599..17255
                     /gene="pdxH"
                     /locus_tag="QIE41_000776"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDS3529696.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            17384..18658
                     /gene="tyrS"
                     /locus_tag="QIE41_000777"
     CDS             17384..18658
                     /gene="tyrS"
                     /locus_tag="QIE41_000777"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDS3529697.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18720..19580
                     /gene="pdxY"
                     /locus_tag="QIE41_000778"
     CDS             18720..19580
                     /gene="pdxY"
                     /locus_tag="QIE41_000778"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDS3529698.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARKLIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(19624..20229)
                     /gene="gstA"
                     /locus_tag="QIE41_000779"
     CDS             complement(19624..20229)
                     /gene="gstA"
                     /locus_tag="QIE41_000779"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDS3529699.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNDALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(20335..21837)
                     /gene="dtpA"
                     /locus_tag="QIE41_000780"
     CDS             complement(20335..21837)
                     /gene="dtpA"
                     /locus_tag="QIE41_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDS3529700.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(22448..23083)
                     /gene="nth"
                     /locus_tag="QIE41_000781"
     CDS             complement(22448..23083)
                     /gene="nth"
                     /locus_tag="QIE41_000781"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDS3529701.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIRTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(23083..23778)
                     /gene="rsxE"
                     /locus_tag="QIE41_000782"
     CDS             complement(23083..23778)
                     /gene="rsxE"
                     /locus_tag="QIE41_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDS3529702.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(23782..24402)
                     /gene="rsxG"
                     /locus_tag="QIE41_000783"
     CDS             complement(23782..24402)
                     /gene="rsxG"
                     /locus_tag="QIE41_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDS3529703.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPSQLSQLPACGE"
     gene            complement(24406..25464)
                     /gene="rsxD"
                     /locus_tag="QIE41_000784"
     CDS             complement(24406..25464)
                     /gene="rsxD"
                     /locus_tag="QIE41_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDS3529704.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTPSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(25465..27591)
                     /gene="rsxC"
                     /locus_tag="QIE41_000785"
     CDS             complement(25465..27591)
                     /gene="rsxC"
                     /locus_tag="QIE41_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDS3529705.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGSEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWDDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIHQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAVREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAKPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEKVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQKANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(27584..28162)
                     /gene="rsxB"
                     /locus_tag="QIE41_000786"
     CDS             complement(27584..28162)
                     /gene="rsxB"
                     /locus_tag="QIE41_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDS3529706.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPTRMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(28162..28743)
                     /gene="rsxA"
                     /locus_tag="QIE41_000787"
     CDS             complement(28162..28743)
                     /gene="rsxA"
                     /locus_tag="QIE41_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDS3529707.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(28820..29260)
                     /locus_tag="QIE41_000788"
     CDS             complement(28820..29260)
                     /locus_tag="QIE41_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDS3529708.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(29346..29561)
                     /gene="ydgT"
                     /locus_tag="QIE41_000789"
     CDS             complement(29346..29561)
                     /gene="ydgT"
                     /locus_tag="QIE41_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDS3529709.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(29834..29959)
                     /gene="blr"
                     /locus_tag="QIE41_000790"
     CDS             complement(29834..29959)
                     /gene="blr"
                     /locus_tag="QIE41_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDS3529710.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            30202..31242
                     /locus_tag="QIE41_000791"
     CDS             30202..31242
                     /locus_tag="QIE41_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDS3529711.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGQRLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(31278..32279)
                     /gene="add"
                     /locus_tag="QIE41_000792"
     CDS             complement(31278..32279)
                     /gene="add"
                     /locus_tag="QIE41_000792"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDS3529712.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVSFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(32383..33555)
                     /gene="malY"
                     /locus_tag="QIE41_000793"
     CDS             complement(32383..33555)
                     /gene="malY"
                     /locus_tag="QIE41_000793"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDS3529713.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWAEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWIDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(33565..35157)
                     /gene="malX"
                     /locus_tag="QIE41_000794"
     CDS             complement(33565..35157)
                     /gene="malX"
                     /locus_tag="QIE41_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDS3529714.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKTNNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGVPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            35332..36360
                     /gene="malI"
                     /locus_tag="QIE41_000795"
     CDS             35332..36360
                     /gene="malI"
                     /locus_tag="QIE41_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDS3529715.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEEAHSRNLII
                     PARLIAPK"
     gene            36472..37239
                     /gene="hdhA"
                     /locus_tag="QIE41_000796"
     CDS             36472..37239
                     /gene="hdhA"
                     /locus_tag="QIE41_000796"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDS3529716.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            37460..38050
                     /gene="uidR"
                     /locus_tag="QIE41_000797"
     CDS             37460..38050
                     /gene="uidR"
                     /locus_tag="QIE41_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDS3529717.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            38440..40251
                     /gene="uidA"
                     /locus_tag="QIE41_000798"
     CDS             38440..40251
                     /gene="uidA"
                     /locus_tag="QIE41_000798"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDS3529718.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQLWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDFYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            40248..41621
                     /gene="uidB"
                     /locus_tag="QIE41_000799"
     CDS             40248..41621
                     /gene="uidB"
                     /locus_tag="QIE41_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDS3529719.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDITN"
     gene            41660..42925
                     /gene="uidC"
                     /locus_tag="QIE41_000800"
     CDS             41660..42925
                     /gene="uidC"
                     /locus_tag="QIE41_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDS3529720.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(42970..44478)
                     /locus_tag="QIE41_000801"
     CDS             complement(42970..44478)
                     /locus_tag="QIE41_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDS3529721.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYRGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(44579..45754)
                     /gene="manA"
                     /locus_tag="QIE41_000802"
     CDS             complement(44579..45754)
                     /gene="manA"
                     /locus_tag="QIE41_000802"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDS3529722.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPLDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            45953..47599
                     /gene="fumB"
                     /locus_tag="QIE41_000803"
     CDS             45953..47599
                     /gene="fumB"
                     /locus_tag="QIE41_000803"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDS3529723.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            47742..49145
                     /gene="fumC"
                     /locus_tag="QIE41_000804"
     CDS             47742..49145
                     /gene="fumC"
                     /locus_tag="QIE41_000804"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDS3529724.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELAMGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSETEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(49142..50071)
                     /gene="tus"
                     /locus_tag="QIE41_000805"
     CDS             complement(49142..50071)
                     /gene="tus"
                     /locus_tag="QIE41_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDS3529725.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQRINQLKTTFERIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(50148..51449)
                     /gene="rstB"
                     /locus_tag="QIE41_000806"
     CDS             complement(50148..51449)
                     /gene="rstB"
                     /locus_tag="QIE41_000806"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDS3529726.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     VAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKMVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(51453..52172)
                     /gene="rstA"
                     /locus_tag="QIE41_000807"
     CDS             complement(51453..52172)
                     /gene="rstA"
                     /locus_tag="QIE41_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDS3529727.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDHAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            52301..52636
                     /locus_tag="QIE41_000808"
     CDS             52301..52636
                     /locus_tag="QIE41_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDS3529728.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(52633..53355)
                     /gene="folM"
                     /locus_tag="QIE41_000809"
     CDS             complement(52633..53355)
                     /gene="folM"
                     /locus_tag="QIE41_000809"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDS3529729.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(53392..54774)
                     /locus_tag="QIE41_000810"
     CDS             complement(53392..54774)
                     /locus_tag="QIE41_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDS3529730.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
BASE COUNT        13784 a        13816 c        14285 g        13028 t
ORIGIN      
        1 ctgggtcttt ttggtggttg ccgtgctttt ctttttatct gcttttttgg tgatatgagt
       61 agtagcagaa ctctccctgg cttgctttga agcatgggcc ataggcgtta aaggcagggt
      121 ggtaaaaagt aaagcacaga gcgtgatcga aatacggttt atccgcgcca ctgagcgtcc
      181 ctctgttaag gcaggtctat catgcctgcg tctgatttac aaaacttgat gattctaatg
      241 catagaagaa gagaaagtta atagctttta ttgttaaaaa atacgtttcg tcggcgagcg
      301 caaaaaaatg cagcagtgaa agctaaaaat ggtcataaat acgccagttt gccttgcgtc
      361 agggaagaaa cgaatatttg aaatgcaaca aatagagcaa agggataaaa tagcaaaagc
      421 gtgatgaaaa tgttattttc cgatgccgca ctgacccgct acaatgtcag attactgccg
      481 taaagaagtt tgaggaagca agacaatgag caccactatc gaaaaaatcc aacgccagat
      541 tgctgaaaac ccgatcctgc tgtacatgaa aggttcaccg aaactgccga gctgcggttt
      601 ctctgcccag gcagtccagg cgcttgccgc atgtggcgaa cgttttgcct atgttgatat
      661 cctgcagaat ccggacattc gtgcggaact gccgaaatat gctaactggc cgactttccc
      721 gcaactgtgg gttgacggcg agctggtcgg cggttgtgat atcgtgatcg aaatgtatca
      781 gcgtggcgaa ctgcagcagc tgatcaaaga aactgccgct aaatacaagt ctgaagagcc
      841 ggacgcggaa taatccgtcc tgcaatactg acggatgcga acgggcatcc gtcagttctt
      901 tatctatccc caatccagcc cttgtggcga acttgaaaat cccgcctcac gcagcagcgt
      961 aaacatcggc gtttgccgga ccggtagatc attcacttct gttagcgtaa agcgcagccg
     1021 tggttcgcga cgcagtgcgg tggtcagcgc ctggaaaatt tctggggcga gtaattcatc
     1081 tttttcctgc cagaccagca tttttttgcc gccttgcgcc agatagagta acaatttgcc
     1141 gccagaaacg accaccagcg cacccgcccg acgcgttgga accagcgttg ccggatgtgc
     1201 aggccagggc agaagatttc cccacacatt agccggatcg ttggcggaga gcgccactgg
     1261 tgtatagtgg cgcgtttgcg tggcttgtgt cgccagatcg cgcaatcggt caatagtcag
     1321 acgttcagcg aattgcgcac tccccaaacc ttctacaaaa cgacctcgca taattcgccc
     1381 ggagtcttcc atacttcgac aaagcgtttg catcgatgga aacccgccag ggatattttc
     1441 ggctatcacc gcctgacgac tgatgatgcc gtagcggtcg agcatatttt ccgccagcgc
     1501 cagcatcctt tcggtatcgt ttagtggctc cacctgcaat aacgaccagc gtccggccag
     1561 ctttggtgtg ttgtaagata cccgcggcga gacgggttgt gcatagacag gacgcccacg
     1621 acgagcccgg tgactgcggc gagttgaggt gcgcgcgttg gaactgctgc gggtgagggc
     1681 gcgtaacggt gcccaaatat cgctggtgat gacgccttgc cagaccagcg cccataacgc
     1741 ctcttgcagt gccgagggat caaccgattc gccgatttta tcgcgtatcc gttggctgat
     1801 ttgttgtgca aaccaggctc ctccgtcagc cagaaccgtg actatcgctt gctgcagtgc
     1861 cgaacgattc gcctgattcg cttcggcggg agtgaacgat tctgcagcat attcctgtag
     1921 atgcagtgcc accaggccgt catcttcacc cagttttttt tgccccgacc agataaccgc
     1981 accggttgcc agtaattcat cgagcatttc tggcgaatag tcgcgtacgc gggcaggcag
     2041 gatctggctt tcccagagtg aggcgggtaa accgactccg gcaagctgtt cgatcacccg
     2101 catgacgcca tccacgccct cataaacgcc tggcgatgtt gaggcaaaga gtgccgggct
     2161 accatcggtg gcgggtaata cgccctgacg ttccagcagc aatcgcgcat aggtcgtggc
     2221 cgcaacggga cgtgtcgctt ctctggcggc ttgcagcgag cgcaaacgca gacgacgaaa
     2281 tacttcaccg ctcacccaga tgtcttgttg cagattcatc accagcccct gttcgcgcag
     2341 ttgctgaagt tgctcttcga caatggcaat accgagacta aactcatgag ccagttgttc
     2401 agacgtgacc agagcatgag cccggagata gcgcagaaag aggtcgcgaa gcgggtaact
     2461 gactcggtgc aaatcaatct ctggcagtga ctctggtagt cgtacgccga gagcatcgcg
     2521 cagcctggcg gcatcatcca tacaggcaag acgctcctgc ccgctaatca tcgtccggaa
     2581 gattcgtttt actgcaagaa gattttccag atacgacgca atctcttcac tgctgcctgt
     2641 atgccgttgc accaggtctt caacggtcat tggccccagt tcgcgcagca ggtcgaacaa
     2701 tccttcttca cctttcgctc ttctgccagg agccagtcgt tgcaactctt cttccacctg
     2761 gcgaatgacc tgcgggtcga gtaattcccc cggatcgacc tgtccgagta gattgcgcag
     2821 taactcgctg tccagcgaca gtacggatgc ccggcgctct gccagcgggg cgtcgctctg
     2881 gtacataaat tccgcgacat agccgaacaa taaactagcg gcgaaaggcg agggcgtagt
     2941 ggtcgtgaca tcggatattt gaatttcgcc accgttcagg cgacgcatca aacgttccag
     3001 cgcgggaaga tcataaacat cttgcagaca ttcgcgtagg gtttcgagaa tgaccggaaa
     3061 atccggatat ccctgagcga tttccagcaa ctgactggcg cgcagccgtt gttgccagag
     3121 cggggtgcga tggcccggcg tgcggcccgg cattaataat gcccgcgcgg cgcattcgcg
     3181 aaaacgggcg gcgaaaagtg ccgagctgcc taccgcctcg cggacaattt gcagcaactt
     3241 ttctggttca aacaaaaaaa tcgcggcatc ggcaatttac catcggtgtc aggaatgcgc
     3301 gcaacaatgc cgtcatcact ggcaaccaca gacgcgtcag cgccccatag tgcatgtatt
     3361 cgcccggcaa tcgccagcgc ccagggttca tgcacccgtc ttccataggg agagtgcaaa
     3421 ataatacgcc agtcaccaat ttcatcacgg caccgttcga ggaccagatg acgactcccc
     3481 ggaacgatgc ccgtcgcgtt gcgctgctcg tcaatcaacc cctgaatatt ggcgatcgta
     3541 ttttcttctg ccaaccacgg gggaatagtc ccggaaaaga acgcgccatc cgccagtaaa
     3601 tggagaaaat cgccgatcat ctcgcctaat tctgccggac gtccgttacc ttcaccacgc
     3661 cagaagggaa ggcgggcaga acgacccgga gcaggagtca caatcacctg atcgcgggtg
     3721 atttgctgga tccgccatga ggtagcgccg agagtgataa tgtcgttcac ccgcgactca
     3781 tataccatct cctcatccag ttcacccacc cgccgcgaac cggcctgttc ttcaccttcg
     3841 ggtaataaca cgctatacat gccacgatcc ggaatggtgc caccactggt aaccgccaac
     3901 agctgagcgc caggtcgggc ggtcaatatc ccggtctccc tgttccagac cagttttggt
     3961 cgaaaagcag aaaaatcgcc agagggatag cgcccggaaa gcatatccag cgtggcgtca
     4021 aaaacacgtc ttggcagatc tttccacggt gcggcacggc gtacgcggga gtaccattcg
     4081 tctgcctgta atgcatccat cgacgcggcg gcaacggttt gctgcgcaag gacgtcgagt
     4141 ggattatgcg gtggtgtcag gttttccagc ctgcctgcga acatacactc aacaatgact
     4201 gcggaatcga ctaagtcacg cctggttcgg gggaaaaaaa gccctttgga gatacctcct
     4261 acctggtgtc cggcacgtcc aatgcgttgt aacccactgg caacagaaag cggcgttgcc
     4321 acctgaatca ccagatccac cgcgcccata tcaatcccca gttcgagact ggaggttgcg
     4381 accacgcagc gtaattcacc cgatttcagc gcctgttcgg ttattgctcg ttgttcttta
     4441 gagacggagc cgtggtgtga gcgggcaata aaaatgtcgc tactttgtac acggttagag
     4501 gttgcgccgg aggtcgactc gaaatgggcc gcatcaacgg cgatagacgg ggaacgctgt
     4561 aagcgtgcgg cgtaaagctc attcaatcgt gccgtcagtt tttccgccag cccgcgtgaa
     4621 ttagtaaaga caatggtcga gcgatggcgc aacacttcat caaggatacc cgtttcaata
     4681 tatggccaga tggagccttc ccggccggca tggctgtctt cgccggtgcc gctggcgacc
     4741 gatgagacat catccatatt ggcgaccggt acgacaattc gtatctgcgg atggcgcatt
     4801 gcgggcgggt tgactaccgt aaccgggcga tcgccgccaa gaaatgctgc cacatcgctg
     4861 gctgaacgca cagtggcaga aaggccaatt cgctgtgctg aggtgtggag cagcgcatcg
     4921 agccgctcca gacttaacgc cagatgcgca ccacgtttac tgcctgctac cgcgtggact
     4981 tcatcaataa ttaccgtttc gacgccgcgt agcgtttcgc gcgcgcggga ggtcagcatc
     5041 agatagagtg attcgggtgt ggtgatcaga atatccggcg gattacgggt gagtttgctg
     5101 cgttcctgtg caggcgtatc gccagtacgg atccccacgc gaagattgac ttccgtttcg
     5161 ccgcgccgcc gccgttcatc ggcaataccc tgtaacggga tctgcaagtt gcgctgaacg
     5221 tcggtgccaa gggcttttat cggtgaaata tagaggatgc gtgaggtttt acgtttatgc
     5281 gcctcgcggg tatcttcgcc gccttcgcgg aagagctgat cgagggcgta gagaaatgct
     5341 gccagcgttt tcccggagcc agtcggtgca atcaccagcg catgttcgcc tcgcgccgct
     5401 acatgccagg tttgcgactg gacagcggtc ggctgtttaa aggcgcgaag aaaccagtcg
     5461 cgggttgccg gtgaaaacac gtccggcagg ggcgatgaaa ggtttggatt atctgccatt
     5521 aacgctattc cccgtgaata tcatgcatga tgcgtggaat agcctcacta tagatgcctg
     5581 aaccattgca tgtcatcagg catcgactcg attacacctc ttcggcggca ggtagcggcc
     5641 agcctcccag acgtttccag cggttgacga tttcacaaaa cagcacagca gtgcgttcgg
     5701 tgtcgtacag cgcggagtgc gcctgggtgc tgtcgaagtc catgccagcg gtctggcaag
     5761 cctttgacaa tacggtttgt ccgagtgcca gcccggcaag tgcagcagtg tcaaaagtgg
     5821 cgaaagggtg gaacgggtta cgtttcagtg aggcgcgttc tgcggcggcc atcataaagc
     5881 tgtgatcgaa attggcgttg tgcgccacca taatggcgcg gttacagccg ctcgctttaa
     5941 tgcctttacg tacaacttta aaaatttcgt gcagcgcctc gtattcgctg accgcgccgc
     6001 gatcgggatc gttcgggtca atgccgttga aggcgagggc ctctggctgc aaatttgcgc
     6061 cgacaaatgg ttcgacgtgg aaatgtaatg tggtgtccgg catcagccag ccttgttcat
     6121 ccattttcag ggtgatggcg gcaatctcaa gcagcgcatc ggttttggcg ttaaatccgg
     6181 ctgtttcaac atcgatcaca acaggataaa aaccacgaaa acggtcgcac agaccggtaa
     6241 gttgagcgtt atcggacatc tgattctctt aatacggata aattgcagcg cgcattatga
     6301 caaatattac ttgtcgatgc agtaaagatg cgggcgcgat gagttcacgc ccggcaggag
     6361 attagttgcc cagaccgcga ccagcgtctt tctcttcgat taattcaatt ttgtaaccgt
     6421 ccggatcttc cacaaacgcg ataaccgtag tgccgccttt taccggaccc gcttcacggg
     6481 tgacgttacc gccgttctga cggatttttt cgcacgcttc agcggcgtta tcaacgctaa
     6541 gcgcgatgtg accataagca gtgccgagtt cgtatttatc cacgccccag ttgtaggtca
     6601 gttcaatcac cgcttcttcg gtttccgggc cgtagccaac aaacgccagt gagtatttgt
     6661 attccgggtt ttcgctggta cgcagcagtt tcatgcccag cactttggta taaaaatcga
     6721 tggagcgttg cagatcgcca acgcgcagca tggtatgaag aagacgcatc tttttatcct
     6781 caaaatggct aacagattca aaatgttgtt ttagtatagc ggcggctttg cgccgctcgc
     6841 aatgttggat tacagcgtcg ggtaatccgt gtagccttcc gcaccgccgc catagaaact
     6901 ttcagcacgc tgtggattaa gctcagcttt gcgctgcaag cgggcgacca gatccgggtt
     6961 cgcaatccag tcacgaccaa atgccaccgc atcaattaac cctttgccaa tcagcgtttc
     7021 ggctttttct accgtgtatg cacctgcgcc gataatcgga ccgtggaaac gggcgcgtac
     7081 tttttcgcgg aacgcatcag tatacggttc acctcccgcc caatctggtt ctgacatatg
     7141 cagataagca atgccgcgtt tacccagttg ttcaatcaga tacagtgcat cggcttcttc
     7201 attcgggccg ttatccgtgt tctggaaagt accgattggc gaaacgcgaa tgccaatgcg
     7261 atcggccccc cattcttcaa tccctgcatc gaccacttcc agcaccagac gtgcgcgatt
     7321 ttccacgctg ccgccgtact gatcggtacg atggtttgaa gaaggagaaa ggaactgatg
     7381 cagcaaataa ccatgagcag agtggagctc taccagatca aaaccggctt cacgcgcgtt
     7441 agcaatggcc tgacggaaat cattgacgat acccggaatc tcttccagtt caagcgcacg
     7501 cggcatggat gtttcaacac ggatcgcctg accattttca tcgcgcagag aagtacgtgt
     7561 tcccgcgcta agtgcggaag gcgctaccgg tgcctgaccg ccaggttgca ggctggcgtg
     7621 agaaatgcgt ccggtgtgcc acagctgtac ggccatatga ccattttcag catgaacgcc
     7681 agcggtaatt tttttccatg cggcaatttg ttccggacta tggatgccag gtgcaccagc
     7741 ataccctttt gcctgggcag aaatttgcgt ggcttcgcta ataatcaaac cggcactggc
     7801 acgttggcga tagtattccg ccatcaacgg ggtagggatg tcacccggtt caatactgcg
     7861 cagacgcgtc agcggtgcca taaaaatacg gtttgccgcc gtgatcgcgc ccactttcag
     7921 tggggaatac agtttttcag atgacataaa ggctcccggt cgtctggtga taaaaaatgc
     7981 ctgctaaacg gcaggcgtcg caataatggt ttttacatgt gccagcgcgt tttccagtgg
     8041 ctcggaattg cgcgaaattt tggcctgcag attcgcaccc agccatagtg cgtaaagcac
     8101 ttgtgcctgt tgcagcggtt cgccacaaaa ggttaaacaa tggctatcac ggccattttc
     8161 cagcgcctgc gagagcagaa cgatcacgcc gcgagcgcct ttatccatcg cgctacgcat
     8221 atcttctgac agatcgcaca cttcggcaga gagttttact gtcaggcaac cactgatggt
     8281 tccatgttgg caaaactggt tcagtgtttg ctggtaataa gccagtatgc ggtcgcggta
     8341 gttaccttcg ccggattgca gcaactcagt cagtcgctgg tgatatgcgg cgtaatggcg
     8401 ctcaagcatg gcaacgccaa acgcttcttt agagcgaaag tagtgataga aggacccttt
     8461 cggcacttca gcagttttta gtaattcgct tagccccatc ccggtgaatc cacgttgcag
     8521 gcaaagttgc tcgcccgtcg ccaggagatg ttcgcgagta tcatgttcgg tgtgtttgtt
     8581 catgcgttgg agtgtagtag accagtcggt ctagtgcaag caaagttgct actttgggca
     8641 ttaacagctt caataatggt aataatctta tttgaggagg gcgaagtggc ggagcaatta
     8701 gagttttttc ccgtccagag cccgtgccgg ggaatttgcc agtctgacga acgcgggttt
     8761 tgtcgcggct gttttcgtag tcgggatgag cgttttaact ggaataaaat gagcgatggc
     8821 gaaaaacaag aggtgctgcg cctttgtcgg cagcgattaa tgcgcaaatt acgcgcaaat
     8881 aagccagcgt catcagacga accggagcaa ccatcactct tttgagccta atcttgcgta
     8941 tactcctctc atatctgata agaggaagcg attatggttc agcgtattac tattgcgccg
     9001 caaggcccgg agttttcccg ttttgtgatg ggctactggc gattgatgga ctggaatatg
     9061 tccgcccgcc agctggtcag ttttattgaa gagcatctgg atctcggcgt gaccaccgtg
     9121 gaccatgctg atatttatgg tggctatcag tgcgaagcgg cgtttggcga ggcactgaaa
     9181 ctggcacctc acctgcgtga acggatggaa atcgtcagta aatgcggtat cgcgacgacc
     9241 gcgcgtgaag aaaacgtcat tggtcattac atcactgacc gcgatcacat cattaagagc
     9301 gccgaacagt cgctaattaa tctcgcgacc gatcatctgg atctgctgtt aatccaccga
     9361 ccagacccgt taatggatgc cgatgaagtg gcggacgcgt ttaaacatct gcatcagagc
     9421 ggcaaagtgc gtcattttgg cgtatcgaac tttacgcctg cgcaatttgc gcttttgcaa
     9481 tcacgcctgc cgtttaccct tgccactaat caggtggaaa tatccccggt acatcagccg
     9541 ttactgttgg atggcacgct cgaccaactg caacaactgc gtgttcgtcc gatggcgtgg
     9601 tcctgccttg gtggtggtcg tctgtttaat gatgattatt tccagccgct gcgtgatgaa
     9661 ctggctgtgg tggcggagga gttaaacgcg ggctcgattg aacaggtggt ttacgcctgg
     9721 gtattacgtt taccgtcgca gccgctgcca attatcggct caggtaaaat tgagcgcgta
     9781 cgggcagctg tcgaagctga aacactgaaa atgacccgtc aacaatggtt ccgtatccgt
     9841 aaagcggcat tgggttacga cgtaccgtaa gcacttttag gaatagccgc cgttcaaaaa
     9901 tgtgtcactg gtttacactt attcaggaat gcacaatgaa cggaggtcct atgaaacgtt
     9961 ttagtctggc cattctggcg ctggttgttg cgaccggcgc acaagctgcc agtgaaaaag
    10021 tcgagatgaa cctcgtcacg tcgcaagggg ttgggcagtc aattggtagc gtcaccatta
    10081 ctgaaaccga taaaggtctg gagttttcgc ccgatctgaa agcattaccc cccggtgaac
    10141 atggcttcca tattcatgcc aaaggaagct gccagccagc caccaaagat ggcaaagcca
    10201 gcgccgcgga atccgcaggc gggcatcttg atccacaaaa taccggtaaa catgaagggc
    10261 cagaaggtgc cgggcattta ggcgatctgc ctgcactggt cgtcaataat gacggcaaag
    10321 ctaccgatgc cgtcatcgca cctcgtctga aatcactgga tgaagtcaaa gacaaagcac
    10381 tgatggtcca tgttggcggc gataatatgt ccgatcaacc taaaccgctg ggcggtggcg
    10441 gtgaacgcta tgcctgtggt gtaattaagt aattaagagg ccagcgtacc ttgcggtggt
    10501 gcttgctcaa gttgcgaaag cgagcagtac agccgccaga caattgccgc cagctcacgg
    10561 gcggcaggtt gatgatggcg ggcaaggctg tcgcaaatcc gctgtaattc ttcaagtgtg
    10621 gccgccagtg atttttgctg aacgccacgc tcactcatca ctccgcgcaa cagtgaaata
    10681 cagttatccc gtactcgtga taacggatcg gaacgcgatt cccagtcgcg taattgccag
    10741 acaacatgag aacagttcag cagcactaca ccccagcgta ataaccaacg gcgagccagc
    10801 gcatcctggc tgttactcaa ctgactgacg tgatgataag tgagcgattc aaattcgctt
    10861 tcactcagtg ttggatggcg gcttagctga tcgacaaaat cccggcgcag cgcgcgaata
    10921 tgacgacggc ttttacgagc acccgaccct ggacgcaaaa tggcgaacgc taaccacgcc
    10981 agcgcaacgc caacgatttt tgccagatta tcgttaagaa aatcagcaaa atcatacacc
    11041 gggggattag tgacagcgat aaacgaaccc ataaaaacaa tcagttgccc ccacagtgcg
    11101 gcaaatttgg gcatctgcaa tttaagaagc tgcattgtcg ccagcagtgg aaagagaaac
    11161 agtaaaaatt gccacagatc gctaatctgg accatcaggc caaatttgac cacaaagctg
    11221 aatagcgaaa gtaacaccag agtgcgcatc agtagtgaca acgacttaaa cggtgctgcg
    11281 acggcggagt agagtacgca gctaattgct gccagcgtta atgcattggc accggcatcc
    11341 cattgcgaag caatactcca tgcgccaatc atcatcaacg tacaaaatgt acgcagtccg
    11401 ctccacatag cttcggcgtt atcggtatga cgagccaggc cggatgtccg tgggagtctg
    11461 gtatgatcat ccacaccgct ttgcagacga tgtaattcct gactactttg cagataaagg
    11521 cggcaaaaat aacgtagtcg ctgccagaag gcgacgtgcc ggtagtcggc gacgttggtc
    11581 gggcgtagcg gggcgataat acgtgcgacg gtgtaaacat ctgtttgcga actggcgagc
    11641 gccgtcagca gctgttcgag aatttctcgt gtggcacatg gcggtgaggg ccagttgagc
    11701 aacatacgtc gcaggctgga gatgacactg gtcatacgta attgctggtg gagcagcgca
    11761 ttaaggcgtg cgttttgctg gcgaaaacga tagtggctcc agaaagcctg gatacggagc
    11821 aaattcatgg tcagtatctg tccaatcacc ccctcatgtg ctgcacgaat ggcatcggtt
    11881 gtttcaggct gccagagtaa actggcatgt tccagtaatc gggcgtgcat gtttttcaat
    11941 gcggttaaaa gggcagtagc atcggaactg ctcggcagga tcatcatcat catgccgccg
    12001 cacaaaatac cgacaatcac ctcgcaaacg cgcgcctgag cgatatccca cagctggctg
    12061 gcctcagtaa tattaaccat cggaaaggcg atgatggcag ccgtgtagcc cgccagttga
    12121 aatgcatacg cgacattatt cgtgaagtgc gcacaggccc aggtacaaaa gccaagccac
    12181 gccgccatgc tcaaaagaaa aaaccacggc tcattgagcg tatgtccggc aagaagcagt
    12241 gccgcaatgg ctccgagcaa actgccagcg atgcgtccga ggcttttgct gatgacaccg
    12301 ccaacggtgg gaaagctaac cacggcagcc gaggtcatcg cccaataggg ttcatccaga
    12361 tttaaataat aggcaaccgt cagcgccaga cacatggcaa tggtattgcg taacgcataa
    12421 cgccattgcg ccagcgtggc cctgaaccag ggcaaattgc gcaaggacca tgacgatgcg
    12481 ttcattattg acctacggca atgctacagg tggtgcccga caccagcgtg acatcgcctg
    12541 ggactttatc cagcgcaaag cggacgggaa cgcgttgggc aagtcgaacc caggggacgt
    12601 taggtttcac atccggaata aggcttgagt cactttcaac gctctgatca taaatcgccc
    12661 gaccgatact ggaaacgtga ccctgtaacg ttttgttatc actatagagg gtaatttgtg
    12721 ctggtgcacc ttcgcggata tggcgcaact tggtttcttc aaaataacca atgacgtaaa
    12781 acgagtggct gtcgacaagg gcaaacagcg gtttcccggt gtcagcgtaa tcgccgatgc
    12841 gggtagtgag gttagtcacc catccactca ccggggcgcg aatttccgtt tgcgccagtt
    12901 gccattgtgc ctgtttcagc gtcgcctgtg cggcatcaac actggcctgc atcgctttaa
    12961 cattgaggtt agcggtatcg agttcttcgg cagaaataaa attttgtgag agatgacgac
    13021 gacgattcgc ttcgttattg gcttttgcga ggtcagattg cgctttcgcc agctgagcct
    13081 gtgcgttcag ctcggcgatc tgaaagggcg ttttatcgat cgtgagcaga agatcccccg
    13141 cgttaaccag ttggttgtct tttatattca gctcaacaat acgtcccgac acctgagggg
    13201 tgatagaaac ctgttcagcg cgtattttcc cgtcgcgtgt ccacggagat tgcatgtaat
    13261 aattccacag ccaccatccg gcaagaaccg caactaccgc tacaatgatt gttgagaaat
    13321 acttaattgt tttaattgac atagttacca cgcaatcagt attgccaggg caaggcaaac
    13381 gcaaatcgca aacagcgata aatccattaa caagggatgc cagatgtcac cggcgtagat
    13441 ccagccacga agcaggcggt gtacgacaag ccagatgacg aatccaaacg cgaaggcttt
    13501 gaaaaacgga ggaaagtaga cggacgcacc gaacaccaga tcttgtaagg gcaatcctgt
    13561 tgcattgagc ataaacttca caaagtagat tcctttacga ccgaatatat tgcgttagat
    13621 taataaatat tctttaagtg cgaaaaattt acgcgcaatt tctgaaaaat atgtctgacg
    13681 gtaaccaaat gcagcaatac atttgtttta gcaatacaat tgctgcacac tattctaaaa
    13741 gccgcataat atcttagcaa gctaattata aggagatgaa attggaatcg ccactaggtt
    13801 ctgatctggc acggttggtg cgcatatggc gtgctctgat agaccatcgc ctgaaaccgc
    13861 tggagttaac acaaacccat tgggttacgt tacacaatat ccatcagtta cctccagacc
    13921 agtcgcaaat tcaactggca aaagcgattg gcatcgagca gccatcactg gtccgtactc
    13981 tggaccaact ggaagaaaaa gggttaattt cgcgtcaaac ttgtgccagc gatcgtcggg
    14041 ctaaacgtat taaactgacg gaaaaggcag agccgctgat cagcgaaatg gaagctgtta
    14101 ttaacaaaac ccgcgcggaa attttacatg gcatctccgc agaggaactg gaacaactga
    14161 ttaagctcat cgcaaaactt gagcataata tcattgagtt acaggccaaa gggtgaaatg
    14221 aagggggcgt gtggtcagta acctgaccac acgcaaactt atttagcgcg gggaaacggt
    14281 cacctgactg ccattgctgg ccagtacgac acgttggccc ggagagaaac gagtgttgcc
    14341 ttgtttctgt accaccatga tggtattacc atcgtcttta cgaatttcca gctcgacacc
    14401 ctgcgttttg ttcattgcac tctgtacgcc ctgaccggct acgccacctg caacagcacc
    14461 tgctgcagta gccagagaac gcccggttcc gccaccaaca gtattcccca ggaaaccacc
    14521 aagaacagca ccgccaattg cgccgataac gttggaatca tcaccgccct gaatctgtac
    14581 cggacgtacg ttaacgatgg tgccatagct gacattctgt acttgtttcg cttcagaagc
    14641 ggtataaaca tcccctgaca gggtgtcgtt attaacacaa ccgacaagag acagacctac
    14701 cattgaaaca accaatacgc gtttaatcat tgaaaaatct cctgttcacc atgaaacgcc
    14761 acgcaagcat ccctcatggt caaagtatat ggcatatgtg aatccatggt tacatgttct
    14821 accaaatatg agcaaatcat aatgaaagta ggatcaacca attataaaca aaatattcat
    14881 gatgattatt gatttgaaga taatgtttta ctaccaacgg taaaaatcgg tcattccgca
    14941 tagtattcac gaaaagttta tgtttgagtg gtgcgcaaac gtcagattga aaaaggatga
    15001 actatgaaat cgggccgctt tattggcgtt atgtcaggca ccagccttga tggtgttgat
    15061 gttgtgttgg cgacaattga tgaacaccgg gtcgcacagc tggcaagttt gagctggccg
    15121 atcccggtgt ctctgaaaca ggctgtactg gatatttgcc agggccagca gcttacactt
    15181 tcgcagtttg gacagcttga tactcaactc gggcgacttt ttgctgatgc ggtcaatgcc
    15241 ttgcttaagg aacaaaacct gcaggcgaga gatatcgttg cgatcggctg tcacggtcaa
    15301 accgtctggc atgaaccgac gggcgtggca ccacacactt tacagattgg cgataacaat
    15361 caaattgtgg cacgcaccgg aattacggtt gtcggtgatt ttcgccgtcg cgatattgcc
    15421 ttgggaggac aaggcgcacc gctggtacct gcgttccatc atgcgctgct ggctcaccca
    15481 accgagcgac gaatggtgct caatattggc ggcatcgcaa atctgtcact gctcattcct
    15541 gggcagccgg ttgggggcta cgataccggt cctggtaaca tgctgatgga tgcctggatc
    15601 tggcgtcagg caggtaaacc ttacgataaa gatgccgagt gggcacgggc gggtaaagtc
    15661 attctcccac tgctgcaaaa tatgcttagc gacccgtatt tctcgcaacc tgcaccgaaa
    15721 agtaccggac gcgaatactt taactacggc tggctggagc gccatttgcg ccattttccg
    15781 ggtgttgatc cccgagatgt acaggcgaca ctggcagaac tcaccgccgt gaccatttct
    15841 gaacaagttt tgttgagcgg tggctgcgaa cgattgatgg tatgtggtgg aggaagtcgt
    15901 aatccgctac tcatggcgcg tctggcggca ttactgccag gcacagaagt caccaccacc
    15961 gatgccgttg gcattagtgg cgatgacatg gaagcattgg ctttcgcctg gcttgcctgg
    16021 cggacgctgg cgggattacc aggaaatctg ccttccgtca ctggcgcaag ccaggagacg
    16081 gtactcgggg ctattttccc cgctaacccg tgatatcaga gttaactgaa tttttcttac
    16141 cgcgtctgcc gttacacttg gaacgttagg gagggcgtat tgccctccag accaggaaag
    16201 tcttcgggat atgtctatga aaaaactgtt actgatctgt ttgccggtcc ttctcaccgg
    16261 ctgtagcgcg ttcaatcaac tggttgagcg catgcaaacc gatacgctgg aataccagtg
    16321 tgatgaaaaa ccgttgacgg tcaaactgaa taatccgcgc caggaagtca gttttgttta
    16381 cgataatcaa ctactgcatc tcaaacaggg catttctgcg tctggtgcac gttacaccga
    16441 cgggatctat gttttctggt cgaaaggcga ggaagcgact gtctataaac gcgaccgcat
    16501 cgtcttgaat aactgtcagt tacaaaatcc acagcgttga gatttttcca ggggcggcgc
    16561 acaatagcgt cacccactga caatccgtaa agaaaaccat gtctgataac gacgaattgc
    16621 agcaaatcgc gcatctgcgc cgtgaataca ccaaaggcgg attacgccgc cgcgatcttc
    16681 ccgccgatcc attaaccctt tttgaacgct ggctctctca ggcgtgtgaa gccaaactgg
    16741 cggaccctac cgcgatggtg gtcgctaccg tggatgaaca tggtcagcct tatcagcgca
    16801 tcgttttact caaacattac gacgaaaaag gcatggtgtt ttacaccaac ctcggcagcc
    16861 gtaaagcaca tcaaatcgaa aataatccgc gcgttagcct gctgttcccg tggcataccc
    16921 ttgagcgcca ggtgatggtg atcggtaaag cggaacgact ttcgactctc gaagtgatga
    16981 aatattttca tagtcgtccc cgtgatagcc agattggtgc atgggtttcg aagcagtcca
    17041 gtcgcatttc tgcccgcggt atccttgaaa gtaaattcct ggagctgaag cagaagtttc
    17101 aacagggcga agtgccattg ccgagttttt ggggcggttt tcgcgtcagc cttgaacaga
    17161 ttgagttctg gcagggcggt gagcatcgcc tgcatgaccg ctttttgtac cagcgtgaaa
    17221 atgatgcgtg gaagattgat cgtcttgccc cctgaaaaga tgcaaaaatc ttgctttaat
    17281 cgctggtact cctgattctg gcactttatt ctatgtctct ttcgcatctg gcgaaaagtc
    17341 gtgtaccggc aaaggtgcag tcgttatata catggagatt ttgatggcaa gcagtaactt
    17401 gattaaacaa ttgcaagagc gggggctggt agcccaggtg acggacgagg aagcgttagc
    17461 agagcgactg gcgcaaggcc cgatcgcgct ctattgcggc ttcgatccta ccgctgacag
    17521 cttgcatttg gggcatcttg ttccattgtt atgcctgaaa cgcttccagc aggcgggcca
    17581 caagccggtt gcgctggtag gcggcgcgac gggtctgatt ggcgacccga gcttcaaagc
    17641 tgctgagcgt aagctgaaca ccgaagaaac tgttcaggaa tgggtggaca aaatccgcaa
    17701 gcaggttgcc ccgttcctcg atttcgactg tggagaaaac tctgctatcg cggcgaacaa
    17761 ctatgactgg ttcggcaata tgaatgtgct gaccttcctg cgcgatattg gcaaacactt
    17821 ctccgttaac cagatgatca acaaagaagc ggttaagcag cgtctcaacc gtgaagatca
    17881 ggggatttcg ttcactgagt tttcctacaa cctgttgcag ggttatgact tcgcctgtct
    17941 gaacaaacag tacggtgtgg tgctgcaaat tggtggttct gaccagtggg gtaacatcac
    18001 ttctggtatc gacctgaccc gtcgtctgca tcagaatcag gtgtttggcc tgaccgttcc
    18061 gctgatcacc aaagcagacg gcaccaaatt tggtaaaact gaaggcggcg cagtctggtt
    18121 ggatccgaag aaaaccagcc cgtacaaatt ctaccagttc tggatcaaca ctgcggatgc
    18181 cgacgtttac cgcttcctga agttcttcac ctttatgagc attgaagaga tcaacgccct
    18241 ggaagaagaa gataaaaaca gcggtaaagc accgcgcgcc cagtatgtac tggcggagca
    18301 ggtgactcgt ctggttcacg gtgaagaagg tttacaggcg gcaaaacgta ttaccgaatg
    18361 cctgttcagc ggttccttga gtgcgctgag tgaagcggac ttcgaacagc tggcgcagga
    18421 cggcgtaccg atggttgaga tggaaaaagg cgcagacctg atgcaggcgc tggtcgattc
    18481 tgaactgcaa ccttcccgtg gtcaggcgcg taaaactatc gcctccaatg ccatcaccat
    18541 taacggtgaa aaacaatccg atcctgaata cttctttaaa gaagaagatc gtctgtttgg
    18601 tcgttttacc ttactgcgtc gcggcaaaaa gaattactgt ctgatttgct ggaaataatg
    18661 cattaagtgg aaagggggag tgagaaatca ctccccctgg tttttataca gggaacatga
    18721 tgaaaaatat tctcgctatc cagtctcacg ttgtttatgg tcatgcgggt aacagtgcgg
    18781 cagagtttcc gatgcgccgc ctgggcgcga acgtctggcc gctgaacacc gttcaatttt
    18841 ctaatcacac ccaatacggc aaatggactg gctgcgtgat gccgcccagc catttaaccg
    18901 aaattgtgca aggcattgcc gccattgata aattacacac ctgtgatgcc gtattaagtg
    18961 gctatctggg ttcggcggag cagggtgaac atattctcgg tatcgttcgt caggtgaaag
    19021 cagcaaatcc gcaggcgaaa tatttttgcg atccggtaat gggtcatccg gaaaaaggct
    19081 gtatcgttgc accgggtgtc gcagagtttc atgtgcggca cggtttgcct gccagcgata
    19141 tcattgcgcc gaatctggtt gagctggaga tactctgtga gcatccggta aataacgtcg
    19201 aagaagcggt tctggcagcg cgtaaactca ttgcgcaagg gccacaaatt gtgttggtta
    19261 aacacctggc gcgggctggc tacagccgtg accgttttga aatgctgctg gttaccgccg
    19321 atgaagcctg gcatatcagc cgtccgctgg tggattttgg tatgcgccag ccggtaggtg
    19381 ttggtgatgt gacgagcggt ttactgctgg tgaaactgct tcagggggca acgctgcagg
    19441 aggccctgga acatgtgacc gctgcagtat acgaaatcat ggtgaccacc aaagcaatgc
    19501 aggaatatga gctgcaagtg gttgctgctc aggatcgtat tgccaaacca gaacattatt
    19561 tcagcgcaac aaagctctga aatatctcat ttcggcccac aacgacctgt gggccgatgg
    19621 ctattacttt aagccttccg ctgacagcgc gtcttgtact tccggacgtt cagccatacg
    19681 ttgcataaat gccgcaatgt gctctaaccc ttccagattc agtttcaccg cgtatgccca
    19741 gcgcagaacc gtaaacagat aggcatcagc aattgtaaat cgttgcccgc agatccagtg
    19801 ctcatccttc agagcatcgt tcacatattg cagcttcttc tccagctgcg cgcgaactgt
    19861 tggtttgtac tcttccggtg tatcagggcg aaacagaggt gtgaagcctt tatgcagctc
    19921 ggtggcgatg taattcagcc attcgatggt tttatagcgg gaaatactgt ttaccggtgc
    19981 cagcaactgg cggtcgggga cgctgtcggc aagatactgc ataatcgcta cgccttccgt
    20041 cagcaaagta ccatcgtcca gcagcaatgc aggtacctgt cccttagggt taacggcaaa
    20101 gtaatcgtca ccgttttcga gacgtttttt cattaaatcc acactgacga gcgtaaaatc
    20161 ctttccgctc tcacgcaggg tgatatggga agcgagagag caggcgcccg gtttgtagaa
    20221 caatttcatc ggtaactcct tttgtctgtg gtttaaagta tggtagtgcg ttgctgaaca
    20281 aaaaaaaagc cgctaacgaa aagttagcgg cttgtaaaag agtttccctg aattttacgc
    20341 tacagcagct ttcgccgctt tgtctgcagc gtcatcctgc gtcatgcggt gcagtttcgg
    20401 cgcggtcagc agcatcagta cagcaataac ggcagtagcg acaccaatct gcaagaatac
    20461 gcgaccatag acttccagtg acatcagcgg atcggtaacg ttatccggca cagccatcat
    20521 acccgcaaca taaccaccaa tcaggtttgc accggcagtg gtcaggaacc agctacccat
    20581 aatgaagccc atcagacgct gcggaacgag ttgtgcaacc attgccagac ccagaccaga
    20641 gatcataagt tccccgatac tctgcaggcc atagcttgcg accagccagc ttacagacac
    20701 gatacctgcg tcagacgcga attttgcacc cagcggcaga atcaggaacg caccagaaca
    20761 catcaccata ccgattgcaa acttagttgg catcggcagg gtatcgccca tcttgttata
    20821 gatagcggcc agaatcgggc taccaatgat gatccagaac gggttcagtg cctgatactg
    20881 ctcaggttct acggccaggc ccagaatggt gtgttcaacg ttacgaatcg caaagaagtt
    20941 cagtgacgtt ggcatctggc tgtacagcac gaagaagata atggcttcga gcatcaggat
    21001 gaaggcaacg atcattttac gacgcgcagc acctttcatc gcgaaggctt ctttaccgaa
    21061 gataaccaca ataccgaagg caacaacgcc cagcgccata cgcgcaactt cctgattgtg
    21121 cagcagccag gtggcgatag cgatcagtgc cacaacacca ataatggtca gcagcaggtt
    21181 acggtagttg ataggctcga agtctggttt tgaaccgtac tgtttaaccc agcgttggca
    21241 gaaggcgaag ttaacgatag tgatcagcag gcctacaacg ctcaacgcaa acgcaacact
    21301 ccagccgtat ttcgcggcca gccacggggt ggcgatcata gagaagaaag agccgatgtt
    21361 gacggacatg tagtacatgg tgaatgcacc gtccagacgc gggtcgtttt tctcatagca
    21421 tgtagaaagc agagaagacg ggttagcttt aaacaggccg ttaccgaccg caatagccgc
    21481 catacccata taaacgatac cggcgtcgtg accagaccag gcaaccagag cataaccaat
    21541 cgccagcaca atagcgccaa gcataattac gcgtttagta cccagtacct tgtcacctaa
    21601 ccagccgccg atagcgacca gaccataaac cagggcacta aaggaagaga aaagggtgat
    21661 tgaatccgct tcagacatac ccagttgttt aaccaggtaa acagccataa ttccttgtag
    21721 gccgtaataa ccaaaacgtt cccataactc aatcgagaag atgagataga acgccttcgg
    21781 ttgtttgaaa gcgttcaaac tgacgctttc agttggtttt tggtttgcag tggacacttt
    21841 tacctctttt tttacatccc atattaacgg gggtgctttc tcgcgttatg cctaaaaaac
    21901 atacgcgcaa ttgttatagt gggaggggaa acggcaggta atgttcacta tcttgcactt
    21961 tcagacaagt cctttgtaat aatctgttac atataactga ggcggggaat tcaaccattc
    22021 cgggaaatgt tatccagcgt taaatttttc aaaaaacgta acggcagatt ttttcactac
    22081 cctgagaggg ggatgatggc ttaacggcga tatgttctgc tatttagcgt ttttcctagt
    22141 gagatagtcc agtttctgaa aaatagccag tgtaatgttt tgtaggtcaa tatttgttgc
    22201 gtgtgtaaat ttaaatgatt ttaacctagt tcttgcaatg tttttacatt gccacagcat
    22261 cgtgatcttg atcacgaatg aatagaaaat ttcgctatta aaaaaacaat taacctgcat
    22321 ttttggttat ccacaaacgg attacctgat gataacgcta agcaatggca tcattgacgt
    22381 taaaaagatg ttttaaaaga ggataaagaa aggttatcaa tggggtaatc ggtgttaccc
    22441 cttttcttca gatgtcaact ttctctttgt attcacaaag atcttcaata atacaagagc
    22501 cacagcgagg cttgcgggca atacaggtat aacgcccgtg caggatcaac caatggtggc
    22561 agtcgacttt aaactctgcc ggaaccactt tcagtagctt ttcttctacc tgttcgacgt
    22621 ttttccccgg cgcaaattga gtacgattac aaacgcggaa aatgtgcgtg tcgacagcaa
    22681 tagtcggcca gccgaatgca gtgtttaata cgacgttggc tgttttacga cctacaccag
    22741 gcagggcttc aagcgcagca cgatcttccg gaacctcgcc attatgctgt tccagtaaga
    22801 tacggcaggt tctgatgata ttttctgctt tgctgttata aagcccaatg gttttgatat
    22861 aggttttcac cccttcgacg cccagttcaa gcatcgctgc aggtgtatta gccaccgggt
    22921 agagtttcgc cgtcgcctta ttaacactga catcggtcgc ctgagcggaa agcagtacgg
    22981 caatcagcaa ttcaaaaggc gaactgaaat taagctcggt ggtgggatga ggattgttct
    23041 cacgcaggcg agtgaggatc tccaggcgtt ttgctttgtt catcagacat tccctgtttc
    23101 accgtttggc aatgcacgtt cggcagctgc ttcagtacgg cgctttttca ttctttcatc
    23161 aatcaggtat ttccctgcca gcatcagtcc caggccaata aatgcacctg gtggcagcat
    23221 cgccagcagg aaaggggagt cggtgtggaa aatttccacg cgtaatactt ttgcccagct
    23281 acctaacagc gcatctgcac cgtcaaacaa cgtgccattg ccgataattt cgcgtagtga
    23341 acccagcacg aacatggcgc aggttgcgcc cataccaatt gaaaagccgt ccagtgccga
    23401 aagcgccgga ccttttttgg cggcgaaggc ttcagcgcgg cccacaacga tacagttagt
    23461 gacaatcagc ggaataaaaa tccctaatga ttgatacagg ccaaaggcgt aggcgttgat
    23521 cagcatctgt acagcgctga ccaccgaggc gatgatcatc acgtaaatgg gaatgcggat
    23581 ctcggctggc gtccagtgac gcagcgtcga aatggtcaag ttggtcagcg tcagtaccag
    23641 cgtagtcgca agtcctaaac ccagagcgtt agtggcagtg gacgtgaccg ccaatagcgg
    23701 acagaggccg agcagctgga ccagcgcaga gttgtttttc cacaacccct gaacaataac
    23761 gtctttaatt tcgctcacgc tttattctcc acaggcagga agttgagaaa gttgtgacgg
    23821 taacgtctga gcgtacaatc cggcgcgttt taccgcatta accaccgcgc gcggagtaat
    23881 cgtcgcgccg gtgaactggt cgaaatcacc accatctttc ttcaccgccc agtgcgcatc
    23941 atccgcacca ctgatttttt tacccgcaaa atgggtgatc cagtcagaaa ggcgcagttc
    24001 gattttatcg ccaagccctg gcgtttcgtg gtgctctgtc acgcgcgtgc caagtaccgt
    24061 gccgttaaaa tcggctccca ccagcagctg aatcgcaccg gaatagccat ctggcgcggt
    24121 tgtttccaga acggcggcta ccggtttgtc atcctgtttg gcgatgtaaa cccgatgctc
    24181 acctttacct aactctggcg cagttaccag atagcaactc tgtgccagcg cattgttata
    24241 gcgttcggct ggcagcacct gatcaaataa cgctttttgt tgcagactgg cctgttcagc
    24301 aatcgtcgtt ttggtcatct ggttgatggc cgcagttaac cctgtagaac ccgctgcaaa
    24361 tagtgccaac gtaatgccgt gttttcggat agttttcagc atggtttacc ctttgcgatg
    24421 gccgtagacg cgcggacgcg tgtagtaatc gatcagagga accgtgatgt tcgccagcag
    24481 gacggcaaaa gccacgccgt caggatagcc gccgaaactg cggatcaacc agactaataa
    24541 gcccgccagc gcgccgaaaa taagacgacc acgattggtc gtagaagcgg taaccgggtc
    24601 agtcaaaata aagaatgcgc cgagcatagt cgcaccagac agcagatgaa tttggggtga
    24661 tgccagtgtt tctggtgaga acaaccagcc caacgttgcg cataacgcca gcgttactaa
    24721 gaagctgagg ggaatatgcc agcgaatcgc tttctgccat agcaaccaca cgccgccagc
    24781 cagccaggcg agatttaccc attgccaacc agcgcccgcc agaataccgc tgtagatcgg
    24841 atattgcata atctgttcaa ccgaatgacc ggcacggaca gaggttttaa atgtatccaa
    24901 cggtgtcgcc tgactaatgc catcaatacc taagcgtagc gtgttcatat caccaccact
    24961 gggggtatgt ccgctgaaaa taacctgtat ggcgtcgata aaaccaggga tgttgaccgc
    25021 aatttcatgt ggcggtaacc agctggtcat ctgcaccggg aaggagatca gtaagaccac
    25081 ataaccaatc attgccggat taaacgggtt ttgccccaga ccgccataca actgtttagc
    25141 gatgatcacc gcaaacaccg tacccagtac gaccatccac catggcgcga gggggggaat
    25201 acttaccgcc agcaataagc ctgtcagcaa tgctgagtta tctttcaacg ttgcggctac
    25261 cgactgcttg cgtagtttga gtacgagagc ttcggctaac agagcactga ccgacgccaa
    25321 caggatctga acgagagtac cccaaccaaa aaaccacagt tgcgctgcga ttcctggcac
    25381 ggctgcgagc aacaccaaca gcataatgcg cgatgtctgg cgctggttat gggtataagg
    25441 ggagctagct attctgaata ccatttagtc ctcgtttaca accttttgct gggcggcttt
    25501 tttggcctga acgcgggcaa tagccgcggc aactgccgct ttgcgcggat caacctgttc
    25561 ttctggttcc gcattggcct tttgctgttc cagcttgcgt gctttggcgc gggcaatagc
    25621 ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
    25681 ctgttccagc ttgcgtgctt tggcacgggc aatagcggct tcgacggcgg ctttgcgcgg
    25741 gtcaaccttt tcttctggct ccgcattagc ctgttgctgc tccagcttgc gcgctttggc
    25801 gcgggcgata gcggcttcga cggcggcttt gcgcgggtca acctgttctt ctggcttcgc
    25861 attagcctgt tgctgctcca gcttgcgtgc tttggcgcgg gcgatagcgg cttcaactgc
    25921 ggttttacgt ggatcagcaa cggttgctgc gtcgttagtt tgctgcagtt ctgcctgttt
    25981 cgctctggct tgcgctttac gggcttctcg tactgcaata attgcactgt tatccgggcg
    26041 ttcgcccgct ttaatcacaa tcggctgtgt agcctgggcc tgtttctctt tcacccgcgc
    26101 cagagcggca gcaatcgcat ctttatcttt ggctgcaggt tgaacggctg cgctcttatg
    26161 tcgttcaagg cgagccgctt tttcgcgctc cagacgagcc tggcgcgctt cgaaacgcgc
    26221 tttggcttct gcggcgcgct tttcttcctg atgaatagcc gcaatttcag ctttttcctg
    26281 acggaaatat tgcaccaggg gaatattgct cgggcaaacc cacgcgcaag ccccacattc
    26341 aatgcagtcg gcaatgttat gcgtggtagc tttatcgtgt tgctgacctt tgctgaacca
    26401 gtacaactgt tgcggcaaaa gatccgcagg gcaggcgtca gcacaggcgc tacaccggat
    26461 gcagctttgt tcttcctgtg gttcgccaag ctcattggca gagggagcca acagacagtt
    26521 ggtaatcttt acgaccggga catccagcca tggcaaggta aagcccatta gcgggccgcc
    26581 cataatcacc atttgatcgg cagaggggca gaatccggcg tcattcaata aatgacgcac
    26641 tggcgtcccc agccgtgccc agacgttgcc cgggcgagcg attgcttcgc cagtcagggt
    26701 tacgacacgc tcggtaatcg gctcgccatc aataacggca cgtttcactg cataagcagt
    26761 gccgacgttt tgcattaata cgccgatatc ggatgaacgc ccgccatgtg gaacctgctt
    26821 cccggtcaga atgtaggtta attgtttagc accgccagaa ggatatttag ttggaatcac
    26881 ccgcagagaa atatcgtgag agtccgccag caccgcgcgg agcatggaaa tcgcctgcgg
    26941 tttgttatct tcaatgccga taagaatttc gcgtggctgc agaatatgcg caagaatgcg
    27001 aataccctct acgacctgag ccgcgcaatc ctgcatcaaa cggtcatcgg cggtaatgta
    27061 cggctcgcac tcagcagcgt tgataatcaa cgtttcaatc ttatctccgc cgccctgcaa
    27121 tttaacgcct gtcgggaatc ccgcaccacc cagcccggca acaccaaact gatgaatgcg
    27181 ctcgattaac tcttcgcgac tgcgagagcg ataatcgtcc cagccgtcgc gcgggatcca
    27241 gcagtcttcg ccatcagcat caataatcac gcttaattca gctaaagctg aaggatgagc
    27301 cgtagagtgg ggcgcaatag ccgtaacggt acccgaggtg ggcgcgtgaa ctggcagcat
    27361 tttgccgcga ccacgggtaa gcggctggcc gcgcagtact ttatcgccga cgctaacgca
    27421 caactcacct tcagagccaa tatgttgttt cagtggtatg acaaaacgct gcgccagggg
    27481 tacctggcgc aggggcgtac cgttggactg ggttttcatc tccggtggat ggatgccgcc
    27541 gttgaaatcc cagattttat tttttctgaa tgcagagaat aacttaagca tggtgttcca
    27601 cgggaatgat acgcacggga atggtgttca gatcccattt ccaggagtca ggtgtttctg
    27661 caaccggttg caacgagatg cagtgcgtcg ggcacggatc aacacataaa ttgcagcccg
    27721 tacagagatc actcattacc gtatgcatgg cgcgggtagc gccaacgatg gcgtctaccg
    27781 gacacgcctg aatacattta gtgcagccaa tacagttatt ttcatcaata accgccacca
    27841 tccgcgtagg cgttagctct tgcgcttcgc catccagcgg ctgtggctcg acattaagca
    27901 actcagcaat ttttagcatc acagcttcgc cacctggggc gcagcggtta attttttcac
    27961 cgttacagct gatggcttcc gcgtaggggc gacagccggg ataaccgcac tgaccacact
    28021 ggctctgcgg taagatttcg tcaattttct caacgaccgg atcgtcttcc accgcaaaac
    28081 ggcgggaggc ataacccaga atggcgccaa acgccaggcc cagcaggctc acggcggcaa
    28141 cggcaatcca gatagcattc attacaactt caccaaacca ctaaagccca taaaggccag
    28201 agacataaga cctgcggtaa ttaacgcaat ggcattaccg cgaaaaggtg ccgggacatc
    28261 agccacagca aggcgttcgc ggatggcggc gaagagcacc atcaccagcg agaaaccgac
    28321 agcggcggaa aaaccgtaca gcgccgactg caagaaattg tgcccgagat tgatattcag
    28381 caacgccacg ccgagcactg cacagttggt ggtgataagc ggcaaaaaaa tccccagcag
    28441 acggtaaagc accgggctgg ttttgcgcac caccatctcg gtgaactgca caaccacagc
    28501 aatcaccaga ataaacgcca gggtgcgcag gtaaatcaga ttaagcggga tcaaaatcca
    28561 cgtatcgata agccaggcgc aaatagacgc cagcgtcatc acaaacgttg ttgccagccc
    28621 catgcccatc gcggtttcca gctttttgga aactcccata aacggacaga gaccgagaaa
    28681 cttgaccagt acaaagttat tgaccagtac agttccgaca aacagtaaca ggtagtcagt
    28741 catttttcgg cctgaaataa aaaagccgcc tattatccgt taatccagag caggcgacaa
    28801 caggttaact gtaaggttat tacggattca caaacgtcgc tttcacccgc gacgagcgtt
    28861 taaaataggg gacgatcaat gcggttgcca acaaagtaaa cagtaactgg cgaacggcta
    28921 tgccatcttc cacgggtgaa aaagcgaacg cttttactgc cagcaatacg gaaataagca
    28981 gccagataat atagtgttta ggaacacaac gccgacgttt aaagaaagcg atggtcaacc
    29041 acagtgtgta ataccacatg gcgatagcgg taataaaaga aacgccccat aagatttgtg
    29101 tcgtcagtgc ttgttcgccg agcgtttgaa atgtttgcgg agaagataac gcagcggtat
    29161 acagcaacaa cgccagcgtc gtacttaaca gagcgactaa aagccaggcc aatgggccaa
    29221 gcaaccagcc tccaatacgt tgtggcgttg tggtggtcat aggttctcca gaatagcgac
    29281 gcaaacaggc aaatctaatt ttaggggcgt tattataagg cttttataac cgatcgctac
    29341 tttctttatt ggacatagtg ccagacggac ttcggtacct ggccgaggtc aaacaaacgc
    29401 ccgccagaaa ccagctctgc gcgacggtga tcggcagcac gatacatatt gatcagttcc
    29461 tgatcgtccg tcagggtgta attaagatgg tcgtagagtt tttccagact ttcgagtgaa
    29521 ctgattttgc gaaattttaa taagtagtcc tgaacagtca taaaaggtcc ataaaataat
    29581 taataccaaa ggggtaggta ttattagtga tgataaaatt tcaccaaaag tgaacgcttg
    29641 tgttattaca ataataagaa tgcgctgatg gcaaatattc agggatgaaa aactgctctg
    29701 aaaagagtaa cgcgctttac tatctctgac aatcaccaac aacatcgaac aagataataa
    29761 attcctggtt taatatccga caagtgaaaa catgcacccg gacgggcagc atgtcgctct
    29821 acaagcacag agcttacttg tgttgtaccg tagtactctg ttcaggtggc tgatagttgt
    29881 caatgtgact cgccacgcca agaagaatga ctgaaacgac aagaacgatc caacctgtta
    29941 attcaataag acgattcatt acagcccaca tctctgttga ttgatccatt aacttcaggg
    30001 ggtaaatgtt acttagcaat aatagctcca gcaagatttt tactgaggtt tttgcgatat
    30061 ttagcttttg tcgttggaaa attcgctatt tttttgactt aagttaaaca acatccctta
    30121 ttgctggcag gttattaaac tgttgagcga gggtaaagga tagtgtcaaa tagccatcat
    30181 acttcaatga gaggcaatga catgagcgac aacatccgtg ttgggttgat tgggtatggt
    30241 tatgcgagca aaaccttcca tgcgcccctg attgcgggca cgcccgggct ggaactggcg
    30301 gtaatctcca gcagcgatga aacaaaagta aaagccgact ggccaacggt tacggttgtc
    30361 tctgagccga agcatctgtt taacgatccc aacatagacc tgattgtcat ccctacaccc
    30421 aacgataccc atttcccgtt agccaaagcg gcgcttgagg cgggtaaaca tgtggtcgtt
    30481 gataaaccct ttaccgtgac actgtcacaa gcgcgagagc tggaagcgct ggcaaaaagc
    30541 ctggggcgtg tgctgtctgt attccataac cgtcgctggg atagcgattt cctgacgcta
    30601 aaaggtttgc tcgcggaagg cgtactgggt gaagttgctt actttgagtc tcattttgac
    30661 cgcttccgtc cgcaggtgcg cgatcgttgg cgtgaacagg gcggcccagg cagcggtatc
    30721 tggtacgatt tagcaccgca ccttcttgat caggccatta cgctatttgg tttaccggtc
    30781 agcatgacgg tagatttggc acagttacgg cccggagcgc agtcgaccga ttatttccac
    30841 gccatcttgt cctatccgca gcggcgagtc attttacacg gtaccatgct ggcagccgct
    30901 gagtcagcac gttatatcgt gcatggatcc cgaggcagtt atgtaaaata tggcctcgat
    30961 ccacaggaag aacgtctgaa aaatggccag cgtctgccgc aggaagactg gggctacgat
    31021 atgcgtgatg gcgtacttac ccgcgtggaa ggtgaggaac gtgtcgaaga aacgctgttg
    31081 acagtaccag ggaattatcc ggcttactat gcggctattc gtgatgcgtt aaatggcgat
    31141 ggtgaaaatc cggttccggc aagtcaggca atccaggtaa tggagttgat tgagctgggc
    31201 atcgaatccg ccaaacatcg cgcgacgctg tgccttgcgt gaaaagagga tggtgcaatt
    31261 agcaccatcc atttttgtta cttcgcagcg actttttctc gcagtgcgcg tttttcctct
    31321 gcgctgagga aagccatttc cagaccatta atctgcgcct ggcggatttg ctcgcgggat
    31381 aacccagcag ccggcgcggc aacggtatat tcgtgaatga tatccactcc ctgtacgccg
    31441 ggatcgtcag tgttaatgct ggcacgaatg ccatgctcaa ggaacgtttt cagcggatgt
    31501 gccgccagct ctgccacggt gctggtctga atattggagg tcagacagga ttcaatacca
    31561 atttgctgct cggcgagaaa atccatcagc gcccgatctt caatggcttt tacgccatgt
    31621 ccaatacgtt ccgcccccag ttcacgaatc gcctgccaga tgctttccgg cccggcagct
    31681 tcgcctgcat ggacggtaat atgccagccc gcatcacgcg cgcggttgaa gtgagaaagg
    31741 aacagacttc ccgggaaacc aagttcatca ccggctaaat caagtgcggt aatctggtct
    31801 cggtgggcta aaaaggcctc cagctcttgc tgacaggcgg cttcaccgaa ggtccggctc
    31861 ataatgccga taagcttcgc ctgtacacca aaggtgcggc aaccctcacg tacgccatcg
    31921 atcaccgctt cgacaacacc cgctacaggc agctgatgtg ccattgccat gtagcccggt
    31981 gaaaaacgca gctcgacata gtgcaggccg ttacgggctg catcttcaat gttttcaaat
    32041 gacacgcggc gacaggcatc aagagaggcg agaactttaa cgccccagtc aagtttggtc
    32101 agaaagctca caagatcggg ttcgttggca atgacctgaa cgtggggaat cagtgtttct
    32161 agggattgtg caggaagcga gatattatac tggcggccaa gttcaagaat ggtctgggga
    32221 cgaatgttgc catcaaggtg gcgatggata tcagttaatg gcagggtggt atcaatcatg
    32281 gtcgcactct ttttctggat ataaaagtgc gcaccattat aaaaacaaag cagggtaaaa
    32341 atccacgatt gcgcaacgat tttttacgtt gcgcaattgg ggttaacgaa cagcgcggat
    32401 ggcgttaatt aatccagcaa cacctttttc cagtttcgaa cgcgggcacc cggcattgag
    32461 acggacaaaa ccacgacctt cttcaccgta ggtatacccc ggcatgatcg cgaccttctc
    32521 ttgctcgata agcgcttttt gcaacgcgtt gtcgtcaata ttcaacggac gtagatcaat
    32581 ccaggccaga taagtggatt gcgggatctg ccagttgagt tcagggaacg cggcgttcat
    32641 tttatctgcg atatacgtca ggttatcttt cagatagacg cgtaaggcat ccagccacgg
    32701 cgcgccttgc tgataggcgg cgatatgggc agttaacgcc agtactgaag gggaagaaag
    32761 gccatcacgt cctttcagtg ccgataaata ggcatcacgg ctgctgctat tttctataat
    32821 cccgtaagca ccggtcaggg cgggaatatt gaaacttttc gatcccgacg ttagcaatgc
    32881 ccagtctccg cgcgccacat tactccaggg aatatgcggc tgctcggccc aaaccatatc
    32941 catatggatt tcatcggaaa taacccgcac accatgacgc tcgcacaggt cagccatgat
    33001 ctccagttca tcgcacgtcc acactttccc ggtaggattc tgtgggctac acaggagcat
    33061 aattttacat tctggtttcg ccaacacggc ttcaagcttg cccatatcgc aaaaccagcc
    33121 gtcagcccgc ttctctaaag cgacgggcat tactgtgcgc tggttacctt caatggcctt
    33181 gtaaaatgcg tcataggcgg gtgtgtggat caccacgcct tcacctgttt cagaccactg
    33241 acgaatcagt tctgaaacca tatagataac agaagggcca tacaccaccg actgcgtatc
    33301 aattgcggtg taatgctggg tggaaaacca gtgggcaata gccgcgagaa actcatcgtt
    33361 tttccagcgg ctgtagccaa acacgccgtg catcaggcgc tgattcagcg cctcgataat
    33421 gcagggggca gtggcaaaat ccatgtctga aatcgtgaac ggtaacaggt cagcagtgcc
    33481 gaaacggtca gcgacataat cccactgcgt acaccatgtg ccatgacgat ccacgacctt
    33541 tgaaaaatcg aacatatctt atccttatgc ctggacagta tgcatcagac cggccatttc
    33601 atcttttact gactgaactt gtggcccgat aacaacctgc aggttatgtt gattaagttg
    33661 tactacgcca attgcccgat tgtccttcag tgcctgcaca ttaacaagcg acatatcttt
    33721 cacagacaaa cgcagacggg taatgcagtt atcgaggctg acaatattgt cggcaccgcc
    33781 taaggcttcg aggattgccg gaacgttgta acctgattta cccggcacac cggcaacggc
    33841 tttttcgatt gagctggcaa cttcgctatc gcgccccggg gttttcagat tgaagcgggt
    33901 gatagcgaaa cggaagatga cgtagtaaac gacaaaccag attgccgcca ccactggcac
    33961 catgtaccac ttggttgaca gaccatgcaa aataccgaac accacgaagt cgatgatatt
    34021 gccgtcggta ttaccgatgg tgacgccgag cacagacatg acggtgaagc cgaggccggt
    34081 taacagcgcg tggatgacat acagaactgg cgctacgaac aggaacagga attccagcgg
    34141 ttcggtcgtg ccgccaacga cgcaggcgat caggccagaa atcagcagac ctttaatttt
    34201 atggcgattt tccgggcgcg cgcagtgata catagctaac gctgcacctg gcagaccgcc
    34261 gagaaacgca ggcattttac cttgcgaaag gaaacgcgtg gcgctttcag aaaaaccgtg
    34321 agtggtcggg caactcaatt gcgcctggaa gatggtcagt gcgccgctga cggtttgacc
    34381 gcagacttcc tgcgtgccgc ctgcgtcggt aaagcgaatt aatgccacca gaatgtgatg
    34441 cagaccaaac ggcaacagca gacgttcacc ggtaccaaac agcatcggtc cgaaatcacc
    34501 cgcactgttg atcatatggc ccaggccgct aatacccatg gcgaaaatcg gccagactaa
    34561 tggaatcacc agaccgacaa ggcccatcac cagcgaggag atgattggta cgaagcgcgt
    34621 accgccgaag aatgccagcg catccggcag gcggatatta tggaatcgct catgcagcat
    34681 ccagacgata ataccggcga tcaccgcacc gaggatcccg gtatcgatcg actggatccc
    34741 aagaatgctc tgtatgttat tggttttcag aacggcggcg tctgtggttg gcagaatgcc
    34801 tttattggtt aaccagaagt ttaccgcgag gttcattacc gcataaccga cgaagccagc
    34861 gaatgccgct acgcctttgt tttcgcgtgc caggcccagc gggatggcga tacagaacat
    34921 gactggcagg aaactaaaag caaacgagcc aatcttactc atccaggtaa agatagcctg
    34981 caacacgggg ttgcccagga ccgggattag ggtgatgaca tcgtggctac taagggaact
    35041 accaatgccg agcataatgc cgcagaacga caataatgcc acgggtaaca tgaaggtttt
    35101 gcctaactgc tggaagaact cccacagcgt gactttcggt gctgttttcg ccgtcataaa
    35161 acgactcctc gtagagaaca atctggattc agtaaattgc gcgaaaagat aaaacgtttt
    35221 atcaaatttt agtgaggcat aaatcacatt acgcaacgat aatagcgggt ataagataaa
    35281 taaaaggtaa aacgttttat ctgtcacata atcagggagt aggtcatctg catggctacc
    35341 gccaaaaaaa taaccattca tgatgttgcg ctggctgcgg gcgtgtcggt aagtaccgtt
    35401 tcgctggtgc ttagtggcaa agggcgaatc tctaccgcca caggagaacg cgttaacgcc
    35461 gccattgaag agctgggatt tgtgcgcaat cgccaggcgt cggcgctgcg cggcgggcaa
    35521 agcggcgtca ttggtttgat cgtccgtgat ttatctgcgc cgttttacgc cgaattgacg
    35581 gccggattga cggaagctct ggaagcgcag ggacggatgg tgtttttgct tcacggcggt
    35641 aaagacggcg agcagctggc acaacggttt tcactgttac tgaatcaggg ggtcgatggt
    35701 gtggtaattg ccggggctgc aggaagcagc gatgacctgc gacggatggc agaagaaaaa
    35761 gctatcccgg tgattttcgc ttcccgtgcc agttatcttg atgatgttga tacggttcgc
    35821 ccggacaaca tgcaggctgc acagttgttg acggagcatc tcattcgcaa tgggcatcag
    35881 cggatcgcct ggctgggagg gcaaagttcc tcattaaccc gtgcagaacg ggtggggggc
    35941 tattgtgcga ctctactaaa atttggcctg ccgtttcaca gtgattgggt gctggagtgc
    36001 acttccagcc agaagcaagc cgcggaagct atcacggcgc ttttacgtca taacccgacc
    36061 atcagtgccg tggtttgcta taacgaaact attgcgatgg gggcatggtt tggtttgctg
    36121 aaagcagggc ggcaaagcgg ggaaagcgga gtcgatcgtt actttgagca acaggtttcg
    36181 ctggcggcat ttaccgatgc gacaccaacc acacttgatg atatacccgt tacctgggcc
    36241 agcacgccag cgcgggaact tggtaccaca cttgcggatc gcatgatgca aaaaatcact
    36301 catgaagagg cgcattcacg caatcttatt attcccgccc ggctcattgc gccgaaataa
    36361 tcctctcttt atctgctata cctggtagtg tcctttcctc aaggttaatg ataaaaaatg
    36421 tggcacacag gcggtgcctt ttatttttac tgaagttcac aggaggttta tgtgtttaat
    36481 tctgacaacc tgagactcga cggaaaatgt gccatcatca caggtgccgg tgcaggtatt
    36541 ggtaaagaaa ttgccattac attcgcgaca gctggcgcat ctgtggtggt cagtgatatt
    36601 aacgccgatg cagctaatca tgttgtagat gaaattcaac aactgggtgg tcaggcattt
    36661 gcctggcgct gcgatattac ttccgaacag gaactctctg cactggcaga ctttgccgtc
    36721 agtaagctgg gtaaagttga tatcctggtt aacaacgccg gtggcggtgg gcctaaaccg
    36781 tttgatatgc caatggcaga ttttcgccgc gcttatgaac tgaatgtatt ttcttttttc
    36841 catctgtcac aacttgttgc gccagaaatg gaaaaaaatg gcggtggcgt tattctgacc
    36901 attacttcta tggcggcaga aaataaaaat ataaacatga cctcctatgc atcatctaaa
    36961 gctgcggcca gtcatctggt cagaaacatg gcgtttgacc tgggtgaaaa aaatattcgg
    37021 gtgaatggca ttgcgccggg ggcaatatta accgatgccc tgaaatccgt tattacacca
    37081 gaaattgaac aaaaaatgtt acagcacacg ccgatcagac gtctgggcca accgcaagat
    37141 attgctaacg cggcgctgtt cctttgctcg cctgcagcca gctgggtaag cggacaaatt
    37201 ctcaccgtct ccggtggtgg ggtacaggag ctcaattaat acactaacgg accggtaaac
    37261 aaccgtgcgt gttgtttacc gggataaact catcaacgtc tcagctaaat aactggcagc
    37321 caaatcacgg ctattggtta accaatttcc gagtgaaaag tatacgaata gagtgtgcct
    37381 tcgcactatt caacagcaat gataggcgct cacctgacaa cgcggtaaac tagttattca
    37441 cgctaactat aatggtttta tgatggataa catgcagact gaagcacaac cgacacggac
    37501 ccggatcctc aatgctgcca gagagatttt ttcagaaaat ggatttcaca gtgcctcgat
    37561 gaaagccatc tgtaaatctt gcgccattag tcccgggacg ctctatcacc atttcatctc
    37621 caaagaagcc ctgattcagg cgattatctt acaggaccag gagagggcgc tggcccgttt
    37681 ccgggaaccg attgaaggga ttcatttcgt tgactatatg gtcgagtcta ttgtctctct
    37741 cacccatgaa gcctttggac aacgggcgct ggtggtcgaa attatggcgg aagggatgcg
    37801 taacccacag gtcgccgcca tgcttaaaaa taagcatatg acgatcacgg aatttgttgc
    37861 ccagcggatg cgtgatgccc agcaaaaagg cgagataagc ccagacatca acacggcaat
    37921 gacttcacgt ttactgctgg atctgaccta cggtgtactg gccgatatcg aagcggaaga
    37981 tctggcgcgt gaagcgtcgt ttgctcaggg attacgcgcg atgattggcg gtatcttaac
    38041 cgcatcctga tcctctctct ctttccgcgg gctggtgaca actgagcccg cgtttcatat
    38101 cgtaatttct ctgtgcaaaa attatccttc ctgggttcgg agaattcccc ccaaatattc
    38161 actgtagcca tatgtcatga gagtttatcg ttcccaatat gctcgaacga acgttcgatt
    38221 gcttattttg tggcttatgt caacgctgtt ttaaagatta atgcgatcta tatcacgctg
    38281 tgggtagtgc agtttttggt tttttgatcg cggtgtcagt tctttttatt tccatttctc
    38341 ttccatgggt ttctcacaga taactgtgtg caacacagaa ttggttaact aatcagatta
    38401 aaggttgacc aatattttta tcttaatgag gagtccctta tgttacgtcc tgtagaaacc
    38461 ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac
    38521 tgtggaattg atcagctttg gtgggaaagc gcgttacaag aaagccgggc aattgctgtg
    38581 ccgggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgt gggcaacgtc
    38641 tggtatcagc gtgaagtctt tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt
    38701 ttcgatgcgg tcactcatta cggcaaagtg tgggtaaata atcaggaagt gatggagcat
    38761 cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc cgggaaaagt
    38821 gtacgtatca ccgtttgtgt gaacaatgaa ctgaactggc agactatccc gccgggaatg
    38881 gtgattaccg atgaaaacgg caagaaaaag cagtcttact tccatgattt ctttaactat
    38941 gccgggatcc atcgcagcgt aatgctctac accacgccga acacctgggt ggacgatatc
    39001 accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg
    39061 gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt tgcaactgga
    39121 caaggcacca gcgggacttt gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt
    39181 tatctctatg aactatgcgt cacagccaaa agccagacag agtgtgattt ctacccgctg
    39241 cgcgtcggca tccggtcagt agcagtgaag ggcgaacagt tcctgatcaa ccacaaaccg
    39301 ttctacttta ctggctttgg tcgtcatgaa gatgcggatt tgcgcggcaa aggattcgat
    39361 aacgtgttga tggtgcacga tcacgcatta atggactgga ttggggccaa ctcctaccgt
    39421 acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg
    39481 gtgattgatg aaactgcagc tgtaggcttt aacctctctt taggcattgg tttcgaagcg
    39541 ggcaacaagc cgaaagaact gtatagcgaa gaggcagtca acggagaaac ccagcaggcg
    39601 cacttacagg cgattaaaga gctgattgcg cgtgacaaaa accacccaag cgtggtgatg
    39661 tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca
    39721 ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgtgt caatgtaatg
    39781 ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt
    39841 tattacggtt ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa
    39901 gaactgctgg cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg
    39961 gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt
    40021 gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag
    40081 gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag
    40141 aaggggatct tcacccgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc
    40201 tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg aatcaacaac
    40261 tctcctggcg caccatcgtc ggctacagcc tcggtgacgt cgccaataac ttcgccttcg
    40321 caatgggggc gctcttcctg ttgagttact acaccgacgt cgctggcgtc ggtgccgctg
    40381 cggcgggcac catgctgtta ctggtgcggg tattcgatgc cttcgccgac gtctttgccg
    40441 gacgagtggt ggacagtgtg aatacccgct ggggaaaatt ccgcccgttt ttactctttg
    40501 gtactgcgcc gttaatgatc ttcagcgtgc tggtattctg ggtaccgacc gactggagcc
    40561 atagcagcaa agtagtgtat gcatatttga cctacatggg cctcgggctt tgctacagcc
    40621 tggtgaatat tccttatggt tcacttgcta ccgcgatgac ccaacaacca caatcccgcg
    40681 cccgtctggg cgcggctcgc gggattgccg cttcattgac ctttgtctgt ctggcatttc
    40741 tgataggacc gagcattaag aactccagtc cggaagagat ggtgtcggta taccatttct
    40801 ggacgattgt gctggcgatt gccggaatgg tgctttactt catctgcttc aaatcgacgc
    40861 gtgagaatgt ggtacgtatc gtggcgcagc cgtcattgaa gatcagtctg caaaccctga
    40921 aacggaatcg cccgctgttt atgttgtgca tcggtgcgct gtgtgtgctg atttcgacct
    40981 tcgcggtcag cgcctcgtcg ttgttctacg tgcgctatgt gttaaatgat accgggctgt
    41041 tcactgtgct ggtactggta caaaacctgg tcggtactgt ggcatcggca ccgctggtgc
    41101 cggggatggt cgcgaggatc ggtaaaaaga ataccttcct gattggcgct ttgctgggaa
    41161 cctgcggtta tctgctgttc ttttgggttt cggtctggtc actgccggtg gcgttggttg
    41221 cgttggccat cgcttcaatt ggtcagggcg ttaccatgac cgtgatgtgg gcactggaag
    41281 ctgatactgt agaatacggt gaatacctga ccggcgtgcg aatagaaggg ctcacctatt
    41341 cactattctc atttacccgt aaatgcggtc aggcaatcgg aggttcaatt cctgccttta
    41401 ttttgggatt aagcggatat attgccaatc aggtgcaaac gccggaagtt attatgggca
    41461 tccgcacatc aattgcctta gtaccttgcg gatttatgct gctggcattc gtcattatct
    41521 ggttttatcc gctcacggat aaaaaattca aagaaatcgt ggttgaaatt gataatcgta
    41581 aaaaaatgca gcagcaatta atcagcgata tcactaatta atattcaata aaaataatca
    41641 gaacatcaaa ggtgcaacta tgagaaaaat agtggccatg gccgttattt gcctgacggc
    41701 tgcctctggc cttacctctg cttacgcggc acaactggct gacgatgaag cgggactacg
    41761 catcagactg aaaaatgaat tacgcagggc tgataagcct agtgctggcg cgggaagaga
    41821 tatttacgca tgggtacagg gaggattgct cgatttcaat agtggttatt attccaatat
    41881 tattggcgtt gaaggtgggg cgtattatgt ttataaatta ggtgctcgtg ctgatatgag
    41941 tacccggtgg tatcttgatg gtgataaaag ttttggcttt gccctgggag cagtaaaaat
    42001 aaaacccagt gaaaatagcc tacttaaatt aggtcgcttc gggacggatt atagttatgg
    42061 tagcttacct tatcgtattc cgttaatggc tggcagttcg caacgtacat taccgacagt
    42121 ttctgaagga gcattaggtt attgggcttt aacgccaaat attgatctgt ggggaatgtg
    42181 gcgttcacga gtatttttat ggaccgattc aacaaccggt attcgtgatg aaggggtgta
    42241 taacagccag acgggaaaat acgataaaca ccgcgcacgt tcttttttag ccgccagttg
    42301 gcatgatgat accagtcgct attctctggg ggcatcggta cagaaagatg tatccaatca
    42361 gatacaaagt attctcgaga aaagcatacc gcttgacccg aattatacgt tgaaagggga
    42421 gttgctcggc ttttacgcgc aactcgaagg tttaagtcgt aataccagcc agcccaatga
    42481 aacggcgttg gttagtggac aattgacctg gaatgcgccg tggggaagcg tatttggcag
    42541 tggtggttat ttgcgccatg caatgaatgg tgccgtggtg gataccgaca ttggctatcc
    42601 cttttcatta agtcttgatc gtaaccgtga aggaatgcag tcctggcaat tgggtgtcaa
    42661 ctatcgttta acgccgcaat ttacgctgac atttgcaccg attgtgactc gcggctatga
    42721 atccagtaaa cgagatgtgc ggattgaagg cgcgggtatc ttaggtggta tgaactatcg
    42781 ggtcagcgaa gggccgttac aagggatgaa tttctttctt gctgccgata aaggacggga
    42841 aaaacgcgat ggcagtgcgc tgggcgatcg cctgaattac tgggatgtga aaatgagtat
    42901 tcagtatgac tttatgctga agtaacaaat aacgccggag agaaaaatct ccggcgtttt
    42961 ggctgtgaat tactgctgcg gaatagcggg tacagccgga acgtttaatg ccggcattgc
    43021 aaacatccca acaaaatctt ccagtggcat tttctgcccg tttaacgtga tctgaccgtt
    43081 agcatattgc aggctggtgg tgatggtatt gtcctgcaag gtggtcaggc ggaacatctg
    43141 ccccattgct gacgcacctt caacttgctg tttcgccagt ttttttgctt gatcttcctg
    43201 ataaccttcc agcttcgcta cctgagtcat aaactcagtt gccatatcca ccggaatggt
    43261 cagtttcgca tccagagatt taaccgagcg gtctacttcc tgcgccagcg tttgcggcgc
    43321 ttctttagtc gttgccggat ctttcaggaa cagcgacaga ttcagcgcac tttcaccctg
    43381 actgtttttc cagcttagcg gcgcgatagt aatcaccgga tcgcctttca acatcagcgg
    43441 cagggcgcta aagaaggctt ccgtcacttt ctcctgataa agttcgggat tgttggcaat
    43501 ttctggctgt gccagcagcg cctgagtttg cgcgttatat tgctggctaa actgatgcca
    43561 ggcttcacca tcaatctggc cgacttttaa agtcagcttg ccgctgccca gatcctgatt
    43621 ctgtaccttc aggctgttta gcgagtaatc cagttggcta ttgatcgttt taccgtcatt
    43681 gaccagatcc gatttaccgc tgatctccat gccttccagc agtgccagtt ctttgccttc
    43741 cactgaaatg gtcatttttt ccagcgacag tttttgattt cctacgcgct caccaaaact
    43801 tgccagcgtg ctggaaccgt cggttttcag attattaaag gtcaactgta ctttctggtt
    43861 gtattcgtta accgcgtcta tccgaccact ttgcgcctcc ccggaaaggg agatagcttt
    43921 gccgtctctg tctgcattta actggaactc gccgccgcta aaggcgactt tttcatcctt
    43981 ttgctcgtaa ttcagtggct tgagcgaaat atcggaactg gaatcacctc tgtaaccaat
    44041 gcgggagtta atctcaaaag gcgtttcacc ttttgccata tcaaacagtg gcttgctaac
    44101 ttcgttatta accagcgtgg tttgaattga tgccatcgac gggatcaggt tcagtttttt
    44161 tagctgggca agggggaagg gaccatgatc aactgattcg ttgaagatga cgctctgacc
    44221 gcttttaatc cacggatttt ctttcccggc aatgggtttc accaatagtt gcagctgact
    44281 gctgaatacg ccgcgatgat agttttgata actcacttcc aggttggatt caggagcggt
    44341 cagtttgagt tgcgcgttcg cctgcgcgac catgtcttcg agatgggttt caatcttctt
    44401 gcctgtatac catgcgccgc ctgtccagac tacgcctagc gcaacaatga cgcctaccgc
    44461 taccagcgat ttattcataa tgattatcca taaaatgaaa tcaggcggac tggccgcctg
    44521 aaggtgttat aagcctttaa taagcttagc aagagatgtt aattttttca gtaagctctt
    44581 acagcttgtt gtaaacacgc gctaaacggc cgtggccttt gacagtcacc ggtgattcgt
    44641 tggcggcaat aaacgctgat tcacccggtt taagctgtaa ctgctgagaa cctttccaca
    44701 gcgttgcatc gccttcgacg cagaacaaaa tggcggcact ctgctggctg atggtggttt
    44761 ctttatcact aaggtcatgc agcgagaagg caaaatcatc cacaggaatc gggaagtcca
    44821 gttctgcacc ttgtttcacc ggctgggtca acaactggtt agccggtttg gcttcgaact
    44881 tcacattggc aaccagttcc ggaatatcaa tgtatttagg cgtcagaccc gcacgcagca
    44941 cgttatcgga gttcgccatc acttccagcg ccacgccttg caggtaagcg tgcggtgttt
    45001 cagcgaacag gaacatcgct tcgccaggat tcaatttcac cacattcagc aacagcgggg
    45061 agaacagacc gctgtcatcc gggtaaaatt cagaaattaa acgaattgtt tgccacggtt
    45121 caccctgctg gctgtcaagg gccgatttta aaatcgccag cgcgcgggat ttttcttcac
    45181 cctgcatatt caacaggctg gcgaacagtt cgcttaaacg ttcggcatca ggctgttgta
    45241 aaaagtgagc aatcgccgga tgtgcacctg cgaccggctg gagcagggag acaatctcgg
    45301 aaaactcacg aaacgcgttc atcgcaagga aaggcgtcag cgcaaaaacc agctccggct
    45361 tgtggttagg atctttatag ttacgctcgg cggcatccaa cggaatacct gcggcatttt
    45421 ctttggcaaa accgatttca gaattgcgtt tgtttggatg aacctgaatg gagagcggct
    45481 gtgctgcgca taatactttg aacaggaaag gcagttcgcc aaagcgtttg gcaacggcct
    45541 ctccgagcag agtcgattta tcactctcaa tcacatcacg cagtgaaacg atatctccgg
    45601 cggcattctg cactcgtgaa ctgcttttcg gatgtgcgcc catccacagc tcggccatcg
    45661 gctggctgga cggattttcc ataccataaa gttcagtcaa cgccgttttg ctgccccagg
    45721 catagttttg cactgagtta atgagttttt gcatgatcaa tccctgtttt aatgtggaaa
    45781 ttaatcccgc tattaaagca agaatcctac gggaagtaac ctggagccgc aaaaagtcgt
    45841 actagtctca gtttttgtta aaaaagtgtg taggatattg ttactcgctt ttaacagggc
    45901 aacggaacac ccgcccagag cataaccaaa ccaggcagta agtgagagaa caatgtcaaa
    45961 caaacccttt cattatcagg ctccttttcc actcaaaaaa gatgatactg agtattacct
    46021 gctaaccagc gaacacgtta gcgtatctga atttgaaggg caggagattt tgaaagtcgc
    46081 acccgaagcg ttaactttgt tggcgcgtca ggcgtttcat gatgcgtcat ttatgctgcg
    46141 tccggctcac caacaacagg tggccgacat tctgcgtgac ccggaagcca gcgaaaatga
    46201 taaatatgtg gcgctgcaat tcctgcgcaa ctccgacatc gccgcaaaag gcgttctgcc
    46261 aacctgtcag gataccggca ccgcgattat tgttggtaaa aaagggcagc gtgtatggac
    46321 cggtggcggt gatgaagcgg cgctggcacg cggtgtctat aacacttata tcgaagataa
    46381 tctgcgctac tcgcaaaacg cgccgctgga tatgtataaa gaggtgaata ccggcaccaa
    46441 tctaccagcg cagatcgatc tttatgccgt tgatggcgac gagtacaaat tcctctgtat
    46501 cgccaaaggt ggcggttcgg caaacaagac gtatctctat caggaaacca aagcgttact
    46561 gacgccgggg aaactgaaaa attacctggt tgagaagatg cgcacgctgg gtacggcggc
    46621 ctgtcctccg tatcatattg cgttcgttat tggtggaact tctgcagaaa cgaaccttaa
    46681 aacggtgaaa ctggcttccg ctaaatacta tgatgaactg ccaacggaag ggaatgagca
    46741 cggacaggcg ttccgcgatg tggaactgga aaaagaattg ctgatcgaag cgcaaaatct
    46801 tggtctgggt gcgcagtttg gcggtaaata cttcgctcac gacatccgcg tgattcgcct
    46861 gccacgtcac ggcgcatcct gcccggtcgg tatgggcgtc tcctgttctg ctgaccgtaa
    46921 tatcaaagca aagatcaacc gtcaggggat ttggatcgaa aaactggaac ataatccagg
    46981 caaatatatc ccggaagagc tgcgcaaagc gggagaaggc gaagcggtgc gcgttgacct
    47041 taaccgtccg atgaaagaga tcctcgcaca gttgtcgcag tatcccgttt ctacacgctt
    47101 atcgctgaat ggcacgatta tcgttggtcg tgatattgct cacgccaaac tgaaagagcg
    47161 gatggataac ggcgaagggc tgccgcagta tattaaagat catccgattt actacgcggg
    47221 tccggccaaa acgccggaag gttatgcctc cggttctctt ggcccaacga ccgccggacg
    47281 gatggattct tatgtcgatc aactgcaagc gcagggcgga agtatgatca tgctggcgaa
    47341 aggcaaccgc agccagcagg tgacggatgc ctgtaaaaaa cacggcggtt tctacctcgg
    47401 tagtatcggt ggtccggcag ctgtattggc gcagggaagt attaagagcc tggaatgtgt
    47461 tgaatatccg gaactgggaa tggaagccat ctggaaaatt gaagtggaag atttcccggc
    47521 atttatcctt gtggatgata aaggaaatga cttcttccag cagatacaac tcacacagtg
    47581 cacccgctgt gtgaaataaa cagagccgcc cttcggggcg gtttttttac atggcacgaa
    47641 agaccaaaca tttgttatca aatagtaaat aataagtgag ctaaaagttg cttaacgaaa
    47701 gcaaaacaga aagaaaaaat taatcaggtg aggagcaggc catgaataca gtacgcagcg
    47761 aaaaagattc gatgggggcg attgatgtcc cggcagataa gctgtggggc gcacaaactc
    47821 aacgctcgct ggagcatttc cgcatttcga cggagaaaat gcccacctca ctgattcatg
    47881 cgctggcgct aaccaagcgc gcagcggcaa aagttaatga agatttaggc ttgttgtctg
    47941 aagagaaagc gagcgccatt cggcaggcgg cggatgaagt actggcagga cagcatgacg
    48001 acgaattccc attggctatc tggcagaccg gctccggcac gcaaagtaac atgaacatga
    48061 acgaagtgct ggctaaccgg gccagtgaat tactcggcgg cgtgcgtggg atggagcgta
    48121 aagttcaccc taacgacgac gtgaacaaaa gccaaagttc taacgatgtc tttccaacgg
    48181 cgatgcacgt tgcggcgctg ctggcgctgc gcaagcaact cattccgcag cttaaaaccc
    48241 tgacacagac gctgagtgaa aaatcccgtg catttgccga tatcgtaaaa atcggtcgaa
    48301 cccacttgca ggacgccacg ccgctaacac tggggcagga gatttccggc tgggtagcga
    48361 tgctcgagca taatctcaaa catattgaat acagcctgcc tcacgtagcg gaactggcta
    48421 tgggcggtac agctgtgggt actggactaa atacccatcc ggagtatgcg cgtcgcgtgg
    48481 cagatgaact ggcagtcatt acctgcgcac cgtttgttac cgcgccgaac aaatttgaag
    48541 cgctggcgac ctgtgatgcc ctggttcagg cgcacggcgc gttgaaaggg ttggctgcgt
    48601 cactgatgaa aattgccaat gatgtccgct ggctggcctc tggcccgcgc tgcggaattg
    48661 gtgaaatctc aatcccggaa aatgagccgg gcagctcaat catgccgggg aaagtgaacc
    48721 caacccagtg tgaggcatta accatgctct gctgtcaggt gatggggaac gacgtggcaa
    48781 tcaacatggg gggcgcttcc ggtaactttg aactgaacgt cttccgtccg atggtgatcc
    48841 acaatttcct gcaatcggtg cgcttgctgg cagatggcat ggaaagtttt aacaaacact
    48901 gcgcagtggg tattgaaccg aatcgtgagc gaatcaatca attactcaat gaatcgctga
    48961 tgctggtgac tgcgctcaac acccacattg gttatgacaa agccgccgag atcgccaaaa
    49021 aagcgcataa agaagggctg accttaaaag cggcggccct tgcgctgggg tatcttagcg
    49081 aaaccgagtt tgacagctgg gtacggccag aacagatggt cggcagtatg aaagcagggc
    49141 gttaatctgc aacatacagg tgcagccggg gaatgatcaa acgaagcggc tgcgcctgtg
    49201 gtttataacg gtgttgcaca ttgtcggcat cgtaatttaa caactcccca acgtccggca
    49261 cgcccgcgcc attatcccga ttaatcagcg caataagcgg tgtagggcaa gcgtgttgta
    49321 cttgtttttg atctcccttg taccagacgc gggcaatcgg ctgcaccttc accggacgtt
    49381 tgatttttaa cttcgcgtcc tgtggcaggg tagcaatatc ctgatactct ctctccagtt
    49441 ttctttgcca ctcctcgcgc gtccacggtg cgacactgcg cggtgatttc aggctttttt
    49501 ccagctgtgc caggacttca tcacgatgca aattcttaat gatatgttta ttggcccagc
    49561 caaaacgtaa agtggcgggg tcgtgcagaa cagtgagcgt tcggtaagca ttaagggtga
    49621 tcagccccgg caaatgacga tgcacccatt caaaacgcgc cgctgtgggg agttctgact
    49681 caaccgtgac gatacgctcg aacgtagtct tgagttgatt gatgcgctga atatgactga
    49741 ccaacgctgc ttgcgaaagg ttatcgacct ggtaacacaa tacgccaggt agacgaacag
    49801 cggccttgct gctgcgattt tcggactgtt gttgaataaa taaatggcgg aaatgacgta
    49861 acgccagcga ctgcgcgtcg ttgccgagat gttgctttac ctcaatacga ttaagcggat
    49921 tatgctcatc ctcttttttt acctccggca aagagaacac gcgggcaacc agcaatttgt
    49981 gctgttctag ctcagcggca aatgcagcca gttcttgttc catctggcga aaggtagtgt
    50041 tgagtcggtc tacgagatcg taacgcgcca taatattaac cactttagtt acaacatact
    50101 ttattttata gcacagtatc gacagcctga ccaccgtgcg catagtgtca ggcagaggta
    50161 aattgcggga tgttatgcca taacggccaa ctaaacgaga agcgggcacc acccagttcg
    50221 ctggtgtcac aattaaccgt accgcccatt gctagtgcta tagagtggac aatcgccaac
    50281 cccagcccgc agccgccggt tgagcgatcc cggctgggat cgaggcgaac aaaaggttca
    50341 aagatatgtt cgcggttttc aggggcaatc cctgggccat catcctcaac aattaatgtc
    50401 gctctattcc ccgacagtag caggctggtt tcaaccgttg agtgacagta gcgcagggcg
    50461 ttattgagca aattatccag cacgcgctcc attaagcgca tatccaacgc cgcataatgg
    50521 ccttgcatga gcgtttttat ccgtaccatt ttatcgggcg ttactgcctg aatatctgcc
    50581 agatgcgttg acagccataa cggcaggtct ggttcgctaa gatgaagctc attttgtggg
    50641 cgatcgagtc gggcataagt tagcaactct tcaattaaag cttcaagttg actgatatca
    50701 cgattcaatg cctgggattc ggcggcgctc aggttatcac tcatctccag tcgataacgc
    50761 aagcgcacta acggcgttcg cagttcgtga gcgactccgt caataagctg ttttttgctg
    50821 gctatcaagg cgttgatatt gtccgccatc tggttaaatg cgacgccaag gcgttcaaag
    50881 ctcgaaccct catcaaagtg gatacgttca ttgagatgcc catcgccaaa tcgttgcgcc
    50941 gctgcttcca gttttaacat atcctgccag tgcggacgca tccagataaa caccggaaag
    51001 gcgagggaaa tggcaataaa agcgatcagg gcgatatcca gcaatcgcat ctgatggagg
    51061 taataaagat aaggaacagg accaactgcc agcacgtagt ggctgcgcgg gatacgttgc
    51121 aaaaacgtgt actgatcgtc cagggcgaca atttcgccgc cacgcagccg gtgcatggaa
    51181 atatcatcaa gatggtattt actcagtggc tcgactcgca gatcgaaaga gagatttaaa
    51241 tccatctcct tcagcgtctt accccaatcg tgtgggggga tctcacgcaa ttcgctgcgc
    51301 atcagataca gcgaactgtt catcaaatca tccagcgact gtttgcccgc gcgttcggcg
    51361 gtaaatttgt acaccagccc aaccagcaga gacatcacaa ggaagcagac aaacaataac
    51421 aggtaaaact ggataaacag ttttttcatc gcttattccc atgcatgagg cgcaaaaaga
    51481 tagcctttgt tacgcacagt tttaatgcga taaggttctg cggcgttatc gagcagtttt
    51541 tttcttaacc gcgaaatagc cacgtccacg ctacgatcca ggccgtcata actgacgccg
    51601 cgtaaatttt tcagcaatgc atcgcggtcc atgatttgcc cggcatgggt agctaattcc
    51661 cacaataatt cgaaatcagc tgtcgagagc gagatttcag tgttagccag ggtgactacg
    51721 cggttgatag gatcgatggt caacgtgccg aaatgcaggg ctttgtaggg agtcagtgac
    51781 gtttcctgaa gacctttggt cagcgtggct tgctcattct gacgcaaatg caaacgtaaa
    51841 cgcgctaaca aaacagcagg gggcgtcgtt ttgagaatat agtcgcaggc tcccatttcc
    51901 agtgccagga tgtggttcat atcgctatcg agagaggtta gaagaacaat tggtccggac
    51961 cactttgcgc gtaaatcacg acaaatggtc atgccgtcct tgcctggtag catgatgtcg
    52021 agtaacacca aatccggatt ttctcgcaaa atggtttctt cggcatggtc gccgcgcggc
    52081 tctacggtaa cctgcatatc atgttttgcc aggtacgcgg caatcagtga accgacttcc
    52141 gcatcatctt ccacaaatac gatagtgttc atattattca cggtagatat aaaaacgtca
    52201 caatacaccg cgctgttttt acttactatc aatcgtttct aaacaagttt tcatcaggta
    52261 ttctactgat gacttgttat ggaagtgtta aggtaaaaag atggggctgg taatcaaagc
    52321 cgcgctcggt gcgctggtag tgctgttgat tggtgtttta gcaaaaacga aaaattatta
    52381 tatcgccggg ctgatcccac ttttcccgac ctttgcgctc atcgcgcatt atattgttgc
    52441 cagcgaacgc ggcattgaag ccttacgcgc aaccatcatt tttagcatgt ggtcgattat
    52501 tccctatttt gtttacctgg cgtcgctgtg gtatttcacc ggaatgatgc gcttgcccgc
    52561 cgcttttgtt ggttctgttg catgctgggg gataagcgca tgggtgttga ttatatgctg
    52621 gattaagctg cattaacgca gatgacgacc gccatcaaga gggaaactgc gtccggtgac
    52681 aaagcagctg gtaagtaagt aatcgaccag gtcgatcact tctttctcgc caggcgcggt
    52741 tttcatcagt gatttattca gcgcctgttg tcgatattcg gcatcatcat gttcattaaa
    52801 caggatcagc gatggcgcaa tagaattcac tttcacttcc ggtgccagct tgcgggcaaa
    52861 cgaacgggtc atattatcca gtgccgcttt gcttgcagca taggcaatat gtttgtcgct
    52921 accgcgctcc accacataat cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc
    52981 acgcagtaat ctttccaggg catggttgag tagatatggg gtattaacgt ggatctgcat
    53041 catgcaagcc agtacgtcgg tcagtggcgc tcccggtttt tccgccatcc acgcgctggc
    53101 gttatgcaaa atagcacgca ggccatgggt gctttttagt acttcatcgg caaacgccat
    53161 cacaccgtcg ttggtcgaaa aatcagcctg aatacactgc gcacctgcct taatcagtcc
    53221 atcaatggct ggatagtgcg tccgatagct gacaatcacc ggttgctttt gattaatgaa
    53281 atgccaggcg agtgcgaggc cgatgcgacg acctccgcca gtaattaata ttggcaaggg
    53341 ctgggcttta cccatcgtta tctcctttgc tatccaacga tgggatgaac gttatcccac
    53401 cagcatccag gtcgccggaa ctgaggcaat taacagcatg ccaattagca ccatttcctg
    53461 acggttcagt acgttatcat gtgtatgcgt tttacgcgca tacaggaaaa ccagcagccc
    53521 cggcgcatac agaacaacgg ataacagcaa gtgcattgga ccagaggcgt acaataacca
    53581 taagccataa atgcaggcac cgacacctac cgctttatgc agtggacggg ttgcgatttt
    53641 cagcaagaac gcgccgacca ggaaataggg caccagaatc atttctgagg cgatggtcag
    53701 caacgtgttg taatcggaac cggtcagcca gatgagcacc agacaaattt gcacgcagat
    53761 gttggtcaac cacagtgagg cggatggcgc ggcttgtgcg ttctggcgcg caaaaatgcg
    53821 tgggaatgct ttatgagtgg cagccaggaa cggaacttct gccgccatga tggtccagct
    53881 taagtacgcg ccgcaaacgg aaacgatcaa accggcagcg atgatgattt cgccccacgg
    53941 tcccatcatt tccaccatca gaccggccat tgacgggtta cgaatttcag ccagttcagg
    54001 acgtgccacc acgcccagcg aaagcagcgt taccagcaaa taaacgccca gagcggagag
    54061 aaccgccagc agtgtcgctt tgccaacatc acgtttatta cgcgcacgcg cagaaacaac
    54121 cacggcacct tcaacaccaa tgaaaaccca cagggtgatc agcatggtgt ttttcacctg
    54181 ttcccacacg ggtacgccaa gtgcaagtcc ggtgaagtcg agcttgaagg tatccagttt
    54241 gaacatcatc atcgccagca caacaaacag acccagcggc aacaactttg ccagtgtcgc
    54301 caccaggtta atgctggcag cggtttgcac tccgcgcagg atcaaaaagt gaacaatcca
    54361 caataacgcc gatgcaccga cgatcgattg ccaggtatta ccatcaccaa acaggcgcaa
    54421 ttccggcgtg tccgtaaaaa agcttaacgc ggaaaaaacg atcaccagat aggagacgtt
    54481 ggcgatgact gcgcacagcc agtatcccca tgcggaacaa aagccgatta gctcgccaaa
    54541 cccttcgcgg gcataggtaa agataccgcc gtcaagttcg ggacgaatgc gcgtgaggat
    54601 cagcatggca aaggccagca ataaaatgcc agcgccagta ataccccagc cgatgagcag
    54661 tgctgccggg ctggcaactg ccgccatatt ttgcggcaga ctgaaaacac ccgcgcccag
    54721 cattgagctt aataccagcg cggtgagtgc gctcagtccc agtttctttt ccatcgatat
    54781 ccagtgatta aaacgtgaca agaagaataa atattagggc aaaacgcata aaaatggagt
    54841 ttgccactga agcgcgggat tttacgaaga gatgtgtctg catgcaatgg tcgcggggaa
    54901 atttgtgact aaa
//
LOCUS       DAOTRK010000012        54386 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.012, whole genome shotgun sequence.
ACCESSION   DAOTRK010000012 DAOTRK010000000
VERSION     DAOTRK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.012"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            89..1120
                     /locus_tag="QIE41_000811"
                     /pseudo
     CDS             89..1120
                     /locus_tag="QIE41_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1485..1751)
                     /gene="minE"
                     /locus_tag="QIE41_000812"
     CDS             complement(1485..1751)
                     /gene="minE"
                     /locus_tag="QIE41_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDS3529731.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1755..2567)
                     /gene="minD"
                     /locus_tag="QIE41_000813"
     CDS             complement(1755..2567)
                     /gene="minD"
                     /locus_tag="QIE41_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDS3529732.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2591..3286)
                     /gene="minC"
                     /locus_tag="QIE41_000814"
     CDS             complement(2591..3286)
                     /gene="minC"
                     /locus_tag="QIE41_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDS3529733.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3806..4174
                     /locus_tag="QIE41_000815"
     CDS             3806..4174
                     /locus_tag="QIE41_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529734.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4277..4678)
                     /locus_tag="QIE41_000816"
     CDS             complement(4277..4678)
                     /locus_tag="QIE41_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDS3529735.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4749..4907
                     /locus_tag="QIE41_000817"
     CDS             4749..4907
                     /locus_tag="QIE41_000817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529736.1"
                     /translation="MAFLCSVKSRRGIFAGGSLIKGAWTEQTAEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4919..5212
                     /locus_tag="QIE41_000818"
     CDS             4919..5212
                     /locus_tag="QIE41_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDS3529737.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5284..5943
                     /locus_tag="QIE41_000819"
     CDS             5284..5943
                     /locus_tag="QIE41_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDS3529738.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6035..6481
                     /locus_tag="QIE41_000820"
     CDS             6035..6481
                     /locus_tag="QIE41_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDS3529739.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6688..7599)
                     /gene="hlyE"
                     /locus_tag="QIE41_000821"
     CDS             complement(6688..7599)
                     /gene="hlyE"
                     /locus_tag="QIE41_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HDS3529740.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            7971..8390
                     /locus_tag="QIE41_000822"
     CDS             7971..8390
                     /locus_tag="QIE41_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDS3529741.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            8390..9658
                     /gene="umuC"
                     /locus_tag="QIE41_000823"
     CDS             8390..9658
                     /gene="umuC"
                     /locus_tag="QIE41_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDS3529742.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLAGTDIRFIRKHF
                     NVVLERTVRELHGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPPPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(9704..10234)
                     /gene="dsbB"
                     /locus_tag="QIE41_000824"
     CDS             complement(9704..10234)
                     /gene="dsbB"
                     /locus_tag="QIE41_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDS3529743.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLLVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(10380..11921)
                     /gene="nhaB"
                     /locus_tag="QIE41_000825"
     CDS             complement(10380..11921)
                     /gene="nhaB"
                     /locus_tag="QIE41_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDS3529744.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLCWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            12143..12862
                     /gene="fadR"
                     /locus_tag="QIE41_000826"
     CDS             12143..12862
                     /gene="fadR"
                     /locus_tag="QIE41_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDS3529745.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(12912..14444)
                     /locus_tag="QIE41_000827"
     CDS             complement(12912..14444)
                     /locus_tag="QIE41_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDS3529746.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            14774..16072
                     /gene="dadA"
                     /locus_tag="QIE41_000828"
     CDS             14774..16072
                     /gene="dadA"
                     /locus_tag="QIE41_000828"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDS3529747.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16082..17152
                     /gene="dadX"
                     /locus_tag="QIE41_000829"
     CDS             16082..17152
                     /gene="dadX"
                     /locus_tag="QIE41_000829"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDS3529748.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMERIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVMTV"
     gene            complement(17320..18756)
                     /locus_tag="QIE41_000830"
     CDS             complement(17320..18756)
                     /locus_tag="QIE41_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529749.1"
                     /translation="MASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMK
                     YIGPQIFSWGDDHRQIIDLSTAGEVKIHMVDNTIITLERLNPNPQHNEFMMVAEWCQW
                     AMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIEN
                     RAEALRRMKECLITRQSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWL
                     LVLDCTDNVLILLPKVQPSKLMVLKCYENCIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNIHSIPDEMLENLTRLKVFDCSSNDLISAPRLPPKLIIYYCGENKF
                     KTVQVPQPQSLKVFDCNGNPWDKDNLPTLFKAVEGLKKQQGLKDLLGFLHKEGQMKLE
                     KLEKLEDLDDLMDLELLDDPVLLERVKVQEDLELLNQQLGLLILERQQDSQPVNQHSE
                     HEPESASRVKRDLSEVEFESTMKRKRFM"
     gene            19017..19304
                     /locus_tag="QIE41_000831"
     CDS             19017..19304
                     /locus_tag="QIE41_000831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529750.1"
                     /translation="MHPVLQADETPLQLLSSLKRVLCGLRQCGRLRGGICSGDVRLGP
                     LMVDGDTGNAGLVILHLLQSEEAIMLTFLSRRFELISQSLQWFVIHNLTFF"
     gene            complement(19406..21142)
                     /locus_tag="QIE41_000832"
     CDS             complement(19406..21142)
                     /locus_tag="QIE41_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDS3529751.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(21237..22151)
                     /gene="ldcA"
                     /locus_tag="QIE41_000833"
     CDS             complement(21237..22151)
                     /gene="ldcA"
                     /locus_tag="QIE41_000833"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDS3529752.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVDVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEPRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            22251..22862
                     /gene="emtA"
                     /locus_tag="QIE41_000834"
     CDS             22251..22862
                     /gene="emtA"
                     /locus_tag="QIE41_000834"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDS3529753.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(22864..23598)
                     /locus_tag="QIE41_000835"
     CDS             complement(22864..23598)
                     /locus_tag="QIE41_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDS3529754.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            23799..24053
                     /locus_tag="QIE41_000836"
     CDS             23799..24053
                     /locus_tag="QIE41_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDS3529755.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(24103..26073)
                     /locus_tag="QIE41_000837"
     CDS             complement(24103..26073)
                     /locus_tag="QIE41_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS3529756.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(26099..26953)
                     /locus_tag="QIE41_000838"
     CDS             complement(26099..26953)
                     /locus_tag="QIE41_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDS3529757.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(26950..27762)
                     /locus_tag="QIE41_000839"
     CDS             complement(26950..27762)
                     /locus_tag="QIE41_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDS3529758.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTAKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLKVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAMASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVQPEALR"
     gene            complement(27772..28530)
                     /locus_tag="QIE41_000840"
     CDS             complement(27772..28530)
                     /locus_tag="QIE41_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3529759.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAIKH"
     gene            complement(28527..29507)
                     /locus_tag="QIE41_000841"
     CDS             complement(28527..29507)
                     /locus_tag="QIE41_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDS3529760.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDIQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(29507..30529)
                     /locus_tag="QIE41_000842"
     CDS             complement(29507..30529)
                     /locus_tag="QIE41_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDS3529761.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDVPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            complement(30867..32564)
                     /gene="treA"
                     /locus_tag="QIE41_000843"
     CDS             complement(30867..32564)
                     /gene="treA"
                     /locus_tag="QIE41_000843"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDS3529762.1"
                     /translation="MKSPTPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAIANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
     gene            complement(32883..34301)
                     /gene="dhaM"
                     /locus_tag="QIE41_000844"
     CDS             complement(32883..34301)
                     /gene="dhaM"
                     /locus_tag="QIE41_000844"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDS3529763.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(34312..34944)
                     /gene="dhaL"
                     /locus_tag="QIE41_000845"
     CDS             complement(34312..34944)
                     /gene="dhaL"
                     /locus_tag="QIE41_000845"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDS3529764.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(34955..36025)
                     /gene="dhaK"
                     /locus_tag="QIE41_000846"
     CDS             complement(34955..36025)
                     /gene="dhaK"
                     /locus_tag="QIE41_000846"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDS3529765.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            36253..38172
                     /gene="dhaR"
                     /locus_tag="QIE41_000847"
     CDS             36253..38172
                     /gene="dhaR"
                     /locus_tag="QIE41_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDS3529766.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSTLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGHLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(38272..41139)
                     /locus_tag="QIE41_000848"
     CDS             complement(38272..41139)
                     /locus_tag="QIE41_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDS3529767.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKYGAIGLNAIIFSPLL
                     IAADLGSQNGTTITISDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAVANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSSGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSFWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HTSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTIPEPT
                     PNPEPTPAPAQPPIVNPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGNGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(41985..43076)
                     /gene="ychF"
                     /locus_tag="QIE41_000849"
     CDS             complement(41985..43076)
                     /gene="ychF"
                     /locus_tag="QIE41_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDS3529768.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKTAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(43193..43777)
                     /gene="pth"
                     /locus_tag="QIE41_000850"
     CDS             complement(43193..43777)
                     /gene="pth"
                     /locus_tag="QIE41_000850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDS3529769.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            44055..44333
                     /gene="ychH"
                     /locus_tag="QIE41_000851"
     CDS             44055..44333
                     /gene="ychH"
                     /locus_tag="QIE41_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDS3529770.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(44388..46067)
                     /gene="dauA"
                     /locus_tag="QIE41_000852"
     CDS             complement(44388..46067)
                     /gene="dauA"
                     /locus_tag="QIE41_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDS3529771.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(46192..47139)
                     /gene="prs"
                     /locus_tag="QIE41_000853"
     CDS             complement(46192..47139)
                     /gene="prs"
                     /locus_tag="QIE41_000853"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDS3529772.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(47290..48141)
                     /gene="ispE"
                     /locus_tag="QIE41_000854"
     CDS             complement(47290..48141)
                     /gene="ispE"
                     /locus_tag="QIE41_000854"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDS3529773.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(48141..48764)
                     /gene="lolB"
                     /locus_tag="QIE41_000855"
     CDS             complement(48141..48764)
                     /gene="lolB"
                     /locus_tag="QIE41_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDS3529774.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            48978..50234
                     /gene="hemA"
                     /locus_tag="QIE41_000856"
     CDS             48978..50234
                     /gene="hemA"
                     /locus_tag="QIE41_000856"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDS3529775.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            50276..51358
                     /gene="prfA"
                     /locus_tag="QIE41_000857"
     CDS             50276..51358
                     /gene="prfA"
                     /locus_tag="QIE41_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDS3529776.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDVVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            51358..52191
                     /gene="prmC"
                     /locus_tag="QIE41_000858"
     CDS             51358..52191
                     /gene="prmC"
                     /locus_tag="QIE41_000858"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDS3529777.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            52188..52580
                     /locus_tag="QIE41_000859"
     CDS             52188..52580
                     /locus_tag="QIE41_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDS3529778.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIAFDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            52584..53393
                     /locus_tag="QIE41_000860"
     CDS             52584..53393
                     /locus_tag="QIE41_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDS3529779.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            53429..54283
                     /gene="kdsA"
                     /locus_tag="QIE41_000861"
     CDS             53429..54283
                     /gene="kdsA"
                     /locus_tag="QIE41_000861"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDS3529780.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT        13507 a        13411 c        13896 g        13572 t
ORIGIN      
        1 caacagcaac tggaacctga ctaatgatgt caacccaaat ccgaacccaa acccaaatcc
       61 aaacccaaat ccgaatccaa atccaaaccc gaacccgaac ccgaacccga acccgaaccc
      121 gaacccgaac ccgaacccaa acccaaaccc aaatccaaat ccagacccga ctcccgatcc
      181 aacgccgaca cccgttccgg agaaacgcat cacgccttct acggcagctg tactcaatat
      241 ggcagcaaca ttaccgttgg tatttgatgc tgagctaaac agtattcgcg agcggttgaa
      301 cataatgaaa gcgagtccac acaacaataa tgtctggggg gcgacgtata acacccgtaa
      361 taatgtcacc accgatgcgg gtgccgggtt tgagcagacg ctgatcggaa tgacagtggg
      421 gatcgacagc cgtaatgata ttccagaagg aattgccacg ctgggcgctt ttatgggtta
      481 ttcccattca catatcggtt ttgatcgcgg aggtcatggc agtgtgggca gttattctct
      541 gggcggctat gccagttggg aacatgaaag tggtttctat ctggacggta tcgtgaagct
      601 gaaccgcttt gaaagtaacg tagccggtaa aatgagcagc ggtggagccg ccaacggcag
      661 ttatcgcagc aacgggctgg gcggtcacat tgaaaccggg atgcgattta ccgatggtaa
      721 ctggaacctg acgccgtatg catcgttaac ggggttcacc gctgataacc ccgaatatca
      781 tttatccaat ggtatggaat cgaaatcagt cgatacccgc agtatatatc gtgaactggg
      841 cgcaacgctg agttacaaca tgcgtctggg gaacggcatg gaagttgagc cgtggctgaa
      901 ggcggctgtg cgcaaagaat ttgtcgatga taaccgggtg aaagtgaata atgacggtaa
      961 ttttgtcaat gatttgtcgg gcagacgtgg aatatatcag gcaggtatta aagcctcatt
     1021 cagcagttcg ttaagcgggc atctcggggt ggggtatagc catggtgccg gtgtggaatc
     1081 cccgtggaac gcggtggctg gtgcgaactg gtcgttctga ccatcaacga ttaaactgcg
     1141 cttcggcgca gttttcgttt acagggtgtt aaaaggaaaa attctgcggc aaaaaaagcc
     1201 cgccagttac ggcgggaaac ctcatcctat gggagaacaa tgaataatga aattgcgggg
     1261 ttatcatcac ccagtatacc catactaaca ataaggttat ttactcagcc aggtataaac
     1321 aatttgatct gtgtgggggg aacggcatta aggtgtaaag gggggaagca atgagtagca
     1381 tcagcaagaa tactcgccgc tcttatcaaa cgtggatgag ggggatgaaa aacgcaaggc
     1441 agagatgact ctgccttgaa gagaaatgcg cttttacagc gggcctattt cagctcttct
     1501 gcttccggca aggtcacgtt cagctcaaga atagaaatat cgccatcttt ttgctcaagc
     1561 tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc aagaatatct
     1621 ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc agcaacaata
     1681 atctgcagcc gttcttttgc aatgttggcc gtgtttttct tccgcgagag aaagaaatcg
     1741 agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt ctcttcttca
     1801 atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc gtaggcttta
     1861 cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc gcgcaatact
     1921 gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag cacatcttcc
     1981 atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc
     2041 tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag aatgcctaaa
     2101 atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat ggcttcgtct
     2161 gcaaaataga gtgccattag cgcaccggtc tcaatccctg ccggggagtc gcaaacaata
     2221 aattcaaagt ccatcgcttt cagatcatca agaactttgg cgaccccttc acgggtgagg
     2281 gcatctttat cgcgagtttg cgatgccgga agaatataga gattttcagt acgcttatct
     2341 ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg
     2401 acccgacgtt cacaacccat aatcaggtcg agattacgca ggccgatatc aaaatctatc
     2461 acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc
     2521 ttaccaacac cccctttgcc cgaagtaaca acaataatgc gtgccataga aattccttgt
     2581 taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg
     2641 ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca cctgcgatgg
     2701 acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc
     2761 ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg gcaatcaatt
     2821 cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca taaatacgct
     2881 gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtt gtattttgcg
     2941 ctggagcctg cggtgcggga gctggacgcg gcgctttttc ctttccttcc gtcaggatag
     3001 gcagccccat tttttcaatt tcggctttaa gttgcgcatc tttgcagcca cttacgccaa
     3061 taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt accgggtctt
     3121 ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg gcctgagcga
     3181 ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga tgaaccacag
     3241 ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg gccttactca
     3301 attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag gcatgttata
     3361 gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag tctattctgt
     3421 gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc attgccgtcg
     3481 tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg acagcatctc
     3541 caaacctgtt taaatatcca gtattgaggc aagtgtattt tttcaccacg ccaggctttc
     3601 ttaactttcc attttttgcg gcatcattaa agaacagata cccgttatct taatatccgc
     3661 cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt caggtcatcg
     3721 taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc ataagtgttg
     3781 cacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata tcggattgtc
     3841 aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg gacttcaatc
     3901 tcctgcgtgt ggcgttgttt gcgacccgta tatttgtgtt aactcagatg gcatttcccc
     3961 agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat cagtacaagg
     4021 ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt
     4081 atgtcgtgat gatcgttatt ttggtgtgga tggtaagcgg agtggaaaaa tcaatcaaac
     4141 caccacaaag atgttattta tgtgtcgtga gtgatgtctt gtttacatta tgttctattc
     4201 gtcattaacc tcttgagcga cagagaggag tggggcgtta acgtttatga cggggagagt
     4261 ccccgtcggt tgacatttac ttaatttgaa tatcgacgtt atatttttta gttttgtttt
     4321 tacgggcgtc gtttcgagtt tgcagcactc gcagctcata tttgccagaa gcagggagtg
     4381 agtattgtcc gtgactatcg agttctgggg aatatctgga taaatcaaca gaatcatcga
     4441 ttcctggccc gaagaggtaa gtatcagcgc cttcattaga gatactcaca tgcactttct
     4501 gacctttttt ggcataaaag gtatatgtat cgtaatcgta tccctttatt tcgccggagt
     4561 actgagcgct gctatggcct tttctgaact caacattgac attcttaccc gcagcaaagc
     4621 tcgtggaggt tagcagtgca agcaataata cagccttact gatgctcttg attttcatag
     4681 gtagtctcct gttcagaatg agtacgatga actgttataa tataacaatt catcacagga
     4741 gaattccagt ggctttcctg tgttcggtta aatcccggcg cggcattttt gccgggggat
     4801 cacttattaa gggcgcatgg actgaacaaa ccgcagaaat ctacagctgg ctggcttgtg
     4861 gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga gtaaaagtat
     4921 gttttgtgtg atttatcgaa gcagcaagcg tgaccagacc tatttatatg tcgaaaaaaa
     4981 agacgatttt tcgcgtgtac ctgaggaact gatgaaaggt tttggtcagc ctcagttagc
     5041 gatgattctg ccgctggatg ggcggaagaa actggtcaat gccgatattg aaaaggttaa
     5101 acaggcatta accgagcaag gttactattt gcagttaccg ccaccacccg aagatttgct
     5161 gaagcaacat ctttccgtca tggggcagaa aacagacgac acgaacaaat aaccgatatc
     5221 cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg tgaggggaac
     5281 gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt gagtaaagta
     5341 gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc agtgcccgaa
     5401 gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca gccattggcg
     5461 atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtatt gattggcgcg
     5521 acgctgcgtc aggctacgga agagcatgtc cgtaaagcca ttgccggtta cggcgtggcg
     5581 ctcgatttga cattgcgtga tgttcaggga aaaatgaaga aagccgggca gccgtgggaa
     5641 aaggccaaag cgtttgataa ctcttgtccg ctttccgggt ttattcccgc ggcggaattc
     5701 accggcgatc cgcaaaatac aacgctgggc ctgagcgtaa acggcgaaca acgccagcaa
     5761 ggtacgactg cggacatgat ccataaaatc gttccgctga tcgcttatat gagcaagttc
     5821 tttaccctca aggccggtga cgttgtgctg acaggcacgc ctgatggcgt cggcccgttg
     5881 caaagtggtg atgagctgac agtcactttc gatgggcatt ctttgacaac tcgcgttttg
     5941 taatactttt tgccgcctga aagcggcggc aaaacttgca tcgctgtgcc agactggtta
     6001 taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt tctggcaaag
     6061 taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg gttgcggtca
     6121 gtgttgcctg cataaactga tggatgaaga caccgacgaa atctacttca ctaacgtcgc
     6181 ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc gtttcgagtt
     6241 tgaacctgac tgcattaaat taacccgtga aaatctgcca acattcgaat ggctgccaat
     6301 gacctgcgct tatcgcttgc tggcggaagg taaagattta cctgcgtggc atccgctgct
     6361 tactggttcg aaagcggcaa tgcatggcga acgtatctct gtgcgtcata tcgcagtgaa
     6421 agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact gggcgcagtg
     6481 ataaatttaa tagatgttga tgggggcttt cttaatatac gtctggatac tcgccatttc
     6541 gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc ttattatcaa
     6601 caggtaactc tcaatcttta aaaaacggct gtgtttttaa atgtgataaa ccttatacct
     6661 ggagtacatg gagagaataa tcgcttatca gacttcaggt acctcaaaga gtgtcttttt
     6721 accgtgtctt ttctgatact cattacaggt gttaatcatt tttttggccg cttcttttag
     6781 caaagaaagc attaaatcat cataatcaac gtagaatctg gttgtttcag tttccgtttt
     6841 tatctccccg atggcggcta tttcggtggt taatttcaat ttggcggcat cgatatcttt
     6901 attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag attttaactt
     6961 gttcttcaat tctgggatca gtttcccttc aactacgccc gcagcaatag aataggaaat
     7021 gattaatcca aatggaccgg cgacgacacc ggctgcggca ccggcatacg cttccttcct
     7081 gattttatct acctgtgact ggaaatagct gcttttttct gaaaaatcat tggttaactg
     7141 gctatctaac gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc ttaccagcag
     7201 ggatttttgc gcttcattca gcttcgtgat gccgtcatcc agtaccttaa tgagaatgtc
     7261 tttctgggcg gatgctttct tctcattgta ctcatcaaat agcaaaatat acgctgcgag
     7321 caattgcgtc gcaacaccac accattcata caccgtttgg gttgcttcaa aatacttatc
     7381 ctggctatcc ataagtaagg ttttaatatc gccaactaaa acggaggctg cctgtgaata
     7441 ctcctgttta aagcgactta actcttttat ggtttcatcg aaggtctgcc aggggatgac
     7501 ctgatcgaga tatttattat aaaggtctaa tgctccatct gcggtttcga ttgcgttttt
     7561 aactacctct accgttttat ctgcaacgat ttcagtcata atcattcgcc tctttaaata
     7621 tataaattgt aatgaaactc ctgttttaca actattaatg aattttactt catctaattc
     7681 atagttaggc gggcgagatg cgtcaatgtc tttatttcta ttaatatgat aaatatcaaa
     7741 caatgtttaa tgtcattatg gcgaatgctt ctattctatt tttagccggg tgatattttt
     7801 catttctgct ggatgagcgt cgtcgccaga aggccgcgtg agcacaggat aagagaacga
     7861 aaaatcagca gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg
     7921 caagaacaga ctactgtata taaaaacagt ataacttcag gcagattatt atgttgttta
     7981 tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt
     8041 gtggctttcc ttcaccggcc gcagattacg ttgaacagcg catcgatctg aatcaactgt
     8101 tgatccagca tcccagcgcg acttacttcg tcaaagcaag tggtgattct atgattgacg
     8161 gtgggattag tgacggtgat ttactgattg tcgatagcgc tattaccgcc agccatggtg
     8221 atattgtcat cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga
     8281 cagtgcagct tattcccatg aacagtgcat attcacccat taccatcagt agcgaagata
     8341 cgctggatgt ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga tgtttgccct
     8401 ctgtgatgta aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg
     8461 taaaccggtg gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc
     8521 aaaggcgttg ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgccg
     8581 ctgtggcgtg gtttgcttta gcagtaacta tgagctttac gccgatatga gcaatcgggt
     8641 gatgtcgacg ctggaagaat tatcgccccg cgtcgagatt tacagtattg atgaggcatt
     8701 ctgcgatctg accggtgtgc gtaattgtcg cgatctgact gattttggcc gagaaattcg
     8761 cgcaacggtg ctacaacgta cccatcttac tgttggcgtg gggatcgccc agaccaaaac
     8821 gctggctaag cttgccaatc atgcggcaaa aaaatggcag cggcagacgg gtggggtggt
     8881 ggatttatca aatctggaac gccagcgtaa attaatgtct gctctccccg tggatgaagt
     8941 ctgggggatt ggacggcgga tcagcaaaaa actggacgcg atggggatca aaaccgttct
     9001 cgatttggcg ggtacagata tccggtttat ccgtaaacat tttaatgtcg tgctcgaaag
     9061 aacggtgcgt gaactgcacg gtgaaccctg tttgcaactg gaagagtttg caccgacgaa
     9121 gcaggaaatt atctgttccc gttcgtttgg tgaacgcatc acggattata cgtcgatgcg
     9181 gcaggccatt tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagtg agcatcaata
     9241 ttgtcggttt atttccacgt ttattaagac gtcaccattt gcgctcaatg aaccttatta
     9301 cggcaatagc gcgtcggtaa aactgctgac gcccactcag gacagcaggg atatcattaa
     9361 cgccgctacg cgatctctgg atgccatctg gcaagcgggc catcgctacc aaaaagcggg
     9421 cgtgatgctg ggagatttct tcagtcaggg agtcgcgcag ctcaatttat tcgatgacaa
     9481 cgcaccgccc cccgggagtg agcaattgat ggcggtaatg gatactctga atgctaaaga
     9541 gggcagagga acactctatt ttgccgggca ggggatccaa caacaatggc agatgaagcg
     9601 agctatgctt tcaccacgtt atacaacgcg aagttctgat ttactgcggg tcaaataaac
     9661 atagcggcag gaaaaaagcg ctcccgcagg agcgctgaat ggattagcga ccgaacaggt
     9721 cacgtttttt cgctttaaac ggctgggaaa tcaccaccag caccgcgaca agcaggtaag
     9781 cgataaaaat accgagcaac cactgcggca tttccagacc taaaaaatcc cactgacgct
     9841 cggcgcaatc gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt
     9901 ccgggaaacg aaccataaaa tcacaggtcg caaacggcga aggatagagc tgaagcatgg
     9961 tgtgctcgta agttaactgc acaccgcgga acgcactata caaccagata accatcgcta
    10021 cataacgcag cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga
    10081 ataatgcgca gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga
    10141 accacagtgc cgtcagttcc agtgccagag cggtaaacgc catcaacagc cacgcgcccc
    10201 ggccttgtga acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga
    10261 gtgcgcagtt taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca
    10321 attggacaat tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat
    10381 caaagcgttg ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac
    10441 tcgacgcaaa gtagtccgac gagtgtcagg acgagggtgt aaggcagggc catccacacc
    10501 atgcggccat aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac
    10561 gcagcctgac cgttcggcgt agcgacagag ggcagattgg taccggtatt aatggcgacc
    10621 gccagcagct cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg
    10681 ttgatataaa tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa
    10741 atatagaaca gcgacagctg agcatgttcc gatgcctgca acacaaactg aataatcggc
    10801 gaaaacagtt gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg
    10861 gtgaatggca gagattcggt gaaggcttta ccgatagcat gttcatcggt gacaccggtc
    10921 aatgatgtcg ccagaataat gactgacaaa ccaatcaagc caacttccgc cagatgcaac
    10981 gccagcgcag tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc
    11041 tgacgggtac gctggtggcg gctttgatcg tcaaactgtt gcagtacttc gcggactttc
    11101 tccggcagcg tttcaccata gccaaaccaa cacagcttct ctaccagcag gcaggttaac
    11161 aggccacaaa tcagaaccgg aacggtcact ggcgacatgc gcaggaagaa atcgccaaaa
    11221 tgccagccag ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcattacg
    11281 ccgcctaatg cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac
    11341 tgttccagaa ccactttgta atgcttgtcg atatgactat cgtcttgcag gtcggtatct
    11401 tcggtacggg aagaggctac gcggtgataa atcccataaa aaccgactgc aacgctgatc
    11461 accaccgcca cgacggttaa ggcatcgagg aacgcggaga ggaacgccgc cgccacgcaa
    11521 aaagagagcg acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac
    11581 agcaacagct gtttcataaa atagataccc gccaccataa acatcagtaa cagcaagact
    11641 tcaagatttg ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc
    11701 gcttcgatag ccaacaggcc accggggagt agcgggtagc atttcagggc catcgccaga
    11761 gtgaaaataa attccgctac cagcaaccag ccagcgacga aagggctgat gaggaaaatt
    11821 aacggattta cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc
    11881 aaaaaattgc gccataacgc gcggccccag gagatctcca tgatggtttc ccttacctta
    11941 caaataatca atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg caggagtgag
    12001 gcaagtcttg atagtcaagg gtaaagagat gcggaaaatg aagccttgat cccttttttc
    12061 ttctttttgt ctgctatcag cgtagttagc cctctggtat gatgagtcca actttgtttt
    12121 gctgtgttat ggaaatctca ctatggtcat taaggcgcaa agcccggcgg gtttcgcgga
    12181 agagtacatt attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc
    12241 agaacgtgaa ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca
    12301 acgtctggca cgagatggct ggttgaccat tcaacacggc aagccgacga aggtgaataa
    12361 tttctgggaa acctccggtt taaatatcct tgaaacactg gcacgactgg atcacgaaag
    12421 tgtgccgcag cttattgata atttgctgtc ggtgcgtacc aatatttcaa cgatttttat
    12481 tcgcaccgcg tttcgtcagc atcctgataa agcgcaggaa gtactggcaa ccgctaatga
    12541 agtggccgat cacgccgatg cctttgccga gctggattac aacatattcc gcggtctggc
    12601 gtttgcttcc ggcaacccga tttacggtct gatccttaac gggatgaaag ggctgtatac
    12661 gcgcattggt cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggtttcta
    12721 ccacaaactg tcggcattgt gcagtgaagg cgcacacgat caggtatacg aaacagtgcg
    12781 tcgctatggg catgagagcg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga
    12841 tttagccatt caggggcgat aatcccttcc gtttaaaaag caaacccctc aaacgagggg
    12901 ttttgttgtt tttacagatt tcccattctc ggcgggcaac gttccagcaa ctcgacgctg
    12961 ccgtcttcgt tttgctgttc cagcattaca tcaaatcccc acaggcgatg cacatgcttc
    13021 agtacttctt tgcgcccccg atctagcggt gcgcgattat gtggaatgta acgcagcgtc
    13081 agcgaacggt cgccgcgcaa atccacgttc cagatctgaa tattcggctc cagattactt
    13141 aaattatatt gagacgataa ccggttacgg atctcccgat aaccttcttc attatgaata
    13201 gcggaaatct ccagataatt atgccgatcg tcatccagca cggtgaagaa gcggaaatca
    13261 cgcatcactt tcggtgacag gaactggctg ataaaactct catctttgaa atcacgcatc
    13321 gcgaaatgca gcgtttccag ccagtcggaa ccggcgatat ccgggaacca gtatttgtct
    13381 tcttccgttg gcgactgaca aatccgttta atatcctgga acatggcgaa cccgagggca
    13441 tacgggttga tgccgctgta ccacgggctg ttatagggtg gctggaagac cacattggtg
    13501 tggctgtgca aaaactccag cataaaacgt tccgttactt tcccttcatc atacagatgg
    13561 ttaaggatgg tgtagtgcca gaaggtcgcc cagccttcgt tcatcacctg agtctgtttt
    13621 tgcggataaa aatactggct caccttacgc acaatacgca gaatttcacg ctgccatgat
    13681 tccagcagtg gcgcattttt ctccataaaa tagagcaggt tttcttgtgg ctcggacgga
    13741 tagcggcgcg cttcagcaac cgttttctct tcctcgcgct tcggcagggt acgccagagc
    13801 atattgacct gactttgcag atactcttcg cgacttttct gccgggcttt ctcttcttgc
    13861 agcgagattt tttgcgggcg tttgtaccgg tccacgccgt agttcatcag cgcgtggcac
    13921 gagtccagaa gccgttctac ttcatcaacg ccataacgtt cttcgcactc ggtaatatat
    13981 ttacgggcaa aaatcagata atcgacaatc gaactggcgt cggtccagct acggaataag
    14041 taattgtttt tgaagaaaga gttatgccca tagcaggcgt gtgccatcac cagcgcttgc
    14101 atggtaatgg tgttttcttc catcaggtaa gcgatacacg ggttagagtt aatgacgatt
    14161 tcataggcca gtccttgctg accgtgctta tacagccgtt cagtctcgat aaacttttta
    14221 ccgaatgacc agtgcggata gttaattggc atgccgacgc tggagtaggc atccatcatc
    14281 tgttctgagg ttatcacttc aatctggtgc gggtaggtat ccagccggta gagtttcgcc
    14341 acccggtcta tctctgccag ataaacatcc agcaggtcga acgtccagtc gggtccatcg
    14401 ctcaaacgtg tggtgtcctt attcatagaa tcgatcgtcg ccatacgcgc acctcattgt
    14461 tgtcggcgct ctctgtgtgg agcacctcat ttcaagcata gaacacctgt taaaaaccgc
    14521 gtcgccggag aatttttttc tttgcgattt cttattatca gagtgccact aatccgcttc
    14581 tgaacggaat tttatgctgg ataaaaaggg cgttcagcag gagatactaa agacgtcata
    14641 ttgccgcaga gtcagggaga tgtgagccag ctcaccataa aaaagccgta tgttgaataa
    14701 tattttcaac tgagttatca agatgtaatt agattattat tcttttactg tatctaccgt
    14761 tatcggagtg gctatgcgag ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc
    14821 ctggtactta aatcaggcag gacatgaggt caccgtcatt gatcgagagc cgggggccgc
    14881 cctggaaacc agtgccgcga atgccgggca aatctccccc ggatatgcag caccgtgggc
    14941 ggcaccaggt gtgcctttaa aagcgattaa atggatgttc cagcgccatg cgccgctggc
    15001 ggttcgtctc gacggtacgc agttccagtt gaaatggatg tggcaaatgt tacgtaactg
    15061 cgacaccagc cactacatgg aaaacaaagg gcggatggtg cgtctggcgg aatacagccg
    15121 tgattgcctg aaagcattgc gcgccgaaac caatattcag tatgaagggc gtcagggtgg
    15181 gacgctgcaa ctgttccgta ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt
    15241 gctggaagat gctggcgtac cgtatcaact gttggaatcc agccgcctgg cggaagtgga
    15301 gcccgcgctg gcagaagtag cgcacaaact gacggggggg ctccagctgc ccaatgatga
    15361 aaccggagac tgccagctgt ttacccagaa tctggcgcgg atggcggagc aggcgggggt
    15421 taaattccgc tttaatacgc ccgttgacca actgctttgc gacggcgagc aaatctacgg
    15481 cgtgaagtgt ggcgatgaag tgattaaggc cgatgcatat gtgatggcat ttggttctta
    15541 ctcgacggcg atgctcaaag gcattgttga tattccggtg tatccgctga aaggctactc
    15601 gctgactatt ccgattgcgc aggaagatgg tgcgccggtg tctactatcc ttgatgaaac
    15661 ctacaaaatc gccattaccc gtttcgataa ccgcattcgt gttggcggaa tggcggaaat
    15721 tgttggtttt aacaccgagc tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg
    15781 cgatctctat ccacgcggcg gtcatgtcga gcaggcgact ttctggacgg gtctgcgccc
    15841 gatgacgcca gacggcacgc cggttgtcgg gcgtacacgc tttaaaaatc tgtggctgaa
    15901 taccggtcac ggcacgctcg gctggacgat ggcttgcggt tccggtcagt tgttaagcga
    15961 tctgctctct ggtcgcacgc cagcgattcc gtatgaggat ctaagcgtag cgcgctacag
    16021 ccgtggattt acgccatcac gcccgggcca tttacatggc gcgcacagct aaggaaacga
    16081 gatgacccgt ccgatacagg ccagcctcga tctgcaggca ttaaaacaga atctgtccat
    16141 tgtccgccag gccgcgccgc acgcgcgcgt ctggtcagtg gtaaaagcga acgcttacgg
    16201 gcacggtatt gagcgtatct ggagcgcgct cggggccacc gatggctttg cattacttaa
    16261 cctggaagag gcaataacgt tacgtgagcg cggctggaaa ggaccgatcc tgatgctgga
    16321 aggatttttc catgctcagg atctggagat ttatgaccag caccgcctga ccacctgcgt
    16381 acacagcaac tggcagctca aagcactgca aaatgcgcgg ctaaaagcac cgttggatat
    16441 ttatcttaaa gtgaatagtg ggatgaatcg gttgggcttc cagcccgatc gcgtacttac
    16501 cgtctggcag cagttgcggg cgatggcgaa tgttggcgaa atgaccctga tgtcgcattt
    16561 tgccgaggcg gaacatcctg atggaatttc cggcgcgatg gagcgtattg agcaggcggc
    16621 ggaagggctg gagtgtcggc gttcgttgtc caattcggcg gcgactctgt ggcacccgga
    16681 agcgcatttt gactgggttc ggcctggcat tattttgtat ggcgcttcgc cgtccggtca
    16741 gtggcgtgat atcgccaata ccggattacg tccggtgatg acgctaagca gtgagattat
    16801 tggtgtccag acgctaaaag cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg
    16861 cgatgaacag cgaatcggca ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc
    16921 gcctaccggt actcctgttt tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc
    16981 gatggatatg ctggcggttg atttaacgcc ttgcccgcag gcggggattg gtacgccggt
    17041 tgagctgtgg ggcaaggaga tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg
    17101 ctatgagttg atgtgcgcgc tggcgttacg tgtcccggtt atgacagtgt aacttgttgt
    17161 aagccggatc ggaggcagcg tcttctggtt gcgaaaaaat catccatccg gctggtcagc
    17221 aactgtagtt gttgatgtga cagagccatt tcccatgata gtgtccatta caatgatgga
    17281 cactatttcc ccggaacctg agttcaccgc acaggcgttc tacataaaac gcttacgttt
    17341 cattgttgac tcgaactcga cttcagataa atcacgcttc acccttgatg cagattcggg
    17401 ttcatgttca gaatgttgat taacaggctg cgagtcctgt tgtctttcca gaatcaacag
    17461 gcccaactgc tgattcagga gctccaggtc ctcctgtacc ttcacgcgct ccaggagtac
    17521 cgggtcatcc aggagctcta gatccataag gtcatccaga tcctccagct tctccagctt
    17581 ctccagcttc atctggccct ccttgtgcaa aaagcccaaa aggtccttca ggccctgctg
    17641 ttttttcagg ccctcgacgg ctttgaacag cgtcggtaaa ttgtctttgt cccacgggtt
    17701 accattacag tcaaacacct tcaggctctg gggctgtggc acctgtaccg ttttaaactt
    17761 gttttctcca cagtagtaaa ttattagttt gggcggcagc cgtggtgcag agatcaaatc
    17821 gttactagaa cagtcaaata ctttcaggcg agtcaagttc tccagcatct catcaggtat
    17881 tgagtgaata ttgttgccgg cacaggatag tttatacagg tactggggca tcgacggcgg
    17941 taaaaattgc aagaagtttt cagaacagtt aatcactctc aggttagtcg acagctcagg
    18001 cagccagata atacagtttt catagcactt cagtaccatc agttttgagg gctgcacctt
    18061 cggtaacaag atcaagacgt tatctgtaca gtcaagcacc agcagccact ttggcatcac
    18121 cttcggtaac gcggtcaaca cgtttttact gcagtaaaat tcaatcagat gtggaggcaa
    18181 attctcaggg agtgaagtca atcccaggtt gctaagattg agcatactct gtcttgtaat
    18241 gagacattcc ttcattctgc gcaatgcttc tgcccggttt tcaatccggc ttgctggcag
    18301 gttttcttcc aggatatttt ccttgatttc ttccagactt tctttcaggt cttcttgggg
    18361 aatttctgac tgtttgtttt gctggttttc ttccaggatt tcgtacaggt ttttttgcag
    18421 gttttcttgc aaaccatccc gctccatagc ccattggcac cattcggcga ccatcatgaa
    18481 ctcgttatgt tgcggattcg gattcaggcg ctccaacgtg atgatggtat tgtctaccat
    18541 atgtattttc acttcgcccg cggtactgag gtcaatgatc tgccggtggt catcacccca
    18601 gctaaatatt tgcgggccga tatacttcat gttccgcaaa atttcattct tgagttccac
    18661 cggcaggaag aactgccctg gtgggcgtac gcctgggtta acggaagatg ggggaatgcc
    18721 gggggcgaat gggcggatgt ctttaatgct ggccataatg ctacgtatcc ttgaaataaa
    18781 tatcctctgg cagcgccgga gacgtgaatg tggtcaccgc aaaggtgcca caggacgtca
    18841 tttgcgctga tataacgact catcgttgac tttttccctt ggtcagaaat gccgtgtcgg
    18901 gtctggtaca tgctgtatta cggcccaggc agctctgacg tattaaagaa aatacgagag
    18961 cgaacgaccc ggaaataatc gttattaagc aaaggaataa ctataagaaa tcgctcatgc
    19021 atccggtgct acaggcggat gaaacgccac tacagttact gagcagtctg aaacgcgtat
    19081 tgtgtgggct acgccagtgc ggcaggttgc gtgggggaat atgttcaggc gatgtcaggc
    19141 tggggcccct gatggttgac ggggataccg gtaatgcggg gctggttatt cttcacctgt
    19201 tgcagtcaga agaagccatt atgctgactt tcttgagccg gcgttttgag ttgattagcc
    19261 agagcctgca atggtttgtc atccataatt taaccttctt ctgatgttgg tatgagcata
    19321 tcaaaatcag gaaatgacaa gtttatatgc aggctcaggc tgcgttcgca aagcatcgca
    19381 tccggcgata gatgctgtat taaatttaag attcagcttc ctcttccgcc acccgtacgc
    19441 caatcttcaa cacttcatta tcttctttct cggccaccgt ccagatcatt ccggcaaact
    19501 ctacctggtc accgacaacc ggtgccgcgc ctaacaactg ctggacaatt tcacccagcg
    19561 tttgttgctt atcacgatat tctcgcccgt cttccaggcc atatatcagc gccacatctg
    19621 catatttggc gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg
    19681 gcggcgactg gctgaacagt ttaccgagcg ccgggagatc gcgttcccga ccaattacac
    19741 acaacacatc gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac
    19801 gaaacagtgc cgcaatacgc gtctcttttg gcatatgcag atcacgcagt gccgcgccca
    19861 cgcaccattt atcggcactc aactgataaa caaactgctc ccacggattt tccggatgaa
    19921 tatccagacc aacgcgtgac accggacgtc ccactggcgg aacgaccact ttggcttttt
    19981 tcgccgccca cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg
    20041 cgacattaaa gaacagtcgc gcattctcca gccccgccat catcgggaac acggccagga
    20101 tgattggcac cgcgccgcgt aatcccaccc agctgataaa cacgcgctca cgcagattaa
    20161 aaccacggaa cggtagcaat ccggcaaata ccgaaagagg acgagcgaag aatatcatcc
    20221 atgcggacaa aatgagcgcc ggaatagcaa taggcagcag atcgcttggg ttaaccaata
    20281 gccccagcac caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca
    20341 ggatgccata gcggttgcga atcgggcgat tacccagcag aaaaccgcac agataaacag
    20401 ccagaatacc gctaccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta
    20461 atgccagcaa tggatataat ccggcgggca gggcgatgcg attaatcatt tgcagcagta
    20521 aataaccacc gccaagaccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacta
    20581 cgaacatcca actgacactg ctttcatgtt gctggatcat cgcaatcagg gtaatcgtca
    20641 gaaagaccgc cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt
    20701 cgttaagccc cttaccaccc agcagagaaa agaccgctgc agcatcggtg gagccgacga
    20761 tagcgccgat taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc
    20821 cggttaaacc agaggtgata agcacgccca gcgtcgccag cgacagggcc ggtcctaacg
    20881 ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagcg
    20941 ccaggttact taccatgtag gcgaaggggt aattatcaaa cgggatacct ccgacgccat
    21001 cgactcccgc cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg
    21061 aagaaaatga actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc
    21121 taattattgt tgtggcatcc aacggtcggt tactcctgat tacgctgtct cttatataaa
    21181 ccctaccata ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac
    21241 attttaagaa caggatgacc gctaatcgtc aactgagtgc cttcccgggt gttagtaaga
    21301 atggcatgtg cgcccaacgg cagcgtaacc gtgcgtggtt catgaccaaa atcgaggccg
    21361 gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa
    21421 cctgcgtcat aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga
    21481 cgtggcaaaa ttccagcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc
    21541 tcgttaatat cttccagcac cagaataccg ttttcgattt gcggcatcca cggtgtacca
    21601 atcagtgaaa tcagcatcgc gagattgcct ccccataacg tgccttcggt ctgacatgtc
    21661 gggccttccc cttgccattc aatggtgaag gtttcattgc gtaacgccag ccagaaatgg
    21721 tgctcggtaa aggcgttcag ttcatccgcg ccaaagtttg ctaccagcat ggggccgcta
    21781 aaggtgatga cattgccatg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt
    21841 ccgcaaatga gcaacgggtc atgttgttgg cgagccacca gtgcctgcca gtcaatatcc
    21901 gccagtaaac gactggcacc gtaaccaccg cgtacagcca gcacgatagt gttgggggta
    21961 gtcagtctgg caagggaatt gagatcttcc agacgctccg tttccgtacc cgcaaaacgc
    22021 tcacaacgac gggcaataac atcgacgtta ttgacctgat gccccgcgtc ggtaaggcgc
    22081 tggataccac gcagcgcggc gtgctgttta atgcagtaac ccgatggggc aattaagtga
    22141 aacagagaca tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag
    22201 gtgtgccgtt gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga
    22261 gatggtttgc ctttttgatt gtgttattag caggttgttc atcaaagcat gactacacga
    22321 acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc
    22381 agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat cacagcgatt atcgctatcg
    22441 aatcgggtgg taatccgaat gcggtgagta aatcgaatgc cattggtttg atgcagttaa
    22501 aagcttcaac ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga
    22561 ccagcgagct gaaaaatccg gagcgtaata tttcaatggg ggcggcttac ctgaatattc
    22621 tggaaaccgg cccgctggca ggtattgaag atccgaaggt actgcaatat gcgctggtgg
    22681 tgtcatacgc taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg
    22741 cgatcagcaa aatcaacgat ctggatgctg acgagttcct cgatcacgta gcgcgaaatc
    22801 atcctgcgcc gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt
    22861 aaatcagtcg cgcactttgt ccgctttctc ccgggcttcc cgctcgagag agaaaataat
    22921 ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg
    22981 agtggtgatg gtttcattct tgccatcaat cactttgcgc tcgctgattg agattaactg
    23041 ggcgtcaaag tgaaaaacac cccattgccc catgttgact tcaatctgag cgaagcgcat
    23101 gccttcgtgc aatccggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga
    23161 caaatcatac aggcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata
    23221 aggcggatgg agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accacaaggt
    23281 gggaggcggt acggtaataa atgccggaag ctgcaagtat tcactctgct gtagttgttc
    23341 aacagtaaat tcgactttcg cgccctgggt ttcggcggta atggtaatgt gctgcgcctt
    23401 taacacagcg ttgttatctt cggcctggct gccgaaatcc agcaccagtt tatccggggt
    23461 tattgccagt attttgctga tcagctgccc gccattccaa ctgatacgca aaggaattgc
    23521 ggctttgtgc aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg
    23581 ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg
    23641 tcacagttaa caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc
    23701 agccgacata cgcccagcat cgattccgcc cttgcctata cttagagcgt tgacgtaagc
    23761 atttcttgcg tccgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta
    23821 tttttggcct gatagccggc attatcgcca agctaatcat gccggggcgt gatggtggtg
    23881 gatttttcct gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga
    23941 ccatgtttgg cattggcggc tccatcagcg gttttaattt gcacagcttc ctggtggcgg
    24001 tagtgggcgc tattctggtg ctgggcgtat tccgcctcct gcgaagagaa taagattttc
    24061 ataaggcgga tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgat
    24121 acctgcccag aaattgcgac cttcttcaat aaacccttca ctgtaggcat attgggtatc
    24181 aaacaggtta ttgaccgacg cattgacgct gaagccatga cctaaggtgt aatcggctcg
    24241 aatgtgggtc accgcaaaac cggcggcttt ttgtgaaccg tcactgttgc tgtagctgga
    24301 ggaacgcgcc tcttccgaca gcgttacgct taacggctcc cacggtttga gagtcatcca
    24361 tgcggtcatt gtctgcgttg gcagatcggt tatcttgccg atgtctttgc gtttggcatc
    24421 agcgtggatc agggcgtagc tcaatcctac atccagtata ttgctgattt tccccttaat
    24481 accggcatcc agaccgctgt aatccaccgt gccgctgttc tgattttgaa tggtatcggc
    24541 atcgatattg tgcgagagga tggcatcact cacccggtta tagtaaacgc tgacctcaaa
    24601 cccccagtcg tgcgtgaagg caccattcca ggttaaatcc accccgcgcg cgcgttccgg
    24661 tttgagctgc gggttaacaa tcgctatctg gttatacgca ggtttggacg tggtatagcg
    24721 ttctttcagc gtcggaaagc gtgtgcggtc atagtacgaa agcgccagcg tgtcttcatt
    24781 ggcaaagtgg tatttcccca tcacctgcca gttaaaagct gactgattgt tgtcgtcata
    24841 atgggtgatg cttccatctt tctcatgttt tttcgcttct acgctatcgc gccagtcata
    24901 gctgattcca gccacgacat cgacattatc ggcagcagcc cattgatatt cactggcgag
    24961 cgaccaggta cgatcttcat agcgatcgta agcggcgtgc ggcgcacctt tttcccggtg
    25021 tacgtcatct ttccagttaa cggcaaacga cagcagatcg ttttcacgca catcggctgc
    25081 cagttgtaac ccggcaccgt cgctgtaatc ggaataatgg ctgtagctgc cttttttatt
    25141 tttcaaatca gccagcgagt tgtacatcat cagcgtattt tcaaaggtgt cgcgatacag
    25201 ccgacttttc agggtaaaac gatcgtttag ttgggtcgtt ccctgataat aaaaactttc
    25261 tttgtcatac tctggccact gccagtagcg tgatttttga ccactatttc cactgtatgg
    25321 cgggttatct ttttcaccat cctgcttaat gtaagtcaat gtgtattcat cgttttcgcg
    25381 tggtgtaaaa ccgagcttca caatgccgcg tttatcatca gccgatgaat taatcatctt
    25441 gccgtgtttg cctgcaatat cattatttac accatgcggc aggccgagaa aatcctgctt
    25501 tagctggcta ccgctgactt gcaaataccc cagatcgctg ctggcggcaa atgaagcatg
    25561 catatcgtag gcattgtcct ggctacggct ccatccctgg cgatatccca gatttgcttc
    25621 cagaggtttt gttggcttct gggtggtgat attaatggct ccgcccatct gattaggccc
    25681 ctgaagcagc gacgaatacc ctttggaaac ttcaactgcc cccaaattgt tggtcagaat
    25741 ccgcgccaga tcgaggttgc cgtcataggg aacataaatg ggcacaccgt cgaaatagac
    25801 cggcacctga cgactatcaa agccacgaac tttgacctgt tcttcgttgc ggctacctga
    25861 cttttgcagc accacgccgg ggacgacact taatgcctga gcgacattct gtttatccag
    25921 ggccttcatg gtgggttgat cgagaacggt ggtcgtcgtc gatgataccg gactggacca
    25981 tacggtcagc gtgtcactct cctgagccgc tacggcctgc tgggtaaagg caagtgataa
    26041 tgctgtgcag agataacgtt ttttgagtct catagaataa attccgttta acaaagaatt
    26101 aaatagaggc tgcgggttgc agactgactt taatgtcagc cggtgtcgcg taataaggcg
    26161 cggaggtaat aaaaaggcga atgccgcagt cgaggtaatt tttgagtgtt gtcagattaa
    26221 ttccgccggt cagagccagc gtgcagtggg gagccagcga cggtgctatt tgcgcaattt
    26281 cagttgcctg ttgtggacta aatttgtcga gctgaagtac gtctggttgc gcgcgtaacg
    26341 cggcgattgc ctctttcggc gtgtcggctt caacaactat tttcttctcg ggtgcgtggc
    26401 gacgtaactg attgattgcg cctgaccagt cctgattgtc atgaagaaaa tgacggtggt
    26461 tggcaaacaa taatatggtt tccgcacatc cggcacgatg aatcagtcct ccggcagcca
    26521 gaattgcctg cgaggccagt aagcgggtgc ccggaatcgc ttttcgggtg caggcgatat
    26581 tgccatcagg gtaacgttca cgaagtaacg ccagcatttg agcgagataa tcagaaacac
    26641 cgcaactcca ctccagcaca ttctggactg ccttccatcc ctgatgaagt gctgcggcat
    26701 taccctgcgc acggattagc cgctgacccg cgttcgcttg tgaaccgtcg ctgaccgcgt
    26761 catcaatggt taaccccagc gtggttaaca tcttacacgc gacagaaatt ccgctgacgc
    26821 agccgccctg acggtgaaaa aactctatat agccatgctg gtgtccaata tttaatgctc
    26881 gcgtggtcag gtcaccgccc tggatatctt ccagaagtaa cgcatcgatt tgtgcctgag
    26941 aaaggaaaat catcgcagtg cctccggctg cacgctatcc caccagatga gcgcccagtc
    27001 gcggctggca ggggcgaggg cacgagcatc ttgttgttga taccagcgat ataaccgctc
    27061 acgctcatcg tcgttaagcg cgcccagact ccagctgacg ttctgctcaa aacgttccca
    27121 ggtgctgtta tcctgttggc aattttgtcc acggataaaa tcaacgtgag cataaattcc
    27181 catctgatac aggacgttga gcgcaaagat atagttgggt aattcgatca cttcccgacc
    27241 agcagcccgc tgaatggctg gagagacgaa cgaggtgctg accagatgag tcgtatagac
    27301 gcgaagccgt gcctgattat tcagtttgct cattgcctgg cgcatatcag ccaccagagt
    27361 ggagcgggaa gccatggcaa tatcgcagcg tggcaggtca ctccagtcct cttcccaggc
    27421 gcgctggatc cagtggacat tgtctgcctt tagcgcggcg gcgcgacggg cggcaacctt
    27481 cagcatcccc tgactgtaat caacgccata aatcgtcgtg agtttgtcag ccagcgccag
    27541 cgatacggta cccggtccgc agcccatatc gaataaggtc tgcgcgcctt gcagatcaat
    27601 tttagcggtc agttgttgca ggtagctgtc cgtaggcgag gcgcaatttt ccgccatttt
    27661 ttccgcccgt tgatcccagt gatccggcgt tttttctgtg cggtgggcca gtttgagttg
    27721 ctgaaggtaa aggtctgcaa aatcaatatc gtcaatgagc atgatttttc cttagtgctt
    27781 aattgcagag aggtgatggt gaatctggtc ggcagaaacc cggtacaggg cagccagttt
    27841 gttcgttgtt aactgttcgg tcggtaagcc ttgtgttact cgtccatctg gctctacctg
    27901 gataatgctg tcggcaatgg cgttggcatg gagcggatga tgagtcgaca ttaacattgt
    27961 catgccgtta tttttgagct gcgccagcgt gtccagtagt tggatctgat gaccgaaatc
    28021 gaggctggag gcaggctcat ccagtaataa caagcgcggt tgctggacga gtgcacgggc
    28081 gattaaagcc agctgacgtt caccgccgct aagcgtattc cagcgtcgtg tcgccagatg
    28141 caaaatgccc agcttttcca gttgctccgt tgctttcagg cgttcttgct taccgggaac
    28201 ggaaaaagcg cctatggtgg gggccaggcc catcaacacc atatccagca cactaaaagc
    28261 aaaaatgccg tcgtgcgcct gcggcaccca ggcaatagcc tgcgcgcgct ggcgatggct
    28321 gagtgtctgc acagggaggc tatccagtaa gatctcgcct tttaataacg gtataacgcc
    28381 aagaatggag cgcatgagtg tggttttgcc gcaaccattg gctcctaaca gacaacaaat
    28441 tgttccttgc gacagggtga aggacacgtc gcgtagcacc ggatggcggg agtaacccag
    28501 agacgcatga cggacggcaa gcaaagtcac tggcgacctc cgcgtaacaa caaaaacagg
    28561 aaaaacggtg caccaacaaa agcggtcaga atgccaagtg ggatttctgt tgttccaatg
    28621 ctgcgtgcca gcgtatcggt aagcaggagc agaatcgcgc cagtacacat tgccatgggc
    28681 aacagttgct gatggttatg accggtcaac aagcgggcaa tgtgcggaac gaccaggccg
    28741 acccaaccaa ttatgcccgc tattgctacc gtgctggcgg tgattagtgt ggcgcagaca
    28801 attaatccaa agcgcagacg tgtcacgttc agtcccagcg aacgggcttc gtcgtcggaa
    28861 agcgtgagta aattcatgcg ccagcggaga aaaaacaggg gcaaggagcc tatgagaatg
    28921 atcggtgcag catagcagag atcgcgtaag gtcacggtgg acagcccccc cagcaaccag
    28981 aaggtaattg acggtaactg ggtgtaagga tcggccagcg tttttattag cgatatcccc
    29041 gctccgcaca acgttcctaa cgcaatgcct accagcacca gggttaagat cggatcgtgg
    29101 cgggtcaccc gacgcgtaat caaccagacg cccgcgacca ccatcagccc gccgcaaaat
    29161 gcgtacagct ggatgtaaac aatggataat ccccacagga ttgccgcgca ggcaccaagt
    29221 ccggcaccgg cggagacgcc taaaatatcc ggtgacacta agggattacg gaacattccc
    29281 tgataggtgg ttccggcccc tgccagcgcc gcgcccagaa acagcgcagc aagaatacgc
    29341 ggcacgcgga tttgccagaa gacaatttgt tcctgcacag gaacattttc ttgacgcaga
    29401 atgagcgcca gcagttgctg tatatcgaga tgataagccc cacttacggc ggcaaaagtg
    29461 atactgatag ctgccagcag caaaatacat ccgtttacgg ttctcattag ctcgccacca
    29521 atttttgata gtcggcgtca cttaatgggc aatgccagaa cagttgggcg taatgctgca
    29581 tgtcactttt aaactggcga ttgatggcgg gatccagcca ggcgtgaagt ctgcgtaatc
    29641 ccagaagacg gttgattccc ggcgggacat ccagccagcc aaagggcagg ccacttaaaa
    29701 ataagatgcg ttgttccgca accgctttca cgccctgcca gagaggatca cgacgaataa
    29761 aatctgcagt aacggcctcc tgaaccagaa taatatccgg ctgccagcgc aggagatttt
    29821 ccatggaaac ctgtgtcaga ccgtggcgat cggctatttg cgcgacgtta tgcaaaccta
    29881 ataattccgc cgcctcggta tgcaacgaac cctgtaagcc cgtttccagc cctcgaggac
    29941 cgcgcgcagc ataaaagctg aggttagcgg cgggtgaggt ggcgaatgcc tgagcttcac
    30001 caacgaagcg gctggcgaga ttggcttgtt ctgcggcgcg gtgctcttcg cctaacgttt
    30061 tgccaagcgt tgttaactgt tctgctgatt gttccagttt cccgttaagc aataaccagg
    30121 gtatttgtgt ctgttcgcta acctgccgtg cctgggagat ccaggtttca tccgtattgc
    30181 cgcaatcaac gaccaaatcg ggatgtaacg ccataagccc ttccagcgag agtgtgctgg
    30241 cgcgcccggc gagtcgtccc agcctgggga actggcgaat gtgctctggc aatggaatta
    30301 atgcctgacg ggcaaaatca aaagaggaaa aaccgaccat tttttctggc gcgacagcca
    30361 gcaatagtaa atcggcgggc gcgcctgcgc tgacgatgcg tgtaatgttt tgggcttcgg
    30421 gaagcgtcag tgaggcccag aatttgactg attgtgcaaa ggacggcaga tgatgcagga
    30481 acaacgtcga cgcaagaatc tgtgtaaacc tccgtctggt tagtgacata agtaatatcc
    30541 tgataattaa aaccgctata tacttacaca tatagcgaaa aagatcatgt ttgatgtcaa
    30601 aatttgtcta aagagacaga atgatttgct tgtttcttaa ataacacttt gttccacaag
    30661 aaaattgtca gggagaatgg cggagtgggg gaaaatataa atagaggtgt aatgattaac
    30721 aagttgataa aataaaatta aaatcttctc taataaaaga aaataaaggc atagaccgta
    30781 gaaagagtgg tatttggtat ttcgtggtga agaaagagca atgccaattt tatgcacgta
    30841 atggcattgc tcgatataca gtaaagttaa ggcgtaggtt ccgcctcatt ttgttttacc
    30901 ggctgtgttg ttgattcact gagtggacgc gtcgccggaa cattgtcaca cggttgctct
    30961 ttcgggcaga tcaaatccag cattttcagc gtcacgccat tggtccagcc aaagccatcc
    31021 tgtaatggat attcgccacc gccgccccct gttccggtgg tgctgacatc atatttttcc
    31081 accagctttt tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg
    31141 tccatcgcca cctctttttg cccgtagttt tgtaaccctt ctgtcgcgac ccactgcaac
    31201 ggtgcccagc cgtttggcgc atcccattgt tgcccacttt tcaccgacgt ggtgttcagg
    31261 ccgcctggtt gcagcagatg tgttttcgtc gccgtcgcca ttttgctggc gcgatctttc
    31321 gctgccgcat tgacgtacag cgggaacagg gcggccgcgg ttaactgatt gcgcacttta
    31381 tgacttttca ggtcgtaatc ggcataccag ccttgttgat cgttccacag gtatttttcg
    31441 atcccttttt gacgggcatt tgccagcgtt tcgtactggt ttgctatcgc gttatctccg
    31501 gcagctttgc tggcgcgggc gaggattttt tccattttaa acatcaggct gttcagatcg
    31561 accggtacga tgctggtggt gcgtaaggta tttaactgct gcgggttgtc catccagcgc
    31621 gagctgaaat cccagccaga cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca
    31681 ggtcgattcg gattgctttt ggcggtggca atatcttcca cccatgactc tggtcgtggc
    31741 gtatcgcgat cgtcccagta gcggttgaga agggtaccat cctgaagttt tacaacgcgt
    31801 ttttcctgtt gtccggcttg caggttttca acaccgtcca tccagtaagc atattctttt
    31861 tgcatttgcg gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc cagtaactct
    31921 accatcaggg caaagaaggg cggctgcgag cgacttaaat agtaactgcg gttgccgttg
    31981 ggaatatgac cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc cgcgacttta
    32041 tcccagtgac cgctttcggc aagtcctaac atggtgaagt aactgtccca gtaatatacc
    32101 tcgcgaaaac gtccgcctgg cacgacataa ggttccggca gcggtaacag agaatcccat
    32161 ttttcggtgt tttcggtaga acgcgttaat accggccaaa gtccgtcaat atgttcgcgc
    32221 agtgactgcc cctctggcgg aacatatttc tcgccctctt tcggcagggt aaaattgacg
    32281 ttaacgaaat ggcgcagatc aaatccgctc tggttttgct gcatccgata atcagcaagg
    32341 atcatcagcg gatcgctgtt cggcacggca tcagcaaagg ttttttggtc cggaaaaagt
    32401 ttggcgtttt gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggcgtt
    32461 accggtgttt cttctgcctg caccgatagc gcagcaaaac acaaaaagat gcaggctgga
    32521 attaacgcca ttttttgcgg gcgagaaggg gtgggggatt tcatcaatca ttctcctttg
    32581 gcgaaaccga ataaaacggt attcaagtat cagaaaacct tagttcagga tcgcactagc
    32641 tgcatgatcg aaatctcatt ctgcgaacgg tcaggcattt tagaaaaggg aattaaaggg
    32701 ataaataacg gcgaaataga gtgatcaaat aacgtctgtt tgttagccag ccagcggcgt
    32761 tgggttcgcg attagcgaag aaaaatcatc acaaattggt gaaaaatata acaggtagtt
    32821 aaaaacatcc gtgcagaata aattgccgga tgacatcaga acgatgccat ccgaactgtg
    32881 gtttaaccct gacggctgaa acgttgcgtt ttaacgtcca gcgttagcgt ttcttctggt
    32941 tgtatcgcat acagtttctc accctgctgg caaatccagc caatccccag ttcacgggcg
    33001 atcagggcgc tgtgggatag cggacttccg gcgctaaggc agataccttt tacaaccgcc
    33061 ggatccagtt gcagtattgt ggagggatag atgttctccg ccagtagaat agttggcgag
    33121 ttaaactgcg ggagttcttc tttcgtttgg gtcaggtgga ccagggtgcg atgcagaaga
    33181 tcgtccacat caatatagcg agcctgtaga tattcatcat ccagttgctg gtattgctgg
    33241 ctaagttctt taagaacttg ctgccaggca tattctgccg tgcaatgttc atgctgaagg
    33301 agttcgcttg ccgccgccag cagttccgga tcatctaaca gtgtatggtg accagaaaag
    33361 attgcggcaa tatcgtcaag cccgctggct tctgctttcg ctgttaacgt catcagatct
    33421 aataacgtga agtcaatggc ctggcgtaat cgttcttgtt cttcatccac ggtcagggtt
    33481 gattttgcct gtaccgtaca taaaaccggt tgataataaa aggctttacc cgaaacaggc
    33541 ggaacgggac gcagaatagg tggagcaact tcctccgttt caccaaagtt atcttcagcc
    33601 agctgacgga aagcgatcag tgcctcttca gcttctggcc ctttcgcaat caggcgcagc
    33661 gtatcgttat agcgaacttg tagtaacgca atctggttaa tactctccgg tgtgacgcat
    33721 ttgccgtttt tctccagcaa catatcggca ttaaatgtcg ataaggtata aaccagccgg
    33781 gaggccggac gtacatgcag gccgttacgg ttttttatga ccaccgccag agaacgggct
    33841 tcttcatcgt acggaggaca tgtgtcagag atttcagtgt tggaggacgg taaacccagt
    33901 tgttcacgtt tggcttccag cgcatgcatg gcgtcaaaga taactttgtc gatctccgcc
    33961 cccgaggccg cgctgaccgt tgctgccagt gtaccttcga ccaacggtgc agcacacaaa
    34021 cgtacttttg cggcgatctc gggagccagc aattccagcg cagtttcagc actcaataat
    34081 gcgctaccca tatccatcat gaccagcaca tggtcggcat cagcaacaga ttcgatggcc
    34141 tccatcactt tgacggcatc ggtaccaatg ggattttgtg gatcgtcaat tcccgcggca
    34201 atggcgattt tacaactatc actcattaac atctgacggg ctagttcacc gacaccttct
    34261 cccagtcggc tgctatgtga aactatgacc aggtttacca tcaccaattc cttactcttt
    34321 tgcggctaac gccaacattt gcatcataaa catcaccgag gtcgcaccgg ggtcctggtg
    34381 accaatactg cgttcaccga gataactggc gcggcctttg cgggcttgca tcgtaatcgt
    34441 actttgtgca gcggactcgg cgatgctact ggcagcttcg agcgccgccg gaacagagag
    34501 attttgctcg ctggactgac gtaacgattc caccaccggc acccacacat cacacatggt
    34561 tttatcgcct ggttcggctt taccgcgact gattacgccg tccgcgccat cgcggaacat
    34621 ctgataaagc tcttccagcg tcaggctttg ccgtgcctgg gtcgcctgtg cggcgcggat
    34681 aaagaaggta ccgaaaagcg gaccactggc accaccgacg ctggaaagta gcgtcatacc
    34741 ggtattcttg agaatgaaac cgatatcttt atctgcgata gcagggagtt tttccaccac
    34801 tttgctaaag cctcggttca tatttagccc gtggtcagcg tcgccaattt cgcgatccag
    34861 tccggtaaga tactcgctct cggtgctgaa aatatcgcca caacgagtga gccagttaac
    34921 aatttgagtt ctgctcagtg acattgctat ctccttattt accccagtta agggccgggg
    34981 tgtggaccgg ggcgtcccag agtgccagcg tttcgtcatc aactttcagt aaggtgattg
    35041 agaaaccggt catatccagt gaggtgcagt acgcgccaat taaattacgc tcgatagtca
    35101 atcccgcttg ctggcaacgt gtggtcaggc ggttatagac gccgtacagc tcagaaagcg
    35161 gagttgcgcc aagattgtta accagcgcaa tcacccgatc gccagactgg agcggttgtt
    35221 tggtttgtga ttcttcctgc cagctgcctt gttgataatc ccagaaacgc aaagtgcgat
    35281 ggtatgagcc attttccagc agggtgtcga acatttcatc gacggtttga tcaagagaag
    35341 agaaggggcg tcggtcaata cccggctcac catgaatgcc gacgccaaac tccatctcat
    35401 tgtccgccag ggtaaaagaa ggtttgcccg cggcaggaac ggtacaggca ccgagagcaa
    35461 tacctattga gtggccttga ttattcaact tacgccccag ttccgcacag gcgtccagtg
    35521 agtcgccacg ctccgccgct gcgcctacga gtttttcaat taatacggtg ttggcaacgc
    35581 cgcgccgccc ggcggtataa agactgtctt ttactgcaac gtcgtcatca atgaccacag
    35641 tggtcacttt tacgccgcta tcgtgcagta actcggttgc tgtttcgaag ttaagaatat
    35701 cgccggtgta atttttgata atcaacagta caccttcgcc gccatcaatt tgcatggcgc
    35761 attcaaagat tttatcgggc gtcggtgagg tgaaaatttc gcccggacag gcccccgaaa
    35821 gcatcccctg accgatataa ccacaatgca tcggctcgtg tccgctgccg ccacccgaca
    35881 gcagggcgac tttacctgca acgggggcat cagctcgggt gacatacacc ggatcctgat
    35941 gcagtgtcag cgatggatgc gctttcgcta gtcctgccag ttgttcgtcc agtacgtctt
    36001 gcacatcatt gatcaatttt ttcattattt tgctccagca attacggtag ggcatggatg
    36061 atgttcaacg acacggcgac cactgactgc cgatgaatcc attgtgcatc aggcaaggga
    36121 aaagaaaatt agcccaaatt atgattcata gtgaaacata tgctttaatg aatgttccat
    36181 attgaaactt ttacgtgtat taatacttaa aattgcgagc cggaacacct tttgtcataa
    36241 gggatgcggg atatgagtgg cgcttttaac aacgatggtc ggggcatatc tcccttaatt
    36301 gcaacctcct gggagagatg caataagctg atgaaacggg agacatggaa cgtaccacac
    36361 caggcccagg gcgtgacatt tgcttctatt tatcggcgta agaaagcgat gctgacgctc
    36421 gggcaggctg cgctggaaga tgcctgggaa tatatggcac cgcgagagtg tgcgttgctt
    36481 atcctcgatg aaaccgcctg cattctcagc cgtaatggcg atccgcaaac cttgcagcaa
    36541 ctaagtacac tgggattcaa tgacggcacg tattgcgccg agggaattat tggtacttgt
    36601 gcgctatcgt tagcggctat ctctggtcag gccgtgaaaa cgatggccga tcaacatttc
    36661 aaacaggcac tctggaactg ggccttttgt gcaacgccgt tgtttgacag caagggccga
    36721 ttgacgggaa caatagcgct ggcgtgtccg gttgagcaaa ctaccgcagc tgatttgccg
    36781 ttgacgttgg caatcgcacg cgaggtcgga aatttactgc tgacggacag tttgctcgct
    36841 gaaactaacc gtcatttaaa tcaacttaat gccctgttag aaagtatgga tgatggtgtg
    36901 attagctggg acgagcaggg tcatttgcaa tttatcaatg cccaggcggc gcgggtcttg
    36961 cgccttgacg cgacggcaag tcagggaagg gcgatcactg aactcttaac gttacccgcc
    37021 gtattgcaac aagcaataaa acaggcacat ccgctcaaac acgtagaagc aacctttgaa
    37081 agtcagcatc agtttattga tgcggtgata acccttaaac cgataataga aacgcaggga
    37141 accagcttta ttttgttgct ccatcctgtg gaacagatgc ggcagttaat gaccagtcaa
    37201 ttaggaaaag tcagccatac tttcgctcat atgccacaag aggatccgca aacccgccgc
    37261 ttgattcatt ttggtcgcca ggcggcgcgc agtagctttc ctgtcctgct ttgtggagag
    37321 gagggcgtgg gcaaggcact gctaagtcag gcaattcata atgaaagcga gcgtgcagcg
    37381 gggccttata ttgccgtcaa ttgtgagtta tatggtgatg cggcgctggc ggaagaattt
    37441 attggtggcg atcgcacgga taatgaaaat ggccgtctga gtcggctgga actggcgcac
    37501 ggcggcacgc tgtttcttga aaagattgaa tatctggcgg tggagttaca gtctgctttg
    37561 cttcaggtta tcaagcaggg tgttatcacg cgactggatg cgcggcgttt aatacccatt
    37621 gatgtcaaag tgattgccac aacgaccgcg gacctcgcaa tgctggtgga acaaaatcgc
    37681 tttagtcgcc agctgtatta cgcgctgcat gcatttgaaa ttaccatccc gcctctgcgt
    37741 atgcggcgtg gcagcattcc ggcgctggtg aataacaaat tacacagtct tgaaaaacgc
    37801 ttctctacgc ggctgaaaat tgatgacgat gccctcgctc gcctggtttc ctgtgcatgg
    37861 cctggcaacg attttgaact ttacagcgtc atcgagaatc ttgctctgag tagtgataac
    37921 gggcgcattc gcgtcagtga tttacctgaa catctgttta ccgagcaggc gacagatgat
    37981 gtcagcgcca cccgcctttc caccagtctg tcatttgcgg aagttgaaaa agaggcaatt
    38041 attaacgcag cccaggtcac aggcggtcgc attcaggaaa tgtcggcttt acttggaatc
    38101 ggccgcacta cgctgtggcg gaaaatgaag caacatggca ttgatgcagg gcagtttaag
    38161 cgccgggtat gaaagacaga aacgatttat gatacatcag agtgatctgt atttcattcc
    38221 ggcgcacgct aacaattttc agcatcgttt aagggcttgt ctatccccca cttaaaagct
    38281 gaagcgatat cctccgttga cttgcttctg atcaaagagg ttaccctgcg tgtaatccgc
    38341 ttcgagatag aatgtgtgct gtttgttata ctgtgcactg actcccacgc cgttattcca
    38401 gccattacct ttgaaactgt acttctcccg ggagttgttc aacagatatt cggtatcccc
    38461 tgaaaactcg cggatagcgc cagtcttcac atagacattc agctggctgt tgcctgcggt
    38521 gatgtcatat cccagtatca tgctggcacg ccccagcagc gattcgtagt gattcagatg
    38581 tatattgagg ccattactcg ccttcatagc catctcattc tggtggctgt atgtgagctg
    38641 ggtttgcggc tctatataga acccataacc agtaggggac aggttgaacc tctgcccggc
    38701 ttccagggag atactcattc cattcgcagt gccgttggcg ttaacgccgt tgttctgact
    38761 gtccagtacg tggaaactat ttttctggcg cgatgctttt ataacgagat cgctgtaaaa
    38821 accgttttgt gccatgtaac tggcgtacat tcccatgtag tctgaacgtg cggtaccgtt
    38881 gcctccgcta tagtccggcg atgcatgtgt tgagccaata tacagaccga catacaacgg
    38941 cattacatca gagagacgtt tatccccacc aaactggata ccgctgtaac ccatgtcaaa
    39001 gccgctcagt ttgccactgg cgaaggagtc cagacttccc ccataactgc gcagccagat
    39061 atttccgtct ttactctgat ttcgcaggtc acccatccgt tgcatcagcg tacggttttc
    39121 aacatagttc aataagtaac cgacattgag ataattgccg ccagcatctg cggtcgtggt
    39181 gggtttaggt gtgggagtgg ggttaggcgt aggatcgggg ttgactatgg ggggctgagc
    39241 gggagctggt gtgggctcag gattaggagt cggctccgga attgtcccgg aagcataaag
    39301 ctcccagtta gtgccatttt tacgcacatc atacagatat cctcccaact caacctgaga
    39361 agacgcgctg aacgaggccg cgccgtcagt ggtttttacc actgtcagaa cttcatttcc
    39421 cgttgtggcc tcgctgccct ggttacggat agccaatacg tgattaccag cactactccc
    39481 gctgatattc aataaatccc ctttattatt aacgccatta ccctcgccaa caacatcagc
    39541 acgcataata aaggtactgt taccgctcag gttctctacg tttaatgtgg taaatgtgcc
    39601 ggcagttgac gtgtggctgg caaaatcgac agttgaatgg ctcagcgcca gcgtgtcgag
    39661 attagaatta cttgttacgt tccagacgct gttattcatt gcaacgtcca gtttcccgcc
    39721 attgacatta tcgctgaggg aagaacctgt ccaaaacgac ccagggtgca tatccagatt
    39781 gattagccca cctttggaga gaacgctacc gttgataagc atacgacccg aagcgttgat
    39841 gcggctggcg gcataaccat cgtcatgttg cgttgcgatg gccatctggt cgggtgtact
    39901 catatcaatg accagatcgc tcgtgagatc gatctggctg ttggtcatgg cataaatccc
    39961 cctggctcct gccgcgccgg tgatagccaa actgtctccg gttattctac cgccgctgag
    40021 cgcccacaaa cccagcgcca gactgccatt acgatcaacc gtaatatcgg tattttgcat
    40081 gttgataaca gccgttgccg tctgggctga ggcaccataa gaaccgctgg aaaagatgct
    40141 gtttcgttgc gctgccgtgc cagaaaaatt gattgtcgcg tctgaaccac tggttacgag
    40201 gccaccgccc tgcgcggaag aaatatggct atcagcaccg atagtggttg taccaccacg
    40261 aacttcgacg ccatttgcca cggctccagt tacatcaaca gtgagtgcat tcgcgctcac
    40321 ctggccaaag ctccagaggc cgtgtgcatt atcgccattg gttttaatgg tactgtttgt
    40381 tccgagatcg acaacagagt ttttctgtac gtttatcccc atggcgctgt agccctgaac
    40441 atcgattgtc aggtctgtcg ccgtaaaacg cgcagcacca ttggcgttat tgccgttgag
    40501 accaccgata taaacacctg tacttccatc ggtcttgatt ttacttccgc ttccaagatc
    40561 gacactggtg ccatagtcat tgatggttag gcctataccg ttcgagtttt caatggtgaa
    40621 ttgagtggct gttagtgttg agctatgtcc aataatgatg ccgtcatcgt tactcttaat
    40681 ggtgctgcct gtgcctaagt cagcatgggt atagtcacca attaaattaa tgccgatggc
    40741 agaggtttga ccaacaacat ctactgtgag tcggttagct gtcagggagc tgttcttgcc
    40801 ctgaataatg attccctttg catatccaga ggcgtcgttg acattgacgg tgacatcatt
    40861 acccaggttg atattgccag gcgtgtttcc tgctggggtc attacaccat agaggtttcc
    40921 tgatggatcg gcggtatctc ctgtaattct gtcaccatca ctaatagtaa tagtggtgcc
    40981 attttgactt cctaaatcag cagctatcag caggggggaa aagataattg cattcaaacc
    41041 aatagcgcca tattttatca gttgaatgga gggcgaacgg atttttaatt cagcgagacg
    41101 atcggctttg gtattcccat tgtgttgttt gatgcccata ccactcctat atagtaccca
    41161 ggctatgggt ctatggattt tgtcattttg tttaagtcaa tgaataatat cctcttatca
    41221 tcatgatatt tgtatggtgc aatcatgatg agctgctctg agaataattc tctattaccg
    41281 ggcaaaatca ggaacacagg aattaatata tatttaacat cccccttaca aaggtgagtt
    41341 tagatcgagt tattccctaa gttgtcgtta cttactccaa ttcgttacta tgaatatgac
    41401 aaaaacatta caacaaacag cggttaaata atttggtgaa tatttttggt aataatccta
    41461 attagttatg ggattttcgc ttgatgtatc tgcataacaa aaacaatatg agccattttt
    41521 gcattactaa taagaaaaag caaaaaacat cgatattcat ttttgatcaa tggattttct
    41581 ggcatatcgt tattatcgtg atactggtta tcattttcat cttgccaggt tcgcacgtaa
    41641 tcacgttatt cggggatttt atctgcggga tattaccctt caggtaagtc cttttctcgc
    41701 cagcatattg gagcgtgatc aatattgatc agctgtaaaa aggtgtctga tgtgcagtgg
    41761 aacacaaact tacgttatct cattgctaat gagatatccc tcatctgaac acttatgtct
    41821 ttaattattc tcgtggttca ctataggcaa taagcacaaa agtgtaggat gttataagaa
    41881 tggttaggac tcggtgaaat gaaaaatcca cgcaattgcg tggattttat atacttttgc
    41941 gctcttcatg agatttagcg aaacctcatg agacaacaaa ttaattagac gttgaacagg
    42001 aagttcatta catcgccatc tttaacgatg taatctttgc cttctgcacg cattttgcct
    42061 gcttctttcg cgccttgttc acctttgtaa gtgatgaaat cttcaaacga gatggtttgt
    42121 gcacggataa agcctttttc aaaatcagta tggattttgc cagcggcctg cggcgcggtt
    42181 gcaccaaccg gaatggtcca tgcacgtact tctttcaccc cagcggtgaa gtaagtttgc
    42241 aggttcagca gcttataacc ggcacggatc acacggttca ggcccggctc ttccagccca
    42301 agctcctgca taaactcgtc acgttcttcg tcgtccagtt cggcaatgtc tgcttcaaca
    42361 gcagcacaaa ccggaaccac aacagaacct tctttcgccg cgatttcacg cacctggtca
    42421 agatacgggt tgttttcaaa accgtcttcg ttgacgttgg cgatgtacat tgttggtttc
    42481 agcgtcagga agctcaggta acgaatagcc gttttctctt cagcgcttaa atccagcgcg
    42541 cgcagcatac ctgcgttttc cagctgcggc aggcattttt ccagtaccgc cagctcggct
    42601 ttcgcgtctt tatcgccacc tttggctttc ttctgtacgc gatgaatcgc acgttcgcag
    42661 gtgtcgaggt ccgccagcgc cagttcggtg ttgataactt caatatcatc agccgggtta
    42721 actttgccgg aaacgtgaat gatgttgtca ttttcaaagc agcgaacaac gtgaccgatc
    42781 gcttcagttt cacggatgtt ggtcaggaac tggttaccca gaccttcgcc tttcgatgcg
    42841 ccttttacca gaccggcgat atcgacaaat tccatggtcg tgggaagcgt acgctgcggt
    42901 tttacgattt cagccagttg atccaggcga gggtcaggca ttggtacgac gcctgtgttc
    42961 ggctcaatgg tgcagaatgg aaagttggcc gcttcaatac cggctttggt cagcgcgttg
    43021 aacagggtag atttcccgac gttgggcaaa ccgacgatac cgcatttgaa tcccatgatt
    43081 taaatcacct taatatctta ataatcaacc tgttattgct aacagattgc agaaatggaa
    43141 ataactttgc ctattataca cggcactcgg caaaaatgcc gcagacaacg acttattgcg
    43201 ctttaaaggc gtgcaatcgg ttcgttgctt tggtcaagcc atctgtaaac cacatttcag
    43261 tacaacgcgc cgcttcgtcg atggcttcat caattaactt ctgttcacta acaggcggtt
    43321 tgcctaatac aaaaccaaca actttatttt tatcgcccgg atgaccgatt ccgatgcgta
    43381 aacggtgaaa gttagggtta ttacccaatt tactgatgat gtctttcagt ccattgtgac
    43441 caccatggcc accgcccaac ttaaatttgg cgacgccagg aggcagatcc agttcgtcgt
    43501 gggccaccag aatttcgtcc ggattaatgc ggaaaaaact ggccatcgcc gcaacggctt
    43561 tgccgctgag attcataaat gtagtcggga ctaacaggcg gacatcttcg cctccaaggg
    43621 tgactcgcga agtataacca aagaatttag cctcttcgcg cagcggagcg cgcaatcgct
    43681 ctgccagtaa gtcaacgaac caggcaccag cattatgtcg tgttgcggcg tattcagcgc
    43741 cggggttcgc caggccgaca atcaatttaa tcgtcacgtt tttttgtcct gagtgtgtac
    43801 ataactggcg cgtagtttac tggttgcggc cccgcttgac aaaaaactgc gtatcaaatg
    43861 cagataacgc aataattgcc tgagtggact attagaaagt caaggtgttc aggcgtttat
    43921 ttgtaaagtt ttgttgaaat aagggttgta attgtgatca cgcccgcaca taacccacag
    43981 ggtgttgtct atactttaca cataaggaag aggggtattc cctgttacaa cccagaaagt
    44041 tccggaggtg acatatgaaa cgcaaaaacg cttcgttact cggtaacgtg ctcatggggt
    44101 tgggtctggt ggtaatggtg gtcggcgtgg ggtattcaat cctcaaccag ttaccacagt
    44161 ttaatatgcc ccagtatttc gcacatggtg cagtgctaag tattttcgtc ggtgccattc
    44221 tctggctggc gggtgcccgt gttggcgggc atgaacaggt gtgcgaccgt tactggtggg
    44281 ttcgccacta tgacaaacgt tgccgccgta gcgataatcg ccgtcatagc taacaaataa
    44341 tgcagtttgc tgaccagtca gtttgcgctg actggtcaat ctcgtactta taaatccgcc
    44401 atcgccgcgc gacgattcgg gaagaatgcg aggcgtcccg ggatcggttg aatgcctgcg
    44461 cgagccatag tgcgcagtgg ctggaattcc aggttgcaca cgcgcagttc acatccttcg
    44521 ggcagacgct ttacaaaacg ctggaacgca tcaagaccac cagcatcaag taccggaacg
    44581 gcatcccact tcagaatcac aatccgtttg ccttcaagac gtgactccag gtccgtgaac
    44641 aagccttcag cggcagcaaa aaacagcgga ccaataacgc gcagaaccag aacatcgtct
    44701 ggaacgtcta cgactaccgg tgccaggcga gtcatacgtg cgatacgacg cataaacagt
    44761 agcgatgcca gcacgatccc cacgctgatg gcaataacca tatcaaacag cacagtcagc
    44821 gacatgcaca gcagcatgac gatgatgtca tctttcggcg catgacgcag caagtcgacc
    44881 actttgtgcg cttcactcat gttccacgcc accatcaaca gcagggcagc catggcggaa
    44941 agcggcagcc aggagagcag cggtgccagt accagcaggg caagaataac cagaatagag
    45001 tggatcaccg ccgagatagg ggaagttgcc ccggcacgga cgttagcggc agaacgcgcg
    45061 atggcagctg tagcggtaat accaccaaag aacggggcga tgatattccc cagtccctgt
    45121 ccaaccagtt cgctgttcgc tttgtgtttc gttccggtca taccatccag taccacggcg
    45181 cagagcagag attcgattgc gccgagcatt gccattgaga atgccgcagg cagcagtgtg
    45241 cgaatagaat cccaggtgag ggtaaattct gaattgggca ggtcccacgg tagtaccagt
    45301 tgcggcagca gttgcggaat accgttaccc tgagaaccat ctgccagaac gtagtggaat
    45361 tgcgatccga tggtagcaac atgcccgccg agcaggttaa caatccccat caccgcgcaa
    45421 ccagccagca aggccggaag gtgaccaggc aaacgaatgc ccagacgcgg ccagaaaacc
    45481 agaatgccaa gcgtcacaat gccaatggca gcatcaccca cattaatggt cggcaacgcc
    45541 ataaataatg cgccgacttt ttgtagataa tgctccggga catgggccat ttgcagaccg
    45601 agaaaatctt taatctgcat ggtaccgatg gtgatcccga tacccgaggt gaaacctaag
    45661 gtcacggaaa ccggaatata ttcaatcaag cgtccaaaac gtgccagccc catcaggatc
    45721 aaaaagacac ctgacatgag tgtcgcgacc agcagtcctg ccagtccaaa ctgttgcgaa
    45781 acgggataga gaattaccac aaatgccgca gtcggaccgg aaacgctaaa gcgtgaccca
    45841 cccgtcagag caatgacaat ccccgcaaca gctgcggtat ataaaccgta ctggggtgcc
    45901 acaccactac caatagccaa cgccattgcc agcgggatag caataatccc gacggttatc
    45961 ccggcaatca ggtcacgggt aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg
    46021 atcagagcgc ggaaaggcat cacatgtgag gaaaatattt tgttcacaat aatgtttcat
    46081 ccgtgagcgc atcatctgtc aactaaatgg caggtgaagg aggcataggt catacaaatg
    46141 gatattacag acaaaaaaac ccgccgcagc gggtctttga gccgggttcg attagtgttc
    46201 gaacatggca gagatcgatt cttcgttgct gatacgacga atcgcttcgg ccagcatacc
    46261 tgacagggtc agagtacgca cgttcggcag tgatttgatt tcatcgctca gcggaatggt
    46321 atcgcagaca acgacttcat caattacaga gttacgcagg ttgttcgccg cgttgccaga
    46381 gaagatcggg tgagtcgcgt acgcaaatac acgtttagca ccacgttctt tcagagcttc
    46441 agcagcttta cacagcgtac cgccagtatc gatcatatca tcgaccagta cgcagtcacg
    46501 acctgcaacg tcaccgatga tatgcatcac ctgggaaacg ttcgcacgcg gacgacgttt
    46561 gtcgatgatt gccatatcgg tatcgttcag cagcttagcg atagcgcggg cacgcacaac
    46621 gccgccgatg tccggagaaa ccacaatagg gttatccaga ttcagctgca gcatgtcttc
    46681 cagcaggatc gggctaccaa atacgttatc aaccggaacg tcgaagaaac cctgaatctg
    46741 ttcagcgtgc agatccactg tcagcacacg gtcaacaccg acgctggaga ggaagtctgc
    46801 aaccactttc gcagtgattg gtacacgagc ggaacggacg cgacggtcct ggcgcgcata
    46861 gccaaagtag gggataacag cggtgatacg acctgcggaa gcacgacgca gggcatcaac
    46921 cataacgact aattccatca ggttgtcgtt agtaggggca caagtggact ggatgatgaa
    46981 aatatcacca ccgcgtacat tttcgttaat ttgtacgctg acttcgccgt cgctaaagcg
    47041 acctacagcg gcgtcgccga gtgaagtgta caggcggttg gcaatacgtt gtgctagttc
    47101 cggggtggcg ttaccagcaa aaagcttcat atcaggcacg agaagaacct caggcatgcg
    47161 tccattggtg gaaagaatct gccgaaaact gtgcgggcca ggcatgattc tttccaggcg
    47221 gtgtattaaa gagcgcgatg caacgtctgg aacaaggtga cgttgtcacc gaaactcagc
    47281 ttgcccggct taaagcatgg ctctgtgcaa tggggaaaga ttaacgcctt tcgccacaaa
    47341 gccattgagc cattccgggg cttgctctag cacctggcgg gcttcagact ctgtatcaaa
    47401 ttcagcaaag acacaggccc ctgtcccagt caggcgcgac ggggcgtatt ctaacagcca
    47461 ggaaagcacc gcatcaacct cgcgaaaacg ttttcttgcg ataacctcgc aatcattgct
    47521 gaattcacat tttagcaacg tttctattga cctttttggc gtattgcgcg ggagttcagg
    47581 atctttaaaa atcactggtg tcggaatact tacaccaggg tgcgccacca gataccactt
    47641 ctctggcgga tccaccggcg ttagtatttc accaacgcct tcggcaaacg cggcatgccc
    47701 ccgaacaaag acaggaacat ctgcgcccag cgtcagcccc atttccgcca gctcatccat
    47761 gcttagcccg cattgccaga gatgatttaa tgccaccagg accgtcgcgg cattggatga
    47821 accaccgccg agaccgccgc ccatcggcaa acgcttgtca atgctgatat ccgcaccgct
    47881 tcccgtcgga agacgcccgc tgtctgccgc agttttcatc aacaatcgtg ctgcgcgaac
    47941 gatcaggtta tcttcatgtt ccacgccttc aacgggcgtt aacagacgaa tatccccatc
    48001 gtcacgaagc tcaatgctga tggtgtcgcc gtaatcaaga aactgaaaca gcgtttgcag
    48061 cgtgtggtaa ccatccgcac gctgaccggt aatgtataaa aacagattaa gttttgccgg
    48121 agagggccac tgtgtccgca ttatttcact atccagttat ccatttttaa cttgatgcgt
    48181 tgaccaccgt cggtgagttc catattggct ggcatcgcag gttgagtttt tgtgtcataa
    48241 ccaccgtaaa caactttcca gtttttgcca ttctggctgt aggtgatttc gctcaggcgg
    48301 tactggtcgt ccagtttgta gtcggttgca tcacccggta aacctaaaat ccactggcgc
    48361 aagctgttga gcggaattgg cattccggtc aatttgccaa tcatctcttc ggcgtcatcg
    48421 gcggtataac gctggccttt attgtcgact aactgcacgt tacccggttg agcattcagc
    48481 tccagttccg tgctgcctaa tgggttagtc agcagcagac ggtagcgatc ctggccagtt
    48541 tgctgccaga agaaacgggc gtacactttt tgttggtcag aaatataagc gaacgcgccg
    48601 cgagtttgat actgattaag attgcgcacg tcttgctgat gctgacgcca ttgtggtgaa
    48661 tccgggcttt tgccaggacc tttgggtgtg gtaacggaac aggcagtgag cacaagagca
    48721 gccagcggta gcaggcggat aagacgaaaa tcgggcaggg gcatagtgat gacaagtcct
    48781 tgagatacgt tgcagttata acccttaatg ctagcgttac cgtccgctat cgtctatgtt
    48841 caagttgtct taattgccag aatctaacgg ctttcggcaa ttactccaaa agggggcgct
    48901 ctcttttatt gatcttacgc atcctgtatg atgcaagcag actaacccta tcaacgttgg
    48961 tattatttcc cgcagacatg acccttttag cactcggtat caaccataaa acggcacctg
    49021 tatcgctgcg agaacgtgta tcgttttcgc cggataagct cgatcaggcg cttgacagcc
    49081 tgcttgcgca gccgatggtg cagggcggcg tggtgctgtc gacgtgcaat cgcacggaac
    49141 tttatcttag cgttgaagag caggataacc tgcaagaggc gttaatccgc tggctatgcg
    49201 attatcataa tcttaatgaa gaagatctgc gtaaaagcct ctactggcat caggataacg
    49261 acgcggttag ccatttaatg cgtgttgcca gcggcctgga ttcactggtt ctgggggagc
    49321 cgcagatcct cggtcaggta aaaaaagcct ttgctgattc gcaaaaaggt catatgaagg
    49381 ccagcgaact ggaacgcatg ttccagaaat ctttctctgt agcgaaacgc gttcgcactg
    49441 aaacagatat cggtgccagt gctgtgtctg tcgcttttgc ggcttgtacg ctggcgcggc
    49501 agatctttga atcgctctct acggtgacgg tactgctggt gggtgcgggc gaaaccatcg
    49561 agctggtggc gcgtcatctg cgcgaacata aagtacagaa gatgattatc gccaaccgca
    49621 ctcgcgaacg tgcccaaatt ctggcagatg aagtcggcgc ggaagttatt gccctgagtg
    49681 atatcgacga acgtctgcgc gaagccgata tcatcatcag ttccaccgcc agcccgttac
    49741 cgattatcgg gaaaggcatg gtggagcgcg cattaaaaag ccgtcgcaac caaccgatgc
    49801 tgctggtgga tattgccgtt ccgcgcgatg ttgagccgga agttggcaaa ctggcgaatg
    49861 cttatcttta tagcgtggac gatctgcaaa gcatcatttc gcacaacctg gcgcagcgta
    49921 aagccgcagc ggttgaggcg gaaactattg tcgctcagga aaccagcgaa tttatggcgt
    49981 ggctgcgtgc acaaagcgcc agcgaaacca ttcgcgagta tcgcagccag gcagagcaag
    50041 ttcgcgatga gttaaccgcc aaagcgttag cggcccttga gcagggcggc gacgcacaag
    50101 ccattatgca ggatctggca tggaaactga ctaaccgctt gatccatgcg ccaacgaaat
    50161 cacttcaaca ggccgcccgt gacggggata acgaacgcct gaatattctg cgcgacagcc
    50221 tcgggctgga gtagcagtac atcattttct ttttttacag ggtgcattta cgcctatgaa
    50281 gccttctatc gttgccaaac tggaagccct gcatgaacgc catgaagaag ttcaggcgtt
    50341 gctgggtgac gcgcaaacta tcgccgacca ggaacgtttt cgcgcattat cacgcgaata
    50401 tgcgcagtta agtgatgttt cgcgctgttt taccgactgg caacaggttc aggaagatat
    50461 cgaaaccgca cagatgatgc tcgacgatcc tgaaatgcgt gagatggcgc aggatgaact
    50521 gcgcgaagct aaagaaaaaa gcgagcaact ggaacagcaa ttacaggttc tgttactacc
    50581 aaaagatcct gatgacgaac gtaacgcctt cctcgaagtc cgtgccggaa ccggcggcga
    50641 cgaagcggcg ctcttcgctg gcgatctgtt ccgtatgtac agccgttacg ccgaagcccg
    50701 ccgctggcgt gtagaaatca tgagcgccag cgagggtgaa catggtggtt ataaagagat
    50761 catcgccaaa attagcggtg atgttgtata tggtcgtctg aaatttgaat ccggcggtca
    50821 tcgcgtgcag cgtgttcctg ctacggaatc gcagggtcgt attcatactt ctgcttgtac
    50881 cgttgcggta atgccagaac tgcctgacgc agaactgccg gacatcaacc cagcagattt
    50941 gcgcattgat actttccgtt cgtcaggggc aggtggtcag cacgttaaca ccaccgattc
    51001 ggcaattcgt attacccact tgccgaccgg gattgttgtt gaatgtcagg acgaacgttc
    51061 acagcataaa aacaaagcta aagcactttc tgtgcttggt gctcgcatcc acgctgcaga
    51121 aatggcaaaa cggcaacagg ccgaagcgtc tacccgtcgt aacctgctgg ggagtggcga
    51181 tcgcagcgac cgtaaccgta cttacaattt cccgcagggg cgcgttaccg atcaccgcat
    51241 caacctgacg ctctaccgtc tggatgaagt gatggaaggt aagctggata tgctgattga
    51301 accgattatc caggaacatc aggccgacca actggcggcg ttgtccgagc aggaataatg
    51361 gaatatcaat actggttacg tgaagcaata agccaacttc aggcgagcga aagcccgcgg
    51421 cgtgatgctg aaatcctgct ggagcatgtt accggcagag ggcgtacttt tattctcgcc
    51481 tttggtgaaa cgcagctgac tgacgaacaa tgtcagcaac ttgatgcgct actgacacgt
    51541 cgtcgcgatg gtgaacccat tgctcattta accggggtgc gagaattctg gtcgttgccg
    51601 ttatttgttt cgccagcgac cttaattccg cgcccggata cggagtgtct ggtggagcag
    51661 gcactggcgc ggttgcctga acaaccttgc cgtattctcg atctcgggac gggtaccggg
    51721 gcgattgcgc tggcgctggc tagcgagcgc ccggactgcg aaattaccgc tgtagatcgt
    51781 atgcccgatg ctgtctctct ggcgcaacgt aatgcccaga atctggcgat caaaaacatc
    51841 catattctgc aaagcgactg gtttagcgcg ctagccgggc agcagtttgc gatgattgtc
    51901 agtaatccgc cgtatattga cgagcaggac ccacatcttc aacaaggcga tgttcgcttt
    51961 gagccgctca ctgcgctggt tgcggcagac agtggaatgg cagacatcgt gcatatcatc
    52021 gaacagtcgc gtaacgcgct ggtatccggc ggctttctgc ttctggaaca tggttggcag
    52081 cagggcgaag cggtgcgaca ggcatttatc ctcgcggggt atcatgacgt cgaaacctgt
    52141 cgggactatg gtgataacga gcgcgtaacg ctcggccgct attatcaatg acaagttttt
    52201 ctacactgct tagcgttcat ctcattagca ttgcgctttc tgtagggcta ttaaccttac
    52261 gtttctggct acgttatcag aagcatccac aggcatttgc gcgctggacg cgcattgtgc
    52321 cgccggttgt cgatacggtg ttattgttaa gcggcattgc gttgatggct aaagcgcaca
    52381 tcctgccatt ttccgggcag gcacagtggc tgactgaaaa gctgtttgga gttatcattt
    52441 atatcgtttt gggttttatt gcattcgatt atcgtcgtat gcacagtcag caggcgcgca
    52501 ttattgcctt cccgctggcg ttggtggtgc tgtacatcat cattaaactc gccaccacaa
    52561 aagtaccgtt actggggtaa gtcatgagat cgttagctga tttcgaattt aataaagcgc
    52621 cattgtgcga aggcatgatc ctggcttgcg aagcaatccg ccgcgatttt ccctcgcaag
    52681 atgtttacga cgaactggag cgtctcgtta gtctggcgaa ggaagaaatc agccagcttc
    52741 tgcctttaga agagcagttg gaaaaactga tcgcgctgtt ttacggcgac tggggattta
    52801 aagcctcacg cggcgtttat cgcctttccg atgcattatg gctggaccag gtgttaaaga
    52861 atcgacaggg cagtgcggta tcattaggtg ccgttttatt atgggtcgcg aatcgtctcg
    52921 atttgccgct attgccggtg attttcccga cgcagctgat attgcgcatt gaatgtccgg
    52981 atggcgaaat ttggctgatt aatcctttta acggtgaatc gttaagcgaa catatgctgg
    53041 atgtatggtt aaagggaaat atcagcccgt cggcggaact gttttatgaa gaccttgatg
    53101 aagctgataa cattgaggta atccgcaaat tgctggatac actcaaagct tcgttgatgg
    53161 aagaaaatca gatggagctg gcgttacgca ccagcgaagc tttattacaa ttcaacccgg
    53221 aagatcccta tgaaattcgc gatcgcgggt tgatttatgc gcaactggat tgcgaacacg
    53281 ttgcgttgaa cgatttaagt tatttcgttg aacagtgtcc ggaagacccg atcagcgaaa
    53341 tgatccgtgc gcaaataaat aacatcgcgc ataaacatat tgtgctgcat taattaatcg
    53401 acattttact caagattaag gcgatcctat gaaacaaaaa gtggttagca ttggcgacat
    53461 caacgtagca aatgacctgc cgttcgtact gtttggcggt atgaacgtgc tagaatctcg
    53521 cgatctggcg atgcgcattt gcgagcacta cgtaaccgtg acccagaaac tgggtatccc
    53581 ttacgtgttc aaagcctctt ttgacaaagc caaccgctcc tccatccact cttatcgtgg
    53641 accgggcctg gaagaaggga tgaaaatctt ccaggagttg aagcagactt ttggcgtgaa
    53701 aattatcacc gacgttcacg aaccaagcca ggctcagccc gttgctgatg ttgtggatgt
    53761 gattcagttg ccggcgtttc ttgctcgcca gactgacctg gttgaagcca tggcgaaaac
    53821 cggtgcggta attaacgtca agaaaccgca gtttgtcagc ccgggacaga tgggtaatat
    53881 cgttgataaa ttcaaagaag gcggcaacga aaaagtgatt ctttgcgatc gcggtgctaa
    53941 cttcggctat gacaacctgg ttgttgatat gctgggcttc agcatcatga agaaagtgtc
    54001 tggtaattca ccggtgattt tcgacgtgac ccacgcactg caatgccgcg atccgtttgg
    54061 cgcagcttcc ggcggtcgtc gtgctcaggt ggctgagctg gcacgtgccg gtatggcggt
    54121 aggtctggcg gggctgttta ttgaagcgca tccggatccg gaacatgcga aatgtgatgg
    54181 tccatccgcg ctgccgctgg ctaaactgga accgttcctc aagcagatga aagcgattga
    54241 tgatctggtg aaaggtttcg aagaactgga taccagcaag taatcttttt tgcttgaaaa
    54301 ctaaagcctt agcgttctgc gttaaggctt ttttgtgcac agaaaacccc cagctaggct
    54361 gggggttccg gaaagctttc agcttt
//
LOCUS       DAOTRK010000013        48750 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.013, whole genome shotgun sequence.
ACCESSION   DAOTRK010000013 DAOTRK010000000
VERSION     DAOTRK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.013"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..288)
                     /locus_tag="QIE41_000862"
                     /pseudo
     CDS             complement(1..288)
                     /locus_tag="QIE41_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            563..1357
                     /locus_tag="QIE41_000863"
     CDS             563..1357
                     /locus_tag="QIE41_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDS3529781.1"
                     /translation="MTLPTTIYSFPAYSPRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRHTVEIHKLDLSDWQAFNKLATRCNTYDGITMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQGRDIRCDHMHLTPANHPELWTQENGEWIYLRN"
     gene            complement(1428..1877)
                     /gene="rplI"
                     /locus_tag="QIE41_000864"
     CDS             complement(1428..1877)
                     /gene="rplI"
                     /locus_tag="QIE41_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDS3529782.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1919..2146)
                     /gene="rpsR"
                     /locus_tag="QIE41_000865"
     CDS             complement(1919..2146)
                     /gene="rpsR"
                     /locus_tag="QIE41_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDS3529783.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(2151..2465)
                     /gene="priB"
                     /locus_tag="QIE41_000866"
     CDS             complement(2151..2465)
                     /gene="priB"
                     /locus_tag="QIE41_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDS3529784.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(2472..2867)
                     /gene="rpsF"
                     /locus_tag="QIE41_000867"
     CDS             complement(2472..2867)
                     /gene="rpsF"
                     /locus_tag="QIE41_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDS3529785.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            3194..3469
                     /locus_tag="QIE41_000868"
     CDS             3194..3469
                     /locus_tag="QIE41_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDS3529786.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHPILIREPGSNGNMHASADIYR"
     gene            complement(3598..4284)
                     /gene="ulaF"
                     /locus_tag="QIE41_000869"
     CDS             complement(3598..4284)
                     /gene="ulaF"
                     /locus_tag="QIE41_000869"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDS3529787.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4284..5138)
                     /gene="ulaE"
                     /locus_tag="QIE41_000870"
     CDS             complement(4284..5138)
                     /gene="ulaE"
                     /locus_tag="QIE41_000870"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDS3529788.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5148..5798)
                     /gene="ulaD"
                     /locus_tag="QIE41_000871"
     CDS             complement(5148..5798)
                     /gene="ulaD"
                     /locus_tag="QIE41_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDS3529789.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5812..6276)
                     /gene="ulaC"
                     /locus_tag="QIE41_000872"
     CDS             complement(5812..6276)
                     /gene="ulaC"
                     /locus_tag="QIE41_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDS3529790.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6286..6591)
                     /gene="ulaB"
                     /locus_tag="QIE41_000873"
     CDS             complement(6286..6591)
                     /gene="ulaB"
                     /locus_tag="QIE41_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDS3529791.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6607..8004)
                     /gene="ulaA"
                     /locus_tag="QIE41_000874"
     CDS             complement(6607..8004)
                     /gene="ulaA"
                     /locus_tag="QIE41_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDS3529792.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8359..9423
                     /gene="ulaG"
                     /locus_tag="QIE41_000875"
     CDS             8359..9423
                     /gene="ulaG"
                     /locus_tag="QIE41_000875"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDS3529793.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9531..10286
                     /gene="ulaR"
                     /locus_tag="QIE41_000876"
     CDS             9531..10286
                     /gene="ulaR"
                     /locus_tag="QIE41_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDS3529794.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10283..11032)
                     /gene="yjfP"
                     /locus_tag="QIE41_000877"
     CDS             complement(10283..11032)
                     /gene="yjfP"
                     /locus_tag="QIE41_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDS3529795.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFRGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLADRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11214..11543
                     /gene="bsmA"
                     /locus_tag="QIE41_000878"
     CDS             11214..11543
                     /gene="bsmA"
                     /locus_tag="QIE41_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDS3529796.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11692..11967
                     /gene="yjfN"
                     /locus_tag="QIE41_000879"
     CDS             11692..11967
                     /gene="yjfN"
                     /locus_tag="QIE41_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDS3529797.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12084..13709)
                     /locus_tag="QIE41_000880"
     CDS             complement(12084..13709)
                     /locus_tag="QIE41_000880"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDS3529798.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIEQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFMEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLNTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(13793..14956)
                     /locus_tag="QIE41_000881"
     CDS             complement(13793..14956)
                     /locus_tag="QIE41_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDS3529799.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDTDQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKLQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14959..15597)
                     /locus_tag="QIE41_000882"
     CDS             complement(14959..15597)
                     /locus_tag="QIE41_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDS3529800.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15607..16005)
                     /locus_tag="QIE41_000883"
     CDS             complement(15607..16005)
                     /locus_tag="QIE41_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDS3529801.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(16023..16682)
                     /locus_tag="QIE41_000884"
     CDS             complement(16023..16682)
                     /locus_tag="QIE41_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDS3529802.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGENIDDIDDIKLFVYE
                     ESYGISKESHWRETINAKAMGAMTLNWQEKRWLRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16733..17431)
                     /locus_tag="QIE41_000885"
     CDS             complement(16733..17431)
                     /locus_tag="QIE41_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDS3529803.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALKALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17450..17851)
                     /locus_tag="QIE41_000886"
     CDS             complement(17450..17851)
                     /locus_tag="QIE41_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDS3529804.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQHEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSL"
     gene            complement(17978..18709)
                     /gene="rlmB"
                     /locus_tag="QIE41_000887"
     CDS             complement(17978..18709)
                     /gene="rlmB"
                     /locus_tag="QIE41_000887"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDS3529805.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(18800..21241)
                     /gene="rnr"
                     /locus_tag="QIE41_000888"
     CDS             complement(18800..21241)
                     /gene="rnr"
                     /locus_tag="QIE41_000888"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDS3529806.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(21280..21705)
                     /gene="nsrR"
                     /locus_tag="QIE41_000889"
     CDS             complement(21280..21705)
                     /gene="nsrR"
                     /locus_tag="QIE41_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDS3529807.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(21910..23208)
                     /gene="purA"
                     /locus_tag="QIE41_000890"
     CDS             complement(21910..23208)
                     /gene="purA"
                     /locus_tag="QIE41_000890"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDS3529808.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(23312..23509)
                     /locus_tag="QIE41_000891"
     CDS             complement(23312..23509)
                     /locus_tag="QIE41_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDS3529809.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(23591..24595)
                     /gene="hflC"
                     /locus_tag="QIE41_000892"
     CDS             complement(23591..24595)
                     /gene="hflC"
                     /locus_tag="QIE41_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDS3529810.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(24598..25857)
                     /gene="hflK"
                     /locus_tag="QIE41_000893"
     CDS             complement(24598..25857)
                     /gene="hflK"
                     /locus_tag="QIE41_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDS3529811.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(25943..27223)
                     /gene="hflX"
                     /locus_tag="QIE41_000894"
     CDS             complement(25943..27223)
                     /gene="hflX"
                     /locus_tag="QIE41_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDS3529812.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(27300..27608)
                     /gene="hfq"
                     /locus_tag="QIE41_000895"
     CDS             complement(27300..27608)
                     /gene="hfq"
                     /locus_tag="QIE41_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDS3529813.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(27694..28644)
                     /gene="miaA"
                     /locus_tag="QIE41_000896"
     CDS             complement(27694..28644)
                     /gene="miaA"
                     /locus_tag="QIE41_000896"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDS3529814.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(28637..30484)
                     /gene="mutL"
                     /locus_tag="QIE41_000897"
     CDS             complement(28637..30484)
                     /gene="mutL"
                     /locus_tag="QIE41_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDS3529815.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RGGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(30494..31831)
                     /gene="amiB"
                     /locus_tag="QIE41_000898"
     CDS             complement(30494..31831)
                     /gene="amiB"
                     /locus_tag="QIE41_000898"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDS3529816.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGTAAQTASTVTTPDRTLPN"
     gene            complement(31850..32311)
                     /gene="tsaE"
                     /locus_tag="QIE41_000899"
     CDS             complement(31850..32311)
                     /gene="tsaE"
                     /locus_tag="QIE41_000899"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDS3529817.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(32283..33830)
                     /gene="nnr"
                     /locus_tag="QIE41_000900"
     CDS             complement(32283..33830)
                     /gene="nnr"
                     /locus_tag="QIE41_000900"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDS3529818.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRTAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALSREAWLNAGGEIHASNTVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRVVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVLVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            33829..34968
                     /gene="queG"
                     /locus_tag="QIE41_000901"
     CDS             33829..34968
                     /gene="queG"
                     /locus_tag="QIE41_000901"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDS3529819.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(34951..35004)
                     /locus_tag="QIE41_000902"
     CDS             complement(34951..35004)
                     /locus_tag="QIE41_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529820.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(35238..35313)
                     /locus_tag="QIE41_000903"
     tRNA            complement(35238..35313)
                     /locus_tag="QIE41_000903"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35278..35280),aa:Gly,seq:gcc)
     gene            complement(35349..35424)
                     /locus_tag="QIE41_000904"
     tRNA            complement(35349..35424)
                     /locus_tag="QIE41_000904"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35389..35391),aa:Gly,seq:gcc)
     gene            complement(35582..35657)
                     /locus_tag="QIE41_000905"
     tRNA            complement(35582..35657)
                     /locus_tag="QIE41_000905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35622..35624),aa:Gly,seq:gcc)
     gene            complement(35868..36413)
                     /gene="orn"
                     /locus_tag="QIE41_000906"
     CDS             complement(35868..36413)
                     /gene="orn"
                     /locus_tag="QIE41_000906"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDS3529821.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            36508..37560
                     /gene="rsgA"
                     /locus_tag="QIE41_000907"
     CDS             36508..37560
                     /gene="rsgA"
                     /locus_tag="QIE41_000907"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDS3529822.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLLIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNTLLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            37657..38625
                     /gene="psd"
                     /locus_tag="QIE41_000908"
     CDS             37657..38625
                     /gene="psd"
                     /locus_tag="QIE41_000908"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDS3529823.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLSAEEIEAEHDASPLVDDKKDQV"
     gene            38647..41970
                     /gene="mscM"
                     /locus_tag="QIE41_000909"
     CDS             38647..41970
                     /gene="mscM"
                     /locus_tag="QIE41_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDS3529824.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPQLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVLIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(41999..42313)
                     /locus_tag="QIE41_000910"
     CDS             complement(41999..42313)
                     /locus_tag="QIE41_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDS3529825.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(42411..43913)
                     /gene="yjeM"
                     /locus_tag="QIE41_000911"
     CDS             complement(42411..43913)
                     /gene="yjeM"
                     /locus_tag="QIE41_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDS3529826.1"
                     /translation="MPHTIKKVSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRITGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWVVE"
     gene            complement(44132..45109)
                     /gene="epmA"
                     /locus_tag="QIE41_000912"
     CDS             complement(44132..45109)
                     /gene="epmA"
                     /locus_tag="QIE41_000912"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDS3529827.1"
                     /translation="MSETASWQPSASIPNLLKRATIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            45434..47242
                     /gene="frdA"
                     /locus_tag="QIE41_000913"
     CDS             45434..47242
                     /gene="frdA"
                     /locus_tag="QIE41_000913"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDS3529828.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            47235..47969
                     /gene="frdB"
                     /locus_tag="QIE41_000914"
     CDS             47235..47969
                     /gene="frdB"
                     /locus_tag="QIE41_000914"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDS3529829.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            47980..48375
                     /gene="frdC"
                     /locus_tag="QIE41_000915"
     CDS             47980..48375
                     /gene="frdC"
                     /locus_tag="QIE41_000915"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDS3529830.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPESWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            48386..48745
                     /gene="frdD"
                     /locus_tag="QIE41_000916"
     CDS             48386..48745
                     /gene="frdD"
                     /locus_tag="QIE41_000916"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDS3529831.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
BASE COUNT        11884 a        13297 c        12055 g        11514 t
ORIGIN      
        1 caggccaagc gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatata
       61 cacctgcaat gggtcgatat ctggcttaaa aatattgtgt ttcttccctt catccaataa
      121 ggattccatt aactgcaagt gcgcatgatt aatttctaag agacgctgtg attttgcata
      181 atgcacacct ttactttggt tttcactatg cacaattttt aaaaaccagg gattagcaat
      241 ataataaccc caggtaaaat cgatgatttt ttcaatggat ttttgtggct ggttaacctg
      301 ctgattaacg aacgcaccga taccgacagc gtagaataat tacataccgc acaacaagaa
      361 tgtatggtgt aaggcgttgt cagtcatctc cctccctggg tgcatcatta tatccgtgat
      421 gagcgatttt tgatattaat atcttttact atccgctatt ttcaccactt cagaaaacaa
      481 acttattttt attaatatca actctttttc tttgaacatt caactgcgat atttgtaaaa
      541 atattaaaca ggcaacaata aaatgacatt accaacaact atttattcat ttcctgcata
      601 ttcaccccgt ttttcgtcca ccgacaaacc agtaaaatta aaatttcatc aatacgcacg
      661 cgctacatta ctttcaaatc gtggaagaga ccataattgc gatggcagac atactgttga
      721 gatccacaaa ctagatctct cagactggca agcattcaat aagttagcga cgaggtgtaa
      781 tacttacgat ggaataacaa tgaatggtga taattctttt ggatggaacc atgaagctac
      841 attagataat attcacgccc agaaatacaa taaagcttac gcgggtgcga ggttaaccgc
      901 agaactgaaa tatttattgc aagatgtcga atccttcgaa cccaacagta aatacacaat
      961 acacgaggtt gttttgggtc caggttatgg tactccagac tataccggcc aaacgatagg
     1021 atacgttgta acccttcctg cccaaatgcc taattgctgg tcatctgaac taccaacaat
     1081 cgatctttat atcgaccaac ttcgcactgt tacaggcgtt tctaatgccc tcggattcat
     1141 cattgctgca ttgctaaacg cgtattctga tttaccccat gatttgaaaa ttggtctgcg
     1201 ttccttatca agttcagcag caatctactc gggtttgggt ttcgagcgag tgccgcaggg
     1261 ccgagacata aggtgcgatc atatgcatct aacgccagca aaccatcctg aattatggac
     1321 acaagagaat ggtgaatgga tctacttgcg aaactaatac ctgacgaaaa atagaaagca
     1381 aaacgccgac cagtagtcgg cgtttttacg tctcgttgaa taacgaatta ttcagctact
     1441 acgtttacga tcactttcgc gaatacttcg ctgtgaacct ggaagctcac ttcgtgttcg
     1501 ccagtggtac gcagaacgcc gttcggcaga cgaacttcgc tcttagccac ttcaacgcca
     1561 gctgcagtta cagcgtcagc gatgtcgcga gtaccgatgg aaccgaacag tttaccttcg
     1621 tcgccagctt tagacgcgat ggtaacagtt tccagtgcat tgattttctc agcgcgagca
     1681 ttagcagctg ccagaacttc agccagttta gcttccagtt cagcgcgacg tgcttcgaag
     1741 aattcaatgt ttttcttggt agctggaaca gctttaccct gcggtaccag gaagttacga
     1801 gcatagcccg ctttaacgtt tacctgatca cccaggctac ccaggtttgc tactttatca
     1861 agcagaataa cttgcattac cttatcctct caaagtcgta ttaatggacc gtgaccgatt
     1921 actgatggcg atcagtgtac ggcagcaggg acaggtagcg agcgcgtttg atagcgcgag
     1981 ccagctgacg ctggtatttt gcacgggtac cggtgatacg gcttgggaca atcttaccgc
     2041 tttcggtgat gtagtttttc agcgtagcga tatctttata gtcgatctct tgaacgcctt
     2101 ccgcggtgaa acggcagaac ttgcgacgac ggaaataacg tgccatatgg ctagtctcca
     2161 gaatctatca attcaatctg ctcggcatgc aaaaccattt tgctcagtcc gttctttgcc
     2221 ttgtggcatg aaatgaaccc ctgaacggtt atgcgactgc cgaccgttat actgtgagta
     2281 atggcctggt tttcgtgtcc gctaacaata acgggcattt ggcaccacgc ctgccggtga
     2341 aaaccggctt cctcctgcac agaacgatgc tcaagcacga actggcagtg aggaattcct
     2401 gatggactga cctttcgaag gggagtcctg cacacggtgc cggacaacac cagacggttg
     2461 gtcatcagaa attactcttc agaatcccca gcttcagcat catcagcggt ttcgtttgcg
     2521 aaatcatcgc gacgctcacg gcgctcgtct ttcgctttaa ccatcggaga tgcttcggta
     2581 acagcgtgct tggtacgcat aaccatgctg cggataacgg catcgttgaa gcggaaggta
     2641 gtttccagct catcgatcac ttcctgcgga gcttcaacgt tcatcagaac gtagtgtgct
     2701 ttgtgcagtt tgttgatcgg gtaagccagc tgacggcggc cccagtcttc cagacggtgg
     2761 atcttgcctt ctgcaccagt gatggcagca gtgtagcgct cgatcatgcc cggaacctgt
     2821 tcgctctggt caggatggac cataaaaacg atttcgtaat gacgcatcga attgctcctt
     2881 acggattatt cagcctcctg tctgggtcag ccgaatcccg gggaggcaag gaacgtgtta
     2941 aaggtcggct gaaaaatgac gcgttatagt actgacatac cccgctaaac tcaaggccag
     3001 ccgacaaata aatctcatct ttcaccaaat cgtactaaca cgctgatttg aagttcatca
     3061 cacttcatca tggtgttttt ttgaacaact gcatcagaaa tggtcaccat caacttcttt
     3121 atcattgatt aaaattaaat cagaagatca tcacagagga gagcacacaa agtcagccgt
     3181 tgaggaggta tctatgttca gtcgtgtttt agcccttctg gctgtgcttt tgctaagtgc
     3241 aaatacatgg gcagccattg aaattaataa ccaccaggcc agaaatatgg acgatgtgca
     3301 aagcttaggc gtgatttata tcaaccataa cttcgccact gaaagtgaag cacgtcaggc
     3361 attaaatgaa gagacagatg cgcagggcgc aacgtactac catccgatcc tgatacggga
     3421 accgggaagt aacggcaata tgcacgccag cgcggatatt tatcgctagc accaggtata
     3481 accaacgaaa cattgccata gtttgctttg cccccttcgc agggggcttt ttttgaaaaa
     3541 cggctgcgga attagaccag ttatctcccg aagaaggaaa tttccgcagc gcgtgtttta
     3601 cttctgcccg taataagcgt tgggaccgtg cttacgcatg aagtgtttat tcatgaggta
     3661 gctgtcgatg tgattcaatt gtgggttaat gccgcgggca atccacgcca tttttgccac
     3721 ttcttccatc accaccgcgt tatgcaccgc atcgtgagca tctttccccc aggcgaacgg
     3781 cccgtgctgg tacaccacaa tccccggtgt atgcagaggc tcggcgttgc ccagcgtttc
     3841 gataatcact ttgccggtgt tcagttcgta ctcaccctgc acctcttctt cgcttaatcc
     3901 gcgcgtacac gggatatcac caaagaagta gtcggcgtgc gtagtgccta acgccgggat
     3961 cgccagcccc gcctgcgccc atgcggtagc atgagtggag tgggtatgga caatgccgcc
     4021 aagcgacgga taacgacggt agagttcgag atgcgtcgcg gtgtcggaag acgggcgata
     4081 ttccccttcc accaccttgc cgctcatatc aaccaccacc atatcggccg ctttcatggt
     4141 ttcgtaggca acgccgctgg gcttgatcac caccagcccg cgttcgcggt caatggcgct
     4201 gacgttgccc caggtaaagg tcaccagccc gtagcgcggc agatccatgt tggcttcaaa
     4261 tacctgctgt tttagctttt gcattatgcc gcctccacca tgcccgcttt cgccatgcgc
     4321 gctttcaccc aatcacgcgc tttcgccact tctgccgccg ggtcttccgc cgtttcgctc
     4381 cacatctcaa tcaggtacgg cccgcaatag ccactctgtt tgagcgtttc gaagcaacgt
     4441 tcgaaatcca ccacaccttc gccaaacggc acgtttttga agacgcctgg tttggtgtct
     4501 ttcacatgca ccgcgacgat atgcccaatt ccggcctgca attccatctg cacgtcgttg
     4561 tcccacgccg acaggttgcc gatatccggg tagagctgga accacgggtt gttgagatag
     4621 tgcgcgtaac ccaacgcctt actgatggag ttcatcaacg ggtaatccat gatctccatc
     4681 gccagcgtca cctgcgcgcg gcttgccatc tcaacgctct ctttcaggcc gtcacggaaa
     4741 cgacgacgcg tttcgttatt ggcttcctga tagtaaacgt catagcccgc cagttggatt
     4801 acgcgaatgc caacgtcctg ggcgaactgg atagctttac gcataatctc cagcccctgc
     4861 gcccgtaccg cgtcatcttc gctgcccagc gggaaacgac gatgagcaga aaggcacata
     4921 gacggtacac gcacgccggt ttcaacaatc gcattcacca gcgccagacg ctgctcgcgg
     4981 ctccagtcga ggcgcgacag gcgatcgtct gtttcatcta ccgacatctc gacaaaatcg
     5041 aagcctaacg tttttgccag ttgcagccgt tccagccagc actccccggc ggggagcgct
     5101 ttttcataga tgccaagcgg gatttgtttg gacaacatat ccgctcctta gccccacagt
     5161 tcggcaatgg aacgtttgaa ctgacgcgca gcttcaaccg gagaagcggc atcacggata
     5221 ctgcgaccag cgataaacac atgaatcgga atgcctttga acagcggcag atcttccagc
     5281 gccaggccac cggtgacggt cactttaaag cccatatcgg acagacgttt gatcgcggta
     5341 atgtcagctt cgccccacgc cacgcctgcg gcctgcgcgt cacggctgcg gtgataaacc
     5401 acctgctgaa tacctgcgtc gcgccactgt tgcgcctgtt cccaggtcca gtagccagtc
     5461 agttcgatct gcacgtcgcc gttaaactct tttgccacgt ccagcgcgcc tttggcggtg
     5521 ttgatatccg cacagcaaat cacggtcacc cagtcagcgt tggcttcgaa gcacatacgc
     5581 gaaaggattt tgcctgcatc ggcaattttg gcgtctgcca gcacgatttt gtgcgggtaa
     5641 agcgctttca gatcgcgaac cgcacgcacg ccttcgccca cgcacagaat ggtacccact
     5701 tcgataatgt cgacttcttc ggcaatcagg cgagtggttt cataggcgct atccatagtc
     5761 tggttgtcca gcgcgacctg caacatcggt aatgacatgt tcaattcctt cttaagctgc
     5821 cgcgttggtg cggtcaatta aatccagtac ttcctgctcg gtacggcagg cacgtaaacg
     5881 gtcgaaattc tcttcatctt caaacagatt gacgatctgc atgatgccca cttcctggtg
     5941 agtgttggca tcgaccgccg ccatggtgat gaggatatcc accggatcgt tatcttcgtg
     6001 gttgaactcc agcggctttt tcagcgtcac tagcgcaaaa ccggttttct taacgccctc
     6061 ttccgggcga ccgtgcggca ttgccaggcc tggtgcgagc acaaagtacg gaccgtgctg
     6121 ttcaaccgca tccagaatcg cctggtagta acgcggctcg acaacatccg ccgctaccag
     6181 caggtcaacg ccgattttta ctgcgtcctg ccatgtctca gcttcagcct gcaggcggat
     6241 ggatttattt tccgccagcg aatcacgtaa tttcatggcg cgtccttact tcacatcctg
     6301 cgggaaatgc gctttgatca cttccagcag tttcgggcca aagtcagcag gagagagcat
     6361 gttgcgcacg ccaaccacgt atttgttgcc ggtcacggtg atttcgcccg caatgtgcgt
     6421 agaagcgatg atgatatccg cgccgctcaa ctcgcttttg tattcgccaa ccgcgcagct
     6481 gtttaccgtg tggtcaatgt ttgattgggt taaaaactgg tccactttca tcttcatgat
     6541 catggaactg ccttgtccgt tgccacacac agccagaata cgtacggtca taatcaaaac
     6601 tccttattaa gcagactgtt ctgccagttg tttttctgca tcttcttctg cgcgcagcgc
     6661 gcggccagcg aagaacatat aagccagtgc aattacaatg atgacggcca taaaggcgat
     6721 accgatggag aagaagccct gcatcatcgg tggtgccaga atcgaccagt ccgccatacc
     6781 catccaggca ctcataccgg taagtttcac cgcccagacg caaccaaaga tttcgatcat
     6841 ccccatcacc agacaaatct tcagcgccgc acgccagccg ccgaagtggt tagcgaacac
     6901 gccgatggtg gcgttggaga agaacatcgg gataaagccg ggaataatca ggatcgagga
     6961 accgcaggcg accaggatgc caactgcaat cagctgaccg atggtgcccc acataaagcc
     7021 ccagaccacg gcgttcggcg cgaagctata gatagccgca cagtcaatcg ccagaaccgc
     7081 accagggatc aggcgctggg agataccgtt aaacgcttca gagagttccg ccacaaacat
     7141 gcgcacgcct tgagtgatga tgaagatcgc caccgcgaag gagaaaccag tttgcaggat
     7201 gtagaccgtc cagttcactt tgcctgccat cgcctgcacg gtgtcgatac cgaaggagag
     7261 cagaatggca ccaaagaaga tggtcatgac aatcgccgtg gagacaatgt tgtcgtggaa
     7321 aatattcagc cagcctggca gtttgaggtc ttcaacgctt tcctcttttt tgccgaggaa
     7381 cggcgcaacc ttataggcaa tccatgatgc aaactgttgc tggtgaccga tggagaaacc
     7441 gcagccgtcc gtcacttcct gagtcggctt gtacatcatg ttggaggtaa tgccccagta
     7501 gagcgaaacc agaatcgccg tgcagataat ggtcgtccac atggagtagc cgaaaataaa
     7561 caacgtaacg gcaatcagcc ccgcctgctg gaacatgata tgaccagtca gcatgatggt
     7621 acgaataccg gtaatgcgac gcagcagcac gtaacagatg ttcagtgcca gcgctaacaa
     7681 caccgcgtaa cctacccagc tataggcatc gcccatgcgg tcgatggttg ccatcatcga
     7741 tgcgtaagta tcggaaattg cgccgttaat gccgtagact tcggacattt tcgccaccac
     7801 cggtttgaag gtgctggtga ggatgccgga ccctgcctgc aacaacatga aaccaattat
     7861 ggttttaatc gtgcctttaa taataacgct gacacttttg cgcagtagga tgtagcccag
     7921 acaggtcaca atacccagca acaacggggc attggtcatg acctggttaa aaaacacggt
     7981 aaagatgttg tagaggatct ccataacgat ctcctgaaga agaggtaacc gggtattcca
     8041 cacacccgga atgttgtgca ctcactctaa ttttcaaaag taatcacaac aagattattt
     8101 atgattaaat gtgacgcgac ccgcaaataa ttccacatgg atagagtggg attcacacaa
     8161 cgaaacaatt attcatttaa attcattcag ttaattgaat tacacattcc attaaatctt
     8221 tccatactca aaatcaacaa caacttccct ggcaaaaagt caaaaaatgg tcattgcctt
     8281 cctgaaaaaa tagtgtcagg attaatcaaa accaatcaca tattgattcg aattgaacta
     8341 tcatgaaggt aaatggcgat gagtaaagtg aaaagtatca cccgtgaatc ctggatcctg
     8401 agcactttcc cggagtgggg tagctggctg aatgaagaaa ttgaacaaga gcaggtcgcc
     8461 cctggcacat ttgcgatgtg gtggctaggc tgcaccggga tctggctgaa atcagaaggt
     8521 ggcaccaacg tttgcgttga tttctggtgc ggcactggca aacaaagtca cggtaacccg
     8581 ttaatgaaac agggtcacca gatgcagcgc atggctggcg tgaaaaaact gcagccaaac
     8641 ctgcgcacca ccccgtttgt tcttgatccg tttgcgattc gccagatcga cgcggtactg
     8701 gcgactcacg atcacaacga tcatatcgac gttaacgtcg ctgctgccgt gatgcagaac
     8761 tgtgctgatg acgtaccgtt cattggaccg aaaacctgcg ttgatttgtg gattggctgg
     8821 ggcgtaccga aagagcgttg catcgtggtc aaacctggcg atgttgtaaa agtgaaagac
     8881 attgaaattc atgcgcttga cgctttcgac cgtactgcac tgatcaccct gcctgccgat
     8941 caaaaagcgg ctggcgtact gccagatggc atggacgatc gcgcagtaaa ctacctgttc
     9001 aaaacccctg gcggttccct gtatcacagt ggcgattccc actactctaa ctattatgcg
     9061 aagcacggta acgaacatca gatcgatgtg gcgttaggat cgtatggtga gaacccgcgc
     9121 ggtattaccg acaaaatgac cagcgccgat atgctgcgta tgggtgaagc cctgaatgcg
     9181 aaagtggtta tcccgttcca ccacgacatc tggtcaaact tccaggccga tccgcaagaa
     9241 atccgcgtgc tgtgggagat gaaaaaagat cgtctgaagt atggcttcaa gccgtttatc
     9301 tggcaggtgg gcggcaaatt tacctggccg ttggataaag acaacttcga gtaccactat
     9361 ccgcgtggtt tcgatgattg cttcactatt gaaccggatc tgccgttcaa gtcattcctg
     9421 taatctgtcg tttataccgg atagcgtgga gtagttcgcg ctatccggcg tagtatttcc
     9481 tggctatttc aaatatcatc taaaaaaatc aaattttatc ggaatagctc atgactgaag
     9541 cacaaagaca tcaaatcctc ctggaaatgc tcgcacaatt gggctttgtg accgttgaga
     9601 aagtcgttga gcgtctggga atttcgcctg ccactgcgcg acgcgatatc aataaacttg
     9661 acgaaagcgg caaactgaaa aaagtgcgca atggcgcaga agctattacc cagcagcgcc
     9721 cacgctggac gccgatgaat ctgcatcagg cgcagaatca cgatgaaaaa gtacgtattg
     9781 ctaaagcggc ctcgcagctg gttaatccgg gcgaaagcgt agtcatcaac tgcggctcca
     9841 ccgcgtttct gcttgggcgg gaaatgtgtg gcaagcctgt gcaaatcatc actaattatc
     9901 taccgctggc aaattacctg atcgatcagg agcatgacag cgtgatcatt atgggcggac
     9961 aatacaacaa aagtcagtcc atcactttaa gcccgcaagg cagcgaaaac agtctctatg
    10021 ccgggcactg gatgtttacc agcggcaaag ggctgacagc agaagggttg tataaaaccg
    10081 atatgctgac ggcaatggca gagcagaaga tgctaagcgt ggtagggaaa ctggtggtac
    10141 tggttgatag cagtaagatc ggcgaacgcg cgggaatgct ttttagccgt gccgatcaaa
    10201 tcgatatgct gatcaccggc aaaaatgcta acccggaaat cctgcaacaa ctggaagcgc
    10261 aaggggtcag cattctgcgt gtttaaagat gctggcggaa aaatgtcacg gcagcatcta
    10321 acgcctcagg cgtaatgcgg tgacgcacgc ctggctgcca tgaacaggtt agcagtttat
    10381 cccgtcccgt ctcgcttaag gcctgctgca aacgtagtga ttcgtcggca ggcacgacgt
    10441 catcgtcaag gccatgccac agcagtagag gtctgtcacc aagttgttcc aggtggtttg
    10501 tcgcttccca ctctgccagc ggcgcgacaa tgttattgaa ttcattctgc tgtgctgccg
    10561 tttcaggtat cagcggtgga aacagtgaac gggcgagcga tgtaaaatag cccgatccca
    10621 tcatgctggc ggtacatttc accgtggggt ggcgagcggt aattcccagt gccgtcatcg
    10681 cgcccatcga cgcgccaccg actgccagac ggtcagcaag cagccagttt tcttcggcta
    10741 ttgccgcacg taacgtagtg aattcctgca tactttgtag caaaatttgc caaaattgat
    10801 ttaaccgccg cgctgcgtcg cccctaaaac ggctaccgtg atcgggcgca tccggcatga
    10861 tcacccgcaa accagcctgc gccagcgcaa cggcaaaata gctatacacc agactggacg
    10921 aagtaaagcc gtgataaaaa atcgcgcacg gtaacggggt atctttttgc ccgacaggat
    10981 aagcatgaag aacgggaata tctgccagct cgcgtgattc tatttcaatc atctgtctct
    11041 cctttttgtc tggtatgaca tgaagaaatc cttagcacaa gtttctgctg gcggtattag
    11101 ttcataatgt tgagatgtgg gttacgcttt cgtcacgttt tcattcgaaa atcgcgtacc
    11161 aggtaacaat tcgggaacat tcccccaaag ttaaattaat aaggcactac actatggtta
    11221 gcaggaaacg taatagcgtt atttaccggt ttgccagttt attattggtg ttaatgttaa
    11281 gtgcctgtag cgcactgcaa ggtacgccac agccagcacc gccagtaacg gaccatccgc
    11341 aagagattcg ccgcgaccag acgcaagggt tacagcgaat aggtagcgta agcacgatgg
    11401 ttcggggttc tccggatgac gcattagcag aaatcagagc gaaagctgtc gctgcaaaag
    11461 ctgattatta cgttgtcgtc atggtggacg aaaccatcgt gacaggacag tggtattcac
    11521 aagccatttt gtaccgtaaa taaatcagac aacttacata gtcagcttta cattgctttg
    11581 cgtccctatg cgtttacctg tgcacaataa attaccggct ggaagcctgg gcgtttatta
    11641 ccgcgatgga atgccctgca acatgtgggg aaacgaaaaa tggagctgac gatgaaacaa
    11701 ttacttgcct caccctcgct gcaattagtg acttatcctg cgagcgccac ggcacagtct
    11761 gcagaattcg ccagtgctga ctgcgtgacg ggcctgaatg aaataggtca gatctcagtt
    11821 agcaatattt caggggatcc gcaggacgtg gaacgtatcg tggcgttaaa ggccgatgaa
    11881 caaggtgcgt catggtaccg cattattacc atgtacgaag accaacagcc tgataactgg
    11941 cgtgtacagg cgatactgta tgcgtgagtg tcatctgaag cgaatgaaca atgccggatg
    12001 cggcgcgagc gccttgtccg gcctacaatt ttcacatatt tcatttagtt aatcgaaacc
    12061 agcgtcgcat ccgtcgtata cgcttacaaa cacactcccc ccgtcgcccg cagcaataaa
    12121 tcattctgga tctgctctga caaccgcacg ccaccgcgcg tatttaacat cacctgacac
    12181 cacgcctgtg ccattggcgg agaagcatat ttcaacattt gcgcaccgca gcccagcagg
    12241 aatagctgat gagtaatctc tcgccccagt tcttcagctg gcttacgcaa ctgctgctgt
    12301 aaacgacgaa ccgcgcgatc aaaataacga tcttgccctt tcacttccat aaacgcttcc
    12361 gacagcaagt cgtatactcc cgcttgctta ttaagaacgc gcagcacatc caggcacata
    12421 atattgccgg aaccttccca aatactgttt accggcatct cccggtaaag ccgcggcagc
    12481 tcgctctctt cgcaatagcc aataccgccc agcacctcca tcgcctcagc cacaaacgga
    12541 atgccacgtt tgcagatcac aaatttcgcc gcaggcgtaa ataagcgcgc ccacagggct
    12601 tctttggcat cggcacgccg atcccacgct cgcgcaagac gaaacagcaa cgccgtttgc
    12661 ccttcaagct gaagcgccat gcgacttaag acatggcgca taaggggctg ttctattaac
    12721 ggattaccaa atacatggcg ttgatgagcg tgatatatcg ccagcgagaa cgcacggcgc
    12781 atcatggcat ggctacccag ggcgcaatca aaacgcgtca tcccacccat tttcaggatc
    12841 agacgaatcc cttccccttc ctgacccaac aaccagccaa tggcatcctg aaactccact
    12901 tcgcaactgg cattggaacg attacccagc ttgtctttca gccgctcgag gcgaatcgcg
    12961 ttgcgttgcc cgtcaggcaa aaagcgcggc acaaaaaagc aggacagacc gcccgcggtc
    13021 tgcgccagca ccagatgcgc atcgctttgt ggcactgaga aaaaccattt atgccccacc
    13081 agccgataag agccatcttc cagacgctct gcgcgggtgg tgttgctcat cacatcggaa
    13141 ccgccctgct tttccgtcat tcccatgcca atcaacaaac cgcgtttttg cccacctggc
    13201 aataagtgag aatcgtagcg atcgctcaac agcggcgtgg tccagtcctg aaacggcgtg
    13261 ggtaacatct gtaacagcaa tggcgtggcg gcaaaggtca tggttatcgg acataacgac
    13321 ccagcctcaa cctgcgcatg taacataaaa cgcgccgcgc gcgccacaaa tgcgccggag
    13381 cgagcgtctt cttcccaggc caggttgtgt acccgattgg tacataacgc ctgcatcagc
    13441 aggtgccatg cgggatggaa acggacatcg tccagacgcc gcccctgggc atcgtagcgc
    13501 aatagttcag gtgggtttac attcgccagt cgccccagtt caagggattc agccgttccg
    13561 agttgctgac caatactggc gagaaaatcg ctatcccagc cagcgccttc gcgtgttacc
    13621 gcttcgcaga gcgcgccatc agataagtat aaattgctgt tatttaaggg tataggttga
    13681 ttaaaaacag tgtgagtttg ccagtgcact gtgtctccct ccatcaatgg cagtcaccat
    13741 tagtatggtc actgccacgg attcccgaca gaaagagatt gctaaaacat tcttaacccg
    13801 caatgtagtg cggaacaaaa cgcgaagtgt ctttagtgat cagcgtgtta tcttcgcgga
    13861 tacccatacc gcacgcttca tcatcgacaa tccagctacc aatcagcgtg taactatcgc
    13921 caaatcgcgg tagaggttga aacgcctgat agatcatcgg ttcatcggcg taatcaccgt
    13981 cagcgtggtc aacgacgtta ttctgaccgt cgaaaatggt gacgttaccg ccctcgcgcg
    14041 agtagatcgg tttacgcaca tagctttcgc cagcggcgat ctgcagtttt tcgccctcaa
    14101 accaggacgc cagaagatta ggatgaccag gaaagaagcg ccaaagcagc ggcattagcc
    14161 ctttgttact caagatactt ttccataacg gctccaccca ttgctcgcgg cgcttgcgca
    14221 gcagcggacc gttatcgtca cgcatcatcc actccagcgg gtacagctta aatgcacgct
    14281 ggatgacatt atcatcaaga tcggtcagta caccgccgac gcccaaaccg agatcttcaa
    14341 tgtagataaa ccgcgactcc tgccctgcct gctgggcgca gtcctgcaag tacagcacgg
    14401 tactcctgtc ttcgtcagtg tcctgacaac agcaaaaata aaacggctcc cgactataaa
    14461 gctcgctaaa gcgcgaaatc aggcgttcct gaatggcatt gtactgatcg gtatcacgcg
    14521 gaataacgcc gctgcgccgg gcatcctcca gccacagcca ctgaaaataa gccgattcat
    14581 acaacgaggt tggcgtatcg gcgttgtact ccagcagctt caccggcgca ttgccacacc
    14641 aggcaaaatc catccggcca tacagcgaag gatcgcgtgc gcgccagctt tcagcgatca
    14701 catcccagta caacggaggg atcgccagtt gcgtcaggat ctcttcgtct ttaaccgcgc
    14761 gatccaccac ctcaaggcac atctgatgca gttccgcagt cggtttttcg atctgctctt
    14821 caatctggcg cagagtaaaa cggtaagccc gactctcgtc ccaatagatt tcattgtcga
    14881 tgatatgaaa gtcgaaaccg ttgtcagcgg cgatctggtc cagatcccgc cgcacaggaa
    14941 cgttgtgtct cagcatgatt agcctcccca atgcccacgg gcgctggaag aacgaccata
    15001 gccgccgcga gaaacggttg acgctttttt ggtggtaaag ccgcccgaag agtaagactc
    15061 ttttttgcca gaaccggagc gccaggagta atcaccagaa gtgttgcgcc agacggggcg
    15121 cgaagcaaaa gaggaaccac cgctgttata cacaaactgc tcatcgcgat ctttacgaat
    15181 aacccggctt aacaaaaatc cagaaacgac cgggatccaa ctctgttcaa cgttgtcgta
    15241 gtaacaattt tcatacttag actgacaatt ctgctgagtc atattcttcg gaacatcggc
    15301 ataaaacgcc gctttggcgt tgttccagcc atgagcacag atatctgaat tattaccgtc
    15361 atcaatacaa tcctgcacag tcgagtaaaa cgttccgtcg ccgtcgttat cgacatcgcc
    15421 actatcgcca caacctttca atacgaaaaa agcggcaccg cccattagcg ccaacgttaa
    15481 gtatttttgt gcgtagcgat tgcgacgcgg ttcaaaagga ttattcggtc tgccaatgcg
    15541 actgatcgct ccgtgaccaa ttttactgtt atttcttgat ttgcgttttc tggccatgat
    15601 cgtccattac catgtcatac aagctgcgtt aaaaatacca ccagccagtg cggcggttcc
    15661 cataaacatt cctgctgcgg tattgtgatt aataattttt tcgcttaatg cgggcatata
    15721 aagcctgacg ccagcaaaaa cgagtaattg aatcaccaga gcaatcccgc cccaggcgaa
    15781 atagtctgga atactcaccg cattgattgc cgcactggat aaggggatca cgtaacccaa
    15841 caatgtaccg ctgaacgcca gtgacgccgc ggtattattg tttttgatta actgccattc
    15901 gttgtgcggt gtaattttag aatagataaa cagaaaaata atcaccatag ccacgccaat
    15961 aaaaaaatag gcactaaagg caagaagaga atccagtata tgcattgtaa acgtccttgt
    16021 aattaaccaa taatatgaag tgatgtcagc gggatatcga cacccaacgc gcgcgaaaat
    16081 aaccactctg gctcgccgag atcgttaaaa gattcctcac catttagcag caaatattcg
    16141 taagtatctt cggttacttg ccgttggtag cccatggtaa aattatgtac ttcccatttg
    16201 gcatggtttt gattttctac tttttccagc atgtagacgg gttcgatatt tcccggttct
    16261 tcgctgttaa aaaatcgcag ccagcgtttt tcctgccagt ttaaggtcat tgcccccatc
    16321 gctttggcat tgatcgtctc gcgccagtga ctctctttac tgataccgta gctctcttca
    16381 tagacaaaga gcttgatatc atcaatgtca tcaatatttt cgccgccggt ggtattgatt
    16441 tgcagaaact catcgcctga agtgtagtag cggaaaatct ggctaccgcc gcccagatcg
    16501 atgcggctga cggcggctac cgtaaattcc tcacccggca gcgcaatcag caattcatct
    16561 tccagcaaac gaaacgctaa cgtgtcgagc gtaaaaccac tattgagatg cagacccagc
    16621 ggaccgcgag cgatagcagg cttattatcc ttgccaaaca gacgctggaa aaaaccagac
    16681 atattttatc cttaaaatag ttgccagcct tgcagtgagg ctggcaaaaa aattactcgc
    16741 cctgttggcg ttgcagtctt gctaatacat cctgggcgct acttttattg ctaccgccta
    16801 ttccggcttc cgccagcttt tcatcaagat cgcgaccgtc tgcgactttt tccaactgtg
    16861 cagcagcatc cagacgagcc tgacgttcgg cctgacgcgt ttgcaggcgt tttaaggatt
    16921 ctgccgctgt cgaaacgctg gaagatgcgc caacggtaga ggtcgttaca gcctgttgtg
    16981 cacgctgcat ggcttcagtg gctttaacga cttccatttg ctgctcaaac tgagcgatac
    17041 gctgcgctgt cgctgtaact gctttttcca cgccatcacg agagacttcc agattcgaca
    17101 acacttgctc ttcagcggta atgagattct caaggcgcgc gatttcttca gcaacttcgt
    17161 taatcaacga cggattaacg ttcttgctta acgctttcag cgcacgagct tccagactgg
    17221 ctttgcgctc acgtaaatct ttcagcttat cgtgacttaa tttcacccgc gccagcagat
    17281 caacgcgaga ttttccggct ttatcgagtt cagctttagc gtcacggata tgctgttcca
    17341 gcatgcgcac gccctgggtt tcttcaatgg attcttccgc ctgggagata aacgatttcc
    17401 ccagcgtaaa taaacttttt aaaattccca taaatacccg tccttgtaat tagagtgaat
    17461 attcttctgt aatttcagcc agatccaatg cgttatctac cagcgaggtt atctcaagca
    17521 ggatatcttc aagagaagat ttaagcgata acgccccgaa aacaatgtaa tactcttcat
    17581 gttgcacgct ggagatccct accgatgaca acggcatcat cttctgattt cttaataaga
    17641 aggtattaaa ttcatctgga ttgctgatac tactcaccgg acaaataaag gtttcgataa
    17701 tcatttggcg ggaagtaaaa agaatattga tttgcaaatc gccataatca ttcattttaa
    17761 taattaatgc gttctcgcta tttgttacat caatattttg ttcaggtaca gtttgcagcg
    17821 ccgtcgccag cgccaacgga ttccatgtca tatattattc catatagatt tagtttaaat
    17881 attaacaaaa tgaatatttg cagtacgtaa ttatcttacc agctaaacac aaaaaaaaac
    17941 catccaaatc tggatggctt ttcataattc agaggaacta gctgcgctgt cgcaccgctt
    18001 caaataagca aatccctgtc gcaaccgaaa cgttcaggga agaaacgctt cctgccattg
    18061 ggatgctgat caactcgtcg caatgttcac gagtcaggcg acgcatacct tcaccttccg
    18121 cgcccatcac cagtgccagc cgtccggtca ttttgctctg ataaagtgta tgatccgcct
    18181 cacctgccgt accgacgatc cagatattct cttcctgcaa catacgcatg gtgcgcgcca
    18241 gattagtcac ccgaatcagc ggaacgcttt ctgccgcacc acaggccact tttttcgccg
    18301 tggcgttgag ctgtgcggag cgatctttcg gcacaatcac cgcatgaaca ccagcggcgt
    18361 ccgcgctacg caggcacgcg ccgaggttgt gcggatcggt tacgccgtcg aggatcagca
    18421 ggaacggttg atcgagcgaa gcgatcagat ccggcagatc gttttcctga tactgccgtc
    18481 ctggcttcac acgggcgata atgccctgat gcacggcacc gtcgcttttc tcgtcgagat
    18541 actgacggtt cgccaactgg ataaccacgc cctgggactc aagggcgtga atcagcggta
    18601 acagacgttt atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt tcaggggcgc
    18661 gctccagcag ggcctgcact gcgtggatgc cgtaaatcat ttcgctcatt aatgtactcg
    18721 ttgtttacgt tttgccggga gcgccacgct tgcccggcct acggataatt caccctcttc
    18781 tgtttgaaga gggttatgat cactctgcca cttttttctt cgccgcacgc ttcgctttgg
    18841 tcgctgcggc tattttctgc gttttagccg atggcttttt cgcttttctc gcgtctttct
    18901 tcgccgcttt cggcttcggt tttttctcgc cgcggaaggc actgtctggc tcaaagttta
    18961 cctttttacc gacctgacga cgcttgccgc cttttttgcc agcatcgcct tttttcgctt
    19021 tctcgcgcgc cgttttaccg acgttgcgcg gtgcgcgttc gctggagatc aggctaaagt
    19081 cgattttacg ctcgtccata ttaaccgctt cgacgcgaac ttccacgcga tcgcccaggc
    19141 gataagtctg gccgctggat tcacccatca ggcgttgccc tacctggtca aagcgatagt
    19201 agtcattgtc cagcgaagag acatggacca gaccatcaat gaacaagtcg tccagacgga
    19261 cgaagaagcc aaagccagtg acgctggaaa ttacgccttt aaagacgtta cctacctggt
    19321 cgagcatgaa gtcacacttc agccagtcag ctacatcgcg tgttgcttcg tcggcacgac
    19381 gttccgccat cgaacagtgc tgacccagtt gcagcatctc ttccatcgaa taatggtaac
    19441 caccggtttc agtggtattg ccctgatgcc cctgctcttt cgccagcaga tacttaatgg
    19501 cgcggtgcag cgtcaggtca ggataacgac gaatcggcga agtgaagtgc gcataggact
    19561 gcaatgccag accgaagtga ccacggtttt ccggatcgta aatcgcctgt ttcatcgagc
    19621 gcagcagcat ggtttgcagc atttctgcat caggacgatc ggcaaccgac tctagcaact
    19681 ccgcgtaatc acgcggttcc ggcttgttac cgcccggcag ttccagcccc agctccgcca
    19741 gcactgaacg gaaagaggta atcgcttcgg tgctcggctt atcgtgaata cggaacagtg
    19801 ccggttcttt cgctttctca acgaaacgcg ccgccgagat attcgccaga atcatacact
    19861 cttcaattaa tttgtgtgcg tcgttacgct gggtctgttc gatacgttca atacggcgtt
    19921 cagcgttgaa aatgaacttc gcttcttcgc tctcaaatga gatcccaccg cgttcttcac
    19981 gggctttatc cagcacttta tagaggttat gcaactcttc gagatgctta accagcgggg
    20041 cgtactgctc gcgcagatcc tgatcgccct gcagaatatg ccagactttg gtgtaggtca
    20101 gacgtgcgtg agagctcatt accgcttcat agaatttgta gccagtcagg cggcctttcg
    20161 acgaaaccgt catctcgcac accatacaca ggcggtctac ctgcgggtta agcgaacaca
    20221 gaccgttaga gagcacttcc ggcagcatcg ggataacctg cgaagggaag tacaccgacg
    20281 taccacggtt acgcgcttct ctgtccagcg gcgttggagg gcgcacatag tagctgacgt
    20341 cggcaatcgc gacccataaa cgccagccgc cgccgcgttt tttctcgcag taaaccgcat
    20401 cgtcaaagtc acgggcgtct tcgccatcaa tggtgaccag cggtaaatcg cgcagatcaa
    20461 cacggcccgc ttttgcttct tccggcactt cttctttcag cccggcaacc tgttgctcaa
    20521 cagcctgcgg ccagatgtac ggaatttcat gggtacgcag agcgatatca accgccatgc
    20581 cggtgcccat attgtcgccc agcacttcga cgattttacc caccgctttg gtacggcgag
    20641 tcgggcgttg ggtcagttca acaaccacca caaagcccat ccgcgcgccc atgatctgat
    20701 cgggcgggat taagatatcg aagctcagac ggctgtcgtc aggaaccaca aagccgacgc
    20761 ccgcttcggt aaagtagcga ccaacaatct ggctggtttt tggcaccagt acgcggacaa
    20821 tacgcgcttc acgacgacct ttacggtcag cgcccagcgg ctgcgccagc acctgatcgc
    20881 catgaatgca ggttttcatc tgctcgctgg agagatacaa atcatcttta cgcccttcaa
    20941 cccgcagaaa gccgtagcca tcacggtggc caataacggt acctttcacc aggtcgaggc
    21001 gttccggcag cgcatagcac tgacgacgag tgaagaccag ttgaccatcg cgctccatcg
    21061 cgcgcaggcg gcgacgcagg ccttcaagct gctcttcgcc ttcaatgtgc agttctaccg
    21121 ccagctcatc acggctggcc ggtttttcac gtttggttaa atgttcgagg ataaattccc
    21181 gactagggat gggattcgcg tatttttcag cttcgcgttc ctggaaagga tcttgtgaca
    21241 tctcggttcc tccgttgtca tctctgatga agattttcgt cactccacca gcaataattt
    21301 ataaagcggt tgattctctt caaccaaatc ggcaagcgtg tagttatcca gttccgtaag
    21361 aaaactttgc acggccttag aaagtgcctg tttcaaccga caggcaggtg taatgtggca
    21421 aaactcactg ctgcaattca ccagcgataa gggctccagc tcgcgcacca catcaccaat
    21481 acgtatcgca ctcgccggtt tacccagacg aatgccgcca ttttttccac gaacagcagt
    21541 cacgtagccg gcacgactaa gttgattgat tattttgacc atatgattac gggagacgcc
    21601 gtagacgtca gtcacttcag aaatactggt catccgccct tctggcaatg acgccatgta
    21661 gatcagcgca cgtaatccgt aatcagtgaa actcgttaac tgcacatcaa cctcaaaagg
    21721 gaaatcggga aaaaatacat ttatattgat gataaaccag ccacaagctg tgtcgctaat
    21781 ttatttcagt tcggggaagg aaaaaagcga ggatttaaca ctgtgtcgga aagcgtaatg
    21841 gcttaaccga cacagcaatg tatcacactg ttagcccggc aggcaaaata tctgccaggc
    21901 gtaccagaat tacgcgtcga acgggtcgcg cagaatcatg gtttcagtac gatccggacc
    21961 ggtagagatg atatcgatcg gcacaccggt cagctcttca atacgcttga tgtagttcag
    22021 cgccgcctgc ggcaggccgc tacgatcttt cacgccgaag gtggattcag accagcccgg
    22081 catggtttcg taaatcggct ctacaccttt ccagtcgtca gctgccagcg gagtggtagt
    22141 cacttcacga ccatccggca tacggtaagc cacgcagagt ttcacctctt tcaggccatc
    22201 cagaacgtcc agcttggtca ggcagaagcc agacagggag ttcagctgta ccgcacgacg
    22261 aacggcaacg gtgtccagcc agccggtacg acgacgacga cccgtagttg cgccgaattc
    22321 gttaccctgc ttgcagagga actcgccagt ttcatcaaac agttcagtcg ggaacggacc
    22381 tgcacccaca cgagtggagt aagctttgag gatacccaga acgtaatcaa cataacgcgg
    22441 gcccaggccg gaaccggtcg ccacgccacc agcagtggtg ttggaagatg ttacgtacgg
    22501 ataagtaccg tggtcgatat ccagcagcgt accctgagca ccttcgaaca tgacgaaatc
    22561 gccacgctga cgcgcctggt cgagcaggtc agaaacgtca accaccatag aagtcaggat
    22621 gtcggcaaca gccatcgtat catccagaac tttctggtaa tcaaccgctt cagctttgta
    22681 gtagttaacc aactggaagt tgtgatattc catcacttct ttcagttttt cagcgaaggt
    22741 ttctttgtcg aaaaggtcgc caacacgcag accgcgacgt gccactttat cttcataagc
    22801 aggcccgata ccacgaccgg tggtgccgat cgctttcgcg ccacgcgctt tctcacgcgc
    22861 gttatccagc gcaacgtgat aatcaaggat cagcggacat gcttcagaca gcagcagacg
    22921 ctcacgaacg gggatgccac ggtcttccag ttctttcatc tctttcatca gcgcggctgg
    22981 agacagcaca acaccgttac cgatgatgct ggttacattc tcgcggagaa tacctgatgg
    23041 aataagatgg agaacggttt tttcaccgtt aattacgaga gtatggcctg cgttgtgacc
    23101 gccctggtag cgtacaacat atttagcccg ttcagtcaga agatcgacga tcttaccttt
    23161 accttcgtca ccccattggg tgcccagtac gacgacgttg ttacccattt ttcaaaatca
    23221 ccgtttgctt aaaaatggat tctaccatcg ctttttcaga gttacagcac tttttgcact
    23281 caaaaatgac ctcgatcagt ctgttttttg ttcagccaat cgttttcctc aacatgtagt
    23341 agaccacaac gcccgcaacc acaagtccac cgccaaaacg acgtaaaata ttatcaggca
    23401 aattggtcat cgcagagatc atcttcttcc atgccttcgg gtaaagcatc ggccctaaac
    23461 cttcgagcac caaaaccagg gcaagcgcca gccagattgt cgaattcatt cattatcctt
    23521 atagaaaaag aaaaccaccg acatccctga ggatgcggtg gctttattga cctgtaccgc
    23581 agtcgttata ttaacgcgtt gcggaagtcg gcgtcttcat gtagcggaag aaatcgctat
    23641 ccgggctcat gaccatcacg tcctgattgc cagagaagct gttctcataa gcacgcaggc
    23701 tacggatgaa tgcgtagaag tccggatctt tactgaatgc atcagcaaac agtttggctg
    23761 cttcggcatc gccttcacca cgcatgatgc ggccctgacg ctcagcttct gccagcgttc
    23821 tggtcacttc atagtcggca gtcgcgcgca gtttttccgc ttcttcctga ccttgtgaac
    23881 ggtgacgacg cgctaccgct tcacgctcgg cgcgcatacg gttgtagatc gcttcagaca
    23941 cttcggtcgg caggttgatc tgcttgatac gcacatcgac aacttcaata cccagcgccg
    24001 ccatactgtt cgggttgatg accggaactt tgcccttcgt ctcagccgtt acgcgctctg
    24061 ccgcttcggc aatggcgtta tctgctgccg gggtagtaac ttcatcttct gtacccgcag
    24121 aaccggagtt cagcgcgtca cgtacttcga gggtcagacg accacgggaa tcggtgacga
    24181 tgtctttcac atccagacga ccaatttcag aacgcagacg gtcagagaac ttacgtttca
    24241 acagcacttc cgcttgcgaa atgtcgccac cgcccgttgc caggtagtaa cgactgaaat
    24301 cgctgatgcg ccatttgatg taagagtcga cgatcaggtc tttcttctct ttggtcacaa
    24361 agcggtcggc ctggttgtcc atggtctgaa tacgtgcgtc gagcattttc accgtttcaa
    24421 tgaacggtat cttgaaatgc agacccggct cataaaccag aggtttgttg tcatcgtcac
    24481 gcagtacctt accaaaacgc agcgtaatgc cgcgctcacc ttctttgacg acaaagacag
    24541 acatgtaaag cactaccagc acgatgatga taatcgcgat aactgactta cgcatcgtta
    24601 ttccccctga cgctggtagt cgttacgctg cgcgttggcg cggcgttggt ccataatatc
    24661 gccctgactg gtggacgacg tgttgcttgc tccactggtt gtggaggaag aggctggcgg
    24721 cagacgcagc agattgctgg caccgttatc gctcttcgcc gcaggggcgt taccaccttt
    24781 cagcatctgg tctaacggca gaaccatcag gttgccacct ttatcgttaa ccagcacttt
    24841 gcgggtgttg cccaacactt tttccatcgt ctcgatatac agacgctcgc gagtaatttc
    24901 cggcgcggct ttatattccg gcagaagttt agcaaagcgc gccacttcac cctgagcttc
    24961 caggatggtc tgggccttgt acgcacgcgc ctcttcgagg atacgttgcg cctgaccgtt
    25021 cgcacgcggc tgaacttcgt tggtatacgc ttctgcttca cgaatatatt gctgttcgtt
    25081 ttcacgcgcg gcaatcgcat cgtcaaacgc cgcttttact tcttctggcg gacgagctgc
    25141 ctggaagttg acgtccagca gagtgatacc catgtcatac ggacgaatcg tctcttccag
    25201 ttcgcgctga gtatcgctac gaatcacggt acgaccttcc gtcagaatgc ggtccatggt
    25261 gtatttaccg ataaccccgc gtagggcgct gtcggtagcc tgacgcaggc tgtcatccgg
    25321 gctggtcacg ctatacagat atttttccgg attggtgacg cggtactgca cgttcatctc
    25381 aacgcgcact acgttctcgt ccgacgtcag catcacacca gaagcggcca gttcacgcac
    25441 ggcttccacg ttcaccggtt tgacttcgtc gataaacgtc ggtttccagt tcagacccgg
    25501 ctcaaccaga tggctgaatt taccaaagcg tgttaccacg ccacgttcgg cttctttaat
    25561 ggtatagaaa ccactggccg cccagataat gacaatcgct gccgctgcga tggtaacgac
    25621 acgaccgcca agctgcgggc gcgggccttg cgatgaactg ccaccgccag atccggtgcc
    25681 tttaccgccg cccagaccac cgagcttttt gctcagtttg cggaagatat catctaaatc
    25741 aggtggccct tgatcgcgac cgcctttgtt tccatttccc tcagagttgc cgccaggttt
    25801 gctgcttccc cacgggtcgc ggtcttgtcc gttattaccg ggctgattcc acgccatgtt
    25861 tgtgctccat atttgttatg cggtgatccc tgttgtcttt cgacgccaga ggatatgact
    25921 ccacgcttca gacgctacgc cgttagatca ggtaatcgat caacgccggt tcttgtttac
    25981 agaggcgacg ccagtcaacg atcggcatac gaacttgcag acttacgctg ccgtcctcct
    26041 ccatccactc tttttctatt gcctgaagct gataaaaacg acttctcaga cgcccttcct
    26101 gcggtggcag acgcaatgta tgctgcgcca cctcgccgga aagccgctcc gtcaaagcct
    26161 gaaaaagctg tggtatcccc gctccggtct gtgcggaaag ccagacacgg atcggtttgt
    26221 tctcttcgtc ccgatcaata cgcggttcga aatcttccag catatcgatc ttgttcatca
    26281 ccagcagggt tgggatctcg tgagcgtcga tctcttcaag aaccgtattc accgcttcga
    26341 tgttttcttg tacacgtaca tccgccgcat caatgacgtg cagcagcaat gtggcttgcc
    26401 gcgtctcttg taacgtggct ttaaatgccg ccaccagatc gtgcggcagg tggcgaataa
    26461 accctacggt atctgccaac acggtttcac cgacatccgc gacgtcaata cgccgcaacg
    26521 tcgggtcgag ggtggcaaac aactggtctg ccgcgtagac ccgcgcttcg gtgatgcgat
    26581 tgaaaagggt agatttaccg gcgttggtat atcccaccag cgaaacagta ggaacgtcgg
    26641 ctttgatacg cgattgccgc ccctgctcac gctgcttttc aactctttcc aggcgcgact
    26701 gtatctgcac gatgcgatta cgcaacaaac gacggtcggt ttcgagctgg gtttcacccg
    26761 gaccacgcaa accaatcccg cctttctgtc tttcaaggtg ggtccagcca cgcaccaggc
    26821 gcgtagccag atggcgcagc tgcgccagct caacctgcaa cttaccctca tgggtacgcg
    26881 cacgttgggc gaaaatatct aaaataaggc cggtgcggtc gatgacacga cactcgcaca
    26941 aacgctccag gttacgctct tgcgccgggc tcagggcatg gtcaaaaaga acgaccgaag
    27001 cacccgtcgc tttgacagct tccgcaattt caactgcttt accttcacct acaaaatact
    27061 ttgggtgcgg cgctttacgg ctaccggtaa tcacctgcaa tgcttcgaca ccggcggaag
    27121 agaccagaga ttcaaactcc tggagatctt ccatatcttt gtcttgcgta aaatagatgt
    27181 gtaccagtac cgcctgctca ccagcatcat aacggtcaaa caagcgtata accctctaaa
    27241 tagatcagcg gggaacgcag gatcgctggc tccccgtgta aaaaaaacag cccgaaacct
    27301 tattcggttt cttcgctgtc ctgttgcgcg gaagtattct gcgcgctgct accatgatgg
    27361 tagttactgc tggtaccgcc accggcgttg ttactgtgat gagaaaccgg gcgagacggg
    27421 acaacagtag aaatcgcgtg cttgtaaacc atctggctga ccgtgttttt caacaggatc
    27481 acgaactgat caaaagactc gatttgccct tgcagcttaa taccattcac caaataaata
    27541 gaaactggaa cacgttcccg acgcagtgcg ttcaggaacg gatcttgtaa agattgcccc
    27601 ttagccattc tctcttttcc ttatatgctt atttgtactt tgaacctttc gattctgaaa
    27661 aaattgcgca cgatacgtct caattgtaca cattcagcct gcgatagcac caacaacctg
    27721 taatacttcg tcacgcgcct gttctggttt ttcactatca agccagtgaa ccccttccca
    27781 accacgcagc caggttatct gccgcttcgc caactgtctc gtggcgcaaa cacctcgata
    27841 aaccatttca tcgtatgaga tttcgccttc aaggtaagac cacatctggc gataacccac
    27901 gcaacgaatg gaaggcaagt ccgtatgcaa atctcctcgg gcaaaaagcg cccggacttc
    27961 tgcttcaaaa cctgaagcca acatctgatg aaaacgctgc tcaattcgtt gatggagcag
    28021 ttcacggctc gccggggcga tggcgaactg atgcacctga tacggtagag cgtctcctga
    28081 cgtttgcgtc agttccgtta aagttttacc cgaaatgaaa aaaacttcca gtgcccggga
    28141 aagcctttgt ggatcatttg gatgaatcct tgctgccgca accggatcga tctcctgaag
    28201 ttgacgatgc aatgactccc aaccttgctc tgccgcctgt tgctcaattc tggcccgcac
    28261 ttccgggtct gccgacggta gcggcgacaa cccttccagc aatgccttga aatacaacat
    28321 cgtaccgccc actaacagtg ggatccgccc cgccgcggtg atatcggcca tttccgccag
    28381 cgcatcacgg cgaaaatcag cagccgagta agcctgcgac ggatcgcgaa tatccagcaa
    28441 tcggtgcggc gcggcgagta actcttcagc gttcggcttc gccgtcccga tatccatccc
    28501 tttgtaaata agggcagaat caacgcttat caactctact ggtaaaattt tacgcagctc
    28561 aatggctaac gccgttttac cggaggccgt cggccccatc aaaaaaatcg ccttaggcag
    28621 gctcgcctta ctgatatcac tcatctttca gggcttttat cgccggatgt aaatcaacag
    28681 attgtaacag accacccggc ggcgttttca caagttgcgg acataaccgt tccacgtccg
    28741 ccagcagagt tatggcctgc gccattgacc actgcgcatg ttcgctcatc agatttcgtg
    28801 caatccactg cgcaatattg ccaggttcga atacggactg cttcgccagg tagcctatca
    28861 gttcaggaat caagatttgt aaattttgtt ggcgtaaggg taaaggcacg gccctgatgg
    28921 tcacatgctg tgcatctgac tggaaatcaa tacccaattc cgccagggca gactgcgctt
    28981 tttctaatgc cgatttttct tcgccagaaa cttttagccg caacggaatc agcagcggct
    29041 gggcgcaaac tggcgcttca cccggcgtca gttgcgcctg acgcagccaa cgttctgcca
    29101 ctggcaaggc taaaagtgaa atgttgccgc cgcgctccag caacgcacag tcggaatgga
    29161 cgatagtcag tacccgacca aaactctgac tgttcgccgc aagtgcaggt tcctgcggtt
    29221 ctggcgcttt tggtttttgc atcggcgcgg gcgtttgcaa aagctggcga tacacttcac
    29281 cttgctgttt ctggtaacct ggctgcgcat tcggccaggg ggcagccgga cgactgcctg
    29341 aggcgggcgc aggagtgtaa cgcggagcta ctggctcacg aactgccggt tcagcaaaat
    29401 gattgcgccc cgccgccacg cggttttccg ggatggcgcg cggtgcaggt tgtggttcat
    29461 cgtccagcgg tagcggcgtt tccagttgct gttgtagcac gctcagcacg ccctgataga
    29521 taaaatcatg caccagacgc gactgatgga aacgcacttc gtgtttggca gggtgcacat
    29581 tgacgtccac ctgatgtggg tcgatctcca gatacagcac aaatgccggt tgctgatcgg
    29641 cccccagttt gtcttcacag gcctggcgga tcgcgtgatt gatcaggcga tcgcgcatca
    29701 tgcgaccatt cacatagcaa tactgaattt ccgccagtgc gggcgtggtg tgattcggat
    29761 ctgccaccca gccgcgcagc gtaagatcgc cgtgttgcca ttcaatcgcc agcgcttgtt
    29821 cgagaaaagc ggtaccgcaa atcgcgccta agcgccgttc tttttgcccg ccttccggca
    29881 ctgcgcggta ctgacgcaca attttaccgt tatgcgacag gttgatcgtg acgtcgaaac
    29941 gcgccagcgc aatgcggcgg atgatctcat cgatatggtt aaattcggtt ttctcagtgc
    30001 gcaggaattt gcgccgcgcc ggagtgttgt agaacagatc cagcacctcc agcgtcgttc
    30061 ccacaggatg cgccgccggt tttaccgtca cgtccatatc gcgcccttcg gcataggcct
    30121 gccaggcttc ctgctgttct gcggtacgtg aagtgagcgt caggcgggaa accgaactga
    30181 tactcgccag cgcctcaccg cgaaagccca ggctgataat ggcttcgaga tcgtccagag
    30241 aggcgatttt actggtggca tgacgagcca gcgccagcgc cagctcatct tttttgatac
    30301 cgcagccgtt atcacgaatg cggataagtt tcgccccacc gcgttcgata tcaatatcga
    30361 tacgcgtcgc acctgcatcg aggctgtttt ccaccagttc tttgactacc gacgcaggtc
    30421 gctcgaccac ctcacctgcg gcaatctggt tcgccagttg tggcggtaag acctgaattg
    30481 gcatcaatcg tccttagttt ggcagcgtgc gatctggcgt cgtcaccgta ctggcagttt
    30541 gtgccgctgt accctgcggc gcagattgca tcggatgcgc aaggaaataa ttacgcaggc
    30601 ctttgtaaat ggcttctgcc agctgttgtt ggtaatcgtc gctcgccagc aaacgttctt
    30661 cgctgttgtt gctgataaaa ccggtttcga ccagtactga tgggatatcc ggcgaacgca
    30721 gaacgccaag gctggcgtgt tctggtcgac gtttatgtat ttcgccaatg cgttgcaact
    30781 ggctgatcat actggtcgct acatcatacc ctacacgctg ggaatgaccg aactgtaaat
    30841 ccagcaccgc ctggcttaaa tagggatcag actgactgtt cgccagcaca tcgcccgctc
    30901 cgcccagcag ctccgactgt ttctcatgct gttccagcca gcttgccatc tcgctgtttg
    30961 cacgacggtt tgagagcacc catacggaag cgccagtcgc actgcggttc ggtgcggcat
    31021 cagcgtgaat cgacacgagg aaattggcgt tttgcttacg tgccacatcg ctgcgcccca
    31081 tcaccgagat aaagtaatcc ccgtcacggg ttaaaacgcc tttaaacatt ggatcgtcat
    31141 tgagcaaagt acgcaattta cgtgcgatgg cgatggtgac atttttctcc cgcgtaccgc
    31201 cggggccgat agcgccaggg tcctgaccgc cgtgtcctgc atcaatagcg atgataattt
    31261 tatcgccagt gttagccgtc gcacgcgctg ccggacgcgt taccgtatta ctgctgataa
    31321 cacctgtagt gcggttactt tccgttttaa acggattgcg cgccggttcg ctgacgcgcg
    31381 gtgcgacaac cgcaggcgtt tcaacgcgtt tcgcaaccac aggcggcgga ggaggcggtg
    31441 gcggcacatc ggcgttaatc gtaaagacga cagtgtaatt gctgccattc tgccgcttca
    31501 ccgcttcggt tttaccgttt tcggtaagat cgaccaccag ccgtagcgtt tgtgcatctt
    31561 taggcgttcc ggagcgaatc gccttcacca gattattgcc gctaaacaac aacggcagcc
    31621 cctgaattac ccccgtttgt ttgatatcga gcgccacggt gcgtttgctt tgatggctaa
    31681 acgcataatc aggatcgcca ataaaactca acgttatccg cgcctgttgg ttgccgttag
    31741 aaacctgaat atcagagagc gtcgcggcac ccacctgcgc gcacagcagc agcaacgtcg
    31801 ctaccaacca atttctgatg cgatacatca tcccgccacc tttcaaaggt taaccggcta
    31861 aacgcgccag caacaattca cccgcagagg aaaccgcact cacgcgcgcc tcacggcctt
    31921 gtgcctgata atcaatgtgt atttcgacat ccgggtcagg aagaacacct gtaccttgtt
    31981 gtggccactc caccaggcag atggcatcgt tggcaaaata atcgcggatc cccataaact
    32041 ccagctcctc gggatcggca aggcggtaca aatcaaagtg ataaaccatt aaattgtcga
    32101 gcgtataggg ttcgaccagc gtataagtgg ggcttttgac attaccctga tggcccaaag
    32161 cctgtaaaaa gccccgacta aaggtggttt tacctgcgcc taaatcgcca tacagataga
    32221 ttacggttgc gccatcgcag gctttcgcta cccgctcgcc caggtctaat gttgcctgct
    32281 catcagggag cggaattact cgattcatca tggtttttat cagtcacttc cgggttaaca
    32341 atacgctgta gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc aaaacgcgcc
    32401 gccagtacgt cagctgccgc accgtgcgcg acgcagcccg cacaggctgc atcatacggc
    32461 gacagttttt gcccaagcaa tgcgccaata ataccagaga gcacatcgcc catgccgcca
    32521 ctcgccatgc ctgcattccc ggcatcaata atgcctaaag cgtcaggatg ggcggcgacc
    32581 acggttccgg cacctttcag caccgctacg ccgccataac gttgtaccag acgtttagcg
    32641 caatgtaagc ggtcactttc aatttcagcg acggaacagc ctaacaaccg tgcggcctcg
    32701 ccaggatgcg gcgtgatcac gcgattgtga cgcttatcgg gattgattgc cagtaggttc
    32761 aatgcatcgg catcccacaa catcggtttg cgaaaattct caactttttg cagggctttt
    32821 ttcccccact cttgctggcc cagtccggga ccaatcacca gcacatcggc ccattccagg
    32881 ctttcggtaa gagagtccat agtcagttca tgcaccatca attctggtcg tgcagtcagt
    32941 agcggcgcaa tgttttcact gcgggtcaat actcgaacta aaccagcacc agcacgcagt
    33001 gccgcttccc ccgtcatacg aatagctccc gccgtgccgt gatcgccgcc aataatcacc
    33061 actctcccgt gatcgccttt atgcgatgtc gggcgacgcg gtttcagcca gtgagaaagt
    33121 tgttctgccg aaaaccgctg aattttcgtc tcctgacctg ccagccagct atccagcccc
    33181 agtgagtcaa aatgcagttg tccggtaaca tcccgcgctt ttccagtgag caagcctggt
    33241 ttcagcgcaa taaaagtgat ggtgtgatcg gcgttgatca ccgcgcctgg cgtagcgcca
    33301 gtttcagcca gcagcccgga agggatatca accgccacaa tcggcgcagg atgggtatta
    33361 gcgtggtcga ttaactggct aatggattcg cggggtgctt gctgcaagcc ggtaccgagc
    33421 agcgcatcaa caatcagatc taccgattcg ggccagacag tattcgaagc atggatctcg
    33481 cctcccgcgt ttaaccatgc ttcgcgtgac agcgcggcct cttccggcaa cggtttgtca
    33541 ctttcctggg ccagcagcgt gacctcaata ccgaccgctt tggccagtcg cgcgaccacg
    33601 tagccatcgc cgccgttatt accatgaccg cacagcacca gccagtggcg ggcgtcagga
    33661 taagccgtgc gacacacctg gaatgcggct tcgccagcgc gaagcatcag ctcatagagt
    33721 gtgagtccca gcgcatctgc cgcctcgcgt tctccgcggc ggatatcgtc ggcgtaccag
    33781 acggtgtgtg gtatacttac ggggtttttc ttcattgtat ggtccgtcat gtcagagccc
    33841 ctcgatctca atcagttagc gcaaaaaatt aaacagtggg ggctggaact gggctttcag
    33901 caggtaggta ttaccgatac cgatctcagc gcgtccgagc ccaaactgca agcatggctg
    33961 gacaaacaat accacggcga aatggactgg atggcgcgtc acggtatgct gcgtgctcgt
    34021 cctcacgaat tattgcccgg tacgctgcgc gtgatcagcg tgcggatgaa ttaccttcct
    34081 gctaacgccg catttgccag cacgctgaaa aatcccaaac tcggctatgt tagccgttat
    34141 gcgctgggcc gtgactatca caagcttctg cgcaaccgac tcaaaaagct gggcgagatg
    34201 attcagcaac attgtgtttc gctgaatttt agaccgtttg tcgattctgc gcctattctc
    34261 gagcgcccgt tagctgaaaa agcaggactt ggctggacag gtaagcactc acttatcctc
    34321 aatcgcgagg ccggttcatt cttcttttta ggcgaattgc tggtcgatat tcctctgccc
    34381 gtggatcaac cagtcgagga aggatgcggt aaatgcgtgg cctgtatgac gatttgcccg
    34441 accggagcca tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc ttatctcacc
    34501 atcgaactgg agggggcgat cccggaagag ttgcgaccgt taatgggaaa ccgtatttac
    34561 ggttgcgatg actgccagct tatctgcccg tggaatcgct attcgcaact cactacagaa
    34621 gacgatttca gcccgcgtaa gccgctacac gcaccggaac tcattgagtt attcgcctgg
    34681 agcgaagaga agtttttaaa agtcacggaa ggatcggcga ttcgccgtat aggtcacctg
    34741 cgctggctgc gtaatatcgc cgtggcatta ggcaatgccc cctgggatga aacgattctg
    34801 gcggcgctgg agagtcgcaa aggtgagcac ccacttcttg atgagcacat agcgtgggcg
    34861 attgcgcagc aaatagagag acgaaatgcg tgcatagtcg aagtgcaatt gccgaaaaaa
    34921 cagcgtctgg ttcgggtgat tgaaaaaggg ttaccgcgtg acgcctgatt catccacagc
    34981 ttgtgaataa aaataaaaac gcattgcaat tcaagacgaa taaaatcgtc aagtgatcga
    35041 ctcgacaaaa cgaaatctta atttttaact tatctatcaa tagattaaaa aaatactatt
    35101 caccgtgcga agtttctttg aaattaagat tacggcctga ggctgtggat aactctgttt
    35161 acaagagttt gtcagagaca acaaaaacca gcaataacgc atcgcgtcgc tgtggatatt
    35221 ttattgagag aagaatttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc
    35281 aaggtcgtgc tctaccaact gagctattcc cgcttgggtg gtctgtgctt tacagcactt
    35341 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    35401 ctctaccaac tgagctattc ccgcttaatc ttcgtctttc gaaccgctac tgccttggct
    35461 gccttcactc accttagtca cttacttttg taagctccca aggattcgtt cagttgccgc
    35521 cttgtcgcaa ttcgaaatac tgcgattggg tggtctgtgc tttgcagcac ttttcaaatt
    35581 ttggagcggg aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc
    35641 aactgagcta ttcccgcgta ctacttaatt ttgctttcgt aattttcgca tttctgcgtc
    35701 gttacgggag gcgcattata cgagaaatct ttccctctgc aaccccccta aaaaattttt
    35761 tttgagtttt tatctcaaat gctgaaatta tcagcaagac gttcaattta gcgtcaaaat
    35821 cacgccgccg caagtggatt ccggcgctta cgtgaccagg aaaaatttta cagcttgata
    35881 aaatgctcgc gatagtaagc cagttctgcc accgactcac ggatatcatc catcgcctga
    35941 tgcgtcccct gcttggtaaa accatcaaga atttccggct tccagcggcg cgccagctct
    36001 ttcagcgtgc tgacatcgag ataacggtag tggaagtagg cttccagctc cggcatgtat
    36061 ttaaacagga agcgacgatc ctggccgatg ctgttaccgc aaatcggcga ttttcccgca
    36121 ggcacccact gttttaagaa ttcgagcgtt gccagttcag cttcacgatc gcccattgtg
    36181 ctcgctttca cgcgctccac cagcccgctg gcggtatggg tgcgcacgtt ccagtcatcc
    36241 atcagcgcca gctgttcatc agactggtgt actgcaatgg tcggcccttc tgccagaata
    36301 ttcaggttgg catcggtcac cagcgtggca atctcaataa tgcgatcgcg ctcgggatcc
    36361 agaccggtca tctcaagatc gatccaaatc aggttgtttt cattggcact catgctattt
    36421 tccacccatt cacagtgact atattgatcc acaattgcgt gtatcataga tgttttgccc
    36481 atcaggggcg accaggagtc tgtacgattg agtaaaaata aactctccaa aggccagcag
    36541 cgccgcgtga acgccaatca ccagcgtcgt cttaaaacgt ctaaggagaa gcccgactac
    36601 gacgacaatc tgtttggcga gcctgatgaa ggtatcgtca tcagccgctt tggtatgcac
    36661 gctgatgtgg aatccgccga tggcgacgtt caccgctgca atattcgccg taccatccgt
    36721 tcgctggtga ccggcgaccg cgtagtctgg cgtccgggta aaccggcggc ggaaggcgta
    36781 aatgtcaaag ggatcgtgga agcggtgcat gagcgcacct cggtgttgac gcgcccggac
    36841 ttctacgacg gcgtgaaacc tattgccgcc aacatcgacc agattgttat tgtctccgcc
    36901 attttgccgg agctgtcgct caatattatc gaccgttacc tggtggcctg cgaaaccttg
    36961 ctgattgagc cgattattgt gctcaacaag atagacctgc tggacgacga aggcatggag
    37021 ttcgtcaacg aacagatgga tatctaccgc aacattggtt atcgcgtatt gatggtttcc
    37081 agtcatactc aggatgggct gaaaccgctg gaagaggcgt tgaccggacg catcagcatt
    37141 tttgccgggc agtctggcgt tggcaaatcc agcctgctta acacgttgct ggggctgcaa
    37201 aaagagatcc tgaccaacga tgtttctgac aactcgggtc tcggtcagca caccacgacc
    37261 gccgctcggc tgtatcactt cccgcacggc ggtgatgtga ttgactcccc tggtgtgcgt
    37321 gagttcggcc tctggcacct ggagccggaa caaatcactc agggctttgt cgaattccat
    37381 gactacttag gtctgtgtaa atatcgcgat tgcaaacacg ataccgatcc gggctgcgct
    37441 atccgggaag cggttgagga agggaaaatc gcggaaaccc gtttcgaaaa ctatcaccgt
    37501 attctggaaa gcatggcgca ggtaaaaacg cgtaaaaact tttctgatac ggatgactga
    37561 caattaagct aaccgtcgtt agaatcgtcc cctttttttc aggatccggc atgtaggccg
    37621 gatcaggaac gacaaaacaa tggcctggag gctaccttgt taaattcatt taaactttcg
    37681 ctacagtaca ttctgccgaa actatggctt actcgcctgg cgggttgggg cgcaagcaag
    37741 cgggcaggat ggctgacaaa actggttatc gatctgttcg ttaaatacta caaggtcgac
    37801 atgaaagagg cgcaaaagcc ggataccgcc agctaccgca cctttaacga attctttgtc
    37861 cgtccgctgc gtgacgaagt acgcccaatc gataccgatc cgaatgtact ggttatgcct
    37921 gccgatggcg ttatcagcca gttgggtaaa atcgaagaag ataaaatcct gcaagccaaa
    37981 ggccacaact acagcctcga agccctgctg gcaggcaact atctgatggc ggacctgttc
    38041 cgcaacggta cgtttgtgac cacttacctc tccccacgtg actaccaccg cgtacacatg
    38101 ccgtgcaacg gtattctgcg tgaaatgatc tacgtgccgg gcgatctctt ctccgttaac
    38161 catctcaccg ctcagaacgt gccgaatctg tttgcccgta acgaacgcgt gatctgcctg
    38221 ttcgataccg aatttggccc gatggcgcag attctggtcg gtgcgacgat tgtaggcagc
    38281 attgagacgg tctgggcggg caccattacg ccgccgcgcg aaggcatcat caagcgctgg
    38341 acctggcctg ccggggaaaa cgacggttct gtggcactgc tgaaaggcca ggaaatgggt
    38401 cgctttaaac tcggttccac cgttatcaac ctgtttgcac cgggtaaagt gaatctggtt
    38461 gagcaactgg aaagcctgtc tgttacgaaa attggccagc cgctggcagt atctaccgaa
    38521 acctttgtta cgccagacgc tgaacccgcc ccgctttctg ctgaagagat cgaagcagaa
    38581 cacgacgcca gcccattggt tgacgataaa aaagaccagg tctaatccat caaaggaaac
    38641 gctgacgtgc gcctgattat cacttttctg atggcctggt gcctcagttg gggggcgtac
    38701 gccgcgacgg cccccgatag caaacaaatc tcacaagaac tggagcaggc aaaagcggcg
    38761 aaacccgcgc aaccggaagt cgtagaggcg ctccagtctg ccttaaatgc gcttgaggaa
    38821 cggaaaggtt cccttgagcg catcaagcaa tatcaagaag tcattgataa ttatccgaaa
    38881 ctctccgcta ctctgcgcgc gcaattaaac aacatgcgtg acgagccgcg cagcgtgtca
    38941 ccgggaatgt ctaccgacgc gctgaatcag gaaattctcc aggtcagcag ccagttgctg
    39001 gataaaagcc gtcaggccca gcaagagcag gaacgcgccc gcgagattgc cgattcgctg
    39061 aatcaactgc cgcaacagca aaccgatgcc cgccgtcagt taaatgaaat cgagcgccgc
    39121 ctggggacgc ttaccggcaa ttccccgctc aatcaggcac aaaatttcgc gttgcagtct
    39181 gactccgcac gcctgaaagc gctcgtcgat gaactggaac tggcgcagct gtccgccaat
    39241 aaccgccagg aattagcgcg cttacgctca gagctggcgg aaaaagagag ccagcaactg
    39301 gatgcgtatt tgcaggcctt acgtaatcag ttgaacagcc aacgtcagct tgaggcggag
    39361 cgggcgctgg aaagtaccga attgctggca gaaaacagcg ccgatttgcc gaaagacatc
    39421 gtcgcgcaat tcaaaattaa ccgcgaacta tcagcggcgc tgaatcaaca ggcgcagcgg
    39481 atggatctcg ttgcctcgca acagcgtcag gctgccagcc agacgttaca ggtccggcaa
    39541 gcgttgaata cgctgcgtga acagtcgcaa tggctgggat cgtccaatct gctcggcgaa
    39601 gcgttgcggg cgcaggtggc acggctgccg gaaatgccaa aaccacaaca gcttgatacc
    39661 gaaatggcgc agttgcgtgt gcaacggtta cgttatgagg atctgcttaa taaacagccg
    39721 cagctacggc aaatccatca ggccgacggt cagccgctga ccgccgagca aaaccgtatt
    39781 ctggaagcac agctacgcac tcagcgtgag ttgctgaact cattgttgca gggtggagac
    39841 acgctactac tggaactgac caagctgaaa gtctccaacg ggcaactgga ggatgcgctg
    39901 aaagaggtga atgaagcgac gcaccgctat ctgttctgga cctctgacgt gcgcccgatg
    39961 accatcgcct ggccgctgga aatcgcccag gatctgcgtc gtctcatttc gctggacacc
    40021 ttcagtcagt tgggcaaagc cagtgtgatg atgctgacca gtaaagagac aattttaccg
    40081 ctgtttggcg cgttgattct ggtcggttgc agtatttact cacgccgcta tttcacccgt
    40141 tttcttgaac gttcggcggc gaaagtcggc aaagtgactc aggatcactt ctggctgacg
    40201 ttacgcactc ttttctggtc gattctcgtc gcgtcaccgc taccggtgct gtggatgacg
    40261 ctgggttacg gcttgcgcga ggcgtggcct tatccgctgg cggtcgcgat tggtgatggc
    40321 gtaacggcca ccgtgccgct gctgtgggta gtgatgattt gcgccacctt tgcccgcccg
    40381 aacggcttgt ttatcgctca ttttggctgg ccgcgcgaac gggtttcccg tgggatgcgc
    40441 tactacctga tgagcatcgg gcttattgtg ccgctgatta tggcgctgat gatgttcgat
    40501 aacctcgacg accgtgaatt ctccggttcg ctgggacggc tttgctttat cctcatttgc
    40561 ggtgcgctgg cggtggtcac tctcagtctg aaaaaggccg ggatcccgct gtatctcaac
    40621 aaagaaggca gcggtgacaa cattaccaac catatgctgt ggaacatgat gattggcgca
    40681 ccgctggttg ccattctggc gtcggcggtg ggttatctgg caacggcgca ggcgctgtta
    40741 gcgaggcttg aaacctcggt tgccatctgg tttttgctac tggtggttta tcacgttatc
    40801 cgccgctgga tgctgatcca acgccgcagg ctggcgtttg accgggcgaa gcatcgccgg
    40861 gcagagatgt tagcgcaacg tgcgcgtggc gaagaggaag cacatcatca cagtagcccg
    40921 gaaggggcaa ttgaagtcga tgaaagcgaa gtcgatctcg atgccatcag tgcgcagtcc
    40981 ttgcggctgg tgcgctcaat tttgatgttg atcgccctgc tttcggtcat tgtgctgtgg
    41041 tcagaaatcc attccgcttt cggcttcctc gaaaatattt cgctgtggga tgtcacctcc
    41101 acggtacagg gcgtagaaag tctggagccg attaccctcg gtgcggtgct gattgccatt
    41161 ctggtgctta tcatcaccac gcagctggtg cgcaacctgc ccgcgctgct ggaactggcg
    41221 attttgcagc acctggattt aacgccgggt acaggttacg ccatcaccac catcaccaaa
    41281 tatctgctga tgctgattgg cgggctggtc ggcttctcaa tgattggtat tgagtggtcg
    41341 aaattgcagt ggctggtcgc cgcgctcggt gttggtctcg gttttggttt gcaggaaatt
    41401 ttcgccaact ttatctctgg cctgatcatc ctgttcgaaa aaccgattcg cattggcgat
    41461 acggtaacga tccgcgatct tactggtagc gtgacgaaaa ttaacacccg cgccaccacc
    41521 atcagcgact gggaccgtaa agagataatc gtgccgaaca aggcgtttat taccgagcag
    41581 tttatcaact ggtcgctctc tgactcggtc acgcgcgtgg tgttgacgat tccggcccct
    41641 gccgatgcca acagtgaaga agtgacggaa atcctgctca ccgcagcgcg tcgctgctcg
    41701 ctggtgatcg acaacccggc accggaagtc ttcctggtgg atctgcaaca ggggattcag
    41761 attttcgagc tgcgtattta cgccgctgag atgggtcacc gtatgccgct acgccatgag
    41821 atccaccagc taattctggc aggcttccat gcccacggta tcgatatgcc attcccgccc
    41881 ttccagatgc gtctggaaag cctgaatggt aaacaaacgg ggagaacatt aacttctgcg
    41941 ggcaaaggtc gtcaggcggg aagtttgtaa ggttttgtgc cggatgaacc catccggctc
    42001 acaaacaaaa ctgatagcgc agaaaaaact gccacagcca gtagccgaaa atagccagcg
    42061 cggtggcgta cataaaccag ccgcgttttt ttcgccacaa agcgaaccag agaaaaggga
    42121 aaaagagcgg taacgtcatt atcacaccga catcgcgagt atcgtcatct tcgagataca
    42181 tcaaaacatc acagacgttg ttaatcccgc gtccatcaat catccactct ttgtccagcg
    42241 cggaggtaat ggttatccag aggaacgcca caatgaacca gatgcagcac aagacaaaca
    42301 tgcgcgcact catgagataa cccgtagttt aatttcgcta tagcacatac gaaatcccac
    42361 taattccctg tcattgcgcc ctatcgtcag acagacgata gggcatcggg ttattccacc
    42421 acccactgcg gattttttgc cactctgctg caatagttct ggtaaatcgc catcgctaac
    42481 agcgagaaga agacagggcc gccaatcatc cacaatgtgc tgtcccagtc tccggcttca
    42541 accacaggtt gaatgatggt gaagacgttc gcaaatgtca ccaccagaac gacaaccacc
    42601 gtcgcaatca ttgccgacat acgcgttttg aaaatcacaa atggtctgtc gagatcctga
    42661 cgtgctttaa agaatgggaa agccagcgcg aggaacaggt aaggaagcgt catagacacg
    42721 ttcgccatca gcgtcagctt gttaaagaac gccgatgcgg taccgccacc aaacgaaacc
    42781 agcaggatga agacagtaac caacccgcac tgcatccaca tggcgataga cggcatcccc
    42841 atcgcattca ggcgcgtcat cggttccggc cacaacgctt tcggcgtccc ctggatgatg
    42901 gctttcagcg gtgaatagca aagcgtaaag aacgcaccgg tataagcgag gaacatcgaa
    42961 agtccggtaa tacgcgcaaa ccatacgccc agcgacaatg acgcttctgg tgacaaatgc
    43021 agtgcgttac ccagcgtcac cccgaggctc ttcatcagca cataggtaat attgccgagg
    43081 ttaacggaac cattacttaa tacctgctgc cagtttgtgc tgacgcccca taaaaatatt
    43141 gccagcgaat aaccgattga aataacaata gcggcaaaaa caataccttt ggcaaagttc
    43201 ttttctggat tttccgtttt atcgaccaga ccaccaaccg cttcaattcc gccataggca
    43261 aaaatagcaa ataccacaaa cgatagcatt gccagaccgg actgataacc tgggttcggt
    43321 gatgcaagga aattaatatc ctgcgcgaaa tgcccaccat ttaataacaa aatagtaatg
    43381 cttactaaca gcaatactaa attcagacac attactgcaa taccgcccac cgcagtaatg
    43441 cgggcaattt tattaatccc ctttgaagca acgacggtga ccagaatcat ccatgccact
    43501 gccagcagac caaccacctg cgtaggctcc agtccggtaa tacgccagtg ctgggtcatg
    43561 tcgctaccat agaggaatgt tgagaacgga acccaaactt tcgcggaggt actcaccatc
    43621 cagatgatat aagaggaaaa ccacataaat gtaccaatga aggcaaaacg tggtccgaca
    43681 ctattattca tccaggaata gataccacct tcttctttgc gataagcagc tcccatttca
    43741 gccatcatta aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc
    43801 gcactataac ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta
    43861 aagatcatca atatgagtcc tatcagactc acctttttta tcgtgtgagg catgaaacca
    43921 tccttcattt cagcgcgtgt ataaaacgcg cccactgaac tgcactgccc aacattgagc
    43981 gggcagcgta aaaaccaata cagaattgat acctggatga tatcaaatat ccagtcttaa
    44041 attgtggcct ttagcggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat
    44101 tcttcgcggt taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga
    44161 cttcagccag tgtctccgcg cccagcgcca acatcaccag acgatcaacg cctaatgcca
    44221 cgccggaaca gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt
    44281 gctgcggcaa accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct
    44341 cacgggcatc cgtcaattca tggaaaccat tcgccagctc aataccttta taataaacct
    44401 caaagcgttc agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg
    44461 ccggaaagtg gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg
    44521 taaacagcag ttgtagcagc gtgtcgcggt cttcttcggt atcggcaacg ttgctcaaat
    44581 ccagtttcgc tgccacttcc cgcagttgcg ttttgtcagc agagagtggg tcaatttcca
    44641 gataacgcaa gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct
    44701 gttgtaagag atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc
    44761 actcaagcat agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc
    44821 ggcacagctg gaataccggc ccacaaccag caaccagcag gcgtttcata tggtattccg
    44881 ggctggtcat taaccagaga ttcatcccct gcgaatgccc ggggcctacg aaacgtgtct
    44941 caaacgggac caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct
    45001 ccagcactcc acgatcggca aagaaacgac ggatctccgc cataatcgtc gcgcgtttta
    45061 ataagttagg aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc
    45121 cagtttttga caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca
    45181 ggaatcaaac aagggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa
    45241 attaatcatc ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa
    45301 atttcagact tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt
    45361 tcgcagaccg taactttcag gcacttaccc tgaagtacgt ggctgtggga taaaaacaat
    45421 ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc
    45481 gggattacgt gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat
    45541 ctcaaaagta tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt
    45601 cgcgcaggat catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg
    45661 gttgtgtgag caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca
    45721 actggaactg tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg
    45781 cttcggcggc atgaaaatcg agcgtacctg gttcgccgcc gataagaccg gcttccatat
    45841 gctgcacacg ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca
    45901 tttcgtgctg gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat
    45961 gatggaaggc acgctggtgc agatccgtgc taacgcggtc gttatggcta ccggcggtgc
    46021 gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat
    46081 ggcgctaagc cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg
    46141 tctgccaggt tccggtatcc tgatgaccga aggctgccgc ggtgaaggcg gtattctggt
    46201 caacaaaaat ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg
    46261 cgagccgaaa aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg
    46321 gcacgaatgg cgtaaaggca acaccatctc cacgcctcgt ggcgatgtgg tttatctcga
    46381 cctgcgtcac ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc
    46441 gaaagcgtac gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca
    46501 ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt
    46561 cgccgtgggt gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc
    46621 cctggcggaa ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc
    46681 aactgccggt aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg
    46741 tctgaaagat ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat
    46801 gggcctggct atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac
    46861 catcgacaag ctggctgagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc
    46921 cagcgtgttc aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc
    46981 tgaatgtatg gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct
    47041 ggacgaaggt tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg
    47101 cgatgctgat ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc
    47161 agctaaacgt gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa
    47221 gaaggagaag gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc
    47281 cggaagtcga taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct
    47341 cattactgga tgcgctgggc tacatcaaag acaacctggc accggacctg agctaccgct
    47401 ggtcctgccg tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac
    47461 tggcatgtaa aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta
    47521 acttcccgat tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag
    47581 cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga
    47641 ccccggcgca gatggccaag tatcaccagt tttccggttg catcaactgt ggtctgtgct
    47701 acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc
    47761 tggcgcatcg ttacaacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt
    47821 tgaacagcca gaacggcgta tggagctgta ctttcgtggg ctactgctcc gaagtctgcc
    47881 cgaaacacgt cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagatt
    47941 ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg
    48001 tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg
    48061 ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg
    48121 tttgccctga aaaatggccc ggaaagctgg gcgggattcg tcgacttttt acaaaacccg
    48181 gttattgtga tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg
    48241 tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag
    48301 ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt
    48361 gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc
    48421 ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat
    48481 gatcctgctg gtgggtattc tgctgccgct ggggctgttt ccgggtgatg cactgagcta
    48541 cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat
    48601 cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat
    48661 ccacgtacct gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac
    48721 gctgattggt gtcgttacaa tctaacgcat
//
LOCUS       DAOTRK010000014        47200 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.014, whole genome shotgun sequence.
ACCESSION   DAOTRK010000014 DAOTRK010000000
VERSION     DAOTRK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.014"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..429)
                     /locus_tag="QIE41_000917"
     CDS             complement(<1..429)
                     /locus_tag="QIE41_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529832.1"
                     /translation="MPNTSIHLSPCNILQNNKLQPTEIYKESQQTAKLEFFCDEFLKI
                     SQSRYGLSTSADSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYEPYTLTFTRGENNEGILSVESKEGFISQRTINLNE"
     gene            complement(895..1773)
                     /locus_tag="QIE41_000918"
                     /pseudo
     CDS             complement(895..1773)
                     /locus_tag="QIE41_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1925..3115
                     /locus_tag="QIE41_000919"
     CDS             1925..3115
                     /locus_tag="QIE41_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529833.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLLVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNLAFWLCTGVAAVGIILSLIMTVPPVISHADGSTPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLVFFTVGLVVLGYTQGSIMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            3196..3846
                     /gene="inaA"
                     /locus_tag="QIE41_000920"
     CDS             3196..3846
                     /gene="inaA"
                     /locus_tag="QIE41_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDS3529834.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(3900..4154)
                     /gene="yfaE"
                     /locus_tag="QIE41_000921"
     CDS             complement(3900..4154)
                     /gene="yfaE"
                     /locus_tag="QIE41_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDS3529835.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4154..5284)
                     /gene="nrdB"
                     /locus_tag="QIE41_000922"
     CDS             complement(4154..5284)
                     /gene="nrdB"
                     /locus_tag="QIE41_000922"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDS3529836.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(5373..7658)
                     /gene="nrdA"
                     /locus_tag="QIE41_000923"
     CDS             complement(5373..7658)
                     /gene="nrdA"
                     /locus_tag="QIE41_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDS3529837.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            8354..12082
                     /gene="yfaL"
                     /locus_tag="QIE41_000924"
     CDS             8354..12082
                     /gene="yfaL"
                     /locus_tag="QIE41_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDS3529838.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVIDSCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMNNNASGGAVFLQQGAEFSLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSDNVAGGYGGAIYSSGTNDTGVVDLSVTNA
                     VFRNNIANDGKGGAIYTINNDVYLSEVIFDNNQAYTSTSYSDGDGGAIDVTDNNSNIT
                     HPSGFTIINNTAFTNNSAEGYGGAIYTNSVTAPYLIDISVDDSYSQNNGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGVITKTGSG
                     DLVLNANNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLTLEGDAADLAALQDDPQSIVLNGGVLDLSDFATWQS
                     GTSYNDGLEVSGSGGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVIDAGDGLVSLANN
                     NSYLGTTQIASGTLVVSDNSQLGNTDENRQLIFTDSQQQSEMEITADVDTRSEAAGHG
                     RDIEMRADGEVAVDAGVDTQWGGLMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFKTGADQDIQSIDVTSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVVIN
                     PITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEATPPSPPDPDPTPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQAFVMERHDHA
                     GGDGQTLNLRIIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGADGEWMLGAVGGY
                     SDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFNNDVSE
                     HEDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQAQVIYQGVQQDDFTAANHARVSQS
                     QGDDIQTRLGLHSEWRTAVHVIPSLDLNYYHDPHATEIEEDGSTISDDAVKQRGEIKV
                     GVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(12143..12865)
                     /locus_tag="QIE41_000925"
     CDS             complement(12143..12865)
                     /locus_tag="QIE41_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDS3529839.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            13012..15639
                     /gene="gyrA"
                     /locus_tag="QIE41_000926"
     CDS             13012..15639
                     /gene="gyrA"
                     /locus_tag="QIE41_000926"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDS3529840.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            15788..17476
                     /locus_tag="QIE41_000927"
     CDS             15788..17476
                     /locus_tag="QIE41_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDS3529841.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKTQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPDAAWQWLPITWQPL"
     gene            17473..18096
                     /locus_tag="QIE41_000928"
     CDS             17473..18096
                     /locus_tag="QIE41_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDS3529842.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFSANEALKVHDSKWLKSNGIASQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            18120..22634
                     /locus_tag="QIE41_000929"
     CDS             18120..22634
                     /locus_tag="QIE41_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDS3529843.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNSQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTINVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKDFKTGEAVSGKLQLLYPDGEPVK
                     DASVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSDAVSDDKGNVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTSAQYSNSGESVVFRYAALESSKQVPVK
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSLLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALILPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPMTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            22635..24284
                     /locus_tag="QIE41_000930"
     CDS             22635..24284
                     /locus_tag="QIE41_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDS3529844.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GSPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFANGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     SCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            24289..25065
                     /locus_tag="QIE41_000931"
     CDS             24289..25065
                     /locus_tag="QIE41_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDS3529845.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNSVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITEEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(25139..26323)
                     /gene="atoB"
                     /locus_tag="QIE41_000932"
     CDS             complement(25139..26323)
                     /gene="atoB"
                     /locus_tag="QIE41_000932"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDS3529846.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERARID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPAFMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(26354..27676)
                     /locus_tag="QIE41_000933"
     CDS             complement(26354..27676)
                     /locus_tag="QIE41_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDS3529847.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(27673..28323)
                     /gene="atoA"
                     /locus_tag="QIE41_000934"
     CDS             complement(27673..28323)
                     /gene="atoA"
                     /locus_tag="QIE41_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HDS3529848.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(28323..28985)
                     /gene="atoD"
                     /locus_tag="QIE41_000935"
     CDS             complement(28323..28985)
                     /gene="atoD"
                     /locus_tag="QIE41_000935"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDS3529849.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(29181..30566)
                     /gene="atoC"
                     /locus_tag="QIE41_000936"
     CDS             complement(29181..30566)
                     /gene="atoC"
                     /locus_tag="QIE41_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDS3529850.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(30563..32389)
                     /gene="atoS"
                     /locus_tag="QIE41_000937"
     CDS             complement(30563..32389)
                     /gene="atoS"
                     /locus_tag="QIE41_000937"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDS3529851.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELNPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            32556..35405
                     /gene="rcsC"
                     /locus_tag="QIE41_000938"
     CDS             32556..35405
                     /gene="rcsC"
                     /locus_tag="QIE41_000938"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDS3529852.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRTDGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(35491..36141)
                     /gene="rcsB"
                     /locus_tag="QIE41_000939"
     CDS             complement(35491..36141)
                     /gene="rcsB"
                     /locus_tag="QIE41_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDS3529853.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(36158..38830)
                     /gene="rcsD"
                     /locus_tag="QIE41_000940"
     CDS             complement(36158..38830)
                     /gene="rcsD"
                     /locus_tag="QIE41_000940"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDS3529854.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            39569..40663
                     /locus_tag="QIE41_000941"
     CDS             39569..40663
                     /locus_tag="QIE41_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDS3529855.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLYGKVDGLHYF
                     SDNDSKDGDKTYMRLGFKGETQVTDQLTGYGQWEYQIQGNEPESDNSSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYDSDNFMQQRGNGFATYRNTDFFGL
                     VDGLDFAVQYQGKNGSAHGEGMTTNGRDDVFEQNGDGVGGSITYNYEGFGIGAAVSSS
                     KRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDY
                     KINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            40775..41830
                     /gene="apbE"
                     /locus_tag="QIE41_000942"
     CDS             40775..41830
                     /gene="apbE"
                     /locus_tag="QIE41_000942"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDS3529856.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKTQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSLWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LSVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            41904..42968
                     /gene="ada"
                     /locus_tag="QIE41_000943"
     CDS             41904..42968
                     /gene="ada"
                     /locus_tag="QIE41_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDS3529857.1"
                     /translation="MKNATRLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYTDASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRDSLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            42968..43618
                     /gene="alkB"
                     /locus_tag="QIE41_000944"
     CDS             42968..43618
                     /gene="alkB"
                     /locus_tag="QIE41_000944"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDS3529858.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            43694..45337
                     /gene="yojI"
                     /locus_tag="QIE41_000945"
     CDS             43694..45337
                     /gene="yojI"
                     /locus_tag="QIE41_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDS3529859.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGNPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLIEKWLVQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGLLSELTGEERDAASRDAVARTA"
     gene            45555..>47200
                     /gene="mqo"
                     /locus_tag="QIE41_000946"
     CDS             45555..>47200
                     /gene="mqo"
                     /locus_tag="QIE41_000946"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDS3529860.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT        11544 a        11422 c        12560 g        11674 t
ORIGIN      
        1 ttcatttaag tttattgttc tttgtgaaat aaacccttct ttgctttcga cactcaaaat
       61 accttcgttg ttttcacctc gggtaaaggt aagggtatag ggttcataat ccgctgcaaa
      121 ttcattttta tacgttatga catgttcgac aaaatgtctt attggtacga aaccataact
      181 gtgaacgtca attttttggg tcttgatact atcaattgca tctgaggctt tcgtgaaaaa
      241 agccaataaa ttagctatgc tgtctgctga agttgataac ccgtatctac tttgagatat
      301 ttttagaaac tcatcgcaaa aaaactcaag tttcgcggtt tgttgtgatt ctttgtaaat
      361 ttctgttggt tgcaatttat tattttgtaa aatattacag ggagataagt gaatagatgt
      421 atttggcata aatgattcct tgtattgtga gttaacaaga tacaatctaa tctttgcaaa
      481 atatattcac tatcaaattt agctcatttt attacgaaga cctttacgca tggtagaaag
      541 tatcattatt gttaataaat acggtaataa gtaacaaaca gatattgata acttccctga
      601 aaagttacta ggtatcccac ttgaagattg agaatggctc cagagtaaac tgtgtactgt
      661 tcgccggagt gtcattactc tcaacgatcg gttttgacag tgagtcgggc gcttcatacc
      721 gatttctacc tggagtcatt gtcacttctc ccaactggat aaatatatat cgccaattca
      781 caaagtgggc caaaatcagg tgaaaacgaa ttattacgcc tggtaaaggc tatagtacaa
      841 ctaagtgtgg cgaatcaggc agtcttgcat ttgtcatcaa tctcatgtct tacatcaaat
      901 ttttccactc accactaatt gttctgtgca catttgtagt aaaaacagtg gactgccttc
      961 taccggcttt tctttagcca gcaacgaaat ctgtcgatag aaggttggtt cgagaggaac
     1021 ggaacacaat tcatctgcaa tggttttcag cgttaactct ggtaataagg cgatgcctaa
     1081 tccctgtcga ataaaactga tcgctgtatc cgggtgatta aattcatatt ttatctgtgg
     1141 ggtaatattc ttttctttaa atagtgccat aatgcttaat tcatagcgtc ctttactaat
     1201 aattaatggt tcacccatta actcttccac tgtaatagtg ctgttttttg ccagagcatg
     1261 gtcgggcggt aacactacgg tgaatttatc ctgataaatg ggcacggagt acattccgct
     1321 caccggaaaa tgaacaaaac cggcatcaat ggattcattc tgaagcgaat caataatcgc
     1381 tgtgctattt tcctcaaaag gaataatttt tatattcgga tgatgaattt caaagtagcg
     1441 aatcacaacc ggtaagatac aggcgcagac gctggggaaa caaccaagtc tcagcgttct
     1501 ttgagggttc tgcttttcct gctcggctat ctcttttact gcgttaactt cccgattaat
     1561 ggcgcgcaga tgttgcacaa tgcgatgacc tgctgtggtc agttgtatag ctttacggcg
     1621 atcgcgtaac agtatattca caccaagttc ttgttcgagg ctagcaattg cctgacttac
     1681 cgccgactgc gtcatgaact atcgcttgct ggcttcagta aagccaccgt aatccagcac
     1741 ggcgagtaaa gagtttattt gggtaagagt cattagagtt tgctaatcaa taatattaaa
     1801 acgattaatt tctctaatag catgcagcca tttatacttc tgttctgcaa agtacatcac
     1861 aatttgacgc aaccgcagta acgggagtgt tgtctgcgtg cgtcatcctg acaggcggta
     1921 gggtatgaaa gagaagttat ggacgaagga tttttgggca ataaccatca tcagctttat
     1981 tattttcttc gtcttttatg ttttactaac attgttgcca atttatatct ctgaccgctt
     2041 gcatgcctct cctgataaag ccggtttatt ggtgacttta tttttaattg ccgccattgt
     2101 tattcgccca tttgccgggc aatgggtggg taaatactcg aataaaacta ttctggtgct
     2161 ctcttctctg gcctttttat tggtcacagc gttgtatcct ttttgccact caattgaatc
     2221 actgcttttt attcgggtgc ttcatggtat taccttcggg gttatcacta cggtaaaagg
     2281 aacgatttcc gcgcggctga tcccggcctc ccgacgtggg gagggcatca gttttttctc
     2341 tctggcaatg gggctggcaa tggtggtcgg gccgtggatt ggtctgaata tggcgcgctg
     2401 ggaagccttt aatctggctt tctggctatg caccggcgtg gcagcggtgg ggattattct
     2461 gtcgctgatt atgacagtgc cgccggttat cagccatgct gatggttcaa cgccgaaaat
     2521 gggcttcgcc gccatgttcg atcgcgcggc gttgccattt gcgatggtca ccttttttat
     2581 gaccttttcg tatgccgggg tttctgcttt tctggcgctt tatgcacgcg aacttaacct
     2641 gatgtcggca gccagtaatt tcctgctttg ctacgctatc ttcctgatga tctgccgtac
     2701 ctttaccggc aatgtctgcg acaaaaaagg accgaaatat gtggtttacc cttgccttgt
     2761 gttctttacg gtaggactgg tggttctcgg ctacacccag ggaagcataa tgatggttgt
     2821 ttctggcgca ttgattggta tcgggtatgg ttccgtgacg ccagtttttc agacgcagat
     2881 tatcagttca gtggaaccgc ataaaatcgg tgtcgcaaac tccctcttct tcaatgcgat
     2941 ggatgcaggc ctggcgctgg gagcctgtgt gatggggatg atggttgcac atactggcta
     3001 ccgaatgatt tatctgctgg gtgcactatt agtggtagtg gctggtggag tctatgcgct
     3061 gcaaatgaag ggtaaaagcg gtgttgcgct agtagtggca aaagaaattc attaatacga
     3121 cacgtttcat taagattttt cgcaggtaac cacctatagt cattgtcgtt aataaaaaat
     3181 gacaggcgat agggtatggc agtttcagca aagcacgacg aatttaatca ctggtgggca
     3241 acagagggcg actgggttga agagcctaac tatcgccgca atggtatgag cggggtgcaa
     3301 tgcgtcgagc gcaacggcaa aaagctatat gtaaagcgca tgacgcatca tctgtttcat
     3361 tccgtacgtt atccgttcgg ccgaccaacg attgtccgtg aggttgcggt aattaaagaa
     3421 ctggaacggg ccggtgtcat cgtgccgaag atcgtttttg gtgaagcggt gaaaattgag
     3481 ggggaatggc gcgcgttgct ggtgactgaa gatatggcgg ggttcatcag cattgctgac
     3541 tggtatgccc agcatgcagt aacgccctat tctgacgaag tacggcaagc catgttgaaa
     3601 gcagtggcgc tggcgtttaa gaaaatgcat agcgttaatc gtcagcatgg ctgttgctat
     3661 gttcgccata tctatgtgaa aacagaaggt aaggcagaag ctggatttct tgatctggag
     3721 aaaagtcgtc gccgcttgcg tcgcgataaa gcgattaatc atgacttccg ccagctggaa
     3781 aaatatctgg agccgatccc caaagcggac tgggagcagg tgaaagcgta ttactacgcc
     3841 atgtaatcgt tgtaggccag ataatacgcg taagcgtcgt atctggcgct agtacccgat
     3901 cacaactcga tttcaatatc gccttttgcc cggcaacaac agggcaaaat ttcccccggc
     3961 tgaataaagg ctaacggttc ggcaatccag tccacctggc ctgcaacaag gcgcgtgcga
     4021 caggaaccgc agtaaccttc gcggcactgg tactcaaccg ccacattgtg ggattccagc
     4081 gccgccagaa gggaagggtg ttcatcctgg cacagcagtt gtgtgccagt gatgcgcagg
     4141 gtaacgcgag ccatcagagc tggaagttac tcaaatcgtc ggtgtccact tccgagtcaa
     4201 tctgcccgac cagataagaa ctgacttcca cttcctgcgg agcaacctgc acgttatcag
     4261 acaccagcca ggtattgatc cacgggatcg ggttggagcg cgtctggaac ggcagatcca
     4321 aaccgactgc ctgcatgcgg atattggtga tgtattcaac gtactggcag agaatgtctt
     4381 tattcagacc aatcatcgaa ccgtcgcgga acagataatc cgcccagtct ttctcttgct
     4441 gtgccgcctg gacaaacagg tcatagcact cctgcttaca ttcttcggca atttccgcca
     4501 tctccggatc atccgcaccg ctgcgcagca gattcagcat atgctgagta ccggtcaggt
     4561 gcagggcttc gtcgcgggca atcaggcgaa taattttggc gttaccttcc atcagttcgc
     4621 gttctgcaaa ggcaaaggaa caagcaaagc tgacgtagaa gcggatcgct tccagcgcgt
     4681 taacgctcat caggcagaga tacagttttt tcttcagctc gcgcaggcta acggttacgg
     4741 ttttaccgtt aacggtgtgg gtaccttcgc ccagcagatg ccagtagctg gtcatttcga
     4801 tcagctcatc gtaataactg gagatccctt ccgcacgttt ctggatctgc tcgttggtga
     4861 cgatatcatc aaacacaacg gacggatcgt taacgatatt acgaatgata tgggtataag
     4921 aacgagagtg aatggtttct gagaacgccc aggtttcgac ccaggtttcc agttcaggga
     4981 tcgagatcag cggcagcagt gccacgttcg ggctgcgacc ctgaatggag tccagcagcg
     5041 tctgatattt caggttgctg ataaagatgt gtttttcgtg ttcaggcagt gcctggtaat
     5101 cgatacggtc acgagaaacg tcaacttctt ccggacgcca gaagaaagag agctgctttt
     5161 cgatcagctt ttcgaagatg tcatattttt gctgatcgta gcgagccacg ttgaccggct
     5221 gaccaaagaa catcggttct ttgagctgat catttttcgt ctgtgaaaag gtggtatatg
     5281 ccatgaatgt gtcctgtggt tttattaccc ctcaccctaa ccctctcccg caaggggaga
     5341 ggggacggtt tggtgcaaag gctgggggat tatcagatct tgcatgcgcc gctttcgcag
     5401 ccatcgtcct ggattgacgg caccagatcg tcttgtgcgt cttcagcacc gtcacgggtg
     5461 ttctgataat acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcagt
     5521 tgctgcatcg gcacttttcc tgacgggaag cgtgacggat cgtagttggt gttagcagag
     5581 atcgactgat cgataaattt ctgcatgata cccaccagtt gcaggtagcc atcgttaccc
     5641 ggcatttccc acagcagctc ataggcgtcg tgcagatgct cgtagtccgg caccacctgg
     5701 cggagaatac cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg
     5761 ttagtggcgt tagagatctg cgaagaagtc tctgacggca tcagagcaga aagcgtggag
     5821 ttacgcaggc cgtgcgtttt gattgactca cgcagtgctt cccagtcgta atgcagcggc
     5881 tcattagcga tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg
     5941 taagtggttt cgttaaacca cgggcacgcg ccttgctctt tcgccagctc attagaggct
     6001 ttcagcaggt aatactgaat agcttcgaag gttttatgtg tcaggttgtt ggcgctgccg
     6061 tcggagtagc gtttaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc
     6121 agcgtacgac gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc
     6181 agcagcgcgt cgagtgcgcg tactgccaga atcgccagct cttccagttc atccaggcta
     6241 ttaattgcgc ccaggttgaa agcagacaac gtacacagcg cgatttcacc gttctcgtcg
     6301 ttgacatcat tcagcggttt ggtcggcagg gcgatttcca ggcacaggtt agactggcgc
     6361 actggcgcga tggccggatc aaacgggcta tgggtattgc agtggtcaac gttctgaata
     6421 tagattcgac cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc
     6481 acacgctgct tgcggatgct gtcgtctttc tcatatttgg tgtacaggcg ttcaaactct
     6541 tcctgatcgg cgaagaacgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg
     6601 atatcttcac ctttcagcag acgggtatac atcagtttgt tgatttgtac cccgtagtcc
     6661 atatgacgca cgcggttgcc ttccacccca cggttgtttt tcaacaccaa caggctttcc
     6721 acttccagat gccacatcgg gtagaacagc gtagccgcac cgccacgcac accgccctga
     6781 gagcaggatt tcaccgctgt ctggaaatgc ttatagaacg gaatacaacc ggtatggaac
     6841 gcttcgccac cgcgaatcgg gctacccagc gcacgaatac gcccggcatt gatgccgatc
     6901 ccggcacgct gggaaacata tttcacaatc gcgctggagg tggcgttgat ggaatccagg
     6961 ctgtcgccgc actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg
     7021 gacatgattg gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc
     7081 acgtattgca ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata
     7141 taaaggaact gggcgctttc gtagatttcg ccggtcacgc ggttctgtac cagatatttg
     7201 ccttccagct gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac
     7261 gtgtccatct gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg
     7321 cccatctcga ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag
     7381 gcttttttac gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca
     7441 tcacgggaga tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg
     7501 ataccgtcat aaaactgaat gtgggagcgc agttcgacct gggaaatcga aacgttatgc
     7561 agtccttctg ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg
     7621 ctaccgtcgc gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga
     7681 attctttccg caatagtttc atgcagacta cctgtagtaa gggaagctgc ttagaacact
     7741 atatataggg ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa
     7801 gtgcataact ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag
     7861 acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg
     7921 atttcttgaa cggaagaata ggttagataa attgatatag atggtttatt aatcttttgg
     7981 ctaatgattg caaaaattga tctaagcacg agtcatcaca taatctgatg tatcagttat
     8041 ggtcaatata taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctcttcgg
     8101 ttgttgttaa atgcgaagcc attttgttaa tttaccttat attataataa aattattcat
     8161 tactttaatt cagtttaaac ataactgata tcccttaagg tgattaactt gaattatgac
     8221 gcactgatat tattcatgaa ataataacaa aatagccatt gcaccgggtt gaacaattta
     8281 caaaagaaaa ataacccata tcgtataatg cgattaaata tgccgtctta tagaaaatga
     8341 tggtcagatt aatatgcgga ttatttttct acgcaaggag tatttatctt tactcccgtc
     8401 aatgattgca tctcttttct ctgctaacgg tgtcgcggcg gtcattgatt catgtcaggg
     8461 atatgatatc aaagcgagtt gtcacgccag caggcaaagc ctttcaggca ttacgcagga
     8521 ctggagcatc gccgatgggc aatggctggt tttttcggat atgaacaata acgccagcgg
     8581 tggggccgta tttttgcaac aaggagcgga gttttctcta tcgccagaaa atgaaactgg
     8641 aatgactctg tttgccaata atactgtctc aggagaatat aataacggcg gggcaatatt
     8701 tgctaaagaa aactcaacgc tgaatcttac tgatgttatt ttttccgata acgttgcagg
     8761 tggctatggt ggcgcaatct attcttctgg tactaacgat accggtgtcg tcgatttaag
     8821 tgtcactaac gccgtgtttc gcaataacat cgctaatgac ggcaaaggtg gtgcaattta
     8881 taccatcaac aatgacgtct atttaagtga agttattttt gataacaacc aggcatatac
     8941 atcaacaagt tacagtgatg gcgatggagg ggcaatcgat gtcaccgata ataatagcaa
     9001 tatcacgcat ccgtcaggtt tcacaataat aaataacacc gcatttacaa ataactctgc
     9061 tgagggatat ggtggggcga tttataccaa tagcgtgacg gctccctatc ttattgatat
     9121 ttcagttgat gacagctaca gccagaacaa cggcgtgtta gtggatgaga acaacagcgc
     9181 agcaggttat ggggatggtc cttccactgc ggcgggcggc tttatgtatc tcggcttaag
     9241 tgaagttacc tttgatattg ccgacggaaa aacgctggtt atcggcaata cagagaatga
     9301 cggagctgtt gactctattg ctggtaccgg ggtaatcacc aaaacaggtt ccggcgatct
     9361 ggtacttaat gcaaataaca atgactttac tggcgagatg cagatagaaa acggtgaagt
     9421 taccctgggc cgcagcaact ccctgatgaa tgtcggcgat acgcattgcc aggacgatcc
     9481 gcaagactgc tacggtctga cgatagggag tattgatcag tatcagaatc aggctgagct
     9541 aaacgttggc tcgacccaac aaacttttgt gcacgcattg acgggctttc agaatggcac
     9601 tttaaatatc gatgctggtg gcaatgttac tgttaatcaa ggcagttttg ctggcaccat
     9661 cgaaggtgct ggtcagctca ccattgcgca aaacggcagc tacgtgctgg caggggcaca
     9721 gtcgatggcg ctaactggcg atatagtcgt tgatgatggc gcggtgctta cgctggaagg
     9781 cgacgcggca gatcttgccg ctctccagga cgatccgcag tcgatcgtgt taaacggcgg
     9841 cgtgcttgat ctctctgatt tcgccacctg gcagagcggc acatcataca acgatggcct
     9901 tgaagtcagc ggcagcggcg gtacggttat cggcagtcag gatgtggtgg atctcgcagg
     9961 cggcgacaat ttgcatatcg gcggcgacgg gaaagatggc gtctacgtgg tgatcgatgc
    10021 gggcgacggg ctggttagcc tggcaaacaa taatagttac ctgggcacca cgcaaatcgc
    10081 ttccggtacg ctggtggtga gcgacaactc gcagcttggc aataccgacg aaaaccgtca
    10141 gctcatcttt actgatagcc aacaacaaag tgagatggaa attaccgctg acgttgatac
    10201 tcgttcagag gcagcaggtc acgggcgtga tattgaaatg cgcgccgacg gtgaagtggc
    10261 ggttgatgcg ggggtagaca cgcagtgggg cggactgatg gctgacagca gcgggcagca
    10321 tcaggatgag ggtagcacat tgactaaaac gggggcgggt acgctggaac tgaccgccag
    10381 cggtacgacg cagtcggcgg tacgtgtcga agaaggcacc ctgaaaggcg atgttgcgga
    10441 tatcttccct tatgcttcgt cgctgtgggt cggtgacggg gcaacgttca agactggcgc
    10501 ggatcaggat attcagtcaa ttgatgttac ttccagcggc actatcgaca tcagcgatgg
    10561 tacggttttg cgcctgaccg ggcaggatac ttccgtcgcc cttaatgcct cactgtttaa
    10621 cggcgatggg acgctggtga atgccaccga tggcgtgacg ttgacaggcg agcttaacac
    10681 caaccttgaa actgacagcc tgacttatct ttccgacgta acagttaatg gcaatctgac
    10741 caatacgtcc ggtgcggtca gcctgcaaaa tggcgtcgcc ggcgacacgc tgacggtaaa
    10801 cggtgattat accggcggtg gtacgctact gctcgatagc gaattaaacg gcgatgactc
    10861 ggcaagtgac caactggtgt tgaacggtaa tactgctggc aacacgaccg tggtaattaa
    10921 tcccattacg ggtattggtg agccgacatc tacaggcatt aaagtggttg atttcgcagc
    10981 cgatccaacg caatttcaaa acaatgcgca gttcagtctg gcgggcagcg gctacgtcaa
    11041 tatgggagcg tatgactaca cgctggtgga agataacaac gactggtatc tgcgatcgca
    11101 agaagcaacg ccaccatcgc cacctgatcc agacccgact cctgatccaa cacccgaccc
    11161 ggaacctacg cctgcctacc agccggtgtt gaatgccaaa gttggtggat attttaataa
    11221 cctgcgggcg gcaaatcagg cgtttgtgat ggagcgacac gatcacgctg gtggcgatgg
    11281 tcagacgctg aatttacgta ttatcggcgg agattatcat tacacagcag cggggcaact
    11341 ggctcagcat gaagatactt ctacggtgca gcttagcggc gacttgttta gcggacgctg
    11401 gggcgcggat ggcgagtgga tgcttggggc ggttggtggc tacagtgata accagggcga
    11461 cagccgctcg aatatgaccg gaactcgcgc cgataaccag aaccacggtt atgccgttgg
    11521 tctgacctca agctggtttc agcacggtaa tcagaagcag ggggcctggc tggatagctg
    11581 gctgcaatac gcgtggttta acaatgatgt ttccgaacat gaagatggcg tggatcatta
    11641 ccactcatcg gggattatcg cctcgctgga agccgggtat cagtggttac cggggcatgg
    11701 agtggtgatt gaaccacagg cgcaggtgat ttatcagggc gtgcagcagg atgattttac
    11761 cgccgctaac catgcgcgcg tgtcacaatc gcagggtgat gatattcaaa cgcggctggg
    11821 tttacacagc gaatggcgta ccgctgttca tgtcatacca tcattagatc tgaattatta
    11881 tcacgatccc catgcgacgg aaattgaaga agatggcagc actatcagtg acgatgcggt
    11941 gaagcaacgg ggtgaaataa aagtgggagt cacgggcaat atcagtcagc gagtttcgct
    12001 gcgcggcagc gtggcgtggc agaaagggag tgatgatttt gcccagacgg cagggttttt
    12061 gtcgatgacg gtgaaatggt agctgctttg cgcccggcat tgtagccgga tgtggtgcga
    12121 gcaccttatc cggctaacaa ttttatttat tctgcgtgtg cagcatatag ttcacatcga
    12181 cgccggggcc gagcttaaag gaattagtga tcgggttgta atgcagcccg gttatatgtc
    12241 gctcttttaa actggtctga tccacccagc ccagcagttc tgccggtttt ataaatttct
    12301 tcacgtcgtg cgtgcctttg ggcaccatgc gcaaaatata ttccgcgcca accaccgcca
    12361 tcagccatga cttgccgttg cggttaagcg tcgagaaaaa gacatcgccg cccggtttca
    12421 ccagttgcgc acaggctctg accactgact gcggatcggg gacgtgctcc agcatctcca
    12481 tgcaggtcac cacgtcatac tgcccggcat gttttgccgc gtgctcttcc acggtttcct
    12541 gcacgtactc tacctgaatg ccactttcca gcgcgtgcaa ctttgccacc tgcaatggct
    12601 caaagcccat atccagaccg gtcaccgtcg cgccttcgcg cgccatactc tcggccagaa
    12661 tgccgccgcc acaaccgaca tcgagcacct ttttgccaaa taaaccgcca gcacgctcgg
    12721 caatatagcc cagacgcagc gggttaatgc ggtgcagcgg tttgaactca ccttcaagat
    12781 cccaccagcg ggaggcgaca gcttcaaatt tagcgatctc ttcgtggtct acgttatggt
    12841 ttaccggcga tttttcggca ttcattggca cttctactcc gtaattggca agacaaacga
    12901 gtatatcagg cattggatgt gaataaagcg tataggttta cctcaaactg cgcggctgtg
    12961 ttataatttg cgacctttga atccgggata cagtagaggg atagcggtta gatgagcgac
    13021 cttgcgagag aaattacacc ggtcaacatt gaggaagagc tgaagagctc ctatctggat
    13081 tatgcgatgt cggtcattgt tggccgtgcg ctgccggatg tccgagatgg cctgaagccg
    13141 gtacaccgtc gcgtacttta cgccatgaac gtactaggca atgactggaa caaagcctat
    13201 aaaaaatctg cccgtgtcgt tggtgacgta atcggtaaat accatcccca tggtgacttg
    13261 gcggtttata acacgatcgt ccgtatggcg cagccattct cgctgcgtta catgctggta
    13321 gacggtcagg gtaacttcgg ttccatcgac ggcgactctg cggcggcaat gcgttatacg
    13381 gaaatccgtc tggcgaaaat tgcccatgaa ctgatggccg atctcgaaaa agagacggtc
    13441 gatttcgttg ataactatga cggcacggaa aaaattcccg acgtcatgcc aaccaaaatt
    13501 cctaacctgc tggtgaacgg ttcttccggt atcgccgtag gtatggcaac caacatcccg
    13561 ccgcacaacc tgacggaagt catcaacggt tgtctggcgt atatcgatga tgaagacatc
    13621 agcattgaag ggctgatgga acacatcccg gggccagatt tcccgacggc ggcaatcatc
    13681 aacggtcgtc gcggtattga agaagcttac cgtaccggtc gcggcaaggt atatatccgc
    13741 gcccgtgctg aagtggaagt tgacgccaaa accggacgtg aaaccattat cgtccacgaa
    13801 attccgtatc aggtaaacaa agcgcgcctg atcgagaaga ttgcggaact ggtaaaagaa
    13861 aaacgcgtgg aaggcatcag cgcgctgcgt gacgagtctg acaaagacgg tatgcgcatc
    13921 gtgattgaag tgaaacgcga tgcggtcggt gaagttgtgc tcaacaacct ctactcccag
    13981 acccagttgc aggtttcttt cggtatcaac atggtggcat tgcaccatgg tcagccgaag
    14041 atcatgaacc tgaaagacat catcgcggcg tttgttcgtc accgccgtga agtggtgacc
    14101 cgtcgtacta ttttcgaact gcgtaaagct cgcgatcgtg ctcatatcct tgaagcatta
    14161 gccgtggcgc tggcgaacat cgacccgatc atcgaactga tccgtcatgc gccgacgcct
    14221 gcagaagcga aaactgcgct ggttgctaat ccgtggcagc tgggcaacgt tgccgccatg
    14281 ctcgaacgtg ctggcgacga tgctgcgcgt ccggaatggc tggagccaga gttcggcgtg
    14341 cgcgatggtc tgtactatct gaccgaacag caagcccagg caattctgga tctgcgtctg
    14401 cagaaactga ccggtcttga gcacgaaaaa ctgctcgacg aatacaaaga gctgctggat
    14461 cagattgcgg aactgctgcg tattctgggt agcgccgatc gtctgatgga agtgatccgt
    14521 gaagagctgg agctggttcg tgaacagttc ggcgacaaac gtcgtactga aatcactgcc
    14581 aacagcgccg acatcaacct ggaagatcta atcacacagg aagatgtggt ggtaacactc
    14641 tctcaccagg gctacgtgaa gtatcagccg ctttctgaat acgaagcgca gcgtcgtggc
    14701 gggaaaggta aatctgccgc acgtattaaa gaagaagact ttatcgaccg actgctggtg
    14761 gcgaacaccc acgaccatat tctgtgcttc tccagccgtg gtcgcgtcta ttcgatgaaa
    14821 gtctatcagt tgccggaagc cactcgtggc gcgcgcggtc gtccgatcgt caacctgctg
    14881 ccgctggagc aggacgaacg tatcactgcc atcctgccgg tgaccgagtt tgaagagggc
    14941 gtgaaagtct tcatggcgac cgctaacggt accgtgaaga aaactgtcct caccgagttc
    15001 aaccgtctgc gtaccgccgg taaagtggcg atcaaacttg ttgaaggcga tgagctgatc
    15061 ggcgttgacc tgaccagcgg cgaagacgaa gtaatgctgt tctccgccga aggtaaagtg
    15121 gtgcgcttta aagagtcttc tgtccgtgcg atgggctgca acaccaccgg tgttcgcggt
    15181 attcgtttag gcgaaggcga taaagtcgtc tctctgatcg tgcctcgtgg cgatggcgca
    15241 atcctcaccg caacgcagaa cggttatggt aaacgtaccg cagtggcgga atacccgact
    15301 aagtcgcgtg cgacgaaagg ggttatctcc atcaaggtta ccgaacgtaa cggtttagtg
    15361 gttggcgcgg tgcaggtaga tgactgcgac cagatcatga tgatcaccga tgccggtacg
    15421 ctggtacgta ctcgcgtttc ggaaatcagc atcgtgggcc gtaacaccca gggcgtgatc
    15481 ctcatccgta ctgcggaaga tgaaaacgta gtgggtctgc aacgtgttgc tgaaccggtt
    15541 gacgaggaag atctggatac catcgacggc agtgccgcgg aaggggacga tgaaatcgct
    15601 ccggaagtgg acgttgacga cgagccagaa gaagaataat tttacttctt catgccaaaa
    15661 gggagctatc tcccttgttt gaattgaaaa gtccaggctg caaagtctgg gcttttgtcg
    15721 tattagggcg cggtaaagtt ttgctgtgct cataaaaaat ggctggcttt acacaaggaa
    15781 tgtggcaatg agtggtgaaa aaaaggcgaa aggctggcgg ttctatggtc ttgtaggttt
    15841 gggcgcaata gtactgcttt ccgctggcgt ctgggcgttg caatatgctg gcagtgggcc
    15901 agaaaaaacg ttgtcgccgc tggtggtgca caacaatctg caaatcgatc tcaatgagcc
    15961 ggacctcttt ctcgacagcg actctctgag ccagcttccc aaagacctcc ttaccattcc
    16021 gtttctccac gatgttctga gtgaagattt cgttttctat tatcagaatc atgccgatcg
    16081 tctgggcatt gaaggcagca ttcgccgcat tgtctatgaa cacgatctca cgctgaaaga
    16141 taagctcttt tcgtcactct tagatcagcc cgcgcaggcg gcgctatggc acgataaaca
    16201 aggccatctt tcacattaca tggtgctgat ccagcgcagt ggtttaagca aactgctgga
    16261 gccattgttg tttgccgcta ccagcgacag ccagttaagc aaaacggaaa tcagtagtat
    16321 taagctaaat agtgaaacca tcccggttta tcagttgcgc tataacggca ataacgccct
    16381 gatgttcgcg acttatcagg acaagatgct ggtgttttcc agtacggata tgctgtttaa
    16441 agatgaccag caggataccg aagccacggc gatcgcaagt gatttgttga gcggtaaaaa
    16501 acgctggcaa gccagctttg gcctggaaga gcgtactgcc gaaaaaacgc cagtacgcca
    16561 gcgcatcgtg gtcagcgcca ggttgctggg gtttggctac cagcgattaa tgccttcttt
    16621 tgctggcgta cgcttcgaaa tgagcaacga cggctggcat agctttgtgg cgttaaatga
    16681 tgaatccgca agcgtagatg ccagtttcga ttttacgccg gtatggaaca gtatgcctgc
    16741 cggggccagc ttctgtgtgg cggtgccgta ttcgcatggt attgccgaag agatgctttc
    16801 gcacatcagc caggaaaacg acaagctgaa cggggcgtta gatggtgcag ccgggctgtg
    16861 ctggtatgaa gactcaaaat tacaaacccc gctgtttgtc ggtcagtttg atggcactgc
    16921 cgaacaggca caactgcctg ggaaactgtt tacgcaaaat attggtgcgc acgaaagcaa
    16981 agcgccagaa ggtgttttgc cggtaaacca gactcagcag ggcgaagcgc aaatctggcg
    17041 tcgcgaagtg agttcccgat acggccagta tccgaaaaca caggcggcgc aaccagatca
    17101 actaatgtcg gattattttt tccgcgtgtc gctggcgatg caaaacaaaa cgctgctttt
    17161 ctccctcgat gacacgctgg ttaataacgc gctgcaaaca ctgaataaaa cccgcccggc
    17221 aatggtggat gtaataccca ctgacggcat cgttccgctc tatatcaatc cacaaggcgt
    17281 ggcgaaactg ctgcgtaacg aaacgctgac cagtctgccg aagaatctcg aaccggtttt
    17341 ttataacgcc gcacaaactt tattaatgcc gaagctggac gctttatctc aacaaccgcg
    17401 ttatgtcatg aagctggcac agatggaacc cgatgccgcc tggcagtggc tacccataac
    17461 ctggcagccg ctatgaggca cggactgctg gcgctgattt gctggctgtg ttgtgttgtt
    17521 gcccatagcg aaatgctgaa tgtcgaacag tcggggctgt ttcgcgcctg gtttgtgcgt
    17581 attgcacagg aacagctccg acaggggcca agtccgcgct ggtatcagca ggattgtgcg
    17641 ggcctggtgc gattttcggc gaacgaggcg ctgaaagttc acgacagcaa atggctaaaa
    17701 agtaacggca tagcaagtca gtatttgccg cctgaaatga cgctaacacc tgaacagcgt
    17761 caactggcgc aaaactggaa tcaggggaac ggaaaaaccg gcccctatgt gaccgcgatt
    17821 aatttgattc agtacaacag ccagttcatt ggccaggaca taaaccaggc gctgcctggc
    17881 gatatgattt ttttcgatca gggcgatgcc cagcacttaa tggtctggat ggggcgttac
    17941 gtcatctacc acaccggaag cgccacgaaa actgacaacg gaatgcgcgc agtcagtctg
    18001 caacaactta tgacatggaa ggacacccga tggataccca acgattccaa tcccaatttc
    18061 attggcattt atcgtttaaa ttttctggcg cgatagcagc gtgcttgtcc ctgtctcttg
    18121 tgggaacagg gcttgctaat gctgatgatt cgcttccttc cagtaactat gcgccgcccg
    18181 cagggggaac attctttttg cttgctgaca gcagttttag cagcagtgaa gaggcgaaag
    18241 tgcgactgga agcgccgggg cgtgattatc ggcgctatca gatggaagag tacggcggcg
    18301 tggacgttcg cctgtatcgt attcctgacc cgatggcatt tttgcgccag cagaaaaacc
    18361 tgcatcgcat tgtggtgcaa ccgcaatatc tgggcgacgg gctgaacaat acgctgacct
    18421 ggctgtggga taactggtac ggcaaatctc gccgcgtgat gcagcgtact ttctcttctc
    18481 agtcacggca gaatgtgact caggcgttac ccgaattaca gctcggcaat gccattatta
    18541 aaccttcccg ttatgtacag aacagccagt tttcaccgct gaaaaaatat ccactggtgg
    18601 aacagttccg ttacccacta tggcaggcta aaccggtcga gccgcagcaa ggggtaaaac
    18661 tggaaggcgc atccagcaat ttcatctcgc cgcagccggg taacatctat attcctctcg
    18721 gcaaacaaga gccgggactg tacctcgtcg aggcgatggt cggtggatat cgggcgacga
    18781 cggtggtatt tgtctctgat accgtggcgc ttagcaaagt gtcgggtaac gagctcctgg
    18841 tgtggaccgc gggtaaaaaa cagggtgaag cgaagccggg atcagagatc ttgtggactg
    18901 acggtcttgg cgtgatgacc cgcggtgtga ccgatgacag tggtaccttg cagttacaac
    18961 atatatcgcc ggaacgttca tacattctgg gtaaagatgc tgaaggcggc gtttttgtct
    19021 ccgagaattt tttctacgaa agcgaaattt acaacacccg cttgtatatc ttcaccgacc
    19081 gccctctgta tcgcgcaggc gatcgtgttg atgtcaaagt gatgggccgc gagttccacg
    19141 acccgttgca ttcatccccc atcgttagcg ccccggcgaa gctttcagtg ctggacgcca
    19201 acggcagtct gttgcaaacc atcaatgtca cgctggatgc gcgtaatggc gggcagggga
    19261 gtttccgcct gccagaaaat gccgtcgccg gaggttatga gttacgcctg gcttaccgca
    19321 aacaggtcta tagcagcagt tttcgcgtag ccaactacat caaaccacat tttgagattg
    19381 gcttagccct cgacaaaaaa gatttcaaaa ctggcgaagc ggtcagcggc aaactgcaac
    19441 tactctatcc ggatggcgaa ccggtaaaag atgccagcgt gcagttgagt ttgcgcgctc
    19501 agcaactttc gatggttggt aacgatttgc gttatgccgg acgtttcccg gtgtcgctgg
    19561 aaggtagcga cgctgtttct gacgataaag gcaatgtagc gttaaacctt cccgccgccg
    19621 ataaaccgag ccgctattta ctgaccgtct ccgccagcga tggcgcggca tatcgtgtta
    19681 ctactaccaa agagatcctc attgaacgcg gtctggcgca ttactctcta agcacctccg
    19741 cacaatacag taatagcggc gagtcggttg ttttccgtta tgccgcgctg gaatcttcaa
    19801 agcaggtgcc tgttaagtat gagtggctac gcctggaaga ccgaacaagc cacagcggag
    19861 atctaccttc tggtggcaaa tcctttaccg tcaatttcga taaaccgggt aactacaatc
    19921 tgacgttacg cgataaagac ggcttaattc tcgccgggtt aagccatgca gtcagcggta
    19981 agggcagcac gtcgcatact ggtacggtag atattgtggc agataaaacg ctgtaccagc
    20041 ctggcgaaac cgcgaagatg ctgattacct tcccggagcc aattgatgaa gcattattga
    20101 cgctggaacg cgatcgcgtt gaacagcagt cgctgctatc gcatccggca aactggctga
    20161 cgttacaacg tttaaacgat acccagtatg aagcccgcgt accggtgagc aattcctttg
    20221 cgcctaatat cactttttcg gtgctgtata cccgtaacgg tcagtacagt tttcagaacg
    20281 ccgggatcaa agttgccgtt ccccagctgg atatccgggt gaaaacggac aaaacccatt
    20341 accagcctgg tgaactggtc aatgtcgaat taacctcgtt gctgaaaggt aaacctgttt
    20401 ctgcgcagct aacggttggc gtggtcgatg aaatgatcta tgcgctgcaa ccggaaatcg
    20461 cgccgaatat cggcaaattt ttctatccgc tggggcgtaa caatgtgcgt accagctcca
    20521 gtctttcgtt tatcagctac gaccaggcgc tctccagcga gccggttgcg ccaggcgcga
    20581 ctaaccgcag cgagcggcga gtaaaaatgc ttgaacgtcc acggcgtgaa gaggtggata
    20641 ccgcggcatg gatgccgtca ctcacaactg ataaacaagg caaagcgtat ttcacgttcc
    20701 tgatgcctga ttcgttaacc cgttggcgca ttacagccag agggatgaac ggcgacgggc
    20761 tggtcgggca ggggcgtgct tatcttcgtt cggaaaaaaa tctctacatg aagtggagta
    20821 tgccaacggt gtatcgcgtg ggcgacaaac cggcggcagg actgtttatc ttcagtcagc
    20881 aggataacga accggtggcg ctggtgacta aatttgcagg cgctgagatg cgccagacgc
    20941 tgacgctgca caaaggggcg aattatattt cgctgacgca gaatattcag caatctggct
    21001 tgttaagtgc agaactgcaa caaaatgggc aagtgcagga cagcattagc acaaaactgt
    21061 cttttgtgga taacagctgg cccgttgaac agcagaaaaa tgtcatgctc ggcggtggcg
    21121 agaacgcgct gatattgccc gagcaggcga gcaatatccg gctacaaagt agtgaaacgc
    21181 cgcaggagat tttccgcaac aatcttgatg cgttagtcga tgaaccgtgg ggtggggtga
    21241 tcaacaccgg tagccgtctg atcccgctca gtctcgcctg gcgttcgctt gccgatcatc
    21301 aaagtgccgc cgctaacgac attcgtcaga tgattcagga taaccgtctg cggctgatgc
    21361 aactggcggg gcccggagca cgctttacct ggtggggtga agatggcaat ggcgacgcct
    21421 tcctgactgc atgggcatgg tacgccgact ggcaggccag ccaggcgctc ggcgtaacgc
    21481 aacaaccgga atactggcag catatgctcg acagttatgc cgagcaggca gataacatgc
    21541 cgttattgca tcgggcgctg gtgctggcgt gggcacagga gatgaatctg ccgtgcaaaa
    21601 cgttgttgaa agggttggat gaagctatcg cccggcgcgg aactaaaact gaagatttct
    21661 ctgaggaaga cacccgcgat atcaatgaca gcctgatcct cgatacaccg gaatctccac
    21721 tggcagatgc ggtggcaaac gtcttaacca tgacgttgct gaaaaaagcg cagttgaagt
    21781 ccactgtgat gccacaggtt cagcaatatg cgtgggataa agcggcaaac agcaatcagc
    21841 cgctggcgca cacggttgtg ctgctcaata gcgggggcga cgctacccag gcggctgcta
    21901 ttttaagtgg tttgaccgct gagcaatcta ctattgagcg cgcgctggcc atgaactggc
    21961 tggcgaaata tatggcgaca atgccttcgg ttgtgttgcc tgcgcctgcg ggcgcatggg
    22021 ccaaacataa gttaactgga gggggcgaag actggcgttg ggttggtcag ggcgtgccgg
    22081 acattctctc ttttggtgac gaattatcgc cgcaaaatgt gcaggtccgc tggcgtgaac
    22141 cggcaaaaac ggctcaacaa agtaacattc cgatgaccgt tgaacgccag ttgtatcggc
    22201 ttatccccgg tgaagaagag atgagcttta ctctgcagcc ggtgaccagc aatgagattg
    22261 acagcgatgc gctgtatctc gatgaaatca cgcttaccag cgagcaggat gcagttctgc
    22321 gctatggtca ggtggaagta ccgctcccgc cgggagccga tgttgagcgc acaacatggg
    22381 gcatttcggt caataaaccc aacgccgcga aacagcaggg gcaattgctg gaaaaagcgc
    22441 gaaatgaaat gggcgaattg gcctatatgg tgccggtgaa agaactgacg ggaacggtca
    22501 ctttccgcca tttgctgcgc ttctcgcaaa aagggcaatt cgttctgcct cctgctcgtt
    22561 atgtgcgttc ctatgcacct gcacagcaaa gtgttgcggc agggagtgaa tggaccggga
    22621 tgcaggtgaa ataagtgaac tggcgcagaa ttgtctggct attggcgttg gttactctgc
    22681 caacgcttgc ggaggagacg ccgttacaac tggtgctgcg cggtgcgcag cacgatcaac
    22741 tgtatcagct gtcgtcatca ggcgtgacca aagtctcagc attacctgac acgctcacca
    22801 cgccgttagg gagtttgtgg aaactgtatg tttacgcctg gctggaggat acccatcaac
    22861 cggagcagcc ctaccagtgt cgcggaaact ccccggaaga ggtttattgt tgtcaggctg
    22921 gggagagcat aacgcgcgat accgcgctgg tacgttcctg tggactctat tttgcgccgc
    22981 agcggttgca tattggcgca gatgtgtggg ggcaatactg gcaacagcgt caggcaccgg
    23041 cgtggttagc gtctcttact acgctgaagc cagaaacctc ggtaacggtt aaatcactgc
    23101 ttgattcatt agcgacgtta cccgcgcaaa acaaagctca ggaagtctta ctggatgtgg
    23161 tgctggacga ggcgaaaatc ggtgtcgcat ccatgctcgg tagtcgggta cgggtgaaaa
    23221 cctggagctg gtttgccgat gataaacagg aaatccgtca gggtggtttt gccggctggt
    23281 taaccgatgg ttcaccgctg tgggttactg gcagtggcac cagcaaaacg gtactaaccc
    23341 gttatgcgac ggtgcttaat cgggttttgc cagtgccgac gcaggttgcc agtgggcaat
    23401 gtgtagaggt ggagctgttt gcccgctatc cgctgaagaa aatcacagcg gaaaagagta
    23461 cgacggcagt aaaacccggc gtacttaatg ggcgctatcg tgtgacattt gccaatggta
    23521 accacattac gtttgtcagc cacggtgaga cgaccttact gagcgaaaag ggcaaactga
    23581 aattgcagtc gcatcttgat cgtgaagagt acgttgccag agttctggat cgcgaggcga
    23641 aaagtacgcc ccctgaggca gcgaaagcca tgaccgtggc gattcgcact tttttacagc
    23701 aaaacgccaa tcgggaaggc gattgcctga ccatccccga cagtagcgcc acgcagcgtg
    23761 tttctgcttc gccagcgacc acgggggcgc gaacgatgac cgcctggacg caggatctta
    23821 tctatgcggg cgatccggtt cattatcatg gcagtcgcgc taccgaaggg acgctttcct
    23881 ggcggcaagc cacggcccag gcggggcagg gagagcgtta cgaccagata ctcgcgtttg
    23941 cttatcccga caacagcctt agccgctggg gagcgccacg ctcaagctgc cagttattac
    24001 ccaaagcgaa agcctggctg gcgaaaaaaa tgccgcagtg gcggcgtata ttacaaggcg
    24061 agacggggta caacgaacca gacgtgtttg cggtctgtcg tttagtctca ggtttcccct
    24121 ataccgatcg gcagcagaaa cggctgttta ttcgtaactt ctttacgctt caggatcggc
    24181 tcgatttaac ccatgagtat ctgcacctgg cctttgatgg ttatcccacc gggcttgatg
    24241 agaactatat cgaaacgctg acccgacaat tattaatgga ctaatgctat gcgaaagatt
    24301 tttcttccgt tgttactggt ggcgctttcg cctgtcgctc acagtgaagg tgtgcaggaa
    24361 gtcgagattg atgcaccgct ctctggctgg catccggcag agggcgaaga tgccagtttt
    24421 tcacaatcga tcaattaccc ggcgtcgtcc gtcaacatgg cagatgatca aaatatctca
    24481 gcgcagatcc gcggcaaaat aaagaattat gctgcggcgg gtaaagttca gcagggccgg
    24541 ctggtggtca acggtgccag tatgccgcag agaattgaat ccgatggttc atttgcacgc
    24601 ccttatattt ttactgaagg cagcaacagc gtgcaggtca tcagcccgga tgggcaaagc
    24661 cgacaaaaaa tgcagtttta ctcaacgccg ggcgctggag cgatccgcgc gcgtttacgg
    24721 ctggttctct cctgggacac ggacaatacc gacctcgacc ttcacgttgt tacacctgat
    24781 ggcgaacacg cctggtacgg taacagcgtg ctgaaaaaca gtggtgcact ggatatggat
    24841 gtcacgacgg ggtacggccc cgagattttc gccatgccag cgccgattca cggacgttat
    24901 caggtgtata tcaactacta tggcggacgc agcgaaacgg aattgacgac cgcccaactg
    24961 acgctgatca ccgaggaagg atcggtcaat gagaaacagg aaacatttat tgtaccgatg
    25021 cgtaatgctg gcgagctgac gctggtgaaa agttttgact ggtgattaat ggggttacct
    25081 tcgccgtcga tgttcaagaa aacgcccgat aactttcgct atcgggtgtt tttattgatt
    25141 aattcaaccg ttcaatcacc atcgcaattc cctggccgcc accaatgcac agtgttgcca
    25201 gccccagcgt tttatcgcgt gcctgcatgg catgtaatag tgtgaccaga atacgagcac
    25261 cactggcacc gataggatgc ccgagcgcga tggccccgcc gttgacattc actttctcag
    25321 aatcaaagcc cagggttttc ccaacggcaa ggaactgtgc agcaaatgct tcattagcct
    25381 caatgagatc aatatccgcc agttgcagcc ccgccagttg taacgctttt tgcgtggcag
    25441 gtactggccc catacccata aatgcggggg gcacgccacc gctggcataa cttttaatgc
    25501 gagccagggg ggtaaggcct gctgccagcg ccgcagattc ttccataatc accagagcgg
    25561 cagcaccgtc gttaatacca gacgcgttcc cggcggtgac tgttcctgct ttatcgaagg
    25621 ccgggcgcaa tgcgcctaac gcttcagtcg tagaatccgc tttcgggaat tcgtcttgac
    25681 tgaagacgaa ggttttcttc cgagtgacaa catttaccgg gacgatttcg gctgtaaaag
    25741 caccggactc aattgcggct gccgctttac gctgtgaatg tagcgccagt tcatcctgca
    25801 tttctcgggt aattccgtac tctttggcca cgttctcggc ggtaatcccc atatgataac
    25861 catgggtggc gcacatcaga ccatcgcgca ggattacgtc ataaacctgt ccgtctccaa
    25921 gacgataacc agagcgtgct tttgcatcga gtaagtaggg cgctaaactc atattttcca
    25981 taccccccgc cacaatgctt tgcgcctgac ctgcctgaat tgcctgggcg gcaagcgcca
    26041 cacttttaag acccgaacca cacactttat tgaccgtgaa tccgcacacc gtttctgcca
    26101 gcccgctttt taacagtgcc tgacgtgccg gattttgccc caacccggct tgcaacacgt
    26161 tacccataat cacttcatca atgtgtagtg aatcgattct tgcacgttca atggcggctt
    26221 taattactgt cgcccccagg tcgatggcgc tggtggaagc gagtgagccg ttaaaactac
    26281 cgatagcagt acgtaccgca ctgacgatga cacaattttt cattttttat tccttctgtc
    26341 tgtagggttg ccgtcagaac agcgttaaac caatgacgaa aatgacgccg gagaagagca
    26401 gggcagtgat gcaatagccc atgatgtcgc ggacaccgag tccggcgatt gccagtgctg
    26461 gcagcgccca gaatggttgt gccatgttca tccattgctc gccgtaggcg atcgccatta
    26521 ccgatttacc gagatcagcg cccagcgcct gggctgcggg tatcacgaaa ggtccctgaa
    26581 taacccagtg accgccgcca gacggaacgg cgaagttaat cagtgcagaa ctaaaaaagg
    26641 tcattaccgg gaaggtgtct ttgttcgcaa cattgatgaa gaattcggta atgagtccgc
    26701 ccagaccgga gtgctccatc atcagttgga tcccagcgta gaaggggaat tgcaccaaaa
    26761 taccggcagt actgcgtgct gccgcgctga tagcacgcat ataagccatt ggcgttttat
    26821 gtagcagcag acccgcaatc ataaacatca ggttgacggt attgatggtg atgttgaagc
    26881 catgttcgct gaagtacatc gcaaggtaag cgataccgag tgcgccgatg atcaacgtca
    26941 gaatgcggct ttcttccagt cgctcggatg gtggggcatc tttcggtagc tgcttttgga
    27001 aatcagcctc ttccatgagt agtttcggat cgatactcac cacgtcagac ggttttggca
    27061 tcatcatgcg ggtgataaat ggcatcacca caatcaacgc cacagtgatg aaaatattaa
    27121 aaccactgaa cagagtatcg cccaccggga tcagcccggc gatatgctca accgggttgc
    27181 ccggtgttgc agccaacaga ggcattgagc cagagaaacc gccaccccag gtgagaaaac
    27241 caatgtaggc gcaggcaatg agcaacggat aatcagaacc gggtactcgg cgggcgactt
    27301 cacgggcaaa cattgcgccg acaaccaaac caaatcccca gttgatgaca caagcgactg
    27361 aaccgaagaa agtaaccagc atgacgccct gtacgggcgt ctttgcggcg gaggcggcag
    27421 tacgcagcaa actttttacc ggagcagagc tggcaagggc atgaccggta acgatgataa
    27481 gcgccatctg cataccaaac gccagcaagt tccagaaacc gtcaccccac attttcacca
    27541 tgctgatcgg cgtttgtggt gttaaccaaa gcgcgatcac gaaagttagc aatgtcagca
    27601 acatggcaaa gatcagtgga tcgggaagcc accggctgac aaaacgcgtc ataaaacgcg
    27661 atatgcgacc aatcataaat caccccgttg cgtattcaga tcggcggcga cttcaaaccg
    27721 agcttctgtt ttggcacgca cggtggctaa atcacacccg tcggcaattt cggtgagcca
    27781 cattttgccg tcaataaaac gaaagacagc cagttcagta accagcatat gcaccgcatg
    27841 ttgcgcagtg agtggcatgg tgcagcggcg caaaattttt gctgaaccat ctttggcaca
    27901 atgttccatg gcgatgatca ctttgcgcga cccggtcacc agatccatcg cgccacccat
    27961 accgggcacc attttcccag gcactaccca gttcgcgagg tttgcttctt cgtctacttg
    28021 caaaccgccg agcacgcagg catcaatatg accgccgcgg attagcgcaa atgacatggc
    28081 gctatcaaac atggctgcac cgggtaaaac accgcacggt tgtccgccag cgttcaccag
    28141 atctggatgc gctgtcgtga ccgggcctaa accgaggaag ccgttttccg attgcagagt
    28201 gatatgaata ccctccggta aataattggc gaccattgtg ggtaaaccga tccctaagtt
    28261 aacgatgtca ccatcacgaa gctcttgcgc cacacggcgc gcaatacgtt gtttcgcatc
    28321 cattatttgc tctcctgtga aacgatgatg tggtcgataa cggcaccagg ggtgacaata
    28381 tggtcaggtt gcagctcgcc ggtttcgacc agttcatctg gctctaccag tgtgatatca
    28441 gccgcaaggg ctatcagggg gttaaagttg cgggcgctaa gttgataggt caggttgcca
    28501 agtgtgtcgc aacgatgagc gcgaattagc gccaggtcgg cgcgcagtgg gcgttcgagc
    28561 agccaggttt taccgtcgag tgtcagtgtc tgtttgcctt cctctacgac ggtgccgaca
    28621 cccgttgggg tgagaaaacc accaagtcca gctccaccac agcgaatttg ctcgattagc
    28681 gtaccttgcg gcaccagaac gacgtccatc tcaccagata tcatgcgccg acctgtttcc
    28741 gggttggtgc cgatatgtga agcaatcact ttgcggactc gaccattgac gatgagcgga
    28801 ccgatgccag tatcaacaaa cgcggtatca ttggctatca atgtcaggtc gcgaacacca
    28861 gattccagta atgcttcaac caggcgggat ggagtgccaa tccccataaa tccgcccacc
    28921 atgatggtca tgccgtcacg aaagaagccg gtggcatctt gtaatgtcat caattttgtt
    28981 ttcatttttt atccctcttg catacaccgt tgtgggtaca gtcagggaat agcaagggga
    29041 gtgccagaat gagattattg ggttttgtta tttaactaat tgaatatgaa gggataaatt
    29101 ggatgaggat ttataaggag aatgagcggc tatgcagaaa attgcgcact gtgcaaatat
    29161 ctgcatagca agttttggcg ttatacatcc gccggatcga taccgtattc ctggagttta
    29221 tacatcaatg cacggcgact gatgcccagc attaaagcag tgcgggttcg gtttccttct
    29281 tgttgttcca gcacttccat aatgatgcgt ttttcgacgc gtttaatttc ctcttttaaa
    29341 ttacgctcac cgacaggggc tgtttttgcc tcaccagcat tacagactgg ctgacgaatc
    29401 tgcggcggaa gatcctcaga gaaaatgatc gggcctgagt tcatcacgac ggcgcgttca
    29461 ataacgttgg aaagctctcg aatatttccc ggccatgacc aggcggtaag cagtgacatt
    29521 gccatcggat cgatgtcgat aatatcgcgc tgattctcac tactgaattt ttgcaaaaag
    29581 tgattagcta acagggaaat atcttcccgg cgatcgcgca gaggcggcag tattaaatga
    29641 ataacgttaa ggcgataaaa gagatcttca cggaaggtgc cttcttttac cattgcctgc
    29701 aagtcacggt tggtggcagc aatgatgcgg atatcaactt ttatggtctg atggccgcca
    29761 atccgttcaa attcccgttc ctgcagaatg cgtagtaatt tggcttgcag tacgagcggc
    29821 atttcgccaa tttcgtcgag gagcagagta ccttcgttgg cacgttcaaa taatccctga
    29881 cgcaaggttt gtgcaccagt aaatgcacct ttttcatgac caaacagttc actttcgagc
    29941 aacgattccg gcagcgccgc gcagttgact ttaatgaacg gcccctttgc ccgccgcgaa
    30001 ttgtagtgaa tcgctctggc aatcaactct ttcccggtgc cgctttcacc gctaatcaag
    30061 acgctggcct gagaaagggc aattttggcg gtgtctttgc agatatccat catcgcaggg
    30121 ctgttggtga gaatgtgccc ccattgccag ctggtgctca gtgcctggtg cagatgacgg
    30181 atctcttttt tcattgactg gagttgtaaa gcgcgctgaa cgattaaatt caactcatcg
    30241 agatcaaacg gtttgataac atagtcgaag gccccgcagc gtagcgcttc gacggcggtt
    30301 tccacttcag cataggccgt catcagaata acgggggtcc gtgtctcatg gctgcgcatc
    30361 tccttgagtg ccttgatgcc gtccatctct ggcatgcgga tatccatcaa caccacgtca
    30421 gggtgaatat cggcaaacag gtgtaatgct gtgcgtccgt tgttcgcaca atgtgtttcg
    30481 aatccttgta gtgcaaaagc ggtgctcagc atacggcgaa cattatcttc atcatccaca
    30541 ataaggatgc gattaatagc agtcatacag tctgatttcc ctgcggattg atcggtaaaa
    30601 taagcgtgaa ggttgcgccg tagcccggca aactggcgac gcgaatatca ccctgatggg
    30661 cattaatgat gcgttgactt aacgccagac caagcccggt tccagaggct ttggtggtga
    30721 aaaagggatc gaagatcttt ttttgcagcg agagatcaat gccacagccg ttgtcctcta
    30781 tcgaaatggc ctgttgtgag tcgctgtatt gccaggtttg aatgcgaatt ttccctcgtg
    30841 cgctgatagc ctggacggca ttgatcagga tattcagtag tacctgtttg agcagttcac
    30901 gatcggcgtt aatcgggttt aattcattat ccagttcgct tatgaagtcg acccgcgctt
    30961 gtacgccggc ggtttgtacc agtaccagag tttcttcaac caatgcattg agactgactt
    31021 gttgccattg actgtggcgt ggacgtgaaa attcgagcaa ttgctgaata actttgttaa
    31081 ttgaatcgat ttctttgagt actacggaca gatattcctg atgtattagg tcgctggttt
    31141 gttggcgcaa gatctgtaca taaccacgaa tagccgttag cggattacgt acttcatgcg
    31201 cgacgccagc catcagctca cccagtgtgg cgaggcgttc tgcttgcgcc atgcggcgct
    31261 gggtttcttt gcgggcagtt aaatcagaga aaatcaccaa agcacctatc atttcaccat
    31321 gcgtgttatg aatacgactg gtagtgacac tgagttcaat ggtgcggtca cggccaggaa
    31381 aactgatctc cagcgctaca tgttcggtgc catgttccag cgtatccagt actggactgt
    31441 agaactgagt attgtcgaac aacatggagt aaggctgccc taccagttca tgacgttgat
    31501 agccagtgat gacttctgct gccgggttca tggtggttac atcaccctgg cggtcaatgg
    31561 caatgacgcc atcggcagcg ttttcaataa tcagatcgtt aagtgtccgc gtttcacgca
    31621 gtgcctgggc gaggttgtta acactctgac tgatttgccc catttcaccg ggcaattgtg
    31681 gtaatcgagt gggaatattt tgtgccagag tcgagaggcc atcggtgatg atatcaatat
    31741 tggcgctcag gcgacgggag aaaaggacaa tcaacagcag gcttatcagc aaaccggcgg
    31801 tgagcacaat gataatcctc acatccattt tccaggcctg gcggcgaata tcttcggtta
    31861 attcattggc ccagatatag ccgaggattt caccattacg ctcaatgggg atcattgaat
    31921 tcaaaatatc gccgcgcacc tgcctgcctg aataaaccaa aggggtattt gtacgcatga
    31981 cttcgcgacc agggtgatct gcggcaatgg taacgccgac attattctga tatagcgctg
    32041 aaggcgcgta ggtgattatc gcatccagcg ttttgttgta ataaccagcg ccgatgccag
    32101 ggaaggcgtg agtgatattt tcggtaatgg gggcaagttc tgcatttaat gcgcggatac
    32161 gctcctcacg tggtaagtcg atgtagagat catagcgatc gcctagtgcc tgattaagca
    32221 ggttgaccac ggcagaaagt tttttctctt tttcagataa gactgctgaa cgtccttccg
    32281 tttctacgat ataaccaata gtaagcgttg ggacaatgac catcaggatt gccatcagaa
    32341 tcatttgatt gcgtaagcgg cgtggataaa tccacttcat ataatgcatg ctgaaatcca
    32401 ctagtcttgt ccggtatatg acgattatca gaggttaagg tgatgatttc tcggcggtgt
    32461 atcatattcc agagaagaga gaacattgcg gtaacacgct tttaccgcta ccttaaccac
    32521 actccatcgg tcacctgagg cggagcttcg cccctttgaa ataccttgct tcttttcgta
    32581 caaccctgaa agcctcgcgc tacatgttca gagcattggc gttagtgctc tggctgttga
    32641 ttgctttttc atccgttttt tacatcgtta atgcgttaca tcagcgagaa tcggaaattc
    32701 gtcaggaatt taatctgagt tccgatcagg ctcagcgctt tattcaacgt acctctgatg
    32761 tgatgaaaga gctgaagtac atcgctgaaa atcgcttatc ggcagaaaac ggtgtgcttt
    32821 ccccgcgtgg acgagaaacg caggcggatg tgcctgcgtt tgaaccgctg tttgccgact
    32881 ccgattgttc cgcaatgagt aacacctggc gaggctctct ggagtcattg gcgtggttta
    32941 tgcgctactg gcgcgataat ttctctgcgg cttacgatct caaccgggta tttttaatcg
    33001 gcagcgataa cctctgcatg gccaatttcg gtctgcgtga tatgccagtg gaacgcgata
    33061 ccgcgttgaa agcgttgcat gaacgcatta ataaatatcg aaatgcacca caagatgata
    33121 gtggcagtaa cctctactgg atcagcgaag gtccgcgccc tggcgtcggg tatttttacg
    33181 cgttgacgcc agtttatctg gcgaaccggt tgcaggcgct tttgggtgtc gagcagacta
    33241 tccggatgga gaattttttc ttacctggta cgttgccgat gggggttacc attcttgatg
    33301 aaaatggtca taccctgatt tcgcttaccg gaccagaaag taaaattaag ggcgatcctc
    33361 gctggatgca ggaacgctcc tggtttggct atacggaagg gttccgggag ctggtgctga
    33421 agaaaaatct gccaccctca tcgctaagca tcgtgtattc ggtgccggtt gataaggtgc
    33481 tggaacgcat tcgcatgttg atccttaacg caattttgct gaatgtgctt gccggagctg
    33541 cattgtttac tctcgcgcgg atgtacgagc gacgtatttt cattccggcg gaaagcgacg
    33601 ccctgcgact ggaagaacat gagcagttta atcgcaagat tgtcgcctcc gcgccagtgg
    33661 gtatctgcat tttgcgtacc gctgatggcg tcaatatttt aagtaacgaa ctggcgcata
    33721 cctatctcaa tatgcttacg catgaggatc gccaacggct gacacaaatt atctgtgggc
    33781 agcaggtcaa ttttgttgat gtcctgacca gcaacaatac caatctgcaa atcagcttcg
    33841 tccattcgcg ctatcgtaat gaaaacgtgg ccatttgtgt gctggtggat gtttcttcgc
    33901 gcgtgaagat ggaagagtcg ttgcaggaga tggcacaagc agcggaacag gcgagccagt
    33961 caaaatcgat gttccttgcc accgtcagtc atgagctgcg aacgccgctg tatggcatta
    34021 tcggtaacct ggatctgttg caaaccaaag agttaccgaa aggcgtcgat cgtctggtga
    34081 cggcaatgaa caactcttcc agcctgttgt tgaaaattat cagcgatatt ctcgatttct
    34141 cgaagattga atcggaacag ttgaagatcg aaccgcgtga gttttcaccg cgtgaagtga
    34201 tgaaccacat caccgccaac tatttaccgc tggtggtacg caagcagtta ggcttgtact
    34261 gctttattga accggatgtg ccagtggcct taaatggcga cccgatgcgt ttacagcagg
    34321 tcatctccaa cctgttgagt aacgccataa aattcaccga taccggctgt atagttttgc
    34381 atgttcgcac tgatggcgat tatctctcta tccgtgttcg cgataccggc gtggggattc
    34441 cggcgaaaga agtggtgcgc ttgtttgatc ccttcttcca ggtcggaacg ggcgtacagc
    34501 gtaacttcca ggggaccggt ctgggtctgg cgatttgtga aaaactgatc agcatgatgg
    34561 acggcgatat ctcggtagat tcagaaccgg gaatgggtag ccagtttacc gtgcgtattc
    34621 cgttgtacgg cgctcagtac ccgcagaaaa aaggcgtgga agggttgagt ggtaaacgct
    34681 gctggctggc ggtccgcaat gcgtcgctct gtcagttcct ggaaaccagt ttgcagcgca
    34741 gcggcatcgt cgttacaaca tacgaagggc aggaaccgac tcccgaagat gtgttaatta
    34801 ctgacgaggt agtgagtaaa aaatggcagg gtagagcggt agtgaccttc tgtcgtcgcc
    34861 atattggtat tccgctggag aaagcgccag gggagtgggt acacagtgtg gctgccccgc
    34921 atgagctacc ggcattgttg gcgcgtattt atttgatcga gatggagagc gacgatcctg
    34981 ctaacgctct gccgttaacg gacaaagcgg tcagcgataa cgacgatatg atgattctgg
    35041 tcgtggatga tcatccgatt aaccggcgtc tgctggcaga tcagttggga tcgttgggct
    35101 atcaatgtaa aaccgcgaat gatggcgtcg atgcgcttaa tgtacttagc aagaatcata
    35161 ttgatatcgt gcttagcgac gtcaacatgc caaatatgga tggttaccgc ttgacgcaac
    35221 gcattcgtca gttgggactg acgttgccgg taatcggagt aactgctaat gcgttggctg
    35281 aagagaagca gcggtgtctg gagtctggta tggacagctg cctgtcgaag ccggtaacgc
    35341 tggatgtgat aaaacagacg ctaacggtat atgccgagag ggtcaggaaa tcgcgggatt
    35401 cgtagggcgg ataaggcgtt tacgccgcat ctggcattca gtgcaaatgc cagatgcgac
    35461 gctgacgcgt cttatctggc ctacagatga ttagtcttta tctgccggac ttaaggtcac
    35521 agaagagaga taattcagca gagcaatatc gttctcgaca cccagcttca tcatcgcaga
    35581 tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga tctcggtcac
    35641 caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga gacgcttgtc
    35701 accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg gggtaaattt
    35761 cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac cttgtttcag
    35821 cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt tgttgttcat
    35881 ggtcagaacg atgatcgaca ggcttgggaa atggcgcttg atgtacttga ttaaggtaat
    35941 gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat gcgcatccag
    36001 tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca
    36061 ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt catcggcaat
    36121 aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc ttgacataac
    36181 tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttttcacga atcagatgtt
    36241 ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaac acgcctttaa
    36301 gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca gtatacagcc
    36361 tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg gcatggagtt
    36421 gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt
    36481 caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg acgcacaatt
    36541 gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag ccagaggcag
    36601 taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt aatctttcat
    36661 cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata ttccgaattt
    36721 ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct tcttcctctt
    36781 caacttccgg gtcagctgcg agcattttga tatgcacaga gtagcgtgta ccaagcccat
    36841 cccgcgtttt aatgtttaaa tgaccgccca gtttacgtgc cagttgatcg ctcagccaga
    36901 atgccagcgg gtccgccttg ccatagcgat cgttttgggt ctggttgata aacgggaagt
    36961 gcaaattatc catttcatga atgcttacgc cttctccggt gtccagaatg cggaacgtca
    37021 ggcggtcttc ggaggactca tcctgatcaa cctcaagggt gatttttccc aattgcgttg
    37081 aggtcacggc atattgcatc agtagcagca aaatgcgtcg taaggcatcg cgatcgccgc
    37141 ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct ttacgcttga
    37201 tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag aacagcaccg
    37261 tctcactttt ccagctatca tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc
    37321 gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg agtttagccg
    37381 cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt ttcataaagg
    37441 tcatccgccc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt ttcttgttta
    37501 ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc gcgacctggc
    37561 tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata atcccctgat
    37621 gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa tgatcggcaa
    37681 ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg ccaagcggca
    37741 gcagtgagac tatctcttca ttgattgcac gtaaaatgcg caattcgtta ttgaccgccg
    37801 tgctggggac gttttctgta ctgcggctgg agaaatggcg gaatgtggta tagccaaata
    37861 acgccagcgc cagcaaaccg atattcagca gcagtggcag cagaatgttt tgcaacgtat
    37921 ccagcagtaa ggtgccataa ggaacctgcc agaccaggcg catatcggta gagttgagtg
    37981 ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc gtcccttctt
    38041 tctcattatc attgtttccc gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg
    38101 gcatgcccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc gttgccagat
    38161 gtcccggctg gttaaaggta gtacgcaagg taaagtaatg accgttctgc caggctaggc
    38221 ggcgcatgtt agaaaagctt tcgcgttcat cgagggcgtt ggcctgttgc aacatctctg
    38281 cacgacgtga atcaacaatg tcgctgacgg tcgattcttt aaatccggag gtgagatctt
    38341 taaggggcag ggttgagatc agcaccaggc tgttatcctg accattcagg taatacatcg
    38401 accacggtac attttctgcg ccccacaatg tatccagata agtggacatc cgttgagtca
    38461 tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg cggcgcggtt
    38521 tttccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg
    38581 gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga
    38641 cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct ttcagccagg
    38701 cgttaacggc gctttgtacc attactccca tcgtcaccag taacacaatg atcaacagta
    38761 aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt
    38821 tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg cagtataaag
    38881 ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct cccgtgatat
    38941 ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta caaataacct
    39001 acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat tatgctaatg
    39061 ttaattattt gtgaaatagt taacaagcgt tatagttttt ctgtggtagc acagaataat
    39121 gaaaagtgtg taaagaaggg taaaaaaaac cggatgcgag gcatccggtt gaaatagggg
    39181 taaacagaca ttcagaaatg aatgacggta ataaataaag ttaatgatga tagcgggagt
    39241 tattctagtt gcgagtgaag gttttgtttt gacattcagt gctgtcaaat acttaagaat
    39301 aagttattga ttttaccctt gaattattat tgcttgatgt taggtgctta tttcgccatt
    39361 ccgcaataat cttaaaaagt tcccttgtat ttacattttg aaacatctat agcgataaat
    39421 gaaacatctt aaaagtttta gtatcatatt cgtgttggat tattctgcat ttttggggag
    39481 aatggacctg ccgactgatt aatgagggtt aatcagtatg cagtggcata aaaaagcaaa
    39541 taaaggcata taacagaggg ttaataacat gaaagttaaa gtactgtccc tcctggtccc
    39601 agctctgctg gtagcaggcg cagcaaacgc tgctgaaatt tacaacaaag acggcaacaa
    39661 attagatctg tacggtaaag ttgacggcct gcactatttc tccgataacg actctaaaga
    39721 tggcgataag acctacatgc gtcttggctt caaaggtgaa actcaggtta ctgatcaact
    39781 gaccggttat ggtcagtggg aatatcagat tcaaggtaac gaacctgaaa gtgacaatag
    39841 ttcctggacc cgtgtggcat tcgctggtct gaaattccag gacgttggtt cttttgacta
    39901 tggtcgtaac tacggcgttg tttacgatgt tacttcctgg accgacgtac tgccagaatt
    39961 cggtggtgat acctacgatt ctgataactt catgcagcag cgtggtaacg gtttcgcgac
    40021 ctaccgtaac accgacttct tcggtctggt tgacggtctt gattttgctg ttcagtatca
    40081 gggtaaaaac ggtagtgctc atggcgaagg tatgaccact aatggccgtg atgatgtatt
    40141 tgagcagaac ggtgacggtg ttggtggttc tattacttac aattatgaag gctttggtat
    40201 cggtgctgca gtttccagct ccaaacgtac ctgggatcag aacaacactg gcctgatcgg
    40261 tactggcgac cgtgctgaaa cctacactgg tggtctgaaa tacgacgcta acaacatcta
    40321 cctggctgct cagtacaccc agacctacaa cgcaactcgc gtaggttccc tgggttgggc
    40381 gaacaaagca cagaacttcg aagctgttgc tcagtaccag ttcgacttcg gtctgcgtcc
    40441 gtccctggct tacctgcagt ctaaaggtaa aaacctgggt cgtggctacg acgacgaaga
    40501 tatcctgaaa tatgttgatg ttggtgcgac ctactacttc aacaaaaaca tgtccaccta
    40561 cgttgactac aaaatcaacc tgctggacga caaccgcttc acccgtgacg ctggcatcaa
    40621 cactgatgac atcgtagctc tgggtctggt ttaccagttc taatctcgat tgatatcgaa
    40681 caaagggcct gcgggccctt ttttcattgt tttcagcgta caaacacagt tttttggtgt
    40741 actcttgcga ccgttcgcat gaggataatc acgtatggaa ataagcttta cccgcgtggc
    40801 actgctggct gccgcgctct tctttgttgg ttgcgatcaa aaaacacaac ccgccaaaac
    40861 ccacgctact gaagttaccg ttcttgaagg caaaaccatg ggtaccttct ggcgtgccag
    40921 cattccgggc attgacgcca aacgcagtgc cgaacttaaa gaaaagattc agacccagct
    40981 ggacgccgac gatcagctgc tttcgaccta taaaaaagat tccgcgctga tgcgatttaa
    41041 cgactcgcaa agtttatcgc tgtggccggt aagtgaagcg atggccgata tcgtcaccac
    41101 ctcgctgcgc attggcgcaa ggaccgatgg tgcgatggat ataaccgtcg ggccgctggt
    41161 gaatctgtgg ggctttggcc cggaacaaca gccggttcaa attccgagcc aggaacagat
    41221 cgatgcgatg aaagccaaaa ccggcttaca gcacctgtcg gtcattaatc agtcgcatca
    41281 gcaatatctg caaaaagacc tgccggattt atatgtcgat ctctccactg tcggtgaagg
    41341 ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa ggtatttctc gctacctggt
    41401 gtcggtgggc ggggcgctga acagccgtgg tatgaacggt gaaggccagc cgtggcgagt
    41461 ggcgattcaa aaaccaaccg ataaagaaaa cgcagttcag gcggtggtgg atatcaacgg
    41521 ccacggtatc agcacctccg gcagctaccg caactattac gaactggacg gcaaacgcct
    41581 ttctcatgtt atcgatccgc aaaccggacg acctatcgag cataatctgg tatccgtgac
    41641 ggtgattgca ccgacggcgc tggaagccga cgcctgggat acaggtttga tggtgctcgg
    41701 gccagagaaa gccaaagagg ttgttcgccg ggaagggctg gcggtttata tgatcaccaa
    41761 agagggcgat agctttaaaa cctggatgtc gccgcagttt aaaagcttcc tggtgagcga
    41821 aaaaaattaa agcgcaagat tgttggtttt tgtgtgatgg tgaccggaca gactaaaggc
    41881 tatccttaac cagggagctg attatgaaaa acgccacacg cttaactgac gatcaacgct
    41941 ggcaatctgt cttagcacgc gacccgaacg ccgacggcga attcgttttc gccgtgcgca
    42001 ccacgggtat cttttgccgt ccgtcttgcc gcgccagaca tgccttgcgg gaaaacgttt
    42061 ctttctatac ggatgccagc gaggcgcttg ccgctggctt tcgcccctgc aaacgttgtc
    42121 agccagataa agccaatgcc cagcaacatc gccttgataa aattacccac gcctgccgac
    42181 tgctggaaca ggaaacgcct gtaacgctgg aagccttagc tgaccaggtg gcgatgagtc
    42241 cgttccattt gcatcgattg tttaaagcga ctaccggaat gacgccgaaa gcctggcaac
    42301 aggcatggcg tgctcgccgt ttacgcgact cgctggcgaa aggggagagc gtgactacat
    42361 ctattcttaa cgctggattc cccgacagca gcagttacta ccgtaaagcc gacgaaacgt
    42421 tgggcatgac ggctaaacag tttcgtcacg gaggtgaaaa tctggctgtg cgttatgcgc
    42481 tggctgattg tgaactgggc cgttgcctgg tggctgaaag cgagcggggg atttgcgcga
    42541 tattgctggg cgatgatgac gcgacattaa tcagcgagtt gcagcagatg tttcccgctg
    42601 ccgacaacgc gcctgccgat ttgacgtttc aacaacatgt gcgtgacgtg atcgccagcc
    42661 tcaatcaacg cgatacgccg ctgacgttac cgctggatat tcgcggcact gcttttcagc
    42721 aacaagtctg gcaggcactg cgcacgatac cttgcggtga aaccgtcagt tatcagcaac
    42781 tggctaacgc catcggcaaa ccgaaagcgg tacgggccgt tgccagcgcc tgtgccgcca
    42841 acaagctggc tatcgtaata ccctgtcatc gagtggtccg tggtgatggc acactttcag
    42901 gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg ccgcgaagct gagaatgagg
    42961 agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg caagagccac tggcggctgg
    43021 tgcggtaatt ttacggcgtt ttgcttttaa tgctgcggag caactgatcc gcgatattaa
    43081 tgacgttgcc agccagtcgc cgtttcgcca gatggtcacc cccgggggat atactatgtc
    43141 ggtggcgatg accaactgtg ggcatctggg ctggacgacc catcggcaag gttatctcta
    43201 ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc atgccacaaa gctttcatga
    43261 tttatgtcaa cgtgcggcta cggcggcggg ctatccagat ttccagccag atgcttgtct
    43321 tatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat caggataaag acgaaccgga
    43381 tctgcgcgcg ccgattgttt ctgtttctct gggcttaccc gcgatttttc aatttggcgg
    43441 cctgaaacga aatgatccgc taaaacgttt gttgttggaa catggcgatg tggtggtatg
    43501 gggcggtgaa tcgcggctgt tttatcatgg gattcaaccg ttgaaagcgg ggtttcatcc
    43561 actcaccacc gactgccgct acaacctgac attccgtcag gcaggtaaaa aagaataaaa
    43621 ataagaatta ttattgctgt gcgcgcgaag atgtttaaac tgcgggctgt aattcattgt
    43681 ccgggttttc tgcatggaac ttcttgtact tgtctggcgg cagtatcgct ggccatttat
    43741 cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc attggcttaa ttgcttttat
    43801 caatcagcgc cttatcgaaa cggcggatac cagtctgctg gtgttgccgg agtttctggg
    43861 gttattgctg ctgttgatgg ccgtcactct cggatcgcaa ctggcgctca ccactttggg
    43921 gcatcacttc gtttaccgac tgcgtagtga atttatcaag cgaattctcg atacccacgt
    43981 cgagcgcatt gaacaacttg gtagcgcctc gttgttggcg gggttaacca gcgatgtgcg
    44041 caatatcacc attgcttttg tgcgtctgcc ggaactggtg caggggatca tcctcactat
    44101 cggttccgcg gcgtacttgt ggatgctgtc gggcaaaatg ttgctggtaa cggctatctg
    44161 gatggcagtc accatctggg gcggttttat actggtggcg cgggtgtaca aacatatggc
    44221 gaccctgcgt gaaaccgaag acaagctgta cacggatttt caaaccgtac tcgaagggcg
    44281 caaagagctg actctgaacc gggaacgcgc cgagtatgtg tttaacaacc tctacattcc
    44341 tgatgcgcaa gagtatcgcc atcatattat ccgcgcagac accttccatc ttagtgccgt
    44401 gaactggtca aacatcatga tgctgggcgc aatcggtctg gtgttctgga tggcgaacag
    44461 tctcggttgg gctgatacca acgttgccgc gacctattcg ttgacgctgt tattcctgcg
    44521 tacgccgctg ctttcggcgg ttggtgcatt accgacgctg ctgacggcgc aggtggcgtt
    44581 taacaagctg aacaaattcg cactcgcgcc tttcaaagca gagtttccgc gcccacaggc
    44641 gtttcccaac tggcaaacgc tggagctgcg taacgtgacg tttgcttatc aggataacgc
    44701 gttttccgtt gggccgatta acctcaccat caaacgtggc gagctgctgt ttctgattgg
    44761 cggcaacggt agcggaaaat cgacgctggc gatgttgctg acgggcttgt atcagccaca
    44821 aagcggcgaa atcttgctgg atggcaaccc tgtcagcggc gaacaaccgg aagattatcg
    44881 caaactgttt tcggcagtgt ttaccgatgt ctggctgttc gatcaactgt tggggccgga
    44941 gggtcaaccc gctaacccgc aactgattga gaagtggctg gtgcagctga aaatggctca
    45001 taagcttgag ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag ggcagaaaaa
    45061 acgcgtcgcg ctgttgctgg cgctggcaga agaacgcgat attatcctgc tggatgaatg
    45121 ggcggcggat caggatccac acttccgtcg tgagttttat caggtgttgc tgccgctgat
    45181 gcaggagatg ggtaaaacta ttttcgccat cagtcatgat gatcattact ttatccacgc
    45241 cgaccgcctg ctggaaatgc gcaacgggct acttagcgag ctgacaggcg aagagcgcga
    45301 tgctgcttcg cgtgatgccg ttgcccggac ggcataatat ttcgacgctg acctggcgga
    45361 ctgctgctgc caggtcaata ttctaaaaat cccttcgttt ctaataaccc tataattaat
    45421 caacgaaatt ataatgtttc taaaaataga atataattta taaacattat ttaaatgttg
    45481 ttacttaagt gttaccgttg atgccgcgca aatctcacct gcaataaagc gactaaaagt
    45541 aaggcattaa caagatgaaa aaagtgactg ccatgctctt ctcgatggcc gtgggcctta
    45601 atgccgtttc gatggcggca aaagcgaaag cgtccgagga gcaggaaact gatgtactgt
    45661 tgattggcgg cggcattatg agcgccacgt tggggaccta tttacgcgag ctggagcctg
    45721 aatggtcgat gaccatggtg gagcgcctgg agggtgtcgc gcaggagagt tcgaacggct
    45781 ggaacaacgc cggaaccggg cattctgcac tgatggaact caattacact ccgcaaaacg
    45841 ccgatggcag catcagtatt gaaaaagcag tcgccattaa cgaagcattc cagatttccc
    45901 gccagttctg ggcgcaccag gtcgagcgcg gcgtgctgcg tactccgcgt tcatttatca
    45961 ataccgttcc gcatatgagc tttgtctggg gcgaggataa cgtcaatttc ctgcgcgccc
    46021 gttacgccgc gttacaacaa agctcgctgt ttcgtggtat gcgttactct gaagatcacg
    46081 cgcagatcaa agagtgggca ccgttagtga tggaagggcg cgatccgcaa cagaaagtgg
    46141 cagccacgcg tacggaaatc ggtactgatg tgaactatgg cgagatcacc cgccagttaa
    46201 ttgcttcctt gcagaagaaa tctaattttt cgctgcaact cagcagcgaa gtccgcgccc
    46261 taaagcgtaa tgacgataac acctggaccg tcactgttgc cgatctgaaa aacggcactg
    46321 cacagaacat ccgcgctaaa tttgtcttta tcggcgcggg cggtgcggcg ctgaagttgt
    46381 tacaggaatc gggtattccg gaagcgaaag actacgccgg tttcccggtg ggcggacagt
    46441 tccttgtttc ggaaaacccg gacgtggtta atcaccatct ggcgaaggtt tacggtaaag
    46501 catccgttgg cgcaccaccg atgtcggttc cgcatatcga tacccgtgtt ctggacggta
    46561 aacgcgtagt gctgtttggg ccatttgcca ccttctcaac caaattcctc aaaaacggtt
    46621 cattgtggga tctgatgagt tccaccacca cctctaacgt gatgccgatg atgcacgttg
    46681 ggctggataa tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg agtgaagagg
    46741 atcgttttga agcgttgaaa gagtactatc cgcaggcgaa aaaagaggac tggcgtttgt
    46801 ggcaagcggg gcagcgtgtg cagattatca agcgtgatgc cgagaaaggt ggcgtactgc
    46861 gtctgggtac cgaagtggtc agcgaccagc aagggactat tgccgcgctg ctgggggcat
    46921 cgccaggtgc gtcaaccgcc gcgccgatta tgttgaatct gctggaaaaa gtatttggcg
    46981 atcgcgtttc cagcccgcaa tggcaggcaa cgttgaaggc gatcgttccg tcttatggac
    47041 gcaagctgaa cggtgatgta gcggcaacgg agcgcgagtt gcagtacacc agcgaagtgc
    47101 tggggctgaa atacgacaaa ccgcaagcag cagatagtac gccgaaaccg cagttgaaac
    47161 cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta
//
LOCUS       DAOTRK010000015        46833 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.015, whole genome shotgun sequence.
ACCESSION   DAOTRK010000015 DAOTRK010000000
VERSION     DAOTRK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.015"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..829)
                     /gene="pdxK"
                     /locus_tag="QIE41_000947"
                     /gene_synonym="thiJ"
     CDS             complement(<1..829)
                     /gene="pdxK"
                     /locus_tag="QIE41_000947"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDS3529861.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     "
     gene            934..1302
                     /locus_tag="QIE41_000948"
     CDS             934..1302
                     /locus_tag="QIE41_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDS3529862.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVSAQ"
     gene            complement(1305..2216)
                     /gene="cysM"
                     /locus_tag="QIE41_000949"
     CDS             complement(1305..2216)
                     /gene="cysM"
                     /locus_tag="QIE41_000949"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDS3529863.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2350..3447)
                     /gene="cysA"
                     /locus_tag="QIE41_000950"
     CDS             complement(2350..3447)
                     /gene="cysA"
                     /locus_tag="QIE41_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDS3529864.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEALTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3437..4312)
                     /gene="cysW"
                     /locus_tag="QIE41_000951"
     CDS             complement(3437..4312)
                     /gene="cysW"
                     /locus_tag="QIE41_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDS3529865.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4312..5145)
                     /gene="cysT"
                     /locus_tag="QIE41_000952"
     CDS             complement(4312..5145)
                     /gene="cysT"
                     /locus_tag="QIE41_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDS3529866.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5145..6161)
                     /gene="cysP"
                     /locus_tag="QIE41_000953"
     CDS             complement(5145..6161)
                     /gene="cysP"
                     /locus_tag="QIE41_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDS3529867.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6332..7123)
                     /gene="ucpA"
                     /locus_tag="QIE41_000954"
     CDS             complement(6332..7123)
                     /gene="ucpA"
                     /locus_tag="QIE41_000954"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDS3529868.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7252..8109)
                     /gene="murR"
                     /locus_tag="QIE41_000955"
     CDS             complement(7252..8109)
                     /gene="murR"
                     /locus_tag="QIE41_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDS3529869.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            8273..9169
                     /gene="murQ"
                     /locus_tag="QIE41_000956"
     CDS             8273..9169
                     /gene="murQ"
                     /locus_tag="QIE41_000956"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDS3529870.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALVACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9173..10597
                     /gene="murP"
                     /locus_tag="QIE41_000957"
     CDS             9173..10597
                     /gene="murP"
                     /locus_tag="QIE41_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDS3529871.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            10602..11903
                     /gene="pbp4b"
                     /locus_tag="QIE41_000958"
     CDS             10602..11903
                     /gene="pbp4b"
                     /locus_tag="QIE41_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDS3529872.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(11961..12860)
                     /gene="yfeX"
                     /locus_tag="QIE41_000959"
     CDS             complement(11961..12860)
                     /gene="yfeX"
                     /locus_tag="QIE41_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDS3529873.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12956..13531)
                     /locus_tag="QIE41_000960"
     CDS             complement(12956..13531)
                     /locus_tag="QIE41_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDS3529874.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13592..14041)
                     /locus_tag="QIE41_000961"
     CDS             complement(13592..14041)
                     /locus_tag="QIE41_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDS3529875.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14028..14453)
                     /locus_tag="QIE41_000962"
     CDS             complement(14028..14453)
                     /locus_tag="QIE41_000962"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDS3529876.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14667..15536
                     /gene="amiA"
                     /locus_tag="QIE41_000963"
     CDS             14667..15536
                     /gene="amiA"
                     /locus_tag="QIE41_000963"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDS3529877.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            15540..16439
                     /gene="hemF"
                     /locus_tag="QIE41_000964"
     CDS             15540..16439
                     /gene="hemF"
                     /locus_tag="QIE41_000964"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDS3529878.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYHPEDGSPEAALSEFIKVRDWV"
     gene            complement(16445..17497)
                     /gene="eutR"
                     /locus_tag="QIE41_000965"
     CDS             complement(16445..17497)
                     /gene="eutR"
                     /locus_tag="QIE41_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDS3529879.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSMTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17543..18043)
                     /gene="eutK"
                     /locus_tag="QIE41_000966"
     CDS             complement(17543..18043)
                     /gene="eutK"
                     /locus_tag="QIE41_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDS3529880.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVVESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18056..18715)
                     /gene="eutL"
                     /locus_tag="QIE41_000967"
     CDS             complement(18056..18715)
                     /gene="eutL"
                     /locus_tag="QIE41_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDS3529881.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18725..19612)
                     /gene="eutC"
                     /locus_tag="QIE41_000968"
     CDS             complement(18725..19612)
                     /gene="eutC"
                     /locus_tag="QIE41_000968"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDS3529882.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19633..20994)
                     /gene="eutB"
                     /locus_tag="QIE41_000969"
     CDS             complement(19633..20994)
                     /gene="eutB"
                     /locus_tag="QIE41_000969"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDS3529883.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(21006..22409)
                     /gene="eutA"
                     /locus_tag="QIE41_000970"
     CDS             complement(21006..22409)
                     /gene="eutA"
                     /locus_tag="QIE41_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDS3529884.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPNSDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22406..23632)
                     /gene="eutH"
                     /locus_tag="QIE41_000971"
     CDS             complement(22406..23632)
                     /gene="eutH"
                     /locus_tag="QIE41_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDS3529885.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEVEAQS"
     gene            complement(24148..25335)
                     /gene="eutG"
                     /locus_tag="QIE41_000972"
     CDS             complement(24148..25335)
                     /gene="eutG"
                     /locus_tag="QIE41_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDS3529886.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25325..26161)
                     /gene="eutJ"
                     /locus_tag="QIE41_000973"
     CDS             complement(25325..26161)
                     /gene="eutJ"
                     /locus_tag="QIE41_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDS3529887.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            complement(26172..27575)
                     /locus_tag="QIE41_000974"
     CDS             complement(26172..27575)
                     /locus_tag="QIE41_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDS3529888.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGSVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27587..27874)
                     /gene="eutN"
                     /locus_tag="QIE41_000975"
     CDS             complement(27587..27874)
                     /gene="eutN"
                     /locus_tag="QIE41_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDS3529889.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIIDEVVSGGQVIFHK"
     gene            complement(27981..28274)
                     /gene="eutM"
                     /locus_tag="QIE41_000976"
     CDS             complement(27981..28274)
                     /gene="eutM"
                     /locus_tag="QIE41_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDS3529890.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28313..29329)
                     /gene="pta"
                     /locus_tag="QIE41_000977"
     CDS             complement(28313..29329)
                     /gene="pta"
                     /locus_tag="QIE41_000977"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDS3529891.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWQAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPQLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29326..30129)
                     /gene="eutT"
                     /locus_tag="QIE41_000978"
     CDS             complement(29326..30129)
                     /gene="eutT"
                     /locus_tag="QIE41_000978"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDS3529892.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGD
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(30126..30827)
                     /gene="eutQ"
                     /locus_tag="QIE41_000979"
     CDS             complement(30126..30827)
                     /gene="eutQ"
                     /locus_tag="QIE41_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDS3529893.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESTPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30802..31281)
                     /gene="eutP"
                     /locus_tag="QIE41_000980"
     CDS             complement(30802..31281)
                     /gene="eutP"
                     /locus_tag="QIE41_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDS3529894.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGDYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31294..31629)
                     /gene="eutS"
                     /locus_tag="QIE41_000981"
     CDS             complement(31294..31629)
                     /gene="eutS"
                     /locus_tag="QIE41_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDS3529895.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31922..34201)
                     /gene="maeB"
                     /locus_tag="QIE41_000982"
     CDS             complement(31922..34201)
                     /gene="maeB"
                     /locus_tag="QIE41_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDS3529896.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRSDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34490..35440
                     /gene="tal"
                     /locus_tag="QIE41_000983"
     CDS             34490..35440
                     /gene="tal"
                     /locus_tag="QIE41_000983"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDS3529897.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGRKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35460..37463
                     /gene="tkt"
                     /locus_tag="QIE41_000984"
     CDS             35460..37463
                     /gene="tkt"
                     /locus_tag="QIE41_000984"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDS3529898.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKVHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37566..38609)
                     /locus_tag="QIE41_000985"
     CDS             complement(37566..38609)
                     /locus_tag="QIE41_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDS3529899.1"
                     /translation="MRYRIFLLFFFALLQTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLNGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38735..39310)
                     /gene="nudK"
                     /locus_tag="QIE41_000986"
     CDS             complement(38735..39310)
                     /gene="nudK"
                     /locus_tag="QIE41_000986"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDS3529900.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(39378..41357)
                     /gene="aegA"
                     /locus_tag="QIE41_000987"
     CDS             complement(39378..41357)
                     /gene="aegA"
                     /locus_tag="QIE41_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDS3529901.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41563..43263
                     /gene="narQ"
                     /locus_tag="QIE41_000988"
     CDS             41563..43263
                     /gene="narQ"
                     /locus_tag="QIE41_000988"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDS3529902.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43427..46540
                     /gene="acrD"
                     /locus_tag="QIE41_000989"
     CDS             43427..46540
                     /gene="acrD"
                     /locus_tag="QIE41_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDS3529903.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46639..46698)
                     /gene="ypfM"
                     /locus_tag="QIE41_000990"
     CDS             complement(46639..46698)
                     /gene="ypfM"
                     /locus_tag="QIE41_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDS3529904.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10677 a        13210 c        12201 g        10745 t
ORIGIN      
        1 aaatcaattc atcgctctca tgctgctggg tgtagcgcat cacttccagt acgcgcaacc
       61 ccgcacggtg caccgcatcg tttaacgcct tccctttcag caaaccactg ataagctgag
      121 cacaaaacag gtcgccagtc cctttcaggt cggtttttac ccgtgaatgg gaaatgacat
      181 tcacgctgtc ggcactgacc accaccacct gcatctcctg attttcttca ttaccggagg
      241 cgctggtaat caccacccat tttaatgtgt ctgaaagcag actttttgcg gcagcaatgg
      301 cactgtcgag atcgcggcaa tttttaccgg tcaggatttc cagctcaaag atattggggg
      361 taattccctg cgccagcggc agtaaatatt gtcgatacgc ctcgggaagg tcaggtttga
      421 cataaattcc gctatcaata tcgccaatca ctggatcgac catgatcaat aggtcaggat
      481 gatctttgcg tagcgcagtc agccactcgg caaggatttt gatttgcgat gccgttccca
      541 tatagcccgt ggttacagca cgaagttggc gcaacgcatc acgctcctga agcgcacgca
      601 aatagccgct aaaccattcg tccggaatcg caccaccgta gaaagtgtca taatgcggtg
      661 tattgctcag caataccgtc ggcacggcaa agacattcag gccgttctgt ttgatagcag
      721 gcacggcaat gctgttgcct acgctgccgt acactacctg cgactgcacg gcgacgatat
      781 ctgcctgcaa tgccctgctc ttatcgttaa acaacaacaa actactcatt taattttttc
      841 tccttgccga tgatcctcat cgtattccaa ccgaaacttt acctgattct ggcagtcaaa
      901 tcggctatca caaaacaagg ataaggtaat tcaatgaaga aaatcatttg tctggtcatt
      961 acgctgctaa tgacactccc cgtttacgcg aagttaactg cccatgaaga agcccgtatc
     1021 aacgccatgc tggagggatt agcacagaaa aaggatttga tatttgtgcg taacggcgat
     1081 gaacacacct gtgatgaagc ggtttcccac ctgcgtctga agctcggcaa tacccgtaac
     1141 cgcattgaca ctgccgaaca atttattgat aaggttgctt cgtcatcatc gattaccggg
     1201 aagccgtata tcgtgaagat gcccggtaag agcgatgaaa atgcgcaacc ttttttacat
     1261 gcgctgattg cgcagacgga taaaacggtg tctgcgcaat aatcttaaat ccccgccccc
     1321 tggctaaagt gttcttcccc aaacacgccg gtggagaggt agcgatcgcc gcgatcgcag
     1381 atgatcgcca caaccaccgc gccggggtta gcttttgcca cgcgcagtgc tccggcaacc
     1441 gcgccgccgg agctaacgcc acagaatatt ccttcccgca ccgccagttc acgcatggtg
     1501 ttttctgcat cgcgctgatg aatatccagc acctcatcca ccagcgaagc gttgaaaatc
     1561 cccggcagat attccgcagg ccagcggcga atgccgggaa tgctgctgcc ctcttccggt
     1621 tgcaggccga caatggtcac cggtttggat tgttcgcgca taaagcgtga cacgccggtg
     1681 atggtgccgg ttgttcccat gctggagaca aaatgggtaa tgcgcccgcc agtttgctgc
     1741 cagatttccg gcccggtggt ggtgtaatgc gcataagggt tatcgggatt attgaactga
     1801 tcgagcagct ttccttcacc acgattcgcc atctccagcg ccagatcgcg cgcaccttcc
     1861 atgccctgct ctttggtgac aagaatcagt tccgcaccat aagcacgcat cgccgcacgg
     1921 cgttcctggc tcatgttgtc gggcatcagc aatttcatgc gatagccttt aagcgcggca
     1981 atcattgcca gcgcaatgcc ggtgttacca ctggtggctt cgatcagcac gtcacccggt
     2041 ttaatttccc cgcgcttttc cgcctcgacg atcatcgaaa gtgccgcacg atctttcacc
     2101 gaacctgccg ggttattgcc ttccagtttc aaccagactt cactgccgtt attcggcccc
     2161 attcgctgca acttcaccag aggcgtattg ccaattgttt gttctaatgt actcacgatc
     2221 tctatccata cgtggtgttg cctgatgcga cgcttgcgcg tcttatcagg cctacaggtt
     2281 acaaaccttg ccattaaaaa gcccggatcg cgggaagcgc ctccgggcgt ttaacattca
     2341 ctcaacctat caggcgcttt gtgcgagagc aagttcctca tcgcgggttt caatacgctc
     2401 gtcgccgtta tacagccgcg catgttgcag accaacgaat aaacgctcgc cacgttgcgg
     2461 ggcgtcgtcg ccgtgcatca cgaccgtcag cgcttcgttg taccacccca gcggctgcac
     2521 gactaattgg gtgtagtggc ctttcgggct ggcttccagt acctgtaccg gcagcggcga
     2581 atcgaggctg gtacggcggc tgatatccac ttcccacggg cgcaggaaga gatccaccgg
     2641 cccctgatac gcaggtgtat agcccagcgg ccagcgatgt gcgccaacat ggaactgccc
     2701 gccgcgaatg gttccctgca ggcggttcac ttcgcccata aattcgagca caaagcgggt
     2761 cgctggttcg cgccatacct gatccggcgc atcagcctgc tcgatattgc cctggctcat
     2821 caccactaca cgatcggcta cttcggtcgc ttcttcctgg tcatgggtca cgaatacgct
     2881 ggtaaatttc agttcttcat gcagttgacg caaccagcga cgcagctctt tacgcacctg
     2941 cgcatccagc gcgccaaacg gttcatcaag cagcagaatt tgcggttcca cagcgagcgc
     3001 acgcgccagc gccacgcgct gtttctgccc gccggaaagc tgtgccggat aacgatccgc
     3061 cagatgcgca agctgcacca tctccagcaa ttttgtcact ttcgctttga tggctgcggc
     3121 attcgggcgc tcgcgacgcg gcagcaccgt caggccgaaa gcgatattgt cgaacaccgt
     3181 catatggcgg aacagcgcat agtgctggaa cacgaaacca actttacgat cgcgcgcatg
     3241 caggcggctg acgtcggtgc cgtggaagcg aatatgcccg ctggtttgat gctccagccc
     3301 ggcgataatg cgcagcagcg tggttttccc ggaaccggac ggccccagca acgcgaccat
     3361 ctgaccagaa ggaatatcca gtgagatatc gttcagcacc tgggtgcgac caaaagactt
     3421 cttaatattg gcaatctcaa tgctcatgat gttcctcctg ctgcgcgcgt ttttcctgat
     3481 tctccagacg ccactgcaac atactcttta aaaacagggt gataatcgcc atcagcgtca
     3541 acaacgcggc agcggtaaag gagccgacgg tgttgtagtc ttgctccagc aattcaatct
     3601 gtaacggcag cgaaagtgtt tcgccgcgaa tcgagccgga aaccaccgac accgcaccaa
     3661 actcgccaat cgcgcgggcg ttggtcagca ccacgccata aagcagcgcc cagcggatgt
     3721 tcggcagcgt gacgcggcga aacatctgcc agccagatgc gcccagcaaa atcgccgctt
     3781 cgtcttcctg gctgccctgg cttaacatca ccggcaccag ctcacgcacc acaaacggac
     3841 acgtcacgaa gatggtgacc agcaccattc ccggccagga gaacataatt tgcagattat
     3901 gttcatccag ccagccgccg agcgggccgt tagagccgta aaacagcaga tacaccagac
     3961 cggcaaccac cggcgacacg gcaaacggaa tgtccagcag cgtcagcagc aactggcgtc
     4021 cggggaagtt aaagcgcgtc accagccatg ccagcagaat gccgaacacc aggttcaccg
     4081 gcacggcaat cagcgcaatc atcaccgtca gccagatggc gtgcagcatg tccggatcgg
     4141 ccagattctg taaaactggc atcagccctt tactgaacgc ctgcacaaag atgtaaatca
     4201 tcggcaccag caggatgaac gccgaaacca gcatcccgat gccaatcaga aaccatttgc
     4261 cccagttaat cgggcgcgcg tcataacgct tcaactgtgt cacttccgcc attaatgacc
     4321 taccacacgc cgaccaaagc gactttgcag agtgttaatt gagaacagca gcagcaggga
     4381 tgccgcgagg atcaccgaag caatcgcgct cgccgccggg taatcaaact cctgtaagcg
     4441 cacaaaaatc atcagcgacg tcacttccgt cttccaggcg atgttcccgg cgataaaaat
     4501 caccgcgcca aactcaccaa gactacgagt aaacgacagc gccacgcccg ccaccagcgc
     4561 cggagaaagt tccggcagca ccactttgca gaaactctgc cagcgcgttg cgccaagcgt
     4621 ttctgccgct tcttcatatt ccgggcctaa ctcttccagc accggctgca cggtacgtac
     4681 cacaaacgga atgctggtaa aggccatcgc caccgcaatc cccagccagg tataggtgac
     4741 tttgatatca aacttcgcca gccattcacc gtaaaaaccg tttacagaaa agagcgaggc
     4801 cagcgttaaa ccggcgactg ccgttggcag cgcaaagggt aaatccatca gcgcatcaag
     4861 cagcgtgcgg cccggaaagc gataacgggt taagatccac gccatcagca ggccgaaaac
     4921 gccgttaaaa attgatgcca caaacgccga cagcagcgtc actttgtagg ccgcgaccac
     4981 ttgtgggttg gtgatcacct cccagtactg cgcccagctc atctgagcca gctgcatcac
     5041 cagcgcggag agcggcagca gcaaaataag gcacacaaac agcagactgg tgccgaggct
     5101 taaggtaaag cccggcagca cgcgtcttga tgaaactgca aacattaatt acgccccgcc
     5161 gctaacagct tgtctaactc gccaccgctg gtgaaatggg ttttcatcac ttccggccag
     5221 gagccaaatt tgtcttccac gcggaacagc tcggtctgcg ggaatttatc tttcagtttg
     5281 tccatgactt ccgggttatt tacgcggtag taatagtcgg tgatgatggt ctgcgcctgc
     5341 gggctgtaga gccagttcag gtaagctttt gctgcttttt ccgtaccgtt ggcctgcacg
     5401 tttttatcga cccacgccac cgggaattcc gccagaatat tggttttcgg aatcaccact
     5461 tcaaagcctt gcgcttcata ctgtttgcga atgttgttca cttccgactc gaagctgatc
     5521 agcacatcgc ccaggccgcg ctcggcaaaa gtggtggtcg cgccacgacc gccagtatcg
     5581 aacacttcaa cgtttttcag gaactgggtc ataaactgtt cggttttggc tttgtcgcca
     5641 ccgtcagctt tatccgctgc gccccatgcc gccagatagg tgtaacgtgc gttacccgac
     5701 gttttcgggt tcgggaaaat cagctttacg tcggagcgca ccagatcgtt ccagtcgtgg
     5761 atattcttcg ggttaccctt acgcaccagg aagcccatgg tggagtagaa cggcgagctg
     5821 ttattcggca ggcgcgtttg ccagtcggcc gggatcagct tgcctttgtc gtgcaggatt
     5881 tgtacgtcag tcacctggtt ataagtgaca acgtcggctt ttaagccctg caaaatcgcc
     5941 agcgcctgtt ttgatgaccc ggcatgagat tgttttatcg tcagtttgtc gccgccgtta
     6001 tctttggccc attgttgctc aaacggcgga ttcagggcgg caaacagctc gcgggagacg
     6061 tcataagaac tgttcagcag ttccgttgcc tgtacatggc ccgccagcag cagagaagcg
     6121 accagcgcga gtgagttctt tttcagtaag ttaacggcca ttgcgcaccc ttataaattt
     6181 aatgactttc taatagccat catatttata acggacgtga aaggagtaac ggttttatat
     6241 accgtttggt gatttggaag ttgaaaaggg aataagaacc gggttcaaga cgaaatcctc
     6301 cccacaaatg cagggaggaa acagaggtga atcagatgcc gacgctaacc gtctccggca
     6361 gtgtgctgcc gccatcaatc acattctgtg taccggttaa ataacttgat tcatccgatg
     6421 cgaggaaggc tgccagttcg ccgacttcca gcggatcggc gaggcgacgc atcgggattg
     6481 cttttgccat ttcagtcagc accgattctg gatcttccgg gttcgactgg cgggcaatgc
     6541 tttccgccat tggcgtgcgt acgtatcccg ggcagatggc gttaacgcga ataccagact
     6601 gcgcgtactc caccgccagc gatttagtca ggccaacaat cgccgctttc gttaaggcat
     6661 acgccgtttc acccggatcg gccaccatat cgccagtgac tgaagacatc atcacaatgc
     6721 gaccatcttt gcgggcaatc atctccggca gcaccgcctt cgtgacgttc catacgcctt
     6781 taatattgat atcaatatgg aaatcgcgat cttcatcgct catatcgagg aaactgccca
     6841 gacgacaaac gcctgcgtta ttcaccagga tatcaatgcg cccttctttt tccttcgcgc
     6901 gcttgatagc tgcggcgacc gacgccgggt cacgcacatc ggcgacaacc gccgtacagc
     6961 gatgaccacg accacacagt tcgtccgcca gcttttcgat ctcaggggag atatccagca
     7021 agattaggtt tgcgccatga cgcgcaaaag ttctggcaat tccttcgcca attccctgca
     7081 atgcgcccgt aatcagtgct gtcttgcccg tgagtttacc cattttaatg ctctccttgt
     7141 taatacagag cgttacactc cattccctct gaaaatacag gataaacaaa atattgctgc
     7201 ggatcagctc actaattcag gaaaatctca atccagtcgc tgctttatgc atcatttcag
     7261 gcgttgcgtt agctcactgc tgcgctcaat cattttcaat gactccacat cattgagttg
     7321 cacaaggcca acaaacagta aatcggtaac agaattttgc gccgtgcggg tggacatcga
     7381 cgagctgcgc cattcggttt cgccagaaac ggtatcgagg gtgtaatgcg ccagccgccg
     7441 cagcggggaa tcggttaacg aggtaatggc aatcaccgtt gccccctgct ttcgtgctgc
     7501 ctcggcgcac aagacaatct cttttttact gccgctgtaa gagatagcga tttgtacatc
     7561 gccttttttc agcgcctggg aaacggtggc ttgtacatgc gtgtcagctt cacaggccac
     7621 acgataacca attttcatca gtttgaatga aagatcgcgc cccaccagcg ccgacccacc
     7681 caggcccgtt atctggataa acggtgcttt actgatgacc tcaatgattt tttgcaaccg
     7741 cgcgtagtcg aacaacgcac aggtttgctc cagcgccagc tctttttcgc gattcaattt
     7801 gcgggcaatc acttccagcg aatcatcact ggtaatcgaa ctgtgcaggt gcagcgccgt
     7861 ggcattcgtt ttctcccggc tagcactgta ttcgccaatt aacgccatac gcaattcagt
     7921 aaatccttgc gcaccgagct tctgggcaaa cttcacaata ctcgactggc tgatgccgag
     7981 ctgtttcgcc atcttgcgcg aagagacgga tttcagctca ctcacgttgg cccgcagaaa
     8041 gtcagcaatt ttttgctcgt tcccggtaaa ttcactttcc gcattgcgta ttttcgtcag
     8101 gtacaacatc ttccactctc ctgggctggc agcaaaaatt ccgcttaagt tcttatgacg
     8161 ctcttcacac tctgcgagtg tagactcaat gattccttta agtctgtcaa ttcgggatat
     8221 atgattccat acatctgcaa ttatttgatc gtaaatagta aggtcaccac cgatgcaact
     8281 tgaaaagatg attaccgaag gctcgaacgc cgcctcggct gaaattgacc gcgtatcaac
     8341 gctggaaatg tgccggatta tcaacgatga agataaaacc gtaccactcg ccgttgagcg
     8401 cgtactgccg gatatcgccg cggcgatcga tgttatccac gcccaggtaa gcggcggcgg
     8461 gcgtctgata tatctcggtg cgggaacatc cggtcgtctg gggattctgg atgccagcga
     8521 atgtccgccc acctacggcg ttaaaccggg tctggtggtt ggtttgattg ctggaggcga
     8581 atatgccatt cagcacgcgg tggaaggcgc tgaagatagc cgggaaggtg gtgttaacga
     8641 tctgaaaaac atcggcttaa ccgcgcagga tgtggtggtt ggcattgcgg ccagtggtcg
     8701 cacgccgtat gtaattgccg gactggaata cgcacgccag ctcggctgcc gcacagtggg
     8761 aatttcctgt aatcccggga gcgccgtttc aaccaccgct gagtttgcca ttacgccagt
     8821 tgttggggcc gaagtggtca caggttcttc gcgaatgaaa gcaggtacgg cgcagaaact
     8881 ggtgctcaat atgctctcca ccgggctgat gattaaatcc ggcaaagtgt tcggcaacct
     8941 gatggtcgat gtggtcgcca ccaacgaaaa attgcatgtg cgccaggtca atatcgtcaa
     9001 aaacgctacc ggatgtagcg cagagcaagc ggaagcggca ttggttgcct gcgagcgcaa
     9061 ctgtaaaacg gccattgtga tggtgctgaa aaatctcgat gccgcagaag ctaaaaaacg
     9121 tcttgatcaa cacggcggct ttattcgtca ggttttagac aaggaataac ccatggccaa
     9181 agagatcagc agtgaacttc tgaacaccat tcttacccgt gtcggcggac cgggaaatat
     9241 cgccagttgt ggtaactgta tgacgcgcct gcgtctgggt gtacatgaca gttcactggt
     9301 tgatcccaat atcaaaacgc tggaaggcgt gaagggcgtc attttgacca gcgatcaggt
     9361 gcaggtggtt tttgggccgg gcaaagcgca tcgtgccgca aaagccatga gcgagctgct
     9421 gggagaagca ccggtacagg atgccgcaga aatcgccgcc cagaacaaac gtcagttaaa
     9481 agccaaacaa acctccggcg tgcaacaatt tctcgccaaa ttcgccacca tcttcacgcc
     9541 gctgatcccc ggttttattg ccgccggtct gctgctgggg atagcgacgt taattgccac
     9601 ggtgatgcac gttccggcag atgctcaggg cacgctaccc gacgcgctga attttatgaa
     9661 ggtgttcagc aaaggtttgt tcaccttcct ggtgattctg gtgggctata acgccgccca
     9721 ggcatttggc ggcacgggcg taaatggcgc gattatcgcc gcgctctttt tgctcggtta
     9781 taacccaact gcaaccaccg gttactacgc cggttttcac gatttctttg gtctgcccat
     9841 cgatccgcgc ggcaatatta tcggcgtgtt gattgccgcg tgggcctgcg cccgcatcga
     9901 aggcatggtg cgccgcttta tgccggacga tctcgacatg ctgttgacct cgttaatcac
     9961 cctgctgatc accgctacgc tggcctacct gatcattatg ccgctgggcg gctggctgtt
    10021 tgaaggcatg tcgtggctgt ttatgcacct gaacagtaat ccgctcggtt gtgcggtttt
    10081 agccgggctg ttcctgatcg ccgtggtgtt tggcgtgcat cagggcttta ttcctgttta
    10141 cctcgcgtta atggacagtc agggattcaa cagcttattt cccatccttt cgatggcagg
    10201 cgcgggccag gtgggcgcgg cgctggcact ctactggcgg gcgcaaccgc acagtgcatt
    10261 acgcagtcag gtacgcgggg cgattattcc cggcctgctg ggcgttggcg aaccgttgat
    10321 ttatggcgtc accctgcccc gcatgaaacc gtttattacc gcctgtttag gcggcgcggc
    10381 gggcggtttg tttatcggat tgatagcctg gtggggtctg ccgatgggct taaacagcgc
    10441 ctttgggccg tctggtctgg tggcgctgcc gctgatgact tccgcacaag gcatccttcc
    10501 ggcaatggcg gtttatgcag gcgggattct ggtggcatgg gtttgcgggt ttattttcac
    10561 cacgctcttt ggctgccgta acgtcaatct ggactgataa tatgaaacgg acaatgctct
    10621 atctttctct gctggctgtc agctgtagcg tcagcgccgc aaaataccct gttctgacag
    10681 aaagttcgcc agagaaagca gggttcaacg tcgaacggct taaccagatg gatcgctgga
    10741 taagccagca ggtggatgcc ggttatcccg gcgtaaacct gctgattatt aaagataatc
    10801 agattgtgta tcgcaaagcc tggggagcgg cgaaaaagta cgatggcagc gtattgatgg
    10861 aacagcctgt caaagccacc accgggacgc tgtatgacct ggcttccaat accaagatgt
    10921 acgccactaa ctttgctctg caaaagctaa tgagcgaagg caagctgcat cctgacgatc
    10981 ggattgcgaa atatattccg ggatttgccg atagtccaaa tgacaccatc aaaggcaaaa
    11041 acacgctgcg gatttctgac ctgctgcatc acagcggcgg tttcccggcg gacccacaat
    11101 atccgaataa agcggtcgcg ggcgcgttat attcccagga taaaggccag acgctggaga
    11161 tgatcaagcg cacgccgctg gagtatcagc ccggcagcaa gcatatctac agcgatgtcg
    11221 attatatgct gcttggattt atcgtcgagt cggttaccgg tcagccactt gaccgctatg
    11281 ttgaagagtc gatttatcgc ccgctcggcc tgacgcatac ggtgtttaat ccgctactga
    11341 aaggctttaa accgcaacaa attgccgcca cggaactcaa cggcaatacc cgcgatggcg
    11401 tgatccattt cccgaatatc cgcacctcca ctctctgggg tcaggtgcac gatgaaaaag
    11461 ccttttattc gatgggcggc gtttccgggc atgcagggtt gttttccaat accggcgata
    11521 ttgcggtgtt aatgcaaacg atgctgaacg gcggcggcta tggcgatgtg cagttgttca
    11581 atgcagaaac ggtgaagatg ttcaccacca gctctaagga agatgccact tttggccttg
    11641 gctggcgcgt gaatggtaat gccaccatga cgccgacgtt tggcacgctg gcaagcccgc
    11701 aaacctacgg tcacactggc tggaccggga cggtgaccgt tatcgatccg gtgaatcata
    11761 tggcgattgt gatgttaagc aacaagccac attcgccagt tgccgatcca caaaaaaatc
    11821 ccaatatgtt cgaaagcggt cagctaccga ttgcaactta tggttgggta gttgatcagg
    11881 tgtatgcggc gttaaagcag taataaaaag gccagtcagt tgtgatactg gctggcctta
    11941 aatcatcgaa tcagaaacga ttacaacgcc atcaacttgt ccagcgacgg cgcgaagtaa
    12001 tagccgccag tgaccggttt ggtgaaacgc aacatcgcat cacgtttgcc atcggtatcg
    12061 ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta ggcgcagaag
    12121 tacagaccgt gagtgccact ggcggtgccg tacggcaggc tctggcgaac aatcttcagc
    12181 cctttgccat cttctttcag atcaacgcgg gtcaggtgag aggtttccgg acgttcgtcg
    12241 ccgtcaatct cttcgttggc ctctttagtg cgcccgatca tcatctcttg atcgtgaacg
    12301 ctcatacggt tgagttgctt caggttgtgc tcccaacgct gcacaaacac atagctgccg
    12361 cccgcatcca cgccgtcttt gataaccgcc acttcgcggc gcgtctcttc acccgccggg
    12421 ttttctgtac cgtcaacgaa gccgctcagg tcacgctctt caacccaacg gaagccgtgg
    12481 atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg agcgacagag
    12541 aagtttacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg ggtcgtcggc
    12601 gcaaggcctt taccgtagcc cggaaaatct ttcagctctt cggccccaac gccgccgctc
    12661 agagcgcgcc aggtgttgtt accaaaggca accaccgcac caagatgcgc atccgggaat
    12721 ttcgcttcaa aagttgccag tttatcggca aatgttttac tggccgcacg cagggcgtca
    12781 acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc tggcaaaatg
    12841 ccactctgaa cctgagacat tgttcctcct gaaaataaga atgccacgca atccgtggca
    12901 ttgttgagcg ctattttacg tgttttttcg cggcggcggt ttgttcagac gcaaattaac
    12961 gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa ggcattaacc
    13021 cttcaggacc actccattcc ccgctgaact ggtagctaat atgttgactg ccttcggctt
    13081 tacattcgac ggcacgatta tcatcaccat cagctttttg gcaattgcca aatgctttgc
    13141 tgtaaaggtc gctaaacggt gtaccgattt taacaccggt gtcagcagga atatcgctat
    13201 ccagcacatc aatgcggctg atcgtaccct gatcgccgtt aatcaccatc gccacgttgt
    13261 cgcctttcat cacttcaaaa aagcgcacca cgttgccgtt cgcggttttc attccgctgc
    13321 gcaggcgata atcgccatca agcgcatcgg caatggcttg ttcttgcagt ggtgtggacg
    13381 ccgttaattc accaacgccc tgctcgctca ctttggtgga cgacccaaac cagttccacg
    13441 ggttagcggc agaccagtta actgacgaca tcgtggaaca gccggtcagc atcaacggca
    13501 tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt caataacgtt
    13561 gcttggagtg caaattcacc aaaaagtgcc gttattcttt ttcttcatta aaacaagcgc
    13621 gtaaacgtcg attggtcagc agccagatta acgccacaat atccgccact accagcgcca
    13681 gcccgatacc gctaacggat tcaccgttca gccacagcca cggttgccag caaagtaaaa
    13741 ccacctgcgc caacagcaac agaaaataga gcacatgcca gatacgggga aatgaagccc
    13801 gccgaccact cagcaaaaac gccagcaccg ccggaatgcc gggaatcaac cccagccaga
    13861 aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctcg cgcgacgcac
    13921 cagcaatgac aaacagcacc caggttcgcg cctgaagcaa tagcacaagc cagaagagca
    13981 agggtaaacg caggcgaccg tgcgcatcat aatggacagg atgaaactca gtactcttca
    14041 tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc caggcgttca
    14101 tacattccga gcaccatgtc gttatcttcc ggcacgttga tctgaatttt cggacagccg
    14161 cgagcaatca gctttttctc cagccgatta agcaacgcat tagcaatccc acgcccacga
    14221 aactctggat gcacgccaag ataataagca gacccgcgat gcccgtcata accgcccatc
    14281 accgttccga ccacttcacc gttcacctca gcgaccagaa acaaactgac gtcatggttc
    14341 atcttacgct cgatgtccat ttccggatcg ttccacggac gcagcaagtc gcaacgctcc
    14401 caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc catggtattc
    14461 gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt agccaatatc
    14521 tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg aaatgaaaag
    14581 taaaacaatt ctcatcagca aacggtcgta acggattacg cgatacgata taacatctgg
    14641 aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa acactcactt
    14701 cgcgccgcca ggtgctgaaa gccggtttgg ctgccctgac gttgtcagga atgtcgcaag
    14761 ccatcgccaa agacgaacct ttaaaaacca gcaacggaca cagcaagccg aaagccaaaa
    14821 aatctggcgg caaacgtgtc gttgttctcg atccaggcca cggtgggatc gataccggag
    14881 cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcgattgct aaaaacgtcc
    14941 gttccatttt gcgtaatcat gggattgatg cgcgtttaac gcgttctggc gatacgttta
    15001 tcccacttta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg tttatgtcaa
    15061 ttcatgccga cggctttacc aacccgaaag ctgccggtgc ttcggtattt gccctctcta
    15121 accgcggtgc aagtagcgca atggcgaaat acctgtctga acgcgaaaac cgcgccgatg
    15181 aagttgctgg taaaaaggcg actgacaagg atcacctatt gcagcaagtg ctgtttgatc
    15241 tggtgcaaac cgatactatt aaaaacagcc tgacgctcgg ctcgcatatt ctgaagaaga
    15301 ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt gtggtgttga
    15361 aatcgccgtc ggttccttcg gtgctggtgg aaacctcgtt tatcaccaac ccggaagaag
    15421 agcggctgtt aggcacggcg gcatttcgcc agaaaatcgc cactgcgatt gccgaaggcg
    15481 tgatcagtta tttccactgg tttgacaacc agaaagcaca ctcgagaaag cggtaagtga
    15541 tgaaacccga cgcacaccag gttaaacagt tcctgctcaa ccttcaggat acaatttgtc
    15601 agcagctgag cgccgtcgat ggcgcagagt ttgtcgaaga tagctggcag cgcgaagctg
    15661 gcggcggcgg acgtagccgg gtgttgcgta atggtggtgt tttcgaacag gcgggtgtca
    15721 acttttcaca tgtccacggt gaggcgatgc ctgcttccgc caccgctcat cgcccggaac
    15781 ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg cataatccgt
    15841 atgttcccac cagccacgcg aatgtgcgct tttttattgc cgaaaaaccg ggtgccgaac
    15901 ccgtctggtg gtttggcggc ggcttcgact taaccccttt ctatggcttt gaagacgatg
    15961 ctattcactg gcaccgcacc gcccgtgacc tgtgcctgcc atttggcgaa gacgtttatc
    16021 cgcgttacaa aaagtggtgc gacgaatact tctacctcaa acatcgtaac gaacaacgcg
    16081 gcattggcgg cctgttcttt gatgacctga acacgccaga cttcgaccac tgttttgcct
    16141 ttatgcaggc ggtaggcaaa ggctacacca acgcttattt accaattgta gagcgacgta
    16201 aaacgatggc ctacggcgag cgcgagcgca atttccagtt atatcgtcgc ggtcgttatg
    16261 tcgagttcaa tctggtctgg gatcgtggca cgctgtttgg tttgcaaact ggcgggcgca
    16321 ccgagtctat cctgatgtca atgccgccgc tggtacgctg ggagtacgat tatcatccag
    16381 aagatggcag cccggaagcg gcgttgagtg agtttattaa ggtcagggat tgggtgtaac
    16441 tccctcaccc ccattcccgc atccgctgat gcagcgtcag tgacggcttc tcggcaaaca
    16501 gctgctggta atccgtggcg aattgcccca gatgccagaa tccccactgc atggcggcgt
    16561 cttttaccgt catactttgc gaccacgggc ttatcagttc gcggcgcacg gcgttcaggc
    16621 gaatgcgttt cagccacgca tttgggccaa tgcctaaaat agcgtgaaac gcgttttgta
    16681 gcgtgcggcg gctgacatgc agttgattac acaagtccag caccgtcacc ggttcggaca
    16741 tgttttccag cacatattca cgggcgcggg aaagcaaccg acggtaactc tgatgactga
    16801 tgctttccgc cgtcaccatt ggctgcgctt cttccagcat cgcccccatc gccattagca
    16861 aattatcccc cagcacttta cgcacagcag gctgatgaag attttccggg ttctcgcaaa
    16921 atgtcgccag cgcctgttgg acaaagcccc acagcgcggc tttatgctgc tctttcactt
    16981 ccaacgccga ctggctacgc agcatatgta atacccgatc cggattatgc aaaaagttag
    17041 cctgacgggt gatgacatct tcagaaagca ctacgccaag gatcgtgtaa tcatccggcg
    17101 tacttaattc aaactcggtt ccaccagggc gcgtcgctat ttctgcgctt cccagacatt
    17161 gcgaaccgat aaatccctgc tctccgcgcg tcgccggaat gccaaaccag aacgaattcg
    17221 gccagaccag gcacgactga cgcagcgcca gaccggtgta ttcacgaaaa acctgaatat
    17281 catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc tgatcgtaaa
    17341 tctgctgcca ggcggtaatc gtcaaagcat gttcatagac atccgttgtc tgtcgttgat
    17401 gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag ggttcatgat
    17461 aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg ggtgttgtag
    17521 gacacccggc acctccgaca ggttaatggg gcttgagacg ataacgacta ctgcgtttac
    17581 gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc agcggccagc
    17641 caaagtgggc agccacttct cctgccgtca ttccctgacg tactgatgtt aacagcgcca
    17701 acaactcgtc agcagattcc acgacgataa ctggcgcgtc gggttccttt acgggttgct
    17761 tcggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc cgaccgatct
    17821 ccttacggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg tccagcgccg
    17881 cacgacacgc cgccagatcg ccttccacca ccagcgttag gcgaccaggg tcaagcactt
    17941 cgtgactgag cagacgaacg ttagctgctt tgagcatggc atccgctgca tctatcgcgg
    18001 cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac ctccgttacg
    18061 cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaagcg ttacaggctg
    18121 ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt tcagacggcg
    18181 gcgggacata ggtcaccagc tgcacgtcgg cagatttcaa cgccgcatca atgccgtagg
    18241 tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg atcccggcgg
    18301 ttgatgagag ataagaaccg gtacgcgaaa ctacatgtgc caggaaagct gtgttttctg
    18361 cgtcattagc ccactggaaa gccgcgccat tttcaatatg cgcaaccatc gcatccagac
    18421 cagcacgcac ttccgccggg tttggcccac cgagcataat cagcacttca ccggcagtcg
    18481 gtgacggtcc gtgtgcagct cccgcataca gcgagcggcc atacaccact tcgaccatcg
    18541 cctgcttggt cgcttcgtca gccgcaatgt aggtgacgtc atcggaatca gcagaaatca
    18601 gcccaaggct acgaatatgc ggcggcaatt tcagttcacg cgcaaattcg gcgttaacag
    18661 aggcaatcac ccgcatggcg gtgaccgacg gtcgaatcaa atctaaagct ggcatgatgc
    18721 ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt ttcgccaaat
    18781 ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg ttagagatac
    18841 aggtacggtc ggcctcaacg gtggttgcca tacgcggcga gtaaacggcg tagcaggaga
    18901 ggctttctga ctgccctaat cccgggcgtt cccccaccag taggatcact actttcgcgc
    18961 cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgaaca aagaatggcg
    19021 taccgacttt cagcccggcc tgtttcaggc ccgccatcag cggtggcagg atctcttcgt
    19081 agttcacggt gatcgcatca gttgacaggc catcagaaat aacgacctgc acgtccggat
    19141 tggcaacaca ctgcgctttc agcgcttcaa cagcttctgc acacaggcgg cggcccatat
    19201 ccggacgagt caggtagaga tttttgtcgc taatttcaga gcggacttcc agcaagccct
    19261 gcgctttcac ccactcttcc ggcacttctt tcagtacggt atctttcgaa cgagagtgat
    19321 cggccaggaa acgcagcaac gcctgggtac gcggacgcgg accggcacga ccggtacaaa
    19381 cgcgggccac ggtgctgcga cgcagttctg ttaatacgtc tgcgcgatgc gggttttcaa
    19441 cgccaatcca cgcttttgct tccgcagagc ctaaatccag cgcgcagctt tccgaggtca
    19501 ccggtgccgc acaggtggtg gtggcgcact ttgcttctga cggcgcgggg gccgcttgtc
    19561 ccattgacgc catcacgctg cgtacaattt cttcaatctg tttttgatcc atgatgtgtt
    19621 atctccgcgt catcagaaga acagtgacgg atcgcccgcc cgtttggtca ggcgaccgtt
    19681 tgccataatg cccatgcttt ccagccagcg ttcaaactcc ggtgacgggc gcaggttgag
    19741 taactgacgc acagtggcag tgtcgtggaa tgcggtggtc tgatagttga gcatgatgtc
    19801 atcgcccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca ggatcatcag
    19861 gttttcgttg aggttctggt cagcgtcagc gtggttggtg tagcagcagt cacagcccat
    19921 agagatgccg ctcagcttgc ccataaagtg atcttctaag cccgcacgga taatctggcg
    19981 gtcgttgtag agatactccg gcccaataaa gccgaccacg gtgttgacga taaacggatc
    20041 gtaatgacgc gccagcccgt agttacgcgc ttccatcgtc acctggtctg cgccgaagtt
    20101 agcgccagcg gatagcgcag agccttgtcc ggtttcgaag tagaggcagt tttccccggc
    20161 gatacggttg aactccgcgc ccactgcgcg cgcttcgtcg agcatcgcca gttccacgcc
    20221 aaactctttc agcccttttt cgctgccgca gatactctgg aaaatcagcc cgcctggtgc
    20281 gccacgacgg atcgcttcaa tctgggtggt gacgtgcgcc agcacgcagc cctgggtcgg
    20341 aatgttgaat ttgtcgatca cgccatagat ggtgtccaac acgcggctta agttttccac
    20401 gtcgtcagtc accgggttaa cgccgatcac cgcatcgccc accccgaaag aaagcccttc
    20461 gtagatttgc gcggcgatac tttgcacgtc gtcacgggtg tcgtttggct gcaagcgggc
    20521 gctgaaggtg cccggaatac cgatggtggt attggccttt ttgatcaccg gcattttctt
    20581 cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcga ccacttccga
    20641 ggtcagccct ttgcgggtaa aggcaatgtc gtccacgctg gtttcatcgc tcagcacata
    20701 ctcacgcagt tcgctgatgc tccagttttt aatctggttg taggccgttt cgttaacgtc
    20761 gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat tattgcggat
    20821 gtccgctacg gtcatttccg acaacacctg ctttgccgcc acgcgctcct gtgagcttgc
    20881 cgctgcaacg cccgccagca catcccccga acgcagttcg ttggctttag ccagcacctc
    20941 ttttacatcc ttaaactgat atacattgcc gaacaatgtg gtctttagtt tcataagtcg
    21001 ttccctcagg aaggaaatgc gagtgatttc accgtcaccg gcacaaccga tccgccaaaa
    21061 agaggcgtac caatgtcgat atagtccccc gcgcggacaa tcacttcgtc aatgactgcc
    21121 aacgggagtt gttgtagctg tgggcgcaac aacatgccca gagctttacc aaagtcctgc
    21181 ccggccacca ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc gaccagcgcg
    21241 ttgatgaccg tcagtaccgc ggcgtaacgc acaggcagcg aggcgggaag cgccagcacg
    21301 tacgcgtcag aattgggatc aagatccagc tgaatcagcg cctgttgcca ggcactcacc
    21361 agatccgttt catcaattgg gatcgccacc ggcaaattgc gcagcggcag ttgtacgccc
    21421 tccagccaga ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc aatcaccgtg
    21481 gcgcgtacgg tttgcgctgg aaactgcacg ttcatctcac gcaggcgcgg atggtcatgc
    21541 agcgccgttg ccagaagcgg gccgatatcg gcaaaacaga acggatcggc gggctggtgg
    21601 cgataacatt cgcccacgcc gccagaaagc gtaatgattt cgggcgtaac acctgcgggc
    21661 agcaaaccgg tttgcattaa tgcctgcgcg agcggcgaga gcgttccgtc aatcacttcg
    21721 acaatcagct ctgccatccg ccgggtaacc tgcaccagct gcgcgccggt cagcgaacgg
    21781 gcgtcggtgc ctgcgccgaa gcactcatcc acaatcatct gccccggttt atgagcgtaa
    21841 accacgcgcc cctggctgtc ggtttccagc aggcgaccac cgacgttgag gcaggcagtg
    21901 ccgctgattt ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc gatgtcgata
    21961 ttcagtaccc gacacagccg ttgttcagaa agggtttgcg ccccggcacc gtgaccggcg
    22021 atcacggatt cgagatgcgg cccggcgctg gcgacgacaa aatcgcccag cgactgggag
    22081 agcgtcatca ccgccgggcg agcgttgcgg gttttcgcgc tttcaccggt gatgatgatg
    22141 gcaccagaat caacactttc cggcgcaatg cccgcagcct gatattgctc caggattaag
    22201 gttttcagtt ccgcttcttt taaaccaccc tgtttatcga caggggtaaa gaacaccggg
    22261 ctttgccagc taatttcgcg tttgatgaat tcgtagcgcg gcacctgcga caccgccgca
    22321 cggttaacca gctccaggtg ggagaagatc acctgggtgg tggtggtgcc gatatcgata
    22381 ccgacgctca gtagctggcg agtgttcacg attgtgcctc cacttcggtt ttggccgcgg
    22441 tcgcgtcttc ttttggcacc agcatcatcg ccacgccaat cgccgttacg ccgccgatca
    22501 acttgccgac aatcatcggg aagatcatgg cgttcatgtt ggcagcggcg aagcctaagt
    22561 ggtcacccag ggcgaaagca gcggaaacgg cgaaggcaca gttgatcact ttgccgcggg
    22621 tatccatctg cttcatcatg ccgaacatcg ggatattgtt ggcaagggtt gcgaccatgc
    22681 cagctgccgc gatgttgttc atattcagca ccttaccgac gctcatcagc ggtttttcaa
    22741 accagcgagt cagcagcagc accatcggat acgcccccag cagcacgcag gagatagaac
    22801 cgataacttc aatggcgcgc atcacttcac cgggtttgtc gcctggagcc atgaagatcg
    22861 gatccagacc ggggatcagt tcccaaccga gcaggaattt cactaccgca gcggcaagac
    22921 cgagggtgat caatgcaacg aggaatttgg cgaagatctg gaagccgttg atcatttttt
    22981 ccgggatgaa tttcagcccc agcgccacca gtaccgcgac gataagtacc gggatcatgt
    23041 tcatcaggat cagggcaaag gtgaattcca ctggctgacc gttgatctgc acaccggaat
    23101 acatagccac cagaccaccg gcaatacagc caatcggaat ggtcacaatg cccgccagca
    23161 cgccgagcgc cagataacga cggtcagaag gttcgataat gccgagcgcc accggaatgg
    23221 aaaacacaat cgttggaccc atcatcgacc cgagaattaa tccagagtat agccacgctg
    23281 ctacgtcgcc gcccgccagc tctttggcga ggaagaagcc gcccatatcg cacgccagca
    23341 gtgttccggc gaacattgac gggtttgcgc cgagcatttc gtaaaccggg ataatcaccg
    23401 gcccgagtac gtgggccaga acaggtgcca gcgcggtcat accaaccatc gccaggccca
    23461 gtgcgcccat cgccataaag ccttcttcaa actgcccgcc ggatccttcg atacttttac
    23521 cgaacttacc gaggaaacga gcagaaccgc cgaactgcga caggatcctg tctacggcag
    23581 ctatcagcat aaagaacatc atgatgtaca tgatgatttc gttaattccc atcgccttta
    23641 ctccctgtta gttgttattt attggcggat gcggcgtaaa cgccttatcc gccctacatg
    23701 tgcaatcctg taggccggat aagacgcagc aagcgtcgca tccagcaatt gcaccgcgcc
    23761 actggcggat gcggcgtaaa cgccttatcc gccctacatg tgcaatcctg taggccggat
    23821 aagagcagca agcgttgcat ccggcaattg caccgcgcca ctggcggatg cggcgtgaac
    23881 gccttatccg ccctacatgt gcaatcctgt aggccggatc agacgcggca agcgtcgcat
    23941 ccggcaattg caccgcgcca ctggcggatg cggcgtgaac gccttatccg ccctacatgt
    24001 gcaatcctgt aggccggata agacgcggca agcgtcgcat ccggcaattg caccgcgcca
    24061 ctggcggatg cggcgtgaac gccttatccg ccctacatgt gcaatcctgt aggccggata
    24121 agacgcagca agcgtcgcat ccggcattta ttgtgccgct gcgtacaggc cgacaatctg
    24181 ctccaggctg gcggtacgcg ggttgctgcg cagacaaata tcttccagcg cggcctgcgc
    24241 ccatgcgccg taatgtccgg tggttgcgcc cacgtctgcc agccgcttgt tgatcccgac
    24301 ttccgcaatc agctcactta ccgcgttaat agcgtcacga tcgtcggatt ttttagttcg
    24361 cagcgcccga ccaatctgac taaagcgttc acgacagacc atccggttaa actccatcac
    24421 cgttggcagt agcatggcgt tcgccagacc gtgcggaata tgcagcgccg cccctggctg
    24481 atgcgccatc gcgtggcaca acccaagccc cgcactggaa aacgccattc ccgccataca
    24541 tgaagccagc aacatgctct cgcgggcggc aaggtcgtga ccgtagccca ccgctttcgg
    24601 cagcgatttg ccaatcatcg caatggcacc aatcgccaga ctgtcggtaa acggtgtggc
    24661 gttcagcgcg ctgtacgctt caatagcatg ggttaacgcg tcgataccgg tcatcgccgt
    24721 gacatgcgac ggcacgcctt cggtcagcgc agcgtcgaga atcgccacat ccggcatcag
    24781 cgaggcatgg gctaacacct gcttgcgccc gctcaccgcg tcgataatca ccgttacgtt
    24841 ggtggtttca gagccggttc cggcggtcgt tggaatggca atcaacggca ggcgcggttg
    24901 cagaacgctg gtttctgaca tctctgccag cgtgctatcc gggttcgtca ccagcaacgc
    24961 cacggctttt gctgcatcca gcaccgagcc gccgccaaac gcgatcaccc cgtcacaacc
    25021 tgattcacgc aactgtgcca cggctgcaca cacgtcggtg atgcacggtt cgcccaccgg
    25081 acatggccag agcgtcatgg cgataccttt aacggccagg ctgcgcgtca gcccggcggt
    25141 catccctgcc tgatgcaaaa agctgtctgc catcacgaac agatgtttca gcccacgcgt
    25201 ttgcgcttgc tgtccgcaac tgctcaccga gcccggaccg caaagcgtca ccggtggaac
    25261 gctaaatgtt tttacccgtt gcagattcag ggtatcgaac gcctgaaaga gcgcggtctg
    25321 caattcattt tgcatagatc ccctccgctt tctctcgccc gctactggcg atcgccagcg
    25381 gggtcataaa caaactgtgc tgcggtaaat gcacctgtaa cgccgggaat tgtttgcgaa
    25441 acagttccgc cacgcccggt tgcatacaag agccgcccgc cagccataaa tcagtaatgc
    25501 cctgcccttc aatatgccgg gcaacaatgt ccgccatttt ttcgtacacc ggtttcaccg
    25561 cgggccaaat ctcttcaccg tgaccgcgct tgtactgctc tgcctcttcc agcgaaatac
    25621 gacgatttcc ggcgagagtc agagagatgt gatgtccgcc ggtcgcttca tccgccgagt
    25681 acgtcacctt gccctttttc acgatggcaa taccagtagt gccaccgccg atatccacta
    25741 caccggcgtt gtccagttgc agcagatccg cgactgcggt tggctcatcc agtacatggc
    25801 tcacttccag tccggcagat tccagcacgt tgatggaaat acgcgggtcc gtacctggag
    25861 gaaaagaggt cgccgcatgg ctaaaacggc gaccgaattg ctgctcgagg gtgtcgagat
    25921 ggcgacgaac aatggtgacc gcaccgaaga aatcccagac gatgccgtcg cggaccacat
    25981 cagcccagtc gaggcatacc gccaccggct gaccgtcgcg gtcgacaacc atcgacacca
    26041 catcgcaggt gcccagatcc acccccagcc acagcggaga ttccgttgcg gcaggcgtct
    26101 ggttacacag cgttgccgcc gtttgcagac gcggggtgag ccattgttcg tcgtgcgcca
    26161 tcggttactc cttaaacaat gcgaaacgca tcaactaata cacagcgacg cagacggaca
    26221 aacgtacgcg cgctggttac cccttcaccg gttggcgtgg tgatggtcat ggtggtccag
    26281 ccttccccgc ccagccccag cccggcaatg cacggtccgt tcttaacgaa aatgctggta
    26341 tcaatggcgt tcgccatctg gttcatgttc tcgatattgc gcgagtgcat tgccgccgtg
    26401 tggtggcaac cgccttccag tttcaccgcc agcgcaatgg catccgccac gttggcgacg
    26461 cgcacgacgg gcaacaccgg catcatcagt tcagtcacgg caaacggatg ttctgcggtg
    26521 gtttccacaa acagcaggcg cgtttcttgc ggaactttaa ggccgattgc cgccgcgatt
    26581 ttgcctgcgt cgcgaccaac ccagtcacgg ctgacggagc cttttccgcg ctcgtcgata
    26641 tttttcagca acaccggttg cagctgctgc gcctgctctg cggtcagttt caccgcgtgc
    26701 tggccttcca tcagacgcat cagttcatcg gctacgctat ccacaacaat cagtaccttt
    26761 tcgtcggcac aaatgatgtt gttatcgaaa gacgcgcctt tgacgatgga ctgagcagca
    26821 cgggcgaggt cagcggtttc atccaccact accggcgggt tgccagcgcc tgcggcaatc
    26881 agacgtttat tggtgtgttt acgcgccgct tctactaccg cttcgccgcc ggtcaccacc
    26941 agcaggccga tgcccggaaa cttgaacaaa cgttgcgcgg tttcgatatc cggatttgcc
    27001 acagtaacca gtaagttttc cggcccgcct gcggcaacaa tcgcctggtt gagcagcgta
    27061 atcgcccgct gggagacttt tttcgccgcc ggatgcgggg caaaaatgac gctgttgccc
    27121 gcggcaatca ggctgatggc gttattaatt acggttgccg ccgggttggt ggacggagtt
    27181 accgaagcca ccacgcccca gggcgcgttt tcaattaaag tcaggccgtt gtcgccagtc
    27241 agcacctgcg gagagaggca ctcaacgccc ggtgtgccgc gcgcctgagc gacgtttttg
    27301 gcaaatttat cttcgacgcg gcccatgccg gtttcactga cggcaagttc cgctaaatct
    27361 ctggcgtgtt tttcgcctgc ttcacgaatg gcagcaatgg ctaactggcg cattgccacg
    27421 ctctttaacc cttgctgggc aattttggct gccgcaacgg cgtcatccag ggacgcgaaa
    27481 acgcccatct catgaacggc ggcgggcggc gtatcactgc tttgcatttt cagcagtacc
    27541 gctttcacca cctgttcaat atcctgttga ttcatgatgt tctgccttat ttgtggaaaa
    27601 ttacctgacc gccagacacc acctcatcga tgatgccaat cacgcacaga tcgaccggtg
    27661 acgtttcgct tttatgcgcc tggcgggcgg aactgccact caccagcaac acccactccc
    27721 cggttcccgc gccaatattg tcgatggcga cggcgcattg cccgtcagga ttaccttgtg
    27781 gatcaatcat ttccaccatc agcaatttgt catgcgccag tccgtgatgg cgtacggtac
    27841 aaacaatttg tccagtgacg actgccagtt tcatacccgc ctccgtggcg tatttcaggt
    27901 aaaagctccc cctaccctcc gcggaaggta aactgaaaaa ggagagagcg tgacgcccga
    27961 atcgacgtca cacagggtga ttacaggttg ctgctatcgc ctttcaggcc gatcgggaag
    28021 acttcttcca ggtcgccgtg cggacgcggg ataacgtgta cagataccag ctcgccgata
    28081 cgctgtgccg ctgcagcacc tgcgtcggtc gcggctttac aggctgcaac atcaccacga
    28141 accatggcag tacacaggcc gccgccaatc tgcttaacac caaccagctt gacgcgcgca
    28201 gctttcacca tcgcgtcaga agcctcaatc agtgcaacca ggccccgggt ttcgatcatt
    28261 cctaatgctt ccattgtgct ttcctcttta tcagggtcca gaacgggacc gttcattcaa
    28321 ccagtgtttg taaactgctt tcgcggttca cttctgtctg acgcggcaca gctgccacca
    28381 gcgccagctc gataatttcc tgcacgctac aaccacgaga gagatcgtgc atcggcgcgg
    28441 caagtccttg tatcaatggc ccgacggcac gatatccgcc gagtcgttgt gcgattttgt
    28501 agccaatatt tccggcttcc agcgacggaa aaaccatcac attggccttg ccttgtagcg
    28561 ggctggcagg cgctttttgc gccgccactt ccggcacgaa ggcggcgtca aactgcaact
    28621 cgccatcgac taccaactgc ggcgcgcgct cacggacgat ttctgtcgcc tgctggacgt
    28681 tggcaacgca ggggtgacgg gcgctgccgt tactggaaaa cgacaacatc gccacgcgcg
    28741 gttcttctcc ggtgatggcc tgccaggttt cggcgctggc aagcgcgata tccgccaact
    28801 gcgccgccgt cggctgtggc accacgctgc aatcggcaaa acccaacgcc ggaccgctgt
    28861 actgtggcag catcaggaaa atggatgaga gcgttttaca gcctggctgc aagccgataa
    28921 tgcgtaatcc ggcacgcagc acattcgccg tggaagagag gttgcccgcg atacagacat
    28981 ccgctttacc ggcgctgacc attgctgcgg cgaacatcag cgggtcggta agtttttcca
    29041 gcgcatccgg cggcgttttt tcgcccgcgc gggccagcca gcgatgagca aattcttccc
    29101 gcattgcgag gttgccatgc ggatcaatca cctgtagccc gtccatcgct acgccatgac
    29161 tgagcgcaaa ctgacgaagt tcaaacggat tagcgaccag aatgggcgtt gccagacctt
    29221 gttgatgtaa atattgcgcc gctttcagca cacgctgatc taacgcatcc ggaaaaacca
    29281 ctctggcggg cgctcgcaac gccagttcac gacaacgttc aataatcatt gcgtttcccc
    29341 cagtcgttgt tggatctgct tcaccgttag cggcggcttc gttacgctca ggatcatcat
    29401 cacgtagacc gtgctggaaa ggcggttcag cgcctgcaag atgtccgggc gtaatacttc
    29461 aaagctgcgg gtgataaaga cctgcgccgc tacggtttcg gtttcccgca ctttggtgcg
    29521 tagcagattg agcaacgccg catcgcgccc gtggctggct tccggcacca gatgatcgtg
    29581 gtcgagatag cgcagcggct gatgagaaag ccgatgcaga tcttcgtcag aaagcccgac
    29641 aatcgcttga tcgcccagcg gttcccccag cgcatcggca cgcataatgt tgccaagacg
    29701 cgaacggata tccgccagcc acggctgcca cggttccgcc agttcaattt gcagccacac
    29761 tgccagcgca atggtgctgt cgagaaccgc gcgaaaaccc aggcgcggat cgcttttggc
    29821 gaccattttt tccgccgaca ggtgagtcag cgtatccggc tttttcgcca ccggctggcg
    29881 gcacagttcg cagcacgcct gcggatgttc atcgctactg gttagcccat gaacgggctg
    29941 cggctgctgc tgttcatcgt caacaaacag gcggccctgc tcgtcaataa acttgatgcg
    30001 cagatggcgg ctttccagca actcccgggc agagggcgtc aggcgactgt ccgcagggag
    30061 atggatctct gcgccttcgc tgagcgtatg gttcgctctt agccatgctt cggtgatgaa
    30121 atctttcata gggattgcca gttagccggc caggcgacat acaggaattt cacgctggat
    30181 gtcgtaccaa attcgatgct ggagcctttc gggataaaca tcacgtcgcc tgctttggcg
    30241 atcatggttt cgccttcgtg gcgaacgtgc agttcccctt ccagtaccat gtcgatttcg
    30301 tcatagttca gggtccacgg gaagaaggcg ttctcccact gcataaaacc agcggccatg
    30361 ctgctgccgt cgtctccggt gaccagatcc gttaatccga cgcagtgtgg ctgtgcgccg
    30421 tcgaaacgac caaacttgac gctgctgcca tcaataactt tgataccgcc tttgccggtc
    30481 accgatttga agctcggctg cattgcgccc tgctccagcg actgtttttc cttcatcact
    30541 ttttccatca gctgcgcgac caggctttcg gtaaactggc cttccggcag ctgggcaatg
    30601 atggtttcgc ggatgcgctg gctttcggtt ttatcggctg gcacgctggc gggaacggac
    30661 gccgttaccg gggtcgattc atcacattca gtgatggtaa aacccagtag atctgccact
    30721 tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc ctgttcgccg
    30781 cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg
    30841 tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt
    30901 tagctcgaaa atcggctctt cgaagcccgt ctcaagcaat aattttcgcg ttgcggcgac
    30961 gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc tgacgccaat
    31021 atccagcaac ccggcaggca accgactttc cgggtcattc gcgccgtgga catagatcag
    31081 catgtccaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat ggctaaaata
    31141 ttcacccggc gtatcaatat cacccttatc attaaattct acggcctgcg tttttctggc
    31201 gagggtataa tctccctgca acgcattaaa taacgttgtt tttcctgccc cgacggtacc
    31261 cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt cacaaagcgt
    31321 ataatttaat aaacgcccta aaccgctgac ggtttgtgat aacgcttctt ctactgcacc
    31381 caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga tatgcacatc
    31441 agcggctttc aatgctaaat caccggcgat catcgccgtt tcgccaggag ttagcgtcat
    31501 aatgccgatt gcgccagcat ccggaacgcc gattttcttc gccagttcct cgccgggatg
    31561 cgcgatgaga tgcgccagcg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc
    31621 tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga
    31681 gtaaataaca aaattcggca agtacgttta aaaggtgagg tagatcacta agaatggaga
    31741 gataattact gttatgaata aactttcata tgcgacgcat gaatataaat aaaataatct
    31801 cgagatattt tatatactgc tttttttatt attcgcaaac tcagtaataa gctttactaa
    31861 tatattgcag ctaaaccgct taccctgaat attcagggta agcgtgagag ttaaaaaaga
    31921 attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatgttg acgatacgac
    31981 gtaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc agcaccggac
    32041 cgacagtcac accttccgag ctggaaacac gcagtaagtt gtaactaatg cgggcagctt
    32101 ccatgttcgg catcaccaga atattagcgg aacctttcaa agggctgtcc ggcatacggt
    32161 cgctgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagtt
    32221 ctggtgcacg ttccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt
    32281 cagaagaacc aaagttggag tgagacaaca gagccacacg cggctcaata ccaaaacgac
    32341 gaacagtttc tgccgccatc aaggtgatct ccgcaagctc ttctgcgtct ggttcgtcat
    32401 tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgcg ttcatggcac
    32461 ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacgcta aaatgttcat
    32521 gataatcgcc caccgtaccg cagatcattg catcggcttc gccacgctga accatgatcg
    32581 cgccaatcac tgtcgggtta ctgatcaatg cacgctgcgc ctgttcctga gtgacaccgc
    32641 gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt
    32701 tattgacgat ctcaaaatca acacccgctt tgatctgcaa gcccagtttc tgaatgcgca
    32761 tttcgatcac gttcggacga ccgataagga tcggtttcgc cagccccagc gttaccagtt
    32821 cctgagtggc atgcagaacg cgcgcctctt caccttccgg cagaacgacg cgcttcggcg
    32881 ctttgcgagc ctgggaaaaa atcggcttca taaacaggtt ggttttgtaa acgaactccg
    32941 tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg ccggactcca
    33001 tcgctgcttt agcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg
    33061 gaatgatgta ttccggacca aagctcagat cctgatcgcc ataagctgaa gccaccactt
    33121 cactctgttc cgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt
    33181 cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg aagcaaagaa
    33241 cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca tccggacgca
    33301 cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg
    33361 gcgcacgggc cattttcttc accatttcct gggtcagcac tttcgggccg gaacagccga
    33421 ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca tccaccaccg
    33481 catatgcggc tttggtttcc gccatgtttg gctcacggcc ctgatagata acgccttttg
    33541 agtcgcaaac cacgatgtta tgtttttgca gacccagcgc taccagcagg ttcatacagg
    33601 cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca
    33661 ccacgcgcaa accgttgagg atggcggcag tgctgatgat tgccgtgccg tgctgatcgt
    33721 cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg
    33781 gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcaagcgcg gcgacgactt
    33841 caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca atcccggcga
    33901 atttcttaaa cagaacgccc ttgccttcca tcaccggttt gcctgccagc gcgccaatgt
    33961 tgcctaaccc cagcactgcc gtaccgttag agatcaccgc caccaggtta ccacgcgcgg
    34021 tatatttgta ggcttttaac gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg
    34081 gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga gaaacctgga
    34141 ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac tggtcatcca
    34201 tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca gcccctgccc
    34261 acaacgtggc gcacattatt accttgccgg agtctacaga ctttgagcaa gtccaaactc
    34321 tcaccattaa tataatgttt tggtaataat cctataacac tgatgttacc tgcttaatcc
    34381 agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt
    34441 gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta tgaacgagtt
    34501 agacggcatc aaacagttca ccactgtcgt ggcagacagc ggcgatattg agtccattcg
    34561 ccattatcat ccccaggatg ccaccaccaa tccttcgctg ttactcaagg ctgccggatt
    34621 atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtagaaaaa atggcaaaac
    34681 ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat
    34741 tctcaaaatc gtacccggtc gcgtgtcaac agaagttgat gcacgcctct cttttgataa
    34801 agaaaagagt attgagaaag cgcgccatct ggtggacttg tatcagcaac aaggcgttga
    34861 gaaatcacgt attttgatca agctggcttc aacctgggaa ggaattcgcg cggcagaaga
    34921 gctggaaaaa gaaggtatta actgcaacct gacactgctg ttttcttttg cacaggcgcg
    34981 ggcctgtgca gaagcaggcg tatttctaat ttcgccgttt gtcgggcgta tttatgactg
    35041 gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccag gcgttaaatc
    35101 agtacgcaat atctacgact actataagca acaccactat gaaaccattg tgatgggcgc
    35161 gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc
    35221 accgaatttg ctgaaggagc tgcaggaaaa agtttcgcca gtggtacgta aattaatccc
    35281 accttctcag acgttcccac gccccgctcc catgagcgaa gcggagttcc gttgggagca
    35341 caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt tcgccgttga
    35401 tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg gagtgttata
    35461 tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgcg gtacaaaaag
    35521 ccaattctgg tcatcccggc gcgccgatgg gcatggctga tattgctgaa gtgctgtgga
    35581 acgattttct taaacataac cccaccgacc caacctggta tgatcgcgac cgctttattc
    35641 tttccaacgg tcacgcgtcg atgctgctct acagtttgct gcatctgacc ggttacgacc
    35701 tgccgctgga agagctgaag aacttccgtc agttgcattc aaaaacccca ggtcacccgg
    35761 agattggtta tacgccaggt gttgaaacca ccaccggtcc acttggacaa ggtttggcga
    35821 acgccgtcgg actggcgata gcagaacgta cgctggcggc gcagtttaac cagccggatc
    35881 atgaaattgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg atggaaggta
    35941 tttcccacga agtctgttcg ctggcgggca cgctgggact gggcaagctg attggttttt
    36001 acgatcacaa cggtatttcc atcgacggtg aaaccgaagg ctggtttacc gacgatacgg
    36061 caaaacgttt tgaagcctat cactggcatg tgatccacga gatcgacggt cacgatccgc
    36121 aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg tcgctgatta
    36181 tctgccgtac ggtgattggc tttggatcgc cgaataaagc aggtaaggaa gaggcgcacg
    36241 gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc tggcaccatc
    36301 cgccatttga aatccctaaa gagatttatc acgcctggga tgcccgcgaa aaaggcgaaa
    36361 aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggtccat ccgcaactgg
    36421 cagaagagtt tacccgtcgg atgagcggcg gtttaccgaa ggactgggag aaaacgactc
    36481 agaaatatat caatgagttg caggcgaatc cggcgaaaat cgccacccgt aaggcttcgc
    36541 aaaatacgct taacgcttat ggccccttgc taccggagct gctcggcggt tcggcagatc
    36601 tggctcccag caacctcacc atctggaaag gttctgtgtc gctgaaggaa gatccggcgg
    36661 gcaactacat tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc aacggcattg
    36721 cgcaccacgg cggctttgtg ccgtataccg cgacgttcct gatgtttgtt gaatacgccc
    36781 gtaacgccgc gcggatggcg gcactgatga aagcgcggca gattatggtt tatacccacg
    36841 actcaattgg tctgggcgaa gatggtccta cgcaccaggc tgttgagcaa ctggccagtc
    36901 tgcgcttaac gccaaatttc agcacctggc gaccgtgcga tcaggtggaa gcggcggtgg
    36961 gctggaagct ggcggttgag cgccacaacg gaccgacggc actgatcctc tccaggcaga
    37021 atctggccca ggtggaacgt acgccggatc aggttaaaga gattgctcgt ggtggttatg
    37081 tgttgaaaga cagcggcggc aagccggata ttattctgat tgccaccggt tcagagatgg
    37141 aaatcaccct gcaagcggca gagaaattag caggagaagg tcgcaatgta cgcgtagttt
    37201 cgctgccctc gaccgatatt ttcgacgccc aggatgagga atatcgggag tcggtgttgc
    37261 cttctaacgt tgcggctcgc gtggcggtgg aagcaggcat cgccgattac tggtacaaat
    37321 atgttggtct gaaaggggca attgtcggga tgaccggcta tggggaatct gctccggcag
    37381 ataagctgtt cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg
    37441 tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc tatatgttcc cctcacccta
    37501 accctctccc cagaggggcg aggggacggt ccgtgcgctt tttaggggcg catctgggat
    37561 tttgtttaac gggtgatcca caacgtgggc caggcgtctg gcccatgcca gttatcgcag
    37621 gtgggttctg cggcgtaacg caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc
    37681 tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc acgcgatttt
    37741 tcatcgaatg ttgcgttcat cagttccagt tcattcgtct cctgaccgct gttgaacggc
    37801 aaacgtaacc gaaccgggcg cgaggccagt ggctttttac gcgacacaat ccaggccaaa
    37861 tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagcgcttt atcatcggtc
    37921 agcgcagtga catttacctc tcgacgcaat ggatcaagcg agcagcgcag accattcatc
    37981 cgccagtttc cataatccag caaatcgttg cgttcttcga gtgagagtgg cgtcggcgtt
    38041 gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag cggcggttcg
    38101 tcccctttct ttatccacgc ggtttcactg ccaacgcgct tttgctgagc atcaataaac
    38161 aacaacgccg cttttaagcc attcagagaa atggtctgat tgccatcgcg taaggtgatt
    38221 gccttccctt cctgaatcat ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact
    38281 aataaccatg gtgaaatccg ccacttgtca ccacttaacg ccaacggctc gccatctaac
    38341 agcagccgtg gcgctatctc cccttcagac gcatccggcg acttcaaccc gccgcgctca
    38401 atacgtaaaa cggcatccgt atgcgccccg gcgctgcggc tcagggtcat caccagtccg
    38461 ttatgatcgc ccgtattacg cgccacgcag aagttttggt tattacaggt gacctgccag
    38521 tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtctgcaa caaagcgaaa
    38581 aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga ccccagaagt tcaaacaaac
    38641 agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca atcggattta tcggattgag
    38701 ccaataataa acaacgaaat attattgtta tttttcagtc cattaaatgt gacatttgca
    38761 aatagttaag caataacacc gtcttaccgt cacgtatctc gccagttttg atcatctcca
    38821 gcgccttgtt gaacggcagc tcgagcactt caatatcttc atcttcgaca ccaccgccgg
    38881 cgttagcgcg ctggctgtca ctgtattcgg cgataaaaaa gtggattagc tcagtcacac
    38941 cgccaggcga catatacagt tcaaataatt tgcgcacttc gcccacttca tagcccgtct
    39001 cttcaatcgc ttctttgcga atgcacactt ccggttcgtc gttatccagc agcccggcgc
    39061 aggtttcaat cagctgcccg ctttcattgc cattaaccca ggtagcgacg cggaactgac
    39121 gaatcagaac tacagtcttt ttcttcgcgt tgtacaggag gatcgttgca ccattaccgc
    39181 gatcgtaaac ttcacgttta tgacggataa cttcgccatc tttgcgcgtg agatcgtaag
    39241 taatgttgtg cagggtgaaa tagttatcgg agagaatttt gtctttaatg agggtgattt
    39301 gttgcgtcat accgactcca cagcgcgaaa tgaacaatta tcttacgctg tgaagccggg
    39361 tttgtctgcg caggctatca gtgagatttg actgatttta cccccagcca gtcaataatc
    39421 ccctgtgccg catgacgtcc ttctgccatt gcggtaacca ccagatccgc accacgcacg
    39481 gcgtcaccac cggcgaagat tttcggattc gtggtctgat aacggtactg gctttccaca
    39541 tccgcgatga tgcggcccca tttgtctacc gttacaccgt gcgactccag ccacggcatc
    39601 ccgtgcggat tgaagccaaa cgccataatc accgcgtcgg ctggcatgac aaattcactg
    39661 ccttccaccg gtactggacg ccgacgcccc tgggcatccg gctcgccaag acgcgtacgc
    39721 aggaaacgaa tcccgcaaac gtgaccttgt tcattcagct caagcgccac cggctggacg
    39781 ttaaattcga agttggcccc ctcttcgcgg gcgttcttca cctctttctt cgagcctggc
    39841 atgttagctt catcacgacg ataagcgcag gtaacgttac tcgcgccgtg gcgcagcgca
    39901 gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt gagtccggcg
    39961 gtattgataa acggctcttc cggtagctct tcgagtccca tcacctgttt agtgttggcg
    40021 atgaggaacg gcagggcgtc ataaacgccc ggcgcatctt cattgggtaa acccgctttc
    40081 atggaacggt aagtgcctac gccaacgaag accgcgtcgt attgttccaa aagcgaatcc
    40141 aaagagacat ctttacccac ttcacaattg agttcgaagt gaatccccat cgcgctgaag
    40201 atttcccgac ggcgtgccag cagggattta tccagtttga aagaaggaat gccgaaagtg
    40261 agcaagccac caatttctgg atggcgatcg tacaccgtca ccgccacgcc attgcgggtc
    40321 agaacatccg cacaggccag ccctgccgga cctgcaccga taatcgccac ccgcttgtcc
    40381 actttggtga catggcttaa gtcaggacgc caacctttcg ccaacgcctg atctgaaatg
    40441 tagcgttcaa tgttgccgat agttactgca ccgtgctcat cgcgaatagt acaggctcct
    40501 tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt gttggtctgg
    40561 tgagaaagct cgacggcggc gtcgatgttt ccggctttca ccagttcaat ccactgcgga
    40621 atatggttat gcagcgggca ggtccattca caaacgctgt gctcgccgca cttaaggcag
    40681 cgcgaggctt cccgttgtgc ctgatcggca cggaatggca gataaatttc atcaaaaccg
    40741 gttttacgcg cttcaatcgc cagtttgtcc ggctcgccac gcgcgggcgt tgcctgcatt
    40801 tgctcgactt tactcattac cggcatttct tgcgccgcgg tactggcatg ccacggttga
    40861 tgttcctgac gtgcggtacg caagcggcgg gatttcgcca tgcctgagag tgcggcgtca
    40921 gtgaccagtt gcagtgcgtc cgctgggcaa ttctcgacac aagcaggacc gttttcgcgc
    40981 cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc gacgggggta
    41041 aggacgattt gcatcgtacc aaatggacag gccaccacgc aggatttaca gccaatacac
    41101 ttttgctgat tgacctgaat gctgtcatca acgtggctga ttgcgccatt agggcagcta
    41161 cgggcgcagg gcgcatcttc gcaatggtga caggtcactg cactacgttg ctgctgatgt
    41221 ttgataaccg taattcgggg atgaaaatgg tgttggctca ggacatgttg ctcatcattg
    41281 tgagccatga cacaggcgat ttcacaagca tgacaaccca gacattgctg actgttggcc
    41341 ataataaaac gattcataac gaccttcttt tttggttgta aaaaccttat tctttatatg
    41401 agtgttgtta ttacccgact tacaggggaa tcggcaatgt tcatgtgccc agaataagta
    41461 actatttcgc tataaactgt ggcagatcaa ataatcccat cactgactaa attgcgtttc
    41521 agcgaactgg aacattaatg attttttgtg gagaagacgc gtgtgattgt taaacgaccc
    41581 gtctcggcca gtctggcccg ggcctttttt tacattgtcc tgctgtcgat tctttccacg
    41641 ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat caatattgcc
    41701 ggatcgctgc gtatgcagag ttaccgcctg ggctacgatt tgcaaagtgg cagtccacaa
    41761 ctcaatgcac atcgccagtt atttcagcag gcactgcatt caccggtatt aaccaatctc
    41821 aacgtctggt atgtgccaga ggcagtaaaa acccgctatg cgcatctgaa tgccaactgg
    41881 ctggagatga ataatcggct cagcaagggc gacttgccgt ggtatcaggc caatattaat
    41941 aattatgtta atcagataga cctgttcgta ctggctttac agcactacgc tgaacgcaaa
    42001 atgctgctgg tggtggcgat ttccctggct ggcggcatcg gtattttcac gctggtcttt
    42061 tttactttgc gccgcatacg ccatcaggtg gttgccccgc tgaatcagct ggttaccgcc
    42121 agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc tggataccag cctgccgaat
    42181 gagcttggcc tgcttgccaa aacctttaac cagatgtcga gcgagctgca taaattgtac
    42241 cgttcgctgg aggcgtcagt agaagaaaag acccgcgacc tccacgaggc caagcgtcgc
    42301 ctggaggtgt tgtatcagtg ttcgcaggcg ctgaacacca gccagattga tgtgcattgt
    42361 ttccgccata ttttgcagat tgttcgcgac aacgaagcgg ctgaatatct ggagttaaat
    42421 gtcggtgaca actggcggat tagcgaaggg cagccaaacc cggaattgcc gatgcagatt
    42481 ttaccggtga caatgcaaga gacggtttac ggcgaactgc actggcaaaa tagtcacgtt
    42541 tcatcatcag aaccgctgct taacagcgtt tcgtcgatgc tgggacgcgg tttgtacttt
    42601 aatcaggcgc agaagcattt tcagcagtta ttgttgatgg aagaacgtgc gaccatcgcc
    42661 cgcgaattgc acgactcgct ggctcaggta ctttcttact tacgtatcca gttgacgtta
    42721 ctgaagcgtt cgataccgga agataacgcc accgcacaaa gtatcatggc cgatttttcc
    42781 caggcgttga atgatgctta tcggcagtta cgcgagctgt taactacttt ccgcctgacg
    42841 ctgcagcagg cggatctccc ctctgcgcta cgtgaaatgc tggatacgtt acaaaatcaa
    42901 accagcgcca aactgaccct cgactgccgt ctgccaaccc tggcgctgga tgcgcaaatg
    42961 caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac
    43021 gccagcgaaa tcgccgttag ctgcgtcacc gcgccggatg gcaatcacac agtctatatt
    43081 cgcgacaacg ggattggcat cggtgaaccg aaagaacccg aaggtcatta tggcctgaat
    43141 atcatgcgcg agcgcgcgga acggctgggt gggacgctga ctttttcaca accttccggc
    43201 ggcggcacgt tagtgagtat tagctttcgc tccgcggagg gtgaggaaag tcagctgatg
    43261 taatgcctcc gactgaccaa agaatactgg cccttaaagt tcagtataaa agggcatgat
    43321 aatttacatt aactcctttt tttctccacg attggctcgt accttgccgc tacagtgaag
    43381 caagtcaagc ctacaacgat acgcagaaac acgaggtcct cttttaatgg cgaatttctt
    43441 tattgatcgc cccatttttg cctgggtgct ggcaatcctg ttgtgtctga cagggaccct
    43501 ggcgattttc tcattgcccg ttgaacaata ccccgatctc gcgccaccga atgtgcgagt
    43561 gaccgctaac tatcccggcg catcggccca gacgctggaa aacaccgtga cccaggttat
    43621 cgagcaaaat atgaccggcc tcgataatct catgtatatg tcgtctcaga gcagtggcac
    43681 cggccaggca tctgtcacgt taagttttaa agcaggcacc gatccggacg aagccgtgca
    43741 gcaagtacaa aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca
    43801 gggcgtgacg gtgcgtaaaa ccggcgatac caatattctg accattgcct tcgtctctac
    43861 cgatggttcg atggataaac aggatattgc cgattatgtt gccagtaata ttcaggaccc
    43921 gttaagccgc gtgaatggcg tcggggatat cgatgcctat ggttcgcaat attccatgcg
    43981 tatctggctg gacccggcga aactcaacag tttccagatg acggctaaag atgtcactga
    44041 tgccattgag tcacagaacg cgcagattgc ggttgggcaa cttggtggta cgccttccgt
    44101 cgataagcag gcgctcaacg ccaccattaa cgcccagtca ctgctgcaaa caccagaaca
    44161 gttccgcgat atcaccttgc gggtgaatca ggacggctca gaggtaaggc tgggcgatgt
    44221 cgccaccgtc gaaatggggg cggagaaata cgattatctt agccgcttca atggtaaacc
    44281 agcctccggg ttgggggtaa aactggcctc cggcgcgaac gaaatggcga cagcggagct
    44341 ggtgctcaat cgtcttgacg agctggcgca gtatttcccg catggactgg aatacaaggt
    44401 ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa gacgtggtga aaacgctgct
    44461 ggaagctatc gctctggttt tcctcgttat gtatctgttc ctgcaaaact tccgcgccac
    44521 gctgataccc actatcgccg tgccggtggt gttgatggga accttctccg tactttatgc
    44581 cttcggttac agcgtcaaca ccttaaccat gttcgcgatg gtgctggcga ttggtctgct
    44641 ggtggatgac gccatcgtgg tggtggaaaa cgtcgaacgt attatgagtg aggaaggact
    44701 cactcctcgc gaagccacac gtaaatcgat ggggcagatc cagggggcac tggtcgggat
    44761 tgcgatggtg ctttcggcgg tatttgtacc aatggccttc ttcggcggca ccaccggtgc
    44821 catctatcgc cagttctcta ttaccattgt tgcggcgatg gtgctgtcag tattggtagc
    44881 gatgatcctc actccggctc tgtgtgccac gctacttaag ccactgaaaa agggtgagca
    44941 tcacgggcaa aaaggctttt ttgcctggtt taaccagatg tttaaccgca acgccgaacg
    45001 ctacgaaaaa ggggtggcga aaattctcca ccgtagcctg cgctggattg tgatttatgt
    45061 cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt tcttgccact
    45121 ggaagaccgg ggcatgttta ctacctcggt acagttgccc agcggttcaa cccaacaaca
    45181 gaccctgaaa gtcgttgagc aaatcgagaa atattacttc acccatgaaa aagacaacat
    45241 catgtcggtg tttgccactg ttggttctgg ccctgggggt aacgggcaaa acgtggcgag
    45301 aatgtttatc cgcctgaaag actggagcga acgcgacagt aagaccggca cctcgtttgc
    45361 cattatcgag cgtgcaacga aggcgtttaa caagattaaa gaagctcgcg ttatcgccag
    45421 cagcccgcca gcaattagcg gtcttggtag ttctgcgggt tttgatatgg agttgcagga
    45481 ccacgctgga gcaggtcacg atgcgctgat ggcagcacgt aaccagttgc tggcgctggc
    45541 ggcggaaaac ccggagctaa cccgtgtgcg ccataacggc ctcgacgaca gtccgcagtt
    45601 gcagattgat atcgaccagc gtaaagctca ggcgctgggc gttgctatcg acgatattaa
    45661 cgacacactg caaaccgcct ggggttcgag ctatgtgaat gactttatgg atcgcggtcg
    45721 cgtgaagaaa gtctatgtgc aggcagctgc gccgtatcgc atgctgccag atgatatcaa
    45781 tctctggtat gtccgaaata aagatggcgg catggtgccc ttctctgctt tcgcgacctc
    45841 acgctgggag acaggctcgc cgcgtctgga acgctataac ggttattctg cggttgagat
    45901 tgttggggaa gccgcaccgg gggtcagtac cggtacggcg atggatatta tggaatcgtt
    45961 agtgaagcag ttgccaaacg gctttggtct ggagtggacg gcgatgtcgt atcaggagcg
    46021 gctttccggc gcgcaggctc cggcgctgta cgccatttcc ttgctggtgg tattcctgtg
    46081 tctggcggcg ctgtatgaaa gctggtcggt gccgttctcg gtaatgctgg tcgtgccgct
    46141 gggggttatc ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg acgtttactt
    46201 ccaggtgggc ctgttaacgg tcattggttt atcggcgaaa aacgccatcc tgatcgtcga
    46261 gtttgctaac gagatgaacc aaaaaggcca cgacctgttt gaagcgacgc tccacgcctg
    46321 ccgtcagcgt ttacgcccga ttctgatgac ctcgctggca tttatcttcg gcgtattgcc
    46381 aatggcaacc agcacgggtg ccggttccgg tggtcagcat gcggtgggta ctggcgtaat
    46441 gggcgggatg atttcggcca ctattctggc tatttacttc gtgccgctgt tctttgtgct
    46501 ggtgcgccgc cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa aggcgacatg
    46561 ccaatgtgtc gcctttttca actttccgat taagaacctg ctcagcaggt tcttgctgtt
    46621 tgtactttgt ctcaggaatt acttacgaag cataacttcg ataaagtctt tccagttccc
    46681 cagttcacgt tcaatcataa caacctctct tataattatg ggtattctac ggaaacaata
    46741 taccgtggtg aagctaattt actcgattgc tgcgatgact acctccgggg gacaaacctt
    46801 atgtaaatac tatggtcctg cggtgatgat ttg
//
LOCUS       DAOTRK010000016        44790 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.016, whole genome shotgun sequence.
ACCESSION   DAOTRK010000016 DAOTRK010000000
VERSION     DAOTRK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.016"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..252)
                     /locus_tag="QIE41_000991"
     CDS             complement(<1..252)
                     /locus_tag="QIE41_000991"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HDS3529905.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGTSGTEEIYFAT"
     gene            complement(354..1790)
                     /gene="cpsB"
                     /locus_tag="QIE41_000992"
     CDS             complement(354..1790)
                     /gene="cpsB"
                     /locus_tag="QIE41_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDS3529906.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(1793..3016)
                     /gene="wcaI"
                     /locus_tag="QIE41_000993"
     CDS             complement(1793..3016)
                     /gene="wcaI"
                     /locus_tag="QIE41_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDS3529907.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADVDV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGICQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(3013..3492)
                     /gene="gmm"
                     /locus_tag="QIE41_000994"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(3013..3492)
                     /gene="gmm"
                     /locus_tag="QIE41_000994"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDS3529908.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPEALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(3495..4460)
                     /gene="fcl"
                     /locus_tag="QIE41_000995"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(3495..4460)
                     /gene="fcl"
                     /locus_tag="QIE41_000995"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDS3529909.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(4463..5584)
                     /gene="gmd"
                     /locus_tag="QIE41_000996"
     CDS             complement(4463..5584)
                     /gene="gmd"
                     /locus_tag="QIE41_000996"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDS3529910.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(5611..6159)
                     /gene="wcaF"
                     /locus_tag="QIE41_000997"
     CDS             complement(5611..6159)
                     /gene="wcaF"
                     /locus_tag="QIE41_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDS3529911.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(6175..6921)
                     /gene="wcaE"
                     /locus_tag="QIE41_000998"
     CDS             complement(6175..6921)
                     /gene="wcaE"
                     /locus_tag="QIE41_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDS3529912.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTCEYLENLKGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(6932..8149)
                     /gene="wcaD"
                     /locus_tag="QIE41_000999"
     CDS             complement(6932..8149)
                     /gene="wcaD"
                     /locus_tag="QIE41_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDS3529913.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(8124..9341)
                     /gene="wcaC"
                     /locus_tag="QIE41_001000"
     CDS             complement(8124..9341)
                     /gene="wcaC"
                     /locus_tag="QIE41_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDS3529914.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKKGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAVFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(9338..9826)
                     /gene="wcaB"
                     /locus_tag="QIE41_001001"
     CDS             complement(9338..9826)
                     /gene="wcaB"
                     /locus_tag="QIE41_001001"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDS3529915.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(9829..10668)
                     /gene="wcaA"
                     /locus_tag="QIE41_001002"
     CDS             complement(9829..10668)
                     /gene="wcaA"
                     /locus_tag="QIE41_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDS3529916.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAITLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(10797..12959)
                     /gene="wzc"
                     /locus_tag="QIE41_001003"
     CDS             complement(10797..12959)
                     /gene="wzc"
                     /locus_tag="QIE41_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDS3529917.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSFAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(12962..13405)
                     /gene="wzb"
                     /locus_tag="QIE41_001004"
     CDS             complement(12962..13405)
                     /gene="wzb"
                     /locus_tag="QIE41_001004"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDS3529918.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(13411..14550)
                     /gene="wza"
                     /locus_tag="QIE41_001005"
     CDS             complement(13411..14550)
                     /gene="wza"
                     /locus_tag="QIE41_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDS3529919.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            15209..16792
                     /locus_tag="QIE41_001006"
     CDS             15209..16792
                     /locus_tag="QIE41_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDS3529920.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RVVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            complement(17244..19097)
                     /gene="asmA"
                     /locus_tag="QIE41_001007"
     CDS             complement(17244..19097)
                     /gene="asmA"
                     /locus_tag="QIE41_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDS3529921.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRINRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEQPEWQLRLQFPQLNLDNLIPLNETANGENGTAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(19119..19700)
                     /gene="dcd"
                     /locus_tag="QIE41_001008"
     CDS             complement(19119..19700)
                     /gene="dcd"
                     /locus_tag="QIE41_001008"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDS3529922.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(19792..20433)
                     /gene="udk"
                     /locus_tag="QIE41_001009"
     CDS             complement(19792..20433)
                     /gene="udk"
                     /locus_tag="QIE41_001009"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDS3529923.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            20751..24068
                     /locus_tag="QIE41_001010"
     CDS             20751..24068
                     /locus_tag="QIE41_001010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDS3529924.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNNTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAATHSERAA
                     ISLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(24106..24963)
                     /gene="alkA"
                     /locus_tag="QIE41_001011"
     CDS             complement(24106..24963)
                     /gene="alkA"
                     /locus_tag="QIE41_001011"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDS3529925.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLSMTIPGDVEQAMKMLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            25097..26449
                     /gene="yegD"
                     /locus_tag="QIE41_001012"
     CDS             25097..26449
                     /gene="yegD"
                     /locus_tag="QIE41_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDS3529926.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLV
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(26462..28402)
                     /gene="yegI"
                     /locus_tag="QIE41_001013"
     CDS             complement(26462..28402)
                     /gene="yegI"
                     /locus_tag="QIE41_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDS3529927.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQSTLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATIHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYTYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGMATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQTEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEILGLPEEEKRDLAALQDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKVALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPVDRQAVMAKMTAKRHQLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSCC"
     gene            complement(28399..29160)
                     /gene="pphC"
                     /locus_tag="QIE41_001014"
     CDS             complement(28399..29160)
                     /gene="pphC"
                     /locus_tag="QIE41_001014"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDS3529928.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDDDAVSRLETYTSTERAHKVAAFTDGIQRLALNMLNNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(29157..29816)
                     /locus_tag="QIE41_001015"
     CDS             complement(29157..29816)
                     /locus_tag="QIE41_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDS3529929.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVCQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(30037..30096)
                     /gene="ibsA"
                     /locus_tag="QIE41_001016"
     CDS             complement(30037..30096)
                     /gene="ibsA"
                     /locus_tag="QIE41_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDS3529930.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(30369..30425)
                     /locus_tag="QIE41_001017"
     CDS             complement(30369..30425)
                     /locus_tag="QIE41_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDS3529931.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(30697..30753)
                     /locus_tag="QIE41_001018"
     CDS             complement(30697..30753)
                     /locus_tag="QIE41_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDS3529932.1"
                     /translation="MMKLVIIVVLLVISYPAY"
     gene            31032..32279
                     /gene="mdtA"
                     /locus_tag="QIE41_001019"
     CDS             31032..32279
                     /gene="mdtA"
                     /locus_tag="QIE41_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDS3529933.1"
                     /translation="MKGSYKSLWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDITTVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            32279..35401
                     /gene="mdtB"
                     /locus_tag="QIE41_001020"
     CDS             32279..35401
                     /gene="mdtB"
                     /locus_tag="QIE41_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDS3529934.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            35402..38479
                     /gene="mdtC"
                     /locus_tag="QIE41_001021"
     CDS             35402..38479
                     /gene="mdtC"
                     /locus_tag="QIE41_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDS3529935.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            38480..39895
                     /locus_tag="QIE41_001022"
     CDS             38480..39895
                     /locus_tag="QIE41_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529936.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVSTT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            39892..41295
                     /gene="baeS"
                     /locus_tag="QIE41_001023"
     CDS             39892..41295
                     /gene="baeS"
                     /locus_tag="QIE41_001023"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDS3529937.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            41292..42014
                     /gene="baeR"
                     /locus_tag="QIE41_001024"
     CDS             41292..42014
                     /gene="baeR"
                     /locus_tag="QIE41_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDS3529938.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            42194..42526
                     /locus_tag="QIE41_001025"
     CDS             42194..42526
                     /locus_tag="QIE41_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDS3529939.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            42674..44035
                     /gene="yegQ"
                     /locus_tag="QIE41_001026"
     CDS             42674..44035
                     /gene="yegQ"
                     /locus_tag="QIE41_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDS3529940.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     VEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEQMENAKGKAMPVAPGDGYTVWIPVPQDLELNYALLMRNFSGETTRTP
                     HGK"
     gene            complement(44365..44682)
                     /locus_tag="QIE41_001027"
     CDS             complement(44365..44682)
                     /locus_tag="QIE41_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529941.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT        10371 a        12031 c        11577 g        10811 t
ORIGIN      
        1 cgtggcgaaa tagatctctt cagtgccgga cgtgccaata tccagcacat cgacgcccgc
       61 atcctgtaaa ccttttgcca gcgccagttt taaggtttcg ctggtgaggc ggacatcacc
      121 gcctaaaaca atggttttcg gtttaagaaa ttcgccatag gcgcgaccaa tacgccaggc
      181 aatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt
      241 taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg aagcggccaa
      301 ttttgttttt gtcgttattt accagagaat taatccggtt gattattcaa ctactacacc
      361 cgcccgtagc gatccgcgaa gcgcaccaca tcatcctctt cgagataaga gccggagcgc
      421 acttcaatta aatcgagcgg aattttcccc gggttttcca ggcaatgtgt cgcccccagc
      481 ggaatataaa tggactcgtt ttcaccaagc agtttgatat caccgtcaat agtgactttt
      541 gccgttcctg cgaccaccac ccagtgttcc gcgcggtgat gatgcatctg taccgacaag
      601 ccctcgcccg gtttcacggt gatgcgtttc acctggtagc ggtcgcccgc atcaatagag
      661 tcatatttgc cccacggacg atacacttca cgatgcaccc gatgctcatg gcgaccgtcg
      721 gctttgatct gctcgaccac ttttttcaca tcctgcaccg cgttacggtc ggcaatcagc
      781 actgcatctt tggtctgcac taccaccaaa tctttcaccc cgacggtggt gaccaggcca
      841 gattcggcat acacatagct gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg
      901 ttgccctcgg cggtgtgggc gctgatctcc cataatgaag accaggaacc gacatcgctc
      961 cagcccgcat ccatcggcac cacaacggca tctgccgtac gttccatgac cgcgtaatcc
     1021 accgactctt ccgggcaggc cagaaaggct tcttcatcca cgcgaataaa atcgagatcc
     1081 ggatcgacgg cgctcatcgc tttttcgcag gcatcgagga tatccgggcg atattttttc
     1141 agttcttcga gatagcgtcc ggcacggaac aggaacatac cgctgttcca gtaatattcg
     1201 ccgcttgcta cataggcctg cgcggtttcc agatttggtt tttcgacaaa ctgcgccact
     1261 tcaaaggcca ccgtatcctg ctcacccgca gacacttcac cgcgacgaat atagccatat
     1321 ccggtttcag gtagatccgg cacaatgccg aaggtcacca gcttgcccgc ttcggcatat
     1381 ggcatagcat tacgcacggc ggcacggaac gcgtcttcat cggcaatcac atgatccgcc
     1441 gccagcacca gcattaacgg gtcgctctcc gggctatgac gttttgccgc cagcgccgcc
     1501 agcgcaatgg cgggtgcagt gttacgccct gccggttcga gaataatgtt ctcggtgagt
     1561 ttgttcagtt gacgcagctg ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc
     1621 acagggcttt cgcactccac gccgttcagg cggcagatgg tggtttgcag catggtgaga
     1681 tcgcctttca ggcataaaaa ctgcttggga taaagtacgc gggaaagcgg ccataagcgg
     1741 ctaccggagc cacctgccat cacaactgga tagagtttcg actgcgccat aattatcccc
     1801 gaatatcatt tataaattga cgtaacacgt tctctttatc gagcgtgcgt tcggcatatt
     1861 cacgtgccac cgtgttgtgt ttgggcagca ggagcgcctg gcaaatcccc gccaccagcg
     1921 cctcgaccga ttccggctct acgcaaaccg caatgcccgg aaaggtttcg caaagctgcc
     1981 ccagttctgt gtgggcctct gcggtaatca ccgcgttacc gcctaccgcc agaatattgg
     2041 tcagtttcga cggcaatacg gcatctgccg cgccgcgttt ttgcaccacc agatggcaat
     2101 cgcccatctt cagcagtgcg ggtaaagcgt catacgattg cagcgggaaa aattgcatgt
     2161 tgcgcagtcc acgctgctgc gccatttttt ccagccgcgc tttgccgccg ccctgcccga
     2221 caatggcaaa aatcagcggt tcatcgcgca ggcgatcggc agcttcaata acgttttcca
     2281 gcccctgctt ttcaccaata ttgccggagt aaagaatgat ttttttgtta tccggcaggc
     2341 caagctggtt acgaagggca tcaacatcga catctgcgac atgctgaaaa cgggcaattt
     2401 ctgaccagtt gggaaagaag atgacgtttt ctgccgccac gcctttttcg atggctttat
     2461 tcatcatcga acgcgaaatc gtggagacgt tatcgacgtt atgcagtccg ctacgttcga
     2521 acgccgttgc cagctgtgcc actttgccgc ctttgccttt tccggcaagg cccagcccca
     2581 gcatggcgtc cacttcgtaa tcctgaatat gcagcacggt acgcgcgcca gagagtttcg
     2641 ccagcaggcg cattcccggc gtgcaaaaca gcgttggcac cacgccaata atgcgatccg
     2701 gcttccagcg acgttgcgcc atcaacggga aaaaactgct gacggcaaaa ctgccgagat
     2761 gcaacaagcg tttcagggtg ctcggctgtt ttggcacgta cagcgggcag cgccacaccg
     2821 tggcggcccc ctcttctcgt ttgtagcgcc aggcggaata gttctcgccc acctgccact
     2881 gcgggtagta aggcggtgcg gttatgaccc gcacctcatg accttgtgcc gcaagccatt
     2941 ccaccatctc tccggtgtat ttgccgatgc cggttaactc cggcgagtag ttaatgccgt
     3001 agaccagtat tttcataatc cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg
     3061 gctgttagcg tgaacattgt cactggcgag cagcgcttcc ggcgtcagcc agcggtaatc
     3121 gtcatgctgc tcatccggca gtaacagatc ttcttccgcg actctgaagc gaaaaccgag
     3181 caccacatag tgcgtggtga aatccgtgcc agagaagtta tcgtcataaa agtgctgcca
     3241 gacaccgtaa aactggcctg ctgttatcgg cagacgcagc cccagttccg ccatcgtcag
     3301 ccgctcaaat gcggcttcca gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca
     3361 gtaaccctgc gccgggcggt tggttctttt gccaagcaga aactcgccgc gactgttctc
     3421 aacaataaag tcgagagaga caagcggagt ggagcgcact accgtggcaa agtcttcctg
     3481 acgtaaaaac atcattaccc ccgaaagcgg tcttgattct caaggaacca ctggtaagtg
     3541 ctggcaagcc ccgcttccag tgagatttcg tgataccagc caagctgatg caggcgcgtc
     3601 acatccagca gtttgcgcgg tgtgccatcc ggtttgctgg catcaaaaac cacccgacct
     3661 ttgtaaccca ccactttggc gatggtttgc gccagctcgc ggatagtgca gtcaacgccc
     3721 gtgccgacat taatgtgcga cagcatcggc tgggtgttct ccagccagac ttcatgcgcc
     3781 agctccatca catgaatgct cgccgccgcc atatcatcga cgtgcagaaa ttcgcgcatc
     3841 ggcgtaccgc tgccccacac caccacgtcc ggcgcattct gtgccgtcgc ctcgtggaag
     3901 cgacgcagca atgctgggat cacatgcgag ttactcggat ggaagttgtc gtgcggcccg
     3961 tacaggttgg tcggcatgac cgagcggtaa tcgcgtccgt actggcggtt gtaagattcg
     4021 cacagtttga tcccggcgat tttggcaatg gcgtaaggct cgttggtcgg ctccagcgta
     4081 ccctgcaata actcgctttc tgccatcggc tgttttgcca gtttcgggta gatacaggac
     4141 gatccgagaa acagcagttt gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc
     4201 tcaatcatca tgttctgata gatgaaatcc gccggatagg tgttgttggc aacaatgccg
     4261 cccactttcg ccgccgccag atagacctgg tcaatgcttt cgctggcaaa gaaatcatgc
     4321 accgcgcggc tgtccagcag gttcagttca tcgcgggtgc gtaataccag ctccacatca
     4381 ccgcgctgtt cgagctgtcg cctgatggcg gaaccgacca ttccgcgatg gccagcgata
     4441 aaaatgcgtt gtttactcat gcttatgact ccagcgcgat cgccacgtcg tagccgtgag
     4501 atttcagcag agagtgtttt ttcgccgctt cgaggtcatt agccaccatt tcagacacca
     4561 tctctctgag ggtgatttcc ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgc
     4621 cgagcagcgt ttcaacttcc gccggacgga agtaacgcgg gtcaacggcg ataatcacat
     4681 cgcccggttt aacgcccggc gcatcatgcc cggtgacgga aaccacaatg cccttctctt
     4741 caacgcccgt gccttcaaag cgcagtttga tacccaactg tgccgccgcc atttctacga
     4801 actgacgcac ggagtactga acgccggtag caattacgaa atcttccggc tgttcctgtt
     4861 gcagcatcat ccactgcatt tttacgtagt ctttggcatg gccccagtca cgcagggaat
     4921 ccatattgcc gaggtacagg cacgactcca gcccctgggc gatgttggcg attgcgcggg
     4981 tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga ttcatggttg aagagaatac
     5041 cgttacaggc gtacatgccg taggattcgc ggtagttaac ggtgatccag taggcgtaca
     5101 gtttggcgac cgcatacgga gatcgcgggt agaacggcgt ggtctctttc tgcggaattt
     5161 cctgcaccag accgtacagt tcagaggtgg aagcctgata gaaacgggtt ttcttttcca
     5221 gaccgaggaa gcggatcgcc tccagcaggc gtagcgtacc catcgcatcg acgtctgcgg
     5281 tatattccgg tgactcaaaa gaaaccgcaa cgtggctcat tgcgcccagg ttgtacactt
     5341 catccggctg cacttcacgc aaaatacgcg tcaggttgga ggtatcactc aggtcgccat
     5401 aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct
     5461 cggtgttgaa tgacgatgca cgacgcttaa taccatgcac ctcgtaacct ttttccagca
     5521 gaaactctgc caggtaagaa ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg
     5581 acatgtctta ttcctctgta ttttttgaat ttattcagtt tcaacgcgtt cgcgtatcac
     5641 cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa cactgcttcg
     5701 cgcacccacg acggtgccgt cgccgatcgt gacgccaggg gcaacaaaaa catctgtcgc
     5761 cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat gttgacttgc
     5821 atggtcgtgg ctcccggtgc ataaataact tttttgcgat atcaccgaat gtgcgccaat
     5881 ggttatttca ccgagggtat ataaattgac gtcatcgccg acccacgcgt aatcacctaa
     5941 ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt tttttcctat
     6001 ttttgcgccg aataaacgta ataaaaatgc ccgccagcga tacaatactt gtggcgacca
     6061 ggcaaatatt gttgcctgta ctgcccacca caattgcact ttaatagcgt tgccaccccg
     6121 gaaccctttc ggcaccgaga atccacttaa atcctgcatc gttttccctt atattcagct
     6181 tttgttatat aaggctttcg tctttgaggt agtacgttgg cgtaaatgcc aggataattc
     6241 agcccaaaag ccaggcacgt gcaatatctg ccgttggact tttttggcgt cagcacacaa
     6301 ttccatatta ttggtggtag ataccccacc catggaaaat tcagacacca gaccattgag
     6361 ttttttaaat gcataaccgg ctttatacat tttggccgcc agcgcgtagt cggacgaaac
     6421 tttatattcc aggtcataac gccatttttt caagccggat accgggaaaa atatcgcctg
     6481 atgactggcg ggcaggctgt gataaatata ccagcccggt ttggcgctac gtttaatttt
     6541 atgcccgtcg ccaaaatcca gcagcgcatc gccagtgatc atcacgttat ctttttgtac
     6601 ttttaactta cggacaaaat ttgcggcatc ctgatgaaaa atatcacctg agttgagaaa
     6661 caacgcgaac ttgccttgtg ccatcgcaat gcctttgttc atggcgtcgt agataccgtt
     6721 atctggctcg ctgacaaagc gtaggttata gatacccttg agattttcca ggtactcaca
     6781 ggtgccgtca ttggaaccgc catcgacaac aatccattcg aagctgatat cgtccgcctg
     6841 cgccagatgc gccagcgagg cgtgtgtttt gactatccct gcgaggttac gaaacgcgac
     6901 agtgattatg ctaagcaaca tgttcttatt cttacctcgt aatatttaac gcttttcgca
     6961 aaataaacgg acagacgatt aaaaatgcat attccgggct aaatatcgaa ccggtaaaaa
     7021 acagcgatac cggagtaaaa agatataatt gcacgcgaaa attgcggtta tcaccaaaag
     7081 cgttgatcat catttttatc actttcccca tgtaccacag cgataaaaac actgcgaacc
     7141 aggaaaaata aataatcagc agatacaaac cattgtctat ggtttttccg acatccgcac
     7201 cgttaaatat tccgaatgat gcgacatatt cataaagtga gccaaatctg actacaccgt
     7261 caatatgggt caaagaataa ccgaccatca ccagcggacc gacaatacga taatacgatg
     7321 acgatccttc cgtgcctaaa tcacccagac gggtggaaat ataaggaaat gcaattacca
     7381 caccaaccag gaatacagcc agagaaatca atgctaacgg taactttttc ttaatcgcct
     7441 ctttattcag atattgaaat gcccactcca gcaaataaaa caggataaag gtcataaccc
     7501 ctgaaaacga tccggataat attatccctg cgagaatcat agcatcggtt ttaggcgttt
     7561 tgataccaaa ctgtttgatg ctgagccaaa ttgagattaa tgccagagcg aaaaatgccg
     7621 gttcgaaata aagtgctgtc gtgcgcttgc cgccgaattt gatgaaattc agcacatagc
     7681 tgttgctgta aatcagatat ttcgaaatcg attccataat actgctgccg ccggtgagga
     7741 taatttgcgc catctccacc gccgccagcg ccaccaccaa cccgaccacc agataaaaaa
     7801 agcgtaatat cttgcggtgg ttgtggggcg aaatcgtttt aaagcgaata ctccacacca
     7861 tgccaataat gatcacaata tagacaaaca gcatggttga agtgacgtat ttactggcat
     7921 ccaacgactg accaaacagg tagttaaacg ccgtgagtcc cgcgccaatc cccagggcaa
     7981 tcattaattt tttaacgctg atgcgctcta aaaacagcag taacaagaca ggtaaaaaag
     8041 tgacgatggt gatggggaaa ctttcaccaa gctgggcgat tttgacgtta accagcaggt
     8101 agatcagcgg cagcagcagg tagctacaga ttctgataga agttgacata ctcctccagc
     8161 atctgttgtc cactgtaggc ggcacggctg cgctggctga actcagccag cgtggtacca
     8221 aataccgcct gcgcgatttc cggtttgctt aactggacca gttgcagcac gtcttcttca
     8281 ctgacggttt taccgccgga tttttgcagc acttcccgcg ccgcgtcgct atgggtggca
     8341 atcaccggca cgccaatcga tagcgcctca cacaaaatta gcgggtagtt atcgacgcga
     8401 gaactgaata ccagcgcatc catctgattg agcgcgctca tcaacttgcg cttgtcagtt
     8461 tcaaagccgt gattaaccac gttgccagcg gtgaacggcg agaacttacc aaaggtatgc
     8521 agttcgattt tgtcgcccag cgccatcatc tcgcgtacca gttgctggtt agttttgccg
     8581 tcgtaacgca ggtcatgcgc caccaccgcg attttcggct tgccctgggt ttcgcgcacc
     8641 ggaggcaagt ccgccagaat cgcttcggtt gccatatcaa taccgttatt gataatccgg
     8701 caacgccctg gaccgtacag actattgaaa gcatcagcca catgctggct gggggaaata
     8761 aactgacagc ccagcgccag catctcgcgg aataactggc gtttgcccgc caccagttgg
     8821 tgcgcgcgat caatcttcac cggcggataa ttatttaagg tcgggcattt ctggcagccc
     8881 tttttccagc cttcacaacc gtcggtaaag gcgcagcgtc cggtaacgct ccagtgatcg
     8941 tgtaacgtcc agaccaaagt gatatccggt ttgtggtttt tcactttttc gcaaaagcgc
     9001 accacgctct taagattcaa ccagtagctg tgcagcacat gaaaatgcag gaccaccgga
     9061 cccggtgtgc gagtaatggt gcgatataac tcattgaaat tgccgaacag atcgcgatta
     9121 aacagacgaa acagggcaat gttcgccatc gcggtcatcc gcggcgtatg tttgatgacc
     9181 tgcggatagt tctgatggct gacgctctct ttgccgcctt tgccgtaacc gtagacaaaa
     9241 tgtgacgcca gcccctgttg cagcgcgcgc tggtggagat ctaacgccac acctgctgcc
     9301 ccaccttccg ccagtcgcac attaaattgc agaatattca tttaattacc ttcactcgcg
     9361 ctttttctcc caccaccagc gcgttgtccg ggacagagtc gagcaccacg ctgcccgcgc
     9421 ccacggtgac gttgttacca agcgtgatat caccaagaat aatgacgttg gcaccgagct
     9481 cgacgccgtt gccaatgtgt ggacatgcca tgttatcggc accacgattg ccgatagtga
     9541 cgccgtggcg aatggtaaaa tcatcccccg ctaccacgtt tttattgatc actacggcgt
     9601 aaccgtgatg gatagtaaag cggcggccaa tggtcgcggc agcctggatt tcataaccga
     9661 aaaagcattc ggtgataatg cgatacagca ccagcagcgg ggccgcccac agattgttga
     9721 ggacgttctt tttgcgccac accgaacaaa aatgagcggc gcgataggca agaaccatgc
     9781 agcacgggcg taaactccag ctgttggcgc gcagatcttc cagcatcttt agcgcccccg
     9841 aataccatca gccagacgtt tgccgttacg caccgacagc agcgtcagca aagtgcgcca
     9901 ggtcatgcgt ttattgcgga tctggtagag ggtaaacagc tgatattttt tgctggcacg
     9961 gtcgaatttg tctttgtgct tgcggtaaaa gtggaagtac ccggagaatt ttttcggtga
    10021 cgaggtgatc tgcatttcac cgtgattgat atgcaggatc tgcgtcgcct cttccacttt
    10081 ccacggttcg ccatactcca ccaccatccg caggaagatg tcgtaatcct gtgccgcttt
    10141 cagttcggta tcaaacaaac actctttgaa acgccatgcc caggtaaaga cctgattgcc
    10201 aatgatattc cgtttataga acaggcggcg tgaatacggc gatttgggat acagtggcaa
    10261 acttgccggt tgggaataga cttcgccctg gcaaacgtag tcgttagcgt acaaaaaggc
    10321 gtgagtgacc agttgctgtt tatgggcgag gaagacgctc agacggttgg gtgtccattc
    10381 atcatcatca tcgatcccgg tgatgtattc cccttgcgcc agcgtaatcg cctggttacg
    10441 taccgcgcac gccccgctgt taatatcgtt gtgaatgtag gtgatacgcg ggtcgttgag
    10501 ggcggtgacg tactgttgca gctgttccca ggaggtggag caatcatcca cgatgatcat
    10561 ctcccagtta ctgtagtcct ggcgcaggac cgattttatc gcccgaatcg ccagttgttg
    10621 gcggttccag gtcggcatat agattgaaat cagcggattg tctttcatag gttgttctcc
    10681 cggagtgtcg agatgttctg ccggtttata agcttgttta cgggttctgt tgccggatgc
    10741 ggcgtgaacg gaggtcggat aagatgcgtc agcatcgcat ccgacagcgg atctcgttat
    10801 ttcgcgtccg acttatattc gtattcgtaa tagccataat cctgatacgc gctggcgcgg
    10861 cggaagatgg agttgagaat cacccctttc accggaatac cattttgctc aaagcggctc
    10921 agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat taacgtggtt
    10981 ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg cgtatcaatc
    11041 aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg ttcgctcatc
    11101 aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa tttggcaaaa
    11161 gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatttc cgacaggcca
    11221 ttaacgttat tagtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc gcagtcgatc
    11281 aacaacaccc gtttattggt ctggctgatc accgccgcca ggttggcgca gacaaaggtt
    11341 ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc ctgcatcatc
    11401 gcgaagtgca gactggtacg cagactgcgg atggcttcaa tcgccagatc ggttggattc
    11461 cccaccgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt tttgacgcta
    11521 tcgcgcgctt tttgccactc cgacagcggg atgctggcat atacgctgat accgtgttct
    11581 tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag cacacccacg
    11641 atagagagca tcaggccgag gataattgcg ccgaggataa tcagcccttt cttcggtttc
    11701 agcacaccag gctgggtgat tgccgggtca acaatgcgca catcgccgac ggtgctggct
    11761 tcggtgattt tcagctcctg ttctttattc agcagttgca tatagacctg ctgcccagac
    11821 tcgacatcgc gggtcagacg gacaatttcc tgctgggttt tcggcatcgc cgttacgcga
    11881 ccattaagtt tggctttttc gtcttccagc gcctgccgtt tctccagcag cgtgcggtac
    11941 gccgggtgaa ctttggtgta cagcttggag atttccgctt ctttaaaggt cagttcgttc
    12001 aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttccag cggcagatca
    12061 acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc gaggcggcta
    12121 cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc cgccgatttg
    12181 cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc gcggatctgt
    12241 tcacgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc gttctccgtt
    12301 accgtcaggc tgttttgcag ttggttgatc atccccagcg tggagtattt ggtgacggta
    12361 aactcactcc ccgggctggc gtgaatagct tcaaccatca gcgtgacgcc ttcttttttc
    12421 agcatctggc ccgcttgccc acgggcgcta aagccgccat cgctgctcaa agtgtagttt
    12481 ttgtcatcaa gcacattaag cgtaaacacc tgatccgcca tctccttagg acggttaaag
    12541 gtggtcactt ttaccgtctc gttctggcgt cccatcaggc gatcccagcc cgcaccgaaa
    12601 atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac cgttttacca
    12661 agcaccaggc gcgaacgaat caactggatc tcggcgtccg atgcaggcgg tttgttggct
    12721 aatgccgatc cgatatcctg cactaacgaa ttgccgctgc tttgttcgat ttgtaccagt
    12781 gcgtcggcgc tgtatatcgg cgtagcgaag aaggtgtaaa ccacggcaca gagggcgaat
    12841 acggcagtga tgccaattac ccaccagcgc gcctcaatga cggtgccaac caggcgacca
    12901 atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt ttctgtcatt
    12961 cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt tcaagtaatg
    13021 tgtacaccgc tgcaaacgtt tcccggcttt tgcgatacgg atcggggatt tcacattcgt
    13081 tatcccagtg accaaacagc atcactttgc cgcgcatctc aggtgccatc tcgcataagc
    13141 gttcgatatg gcgcttttcc atggtcaaaa tcaggtcgta gttgcgacac aggcgacggc
    13201 tgatttgccg tgcacagtga ccttccagag acagttgatg ttctgcggcg acgctgatag
    13261 cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc actttcagct
    13321 ccgggtgata acgttgcagt aagcgttccg ccgtcgggga acggcaaata ttgccgacac
    13381 agacaactaa aatgttgtta aacatgacga ttaccagtta tgaatgtcgc tggctgtatc
    13441 cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac ggttccagcg
    13501 ggaaaccggt gcggtggtga catacaccac gtcataaggt tgcaggcgga attctgtcgc
    13561 catcaccagc gacgtggcat cggacatatc cagctggtag atattggcaa tcttgccgtt
    13621 acgcccaccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt tggaggtggt
    13681 catgtcgatg ccttcagcat taccaagggc ttcagtcagg gtcatgccgc taaagtccat
    13741 tttcagggtg ctctgtttct tcacttcacc catcacaaac actttcagat catcattacg
    13801 cggcacgtag agaatatcgc caggataaag cagtcgattc tgattgaggt cgccgttttg
    13861 catcagcgcc tgcaatgaaa tgcgttcttc acgaccattg tgtgttaaca ccacgttgcg
    13921 ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa tggtcagtgg
    13981 cacgttggtg atcgcctgtt ggccggattt attcacctgg ccggagatat aggctttttg
    14041 cgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg tcgctaagcg
    14101 cccggtaata tcactgcgga tttcagcgag cgttttcccc accacgtgaa ctttgccgat
    14161 atacggataa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc tggagctgcg
    14221 gtactgaccg gctggcgtgg tgagttccgg gtgatcccag acggtgacat taagaacgtc
    14281 ccccggcccg acgcgatact ggtaattcgc gatctcactt tccagcgtca tattggggcg
    14341 cgctacgttg ggtcgtgggc gtaattgatc aatcaggcgc ggggtcaacg gataaacatt
    14401 caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt tacccatcgt
    14461 cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg acaccaataa
    14521 tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc agagtaatta
    14581 tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt aattgaataa
    14641 aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat tgacagaata
    14701 attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt tagctaccaa
    14761 tacactgatt tagtttaatt tttaaaaccc tctcaacatg cagtcgttga tgagaaaggg
    14821 ttattacgga aattaacttc cgaatataag gtgacattat ggtaattgaa tattggctct
    14881 ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta acaattcggt
    14941 aattggctat ttttaagaat tatattaagt gtcattcaat atggttttta ggagtttctt
    15001 taggttgaca atatttaata tagtgtctcc acatgcgata tttcttaaat aatgttttat
    15061 tattaccact tttaattcag ggataatgtg aggttataaa cctcaaataa tgtgagataa
    15121 atagtgccgc aacattgcat ttttgccctg atttatccat gatcgaattg tgacatttgt
    15181 catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt ctatctgggc
    15241 cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc tggtctttat
    15301 tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg ttaccgggct
    15361 gctgctggcg atgttaatgc gcctgttact gctggcgtca atctcctggc tggtcaccct
    15421 gacacaaccg ctattcagct ttcgctcgtt tacctttagc gcccgcgact taatcatgct
    15481 gtttggtggt ttcttcctgc tgttcaaagc cacgatggag ctgaacgaac ggctggaggg
    15541 gaaagacagc aataacccca cgcaacgcaa aggtgcgaag ttctgggggg tggtgacaca
    15601 aattgtggta ctggacgcca tcttctcact cgactcggtg attaccgccg tcgggatggt
    15661 cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga tgttgatggc
    15721 cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc tctgcttaag
    15781 cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg tcattccgaa
    15841 aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcgctca atcagttggc
    15901 tatctttaac cgtcgacgtt ttctttccgc taaccagaca ctgcgccagc ggactaccga
    15961 agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg ccgaaaccgc
    16021 gtccatgctg gtggatcatg gtaaccagca gatctttaat ccgcaggaac ggcggatgat
    16081 tgagcgggta cttaatctta atcagcgtac cgtcagcagc attatgacgt cgcgccacga
    16141 tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc tggagcgaaa
    16201 tcagcatacg cgggtggtgg ttaccgacgg tgatgacgca gaagatttgc tcggtgttgt
    16261 tcacgttatc gacctgctac aacagtctct gcgcggcgaa ccgctcaacc tgcgggtgtt
    16321 gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttacctgccc tggagcagtt
    16381 ccgtaatgcc cgcacgcact ttgcttttgt agtggatgag tttggctcgg tggaagggat
    16441 tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg aagtggaaga
    16501 gatcgacgcc cgccatgata ttcagaaaaa tgcggacggt tcctggacgg cgaatggtca
    16561 tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa aacgtgaata
    16621 tcacaccatt gccgggctat tgatggaata tttgcagcgt attccaaaac ctggcgaaga
    16681 agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc gcgtgcagaa
    16741 ggtgcagatt attccgctgc ataaggatgg cgagatggaa tacgaggtgt gatggtatcg
    16801 gccattttac ccaatgcttc gtaacgcctt ataacttcag caatgaagcg ttgtctgtgg
    16861 tacatggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc gtttcctgta
    16921 ggccggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat gttacctcgc
    16981 gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg cgatttatcg
    17041 ccgggagccc gcgttgccag ggtggtggcg attgagccac cctggcacgt tcaccggctt
    17101 gagcgttcca tagtaacgag gaacatcaag tgaacggaac agccttcgat cttacatatt
    17161 cagaatataa tggtgccgcc acgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc
    17221 tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac atctttgcca
    17281 ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc ttcgtcctgt
    17341 agatgtttgc gcagtaactg atccacttgc agactgtaat tgagttgctg ccagttgcca
    17401 taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct ttccccgttc
    17461 cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc cagattcaac
    17521 ataccttccc ctgtaagcgc cagcacaggc gattgacctt gcatgtcgtt taacgtcacg
    17581 acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gacgcgttac gttatcgaag
    17641 ttttcagcgg ccttcacatc accaccatta cgttctaccg cttgctgaat catctgctgg
    17701 aagttcatcc cttccatgcg cgtgtcggtc atttcgacat gtgcctgtcc ttgccagttg
    17761 tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag tgacattttt
    17821 ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc aacgttttcc
    17881 agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt gcccggcagt
    17941 gaaacctgtc caccgttaag tttgccctgc agttgagtaa tttccagcag acccgacttg
    18001 ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac gttactggcc
    18061 tgcaacaaaa tatcagccgt aaagccttgc agtccctgat aggccggttc atcaatacgc
    18121 gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cttgctgcgc ggtaccgttt
    18181 tcaccgttcg ctgtttcatt aagcgggatg aggttgtcaa gattcagttg cggaaattgc
    18241 agtctcagct gccattccgg ttgctctgtc agcgtgacct gtgcttgccc gctcagcgta
    18301 ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga ctcctgccac
    18361 tgggcctgga aactcccctg cccctgaata ccttgttttg gtaaatctgc accctgcaac
    18421 tgccagttaa cttgttcaat agccgccgtt aaatcatgcg gataatcaga agcatctacc
    18481 gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taatccgccc ggagaactca
    18541 aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg gattgtcacc
    18601 tgctcgtcat cttcatgctg gaacaccagc acgctgtccg ccaccttaag actggatata
    18661 tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg tggtgcaacc
    18721 ggggcatctt cactgcgtac cgcttccgtc tgcggcgtca gttggatcac tgcccctttt
    18781 agcatcacct gcttaacgct cagttgatga ctcagcagtg gtaaaagcgc cacgtccaga
    18841 cgcatgttgt ctgcgcgaac cagtggctgg ctcgcgccct gggcggtgag agacattcgc
    18901 ccggagagga tactaagttg cggccagacg tgccaacgca gtggcccgtc gagctgcaat
    18961 tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc attcggattc
    19021 accagcaaca ctaacgcaga taacccggcc accagcacga ccaggagtat catcagcgtc
    19081 gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc gattcggctg
    19141 gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc
    19201 gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg acgcagcgcc
    19261 agcggcagct taccggagtt gtagaactcc agcacaatac agccagacca gcccggatcg
    19321 atacggtgcg cggtgacgtg caccataagc cccagacgcg ccagtgagga acgaccgtcc
    19381 agccatccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc cagcgccagc
    19441 tctcctgggt gaagataaaa cgcctcgctt tcatcgagta cgatctcatc gctcatcacg
    19501 cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa cgctgccgtg
    19561 tgaccacgga aggtacgaaa tttattgccc aggcgcacat ccaccgtcgc gccattaata
    19621 cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca ggcttcaata
    19681 tctcggtcac acagacgcat ggcattttct cctttcgtgc atcactccct taacgccaat
    19741 tacgtcaagg gcataccagg ttatcactga acggtacaca atttatcaag cttattcaaa
    19801 gaactgactt attttcgctt tcaatatatc gatggcgatg cggtttttcc cgccgcgcgg
    19861 cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac
    19921 ggttttttga tattgcgcca tcactgaatc cattgaacgc ccacgctcgt taacgtcacg
    19981 cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaattg agaagttaag
    20041 ttcatcacgc aaacgcgcat ccgtcagcag caaaatgcct tcgagaatga tgactttctt
    20101 cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt aaaccggcag
    20161 gtcaatggcc gagccgcgtt tcaatgcttg taaatgctca agcagcaggc tgtgatccat
    20221 cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat ggctttgatc
    20281 tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt gctcacgtaa
    20341 ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc cagcgatacc
    20401 gataatgacg cactgatgag actgatcagt catatattta gcgacctgat taacctggat
    20461 gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca tccgcctgat
    20521 accagtcgaa tagcgttgcc gcgcgctcag agtaattgtt gacgaataat tcccggtggc
    20581 aaattacgtt gttcagtttt aatgcaaggt aaaaattgtt atacgaagtt gcgcaaatta
    20641 tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt ttgtactagc
    20701 ataaacacag aaacgaatac tggcgacctg gtcttgcgga taaagcggta atgagcaaac
    20761 aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc agtttaggtt
    20821 tagtctcgtt tatctttacc cttttctcgc ttgagctttc gcagtttggc acccaactcg
    20881 ccccattgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat gccgggcgca
    20941 tgtggccggg gattgcgctg agctgctcgc tgggaaatat cgccgcatcc atcctgcttt
    21001 tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa gccgtggtcg
    21061 gggcagtgct actgcgtaaa ttgctgccgt ggtataaccc attgcaaaat ctggctgact
    21121 ggctgcgtct ggcactcggc agcgccattg ttccgcctct gttggggggt gttctggttg
    21181 tcctgctgac gcccggagac gatcctctca gggcattttt gatatgggta ctttcagaat
    21241 ccatcggcgc actggcactg gtgccgctgg ggttgttatt taaaccacac tatctgctgc
    21301 gccatcgcaa cccacggttg ctttttgagt cgctgctcac gttagccatc acactgacgt
    21361 taagctggct ttcgatgctg tatctgccgt ggccttttac tttcattatt gtgctgttga
    21421 tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt gatcttcctt accacggtga
    21481 tgatggtgtc actgatgatg gccgctgatc cctccctgct tgctacgccg cgtacatacc
    21541 tgatgagcca tatgccgtgg ctaccgtttt tgctgatcct gctgcccgcc aacatcatga
    21601 cgatggtgat gtatgccttt cgtgcggaac gcaaacacat ctctgaaagc gaaacccgct
    21661 ttcgtaacgc catggaatat tccgccatcg gcatggcatt agtgggcacc gagggacaat
    21721 ggctgcaatc caacaaagcg ctctgccagt ttctcggtta cagtcaggaa gaactgcgcg
    21781 gactcacctt tcagcagctg acctggccgg aggatctcaa taaagatctg caacaggttg
    21841 aaaagctgat cagcggtgaa ataaacacct attcaatgga aaaacgctac tacaaccgca
    21901 atggcgatgt tgtctgggcg ttgcttgccg tctcactggt acgccacacg gatggcacgc
    21961 cgctctattt tatcgctcag attgaagaca ttaacgagct aaaacgcacc gaacaggtga
    22021 atcagcaact gatggagcgc atcacgctgg ctaacgaagc gggcgggatt ggcatctggg
    22081 agtgggagct aaagccgaat atttttagct gggataagcg gatgttcgag ctgtatgaaa
    22141 ttcctccgca tatcaaaccg aactggcagg tgtggtacga gtgcgtgctg ccggaagatc
    22201 gccagcacgc cgaaaaagtg attcgcgatt cattgcaatc acgctctccc tttaaactgg
    22261 aatttcgcat taccgtgaaa gacggcattc gccatatccg cgccctcgct aaccgggtac
    22321 tgaataaaga aggcgaagtc gaacgcctgc tcggcattaa tatggatatg accgaagtga
    22381 aacagcttaa cgaggcattg tttcaggaaa aagagcgcct gcacattacg ctggactcca
    22441 tcggtgaagc cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccggtgg
    22501 cggagaagat gagcggctgg acgcaggaag aagcattagg tgttccgctc ctgacggtgt
    22561 tgcatattac ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct
    22621 cacgttccgc gattgaacaa gatgtggtgt tgcactgtcg gagcggcggc agctacgacg
    22681 tgcattatag tattacgccg ttaagtactc tggacggcag caatataggc tcggttctgg
    22741 tgattcagga cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc
    22801 atgatgcact gacgcatctc gccaatcgcg ccagttttga gaagcaactg cgtatcctgc
    22861 tgcaaacggt aaacaataca catcagcgac atgcactggt gtttatcgat cttgatcgct
    22921 ttaaagcggt gaatgacagc gccgggcacg ccgctggcga cgctttgttg cgcgaactgg
    22981 cgtcattgat gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc ggtggcgatg
    23041 aatttggtct gctgctgcca gactgtaatg tcgaaagtgc gcgttttatc gctacacgca
    23101 ttatcagcgc cgttaatgac tatcacttta tctgggaagg acgagtacat cgggtaggtg
    23161 ccagtgccgg gattaccttg attgatgaca acaatcatca ggcagctgaa gtgatgtcgc
    23221 aggctgatat cgcctgttat gcctccaaaa atggtggacg gggccgggtg acgatttacg
    23281 aaccgcagca agctgccaca catagcgaac gggcggcgat atcgcttgat gaacagtggc
    23341 ggatgattaa agaaaatcag ttgatgatga tcgcccacgg tgtcgcttcg ccgcggatcc
    23401 cggaagcacg taatttgtgg ctgatttcac ttaagctctg gagttgcgaa ggcgagatta
    23461 ttgatgaaca aacatttcgt cgtagcttca gcgatccggc acttagccat gctcttgacc
    23521 gacgggtatt ccacgatttt ttccagcagg ccgcaaaagc tattgccagt aaaggcttaa
    23581 gtatcgccct ccccctttcc gttgccggtt tgagtagcgc cacgctggtg aatgaactaa
    23641 ttgagcagct ggaaaatagc cctctaccac cacggttatt acatctgatt attccggcag
    23701 acgcgatttt agatcacgca gaaagcgtgc aaaaactgcg gctggcggga tgtcggatcg
    23761 tattcagtca ggtgggccgc gatctgcaaa tcttcaactc gctgaaagca aatatggcag
    23821 attacctgct acttgatggt gagttatgcg ccaatgtgca gggaaatttg atggatgaga
    23881 tgctgattac gatcattcag gggcacgctc agcggctcgg gatgaagacc atcgccgggc
    23941 cagtagtttt acccctggtg atggatacgc tttctggcat cggcgtcgat ctgatttatg
    24001 gcgatgtgat tgccgatgcc caaccgctgg atttgctggt gaatagcagt tatttcgcga
    24061 ttaactaagg cgtgcttccc catcgcctga tgcggcgtat caggcctaca attcgtccgt
    24121 tccgtctggt tgccagcctt ccgtatacca gatatgcaac agcgcataag aacgccaggg
    24181 cttccagcgt tcggcataac ggcggatttg tgccggtgtc attcccggaa atcgctgttt
    24241 aatcagataa tcatccggca ggaaaacatc tttcgcctgc cagccacgta aggcgaaata
    24301 attcgccgtc cagcgcccga tacccggaaa agtttgcagc attttcatcg cctgctccac
    24361 atcgcccgga attgtcattg ataaggtgcc ctccagcgcc gcatttgcca gatgaatcag
    24421 tgcctctgcc cgtttcaacg gcatacctaa cgcttttaat gcctgcgggt cggctgctgc
    24481 cagccgctga ggcgtgggga agcagacata atccggaaaa tcatccagcc gttcgccata
    24541 aagctgtgcc actctggcgg tcaattttgc cgccatcgcc acgctcacca gttggcctaa
    24601 aatcgcccgc acgccctgct caaaagcatc aacacaacca ggtaaacgca atccgggccg
    24661 cgccgcgcct aacttgccca acgcaccgtt aacaatctgc gggttacatt gcagatcaaa
    24721 caggcggctc attttcgcca gacactctgc ggcaacaggt tctaaacctg cacttaaatt
    24781 tatgtgcaga gtatggcggg ctatatccgg aatagcagtc accacgccgc gatattcgcc
    24841 caccgccaga ctacgggcat aataatcgtc agcgaccgtt tccacaccgt tcaccgcacg
    24901 ggcggcgaga aatcccaaca tccacgacca gtcatacggc ggctgccagt tcagggtata
    24961 catcgcatct ccttgttaat ccgctttcag cataaacgtt attcagacgc tacgctttgc
    25021 tttcatattc cggttgtcgc gacggcaaca tttcgctaaa gtcacgcccc ttcttcaccg
    25081 gcatggggat tatttcgtgt ttattggttt tgattacggt acagcaaact gttcagtggc
    25141 ggtcatgcgc gacggtaagc cgcaattact gaaaatggaa aacgacagca cgctgctgcc
    25201 ttcaatgctt tgcgcgccaa cgcgtgaagc ggtaagcgaa tggctgtacc gccatcatga
    25261 tgttccggca gacgacgatg aaacgcaggc gctgctgcgt cgggcgattc gttataaccg
    25321 cgaagaagat atcgatgtta cggcgaaaag cgtgcagttc ggtctttcct cactggcaca
    25381 gtacattgat gatccagaag aagtgtggtt tgtgaaatca ccaaaatcgt tcctcggtgc
    25441 cagcggctta aaaccgcagc aggtagcgct gtttgaggat ctggtctgcg caatgatgtt
    25501 gcacattcgc cagcaggcgc aggcacagct gccagaagcg attactcagg cggtgattgg
    25561 tcgccctatc aacttccagg ggctgggcgg cgatgaagca aacacccagg cgcaggggat
    25621 tctggaacgc gcggcgaagc gtgccgggtt taaggatgtg gtattccagt acgagccggt
    25681 cgcggctggg ctggattacg aagccacctt gcaggaagaa aaacgggtgc tggtggtgga
    25741 tatcggcggt ggtacgactg actgttcatt gctgctgatg gggccgcagt ggcgttcgcg
    25801 tctcgatcgt gaagccagcc tgctgggtca cagtggttgc cgtattggcg gtaacgatct
    25861 ggatatcgca ctggcgttta aaaacctgat gccgttactg ggcatgggtg gcgaaaccga
    25921 aaaaggcatc gccctgccga ttctgccgtg gtggaatgcg gttgccatca acgacgtacc
    25981 tgcgcaaagt gatttctaca gtagtgccaa cggtcgtctg cttaacgatc tggtacgcga
    26041 tgcccgcgaa ccggaaaaag tggccctgtt acagaaagtc tggcgtcagc gtttaagcta
    26101 tcgcctggta cgtagcgcag aagagagcaa aattgcgctt tcaagcgtag cggaaacccg
    26161 cgcctcactg ccgtttatca gcgatgaact ggctacgctg attagccagc aagggctgga
    26221 aagcgccctc aaccagccac tggcgcggat tctggaacag gtgcaactgg tgctggataa
    26281 cgcccaggaa aaaccggatg ttatctatct gaccggcggt agcgcccgtt caccgctgat
    26341 taaaaaagcg ctgacagaac agttgccggg cattccgatt gcaggtggcg atgactttgg
    26401 ctcagtcacc gccgggctgg cacgctgggc ggaagtggtg tttcgttaat tgaaaactgg
    26461 ctcagcaaca gcttaaatca gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg
    26521 gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc aactctgtcg
    26581 caccgacagt cagcgccgat tccagctgat gtcgtttggc ggtcattttc gccattaccg
    26641 cttgtctgtc tactggcgtt accgcttcat tcggcctgaa cacaaaacgg cgttcgcaac
    26701 ttgctttcca gtcgataact gcctgggtca gatgatcacc aaaacctcta acttgcttaa
    26761 cgctccgtcg ggtaacgtct gccgccgttt caataccaaa ggaccgtaac gccactttac
    26821 gcgcagggcc aacgccggga atagaggcaa catcaataaa aaatccctcc agaaacttct
    26881 gcttctgccg ttcccttgcg gtgtcctgaa gtgctgccag atcgcgcttt tcttcttccg
    26941 gtaacccaag aatttcgtcc ttcatttttt cgagcatcgt ccgtttggct ataaaacctt
    27001 ccagcccgcc caactgttgg atctggctga ctaaatggtc atagtccatt ttcgcgcgat
    27061 taaacgcctc acggcgttgc tggatttctg ctttgtacgc tttgcttgtc aggctgccaa
    27121 taatccatat agccgacagc acaggaacca aaataatata acgcggttct gtctgaaggc
    27181 cgcaaagcaa cagcgataac gctgacagtg cgatctcaat cagaatgatg tattcacgcc
    27241 ttaaaaggcc taaaggaagc ggcctgccag tcggttgaaa atgatcgggt aatggcagtt
    27301 gcaatgctgg tggtgctact gacgccatca ccatcgccca gactttcgcc agcacaaaat
    27361 caccgctggt ggtaatgacc tcttcgccga gatcaataaa atagataacg ccttgattat
    27421 ccagcgtaca ccacgggcag tcagtcaaat gaccggggta aacatgcatt gccgaaacgg
    27481 tacatttctt taactgttgg cgtagtaaat caagtgccgc tacccacgct tttgccgtcg
    27541 gacgcccggt tgccatgcca ctttccgtga atgcctgctg aaacatggct tcaacatcac
    27601 ccggtaacat cgacagcgga atagatcgtg gcggcggttt taaaccacgt cgctgattat
    27661 ctgacgcgta ggtataacgg aaatgggcaa tatccgtctc cagcgcatta cccgcatcag
    27721 agataagcgg tacaccggaa taaggatgcc gaccaccgaa caagacgtga aaaatcagca
    27781 acgcaaggcc aaaattatcg tgattcgcgg tgcgttcaaa gccgacaaat gatggcaagg
    27841 tttgcagctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc agtgtgccat
    27901 tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac tttgctgtca cggcctacca
    27961 taaagctgtt ctggtttacg tcacccacga cgtgcccgtg ctcgtgaatc gtagcaaaag
    28021 atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga gggtaactct
    28081 ggcgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa acttttggca
    28141 tcataaaacc gataactttt ccgcctcgtc caccgtgaag tgttgactgc ggccaggcga
    28201 cataattcaa caactgcgcg tcagctgtcg cagccataaa ggcaagtttg tcctgtttta
    28261 aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactcc tcgatatcat
    28321 aaaccgcacc ttcaccgcct ttgcccagtt cacggcctgg cgtaacgcac tcaccagtag
    28381 cagtatataa agtgggtttc atggtaacca cagcgccagt gccaacgttt tatcatcatc
    28441 ggtacgctcg tttaccgccg ggctggacaa aaactgcttt aacaattcag gtaataaatc
    28501 gagttgttcc tgcgttgctg acgccagtcc attgaaaaac ggggtaaaaa aaggcacatg
    28561 cggagaatta ttcaacatat ttaacgccag ccgctgaata ccatccgtaa atgctgcaac
    28621 tttatgcgca cgctcagtac tggtgtaagt ttccagtctg gaaacagcat cgtcatcggt
    28681 aatgaagtgc gtcatgttgg catattcacc caccatcggc gtgagaggca attgcagacc
    28741 gtgacccaaa tctaccacca cgccgccatc gccaatctgc ataatcaagg tgccattagc
    28801 tgatgaaatc aatcccaaaa acgtgcaggc aaaatcgcga accgctaatt ctttggcttc
    28861 cgcctcggca aaaagtcgct gacgaatggt cagcacaatg tccgttgcga ggacatcatt
    28921 aagccccaat tccccgccct gcactttttg cgacatataa gccatcgctt cattgacggc
    28981 gagcatcgca ccttcgccac cctgcgagac gctgcctgcg ccatcagcga caaataccga
    29041 caataatggc tgctggtcat ttaaccaggc aacctgcatc tggcaggcat cctggcaagg
    29101 caagtccgcg ctgatgtgcg acgttccaac ggttgatgca tagaccagac gccagctcac
    29161 actgacgtcc agccttttgg cgcttccagc acaacttccg ttcccggcgt ggagcgggaa
    29221 accgaacgca gcgaactgga taaccagcta aacagttcac ggaattgtaa tccctgcagt
    29281 ggcaaaggct gacaaacgct gatttgtgcc aaagtcttca tatccgcccc ctgaacaccg
    29341 atggaaaaga aggcaaactt cttatcttct tccccctgaa acactttgtt ggcggcagcc
    29401 tgccactcat cggttggagc gccatcggtg atcaggaaaa tccacgggcg ataataggaa
    29461 ataccattgg cgcgatactc acgtttgcgc tcctcaacca tatccagggc tttggtaatg
    29521 gcggccccca ttggtgtatc gccctgggca aacaggatgg gcgggaaaaa attagcggcg
    29581 ctggtaaagg gttgttccac atgcaccggg ccgaacgtca caataccgag ttccactctt
    29641 tttaaggcca gcgaatcggc aagcagctca tcgcgaaagg taaccaatcc ggcgttaagt
    29701 tcattaatgg gtctaccgtt catagaacca gaaacatcca gcagcaaaat gcaggggcaa
    29761 cgtggttccg ggttactggc aaaatcgctg gtggcaaacg tgatttgttc gctcataaga
    29821 aatcctttct tagagaatta ttgaggtccg gtatcatacc gactgcaaca atgaaatatg
    29881 agcaggcaaa gcaactttca gaattaggcc cggcggcgca gaaaattttg ctattcgcct
    29941 cgcaagaacg gagagccgtc gattgacact aagggcggag tgacataatt tcaggagtga
    30001 gggttaggga gaggtttccc cctccccctg gtgttcttag taagcctgga agctaatcac
    30061 taagagtatc accagtatga tgacgtgctt catcataacc ctttccttat taaaagccct
    30121 cttctccggg agagggtttc ccgtttcagc gtcccgctga aatcgtcggc ttacctcctt
    30181 tcgccctgaa tgcagtctat cgctaacgcg tgaacaaaat agtttcctct gtgattattg
    30241 cgtaacgact cgcaaagaat atttgctggc aggatcgcag actgcaaagc ctgcagattg
    30301 acaatcttat cgtgaaggca taatttcagg agtgagggta gagcggggtt tcccccgccc
    30361 tggtagtctt agtaagcggg gaagcttatg actaagagca ccacgatgat gagtagcttc
    30421 atcatgaccc tttccttatt taaggcccct tcctcgggag gggctttccc gtttcagcgt
    30481 cccgctgaaa tcgtcgactt acctcctttc gccctgtatg cagtctatcg ctgacttgcg
    30541 gataaaatag tttccagtgt gattattgcg taacgtctcg caaagaatat ttgctggcag
    30601 gatcgcagac tgcaaagcct gcagattgac aatcttatcg tgaaggcata ctttcaggag
    30661 tgagggtaga gcggggtttc ccccgccttg gtagtcttag tatgctgggt agctaatcac
    30721 taagagcacc acgatgatga ctaacttcat catgaccctt tccttattta tggccccttc
    30781 ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc
    30841 catgcaagca gtctatcgct aacgcgtaga taaaatagtt tcctgtgtta ttactggatg
    30901 cgtgctcgca aatgtgcccg ccattcagac gattcccgat agtgtttcat aattcctcca
    30961 tttttctccc ttattggctg gctacactag tatcattccg cgaaacgttt caggaagaga
    31021 aactcttaac gatgaaaggt agttataaat ccctttgggt aatcgtaatc gtggtggtta
    31081 tcgccgccat cgccgcattc tggttttggc aaggccgcaa tgactcccgg agtgcagccc
    31141 caggggcgac gaaacaagcg cagcaatcgc cagcgggtgg tcgccgtggt atgcgttccg
    31201 gcccattagc cccggttcag gcggcgaccg ccgtagaaca ggcagttccg cgttacctca
    31261 ccgggcttgg caccattacc gccgctaata ccgttacggt gcgcagccgc gtggacggcc
    31321 aactgatagc gttacatttc caggaaggcc agcaggtcaa agcaggcgat ttactggcag
    31381 aaattgaccc aagccagttc aaagttgcat tagcacaagc ccagggccaa ctggcaaaag
    31441 ataaagccac gcttgccaac gcccgccgtg acctggcgcg ttatcagcaa ctggcaaaaa
    31501 ccaatctcgt atctcgtcag gaactggatg cccaacaagc gctggtcagt gaaaccgaag
    31561 gcaccattaa ggctgatgaa gcaagcgtcg ccagcgcgca gctgcagctc gactggagcc
    31621 gtattaccgc accagtcgat ggtcgcgttg gtctcaagca ggttgatgtt ggtaaccaaa
    31681 tctccagtgg tgataccacc gggattgtgg tgatcaccca gacgcatcct atcgatttgc
    31741 tctttaccct gccggaaagc gatatcacta ccgtagtaca ggcgcaaaaa gccggaaaac
    31801 cgctggttgt tgaagcctgg gatcgcacca actcgaagaa attaagtgaa ggcacgctgt
    31861 taagtctcga taaccaaatc gatgccacta ccggtacgat taaagtgaaa gcacgcttta
    31921 ataatcagga tgatgcactg tttcccaatc agtttgttaa cgcgcgtatg ttagtcgaca
    31981 ccgaacaaaa cgccgtggtg atccccaccg ccgccctgca aatgggcaat gaaggccatt
    32041 ttgtctgggt gctgaatagc gaaaacaagg tcagcaaaca tctggtgacg ccgggcattc
    32101 aggacagtca gaaagtggtg atccgcgcag gtatttctgc gggcgaccgc gtagtgacag
    32161 acggcattga tcgcctgacc gaaggggcga aagtggaagt agtggaagcc cagagcgcca
    32221 ccactccgga agagaaagcc accagccgcg aatacgcgaa aaaaggagca cgctcctgat
    32281 gcaggtgtta cccccgagca gcacaggcgg cccatcgcgc ctgtttatta tgcgtcctgt
    32341 cgccaccacg ctgctgatgg tggcgatctt actcgccggg attatcggtt atcgtgccct
    32401 gcccgtttcg gcgctgccgg aagtggacta tccaaccatt caggtggtca ccctctaccc
    32461 aggtgccagc ccggatgtca tgacctctgc cgttaccgcg ccgctcgaac gccagttcgg
    32521 gcagatgtcc ggcctgaaac agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac
    32581 tttgcagttc cagctaacat taccgctgga tgtcgccgag caggaagtgc aggccgcgat
    32641 taacgctgcg accaacctgc taccgagcga tctgcctaac ccgccggttt acagcaaagt
    32701 gaacccggca gatccgccaa tcatgacgct cgctgtcacc tcaaccgcca tgccgatgac
    32761 gcaagtggaa gatatggtgg aaacccgcgt cgcgcaaaaa atctcgcaga tttccggcgt
    32821 cggcctggtg acgctttccg gcggccagcg tccggctgtt cgcgtcaaac ttaacgccca
    32881 ggcgattgcc gccctcggcc tgaccagcga aaccgtgcgc accgctatta ccggcgctaa
    32941 cgttaactcg gcaaaaggta gcctcgacgg cccttcccgt gcggtcacac tttccgcgaa
    33001 cgaccagatg caatccgccg aagagtatcg ccagctgatc atcgcctacc agaacggcgc
    33061 gccgattcgt ctgggcgatg tcgcaaccgt agagcaagga gcagaaaaca gctggctcgg
    33121 cgcgtgggcg aacaaagaac aggccattgt gatgaatgtt cagcgccagc ccggtgctaa
    33181 cattatctcc accgccgaca gcatccggca gatgctgcca caactcaccg agagtttgcc
    33241 gaaatcggta aaagtgacgg tgctttctga ccgcaccacc aacatccgcg catcggtcga
    33301 cgatacccag tttgaactga tgatggctat cgcgctggta gtcatgatta tctacctgtt
    33361 tttgcgcaat attccggcga ccattattcc tggcgtcgca gtgccgctgt cgttaatcgg
    33421 caccttcgca gtcatggtgt ttctcgattt ttcgatcaat aacctgacgc tgatggcgtt
    33481 aactatcgcc accggattcg tggtcgatga cgccatcgtg gtgatcgaga acatttcgcg
    33541 ctatatcgaa aaaggcgaaa aaccgctggc ggcggcgctc aaaggcgcag gtgaaatcgg
    33601 ttttaccatt atctcgctga ccttctcgct gattgcagtg ttgatcccac tgctatttat
    33661 gggcgatatc gtcgggcgac tgttccgcga atttgcaatt actctggcag tcgcgatttt
    33721 gatctcagcc gtggtgtcgc tgactctgac gccgatgatg tgcgcgcgga tgctcagcca
    33781 ggagtcgctg cgtaaacaga atcgcttctc ccgagcctcg gagaaaatgt tcgacaggat
    33841 aatcgccgcc tatggtcagg gacttgcgaa agtgctgaat catccgtggc tgaccttaag
    33901 cgtggcgctc agcacgctgc tgcttagcgt gctgttgtgg gtgttcattc cgaaaggttt
    33961 cttcccggta caggacaacg gcattattca gggcactttg caggcaccgc aatccagctc
    34021 ctttaccaat atggcccagc ggcaacgcca ggtcgcggat gtgattttgc aggatccggc
    34081 agtgcaaagt ctgacctcat ttgttggcgt tgatggcact aacccgtcgc tgaacagtgc
    34141 gcgtttgcag atcaacctca aaccgttgga tgaacgtgat gatcgggtgc aaaaagtcat
    34201 cgcccgtctg caaacggcgg tggataaagt gccgggcgtc gatctcttcc tgcaaccaac
    34261 gcaggacctg accatcgata ctcaggtcag ccgcacccag taccagttta ccttgcaggc
    34321 cacgtcactg gatgcgctca gtacctgggt gccacagttg atggaaaaac tccagcaact
    34381 gccacagctt tctgatgtct ccagcgactg gcaggatcaa gggctggtgg cgtatgtcaa
    34441 tgttgatcgc gacagcgcca gccgtctggg gatcagcatg gcggacgtcg ataacgccct
    34501 gtacaacgcg tttggtcagc ggctgatttc cactatttat actcaggcta accagtatcg
    34561 cgtggtgctg gaacacaaca ctgaaaatac cccaggcctc gcggcgctgg ataccattcg
    34621 cctgaccagc agcgacggcg gcgtggtgcc gctaagctca attgccaaaa ttgagcagcg
    34681 ttttgcgccg ctctccatca accatctgga tcagttcccg gtaacgacta tctcctttaa
    34741 cgtgccggat aactattcgc tgggcgatgc ggtgcaggcg attatggaca cagagaagac
    34801 gctgaatctg ccggtggata tcaccacgca gttccagggc agcaccctcg ccttccagtc
    34861 ggcattgggc agcactgtct ggctgattgt cgcggcggtg gtggcgatgt atatcgtgct
    34921 cggcattctg tacgagagct ttattcaccc gatcaccatt ctctcgacgc tacccaccgc
    34981 aggggtcggc gcactgctgg cgttaatgat tgccggtagc gagctggatg tgattgcgat
    35041 tatcggcatt attttgctga tcggtatcgt gaagaagaac gccatcatga tgatcgactt
    35101 cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc gaggcaatct accaggcttg
    35161 tctgttgcgt tttcgtccga tcctgatgac cactctggcg gctctgcttg gcgcactgcc
    35221 gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt ccgttaggta tcggcatggt
    35281 cggcggtctg attgtcagcc aggtgctgac gctgtttacc acgcctgtga tttatttgct
    35341 gttcgaccgc ctggcattgt ggaccaaaag ccgctttgct cgtcatgaag aggaggcgta
    35401 agtgaagttt tttgccctct tcatttaccg cccggtggcg acgattttac tgtcggtcgc
    35461 cattaccctg tgcggcatac tgggcttccg tatgctgccg gtcgccccac tgccgcaggt
    35521 cgattttccg gtgattatgg tcagcgcctc gctgcccggt gcgtcaccag aaacaatggc
    35581 gtcttccgtt gccacgccgc tggaacgttc gcttgggcgc attgccgggg tcagtgaaat
    35641 gacctccagc agttcgctcg gcagcacgcg cattattttg cagtttgatt ttgaccgcga
    35701 tatcaacggc gcagcgcgcg atgtgcaagc ggcgatcaac gccgcgcaaa gtttgctgcc
    35761 cagcgggatg cccagccgtc cgacctatcg caaagcgaac ccgtcggatg cgccaattat
    35821 gatcctgacg ctaacatccg atacttattc gcaaggtgaa ctgtacgatt ttgcctcgac
    35881 gcagctggct ccgacgattt cgcaaatcga cggtgtcggt gatgtcgatg ttggcggcag
    35941 ctcgctacct gcggtgcgcg tcgggctgaa cccgcaggcg ctgtttaatc agggcgtctc
    36001 gctggacgac gtacgcactg ccatcagcaa tgccaacgtg cgtaaaccgc aaggggcgct
    36061 ggaagatggc actcaccgct ggcagatcca gactaacgat gaactgaaaa ccgccgctga
    36121 atatcagccg ctgattattc attacaacaa cggcggcgcg gttcgtctgg gcgatgtagc
    36181 gacggtgacc gactcagtgc aggatgtgcg caacgccggg atgaccaacg ccaaaccggc
    36241 tattttgctg atgatccgca agctgccgga agccaatata attcagacgg ttgacagcat
    36301 ccgggcaaaa ttaccggagt tgcaggagac cattccggcg gcgattgatc tgcaaattgc
    36361 ccaggatcgc tcccccacta ttcgcgcctc gctggaggaa gtcgagcaaa cgctgattat
    36421 ctcggtggcg ctggtcattc tggtggtctt tttattcctg cgctcgggtc gcgccactat
    36481 tattcccgcc gttgcggtgc cggtttcgct gattggtacg tttgcggcga tgtacctgtg
    36541 cggtttcagt ctcaataatc tttcgttaat ggcgctcacc attgctactg gtttcgtggt
    36601 ggatgacgcc atcgtggtgc tggaaaacat tgcacgtcat ctggaagcgg gaatgaaacc
    36661 gttgcaagcc gcactgcaag gtactcgcga agtcggtttt acggtgctgt cgatgagtct
    36721 gtcactggtg gcggtgttcc tgccgctgct gttgatgggc ggattgccgg gccgactgtt
    36781 acgcgagttt gccgtgacgc tttctgtcgc cattggtatt tcgttgctgg tttccctgac
    36841 gctaacgccg atgatgtgtg gctggatgct gaaagccagc aagccgcgcg agcaaaagcg
    36901 actgcgtggt tttggtcgca tgttggtagc cctgcaacaa ggctatggca agtcgctgaa
    36961 atgggtgctc aatcataccc gtctggtggg cgtggtgctg cttggcacca ttgcgctgaa
    37021 tatctggcta tatatctcga tcccgaaaac cttcttcccg gagcaggaca ccggcgtgtt
    37081 gatgggcggg atacaggcgg atcagagtat ttcgtttcag gcgatgcgcg gtaagttgca
    37141 ggattttatg aaaattatcc gtgacgatcc ggcggtggat aatgtcaccg gctttaccgg
    37201 cggttcgcgg gtgaacagtg ggatgatgtt tatcaccctc aagccacgcg acgaacgcag
    37261 cgaaacggcg cagcaaatta tcgaccgtct gcgcgtaaaa ctggcgaaag aaccgggggc
    37321 gaatctgttc ctgatggcgg tacaggatat tcgcgttggt gggcgtcagt cgaacgccag
    37381 ctaccagtac acgttgttat ccgacgacct ggcggcactg cgcgaatggg agccgaaaat
    37441 ccgcaaaaaa ctggcgacgt tgccggaact ggcggacgtg aactccgatc agcaggataa
    37501 cggcgcggag atgaatctgg tttacgatcg cgacaccatg gcacggctgg gaatcgacgt
    37561 acaggccgcc aacagcctgt taaataacgc cttcggtcag cggcaaatct cgaccattta
    37621 ccagccgatg aaccagtata aagtggtaat ggaagtggat ccgcgctata cccaggacat
    37681 cagcgcgctg gaaaaaatgt tcgttatcaa taacgaagga aaagcgatcc cgctgtcgta
    37741 tttcgctaaa tggcaaccgg cgaatgcccc actatcggtg aatcatcagg gattatcggc
    37801 ggcctcgacc atctcgttca acctgccgac cgggaaatcg ctctcggacg ccagtgcggc
    37861 gatcgatcgc gcaatgaccc agcttggtgt gccttcgacg gtgcgcggta gttttgccgg
    37921 cacggcgcag gtgttccagg agacgatgaa ctcgcaggtg atcctgatta tcgccgccat
    37981 cgccacggtg tatatcgtgc tgggtatcct ttacgagagt tacgtacatc cgctgacgat
    38041 tctctccacc ctgccctcgg cgggcgttgg agcgctgttg gcgctggagc tgttcaatgc
    38101 cccgttcagc ctaatcgccc tgatagggat catgctatta atcggcatcg tgaagaaaaa
    38161 cgccattatg atggtcgatt ttgcgcttga agcccaacgg cacggtaacc tgacgccgca
    38221 ggaagctatt ttccaggcct gtctgctgcg ttttcgcccg attatgatga ctaccctggc
    38281 ggcgctgttt ggcgcgctgc cgctggtatt gtcgggcggt gacggctcgg agctgcgcca
    38341 acccctgggg atcaccattg tcggcggtct ggtaatgagc cagcttctga cgctgtatac
    38401 cacgccggtg gtgtatctct ttttcgaccg tctgcggctg cgtttttcgc gtaaacctaa
    38461 acaaacggta accgagtaaa tgacagatct tcccgacagc acccgctggc aattgtggat
    38521 tgtggctttc ggcttcttta tgcagtcgct ggacaccacc atcgtaaaca ccgcgcttcc
    38581 ctcaatggcg caaagcctcg gggaaagtcc gttgcatatg cacatggtca ttgtctctta
    38641 tgtactgacc gtggcggtga tgctgcccgc cagcggctgg ctggcggaca aagtcggcgt
    38701 gcgcaatatt ttctttaccg ccatcgtgct gtttactctc ggttcactgt tttgcgcgct
    38761 ttccggcacg ctgaacgaac tgttgctggc acgcgcgtta cagggcgttg gcggtgcgat
    38821 gatggtgccg gtcggcaggt tgacggtgat gaaaatcgta ccgcgcgagc agtatatggc
    38881 ggcgatgacc tttgtcacac tgcccggtca ggtcggtccg ctgctgggtc cggcgctcgg
    38941 cggtctgctg gtggagtacg catcgtggca ctggatcttt ttgatcaaca ttccggtggg
    39001 aattatcggc gcgatcgcca cactgatgtt aatgccgaac tacaccatgc agacgcggcg
    39061 ctttgatctc tccggctttt tattgctggc ggttggcatg gcggtattaa ccctggcgct
    39121 ggacggcagt aaaggtacag gtttatcgcc gctggcgatt gctggcctgg tcgctgttgg
    39181 cgtggtagca ctggtgcttt atctgctgca cgccagaaat aacaaccgcg ccctgttcag
    39241 tctgaaactg ttccgcactc gtaccttttc gctgggtctg gcggggagct ttgccggacg
    39301 tattggcagt ggcatgttgc cctttatgac accggttttc ctgcaaattg gcctcggttt
    39361 ctcgccgttt catgccggac tgatgatgat cccgatggta ctcggcagca tgggaatgaa
    39421 gcgaattgtg gtacaggtgg tgaatcgctt tggttatcgt cgggtactgg tgtcgaccac
    39481 gctgggtctg tcgctggtca ccctgttgtt tatgaccact gctctgctgg gctggtacta
    39541 cgttttgccg ttcgtcctgt ttttacaagg gatggtcaac tcgacccgtt tctcctccat
    39601 gaacaccctg acgctgaaag atctcccgga caatctggcg agcagcggaa acagcctgct
    39661 gtcgatgatt atgcaattgt cgatgagtat cggcgtcacc atcgccgggc tgttgctggg
    39721 gctttttggt tcgcagcatg tcagcgtcga cagcggcacc acacaaaccg tctttatgta
    39781 cacctggctt agcatggcgt ttatcatcgc ccttccggcg ttcatctttg ccagagtgcc
    39841 gaacgatacg catcaaaatg tagctatttc gcggcgaaaa aggagcgcgc aatgaagttc
    39901 tggcgacccg gtattaccgg caaactgttt ctggcgattt tcgccacctg cattgtcttg
    39961 ctgatcagta tgcactgggc ggtgcgtatc agttttgagc gtggctttat tgattacatc
    40021 aaacacggca atgaacagcg attacagctg ttaagtgatg cgcttggcga gcagtatgcg
    40081 cagcatggca actggcgctt cctgcgcaac aacgatcgct ttgtctttca gatcttgcgt
    40141 tcatttgaac acgataattc ggaagataaa cccggcccgg gtatgccgcc gcacggctgg
    40201 cgtactcaat tctgggtggt cgatcaaaac aacaaagtgc tggttggtcc gcgagcgccg
    40261 attccacctg acggtacacg gcgacccatt ctggtcaacg gtgcggaagt gggtgcggtg
    40321 attgcctccc ctgttgagcg actgacccgc aatactgata tcaatttcga taaacaacag
    40381 cggcaaacca gctggctgat tgtcgccctg gcaacgttac tcgcggcgct cgccaccttt
    40441 ctgctggcgc gtggtctgtt ggccccggtc aaacggctgg tggacggcac gcacaaactg
    40501 gcggcgggcg atttcacaac ccgcgtgacg cccaccagtg aagatgaact gggcaaactg
    40561 gcgcaggact tcaaccagct cgccagcacg ctggagaaaa accagcaaat gcggcgcgat
    40621 tttatggccg atatttctca cgaactgcgt acgccattag cggtgctgcg cggtgaactg
    40681 gaagccattc aggatggcgt gcgcaaattc acgccggaga cggtggcgtc tttacaggcg
    40741 gaggtcggta cactgaccaa actggttgac gatcttcatc agttgtcgat gtctgatgaa
    40801 ggcgctctcg cctatcaaaa agcaccggta gatttgatcc cactgctgga agtggcgggc
    40861 ggcgcatttc gcgaacgatt tgccagccgt ggcctgaaac tgcaattttc cctgccagac
    40921 agtattaccg tatttggcga tcgcgaccgt ttaatgcagt tattcaataa cttacttgaa
    40981 aacagcctgc gctacactga cagcggcggc agcctgaaaa tctctgccga gcagcacgac
    41041 aaaacggtgc gactgacctt tgccgacagc gcgccgggcg tcagtgacga tcagctacaa
    41101 aaattgtttg aacgttttta tcgcaccgaa ggttcccgca accgagccag cggcggttcc
    41161 gggctggggc tggcgatttg cctgaacatt gttgaagcac ataatggccg cattattgct
    41221 gcccattcgc cttttggcgg ggtaagcatt acagtagagt taccgctgga acgggattta
    41281 cagagagaag tatgaccgag ttaccaatcg acgaaaacac accgcgtatt ttgatcgtgg
    41341 aagatgaacc gaagctggga cagttgctca ttgattatct gcgtgctgcg agctatgcgc
    41401 cgacgcttat cagccacggc gatcaggtac tgccgtatgt gcgccagaca ccaccggatc
    41461 tgatcctgtt ggatctgatg ctccctggca ccgatggctt gacgctgtgc cgagaaattc
    41521 gtcgtttttc tgacattcct atcgtgatgg tgacggcaaa aatagaagag atcgaccgcc
    41581 tgctcggact ggagattggc gcagatgatt atatctgtaa gccgtacagc ccacgggaag
    41641 tggtagcgcg cgtcaaaacc attttgcgcc gttgcaaacc gcagcgtgag ttgcagcaac
    41701 aggatgctga aagcccgttg attatcgacg aaggtcgttt tcaggcttca tggcgcggta
    41761 aaatgcttga cctgacgcct gcggaatttc gtctgctgaa aacgctctct catgaaccag
    41821 gaaaagtgtt ctcccgcgag caattgctca atcatcttta tgacgactac cgcgtagtaa
    41881 ccgaccgcac catcgacagc cacattaaaa acctgcgccg caagctggaa tctctcgacg
    41941 ccgaacagtc ctttatccgt gccgtttacg gcgtcggtta ccgctgggaa gccgacgcct
    42001 gccgcatcgt ttaacatttt tacctgccgg agaaggcatt cacgccgcat ccggcaacat
    42061 aatcccacgc atggtcagca aactgacatt atctctccgc cctggcaggc accagaaaat
    42121 cgctcatact ttaatcggta aacagcacct ttagatgctg ttttcgatac gcaatttcaa
    42181 tcaaggagtc attatggctg gttggtttga actcagtaag agcagtgata atcaattccg
    42241 gtttgtgcta aaagcgggca atggtgagac tatcctcacc agcgagctgt atacctcaaa
    42301 agcctctgcg gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag aagaacgcta
    42361 tgagaaaaaa acggcaagca acggcaaatt ctatttcaat ctgaaagccg ccaatcatca
    42421 aattatcggc tccagccaaa tgtacgccac cgcgcaatct cgtgaaaccg gaattgcctc
    42481 cgttaaagcc aatggcacaa gccagacggt gaaagacaat acgtaattac gatgccgggc
    42541 gcgtgttgtt ggcgtccggc gctttaccaa ctccccccta cagcgctaca atgcccgccc
    42601 ttaaagtggg ggcactcccc taaccgcttc atcaggtgaa gcggatctga cctgtcatca
    42661 gaacgagaga attatgttta aaccggaact cctttccccg gcgggaacgc tgaaaaatat
    42721 gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc gttactccct
    42781 gcgtgtgcgc aacaacgaat tcaaccacga aaatcttcaa ctcggcatta acgaagccca
    42841 cgcgctgggg aaaaagtttt atgtcgtggt caacattgca ccgcacaacg ccaagctgaa
    42901 aacctttatc cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc tgattatgtc
    42961 cgatccaggg ctgattatgc tggtgcgtga gcacttccct gaaatgccga tccatctctc
    43021 ggtacaggct aacgccgtga actgggcgac ggtgaaattc tggcagcaaa tgggcctgac
    43081 ccgcgtgatc ctctctcgcg agctgtcgct ggaagagatt gaagagatcc gcaatcaggt
    43141 gccagatatg gaggtagaga tcttcgttca cggcgcgctg tgcatggcct actccggtcg
    43201 ctgcctgctc tctggctaca tcaacaagcg cgacccgaac cagggcacct gcaccaacgc
    43261 ctgccgttgg gagtacaacg tccaggaagg gaaagaagat gacgttggca acatcgtaca
    43321 caagtacgag ccgattccgg tgcaaaatgt tgagccgacg ctgggtatcg gcgcgccgac
    43381 cgacaaagtg tttatgatcg aagaagccca gcgtccgggc gagtatatga ccgcgtttga
    43441 agatgagcac ggcacttaca tcatgaactc gaaagatctg cgtgccatcg cccacgttga
    43501 acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa ggccgtacta aatctttcta
    43561 ctattgcgca cgcaccgcgc aggtttatcg taaagctatc gatgacgccg ctgcgggcaa
    43621 accgttcgat accagcctgc tggaaactct ggaaggtctg gcgcatcgtg gctataccga
    43681 aggtttcctg cgtcgtcata ctcacgacga ttatcagaac tacgaatacg gttattcagt
    43741 ttctgaccgc cagcagtttg ttggtgagtt taccggtgag cgcaagggcg acctcgcggc
    43801 ggtagcggtg aaaaataaat tctccgttgg cgacagcctt gagctgatga cgccgcaagg
    43861 gaacattaac tttacccttg agcagatgga aaacgccaaa ggcaaagcaa tgccggtcgc
    43921 accgggcgat ggttatactg tgtggatccc ggtcccgcag gatcttgagc tcaattacgc
    43981 gctgctgatg cgtaatttct ccggggaaac cacgcgtacc ccccacggta agtgattaat
    44041 ttcgattatt tttcccggat ggaaaattct tagaaaccga tcacatacag ctgcatttat
    44101 taaggttatc atccgtttcg ctgaaaaaca taacccataa aatgctagct gtaccaggaa
    44161 ccacctcctt agcctgtgta atctccctta cacgggctta ttttttacgc gtaagcccta
    44221 tccctggtca ccgtcttcca ttgaccacat cgatagaatc tcccttcata gcacgatgcc
    44281 tttcacgtaa cggcatcgtg ctcgcacagg ttccggctaa gcacaaccag aacgcgcatg
    44341 tttgacgctt accaaaaaat attctcactc tccacatttg aatgtcagac gagcgacgcc
    44401 atgtaatcct gcaccttctg tcttcaggtc aactatctgc atttttttgc cctgagtaac
    44461 acagaaatga gctgcatcat tttttactat attttctgca ccagaaattc tgcccctggc
    44521 taaagaagct tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa tattactttt
    44581 atatgcaaga ccaaatttac cgatacttgt ctcatcatta tgcacagcac acccagacat
    44641 aagaaaaaca ctaattaatg atatagcagc tatctttttc atcttacctt cccccattaa
    44701 ataccaacga cacgctttgg tgttaaaata taataatggc atgattatta taattgaata
    44761 ggattataat aaatgttctg tacaacattt
//
LOCUS       DAOTRK010000017        42580 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.017, whole genome shotgun sequence.
ACCESSION   DAOTRK010000017 DAOTRK010000000
VERSION     DAOTRK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.017"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(74..2578)
                     /gene="copA"
                     /locus_tag="QIE41_001028"
     CDS             complement(74..2578)
                     /gene="copA"
                     /locus_tag="QIE41_001028"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDS3529942.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMLGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHAVLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            2841..3773
                     /gene="glsA"
                     /locus_tag="QIE41_001029"
     CDS             2841..3773
                     /gene="glsA"
                     /locus_tag="QIE41_001029"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDS3529943.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            3776..5068
                     /locus_tag="QIE41_001030"
     CDS             3776..5068
                     /locus_tag="QIE41_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDS3529944.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHALLPILIVGTLVMLLVI
                     IGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            5405..6760
                     /locus_tag="QIE41_001031"
     CDS             5405..6760
                     /locus_tag="QIE41_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HDS3529945.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDIKLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHRTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            6869..27643
                     /locus_tag="QIE41_001032"
     CDS             6869..27643
                     /locus_tag="QIE41_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDS3529946.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVRDGNDLLIYMQDGTVIRCNGYFLQAANTSEQSELVFVDGQQLTHVTFADTAAGGL
                     APVELTAQTTAIESIAPYLDIVGQTTAFPWGWLAGAAVGGGALGALLASGGDDDSKTE
                     VVNNPPPAEPGNATPSFLVTDNQGDQRGILSANDTTDDTTPTFSGSGQAGATIQIKDS
                     NGDTIASTQVGSDGRWSVDLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASVQ
                     VATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQVP
                     TADAQALGEGNQAVLVSGKDATGNTVTGVQLLTVDTQPPTLAINTIAQDNIISAAEHN
                     VALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNAS
                     VSDRAGNSTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGDT
                     VTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDTAGNTGAASRAIT
                     LVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQA
                     DGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTAD
                     NIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGALA
                     DGNATVTASVNDIAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHTQAQIISGT
                     VTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLVDGSYPINVSVTDRAGN
                     TGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNGQ
                     NYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSNIGNSNTASHNVLVDSALPGV
                     TINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNFTWSVSV
                     PAADIQALGNGDLTVSASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIEH
                     GQALVVTGSSSGLAEGTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSTWPAGTVNIA
                     VSGESSAGNSVSITHPVTVDLTPAAIAINTIATDDVINAAEKGADLTLSGTTTNVEPG
                     QTVTVTFGGKNYTASVASDGSWTATLPAADLTALPEGSASAQASVSNINGNSASAVHN
                     YSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQA
                     TVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTINT
                     IAGDDIINAAEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAAD
                     VTALGSGPQTVMATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDIINATEKGADL
                     QISGTSNQPAGTTITVTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVTD
                     SAGNSNSASHNVQVNTALPGVTINPVASDDIINAAESGVAQTISGQVTGAAAGDTVTV
                     TLGGKTYTATVQGNLSWSVDVPAADIQAIGNGDLTVNASVTNGVGNTGSGSRDITIDA
                     NLPGLRVDTVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLADGT
                     WSLGVPAADVGNWPAGTVNITVSGTNTAGTTTTITHPVTVDLAAVAISINTVSGDDVI
                     NAAEKSADLTLSGSTSGVEAGQTVTVTFGGKTYTATVAGDGSWTTTVPAADLSALRDG
                     DATVQASVSTINGNTASATHAYSVDATAPTLAINTIATDDILNAAEAGNPLTISGSSN
                     AEAGQTVTVTLNGVTYTGTVQADGSWSVSVPTADLSNLTASPYTVSASVNDKAGNPAT
                     ATHGLAVDLTVPVLTINTVSGDDIINAAEHGQALVISGSSTGGEAGDVITVTLNSKTY
                     TTTLDASGNWSVGVPAADVTALGSGPQTITATITDIAGNSDDASRTVTVNLTAPTIGI
                     NTIASDDVINATEKSADLQITGTSNQPAGTTITVTLNGQNYTATTDSSGNWSATVPAS
                     AVSALGEASYTVTANVTDSAGNSNSASHNVLVNSALPAVTINAVATDDIINAAESGNA
                     QTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSVSVPAADIQAIGNGSLTVNASV
                     TNVVGNTGNGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSSGLTAGTAL
                     TVEINNVTYGATVLADGTWSLGIPAADVSNWPAGTVDITVSGTNSAGTTSTITHPVTV
                     DLAGVAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVESN
                     GSWTVNVPPADLAALPDGAGNVQASVSNINGNSAQADRAYSVDATAPLVTINTIASDD
                     ILNVSEAGAGITISGTTTAQVGQTLTVTLNNNTYQTTVQADGTWSVNVPATDLSGLTA
                     SSYTVTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGTS
                     TGAAAGDVVTVTLNGKNYTTTLDASGNWTVGIPAADVTALATGSQTITASLSDRAGNS
                     DSTTHNVTVDLSGPTLTISTVSDDDIINSTEKTQDLTISGGSSGLATGTTVTVMLNGL
                     AYSATTDSSGNWSVTVPASAVGALGEAVYQISASATDSAGNNGSTTHTVNVESLLPGV
                     IINTVAGDDIINAAEIAVAQTISGQVTGTAVAGNTVIVTIGGNQYTATVQPDLSWSVS
                     VPANVLQALGNGELTISASVTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIE
                     HTQALVITGSGSGLAAGAALTVVINSVTYGATVLADGTWSVGVPAADVTNWPAGTVNI
                     AVSGTNTAGTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSDVEA
                     GQTITVIFGGKSYTTTVAAGGTWGLTIPAADLATLPDGAANVQASVSNVAGNSAQATH
                     AYSVDATAPSVTINTIASDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNV
                     QADGSWSVSVPTGDLANLTASPYTVSAAVSDKAGNPASATHNLTVDLAAPVVTINTVA
                     GDDIINATEHAQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVIS
                     ALAQGDVTITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINATEKGADLAI
                     SGSSNQPAGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDSD
                     GNSGSASHNVQVNTALPGVTINVVATDDIINAAEAGVDQTISGQVTGATAGDTVTVTL
                     GGATYTATVQANLSWSVDVPAAALQALGNGELTISASVTNSVGNTGNGTREITIDANL
                     PGLRVDTVAGDDVVNIIEHGQALVITGSSSGLATDSNVTLTINGQTYVAAVLADGSWS
                     VGVPAADVSAWPAGTVTITASGSTTAGNPVSVTHPVTVDLTAVAVSINAITADDVINA
                     AEKGAALTLSGSTSGVEAGQTVTVTFGGKTYTASVAANGSWSTSVPAADMAALRNGDA
                     SAQASVSNVNGNNATTTHAYSVDASVPTVTINTIAGDDILNAAEAGAALTITGSSTAE
                     AGQTVTVTLNGANYTGTVQTDGSWSISVPPADLSALTASNYTVSAAVSDKAGNPASVN
                     HNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTT
                     VLDASGNWSVGVPASVVSALANGTVTINASVTDAAGNSGSATHQVTVNTGLPSITFNA
                     ISGDNVLNADEKGQPLTISGSSTGLATGAQVTVTLNGHNYSATTDAAGNWTLTVPVSD
                     LAALGQANYIVSASATSAAGNTASSQANLLVDSGLPGVTINTVAGDDIINAAEAGAAQ
                     TISGVVTRAAAGDTVTVTLGGNTYTAQVQADLSWSVSVPAADLQALGNGDLTITASVT
                     NANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGVPLT
                     ITINGTAYSATVQADGSWSVGIPAANVSAWPAGALTVEVDGQSSAGNPVGVSHPFTVD
                     LTAVAISISTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANG
                     SWSVNVPAADLAILPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDI
                     LNATEAGSPLIISGTSTAETGQTVTVTLNGATYSGNVQADGSWSVSVPPSALGALSAS
                     NYTVSATVNDKAGNPGSASHNLAVDTTAPVLTINTVVGDDIINDAEHAQALVISGTST
                     GGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAGNSD
                     DASRTVTVSLTAPVISINTIAGDDVINATEKGSDLALSGISDQPAGTAITVTLNGQNY
                     SATTDSSGNWSVTVPASAVSALGEASYSVTASVTNAQGNSSTASHNVQVNTALPGVTI
                     NPVTTDDIINAAEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPA
                     ADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHSQ
                     AQVITGSSSGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVS
                     GANSAGTQTSITHPLTVDLTTVAVSINSITSDDVINAAEKGAALTLSGSTSGVEAGQT
                     VTITFGGKTYTTTVAANGSWSTTVPAVDMATLRDGDASAQVRVTNVNGNSATATHEYS
                     VDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAETGQTLTVTLNGTNYQTTVQAD
                     GSWSLTLPASDLTALANNGYTLTASVSDLAGNPGSASKGVTVDTTAPVISFNTVAGDD
                     VINNVEHTQAQIISGTATGAVAGDRLVVTIAGQQYVSSTDASGNWSVGVPASVISGLA
                     DGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLSGT
                     GTNFAAGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAG
                     NSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGG
                     KTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQTTHALTVDTVAPT
                     VTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWTATVGSGGSWSVFI
                     PAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPSVTINTFAGDDVVNAAEH
                     GSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSVDVTALADGNAYVIN
                     ASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSSSDFITSVSPVVVNGSLT
                     AALASNETAQISIDGGVTWTTLTVTGTIWRYNDSRTLTDGSYLYQVRVIDAAGNVGAT
                     DSQNVVIDTIAPDPAVKTIAINAITTDTGLITNDFVTSDTTLAVSGTLGAALSSGEFA
                     QISIDGGTTWQNLSVSGLTWTYLDGRTLSDGNYNYQVRVIDTAGNIGATASQIVTVDT
                     TAPLASKTIAIAGISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVTW
                     TTLTVIGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSIT
                     ITGISDDTGASSSDFITSDTSLTVRGVLGAALGANEFAQISTDNGATWVNVTLAADGL
                     NWSYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSAQIDTVNPAQVLTIASISTDTGS
                     SATDFITSDTTLTLTGSLGAGLASGEVAQISLDGGATWITLTTNGTQWTYTDSRTLTD
                     GSYVYQVRVLDLAGNTGSVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQATD
                     DTTPLLNGVLSGPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSARV
                     VDLAGNITASSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVVI
                     NGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALAASATAYNVTAQVKSSAGNGNNANIS
                     NGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKTA
                     LTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTVG
                     TLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATVG
                     GAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTNT
                     FLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAVG
                     ATATTYASQFSVAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQSGTT
                     SGVAAMDYDWDGAVDVLVTKQSGSVYLIRNTNTVSYGTSLHLRITDPNGINVYYGNTV
                     KLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNIDQ
                     TVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGWV
                     YSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFND
                     TLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTWE
                     GTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQI
                     DRDGTGGTFAATNVVTLTGVHTDLATLLANHQLMVV"
     gene            27647..29809
                     /locus_tag="QIE41_001033"
     CDS             27647..29809
                     /locus_tag="QIE41_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDS3529947.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSTDSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVTDIEDNIVELALLRPLSAIPDARVDDYIRPYQANWFWNLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAIFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTTRRVVLQEKNKGSAA"
     gene            29806..30981
                     /locus_tag="QIE41_001034"
     CDS             29806..30981
                     /locus_tag="QIE41_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDS3529948.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            30978..31385
                     /gene="cueR"
                     /locus_tag="QIE41_001035"
     CDS             30978..31385
                     /gene="cueR"
                     /locus_tag="QIE41_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDS3529949.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(31589..32011)
                     /locus_tag="QIE41_001036"
     CDS             complement(31589..32011)
                     /locus_tag="QIE41_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529950.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(32065..33081)
                     /locus_tag="QIE41_001037"
     CDS             complement(32065..33081)
                     /locus_tag="QIE41_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HDS3529951.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLTNTQKRVADNSQQINTLNNHFDSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            33448..33810
                     /locus_tag="QIE41_001038"
     CDS             33448..33810
                     /locus_tag="QIE41_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529952.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKNMNKGSDE"
     gene            complement(33995..34453)
                     /locus_tag="QIE41_001039"
     CDS             complement(33995..34453)
                     /locus_tag="QIE41_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDS3529953.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(34450..35367)
                     /locus_tag="QIE41_001040"
     CDS             complement(34450..35367)
                     /locus_tag="QIE41_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDS3529954.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            35513..36190
                     /gene="fetA"
                     /locus_tag="QIE41_001041"
     CDS             35513..36190
                     /gene="fetA"
                     /locus_tag="QIE41_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDS3529955.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            36177..36956
                     /gene="fetB"
                     /locus_tag="QIE41_001042"
     CDS             36177..36956
                     /gene="fetB"
                     /locus_tag="QIE41_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDS3529956.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(37019..37873)
                     /gene="cnoX"
                     /locus_tag="QIE41_001043"
     CDS             complement(37019..37873)
                     /gene="cnoX"
                     /locus_tag="QIE41_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDS3529957.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37934..38743)
                     /gene="ybbO"
                     /locus_tag="QIE41_001044"
     CDS             complement(37934..38743)
                     /gene="ybbO"
                     /locus_tag="QIE41_001044"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDS3529958.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGQESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(38733..39356)
                     /gene="tesA"
                     /locus_tag="QIE41_001045"
     CDS             complement(38733..39356)
                     /gene="tesA"
                     /locus_tag="QIE41_001045"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDS3529959.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            39327..40013
                     /locus_tag="QIE41_001046"
     CDS             39327..40013
                     /locus_tag="QIE41_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3529960.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            40010..42424
                     /locus_tag="QIE41_001047"
     CDS             40010..42424
                     /locus_tag="QIE41_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3529961.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLSLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIIAGNW
                     PPKADEVSMEEGLAKRLNVGLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLHTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         9750 a        11944 c        12123 g         8763 t
ORIGIN      
        1 actttatatg gcattactca taaaaggagc gcaacaagaa ctggatctca caaacgcatt
       61 aagtaaatac caatcattcc ttcggtttaa accgcagcaa ccggttggcg ttactcacta
      121 cggtaatcga cgagagcgcc attgccgctc cggcgaccac cgggttaagc agtgttccag
      181 tgaacggcca caaaataccg gcggcgaccg gaataccgat actgttgtag ataaacgcgc
      241 cgagcaggtt ctgcttcata ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa
      301 cacccatcag gctatggcgc atcagggtaa tcgccgcggt ttcaatggca acatcactgc
      361 cgccacccat cgcaataccg acatccgcct gagccagcgc tggcgcgtcg ttaatgccgt
      421 cgcccaccat tgccacctga cgtccttcac tttgcagacg tttgatcgct tcggctttac
      481 cgtccggtag caccccggct atcacctcat caatccctgc ttctttggcg atcgcattgg
      541 cggtggttgg gttatccccg gttagcatca ccagacgata tcccgcttta tgcaggcgtt
      601 gcagcgccgc cacgctatca ctacgcaatg gatcgcgtac agccagcagg gctaccgctt
      661 tcccgtcaac cgccagcagc acaggcgttg ccccttgtga tgcctgagct gaaatatctg
      721 cctcaatcgc tttagtatca acttgctggt catttaacag cgcctgattg cccagcaata
      781 ccgcatgacc ttcagcttca ccgctcacac ccagcccgcg caatgtgcgg aaaccgttga
      841 cctgcggtag ctgcatatca cctgctttat cgaggatcgc ccgtgccagc gggtggctgg
      901 aaccttgctc cagcgccgcc gccagacgca atgcctgcgc ttcatcaaca tcagcaaatg
      961 tttttactgc gacaacctgc ggcttccctt cggtcagcgt cccggtttta tcgaacacca
     1021 cggtgtcgag cgtactggcg cgttgcagcg cgtcggcgtc ccgcaccagc acaccaaact
     1081 cagccgcccg cccgacgccc gaaataatcg acatcggcgt cgccagtccc agcgcacacg
     1141 gacaggcgat aatcagcacc gtggtggcaa tcaccagggt atagacaatc tgcggtgccg
     1201 gaccaaagaa ataccagatt gccgcactga tcagcgcaat gactaccact accggcacaa
     1261 acacggcgga gattttatcc gccagttgac cgatttctgg cttgctgctc tgggcctgtc
     1321 gcaccatgcg aatgatccgt gacagcgtag tatggctgcc gaccgcactg gcacgaaaca
     1381 gcacactgcc gtcctgtacc actgtcccgg catggacgct atcgccttcg cctttttgct
     1441 gcgggatagg ttcgcccgtc agcatcgctt catccagcca cgcttcgcct tgggtaatct
     1501 caccatctac cggcacacgg tcgccggtcg tcaggcgcag taacatacct ggctgaacat
     1561 ctgccagcgg cacgtttttt tcaccttcgt cggtgaccag gcgcgccgtc ggtggggtta
     1621 aatcgagtaa tttctccagc gccttagaag aacgctgacg cgcgcgcgct tccagcatat
     1681 ggccgagatt gatcagaccg ataatcatcg cgctggcttc gtaataaaga tgtcgcgctt
     1741 ccatcgggaa ccactgcggc cacaggttga cgctcatcga atagagccac gccacgccag
     1801 tacctagcgc caccagcgta tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac
     1861 tacggtaaaa atggccgccg gcgaaaacca tcactgccag ggttatcagg ccgataacta
     1921 accacaagct gcggttgtca gcggtgacca tcatgttatc gccgagcatc ccccagacca
     1981 tcaccgggat acccaccgcc agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag
     2041 cgactgcggt ttcctgctgg cgctcgcggc gtttagcgtc atcttcaatc gcttccgcgc
     2101 cgtagcccgc tttttccacc gcctgcacta aatcttgtgg ggaggcactg cccatcacca
     2161 gcgcagtacg ctccgccagg tttacccgtg cctgagtgac gcccggtacg ctttgcaacg
     2221 cattttgtac gcgggtgaca cagctggcgc agctcatgcc gctcagcagc aactgctggc
     2281 tgtcatcgtc atcggcggtc gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg
     2341 acgggattga tgactccgcc agcggtttag cctttgggtg gcttacagat gcgtcataac
     2401 ccgcttgttt gatggtttcg atcagctgtt ctgcactggc agtcccggta acgtgcgctt
     2461 cagtgacaga cacatccgcc tgctcaacat ccgggcgctg ttcgagactt tctttcacgc
     2521 gtttaacgca gtgaccgcag gacaggccgt ccagggtcag gtcgatagtt tgtgacataa
     2581 aacactcctt ttaagacagt tttgactggc tgtgataaag gttaaacctt ccagcaaggg
     2641 gaaggtcaag aaattaataa accaggcggg taaaagtccg taaagattaa aaaatcggct
     2701 cgatttgcat caggattaga catttatctc tttgttttcc tgtagttaag ttgcgggtgc
     2761 taagttaaat caggatgcct gaaaatcggc accggggtga ggaattacct cccgcatcta
     2821 taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaggca gtggatcagg
     2881 cttacaccca atttcactca cttaacggcg gacaaaatgc cgactacatt ccctttctgg
     2941 cgaatgtacc tggtcaactg gcagcagtgg ctattgtgac ctgcgatggc aacatctatc
     3001 gcgcgggtga tagtgattac cgttttgcgc tggaatccat ctcgaaagtc tgtacgttag
     3061 cccttgcgtt agaagatgtc ggcccgcagg cggtacagga caaagttggc gctgacccga
     3121 ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa ccgctgtcgc
     3181 cactggtaaa tgctggcgct attgccacca ccagcctgat taacgctgaa aatgccgaac
     3241 aacgctggca gcgaatttta catatccaac aacaactggc tggtgagcag gtagcgctct
     3301 ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca taaccgggcc attgcctggc
     3361 tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac gtgtacaccc
     3421 gtcagtgctc cacgctcatc aatactgttg aactggcaac gcttggcgcg acgctggcgg
     3481 cgggtggtgt gaatccgctg actcataagc gcgttcttca ggccgacaac gtgccgtaca
     3541 ttctggctga aatgatgatg gaagggctgt atggtcgctc cggtgactgg gcgtatcgcg
     3601 ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc cctggcgtga
     3661 tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caacagtgtt cgcggtcaaa
     3721 aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc tgatcatgat
     3781 gaacacggaa ggtaataacg gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg
     3841 tattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg cgttgctaat
     3901 ggaagcctcg acctgggtcg cctttgcatt tggcggtatt gtggcgatgt tttccggtta
     3961 tgcctatgcg cgcctggggg cgagctatcc cagcaatggc ggtattatcg acttctttcg
     4021 tcgcggatta ggcaacggcg tcttttcgct ggcgctctcg ttactgtacc tgttgacgct
     4081 ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc agtttttgca
     4141 tgaaggcagc caggaggagc accttatatt gctctacgcg ttggggatca ttgcggtgat
     4201 gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg
     4261 cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc tgcaaccggc
     4321 gcatatttct gtctctgcgc cccccagctc cggtgcgttc ttctcctgta tcgggataac
     4381 cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc
     4441 gcaggtcatt atgccacggg cgtttctggt ggcgattggc gttaccacgt tgctttatat
     4501 ctcgctggcg ctggttttgc ttagcgatgt atcggcttta gagttagaaa aatatgccga
     4561 taccgccgta gcgcaggcag cttctccgct gctcgggcat gtgggttatg tgatcgtcgt
     4621 catcggcgct ttactggcga cggcttcagc catcaacgcg aacctgttcg ccgtgtttaa
     4681 catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata aatccctgtg
     4741 gcggcagagt acctggggca acatcattgt cgtggtgctg attatgctga tgacggcggc
     4801 gctgaattta ggctcactcg ccagcgttgc cagcgccacc tttttgatct gctatctggc
     4861 ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gccttgttgc cgattcttat
     4921 cgttggtacg ttggtgatgt tgttggtgat cattggcttt atctacagcc tgtggtccca
     4981 gggcagccgt gcgttgatat ggattattgg tgcaatctta ctcagcctta ttgtggcaat
     5041 ggtcatgaag cgcaataaaa ccgtataaca cctctctgtg cgcagtacag cctgtactgc
     5101 gcctttgtct ttaattcaaa ttacctttct tattccttta ataaataagt cacaaaaaat
     5161 gatatatacg cgataaatct gtgatgctaa tcacttattg ccgtggatta ttcttaatgt
     5221 gtatgatggt aatctaaaag tcgtagtgta ttctaaataa ataacgtata agctttttag
     5281 ctgcatttct ttgccaatat caatgatcga caaaagagat gcacaagtta ttacccttct
     5341 taataaaatg agactagtta taaaaaccag ttaaaataat gacctggaag gaagtgactt
     5401 atctatggcg cgattccagt ttaaaagccg taaaaataat gggtttattt ttttaataag
     5461 ttttatggtg atgggagaag ctacaattgc tgcaccgctg ccacaatggg cgaacgcccc
     5521 agcggtaacg ccagttgccc aattatcttt acaggaaagt atattacgcg cctttgcgcg
     5581 aaatcccgac gtcactcaac aggcggcgca gataggtatt ggcgaagcgc aaattgatga
     5641 agccaaaagt gcctggtatc cgcacgttgg attaaccggc aatgcgggac cgtcccgaca
     5701 aacggactcc agtggcaggc ttgataacaa cgtttcgtat ggcataaccc tgacacagct
     5761 ggtgtatgac tttggtaaaa ctaacaacga tatcaaactg caaactgccg cccgtgacag
     5821 ctaccgcttt aaattgatgg caaccttaac cgatgttgca gagaaaacgg cgactgccta
     5881 tatggaagtc agtcgttatc aggctttgtg cgatgcggct caacgcaata ttcactcgct
     5941 ggaaaacgtc tacaacatgg cagcattgcg tgctaacgct ggcctgaact cgtcgtcgga
     6001 tgaattacag gcccagacgc gcattgccgg aatgcgctca acgctggagc aatatcaggc
     6061 gcagatggca agcgccaaag cgcaactggc ggtgctcact ggcgtacagc cggaggcgat
     6121 agccgcgcca cctgctgaat tagccgagca gccggtatcg ctgaaaaata ttgattacca
     6181 gtccatcccg ctggtgctgg cggcagaaaa cttacgccag tcagcacagt acggcgtgga
     6241 aaaaacgaaa gcgcaatact ggccaacgct gagtattcag gggggcaaaa cgcgctacca
     6301 gaccagcgac cgctcgtatt gggatgatca gctacaactg aacgttaacg cgccgctgta
     6361 tcagggcggc gcggtttctg cccaggtgca acaggctgaa gggcaacaaa aaatctctgc
     6421 ctcgcaggtc gaacaggcaa aactggatgt gctgcaacga gcgtctgtgg catatgccaa
     6481 ctggacaggc gcacggggtc gtgaagaggc tgggttagcg caatctgaaa gtgcgcacag
     6541 aacgcgagat gtctaccaaa atgaatataa attgggtaaa cgcagcctga atgatctatt
     6601 aaccgtcgag caagatgtct ttcaggcgca atcggctgaa ataaatgcca attatgatgg
     6661 ctgggtcgcc gcagtgaatt atgccgctgc ggttaataac cttattccat tagcgggaat
     6721 taaacagggc ttatacaacg acttacccga cttgaaataa tcctgccatt attaatatca
     6781 ggtatgcatc gttgttgagt gattaccttt gtcttattta gataaatgga ctcgctaagg
     6841 atgtgcgcca tttatcagga atgccattat gagcctgatt attgatgtta tttcgcgaaa
     6901 aacatccgtc aaacaaacgc tgatcaatcc tggcgacgtt acggttgtca tttatgagcc
     6961 ctctgtggtg caggttcatg ctcaggcatc tgccgttgcg cgttatgttc gtgacggaaa
     7021 tgacttgttg atctatatgc aggacggcac ggtgatccgc tgcaacggtt atttcctgca
     7081 agcggcaaat accagcgaac aatccgaact ggtgtttgtc gatggtcaac aactgaccca
     7141 cgtaacattt gccgataccg cggcaggtgg attagcgccc gtggagctga ccgctcagac
     7201 caccgcaatt gaaagcattg cgccgtacct tgatatcgtc ggccagacga cggctttccc
     7261 gtggggatgg ctggcggggg cggcggtagg tggcggcgcg cttggcgctt tactggcaag
     7321 cggtggcgat gatgactcaa aaactgaagt ggttaacaac ccaccgccag ccgaacccgg
     7381 caacgccacg ccatcgtttt tggtaaccga taatcagggc gatcaacgcg gtattctttc
     7441 cgccaacgat accaccgatg acactacgcc aacctttagc ggcagcgggc aggcgggggc
     7501 gaccatccag attaaagaca gcaatggtga caccatcgcc agtactcagg tcggtagcga
     7561 tggacgctgg agcgtcgatt tgcccacgca aagcgcgggc gaacatacct ggtcagtggt
     7621 gcaaatcgtc ggcagtacca tcactgacgc cggttcgata acgttaacca tcgacaatag
     7681 tcaggccagc gtgcaggttg ccaccaccgc aggcgataac attattaacg ccagcgaaca
     7741 ggccgccggg tttacgcttt ctggcaccag tagtcatctg gcacagggaa cagaactcac
     7801 cgttacgcta aacggcaaaa cctacaccac cagcgtaggc gctaacggtg cctggagcgt
     7861 gcaggtaccg accgccgatg cgcaggcgtt aggcgaaggg aatcaggcag tgctggtcag
     7921 cgggaaagac gccacaggca ataccgtcac cggcgtgcaa ctgctaacgg tcgataccca
     7981 accgccaacg cttgccatca acaccatcgc tcaggacaac atcatcagtg ctgcggaaca
     8041 taacgtcgcg ctggtactga gcggcacgtc gaatgcagaa gcggggcaaa ccgtaacact
     8101 gaccgtcaac gggaaaagcc atacagcaac cgtcggtagc gacggaacct ggcaagtgac
     8161 gctgcctgcc acggaagtcc aggcgctggc ggagggtaat tacgctgtca atgccagtgt
     8221 cagcgatcgg gcagggaaca gcaccagcca cagcgcgaat ttcacggtag atacctcagc
     8281 acccgtggtc agtgttaata ccgtagcggg cgacgatatt cttaataatg ctgagcaggc
     8341 cgtcgcgcag atcatctccg gacaagtcag cggtgcttct cctggcgata cggtaacggt
     8401 gaaattgggc actcatgtcc tgacgggcat tgtgctggca gatggcagct ggaatgtggc
     8461 gctggaccca gcggtaaccc gcacgctgga tcgcggagcc aatacgattt tcgtcaccgt
     8521 gacagatact gcaggaaata ccggcgcggc gtctcgagca atcacgctgg tcggtgtttc
     8581 tccgttaatc accattaaca ccgtctccgg cgatgacatt atcagtggcg cagaaaaagg
     8641 tgcgccactg acccttaccg gtagcactca acaggccgag acaggacaaa ccgtcacagt
     8701 aaccctggct ggacagagtt ttaccactac cgtgcaggct gatggctcct ggagtctgac
     8761 ggtacctgcc gccgcgatgg gaaatctgcc tgacggcgcg gtggcgatta ccgcttctgt
     8821 gacggatctc agcggcaata ccggcaacac ctcccgcacc attaccgtcg acagccaggc
     8881 cccggcgttg agcatcgatc cactgaccgc tgataacatc attaacgccg ccgaaagcgg
     8941 gcaggatttg cccatcaccg gcaccaccga cgctcagcca gggcagacgg tgaccgttac
     9001 gttgaatggg cagacgtacc agggcgtcgt gcagccagac ggcacctgga gcgtgaccgt
     9061 tcccgccgcc aacgttggcg cactggctga cggcaacgcc accgtcaccg ccagcgtgaa
     9121 cgatatcgcc ggtaacccga ccagcgtctc acgcgtggcg ctggtcgatg ccacgccgcc
     9181 ggtggtaacc attaatccgg tggcgaccga taacgtcatc aacacgccgg aacacaccca
     9241 ggcgcaaatc atcagcggca cggttactgg cgctcaggcg ggcgatatcg tcaccgtgac
     9301 gctgaataat gtggattaca caacggtggt ggatggttcc ggcaactgga gcctgggcgt
     9361 tccggcttcc gttgtcagcg gactggtgga tggcagttat ccgatcaacg tgtcagtgac
     9421 tgaccgcgcc ggaaacacgg gcagccagtc attgaccgtc acggtcaata ctgccgcgcc
     9481 ccttatcggc attaacagca ttgcgggcga tgatgtgatt aacgccagcg aaaaaggggc
     9541 cgatctccag atcaccggca ccagcgatca gcctgttaat accgccatca ccgtgacgct
     9601 gaacgggcaa aattacacca ccacgaccga cgcctccggc aactggagcg tcaccgttcc
     9661 ggcatcggcg gtaaccgcac tgggccaggc caactacacg gtaacggcgg cggtgacgag
     9721 taatatcggc aacagcaaca ccgcaagcca taacgtgctg gtcgacagcg cgctgcccgg
     9781 cgtgaccatt aacccggtgg caaccgacga tattattaac gccgccgaag cgggcgttgc
     9841 gcaaaccatt agcgggcagg ttactggcgc ggaagatggc gacacggtaa ctattacgtt
     9901 gggcggtaat acttatacgg cgacggtggg cagcaatttc acctggagcg tgagcgttcc
     9961 ggcggcagat attcaggcgc tgggtaatgg cgatttaacg gtgagtgcct cagtcaccaa
    10021 tcaaaacggc aacaccggca gcggcacgcg ggatatcacc atcgacgcca atctgcccgg
    10081 cctgcgggtc gatacggtgg cgggcgatga tgtggtcaat atcatcgagc acgggcaggc
    10141 gctggtggtc accggcagca gttcggggct ggcagaaggc acgccgctta ccgtcacgat
    10201 taataatgtg gaatacacca ccgcggtgca ggccgatggt agctggagcg tgggcgtcac
    10261 tgcggcgcag gttagcacct ggcctgcggg gacggttaat attgctgtat ccggggaaag
    10321 tagcgccgga aactcggtga gcattacgca tccggtgacg gtcgatctca ctccggcggc
    10381 gatcgccatc aacaccatcg ccacggatga cgtgattaac gccgcagaaa aaggcgctga
    10441 tttaaccctt tccggcacca ccactaacgt agaacccggt caaaccgtca ccgtcacctt
    10501 tggcgggaaa aattacaccg ccagcgtagc aagcgacggt agctggactg ccaccttacc
    10561 tgccgccgat ctgacagcat taccagaggg cagcgcctcc gcgcaggcca gcgtcagcaa
    10621 tattaatggc aatagcgcct cggcggtgca taactacagc gtcgacagca gtgccccgac
    10681 catcattatc aataccgtcg ccagcgacaa tatcgtcaac gccagcgaag ccgatgcggg
    10741 tgtgacggtg agcggcagca ccaccgctga agcggggcag attgttacgg taacgctcaa
    10801 tagcccgacc gtgcagacct atcaggcaac ggtgcaggcg gacggcagct ggagcatcaa
    10861 tattccggcg gcagatcttg aggcattgac cgatggcagc cacaccctga ccgccacggt
    10921 caatgacaaa gcgggcaacc cggcgagcac cacgcataat ctggcggtgg atctcaccgt
    10981 tccggtactt accatcaaca ccattgcggg cgacgatatt attaacgccg ccgaacacgg
    11041 gcaggcgctg gtgatttccg gttccagcac tggcggagaa gcgggggatg tcgtcaccgt
    11101 cacgctaaac agtaaaacct acaccacgac gctggatgcc tccggcaact ggagcgtcgg
    11161 cgttccggcg gcggatgtca cggcgctggg tagcggcccg caaaccgtga tggcgaccgt
    11221 taccgatgcg gcaggtaata gcgacagcga aacgcacacg gtcaccgtta accttaccgc
    11281 gccaaccatt ggcattaaca ccatcgctac cgatgacata attaacgcca ccgaaaaagg
    11341 cgcggatctg caaatttccg gcaccagcaa tcagccagcg ggcaccacta ttacggtgac
    11401 actgaacggg caaaattaca ccgccacgac cgatgcttcc ggcaactgga gcaccacggt
    11461 gccagcctcc gctgtcggtg cgctgggcga agccagttac acggtgacgg cgaacgtcac
    11521 cgatagcgcg ggcaacagca attccgccag ccataacgtg caggtcaata ccgcgctgcc
    11581 tggcgtcacc attaatccgg tggcgagcga cgatattatt aatgccgccg aatcgggcgt
    11641 ggcgcaaact atcagtgggc aggtgacggg cgcggcggcg ggcgataccg tcaccgtgac
    11701 acttggcggg aaaacttaca ccgctaccgt gcaggggaat ttaagctgga gcgtggacgt
    11761 tccggcggcg gatattcagg ccatcggtaa tggcgatctc acggttaacg cctcagtgac
    11821 caacggcgtg ggtaataccg gcagcggttc gcgcgatatt actatcgacg ccaatttgcc
    11881 aggtctgcgc gtcgataccg tggcgggcga tgatgtggtt aacagcatcg agcacggtca
    11941 ggcactggtg ataaccggta gcagcagcgg gctggcggcg ggcgcggcgc tgacggtggt
    12001 gatcaataac gtcacctatg gcgcgacagt attagccgat ggcacatgga gccttggcgt
    12061 tccggcggca gatgtgggca actggcctgc ggggacggtg aatatcacgg taagcggcac
    12121 taacaccgcc ggaacaacta cgacaatcac ccatccagtc accgtcgatc tggcagcggt
    12181 ggcgatctcc attaacaccg tttccggcga cgatgtgatt aatgccgctg aaaaaagtgc
    12241 agatttaacc ctttcaggca gcacctccgg cgtggaagcg gggcaaacgg tcaccgttac
    12301 ctttggcggg aaaacctaca ccgctaccgt agcgggcgat ggtagctgga caaccaccgt
    12361 acctgccgcc gatctcagcg cgttacgcga cggcgacgcc acggtacagg ccagcgtcag
    12421 cactattaac ggcaacacgg cttcggcaac ccacgcctac agcgtcgatg ccacggcccc
    12481 gacgcttgcc atcaatacca tcgccaccga cgatattctg aacgccgccg aggcgggcaa
    12541 tccgctaacc atcagcggca gcagtaacgc cgaagcgggg cagacggtaa ccgtcacgct
    12601 taatggcgtg acttacaccg gcaccgtcca ggctgacgga agctggagcg tcagcgtacc
    12661 gacggcggat ctcagcaatc tgaccgccag cccgtacacg gtcagcgcct cggtaaacga
    12721 taaagcgggt aacccggcga ccgccactca cggcctggcg gtggatctca ctgttccagt
    12781 gctgaccatc aacaccgtct ccggcgatga cattattaac gccgccgaac acggacaggc
    12841 gctggtgatc tccggctcca gcactggcgg ggaagcgggc gatgtcatca ccgtcacgtt
    12901 aaacagtaaa acctatacca ccacgctgga cgcttccggc aactggagcg tcggcgttcc
    12961 ggcggcagat gtcactgcgc tgggcagcgg cccgcaaacc attactgcca ccattacgga
    13021 tatcgcgggt aatagcgatg acgccagccg cacggtcacc gtgaatctga ccgcgccaac
    13081 cattggtatc aacactatcg ccagcgatga cgtgattaac gccacggaaa aaagcgcgga
    13141 tctgcaaatc accggcacca gtaatcaacc cgcaggcacc acaataacgg tgacgctgaa
    13201 cgggcaaaat tacaccgcga cgaccgattc ctccggcaac tggagcgcca cggtgccagc
    13261 ctcggcggtt agcgcgctgg gggaagccag ttacacggta accgcgaacg tcaccgatag
    13321 tgcaggcaac agcaattccg ccagccataa cgtgctggtc aacagcgcgc tgcctgccgt
    13381 caccattaac gcggtggcga ccgacgatat tattaacgct gctgaatccg gaaacgcgca
    13441 aaccatcagc ggccaggtca cgggcgcggc ggcgggcgat acggtcaccg ttacgctggg
    13501 cggcaacacc tacaccgcca cggtgcaggc taatttaagc tggagtgtca gcgttccggc
    13561 ggcagatatt caggccatcg gcaacggctc tctcaccgta aacgcctcgg tgaccaacgt
    13621 cgtcggcaac accggcaacg gttcgcgaga catcactatc gatgccaatc tgcctggcct
    13681 gcgggtcgat accgtggcgg gcgatgatgt tattaacagc attgagcaca atcaggcgct
    13741 ggtgatcacc ggcagcagca gcggattaac ggcgggaacg gcgttaacgg tcgagattaa
    13801 caacgttact tatggcgcga cggtattagc cgacggcacg tggagcctcg gtattccggc
    13861 ggcagacgtc agcaactggc ctgcgggtac ggtggatatt acggtaagcg gcaccaacag
    13921 cgccggaaca acctccacca ttacccatcc ggttaccgtc gatctggctg gggtcgccat
    13981 caccattaac actctctctg gtgatgatgt gatcaacgcc gtcgaaaaag gcgaaacgct
    14041 ggtcgtaagc ggcagcacca gcggtgtgga agctgggcaa acggtgactg tcacctttgg
    14101 tggcaaaaat tacaccacca ctgtggaatc taacggtagc tggacggtga atgtgccgcc
    14161 tgccgatctc gctgcgctac cggacggcgc gggcaacgtg caggcgagcg tcagtaatat
    14221 taacggcaac agcgcccagg cggatcgcgc gtatagcgtt gatgccaccg cgccgcttgt
    14281 gaccatcaac accatcgcca gcgacgatat ccttaacgtg agcgaagcgg gcgcagggat
    14341 caccatcagc ggcaccacca cggcacaggt cgggcagacg ctgaccgtca cgctgaataa
    14401 caacacgtac cagaccaccg ttcaggcgga tggcacctgg agcgtgaatg ttccggcaac
    14461 ggatttaagc ggattaaccg ccagcagtta taccgtgact gcgacggtga gcgacaaagc
    14521 gggcaacccg gcaagcgccg atcatgcgct ggcggtggac gttaccgcgc cggatctcac
    14581 cattaatacc gtcgcgggcg atgacattat caacgccatc gaacatggtc aggcgctggt
    14641 ggtcagcggc accagcacgg gcgcggcggc gggtgatgtg gtaaccgtca cgctcaacgg
    14701 caaaaactac accaccacgc tggacgcctc cggtaactgg actgtgggca ttccggcggc
    14761 ggatgtcacg gcgctggcga ccggtagcca gacgattacc gccagcctga gcgatcgcgc
    14821 gggcaacagc gacagcacta cccataatgt gaccgttgat ctcagcggcc cgacgctgac
    14881 catcagtacc gtctccgacg atgacattat taacagtacg gagaaaaccc aggatctgac
    14941 catcagcggc ggctccagcg gtctggcgac tggcaccacg gtcaccgtga tgttgaacgg
    15001 cctggcgtac agcgccacta ccgatagttc cggtaactgg agcgttaccg tgcctgcttc
    15061 ggcggtcggc gcactgggcg aggcggtata ccagattagc gccagtgcca ccgacagcgc
    15121 gggcaacaac ggcagcacca cccatacggt gaatgtcgaa tcgctcctgc ctggggtgat
    15181 cattaatacg gtggcgggcg atgacattat caatgctgct gaaatcgctg tggcgcagac
    15241 catcagcggt caggtcacgg gaacagcggt tgcggggaat acggtgattg tcactattgg
    15301 cggcaatcaa tacaccgcca ccgtgcagcc agatttaagc tggagcgtca gcgtaccggc
    15361 gaacgttttg caggcgctgg gcaacggaga actgaccatc agcgcctcgg tgaccaattc
    15421 cgcaaataat actggcaccg caacgcacga tatcgtgata gatgccaacc tgcctggcct
    15481 gcgggtcgat accgtggcag gtgatgatgt gattaacagc attgagcaca ctcaggcgtt
    15541 ggtgatcacc ggtagcggta gcggactggc ggcgggggcg gcgttgacgg tggttatcaa
    15601 tagcgtcacc tacggcgcaa cggtactcgc cgatggcacc tggagcgtcg gtgttcctgc
    15661 ggcagatgtc acaaactggc ctgcgggaac ggtcaatatt gccgtttctg gcaccaatac
    15721 cgccggaacc acaaccagca ttagccatcc ggtcacggtc gatctcgcgg ccgtggcgat
    15781 taccattaac accctttcca ctgacgatgt gattaatgcc gccgaaaaag gcagcgattt
    15841 gcagctctcc ggcaccacct ccgatgtgga agcggggcaa accatcaccg ttatcttcgg
    15901 cggcaaaagc tacaccacca cggttgccgc gggcggcacc tgggggctga caatcccagc
    15961 agccgatctc gcaaccttgc ctgacggcgc agcaaacgtc caggccagcg tcagcaacgt
    16021 cgcaggtaac agcgcccagg caacgcatgc ttacagcgtc gatgccaccg cgccatccgt
    16081 taccatcaac accatcgcca gcgacgatat tcttaacgcc gccgaagcgg gatcggcgct
    16141 gaccatcagc ggcaccagca cggcagaagc cgggcagacg gtgaccgtga cgcttaacgg
    16201 cgtcaattac agcggtaacg tgcaggcaga cggaagctgg agcgtcagcg tcccgaccgg
    16261 cgatttagct aacctcactg ccagccccta tacggtgagc gcagcggtca gcgacaaagc
    16321 cggaaatccg gcttcagcaa cacataatct gacggtggac cttgccgctc cggttgtcac
    16381 cattaacacg gtggcgggcg atgacatcat caacgccaca gaacatgcac aggcgcagat
    16441 tatcagcggc tcggcaacgg gcgcgactac cggtaacacg gtttccgtga ccattggcac
    16501 gaccacctac accaccgtgc tggacgccaa cggcaactgg agcatcggcg tgcctgccag
    16561 cgtgatttcc gcactggcgc agggcgatgt caccattacc gccacggtca ccgactccgc
    16621 aggcaacagc ggcacggcct cgcacactgt caccgtggcg ctcggcgctc cggtgctcgc
    16681 cattaacacc atcgccgttg atgacatcat taatgccacg gagaaaggcg cggatctggc
    16741 gatttccggc agcagcaacc agcctgcggg tacgcagata accgttacgc tcaacgggca
    16801 aaattacacc accactgccg atgcatccgg taactggagc gtgaccgttc cggcgtcacg
    16861 ggtgagcgcc ctcggtgaag ccacttacac ggtgaccgcc gccgctactg actctgatgg
    16921 caacagcggt tccgccagcc ataacgtgca ggttaatacc gcgctgccgg gcgtcaccat
    16981 taacgtggtg gcaacggacg atattattaa cgccgccgaa gcgggcgtgg accagaccat
    17041 cagcgggcag gtgacgggtg ctacggcagg cgacacggtg accgtcacgc tcggcggggc
    17101 gacgtacacc gccacggtgc aggcgaattt aagctggagc gtcgatgttc cggcagccgc
    17161 tttacaggcg ctgggcaacg gcgagctgac catttcggct tcggtaacga acagcgtagg
    17221 taataccggt aacggcacgc gtgaaatcac catcgacgcg aatctgcccg gtctgcgggt
    17281 cgataccgtg gcgggcgatg atgtggtcaa tattatcgag cacggacagg cgctggtgat
    17341 taccggcagc agttccggtc tggcgacgga cagtaacgtc acgctgacca ttaacgggca
    17401 aacctatgtt gcggcggtac tggcggatgg ctcatggagc gtcggcgttc cggcggcaga
    17461 tgtcagcgcc tggcctgcgg ggacggtcac gattacggcg agcggtagca ccacagccgg
    17521 aaatccggtc agcgttacgc atccggtgac tgtcgatctc actgcggtgg cggtcagcat
    17581 caacgccatt actgccgatg acgtgattaa cgctgccgaa aaaggcgcgg cgttaacgct
    17641 ctctggcagc acttctggtg tggaggccgg gcaaacggtc accgtcacct ttggcggcaa
    17701 aacctacact gcaagcgtcg cggcgaatgg ttcctggagc acctcggttc cggcggcaga
    17761 tatggcggcc ctgcgtaatg gcgatgccag cgcacaggcc agcgtcagca atgttaacgg
    17821 caacaacgcc actactaccc acgcttacag cgtcgatgcc agcgtgccaa cggtgaccat
    17881 taacaccatt gcgggcgacg acattcttaa cgccgccgaa gcgggtgctg cgctgactat
    17941 cactggcagc agcacggcgg aagcggggca gacggtgacc gtcaccctca atggtgcaaa
    18001 ctatactggc accgtacaga cggacggcag ctggagcatc agcgtaccgc cagccgactt
    18061 aagcgccctg accgccagca actacaccgt tagcgcggcg gtgagcgaca aagccggaaa
    18121 cccggcctcg gttaatcaca acctgacggt ggatacttcc gttccggtcg tcaccatcaa
    18181 cacggtggca ggcgatgatg tgatcaacgc gacggaacac gcccaggcgc agatcatcag
    18241 cggctccgcc acgggtgcgg caaccggtag caccgtgacg gtgactatcg gcacaaatac
    18301 cttcaccacc gtgctggatg ccagcggtaa ctggagcgtc ggcgttccgg caagcgtcgt
    18361 ctcggcgctg gcgaacggca cggtgaccat taacgccagc gtcaccgatg ccgccggaaa
    18421 cagcggcagc gccacgcatc aggtgacggt caatactggc ctgccgtcaa ttacctttaa
    18481 cgccatcagc ggcgataacg tcctgaacgc cgatgaaaaa ggccagccgt tgaccattag
    18541 cggtagcagt acggggttgg cgaccggcgc acaggttacc gtcacactca acggtcataa
    18601 ctacagcgct accaccgacg cggcgggcaa ctggacctta accgtgccgg tgagcgatct
    18661 ggcggcttta ggccaggcca actatatcgt cagcgccagc gccaccagtg cggcaggtaa
    18721 caccgccagc agccaggcga atttactggt cgacagcggc ctgccgggcg tcaccatcaa
    18781 caccgtggca ggcgacgata ttatcaacgc cgccgaagcg ggagccgctc aaaccatcag
    18841 cggggtggtg actcgcgccg ccgctggcga tacggtcacc gtgacgcttg gcggcaacac
    18901 ttatactgca caagttcagg ccgatttaag ctggagcgtc agcgttccgg cagccgacct
    18961 ccaggcgctg ggcaatggtg atttgaccat taccgcctcg gtcaccaacg cgaatggcaa
    19021 caccggcagc ggcacgcggg atatcaccat tgatgccaac ctgccggggc tgcgcgtgga
    19081 tacggtggcg ggcgatgata tcgtcaacag cattgaacac gggcaggcgc tggtgatcac
    19141 cggcggcagc agtggtctga atgccggtgt gccattgacg atcacgatta acggtacggc
    19201 ttattccgcc acggtacagg cggacggaag ctggagtgtc ggcattccgg cggcaaacgt
    19261 tagcgcctgg ccagcgggag cattaaccgt tgaggtagac gggcagagca gcgccggaaa
    19321 tccggtcggc gtcagccatc cgttcactgt cgatttaacg gcggtggcaa tcagcatcag
    19381 caccgttgcc agcgatgacg taattaatgc cgccgagaaa ggcaccaatc tgaccctttc
    19441 cggcagtacc agcgggattg agagcgggca aaccgtcacc gtcacttttg gcggtaaaac
    19501 ctacactgca agcgtcgccg ccaacgggag ctggagtgta aacgttccgg cggcagatct
    19561 ggcaattctg ccagacggcg cggcgaacgt gcaggctagt gtcagcagcg cgagcggtaa
    19621 cagtgcctcg gcgacccatg cgtatagcgt tgacgccagc gcaccgacgc tcaccattaa
    19681 caccattgcc agcgacgata ttcttaacgc cacggaggcg ggaagcccgc tcatcatcag
    19741 cggcaccagc accgccgaaa ccgggcagac ggtcaccgtc acccttaacg gcgcaaccta
    19801 cagcggtaat gtgcaggcag acggtagctg gagcgtcagc gttccccctt cagccttagg
    19861 cgcgctgagc gcaagcaatt acaccgtcag cgccacggtc aacgacaaag cgggcaaccc
    19921 cggcagcgcc agccataatc tggcggtaga caccaccgcg ccggttctca ccattaacac
    19981 tgtggtgggc gatgacatta tcaacgatgc cgaacatgcg caggcgctgg tgatctccgg
    20041 caccagcacg ggcggggaag cgggcgatgt ggtgagcgtt gtgctcaacg gcaaaaccta
    20101 caccaccacc ctggatgcct ccggtaactg gagcgttggc gttccagcgg cggatgttgc
    20161 ggcactcggt agcggtgcgc agaccatcac cgccagcgtc agcgatcggg caggcaacag
    20221 cgacgacgcc agccgcaccg tgaccgtcag tctcaccgcg ccagtgatta gcatcaacac
    20281 catcgcgggc gatgatgtga tcaacgcgac ggaaaaagga tctgatctgg cgctttccgg
    20341 catcagcgat cagccagcgg gcacggcgat caccgtcacc ctgaacggac aaaactacag
    20401 cgccaccacc gactcttctg gcaactggag cgtcaccgtg cccgcctcgg cggtcagtgc
    20461 gctgggcgaa gcctcctaca gcgtgacggc gagcgtcacc aatgctcagg gtaacagcag
    20521 caccgccagc cataacgtgc aggtcaatac cgcgctaccg ggcgtcacca ttaatccggt
    20581 aacgacggac gatattatta acgccgcaga agcaggcagc gcgcaaacta tcagcggcca
    20641 ggtaaccggt gcggcggcgg gcagcaccgt caccgttgaa ctgggcggta aaacttacac
    20701 cgccaccgtc caggctgatt taagctggaa tgtgagcgta cctgccgccg actggcaggc
    20761 gctgggtaac ggtgagttaa cggttaatgc ctcggtgacc aacgccgttg gtaacaccgg
    20821 tagcggcacg cgcgatatca ccatcgatgc cagtctgcct ggcctgcggg tggataccgt
    20881 cgcgggtgat gatgtggtca atatcatcga acacagtcag gcgcaggtga tcaccggtag
    20941 cagctccggc tttgcggcgg gcacggcgct gaccgtagtg ataaataatc aaacttacgc
    21001 cgccacggtg ctggcgaacg gcagctggag cgtcggcgtt cctgcgacgg atgtcagtaa
    21061 ctggcctgcg ggaacgctga atattaccgt tagcggcgcg aacagtgccg gaacgcaaac
    21121 cagcattacc catccgctga ccgttgatct caccaccgtc gccgtcagca taaacagcat
    21181 caccagcgat gatgtgatta acgccgccga aaaaggcgcg gcgctaacgc tctccggcag
    21241 cacgtccggt gtcgaagcgg ggcaaaccgt taccattact tttggcggca aaacctacac
    21301 caccacggtg gcggctaacg gtagctggag caccaccgtt ccggctgtgg atatggcgac
    21361 cctgcgtgac ggcgatgcca gcgcccaggt gcgggtgact aacgtcaatg gcaacagcgc
    21421 cacggcaacg catgaataca gcgtcgatag cgccgcgcca acggtgacca tcaacaccat
    21481 tgccagtgat aacatcatca acgccagcga agcggcggcg ggcgtgacgg tatccggcac
    21541 cagcacggcg gagacggggc agacgctgac cgtcacgctt aacggcacta actaccagac
    21601 cacggtgcag gcagacggca gctggagttt aacgctgccc gccagcgact taaccgcact
    21661 tgccaataac ggctacaccc tgaccgcctc ggtcagcgat ttggcgggta accctggcag
    21721 cgccagcaaa ggtgtgaccg tcgataccac cgcgcccgtc atcagtttca acaccgtggc
    21781 gggcgacgat gtgattaaca acgtcgaaca cactcaggcg cagattatca gtggcaccgc
    21841 cacaggcgcg gtggcgggcg accgcctggt ggtgaccatc gccgggcagc agtatgtcag
    21901 cagcaccgat gccagcggca actggagcgt tggcgtgcct gccagcgtga tttccggcct
    21961 tgcggacggc acggtgacca tcagcgccac cattaccgat agcgcaggca acagcagtac
    22021 gcagacgcac aacgtgcagg taaacacggc ggcagtgtcg ctctcggtca gcaccatcag
    22081 cggcgataat attattaacg ccgccgaagc aggcagtgcg ctgaccctga gcggcaccgg
    22141 aacgaacttt gccgcaggta cagtagtgac cgtgttgctt aacggcaaag gctacagcgc
    22201 caccattcag agcaacggga gctggagcgt gaacgtgccg gcggcagacg tcgcagcact
    22261 cagtgatggc accagctaca cggttagcgc ctccgcgcag gacagcgccg gaaacagcgc
    22321 cacggcctca cgcagcgtgg cggtggatct caccgcgccg gttatcagca taaacacggt
    22381 ttcaacggac gaccgactca acgccgccga acagcagcag ccgttaaccc ttaacggctc
    22441 gactagcgcg gaagtggggc agacggtcac cgtcaccttt ggcggcaaaa cctataccgc
    22501 cacggttgcc gccaatggca cctgggcgct taacgtgcct gcggcggatc tggccgcgct
    22561 cgggcagggt gcgcagacca ttaccgccag cgtgaacgat cgcgccggta accccggtca
    22621 aaccacgcac gccctgaccg tcgataccgt tgcgccaacg gtcaccatcg ccacggtggc
    22681 gggtgacgat attatcaaca acgccgagca gcttgccggg cagaccatca gcggcaccac
    22741 caccgccgaa gtgggccaga cggtgaccgt tacctttaac ggtcagagct ggactgcaac
    22801 ggtcggcagc ggcggaagct ggtcggtgtt tattccggcg cagcagtttg ccggattaag
    22861 cgacggcagc tacaccatta gcgcgacggt tagcgatcag gccggaaacc ccggcagcgc
    22921 cagccgtggc gtgacgctga acggcgacgt cccctccgtc accatcaaca cctttgcggg
    22981 cgatgatgtg gtgaatgcag cggagcacgg ctcgtcgctg gtgatcagcg gcaccaccac
    23041 cgccccagtc gggcagacgc tgaccctgac cttaaacggc aaaacctaca ccaccacggt
    23101 acagaccggc ggtagctgga gctacaccct cggcagcgtc gatgtcaccg cgctggcgga
    23161 cggcaatgcc tacgtgatta acgcctcggt gagcaacgct attggcaaca ccggcagcag
    23221 caatcacacc attactgtcg atctcagcgc cccggcgatg ggtattaata tcgattccct
    23281 gcaagccgac actggcctta gctccagcga ctttatcacc agcgtcagtc cggtggtggt
    23341 caacggctcg ctcaccgccg cgcttgccag taacgagacg gcacaaatca gtatcgacgg
    23401 cggcgtaacc tggaccacgc tcaccgttac cggcacgatc tggcgctata acgacagccg
    23461 cacgctgacc gatggcagtt atctctatca ggtgcgggtg attgacgcgg cgggcaacgt
    23521 cggtgccact gacagccaga atgtggtgat cgacaccatc gcgccagatc ccgcggtgaa
    23581 aaccatcgcc atcaacgcga tcaccaccga tactggattg attactaacg attttgtcac
    23641 cagcgacacg acgcttgcgg taagcggcac gctgggggcg gcgctttctt ccggtgagtt
    23701 cgcgcaaatc agcattgatg gcggcaccac ctggcagaat ctttcggtaa gcggtttgac
    23761 ctggacatat cttgacgggc ggacgctgag cgatggcaac tacaactatc aggtgcgggt
    23821 tatcgacacc gccggaaata tcggcgcgac cgccagccag atagtcactg tggacaccac
    23881 cgcgccgctg gcgagcaaaa ccatcgccat tgcgggcatc agcgatgaca ctgggttaag
    23941 tagcagcgat tttgttactc gcgacaccac gctgaccgtg cgcggcacgc tcggcgcggc
    24001 gcttgccgcc gatgaacggg cgcaaatcag cctcgacggc ggtgtcacct ggaccacgtt
    24061 aaccgttatc ggcaccagct ggagctatgc cgatggtcgc acactgaccg acggcacctg
    24121 gaactacacg gtgcgggtgg tggatctggc ggggaacgtt gggcagaccg caacgcaaaa
    24181 tgtggtggtc gacaccacca gcccggaagc ggcgaaaagt atcaccatta ccggtatcag
    24241 cgatgacacc ggagccagca gcagcgattt tattaccagc gacacctcgc ttaccgtgcg
    24301 cggcgtattg ggggcggcgc tcggcgctaa cgagttcgcg caaatcagta ccgacaacgg
    24361 cgcaacctgg gtgaacgtaa cgctggcggc agatggcctg aactggagtt acgttgacgg
    24421 acgcacgcta acgaatggca cgacgacctg gcaggtgcgg gtggtcgatc tggcgggcaa
    24481 cgttggcgca accagcagcc agtcggcgca gatcgacacc gttaacccgg cgcaggtgtt
    24541 gaccatcgcc agcatcagca ccgacacggg gagttcggca actgacttta tcaccagtga
    24601 caccacgctc accctaaccg gttcgctggg ggcggggctt gccagcggcg aagtggcgca
    24661 gattagcctc gatggcggcg cgacctggat cacactcacc accaacggca cgcagtggac
    24721 ctacactgac agccgcacgc tgaccgacgg cagctatgtt tatcaggtgc gggtgctgga
    24781 tctggcgggg aacaccggct cggtggtgtc gaaaacggtg gtggtcgata cgattaaccc
    24841 taccgccacg ccgaccattg tgtcgtatac cgatgatgtc gggcagcggc aggggacatt
    24901 aagcagttcg caggccaccg acgacactac gccgttgctg aacggcgtgc tctccggccc
    24961 gcttgccagc ggtgaagtgg tttacctcta ccgtaacggc ctgctgttag gcgcggtaac
    25021 gatggtcggc gcactgaact ggacctacag cgacagcggg ctggtgagcg gtgcctacac
    25081 ctacagtgcg cgagtggtgg atctggcggg gaatatcacc gcctccagtg attttgtcct
    25141 gacggtcgat acctctattc cgaccacgct ggcgcaaatt accagccaga ccacgcgcga
    25201 taccacgccg attatcagcg gggtaatcac ggcggcactc gccagtgggc agtatgttga
    25261 agtggtgatc aacggcaaaa cctacacctc tgaaccgggt ggcgcggtag tggtcgatcc
    25321 ggcgcacaac acctggtatg tacagttgcc ggataccgac gcgctggcgg cttctgcgac
    25381 cgcctataac gtcaccgcgc aggtaaaaag ctcggcgggc aacggcaata acgccaatat
    25441 tagcaacggc acggtgacgg ttaacgcggc gattgattac acaccgacct ggaccaccgc
    25501 cagcaaaacc accgcctggg gattgaccta cggccttgac tcgcacggga tgtggacggt
    25561 gctggcaaac cagcaggtaa tgcaatcgac tgacccactc acctggtcga agaccgcgct
    25621 gacgctgtat cagagcggca acaactacgc caccagctcc atcgccgatt acgaccgtaa
    25681 cggcacgggc gatctgttta tcacccgtga tgactacggt acaggctata tcaacggctt
    25741 taccaataac ggcgatggca ccttttccag cgctattcag gtcaccgtcg gcaccctgac
    25801 gtggtacggc tcgattgtgg catttgataa agagggcgac ggctatctcg acttctggat
    25861 tggcgacgcg ggcgggccgg actccaacac cttcttgtgg aacaacgcag gtacgctggt
    25921 aggtaactcc accacgtcga acagcggcgg tagcgccacg gtgggcgggg cggtgacagg
    25981 gtatctttcg ctcaacgaag gttctggtgt cgatctgaac aatgacggca ggatcgacct
    26041 ggttcagcac acctacaacc tgaacaacta ttacacgcta tcttcgctca tcaaccaagg
    26101 gaatgggacg tttgtctggg ggcagaacac caccaatacc ttcctgagcg gggcgggcag
    26161 tggcgctatg agcagcagcg tttccatgac ctgggccgat ttcgatggtg acggcgatat
    26221 ggatctcttc ctgcctgcca gccagggaag agctaactac ggctcgctgt tattcaacac
    26281 caacggcgta ctgggtagcc cggtggcggt gggcgcaacg gcaaccacct atgccagcca
    26341 gtttagcgtg gcggtggact ggaaccatga cggcctgatg gatatcgccc gtatcgccca
    26401 gaccgggcag tcgtatcttt ataccaacgt cagcaatgcc agcaactgga cacaatcggc
    26461 cctcggcggc agccagagcg gtaccaccag cggcgtggcg gcaatggact acgactggga
    26521 cggcgcggtg gatgtgctgg tgaccaaaca gtcgggcagc gtctatctta ttcgcaacac
    26581 caacacggtg agctacggca cctcgctgca cctgcgcatc accgatccta acggcattaa
    26641 cgtctattac ggcaataccg taaaactgta caactcggcg ggagtgctgg tcgccacgca
    26701 aatcatcaac ccgcagtcgg ggatgggggt taacgacacc tcggcgctgg tcaacttcta
    26761 cgggctgaat gccggagaaa cctacaacgc ggtgctgatc aaatccactg gcaccaccgc
    26821 cagcaatatc gaccagacgg tcaacaccag ctggggcggc ttacaggcca ccgatgccac
    26881 tcacgcttac gacctcagcg ccgaagcagg caccgcgagc aacaatggca agttcgtcgg
    26941 caccggctat aacgacacct tctttgccac cgcaggcacc gacacttacg acggttccgg
    27001 cggctgggtg tacagctccg gcaccggaac ctggcttgcg aacggcggca tggacgtggt
    27061 tgatttccgc ctctcgacgg tgggcgtgac cgcgaactta agcagcaccg ccgcgcaggc
    27121 caccggcttt aacacctcga catttaccaa tatcgaaggg atatccggct cgaattttaa
    27181 cgacactctg accggcagca gcggcgataa ccaactggaa gggcgcggcg gtaacgacac
    27241 gctcaacatc ggcaacggcg gccacgacac cttgctctat aaactgctca acgcaagcga
    27301 cgcgaccggc ggcaacggct cggatgtggt gaatggcttt acggtaggga catgggaagg
    27361 caccgccgac accgatcgca ttgatatccg cgagttgtta cagggcagcg gctacaccgg
    27421 caacggcaaa gccagctacg tcaacggcgt ggcaacgctg gatgcgcagg ccggaaacat
    27481 cggtgatttt gtcaaagtca cccagagcgg cagcgacacc atcgtgcaga tcgaccgcga
    27541 cggcacgggc ggcacttttg cggcaactaa cgtggtcacg ctgacgggcg tgcataccga
    27601 cctcgccact ttgctggcga atcatcagtt gatggtggtg tagagaatga aaaaaaatgc
    27661 ccagtccgtg gaagcctggc tggaggcgat gatcgccgtg gcgcgctatt accgccttga
    27721 tttttctcag gagaatgtgc gcgcgaccgt caactgggag cgcgacagta agcgggagga
    27781 gttactcacc gacatggcgc ggcagttggg gatggggctt cggctggtgg agttctccac
    27841 cgactcgctc aacccctggc ggctgccgtt gatcgccgtt tttgataatc aacaaattgg
    27901 cgtgatcacc cgccgcgata atcacgacaa tatcagcgtt cagttcagcg gtgacgaagg
    27961 gctggaaacc acgcttaacg ttacggatat tgaagataac atcgtcgaac tggcgctgtt
    28021 gcgcccgctc agcgctattc ctgacgcgcg ggtagacgac tatattcgcc cgtatcaggc
    28081 gaactggttc tggaatctct cgctaaaaga ctggcggcgc tatggagata ttatgctggc
    28141 gtcactggtg gcgaacgtgc tggcgcttgc ggcgatgatt ttctcgatgc aggtttacga
    28201 ccgggtggtt ccggcgcagt cctacccgac gctgtgggtg ctgttcgccg gggtgatgat
    28261 ggcgatcctg tttgaattct gtatgcgcat ggtgcgcacg cacctttccg atgtgattgg
    28321 taagcgggca gatctgcgca tttcggatcg cgtttttggt cacgcattac ggctgaaaaa
    28381 taacgtgcga tcgaaatcca ccggatcgtt tatctcgcag atccgcgaac tggaatcggt
    28441 gcgggagctg atcacctcca ccactattgg tgcagtggcg gatctgccgt tcttcctgct
    28501 gtttgtcttt attttgtgga tgattggcgg ctggctggtg ctggtggttt tgctggcgct
    28561 gccgttattg gtgatccccg gcctgctggt acaacgcccg ctggcgcggc tggcgaacga
    28621 agggatgcgc gagtcggcgg tgcgtaacgc tacgctggtg gaagccgtgc agtcgattga
    28681 agacattaaa ctgctgcgcg ctgaacagcg ttttcagaat cagtggaatc acactaacga
    28741 cgtggcctcg tccatcagca tgaagcaacg ttttctcacc gggctactgc tcacctggac
    28801 gcaggaagtg caatccatcg tctacgtcgt ggtgctgttg gtgggctgct ttatggtaat
    28861 gaacggtgac atgactaccg gcgcgctggt cgggacgacg atcctggctt cgcgcaccat
    28921 cgcgccgctg tcgcagatat ccggtgtgct ctcgcgctgg cagcaggcga aagtggcgcg
    28981 caacggactt gatgaactga tgaagcgccc ggtggatcag ccggaacacg gcaaactggt
    29041 gcataaagcg gtgctacacg gcaattatca gttcagcaac gctatctttt attacgatga
    29101 agaagagaaa attgccgatg tggcgattgg caaactcaac atccaggccg gagagaaaat
    29161 cgccattctc gggcgcaacg gcgcgggtaa atcaacgcta ctgcaaatgc tcgccggaat
    29221 gcgtatcgcc cagcaggggc aggtgctact ggataacatc agtatcgggc agcttgatcc
    29281 ggcagatctg cgccgcgaca tggggctact gagccagacc ggacgactgt ttttcggctc
    29341 gctgcgggaa aacctgacga tggggatgcc ggaggccagc gatgaagata tcgaacgcgc
    29401 attaaccctc agcggggcgc tgccgtttgt gcagaagcag aaaaacggcc tcaactacat
    29461 gattcaggaa gggggatttg gtctttctgg cggtcagcgg caaacgctgc tgctggcgcg
    29521 gcttctcatc tctcaaccca atatcgtgct gctcgatgaa cccagcgcct cgctggatga
    29581 aatggcagaa gcgtatttaa tcgaacaact gaaacagtgg attggccatc gcacgctgat
    29641 tatcgccact caccgcacgg cgatgctgca actggtggac cgcattatcg tgatggatca
    29701 ggggcggatt gtgatggatg gcgcgaaaga ggccattttg cgtgaacagg gcgaacccac
    29761 gacgcgacgg gtggtgttgc aggagaagaa taaggggtct gccgcatgaa tggacaactg
    29821 caatacaacc gtggcctgac tgaaccccgc ctgccgcgtt cggcgctggc ggtacgcgtt
    29881 accgctgtga tgctgctctg ttttctcggc tgggcgtggt acttccagct tgatgaagtc
    29941 accaccggca gcggcacggt agaaccttcc gggcgcgaac aggtggtgca gtcgctggaa
    30001 ggcggaattc tgtatcatct cgacgtcaag gttggcgata ttgttgaaca gggccagccg
    30061 ctggcgcagc ttaaccgcac caaaacggaa tccgacgtcc aggaagcaat gtcgcggctg
    30121 tacgcggcgc tggcaacctc ggcgcggctg cgggcggaag tgagtaataa accgctggtc
    30181 ttccctgacg aactgaataa attccccgaa cttatcgagt cggaaacggc gttgtacaac
    30241 actcgccgcg atgggctgaa caaagccacc accgggctga cgcaggggat tagcctggtc
    30301 aaccgcgaac tggcgatgac ccagccgctg gtaaaacagg gggcggcgag cagcgttgaa
    30361 gtgctgcgcc tgcaacgcca ggccaatgaa ctggaaaaca aactcagcga cgtgcgcacg
    30421 caatactacg tacaggcgcg ggaagagtta gcgaaagcca atgcggaagt agaaacccag
    30481 cgttcggtga tccgcgggcg ggaagattct ctcacccggc tcaactttac cgcgccggta
    30541 cgtggcattg ttcaggacat tgacgtcacc acggttggcg gcgttattgc ccccggtggt
    30601 aaactgatga ccatcgtgcc gctggatgag caactgctga tcgaggcaaa aatctccccg
    30661 cgcgatgtgg cgtttatcca tccggggcaa aaatcgctgg tgaaaataac cgcctacgat
    30721 tactccatct acggcggcct gccgggagaa gtggcggtga tatcaccaga caccgtgcag
    30781 gatgaagtac ggcgcgatgt ttattactat cgggtgtaca ttcgcacttt cagcaaccat
    30841 ctggaaaaca aaagcaaaca gcaatttccg atatttcccg gtatggtggc gacggtcgat
    30901 atccgcaccg gcaaaaaaag cgtgctggat tatctgttga aaccctttaa caaagcacag
    30961 gaggcgttgc gcgaacgatg aacattagcg atgtagcaaa aattaccggc ctgaccagca
    31021 aagcaatccg cttctatgaa gagaaggggc tggtgacgcc gccgatgcgt agcgaaaatg
    31081 gttatcgaac ctacacgcag cagcatctca acgaactgac cttactgcgc caggcgcgcc
    31141 aggtgggctt taacctggaa gagagcggcg aactggtgaa tctgtttaac gacccgcagc
    31201 gtcacagcgc cgatgtaaaa cggcgcaccc tggagaaagt ggcggagatc gaacgacaca
    31261 ttgaggagct gcaatccatg cgcaaccagc tgttggcgct ggcgaatgcc tgccctggcg
    31321 atgacagcgc cgactgcccg attatcgaaa atctctccgg ctgctgtcat catcgggcgg
    31381 ggtgaaaatc agttaatccc cgtttgcacc gaaagaatat gctgcaaact ttctaccata
    31441 atgcgccccg actgaaataa gccttcctgg atttcgttgt taacattcac agcgtgaacc
    31501 agggaggcta ataatactac ttgcaagaag gcaaaaataa acgcaccgat aatcataata
    31561 tgcgtcaggg gtattttcct ttgctccttc attttaaata acccacaaat tgaccttcct
    31621 tcattaaggc gacttttcct gtgtgatgat aaacaatgac gttatacaga cctgactgat
    31681 aaacagtggc atctttattg tcttcatcaa ttgcacataa tgaatcattg agataagcaa
    31741 agtttttact gctcattcta aaggtcattc caaatccttt ataaatggct ttctttgccg
    31801 cctgggcatg ttgtgcggct tctgcatttt taaatgcctg cgcctcggat gcacttaaaa
    31861 tagccgtctg acgctgttgt tctgcgagat aacaggtgtc atggctgacg ccctgcgcct
    31921 ggcaatctgc gatgcgggaa gtgggtgaac tacagccagc cagtaatata accgccagca
    31981 gggaaaacga tttaatggag ctattcatca ttgttaacct taaataatgt acggaaaatt
    32041 gcgaataaga aattgctgtt aaaattagaa cgaatatccc acgccgacac ccgcaccgaa
    32101 atcagattgt gtatccgcag aaatacctgc tttaagtacc gtatgcgttc cggcattaaa
    32161 agatgttcct acagacaccg cagattcacc gttgaaggtt cccgctcccg ctgacaccat
    32221 catcaagtca ccggttttca cctgtggttg tgaggcgata gcgatggctg atgcagttcc
    32281 cgcattggct tctttacgat tgtcctcaac ttcgtttttc agagaatcga aatggttatt
    32341 gagcgtgtta atttgttggc tgttatctgc gacccgtttt tgcgtgttgg ttaactgttg
    32401 ctgcgtttgt gcggaatctt gtctcagact ttcaatttgc tcatgtgcct ggacagactt
    32461 ctgcgccaga ctttgcattt gtttatcgta ttgagccctt tctttttgct gttgtgctgc
    32521 cacatcggtt gtcagtgcat tcattttctg cccttgttcc gtggtagtgc gctgattagc
    32581 ctcattttga gccgccagtc tggcattaat catctcctga taatgcgcag tattttgcgt
    32641 atctccagtc gcggcgagcg ttttttgtgt ggctacgatc tgcgcctgtt gctctgtaat
    32701 ttgcgtttgc tgggcatcag ttaccgcaga ctgtcgggcg ttgatcatgc tctgataacg
    32761 ggcggttgtc tgtacatcac ctgtagcggc aacggttttt tgtcctgcgt caggatctgt
    32821 ctggacagtg ggagtctgag tggtatcagg tgtttcagaa gggatgtagt gatgttcagt
    32881 caaataggta tacgcagcgg ggctgttgtc tgtgcgttct aaaaagcggg taatcttctg
    32941 ctcaatctcc gattgcgaca tgccagagtt aatggctgaa agctctgttg ccgccgcttc
    33001 aatggtatca ccagctaaaa caacagggct ggatgttgct gaaattatga gtgccagtaa
    33061 agctacgttt acagttttca ttcattttct cttgttcaaa ataagtcgca ttaatgtttc
    33121 tataagaaac aaataatatt gttttaagaa ataaactgcg ttgcaatttg atcgaaatct
    33181 acgccgccat ttgtgtgatg taaagcgcat ttttgtctgg tgtaagcgta aacaccacca
    33241 ataagaaaca aaaaatgttt cttatataaa taaaatcgaa aatcattttg tgctctttca
    33301 atgtaaaaaa gagtaaagac ttgttttgat aagcgaaata aattctgttt ctggactaaa
    33361 cgtgaagcgg ttcctgatgt gtgctaaaat gcgtttcttt gataaatctc aatttttaag
    33421 aggtttacgc caggcaggaa aatttaaatg aatgaatttg agaagatttt taatgaaatg
    33481 aatctggacc gggcattgct accgatcctt tttcgttcga accggtcaac agtgtggaaa
    33541 tatttgagtg gtgatagtac ggctcctgcc tcagctatgt cactgattat gctgttgcag
    33601 ttaatccaga aacgaaaccc cgatctgtta gccgaatggt taacgctttc tgatttcacc
    33661 attccgccag aagtttacct ggaccagcct gattactgga aagggtgggt gtatacgcaa
    33721 cacaaggtca ataaaaacgt cctggaatat ctaaaaaaac attatccgga tgaagaccaa
    33781 aaaaacatga ataaaggtag tgacgagtaa tttgccaatg ccagttaata tagcggaggc
    33841 ggcctacgat tcgggcgcag acttccaggc atgataagac gcgcccgcgc cgcatcaggc
    33901 atcagcgcat aaccgccgga tgcggcgtaa acgccttatc cggcctacaa ttcgggcata
    33961 ttttgtaggc atgaaaagac gcatcagcgt cgcatcaggc gatgaccgca cggataatca
    34021 gcgttatccc ttctatcgca atcacctcaa catgcgtacc tgcactgaga tcctcgctgg
    34081 cgctgacagg ccatgaactg tcgccgacgc gcatatgacc tcgcccattg accagcggtg
    34141 attccagcac aaaacgtcga ccaatcagct gctgcccccg ctggtttaaa tgactatcgc
    34201 tgtgcttttg ttcgcgcacc cgccgcgaca accatttcca ccacagccag gcggcgagca
    34261 gcgtcaggat ggcaaacatt actccttgcc actcccaacc cagcggcacc agccagacta
    34321 ccaggccggt aatcaccgcg gccacgccgc tccacaacaa ataaccattt ccgcccagca
    34381 tctcggctgc cagcaacaac ccgccgagac tgagccagaa aatatgtgga tgaacgacca
    34441 ttaactccat catggctgag tccgtttgtt ggcgctgtct ttcaccagct cggcaatccc
    34501 ggcaatcgac cccatcaggc tgctggcctc taatggcatc atcactactt tgctgttact
    34561 ggaggaaccg atctgctgta acgcttcggt gtatttctgc gctacgaagt agttcaccgc
    34621 ctgaatatca ccggaggcga tagcttcaga caccattttg gtggcgcggg cttctgcttc
    34681 ggcagaacgt tcacgcgctt cagcctgtaa aaacgccgac tgacgttcgc cttccgcttt
    34741 caggatttgc gactgttttt caccttcagc tttgaggatt tccgcctgac ggatcccttc
    34801 cgcttcgagg atgtaagcgc gtttggtacg ttctgctttc atctgcgcgt tcattgaaga
    34861 gataagctct gccggtgggc gcacgtcgcg aatttcaata cgggtgactt taatccccca
    34921 cgggttagtg gcttcatcga caatacgcag caggcgtgag ttgatgctgt cgcgctgaga
    34981 gagcatttcg tcaagttcca ttgaacccag cacggtacgg atgttagtca tggtcaggtt
    35041 gatgatcgcc agctccagat tgctgacttc ataggcagcg cgtggcgcgt caatcacctg
    35101 aataaaacag actgcgtcga tggtaacgtt ggcgttatct ttcgagataa cttcctggga
    35161 agggatatcg agcacttgct ccatcatatt gatcttgcga ccaatgcgat ccataaacgg
    35221 caccaccaga cttagcccag gctgtaacgt tttggtatag cgaccaaagc gttctactgt
    35281 ccactgatag ccctgcggca cgattttgac gcccgcgccg acaatgacca gcgcgacaaa
    35341 aatgagaatc gggataaaga taagcatcgg aaaaacctcc tgttgtaccg tccataatca
    35401 gcaaaattgc tgcttgatta aacaaattat acctgattac tgaaagagag ttccccctta
    35461 ttcctgggaa ggataaactg tttttagtaa aaatcagaaa aagggaacag cgatgcagga
    35521 aaatagtcct ttgcttcagc tacaaaacgt aggatatctg gcgggtgatg cgaagattct
    35581 taataacatc aatttttcgc tgcgtgctgg cgaatttaag ttaattaccg gtccttctgg
    35641 ttgtggcaaa agtacgctgc taaaaatagt tgcttcattg atcagcccaa ccagcggaac
    35701 gttactgttt gaaggtgagg atgtcagcac actaaagcca gaaatctacc gccagcaagt
    35761 ctcttactgc gcccagacac cgacgctgtt tggcgatacg gtatacgata atctgatctt
    35821 tccctggcag atccgtaacc agcagcctga cccggccatt tttctcgatt ttctcgaacg
    35881 cttcgcttta ccggacagca ttttgacgaa gaatatcgcc gagctatctg ggggtgaaaa
    35941 acaacgcatc tcattaattc gtaacctgca atttatgccg aaggttttat tgctggatga
    36001 aatcaccagt gcgctggatg aaagtaataa acataacgtt aatgagatga tccaccggta
    36061 tgtgcgcgag caaaatattg ccgtgctgtg ggtgacacac gataaagacg aaattaatca
    36121 tgcggataag gtgattacac tgcaaccgca tgccggagaa atgcaggaag cacgctatga
    36181 actcgcataa tattactaac gaatcattgg cactggcatt aatgctggtg gtggtggcaa
    36241 tcttaattag ccataaagaa aaactggcgc tggagaaaga tattctctgg agcgtcgggc
    36301 gagcgataat tcagctgatt attgtcggct atgtgctgaa gtatattttc agcgtggatg
    36361 atgccagcct gacattattg atggtgttat ttatctgctt taatgcggcg tggaacgcgc
    36421 aaaaacgcag taaatatatt gctaaagctt ttatctcatc gtttattgcc attacggtcg
    36481 gggcgggaat taccctggcg gtgctgattc tctctgggtc gattgaattt atcccgatgc
    36541 aggtgatccc tatcgccggg atgatagccg gtaacgccat ggtagcggtg gggttgtgtt
    36601 acaacaattt agggcaacgg gtgattagcg aacagcaaca gattcaggag aaactgagtc
    36661 ttggcgcgac gccgaaacag gcttcagcga tattgattcg cgacagtatt cgcgcggctt
    36721 taattccgac ggtcgattca gcaaaaacgg ttggcttagt gagtttacca ggaatgatgt
    36781 ccgggctgat atttgccggg attgatccgg tgaaggcgat taaatatcag attatggtga
    36841 cctttatgct gctctcaact gccagcctgt ctaccattat tgcctgctat ttaacctatc
    36901 gtaaatttta taattcgcgc caccagttgg tggtgacgca attgaagaag aaatgatgca
    36961 atgccggatg cgcagcatcg catccggcgt tgtggttcat gtgccgatca acggaaactc
    37021 aatacaacaa cgcatacagc tggcggcgat acttcgacgc cagtgcatca cctgtaccca
    37081 gcgcagcgag gatctcctgg aacgttttac gcgtctgacc gtctgcggca gtgagatctt
    37141 tacgcagatg cccaaacagt aactccagcg cctcttcatt gcgcccaacc tgatgcagtt
    37201 gcagcgccag ttgcgtcgcc agtgcggcat cttctggatt ctctgccacc tgctgttgca
    37261 gctgttgaat ttccggcgta tcagccgcct gcttcagcag ttcgatttgc gccaccagcc
    37321 cctgatagcg ggtgtcctga tcctgtaacg gaatggtttt cagcacggct tcagcatctt
    37381 cagaacggtt cagcgcaatc agcgtttctg ccagcagcag gccgatctcc ccgttctgat
    37441 tcgacaactg ccaggcgtct ttcagcaatg gcagggcgtc ggtgtaattg ccttcttgca
    37501 tcagttgcat cgcctgctgt gctttcagtt cttcttcgcg cggcagcact ttatccagca
    37561 gggcgcggat cgcctcttcc ggttgcggcc cctggaagcc gtctaccggt tgcccgttct
    37621 ggaacagata cacggtcgga atcgcgcgca gaccaaactg ggcggcaatc atctgctccg
    37681 cgtcgcagtc cagcttcgcc agaataaatt gcccgttgta ctgcgccgcg aggctttcca
    37741 gaattggggt taactgcaaa cagtgctggc tacgttcaga ccaaaaatag aacagcaccg
    37801 gagtggtcat cgactgttca agaacctgtt gcaggttaga ttcgttaatg ttgacaatat
    37861 tttctacgga catggagtcg ctctctgttg tcgatatttt ctttgacatg ggggcttaag
    37921 cgcgcgcttc aacttacccc tgcaatattt tgtccatcac gcgccccggc agcaggcgct
    37981 taagcaccat tactgcccag gtcaccagcg ttaccggata gcgcatcttc ggtttctcgc
    38041 taataaaagc atggcgcact ttgtcgacca ccgcttccgg ccccaacgta aagcgggcgg
    38101 cgatgccggg attttcgaca ggtttatcac tttgcgtctg gttgacgttg tcggtgaagc
    38161 gagtacgaat gggaccgggt tcgatcaggc taactttaat tccgctgtgg cgtagctcca
    38221 tgcgcagcgc atccgaccac gcctccagcg catatttact ggctgcgtaa gcgccgcgac
    38281 ccggcgtgga gattaatccc atcaccgatg aggtcatcac aatacgccct tcaccgtgcg
    38341 gtaacatcgc gggtaacagg cgcatggtga gctggtgtgc gccgaaaaag ttggcagaaa
    38401 actgctgttc catctgcgca cggctgatgg tggaaagggg gccatacatg ccgaatccgg
    38461 cattgttaaa gatcccatac agacaattat cggtcagggc gatcacttcg tcggctgcgc
    38521 gatcaacact ttctggtgaa tccagatcga tcaacacgcc ggtaaatccc atgctgttca
    38581 tgcgctcaac atcatccggt ttccggcaac ctgccagcac atgaaaaccc tggcgtttta
    38641 attcgagcgc gctttcctgg ccaattccac tggaacatcc ggtaattaag accgattttt
    38701 gcataacttt acctgtcagg atctccgttg ctttatgagt catgatttac taaaggctgc
    38761 aactgcttcg ccatccagtc ggcaataaac ggctgggcgt cgcggttggg atgaataccg
    38821 tcatcctgca tccattgtgg cttgaggtag acctcttcca taaaaaaggg cagcagcgga
    38881 acatcaaact ctttggcgag tttggggtaa atggcgctaa aggcttcatt ataacggcga
    38941 ccatagtttg caggcagacg tatttgcatt aacaatggtt cagcgttggc ggctttgaca
    39001 tcctgcaaaa tctggcgcag cgtttgctcg gtttgttgtg gctgaaaacc acgcaaaccg
    39061 tcattgccgc ccagttcaac cagcacccag cgcggctgat gctgtttcag cagagccgga
    39121 aggcgcgcca gcccttgttg cgaggtgtcg ccgctgatgc tggcattaac taccgacgtt
    39181 ttactctgcc acttatcatt caacaaggca ggccaggccg cgctggcaga cattcgatac
    39241 ccggcgctca ggctatcacc cagaatcaat aacgtgtccg ctgcggcggc acggaaggtt
    39301 aacaggacca ggaacaggaa gggcaaatgc cagcggaaaa cattgttgaa gttcatcatc
    39361 ttaagaagtc cgtcggtcag ggggagcatg aactctccat cctcaccgga gttgagctgg
    39421 ttgtcaaacg tggcgagacc atcgcactgg tgggtgagtc gggatcgggt aagtcaacct
    39481 tgctggcaat cctcgccggg ctggatgacg gcagcagtgg cgaagtgagt ctggtgggac
    39541 aaccgctaca taatatggac gaagaagcgc gggcaaagtt gcgcgcgaag cacgtaggtt
    39601 ttgtttttca gtcatttatg ttaattccta cccttaacgc gctggaaaac gtcgagcttc
    39661 cggcgctgct gcgcggtgag agtagcgcgg aaagtcgtaa cggggcgaaa gcgttgctcg
    39721 aacagttagg gctgggtaaa cgtcttgatc atcttccggc acagctttcc ggcggcgaac
    39781 agcaacgagt ggcgctggcg cgagccttta acggtcgacc tgatgtgctg tttgccgacg
    39841 aacccaccgg caaccttgac cgccagacgg gcgataaaat tgccgacctg ctgttttccc
    39901 tcaaccgtga gcatggcacc acgttgatta tggtgaccca cgacctgcaa ctggcggcac
    39961 gctgcgaccg ctgcttacgg ctggtgaacg ggcagttgca ggaggaagca tgattgcacg
    40021 ttggttctgg cgcgaatggc gctcgccgtc gctattaatt gtctggctgg cgctaagcct
    40081 ggcggtggcc tgcgtgctgg cgctgggcaa tatcagcgat cgcatggaga agggcttaag
    40141 ccagcaaagc cgggagttta tggcgggcga tcgggcgttg cgcagttcgc gcgaagtgcc
    40201 gcaagcgtgg ctggaggaag cgcaaaagcg cggcctgaaa gtcggcaagc agctgacttt
    40261 cgccaccatg acatttgcag gcgacacacc gcagctggcg aacgtcaaag cggtggatga
    40321 tatctatccg atgtatggcg atctgcaaac caatccccct ggcctgaaac cgcaggcggg
    40381 cagcgtattg ctggccccac gcctgatggc actgcttaac ctgaaaacgg gcgacaccat
    40441 cgacgtgggt gatgccacct tgcggattgc cggagaagtg attcaggaac cggattccgg
    40501 ttttaacccc ttccagatgg ctccgcgtct gatgatgaat ctggcggatg tcgataaaac
    40561 cggagccgtg cagccgggaa gtcgggtcac ctggcgctat aaattcggcg gcaacgagaa
    40621 ccagctcgac ggctatgaga aatggttgtt acctcagctt aaacccgaac aacgctggta
    40681 cggtctggaa caggacgaag gcgcgctggg gcgctcgatg gaacgctcgc aacagttcct
    40741 gctgctttcg gcgcttctga ccttgctgct ggcagtggca gcggtggcgg tggcgatgaa
    40801 tcattactgt cgcagtcgct acgatcttgt ggcgatcctc aaaacgctgg gggcagggcg
    40861 agcgcaactg cgtaagctaa tcgtcggtca gtggttgatg gtgctggcgc tttcagccgt
    40921 taccggcggg gcgataggcc tgttgttcga aaacgtgttg atggtgctgc tcaagcccgt
    40981 tctgcctgcc gcactaccgc cagccagcct ctggccgtgg ctatgggcgc tgggcaccat
    41041 gacggttatc tcgctgctgg tggggctacg accgtaccgc ttgttgctgg caacgcagcc
    41101 tttacgcgta ttacgtaatg atgtggtagc gaacgtctgg ccgctcaagt tttatctgcc
    41161 gattgtcagt gtggtggttg tgctgctgct cgccggatta atgggtggca gcatgctgct
    41221 ttgggcggtg ctggcgggcg cggtagtact ggctttgctg tgcggtgtgc tgggctggat
    41281 gctgctgaat gtacttcgcc gcatgacgct gaaatcgcta tctctgcgcc tggcggttag
    41341 ccgcctgtta cgtcagccgt ggtcaacgtt aagccagctt tcggcatttt cgctctcctt
    41401 tatgctgttg gcactgctgc tggtgctgcg tggcgatctg ctcgaccgct ggcagcagca
    41461 gctacctcca gaaagcccga actacttttt aattaacatc gccacagaac aggtagcacc
    41521 gctaaaagcg ttcctcgcgg aacatcagat agtcccggaa tcgttttatc cggtggtgcg
    41581 ggcgcggctg acggcgatta acgataagcc gacagaaggt aatgaagatg aggcgcttaa
    41641 ccgcgaactc aatcttacct ggcaaaatac gcggcccgat cataatccga ttatcgccgg
    41701 taactggccg ccaaaagccg atgaagtgtc gatggaagag gggctggcga aacgcttaaa
    41761 cgttggcctc ggcgatacag tcacctttat gggcgatacc caggagttcc gcgctaaagt
    41821 gaccagcctg cgcaaagtgg actgggaaag tctgcggcct aatttctatt ttattttccc
    41881 ggaaggggca ttagacgggc aaccgcagag ctggcttacc agtttccgct gggagaatgg
    41941 caatggcatg ttgacgcaac tcaaccgcca gttcccgacc attagcctgt tagacattgg
    42001 cgcgatttta aaacaggtcg gtcaggtgct ggagcaggta agtcgggcgc tggaggtgat
    42061 ggtggtgctg gtcactgcct gcggtatgtt gctgttgctg gcacaggtgc aggtgggaat
    42121 gcgtcagcgt catcaggagc tggtggtgtg gcgcacactc ggtgcgggga aaaaactgct
    42181 gcacaccacg ttgtggtgtg aattcgccat gctcgggttt gtttctggcc tggtggccgc
    42241 aattggtgcg gaaacggcgc tggcagtgtt gcaggcgaaa gtgtttgatt tcccgtggga
    42301 gccagactgg cgattgtgga ttgttctgcc gtgcagcggt gcgctgctgc tgtcgctttt
    42361 cggcggctgg ctgggtgcgc gactggttaa gggtaaggcg ctgttcaggc agtttgcggg
    42421 atgatgacag gtgttagatt gcctgatgcg ctacgcttat caggcctacg aagagcgtgc
    42481 aatatattga ttttgcaaga ttgtgtaggc cggataaggc gtttacgccg catctggcat
    42541 gaacagtatg cattttggca ataaatatga aaccaactgt
//
LOCUS       DAOTRK010000018        42523 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.018, whole genome shotgun sequence.
ACCESSION   DAOTRK010000018 DAOTRK010000000
VERSION     DAOTRK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.018"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            367..933
                     /gene="mntP"
                     /locus_tag="QIE41_001048"
     CDS             367..933
                     /gene="mntP"
                     /locus_tag="QIE41_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDS3529962.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(930..1739)
                     /gene="rlmA"
                     /locus_tag="QIE41_001049"
     CDS             complement(930..1739)
                     /gene="rlmA"
                     /locus_tag="QIE41_001049"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDS3529963.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLQERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1905..2114)
                     /gene="cspE"
                     /locus_tag="QIE41_001050"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1905..2114)
                     /gene="cspE"
                     /locus_tag="QIE41_001050"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDS3529964.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2127..2270)
                     /locus_tag="QIE41_001051"
     CDS             complement(2127..2270)
                     /locus_tag="QIE41_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDS3529965.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2940..3227)
                     /locus_tag="QIE41_001052"
     CDS             complement(2940..3227)
                     /locus_tag="QIE41_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDS3529966.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(3302..3445)
                     /gene="mgrB"
                     /locus_tag="QIE41_001053"
     CDS             complement(3302..3445)
                     /gene="mgrB"
                     /locus_tag="QIE41_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDS3529967.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3604..3843
                     /locus_tag="QIE41_001054"
     CDS             3604..3843
                     /locus_tag="QIE41_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3529968.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(3987..4778)
                     /gene="kdgR"
                     /locus_tag="QIE41_001055"
     CDS             complement(3987..4778)
                     /gene="kdgR"
                     /locus_tag="QIE41_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDS3529969.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTIISTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4955..6328
                     /locus_tag="QIE41_001056"
     CDS             4955..6328
                     /locus_tag="QIE41_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3529970.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLFLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            complement(6374..7255)
                     /gene="htpX"
                     /locus_tag="QIE41_001057"
     CDS             complement(6374..7255)
                     /gene="htpX"
                     /locus_tag="QIE41_001057"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDS3529971.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7447..9495)
                     /gene="prc"
                     /locus_tag="QIE41_001058"
     CDS             complement(7447..9495)
                     /gene="prc"
                     /locus_tag="QIE41_001058"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDS3529972.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9515..10213)
                     /gene="proQ"
                     /locus_tag="QIE41_001059"
     CDS             complement(9515..10213)
                     /gene="proQ"
                     /locus_tag="QIE41_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDS3529973.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10310..10807)
                     /gene="msrC"
                     /locus_tag="QIE41_001060"
     CDS             complement(10310..10807)
                     /gene="msrC"
                     /locus_tag="QIE41_001060"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDS3529974.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLSDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     TSNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10937..12220
                     /gene="yebS"
                     /locus_tag="QIE41_001061"
     CDS             10937..12220
                     /gene="yebS"
                     /locus_tag="QIE41_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDS3529975.1"
                     /translation="MALNTPQITPTKKITVRAMGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12189..14822
                     /gene="yebT"
                     /locus_tag="QIE41_001062"
     CDS             12189..14822
                     /gene="yebT"
                     /locus_tag="QIE41_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDS3529976.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            14902..16341
                     /gene="rsmF"
                     /locus_tag="QIE41_001063"
     CDS             14902..16341
                     /gene="rsmF"
                     /locus_tag="QIE41_001063"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDS3529977.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTASYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGIRNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCRWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTAQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            16459..16695
                     /locus_tag="QIE41_001064"
     CDS             16459..16695
                     /locus_tag="QIE41_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDS3529978.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16800..16991
                     /locus_tag="QIE41_001065"
     CDS             16800..16991
                     /locus_tag="QIE41_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDS3529979.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(16992..17648)
                     /gene="pphA"
                     /locus_tag="QIE41_001066"
     CDS             complement(16992..17648)
                     /gene="pphA"
                     /locus_tag="QIE41_001066"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDS3529980.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHIIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKVQGITGADHFWFGHTPLRHRVDIGNLHYIDTGVVFGGELTVVQLQ
                     "
     gene            complement(18044..18385)
                     /locus_tag="QIE41_001067"
     CDS             complement(18044..18385)
                     /locus_tag="QIE41_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDS3529981.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(18398..19270)
                     /gene="copD"
                     /locus_tag="QIE41_001068"
     CDS             complement(18398..19270)
                     /gene="copD"
                     /locus_tag="QIE41_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDS3529982.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLMFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(19274..19648)
                     /gene="yobA"
                     /locus_tag="QIE41_001069"
     CDS             complement(19274..19648)
                     /gene="yobA"
                     /locus_tag="QIE41_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDS3529983.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            19787..20017
                     /gene="holE"
                     /locus_tag="QIE41_001070"
     CDS             19787..20017
                     /gene="holE"
                     /locus_tag="QIE41_001070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDS3529984.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            20119..20775
                     /locus_tag="QIE41_001071"
     CDS             20119..20775
                     /locus_tag="QIE41_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDS3529985.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            20799..21461
                     /gene="exoX"
                     /locus_tag="QIE41_001072"
     CDS             20799..21461
                     /gene="exoX"
                     /locus_tag="QIE41_001072"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDS3529986.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(21458..23518)
                     /gene="ptrB"
                     /locus_tag="QIE41_001073"
     CDS             complement(21458..23518)
                     /gene="ptrB"
                     /locus_tag="QIE41_001073"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDS3529987.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(23675..24100)
                     /gene="tnpA"
                     /locus_tag="QIE41_001074"
     CDS             complement(23675..24100)
                     /gene="tnpA"
                     /locus_tag="QIE41_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEc46 family
                     transposase"
                     /protein_id="HDS3529988.1"
                     /translation="MSNHDDLLAGFLRKRHSVSKLVVHLIFTTKYRRKLFDSQMIAQL
                     REAFGSAAAKLECEIIEMDGEPDHVHLLVAYPPKLAVSVMVNNLKSVSSRLLRQQNAH
                     LRMQSKTGLLWSRSYFVCSTGEATIETLRAYVQSQSTPD"
     gene            24157..25326
                     /locus_tag="QIE41_001075"
     CDS             24157..25326
                     /locus_tag="QIE41_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS3529989.1"
                     /translation="MLRATKVRIYPTPEQAEFINAQFGAVRFAYNKALHIKKHSYQRH
                     RVNLNPRKDLKPLLAVAKKSRRYGWLKEYDSIALQQAVINLDVAFSNFFNPKLKAGKP
                     TFKSKHGRQSSYHCIGIKVLDAAIKIPKILPIEARLHREITGMLKSITLSRSATGKYY
                     ASLLCDDGMEAPAKPALISNVTGLDMGLAHFVIKSDGNKIASPRHLINASRNLRRKQK
                     ALSRKKKGSANRRKARIQLAAVHERVAGARADFQHKLSRAIVDENQAVIVETLKMANM
                     MKNHHLARAIGDAGWSGFITKLEYKATEKGVHLVKLDQWFASSKTCHCCGHKMSEMPL
                     HKRIWQCPECGVEHDRDINAALNIKRKGILELQAAGLVVSAHGGQRKSVTQTVAA"
     gene            complement(25490..26149)
                     /locus_tag="QIE41_001076"
     CDS             complement(25490..26149)
                     /locus_tag="QIE41_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDS3529990.1"
                     /translation="MANWLNQLQSLLGQSSSSASSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(26476..26832)
                     /locus_tag="QIE41_001077"
     CDS             complement(26476..26832)
                     /locus_tag="QIE41_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDS3529991.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(26899..27189)
                     /gene="yebG"
                     /locus_tag="QIE41_001078"
     CDS             complement(26899..27189)
                     /gene="yebG"
                     /locus_tag="QIE41_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDS3529992.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            27323..28501
                     /gene="purT"
                     /locus_tag="QIE41_001079"
     CDS             27323..28501
                     /gene="purT"
                     /locus_tag="QIE41_001079"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDS3529993.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFRKAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSEN
                     VTFDNVQNAVGAGLQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAAQIKVQA
                     "
     gene            complement(28556..29197)
                     /gene="kdgA"
                     /locus_tag="QIE41_001080"
                     /gene_synonym="eda"
     CDS             complement(28556..29197)
                     /gene="kdgA"
                     /locus_tag="QIE41_001080"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDS3529994.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(29234..31045)
                     /gene="edd"
                     /locus_tag="QIE41_001081"
     CDS             complement(29234..31045)
                     /gene="edd"
                     /locus_tag="QIE41_001081"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDS3529995.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(31280..32755)
                     /gene="zwf"
                     /locus_tag="QIE41_001082"
     CDS             complement(31280..32755)
                     /gene="zwf"
                     /locus_tag="QIE41_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDS3529996.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFF
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            33093..33962
                     /gene="hexR"
                     /locus_tag="QIE41_001083"
                     /gene_synonym="yebK"
     CDS             33093..33962
                     /gene="hexR"
                     /locus_tag="QIE41_001083"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDS3529997.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            34090..35532
                     /gene="pyk"
                     /locus_tag="QIE41_001084"
     CDS             34090..35532
                     /gene="pyk"
                     /locus_tag="QIE41_001084"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDS3529998.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(35664..36635)
                     /gene="lpxM"
                     /locus_tag="QIE41_001085"
                     /gene_synonym="msbB"
     CDS             complement(35664..36635)
                     /gene="lpxM"
                     /locus_tag="QIE41_001085"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDS3529999.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(36755..38077)
                     /gene="mepM"
                     /locus_tag="QIE41_001086"
     CDS             complement(36755..38077)
                     /gene="mepM"
                     /locus_tag="QIE41_001086"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDS3530000.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(38093..39025)
                     /gene="znuA"
                     /locus_tag="QIE41_001087"
     CDS             complement(38093..39025)
                     /gene="znuA"
                     /locus_tag="QIE41_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDS3530001.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTEKQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            39104..39859
                     /gene="znuC"
                     /locus_tag="QIE41_001088"
     CDS             39104..39859
                     /gene="znuC"
                     /locus_tag="QIE41_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDS3530002.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            39856..40641
                     /gene="znuB"
                     /locus_tag="QIE41_001089"
     CDS             39856..40641
                     /gene="znuB"
                     /locus_tag="QIE41_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDS3530003.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(40791..41801)
                     /gene="ruvB"
                     /locus_tag="QIE41_001090"
     CDS             complement(40791..41801)
                     /gene="ruvB"
                     /locus_tag="QIE41_001090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDS3530004.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(41810..42421)
                     /gene="ruvA"
                     /locus_tag="QIE41_001091"
     CDS             complement(41810..42421)
                     /gene="ruvA"
                     /locus_tag="QIE41_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDS3530005.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT        10362 a        10871 c        10423 g        10867 t
ORIGIN      
        1 tattacccct tcccttattt gttatttatt ttaacgtttc attgatatat aaatctaaat
       61 gaaaatcgtt atcaataaag caatgaaata atatattcca acagttgttt tatattctca
      121 aaatatgtta aggttgcgcc ctcatttggg gagtagccga tttccagatt ccggaaatgt
      181 acgtgtcaac atactcgttg caaaacgtgg cacgtacgga ctgaacactt tcagtcaggc
      241 gagaccatat gcacatcaat cgctatgcct gcatgaacgc gaaaaccatc gcggtttgtc
      301 gttcatgtat gcatgtttgc tgggggcgat gatgtgtttt atggataccc cggtcaggac
      361 gttgtcatga atatcactgc tactgttctt cttgcgtttg gtatgtcgat ggatgcattt
      421 gctgcatcaa tcggtaaagg tgccaccctc cataaaccga aattttctga agcgttgcga
      481 accggcctta tttttggtgc cgtcgaaacc ctgacgccgc tgatcggctg gggaatgggc
      541 atgttagcca gccggtttgt ccttgaatgg aatcactgga ttgcgtttgt gctgctgata
      601 ttcctcggcg ggcgaatgat tattgagggt tttcgtggcg cagatgatga agatgaagag
      661 ccgcgccgtc ggcacggttt ctggctactg gtaaccaccg cgattgccac cagcctggat
      721 gccatggctg tgggtgttgg tcttgctttc cttcaggtca acattatcgc gaccgcattg
      781 gccattggtt gtgcaacctt gattatgtcg acattaggga tgatggttgg tcgctttatc
      841 ggctcaatta ttgggaaaaa agcggaaatt ctcggcgggc tggtgctgat cggcatcggc
      901 gtccagatcc tctggacgca cttccacggt taataagaac gctgccagag gtgaatatta
      961 aagtccgtct ggcagtcgaa cacttctttt gctgccagtg tttgccagac ttctggcttc
     1021 gcacgccagg caaacggcgt catctgcaat aatgcgacgg cttcatcacc gcgaagacgc
     1081 atcggataac acaactcagc actctgctgt aatgtaaaac cttccagttg ttctgcatga
     1141 ggtgcatgaa gatgtacttc attgtaaatc agccccttca gctccatcaa atgtcgcggt
     1201 cccggcgtgg cagtaatgac ccaaccgcca ggcttcacta ctcgcgctaa ttcttctgct
     1261 ttacacggcg cgtaaatacg tattatggcg tccatactgg tatcggaaaa cggcaaacgg
     1321 tggctggaag caacacaaaa agtgacctgc ggatagcgtt tcgccgccgc ttttatcgct
     1381 accttcgaga catccaggcc aaacgtggtg atttcgggca acgcatcggc aaatgcgtgt
     1441 gtgtaatacc cttcaccaca gccaatatcc agcaccgccg tggccttttc atcaagccgt
     1501 tcctgcagtt gggcgacaat tgcatcacgc agtggctgat aatgtccggc atctaagaat
     1561 gcgcggcgtg cttgcatcat ttccgcgctg tcgcccggat cacgagaccg tttatgctga
     1621 acgggcagca gattgacata cccttctttc gccatatcaa actgatgtcg ctggggacat
     1681 atatagctgt ttttttcacg cgaaagaggc tgatggcaaa gtggacagga aaacgacatg
     1741 acaactccgg caggatacta aaggccgcaa gtgtaacgcg aatcacgcgg cgggcaaata
     1801 tccgaacgac tggagcgaag ataaagcaaa gtaaaaagcc tcgcattcga cgaggcttta
     1861 tagcgagatt gaagcgtatt cacacttcag atcagtggat tcgatcagat agctgttacg
     1921 ttaacagctg ccggaccttt ctgaccgtcc tgaatttcga actcaacgtt ctgaccttca
     1981 gccagagttt tgaaaccatt accctggata gcagagaagt gtacgaacac gtctttgctg
     2041 ccatcagccg gagtaatgaa gccaaaacct ttagactcgt tgaaccactt aacctgacct
     2101 ttaatctttg ccatttgaaa aattccttag attgttttct tcgcccgcag gcataacata
     2161 gataaaactg acacattact gcatgaggca ccaatataag gctcggcaga gaagcggtat
     2221 tcaacgtcaa cgtgtttact caggacttct ttactgaaaa tgccacacat aaacagaact
     2281 gtacctcgtt taacccgaaa tctgttatca catacaacgt taattatggc aagccatttt
     2341 taaacatgtc tcgatcagac gcacaaatct cgatacgtct tccacttttt ttgcacactt
     2401 atgcaacgga atacgcgccg atatatcatt gtgcttaacc ttgccagttc aggcagatac
     2461 ttaaactgac gtattttcta acatagttca ataagttcag ttgttccagg ccaatgggag
     2521 aagttattac atagtgcgtg caatatcaca ttttctgcta tgcaatgaat aaaaagttat
     2581 atcacttttt ctcataaaac agtcagttaa cggctatcaa tcaccccaaa gagaagtcaa
     2641 tcgccaaagg tattgtaaat ttaaaataag aaaaattgat gaatgcgcaa aaaaatcaag
     2701 agagaaacgt ttcgctaata aataatacca cacttaatca caaatccgat gtaaacatcc
     2761 taataaatta atgggctaaa tatctttttc tgatatcccg ttacgcaaat aaataatatg
     2821 ataattattt gaagtcagac cagagtttta atttgaagca gttgcaccat ggcagtgcgt
     2881 ataatcagga tgaattgagt aacccttcaa cgacggggtt actcaagatt gaggaaggat
     2941 tatcgttcgg cgaccagacg aataatatct tcgtcttcaa ccgatttttg actctcaacg
     3001 gtctttttat ctgctttctc aggttcgttt gcttcgcaca aacgacgtaa cagagcattt
     3061 tgacgttttt gctgatccag caacgcctca agcagttcaa tctgttcgtt agtccgtgaa
     3121 ctggcacgat tgataaaaaa ccacaagata agcccgataa aaagaaccac caccgataca
     3181 accaaagacg caatgttcat cacgcctgaa ttaacaactt cgttcatttc accacctctg
     3241 agtagaggcg ctattctacc actgctggag aggaagaaaa tctagtgctg aaaaaatgat
     3301 atcaccacgg gataaactgg ttaatggcac aaattccgct gaaaaattgt acatcctgat
     3361 cgcacatcat gttgaatacc tgcgcccaaa gcagcaagca agccaacacc acgacaacca
     3421 gaacgaccca tcgaaacttt ttcactccaa tctccgtttc acctacctta tgtcattaaa
     3481 ctagcatgcg ttagttaaac agcgcctaac tatgtcgata ttcgtgcttt ttcagaatga
     3541 gtcgcttgct tcagcgcata ttgccgctac gattaagcga acataaaaag agaagaggtt
     3601 gtaatgcgat tcatcattcg cacagttatg ctgatagcac tggtatggat aggattatta
     3661 ctcagcggat atggcgtttt gattggaagc aaagagaatg ccgcgggatt aggtttgcaa
     3721 tgtacctatc tgaccgccag aggcaccagc acggtacaat acctgcacac taaaagtggt
     3781 tttctgggga taacagattg cccgttgtta cgtaaaagca atattgtggt cgataatggt
     3841 taagggtaat aaataaccag attctcactc atgagtaaaa tattcttatc gcaagtgcgt
     3901 ttaatataac gccaccttca actttatttt tctcatgcta aataaagaac ctgtctgttt
     3961 gatattcaga caggttctta ctctcatcag aatggatagt cgtgataacc catttgggca
     4021 gaaattttgc gcgccgcggt atgcaacatt gcgacatatt cctgtaaacg ctcttcagag
     4081 aaacgcaacg tcgggaagga gatgctaaga ccggcaatga ccacgccaaa gcgatcaaat
     4141 accggaaccg caatgcaacg cagtccttct tcctgctctt cgttatcttc gccatacccc
     4201 tgctcgcgca cctggtccag aacgggtaat aacgcttctg tactgatgat ggtccgttcg
     4261 gtactgcgtt tatactccac gccctcaaga atttgcttca cttcatcgcg atcgcgccat
     4321 gccagcagta ccttaccaat cgcggtgctg tacagcggat tacgacgccc aatccgtgaa
     4381 tacatgcgca aattgtacat agagtcaatt ttgtgaatgt aaacaatact gtcttcgtcc
     4441 agtgcgccga ggtggatagt ttctttggtc aggcgggaga tctcacgcat ctggatatct
     4501 gcgctacgaa ttaaatcgac gttttgtaac gcgcgagcgc ccagttcaaa caatttcagg
     4561 gtcagggaat atttctccga ctccccttcc tgcgccacat aacccagggt tttcatggtc
     4621 tgtaaaaagc gataaacggt gctttttgac atcatgacgc gctgcgacag ctcggttatc
     4681 cctatttcgc gctcttcacc cagcgcctgc aaaatgccaa aaacttttag cacggaagat
     4741 acagaatcag gctgtttatc cagatctgcg ttagccattt atcacctcat tgcgagtgtt
     4801 ttataaaaat cagaactgtt ttttattata atttcgcacc tgggtggtcg caatccatct
     4861 tttgccggtt agttacaatt ctgcgacatc caccgtgaat atcagtgcta gaatcttacc
     4921 cctgttgatt attcaccaaa gatataaaat tcctatgcca aaagttcagg ccgacggcct
     4981 gccgttgccc cagcgatacg gtgcgatatt aaccattgtg attggtattt cgatggctgt
     5041 ccttgacggc gcaatcgcca acgtcgcctt gccaacaatc gccacggacc ttcatgccac
     5101 accggccagt tccatctggg tagtgaacgc ctatcaaatc gccattgtca tctccctgct
     5161 ctcattttcg tttctgggcg atatgtttgg ctatcgacgt atttataaat gcggtctggt
     5221 cgtttttctg ttgtcttcac tgttctgcgc cctttctgat tcgctgcaaa tgctcaccct
     5281 tgcgcgtgtc atacaaggtt tcggcggtgc agcgttgatg agcgttaata ccgcacttat
     5341 ccgcctgatc tatccacaac gttttctggg tcgagggatg ggcataaact cgtttattgt
     5401 tgccgtttct tctgctgccg ggccgacaat tgctgcagca atcctctcca tcgcatcctg
     5461 gaaatggtta tttttaatca acgtaccgtt gggtattatc gccctgcttc tggcgatgcg
     5521 ttttctgcca cccaatggtt ctcgcgccag taaaccccgt ttcgacctgc ccagcgccgt
     5581 gatgaacgcg ttaaccttcg gcctgcttat cactgcgttg agtggtttcg ctcaggggca
     5641 atcgctgaca ttgattggtg cggaactggt ggtaatggtt gtcgttggta ttttctttat
     5701 tcgccgccag ctttttcttc ccgtaccgct gctaccggtg gatttactgc gtatcccgct
     5761 gttttcactt tctatttgca catctgtttg ctctttctgc gcacaaatgc tggcaatggt
     5821 ttccctgccc ttttacctgc aaaccgtgct cgggcgtagt gaagtcgaaa caggtttact
     5881 tctgacaccg tggccgttag caacgatggt gatggctccg ctggcaggct atttgattga
     5941 acgcgtacat gcaggattgc tgggtgcttt aggattgttc atcatggctg cggggctttt
     6001 ttccctggtt ctgctgcccg cgtcacctgc ggatatcaat attatctggc cgatgatctt
     6061 atgtggcgct ggatttggct tgttccagtc acccaataac cacaccatta ttacctccgc
     6121 tcctcgcgaa cgtagcggtg gagccagtgg catgttagga acggctcgtc tactgggtca
     6181 gagtagcggc gcggcgctgg tggcgctgat gctaaatcag tttggtgata atggtactca
     6241 cgtctcgctg atggctgcgg ctattctggc agtgattgct gcctgtatca gtgggttacg
     6301 tatcactcag ccaagatcca gggcataaaa aaagcgcgtc gagcaggacg cgctttttag
     6361 tatttacttc atattacttc aggtattcac ccgtacgcag agcttcaata cgtttatcca
     6421 gcggcgggtg agtcatgaac aactcactga gcgatttcga cttaccgtta atacagaaag
     6481 ccatcatgct ggttgcttct tgcggttcat agctggtttt caggcgctgc agcgcggcaa
     6541 tcattttctc gcgaccaacc agttttgccg aaccggcatc agcgtggaat tcacgatgac
     6601 gcgagaacca catggtgata atgctcgcca gaataccaaa caccagttcc agaaccgttg
     6661 caaccgcaaa gtagatcagc gggttgccgt tgctctcttc accttcatca cgatttccgc
     6721 ccataaaacc cgcggcaagc tgcgccagaa tacgggaaat aaagataacg aaggtgttca
     6781 ccacgccctg aatcagcgtc atggtgacca tatcaccatt ggcgatgtgg ctgatttcgt
     6841 gagcaattac cgcctcggct tcatccgggc tcatgttctg cagcaaaccg gtgctgacag
     6901 caaccagaga ggcatcacgg cgcgcaccgg ttgcaaaagc gttgatgtcc ggcgcatggt
     6961 agatagccac ttgcggcata gcgatccccg cctgacgagc ctgggttgct acagtattga
     7021 ccagccaacg ttccctttcg ttacgcggtt gctcgatcac ttccccgcca acagatcgta
     7081 atgccatcca tttggacatc agaagcgaaa cgaaggaacc accaaaaccg aacagcaagg
     7141 ccatgatcat cagcccctga acgctgctcg actgtatccc tgtcaggctc agtaccagcc
     7201 cgaaaacgac cattacggcc aggttcgtta gcaggaagag cgcgattcgc atcataattt
     7261 tctttttacc tcagtttaac aaaacgcaat atgcgatacc cacatcgtat gggttacgcg
     7321 actattttca agtctggata gtgcgtaagt caccagaaag acacaacttt acattttgta
     7381 gcatctgatt tacggcatct tgccgctgtt aaaaaatcag gcacaatttc ttgtgcctga
     7441 ttgatattac ttgacgggag cgggttgttc cgcgggtctg gctttttcaa gcttcgccag
     7501 atcgagtgcg atattcaccg tctcatccag ataaggatcc ggctcctggt aatctttcgg
     7561 tagatcatcc agtttcttca actccggttt accttcgcgc ttaaagcgtt cgttcaaacg
     7621 cgccagacgc gtcgcatcat cttcattatt ctctttctca cgcaccgcgt aattcagaga
     7681 aacgatattg cgcttgtcct tcatagcgtt gaagcgcgcg atatccttca tgatgttctg
     7741 gaactctgga tctttcgcga tacgcgcatt atgttccttc agcagctccg gtccaaaggc
     7801 cgttaaatcg cctgatttca cataggtcgc ggcatcgatg ctatcccacg gcagcgcgtt
     7861 atcttcgaat ttctcaccgg tttccgtttc ttcattaccc gtcggcatga tgatgtctgg
     7921 cgttacgcct ttacgttgcg tactgccgcc gttaacgcga tagaatttct ggatcgtgta
     7981 ctgcacagaa cccagcgctg gccattcagg acgtaacatc tgatcgtaga tacggttcaa
     8041 tgaacggtat tgctgaacgg tgcctttacc aaacgtcggt tcacccacaa ccagcgcacg
     8101 accgtaatcc tgcattgccg cggcaaaaat ttctgaagcc gaagcactga aacggtcaac
     8161 cagtaccacc agcgggcctt tatagaagac ctgtccgtcg gtatcgctgt cttcacgaac
     8221 tttaccgttg ttatcacgga cctgaacaat cgggccggat ggaataaaca gaccggagag
     8281 cgataccgct tcggtcagcg ccccaccacc attgctacgc aggtcgatga tgacgctgct
     8341 gacattctgt ttttccagtt tctgcagttg cactttgaca tcgtctgtca accccacata
     8401 gaagcccgga atatccagca cgccgacttt ctctttaccg acggtcttca ccgacatttt
     8461 aaccgcacgg tcttcgaggc gaatacgttc acgggtcaac gttacagtac gggtcttggt
     8521 ccctttacca gcaggtaaaa tttccagacg aactttactg cccttcggcc ctttaattaa
     8581 ggcaaccaca tcatcaagac gccagccaat cacgtcaacc atcggcttgc ctgtttgacc
     8641 aacaccgaca attttgtcac caacgctgat agctttactc ttcgctgccg gaccacctgc
     8701 caccatcgaa ttgataacgg tgtagtcatc atccatttgc agaactgcgc caataccttc
     8761 cagcgacaaa ctcatttcag tgttgaactg ttcggtatta cgcggggaaa gatagttggt
     8821 atgcgggtcg atttcacgcg caaacgccgt cattgccagc gagaaaacat cttcgctgtt
     8881 ggtttgcgcc agacgacgaa tggcaaattt gtagcggcga gtcagggttt cacgaatttc
     8941 tttatccgtt tttcctgcca gcttcaggct taactcgtcg aatttgactt tactgtccca
     9001 cagcgcgttc aactcagcct cgtttttcgg ccatggcgct ttgctgcggt caaggttata
     9061 agtgtcgttg ccggtaaaat ccatcggctt ttccagtacc gacaaagcgt actggtaacg
     9121 ctcaaaacgg cgcttttgcg ccagattgta gagatcgtag aaaacgtcga gttttcctga
     9181 acgcagttca tcacctaact cggttttctt tttcgcgaac tgttcaacat cgcttgccag
     9241 cagcacgttg tggctgtaat cgagcagatt caggtagcgg tcaaagattt tggccgaaaa
     9301 tgcctgatcg aggtcgaact ggcgataatg agaacgggtg aagcgcgacg ttacgcgctc
     9361 acttaccgtc gcatgctgcg tctcttcctt taataccgga atttgatcag cacgcgtgat
     9421 atcttctaca gcgaaggtct ggcctgctat tgcaagcagg ccagctaacg cggtaagcct
     9481 aaaaaacatg ttcatgcctg gcccggcctc cgtttcagaa caccaggtgt tctgcgcgca
     9541 caatcaaaga catacccgaa ttcagctgga cgcggacgcc gtctttggtg atttctaata
     9601 cggtggcatc catcgcgttt tgacccgctt tcaccttcag ggcttgtccg acagtcagag
     9661 ctgaaatgtc agaaaccggg gtgtgctgtt cttcgcgagg tgcttttact gtttttggcg
     9721 ctttctctac cggcttttgc gcacgaggtt tacgttcagc gccttctttg cggcgtggcg
     9781 tggtcggacg tggcttgcgt tcgcggcgcg gtgcgtcttc tttctcacca gcagctgcgg
     9841 cagcttcgcg ttttttcgct tgctgttcag cacgctgtgc ctgaacacgc gctttcgctt
     9901 cttcaagctg cttgcgagca tgctctacat gttgctcgtc cagctcaccg catgggttgc
     9961 cgtcaagatc gacacgcgtt gcgccgggtt taacaccgta aaggtaacgc cagctcgaag
    10021 tgtagagacg taaagcggat cgcaattgcg ttttgctcag gttcatttcc ccggcaacac
    10081 gatcgaccaa atcctgaaaa ataccgattt tcagcggacg cgcttcacct tccgcactga
    10141 aacagtgggg aaaacgttcg gccagaaacg cgattacttc tttactgcta ttcaacttag
    10201 gttgattttc catgaaattt cctgattaca acggacgtag ccaacaagcg caggcatgaa
    10261 caggcgtcat tataatgacg ctatcagtaa atgctacgtt atccgttgat tatcctgcga
    10321 cgctcgcaaa gaattttttg taatccgtcg ttgcaagcac cttttcaagc tgtgccacaa
    10381 gctgacgtaa gccttgctcg tcctcgtctg taaagcgacc gaagacggta ctatcgatgt
    10441 cgagaacacc aataatctga tttttcacca ccagcggcag aacaatttca gaattactcg
    10501 tcgcatcaca ggcaatatgc ccgtcaaacg catgaacatc ctcgatacgc tgcacttgat
    10561 tgcgggcaac cgcagtaccg cacacgccgc gcccgacggg tatccggaca caggcaattt
    10621 tgccctgaaa tggtccgagc accagtgtat cgtcctcaag caaataaaaa cctgcccagt
    10681 ttacgtcact gagacgctca tataacaacg cactggtgtt agcaagcgtt gccagaaaac
    10741 tggtttctcc tgccatcagc gcgttaaagt cgcgatttaa atccgcgtaa aattctgttt
    10801 tgttcattat ataatcactt ggttgtctta cctggaactg ccagcctatt aaaataagca
    10861 ttaaatgcgt taatgctcaa gatcattccc atcatgggtt aagattaatg ttaattctta
    10921 ttacatttgg cacgtcatgg ctcttaacac accacaaatt acgccgacaa aaaagataac
    10981 agtgagggca atgggcgagg aactgccgcg tggtgattac caacgttgcc cgcaatgtga
    11041 catgctgttt agcctgcctg agataaattc tcatcaaagt gcctattgtc cgcgctgcca
    11101 ggcaaaaatt cgtgacgggc gcgactggtc gctaacgcgc ctggcggcaa tggccttcac
    11161 catgctgttg ctgatgccgt ttgcctgggg cgaaccgctg ttgcatatct ggctgttagg
    11221 cattcgtatt gacgccaacg tcatgcaagg catctggcaa atgaccaaac agggcgatgc
    11281 gataacggga tcaatggtct ttttttgcgt tattggtgcc cccctcattc tggtgacctc
    11341 catagcttat ttatggtttg gtaaccgact gggaatgaat ctacgtccgg tactgctgat
    11401 gcttgagcga ctgaaagagt gggtaatgct cgatatctac ctggtcggca ttggcgttgc
    11461 ttctataaag gtgcaggatt atgcccatat ccaggcgggt gtcggcttgt tctcttttgt
    11521 ggcgttggtg attttaacga cggtgacgtt gtcacatctc aatgtcgaag aactgtggga
    11581 gcgattttat ccgcagcgcc ccgctacgcg tagggacgag aaactccgtg tctgtcttgg
    11641 gtgccatttt accggctatc ctgatcagcg tggtcgctgc ccgcgttgcc atatcccgct
    11701 acgcctgcgt cgccgtcata gtctgcaaaa atgctgggcg gcgctgttag cgtcaatcgt
    11761 tttgttgtta cctgccaacc tgttgcctat ttctatcatt tatctgaacg gaggacggca
    11821 ggaagataca attctttccg gaattatgtc gctggcaagt agcaacattg ccgttgcggg
    11881 aatcgtgttt atcgccagta ttctggtacc gtttactaaa gtgatcgtca tgttcacttt
    11941 actgttgagt attcatttta aatgccagca aggtttacgc acacgcattc tgttactgcg
    12001 gatggtgacc tggattggtc gctggtcgat gctcgacctg tttgtcatat ctttaaccat
    12061 gtcgctgatt aatcgcgatc agatcctcgc ttttactatg ggaccggctg cgttttattt
    12121 cggcgcagcg gtaattttga ctattcttgc tgtggaatgg ctggacagcc gcttactttg
    12181 ggatgcacat gagtcaggaa acgcccgctt cgacgactga agcgcagatt aaaaataaac
    12241 gccgtatctc acctttctgg ctgctgccgt tcatcgcgct aatgattgct ggttggctga
    12301 tttgggacag ttatcaggac cggggtaata ccgtcaccat cgactttatg tcggcggatg
    12361 gtattgtccc gggccgcacc cctgttcgtt atcagggcgt tgaagtcgga actgtgcagg
    12421 atatcagcct gagcgacgat cttcgtaaga ttgaagtcaa ggtcagcatc aagtctgata
    12481 tgaaagatgc gctgcgcgaa gagacacagt tctggctggt gacgccaaag gcctcgctgg
    12541 caggtgtctc cgggctggat gccctcgtcg gtggtaacta tatcggcatg atgccaggta
    12601 aaggtaaaga gcaggatcac tttgtcgcac tcgataccca accgaagtat cggctggata
    12661 atggcgatct gatgatccac ctgcaagccc ccgatctcgg ttcgctgaac agtggttcat
    12721 tggtctattt ccgcaagatc ccggtgggta aagtctacga ctatgccatc aatcccaata
    12781 agcaaggcgt ggtgattgat gtcctgatcg agcggcgttt taccgatctg gtgaaaaaag
    12841 gtagccgttt ctggaacgtt tccggcgttg atgccaacgt cagtatcagt ggcgcgaagg
    12901 tgaaactgga aagtctggcg gcactggtta acggtgcgat tgccttcgat tcacctgaag
    12961 agtcgaaacc tgccgaggcg gaagatacct ttggtctgta tgaagatctc gcccacagcc
    13021 agcgtggcgt aataataaaa ctggaactgc cgagtggggc cggattaacc gccgactcga
    13081 cgccgttaat gtatcagggg ctggaagtcg gacagctgac taaactggat ttaaatcctg
    13141 gtggtaaagt cactggggaa atgaccgttg atcccagcgt cgtcaccctg cttcgtgaaa
    13201 atacccgcat cgaattacgt aatccgaaat tatcccttag cgatgccaat ctcagcgccc
    13261 tgctgaccgg aaaaaccttt gagttggtgc ccggcgatgg cgagccacgc aaagagttcg
    13321 ttgttgtgcc aggtgaaaaa gccctgctac aagagccgga tgttctgaca ctgaccctga
    13381 cagcaccaga aagttacggc attgatgctg gtcagccgct cattcttcac ggcgtgcagg
    13441 taggccaggt tattgatcgt aaactcacca gcaaaggcgt cacctttacc gtcgccatcg
    13501 agcctcagca tcgcgaactg gtaaaaggcg atagcaaatt tgtcgtcaac agccgtgtcg
    13561 acgtgaaggt ggggctggat ggcgttgagt ttcttggtgc cagcgcctca gaatggatta
    13621 acggcgggat acgtattctg ccaggcgata aaggcgagat gaaagccagc tatccactgt
    13681 atgccaatct ggaaaaagcg ctggagaaca gccttagcga tttacccacc acaaccgtga
    13741 gtttgagtgc agagacgctg ccggatgtgc aggcaggatc ggtagtgctg taccgtaaat
    13801 ttgaagttgg tgaagttatt accgtgcgtc cgcgagctaa cgcgtttgat atcgatctgc
    13861 atattaagcc ggagtatcgc aaccttctga ccagcaacag cgtgttctgg gcagaaggtg
    13921 gggcgaaagt tcagctgaat ggtagtggtc tgactgtaca ggcatccccg ctctccagag
    13981 cattaaaggg ggctattagc ttcgataacc tcagcggtgc cagcgccagt cagcgtaaag
    14041 gcgacaagcg tattctgtat gcttccgaaa cagcggcccg tgcggttggc gggcagatta
    14101 cgcttcacgc tttcgatgcc ggaaaactgg cggtcgggat gccaattcgc tatctcggta
    14161 ttgatatcgg gcaaatccag acgctggatc tgattaccgc gcgcaatgaa gtacaggcaa
    14221 aggcggtgct ctatccggaa tatgttcaga cctttgctcg cggtggtaca cgcttctcag
    14281 tggtcacacc gcaaatttcg gcagctggcg ttgagcatct tgatactatc ctccagccgt
    14341 atatcaacgt cgaaccaggc cggggcaatc ctcgccgcga ctttgaatta caagaggcca
    14401 ccataactga ttcgcgttac ctggatggct taagcattat tgttgaagcg ccggaagccg
    14461 gttcgttagg catcggtacg cctgtgctgt tccgtggtct ggaagtcggt acggttaccg
    14521 gaatgacgct ggggacattg tcagatcgcg tgatgattgc gatgcgcatc agtaaacgct
    14581 atcaacacct ggtgcgtaac aattccgtct tctggttggc atcgggttac agtctggact
    14641 ttggtctgac gggcggcgta gtgaaaaccg gcacctttaa ccagtttatc cgtggcggca
    14701 tcgccttcgc cacgcctcca ggtacgccac tggcaccgaa agcccaggaa ggcaagcact
    14761 tcctgttgca ggaaagtgaa ccgaaagagt ggcgtgaatg gggtactgcg cttcccaaat
    14821 aatgcccact gctccggcgt gcctgcgccg gagcgtttat gctaaactgc gcgcctgttt
    14881 ttttgccagt ggtatatgct cgtggcccaa cacaccgttt atttcccgga cgcctttctg
    14941 acgcaaatgc gcgaagcgat gccttcgacg ctctcttttg atgattttct tgccgcctgt
    15001 cagcgcccgt tgcgccgcag cattcgcgtt aatacgctga aaatctccgt tgctgatttc
    15061 ctgcaattaa ccgcctctta tggctggacg cttacgccaa ttccgtggtg tgaagaaggt
    15121 ttctggattg aacgcgacga tgaagatgca ttgccattgg gtagtaccgc cgagcattta
    15181 agcggcctgt tttatattca ggaagccagt tcaatgttgc ccgttgccgc cttgtttgct
    15241 gacggtaatg caccacagcg ggtgatggat gtcgctgctg cgccaggctc caaaacgacg
    15301 caaattgccg cgcggatgaa taacgaaggg gctatccttg ccaatgagtt ttccgccagt
    15361 cgggtaaaag tgttacatgc caatatcagc cgctgtggca tccgtaatgt tgcgctcacg
    15421 cattttgatg gccgtgtatt tggtgcggca gtgccagaaa tgttcgatgc cattttgctg
    15481 gacgctccct gctccggcga aggcgtggtg cgtaaagatc ccgatgcgct aaaaaactgg
    15541 tcaccagaaa gcaatcagga aatcgcagct acccaacggg aactgatcga cagcgccttt
    15601 catgcattac gccctggcgg tacgctggtt tactcgacct gtaccttaaa ccaggaagaa
    15661 aatgaagccg tctgccggtg gcttaaagag acttaccccg atgcagtaga gtttttaccg
    15721 cttggcgatc tcttccctgg tgcaaataag gcgctgaccg aagaaggctt tttgcatgtt
    15781 ttcccacaaa tttacgactg cgaaggcttc ttcgttgctc gtctgcgtaa aactcaggcg
    15841 atccccgtct tacccgcccc aaaatacaaa gtgggcaatt tcccgtttag cccggtgaaa
    15901 gatcgcgaag ctggacaaat tcgtcaggcg gctgcaggtg ttggcttaaa ctgggatgaa
    15961 aacctgcgcc tctggcagcg tgacaaagaa ctgtggttgt tcccggtggg cattgaagcc
    16021 ctgatcggta aagtccgatt ttctcgcctg gggattaaac ttgccgaaac gcataacaaa
    16081 ggttatcgct ggcagcatga agcggttatt gcccttgcct cacccgacaa tgtgaacgct
    16141 tttgaactaa cggcgcagga agcggaggag tggtatcgcg ggcgcgatgt ttacccgcaa
    16201 gccgcgccag tagcggatga tgtattggtt actttccagc atcagccgat tggtttagcc
    16261 aaacgaattg gttcgcgact gaaaaacagc tacccgcgtg aactggtgcg cgatgggaaa
    16321 ctttttaccg gtaacgcctg acagcgcaca aaaaaagcgc actttttgac tggcacattc
    16381 ggctgcctca actaggctga aaaatggtgc gatcggactg gtcgtaccac aatcggcagc
    16441 taaatggaga gcacgaagat gaaaaccagt gtgcgcatag gcgcttttga aatcgacgac
    16501 ggcgaattac acggcgaatc gccgggtgat cgaacgttaa ccattccttg taaatctgac
    16561 cccgatttat gtatgcaact ggatgcctgg gatgctgaaa ccagtatccc tgccctgcta
    16621 aatggcgaac actctgtcct ttaccgtacc cgttacgatc aacaatctga tgcctggatt
    16681 atgcgtcttg cctaatccaa aaagaacccg tcggcatggc gggttatttg tcctggttat
    16741 tcccccgttg taaaatctct cctaaactta acggtacggc accacacttc ggggatgaaa
    16801 tgttcgcgct ggtacttttt gtttgctacc tggatggcgg ttgtgaagat attgttgtgg
    16861 atgtctacaa cacggaacag cagtgtcttt attctatgag cgatcaacgg atccgccatg
    16921 gcggttgttt tccgattgag gattttatag atggtttctg gcgacctgca caggagtacg
    16981 gtgattttta attattgcaa ttgcacaaca gtcagttcgc ccccaaagac aacacctgta
    17041 tcaatataat gcaggttgcc aatatccacg cgatggcgca acggtgtatg accaaaccag
    17101 aaatgatcag cacctgtaat tccctgcact ttttggcgtt cacttaatcg cgagcggctc
    17161 cacaagacct gatgcaaatc gacgtccttt tgccattcat aaacatcatc tggataatcg
    17221 gcatgagcaa taatatgctt gccggtgcga ctgtgtactt caagaataaa gggcaaatgc
    17281 tgacattttt ccagcgccgt tttcgcttgt ttctgttgat tatctgccag cgcaataaac
    17341 cagtcgccgc cattcatcaa ccataaagac atctgctggg atgccagcgc atccatcgcc
    17401 atctgttcat gattgcctct taccgcacga acccaatgtt gttccagtaa ctgcaggcaa
    17461 cgtaaacttt gcggcccacg atcgataacg tctcccactg agataagtaa atctcgccac
    17521 ggatcaaaac cacaatgcca taatttgcgg cgcaactgct caagacaacc gtgtatatcg
    17581 cctgaaagcc agatatgtcg ccattgatga cccgcaattc tctgataaac gggcgcaggc
    17641 tgtttcatca atattttcct cccgcgctaa agatcacata atcttaacaa gaatgttaaa
    17701 aaacgctgga ctcagacagt agagtgtgtg ttatggttga ctataaagtc agcgaaggga
    17761 atgcttctgg cttttaacag ataaaaagag accgaacacg attcctgtat tcggtccagg
    17821 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
    17881 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
    17941 taaaaagcgc ggtggccatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
    18001 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
    18061 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
    18121 gaatcaccga aatgggttgg gctttagtct tcccactggc aacttccttt tgtgcgatat
    18181 cgtttaaagg atactgcacg agggtacttg ggttgatgac atacaaagca ttacccggtc
    18241 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
    18301 ggctaacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
    18361 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
    18421 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
    18481 caaaccaggg tgccgcacga tcttcatcct gcctcatgcg cggtacaaga acataacgat
    18541 tagccagcgc gatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
    18601 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
    18661 ttgccagtac gccgatcacc gcaaaatgcc cgcaccagga aaaacgcatc agcgcctgaa
    18721 tagcctggtg tcgccaacga cctttgatga gttgcataca ccagagcacc ggcagtaatc
    18781 ccccaaacca cgccgccgca cagatcaggt gaatcgcgtg attagtctga tggattttcg
    18841 ccgttacccc ttcattcagc gtcgcatgcc cgactcccgc cagcagaata aattgcgcgg
    18901 tagtgagcat aaacatcaaa cgtggcatat tccgcggttg cataagggcg acgatcagcg
    18961 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
    19021 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
    19081 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
    19141 gaaaacgctt cgtcagcaag cgacgaatcg ttaagggggc cagccaggca ccgtacattg
    19201 cgaagccaaa gaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
    19261 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
    19321 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
    19381 caatcagttg cttttggtcc tgctcattcc gcttcgccgg taatgtttta atattttcgt
    19441 tttttggccc cgtgattttt gcaccactga atcctgtttc aacgccttcc gagaagttta
    19501 aagtgattgc ctgcggggca gctgtcactt gcgcatttgc cgcaggatac tgatgcgtta
    19561 aatgcgcatg tgcccagacg gaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
    19621 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
    19681 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatccag
    19741 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
    19801 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
    19861 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
    19921 tgaacatttg cgcagctggt ttcgcgagcg gcttatcgcc caccgtttgg cttcggtcaa
    19981 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacagag ttacactttc
    20041 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
    20101 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
    20161 aaaacctcac tgaccgaaca ggtagctcat catctggagt ttgtgagggc cgccgccagg
    20221 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
    20281 cgtgcccttc ctgccccacc cgatctctcg ctgttagacc cgctttgtta tgctgcaacg
    20341 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
    20401 ggaattgcgg tgttcgcacc gtggcgaaaa acaccaggga tctaccatca gagtcatggt
    20461 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
    20521 gatatggatc cgacctgttc actgtttacg acagggcaat gtctggggga acccgaccta
    20581 ctggcatcta cccgccgctt acaatttttt tcacatcagt actcaattgc cgtgctgatg
    20641 gcaaatgccc gtggtaacag tgcactatgg gatgaacatg gtcgtctcat cgttcgcgcc
    20701 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
    20761 attccattac gctaggcttt ttcggcctgg agcatgccat gttgcgcatt atcgatacag
    20821 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
    20881 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaggcga
    20941 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
    21001 tcccgcacta ttacggtagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
    21061 tactgcctga gatgcccggt gagtggattt gcactatgaa actggcccgt cgtttgtggc
    21121 cagggatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
    21181 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgct gcgttgctta
    21241 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
    21301 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
    21361 ccgaacgcga tccgggctat ctgcgctggt tgtttaataa cctggacagc atgagcccgg
    21421 agctgcgttt aacactgaaa cattatctgg aaaatactta atcctcaggc gtagcgggta
    21481 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
    21541 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
    21601 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
    21661 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
    21721 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc ggattccccc
    21781 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcattgtt gttacaacat
    21841 caacaaacgg tacctgggcg ataacgccgt ggaacaattc cgggcgttga ttaattgcaa
    21901 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acagagcgaa ggagagccat
    21961 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
    22021 tcaggaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
    22081 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
    22141 caatacttgc gccgtaagaa ccatagccat acaccagcaa tgggttgtgt cctttgcgaa
    22201 aatgtttgcg atgatagacc agcgaaaccg gaacttcgac gccatcacgg gcgactatcc
    22261 acaggtgttc actgcggtaa ttcgccgcat caaaaccggg cacttctgtt tgttttaata
    22321 cacgacgctc accggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
    22381 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
    22441 aggccggatc atcaaaggca atgccaatga cttcccgggt cttgcggttg atttggcgca
    22501 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
    22561 cttccagcat aatgttatcg cgtggcggaa ttaactcttc ccactgttgc tcatcacgca
    22621 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaccgat
    22681 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
    22741 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccagat
    22801 gaatgactac atagtgcttc gacgtcgttt tatgcaggct gacataatag gtatcgtctt
    22861 tttcttcgta gatcagtgta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
    22921 gataaggaag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gaatcatttg
    22981 cccagacaaa gctgggttca acgttatcca gcagttccgg ataccagtta cctgtttcca
    23041 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
    23101 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
    23161 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
    23221 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
    23281 ttatgtaggg cgcagaaact tctctttgcg gaatgcggtc gatgatttcc tttaagatgc
    23341 gatcctgcaa ggcttgttgc gaggccatca cccgatgacc gtaactattt tcttgttgca
    23401 ggtagtccag gacttccggc tgagaacgcg tatcgtcccg cagccagtag taattatcga
    23461 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
    23521 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg agatgcaagc
    23581 gaaacagggt agtcattgct tagcgaaaaa cgccgtaaac cctcgcccaa tgcgggcggg
    23641 gatataaggc gaaaagcccg cagggcttta aagatcaatc aggtgttgac tgactctgaa
    23701 cgtaggctct gagcgtttca atcgttgcct ctccggtgct acagacaaag tacgatctcg
    23761 accacaaaag ccccgtttta ctctgcatgc gtaaatgcgc gttctgctgg cgtagcaaac
    23821 gtgatgatac cgattttaaa ttattgacca tcacactgac cgccagtttt ggcgggtagg
    23881 caaccagcag gtgtacatgg tctggctccc cgtccatctc aataatttcg cattcaagct
    23941 ttgccgcagc ggagccaaaa gcctcacgca actgagcgat catctgactg tcgaatagtt
    24001 tgcgtcgata ctttgtcgta aaaataaggt gcacaaccag cttactgaca ctgtgtcgtt
    24061 tgcggagaaa tcccgccagt aaatcatcat gattactcaa ttgatatatt tacctaaagt
    24121 gttaaaataa aagcattata tcaatgagcg ctgattatgt taagagcaac aaaagtacgc
    24181 atctacccga caccagaaca ggctgaattt atcaacgccc agttcggtgc agtgcgtttt
    24241 gcgtacaaca aagctctgca tatcaagaaa cattcttacc agcggcaccg cgtaaattta
    24301 aacccgcgta aagacctcaa accactgctt gcagtggcga agaaatcccg ccggtatgga
    24361 tggcttaaag aatatgattc tattgcgttg cagcaggcgg taattaacct cgacgtggct
    24421 ttttctaact ttttcaatcc gaaactgaaa gcaggtaaac ctacctttaa aagtaaacac
    24481 ggcagacaat cgagctacca ttgtattggc ataaaagtcc ttgatgctgc gataaaaata
    24541 ccgaaaatct tgccgattga agcgcgttta catcgtgaaa ttacgggaat gctgaaaagt
    24601 atcacgttga gccgcagcgc aaccgggaaa tactacgcgt cgttactctg tgacgacggg
    24661 atggaagcgc cggcaaagcc cgcactgata tcaaatgtga cagggctgga tatggggctg
    24721 gcgcattttg tcatcaaatc ggacggcaat aaaattgcca gtccccgcca cctaatcaat
    24781 gcaagtcgca acctgcgccg gaagcagaaa gcactgtccc gtaagaaaaa agggagcgca
    24841 aacaggcgca aagccagaat acaacttgca gccgttcacg aacgggtagc cggtgcccgt
    24901 gccgatttcc agcacaagtt atctcgcgca attgttgacg aaaaccaagc ggtaattgtc
    24961 gagacattga aaatggcaaa tatgatgaaa aatcaccatc tggctcgtgc gataggcgat
    25021 gctggatggt ctggattcat cacaaaactg gaatacaaag caacagagaa aggtgtgcat
    25081 ctggtgaaac tggatcaatg gttcgccagt tcgaagacct gccactgctg cggtcacaaa
    25141 atgtcggaga tgccattaca taagcgcatc tggcagtgtc cagagtgtgg agttgaacat
    25201 gaccgcgata tcaacgcggc gctcaacatc aaacgcaagg gcatactgga attacaagcg
    25261 gcgggactcg tcgtttctgc ccatggaggc cagcgtaaat ccgtcacaca gacggttgcg
    25321 gcttaagaag tgggaagcct cgcccgacag ggcggggagc agtcacatga tgacaggtaa
    25381 tggcgcggat atcgaatgtt atgcagacag agaaaatcag cctgctcaaa tggctgtgcg
    25441 gttctggata gcccgaaata gtcaacttca ggctatccag agagcggaat tattccgcca
    25501 aagtgcgttt ttgttgttcg agatcgcgtt caatgccttc acgaacgtcc tgggggattt
    25561 tcagcgcgtc acccagtgca ttcaggtaac tgcgttccat aaaatggtca atatcaatag
    25621 ccgcgcagct caggaaatag atttccagcg cctcttcttc attgcggacc ccggtagcca
    25681 ggcgttgtgg atccagcggt tgttcgattg cctgctcaat gagtacacgc ccctgctctt
    25741 ccacgccggc ttcacgcaat tgctgatcga ttgccgcacg ttctttggca tcaatatgac
    25801 catcactttt agcggcaaag accagcgcaa ggatcaaacg ttcagtacgc tcatccagcg
    25861 gcgtactttg cgcgccaaac tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt
    25921 atttattcca cagcaccgta cccgctaccg ctccgccgcc aaccagcaac gcgttggtgc
    25981 catatttcgt aagaagttta cgtgctgatt tatttgcgac cagcagccca gccagtccgc
    26041 ctaatgcgcc cggcactaaa agtttgccca atccctgatc cgcagacgag gaggcagaag
    26101 aactgctttg cccaagcagg gattgcagtt gatttaacca gttagccata tttgctcctc
    26161 aataaccatt tttagtgcct gtagcatagc ggatgaggat gtcagaaaat gtatatcgag
    26221 acaaaagatg cgcaaataag cggggcggtt ggccgttatt caaagaaatt cccgccaggt
    26281 aaggtgttca ctgtatatca gatgataagg gtattttagg tgaaagtagt ttgacgtttt
    26341 gagtagcact gtttgatgct gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac
    26401 gattgtggtg cgttgtgggg cggataaggc actcgcgccg catccgccaa cggtcaatcg
    26461 aattatcaga agccattaac gccgctgata ttccgccatt cccgctttgc agtccacgct
    26521 gacctgataa tgaatatcgg cacttttacc acgcacggtt agcggtactg accatttatc
    26581 atctttaccc tgaatgtcct gcaaactgac ccaagccacg ggatcggcct gaccgacaat
    26641 tttttgatca tctgcccagc gcgccacgcg attttgttga taatcacgtt ttacgctcgc
    26701 ggcaattccg gcagcatcca gaccttcaca ctttgggaaa gtgaccgact tgctggtttc
    26761 attattggca gcgaaaactg atgcgcaggc agaaaccaac aacagcccta aaaacgcccc
    26821 tctttttttc atgtttttct ccatagcaca atgattcagg agaaagcatg gtacaaattg
    26881 tcaggagcgc aagttgcttc aggccgcgtg tgaggcatct tcctcttcgg attcagcacc
    26941 gacgacctgt ggggacggta atttgccggt tttcagaatg gtgctcagca catctttttg
    27001 ttctgccagc catagcgaaa gggcttcacg ttgctcgtct tccatttgca ctggagaatt
    27061 tgtcagccag gtgtcaagca gatccgccgt atcaagcatt ttgtcataag catcggcttc
    27121 ctttttgctg gtaaacgaca ttttctcttc gccctcacga atgactacgt atttaacttc
    27181 aaccgccatt tgcagcctct cataataact gtgattttat acagtatatt tcttttcggt
    27241 tggaaaatca atatcagcaa taaagacacg cgcaaacgtt ttcgtttata ctgcgcgcgg
    27301 aattaatcag gggatattcg ttatgacgtt attaggcact gcgctgcgtc cggcagcaac
    27361 tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg
    27421 tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac gcgccagcta tgcatgtcgc
    27481 gcatcgctcc catgtcatta atatgcttga tggtgatgca ttacgccgtg tggttgaact
    27541 ggaaaaacca cattatatcg tgccggagat cgaagctatt gccaccgata tgctgatcca
    27601 acttgaagag gaaggactga atgttgtccc ctgcgcgcgc gcaacgaaat taacgatgaa
    27661 tcgcgagggt atccgtcgcc tggcggcaga agagctgcag ctgcccactt ccacttatcg
    27721 ttttgccgat aacgaaaacc ttttccgcaa ggcggttgct gaaatcggct atccctgcat
    27781 tgttaaaccg gtgatgagtt cctccggcaa ggggcagacg tttattcgtt ctgcagagca
    27841 acttgctcat gcatgggagt acgctcagca aggcggtcgc gccggagcgg gccgcgtaat
    27901 tgttgaaggc gtcgttaagt ttgatttcga aattaccctg ctaaccgtca gcgcggtgga
    27961 tggcgtccat ttctgtgcac cggtaggtca tcgccaggaa gatggcgact atcgtgaatc
    28021 ctggcaacca cagcaaatga gccagctggc tttggaacgt gcgcaagaaa ttgcccgtaa
    28081 agtggtgctg gcactgggcg gttatgggct gtttggtgtc gagctgtttg tctgtggtga
    28141 tgaggtgatt ttcagtgagg tctcccctcg cccacatgat accgggatgg tgacgttaat
    28201 ttctcaggat ctctcggagt ttgccctgca tgtgcgagcc tttctcggac ttccggttgg
    28261 cgggatccgt cagtatggtc ctgcagcttc tgccgttatc ctgccacaac tgaccagtga
    28321 gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca ggcctacaga ttcgtttatt
    28381 tggtaagccg gaaattgatg gcagccgtcg tctgggtgtt gcgctggcga ccgctgagag
    28441 tgttgatgaa gcgattgagc gcgcaaagca cgccgcagca cagataaaag tacaagctta
    28501 accccggcga aaaacgctac caaaatgccc gatcgaggat cgggcatttt gacttttaca
    28561 gcttagcgcc ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt
    28621 ccagcgcatc tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca
    28681 ggtagtcacg gtagttagcc ggagaaatac cacccgtcgg gcagaaacgg acctgggaga
    28741 acggacccgc gatcgcctgc agggctttca caccgccgtt agcttcagcc gggaagaatt
    28801 tgaactcttt caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg
    28861 gaatcagagg aatagtccct tcggtagcag ccttcagcag cggctcggtc agacccgggc
    28921 taattgcgaa ctgtgcaccc gcttcggtga cttctgccag ctgctgtgga ttcagcaccg
    28981 taccggcacc cacaatcgct tcaggcactt ctttggcgat agcacggata gcgtcaactg
    29041 cacactcggt acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg
    29101 ccatcggcac cgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg
    29161 tcaggattga ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta
    29221 caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt
    29281 cacgcaaggc gctgaataat tcacgtcctg ttcccacgcg tgacgcgctc aggtcaggaa
    29341 tgtgtggttc gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct
    29401 gtccattcac acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg
    29461 cttctggtgt tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat
    29521 cagtaactaa cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt
    29581 tatgtaattc tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat
    29641 cgcggtccag caaacccgct tcaaaagccg gcataacgtc atgctggctt tcaaaaacaa
    29701 ccgcaggcgc ttcaatcacc tggttctcaa ccggcacggc agaggttttc ataaccgcac
    29761 ggcccaggtt accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg
    29821 aagcgatcac attgctgtcg agtgattttt ccgccccttc ccgccagtcc agttcaccat
    29881 tattcagcca tggttcaagg gtataacgag acagaccaaa accagccacc gtattgacat
    29941 cttcatgcag caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg
    30001 cctggaagtg gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa
    30061 catcagaaag gtcagagaag tcatcccagt taatctgaat accggccgcg cgagccatcg
    30121 ccaccaggtg catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt
    30181 tcaccaccac tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg
    30241 tcatgcgtgt aacctggcgc gcagcggctg cggtcaaagc atcgcgcagc ggagaatccg
    30301 gatgaacaaa agaagagcct ggcaactgca tccccataaa ctccaccacc atctggttgg
    30361 tgttggcagt accgtagaaa gtacatgttc ccggcgcatg ataagacgcg gcttctgact
    30421 ccagtaatgc catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt
    30481 ctttatttgg caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgcc
    30541 caaacgacag ggctgccata gtcagacccg gaacaatctt gtcgcacact ccgaggaaca
    30601 gcgcaccatc aaacatgtta tgggacaacc ccaccgccgc agacatcgca atcacttcgc
    30661 ggcttaacag cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg
    30721 gaacaccgcc cgcaacctga ccaaccgcat ttgcttcatg cagggcttta cgaatgattt
    30781 ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc gttataggag gtgatgatgg
    30841 cgatattgtt acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga
    30901 aaccgtgtgc caggttacca catgccaact gcgaacgatg aacggtcgaa gttttcgctt
    30961 gttctatccg ggcgaggtaa gcagagcgag tctcgcgcga acgttcaatg attcgatttg
    31021 ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg
    31081 attcacaacg cgcttcattc agaggattta tgactgaaac gcctgtaacc ggagctcata
    31141 gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc
    31201 ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca tgttaccggt aaaataacca
    31261 taaaggataa gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca
    31321 ttgccaccga ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt
    31381 ccatcgccca cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcatcac
    31441 gacgaacaaa cagtgcctga ataccacgca tggtttccag cagcagacgt tcataggcat
    31501 ccgccagatg cgtctgatta aaggtttctg aatagctcag gtccagcttg gtgatttgca
    31561 ggttatgttt gtggtcaagg ccaggaactt tattcagtac ctggatatcc acgccttcat
    31621 caggttgcag acggatagtc agtttatttt gcggtagatc ctgccacgac tctttaaaca
    31681 gattcagttc aggtgttttg aaatagacca cgacttcaga gcatttggtc ggcagacgtt
    31741 taccagtacg caggaagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga
    31801 tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc ctcttcttcc agatatcccg
    31861 gcactttttt gccctgggcg aagcccgcag tatattgccc gcgtacggtt ttttcgcgta
    31921 cgttggagcg gtcaatacgg cgcagagact tcagtacttt cactttttca tcgcggatgc
    31981 tgtctgcact caggtcagac ggcggagaca tcgcaatcat gcaaagaatt tgcagcaggt
    32041 ggttctggat catgtcgcgc atctgaccgg ctttatcaaa atagccccag cgcccttcga
    32101 tccccacttc ttctgccacg gtaatctcaa catgatcgat ggtgcgattg tcccagttgt
    32161 tcacaaacag agagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa
    32221 gatagtggtc gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcgttga
    32281 tttcctgcga ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt
    32341 tagcattcag ttttgcctcg ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca
    32401 tggcaaagta gttaatggtg atacgatttt tttgatccag catcgcgccg agacggctga
    32461 atgcagcagt gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc
    32521 ataaaccttc atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg
    32581 tataagccgc tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga
    32641 gctgaccggc tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc
    32701 ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat
    32761 tctccttaag ttaactatcc cggtacttaa gccagggtat acttgtaatt ttcttacggt
    32821 gcactgtact gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta
    32881 cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaaa tcatcgtttt ccattgggta
    32941 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
    33001 ttaacacagc acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagtgcgg
    33061 aaatcgtcat tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc
    33121 agctggaaca tttgagcaaa tcagagcgca aagttgccga ggtcattctg gcttcgcccg
    33181 ataacgcgat ccattcgagt attgctgctc tggcgctgga agccaatgtt agcgaaccga
    33241 cggtgaatcg tttctgtcgc agcatggaca cgcgcggttt tcctgatttt aaacttcatc
    33301 tggcacaaag tctggcgaat ggcactccct atgttaatcg caacgtcaat gaagatgaca
    33361 gcgttgaatc atacacgggg aaaatatttg agtccgcaat ggcaacgctc gatcatgtcc
    33421 gtcattcatt ggataaatct gccatcaacc gcgccgtcga cttactcact caggcaaaaa
    33481 aaatcgcctt tttcggatta ggctcttctg ccgctgttgc ccacgatgcg atgaataagt
    33541 tctttcgttt taatgttccg gtggtgtact ccgatgatat tgtgctgcaa cgcatgagtt
    33601 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacacggga agaacaaaaa
    33661 atctggtcga gctggcgcag ctggcacgcg aaaacgacgc catggtgatt gccctcacct
    33721 ctgcaggtac cccgctcgcc cgggaagcca cgctagcaat taccctcgac gtaccggaag
    33781 atactgacat ttatatgccc atggtttctc gacttgcaca gctgaccgtg atagatgtgc
    33841 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
    33901 aggaagcgct gaaggaatcg cgttttgata agcagttact taatttaagt gacgatcgct
    33961 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatagcca
    34021 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
    34081 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
    34141 acagatcgcg ataataatct tgaaaaagtt atcgcagcgg gtgccaacgt tgtacgtatg
    34201 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgaaatt
    34261 gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaattcgt
    34321 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    34381 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    34441 cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt ccagttaaaa
    34501 gtactggaag ttcagggcat gaaagtgttc accgaagtca ccgtcggtgg tcccctctcc
    34561 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    34621 gacaaagcag acattaagac tgcggcgtta attggcgtag attacctggc tgtctccttc
    34681 ccacgctgcg gcgaagatct gaactatgcc cgtcgcctgg cacgcgatgc aggctgtgat
    34741 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    34801 gacatcatcc tcgcctctga cgtggtaatg gttgcacgtg gcgacctcgg tgtggaaatt
    34861 ggcgacccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagcttaac
    34921 cgcgcagtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    34981 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctt
    35041 tctgcagaaa ctgctgctgg tcagtatcct tcagaaactg ttgcagccat ggcgcgcgtt
    35101 tgcctgggtg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgtgcag
    35161 ttcgacaatg tggaagaagc tattgccatg tcagcaatgt acgcggctaa ccacctgaaa
    35221 ggcgttacgg cgatcatcac catgaccgaa tcgggtcgta cggcgctgat gacctcccgt
    35281 atcagctctg gtctgccaat tttcgccatg tcacgccatg aacgtacgct gaacctgact
    35341 gctctctatc gcggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcagct
    35401 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    35461 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    35521 acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc tcatccggcc tacagttcaa
    35581 ttatagttca acagatttcg aatcttctga agcaaacttg aacttatcac aaggcggagg
    35641 cctctcctcg cgagaggctt tttttatttg atgggataaa gatctttgcg cttatacggt
    35701 tggatttcgc ccggcttgcg agttttcagc aattttaata tccaggtgta ttgttctggt
    35761 cgcggaccaa caaaaatctc gacttcttca ttcatccgcc gcgcaatcgt atggtcatct
    35821 gcctctaaca gatcatccat cggtgggcgc acctgaatcg tcaggcgatg cgtcttgcca
    35881 tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca ctttcatcaa acgaccaatc
    35941 gcgggcaacg tcgctttata ggtggcaaag aaatcaacaa attcgctgtg ctctgggcca
    36001 tgatcctgat cgggtaaata atatccccag tacccctgac gtaccgactg gatgaatggt
    36061 ttaataccat catttctcgc atgcagacga ccgccaaaac gacgacgcac cgtgttccag
    36121 acataatcaa aaaccgggtt gccctgatta tggaacatcg ctgccatttt ctgcccttgc
    36181 gaggccatca gcatggcagg aatatcgacg gcccaaccgt gcggcaccag aaagatcact
    36241 ttctcgttat tacgtcgcat ctcttcgata atctccagcc cttgccagtc aacgcgcggc
    36301 tgaattttct ccggcccgcg tattgccaac tcagccatca ttgccatcgc ttgcggcgcg
    36361 gtggcaaaca tctcatcaac aatcgcttcg cgttcagctt cactacgttc tggaaagcag
    36421 agcgacaaat tgattaacgc acgacggcgt gagctttttc ccaggcgtcc ggcaaaacgt
    36481 cccagccgtg ccagaatggg gtcgcgaaac tttggcggcg ttaaagcaat acccgccatc
    36541 gctgctacgc ccagccatgc tccccagtag cgcgggtggc gaaaggattt atcaaactca
    36601 ggaatgtatt cgctattatt ttttttcgtt tccatgcttt tccagtttcg gataaggcaa
    36661 aaatcaatct ggtgatagtg tagcggcgca acttgccccg caccaaataa aaaaagccgg
    36721 tactgactgc gtaccggctg cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa
    36781 tctctttggc ctgcgccagg aattcgcgac gatcggagcc ggtcagccct tcggtacgcg
    36841 gcagttttgc cgtcagcggg tttacggcct gctggtttat ccatacttca tagtgcagat
    36901 gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat acggtcgcca cgtttcacct
    36961 tctgtcccgg tttcaccaga atcttgcgca agtgcatata acgcgtggtg tagctgcgac
    37021 catgacgaat agccacataa taacctgctg cgccactgcg tttggcaacc accacttcac
    37081 cgtcacccac tgaaagcacc ggcgtaccct gtggcatggc gaaatcaaca cctctgtgtg
    37141 gcgcaacacg accagtcacc ggattagtac gacgcgggtt aaagttagac gagatacgga
    37201 actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag accagtaccg ttacgatcat
    37261 agaatttgcc atcttcagcg cggattgcgt aataatcttt accttctgaa cgcaaacgta
    37321 cgcccagcag ttggctttgc tcacgtttac catcaagcat ttctcgtgac attaacaccg
    37381 caaattcatc gccttttttc agtttgcgga aatccatttg ccactgcatg gctttaatca
    37441 ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc gctggcaaca aagcttcccc
    37501 cgacggtacc tttcagcaga ttgttgaccc actctccttg ctgcatttcg ctggtcattt
    37561 taaaaccgtt agcggcagta cggtcatagg ttcgggtttc acgacgagac acttcccagg
    37621 tgaggcgctg cagttcgccg tccgcggtta atgtccagga gagttgttga ccgattttca
    37681 ggttacgcaa ttctttgtcg gcagcagcca gttgggtgat atcacccata tcaataccat
    37741 actgattgag aatgctgctt agcgtatcgc cagtggaaac aacatattca tgcacgcccg
    37801 cttcaccggc gattttgtca tccagttcat cctggggaat ggcttcatct tcttgtgcag
    37861 cttgatcaat cggctcactg gcttcaggta agagcgaacg aatttcgttc tgttccagct
    37921 caatggtttt gacaattggc gtggcatcgc ggtgataaac atagggccgc cagacagcga
    37981 cggccagagt aagaacggtg agcgacccca acataacgcg gtgtggtcgc ggtaaattat
    38041 taaacgccag ggcgacagag cgggctatct gttgcacgta atcacttcct cattaatctc
    38101 ctttcaggca gctcgcatac tggttggcta attgattcag gaattctgaa tagcttgttt
    38161 tacccagttt gatattcgtc cccaggggat ccaacgttcc catacgaacg gatgtccctc
    38221 gtgcgacgct ctcaacgacc gctggcctga actgtggctc agcaaaaacg caggttgctt
    38281 tttgctcaac caactgtgtt cttatttcat gtaaacgctg cgcgccaggt tgaatctcag
    38341 ggttaacggt aaaatgacca agcggtgtca gtccgaactg tttttcgaaa tagccgtaag
    38401 catcgtgaaa aacgaaataa cctttcccct tgagcggcgc gagctcgtta ccaacctgct
    38461 tttcggttga ggctaattgt gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac
    38521 tttgcggcat aagttccact aattttccat ggattgcaac cgctgtagcc cgcgctatct
    38581 ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg gtgatcttcg tcactttttt
    38641 ccgcgtggtc gtgatcatca tcatcgccgt gaatactttt catcagcagc ggtttcacat
    38701 tctctagctg cgcaatcgtt acctgtttcg cttcaggtaa tttacttacc ggtttttgca
    38761 tgaacgcttc catctccggg ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta
    38821 catctgatgg acgcagtgaa taatcatgtt ctgaagcgcc gtcaggtagt aaaacctccg
    38881 tttctgttac cccatcagca atggcagaag cgatgaaccc aacgggttta agcgaagcga
    38941 caacggcagc atctgcggcc tgtgttgcac cgccccagag agcggcggat aatgctgcga
    39001 aaagaagcgt ttttttatgt aacataatgc gaccaatcat cgtaatgaat atgagaagtg
    39061 tgatattata acatttcatg actactgcaa gactaaaatt aacatgacaa gtctggtttc
    39121 cctggaaaat gtctcggttt cttttggcca acgccgcgtc ctctctgatg tgtcgctgga
    39181 acttaaacct ggaaaaattt tgactttact tgggccaaat ggcgcaggta agtcgacact
    39241 ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa
    39301 actgcgcatc ggctatgtac cgcagaagct gtatctcgac accacgttgc cacttaccgt
    39361 aaaccgtttt ttacgcttac gccctggcac acataaagaa gatattttgc ctgcactgaa
    39421 acgtgtccag gccgggcatc tgattaacgc accgatgcaa aagctctcgg gtggcgaaac
    39481 acagcgtgta ttgttagcgc gagcattgtt aaatcgaccg caattattag tgctggatga
    39541 acccactcag ggcgtggatg tgaatggtca ggtggcgtta tatgacctta ttgaccagtt
    39601 gcgtcgcgaa ctggattgtg gcgttttaat ggtctctcac gatctgcatc tggtaatggc
    39661 aaaaaccgat gaagtgcttt gcctgaatca ccacatttgt tgttccggta caccggaagt
    39721 tgtttccctg catccggagt ttatttctat gtttggtcct cgtggtgctg aacaactggg
    39781 tatctatcgc catcatcata atcatcgtca cgatttacag ggacgaattg ttttgcgtcg
    39841 gggaaatgat cgctcatgat tgaattatta tttcccggtt ggttagccgg gatcatgctc
    39901 gcctgtgccg cgggtccgct gggttcgttt gtggtctggc gtcgtatgtc ttatttcggt
    39961 gatacgctgg cccatgcctc attacttggt gtcgcgtttg gtttgttgct ggacgtgaat
    40021 ccattctatg cggtgattgc agttacgctg ctgctggcgg gcggtctggt atggctggag
    40081 aagcgtccac agctggcgat cgacacgtta ttagggataa tggcgcacag tgccctgtcg
    40141 ctgggcctgg tggtcgttag tctgatgtct aatattcgtg ttgatttgat ggcttacctg
    40201 ttcggtgatt tgctggcagt gacgccagaa gatctcatct ctattgcaat tggcgtggtc
    40261 atcgtggtgg cgattttgtt ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg
    40321 gatctggcgt ttgttgatgg tgtgaaatta cagcgcgtga aattgttgtt gatgctggtg
    40381 acggcattga cgattggtgt agcgatgaaa tttgtcggcg cgttgattat tacttcgttg
    40441 ctaattattc ctgctgctac tgcacgtcgc tttgcccgca cgccggaaca gatggcaggt
    40501 gtcgctgttt tggtggggat ggtggcagtg actggcggtt taaccttttc cgcattttac
    40561 gatacaccag caggcccgtc ggtggtgcta tgcgcggcac tgttatttat tctcagtatg
    40621 atgaaaaaac aggccagcta atctttcgct gaacacattt gtcggatgcg gcgcgagcgc
    40681 cttatccgac ctgtggttcg ctatctctgg tatatgcctg ataagacgcg aatagcggcg
    40741 catcaggcaa cactgccagt gtcggatgcg ccgcgagcga ccgatccgac ttacggcatt
    40801 tctggcggcg tgatgccgaa gtggttccac gcccgcactg tcgccatacg cccgcgcggt
    40861 gtacgctgca aaaagccttg ctgaatcaaa taaggttcca gcacatcctc aatggtttca
    40921 cgttcttcgc caatggcagc cgccaggtta tccagaccta ccggcccacc aaagaactta
    40981 tcgattaccg ccagcaacaa tttgcggtcc atataatcga aaccttcggc atcgacattc
    41041 agcatatcca gcgcctgagc agcgatatca gccgagatgg tgccatcgtg cttcacttcg
    41101 gcgaagtcac gcactcgacg cagcagacgg ttggcaatac gtggcgtacc gcgagcgcga
    41161 cgagcaactt ccagcgcgcc gtcatcactc atctcaagcc ccataaagcg tgcgctgcga
    41221 ctgacgatat attgcagatc cggcacctga taaaactcca gacgctgcac aataccaaaa
    41281 cgatcgcgca acggtgatgt cagcgaacct gcgcgcgtgg ttgcaccaat cagggtaaac
    41341 ggcggcaaat caattttaat ggagcgtgcc gccggacctt caccaatcat gatatccagt
    41401 tggtagtctt ccattgccgg gtacagcact tcttcaacaa ctggcgatag acggtggatc
    41461 tcatcaataa acagcacgtc atgcggttca aggttagtga gcatcgctgc cagatcgccg
    41521 gccttttcca gcaccggacc agaagtcgtg cgtaaattaa cgcccatttc attggcgaca
    41581 atgttggcaa gcgtagtttt acccaacccc ggaggaccaa aaatcaacag atgatcgagg
    41641 gcatcgccgc gcagtttcgc tgctttgatg aaaatctcca tctgtgaacg aacctgcggc
    41701 tgaccaacat actcttccag taatttaggg cgaatggcgc gatctgccac atcttccggc
    41761 aaagtggtac cggcagaaat cagacggtct gcttcaatca tcctttacct cataacgcgg
    41821 cgcgtagggc ttcgcgaatt aatgtttcac tgctggcgtc agggcgagcg attttgctca
    41881 ccatgcggct tgcttcttgt ggtttatagc ccagcgccac cagcgcggca accgcttctt
    41941 gttcagcatc gtcggtcgcc gggctggcag gagacgtgag taccaggtcg gcggctggcg
    42001 taaagagatc gccatgcaaa cctttaaatc ggtctttcat ttcaacaatc aagcgttcgg
    42061 cggttttttt gccaataccc ggcagtttca ccagtgcccc cacttcttca cgctcaacgg
    42121 cattaacgaa ctgctgcgct gacattccgg agaggatcgc cagcgctaac ttcgggccga
    42181 cgccgttggt tttgatcaac tctttgaaca atgtgcgctc ttgtttattg ttaaaaccgt
    42241 acagcagttg cgcgtcttca cgcaccacaa agtgggtgaa aacgatcgct tcctgacccg
    42301 cttcagggag ttcataaaaa caggtcatcg gcatatgcac ttcatagcct acgccgccca
    42361 cttcaattaa caccagcggg ggttgttttt caatgatgat gcctctgagt ctgcctatca
    42421 catgacgctc ctgcgtaatg aatcaaagat aatgctgtat gataaaaaaa tgctggatag
    42481 atatccagcg aaggatgaag aaaacttgcg aggtgtcccg atg
//
LOCUS       DAOTRK010000019        40639 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.019, whole genome shotgun sequence.
ACCESSION   DAOTRK010000019 DAOTRK010000000
VERSION     DAOTRK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.019"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(58..3960)
                     /gene="hrpA"
                     /locus_tag="QIE41_001092"
     CDS             complement(58..3960)
                     /gene="hrpA"
                     /locus_tag="QIE41_001092"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDS3530006.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRQRFLRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKKDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTSNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4161..4766
                     /gene="azoR"
                     /locus_tag="QIE41_001093"
     CDS             4161..4766
                     /gene="azoR"
                     /locus_tag="QIE41_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDS3530007.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(4880..5740)
                     /gene="pdxI"
                     /locus_tag="QIE41_001094"
     CDS             complement(4880..5740)
                     /gene="pdxI"
                     /locus_tag="QIE41_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDS3530008.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALHPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDDLARVGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(5948..12439)
                     /locus_tag="QIE41_001095"
     CDS             complement(5948..12439)
                     /locus_tag="QIE41_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDS3530009.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEVETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECTPLVEQNLMPWLATGAAALITTLAVVELNDDDDHHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDVPDDTPTPPKPISFNNDVILDKTEKTLTIRDS
                     VFTYTENADGTISLQDNSGRKATINLWQIDEANNTVALEGVSADGTTKWRYNHNGELV
                     ITGDNATVNNNGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVD
                     NKGTMTVTDPESIGIQIDGDKAVVNNEGDSTISNGGTGTQINDDDATANNNGKTTVDG
                     KDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESMGIQID
                     GDKAIVNNEGESTITNGGTGTQINGDDATANNNGKTTVDGKDSTGTEINGNNGKVIQG
                     GDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESIGIQIDGDKAVVNNEGDSSISNGG
                     TGTQINGDDATANNSGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDS
                     ATVDNKGTMTVTDPESIGIQIDGDKAIVNNEGDSTISNGGTGTQINGDDATANNNGKT
                     TVDGKDSTGTEINGNNGKVIQDGDLDVSGGGHGIDITGDSATVDNKGTMTVTDPESMG
                     IQIDGDKAIVNNEGESTITNGGTGTQINGDDATANNNGKTTVDGKDSTGTEINGNNGK
                     VIQDGDLDVSGGGHGIDISGDNAAVDNKGNMTVTDADSIGVLINGDNATLANTGDVDV
                     SNSATGFAITSDEGKISLAGSMQVGDFSTGLTLTGDNNSLTMAMQDLNVVGQKATGIN
                     VSGDANTIDITGNILVDKDQSADNAAEYFYDASVGVNVSGHNNNVTLDGKLTLVADGE
                     TTTRDVSNFDGSQENISGLIVSGDGNNINLNGGIAFVGEINALTDGSAIANKRTFFGN
                     TPLIGIDGESKVYLNGDSTISGDLQLGYASLIQLKNKAAIEIGSEATFNMRDIENYDH
                     YYAQTPQIIKAESSSEVINNGNVDIWNISFAGIFGENTTGINNGNITLSLYDYASTNT
                     PAPEPDNTAFLTSNGGSAVNKGVITSKVMEQHSVVNMAALTGSTDQRVFNNSVASMMG
                     MEAYNKGSVLNAEDAVIDMYGRGSIDMLAIDNSTADNAGNITVDTLWIDDNDTTSLHT
                     DLPGATAKDYGVGMATGTDTGGGARNNAIATNLEGGVITVYNAGAGMAAYGNSNMVIN
                     QGIINLEKNADYDANLGSNTLVGMAVYKGATAINDQTGVININVDTGQAFYNDGTGII
                     LNYGEINLNGAEIDSTDSHYGAPAENLELLSELSASGENITKTVIRDGFVTIKPLANY
                     GTEILNGDVDANLWLYNEDKASLTVNGDLNIVQGLENSGSMDADKLTANASVYNRASG
                     SMTTELLMLKGGSAFFNEGSFSGVISGDSYKQNVVNTGEMTTVTDDSALINGSFVLYN
                     EAGSTLTNSGNAIAGGENAIVNITRTSDSLSQVNRGKITATNGYSAIKTASTASNSNG
                     KWIWNTETGVINGINPDAPLIDLGRGYNFANAGTINVQGDGSVAISGGTTSYTVQLVN
                     SGTINVGTEQGKADGSNGEGLIGIKGNGSATTINNTKDGVINVYADNSWAFGGSTKAI
                     VNNGIINLLCNIGCEIYAPNTTGTRNSQDGTADIIVPVASATPGQGNVPAAPVNAVSQ
                     QKLTNYTIGTNSDGSSGTLKANNLVISDNVKVNTGFSAGTADTTVVIDDVFKGENISG
                     AENITSSSVVWNAKGSTDASGNVDVTMSKNAYTDVATDASVNDVAKALDAGYTNNELY
                     TSLNVGTTAELNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAK
                     GDPRAELGNNTEYDMLALRKTIDLSESQTMSLEYGIARLDGDGAQKAGDNGVTGGYSQ
                     FFGLKHQMSFDNGMNWNNALRYDVHNLDSSRSIAFSNTNKTADTDVKQQYLEFRSEGA
                     KTFEPSEGLKVTPYAGVKLRHTLEGGYQERNAGDFNLNMNSGSETAVDSIVGLKLDYA
                     GKDGWSASATLEGGPNLSYAKSQRTASLAGAGSQHFNIDDGQKGGGINSLASVGVKYS
                     SKESSLNLDAYNWKEEGISDKGVMLNFKKTF"
     gene            complement(12770..13360)
                     /gene="paaY"
                     /locus_tag="QIE41_001096"
     CDS             complement(12770..13360)
                     /gene="paaY"
                     /locus_tag="QIE41_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDS3530010.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            complement(13342..14292)
                     /gene="paaX"
                     /locus_tag="QIE41_001097"
     CDS             complement(13342..14292)
                     /gene="paaX"
                     /locus_tag="QIE41_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDS3530011.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            complement(14393..15706)
                     /gene="paaF"
                     /locus_tag="QIE41_001098"
     CDS             complement(14393..15706)
                     /gene="paaF"
                     /locus_tag="QIE41_001098"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDS3530012.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPQDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(15733..16938)
                     /gene="paaJ"
                     /locus_tag="QIE41_001099"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(15733..16938)
                     /gene="paaJ"
                     /locus_tag="QIE41_001099"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDS3530013.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(16938..17360)
                     /gene="paaI"
                     /locus_tag="QIE41_001100"
     CDS             complement(16938..17360)
                     /gene="paaI"
                     /locus_tag="QIE41_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDS3530014.1"
                     /translation="MSNKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(17353..18777)
                     /gene="paaC"
                     /locus_tag="QIE41_001101"
     CDS             complement(17353..18777)
                     /gene="paaC"
                     /locus_tag="QIE41_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDS3530015.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYE"
     gene            complement(18779..19567)
                     /gene="paaB"
                     /locus_tag="QIE41_001102"
     CDS             complement(18779..19567)
                     /gene="paaB"
                     /locus_tag="QIE41_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDS3530016.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(19567..20334)
                     /locus_tag="QIE41_001103"
     CDS             complement(19567..20334)
                     /locus_tag="QIE41_001103"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDS3530017.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNVLLTQLVNELEAAAID
                     PSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(20331..21401)
                     /gene="paaK"
                     /locus_tag="QIE41_001104"
     CDS             complement(20331..21401)
                     /gene="paaK"
                     /locus_tag="QIE41_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDS3530018.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLNGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAEAALKALGMPEKAIHLERFNTAGTRVKRNVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(21409..21906)
                     /gene="paaD"
                     /locus_tag="QIE41_001105"
     CDS             complement(21409..21906)
                     /gene="paaD"
                     /locus_tag="QIE41_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDS3530019.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTNGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(21921..22667)
                     /gene="paaC"
                     /locus_tag="QIE41_001106"
     CDS             complement(21921..22667)
                     /gene="paaC"
                     /locus_tag="QIE41_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDS3530020.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESREPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGFNEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMVAEMQYLQRVLPGQQW"
     gene            complement(22676..22963)
                     /gene="paaB"
                     /locus_tag="QIE41_001107"
     CDS             complement(22676..22963)
                     /gene="paaB"
                     /locus_tag="QIE41_001107"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDS3530021.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(22975..23904)
                     /gene="paaA"
                     /locus_tag="QIE41_001108"
                     /pseudo
     CDS             complement(22975..23904)
                     /gene="paaA"
                     /locus_tag="QIE41_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
     gene            24188..26233
                     /gene="paaZ"
                     /locus_tag="QIE41_001109"
     CDS             24188..26233
                     /gene="paaZ"
                     /locus_tag="QIE41_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDS3530022.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGGRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQVLVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWSVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            26481..28754
                     /gene="tynA"
                     /locus_tag="QIE41_001110"
     CDS             26481..28754
                     /gene="tynA"
                     /locus_tag="QIE41_001110"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDS3530023.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDTKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSSHDTGLGQYSKDNESLDNTDAVIWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGAIKKDK"
     gene            complement(28813..30312)
                     /gene="feaB"
                     /locus_tag="QIE41_001111"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(28813..30312)
                     /gene="feaB"
                     /locus_tag="QIE41_001111"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDS3530024.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTVDANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHGEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGSVCGAALTSHPHVAKISFTGSTATGKGIARTAADRLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVRSFLDDAQAQQAELIRGASGPAGEGYYVAPTLVVN
                     PNAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            30548..31453
                     /gene="feaR"
                     /locus_tag="QIE41_001112"
     CDS             30548..31453
                     /gene="feaR"
                     /locus_tag="QIE41_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDS3530025.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(31625..31951)
                     /locus_tag="QIE41_001113"
     CDS             complement(31625..31951)
                     /locus_tag="QIE41_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDS3530026.1"
                     /translation="MKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVNDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGING
                     KWVRKF"
     gene            complement(31959..32144)
                     /locus_tag="QIE41_001114"
     CDS             complement(31959..32144)
                     /locus_tag="QIE41_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDS3530027.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(32141..34780)
                     /locus_tag="QIE41_001115"
     CDS             complement(32141..34780)
                     /locus_tag="QIE41_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDS3530028.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVEFDSACLVKL
                     PQTEQSLAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLSQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVVMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            34988..35977
                     /gene="ldhA"
                     /locus_tag="QIE41_001116"
     CDS             34988..35977
                     /gene="ldhA"
                     /locus_tag="QIE41_001116"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDS3530029.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            36088..36510
                     /gene="hslJ"
                     /locus_tag="QIE41_001117"
     CDS             36088..36510
                     /gene="hslJ"
                     /locus_tag="QIE41_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDS3530030.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQLHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGKLTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(36507..36773)
                     /locus_tag="QIE41_001118"
     CDS             complement(36507..36773)
                     /locus_tag="QIE41_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDS3530031.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            37047..40571
                     /gene="nifJ"
                     /locus_tag="QIE41_001119"
     CDS             37047..40571
                     /gene="nifJ"
                     /locus_tag="QIE41_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDS3530032.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELIPQAEIDAHRARGLNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMVERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKTARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDTNGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEIRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         9691 a        10937 c         9802 g        10209 t
ORIGIN      
        1 aaaaaacccg gcgctaaatg cttaccgggt tttagattta tcaggcaaat agcagtgtta
       61 accgctaatc tgctccatcg cctgcaaaat acgcttatct gaaatcggat aaggcgtacc
      121 aagttgttga gcgaagtaac taacgcgcaa ctcttctatc atccaacgga tctctttcac
      181 gtcttcatcc tcacgacgtg cgggcggcag tttgttgatc cattgctgcc acgcctgctg
      241 gacattttcg actttcagca tctgagcacg gtcgcgatgt gggtcaaccg ccagtttttc
      301 cagccgtttt tcaatcgcct gcaaataacg cagcgtgtcg cccaaccgtt tgaagccgtt
      361 actagtgaca aaaccgcgat ataccaaccc gcccatctgc gctttaatgt cagaaagccc
      421 cagcgccatg gtcatatcca cccgcccttt cagacgttta ttgatattga acaccgcagt
      481 aaggatttgc tcgacctgct tcgcaatatc caccaccgtg tcgttcagtt cagcgcggac
      541 tttttcatgc agcgcagcaa aaccttcttc cgtccagacc gggccaccat tggcgtcgat
      601 cagttgatcc acaccgcagg agatacagtc gtcgatcagc tccagcactt tgccatacgg
      661 gttaaagtac agccccagct tggctttgtt tggcaacttc tcatgcaaat atttgattgg
      721 cgagggaata ttcagcagca gtagacggcg aagaccgttc cacattgcct gcttttgttc
      781 cagcggatta tcaaacagtt tgatcgccac gctgtcgcgt tcatccacta gcgctggcca
      841 cgccttcact ttatagttgc cacgcttctg ttcgtagctt tccggcagct gaccaaaact
      901 ccagatatgt aagccgctct gctcgatacc gtcatccgcc accgcagaca gcgtttcctg
      961 cactttgcct ttcagcgcat ctttcagatc ttgtagagag cgcccttctt ttagcttctt
     1021 gtttttgtca tccaccacac ggaaggtgat tttcaggtga tcgggcacct gatcccagtg
     1081 ccagtcttcg cggtcaacgg tgacgccagt catccgccgt agctctcgtt caaggctgtc
     1141 gagcaacggc aattccagcg gtgtgacgcg gcctaaaaaa gcttcggcgt agtttggcgc
     1201 gggcacaaaa ttacgccgta ccggtttcgg caacgattta atcagagcaa tcaccagttc
     1261 gcggcgcaga ccggggatct gccactcaaa tccgctctcc tccacctggt taagtaacgg
     1321 cagcgggata tgtaccgtca cgccgtccgc atccgcccct ggctcaaact gataactcaa
     1381 acgcagcttg agattgccct gatgccagaa gttcgggtaa tccagcttgc tgatcttttc
     1441 cgccccttct ttgatcaaca tgctcttttc aaagttgagc aaatcaggcg tttcgcggct
     1501 gacttttttc caccagctgt cgaagtgacg tgcggagatc acatcgtggc tgatgcgctg
     1561 gtcgtaaaac tcaaacagcg tttcgtcatc caccagaata tcgcggcgac gtgttttgtg
     1621 ttccagttct tccacttcgg cccgtagttt caggttttca cggaagaatg cgtgacgcgt
     1681 ctgccagtca ccttccacca gcgcgtggcg aataaagagt tcacgacata acgccggatc
     1741 gatctggctg tagttgacct tgcgcgcggc aacaatcggc aagccataaa cagtgacttt
     1801 ttccgttgcc atcaccgcgc cctgcgcccg ttcccagtgc ggttcgctgt aggtgcgttt
     1861 aatcaaatgc tgagcaactg gctccaccca ttctggatcg atgcgtgccg caatgcgccc
     1921 ccacaggcgg ctggtttcta ccagttccgc caccattacc catttcggcg gctttttaaa
     1981 taagccggaa ccggggaaaa tggagaaacg cgcgttacgt gcgccggtat attcttgttt
     2041 atcggcatct ttcatgccga tatgggaaag caaaccggtc agtaaggcga tgtgaatttc
     2101 gcgatactcc gccggttcgc tgttaaccgg aatgccaagt tcttttacca cctggcgcaa
     2161 ctgggtgtag atatcctgcc attcgcgcac gcgcagatag ttgagataat cggtacgaca
     2221 caggcgacgg aaagcgttgg aagaaagcgc cttttgctgc tcgccaagat aattccacag
     2281 atttacaaac gcgaggaagt cggattcttt gtcgtggaag cgacgatgtt tttcgtccga
     2341 tgcctgctgt ttgtccatcg gtcgttcgcg cggatcctgg atggagagcg cggacgtgat
     2401 aatcatcgcc tcacgcacgc agccatgttt ttgcgcttcc agcaccatac gcgccagacg
     2461 aggatcgaca ggcaattgcg aaagctggcg accgagcggc gtcagtttat aggcgctggc
     2521 ctgttcatca gtggtgatcg cgcccagctc ttcgagcaga cgcacgccat cctggatatt
     2581 acgtttatcc ggtgcttcga caaatgggaa cgcggcgata tcgcccaacc ccagcgcggt
     2641 catctgtaaa ataaccgagg ccaggttggt acgcagaatc tccggatcgg taaactccgg
     2701 gcgcgagagg aaatcgtctt cggaatagag acgaatacag atcccttcgg acacacgacc
     2761 acagcggcct ttacgctggt tggcagacgc ctgcgaaatg ggctcaatcg gcaaacgctg
     2821 cactttggtg cgatagctgt agcggctgat acgcgccgta cctgggtcga taacgtattt
     2881 aatccccggt acggtcagcg acgtttccgc gacgttggtc gccagcacaa tgcgccgtcc
     2941 actgtgcgac tggaacacgc ggttctgttc gctgttcgaa agccgcgcat aaagcggcaa
     3001 gatttcggta tggcgtaagt tcagcttgtt cagcgcatcg gcggtatcgc gaatttcccg
     3061 ctcaccgctc ataaagatca gaatgtcgcc gggactttcc tggctcagtt cgtctacggc
     3121 gtcaaaaatc gcctgcaact gatcgcgctc ggtgtcatcg gcttcttcaa caatcgggcg
     3181 atagcgcact tccaccggat aggtccgacc ggagacttcg ataatcggcg cattattaaa
     3241 gtggcgcgaa aagcgttccg ggtcgatagt cgcggaagtg ataatgattt ttaggtcagg
     3301 acgccgcggt agcaactctt tcaaatagcc gagcagaaaa tcgatattca ggctgcgttc
     3361 gtgcgcttcg tcaataatga tagtgtcgta ctgcatcagc aggcggtctt gctggatctc
     3421 cgccagcagg ataccgtcgg tcatcagctt gaccatcgtg ttatcactta cgtgatcgct
     3481 gaaacgcact ttgtaaccga tgcaaccgcc cggctccgtt ttcagctctt ccgcaatacg
     3541 gttcgccacc gttcgtgccg ccaagcgacg cggctgggta tggccgatca gccctttaat
     3601 cccgcgcccc agttccatac agattttcgg tagctgagtc gtcttaccgg aaccggtttc
     3661 cccggcgacg atcaccacct ggtgatcacg aatcgcttcg agaatgtcct tctttttctg
     3721 actaactggc aaattgtcgg gataagtgat ttccggtcgt gccgcttcac gcagcaggac
     3781 tttccctgcc gcctggtcaa tctctttcgc catctcctgg aaaatggcct gttgtgcatc
     3841 aggattttta accttcttca caccgtgcag acggcgtaaa aaacgctgtc tgtcgcgcag
     3901 catcagcgaa tccagccgct gctgcaaggc agtaaaggtc aatttttgtt gttctgtcat
     3961 aacgttagtg ggcagtagtc tgcctgatta attttctttt aaatgataga tatagagtac
     4021 cacatcgcgg ctttctatgc gttgttcaat aaattcgaac ataggcttcg atttattgcg
     4081 ctatctctgt ggcaggattg tgaataaagt gtcagcaagc aacggggcat cgcccattca
     4141 aacatctata aggaaacacc atgagcaagg tattagttct taaatccagc atcctggcag
     4201 ggtactctca gtctaatcag ttgtccgatt attttgttga acaatggcgc gaaaagcact
     4261 ccgctgatga aatcaccgtt cgcgacctgg ctgcaaatcc gattccggtg ctggatggcg
     4321 aactggttgg tgcgctgcgt ccgagcgatg caccgctgac tccgcgtcag caggaagctc
     4381 tggcactttc cgatgagctg attgccgagc tgaaagccca cgacgttatc gttattgcgg
     4441 caccgatgta taacttcaac atctcgactc agttgaaaaa ctattttgac ctggttgcac
     4501 gcgcaggcgt tactttccgc tataccgaga atggtccgga aggtctggta acgggtaaaa
     4561 aagccatcgt tattaccagc cgcggcggga tccacaaaga tggaccaacg gacctggtga
     4621 cgccgtatct gtccacgttc ctcggcttta tcggcattac tgatgtgaaa tttgtcttcg
     4681 ccgaagggat cgcatacggt cctgaaatgg cagcgaaagc gcagtctgac gcgaaagcag
     4741 ctatcgacag tattgttgct gaataaccgg acacgatctt gatgtggact gtagatattc
     4801 agtccacatc tcaatccgct tacctgtttt ctatcgcttt gtgctcttcc ttaagcacaa
     4861 tctgcgagcg cctgactcgt tattctcgcg aaataccatc caacgtagac aacacttcct
     4921 cagaaagatg caatttttca gcagccatat tctcccgtaa atgcgcaaca gaagatgtcc
     4981 ctgggatcag caaaatattc ggtgaacgtt gtaacagcca cgccagcgcc acctgcattg
     5041 gtgttgcgcc caggctcgct gctacatccg aaagtgtgga ggattgcagc ggcgtaaagc
     5101 ccccaagcgg gaagaacggc acgtaagcaa tgccaacgcg ggccaaatca tcaatcattg
     5161 cgtcatcagc acgatgagca atgttgtatt cgttctgcac acagacgatt tcggtaatct
     5221 tacgcgcctc tgcgacctgc gtcggtgtga cgttgctcag gccaatatgt tttaccaggc
     5281 cttgttgttg catctctgcc agcacggtca ggctggcttc aatcgatcct tccgccgggc
     5341 catgaccatc ccccatcatg acgcgcaggt taaccacatc cagtacgtcc aggccgagat
     5401 tacgtagatt atcgtgtacc gctttttgca gctctgccgg agaaaatgcg ggcaaccagg
     5461 aagcatcctc accacgtcgt gcaccaattt tagtgacaat tgtcaggtcg tcagagtaag
     5521 gatgtagcgc ttcgcggata atctgattgg tgacgtgcgg accataaaag tcgctggtat
     5581 caatatgatt gacgcccaat gccagcgcct cgcgcagcac ggttatagca acgtggcgat
     5641 ctcttggggg gccaaaaacc ccagggcctg ccagttgcat cgcgccataa ccaagacggt
     5701 taacggattt tgtaccgaga gtaaatgtgt tgctgctcat cgcaccctgc cctgtaataa
     5761 aacggattaa gaaaaatcag tatagaaacg aaaagagata aataatgcga aggcgtaaac
     5821 aagattactg gatgagttgc tctattgtcc ttgaagacac caaaacaaaa cgcctaaagc
     5881 atcgcatccg gcaataaaaa accgcaacct gtatcagcat tgcggtttgt gtatcacgtg
     5941 ctaaaaatta gaacgttttc ttgaagttca gcatcacgcc tttgtcgctg atgccttcct
     6001 ctttccagtt gtacgcatcc agattcagcg aactttcttt gctgctgtac ttcacgccga
     6061 cacttgccag gctgttgatg ccgccgccct tctgaccgtc atcgatgtta aagtgctgac
     6121 tgcctgcacc tgccaggctt gctgtgcgct ggctcttcgc gtagctcaag ttcggcccgc
     6181 cttccagcgt agcgctagcg ctccagccgt ctttacctgc atagtccagt ttcagcccga
     6241 cgatgctgtc caccgccgtt tcgctaccac tgttcatatt caggttaaag tctccggcat
     6301 tgcgctcctg atagccacct tccagtgtgt gacgcagttt cacacccgca tatggtgtga
     6361 ccttcagccc ttcgctcggt tcgaaagtct tcgctccttc gctgcggaac tccaggtact
     6421 gctgtttcac gtcggtatca gcagttttgt tcgtgttgct aaatgcaatc gaacggctgc
     6481 tgtcaaggtt gtgaacgtca taacgcaatg cgttgttcca gttcatgcca ttgtcgaagg
     6541 acatctgatg tttcaggcca aagaactggc tgtaaccgcc tgtaacacca ttatcgccag
     6601 ctttctgcgc accgtcgcca tcgagacgag cgataccgta ctccagactc atggtctgac
     6661 tttcactcag gtcaatggtt ttacgcagtg ccagcatgtc gtactcggta ttgttcccca
     6721 gctccgcgcg cggatcgcct ttcgccacca cgttgaacgc cagaccgtta cccactttcg
     6781 gtgcggcatc tgccagcatg ctaaagcggt tgctgagcac acgcgcttcg cggaataccg
     6841 tggtcgcctg gctaccgctg acctgtttca gggcgctgtt cagttcggca gtcgtgccga
     6901 cgttcaggct ggtatacagc tcgttgttgg tgtaacccgc atccagtgct ttcgcgacgt
     6961 cattcaccga agcatcggtc gccacatcgg tataagcgtt tttgctcata gtgacgtcaa
     7021 cgttgccgct ggcatcggta ctgcctttag cgttccagac aactgaagag gaagtgatat
     7081 tctccgcgcc gctgatattt tcacctttga aaacatcatc aatcaccacg gtagtatcag
     7141 cagttcctgc actaaagcca gtatttacct tgacgttgtc actgataaca aggttattgg
     7201 ctttcaaggt gccggaactt ccgtcgctat tagtaccaat agtgtaattg gtaagttttt
     7261 gctgacttac agcattgact ggtgccgcag gaacattacc ttgcccaggc gtcgcagaag
     7321 cgaccggtac gattatatcc gcagttccat cctgactgtt tctcgtccct gtggtatttg
     7381 gcgcataaat ctcgcaacca atattacaca acaggttgat tatcccatta ttcacaatcg
     7441 ctttagtgga accaccaaac gcccatgaat tatctgcata aacgttaatg acaccgtctt
     7501 tagtattatt aatcgttgtt gcagaaccat ttcctttgat gccaatcaga ccttcaccgt
     7561 tgcttccatc tgctttgccc tgttcagtac ccacgttaat tgtgccgctg ttcactaact
     7621 gaacagtata gctggtggtg cctccgctaa tagcaactga accatcaccc tgaacattaa
     7681 ttgttccggc gttagcaaaa ttatacccgc gacctaaatc aatcagtgga gcatctggat
     7741 tgataccatt aattacaccg gtttctgtat tccaaatcca tttgccgttg ctgttagagg
     7801 ctgtgctggc agttttaatc gcactgtaac cgtttgtggc ggtaattttt ccgcggttaa
     7861 cctgtgaaag agaatcactg gtgcgtgtga tattaacgat ggcattttcc cctcccgcaa
     7921 tagcattgcc gctatttgta agcgttgaac cggcttcatt ataaagtacg aaactgccgt
     7981 taattaaggc actatcatct gtgacagttg tcatctcccc cgtatttaca acgttttgtt
     8041 tataactatc accgctaatt actccgctaa agcttccctc attgaagaag gcagacccgc
     8101 cttttaacat taacagttcg gttgtcatac tgcccgaggc gcgattgtaa acactggcat
     8161 tggcagtaag cttgtcggca tccatgctcc cgctgttttc cagcccctgg acaatattca
     8221 aatcaccatt aactgtcagg ctggccttat cctcgttata taaccatagg ttggcatcga
     8281 catcgccgtt cagtatttct gtaccataat tagccagcgg tttaatggta acaaagccat
     8341 ctcggataac tgtttttgta atgttctctc cactggcact caattcagat aacagttcca
     8401 gattctctgc aggagcacca taatgagagt cggtgctatc aatttccgct ccgttgagat
     8461 taatctcacc atagttgagg ataatgcctg taccatcatt ataaaacgcc tggcccgtat
     8521 caacattaat attgattaca ccagtttggt cgtttattgc cgtagcacct ttatacactg
     8581 ccatccctac cagcgtatta ctacccaggt ttgcatcgta atctgcattt ttctcgaggt
     8641 taatgattcc ctggttaata accatattac tgttaccgta ggcggccata ccggcacctg
     8701 cgttatatac agtaattaca ccaccttcca ggttggtcgc aatagcgttg ttacgagcgc
     8761 cacccccggt gtctgtcccg gttgccatac caacgccata atcttttgcc gttgcccccg
     8821 gaagatcggt atgcaaactg gtggtgtcat tgtcatcaat ccataacgta tccaccgtaa
     8881 tattgcctgc attatccgcc gttgagttat cgatggctaa catgtcaatg ctaccgcgtc
     8941 catacatatc aatgacagca tcttcggcat tgagaacaga gcctttatta tacgcctcca
     9001 tccccatcat gcttgctacg ctgttattaa acacgcgctg atccgttgac cccgtgagtg
     9061 cagccatatt aacaacgcta tgttgctcca ttacttttga ggtaattacc cctttgttta
     9121 ctgcgctgcc gccgttagag gtcaagaagg ccgtgttatc tggttccggt gctggcgtat
     9181 tagttgaggc ataatcatac agagaaagtg tgatattccc gttattgata cctgtggtat
     9241 tttcgccaaa gataccagca aaactgatat tccagatatc aacattaccg ttatttatga
     9301 cctcagagct actttctgcc tttataatct gtggtgtttg tgcatagtaa tgatcataat
     9361 tttcaatatc ccgcatattg aaagtagctt cactgccaat ctcaatagct gctttatttt
     9421 ttaactggat aaggcttgca tatcctagtt gcagatcacc actgatcgtt gagtcaccat
     9481 tcagatatac cttcgattca ccatcaatac caataagtgg cgtattaccg aagaaagtcc
     9541 gtttgttagc aatggctgaa ccatcggtca gcgcatttat ttcaccaaca aatgcaatcc
     9601 cgccattcag attgatgttg ttaccatcac cggaaacaat aagaccagaa atattttcct
     9661 ggctaccatc aaaattcgag acatcacgtg tggtagtttc accatccgca acaagcgtta
     9721 atttaccatc cagtgtcaca ttgttattat gaccactgac atttacgccc acggaagcgt
     9781 cataaaaata ctctgccgca ttgtctgcgc tctgatcttt atcaaccaga atattaccag
     9841 ttatatcaat tgtgttggca tcaccggaga cgttaatacc tgtagctttc tgaccaacga
     9901 cattaagatc ctgcattgcc attgtcaggc tattattatc gccagttaat gtcagccctg
     9961 ttgaaaaatc accgacctgc atacttcccg cgagggagat tttcccctcg tcgctggtaa
    10021 tagcaaatcc cgtcgctgaa ttactgacat ccacatcacc agtattggca agtgtcgcgt
    10081 tgtcgccatt gatcagtaca ccgatcgagt ctgcgtccgt gacggtcata ttgcctttgt
    10141 tatcgacggc ggcattatcg ccagaaatat caatgccgtg accgccgccg ctgacatcca
    10201 gatcaccgtc ctggataact tttccgttat tgccgttgat ttccgtgccg gtggaatcct
    10261 tgccgtcaac ggtagttttg ccgttgttgt tcgccgtggc gtcatcaccg ttaatctgcg
    10321 tgccggtgcc gccgttggtg atggtgctct cgccttcgtt attgacgatg gccttgtcgc
    10381 cgtcgatctg gatccccatg gactccggat cggtgacggt catggtgccc ttgttatcca
    10441 ccgtcgcgct gtcaccggta atatcaatcc cgtgaccgcc accgctgaca tccagatcgc
    10501 cgtcctgaat cacttttccg ttattaccgt tgatttccgt accggtggaa tctttaccgt
    10561 caacggtggt tttgccgttg ttgtttgccg tggcgtcatc accgttaatc tgtgtgccgg
    10621 tgccgccgtt gctgatggtg ctgtcgcctt cgttattgac gatggccttg tcgccgtcga
    10681 tctgaatacc gatggactcc ggatcggtga cagtcatggt acccttgttg tccaccgttg
    10741 cgctgtcacc ggtgatatca atgccgtgac cgccgccgct gacatccaga tcgccgtcct
    10801 ggataacttt tccgttatta ccgttgattt ccgtgccggt ggagtcttta ccgtcaacgg
    10861 tggttttgcc gctgttgttc gccgtggcgt catctccgtt aatctgcgta ccggtgccgc
    10921 cgttgctgat ggaactgtcg ccttcgttat tgacaaccgc cttgtcgccg tcgatctgga
    10981 taccgatgga ctccggatcg gtaacggtca tggtgccctt gttatccacc gtcgcgctgt
    11041 caccggtgat atcaatgccg tggcccccgc cgctgacatc cagatcgccg ccctgaatca
    11101 cttttccgtt attaccgttg atttctgtac cggtggaatc tttaccgtca acggtagttt
    11161 tgccgttgtt gttcgccgtg gcgtcatcac cgttaatctg cgtaccggtg ccgccgttgg
    11221 tgatggtgct ctcgccttcg ttattgacga tggccttgtc gccgtcgatc tggatcccca
    11281 tggactccgg atcggtgacg gtcatggtgc ccttgttgtc caccgttgcg ctgtcgccgg
    11341 taatatcaat cccgtgaccg ccaccgctga catccagatc gccatcctgg ataaccttcc
    11401 cgttattacc gttgatttct gtaccggtgg aatccttgcc gtcaacggtg gttttgccgt
    11461 tgttattcgc cgtggcatca tcatcgttaa tctgtgtgcc ggtgccacca ttgctgatgg
    11521 tgctgtcgcc ttcgttgtta acaaccgcct tgtcgccgtc gatctgaata ccgatggact
    11581 ccggatcggt gacggtcatg gtgcccttgt tatccaccgt cgcgctgtcg ccggtgatat
    11641 caatgccgtg accgccgccg ctgacatcca gatcgccgtc ctggataact tttccgttat
    11701 taccgttgat ttccgtgccg gtggagtctt taccgtcaac ggtggttttg ccattgttgt
    11761 ttactgtggc attatcaccc gttataacaa gctcaccgtt gtgattatat cgccactttg
    11821 ttgtgccatc tgcgctcaca ccttcaaggg caacagtatt attcgcttca tcaatctgcc
    11881 aaagattaat cgttgcctta cgaccactgt tatcttgcag agaaatagtc ccgtcagcat
    11941 tctcggtata agtaaaaact gaatcgcgaa tagttaacgt tttttctgtt ttatcgagaa
    12001 taacgtcatt attaaacgaa atcggtttgg gaggtgtagg cgtatcatcc ggaacgtccg
    12061 gtattatctc atctccgcca ggagttgggg gcactggagt gtcgtctgaa tcatcatcag
    12121 ggggcgtcgg tgggagtgga gaattgttgc gatgatgatg atcatcatcg tcgtttagtt
    12181 ccactacggc taaggtcgtg attaacgcag cagcgcctgt ggcaagccat ggcatcaggt
    12241 tttgttcgac taaaggggtg cattcagagg gtaagcgttt aagctcttcc ggactgagtt
    12301 ttcctggatc tgccggacaa gtcacattag tatttttctt ttcacctgtg gtggtttcta
    12361 cctctgtgat atctgccgcc caaccctgac cactcagagc taatgcaata caggccgaca
    12421 ataaagtttt cctttgcata ataaatcctt ctggcgaata taaagaaagc cggatgcact
    12481 aacccgacca attagttatt taaaacattg aagacatcct atattgccag tggattatta
    12541 tctaatcaaa tatactttac gataattcac agttataatt cagatgaata aaaaaagacc
    12601 atgaaatata tgaattgtgt atattataca tattataaat taaaccaaat taccattata
    12661 aataatcata taatgaaaaa aacggtatga tttaatacta aaataaataa agcagagtta
    12721 tgtttcagct ctgctttata attaaaagga caaaataatt ttacatctgt tactgtttcg
    12781 gtcgcagatt ctcatcaaat accaggcgtt tcctgccagg ttcaacttcc cgcaatggct
    12841 cgacttgatg taacgtctgc ttacagcgtg tcaccagcac ctgatactca tgcgtgccct
    12901 gctttttcca tgccagttcc tgctcactaa gttcacgaat cgcttttgcc gggctgccga
    12961 caatcaggta attagcaggc atttctgctt tggctttcac aaatgcggaa gcgccaacaa
    13021 tgctgttctc gccaatcacc gcaccgtcca tcactaccgc gttcataccc actaatgcat
    13081 tgcggcggat aatgcagccg tgaaggatag cgctatgacc aatatgccca tcttcttcca
    13141 cgacagtatc ctgatcggga aaaccgtgca taacgcaatt atcctgaatg ttcgcgccat
    13201 ctttcaccac gatacgaccg aaatcgccac gcaggctggc atttggccca acgtacacgc
    13261 ccttgccgag aataacatcg ccgatcaata ccgctgtcgg atgaacaaaa ctctcttctg
    13321 gtacaaccgg agtcagaccg tctatctggt aaattggcat aacgcctcct gttcaatgtt
    13381 caaaccgcca aaacgttgaa aatacaggct tcccggcgca ggcagttcac cgaccgaggt
    13441 ttcacctttt tcactaacga acgctaaagc agcaggcgct acgcgctgat aaatgttgat
    13501 acacagctga cgcgccgtat gccctgccca gtgagccgga agcaactcct ccggcaacaa
    13561 tgggtcttta aggacgacac ggcgataaaa atggatcagt aaaagctgaa tatgaaatgc
    13621 acgctccggg gttaattcgt ctgccgcctc ttttaacagc ggcaccagcg ggcggaatga
    13681 ctgaataaag gtttcgtaca tggcattttg ttcagttaaa tgccagcact cttctactct
    13741 ggcacgcagt gctgcgcgag aaagcgccag tggtatttgc gcttcaaaac aaatcacgtt
    13801 atccgccaca cccgcttcat gcaaaagtgt ctgtacatcg gccagttttt gcgacggcga
    13861 tgccatcaga ctgggtgcca gtgcgccaaa accttgccag atcaactgct ttttgacatc
    13921 agccagcgtc gctttatcta aaccttccga gagcaacagg agccatttac catcccatgc
    13981 aggttgctct gcgcgataaa ttttactttc tgcccgtcgc gtcaggcgca agcctttatc
    14041 actgaggcta tagaaactgc gtcgcccgat gcgggaaaca tccagccagc cttctttatt
    14101 aagacgaaac aaagcggtgc ggacgaaacg ctcaccaaat cccagccctt ccagcaaagc
    14161 agccagacta cccagccaga tttcaccacc acgatgggaa agcgaatcac catacagaga
    14221 ggagatcaaa gatgtgccac tgacaggaac agcgtttaca gcatgttgga taaaagtatc
    14281 aagtttactc attcgattca gtctgttgtt attgttgtga tttatgaatc atagcacagg
    14341 tcgtatcgac ttctggccct ggcgctttac ctcaccaggg ccagacgaca catcaggcac
    14401 caacaatatt gcgcagatca aacacccgac acgccttgcc ttctgaacgc gggatactgc
    14461 cacagttcac gatcattaca tcagtagaga tccccaccat cgacttaatc cgatggcgta
    14521 gctggtggca cacctggcaa cgctgctcat gcgtcaatgt caggctgctt tctttcaact
    14581 ccactttcac agaaagtgaa tcaagatgcc cccggcggtt cacctccagt tggtaatgcg
    14641 gcgataaatg ttcgaatttg acaatctctt cttccagttg tgacggaaag acatttacac
    14701 cgcgaataat caacatgtca tcgctgcgtc cgctgatgcg atccatgcgg cgcatagtcc
    14761 gcgccgttcc tggtaacagt cgggttaaat cacgcgtgcg gtaacgaatg accggcaatg
    14821 cttctttggt cagcgtggtg aataacagtt cgccatgttc accatcggca agcggtgtgc
    14881 cgtcctgagg attaacaatt tcaggataga aatgatcttc ccaaatggtt gggccgtcgg
    14941 tagtttccag acactccatc gccacccccg gccccatcac ttctgacagg ccataaatat
    15001 ccagtgcggt gatccccagg cgacgctcaa tttctttacg catggcctgt gtccacggct
    15061 cagcaccaaa tactccaacc cgcagcgagc aaccgctggc atcaccgccc aactgccgct
    15121 ccagctcttc aatcaggtta aggcaataag atggcgtaac catgatcata tctggttgaa
    15181 aatcacggat cagttgcgct tgtttttccg tctgcccgcc agacatcggg atcaccgtag
    15241 cgcccagacg ttcggcacca tagtgcgcac ccagcccgcc agtaaacagg ccgtaaccat
    15301 aggcgacatg aattttgtct ttgggcgagc cccctgccgc acgcaaagaa cgcgctacaa
    15361 tattggccca gttatcaata tcgttttgcg tatagccgac aactgtcggt ttacctgtgg
    15421 ttcccgaaga agcatgaatg cgcaccactt gttccattgg cacggcaaag gtgtcaaagg
    15481 gatagttgtc gcgcagatcc tgtttggtgg tacagggaaa tttacgcagg tctgaaagtt
    15541 ccctgaaatc atcaggatgt acgcctgctg cgtcgaattt gcgccgatac atcggcacat
    15601 tttcatacgc gtgtttgagc gtccatttca gacgctgtgt ttgcaacgcc tgtaactcat
    15661 caacagacgc ggtttcaatc gggtcaagct ttgtattggt tatcattgta ggttactcgc
    15721 aggttgatat gctcaaacac gctccagaat catggcgatg ccctgaccga caccgatgca
    15781 catggtgcat aatgcataac gaccgttacg ccgatgcagc tcatggctgg cagccagtgc
    15841 caggcgggca ccactcattc ccaatggatg gcctaaggcg atagcgcctc cgttggggtt
    15901 aacatgtggg gcatcatcag gcagccccaa ttcgcgtagt acacccaacg cctgggccgc
    15961 gaacgcttcg ttcagttcaa tcacgtccat atcgtgaata ctcaaccccg cgcgttccag
    16021 cacccgacga gttgcaggca ccggaccaag ccccatcagg cgcggttcca ccccggcggt
    16081 tgccatggct acgatacgcg ctcgcggtgt cagtccttgc gctgctgcca tctgttcact
    16141 ggcaataatc aacgcagcgg ctccatcatt caccccggag gcattgcctg cggtgatcac
    16201 tccattggca cgaaatggtg cttttagtcc acgtaactgt tccagcgtcg tttccgggcg
    16261 cagatgctcg tcatgttgta tttctgttac aacacctttt ttgtttttca acacaaccgg
    16321 aacaatctct tcagctaaaa tgcctgaaga ttgcgctttt gctgtacgtt gctgactgcg
    16381 tagcgcaaaa ctatcttgat cttctcgcga gatttttaac agttcagcta cattctctgc
    16441 cgtttccggc atgctgtcag ttccaaattg ttgagccatg agcgggttca caaatcgcca
    16501 gccaatagtg gtatcaaaca tctcagcctg acgagaaaat gcactggttg ccttgcccat
    16561 aacaaacggt gcccgagaca ttgactccac gccaccagcg atcagcaaat cgccatcgcc
    16621 cgctttaatc gcccgtgcgg cgaaccccag tgcgtccagt ccggaaccac acaagcggtt
    16681 aatggttgtg ccggaaacac tctgcggcag ccccgccagt aaagtcgcca tccgggctac
    16741 gttacggtta tcttctcccg cctgattagc acagccgagg atcacatcat cgatacattc
    16801 cgcatcgaga cgcgggtttc gcaccagcag ttcccgcaaa gggatagcag ccagatcatc
    16861 agcccgaaca cctgataatg ccccgccgta gcgaccaatt ggcgtacgaa ttccgtcaca
    16921 gataaaggct tcacgcatca ggcttctcct gtaatggtgc cgccgatgcg gtgagattta
    16981 ccgcgaaaca gcgcaaccgt tttttgttgt tggttaacaa tttcgatgtc gtaaacaccg
    17041 gtttgcttgc cctgatgacg tacctgcgca gtagcagtta aggtgtctcc ggcaaagcct
    17101 ggacgcaaaa aatcaatcgt gcaggcagaa gcgacggctg ccagcccctg gctattgcag
    17161 gcgtaggcaa aggcagtatc agccagtgaa aatagctgcc cgccgtggca actttgatga
    17221 ccattaagca tttgtgcagt gacggtcatg gtcactacag caaagccttc atccattgag
    17281 ataatgtcga tgccaagcgc tttggcgcag gcatcgttct catacattgc atgggcattt
    17341 tgccaggcct tattactcat aaccgctctc cagaagcgct cgttggcgca gcaatgaaca
    17401 tgggcgatag cgttcttcgc catagtgatg ttgtagattt tccaggagcc ttaatattcg
    17461 ctgccagcca agttgcgctc cccaggcaag cgggccatat ggataattca ccccaagacg
    17521 catggcggta tcgatatcct gttcagaggc cacgcctttt tgaagcgcat caagggcttc
    17581 attgatgatc attgctaccg ttcgccaaat cagcattcct gggtaatctg caatttgcag
    17641 cactgttttg ccctgctgtt gcaggtaata aatggccttg cgggtcgctg agtccgggtt
    17701 caccgctgcc gcagcaatgg tcaccacctt gcccgccatt ttgtcgacca ctaccaccgg
    17761 gcgtgccagt cgtattgcca gcgcctgtgc cgtctcgcct tgtgtctcaa tcaataaaac
    17821 atcgtcaatt tccgtgacac cgtcactttt cttttctact ttcatggggc taaaactgtc
    17881 gcttaccgct tccaggccaa caactgcctc acgttccgcg cgccagtcgt acacgcccag
    17941 cccacttttc ttgcccaacc gtccaccaat caccagttcc tgttgcacca gcgaaggtaa
    18001 aaaacgacgc tcctgccaga aagcgttaaa caccgaacag gtgacagcaa aattgacgtc
    18061 ctgaccaatc agatcggtta attccagcgg ccccatcggg aaaccagcgc catcgcgaag
    18121 tgcagcgtca atcacttctg gtgcagcaac ctgctcttcc agtgcccgcc aggcctcgga
    18181 ataataagga cgcgcaacac ggttaacgat aaatccagga gtcgaatgac agcgcacagg
    18241 ctgcttaccc caactcaacg ttagttcaca caactgctca acaacttccg ctgccgttgc
    18301 cagcccactg accacctcca ccaacttcat caccggtgcc gggttaaaaa aatgcagccc
    18361 cgcaacacgt tcaggatttt ttacctccgc agcaatcgcg gttatagaga ttgacgaagt
    18421 gttagtggtc aatagcgttt gtggcgggca aacttccgcc agctgtgcaa agagcgcttt
    18481 tttgacttcc agacgttcag acgccgcttc aatgaccagg tccgcagctg ccagcgcgtg
    18541 aatatcggtc accgggatca ggcgtttcaa tgtgcgttca caggtttcag cagtcagttt
    18601 tccccgcgtc actcgtgaat ttagccgcgc gtgtatcccg tcgattgcgc gggtcagcgc
    18661 ttcagcagaa atgtcataca gtaaaacctg atgtccatga ctggcggcaa cttcagcaat
    18721 gccagccccc atggtgccgc tcccaatcac tgccacagtt tgcacattta tcatcatgct
    18781 atttccccgt gaactgcggt gagcgtttag ccaggaacgc actgacacct tcacgataat
    18841 ctgcgctccg tccggcaagt cgctgatagt cacgttccag atccagttgc gtatcaagcg
    18901 tattggtttc agcgctattt atcgcttgct tgataagtcc aagaccaaat gtcggttgtg
    18961 tcgccagatg ccgtgccagc tgttgcgcgg tatctgccag cgtttcatca tcaacaacct
    19021 gccagatcat cccccattcg tgcgcctgtt cagcactcag ttgattcccc agcagtgcca
    19081 gccccatggc gcgcgctcgt ccggcaacgc gtggcagtaa ccaggtacca ccgcaatcgg
    19141 gtaccaaacc taacttacta aacgccatga cgaattttgc tgaacgggca gcaataacga
    19201 tatcgcaccc cagtgccagt gttgcgcctg cccctgcagc cacgccatta actgcacaga
    19261 tcaccggttt tggcagtttt gccaggcgac gtaccagtgg gttatagaaa cgctccactg
    19321 acatacctaa atccggtgcg gggcctgtgg gatcaacgtt acgatcgtta agatcctgac
    19381 cagcacaaaa tccacgtccg gcacctgtaa ttaacaagca acggatagtg tcgtcgcgct
    19441 cgacctgttt caggcactct gccagttgtg cgtgcatctc atcattaaaa ctgttcaggc
    19501 gttccgggcg gttgagcgtt agtgtcatca cgcccttttc tacatgactg aggatgaatt
    19561 ccatcattag cgtcctttaa agtcgggcgt gcgtttttgt aagaaagcgg agatgccttc
    19621 atgacgatct tctgttgccg ccagcaaggt gaataactgt cgctcctggg caagtccggc
    19681 ttgcaaagcc acttcctgcg actggcgcag cgcttgcttt gccgcttgta aggccagcgg
    19741 cgagtgacgt gccattttcg acgccagctg taaggcgtat tcgagggtta aatcgctggg
    19801 gaagacgtcg ctaaccagcc cggcctgctg tgcttgctga gcggtgatac tttctccgct
    19861 cagcaccatt ttgctggcta acgatttacc gacactacgg attaaacgtt gcgttccgcc
    19921 tgcgccaggc atgatgccga gagtgatttc cggcaaccca aaacgcgcgt tctctccggc
    19981 aaccaccaca tcgcacaaca atgccagttc gcaacccgca ccaagcgcgt aaccgttgac
    20041 ggctgcgatg agtggtttgt taaaggcctg caatcgcgcc catagctgcg gacgtgtatc
    20101 gtttaaggtg gccgcgagat ctttttctgc catttcgttg agatcggccc cagcggcaaa
    20161 aaagcgtgca ttaccggtaa tcacacagac cgaaatgctg ggatcgatag ccgcagcttc
    20221 cagctcattt accagttgcg tcagcaggac attattcagc gcattacgtg cggcaggacg
    20281 gttaagggtc agcaacaata ctcgttgctg acggctgacg atcagttcgc tcatgccatc
    20341 cccttcgcgt caaagtcaac caccacatcg ctggtcagcg gcagtgcctg gcaactcaac
    20401 acatagcctg cagccagttc atccggttcc agactgtaat tggtttccat cgccactttg
    20461 ccacgcagca ctttgcattt gcaggtcgca cagacgccgc ctttgcaggc atagggcaga
    20521 tcagccccct ggcgcaatgc cgcatcgaga atgctttcat cgtcggcatt aagcacgatt
    20581 tcccgatccc gcccatcctg acgcacagtc actttttgtc cgtcactttg cacgttaacg
    20641 ttacgtttga cgcgcgtgcc agccgtatta aaccgctcca gatgaatcgc tttttctggc
    20701 attcccagtg cttttaaggc agcttccgcg tcatccatca tcgccgccgg accacaaata
    20761 aacgcctcat cataaagacg aaaattaatg agcgaggccc caagtgactg taatttttca
    20821 ccgtcaatac gcccgtgaag cagatcgctg tcgagagttt cctgactgaa aatgcacaac
    20881 aactgtaaac gctgaggata tttgtctttc aggtctgcca gtgcctggcg aaacatcatg
    20941 ctctggctgg tacggttacc gtaaatcaga gtgaactgac tttcaggctc ggtttgtaaa
    21001 gtggtggcga taatcgccag cattggcgta atacccgatc ctgctgcaat agccagatag
    21061 cggccctggc gttcggcctg tggctgatag ccgaaatgcc cctgcggcac catgacttcc
    21121 agcgtcatcc cctggcggat gtgttcgcgg gcatagcggg agaaacgtcc gccttcaatg
    21181 gctttcaccg ccacactaat ttcgccaggc aggtagctac ggcaaatgga gtaacaacgg
    21241 cgtaattctt caccattaag gctggctttt aaggtcaaat gttgaccggg acgaaagcga
    21301 tacgcctcct gcaaaggctg gggtaccgca aaggtaatgg ttaccgcatc acgggtttct
    21361 ggctccactt ttgccaccgt taaggaatga aacgttgtca tggcatcctc aaatacattt
    21421 gaaataatcg aaaggttcgc gacagctatc gcagcgatac aatgctttgc aggccgtgga
    21481 accaaattca ctgataagtg tggtatggac gctggcgcaa cgcgggcaac gtacttctgg
    21541 cggcaaatgg gcatggcaac tgtgtccggc gggcgggcta atgccatact gtcgcagacg
    21601 ctcacgggca tcgggggtca tccagtcggt ggtccacgcc gggtcgagtt gcagcacaac
    21661 ctgaacgggg gtaaacccgt tggttgtcat tgcctcacgt atcgcgccaa tcaaatgttc
    21721 cgttgccgga caaccggaat atgtcggcgt aaagccgatc acccatcctt ctcccatctg
    21781 tgtcacatta cgcaccatgc ctaaatcagt aatggtcagc accgggatct ccggatccgg
    21841 gatctggctg agtagcgccc atatctcatg cacctgtggc ggtgcaatgg tggccagacg
    21901 ttgcataccc atctcctctg ttaccattgc tgaccgggta agacacgctg gagatactgc
    21961 atttctgcca ccatgggtcc aaggtgttcg gtatgcagtc cttttttacc gccagtgcga
    22021 tacgcctgct cttgcggtac gttcaatgtg gcttcgttga acccagcaaa aacttcggct
    22081 tcccacgctg cgcgtaaagt gcgtgggtca accgcaatac cctcttcact cagtgcaata
    22141 tcaatctcgt cggcatcgaa cagttcggcg gtaaaacgcc acaacttgtt gatggcctgc
    22201 tgcatctttt gccctgatac gtcagtacca ttgcccagtc gctccagcca gccacgacta
    22261 aaacgcaggt gatagcgagc ttctttaatt gctttggcag aaatcgccgc cagttgcggc
    22321 tcgcggcttt ccatcagacg ggtaaagagc gccacatgcc atgcatcgat gaaatactga
    22381 cgtgcaatgg tgtcggcaaa attgccgttt ggttgttcaa ccaacaataa gttgctgaac
    22441 tggcgctcgt ctcgggtgaa ggccagggta tcttcatcgc cttctcccgc taattcagcg
    22501 gcatacgata agaagttgcg tgcctgacct aataaatcga ggccaatgtt tgccagtgcg
    22561 agatcgattt ccagttccgg tgcgtgaccg caccattcac ccaaccgctg ggagagcacc
    22621 aggcagttat cgcccaggcg caaggtgtaa gccgttaact gattcatttc cgacctcaca
    22681 tgtgctcaat gccatcaggg atggtgtaaa acgttggatg gcgatagacc ttgctttcag
    22741 ccggatcaaa aaattcaccg cgttcttccg gttgcgaggc aacaatttca ctcgccttca
    22801 ccacccaaat tgaacatcct tcgctacgac gggtataagc atcacgggca ttttccagtg
    22861 ccatccgctc atcggcagca tgtaaactgc caacatggcg gtgtgataag ccctgtttgc
    22921 cacgcacgaa aacttcgtat aacggccagt aaacattact cattttgaaa ctccttatgc
    22981 gaccttacag gcatgttgtt tttgtgcatg ggccagcgca gcttcccgta cccaggtacc
    23041 ttcttcccag gctttacgtt tggcgtcgag ccgctcctga ttacaaattc cgcgaccgtt
    23101 tatcacttcg ttaaactcct gccagtcgat ctcaccaaag cggtagtgac cgctttcagt
    23161 atcaaaatgc agatccgggt caggaacggt cataccgagc atttcaacct gtggaacggt
    23221 gttatccacg aaacgctggc ggagttcgtc gttggtgaaa cgtttgattt tccaggcgag
    23281 acttctggcg ctgtttggcg agttatcatc gtttggcccg aacatcatta aggctggcca
    23341 ccagaaacgg ttaatggcgt cttgcaacat ctgcttttga gcttcgctgc cctgcgccag
    23401 tgccatgcag gcttcaaacc cctgacgctg gtgaaaactc tcttctttgc agattttcac
    23461 cattgctctg gcatacgggc cataagaagt gcggcataac gccacctgat tgacgatcgc
    23521 tgcgccatcc accagccagc cgataacacc gatatcagcc caactcaatg tcggataatt
    23581 aaagatggag gaatatttca tccgtccgtc gagcattttt tggtagatgt cttctctggc
    23641 gcagcccagt gtttcggcgg cgctatagag atacagacca tgacctgctt catcctacac
    23701 tttggccaac agaatggctt tacgccgtaa ggttggtgcg cgagtgatcc agttaccttc
    23761 aggcaacata ccaacaatct cggagtgcgc atgctgccca atctggcgga tcaatgtctt
    23821 tcggtaagca tcgggcatcc agtcctgcgg ctcgatagcc gtttcctggg ctatccgttg
    23881 ctcaaagcgt tcttcttggg tcacgtcatc acctttacga ttccagtttt cgtgattcaa
    23941 tcaaaaacac gaatcacatt gttttatgaa agttacacag tggttaacta aaatcacaaa
    24001 ttttgtatat ttgaactgtg acggatttcg caccctattt ttataaatca ttatttatca
    24061 gtgtattaat gtttttcgca gttgctatac agatcgcaca gttaacattt cgttaaaaga
    24121 ccacttgctt ttatgattcg cgatttaact attagcaacg gaaatgtgaa acatctggag
    24181 agtagcgatg cagcagttag ccagtttctt atccggtacc tggcagtctg gccggggccg
    24241 tagccgtttg attcaccacg ctattagcgg cgaggcgtta tgggaagtga ccagtgaagg
    24301 tcttgatatg gcggctgccc gccagtttgc cattgaaaaa ggtgcccccg ccctccgcgc
    24361 gatgaccttt atcgaacgtg cggcgatgct taaagcggtc gctaaacatc tgctgagtga
    24421 aaaagagcgt ttctatgctc tttctgcaca aacaggcgca acgcgggcag atagttgggt
    24481 tgatattgaa ggcggtattg ggacgttatt tacttacgcc agcctcggtg gccgggagct
    24541 gcctgacgat acgctgtggc cggaagatga attgatcccc ttatcgaaag aaggtggatt
    24601 tgccgcgcgc catgtactga cctcgaagtc aggtgtggcg gtgcatatta acgcctttaa
    24661 cttcccctgc tggggaatgc tggaaaagct ggcaccaacg tggctgggcg gaatgccagc
    24721 catcataaaa ccagctaccg cgacggccca actgactcag gcgatggtga aatcaattgt
    24781 cgatagtggt cttgttcccg aaggcgcaat tagtctgatc tgcggtagtg cgggcgacct
    24841 tttggatcat ctggacagcc aggatgtggt gactttcacg gggtccgcga cgaccggaca
    24901 gatgctgcga gttcagccaa atatcgttgc caaatctatc cccttcacta tggaagctga
    24961 ttccctgaac tgctgcgtac tgggcgaaga tgtcacccca gatcaaccgg agtttgcgct
    25021 gtttattcgt gaagttgtgc gtgagatgac cacaaaagcc gggcaaaaat gtacggcaat
    25081 ccggcggatt attgtgccgc aagtattggt taatgctgtc agtgacgctc tggttgcgcg
    25141 attacagaaa gtcgtggtcg gtgatcctgc gcaggaaggc gtgaaaatgg gcgcactggt
    25201 aaatgctgag cagcgtgccg atgtgcagga aaaagtgaac acattgctgg ctgcaggatg
    25261 cgagattcgc ctcggtggtc aggcggattt atctgctgcg ggtgccttct tcccgccaac
    25321 cttattgtac tgtccgcagc cggatgaaac accggcggta catgcaacag aagcctttgg
    25381 ccctgtcgca acgctaatgc cagcacaaaa ccaacaacat gctctgcaac tggcttgtgc
    25441 aggcggcggt agccttgcgg gaacgctggt gacggctgat ccgcaaatag cgcgtcagtt
    25501 tattgccgac gcggcacgta cgcatgggcg aattcagatc ctcaatgaag aatcggcaaa
    25561 agaatccacc gggcatggct ccccactgcc acaactggta catggtgggc ctggtcgcgc
    25621 aggaggcggt gaagaattag gtggtttacg ggcggtgaaa cattacatgc agcgaaccgc
    25681 tgttcagggt agtccgacga tgcttgccgc tatcagtaaa cagtgggtgc gtggtgcgaa
    25741 agtcgaagaa gatcgtattc atccgttccg caaatatttt gaggagctgc aaccaggcga
    25801 cagcctgttg actccccgcc gcacaatgac agaggccgat attgtgaact ttgcttgcct
    25861 cagcggcgat catttctatg cacatatgga taagattgct gctgccgaat ctatttttgg
    25921 tgagcgtgta gtacatgggt attttgtgct ttctgcggct gcgggtctgt ttgtcgatgc
    25981 cggagttggt ccggtcattg ctaactacgg gctggaaaac ttgcgtttta tcgaacccgt
    26041 aaaaccaggc gataccatcc aggtgcgtct cacctgtaag cgcaagacgc tgaaaaaaca
    26101 gcgtagcgca gaagaaaaac caacaggtgt agtagaatgg tcggtagagg tattcaatca
    26161 gcatcaaacc ccggtggcgc tgtattcaat tctgacgctg gtggccaggc agcacggtga
    26221 ttttgtcgat taatcggtca atgaagggca acggcgaata gttgcccttt tatttcacca
    26281 aattttgtga cgttgtcaca ttatgcatga tgtgtatctg tattttcagg gcactcactg
    26341 tatgaaaagc tggcacacct gccaaacaac ctggcaggtg caggcaatcc cctttacatc
    26401 agtactgata atgtgaacct gactaaaccg cccacagagc gcggttgcta acaagaacac
    26461 aacatctgac gaggttaata atgggaagcc cctctctgta ttctgcccgt aaaacagccc
    26521 tggcgttggc agtcgcctta agtttcgcct ggcaagcgcc ggtatttgcc cacggtggtg
    26581 aagcgcatat ggtgccaatg gataaaacgc ttaaagaatt tggtgccgat gtgcagtggg
    26641 acgactacgc ccagatcttt accctgatta aagatggcgc gtacgtgaaa gtgaagcctg
    26701 gtgcgcaaac agcaattgtt aatggtcagc ctctggcact gcaagtcccg gtagtgatga
    26761 aagacaataa agcctgggtt tctgacacct ttattaacga tgttttccag tccgggctgg
    26821 atcaaacctt tcaggtagaa aagcgccctc acccacttaa tgcgctaact gcggacgaaa
    26881 ttaaacaggc cgttgaaatt gttaaagctt ccgcggactt caaacccaat acccgtttta
    26941 ctgagatctc cctgctaccg ccagataaag aggctgtctg ggcgtttgcg ctggaaaaca
    27001 aaccggttga ccagccgcgc aaagccgacg tcattatgct cgacggcaaa catatcatcg
    27061 aagcggtagt ggatctgcaa aacaacaaat tgctatcctg gcaacccatt aaagacgccc
    27121 acggaatggt gttgctggat gatttcgcca gtgtgcagaa cattattaac aacagtgaag
    27181 aatttgccgc tgccgtgaag aagcgcggta ttactgatac gaagaaagtg attaccacgc
    27241 cgctgaccgt aggttatttc gatggtaaag atggcctgaa acaagatgcc cggttgctca
    27301 aagtcatcag ctatcttgat gtcggtgatg gcaactactg ggcacatccc atcgaaaacc
    27361 tggtggcggt cgttgattta gaacagaaaa aaatcgttaa gattgaagaa ggtccggtag
    27421 ttccggtgcc aatgaccgca cgcccatttg atggccgtga ccgtgttgct ccggcagtta
    27481 agcctatgca aatcatcgag cctgaaggta aaaattacac cattactggc gatatgattc
    27541 actggcgtaa ctgggatttt cacctcagca tgaattctcg cgtcgggccg atgatctcca
    27601 ccgtgactta taacgacaat ggcaccaaac gcaaagtcat gtacgaaggt tctctcggcg
    27661 gcatgattgt accttacggt gatcctgata ttggctggta ctttaaagcg tatctggact
    27721 ctggtgacta cggtatgggc acgctaacct cgccaattgc ccgtggtaaa gatgcaccgt
    27781 ctaacgcagt gctccttaat gaaaccattg ccgactacac tggcgtgccg atggagatcc
    27841 ctcgcgctat cgcggtattt gaacgttatg ccgggcctga gtataagcat caggaaatgg
    27901 gccagcccaa cgtcagtacc gaacgccggg agttggtggt gcgctggatc agtacagtgg
    27961 gtaactatga ctacattttt gactggatct tccatgaaaa cggcactatt ggcattgatg
    28021 ccggtgctac gggcatcgaa gcggtgaaag gtgttaaagc gaaaaccatg cacgatgaga
    28081 cggcgaaaga tgacacgcgc tacggcacgc ttatcgatca caatatcgtg ggtactacac
    28141 accagcatat ttataatttc cgcctcgatc tggatgtaga tggcgagaat aacagcctgg
    28201 tggcgatgga cccggtggta aaaccgaata ctgccggtgg cccacgcacc agtaccatgc
    28261 aagttaatca gtacaacatc ggcaatgaac aggatgccgc gcagaagttt gatccgggca
    28321 cgattcgtct gttgagtaac ccgaacaaag agaaccgcat gggcaatcca gtttcctatc
    28381 aaattattcc ttatgcaggt ggtactcacc cggtagcaaa aggtgcccag ttcgccccgg
    28441 acgagtggat ctatcatcgt ttaagcttta tggacaagca gctttgggta acgcgttatc
    28501 accctggcga gcgtttcccg gaaggcaaat atccgaaccg ttcctctcat gacaccggtc
    28561 ttggacaata cagtaaggat aacgagtcgc tggacaacac cgacgccgtt atctggatga
    28621 ctaccggcac cacacatgtg gcccgtgccg aagagtggcc gattatgccg accgaatggg
    28681 tacatactct gcttaaacca tggaacttct ttgacgaaac gccaacgcta ggggcgataa
    28741 agaaagataa gtgattgttt cagactaaaa aaacggcacc tggtgccgtt ttttacttat
    28801 gcgcgaacca gattaatacc gtacacacac cgacttggtt tcacaccaac cgtccagcca
    28861 gtccgggcca aaatcacggc ctgttcctga ctgcttcatc ccaccaaacg gtaagttagc
    28921 gtcaattaag gtatgactgt ttacccacac cgtacctgcc tgtaagcgat cgctatattc
    28981 cagagcctgg gagagatttt gcgtccagac gctggcagtt aagccatatt ccgtgtcgtt
    29041 tgccagttgt aacgcctctt ctccatccgc tacacgcacc aggttaacca ccggaccaaa
    29101 cacctcttca cgagttaagc gcaatttagc attgggattt accaccagcg ttggcgcaac
    29161 ataatatccc tctccggcag gtccactcgc cccgcgaatc agctctgctt gctgtgcctg
    29221 cgcatcgtca aggaatgaac gcactttgtc gcaatgcgca cgagaaacca aagggttaat
    29281 ctgtgcaaca ggtgacatcc ccggtcccac ttgcaacgat tttaccgcct gctcaaatcc
    29341 actaaccagc gtgtcaaaca acggcgcttc aatataaatt cgcgaactgg cggcgcatac
    29401 ttgcccctga ttcaggaaac ttccggtcat caagccttca ataacccatt gcggatcagc
    29461 atcttttaat acaattgccg ggtttttacc gcccagttcc agcgttacac gcgtcaagcg
    29521 atcagcagca gttctggcaa tacctttgcc cgtcgcggtt gaaccggtaa aactgatttt
    29581 cgcaacatga ggatgtgacg tcagggccgc gccgcataca gaacctgacc cggtgaccac
    29641 attaaaaacg ccatcaggaa tacctgcctc gctggccagt tccgccacgc gcaacatagt
    29701 cagtggcgtg gtttccgatg gcttaatcac gattgaacag cctgctgcca gtgctggcat
    29761 caccttccac atgccaatca tcaacggaaa gttccatggc acaattcccg ctaccacgcc
    29821 aaccggctct ttacgcgtcc aggcctgata acgcgccccc tggggtaagg gaatcgacaa
    29881 gtccagcgtt ttacccgcga ttttggtcgt taacccggcg gtataacgca tccagttcag
    29941 cgtacagccc acttcaaaag cacgggaaat agcaattgac ttaccttgct ccagggtttc
    30001 cagctgtgcc agctcctcac cgtgctgttc caccagatcg gcaaaacgta gcaggatacg
    30061 ttcacgctct gcgggtaatc gcccggccca gcgacgcgag acaaaggccc gccaggcaga
    30121 catgaccgcg ttatctacat ccgcttcgtt ggcatcaaca gttgacgcaa tttcttgccc
    30181 ggtggccgga tcaaagatcg ccaaccgttt ttcactttgt gcggggccag gacgaccatc
    30241 aatataaaga ccgtgttgac gatcgagaaa ctgttggacc tggcttaata ctgctacatg
    30301 cggctctgtc atcacttttc cttattattt acccagtgtg atttcagtgt acgcagcgac
    30361 aagaaaagtc acttttttcg ctgtgacatt cgctttgtca gctgtgacaa gctccgcaaa
    30421 tcgtgacaat aaccgcttat gtctctatgc ctgacaagac ttatttccta tagtcaggct
    30481 cattagcttc gtttattgca acacaaatgc aacaataaaa atacatttca cagagcgaaa
    30541 acgtgccatg aaccccgcag tggataatga gtttcagcaa tggctttccc aaatcaatca
    30601 ggtatgcgga aattttaccg gacgcctgct gactgagcgt tacacgggtg tactggacac
    30661 gcattttgcc aaaggactaa agctgagtac cgtgacaacc agcggggtga atttatcccg
    30721 cacctggcag gaagtaaaag gcagcgacga tgcctggttt tacaccgttt ttcagcttag
    30781 tggtcaggca ataatggagc aggatgagcg tcaggtgcag attggcgctg gcgatattac
    30841 gttactcgat gcctcacgcc cctgttcgct ttactggcag gagtcttcta aacagatttc
    30901 attactttta ccacgcactc tgctggaaca atattttccc catcaaaaac ctgtctgcgc
    30961 agaaagactg gacgctgact tacctatggt gcaactcagt catcgcctgt tgcaggagag
    31021 catgaataac ccggcacttt ctgaaacaga aagtgaagct gcgctacagg cgatggtgtg
    31081 tctgcttcgc ccggtacttc atcagcggga atctgttcaa cctcgccgtg aacgtcagtt
    31141 tcaaaaagtg gttacgttga tagacgataa tattcgcgaa gagatattac gcccggagtg
    31201 gatagccgga gagacaggta tgtcagtacg tagtttgtac cgaatgtttg ccgataaagg
    31261 tttggtggtc gcgcaatata ttcgtaaccg tcgtctcgat ttttgtgctg atgcgattcg
    31321 ccatgccgca gatgatgaaa aactggcagg tattggcttt cattggggat tttccgatca
    31381 gagtcatttt tcaacggtat ttaagcaacg ctttgggatg acgccaggcg agtatcgacg
    31441 taaattccgc taactaattc gcgttgcatg tacagcaacg cgaattagtt aaaacagata
    31501 tttgactatg catttttcag ggcgaaaggc aatttgacag gaatttttga atattcacct
    31561 tgtttccgga aaatagaata atcgccttct cctgtagcgt tatcccgata ttgcggtttt
    31621 gctattaaaa ttttcgcacc catttaccgt ttatcccttg tacatattcc cccggtttag
    31681 cccgagctac gagcttttgc ccggcgagtt tcgcgatatc atctaccgag acattatttt
    31741 gctttgccag ttgttggtaa ctcgctttac gttcggcatt aatgtcgttt actaatttct
    31801 ctgtttcagc atctgttttc agcgcaacca gataaccata aaatgtttca cctacccgcc
    31861 cctgagttct ggcttcatcc agagtcaatg ccattacatt gctgcttacc agcccaacaa
    31921 gaaacgcaca aagaagtaat gttttcttca tcatcgcctc agaaaagatc gctacgagtt
    31981 tcaagcagct cttcgacatc tttgtctgcc ttgatgatga tctcatgctc aattttaacg
    32041 ttcatattga tagtgatcgg ttccttaggt gcagcgactt caatgcgagg cgtacagcca
    32101 accagcataa aagatgacgt caacgcagcc agtaaaattt tcattgtttt tcctcacact
    32161 cttttccttg cggacagtca tttcccggca gacgtgcgtt ctgctccaac catgcctgga
    32221 gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat attctcttca tgatggtaat
    32281 taaggtttac cgtaccgctt ttaccatcaa cgcgactggt acctgtaatg ttggcctgca
    32341 tggttaataa accgagatta tctaaattaa tatttgtcga tgaacggcta atttccatat
    32401 agcgcaacca gttaattgct gaacccgcag tcatattgtc tttcaccacc gcatctgcgg
    32461 tgtctttatc cagccgcaag gtcattggcc cgctatttgc cagccagcca tctttcacta
    32521 tccatttttc gttgtttagc cataacggca atgcaccact aaatgccccg gacatggcga
    32581 attgtttcgg attgacggcg ctaaccagtt cgctggatga gagattattc agccgcaaca
    32641 gcgccgggtc atgttgcggc atacgtaatt gtttcatcac cacgttaccg cctaacacat
    32701 cgacgctaac atcagtcaac agcaatggtt cttcttctgt ccacggatag cgcccttgca
    32761 aatcagccgt aatatttttc gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa
    32821 cggggccgcg agtccccaga tgccaggctc catcggcaaa acggaaaggc aggacaaaat
    32881 cgacaccgtt aacctgatta tctggcatcc aggcactacc gcctttcaac acaccgtgcc
    32941 ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc gacctgagca tacagttcgc
    33001 catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg ctggaatacg gtcagtgatt
    33061 gtttaggcca ccaggcgtta ccgcgcagac gaataccgtc ccagcgacca tttacccgaa
    33121 ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgtc
    33181 catcgacgct aaattttaac gttgatggcg gtaacacact gccgccagtg aacagcgttt
    33241 gcccggcgtc cagtgacagc gcgccgctga aggagggatg ctgtgcgtca cgtacccaga
    33301 cgacgggctt gtcgagaatt aatcgcggct tttctaccgt catcgtaccg tattgtaact
    33361 ggtcgaaacc ggtggagaga tcggtcagcg taatcgtgct gtcatgccac tcaccctttc
    33421 ctgcgacatc ccaggtggca ttcatcggtg taaaactgcc ttttccccag tagcgccact
    33481 gccagcggcc agcgtcaggg agaaaatcat tcgccagtcc atccatatgc agcacgaaat
    33541 cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt
    33601 gggtgacttt tacacccgct aaaggccagc ggatttcatc gatgtccagc gaatcgatga
    33661 ctcttccctt cgaacgtaat aacgcacctg gctcaaaggc caacgttggg tcagtcagac
    33721 ttccgcttag ctgcgcaggt aaacgggcat ataaaatgag gtccgcctgt ttcgcttcac
    33781 cggtcagttg cagaggcagc tggctgttat ccatgcttaa ttttcctggg ccaaaattaa
    33841 gcaccgcgtt gcccttaccc gcttgcccct gggtcagcac actcagtcgt ccgctgacca
    33901 gagcgttctc aagccctgcc tgccagttgt cgactttaac acccagtcgg ccactcaaag
    33961 gaaaacctgc atacggccag ctccagcgac catcgcttac ggtcaattgt tgacgagtaa
    34021 tttgccacgg cagatcgagc aacggatcgc cgttatcccg tgccaccaca atcaattgcc
    34081 cgctattttc ctgccagtcc agctcggcat ccaccagtga aggctcctgc gacaagttta
    34141 acgtcgcagt agcatgacca cttacaggaa gtccatccgg cactagcggc atagtaaatt
    34201 cccccaccag ttttaccggc ggctgatttt caaacgcgac gacatccagt tcgctgactg
    34261 taagttgttg ccctttcagc tggccttgaa atttaacttt ttcgccctga taacgcagtt
    34321 gctggatatc ggaggttaat gcgagagaga gttttccctg ccactcctgc cagggagaaa
    34381 aaatcagttt atcgatattg atccaggtgt taggcaacat ggcctgccac tgcgcgaggg
    34441 tttttggagc ggcaagcgat tgctccgtct gcggcaattt caccagacaa gcagaatcaa
    34501 attctaccgt gccgacgttc aataaccagc ggctgggatg tgaaaggctg gcgttggtga
    34561 tatgcgcaag ctgacaatct cccaccagat aacggagatc gggaatgatt aaacctttac
    34621 gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg
    34681 ccagcgtggg aacccacaag ccgagcgtca tcagcagcgt caacggcaca agaataatca
    34741 gtaataacag cgcgagaacg gctttatatt tacccagcat gggtagttaa tatcctgatt
    34801 tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg tttaaccgtt cagttgaagg
    34861 ttgcgcctac actaagcata gttgttgatg aatttttcaa tatcgccata gctttcaatt
    34921 atatttgaaa ttttgtaaaa tatttttagt agcttaaatg tgattcaaca tcactggaga
    34981 aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt acctgcaaca
    35041 ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga cggaaaaaac
    35101 cgctaaaacc gccaatggct gcgaagcggt atgtattttc gtaaacgatg acggcagccg
    35161 cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg
    35221 ttttaataac gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc
    35281 agcctatgat ccagaagccg ttgctgaaca cgccatcggt atgatgatga cgctgaaccg
    35341 ccgtattcat cgcgcgtatc agcgtactcg tgacgccaac ttctctctgg aaggtctgac
    35401 cggctttact atgtatggca aaacggcagg cgttatcggt accggtaaaa tcggtgtggc
    35461 gatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc cgtatccaag
    35521 tgcggcggca ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc
    35581 agacgttatc tctctgcact gcccgctgac accggaaaac taccatctgt tgaacgaagc
    35641 cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg gtgcattgat
    35701 tgattctcag gcagcaattg aagcgctgaa aaatcagaaa attggttcgt tgggtatgga
    35761 cgtgtatgag aacgaacgcg atctgttctt tgaagataaa tccaacgacg taattcagga
    35821 tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc atcaggcatt
    35881 cctgacagca gaagctctga ccagtatttc tcagactacg ctgcaaaact taagcaatct
    35941 ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct tgccgctccc ctgcattcca
    36001 ggggagctga ttcagataat ccccaatgac ctttcatcct ctattcttaa aatagtcctg
    36061 agtcagaaac tgtaattgag aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc
    36121 ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg taaccccaga acagctacag
    36181 cttcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg
    36241 ccagaaatca gctttggtga aaaaatgatg atttccggca gcatgtgtaa ccgctttagc
    36301 ggtgaaggca aactgtctaa tggtaaactg acagccaaag ggctggcaat gacccgtatg
    36361 atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat gctgaaagaa
    36421 ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta
    36481 acttataagc tagcggattt aatgaattaa tagctgccac agctcccggc ggcaagtgac
    36541 tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt accatccagt
    36601 tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc actactggac
    36661 atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac aggttcacta
    36721 ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg catcttttct
    36781 tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca gagggcgacg
    36841 tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat ttttttgccc
    36901 tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga aaatgcagaa
    36961 gaaagccatt tgctaaaatt gaaggattac tactgggcgc gcagcaattt cgtgcgcccc
    37021 tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc gcggttgctt
    37081 cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc tattaccccc agttccacga
    37141 tggcagaaca ggctgatgcc tgggccggaa acggcttgaa aaacgtttgg ggagacacac
    37201 cacgcgtggt tgaaatgcag tcggaagcgg gggctatcgc taccgtgcat ggcgctttgc
    37261 agacgggtgc tctttcaaca tcgtttacgt catcgcaggg tttgctgctg atgatcccga
    37321 cgctgtacaa actggcaggc gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg
    37381 ttgccacaca tgcactctct atttttggcg atcattccga cgttatggcg gtgcgccaga
    37441 cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc gcaagacttt gctctcattt
    37501 cgcacatcgc gacgctgaag agccgcgtgc catttattca tttctttgat ggtttccgca
    37561 cgtcccacga aatcaataaa attgtcccgc tggccgatga cacgattctt gaactgatac
    37621 cgcaggcaga aattgatgcc caccgcgccc gcgggctgaa tccggagcat ccggtgattc
    37681 gcggtacctc tgctaacccg gacacctact tccagtcacg tgaagccacc aacccgtggt
    37741 acaacgcagt ttacgaccat gttgaacagg cgatgaatga tttcgctgcc gcgacaggtc
    37801 gtcagtatca gccgtttgag tattacgggc atccgcaagc ggaacgggtg attatcctga
    37861 tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc cgtggcgaaa
    37921 aagtcggcgt gctgaaagtt cgcctgtacc gtcccttctc cgccaaacac ttgctacaag
    37981 ctctgccggg atccgtacgc agcgtggcgg tactggacag aaccaaagaa cccggtgccc
    38041 aggcagaacc gctctatctg gatgtaatga ccgcactggc agaagccttt aataatggcg
    38101 agcgcgaaac tctgccccgt gtcattggtg ggcgctatgg tctttcatcc aaagaatttg
    38161 gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct
    38221 ttacggttgg tatttacgat gatgtgacca atctgtcact gccgttgccg gaaaacaccc
    38281 tgccaaactc ggcgaaactg gaagccttgt tttatggcct tggtagtgat ggcagcgttt
    38341 ccgcgaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac gcacagggct
    38401 attttgttta cgactccaaa aaggcgggcg gcctgacagt ttctcacctt cgcgtgagcg
    38461 aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc tgccaccagt
    38521 tgcagtttat cgacaaatat cagatggttg agcgtttaaa acctggcggc attttcctgc
    38581 tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaggaa gttcaggccg
    38641 tgctaaacca gaaaacagcg cgcttctatg tgattaacgc agcgaaaatc gcccgcgaat
    38701 gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat ctgacgcaaa
    38761 ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa agttacagta
    38821 gcaaaggcca ggatctggtg gaacgcaact ggcaggctct ggcactggcg cgtgaatccg
    38881 tagaagaagt tccgttgcag ccggttaatc cgcacagcgc caatcgaccg cctgtggttt
    38941 ccgatgccgc tcctgatttc gtgaaaaccg ttaccgcggc gatgctcgcc gggcttggtg
    39001 atgccctccc cgtttcggcg ctgccgccag acggcacctg gccgatgggc accacacgct
    39061 gggaaaaacg caatatcgcc gaagagatcc ccatctggaa agaggaactc tgtacccaat
    39121 gtaaccactg cgtcgccgct tgcccacact cagctattcg cgcaaaagtg gtaccgcctg
    39181 aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc
    39241 gcgggcagaa atatgtcttg caggtggcac cggaagattg caccggctgt aacctgtgcg
    39301 tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagctatc aatatgatgt
    39361 cgcgcctgga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc aacctcccag
    39421 aaatcgaccg tagcaaactg gaacgtattg atattcgtac atcgcagctg attacaccgc
    39481 tgtttgaata ttcaggtgct tgctccggtt gtggcgagac gccgtatatt aaattgctga
    39541 ctcagctgta tggcgaccgg atgttgatcg ctaatgccac tggctgttct tcaatatacg
    39601 gcggtaacct gccctctaca ccgtatacca ccgataccaa cggtcgcggg ccggcatggg
    39661 cgaactcgct gtttgaagat aacgccgaat ttggccttgg tttccgcctg acggtcgatc
    39721 aacaccgtgt ccgagtgctg cgtctgctcg atcaatttgc cgataaaatc ccggcagaat
    39781 tactgactgc gttgaaatca gacgccacgc cagagattcg tcgagaacag gtcgcagctt
    39841 tacgccagca actcaacgat gttgccgaag cacatgaact gctacgtgat gcagatgcac
    39901 tggtggaaaa atcaatctgg ctgattggtg gtgacggctg ggcttacgat atcggctttg
    39961 gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg ctggatacgc
    40021 aatgctattc caataccggt ggtcaggcgt caaaagcgac accgctgggt gcagtaacta
    40081 agtttggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt
    40141 acggccatgt ttatgtggcg cagatttctc tcggcgcgca gctgaaccag acggtgaaag
    40201 caattcagga agcggaagcg tatccgggac catcgctgat catcgcttat agcccgtgtg
    40261 aagagcatgg ttacgatctg gctctcagcc acgaccagat gcgccaactc actgctaccg
    40321 gcttctggcc gctatatcgc ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg
    40381 cgctggattc acgcccgccg tcagaagcac tggaagaaac gttacttcat gagcaacgtt
    40441 tccgtcgtct gaattcgcag cagccagaag tggcagaaca gttatggaaa gatgctgcag
    40501 ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca
    40561 acaccgatta aatgcactgg ataaggatta tccaattcta aaaaaaagcc cggacgactg
    40621 ttcgggcttg tctcattat
//
LOCUS       DAOTRK010000020        39937 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.020, whole genome shotgun sequence.
ACCESSION   DAOTRK010000020 DAOTRK010000000
VERSION     DAOTRK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.020"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            148..489
                     /gene="symE"
                     /locus_tag="QIE41_001120"
     CDS             148..489
                     /gene="symE"
                     /locus_tag="QIE41_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDS3530033.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQKQVREF
                     IGVIAGKQKVA"
     gene            complement(536..2629)
                     /locus_tag="QIE41_001121"
     CDS             complement(536..2629)
                     /locus_tag="QIE41_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDS3530034.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVAFGQQGYSEYFDSFMRHYLTVKTGEIPRTDEV
                     YEAFKLHARSQSVAEKGVDRLVEDIHIYAEYYCAMALGKESDKSLATAFQDLRELKVD
                     VAYPFLLALYHDYKNGDLPHEDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVI
                     IKEKYLESIQVHFLNLPSYRRFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRER
                     VEEFTIEHIMPQNENLSAKWREELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAE
                     KRDIEDGFKHSPLYLNIGLGQCEKWDEAAIRARADRLADLAVQVWQAPSLPEEVLAVY
                     RGQPENKTSYSLSDYPFLADGSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETN
                     FVDVVPQKSRLRLSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMG
                     LIRQAFEKQMENALV"
     gene            complement(2726..2890)
                     /locus_tag="QIE41_001122"
     CDS             complement(2726..2890)
                     /locus_tag="QIE41_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDS3530035.1"
                     /translation="MEFHENKAKAPFIGLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3067..4479
                     /locus_tag="QIE41_001123"
     CDS             3067..4479
                     /locus_tag="QIE41_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDS3530036.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(4722..5678)
                     /locus_tag="QIE41_001124"
                     /pseudo
     CDS             complement(4722..5678)
                     /locus_tag="QIE41_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            6156..7388
                     /gene="mdtM"
                     /locus_tag="QIE41_001125"
     CDS             6156..7388
                     /gene="mdtM"
                     /locus_tag="QIE41_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDS3530037.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPLQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGIIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            7429..8709
                     /locus_tag="QIE41_001126"
     CDS             7429..8709
                     /locus_tag="QIE41_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDS3530038.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADVVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            8825..9976
                     /locus_tag="QIE41_001127"
     CDS             8825..9976
                     /locus_tag="QIE41_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDS3530039.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVEDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTAHVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRHQHNIPYIAIETDYSTSDIGQLSTRVAAFIEML"
     gene            9986..10753
                     /locus_tag="QIE41_001128"
     CDS             9986..10753
                     /locus_tag="QIE41_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530040.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVRPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ALMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRKRR"
     gene            10750..11016
                     /locus_tag="QIE41_001129"
     CDS             10750..11016
                     /locus_tag="QIE41_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDS3530041.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCLPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDSEASTRQ"
     gene            11327..13441
                     /locus_tag="QIE41_001130"
     CDS             11327..13441
                     /locus_tag="QIE41_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3530042.1"
                     /translation="MGSLNIYQDIFPTLGMYSGLKACHEKNNQPFDINTEIETIQKQI
                     NYDINHLNDGFIKRVLNVFIHLISNPDNLELTLNRYSSTTEQIIAKTKRNGLNEFEIN
                     DLKIIFNRQDDNESVLTVNHKDISHSCNVKTAQLQQFIKIMEQKAQLPIYIDKNNLKE
                     SIFSVLRNDPQHVEKEQYLPFDKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPV
                     ENQLTIQDNNNKTETFSLTNLQWENVLQYYRENHQQPNIAGSRNITDNRDKIKNTIST
                     SEIIECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCNTCERFYVAQKSITGDK
                     SERLTRKLSISDAGITMTFTIGDVVINTISTAIPEDSTGQRCIEGLNLAGMDLTGIDL
                     SKMVLRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLEKIDFRKARHLLN
                     INFTNANLRNSNFSGKVLTGVNFTGSDLSNAYLAHIDFTKVIFFPSLIIGAVFDYSNL
                     SDKNLSDKDLTNISCMYTNFSNANLTKCKLFNTNFSAAKFDNTNFTGTEGSNILFNHA
                     WLFNTIFIDTIFKNACFFNAKVNNVSLKKAYLYNDNSNKKANDSTEKQANDSTDKQAN
                     DSIEKQASDSTEKQASDSIEKQANDSTDKQAKNSTDKQAKNSTEQQDSTSLNQARLKK
                     EVNSSFSIPGLTSYQPTYIVDE"
     gene            13697..14539
                     /locus_tag="QIE41_001131"
     CDS             13697..14539
                     /locus_tag="QIE41_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDS3530043.1"
                     /translation="MKKRITFIVFLCAIVAVSLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNTESEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTMTGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            14607..15785
                     /locus_tag="QIE41_001132"
     CDS             14607..15785
                     /locus_tag="QIE41_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3530044.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFTFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHIIAPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            15996..21236
                     /locus_tag="QIE41_001133"
     CDS             15996..21236
                     /locus_tag="QIE41_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HDS3530045.1"
                     /translation="MSSTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSPSPLIENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTLQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTGLGWRHFTPSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSIQTKYALKGYNIEAAALEAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYTQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYTANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDAYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTATVTDFHNNPLKDMKVNFVAPGGSQLDNTSATTDQSGIVRVHLTSSKA
                     GSYSVDASLEADKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNTVILTASVKDV
                     YGHPLPDEDVKFTLPASMTGNFTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAHQQVTFIGDTNSAQLQPLTASLNTIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSGTEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGENITLRILVQDAFNNVIAGQRVRLSAQPTAHITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNAQDELMPGQIITFSVTPEGATLSNTGEILTDQYGQAKVTLTSDKVNVYTVTA
                     TMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTVTSRVEDDYGFPVE
                     GVDVRYTLDTKGRPVVNIPTTRTDQSGQVTATITSTLAETLTVNVQVPGTANQSAAIT
                     LLASTADESKSLLKSDVGTLMADYQHSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIRSIKEMSG
                     HVTANNHTFSTAQFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPGITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTQGAYWTSESDDYNRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(21357..21941)
                     /gene="kptA"
                     /locus_tag="QIE41_001134"
     CDS             complement(21357..21941)
                     /gene="kptA"
                     /locus_tag="QIE41_001134"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDS3530046.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEWPFMPAGVQKQ"
     gene            22191..22874
                     /locus_tag="QIE41_001135"
     CDS             22191..22874
                     /locus_tag="QIE41_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530047.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            22871..23332
                     /locus_tag="QIE41_001136"
     CDS             22871..23332
                     /locus_tag="QIE41_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDS3530048.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            23345..24517
                     /gene="iadA"
                     /locus_tag="QIE41_001137"
     CDS             23345..24517
                     /gene="iadA"
                     /locus_tag="QIE41_001137"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDS3530049.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGVCDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFAHKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            24582..25493
                     /gene="hypT"
                     /locus_tag="QIE41_001138"
                     /gene_synonym="qseD"
     CDS             24582..25493
                     /gene="hypT"
                     /locus_tag="QIE41_001138"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDS3530050.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     KLSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(25486..25878)
                     /gene="iraD"
                     /locus_tag="QIE41_001139"
     CDS             complement(25486..25878)
                     /gene="iraD"
                     /locus_tag="QIE41_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDS3530051.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(25875..25958)
                     /gene="idlP"
                     /locus_tag="QIE41_001140"
     CDS             complement(25875..25958)
                     /gene="idlP"
                     /locus_tag="QIE41_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDS3530052.1"
                     /translation="MENEHQYSGARHSGQAAYVAKRQECAK"
     gene            26550..27380
                     /locus_tag="QIE41_001141"
     CDS             26550..27380
                     /locus_tag="QIE41_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDS3530053.1"
                     /translation="MSMPISNALQSQIITDDHFLHHPKVDNELTRRYEYARMDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTETCSEFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYKVISGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(27521..28294)
                     /gene="uxuR"
                     /locus_tag="QIE41_001142"
     CDS             complement(27521..28294)
                     /gene="uxuR"
                     /locus_tag="QIE41_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDS3530054.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(28509..29969)
                     /locus_tag="QIE41_001143"
     CDS             complement(28509..29969)
                     /locus_tag="QIE41_001143"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDS3530055.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTTAYQQLCERGARECVAAL"
     gene            complement(30050..31234)
                     /gene="uxuA"
                     /locus_tag="QIE41_001144"
     CDS             complement(30050..31234)
                     /gene="uxuA"
                     /locus_tag="QIE41_001144"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDS3530056.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            31574..32917
                     /gene="gntP"
                     /locus_tag="QIE41_001145"
     CDS             31574..32917
                     /gene="gntP"
                     /locus_tag="QIE41_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDS3530057.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPVWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(33090..33992)
                     /gene="fimH"
                     /locus_tag="QIE41_001146"
     CDS             complement(33090..33992)
                     /gene="fimH"
                     /locus_tag="QIE41_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDS3530058.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGFYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(34012..34515)
                     /gene="fimG"
                     /locus_tag="QIE41_001147"
     CDS             complement(34012..34515)
                     /gene="fimG"
                     /locus_tag="QIE41_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDS3530059.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(34528..35058)
                     /gene="fimF"
                     /locus_tag="QIE41_001148"
     CDS             complement(34528..35058)
                     /gene="fimF"
                     /locus_tag="QIE41_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDS3530060.1"
                     /translation="MRNKPFYLLCAFLWLSVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(35068..37704)
                     /gene="fimD"
                     /locus_tag="QIE41_001149"
     CDS             complement(35068..37704)
                     /gene="fimD"
                     /locus_tag="QIE41_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDS3530061.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGSNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(37771..38496)
                     /gene="fimC"
                     /locus_tag="QIE41_001150"
     CDS             complement(37771..38496)
                     /gene="fimC"
                     /locus_tag="QIE41_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDS3530062.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(38533..39072)
                     /locus_tag="QIE41_001151"
     CDS             complement(38533..39072)
                     /locus_tag="QIE41_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDS3530063.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(39136..39684)
                     /gene="fimA"
                     /locus_tag="QIE41_001152"
     CDS             complement(39136..39684)
                     /gene="fimA"
                     /locus_tag="QIE41_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDS3530064.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT        10123 a         9965 c         9581 g        10268 t
ORIGIN      
        1 cgctaatttt catcttattt tcattaattt ttagctggac tttccctgta tttactgatg
       61 atatatccag gtatttagcg cggtgcggat gtgcgccaac acacccacac cgctcatcac
      121 aatccctatt tcacgagaat agtaatcatg actgacacgc attctattgc acaaccgttc
      181 gaagcagaag tctccccggc aaataaccgt caattaaccg tgagttatgc gagccgctac
      241 ccggattaca gccgtattcc cgccatcacc ctgaaaggtc agtggctgga agctgccggt
      301 tttgccaccg gcacggcggt agatgtcaaa gtgatggaag gctgtattgt cctcaccgcc
      361 caaccacccg ccgccgaaga gagcgagcta atgcagtcgc tgcgccaggt gtgcaagctg
      421 tcggcgcgta aacaaaagca ggtgcgggaa tttattgggg tgattgcggg taaacagaaa
      481 gtcgcctgat acattgaccc ggtagcgcca gtgctaccgg gtattattcg ccacgttata
      541 ccaacgcgtt ctccatctgt ttttcaaatg cctgacgaat taatcccata atgtaaggaa
      601 gttgtgcgag gtcgctgaaa ccaatttcca catcaccatt gccccagcgc ccaacatttg
      661 tcacatcttt ggcaatacct ttcggatcga ccagttcatg aaactgcata ttaagcgaca
      721 atcgcagtcg gcttttttgc ggcacaacat caacaaaatt cgtttcagct ttaaacgcaa
      781 tatagagctt caatacttcc tgcgtgatcc ctgcgtccag gcgcataact tcatcgcgaa
      841 gatgatcaaa taacacccgg ctatgcgaac catcagcaag aaaaggataa tcactcaggc
      901 tgtaactggt tttgttttcc ggctgccccc gataaacagc taaaacctct tcaggaagag
      961 aaggcgcttg ccagacctga accgcgagat cggccagacg atcggcacgg gcgcgaatgg
     1021 cggcttcatc ccatttttcg cactgtccaa gaccgatatt caaataaagc gggctatgct
     1081 taaagccgtc ttcaatatcg cgtttttccg cgaaaggtct gtcactgtag cgggagttat
     1141 agcgcgttaa agtgagattc cccaacgtat gtaacagttc tttatgcaca cgctgccagt
     1201 cgcttcccaa ctcttcgcgc catttagccg acagattttc gttctgcggc ataatatgct
     1261 caatcgtaaa ctcttccacg cgctctctgc gtttatcgtt ttccagtcgt cgtaaccagt
     1321 agctgcgact acggaagtta tagagatcgc gaacttttaa ttcccgttta aattcatcat
     1381 cgttggggaa acgacgatat gaaggcagat tcagaaaatg tacctgaata ctttccagat
     1441 atttttcttt aataatgacc ttataaaagg tagcaaacgt cttgttaaga gaatttgtcg
     1501 gaatcgcaca tactgcacgg cggaaaacat aagattcaat taaacgaatt atgctcagga
     1561 aatcttcgtg aggcaaatcg ccatttttat agtcatgata aagcgccagt aagaaaggat
     1621 acgccacatc gacctttaac tcgcgcaaat cctgaaaagc cgtagcaagc gatttgtcac
     1681 tttctttacc caatgccatt gcacagtaat actccgcgta gatgtgaata tcttcaacca
     1741 ggcgatctac gcctttttca gcaacactct ggctgcgggc atggagttta aatgcctcat
     1801 agacttcatc tgtacgaggg atctcccccg tttttaccgt cagataatga cgcataaagc
     1861 tgtcaaaata ttcgctgtaa ccttgctgac caaaagcgac ttccattgga cgccagtgat
     1921 cttcatacaa ccgggtttga tgctctggtt cgaggcccat cagaataaaa ttgcgcacca
     1981 gatcggcctg acttaacgcc ttaccggtgg agttcatact ttcaaaaatc agttgcggat
     2041 tatcctgacc acgattaagc gccacatcga caattaataa ctttgctaac ccacgacata
     2101 aggggatcaa gtcatcaccg agtttggcaa tttgttcatc aaagaaagta aagttttcca
     2161 ttattcgatg ggaatagttt tctggcattg gtctttgttt tattaacgcc agcaaactgt
     2221 ctttatcagt ctcggtcagc agtaatttaa atcctttctc gccggactca taaggattga
     2281 gcaaataata attacgcaat ttcattgccg aaaagccatc aaaaacttcg tcttcgccaa
     2341 gatggcgcga taaagcctca atcagcaaca ttgcggtcgt cagccgctgc tgaccatcaa
     2401 tgaccagcaa cggagaaata ccagaaacct gatacaatcc ctgctctata taaacaaccg
     2461 aaccgataaa atgcgctgat atatcgtcac gctttccggc acgaatgatg tcgtcccaaa
     2521 gttgccgaca ttgttgttct gtccatgaat aagtccgttg ataaatggga ataacaaact
     2581 gctgcgaacg ttttaaaaaa tcgagtaggc gggcttctgt agctttcatt ctgacgtctt
     2641 tccgtgaagc gatgatttcg caataataac cacttccttg tggaggttat agagaaatcc
     2701 tgtttatccg cgacgaacag ccccctcact ccacatcctc cctgcgtaac ccaatatccc
     2761 tcaaccgctc atcactcatc tgctgtaaca cccgccgggc ctgcatttgc cgccgccaac
     2821 gcctcactgc ctgccagagt tgcacaagtc caataaacgg tgctttagct ttgttttcat
     2881 gaaattccat cgactgcctc ctcatctttc agaggcttca tcatccgtct tctggcagtt
     2941 cacaatacag atgcaaatat tagttttatt taacatacag acaggttaaa acagcatctg
     3001 catccggttt tttagcctca tctgtaccgg tttttctctc tgtatggtcc aaagaaggaa
     3061 tacagcatga cgcgttatca acatctggcg actctgcttg ccgagcggat tgagcaaggg
     3121 ctgtatcgtc acggggagaa attgccgtcg gtgcgcagct taagtcagga gcacggcgtc
     3181 agtattagca ccgtgcagca ggcgtaccag acgctggaga cgatgaagct catcactccg
     3241 cagccgcgtt cgggttattt tgtcgcacaa cgtaaagccc agccgccagt gccgccgatg
     3301 acgcgtccgg tgcagcgccc ggtggaaatt acccagtggg atcaggtgct ggatatgctg
     3361 gaggcgcata gcgacagttc cattgttccg ttaagtaaaa gcacgccgga tgtcgaaacg
     3421 cccagcctga aaccgctgtg gcgggagcta agccgggtgg tgcagcataa tctgcaaacc
     3481 gttctcggtt atgacttgtt agccggtcag cgagtgttgc gagagcagat tgcccgcctg
     3541 atgctcgaca gcggctcggt ggtcaccgcc gatgacatca tcattaccag cggctgccat
     3601 aactcgatgt cgctggcgtt aatggcggtg tgtaaaccgg gcgatattgt cgcggtcgaa
     3661 tccccctgtt attacggttc gatgcagatg ctgcgtggca tgggcgtgaa agtgattgaa
     3721 atcccaaccg atccagaaac aggcatcagc gttgaagcgc tggaactggc gctggaacag
     3781 tggccgatta aaggcatcat tctggtgcca aactgtaata atccgctggg atttattatg
     3841 ccggacgcgc gcaaacgggc cgttctctct ctcgctcagc gtcatgatat tgtgattttt
     3901 gaagatgatg tctatggcga actggcaacg gagtatccgc gcccgcggac cattcattcc
     3961 tgggatatcg acgggcgagt gctgttgtgc agctcgttca gtaaaagtat tgcacctggc
     4021 ctgcgtgtgg gctgggtcgc gccgggacgt tatcacgata aactgatgca tatgaaatac
     4081 gccatcagca gctttaatgt accgtccacg caaatggcgg cggcaacgtt tgtgctggaa
     4141 ggtcactatc atcgccatat ccggcggatg cggcagactt atcagcgcaa tttggcgctt
     4201 tatacctgct ggatacggga atattttccc tgcgaaatct gtattacgcg cccgaaaggc
     4261 ggatttttac tgtggataga attgcctgaa caggtcgata tggtctgcgt cgcgcggcag
     4321 ctgtgccgca tgaaaatcca ggtggcggca ggctcgattt tctcagcttc cggcaaatac
     4381 cgtaattgtc tgcgcatcaa ctgcgctttg ccgctcagcg aaacctatcg cgaagcactg
     4441 aagcaaattg gcgaggcagt gtatcgggca atggaatgaa ttcgaaatag tggcgaagcg
     4501 cctgatttac tctcacgaat cgcctgtaac tcgttttatc tttatagcga catcgccacg
     4561 ttctggcatg gctcaggcaa agtgtgcttc gttcatgccg gatgcggcgt gaacgcctta
     4621 ccaggtctac aaaatcttta aagttcaata tattgaatgg gttatgtaga cttgataagc
     4681 gtagcgcacc aggcagcttt gcgtttgtca taagtctctg attagtactc gatccaagga
     4741 tcgcgcttgc tttcaggcaa gggctgctct aatgcctgca tttgctctgc cgagagtccg
     4801 gtaatctttt gtatccattc aggatctgcc ccattctgca acaacaatcg gaggaggctc
     4861 cgttcccctt ctactttccc ttctttcctc cccctctccc ttcccaacag ccatccatca
     4921 ttatgaattc gctctgcaat cgtcattatt cgctcccttt gttgtggcat tcgactggtt
     4981 agttcgctga taaactcttt aaaacgcgct tcatcgccag tcagtaaaat gtaatttaac
     5041 agcgcggtta tctggctgtc attagcacac tctgtaacca gtaatagtac cagttgatcg
     5101 ataagcccca tcagatcgcg ctggcgaata tgcttttgga tcaactccaa cagggcgact
     5161 ctgcgatgct gcacaatctc gtcgtctggc acgacagtga catccaccag cgggaacgct
     5221 gcggtataaa gcttccgtgc ggtggtcggg tcagcaaatt tgtccagcca gcacagggac
     5281 cagggataag gactacggct accgtgataa aacagcatcg ggatgaccag cggtagcggc
     5341 cggcgtttat catgctcgat atggcgctgc atcaccgcca tggaatatcg catcaggcga
     5401 aaggccatat ggatatcctc gcggctctga tgttcaatca ctacgtaaat atagccatcg
     5461 ccttaacggg tctttaccga ccacagaata tcagagtgta gcgcccgcaa tttttcatcg
     5521 acaaaactgg cggattccag ttttaaggtg tcgagatcgc acaattcacg taaatctatt
     5581 ggtaagtgga tctccataaa atcccgcgcg gtgtcagggt gtgtgagaaa agatttaaat
     5641 agtgcatcgt gcggtgtgct ggtcgtgaag tttgtcatgg cgatccgtca cctgttgata
     5701 agatggcccg actttagcga ctaacaaaac cattctcacc cggcaatttc ccattccccg
     5761 aggcgctttc caggctattt ttgttcctct gcaaacgata catttttccg gaaacaggct
     5821 cgcgttttac atttcctgta cccggcagat attccccttt ccaccgcact cttttgatta
     5881 accgcaaatt ttaattacac ttaaggtgta tattttctat gcaacccatc aattcaagag
     5941 gtgtaatgtg ctgatgacta tttgtaatcg ttatacattc tgacccgaag tcagaaggta
     6001 tttctctgtc tgtgtgttca caggcagtgt ggttgattac atgaaattca gtacatttgc
     6061 agtctcgttg cccttctcgc ctgcctttcg tcattaccga cggtattgaa tttcgttttc
     6121 cccgttgggg ttctccggac aaggagttgt ttgttatgcc acgttttttt gctcgccatg
     6181 ccgccacgct gtttttcccg atggcgttga ttttgtatga ctttgctgcg tatctgtcga
     6241 cggacctgat ccagccaggg atcattaatg tggtccgtga ttttaatgcc gatgtcagtc
     6301 tggccccggc tgccgtcagt ctctatctcg ctggaggcat ggcattgcag tggctgctgg
     6361 ggccgctttc cgacagaatt ggccgcaggc cggtgctgat taccggggcg ttaattttta
     6421 cccttgcctg cgccgcgaca atgttcacca cgtcaatgac acaatttctt atcgcgcgtg
     6481 caattcaggg caccagtatc tgttttattg ccaccgttgg ttatgtcacg gtgcaggagg
     6541 cgttcggaca gacaaaaggg atcaagttga tggcgattat cacctccatc gtactgattg
     6601 cgccgattat cggcccgctt tccggcgcag ctctgatgca ctttgtgcac tggaaagtcc
     6661 tttttgccat cattgcggtt atgggtttta tctcatttgt tggcttactg ctggcgatgc
     6721 cagagacggt gaagcgcggc gcggttccat ttagcgccaa aagcgtcttg cgcgattttc
     6781 gtaatgtctt ttgcaatcgg ctgttcctct ttggcgcagc aaccatctct ttaagctata
     6841 tccccatgat gagttgggtg gctgtctcgc cggtgatcct gatcgacgcg ggcggcttaa
     6901 caacttcgca gttcgcctgg acacaggttc cggtgttcgg cgcggtgatt gtcgcgaatg
     6961 ccatcgtggc gcgtttcgtt aaagatccga ccgaaccgcg ctttatctgg cgtgccgtac
     7021 cccttcaact ggttggcctc gcgctgttga ttatcggcaa tctgctgtca ccgcacgtct
     7081 ggctgtggtc ggtgctgggc accagtttgt atgctttcgg gattggtttg atcttcccga
     7141 ccttattccg ctttacgctg ttttccaata acttaccgaa aggaaccgtc tccgcatcgc
     7201 tgaatatggt gatcctgatg gtgatgtctg tctcagtcga aatcggccgc tggctgtggt
     7261 ttaacggcgg tcgcttgccg tttcatctgt tagccgtcgt ggcgggcatt atcgtcgttt
     7321 tcaccctggc gggattgctc aatcgcgtgc gccaacatca ggctgccgag ctggcggagg
     7381 agcggtgatt tttgcgcgat cctaccgtca ggctctattc ttaacgttat gaataaactc
     7441 attgaactca gacgcgccaa aatgttggcg ctctctttat tgcttatcgc cgctgctacc
     7501 tttgtcgtta cgctgttttt gccgcccaat ttttgggtga gcggcgtgaa ggcgattgct
     7561 gaagcggcga tggtcggcgc gctggcggac tggtttgcgg tggtggcgct gtttcgccgc
     7621 gtgccgattc cgatcatttc tcgtcatacg gcgattatcc cgcgtaataa agaccggatt
     7681 ggcgaaaatc tcggtcagtt cgtgcaggaa aaatttctcg atacccaatc gctggtggca
     7741 ttgattcgac gtcacgaacc ggcgttgttg attggcaact ggtttagtca gccagaaaac
     7801 gcccgccgcg ttggtcagca tctgttgcag atcatgagcg gttttcttga actgaccgat
     7861 gatgcgcgta ttcagcgcct gcttaagcgc gcagtccatc gggcgattga taaggtcgat
     7921 ctttccggca ccagtgcgtt gatgctggag agtatgacca aaaacgatcg tcatcaggtg
     7981 ctgctggata cgctgatcgc acagttgatc gcccttctcc agcgcgataa atcgcgcaag
     8041 tttatcgccc agcagattgt tcgctggctg gagagtgagc atccactgaa agccaaaatt
     8101 ctccccactg aatggctggg cgaacatagc gcggagttgg tttctgacgc ggtgaattct
     8161 ttgcttgatg atatcagccg cgatcgtgcg catcagatcc gccatgcgtt tgatcgcgcc
     8221 acctttgccc tgatcgacaa gctgaaaaac gatcctgaaa tggcagcgcg agccgatgtc
     8281 gtaaaaagct atctgaaaga agacgaagct tttaaccgct atctcagtga attgtggggg
     8341 gatttgcggg aatggctgaa agcggacatc aacagtgaag attctcgcgt gaaagaacgt
     8401 atcgcgcggg cggggcaatg gtttggcgaa acgttaattg ccgatgatgc cttgcgggcg
     8461 tcgttaaatg gtcatctgga acaagccgcg caccgcgtcg cgcctgagtt ttccgcattc
     8521 ctgacgcgac atatcagcga tacggtaaaa agctgggatg cgcaggatat gtcgcggcaa
     8581 atcgagttaa atatcggcaa agatctgcag tttatccgtg tcaacggtac gctggttggc
     8641 ggttgtattg ggctaatttt gtatttgctt tcgcagctcc cggccttgtt ccccctcggc
     8701 aatttttaga aatccatgaa aaactaagtg gctaaccgcg agagagatca aataaaccac
     8761 actaaaagaa tggtaataat gtggcctctt ttcattatct cccgtggtac ggggaaggaa
     8821 aatcatgtca cttgtcaccg atctacccgc catttttgat cagttctctg aagctcgcca
     8881 gaaaggcttt ctcaccgtca tggatctcaa ggagcgcggc attccgctgg ttggcaccta
     8941 ctgcaccttt atgccgcaag agatcccgat ggcagccggt gcagttgtgg tttcgctctg
     9001 ttccacctct gatgaaacca ttgaagaagc ggagaaagat ctgccgcgca acctctgccc
     9061 gctgattaaa agcagctacg gcttcggcaa aaccgataaa tgcccctact tctacttttc
     9121 tgatctggtg gtcggtgaaa ccacctgcga cggcaaaaag aaaatgtatg aatacatggc
     9181 ggagtttaag cccgttcatg tgatgcagtt gccaaacagc gttgaggacg atgcctcacg
     9241 cgcgttatgg aaagccgaga tgctgcgctt gcaaaaaacg gtagaagaac gttttgggca
     9301 cgaaattagc gaagatgctc tgcgcgatgc cattgcgctg aaaaaccgcg aacgtcgcgc
     9361 actggctaat ttttatcatc ttgggcagtt aaatcctccg gcgcttagcg gcagcgacat
     9421 tctgaaagtg gtttacggcg caaccttccg gttcgataaa gaggcgttga tcaatgaact
     9481 ggatgcaatg accgcccacg ttcgtcagca gtgggaagaa ggccagcgac tggacccgcg
     9541 tccgcgcatt ttaatcaccg gctgcccgat tggcggcgca gcagaaaaag tggtgcgcgc
     9601 gattgaagag aatggcggct gggttgtcgg ttatgaaaac tgcaccgggg cgaaagcgac
     9661 cgagcaatgc gtggcagaaa cgggcgatgt ctacgacgcg ctggcggata aatatctggc
     9721 gattggctgc tcctgtgttt cgccgaacga tcagcgcctg caaatgctca gccagatggt
     9781 ggaagaatat caggtcgatg gcgtagttga tgtgattttg caggcgtgcc atacctacgc
     9841 ggtggaatcg ctggcgatta aacgtcatgt gcgtcatcag cacaacattc cttatatcgc
     9901 tattgaaaca gactactcca cctcagatat cgggcagctc agcacccgtg tcgcggcctt
     9961 tattgagatg ctgtaaggag tggcagtgac atattcgatt ggcattgatt ctggttctac
    10021 agccaccaaa gggatcttac tggcagacgg cgtgattacg cgccgtttcc tcgttccaac
    10081 cccctttcgc ccggcaacag caattactga agcctgggaa actctgcgcg aagggttaga
    10141 aacaacgccg tttctgacgc tcaccggcta cggacggcaa ctggtggatt ttgccgataa
    10201 acaggtgacg gaaatctcct gtcacgggct gggcgcacgg tttcttgcgc cagcaacgcg
    10261 cgcggtaatc gacatcggtg gtcaggacag caaagtgatt cagcttgatg atgacggtaa
    10321 cctgtgcgat ttcctgatga atgacaaatg cgcggcgggc accgggcgtt tcctggaagt
    10381 gatctcgcgc acgcttggca ccagcgtcga gcaactcgac agcattactg aaaatgtcag
    10441 accgcacgcc atcacgagta tgtgcacagt gtttgccgaa tcagaagtga tcagcctgcg
    10501 ctcagcgggc gtcgcgccag aagcgattct cgccggagtg attaacgcga tggcgcggcg
    10561 gagtgccaat ttcattgctc gtctctcctg tgaagcgccg attctgttta ctggtggcgt
    10621 tagtcattgc cagacgtttg ccctgatgct ggaatctcat ctgggaatgc cggtaaatac
    10681 ccatcctgat gcgcaatttg ctggcgcaat tggcgcagcg gtaattggtc aacgagtgag
    10741 aaaacgccga tgaaagagtt tttattttta tttcactcca cggtcggcgt catacaaacc
    10801 cgcaaggcgt tgcaggcagc gggcatgact tttcgcgtca gcgatattcc ccgtgattta
    10861 cgcggcggct gcgggttatg catttggctg acctgcctgc ccggcgagga aatacaatgg
    10921 gtgatccctg ggcagaccga agcgatatat tgccagcagg gcagcgactg gcagtgcgtg
    10981 gtgcattacg attcagaagc gtcaaccagg caataagacc tttaaatata acgcccggat
    11041 atttggctgc acataatcct gcatgaagga agttgtatta aatatctcgc ctgatgtgaa
    11101 atatttccca tccacaaaat gagcgatttt tgatagcacc ttactattgc gcgtctaaaa
    11161 tacaatatac attgtattat acttaaacca taattataca taaagaaata aaatgattac
    11221 cgggtgtggt tatgataaag ataagtttca gcgtgaagct tatctttacc catccccctg
    11281 attattttag atttagaaga acatttcaca attcaaggat aaaaatatgg gctccctaaa
    11341 catataccag gatattttcc caacactcgg catgtattcg ggactaaaag cttgccatga
    11401 aaaaaacaac caaccatttg atattaacac ggaaattgaa accatacaaa aacaaattaa
    11461 ttatgatata aatcatttga atgatggttt tattaagcgt gtactgaatg tttttattca
    11521 ccttatctct aatcccgaca atctcgaatt aaccttaaat cgatattcat caacaacaga
    11581 acaaatcatc gccaaaacca aaagaaatgg tttaaatgag tttgaaatta acgatctaaa
    11641 aataatattt aatcgacaag atgataatga aagcgtatta actgttaacc acaaagatat
    11701 aagtcatagt tgcaatgtta aaaccgcgca actgcagcag tttattaaaa taatggaaca
    11761 aaaagcgcaa ctaccaatct atattgacaa gaacaatttg aaagagagta ttttctctgt
    11821 tttgcgaaat gacccacagc atgtagaaaa agagcaatat cttccctttg ataagttttt
    11881 aaaacatgcc tgcaaaagtt caaattcttt tgaagtgaaa ttagatgcca ctcatcaata
    11941 tcaacacctg aataacttca tgatttcttt tgacccagta gaaaatcaat taacaataca
    12001 ggataacaat aacaagactg aaactttctc gttgacaaac ttacaatggg aaaatgtgct
    12061 gcaatactat agagaaaacc accagcagcc aaatatagca ggatcacgaa atatcacgga
    12121 taatagagat aaaattaaaa atacaatatc cacctctgaa attattgagt gcgcctctcc
    12181 tgaaataaga agtagcgtcc tgaacgatct ttatagcatt gctaatttcc tcccggacaa
    12241 taatctgacc ccaaatgaga gctggaaaag attttgtaac acatgcgagc gcttttacgt
    12301 tgctcagaag agtatcactg gagataagag tgaacgtctt acacgaaaac tctctatctc
    12361 tgatgcagga attacaatga ccttcacgat aggtgatgtt gtcatcaata ctattagcac
    12421 tgctattcct gaagattcaa cggggcaacg gtgtatcgaa gggttgaatt tagcgggaat
    12481 ggatttaacc ggcatagact tgtctaaaat ggttctaagg aatgtcaatt ttaatggcag
    12541 cattcttaga aatgccaagt tctccggtac gatctgtgaa ggcgtggatt ttaccgattg
    12601 tgatctgcgt aatgcagaat tcgaaaatgc ctcattagaa aaaatcgatt ttcgtaaagc
    12661 tcgtcacttg cttaatataa attttacaaa tgcaaatcta cggaacagta acttcagcgg
    12721 aaaagttctc actggcgtta attttactgg aagtgacctt agtaacgctt atcttgcaca
    12781 catagatttc acaaaagtga ttttctttcc gtcattaatt attggagcag tatttgatta
    12841 ttctaatcta tcagataaaa atctttcaga caaagatctt actaatatta gctgcatgta
    12901 taccaatttt tcaaacgcta atttaacaaa atgcaaactc tttaatacaa acttttcggc
    12961 tgcaaaattt gataatacta atttcactgg tacagaaggt tcgaatattc tatttaacca
    13021 tgcatggttg ttcaatacga tatttataga tacgattttt aaaaatgcct gttttttcaa
    13081 tgccaaagtg aataatgttt ctcttaagaa agcatatctt tacaatgata atagcaataa
    13141 aaaagccaat gacagtaccg agaaacaagc caatgacagt accgataaac aagccaatga
    13201 cagtatcgag aaacaagcca gtgacagtac cgagaaacaa gccagtgaca gtatcgagaa
    13261 acaagccaat gacagtactg ataaacaagc caagaacagt accgataaac aagccaagaa
    13321 cagtaccgag caacaggaca gtaccagttt aaatcaagcc cgtttaaaga aagaagtgaa
    13381 tagcagtttt tccattccgg gtttaacgtc ttatcagcca acgtatatag ttgacgaata
    13441 gttcaataat cacccaaaca gagtgagtat ggcagaggat ataaattgct aaccgcctca
    13501 tctatcattc atggatgagg cgcatttatc agtcgatgta agctgaaaat gttccgccat
    13561 ccagagacaa ccgccgcccc ctgcggcgtt tactatttca tcgcccgcgt taagaattca
    13621 ttaagcttca tccgtatact ccttatctgt cgcactgaat ttgacgagaa aaaaagcaat
    13681 aataaggagc aacaagttga aaaagcgcat cacctttatc gtttttcttt gcgccatcgt
    13741 ggcggtgagc ctgttttttg tgcaatcctg cgtccgtaaa agccagcatg tcgcagggtt
    13801 tcagaattat caggccacta tcgatggtaa agagattaca ggagtaacta aaaatatctc
    13861 ctcactgacc tggtcagcgc agagcaatac cttgtttagc actatcaaca aacctgcaac
    13921 catcgttgaa atgacgaccg agggtgacct gatccgcacc attccgctcg attttgttaa
    13981 ggatctcgag acgattgaat acattggtga taataaattt gtcatcagtg atgaacgtga
    14041 ctacgccatt tatgtgattt cacttaacac tgagtcggaa gtaagcatcc tcaaaaaaat
    14101 taaaatccca ttgcaggaaa caccaactaa ctgtggcttt gaagggctgg cttactcctc
    14161 tcaggaccat accttctggt tttttaaaga gaaaaatccg atagaggtct acaaagtaac
    14221 cgggctattg cgtagcgatg agttgcatat cagcaaagat aaagcgttgc agcgtcagtt
    14281 tactttggat gatgtttctg gcgcggaatt taacccacag aaaaatacgt tgctggtgtt
    14341 gtcacatgag tcacgcgccc tacaggaagt gaccatgaca ggtgacgtga tcggcgaaat
    14401 gtcgttaacc aaaggcaagt atggcctttc acacaacatc aagcaggcgg aagggatagc
    14461 gatggatgac agcggcaaca tctatatcgt tggcgaaccg aatctttttt atcgctttac
    14521 gtcgacaaaa tcacgataac agtctggcag gttctccttt tttgttatta acttgttaac
    14581 tcaaaataat acaaaccagg cagaacatga attcgacatc gcatcccgta gaacgttttt
    14641 ctttcagcac cgcgttattc gggatgctgg ttctgacctt aggtatgggt ttaggccgct
    14701 ttctctatac gccgatgctg ccagtcatgc tggcggaagg cgaatttacc tttagcgaac
    14761 tctcgtggat cgccagcggt aactatgccg gatatctggc ggggagcctg ctgttttcat
    14821 tcggcgcgtt tcatttaccc tcacgcctgc gcccgttctt gttagcttcc gctctcgcaa
    14881 ccggattatt aatcctcgcg atggcgtggc tgccgccgtt tcttctggtt ttcatcattc
    14941 gctttctggc gggggtcgcc agcgccggga tgttgatttt cggctcaacg ctcatcatgc
    15001 aacatacccg ccatcccttt gtccttgcgg cgctattttc tggtgttggc gtcggcatcg
    15061 ctctgggcaa tgaatatgtg ctggcaggcc tgcattttgc cctctcttca caaacgttgt
    15121 ggcaaggtgc cggagcactt tctgccatta tattgcttgc tctggcgctg ctcatcccgt
    15181 cgaataaaca cattatcgcg ccagcgccat tggcaaaaat cgcgcaacaa cccatgagct
    15241 ggtggttact ggcgattttg tatggtctgg cgggttttgg ttatatcatc gtcgccacct
    15301 acctgccgct catggcgaaa gacgcgggcc agcctgtgct gacggctcat ctttggacac
    15361 tggttggctt gtcgattgtc ccaggttgtt ttggctggct gtgggcagcc aaacggtggg
    15421 gagcattacc ttgcctgacc gcgaatttgc tggtgcaggc gatctgcgtg ctgttaactc
    15481 tcgccagcag ctctccttta ttactcatca tcagcagtat tggttttggc ggcaccttta
    15541 tggggacgac ctcgctggtg atgaccatcg cccgccagct tagcgtgccg ggaaatctta
    15601 accttttggg ctttgtgaca ctcatttatg gtatcgggca aattcttggc ccagcgctga
    15661 ccagcatgct cggcaacgga acgtcggccc tcgccagtgc cacgctctgc ggcgcggcgg
    15721 cgctatttat cgcagcatta atctgcggga tgcaaatatt caaattgcat acgaattatt
    15781 cttaagtaat ttaacgaatt taacttttca gtacatcacg taaaataatt aaaacatcct
    15841 ttttctttgg aggtactcag ccaccgccgt tattgttcct ggtttagcaa ttccacggcc
    15901 aattcattat ttctggtcac attatagtca ggatcgtttt atatctccgt gctagaaaaa
    15961 acaagtttaa ttctgttttt tcacggaagg tcactatgtc ttcaacaaat gcgcatcaaa
    16021 taaaaaataa tgatcaaaac tctttatgtg gtctgggtga caaaatacgc cgcctgaccg
    16081 ccggagtttg tttatttacc caaatttttt tccccgtcat ggcgacggca caaaatgtgg
    16141 tacacgctaa acctcagacg acagtttcat ctccgtcccc tctaatagaa aataatactg
    16201 tgccttacac gcttggtgcg ctggaatcag cgcaaagcgt tgctgatcga ttcggtattt
    16261 cactggagga gcttcgtcgt cttaatcagt tccgtacttt tgctcgcggc tttgataacg
    16321 tgcgccaggg tgaagagctg gatgttccgg caacaacctt gcagaaaagt catgagcaac
    16381 aaaatgccgt accgcctgcg aatggcgaaa acacgctgga gaatcaaata gccagcactt
    16441 cgcagcgagt tggcaccctg ctttcacaag atatgaatag tgagcaggcc agcggcatgg
    16501 cgcgtggttg ggcgtcttca gaagcctcag gcgcgatgac tgattggtta aataactttg
    16561 gcactgcgag aatctctctg ggtgtggatg aagatttcag cctgaaaaat tcgcaattcg
    16621 acttcctgca tccgtggtat gacacacctg attatctgct cttcagccag catacccttc
    16681 accgaacaga cgatcgtacc cagatcaaca ccggtttggg ctggcgtcat ttcaccccca
    16741 gctggatgtc aggcatcaac ctgttttttg accacgacct gagccgctat cactcccgcg
    16801 cgggtcttgg cgcagaatac tggcgtgatt atctgaagtt gagcagcaac gcttatatcg
    16861 gcctgactgg ctggcgtagc gcaccggaat tggataatga ctacgaagcc cgcccggcca
    16921 acggctggga tttacgcgcg gaaggctggt taccagcctg gccacaactg ggtggaaaac
    16981 tggtctatga acaatactat ggtgatgaag tggcgctgtt tgacaagaat gatcgccaaa
    17041 gtaatcccca tgctattacc gcgggcctca actatacccc cttcccgctt ctgactctca
    17101 gtgcggaaca gcgtcagggg aaacaaggtg aaaatgacac acgttttgcc gttgatctga
    17161 cctggcaacc cagcagttca atgcagaaac agcttaaccc ggacgaagtg gccggacggc
    17221 gcagtctggc cggtagtcgt tatgacctga ttgatcgcaa caacaacatc gttctggaat
    17281 atcgcaagaa agagctgatt cgcctgagtc tactggatcc ggtgaaagga aagtctggag
    17341 aaataaaacc gctggtttcc tcgatacaga ccaaatatgc cctgaaaggc tataacatcg
    17401 aagccgctgc gctggaagct gccggaggta aagtcagcac gtctggaaaa gatatcacgg
    17461 tcacgctgcc aggttaccgc ttcactaaca ctccagaaac cgataataca tggtcgatag
    17521 acgttaccgc cgaggatgta aaaggtaacc tgtcacgtca tgaacaaagc atggtcgtca
    17581 ttcaggctcc gacattaagc cagaaagatt ctctgttatc cgttaatccg ctaaccgtgg
    17641 ctgcagataa aaaatcgacg accacattga ccgttactgc gcacgattcc gacggaactc
    17701 cggtgccggg gctggcgctg caaacccgca gtgaaggcgt tcaggatatc accctgtctg
    17761 actggacaga taacggtgat ggtagttaca cacagatgct gaccgccgga acgacatcag
    17821 gttcagtaac actgacgccg caaattaacg gtgagagtgc ggtaaaagaa tccattgtcg
    17881 ttaatatcgt ccctgttgtc tcatcccgcg accattcatc aataacaatt gataacgtat
    17941 cgtattatgc cggagacgac atcaaggtta gggtggaact gaaagacgac agcaatcaac
    18001 cggttgcata tcaaaaagag gaattggtaa aagccgttac tgtcgaaaac agcaaacctg
    18061 gcgccacgat tgtctggcac gaagagcagc ctggcgttta taccgcgaat tatccggccc
    18121 ataagcaagg gacagcacta agggcacaac ttagccttca caactggaat gctccactgc
    18181 aatcacatat ttataacatt gaggcaaacc agaataaggc tcgcgttgcc acattatcag
    18241 cgacaaataa tgacgtttac gccgataaaa agacatttaa taccctcacg atcaacgtca
    18301 ctgatgagag tgataatccc ctgacaaatc atcaggttac ctttaagaat gaaaaaggaa
    18361 gcgcggagtt tgtcgaaccg ccgcagcaaa atacggatgc atatggtgtt gccacaataa
    18421 acatggtaag tcaggttgcg gaagaaaata cgattagcgc cacgctgcca aatggtttct
    18481 cacaacggat aattgcgaaa ttcgttagcg attcgagtac gccaaaattc aaacaactgg
    18541 ttgccgatcc agataccatt attgctggca acagccaggg cagtactctg accgccaccg
    18601 tcacagactt tcataacaac ccgttaaaag atatgaaagt gaattttgtg gcacctggtg
    18661 gctcgcaact ggacaacacg tccgccacaa cagaccagtc cggtattgtg cgagtgcacc
    18721 tgaccagttc aaaagctggt agctattccg tcgatgcctc gcttgaggcg gataaaaata
    18781 ttcaccagtc ggtcacgatc accgtggtcc caaacaggga acaatcggta atgaccttga
    18841 atgccgggtc gggcagtgcg atcgctaaca atacaaatac cgttatcctg acagccagtg
    18901 tgaaagatgt ttacggacac ccgttgccgg atgaggatgt gaaatttacc ttgccagcct
    18961 ctatgaccgg gaacttcacg ctaagtagtg aaaccgcccg caccgatgca aacggcgatg
    19021 ccgtggtcac attgcgtggc acaaaagcgg gtgagtttac agttacggcg acgctgacca
    19081 gaaataatac cgttgctcat cagcaagtca cttttattgg ggatacaaac agtgcgcagc
    19141 tccagccgct gactgcctca ttaaatacca ttgttgcggg taacagtacg gggagtaccc
    19201 tgactgcaac gatcctggac gcttaccaaa atccgcttaa agaccagctg gtcactttcc
    19261 agagtaacga tgtcactcta agtggaacag aagtcaccac caatacactg ggtcaggcga
    19321 cggtaacaat gaccagcaat attgccgggc aacataacgt cgtggtgagc cggaaagcgc
    19381 aagcctccga taataaaacg tttagtttat cagtgctgcc ggatgaaagt tcggcgaagg
    19441 taataagtat aaccggagcc gaaaaaacga taacggttgg cgaaaacatc acgctacgga
    19501 tactcgtcca ggacgcgttt aacaatgtaa tagcgggtca acgcgtcaga ttaagtgcgc
    19561 agccaacagc tcacattacg ataggcgata cggcttacac cgataataac ggttacgcgt
    19621 acgttaacct tctcagcacc cagcctgggg tttatcaggt gacggcaacg ctggacaata
    19681 acagtagtag taaggttgat gtgaatgtgg caaatggcaa actcgagtta acatcatcga
    19741 agccagaaac cacggtccat aacagtgaag gtattacgct gaccgcaacg gcgagaaatg
    19801 ctcaggatga attaatgcct ggacaaatta tcacctttag cgtaacgcct gaaggtgcaa
    19861 cgctaagcaa tacaggggaa atacttactg accagtatgg tcaggccaaa gtgacgctga
    19921 ccagtgacaa agtgaatgtc tataccgtta cggccacaat gggcaaagat gttcccgttc
    19981 agagccaggt aacggttgcg gttaaggcag atgctaaaac ggcacatgtg gtgagcgtcg
    20041 tggcttctcc tgacaccata accgccgacg gcatcgatag cagcaccgtc acatcacgag
    20101 tagaagatga ttacggattc ccggttgaag gtgtcgatgt tcgttacacc ttagacacca
    20161 aaggtcgccc ggtagttaat attccaacta cgcgtaccga tcagtccggg caagtcacgg
    20221 cgacaataac cagtacattg gcagaaacct taacggtcaa tgtgcaagtt cctggtacag
    20281 ccaaccaatc cgcagctatt accttgcttg ccagcacggc ggatgaaagt aagtcattat
    20341 tgaaatccga cgttggcact ctgatggctg actaccagca tagcgcgaaa cttacgctaa
    20401 cattgcaaga caagtacggt aacccgatag tgacgtctga tcatctggaa tttgtccagt
    20461 caggtccctt cgtgaacttt ctcaagttga gcgatattga ttacagccaa agaaattatg
    20521 gcgagtacac cgtgactgtc actggcggaa aagagggaac agcgactctc attcccatgc
    20581 tgaacggggt tcatcaggca aacttaagcg tatcgctgaa tctcatccgc tcgataaaag
    20641 aaatgtccgg tcatgtcact gcaaacaacc ataccttctc cacggctcaa ttcccgagcg
    20701 aaggttttgc aggagcgtat tacacactca acaatgataa ctttgaagcg ggtaaaaccg
    20761 ttgatgatta tatgttttca agttcacaga gttgggtgtc ggtcgatgct tcaggtaagg
    20821 tttctttcgc aaatatcggc gatcaaacgt cagtcacaat aagcgctgtt ccccgacaag
    20881 gaggtacaac ctaccagacc ttaattaagc tgaaaggctg gtgggtgaat aatggaaatc
    20941 ataccaatat ctggctagct gccaatgcgc tctgtcatgc taaaaatgat ggatataatc
    21001 ttcctggcat cacacatttg acgtctggcg aaaacaaacg cacgcaggga tcactgtatg
    21061 gtgaatgggg gaacgttgga gcgttttcca gtaattcgca atttacacaa ggagcttact
    21121 ggacaagtga atctgatgat tacaatcggc actactatgt gcagatgcta acgggtatga
    21181 ctggaagcga cgctgattcc agcccccaac tgaccgcctg ccgtaaatca ctttaatttt
    21241 agtaggttat taaggatacc cttgtgcgag ggtatccttt ccgactgata acaaacgcta
    21301 cattacctga tgcgctgcgc ttatcaggcc tatataaccc ctgcaatata ttgaatttat
    21361 tgtttttgta ccccagccgg catgaacggc cactcaagaa actcaacagc aaccgttgat
    21421 gtcagccaga ccccgttttc cgcttgccag aaagggaggc ctcgtttcgc catttcctgt
    21481 gctttgacgg ttaaaataac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct
    21541 tcatcagcag agagatgtac gtagtgacgc tcacctgcaa tcagcccctg tttttttatc
    21601 tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt tttttcggca
    21661 aaggaaatgg cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca cctaccatca
    21721 ctggaataac taaaacgctt tttatcgctg gtggcaacca cagtatccag caatgcacgg
    21781 gttaaccgct tgcccgcttt ttgggcacaa agtatgagct tatcgatatc cgcccatcct
    21841 tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac
    21901 ttgctcgtat ccgccagctc tttttcgttg tactttgcca tctcgttttc gtccctggat
    21961 tttctgtttt tatttactta tgtcattgtc actgggttta tgaatttact gcaactcaat
    22021 cctgcgcaga gccttttcca cacagcgtaa tttttccgct atgcaaaaaa cgcatagcca
    22081 gaaacatact tgcattttcc ccatacccac atggctgata aggtttttcc gctccaggaa
    22141 ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac
    22201 aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa gggtatctgg
    22261 ctttttttct caccataatc tttttttccg gtgttttttc cgggacagac agttggtggc
    22321 gcgtttttga tttttccgtg ctgaacgggt cgtttgggca actgcctggt gcgaacggag
    22381 ccaccacctc gtttcgcggc gcgggtggcg cgggggctaa agatggcttt ctctttgcgc
    22441 tggagctggc accgtcggtc attctctctc tggggattat ttcaattacc gatggtcttg
    22501 gcggattacg cgccgcgcag cagttaatga cgccagtgct aaaaccgttg ttgggcattc
    22561 cagggatctg ctcgctggcg ctgatcgcta acctgcaaaa caccgatgcc gccgccggta
    22621 tgaccaaaga gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg
    22681 catatcaaac cagcggtagc gccattatca ccaactactt ctcttccggc gtcgcggtgt
    22741 ttgcctttct gggcacgtcc gtaatcgtac ctttagcagt catcctggtg tttaaatttg
    22801 tcggagccaa cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag
    22861 gagcgcaagc atgacaactc aggtccgcaa aaatgtcatg gatatgttta tcgatggcgc
    22921 acgccgtggc tttactatcg ccaccaccaa cctgctgcca aacgtggtga tggcgtttgt
    22981 catcattcag gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc
    23041 ggtaatggcg ctgtggggat tgccaggtga agcggcaacc gtccttttag ccgcattaat
    23101 gagtatgggt ggagcagtcg gtgttgccgc cagtctggca accgctggcg cattaaccgg
    23161 tcacgatgtc accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt
    23221 cggtcgctgc ctggggacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt
    23281 ctgcgtgatt aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt
    23341 taccatgatt gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc
    23401 gccggaagat cggggagttt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc
    23461 cagtaatatc ccttctgaca ttgtaccgga ctgcacggtt gtcgatctca gcgggcagat
    23521 cctctgccca ggttttattg atcaacacgt tcatttgatt ggcggtggcg gtgaagcagg
    23581 tcccacgacg cgcacgccgg aagtggcgtt aagtcgtctg acggaagcgg gcgtcacgtc
    23641 agtggttggt ctgctcggca ctgactctat ctctcgtcac ccggaatccc tgctcgccaa
    23701 gacccgtgcg ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt
    23761 cccttcccgc actattacgg gttccgtgga aaaagacgtg gcaattatcg atcgcgtgat
    23821 tggtgtgaaa tgcgccatct ctgatcaccg ttctgccgca cctgacgttt atcacctggc
    23881 taatatggcg gcggaatccc gcgttggcgg tttgctcggc ggcaaacctg gcgtcaccgt
    23941 gttccacatg ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc tggaaaactg
    24001 cgatgtgccg atcagcaagc tgctgccgac ccacgttaac cgcaacgttc cgttgtttga
    24061 gcaggcgctg gaatttgcgc acaaaggcgg cactatcgac atcaccagca gcattgacga
    24121 accggtcgcc cctgccgaag gtattgcccg cgccgttcag gccggtattc cgctggcacg
    24181 cgtcaccctc agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt
    24241 aacccatatc ggtgttgcag gttttgaaac gttgctggaa accgtgcagg tgctggtcaa
    24301 agactatgat ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt
    24361 ccttaacctg agcgggaaag gtgaaattct gccaggcaat gacgcagact tactggtcat
    24421 gacgccagaa ttgcgcattg agcaggtata cgctcgcggc aaactgatgg tcaaagacgg
    24481 caaagcctgc gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagtc gcccgaatgg
    24541 acgtagaatg cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgtggtgcgg
    24601 ttttgcataa tattgaaaca aaatggcttt atgattttct gaccctggaa aaatgccgca
    24661 acttttccca ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc
    24721 gcgcgctgga acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac
    24781 tctcggaaca agggaaaatc tttcattcgc agatccgcca tctgttgcaa cagttagaaa
    24841 gcaacctggc agagctgcgt ggcggcagcg attacgcgca acgtaaaatc aagattgccg
    24901 ctgcacactc tctttccctc ggtctgttac cgtccattat cagccagatg ccgccgctct
    24961 ttacctgggc gattgaagcc attgatgtcg atgaagcggt cgataaactg cgtgaagggc
    25021 aaagtgactg tattttttcc tttcacgacg aagatttgct ggaagcaccg tttgaccaca
    25081 ttcgcttatt tgaatctcaa ttgttccccg tctgcgccag tgacgaacac ggagaagcac
    25141 tgtttaacct cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg
    25201 ggcgattgat taatcgcacc ctgacgcgcc acagtaagtt gagtttcagc accttttttg
    25261 tctcttcgat gagcgagctt ttaaagcagg ttgccctcga cggctgtggg attgcctggc
    25321 tgccggagta cgccatccaa caagaaattc gcagcggaca gctcgttgta ctgaatcggg
    25381 aagaactggt gatcccgatt caggcctatg cataccgaat gaatacccgc atgaatcccg
    25441 ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgtgcttagc tgacattctc
    25501 cagcgtcgca ctacgccaac cgccattatc ccagaccaga tcaaacgtta gcggttcatc
    25561 gtcgtggtac agcattatct caaggctgat cgccagcgtg tggcagtacg cttctttcaa
    25621 attgacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac gcagctgacg
    25681 gcaataccag ctctgaagat gtttgtcgct ctggttatgc acttgccact cgggcatgcc
    25741 tgaaacaagt aatgacggta atgccgaatg aggagaatta aaaagtgtga ggagatcgct
    25801 gcataccgat ttacgcagca atgacgtttt attgcctgaa atttcaggta aaacagcctg
    25861 aagtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc ggcttgccct
    25921 gaatgccggg caccactgta ttgatgctca ttttccatag ccattccctt ttgttcatta
    25981 catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa ccttacttgc
    26041 ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact
    26101 caaaaaaaaa ccgctagtat attctgaata ttccgaatag ttacgtagat gaaaatactt
    26161 gtcaccgccg ctacttaatt ccggtgggaa attatttatt tccgcataat cagccatgta
    26221 ttatttgaaa caattagttt gtaaataata taaattgata acatatcaaa tacatcaaaa
    26281 ataccattca caggcattat taatatagac ctcaatatag caacatcaaa ttccaattag
    26341 taataagata tgcttttaaa ttgcatgcaa ataattttaa atgagcgttt tttgatactt
    26401 tagagtgttt cccacccttt aatatcactg tgtagagttg atatcactac aacagaaaca
    26461 tgcatgtcat ttttttactt ctcttttact acagaaaaat ttatctattt ttctaaacat
    26521 cagtgaaata ctcataaaag gtaactatta tgtctatgcc aataagcaat gcccttcaga
    26581 gtcaaattat tacagatgat catttccttc atcacccaaa agtcgacaat gagttaacac
    26641 gtcgatatga atatgcacgc atggatacgg aaaatatcta tcttctgcct cttgctcgtg
    26701 gcaacaacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt aatattggca
    26761 aacagtcctg gccatttaat tatgtaacag aaacatgcag cgaattcaat ggcattacaa
    26821 caacagggcg tatgctctat cgaaacttga aaatcacctc agctttagat gaaatatatg
    26881 gtggcatatg taaaaaggcg catgctgcca cagagttagc agaaggttta cggctaaatt
    26941 tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa ataacattag
    27001 gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt tcaacccttc
    27061 cggcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc tatattgatc
    27121 agataatgac ggtaaccggg gtagcaaatt cttccggatt cgcccttgcc gcattactta
    27181 atgctaatat tggaatggga aatgacccaa taattggtat agaagcctat ccaggtaccg
    27241 cggagatcca tgcaaaaatg ggatataaag ttatctccgg tgatgaagat gcgccactca
    27301 aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa aatggtgaat
    27361 ggaattatat tggtaaatga ctactgagaa gaatgagtac taacattcag gttatacaga
    27421 tatttaaaca ataccctgca atttacaaag taatactcat tgcgacttct taatttaaga
    27481 aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg actttatcca
    27541 gcggccatga atcaaacaga tagccgtcaa aataaatatc gtcaacgttc gagaattcca
    27601 gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc gcacgggcat
    27661 cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct ttgcgataca
    27721 ggctgtcatc gagatggctg tgcaattgga tccacattgg attgttttcc cgccactgcc
    27781 aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcggca atggcgagat
    27841 gaaactgcat gtcaccactt tcgctgctgc ccggcgcgct ggaagcaagc tcacgctctt
    27901 ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc agggcggcaa
    27961 attccgcgat attactttcc agtaactgac gcgcctgtaa cagctcaaaa ggaccggcat
    28021 cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta tcaagaacat
    28081 aaataccggc accccggcgc acttccacca gccctttgat ctccagcatg atcagcgctt
    28141 cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggcagcc
    28201 gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc ccgacttcct
    28261 ggtaaggtct ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta
    28321 atcaaatttt tgccggatgc ggcgtgaacg tcttatccgg cctacaacgg gcaattttac
    28381 ggcacccgtt aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggca
    28441 aaaaaacgaa aactggtcaa ccaaatatgc gtttgattga ccagtaagtc tgtagggtaa
    28501 tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag ctgctgatat
    28561 gccgtcgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc aaaaataccg
    28621 ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt aatcttctgg
    28681 aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc cacgccctgg
    28741 gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc gccgttttgc
    28801 aggtgcagac gcaccggatc caacagacgc tgcggtaact tctggctgcc gtccatcgca
    28861 atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag cagcgtcgca
    28921 taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc
    28981 agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat agtttcatag
    29041 ccgccgaggt aaccgaggta tgccaggaaa gagtggctgc cgttcagcat acgcagtttc
    29101 atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc ccagtccgga
    29161 cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca ggcaatggcg
    29221 cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc
    29281 ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat ccatgccgcc
    29341 agctgcggat cacgtgcctg agccagcccc agaaccgcga ccttcgccac atgaccgttt
    29401 tcacgcacgt tgtcgcagga catcaccgta aacgctttca gccctttttc acgacgcaga
    29461 cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt ttccagatcg
    29521 tgcttgatca gcgggttatt gagatccaac tgaccgcttg ccgcatcggc acagtagcct
    29581 ttttccgtca ccgtcaggga gacaatcgcc gtctgaggac gcgccatcgc gttgagaata
    29641 ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat aattttcagc
    29701 tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt tttcaggttt
    29761 tcgatcagca cgcggtcgtt gcctggcatc aggttaactt cgcagatgcc ccagtcgctg
    29821 tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg aaacgcaccg
    29881 caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg gcgggcaacc
    29941 ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcagagagtg
    30001 acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtggatat taacggctaa
    30061 agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct ttcagacgac
    30121 caattgcgga gtaacctggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt
    30181 ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt
    30241 caacaatcgc tttcaccact tcatacatat caacgtcacc gttcaggtgc gccgcttcgt
    30301 ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatgcgcg
    30361 gaccaaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg taggaaccgg
    30421 tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac tgcatatctt
    30481 caatggtaga aacaatgcgc ggcaggccga ggattgggcg cggcggatcg tccgggtgaa
    30541 cagccatacg gacgccaact tcttcggcaa ccggaataat ggctttcagg aagacagcaa
    30601 agttttcgcg cagtttggct ttatcgatat ctttgtacag ctccaggtgc ttacggaact
    30661 ggtcgagggt atacccttct tccgcacctg gcagaccagc aatgatgtta cgggtcagac
    30721 gcgctttgtc ttcgtcgctc atagtggcga agcgaacagc tgcctgagca atttcttctt
    30781 cggtgtaatc cgcttccgcg cctggacgct tcaggatatg catttcaaat gcagcgaatt
    30841 cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg aggtcagtac
    30901 gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgccg cactgcgcca
    30961 ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca gtgtgggttt
    31021 tgatatcttc gtgaattggc acgctttcta cgacagacca caccaggcct gcgtcttcaa
    31081 cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccattc gggatatggt
    31141 gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccggat
    31201 cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt
    31261 tccacagcaa agagacgcgg aaccctggag ttgtttaggt atccggatga acaaccagac
    31321 cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaagacat
    31381 gtcaacctga attctaaaat tggttaacca catcacaaga atttcactct cctggttgtt
    31441 gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg acattcatcg
    31501 caacaatggt tgaccaattt acataacatg tcgcccaaaa taacaacggt tcaaccaggt
    31561 aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca ttggtctgat
    31621 gctggtactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg tggcagcgct
    31681 gtccgtcggg atgctggcgg gcatggattt gatgtcgctg ctgcacacca tgaaagcggg
    31741 cttcggcaac acgctggggg aactggctat catcgtggtg ttcggtgcgg tcatcggtaa
    31801 attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg cgcgtctcgg
    31861 tctgcgctat gtacagctgt cggtgattat catcggcctt attttcggtc tggcgatgtt
    31921 ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg ccgaagctaa
    31981 aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg cacattcact
    32041 gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcag atatggggat
    32101 ggtttatatc tatggcgtac tggtgacgat cccaagtgtc atctgcgcag gtctgatcct
    32161 gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag cagatcaacc
    32221 ggttgatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc tgatcccagc
    32281 gatcattatg atctccacca ccatcgccaa tatctggctg gtaaaagata cccctgtctg
    32341 ggaagtggtt aactttatcg gttcctcgcc gattgcaatg tttattgcga tggtggttgc
    32401 attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga acgcttttga
    32461 aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg gcgtgctgaa
    32521 gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt cccacggcaa
    32581 tatctcgccc tacatcatgg catggctgat caccgtactg attcgtctgg cgacgggtca
    32641 gggtgtcgtt tcggcgatga ccgctgccgg gattatcagt gcagcaatcc ttgatccagc
    32701 aactggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg ctgcgggttc
    32761 caacaccctc acccacatta acgatgcatc tttctggttg ttcaaaggtt actttgacct
    32821 gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact ccgtggttgg
    32881 gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag cctgccattg
    32941 gcaggctctg ttcggattgt cggtaaagtg cgctttgttt atgccagatg cgacgctgac
    33001 gcgtcttatc tggcctacaa agggctaacg tgcaggtttt agcttcaggt aatattgcgt
    33061 acctgcatta gcaatgcgct gtaatttttt tattgataaa caaaagtcac gccaataatc
    33121 gattgcacat tccctgcagt cacctgccct ccggtacgtg cgtaatttgc cgttaatccc
    33181 agacttaccg ccgaagtccc tactgctcct aacgataccg tgttattcgc tggaataatc
    33241 gtaccgttgc gcgtcaactg tacgccgacg ccctgcgctg gtgaaaacga cgcggtattg
    33301 gtgaaaatcg agttgcccgc atctgcggtt gtgccggaga ggtaaaaccc caggttttgg
    33361 cttttcgcac aataaacggt aagaggaatc ggcaccgaac cgggatagtc cggcagagta
    33421 acggtgacat cacgagcaga aacatcacag ccgccagtag gcaccaccac atcattattg
    33481 gcgtaaatat tccacacaaa ctggaaatca tcgctgttat agttgttggt ctgtcgcaaa
    33541 attagcacgg caattaatga gccagcttta atcgccaccc cgccagcact gctcacaggc
    33601 gtcaaataaa gcgccaccgg ccacggctta tccgttctcg aattataaac aacccgcggc
    33661 gtttcgctgg tggtaggaaa tggatagcta ctgccactat attttacggt cccggaaaaa
    33721 ttagataaca cgccgccata agccgagcct cgttgcagtg tgacatagtc tgtaatggtt
    33781 tccgggtaat cgttatggca aaagatttgc gtcgaaagat ctacgaccag gttttgcccc
    33841 acattcacgg cgggcgcaag gtttacataa acattggcgc tgccaccgcc aatagggata
    33901 gcggtaccat tggcggtttt acaggcgaat gaccaggcat ttaccgacca gcccatcagc
    33961 agtacagcaa acagggtaat aactcgtttc attacaatca tctcttcggg ttcagctgta
    34021 ggtataggtg atgctaatca ctgcctgaat ggttccctga gtggctccgc catttaccgt
    34081 caatgctctg acctgtaacg ggaagtgcgc tgattgtgag gaatcatcca cctgaactgt
    34141 tttggttgcg ccactattca atgtgttgcc actgtcatcc tgtagctcta actggatgtt
    34201 ttgcgcggtc ccctggtttt tataatatcc ggtactgtcg gctgccccgc tgaagctggc
    34261 ggtgaccctc gacgttccca ccggacaatt agtcaactca agcgcaacat catgccaggc
    34321 cgatgccgcc ccggcagaca tcagactgaa agaataaaga tcgccgagat ctaccgtggc
    34381 attggtggtg gaaaccgtgc acggtttggc gacgacctta ccgttcaccg tgatggtgac
    34441 atcggctgcc tgtatcgtcg cacttgcgag cgccaacatt gccgccaata aatacccacg
    34501 tttgcgccat ttcatgagca tctccagtta ctgatattca agagtgaagg tagccgtggc
    34561 attgatatgc cccgcagtga caggcacctg tgtcgccatt agccgggcgt aaaaattcag
    34621 cgtatttggt ttacccggcg tcagggtcgt ccacgaaagc gcggacgatg gggcattaag
    34681 gggtatctga ttttgttgct cattcagaag ctgtattccc agtcccgcag ccgctgacac
    34741 cgtattttca agtgcaagca ggttggcatt gtggctatct gcaacaccgg taaacccaac
    34801 ttttacggca gaaacggcat taccacaggg tgacagcaaa atacgaaatg gaacgacagg
    34861 agtcgtcgcg ccaatgttgt taaattgctt cgccgcgttt tccatcagat caacagtaaa
    34921 attggttgat tcagcggcca cactacagcc gttatctctg acatagccgc ggatagtaat
    34981 cgtgctatcc gcagccaaag cgtgacttac cgacaaccac aaaaaagcgc acagaagata
    35041 aaaaggtttg tttctcatca cgccccctta acgacattca gctgatagct gggttaataa
    35101 ctgctgctga ctctctggtg gcagttgata attggcgaca cagtgagcat tttcctcttc
    35161 tccccatttc acctgaactt ttcccgctaa aggcattccg ctgaggtaaa cctgaccatt
    35221 atccgcaaca atgccgctac tctggctact ctctgatgtc accatcgccc caaacggcag
    35281 cggcttatta ttgtgggtta gcgtcatgag cagttttatc ccaacgcgcg ctttaaactc
    35341 tgctcgcact atcgccccac gagtgggaac aacgttagcg accgcgttat ctaaatcgac
    35401 gttatcagcc agggtattgg tatccagcgc tactctgttt tcccgatatt cagtggcata
    35461 aggcagcacg gcataaccgc gccagtcggt acgcaccccc gtctggtttt cgacttttgc
    35521 atcttttgcg ccaggcgctt taacaagcac caccgtatcg tttaacggct gccccagcgt
    35581 tacgccattg gcatgagcca gtaccccacc gctgactcca taatagagct gcttaatatc
    35641 atcgctatgg ctgtaaccga tgttggcatt gccgtaacca ccgcgataat tcagcgtggc
    35701 gtagcctgtg cttccgctat taccatcgcc tcccccggca tagccggttt gcacgctata
    35761 gctgaggttg ttgtcttcca gcaacgtacc gtatacaccg gccagattgg tcatccgacc
    35821 gttgagatcg tgtgacatgc tgtagctggc actggcatgt cgccactgag atttactgtc
    35881 agaacgcagc cagtggctga aaggaatatt gacgttaagc gctaacatct gatctcgtcc
    35941 tttttgccag gcgtttttcg tcaggctata gctgagcgtc cagttgatat cttcgaatgc
    36001 agtatttaaa ccagcctgga attgctcatc gacattactc gttccccaat aagtttgatg
    36061 gctaccactc aaatacagtg ttgatgtgcg cccgagttgc tgagtaacgg tgagctgtaa
    36121 tttcccgcgt ttgttataag cgaggttgta atagtcggtg aatttcggct taacctgaat
    36181 aactccatcc tgtgtttcga tgttgtagcc attcattcga ctgtatgttg tatcagcgaa
    36241 attaaaatat ccgctggtcg aataacggta acccactaac tgaatattcg tgcctgactc
    36301 attgagcgat ttgttataga gaaaacgcac cgattgtccg tcatgctgac tgtcatcggg
    36361 aagtgtggaa ttagcctgcg tcatatccac agacagagcg cccagtgccc ccatgttttt
    36421 cccgataccg aaattaaaag cacgataacg atccgccagt tgcgttccac catatattgt
    36481 ccagccagcc ggaaggccgt ggagtaatgt actctggaaa aagcgaggtt tctcctgctg
    36541 cgcatttcca ctacggtatt ctcctgccgt aatggaataa cgtgtatgcc cttcacgttg
    36601 caaaagcggg actgacgaat agggtacggt aaaaatctgc gtgctaccgt cagcctcttt
    36661 aatcgttacc tgcaagtcac cactattacc tgcggcatag atatcgttga tggtaaaagg
    36721 ccccggcggc accgtactat tataaatgtc atacccattt tgtttaatag tgacctgtgc
    36781 agtaccacgc gcaataccgt ggatcaccgg ggcaaatcct ctttggctat cgggtaacat
    36841 attgtcatct gaggccaatt gtgcgccgcg aaagttaata ccatcgaaaa tatcgccctg
    36901 agtataacca tcacccagcg tcagccggga acgtaacgga attatgtctc gctcaagcca
    36961 ggtattgata tgctgccatt tatttttgct acctgatgaa ctgtcgctac tgttataact
    37021 ccaggtggta ttgtcgcgta aacgccacgc accaatattt agcccactct gtaggtttaa
    37081 atatgcataa tggctgttac taccaatccg attctgtaca ctatttccgc tgaaattata
    37141 attgagcaag ccggcattaa taccgggatc ccacaactca ggaggaatat atccacgcgc
    37201 gcgattactc ataaatgcct gagggatcgt caggttgagt cgctgctgac caacatccag
    37261 atgcgcagta gcgtcatgga tcattgaggt taatggcacg caggcatcat ccgccagcag
    37321 attcataccg gagacagaag ccgtattcag ccccatactg gcaagttgcg cgcgtgtcag
    37381 gcagggaaca atcccttgtt cactgtcgcc cgtgttaaat gtgacatcac gcgttgccat
    37441 ataaccatta ttcaaataga tatcgacgcg atacgtccct ggcggtaatt cttgcccatt
    37501 ttcaaaacgc gataaatcgg ccacagcctg gggatcatcc gctaaaaagc gcggattaaa
    37561 atagagttcg gcagatgaca aaggtgcctg tgcggcaaaa gcacaggcga caaagagccg
    37621 gacaaaaaaa ccagccaaac gatgcttacg aatatgcaag cattgtgtgt ttcgctggta
    37681 aagtcttaaa ttcagatatg acattattcc tccggtttaa aatcacccgg cagtcaattc
    37741 tttttattca ggcgaaaaat tccccctgcg ttattccatt acgcccgtca ttttgggggt
    37801 aagtgcgcca taatcattta ttgttcggta agtaatattg cttcctgcat cagaaggcaa
    37861 tttaaccgtg ctttcgccca ttggaggcac caatgcattt tcaagaaccc gggttccggc
    37921 attcaactct gttaccgtca ggtaataggg tgtcgggtta atcagcgtca gagaattcgc
    37981 gctacgacga aatcttaatt tttctgcggc ctgatcgggt ggcaacgcta atttagccgg
    38041 gcgatagtac agtttaatgc ggctgataat tgcgagctgt agcgtattct cagtcaattt
    38101 tgatttatcc attgacggaa tcgctttaac gttcatccag aataaacttt cccgatcctg
    38161 tggcaattgg ttatttgttg catcaagaat acgtaaggta ttctcttttt ttcccttcat
    38221 cgcaaacaga ggaggcgtca cgataaaacg accatccttt acaccatcgg cattttccac
    38281 ccatgattga attaaatagg tgctattttc atcattattt gtcacggcaa gttgcacttg
    38341 tttttgcccc gccggataaa ttacgcgggt cgcacctaag gccactcccg cttcagcgcg
    38401 tccggcaacc atcattgcca tgaacatcag gatacctgcc agcaagcaga atgttatttc
    38461 ctgcgatttc cttacattga cgtttttatt actcactgtc ctgttcctgt tatcacatta
    38521 tctgctgaac aattactgat aggttaaaga gaaccaggcc tgggcattgg cgatgccgcc
    38581 agtaacccga cgcccggtag cacgatattt ggcgatgaaa tgtagcgaag tagagcctga
    38641 gtaaagccgt ttccagtttg ctggaggacg attaatcggt acgaggtttc cttcatcatc
    38701 aaataacgct acgccaatat ttgtggctat ccctggcccc tctcccacgg aaagcacatc
    38761 cggattttta ccatccgcga caccgtgaaa cgccacaccc acacgttcac tcaccaccgt
    38821 gctacattcc cgtaaatgaa taacaaaagg caccggtgcg ctatcttccc ctaccgcatg
    38881 aaaccggtta ctgctgattt gccccatatt gaccgtcatt tgtttatcac cagcttcaac
    38941 tcggcaagtt tccgcaataa tgacgccctg aaattgcata tttccgccgg gcagcgtggt
    39001 attccattta tttccggcca gagcaaacat tggcagtaac gacgctaata gaaataatct
    39061 cttccttttc atcgttattt ttatcgcaca agggtgggca tccctgcccg taatgacgtc
    39121 cctgaacctg gtaggttatt gatactgaac cttgaaggtc gcatccgcat tagctgcgcc
    39181 aggggttgcg gcaccggttg caaaataacg cgcctggaac ggaatggtgt tggttccgtt
    39241 attcagggtt gtttctgaac taaatgtcgc accgtccagc gccagcgcag cacccgttct
    39301 gtccaggatc tgcacaccaa cgtttgttgc gctacccgca gctgaactct gcagagccag
    39361 tacatcagta tgcgtagcat caattgccgt acctaagaag gcaacagcgg cttttgtggc
    39421 aacagtggta tcgcaatcat tcagctgaat gttaaaacca acggcagagc tggttgctcc
    39481 agcctgcttc aggctagcgg tacgaacctg gcccaactga acggtttgat caacagagcc
    39541 tgcatcaacc gcgcaagcgg cgttaacaac ttccccttta aagtgaacgg tcccaccatt
    39601 taccgtcgta gtatcggcca gagccgctgc ggaactgagg gacagagccg acagaacaac
    39661 gattgccaga gttttaattt tcatgctgct ttcctttaaa aaaactattt ctaaatcgac
    39721 atgggcagtc gttctgtaca ctttgttttg tcaacgagtt tgactgccaa cactgcacag
    39781 ttttccccca aaagatgaaa catttggggc cattttgact catagaggaa agcatcgcgc
    39841 acaaactttt tcagtttatt tgttggctta atattctatt gttatcttta tttatagatt
    39901 tttatattgc atgaggtggt ttttggagag aagaatg
//
LOCUS       DAOTRK010000021        39813 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.021, whole genome shotgun sequence.
ACCESSION   DAOTRK010000021 DAOTRK010000000
VERSION     DAOTRK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.021"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            307..1032
                     /locus_tag="QIE41_001153"
     CDS             307..1032
                     /locus_tag="QIE41_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDS3530065.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            990..1667
                     /gene="bglJ"
                     /locus_tag="QIE41_001154"
     CDS             990..1667
                     /gene="bglJ"
                     /locus_tag="QIE41_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDS3530066.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1705..2493)
                     /gene="fhuF"
                     /locus_tag="QIE41_001155"
     CDS             complement(1705..2493)
                     /gene="fhuF"
                     /locus_tag="QIE41_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDS3530067.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSTNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2634..2870
                     /locus_tag="QIE41_001156"
     CDS             2634..2870
                     /locus_tag="QIE41_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDS3530068.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLKQG"
     gene            complement(2909..2995)
                     /locus_tag="QIE41_001157"
     tRNA            complement(2909..2995)
                     /locus_tag="QIE41_001157"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2959..2961),aa:Leu,seq:cag)
     gene            complement(3030..3116)
                     /locus_tag="QIE41_001158"
     tRNA            complement(3030..3116)
                     /locus_tag="QIE41_001158"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3080..3082),aa:Leu,seq:cag)
     gene            complement(3145..3231)
                     /locus_tag="QIE41_001159"
     tRNA            complement(3145..3231)
                     /locus_tag="QIE41_001159"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3195..3197),aa:Leu,seq:cag)
     gene            complement(3431..4462)
                     /gene="rsmC"
                     /locus_tag="QIE41_001160"
     CDS             complement(3431..4462)
                     /gene="rsmC"
                     /locus_tag="QIE41_001160"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDS3530069.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPEFDADKFWG
                     EYNVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4565..4978
                     /gene="holD"
                     /locus_tag="QIE41_001161"
     CDS             4565..4978
                     /gene="holD"
                     /locus_tag="QIE41_001161"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDS3530070.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKISMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPMARAALWQQICTYEHDFFPRND"
     gene            4947..5393
                     /gene="rimI"
                     /locus_tag="QIE41_001162"
     CDS             4947..5393
                     /gene="rimI"
                     /locus_tag="QIE41_001162"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDS3530071.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5408..6085
                     /gene="yjjG"
                     /locus_tag="QIE41_001163"
     CDS             5408..6085
                     /gene="yjjG"
                     /locus_tag="QIE41_001163"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDS3530072.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6176..7765
                     /gene="prfC"
                     /locus_tag="QIE41_001164"
     CDS             6176..7765
                     /gene="prfC"
                     /locus_tag="QIE41_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDS3530073.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            8158..8763
                     /gene="osmY"
                     /locus_tag="QIE41_001165"
     CDS             8158..8763
                     /gene="osmY"
                     /locus_tag="QIE41_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDS3530074.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8890..9051
                     /locus_tag="QIE41_001166"
     CDS             8890..9051
                     /locus_tag="QIE41_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDS3530075.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            9173..10246
                     /locus_tag="QIE41_001167"
     CDS             9173..10246
                     /locus_tag="QIE41_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDS3530076.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYCDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            10243..11022
                     /locus_tag="QIE41_001168"
     CDS             10243..11022
                     /locus_tag="QIE41_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDS3530077.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(11056..11919)
                     /locus_tag="QIE41_001169"
     CDS             complement(11056..11919)
                     /locus_tag="QIE41_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDS3530078.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKVLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALKVRG
                     VSRLIFPALYL"
     gene            complement(11891..13441)
                     /locus_tag="QIE41_001170"
     CDS             complement(11891..13441)
                     /locus_tag="QIE41_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDS3530079.1"
                     /translation="MPTSHEIALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLQEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFARTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13699..14478
                     /gene="deoC"
                     /locus_tag="QIE41_001171"
     CDS             13699..14478
                     /gene="deoC"
                     /locus_tag="QIE41_001171"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDS3530080.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKDACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14556..15878
                     /gene="deoA"
                     /locus_tag="QIE41_001172"
     CDS             14556..15878
                     /gene="deoA"
                     /locus_tag="QIE41_001172"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDS3530081.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLTSGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15930..17153
                     /gene="deoB"
                     /locus_tag="QIE41_001173"
     CDS             15930..17153
                     /gene="deoB"
                     /locus_tag="QIE41_001173"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDS3530082.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17210..17929
                     /gene="deoD"
                     /locus_tag="QIE41_001174"
     CDS             17210..17929
                     /gene="deoD"
                     /locus_tag="QIE41_001174"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDS3530083.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18090..18353)
                     /locus_tag="QIE41_001175"
     CDS             complement(18090..18353)
                     /locus_tag="QIE41_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3530084.1"
                     /translation="MIDLENQEREIINLMFSQGISWITAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(18385..19401)
                     /gene="lplA"
                     /locus_tag="QIE41_001176"
     CDS             complement(18385..19401)
                     /gene="lplA"
                     /locus_tag="QIE41_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDS3530085.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPEITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            complement(19429..20073)
                     /locus_tag="QIE41_001177"
     CDS             complement(19429..20073)
                     /locus_tag="QIE41_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDS3530086.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRNDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            20179..21147
                     /gene="serB"
                     /locus_tag="QIE41_001178"
     CDS             20179..21147
                     /gene="serB"
                     /locus_tag="QIE41_001178"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDS3530087.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            21196..22578
                     /gene="radA"
                     /locus_tag="QIE41_001179"
     CDS             21196..22578
                     /gene="radA"
                     /locus_tag="QIE41_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDS3530088.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            22599..23831
                     /gene="nadR"
                     /locus_tag="QIE41_001180"
     CDS             22599..23831
                     /gene="nadR"
                     /locus_tag="QIE41_001180"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDS3530089.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(24057..24242)
                     /locus_tag="QIE41_001181"
     CDS             complement(24057..24242)
                     /locus_tag="QIE41_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530090.1"
                     /translation="MPLINRIVMPPMTRSRAGDVATDIMADYYAQRASAGLIISEGTQ
                     ISRSAAHNFPRHADLLR"
     gene            complement(24291..24503)
                     /locus_tag="QIE41_001182"
     CDS             complement(24291..24503)
                     /locus_tag="QIE41_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDS3530091.1"
                     /translation="MKTIIAIFTASLFSVAAFNASAQSVTATGTTLDSAEAVIAQKAK
                     EMNASEYKITSARMGNTVTMSAELYK"
     gene            complement(24661..25077)
                     /locus_tag="QIE41_001183"
     CDS             complement(24661..25077)
                     /locus_tag="QIE41_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HDS3530092.1"
                     /translation="MAKTTVSIEIPASADAVWQLMGGFDALPDWLPFIPKSVVTEGGR
                     VRSLTTSDGGTVVERLEAFDNRQRSYTYSIIQAPFPVVDYLSTITVHETADSQVSRVE
                     WFGEFTPVNVTNEEAEALFTGIYRDGLKALKDNFVA"
     gene            complement(25103..25900)
                     /locus_tag="QIE41_001184"
     CDS             complement(25103..25900)
                     /locus_tag="QIE41_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS3530093.1"
                     /translation="MKLDLTGKVAVVSGSTSGIGQGIAAGLAKAGATVVVVGRKQRGV
                     DDAMAAIATQTPTASLRGVVADLSTEDGVAALIAAVPTADVLVNNLGIFNDKDFFSVP
                     DDEWMHFYNVNVLSGVRLARHYAPGMTEQGWGRIIFVSSESGVAIPGDMINYGVTKAA
                     NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGLKEMLADAAAQSGRSAREQADEFVKTS
                     RPSSIIQRAADVEEVANLAVYLASPLSSATTGAALRVDGGVVDTLTM"
     gene            complement(25920..26930)
                     /locus_tag="QIE41_001185"
     CDS             complement(25920..26930)
                     /locus_tag="QIE41_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS3530094.1"
                     /translation="MSIKNLLPGKIGLGGAPLGNMFRAIPEEEAHATVSEAWNLGVRY
                     FDTAPLYGSGLSEIRMGEALSQYPRDEYVLSSKVGRIMLDEMEDPAKRDFGEKGGLFE
                     HGLKNKILNDYSADATLRSIEDSLKRLKTDRLDIVWIHDPAQDFYGDSWLEQFNIART
                     GAFRALTRLRDEGVIKAWGLGVNRVEPCELTLALDEPKPDAFLLAGRYSLLDHERALQ
                     RLMPEALEQNVDIVVGGPYSSGVLAGGEHFEYQKASAAIQQKVAQINAIATRFNVNVK
                     AAALQFALANPAVAAVIPGSSRPGRMAEDLAALNAPVPAEFWAEMRRQNLVAENAPLP
                     TR"
     gene            27064..27951
                     /locus_tag="QIE41_001186"
     CDS             27064..27951
                     /locus_tag="QIE41_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001484212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3530095.1"
                     /translation="MIDLRQLKYFVVVAEEEHVGRAAERLHISQSPLSRQIAQLEERL
                     GLTLFERNQQRIRLTADGQTFLAETRSLLTHANRLESLGKRLGRGEDGGLCIGYIENA
                     MHSGVLSTALRTLRVSRPNVHIALYSQPPATQIEGLRQRSLDFSLVDTPPTDSEQDLE
                     YIQVLDDRMLLALPQNHPLAQEENLVASQLGDEQWIAVIHNDDAHSRDHFVAACVNAG
                     FNPDIRLEAGEPLTALGLVAAGLGLALIQHSLRHNAPEGVVLRELPWMNYSTQLWAAW
                     HKANQRPLVSHFRQILRGE"
     gene            complement(28106..29773)
                     /gene="ettA"
                     /locus_tag="QIE41_001187"
     CDS             complement(28106..29773)
                     /gene="ettA"
                     /locus_tag="QIE41_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDS3530096.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVNNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            29984..31921
                     /gene="sltY"
                     /locus_tag="QIE41_001188"
     CDS             29984..31921
                     /gene="sltY"
                     /locus_tag="QIE41_001188"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDS3530097.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            32011..32337
                     /gene="trpR"
                     /locus_tag="QIE41_001189"
     CDS             32011..32337
                     /gene="trpR"
                     /locus_tag="QIE41_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDS3530098.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(32331..32846)
                     /gene="yjjX"
                     /locus_tag="QIE41_001190"
     CDS             complement(32331..32846)
                     /gene="yjjX"
                     /locus_tag="QIE41_001190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDS3530099.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            32898..33545
                     /gene="gpmB"
                     /locus_tag="QIE41_001191"
     CDS             32898..33545
                     /gene="gpmB"
                     /locus_tag="QIE41_001191"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDS3530100.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(33542..34411)
                     /gene="robA"
                     /locus_tag="QIE41_001192"
     CDS             complement(33542..34411)
                     /gene="robA"
                     /locus_tag="QIE41_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDS3530101.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            34622..35095
                     /gene="creA"
                     /locus_tag="QIE41_001193"
     CDS             34622..35095
                     /gene="creA"
                     /locus_tag="QIE41_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDS3530102.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            35108..35797
                     /gene="creB"
                     /locus_tag="QIE41_001194"
     CDS             35108..35797
                     /gene="creB"
                     /locus_tag="QIE41_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDS3530103.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            35797..37221
                     /gene="creC"
                     /locus_tag="QIE41_001195"
     CDS             35797..37221
                     /gene="creC"
                     /locus_tag="QIE41_001195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDS3530104.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            37279..38631
                     /gene="creD"
                     /locus_tag="QIE41_001196"
     CDS             37279..38631
                     /gene="creD"
                     /locus_tag="QIE41_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDS3530105.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(38691..39407)
                     /gene="arcA"
                     /locus_tag="QIE41_001197"
     CDS             complement(38691..39407)
                     /gene="arcA"
                     /locus_tag="QIE41_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDS3530106.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            39503..39643
                     /locus_tag="QIE41_001198"
     CDS             39503..39643
                     /locus_tag="QIE41_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530107.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
BASE COUNT         9395 a        10156 c        10792 g         9470 t
ORIGIN      
        1 gcattctact ggaagggata tcagttaatt ataaattaaa tgaatgagaa ttctgttttt
       61 cattaagtat ttctaacaac ttaatgtaga tattttaaat gtctccaggc tatttcacca
      121 ggttatcatt tgatgctaac cttctgacac atgcagtgga gttgttgtgc agcaggagta
      181 tgctgatatg aaagtgaatg ctaaggataa tttattcgct taatctatta atttgctgga
      241 aacatttaag gaatgaatat cctgaataaa ccatcgtaat ttttagacag tggatgtgga
      301 ggaaatatgt tgccaggatg ctgcaaaaat ggaattgtta tcagtaaaat acctgttatg
      361 caagcagggt taaaagaggt catgaggact cacttccctg aatatgaaat aatatccagc
      421 gcctctgcgg aggaccttac cttattacaa ttacgtcgtt ccggattagt cattgctgat
      481 ttagccggtg aaagtgaaga tccacgttct gtttgtgaac attattattc tttaatctcg
      541 caatatcggg aaattcactg ggttttcatg gtgtcacgct cctggtattc ccaggcagta
      601 gaactgctta tgtgccctac ggcgacgtta ttgtctgatg ttgaacccat tgagaatctg
      661 gtcaagaccg tacgttccgg caatacgcac gcagagcgta tcagcgccat gctgacctct
      721 ccggcaatga ctgaaactca tgattttagc tatcgctccg tcattctcac tctttcagag
      781 cgcaaggtac tgcggctatt aggtaaagga tggggcatca accagatagc ttcattgctt
      841 aagaaaagta ataaaactat cagcgcccaa aaaaacagtg cgatgcgtcg actggcaatt
      901 cacagcaacg ctgaaatgta tgcatggata aatagcgcgc agggtgcaag agaacttaac
      961 ttgccttctg tttatggaga tgccgcagaa tggaacacag ccgaattaag aagagaaatg
     1021 tcgcactcat agaaaaatgc gtcatgagta gtatcggtat tgagagttta ttcagaaagt
     1081 ttgcgggtaa cccttataag ctccatacct ataccagtca ggagtcattt caggatgcca
     1141 tgtcgcggat ctcgtttgcg gcggtcattt tttctttttc tgccatgaga agtgagcgca
     1201 gagagggatt atcttgcctg actgaactgg cgattaagtt tccgcgtacc cggcgtttag
     1261 ttattgcgga tgatgatatt gaagctcggc tgattggttc attgtcgcca tcaccgttgg
     1321 acggtgtatt aagtaaagcg tcaacgctgg agatttttca tcaggaactc tttttgtcat
     1381 taaatggtgt acgtcaggcg accgaccgac tgaacaatca gtggtacatt aatcaaagcc
     1441 gaacgctaag cccgacggag agagaaatat tgcgctttat gtcgcgtggc tactcaatga
     1501 cacaaattgc cgagcagctt aaacgcaata tcaaaacgat ccgtgcacat aaatttaatg
     1561 tgatgtcgaa actgggcgtc agttctgatg cagggttgtt ggaggccgca gatattctgt
     1621 tatgtatgcg gcattgcgaa gcaagtaatg tgttgcatcc ctattaatcc gcatgatgcc
     1681 gggtttactt ccccggcagt gctttcattt cagcgtacaa tcgccacatt gctgcacatc
     1741 cggtaagcga taacgctggc agcaagtgcg gcgcaccagc aggccgtcgc gcagtaccac
     1801 ggtacgccag agtggattat cttcaccgtt cgtgagcatt ttctcaaaaa agagggcatg
     1861 gcgcagcgat tcaaccgtcg cctcgccaag cagctgcttc atctcagtga gataccagtt
     1921 gatcaaataa ccggtattac tccagataag tttgccgttg atctctcccg tcgcttctaa
     1981 tgcctgcaca accggaacca gcgcctggct gattaacgtt tccattcgct gctgcggcga
     2041 atgtggtgtt gcgtttttat cttcacacac atcgacccag aaacaggcgg cgcgtccggt
     2101 ttcgtgaaac tcaacgtgga aatgttccgg cgacacatct aatgcttttt cctgcgtcag
     2161 cagcgccaac attaatggtg gcaccatcag gccgatatac cattgcgccc atagtgagat
     2221 cagcggtttg ttctcgcgga tcatcgttgg ttggttgcga tagatatgat cggaatagac
     2281 cgcgagtaga gaacttagcg cattcgttga tgaccattgc gccagcgtca tggcgttaag
     2341 cggagcgggt tcatccaggc ggataaactc gagcaaatgt tcacgatgtt cggcgatcgt
     2401 cgcccgtacg gcttgtgcaa gcccagcatc ctgcggctgg agatgcgttc gccagataac
     2461 atcttcatag agcggtgcgg aacgataggc cataatcggg atagtaatct aaatgataat
     2521 gattgctaat cctagcgata ggtttacccg atagcaaggg atttctctgg cttgcaaatg
     2581 ataaaaatta tcatatgata ttggttatca ttatcaatga aagagatgaa accatgttgc
     2641 aacgtacgct gggcagtggc tggggagtgt tgctgccggg attgctaatt gcagggctga
     2701 tgtatgcgga tttatcgccg gatcagtggc ggattgtcat tctgatggga ttagtattga
     2761 cgccggtgat gctgtatcac aaacagttgc ggcattacat tttgctgcca tcgtgcctgg
     2821 cgcttattgc tggcatcatg ctgatgataa tgaatttgaa gcagggatga taaaacaggg
     2881 aagcaacaag aaaggaagta aagataattg gtgcgagggg ggggacttga acccccacgt
     2941 ccgtaaggac actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcacagt
     3001 catcttactt tttttgatat cgcctcgttt ggtgcgaggg gggggacttg aacccccacg
     3061 tccgtaagaa cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcccag
     3121 tgcgagcaat atcaacgtgg tttttggtgc gagggggggg acttgaaccc ccacgtccgt
     3181 aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg cataccatca
     3241 attcttaaaa agaattgcta ccacggaggc gcattctagt ggttttcagc ttttcgtcaa
     3301 tagttaatta tcgccggagg tgtaattgct ggaaaaatgt ccatcagcga attgtggata
     3361 attcgtttgt cggtcagaga aggcgtgtac gccgcatccg acacggtatg cggcgacaga
     3421 tatcagatac tcaacctttc ttcgcctggc gggtcataat ggcgcgatac accttgaagc
     3481 gcccggtttg cgcgatcact tcgtggaagc caaatgtctc atccagcacg tccgggtaag
     3541 gcaggaaggc gttcgctaca attcgcagct cgccgccgct attaagatga cgcaccgcgc
     3601 cgcgaatcag cgtttgcgcc gcatccaggc tggtttgcat cccatcgtgg aacggcgggt
     3661 tggagatgat catatcaaaa cgacctttca cctcggaaaa gacgttactg gcaaagactt
     3721 caccttcaac accgttggcc gcaagtgttg cgcggctggc ttctaccgct ggcgcagaga
     3781 catcgcacag agtgagacga attttcggcg aatggcgcgc aaaggcaaca gaaagcacgc
     3841 ccgcgccaca gccgacatcc agcactttac ctttcgtgtg cggagttaac gtcgagagca
     3901 gtaactggct accgacatcc agaccgtcgc ggctaaacac gcccggcagc gttttgaccg
     3961 tcaggccatc cacgttatat tcgccccaga atttatccgc atcaaattcc ggctgttttt
     4021 ccagacgacc aaaatagagg ccgcagcgac gggcgctgtc gactttattc aacggcgcat
     4081 aatctgccag catctgttcg gcgctgcgca cgccgctgcg gttctcgcca acgacaaaaa
     4141 tatccgtgcc caccggcagc agagaaagta aattcatcaa ctggaactgg gcttccggtt
     4201 tattcttcgg ccagtagtag atcagcgtat cgcaatctgc gacgtcatct gccgttgcga
     4261 ccagactaaa acgggcgtta tcccccatct ggcggcttaa cacctgccaa tggtggaatt
     4321 gctgggtatg agcacggctg gccgcggtat ctaaacgcgc gggcaggtca tcctgtaagt
     4381 ctccggcaaa cagaatacgg ctttgttcga aatcatcact gtgacgcagc aagacttcac
     4441 ttgccggggt aaatgcagac atggaatgct cctcaattga tactggcggc gattatagcc
     4501 atatgttggc gcggtatcga cgaatttgct atatttgcgc ccctgacaac aggagcgatt
     4561 cgctatgaca tcccgacgag actggcagtt acagcaactg ggcattaccc agtggtcgct
     4621 gcgtcgccct ggcgcgttgc agggggagat tgccattgcg atcccggcac acgtccgtct
     4681 ggtgatggtg gcaaacgatc ttcccgccct gactgatcct ttagtgagcg atgttctgcg
     4741 cgcattaacc gtcagccccg atcaagtgct gcaactgacg ccagaaaaaa tctcgatgct
     4801 gccacaaggc agtcgctgca acagctggcg gttgggtact gacgaaccgc tatcactgga
     4861 aggcgctcag gtggcatcac cggcgctcac cgaattacgg gcaaacccaa tggcacgcgc
     4921 cgcgttatgg caacaaattt gcacatatga acacgatttc ttccctcgaa acgactgatt
     4981 taccagcggc ttaccacatt gaacaacgcg cccacgcctt tccgtggagt gagaaaacct
     5041 ttgccagcaa ccagggcgag cgttatctca actttcagtt aacgcaaaac ggcaaaatgg
     5101 cggcgtttgc gattacgcaa gtggtgctgg atgaagctac attgttcaat attgcggtcg
     5161 atcctgacta tcagcgtcag ggattgggaa gggcgctgct ggaacatctg atcgacgaac
     5221 tggaaaaacg cggcgtggcg acgctatggc tggaagtccg tgcctcaaac gctgccgcca
     5281 ttgcactgta cgaaagttta ggctttaacg aggcgacgat tcgccgcaat tactacccca
     5341 ccacggacgg tcgcgaagac gccatcatca tggcgttgcc aatcagtatg taagacaagg
     5401 tggaataatg aagtgggact ggattttctt tgatgccgat gaaacgctgt ttacctttga
     5461 ctcattcacc ggcctgcagc ggatgtttct tgattacagc gtcaccttta ccgctgaaga
     5521 ttttcaggac tatcaggccg ttaacaagcc actgtgggtg gattatcaaa acggcgcgat
     5581 cacttcatta cagcttcagc acgggcggtt tgagagctgg gccgaacggc tgaacgtcga
     5641 gccgggtaaa ctcaacgaag cctttattaa tgcgatggcg gaaatctgta cgccgctgcc
     5701 gggcgcggtt tctctgctta acgccattcg tggcaacgct aaaattggca tcatcaccaa
     5761 cggctttagc gccttgcagc aagtgcgtct ggaacgcacg ggcctgcgtg attatttcga
     5821 tttgctggtg atttccgaag aagttggcgt tgccaaaccg aataagaaaa ttttcgatta
     5881 tgcgctggaa caggcgggca atcctgaccg ttcacgcgtg ctgatggttg gcgacactgc
     5941 cgagtccgat attctcggtg gcatcaacgc cgggcttgcg acctgctggc tgaatgcgca
     6001 caatcgcgag caaccagaag gcatcgcgcc tacctggacc gtttcttcgt tgcacgaact
     6061 ggagcagctc ctgtgtaaac actgattgcc tcccccccgt tgatgggtaa aatagccgca
     6121 atttttcgtt ttcaacaagc gcggcgcgat gccgcttact caagaagaaa gaattatgac
     6181 gttgtctcct tatttgcaag aggtggcgaa gcgccgcact tttgccatta tttctcaccc
     6241 agacgccggt aagactacca tcaccgagaa ggtgctgctg ttcggacagg ccattcagac
     6301 cgccggtaca gtaaaaggcc gtggttccaa ccagcacgct aagtcggact ggatggagat
     6361 ggaaaagcag cgtgggatct ccattactac gtctgtgatg cagtttccgt atcacgattg
     6421 cctggttaac ctgctcgaca ccccggggca cgaagacttc tcggaagata cctatcgtac
     6481 cctgacggcg gtggactgct gcctgatggt tatcgacgcc gcaaaaggtg ttgaagatcg
     6541 tacccgcaag ctgatggaag ttacccgtct gcgcgatacg ccgatcctca cctttatgaa
     6601 caaacttgac cgtgatatcc gcgacccgat ggaactgctc gatgaagttg agaacgagct
     6661 gaaaatcggc tgcgcaccga tcacctggcc gattggctgc ggcaagctgt ttaaaggcgt
     6721 ttaccacctt tataaagatg aaacctatct ttatcagagc ggtaaaggcc acaccattca
     6781 ggaagtccgt attgttaaag ggctgaataa cccggatctc gacgctgcgg ttggtgaaga
     6841 tctggcgcag cagctgcgtg atgaactgga gctggtgaaa ggcgcgtcta acgagttcga
     6901 caaagaattg ttccttgcgg gcgaaatcac tccggtattc tttggtactg cgctgggtaa
     6961 cttcggcgtc gatcatatgc tggatggtct ggtggagtgg gcacctgcgc cgatgccgcg
     7021 tcagactgat acccgtaccg tagaagcgag cgaagacaaa tttaccggct tcgtatttaa
     7081 aattcaggcc aacatggacc cgaaacaccg cgaccgcgtg gcgtttatgc gcgtggtatc
     7141 cggtaaatat gaaaaaggca tgaaactgcg ccaggtgcgc accgcgaaag atgtggtgat
     7201 ctccgacgcg ctgaccttta tggcgggcga ccgttcgcac gttgaagaag cgtatccggg
     7261 agacatcctc ggcctgcaca accacggcac cattcagatc ggcgacacct ttacccaggg
     7321 tgagatgatg aagttcaccg gtattccgaa cttcgcacca gaactgttcc gtcgtatccg
     7381 cctgaaagat ccactgaagc aaaaacagct gctcaaaggg ctggtacagc tttccgaaga
     7441 gggcgcagtg caggtgttcc gtccgatctc caacaacgac ctgatcgttg gtgcagttgg
     7501 tgtgctgcag tttgatgtgg tggtagcgcg cctgaagagc gaatacaacg ttgaagcagt
     7561 gtatgagtca gtcaacgttg ccactgcacg ctgggtagaa tgtgcggacg cgaagaaatt
     7621 cgaagagttc aagcgtaaga acgaaagcca actggcgctt gatggcggcg ataacctcgc
     7681 ttacatcgct accagcatgg tcaacctgcg tctggcgcag gaacgttatc cggacgttca
     7741 gttccaccag acccgcgagc attaattctt gcctccaggg cgcggtagcc gctgcgccct
     7801 gtcaatttcc cttccttatt agccgcttac ggaatgttct taaaacattc acttttgctt
     7861 atgttttcgc tgatatcccg agcggtttca aaattgtgat ctatatttaa caaagtaatg
     7921 acatttctga cggcgttaaa taccgttcaa tgcgtagata tcagtatcta aagccgtcga
     7981 ttgtcattct accgatatta ataactgatt cagaggctgt aatggtcgtt attcatcacc
     8041 catcgctttt gtgatggcga ccattgactt ctgtagaggg tgaagtctct ccctattcag
     8101 caatgcaacc tcgtgttgcc aggctcaaat tacgagcaaa catacaggaa taaatcgatg
     8161 actatgacaa gactgaagat ttcgaaaact ctgctggctg taatgttgac ctctgccgtc
     8221 gcgaccggct ctgcctacgc ggaaaacaac gcgcagacta ccaatgaaag cgcagggcaa
     8281 aaagtcgata gctctatgaa taaagtcggt aatttcatgg atgacagcgc catcaccgcg
     8341 aaagtgaagg cggcgctggt ggatcatgac aacattaaga gcaccgatat ctctgtcaaa
     8401 accgatcaaa aagtcgtgac cctgagcggt ttcgttgaaa gccaggctca ggccgaagag
     8461 gcagtgaaag tggcgaaagg cgttgaaggg gtgacctctg tcagcgacaa actgcacgtt
     8521 cgcgacgcta aagaaggctc ggtgaagggc tacgcgggtg acaccgccac caccagtgaa
     8581 atcaaagcca aactgctggc ggacgatatc gtcccttccc gtcatgtgaa agttgaaacc
     8641 accgacggcg tggttcagct ctccggtacc gtcgattccc aggcacagag cgaccgtgct
     8701 gaaagtatcg ccaaagcggt agatggtgtg aaaagcgtta aaaatgatct gaaaactaag
     8761 taattcgtcg taattcgtcc tcccgaactt tgtcgggagg cgcgatgtgc accacactaa
     8821 aaatatcgca ttgagtgatt ttcacgctca tatcaagcgg tgacattaac tatggtaaag
     8881 gagacgctta tgtttcgttg gggcatcata tttctggtta tcgcgttaat cgccgccgca
     8941 cttgggtttg gtggtctggc cggtaccgct gcaggcgcag ctaaaattgt ctttgtcgtc
     9001 gggattattc tgttcctggt gagtttgttc atgggccgaa aacgacccta gatttcagac
     9061 cattaagaaa attttatcca aagccagtcc agcggactgg cttttgcggt tttagcgaat
     9121 aataaattgc gttactttgt cattctttca caacaaaaac aggaaggcag aggtggggca
     9181 gcgaatacct gtaacgcttg gtaatattgc gccgttgtcg ctaaggccgt tccagcctgg
     9241 acgaatagct ctggtgtgcg aaggcggcgg acagcgtgga attttcacgg ctggcgtgct
     9301 ggatgagttt atgcgcgcgc agtttaatcc tttcgatctt tatctcggca catctgccgg
     9361 ggcgcagaac ctctcggcgt atatctgcaa tcagcccggt tacgcgcgca aagtcatcat
     9421 gcgctatacc acaaaacgcg aatttttcga tccattgcgc tttgtccgtg gaggaaatct
     9481 tatcgatctc gactggctgg tggaggccac tgcaagccag atgccgttgc aaatggatac
     9541 cgccgcgcgg ttgtttgaca gcggcaaatc gttttatatg tgcgcctgtc gtcaggatga
     9601 ctatgcgccg aattactttt taccaaccaa acaaaactgg ctggatgtga ttcgcgcctc
     9661 cagtgcgata cctggctttt atcgtagcgg agtgtcgctg gaaggcatta actacctgga
     9721 tggcgggatc agtgatgcga ttccggttaa agaggcggca aggcagggag caaaaacctt
     9781 ggtcgtcatc cgcaccgtgc cgtcgcaaat gtactacacg ccgcagtggt tcaaacgcat
     9841 ggaacgctgg ctgggtgaca gtagcctgca gccgctggtc aatctggtgc agcatcatga
     9901 aaccagctat tgtgatattc agcaatttat tgagaaacca ccgggcaaac tgcggatatt
     9961 cgaaatttat ccgccgaagc cattgcatag tatcgcgctt ggcagtcgga ttccggcgct
    10021 gcgtgaagac tataaacttg ggcgtttatg cggtcgttat ttcctggcaa cggttggcaa
    10081 gctattaact gaaaaagcgc cgctaacccg ccatctggtg ccagtggtga cgccggaatc
    10141 gattgtcatt ccgcctgcgc cagtcgccaa cgatacgctg gttgccgaag tgagcgatgc
    10201 tccgcaggcg aacgacccga catttaacaa tgaggatctg gcttgatttg ccgttttatc
    10261 gacacccact gccattttga tttcccgccg tttagtggcg atgaagaggc cagcctgcaa
    10321 cgcgcggcac aagcgggcgt aggcaagatc attgttccgg cgacagaggc ggaaaatttt
    10381 gcccgcgtgc aggcgttagc ggaaaaatat caaccgctgt atgccgcatt gggcttgcat
    10441 cctggtatgt tggaaaaaca tagcgatgtg tctcttgatc agctacagca ggcgctggaa
    10501 aggcgtccgg cgaaggtggt ggcggtgggg gagatcggtc tggatctctt cggcgacgat
    10561 ccgcaatttg agaggcagca gtggttactc gacgaacaac tgaaactggc gaaacgctac
    10621 gatctgccgg tgatccttca ttcacggcgc acgcacgaca aactcgcgat gcatcttaaa
    10681 cgccacgatt tgccgcgtac tggcgtggtt cacggttttt ccggcagcct gcaacaggct
    10741 gaacggtttg tacagctggg ctacaaaatt ggcgtaggcg gcactatcac ctatccacgc
    10801 gccagtaaaa cccgcgatgt catcgcaaaa ctaccgctgg catcgttgtt gctggaaacc
    10861 gacgcgccgg atatgccgct caacggcttt caggggaagc ctaatcgccc ggaacaggcc
    10921 gcgcgggtgt ttgccgtgct ttgcgagtta cgcccggaac ccgcggatga aattgccgaa
    10981 gtgttgctta ataacacgta tacgttgttt aacgtgccgt aggccggaca aggcgtttac
    11041 gccacatccg gcatgtcaca aatacagcgc cggaaatatc aaccggctca ccccgcgcac
    11101 ctttaacgca tcagccaact gctcaacatc ttccggtgtg gcgctcggcc agctttgcgc
    11161 ttcgccatac acgccgtggg catgaaacgc gttcaggcgt accggaacat cgccaagtac
    11221 cttgataaac gccgccagtt cttcgatgtg ttgcaaataa tccacctggc cagggattac
    11281 cagcaaacgc agttccgcca gcttgccgcg ctctgccagc aaatagatgc tgcgcttaat
    11341 ctgctgatta tcgcgtccgg ttaactgttg atgacattcg ctcccccacg ctttgagatc
    11401 gagcatcgcg ccgtcgcaca ccgggagcaa tttttcccag ccggtttcgc tcaacatacc
    11461 gttactgtcc accagacagg tgagatggca cagttgcgga tcatttttga tagcagtaaa
    11521 cagcgccacc acaaacggta gctgggtcgt ggcttcgccg ccgcttaccg ttatcccttc
    11581 gataaacagc actgctttgc ggatatggct aagcacctca tccacgctca tggactgcgc
    11641 cattggcgtg gcatgttgcg gacacatctt caggcaggta tcacattgct cgcagaccac
    11701 agcgttccac accactttgc cgtcaacaat ctgcaacgcc tgatgcggac actgtggcac
    11761 gcactccccg cagtcattgc aacgtcccat cgtccacgga ttgtgacagt ttttgcagcg
    11821 cagattgcag ccctgcaaaa acagagccag acgactgcct ggcccgtcaa cgcaggagaa
    11881 ggggataatc ttactgacta aagcgcatct gctgttcatg gcttatcacg cgcggctggc
    11941 gttccagaat acgagtgtta cgtgcggcct cttcgcccag ccaggtggtg ttggtacgtg
    12001 aaccttcggc gcgatatttt tctaaatccg acaaacgcac catataaccg gtaacgcgaa
    12061 ccagatcgtt accgctgaca ttggcggtaa attcacgcat tccagcttta aaggcaccga
    12121 ggcaaagctg taccagcgcc tgcggattac gtttgatggt ttcgtcgagc gtcagaatgt
    12181 cgctgatgcc ggaataataa taagcatgat gcggggcgac ggtttgcaga tgggtgatcg
    12241 gatctggctc atcaccatac ggcaaacgtg cgcccggcgt ggtgccgata tcggaactta
    12301 tccctgactg tgcgtgtaac atggcgcgtt tttgccagcc atatttcacg ggggtattgg
    12361 cgacaaactc cgccagttgc gcgctgatgc gataacccac ttcatttgcg gcggcttctt
    12421 taccgtagcg cgcggcaatc ccttctttct cacacagcaa gttaaccgct tccgccagcc
    12481 catacatgcc aaacattggc acaaaacgtt cagggttaat cagcccttct ttcaccagga
    12541 agctattctc aaagaagtgt gattgttgat agaggaattc acaccgcgca tcgatgatgg
    12601 cgatctgctg ctggcagtag tgcggtagag tgcgcgcaaa gaagtcatcc agcgattcgc
    12661 tgcgctcggc aatggctttc aggttaaggc gtaccagcgt gctgccacca cccgccagcg
    12721 gcagtgagtt gtaacagctc acaatcccgt agcccccttt tgtgaaaatt ttatcatgca
    12781 ccggaccgtt ggcgatgtgc ggtttgctac attcacagat gttcttcgcc acttcctgca
    12841 gcaggtcatc aggggtgatt tcaggatcgt agataaaggt caggttcggt gaaacctgct
    12901 tcagctctgc atctgcacgt aagattgcac gggtgatggg cgaatcagac gggccgatat
    12961 tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta acgccagaaa cgttttattc
    13021 gaatatcgat ctcgtcttgt gttagaattc taacatacgg ttgcaacaac gcatccagtt
    13081 gccccaggta gaccggcatc gatgtgaccg acggtacgtg gtggtaaaga atggtcagca
    13141 gagagagtgc atcatcaaga tctttcgcgc cttccagctc cagccattcg gaaccgttcg
    13201 ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt gtacggcgca tgaccttcaa
    13261 acatatcgca gattacaccc tcatccagcg cgcggcgggc ttcggcagga agctgcggat
    13321 aaggcagatt gttttctgct tccagcgcca gaaaatggcg cttctgctcc gggctaagta
    13381 ccggactggt gacaatttgc tggcaacgtt gttgcagtgc aatctcatga gaagtgggca
    13441 tcttcttttc cttttatgcc gaaggtgatg cgccattgta agaagtttcg tgatgttcac
    13501 tttgatcctg atgcgtttgc caccactgac gcattcattt gaaagtgaat tatttgaacc
    13561 agatcgcatt acagtgatgc aaacttgtaa gtagatttcc ttaattgtga tgtatatcga
    13621 agtgtgttgc ggagtagatg ttagaatact aacaaactcg caaggtgaat tttattggcg
    13681 acaagccagg agaatgaaat gactgatctg aaagcaagca gcctgcgtgc actgaaattg
    13741 atggacctga ccaccctgaa tgacgacgac accgacgaga aagtaattgc tctgtgtcat
    13801 caggccaaaa ctccggtcgg caataccgcc gctatctgta tctatcctcg ctttatccca
    13861 attgctcgca aaacgctgaa agagcagggt accccggaaa tccgtatcgc tacggtaacc
    13921 aacttcccac acggtaacga cgacatcgac atcgcgctgg cagaaacccg tgcggcaatc
    13981 gcctacggtg ccgatgaagt tgacgtagtg ttcccgtacc gtgcgctgat ggcaggtaac
    14041 gagcaggttg gttttgacct ggtgaaagcc tgtaaagatg cttgtgcggc agcgaatgta
    14101 ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg aagcgctgat ccgtaaagcg
    14161 tctgaaatct ccatcaaagc gggtgctgac ttcatcaaaa cctctaccgg taaagtggca
    14221 gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag tgatccgtga tatgggcgta
    14281 gaaaaaaccg ttggtttcaa accggcgggc ggcgtgcgta ctgcggaaga tgcgcagaaa
    14341 tatctcgcca tcgccgatga actgttcggc gctgattggg ccgatgcgcg tcactaccgc
    14401 tttggcgctt ccagcctgct ggcaagcctg ctgaaggctc tgggccacgg cgacggtaag
    14461 agcgccagca gctactaagg cttgatttgc cggatgacgc agcgatgctc atccggttcc
    14521 ccgattacct cgtctctttt ccacgggagg ttaccttgtt tctcgcacaa gaaattattc
    14581 gtaaaaaacg tgatggtcat gcgctgagcg atgaagaaat tcgtttcttt atcaacggta
    14641 ttcgcgacaa cactatctcc gaagggcaga ttgccgctct cgcgatgacc attttcttcc
    14701 acgatatgac aatgcctgag cgtgtctcgc tgaccatggc gatgcgagat tcaggaaccg
    14761 ttctcgactg gaaaagcctg catctgaatg gcccaattgt tgataaacac tccaccggtg
    14821 gcgtcggcga tgtgacttcg ctgatgttgg ggccgatggt cgcagcctgc ggcggctata
    14881 ttccgatgat ctccggtcgc ggcctcggtc atactggcgg tacgctcgac aaactggaat
    14941 ccatccctgg cttcgacatt ttcccggatg acaaccgttt ccgcgaaatt attaaagacg
    15001 tcggcgtggc gattatcggc cagaccagct cactggctcc ggcggataaa cgtttctacg
    15061 cgacccgtga tattaccgca accgtggact ccatcccgct gatcaccgcc tctattctgg
    15121 cgaagaaact ggcggaaggt ctggacgcgc tggtgatgga cgtgaaagtg ggtagcggcg
    15181 cgtttatgcc gacctacgaa ctctctgaag cccttgccga agcgattgtt ggcgtggcta
    15241 acggcgctgg cgtgcgcacc accgcgctgc tcaccgatat gaatcaggta ctggcctcca
    15301 gtgcaggtaa cgcggttgaa gttcgtgaag cggtgcagtt cctgacgggt gagtatcgta
    15361 acccgcgtct gtttgatgtc acgatggcgc tgtgcgtgga gatgcttacc tccggcaaac
    15421 tggcgaaaga tgacgccgaa gcgcgcgcga aattgcaggc ggtgctggac aacggtaaag
    15481 cggcagaagt ctttggccgt atggtggcgg cacaaaaagg cccgaccgac ttcgttgaga
    15541 actacgcgaa gtatctgccg acagcgatgc tgacgaaagc agtctatgct gataccgaag
    15601 gttttgtcag tgaaatggat acccgtgcgc tggggatggc agtggttgca atgggcggcg
    15661 gacgccgtca ggcatctgac accatcgatt acagcgtcgg ctttactgat atggcgcgtc
    15721 tgggcgacca ggtagacggt cagcgtccgc tggcggttat ccacgcgaaa gacgaaaaca
    15781 gctggcagga agcggctaaa gcggtgaaag cggcaattaa acttgccgat aaagcaccgg
    15841 aaagcacacc aactgtctat cgtcgtatca gtgaataacg gtatactgat ctgatcattt
    15901 aaatttgaag cactgagtac ggagaacata tgaaacgtgc atttattatg gtgctggact
    15961 cattcggcat cggcgctaca gaagatgcag aacgctttgg tgacgtcggg gctgacaccc
    16021 tgggtcatat cgcagaagct tgtgccaaag gcgaagctga taacggtcgt aagggcccgc
    16081 tcaatctgcc aaatctgacc cgtctggggc tggcgaaagc acacgaaggt tctaccggtt
    16141 tcattccggc gggaatggac ggcaacgctg aagttatcgg cgcgtacgca tgggcgcacg
    16201 aaatgtcatc cggtaaagat accccgtctg gtcactggga aatcgccggt gtgccggttc
    16261 tgtttgagtg gggatacttc tccgatcacg aaaacagctt cccgcaagag ctgctggata
    16321 aactggtcga acgcgctaat ctgccgggtt acctcggtaa ctgccactct tccggtacgg
    16381 tcattctgga tcagctgggc gaagagcaca tgaaaaccgg caagccgatt ttctatacct
    16441 ccgctgactc cgtgttccag attgcctgcc atgaagaaac tttcggtctg gataaactct
    16501 acgaactgtg cgaaatcgcc cgtgaagagc tgaccaacgg cggctacaac atcggtcgtg
    16561 ttatcgctcg tccgtttatc ggcgacaaag ccggtaactt ccagcgtacc ggtaaccgtc
    16621 acgacctggc cgttgaaccg ccagcaccga ccgtgctgca gaaactggtt gatgaaaaac
    16681 acggccaggt ggtttctgtc ggtaaaattg cagacatcta cgccaactgc ggtatcacca
    16741 aaaaagtgaa agcgactggc ctggacgcgc tgtttgacgc caccatcaaa gagatgaaag
    16801 aagcgggtga taacaccatc gtcttcacca acttcgttga cttcgactct tcctggggcc
    16861 accgtcgcga cgtcgccggt tatgctgcgg gtctggaact gttcgaccgc cgtctgccgg
    16921 agctgatgtc tctgctgcgc gatgacgaca tcctgatcct caccgctgac cacggctgtg
    16981 atccgacctg gaccggtact gaccacacgc gtgaacacat tccggtactg gtttacggcc
    17041 cgaaagtaaa accgggctca ctgggtcacc gtgaaacctt cgcggatatc ggccagactc
    17101 tggcaaaata ttttggtact tctgatatgg aatatggcaa agccatgttc tgatggattt
    17161 gggcggagcg ttgactccgc ctttgttatg tcacaaaaag gataaaacaa tggctacccc
    17221 acacattaat gcagaaatgg gcgatttcgc tgacgtagtt ttgatgccag gcgacccgct
    17281 gcgtgcgaag tatattgctg aaactttcct tgaagatgcc cgtgaagtga acaacgttcg
    17341 cggtatgctg ggcttcaccg gtacttacaa aggccgcaaa atttccgtaa tgggtcacgg
    17401 tatgggtatc ccgtcctgct ccatctacac caaagaactg atcaccgatt tcggcgtgaa
    17461 gaaaattatc cgcgtaggct cctgtggcgc tgttctgcca cacgtaaaac tgcgcgacgt
    17521 cgttatcggt atgggtgcct gcaccgattc caaagttaac cgcatccgtt ttaaagacca
    17581 tgactttgcc gctatcgctg actttgacat ggtgcgtaac gcagtagatg cagctaaagc
    17641 actgggcatt gatgctcgcg ttggtaacct gttctccgct gacctgttct actctccgga
    17701 cggcgaaatg ttcgacgtga tggaaaaata cggcatcctc ggcgtggaaa tggaagcggc
    17761 tggtatctac ggcgtcgctg cagaatttgg cgcgaaagcc ctgaccatct gcaccgtgtc
    17821 tgaccacatc cgtactcacg agcagaccac tgccgctgag cgtcagacca ccttcaacga
    17881 catgatcaaa atcgcactgg aatccgttct gctgggcgat aaagagtaat tgtgtttcgc
    17941 tgaaaggcga ttgccttgtg aagccggagc gggagactgc tccggctttt tagtatctat
    18001 tcatcaatag caactgattt atagatttac tattattttt catatggata gaaaataaaa
    18061 tacattcaag tatctattca cgaatctatt catttatgtt cagccgtcat ccaggaaggg
    18121 catatcagca actctggtgg acaaccataa attcctgcca ttttgctttt gatgtttgaa
    18181 ctaagccgtt ccgtcttttc gatctgtttt agagaattaa tggagatacc taacatttta
    18241 cttacttcag caagtgatag tttatgcctg atacgcacgg cagttatcca ggatataccc
    18301 tggctgaaca ttaaattgat aatctcccgt tcctggtttt ccagatcgat catatttcct
    18361 tccctggata ttttaatcag cgagttacct taccgcccca gccatccatg ccgataactc
    18421 ccgtagctct ttttcctgtt ccgggaagtc aaccaacagc gcttcgcact cctgttgcag
    18481 catatctgcg cggtacaggc agccttgcag tcgcccggcg agggcttcca gcggcgcggg
    18541 gttgaggctg tcggtaaaca cctgggcgcg ggtgatatgg cctttttcaa cgtcgaaatg
    18601 cagttccaca ccgccccagg taaagcgttc atccagcaga tgcgagaatg ccggagcctg
    18661 accgaagttc cattcccagc tactctggcg ggcaaaggtt tcggcgaagt tcggcaggtc
    18721 tggcgttttg tccggggaga tgatttccgc ttccacgcgc tcgccataat gggcgaaaaa
    18781 ggcctcggtt atggcctcgc aaacctgctc atgggtaatt tccggcagca attcggtgag
    18841 gttggtcacg cgggaacgta ccgacgtaat gcctttcgcc gccagtttct ttttatccgg
    18901 attgagatag tttgccaggc ggctaaggtc ggcattgagc agcaaggtgc cgtggtggaa
    18961 gccacgatct ttggtttcgc gataggccga gcctgagact ttgcggtcgc cttcggcggt
    19021 tttcaccacc agatcgttac gtccggacgc ttcggcgctg acgccgagcg cgttcagtgc
    19081 attgagcaca atcgacgtgg agatggtttt atcgtactcc ggcttgccag ccataaaggt
    19141 aaaacaggta ttgccgagat cgtggaacac cgcgccgcca ccgctactgc gccgcgccag
    19201 gcggacgtta tcttcttcca tccgccgggt attacactct ttccacgggt tctgtgcgcg
    19261 accaatcact accgtgtcgg cattgcgcca gagaaacaga acgcgctgcg tggcgggcat
    19321 ctggcgaaaa atacactcct ccaccgccag gttaaaccac gggtcgtaag agtcagagat
    19381 gagcaggcgt aatgtggaca taacgatttc ctttcttgta gtaatgggtc actctttttt
    19441 ctcgctttct tcctcttccg gcaccggttt gctggcggtt aacaggaagg gcgattgctg
    19501 ccagcgggtg cgtttaccct gtagcagcgt gcgggtcagt actacgccga ttgccagtga
    19561 gagcagcaac atcaggcgta aaatgttagt ggtgttatcc acctgttggg cttcggtggc
    19621 gagcgtatgg gtgtcgagcg tcaggcgcag atagccgagc ggtccgtttt tcccggcaat
    19681 aggttcgaca atctgctggt taaaatagcc gcccgctttt ttgccgtcga gcgccaaccg
    19741 gtcgcgcact tcgacgcttt cgccagaacg ggcgataaga tcgccttgtt cgtcatacac
    19801 gcccgcgtcg aggatgcggc tttcatccgt taactgatcg aggatcgcct gaatgcgttt
    19861 ttcatccggt gagtcgttac gcatcagcgg tgcaacgttc agcgtcacct gacgcgccag
    19921 ggtgcgggcc agttcttcca gctgtggatt acgctgtcgc tggtggtttt gactaaacca
    19981 tgacgctccc tgcatcagcg ccactaacaa ggcaagacag aacaggacaa tcactgcccg
    20041 atgcagccgg aatttcagtt ttgtgcgagc catcttccac cctttgaaaa tttgagactt
    20101 aatgttgcca gaagcaatgg atacaaggta gcctcatgcg ttattttccc tgcttcgaac
    20161 gattttacag gagccttaat gcctaacatt acctggtgcg acctgcctga agatgtctct
    20221 ttatggccgg gtctgcctct ttcattaagt ggtgatgaag tgatgccact ggattaccac
    20281 gcaggtcgta gcggctggct gctgtatggt cgtgggctgg ataaacagcg tctgacccaa
    20341 taccagagca aactgggcgc ggcgatggtg attgttgccg cctggtgcgt ggaagattat
    20401 caggtgattc gtctggcagg ttcactcacc gcgcgggcta cgcgcctggc ccacgaagcg
    20461 cagctggatg tcgcgccgct ggggaaaatc ccgcacctgc gcacgccggg tttgctggtg
    20521 atggacatgg attccaccgc catccagatt gaatgtattg atgaaattgc caaactggcc
    20581 ggaacgggcg agatggtggc ggaggtaacc gaacgggcga tgcgcggcga actcgatttt
    20641 accgccagcc tgcgcagccg cgtggcgacg ctgaaaggcg ctgatgccaa tattctgcaa
    20701 caggtgcgtg aaaatctgcc gctgatgcca ggcttaacgc aactggtgct caagctggaa
    20761 acgctgggct ggaaagtggc gattgcttcc ggcggcttta ctttctttgc tgaatatctg
    20821 cgcgacaagc tgcgcctgac agccgtggta gccaatgaac tggagatcat ggatggtaaa
    20881 tttaccggca atgtgatcgg cgacatcgtt gacgcgcagt acaaagcgaa aactctgact
    20941 cgcctcgcgc aggagtatga aatcccgctg gcgcagaccg tggcgattgg tgatggagcc
    21001 aatgacctgc cgatgatcaa agcggcaggg ctggggattg cctaccatgc caagccaaaa
    21061 gtgaatgaaa agacggaagt caccatccgt cacgctgacc tgatgggggt attctgcatc
    21121 ctctctggca gcctgaatca gaagtaattg ctcgcccgtc atcctgcggg cggcacagca
    21181 ttaacgaggt acaccgtggc aaaagctcca aaacgcgcct ttgtttgtaa tgaatgcggg
    21241 gccgattatc cgcgctggca ggggcagtgc agcgcctgtc atgcctggaa caccatcacc
    21301 gaggtgcgtc ttgcagcgtc gccaacggtg gcgcgtaacg agcgtctcag tggctatgcc
    21361 ggtagcgccg gggtggcaaa agtccagaaa ctctccgata tcagccttga agagctgccg
    21421 cgtttttcca ccggatttaa agagttcgac cgcgtactag gcggtggcgt ggtgccggga
    21481 agtgccattc tgattggcgg taacccaggt gccgggaaat ccacattgct attgcaaacg
    21541 ctgtgcaaac tggcccagca gatgaaaacg ctgtatgtca ctggcgaaga gtcgctgcaa
    21601 caggtggcaa tgcgcgctca tcgccttggc ctgccgaccg acaatctcaa tatgttgtcg
    21661 gaaaccagca tcgagcagat ctgcctgatt gccgaagaag agcaaccgaa gctgatggta
    21721 attgactcca tccaggtgat gcatatggcg gatgttcagt catcgcctgg cagtgtggcg
    21781 caggtgcgtg aaacggcggc ttatctgacg cgcttcgcca aaacgcgcgg cgtggcgatt
    21841 gtcatggttg ggcacgtaac caaagatggc tcgctggctg gccctaaagt gctggaacac
    21901 tgtatcgact gttcggtgct tctggatggc gatgccgact cccgttttcg caccttacgc
    21961 agccataaaa accgcttcgg cgcggtgaat gagctgggcg tcttcgcgat gaccgaacag
    22021 gggctgcgtg aagtcagcaa cccttctgcc atctttttaa gccgcggtga tgaagtgacc
    22081 tccggtagct cagtgatggt agtgtgggaa ggaacgcgtc cgctgctggt ggagatccag
    22141 gcgctggtcg atcactcgat gatggcgaat ccacgccgcg tcgcagtagg gctggagcaa
    22201 aaccgtctgg caatcctgct ggcggtgctg caccgtcacg gtgggttgca aatggccgat
    22261 caggatgtat ttgttaacgt ggtcggcggc gtgaaggtga cggaaaccag cgccgactta
    22321 gcgttactgc tggcgatggt ttccagcctg cgcgacagac cgctgccaca ggatctggtg
    22381 gtgtttggtg aagtcgggct ggcaggggag atccgcccgg tgcccagcgg ccaggaacga
    22441 atctctgaag cggcgaaaca cggttttcgc cgggcgattg ttccggcggc taatgtgccg
    22501 aaaaaagcgc cggaagggat gcagattttt ggcgttaaaa aactctccga cgcgcttagc
    22561 gtgttcgacg acttataatg agagatacgg agggagatat gtcgtcattt gattacctga
    22621 aaactgccat caagcaacag ggctgcacgc tacagcaggt agctgatgcc agcggtatga
    22681 ccaaagggta tttaagccag ttactgaatg ccaaaattaa aagccccagc gcgcaaaagc
    22741 tggaggcgtt gcaccgtttt ttggggcttg aatttccccg gcagaagaaa accattggtg
    22801 tcgtattcgg taagttctac ccgctgcata ccggacatat ctaccttatc cagcgcgcct
    22861 gtagccaggt tgacgagctg catatcatta tgggttttga cgatacccgc gaccgcgcgt
    22921 tgtttgaaga cagcgccatg tcgcagcagc ccaccgtgcc ggatcgtctg cgctggttat
    22981 tgcaaacctt taaatatcag aaaaatatcc gtattcatgc tttcaacgaa gagggcatgg
    23041 agccatatcc gcacggctgg gatgtgtgga gcaacggcat caaaaagttt atggctgaaa
    23101 aggggatcca gccggacctg atctacacct cggaagaagc cgatgcgcca cagtatatgg
    23161 aacatctggg gatcgagacg gtgctggtcg atccgaagcg tacctttatg agtatcagcg
    23221 gtgcgcagat ccgcgaaaac ccgttccgct actgggaata tattcccacc gaagtgaagc
    23281 cgttctttgt gcgtaccgtg gcgatccttg gcggcgagtc gagcggtaaa tccaccctgg
    23341 taaacaaact tgccaatatc ttcaatacca ccagtgcgtg ggaatatggt cgcgattatg
    23401 tcttttcaca cctcggcggt gatgagatcg cattgcagta ttctgactac gataaaatcg
    23461 cgctgggaca cgctcaatat attgattttg cagtgaaata tgccaataaa gtggcgttta
    23521 ttgacactga ttttgtcact acccaggcgt tctgcaaaaa gtacgaaggg cgggagcatc
    23581 cgttcgtgca ggcgctgatc gatgaatacc gttttgatct ggttatcttg ctggagaaca
    23641 acaccccgtg ggtggcggat ggtttacgca gcctcggtag ttcggtggat cgcaaagagt
    23701 tccagaactt gctggtggag atgctggaag agaacaatat cgaattcgtg cgggttgaag
    23761 aggacgatta tgacagccgt ttcctgcgct gcgtggagct agtgcgggag atgatggggg
    23821 agcagagata accgtgacga aacggctcaa aggcgaggta taaaataagt tttttgatga
    23881 gttgaaaacc tctctggaag agactgtcga gattagaaag aacctgtacg ggtgacccgc
    23941 tacgaaattg cggatgctaa ggctattcgt gagcaacttc atactccaac cgaccggttg
    24001 cgtagccaga atgcaatcaa atctgacagt ctgcttcggc gcgcgctttg gtgtatttac
    24061 cggagtaaat cagcgtgccg tggaaaatta tgcgcagcgc ttctgctgat ttgcgtacct
    24121 tcgctgatga taagtcctgc actggcgcgc tgcgcgtaat agtccgccat tatgtcagtc
    24181 gcaacgtcac ccgcgcggga acgcgtcatc ggtggcatta caatacggtt tattaatggc
    24241 aacgcattca gcgcatattt agcaaattac gtaatgggtc cctcgcgcga ttatttatac
    24301 agttcggcac tcatggtgac ggtattgccc atgcgagcgc tggtaatttt atattcagag
    24361 gcgttcatct ctttcgcttt ctgcgcaata actgcttccg cagaatccag tgttgtaccg
    24421 gttgctgtca cgctttgcgc cgatgcgtta aacgccgcga cagagaaaag agaagctgta
    24481 aaaattgcga tgatcgtttt catgtttata ttccagagtg tcttaattgt ttggcgttta
    24541 ttaacgcgat ggggagagaa tacgcctgct gcctgataac ataaatgata caaatgacaa
    24601 tagactattc tgattttgtt aataatcaga cgccggacgt tccgacgcct gtcgatcgca
    24661 tcaggcaaca aagttgtcct tcagcgcctt gagaccatcc cgatagatgc cggtaaacag
    24721 cgcttcggcc tcttcgttcg tcacattcac cggggtgaat tcaccgaacc attccacacg
    24781 ggaaacctgg ctgtcggcgg tttcatgaac ggtaatggtg gagaggtaat ccaccaccgg
    24841 gaacggtgcc tgaataatgg aataggtata actgcgttga cggttatcaa acgcttccag
    24901 acgttcaacc acagtgccgc cgtcagaggt tgtcagcgaa cgaacgcgac cgccttctgt
    24961 cactacgctt ttcgggatga acggcagcca gtctggcagc gcatcaaagc cgcccatcaa
    25021 ttgccatacg gcgtcggcag aagccggaat ttcgatcgat acagttgttt tagccatgat
    25081 aattttctct taatttatag ggttacatag tcagggtgtc gacgacaccg ccatcgacgc
    25141 gcagggctgc ccctgtggtt gccgaggaaa gcggtgacgc gagataaacc gccagattcg
    25201 ctacttcttc cacatccgcc gcacgctgga tgatggagct cggacgcgag gttttaacaa
    25261 actcatcggc ctgttcacgc gcactgcgtc ccgattgtgc agcggcatcc gccagcatct
    25321 ctttcaggcc atcggtaaag gttggccctg gcagaatggc gttgacggtc acaccggtcc
    25381 ccgccagtcg tttcgccaga ccatgagaga ccgccagatt cgcagctttg gtgacgccgt
    25441 agttgatcat gtcgccagga attgccacgc cggattcgga cgagacaaag ataatccgcc
    25501 cccagccctg ttccgtcatg cctggcgcat agtgacgcgc cagacgaacg ccggagagga
    25561 cgttgacgtt atagaagtgc atccactcat catccggaac gctaaagaaa tctttatcgt
    25621 tgaaaatgcc cagattattg accagcacat cagccgttgg gactgcagcg atcagcgcag
    25681 ccacgccgtc ttcggtgctg agatcggcca ccacgccacg cagtgacgcc gtgggagttt
    25741 gtgtcgcaat cgctgccatg gcgtcatcaa cgccgcgctg tttacggccg acgacaacca
    25801 ccgttgcgcc tgcttttgcc agtccggcgg cgataccctg cccaatgccg gaggttgagc
    25861 cgctgaccac cgcgacttta cctgtcagat ccagtttcat cgttttctca ccaggttgat
    25921 taacgggtcg gcagcggagc gttttccgca accagatttt gacggcgcat ttctgcccag
    25981 aattcagccg gaacaggcgc attcagtgca gccagatctt ctgccatacg gccaggacgg
    26041 ctggagccag gaataaccgc tgcaacggcc gggtttgcca gcgcgaactg cagcgcagct
    26101 gctttaacgt tcacattgaa acgtgtggca atggcgttaa tttgcgccac tttctgctgg
    26161 atggccgcag aggctttctg atattcaaag tgctcgccgc ctgccagtac accagagctg
    26221 tacggaccgc ccacaacaat atccacgttc tgttccagtg cttctggcat caggcgttgc
    26281 agcgcacgtt cgtgatccag cagggaataa cgccccgcca gcaggaacgc atccggcttc
    26341 ggttcgtcca gcgccagcgt cagttcgcaa ggttcgacgc ggttcacacc cagtccccag
    26401 gctttaatca caccttcgtc acgcaaacgt gtcagtgcac gaaacgcacc ggtacgggca
    26461 atgttgaact gctccagcca gctgtcaccg tagaagtcct gcgccgggtc gtggatccag
    26521 acaatgtcca gacggtctgt tttcaggcgt ttcaggctgt cttcaatcga gcgcagcgtg
    26581 gcgtcagcag agtagtcatt cagaattttg tttttcaggc cgtgttcgaa caggccacct
    26641 ttttcaccga aatcccgttt tgccggatct tccatttcgt ccagcatgat gcgaccgact
    26701 ttgctactca gaacgtattc atcacgtgga tactgagaca gcgcttcgcc catgcggatt
    26761 tcagaaaggc cagagccgta cagcggcgcg gtatcaaagt aacgcacgcc caggttccag
    26821 gcttcgctca cggtggcgtg ggcctcttct tccggaatgg cacggaacat gttgccgagt
    26881 ggtgcgccgc ctaagccaat tttgccggga agtaaatttt tgatgctcat aagatgtcct
    26941 caattattcg gtttgggtta gggcgttttt ttgctatcgc cctgtgacgt ggatcacaat
    27001 accttgcgag gttgtgactt gataggacat aattcgtctg acttaagtcc tttgaggtct
    27061 aacatgattg atttacgcca actgaagtac ttcgtggtgg tcgcagaaga agagcacgtg
    27121 ggacgcgcag ccgagcgatt acatatctcg caatcaccgc tgagccgcca gattgcacaa
    27181 ctggaagaac ggctggggct gacgctgttt gagcgtaatc agcagcgcat tcgcctgacc
    27241 gctgatggac agacattcct tgccgaaaca cgttcgttgc tgacacacgc caaccgcctg
    27301 gagtcgttag gcaaacgcct gggacggggg gaagatgggg gcttgtgtat tggctatatc
    27361 gaaaacgcca tgcattccgg tgtactttct acggcgttac gtacgttacg tgtatcgcgt
    27421 ccgaatgtgc atatcgctct ttacagccaa ccaccggcga cccagattga aggcctgcgc
    27481 cagcgcagtc tcgatttttc tctggtcgat acgccgccca ccgacagcga gcaggatctg
    27541 gaatatattc aggttctgga tgatcgaatg ttgttggcct taccgcaaaa tcatcctctg
    27601 gcgcaggagg aaaaccttgt cgcgtcacaa cttggcgatg agcagtggat tgcggtgatt
    27661 cataacgacg atgcgcattc tcgcgatcat tttgtcgccg cctgtgttaa cgccgggttt
    27721 aatcctgata ttcgcctgga agcgggtgaa cccctcacgg cattaggttt ggtcgctgcg
    27781 ggattaggtc tggccttaat tcaacatagt ctgcgtcata atgcgccgga aggggtggta
    27841 ttacgtgaat tgccgtggat gaattattca acgcaattat gggcagcctg gcataaagcc
    27901 aaccaacgtc cgctggtttc ccatttccgc cagattttgc gcggtgaata aaacgttaag
    27961 cgggaaccat gagttcccgc ttaatttgag atgtcagtcc gcatctgagc taatacgctt
    28021 aaagaaaatt ctgccttttc cggggactaa tcatttaact aataccggat gcgatgctgg
    28081 cgcgtcgcat ccggcatttt acgcattact tcgcaatacg cttgtacttg atacgcttcg
    28141 gctccagcgc gtctgcgccc agcgtgcgtt tcttgtactc ttcgtactcg gtaaagttac
    28201 cttcgaagaa ctcaacttta ccttcatcct ggtagtccag gatgtgcgtg gcgatacggt
    28261 cgaggaacca acggtcgtgc gagataacca tcgcgcagcc cgggaactcc agcagggcgt
    28321 tttccagcgc acgcagggtt tcgatatcca ggtcgttggt cggttcgtcg agcagcagca
    28381 tgttgccgcc aacttgcagc agcttcgcca gatgcagacg accgcgctca ccgccggaga
    28441 gttcaccaac gcgtttaccc tgatcaaccc ctttaaagtt aaagcggcca acgtaggcac
    28501 ggcttggcat ctcggtgttg ccgatcttca tgatatccag cccgccggaa acttcttccc
    28561 aaacggtttt gctgttatcc attgagtcac ggaactgatc aaccgatgct agtttcaccg
    28621 tttcacctaa agtgatggtg ccgctgtccg gctgttcctg accggagatc atacggaaca
    28681 gggtcgattt acccgcaccg ttcggaccga tgatcccgac gatcgctcct ttcgggatcg
    28741 agaagctcag gtcatcaatc agcagacgat cgccatagga tttacgcagg ttgttgactt
    28801 ccagcacttt atcgccaaga cgcggtcccg gtggaataaa cagttcgttg gtttcgttac
    28861 gtttctgata ttcggtgctg ttcagctctt caaaacgcgc cagacgtgct ttacctttcg
    28921 actgacggcc tttagtcccc tgacgtaccc actccagctc tttctcaatc gacttacgac
    28981 gcgcagcttc ttgtgaagct tcctgcgcca gacgttgatc tttctgctcc agccaggagg
    29041 agtagttacc ttcccacgga ataccttcac cacggtccag ttcgaggatc cagcccgcca
    29101 cgttatcgag gaagtaacgg tcgtgggtaa tcgccacaac agtgccttcg aagtcgtgca
    29161 ggaagcgttc cagccacgcc acggattcgg catccaggtg gttcgtcggt tcgtcgagca
    29221 gcagcatgtc tggtttttcc agcagcaggc ggcacaacgc cacgcgacga cgctcaccac
    29281 cggagaggtt agcgattttc gcgtcccagt ccggcagacg cagcgcatcc gccgcacgct
    29341 ccagctgaac gttcaggttg tgaccgtcgt gagcctgaat gatctcttcc agacggcctt
    29401 gttcagcggc cagcttgtca aaatcggcat ccggatcggc gtacagcgca tacacttcat
    29461 ccaggcgttt cagggcgtta accacttcag aaaccgcttc ttcaatggac tcacgcacgg
    29521 tgtgttccgg gttcagctgc ggttcctgcg gcagataacc aatcttgata tccggttgcg
    29581 gacgcgcttc gccttcgatg tctttatcaa tgcccgccat aatgcgcagc agggtggact
    29641 tacccgcgcc gttcagaccc aggacaccaa tttttgcccc agggaagaaa ctcagagaga
    29701 tgtttttcaa aatatgacgt ttcggcggaa caactttgcc gacacgatgc atggtataaa
    29761 cgaattgagc cacgttggac ttcgcctcta tgtttatcgt gatgatgagt tttcaaaggc
    29821 gaagtgtagc ctttttcccc gcctaatccc agccggtcga tcaccctcgc agtaaaagta
    29881 aaaaagtgtc cgtaacgtgg cgtaaacggc aatgactggt tagcataaat ctattacgcg
    29941 gcatgacgct gcattgatgt atttacactt agaggatgcg cttgtggaaa aagccaaaca
    30001 agttacctgg cggctgttgg ctgccggtgt ctgtctgctg acggtcagca gcgtggcgcg
    30061 agccgactca ctggatgagc agcgtagccg ctacgcccaa atcaagcagg cctgggataa
    30121 tcgacaaatg gatgtggtcg aacaaatgat gcctggactg aaggattatc cgctttatcc
    30181 ctacctggaa taccgtcaga tcaccgatga cctgatgaat caaccggcgg tgacggtcac
    30241 taactttgtt cgcgctaacc ccacgcttcc tcccgctcgc acgctgcaat ctcgtttcgt
    30301 caatgaactg gcgcggcgtg aagactggcg tggcttgtta gcctttagcc cggaaaagcc
    30361 cggaactacc gaagcgcaat gtaattacta ctatgcgaaa tggaataccg ggcagagtga
    30421 agaagcctgg caaggggcga aagagctgtg gctaaccggc aagagccagc ctaacgcctg
    30481 tgacaagtta tttagcgtct ggcgtgcgtc aggtaaacaa gatccgctgg cgtatttaga
    30541 gcgtatccgt ttggcgatga aagcgggtaa cactggactg gtaacagtgc tggcagggca
    30601 gatgcctgcc gattaccaga ctatcgcctc ggcaatcatt tcactggcga acaaccctaa
    30661 tacggtactg accttcgcgc gtacaaccgg agcgaccgat tttacccgtc aaatggcggc
    30721 ggtggcgttt gccagtgtgg cgcggcagga tgctgagaat gcacggctga tgatcccatc
    30781 gcttgcccag gcgcagcagc ttaatgaaga tcagattcag gagctgcgcg atatcgtcgc
    30841 ctggcgtttg atgggcaacg atgtcaccga cgagcaggcg aaatggcgcg atgacgccat
    30901 tatgcgctcg caatccacgt cgttaattga acgccgtgtg cgaatggcgc ttggcaccgg
    30961 cgatcgtcgc ggcctgaata cctggctggc gcgtttgccg atggaggcga aagagaaaga
    31021 tgaatggcgt tactggcagg cggatttact gctggaacgc ggacgtgaag ctgaagcaaa
    31081 agagattttg catcaactca tgcaacagcg tggtttctac ccgatggttg cagcacaacg
    31141 catcggagaa gagtatgagc tgaagattga taaagcgccg cagaatgttg acagcgccct
    31201 gactcagggg ccggagatgg cgcgcgtgcg cgagttgatg tactggaatc tcgataacac
    31261 cgcgcgtagc gagtgggcca atctggtgaa gagcaagtca aaaacagagc aggcgcaact
    31321 ggctcggtat gctttcaata accaatggtg ggatcttagc gttcaggcaa cgatcgccgg
    31381 gaagctttgg gatcatctgg aagaacgatt cccgctggcc tacaacgatc ttttcaaacg
    31441 ttacaccagt gggaaggaga tcccgcaaag ctatgcgatg gcaattgccc gtcaggagag
    31501 tgcctggaat ccgaaagtga aatcgccggt aggggccagc ggcctgatgc agattatgcc
    31561 gggcacggcg acccatacgg tgaagatgtt ctctattcct ggttatagca gcccaggaca
    31621 attgctggat ccggaaacga acatcaacat tggcaccagt tacctgcaat atgtttatca
    31681 gcagtttggt aataaccgta ttttctcctc agcagcttat aacgccggac cggggcgggt
    31741 gcgaacctgg cttggcaaca gcgccgggcg tatcgacgca gtggcatttg tcgagagtat
    31801 tccgttctcc gagacgcgcg gttatgtgaa gaacgtgctg gcttatgacg cttactaccg
    31861 ctatttcatg ggggataaac cgacattgat gagcgccacg gaatggggac gtcgttactg
    31921 atccgcacgt ttatgatatg ctatcgtact ctttagcgag tacaaccggg ggaggcattt
    31981 tgcttccccc gctaacaatg gcgacatatt atggcccaac aatcacccta ttcagcagcg
    32041 atggcagaac agcgtcacca ggagtggtta cgttttgtcg acctgcttaa gaatgcctac
    32101 caaaacgatc tccatttacc gttgttaaac ctgatgctga cgccagatga gcgcgaagcg
    32161 ttggggactc gcgtgcgtat tgtcgaagag ttgttgcgcg gcgaaatgag ccagcgtgag
    32221 ttaaaaaatg aactcggcgc gggcatcgcg acgattacgc gtggatctaa cagcctgaaa
    32281 gccgcgcccg tcgagctgcg ccagtggctg gaagaggtgt tgctgaaaga tcactgatac
    32341 accgcattat gaaacggact cagcgccagg atcaccgcct ggtgatagac gctggcgcgg
    32401 gtaagtttcc cggcggtaaa cacgccgatc gccccttcct tacgaccaat ctcatcaata
    32461 ccggtatagc gcgacatcac gggaccgagc gcctcgcctt cacgcacttt ttccagaatc
    32521 accgcaggta acggcaaagt agccgaacgc gcctcgccgc gctggctggc gttttcaatc
    32581 accacccaac tgaaggtgct gtcgccatca atgccagctt caatcgccac ccaaaaatca
    32641 gcctctggat gtaaacggcg ggcatttgct acccgatttc gtgcgccagc gcgcgtttcc
    32701 tcactgccaa agggctgttc cggtacaccg ctctcgacgg caacggatgc aatatggcag
    32761 gatccttcgc cgaagatctc gtgaaatgcc tgcagaatgg cctgaatttt agcgggattg
    32821 gtggtcgcac agacaacttg gtgcataatc agcattactc agaaaattaa cgttacagca
    32881 gtatacggaa aaaaagcatg ttacaggtat acctagtccg ccacggtgaa acgcagtgga
    32941 acgccgagcg acgtattcag ggccagtctg acagcccgct gaccgccaaa ggtgagcaac
    33001 aggcgatgca ggtggcaacc cgtgccaaag agcttggcat tacgcatatc atcagtagcg
    33061 atttaggacg cacccggcgt acggcggaaa tcatcgccca ggcctgcggc tgtgacatca
    33121 tctttgattc tcgcctgcgt gaattaaaca tgggtgtgct ggaaaaaaga catatcgatt
    33181 ctctgaccga agaagaagag aactggcgtc ggcagctggt caatggcacc gttgacgggc
    33241 gtattcctga aggcgagtca atgcaggagc tcagcgatcg cgtcaatgcg gcactggaat
    33301 cttgccggga cttaccgcag ggaagccgac cgctgctggt gagccacggt attgcactgg
    33361 gatgcctggt gagtacgatt ctcggattac cagcatgggc agagcgtcgc ttacgtctgc
    33421 gtaactgttc tatttcgcgc gtggattatc aggaaagcct gtggctggcg tctggttggg
    33481 tcgttgaaac cgcaggggac atctcgcatc tggacgcccc tgcattagat gagctgcagc
    33541 gttaacgacg gatcggaatc agcagttcac agcgcagatt aattgggcga tctcccgctt
    33601 tggcatcttc tgccgggtag tatcgctcaa tatcctgacc tttacggcgc gtcaggttga
    33661 gcattggcat acacgttccg tataccgtca ggataaactc ctgcacgccg gttcccagac
    33721 cttcataggt aaacatcaca tattcgccgc cctgcagcat caccggatgc cccgtcagta
    33781 catagccatc tgcctgatcc tgggctaacg cggtggtata gaatacttct tgttcgtcgt
    33841 ctttatcctg actcggacgc gtttcattca ggccgtagag caccggcgga atggtcggcg
    33901 cgttgccgag aaaatcgtgc cagaactgat aacgcatttc atggcggaaa tcagagattt
    33961 gctccagcga acaggagtag ctctgggtaa caccaatcag cggcgtatct tccagggtga
    34021 caaatttgtg ctctggcata gtgaattcac ccaggcgtag cggcgggcga ataccaaagg
    34081 cgctccattc aggagaacgg cggtaaagtg caggagtctg ggcaaactgc ttcttgaatg
    34141 cgcgggtaaa tgtctgttga gagtcgaagc ggtattgcag cgcgatgtcc agaatcggac
    34201 gcgcagtcag gcgtagtgcg accgccgatt tcgacaaacg acgagcacga atatacgcgc
    34261 caatagcatg gccagtgaca tctttaaaca ttctctgtaa gtgccacttg gaataacctg
    34321 ctttcgccgc tacattgtcg agcgacaggg gctgatccag atgaccttcc agccagatta
    34381 aaaggtcgcg aataatgccg gcctgatcca taaaatatcc tcatcctttc aacaacgagc
    34441 acctgacatc aggtaattgg ataatagcat tttttgctgt tttagcattc agtgtttttt
    34501 tcttagtaga gtatgtttta ggacttgacg gaaataaaag tattgagatt ttgttcttaa
    34561 tcaatatgtt atttaccgtg acgaactaat tgctcgtgta atagataaaa atggtaacaa
    34621 tatgaaatac aagcatttga tcctgtcttt aagcctgata atgctggggc cattggctca
    34681 tgcagaagag attggttcgg tcgacaccgt atttaaaatg atcggcccgg atcacaaaat
    34741 tgttgtggaa gcctttgacg atcccgatgt gaaaaatgtc acctgttatg tgagccgggc
    34801 gaaaaccggt ggtattaaag ggggattggg cctggcagaa gatacctctg atgcggccat
    34861 ttcttgtcag caagtcgggc cgattgaact gtcggatcgt attaaaaacg gcaaagctca
    34921 gggcgaggta gtattcaaaa aacgcacgtc cctggtcttt aagtcgttac aggtcgtgcg
    34981 cttttatgat gccaaacgca acgcgctcgc ttatctggct tactccgaca aagttgtaga
    35041 aggttcgccg aaaaacgcga ttagcgcggt tcctgtcatg ccgtggcggc aataacagag
    35101 gcgatttatg caacgggaaa cggtctggtt agtggaagat gagcaaggga tagccgacac
    35161 gctggtctac atgttgcagc aggaaggttt tgccgtcgag gtctttgagc gaggcttgcc
    35221 ggtgctggat aaagctcgcc agcaggtacc cgacgtcatg attctcgatg ttggtctgcc
    35281 ggatattagc ggctttgaat tgtgccgcca gttactggcg ctccatccgg cgttacctgt
    35341 actgttcctg acggcccgaa gtgaagaggt cgatcgcctg cttgggctgg aaattggcgc
    35401 tgacgactat gtggctaaac cgttttcacc ccgcgaagtg tgcgccaggg tgcgcacctt
    35461 actgcgtcgg gtgaagaagt tctcgacgcc gtctcccgtc atccgtattg gacattttga
    35521 attgaatgaa cccgcggcgc agatcagctg gtttgacacg ccattaacgc tgacgcggta
    35581 tgagttttta ttgctgaaga cgttactcaa gtcgccaggc cgcgtctggt cccgccagca
    35641 actgatggat agcgtgtggg aagatgcgca ggacacctac gatcgcaccg tcgataccca
    35701 cattaaaacg ttgcgtgcca agctgcgcgc catcaacccc gatctttcac cgattaatac
    35761 tcatcgcggc atgggatata gcctgagggg cctgtaatgc gtatcggcat gcggttgctg
    35821 ctgggctatt ttttactggt ggcggtggca gcctggttcg tactggccat ttttgtcaaa
    35881 gaagttaaac cgggcgtgcg aagagcaacg gaggggacat tgatcgacac cgcaacgttg
    35941 ctggcggagc tggcgcgtcc cgatttgctc tctggggacc caacgcatgg gcaactggcg
    36001 caggcgttta atcagctaca gcatcgcccg tttcgcgcca atatcggtgg cattaacaaa
    36061 gtgcgcaatg aatatcatgt ctatatgacc gatgcgcagg gcaaagtatt gttcgattcg
    36121 gcaaataaag ccgttggaca ggattattcg cgctggaatg acgtctggct aacgttgcgt
    36181 ggtcagtatg gtgcgcgcag cacgttgcaa aatcctgccg atcccgaaag ttcggtgatg
    36241 tatgttgccg cgccgattat ggacggctcg cggcttattg gcgttttgag cgtaggcaaa
    36301 ccgaacgcgg cgatggctcc ggtcattaaa cgcagcgagc ggcgaatttt atgggccagc
    36361 gccattttgt tggggattgc actggtgatt ggcgcaggca tggtttggtg gatcaaccgc
    36421 tccattgccc ggctcactcg ctatgctgat tccgtcactg acaataagcc cgttcctctt
    36481 cccgatctcg gtagtagcga gctgcgtaaa ctcgcgcagg cgctggaaag tatgcgcgtg
    36541 aagctggaag ggaaaaacta tattgagcag tatgtttacg cattaactca tgagctaaaa
    36601 agcccactgg cggcgattcg tggagcggcg gaaattttac gcgaaggtcc gccgccggaa
    36661 gtggtggctc gttttactga caacattctg acgcaaaatg cgcgtatgca ggcattggta
    36721 gaaacgttac tacgccaggc aagactggag aatcgtcagg aagtcgttct gactgctgtt
    36781 gatgtggcgg cattattccg ccgcgtcagc gaagcgcgca ccgtgcagtt ggcagaaaaa
    36841 aaaatcactc tgcatgttac gcccaccgag gttaacgttg ctgctgaacc ggcgttactg
    36901 gagcaggcgc tgggaaattt actggataac gccatcgatt ttacccccga gagcggttgc
    36961 atcacgctaa gcgccgaagt ggatcaggaa cacgtcacgc ttaaggtgct ggataccggt
    37021 agtggtattc ctgactacgc gctttcacgt atttttgaac gcttttactc tttgcctcgt
    37081 gcaaatggtc aaaaaagcag cggtctgggg ttggcgttcg tcagtgaggt cgcccgtttg
    37141 tttaacggcg aagtcacgct gcgcaacgtg caggaaggtg gcgtgctggc ttcgcttcga
    37201 cttcaccgtc acttcacata gcttcaaatt cttcccacat agccttcgta tcctgctgcc
    37261 attgcaaagg agaagactat gttgaaatcc cccctgttct ggaaaatgac caccctgttt
    37321 ggtgcagtat tgctgttgtt gattccgata atgctgattc ggcaggtgat tgtcgaacgt
    37381 gctgattacc gtagcgatgt ggaagatgcg attcgccaaa gtaccagcgg gccgcaaaaa
    37441 ctcgttgggc cgctcatcgc tattcctgtg accgagcttt atacggtgca ggaagaggat
    37501 aaaaccgtgg agcggaaacg aagttttatc catttttggt tacctgagtc attgatggtt
    37561 gatggcaatc agaacgtgga agaacgcaag atagggattt ataccggtca ggtctggcac
    37621 agtgatttaa cgctaaaagc cgatttcgat gtttcgcgtc ttagcgaact caacgcgcca
    37681 aatatcacct taggcaagcc atttattgtg attagcgtcg gggatgcgcg tggtattggt
    37741 gtggtgaaag cgcctgaagt taacggaacg gcgctgacca ttgaaccggg caccggctta
    37801 gagcaaggcg ggcagggcgt gcatatccct ttacccgaag gggactggcg caagcagaac
    37861 ctgaagctga atatggcact gaatttaagc ggtaccggcg atctttctgt ggtgcctgcc
    37921 gggcgtaata gcgaaatgac cttaaccagc aactggccgc atcccagttt tttaggtgat
    37981 tttctaccag ccaaacggga agttagcgag tcaggttttc aggcgcaatg gcaaagcagc
    38041 tggtttgcta ataatctcgg tgagcgtttt gcttcaggca atgataccgg ctgggaaaac
    38101 ttccccgcgt ttagcgtcgc agtgacgacg ccagccgatc aataccaatt aacggaccgg
    38161 gcgactaagt acgccattct gctgattgca ctgactttta tggcgttctt tgtttttgaa
    38221 acgctcaccg cgcaacgttt acacccaatg caatatttgc tggtggggct ttcattggtg
    38281 atgttttatt tgctcttgct ggcgctttct gaacatatcg gttttaccgt ggcatggata
    38341 atagccagtc tgattggggc gttaatgaac ggtatttatt tgcaggcggt attgaaaggc
    38401 tggcgcaata gcatgttgtt taccctcgcg ctgttgttgc tggatggtgt gatgtgggga
    38461 ctgctcaact ctgccgatag cgcgctgttg ttgggaacca gtgtgctggt ggtggcgctg
    38521 gccggcatga tgtttgtgac ccgtaatatc gactggtatg cgttttcact gccgaaaatg
    38581 aaagccagta aagaagttac aacggacgat gaattacgta tctggaaata aggttgaaaa
    38641 ataaaaacgg cgctaaaaag cgccgttttt tttgacggtg gtaaagccga ttaatcttcc
    38701 agatcaccgc agaagcgata accttcaccg tgaatggtgg cgatgatttc cggcgtatcc
    38761 ggcgtagatt cgaaatgttt acgaatacgg cggatcgtca cgtctacagt acggtcgtgc
    38821 ggtttcagct cacggccggt cattttcttc agcagttcag cacgggactg aattttgcct
    38881 gggttttcac agaagtgaag catggcgcgg aactcgctgc gcggcagctt gtactgctcg
    38941 ccatcagggc cgatcaacga acggctgttg atgtccagtt cccaaccatt gaacttgtag
    39001 ctttcaacgc tacgacgttc ttcgctgaca gtacccagat tcatggtacg ggacagcagg
    39061 ttgcgtgcac gaatagtcag ttcacgcggg ttgaacggtt tggtgatgta gtcatctgca
    39121 ccgatttcga ggccgagaat tttatcgact tcgttgtcac ggccagtcag gaacatcaac
    39181 gcaacattcg cctgctcgcg cagttcacgc gctaacagaa gaccgttctt acccggcaga
    39241 ttgatatcca tgatcaccag gttgatgtca tattcagaga ggatctgatg catttccgcg
    39301 ccatctgtcg cttcgaaaac atcatagcct tccgcttcga aaatactttt caacgtgttg
    39361 cgtgttacca actcgtcttc aacgataaga atgtgcgggg tctgcatgtt tgctacctaa
    39421 attgccaact aaatcgaaac aggaagtaca aaagtccctg acctgcctga tgcatgctgc
    39481 aaattaacat gatcggcgta acatgactaa agtacgtaat tgcgttcttg atgcactttc
    39541 catcaacgtc aacaacatca ttagcttggt cgtgggtact ttccctcagg acccgacagt
    39601 gtcaaaaacg gctgtcatcc taaccatttt aacagcaaca taacaggcta agaggggccg
    39661 gacacccaat aaaactacgc ttcgttgaca tatatcaagt tcaattgtag cacgttaaca
    39721 gtttgatgaa atcatcgtat ctaaatgcta gctttcgtca cattatttta ataatccaac
    39781 tagttgcatc atacaactaa taaacgtggt gaa
//
LOCUS       DAOTRK010000022        39253 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.022, whole genome shotgun sequence.
ACCESSION   DAOTRK010000022 DAOTRK010000000
VERSION     DAOTRK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.022"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            20..931
                     /locus_tag="QIE41_001199"
     CDS             20..931
                     /locus_tag="QIE41_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530108.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTYSNNFYNNIQHAFNKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNETVNAMDKFLISFSLKDNGAEYIMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPERIKGYIRQ
                     AFGDVSLGYAVLDDIVSKLKRGKFDLQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITEEKICEKYKSTIEAFKKKQLALPV"
     gene            complement(1021..1575)
                     /locus_tag="QIE41_001200"
     CDS             complement(1021..1575)
                     /locus_tag="QIE41_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDS3530109.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMGLLQKLLK"
     gene            1900..2106
                     /locus_tag="QIE41_001201"
     CDS             1900..2106
                     /locus_tag="QIE41_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDS3530110.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2168..3511)
                     /locus_tag="QIE41_001202"
     CDS             complement(2168..3511)
                     /locus_tag="QIE41_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDS3530111.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3834..4472)
                     /gene="msrA"
                     /locus_tag="QIE41_001203"
     CDS             complement(3834..4472)
                     /gene="msrA"
                     /locus_tag="QIE41_001203"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDS3530112.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4678..6411
                     /gene="tamA"
                     /locus_tag="QIE41_001204"
     CDS             4678..6411
                     /gene="tamA"
                     /locus_tag="QIE41_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDS3530113.1"
                     /translation="MRYIRQLCCVSLLCLSGAAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6408..10187
                     /gene="tamB"
                     /locus_tag="QIE41_001205"
     CDS             6408..10187
                     /gene="tamB"
                     /locus_tag="QIE41_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDS3530114.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLDCLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWKIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQIQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10190..10531
                     /locus_tag="QIE41_001206"
     CDS             10190..10531
                     /locus_tag="QIE41_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDS3530115.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRIRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10743..10994
                     /gene="chpS"
                     /locus_tag="QIE41_001207"
     CDS             10743..10994
                     /gene="chpS"
                     /locus_tag="QIE41_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDS3530116.1"
                     /translation="MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGDEIW"
     gene            10988..11338
                     /gene="chpB"
                     /locus_tag="QIE41_001208"
     CDS             10988..11338
                     /gene="chpB"
                     /locus_tag="QIE41_001208"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDS3530117.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVWMMDLRARLAKRIGLAAGE
                     VVEEALLRLQAVVE"
     gene            complement(11542..12072)
                     /gene="ppa"
                     /locus_tag="QIE41_001209"
     CDS             complement(11542..12072)
                     /gene="ppa"
                     /locus_tag="QIE41_001209"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDS3530118.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12382..13338
                     /gene="ytfQ"
                     /locus_tag="QIE41_001210"
     CDS             12382..13338
                     /gene="ytfQ"
                     /locus_tag="QIE41_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDS3530119.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13470..14972
                     /locus_tag="QIE41_001211"
     CDS             13470..14972
                     /locus_tag="QIE41_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDS3530120.1"
                     /translation="MNTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIDREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTL
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAQRFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14986..16008
                     /locus_tag="QIE41_001212"
     CDS             14986..16008
                     /locus_tag="QIE41_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3530121.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            15995..16990
                     /gene="yjfF"
                     /locus_tag="QIE41_001213"
     CDS             15995..16990
                     /gene="yjfF"
                     /locus_tag="QIE41_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDS3530122.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(17023..18021)
                     /gene="fbp"
                     /locus_tag="QIE41_001214"
     CDS             complement(17023..18021)
                     /gene="fbp"
                     /locus_tag="QIE41_001214"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDS3530123.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            18197..19570
                     /gene="mpl"
                     /locus_tag="QIE41_001215"
     CDS             18197..19570
                     /gene="mpl"
                     /locus_tag="QIE41_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDS3530124.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDALADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19720..20271)
                     /locus_tag="QIE41_001216"
     CDS             complement(19720..20271)
                     /locus_tag="QIE41_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDS3530125.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20365..21717
                     /gene="pmbA"
                     /locus_tag="QIE41_001217"
     CDS             20365..21717
                     /gene="pmbA"
                     /locus_tag="QIE41_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDS3530126.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21900..22286
                     /gene="cybC"
                     /locus_tag="QIE41_001218"
     CDS             21900..22286
                     /gene="cybC"
                     /locus_tag="QIE41_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDS3530127.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            22478..22720
                     /locus_tag="QIE41_001219"
     CDS             22478..22720
                     /locus_tag="QIE41_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDS3530128.1"
                     /translation="MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRM
                     PVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL"
     gene            22710..23000
                     /locus_tag="QIE41_001220"
     CDS             22710..23000
                     /locus_tag="QIE41_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDS3530129.1"
                     /translation="MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKL
                     RDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGKRENSAVYEDATKRLDE"
     gene            complement(23001..23465)
                     /gene="nrdG"
                     /locus_tag="QIE41_001221"
     CDS             complement(23001..23465)
                     /gene="nrdG"
                     /locus_tag="QIE41_001221"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDS3530130.1"
                     /translation="MNFHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(23645..25783)
                     /gene="nrdD"
                     /locus_tag="QIE41_001222"
     CDS             complement(23645..25783)
                     /gene="nrdD"
                     /locus_tag="QIE41_001222"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDS3530131.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(26177..27832)
                     /gene="treC"
                     /locus_tag="QIE41_001223"
     CDS             complement(26177..27832)
                     /gene="treC"
                     /locus_tag="QIE41_001223"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDS3530132.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRVLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27882..29303)
                     /gene="treB"
                     /locus_tag="QIE41_001224"
     CDS             complement(27882..29303)
                     /gene="treB"
                     /locus_tag="QIE41_001224"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDS3530133.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(29422..30369)
                     /gene="treR"
                     /locus_tag="QIE41_001225"
     CDS             complement(29422..30369)
                     /gene="treR"
                     /locus_tag="QIE41_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDS3530134.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKVHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIETLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            30554..30607
                     /gene="mgtL"
                     /locus_tag="QIE41_001226"
     CDS             30554..30607
                     /gene="mgtL"
                     /locus_tag="QIE41_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDS3530135.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            30748..33444
                     /gene="mgtA"
                     /locus_tag="QIE41_001227"
     CDS             30748..33444
                     /gene="mgtA"
                     /locus_tag="QIE41_001227"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDS3530136.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIQIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLTGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(33650..34036)
                     /gene="ridA"
                     /locus_tag="QIE41_001228"
     CDS             complement(33650..34036)
                     /gene="ridA"
                     /locus_tag="QIE41_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDS3530137.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(34109..34570)
                     /gene="pyrI"
                     /locus_tag="QIE41_001229"
     CDS             complement(34109..34570)
                     /gene="pyrI"
                     /locus_tag="QIE41_001229"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDS3530138.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(34583..35518)
                     /gene="pyrB"
                     /locus_tag="QIE41_001230"
     CDS             complement(34583..35518)
                     /gene="pyrB"
                     /locus_tag="QIE41_001230"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDS3530139.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(35522..35656)
                     /gene="pyrL"
                     /locus_tag="QIE41_001231"
     CDS             complement(35522..35656)
                     /gene="pyrL"
                     /locus_tag="QIE41_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDS3530140.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35937..36332)
                     /locus_tag="QIE41_001232"
     CDS             complement(35937..36332)
                     /locus_tag="QIE41_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDS3530141.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPAQ"
     gene            complement(36463..37176)
                     /gene="bdcA"
                     /locus_tag="QIE41_001233"
     CDS             complement(36463..37176)
                     /gene="bdcA"
                     /locus_tag="QIE41_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS3530142.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            37247..37840
                     /locus_tag="QIE41_001234"
     CDS             37247..37840
                     /locus_tag="QIE41_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDS3530143.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLIKE"
     gene            37985..38437
                     /gene="tabA"
                     /locus_tag="QIE41_001235"
     CDS             37985..38437
                     /gene="tabA"
                     /locus_tag="QIE41_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDS3530144.1"
                     /translation="MIIGNIHNLQSWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            38560..>39253
                     /locus_tag="QIE41_001236"
     CDS             38560..>39253
                     /locus_tag="QIE41_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDS3530145.1"
                     /translation="MSKISGWNFSQHITSADNFKQKSEVINTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQSDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSHDLNNSQVSCKGSVDSTITDLLE
                     KSLNNALLAIRKE"
BASE COUNT         9596 a         9660 c        10481 g         9516 t
ORIGIN      
        1 aataaaataa ggtagccata tgttacccag gatcagacac aataatttta ttggtgcggt
       61 ggagttattc gtaaaatcat cgcatacaaa aacatattca aacaattttt ataacaatat
      121 ccagcatgca tttaataaaa aagattggat ttcaaattat gatagcctgt taactttgag
      181 ggagttcttt cgttgcgcaa ctcagattga taaaaatagt tatcaggtac tttcatcaaa
      241 aaatgaaact gttaacgcca tggataagtt tcttatcagt ttcagcctta aagataacgg
      301 tgccgaatat ataatgacat tgcgaggcag tggatttgaa tacgaagaaa ttcccataac
      361 aataaatgag tataactcat tcatggattt caaaaaccgc gagtttccgt tagaacaaaa
      421 cagaagattg tatgcctggg atattctgca gaaaaaacag tctgacatac cggaaaggat
      481 taaaggttat attcgccagg cgtttggtga cgtgtcttta ggttatgctg tattggatga
      541 tatagtaagt aaattaaaac ggggtaaatt tgacctccaa ataccaggag gtggaattaa
      601 agaatgcgat ggatggtata tttacgaaaa aataattgat gataattttg ccatagtcat
      661 agaatctttg ggctttgcgc ttaaaatcta tggaggggat gaacgttttc gtaatggttc
      721 ctctgttgtt ttggaggatg aagattactc tcttatttat aattttctgg ttaatgcagg
      781 ttgccaacaa gtcgaattag ccgaacaagt ggatgcgata gtaagtgcga atctggccgc
      841 agatagtgat attaccgaag aaaagatttg cgagaaatat aaatcgacca ttgaggcgtt
      901 taaaaaaaag caactagcgt taccagtatg agttcggcgt aagaactcag aggcttaatt
      961 cccctatccc atagataacg ataggggaaa attatttttg cagaatttat gttattgcca
     1021 ttatttgagc aacttttgca gcaggcccat cacttgctgt acctcttccg gtgtgagcgc
     1081 cccgtctttg gcccattgca cgcgaccgtc tttatccagc accactacag cggaactttc
     1141 ttcatccagc tgccaggcac cgcgcgcgac gccattgcta tcgacaataa actgcgacca
     1201 gggataaagc tttttattac tctccagact gctgcgcaca aacatgccgg aacccggaat
     1261 tgcgtcgtcg gtgttaacaa tggtggtggt ctggtaacga tcgtgcggta actttgctga
     1321 tttaatcgct tcaatcagcg tcgcgttttt ctcttttgca gaggtgcgac cagcaatatg
     1381 ttgcagtact cgcacttttc ccactaactg cgcgctgttc caggttttgt agctaaactg
     1441 atctttatca agcaccaact cgccccgatc ggtaatgcca atcggcggca ctcgctgacc
     1501 ggtttcgaac tgatgtgcgg aagccatcat cggcaacagc aggcaggtga gtgccagaat
     1561 cttgcgtagg gtcatggtgt ttccttctta tgatatgcag gtgatccgac cacttgggtc
     1621 tgagttttaa tcatatgtgc tatttatcga atttcccgca agtgtgatgc cagtttgcgg
     1681 tcaagcgcac aaatcatatg aaaaatgaat gcttatactg aagaccacgc ttcggtaaaa
     1741 agataattct gaataattgt aacctttagg taaaaaaagt tatacgcggt ggaaacattg
     1801 cccggatagt ctatagtcac taagcattaa aatttgcgcc tcataatagt tgggccgatt
     1861 gtggcaccgc acaggcgtaa tactcagcag gagataacaa tgaaaatttt ccaacggtac
     1921 aacccacttc aggtggcgaa gtacgtaaag atcctgttcc gtggacggtt atacatcaag
     1981 gacgttggcg cttttgaatt tgataagggt aagattctta tcccaaaagt gaaggataag
     2041 ctgcatttgt cagtgatgtc cgaagttaac cgtcaggtta tgcgtctgca aacagagatg
     2101 gcttaaccaa agtgctatgc agtaataaga cggctcctga ttcaggagcc gttgatgttt
     2161 ctgggggtta cgcgacgctt tcttctttat ctttagcgtc aggcagtttt ggcgaaaggg
     2221 cggtggcctt gctgtcgatt cgggtcacca gcagctggtc gatgcggtag ttatcgatat
     2281 ccaccacttc aaatttgtag ccggcgaatt tcaccgaatc ggtgcgtttc gggatcttac
     2341 gcagcataaa catcataaag ccgccgatgg tttcgtagtt gcccgactgc gggaactcgt
     2401 caatatccag cacgcgcatg acgtcgtcaa tcggagtgcc gccgtcaatc agccatgagt
     2461 tctcatcacg ggcgacaatc tgctcttcca gcccctgacc gaccagatcg cccatcagcg
     2521 tggtcatcac gtcgttgagg gtgatgatcc ccactaccag cgcgtactcg ttcatgatca
     2581 ccgcgaagtc ttcacctgcg gttttaaaac tttccaacgc ctctgaaagg gttaacgtat
     2641 ccggcacaat cagcgtgttg cggatttgta cgccgctgtt cagtgccagg ctttggttag
     2701 ccagcacgcg gttcagcagg tctttagaat cgacatagcc gatgatgtgg tcaatatctt
     2761 cattacagac gaggaactta gagtgcggat gttccgccac cttattcttc agactttgct
     2821 catcttcgtg gagatcaaac caaatcacgt tttcacgcgg tgtcattgac gacggaacgg
     2881 tacgggattc cagctcaaag acgttttcaa tcagctcgtg ttcctgttta cgtaacacgc
     2941 ccgccagcgc accagcttcc actaccgcgt agatgtcatc agaagtgatg tcatctttac
     3001 gtaccattgg cagtttgaaa atacggaaga tcatgttcgc cagtccgttg aagaaccaca
     3061 ccagcggggt gcaaacgtac aggcagaagc gcatcgggtt gatgatacgc aaagccacag
     3121 cttctggcgc aatcataccg atgcgtttcg gggttaaatc cgcaaacagg ataaacatgc
     3181 cagtcactaa cgagaaagag agaataaagc tcagttgctc agagagttct gccgacatat
     3241 agcgggagaa caggctgtga aaagctggag aaaatgccgc atcaccgacg ataccgccga
     3301 gaatcgccac tgcgttcaga ccgatttgga ccacggtaaa gaacatgccg ggattttcct
     3361 gcatattcag aacgcgttgg gcatttatat tgccttcatc agccagcagt ttaagtttga
     3421 ttttgcgtga agcggcaagc gagatctcgg acatcgagaa gaacgcactt acagcgatca
     3481 agcagagtat gactaaaata ctgtttaaca tatcttatcc gacctttcag gccagatcct
     3541 cggaagggga agttgattaa tttgtgtgaa atacacattg aaagccgatt cgaagaaagt
     3601 gaaccggcat tttcagtggc tagtatagcg taaggtactg taaagccgcc agagggttaa
     3661 aattcagact gctgataaat gaaaaactgc ctgatgcgct atgccgatca ggcctacgca
     3721 gtccccacaa tatattgatt ttggacgatt ttgtaggcct ggcaaggcgt ttacgccgca
     3781 tccggcatag acaaatagca ctttatcaac aatctacatt tgtacccgta acgctatgct
     3841 tccggcggca gacagacgcc aattccgcca attccacagt aaccatacgg gtttttatgc
     3901 agatattgct ggtggtcatc ttcggcataa taaaacggtg tggcgttcgc gatttccgtg
     3961 gtgatgtgac gatcgtcatc ggcggcaagc atcgccgcct gaaaacgttc cagactggcg
     4021 cgagcagcag catcctgttc tggggtcagt ggataaatcg ctgaacgata ctgcgtgccg
     4081 tggtcattgc cctgacgcat tccctgggcg ggatcgtgat tctcccaaaa tacttgtagc
     4141 aactgttcat agctgatgac ggaaggatcg taaacaatgc gtaccgcttc ggcatgaccc
     4201 gtatcaccgg agcacacttc ccgataagtc ggatttggcg tatagccgcc ggtatagcct
     4261 gcggcggtgc tgtaaacgcc gggtaactgc cagaacagac gctccacacc ccagaaacaa
     4321 cccatcgcaa aaatggcaat ctccattccg tcaggtacat tggtcattga gtgaccgttg
     4381 accgcatgca gtgtggctac gggcatcggg gtgttacgtc caggcagggc atcggcgggg
     4441 gaaaccagat gctttttatc aaataaactc atggtgtcgc tctcccgaaa atcggtcatt
     4501 ggggttaagg ttgtaacaag aggcgtattt gcacacaata accgctgtga ataggtctaa
     4561 agtaaaacat aagaaatatt tggggtttag tctgcttttt aatccatatt actggatttt
     4621 tgttaagccg tttaacggcg ttccaggggc aggaaaaaag gatattcagg agaaaatgtg
     4681 cgctatatcc gacagttatg ctgtgtaagc ttactctgct taagcggagc tgccgtcgcc
     4741 gcgaacgtcc gtctacaggt cgaggggtta tcgggacagc tggaaaagaa cgttcgtgcg
     4801 cagctttcta cgattgaaag tgatgaagtg acgccagacc gtcgctttcg cgcacgcgtc
     4861 gatgatgcca tccgcgaagg tctgaaagcg ctgggttatt accagccgac cattgaattt
     4921 gatctccgtc caccgccaaa gaaagggcgg caggtattga tcgccaaagt cacgccaggc
     4981 gtgccggtgt taattggcgg caccgatgtg gtattgcgcg gcggcgcgcg gaccgataaa
     5041 gactatttga aattgctcga tactcgcccg gctattggta cggtactgaa ccagggcgat
     5101 tatgaaaatt tcaaaaagtc cttaaccagc attgcgttgc gtaaaggtta tttcgatagc
     5161 gaatttacca aagcgcagct gggcattgcg ctcggcctgc ataaagcctt ctgggatatt
     5221 gattataaca gtggcgaacg ttaccgcttt gggcatgtga cctttgaagg atcacaaatc
     5281 cgcgatgaat acctgcaaaa tctggtgccg tttaaagagg gcgatgagta cgaatcgaaa
     5341 gatctggcgg aactgaaccg tcgactttct gctaccggct ggtttaattc ggtggtggtg
     5401 gcaccacaat ttgataaagc gcgcgaaacg aaagtattac cattgacggg cgtggtttcg
     5461 ccgcgaactg aaaacaccat cgaaaccggg gtcggttact ctacggacgt agggccacgt
     5521 gttaaggcaa cgtggaaaaa accatggatg aactcgtacg gtcatagcct gaccaccagt
     5581 accagtattt ccgcgccgga acagaccctc gacttcagct ataaaatgcc actgctgaag
     5641 aatccactgg aacaatatta tttggtgcag ggcggtttta agcgcactga cctgaacgat
     5701 accgagtctg actccactac gctggtggct tctcgctact gggatctttc cagcggctgg
     5761 cagcgtgcca ttaacctgcg ctggagtctc gaccacttta cccaaggtga aattaccaac
     5821 accacgatgc tgttttatcc tggggtgatg attagccgca cgcgttctcg cggtggcctg
     5881 atgccaacct ggggcgactc gcaacgctac tctatcgact actccaacac ggcctggggc
     5941 tcagatgtcg atttctccgt tttccaggcg cagaacgtct ggatccgcac actgtacgat
     6001 cgccatcgtt ttgttacacg tggcacgctg ggctggattg aaaccggtga tttcgacaaa
     6061 gtaccgccgg atctgcgttt cttcgccggg ggcgatcgca gtattcgcgg ctacaaatac
     6121 aaatcaatcg ctccgaaata cgccaacggt gacctgaaag gggcctcgaa gttgataacc
     6181 ggatcgctgg agtaccagta caacgtgacc ggaaaatggt ggggcgcggt gtttgtcgat
     6241 agcggcgaag cggtaagcga tattcgccgc agcgacttta aaaccggtac cggggtcggc
     6301 gtgcgctggg aatcgccggt cgggccaatc aaactcgatt ttgccgtacc ggtcgcggat
     6361 aaagacgaac acgggttaca gttttacatc ggtctggggc cagaattatg agtttatgga
     6421 aaaaaatcag cctcggcgtg gttatcgtta tcttactgtt gctgggatcg gtggcgtttc
     6481 tggtgggcac caccagcggc ctgcatctgg tatttaaagc ggcggatcgc tgggtgccag
     6541 gactggatat tggcaaagtc accggcggct ggcgcgatct caccttgtct gacgttcgtt
     6601 atgagcagcc aggcgtggcg gtaaaagcgg gcaatctgca tctggcggtt gggctggatt
     6661 gtctttggaa cagtagcgtt tgtattaatg acctggcgct gaaagacatt caggtcaaca
     6721 tcgacagtaa aaaaatgcct ccttctgaac aggttgaaga agaggaagat agcggcccgc
     6781 tggatctctc cacgccatat cccatcaccc tgacacgggt ggcgctggat aacgtcaaca
     6841 tcaagattga tgacaccacg gtatcggtga tggacttcac ctccggcctg aactggcagg
     6901 agaaaaccct gaccctgaaa ccgacgtcgc tgaaaggcct gttgattgct ttgccgaaag
     6961 tggcggaagt ggcgcaggaa gaagtggtcg aaccgaagat tgaaaatccg cagccggagg
     7021 aaaagccgct cggcgaaacg ctgaaagatc tcttttctcg cccggtattg ccggaaatga
     7081 ccgacgtgca tttgccgctt aacctgaaca ttgaagagtt taagggcgag cagctgcgcg
     7141 tgacgggcga cacagacatc accgtgcgca ccatgctgct gaaagtgagc agtatcgacg
     7201 gcaataccaa actggacgcg ctggatatcg actccaacca ggggattgtc aacgccagcg
     7261 gcacggcgca gctatcagac aactggccgg tggatatcac cctcaacagc acactgaacg
     7321 tggagccgtt gaaaggtgaa aaggtgaagc tgaaagtggg cggcgcgctg cgcgaacagc
     7381 tggagatagg cgttaacctt tctggtccgg tggatatgga tttacgcgcc cagacgcgac
     7441 tggcggaagc cggattgccg ctcaacgtgg aagtgaacag caaacagctt tactggccgt
     7501 tcactggaga gaagcagtat caggcggatg atctgaaact gaaacttacc ggcaaaatga
     7561 ccgattacac gctctctatg cgcacggcag tgaagggaca ggagatcccg ccagccacca
     7621 ttacccttga tgccaaaggt aatgaacagc aggtcaatct cgacaaactc accgtcgcgg
     7681 cgctggaagg gaaaactgaa ctcaaagcgt tgctcgactg gcagcaggcc attagttggc
     7741 gcggtgagct aacgcttaac ggcattaaca ccgccaaaga gttcccggag tggccgtcga
     7801 aactcaatgg cttgattaaa acccgcggta gcctgtacgg cggcacctgg cagatggagg
     7861 tgccagaact gaagctgacc ggtaacgtca aacagaacaa agtgaacgtt gacggcacgc
     7921 tgaaaggcaa cagttatatg cagtggaaga tcccagggct gcatctggaa ctggggccaa
     7981 acagtgccga agtgaaaggc gagctggggg taaaagatct caatcttgat gccaccatca
     8041 acgcgccggg gctggataac gcgctgccgg ggcttggcgg tacagcgaaa gggctggtga
     8101 aagtacgcgg cacggtggaa gcgccacaac tactggcaga tatcaccgcg cgcggcctgc
     8161 gctggcagga actttccgtg gcgcaggttc gcgtggaagg cgatatcaaa tccaccgatc
     8221 agattgcggg taaacttgac gtacgcgttg agcaaatctc gcagccggat gtaaatatca
     8281 acctcgtcac tctgaatgcc aaaggtagcg aaaaacagca cgagctacag ttgcggattc
     8341 agggcgaacc ggtttccgga cagcttaatc tggcaggaag ttttgatcgc aaagaagaac
     8401 gctggaaggg aacacttagc aatacccgct tccagacgcc ggtcggcccg tggtcgctga
     8461 cccgcgatat tgcgctggat tatcgcaaca aggagcaaaa aatcagcatc gggccacact
     8521 gctggcttaa cccgaatgcg gaactgtgcg tgccgcaaac cattgatgcc ggggctgaag
     8581 ggcgtgcggt ggtgaatctc aaccgcttcg acctggcgat gctgaaaccg tttatgccag
     8641 aaaccactca ggccagcggt atcttcacgg gtaaagcgga tgtcgcctgg gacaccacga
     8701 aagaggggct gccgcagggc agtatcaccc tttcggggcg taacgtgcag gtaacgcaaa
     8761 ccgtcaacga tgcggcgctg cccgtggcgt ttcagacgct gaatctgacg gcggagttgc
     8821 gtaacaaccg tgccgaattg ggctggacca tccgcctgac caataacggc cagtttgatg
     8881 gacaggtgca ggtgaccgat ccgcaaggcc gccgtaatct tggtggcaac gtcaatatcc
     8941 gtaacttcaa ccttgcgatg ataaacccta tctttacccg tggggaaaaa gctgcgggga
     9001 tggtgagtgc caacttacgt ctgggtggtg atgtgcaaag cccccagttg tttgggcaga
     9061 tacaggttac gggtgtggat atcgacggca actttatgcc gtttgatatg cagccgagcc
     9121 agcttgcggt caactttaac ggtatgcgct cgacgcttgc cggtaccgtg cggacccagc
     9181 agggtgagat ctacctgaac ggcgacgccg actggagcca gattgaaaac tggcgggcgc
     9241 gggtaacagc gaagggcagt aaagtgcgga tcaccgtgcc gccgatggta cgaatggatg
     9301 tatcgccaga tgttgtattc gaggctacac caaacctgtt taccctcgat ggtcgcgtgg
     9361 atgtcccgtg ggcgcgcatc gtggtgcacg atctgccgga aagtgcggtg ggcgtctcca
     9421 gcgatgtggt gatgcttaac gataacctgc aaccggaaga ggcaaaaacg gcgtcgattc
     9481 cgattaacag caacctgatt gtccacgtcg gcaacaatgt gcgcattgac gcctttggcc
     9541 tgaaagcgcg gctgacgggc gatcttaacg tcgtacagga taaacaaggg ctgggcctga
     9601 acgggcagat caacatccct gaagggcgtt tccatgccta tggtcaggat ctgattgtgc
     9661 gtaagggtga gttactgttc tctggtccgc cggatcaacc gtatcttaat atcgaagcta
     9721 ttcgtaaccc ggatgctaca gaagacgacg taatcgccgg agttcgcgtc actggtctgg
     9781 cggacgaacc gaaagcggag atcttctctg acccggccat gtcgcaacaa gctgcattgt
     9841 cttatttgct acgtggacaa gggctggaga gcgatcagag cgacagtgcg gcgatgacct
     9901 caatgctgat tggtctgggg gttgcacaaa gtggtcagat tgtgggtaaa atcggcgaga
     9961 cgtttggcgt aagcaattta gcgctcgaca cccagggagt aggcgactcc tcccaggtgg
    10021 tggtcagcgg ctatgtattg ccaggtctgc aagtgaaata cggcgtgggt atatttgact
    10081 ctatagcaac actcacgtta cgttatcgcc tgatgcctaa gctatatctg gaagccgtgt
    10141 ctggtgtaga ccaggcactg gatttgctct atcagttcga gttttagcaa tgcgaatatt
    10201 tgtctacggc agtttacgcc acaaacaagg caacagtcac tggatgacca atgcccagtt
    10261 actgggcgat ttcagtatcg ataactacca gttgtatagc ctgggccact atccaggcgc
    10321 agttccgggg aacggaacag tacacggtga agtttatcgt attgacaacg ccacgctggc
    10381 cgaacttgat gccttgcgca tcaggggcgg tgaatacgcg cgccagttga ttcagacgcc
    10441 gtacgggagt gcatggatgt acgtttatca acgacccgtg gatggattaa agctaattga
    10501 aagcggcgac tggttagaca gggataagta accatacgca tacgccacct tcgggtggcg
    10561 ttgttttttg cgaaacgact cgcattctgt tttgtaattc cctcaccttt tgcttttctc
    10621 tccgagccgc tttccatatc tgttaacgca taaaaaactc tgctggcgtt cacaaatgcg
    10681 caggggtaaa acgtttcctg tagcaccgtg agttatactt tgtataactt aaggaggtgc
    10741 agatgcgtat taccataaaa agatggggga acagttcagg tatggtcatt cctaatgttg
    10801 tgatgaaaga acttaactta cggccggggc agagcgtgga ggcgcaagtg agcaacaatc
    10861 aactgattct gacacccatc tccaggcgct actcgcttga tgaactgctg gcacagtgtg
    10921 acatgaacgc cacggaactt agcgagcagg atgtctgggg taaatccacc cctgcgggtg
    10981 acgaaatatg gtaaagaaaa gtgaatttga acggggagac attgtgctgg ttggctttga
    11041 tccagcaagc ggccatgaac agcaaggtgc tggtcgacct gcgcttgtgc tctccgttca
    11101 agcctttaat caactgggaa tgacgctggt ggcccccatt acgcagggcg gaaattttgc
    11161 ccgttatgcc ggatttagcg ttcctttaca ttgcgaagaa ggcgatgtgc acggcgtggt
    11221 gctggtgaat caggtgtgga tgatggatct acgcgcccgg ctggcaaagc gtattggtct
    11281 ggctgcgggt gaggtggtgg aagaggcgtt attacgtttg caggcggtgg tggaataagg
    11341 tgtgtttatt tatcgcgggc ataaaaaacc cctactaacc gaagcccggc gttcagggtt
    11401 attgcgctat actcgtcata cttcaagttg catgtgctgc gactgcgttc attcacccca
    11461 gtcacttact tctgtaagct cctggggatt cactcacttg tcgccttcct gcaagttgaa
    11521 ttatttagag tatataagaa cttatttatt ctttgcgcgc tcgaaggagg caacgatttc
    11581 agctttagcg gcttctgcgt tttcccagcc ttcaactttc acccacttgc ctttttcgag
    11641 gtctttgtag tgctcgaaga agtgagcgat ctgtgctttc agcagttcag gcagatcgtt
    11701 aacgtcttta atgtgatcgt attctttgct cagcttgctg tgcggaaccg caaccagttt
    11761 cgcatcttca ccggcttcgt cggtcatttt cagaacgcca accggacggc aacggatcac
    11821 agaacccggc tgcagcgggt acggagtcgg gaccagtacg tcaaccggat caccgtccag
    11881 agacagggtg tggttgatgt aaccgtagtt gcacggatag aacatcgcgg tggacatgaa
    11941 gcggtcaacg aacagtgcgc cgctctcttt gtcgatttcg tatttgatcg gatctgcgtt
    12001 agccgggatc tcaataacaa cgtagatgtc ttccggcaga tctttacccg cagggacgtt
    12061 gagtaagctc atgtctgttt cctttaaaaa tatgtggcaa acaagtgccg agtattatag
    12121 ccaactcgcg ccgaatgtct tcgcttgttt tcttcttcat tttccttttt agccagtttt
    12181 aagatggttt aaagagcagg aaaatccata accgtagccg catttttcat agtgagatga
    12241 aagcgattac aaacttgtga ttaacgtttt atttactttt ttgaagtgtg atgtaacgca
    12301 atccgttaca taacgaattg tctatagttt tttcgcgaac atcttttcac caataataat
    12361 taccccgacg aggacaaccc tatgtggaaa cgcttacttg tagtctctgc agtctcggca
    12421 gccatgtcgt ctatggcgtt ggctgctcca ttaaccgttg gattttcgca ggtcggatcg
    12481 gaatccggct ggcgcgccgc agaaaccaac gtggcgaaaa gtgaagccga aaagcgcgga
    12541 attacgttga aaattgccga tggtcagcaa aagcaggaaa accagattaa agcggtacgt
    12601 tccttcgtcg cgcaaggggt ggatgcgatc tttatcgctc cagtggtagc gaccggttgg
    12661 gagccggtat taaaagaggc gaaagatgcc gaaatcccgg tcttcttgct tgaccgttcc
    12721 atcgatgtga aagacaaatc tctctatatg accactgtca ccgccgacaa catcctcgaa
    12781 ggcaagttga ttggtgactg gctggtaaaa gaagtgaatg gcaaaccatg caacgtggtg
    12841 gagctgcagg gcaccgttgg ggccagcgtc gccattgacc gtaagaaagg ctttgccgaa
    12901 gccattaaga atgcgccaaa tatcaaaatt atccgctcgc agtcaggtga cttcacccgc
    12961 agcaaaggca aagaagtgat ggagagcttt atcaaagcag aaaacaacgg caaaaacatc
    13021 tgcatggttt acgcccataa cgatgacatg gtgattggcg caattcaggc aattaaagaa
    13081 gcgggcctga agccgggtaa agatatcctc acgggttcca ttgatggcgt accggatatc
    13141 tacaaagcga tgattgatgg cgaagcgaac gccagtgttg aactgacgcc gaacatggca
    13201 ggtcctgctt tcgacgcgct ggagaaatac aaaaaagacg gcaccatgcc tgaaaagctg
    13261 accctgacca agtccaccct ttacctgcct gataccgcaa aagaagagtt agagaagaag
    13321 aaaaacatgg ggtattgagg ggcatcaatg cctaatgccg acttgtgggc cgccggataa
    13381 ggattcacgc cgcatccggc ggttgtgcga gatgcctgat gcgacgctgt tgtgtcttat
    13441 ctggcgcacg ctttacaacg ggggaaacta tgaatactaa ccaacaccag gaaatcctcc
    13501 gcaccgaagg attaagtaaa tttttccccg gcgtcaaagc gttagacaac gttgatttca
    13561 gcctgcgccg tggtgaaatc atggcgctgc tcggtgaaaa cggggcggga aaatcaacgc
    13621 taatcaaagc cttaaccggt gtctaccacg ccgatcgtgg caccatctgg ctggaaggcc
    13681 agactatctc accgaaaaat accgcccacg cacaacaact cggcatcggc accgtctatc
    13741 aggaagtcaa cctgctaccc aatatgtcgg tcgctgataa tctattcata gatcgcgaac
    13801 ccaaacgttt cggccttcta cgccgtaaag agatggaaaa gcgcgccacc gaactgatgg
    13861 catcttacgg tttctccctc gacgtgcgcg aaccgctcaa ccgcttttca gtggcgatgc
    13921 agcaaatcgt cgccatttgc cgggctatcg atctctctgc caaagtgctg atcctcgatg
    13981 aacccactgc cagtctcgac actcaggaag tagagttact gtttggcctg atgcgtcagt
    14041 tgcgcgatcg cggcgtcagc ctgatcttcg ttactcactt tctcgatcag gtctatcagg
    14101 tcagcgatcg gatcaccgtc ttacgcaacg gcagtttcgt aggctgtcgg gaaacccgcg
    14161 agctaccgca gatcgaactg gtaaaaatga tgctggggcg cgagctggac acccacgcgc
    14221 tacagcgtgc cggacgaaca ttgctgagcg acaaacccgt tgccgcgttc aaaaattacg
    14281 gcaaaaaagg aacgctcgca ccgtttgatc tcgaagtacg ccccggcgag atcgttggcc
    14341 tggcaggctt gctgggatca ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac
    14401 cagctgacag cggcacggcg ttgatcaaag gcaaacagca aaccctgcga tcgccacatc
    14461 aggcttcggt actgggtatc ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg
    14521 ccgccgcctc ggtgcgggaa aatatcatcc tcgctctcca ggcccagcgc ggctggctac
    14581 gaccgatttc ccgcaaagaa cagcaagaga ttgcccaacg ctttatccgc cagcttggca
    14641 ttcgcacacc ttcaactgaa caaccgattg aatttctctc cggcggcaat cagcaaaaag
    14701 tgttgctttc acgttggcta ctgacccgac cgcaatttct gatcctcgac gagccaacgc
    14761 gcggaattga tgttggtgcc cacgccgaga tcatccgcct gattgaaacg ctgtgcgccg
    14821 atggactggc gctgctggtg atctcctccg agctggaaga gctggtgggc tatgccgacc
    14881 gggtgattat catgcgcgat cgcaaacagg tagcggagat cccgctggca gagctttccg
    14941 ttccggcgat catgaacgcc attgcggcgt aaggagaaca gtgtgatgcc tcaatctctc
    15001 ccggacacta cgccgccgaa aaggcgcttt cgctggccaa caggaatgcc gcagctggta
    15061 gcactattgc tggtgctgct ggtcgacagc ctggtagccc cgcatttctg gcaggtggtg
    15121 cttcaggatg ggcgtttgtt cggtagccct atcgacattc ttaaccgtgc ggcccccgtt
    15181 gcgctactgg cgattggcat gacgctggtt atcgccactg gtgggattga tctctccgta
    15241 ggggcggtga tggctatcgc cggagccaca acggctgcga tgacggtcgc gggattcagc
    15301 ctgccgattg ttttgttaag cgccctgggc actggcatcc tggcgggatt gtggaacggc
    15361 atactggtag cgatcctcaa aattcagccg tttgttgcca ccctgatcct gatggtcgcc
    15421 gggcgcggcg tggcgcaact gatcacctcc ggacagatcg tcacgtttaa ctcgccggat
    15481 ctctcatggt ttggcagcgg atcgctattg ttcctgccaa cgccggtcat tatcgcggtg
    15541 ctgacgcttc tcctgttctg gctgttgacc cgcaaaacgg cactggggat gtttatcgaa
    15601 gccgttggta tcaatattcg ggcggcaaaa aatgccgggg taaacacgcg aatcatcgtc
    15661 atgctcacct acgtgttgag cgggctatgt gcggcgattg cgggcattat cgtggcggcg
    15721 gatattcgcg gtgccgatgc caataacgcc gggttatggc tggaactgga cgccattctc
    15781 gcggtagtga ttggcggcgg atcgctgatg ggcgggcgct ttaacctgct gctttcggtg
    15841 gtgggggcac tgattattca gggaatgaac accggaattt tgctttctgg atttccgccg
    15901 gagatgaacc aggtcgtaaa agcggtggtg gtgctttgcg tgctgattgt tcagtcgcaa
    15961 cgctttatca gtctgattaa aggggtacgt aaccgtgata aaacgtaatt tgccactgat
    16021 gatcaccatc ggcgtctttg tgttgggtta tatctactgt ctgacccagt ttcccggttt
    16081 tgcttccaca agagtgatct gcaatatcct gaccgataac gcttttctgg ggatcatcgc
    16141 cgttggcatg acctttgtga tcctctccgg tgggatcgat ctctccgtcg gttcggtgat
    16201 cgcctttact ggcgtgtttc tggcaaaagt gattggcgac ttcggcctct cgccgttgct
    16261 ggcatttccg ctggtgctgg taatgggctg tgcctttggc gcatttatgg ggcttttgat
    16321 cgacgccctg aagatcccgg catttatcat taccctggcg ggaatgttct ttttgcgtgg
    16381 cgtcagctat ctcgtttcgg aagagtcgat cccgataaac catcctgttt atgacacgct
    16441 ctcaagcctt gcgtggaaaa tccctggcgg cggtcgctta agtgcgatgg ggctgctgat
    16501 gctggcggtg gtggttatcg gcatattcct cgcgcatcgt acgcgttttg gtaatcaggt
    16561 gtacgccatt ggcggcaacg caacgtcggc gaacctgatg gggatttcca ctcgcagcac
    16621 cactattcgc atttatatgc tctctaccgg actggcaacg ctggcgggga ttgtcttctc
    16681 gatttatacc caggccggat atgcgctggc gggcgtaggt gtggaactgg acgctatcgc
    16741 ctcagtggtg attggcggta cgcttttgag cggtggcgtt ggaacggtat tagggacgct
    16801 ttttggcgtg gcgattcagg gactgattca gacttacata aactttgatg gcacgctgag
    16861 ctcatggtgg acgaaaatcg ccatcggtat tttgttgttt atttttatag cattacagcg
    16921 tggattaacg gtgctgtggg agaatcgtca gagttcgcct gtgacaagag tcaacattgc
    16981 gcagcaataa aaacgcctct ccgtgtggag aggcgcagga gattacgcgt ccgggaactc
    17041 acggataaag cgttcgacat cttcaaccat atggtcgttg ccgacaaaga acgaacggcg
    17101 ctggtgcagg gtttccggga tgatatccag aatacgctct ttgccatcgc tcgctttacc
    17161 gcccgcttgt tccgccagga atgccatcgg gttgcactca tacagtaaac gcagtttacc
    17221 gtccgggtgg ctggcggtgc ttgggtagag ataaataccg cctttcagca ggttacggtg
    17281 gaaatccgcg accagtgaac cgatataacg tgaggtataa gggcggttgg tggacttatc
    17341 ttcttcctgg cagaatttaa tgtacttctt caccccgttc ggaaacttaa tgtagtttcc
    17401 ttcgttgatg gagtaggttt tgcctttctc cgggaagcgc atccgttcct ggcacaggca
    17461 gaaaacgccg agcgaaggat cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac
    17521 cagcatggta gaggagccgt ataccacgta acctgccgca acctgtttgt taccaggctg
    17581 gaggaaatct tcttccgtta ccggcgtgcc gacaggcgta acgcggcggt agatggagaa
    17641 aatggtaccg acagagacgt taacatcgat gttggacgag ccatccaggg ggtccatcag
    17701 caccacgtat tttgcgtgtt cacagccttc aaagacgaca atctcatctt cttcttcaga
    17761 ggcaatgcct gcaacgatat cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa
    17821 caagtcgagt ttctgctgaa cctcgccctg cacattctca gcaccgctgg cacccaggat
    17881 atcaaccagt cctgctttgt tgatatcgcg atggataatc ttggcgccca gttttattgc
    17941 cgacagcaaa gcagtgagct caccggtagc atgagaaaac tcgtgctgct tttcgacaat
    18001 aaattcacct aacgttttca taaaactttc cctgcaatgt ttatggagta aagcgaccgc
    18061 aacaatctta acaaataatc tcaatgttgc gctcaggtga atcgcgccag caaattacgg
    18121 attatcctga aatgcgtttc tcacttgccc gacatatgcg taaaatgagc ggcagattaa
    18181 aaaaggatag tgacgtatgc gcattcatat tttaggaatt tgtggcacgt ttatgggcgg
    18241 tctggcgatg ctggcgcgcc agttaggcca tgaagtaacg ggttcggacg ccaatgtgta
    18301 tccgccgatg agcaccttac ttgagaagca aggcattgaa ctgattcagg gttacgatgc
    18361 cagccagctc gaaccgcagc cggatctggt gattattggc aacgccatga cccgtggaaa
    18421 tccgtgtgtg gaagcggtac tggaaaaaaa catcccttat atgtcaggtc cacagtggct
    18481 gcacgatttt gtgctgcgcg accgctgggt gctggccgtt gccggtacac acggcaaaac
    18541 taccaccgcg ggaatggcga cctggattct ggaacagtgc ggttacaaac cgggatttgt
    18601 gatcggcggt gtgccgggga actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt
    18661 tgttatcgaa gcggatgagt atgactgcgc cttcttcgac aaacgctcta aatttgtcca
    18721 ttactgcccg cgtacgctga tcctcaacaa ccttgagttc gatcacgccg atatctttga
    18781 cgacctgaaa gcgatccaga aacagttcca ccatctggtg cgtatcgttc cggggcaggg
    18841 ccgtattatc tggccggaaa acgacatcaa cctgaaacag accatggcga tgggctgctg
    18901 gagcgagcag gagctggtgg gcgagcaggg tcactggcag gcgaaaaagc tgaccaccga
    18961 tgcttccgaa tgggaagtct tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct
    19021 ggtaggcgaa cataatatgc acaatggcct gatggcgatt gcggcggctc gccatgttgg
    19081 tgtagcgccg gcagatgccg ctaacgcgct gggttcgttt attaatgctc gtcgccgtct
    19141 ggagttgcgt ggtgaagcga atggcgtcac ggtatatgac gattttgccc atcacccgac
    19201 ggcgattctg gcaacgcttg cggcgctgcg tggcaaagtt ggtggtacgg cgcgcattat
    19261 tgctgtgctg gaaccgcgct cgaataccat gaaaatgggg atctgcaaag acgatctggc
    19321 accttcatta ggtcgtgccg atgaagtctt cctgctgcaa ccggcgcata ttccgtggca
    19381 ggtggcagaa gtggcagaag cctgcgttca gcctgcacac tggagcggcg atgtcgatgc
    19441 gctggcagat atggtggtga aaaccgctca gcctggcgac catattctgg tgatgagcaa
    19501 tggcggtttt ggtgggatcc atcagaaact gctggatggg ctggcgaaga aagcggaagc
    19561 cgcgcagtaa ttctgaagcc ttgcatgtgt gtatgccgga taaggcgttt acgccgcatc
    19621 cggcatttga gcatagtacc tgatgcgacg cttgatgcgt cttatcaggc ctacaggtgc
    19681 accgcatccg gcaaaccata ctcacatcaa caacgaaaat tacccttcgt tctccgctaa
    19741 ctcgcgcaga tactggaaaa tctggcgtgc ggatttcggc ggcttattcc cttctttctc
    19801 tttcttcgcg ttacggatca gagtacgcag ttgctgacga tcggcatcgg gccacagatt
    19861 caatacttca gcgatggcgt catcaccctg gtcgatcaga cgatcgcgca ggttttccag
    19921 tttatggaac agcaccacct gctgattatg gcgatttttc agcttatcca gcgcctgacg
    19981 gatcggctca acatcgcgct ggcgcagcat cttaccgatg agctgcaact ggcggcggcg
    20041 accttccatc ttaatacgct gggcgagctc aatagccgcg cgcagatccg catctaacgg
    20101 gatcttatcc agcgcgtttt tccccagatc aacaatttcc gcgccaaggc gttttagctc
    20161 ctcggcatca cgtttaattt cacttttact gacccagata atttcatcgt cttcgtcttc
    20221 gatgtcatca ccgggaacgt cgtcgagcca gtcttcgggc tgcttagtca tctcaggctc
    20281 cttaaaaaaa gaggctaatg ttaccagtta agatgcgcac tgaaaaacgg ttctctgtta
    20341 gacttcagag aaactctcta cattatggca cttgcaatga aagtaatctc tcaagttgaa
    20401 gcgcagcgca agattctgga agaagcagtt tcgactgcgc tggagttggc ctcaggcaaa
    20461 tcggacggtg cagaagttgc cgtcagcaag accaccggca ttagcgtaag cacgcgttat
    20521 ggtgaagtgg agaatgttga atttaatagc gatggcgcgc tggggatcac tgtttatcac
    20581 cagaaccgca aaggtagcgc atcgtctacc gatttaagcc cgcaggccat tgcccgcacc
    20641 gtacaggcgg cgctagatat tgcccgttat acctcgccag atccctgtgc cggcgtggca
    20701 gacaaagagc tgctggcctt tgacgcaccg gatctcgact tgttccaccc tgcggaagtt
    20761 tccccggatg aagccattga actggcggcc cgcgcagaac aggcagcatt gcaggcggac
    20821 aaacgcatca ccaataccga aggcggcagc tttaacagcc actacggtgt caaagttttt
    20881 ggcaacagcc acggcatgtt gcagggttac tgctcaacgc gtcattcgct ctccagctgt
    20941 gtaattgccg aagaaaatgg cgatatggag cgtgattacg cctacaccat tggtcgtgcg
    21001 atgagcgatc tgcaaacgcc agagtgggtt ggggccgact gtgctcgccg cactttatcg
    21061 cgtctgtcac cgcgtaaact ctccaccatg aaagcgccgg tcattttcgc caatgaagtg
    21121 gcgaccggtc tgtttggtca cctggtcggc gcgattgccg gcggttcggt gtaccgcaaa
    21181 tccaccttcc tgctggattc tctgggcaaa cagatcctgc cggactggct gaccattgaa
    21241 gagcatccgc atctgctgaa agggctggcg tcgacgccat tcgacagcga aggtgtgcgc
    21301 accgagcgcc gcgatatcgt caaagatggc atcctgactc agtggctgct gaccagctat
    21361 tcggcgcgga aactggggct gaaaagcacc ggacatgcgg gcggtattca caactggcgg
    21421 attgccggac aagggctaag cttcgaacaa atgcttaaag agatgggcac cgggctggtg
    21481 gtgacggagc tgatgggcca gggcgtgagt gccattaccg gtgattattc ccgtggtgca
    21541 gcgggcttct gggtagagaa tggcgaaatt cagtatcctg ttagtgaaat caccatcgca
    21601 ggtaatttga aagatatgtg gcgcaatatt gtcaccgtcg gtaacgatat tgaaacacgc
    21661 agtaatatac agtgtggttc tgtgctgttg ccggagatga aaatcgccgg gcagtaaaaa
    21721 actggcgcga cctgccgcgc tataaactaa aaaattacac aaaatcattc gcactgcatc
    21781 gagacggaaa gtgaatgagg gccggggagc gtactcgcag taggtgaccc ggtcgaatga
    21841 gcgtagccgt cgatgaggca gtgtgaagga tgaagtgtaa ataaaaaagg aagtgagcaa
    21901 tgcgtaaaag cctgttagct attcttgcag tctcctcgtt ggtattcagt tcggcgtcgt
    21961 ttgccgctga tcttgaagac aatatggaaa ccctcaacga caatttaaaa gtggtcgaaa
    22021 aagccgataa cgcggcgcaa gtcaaagacg cgttaacgaa gatgcgcgcc gcagcgctgg
    22081 atgcgcaaaa agcgacgccg ccgaagctcg aaggcaaatc accggacagc ccggaaatga
    22141 aagacttccg ccacggtttc gacattctgg tcggtcagat tgacgacgcg ctgaagctgg
    22201 caaatgaagg taaagtaaaa gaagcgcagg ccgccgcaga gcaactgaaa acgacccgca
    22261 acgcctatca ccagaagtat cgttaattcc ttattgggcg gtgaaacgtt accgcccaac
    22321 gctttcttct cccttcgtaa aatcaccgca atatctatct tcaccacggc atattcctgc
    22381 gtgtcgtcct catttttgcg aggcgttttc cagaaatgaa tgtaatgcat atgtaatgct
    22441 ataatgcatt acacttttca taattaccgg aggcataatg gccacactta acgtccgcct
    22501 ggatgacaaa ctgaaaaatg aggcttatgc ggtgctggaa aaattaaata tcacgcctac
    22561 tgaagcagtg cggctgctgt ttcagtacgt tgcggaaaat ggacgaatgc cggtaaaaac
    22621 gctcactgtc agtgatagtg aagaagcctt gttacgcact gtcagggaac gactggccaa
    22681 cccacaagag gccattaagg tccgactgga tgaactatga gctggcgttt gatccgcggg
    22741 cgctgaaaga gtggcaaaaa ctgggcgtga cagtccggga acaatttaaa aaaaagcttg
    22801 aggatgtgct gaaaaggccg cgcaatccat cggctaaact gcgggattta ccggactgct
    22861 acaaaattaa gctgcgcaca cagggatatc gcctggttta tcaggttaac gataaagagt
    22921 tgctcgtgtt ggtgatagct attggtaaga gagagaactc agccgtttat gaagacgcga
    22981 ctaaacggct ggatgagtga ctaacgcaaa tgatgcacca cctgattgct gctgccgcgc
    23041 cagatcaggg aagggtcttt taaatcctgc acaaatttgc cgtcgaccag tacgttaatc
    23101 aaatcaacaa cctgcatttg cgcagcgttg agttcgtcga gtttatagcc tgtccacacc
    23161 cagatgtctt tacccggaca ttcggcgcgg atgcgttgta ccagtttcag aatatccggc
    23221 acgttttgcg gatgcagcgg atcgccgccg gagagggaaa tcccctggcg tttgatgcga
    23281 gtgtcattca gatcgttaat gatctggtct tccattgctt tggtaaatgg ctgaccggaa
    23341 tttacccgcc atgtgctttt gttatagcaa ccggggcatt catgaacaca cccggagaca
    23401 aacagggtgc agcgagtgcc ggggccgttg acgatgtcga caggatagta ctgatgaaag
    23461 ttcattttcg cttctcaatt gacgccatga ctgctctggt gcgtacgggc gaagtccccc
    23521 gatgctacgg ccaccttgcg acgacaccaa ctggccagag gcaattcatt atgtcgggaa
    23581 tcaggaaggg gcacggaacg gtaccctctc ccttgaggga gagggttagg gtgaggggga
    23641 aggattaacc tatctgccca ttccccaaat gtttaacgcg gcgcttaact tcttcctgct
    23701 taccggcgtt aaatggacgt gcatccgggc tacctaaata tccgcacacg cggcgagtca
    23761 ccgacacacg ggaggcgtca tggttaccac atttcgggca agtgaagcct ttgctggtgc
    23821 actcaaactc gccggtaaag ccacactcgt agcattcatc gatcggtgtg ttggtgccgt
    23881 aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc aggttgtgct
    23941 gaatgtttgg atactcgcca tagcaaatga agccaccgtt cgccagtggc gggtaaggcg
    24001 cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg tggaaactgt
    24061 tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagcg gtatcgagac
    24121 ggcagaagcg gtcgcacagg ttttcactcg gcgtgctgta gagactgaag ccataacccg
    24181 tttcttcttt ccactgatcc actgcctgac gcagacgttc aacaatcgca atacctttcg
    24241 cgcgcagctg ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta atggtttcgt
    24301 ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt tcagaaacgt
    24361 cgtcgtcagc attcagacgc acgccacaag cgccttccat atagaggatc ggggctacgc
    24421 gcgctttcac gccttcgaga cgagcgatac gggtcatcag cgccttacgt gccagcacca
    24481 gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcctcc agagcaatac
    24541 gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc tgctcgccgt
    24601 tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta aacgaaccgg
    24661 tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg cttgcgcact
    24721 ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc aggccatcgc
    24781 gaatcgcaaa caccagtttc gggaacaccg ccgttttacg gtttttaccg aggcctgcga
    24841 tacggttacg caggatagat tcctgaatca ggcgcgattc ccagctggtg cccagaccaa
    24901 aaccaaaggt cacaaacggc gtttgaccgt tggcggtgtg cagggtgttt acttcgtact
    24961 ccagcgactg gaaggcatcg tagcactctt tgatggtacg agagttagcg tagccttcgg
    25021 cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg gcggtgacaa
    25081 acggtgccag cacttcatcg atacggttaa tggtggtgcc gccataaata tggctggcga
    25141 cctgggcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc ggcggttcaa
    25201 tctcggcgtt ccccatttta aagccctggg tcagcatgcc tttcaggtcg atcaacatgc
    25261 agttgaacat cgggaagaac ggtgagtaat cgagatcgtg atagtgaata tcaccacgtt
    25321 catgtgcctg caccacgtca cgcggcagca ggtgctggcg tgcatagtgt ttagccacga
    25381 tcccggccag caggtcgcgc tgggttggaa tcaccttgct gtctttgttg gcgttttcgt
    25441 tgagtaacga ggagttggtc tgctcgacca gaccacggat ctcctggttc aggcgaccgc
    25501 gtttttcacg ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga gccagttgtt
    25561 tgtatggacc cgacatcagc tggttttcaa ctgcggtctg gatctcattg atatccacct
    25621 ggttgcggcc ctgcatctgc tcgctgacaa ccgcagcaac agtggcgcaa taatcggcat
    25681 catcgacttc cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc tctgatttaa
    25741 acggcacttt gcagccgtct cgtttcatca catgcggtgt catgatcgct ccatattttt
    25801 aagaacaggt tatccacaga aattgggaaa gacgttttcc ggtttttttg tttcgttttc
    25861 cggtgctttc cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt cgtggggagc
    25921 tgtagtagca attatagtcg attaatacaa catattgggt tgggacgcat tttaaagtct
    25981 atatatagtg ctttgcatca aggatgtttg aacttttttt gatgtagctc aaagtaaaaa
    26041 gcagagcgta cggatgacgg gcgctacagc gatatgtaaa ttttttaatg aattttttga
    26101 ttataaaatc aacaaaaaca acatactgac aggcaaaacc ccggaatgac aggcattacc
    26161 ggggcttagc gaagatttac ttctgtaacc accagacagc ctcaaaaggc cgtaaagtca
    26221 tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc cagttgccga
    26281 gcattttccc tggctgccag ggttgggtct cacggctaag gttggcaatg accaacaagg
    26341 tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc agcagatcct
    26401 ggtaatcgcc ccatgtcagg acggcttctt gcttacgcag tgcgattaac ttttggtagg
    26461 tgtaaaacac cgaggactca tcggtcagcg cagcttctac gttgatttcc tgatagttat
    26521 cgcccagacc aatccacggt tcgccagccg taaacccggc attatcgccg ttgctccatt
    26581 gcatgggtgt gcgactgttg tcacgggatt tactggcgag gattgccaat aactcgtcgg
    26641 catcacgacc atcgttgcgc agctcggcaa acatattgag gctctccacg tcgcgatagt
    26701 cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccctgg tagatatacg
    26761 gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc acgcggtatt
    26821 caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac cagaacaagg
    26881 cattccatgc cacgttgtgc attccttgtt gccagtggcg gaacaatgtt ttcaacgcca
    26941 caaagtcagg tttagccagc gtccatttct caccaccggg ataatcgacc ttcaggtgat
    27001 gaaaattaaa ggtcatcgac aattcactgc ctgtcagagc cgcgtatcgc tggcaatgct
    27061 caaggctggt ggaggacatt tcacctacgg tcattaacac gcgtggcgta aacacatcgc
    27121 ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag cgacgcccgt
    27181 cgccatccag gtcttcaggg taacgcggat ctttggagat cagattcacc acatccagcc
    27241 gcaacccgtc gaccccacga tcggcccaga actcacagac ttttttcagc tctgcgcgta
    27301 ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc aaatagtact
    27361 gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc cagttgttcg
    27421 gtggcgtttc tggttcgcca tcgcgccaga tataaaactg gcggtaaggg ctttctttgt
    27481 tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc atatcgagaa
    27541 tgatacgaat cccgcgcgat tttgcctgtg tcaccagttc gtcaaaatcg tccagcgtgc
    27601 cgtaggtggg atcaatcgcc gtatagttcg ctacgtcgta accgttatca acctggggag
    27661 agacataaaa gggggttagc cagatggcat caacgcccag tttatgcaga tagtccaggc
    27721 gctggataac gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga aaactctttg
    27781 gataaatctg gtagataacg ccgttttgcc accagtgggg aagatgagtc attacgttat
    27841 tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat gtccagcgtg
    27901 cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat gatgatggcg
    27961 atagccattg ccagcgcaaa cacctgccag tagctcggtt gaatcgagag aattcccggc
    28021 aggccgccta cgccgatgcc attcgccata acgccgttca ggccgcatag caatcctgcc
    28081 agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt gatgccgtac
    28141 atagcaggct cagtgactcc aagccaggcg gagatagcgg caggcacgga gatctcgcgt
    28201 tcattgtgct tgcggctgga aatgataatg cctatcacgg cggaaccctg agcgatattc
    28261 gacagcgcaa tcagcggcca cactggcgta ccacccatgc tttgaatcat ctgcaaatca
    28321 atagcaagcg tggtctgatg tacgccggtg atcaccagcg gggcgtacag gaagccaaac
    28381 aatgccgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc aaaggcaacg
    28441 ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa caccgcgagg
    28501 atcagcgaac agacgggcac caccaccaga tagaggtaat ccggcacgat gcgcttaaga
    28561 cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac ctgcgcctga
    28621 tagcccactt tggcgatgct gaacatgcca aagtcccata cttccggcag ctgctgcccg
    28681 agcagataag cgttcatcag ctgtggagaa accagcgtta cgccaagcac gataccgagg
    28741 atcggtgtac cgcccatttt tttcaccgct gaccagcaaa tcccgaccgg caggtagaag
    28801 aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga agggtacatt
    28861 tgcgccagcg tctgaccgtt gctcatgggc aaatcgccga tcacattgcg aaaaccgagg
    28921 atcaaaccac cactaatcaa cgcgggcagc aacgggaaga agatctccgc gaaatgagag
    28981 atcaactgct catgccattt catattctgc cgtgcggctt tttttacctg ctctttatca
    29041 acctgcgcct gtccggttga cgctatcagt gcttgatagt aatcacccac gttggtgcca
    29101 attaccacct gaaattgccc ggcattggtg aaacagcctt tcaccatggg gagttgctca
    29161 atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgcagacg agtaatacag
    29221 tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg atcgatatcc
    29281 gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg gttggctgca
    29341 gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacgcag cgaagatcac
    29401 aatttatcgt tcaggaaacg atcaggacag ggtggcgggg atgatgattt gttgcggttc
    29461 gctgcgcccg gttacctgcg cgatcaactg gcaggccgcc tggcgtccag cttcggcgta
    29521 accgggatct acggtgacga tctccggatg gaggaatttc attaacggcg tattaccgac
    29581 gctcgccagt tgcaaggtct cgatgcgttg ctcttgcagg tatttacttg cgccaagtgc
    29641 cagcgtgtcg gttgcgcaca gtaacgcggt agtttcaggc gtaatcactt ttgctacgtt
    29701 ctcatagcct tgcttcatag caagccctgg cagagcggca acgggatgca gtttatgcac
    29761 tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gtcgtcacgt cactatgcgg
    29821 cacgccgaga taactgatat tacgatgccc ctggtcatac agccgttgca tcaggatctt
    29881 gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac gcgccagcag
    29941 aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccggtaa aaccgaacag
    30001 cactacgccg tcgatattac gccgtttcag cacccccaaa tgttcggcaa ctaattgcgg
    30061 ggaaaactga ctttccatca tgattggatc gtaaccttgt tcatagaacg ctggcagcat
    30121 ggtttgaacg gcgagatttt ctgacaacga atccagacgg gtaacaatga tggcgaccac
    30181 tttatcgctt tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc catgctgatt
    30241 catcactgct tcaacacgct cgcgcgtgcg ctggctcacg ccgctttcgt tattcagcac
    30301 ccgggaaacc gtagatttcc ccacgccgct taagcgcgcg atgtctttga tggtcagccg
    30361 attttgcatc ctgttgtccc gtaacgtgtt gtttaattat ttgagcctaa ctttacccgt
    30421 gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca ggtattatat
    30481 cgccatagat gctacgaata ttattggatt ctccttatta tttgcggcgc ttttttcact
    30541 taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat taaaactttt
    30601 ccggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg ttttaacgtc
    30661 cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca tttctgtact
    30721 gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg gttcattcgc
    30781 catttacctt cccgtctggt tcatcgcgat ccattgcctg gcgcgcagca gacagtgaat
    30841 acggcggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat gcccgaagaa
    30901 gaattgtgga aaacgttcga cacccatccg gaaggattaa atcaggcgga agtggaatct
    30961 gcccgcgaac aacatggtga aaataaatta cccgcacaac aaccgtcgcc gtggtgggtt
    31021 catttatggg tctgctatcg caaccccttt aatattttac tcaccattct cggcgctatt
    31081 tcttacgcca cggaagattt atttgccgcg ggtgttatcg cgctaatggt cgctatttct
    31141 acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc cctgaaagcg
    31201 atggtcagca ataccgcgac ggtgctgcgc gtaattaacg acaaaggcga aaatggctgg
    31261 ctggagatcc agatcgacca gctggtgccc ggcgatatta taaaactggc ggcaggggat
    31321 atgatcccgg cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc tcaggcgtcg
    31381 ttaaccggtg agtctctgcc cgtggaaaaa gccgctacta ctcgccagcc ggagcacagc
    31441 aatccactgg agtgcgacac cctgtgtttt atgggcacca ccgtggtgag cggcacggca
    31501 caagcaatgg tgattgctac aggagccaac acctggtttg gtcaactgac ggggcgtgtt
    31561 agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt cagtatgctg
    31621 ctgattcgct ttatgctggt gatggcaccg gtggtgctgt taatcaatgg ttacaccaaa
    31681 ggcgactggt gggaagcggc gctgtttgcg ctttcggtcg cggtaggctt aacgccagaa
    31741 atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct gtcgaaacag
    31801 aaagtgatcg tcaagcatct ggatgccatt cagaactttg gcgcaatgga tattctgtgc
    31861 actgataaaa ccggcaccct gacacaggat aaaattgtgc tggagaatca taccgatatc
    31921 tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca ttaccagacc
    31981 gggcttaaaa acctgcttga tacagcggtg ctcgaaggta cggatgaaga gtcagcgcgc
    32041 tcgctggcca gccgttggca aaaaattgat gagattccgt ttgatttcga gcgtcgccgg
    32101 atgtcggtgg tagtggcaga aaataccgaa caccatcagc tggtttgcaa aggcgcattg
    32161 caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt accactcgat
    32221 gacaccatgc tgcgtaagat taagcgggtt accgatacgc tgaatcgtca ggggctgcgc
    32281 gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gcgattacca gcgagcggat
    32341 gaatccgacc tgatcctcga aggatatatt gctttccttg atccgccaaa agagacaact
    32401 gctccggcac tgaaggcatt aaaagcgagt ggcattaccg taaaaatcct tactggcgac
    32461 agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg agaggtggtc
    32521 attggtagtg atattgaaac gctatctgac gatgaactgg caaatctcgc acagcgcacc
    32581 acgctgtttg ctcgcctgac gccgatgcat aaagaacgca ttgtgacctt actgaagcgc
    32641 gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc cttacgtgct
    32701 gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc ggctgatatc
    32761 atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg acgtcgcact
    32821 ttcgccaaca tgctgaaata catcaaaatg acggcgagct ctaacttcgg taatgtgttc
    32881 agtgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt acacttgctt
    32941 attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa cgtcgacgac
    33001 gagcaaattc aaaagccgca gcgttggaat ccggcggatt tggggcgctt tatgatcttc
    33061 ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg gtgggtattc
    33121 catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gctggtttgt ggtgggctta
    33181 ctgtcgcaaa cgctgattgt gcatatgatc cgcacccgtc gtgtaccgtt tattcagagc
    33241 tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg gatcgcattg
    33301 ccgttttcac cgctggccag ttatctgcag ctgcaggcgc tgccgttaag ctatttcccg
    33361 tggctggttg cgattctggc agggtatatg acgttaaccc agttggtgaa agggttctat
    33421 agccgtcgtt acggctggca gtaaagaata aactgggcat ggtggcccga atccatattg
    33481 cagacaaagt gagctttgtt catgccggat gcggcgtaaa cgcccaatcc ggcctacaaa
    33541 ttcaataaat tgcaggaatc acgtaggcct aataagcgta gcgcatcagg cgattttgcg
    33601 tttgtcatca gacttaatcc gggcatgata gcccggattt ccatcaagat tagcgacgaa
    33661 cagcgatcgc ttcgatctca atcttcacgt ctttcggcag acgggcaact tcaacgcaag
    33721 aacgtgccgg gaaggtggcg ttgtgttcgg tgaagaaggc ttcgtaggta gcattaacgg
    33781 ttgcgaagtc gttcagatct tttacaaaca cggtagtttt aacgatgtcg cccactttca
    33841 ggccagcggc ttcgacgatc gcttttacgt tatccagcga ctgacgtgcc tgtgcagcga
    33901 cgtctgccgg tacttcgccc gttttcggat ttaccgggat ctgaccggag gtgatgatca
    33961 tattgcccag atcaacgccc tgtacgtaag gaccgatagc tgccggtgca ttttccgtcg
    34021 cgatagtttt gctcatgatt tctcctttat tacagcggta aaaagtctgg ctcattatag
    34081 ggagccagac ttttattacc aaccgcaatt aattggccag caccacatta tgagaaaact
    34141 ctttttcaca gtatttgcat ttgagcgcga tgtcattggc gcgttttcgc acggcaaagc
    34201 tggatgaaac cggttcggca tggctgatac agttgctgtt cgggcagacc agcacattgt
    34261 cgatgcgctc cggcagactt gggcgcgatt tacccaccac ttcatagttg tcgatacggt
    34321 taaccgtggc ttgcggtgca tacaatgcca gttggtctac ttgatcttca ctcaaaaagg
    34381 tattttcgat tttgatcaga tctttgcggc ccatctcgcc agaaggcagg ttcagaccaa
    34441 tggtgatgcg ctgatccgtt tcggtcagct tgaacaaact caacagctta aaaccgatct
    34501 gggcggggat atggtcaatt accgtgccgc gtttaatagc ttcaacctgc aatttattat
    34561 cgtgtgtcat ctctatttcc ccttacagta ccagatcgcg attcagaacc agtgccagta
    34621 acgcctggcg agcgaaaatc ccgttgcctg cctgctggaa gtaccaggcg tgcggcgttt
    34681 tatcaacatc ggtggcaatc tcatcaacac gcggcagcgg atgcagcact ttcatattgg
    34741 ctttggcgtt gtggagatcg ctggcgcgaa gaacaaactg cgctttcacg ttggcgtact
    34801 cggacgggtc cagacgctct ttttgcacgc gagtcatgta caggatgtct acttccgcca
    34861 tcacttcttc aatagagctg tgcagactcc aggcgatccc tttttcatcg agcatatcaa
    34921 ggatgtattg cggcattgcc aacgcgtccg gcgcgatgaa gtaaaaacgg ttgccgtcga
    34981 acttcgctaa cgcctgagtc agggagtgaa cggtgcggcc atatttcagg tcaccaacca
    35041 ttgcgacgtg gagattgtcc agacgcccct gggtttcctg aatggtgaat aagtccagca
    35101 aggtttgcgt cggatgttgg ttggagccat caccggcatt cagtaccggt acattgccgg
    35161 aaaattcggt ggccaggcgc gccgcacctt cctgcggatg acgcatcact atcgcatcga
    35221 cgtaagtgct gataaccgaa atggtatcgg ccagcgtttc gcccttttta cccagtgatg
    35281 tattggcgct gtcggagaag cccaccacgc tggcacccag gcggtgcatg gaagtttcga
    35341 aagagaggcg ggtacgggta gaagcttcga agaaacagct ggcaatgact ttgtgcttca
    35401 acagctctgg ttgcgggttt gctttcagtt tcgccgctgt cgccagcacc agattaaggt
    35461 catcgcgact aaggtcgttt atggaaatga tatgtttctg atatagcgga ttagccatct
    35521 tttatctcct gacgcctggg caaaaaaaag cccctcgatt gaggggctgg gaatgggtga
    35581 tcaacgggaa gaaaaacggc aggccagcgt cttttttcag acgcggtaag acaaaatgtc
    35641 gaacacactg aaccatacat cctcccggca aattgtccgg cattatactc atcgtcagaa
    35701 gcggtgcaag catttgatgc aatattttgt cagcgcaaac ggtttatttg aattaaaagt
    35761 caaaatacat gcatttttat ttgatgtgat tctgggggag aactgtcgtt cggtatcaat
    35821 aattgcagac atttcctgct tttttctttt tcactttcac gcaatcagat atgcatttta
    35881 ttccattcat ccgacttata gggcggagtg tgaaaacgaa cggctaacac tattgcttac
    35941 tgcgcaggga tgcgcgctat cactttaatt tcaaaatcaa agcctgccag ccaggtaaca
    36001 cccaccgccg tccagtttgg ataaggtggg gcgctaaata tttcattttt gaccgtcatg
    36061 atgtcttcaa attgtttttc tggatcggta tgaaagctcg taacatcaat gatatcgtca
    36121 aaagtgcatc ccgcagcagc cagggtcgca tgcaaattat caaaggccag tctgacttgt
    36181 tgctgaaaat cgggttctgg tgttccgtcc tctcgacttc ctacttgccc ggaaacaaac
    36241 agcaaatcgc cggaacgaat agccgcagaa taacgatgct cagcatacag tgaatgtcgg
    36301 ccagcaggga aaacagcggt tctttctacc atttggttat cctcaagatt tacgacatga
    36361 acagaagatt tatatttacc gggagccgct tttagcggac gacgtaagta aacaaagccc
    36421 agacatcatg gataatggct gggcttaatt gagcgtagtc ggttatgcgc caaacgcgcc
    36481 atcaatggta tgcatcgcgc cggtaacaaa actggcttct ggccctgcta accatgcgac
    36541 cataccagcg acctcttccg gttgcccatg tcttttgatg gccataaaac catgcaacat
    36601 atcgcgcatt ggcccgttgg cgggattagc gtcggtatct attggccctg gctggacgac
    36661 gttgatggtt atcccacgcg gtccaaaatc acgggccagc ccgcgtgcca tgccttgcag
    36721 ggcagattta ctggcggcat aagcagccat gcctgcaaca ggcatacgat cgccattcac
    36781 ggagccgatg attaagatac gcccgccttc gggcatctgc cgggcggctt caacagaggc
    36841 atgataagga gcatgaatat tgattttgaa aaggcgatca atatcgtcgg catttaattc
    36901 cagggcatcg ccaaagacgc caatacctgc atttaccacc aggatatcca atgcgccgct
    36961 cttacgaacg acatcaataa cagcgtctct gtcagcacta tctgtgaata ctgctgtcgc
    37021 tccagtctca tgtgccaggc gttcagcggc atctttcgac cccgcatagg tgaatcgtac
    37081 attggctcca tcggtgacga aacgacgtac gatagcggca ccgattccac ggctaccacc
    37141 gaggatgaga actgtcttac cggtaaaagc gcccataagg actccttgat ttattatgta
    37201 acatgcatta caaaactgtt ttaactttct gtcaacaagt tttgtaatgg tcactaaaaa
    37261 acaatctcgc gttccaggtc gtcccagacg tttcgctcct gagcaggcag tctctgcggc
    37321 aaaagtgctt tttcaccaaa aaggtttcga tgctgtcagt gttgctgaag ttactgatta
    37381 tcttggtatt aaccccccga gcctctacgc ggcttttggc agtaaggctg ggttatttag
    37441 ccgtgtactc aatgaatatg tcggtacgga agctattccg cttgccgata ttcttcgtga
    37501 tgatcgtccg gtaggcgagt gcctggttga ggtattaaaa gaagcggcgc gcagatatag
    37561 ccaaaacggc ggctgcgctg gctgtatggt tcttgaaggt attcatagtc atgatccaca
    37621 agcgcgtgat attgccgttc aatattatca cgccgcagaa acgaccattt atgactatat
    37681 cgccaggcgg catccacaaa gcgcacaatg tgtgactgat tttatgagta ccgtgatgtc
    37741 agggctttct gcgaaggcac gggaggggca ctccatcgaa caactctgtg caacagctgc
    37801 cctggcgggg gaagcgataa aaactcttat caaggagtga tgctggcctt gatccgaaag
    37861 gcgggaacgc gcctgccgat aagttgtgat aagacaataa ttcacgcgtt aaggctagcg
    37921 gaattgatca tcttttcgta taacgataga aatgaaacat tgttttaatt aaggagtgga
    37981 aaagatgatc atcggaaata ttcataatct tcagtcgtgg ctaccccagg agttacgcca
    38041 ggcgattgag catatcaaag cacacgttac ggcagaaacg cccaagggca agcacgatat
    38101 cgaaggcaat cgtctgtttt atcttatctc ggaagatatg accgagccgt acgaagctcg
    38161 ccgtgcggag taccatgccc gctatctcga cattcagatt gtgttaaaag gtcaggaagg
    38221 catgaccttc agcacgcaac ctgcaggcac cccggatacc gactggttag cagacaaaga
    38281 catcgcattt ttgccggaag gcgttgatga gaaaacagtt atccttaatg aaggtgattt
    38341 tgttgtgttt tatccggggg aagtgcataa accgctgtgc gcagtgggcg cgccagccca
    38401 ggttcgcaaa gcagtagtga agatgctgat ggcgtgatga cttttcgccg taaataactc
    38461 caggtttacg gcgagtttgt gaaaagagcg ttttttgata tttttttgtg agtaaaattt
    38521 gtaatgctta gacgttctta ttaacttaag gagttcgtca tgagcaaaat atcaggttgg
    38581 aatttttctc aacacattac atcagccgac aattttaaac aaaaaagtga agttataaat
    38641 acctggcgtg ttggaatgaa taattttgcc cgtaatgccg aggggcagga taatacaaga
    38701 aatatccttg atcctaagac atttttggag tttttggtaa aaatatttac cctgggttat
    38761 gtggatttta gcaaacgctc caatgaagcg ggaagaaata tgatggctca tattgagtcc
    38821 tcatcttata tcaaaaataa tgatggcagt gagataatga agtttgttat gaataatcct
    38881 gaaggggaac gagcagattt atctaaggtg gaaatagaaa ttacactttc cactatcact
    38941 actatgggga ctcgtcaagg acatacggct attatatttc aacaatctga tggttcgact
    39001 aatcgttatg aagggaagtc ctttgaaaga aaagatgaga gttcattaca cctgattact
    39061 aacaagattc tggcgtgtta ccaacgcgaa gctaacaagg aaatagctcg tctattaaat
    39121 attccgcagg agttaaataa ttctcatgac ttaaataact cgcaggtgag ttgtaaaggt
    39181 tcagttgatt ctacgattac ggatttatta gaaaaatcat tgaataatgc attattagca
    39241 ataaggaaag aac
//
LOCUS       DAOTRK010000023        39098 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.023, whole genome shotgun sequence.
ACCESSION   DAOTRK010000023 DAOTRK010000000
VERSION     DAOTRK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.023"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..2218)
                     /locus_tag="QIE41_001237"
     CDS             complement(<1..2218)
                     /locus_tag="QIE41_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDS3530146.1"
                     /translation="MSRRGTQFSNAKVTNPMLRIPFSSSDLGAIVNAGGGAKVLVDVT
                     AEYRQGLVRNLTTSKHYLESKLSEYPGSLGTLVFKLRDQGIAKTHRPNKIAQEAGLQN
                     AGHAKIDEMLVAAHAGCFDVLESVILHRNIKAILANLSAIERIEPWDENRKVPGGTDG
                     LFESSNILVRLFEYTGEDATYNNYENVISILEQHGVKYDEIRQKCGLPLLRIMDLSPN
                     DRYILDILIDYPGIRTLIPEPKYSAFPVSVSDSVGIETNSFPVPSEELPIVAVFDTGV
                     SPIAATITPWVVSRETYVIPPDTSYEHGTMVSSLISGAHFLNDNHPWIPDTKSKIHDV
                     CALDENGSYISDLILRLADAVNKRPDIKVWNLSLGGGPCNEQTFSDFAMELDRLSDKF
                     GILFVVAAGNYVDEPIRTWPNPDPLGGADLISSPGESVRALTVGSVSHMEANDALSEI
                     GTPTPYTRRGPGPVFTPKPDIIHAGGGVHRPWNVGASSLKVVGPDNRLCSNFGTSFAA
                     PIVASLAAHTWQRIATNTDFNVSPSLIKALLIHSAQLSSPDYSPSERRYLGAGIPNEV
                     IETLYDSDDRFTLIFQTFLVPGVRWRKDNYPIPSALIQNGKFKGEIVITAAYAPPLNP
                     NAGSEYVRANVELSFGLIENNTIKGKVPMEGENGQSGYERAQIEHGGKWSPVKIHRKA
                     FNKGITSGNWALQAKTTLRANEPALMEPLPVTIVVTLKSLDGNTQVYADGVRALNAN"
     gene            complement(2193..3347)
                     /locus_tag="QIE41_001238"
     CDS             complement(2193..3347)
                     /locus_tag="QIE41_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDS3530147.1"
                     /translation="MDYLSEVLKIIEGATKANASMASNYAGLLADKLEQKGEVKQARM
                     IRERLLRAPQALAGAQRAGGGISLGSLPVDIDSRLNTVDVSYPKLDSSEIFLPAAIST
                     RVEEFITNVQRYDEFVKADAALPSRMLVYGKPGTGKTMLSKYIATRLDFPLLTVRCDT
                     LISSLLGQTSKNLRQVFDYVMQRPSVLFLDEFDALAGARGNERDIGELQRVVISLLQN
                     MDAASEDTVIIASTNHEQLLDPAIWRRFSFRIPMPLPDIHQRELIWKNRLKNMICSDL
                     DLSDLSRKSEGLSGAIIEQVSLDARRDAVIEGASVINHHKLYRRLYLAQSLMEGVNLS
                     TYEDEIRWLRSKDKKLFSIRVLANLYKLTSRVISNILKESGAYEQKGYTV"
     gene            complement(3541..3972)
                     /locus_tag="QIE41_001239"
     CDS             complement(3541..3972)
                     /locus_tag="QIE41_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDS3530148.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPSVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEVMQRVLAFYQNGFRERLGDQG"
     gene            complement(3988..4749)
                     /locus_tag="QIE41_001240"
     CDS             complement(3988..4749)
                     /locus_tag="QIE41_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HDS3530149.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPEQAEPPEETTTATVAKISECDLTATIEQRGPQTADELATW
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAKPKAASVTETDFKAFPQ
                     PADVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLILPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIADSSRRVVSEK"
     gene            complement(4772..5518)
                     /locus_tag="QIE41_001241"
     CDS             complement(4772..5518)
                     /locus_tag="QIE41_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS3530150.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEAQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            complement(5525..6382)
                     /locus_tag="QIE41_001242"
     CDS             complement(5525..6382)
                     /locus_tag="QIE41_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HDS3530151.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIAED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVEVVFEHDVNTKAT
                     NKDTDKDLKTDPPLNPPRGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRT
                     EQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPD
                     SAIPPGFRG"
     gene            complement(6395..6817)
                     /locus_tag="QIE41_001243"
     CDS             complement(6395..6817)
                     /locus_tag="QIE41_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530152.1"
                     /translation="MKIKHEHIESVLFALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(6840..7136)
                     /gene="ydaS"
                     /locus_tag="QIE41_001244"
     CDS             complement(6840..7136)
                     /gene="ydaS"
                     /locus_tag="QIE41_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaS"
                     /protein_id="HDS3530153.1"
                     /translation="MKKENYSFKQACAVVGGQTAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATRGEVLCEELRPDIDWSYLRRSACCSQNMSVKQLNDSNKSSFDHT"
     gene            7260..7736
                     /gene="racR"
                     /locus_tag="QIE41_001245"
                     /gene_synonym="rarC"
     CDS             7260..7736
                     /gene="racR"
                     /locus_tag="QIE41_001245"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HDS3530154.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGSVKSKAEVARHFKVQPPSIYDW
                     IKKGSISKDKLPELWRFFADVVGPEHWGLNEYPIPTPTNPDTKSELLDINNLYQAASD
                     EIRAIVAFLLSGNATEPDWVDHDVRAYIAAMEMKVGKYLKALESERKSQNITKTGT"
     gene            8190..8342
                     /locus_tag="QIE41_001246"
     CDS             8190..8342
                     /locus_tag="QIE41_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDS3530155.1"
                     /translation="MQKIDLGNNESMVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LARNTC"
     gene            8763..8984
                     /gene="kilR"
                     /locus_tag="QIE41_001247"
     CDS             8763..8984
                     /gene="kilR"
                     /locus_tag="QIE41_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HDS3530156.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDCMIKVFLGRNGLPVKAESW"
     gene            8984..9154
                     /locus_tag="QIE41_001248"
     CDS             8984..9154
                     /locus_tag="QIE41_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530157.1"
                     /translation="MTKKIKCAYHLCNKEIEESKSIKRPLHFMRGVIQTTEMKKYCSE
                     SCAEKDQMAHEL"
     gene            9229..9504
                     /locus_tag="QIE41_001249"
     CDS             9229..9504
                     /locus_tag="QIE41_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530158.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLHEGVKKMCLVHTGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            9606..12257
                     /gene="recE"
                     /locus_tag="QIE41_001250"
     CDS             9606..12257
                     /gene="recE"
                     /locus_tag="QIE41_001250"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HDS3530159.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETNELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQREHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDIATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTAYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKLQPSGTTADEQGEAETMEPDATKHHQDTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKTSPDMKQPEPVVQQEPEIAFNACGQTGGDNCPDCGAVMGDATNQETFDEENQ
                     VEAKENDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
                     YHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRF
                     IVAPEFNRRTNAGKEEEKAFLMECASTGKMVITAEEGRKIELMYQSVMALPLGQWLVE
                     SAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQ
                     DAFYSDGYEAQFGVQPTFVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTL
                     ADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            12250..13059
                     /gene="recT"
                     /locus_tag="QIE41_001251"
     CDS             12250..13059
                     /gene="recT"
                     /locus_tag="QIE41_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HDS3530160.1"
                     /translation="MTKQPPIAKADLQKTQENRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRRQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            13116..13310
                     /gene="ralR"
                     /locus_tag="QIE41_001252"
     CDS             13116..13310
                     /gene="ralR"
                     /locus_tag="QIE41_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HDS3530161.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            13303..13491
                     /locus_tag="QIE41_001253"
     CDS             13303..13491
                     /locus_tag="QIE41_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HDS3530162.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAAVS
                     REQKVKLINFNCERLQSS"
     gene            13591..13806
                     /gene="xisR"
                     /locus_tag="QIE41_001254"
     CDS             13591..13806
                     /gene="xisR"
                     /locus_tag="QIE41_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HDS3530163.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            13808..15043
                     /locus_tag="QIE41_001255"
     CDS             13808..15043
                     /locus_tag="QIE41_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDS3530164.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRSLIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILIYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            15095..16030
                     /gene="ttcA"
                     /locus_tag="QIE41_001256"
     CDS             15095..16030
                     /gene="ttcA"
                     /locus_tag="QIE41_001256"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDS3530165.1"
                     /translation="MKENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(16159..17532)
                     /gene="dbpA"
                     /locus_tag="QIE41_001257"
     CDS             complement(16159..17532)
                     /gene="dbpA"
                     /locus_tag="QIE41_001257"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDS3530166.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLETEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGRIKGKT
                     CRVRLLK"
     gene            complement(18010..18993)
                     /gene="zntB"
                     /locus_tag="QIE41_001258"
     CDS             complement(18010..18993)
                     /gene="zntB"
                     /locus_tag="QIE41_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDS3530167.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            19248..20480
                     /gene="dgcM"
                     /locus_tag="QIE41_001259"
     CDS             19248..20480
                     /gene="dgcM"
                     /locus_tag="QIE41_001259"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDS3530168.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCTLARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(20501..21064)
                     /gene="smrA"
                     /locus_tag="QIE41_001260"
     CDS             complement(20501..21064)
                     /gene="smrA"
                     /locus_tag="QIE41_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDS3530169.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(21259..22773)
                     /locus_tag="QIE41_001261"
     CDS             complement(21259..22773)
                     /locus_tag="QIE41_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDS3530170.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFDR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(22770..23798)
                     /locus_tag="QIE41_001262"
     CDS             complement(22770..23798)
                     /locus_tag="QIE41_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDS3530171.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSTGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            23895..24710
                     /locus_tag="QIE41_001263"
     CDS             23895..24710
                     /locus_tag="QIE41_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3530172.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(24883..25791)
                     /locus_tag="QIE41_001264"
     CDS             complement(24883..25791)
                     /locus_tag="QIE41_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3530173.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKHLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKATYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            25967..27277
                     /gene="abgA"
                     /locus_tag="QIE41_001265"
     CDS             25967..27277
                     /gene="abgA"
                     /locus_tag="QIE41_001265"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDS3530174.1"
                     /translation="MESLNQFINSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALEQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEERDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFERAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVPGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            27277..28722
                     /gene="abgB"
                     /locus_tag="QIE41_001266"
     CDS             27277..28722
                     /gene="abgB"
                     /locus_tag="QIE41_001266"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDS3530175.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQASCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYRALSHFGTPEWNCEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            28759..30285
                     /gene="abgT"
                     /locus_tag="QIE41_001267"
     CDS             28759..30285
                     /gene="abgT"
                     /locus_tag="QIE41_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDS3530176.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            30296..30811
                     /gene="ogt"
                     /locus_tag="QIE41_001268"
     CDS             30296..30811
                     /gene="ogt"
                     /locus_tag="QIE41_001268"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDS3530177.1"
                     /translation="MLRLLEEKIVTPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            31006..31758
                     /gene="fnr"
                     /locus_tag="QIE41_001269"
     CDS             31006..31758
                     /gene="fnr"
                     /locus_tag="QIE41_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDS3530178.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            31910..32860
                     /gene="uspE"
                     /locus_tag="QIE41_001270"
     CDS             31910..32860
                     /gene="uspE"
                     /locus_tag="QIE41_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDS3530179.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(32910..33167)
                     /locus_tag="QIE41_001271"
     CDS             complement(32910..33167)
                     /locus_tag="QIE41_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDS3530180.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            33411..34442
                     /gene="ynaI"
                     /locus_tag="QIE41_001272"
     CDS             33411..34442
                     /gene="ynaI"
                     /locus_tag="QIE41_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDS3530181.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(34493..36106)
                     /gene="mppA"
                     /locus_tag="QIE41_001273"
     CDS             complement(34493..36106)
                     /gene="mppA"
                     /locus_tag="QIE41_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDS3530182.1"
                     /translation="MKHSVSVTCCALLISSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGKLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(36443..37342)
                     /gene="pgrR"
                     /locus_tag="QIE41_001274"
     CDS             complement(36443..37342)
                     /gene="pgrR"
                     /locus_tag="QIE41_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDS3530183.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            37480..38400
                     /locus_tag="QIE41_001275"
     CDS             37480..38400
                     /locus_tag="QIE41_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS3530184.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            38471..>39098
                     /locus_tag="QIE41_001276"
     CDS             38471..>39098
                     /locus_tag="QIE41_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS3530185.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKP"
BASE COUNT        10696 a         9441 c         8854 g        10107 t
ORIGIN      
        1 tattagcatt taaagctctt acgccatcag cataaacttg tgtgtttcca tctaatgatt
       61 ttaaagttac tacaatagtt acaggtaaag gctccattaa ggccggttca ttcgctctca
      121 acgttgtttt agcctgaaga gcccagttac ccgaagtaat tcctttatta aatgccttgc
      181 gatgaatttt tactggtgac cactttccac catgctcaat ttgagctctc tcatatccag
      241 attgaccgtt ttctccttcc ataggcactt ttccttttat agtattattc tcaattaagc
      301 caaaacttag ctctacgttc gcgcgaacat attcactgcc ggcattaggg ttcagtggtg
      361 gtgcatatgc agcagtaatt acaatctcac ctttaaattt tccattttga ataagtgccg
      421 atggtatggg atagttatcc tttctccacc tcaccccagg aaccaagaat gtttggaaaa
      481 tcagagtaaa cctatcatca ctatcatata aggtctcaat aacttcatta ggaattcccg
      541 ctcccaaata gcgtctttca cttggcgagt aatcaggaga agataattga gcggaatgaa
      601 ttaataatgc tttaatcaat gatggtgaaa cattaaagtc tgtattagtg gctattctct
      661 gccatgtatg cgcagctaaa cttgccacaa ttggagcagc aaaactagta ccaaaattag
      721 agcaaagcct attatctggc cctacgacct ttaaactgct tgctcctaca ttccaaggtc
      781 tatgaacccc accgccagca tggattatat ctggctttgg agtaaataca ggcccagggc
      841 cacgacgagt atatggtgtc ggtgttccaa tttcacttaa agcatcatta gcttccatat
      901 gagaaactga accaactgtt agtgctcgga ctgactctcc aggagaggaa attaaatcag
      961 cacctccaag cggatcagga tttggccatg tacgtatagg ttcatctaca taattacctg
     1021 cagcaactac aaacaaaata ccaaatttat cgctgagccg atctaactcc atcgcaaaat
     1081 cactaaacgt ctgctcatta catggtccgc ctcccaaaga caaattccag acttttatat
     1141 ctggtctttt atttacagca tctgctagcc tcagaattaa atctgatata taagatccat
     1201 tttcatctaa ggcacaaaca tcatggattt tagattttgt atcaggaatc catggatgat
     1261 tgtcatttaa aaaatgagcg cctgatatca atgaagacac catagtccca tgttcataac
     1321 tcgtatcagg aggaattacg tatgtttccc tactcactac ccaaggagta attgttgccg
     1381 cgatggggct taccccagtg tcaaatacag caacaatggg taattcttct gatggtacgg
     1441 gaaagctatt tgtttcaatg ccaacagaat cacttacact aaccgggaat gctgaatatt
     1501 ttggttcagg aattaacgtt cttatacccg ggtaatcaat gagaatgtct aatatatatc
     1561 tatcatttgg ggataaatcc attatcctta ataagggaag accacatttt tgtctaatct
     1621 catcatattt aactccgtgt tgttctaata tagaaataac gttttcatag ttgttgtaag
     1681 ttgcatcttc acctgtgtac tcaaatagtc gtacaaggat gtttgatgat tcaaacaaac
     1741 catcagtgcc tcctggaacc ttcctattct catcccaagg ttcaatgcgc tcaatcgcgc
     1801 ttagattagc caaaatcgct ttaatattcc gatgtaaaat gactgactct aatacgtcaa
     1861 aacagccggc atgagcagca accaacattt catctatttt ggcatgaccg gcattttgca
     1921 atccagcctc ttgagcaatt ttgttcggcc tatgcgtttt ggctattccc tggtctctta
     1981 atttgaaaac caaagtaccc aagcttccag ggtactctga aagtttggat tctaaataat
     2041 gtttactggt tgttaaattt cttactagcc cttgtctata ttcggctgtt acatcaacca
     2101 atacctttgc cccaccgcca gcgtttacta ttgcacccaa gtcactactg gaaaaaggga
     2161 ttcttaacat tgggtttgta acttttgcgt tactaaactg tgtacccctt ctgctcatat
     2221 gctcctgact ccttcagaat gtttgaaatt actcttgatg taagtttgta caaattagca
     2281 agaactctga tagaaaataa ttttttatct ttagaacgta accaacgaat ttcatcttcg
     2341 taagtgctta aatttacacc ttccataagc gattgagcaa gatacaaacg cctatacaat
     2401 ttatggtgat ttatcacact tgcaccttca ataactgcat ccctacgtgc atccaagctc
     2461 acctgttcaa ttattgctcc ggataatcct tccgattttc ttgataaatc acttaaatct
     2521 agatcgctac atatcatatt ctttaaacga tttttccaaa ttaactctct ctgatgtatg
     2581 tcaggcagag gcattggaat tctgaagcta aatcgcctcc agattgcagg atccagaagt
     2641 tgctcatggt tagttgaggc aataattacc gtatcctctg atgccgcatc catattctgc
     2701 aatagtgaaa tgacaactcg ctgaagctca cctatatctc tctcattacc tcttgctcca
     2761 gctaaagcat caaattcgtc taaaaaaagc actgatggcc tctgcattac ataatcgaaa
     2821 acctgtctaa gatttttgct ggtttgtccc aataaactac taatcaaagt atcgcaacgc
     2881 actgtaagaa gtggaaaatc taagcgggta gcgatgtact tagataacat agtcttacct
     2941 gttcctggct ttccatacac gagcatacga ctcggcaatg ctgcatcagc tttaacaaac
     3001 tcatcataac gttgaacatt agtgataaac tcttcaacac gggtactgat tgctgcaggc
     3061 agaaaaatct ctgaactgtc taatttagga taactgacat caacagtgtt gagtcgacta
     3121 tcaatatcta ccggtaatga gcccagagat atcccacctc cagccctttg agctcctgcc
     3181 aacgcctggg gagctctaag caacctttct cttatcattc tggcttgctt gacctcccct
     3241 ttttgttcga gcttatctgc cagcaaccca gcataattac tagccatcga agcatttgcc
     3301 tttgttgcac cttcaatgat ttttaacact tctgataaat aatccattca acactcccac
     3361 aacaaaaagc cacagcaatc aaaaaatgag cacgcagaat caaaatactc tctaatcgta
     3421 acgcattttg agatttttga tacgtttttt tgggattatg taacgccaaa tggtcatttt
     3481 gtgtcagaaa aataaatctt agtaccgaga atatgtagct tccatccttc atcgttctat
     3541 tcatccctga tctccaagcc tctctcgaaa accattctga taaaacgcca gcacacgctg
     3601 cataacttca ctcttccggc actcgcgaca gattatgttc agacgcctgt cgtagcggcg
     3661 tatttctccg tctggtaatg accagataag gtccggatca accacagatg gtttcttcac
     3721 ctttgccctg gatagttttt tgcgggcatt ttgccagtcc ttacgagcct gttcagacgg
     3781 gaataacccg taaccagagt tgtatacatc gccactggca accagctctc tggcgagaac
     3841 gctcatcaga tatcttgtcg cacctgtctt ggcctccagt tgtcgtaacg tctcgcgtcc
     3901 actgcggcgt actagctcaa caacctgccc tttaattttt tcccgctctt cttgtgtaaa
     3961 tacttttgcc ataagcgcct ccggcaatca cttttccgat acaacacggc gggaagaatc
     4021 agcaatctgt cgaacaatat cccggtgctt gttcagctcc cgcagcgcgg cgcagactcg
     4081 ctcccacttc tggacatgat ttttcgcccg acgcagttcg cggtttgcca tatgcagcga
     4141 tggcaaaatc aggtcatcag ctcgcgtttc agtaaacgat ggcagcgact gcacaatgtc
     4201 cgccacagtt tctgttttaa tatcttcctg tgttgcagct tcctgtactg gtaacgcaac
     4261 atctgctggc tgaggaaagg ctttaaaatc agtttccgtt accgatgcag ctttcggctt
     4321 tgctggtaaa ttatcgcccg gtatgcagta acgaaattta ccgttctgat taacgcgaat
     4381 cagacgacct ttgctgattg ccattgccag cgttgaagcc actttgcgtg atgtggtacc
     4441 gaaccatgta gccaactcat ccgccgtttg tggtccacgt tgttcaatcg tcgcagttaa
     4501 atcgcactcc gaaattttcg cgactgttgc cgtggtggtt tcttccggcg gttctgcctg
     4561 ctctggctgt tcctgctgaa cgttgttatc agccacacgc caggtgtata cgcttttatc
     4621 aacgaaacca gcctttttca gctcccacag ctcgttcagt acctcttcac gactgatatc
     4681 aagtcgcgca gccagttcta ccgacgtggc ttttcccatt gctttcagtg cgtcaaaaac
     4741 agtctccatt aaaatttcct cccggtaaaa atcacttcgc aattcctggc tggacgacat
     4801 tcggacgcca gctctcccag ttaaaattca cccagcgtcc gccgttcatg gtcatgcgat
     4861 ccataatccg ctcgccgagc aatgttttca tggcctcata gttcaggttt gtcagcatcc
     4921 ccacgctgcg catcgacgct gtccggcgat caacaatctg gtgcagcacc acctgctcgt
     4981 tttttgtctc gcgctgaatg ccaatttcat caagaaccag cagatccact tcgcacagtt
     5041 cccgcaaaaa tttttcgcct gactgcccat cgtcatagct ggcgtgcagg gcactcataa
     5101 catcagccac ggtaaccaca atcactgtct gaccgtcttt cagcaggcga ttcccgatag
     5161 ctgccgctaa gtggttcttc ccggtaccag gttttccgct gaacgcaaaa tttgtacacc
     5221 cggtcatcag ttcatcagcg atggatttcg cctgactcaa cgcgtatcgc tgcgcttcgt
     5281 tctgcacctg gtaattcgaa aacgagcatt tgcggtgcaa tggctggatg ccagagcgat
     5341 tcagaatttt ttccacccgc aactgacgat tctgacggtt gatctcctca caacgtttct
     5401 ggccttcgga aagttgccac tcgcgccact ccgctaccgt cttgaatggc gcggttacat
     5461 gtgacggggc cagtctgcgg atacgttcaa gaacgtcgcc tgtcgcaata tttttcatgg
     5521 acagttaccc cctgaagcct ggcgggatcg cactatccgg taacgagacg gtgttaacct
     5581 gtcggagtaa cgtctcaggt cgaacacctt tcggcgcgaa caagccctgg tattcattgg
     5641 cgatgctgtg tctaatcacc tgctcaggtg aaaaaccctg ctggcggaat ttttccagct
     5701 cccgtatcgc cccgttagcg ccctgctccg ttcgaatcgg ttttcgcaat gcctggcgaa
     5761 attcaaccca ctcacgccaa agcgagacag aaatccagtt cggcaaagca atatccagag
     5821 ggtcaaactt tttgacacct cgattccccc gggggggatt taggggggga tctgttttta
     5881 gatctttatc tgtatcttta ttagttgcct ttgtgttgac atcatgttca aacaccactt
     5941 caacatctgt ttgaacacct gttaaatttc tctcttgttt tgtttgaaca tctgcttcct
     6001 ttctgcttct tctggcctga acagatgctt ttcctgcggc tgattttttg gttaattttt
     6061 ccctgactga tgccagatct tccgcaatcc gaagatgcac ccattcctcg ccgttatcgc
     6121 aaaaaaactc ctgcaaggat ggttcaacat cagcccatcg ctcgttagtc agacgggcaa
     6181 tttttgccag cctgttttta ggtattggct ttcctgtttg ccagtaattg aacatcagca
     6241 acaaatacgc accatgctcc tctgctgaca aatgcatggt gtcagccagg taatcagcta
     6301 tgtacagttg catgtatggt aatgcggcca taattgcccc gtatgatgct gcccggtggc
     6361 ttagaataag cacaaacagc atgaaaactt ttgcttaatg aacaatgaca gaatcgtcgg
     6421 aagacccgcc gccgctgaaa tgcgctttcc ggtaaacggc ttggactgca tcatcatgcg
     6481 catcaattgc cgtacttaac gcttcctgcg ccgccagtaa tgcacggcgt tccagggtat
     6541 cgaagatgca gagtcggtga cgcagctcgc gcggaaggat tgccagaatt gctgggatca
     6601 gcttctgaat tttttccctt tgcgcattcg tttcaccttt caaccaacgg tgatagatat
     6661 tctgctgatt gttccagtcc ttgcctggaa ccaggggcaa ttcgccgccc ccctggcgca
     6721 gatattcttc agtaattgca ttggctaccc atgcctgccc tttttcggct gccagggcaa
     6781 acaacaccga ttcgatgtgc tcatgcttga ttttcatgaa tcatttgcct cttgatgttt
     6841 caggtatgat caaatgagga tttgttactg tcatttagtt gcttcactga catattctgc
     6901 gaacaacatg ccgaacgtcg taaatatgac cagtcaatat caggacgaag ttcttcgcac
     6961 agcacctcac ctcttgttgc acgttcaatt gctggacatc tctcggcagg caattgacgt
     7021 acccctttga tccattgatt tacgcttgga ggtgatacac ctaaaagcct agccattgct
     7081 gtttgcccac cgacaacagc acaagcttgc ttgaatgaat agttctcttt tttcatcgaa
     7141 tgaactccaa aaacacacag agatattagg cgacgcctaa tacaaatgtc aataggctat
     7201 gcctaataca atgagagtag ggattgccta atgcaatgag cataggagaa tattaagcaa
     7261 tgcttagtgg taaagactta ggccgagcga tagagcaggc cattaacaaa aaaatcgcat
     7321 cgggatccgt caaatcaaag gcggaggtcg cacgccactt caaagtccaa ccaccatcaa
     7381 tctatgactg gattaagaaa ggctccataa gtaaagataa acttccagaa ttatggcgtt
     7441 tctttgctga tgttgttggt ccagagcatt gggggcttaa cgaatacccc ataccaaccc
     7501 ccaccaatcc agatacaaaa agtgaacttt tagatataaa caacctttat caagcagcct
     7561 ctgatgaaat aagagcgatt gtagctttcc tgttatctgg aaatgctaca gaaccagatt
     7621 gggttgacca cgatgttcgc gcctacatag cagcgatgga aatgaaagtg ggtaagtatc
     7681 tgaaagctct agaatctgaa cggaaaagcc agaacatcac aaaaactgga acttaaactt
     7741 atatggtctg acggaaaact cctggattcc gttatttaac ccccccatca ctttctccag
     7801 tcgccatcac ctattaggtt acgcttaaaa cattaggcat agcctattga caatcaatta
     7861 ggcattacct atagttccag cataccaccc accccgcccc acagaacgcc aggcaatact
     7921 tcgagttacc aggcagtggt caggggttaa gtagccagcc cgaggcgtat gaacatgacg
     7981 gcgggattca aattttgcag tgcagcagtt agttccgcca cccggcgtta aggggagaga
     8041 taagatggtg cattacgaag tagttcagta tttgatggat tgttgcggta tcacttacag
     8101 ccaggctgtg caggctttac gtagcaacga ctgggatctc tggcaagcag aagcctctat
     8161 ccgcaacaac aagatgtgag gtgcgaaaaa tgcaaaaaat cgacctcggc aataacgaat
     8221 ccatggtgtg cggcgtgttc cctaaccaag atggaacgtt cactgctatg acgtatacca
     8281 aaagcaaaac atttaaaaca gaaacaggcg cacgtcgctg gcttgccaga aatacttgct
     8341 aatccattat ttggattaat tcaatattct cgctgtaggg gtatagcaga aaccaccaaa
     8401 gcccggaggt ggtgaaataa aaccgggcac aacacgaagg cgcatttccg atatccataa
     8461 agagtcggtc ttgtctgtta aatttaaatg gtgggagtgc gcctccggtt gtggataaca
     8521 acactgctat gtgtagtctt ggcggcatca gttttttctt gaagttcgac tgatgcccgc
     8581 cctttttaaa gtgaattttg tgatgcggtg aatgcggcta agcgcacgcg gcacagttaa
     8641 aagtcatgtt agtccttatt ggtttgggtg ggaaagccga ctgtaattgt taactggttg
     8701 cagtcacctg gaggcaccag gcaccgcatc aacaaagttc atttgtaaaa atggagataa
     8761 ttatgattgc acatcacttc ggaactgatg aaataccacg tcagtgtgtg actcctggtg
     8821 actatgttct tcatgaaggc cggacatata ttgcctcggc aaacaatatt aaaaagcgaa
     8881 aactttatat tcgtaacctg accacaaaaa catgcattac tgactgcatg attaaagtct
     8941 tcctcggtcg taatggttta cctgtaaagg cggagtcatg gtgatgacta agaaaataaa
     9001 atgtgcttac cacctttgca ataaagaaat tgaagaaagc aaaagtatca aaagaccact
     9061 tcatttcatg cgtggagtta tccaaacgac ggaaatgaaa aaatattgta gtgaaagttg
     9121 tgccgaaaaa gaccagatgg cacacgaact ttaattaact gactatgcga aactgaattt
     9181 atgccagcaa tggcagggat tcgctcaacc ttaattaagg agaaaaacat gattaccagt
     9241 tatgaagcca ctgttgtaac tactgatgac attgttcacg aggttaatct ggaaggaaag
     9301 cgtattggct acgtgattaa aacagaaaat aaagaaaccc cattcactgt ggttgatatc
     9361 gacggtccat caggcaacgt taaaacactt cacgaaggtg tcaaaaaaat gtgcctggtg
     9421 cataccggaa agaatctgcc cgcagaaaaa aaagccgaat ttctggcaac tctaattgca
     9481 atgaaattaa aaggtgaaat ctgaaagaaa tagcctgcgt atggcgcagg ctatgaacag
     9541 tgtgtatccg gcaagatcat tcactgaaca aaacgaattt taatctgagt tgaggttaaa
     9601 aaacaatgag cacaaaacca ctcttcctgt tacggaaagc gaaaaaatca tccggtgaac
     9661 ctgacgtcgt cctgtgggca agcgacgatt ttgaatcgac ctgtgccact ctggactacc
     9721 tgatcgttaa gtcaggtaaa aaactgagca actattttaa agctgttgcc acaaattttc
     9781 ctgtcgttaa tgaccttccc cctgaaggtg agatcgattt tacctggagt gaacgctatc
     9841 aactcagcaa agactccatg acatgggaac taaaaccggg agcagcgcca gacgacgttc
     9901 accaccagga taatgctcaa gaaaccaacg aactggcggg aggccaggaa gaaaacgcgc
     9961 aggcagacgc ccacgaggat tgccaggatt gcgaagtctc tgtagccact ttgcggttca
    10021 cacagcgtct tctgcacatt tttacgtatg cggccgggga tcggaaatac ctgcatcatg
    10081 ccacccgaga acaacgcgaa cacattactg ctcttgagat ggatcaggaa aacagctatg
    10141 tccagaatct gctgttggcc atacgcggca tggcagaacc gacaactctg gataatgccg
    10201 ccctgctccg cctgactgat gcaattaagg cagttttctc tatcactaaa aaacatcagc
    10261 cctatgaatt taagaatttc atttcagcct ggctggatac cgaacacatt gatcgcggtc
    10321 ttctgacaaa agaatggcga aaagggaatc gtgtttcacg catcactcgc acggcttccg
    10381 gtgctaatgc tggcggcggg aacctcacag atcgcggcga aggtttcgtc cacgatctga
    10441 cgtcactggc gcgcgatata gccactggcg tactggcccg ttcaatggac gtggacatct
    10501 ataaccttca tccggcacac gctaaacgca ttgaggaaat tatcgctgaa aataaaccgc
    10561 ccttttctgt tttccgcgac aaattcatca ccatgcctgg cgggctggat tattcccgcg
    10621 ccatcgtggt tgcgtccgtg aaagaagcac caattgggat cgaggtcatc cccgctcacg
    10681 tcactgcata tctgaacaaa gtactgactg aaaccgatca tgccaaccct gatccggaaa
    10741 tcgtggatat tgcctgcggt cgctcctctg ccccgatgcc gcagcgagta acagaagaag
    10801 gaaaacagga tgatgaagaa aaactgcaac catctggaac aacggcagat gaacagggag
    10861 aggctgaaac aatggaaccg gacgcaacta aacatcatca ggacacgcag ccgctggatg
    10921 ctcagtcaca ggtaaattct gttgatgcga aatatcagga actgcgggca gaactccatg
    10981 aagctcggaa aaacattcca tcaaaaaatc ctgtcgatgc cgataaattg cttgctgcat
    11041 cacgtggtga atttgttgac ggaattagcg acccgaacga tccgaaatgg gttaagggga
    11101 tccagactcg cgattctgtg taccagaacc agccagaaac ggaaaaaacc agcccggata
    11161 tgaaacaacc tgagccagta gtgcaacagg aaccggaaat agccttcaat gcctgcggcc
    11221 agactggcgg ggataactgc cctgactgtg gtgcggtgat gggcgacgca acaaaccagg
    11281 aaacattcga tgaagagaat caggttgaag ctaaggaaaa tgatccggag gaaatggaag
    11341 gcgctgaaca tccgcacaat gagaatgctg gcagcgatcc gcatcgcgat tgcagtgatg
    11401 aaactggcga agtcgcagat cccgtaatcg tagaagacat agagccaggt atttattacg
    11461 gaatttcgaa tgagaattac cacgcaggtc ccggtgtcag taagtctcag ctcgatgaca
    11521 ttgctgatac tccggcactg tatttgtggc gtaaaaatgc ccccgtggac accacaaaga
    11581 caaaaacgct cgatttagga accgctttcc actgccgggt acttgaaccg gaagaattca
    11641 gtaaccgctt tatcgtagca cctgaattta accgccgtac aaacgccgga aaagaagaag
    11701 agaaagcgtt tctgatggaa tgcgcaagca caggaaaaat ggttatcact gcggaagaag
    11761 gccggaaaat tgaactgatg tatcaaagcg ttatggcttt gccgctgggg caatggcttg
    11821 ttgaaagcgc cggacacgct gaatcatcaa tttactggga agatcctgaa acaggaattt
    11881 tgtgtcggtg ccgtccggac aaaattatcc ctgaatttca ctggatcatg gacgtgaaaa
    11941 ctacggcgga tattcaacga ttcaaaaccg cttattacga ctaccgctat cacgttcagg
    12001 atgcattcta cagtgacggt tatgaagcac agtttggagt gcagccaact ttcgtttttc
    12061 tggttgccag cacaactatt gaatgcggac gttatccggt tgaaattttc atgatgggcg
    12121 aagaagcaaa actagcaggt cagctggaat atcaccgcaa tctgcgaacc ctggctgact
    12181 gcctcaatac cgatgaatgg ccagctatta agacgttatc actgccccgc tgggctaagg
    12241 aatatgcaaa tgactaagca accaccaatc gcaaaagccg atctgcaaaa aactcaggaa
    12301 aaccgtgcac cagcagcaat taaaaataac gacgtgatta gttttattaa ccagccatca
    12361 atgaaagagc aactggcagc agctcttcca cgccatatga cggctgaacg tatgatccgt
    12421 atcgccacca cagaaattcg taaagttccg gcgttaggaa actgtgacac tatgagtttt
    12481 gtcagtgcaa tcgtacagtg ttcacagctc ggacttgagc caggtagcgc cctcggtcat
    12541 gcatatttac tgccttttgg taataaaaac gaaaagagcg gtaaaaaaaa cgttcagcta
    12601 atcattggct atcgcggcat gattgatctg gctcgccgtt caggtcaaat cgccagcctg
    12661 tcagcccgtg ttgtccgtga aggtgacgaa tttaatttcg aatttggcct tgatgaaaag
    12721 ttaatacacc gcccaggaga aaacgaagat gccccggtta cccacgtcta tgctgtcgca
    12781 agactgaaag acggaggtac tcagtttgaa gttatgacgc gcagacagat tgagctggtg
    12841 cgcagccaga gtaaagctgg taataacggg ccgtgggtaa ctcactggga agaaatggca
    12901 aagaaaacag ctattcgtcg cctgttcaaa tatctgcccg tatcaattga gatccagcgt
    12961 gcagtatcaa tggatgaaaa ggaaccactg acaatcgatc ctgcagattc ctctgtatta
    13021 accggggaat acagtgtaat cgataattca gaggaataat tcagtctggc ggtgtaatgc
    13081 accgccaact tgaaatattt tttatgagaa aaattatgag atatgacaat gttaaaccat
    13141 gtccattttg tggttgtcca tcagtaacgg tgaaagccat ttcaggatat taccgagcga
    13201 agtgtaacgg atgcgaatcc cgaaccggtt atggtggaag tgaaaaagaa gcactcgaaa
    13261 gatggaataa acgaaccact ggaaataata atggaggtgt tcatgtataa aattaccgcc
    13321 actattgaaa aggaaggtgg cactcctact aactggacaa gatattcaaa atctaaacta
    13381 acgaaatcag aatgcgaaaa aatgctctca ggtaaaaaag aagcagccgt ttccagagag
    13441 cagaaagtaa aactgataaa ttttaattgc gagagacttc agtcctcgtg aattgcattg
    13501 tattcaaatt aaaacttcat agctgattat taataatcaa catcgggcgt caatttcagt
    13561 ctaacattgg cgcctgccag aggtgatgcg atggcacaag taatctttaa tgaagagtgg
    13621 atggttgaat acggcctgat gcttcgcact ggtctggggg ccagacaaat tgaagcatac
    13681 cgccagaact gttgggtgga aggcttccac ttcaaacgag tatctccttt agggaagcca
    13741 gacagtaagc gagggattat ctggtacaac tatccaaaga taaatcagtt tatcaaagac
    13801 tcatgatatg tctaaattac caacaggtgt cgagattaga ggtagataca ttcgcatctg
    13861 gttcatgttt cgaggaaaac gatgtcggga aacattaaaa ggctgggaga ttacaaacag
    13921 taatattaaa aaggccggaa atttaagatc gctgatagtt catgaaataa actccggtga
    13981 atttgagtat ttaagacgtt ttccccagtc cagcactggg gcaaaaatgg tgacaacgag
    14041 agtcataaaa acgttcggag aactttgtga tatctggaca aaaattaaag agacagagtt
    14101 aacaacaaac acaatgaaga aaacgaaatc acaattaaaa acactcagaa taataatttg
    14161 tgaaagtacc ccgatatcac atattcgtta tagcgatatc ttaatctacc ggaatgaact
    14221 gctgcatgga gaaacgcttt acctggataa tccaagatcc aacaaaaaag gaagaaccgt
    14281 gcgcacagtt gataactata tcgccctgct ctgttcgctg ttgcgttttg cgtatcagtc
    14341 gggatttata tcaaccaaac catttgaagg agtaaaaaaa ttacagcgaa acagaataaa
    14401 gcctgatccg ttatctaaaa cagaattcaa tgcattaatg gaaagtgaaa aaggacagag
    14461 ccagaacttg tggaaatttg ccgtttactc aggacttcgt cacggggaac tggcagctct
    14521 ggcgtgggag gatgtggatc tcgaaaaggg aatagtgaat gtcagaagaa acctgacgat
    14581 acttgatatg ttcggtcccc caaaaacaaa tgccgggatc cggacagtaa cactactgca
    14641 gcctgctctt gaagcactga aggagcaata caaactgacc gggcatcatc gcaaaagcga
    14701 aatcactttt tatcatcggg agtacggcag aaccgaaaag caaaaactgc attttgtttt
    14761 catgcccaga gtgtgtaacg gaaaacagaa accttattac tcggtaagca gtttgggtgc
    14821 gagatggaat gcagcagtaa aacgtgctgg tattcgccgc cgtaatccgt accatacgcg
    14881 gcatactttt gcctgctggc tgttgacggc aggagcgaac ccggcgttta tagccagcca
    14941 gatggggcat gaaactgcgc agatggtgta tgaaatttac ggtatgtgga ttgatgacat
    15001 gaacgacgaa caggtagcca tgttgaatgc gcggttatcg tagttgcaaa gtttgccccc
    15061 aatttgcccc atttagtacc agagaactga aataatgaaa gaaaatcaac aaattacaaa
    15121 gaaagaacaa tacaacctga acaaattaca aaaacgtctg cgtcgtaacg tgggcgaagc
    15181 cattgctgac ttcaatatga ttgaagaagg cgatcgcatc atggtttgcc tctccggggg
    15241 taaagacagc tataccatgc tggaaattct gcgcaatttg cagcaaagcg cgccaatcaa
    15301 tttttcgctg gtggctgtta acctcgatca aaagcaaccg ggcttcccgg aacacgttct
    15361 gcccgagtat cttgaaaagc tgggcgttga gtacaagatt gttgaagaga acacttacgg
    15421 tatcgtgaaa gagaagattc cggagggcaa aaccacttgc tcactgtgtt ctcgtcttcg
    15481 tcgcggtatc ctttatcgta ccgcaacgga actgggagcg acgaagatcg cgctgggtca
    15541 ccatcgtgac gatatcctgc aaacgttgtt cttaaatatg ttctacggcg gcaagatgaa
    15601 aggtatgcct ccgaaactga tgagcgatga tggcaaacat atcgtgattc gcccgctggc
    15661 ctactgccgc gagaaagata ttcagcgctt tgccgatgca aaagcgttcc cgattattcc
    15721 gtgcaacctg tgcggttcac agcctaacct gcaacgtcag gtgattgctg acatgttgcg
    15781 tgactgggat aaacgttatc cggggcgtat cgagacgatg ttcagcgcga tgcagaatgt
    15841 ggtgccgtcg catctgtgcg ataccaacct gttcgatttc aaaggcatta cccacggttc
    15901 tgaagtggtt aacgggggtg atctggcgtt tgatcgcgaa gagatccctc tacaaccggc
    15961 aggttggcag cctgaagaag atgaaaatca gttggatgag ttacggctga atgtggttga
    16021 agtgaaataa cccggatagc gcccgatgcg taagcgtatc gggctactct tatggaggcc
    16081 ggataagacg cggtcagcga cacatccggc aatgccgaag aagatgttta ctcttgcacc
    16141 cggcaattca acatttcatt attttaataa ccgcacccgg cacgtttttc ctttaatcct
    16201 cccgccctgt aactgtttcc atgctttatg agcaacagcc tgacgcaccg cgacatagac
    16261 atgcgccgga tgcacggcga ttttgccaat atctgcgcca tcaagcccga tatctcctgt
    16321 cagcgcacct aatacatcac ccgggcgcat tttggctttt ttcccgccat cgatacataa
    16381 cgttgccatt tcggtttcca gcggcacaat agaactatta actggcggcg tttgccagtt
    16441 aagttttatc tgcaacatgt cagaaatgat attggcccgc tgtgcttctt ccggggcaca
    16501 gaaactgatc gccagaccgc tatttcctgc acgcgctgta cgaccgatgc gatgcacatg
    16561 aacttcagga tcccacgcca gctcaaagtt caccaccagc tcaagcgatt taatatccag
    16621 acctcgcgca gcaacatcag tcgcaaccag tacacgggcg ctaccgttag caaaacgtac
    16681 cagggtctga tcgcgatcac gttgctccag atcgccgtgt aacgacaatg cactttgccc
    16741 tacttcattc agcgcgtcgc agacagcctg gcaatctttt ttggtattgc aaaacaccac
    16801 gcaagaggat ggctgatgca aacttaataa ccgttgcaac aacggaattt tgcctttgct
    16861 ggatgtctca taaaattgtt gttcaatagg tggcagagca tctgttgagt caatttcaat
    16921 cgctaaagga tcgcgttgca ctcgtccgct gattgcggcg atggcttccg gccaggttgc
    16981 cgaaaacaga agcgtctgtc gagatgctgg cgcaaaacgg atgacatcat caatggcgtc
    17041 gctaaatccc atatccagca tgcggtcggc ctcatccatc accagcgtat tcaacgcatc
    17101 cagtgatacc gtgccttttt gcagatgatc caacaaacgg cccggcgttg ccacgataat
    17161 atgcggcgca tgttgcaacg aatcacgctg cataccgaac ggttgaccac cgcacaacgt
    17221 caaaattttg gtatttggca gaaaacgcgc caaccgacgc aattcacctg ccacctgatc
    17281 cgccagttca cgcgtaggac acagcactaa agcctgggtt tgaaatagcg acgcatcaat
    17341 ttgctgtaac aagccgaggc caaaagccgc cgttttgccg ctgccggttt tcgcctgcac
    17401 gcgaacatct tttccggcaa ggatcgccgg aagcgcggcg gcctgcaccg gcgtcatagt
    17461 taaataaccc aactcattaa ggttcgtgag ttgggcggga ggcaaaacat tcagggtaga
    17521 aaaagcggtc acaatctatt ctcgtggtca tcgacgcaaa gttagcaggc gcgtatcctc
    17581 gcagatctac gctcacgatg cgacaattta atcggttctt catcgggtgg tgggtcaggc
    17641 atgggttgcg gacgagggat cggatcgggc actggaacag gatcgccagg aatcggttca
    17701 gggacaggaa tttgcaaata aataagtgtc gtcatatttc cctccggtca ttgggtggac
    17761 tcttaaaggg tagacgctga taaataacag gcaaaaaaaa gccgactcat caaagtcggc
    17821 gtcgtacgaa tcaattgtgc tatgcagtaa ttcaaaaaag gaagtaagac aatatggagc
    17881 gcaacgccca tcgcttgacg ttgcattcac ctgcaagaga gatattgccc tgaatgggta
    17941 gagagtttat tgacttcgct caaactttgc ggcgtttttg tatacagaca tccgggaaaa
    18001 ttgcttttgt tacaaccatt tactacgatg caaccataaa gcaacaccac caataagaac
    18061 aactaacaga atacaaaaaa ttgaaaatcc gaattgccac ccgccgcctg ggatcccacc
    18121 aaggttgacg ccaaataacc cggtcagaaa ggtactgggt aaaaagacca ttgccatcaa
    18181 cgacattgta taggtacgac gcgctaaatt ttcctgcatc acctgagcga tttcatccgc
    18241 catcacgcca gtccgtgcta tacaggcgtc gatttcgtca agcccgcgcc caaggcgatc
    18301 ggcaatatcc tgcatacgac ggcgttggtc atcgctcatc cacggcaaac gttcactggc
    18361 aagacgagca taaacatcac gttgcggtgc catatagcga cgcatcacaa ttaattgttt
    18421 gcgcagcaga gccaggaatc cacgcggtgg aatttgctga tcaaggagat tatcttcaag
    18481 gtcgataatt ttatcgtgca gctgctcgat aaattcgctg gaatgatcgg tcaacgcatc
    18541 acatacatcc accagccatc ccccgcaatc ggtcggaccc gtgccctctt ccagatcgct
    18601 taccacatcg tccagcgcca gcactttgcg ttgtcgggtc gaaacaatta accgcccgtc
    18661 catatataca cgcatggcga ccagttgatc ggggcgttca tcggtgctgc cgtttataca
    18721 gcgcaatgta atcagcgtgc cttcaccgag acggctgact cggggccgtg tgctctcgcc
    18781 cgccagcgca tcacgtacgt tattgggaag cagcggtgtt gtcgccagcc attgggcgct
    18841 atcatggtgt acataattaa ggtggagcca gcagggatgc gcttcatcaa tcacatctgt
    18901 attttccagc ggtttaacgc cgcctctacc atccagcatc caggcaaata ctgcatccgg
    18961 gacattaacg tccgatccct taatcgcttc cacagtgcct ccatcatcaa cgcattattt
    19021 tgtagtctag ccttctggcc ctgttacgca acatctcatc accccattac cctgaaatga
    19081 ttaataaaat tctgtctaaa ttgaatacaa aaagcaaaat gcttttccat atacaaaccg
    19141 tgtgaagtgt taaatagcgt ctatcattat cagaattatc tgatcatatg acgtggcttt
    19201 tttgcgatcg gatagcaaca aaaattgata aaaataacgg gatctcaatg attacgcaca
    19261 acttcaatac cctggactta ctcaccagtc ctgtctggat cgtttcgccc tttgaggaac
    19321 agttaattta tgccaacagc gcggcgcgac tgttgatgca agacctcacg tttagtcagc
    19381 tacgaaccgg tccctattcc gtctcctcac aaaaagaact gccgaaatac ctctccgatc
    19441 tgcaaaacca acacgatatt atcgaaatcc tcaccgttca gcgcaatgaa gaggaaacag
    19501 cattgagttg tcggcttgtt ttgcgaaagc tgacagaagc agaaccggtg attattttcg
    19561 aaggtatcga agcaccggca acgctgggtt taaaagccag tcgctcggca aattatcagc
    19621 gcaaaaaaca aggtttttat gcgcgctttt ttctgactaa ctctgcacca atgttgttga
    19681 ttgacccctc acgagatggc caaatcgttg atgctaacct cgccgcgctc aatttctatg
    19741 gctataacca tgaaacgatg tgccagaaac atacctggga aataaatatg ctcgggcgac
    19801 gcgtcatgcc tatcatgcat gaaatttcgc atttacccgg tggccataag cctttgaatt
    19861 ttgttcataa actggcggat ggttcgactc gtcatgtgca gacctatgcc ggtccgattg
    19921 aaatttatgg cgacaagctc atgttatgta ttgtgcatga tattactgag caaaaacggc
    19981 tggaggagca gctggaacat gctgcccacc atgacgcgat gaccggatta ctgaatcggc
    20041 gacagtttta tcacattacc gagccaggcc aaatgcagca cctcgccatc gctcaggatt
    20101 acagcttatt gctcatcgac accgatcgtt ttaaacacat taacgatctc tatgggcatt
    20161 ctaaaggtga tgaggtgtta tgcaccctcg cccgcactct cgaaagttgc gctcgcaaag
    20221 gcgatttggt gtttcgttgg ggaggcgaag agtttgtctt attgctacca agaaccccac
    20281 tggataccgc gctttcgctg gctgaaacta tccgcgtaag cgtggcaaaa gtgagtattt
    20341 cgggcttacc acgctttacc gtcagcattg gtgtggcgca tcacgaagga aatgaaagca
    20401 tcgatgaact gtttaaacgc gttgatgatg ctttgtatcg ggcgaaaaat gatggacgca
    20461 accgcgtgct ggcggcataa gccgcggatg cgtctcgaga tcaacgactg cgcttagcgt
    20521 ggcgctccca gttttcttgc tttgcctgtg ccgttttacg tagtgcgacg taacacgccc
    20581 cgctgccgcc atgatgcggt agcgcggtac aatatgcctg gacatcatca aattcggtca
    20641 accagcgcgc cacatagctg cggacaatat tggcatgcga tttatcttcc cgccctttac
    20701 catgaataat cagcacatta cgcaaaccat ccgccagggc ttgttgaatg aaactgaaca
    20761 ccattttgcg gcactcttcc accggctggc gcaaaagatt caggctcgcc tgttgcggat
    20821 atttaccact gcgcagctta tccagcaccc catgttgcaa cccttcccgc cgaaactcca
    20881 gcggctgact tagcgggatg atgtcgagaa atccggtggt gaggaaatta tcaagttgca
    20941 gcgtgtcgat acgctgcggc gcacgttggt tacgcgttgg atgccagtgg acatctgtag
    21001 cacgtttcag cggctggaca tcttccatgg cgtcaagaaa cagcgatttg tcgtcaaggt
    21061 tcatgtaacc tcctatgcac cttcacacgt cgtgattcag acgggttaat tgttcatact
    21121 tactgcatcg ttcccttcac agattgaaac aatactctac ttcatacctg tgagtatctg
    21181 cgcaggcggg gcattttata ctagcctgtt agcagtatcg aacagtttag cccgagttta
    21241 attcagcaca ggctccgatc aggatgattt attcatagca ggaaccaaca aaaatgcgat
    21301 aaataacaga ccactgatta gcagaaaggt atccgaaaat gccatagttt gcgcctccct
    21361 cagcatcaat tgactcaata atttggttga agccagtatt tccgatgtct ggtcacttcc
    21421 actgcgatca aaaaacaaag cacttctgga aatgaagtca ttcaccgtat ggggaacaga
    21481 aaccattttt tcacccattc tcgaaaaatg aaaattagtt cgattattga gaatagagcc
    21541 acacagggca ataccaatcg cgcccccaag attacgtgtt aaattaaata caccagaggc
    21601 taacttcagc cgttcttttg gaattcctcc cagcgttaat gttacgatgg gggccatcgc
    21661 aaactgctgc gaaatccctc taattgcctg tggaaacagt aactcctgcc atccccattc
    21721 gtgggttata ggggtaaaaa gatacattga aaacacaaat ccacccagcc ccgccatcaa
    21781 cagccatcgc agatttattt ttttacttaa ccagaagtaa aagggaacag aaaataactg
    21841 gaaaatacct gttgtgcaaa cggcaaaacc tatctcttcg gcatttaatc cgcgaacctg
    21901 ccccaaaaac acaggtatta aatatacagt agagaatatc ccaacccctc cagaaaaaga
    21961 aaaaaaacat cccagcgtga aatttttatc cttaaaagca tgaagatcca ttatcggatt
    22021 ggatattgta agtgtacgcg cagcaaataa aataaaactc accaatgcca gaactgatgt
    22081 cagcaaaata gtattatcat ccagccatcc ccagcgcgct ccttcttcaa gagtatattc
    22141 cagacatccc agtgtcatgg ccaacaggat aatactcgga taatcagcaa ctttcagtaa
    22201 agagagatca ggtttatcaa aattaaccag aaaaggaata ctcaaaacta aatatatgcc
    22261 aggtaatata ttgatataaa atagccatcg ccagtcgagg ttatcagtga tccagccccc
    22321 cagagttggc cctagcgtcg gtgacagtga ggccagcgca ctgactaccg cagcagccaa
    22381 ccccaactct ttaccctggt aataaataaa cgccgtcgtg aaaactaaag ggatcatgga
    22441 tgcgccagcc gctccctgta aagcacgaaa caaaatcatg atctgaatat tccatgcaag
    22501 tccacacgcg atactcatta aagtaaatat tcccgcggat agagtaaaaa gccaacgagt
    22561 ggaaaatacc cgtgacagcc acccagagag cggaataaca ataatctcag caattaaata
    22621 gctggtctga agccaggcca tttcgtcttt acctgcggac agaccaccac ctatttcatt
    22681 tagggatgac gatacaattt gaatatcaag ctgagcaata aataatccca tacacatact
    22741 ggcgaaagca atatgtttac gcatagacat catctctcat caacactgac ggtgacggac
    22801 agtcccgggc gaagaagttg ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg
    22861 acacgctgaa caattttggt gaaattgcca gtggcatttt ctatcggaat caagctgaaa
    22921 ctggctcctg tcgctgggga aaggctttca atgtgaccgt gaaacacttc ccctttgaga
    22981 atatcagcac ggatctcagc cttcattcct ggcttcattc ccgctatttg gttttcttta
    23041 taatttgcat caacccacaa ctcactgact ggtacaagtg aaactaacga tgtgccgcct
    23101 tccacccaac tgcctgtatg ggcgctgcgg tttgcgacaa tgccatcaat cggcgcacga
    23161 acaagggtat attccagatc cagtagtgcc tgagtcaaag cggcctgaac ttcttcgata
    23221 ctcgcacgta cgttttcttc ctgagcagat aaaactgcca gccgttgctt ctccaccagg
    23281 aaatcgttcg cggctttgcg ctcctttgct gcgacctgct ggtagtcaaa cctggcgtta
    23341 tcaataattt gttgcgatat tgccgctgat tgtgcgagtt tttcataacg ttcagtatct
    23401 cgcaggcgct tttgctcctc atgttttact gcctgccatg tttcactggc actttgaatt
    23461 atcgactgct gcatcgcaat agtggcttga atgctttcga gattggcttt gctacttttt
    23521 atttttgcct caagcctggc gacatttgca cgataatccc gatcatcgag acgcaggata
    23581 atatcgcctt tttttacgct ctgattatcg cgcacctcta tagcagaaat ataaccagaa
    23641 actttacttg ccaccgtggt gatattacca ccaatgtaag catcatccgt cgtctgtata
    23701 aatcttcctg tgcttaccca ccagataaaa aagacaacgc cagctaaaat aataccgatc
    23761 gctaccaccc caataagctg ttttttagaa attatcatca ctctttacac cgtatatatc
    23821 cggaaacttg cattcgtcat atttaaaatt taacatggct tatttgatga tttctgacaa
    23881 aatacctact gtaaatgaca atcacactgc aacccaaagc atcaccaggg catcacatta
    23941 tcggactgga ttcggaacat ctgaatggag gtacagttcc ctggcataaa catctctatg
    24001 cccagttgct ttacccggct gaaggagtcg ttcgtgtatg ggctggagaa agcgtctggc
    24061 tagtgcatgc cagcagcgcg ctttggctgc ccccacaaat gccgcataag tttgttgcga
    24121 caggcaacgt acttttaaaa accgtactgg tttcagaagc ggaaagtgaa acgctgggaa
    24181 aagtttgctt catgacgggt atttcacccc tgttaaggga attactaatt gcaattaacc
    24241 aacttccacc gtcgcaaagt accacagata aacaacagtt acgtttttca gccctcgaga
    24301 cactgatact tcaggaaata aaaatggggg tgaaaatgtc acttgaactc ccttggccaa
    24361 atgatgagcg gctacagcag ctttgtgaga atttgctaaa caaccaaggg tatctgccaa
    24421 cattggataa tctagcggat aaaattaacg tcagtagccg tacactcatg cgcttatttg
    24481 ttaaagagac gggattaaca tttcgccact gggttcagca aatgcacgtt atttctgcag
    24541 tgacgcttct tgatgatgga tattcgttga ccaaaattgc ccatcgtctg ggctatgcct
    24601 cagctgaatc gttcggcaat atgtttaagc gtcgaacggg ctactcgccc ggtaaattta
    24661 ctcgtcgatt aacgatgcat gattatgcaa tcacaagaca aatgatttaa cttatatttt
    24721 cgtgaaatct gtcactgaag aaaattggca acggatgggt aaaaccgtta taacactgtc
    24781 accggtcacc aggaccccag gccggattta agacgaggat gcactgctgt gtgtactgta
    24841 gagtctggcg gatgtcgaca gactctattt ttttatgcag ttttaacttt gcagatagcc
    24901 gcattctcgc cgaaattgcg tgattaatga cgcggtcagt ggtgtctggc gactatcacg
    24961 tcgctgaatc aaataatagg tcgctttcgg taaaatttcg ctaaccggca acatcaccag
    25021 cccctgtccg tgcaagggat cgcagcccat ttcttcaggt agtatgctga gaaaatcgct
    25081 ttttgccacc agactgatac aggctgagaa cgtctcgcag actacgccga cctgtggcgt
    25141 ttgcgcctga tcgtcaagca attcactcaa gtgtttgtag tagctgccgt gtggcgtcgg
    25201 cattgtccag ctgtagtcca gtaactgttt gatcgaacgg gcaccaatgg cgggatgtcc
    25261 cgggcggcaa aagatcgcga attgcttttc cagtaatttc tcaaaagtaa attcgtggtc
    25321 gtagggtccc tgataatagg tattgatggt gaaatccagt tccccctggc gcaattcatt
    25381 aatcatcgat accagttgcc cttccataat gcgtactttt acctgcggat gctgctgatg
    25441 aaaacgagat atgacagctg gcatcagact gcgggaaata ctggccccca tgccgatatt
    25501 aatttgccct gctagttgcc cttgtcgttg gcgaatatcc tcttgcgctg cgcgcagctc
    25561 ttcaagaata agactggcgt gctgataaaa actttcgccg gcatcagtta acgtcacgcc
    25621 tttactacgg cgaaaaaaga gttgtgccgc caacccttct tccagctcct gaatagattt
    25681 actcagtgcc ggttgcgaca tattcaacat tcggctcgct ccgcgaatgc tgccctgacg
    25741 agccacttca acaaaagccc gaatttgatg aatttttacc tgaaaagcca tgacgccacc
    25801 gataaccgtt atttatcaga ccaaagaaac tggcatctac tttaatgcag atgattgtgt
    25861 cagggtaatt tatgaacggt taaaactgtg aaaaatcagt tagtgataag taaaaactat
    25921 cgctacgtga accgggtcac actttttact gatgacggga aaggttatgg agtctttgaa
    25981 tcaatttatt aattcgcttg ccccaaaatt atcgcactgg cgacgtgatt ttcaccacta
    26041 tgcagagtct ggctgggtgg aattccgcac tgccaccctt gttgcggaag aattgcacca
    26101 gctcggttat tcactggcgc tgggccgcga agtagttaat gaaagtagcc ggatgggatt
    26161 acctgatgaa ttcactctac aacgcgaatt cgagcgcgct cgtcaacagg gtgcgctaga
    26221 acaatggatt gcggcttttg aaggcggttt cactggcatc gtcgctaccc tggataccgg
    26281 tcgccccggt ccggtgatgg ctttccgtgt cgatatggac gcgctggatc tcagtgaaga
    26341 acgggatgtc agccatcgcc cctaccgcga cggttttgcg tcatgtaacg ccggaatgat
    26401 gcatgcctgt ggtcatgatg gacataccgc cattggactt gggctggcgc atacccttaa
    26461 acagttcgag tccggactac atggcgtcat caaactgatt tttcaacctg cagaagaagg
    26521 tacgcgtggc gcgcgggcga tggtcgatgc aggtgtagtg gatgatgttg attattttac
    26581 tgccgtgcac attggcactg gcgtacctgc gggcaccgtg gtgtgcggca gtgataattt
    26641 tatggcaacc accaaatttg acgcgcactt caccgggacc gccgctcacg caggcgcaaa
    26701 accagaagac ggtcacaatg ccttgttggc ggcagcacaa gcaactcttg cactgcatgc
    26761 aatcgccccg cacagcgaag gcgcttccag agtaaacgtg ggcgttatgc aggcaggaag
    26821 cggtcgtaat gttgttcccg cctcagcgtt gctgaaagtg gaaacacgcg gggccagcga
    26881 cgtcattaat caatatgttt ttgaacgtgc acaacaagcg attcagggcg cagcaaccat
    26941 gtatggtgtc ggcgttgaaa ctcgtctgat gggtgcagct accgccagtt ctccttcgcc
    27001 gcaatgggtc gcatggttgc aaagtcaggc agctcaggtc ccgggggtca atcaagccat
    27061 tgaacgtgtt gaagcgcctg cgggttccga agatgccaca ttaatgatgg cccgcgtgca
    27121 gcaacatcaa gggcaagcct cctacgtggt gtttggcaca cagctggcgg caggtcatca
    27181 caacgaaaaa ttcgattttg acgagcaggt tctcgctatt gccgtcgaaa cgctggcgcg
    27241 caccgcgctc aattttccct ggacgcgagg tatctgatgc aggaaatcta tcgttttatc
    27301 gacgatgcga ttgaagccga tcgccaacgt tataccgata ttgccgatca aatctgggat
    27361 catccagaaa cacgttttga agagttctgg tcagcggagc atctggcttc ggcgctggag
    27421 tctgcaggct tcaccgttac ccgcaacgta ggcaatatcc caaatgcctt tattgcttcg
    27481 tttggtcaag gcaaaccggt tatcgccctg ctgggggaat atgacgccct ggcaggttta
    27541 agtcagcaag caagttgcgc gcaacctaca tccgcgacgc ccggtgaaaa tggtcacggt
    27601 tgcggacaca atttgctggg aaccgccgcc tttgccgctg caatagccgt caagaaatgg
    27661 ctggaacaat atgggcaagg cggcacggtg cgcttttatg gttgtcctgg cgaagaaggc
    27721 ggctcgggta aaacgttcat ggtccgcgag ggggtatttg atgatgtgga tgcggcactc
    27781 acctggcacc cggaagcctt tgccggtatg ttcaataccc gcacgctggc aaacattcag
    27841 gcatcatggc gctttaaagg gatcgcagca catgccgcga attcccctca tttgggacgc
    27901 agcgcccttg atgccgtaac gttgatgacc actggcacca acttcctcaa cgaacatatt
    27961 attgaaaaag cgcgcgtaca ctatgccatc acagatagcg gcgggatctc gcccaacgtg
    28021 gtccaggcgc aggcagaagt gctttatctt atccgcgccc ccgaaatgac cgatgtgcag
    28081 catatttatg atcgggtcgc caaaatcgcc gaaggtgcgg cattgatgac cgaaaccacg
    28141 gttgaatgcc gcttcgacaa agcctgttcc agttatctcc cgaatcgcac cttagaaaat
    28201 gccatgtacc gagccctatc ccattttggt accccggaat ggaactgcga agaactggct
    28261 tttgcgaaac aaattcaggc tacgctcacc cccaacgatc ggcaaaacag tctgaataat
    28321 atcgctgcaa ccggtggcga aaacggcaag gcttttgcac tacgtcatcg tgaaacggta
    28381 ctggcgaatg aagtcgctcc atatgccgcc accgataacg tgcttgcggc atcgactgat
    28441 gtcggcgacg tcagttggaa actgcctgtt gcccagtgtt tcagcccctg ctttgccgtt
    28501 ggtaccccgc tacatacgtg gcaactggtt agccaggggc gaacatctat tgctcataaa
    28561 ggaatgctgc tggcggcgaa aactatggca gcaaccacac ttaatctctt cattgattca
    28621 gggctattgc aagaatgcca acaagagcat cagcaagtta cggacacgca accgtatcac
    28681 tgccctatcc cgaaaaacgt gacaccgtca cctttaaaat aacaacaaca acgcaaacac
    28741 aacaaacgag gaatgcccat gagtatgtca tccataccgt cgtcctccca atccgggaag
    28801 ctctatggct gggtcgaaag aattggtaac aaggttcccc atcctttttt gctctttatc
    28861 tatttgatta tcgtactcat ggtgacgacg gcaattttgt cggcctttgg cgtcagtgca
    28921 aaaaacccga ccgatggtac gccggtggtg gtgaaaaacc tgctcagtgt ggaaggatta
    28981 cactggtttt tacccaatgt tattaaaaac tttagcggtt ttgctccact tggtgcgatc
    29041 ctggcgctgg ttttaggtgc cggtctggcg gagcgcgtcg gcttactacc agcgctaatg
    29101 gttaaaatgg catcgcatgt taatgcccgc tacgccagtt atatggtgct gtttattgct
    29161 tttttcagcc acatttcttc cgatgcggcg ttagtgatca tgccaccgat gggtgcgcta
    29221 atttttctgg cggtgggcag gcatccagtt gcaggtttac ttgccgccat tgcaggcgta
    29281 ggttgcggct ttacggctaa tttactgatt gtcacaaccg acgtgttgct gtcggggatc
    29341 agcacggagg ctgcggctgc gttcaatccg caaatgcacg tcagtgtaat tgataactgg
    29401 tattttatgg ccagctccgt agtcgtactg acgattgttg gcggcctgat aaccgacaaa
    29461 atcattgagc cacgtttagg tcaatggcag ggaaacagcg atgagaaact gcagacattg
    29521 accgaaagtc agcgttttgg tttacgcata gcaggtgtcg tatcgctact ttttattgct
    29581 gcgattgcgc tgatggtgat cccggaaaac gggatattgc gcgatccgat taatcacacc
    29641 gtgatgccat ccccctttat taaaggtatc gtgccactga tcattctttt tttctttgtg
    29701 gtctcgctgg cttatggcat cgctacccgc acaattcgac gtcaggcgga tttaccgcat
    29761 ttaatgattg aaccgatgaa agagatggcg ggatttatcg tgatggtttt tcccctcgcc
    29821 cagtttgtcg ccatgtttaa ctggagcaac atggggaaat tcatcgccgt ggggctgacc
    29881 gatatactgg aaagttcagg gcttagcggc atcccggcgt ttgtcggtct ggcgttgctt
    29941 tcctctttct tatgcatgtt tatcgccagc ggttccgcaa tctggtcgat tctggccccc
    30001 attttcgtac cgatgtttat gctacttggc tttcacccgg catttgcgca aatcctcttt
    30061 cgtattgccg actcatccgt attgccttta gcgccagtat ctccttttgt tccactgttt
    30121 cttggattcc tgcaacgcta caaaccagac gcgaaactgg gtacttacta ttcgttagtt
    30181 ttgccctatc cacttatctt tttggtggta tggctgctga tgttgctggc gtggtatctt
    30241 gtcggtctgc cgataggtcc ggggatttac ccacgtttgt cttaagagag aacggatgct
    30301 gagattactt gaagaaaaaa ttgtcacgcc actgggtcca ctgtgggtga tttgcgatga
    30361 gcaatttcgc ctgcgggcgg ttgaatggga agagtacagc gaacgcatgg tgcagctgct
    30421 ggacatccat tatcgcaaag aaggctatga gcgcatttct gccaccaacc caggcggttt
    30481 aagcgacaaa cttcgtgatt attttgccgg taatcttagc attattgata cgcttcccac
    30541 tgccacaggg gggacgccat ttcagcgcga agtctggaaa acactacgca ctatcccctg
    30601 cgggcaggta atgcattacg gtcaactggc cgaacaattg ggtcgccctg gcgcggcgcg
    30661 tgccgttggt gcggctaacg gatcgaatcc catcagcatt gttgtgcctt gtcatcgggt
    30721 cattggcaga aacggtacca tgaccggata tgcaggcgga gttcagcgaa aagagtggtt
    30781 attgcgccac gaaggttatc ttttgctgta aacattaaac aatttgtgcc agcttgttca
    30841 cacttttatg taaagttacc cttaacaact taagggtttt caaatagata gacatatatt
    30901 tacatctaat atcggaattc tctgctgtta aggtttgctt agacttactt gctccctaaa
    30961 aagatgttaa aattgacaaa tatcaattac ggcttgagca gacctatgat cccggaaaag
    31021 cgaattatac ggcgcattca gtctggcggt tgtgctatcc attgccagga ttgcagcatc
    31081 agccagcttt gcatcccgtt cacactcaac gaacatgagc ttgatcagct tgataatatc
    31141 attgagcgga agaagcctat tcagaaaggc cagacgctgt ttaaggctgg tgatgaactt
    31201 aaatcgcttt atgccatccg ctccggtacg attaaaagtt ataccatcac tgagcaaggc
    31261 gacgagcaaa ttactggttt ccatttagca ggcgatctgg tgggatttga tgccatcggc
    31321 agcggtcatc acccgagctt cgcgcaggcg ctggaaacct cgatggtatg tgaaatcccg
    31381 ttcgaaacgc tggacgattt gtctggtaaa atgccgaatc tgcgtcagca gatgatgcgt
    31441 ctgatgagcg gtgaaatcaa aggcgatcag gacatgatcc tgctgttgtc gaagaaaaat
    31501 gccgaggaac gtctggctgc attcatctac aacctgtccc gtcgttttgc ccaacgcggc
    31561 ttctcccctc gtgaattccg cctgacgatg actcgtggcg atatcggtaa ctatctgggc
    31621 ctgacggtag aaaccatcag ccgtctgctg ggtcgcttcc agaaaagcgg tatgctggca
    31681 gtcaaaggta aatacatcac catcgaaaat aacgatgcgc tggcccagct tgctggtcat
    31741 acgcgtaacg ttgcctgatt tttccgcata actcactatc cttctgtcat atcattaaat
    31801 ttttctgatt tattgatctg gcagaaggtt catcactgtt tcattcacca gatatgggtt
    31861 aatcttttaa ttacaaactg cgttgacagt tgttgtaagg agaccctgta tggctatgta
    31921 tcagaacatg ctcgttgtta tcgatcctaa ccaggacgac caaccagcat tgcggcgagc
    31981 tgtttattta catcaacgga ttggtggcaa aattaaagcc tttttgccga tctatgactt
    32041 ctcatacgaa atgaccaccc tgctctctcc ggacgaacgt accgctatgc gtcagggcgt
    32101 catcagccag cgtacagcct ggatccacga gcaggcaaaa tattatctca atgctggcgt
    32161 tcccattgaa attaaagtgg tctggcataa ccgtcctttc gaagccatca ttcaggaagt
    32221 gatcagcggc gggcacgatt tggtgctaaa aatggcgcac caacatgacc gtctggaagc
    32281 ggtgattttt acgccaacgg actggcatct gttacgcaaa tgcccaagcc cggtgtggat
    32341 ggtgaaagac cagccgtggc cggaaggggg taaggcgctg gtggcggtga atctcgccag
    32401 tgaagagccg taccataatg cgctcaatga aaaactggtc aaagagacga tcgaactggc
    32461 agaacaagtc aaccatactg aagttcatct ggttggcgct tatccggtaa cgccaattaa
    32521 tatcgcgatt gaactgccgg aatttgatcc gagcgtttat aacgatgcca ttcgtgggca
    32581 acatttgctg gcaatgaaag ccctacggca gaaattcggc attaatgaaa acatgacgca
    32641 cgtagaaaaa ggtctgccag aagaggtgat tcctgatttg gcggagcatt tacaggcggg
    32701 gattgtggtt ctgggcacgg tcggacgcac cggtatttca gcagcattcc tcggcaacac
    32761 ggcggaacag gtgattgatc atcttcgctg cgacctgctg gtcattaaac ctgaccagta
    32821 tcagacaccc gttgaactgg atgacgaaga agacgattaa cgactcttca ataactgaca
    32881 aggcccgagt tatcgggcct attttttact tactgctctt cgccgccaag aaaataaatt
    32941 cccaacggta ttgccagcaa cacagtaaag accaggctat aaacaaaaat catcgatgac
    33001 tgaatcacat acattgatgt cgtccactca gacagcggaa tattgtactg ttctataacg
    33061 ccgccgatgg tcgcgcgagt cacaaccgac gccgcaataa tgaaaaccgc caacaaacca
    33121 aagagaaatt tcttcccttt cgctgacttc agtttcgcca taatcatgat gtaatccttg
    33181 tgcagacaaa tttcttgtta cctaaacgta acaataccat gacattttac cgttgtctgc
    33241 tctgaaaacc ttacctctta gctggattta caatcaacgc attgattagt tagctaatgt
    33301 tgctacttcc ttccctgact attgatgatc tgaatgaatt gtcgcggtaa aatagccccg
    33361 ccaggggttt ccggcatgac gacaattatc tggaggagcc tgataaaaca atgatcgctg
    33421 aactgtttac aaataatgcg cttaatctgg tcattatttt cggtagctgc gcagcattga
    33481 ttctgatgag cttttggttt cgccgcggaa atcgtaaaag aaaaggtttt ttattccatg
    33541 cggtgcaatt tttaatctac accataatta tcagtgctgt tggtagcatc attaattatg
    33601 ttatagaaaa ctataaactc aaatttatca ccccaggcgt tatcgatttt atctgtacgt
    33661 ccctgattgc ggttattctg acgattaagc tatttctgct gattaatcag tttgaaaaac
    33721 agcagattaa aaaaggtcgc gatatcacca gtgcgcggat tatgtcgcgt atcatcaaaa
    33781 tcaccattat tgtggtgctt gttctgcttt atggcgaaca tttcggcatg agcctttctg
    33841 gcttgttgac ctttggtggt attggtggtc tggctgtcgg tatggccggt aaagatattc
    33901 tgagtaactt cttttccggg attatgctct atttcgaccg tccttttagt attggcgact
    33961 ggatccgttc accggacaga aatatcgaag gtacagtagc ggaaattggc tggcgaatta
    34021 ccaaaattac gacctttgat aatcgtccat tgtacgtacc gaactcgctg ttttcgtcga
    34081 tcagcgtaga aaatcctgga cgaatgacca accggcgcat taccacgacc attggtttac
    34141 gttatgagga tgcggcaaaa gtgggcgtta ttgtcgaagc cgtacgtgag atgctgaaaa
    34201 atcacccggc catcgaccag cgacaaacct tactggttta tttcaaccag tttgccgatt
    34261 cgtcattaaa tattatggtt tattgcttta ccaaaaccac ggtatgggct gaatggcttg
    34321 ctgcacagca agacgtttat ttgaagatta tcgatattgt acagtcacac ggcgcggact
    34381 ttgccttccc cagccagacg ctgtatatgg ataacattac accgccggac cagggtcgct
    34441 aaatcagaca tcactggcgt tatccacaag ggtaacgcca attcccatca catcaatgct
    34501 tcacaatata catagtccga ctgtacgcca catcttccgg gttattaatg ggataacctt
    34561 tcagccacgg cttgattaat cgtccattgg tatattgata aattggtgca atcggtgctt
    34621 gctccatgag gattttttct gccgcattgt aatcggcatt acgcgcttta acggtatttt
    34681 ccgttgatgc ctgggccagt actttgtcgt atgccggatt gttaaagcgt gaaatattcc
    34741 ctgaatgcgt tgacgttaac aacgtaagga aagtggaggg ttcattataa tcccctaccc
    34801 atgaggcgcg gataacatca aaattgccgg tgttacggct atcgatatag gttttccatt
    34861 cctgattctg caatttaaca tctacgccaa ggttcttttt ccacatcgat gctacagcaa
    34921 tcgcaatttt ttgatggttt tctgaagtgt tatacaaaag cgtcagcttc agcggttttt
    34981 gcggaccata accagcggcg ctcaacaaag tttttgcctg cgcattcagt tcttcctgac
    35041 tcatttgttc aaacggcgaa ggctccggcg taaaaccggc agtaacatct ggtgtaaaat
    35101 gccacgctgg cttttccccc gtccccaaca ctttttcggt catcaggcgg cgatctatcg
    35161 tcatacttaa tgccaggcga acgcgctgat cggccgtcgg ccctttttgc gtgttaaacg
    35221 cataataata ggtcccgagc tgcggcggcg tataaacctg ccccggaata tccttcaaca
    35281 gcttctgata catatttttc gggaaggatt cggtgatatc aatatccccc gcgaggtaac
    35341 gcttagtggc tgcggattcc tgattaattg gcaggaaggt cactttttgc agtaccgttt
    35401 tggcgttatc ccaataatgg gtattcggta ccacgaccag tttttcattg actacgcgct
    35461 ctttaagaac ataagcgcca ttgccgatca gttttccggg tttcgtccac tctttactgc
    35521 tttctacgtt ggctttttgc accgggaaga aggcaaagtt agcggttaaa ttcacaaacc
    35581 acggcaacgg tttatcaagc tgaattttca aagtatgggc atcaactgcg gtgacgccaa
    35641 gctggtcagg cgtagcttta ccatcaataa tcgcctgtgc gttgttgatt cccgccagcg
    35701 cggcaaacca tgcaaatggc gatagcgttt tcgggtccac cagacgctgc cagctgtaga
    35761 caaaatcttg cgccgttacc ggtgtgccat ccgcccattt tgcgttatcg cgcagggtaa
    35821 aagtccagat acggttgtca ttacttttcc actgagtcgc aacgccgggg acaatctccc
    35881 ctttctcgtt ctgattcacc agtccttcaa acagatcgcg aatgacctga atctctggca
    35941 ggcccacggc tttagcggga tccagcgacg caggctcatc tttaatgtgg cgcaccaact
    36001 cctgcttctc tgccagtact gtgccgctcg gaacttctgc agcatacgaa agagaaatgc
    36061 tgctgatcaa cagcgcacaa cacgtgactg aaacagagtg cttcataagt ttccctctaa
    36121 ccgacattaa aattgatgcg taattatttg tttcgcctta aagaaatgca aacaacttcc
    36181 atcaaaaaag tgacaaagga agcatcaaat gttttaagta tcaatatatc aaatcagatt
    36241 tagtgtaaat aatagtctcc aaaatgcggt aaagcacata gaactccgaa cactacgttc
    36301 gggcttcgca cgttcaaacc cgttttcggt atcataatca gcatctgtcg ccattgtgcg
    36361 aagaataaaa aataaccaaa acgttgaaag gaactgagaa tacgttaaga gaagaattaa
    36421 attaatgtat cagcaagtag tgctaaacag cacctttata cttcagccta tctataaata
    36481 atgagaatgc cgagccagca tgtcgacggt gtggatagta caggtgataa ccgggtaaat
    36541 ctggtgtgaa tttatccaaa acacgtatca gttttttttc tttaatagcc cgctcaacct
    36601 gatcataagg tagatacgcc aatccatgcc catcaattgc agcatcaatg atcaggtcta
    36661 tcgtattcag taaaagctga ccttccatgc gaacacgaac ttcacgtcca ccgcgtatta
    36721 atctccagcg atttgctgta cccgatgtgg gaagatacaa attaattgcc tgatgatcta
    36781 ttaattgtga cactgacgtt ggagcacttc gacgagaaaa ataatccggt gagccaacaa
    36841 tagccattgg tatatctggc ccgattcgaa tggcgatcat atctttatcc atctccccac
    36901 ccagacggac gcctgcatca aaacgttcag aaacgacatc ggtcaaacca taatcaatgg
    36961 taagctggat atcaatttca ggatgcgatt tcaggaatgt gcgcatagct ggtaacaata
    37021 tcgtttttgc tgcatgttct acagtagtaa tacgtattgt cccggatggg cggttctgca
    37081 gatcgctcag ggatgtcagg gcagaatcta tgtcatgcaa catagggcca agaacagaca
    37141 aaagatgctc gcccgcttca gtgggtacaa cgctgcgcgt ggttcgcgtc agaagccgca
    37201 atcctaatcg ttcttctata cggcgcacta tctggcttaa agctgattgc gccatgctca
    37261 ggcgggctgc tgcgcgagtg aagctacgct cctctgcaac gacgacaaac gccatcagat
    37321 cagcgatttc ttcacgcttc attcagtatc tccatacaat ctattaatca tttaaatgat
    37381 aggtctaatc ataatttagg gtctaatcaa cttaatgatc aatatctata gtgatatcac
    37441 caaccgccaa aacgaaccga caactccatg aggaacggaa tgatgaataa taaagtcagc
    37501 ttcactaaca gcaataatcc aaccatctct ttgtctgcag tgatctattt ccctccaaaa
    37561 tttgatgaaa cccgtcagta tccggcaatt gtggtatctc acccaggtgg gggcgttaaa
    37621 gagcaaacgg ccggaaccta tgccgaaaaa ctggcggaaa agggatttgt cactattgct
    37681 tatgacgcat cttatcaagg tgaaagcggc ggcgagccgc gtcagttaga aaacccatac
    37741 attcgtactg aagacgtcag tgccgtgatt gattacctga caacgctttc ttatgtcgac
    37801 aatacccgaa ttggtgcgat gggaatttgt gccggtgcag gatatacagc caatgctgca
    37861 attcaggatc gccgcatcaa ggccatcggt accgttagtg cggtcaatat tggttcgatg
    37921 ttccgtaatg gttgggaaaa caatgtgaaa tctattgatg ccctaccata cgttgacgcg
    37981 ggttcaaacg cccgaaccag cgatattagc agcggagaat acgccaccat gccactggca
    38041 ccaatgaagg agtccgatgc gccgaacgaa gaactgcgtc aggcctggga gtattaccac
    38101 acccctcgcg cgcagtatcc aacagcaccg ggttacgcta ctttgcgcag ccttaaccag
    38161 attattacct atgatgctta ccatatggcg gaagtgtacc tgactcagcc gatacagatt
    38221 gtggcgggca gccaggcagg gagcaaatgg atgagtgacg acctgtacga tcgagcatca
    38281 agccaggata aacgctatca cattgttgaa ggcgcaaacc atatggatct ttatgacggc
    38341 aaagtctatg tggctgaagc aatttcagta ttagcgccgt tctttgagga aactctgtaa
    38401 tgaaacatat tcaaatccgt aatagtgata tggcctggca tattgctgcc aataatctgg
    38461 gaaaagtttc atgaaaaatg tactgattct tggtgctggc ggtcagatag cccgccatgt
    38521 gattaatcaa ctggcagata aacaaaccat taaacaaact ttatttgccc gtcagccagc
    38581 aaaaatccac aaaccctatc caactaatag caagattatt atgggggatg tactgaatca
    38641 tgcagcactg aagcaggcca tgcagggaca ggatgttgtg tatgccaacc ttacggggga
    38701 agatttagat attcaggcaa attcagtgat cgctgcgatg aaagcctgtg atgttaaacg
    38761 tctgatcttt gttctttccc tcggtattta tgatgaagtt ccaggaaaat ttggcgaatg
    38821 gaataatgca gttattggtg aacccctaaa accattccgc agggctgcgg atgcaattga
    38881 ggcttctgga ctggaatata caattcttcg ccctgcctgg ctgactgatg aagatattat
    38941 tgattatgag cttacctcac gcaatgaacc attcaaaggt acgatcgtat cacgtaagag
    39001 tgtcgcagca ttaattacag atatcatcga taaacctgag aaacacattg gtgagaatat
    39061 aggcatcaat caacccggga ctgatggcga taagccat
//
LOCUS       DAOTRK010000024        38697 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.024, whole genome shotgun sequence.
ACCESSION   DAOTRK010000024 DAOTRK010000000
VERSION     DAOTRK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.024"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(10..1869)
                     /gene="gss"
                     /locus_tag="QIE41_001277"
     CDS             complement(10..1869)
                     /gene="gss"
                     /locus_tag="QIE41_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDS3530186.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2074..2940
                     /gene="yghU"
                     /locus_tag="QIE41_001278"
     CDS             2074..2940
                     /gene="yghU"
                     /locus_tag="QIE41_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDS3530187.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLELGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3172..3420)
                     /gene="hybG"
                     /locus_tag="QIE41_001279"
     CDS             complement(3172..3420)
                     /gene="hybG"
                     /locus_tag="QIE41_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDS3530188.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3433..3774)
                     /gene="hypA"
                     /locus_tag="QIE41_001280"
     CDS             complement(3433..3774)
                     /gene="hypA"
                     /locus_tag="QIE41_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDS3530189.1"
                     /translation="MHELSLCQSAVEIIQRQAEQYDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3767..4255)
                     /gene="hybE"
                     /locus_tag="QIE41_001281"
     CDS             complement(3767..4255)
                     /gene="hybE"
                     /locus_tag="QIE41_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDS3530190.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4248..4742)
                     /locus_tag="QIE41_001282"
     CDS             complement(4248..4742)
                     /locus_tag="QIE41_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDS3530191.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4742..6445)
                     /gene="hybC"
                     /locus_tag="QIE41_001283"
     CDS             complement(4742..6445)
                     /gene="hybC"
                     /locus_tag="QIE41_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDS3530192.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6442..7620)
                     /gene="hybB"
                     /locus_tag="QIE41_001284"
     CDS             complement(6442..7620)
                     /gene="hybB"
                     /locus_tag="QIE41_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDS3530193.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7610..8596)
                     /gene="hybA"
                     /locus_tag="QIE41_001285"
     CDS             complement(7610..8596)
                     /gene="hybA"
                     /locus_tag="QIE41_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDS3530194.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8599..9717)
                     /gene="hybO"
                     /locus_tag="QIE41_001286"
     CDS             complement(8599..9717)
                     /gene="hybO"
                     /locus_tag="QIE41_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDS3530195.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9906..10193)
                     /locus_tag="QIE41_001287"
     CDS             complement(9906..10193)
                     /locus_tag="QIE41_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDS3530196.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10312..11199)
                     /locus_tag="QIE41_001288"
     CDS             complement(10312..11199)
                     /locus_tag="QIE41_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDS3530197.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11356..12396
                     /gene="gpr"
                     /locus_tag="QIE41_001289"
     CDS             11356..12396
                     /gene="gpr"
                     /locus_tag="QIE41_001289"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDS3530198.1"
                     /translation="MVWLANPERYRQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRALLRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12436..12930)
                     /locus_tag="QIE41_001290"
     CDS             complement(12436..12930)
                     /locus_tag="QIE41_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDS3530199.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13121..14005
                     /gene="yghA"
                     /locus_tag="QIE41_001291"
     CDS             13121..14005
                     /gene="yghA"
                     /locus_tag="QIE41_001291"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDS3530200.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14044..14469)
                     /gene="exbD"
                     /locus_tag="QIE41_001292"
     CDS             complement(14044..14469)
                     /gene="exbD"
                     /locus_tag="QIE41_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDS3530201.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14476..15210)
                     /gene="exbB"
                     /locus_tag="QIE41_001293"
     CDS             complement(14476..15210)
                     /gene="exbB"
                     /locus_tag="QIE41_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDS3530202.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15462..16649
                     /gene="metC"
                     /locus_tag="QIE41_001294"
     CDS             15462..16649
                     /gene="metC"
                     /locus_tag="QIE41_001294"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDS3530203.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHVQPNTKIVFLESPGSIT
                     MEIHDVPAIVAAVRNVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16789..17448
                     /locus_tag="QIE41_001295"
     CDS             16789..17448
                     /locus_tag="QIE41_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDS3530204.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTILMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17488..18387)
                     /gene="yqhC"
                     /locus_tag="QIE41_001296"
     CDS             complement(17488..18387)
                     /gene="yqhC"
                     /locus_tag="QIE41_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDS3530205.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18581..19744
                     /gene="yqhD"
                     /locus_tag="QIE41_001297"
     CDS             18581..19744
                     /gene="yqhD"
                     /locus_tag="QIE41_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDS3530206.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19849..20676
                     /gene="dkgA"
                     /locus_tag="QIE41_001298"
     CDS             19849..20676
                     /gene="dkgA"
                     /locus_tag="QIE41_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDS3530207.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20876..21802
                     /locus_tag="QIE41_001299"
     CDS             20876..21802
                     /locus_tag="QIE41_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDS3530208.1"
                     /translation="MKIILLFLAALASFTVHAQPPSHTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTMQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21853..22110
                     /gene="yqhH"
                     /locus_tag="QIE41_001300"
     CDS             21853..22110
                     /gene="yqhH"
                     /locus_tag="QIE41_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDS3530209.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22152..24371)
                     /locus_tag="QIE41_001301"
     CDS             complement(22152..24371)
                     /locus_tag="QIE41_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDS3530210.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMNTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGMKKRPKAAVGR"
     gene            complement(24482..25894)
                     /gene="ftsP"
                     /locus_tag="QIE41_001302"
     CDS             complement(24482..25894)
                     /gene="ftsP"
                     /locus_tag="QIE41_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDS3530211.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGNDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25969..26706)
                     /gene="plsC"
                     /locus_tag="QIE41_001303"
                     /gene_synonym="parF"
     CDS             complement(25969..26706)
                     /gene="plsC"
                     /locus_tag="QIE41_001303"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDS3530212.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIADLDKEVAEREAAGKV"
     gene            complement(26940..29198)
                     /gene="parC"
                     /locus_tag="QIE41_001304"
     CDS             complement(26940..29198)
                     /gene="parC"
                     /locus_tag="QIE41_001304"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDS3530213.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNATAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIEQPNTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMEQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLREILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29744..30226)
                     /locus_tag="QIE41_001305"
     CDS             complement(29744..30226)
                     /locus_tag="QIE41_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDS3530214.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(30279..30671)
                     /gene="ygiW"
                     /locus_tag="QIE41_001306"
     CDS             complement(30279..30671)
                     /gene="ygiW"
                     /locus_tag="QIE41_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDS3530215.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30823..31482
                     /gene="qseB"
                     /locus_tag="QIE41_001307"
                     /pseudo
     CDS             30823..31482
                     /gene="qseB"
                     /locus_tag="QIE41_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
     gene            31479..32828
                     /gene="qseC"
                     /locus_tag="QIE41_001308"
     CDS             31479..32828
                     /gene="qseC"
                     /locus_tag="QIE41_001308"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDS3530216.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDITLNADSFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNVEQGGFETKVSW"
     gene            32938..33519
                     /gene="mdaB"
                     /locus_tag="QIE41_001309"
     CDS             32938..33519
                     /gene="mdaB"
                     /locus_tag="QIE41_001309"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDS3530217.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33550..33864
                     /locus_tag="QIE41_001310"
     CDS             33550..33864
                     /locus_tag="QIE41_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDS3530218.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33909..34796)
                     /locus_tag="QIE41_001311"
     CDS             complement(33909..34796)
                     /locus_tag="QIE41_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDS3530219.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRTVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(34793..35740)
                     /locus_tag="QIE41_001312"
     CDS             complement(34793..35740)
                     /locus_tag="QIE41_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HDS3530220.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGAKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(35751..36788)
                     /locus_tag="QIE41_001313"
     CDS             complement(35751..36788)
                     /locus_tag="QIE41_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDS3530221.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNQVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEIGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(36797..37780)
                     /locus_tag="QIE41_001314"
     CDS             complement(36797..37780)
                     /locus_tag="QIE41_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDS3530222.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(37777..38586)
                     /locus_tag="QIE41_001315"
     CDS             complement(37777..38586)
                     /locus_tag="QIE41_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3530223.1"
                     /translation="MAHNAKGQGLILDNVSAGYHTKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         9637 a        10443 c         9715 g         8902 t
ORIGIN      
        1 caacatgtat tactctttca ccacaattaa cggttcaata tcactctctt ttttaatgac
       61 cagtgattca tcaccgcgca aacacgtccc accgtagttg ccgccaacgg tgaaggtaca
      121 tacctgaatg tatttaccgt ccactttcgg caaacaccac agttgctgat agatgttttt
      181 ctgctcagca aatttaccgc tggttttgtc cagcacctct tcatgatggc tgaccaggtc
      241 gatattgcta ccacagcgac cagcgatcgg tttcactgca taaccggttt tcaccagttc
      301 atcattcacg gagaaatcgg tatccagcag gtagcgatgg tgcgggaaca gcgaccagag
      361 gatcggcaga atcgctttgt tgccggggat caccgtccac agcggctcaa agaccagcac
      421 ttccgggcgc agcaatacgt cgataagacg cacttcattt tgcggatgac cggtacggat
      481 cggcaccgca gcaaactcac ggtcgctaac ttcacgaatc tgatcaaacg cggtttccca
      541 cgcccaggtt ttccacacgc agttaaccag tcgcccttcc ccatcaatca gttgcccggc
      601 agcatcccag cccagttcat ccagcccgcg caagatacgc gtttcaaagc ccgcctggtg
      661 cagcgcctgc tccataaact gcgcgtgata gttttcctcg atatctttgt cctgcatgat
      721 atggacaaac ggacgtgcac gactgtgttt ccaggcacca gccagttcgt taatcagccc
      781 ttccgccgga ttgaagccgt tgcctttata gccctgctcc gcccaacgtt cgaggatcaa
      841 gccagcttcg gtatgacagg aggcggagtc ggcgttgtat tcgtagacct tcaggccacg
      901 ctcatccatg cagaaatcca tacgaccggt gatcatatgg tgacggcgac gctgccagga
      961 gagacgcaaa cgtggccaga ggattttcgg aatgtcgaac agcgccagca ggttgtcatc
     1021 tttcagcacc ttgtcggttg cgtgaagata catcaggtgc agctcgttgg tggctttaat
     1081 cagctcctgc tcggcgctct cggtaatggt gtagtaatga tacggatcct gattgatcac
     1141 ctgaccgttg gcctgcacat aggcgttttg cagcggatct ttttcatcca gccatttacc
     1201 gtcaaactgg cctttgtttt ccagacgcgc tccgctgatt ttcagcagct cgcccgcaat
     1261 ttccggctgc ggtaagctgt attcggtatc ttccgtctgg atcatccagc ccagaatggt
     1321 ggtgtcatca aacgtgtctt taagggtata gcagccgttt tcgaccacca tctccagctc
     1381 gcgcgtccac tgttgtcctt gcggcaacgg ggaatgaatc acgttctgtt ccgcaatacg
     1441 gactttattg ccatgcaatt gggtaatgat ggcgacatgg ccggtgtctt taaattcacc
     1501 gcctttatcc cagataagaa gcgcgcccgc gaccggtgca cgcggcgagc cattaggaaa
     1561 tgcctgcaat ggcaggatgt tgtcattaac cacttcacgc aggaagcgca gcgagaaaat
     1621 ctcccacgcc atacccacgt cagtgaagac cacaccgtaa ttcagaaaga gaaaacggcg
     1681 agcaaattca acgcattgcc atttgtggcc catatattcg tcgtcgatat agctacggaa
     1741 tacggcgtca tcttcgtatt cctgcggatc gagagaactg taatctgaag agtagattgc
     1801 tacaccacct ggggcgtagc ccaataatgt cccgaacggg gcatcctggc tggtcgttcc
     1861 tttgctcatc actttacctt taacaataca acctaagcag ttggcgtaat tgttgtgctc
     1921 tgattacctg ctcagcgaca ttaaccggac agaggtcaga ctaatcatac acctcatcgc
     1981 agcggctggc gcaaagggtt aaaaaattca cattctgtac agcaagtgac ctgctacact
     2041 gcttcgacac taccactcag aaggcaactc actatgacag acaatactta tcagcccgcg
     2101 aaagtctgga cgtgggacaa atccgccggc ggcgcgttcg ccaatatcaa tcgcccggtt
     2161 tctggtccga cgcatgaaaa aacgctgccc gttggcaaac accccttgca actctattcg
     2221 ctgggaacgc cgaacggtca gaaagtaacg attatgcttg aggagctgct ggagctgggc
     2281 gttactggtg cagagtacga tgcctggctg atacgcatag gcgatggcga tcaattctcc
     2341 agcggctttg tcgaagtgaa cccgaattcg aagatcccgg cgctgcgcga tcatacgcat
     2401 aatccgccga tccgcgtgtt tgagtctgga tcgattctgc tttatctggc ggagaaattt
     2461 ggctacttcc tgccgcagga tctggcaaag cgtactgaag caatgaactg gttgttctgg
     2521 ttacagggtg ctgcgccgtt tctcggcggt ggttttggtc acttttacca ttacgcgccg
     2581 gtgaaaattg aatacgccat caaccgcttt accatggaag ccaagcgcct gctcgacgtg
     2641 ctggataagc aactggcgca gcataagttt gttgcaggcg atgagtacac cattgcggat
     2701 atggcgattt ggccgtggtt tggcaacgtg gtgttaggcg gcgtgtatga tgccgcagag
     2761 tttctggatg cgggaagtta taaacatgtg caacgctggg cgaaagaagt gggcgaacgt
     2821 ccggcagtga agcgtgggcg tattgttaac cgcaccaacg ggccgctgaa cgagcagttg
     2881 catgagcgcc atgacgccag tgattttgag acgaatacgg aagataagcg tcaggggtaa
     2941 gggttggtgt tcgtcgcagc aagccatcca ggccgaggta ggccggaaaa ggcgttcacg
     3001 ccgcatccgg caatcgtgca taatgcctga tgcgacgctg ccgcgtctta tcaggcctac
     3061 aatcggcacc cgaacggtag gccggataag gcgttcacgc cgcatccggc aatcgtgcac
     3121 aatgcctgat gcgacgctgt cgcgtcttat caggcctacg aaagctaatc atcacgcgct
     3181 ggtaatgtcg taatccattt ggcgcagcgc gtctaatgtg gctttggctt catcttcgtc
     3241 gatgatgctc atggcaaatc cgacgtgtac cagcacccac tggcccagca gatcggcagg
     3301 gttaccttca caaatcaggg cgatattcac gtcgcgcttg ataccacata cttcaacctg
     3361 cgcaagctgg tgaatatcct caccgacagc cagcacctgg cctggaacgc caatacacat
     3421 tgttaactcc ggttattcaa cttcaatact tttgacgatc agcgaatcgc cggtatcgac
     3481 acgcaaccgc tcgccatgac agagcggaca ctgcgcatcg tgctgatgaa tctccaccac
     3541 ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta aatcgcaccc
     3601 ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc tctcctcaac
     3661 gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat cgtactgctc
     3721 cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat gcattttcgc
     3781 cactcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc ggattcgtga
     3841 ctggcagcga aaggatcatt cgtgcgcagt catccgtcag gcgttggccc tcttcaatcg
     3901 acatgctgtg cgaaagcggc gacatcagcg agcaggagag gtattgcgaa acaccgtcca
     3961 gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt ttttcactca
     4021 ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc agcatccacg
     4081 gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaagtcagaa acatacaccg
     4141 gcattgaagg atgcagaaaa gagagatcat gcatcgaacg ccgggcaatt tcttcaaaag
     4201 ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca gtcatgagtc
     4261 gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac ctgctcaagc
     4321 gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc cagcgattcc
     4381 gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg cagggccgac
     4441 aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa cgccggaact
     4501 tcttcatccc gcaggatcat catcgttccc ggcgcgctct tcttcgacac aatggcatcc
     4561 gcaatgatca gatgatcgcg atttgccatg tcgccaagca gctccattcc cgccgtgccg
     4621 ccatcgagga tctcaacata atccggcaga atatatcgtt gctctaacgc ttcgacaatc
     4681 cgcacaccga tggcttcatc ggtcagcaaa atattgccaa cccctaagac taaaatacgc
     4741 attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg tgtaccgcac
     4801 aggccatgca cggatcgaag gagtgaatgg tacgcaccac ttccagcggt ttattcggat
     4861 cagcaaccgg tgtacccacc aacgactgct cgtaaggacc gacgtcatca ttgaagttac
     4921 gcgggccaga gttccaggtt gatggaacaa ctgcctggta gttgctgatg ataccgtctt
     4981 tgatcaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca acacctttga
     5041 actcacccgt tgccggaatg ttcggtttca cgaaggtggt gtgatcgcct ttgccgatat
     5101 tggtgatcag tgcactgtat tgattttgca ggatatcctg caattcgcag cagtgaacgg
     5161 tacgaccaat aatacggccc agcgtggagt gcagctgcgc cacttccagc gtgttgccag
     5221 tcagtttctg ataaatcgca acgatttcat tcagtttatt ctgggtagat tcgcgacctg
     5281 ccgccagttt caccagcatg ttagccagcg gccccacttc taccgttttg ccgtagaaag
     5341 tcggtgattt cacccaggag tatttaccgt cgtcagacca accatcataa gccggaatgg
     5401 tggtaccttc ccacggtgcc tgcggcgctt cgtctttata ccaggagtgc ttcgcgcttt
     5461 cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga tacgaggaca
     5521 gatccgcatt ctcaatgtag ccgcccggga acaggaagct gccgttttta ctgtcggtcg
     5581 ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc cattccgggt
     5641 agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag tcgctcagct
     5701 tgtcgatgaa ggacttgatg tacatcagac gctcaagatt cagcacgccc agaccgtcga
     5761 ggttgatcgg gttagcgacc ccacctactg ccaggttctg aatgtgcggc gttttaccgc
     5821 ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg
     5881 ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc cagtagccat
     5941 tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc tgaactttag
     6001 tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt tcggaggctt
     6061 tggttgggtc agcttgcagt gcagaagtga tatccaccca gtccagcgcc gaaagttgat
     6121 agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt
     6181 gcgcgttaac cggaacgtcg atattcagcg cactctctgc cgcacgaacg gaagacagcg
     6241 cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca tcgcgcggat
     6301 cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc catgctttcg
     6361 aaacgacacc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg
     6421 gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat gatcgttttg
     6481 ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc aaatctcaat
     6541 agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg cgtaaccgcc
     6601 gcccgggttg aatgccacca gcgaataggt cagacgccag gttgcacaac ctaacagtgc
     6661 gctcagtgct gacaggaaca gcatgcggga atcattacgc agcttcgcca cacgcagaac
     6721 gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt agaagtcacc
     6781 ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca gcacgatgaa
     6841 aatcgccagc aacacactga tggtgttggt cagcttaacg aacagactct tttcatccgg
     6901 accgttgcca cgcagacccg cctgcaccag cgaaccttca aagatgacaa tcgagaagcc
     6961 catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct gccacagcgg
     7021 atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt gcatagttgg
     7081 cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgct gtagcgacac
     7141 cttccagccc aatcgttcaa acaatgccgg agcaaactcc agtgccatca cgccgatgta
     7201 gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt gacccggaat
     7261 gtagaagtac ggcaggttcc agtagcgacc aacgtcgatg gtaatcgaca agccacccag
     7321 tgagtaaccg aacagactcg ccaacagcgc cggacgcacc agcggatggt attgcccacg
     7381 gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc cggtgccgat
     7441 caacaggtca aacgcgatcc acacacccca cgggaagccg ccgttcaggt cagagactga
     7501 gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga ttaacggtcc
     7561 aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat catgactcat
     7621 gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc acggttaagc
     7681 ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttgg acatgttcgg
     7741 aacgcgcacc agtagaaaga tcgtccagtt tcggcaggtc gagattttca taaggcacac
     7801 cggtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga tgcggataat
     7861 atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc ggatagtggt
     7921 attcgctgcc tggcttcagc gccagacgtt ttttcgcctc agccatcagc tcttcacgcg
     7981 taccaaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga ccgcctttat
     8041 caagacgttc cacacctttc tggttgcaca gttcacactt atgcagcgca ccaaatgggt
     8101 tgttgtagtc gtacttcggc acgttgtatg ggcaggcgac catgcagtaa cggcagccag
     8161 tgcacacatc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc agcgcagaga
     8221 ccggacacac ggagacacag ttcggatcga cgcagtgcat acactgtttc ttaatgtacg
     8281 cgtagccgtt ctcttcctgg tctttgttga cccctgtgcc gctggtccac acctgaatga
     8341 tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt
     8401 tacgttcagg gaaattgata tcctgacact tggtgacgca agcctggcag ccaacgcaca
     8461 aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt tcagcagccg
     8521 catgactgac agacggcaac gcgcccgtca gcaatgcccc gcaggaggct gctttgataa
     8581 aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct tttgctgacg
     8641 acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca caccgccgag
     8701 caaaccaata gcgcctgcag agacgttgcc gccttcttta gcgttaacgt ccggtttctg
     8761 tgaacgcgga gtctggtttt cgacgttggc aagctgatgg atgcctttat ggaagccgat
     8821 accttcttcg ttacagccat agcacgggtg accaatcgcc accggccaca cgccgccaac
     8881 gtcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac agccgaggtg
     8941 gtacaggcac cagccttcac ggtggccttc gtcgccgaac tctttcgcaa agcgaccagc
     9001 atcgaagtgc gggcgacgtt cacagtgttc gtgaatcaga cgaccgtagg cgaaagtcgg
     9061 acggttttta tcatccagct tcggtggttt gccgtaagtg atgatgtgcg caacggtcgc
     9121 gaggaagttg tgcgggttcg gcgggcagcc aggaatgttg attacagttt tgcctggcag
     9181 aacttcttgc agactgaccg cgccagttgg gttaacccca gccgcggcaa caccgcccca
     9241 cgcagagcag gaaccgatag cgataatggc tgctgcgcct tccgccgctt tgcggatgtg
     9301 atccacaatc ggctcaccgg caaccatgca ataaatacca ttatctttta atgggatgga
     9361 accatccacc actaacacat actgcccttt gtacttctcg agagcgttat gtttgttctc
     9421 ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag agatagtctc
     9481 cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg tacaaccggt
     9541 gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa ccgattcggc
     9601 catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg cacaaagctt
     9661 catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc cagtcattta
     9721 tagttattcc gttgcgaaga cctggcatat attttgcctc aatcgcaaaa tcaataatgc
     9781 gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat actgcggcgg
     9841 ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat aatacgtatg
     9901 tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga acaggaatta
     9961 ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg cgtcagaata
    10021 cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatccg gtgtgagtag
    10081 cctaatacaa aaccgcgttt ataatcggca cagtattttg tcacattaac cgcactgtcg
    10141 gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt catcgttcac
    10201 ctcattcgct atatattgcc atatatagcg atattttagg caaagaacga cttttgcgca
    10261 cgctattttt tcatgcttgt ttaagacgta atcccgtaag agataaacct atcaggagag
    10321 atatttatcg aaccatttca gcgtcctttg ccaggcaaga tcggcggcag atttgtcata
    10381 acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat acgcctcata
    10441 gaccttatta ttggctttca acgccgcctc gtaagcaggc cagccctcgt tgattcgggt
    10501 gtccagttcc gcgtagtgga gtagtaaagg cgcttcaatc ttcgccacat cggcggtggg
    10561 tgcctgtcga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga cagccgccgc
    10621 gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttgcctg tcgcttgcgg
    10681 atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac
    10741 ctgctgttgc agctcacgac ctttatcatc attacccgga taacctccaa cggaacttaa
    10801 gccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca catcttcgat
    10861 atacggattc agtccacgat tctcatgcac caccactacg gctggcgttt tgccgctcat
    10921 ctttgcgggc ttcaccagat aaccccgcac ctcgccgtga ccatttggtg aaggatacgt
    10981 gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca gcgcataatt
    11041 tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cctaccgcat acttcgccgc
    11101 aaggttgagg aactcacgtt tcgagatttt cccgtgagcg taatagtcat agtaatccag
    11161 caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcactcc tcagttggtg
    11221 tttttttaag caaagcataa gcgcgtgttt ttgcccaatt tttcgtcact ctgtgagcca
    11281 gactacggga tacgcgctga tgaatcgcta aactagaaac attgtttcga aattgaacgg
    11341 tggaaaggag aggtcatggt ctggttagcg aatcccgagc gttacaggca gatgcagtac
    11401 cgctattgcg gaaaaagcgg tttacgcctg cccgcgttat cgctcggttt atggcacaat
    11461 ttcggtcacg ttaacgcgct ggaatcacag cgtgcgctcc tgcgtaaagc gtttgattta
    11521 ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag cgcagaagag
    11581 aactttggtc gcctgctgcg ggaggatttt gccgcttatc gcgatgaact gattatctct
    11641 accaaggctg gctacgatat gtggcccggc ccttacggct ctggcggttc acgtaaatac
    11701 ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt cgatatcttt
    11761 tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc gctggctcat
    11821 gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc agagcggacg
    11881 caaaaaatgg tcgagttact gcgcgagtgg aaaattccgc tgttaattca tcaaccttcg
    11941 tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct gcaaaataac
    12001 ggcgtgggct gcattgcctt tactcctctg gctcagggat tgctgaccgg aaaatatctc
    12061 aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg tggtctgacg
    12121 ccgaaaatgc tcaccgaagc caacctcaac agcctgcggt tattgaatga aatggcacag
    12181 cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg
    12241 acgtcggtat tgattggtgc cagccgcgcg gagcaacttg aggagaacgt gcaggcgctg
    12301 aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat cgccgatggc
    12361 gagctgaatc tgtggcaggc gtcttccgat aaatgatctg ttaataacgg gcgcgagaac
    12421 cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc ccatcacaaa
    12481 tgcagagagc acaaacgtca gatggataat gacgtaccac atcagtttgt tatcagggac
    12541 atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc
    12601 tgctactttg tttttcagcg acgttgcgtc cattttcccc agccagttca gcttctcttt
    12661 gttctcggag atatccagct gcgagacgaa attctcataa ccggaaaaca tcaccatcac
    12721 cagcaaaccg ccaaccagcg tcatatccac cagtgacagc aacacgagga tcaaatctga
    12781 ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga acttcagcgc
    12841 cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc
    12901 atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta aaacgggcaa
    12961 cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatcc gttaaacctt
    13021 tgtcgcataa atccgaggtt tagggatctt cctggttccc ttatcaacgc atcgagagcg
    13081 tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt taaaagaccc
    13141 gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc caggcatcca
    13201 ggcgaagatg acaccggtac cggattgcgg cgagaaaacc tatgttggta gcggtcgcct
    13261 gaaagatcgt aaagcactgg tgacaggggg cgattccgga atcggtcgcg ctgccgccat
    13321 cgcttacgcg cgtgaagggg ccgacgtggc gatcagttat cttcccgtgg aagaagaaga
    13381 cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgtcc tgctgccagg
    13441 cgatttaagc gatgagaaat ttgcccgttc gctggttcac gaagcgcata aggccttagg
    13501 cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattcctg atattgcaga
    13561 cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcac tgttctggct
    13621 aacccaggaa gcgatacccc tgctaccgaa aggtgcaagt atcatcacca cttcgtcaat
    13681 ccaggcatac cagccaagcc cgcatttact ggactatgcg gctacgaagg cggcgattct
    13741 gaactacagc cgaggcctgg caaaacaggt cgcggagaaa ggtattcgtg tgaatattgt
    13801 tgcacctggc ccgatctgga cagcactgca aatttccggc ggacagacgc aggataagat
    13861 cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg aactggcccc
    13921 tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag tgcatggcgt
    13981 gtgcggcggc gagcatttag gctaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca
    14041 tttttatttc gctttggcag tttcttcgcc gaccagacct atcttcagat aacccgcctg
    14101 atgcagcgta tccattacct tcatcaacgt ctcgtaatcg acggttttgt ccgctcggaa
    14161 gaagatggtg gtgtctttct tgccttcggt taacgcattc aacgccgtaa tcattgtttc
    14221 atcggtgacc ggatcgttac cgataaacat cgagttgtct gccttcaccg acagataaac
    14281 cggtttttcc gggcgcggct gcggcgtgct ggtagaagca ggcaagttca ccttcacatc
    14341 taccgtcgct aacggtgccg ccaccataaa gataatcagc agaaccaaca tcacgtcgat
    14401 aaacggcgtc acgttgatat catgcatttc gccgttatcg tcgaggtttt cgttaagatg
    14461 cattgccatc ggacattatc ctgcgcgtaa tttttgtgcg acacgaaccg gatgcgcagc
    14521 ggcgctggct tccagatcca ggtcacggct ttgcagcaac aatacctgcg ctgcgacatc
    14581 acccagcatc gctttaaagc cgccaatctg acgtgcaaat acgttataga taacgaccgc
    14641 cggaatcgct gccacgaggc cgattgccgt tgctaacaga gcttctgcaa tacccggcgc
    14701 aacgactgcc aggttagtgg tctgcgtttg cgcgatacca ataaagctgt tcatgatgcc
    14761 ccagaccgta ccaaacagac caacgaacgg cgaaatcgcg ccgatggttg ccagatagcc
    14821 gttaccgcgg cccatttgac gacctactgc ggcgacccga cgctccagac ggaagctggt
    14881 acgttcttta ataccttcgt tatcgtcgct gccttctgac agttccagct cattctgcgc
    14941 ttcattgagc aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga tatcgttggc
    15001 ctggtttaag gaacgcgctt cagccagcag ttgctgctcg cgcttaaggc gacgcttctg
    15061 attgaagaac tctacgctct tactgaagaa gattgcccag gtgactacgg aggccaaaat
    15121 aagcccaatc atcacgcact taacgacaat atcggcgtgc tgatacatac cccagacgga
    15181 aaggtccgtc tgcattaaat tattacccac tgtgtatctc caggacgcaa gtcacaaaat
    15241 ctgcgcataa taatatcaaa acgacgtcga attgatagtc gttctcatta ctatttgcat
    15301 actgccgtac ctttgctttc ttttccttgc gtttacgcag taaaaaagtc accagcacgc
    15361 catttgcgaa aattttctgc tttatgccaa ttcttcagga tgcgcccgcg aatattcatg
    15421 ctagtttaga catccagacg tataaaaaca ggaatcccga gatggcggac aaaaagcttg
    15481 atactcaact ggtgaatgca ggacgcagca aaaaatacac tctcggcgcg gtaaatagcg
    15541 tgattcagcg cgcttcttcg ctggtctttg acagtgtgga agccaaaaaa cacgcaacgc
    15601 gcaatcgcgc caatggcgag ttgttctatg gacggcgcgg aacgttaacc catttctcct
    15661 tacaacaagc gatgtgcgaa ctggaaggtg gcgcaggctg cgtgctgttc ccctgcgggg
    15721 cggcggcggt tgctaattct attcttgctt ttgtcgaaca gggcgatcat gtgttgatga
    15781 ccaacaccgc ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc aaactgggcg
    15841 taacgacatc atggtttgat ccgctgattg gcgcagatat cgttaaacat gtgcagccaa
    15901 acactaaaat cgtgtttctg gaatccccag gctccatcac catggaaatc cacgacgttc
    15961 cggcgattgt tgccgccgtg cgcaatgtgg tgccagatgc catcattatg atcgataaca
    16021 cctgggcggc cggtgtgctg tttaaggcgc tggattttgg catcgatgtt tccattcagg
    16081 ccgctaccaa atatctggtg gggcattcag atgcgatgat tggcactgcc gtgtgcaatg
    16141 cccgttgctg ggagcagctg cgggagaatg cctatctgat gggccagatg gtcgatgccg
    16201 ataccgccta tataaccagc cgtggcctgc gcactttggg tgttcgcctg cgtcaacatc
    16261 atgaaagcag tctgaaagtg gctgaatggc tggcagaaca tccgcaagta gcgcgagtta
    16321 accaccctgc tctgcctggc agtaaaggcc acgaattctg gaaacgagac tttacaggta
    16381 gcagcgggct attttccttt gtgcttaaga aaaaactcaa tgatgaagag ctggcgaact
    16441 atctggataa cttcagttta ttcagcatgg cctactcgtg gggcggttat gaatcgttga
    16501 tcctggcaaa tcaaccagaa catatcgccg ccattcgccc acaaggcgag atcgatttta
    16561 gcgggacctt gattcgcctg catattggtc tggaagatgt cgacgatctg attgccgatc
    16621 tggacgccgg ttttgcgcga attgtataac attgccactt ttggacaatt ttgcagacat
    16681 tttattgtga aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg acgattcagg
    16741 agtacaatag gcagataaag gcttaaacgc tgttccacag gaaagtccat ggctgttatt
    16801 caagatatca tcgctgcgct ctggcaacac gactttgccg cgctggcgga tcctcatatt
    16861 gttagcgttg tttactttgt catgtttgcc acgctgtttt tagaaaacgg cctgctgccc
    16921 gcctcatttt tgccaggcga cagcttgttg atattggctg gcgcattgat tgcccagggg
    16981 gttatggatt ttctgcctac gattgcgatt ctgaccgccg cagcaagtct gggctgctgg
    17041 ttaagttata ttcaggggcg ctggttaggg aataccaaaa cggtgaaagg ctggctggca
    17101 cagcttcccg ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca cggtctgctg
    17161 gcgctgctgg ctggacgttt tcttgcattt gtccgtacgt tgctgccaac catggcggga
    17221 atttccggtc tgccaaaccg ccgcttccag tttttcaact ggttaagtgg attgctgtgg
    17281 gtcagcgtgg taaccagttt cggctatgcc ttaagtatga ttccgttcgt taaacgccat
    17341 gaagatcagg taatgacgat cctgatgatc ctgccaattg ccttgttaac cgctggcttg
    17401 ttaggcacgc tgtttgtggt gattaaaaaa aaatactgta acgcctgacg attttccccg
    17461 ttcccggttg ctgtaccggg aacgtattta attcccctgc atcgcccgca ttcttgccgc
    17521 atcttccccc ggcgtcacac caaagtaacg tttaaactca cggctaaatt gcgatgcgct
    17581 ttcgtaaccg acgcgcatcg ctgccgcact ggctttcatg ccgtcgtgga taatcatcat
    17641 ccgcgcctta tgcagacggt aattcttcaa atactgcaac ggcgaggtgc tggtgacaga
    17701 cttaaaatta tggtggaacg ccgatacgct catgttggct tctgccgcca gttgctcgac
    17761 gctcaggttt tcggtgtatt tattctcaat ccgtttcagc acgcggctaa tcaggctgaa
    17821 gtgagtctgg cgactgacca gcgccagtaa cgcgccgccg caaggtccgg tcagcacgta
    17881 gtacagaatt tcgcggatga tctgtttgcc gagaatacgc gcatccagtg gtcgctccat
    17941 cacgtcgagt aaccgctccg ccgcgcataa aatctcttct gataacgtgg cggagttaat
    18001 cccgctggct gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca tcaacagttc
    18061 ctgtaactgc aaaatatcga cattgagacg caaccctgcc agcggcacct ctgacgtggc
    18121 ataggtttcg cactcaaacg gcaacggcac cgtcagcagc aggtattcat tggcatcata
    18181 acgaaacacg cgttcattga tataaccgat tttatgtccg gaaaagagaa ttatgatgcc
    18241 aggctcgtac atcaccggtg tacgtgcgaa aggcgtctcg ccatacaaca aacgcacatc
    18301 gggcaacagt tctgacaaac tattttcttt atttttcagt ttattaactt tatccgccag
    18361 caagcggcaa atctcttcac gtttcatatc gcgtaatttc ttaggaataa tgcggcaatt
    18421 tgattgtgcg caattttgta gcatttctcc agcactctgg agaaataggc aagacattgg
    18481 cagaaatgag cattgagagc cagggcgctg gcgatcacaa tgaaaaacat caggcagatc
    18541 gttctctgcc ctcatattgg cccagcaaag ggagcaagta atgaacaact ttaatctgca
    18601 caccccaacc cgcattctgt ttggtaaagg cgcaatcgct ggtttacgcg aacaaattcc
    18661 tcacgatgct cgcgtattga ttacctacgg tggcggcagc gtgaaaaaaa ccggcgttct
    18721 cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg gaatttggcg gtattgagcc
    18781 aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg gttcgcgaac aaaaagtgac
    18841 tttcctgctg gcagttggcg gcggttctgt actggacggc accaaattta tcgccgcagc
    18901 ggctaattat ccggaaaata tcgatccgtg gcacattctg caaacgggcg gtaaagaaat
    18961 taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca gcaaccggtt cagaatccaa
    19021 cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag caggcattcc attctgccca
    19081 tgttcaaccc gtatttgccg tgctcgatcc ggtttatacc tacactctgc cgccgcgtca
    19141 ggtggctaac ggtgtagtgg atgcctttgt gcacaccgtg gaacagtatg ttaccaaacc
    19201 ggttgatgcc aaaattcagg accgtttcgc agaaggcatt ttgttgacgc tgatcgaaga
    19261 tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg cgcgccaacg ttatgtgggc
    19321 ggcgactcag gcgctgaacg gtttgatcgg cgctggcgta ccgcaggact gggcaacgca
    19381 tatgctaggc cacgaactga ctgcgatgca cggtctggat cacgcacaaa cactggctat
    19441 cgtcctgcct gcactgtgga atgaaaaacg cgataccaag cgcgctaagc tgctgcaata
    19501 tgctgaacgc gtctggaaca tcacagaagg ttccgacgat gagcgtattg acgccgcgat
    19561 tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg acccacctct ccgactacgg
    19621 tctggacggc agctccatcc cggctttgct gaaaaaactg gaagagcacg gcatgaccca
    19681 actgggcgaa aatcatgaca ttacgctgga tgtcagccgc cgtatatacg aagccgcccg
    19741 ctaagctttt tacgcctcaa actttcgttt tcgggcattt cgtccagact taagttcaca
    19801 acacctcacc ggagcctgct ccggtgagtt catataaagg aggaacgtat ggctaatcca
    19861 accgttatta agctacagga tggcaatgtc atgccccagc tgggactggg cgtctggcaa
    19921 gcaagtaatg aggaagtaat caccgccatt caaaaagcgt tagaagtggg ttatcgctcg
    19981 attgataccg ccgcggccta taagaacgaa gaaggtgtcg gcaaagccct gaaaaatgcc
    20041 tcagtcaaca gagaagaact gttcatcacc actaagctgt ggaacgacga ccacaagcgc
    20101 ccccgcgaag ccctgctcga cagcctgaaa aaactccagc ttgattatat cgacctctac
    20161 ttaatgcact ggcccgttcc cgctatcgac cattatgtcg aagcatggaa aggcatgatc
    20221 gaattgcaaa aagagggatt aatcaaaagc atcggcgtgt gcaacttcca gatccatcac
    20281 ctgcaacgcc tgattgatga aactggcgtg acgcctgtta ttaatcagat cgaacttcac
    20341 ccgctgatgc aacaacgcca gctacatgcc tggaacgcga cacacaaaat ccagaccgaa
    20401 tcctggagcc cattagcgca aggagggaaa ggcgttttcg atcagaaagt cattcgcgat
    20461 ctggcagata aatacggcaa aaccccggcg cagattgtta tccgctggca tctggatagc
    20521 ggcctggtgg tgatcccgaa atcggtcaca ccttcacgca ttgtcgaaaa ctttgatgtc
    20581 tgggatttcc gtctcgacaa agacgaactc ggcgaaattg caaaactcga tcagggcaag
    20641 cgtctcggtc ccgatcctga ccagtttggc ggctaacgtg caaattctcc cggtggcggt
    20701 aatgttccgc taccggactt ttcagaaatc atttattccc ctcgcgtcct gcccattgtt
    20761 actcttcctt gttcaggaat gccaaatata aggacatcat catgcagagc cggaagctct
    20821 taaaggaaca actcatctac acccgggata aacgcaacgg agaggtgaaa aacagatgaa
    20881 aataatactt ctgtttttag cagccctggc aagttttacc gtacacgcgc agcctccttc
    20941 acacaccgta gaacaaacag tccggcagat ttatcagaac tataaatcag atgccagtac
    21001 gccttatttc ggtgaaaccg gagagcgggc gataacttca gcgcgtattc aacaggcgct
    21061 taccctgaac gacaatctta cactaccggg caatattggc tggctggatt atgatccggt
    21121 ttgtgattgt caggattttg gcgatctggt gttagaaagc gtagcgataa cccaacctga
    21181 cgccgatcat gccgatgccg ttgtgcgctt tcgtatcttt aaagacgata aagaaaagac
    21241 catgcagaca ctgaaaatgg tggcggaaaa tggtcgttgg gtcattgacg atattgtcag
    21301 caatcatggc agcgtcttac aagcagttaa tagcgagaat gaaaaaacgc tggccgctat
    21361 agcttcgttg caaaaagaac agccggaagc ctttgttgcc gaactctttg aacatattgc
    21421 tgattatagc tggccgtgga cgtgggtggt ttccgactct taccgccagg cggtcaatgc
    21481 cttctataaa accaccttca agactgccaa caatcccgat gaagatatgc aaatagaacg
    21541 gcaatttatt tacgacaatc cgatctgttt tggcgaagag tcgctatttt cacgtgttga
    21601 tgaaattcga gtcctggaga aaaccgccga ttccgcccgc attcatgttc gttttacgct
    21661 gaccaatggc aacaacgaag agcaagaact ggttttacag cggcgcgaag gtaagtggga
    21721 aatcgctgat tttatccgcc cgaacagcgg cagcctactt aagcaaattg aggcaaaaac
    21781 cgccgccaga ttaaagcaat gagctgaatt aaataacaat tagccggaac aataaataaa
    21841 agggaacact atatgaaaac gattttcacc gtgggagctg ttgttctggc aacctgcttg
    21901 ctcagtggct gcgtcaatga gcaaaaggtc aatcagctgg cgagcaatgt gcaaacatta
    21961 aatgccaaaa tcgcccggct tgagcaggat atgaaagcac tacgcccaca aatctatgct
    22021 gccaaatcgg aagctaacag agccaatacg cgtcttgatg ctcaggacta ttttgattgc
    22081 ctgcgctgct tgcgtatgta cgcagaatga taaaaatatc cccggtagca ttctgttacc
    22141 ggggatattt tttaacgtcc aaccgccgct ttagggcgtt tcttcatacc agcattcacc
    22201 ggacgagatt gcgtagacga cgcttttttt gccgtagcag gcgtctgacg ctgggtcgcc
    22261 atcggcgtat gtttagtcaa cgccggacgg gtattgcggt tctggcgacg agcctcacgc
    22321 atctcttcaa tggttggcgc aggcactaag caatcgcgac ggctgccaat cagatgcttt
    22381 ttgcccatcg cttccagcgc ctgacggatt aacggccagt ttgccggatc gtggtaacgc
    22441 aacaacgctt tatgcaaacg acgctgcttg tcgcccttcg gtacgaagac gtcttcgctc
    22501 ttataaccaa tcttcgccag cgggtttttc ccggtgtaat acatggtggt tgagttcgcc
    22561 agcggcgacg gatagaagtt ctgcacctgg tcgaggcgga agcggtgctt tttcagccac
    22621 agcgccagat tcaccatatc ttcatcgcgc gtaccggggt gtgccgagat gaaatagggg
    22681 atcaaatact gctctttacc tgcctgtttc gagtaggtat cgaacaactc tttaaagcgg
    22741 tcatagctgc ccatgcccgg cttcatcatc ttcgacaacg gcccttcttc ggtatgttcc
    22801 ggggcaatct tcagataacc gccgacgtga tgggtcgcca gctctttgat atagcgcgga
    22861 tcttccacgg cgatgtcata acgtacgccg gaagcgatca agatcttttt aatgcctttc
    22921 agatcgcgcg cacggcgata gaggttgatc gtcggttcgt ggttagtgtt catatgcgga
    22981 caaatatccg gataaacgca cgacaaacga cggcaggttt gctcagcgcg tggcgatttg
    23041 cagcgcaaca tatacatgtt ggcagttggc ccaccgagat cggaaattac gcccgtaaaa
    23101 cctggaacgg tgtcgcggat cgcttcgatc tcattgatga tcgaatcttc cgaacggctc
    23161 tgaataatgc gcccttcgtg ctcggtgata gaacagaaag agcagccgcc aaagcagccg
    23221 cgcataatgt tgaccgagaa acggatcatt tcgtaagccg gaatacgggc attgccatag
    23281 gccggatgcg gcacgcgctt gtacggcagc gcaaaaacgc tgtccatctc ttcggtagag
    23341 agcggaatag caggcgggtt gatccacaca tagcggtcgc catgtttttg catcaatgcg
    23401 cgggcgcagc ctgggttagt ttcgtggtgc agaatacgcg aagcatgggc gtacagcact
    23461 ttatcgccct tcactttctc gaaagaaggc agcaatacgt aggttttttc ccacggtttc
    23521 gggcgcggcg gctgcacggt tacggctttc gcttcctgct ttttcggcgc aacaggtttg
    23581 ttatccgcgc acggcagatc ttcgccatac ggatgcggga tcgggtcgat tttccctggg
    23641 gtatcgagac gggtggaatc cacgccgctc cagcctggca acgcctcttt tacgataatc
    23701 gcggtattac gcacatcgcg gatttcacta atcggctcgc ccatcgccag acgatgcgcc
    23761 acttccacca gcggacgctc accgttacca aacatcagca tgtcggcttt ggaatccacc
    23821 agcacagaac ggcgcacggt atcggaccag taatcataat gcgcggtacg gcgcaggctg
    23881 gcctcaatac cgccgaggat caccggcaca tctttccacg cctctttaca acgctgggta
    23941 taaaccagtg tggcgcgatc cggtcgctta cccgcgacgt tatccggcgt gtaggcatcg
    24001 tcatgacgta aacggcgatc ggcagtatag cggttgatca tcgagtccat gttgccagca
    24061 gtaacaccga aaaacagatt cggtttaccc agacgcataa agtcgtcttt gctgctccag
    24121 tccggttggg caatgatccc gacacgaaag ccctgcgctt ccagcatacg accgcaaatc
    24181 gccatcccga agcttgggtg atcgacatac gcgtcgccag taaccaaaat gatgtcgcag
    24241 ctatcccagc caagttgatc catctcttca cgagacatcg gcaaaaacgg tgccggtcca
    24301 aaacaggcgg cccagtactg cggccaggag aacaggtcgc gatccggttg gatcagggag
    24361 atagagctca ttttgcttcc agaaatgata aaaaaataat caaaggccgg ggattataag
    24421 ccggaacgaa agagaaatcg aaaggtattc catactcgcc ctcctcgggc gagtatgaag
    24481 attacggtac cggattgacc aacagttgcc ctatcgagcc acggtccgcc atttccagcg
    24541 tctgactgtt gaagtagaac gggaagtgcg cccaggaagg ctgaccgaaa taaacaagca
    24601 gctccacttg tccgtcaacc caaacggtat ctttccagcc tctgtcttcc gggaacggca
    24661 tcgcgccatt cacgttacgg atctggaaca tcacgccttc aatatggaac gcttgcggct
    24721 cgtccgcgcg taccgtccag cgttcccatg ttccttgctg cgcggtgaca tcaatacggt
    24781 taacgtccca cagttgtcca ttaatacccg ggtcatcacc cagactgata tcacgactgc
    24841 gaatcggcga accagccatg atttcagttg gcagcaagcg cataggaaga ctgtctgtga
    24901 ccagcggcag aaggccggtt gggcgcagcg ttagcaccag cgtagaaacc agaatgctgg
    24961 atggctcaaa gaagccacga atacgatcaa caatgctcgc cgcttcgcca caggtgatcg
    25021 acacttcatc accgttgctc atatccacca gaatctcacg acgctcgccc ggtgccagcg
    25081 aaagttgctt caccgataca ggagcgggca ggaatccctg atcgccagaa atcacatgta
    25141 acgggcgacc atcgctcatc tgtagttgat agcgacgaga gttcgacgcg ttcaacagac
    25201 gcaagcgcac ccagccacgc gagacttcaa cgtacgggct ttgtacaccg ttaaccagca
    25261 gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta ttctggcgta ccaaagttat
    25321 ccagccgttt atcctgaata atgaccggaa aatcatccac accataatgg ttggggatag
    25381 gcagcgactt gctgacttca tcttccacca gccacattcc ggcaaggccg ttatagacct
    25441 gctgagccgt gcggttggga gtattggcgt gataccacag agtagctgcg ttctggcgaa
    25501 taggcagtac gggtgcccag tcagcgtttg gcgacatcat ccgtgccgga ccgcccatca
    25561 gcgggccagg tacctgtagc ccggccaccg tcattgagac attttctgtc aggcggttgc
    25621 tgtaaattag cttaacatcg ttgcccttcc agacgcggat agtcggcccc aggtaacgac
    25681 cattgattcc ccaaaccgac gcgcgtgtcc ctggcgtaaa tgaccagtgc gcacgttgta
    25741 cagtcataaa cagcggttgc ccacggcgag attcaagcag cggcggaacg ggtagcggtt
    25801 gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc tgcacaaagt gcaatccccg
    25861 atgcctgaat gaactgacgc cgactgagtg acatatttgc tccatgtaaa actggctaaa
    25921 gtaataccgt gcaggaaagt gttccccgca aaaatacaga ttgccgactt aaacttttcc
    25981 ggcggcttcg cgctctgcga cttctttatc gagatcagcg attttttgtt ccattatcga
    26041 acgacaatgg gcagccaact cgcgaacctg atctttgcca tactgactga cgtcaattgg
    26101 cggcagcatt tcgacaatca ccagaccgtt gtgtagccga ttaagattaa tcttattcga
    26161 agttgtagag acgcacacgg gaataatcgg gacgcccgcc gcaattgccg cgtgaaatgc
    26221 cccggtcttg aacggtagca ggccgcgacc acggctgcgg gttccttccg ggaacatcca
    26281 gatggaaata cggcgttttt tgaagtgatt cacgacttcc gcaatagtgc cgtgagcttt
    26341 agtgcgattg tttctgtcga tcaataggtt gccggttaac cagtacaact gcccgaagaa
    26401 ggggatccac agcaagctct ttttccccac cgtcaccgtc ggcggttgca caatgttcga
    26461 tgctgtcacc atgtcatagt tgttctggtg gttagcgata taaatagcat tgccgtagct
    26521 ttcagcatct gcaggtttgc ggcactcaac tttcaggccg aacagcggcg caagacgacc
    26581 aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc gggctgaaaa ggcagtaaat
    26641 ggagccgaat acacagacta agatgctgta aatcacggta ataataagac gaaagatata
    26701 tagcataaca ccctctgaag gtctgagaga ctggcattgt acgtcacctg aaatcaggtt
    26761 agggcattgt taaaagccgc tctctggaaa agcgtattgt taatcgcaac gcatgaataa
    26821 acaacggttt caagggaatt tttatcgttt ccgggggttg aatacgctgc cggatgacga
    26881 cttaacgttt catccggcgt tccttgcaag cgggaggaaa cagcgccctc cccggcatat
    26941 tactcttcgc tatcaccgct gctggcacgg cgaggagagt cgatctcaac acgatcgata
    27001 cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt cgccagtgac tttctgtagc
    27061 tcttccggac gcagtttaat tttgcgtttc ccaacatgaa tggtcagcgt gctttgcggc
    27121 ggcagaacgt acagttgcgc cagaccatct tctccacgcg cggcttctgc cgatggaatg
    27181 ttgataatct tgttgccttt gcccttcgac agctgcggca gatcgcttac cgggaacatc
    27241 aacatacggc ctgcctgagt gattgccagc agcatatcgg aagcatcctc aatcaccacc
    27301 ggcggcataa catgggcatt ttccggtaag gtgatcaaag ctttacctgc acggttacgc
    27361 gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg catcggaagc catcagcagt
    27421 ttctgatcgt cgctttccat cagcatatgg tcaacggtcg ccccaggcgg caacgttaat
    27481 ttgccggtga gcggttcgcc ctgaccacgc gccgacggca gcgtaatcgg gtcaatggca
    27541 tagctacgac cggtggaatc aacaaacact accggttggt tgctcttacc tttcaccgcc
    27601 gctttgaagc tatcgcccgc tttataattc aggcccggcg cgtcgatatc atggccttta
    27661 gcgctgcgta cccagcccat ctgcgacagc acaatggtga caggttcaga cggcagcatg
    27721 tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca acggcgaacg acgttcgtca
    27781 ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca ggttattcat tttacgctcg
    27841 gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca gctcactctg ctcaccgcga
    27901 atcttcatct cttccagttt ggcaagatga cgcagtttca gttcgaggat cgcttccgcc
    27961 tgggtttccg taaggccaaa ccgcgacatc agcgccggtt tcggttcatc ttcattacga
    28021 atgatctcaa tcacttcgtc gatattgaga aacgccacca gcaaaccttc gaggatatgc
    28081 aggcgcttga ggaccttctc caggcgatag ttcagacggc ggcgtacggt atcacgacgg
    28141 aacaccagcc attcagagag gatttcccgc agatttttca ccgccggacg cccatccaga
    28201 ccgatcatat tgaggttaat acgatagctc ttttccagat cggtggtggc gaagaggtga
    28261 ttcatcacct gctccatatc cacgcgattg gaacgcggca cgatcaccag acgggtcggg
    28321 ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg gcagcttctt gttgcgcatt
    28381 tgcgcagcaa tctgctccag cacgcgcgcg ccagaaacct gatgcggtag tgcgctgata
    28441 accaccgcgc catcttcttt cttccacacc gcgcgcatac gcacagaacc gcgaccgttc
    28501 tcgtagattt tgcggatctc cgctcgggag gtaatgattt ccgcttcggt cgggtagtct
    28561 ggcccctgca cgatatccag taactggtcg agcgtggtat tgggctgctc aattaaggcg
    28621 attgccgcct gcgcgacttc acgcaggttg tgcggcggaa tatccgtcgc catgccgacg
    28681 gcaatgccgg tggtgccgtt aagtaaaata ttcggcaagc gagcggggag catcttcggc
    28741 tcttgcaacg tgccgtcgaa gtttggcacc caatctgccg ttccttgccc cagttcactt
    28801 aataaaagct cggaatattt cgacaggcgg gattcggtgt aacgcattgc cgcgaacgat
    28861 ttcggatcgt ccggtgcgcc ccagttcccc tgcccgtcca ccagcggata acgataagag
    28921 aatggctgcg ccatcagcac catcgcttca tagcaggcga tgtcgccatg cggatggtat
    28981 ttacccagta cgtcaccgac agtacgagcc gactttttaa atttggcggt agcattcagg
    29041 cccagttctg acatcgcata cacaatgcgg cgttgaacgg gtttcagacc atcaccaata
    29101 aacggcaacg cacggtccat gatcacgtac atggagtagt ttaagtaggc gttctccgta
    29161 aactcatgca gcgcaaggcg ctctgccata tcgctcatta attctgattc ctcaacttat
    29221 tcgccagcct tcaaaggggc aatattgctg gagatactac attatctgcc gcgtcgagtc
    29281 acaggagatc agcgtcatta gcgccgccgg acgtgctccg gcggcgataa aggcatcaat
    29341 gtgccttgat atacaactct ttgctgtaga cttgatcggc atagggggca acacaaggtt
    29401 gcgcccatgc cacaccactc ggcatgcggg tccgcaccgg gcgattcgag aagttgaggt
    29461 tatgcgagtc cagggaagcc taaagcatca aagtagtcgt ctgtaagtat acggttcagc
    29521 ccagcttagc agtgtctcca cctgtgcccc gcgcctcctg ccataagtgc ggcctgctca
    29581 tgggtaccct cgagacggct tacggcgcac cggataataa atttcgcgta tacatggcat
    29641 tccttaccgg agaacttata agcccgacaa caatacgcct gtggcattgt tcgctcaaac
    29701 ttatcgcgag tgatttggct cacactccgg taaagattct tcatcacgcc aacggcacat
    29761 aaacatccgt ttccagtctc tgctctgtca tcccctcggc aagattggtg tagtgaaaca
    29821 gaatcggtgc tttacgcatt ttctcgccgc ttgcaggcag ccagtggcga atgatgcccc
    29881 ataccgtgtg tgaaatatcg tccagctcgc caacatggcg ggccacagca taacgtccac
    29941 cggtaagttc accattactc acgccataac gattatcggg aatcggttcg ctaacgctgc
    30001 cgcagatatc aaagcgaaac gcttccggtg cggtggtggc aggatcatcc caggcgacgc
    30061 caaaagtctg gctttggtta acaggagaaa gccccgtctc tttacgccac atgataaatt
    30121 tcgccacgct gtagttgagc aactcagggc tacagcggtg cggcaacatc gccacaggga
    30181 ttgatgggaa atcgataatg tttacatcca gtgtcaggtt tgtcattttg cctccctgcg
    30241 aaattattcc cgggaacgcg tcccgggagc ggtaacaatt acggatttac tttgcggatc
    30301 tgtttgacgt caatttcaac agagttccag tctttatcga cttccccctg gatctcaacc
    30361 gtatctttcg gcgtcaccgt cacgccgttc cagcgtttgt ggtcgatatc aacattgata
    30421 gtaccgctgg catctttgaa cacgtagaga tcgtcagaga tgcgttcaac gatattgccg
    30481 cgcagggtta cccaggtgtc gtcacgcagg gattttgcgc tttctacggt cgttacgctg
    30541 ccgttcggcc cctggaatcc tccggcctga ctttgcgttg ccgatgggcc agaaaaaccg
    30601 ccctgctctg ctgccatcac cggtgcgctg cacagggcca ttactgcgat tactgctgcg
    30661 aattttttca tgtttattac tccctttaat gtctgtttcc gagcatttaa caagatagtc
    30721 cttaacaact tcttaaggga aaaaaataaa atttaatgct gtacagagcg cgttacaaca
    30781 cggtttactg gcagcaaata cggttatcgc agggatgaaa aaatgcgaat tttactgata
    30841 gaagatgaca tgctgattgg cgacggcatc aaaacgggcc ttagtaaaat gggttttagc
    30901 gtcgactggt ttacacaagg tcgtcaggga aaagaggcgc tatatagtgc gccttatgat
    30961 gcggtgatcc tggatttaac cttaccaggc atggatggtc gcgatatttt gcgcgaatgg
    31021 cgagaaaaag gtcagcgtga gccggtactg atcctgaccg cgcgcgatgc gttagcggaa
    31081 cgtgtagagg ggctgcgtct gggagctgac gattatctgt gtaaaccttt tgcgttgata
    31141 gaagtcgccg ccaggctgga agctctgatg cgccgaacca acggctaggc cagcaacgaa
    31201 ctgcgccacg gtaacgtcat gctcgacccc ggcaaacgta tcgccacgct ggctggcgaa
    31261 cccttaactc tgaaaccaaa agaatttgcc ctgctggaat tactgatgcg taacgctggt
    31321 cgggtactgc cgcgcaaact gattgaagag aaactgtata cctgggacga agaggtcacc
    31381 agtaatgccg ttgaagtgca tgtgcatcat ctgcgacgca aactcggcag tgattttatt
    31441 cgtaccgttc atggtattgg ctacacatta ggtgagaaat gaaatttacc caacgtctta
    31501 gtctgcgagt caggctgacg ctaatctttt taattctggc ctcagtgacc tggctgcttt
    31561 ccagctttgt cgcctggaaa caaacaacgg ataacgtcga tgagttgttc gacacccaac
    31621 tgatgctgtt tgccaagcgg ttaagtacgc tcgatctcaa cgaaatcaac gcggcagatc
    31681 gcatggcaca gacgccaaat aaattaaaac acggtcatgt tgatgacgat gcgctgacct
    31741 ttgccatctt tacccacgac ggcagaatgg tccttaacga tggcgataac ggagaagata
    31801 ttccctatag ctatcaacgg gaaggttttg ctgacgggca actggtcggt gaagacgatc
    31861 cctggcgttt tgtctggatg acctcacctg atggcaaata tcgcatcgtt gttggtcagg
    31921 aatgggaata ccgcgaagac atggcgctgg cgattgttgc cgggcaattg atcccgtggc
    31981 ttgtcgcact gccgattatg ttaatcatca tgatggtact actgggtcgt gaactcgcgc
    32041 cgctgaacaa actggcgctg gcactacgta tgcgtgaccc tgactcggaa aaaccactaa
    32101 acgcgacggg cgtacccagc gaagtgcgtc cactggttga gtcgttgaat caactgttcg
    32161 cccgtacaca tgcgatgatg gttcgtgaac gacgctttac ctccgacgca gctcacgaac
    32221 ttcgtagccc gttaacggcg ctgaaagtgc aaaccgaagt tgcgcagctc tctgacgatg
    32281 atccgcaggc gcggaaaaaa gcgctgcttc aattacattc cggaatcgat cgcgctactc
    32341 gtctggttga tcaactgctc acgctatcgc ggctggactc actggataac cttcaggacg
    32401 tcgcggagat cccgcttgaa gatctcctgc aatcgtcggt gatggatatt taccacacgg
    32461 cgcagcaggc gaaaattgac gtgcgactga cactcaatgc ccacggcatc aaacgcaccg
    32521 gacaaccgct gttgctaagt ttgttggtac gaaaccttct ggataacgcc gtgcgttaca
    32581 gtccacaggg cagcgtggtg gacatcacgc tgaatgccga tagtttcatc gtgagggata
    32641 acggccccgg tgtgacgcca gaggcactgg cgcgaattgg cgaacgcttc tatcgcccac
    32701 ccggacaaac cgctaccggc agtggacttg ggctatcgat tgtccagcga atcgccaagc
    32761 tgcatggcat gaatgttgag tttgggaatg tggaacaagg tggatttgag acgaaggtaa
    32821 gctggtaagg gtaagatttt tgctaaattc gcaaaagact ttgcacattt tgctaatttc
    32881 accgtaccgc tctgtgacgt actatagtcg acaaacgtct caccttgagg ttaaaaaatg
    32941 agcaacaccc tgattatcaa cggcgcgaaa aaattcgccc actccaatgg tcaactgaac
    33001 gacaccctga ccgaagtcgc ggatggcaca ctgcgcgacc tcgggcatga tgttcgcatc
    33061 gttcgcgccg acagcgacta cgatgtcaaa gcggaagtac aaaactttct ctgggctgat
    33121 gtggtgatct ggcagatgcc aggctggtgg atgggcgcgc cgtggacagt gaaaaaatac
    33181 attgatgatg tattcaccga aggtcacggg actctgtatg ccagcgatgg tcgtacccgc
    33241 aaagatccgt cgaaaaaata cggttccggc ggcctggtac agggcaaaaa atatatgctt
    33301 tctctgacct ggaacgcacc aatggaagcc ttcaccgaaa aagatcagtt cttccacggc
    33361 gttggcgttg acggtgtgta tctgccgttc cataaagcaa accaattcct tggcatggaa
    33421 ccgctgccga catttatcgc taatgacgtg ataaaaatgc ctgatgttcc ccgctatact
    33481 gaagaatatc gcaagcatct tgtggaaatt tttggttaac tagagctcag gctttagaag
    33541 gagttaacca tgcttaccgt aatcgcagaa atccgtactc gtcctggtca acatcaccgt
    33601 caggcggtat tggatcagtt tgctaaaatc gttccaaccg tactgaaaga agaaggttgc
    33661 cacggctatg cgccaatggt ggattgcgct gctggcgtga gtttccagtc tatggcaccg
    33721 gattctatcg tgatgattga gcagtgggaa agcatcgcgc atcttgaagc gcacttgcag
    33781 accccgcaca tgaaagcgta cagcgaggcc gtaaaaggcg atgtgctgga gatgaatatc
    33841 cgcatcctgc aaccggggat ttaacattcc tgaaaagagg ccggtgttgc taccggcctc
    33901 aggtcagatt agccattctt tcttcccgtt ttcagtcctc gatggaaatc gttaattcgc
    33961 ttaatggttt taaccgcacg ctgcggcact gcggaagcta ccgaccagac aatcatttcc
    34021 agacacaaca ataccgtacc atgtagcggc gtttttcctt tttcatcccc gcgcggaaca
    34081 tgaatcacga tactggcgtc tttgctaaaa cgtgaatcca gcgcgtttgt cagcaaaata
    34141 acagggatcc ccagccgtct cgcttcacgc agcgttgtca gtccttcccg gtgcgcagat
    34201 ttttgcgcca tcatgatcag aacatcgcca cgttgaagcg cgatcagttg ctcggcaagg
    34261 ccaatacccg tacggtttag tgccgtggca ggcaaaccta tgcggttgaa tagtcgtgcg
    34321 gtatagtccg ccaggatacc cgaagcgcca atgccaaaaa tagcgacttg ccgtgcctgc
    34381 accaacaacg ctacagcttg cgcaagcgcg tggcggtttt caggctctga caggacatta
    34441 caagtataca aatgcccttc aagaacaaag tcgattgcgg tgttaacatc gctcgtcaag
    34501 ttactcaccg tcgtagacat cttttcgctg gaactaaggg ctggaccaag ccactgttcc
    34561 agagtgcgtt tcagatcacg cagcccggca aaccccagag cctgtatagc cctgataact
    34621 gtggcatcgg aagtgttcaa cgtggccgca atttccatcg ccgtttgctc gagtaccact
    34681 tcacgatgct cattaatgta gcttgcgaca gttcttaacc caggcgttaa ctgactcgcg
    34741 cgagtgcgaa aacgctcacc ataaatatca acccgcgttt tgtctttctg gatcatttag
    34801 cctccggtag aggtcgtccc gcaatttgtg attgtatctg cgcagccatc agccccagag
    34861 atgcaaagga gttagaaatc gcctcttcac gagaaatcag cacatagcgc ccggaacgac
    34921 aggccgtcag aaactgacac catcccggca tgactttttc cattgctgcc agctcatcct
    34981 gaggtttagc accggtgtca ccgcgccagg ttgcaaaaac aaaatcggca tccagttcag
    35041 gtaaacgctc tgcgctcaca tccattcgcc caccttcagg aatgctctca atcagcggcg
    35101 gaaagcgaaa accagcatcg cgtaataccc gccccagcga atgatagcta tgcatcacat
    35161 taatttttcc ctgatttgcc tgaatcaccg ataccgttat tttttggctg tccagcgtcg
    35221 ccttcagcgc attgatttgt gcctgatagc ggcgttcaag tatcgccagc tgcgactgcg
    35281 ttcctgtaag ttctgccagc ttgcggtaga tttctggcgc gcctcctttg agatgatcaa
    35341 tgctcaccgt gggcgcaatt ttttccagtt gttcaatggg cgtattacgg gtcggttctg
    35401 tgatgatcag gtcaggcttt gccgccacaa tcgcttcaat atcaatatca gcggtgccaa
    35461 taaaagcgat cgatgaatta tcaaagtcga cacctgtcaa taacgcgcct gatcgaatga
    35521 aatggctacc gtcaggccgg gttcgcccgt ggctggcgac aggaggtaca ccgagctcaa
    35581 tcagaggaat agtgatatca agatcgtgta aagagacaat tctctttgga tgagcaggaa
    35641 cagtgacttt gcgccctaaa tcatcagtaa agacctgaac aggttctgtc gcgcgggcaa
    35701 agaatgacaa cagtatcagc agagaaaaaa ataaacgcat ataactccca ttaaaaactg
    35761 ttcctgcgct gccagagcag tagcaaaaag aatggtccac caatcaatga aataatgatt
    35821 ccggcaggca gttgcaaagg caaaaaagcc aaacgaccaa tgctatccgc caggatgact
    35881 aacaacgccc ccgatacggc gctccccagc agtaatgtcg tctgcccgcc acgtagaata
    35941 aaacgtgata tatggggtgc tatcaatccg acaaaaccga tgttaccaac acacgaaacg
    36001 cagacggctg tcaggattat tggtgcggcg acacgtaaca acgccagacg agaactattc
    36061 acccctaacc cggttgcaac ctgatgacca agcagagcga tatccgccgt cctggcggtg
    36121 aacagcaata aaagagcggc tggcaacatc caacaggctg aaataccgac taacatccag
    36181 ttagctgcat gcaggcttcc cgataaccac atcaatgcag tttgtacatc tcgaacatct
    36241 gcagtggtca taaatacacc gataccggcg gcgaaaaacc aggaaacacc aataccaatc
    36301 agaacaaatc gcgggcggga aatatcgcgc gcacaaaaaa tcacgattaa agccaccaac
    36361 aaaccaccag caagaccagc cactggacgc cagaacattc ccagcattgg aaactggaaa
    36421 atcagccaca gcaccgccac gctacatcct tctttgacgc cgattaaacc aggatcggca
    36481 aggccgttac gcgcaatgga ttgcatcgcg gcccccgcca tacctaacat ggctccgcat
    36541 agcaatgcca tgatgactct cggcaagcga atatcctgca caatataacg tgcgtctgtg
    36601 cttacgtttt ctggagcaaa caacgctcgt cctatttccg atgccgggac agaaaaagaa
    36661 ccatgcatca gaccgaatat cagtattcca accgcaaaca gaaacagccc accaagcaac
    36721 acctgatttt tagggcgtac cagtgtcgaa aacttaccaa cacgtagtgg acgtagcccg
    36781 acgttcatta tccgactcat ttgaacatcc tcgtcgccat aatgacaaaa acaggcgcac
    36841 caactaacgc cgtcatgaca ccggtcgcta actcatgggg cgtgaaaagc gtccgagcaa
    36901 taatatctgc cagtaataac aaaagcgcgc cgacacaagc tgaaagaggg agcagaacgc
    36961 gcagatccgc tgataccaga cgcctgacaa tctgcggtac tagcagaccg acaaaaccaa
    37021 tggggccagc aatcgatacg gctgcgccac agagcaacgc gatcgcaagc agagtgaatg
    37081 ttcgcgtgcg gagtaccgag acgcccagcc cctgcgccat gcgatcaccc agtgccaaca
    37141 tgttaagaga gggcgcaagg taaatggcta acacaacccc ccccagagaa aaccaggcgg
    37201 acgtacccag cgtcgcccaa tcctgccccg ctaaatcacc tgctagccat gtccgcatcg
    37261 ccagcagtgt ttgttcgtca agaatcagga ttgcagccgt tattgaggac acgaacgccg
    37321 atagcgccac gccgcataaa gtaactttca ttggcgttaa ccctgaacgc cctgcggagg
    37381 aaagcagcaa tatcaacagg aacaataacg cgccgcccgt agatgccagc agtgggcgtc
    37441 caaccggaaa ggccagccca agcgcactgg cagcaacgac agcaagagcg gctccggcat
    37501 ttaagcctaa aatatgtggc tcccccaatg ggttacgaat gacagcctgc aacaaagcac
    37561 cagcaactcc aagagaagca ccggtaagca atgctgccgc taaacgcggc aaacggagtc
    37621 ttacaattac gttgtgatcg aagttttgcg gttcaaaatg aagaaatgcc tgcaacaccg
    37681 tttgtggtgc aataaatcgg gcaccaatcc ctggatatac cacgcatccc accagcagta
    37741 aggcgaggaa aattacagta accagccctg tgcgcatcat actttttcta caccacgaaa
    37801 cggcatgaag aacggctttc ccgtcagagg atttattgat gcgtgaacat caacgtcaaa
    37861 gacagctttt accagctcag gcgtgcaatg ttccccctca ttcaacacac gcaccacttt
    37921 cccctggcgt aaaaatatca acgtatcacc ataattgacg gcaaaattca gatcatgcaa
    37981 caccacgacg actgtacgcc cgtgatgacg cgttaaatca tgtaataact caagaatttc
    38041 aacctgataa cgtaaatcaa gccaggttgt gggttcatcc agcaggatat aaggcgtctt
    38101 ttgcgccaac accatcgcaa tccagcaacg ctggcgctgc ccaccagaaa gtttttcaac
    38161 aggtaaatgt gcaaactcct gcgtaccggt taatttcagg gcctcttcta ccgcctgctc
    38221 gtcggcgtcg ctccattgac gaatgaaatt ctgccaggga aaacgcccac gcgacaccag
    38281 ttcataaacg gttaagccct ccggcaataa tggcgactgc ggcaagatcc caagtctacg
    38341 ggataacgct ttcgttggtt gctcgtgaat cgctttacca tccagcaata tcgagccgcc
    38401 cataggttgc aagatacgtg caatggtgct gagcaacgtc gactttccac agccgtttgc
    38461 cccaaccaga acggtcattt tttctgtggg aacggagaaa gaaacaccat caacaatgat
    38521 tttcgtgtga tacccggcag aaacattgtc gaggatcagc ccctgccctt ttgcgttatg
    38581 tgccacgtgt acgccaactt aatcaaagac aaacaaaaat aaaactcatt ctcctttgag
    38641 agtttgacgc cacaaaatgt agtagtcaag aagttaaaag ccatacaaaa acaaggt
//
LOCUS       DAOTRK010000025        38495 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.025, whole genome shotgun sequence.
ACCESSION   DAOTRK010000025 DAOTRK010000000
VERSION     DAOTRK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.025"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(230..421)
                     /locus_tag="QIE41_001316"
     CDS             complement(230..421)
                     /locus_tag="QIE41_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDS3530224.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            439..591
                     /locus_tag="QIE41_001317"
     CDS             439..591
                     /locus_tag="QIE41_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530225.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            773..3016
                     /gene="pdeF"
                     /locus_tag="QIE41_001318"
     CDS             773..3016
                     /gene="pdeF"
                     /locus_tag="QIE41_001318"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDS3530226.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(3055..4596)
                     /gene="ppx"
                     /locus_tag="QIE41_001319"
     CDS             complement(3055..4596)
                     /gene="ppx"
                     /locus_tag="QIE41_001319"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDS3530227.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4601..6667)
                     /gene="ppk1"
                     /locus_tag="QIE41_001320"
     CDS             complement(4601..6667)
                     /gene="ppk1"
                     /locus_tag="QIE41_001320"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDS3530228.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6838..7476)
                     /gene="purN"
                     /locus_tag="QIE41_001321"
     CDS             complement(6838..7476)
                     /gene="purN"
                     /locus_tag="QIE41_001321"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDS3530229.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAIVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7476..8513)
                     /gene="purM"
                     /locus_tag="QIE41_001322"
     CDS             complement(7476..8513)
                     /gene="purM"
                     /locus_tag="QIE41_001322"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDS3530230.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8838..9464
                     /gene="upp"
                     /locus_tag="QIE41_001323"
     CDS             8838..9464
                     /gene="upp"
                     /locus_tag="QIE41_001323"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDS3530231.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9550..10839
                     /gene="uraA"
                     /locus_tag="QIE41_001324"
     CDS             9550..10839
                     /gene="uraA"
                     /locus_tag="QIE41_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDS3530232.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10889..11635
                     /gene="hda"
                     /locus_tag="QIE41_001325"
     CDS             10889..11635
                     /gene="hda"
                     /locus_tag="QIE41_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDS3530233.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11741..12100)
                     /gene="arsC"
                     /locus_tag="QIE41_001326"
     CDS             complement(11741..12100)
                     /gene="arsC"
                     /locus_tag="QIE41_001326"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDS3530234.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12121..13584)
                     /gene="bepA"
                     /locus_tag="QIE41_001327"
     CDS             complement(12121..13584)
                     /gene="bepA"
                     /locus_tag="QIE41_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDS3530235.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13797..14858
                     /locus_tag="QIE41_001328"
     CDS             13797..14858
                     /locus_tag="QIE41_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDS3530236.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14896..15744)
                     /locus_tag="QIE41_001329"
     CDS             complement(14896..15744)
                     /locus_tag="QIE41_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDS3530237.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNVMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(15766..17778)
                     /gene="hyfR"
                     /locus_tag="QIE41_001330"
     CDS             complement(15766..17778)
                     /gene="hyfR"
                     /locus_tag="QIE41_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDS3530238.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLAHIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRCMPGESVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSVSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17808..18221)
                     /gene="hyfJ"
                     /locus_tag="QIE41_001331"
     CDS             complement(17808..18221)
                     /gene="hyfJ"
                     /locus_tag="QIE41_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDS3530239.1"
                     /translation="MAEECREIVFWMLRKKFIASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18214..18972)
                     /gene="hyfI"
                     /locus_tag="QIE41_001332"
     CDS             complement(18214..18972)
                     /gene="hyfI"
                     /locus_tag="QIE41_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDS3530240.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18969..19514)
                     /gene="hyfH"
                     /locus_tag="QIE41_001333"
     CDS             complement(18969..19514)
                     /gene="hyfH"
                     /locus_tag="QIE41_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDS3530241.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDYQQNTRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDEPLVAKEQL"
     gene            complement(19524..21239)
                     /gene="hyfG"
                     /locus_tag="QIE41_001334"
     CDS             complement(19524..21239)
                     /gene="hyfG"
                     /locus_tag="QIE41_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDS3530242.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKIVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21229..22809)
                     /gene="hyfF"
                     /locus_tag="QIE41_001335"
     CDS             complement(21229..22809)
                     /gene="hyfF"
                     /locus_tag="QIE41_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDS3530243.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHGLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLLIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHNLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22814..23464)
                     /gene="hyfE"
                     /locus_tag="QIE41_001336"
     CDS             complement(22814..23464)
                     /gene="hyfE"
                     /locus_tag="QIE41_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDS3530244.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFVAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMTLLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23476..24915)
                     /gene="hyfD"
                     /locus_tag="QIE41_001337"
     CDS             complement(23476..24915)
                     /gene="hyfD"
                     /locus_tag="QIE41_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HDS3530245.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGGVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24932..25879)
                     /gene="hyfC"
                     /locus_tag="QIE41_001338"
     CDS             complement(24932..25879)
                     /gene="hyfC"
                     /locus_tag="QIE41_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDS3530246.1"
                     /translation="MRQTLCDGYLVIFALAQAVTLLVLTPLFTGISRQIRARMHSRRG
                     PGFWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFHFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISKTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKLGLGLKQVVMASLFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25890..27908)
                     /gene="hyfB"
                     /locus_tag="QIE41_001339"
     CDS             complement(25890..27908)
                     /gene="hyfB"
                     /locus_tag="QIE41_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HDS3530247.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVMVISLLVVVCSLYSLTY
                     MREYEGKGAAAMSFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAIDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVFIALSASWLAPKIMHIAHAFTN
                     TPPVTVTSGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWEHAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27908..28525)
                     /gene="hyfA"
                     /locus_tag="QIE41_001340"
     CDS             complement(27908..28525)
                     /gene="hyfA"
                     /locus_tag="QIE41_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDS3530248.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQADGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28778..29248)
                     /gene="bcp"
                     /locus_tag="QIE41_001341"
     CDS             complement(28778..29248)
                     /gene="bcp"
                     /locus_tag="QIE41_001341"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDS3530249.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29248..29820)
                     /locus_tag="QIE41_001342"
     CDS             complement(29248..29820)
                     /locus_tag="QIE41_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDS3530250.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29966..30844
                     /gene="dapA"
                     /locus_tag="QIE41_001343"
     CDS             29966..30844
                     /gene="dapA"
                     /locus_tag="QIE41_001343"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDS3530251.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30861..31895
                     /gene="bamC"
                     /locus_tag="QIE41_001344"
     CDS             30861..31895
                     /gene="bamC"
                     /locus_tag="QIE41_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDS3530252.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32108..32821
                     /gene="purC"
                     /locus_tag="QIE41_001345"
     CDS             32108..32821
                     /gene="purC"
                     /locus_tag="QIE41_001345"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDS3530253.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32989..33852
                     /locus_tag="QIE41_001346"
     CDS             32989..33852
                     /locus_tag="QIE41_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDS3530254.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33867..35882
                     /gene="tmcA"
                     /locus_tag="QIE41_001347"
     CDS             33867..35882
                     /gene="tmcA"
                     /locus_tag="QIE41_001347"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDS3530255.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPVAENHCFPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQQFSL
                     AHFAPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVIAAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIVGALQYTHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35956..36654
                     /gene="ypfH"
                     /locus_tag="QIE41_001348"
     CDS             35956..36654
                     /gene="ypfH"
                     /locus_tag="QIE41_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDS3530256.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGNDDVIEMM"
     gene            complement(36735..36935)
                     /locus_tag="QIE41_001349"
     CDS             complement(36735..36935)
                     /locus_tag="QIE41_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDS3530257.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36963..38090)
                     /gene="dapE"
                     /locus_tag="QIE41_001350"
     CDS             complement(36963..38090)
                     /gene="dapE"
                     /locus_tag="QIE41_001350"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDS3530258.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHAGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVEWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38094..38450)
                     /locus_tag="QIE41_001351"
     CDS             complement(38094..38450)
                     /locus_tag="QIE41_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDS3530259.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKIIDAASAAALMTEMPAIIKRPLLCTPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9391 a         9918 c        10123 g         9063 t
ORIGIN      
        1 atagattaaa tttattaaaa ttcatgtggg aaataattta taaaaataca tcattaaaat
       61 atatgtatta attttatatt tatttcgcag ccaaacagga taaacatctg tcaggacaaa
      121 gacgtaaaat ttaagatgaa agaatatctg ccgctttgat aatcccaaag agcaaaagca
      181 tccgacagcg aggtgccgtt atgttctgtg aaagtggaga aggtaaccgc taacgttgta
      241 ccggtgattc gacggagagt gtggattgct gagcttcttt tttgctctga tggtgctgcc
      301 atgcaccgac ggacgagtag ataaaacggc caaagaagaa gataaagctg atgagcagta
      361 cgatacgggt catgcgactg ttaaatcggt gtcgttttcg catactggtt gcctgactca
      421 caaaaggttc cttgaagtat gtcccacgct gtggacggta cttacattaa ggcacaacag
      481 gacaaaatgg tcaattcttc gttatgtaaa aaagcgtcag tggatacata ttttaatgtt
      541 atggaagtta atttaattat ttacaatgat gatgtaataa tgatgaatta acatgataaa
      601 cgtaataatt cattaatcag ggttattttg tttgatatat atcaattgga actttcatat
      661 gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag tcaggtggga
      721 tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat ctatgaaact
      781 gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt tcctgccttc
      841 tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt ccggtgaggt
      901 ttttctcttt tatctgcctc tggctctgat gatcagcatg atgatgtttt tcagctgggc
      961 ggcattgcca gggatcgcct tagggatttt tgtccgcaaa tatgcagagc tgggttttta
     1021 cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt ggggcggtta
     1081 cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt taatttccca
     1141 gcgtatattc tggcagattg tgtttcctgc aacgctgttt ctgatacttt tccagtttgc
     1201 tgcgtttgta ggattactgg cgagcagaga aaatctggtc ggcgtcatgc cttttaacct
     1261 cgggacctta atcaattatc aggccttact ggtgggcaat ctgattggtg tcccgctgtg
     1321 ctacttcatc attcgtgtag tgcgaaatcc gttttattta cgtagttatt attcgcaatt
     1381 aaaacagcag gttgatgtca aagtcaccaa aaaagagttc gcactctggc tactggcatt
     1441 aggtgcgttg ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac
     1501 caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagccatgc gctatggtta
     1561 taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca gctatcaaaa
     1621 ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct ccagttatct
     1681 ggtattctct tttattgtca attatatggc tgtactggca acccgtcaga gagcggtagt
     1741 cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa atgttcgcgc
     1801 cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac gcatccctgg
     1861 catggaaatg ctggttaaaa actatggcat catgctacgg attcaataca agcaaaaact
     1921 ttctcactgg ctgtcaccat tgctggaacc gggtgaagat gtttatcagc tatcgggtaa
     1981 cgatctcgcg ctgcgactga atacagaatc gcaccaggag cgcattaccg cactggatag
     2041 ccatctcaag caatttcgtt tcttttggga tggaatgccg atgcaaccgc agattggcgt
     2101 cagttactgc tatgtgcgct cgccagtgaa tcatatctac ttactgctgg gagagctaaa
     2161 tacggtcgcc gaactttcca tcgtgaccaa cgccccagaa aatatgcagc gtcgcggggc
     2221 aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcggc tacagcaggc
     2281 gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc gtggcgatgt
     2341 ctaccatgaa attcttctgc gtatgaaagg tgagaatgat gaactgatca gccccgacag
     2401 cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt gggtcattga
     2461 gcatacgctg caatttatgg ctgaaaacag agcgaagatg cccgctcacc gttttgcgat
     2521 taatctgtcg ccaacctcgg tatgtcaggc tcgttttccc gttgaagtca gtcagctgct
     2581 ggctaaatat caaattgaag cgtggcaact tatttttgaa gtcaccgaaa gtaatgctct
     2641 gacaaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag gctgccagat
     2701 tgcgattgat gatttcggca ccggctatgc cagctatgcg cggcttaaaa atgtgaatgc
     2761 cgatttgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata gtctggatta
     2821 tcagatagtg gcgtcgattt gccacctggc gcgaatgaag aaaatgcggg tagtggcaga
     2881 gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct ttggggatcg attatatgca
     2941 gggttatctt attggtaagc cgcaaccgtt aattgatacg ctgaatgaaa tcgaacccat
     3001 tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa agcattaagc
     3061 ggcgatttct ggggtacttt cttcttcaat tttcaaccgc cagccagcca cgccttccca
     3121 gtattcttgc tccttttcca gatcaagcag taccagcgca ttctgactaa accagtcatg
     3181 cgggaaacgc agtgtccagt gactgtcatc ggtaatcagc gtcaatgttg gcggtgtagt
     3241 ggttgcctga cgttgattat tgagtaatac gccaaggcgc aatagctgaa tcagtggcag
     3301 gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagcttaa tcgctttacg
     3361 gtgatagcgc accagcgtcg ccatcatcaa ctgctgttcc tgattaaaac ccggcaagtc
     3421 gctgttttgc agaatataag ctgagtggcg atgcaaaccg ctgtggttga tattcaaccc
     3481 gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat gcgccagctt
     3541 cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagta cccgtcgggc
     3601 ctgttcgctg tcgatgtgat actggttggc gaggctgctg gcggtgcgac tacgcacatc
     3661 ctgatgacgg aaacgtcctt ccatttcata cagtacgcct tcgcgtaacg ccccgtcaga
     3721 aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg cataaaatcg ccagtcccgg
     3781 aacgaagact gttttccgct cttcggaaag acccggtaaa ctcagcgatg cgaaattacg
     3841 gtgacgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta tcccgtcttt
     3901 ctcgcccatt tccattaaca cttcatgggc ggcttttatg gtaccggaag cgcccattgc
     3961 cacgttccag ccctgaatac ggaattgcca ggttaaagtt tccagttttt gtgctgccgc
     4021 catgcgagcg cgctgaaaat tctctttatt gatgaccccg ccagggaagt aaagctgggc
     4081 aaagctgaca caacccatcc ggcggctttc aacgagaatc ggttcgaaat tttcaccaat
     4141 caccagttcc gtagatccgc cgccaatatc aataaccagt ttgcgacctt tctccggttg
     4201 ggtatgttcc acgcccataa aaatcagacg ggcttcttca ttaccggaaa taatttcaat
     4261 cgggtaggga atgacctttt ccgcgcgttt cagaaagtca gtggcgttca gcgcctgacg
     4321 cagtgtatgg gtgccaacta tacagacgct ggcaggagaa aacccttgca gccgttcggc
     4381 aaacagcgac agacagttca aaccgcgcgt cattgcctct tcactcaaca tattatctgg
     4441 ccccaggccg tccgccagat gcacccgctg tttcaggcgg ccaataatct gcatggcacc
     4501 atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga ccgcagcaaa
     4561 ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt tgttcgagtg
     4621 atttgatgta gtcgtaaatc gccaactgcg cccgtacttt gcggcgattg ccgcggggaa
     4681 cgtagcgatt actgagttct ttatcgatat aacgtgcttt gactgtatcg ctgaacaata
     4741 tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagtggc gtcgccactt
     4801 caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag acctttttat
     4861 cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca acaatactga
     4921 tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac atgccgcgaa
     4981 ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca accaggcctt
     5041 tatcgacaag gttatttagc ttcagggtga taccactggg cagcccttgc tgcgcgttgg
     5101 cgatctcgcg gtccaccatt tcatacaaca ggcggcggga gttttgcggc gacaccatta
     5161 aataatcaaa tgtcaccgga cggtatgggt tttcaataaa gttgaacacc cgccgtactt
     5221 cgttggtgat gcgcgcatcg gcggtcagca acgaatagtc agtataaaga cgcgcggttt
     5281 tttcgttaaa gttcccggtc ccgatgtgtg cgtaacgcac cacttcaccg ttttctttac
     5341 gtgaaatcag gaacagtttg gcgtgaattt tcagccccgg cgcagagaag ataacgtgca
     5401 cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttggc ttcttcgtcg aaacgcgcct
     5461 gtaactcaac caccacggtg actttcttac cgttgtgtgc ggcgtggatc atcgagtcga
     5521 tgatgcgtga atctttcgcc acgcggtaaa tgttaatttt aatcgccagt acgctcgggt
     5581 cgaacgaagc ctgacgcagc agttccagca catgctcaaa ggtatgataa ggataataga
     5641 gcaacacatc gcgttcgcga atggcatcaa aaccattgcg gaactgggct ttatcaaacc
     5701 aaatgtggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg acattgggga
     5761 aattaataaa gtctttaaaa ttatgataac gaccgccggg gacgatggag tcgtagcggg
     5821 aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg cgctgataaa
     5881 caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatc aactccatca
     5941 ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc ttcattgaat
     6001 aggcattcag tgcgtcataa tcaaagaagc ctttgaaaat atcatcaagg cagtaacgca
     6061 gaatgttatc cagaagaatc atcggcttgc gtcgacgcgg cgcttctggc ggtaaattca
     6121 caaagcgcgg cactttatct gatgggatct ccagcagcgc gtaacggatg gtatcgccac
     6181 ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact aagtcagtgt
     6241 caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa taatgacgca
     6301 gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc tggttgcgcg
     6361 ccatctccag cagtaactcg ttgtagaggc cgtcgaattc ctgatcggct ttcagcaccc
     6421 gggactgaat tttgcccagt aaatggcggg aatgagagtt ggagccttgt tcttcgctaa
     6481 taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca aggttattgg
     6541 aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc gccgcttcct
     6601 gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat agcttttcct
     6661 gacccattac cacttttact ccgttttatt cacgggacac tgcctggata ttatggcgag
     6721 caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct ggagcttgcg
     6781 atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta cggggcatta
     6841 ctcgtcggca gcgtagccct gcggcggcag acgttgacca tccagccacg cggcgttttc
     6901 gtgcattttc agacgaccat cggcaaacca gctaatcacc agtggataaa tagcgtgttc
     6961 ctgggtttgc acgcgggcgg tgatgtcatc ctccgtatca ccagcaaata ccgggacttt
     7021 cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg acgaaatgca ccgatgtacc
     7081 gtgctcttca tcgccatttt ccagcgcctg acgatgggtg tgtaatccgg gatatttcgg
     7141 cagcagagaa gggtgaatgt tcagcaaacg cccggcatag tgggagacaa tcgccgggct
     7201 gagaatgcgc ataaaaccag ccagtacgac cacatcgggt gcgtacatgt cgatttcatg
     7261 aatcaactcc cggtcatagg cttcacgact gtcaaacgcg ctggcgatga gcgtatgcgt
     7321 tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac
     7381 tgcccgtacg gtgcctttaa ttttattggt tttgcaggcg tcaataattg cctgcaaatt
     7441 acttccgttg ccggaaataa gcaccacaat attcattatt cgataaccac gcgttgttcg
     7501 gaatcagagg ctttgatgat accgattttc cacgcgtttt caccgttggc attgagcagg
     7561 gcgagggctt tgtccacttc cggagcaggc agggcaataa tcatcccgac gccgcagttg
     7621 aaggtgcgat acatttcatg gcgctcaacg ttacctgccg tttgcagcca gttgaacact
     7681 tccggccact gccaggatga ttcatcaatc actgcctgag tattatctgg caatacgcgc
     7741 ggaatgtttt cccagaagcc gccgccggtc aggtgcgcaa tagcatgcac atcgaccttt
     7801 tcaatcaact ccagcacgga cttcacgtaa atgcgggtcg gtgccagcag atgatcggct
     7861 aatggcttac catcaagttc ggtggtttgc ggatcacaac cgctgacttc aagaattttg
     7921 cgcaccagcg aatagccgtt cgagtgcgga ccgctggagc cgagtgcaat cagcacatcg
     7981 ccatcgctga ctttagagcc gtcgatgatt tctgattttt ctaccacgcc aacgcagaaa
     8041 ccagcgacat cgtaatcttc accgtgatac atccccggca tttctgccgt ttcgccaccc
     8101 accagtgaac agcccgattg cagacaacct tccgcgatgc cgctgatcac cgctgaagcg
     8161 gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg ttctgcacct
     8221 tgcaccacca ggtcattaac gcacatggcg accagatcaa taccaatggt gtcgtgacgt
     8281 tttaagtcca tcgccagacg cagcttggta cctacgccgt cagtgccgga aaccagcacg
     8341 ggttcacgat atttttgcgg caatgcacac agcgcaccga agccgcccag accgcccatc
     8401 acttccggac gacgcgtttt cttcactacg cctttgattc ttccaaccag agcattaccc
     8461 gcgtcaatat caacaccggc atctttgtag ctaagagagg ttttatcggt cactgcttgg
     8521 gtccccacgc gttacttgcg gtagaaaaat aaaattcggc gcaattctaa cagggaaagc
     8581 aaacgtttgc gagactgctt tacacaacct ttttgcacgt cttttcccca ggcgcgcggc
     8641 gaaagaatac ttgtaccagg gcaaaggatc gttttcggat ggaataatct tcttctataa
     8701 ccatttgaat ataaaataac tttatctcaa accgttatca ttttgactaa agtcaacgaa
     8761 aataatattg ccgccttgaa gaaaggaggt ataatccgtc gatttttttt gtggctgccc
     8821 ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc aaacacaagc
     8881 tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc gcttccgaag
     8941 tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa gtaactatcg
     9001 aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt accgttgtgc
     9061 caattctgcg tgcgggtctg gggatgatgg acggtgtgct ggaaaacgtt ccgagcgcgc
     9121 gcatcagcgt cgtcggtatg taccgtaatg aagaaacgct ggagccggta ccgtacttcc
     9181 agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgaccca atgctggcaa
     9241 ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa agcgggctgc agcagcatca
     9301 aagttctggt gctggtagct gcgccagaag gtatcgcagc gctggaaaaa gcgcacccgg
     9361 acgtcgaact gtatactgca tcaattgacc agggactgaa cgagcacgga tacattattc
     9421 cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata aaaataatta
     9481 aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca taatacagag
     9541 gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact tttacagaca
     9601 atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc
     9661 ttatttcata ttaacccggc gactgtactg ttatttaacg gtataggaac actgctgtat
     9721 ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc ctttatttca
     9781 ccggtattgt tactgttgcc gttggggtat gaagtcgcgc tgggcggctt tattatgtgc
     9841 ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg ctggctggac
     9901 gtgctgtttc cacctgcggc aatgggcgca atcgttgccg tcatcggtct ggagctggcg
     9961 ggcgtagctg ccggtatggc aggtttactc ccggctgaag ggcaaactcc agactccaaa
    10021 accatcatca tctccattac caccctggcg gttacggttt taggttccgt gctgtttcgt
    10081 ggtttcctgg caattatccc gattttaatt ggcgtgctgg tgggctacgc gctctctttc
    10141 gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc gctgccaact
    10201 ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctgccagc ggcgttagtg
    10261 gttattgccg aacacgtagg gcacctggtg gtaacggcta atatcgtcaa aaaagatctg
    10321 ctgcgcgatc caggtctgca ccgttcgatg tttgctaacg gcttgtcgac cgtgatctcc
    10381 ggcttctttg gctccacgcc aaataccacc tacggggaaa acattggcgt gatggcgatc
    10441 acccgtgttt acagtacctg ggttatcggc ggggcggcga ttttcgctat cctgctttcc
    10501 tgcgtcggta aactggctgc tgctatccag atgatcccat tgccggtgat gggcggcgtt
    10561 tcgctgctgc tttatggtgt catcggtgct tccggtattc gtgttttgat cgaatcgaaa
    10621 gtggattaca acaaagcaca gaacctgatc ctgacttccg tgattttgat catcggtgtc
    10681 agtggcgcga aggtaaacat cggcgcggcg gagctgaaag gtatggcgct ggcgaccatc
    10741 gtcggtatcg gcttaagttt gatcttcaaa ctgattagtg tgctgcgccc tgaagaagtg
    10801 gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg ggcagtgttg
    10861 ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct
    10921 ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc ttcctgacga
    10981 cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggccg cgctgcaaaa
    11041 cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag gcgcggggcg
    11101 cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta
    11161 tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta tggagcattt
    11221 gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt gggagatggc
    11281 gattttcgat ctctacaatc gaattctgga atcgggcaaa acacggttgt taatcaccgg
    11341 cgatcgtcca ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc gtctcgactg
    11401 ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg cgctacagtt
    11461 acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct tgttgaagcg
    11521 gctcgacaga gagatgcgca cgttatttat gacgttggat cagttggatc gtgcgtcgat
    11581 taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaaac tgtagacaag
    11641 acgtttattg tcggatgcgc acccatagta ttcgtcggcc ggataagacg cggcaagcgt
    11701 cgcatccggc aatgtgcttc aactagcgat ggagcacttc ttaacccacg atttccagca
    11761 cctgctccgg tggacgacca attcgcgctt tgccattcgc gaccacaatc gggcgctcca
    11821 ttagcttcgg attatccacc attgcctgaa tcagcgcctc ttcgctaagc gaactgtcag
    11881 ccaggttcag ttctttataa agatcctctt tctggcgcat caattcacga gcgctattca
    11941 tccccagcat tttcagcaaa tcacgcagcg ttgccgcatc ggcgggtgtc tcaaggtaga
    12001 gcaccacttc tggctccacg ccgttttctt tcagcaggtt cagcgtttcc cggctctttg
    12061 aacagcgtgg gttatggtag atttttacct gtttggtcat tgttcttcct ttaatgcgaa
    12121 ttacatcttg gtataaggct taaagcgttc ctgcagctgg cgcaactggt cgatgcgcgc
    12181 atcgtaacgt gcttgttgca ggctgcctaa tttcacctgc gaactggcgc tactcaacaa
    12241 ggaaatagcc tgatcgagtc gcccggcgag cgcataacct tctgctcgcg cagccagttc
    12301 ctgatcgcgg ttatttagcg cggcttccgc ctgtgccagc aaatcccagc cgttgctgtc
    12361 atctttatta ttaaaggtgt agcgattcag aatattggcc gcttcttgtg gttgaccgcc
    12421 ttgcagataa gcgttcgcca ggttgagctg caacaccgga ttggtgcgta aatcacgggc
    12481 atttttcaga cgattgatag cgtcattggc tttgttttgc ccaagatcga tatcagtagc
    12541 cagatcgaga taccatgcgt tgccaggttc tgccgccagt aacggttgca gcgttttacg
    12601 cgcctcgtcg tatttattgg cttccatcgc ctgtaaagca cgaccatatt gtgctgctcg
    12661 ttgctgacga acgtttcctt tcgcccattc atccagcaaa tcactggtga gctggttacg
    12721 tccggaatta tacatcccca gtgtgcgcgc tttcgccaga tagaaatctt ccgacgactg
    12781 caccaccatc ggacgcatct gattagcacg gttgcgggca tctgccagac gactttccgg
    12841 caacgggtga gtcaataaaa tttccggcgg acgcgaggag taacgcgcct gatcgagcaa
    12901 tttttcgagg aaggtcggca tcgcctgcgg atcgaatccc gagcgttgca gcacctgaat
    12961 accaatgcgg tccgcttcct gttcattttg ctgggtgaaa ctgatcatcc cctggcgcgt
    13021 tcccgccagt gtaccggtca gcgcagccat ccccgcctgc ggactggcca tcgccagtaa
    13081 aatagaacct aatgcgccga cccaggtcag cggcgcgctg cgctgctgat cttccatcgc
    13141 tcgcgccagg tgacgttggg tgacgtggga gatttcgtgc gccataactg aagccagttg
    13201 actttcgtta tcggaataac ggaacagggc agagtgcagc accacgttgc cgccaaagaa
    13261 agcaaaggcg ttaatttcgt cgttgttgat cagaaagaaa tgaaacggtg tcttaaccga
    13321 attggcatgc gaaaccagac gcatacccag cgaattaata tattgcgtta acagcgggtc
    13381 attaattaac ggcgcgctgc cgcgtagctg gcggacataa tagtcgccca tctgcatttc
    13441 ctggccaatg gaaagcgtgc ttcctgcgga ggttcccata tccggcaagg tgtctgcgct
    13501 gtcggcaaac gccggggcta cctgaccaat ggtcatagca gcaatgaggg ttgcaaccag
    13561 gttttttttc aactgcctga acataacctc tatcctgtat ttctgaagat agtcatttga
    13621 ccgatactgt gaaatatcgt tcccaccgcg ccgcctttga gtgtaacggc gaagattagc
    13681 ccactcaaat gacttttcag tttcctgaat ccgtcataaa aagcgaagtc ttttttgtga
    13741 catttcgata caattgcgga tcgcaatccc gctcttgagg ttcagggaag gtctttatgc
    13801 tcgaaatgtt gatgcaatgg tatcgccgcc gttttagcga cccggaagcg attgccttgc
    13861 tggttatttt agttgccgga tttggcatta tctttttctt tagtggcctg cttgctccgt
    13921 tgctggtggc tattgtgctg gcctatttgc tggaatggcc aaccgtgcgc ctgcaatcta
    13981 ttggctgctc ccgccgctgg gcgacgtcga ttgtattggt ggttttcgtc ggtatattgc
    14041 tactgatggc gttcgtggta ttgcctatcg cctggcagca gggcatctac ttaattcgcg
    14101 atatgccggg gatgctcaat aagctttctg actttgccgc cacgttgccg cgccgctatc
    14161 cggcgttgat ggatgcgggc attattgatg caatggccga aaatatgcgc agtcggatgc
    14221 tgaccatggg cgattcggtg gtgaaaattt ccctcgcctc gctggtcggt ttgctgacca
    14281 ttgccgtcta tctggtgctg gtaccattga tggtcttctt cctgctgaaa gacaaagagc
    14341 agatgctgaa cgccgttcgc cgggtgctgc cacgtaaccg tggactggca ggacaggtgt
    14401 ggaaggagat gaatcaacaa atcaccaact atatccgcgg caaagtgctg gagatgatcg
    14461 tggtggggat cgccacctgg ctgggattct tactcttcgg gctgaactat tcgctgctgc
    14521 tggcggtgct ggtcggcttc tcggtactta ttccgtacat tggcgcattt gtggtgacca
    14581 ttccggtggt tggcgtggcg ctattccagt ttggtgctgg cacggaattc tggagctgct
    14641 tcgcggtgta tctgatcatt caggcgctgg acggcaacct gttagtacct gtgttgttct
    14701 ccgaagcggt taacctgcat ccgctggtca ttattttatc ggtggtgatc ttcggtggtt
    14761 tgtggggatt ctggggcgta ttcttcgcca ttccgttggc gacgctgatc aaagccgtga
    14821 ttcatgcctg gcccgatggg caaatcgcgc aagaataatc ttcagcccga cgaactgttt
    14881 cgccgggctg attttttagg gttcctgacg taaataaata gcccgataac aaatactcac
    14941 cagcactgca ccgccgataa tattacccag catcaccggg agcagattgg cggtaataaa
    15001 atggctgacc gtcagtgccg gaaaattgtc tgcgctactg tgcgccagtt gccagaaggg
    15061 gggaggggcg aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca
    15121 gtgctcaaag ccacttgcga caaacagggt gatgggcaaa atcatggcga cgattttgtc
    15181 gcataatgaa cgtccgcaat aactcatcca cagcgccagg caaaccatca cattgcacat
    15241 aatgccgagg ctgactgatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa
    15301 aaccgcgact ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa
    15361 actgaaacag gcaatacctg ccagattacc gcaggccacc agaagtgcat taatcagcca
    15421 tgttcgccag ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa
    15481 caggctggtg ccgcaaaccg ctagcaaaat aaagccgagt gtaaagcaaa ggccgcccac
    15541 cagatgggtt aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa
    15601 tccaatcgcc ataaacacgc cagcggacat cgccattaaa aagaccgata ctttgctgcg
    15661 ggcaatttta tgggcagcaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa
    15721 gtcgaaagag agtttgtttc tcatgcatac tcctgcaaaa acagattaca acacctcgcg
    15781 aaccgagatc cccagccgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg
    15841 cgtcgccgcg ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat
    15901 gcgctgacgt tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gtgccattga
    15961 actgcgaagc gctgaatctt catttagcgt cggcagtaaa cggctttgtc ggacattaag
    16021 atgtaaattc agactgttac cgcgagtcag tagtaccgct cgctcaatca cgttttccag
    16081 ctcgcgcacg ttgcccggcc aatcccacga catcaactgg cgtagtgcct cggtcgggat
    16141 ggcgtcaatt gcgcgattca tatggcgcgc cattttttgc gtgaaatgtt ttgctaaaag
    16201 agggatatct tccggacggt cgcgcagcgg cggcaattcc agtgggaaga cattcaggcg
    16261 ataaaagaga tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt
    16321 ggcggcaatg actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc
    16381 ccgttcctgc aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc
    16441 atcgagaaac aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc
    16501 accagtaaat gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat
    16561 ggcagcgcag ttgattttga ccagtggctt gtcgcgtcgt gggctaagtt gatggatcgc
    16621 tctggcgatc acctctttgc cggttccggt ttcaccacaa atcaacaccg tactgacgct
    16681 cttcgccaca atatctacct gctggagcaa atcttccatt gcctggcttt gatagataat
    16741 gtcaccgacg cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa
    16801 gctttcctgc aaatcggtca tgctgcgcca ggcatcggca ttgccaacgg caatagcgat
    16861 gcgatcggca atatgttgta gtagctggca gttttcctca ctaaagagag tggaagaggt
    16921 atgcgccagc aacaatgcgc ccggtgtatg gttgccaaag gtgagcggta tcaggagcgc
    16981 agattcgcag ccgttagatg caagtaagtg taataacggg tcgcgttgcc agagaaacag
    17041 atcgtctgca cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac
    17101 actttcgcca ggcatacagc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca
    17161 ccacaggctg aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag
    17221 accgaaaaaa tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg
    17281 tgacaacact gcattagtga tatccaccag tacccgatga tgatcgcgat ctttctccag
    17341 caaacgcagc gccacctgct gttcttcatc attgagccat gattgcagaa ccggagtgat
    17401 tatgtgtgcc agccagttaa acagtgtgca atcctgggga gtaaaacggc tgccgtgagt
    17461 gcgaataaag cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata
    17521 atcgaccatc tcaccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta
    17581 gcggtaatag aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg
    17641 cggcggcagc gggatgaagg cgcgtaataa agacgccttg ccgtgtttct gcgctaaccg
    17701 ctccgcgagc attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc
    17761 ctcgtctgac atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt
    17821 ctggccatca gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga
    17881 atgctacgcg cctgccacgc cgacgtcgcg tcatctgcca gtggcgcgaa tgcccgggtc
    17941 aacagggcgg tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc
    18001 ttacgtcggg cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg
    18061 cagcggaagg cgacattcag acaatcaatc acgccaacgt gatggccgat agccaacgag
    18121 taatacatta cctgggagct gtgttccggc atctcgtcgc tactggcgat aaattttttt
    18181 cgcagcatcc agaaaacaat ttccctgcac tcctcagcca tgtaaccccc ggagaacgcg
    18241 aaagagttgc tgaacgatac tattgagacg cggatcgtcg gcttcgcgca accaggtgtt
    18301 aacccgattt cctgtgggat cgtcgcttaa atggcgcagg agtcgatcgc aaatttctcg
    18361 cccttgacga tagcccgcca gccgcctcgc ttctcgctca atggcgatac gctgtgatgg
    18421 cgggatctgc ggccacagcg gttgcatggt caccccggtg ggatcgcgat aatccaccgc
    18481 gtgaatcttc tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg
    18541 tgttggcggg cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca
    18601 gacgctgtag agatcgtgga aaataccgcc gccgacacca cacgcgccgt aggaaacaca
    18661 gattttatga tcgggggcag actcatatgc ccgaagtgca ggcatacgca tcgcccgggt
    18721 gactgcgcca gtaaataaca aaatatcggc gtgacgcggt gatgaaacaa ccttaatgcc
    18781 aaaacgttct gcgtcgaata cgggtgtgat ggcggcaaaa atttcgattt cgcaggcgtt
    18841 acagccgccg caatcgacgc gataaacgta ggccgagcga cggatatcct gtagcaaatg
    18901 ctgcttcatc ttttgcgttt gctcgtccag cgttatgggc tggctgacat gttgcgtaag
    18961 cactggactc atagctgctc cttagccacc agcggttcat ctgtgtcgtc gttgatcagc
    19021 gtcgcgcgtt gtttgcattc cgggcagacg ctcgcttgcg cccgcaacat ttcgcggttt
    19081 tgtggtgcat tttgttgctg tgccaacagt tctgcagcca gtgcgacggt tttttgcggg
    19141 gcaaacgggc gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga
    19201 tcggctttat tggtgacggt cagttcaaag ttattggtaa gctgaatggc tctggtcggg
    19261 cacacttctt cacaacgtcc gcagtaaata caacgtccca gatagagctg ccaggtgcgc
    19321 gtattttgct ggtagtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgcag
    19381 gcaccgcagg caatacattg gctgggcatc aggtccggtt tcccgcgaaa gcctgggctg
    19441 acctccagtg gtgcgaaggg atatttcacc gtcgcggttc cggcgcgcat aatagttttc
    19501 agtaacttca gcatcttctg tccttacttc agcggcgaac ggttacgatc aatgccgtag
    19561 cgttcgatct ctttatacgg cacgattttt gactggcgct tgcgcacatc aaccagcgtc
    19621 acacggtcgg tacaggagta gcagggatca aggctaccga taattagcgg tgcgtcagaa
    19681 acggtattgc cgcgcagcat gtaacgcagc actggccagt tggcgtaggt ggcggcacga
    19741 cagcgccagc ggaacaattt ttggttatca ccgagcatgc tccagtgcac gtcttcacca
    19801 cgtggcgctt caacaaagcc cagcgcgaat gcgtgaggtt tatagctaaa gccttcggtc
    19861 agcagtgggg tatctggcat gttgtcgagg gcaaattcca gcattgccag cgaatcaaac
    19921 gtctctttga cacggaccat cacgcgggag aagacatcgc cgccggtaaa ggtaaacagc
    19981 gtttttggaa tattgccgta gtcggcgtag gggtgatcaa agcgcaaatc acgggcaaaa
    20041 ccactgccgc ggatcagcgg cccgacaggg ctataatcac gggcgatttg tcggtcgaga
    20101 atgccaatgc cctgagtgcg ttgttccata ttcggcgtgg caagcagcat ctctaccagc
    20161 tccgacacgt cggcgcgcat ctcgcgcact agtttcagcg tttgcagacg ttgctctttg
    20221 agaatatcgc ggcgaacacc accaatcaga ttcagaccgt aggttttacg cgacccggtc
    20281 agcaattccg ccatcgtcat cgacttttcc cgcacgcgga aaaattgcat aaagccggta
    20341 tcaaaaccaa caaaatgaca ggagaggcca aggttgagca aatgactgtg cagccgttcg
    20401 acttccagca gaatcgagcg aatagtatgc gctcgttgcg gcacctcaat ccccagtgca
    20461 ttttcaaccg agttggtata ggccacactg tgggcaaaac cacaaatccc acacacgcgg
    20521 tcggataaga aggtcacttc gttgtagccc atccgcgttt ctgccagttt ctccatgccg
    20581 cgatggacat aaaacaggcg gtaatcagca tcgacaattt gctcgccatc cacaaacaag
    20641 cggaagtgac ctggttcatc ggaggtgata tgcagcgggc cgacagggat cacccgcgca
    20701 tcgctgttgc cctcgttgat aaacggatac gtttcggtat cagtcgtcgg ttctgggcgc
    20761 agtcgataat ccatcgcgtc tttgcgcagc ggatgcatat cttccggcca gtcatcgggc
    20821 aacaccagcc gacgctgatc cggcaggcca accggaatca gcccgtacat atcgcgaatt
    20881 tctcgctcgc cccagaccgc ggcagggacg cgcggtgtga ctgacggaaa ctcccgactg
    20941 tcggcatcga ccagtgcctt caccacgatc cagcattttt cggccccttc catagaaagg
    21001 gcgtaataaa ccgcgtaatg accgttaagt gtccgctcgt cgttgccaaa caggaccgga
    21061 agccagccat catgttgata ataaagataa tgtacaacgt caggcagcag attgattttc
    21121 accgtaatgg tgacctgttc aggcgtttgt cgctcttcat ccagcaccgc gccggggaac
    21181 tgcgttttca gggcggcgag aatcgcttcg ccacgatttg acgatgaatt aacgttcact
    21241 gtgtttctcc gaaacagatg cggtgcctga aggcaaaaaa tcatgccagg tgctacgttg
    21301 tgcaggcagg ttgtgcgtcc ctgagaggac tatagtggaa gcgcccgcca ggatcctgat
    21361 gacaggttgt ggaatatgcg ttcccatcgc cagcatcatg accagcagaa tcaccattgg
    21421 cgaggtggtc aaccagccaa gttcaccccg gttaacggcc tgcggcggtt tcgccattaa
    21481 tacccgtgca gccatccgta ccaggcccgc cagcaccagc gttaacagca ataacagcag
    21541 gacgataagc agcagatgat tacgcgccag tccggcggta acggtcataa attcgctaag
    21601 aaaaatgttg aagggcggca tcccggccag cgccagcgca ccgccgccaa acaacacggc
    21661 ggtaaatggc atgattttga gcatcccaca gacgacgttg agatcgcgcg tgccgtactt
    21721 gagcagtaca ttgccggaac cgcagaacaa cagcgttttg gccagactgt ggtttaaggt
    21781 gtgcagcagc gcggcaaaaa ttcccagcgg cccgccaatg cctaacgcca ccgcgaccag
    21841 tcccatgttc tccacgctgg agtacgccag cagacgctta atgtcccgct gtaccagaat
    21901 gaaaaatgcc gccacggcaa ccgacaacat gccgaagatg agcaacaacc ggttggggaa
    21961 atcgctgccg atggcctggc aaataatgat gtaatagcga ataagcacca acagcgcgca
    22021 gttcagcaat acggcggaga gcagggcgct gaccggactc ggcgcttcac tgtgagcatc
    22081 tggcagccag gcgtgcatgg ggaatagccc ggttttggtg ccaaagccaa ttagcacaaa
    22141 cacaaaggcc aacagcatta atgttgggtc aagcaaggac gattgcttaa gaacctcgct
    22201 ccagaatatc gccatttctg cctgcggcat aacgctggcg gcgttggcgt ataccagcac
    22261 ggtaccgaac agaccaaaag cgacaccaac agtacaaata atgatgtact tccatgcagc
    22321 ttccagcgat gaacgctgac cgtaaatgcc taccagaaac gccgagctta aggtggtggc
    22381 ttcaattgcc gcccacatca caatcaggtt attgctggta acaaccagca gcatggtgaa
    22441 caaaaacaga tggaagaaac cgtagtaatc gcacagcgta acgggtgaaa gctcgccgtg
    22501 tgccacttca tgacgcatgt agccaatcga gtaaacaccg gtgagaaagc caatcacacc
    22561 aagaatggcg aggaacaaac cgcccagacc atcaatgtga agccacagtc ccgcagcgaa
    22621 tatttctccc gcatcagcgg cagtttggac cacccacagt gccagaatca gcagcagtgt
    22681 gatccctaag ccatgtagta ctgtcaccgt gcgagtcgca gagtgccccc gtttccggca
    22741 ggcaaaacag agcagcgaaa aaagcagcgg cgtgagcagg agtaaagcga acatcacaga
    22801 ataactcatc tcattatccc ttcagcgcgg tcaagttatt cacgtccagc gtgccgtggg
    22861 tacgccatat ttttcttgcc agtaacacca tcacaatgac ggcgaagatg gcgtcggtag
    22921 cgatacctat ttccaccagt tccggtgctc gccaggcaag aagcgccagc accagatggg
    22981 agccgttttc catcaggcag taaccaaaaa tttgccgcag gatattgcgc tggctgacaa
    23041 tgcacagcag gccaagcaga aaatgaccta acgccaccgc cagcgccggt ttcagcccgg
    23101 tagccatcgg tagcttcacg ggctgaacga caaatgcgca aagcaggaca attaacgccg
    23161 cgagcagtgt catcattgcc ggaccgaata acgctttttc cgggatgttc tggggaatat
    23221 ttcgtgcagc gtaagtcatg attaacggta ccagcagcac tttggtgata aaggcgctgg
    23281 cggaccagat cagcagttgc tctgcgacga acaggcacga gagggtggcg aaaatagaca
    23341 ccagcaccag cgactggcag gcgtaaaatc cgcatgacag gcgatagctt ttgacgctaa
    23401 tcacaaacag cgatgtcagc atcatcagtc ccgccagatt atttacgatc atagaaccgg
    23461 tcattcatca tctccttact gcaaccaggt cgcggcgatt acgctggaaa tcagcgacat
    23521 cacaatcagt acaatcaaca ccaggcgcat tgatcctggc agcggtgcgg catcggcgac
    23581 ggcctcgctc ggtttgccag gcacgacgcg accaaaccag cgaataaacc aggcgaaact
    23641 ggcgaccgat tcaatcatca gcagaatcat ggcgggcagc aggatccagt actccactga
    23701 caacgcaaaa ccggcggcaa acagcgggaa tttactgaag aagccgttga acggcggtac
    23761 gccggtaatc gccagcgctg ccacgcagaa acccacgcct ggcaacggca gggtgtgcaa
    23821 tacgccacgc agacgcggca acaagcgcgt gccgcagctg taactcagcg cacctgctac
    23881 aaggaaaaat aggcttttag cgaacgcgtg gttgacgatg taggcgatgc tgccctccag
    23941 cgccagccgc gagccgaaga tggagagcga caagccgaaa aacatccagc caagttgagt
    24001 gatggtcgac caggccagca accgcttcat atcctgctgt ggcaaataca tcaggaagcc
    24061 ataaagaatg gtgaccagtg ccataaccat gccaacgccg ccaatcacat gcgggatatt
    24121 gccgccgtcg ataatggcgc gggcaaaaat gtaaacgccc actttcacca tcgatgcggc
    24181 gtggagatag gcgctgatcg gtgttggcgc ttccattgca tccggtagcc acgcttgcat
    24241 cggtagctgg gccgatttcc cccacgcggc aaacaggatg ccaccataaa ccagatagcg
    24301 tgcgtcgccg tgtaactcgc tcatcgcgct gagcgcgaac gttccggtct gcaaaaacag
    24361 cgtggcggcg gcaagataca accccaacga accgatatga gtgataagca acgcttttag
    24421 tgctgaacgc tgcgctttat cgctctggta ataactgatc aacgcccagg agcagccgcc
    24481 cgtaatttca aaaaacaaca actgaccgag cagcgtcgag gagagtacca gtcccgccat
    24541 cgcgccgata aaaaccagca aaaatgcgta ataacgattc gtgccgttat gcgggtgttc
    24601 gcgattttta tccgtcagat aacccgtgga gtagatcgtg accagcaaac cgagaaacac
    24661 caccacaaac agaatcagcg tactcacgcg atcaatgacc aggccaaaca acgccacacc
    24721 ccccacgttg accaacgtca acgtgacggc aactttatcg gcctgataaa acgcggagat
    24781 cagtgacaac atgcacagcg tggtcagcgc ggcgaacaaa accccccatt cggcggcccg
    24841 acgttgtggc gaaaacgaaa cgaccagtgc gccgataaaa ggcagcaata acgtcgtcag
    24901 agcaagattt tccatattcc gtcagtgctc cttacagacc ggttaaccag aagacccatg
    24961 caagcgcagc aaggctgaag ccaagccagg tcacatggtg aatgagtaaa aatcgcccgc
    25021 gtgccagcgt gttttctgcg attgaggcca gtacaaaaat cagcaaaacc ttgagcagcg
    25081 taacgacaag tgaagtcagc aggcaggcga gagaaagctc ttgcgcgcgc ccaaagggca
    25141 gaaacagggc cacaaacagt gatgccatca cgacctgttt cagccccagc cccaacttcg
    25201 ccagcgccag cccggcaccg gaatattcgg tcagcgggcc ttcctgtaat tcctgttctg
    25261 cttcagcaac atcaaaggga atttttccca tctcaatgaa gcaggcaaaa ccacaggcca
    25321 gtaacgccag tacggtggtt agcggcgagt tccagcccgt cgccagcgtc ttgctgatca
    25381 tctcgatatg cgtggaatcc gctatcagcg ccaataccag cagtgagaga atgagcatgg
    25441 gttcgaccag aatgccgagc gtcaactcgc gactggcacc gactcccgca aacgggcttc
    25501 cggtatccag cccggaaaga gcaaagaaaa aatgaaacag ggcaagaaga tagataaggg
    25561 tgatcagatc gccgccgccc gcaaaagggg aaacagtaat aaatagtggc agggccatcg
    25621 ccagcaccag catgctgctg attaataccc acggcatcag gcggaacatc agacctgaag
    25681 atgtcggcgc aacttcctgg cgtttaaaca gtttgtggat atcgcgataa tcctgccaga
    25741 accccggtcc gcgacgggag tgcattcgcg cgcgtatctg ccgggaaatc cccgtaaaaa
    25801 gcggggttag caccagcaga gtcacggcct gtgctaacgc aaaaatgacc agatatccgt
    25861 cgcaaagagt ttgtctcatg gtgatttcct tagacggcaa tagcgattag cagcacaacc
    25921 agtgcggcga ccacatacag gcaatagaga cgaaagtcgc cactttgcag atgctgtatt
    25981 tctttggcca gccgttgggt ggcgctcacg atggggcgga tcacccgctc atcccagaag
    26041 ggttctgtgc tctgagccct ggcggtgacg tgcgcaagac ctttgttcag cctcagcgta
    26101 gggtcgagct gttgtcgtag acgaaatagc gcacaaaaga ccacacgcag cggctgcatc
    26161 acgccattgc ctgacggggc catcgcatgt tcccagccgt agccgcatgc ccaggcatct
    26221 ccagtgcgac gaaacgctgc gcggcgcgaa cgacaccaca gccagtaaag gccaggcagc
    26281 aaaggcatcg ccagtagtaa cagcaacagt aatgagggag tgacccgtgt atgaaacgtg
    26341 ccgggtacaa gtgctattcc gctggtgaca gtgacgggag gggtattggt aaacgcatgg
    26401 gcaatgtgca ttatcttcgg tgccagccaa ctggcactaa gcgcaatgaa tacgcagagt
    26461 gccgcgagca gtagcatggc gacgatcatc gtatttggca cttcctgtgc ctcttcagcg
    26521 tgtgtactgc gcggcgcgcc acagaaagta ataccgtaca ttttgacgaa gcacattact
    26581 gccagcccac cagtgactgc cagcatcacc atagcaatag gacccgcaag ttgtagcgct
    26641 acggcttcca cacgacttag tgagaacagc gactgccagg tgtaccattc gctgataaaa
    26701 ccattcagcg gaggaatggc tgatatcgcg aggcaaccaa tcaggcatgc tgcggctgtc
    26761 cacggcatcc gtttcgccag tgcccccatt ttttccatgt cgtgggtatg caaacgcgaa
    26821 ataatcgcac ccgcgccgag aaacagcagc cctttgaaca gcgcatggtt gagcagatga
    26881 aacagcgcgc cgagcagtcc aacaacggtg agcagcgggt cgtgcagtga cagaccgacc
    26941 atcgccacac cgaccgccag caaaataatg ccgacgtttt cgacggtgct ccaggccagc
    27001 agccgtttga tatcctgttc cgccagcgca tacagcacgc ccaggagcgc ggagattgcg
    27061 ccgatcgcca tcaccagaat gccccaccac agcggcaaac ccgtttgcgc cagcagatca
    27121 atcgctactt tcaggatgcc gaaaataccg attttgacca ttacgccaga catcaacgcc
    27181 gaagcgtgcg atggtgcggc agggtgagcg cgtggcaacc agctgtgcaa cggcatcatc
    27241 ccggctttcg cgccaaaacc gaaaaaggcc agcaggaaca ccgccgacgc cagccccgga
    27301 gaaagtgaaa gcgtgcggaa actggcaaaa tcgaggctgc cgctttcgcg ccccatcagc
    27361 aagaaggcga tcataatcag caccgatccg gcgtgggcga taaaaaagta gagcatgcca
    27421 gcgttgatcg acgttttatc ctgcctggca atgaccagaa accaggaaga cagcgacatc
    27481 atttcaaaca gcacgatgaa ccaaaaagcg ttgtccatca ccagcagggc aaccatcgat
    27541 gcgatgaaaa gattcataaa gaagctcatc gccgccgcgc ctttaccctc gtattcgcgc
    27601 atataagtca atgaatagag cgaacaaacc accaccagca aggagataac catcaccata
    27661 aatgccgaga ggctatccat tcgcagtgtt aaatcggcaa agtcaaaagg cgtggcaaaa
    27721 cgggcgacta aagttacgcc tgcatgtaat tgcgtaattc cgctgatgat gccaatcacg
    27781 ccacccacca gcgatgtgat gccggaaagc ttaatagcca gtctgtccag accgagtaaa
    27841 aacagcgaag ccagactggc aaacagatag aggatcagtg accaggttaa taattgcagg
    27901 gcatccatta gcgttgctcc tgagtgaggg aaaggggatc ctccccgcca ttggcaaacc
    27961 agcttgcggc aaggcgctgt ttttctttca tctgacgttg caggctatcg tcggtgatca
    28021 gccgtaacgc ctgattcggg caagcgcgaa cgcaggccgg accttctggc aagaaatcac
    28081 acaggtcgca tttcaccgcg acggtctgga cacccggttc ccagcgcagc aaagcatgtt
    28141 gggtcggcgc gttttcttcg ccgtctttga gtgagccgtc agcctgaaaa acatattgcg
    28201 caggggcatt caccggacgg cttcctgaag cgctgattgc gccaaatggg cagaccacgg
    28261 cgcaaagctt gcagccaata cagaggcttt cgttgagttg gatcgcatcg tccctctggg
    28321 agatggcatt gaccgggcag acctgcaggc aaggggcttc ctcacagtga tgacacacga
    28381 caggggcagt gagtgttgac gtcttcgcca gggccaggcg cgggtgttgc tgtaaacctt
    28441 gcgttttatg cacgtccgaa caggcagcga gacaggtatt acatcctgta caccacagtg
    28501 gttcggccac cacaaagcgg ttcatgtcgg ctccttgaaa atgatatgaa acattgcatt
    28561 tcatattgca taaaggatgc cagtctgatt aatttaatgt attcaataag ttatgaatta
    28621 actgtattct gaacggtaca aaaatgatgg tcattaacgt tcagcaaaaa atgaattcga
    28681 cacccgtgtc gcaggggaaa gcgagaatgt gatcttgatc acgagaagtg aggtgtgctg
    28741 accgcaagcg cagccagcac ggaatggagc aaagtaatca ggcgtgttct ttcagccagt
    28801 tcagcacaac gtcgtggtga ttgctggttt tgaaatcgtc aaagacatgt tcgattttgc
    28861 catcagcgtc aatcaggaag ctgatgcgat gaataccatc gtaggttttg cccatgaagg
    28921 acttttcacc ccagacgccg aattgttcgc acacctggtg gtcctcatca gacaggagcg
    28981 taaagttaag cagctctttt tccgcaaaac gggagagttt ttcgggttta tcggtgctga
    29041 tacccagcac atcaacgccc gcttttttca actcatccat gttatcgcgt aagccgcagg
    29101 cctgtacggt acagccgggg gtcatggctt tcgggtagaa ataaaccaga acacgctgtc
    29161 cctggaagtc ggtcaaatta acttgttctc cgtcttgatc cggcaagcta aatttcggtg
    29221 cgatatcacc ggctttcagt ggattcatta cttaactcca tcctgttcat catgttggga
    29281 ataattgacg acgttaatac tgccttgtgc attgagttct gtacataggg ctttgaacgc
    29341 ttgctcaata tttgctgcgt ccgcagatgc ggggctgtgg gcggttatct gaatatgcaa
    29401 ctgcgcagcc cgttcatttt cagcaggttg cgtgcgcgac accagctccg caatgttcat
    29461 atgatgcgcg tcgaaaagtg ctgtgaagcg ttcaattaaa tgcggggagt ctgccacatc
    29521 gacctgaacc cagacagatg ctggcattgg cggacgcgga cgcgccgtcg tgcgcttcat
    29581 cacgattaaa agatccagtt ctgcaccttt caacggtaac gttgattcaa tcagagtaat
    29641 ggcattccat gaaccggaaa gcagcatgat aaacgtgaac tcttctccca gcatcgccag
    29701 gcgactgtct tcaatattac agccgcaact actgacatga cgggtgatgg tgttcacaat
    29761 tccagggcga tcggcaccca acgcagtgat caccagataa tgttgcgatg acagtgtcaa
    29821 actggttatt cctttaaggg gtgagttgtt cttaaggaaa gcataaaaaa aacatgcata
    29881 caacaatcag aacggttctg tctgcttgct tttaatgcca taccaaacgt accattgaga
    29941 cacttgtttg cacagaggat ggcccatgtt cacgggaagt attgtcgcga ttgttactcc
    30001 gatggatgaa aaaggtaatg tctgtcgggc tagcttgaaa aaactgattg attatcatgt
    30061 cgccagcggt acttcggcga tcgtttctgt tggcaccact ggcgagtccg ctaccttaaa
    30121 tcatgacgaa catgctgatg tggtgatgat gacgctggag ctggctgacg ggcgcattcc
    30181 ggtgattgcc gggaccggcg ctaacgctac tgcggaagcc attagcctga cgcagcgctt
    30241 caatgacagt ggtatcgtcg gctgcctgac ggtaacccct tactacaatc gtccgtcgca
    30301 agaaggtttg tatcagcatt tcaaagccat cgctgaacat actgacctgc cgcaaattct
    30361 gtataatgtg ccgtcccgta ctggctgcga tctgcttccg gaaacggtgg gccgtctggc
    30421 gaaagtaaaa aatattatcg gaatcaaaga ggcaacaggg aacttaacgc gtgtaaacca
    30481 gatcaaagag ctggtttcag atgattttgt tctgctgagc ggcgatgatg cgagcgcgct
    30541 ggacttcatg caattaggcg gtcatggggt tatttccgtt acggctaacg tcgcagcgcg
    30601 tgatatggcc cagatgtgca aactggcagc agaagggcat tttgccgagg cacgcgttat
    30661 taatcagcgt ctgatgccat tacacaacaa actatttgtc gaacccaatc caatcccggt
    30721 gaaatgggca tgtaaggaac tgggtcttgt ggcgaccgat acgctgcgcc tgccaatgac
    30781 acctatcacc gacagtggtc gtgagacggt cagagcggcg cttaagcatg ccggtttgct
    30841 gtaaagttta gggagatttg atggcttact ctgttcaaaa gtcgcgcctg gcaaaggttg
    30901 cgggtgtttc gcttgtttta ttactcgctg cctgtagttc tgactcacgc tataagcgtc
    30961 aggtcagtgg tgatgaagcc tacctggaag cggcaccgct tgcggagctt catgccccgg
    31021 ctggaatgat tttgccggtg acctccggtg attatgcaat cccggtgacc aacggtagtg
    31081 gtgctgtcgg taaggcgctg gacattcgtc caccagccca gccgctggca ctggtttctg
    31141 gcgcgcgtac ccagttcacg ggcgataccg cttcattgct ggtggaaaat ggtcgtggca
    31201 atactctgtg gccgcaggtg gttagcgtgc tgcaggcgaa aaactacacc atcacccaac
    31261 gtgatgatgc tggtcagaca ctgaccaccg attgggtaca atggaaccgt ctggacgaag
    31321 acgagcagta tcgtggtcgt tatcaaatct ctgttaagcc gcagggttat cagcaggcgg
    31381 ttacggttaa actgctgaac ctggaacagg cgggcaaacc ggttgcagac gcggcttcca
    31441 tgcagcgtta cagcacggag atgatgaacg ttatttccgc cggtctggat aaatctgcca
    31501 ctgacgccgc gaacgctgcg caaaatcgtg cctccaccac tatggacgta caaagtgcag
    31561 ctgatgacac cggtttaccg atgctggtcg tacgcgggcc gttcaatgtg gtttggcaac
    31621 gtctgccagc ggcgctggaa aaagtgggca tgaaagtgac cgacagcacc cgttcgcagg
    31681 gtaacatggc cgtaacttac aagccgctgt ctgacagcga ctggcaggaa ctgggcgcga
    31741 gcgatccagg cctggcatcc ggtgactata aactgcaggt tggcgattta gataaccgca
    31801 gcagcctaca gttcatcgat ccgaaaggtc atactctgac tcagagtcag aacgacgcgc
    31861 tggtagctgt cttccaggct gcgtttagca agtaaaaata cagggctgga gtcatccggc
    31921 ccttttttct gatatgatac gcaaacgtgt gcgtcggcag gaaaacgcga ttttagcggt
    31981 aattcgcact aaatttgttt gtcggacgta ggtcggataa ggcgttcttg ccgcatctga
    32041 caaaacatcc ggcacaccag acagcaaaag attttaaaac gttaattcac acccaggagt
    32101 gataaagatg caaaagcaag ctgagttgta tcgtggtaaa gcgaaaaccg tatacagcac
    32161 ggaaaacccg gacctgttgg tgctcgaatt ccgcaatgat acgtcagcag gggatggcgc
    32221 gcgcattgag cagtttgatc gcaaaggtat ggtgaacaac aagttcaact acttcattat
    32281 gagcaaactg gctgaagcgg gtatcccgac tcaaatggag cgtctgctct ccgataccga
    32341 atgtctggtg aaaaagctgg atatggtgcc ggttgagtgt gtcgtgcgta accgtgctgc
    32401 tggctctctg gtgaaacgtc ttggaatcga agaaggtatt gagctgaacc cgccgctgtt
    32461 cgatctgttc ctgaaaaacg acgccatgca cgatccgatg gtcaacgaat cttactgcga
    32521 aacctttggc tgggtgagca aagagaacct ggcgcgtatg aaagagctga cctacaaagc
    32581 gaacgacgtg ctgaaaaaac tgttcgatga tgctggtctg attttggtcg actttaaact
    32641 ggagtttggc ctgtacaaag gtgaagtggt actgggcgat gagttctccc cggacggcag
    32701 ccgcctgtgg gacaaagaaa cgctggagaa aatggacaaa gaccgtttcc gccagagcct
    32761 cggtggcctg atcgaagcct atgaagccgt cgcccgccgc ctgggtgtac agctggactg
    32821 atttttctgt tcatcatctt gccgtgcagc tggcacggca agacaaccgc tctcggaaga
    32881 tgtgcattga acgctcattc ctccgccatt tcatcccgct ttcctgtggt aatcttcctc
    32941 tgaaccacct acgatcattt ctataatgaa tatattgttg aggtatctat gcgttggcaa
    33001 gggcgacgtg aaagtgacaa tgttgaagac aggcgcaaca gctctggtgg tccgtcaatg
    33061 ggcggtcccg gttttcgcct gccaagcggt aaaggcgggc tgattttact gatagtcgtg
    33121 ctggttgcag gctactatgg cgttgattta accgggttga tgaccggaca gccggtttcc
    33181 caacagcaat caacacggtc aattagtcct aatgaagacg aagccgcaaa attcacttcg
    33241 gtgattctgg caaccacgga agacacctgg ggacagcaat tcgagaagat gggtaaaacc
    33301 tatcagcaac cgaagctggt catgtaccgt ggaatgacgc gcaccggctg cggggcgggc
    33361 cagtccatta tgggaccgtt ctattgcccg gcggatggca cggtttatat cgatctctct
    33421 ttctatgatg acatgaaaga caaacttggc gcggatggcg attttgccca ggggtacgtt
    33481 atcgcccatg aagtcggtca tcatgtgcag aaactgttag gcatcgagcc gaaagttcgt
    33541 caactgcaac aaaacgcgac gcaggcggaa gtgaaccgct tatctgtgcg tatggaactc
    33601 caggccgact gttttgccgg tgtctggggg catagtatgc agcagcaagg tgttctggaa
    33661 accggcgatc tggaagaggc gctgaacgcg gcgcaggcca tcggcgatga ccgtttacaa
    33721 cagcaaagtc aggggcgagt agtaccagac agtttcactc atggcacttc tcagcaacgc
    33781 tacagctggt ttaaacgtgg tttcgacagc ggtgatccgg cacaatgcaa tacttttggt
    33841 aaaagcattt aacttccggg gcagggatgg ctgaactgac tgcgcttcac acattaacag
    33901 cgcaaatgaa acgtgaaggg atccgccgct tgctggtgtt gagcggggaa gagggttggt
    33961 gttttgatca tgcgcttaag ttgcgtgatg ccttacctgg cgactggctg tggatttcgc
    34021 cgcagccagt cgctgaaaac cactgttttc cctcggcgct acaaacttta cttgggcgcg
    34081 agttccggca tgcggtattc gacgcccgcc acggctttga tgccgctgcc tttgcggcac
    34141 ttagcggaac gttgaaagcg ggaagttggc tggttttgtt actccctgta tgggaagagt
    34201 gggaaaacca acctgatgcc gactcgctgc gctggagtga ttgccctgac cctattgcga
    34261 cgccgcattt tgtccagcat ctcaaacgcg tacttacggc gaataacgac gctatcctct
    34321 ggcggcagaa ccagcagttc tcgttggcgc attttgctcc ccgtactgac tggcaccccg
    34381 ctaccggcgc accacaacca gaacaacagc aactcttaca gcagttactg accatgccgc
    34441 cgggcgtggc agcggtaact gctgcgcgtg ggcgcggtaa atcggcgttg gcagggcaac
    34501 tcatttctcg tattgcgggc agtgcgattg tcaccgcgcc cgcaaaagcg gcaacggatg
    34561 tactggcaca atttgcgggc gagaagtttc gctttattgc accggatgcc ttgttagcca
    34621 gcgatgagca agccgactgg ctggtggtcg atgaagccgc agccatacct gcgccgttgt
    34681 tgcatcaact ggtatcgcgt tttcctcgaa cgttgttaac cactacggtg cagggctacg
    34741 aaggcaccgg acgtggtttt ttgctgaaat tttgcgctcg ctttccgcat ttacaccgtt
    34801 ttgaactgca acaaccgatc cgctgggcgc agggatgccc gctggaaaaa atggttagtg
    34861 aggcactggt gtttgacgat gaaaacttca cccatacacc acaaggcaat atcgtcattg
    34921 ccgcatttga acagacgtta tggcgaagtg agccagaaac gccgttaaag gtttatcaat
    34981 tattgtctgg tgcgcactac cggacttcgc cgctggattt acgccgcatg atggatgcac
    35041 caggtcaaca ttttttacag gcggctggcg aaaacgagat tgccggagcg ctgtggctgg
    35101 tggatgaggg cggattatct caagaactca gtcaggcggt atgggcgggt tttcgtcgcc
    35161 cgcggggtaa tctggtggcc cagtcgctgg cggcgcacgg cagcaatcca ctggcggcga
    35221 cattgcgcgg acggcgggtt agccggatag cagttcatcc tgctcgtcag cgggaaggca
    35281 cagggcgaca gcttattgtt ggtgctttgc aatatacgca tgacctcgac tatctttcgg
    35341 tgagttttgg ttacaccgag gagttatggc gtttctggca acgctgcggt tttgtgctgg
    35401 tgcgaatggg taatcatcgt gaagccagca gcggttgcta tacggcgatg gcgctgttac
    35461 cgatgagtga tgcgggtaaa cagctggctg aacgtgagca ttaccgttta cgtcgcgatg
    35521 cgcaagctct cgcgcagtgg aatggcgaaa cgcttcctgt tgatccacta aacgatgccg
    35581 tcctttctga cgacgactgg cttgaactgg ccggttttgc tttcactcat cgtccgctat
    35641 taacgtcgtt aggttgctta ttgcgtctgc tacaaaccag cgaactggca ttaccggcgc
    35701 tgcgtgggcg tttacagaaa aacgccagtg acgcgcagtt atgtaccaca cttaaacttt
    35761 caggccgtaa gctgttactg gtccgtcaac gcgaagaagc cgcgcaggcg ctgttcgcac
    35821 ttaatgatgt tcgcactgag cgtctgcgcg atcgcataac gcaatggcaa ttttttcact
    35881 gactccttca attttctccc atggtcattg tgagtcagcg gcgtaaaatt aatgacatca
    35941 gttaaggagt tcgccatgaa acatgaccat tttgttgttc aaagcccgga taaacctgcg
    36001 caacagttgt tactgctttt ccacggtgtc ggggataacc cggtggcaat gggggaaata
    36061 ggcagctggt ttgcgccgct atttcctgat gcgctggtgg tgagtgtcgg aggcgcggag
    36121 ccgagcggta atccggcggg gcgtcagtgg ttttcggtgc agggtattac ggaagataat
    36181 cgccaggcac gtgtggatgc catcatgccg acgtttattg aaacggtgcg ctactggcag
    36241 aaacagagcg gagtaggggc taacgccacg gcactaatcg gtttctctca aggcgcgatt
    36301 atggcgctgg agagcattaa agccgaaccg ggccttgctt cgcgcgtcat tgcctttaac
    36361 ggacgctatg ccagcctgcc ggaaaccgcg tctacagcca cgacaattca cttgattcat
    36421 ggtggtgaag atccggttat cgatctggcg catgcggttg cggcgcagga agcgttaatc
    36481 agtgccggtg gtgacgtgac gctggatatc gtggaggatt taggccatgc gatcgacaat
    36541 cgcagtatgc aatttgccct ggatcacttg cgttatacca ttccgaaaca ttatttcgac
    36601 gaagcgctaa gtggcggtaa gcctggcaat gatgatgtga ttgaaatgat gtaataataa
    36661 ccgggtaggc ctgataagac tgtatcaggc atcatggccg gatgtggcgt gaacgcctta
    36721 tccggccgct cagcttactt cttcggttga tcctttttcg gccagtcgtc gtcatcgtcc
    36781 cacttatcgt tgaaatcacg atgcggggga agctccggct tgttggcgag aaatttctta
    36841 tggtcgacgc gcttgagatc tttaatcacg ttcagcagga cgcctaccaa aaagacaatc
    36901 accagaatcc accaatattt agccagccag tccatgctta tttcctcttg cagaaccact
    36961 catcaggcga cgagctgttc catgatacgt tgatacatac gggcaagtag ctgcaggtcg
    37021 gcagcgttca cacattcatt aattttatga atagtggcat tgaccggccc gagttccacc
    37081 acctgcgccc ccatgcgggc aataaagcgc ccgtcggacg ttccgcctgt ggtcagtagc
    37141 tgcggtttaa tttcattata gtgctcaacc gcgttaacga ccgcatccac cagtttaccg
    37201 cgcgcggtca aaaatggctg cccggaaagc caccattcca ccgtataacg cagttgatgt
    37261 ttttcaagca gggcaagtac ctgcgctttg atcatctcat cagtcagttc ggtgctgaag
    37321 cggaagttaa actgcacaaa cagttcaccc ggaataacgt tgttactgcc cgttcccgcc
    37381 tgaatattgg caatctgcat actggtcgcc gggaagaatt cattgccctg atcccactca
    37441 atagccacta attcattaag gaaaggtgct gcgcgatgta ccggattgtc agccagatgt
    37501 gggtaggcaa catgcccctg cacgccatga atggtgaggt tgcaggttaa cgatccgcga
    37561 cgaccatttt tcaccacatc acctaccact tcgatactcg acggttcgcc aaccaggcag
    37621 taatcgagac gctcattacg tgccatcaac gcttcgacga cttttaccgt accgttatgg
    37681 gcactggctt cctcatcaga agtgatcaga aatgccagtc gacctgcatg gttgggatgt
    37741 tgtgcgacaa agcgttctgc cgctaccacc atcgctgcca gcgagccttt catatctgcc
    37801 gcaccgcgcc cgaataacat gccatcacga atggtgggtt caaacggcgg attgatccaa
    37861 cgatcggcgt cgccaggcgg caccacgtcg gtatgcccgg caaaggccaa cgtttcacct
    37921 tgcccacgcc atgcccaaaa attctgcgta tcggcaaaat ccatgcgttc aacggtaaaa
    37981 ccgatcgcct gcaaacgttc aatcaacaaa gcctgacatc ctgcgtcatc aggactcagg
    38041 gaagggcggc gaataagctg ttgtgtcagc tcaataaccg ggcacgacat agactacacc
    38101 tcatggaaaa attgctgata actggaatca ctgaaaccca gcagcatagg cttaccgggc
    38161 gtgcagagca atggacgttt gataattgca ggcatttcag tcattaatgc cgccgcagag
    38221 gccgcatcga tgattttatt gcgggtggtt tcgtccagtt tacgccaggt tgtaccacgg
    38281 gtgttgagta acgcttccca gcctaattcg ttgataaaac cgttcaataa ttcgctgtcc
    38341 agcccatcga cgcggtaatc atgaaaacga tagtcgatgt tattggcttc cagccaacgg
    38401 cgagcctttt taatggtgtc acaatttttg atgccgtaaa gtgtaaccat aatgaatcct
    38461 ttttcgattt atgagaataa atactcattt aagag
//
LOCUS       DAOTRK010000026        38435 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.026, whole genome shotgun sequence.
ACCESSION   DAOTRK010000026 DAOTRK010000000
VERSION     DAOTRK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.026"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            142..684
                     /gene="ecpR"
                     /locus_tag="QIE41_001352"
                     /gene_synonym="matA"
     CDS             142..684
                     /gene="ecpR"
                     /locus_tag="QIE41_001352"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDS3530260.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVP"
     gene            759..1346
                     /gene="ecpA"
                     /locus_tag="QIE41_001353"
                     /gene_synonym="matB"
     CDS             759..1346
                     /gene="ecpA"
                     /locus_tag="QIE41_001353"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDS3530261.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVAIWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1403..2071
                     /gene="ecpB"
                     /locus_tag="QIE41_001354"
                     /gene_synonym="yagY"
     CDS             1403..2071
                     /gene="ecpB"
                     /locus_tag="QIE41_001354"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDS3530262.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2097..4622
                     /gene="ecpC"
                     /locus_tag="QIE41_001355"
                     /gene_synonym="yagX"
     CDS             2097..4622
                     /gene="ecpC"
                     /locus_tag="QIE41_001355"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDS3530263.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQNDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANALFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4612..6255
                     /gene="ecpD"
                     /locus_tag="QIE41_001356"
     CDS             4612..6255
                     /gene="ecpD"
                     /locus_tag="QIE41_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDS3530264.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6224..6934
                     /gene="ecpE"
                     /locus_tag="QIE41_001357"
     CDS             6224..6934
                     /gene="ecpE"
                     /locus_tag="QIE41_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDS3530265.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRITISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7247..7576
                     /locus_tag="QIE41_001358"
     CDS             7247..7576
                     /locus_tag="QIE41_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDS3530266.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7825..8439)
                     /locus_tag="QIE41_001359"
     CDS             complement(7825..8439)
                     /locus_tag="QIE41_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDS3530267.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8846..9535
                     /gene="paoA"
                     /locus_tag="QIE41_001360"
     CDS             8846..9535
                     /gene="paoA"
                     /locus_tag="QIE41_001360"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDS3530268.1"
                     /translation="MSNQGEYPENNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLDVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGDEITTIEGLGSPENLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDSIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9532..10488
                     /gene="paoB"
                     /locus_tag="QIE41_001361"
     CDS             9532..10488
                     /gene="paoB"
                     /locus_tag="QIE41_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDS3530269.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10485..12683
                     /gene="paoC"
                     /locus_tag="QIE41_001362"
     CDS             10485..12683
                     /gene="paoC"
                     /locus_tag="QIE41_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDS3530270.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWYEE
                     APNAAYGYIVGSAIAKGCLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPGQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12693..13646
                     /gene="paoD"
                     /locus_tag="QIE41_001363"
     CDS             12693..13646
                     /gene="paoD"
                     /locus_tag="QIE41_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDS3530271.1"
                     /translation="MSYSLFDKDEHWHKPEQAFLTDDHRAILRFAVEALMSSKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGHYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETEQ
                     IRAPVGIFPKARDAHTLALSVLAEVAAVRLRQEDSCLPPSS"
     gene            complement(13825..14952)
                     /locus_tag="QIE41_001364"
     CDS             complement(13825..14952)
                     /locus_tag="QIE41_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3530272.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLQLGMTVLMAVSGLIIALASSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPIA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSSILLIIGVAGFIGTLVVSAFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLAIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(15094..16152)
                     /locus_tag="QIE41_001365"
     CDS             complement(15094..16152)
                     /locus_tag="QIE41_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS3530273.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            16417..17301
                     /locus_tag="QIE41_001366"
     CDS             16417..17301
                     /locus_tag="QIE41_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3530274.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            complement(17735..18655)
                     /locus_tag="QIE41_001367"
     CDS             complement(17735..18655)
                     /locus_tag="QIE41_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDS3530275.1"
                     /translation="MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAVLKDKNPAKYVAAVNKLERVLA"
     gene            complement(18642..19424)
                     /locus_tag="QIE41_001368"
     CDS             complement(18642..19424)
                     /locus_tag="QIE41_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDS3530276.1"
                     /translation="MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRR
                     IAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLTSLALQGHAHYTEQGKPQVWLAL
                     PAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
                     CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYI
                     DQSGIETGTGKRQIIPGGWLEPDYQITVPRAFKPEGVEDGSA"
     gene            19740..22949
                     /locus_tag="QIE41_001369"
     CDS             19740..22949
                     /locus_tag="QIE41_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDS3530277.1"
                     /translation="MTYQAIFTGWDDLTIEDLLVAYRKAKADSFFENTFPVAIKFAEY
                     EQELLENLQKLLDLLQSEDGFSSNKKLIGKFRLLPKKLTTKKKHESQNGHVHFSNPKR
                     AADHLFNNFDLIPEFRIIGDFPVDSHIISALWINMVGHKFDASLDNCCYGARLKRIRN
                     DELFSNEQDNPFHISAVGSFSPYFQPYQKWRGDGLKAIRDELEKDRDIIAASLDLKSY
                     YHFIDPLAITSDDLYNTLNIKLTEDEKAFTAQLAVFLKHWSDGAAAFGKKIAYKTPVI
                     NGGLVIGLTASRIISNILLHHWDKLVIEKLSPIHYGRYVDDMFLVIRDTGTITNNHEF
                     MLLLQDRLGNDCVYLKNEQKQIWQIQQGEHFQGKTTIQLQSDKQKLFVLQGRAGIDLL
                     DSIEKEIYELSSEHRLMPSPDQLEHSTAAKVLSAAGSVGENADTLRRADGLTIRRLGW
                     SLQLRYVETLARDLPPSEWKEQREEFYQFAYNHILRADNLFAHFSYLPRLLGFAISMN
                     EWQHAEKIVLKAYESINLLASVITSGKEVNINGCKTRAVNDLWRCIKGTLSWLFVDAA
                     TRYYSPDRLFLDKRSKKEECLADTFFNHISQSLTNLKDLLDLRFDSADFYLKAPLVAR
                     ADLAKEPYKQIVKSQSAEKLVNQRDSKKEVKILKLMSDSSLIDIDVIKLFLKSTKNTR
                     LEKVAKGNRKNESYLPYIFPTRPLTPAEISELAPECVGLPSTSDKKPDERPSTIWAKY
                     TQALRGVWIKPTLLASEQDSDEATKKARPKKFIHIGTDRKHKVVVALTSIKTEEDDWA
                     KMACNKSNLSRSRYQRISELVNATLKLSPKPDYVLFPELSIPLRWVNSIADRLSSAGI
                     SLIAGTEYRHLDDNQLKSEAVLVLSDNRLGYPASVKIWQPKLEPAVGEDEALFSIYGK
                     SWDSTLNVKQRKPVYIHHGVNFGVMICSELQNSKARIRFQGALDALMVLSWNKDLDTF
                     ASLIESAALDIHAYTILVNNRKYGDSRVRSPAKEPFMRDIARVKGGDNDFVVAATLDI
                     DSLRAFQSRAKRWPKGGDKFKPLPEGFQLAKNRKKLPPK"
     gene            23110..23250
                     /locus_tag="QIE41_001370"
     CDS             23110..23250
                     /locus_tag="QIE41_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDS3530278.1"
                     /translation="MTRQRLFLCCSHGYISMVGRAGASQDAPVSDKAGKANPARFHHP
                     RD"
     gene            23296..23766
                     /locus_tag="QIE41_001371"
     CDS             23296..23766
                     /locus_tag="QIE41_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530279.1"
                     /translation="MATILTTDSDLLDLLTVPFNATTDFTVLADYCARFAETLIETDD
                     PALRLALCGRLHAALALLQPTLHDPIPPHLTESLTVDTLPATPEHFISEPDSLCEYCL
                     ALLQLLLERPAEPKMEQTLTGLLCELVWYFTEGLKAPRWLRTADGVKFISEVAA"
     gene            23763..23865
                     /locus_tag="QIE41_001372"
                     /pseudo
     CDS             23763..23865
                     /locus_tag="QIE41_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773560.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            23957..24115
                     /locus_tag="QIE41_001373"
                     /pseudo
     CDS             23957..24115
                     /locus_tag="QIE41_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24130..24205)
                     /locus_tag="QIE41_001374"
     tRNA            complement(24130..24205)
                     /locus_tag="QIE41_001374"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24170..24172),aa:Thr,seq:cgt)
     gene            complement(24320..25573)
                     /gene="proA"
                     /locus_tag="QIE41_001375"
     CDS             complement(24320..25573)
                     /gene="proA"
                     /locus_tag="QIE41_001375"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDS3530280.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESSVALHADVAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(25585..26688)
                     /gene="proB"
                     /locus_tag="QIE41_001376"
     CDS             complement(25585..26688)
                     /gene="proB"
                     /locus_tag="QIE41_001376"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDS3530281.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            26976..28031
                     /gene="phoE"
                     /locus_tag="QIE41_001377"
     CDS             26976..28031
                     /gene="phoE"
                     /locus_tag="QIE41_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDS3530282.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(28070..28471)
                     /gene="crl"
                     /locus_tag="QIE41_001378"
     CDS             complement(28070..28471)
                     /gene="crl"
                     /locus_tag="QIE41_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDS3530283.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(28529..29773)
                     /gene="frsA"
                     /locus_tag="QIE41_001379"
     CDS             complement(28529..29773)
                     /gene="frsA"
                     /locus_tag="QIE41_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDS3530284.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(29865..30323)
                     /gene="gpt"
                     /locus_tag="QIE41_001380"
     CDS             complement(29865..30323)
                     /gene="gpt"
                     /locus_tag="QIE41_001380"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDS3530285.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            30584..32041
                     /gene="pepD"
                     /locus_tag="QIE41_001381"
     CDS             30584..32041
                     /gene="pepD"
                     /locus_tag="QIE41_001381"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDS3530286.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(32098..32450)
                     /gene="prfH"
                     /locus_tag="QIE41_001382"
                     /pseudo
     CDS             complement(32098..32450)
                     /gene="prfH"
                     /locus_tag="QIE41_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(32446..32652)
                     /locus_tag="QIE41_001383"
                     /pseudo
     CDS             complement(32446..32652)
                     /locus_tag="QIE41_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(32898..33350)
                     /locus_tag="QIE41_001384"
     CDS             complement(32898..33350)
                     /locus_tag="QIE41_001384"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS3530287.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(33360..33758)
                     /gene="yafO"
                     /locus_tag="QIE41_001385"
     CDS             complement(33360..33758)
                     /gene="yafO"
                     /locus_tag="QIE41_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HDS3530288.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(33761..34054)
                     /gene="yafN"
                     /locus_tag="QIE41_001386"
     CDS             complement(33761..34054)
                     /gene="yafN"
                     /locus_tag="QIE41_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HDS3530289.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(34106..35161)
                     /gene="dinB"
                     /locus_tag="QIE41_001387"
     CDS             complement(34106..35161)
                     /gene="dinB"
                     /locus_tag="QIE41_001387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDS3530290.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(35232..36017)
                     /gene="lafU"
                     /locus_tag="QIE41_001388"
     CDS             complement(35232..36017)
                     /gene="lafU"
                     /locus_tag="QIE41_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDS3530291.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISLSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            35989..37701
                     /locus_tag="QIE41_001389"
                     /pseudo
     CDS             35989..37701
                     /locus_tag="QIE41_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            37806..38084
                     /locus_tag="QIE41_001390"
     CDS             37806..38084
                     /locus_tag="QIE41_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDS3530292.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            38077..38433
                     /locus_tag="QIE41_001391"
     CDS             38077..38433
                     /locus_tag="QIE41_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDS3530293.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
BASE COUNT         9735 a         9978 c         9748 g         8974 t
ORIGIN      
        1 ttaacctata ttgatatgtg ctacgtatct tatttacttc cgatttacta aagaaactga
       61 atgtacctgt aaaaattaca ggtttggaaa gtagtgacat ggcaaaatga ttacagcagg
      121 gactatgagg ttaaaaacca tatggaatgt caaaaccgtt ctgataaata catctggtct
      181 ccccatgacg cctacttcta taaaggacta tctgaactga ttgtggatat cgacagatta
      241 atttatctat cgctggagaa aatcagaaaa gatttcgtgt ttatcaatct caatacggat
      301 tctttaactg agtttataaa ccgtgataat gagtggttat ccgcggtaaa ggggaaacag
      361 gtcgtattga ttgcggccag aaagtcagaa gccttagcaa attattggta ttacaacagc
      421 aatattaggg gcgtggtata cgctggactg agtcgtgata ttagaaaaga actggcctat
      481 gtgattaatg gcaggttcct gagaaaagat attaagaaag ataaaatcac tgaccgggaa
      541 atggaaatta tccgcatgac ggctcaggga atgctgccta aatcgattgc cagaattgaa
      601 aattgtagtg tgaagacagt gtatacccat cggcgtaatg ctgaggccaa gctgtactca
      661 aaaatatata agttggttcc gtaaactcca ggcaagttag ttttaaaaaa tgactcacag
      721 ggacatcacg tcctcaattc aactcgggaa gaaaagcaat gaaaaaaaag gttctggcaa
      781 tagctctggt aacggtgttt accggcatgg gtgtggcgca ggctgctgac gtaacagctc
      841 aggctgtagc gatctggtca gcaacagcca aaaaagacac caccagtaag ctggttgtga
      901 cgccactcgg tagcctggcg ttccagtatg ccgaaggcat taaaggtttt aactcacaga
      961 aaggtctatt tgacgtagct atcgagggtg actcaacggc taccgccttt aaactgacct
     1021 cacgtcttat caccaacacc ttaacccagt tggatacctc aggttccaca ctgaatgtgg
     1081 gcgttgatta taacggcgcg gcagtcgaaa aaactggcga taccgtgatg atcgataccg
     1141 ccaacggcgt actgggcggc aaccttagcc cactggctaa cggttacaat gccagcaatc
     1201 gtaccaccgc acaggatggt ttcactttct ccatcatcag cggcaccacc aatggtacca
     1261 ccgcagtaac cgattacagc actctaccgg aaggcatctg gagcggcgac gttagcgtac
     1321 agttcgacgc tacctggacc agttaatctc tctgatgtac cagcaggggt acccccctgc
     1381 tttattcctg gacggaattg ttatgaaaaa gcaccttctg cctctcgctc tgctgttttc
     1441 cggaatatct ccggcccagg cgctggatgt cggcgatata tcatcgttta tgaacagtga
     1501 cagcagcacg ctgagcaaaa cgatccaaaa cagtaccgac agtggccgcc tcatcaatat
     1561 ccgtctcgaa cggctctctt caccgcttga cgacgggcag gttatcgcaa tggacaagcc
     1621 ggatgagttg ctactcactc ccgccagctt gctgctaccc gcccaagcca gcgaagtgat
     1681 ccgcttcttc tataagggac cagcagatga aaaagagcgc tactaccgca ttgtctggtt
     1741 tgatcaggcc ctcagtgatg cgcagcgcga taatgccaac cgtagcgctg tggccactgc
     1801 ttccgcccgc atcggcacca ttctggtcgt cgccccccgc caggcgaact accactttca
     1861 gtacgccaac ggctccctga caaatacagg aaatgcgacg ctgcggatcc tcgcctacgg
     1921 cccctgcctg aaagccgcca atggtaaaga gtgtaaagag aattactacc tgatgccggg
     1981 caagtcgcgt cgttttaccc gcgtggacac ggcggataac aaaggacggg ttgcactttg
     2041 gcagggtgat aagttcattc ccgtgaaata gatagctgtg cagatggata atgacaatgc
     2101 ctttacgacg gttctcccca ggactgaaag cccagtttgc cttcggcatg gtctttttgt
     2161 tcgttcagcc cgatgccagc gctgctgaca taagtgcgca gcaaataggt ggggtgatta
     2221 ttccgcaggc cttcagtcag gcgcttcagg acggcatgag cgtcccactt tatattcatc
     2281 tcgccggtag ccaggggcgc cagaacgatc agcgaatcgg cagcgctttt atctggctgg
     2341 atgatggaca gctacgcatc cggaaaatac agctggaaga gagtgaagat aacgccagtg
     2401 tcagcgaaca aactcgacag cagctgatgg ctctggcgaa cgccctgttc aatgaggccc
     2461 ttaccatccc cctgactgac aacgcgcagc tggatctcag cttgcgccaa ctgctgctgc
     2521 agctagtggt caagcgcgaa gcgctgggca ccgtactacg ctcacgtagc gaagacatcg
     2581 ggcagtccag tgttaacacc ctcagcagta atctgagcta taacttcggc gtctataaca
     2641 accagttgcg taacggcggg agcaacacat ccagctatct gtcgctgaat aacgttactg
     2701 cactgcgcga acatcacgtg gtgctcgacg gctcgctgta cgggatcggt agcggtcaac
     2761 aggacagtga attatataaa gcgatgtatg aacgcgattt tgccggtcac cgatttgccg
     2821 gtggaatgct cgacacctgg aacttgcagt ccttagggcc gatgaccgcc atttcagcag
     2881 ggaagattta cggcctttcc tggggaaacc aggccagctc caccatcttc gacagcagcc
     2941 agtcagccac gccagtgatc gcctttttac cggcggcggg cgaagtacat ctcacccgtg
     3001 atgggcggct actaagcgtt cagaacttca ctatgggcaa tcatgaagtg gatacccggg
     3061 gtctaccgta cggtatttac gatgtggaag ttgaggtcat cgttaacggt cgcgtgatca
     3121 gcaaacgcac tcagcgggtc aataagctgt ttagccgggg gcgcggcgtc ggtgcgccac
     3181 tggcgtggca gatatggggc ggtagctttc atatggatcg ctggtcggaa aacgggaaaa
     3241 agacgcgacc agctaaagag agctggctag caggtgcctc gacctccggc tcactgagta
     3301 cgcttagctg ggcggcaacg ggatatggat acgataatca ggcggtgggt gaaacccgtc
     3361 tgacgctgcc gcttgggggg gcgatcaacg ttaacctgca aaatatgctg gccagtgaca
     3421 gctcatggag cagcatcggc agcatcagcg ccactctacc gggaggcttt agttcgctgt
     3481 gggttaatca ggaaaaaacc cgcattggca atcaattgcg acgtagcgat gccgacaacc
     3541 gtgcaatcgg cggcacactc aacctgaact cactgtggtc gaagctgggt acgttcagca
     3601 tcagctacaa tgatgaccgc cgctacaaca gccattatta cacggcagat tactatcaaa
     3661 gtgtctacag cggtaccttt ggttcgcttg gcctgcgggc cggtattcag cgctataaca
     3721 acggcgacag cagcgccaat acagggaaat atatcgctct cgatctctcg ctaccactgg
     3781 gcaactggtt tagcgcaggg atgacccatc aaaacggcta caccatggca aacctgtcag
     3841 cacgcaaaca gtttgatgaa ggaaccattc gcactgttgg tgccaatctg tcacgagcca
     3901 tctccggcga taccggtgat gacaaaaccc tcagcggtgg ggcgtatgca cagttcgacg
     3961 ctcgttacgc cagcggaacg ctgaacgtca atagcgcggc ggacggctac atcaatacca
     4021 atttgaccgc caacggcagc gtcggctggc aaggtaaaaa catcgctgcc agcgggcgga
     4081 ccgatggcaa tgctggggtg atattcgaca ctggcctgga gaacgatggt cagatcagcg
     4141 ccaaaatcaa cgggcggatt ttcccgctta acggcaagcg taactatctc ccgctctctc
     4201 cctatggaag atatgaggtg gagttacaga acagcaaaaa ctcactcgac agttacgata
     4261 tcgtcagcgg tcgcaaaagc catctgactc tctatccagg caatgtcgct gtcattgagc
     4321 cagaagtgaa gcagatggtt accgtctccg gtcgtatccg tgcggaagac ggcacactgc
     4381 tggctaacgc acggattaac aaccatatcg gccgaacccg aaccgatgaa aacggcgagt
     4441 ttgtcatgga cgtggacaag aaatacccca ctatcgattt tcgctacagt ggcaataaaa
     4501 cctgcgaagt ggcactggaa ctcaaccagg cgcgcggtgc cgtttgggtc ggtgatgtgg
     4561 tctgcagcgg cctctcatcg tgggcggcgg tgacgcagac aggagaagag aatgagagtt
     4621 aacctactga tagcgatgat aatctttgcg ctaatctggc ctgcaaccgc gctcagagcg
     4681 gcagtgagca aaacaacctg ggcggatgct ccggcacgcg agtttgtgtt tgtcgaaaac
     4741 aactcagacg acaacttttt cgtcactcct ggcggggcgc tggatccgcg cctgaccggt
     4801 gccaaccgct ggaccggttt aaaatacaat ggttcaggaa ccatctatca gcaaagcctc
     4861 ggctacattg ataacggtta caacaccggc ctttatacca actggaagtt tgatatgtgg
     4921 ctggaaaatt caccagtttc atctccttta actggcttgc gctgcatcaa ctggtacgct
     4981 gggtgtaata tgaccaccag tcttatcctg ccgcaaacca ccgacgccag tggattttat
     5041 ggcgcgacgg tgaccagcgg cggcgcgaag tggatgcacg gcatgttgtc agacgcgttt
     5101 taccagtatc tgcaacaaat gcccgtcggc agcagcttta caatgaccat caatgcctgc
     5161 cagacctctg tgaactatga cgccagcagc ggcgcacgct gtaaggatca ggcctccggc
     5221 aactggtatg ttcgcaacgt cacccatacg aaagcagcaa atctacggtt gataaatacc
     5281 cactcgctgg cggaggtgtt tatcaacagc gacggagtac cgactctggg cgaagggaac
     5341 gccgactgcc ggacgcaaac catcggcagc cgttcaggat taagttgtaa gatggttaac
     5401 tataccctgc aaacaaacgg actcagcaac acctcaatcc atatattccc ggcgatcgcc
     5461 aactcgtcgt tagcctcggc cgtcggggcg tacgatatgc agttcagtct gaatggcagt
     5521 tcatggaaac cggtgagcaa tactgcctat tactacacct tcaacgagat gaagagcgca
     5581 gactcgatct atgttttctt ctcgagcaac ttctttaagc agatggtgaa cctcgggatc
     5641 agcgatatca acaccaaaga tctattcaac tttcgctttc agaacaccac atcaccggag
     5701 tctggctggt atgaattttc tacctccaac acgctgatta tcaaaccccg tgatttcagc
     5761 atcagtatta tctccgatga atatactcag acaccgtcgc gggagggata tgttggcagc
     5821 ggcgagtcgg cactcgattt cggctatatc gtaaccacca gcggtaaaac agctgccgac
     5881 gaagtgctga tcaaggtgac cggacccgcg caggtgattg gcgggcgctc ctattgtgtc
     5941 ttcagctccg atgacggtaa ggcgaaagta ccgttcccgg cgacactttc ctttattacc
     6001 cgcaacggag ctacaaaaac ctacgatgcc gggtgcgatg atagctggcg ggatatgacc
     6061 gatgcgctgt ggttgaccac accgtggact gatatctctg gcgaagtggg gcagatggat
     6121 aagaccacag tcaaattttc gattccaatg gataacgcca tttcgctgcg tacggtagat
     6181 gataacggct ggtttggcga agtcagcgct tcaggagaaa ttcatgttca ggcgacgtgg
     6241 cgtaacatta actaaggccc tgctgacagc ggtctgtatg ctggcggcac ctttgacaca
     6301 ggcgatttcg gtcggcaatc tgacattttc gctgccgtcc gagactgact ttgtcagcaa
     6361 acgtgtagtg aataacaaca aaagcgcacg gatataccgt attaccatca gtgctatcga
     6421 tagcccgggc agcagtgaat tgcgcacccg accggtggat ggtgaactgc ttttcgcccc
     6481 ccgccagctg gcgttgcagg ctggtgagag cgagtatttt aaattttact atcatggtcc
     6541 acgggataac cgcgagcgct actaccgggt ctcatttcgc gaggtcccca ctcgcaacca
     6601 gacaagacgc agcccaaccg gcggcgaggt tagcacggag ccggtggtgg tgatggatac
     6661 cattctggta gtacgacctc gtcaggttca gtttaaatgg tccttcgaca aggtgacagg
     6721 aacggtaagt aacactggca acacatggtt taagctactg attaagccag gatgtgattc
     6781 gaccgaagag gaaggcgatg cctggtacct acgtccggga gacgtggttc atcagcctga
     6841 gttacgtcag ccggggaatc attatctggt ctataacgac aaatttatta agattagcga
     6901 ttcctgtccg gctaagcccc cttcggcgga ctaagactta tccatcggtc gagggaagag
     6961 ctccacctca gagactcaaa aatcgtttaa ataatgtgga agcgatcgtc aatgtcacct
     7021 gtaacgatgg gctttctcat atgtacacca tagtgttcag gagacgcgga aaggtaccag
     7081 gcagcctgct aacgagtggt ttattcacga attagtacgt aaaatcggta acggctggaa
     7141 atcattcaat actcacacta tcgaaagttc cccagccaac cgcggtacgt tcttacatac
     7201 gatgtaccgc tgttctcttt acgatttata gctgtactgg tgaattatga gcaatctgaa
     7261 tccatgcatg acgtgtggtg cctgttgtgc atttttccgc gtctcttttt actgggccga
     7321 agccgacgat gctggcggta ccattcccgc caggctcact gaacaaatat ccccttttca
     7381 ccgatgcatg agcggtacca atcagaaaaa cccccgatgt attgcccttg caggaacacc
     7441 gggcaaaaat gcctgctgca cgatatataa aaatcgatcg tccacgtgca gagaattcgc
     7501 catgtctggt gagaacggag tcgtcaatga ggcttgcaat cgtgcaagag ctaaatacgg
     7561 gctgacaccg ttataaatat gcaataatta attgcactgc cccgccagct ataatcgcgg
     7621 ggcttcgttt ttcagggata acaaaacccg aaacttcttc catctgccag caacaatgcc
     7681 tcttcctcta tagtgttctc cggatctaca gcatcctgaa gtagcgcgat gtactcattg
     7741 gctatctgca tcagaaagtt aaaaacaatg tttttaacct ataaaaatgg cgctttattt
     7801 gcgccatttt tatcattcaa tgcattatct gtttgagcct aaagggatct cagggtctgg
     7861 ctcatgagta attctgtttc gtaaatctct gcgaataatt tcaatggacc agaaccagac
     7921 taaatgtcca aaaatttcag aaacattctc ataccaaggg agatcaaaca gaggtggcgt
     7981 cagtcccatg agagggaatg aaatcatatg aacaaaaagt tgggctaaag cacctgccag
     8041 taagccctgc cagagtttaa tttttggaaa tacttcagcg accacacaat aaccgacagc
     8101 aaacactatc gaaaagataa tgtgcgtaac accaacccag ttaaagacat gcccggcaaa
     8161 ggtataaaca gccgcattgg gatctgtcag tcccaaccaa tcccgaagaa aaatgtacgg
     8221 aggattgaga aagttacggg agcaatcaat ttggcctgca gccctgatta atgattccgg
     8281 gccacacgct gcattaaaca tatccaccgg gctacgtggc ggcaatggaa cttcagcccc
     8341 ccacttcaca aatgcggaaa caacgccagc aatcagccca atgaatgcag caagaccata
     8401 acgtctgcgg ttcggtggag tttgttcaaa tatattcata tctaccctgc ttgtaccatt
     8461 atgttataca cctcttcagg agtattcata aaacaagaca aatataaaga actgtattgt
     8521 tttgtataac aaaatagttt cctaatcgcc aatgaatata agcaccatca tttctcccta
     8581 ttgttatatt agaagtgaca acgatttgca gggtgatgct gagctgacaa tcacaggttt
     8641 ccttatcggt ttttgcatcg cacaactaaa gcaataaact agctcccgtc atcggcgaat
     8701 atgaattaat tagccgggct catagctgca ttcgcgtttt aagaataatc ctcctgctct
     8761 caccgactat gcttaacgtt taaaaaagca tcagcactct cgcaacgcac tcttattttc
     8821 ccctttagaa taccggaggc ctggtatgag caaccaaggc gaatatcccg aaaataatcg
     8881 ggttgggaag cacgagccgc acgattttag tttgacccgg cgcgatctga ttaaagtgag
     8941 cgccgcaacg gcggcgaccg ccgtggttta tcctcattct acgcttgcgg caagcgttcc
     9001 ggcagctaca cccgcgccag agataatgcc cctgacactt aaggtgaacg gcaaaaccga
     9061 gcagttggac gttgataccc gaaccacgct actggacgct ttgcgtgaaa atctgcatct
     9121 gatcggtacc aagaaaggtt gcgatcacgg gcagtgcgga gcctgtaccg tgctggtcaa
     9181 tggtcgcagg cttaatgcct gcctgacgct tgcagtcatg catcaggggg acgagatcac
     9241 caccattgaa ggcctgggtt caccagagaa tcttcacccc atgcaggcgg cctttatcaa
     9301 gcatgatggc ttccagtgcg gctactgcac ctccgggcaa atttgctcat cagtagcggt
     9361 gctaaaagag attcaggaca gcattcccag tcacgtcacg gtcgatttgg tttcccctcc
     9421 agaaagaact gccgatgaga tccgtgaacg tatgagcggc aacatctgtc gctgtggtgc
     9481 atacgctaac atccttgccg ccattgaaga tgctgcgggg gagataaaat catgaaggcg
     9541 tttacctatg aacgagtgaa caccccggcc gaggcggcac ttagcgctca gcgcgtaccc
     9601 ggcgcaaaat ttatcgctgg cgggaccaat ctgctggacc tgatgaagct ggaaattgaa
     9661 acgccaaccc accttatcga tgtgaacggg ctcgggctcg ataagattga agtgaccgac
     9721 gcgggtgggc tgcgcatcgg cgcactggta cggaacaccg acctggcagc tcacgagcgc
     9781 gtgcgtcgtg attacgcggt actctcccgc gccctgctcg ctggcgcgtc tggtcagtta
     9841 cgtaatcagg caaccaccgc aggtaatctg ctccagcgca cgcgctgccc ctatttttac
     9901 gacaccaatc agccctgcaa taagcgccta cccgggagcg gctgcgcggc acttgaaggc
     9961 tttagccgtc agcacgcggt ggtaggcgta agcgaagcct gcattgccac ccatccgagc
    10021 gatatggcgg tcgcaatgcg gttgctggat gcggtggtgg aaaccatcac gccggaggga
    10081 aagactcgca gtatcacact ggctgatttt tatcaccctc cgggaaaaac gccgcacatt
    10141 gaaaccgccc tgcttcccgg tgagcttatc gttgcggtga cgttacctcc gccgctcggc
    10201 ggaaaacata tctaccgcaa ggtgcgcgat cgcgcctcct acgcctttgc cctggtatcg
    10261 gtcgcggcga ttattcagcc tgacggcagc gggcgcgtcg cgctgggcgg agtagcacat
    10321 aagccctggc gcattgaggc tgcggatgct cagctatccc agggggcgca ggccgtgtat
    10381 gacgcgctgt tcgccagcgc ccatcccacc gctgaaaaca cctttaaact cctgttggcg
    10441 aagcgaacgc ttgcctccgt actggctgaa gcgagggcac aggcatgaaa tttgataaac
    10501 ccgcagggga aaacccgatc gatcagctga aggttgtcgg tcgtccccat gaccgcatcg
    10561 acggaccgct gaaaactacc ggcacggcac gctacgccta cgaatggtat gaagaagctc
    10621 ccaacgccgc ctatggttat atcgtcggtt ccgccattgc caaaggatgc ctcaccgccc
    10681 tcgatacgga cgccgcgcaa aaagcgccgg gcgtactggc tgtcattacc gccagtaacg
    10741 ccggggcact cggcaaaggc gacaaaaaca ccgccaggct gttaggaggc cccactattg
    10801 agcactatca tcaggccatt gcgctggtag tggccgagac cttcgaacag gcgcgagcgg
    10861 cggcctcgct ggtgcaggcg cactatcgcc gtaataaagg agcttactcc ctggcggacg
    10921 agaaacaggc cgtcagtcag ccgccggaag acacgcccga taaaaatgtt ggtgactttg
    10981 acggggcttt cacctccgct gcggtgaaga ttgatgctac ctacacgacc ccggaccaga
    11041 gccatatggc gatggagccg catgcctcga tggccgtctg ggatggaaat aagcttaccc
    11101 tctggacctc aaatcagatg attgactggt gccgcaccga tctggcaaaa acgctgaaag
    11161 tgcccgtgga gaatgtgcgt attatctccc cgtatatcgg tggagggttt ggcggcaagc
    11221 tgttcctgag aagcgatgcg ctgctggcgg ccctcgccgc ccgtgcggtg aaacgtccgg
    11281 ttaaagtgat gctcccccgc cccactattc ccaataacac cacgcaccgc cccgccaccc
    11341 ttcagcacct gcgtatcggt gccgaccaga gcgggaaaat caccgctatc tcacatgaaa
    11401 gctggtccgg aaacctgccc ggtggcacgc cggaaacggc ggtacagcaa agcgaattac
    11461 tctacgcagg ggcaaaccgt catacaggcc tgcggctcgc cacgcttgat ttgccggaag
    11521 ggaacgccat gcgtgcgccc ggcgaagccc ccggtctgat ggcgctcgaa atcgcgatcg
    11581 acgaactggc ggaaaaagcg ggcatcgatc ccgtcgagtt tcgcatcctg aatgacactc
    11641 aggttgaccc cgccgacccg acgcgccgct tctctcgccg tcagcttatc gagtgcttgc
    11701 gcaccggagc ggataaattt ggctggaagc agcgcaacgc cacacccgga caggtacgcg
    11761 acggggagtg gctagtcggc cacggtgttg cggcgggctt tcgcaataat ctgctggaaa
    11821 aatcgggtgc gcgggttcac ctagaacaaa acggcaccgt taccgtggaa acggacatga
    11881 ccgatattgg caccggcagc tacacaattc tggctcagac ggcagcggaa atgcttggcg
    11941 taccgctgga gcaggttgcg gttcacctcg gcgattccag tttcccggtt tctgcgggtt
    12001 ctggtggaca atggggcgcg aatacctcca cctccggcgt ttacgccgcc tgtgtgaaac
    12061 ttcgcgaaat gattgcctcg gcagtcgggt ttgatcctgg gcagtcgcag tttgccgacg
    12121 gcaagattac caacggtacc cgaagcgcca cgctacatga ggccaccgca ggcggcaggc
    12181 tgacagcgga agagagcatt gaattcggaa cactgagcaa ggagtaccag cagtcgacct
    12241 ttgccgggca ttttgtggag gtcggcgtgc atagcgcgac gggagaagtt cgggtccggc
    12301 gtatgctcgc tgtgtgtgct gcaggacgca tcctgaatcc gaaaactgca cgcagccagg
    12361 tcattggcgc gatgactatg ggcatgggcg cggcactgat ggaggagctg gcggtagatg
    12421 accgtttggg ctacttcgtt aatcacgata tggcggggta tgaggtgccg gttcatgcgg
    12481 atatcccaaa acaggaggtg attttcctgg atgataccga ccccatctcc tccccgatga
    12541 aggccaaagg tgtcggtgag ctgggcctgt gcggcgtgag cgcggctatc gccaacgcgg
    12601 tgtataacgc caccggtatt cgggtacggg attatcccat cactctggat aagctgctcg
    12661 ataagctgcc ggatgtggtt taaggaggaa caatgtcata ctcgcttttt gacaaagacg
    12721 agcactggca taagccagag caggcgtttc tcaccgatga ccaccgggcc attctgcgct
    12781 tcgccgtaga ggcgctaatg tccagtaaag gagcggtgct ggtgacgctg gtggaaatac
    12841 gcggcggcgc ggcgcgcccg ctcggggcgc agatggtggt gcgcgaagat ggtcattact
    12901 gcggttttgt ctctggcggc tgcgtggagg ccgctgccgc ttttgaagcg ctggagatga
    12961 tgggctcagg ccgcgatcgc gaaattcgct atggtgaagg ttcgccgtgg tttgacatcg
    13021 ttctgccctg cggcggtggg atcacgctga cgctccataa actacgctcg gcacaacctc
    13081 tgctcgccgt gctgaaccgc ctggaacaga gaaaaccggc gggtctgcgc tacgatccgc
    13141 aagcacaatc gttggtgtgc ctgcccacgc aaacccggac gggctggaat ctcaatggct
    13201 ttgaggtggg gttcagacca tgcgtcaggc tgatgattta cggacgttct cttgaggcgc
    13261 aggcaaccgc gagtcttgca gcagccacag gctatgacag ccatatcttc gatctttttc
    13321 cggcctcagc cagcgctcag atcgataccg atacggcggt cattttgctg tgccatgatc
    13381 tcaaccggga actgccagtg ttgcaggccg cgcgagaagc aaaacccttt tatctcggcg
    13441 cattgggcag ctatcgaacc cacactttac gtctgcaaaa gctccacgag ctgggatggt
    13501 ccagggagga gacagagcaa atccgggcac ctgtcgggat attccccaaa gcccgggatg
    13561 cgcatactct ggccctttcc gtactggcag aagtcgccgc tgtacgtctc cgtcaggagg
    13621 attcatgcct gcccccatcg tcctgatcct cgcggcgggg cgtggggagc gcttcctcac
    13681 ctccggggga aatacccata aattaagaga aaaatctgca gtagccgttt aagaaaatca
    13741 cctaaatcgt ttaaaagagc gcccaaccca tagtgggcgc ttatcgcctt gtgaaactta
    13801 ttcagcaacg cgcgcctcgc cggactaaag cgcaccggcc ttctggcgtg cggtaacaaa
    13861 ggtcagtacg ccagcgcaaa ggagcagcac gctactcata gcaaacgtgc tctgccagcc
    13921 aaggcggtca aacacaatgc ctcctgccgt tgatccaagg gcgatggcga gctgaatcac
    13981 cgcgaccatg agcccgcctc cagcttcggc gttgtcaggc aacgtacgcg caatccatgt
    14041 ccaccatccg gttggcgcgg cggtggccag catgccccag agtcctaaca gcagcgagac
    14101 cgtccagacg tgatgcccgg taagcatcag accgatggca atgacggcca tcagtccggg
    14161 tatggccatc agcgtggggt agaattttct gttgagaaat gccgaaacaa ccagcgtgcc
    14221 aataaaaccg gccacgccga taatcagcaa aatcgacgac aagcctaagg aattaacccg
    14281 tgtcaccgac tccagaaacg ggcgcacata ggtgaatagc gcaaactgcc ccatgaaaaa
    14341 caggccacaa gctatcatgc cgaccacaac catgcggcgg ctaaataagc ggaataccgc
    14401 gccacatgaa gtgtggcttt tattcgcatc catgcttggc aggctgatgc actgccagat
    14461 aaaggcgaca accgcgatgg gcaccaggca caagaaagcg cctcgccatc cgaccgtagc
    14521 ccccagatag cttcccagcg gtgcggccac gaccgtcgcc agcgcattac cggcgttgaa
    14581 aattgccagc gcacgcgtga cctgatgctg cggcaccaga cgaatcgccg ttgccgctga
    14641 catcgaccag aatccgccaa tagccacacc aatcatcgca cgaccgacca tatacatcag
    14701 ataactggaa gccagcgcaa taataagccc cgatacggcc atcagaaccg tcatccccag
    14761 ctgcaggaac ttacgattca tctttccagc tagcgttgaa agcgtcaggc tggtcagaac
    14821 cgccagcgcg ccggagatag caatcccctg ccctgccagc ccctccgtca cgcctaaatc
    14881 gcgggtaatc ggcgtgagca ggctgacggg cataaattca gaagcaatca gcacaaacac
    14941 gcacagggtc atggcaaaaa taccgcccca gtatgcgcgc tgatgatgag gtgatagtgt
    15001 ttggcttagt gtcgacatag tgaaatttca caaagatcag gataaaagcc accagcctat
    15061 ggccggtggc tttgcatcaa ttcaggagag gttttatttc agtttggtct ggaaaaattg
    15121 ttcgaacttc gcgaaaggta tttttccggc cacgttatcg tacaggtcca cgtgatttgc
    15181 ccctggtacc accaccaact ctttgtcttt actgccaacc gccttgtaag catcttcagc
    15241 aaaatagcgt gaatgtgctt tctcaccggt cacaatcagc gtcggaatgg tgatttcact
    15301 ggcgtagctc aacagcggca tattcataaa tgacagcggc atcgttgccg tccacgcccc
    15361 ggtagagtta acggaacgtt cgtggaagcc gcgcggcatg cgatagtagt caaagaactc
    15421 tttcagtacc ggatgaggat ttgccggcag cgtttctggc agaatgcgct cgcccgcact
    15481 gaccttgccg ttgctgtcga cattaatatc atgaccaccg tgagcgaacg ttccgctctg
    15541 cgcatccttc cagcgctgtt cattcagata ttgcagaaca gcacgacggt cggcggtaga
    15601 ataacggtct ttgccatccc ccacaccatg gcccatcgcc cggctcatgt catacatcac
    15661 gctggtagct accgctttaa cgcgggtatc catcgccgcg tcgttcagcg ccatgccgcc
    15721 ccagccacaa ataccgagca gcccgatacg gttgcgatct acctcttttt gcagccctaa
    15781 gaaatccact gccgcgctga aatcttcagt gttgatatcc ggtgaggcga cgtttcgcgg
    15841 atagccgccg ctttccccgg tgtaagaagg atcgaacgcc agggtaacaa accctcgttc
    15901 cgccagcgtc tgcgcataca ggccgctgga ttgctctttc accgcgccaa atggcccact
    15961 gaccgcaatc gccgccagct tgcgatcgcc acggtcttta ggcaggtaaa gatcgcccac
    16021 taaggtgatg ccatatcggt tcgggaacga gactttacgg tgatcgactt tctggctctc
    16081 ggcgaacgtt ttatcccatt tatctgtcat ggacacaggg gcattaggat tggttgaatc
    16141 agcataactc attgtcgtga ctccacttaa tgatgcgaaa agcagcatgg tcggcatcgc
    16201 ggtttttagc gtgcgggtga aggctttcat aaaaatcccc ataaaaaaca acagagagat
    16261 acaggtttcc gacggacaac gctgcggtgc tttactttga tggggtcatt atagtgagca
    16321 gaattagtgc tgattagggg gcgattaatg ctaagattaa taccatattg ttataaatta
    16381 atgtggagat tgccggcatg aaggggggat gtttcaatgg cgaaaaggga gaactacaac
    16441 gagctgtacc tgtttatgca ggtggtgcgg gaaggcagct ttaccgcagc ggctcagcgg
    16501 ctgggtcttg ctcagtcagg agtcagccgt tctgttcgcg agcttgaaga aaggctgggt
    16561 gtccagctgc tagtgcgcac cacgcgtaaa ctgtcgctga cacaagcagg cgagcagctc
    16621 taccagaaga cggcatctgg atttgaaatg ttagatcttg ggcttgccac gctggcgcat
    16681 tatcgagaga cgccctccgg cacggtgcgt atcaatgcca gccagcacgc gattgataaa
    16741 tgcctgctgc caaagctggc ggtatttaaa cagagatatc ctgatatccg gcttgaactg
    16801 ataaatgaga gccggttcgt cgatatcatc gaggaaagat tcgatgcagg tgtccgcctg
    16861 gggcctgaag tcggccaggg tatggtcgcg gtacgcatca cgcccgatat ggagatggca
    16921 attgtgggga ctccggaaca ttttcgtcgc tatggttttc cgcaaacccc ggcggattta
    16981 aaggcgcatc cctgtatcgc ctatcagttt gccgacggca gcgtatacca gtgggaactc
    17041 aatcaggatg ataaaaaaat cactcatcgc cctgaagggc aatgggccct atccgacagc
    17101 tatatggaag cagaagccgc ccggctgggc ctcggattgg cctatgttcc tgttgaactg
    17161 gtcgcggatg atttagaaca cggtaagctt atcagggtgt tgcagcgtta cagcctgcga
    17221 atggagggat tgtttctcta ttaccctcat cgcaacgtct cccccgccct gcggatggtc
    17281 attgatacat tgaaaatctg aacgttaaca acgaccgact cttcactgtg caaaattgtg
    17341 tcaggtagtc cctctgcgtt caattccagg gtctgctttg agcgagaaag aggggactat
    17401 ccagcaatgg agatcaccca aaagaaaaaa ccacctggct ccctcgacaa ccttcatccg
    17461 ggcatttagc ctcaatcaac atttttatat ctgtcaggtt acttcattat gaaaactgat
    17521 catcatcgtt gtttttgttg caatagtaga atacgcgtat ttattcctcc atctctatta
    17581 ataacggcaa taaactgttc acttcactga tatttaaaat atgtatcctt ttcaagctgt
    17641 tcagcggcgt aagaaaaagt attaattatg aattcgatga tttaaccata tctgtctgat
    17701 tggtaatcat ccgtgattaa tgctggtcta ttgattacgc cagaaccctc tctagcttat
    17761 tcaccgccgc cacatacttc gccggatttt tatctttaag caccgccaga ttgcgttgct
    17821 tccagcgaat agccggaaga tgctgaattt caggatagcg ataccgcgac cagtcagggc
    17881 tgttttgctt aaaactgagc aagaactcgc gatccagatc cgtaagatgt gacttcaacg
    17941 cctgcggcag caaagtcgtt actgacagaa gcgattccag cgacgtttcc tgttgcgtca
    18001 tccctgaaaa ttcctgcgcg tacaacgtgg cgatccgctc tgtatcccag tttggagcca
    18061 gcaactcggc gatggggcgg ggatggcctg ccagataaca aagaaaaccg tcaaagatct
    18121 cccgcatcag tcccggtttc tccagcatat tcagcacatc aaacagatcg cgcgggtgct
    18181 gacgatcaag cgcagcgcag attttaccgc cataaagatc cgccagcgag acgacactca
    18241 tggtggtgaa gccatactcc atttccacac gttcgcacac aggcatttct gccggaggca
    18301 acaacagccc gcgccagacc gggctaacct cgatcttaat ctttgcatca gcggtattca
    18361 caatgatgcg cttttcatcc gctttatttt cctgccgaat cgcggtgatc cccggtctgc
    18421 cgttcagcga tgcagtaatc ctcttcaaag cgtcgttgat agccgccaaa tcagtatcgc
    18481 gatccgcaaa gctcttgtac gccagatcaa tatccaccga cagacgggga aaatcctgca
    18541 caaacaaatt gattgcggtg ccgcctttca acgcgaaaca ggactccttc gccacatacg
    18601 gcagagcggc catcaacagt ttgacctgcc gggcatagct atcacgcaga tccatcttcg
    18661 actccttccg gtttaaatgc gcgtggaaca gtaatttgat aatccggctc cagccagccg
    18721 ccgggtatga tttgccgctt tcccgtacct gtttctatac cgctttgatc aatatactga
    18781 aaataagggt gtccattacg ccgggcaagg taaagcatca cccggttggt tttaaccgaa
    18841 tggctggcgc gaagcagtgc ctgcaatttg cgtgggctga gaagattcaa cccctgaaac
    18901 agagcatcgg catgttcgaa cgagattaac gaaggaacgc cgcaggcgat ctcatacgcg
    18961 gccagttccg gaacactgcc tgctaactgg cggtcgccaa ttttcagttc cgtagtaaat
    19021 ggcgttacat cagcggtaag gcctgacgta tacagcgtga caaacgttgc ggattgttca
    19081 aaggcagaaa accatcccgg cagtttgacg taagccggaa gcgcgagcca gacctgcggc
    19141 ttaccctgtt cggtatagtg cgcatgaccc tgaagcgcaa ggctggttag ccccgccacc
    19201 cgaacaggct tttcccactg cgattgcagg cagtaaaccg catccaccca atccggttca
    19261 cgacccgtcc ggcagtatac gccgtgagcg atccgcctga gccagccatt cttcacatag
    19321 agataagaga gcttacgatc tatgccgttg cgggtaagcc atgactggag cagcacctgg
    19381 ccgggtagcg tgtgctgcat cagccagttt agatagtttg gcaactgtcg cctctgaata
    19441 gacattagta caaatattta aaccagtgta gattcaggga tacgccacag caagaaatag
    19501 tttacttatt cctcattttg tcgactaaaa atcgacatta aacaaaaaat tcaaacttaa
    19561 tcactttcgg gaaaaatgtg acaaatatat gctcggactg gttgcgggga gcgtgtaaca
    19621 tggatacaaa tcaaaattat tgccagcctc actgatggat tactggtgtc aagagccccc
    19681 cttcgggcat gaaacggctg gctaattctg tacagactgt aatctaagga cgataacgca
    19741 tgacatatca ggcaattttc actggctggg atgatctgac gattgaagac cttctggtcg
    19801 cttaccggaa agcaaaagcc gatagcttct ttgagaatac atttcctgtt gctatcaaat
    19861 ttgccgagta tgagcaggaa ttacttgaaa acctgcaaaa actcttagat cttttgcaga
    19921 gcgaagatgg attcagtagc aataagaagt tgattggcaa atttcgtttg ttaccgaaaa
    19981 aattaaccac aaagaaaaaa catgaatccc aaaatggaca cgtccacttt tctaatccta
    20041 aacgagcagc cgaccattta tttaataatt ttgatctgat accagagttt cgtattattg
    20101 gtgacttccc ggttgatagt cacattatct ctgcactatg gattaacatg gtcgggcata
    20161 aatttgatgc cagcttagat aactgttgct atggcgcgcg gctaaagcgt attcgtaatg
    20221 atgaattatt tagcaatgag caggataatc cattccatat cagtgccgtg ggttctttta
    20281 gcccctactt ccagccctac caaaaatggc gtggtgatgg cttaaaagct atacgtgacg
    20341 agttggaaaa agatcgtgac attatcgccg cctcactgga tttaaaaagt tactatcatt
    20401 ttattgatcc actggctata acctctgatg atctctataa cacactaaac ataaaactga
    20461 ctgaggatga aaaagcgttt actgcacagt tagcagtatt cttaaagcac tggtctgacg
    20521 gcgcagcggc atttggaaag aaaatagcgt acaaaacacc tgttattaat ggtggtctgg
    20581 tcattggatt aacagccagt cggatcattt caaatatatt gctacaccat tgggataaat
    20641 tagtcattga aaaactatca ccaattcact acggtcgtta tgtcgatgat atgttccttg
    20701 taatacgcga tacagggaca attactaata atcacgaatt tatgttattg ctgcaagata
    20761 ggcttggcaa tgattgcgtt tatttgaaaa acgagcaaaa acaaatatgg caaatacagc
    20821 agggcgagca tttccagggt aagaccacca tccagttaca atccgataag caaaaacttt
    20881 tcgtgcttca agggagggct ggaatagacc tgctcgacag tatcgaaaag gagatctacg
    20941 agctttctag tgaacaccgc ttgatgcctt caccggatca actggaacac tccaccgcag
    21001 ctaaagtcct ttccgctgcc ggtagtgtag gtgaaaatgc cgatactctg cgccgtgcgg
    21061 atggattaac cattcgtcgt ttgggctggt cactgcaatt acgctacgtt gaaacactgg
    21121 cacgagatct gcctccaagt gaatggaaag aacagcggga agagttttat cagtttgcct
    21181 acaaccatat tcttagggct gataatctat ttgcacattt tagttatctg ccaaggctgc
    21241 ttggctttgc tatcagtatg aatgaatggc agcacgcgga aaaaattgta cttaaagctt
    21301 acgaatccat caacctgttg gcatcggtga ttacttcagg taaggaagtg aatataaatg
    21361 gttgcaaaac tcgagcagta aatgatcttt ggcgctgtat aaaaggcaca ttaagctggc
    21421 tatttgttga tgcagcgaca cgatattaca gtcctgacag attatttctt gataaacgtt
    21481 caaagaaaga agagtgcctt gcggatacat tttttaatca tatttcacaa agtctgacga
    21541 atctaaagga tttactggat cttcgctttg attcagcaga tttttattta aaagcgccat
    21601 tggtagctcg agctgattta gcaaaggaac cttataaaca gatcgtaaag agtcagtcgg
    21661 cagaaaaact tgttaatcag cgtgatagta aaaaagaagt taaaatactg aaattaatga
    21721 gcgactcatc gcttattgat attgacgtta ttaagctatt tttgaaatca accaagaata
    21781 cccgactgga aaaagtggct aaaggaaatc gtaagaacga aagttaccta ccttacattt
    21841 tccctacacg tcctttaaca cccgctgaaa tatcagaact ggcccccgaa tgtgttggat
    21901 taccctccac atccgacaaa aaaccagatg agagaccgtc caccatttgg gcaaaatata
    21961 ctcaagcatt acgcggagta tggatcaaac cgacgttgct agcatcggag caggactcag
    22021 atgaagcgac aaaaaaagct cggcctaaga aattcattca tattggcaca gacaggaaac
    22081 ataaagttgt cgttgcgcta accagcatta aaacagagga ggacgactgg gctaaaatgg
    22141 cctgcaataa atctaacttg tcccgttcaa ggtaccagcg gatttctgaa ctggttaatg
    22201 caacattgaa actatctcct aaacctgatt atgttttatt ccctgagctt tcaatcccgt
    22261 tacgctgggt taacagtatt gctgatcgtt tgagttcggc gggtatcagt ctaattgcgg
    22321 gaacagaata ccgccactta gacgataatc aactgaagag tgaggccgta cttgtccttt
    22381 cagataacag actcggctat ccagcgagtg tcaaaatatg gcaacccaag ctggaacccg
    22441 ccgtaggtga agatgaggca ttattttcaa tttatggtaa gtcttgggat tcgacactta
    22501 atgttaaaca acgtaagccg gtatatattc atcacggcgt caattttggc gttatgattt
    22561 gctctgaact ccagaatagt aaagcgagga tccgttttca gggcgcactc gatgcattaa
    22621 tggtattgag ctggaataaa gatctagata cgtttgcatc gttgattgaa tcagcagcgc
    22681 tggatattca tgcctatact attttagtga ataaccgaaa atacggcgat agtcgcgtac
    22741 gttccccggc aaaagaaccc tttatgcgtg atattgctcg tgtgaagggc ggtgataatg
    22801 actttgtggt cgctgcaacg ctggatatcg actcgttaag ggcatttcag agcagggcaa
    22861 aacgctggcc taaaggcggc gataaattca aaccgttacc tgaaggattc cagttggcaa
    22921 agaaccgcaa aaagctaccg ccaaaataag aaactgattt tcgctattaa taatcagggt
    22981 atttttgcgt gagatgttgg taaacatgat gtagcccttg ccactcatga ccaatcgcag
    23041 tatctttctc ccgcgcctgc aaaatcaggc gtcgggatta gcctcctgaa gaaatcttat
    23101 cggcgacaca tgacgcgcca gcgtcttttt ttgtgttgtt cgcacggtta catctcaatg
    23161 gtgggccggg cgggggcgtc gcaagacgcg ccagtgtccg ataaggctgg taaggctaac
    23221 cctgcccggt tccaccaccc gcgagattag cctctccggt ggtggcttga acacccactt
    23281 atcggaggtt gccctatggc tacaatcctc accactgaca gcgatttact cgatctattg
    23341 accgtcccct tcaatgccac cacggatttc actgtccttg ctgactactg cgcccgcttt
    23401 gccgaaaccc tgattgaaac cgacgatcca gcgctacgac tggcgctctg cgggcggctt
    23461 cacgcggctc ttgcgctact gcaacccaca ctacacgacc ccatcccacc gcatcttacc
    23521 gaaagcctga ccgtggatac ccttcccgcc acaccagagc actttatttc cgaacctgat
    23581 tccttgtgcg aatactgcct cgcactactc cagcttctgc tggaacgtcc ggcggaacca
    23641 aaaatggagc aaaccctgac cgggctgctg tgtgagctgg tctggtattt cacggaagga
    23701 ctcaaagccc cacgctggct gcgtaccgcc gacggcgtaa aatttatcag cgaggtggcc
    23761 gcatgatgca acaggactgg cattccgccg acattattgc ggtgctgaaa aagcggggaa
    23821 cgcctctggc ggcacagaga aaaactcatt gcggaagcgc tgtaaatatc gccagaaatc
    23881 atctggcctt caaggtactt tgatagcaaa ggcaagcagg ctggttcagt ccagtgtttc
    23941 tcttcttgca ttttcagaaa tgggaggctg ggagtctatc gaaatggttc gtcgatatgc
    24001 tcacctcgcg cctaatcacc ttaccgaaca cgcacggcaa atagactcag tcctgaaccc
    24061 atcggtccca aatttgtccc agtcaaaaaa taaggaaggt actaatgatg tgtaacttat
    24121 tgattttaat ggtgccgata agaggagtcg aacctccgac cttcgcatta cgaatgcgct
    24181 gctctaccaa ctgagctata tcggccctga aaggacatgt tcacgaacgt gaatcacggt
    24241 ggacaaggtt aaaactaacc gggcgatgcg tcaatggcct tgtgaatcaa atggctactt
    24301 ttgcatcacc cggttttatt tacgcacgaa tggtgtaatc accaatgccg atccacttgt
    24361 aagtggtcag tgcttccagc cccattgggc cacgcgcgtg gagtttttgt gtgcttaccg
    24421 ccacttccgc acccagacca aactggccgc catcggtaaa acgcgtagag gcgttaacgt
    24481 aaacagcgga cgaatccact tcgttaacaa aacgctgggc gttgcgcata tcgcgggtca
    24541 ggatcgcatc ggagtgttgt gtgccgtgtt cacgaatatg ggcgatggca tcgtcaagat
    24601 cgctgacgat tttaacgttc aaatctaatg acagaaactc atcgtcatac tcttcggctt
    24661 taacagccac caccttcgcg gggcctgcct gcaactgcgc cagcgcagct acatctgcgt
    24721 gtaatgccac gctgctttcc gccatttgct tgcttaatgc gggcaggaag ctatcggcga
    24781 tgtttttatt taccagcaac gtttctaccg tattacatgt gctcggacgc tgagtttttg
    24841 cgttgacgat tactttcagg gcttcagcga tctctgcact ttcatcaacg taaatatggc
    24901 atacgcctat accgcctgtg atcaccggga ttgtcgactg ttcgcggcac agcttatgca
    24961 ggccagcgcc tccacgcggg atcagcatgt cgatgtattt atccatacgc agcatttcac
    25021 tgaccagcgc acggtcagga ttatcaatcg cctgcacggc acccgccggt aagccgcagg
    25081 atttcagggc gtcctgaatc accgctaccg ttgcagcgtt agtgcgacag gtttctttgc
    25141 caccacgcag gatcaccgcg ttaccggttt tcaggcacag cgaagcgaca tcaaccgtca
    25201 cgttcgggcg cgcttcataa atcacgccaa taacccccag cggtacgcga cgacgctcaa
    25261 gacgcaggcc gctgtccagt acgccgccat cgattacctg ccccaccgga tcggcgagat
    25321 tacacacctg acgcacatcg tcggcgatgc ctttcagccg tgcgggcgtc agtgccagac
    25381 ggtcaagcat cgcttcgcta aggccattgg cacgcgcgtc agcaacatcc tgggcgttag
    25441 cgttgaggat gatttcgctt tgtgcttcca gttcatcggc gattttttcc agcacgcgat
    25501 ttttttcgcg gctggagagt tgcgctaatt tatacgaggc ttgcttcgcg gcaatgccca
    25561 tttgttccag catcagcctg ctccttaacg ggtaatcatg tcatcacggt gaacggcaac
    25621 cgggccatat tcatatccca gtattgcatc aatttcttgc gagtggtgtc cggcaatgcg
    25681 gcgtaatgca tcgctgttgt aacgactgac gccgtgagcg atatcgcgac cttcgaggtt
    25741 gcaaatgcgg atgacttcac cacgcgagaa attgccagtc acgcttttaa tgcctttcgg
    25801 caacagggaa ctgccgcgtt caagaatggc ggcagttgcc ccttcatcta ccgtgatttc
    25861 acccgccggc ggcgcaccga aaatccagcg tttacggttt tcaagcggag tcgcctgggc
    25921 atggaacagc gtaccgacgg aaatgccttc catcacatca ccaataacgc ccggcttgct
    25981 gcccgcggca ataatggtgt cgatacccgc acggcaagcc acgtccgcgg cctgcaattt
    26041 ggtactcatg ccgccagttc cgaggcctga aacgctgtca ccggcaatcg cgcgcagtgc
    26101 gtcatcaatg ccgtaaacat ctttaatcag ttctgcctgc ggattgctgc gcgggtcagc
    26161 ggtatacaaa cctttttgat cggtcagcag caacagttta tcggcacccg ccagaatcgc
    26221 cgccagcgca gaaaggttat cgttatcgcc gaccttaatc tctgccgtag cgacagcatc
    26281 gttctcattg attaccggaa cgatattgtt atcgagcaac gcacgcaggg tgtcgcgggc
    26341 gttcaggaag cgttcacggt cttccatatc agcacgagtc agcagcattt gcccgacgtg
    26401 aatgccataa atcgaaaaca gctgttccca cagttgaatc agtcgactct gccctaccgc
    26461 cgccagcagt tgtttcgagg cgatagtcgc tggcagttcc gggtaaccca ggtgctcacg
    26521 tccggcggcg atcgcgcccg acgtcacaat aacaatccga tgcccggcgg catgtaactg
    26581 cgcgcactgg cgaacaagtt caacgatatg ggcacggttc aggcggcgcg atccgcctgt
    26641 tagcacactg gtgccgagtt ttaccaccag cgtctggctg tcactcatga ttctctgcca
    26701 ttcaatttta ggaaaaatga tatcaaacga acgttttagc aggactgtcg tcggttgcca
    26761 accatctgcg agcaaagcat ggcgttttgt tgcgcgggat cagcaagcgt agcggcagtt
    26821 gtttacgctt ttattacaga tttaataaat taccacattt taagaatatt attaatctgt
    26881 aatatatctt taacaatctc aggttaaaaa ctttcctgtt ttcaacggga ctctcccgct
    26941 gaatattcgc gcgttaatta aaatcaggaa tgaaaatgaa aaagagcact ctggcattag
    27001 tggtgatggg cattgtggca tctgcatccg tacaggccgc agaaatatat aacaaagacg
    27061 gtaataaact ggatgtctat ggcaaagtta aagccatgca ttatatgagt gataacgaca
    27121 gtaaagatgg cgaccagagt tatatccgtt ttggttttaa aggcgaaaca caaattaacg
    27181 atcaactgac gggttatggt cgttgggaag cagagtttgc cggtaataaa gcggagagtg
    27241 atactgcaca gcaaaaaacg cgtctcgctt ttgccgggtt gaaatataaa gatttgggtt
    27301 ctttcgatta tggtcgtaac ctgggcgcgc tgtatgacgt ggaagcctgg accgatatgt
    27361 tcccggaatt tggtggcgac tcctcggcgc agaccgacaa ctttatgacc aaacgcgcca
    27421 gcggtctggc gacgtatcgg aacaccgact tcttcggcgt tatcgatggc ctgaacttaa
    27481 ccctgcaata tcaagggaaa aacgaaaacc gcgacgttaa aaagcaaaac ggcgatggct
    27541 tcggtacgtc attgacatat gactttggtg gcagcgattt cgccatcagt ggggcctata
    27601 ccaactccga tcgcactaac gagcagaacc tgcaaagccg tggcacaggc aagcgtgcag
    27661 aagcatgggc aacaggtctg aaatacgatg ccaataatat ttatctggca actttctatt
    27721 ctgaaacacg caaaatgacg ccaataactg gcggctttgc caataagaca cagaactttg
    27781 aagcggtcgc ccaataccag tttgactttg gtctgcgtcc gtcgctgggt tatgtcttat
    27841 cgaaagggaa agatattgaa ggcatcggtg atgaagatct ggtcaattat atcgacgtcg
    27901 gtgctacata ttatttcaac aaaaatatgt cagcgtttgt tgattataaa atcaaccaac
    27961 tggatagcga taataagctg aacatcaata atgatgatat tgtcgcggtt ggcatgacct
    28021 atcagtttta atggatattg ccggatgtga tgcatccggc acatttcact cacgccgtta
    28081 acttcaccgg ttcatcacga aaatcatccg ccggttccag cttcagattc agcgtcgcca
    28141 gcagctcacg cagcttctcg tgaaattcac gcagggtgtg ctccagtcgt tcaaccactt
    28201 cggcatcttt tatctgaaca ctcgtccagt cgcctgcttt atcgaacaga ccaaactggt
    28261 aactgtaggt aaaacgggat tcctgcgctt ccagttccat ccaccagccc cagaactcac
    28321 gcacttccgg tgccggtttc acgttgacgc atacagccag acaatcgaaa aagaatcgat
    28381 tatcttcgca cttaccttca cgaatatacg ggcctagtgc ggtaaatttt ttgatcaatc
    28441 tgctcttcgg gtgtccactc ggtaacgtca ttgcaatctc ctgttgtgat gcaactgttt
    28501 taccaaattg gcaaaattta gcaaattttt aacacaatcg tttttcaatc cagtctgtta
    28561 tttcctgaag acctttgtca aaattccgat acaccgggtt aaatgggatc tctaacaatt
    28621 taccgtcagc agatgatgag gtgattaagc gtgagtcctc ttccgggctg aacggatcgt
    28681 tcttccagta gcctgataac attggcgttg ggcagcgacg tcccagcaat ccttgcactt
    28741 ttaatgaata gcgattcagc tccacgcgca acgcttcgtc ggaagcatca tgcatcccca
    28801 aacggctcgc cagaacgtca agatacattt ccggcacctg ttgctggcac ttaaaatcac
    28861 tcaacagggt atgaactacc ggaccaagac aggcaaccgc tttcagacgc ggcgattcaa
    28921 ggtatgccag acgcacggca acgttagcgc cgaaacggaa accaaaggcc gcgacgcgag
    28981 tgtgatctac ccacggtacg ttaggcagcg cctttaagac gtgctgatgc aacaggctgg
    29041 agtcctgggt gagcttccat tttgaagaaa agcccaccga cggcatatca atagtcagca
    29101 tcgcaatgcc gcgcggcgca aaataacgtt catacaggct gtaatagtcc gtctgcatcg
    29161 catccagacc accacacatt aatactgtcg ggaacgggcc atcgcctttc ggcatatgca
    29221 aaaagccggt gatgggcgca ccgccgggta cggtaaactc catctgccgc atggtgcccg
    29281 gcagacgctg agcggcctct tcataggcgc ggtttgacaa agcctgcgct tgctcggcca
    29341 ggtcatctcc tttcagatga ggataggcgg caatgttgta caacgtagcc gcatgcagcc
    29401 agtgacgacc actgagttgc ggatcttctt ccgcacaggc tttttgttgc cacaccatcg
    29461 cctgggtggc ccactcataa atccagttgc caccacggta gccaatcacc gtatcgtata
    29521 aatcatcgtc ggtacgttcg gcatcgctca tcacaatacg tgcctggacg tcgaggattt
    29581 cacgtgggtc aatgccgcgc cagatccaca tcagacggtt aatcatgcga taccagtgag
    29641 ggatggtttt accatcaagg gccgactgca caggcggttg tgcgccgtga ttaaagcggc
    29701 ggactagcgt cgaggtttca ggatgtttaa agcggggttt gaacagggtt tcgctcaggt
    29761 ttgcctgtgt catggatgca gcctccagaa tacttactgg aaactattgt aacccgcctg
    29821 aagttaaaaa gaacaacgcc cggcagtgcc aggcgttgaa aagattagcg accggagatt
    29881 ggcgggacga atacgacgcc catatcccac ggttgttcga tccaggtatc ttgcgggata
    29941 tcaacaacat agtcatcaac cagcggacga ccagccggtt ttgcgaagat ggtgacaaag
    30001 tgcgcttttg gatacatttc acgaatcgca accgcagtgc caccggtatc caccaggtca
    30061 tcaataacga tgaagccttc gccatcgcct tctgcgcgtt tcagcacttt aagctcgcgc
    30121 tggttgtcgt gatcgtagct ggaaatacaa acggtatcga catgacgaat acccagttca
    30181 cgcgccagta acgcacccgg taccagaccg ccacggctta cggcaataat gcctttccat
    30241 tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag
    30301 gtgacgatgt atttttcgct catgtgaagt gtcccagcct gtttatctac ggcttaaaaa
    30361 gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt
    30421 atttcacatg agtccgcgtc tttttacgca ctgcctctcc ctgacgcggg ataaagtggt
    30481 attctcaaac atatctcgca agcctgtctt gtgttgacaa cattttctgc taaccctgtg
    30541 acctgcaata ctgttttgcg ggtgatcgac aaggagactt aacgtgtctg aactgtctca
    30601 attatctcca cagccgctgt gggatatttt tgccaaaatc tgttctattc ctcacccgtc
    30661 ctatcatgaa gagcaactcg ctgaatacat tgttggttgg gcaaaagaga aaggtttcca
    30721 tgtcgaacgc gatcaggtag gtaatatcct gattcgtaaa cctgctaccg caggtatgga
    30781 aaatcgtaaa ccggtcgtct tgcaggccca cctcgatatg gtgccacaga aaaataacga
    30841 caccgtgcat gacttcacta aagatcctat ccagccttat attgatggcg aatgggttaa
    30901 agcgcgcggc accacgctgg gtgcggataa cggcattggt atggcgtctg cgctggcggt
    30961 tctggctgac gaaaacgtgg ttcacggccc gctggaagtg ctgctgacca tgaccgaaga
    31021 agccggtatg gatggtgctt ttggcttaca gagcaactgg ttgcaggctg atattctgat
    31081 taacaccgac tccgaagagg aaggtgaaat ctacatgggt tgcgcggggg gtatcgactt
    31141 cacctccaac ctgcatttag atcgtgaagc agttccagct ggttttgaaa ccttcaagtt
    31201 aaccttaaaa ggtctaaaag gcggtcactc cggcggtgaa atccacgttg gcctgggtaa
    31261 tgccaacaaa ctgctggtgc gcttcctggc gggtcatgca gaagagctgg acctgcgcct
    31321 tatcgatttc aacggtggca cactgcgtaa cgccatcccg cgtgaagcct ttgcgaccat
    31381 tgctgtcgca gctgataaag tcgacgccct gaaatctctg gtgaatacct atcaggaaat
    31441 cctgaaaaac gagctggcag agaaagagaa aaatctggca ttgctgctgg actctgttgc
    31501 gaacgataaa gctgccctga ttgcgaaatc tcgcgatacc tttattcgtc tgctgaacgc
    31561 caccccgaac ggtgtgatcc gcaactccga cgtggcaaaa ggtgtggtcg aaacctccct
    31621 gaacgtcggt gtggtgacca tgactgacaa taacgtagaa attcactgcc tgatccgttc
    31681 tctgatcgac agcggtaaag actacgtggt gagcatgctg gattcgctgg gtaaactggc
    31741 tggcgcgaaa accgaagcga aaggcgcata tcctggctgg cagccggacg ctaattctcc
    31801 ggtgatgcat ctggtacgtg aaacctatca gcgactgttc aacaagacgc cgaacatcca
    31861 gattattcac gcgggcctgg aatgtggtct gtttaaaaaa ccgtatccgg aaatggacat
    31921 ggtttctatc gggccaacaa tcaccggtcc acactctccg gatgagcaag ttcacatcga
    31981 aagcgtaggt cattactgga cactgctgac tgaactgctg aaagaaattc cggcgaagta
    32041 attatttgat ttgctgccgg atggcgttta atcgccttcc ggcagtttca tccttcatta
    32101 tccttcgata aaagccatcc ctgtaaatgt ccgtctcggg ttgccacgtt caatctggtg
    32161 atggaacatt cgccgctgcg atttcagcac cgcgctattt tcctgttgct gttgttccag
    32221 cttccaggca atcagcaatc gcgccagccg cttgttagca tgcccagaaa caagtttttg
    32281 cgcccatgat gaggccgata cggactcgga caaatccact gaatagtgcc gcaccaactt
    32341 tcgcttaatg cccatgcgtt atcgccatcc agagaaatca gcgccgaacg cagcgtgtca
    32401 gagcagcggc ccgtttctgt ttccagcacc gttaccgcga cgtcttgccg ggtaaaaacg
    32461 accaacagag aataacgctg cgccaatcgg gtagccgcgt ccggggtgta aatctggcat
    32521 ccctactacg cgctgcatgt tgggtaaatt tgccgtggtg tgtaattgtt ggatcgctaa
    32581 actctcgatc cacaggtcat cagatgcgat ggtaaatacc gcatcggata agggacgaat
    32641 atatttgccc atgtatcatt ccatttatac tcgtcatact tcaagttgca tgtactgcga
    32701 ctcgaattat ttagagtata taaatataaa aatcaggaaa tggcagacga atccagggca
    32761 agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc attgataaag gatttgtcat
    32821 gatttacctc cttgcagttt gtggaataag aaaacgcgca catactaggc gggcgttatt
    32881 ttcattgcaa gctggattta atgttgcggt ttatatcgca tataaaaatt agtaaaccac
    32941 gctccccggc attcaacgcg ctgctgctta actgtctgaa aaccataacg ttcaaaaaag
    33001 ggttttgcgg ttatgctggc atctaccgtt agttcggatt cagacttaat caaaggtttt
    33061 aacaaagcgc tggcaacccc acggcgggtg tattcagggt caacaaataa catatcgata
    33121 taatgttcaa cgcaggtaat aaagccgacc agttttgcat taatgactgc aacccgcact
    33181 tgtgatttcg cgagtttctc cttccagcga gattcgtcaa tctgcgccca ggcggcaatt
    33241 tgttgtggtg aataatgctg actggcggtc atcgtaaccg ctctaatgaa aatagcgcat
    33301 agttgctgaa aatcgccagg ctgataatct cttatttgta tgttattcat aatataaatt
    33361 caaaaacgca tgcgaaacgc ttctgccatt tttccaattt tatgcatttg gttgttatct
    33421 cgagccagtt tatgaggttc aggcttcaga atggcaatga gcaaccatgc ttgctcatca
    33481 aacgcaccct gacaatatac caaatgcgct tcgtcattcg ttctgctgaa ttggcgcaac
    33541 tgtggcggaa atggattatt cacatttgcc agatgaatat gagcaactcg ctcaaatttg
    33601 attaatggcc aggtaaacga gtcgtcgtag agtgcatcgc gaccaaatat atctggcaaa
    33661 acaccgtcac gcttatagga aataaaatcc gccgttaacg catcaagttc ctctgctgta
    33721 agttgcaggc gaataagttt tgttttgaat acccgcatcc ttattcctta aagtcattga
    33781 aatcatcatc cgtcatatca ttaagatatt gttcagcgcg gcgtgtaatt tcctctaatc
    33841 ttgcggcact cggacggaag cgcgccttca ccagtttttt ctcctcttgt tctgcaagca
    33901 tgtccattaa cgcttcgaat gcgctggcac ttaagagata tcctgcgggg cgattattag
    33961 aaagaaccgc aaccggttga tcaataaagt attttgctgg atttttacgt aactcagtga
    34021 tattgaccga tttttcagcg agaattcgat gcatacagtg ataccctcat aataatgcac
    34081 accagaatat acataatagt atacatcata atcccagcac cagttgtctt tccatttgcg
    34141 ggtcaagcaa cgtcacatgc agccccacca gccgcacacc gcgcccgccg cgacgttcat
    34201 cccaggtttt acgcgcggtg gcaattaagt cagctttatt cagccgcggc cagacgtgct
    34261 cctgagtggt ttgctgaaaa tcatcaaact ttaatttcac cccctggcga gcaatcagta
    34321 aatcaggttt tacttttgcc agacggcgtt caagttccgg gtacagccgc tcgataatcg
    34381 cttcacattc agaccagtga tgaatatctt ccgccatcgt gcgttccacg ccgacggatt
    34441 ttcgcaaccg ttcgctgtta acgtcgcgct cgtcaatccc ctgactacgc tcccacaaaa
    34501 tgcggccaaa tttgccaaaa cgtttgagca gagtcaccag atcacacttt tgcacatcac
    34561 cgcaggtccg cagccccatc gcttccagtt ttgccgccga gactttgccg acgccgggga
    34621 tttttgccag cggtaaggtt tgtaaaaatg tcggaacttc tgccggcgta atcacaaact
    34681 ggccgttggg tttattcatg tcggaggcga ttttggcgag aaactttact ggtgccacgc
    34741 ccgcagacgc cgtcagttgc agctcgctga aaatcgtctg gcggatttcc tgggcgatga
    34801 gggtcgcaga accgtggcaa tggacgctat cggtgacatc gagataggcc tcatccagtg
    34861 acaacggttc aatgcgcgag gtgtagcgcg agaagatttc acggatatga tttgaggctt
    34921 ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg tgggcataat ttgagcgcca
    34981 tcccagtagg catagcgcta cgtacgccaa atttacgcgc gggataattg gcggtgctga
    35041 tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg gatatcgcgc agggcgggat
    35101 tgtcgcgcat ctccactgcg gcgaaaaagc agtccatatc cacatgaatg attttacgca
    35161 ttgctcacct ctcaacactg gtaaagtata cagtgatttc agggtttgag aaatgcgtaa
    35221 agattcagca tctagcgcgt tcgctgcgaa agcacctgct gcttatccag cttttgcacc
    35281 agcggctgca ccactttatc cccatgctga ccaaagtatt gatacagcgt atcggacgca
    35341 cttttggtca gcaccataat ctcaatgcgt cggttgcccg cgctttgcgg atttttcgcg
    35401 tccagcagca tctggtccgc catcgcgctc acctgcatca ctttatcttc cggcattccg
    35461 gcctcttcca gcacccgacg agccgaaagc gcgcgatcgc ccgaaaggtt ccagttgttg
    35521 tagatattgt ttttgtaggc catcgcatcg gtatgcccgg taataataat tttgttatcg
    35581 agcgagtcga acactggcgc gagctccacc agcaaagttt taaagaacgg cataatctgt
    35641 gcgctgccgc gttcaaacat attgcggttc tggtcatctt taatcagcac gcgcagtccc
    35701 tggggaacaa tctccatctc cagattcgct tccatatgtg catcacgggc aatggtgttg
    35761 atactggtcg ccagctcgcc aagctcagta gcggatttct tcttcagcag cgccgttttc
    35821 tcattgacgt cacgcgcttt tttctccgtc tcttccggca ccgccacggt ggcaggtttt
    35881 ggcggatgag acaggctgat tttatttaac ggcgacaacc cgccgccatt gaaaatcgac
    35941 tgcccgtgca gcgcggcgat aatgctttca cgctcggatt tactgacgct gttgacaatc
    36001 aactttatcg tcgtcaccaa aggggccgag cgtatttccg aggtttctgc ccgctttacc
    36061 ctggacgcga tgcccggcaa acagatggcg attgacgccg atcttaacgc cgggttgatc
    36121 aaccaggcgc aggcgcaaac ccggcgtaaa gatgttgcca gcgaggccga tttctacggc
    36181 gcaatggacg gggcatcgaa gtttgtgcgc ggggatgcca tcgccgggat gatgattctg
    36241 gcgatcaacc tgatcggcgg cgtctgtatc gggatcttca aatacaacct gagcgccgat
    36301 gccgccttcc agcaatatgt gctgatgacc atcggcgacg gcctggtggc gcagatcccg
    36361 tccctgctgc tttccaccgc ggcggcgatt atcgtcaccc gcgtcagcga caacggcgat
    36421 attgcccatg acgtgcgtaa ccagttgctg gcaagcccgt cggtgctcta caccgccacc
    36481 gggatcatgt ttgtgctggc ggttgtgccg gggatgccgc atctgccgtt tttgctgttc
    36541 agcgccctgc ttggttttac cggctggcgg atgagcaaac gcccgcaggc ggcggaggcg
    36601 gaggagaaaa gcctcgaaac gctgacccgc accatcaccg aaaccagcga gcaacaggtc
    36661 agttgggaga ctattccgct gatcgaaccc atcagtttaa gcctcggtta caaactggtg
    36721 gcgctggtcg ataaagcgca gggtaaccca ctcacccagc gtattcgcgg cgtgcggcaa
    36781 gtgatatccg acggcaacgg cgtgctgctg ccggagatcc gtattcggga aaacttccgc
    36841 ctcaagccca gccagtacgc catattcatc aacggcatta aggccgatga agcggatatt
    36901 ccggcggata aactgatggc cctgccctcc agcgaaacct acggcgagat tgacggcgtg
    36961 ctggggaacg acccggcgta cgggatgccg gtgacctgga tccagcccgc gcagaaagcg
    37021 aaagcgctga atatggggta tcaggtgatc gacagcgcca gcgtgatcgc tacccatgtg
    37081 aacaagattg tgcgcagcta tattcctgat ttgtttaact atgatgacat cacgcagttg
    37141 cataaccgtt tgtcgtcgat ggcaccgcgc ctggcggaag atttgagcgc ggcgctcaat
    37201 tacagccaat tgctgaaagt gtaccgggcg ctgctgaccg aaggcgtttc cctgcgcgat
    37261 attgtcacca tcgccaccgt gctggtcgcc agtagcacag tgaccaaaga tcatatcctg
    37321 ctggcggccg atgtgcgcct ggcattgcgg cgcagcatta cccatccgtt cgttcgcaag
    37381 caggagctga cggtgtatac gctgaataat gagctggaaa atctgctcac caacgtggtg
    37441 aatcaggcgc aacagggcgg gaaagtgatg ctcgacagcg tgccggtcga tccgaatatg
    37501 ctcaaccagt tccagagtac gatgccgcag gtaaaagagc agatgaaagc ggcggggaaa
    37561 gacccggtgc tgctggtgcc gccgcagttg cgtcctttgc tggcgcgtta tgcaaggttg
    37621 tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc cggacgagct ggagttgaag
    37681 attatggggg cgttgagttg attgaggaat cttttccatg ctttgcagca cctgacacca
    37741 ttaattgacg tcacactgca aagagactac gatgacacca tacaatgacg tcatggattg
    37801 atagtatgat taaggtcagc gcgctaggct ctcgtcatag aaaaacattc gacgatgtaa
    37861 tgacggagcc gccaaaatct ggaattaaat gggatgatgt tgttgcactc attaaagctg
    37921 tgggtggaac aataaaaaat aacaacggct ccagacgaaa atttcagatt ggaacgacca
    37981 aattcagcac acatgagcca catccaaaga atgtgatgga taaaggcgca gtagcaggtc
    38041 ttaaggagtg gtttgttaac tgtgtagggg tagattatga ataaagcatc gaacattatc
    38101 acaattaaag gtcaaccagc cgcagtgaca tttgaagccg atatcaacgc atttcgcggc
    38161 aagttcctca atgtaaacgg ttattgtgac tttgtagcca caagtattga ggcattatat
    38221 cgtgaaggcg aaagcgcgct ggatgagtgg atggcggatt gtgaagagga cggtattgcc
    38281 ccttttcgtg aagaagaaga gcaaaaacgc ctgacgcttc gtgttcctca tcacattgat
    38341 tcgcggctga ccatcgtcgc cagacaacat tccatttcaa aaaaccagct cattgttgat
    38401 gtcttagaac gtgagttttc agcccagatg taaac
//
LOCUS       DAOTRK010000027        37914 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.027, whole genome shotgun sequence.
ACCESSION   DAOTRK010000027 DAOTRK010000000
VERSION     DAOTRK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.027"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            135..806
                     /locus_tag="QIE41_001392"
     CDS             135..806
                     /locus_tag="QIE41_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDS3530294.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            816..2012
                     /locus_tag="QIE41_001393"
     CDS             816..2012
                     /locus_tag="QIE41_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDS3530295.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSATGT
                     EKLSEA"
     gene            1990..2571
                     /locus_tag="QIE41_001394"
     CDS             1990..2571
                     /locus_tag="QIE41_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDS3530296.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQTYLGAQVRTSNHRLDQQPVSVRTPEGIIATECDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2573..3346
                     /locus_tag="QIE41_001395"
     CDS             2573..3346
                     /locus_tag="QIE41_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDS3530297.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3534..3809
                     /gene="frmR"
                     /locus_tag="QIE41_001396"
     CDS             3534..3809
                     /gene="frmR"
                     /locus_tag="QIE41_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDS3530298.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3844..4953
                     /gene="frmA"
                     /locus_tag="QIE41_001397"
     CDS             3844..4953
                     /gene="frmA"
                     /locus_tag="QIE41_001397"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDS3530299.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5046..5879
                     /gene="fghA"
                     /locus_tag="QIE41_001398"
     CDS             5046..5879
                     /gene="fghA"
                     /locus_tag="QIE41_001398"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDS3530300.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAASEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6005..6544)
                     /locus_tag="QIE41_001399"
     CDS             complement(6005..6544)
                     /locus_tag="QIE41_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDS3530301.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6646..7857)
                     /gene="mhpT"
                     /locus_tag="QIE41_001400"
     CDS             complement(6646..7857)
                     /gene="mhpT"
                     /locus_tag="QIE41_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDS3530302.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILISSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8033..9046)
                     /gene="dmpG"
                     /locus_tag="QIE41_001401"
     CDS             complement(8033..9046)
                     /gene="dmpG"
                     /locus_tag="QIE41_001401"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDS3530303.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGTHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9043..9993)
                     /gene="mhpF"
                     /locus_tag="QIE41_001402"
     CDS             complement(9043..9993)
                     /gene="mhpF"
                     /locus_tag="QIE41_001402"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDS3530304.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVQYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9990..10799)
                     /gene="mhpD"
                     /locus_tag="QIE41_001403"
     CDS             complement(9990..10799)
                     /gene="mhpD"
                     /locus_tag="QIE41_001403"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDS3530305.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10809..11675)
                     /gene="mhpC"
                     /locus_tag="QIE41_001404"
     CDS             complement(10809..11675)
                     /gene="mhpC"
                     /locus_tag="QIE41_001404"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDS3530306.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11693..12637)
                     /gene="mhpB"
                     /locus_tag="QIE41_001405"
     CDS             complement(11693..12637)
                     /gene="mhpB"
                     /locus_tag="QIE41_001405"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDS3530307.1"
                     /translation="MYAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12639..14303)
                     /locus_tag="QIE41_001406"
     CDS             complement(12639..14303)
                     /locus_tag="QIE41_001406"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDS3530308.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14494..15327
                     /gene="mhpR"
                     /locus_tag="QIE41_001407"
     CDS             14494..15327
                     /gene="mhpR"
                     /locus_tag="QIE41_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDS3530309.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLH
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15404..16486
                     /gene="lacI"
                     /locus_tag="QIE41_001408"
     CDS             15404..16486
                     /gene="lacI"
                     /locus_tag="QIE41_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDS3530310.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16609..19683
                     /gene="lacZ"
                     /locus_tag="QIE41_001409"
     CDS             16609..19683
                     /gene="lacZ"
                     /locus_tag="QIE41_001409"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDS3530311.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDECWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYVEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPVLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEVPLDVAPQDKQLIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADILADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSVQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19735..20988
                     /gene="lacY"
                     /locus_tag="QIE41_001410"
     CDS             19735..20988
                     /gene="lacY"
                     /locus_tag="QIE41_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDS3530312.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21054..21665
                     /gene="lacA"
                     /locus_tag="QIE41_001411"
     CDS             21054..21665
                     /gene="lacA"
                     /locus_tag="QIE41_001411"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDS3530313.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVEPSV"
     gene            complement(21753..22934)
                     /gene="cynX"
                     /locus_tag="QIE41_001412"
     CDS             complement(21753..22934)
                     /gene="cynX"
                     /locus_tag="QIE41_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDS3530314.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAMTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22940..23410)
                     /gene="cynS"
                     /locus_tag="QIE41_001413"
     CDS             complement(22940..23410)
                     /gene="cynS"
                     /locus_tag="QIE41_001413"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDS3530315.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDERIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23441..24100)
                     /gene="cynT"
                     /locus_tag="QIE41_001414"
     CDS             complement(23441..24100)
                     /gene="cynT"
                     /locus_tag="QIE41_001414"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDS3530316.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24210..25109
                     /gene="cynR"
                     /locus_tag="QIE41_001415"
     CDS             24210..25109
                     /gene="cynR"
                     /locus_tag="QIE41_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDS3530317.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECAVVGGNESR"
     gene            complement(25149..26432)
                     /gene="codA"
                     /locus_tag="QIE41_001416"
     CDS             complement(25149..26432)
                     /gene="codA"
                     /locus_tag="QIE41_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDS3530318.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLDQPEAIDYKR"
     gene            complement(26422..27681)
                     /gene="codB"
                     /locus_tag="QIE41_001417"
     CDS             complement(26422..27681)
                     /gene="codB"
                     /locus_tag="QIE41_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDS3530319.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAISGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVIPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(27918..29804)
                     /gene="prpE"
                     /locus_tag="QIE41_001418"
     CDS             complement(27918..29804)
                     /gene="prpE"
                     /locus_tag="QIE41_001418"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDS3530320.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29844..31295)
                     /gene="prpD"
                     /locus_tag="QIE41_001419"
     CDS             complement(29844..31295)
                     /gene="prpD"
                     /locus_tag="QIE41_001419"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDS3530321.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGVMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQVAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILDVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(31329..32498)
                     /gene="prpC"
                     /locus_tag="QIE41_001420"
     CDS             complement(31329..32498)
                     /gene="prpC"
                     /locus_tag="QIE41_001420"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDS3530322.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32543..33433)
                     /gene="prpB"
                     /locus_tag="QIE41_001421"
     CDS             complement(32543..33433)
                     /gene="prpB"
                     /locus_tag="QIE41_001421"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDS3530323.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQAK"
     gene            33672..35258
                     /gene="prpR"
                     /locus_tag="QIE41_001422"
     CDS             33672..35258
                     /gene="prpR"
                     /locus_tag="QIE41_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDS3530324.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLASERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVTDILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPSPSLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35359..35634)
                     /locus_tag="QIE41_001423"
     CDS             complement(35359..35634)
                     /locus_tag="QIE41_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDS3530325.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35781..36452
                     /locus_tag="QIE41_001424"
     CDS             35781..36452
                     /locus_tag="QIE41_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDS3530326.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36469..36714)
                     /locus_tag="QIE41_001425"
     CDS             complement(36469..36714)
                     /locus_tag="QIE41_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530327.1"
                     /translation="MAVQLFITLLNQIPLLSSLQSETLPLFGYSGRGRAMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(37127..>37914)
                     /locus_tag="QIE41_001426"
     CDS             complement(37127..>37914)
                     /locus_tag="QIE41_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530328.1"
                     /translation="LMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYP
                     YDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVF
                     RILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEHHMP
                     LLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLL
                     LSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT         8923 a        10253 c         9849 g         8889 t
ORIGIN      
        1 acctgaaagc cccttatttt tcagctatca gtaattttta acacttaaaa attagcatct
       61 gtctgtgcca gagattacgt ctctgcgggc atcgttgtgc ttgtagtata ataaataaaa
      121 tggagtgtca ttaagtggat aaggttttag attcagccat cctttcttcg gcaaataaaa
      181 gaaagggtat ccttgctatt ggcgcacatc ctgatgatat agaattgggc tgtggcgcgt
      241 cgcttgctcg tcttgcgcaa aaaggaattt atatcgcagc cgtggtgatg actaccggca
      301 actctggcac agatggaata atcgatcgcc atgaagaatc gcgcaacgcc ctaaagatat
      361 tagggtgcca ccaaactatt catcttaatt ttgctgacac ccgcgcgcat ttacagctca
      421 atgatatgat ttccgccctg gaagatatca ttaaaaatca aattccttct gatgttgaaa
      481 tcatgcgggt atataccatg catgatgccg accgccatca ggatcatctc gctgtttatc
      541 aagcttcaat ggttgcctgc cgcactattc cacaaattct cggctacgaa accccgagta
      601 cctggctttc atttatgcct caggtttttg aatccgttaa agaagaatat ttcacagtca
      661 aacttgctgc attaaaaaaa cataaaagcc aggaacgacg cgattatatg cgtcatgatc
      721 gcctgcgtgc agttgcacaa tttcgcgggc aacaggtcaa tagcgatctg ggtgaaggct
      781 ttgtaattca taaaatgatt ctttgagcaa attgcatgaa aacctggata tttatatgta
      841 tggccgtagc aatattgcta tggttcctga gtacgttaag gcgtaagccc agccaaaaaa
      901 aaggttgtat tgacgccatt atacctgcgt ataacgaagg cccgtgtctg gcacagtcac
      961 tggataatct actgcgtaac ccttattttt gccgggtaat ttgcgttaac gacggctcca
     1021 cggacaatac cgaagcggtc atggcggaag tcaaacgcaa atggggcgac cgctttattg
     1081 ccgtcacgca aaaaaatacc ggtaaaggtg gtgcgctgat gaatggcctc aattacgcca
     1141 cctgcgacca ggttttttta agtgatgccg acacctatgt tccgcccgat caagacggaa
     1201 tgggctatat gcttgcagaa atagagcgcg gtgctgatgc cgtaggcggc attccctcta
     1261 ctgcgttgaa aggcgcgggt ctgttaccgc acatccgcgc gaccgtaaag ttgccgatga
     1321 ttgttatgaa gcgcacgcta cagcagctcc tgggcggcgc accgtttatt atcagcggtg
     1381 cctgcgggat gttccgtact gatgtattgc gtaagttcgg tttctctgat cgtactaaag
     1441 tcgaagacct tgatctcacc tggacattgg tggcaaacgg ctatcgtatt cggcaggcga
     1501 atcgctgcat cgtataccca caggaatgca acagcccgcg tgaggaatgg cgtcgctggc
     1561 ggcgttggat tgtgggctac gcggtctgta tgcgcctgca taaaagactt ttatttagcc
     1621 gcttcggtat cttcagtata tttcctatgc tgttggttgt gctttatggc gttgggattt
     1681 atctcactac ctggtttaat gaattcatca ccaccgggcc gcatggtgtg gtgttggcaa
     1741 tgtttccgct tatctgggtc ggcgtagttt gtgttattgg tgcttttagc gcctggtttc
     1801 atcgttgctg gttgttggtg cctttagcgc cgctttccgt tgtgtatgta ttattagctt
     1861 atgccatctg gattatttat ggacttattg ccttttttac tggacgcgaa cctcagcgcg
     1921 acaaacccac ccgctattcc gcactggtgg aagcgtcaac cgcttattcc caaccttctg
     1981 ctacaggaac tgaaaaacta tctgaagctt aatgttaaag agaaaaatat tcagattgca
     2041 gaccaggtaa ttattgatga aactgcaggt gaagttgtta tcggcgcgaa tacccgtatt
     2101 tgtcacggtg ccgttattca gggtccggta gtgattggcg caaactgcct gataggtaat
     2161 tatgcgttta ttcgtcctgg cacaataatc agcaatggcg taaaaattgg ttttgccacc
     2221 gaaattaaaa atgcggttat tgaagcggaa gcaacgattg gtccgcaatg ctttatcgcc
     2281 gactcggtag ttgcaaacca gacatatttg ggcgcacaag tacgtaccag taatcatcgt
     2341 ctggatcaac agcccgtatc tgttcgaact ccagagggaa ttatcgctac cgaatgcgat
     2401 aaattaggtt gttatatcgg gcagcgttca cgccttggcg tacaagttat tattttgcct
     2461 gggcgaatta tttccccgaa cacgcaactt ggcccgcgcg tgattgtaga acgtaattta
     2521 cctaccggaa cttattcact ccgacaagaa cttatccgta caggagatta aaatgataaa
     2581 acggacgtta ttagcggcag caatttttag cacattgccc gcttatgccg ggttaacttc
     2641 cattaccgcg ggctacgatt ttaccgatta ttctggcgat catggcaacc gtaatttagc
     2701 gtatgctgaa ctggtggcga aagttgaaaa cgcaacgctg ctttttaatc tttcccaggg
     2761 gcgtcgtgat tatgaaactg aacatttcaa tgccactcgc ggtcagggtg cggtctggta
     2821 taaatggaat aactggctga caacccgaac gggtattgcc tttgcggata atacgccggt
     2881 ctttgcccgc caggattttc gtcaggatat taacctggca ctgttgccaa aaacgctttt
     2941 cacgaccggt tatcgctaca cgaaatatta cgatgatgtc gaagtcgatg cctggcaagg
     3001 cggcgtatca ctctatactg gcccggtgat caccagctat cgctataccc attatgactc
     3061 cagcgatgca ggtggtagtt acagcaatat gatttccgtg cgtctgaatg acccgcgcgg
     3121 cactggttat acgcaactat ggctaagccg cggaacaggt gcttacacct atgactggac
     3181 gccagaaaca cgctacggca gcatgaagag cgtcagtttg caacgtattc aaccgctgac
     3241 tgagcaactt aatctcgggc tgacggcagg taaagtgtgg tacgacaccc caaccgatga
     3301 ttataacggt ctgcaacttg cagcccatct gacctggaaa ttctgattcc ttctgccgcc
     3361 cgctatccgg ggcggccttc cctgccgatt agccccaccc cctttcctct ttgttttccg
     3421 accacattca ccggataaat tttattctcc agtgttatat actatagggg ggtatgcatt
     3481 gacatataga atacccccct atagtatatt gcatgcagat gatgaggtgc gaaatgccca
     3541 gtactccgga agagaagaaa aaggtcctta ctcgagttcg tcgtattcgg gggcagattg
     3601 atgctctgga acggtcgctg gagggtgatg ccgaatgccg tgccatactc caacagatcg
     3661 ctgccgttcg gggcgcggct aacgggctga tggcagaagt gcttgaaagc catatccggg
     3721 aaacgtttga ccgaaatgac tgctacagcc gcgaagtcag ccaatccgtt gacgacacta
     3781 ttgaactggt tcgtgcctat cttaaatagc tgaatctatt accatattga ggaagagcga
     3841 gagatgaaat cacgtgctgc cgttgcattt gctcccggta aaccgctgga aatcgttgaa
     3901 attgacgttg caccaccgaa aaaaggtgaa gtgctgatta aagtcaccca taccggcgtt
     3961 tgccataccg acgcatttac cctctccggc gatgacccgg aaggtgtatt cccggtagtt
     4021 ctcggtcacg aaggggccgg cgttgtggtt gaagtcggtg aaggcgtaac cagcgtcaaa
     4081 cctggcgacc atgtgatccc gctttacacc gcagagtgcg gcgagtgtga gttctgtcgt
     4141 tccggcaaaa ccaacctctg tgttgcggtt cgcgaaaccc agggtaaagg cctgatgcca
     4201 gacggcacca cccgtttttc ttacaacggg cagccgcttt atcactacat gggatgctct
     4261 acattcagtg aatacaccgt ggtcgcggaa gtgtctctgg ccaaaattaa tccagaagca
     4321 aaccacgaac acgtctgcct gctgggctgt ggcgtgacga ccggtattgg cgcggtacac
     4381 aacacggcta aagtccagcc aggtgattct gttgccgtat ttggtcttgg cgcgattggt
     4441 ctggcagttg ttcagggcgc gcgtcaggcg aaagcgggac ggattatcgc tattgatacg
     4501 aacccgaaga aattcgatct ggcgcgtcgc ttcggtgcta ccgactgtat taacccgaat
     4561 gactacgaca aaccgattaa agatgtcctg ctggatatca acaaatgggg tatcgaccat
     4621 acctttgaat gcatcggtaa cgtcaacgtg atgcgtgcgg cgctggaaag tgcgcaccgc
     4681 ggctggggtc agtcggtgat catcggggtc gcgggttccg gtcaggaaat ctccacccgt
     4741 ccattccagt tggtcactgg tcgcgtatgg aaaggttccg cgtttggcgg cgtgaaaggt
     4801 cgttcccagt taccgggtat ggttgaagat gcgatgaaag gtgatatcga tctggaaccg
     4861 tttgtcacgc ataccatgag cctggatgaa attaatgacg ccttcgacct gatgcatgaa
     4921 ggcaaatcca ttcgaaccgt aattcgttac tgattgcccg caggtttacc ccgtccagtt
     4981 caggcggggt tcttaatact ctccccgggc agccgtccgg gggattaacc gtgagataat
     5041 gactgatgga actcattgaa aaacatgcca gctttggcgg ctggcaaaat gtgtatcggc
     5101 attattcccc atcactgaaa tgtgaaatga atgtcggcgt ctatctccca ccaaaagccg
     5161 caagtgaaaa attgccggtg ttgtactggc tttcagggct gacttgcaac gagcagaatt
     5221 tcattactaa atcggggatg cagcgttacg cggcggagca caacattatt gttgttgcgc
     5281 cagacaccag tccgcgaggc agtcatgtcg cagatgctga ccgttacgat ctcgggcaag
     5341 gtgccgggtt ttacctgaac gcaacgcaag cgccgtggaa tgaacattac aaaatgtatg
     5401 actatatccg caacgagctg ccggatttag tgatgcaaca ttttccggca acggccagaa
     5461 agtctatctc aggtcattct atgggcgggc tgggtgcact ggtgctggcg ttacgtaatc
     5521 cagatgaata tgtcagcgtc tcggcgtttt cgcccattgt ctccccatcg caagtaccgt
     5581 ggggacagca agcctttgcc gcatatcttg gtgaaaataa agaggcctgg ttggattacg
     5641 atccggtgag ccttatttca caaggtcaac gtgttgcgga aatcatggtt gatcaggggt
     5701 tgagtgatga tttttacgca gaacagctgc ggactccaaa tcttgaaaag atctgccagg
     5761 agatgaatat caaaacgtta atccgttatc acgagggtta tgatcacagc tattattttg
     5821 tctccagttt tattggcgag catattgcct accacgccaa taaactgaat atgcgttaat
     5881 aatagtgcac gactgccgga tgcggcgtga acgccttatc cggcctccgg atggcgcggg
     5941 aatttgtagg catgataaga cgcggcagcg tcgcatcagg catctgcgca cgacttatct
     6001 atgtttacca catcaaatca tcaggcactt tgaagtcggc atacggatcg tcttcatcct
     6061 gctcttcggc gctgagcgcg ctgtgcaaca caatactgct ggcatcacgc tgagcaattt
     6121 tatcggcaac gctggcggga ataatggcgt attcaccttc gctattgtta tcgaccaaca
     6181 agcgggcaat cgccagacga ccattaatca actgcgcttg cgtgagctta tcgacaaaga
     6241 ttttcttaat cagattaccg tcagtgaagt taaaaccaat atcgccattg gcaatggtga
     6301 ttcggttcat ttcgataagc tgcttcacct gagctttata ttctttcgcc aacgccgctt
     6361 gtttttgctg ctcgctaagc tgtttatcac gctcaagctg tgcctttttg ttttcttcta
     6421 ccgccgcccg agcttcacgc gcctgaacac gcgatttttt cgccgttctc tcgaccttcg
     6481 ccgctttttt gctggtgact aatcctgctt tgagcaattg ctcttgtaag gtaagttttg
     6541 ccatctttgt ttccaaaccc gctgaataat ttttagcgag tatacctgta aacgccgtag
     6601 gtgtattggg ggcaagtccc tttcgcccct acttaataag gcgcgtcagg catcggcgca
     6661 cggctgcatt cgtgatctcc ggctcatcaa aataaacact gccaggccag caacaagaat
     6721 acccggcgca gaagcggcca ttacgccaac cgtgccagtg cctaatgcca gcattttccc
     6781 ggccagtaac ggaccgctca tagcccccag acgccctacc gccacggctg ttcccacacc
     6841 tgttgcgcgg atctgcgaac tgtaaaacaa cggtgccagg gcatacaaaa cgctttgccc
     6901 acctgtcgca aacaaccccg cgacgaatcc cgccagcagc ataccgttaa atgacgacac
     6961 cgttcccagc gccagcagcg aagctaacat gccgctataa atcagtagcg acatggttac
     7021 tggacgcagc ttatccatca atgcgcccaa cattaacgtc ccgcttgccg cccccatttg
     7081 cagtgcaaac atcacccctg ccgcctgcga tggctggaat ccttgctcca ccaaaagcag
     7141 cggtagccag ttgatcaaca tgtagaccac cagcagagtg aagaaataac acaaccacag
     7201 cagcagcgtc gcggttgccg tttctggcgc aaataacgca cgcagtggtg gcgcagcctg
     7261 tttttcgcca gcgaaaaccg ccgactccgg cagccagcgc attaatagtg gcaccagaat
     7321 caacggcact acaccaccga cccaaaacac cgtttgccag gctaagtttg cccccgcgaa
     7381 acccagtgtc gccgccagcg ccgcgccaat ggggacaccg caatacatca ggctcactgc
     7441 cgtcccacga aaacgtggac ccgcggcttc agacgtcagg gcgatcagat tcggcaacgc
     7501 cgcccccagc ccgacaccgg tcatcagtcg cgcaaagacc agtgagggga aatcccaggc
     7561 aatcgccgtt gccagtgaga acaaaccaaa cagcgcaact gagctaatta aaatacgctt
     7621 acgaccataa cggtccgcca gcattccgcc aaccagcgcg ccgggtagca aaccgagtat
     7681 tccggcgctg aatatccagc ccatttgcat tttatcgagt gcgaaagcct gggcgatgcc
     7741 acccgccgca atgccagccg cctgaagatc cagtccttcc atcagagcga ccaaaaaaca
     7801 aagcccgata gtcagcatca ggcgggttga agatgatgaa ggggtacgag tcgacataag
     7861 atgaacctcg ttgcgagtgc aggtgaatcg cggctgatac attgtcagcc gcgtaatcag
     7921 aaccatttaa tgactgccta cgggcgaaat ataggccgga taaggcgttt acgccgcatc
     7981 cggcagtcgt gctgggatgg gcatacaaaa tgcacgttct cggcagtcat cattatttgt
     8041 tgttgcgcag atccagcgcc acgtcgacga tcatatcctc ctggccgcca accatccggc
     8101 gcttgcccag ctcaacgaga atatccaccg cgcttaagcc ataacgcgcc gccgccgttt
     8161 cacagtgacg caggaagctg gagtaaacac cggcatatcc cagcgccagc gtttcgcgat
     8221 cgactcgtac cggacgatcc tgcaacggac gcaccaggtc gtcagcggca tccattaacg
     8281 catagagatc ggtcccatgc tgccaaccca gtttatccgc ggcggcaata aacacttcca
     8341 gcggcgcgtt acctgcgccc gcccccattc ccgcgagact ggcatcgatt cggtcgcagc
     8401 cctcttccac cgccgcgata gagttcgcca cgccaagact caggttatgg tgagcgtgca
     8461 tgccggtttg cgtttctggt ttcagcactg ctttcagggc gcggaaacgg tcacggatat
     8521 cgctcatgtt catcgcaccg ccagaatcca ccacataaat acaggtcgca ccgtagcctt
     8581 ccatcagctt tgcctgcttt gcgagattct ccggcgtggt catatggctc atcatcagaa
     8641 aaccaacggt gtccattccg agctcgcggg catactgaat atgctgggcg gaaacatcgg
     8701 cttcggtaca gtgcgttgcc acacgaacca cccgcgcgcc agcctgccag gcatttttca
     8761 aatcgtgaat agtgccgatt ccaggcagca acaacgtcgc gattttggcg tgcttcacca
     8821 catccgccgc cgcttcaatc cattcaaggt cgctatgtgt gccgaaacca tagttaaagc
     8881 tggacccctg caaaccgtcg ccatgggcca cttcaatcga atccacgcgg gcatcgtcca
     8941 gtgctttggc aatctggcgg acattttcca gcgaatactg atgacgaatg gcgtgcatac
     9001 cgtcacgcaa cgtgacgtcc gagatataaa gttttttacc gttcatgccg cttctcctgc
     9061 cttgcgcgcc agtgactggg ccattttttc cgctgtcgcc agcgcactgg aagtcataat
     9121 gtcgaggttg cccgcatagg caggcagata atgcgctgcg ccttcgactt ccagccagac
     9181 cgccgttttc agcccggaga actgccccac gcctggtaaa ttgaccggtt tatcctgcgg
     9241 gatgacttca aactgcacgc gctgtttcag gcgataaccc ggtacgtaag cctgcaccgc
     9301 ctcagccatt tcattgattg aggcttcgat atcatcttgt gaagcttcat cgctcaatac
     9361 atacaccgtg tcacgcatca tcaacggtgg ctctgctggg ttaagcacaa taatcgcctt
     9421 cccttttgcc gcgccaccca ccacttcaat ggcgcgcgaa gtggtttccg taaattcatc
     9481 gatattggca cgcgtgccag gtccggcaga tttactggca atagaagcga taatttcggc
     9541 gtattgaaca cgcgccacgc gtgaaactgc cgccaccatt ggaatggtgg cctggccgcc
     9601 gcaggtgacc atgttgacgt tcagttgatc gacattcgcc tcgagattaa cgaccggcac
     9661 gcagtagggg ccgatggcag caggcgtcag gtcaattaag cgaatatccg gtttcgcttc
     9721 gcgcaaagcg gcatcgtttt tcacatgcgc acccgcgctg gtcgcatcaa atacaatgtc
     9781 gatatcagca aattcaggca tgttcatcag cccgatcacc ccttcatggg tggtggcgac
     9841 gcccatacgt ctggcacgcg ccagaccgtc ggactgagga tcaatgccga ccatcaccgc
     9901 catctccaga tgctgaccgt gacgcaaaat tttaatcatc agatcggtgc caatgttgcc
     9961 agaaccgata atggcgactt tacgcttact catgacagac ttccttttgg ggctgcgctt
    10021 gaaaatgtcg cagcaactga acctatgcct tcgatatggg cttcaaaacg atcgcccgca
    10081 ttcaccgcca ccatcggacc taatgccccg gtaagaatga tatctccggc gcgcagcggt
    10141 tcacccaggc tggccatttt gcgtgccagc cagacggcgg cattaagcgg atgtcccagg
    10201 cattcgctgc cgcgcccgct ggaaacctct tcgttattac gcgtcatctt catggcgcag
    10261 tttttcaggt ctaaccccgc cggacgttgc gccggaccgc cgatgacata cactccacag
    10321 gaggcgttat ctgccacggt atcgacaaac tgaatcgacc agtcgcgaat gcggctcccc
    10381 accacttcca gcgccggaag tacccattca atggcgttat acaattcgtc gaaggtgata
    10441 tcggttgcgg gtagatcgcg gttcaacacc agtgcaatct ccgcttcaat gcggggttgc
    10501 agaacacggg aaaaaggaat ggtttcgtta tcgccataac acatgtcggc aaataatgtc
    10561 ccgaaatccg gttgatcaac gcccagttgt tgctgcactt tcggatgcgt cagccccact
    10621 ttgcgcccca ccactcgccg tccctgagcg acgtcatgtt gcacattgat gtgctgaatg
    10681 gcgtaggccg cttcagcgtt atcgatacca atcagatcgc gcagcggcgc aatcgcttcg
    10741 ccctgctctg cggcgcggcg taaatccgcc gccagttgct caagagtatg cttcgtcatg
    10801 accgttcctt aagggcgtgc gaggaaattc agcaccagtt gattgaaagc gtcggcatgt
    10861 tcccactgcg cccagtgacc acagtcgcgg aagatatgca gttcagaacc ggcaatgccg
    10921 gacagcagac gcagacccgc atccatcggc acaaagcggt cgttgcgccc ccagacaatc
    10981 agggtttgcg ctttgatttc cgccagacgt gggccaaaat ccgggaactg tttcggattg
    11041 gcttccaggc tcttaacgaa gttttccagg tgatcgcggc gcgacagcat attattcagg
    11101 cgcgcttcaa acagggcgtc ggtcaaatcg ctggtatcaa aaacgaagat atccatcatc
    11161 agcttcaggt tttcgatagt cggctgacga taaagctgat tcagtcgctt aataccttcg
    11221 gttggcatcg gcgtaaacaa actcatgccg cccgtaccgc cgcccatcag caccagtttg
    11281 ccgacgcgct ccggccagtt aagagtgaac gccacagaac tgtggccgcc catcgagttg
    11341 cccagcaggt ggattttggc gatatccagt tgatccacca cgcttttcag gattcgcgca
    11401 ttaagatccg atcgcgaacc actattaacg atcgaatcgc tcttacccca acccggacaa
    11461 tccagcagga tcacccgata gcccgcctct accagcggat cgatattgcg gctgaagttc
    11521 gcccagccag tagcacccgg gccggaacca tgcagcagga caacagtttc gtcgccttgt
    11581 ccgcagtcat taaaatggat gcgcagcgtt ttacccgctt cttctacatt cagaaaacgg
    11641 ctggtggcgg cttcggtttg tggctgataa ctcatcatct tctcctgcat attcagttct
    11701 ctgttctggc gcttaacgag ccaaatccgg caatccactc cgggattggg cggtaataac
    11761 gcccttcgct acgccagttg ccaaacgcag aaatagcggc aaaagcggcg acccaggttt
    11821 tgatttcatg tgtcgacttt ccggcaatgg cggaaagctc ttcgttactg acggcatcca
    11881 gttcctgtat gcgtccctgc tccagcaaag tcatgaactg gttatcccaa atcgggttga
    11941 gcggatgcag agttctctga tcctcaacaa acttctcagc ggcgctaatc acccgctgct
    12001 gacgcaattc acgctcagtg gcgggtaaat ctttcccgct ccccaacaga cggtcgcgca
    12061 tatgggcatc ggctttcgcc agttctggca ccggcggctg atgggaaagc ccaccggacc
    12121 caaggaacag cacgcgttta ttgagagtgc tggtgaaacg tccaatggct tcccccaaca
    12181 tgcgggtacg ctggaaaccg ggcagcggtg tggcgacacc gttgatgaac acaggcagaa
    12241 ccggcacctt atccagccca ccgagcagga actccagcgg ctgggcgaac ccgtggtcca
    12301 cctgcataca gtaagaaacg gcaagatcga tcccgctctt catgacggca tgcgcacagg
    12361 cctccgccag ctccacaggc acgggcagct ctcctgccgc actgccgaaa tcaccaattg
    12421 ccgtcgctcc aacgcctaaa cagaacggtg gcatcacgtc atagaaaaag ccgttgtagt
    12481 gatctggcgc aaacagcacc accagttcag gggagaaagc cgcaatacgc tcgcgggcgc
    12541 tggcaatcac gccattgacc tcatcgagca cctcttgcgc cgggtcgaca taccccacca
    12601 gcggcgagtg ggaaagacag tgaagataag cgtacatatc aggctacctt ttcgacagaa
    12661 acgtcggcat cagggcgggt cagcgtcatc accgacgcca gtttattcag ggtcttgccc
    12721 agggtttgcg gaatggcggt ggcggcaaca aagcgatccg ggcgcatcac caccagcgaa
    12781 gcattgtgtt gcgcgaacca gctacgcagg cgaccttgcg tatcgcccac gcgtagtacg
    12841 ccgtcgtggt tatcctgtgc ggtatgaatt tgcacttccg gcaccacctg aatgaagcgt
    12901 gtgcccaacg cgcgccactg ctggatttgc tcgtcgctca tcccccacag tggattgcat
    12961 ccccagccaa ttaccgcgaa gttcgcgccg atcgcgttat cgagcagcgt cacgtcgccg
    13021 ttttccagcg tgactttcgg ctgaataaac atcttgccga ccggagagtg cttcgcttcg
    13081 ccctcacgca ccagcgcacc gccgtaatat tgcggcatcg gcttgaagcg catttcgagg
    13141 aagtagcgtt ttactggcgg cagataattc aacagccagg aaacgccgtc acgtaacgta
    13201 ccctgccagc gtttcggtgg agccagcacg ttgcccgccg tcacggacag gtcaatcatc
    13261 gctttggcgt gatcgcgacg ttcttgttga taggtatcga gcagcgcatc gcgggctttc
    13321 ccctggataa ccaacgccag tttccatgcg aggttaaagg cgtcgcgcat accactgtta
    13381 tagccctgcc cctgccacac cggcatgatg tgcgcggcat cgcccgccag cagtacgcga
    13441 tcaatacgaa aacgttgcgc cagtcgcgcg ttgtgggtgt agacacgctg gcgaatcaat
    13501 tcaacattgt ccggattagg cagcactttg cttaacagct tgcgcatatt ttgcggctca
    13561 cgcagctgtt cttcggtttc tcccggcatc accataaatt caaagcgacg taccgcatga
    13621 ggcagcgcgg cagaaacata cgggcgcacc ggatcacaac acaaatagat atgcggcgta
    13681 cttaacggat cgttggcgat atctaccaca atccactgat ttggcgcagt tttaccttca
    13741 aacggcacat tcagggtgcg acggacaaaa cttgccccac catcacaggc caccagccac
    13801 tgggctttga ccgtttcccg ctgcccttct gccgttttca ggtgcaaggt cacttcgtca
    13861 tcttgctggc tgaaggcctc cagctcgcgg gaaaacaggc agcgcacatt cggaaaacgc
    13921 gacaatcctt ccagcatcac cgcatcgacc tgaggctgaa taaaggcgtt acggcgcggc
    13981 cagccaaatt catcggtcat tggctgaata tcagcaaaac agcgaccttt tggggtgaga
    14041 aaacgcatcg cgtgccacgg cgtagtgtgc ggcagaacat tatcgaccag gccaaccgac
    14101 tgcatggtgc gcagcgcctc gtcatcaata ccaatcgcac gcgggtagtc gatcaactta
    14161 tcgagtttct ccaccaccag cacgtcaatg cccatctggc cgagatagtt cgccatcatc
    14221 agcccgaccg gaccggcacc agcgatcgcc acctgaacgc tatggttaac agcaggctgg
    14281 atgtcagggt gttgtattgc catttcagta cctcacgact cggacaaaat gccgttgcgc
    14341 gcacagtaca gcgcaactta ttttgttaaa aacatgtaaa tgatttttta ttgtgcgctc
    14401 agtataggaa gggtgttttc ggctacaatc aaaacatgcc cgaatgtgca ccaggtgcac
    14461 cacgttgttt taaatatata aatgtcaatt aatatgcaga acaatgagca gacggaatac
    14521 aaaaccgtgc gcggcttaac ccgcggtcta atgttattaa atatgttaaa taaacttgat
    14581 ggcggtgcca gcgtcgggct gctggcggaa ctcagcggcc tgcatcgcac cactgtgcgg
    14641 cgactgctgg agacgctgca ggaagaggga tatgtccggc gtagtccctc cgatgatagt
    14701 tttcgtttga ccatcaaagt gcggcaatta agcgaaggat ttcgtgacga acagtggatt
    14761 tctgcactgg cggccccgct gctgggcgat ctgttgcgcg aagtggtttg gccaacagat
    14821 gtgtccaccc tggatgttga tgcaatggtg gtacgcgaaa ctacccaccg tttcagccgc
    14881 ttatcctttc accgggcaat ggtcgggcga cggttaccgc ttctgaaaac cgcctcgggc
    14941 ctgacctggc tggccttttg cccggaacaa gaacgcaagg aattaatcga aatgttagcc
    15001 tcccgccccg gtgatgacta tcaactggca cgggaaccgt taaagctgga agccattctg
    15061 gcgcgcgcgc gcaaagaggg ttacggacag aactaccgcg gctgggatca ggaggagaag
    15121 atcgcctcta ttgccgtacc gctgcacagt gaacaacggg tgattggctg tctgaatctg
    15181 gtgtatatgg cgagcgcaat gaccattgaa caggcagcgg aaaagcatct tccggcgctg
    15241 caacgggtgg caaaacagat cgaagaaggg gttgaatcgc aggctattct ggtggccgga
    15301 cggcgaagcg gcgtgcattt acgttgacac catcgaatgg cgcaaaacct ttcgtggtat
    15361 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgct
    15421 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
    15481 ggccagccac gtttctgcga aaacgcggga aaaagtagaa gcggcgatgg cggagctgaa
    15541 ttacattccc aaccgcgtgg cacaacaact ggcgggcaag cagtcgttgc tgattggcgt
    15601 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
    15661 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
    15721 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
    15781 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcttt
    15841 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
    15901 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
    15961 aggtccatta agttctgtct cggcacgtct gcgcctggcg ggctggcata aatatctcac
    16021 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagcgcca tgtccggttt
    16081 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
    16141 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
    16201 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcgtgttata tcccgccgtt
    16261 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
    16321 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
    16381 aaaaaccacc cttccgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    16441 aatgcagctg gcacgacaag tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    16501 atgtgagtca gctcactcat taggcacccc aggctttaca ctttatgcgt ccggctcgta
    16561 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg atacagctat gactatgatt
    16621 acggattcac tggccgtcgt attacaacgt cgtgactggg aaaaccctgg cgttacccaa
    16681 cttaatcgcc ttgcggcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    16741 accgatcgcc cttcccaaca gttgcgcagc ctaaatggcg aatggcgctt tgcctggttt
    16801 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact
    16861 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcctatcta caccaacgtg
    16921 acctatccca ttacggtcaa tccgcctttt gttcccgcgg agaatccgac aggttgttac
    16981 tcgctcacat ttaatattga tgaatgctgg ctacaggaag gccagacgcg aattattttt
    17041 gatggtgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag
    17101 gacagccgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg aaaaaaccgc
    17161 ctcgcggtga tggtgctgcg ctggagtgac ggcagttatg tggaagatca ggatatgtgg
    17221 cggatgagcg gcattttccg tgacgtctcg ttgctgcaca aaccgaccac acaaatcagc
    17281 gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgcggtact ggaggcagaa
    17341 gttcagatgt gcggcgaact gcgcgatgag ctgcgggtga cggtttcttt gtggcagggt
    17401 gaaacgcagg tcgccagcgg caccacgcct ttcggcggtg aaattatcga tgagcgtggt
    17461 ggttatgccg atcgcgtcac gctacgtctg aacgtcgaaa acccggcgct gtggagcgcc
    17521 gaaatcccga atctctatcg tgcagtggtt gaactgcaca ccgccgacgg cacgctgatt
    17581 gaagcagaag cctgcgatgt cggtttccgc gaagtgcgga ttgaaaatgg tctcctgctg
    17641 ctgaacggca agccggtgct gattcgcggc gttaaccgtc acgagcatca tcctctgcat
    17701 ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac
    17761 tttaacgccg tgcgctgttc gcattatccg aatcatccgc tgtggtacac gttgtgcgac
    17821 cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg
    17881 aatcgtctga ccgatgatcc gcgctggcta ccagcgatga gcgaacgcgt aacgcgaatg
    17941 gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc
    18001 cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg
    18061 gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac
    18121 gcgcgcgtgg atgaagacca gcccttcccg gcggtgccga aatggtccat caaaaaatgg
    18181 ctttcgctgc ctggagagac gcgcccgctg atcctttgcg aatatgccca cgcgatgggt
    18241 aacagtcttg gcggcttcgc taaatactgg caggcgtttc gtcagtaccc ccgtttacag
    18301 ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac
    18361 ccgtggtcgg cttacggcgg tgattttggc gatacgccaa acgatcgcca gttctgcatg
    18421 aacggtctgg tctttgccga ccgcacgccg catccggcgc tgacggaagc aaaacaccag
    18481 cagcagtttt tccagttccg tttatccggg cgaaccatcg aagtgaccag cgaatacctg
    18541 ttccgtcata gcgataacga gctcctgcac tggtcggtgg cgttggatgg caagccgctg
    18601 gcaagcggtg aagtgcctct ggatgttgct ccacaagata aacagttgat tgaactgcct
    18661 gaactaccgc agccggaaag caccggacaa ctctggctta cggtacacgt agtgcaaccg
    18721 aacgcgaccg catggtcaga agccggacac attagcgcct ggcagcagtg gcgtctggcg
    18781 gaaaacctca gcgtgacact cccctccgcg ccccacgcca tcccgcatct gaccaccagc
    18841 gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc
    18901 tttctttcac agatgtggat tggcgatgaa aaacaactgc tgacgccgct gcgcgatcag
    18961 ttcacccgcg caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct
    19021 aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc ggcgttgttg
    19081 cagtgcacgg cagatatact tgccgacgcg gtgctgatta cgaccgctca cgcgtggcag
    19141 catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtgttcaa
    19201 atggcgatta ccgttgatgt tgaagtagcg agcgatacac cgcatccggc acggattggc
    19261 ctgacctgcc agctggcgca ggtcgcagag cgggtaaact ggctcggatt agggccgcaa
    19321 gaaaactatc ccgaccgcct tactgcggcc tgttttgacc gctgggatct gccattgtca
    19381 gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa
    19441 ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaatatcag tcgctacagc
    19501 caacaacaac tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg
    19561 ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagtg
    19621 tcggcggaat tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa
    19681 taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac
    19741 tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta cttttttatc
    19801 atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca tatcagcaaa
    19861 agtgatacgg gtattatttt tgctgctatt tctctgttct cgctattatt ccaaccgctg
    19921 tttggtctgc tttctgacaa actcgggctg cgcaaatacc tgctgtggat tattaccggc
    19981 atgttagtga tgtttgcgcc gttctttatt tttatcttcg ggccactgtt acaatacaac
    20041 attttagtag gatcgattgt tggtggtatt tatcttggct tttgttttaa cgccggtgcg
    20101 ccagcagtag aggcatttat cgagaaagtc agccgtcgca gtaatttcga atttggtcgc
    20161 gcgcggatgt ttggctgtgt tggctgggcg ctgtgtgcct cgattgtcgg catcatgttc
    20221 accatcaata atcagtttgt tttctggctg ggttctggct gtgcactcat cctcgccgtt
    20281 ttgctctttt tcgccaaaac ggatgcaccc tcttccgcca cggttgccaa tgcggtaggt
    20341 gccaaccatt cggcatttag ccttaagctg gcgctggaac tgttcagaca gccaaaactg
    20401 tggtttttgt cactgtatgt tattggcgtt tcctgcacct acgatgtttt tgaccaacag
    20461 tttgctaatt tctttacttc gttctttgcc accggtgaac agggtacgcg ggtatttggc
    20521 tacgtaacga caatgggcga attacttaac gcctcgatta tgttcttcgc gccgctgatc
    20581 attaatcgca tcggtgggaa aaacgccctg ctgctggctg gcactattat gtctgtacgt
    20641 attattggct catcgttcgc cacctcagcg ctggaagtgg ttattctgaa aacgctgcat
    20701 atgtttgaag taccgttcct gctggtgggc tgctttaaat atattaccag ccagtttgaa
    20761 gtgcgttttt cagcgacgat ttatctggtc tgtttctgct tctttaagca actggcgatg
    20821 atttttatgt ctgtactggc gggcaatatg tatgaaagca tcggtttcca gggcgcttat
    20881 ctggtgctgg gtctggtggc gttgggcttc accttaattt ccgtgttcac gcttagcggc
    20941 cccggtccgc tttctctact gcgtcgtcag gtgaatgaag tcgcttaagc aatcaatgtc
    21001 ggatgcggcg cgagcgcctt atccgaccaa catatcataa cggaatgatc gcaatgaaca
    21061 tgccaatgac cgaaagaata aaagcaggca agctatttac cgatatgtgc gaaggcttac
    21121 cggaaaaaag acttcgtggg aaaacgttaa tgtatgagtt taatcactcg catccatcag
    21181 aagttgaaaa aagagaaagc ctgattaaag aaatgtttgc cacggtaggg gaaaacgcct
    21241 gggtagaacc tcccgtctat ttctcttacg gttccaacat ccatataggc cgcaattttt
    21301 atgcaaattt taatttaacc attgtcgatg actacacggt aacaatcggt gataacgtac
    21361 tgattgcacc caacgttact ctttccgtta cgggacaccc ggtacaccat gaattaagaa
    21421 aaaacggtga gatgtactct tttccgataa cgattggcaa taacgtctgg atcggaagtc
    21481 atgtggtcat taatccaggc gtcaccatcg gggataattc tgttattggc gcgggtagtg
    21541 tcgtcataaa agacattcca ccaaacgtcg tggctgctgg cgttccttgc cgggttattc
    21601 gcgaaataaa cgaccgggat aagcactatt attacaaaga ttataaagtt gaaccttcag
    21661 tttaaattag cactgaaata aatgtcggat gcgccattgg gcacatccga caataattca
    21721 ctcttccatt atctggcgaa acaagcgtcg catcatgcct ctttgaccca cagctgcgga
    21781 aaacgtgctg gtgcaaaacg cagggttatg atcatcagcc caacgacgca cagcgcatga
    21841 aacgcccagt ccatcaagta attgccactg atactacgca gcacaccaga aaaccacggg
    21901 gcaagcccgg cgatgataaa accgatcccc tgcataaacg ccaccagttt gccagcaata
    21961 gcgggttgcg cagagtgatc gagcgccagc agcaaacaga gcggaaatgc gccgcccaga
    22021 cctaacccac acaccattgc ccacaatacc ggcaattgca gcggcagcca gataaagccg
    22081 cagaacccca ccagttgtaa cgccagcgcc agcatcaaaa gtttgcgccg atcctgatgg
    22141 cgagccatag caggcatcag caatgcccct gctgcttgcc caagcgtcat caatgccagc
    22201 aaggaaccgc tgtactgcgc gctggcacca atctcaatat agaaagcggg taaccaggca
    22261 atcaggctgg cgtaaccgcc gttaatcaga ccgaagtaaa cacccagcgt ccacgcgcgg
    22321 ggagtaaata ccacgcgaac cggagtggtt gttgtcttgt gagaagaggc gacctcgcgg
    22381 gcgctttgcc accaccaggc aaagagcgca acaacggcag gcagcgccca ccaggcgagt
    22441 gtttgatacc aggtttcgct atgttgaact aaccatggcg tcatggcggc accaagtcca
    22501 ccgccgccca tcagagccgc ggaccacagc cccatcacca gtggcgtgcg ctgctgaaac
    22561 cgccgtttaa tcaccgaagg catcactgcc tgaatgatgc cgatccccac tccaccaagc
    22621 agtgcgctgc taagcagcag cgcactttgg gggtaaagct cacgcatcaa tgcaccgacg
    22681 gcaatcagta acaaactgat ggcgacgctg cgacgttcgc tgacatgctg atgaagccag
    22741 cttccggcca gcgccagccc gcccatggta accaccggca gagcggtcaa cagggcagcc
    22801 acgctaaagc tcattccgct tgcctggcgc aattgcggta gcagtggccc gacggaggtg
    22861 agcagtggtc gcatattaag accaatcagc accagaacca gcagcatcgt gaacgtcttt
    22921 tgatggttgc gcatggctgt cagaacggtt tggtcggcag atatttacca tctaaggtga
    22981 tgaccgcacg ttcgccacct tccgggtccg ccactttctt aacgtcgagt ttgaagttaa
    23041 tcgcgctgat aatgccatcg ccaaacttct catgaaccaa cgctttcagg gttgtgccgt
    23101 acacctgcaa catttcatag aaacgataca tcgttgggtc ggttggaata cgttcatcaa
    23161 tgcagccacg cagtggaatc atctgcaaca gtagaatggc gtcttcgtcg agatccagct
    23221 tcgctccgac caggcgggcg gcgtcggcag gaagcgcctg ctgccccagc aaagccgcgg
    23281 ttacaaaggc ttctgccaga ccggtgccgt cggcaatctc ggcaaatgag agatcttttt
    23341 tagctttgct gagcaaaatg gcatcggcaa gatcaagacg aatattgcgg ttaatttgcg
    23401 actgaatcat ggtggatctc ctgatggttt aaaaataagg ttacgctgcg gtcggttggc
    23461 gtagcggtat ggcacaaacg cgaggattag cggccagtgg cacaaactgg cgggttgcgc
    23521 cgtcaaaagc tgcgatgctg ccgctttcaa tgtcgtagac ccagccgtgc agggcgatcc
    23581 gcccctcttc gagcgccagg cgcaccgatg gatgagtttg caaattagcc aactgagcaa
    23641 tgacgttttc acgtaccatc gccgcagctt ttgacggtaa atcgggatgc gggcgcgcct
    23701 cattaacgac gcgggctgaa tcggcataac gcagccagtg agagacggca ggcatatggt
    23761 ccatgcactg gcagctggca atagcggtca ttgcgccaca gttggaatga ccgcaaatca
    23821 caatgtcaga tacccgaagc gcagcgacgg cgtactccac cgaagcagaa acgccaccgg
    23881 gttccggccc gtaggaaggg acgatattgc ccgcgttgcg aataacgaac agatcgccag
    23941 gctcacgttg cgtcaccagc tcagggacca gacggctgtc ggagcaggag ataaaaagtg
    24001 tgcgcgggct ttgctgtgtc gccagctgtt taaacaaggc ttcccgcttc ggaaatgcct
    24061 cgcgctggaa tttaaggaat ccatcaataa tctctttcac cgttaacctc tgtctgtctc
    24121 tggaatgaga ggcagactac tcgccgattg tcataaggta aaagtctcat ttatgatgag
    24181 ttctattgga tttacttata ggttggtgaa tgctctctcg acatatcaat tattttcttg
    24241 ccgtggctga acatggcagc tttacccgtg ccgccagtgc gttgcacgtc tcccagcctg
    24301 cgctttccca gcagattcgc cagttagagg agagtttagg cgtgccgctg tttgaccgta
    24361 gcgggcgaac gattcgtctc actgatgcag gagaagtctg gcgacagtac gccagccggg
    24421 cgttacagga actgggggcg ggtaaacggg cgattcatga tgttgccgat ctgacgcgag
    24481 gatcgctgcg tatcgccgtc actcccacct ttacaagcta ctttatcggc cctttaatgg
    24541 cggatttcta tgcgcgctac cccggcatca cgctccagct acaggaaatg tcgcaggaga
    24601 aaatcgaaga tctgctttgt cgcgacgagt tggacgttgg gattgccttc gcgcctgtgc
    24661 attcgccgga gctggaggca attcctttac tgacagaaag tttagcgtta gtcgtggcgc
    24721 aacatcatcc gctggctgcc tgtgaacagg tggcgttgag tcgcttgcat gatgaaaaac
    24781 tggtcctgct cagcgcggaa tttgccacca gagagcaaat tgaccactac tgcgagaaag
    24841 cggggctaca tccacaggtg gtcattgagg cgaactcaat tagcgcggtt ctggagctga
    24901 ttcgccgcac ttccctttcc acattgttgc cagcagcgat tgccacacaa catgacgggc
    24961 ttaaagctat atcccttgcc ccaccgttac tggagagaac ggcggtttta ttgcggcgga
    25021 aaaatagctg gcagacagcc gccgcgaagg catttttgca catggcgttg gaggaatgcg
    25081 cggttgttgg cgggaatgaa tcacggtagc cactgacaca ggtcagtggc tcttcaacac
    25141 agaggaaatt accgtttgta atcgatggct tctggctgat ccagatatac ggtggtttgt
    25201 gccggttgtg tgctggcaat caccttaccg ccacgtaccg aataacgtac aggaacctga
    25261 cggcgcagcg catcaaaccc attttctgca ggcaggataa tcaggttggc gctgtttccg
    25321 gcggcaatgc cgtaatcctg caaattcaac gtcctggcgc tatggtgggt gattaaattc
    25381 aggccgtcgt taatctgccc gtagcccatc aactgacaaa catgcagccc catatgcagc
    25441 acttgcagca tattcgccgt tcccagtgga taccacggat cgaagacatc atcgtgacca
    25501 aagcagacgt taatgccgga ctccagcatc tctttaacgc gcgtgatgcc gcgacgtttt
    25561 ggatacgtat cgaaacgtcc ttgcagatga atattgacca gcgggttggc gacaaagtta
    25621 ataccggaca ttttcagcaa gcggaacagg cgcgaggtat acgccccgtt ataggagtgc
    25681 attgccgtag tgtggctggc ggtgactcgc gcgcccatgc cttcacggtg cgccagggca
    25741 gcaacggttt caacaaagcg cgactgttcg tcatcaatct catcacagtg aacatcgatg
    25801 agacggtcgt atttttgcgc cagggcgaag gttttatgca gcgactccac gccatattca
    25861 cgggtaaatt caaaatgcgg aatcgccccc actacgtctg cccctaagcg taacgcctct
    25921 tccagcaacg cttcaccgtt gggatacgac aaaatccctt cctgcgggaa ggcgacgatt
    25981 tgcagatcaa tccacggcgc gacttcctgc ttcacttcca gcattgcttt cagcgcggtc
    26041 agcgttgcat ccgaaacatc gacatgggta cgcacatgct gaatgccgtt ggcaatctgc
    26101 catttcagcg tttgccaggc gcgttgtttc acatcgtcat gggttaataa cgctttgcgc
    26161 tcggcccagc gttcaatgcc ttcaaacagc gtgccagact gattccagtt cggttgtccg
    26221 gcggtttgcg tggtgtccag gtgaatatgt ggctccacaa acggcggtat aactaaacct
    26281 tgttcggcat ccaggctgtt ttcagttacg ggcatcaccc cggattgcgc atcaatggcg
    26341 ctgatttttc cgtccagcag atgaatctgc cacagcccct ctttgccggg taaccgggcg
    26401 ttaataattg tctgtaaagc gttattcgac actgttagcc tccacatgcg tcattgcagc
    26461 cgtcgttttg cgattcaaaa tcgggttaag gatcagatag ctcagcgcgc cacctaatac
    26521 cgcgttgacc ggaacaatac ccggtaacca gtggcctgcg gcaatcccca aggcgaccgc
    26581 cagaatcgcc acccaattga cactcatcat acgcgtggtc gcaaagtgct catagcggcg
    26641 acggttcatc agatagtcag cgatgatcac gccccctacc ggaggaatag ctgccgaaag
    26701 gaaggtcagc cagccgacaa aattgttata cagccataat gcgcagaccg taccgataat
    26761 accgttgatt accgaaaggg ttttgctcga cataccagta atgttggcga aacctaagcc
    26821 cgacgcatag agcgcgttat cgttggtggt ccagatattc agccccagca ccacaatcgc
    26881 aggcagcagc agaccctgag caatcatcac atcagagata tccgccatcc ccagtgccgc
    26941 cgcgcccgct gcaccgaaaa taaacatcaa cgagttgccg aggaaaaagg ccaccatcgc
    27001 caccagcacc gccagtttgg cattacgacc aaaccggaca aagtcagcgg tgagcgtgcc
    27061 cgcactgata aatgacccca caaccagcgc cagcgcgaca ttgaaatcta acggttgtgc
    27121 gggaatgacc gcttttaatg cgtccaggcc gcccatgccg ttaacggcca gccacacgga
    27181 ataaccgcca aggcaggcga tagccggaac cgcaatcacc gaaagaaccg tcagcgccga
    27241 aatgccaaag aagacggtga cggtcatcag taaaccggaa atggcaatca gcaaattaat
    27301 atccagcccg gttgccttgc ccaccggaat ggcaaacatc gccacgccaa cgccaaacca
    27361 gccaacctgg gtgccgccca gcagcagtga aggcagccat gagcctttaa caccaaaaga
    27421 gaagcgagca agaagatgag tggtcaggcc ggtttttgcg ccaatgtaac caagaaatga
    27481 agtgtaaata ccgaggagaa gattaccgat gaggactgcg aggaagaaat catgatagct
    27541 aagaccggtt ccgagagtgc cgccggtcca catactggcg gaaaagaagg ttaatcccag
    27601 catgacgaac gtcaatgcca ataccccttt ccgcgccgac tgcgggactg gcccctggct
    27661 aaagttgtta tcttgcgaca cgaaattccc cctttttgct gtctgaaacc caaaaaatcc
    27721 gccgcattct attcatctga agataaaaag caatcgtttt cgtagggaaa tatatttttt
    27781 atatgaatga gtgcaaaaag gtgggggaaa aattatcggt gtaaaaacag tgggtagcca
    27841 taataagacg cggtaagcgt cgcatcaggc atcggcgcat ggtgtcggat gcgccaggcg
    27901 acgcatccga caataaccta ctcttccatc gcctggcgga tctgatccaa cgacgcagga
    27961 tcatcaatgg tcgtcagatc cccaggatcg cgtccttcgc aaatcgcctg gatcgtgcgg
    28021 cgcagcattt ttccggatcg cgttttcggc aattgcgaga caaaccagac gtgcgccggg
    28081 cgaccaaagt tgccaatctg gctgtccacc agcgccataa tcgccttctc ttgcgagtgc
    28141 gccacgtctc ggtcttccag actgtcgctc tctttcggaa tgacaaacgc caccgccacc
    28201 tgccctttca gcgcatcttt caccccaacc actgccactt cggcaacgcc cggatgactg
    28261 gagatactct cttcaatctc gcgcgtcccc agccgatgcc cggcaacgtt aatcacatcg
    28321 tcagtgcgcc cgagaataaa gtgataaccg tcagcatcgc ggatacccca gtcaaaagtg
    28381 gcgtacaccg gacgggaaaa cagcgaccag tacgtcttca caaagcggtc gtcgtcgccc
    28441 caaatggtct gaatacaccc cggcggcaat ggcccttcca ccaccagcat ccctttctcg
    28501 ttgacgccac acggttcgcc ggtgacttca ttaagcaact gcacgttata gccatacatc
    28561 ggcacgccgg ggcttcccag acgcgtcggc ctgtcgtcca gaccgcgagc aatcgccata
    28621 atcggccagc cggattcggt ctgccagtag ttgtcgatga ccggcacatc cagcgtattg
    28681 ctcacccaac tggcggtcgg ctcgtccagc ggttctccag ccagatagag cacttccagc
    28741 gacgagagat cgtgtttgcg aatttcagcg gtagggaatt ttttcagcac gcgaatggcg
    28801 gtcggcgctg agaacatgcg gctaacctga tatttctcga cgattttcca ccacacgccg
    28861 cagtccggcc aggtcggtaa tccttcgtaa acgatagtcg ccatccccgc cagcagcggc
    28921 gcgtagacga tatacgaatg ccccaccacc cagccgatat ccgaggcaca gaagaacacg
    28981 ccgcccgctt tgccgccaaa aatggtgtcc atcgaggtcg ccagcgccac cgcatatccg
    29041 ccgacgtcac gctgcacgcc tttaggtttg ccggtcgtgc cggaggtgta gagaatgcag
    29101 gaggtttcgt tggattccag ccacgccacc ggtacccgcg cgccgatgtg ttgatggcgc
    29161 aacgacgcga aatcgacatc ccgcccgctg acgcgtgcca ttttcgccag cccgcgatcc
    29221 accagcaaaa catggcgtgg ctggtgctgc gcctgactta tcgcatcgtc gagcaatttt
    29281 ttatagggaa tgattttgcc accgcgcgct ccggcatcag ccgagacaat cagcaccggt
    29341 ttagcatcat caattcgcgc cgccacgctg tgcgaggcaa atcccccaaa caccaccgag
    29401 tgaatagcgc caatgcgcgc gcaggccagc agagtaatat gcgcttcggc aatcatcggc
    29461 atatacacca gcacccgatc gccacgctgc acgcccagtg agcgcagcat tgacgccacc
    29521 gcgttcactt cgtcatgcag ctgacgaaag gtaaaggtgc gctcttcttc tgtttccgaa
    29581 gagacggcaa tcagcgccag cgcctctggc tgtttctcca gccagcggtc gatagcgttg
    29641 tggcacaagt tggttcggcc ttcacaaaac caacgggcaa acggcgggtt gctgtggtcg
    29701 agcgtttgcg taaagggcgt ctgccagtca atacgccggg cctgctcggc ccagaactgc
    29761 tccggttcgt taatcgaacg ctgataaaat tcgctaaaag acataatgct ctcctgttga
    29821 acttacgcct taccgctgtt tgcttaaatg acgtacaggt cgagatactc attgaccggc
    29881 atctgttcca ggcgagctct gtcgagagaa acgtccagaa tgcgctgctg ttggcgagtc
    29941 gggaactggc gcgcgagatt gattttgaat ttatcgacca gcttcggaat gccatcctga
    30001 cggcggcgag catgaccaat tgggtactcc accaccactt cttcaaatcg cgtgccgtcg
    30061 gtgaactcaa gggttatggc attggcgatg gcacgttttt ccgggtcgtg gtagtcagcg
    30121 gtaaatgccg gatcttcaaa gcaattgatc ttctcgcgca gggcgtcgat gcgtttatct
    30181 tgcgccacgt tgtcctcgta atctgccgcc gttaagcgcc cgaacagcag cgggatcgcc
    30241 accatgtact gaatgcagtg gtcgcggtca gccgggttat tgagcggccc ttttttgtcg
    30301 atgatgcgaa tacaggcttc gtgggtgcga atggtcactt tttcgatatc cgccgccgtt
    30361 ttgcctgcta cctgcatctg ttcatagagc gtcatcgccg cttcaactgc cgtctgggag
    30421 tggaactccg ccgggaagga gattttgaac agcacatttt ccatgacgta agaaccgtac
    30481 gggcgctgga agcggaacga ttcacctttg aaggagacgt cgtagaagcc ccacacaggc
    30541 gcagtcaggg ctgacgggta acccatttcg cccgttttcg ccatcagtgc cagacgtacc
    30601 gcgcgggaag tggcatcgcc cgccgcccag gatttacgcg tgccggtgtt cggcgcatgg
    30661 cgataggtgc gcagcgactg accgtccacc cacgccagcg aaacggcgtt gagaatttcc
    30721 tcgcgggtca ggccgagcat ttcggcgacc acggcggtgg aagccacttt cactaacaga
    30781 acgtggtcga ggccgacgcg gttaaaggag ttttccagcg cgatgcagcc ctgaatttca
    30841 tgggctttga tcattgcggt cagcacctgc ttcatggtca acggcgcttt gccgctagcg
    30901 accgcgttac gcgaaagcca gtccgccgtt gccagaatgc cgccgaggtt gtcggaagga
    30961 tggccccact ccgccgccag ccaggtatcg ttgaaatcga gccaacggat catcacgccg
    31021 atgttaaatg ccgcctggac ggggtcgagc tggaactgag ttccggggac gcgcacgccg
    31081 ttagggacga cagtgccagg aacaattggc cccagcagtt ttttacaggc cgggtattcg
    31141 agagcttcca gaccgcagcc gagagtgtcg agcaggcagt aatgtgcggt gtcgtaggcc
    31201 actttagagc taatttcgta gttcatcacg taatcgacga tatcaacgat ttcacgatca
    31261 aattccgggc ggatgttgtt gatttgagct gacattgggt acgtttcctt attgttattc
    31321 gtagaggttt actggcgctt atccagcgcg acaaactggc ggtcttccgg tccaacataa
    31381 ttggcggaag gacggataat tttgttgtcc tgacgttgtt cgataatgtg cgccgcccag
    31441 ccggtgacgc gggcgataac aaacagtggt gtgaacatct cggtgggaac gcccatcatg
    31501 ttgtaggaaa cagcggagaa ccagtcgaga ttggggaaca tctttttgct ctcccacatc
    31561 accgtttcca ggcgatcggc gatgttgtac atcttcagcg aaccgccttc ctgcgagagc
    31621 tgcttcgcca cgcgtttgat cacctgatga cgcgggtcgg cgatggtgta aaccggatga
    31681 ccaaaaccaa tgaccacttc tttgttttcc acgcgcttgc ggatatcggc ttcggcttcg
    31741 tccggtgttt cgtagcgttg ctggatctcc agcgacactt cattcgcccc gccgtgtttt
    31801 ggcccgcgca gtgcgccaat cgcgccaata atcgcggaat acatatcaga gccagtgccc
    31861 gcaatcaccc ggctggtaaa agtggaggcg ttaaactcgt gctcggcgta cagcaccagc
    31921 gaaatatgca tcgccttttc ccagctttgc gacggctttt cgccatgcag cagatgcagg
    31981 aagtgaccgc cgatggaatc gtcatcggtt tctggctgga tgcgttcgcc gttgtggctg
    32041 tagtgatacc agtagagaag aatcgagcta agcgacgcca acagtttgtc ggcaatatcc
    32101 cgcgcaccag agacggtatg accctctttt tctggcagcg tgcaaccgag cgcggagacg
    32161 ccggtgcgca taacatccat cgggtgcgac gccgcgggta aggcttccag cacggtacgc
    32221 acgttagctg gtaaaccgcg cagggctttc agtttcgttt tgtaggcggc gagttcgtca
    32281 cgggtcggca gtttgccgtg gatcagcagg tgcgccactt cttcaaattc gcaatgttcc
    32341 gccagatcaa gaatatcgta gccgcggtaa tgcaggtcgt tgccgctttt acccacggtg
    32401 cagagcgccg tattgcccgc cggaacgccg gatagtgcca cagatttttt cggtttaatg
    32461 acatgggtac tgttttgcag gatcgttgtg tcgctcatgt tgtcctcgtc atttgtaggg
    32521 tcgagatatt tttcgttgtg ttttatttcg cctggctacg ggcaaacagg tcgtcgagct
    32581 tctcttcgta ctggtagtag ttgatgcttt cgtacagctc gttgcgggtt tgcatggtgt
    32641 cgatgacact tttctgcgtg ccttcctgac gcaggacgtt gtagacatgt tcggcggcgc
    32701 ggttcatggc gcgaaacgct gaaagcgggt acagcgccat tgcaacatgg gcgctgcgta
    32761 attcgtcggt ggtaaacagc ggtgttgcgc caaattcggt gatgttggcg aggatcggca
    32821 cctgcaccgc atcggcaaac tggcggtaca tggcgagttc ggtaatcgcc tccgggaaca
    32881 acatctcggc acccgcttca acataggcct gcgcacgctc gatcgccgca tccagcccct
    32941 ctaccgccag cgcatcggtg cgcgccatga tcacaaaatc aggatcggtt ttcgcatcca
    33001 ccgccgcgcg gatccgatcc accatctcct ctttcgagac gatcgctttg ttcggacgat
    33061 gaccgcagcg tttcgcacca acctgatctt caatatgcag tcctgccgca ccggctttaa
    33121 tcatcgattt cacggtgcgc gccacgttaa aagcagaaga accaaaaccg atatccgcat
    33181 ccaccagcag cggcagcgaa caaacgtcgg tgatacggcg aatatctgtc agcacgtcat
    33241 caagggtaga aataccgaga tccggcagcc ccagcgaacc tgccgccacg ccaccgccgg
    33301 aaagataaat cgcctgatat ccggcacgct gcgccagcag cgcatggttg gcgttaatgg
    33361 tgccaacaat ctgcaatgga ttttctttag tcagtgcagc gcgaaacgct ttacctggag
    33421 agtgtagaga catagcccat cctttgttat caacttgtta tttgcgttga taaacacaaa
    33481 gcaaggggtg tgccacaatt gccttaataa aattaaatgt ttaatttcaa tttgttagca
    33541 atatagtcat gaagtaatcc gcaaatatgc gtttcagtta acgtttcagg caatgtttca
    33601 ccgcgtttca ttgcaacaat tatgaaacaa gactaaaccc aatattcggt ttcttaactt
    33661 tgcggtgcgc tatggcacat ccaccacggc tgaatgacga caaaccggtt atctggacgg
    33721 tatctgtaac gcgcctgttc gagctgtttc gcgatatcag cctcgagttt gatcatctgg
    33781 cgaacattac ccctatccag cttggctttg aaaaagcggt gacctacatc cgcaagaaac
    33841 tggctagcga acgctgcgac gccatcatcg ctgctggctc taacggcgcg tacctgaaaa
    33901 gccgcctgtc agtgccagtt attttgatta aaccgagcgg ctacgatgtg ttacaggcac
    33961 tggcaaaagc cggaaaactc acctcttcta tcggcgttgt gacttatcag gaaactattc
    34021 cggcgctggt ggcgtttcaa aaaaccttta atttgcgcct cgaccaacgt agctacatta
    34081 ccgaagaaga cgcgcgcggg cagattaacg agctgaaagc taacggcacc gaagcggtgg
    34141 tcggcgcggg gctgattacc gatttggcag aagaagccgg aatgaccgga atttttatct
    34201 attccgccgc caccgtgcgc caggcattca gcgatgcgct ggatatgacg cgcatgtcgt
    34261 tacgccataa cactcacgat gccacccgca acgccctgcg tactcgttac gtgctgggcg
    34321 atatgctcgg tcaatcacca cagatggaac aggtacggca gactattttg ctgtatgccc
    34381 gctccagtgc ggcggtgttg attgaggggg aaacggggac gggcaaagag ctggcggccc
    34441 aggcgattca tcgggaatat tttgcccgcc acgatgcgcg acagggcaaa aagtcgcatc
    34501 cgtttgttgc cgtcaactgc ggggcgattg ccgaatcgct gctggaagca gagctgtttg
    34561 gctatgagga aggggcgttc accggctcgc gacgtggagg tcgcgccggg ctgttcgaaa
    34621 ttgcccacgg cggtacgctg tttctggatg agattggcga aatgccgcta cctttgcaga
    34681 ctcgcctgtt acgggtgctg gaagaaaaag aggtcacccg cgtcggcggg catcagcctg
    34741 ttccggtaga tgtgcgggtc attagcgcca ctcactgcaa tctggaagaa gatatgcggc
    34801 aagggcagtt tcgccgtgat ctgttttatc ggctgagtat tttgcgtctg caattgccac
    34861 cactgcgcga gcgggtgacg gatattctgc cgctggcgga aagctttttg aaagtgtctc
    34921 tggcggcgct ctccgccccg ttttctgccg cattacgcca gggattacag gcaagcgaaa
    34981 ccgtgctggt gcactacgac tggccgggca atattcgtga actgcgcaat atgatggagc
    35041 gactggcgct gtttttaagt gtggaaccga cgccggattt aacgccgcag tttttgcagc
    35101 tgctactgcc ggagctggcg cgcgagtcag cgaaaattcc ctccccatcc ttgctgacac
    35161 cacaacaggc actggagaaa tttaatggcg ataaaacagc agcggcgaat tatttaggta
    35221 tcagccggac gacgttctgg cggcggctga aaagctgaac tgccgaatgc agcgcgaacg
    35281 ctttatccgg cataaatttc aatagatgat gatcaccgat aaacttacgc atcggggatg
    35341 tttacatagg attcagaatt acttcttctt ataaatattt gccgtgccgt gaatcttatt
    35401 gtcagtttga ccagaggtca gcaccaacac atcagcccct ttttcatcgg cttttttgat
    35461 caaatcttct ttcgcatctg cggtcgacat ttcattactg gttgaaatat cacctatttt
    35521 ttcatattgc gattcaacct tttcaaattc cgctttggtc attaattctg cggcatagac
    35581 attggtaacg gcaaacgcta acgcaccgac taacatttta gagattattt tcatattacg
    35641 gctccagagt tgttatcaca ctattaccca cccacattat tgggttgatt aataattagt
    35701 tcagtcaggg cataaacgcc agtaaccgga aataagacac tttgtgatct ctctcgcacc
    35761 cgattatttt tggtagaaat atgatgaagt tacttcactt atttatggat gaaatcacta
    35821 tggatccttt gcacgccgtt tacctgaccg taggactgtt cgtgattact ttttttaatc
    35881 cgggagccaa tctctttgtg gtagtacaaa ccagcctggc ttccggtcga cgcgcagggg
    35941 tgctgaccgg gctgggcgtg gcgctgggcg atgcatttta ttccgggttg ggtttgtttg
    36001 gtcttgcaac gctaattacg cagtgtgagg agattttttc gcttatcaga atcgtcggcg
    36061 gcgcttatct cttatggttt gcgtggtgca gcatgcgccg ccagtcaaca ccgcaaatga
    36121 gcacactaca acaaccgatt aacgccccct ggtatgtctt ttttcgccgc ggattaatta
    36181 ccgatctctc taacccgcaa accgttttat tttttatcag tattttctca gtaacattaa
    36241 atgccgaaac accaacatgg gcacgtttaa tggcctgggc ggggattgtg ctcgcatcaa
    36301 ttatctggcg cgtttttctt agtcaggcgt tttctttgcc cgcagtgcgt cgtgcttatg
    36361 ggcgtatgca acgcgttgcc agtcgggtta ttggtgcaat tattggtgta ttcgcgctac
    36421 gcctgattta cgaaggggtg acgcagcggt gagcagataa tttactctct acgtaatcaa
    36481 cctgatttgc cgaatcccga ccatcggcga tgatagcccc cgtaatgcgt ggtccttatt
    36541 tccgataagt ttacttgagt ctttagcttc ccatttcagc ccactctccc ctgtgtgggc
    36601 ttttttcatt gctctgcccc gtcctgaata gccaaacaat ggcaatgttt cactttgcag
    36661 ggatgataat aaggggatct gattcagaag agttatgaaa agttggaccg ccatcgaggc
    36721 ctcgaacccc gtaccataca acaatcctgg ttgtcgtctg ctcttcctga tgagctaatg
    36781 gcggtttttg atatgagcct tacagcatat tttctgtcag gcttgagtgc gatgcgtaag
    36841 atacagaaaa gaccccacac aaacatgatg gaattatgat ttttttcata tgaatgtcaa
    36901 cccacacatt cacaaaacat ctgcatgaga tcatttttta tacaccgtat agctgcgaaa
    36961 ccactgctga tgctttttta agcgaagcgt cgccgatcac aacaaaatat cggcgaaaaa
    37021 ggcggtggaa tgccatttgg catacggata tacagatgaa atgattacct gctttgcgat
    37081 tcactctctg gtggaataac aactcacagg tggaaccctt tctgaactaa agagtgtagc
    37141 ggattttaac gtgcttattt acaggataat atctgtcacc taattcctgg actctattag
    37201 atatccattg atttaatcgc gcatcatctc tgtatgtttt tacgtccaga acagagagca
    37261 ataacatcac cagtgattca ggaaaaacag ggtagatatt ttttaagtgt aataataatc
    37321 catgcgcaag cgcctgctta atgggcgcgt cggtctccgg gatagacgac atttcgcgaa
    37381 gccatttttc cacatgcata cactctttcc ctttagggaa aagtctcagc aaaggcatat
    37441 ggtgttcaag aagaatacat ttgcaatctg ttcgcactga atcgataaac tgcactaatt
    37501 tcttaagccc ggtaaagcgt ccagcctgca catttgcttt aatatcaaga aacgcatcgt
    37561 cctggtcata tacccaaact gtcccttttt gattttcaag tatgcgaaaa acaaacttaa
    37621 ccgggtgatc agggtaatcc atccagtcat aaaacccagg gaaatcatta aactgcacat
    37681 ttatggtgag tgcgttatcg tctgaccact cacagtctcg tacggcagcc atgagatgaa
    37741 tgagtttgtc gtcatcgact aaatcatcac gacaatcata aggataatca taatattgtt
    37801 taatcaaaac cttgaatata tcgacacagg aaaccaggga gcttttgata tcctctggta
    37861 actgattgtt tttaaccgta ttaagtatgc tttgttgcgt atatggcatc aaat
//
LOCUS       DAOTRK010000028        37030 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.028, whole genome shotgun sequence.
ACCESSION   DAOTRK010000028 DAOTRK010000000
VERSION     DAOTRK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.028"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..763)
                     /gene="sseB"
                     /locus_tag="QIE41_001427"
     CDS             complement(<1..763)
                     /gene="sseB"
                     /locus_tag="QIE41_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDS3530329.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQ"
     gene            complement(882..2165)
                     /gene="pepB"
                     /locus_tag="QIE41_001428"
     CDS             complement(882..2165)
                     /gene="pepB"
                     /locus_tag="QIE41_001428"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDS3530330.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2410..2610)
                     /gene="iscX"
                     /locus_tag="QIE41_001429"
     CDS             complement(2410..2610)
                     /gene="iscX"
                     /locus_tag="QIE41_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDS3530331.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2622..2957)
                     /gene="fdx"
                     /locus_tag="QIE41_001430"
     CDS             complement(2622..2957)
                     /gene="fdx"
                     /locus_tag="QIE41_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDS3530332.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(2959..4809)
                     /gene="hscA"
                     /locus_tag="QIE41_001431"
     CDS             complement(2959..4809)
                     /gene="hscA"
                     /locus_tag="QIE41_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDS3530333.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(4826..5341)
                     /gene="hscB"
                     /locus_tag="QIE41_001432"
     CDS             complement(4826..5341)
                     /gene="hscB"
                     /locus_tag="QIE41_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDS3530334.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFSSGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5437..5760)
                     /gene="iscA"
                     /locus_tag="QIE41_001433"
     CDS             complement(5437..5760)
                     /gene="iscA"
                     /locus_tag="QIE41_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDS3530335.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(5777..6163)
                     /gene="iscU"
                     /locus_tag="QIE41_001434"
     CDS             complement(5777..6163)
                     /gene="iscU"
                     /locus_tag="QIE41_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDS3530336.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6191..7405)
                     /gene="iscS"
                     /locus_tag="QIE41_001435"
     CDS             complement(6191..7405)
                     /gene="iscS"
                     /locus_tag="QIE41_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDS3530337.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7517..8005)
                     /gene="iscR"
                     /locus_tag="QIE41_001436"
     CDS             complement(7517..8005)
                     /gene="iscR"
                     /locus_tag="QIE41_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDS3530338.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8275..9015)
                     /gene="trmJ"
                     /locus_tag="QIE41_001437"
     CDS             complement(8275..9015)
                     /gene="trmJ"
                     /locus_tag="QIE41_001437"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDS3530339.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9134..9937
                     /gene="suhB"
                     /locus_tag="QIE41_001438"
     CDS             9134..9937
                     /gene="suhB"
                     /locus_tag="QIE41_001438"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDS3530340.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10082..10936
                     /locus_tag="QIE41_001439"
     CDS             10082..10936
                     /locus_tag="QIE41_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS3530341.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAENAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESCSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11127..12407
                     /gene="csiE"
                     /locus_tag="QIE41_001440"
     CDS             11127..12407
                     /gene="csiE"
                     /locus_tag="QIE41_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDS3530342.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALTGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKVFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12399..13538)
                     /locus_tag="QIE41_001441"
     CDS             complement(12399..13538)
                     /locus_tag="QIE41_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDS3530343.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(13698..14588)
                     /gene="hcaR"
                     /locus_tag="QIE41_001442"
     CDS             complement(13698..14588)
                     /gene="hcaR"
                     /locus_tag="QIE41_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDS3530344.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTV"
     gene            14724..16085
                     /locus_tag="QIE41_001443"
     CDS             14724..16085
                     /locus_tag="QIE41_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDS3530345.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDSIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16082..16600
                     /locus_tag="QIE41_001444"
     CDS             16082..16600
                     /locus_tag="QIE41_001444"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDS3530346.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            16600..16920
                     /locus_tag="QIE41_001445"
     CDS             16600..16920
                     /locus_tag="QIE41_001445"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDS3530347.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            16917..17729
                     /gene="hcaB"
                     /locus_tag="QIE41_001446"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             16917..17729
                     /gene="hcaB"
                     /locus_tag="QIE41_001446"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDS3530348.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            17739..18941
                     /locus_tag="QIE41_001447"
     CDS             17739..18941
                     /locus_tag="QIE41_001447"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDS3530349.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVHILLNNAIEHVVDGENV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAASAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLTD
                     EHIALKSL"
     gene            19038..19460
                     /locus_tag="QIE41_001448"
     CDS             19038..19460
                     /locus_tag="QIE41_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDS3530350.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19508..20380)
                     /locus_tag="QIE41_001449"
     CDS             complement(19508..20380)
                     /locus_tag="QIE41_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDS3530351.1"
                     /translation="MTIYTLSHGPLELDVSDRGGVIEGFWRDATPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRSEDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGTETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPHQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(20392..21486)
                     /locus_tag="QIE41_001450"
     CDS             complement(20392..21486)
                     /locus_tag="QIE41_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDS3530352.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFTLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(21519..22517)
                     /locus_tag="QIE41_001451"
     CDS             complement(21519..22517)
                     /locus_tag="QIE41_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3530353.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22542..24053)
                     /locus_tag="QIE41_001452"
     CDS             complement(22542..24053)
                     /locus_tag="QIE41_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDS3530354.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24076..25059)
                     /locus_tag="QIE41_001453"
     CDS             complement(24076..25059)
                     /locus_tag="QIE41_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDS3530355.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     SDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(25156..28437)
                     /locus_tag="QIE41_001454"
     CDS             complement(25156..28437)
                     /locus_tag="QIE41_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDS3530356.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGIVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIILSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLATRNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKLWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNRVH"
     gene            28555..29748
                     /locus_tag="QIE41_001455"
     CDS             28555..29748
                     /locus_tag="QIE41_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDS3530357.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQGRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(29812..31065)
                     /gene="glyA"
                     /locus_tag="QIE41_001456"
     CDS             complement(29812..31065)
                     /gene="glyA"
                     /locus_tag="QIE41_001456"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDS3530358.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31393..32583
                     /gene="hmpA"
                     /locus_tag="QIE41_001457"
     CDS             31393..32583
                     /gene="hmpA"
                     /locus_tag="QIE41_001457"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDS3530359.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQALPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLGKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(32628..32966)
                     /gene="glnB"
                     /locus_tag="QIE41_001458"
     CDS             complement(32628..32966)
                     /gene="glnB"
                     /locus_tag="QIE41_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDS3530360.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33027..34361)
                     /gene="glrR"
                     /locus_tag="QIE41_001459"
     CDS             complement(33027..34361)
                     /gene="glrR"
                     /locus_tag="QIE41_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDS3530361.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34351..35064)
                     /gene="qseG"
                     /locus_tag="QIE41_001460"
     CDS             complement(34351..35064)
                     /gene="qseG"
                     /locus_tag="QIE41_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDS3530362.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAINTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYNDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35229..36656)
                     /gene="qseE"
                     /locus_tag="QIE41_001461"
     CDS             complement(35229..36656)
                     /gene="qseE"
                     /locus_tag="QIE41_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDS3530363.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLVDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADGAVELENVELAPLVEMV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
BASE COUNT         8718 a        10244 c         9350 g         8718 t
ORIGIN      
        1 tctgtttaaa gtcacgcaaa aaaccacccc agcgacgttc atagaatggc acaatatgtt
       61 cggtaataaa gtggctaatt cctttttccc cttttttcac ctgacaaata tcgatgggtt
      121 catcgccagg taatgtatcg gtcgctacac ttcccgccgc ctgaataatt tcttcgatat
      181 caccatcggc ttcaatgcca ataagtaaat taggctgtgc ctcttcgttc tctttaattg
      241 aacaaataaa agcacgcttc accggcttaa tagttttaaa taaggtggtg agtgaatcaa
      301 tcatttgtgc tggcggctct gcgacttccg ataatatcag cgattcaccg ccctccagta
      361 cttcctggct gctcagcgga tttccttctt caccaatcaa caaactgatt tcacgcggca
      421 taaattcttt accggttggc agtttggcat taaggaagag cgtttcgcca agggtcatct
      481 caaacaatgt gcgaacgggc attacgacaa atgcctgttc gtcttcaacc gcctgttgaa
      541 gtgcttctaa cgaggtaaaa aaaggaatga cgctggtgcc gtcttctttt tcccagtgct
      601 gtaaatcaag cgcgctatct tcaaccacag cctcgccctg cgccgccgta cccggcaccc
      661 agacggtgga ttccagtaga gtacggaaaa aggccgggcg gtgcgccggt tcggttgctg
      721 ctttttccag cagatcttca agttcgtttt ttgtttcgga cataagaacc acaattcatt
      781 caacgttcgg ggcacaagtg cctgatgcgc tacgcttatc aggcctacaa gggaatctca
      841 atctattgga ttttcgcaat tttgtaggcc aaataagacg attacgccgt taacagatta
      901 gcaatcgtgc gcacaccaag tcccgtagca cccgcagacc actgttcaac cggcgcttta
      961 cggtaagtcg ccgagcagtc gatatgcagc cagccttgct gatagttctc aacaaagtgc
     1021 gacaggaagc ccgccgccgt gctcgcgcct gccgggtacg ccgcgcttcc ggtattgttc
     1081 agttcggcaa agttagacgg cagctggctg cggtggaact ccgccagcgg cagacgccag
     1141 aacggttcat tctcctgcgc ggcactcgcc agcaagcgac cggcaagcgc atcgtcaaaa
     1201 ctgaacagcg cgtgataatc attacccagc gcagttttcg ccgccccggt gagggtcgcc
     1261 gcatcaatga tcagttccgg tttctgcgca ctggcatcaa tcaggccatc ggccagcacc
     1321 agacgccctt ccgcatcagt gttcatcact tcaacttttt taccgttgcg ataggtaata
     1381 atatcgccca gtttgaacgc gttgccgctg atcaggttat ccgcacagca gaggaacagc
     1441 ttcacgcgct tgttcagtcc gcgcgtaatg gcaaacgcca gcgccccggt aaccgttgcc
     1501 gcgccgccca tgtccgactt catcgagtcc ataaatgcgg tctgtttgat actgtagccg
     1561 ccggaatcaa aggtgatgcc tttaccaacc aggcacgcgt acactggcgc ttctttatcg
     1621 ccagtcgggt tgtaatccag cgccagcaat accggagaac gttctgaacc gcgtccgacc
     1681 gtgtgcagcc ccatataacc ttgatcacgc agatcttcgc ctttggtgat ccggtaagtc
     1741 acacgatcgc ccgcgacatt gctgatcaga tcaacagcac gctgtgccag ttgcgatggc
     1801 cccagttctt ccgccggtgc gttgatggtg tcacgcaccc agtcgatgat catcaggcgg
     1861 ttatccagtt cctgccgttg ggcatcgtcc agatccggcc actccacttt acgtgtgcct
     1921 ttcggggctt tgtaaccttg ccagaatgcc cagcagcgat ccgcatccca gtcttcaccg
     1981 cttaactgaa catgcttgat gcccagaccg tcaatcttgc gcgccgcacg ctggatcagc
     2041 cccagatcgt ctgccccgtt caaatgcagg gtaatgccat cattattaat gctgtaagtc
     2101 gctttttctc cccagcgcgc gtcggcaggt tgggtagaga gggtaatctt catcgcttct
     2161 gtcattttag ttatccttct ttgtaaaagg gccgccagtt ggcagcccca ggatttttcc
     2221 gctaaatgat tcgcgttgca ggacaaaacg cattgcgttt tgaacagcgc ttgcgctggc
     2281 cccaaagggg tgaggccaaa aggccgaatc acgcggcaag tgagtgaagc cccaggagca
     2341 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
     2401 acgcggaaat tattcggctt cgtccagcca gactaacaaa atcgcttcga ggattttctc
     2461 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
     2521 ggtgaatcga accgttttcg gatcaagatc gggatacgca tcgtacagtg cttcgccaat
     2581 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
     2641 ttgatagtgt aacgcgggat ttcgaccact aaatcttcgt cggtaacgcg cgcctggcag
     2701 cttaaacggc tttccggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
     2761 gagctttccg gcagcgagtc aaaaccttcg cgaacgatgc agtggcaggt ggtgcaagca
     2821 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagcgcaac gtcgagaatg
     2881 gtttcaccac tattcgcttc cagaacagcg ccatcagggc agagatcctg atgaggcaaa
     2941 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
     3001 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
     3061 ttgttcgata gcgtcaacat catcgccctg cgccacttca ctcaggtgag cggcagcatc
     3121 gtcaatgacc tgacgctctg cggtgcttaa cagcgcggca tcagcagcca gcgcgccgtg
     3181 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcatac gggcttttac
     3241 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
     3301 accataagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
     3361 cacgctcaaa agaccgtcgg catcgacctg gaacgttacg cgaatatgcg cgccgccagc
     3421 cggtagcgcc gggataccac gcagtgcaaa acgcgccagc gagcggcagt cctgcaccag
     3481 ttcacgttca ccctgcatca catggataga cattgccgtc tgaccatctt taaaggtggt
     3541 gaaatcctgg gcgcgggcca ccggaatcgt ggtattacgc gggatcactt tctccaccag
     3601 gccgcccatg gtctcgaggc ccagcgacag cgggatcaca tccagcaaca gcatttcgct
     3661 gtccggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
     3721 atccgggtcg atagaagtca gcggtggacg accgaaaaat tcgcccaccc gttcacgcac
     3781 cagcggcacg cgggtagaac cgccaaccat caccacttcc agcacttcat cagcttctac
     3841 acccgcgtct ttcagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
     3901 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
     3961 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
     4021 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatataat ccgccagcag
     4081 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gtcgccagca cttcaaacac
     4141 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccgccgaggt cataaacagc
     4201 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
     4261 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
     4321 ggcatcgtca aagtacgccg gaacggtgat aacgacacca tccagctcgc ctgccagggc
     4381 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
     4441 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
     4501 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc gtttaacaga
     4561 actaatggtg ttggcggtat cgagcgcggc gttagtacgc gcgtcataac ccaccgaatg
     4621 cccttgctgt tgatagtgaa caacagacgg cagcaggtga cggccttcat ggtcggctaa
     4681 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
     4741 accggccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
     4801 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
     4861 cactgcttcg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
     4921 acgtctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
     4981 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagtt
     5041 cttcgcgcag ttccagctgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
     5101 tggcgagatc aaaaccgtgc aaagaaagca aatattccgc gcgcattaac ggatgacgca
     5161 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
     5221 ggcttccgct ggagaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
     5281 tcagcgcctg ggtatcgagt tgataacagg caggcaagcc aaagagggtg aagtaatcca
     5341 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
     5401 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
     5461 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
     5521 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
     5581 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
     5641 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
     5701 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
     5761 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
     5821 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
     5881 cagcaatatc ggtgtttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
     5941 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
     6001 cttcaatgat accttcatcg ttgactttaa tctgcaactt cattacgtcg ccacaggccg
     6061 gtgcacccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
     6121 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
     6181 ccgataccga ttaatgatgt gcccattcga tgctgttcag atccacgccc tgcttgtaca
     6241 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
     6301 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
     6361 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
     6421 tacaggcgga acctgaagaa accgcgaggt ctttcagcgc cataatcagc gactcacctt
     6481 caacgtagtt gaagctgacg ttgagaatgt tcggcgcgcc gtgttccagg tcaccgttca
     6541 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
     6601 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcttcgccc ataccaacga
     6661 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
     6721 gcgcttcgat gcgtacgcgc ggtttacgac gcacatacag cgcaccgata cctttcgggc
     6781 catagatttt gtggccggag aatgacatca ggtcaacttt caactggctc aggtcgatag
     6841 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccgcga gcacggcaca
     6901 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
     6961 aaacgaggat ggtgtcgtca cgcatcgctg cttcaagttc tttcaggtcg ataatgccat
     7021 tacgctgcgg tgccagatag gtgacttcga aaccttcgcg ctccagctga cggcaggtat
     7081 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
     7141 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
     7201 tttcacgcgg gtcagcgccg accagatctg caatctgatt acgggcgata tcaaccgctt
     7261 cttcagcctg ccagccgaaa cggtgagaac gggaggccgg gttaccaaag gttccgtcca
     7321 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
     7381 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
     7441 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
     7501 ttctgaatac tttttattaa gcgcgtaact taacgtcgat cgcgtcttgt gtgcgggtgc
     7561 gtggcgcgtc gtgagtatgc tgacgaccag acacatccag cacttcctgg ttattaacca
     7621 gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg gtcgctcaaa tcacgccaca
     7681 gcgcgtgggt caggcattta tcgccgccct ggcagccgcc tttaccctga caacgggtgg
     7741 catctacaga ttcgtcaacg gcgctaatca cttcgccaac ggcgatgctg ctggcatctt
     7801 tgcctaacag ataaccaccg cctggtccac gtacgctgga aaccagacca tttttacgca
     7861 gacgggaaaa cagttgttcc agataagaaa gggaaattcc ctgacgttcg gaaatatcag
     7921 ccaacggtac cgggcccgct tcagagttga gcgcaacgtc aagcattgcg gtcacggcat
     7981 agcgcccttt agatgtcagt ctcatgtctt acttcacctc aaactcgccc ctgcccgggg
     8041 ttttttattg taaaagtggg ggtattgcat agcagggtca agtctgacat tcccgagtaa
     8101 attggtcaac tatttacttg actgatttag tcgggtattt aaccttcagt gccatttttt
     8161 tatcgtggcg tttgtacctg ttgtcggatg cgacgtgaac gccttatccg acctgaggct
     8221 ctgtgcactg caggcctgat aagacgcgtt aagcgtcgca tcagacaacc actattattc
     8281 ggccttatta cctttattct gctgctctat agaagccaga atcccgcgca ggatattcaa
     8341 ttcctggctt tccgggcgcg cacgggtaaa caaacgacgc aatttattca tcacctgccc
     8401 cggatggttt tcacgaataa aaccggttgc cagcagcgtt tgttccagat gaccataaaa
     8461 acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgactt gttcgccatt
     8521 ttcctgagtt gccagccagg ccatgcgcac ttcataggca ataacctgaa ccgccatcgc
     8581 cagattcagc gaactgtatt ccgggttagc ggcaatcgcg acatgataat ggcatttctg
     8641 caactcttca ttggtcaagc cgacgcgctc gcgaccaaaa accagcgcca ccggggtatt
     8701 tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg gccacggcag
     8761 cgtgcgggag cgtgcgctgg tgcccaccac caggctacaa ccggctaacg cttcgtcgag
     8821 cgtatcgacg atgtgagcat taccaatcac atcgctggcc cctgctgcca gggcaatcgc
     8881 ctgggagtca ggtttcacca gtggattaac cagccacaga ttggttaatc ccattgtttt
     8941 catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca gcacaattcg
     9001 aatattttgc agcattgtct ttcttcgtct aaagattatt cgcgcatctt atcataaaac
     9061 gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct ttaacatcca
     9121 gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg
     9181 taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc agaaaggcag
     9241 taacgacttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg acacgattcg
     9301 taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg aaggtactga
     9361 tcaggatgtt caatgggtta tcgatccact ggatggcact accaacttta tcaaacgtct
     9421 gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcactgaag ttgctgtggt
     9481 ttacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg cacagctgaa
     9541 cggctaccgt ctgcgcggca gcaccgctcg cgatctcgac ggtactattc tggcgaccgg
     9601 cttcccgttc aaagcaaaac agtacgccac cacctacatc aacatcgtcg gcaaactgtt
     9661 caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg cttacgttgc
     9721 tgcgggtcgc gttgacggtt tctttgaaat cggtctgcgt ccgtgggatt tcgcggcagg
     9781 cgagctgctg gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg gtcataacta
     9841 catgctgacc ggtaacatcg ttgctggtaa cccgcgcgtt gttaaagcca tgctggcgaa
     9901 catgcgtgac gagttaagcg acgccctgaa gcgttaatga ctctggcggg tgatgtcact
     9961 cacccgccct cgcctttcag gcgctattcc gaaatacttc ctcaccgctt tactttcttt
    10021 cgccactctc ccaccatctt tccccgatta atggatgata aagaagtcgc aaggaaaggt
    10081 tatggcgctg ccagtgaaca aacgcgttct caaaattctg tttattctct ttgttgttgc
    10141 cttctgcatt tatttaatac cgcgcatcgc catcaatttt ttctattacc ctgacgacaa
    10201 aatctacggc cccgatccct ggtcagccga atcagtagaa ttcaccgcta aagatggcac
    10261 tcgcctacat ggttggttta tcccttctgc aactggcccc gctgaaaatg ccattgccac
    10321 cattattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc tggtcagttg
    10381 gttacccgag cgtaatttca acgtttttat gtttgattat cgcgggtttg gtaaatcaaa
    10441 aggcacgccg tcccaggccg gattgctgga cgatacgcaa agtgccatca atgtggtgcg
    10501 ccatcgcagt gatgtaaacc cacaacggct ggtgctgttc gggcagagta ttggcggggc
    10561 gaatattctg gatgttattg gtcagggtga tcgtgaaggc atacgtgcgg tgatcctcga
    10621 ctccacattt gcctcttatg cgaccatcgc caaccagatg atccccggca gtggctactt
    10681 acttgatgag agttgcagcg gcgaaaatta catcgccagc gtcagcccga tcccgctttt
    10741 actgattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa agttgtatag
    10801 cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca ttgatgcttt
    10861 ttccgatcgt cacggcgatg tttatcgcga acagatggtg gattttatcc ttagcgcgtt
    10921 gaatccgcag aactaaccca tgatcgctag cacgataatc attcacaaaa ccaccttaag
    10981 acatgctaat ccactggtca gaacagttta agatgagaaa aattctgtga cgcttgccaa
    11041 catttctgat gattagcatt cccttcgcca tttccatgag caaactttag ctattcttat
    11101 caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca tctgtccttt
    11161 cggctcccca gcgccgctgc cagatcttgc tgacactctt tcagccgggg ttgaccgcca
    11221 ccacggcaac tttcagcgag cttaatggtg tggatgatga tattgccagt cttgatatca
    11281 gcgaaacagg acgtgagatc ctgcgctatc atcaactcac actgactact ggttatgacg
    11341 gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt cactggctac
    11401 gacgtggttt ccgtctgtgt ccgtcattta ttaccagcca tttcaccccc gccctgaaga
    11461 gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat ctacaagcgt
    11521 tagtgaatct ctgctcccga cggctgcaaa aacgctttga aacacgcgat attcatttcc
    11581 tgtgtctgta tctgcaatat tgtttgctgc aacaccacgc tggaattacg cctcagttta
    11641 atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg caggaaattg
    11701 gacgccactg gcagcgtcgg gcgctccagc ctgtaccgcc tgatgagcca ctgttcatgg
    11761 cattactttt ttccatgttg cgggttcccg atccactgcg ggatgcgcat cagcgggaca
    11821 gacaactgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc
    11881 gtttttatga tgaacagggg ttgtgcgatc agctttatac ccacctcgcc caggcgttaa
    11941 atcgcagttt gtttgccatc ggtattgata acaccctgcc ggaagagttc gcccgcctgt
    12001 atccacgcct ggtgcgcacc acccgcgcgg cgctgaccgg atttgaaagt gaatacggcg
    12061 tccatctttc tgatgaggaa agcggtctgg tcgcggtgat ttttggtgcc tggctaatgc
    12121 aggaaaacga cctgcatgag aaacagatta ttctactgac cgggaatgat agtgagcgag
    12181 aagcgcagat tgagcagcag ctacgcgaac taacgttact gccgctcaat attaaacata
    12241 tgtcggtaaa ggtatttttg cagacaggcg ctccgcgcag cgcggcactg attattgctc
    12301 cttataccat gccgttaccg ctcttttcac caccgctgat ctatacggac ctgacgttga
    12361 caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg aacaactttc
    12421 gggcgcaaaa acattgccgg aagcgccacc agcgccataa cccagaacac gccgtggccc
    12481 agatattgat acaggaaacc ggcgaaaacg gtcatgatcg caatactgcc gcccatcgcg
    12541 acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc ggcaatatag
    12601 cgcatggcgg ccaggtggca gaccgtgaag gtgccacaat gcagaatttg caccactatc
    12661 aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc gcaaatcgct
    12721 gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact cagcgcaaag
    12781 ataatgactt ccgccaccac gcccagcgac cacaaatagc ccaccgccga ggccgagtag
    12841 ccagcagcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgcgc cccctgcaat
    12901 aaacaaacgc aggccagaaa gcgccagttc tggcgaacca acgccaacca cgcagaccaa
    12961 ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg gatgagaaag
    13021 ccgagcagca tggatgccac gcccaacgtc aacagcgcga ggatcacccg ataatcaaac
    13081 atactgacca gtttgcccgt cagcgccgag ccaatgacaa acgctaccga gccccacagt
    13141 cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag tgcatcggtc
    13201 aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat cagccacgct
    13261 acgtgcgccc ccgcccagaa tgcgacagca aagagaagtg tcagcagtgc cagcacgcgc
    13321 aaggcggaaa tcaggcgaga aggatcgctg acgcggggcg cgatgagcaa actcccgaga
    13381 aaacgggcaa ccaaacctgc ccccaataac aggccgatgg tttctggcgt taaaccaatc
    13441 cccttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa
    13501 tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg tcaggaggcg
    13561 ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa taagaatatt cgagcctgct
    13621 tcccgatgtc tgtaacacca gttcacaagc actaaaaaac tgcgttccgc gacgcatgtt
    13681 gcaaaaataa tgcgtaatta taccgttacg cttgccaaac gttcctgcac aatggcgatg
    13741 aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa agcaatggaa
    13801 ggcacatttc cggcaatggg tcgaaatacc acctggccgg tattaaaatt attcatataa
    13861 ccgggtatca aagtgacgcc cagccccatc cccaccagat tcatggtcac cagaatattc
    13921 gttgccacct ggacgatatt tggctggctg ttttcttgcg caaaccacgc tttaacgatc
    13981 ggcgcaagcg aaccggaata cgctggatca gtactgacga aattcacgcc atccagttgc
    14041 gcggcagtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac caccactaat
    14101 ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg gcgcattaag
    14161 ccgacgtcga gttcaccacg gcgaattttt tcttcctgtt ccgtggtgat taaactcacc
    14221 agctcaatta aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt tggcagtaaa
    14281 ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg aacaattttc
    14341 cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa
    14401 cattctcccg ccgccgtcaa cgcgactttg cgcttatccc tcaccagtaa cggaacaccg
    14461 acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt atgcagtttt
    14521 tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg taaatgccgt
    14581 agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa attaactatt
    14641 tcacatatta gcaaccaacc agcaacatcc ttatggcaca aaaatagaag gtcaatacat
    14701 cttatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt taccaactga
    14761 ttgacaccca aaacggtcgg gtcactccgc gtatttatac cgacccggat atttaccaac
    14821 tggagcttga gcgcattttc ggtcgttgct ggctatttct cgcccacgaa agccagatcc
    14881 caaaacctgg tgatttcttt aacacctaca tgggggaaga tgcggttgtc gtagtgcgtc
    14941 agaaagacgg cagcattaag gcgtttctca accaatgccg ccaccgggcc atgcgtgtga
    15001 gttatgcaga ttgcggcaac actcgcgcct ttacctgtcc gtatcacggc tggtcttatg
    15061 gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca caagggttgt
    15121 gtaaatccca ctggggacta aacgaagttc cttgtgtgga gagttataaa gggctgattt
    15181 ttggcaactg ggataccagc gcaccaggcc tgcgtgatta cctgggtgac attgcctggt
    15241 atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc ggtgtacaaa
    15301 agtgggtgat caactgcaac tggaaattcc cggcagagca gttcgccagt gaccaatatc
    15361 atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat gatggcagcg
    15421 ataagcgcct cggtgatgga caaaccgccc gtccggtatg ggaaaccgcc aaagatgcgc
    15481 tgcaatttgg tcaggacggt cacggtagcg gtttcttctt tactgaaaaa ccggatgcta
    15541 atgtctgggt cgatggcgca gtttcaagct attaccgcga aacctatgcc gaagcagaac
    15601 aacgtttagg tgaagttcgc gccctgcgtc tggcgggtca taacaatata ttccccacgc
    15661 tttcatggct aaacggcact gccacactcc gcgtctggca tccgcgcggc cctgatcaag
    15721 ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt aaagccgctt
    15781 tcgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag gatgactcgg
    15841 agaactggtg tgaaatccag aaattgctga aaggtcaccg cgcccgcaac agcaaactgt
    15901 gtctggaaat ggggcttggt caggaaaagc gtcgcgacga cagcattcca ggcattacta
    15961 actatatctt ctcagaaacg gccgctcgtg gaatgtacca acgctgggcc gatctcctga
    16021 gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag gaggtgatga
    16081 aatgagtgcg caagtttcac tggagttaca tcaccgcatt agccagtttc tctttcacga
    16141 agccagtctg ctggatgact ggaaatttcg tgactggctg gcacagctcg acgaagagat
    16201 tcgttacacc atgcgcacca cggttaacgc acaaacgcgc gaccgccgca aaggcgttca
    16261 accgccgaca acctggattt ttaatgacac caaagaccag ctggaacggc gaatcgcccg
    16321 tctggaaacc ggcatggcct gggcagaaga accgccgtca cgcactcgtc acttaatcag
    16381 caactgccag gtaagcgaaa ccgacatccc aaacgtattt gccgtgaggg tgaattatct
    16441 actttatcgg gcacaaaaag agcgcgatga aacattctat gttggaacgc gtttcgacaa
    16501 agttcgccgt ctggaggacg ggaactggcg cttgctggaa cgggatatcg tcctggatca
    16561 agcggtcatc tcctcccata acctgagtgt actgttctga tgaatcgaat ttatgcgtgt
    16621 cccgttgcgg atgtgccgga gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc
    16681 ctgttcaacg taggcggcga gttttatgcc attaacgatc gttgcagcca tggtaatgcg
    16741 tcgatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct gcacgccgcc
    16801 agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc gctaaccact
    16861 tatccagtac acgttgaagg tggtgacatc ttcatcgact taccggaggc gcagccatga
    16921 gcgatctgca taacgagtcc atctttatta ccggcggcgg atcgggatta ggcctggcgc
    16981 tggtcgagcg atttatcgaa gaaggcgcgc aggttgccac gctggaactg tcggcggcaa
    17041 aagtcgccag tctgcgtcag cgatttggcg aacatattct ggcggtggaa ggtaacgtga
    17101 cctgctatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttct ggcaagctgg
    17161 attgttttat cggcaatgcg ggcatctggg atcacaatgc ctcactggtt aacactcccg
    17221 cagagacgct cgaaactggc ttccacgagc tgtttaacgt caacgtactc ggttacctgc
    17281 tgggcgcaaa agcctgcgct ccggcgttaa tcgccagtga aggcagcatg attttcacac
    17341 tgtcaaatgc cgcctggtat cccggcggcg gtggcccgct gtacaccgcc agtaaacatg
    17401 ccgcaaccgg acttattcgc caactggctt atgaactggc accgaaagtg cgggtgaatg
    17461 gcgtcggccc gtgtggtatg gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg
    17521 aaacctcgat aatgcagtct ctgacgccgg agaaaattgc cgccatttta ccgctgcaat
    17581 ttttcccgca accggcggat tttacgggtc cgtatgtgat gttggcatcg cggcgcaata
    17641 atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcgatt cgcggcattc
    17701 gccacgtagc ggctgggctg gatctttaag gaagcacgat gaaagaaaaa acgatcatta
    17761 ttgtcggtgg cgggcaagcg gcggcaatgg ctgcggcctt gctacgccag caagggttca
    17821 ccggtgagct gcatctgttt tccgatgagc aacatcttcc ttatgaacgc cccccgctct
    17881 cgaaatccat gttgctggaa gattccccac agttgcagtc tgtgttaccc gctcactggt
    17941 ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacattg ggccgcgaca
    18001 cacaagagtt agtgttagct aacggcgaaa gctggcactg ggatcagctt tttatagcaa
    18061 ctggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc tgctttaccc
    18121 tgcgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa cggtcagtcg
    18181 tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg cagcgcggat
    18241 gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg tcgtaatgca ccaccgcccg
    18301 tacaacgcta tcttttgcag cgccatcagc aggctggtgt gcacattctg ctcaataatg
    18361 ccattgaaca tgtggtcgat ggtgaaaacg tagaactgac gctgcaaagt ggcgaaacgc
    18421 ttcgggccga tgtggtgatt tacggtattg gtatcagcgc caacgaccaa ctggctcgcg
    18481 aggccaacct tgatactacc aatggcattg tcattgatga ggcttgccgc acctgcgatc
    18541 ccgcgatctt tgccggtggc gatgtggcaa tcacccgtct tgataatggt gcactacatc
    18601 gctgcgaaag ctgggaaaac gccaataacc aggcgcaaat tgccgcttcc gcaatgttgg
    18661 ggctaccgct tccgcgactg ccgccgccgt ggttctggag cgatcagtac agtgataact
    18721 tacagtttat tggtgatatg cgtggtgatg actggctttg tcgtggcaac ccggaaactc
    18781 agaaggcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggta acgctgaatc
    18841 aggggcgtga gattcgctta atccgcaaat ggatccagag cggcaaaacg tttgatgcga
    18901 aactgctgac agatgagcac atcgcgctta aatcactgta accaggataa ttagcgaata
    18961 tctcaatgcc tggggcgtgg cgaggtgcca gagtgtgtat tacgtttaaa tcacattatc
    19021 ttgcaaaggg attggttatg aacacactac gttattttga ttttggagct gcccgccccg
    19081 ttttgttatt aattgcccgt atcgccgtgg tcttaatttt cattattttt ggttttccca
    19141 aaatgatggg ttttgacggt acggtccaat atatggcctc gctgggcgcg ccaatgccga
    19201 tgctggcagc gattattgcg gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg
    19261 gctttttcac ccgtccgctg gcggtgctgt ttattttcta cacgctgggt acagcggtaa
    19321 ttggtcacca ttactgggat atgaccggcg atgcggttgg gccaaatatg attaatttct
    19381 ggaaaaatgt cagtatcgct ggcgcattcc tgctgttggc aattaccggg ccgggggcaa
    19441 tttctctcga tcggcgttag gaaagatgcc ggatgcggcg tgaacgcctt atccggcatt
    19501 ttataaatta cagtaacgca acccgcaacg acatctctga agttgttgat tcccctggtg
    19561 caagcgcaat gagatcaccc ccttccgggc gatgatggtc atccggcgca tggctcatcg
    19621 gttccagaca gaagaaatca aacgcatatc ctttatcaaa cgccgggtcg gaaacaaaga
    19681 tgaaataaca tggtgcaggt ggcgtcgttt ccatgatgat ggcataaccc tcctgcggct
    19741 gctcgatgcg ggcctgacca ttccatccgg caaagccatt gttcacccac tggtgcggca
    19801 acggcgcgag ctggttgaaa tccagctcct gcggtagctg ctcgcaaaac tcacccgcca
    19861 gccactgctc ccgctccagc cagtaaccgc tcgcctgcgc ctgaatccgc gtttgtggtg
    19921 acaacggaaa ataaggatgc cagccggtac caaatggcag cgtctctgtc ccttgattgg
    19981 tgacagagag cgtcaccgtc agcgtatctg ccgttaaatg aaacgcctga cttacccgat
    20041 agtgataaac accgctgcga tgttcataca ccagacacaa gctatcttcg ctgcgcgaga
    20101 cacattgcca ctgccccagc cagccgtcgc cgtgcagata gtgcgcatcc cactcaacat
    20161 tcggttgcag ttgatactca cgcccctgcc agacaaaacg attgccgctc acccggtttg
    20221 caaacggcac cagcggaaaa caggaggcat cggttgccac accgcttttt ttaccgggac
    20281 gtaagagcgg cgtcgcgtca cgccagaagc cttcgatgac gccgccccgg tcagaaacgt
    20341 ccagctctag cggcccgtga gataacgtat agatggtcat ccgctgactc cttaatccgg
    20401 gaagttaatc acaactttcc cgcatttacc gctcgccatc agcgcatagg catctcctgc
    20461 ctgttccagc gtgaagcgat gggtaatggc attacgcggc cacagtttcc agtccgttaa
    20521 atcgtgggcg catttttcca tatggaacag actggtcacc caggagccaa taatccgccg
    20581 ttgatgatgc atcagatcgg cgctaacctc gaactccact tttccggttt ccccgatgta
    20641 aaccacccgt ccccagtcag cagtggattg caacgccagt aagcgacctg cggcattacc
    20701 ggaacaatcg agcgcaacat ccgcgccacc gtgggtgagt tcggcgataa tctgcggcag
    20761 accttcggtg gttgctaaat agccgtgatc catcacccct aactgtttcg ccatcgccag
    20821 acgttccggc agcatatcaa cgccgatgat cctttttgca ccgcgacctt tcgccagcat
    20881 catcgccatc atgccgaccg gccccagacc gaccaccagc acgttatcgc tgccggaaac
    20941 ttcgccgcgc aaaatccctt cataggccgt gccaacgccg caactaataa acgcaccatc
    21001 ttcgtagctc agcgcatccg gcaggaggat cagatctttt tcttccgcca gcaagtattc
    21061 ggcatgaccg ccgtcacgct gccagccgta agccgctttt ccttcgccag tacaagaaat
    21121 aggaaaaccg cgacggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac
    21181 gcggtcgccc tctttaaaat ggcggcagcc ttgccccatc gccacaattt gcccgcacgg
    21241 ttcatgaccg ttgataaatc cctggtataa cggtttatcg ggtgccgctg ccgtggcacg
    21301 gtgttgatga tagatatagt ggacatcact tccgcaaatc ccggaggatt tcattttgat
    21361 cagtacctgg ttaatccccg gcgtcggcac cgcaacttcc cgcagatcga cggtcgaatt
    21421 tcctggtaaa taagctgcca gcatcgtttt cataaatccc tcattaacaa tacgattaat
    21481 tttcatccct gccgcacccg cgccaggggc gttaaggttt agcgtttcgc tttactgcta
    21541 cgctgggtca gcaagatatt cgccagcacc gccaccacga tgatgacgcc gcgcaccacc
    21601 tgctggaaaa aggagttaat cccgagcagc accagaccgt taccgattag cgtaatcacc
    21661 aacacaccaa gcaatgtacc aaacagggaa ccgcgaccac cggaaagcgc cgtaccgcca
    21721 accacgaccg cggcgatgac gtcaaactcc agaccgtttg cggcacctgc gttaccggaa
    21781 ccgaggcgcg ccgccaacaa aatgccggtc accgctgcta ataatcccga aagggtaaaa
    21841 ataagaatgc gtacccgacg aacgttaata ccgcacaact gtgctgcagt ggcattaccg
    21901 ccaacagcaa aaaccgagcg cccgaaggcg gttttgcggc taatgaacac aaacagcgca
    21961 aataacacca tcatgatcag cgcggatacc ggcacaccga gaaattgtcc ccccagccag
    22021 tccagcactt cgttttcgtc aatcggcact ggcaaggcgt tcgtcataaa cagccccatt
    22081 ccgcgcaggg cgctccacag ccccagcgtg gccacgaaac ttggcacgtt aaacacgccg
    22141 cgcagcaccc cggcgagcgt tcccatcagc gcgcctaaca gcaacaccag cagacaagca
    22201 atcgccaacg gaacttcaaa ttgcagcaaa aatgccaggc acaccgagac aaaagccacc
    22261 atcggcccaa cgctgacatc aatttcaccg gagataataa tcagcgtcat cgcccaggcg
    22321 gcaatcccaa tggtggcggc atcgcgcagc acgttcatct ggttattcaa tgagataaaa
    22381 cccggcgcgt tcagagaaaa gaccagataa agaatggcga tcaccaccag caaaccgatc
    22441 tcattaatat ggcgactgac aaattgtttg agcgagacgc tcttgccctg cggcaatggt
    22501 aatgacgaag cagacatctt caggttcctc ggtaaaattc atcagtgcac agacagaatg
    22561 gcggacatca gctcatccac attgaccggc gagtgaaact cctgcgagaa cgtaccgtgc
    22621 tgtaataaca ggatgcggtc acacaccagc ggcaactcct ccacttcact ggagataaac
    22681 accacgcttt ttccttcggc agccagctcg cggacaatac ggtaaatctg ctgtttggct
    22741 tcgatatcga cgccacgcgt tggctcgtcg agcaacaaaa tctggctggc ggcatagacc
    22801 caacgaccga tcaccacttt ttgctgattg ccgccagaaa gcgtgccgat gggcgtttcg
    22861 ctactggcgg ccttgaccgt catccgctgc atcacctctt cggtcaggcg gcggatggtg
    22921 gaccattgca gcacaccgtt ggcgctgatt ttttgccgat tggtcagcac tgtattttcg
    22981 tcaacgccca gccagggaat gatccccgct tctttgcggt tttctggcgt atagccaatg
    23041 ccgcgtttca gcatgtcgcc gtaatcgggg cgcgtgattt tctcgccgtt gataacaatt
    23101 tcgccctgtt catacgtctc cagcccaact atggctttca acaattcact gcgccctgcc
    23161 cccagcaggc cagcaatgcc gagtacttcg ccacgacgga gcgtaaagct gatatcctcc
    23221 agcttgggct tatggcgtaa cgcacggact tccagcacgg cctgatcgac aatttcctga
    23281 ggggaaaccg gcgcaatatc aacgtgatcg cgcccgagca ttagcgacac aatatgatgc
    23341 gtggaggtgt tttcgagcat cacatcgccc gccacctgac catcgcgcat aacggtggca
    23401 caggaggcaa tgcggcggat ttcttccatc cggtggctga cataaatcac cgccacgccc
    23461 agtgccgaca tctttttcac cgcgctgatc accagttcaa cttccgcact ggcaagcgaa
    23521 ctggtaggtt catcaagaat gaccacgcgc ggctcgccct tcatcacccg cgcaatttcc
    23581 accagctgct tttgtgccgg gcttagcgtt gaaacaagtt gttcagggct aacgtcaacg
    23641 cccagcgcct gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg
    23701 ttgcggcggg gccactgacc gaggcagagg ttttccgcca cagtcagtcc ctccaccaga
    23761 cttaactcct gataaaccgc acgaaccccc agttcagcgg cacggcgagt caacgtagct
    23821 tcgtcacctt ccagtcgcgt ctcgccaatc cagatatcac cgctatccgg acgttcactg
    23881 ccggtaagca ttcgaatgag ggtcgatttg cccgcgccgt ttttgcctaa cagcgcacga
    23941 acttcgcctt tattgagcgt gaagttaacg ttatccaacg caacgacgcc gggataacgc
    24001 ttatttcctg ccaccacttt tgctaccggg actgcctctg ttgccgtgaa catagggacc
    24061 tctgcgaatc agcgattagg gcagaccatc aacgtgcgtt gccagccact gtttaccgtc
    24121 ttccgttttg gtatagagat cgattggcac ctgaatcact ttttcaccgt cggcttgttt
    24181 attgataacc ttcaaagttt gcgcgaaaac agcattgccc attttcttac cggaaatatc
    24241 caccaccgct ttcagcacct gattgttttc cagctcctga gcaatttcag tggtcatatc
    24301 cgaaccgaaa acagcaattt ttccggcctg attttgatta cgtaccgctt ttaccgcgcc
    24361 gagtgtcgca ccgcccgatt cccccataat ggcgttgaga tccgacgtgg agataattag
    24421 tttttcacca acggaaatcg ctttatctaa aacagtccct tcctgattag cgacaatttg
    24481 cgcgccggga acgcgggctt ttaatacttc ttcaaaccct ttacgtcgtt gcacacaaac
    24541 ttcaaaggct tcgcaattga tgacggcaat tttcggctgg tcaattttgt tggcaataaa
    24601 ataatcggcg gcagcgttac ccagtttttt accaaattcc agcgggtcac caaccagata
    24661 tgccgagaca tatttatcga cacccttttg attaatacag gtgttgtagc aaatcaccgg
    24721 aatacccgct tcactggcgc gacgaacggt acggctactg ccattttcag atactgccga
    24781 taaaataatg gcatcgacat tacgcgccac gagggtatct acaaaagtac tttctttcga
    24841 aatatcgccc tgggcgttag tttcaattaa ctgtacctgt actgaagaat cttttgccgc
    24901 atcctgaacg ccctgacgca cgccagcgta atatccctgg gtatcaaggt atattgcacc
    24961 aatggtcatt tctttatccg ctgccctggc aaatagagcg ctacctaata gcgtagccat
    25021 taataataga ttacgggttg ttctcatttt tttaggcata gtgcctcctg tagggttttt
    25081 atttacaacg gcttattcta attattttgt gatgagcggc agcgcgtgcc gctcaaatat
    25141 tacagttaat aaggtttagt gaacgcggtt aaaaataaaa ggcatcacgg tggtgaataa
    25201 tgctgctttc ggccaggccg gattaatcgc cgtcaattca gcgcgagcag attcatattg
    25261 cgcaacctcc cctagtcccg cggacgccag catcgccacc atcaggcatt tttctttatg
    25321 ctgctgttgt aaatcgccat acagcgacaa caggtcaggt tgcgagacgg caaagaaatc
    25381 cgcttcgata ctggttttcg ccatctcttg cgcccacagt ttcatttcac taaacagttg
    25441 ctgtgcggtg tgttgttcgc ccagcagtcg cagcgccatt ccttgccaga agagataatc
    25501 aaccggctga tcgttgtaat aactgtggat gttaatggtg cgatcgccgg tcgccgccag
    25561 acgtaaacaa cgcgtcgctt cagtttcatc gccctgcgcg ttggcgcata tcgcctgcca
    25621 gaaccagatg tcgttatcag tttgccccgg taaacggcct tcgcttaaat tctccggata
    25681 atgcagcgcg gcatgaagca gttcgctggc ctgctgcggc tctctggcat caagatgctg
    25741 ccaggcgcgt aataactggt tgaggataaa ctgactggtg atcttccctt ccccgccttc
    25801 ccacgggtgg aatttgcgcg tggcgagaat gtccgccgct ttgtctgcct gacccgtgag
    25861 atgccacaaa ttgagcagtt ctgcggtcat gtcgtcgcgt ttcagcgcaa tttccagatt
    25921 attttccaga cgcgccagtc gtttctccgg tgtggctcca cttaacttat ccagcaaatc
    25981 ccgttcgaaa agcagacgtg catcctgcgg cgcaagctga taagcattat caagataacg
    26041 cgcggccagc tcataatcgt gttgcttatt ccacgcatgg atcgctaacc cgcgccaacc
    26101 gtcggcaaac tccggcgaca tctctacgca acgttgccat aaagtaatgg ctttgttgta
    26161 gctgcgcttg ttgtagtaga agcaggccag taaatgacgg gcaaaccagc actcttcaat
    26221 actctccagc gccgccactt cttccagcgt attcgggaaa cggacaaact gcgggaagac
    26281 atcaatggct tttgcgacca gttcgccgcg ttcggctttc ggcagcaagc tggcttgcag
    26341 gtaaagcggc agcgtgcgct ggcagtccag tgcgttcagc atctctgccg ccagggtggg
    26401 cattccccag ttaatcagtt gcccggcggt catcagggcg ttaacgtcgc gtccctgaca
    26461 cagaccgcgc cactgcgcga gggctgattc gctacgacca tcgaaccagt tcagccacca
    26521 cagagtggcg ttcagcggat aatcgcgcag cagtttctcg cgctgcaaac gcgcgttgtc
    26581 ctgacgacca cttaacacca gcagcagatt atgcaggcaa agcacttcct gattggttgg
    26641 gcaggcgcga agactttgtt ggcaaaaatc cagaccagca tcgaagtgac cattacgcgt
    26701 cgccagtcgt gccagaccat aatagccgcc tgctttacta ttgccgctcc agaccgcacg
    26761 ccagaaatcc tcttcggctt gttgatattg tccctgacgt tcgtaagcac tggcgcgaat
    26821 caaactggcc tgtccgcact gcggattttt gttcagcgca tgtgcgcgtt tcagcgcctg
    26881 agtggcatac gccaccgctt gcgggaaatc ggcgcggtta tattccagca tcgccagcgc
    26941 caggttacag cgataatcca gcggatccag cgccacgccg cgcaggtagt aatcgaacgg
    27001 tgaacggctg gcgtgatgat attgctccag atgctgaccg ataaaccagg cttcatctgt
    27061 actggtaatg tcttgtgctg ccagtggcgc tttggcgacg tccggcaacg gcaactcttg
    27121 cggctgatgt tcctgataac tcagtatgat attgccgtcg gcatcggaga gttcaatggt
    27181 cagcctttca ggattgatac cgtgcaacac gccctggatg gcggtcgctg gtgtcagggc
    27241 cacggcatca tcgagtaacg cgttgcattt gccgatttcg cggatcgcca gacggtatcc
    27301 tttcaacgga gagatggcat acagccccca ctcaatcccc cgctcactac gctggagttt
    27361 tatcaccgca tcgcgggagg cattttgcac aatgcccaga gaatgataag gcaggaaata
    27421 ctgctcgaaa cgcttctctt cgtacgcatc aagccaggta aaatcaggct ggttatcggc
    27481 aaaaataccg gtcatcagtt cgatatacgg gccgttattg tcggtcagac tcttatccca
    27541 cgcctggcca aattcactgt gtccccaact ccactgtttt ttacctggcg caatatggtg
    27601 gttggcaacg tgcagcaaac cgccatcttc atcgtgacac cacgcgccaa caaaatcgta
    27661 ctgtgatttt tcagccatat atgaggttgg aacaggcaca tttttatagc gagaaatgtc
    27721 cactccagcg gagtagtcca ctttgtagta agtgccggtg gcgatgggga aagcagagac
    27781 ggcccgtttg ccgtgatcaa acaccgcagt cacatccggt gggaaaacgc tctgatgccc
    27841 ttcaccccct ttcactgccg ggttggccca ccacaagaaa tgacgcggcg tggcgttgcc
    27901 gttatagacg cggctggcga tttccagcgc cgcccggtca ggacgcaggg tgaaacctgt
    27961 catcacctgt aaaccatgca tcggctccgt ttcgcctacc cacacggttt gtgcgccgtc
    28021 ttcatgggct tcgagggtga aatcaacggg cataaaggtg gtcgggcgat ggtgttgcgg
    28081 ccagttaaac tcaatcccgc cggagatcca cggtcccagc agccccacca gcgccggttt
    28141 aatgacttca ttgtgataga caaaatcgcg ctgtttcact ttatcccacg cgcgatgcac
    28201 ccgaccgccc agttccggca ggatcatcac tttgatgtag tcgttttcca gccacaccgc
    28261 ctgccaggat ttcagggttt tctgctcgct cagcgtatcg gtcacgccgt agggataaac
    28321 cgcgccggac gatccctgat aaacgcgatt ttccaggaac atgggatgta tatcctgcgg
    28381 cccggtttca taggtcggga tctcaacgcg ctcttgccac acttttactg gagtcatggt
    28441 gccctcaata ttaacaagac atactgaatt aaaagatttg tggcagtgta ttgaacaatc
    28501 tggcaatgtt ttcgcggaat aatcacgcaa ttaactaaac aaggtttagt gaagatgaga
    28561 gcctgcatta ataatcaaca gattcgccac cataacaaat gcgtgattct ggaactgctg
    28621 taccggcaaa agcgcgccaa taaatcaacc ctggcccggc tggcgcaaat ttcgattccg
    28681 gcggtcagta atattttgca ggaactggaa agcgaaaaac gggtggtgaa tatcgacgat
    28741 gaaagccaga cgcgcgggca tagtagcggt acatggctga ttgcgccgga aggtgactgg
    28801 acgctgtgcc tgaacgtgac gcctaccagt attgagtgtc aggtcgctaa cgcctgttta
    28861 agtccgaaag gcgaatttga gtatttacag attgatgcac cgacgccgca ggcgctgctg
    28921 tccgaaatcg aaaaatgctg gcatcgccac cgtaaattgt ggccggaccg taccatcaac
    28981 ctggcgctgg caatccacgg tcaggttgat ccggtgactg gcgtgtcgca aaccatgccg
    29041 caagcaccgt ggacaacgcc ggttgaggtg aagtatctgc tggaagagaa gctcggcatt
    29101 cgggtgatgg tcgataatga ctgcgtgatg ctggcgctgg cggagaaatg gcaaaataat
    29161 tcgcagggcc gggatttctg cgtgatcaac gttgattacg gtattggctc gtcgttcgtg
    29221 attaacgagc aaatttatcg cggcagtttg tatggtagcg gacagattgg tcacaccatc
    29281 gttaatccgg atggcgtcgt ctgcgactgc ggacgttatg gctgcctgga aaccgtcgcc
    29341 tcgttaagcg cattaaaaaa acaggcgcgg gtatggctaa aatcacaacc ggttaatact
    29401 cagcttgatc ctgaaaaact gacaacagcg cagttaatcg ctgcctggca gagtggagaa
    29461 ccgtggatca ccagctgggt tgatcgctct gccaatgcca ttggtttgag tctgtataac
    29521 ttcctcaaca tcctcaatat taatcagatt tggttgtacg gtcgcagttg tgcctttggt
    29581 gagcactggc ttaataccat tattcgccag acgggattta acccgttcga ccgcgacgaa
    29641 ggaccgagtg tgaaagcgac gaaaattggc tttgggcaat taagccgcgc acaacaggtg
    29701 ctggggattg gctatttgta tgttgaggcg cagttacggc agatttgatg gcgcgataac
    29761 gtagaaaggc ttcccaaagg aagccttttt tcaacagatt aaccgtttcg cttatgcgta
    29821 aaccgggtaa cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga taaccgcttc
    29881 atcattgatg ctgtccagca cgtcacacat ccagccagcc agttctttcg cttctgcttc
    29941 tttgaagccg cgacgggtaa tcgccggtgt acccacgcga ataccggagg tcacaaacgg
    30001 gctcttcgga tcgttcggta cgctgttttt gttcacggtg atgttggcac ggcccagagc
    30061 ggcgtctgct tctttaccgg tcaggttttt atcaaccaga tcaaccagga acaggtggtt
    30121 atcagtaccg ccggaaacca ctttgtagcc gcgctcgagg aacacttcta ccatcgcttt
    30181 agcgttttta gcgacctgct gctggtaagt tttgaactca ggctccatcg cttctttcag
    30241 agcaaccgct ttaccggcga ttacgtgcat caacggaccg ccctgaccac cagggaaaac
    30301 agcagagttc agttttttgt acagctcttc actaccacct ttcgccagga tcaggccacc
    30361 gcgcggaccc gccagggttt tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg
    30421 gttcgggtag acgccagctg caaccaggcc cgcaacgtgc gccatatcaa cgaacaggta
    30481 agcaccgatg ctgtcagcga tttcacgcat tttcgcccag tccaccacgc cggaatatgc
    30541 agagaagcca ccgataatca ttttcggttt gtgttctttg gcttgttttt ccagatcggc
    30601 gtagtcgata tgaccggtag catcgatacc gtaaggaacg atgttgtaca gtttaccgga
    30661 gaagttaacc ggagaaccgt gagtcaggtg accgccatgc gccaggttca tacccagaac
    30721 ggtatcgcct ggttccagca gcgcggtgta caccgcaaag ttagcctggg agccggagtg
    30781 cggctggacg ttagcgtagt cagcgccgaa cagttctttc gcacgatcga tcgccagttg
    30841 ttcaacgata tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc
    30901 atatttgttg gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc
    30961 ggaggcgatc agttcgatgt gctcttcctg acgtactttt tcctgctcca tagcctgcca
    31021 cagttcggca tcataatcgg caatgttcat ttcacgcttt aacatccgca tctcctgact
    31081 cagctaacaa taaaattttt ggcctttata ggcggtcctg ttggacaacg gcgaacagta
    31141 taaccgaatc attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa cgattacctt
    31201 caggctgcgc aaggctttgg agaataaaga gcttgcaacc ggaaacggat ttcttttcag
    31261 gtttgtgatg caaatttttc acttcatcac attctttctg aaaaacacca aagaaccatt
    31321 tacattgcag ggctattttt tataagatgc atttgagata catcaattaa gatgcaaaaa
    31381 aaggaagacc atatgcttga cgctcaaacc atcgctacag taaaagccac catcccttta
    31441 ctggtggaaa cggggccaaa gttaaccgcc catttctacg accgtatgtt tactcataac
    31501 ccagaactca aagaaatttt taacatgagt aaccagcgca atggcgatca acgtgaagcc
    31561 ctgtttaacg ctattgccgc ctacgccagt aatattgaaa acctgcctgc gctgctgcca
    31621 gcggtagaaa aaatcgcgca gaagcacacc agcttccaga tcaaaccgga acagtacaac
    31681 attgtcggtg aacacctgtt ggcgacgctg gacgaaatgt tcagcccggg ccaggaagtg
    31741 ctggacgcat ggggtaaagc ctatggcgta ctggctaatg tatttatcaa tcgcgaggcg
    31801 gaaatctata acgaaaacgc cagcaaagcc ggtggctggg aaggtactcg cgatttccgc
    31861 attgtggcta aaacaccgcg cagcgcgctt atcaccagct tcgaactgga gccggtcgac
    31921 ggtggcgcag tggcagaata ccgtccgggg caatatctcg gcgtctggct gaagccggaa
    31981 ggtttcccgc atcaggaaat tcgtcagtac tctttgactc gcaaaccgga tggcaaaggc
    32041 tatcgtattg cggtgaaacg cgaagagggt gggcaggtat ccaactggct gcacaatcac
    32101 gccaatgttg gcgatgtcgt gaaactggtc gctccagcag gtgatttctt tatggctgtc
    32161 gcagatgaca caccagtgac gttaatctct gccggtgtcg gtcaaacgcc aatgctggca
    32221 atgctcgaca cgctggcaaa agctggccac acagcacaag tgaactggtt ccatgcggca
    32281 gaaaatggcg atgttcacgc ctttgccgat gaagttaagg aactggggca agcactgccg
    32341 cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag ccgatcgcgc taaaggtcag
    32401 tttgatagcg aaggtctgat ggatttgggc aaactggaag gtgcgttcag cgatccgaca
    32461 atgcagttct atctctgcgg cccggttggc ttcatgcagt ttgccgcgaa acagttagtg
    32521 gatctgggcg tgaagcagga aaacattcat tacgaatgct ttggtccgca taaggtgctg
    32581 tgatttgatg ttgccggatg gaaacatccg gcaacccttg acgcggttta aattgccgcg
    32641 tcgtcctctt caccggtacg gatgcgaatg acccgtgcca cgtcgaagac gaagatttta
    32701 ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg tatcaacaca ggtatcgaca
    32761 atgtcgtccg gtacgacaat ctcaattttc actttcggca gaaaatccac catatactcc
    32821 gcgccgcggt acagctcggt atggcctttc tggcggccaa aacctttcac ctcagtgacc
    32881 gtcatgccgg taatgccgac ttcagccagt gcttcgcgaa catcgtccag cttgaagggt
    32941 ttaataatcg catcaatctt tttcatgcta ttccttgaaa aggtcgcctg tcttttgatc
    33001 tgctaaacgt aacacataac gccaattcat tccttgaaat cgtttgcatc cagctcgtgt
    33061 cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg ccattctcgc cgcgtgggtg
    33121 acgttgcctt tggtaatttg cagcagctta cgcaaatagt tgagttcaaa ctgattacgt
    33181 gcctcaacaa aggttggcag cgccgtgttt tcgccctcca gcgcctgctc tactaatgca
    33241 tcgctaatca ccggagatga ggtcagcgcc acgcactgtt caatcacgtt gaccaactgg
    33301 cgtacattac ccggccagct agcggtcatc aggcgtttca tcgcatcggt ggagaacgcg
    33361 cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca ggtgatttgc cagtagcgga
    33421 atgtcttctg tgcgctctgc cagagccgga attttcaggc tgacaacgtt gaggcggtaa
    33481 tagagatctt cacggaattc cccgcgcgcc atcgcttttg gcaggtcgcg gtgagtggcg
    33541 gaaataatcc gcacgtcgat atcaatatcg cggttactgc ccagcgggcg cactttacgc
    33601 tcctgcaaca cgcgcagcag tttaacctgc aacggtgcgg gcatatcgcc aatctcatcg
    33661 agaaatagcg taccgccttc cgccgcctgg aataaacctt cgcgattgct gacagcgcca
    33721 gtaaacgcgc cacgcgcatg accaaacagc tccgactcca gcaattgctc gggcaatgca
    33781 ccacagttaa tagcaataaa tggtttgcta ttgcgcgggc tagcgttgtg gatagcctgg
    33841 gcgaaaatct ctttcccggt gccgctctga ccgttaatca aaacgctaac gtctgattgc
    33901 gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg ggctgcgggt gacaatcgcc
    33961 tcgcgccagc gttcatcggt ggctggcgcg gattgttcca gcgcatcgtc aattgcctga
    34021 tatagcgcgt ctttatcgac aggcttggtg aggaaactga acactccctg ctgtgttgca
    34081 gcaacggcat cgggaataga accatgtgcg gtaagaataa ttaccggcat tcccggctgc
    34141 actttctgga tttcagcaaa cagctgcata ccatccattt catccatccg caggtcgctg
    34201 ataactaaat ctactttttc gcgattcagt acccgtaatc cttcagcacc actttccgcc
    34261 gtgaccacgc tgtagccttc gctggtcagg cgcaggccaa gcagtttcag caatcccgga
    34321 tcgtcatcga ccaataataa atgcgcaggt ttatggctca tcaggagtga cctcatgggt
    34381 ggatggcgcg ggcttttcac tctcgtgcgg cgtatccggc gagaaatttc cagccggttt
    34441 gcgggtcgag agctggcgtt caatatcagt caggttttcc agcttgcggg tggtgagttc
    34501 cagttgctgc tgtagaacgt gatgttgctg gcgcaaagta tccagctcgc tgtcgctgga
    34561 ctgctggagt ttgctgtagc gttggcgctc ttccgccagt tgcagttgca gcgcctgacc
    34621 gtcgcgccaa agttgataca gagggcgaac ctgtgccggg atttcggtac ttagcgcatc
    34681 aatgcgcgcg accagctggc ggcgctcata cggcgtaatt ttggcatcgg cgagcaaaat
    34741 cccttgttta aaggtatttt gccagctgcc gtcgttatat tggcgagctt gctgacgcga
    34801 ctgcgcaggc attaaacgat cagcacaatc catcgcccgc agccagtaaa gcggattggt
    34861 ttcggttgat ttaccttgca gcgcccagat gtcgctacat tcagtagaaa gatagtcagc
    34921 cagttgataa accggaattt tttcttctgc tggcgtatta atggctggct tattgtgact
    34981 ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc agccacagcc gtcggggcaa
    35041 taatcgttga aaaatgtgtc gcatattcac cagacttaat gcctatccca gtgggcgtaa
    35101 ttgttgcagg cagtttggac atggacagcg cggagaaacc ggagcgtaca cgtcgtacgt
    35161 gaggatttcg agcactgccc ggggccgaac tggcaaataa aatagcctga tgggataggc
    35221 tgttagcatt atttcgtgtt tttcgacgac ggcaattcaa tgcggaaaca aacgtcttgc
    35281 ccgctctcgt cgaccagata cagttcccct tgcatacggc gaatacaatc cctggcaatg
    35341 cttaatccca gaccgctgcc cttcaccgcc ccttttcgct ggtggcttcc ttgaaaaaag
    35401 ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggtg tgcctgtatt gatgacatca
    35461 atataaaccc gcgcaccatg taagctgctg cgaaggcaaa tgttaccgga ttcagccccg
    35521 tagtgcaccg cattggagta aagattatcc agtacgctca tcagcagcat tggctccgcc
    35581 aggcaagctg ttgctttgag atcgacgtcg gtatgcatca ttttagcccg tgcgggcagg
    35641 ctatgagcag aaaccaccat ttccaccagt ggcgctaact caacattctc cagttccacc
    35701 gcaccgtccg cctgtttacg gttgtaatca agcagttgtt cgatcaactt ttgcaaattg
    35761 cggctgctgc tatcaagaat gctcaccacc tctttttgct ctggcgtaag cggcccaaca
    35821 acctggtcag ccagtaattc agtgccctca cgcatactcg ccagcggcgt ttttaattca
    35881 tgagataaat gtcttaagaa ttgatgacgt tgggattcca gccatgacag gcgctcactt
    35941 aaccaaagaa tacgttgccc aaccgagcgt aactcactcg gtccactgaa cgaaacgcta
    36001 ttgcccagag aacgcccttc tcccaaccgg ttgatcatcc tctcgatatt ttttaccgga
    36061 ccgataatca tccgcgtgaa aagcagtacc atcaccagac tcaccagaaa tagcaccagc
    36121 gattgccaac caaaatattg cccacgttcg gcgatttcac gctgaagttg ctgcccacga
    36181 gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat tggcactggc aaaggcttcc
    36241 agacgcgcgg cggcggcggc atcgggaccg ctgttgttac actgaagttg agccagattg
    36301 ttcaagtcct gacgcaatgc ctggtaaagt ttatcgtctg gcagtacgcc tgcgtgggca
    36361 tcgagcattt cgctataacg cttgcgctgg ctttgataaa ccttcgcaag cgtagggtcg
    36421 tccagcacgc aatactgacg gtagctgcgc tccatttcca gtgccgcgtt ggtcatcgct
    36481 tcactgcgcc gggcatcaac aagcgtagtg cggttaacca gcgccgcctg atcgctaagc
    36541 gcattcaggc tttgccaggc ttgccatgcc agcaccaaca ggggtagcag aatcagcaaa
    36601 aatgccagca ttaccagttg tcgtaatgag cggggaaaaa cgggccagcg tttcaaggtg
    36661 ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg ggaagttcag atacaacaaa
    36721 gccgggaatt acccggcttt gttatggaat aaggcggtgc ctaactcgac gtttcgcccg
    36781 atggttgata tagctacgct gatatcagaa gttggacggc aggcaccttg ttgtgcgtca
    36841 ttcgaatttt atgtagcacg tcccgaaggg gctgacataa gtcggtgaat gagccactgg
    36901 ttactattat gcagtaactg tgccaataaa gaaaatagtt tggtaacgta ctgattatac
    36961 gctgctttga gggatttatt gcttcttcgc tatttgaatg taaatcagcc actgtgtcgc
    37021 taaaaagaga
//
LOCUS       DAOTRK010000029        36907 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.029, whole genome shotgun sequence.
ACCESSION   DAOTRK010000029 DAOTRK010000000
VERSION     DAOTRK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.029"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            181..909
                     /gene="mpaA"
                     /locus_tag="QIE41_001462"
     CDS             181..909
                     /gene="mpaA"
                     /locus_tag="QIE41_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDS3530364.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(884..1849)
                     /gene="ycjG"
                     /locus_tag="QIE41_001463"
     CDS             complement(884..1849)
                     /gene="ycjG"
                     /locus_tag="QIE41_001463"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDS3530365.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATESRAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1968..2474
                     /gene="tpx"
                     /locus_tag="QIE41_001464"
     CDS             1968..2474
                     /gene="tpx"
                     /locus_tag="QIE41_001464"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDS3530366.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2518..4059)
                     /gene="tyrR"
                     /locus_tag="QIE41_001465"
     CDS             complement(2518..4059)
                     /gene="tyrR"
                     /locus_tag="QIE41_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDS3530367.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDATTV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4207..5268)
                     /locus_tag="QIE41_001466"
     CDS             complement(4207..5268)
                     /locus_tag="QIE41_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDS3530368.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVIDAALRPKRSLWRKMVIGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     DVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5265..6662)
                     /locus_tag="QIE41_001467"
     CDS             complement(5265..6662)
                     /locus_tag="QIE41_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDS3530369.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            complement(6781..7095)
                     /gene="pspE"
                     /locus_tag="QIE41_001468"
     CDS             complement(6781..7095)
                     /gene="pspE"
                     /locus_tag="QIE41_001468"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDS3530370.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(7170..7391)
                     /gene="pspD"
                     /locus_tag="QIE41_001469"
     CDS             complement(7170..7391)
                     /gene="pspD"
                     /locus_tag="QIE41_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDS3530371.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(7400..7759)
                     /gene="pspC"
                     /locus_tag="QIE41_001470"
     CDS             complement(7400..7759)
                     /gene="pspC"
                     /locus_tag="QIE41_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDS3530372.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(7759..7983)
                     /gene="pspB"
                     /locus_tag="QIE41_001471"
     CDS             complement(7759..7983)
                     /gene="pspB"
                     /locus_tag="QIE41_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDS3530373.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(8037..8705)
                     /gene="pspA"
                     /locus_tag="QIE41_001472"
     CDS             complement(8037..8705)
                     /gene="pspA"
                     /locus_tag="QIE41_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDS3530374.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDNGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            8872..9849
                     /gene="pspF"
                     /locus_tag="QIE41_001473"
     CDS             8872..9849
                     /gene="pspF"
                     /locus_tag="QIE41_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDS3530375.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(9969..11234)
                     /gene="puuE"
                     /locus_tag="QIE41_001474"
     CDS             complement(9969..11234)
                     /gene="puuE"
                     /locus_tag="QIE41_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDS3530376.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDDHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPSIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALND"
     gene            complement(11272..12552)
                     /gene="puuB"
                     /locus_tag="QIE41_001475"
     CDS             complement(11272..12552)
                     /gene="puuB"
                     /locus_tag="QIE41_001475"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDS3530377.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(12554..14041)
                     /gene="puuC"
                     /locus_tag="QIE41_001476"
     CDS             complement(12554..14041)
                     /gene="puuC"
                     /locus_tag="QIE41_001476"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDS3530378.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADFMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYTEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLDLLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(14191..14748)
                     /gene="puuR"
                     /locus_tag="QIE41_001477"
     CDS             complement(14191..14748)
                     /gene="puuR"
                     /locus_tag="QIE41_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDS3530379.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(14775..15539)
                     /gene="puuD"
                     /locus_tag="QIE41_001478"
     CDS             complement(14775..15539)
                     /gene="puuD"
                     /locus_tag="QIE41_001478"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDS3530380.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVL
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            15751..17169
                     /gene="puuA"
                     /locus_tag="QIE41_001479"
     CDS             15751..17169
                     /gene="puuA"
                     /locus_tag="QIE41_001479"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDS3530381.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            17318..17482
                     /locus_tag="QIE41_001480"
     CDS             17318..17482
                     /locus_tag="QIE41_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530382.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            17472..18857
                     /gene="puuP"
                     /locus_tag="QIE41_001481"
     CDS             17472..18857
                     /gene="puuP"
                     /locus_tag="QIE41_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDS3530383.1"
                     /translation="MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRAPVSET"
     gene            18991..19236
                     /locus_tag="QIE41_001482"
     CDS             18991..19236
                     /locus_tag="QIE41_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDS3530384.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            19549..21192
                     /gene="sapA"
                     /locus_tag="QIE41_001483"
     CDS             19549..21192
                     /gene="sapA"
                     /locus_tag="QIE41_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDS3530385.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKPDEVKKP"
     gene            21189..22154
                     /gene="sapB"
                     /locus_tag="QIE41_001484"
     CDS             21189..22154
                     /gene="sapB"
                     /locus_tag="QIE41_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDS3530386.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITDFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            22141..23031
                     /gene="sapC"
                     /locus_tag="QIE41_001485"
     CDS             22141..23031
                     /gene="sapC"
                     /locus_tag="QIE41_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDS3530387.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLILGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            23031..24023
                     /gene="sapD"
                     /locus_tag="QIE41_001486"
     CDS             23031..24023
                     /gene="sapD"
                     /locus_tag="QIE41_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDS3530388.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            24025..24831
                     /gene="sapF"
                     /locus_tag="QIE41_001487"
     CDS             24025..24831
                     /gene="sapF"
                     /locus_tag="QIE41_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDS3530389.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(24880..26043)
                     /locus_tag="QIE41_001488"
     CDS             complement(24880..26043)
                     /locus_tag="QIE41_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDS3530390.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSGVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(26052..27425)
                     /locus_tag="QIE41_001489"
     CDS             complement(26052..27425)
                     /locus_tag="QIE41_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDS3530391.1"
                     /translation="MLRRSLIFLVLLSAGCVSLEPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTHVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSEALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQALLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(27429..30536)
                     /locus_tag="QIE41_001490"
     CDS             complement(27429..30536)
                     /locus_tag="QIE41_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDS3530392.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSTPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(30536..31657)
                     /locus_tag="QIE41_001491"
     CDS             complement(30536..31657)
                     /locus_tag="QIE41_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDS3530393.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTVRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(31806..32372)
                     /locus_tag="QIE41_001492"
     CDS             complement(31806..32372)
                     /locus_tag="QIE41_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDS3530394.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            32850..33638
                     /gene="fabI"
                     /locus_tag="QIE41_001493"
     CDS             32850..33638
                     /gene="fabI"
                     /locus_tag="QIE41_001493"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDS3530395.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELAKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            33782..34909
                     /locus_tag="QIE41_001494"
     CDS             33782..34909
                     /locus_tag="QIE41_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDS3530396.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTTELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALICARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            34977..>36907
                     /gene="rnb"
                     /locus_tag="QIE41_001495"
     CDS             34977..>36907
                     /gene="rnb"
                     /locus_tag="QIE41_001495"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDS3530397.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHIDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT         8609 a        10027 c         9311 g         8960 t
ORIGIN      
        1 taaaatacgc catgtatata acattacgac aattctaata tctgcacatc aatgaaaatg
       61 caaacaactt ccggcaaaaa agtgatacaa gatcgatgac atagcgttga agaaaaggat
      121 ttgataatcc gcaaaatttg cacaacactg cctgtaatac ttcctgaacc agagacgatc
      181 atgaccgtaa ctcgcccacg cgccgaacgc ggcgcatttc cgcccggaac agaacattac
      241 ggacgttcat tattgggcgc gccgttaatc tggtttcctg cccctgcagc cagccgcgaa
      301 agtggtttga ttctggctgg cactcacggt gatgaaaact cttcgatcgt cacgctctcc
      361 tgtgctctgc ggacgttgac accttctttg cgccgccatc atgtggtgct gtgtgtgaat
      421 cctgacggct gccagttagg gttacgggcc aatgctaatg gtgtggattt aaacagaaac
      481 tttccggcgg cgaattggaa ggaaggtgaa accgtttatc gctggaacag cgccgctgaa
      541 gaacgtgatg tcgtattgct gacaggtgac aaacctggct ctgaacctga aacccaggcg
      601 ctgtgccagc ttatacaccg cattcaaccc gcctgggtgg tctcattcca cgatccgctg
      661 gcctgtattg aagatcccag acacagtgaa ttaggcgaat ggctggccca ggcgtttgaa
      721 ttgcctctgg taaccagcgt cggttatgaa acacctggct ctttcggcag ttggtgtgca
      781 gatctcaact tgcattgcat caccgcagag tttccgccca tctcctccga cgaagccagc
      841 gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc atcctaaaga tgcaattcgc
      901 ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag ccaggtcggt ccgtcaagat
      961 cggcgaaact gacctgcggc accagcggta aagcggcgct aatggcacga gaggtacaca
     1021 acatgcagcc cagcatcaga ctaaaacctt gtgcacgtga ttcagtcgcc agcgccagcg
     1081 cttccgtcag gccacccgtt ttatcgagct taatgttaac catctcatag cgccctttca
     1141 gcgccttcag attgctacga gtatgacaac tttcatctgc gcaaatcggt aacggatgaa
     1201 taaaattctc caacgccgca tcgtcctgcg caggaagcgg ttgttcaagc atcgcaacgc
     1261 ctaaatccgc caatagctgg caacgcgccg ccaacccttc tgcacgccag gattcatttg
     1321 catcaacgat cagcgtcgca tcgggcacag cggtgcgaat tgccaccatc cgctcactga
     1381 taagatggtt atccagcttt actttcagta atttcgcgcc tgcctgccag agtgttgatg
     1441 cactattggc catctgatca ggcgtaccga tgactaccgt ctgtgcagtg ataactgtcc
     1501 cgggaagcgt tatgccgatc aaatcagcca gcgattgctg ctgttttcgc gccgcgagat
     1561 cccacaatgc acaatccagc gcattacgtg ctgcgccggc agggagaatt ttttgcaact
     1621 cctcccgtgt cagccctttc tctaattgcg gtacgacgct cataatttgc gccattaccg
     1681 aggcatcact ttctccataa cgcggatacg gcgtgcattc gccggtgcct ttaatacctt
     1741 cttcttccag ttcaaccacc accacgcgcg cttcactgcg acttccccga gcaatcacaa
     1801 acggggtatg taagggccag gcttcctcga ataccttaac ggttctcatc tttactcctt
     1861 gttgtcgcgg tggtctgcgg attaagcgca aatagcgttt gctgtgttat tgacagttag
     1921 cataaactag gtgtgacgtt aactatatgt aaacaggaaa gataatcatg tcacaaaccg
     1981 ttcatttcca gggcaacccg gttacagtcg ccaattccat cccgcaggcg ggtagcaaag
     2041 cgcagacttt tactctcgtg gcaaaagatc tgtctgacgt caccctcggt cagtttgcgg
     2101 gtaaacgcaa agtgctgaac attttcccga gtattgatac cggtgtttgc gccgcatcag
     2161 tacgtaagtt taaccaactg gcaaccgaga tcgacaacac cgttgtgctg tgtatctctg
     2221 ccgatctgcc gttcgcccag tctcgtttct gcggcgcaga aggtctgaac aacgttatca
     2281 ccctctccac tttccgtaac gctgaattcc tgcaagccta cggtgtggca attgctgatg
     2341 gcccactgaa aggtctggca gcgcgtgccg ttgtggttat tgatgaaaat gacaatgtga
     2401 ttttcagcca gctggtggat gaaatcacca ccgagccgga ttacgaagca gctctggctg
     2461 tactgaaagc ataagttaat atgcctgatg gtgttgcacc atcaggcata ttcgcgctta
     2521 ctcttcgttc ttcttctgac tcagaccata ttcccgcaac ttattggcaa tcgcggtatg
     2581 cgaaacgccc aggcgttttg ccagtttgcg cgtgctggga taattgcgat aaagctgggt
     2641 taataccgag cgttcaaaac ggctggtgat ttcgtccagc gaaccttcca tcgcatcttc
     2701 gcccacggct accgttgtgg cgtcataatc cggtaacaaa atatcctgtg gacgcagctc
     2761 ataaccgtcc agttgtgtca gtgcgcgata aatagcgttc tttaactgcc gcacatttcc
     2821 cggccacgca taacgcgtaa gtacagtatt caggtcagcg gccagtttcg gacgcggcac
     2881 gccctgctcg tcggcaaagc gggcgacgaa cagctcggtt aacggcatga tgtcctgcgg
     2941 acagtcacgt agcggcggca gattgagcgt caacacgttc agacgataat agagatcttc
     3001 acggaacacg cctttttgca ccagttcgac cagattcttc tgcgtagcgc aaatcacccg
     3061 cacatcgaca tgcacctcat ggtcttcgcc aacccgacgg aaagtgccat cattaaggaa
     3121 acgcagtaat ttcgcctgca tccgtggtga catctcacct atttcatcca gcagtaccga
     3181 accaccgttc gcctgctcaa agaacccttt cttcccttcc ggagcatgac caaacagttc
     3241 actctcgacc gcatcttccg ggatagaagc acagttcagc gccagatagg gtttgcccgc
     3301 gcgtgggctt gcctgatgac aggcatacgc aaagagatct tttccggtgc cggtatcacc
     3361 ggttatcagc aatggcgcgc ttagcatcgc cagtttctgc gcctgttcga caacatgctt
     3421 cattttcggg ctgacggcga caatttgact aaaggcgctg acgtcctggg cggcgacatt
     3481 ttgcaactga cggcccatac gaatcgttga tcgcaacatc accaccgcac cggtcaggac
     3541 gtgttgatca ttttcatcct gaagataaac aggcgtaatc tccatcagga aattctgtcc
     3601 attaataacg acatgctcgt tatgggaatc ctgcggttcg ctttccagcc agcgtaaaaa
     3661 attaaagccg ttaatcaatt gtgcggcggt atggttgcgc aggcgatcca atttttgccc
     3721 aaaaagctga cagctcgccg ggttcgccat atccactttg cttttcatat cgacagagag
     3781 cacaggttca ggcaatgcct ccagtaacgc gctcaacgcc agatgctcac gctcggaagg
     3841 catccacggg acagtacgca catcggtaac acctgcaata cggcgtattt cggccatcag
     3901 actgctgaaa ctctcaaact ccagttcagc aaaattgagg tagattcgcc caatgggatc
     3961 aatctcaata ccgcgtaaat caatgcctct tagcacgagt agatcgagta attcgcgggt
     4021 cagaccgagt cggtcttcac aaaagacttc cagacgcatg ggaaccttca cctgaaaaaa
     4081 gaacaattaa tatgatgata tgacactatt tggatagcag gaaggtttct gtcaacaatc
     4141 attgacacaa agacggaaaa acgggcactt cggcgtaaag atatccggtc agcacccgat
     4201 attgcattat ttttcgctgg gcgttttgcc tttttgcaga gtttctttca cctgaccgat
     4261 aagctgacga cggaaatccc cgaggcgtgg cttgtcatcg tcaatccacg gcagagggcg
     4321 gcaaagctcc atagctttaa tccccagtcg tgccgtcaga agtcctgcac caatcccctg
     4381 agctgcccgg gtagacaaac gcgcagcgag atcttgcgac atccagtcca tccccacgtc
     4441 gcgcaccagt tcgctggctc cggcgaaagc gatattcagc aataccagct taaacaggcg
     4501 caaacggctg taatatccca gttcaatgcc atacagcgtg gcgatgcgat taatcaaacg
     4561 cagattgcgc caggcgataa acgccatatc caccagcgcc agcgggctga ccgcaatcat
     4621 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
     4681 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
     4741 ggcataccag cgctgcagcg ccggatgcga ctgatcgata cccgcctgct gcgccagttt
     4801 ttcgcagaat gcacggcctt tacccgtgcc gtgactgtgc aacaaatcgc gcgcttcgtc
     4861 gcgttcatgg gcgcgctgtc gcaagcgcca taagcgccgc cactctgtta ccacagaacc
     4921 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcca cccagtcctg
     4981 ggtttgccag gcgttcattg tccactgcac gccctgcccg acaacgcttg cgccaaacag
     5041 agccaacccg cctatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcgtc
     5101 gatcaccgct tcaacttgcc cctcttcttc ctgcgcttcg tcgagcgtgg ccggggcaaa
     5161 attttgcgcc tgattttcgt caaaggtctg ttgcgccctg aattttggat tctgatcgac
     5221 ctccagtgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     5281 cctattaaaa attccagcgc agcatcaaga cgaatatgcg gtagcggttt gtcgacatcc
     5341 atcacctgcg gacgaaacgc ctcaaactgg aagccttgct tatcccagaa cgcctgacca
     5401 ggcaaacgtg cgggaacttc gccaggataa acagtgagcg gtgcgccatc actgagtcga
     5461 ttaccgcgca gcgccgggat tttctcaccg ttaacatcaa taatgccgct ggtggtcgcc
     5521 tgaactgacg ccagccccag gcagtccatg ctgatccctt cgaacgccgc attttgccag
     5581 gcatcctgaa ttagttgttg cagtaatgaa accatattgg cgtgctgatc gatggtcaca
     5641 tggtctgctt tagtcgcagc aaacaatagc ttatcgataa ccggcgaaaa caaacgccgg
     5701 aacagggttc gctgcccgta gtggaaactt tgcatcagct gcgtcagcgc cagacgcata
     5761 tcattaaatg cctgtggccc actgttgaga ggctgcaggc aatccaccag cacaatctgg
     5821 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcaataataa
     5881 ttaaagcgct cgcgcagcat tccggcattg gtatgcttat cggcctgcgc cagtttggac
     5941 tcgccccagg catcgacatc cggccacggg aagaattgca gcgcgggtgc tcctgccata
     6001 tctcccggca agacaaagcg cccaggctga ataaagtgca acccctgctg tttgcagtgg
     6061 tggagatact cggtccaggc ggcggcaatg tctgccagcc ggttttcgtc ggcaggcgct
     6121 agcgggtcca gcccttcgca catcattcgc catttcgccg accattcgcc gcgctgacca
     6181 ttgagtaagc ccgtcatctg gcgcgaccag cttaaatagt cctgcgccag catcggcagg
     6241 tcgagcaacc attcgccggg gtaatccaca atttccagat acagcgtgga ggtgtcctta
     6301 aagtggcgta gcagcgaatc gttcgattta aaacgtaacg ccaggcgaat ttcactgacg
     6361 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccaa cccttcatca
     6421 taggtgaagc gcggaatgcc aaagtcacgc tggggaatgc gttttacgcc cagcaggcgc
     6481 tcttcacgca ccgcacttaa aagcggcaaa cgtgctccgg catgaatatt gagcaactga
     6541 ttaaccatcg cggtgataaa cgctgttttg ccgctgcggc taagtccggt tacagcgagg
     6601 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     6661 attgctgtcc tgtgccagaa agctgtagaa aggattagat actggagata ataatgtggg
     6721 ttaattttga tttatcaaat gagtaagcgg cgttgtttac accgctcaat aagtaatttg
     6781 ttaacctttg accttcggca ttgcgatgtc tttcaggccg ccggcgttct caacgtgggt
     6841 atatcccatc tcgctaagga tctcttttgc ctgccctgac tggcgtccgg cattgcaata
     6901 cactttcacg gtgtcgtttt tatccggaac ggcggtggca atgcgctctt tcacttcctt
     6961 caggggaata ttgatggctc cctgaacgtg ctcttgctga tactgctctg gaacacgaac
     7021 atcgatccag tgttcagcgg cgaaaacggg cagtgaaaac accagtgtca gagcaagtaa
     7081 gcctttttta aacatgtaag cgtccttcta tggatgaaaa ttgccatcca taatatgcct
     7141 tagctttaac aatttttgcg aaactcccct caccttttat aacgctgcgc cagtttattg
     7201 gcagcccgac ttaacagcgg ttccagcgcc acggccagca acattttcag tggtcggcga
     7261 gcaactgatt ttatcgccca gccagctaca cccgccgggc catagcgcag tgcggtaaga
     7321 agtaccagct tgcctgctaa tttgaaacca ggcttcacct tttgcccggc ctgttgccag
     7381 cgagtattca taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg
     7441 aagtgacata acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga
     7501 cttcatccag caattcactg ctggaaggta gctgctcacc aaaggccatg ttgtccggca
     7561 ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga
     7621 agaaaatcga cagcaccacc aggatacgca ccagttttac tggtacatca aaatagttgg
     7681 caatcccggc gcagacgcca cggaccatgc cctgctgtgg aatacgccat aattttttat
     7741 tgagattaat gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaatgga
     7801 ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg
     7861 ctgctgctca ctttgcgaca attcactgcg accagaacga ttgctgtaat gcagccataa
     7921 ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgct
     7981 cataaggact ccttagacga tgagcggcgg cgcgttcaga cgccgcctgg atgttattat
     8041 tgattgtctt gcttcatttt ggcttttaat tgtgccagtt gctcgctgat tgcatcatcg
     8101 gctttcagtt cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct
     8161 gcttccgctt ccatctggtc aattcgacgt tcgaaagatt cgaaacgagc cattgcttca
     8221 tccagtttgc cattgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatgg
     8281 cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcgccg
     8341 atctctttct tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg ttccaggctc
     8401 ttaatcagat cggtcagttt ctgtttttca attaacgccg cacgcgccag atcctctctc
     8461 tctttcagca gcgccagttc ggctttttcc tgccattcaa cttcacgcgc cgacgcttgt
     8521 tcaatacggc gagtcagctg tttcttttct gccagcgcac gcgccgaagt agaacgcact
     8581 tcaaccaatg tatcttccat ctcctggatc atcaaacgca ccagtttctg tggatcttcc
     8641 gctttctcta acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata
     8701 cccataatgt tgtcctcttg atttctgcgt tcaggattgt cctgctgaac tattaataca
     8761 atttgcgtgc caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc
     8821 aacatgccag gatgagttag cgaattacac taacaagtgg cgaatttcat catggcagaa
     8881 tacaaagata atttactcgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg
     8941 catctcgcac cgctggacaa accggtgctc atcatcggcg agcgcggcac aggtaaagag
     9001 ctgattgcca gccgtctgca ttatctctcc tcccgttggc aggggccgtt tatttccctt
     9061 aactgcgcgg cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggc
     9121 gcgtttaccg gtgcgcaaaa acgtcatcca gggagatttg aacgtgccga cggcggtacg
     9181 ctatttcttg atgaactcgc tacggcaccg atgatggtgc aggagaaatt attgcgcgtg
     9241 attgagtacg gtgaactgga gcgcgttggc ggcagccagc cattgcaggt gaatgtgcgg
     9301 ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca atgaaggtac ttttcgcgct
     9361 gacctgctcg accggctggc tttcgatgta gtgcaactgc cgcccctgcg cgagcgcgaa
     9421 agcgacatta tgctgatggc agaacacttt gccattcaga tgtgtcggga aatcaagctg
     9481 cctctgttcc cggggtttac ggagcgcgcc agagaaacat tgctgaatta tcgctggccg
     9541 ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag tatatcgcca cggcaccagc
     9601 gattatccgc ttgatgacat tattattgat ccctttaaac ggcgtccgcc tgaagacgct
     9661 atcgccgttt cagaaaccac ctcgcttcca acactgccgc tggatttacg tgagtttcag
     9721 atgcagcagg aaaaagagtt gctgcaactc agtttgcaac aagggaaata taaccagaag
     9781 cgcgcagctg aattgctggg gttaacctat catcagttcc gcgcgttgtt gaaaaagcac
     9841 cagatttagc gaagttgggc aatacaactt gccggatgcg gcgtgaatgc tttgtccggc
     9901 atacataatc aatacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
     9961 ttgggctatt aatcgttcag cgcatcctgc aatattttca ttgccgcatc gaattgcgca
    10021 tccgggatgg tcagcggata caggaaacga atcacgttgc cgtatgcgcc acaggtcagc
    10081 agaagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
    10141 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatag accccagacc gcgtaccgcc
    10201 gcgatggacg gaacgctttc tttggcatca atcagcgtgt ttgtcagacg ctggcccagt
    10261 tgattcgcgc gttcgcagag tgattctttg tcgataatgt tgagcaccgc gtgcgcggca
    10321 gccaccgcca gcgggttccc ggcgtaggta ccaccaagcc cgcccggcgc gggtgcgtcc
    10381 ataatgttgg cattaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
    10441 atcgtcatta aatccggctt atcggcgtag tgatccatgg caaacagttt accggtacgc
    10501 gcaaagccgc tttgcacttc atcggcaatc atcacaatgc cgtgatcgtc gcacaggcgg
    10561 cgaatggcgg caaccagctc ttttggtgca acgttgaaac cgccttcgcc ctgcaccggt
    10621 tcgaaaataa tcgccgccac ctgcttcgct tcaatgtccg atttaaacaa gcgttcgatg
    10681 gcgtcgaggg agtcctgcgt tgaaatgccg tgtaaatctg acggataagg tacgtgatat
    10741 accgaaccgg ggaacgggcc gaagccgatt ttgtacggcg cgacctttcc ggtcagcgcc
    10801 atagtcatat atgtacgacc gtggaagccg ccgctaaacg caatcacgcc agggcgtccg
    10861 gtatgggcgc gggcgatttt caccgcgttc tcaaccgctt ccgcaccggt ggtgaagaac
    10921 gcagttttag cctgcccgct caccggggca agggcgttaa ttttctccgc cagcgtgacg
    10981 tagctttcgt acggcacaat ctgatacgcc gtgtgggtaa actgttgcaa ttgctgctcc
    11041 accgccgcga ccagatcagg gtggcgatgc ccggtattca gcaccgcaat gcctgcggcg
    11101 aaatcgatat attcgttgcc ctctacatcc ttcagcgtag cgttttctgc cgactgggcg
    11161 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
    11221 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
    11281 cagacgatcg cgcaggctgt aataagccgc gcccatcgcg gtaaacggaa cgcgcagcgt
    11341 gcgcccgccg gggaacgggt aatgcggcag attggcgaag gcgtcgaagc gttcggcgtc
    11401 accgcgcagc agttcagcaa tcaaacgccc ggcaagatgg gtacaggtga cgccgtggcc
    11461 gctgtaaccc tgcatgtaat agatgttggt atcaaggcga ccgaactgcg gcatgcgcga
    11521 caaggtcagc aggaagttgc ccgtccagcg gtaatcaatt ttcacgccct tcagctgcgg
    11581 gaaggttttc agcagtttcg gcaccacaag gcgctcaacg tcatccgggt cgcgcgcgcc
    11641 gtagaccaca ccgccgccgt acagcaggcg gttatcggcg gtaagacggt agtaatcaag
    11701 cagatagtta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
    11761 cgacagccgt tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
    11821 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
    11881 ctgaccattg gcagtccgca ccacggctgg cgtggtgtgc tggatctgcg tcacggcgga
    11941 aagctcatac acgcgccctc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
    12001 tagcgggtga atatgcccac cgctgtgatc cagaagcgca ccagtgtagc gatcgctggc
    12061 gacctcgcgg cgaatagcgt tggcgtccag cagctccagc tgtttattgc cgtagcgttc
    12121 ccagttctct ttctgctctt ccagagtcgc gagctgttta tcattcatcg ccacaaacag
    12181 gccgccgggg cggtagtcgc aatcaatctg ataacgtttg atacgttcgc ggatgatctc
    12241 gccgccttcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
    12301 aatgacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccgttgc gtccgcttgc
    12361 gccgaagccg atgcgtgagg cttcaagaac caccacgtca aagcccgctt ccgccagatg
    12421 cagcgcggag gaaagcccgg tatagccgcc gccgaccacg caaacatcgc aggaaatcga
    12481 ctcattcagc gtgtcgaatg gtgcgtattt attcgcactg gcggcgtaat aactgctggt
    12541 atgttcggtc atttcaggcc tccaggctta tccagatagt tttcagttcg gtgaattttt
    12601 caagggcgtg cagggatttg tcgcgaccat tgccgctctg cttatagccg ccaaacggca
    12661 cggtcatatc gccgtcgttg tagttattga caaagacgga accggctttc aggcgacggc
    12721 tcatgcggtg cgcgcgggag aggtcgcgcg tccatactgc cgcgccaagt ccgtactggc
    12781 tgtcgttagc aagctgtagc gcctgttctt ctgatgtgaa acgcgtgacc accagcactg
    12841 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc tacatccaca aagatcgtcg
    12901 ggccgatggc ggcagccagc tcggcgttac ggccatccag cagcagttgc cctttgcttt
    12961 cgccttcacg aataaagcta tggaccgagt cggcgtgggc gcagtcgatt aaggtgccca
    13021 tggtggttgc gggatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
    13081 ggtctaagaa ttcatcggcg atgctctctt ccagcaacag gcgcgttccg gcgatacaca
    13141 cctgcccctg gttgtagaag atgcctgctg cggtcgcgct cgccgctttt tgcaaatccg
    13201 gacagtcagc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
    13261 tgttgctgtc gcctgcatct ttcagcagct gtttcccggt acgggtcgaa ccggtaaagg
    13321 cgatggcatc gatatcgtta tgacgcgaca gcgcctgccc cgcttcatga ccaaaacccg
    13381 tcaccacgtt caacacgcca tccggcagac ctgcttcttt cgccagcccg gcgagacgaa
    13441 tcgcactgag cggtgatttt tcagacggtt ttaggatcac gctgtttccc gccgccagcg
    13501 ccgggccgag tttccagcaa gtcagcagca gcgggaagtt ccacggcacg atggcggcaa
    13561 tcacgccgac cggttcacgc acgatcatcg ccagctcatg gctactggtg gtcgccactt
    13621 cgccatacac tttgtcgata gcctcggtgt accagcgaat ggcgcgcgcc gcgccgggaa
    13681 tatcatcacg caggctgtga cgaatcggtt tgccggtgtc gagcgtttcc agcagcgcca
    13741 gctcttcggc gttggcttcc ataaaatcgg cgagtttatt cagtaccgct ttacgtttag
    13801 ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc gcgtgctgcg ctcaccgcac
    13861 ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag cggtgcctgg gtgaccggat
    13921 caacggtttc aaaagtttca ttttccgccg cagcagtata ttcaccgtta ataaataagc
    13981 gggtttcaat ggcgagactt aacgctttat cctgccagta agccagatga tgaaaattca
    14041 ttatgactcc tgtttcacgt ctatcagata tatgcagttt gtgaacgtca gggcgcagag
    14101 atccccgggc cgtatgcccg tttatttctg gcgacattct ttatagattg ccgggcgcat
    14161 tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat
    14221 aattcggcaa atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc
    14281 ataactttgc cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc
    14341 ttccagtaca gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc
    14401 cggctggtaa gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag
    14461 cttcattgac acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg
    14521 cggctcatca ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt
    14581 cagcagcttt tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat
    14641 agcactgtga gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg
    14701 gcggatttcc gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc
    14761 ttaaactgta gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata
    14821 aagccctcga acaatatacg cgaaagcgcg tactcgctac tgttccattc cgggtgccac
    14881 tgtacgccca gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc
    14941 gagcgagctt caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgcagagag
    15001 tttacccaaa agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc
    15061 agaacttcat gcgacggcgc atattgctgc tccaccggca gttcgggatc ttcccgatgt
    15121 tccagcaatt caggctgttc gcacagcttg cgatgcaacg aaccaccggt tgccaccacc
    15181 agttcctgta aaccccggca gatggcgaaa atggggatgc gcctttcgag tgcggcatta
    15241 attaacgcca tgctcagaag atcacgcccg ggatcggcgt caggctcatc gccgttttca
    15301 ccatataggt gcggctgcac attgctggga ctaccaggaa gataaatacc atcgagtttc
    15361 ggcaaaagtt gttcaagtaa tgacggttcc gccagcgcat gtggcagcgc aattggcaag
    15421 ccgcctgcgt ggatgatggc attcaggtac ttttcttgca gagtctgggt cgcatgaccc
    15481 ttaagcctgt tcctgcacat tacgacaccg ataaccggat tgttcattat attttccatg
    15541 ctcaatctca caaagtggac taaattatcg ccattactgc gctttaaatg acctttatgt
    15601 tcaatatttt ttcaatctag cagtgggttt tcatttttgc aaactcaatt taacatttga
    15661 caaacattta gtttgcatac agattcgaat ggtggtcatt atattttacg ctttgataac
    15721 gagcggaaaa caaaccaaag gcgaagaatc atggaaacca atatcgttga agtagagaac
    15781 tttgttcagc agtcagaaga gaggcggggt agcgccttta cgcaggaagt gaaacgctac
    15841 ctggagcgct acccgaatac acaatatgtt gatgttttac ttaccgattt gaatggttgc
    15901 ttccgtggaa aacgtattcc tgtttcaagc cttaagaagc tcgagaaagg gtgttatttc
    15961 ccggcctcgg tatttgcaat ggatattctg ggtaatgtag tagaagaagc gggcctgggt
    16021 caggaaatgg gcgagccgga tcgtacctgt gttcctgttc tcggttcctt aaccccttct
    16081 gccgccgatc cagagtttat aggtcagatg ctcctgacca tggtcgatga agatggcgct
    16141 ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt ggcagcagct gcgccagcgc
    16201 ggattgttcc cggtcgtagc ggtagagctg gagttctatt tactggatcg ccagcgcgac
    16261 gctgaagggt atctgcaacc gccctgcgcg cccggcaccg atgaccgcaa tacacaaagc
    16321 caggtttact ccgttgataa cctcaaccac ttcgccgacg tgctcaatga tattgatgag
    16381 ctggcgcagt tacagctgat tccggcagat ggcgcggtcg ccgaggcctc gccaggtcag
    16441 tttgaaatca atctttacca taccgataac gtgctggaag cttgcgatga tgcgctggca
    16501 ctaaaacgcc tggtgcgtct gatggcagaa aagcataaga tgcacgccac ttttatggcg
    16561 aagccgtatg aagagcacgc gggcagcggg atgcatatcc atatcagtat gcaaaataac
    16621 cgtggcgaga acgtgctttc tgacgcggaa ggcgaagatt cgccgctgct gaaaaagatg
    16681 ctcgccggga tgattgacct gatgccgtca tcaatggcgt tgctggcacc aaacgtgaac
    16741 tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc aggcgtcgtg gggccataac
    16801 aaccgcaccg tcgccctgcg tattccctgc ggcgatcgcc ataatcaccg cgtggaatat
    16861 cgcgtggcgg gtgccgatgc caacccgtat ctggtgatgg cggcgatttt tgccggtatt
    16921 ttgcatggcc ttgataacga actgccatta caggaagaag tcgaaggcaa cgggctggaa
    16981 caggaaggct tacccttccc gattcgccag agcgatgccc tgggtgagtt tatcgagaat
    17041 gatcacctgc gccgctattt aggcgaacgc ttctgccatg tctatcacgc ctgtaaaaat
    17101 gatgagctgt tgcagtttga gcgcctcatc acagaaacag aaattgagtg gatgttgaaa
    17161 aacgcgtaat accccttgcg gggttgtttc atcggcctga gcggagccag gcctgacatt
    17221 tgctggtaat ccagacattt cccggatgtc gcgttatgga gcgcaggcgg caacgggctt
    17281 ccgttaacga ttaagactgt gacgaggaaa tgagatgatg ccgatgatta aatccccgca
    17341 tggcgaggga ggctgcgtat gcgcccctcc tgcgactgac tggactccgc cccctttgct
    17401 tcccttgcta aacaggtttg atttcaggtc gacacgaccg caaaccttat tacgcagggg
    17461 aggcagcaac tatggctatt aattcaccac tgactattgc tgcgcaatct ggcaaaaccc
    17521 gtctgcgaaa atcactgaaa ttgtggcagg tggtgatgat gggtctggcc tatctcacgc
    17581 cgatgaccgt atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg
    17641 cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg
    17701 ttcgccagtt tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc
    17761 acgtcggatt tatggtcggc tggtcatcac tgctggatta tctctttttg ccaatgatca
    17821 acgttttgtt ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt
    17881 gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct gaagagcgtc aacctggtcg
    17941 ctaacttcaa taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc
    18001 tggtggttca gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt
    18061 ttatcagcga gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct
    18121 ccttcctcgg tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg
    18181 tgatcccgaa agccatcttc ctgacggcgg tatacggcgg cgttatcttt atcgctgcgt
    18241 cgttctttat gcagctgttc tttcccgata tcagccgctt taaagacccg gacgccgcac
    18301 tgcctgaaat tgcgctatac gtcggcggca agctcttcca gtcgattttc ctctgtacca
    18361 cgtttgtgaa cacgttagcg tctggcctgg cgtcgcacgc cagcgtgtcg cgtctgctgt
    18421 atgtgatggg gcgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccgaaat
    18481 ggcggactcc ggcactgaac gtcattatgg tcgggattgt cgcgttgtca gcgctgttct
    18541 ttgatttagt caccgcgaca gcattgatta acttcggtgc tctggtggca tttaccttcg
    18601 taaatctgtc ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg
    18661 atcacttcca ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg
    18721 ttaatctcga gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat
    18781 atttgtggta tctgatccgc cgctatcgca aagtgccgtt gtatgatggt gacagagcgc
    18841 cggtgagcga aacgtaatag tcggatgcgc ggcgcattat cctgcctgag ggtaatgcac
    18901 cgcgccatta actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta
    18961 tgcttagcgc attaacccca gggggaaata atgaaccacg atatcccgct aaaatatttt
    19021 gatattgccg atgagtacgc gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca
    19081 ccgctggccc tctacttcca gttactgctc acccgtttga tgaacaatga agagatcagc
    19141 gaagaagccc aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag
    19201 atcgcagagt ttttgaatca atggggtaat gagtaattat ttcattgcag acgctcggtc
    19261 agtatcgttg cccggataaa gcgttcacgc cgcatccggc taacaatgac gcgcactgca
    19321 gcgtgcttta atgggtttct cacgatcgta ccgctctgct cctcgctaag ttgttctgtg
    19381 agttatagtt atagagaaat cacgggtacg aatgttgaac agatggcgac gtcacctgtt
    19441 caacgaccaa ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt
    19501 cattaaatat tagtaagata gcctctttac ataaaatcca ttaatattat gcgccaggta
    19561 ttatcgtctc ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa
    19621 tcccccccgc atgctgatat ccgcgatagc ggttttgtct attgcgtcag cgggcaagtc
    19681 aacaccttta acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag
    19741 ttttatgatc gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc
    19801 gaaagctggg aagttctcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt
    19861 ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg
    19921 tttaccttcc agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac
    19981 ttcccctact tcgacagcct gcaatttgcc gataacgtga aaagcgtccg caaactggat
    20041 aatcatacgg ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctggcg
    20101 acccattatg catcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgt
    20161 caggaacaac tcgaccgtca gccggtcggc actggaccgt atcagttgtc ggaataccgc
    20221 gccgggcaat atattcgctt acaacgtcat gatgatttct ggcgtggtaa gccgttgatg
    20281 ccacaagtgg tggtggattt aggctctggc ggtaccggac gtctgtcgaa actcctgacc
    20341 ggggagtgcg acgttctggc ctggcctgct gccagtcagc tatccatttt gcgtgacgac
    20401 ccacgcttgc gtttaacgct gcgtcctggg atgaacgtcg cctatctggc attcaacacc
    20461 gccaaaccgc cgctaaataa tcccgctgtc cgccatgcgc tggcactggc gattaataac
    20521 cagcgtctga tgcaatccat ctattatggt acggctgaaa cggcagcctc tattttaccg
    20581 cgcgcctcgt gggcctatga caacgaggct aaaattactg aatataatcc ggcgaaatcg
    20641 cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtaccaaca
    20701 cgttcgcagg cgtggaaccc cagtccactg aaaactgccg aattgattca ggcggatatg
    20761 gcacaggttg gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcgcgg
    20821 ttgatggata tgagccatga tctgacgtta tccggttggg cgactgacag taacgacccg
    20881 gacagtttct tccgtccgtt actgagctgc gcggcaattc attcgcagac caacctcgcc
    20941 cactggtgcg atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg
    21001 gcggcgcgta ttgaagccta tgacgaagcg cagagtattc tggcgcagga attacccatt
    21061 ctgccgctgg cgtcgtcatt gcgcttgcag gcctatcggt atgatatcaa aggtctggta
    21121 cttagtccgt ttggtaacgc ctcctttgct ggggtgtatc gcgagaaacc ggatgaggtg
    21181 aaaaaaccat gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc
    21241 tgctgacctt cgttggcttc agcttgagct atttcactcc acacgcgccg ttacaaggcg
    21301 cgtcactgtg gaatgcctgg gtgttctggt ttaatggcct gatccactgg gattttggtg
    21361 tttccagcat taacggtcaa ccgattgccg aacagttaaa agaggtcttc ccggcgacga
    21421 tggaactgtg catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga
    21481 ttgccgggat tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg
    21541 gcttttcaat cccggtgttc tggctggcgc ttctgttgac gctgttttgt tcactcacgc
    21601 taggttggtt gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg
    21661 attttgcgtt gattgatgcc tggctttcgg actcaccatg gcgggatgaa atggtcatga
    21721 gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag
    21781 tgatccgact gatgcgtatc agcactatcg aagtgtatga ccagaactat gttaaagcag
    21841 cggcgacccg cggtttgtcg cgctttacta tcttgcgtcg ccacgttctg cataacgcgt
    21901 tacctccggt tattcctcgc ctgggcttac agttttctac catgctgacg ctggcgatga
    21961 tcaccgaaat ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc
    22021 agcaggatta cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg
    22081 ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt
    22141 atgccttacg atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg
    22201 cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct
    22261 gtactgtgta tttttggcgg ctggtttgcg ccttacggca tcgatcagca atttctcggt
    22321 tatcaattat tgccgccgtc atggtcgcgc tatggcgaag tttctttctt ccttgggact
    22381 gacgacctgg gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtaggc
    22441 ggcgcatttg tggtgacgct tgccgcgacg atctgcggcc tgatactggg caccttcgcc
    22501 ggcgcgacgc acggcctacg ctcagcggtg cttaaccata ttctggatac cttactggcg
    22561 atcccttccc tactgctggc aattatcgtt gtagcgtttg ccggaccgag tttgtctcac
    22621 gccatgtttg ccgtctggct ggcgctgctg cctcgtatgg tacgttcgat ttacagcatg
    22681 gtgcatgacg aactggaaaa agagtacgtt atcgctgccc gtctggatgg cgcatcaacg
    22741 ctgaatattc tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc
    22801 acccgcgcac tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc
    22861 gcacaactcc cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat
    22921 gtcgccccgt ggactgtcat gctgccaggt gcggcaatta tgattagcgt gctgttggtt
    22981 aacctccttg gcgacggtgt ccgtcgtgca attattgcgg gggtggaata atgccattac
    23041 tcgatattcg taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg
    23101 accgcgtaag tatgacctta accgaaggtg aaatccgcgg tcttgttggc gaatccggtt
    23161 ccggcaaaag tttgattgcg aaagcaattt gtggggtgaa taaagataac tggcgtgtta
    23221 cagctgatcg tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc
    23281 gcaagctggt tggtcacaac gtgtcgatga ttttccagga accgcagtcg tgtcttgatc
    23341 cttcagaacg agtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc
    23401 gttggtggca gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg
    23461 ggattaaaga tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggcgaat
    23521 gtcagaaagt gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg
    23581 aaccgacaaa ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctc ctgacgcgtc
    23641 tcaaccaaaa cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc
    23701 aatgggcgga taaaattaac gtgctttact gcggtcaaac tgtggaaacc gcgccaagta
    23761 aggagttggt gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag
    23821 acttcggcag cgcgatgccg cataaaagtc gcctcaatac gctgcccggc gctatcccac
    23881 tgctggaaca gttaccgatt gggtgtcgtc tggggccgcg ttgcccgtat gcacaacgag
    23941 aatgcattgt gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc
    24001 cgctgaacat ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt
    24061 aaaacgttcc gctaccggac cggttggttt cgtcgtcaga ccgtagaagc ggtaaaaccc
    24121 ttgagcttta cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt
    24181 aaatccacgc tggcaaaaat gctggcggga atgatagaac ctaccagcgg cgagttattg
    24241 attgacgatc atccgctgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg
    24301 atttttcagg atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aatcctggat
    24361 tttccactgc gtctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa
    24421 acgatgcgta tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca
    24481 cccgggcaaa aacagcgtct ggggctggcg cgcgcgctga tattgcgccc aaaagtcatt
    24541 attgccgatg aagccctcgc ctcactggat atgtcgatgc gttcgcagtt gattaatctg
    24601 atgcttgagt tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga
    24661 atgatgaagc acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga
    24721 ggcagcaccg ccgatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgatcgcc
    24781 ggtcattttg gcgaggcatt gacggcggat gcgtggcgta aggaccgtta gtgttctgca
    24841 cctcatcctg acagggcccc cctcaggatg aggtgttcat tacttttgat tgtcgattct
    24901 ggtgagagca accagcaatg ccagcgtata acacaccgta attgccagac tcatttttaa
    24961 cagtgtttct ccgccaaacc ccgacaatgg cgcggctata ccgccgaaga caaacatgct
    25021 catccccatc aacgcagacg ccgttccaga ttcctgcgtc cctactccac tcatagcctc
    25081 tgccccgcca acagtgccga tgccgctaca aaatgcaata gtgaaaaata atgccaccag
    25141 tgccggaatg ggcaattgtg tccatgcgca taagaccgtg agcaatgcac acaaaattgc
    25201 cgctatcagg ccacctcgca acaatgtcat cgcgttaatc cgtcgcgcca ggcgcgagaa
    25261 gatccatgaa gcaatgataa gtccgatgcc gttaaggcca aacaccaggc taaattgcat
    25321 tggactaaag ccaaattcct tctgcaacac gaacgacgaa gagccgatat atgcaaacaa
    25381 accggccagc ataaaacttt gaatcaggca aaagcgcata aaacggcggt tctgcaccac
    25441 gcttcgtccg gttaatagca atgatgagcc ccttttattt tctggcaagg tctcattaat
    25501 aaataacagg cagcccagca acagtacggt gctaatttca gccattaccc agaataaagt
    25561 gcgccagtca aaagtgctga caatgtaccc gcccagcact ggcgacaaca ccggtgccag
    25621 gccattcacc gtcattaaca gcgcaaaaaa ctgggtcaac gttactccct gatatttgtc
    25681 acgagcaata gaacgagaga gcaccgaacc acccgccccc gcgatccctt gaataaaacg
    25741 ccagaccacc agccagtaaa tattgttcgt tgtcgcgcac aaaatggaag aaacaataaa
    25801 caatagcaac gacaagatta acggacgttt acgccctatt ttgtcgctca gagggccaaa
    25861 taacagttgt ccgacgccaa gaccgatcag cgatgcggtc agggttaatt gcgttatggt
    25921 cgtggttgcc gccagttgct cagacatctc cggcaaagcc ggcaaataaa gatcggtaca
    25981 catcgggccg ataccggcta aaagaccaag aatcaatgcc catgaaagag agactctagc
    26041 catattattc cttacttgag tgatgacatg ccgccgccca gcgactgcca gagcgtgatt
    26101 cggtttgtaa aatcagtcag ttgtagtgcc agcagcgcct gctgtgcgct ccacaagctg
    26161 cgctgcgccg tcaacacggt caggtaatca ccaacacccg cctgatagcg acgcaagcca
    26221 acatcgaccg tttgttgctc agctttaaca tactggcgct gtgcatccag ttgctcttca
    26281 agcgtggtcc gccgcgccag tgcgttagcg acatctttaa atgcgctctg aacggttttt
    26341 tcataggtgg caatgagtcc acgcttttgt gcttctgcgt aacgaagctg cgccagatta
    26401 cttccgccag tgaacagcgg taatgtgacc gatggcgtaa atgaccagat ctgcattccg
    26461 tggctgaaca acgacgacaa tgcttcactg ccaacaccag cgctggcagt cagcgaaatc
    26521 gtcggaaaaa agttagcccg cgccgcgcca atatcggcat tggcgctctt caaattatgt
    26581 tccgcttcct ggatatccgg gcgacgcagc agaacgtcag atgacactcc cgcaggaacc
    26641 agacttatca tctgttccgg caggctttcc agtgttcccg gtagcaaatt ttcctcaagc
    26701 gttgtacctg ccagtaagtt cagcgcattt ttatcctgca tcacgtgtgt ctgatagctg
    26761 gcaacgctgg cgcgcgcttg ctgataaaca ctcatcgcct cactgacatc tgtcgctgcc
    26821 gccgtgccaa cctgttgctg gcgctggata attttcaatg agttttctgc actggccatc
    26881 gtttcttttg ccagggccag gttgctgtta tcagccgcca gtgtcagcca ggcggtgctg
    26941 atttcagcaa tcagcgttaa acgagtgttc tgggctgtaa attcactggc aagccaggtt
    27001 tcccgcgccg cccgagataa actctgatta cgaccaaaca gatctaacgt aaaactggac
    27061 accgtgccgt cagcctcggc agtggtttcc gtaccattcg ccagcgaacg actgcgggtt
    27121 gaacttaagg ccgcattcac cgtcgggaat aacgacgcat tagtttgccc atagagcgcc
    27181 cgtgctgagt caatatcagc aatcgctttt tgcacatcgc ggttactgtt cagtgcaata
    27241 gtcaccacct gctgcaatcg gggatcgtgg atcacctgct gccagtcatg gctgattgcc
    27301 tttccctggc cctgtgctcc cggcaaggtc gccgggattg gggattcagg cgtgctgtag
    27361 tggggctcga gcgagacaca tcccgcactc aacagcacca gaaaaataag agaacgacgc
    27421 aacataactt actcctgacg gcgcggttta ccggcaaaca aacgtttcac cagtacaaaa
    27481 aacagaggaa caaagaaaat agccagcaac gtagcggtca gcgtaccgcc aataatgccc
    27541 gtaccaatgg cgatgcggct gttcgcccct gcgccggttg ctatcgccag cggcattacc
    27601 cccgcaataa acgccagcga ggtcatgatg attgggcgta aacgagtctg cgccgcgcgt
    27661 aatgccgcac gactcaagga atagccctct gcaaccgccg cttcagcaaa ttccacaatc
    27721 agaatggcgt ttttcgacga caagccgata gtggttaata gcgccacctg gaagtaaaca
    27781 tcgttgttta aatcacgcat ccaggccgcc agcgccgcgc caagaagccc cagagggatc
    27841 accagaatga ccgagaacgg gactgaccag ctttcgtaca gtgctgcaag gcagaggaaa
    27901 accaccagaa tagaaactgc atacagactc aatgcctgac cgctggccag tttctcctgc
    27961 aatgacaaac cactccaggc ccaggttgtt cccgcaggaa gactgtttgc cagttcctcc
    28021 atttttgtca ttgcatcgcc ggaactaaag ccagtcgcat tttccccctg aatttcatag
    28081 gctgcagaac cgttgtagcg caccagtctt tccggtccat acagccagcg ggtggttgca
    28141 aaagcagaga atggtgtcat ggcgttatcg ctaccgcgca caaaccattt acctaagtct
    28201 gacggcgtgg aacgaaattc actgtcgccc tgaatgtaga cttttttcac acgaccacgg
    28261 tcaataaagt cattcacata agtaccgccc cacgcactgg acagggtgtc ggtgacatca
    28321 ttcaaactca accctaatga caccgcttta ttactatcaa tatctacctg caattgcggc
    28381 atttgtggca aatcattggc acgcacagaa tgtaactccg gactttgatt cgctttttct
    28441 atcaactgat tacgcatttg cagtagtgtt tcgcgatcgg ttccaccgtt agctaacaat
    28501 tcaaacgtaa aaccattgct ttgccccagc ccatcaacgg ctggcggcgt catcgcaaat
    28561 accgtggcat cacgaattgt gcccagctct ttggttgccc gtagggcgat agcctgggcg
    28621 gtgttttctg cccctttacg ttgagaccag tttttcaacg aaacaaacgc catcccggtg
    28681 ttctgtccgc tgccgctaaa actgaaacca tcaacggtaa aaatgacatc ggtatttgct
    28741 ttctcattaa tcaggaacca gtcaacaatc tggcgattca cttctgctgt acgggcagcg
    28801 gtcgccccgg ccggcagcgt gtactgcacc atgatttcgc cctggtcttc agtgggtaag
    28861 aaactgcccg gcagtttcca catcatcagg gccattccac cacccagtac gacataaagc
    28921 cccatcgttc gagcagcacg gcgcaggaca taaattacgc ctcgctgata tttatcttca
    28981 gtacggcggt aaaagcggtt aaatgcgccg aaaaagcctt ttttatgtgg cggaacatgc
    29041 tggaggacgg aaccgcacag ggcgggagtc aaggtcaatg ccactaccac ggaaagcagc
    29101 attgcggaga taatggttat cgaaaactga cgataaatta cccccgtaga tcccccaaag
    29161 aaggccatag gcaggaatac ggctgacaac accagcgcaa tggcaaccag tgcgccagaa
    29221 atctcgccca ttgatttttc cgtggcttca cgcgcgggca acccttcgtc acgcataata
    29281 cgttcgacgt tctccaccac cacaatggca tcgtccacca gcaagccgat cgccagcacc
    29341 attgcaaaca acgtcagggt attaatcgaa tagccaaaca acgcaagaac gccgaatgtg
    29401 cccagcaaaa cgacggggac agctaacgcc ggaatcagtg tggcacgcag gttttgcagg
    29461 aataaataca tcacgcaaac caccaatacg atggcttcaa acagcgtctg aattacgtcc
    29521 tctacagaga ttttaataaa ttcagtgctg tctttcgggt atgcgatgtc gtatccctgc
    29581 ggcatgttcc gctggaattc ggcaatctta tccttgacca gcgtcgccgt attcagcgca
    29641 ttcgcaccgg gcgaaagcat tacagcaacc ccggcagacg ggtgaccgtt aagtttgccg
    29701 atagcggtat aatcttcact gcccatctca acgcgagcca catcttttat acgaacaact
    29761 gcgccgtctg actggctttt cacgataata tttttgaact gatccaccgt ctgcaagcga
    29821 gactgcgcac gtaccgttgc ggtcagttgc tgagtattcg gtgaaggcaa tgcccctatt
    29881 ttcccggcag taacctgcac gttttgcgct tcaatagcac tttgcacgtc tgaaggcatt
    29941 agcgagtaag acgccagttt ggccgggtca agccagatgc gcatagcgta ttccgcgcca
    30001 aagacttgca gactccccac accttcaaca cgcgccagcg gatcctgaac gttactaacc
    30061 aaccagtcgg cgatatccga actggaggct ttgtcagtgg tgtcatacac ggcagcaatc
    30121 agcaaaaagt tgctttgtga tttctccacc gtaacacccg tttgctgcac ttcgctgggt
    30181 agacgcgact ccgcctgctg aattttattc tgcacctgaa cctgtgcagt atctggatcg
    30241 gtaccttgtt caaaggtcac attaatactg accgaaccat cagagctgct ggttgagctg
    30301 aaatagagta aattatcaag cccagtgagt tgttgctcga taacctgagt cacgctgttc
    30361 tccagcgttt cggcagaagc accagtataa gtggctgaaa ttttaatggt cggtggcgca
    30421 acgtcaggat attgcgcgac gggcaatgtg cgaatagcca gaatccccgc caacataatc
    30481 aaaatggcga taacccaggc aaagaccggg cggcgaacaa aaaagcgcga aaacatcagg
    30541 cgttgcctcc attagcctga acttcaacag ccttgacggt ctgccctgaa gtgactttgg
    30601 cagaaccttc aacaatcagt cggtcgccgt tgtgcaggcc gttcagcacc agccatttat
    30661 caccatacgt ttctcccgtt tcgagcgttc gctgctctac tttattgtct ttattcacca
    30721 ccagcgcagt tgcattgcct ttagcatcgc gcgtgacgcc ctgttgcggc gcgagaatag
    30781 cgtcttccat cacgccttca tcgacacgag cgcgaacaaa cattcccggt aataactgct
    30841 gttgtggatt ggggaaaatc gcccgtaatg tcaccgaacc ggtagactca tctacagcga
    30901 cttcggtgag ttccagacgc cctttttcgc tgtaggttgt gccatcttcc agaataagtg
    30961 agacgctcat ggtgtcactg ttggtcgcca gtgactgttt acgcagacgt aataaatcga
    31021 cactggagcg agtgaggtcg acatacattg tatccagacc acgaacagtc gttaacgctg
    31081 tatcttgcga cgcggtcacc agtgcgccag gggttaccga cgaaatgcca atgcgccctg
    31141 aaatcggtgc ggttaccgtg gtccagtcaa gattaatgcg cgcagtttcc agtgcggctt
    31201 ttttcgcctc tacactggct ttatcttgtg cacaggtaga ctgagcatca tcagcatcct
    31261 gttgtgaaac accgttctct ttcaccagtc gggcataacg ctgcgctttc tggcaatcgg
    31321 cttttaccag tgcctgtgct tgttgtaatg ctgcccgagc ttcattccat gcagcctgat
    31381 aactggccgc atcaatctgg tagagcggct gtccagcctt gaccagatca ccttccttaa
    31441 ataagcgttt ctggataatt cccccaacct gcggacgtac ttcagcactg agcgcagcac
    31501 tggtgcgtcc ggttaattca ctgactaccg aaaccggttg agatttgact gtaacaacgc
    31561 cgacttctgt ttctgacggg gatgaattgt tactttgcgt attatcacaa cccgataaga
    31621 gcagcattgc cactacagat gttgctatat acttcattat tttctccagg gtgagaaata
    31681 ccctcctcat taaaattaat gaggatatgt cagggatatt cagttataac gaacccgcat
    31741 cgcgaaatga atttcgcaat gaaataaggg ggattaatat cccccgctat tggttaattc
    31801 tgttcttatt tatttatcaa catactaaca atatcctgat atatttcctg cagatgttgt
    31861 ggatcgcttt tttgcggtgt taaacgacga taaaccgtac cttccatcat ggtagcaaac
    31921 acttcaacgc agcaacgaat gtgctcgtcg ctaagatggg gatgcattct tttcatatgt
    31981 gcacaaccgt tagcgaacat tctggcgtca gcttcttcaa gcattgccat aacccgcggg
    32041 ttgcgtgtgg cttctgcagc cacttccagc atcagcgcgt cgtcgtcctc atttaaggat
    32101 ttacgcaagg caaggacttc cgggaagtga tcggtacgcg cgtcaatgtc catttgggca
    32161 atacgaaagt cgatgatacg gcgaaccatc tcttcaataa tggcatcttt gttggcgaag
    32221 taacgatata tctgacccac gctaagctga gcttcgctgg caatttgcga catactggcg
    32281 gcatgaaacc cgcagcgacg aaaacaccga cgggcggcgt taatgatttc atcctgacgc
    32341 tgtttgtggc gtatctccag tttgctggtc atttctgtct cctggcctat cacttggtga
    32401 gaatgatcgt tctcattttt gctgattata cgtttctcca aagtaatgga aagtattttt
    32461 atcttattca tggtgcatat tctgataatg aagagattgt ggagattctg tgaagatagt
    32521 tgacaatatt aagttggggt caattcatca gaaaggctgc tacttgttgt cgccagttct
    32581 gttttcatca tcaaacttat cggagctaat acgaatagtt tcagagtcag attctataag
    32641 atattccccc aacctacccc ttgcaggcgt caagagtaga taacgcaaaa tgctctgtac
    32701 ctcagcttct ctccggtaca gaaagcgcaa ctatagccac ccacagccag gttgattata
    32761 ataaccgttt atctgttcgt actgtttact aaaacgacga atcgcctgat tttcaggcac
    32821 aacaagcatc aacaataagg attaaagcta tgggttttct ttccggtaag cgcattctgg
    32881 taaccggtgt tgccagcaaa ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg
    32941 aaggagctga actggcattc acctaccaga acgacaaact gaaaggccgc gtagaagaat
    33001 ttgccgctca attgggttct gacatcgttc tgcagtgcga tgttgcagaa gatgccagca
    33061 tcgacaccat gttcgctgaa ctggcgaaag tttggccgaa atttgacggt ttcgtacact
    33121 ctattggttt tgcacctggc gatcaactgg atggtgacta tgttaatgcc gttacccgtg
    33181 aaggcttcaa aattgcccac gacatcagct cctacagctt cgttgcaatg gcaaaagctt
    33241 gccgctccat gctgaatccg ggttctgccc tgctgaccct ttcctacctt ggcgctgagc
    33301 gcgctatccc gaactacaac gttatgggtc tggcaaaagc gtctctggaa gcaaacgtgc
    33361 gctatatggc gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc tctgcgggtc
    33421 cgatccgtac tctggcggct tccggtatca aagacttccg caaaatgctg gctcattgcg
    33481 aagccgttac cccgattcgc cgtaccgtta ccattgaaga tgtgggtaac tctgcggcat
    33541 tcctgtgctc cgatctctct gccggtatct ccggtgaagt ggtccacgtt gacggcggtt
    33601 tcagcatcgc tgcaatgaac gaactcgaac tgaaataatc gttctgttgg tcaagacggg
    33661 cggcgttctg ccgcccgtta tctctgttat acctttctga tatttgttat cgccgatccg
    33721 tctttctccc cttcccgcct tgcgtcagga taacgatttc ctttacgacc aaggagcgcc
    33781 catggaacaa cgccacatca ccggcaaaag ccactggtat catgaaacgc aatccagtac
    33841 tgcggaatat gacgttctgc ctctggtccc ggaagccgca aaggtcagcg atccctttct
    33901 gctcgacgtg atccttgatg aagaaacgct ggcccccttc ctttcatggc tggtccctgc
    33961 gcgcgttctt gcagtggagt tgttccctga cccgcttacc gtgacccgtt cacagacctt
    34021 caccgcttat gaacgcttgt cgacggccct gacggttgct caggtttgcg gcgtccaacg
    34081 gttatgtaac tactattcgg cgcgacttac gccgctcccc gggcctgatt cctccaggga
    34141 aagtaatcat cggttggcac aaatcacgca atatgcccgc caactggcta gctcgccttc
    34201 tattatcgac aaccgatcgc gccagcatct gaatgacgtc ggtcttactg tctgggactg
    34261 tgtgatcatt aatcaaatca ttggttttat tggctttcag gcgcggacca ttgcgacatt
    34321 tcaggcttat ctcgggcatc cggtacgctg gttacccggt ctggagatac aaaactatgc
    34381 cgacgcgtca ctgtttgctg atgaatcaat acgctggcga agcagctatg aggtggaaaa
    34441 actacctgaa gagtacacaa aaagttcaac tacagaactt tgccaactgg ccgaaacact
    34501 ctctctccac cctatttcac tttcccttct cgaaaagttg ttaaacagca cacgggttaa
    34561 tacacagccg gataatcagc ttgcggcgtt gatatgcgcg cggataaatg gcagtcctgc
    34621 ttgttttgcc gcctgtatgg attcatcaaa tgaatataaa aaaatcagcc cccttctgcg
    34681 caagggcgaa aatgaaatta accaatgggc tgaccgtcat tctgttgagc gcgctaccgt
    34741 tcaggcgata caatggctga cccgagcacc cgatcgcttt agcgccgccc agttcagccc
    34801 tttactcgaa cacgaaaaat catcaacgca gattattaat ctgctggtat ggagcgggct
    34861 gtgtggctgg ataaatcgct taaaaatcgc gttgggtgag acatattaac cttgccgcgt
    34921 cagacagatt cgcgtaaaac tgtcagccgc tctaatggcc accaaaatag acaattatgt
    34981 ttcaggacaa cccgctgcta gcgcagctta aacagcaact gcattcccag acgccacgcg
    35041 ctgaaggggt ggtaaaagcc acagaaaaag gctttggctt cctggaagtc gacgcgcaaa
    35101 aaagttattt cattccgccg ccgcagatga aaaaagtcat gcatggcgac cgaattatcg
    35161 cggttatcca cagtgaaaaa gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt
    35221 tcctgactcg tttcgtgggt aaagttcagg gcaaaaatga ccgtctggcc atcgttcctg
    35281 atcatccact cttaaaagac gccattcctt gccgcgcagc ccgtggcctg aaccacgagt
    35341 ttaaagaagg cgactgggca gttgccgaaa tgcgccgcca tccgctgaaa ggcgatcgtt
    35401 ctttctatgc tgaactgaca caatacatca cttttggtga cgaccacttt gtaccgtggt
    35461 gggttaccct tgcgcgccat aatctggaaa aagaagcacc agacggcgtc gctaccgaaa
    35521 tgctcgatga aggtctggtt cgtgaagatc tgaccgcgct ggattttgtc accatcgaca
    35581 gtgccagcac agaagatatg gatgacgccc ttttcgctaa ggcgttgccg gatggcaaac
    35641 ttcagctgat tgtggcgatt gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg
    35701 acaaagccgc gaaaattcgc gcattcacca actatctgcc tggcttcaac atccctatgc
    35761 tgcctcgcga gctttctgac gatctctgct cactgcgcgc caatgaagtc cgcccggtac
    35821 tggcatgccg catgacgctc tccgctgatg gcaccattga agataatatc gaattctttg
    35881 ctgccaccat cgaatccaaa gcgaagctgg tgtatgacct ggtttctgac tggctggaaa
    35941 ataccggtga ctggaagcct gaaagtgaag caattgccga acaagtccgt ttgttagcgc
    36001 aaatttgcca acgccgcggc gagtggcgtc ataaccacgc actggtattt aaagatcgcc
    36061 cggattaccg ctttattctc ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc
    36121 gtcgcattgc caatcgtatc gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc
    36181 gcgtactgcg cgataagctc ggttttggca tctataacgt gcatatgggc tttgatccgg
    36241 cgaatgccga cgcgctggca gcgttgctga aaacccacgg tctgcatatc gatgccgaag
    36301 aagtgctcac gctggacggt ttctgcaaac tgcgtcgtga actggatgcg caaccaactg
    36361 gtttcctcga cagccgcatt cgtcgcttcc agtcatttgc tgaaattagc actgaacccg
    36421 gtcctcactt tggcctcggt ctggaggcat acgccacctg gacttcgccg atccgtaaat
    36481 atggcgacat gatcaaccac cgtctgctga aagcggttat caaaggcgaa actgcgacgc
    36541 gtccgcagga tgaaatcact gtccaaatgg ccgagcgtcg ccgtctcaac cgaatggcag
    36601 aacgtgatgt tggcgactgg ttatacgcac gcttcctgaa agacaaagcc gggaccgaca
    36661 cccgtttcgc ggcggaaatt gtcgatatca gccgtggcgg catgcgtgtg cgtttggttg
    36721 ataacggcgc tattgccttt attccggcac ctttcttaca cgctgtgcgc gatgaactgg
    36781 tttgcagcca ggaaaacggc actgtacaaa ttaaaggtga aacggcttac aaagtgactg
    36841 acgttattga cgtcaccatt gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc
    36901 cggtcgc
//
LOCUS       DAOTRK010000030        36735 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.030, whole genome shotgun sequence.
ACCESSION   DAOTRK010000030 DAOTRK010000000
VERSION     DAOTRK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.030"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(8..2077)
                     /gene="glyS"
                     /locus_tag="QIE41_001496"
     CDS             complement(8..2077)
                     /gene="glyS"
                     /locus_tag="QIE41_001496"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDS3530398.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDDADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPAVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2087..2998)
                     /gene="glyQ"
                     /locus_tag="QIE41_001497"
     CDS             complement(2087..2998)
                     /gene="glyQ"
                     /locus_tag="QIE41_001497"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDS3530399.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3093..3401)
                     /locus_tag="QIE41_001498"
     CDS             complement(3093..3401)
                     /locus_tag="QIE41_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDS3530400.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQVSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            3576..4571
                     /gene="wecH"
                     /locus_tag="QIE41_001499"
     CDS             3576..4571
                     /gene="wecH"
                     /locus_tag="QIE41_001499"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDS3530401.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALSTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWGSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4613..5050)
                     /locus_tag="QIE41_001500"
     CDS             complement(4613..5050)
                     /locus_tag="QIE41_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDS3530402.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQITQEEYSE"
     gene            complement(5096..5437)
                     /locus_tag="QIE41_001501"
     CDS             complement(5096..5437)
                     /locus_tag="QIE41_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530403.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(5605..7059)
                     /gene="xylB"
                     /locus_tag="QIE41_001502"
     CDS             complement(5605..7059)
                     /gene="xylB"
                     /locus_tag="QIE41_001502"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDS3530404.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRLLRPAILWNDGRCA
                     QECALLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLNNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLTDISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7131..8453)
                     /gene="xylA"
                     /locus_tag="QIE41_001503"
     CDS             complement(7131..8453)
                     /gene="xylA"
                     /locus_tag="QIE41_001503"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDS3530405.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFHHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8819..9811
                     /gene="xylF"
                     /locus_tag="QIE41_001504"
     CDS             8819..9811
                     /gene="xylF"
                     /locus_tag="QIE41_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS3530406.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKETVVKDGFHKESEL"
     gene            9889..11430
                     /gene="xylG"
                     /locus_tag="QIE41_001505"
     CDS             9889..11430
                     /gene="xylG"
                     /locus_tag="QIE41_001505"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDS3530407.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11408..12589
                     /gene="xylH"
                     /locus_tag="QIE41_001506"
     CDS             11408..12589
                     /gene="xylH"
                     /locus_tag="QIE41_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDS3530408.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12667..13845
                     /gene="xylR"
                     /locus_tag="QIE41_001507"
     CDS             12667..13845
                     /gene="xylR"
                     /locus_tag="QIE41_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDS3530409.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(13953..14777)
                     /locus_tag="QIE41_001508"
     CDS             complement(13953..14777)
                     /locus_tag="QIE41_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDS3530410.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15097..17127
                     /gene="malS"
                     /locus_tag="QIE41_001509"
     CDS             15097..17127
                     /gene="malS"
                     /locus_tag="QIE41_001509"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDS3530411.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTINFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17305..18558
                     /gene="avtA"
                     /locus_tag="QIE41_001510"
     CDS             17305..18558
                     /gene="avtA"
                     /locus_tag="QIE41_001510"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDS3530412.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLRDKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPDKIEAGVKILAEEIERAWAESH"
     gene            complement(18709..19182)
                     /locus_tag="QIE41_001511"
     CDS             complement(18709..19182)
                     /locus_tag="QIE41_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDS3530413.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19284..20132)
                     /gene="yiaJ"
                     /locus_tag="QIE41_001512"
     CDS             complement(19284..20132)
                     /gene="yiaJ"
                     /locus_tag="QIE41_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDS3530414.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWKSHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20333..21331
                     /gene="yiaK"
                     /locus_tag="QIE41_001513"
     CDS             20333..21331
                     /gene="yiaK"
                     /locus_tag="QIE41_001513"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDS3530415.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21343..21810
                     /locus_tag="QIE41_001514"
     CDS             21343..21810
                     /locus_tag="QIE41_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDS3530416.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21928..22401
                     /gene="yiaM"
                     /locus_tag="QIE41_001515"
     CDS             21928..22401
                     /gene="yiaM"
                     /locus_tag="QIE41_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDS3530417.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTTPPQGA"
     gene            22404..23681
                     /gene="yiaN"
                     /locus_tag="QIE41_001516"
     CDS             22404..23681
                     /gene="yiaN"
                     /locus_tag="QIE41_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDS3530418.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLEMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23694..24680
                     /gene="yiaO"
                     /locus_tag="QIE41_001517"
     CDS             23694..24680
                     /gene="yiaO"
                     /locus_tag="QIE41_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDS3530419.1"
                     /translation="MKLRSVTYALFISGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            24684..26180
                     /gene="lyxK"
                     /locus_tag="QIE41_001518"
     CDS             24684..26180
                     /gene="lyxK"
                     /locus_tag="QIE41_001518"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDS3530420.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHKPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITSGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            26177..26839
                     /gene="ulaD"
                     /locus_tag="QIE41_001519"
     CDS             26177..26839
                     /gene="ulaD"
                     /locus_tag="QIE41_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDS3530421.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIHVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            26832..27692
                     /locus_tag="QIE41_001520"
     CDS             26832..27692
                     /locus_tag="QIE41_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDS3530422.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSATQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QNPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27686..28381
                     /gene="araD"
                     /locus_tag="QIE41_001521"
     CDS             27686..28381
                     /gene="araD"
                     /locus_tag="QIE41_001521"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDS3530423.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28413..29228)
                     /locus_tag="QIE41_001522"
     CDS             complement(28413..29228)
                     /locus_tag="QIE41_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3530424.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     AIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            29454..30854
                     /locus_tag="QIE41_001523"
     CDS             29454..30854
                     /locus_tag="QIE41_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3530425.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            30865..32829
                     /locus_tag="QIE41_001524"
     CDS             30865..32829
                     /locus_tag="QIE41_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDS3530426.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LVASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCIQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFDGKGLFRHKILIQAPGYRCEQSNPEQQPLWHYDYAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEETSA"
     gene            complement(32901..33311)
                     /locus_tag="QIE41_001525"
     CDS             complement(32901..33311)
                     /locus_tag="QIE41_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDS3530427.1"
                     /translation="MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALV
                     AHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWLLVDINMNQFEGRAERINITMPK
                     RLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV"
     gene            complement(33328..33513)
                     /locus_tag="QIE41_001526"
     CDS             complement(33328..33513)
                     /locus_tag="QIE41_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDS3530428.1"
                     /translation="MKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKD
                     IKPGTLAQIGRQAGLTF"
     gene            complement(33782..35320)
                     /gene="aldB"
                     /locus_tag="QIE41_001527"
     CDS             complement(33782..35320)
                     /gene="aldB"
                     /locus_tag="QIE41_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDS3530429.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKTRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMDEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(35521..36672)
                     /gene="yiaY"
                     /locus_tag="QIE41_001528"
     CDS             complement(35521..36672)
                     /gene="yiaY"
                     /locus_tag="QIE41_001528"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDS3530430.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         8923 a         9561 c         9266 g         8985 t
ORIGIN      
        1 gacgttatta ctgcaacagc gaaatatccg caactcgcag gaacagttcg cgcagtttct
       61 ccagcatggt cagacggttg agacgcaatt ctttgtcatc aaccatgacc atcactttat
      121 cgaagaaagc atcaactggc tcacgcagct ctgccagttc gaccagcgca tcctggtaac
      181 gaccttcagc aaagtacggc tccagtttat cgcgcagcac aaccacctgc atcgccagtt
      241 taatttcttc cggctctttc agagtagaag cattcacgcg atcgctcagc acttcgtcag
      301 acttcgccag aatgttagat acacgcttgt ttgccgccgc cagtgacgct gccgcatcca
      361 gagtacggaa gtgcgatact gccttcatac gggcatcaaa atctgccgga cgcgtcggac
      421 gacgtgccag taccgcctgg attgtgtcaa cggtgtaacc ttcgtcctga taccaggcgc
      481 ggaagcgacc gagcataaag tcgataacat catcaaccac gttggcatta gtcagcttat
      541 cgccatacag acgcactgct tcttcggtca gcgtttgcag atcaaggttg aggttcttct
      601 caacgataat acgcagtacg ccaagtgcgg cacgacgcaa cgcaaacggg tctttgtcgc
      661 ctttcggatg ctggccgata ccgaagatac ccgccagagt atccatcttg tcagcaatcg
      721 ccagcgcaca ggctaccggg ttagacggca ggtcatcacc ggcaaagcgc ggctgatact
      781 gctcgttcag cgccactgca acatcttccg cttcgccatc gtgacgcgcg tagtgcatcc
      841 ccataacgcc ctgggtgtcg gtgaactcga agaccatgtt ggtcatcagg tcgcacttag
      901 acagcaggcc cgcacgggta gcatggttaa cgtcagcgcc aatctgttca gcaatccagc
      961 cagccagtgc ctggatgcgg tcagttttgt cacgcagtgt acccagctgt tgctggaaca
     1021 gcacggtttg caggcgcggc aggttatctt ccagacgttt tttacggtcg gtattgaaga
     1081 agaactcagc atccgccaga cgtggacgaa cgactttctc gttaccggag ataatctgct
     1141 gcggatcttt cgattcgatg ttggcaacga agataaagtt cggcagaagt ttgccgtcgt
     1201 tcgcatacac cgggaagtac ttctggtcac ctttcatggt gtaaaccagc gcttcagacg
     1261 gcaccgcgag gaatttctct tcgaattttg cggtcagcac agccggccat tccaccagcg
     1321 aagcgacttc ttccaacagg ctttcgctta agtcagcgtt accgccaatc ttacgcgccg
     1381 cttcttcagc atcggcttta atcttcgctt tacgttcttc gtaatcggcg atgactttac
     1441 cgcgctcacg cagaatttcc ggatactgat cggcatcgtc gatagtgaat tccggctcgc
     1501 ccataaagcg gtggccgcga atcacgcgat cggactgaat gcccagaatg gtagccggaa
     1561 tgactttgtc gcccagcagc agggtcacgg tgtgcaccgg acgcacgaag tgcacgtcgc
     1621 ttgcgcccca gcgcatcagt ttcgggatcg gcagtttcgc cagcgaagtc gcaaccatat
     1681 tcggcagcag tgcttcggtg ctttcgccct tcacgtgggc gcgatacagc agccattcac
     1741 ctttatcggt agtcagacgc tcagcctggt ctacggtaat accgcaacca cgcgcccagc
     1801 cttctgccgc tttgctcggt ttgccttcag cgtcgaacgc ctgagcaatc gccgggccgc
     1861 gtttttcgat ttcacgatcc ggttgcgctt ccgccaggtt agctactttc agcgccagac
     1921 gacgcggagc agcaaaccat tgaacggtgc cgtgtgcgag gccggcgtta tccagctccg
     1981 cagtgaagtt cgcagcaaag gactcagcca ggctgcgcag tgcttttggt ggcagctctt
     2041 cagtgccgat ttccaccaga aaagttttct cagacatagc cgcctcttac ttatctttgt
     2101 tgcacatcgg gaagccgagg gcttcacggg aagcgtagta tgcttcagcc actgctttgg
     2161 tcagggtgcg aattcgcaga atgtagcgct gacgctcggt gacggagatg gctttacgcg
     2221 catccagcag gttgaagctg tgggcggctt tcagaatacg ctcgtaggct ggcagcggca
     2281 gcggattttc cagcgccagc agctgctgcg cttctttctc gtactgctca aagcaggtga
     2341 acaggaagtc cacatccgcg tattcgaagt tgtaagtgga ctgctccact tcgttctgat
     2401 ggaacacgtc gccgtaggtg gttttaccca gcgggccgtc gctccagacc aggtcgtaaa
     2461 cgctgtctac gccctgaatg tacattgcca gacgttccag accgtaggtg atctcgccgg
     2521 taaccggttt acactccaga ccaccaacct gctggaagta agtgaactgc gtcacttcca
     2581 tgccgttcag ccacacttcc cagcccagcc cccaggcacc cagcgtcggg ttttcccagt
     2641 tatcttccac gaaacggatg tcgtggatgg tcgggtccat gcccagctct ttcagagaac
     2701 caaggtacag ctcctgaata ttgtccggcg atggcttaat gaccacctgg aactgatagt
     2761 agtgctgtaa acggttgggg ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct
     2821 gaacataagc agccgccatc ggttctggcc ccagcgcgcg cagacaggtc attgggtgag
     2881 aggttcccgc gccgacttcc atgtccaatg gttgaacaat ggtgcagccc tggcgagccc
     2941 agtaatcctg taaggtcagg atcaagccct ggaaggtcct ggtatcaaac ttttgcatat
     3001 tatttcgtgc tggatacgtg tggatttaaa ggaagggatc agtatacccg ctggatggaa
     3061 gatatacagt acgaaacggg aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg
     3121 ccatctgcgt caaaagaaca gacaaactgc tctttacgga aggtataacc gcgcatttca
     3181 tagcttacct gaaattgttc aaaagcagtg acgtcgattt tttgctcgcc ggtgttataa
     3241 cgttttgccg cctgatcctt gcataacgct tccatattca acgaacgcag aggatcaacc
     3301 tttacccgtt gtgctttctg cacgggtggc ggtgtagaac aacccactag cgccataagc
     3361 accataagcg ccattgccgg aaagaaagag ttcatcatca ttaaattacc gtctggtttg
     3421 caggtgagtc ttattattta tatcggtata gtggctgata tcttacgaat ttccttaagc
     3481 ctctataagc tgtagtgata aaactcagat tattccagtg cgccaaccga atgatttatc
     3541 ggtcagactt tttttaacca gatacagaga cacagatgca gcccaaaatt tactggattg
     3601 ataacctgcg agggatagcg tgtttaatgg tggtgatgat tcacaccact acctggtatg
     3661 tgaccaatgc tcatagtgtt agccccgtca cctgggatat cgccaatgtt ctgaattctg
     3721 cctctcgtgt cagcgtgccg ctatttttca tgatttccgg ctatctcttt tttggcgaac
     3781 gcagcgccca gccgcgccat tttttacgta tcggcttatg tctgcttttt tatagcgcaa
     3841 tcgcactgct ctacattgcg ctgtctacct ccatcaatgt ggagttagcg ctgaaaaacc
     3901 tgctgcaaaa gccagtgttt taccacttgt ggtttttctt cgcgattgcg gtgatttatc
     3961 tggtttcacc gctgattcag gtgaagaacg tcggcggaaa aatgttgctg gtgctaatgg
     4021 tggtgattgg tattatcgct aacccaaaca cagtgccgca gaaaattgac ggttttgaat
     4081 ggctgccaat taacttatat atcaatggcg atacttttta ctacatcctt tatggcatgt
     4141 tgggccgcgc tatagggatg atggacacac agcataaagc actgtcgtgg gggagcgccg
     4201 cactgtttgc gacgggggtt tttattatct ctcgcgggac attatatgaa ttgcagtggc
     4261 gcggaaattt tgccgatacc tggtatcttt actgtgggcc gatggttttt atctgcgcaa
     4321 tcacgctatt gactctggtt aaaaacacgc ttgatacgcg taccgttcgc ggacttggct
     4381 taatctcccg ccattcgttg ggtatatacg gattccacgc cttgattatc catgcactgc
     4441 gcacccgggg gattgagctt aaaaactggc caatactcga tattatttgg atcttttgcg
     4501 cgacgttggc agcgagtttg ttactttcta tgctggtaca acgaatcgac agaaacagac
     4561 tagtgagtta aataaaagcc cggtcacatt ggactgaccg ggcttatgtg aattattcgc
     4621 tgtattcttc ctgggtaatc tgtgtttctt ttggtgggtt tattccggca tcacgaatat
     4681 tcttctgcac cgctactgca ccaaacaagc ttaagaagaa agccagtcca taaaaacctt
     4741 tctcgctgag taataacgtc gcattccaca gacctaccat cagtaacgca acagagataa
     4801 taaagacagt caggcaggtc atataataaa tggaggtggt cggtatgcct tcatacttgt
     4861 cccgaacggt cttttgataa gacgccgcag aaaacagtcc taataccagt acggcaaaat
     4921 aatatccttt ttcatttaac tgcatctctg cattccatag ccctaacaga taggtaacga
     4981 taccaccaac gagagctatc catgacacaa tactgaatgc cggagaatag gttgatattt
     5041 tgttgtccat cacagtattc ctttcattcc tgaatgtgta agaggtttat gttgctcatg
     5101 ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg ggataaatca
     5161 ccatttcata agcattgata ggtgcgttta aaacaccgat aaacaagaga ccgatagtga
     5221 tcaacgcaac taactggcag atatggataa aaaaatcatc cagttgcccg agtttttctg
     5281 cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga aaatagcctt
     5341 ttccacttaa caatgggcat gagatccata gcccaaccag ataaaccagc gccccaacaa
     5401 caaataatag ttttgcaaca gtctttgagg ttttcataac gatctccata tctaccagcg
     5461 atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca aatgatttca
     5521 gaataaattc atactaaata ttaattaatt actgatatat atagatgtga attatccccc
     5581 acccggtcag gcaggggata acgtttacgc cattaatggc agaagttgct gatagaggcg
     5641 acggaacgtt tctcgtcgtg gctgataagc ggcataacgc tgcgcatctg gtagatgcga
     5701 ctgttctaac ggcagttgtg gcaacaactc aatgagcgat ttctctggat tcgccgcaat
     5761 ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc ccccccgtgc ggtaatcgag
     5821 ttgctgaccg ctgatatccg tcagcatctg acgccagtac tcactacgcg ccccgccacc
     5881 aatcaacgta acactttgcg gtttaatgcc gcaggcatgc acgacatcca tgccatctgc
     5941 cagcgcataa cccacgcctt ccagcactgc tcgcgccagt tcattggggc catgttgatg
     6001 agtcaaacca aagaaaaccc ccttcgcctg gggattattg tgtggtgtac gctcgccgga
     6061 aagataaggc agaaaccaaa ctggctcggc actttcatca gcctgttgag cagccgcgat
     6121 taaagctggg acattgttca ggcccgttaa tttcgcagcc caatccagac acgacgctgc
     6181 actcagcatc acagacatta aatgccaacg ctgcggtagc gcatggcaaa agctgtgtac
     6241 ggcgctttct ggcttgctta agaacccttc gctgacagca aaatagactc ccgacgtccc
     6301 cagcgataac attgcctgat tagcatcaac cattcctaca ccaaccgcac cagctgcatt
     6361 atcaccaccg cctgcgacaa ctggcaccgc cgccataccc cacgctttcg caacttcagg
     6421 tagcaaagca ccagtaattt cgctgccttc gtataatgcg ggcatctggt cacgagataa
     6481 gtggcaagcc tgcagcatgt cgtcactcca gtcacgcttt gcgacatcca gccacatggt
     6541 gccagctgcg tcagacatat cgctggcaaa ctcccccgtc atacgcagac gcaagtaatc
     6601 tttcggtaac aatactttgt cgatttgacg gaatatctcc ggctcatgcc gctgaaccca
     6661 tagcaattta ggcgcagtaa atccgggcat catcaggtta ccggtaatca cccgcgattg
     6721 cggaactcgc gcttccagca aagcgcactc ttgcgcacag cgcccgtcgt tccacaaaat
     6781 ggcagggcgt aataaccgtt gctgggcatc cagtaaggtt gcaccgtgca tctggccggc
     6841 aatacccaat gctttaacgt cctgcagaga atgctgatcg cccagggctt tcattgcgcg
     6901 atcagttgcc tgccaccact gttccgggtc ttgttccgac cagagtggat gcgggcgcga
     6961 aacggtcagc ttttccgttt gcgcagcaac cacctcgccc tgctcgttga gcaaaataac
     7021 ttttacgccc gaggtgccaa gatctatccc gatatacata tcgatcgttc cttaaaaaaa
     7081 tgcccggtat cgctaccgat aaccgggcca acggactgca cagttagccg ttatttgtcg
     7141 aacagataat ggtttaccag attttccagt tgctcctggc gaccactctg atgcaccgga
     7201 gacaaattgt gttcctgagc atatttggct aaatctgcca gtgacatttg gcctttcagg
     7261 atttgctggc ctaattcgct attccagccg gaataacgct gcgcgatgcg tttatccagc
     7321 tcgccatctt caatcatgcg tgctgcaatt ttcagcgcca acgccatcgt atccatcgcg
     7381 ccgatatgac cgtaaaacag atcatattta tcagtacttt gacgacgtac tttggcatcg
     7441 aagttcagac cgccggtggt gaaaccgcct gctttgagaa tttcatacat caccaacgca
     7501 ttctcttcca cactgttcgg gaactggtcg gtgtcccagc ccagttgtgc atcgccacgg
     7561 ttagcgtcga cagaaccgaa caggccaagc gcaatggcgg tggctatttc atgatggaaa
     7621 gagtgacctg ccagcgtcgc gtggttagct tcaatgttca gtttaatctc tttttccaga
     7681 ccaaactgtt tcaggaagcc atagactgtc gcagcatcgt aatcatattg atgtttggtc
     7741 ggttcttgcg gtttcggttc gataagcaac gtaccctgaa agccgatttt atgtttatgc
     7801 tcaaccacca tttgcataaa gcggcccagc tgttcacgct cctgacgcaa gtcggtattt
     7861 aacaacgttt cgtaaccttc acgaccgccc cacaggacat agttttcacc gcccaattta
     7921 tgggttgctt ccatcgctgt aacaacttgc gttgccgccc agctgaagac ttctggatct
     7981 gggttcgtcg ccgcacccgc gccgtagcga gggtttgtaa agcagttagc ggttccccac
     8041 agcagcttca cgccgctctc ttcttgcttg cctgccagga catcaaccat ttgcgcaaaa
     8101 ttattgatgt actcttttaa cgacgcgccc tcaggggaaa catccacgtc gtggaagcaa
     8161 taaaatggca catgtaactt gtggaaaaac tcaaatgcga catctgcttt acgcttcgcc
     8221 aacgccagtg cctcaccagg ctgctgccac ggacgattaa acgcccccac accaaacatg
     8281 tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg caaaacgcaa gtgctcttcc
     8341 atacgcttgc ccaacaccag ttcgtcggga ttatagtgat ggaatgctaa cgggttagag
     8401 gattttgagc cttcataacg aacgcgatcg agctggtcaa aataggcttg catattgaac
     8461 tccataatca ggtaatgccg cgggtgatgg atgatgtcgt aatattgggc actccctttc
     8521 agttgctcaa ttatgttatt tcacactgct attgagataa ttcacaagtg tgcgctcgct
     8581 cgcaaaataa aatggaatga ggaaactggg taattcctcg aagagaaaaa tgcaataagt
     8641 acaattgcgc aacaaaagta agatctcggt cataaatcaa gaaataaacc aaaaatcgta
     8701 atcgaaagat aaaaatctgt aattgttttc ccctgtttag ttgctaaaaa ttggttacgt
     8761 ttatcgcggt gattgttact tattaaagct gtcctctacc tacagaaggc cctacaccat
     8821 gaaaataaag aacattctac tcaccctttg cacctcactt ctgcttacca acgttgcggc
     8881 acacgccaaa gaagtcaaaa taggtatggc gattgatgat ctccgtcttg aacgctggca
     8941 aaaagatcga gatatttttg tgaaaaaggc agaatctctc ggcgcgaaag tatttgtaca
     9001 gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt gaaaacatga ttaaccgggg
     9061 cgtcgatgtt cttgtcatta ttccgtataa cggtcaggta ttaagtaacg ttgtaaaaga
     9121 agccaaacaa gaaggtatta aagtattagc ttacgaccgt atgattaacg atgcggatat
     9181 cgatttttat atttctttcg ataacgaaaa agtcggtgaa ctgcaggcaa aagccctggt
     9241 cgatattgtt ccgcaaggta attacttcct gatgggcggc tcgccggtag ataacaacgc
     9301 caagctgttc cgcgccgggc aaatgaaagt gttaaaacct tacgttgatt ccggaaaaat
     9361 taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg gaaaacgcat tgaaaattat
     9421 ggaaaacgcg ctaaccgcca ataataacaa aattgatgct gtagttgcct caaacgatgc
     9481 caccgcaggt ggggcaattc aggcattaag tgcgcaaggt ttatcaggga aagtagcaat
     9541 ttccggccag gatgcggatc tcgcaggtat taaacgtatt gctgccggta cgcaaactat
     9601 gacggtgtat aaacctatta cattgttggc aaatactgcc gcagaaattg ccgttgagtt
     9661 gggcaatggt caggaaccaa aagcagatac cactctgaat aatggtctga aagatgttcc
     9721 ctcccgcctg ctgacaccga tcgatgtgaa taaaaacaac atcaaagaaa cggtagttaa
     9781 agacggattc cacaaagaga gcgagctgta agcgttacgc cccggcgggg agcgggggcg
     9841 tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg gagtcgttat gccttatcta
     9901 cttgaaatga agaacattac caaaaccttc ggcagtgtga aggcgattga taacgtcagc
     9961 ttgcggttga atgctggcga aatcgtctca ctctgtgggg aaaatgggtc tggtaaatca
    10021 acgctgatga aagtgctgtg tggtatttat ccccatggct cctacgaagg cgaaattatt
    10081 tttgcgggag aagagattca ggcgagtcac atccgcgata ccgaacgcaa aggtatcgcc
    10141 atcattcacc aggaattggc cctggtgaaa gaattgactg tgctggaaaa tatcttcctg
    10201 ggtaacgaaa taacccacaa tggcattatg gattatgacc tgatgacact acgctgtcag
    10261 aagctgctcg cacaggtcag tttatccatt tcacccgata cccgcgttgg cgatttaggg
    10321 cttgggcaac aacaactggt tgaaattgcc aaggcactta ataaacaggt gcgtttgtta
    10381 attctcgatg aaccgacagc ctcattaact gagcaggaaa cgtcggtttt actggatatt
    10441 attcgcgatc tgcaacagca cggtatcgcc tgtatttata tttcgcacaa actcaacgaa
    10501 gtcaaagcga tttccgatac gatttgcgtt attcgcgacg gtcagcacat tggtacgcgt
    10561 gatgctgccg gaatgagtga agacgatatt atcaccatga tggtcgggcg agagttaacc
    10621 gcgctttacc ctaatgaacc acataccacc ggagatgaaa tattacgtat tgaacatctg
    10681 acggcatggc atccggttaa tcgtcatatt aaacgagtta atgatgtctc gttttccctg
    10741 aaacgtggcg aaatactggg tattgccgga ctcgttggtg ccggacgtac cgagaccatt
    10801 cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa aaatttatat tgatggcaaa
    10861 caggtagata ttcgtaactg tcagcaagcc atcgcccagg ggattgcgat ggtccccgaa
    10921 gacagaaagc gcgacggcat cgttccggta atggcggttg gtaaaaatat taccctcgcc
    10981 gcactcaata aatttaccgg cggtattagc cagcttgatg acgcggcaga gcaaaaatgt
    11041 attctggaat caatccagca actcaaagtt aaaacgtcgt cccccgacct tgctattgga
    11101 cgtttgagcg gcggcaatca gcaaaaagcg atcctcgctc gctgtctgtt actcaacccg
    11161 cgcattctca ttcttgatga acctaccagg ggtatcgata ttggcgcgaa atacgagatc
    11221 tacaaattaa ttaaccaact cgtccagcag ggtattgccg ttattgtcat ctcttccgaa
    11281 ttacctgaag tgctcggcct tagcgatcgt gtactggtga tgcatgaagg gaaactaaaa
    11341 gccaacctga taaatcataa cctgactcag gagcaggtga tggaagccgc attgaggagc
    11401 gaacatcatg tcgaaaagca atccgtctga agtgaaattg gccgtaccga catccggtgg
    11461 cttttccggg ttgaagtcac tgaatttgca ggtcttcgtg atgattgcag ctatcatcgc
    11521 aatcatgctg ttctttacct ggaccaccga tggtgcctac ttaagcgctc gtaacgtctc
    11581 caacctgtta cgccagaccg cgattaccgg catcctcgcg gtaggaatgg tgttcgtcat
    11641 aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg gggctattag gtggcgtcgc
    11701 ggcgatttgt gacgtctggc taggctggcc tttgccactt accatcattg tgacgttggt
    11761 tctgggactg cttctcggtg cctggaacgg atggtgggtc gcgtaccgca aagtcccttc
    11821 atttattgtc accctcgcgg gcatgttggc atttcgcggc atactcattg gcatcaccaa
    11881 cggcacgact gtttccccca ccagcgccgc gatgtcacaa atcgggcaaa gctatctacc
    11941 cgccagcacc ggcttcatca ttggcgcgct tggcttaatg gcttttgttg gctggcaatg
    12001 gcgcggaaga atgcgccgtc aggctttggg tttgcagtct ccggcctcta ccgcagttgt
    12061 cggtcgccag gcattaacgg cgattatcgt cttaggcgca atctggctgt tgaacgatta
    12121 ccgtggcgtt cccactcctg ttctgctgct gacgttgctg ttactcggcg gaatgtttat
    12181 ggcaacgcgg acggcatttg gacgacgcat ttatgccatc ggcggcaatc tggaagcagc
    12241 acgtctctcc gggatcaacg ttgaacgcac caaacttgcc gtattcgcta ttaacggatt
    12301 aatggtcgct atcgccggat taatcctcag ttctcgactt ggcgctggtt caccttctgc
    12361 gggaaatatc gccgaactgg acgcaattgc cgcatgcgtg attggcggca ccagtctggc
    12421 tggcggtgtg ggaagcgttg ccggagcggt aatgggggca ttcatcatgg cttcactgga
    12481 taacggtatg agtatgatgg atgtaccgac cttctggcag tatatcgtta aaggtgcgat
    12541 tctgttgctg gcagtatgga tggactccgc aaccaaacgc cgttcttgat tttgataaaa
    12601 attttctcaa agccggtaac gtattaccgg ttttgagttt ttgcatgatt cagcaggaaa
    12661 agaaccatgt ttactaaacg tcaccgcatc acattactgt tcaatgccaa taaagcctat
    12721 gaccggcagg tagtagaagg cgtaggggaa tatttacagg cgtcacaatc ggaatgggat
    12781 attttcattg aagaagattt ccgcgcccgc attgataaaa tcaaggactg gttaggagat
    12841 ggcgtcattg ccgacttcga cgacaaacaa atcgagcaag cgctggctga tgtcgacgtc
    12901 cccattgttg gggttggtgg ctcgtatcac cttgccgaaa gttacccacc cgttcattac
    12961 attgccaccg ataactatgc gctggttgaa agcgcatttt tgcatttaaa agagaaaggc
    13021 gttaaccgct ttgcttttta tggtcttccg gaatcaagcg gtaaacgttg ggccactgaa
    13081 cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt atcgcggagt ggtttatcag
    13141 gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa atcggctggc agactggcta
    13201 caaacgctgc caccgcaaac cgggattatt gccgttactg acgcccgggc gcggcatatt
    13261 ctgcaagtat gtgaacatct acatattccc gtaccggaaa aattatgcgt gattggcatc
    13321 gataacgaag aactgacccg ctatctgtcg cgtgtcgccc tttcttcggt cgctcagggc
    13381 gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc gattattaga taaagaagaa
    13441 atgccgctac agcggatttt ggtcccacca gttcgcgtca ttgaacggcg ctcaacagat
    13501 taccgctcgc tgaccgatcc cgccgttatt caggccatgc attacattcg taatcacgcc
    13561 tgtaaaggga ttaaagtgga tcaggtactc gatgcggtcg ggatctcgcg ctccaatctt
    13621 gagaagcgtt ttaaagaaga ggtgggcgaa accatccatg ccatgattca tgccgagaag
    13681 ttggagaaag cgcgcagtct gctgatttca accaccttgt caatcaatga gatatcgcaa
    13741 atgtgcggtt atccatcgct gcaatatttc tactctgttt ttaaaaaagc atatgacacg
    13801 acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt tgtagcgatg atgagaattg
    13861 tcggcgtcgc atcaggtaac tcagcacaac cacctgatgc gagcaaaagc cttatccgac
    13921 tgacaagtcg gataaggctc gaggaaatgc aattacatat gcgccgcgat taaccgttgg
    13981 ttatcctggt acattgcgaa caggtagttg ttataactct tccctttggt cgagtagccc
    14041 ttcagtttgt gaatcatcgc tgtggcagtc acttcctgat ccgctttacg cagctgtgca
    14101 cgcgatttac ggaacgaaga gtaagccggg tgagtattca ggttagtgac ataggcgctc
    14161 accgattctt tgacagaact aaactgtgag taccccttca ctttacccgg cgcattagta
    14221 caacgtcctt tcatgcattt catgccgaac aggttgttgt tgttgcgcgc cagcttcgac
    14281 gttccccaac cgctttctgc tgcagccatc gtcgccacca tactagtggg gataatgtct
    14341 acgcgttcaa gcaaggtatt ccacgggatt ttgcgcgtat taccagacca cttcaccttg
    14401 tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag gtgaccattg gccctgatac
    14461 tgttttgaaa tgagccagtt acgttccgca gtaatggccg cattttggct ggtaatgtaa
    14521 ggcattacag tccggagaaa cgcctttttc cttggtgttc cggaagggta ttttcgcaaa
    14581 tcaggaagtg aactactctt tgcactattg cgagaatact cttgtttact gcttacctgt
    14641 ttattactag ctttagttaa gtgggacttt tgactcgctg ttgttgtgtg cgtcttcgct
    14701 aacacctcac tcgaaaacac cagagtgagt aacataagaa tcatcgcccc atatcgtcgt
    14761 atgggagtca aaatcatcag gtctcctggt cggatttaat cattccaaca ccttatattt
    14821 ttcacaaatt tgagagttga atctcaaatc atatcaaaaa tagctgtcaa gagcacccca
    14881 aggaatagtc caaatctgaa actatgtcac gagttaacga ttcagattgg cgctaaatcg
    14941 cagaaaatgt ggggtttatc gcaaaattca gccgtttttt gcgcgcgatc gctcaccctt
    15001 gcttctcatc ctgtggactt accgctcagg gatgagtttt gtctggctta tcgctggcaa
    15061 actgtctgaa atcgcagcaa taaggactca tccgccatga aactcgccgc ctgttttctg
    15121 acgctccttc ctggcttcgc cgttgccgcc agctggactt ctccggggtt tcccgccttt
    15181 agcgaacagg ggacgggaac atttgtcagc cacgcgcagt tgcccaaagg tacgcgtcca
    15241 ctaacgataa attttgacca acagtgctgg cagcctgcgg atgcgataaa actcaatcag
    15301 atgctttccc tgcaaccttg tagcaacacg ccacctcaat ggcgattgtt cagggacggc
    15361 gaatatacgc tgcaactaga cacccgctcc ggtacaccaa cattgatgat ttccatccag
    15421 aacgccgtcg aaccggtagc aagcctggtc cgtgaatgcc cgaaatggga tggattaccg
    15481 ctcacactgg atgtcagcgc cacttttccg gagggagccg cagtccggga ttattacagc
    15541 cagcaaattg cgatagtgaa gaacggtcaa ataacgttac aacccgctgc caccagcaac
    15601 ggtttactcc tgctggaacg ggcagaaact gacgcccctg cccctttcga ctggcataac
    15661 gccacggttt actttgtgct gacagatcgt ttcgaaaacg gcgatcccag taatgaccag
    15721 agttacggac gtcataaaga cggtatggca gaaattggca cttttcacgg cggcgattta
    15781 cgcggcctga ccaacaaact ggattacctc cagcagctgg gcgtcaatgc tttatggata
    15841 agtgccccat ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg cgatttcccg
    15901 cattatgcct accacggtta ttacacacag gactggacga atcttgatgc caatatgggc
    15961 agtgaagccg atctacggac actggttgat agcgcgcatc agcgcggtat tcgtattctc
    16021 tttgatgtcg tgatgaacca caccggctat gccacactgg cggatatgca ggagtatcag
    16081 tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa cgttgggtga acgctggagc
    16141 gactggaaac ctgccgccgg gcaaacctgg catagcttta acgattacat taatttcagc
    16201 gacaaaacag gctgggataa atggtgggga aaaaactgga tcagaaccga tatcggcgat
    16261 tacgacaatc cgggattcga cgatctcact atgtcgctgg cctttttgcc ggatatcaaa
    16321 accgaatcaa cgaccgcttc tggtctgccg gtgttctata aaaacaaaac ggatacccac
    16381 gctaaagtca tcgacggctt tacccctcgc gattacttaa cccactggtt aagtcagtgg
    16441 gtccgcgact atgggattga tggttttcgg gtcgacaccg ccaaacatgt tgagttgccc
    16501 gcctggcagc aactgaaaac cgaagccagc gccgcgcttc gcgaatggaa aaaagctaac
    16561 cccgacaaag cattagatga caaacctttc tggatgaccg gtgaagcctg gggccacggc
    16621 gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga tgatcaattt cgattatcag
    16681 gagcaggcgg ctaaagcagt cgattgtctg gcgcagatgg atacgacctg gcagcaaatg
    16741 gcggagaaat tgcaggattt caacgtgttg agctacctct cgtcgcatga tacccgcctg
    16801 ttccgtgaag ggggcgacaa agcagcagag ttattattat tagcgccagg cgcggtacaa
    16861 atcttttatg gtgatgaatc ctcgcgtccg ttcggcccca ccggttcgga tccgctgcaa
    16921 ggtacgcgtt cggatatgaa ctggcaggat gttagcggta aatctgccgc cagcgtcgcg
    16981 cactggcaga aaatcagcca gttccgcgcc cgccatcccg caattggcgc gggcaaacaa
    17041 acgacacttt cgctgaagca gggctacggc tttgttcgtg agcatggcga cgataaagtg
    17101 ctggtcatct gggctgggca acagtgactt ttccggcttc ccgttcgtca gtacctcggg
    17161 aagccgccaa ccaggataaa atgtcagagc taatcagcgt tgcaggataa agcaccgctc
    17221 actcttcaac agagcgattt gcaccccagc aaatgtagcg ttattgttac cttccttgct
    17281 acagagttcg acagatatcc cgctatgaca ttctcccttt ttggtgacaa atttacccgc
    17341 cactccggca ttacgctgct gatggaagat ctgaacgacg gtttacgcac gcctggcgcg
    17401 attatgctcg gcggcggtaa tccagcgcag atcccggaaa tgcaggacta cttccagacg
    17461 ctactgaccg acatgctgga aagtggcaaa gcgactgatg cactgtgcaa ttacgacggt
    17521 ccacagggga aaacggagct actcacactg cttgccggaa tgctgcgcga taagttgggt
    17581 tgggatatcg aaccacagaa tattgcacta acaaacggca gccagagcgc gtttttctac
    17641 ttatttaatc tgttcgccgg acgccgtgcc gatggtcggg tcaaaaaagt gctgtttccg
    17701 ctggcaccgg agtacattgg ctacgctgac gccggactgg aagaagatct gtttgtctct
    17761 gcgcgtccga atattgaact gctgccggaa ggccagttta aataccacgt cgattttgag
    17821 catctgcata ttggcgaaga aaccgggatg atttgcgtct cccggccgac gaatccaaca
    17881 ggcaatgtga ttactgacga agagttgctg aagcttgacg cgctggcgaa tcaacatggc
    17941 attccgctgg tgattgataa cgcttatggc gtcccgttcc cgggtatcat cttcagcgaa
    18001 gcgcgcccgc tgtggaatcc gaatatcgta ctgtgcatga gtctttccaa gctgggtcta
    18061 cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa tcatcaccgc catcaccaat
    18121 atgaacggca ttatcagcct ggcacctggc ggtattggtc cggcgatgat gtgtgaaatg
    18181 attaagcgta acgatctgct gcgcctgtct gaaacagtca tcaaaccgtt ttactaccag
    18241 cgtgttcagg aaactatcgc catcattcgc cgctatttac cggaagatcg ctgcctgatt
    18301 cataaaccgg aaggagccat tttcctctgg ctatggttta aggatttgcc cattacgacc
    18361 gagcagctct atcagcgcct gaaagcacgc ggcgtgctga tggtgccggg gcacaacttc
    18421 ttcccagggc tggataaacc gtggccacat acacatcaat gtatgcgcat gaactacgtg
    18481 ccggagccgg ataaaattga ggcgggtgtg aagattctgg cagaagagat agaaagagcc
    18541 tgggctgaaa gtcactaaaa attgcctgat gcgctacgct tatcaggcct acatgatttc
    18601 tgcaatatat tgaatttggt agaatttgta ggccggataa ggcgttttag ccgcatccgg
    18661 cataaacaaa gcgcactatt ccggcgttga gaaacgccgg aaaacgtttc aaacaggctg
    18721 ctgccgtagc cgatgccgct gcactttctc gacatccatg cactgcaacg cctttgtcgg
    18781 gcaggcttca acacaggccg gccccgtctc ccgatgccag cacagatcgc acttaatcgc
    18841 cctcgccttt ttgcgcgaag aaacgacctc catcgcacca aacgggcaag ccagcataca
    18901 gcttttacag ccaatgcaac gtgattgttc aacgaaaata tgcccatgtt cgcggcttat
    18961 cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca cactgatgac aggctaccgc
    19021 cgtggtccag ctgtggtctt taatgacacg aatacgggaa ataaactcgt ctggtgacaa
    19081 cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa gccacttcac aggtacggca
    19141 accgatacat ttcgtcgcat ccgcaataat aaaccggttc atgatcactc ctttgcacta
    19201 aaacagcaga gtgccagaga gcgcgtctct actgctttga tccaacgaag gaaatgttaa
    19261 agttttggca cttgtccaaa gcgttatgtg atcgcgccct ggtcgtccct gacagtaaat
    19321 cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg gtttcaggag atttttctcc
    19381 cccacctgtt tcagacgtga tgtcgaaaga gaaatcgaca cggcgtacgg cacccgccca
    19441 tgaatatcaa acaccggaac agcaatacag gagacgccga gttcgttttc ttctctgtcc
    19501 atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga acatcgcggg cagctcggta
    19561 atggtattgc gggttaacgg ctggatctca tgctgatggc ttttccagta tgacttcacg
    19621 tagtccgggt gaccaaacgc catgtagatc ttgcccattg cggaacagta gagcggcata
    19681 tgctggccta tataggcacg ggttcgcagc atcccggttg tgggttccag cttataaatc
    19741 aaaatagcgt gatcgtcttc gcggctggag aagttaatgg tttcaccagt ggcgatgttc
    19801 agtgcctcaa gatgcggagc ggcgatatga atgatattca gcgaagacag cgccttctgc
    19861 ccgacggcaa taaatttggt agtcaggcgg taactccctg cggcgggcgc ggtggtcaca
    19921 tagccgcagg actgtaatcc ctgcaataag cgatggacgg tactcttatt taaaccagcc
    19981 agctccgaaa gatgcgccaa cggacaaccg tttggatagt tgctcaaaat ctcaatcagc
    20041 atcaacccgc gaaacagact ctggcttccg gctggacgct ctttttcctg cgccatctcg
    20101 ttctcttttt ttcccatcac ttctttcccc attttgtcgc gtcctgatgg tagcgcaaag
    20161 tgtgtcgtag ttcacgatct cgacagataa tttataacca cttgattttt aagttttttg
    20221 aaattcatca atcagattgc tttgttaaaa agcgatcgat atatttgaaa tcagatttcg
    20281 catattgaaa ttttaagcca aaaaagcgat caaaaaaaca aggaagccag ggatgaaagt
    20341 gacatttgag cagttaaaag cagcctttaa tcgagtctta atttcacgcg gcgttgacag
    20401 cgaaacggct gatgcctgtg ccgagatgtt cgcccgcacc accgaatccg gcgtttattc
    20461 tcacggcgtc aaccgtttcc ctcgtttcat tcaacaactg gaaaacggcg atatcattcc
    20521 tgatgcccaa cccaaacgta taaccagcct cggcgcaatt gaacagtggg acgcccagcg
    20581 ttcgatcggt aacctgacag cgaaaaagat gatggatcgc gccatcgaac tggctgccga
    20641 tcatggtatt ggtctggtgg cactacgtaa tgccaaccac tggatgcgcg gcggcagcta
    20701 cggctggcag gcggcggaaa aaggctatat tggcatttgc tggaccaact ccatcgccgt
    20761 aatgccgccg tggggcgcaa aagagtgtcg catcggcacc aacccgctga tcgtcgccat
    20821 tccttctacc ccaatcacca tggtcgatat gtcgatgtcg atgttctctt acggcatgtt
    20881 agaagttaac cgtctggcag gtcgtcagct cccggttgat ggtggctttg atgatgaggg
    20941 caatttgacc aaagaacctg gcgttatcga gaagaatcgc cgcattttgc cgatgggcta
    21001 ctggaaaggt tctggcatgt cgattgtgct ggatatgatc gccactctcc tttccgacgg
    21061 cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa tacggcgttt cgcaaatctt
    21121 tatcgccatc gaagtggata aattgatcga cggccccacc cgcgatgcaa agctgcaacg
    21181 catcatggat tacgttacta ccgctgaacg cgctgacgaa aaccaggcca tccgcttacc
    21241 cggccacgaa tttactaccc tgctggccga aaaccgccgt aacggcatca ccgttgatga
    21301 cagcgtgtgg gccaaaatcc aggcgttatg aggagataag tcatgatttt tggacatatc
    21361 gcgcaaccca atccgtgccg tttgcctgcc gccattgaaa aggcgctcga ttttctgcgc
    21421 gctaccgatt tcaacgccct ggagccgggt gttgtcgaaa tcgacggcaa gaatatttat
    21481 gcgcaaatta tcgatttaac cacacgcgaa gcggcggaaa accgtccgga agtgcatcgt
    21541 cggtatatcg atattcagtt tctggcgtgg ggtgaagaga aaatcggcat tgctattgat
    21601 acgggaaata ataaagtcag cgaatcacta ttagagcagc gcgatattat tttttatcac
    21661 gatagtgaac atgaatcatt tatagaaatg ataccgggca gctacgctat tttcttcccg
    21721 caggatgttc atcgaccagg ttgtattctg caaacagcct ctgagatacg aaaaattgtg
    21781 gttaaagtcg cattaacagc gctgaattaa tcgaataatc gtctacgcca gaacgcctgg
    21841 gtaatgttat tgctctaact atattaataa tttaggttgc tgcacatgca acttgaatta
    21901 tgacagacat agcctcaagg aatagctatg aaaaaaatac ttgaagcaat actggcgatt
    21961 aatctcgccg tactttcctg tattgtgttt ataaacatta ttttaagata tggttttcag
    22021 acaagcattt tatctgtaga tgaattgtca cgttatttat ttgtctggtt aacatttatt
    22081 ggcgcgattg tggcttttat ggataacgcc cacgttcagg tcacctttct ggtggagaaa
    22141 ctctcccccg catggcagcg acgagtcgct ctggttaccc attccttaat cttatttatt
    22201 tgtggcgcac tggcctgggg cgcaacgcta aaaacaattc aggactggag cgattattca
    22261 ccgatcctcg gtttgcccat cggcctgatg tatgccgctt gtctgcccac cagcctcgtt
    22321 atcgcattct ttgaactgcg tcatttatat caactcatca cgcgtagcaa ttctctaacg
    22381 acgccaccgc aaggagctta atcatggctg tgctgatttt tctgggctgt ctgttgggtg
    22441 gtattgctat cggtttgcct attgcctggg cactgttgtt gtgcggggcg gcgttaatgt
    22501 tctggctgga gatgtttgat gtccagatca tggcacaaac gctggtgaac ggagccgaca
    22561 gcttctccct gctggcgatt ccgttctttg tgctggcggg tgaaatcatg aatgcgggcg
    22621 gcttgtcaaa acgcattgtt gacctgccga tgaaactggt ggggcataaa ccaggcgggc
    22681 tgggctacgt cggcgtgctg gcggctatga ttatggcaag cctttctggt tctgccgttg
    22741 cggataccgc cgccgttgcc gctctactgg tgccgatgat gcgcagcgcc aactacccgg
    22801 tcaaccgggc ggcggggctg attgcctctg gcggcattat cgcgccaatt attccccctt
    22861 ccattccgtt tattattttc ggcgtttcca gcggattatc catcagtaag ctgtttatgg
    22921 cgggcattgc ccctggcatg atgatgggcg caacgctgat gcttacctgg tggtggcagg
    22981 ccagtcgcct taatttaccc cgccagcaaa aagcaacgat gcaggaaatc tggcactctt
    23041 ttgtctccgg tatctgggcg ctgtttcttc cagtcatcat tattggcggg tttcgctccg
    23101 gtctgtttac ccctaccgaa gccggggccg ttgctgcttt ctatgcgttg tttgtcgcca
    23161 cagttattta ccgtgaaatg accttcgcca cactttggca tgtgcttatt ggcgcggcga
    23221 aaacgacctc agtggtgatg tttctggtgg cctcggcaca agtttccgcc tggctgatca
    23281 ccattgctga actgccgatg atggtttctg atttactgca accgctggtc gattcaccgc
    23341 gtctgctgtt tatcgtcatt atggtggcta tcctgattgt cggcatggtc atggatttaa
    23401 cgccgacggt attaattctt accccggtat taatgccttt agttaaagag gcaggaatcg
    23461 atccgattta tttcggtgtc atgtttatca ttaactgctc aatcggctta attacaccgc
    23521 ctatcggtaa tgtgcttaac gttatttcag gggtggcaaa actcaaattc gatgatgcag
    23581 tcagaggcgt tttcccttac gtcctggttt tatattcatt attagtcgta tttgttttta
    23641 ttcccgatct catcatcctc cctttgaaat ggattaatta aaaggaaaat attatgaaat
    23701 tacgctctgt aacctacgca ttattcattt ctggcctggc tgcattcagt acatcttcac
    23761 tggcggcaca atctttacgt ttcggttatg aaacctcaca aaccgactcg caacatattg
    23821 cagcgaaaaa attcaatgaa ttattacagg aaaaaaccaa aggcgaactg aaattaaaat
    23881 tgtttccgga tagcaccctc ggtaatgcac aggcgatgat cagcggcgtg cgtggcggca
    23941 ccatcgatat ggaaatgtcc ggctcgaata actttgccgg attgtcacct gtgatgaacc
    24001 tgcttgatgt ccctttcctg ttccgcgata ccgcccacgc gcataaaacg ctcgacggca
    24061 aagtcggtga tgacctgaaa gtctcccttg aaggtaaggg actgaaagta ctggcctatt
    24121 gggaaaacgg ctggcgcgat gtcaccaact cgcgtgcgcc agtgaaaaca cctgccgacc
    24181 tgaaagggct gaaaattcgc accaacaata gtccgatgaa tatcgccgca ttcaaagtct
    24241 ttggcgctaa cccgatcccg atgccgtttg ccgaagtcta taccgggctg gaaacccgca
    24301 ctatcgacgc tcaggaacac cctatcaacg tcgtctggtc agcaaaattt ttcgaagtgc
    24361 agaaatacct ttctctgacc catcacgcct attccccgct tctggtggtg atcaacaaag
    24421 cgaagtttga cggcttaacc ccggagttcc agcaggcgct gatctcatct gcgcaggaag
    24481 cgggtaacta ccagcgcaaa ctggttgccg aagatcagca aaaaatcatc gacggcatga
    24541 aagaagcggg cgtggaagtc atcaccgatc tcgaccgcaa agcctttagc gacgcactgg
    24601 gtactcaggt ccgcgacatg tttgttaaag atgttccgca gggcgctgag ctgctgaaag
    24661 ccgtggatga ggtgcaataa accatgacgc aatactggct ggggttagat tgtggcggta
    24721 gctggctgaa agccgggctg tatgaccgcg aaggccggga ggcaggcgtg cagcgcctgc
    24781 ctctgtgcgc attaagcccg cagccaggct gggcagagcg cgatatggca gaactgtggc
    24841 aatgctgcat ggctgtcatt cgcgccctgc ttactcattc tggtgtcagc ggggagcaaa
    24901 tagtgggtat cggtatctcc gcacagggaa agggcttgtt tttgctggat aaaaacgaca
    24961 agccgctcgg gaatgctatt ttgtcctcgg accgccgggc gatggaaatc gttcgtcgct
    25021 ggcaggaaga tggcatcccg gaaaaacttt acccgctgac ccgacaaacc ttgtggaccg
    25081 ggcatccggt gtcgctgtta cgctggctga aagagcacaa accagaacgc tacgcgcaaa
    25141 ttggctgcgt gatgatgacg cacgactacc tgcgctggtg tttaaccggc gtcaaaggct
    25201 gtgaagagag caatatttcc gagtccaacc tctacaacat gagtcttggg gaatatgacc
    25261 cgtgcctcac cgactggctt gggatcgctg aaatcaacca cgccctgccg cctgttgtcg
    25321 gatctgccga aatctgcggg gagatcaccg ctcagacagc cgcactgacc ggtctgaaag
    25381 cgggtacgcc cgttgtcggc ggcctgtttg atgtggtttc taccgcactc tgcgccggga
    25441 tcgaagacga atttaccctc aatgcggtaa tgggcacttg ggcggtgacc agcggcataa
    25501 ccagcggttt gcgtgacggt gaagcgcatc cgtatgtcta tggtcgctac gttaacgatg
    25561 gtcaatttat cgttcacgaa gccagcccca cctcttccgg caacctcgaa tggtttaccg
    25621 cacagtgggg agaaatctcg tttgatgaga tcaaccaggc cgttgccagc ttgccgaaag
    25681 ccggtggcga tctctttttc ctgccgttcc tgtacggcag caacgccgga ctcgagatga
    25741 ccagcggttt ctacgggatg caggccattc acacccgcgc acacctgttg caggccattt
    25801 atgaaggggt ggtattcagc catatgaccc atctcaaccg gatgcgcgaa cgttttactg
    25861 atgtgcacac cctgcgcgtc actggcggcc cggcgcattc cgatgtctgg atgcaaatgc
    25921 tggcggacgt cagcggactg cgtatcgagc tgccgcaggt ggaagaaacc ggctgttttg
    25981 gcgcggcgct tgccgcccgc gtcggcaccg gggtttatca caacttcagc gaagcccaac
    26041 gtgatttaca gcacccggta cgcaccctgc tgccggatat gaccgcccat cagctttacc
    26101 aacaaaaata ccaacgctat cagcatctca ttgccgcact tcagggcttt cacgcccgca
    26161 tcaaggagca cacattatga gccgaccact tctgcaactg gccctcgacc actcatcact
    26221 tgaagccgcg cagcgcgacg tgacgctgtt aaaagacagc gtcgatatcg tcgaagcggg
    26281 caccattctc tgtttaaacg aagggcttgg cgcagtgaaa gccttacgcg aacaatgccc
    26341 ggacaaaatc atcgttgctg actggaaggt cgccgacgct ggtgaaacgc tcgcgcaaca
    26401 ggcgtttggc gcgggtgcca actggatgac catcatctgc gccgcgccgc tcgcgacggt
    26461 ggaaaaaggc cacgcgatgg cgcaacgctg cggtggtgaa attcagatcg agttgttcgg
    26521 taactggacg ctggacgacg cccgcgactg gcatcgtatt ggcgtgcggc aggccattta
    26581 tcatcgcggt cgtgacgcac aggccagcgg gcaacagtgg ggcgaggccg atctggcacg
    26641 catgaaggcg ctttccgata tcggccttga gctttccatt accggcggga ttacccctgc
    26701 tgacctgccg ctgtttaaag atatccacgt gaaagcgttt attgccggac gagcactggc
    26761 aggcgcagca aatccggcgc aggtggctgg cgatttccat gcgcaaattg atgccatctg
    26821 gggaggcgcg cgtgcgtaat catccgttag ggatttatga aaaagcgctg gcgaaagatc
    26881 tctcctggcc tgagcggctg gtgctggcaa aaagctgcgg ctttgatttt gtcgaaatgt
    26941 cggtggatga aaccgacgaa cgtttgtcac gccttgactg gagcgccaca caaaggactt
    27001 cgctggttgc cgcgatgatc gaaacgggtg ttgggatccc gtcgatgtgc ctgtccgcac
    27061 atcgtcgctt tccttttggt agccgtgacg acgcggtacg ccagcgagca cgggagatca
    27121 tgagtaaagc gattcgtctg gcgcgcgatc tcggcattcg caccattcaa ctggcgggtt
    27181 atgacgtcta ttacgaagac cacgacgaag gcacccagca acgttttgca gaagggctgg
    27241 cgtgggcggt cgaacaggcg gcggcatcac aagtcatgct ggcagtggag attatggata
    27301 ccgcgtttat gaactccatc agcaaatgga aaaaatggga cgagatgctc gcctcgccgt
    27361 ggttcaccgt ctacccggac gtcggcaacc tcagcgcctg gggcaatgat gttcccgccg
    27421 aactgaaact gggcattgac cgtatcgccg cgatccacct gaaagacacc cagccagtca
    27481 ccgggcaaaa tcccggacag ttccgtgatg tgccgtttgg cgaaggctgc gtcgatttcg
    27541 ttggcatctt caaaacgctg catgaactga actatcgcgg ttctttcctg attgagatgt
    27601 ggaccgaaaa agccaaagag ccggtgctgg agattattca ggcgcggcgc tggattgaag
    27661 cgcgtatgca ggaggctgga tttatatgtt agagcaactg aaagccgacg tgctggcggc
    27721 gaatctggcg cttcccgctc accatctggt gacgttcacc tggggcaatg tcagcgcggt
    27781 agacgaagcg cggcaatgga tggtaatcaa accttccggc gtcgagtacg acgtgatgac
    27841 cgccgacgat atggtggtgg ttgagatagc cagcggtaag gtggtagaag gcagcaaaaa
    27901 gccctcgtcc gacacgccaa cgcatctggc gctttaccgt cgctatgccg aaatcggtgg
    27961 cattgtgcat acccactcgc gccacgccac catctggtca caggccgggc tggatctccc
    28021 cgcctggggc accacgcacg ccgattattt ctatggtgcc atcccctgca cgcgacagat
    28081 gaccgcagag gagatcaacg gcgagtatga ataccagacc ggcgaagtga tcattgaaac
    28141 tttcgaagaa cgcggcagga gtccggcgca aatcccggcg gtactggtgc attctcacgg
    28201 cccgttcgcc tggggtaaaa acgccgccga tgcggtgcat aatgccgtag tgctggaaga
    28261 gtgcgcctat atgggcctgt tctcgcgcca gcttgcgccg caactccctg cgatgcaaaa
    28321 cgaactgttg gataagcact acctgcgtaa gcatggggcc aatgcctatt acgggcagta
    28381 atccctcacg ccggggcttc atcgccccgg cactacgaat tgatatgttc cttgctgtaa
    28441 cgtcgcttcc acgcagctgg cgttaaaccg gtgtgtttgc gaaaaatctg tcggaaataa
    28501 ccaacgtcat taaacccgca caacgccgcc acttccgtga gcgatcgcgc ttcgctaatt
    28561 aacagctttt cggcttcgcg cactcgctgg cgatggattg cttctgttaa cgtcagatga
    28621 aacacccgcc gatagacacg cccaagataa tcggcattgc agtacagctc gcgggctaaa
    28681 agcgccgtag aaagcgggcg atgatattgc gtgcggatca gtttctgcgc tttccaggca
    28741 agcgtggtac ctgcggcatc ggcctgttca tcaagacgcg cgtcacgggt gagttgctgc
    28801 aaaatcagca gcacaataaa ctccagcgcc ggagtgcgtt gatgcttttc ctgttcgctt
    28861 aagaactggc gaaacagggc caccagcgcc tgcggatcgg caaccttcgt ttgttgcggc
    28921 acgctaagat gagtgagcca gcgcgggtcg ctatccggcg ctttaacctc aaaatgcagc
    28981 cagtaaaatt tgagatcggc gggaaaatcc tctaccccaa catgctgtcg gttcggccag
    29041 agtaataaac tctctcccgc atcgaccaga aatatccgct cttcttcctg aatcgctaat
    29101 cgaccttttt cgacaaagat aatttcccat gactccagcc gccttgccgg atgcctccct
    29161 acgccacggg aaataaataa tccaccattt tgcactttaa tcggtaacgt catggataat
    29221 tcgagcatta atatgtaatt tcccgcttat tcattaatga ttaaacacat taccttttga
    29281 ttattaatca aaaacattaa tttcacctga tccaattcac acttttaaat tcaggtcgga
    29341 atcgtcacct ttttttactc ttttctcgcc ttgttgatat tcccgttcac ggcagaatta
    29401 atcatgtcat tgcaataaaa ctaaaaattg caccctattc acgaggaaca tctatgacct
    29461 ctacaccgat tactaccgct gatatcgcta aaggtaaaat tgacgatgcg ttatctgtac
    29521 gggaaaaaat aggctacggc ctgggtgacg caggcggcac cgtaataact tgcctgatca
    29581 tgaactttct cacctttttc tacaccgacg tttttggctt aactccggcg ctggtcggca
    29641 cgctgtttat tgcactgcgc gtgtttgatg ccatctccga cccggtgatg ggcgtcattg
    29701 ccgaccggac gcaaagtcgc tgggggcgct ttcgtccgtg gcagttgtgg attgccattc
    29761 ccatcggcat tatcggcatc ctgacgttca ccgtgccaga tgccagcatg ggagtaaaaa
    29821 tcgcctgggc gttcggcact tacctgctcc tttcagtcgg ttataccgcc atcaacgtgc
    29881 cgtactgcgc gctgatcaac accatgacca cccgccacaa tgaagtgatc tcctgccagt
    29941 cctggcgatt cgttctctgc ggcgtggcgg gatttctggt ttcggtaggc ttaccgtgga
    30001 tggtagatct cttcggtcag ggtaatgccg cgcgcggcta tcaactgggc gtcggcgtat
    30061 tgtgcgccat tgccgtggtg atgttcctgt gctgtttctt ctgggttcgt gaacgggtgc
    30121 cactctccac aatggggaaa tttaccctgc gcgaacatct tgccgggctg cgaaacaacg
    30181 accaactgct gctgatgctg gtcatgtctt tcctgctgat taacgtcttt aatattcgcg
    30241 gcggtgggta tatgtacttc attacctacg tcttacaagg cagtacgggc tacacgtcgc
    30301 tgttcttcac catggtcacc ttcgcctcca ttatcggctc ggtgattgtc agcccattaa
    30361 cgcggcgttt cgataccgtc aaaatttact actacaccaa cctgctcctc gctgcgctgg
    30421 cggtgttgat gtggttcctg ccttctggcc cggcttatca aacgctgtgg ctggcggtga
    30481 tcctcggtaa tggcgtgatt cttggcttca cattgccact gcacttctca ttgatggcct
    30541 ttgccgatga ctacggcgag tggaaaaccc gcgtacgttc ttccggcatg aacttcgcct
    30601 tcaatctgtt tttcatcaag ctggcctggg cctccagcgc cgggatcatc agcctgctgt
    30661 ttatttttgt cgcctaccag ccaggcgtgg aaaaccagac cgccagttcg cttggcggga
    30721 tcgcggcgat ggaaacattg ctgcctgcgc tattccacct gctgctggcg atggcgatcc
    30781 gcttttgcaa actcaataac cctatgatgt cacgcatagc tagcgacctg cgtcagcgtc
    30841 atgtacagcc ttaaggagca caagatgaac atttcggaag tcgatctgca taaactgacg
    30901 gtcagcgatc cgttcctcgg tcagtaccaa caactggtcc gcgacgtggt gattccttat
    30961 cagtgggatg ccttgaacga tcgtatccca gaagcggaac ctagccatgc gattgaaaac
    31021 tttcgcattg ccgcaggact gcaagacggt gaattttacg ggatggtgtt tcaggacagc
    31081 gacgtcgcca aatggctgga agcggtagcc tggtcgctgt gccagaagcc ggatgccgaa
    31141 ctggaaaaaa ccgctgacga ggtaatcgaa ctggtcgctt ccgcccagtg cgaagatggc
    31201 tatctcaata cttactttac ggtaaaagcg cccgaagaac gctggagcaa tctggcggag
    31261 tgtcatgaac tttactgcgc cggtcatctg attgaagccg gagtcgcctt cttccaggcc
    31321 acgggcaagc ggcgcttgct ggaggtcgtt tgccgcctgg ccgatcatat cgacagcgta
    31381 tttggtccag gtgaaagtaa gttacacggt tatcccggtc acccggaaat tgaactggca
    31441 ctaatgcgcc tgtatgaagt gactgaagag ccgcgctacc tggcgctgac gaactatttt
    31501 gtcgaacagc gtggtgcgca accgcactat tacgaccagg aatatgaaaa gcgcgggcag
    31561 acctcgcact ggcacaccta cggcccggcg tggatggtga aagacaaagc ctacagccag
    31621 gcgcatttgc ccatcgcaca gcagcaaacc gccattggtc acgcagtacg ttttgtctac
    31681 ctgatgaccg gcgtcgcgca tctcgcgcgt ttaagtcacg atgaaagcaa gcgtcaggac
    31741 tgcctgcggc tgtggaacaa tatggcccag cgtcagttat atattaccgg cggcattggc
    31801 tcacaaagca gcggcgaagc gttcagcagc gattacgatc tgccgaatga cacggtatac
    31861 gccgaaagct gtgcttccat cggcctgatg atgttcgccc ggcgaatgct ggaaatggaa
    31921 ggcgacagtc aatatgccga tgtgatggag cgcgcactgt acaacaccgt gctcggcggc
    31981 atggcgctgg atggcaaaca tttcttctat gtgaatccgc tggaagtgca tccaaaaacg
    32041 ttaaaattca accatatcta cgatcacgtt aagccgatcc gccagcgttg gtttggttgc
    32101 gcttgttgtc cgccaaatat cgcccgcgtg ctaacctcga ttggtcatta tctctacacg
    32161 ccgcgtgaag atgcgttgta tatcaacatc tacgcaggaa acagcatgga agtgccggta
    32221 gaaaatggca cgctgcgtct gcgggttagc ggaaactatc cgtggcagga acaggtgaca
    32281 attgcggttg aatcgcccca gccggtgcgt catacgctgg ctttacgtct gccggactgg
    32341 tgcatacagc cgcagatcat attgaatggg gaagaggtcg agcaggatat tcgtaaaggg
    32401 tatttgcaca ttacccgcga atggcaggag ggcgacacgc tgaatctgac tttgccaatg
    32461 ccggtacgtc gcgtttacgg taaccctctg gtgcgtcacg tcgccggaaa agtggcgatt
    32521 cagcgcggcc cgctggtgta ttgcctggaa caggccgaca acggcgagtc actgcataac
    32581 ctgtggctgc ctaccgatgc gccatttacg acctttgacg gcaagggatt gttccgccat
    32641 aagatcttaa tccaggcacc gggttaccgg tgtgaacaga gcaatccaga acaacaaccg
    32701 ttgtggcatt acgactatgc cccagccaaa cgccagccgc aaactctgac atttatcccg
    32761 tggtttagct gggctaaccg gggtgaaggc gaaatgcgga tctgggtgaa tgaggaaacc
    32821 agcgcatgat gcgacgctta acgcgtctta tcagtatcaa catatcggtg cggttgcccg
    32881 ccaaccatcg aaacgcgaga ttaaacgccc ggcaatacac gccgggcagc ttctgccaga
    32941 aacgcacttc tgttggcgta gtctgggtta ttgcgcacgt aggtatcaat cttgttcagt
    33001 aaccttttcg gcatcgtaat attgatacgt tcagcccgac cttcgaactg attcatattg
    33061 atatccacca gcaaccattg cccaccaata aagtcctgcg cacgctggac cagatgtgtt
    33121 tccacagagc ctggaagcgg caactcttca ttcatctcgc ataaggtttc gaagtgcgcg
    33181 accagtgcac ttcgcgcatc ctggaaagcg ttgtccagcg tatcaccagc aaaataacag
    33241 cctggaacat cagggaaaaa accgctggcg ctgccgtcag gatcactgtg gatataggcg
    33301 ggataaaaca tattgcctcc tgaaatttta aaatgttaaa ccagcctgac gcccaatttg
    33361 cgccagcgta ccgggcttaa tatctttttt agggtgcgga accgtcacca gacccggatg
    33421 aacagggtgg cggaactgat gatgactgcc acgggttcga atatgttccc agccatgctg
    33481 cttcaggatg gcgatcacgt ctgcactttt cacatccctg cccttccatg attatacaca
    33541 cgatacacac acaattagcg ataacaatca cgatagcgcg atttatatgg gccgtcaggg
    33601 ggataaagtt gactcgctcg gaaggagaat aacggtgtgc agtaacttgt tgaaaagatg
    33661 cgaggagact cgtaattaag ttcaatcaat attcagtcat tcaagacgta gacatttgaa
    33721 ttggtaaaac gcgcaaacgt cgcatcaggc aatgaatacc cgatgcgacc agcttcttac
    33781 atcagaacag ccccaacggt ttatccgagt agctcaccag caggcacttg gtttgctggt
    33841 aatgctccag catcatcttg tgggtttcac ggccgatacc tgattgtttg tagccaccaa
    33901 acgccgcatg tgccgggtaa gcgtgataac agttggtcca cacgcgcccg gcctgtatgc
    33961 cgcgccccat cttataggcc agattaccgt tgcggctcca gacgcccgcg cccaggccat
    34021 attgcgtgtc attcgccagc tccagcgcct cgtccattgt tttgaaggtg gttaccgcca
    34081 gcaccgggcc aaaaatctcc tcctggaaca cgcgcatatt attctgacca aacagaatcg
    34141 tcggttcgag gtagtagccg tctttcagtt cgccttccag cagcttgcgc cgcccgccgg
    34201 tgagcacgtc tgcgccctct tttttaccaa tatcaatgta gttgaggatg gtttccagtt
    34261 gcccgtgaga aacctgcgcg cccatttgcg tcacgctgtc gagcgggtta ccgctacgaa
    34321 tactttcaac acggcggatg gcgcgttcca tgaagcgttc gtagatagac tcctgcacta
    34381 aagcacggct tggacaggtg caaacttcgc cctggttaaa ggcaaacagt gcaaagcctt
    34441 ccagcgcttt atcgaaaaag gcatcttctt catccatcac atcagcaaag aagatatttg
    34501 gcgacttacc gcccagttcc agggttaccg gaataatatt ttgcgtggcg tactgcataa
    34561 tttgttggcc cacttccgtt gagccggtaa acgccacttt ggcgatgcgt ttcgaggtcg
    34621 ccagatattc gccaatttcc ccaccagcgc cgttaaccac gttcaccacg cccggcggca
    34681 gtaaatcacc gacgatttcc attagcagca gtacagaaag cggggtaaga cgtgcgggtt
    34741 tcagcaccac acagttgccc gccgccagcg cgggagccat tttccagctc gccatcagca
    34801 gcgggaagtt ccacgggata atctgcccca ccacgcctaa cggttcatgg aaatgatagg
    34861 ccacggtttc gctatcaact tcactgatcc cgccttcctg cgcccgaata cacgaggcga
    34921 aatagcggaa atggtcaatc gccagcggta catcagcagc actggtttcg cgaatgggtt
    34981 tgccgttatc ccaggtttca gccgtcgcta acagttcaag gttttgttcc attcgatcgg
    35041 caatcttaaa cagaatcgcc gcacgatcct gcaccgaagt atgcgcccat ttatctttca
    35101 ctttgtgcgc agcatccagc gccagatcga tgtctcgttt gcccgaagac gccacttcgc
    35161 acagcagctg cccggtcacc ggcgtcaggt tctggtaata ctcaccatcg gcaggggcta
    35221 cccattcgcc gccaataaag ttgtcatagc gggtttttaa cttgaggggg aaaccatact
    35281 cgccgggctt aatctgtgct gaagggggat tattggtcat gatcgtctcc ttgcggtgtg
    35341 aggtataaca agggtagacg tggctggcga aatcttcgcc agtcggtaac agctttacga
    35401 cggctatcac gaatttacgg gcaaggatta cagagataac taaaactctc tcaaaaatcg
    35461 caaattttga gatagtttaa aaaatggctg acaattagcc agccattggg ggaatgatga
    35521 ttacatcgca gcgcgataaa ttgccacaat ctcttcgtga gttgcctgaa tcgggttagt
    35581 aaacccacac gcatctttca gggcattggt ggccagtacc gcgaaatcct cttctttcac
    35641 gttcaggtca cgcaggcctg ccgggatatt cactttcttc gccagttcac ggatggcatt
    35701 gatgcacgct tctgcgcctt ccgcgtcatt tttgcctgtc acgttaacgc ccatggcagc
    35761 agcacagtca cgcagacgcg cagcagcgac tttgctgttg aacacctgaa cgtgtggcag
    35821 cagaacagca ttacatacac cgtgtggcag gttgtagaaa ccgcccagtt ggtgcgccat
    35881 cgcatgaaca taaccaagag atgcgttgtt gaacgccata ccagcgagga actgggcata
    35941 agccattgct tcacgcgctt tagcattgct gccatcttca acggctaacg gcaggttttc
    36001 ggcaatcatg gtcaccgctt tcagtgcaca agcgtcagtg atcggcgttg cagcgataga
    36061 aacatacgct tcgatagcgt gtgttaaggc atccataccc gttgcggcgg tcagtgactt
    36121 cggcataccg atcatcagcg aggagtcatt gacagaaagc aacggagtta catgtttatc
    36181 aacaatcgcc attttgatat gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc
    36241 agaggccgta cccgccgtgg tattgatggc gatcatcggc agctgcggtt ttgcagagcg
    36301 gtcaacgcct tcgtaatcgc gaatatcgcc accattggct gccaccagcg caataccttt
    36361 tgcgcagtcg tgtggagaac caccgcctaa ggaaatcacg ctatcgcaat tattttcttt
    36421 aagtaatttc aaacctgcag cgacgttttc cgtggtgggg ttaggttggg tgccatcata
    36481 aataacgcta aaaatattgc gttcttccag tgctttttgc acatcacccg ccatacccaa
    36541 tttcgttaac atattgtcag tgacaattaa ggtacgggta aatccataat ctgccatcat
    36601 attcattgca tcattcaatg aatcagcacc aataacattc acggaaggaa taaaaaaggt
    36661 tgaagctgcc ataacacact cctgaaagtg taaaaaaggg cgggcagctt actgaggatt
    36721 ttcattcaaa aatat
//
LOCUS       DAOTRK010000031        36702 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.031, whole genome shotgun sequence.
ACCESSION   DAOTRK010000031 DAOTRK010000000
VERSION     DAOTRK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.031"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(21..773)
                     /gene="yecC"
                     /locus_tag="QIE41_001529"
     CDS             complement(21..773)
                     /gene="yecC"
                     /locus_tag="QIE41_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDS3530431.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(770..1438)
                     /gene="tcyL"
                     /locus_tag="QIE41_001530"
     CDS             complement(770..1438)
                     /gene="tcyL"
                     /locus_tag="QIE41_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDS3530432.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFVLALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1453..2439)
                     /gene="dcyD"
                     /locus_tag="QIE41_001531"
     CDS             complement(1453..2439)
                     /gene="dcyD"
                     /locus_tag="QIE41_001531"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDS3530433.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGVPALFAYHPHV"
     gene            complement(2544..3344)
                     /gene="tcyJ"
                     /locus_tag="QIE41_001532"
     CDS             complement(2544..3344)
                     /gene="tcyJ"
                     /locus_tag="QIE41_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS3530434.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3432..3983)
                     /gene="fliZ"
                     /locus_tag="QIE41_001533"
     CDS             complement(3432..3983)
                     /gene="fliZ"
                     /locus_tag="QIE41_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDS3530435.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHGQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4029..4748)
                     /gene="fliA"
                     /locus_tag="QIE41_001534"
     CDS             complement(4029..4748)
                     /gene="fliA"
                     /locus_tag="QIE41_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDS3530436.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGSVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5068..6756)
                     /locus_tag="QIE41_001535"
     CDS             complement(5068..6756)
                     /locus_tag="QIE41_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDS3530437.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSQSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSSTLNLTGFNVNGSGSVANTAATKADLTAAQLSAPSAADANGTVTYTVSAGYKE
                     STAADVIASIKDGSAPTSAITATINNGFGDSSALTSNDYTYDSVKGDFTYNVASSANN
                     TAAQVQSFLTPKAGDTANLKVTVGTTSVDVVLASDGKITAKDGSALYIDNTGNLTQNS
                     AGLTSADLATLTGLQSSSVASTITTEDGTNIDIAANGNIGLTGVRISADSLQSATKST
                     GFTVGTGATGLTVGTDGKVTIGGTTAQSYTSKDGSLTTDNTTKLYLQKDGSVTNGSGK
                     AVYVEADGDFTTDAATKAATTTDPLAALDDAISQIDKFRSSLGAIQNRLDSAVTNLNN
                     TTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7022..8428
                     /gene="fliD"
                     /locus_tag="QIE41_001536"
     CDS             7022..8428
                     /gene="fliD"
                     /locus_tag="QIE41_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDS3530438.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKEPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8453..8863
                     /gene="fliS"
                     /locus_tag="QIE41_001537"
     CDS             8453..8863
                     /gene="fliS"
                     /locus_tag="QIE41_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDS3530439.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8863..9228
                     /gene="fliT"
                     /locus_tag="QIE41_001538"
     CDS             8863..9228
                     /gene="fliT"
                     /locus_tag="QIE41_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDS3530440.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVEPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9306..10793
                     /gene="amyA"
                     /locus_tag="QIE41_001539"
     CDS             9306..10793
                     /gene="amyA"
                     /locus_tag="QIE41_001539"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDS3530441.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10827..11240)
                     /locus_tag="QIE41_001540"
     CDS             complement(10827..11240)
                     /locus_tag="QIE41_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDS3530442.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11427..12632
                     /gene="yedE"
                     /locus_tag="QIE41_001541"
     CDS             11427..12632
                     /gene="yedE"
                     /locus_tag="QIE41_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDS3530443.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12629..12862
                     /gene="yedF"
                     /locus_tag="QIE41_001542"
     CDS             12629..12862
                     /gene="yedF"
                     /locus_tag="QIE41_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDS3530444.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12969..13637
                     /locus_tag="QIE41_001543"
     CDS             12969..13637
                     /locus_tag="QIE41_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDS3530445.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFVDG
                     WFEWKKEGDKKQPYFIYRADGQPVFLAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQDIGGKEASEIATSGCVPANQFTWHPVSRAVGNVKNQGAAL
                     IQPV"
     gene            13748..14227
                     /locus_tag="QIE41_001544"
     CDS             13748..14227
                     /locus_tag="QIE41_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS3530446.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
     gene            complement(14676..15479)
                     /locus_tag="QIE41_001545"
     CDS             complement(14676..15479)
                     /locus_tag="QIE41_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDS3530447.1"
                     /translation="MFPLNDLSLKAQSVQLNKITSNTESTIKQQDLVSDDAMINELSS
                     ELVSCLGNGRFTPISEDSKLLNMLSEFKLLHSEYFGWGDYSLWFQGFSIYDKTGFIMI
                     EKSQGAGNPPIRHTLEFISTNIAEFLDKLTKITDARLCKGFSDWANSVKEGASNDFKE
                     NVDIALVRLFKCVELHNSKLDLTDLHLGSLPPLPDWIEVLSLRHNGLATIQIPKFCKE
                     LELDFNNYMVFPKVSDGITQVSVDNNLISRVDSSPSKAMKIFIYRNKIW"
     gene            complement(15830..16144)
                     /gene="fliE"
                     /locus_tag="QIE41_001546"
     CDS             complement(15830..16144)
                     /gene="fliE"
                     /locus_tag="QIE41_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDS3530448.1"
                     /translation="MSAIQGIEGVISQLQTTAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            16359..18017
                     /gene="fliF"
                     /locus_tag="QIE41_001547"
     CDS             16359..18017
                     /gene="fliF"
                     /locus_tag="QIE41_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDS3530449.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            18010..19005
                     /gene="fliG"
                     /locus_tag="QIE41_001548"
     CDS             18010..19005
                     /gene="fliG"
                     /locus_tag="QIE41_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDS3530450.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            18998..19684
                     /gene="fliH"
                     /locus_tag="QIE41_001549"
     CDS             18998..19684
                     /gene="fliH"
                     /locus_tag="QIE41_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDS3530451.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            19684..21057
                     /gene="fliI"
                     /locus_tag="QIE41_001550"
     CDS             19684..21057
                     /gene="fliI"
                     /locus_tag="QIE41_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDS3530452.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            21076..21519
                     /gene="fliJ"
                     /locus_tag="QIE41_001551"
     CDS             21076..21519
                     /gene="fliJ"
                     /locus_tag="QIE41_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDS3530453.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            21516..22643
                     /gene="fliK"
                     /locus_tag="QIE41_001552"
     CDS             21516..22643
                     /gene="fliK"
                     /locus_tag="QIE41_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDS3530454.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLMATDKPTTKGEPLVSDILSDAQQADLLIPVDETPPVINDEQSTSTPLNTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTTKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAESQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQINISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            22748..23212
                     /gene="fliL"
                     /locus_tag="QIE41_001553"
     CDS             22748..23212
                     /gene="fliL"
                     /locus_tag="QIE41_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDS3530455.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            23217..24221
                     /gene="fliM"
                     /locus_tag="QIE41_001554"
     CDS             23217..24221
                     /gene="fliM"
                     /locus_tag="QIE41_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDS3530456.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            24218..24631
                     /gene="fliN"
                     /locus_tag="QIE41_001555"
     CDS             24218..24631
                     /gene="fliN"
                     /locus_tag="QIE41_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDS3530457.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            24634..24999
                     /gene="fliO"
                     /locus_tag="QIE41_001556"
     CDS             24634..24999
                     /gene="fliO"
                     /locus_tag="QIE41_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDS3530458.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQLVMKNLLKRSGRS"
     gene            24999..25736
                     /gene="fliP"
                     /locus_tag="QIE41_001557"
     CDS             24999..25736
                     /gene="fliP"
                     /locus_tag="QIE41_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDS3530459.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            25746..26015
                     /gene="fliQ"
                     /locus_tag="QIE41_001558"
     CDS             25746..26015
                     /gene="fliQ"
                     /locus_tag="QIE41_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDS3530460.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            26024..26809
                     /gene="fliR"
                     /locus_tag="QIE41_001559"
     CDS             26024..26809
                     /gene="fliR"
                     /locus_tag="QIE41_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDS3530461.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            27099..27722
                     /gene="rcsA"
                     /locus_tag="QIE41_001560"
     CDS             27099..27722
                     /gene="rcsA"
                     /locus_tag="QIE41_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDS3530462.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(27766..27954)
                     /gene="dsrB"
                     /locus_tag="QIE41_001561"
     CDS             complement(27766..27954)
                     /gene="dsrB"
                     /locus_tag="QIE41_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDS3530463.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            28117..28344
                     /gene="yodD"
                     /locus_tag="QIE41_001562"
     CDS             28117..28344
                     /gene="yodD"
                     /locus_tag="QIE41_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDS3530464.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            28642..29457
                     /locus_tag="QIE41_001563"
     CDS             28642..29457
                     /locus_tag="QIE41_001563"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDS3530465.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEEPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(29454..31148)
                     /gene="yedQ"
                     /locus_tag="QIE41_001564"
     CDS             complement(29454..31148)
                     /gene="yedQ"
                     /locus_tag="QIE41_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDS3530466.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSDNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHSTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(31319..31501)
                     /locus_tag="QIE41_001565"
     CDS             complement(31319..31501)
                     /locus_tag="QIE41_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDS3530467.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(31580..32497)
                     /locus_tag="QIE41_001566"
     CDS             complement(31580..32497)
                     /locus_tag="QIE41_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDS3530468.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            32670..33590
                     /gene="yedA"
                     /locus_tag="QIE41_001567"
     CDS             32670..33590
                     /gene="yedA"
                     /locus_tag="QIE41_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDS3530469.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(33579..34049)
                     /gene="vsr"
                     /locus_tag="QIE41_001568"
     CDS             complement(33579..34049)
                     /gene="vsr"
                     /locus_tag="QIE41_001568"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDS3530470.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     DLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPETRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(34030..35448)
                     /gene="dcm"
                     /locus_tag="QIE41_001569"
     CDS             complement(34030..35448)
                     /gene="dcm"
                     /locus_tag="QIE41_001569"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDS3530471.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDAKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AIFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADADDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(35515..36210)
                     /locus_tag="QIE41_001570"
     CDS             complement(35515..36210)
                     /locus_tag="QIE41_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDS3530472.1"
                     /translation="MDLQHWQAEFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(36250..36615)
                     /locus_tag="QIE41_001571"
     CDS             complement(36250..36615)
                     /locus_tag="QIE41_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDS3530473.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         8993 a         9542 c         9244 g         8923 t
ORIGIN      
        1 tcggggctga ttttttctta ttattgcagc agaaacttct cgaggaactg gcgggtgcga
       61 ggctgctggg gatcggcaaa taacgctttt gctgccccct gctcaactat ccgcccctgg
      121 tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg cgtcacaatc
      181 accatcgtgc gcttttcctg cgccagctga cggatggtgt tcaggacttc gcccaccagc
      241 tctggatcca gcgccgacgt tggctcgtca aacaaaatca cctcaggacg catagccagc
      301 gctcgcgcaa tcgcgacacg ctgctgttga ccgccagaca aacgacgtgg atagctggtt
      361 tctttacctg ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct
      421 tttggttcgc ctttgacgat caccggccct tcaataatgt tttccagcac cgtacgatgc
      481 ggaaacaaat taaagttctg gaagacaaac ccgacgtgct gacgcaactg gcgaatcagc
      541 gatttttgct gacttaatga acgtgcagta tcaatggtga tatcgccaac ggtgatggtt
      601 cccgcttccg gttgttccag cagatttatg ctgcgtagca acgtggtttt gccggaacca
      661 cttggaccga taattgccac tacttcgcca ggcttcacct caaggtcgat accgtgcagc
      721 accgtctgac cgtggaattt tttcaccagg ttcttaactt caatggcact cattttggtt
      781 ctctctcctg gcgattaagt tgattctcga aatggttctg caacgtcgat aacactgtcg
      841 ccatgatcca gtagatgagc gaagccgcca gatacatggt gaacacctcc agcgtacgcg
      901 aggtaatcaa ctgcgcctga cggaacagct ccggcacctg gattgtcgcg gccagcgacg
      961 tatcttttac caggctgata aagctgttcg acagcggcgg caacgccacc cgcgccgcct
     1021 gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat accaatactg gcagcagctt
     1081 cccactgacc tttatcaata gaagaaatgg cggcgcgcag cgtttcggca gcataggcgg
     1141 cagtgtttag cgacagacca atcattgctg acggaatggg atctagttcg atgccaaact
     1201 gcggcaagcc gtaatagatc ataaacagtt gtgcgatgag tggcgtacca cgaaaaatag
     1261 agatataaaa acgcgccagc cagcgcacgg gccagattgg cgaaaggcgc atcagcgcga
     1321 gaacaaaccc tagcagtaag ccaaaaaaca tgccgccgat actgagttgc agcgtatacc
     1381 cagccccttt cagcaggaac ggcaaggaat caataaccag ttgtatactt tcttgcatga
     1441 gcgtttccgg agctaaacgt ggggatgata ggcgaacagc gcaggcacgc cgccggtatg
     1501 aataaacaga atcggccctt catctttgaa gcgtttctga ctgataccgt caatcagccc
     1561 cgccatcgct tttccggtat acacaggatc aagcagaatg ccttccagcc gcgccagcaa
     1621 tttcactgct tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc
     1681 ccagagtaaa atttccgctg atgcggtcag ctccagttct ttcgcaatcg cctgttggag
     1741 gttaaccact ttcggcaatt gatcggcaac ggaacgcgac acggtcacgc caatcagttc
     1801 gctttcaggc atcaggtgtt ccagcccaac agccagtccg gcgtgagttc cggcactgcc
     1861 cgacgcgacc accaccgacg aaatattaac tgccccttca cactgttgcg caatttccag
     1921 cgcactctcc acataaccca atgcgcccaa agcattagaa ccgccaaccg gaatgacata
     1981 cgggcgaaag ccttgtgctt cgactcgcgt cgccagctct tccagttggg cattgggatc
     2041 ggtcagtgcg tcgcacattt caatctgggt attgaacaga tccagcaaca aacgattgcc
     2101 gttggttaaa tagttttctg cggttgtgcc aataggattt tccagcagcg ccacgcagtg
     2161 caggccgagt ttcgcagcga ctgcggcagt ctggcgcaca tggttagact ggatcgcccc
     2221 ggcagtaatc agcgtatcgg caccttcgcg cagagcatct gccgcgagaa attccagctt
     2281 acgtaattta ttgccgccca ttgccatggg agtgacgtca tcccgtttga tgaaaatttc
     2341 ccgtccaaga taatcagaaa agcgcggcag atattcgagc ggcgttggcg cgccgataaa
     2401 ctccagccgt ggaaaacggg ttaaattatg cagtggcata acagcctccg atgtgtgttg
     2461 ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc tagtgaaagc
     2521 gccctttttt gtcattatgc tgattatttg gtcacatcag caccaaacca tttttcggaa
     2581 agggcttgca gagtgccatc tttttgcatt tccgcaattg catcattcac cgctttcagc
     2641 aggtcttcat ttcctttacg cagcgccaca ccagactcct gacgggagaa tgcttcaccg
     2701 gttactgcca gcgtatcgtt ggttttcttc accagatcca gcgccgccag acggtcaacg
     2761 aggatcgcat cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc atcatcatag
     2821 gtacgcacat cgacgccctg aacattctga cgcagccact cttcatagtt ggtgcccaga
     2881 ccgacaccca cttttttgcc tttcagatca gcagccgttt taatggtgcc ttcgttacct
     2941 tttttcacca gcgcctgaat accggaaatg gtgtacgggg tagagaaatc gtattttttc
     3001 ttgcgctcgt cagaaatggt gacctgatta atcaccacat caatgcgttt agagtccagc
     3061 gacgccagca taccgtccca tttggtcggt tttagtgacg cctcaacgcc aagatgtttt
     3121 gccagctgtt gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc cccctgaaaa
     3181 ctgaacggcg gataagttcc ttccagccct accagcagcg tgccgcgctc tttgacttta
     3241 ttaagcagac cttcatctgc aaaactttta acgctcatgc ccgcaaccag cgccacggcc
     3301 atcacaccca tcaatgcctg acgtcccaga tgtgctaatt tcatattcac cccgaatgtt
     3361 gttatgtctg tttgcagtgt agagccattg tttgtaaaca caaaaacaac tccgctacat
     3421 cttattctta tttaatatat atcagaagct ggcagactgg aggatttctg cacaagtccg
     3481 gtacacgttt gacgctgata gtgttgatac ttccgtaacg caatgcggta attgttggtg
     3541 ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg cagctgatcg
     3601 agggaaatat tttgcccgtg tagatgatta cccaaacggc gcagacgaac gacatattca
     3661 cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa attgcttaaa gccgataata
     3721 tcgaaaaaat cactttgcgt tttgcaatgt aaatcaccgc agaatcggca caatgccgcc
     3781 catgattttt gttccacttg ccagccattt tcatcaagca gcatgtccag gcgggaaatc
     3841 tcgattttat tcactatttt gccgtcgcga acgagggtaa tgcggtcgag taatttacgg
     3901 caatgcgcgc aatgggtctg gctgtgttta aagtctttaa gatagcggct taatggccgt
     3961 cttttcaggt gctgcaccat cattaagaac tcctggtagt caaaattaaa gtgcggcatt
     4021 tactgacgtt ataacttacc cagtttagtg cgtaaccgtt taatggcctg gctgtgtaac
     4081 tgactgaccc gcgattcccc gacctccagc accgagccaa tctctttgag attcagctct
     4141 tcctggtaat agagggttaa taccagtttt tcgcgctccg gcaacgtttc gatagcttcc
     4201 atcacccgct ggcgcagatt actgtccagc agctgttgta gcgggttttc tcgctgatga
     4261 tcatcagtaa ccagttcaat gctatcgccg tgctcttcgc gccactcatc gtaggagaag
     4321 agctggctgt tattggtgtc gagcaacatt tggcgataat cggcaatatc gatccctaaa
     4381 cgttccgcta cctcagtttc cgtggcgttg cggccaagtt cctgctccag ttgccctatt
     4441 gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga
     4501 agttcatcca gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa tgccgttcct
     4561 tgtagggcgt catagcgttc gacggcatta agtaacccaa tgccgcccgc ctgtagcaga
     4621 tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc
     4681 agcgggacat aacgctgcca cagcgagtgt ttatccatta caccttcagc ggtatagagt
     4741 gaattcacga taaacagccc tgcgttatat gagttatcgg catgattatc cgtttctgca
     4801 gggtttttaa tcggacgatt agtgggtgaa atgaggggtt atttgggggt taccggtaaa
     4861 ttgcggacag aaaaaacccc gccggtggcg gggaagtacg ttgctgacaa gttaccattc
     4921 atgttgccgg atgtggcgta aacgccttat ccggcctaca aaaatgtgca aattcaataa
     4981 attgcaattc accttgtagg cctgataagc gcagcgcatc aggcaatttt gcgttgccgt
     5041 cagtctcagt taatcaggtt acgacgatta accctgcagc agagacagaa cctgctgcgg
     5101 tacctggtta gcttttgcca gcacggagtt accggcctgc tggatgatct gcgctttcga
     5161 catgttggac acttcggtcg catagtcggc gtcctgaata cgggactgcg cttcggacag
     5221 gttggtagtg gtgttgttca ggttggtgac cgcggaatcc agacggttct ggatagcacc
     5281 cagggatgaa cggaacttat cgatctggct gattgcgtca tccagagcgg ccagcggatc
     5341 ggtggtggtt gcggctttgg ttgcagcgtc ggtagtgaaa tcaccatccg cttctacata
     5401 gaccgcttta cctgaaccgt tggttacaga gccatctttc tgcagataca gtttagtggt
     5461 gttatcagta gtcagggaac catctttgct ggtgtaggac tgagcagtag tcccgccgat
     5521 agtcacttta ccatcagtac ctacggtcag acctgtagcg ccagtaccaa cagtaaagcc
     5581 cgtagattta gttgctgact gcagagaatc agcactgata cgaacaccgg tcagaccaat
     5641 attaccgtta gcagcaatat caatattagt gccatcttca gtagtgatgg ttgaagcaac
     5701 actagagctc tgaaggccgg tcagagtagc cagatcagca gaggtcaagc cagcactgtt
     5761 ctgagtcagg ttacctgtat tgtcgatata taatgcagaa ccatcttttg ctgtaatctt
     5821 accatcactg gccagaacga catcaaccga tgtcgtacca acggttactt tcagatttgc
     5881 agtatcacct gctttcggcg tcaggaagga ctgaacctgg gctgcagtat tattggcgct
     5941 tgaagctacg ttgtaagtga agtcgccttt tactgagtca taagtatagt cattggaagt
     6001 cagcgcactg gaatcaccga agccattatt aatggttgca gtaattgcag aagtcggagc
     6061 actgccgtct ttgatgctag caataacatc tgcagcagtg gattctttat aaccagcact
     6121 gacagtataa gtaactgtac catttgcgtc tgctgcactg ggtgcagaga gttgagcagc
     6181 ggttaaatca gctttagttg ctgcagtatt cgccacagaa ccagaaccgt taacgttaaa
     6241 acctgtcagg ttcaaggtag aagagtcaat cttcttcagg tcgatagtga tggtctggcc
     6301 atcgttcgcg ccaacctgaa ttttcatgga accgtctttt gccagcacgt tcacgccgtt
     6361 gaactgggtc tgaccggata cgcggtcaat ttcgtccaga cgggatttaa tttcgtcctg
     6421 gatagagtcc aggtcagact gggagttggt accggtggtc gcctgaacgg tcagttcacg
     6481 cacacgctgt aagttgttgt tgatttcgga cagcgcgcct tcggtggtct gtgcaacaga
     6541 aataccgtcg ttggcgttac gtgcagcctg agtcaggcct ttaatattag aagtaaaacg
     6601 gttagcaatc gcctgacctg cggcgtcatc cttcgcgctg ttaatacgca agccagaaga
     6661 cagacgctcg atagaactcg acagcgcaga ctggttcttg ttgatattat tttgagtgat
     6721 cagcgagagg ctgttggtat taatgacttg tgccatgatt cgttatccta tattgcaagt
     6781 cgttgattac gtattgggtt tccacccgtc ggctcaatcg ccgtcaaccc tgttatcgtc
     6841 tgtcgtaaaa caacctttag aatttttttc acaaacagcc attttttgtt agtcgccgaa
     6901 atactctttt ctctgcccct tattcccgct attaaaaaaa acaattaaac gtaaactttg
     6961 cgcaattcag gccgataacc ccggtattcg ttttacgtgt cgaaagataa aaggaaatcg
     7021 catggcaagt atttcatcgc tgggagtcgg gtcaggtctg gatttaagtt caatccttga
     7081 tagcctcacc gccgcgcaaa aagcgacgct aacccccatt tcaaatcagc aatcgtcgtt
     7141 taccgctaaa cttagcgcct acggtacgct gaaaagcgcg ctgacgactt tccagaccgc
     7201 caatactgca ttgtctaaag ccgatctttt ttccgccacc agcaccacca gcagcaccac
     7261 cgcattcagt gccaccactg cgggtaacgc catcgccggg aaatacacca tcagtgtcac
     7321 ccatctggcg caggcgcaaa ccctgaccac gcgcaccacc agagacgata cgaaaacggc
     7381 gatcgccacc agcgacagca aactcaccat tcaacaaggc ggcgacaaag agccgatcac
     7441 cattgatatc agcgcggcta actcgtcgtt aagcgggatc cgtgatgcca tcaacaacgc
     7501 aaaagcaggt gtaagcgcaa gcatcattaa cgtgggtaac ggtgaatatc gtctgtcagt
     7561 cacatcgaat gacaccggcc ttgataatgc gatgacgctc tcggttagcg gtgatgatgc
     7621 gctacaaagt tttatgggct atgacgccag tgccagcagc aacggcatgg aggtctcggt
     7681 cgccgcccag aatgcgcagc tgacggtcaa caacgtcgcc attgagaaca gcagcaacac
     7741 catcagcgac gcgctggaaa acatcaccct gaacctgaac gatgtcacca cgggcaacca
     7801 gacgctaacc atcactcagg acacctccaa agcgcaaacg gcgattaaag actgggtgaa
     7861 tgcctataac tcgctaatag ataccttcag cagcctgacc aaatacaccg ccgtagatgc
     7921 gggagctgat agccagagtt ctagcaatgg cgcactgctc ggcgactcca cgttgcggac
     7981 gattcagacg cagttgaaat cgatgctgag taataccgtt agttcttcca actataaaac
     8041 gttggcgcag attggtatca cgaccgatcc cagcgatggc aaactggaac tggatgccga
     8101 caaactcacc gctgcactga aaaaagatgc cagcggcgta ggtgcattga ttgttggcga
     8161 tggtaaaaaa accggcatca caaccaccat cggcagcaac ctgaccagtt ggctttcgac
     8221 aacgggcatt attaaagccg ctaccgatgg cgttagtaag accctgaata agttaactaa
     8281 agactacaac gccgccagcg atcgcattga tgcgcaggtc gctcgctaca aagaacaatt
     8341 tacccaactg gacgttttaa tgacctcgtt aaatagcacc agcagctact taacgcagca
     8401 gttcgaaaac aacagtaatt ccaagtaatc attatttatc gggagacagg tcatgtacgc
     8461 ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag
     8521 ccagcagcag ttggtcacca tgctatttga tggagtgctg agcgcactgg ttagagcgcg
     8581 cctgtttatg caggacaaca atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa
     8641 catcattgag aacgggctgc gagtgagtct tgatgaagag agcaaagacg aactaaccca
     8701 aaacttgatt gctctttata gctatatggt caggcgcttg ctgcaagcca atttacgcaa
     8761 cgatgtctcc gcagtcgaag aagtggaagc attaatgcgc aatattgccg atgcctggaa
     8821 agagtcttta ctctcccctt ctttgattca ggacccagtc tgatgaacca tgcaccgcat
     8881 ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc tcatgttacg cctggcaaca
     8941 gaagaacagt gggacgaact catcgccagc gaaatggcgt atgtgaatgc ggtgcaggag
     9001 attgcacacc tgactgaaga ggttgagccg tccaccacga tgcaggagca actccgcccg
     9061 atgctgcgcc tgattctcga caacgaaagc aaggtaaagc agttattaca gattcggatg
     9121 gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat
     9181 ggtgatcagg gcggctttgt gctggctccg caggataacc tcttttgaat cttaacgagc
     9241 cgatggctcg cgaataatcc gattacggct acgcttctaa tgttcccctt gaatggagtc
     9301 gaagaatgcg taatcccacg ctgttacaat gttttcactg gtattacccg gaaggcggta
     9361 agctctggcc tgaactggcc gagcgcgccg acggttttaa tgatattggt atcaatatgg
     9421 tctggttgcc gcccgcctat aaaggcgcat cgggcgggta ttcggtcggc tacgactcct
     9481 atgatttatt tgatttaggc gagtttgatc agaaaggcag catccctact aaatatggcg
     9541 ataaagcaca actgctggcc gccattgacg ccctgaaacg taatgacatt gcggtgctgc
     9601 tggacgtggt agtcaaccac aaaatggggg cggatgaaaa agaagctatt cgcgtgcagc
     9661 gcgttaatgc tgacgaccgt acgcaaattg atgaagaaat cattgaatgc gaaggctgga
     9721 cgcgttacac cttccccgcg cgtgccgggc aatactcgca gtttatctgg gatttcaaat
     9781 gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga tggcattttt aaaattgtta
     9841 acgactacac cggcgaaggc tggaacgatc aggttgatga tgaactcggt aatttcgatt
     9901 atctgatggg cgagaatatc gattttcgca atcatgccgt gacggaagag attaaatact
     9961 gggcgcgctg ggtgatggaa caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac
    10021 atattccggc gtggttttat aaagagtgga tcgaacacgt acaagaagtt gcaccaaagc
    10081 cgctatttat tgtggctgaa tactggtcgc atgaagtcga taaactgcaa acgtatattg
    10141 atcaggtgga aggcaaaacc atgctgttcg atgcaccgct gcagatgaaa ttccatgaag
    10201 catcgcgcat ggggcgcgac tacgacatga cgcagatttt cacgggtaca ttagtggaag
    10261 ccgatccttt tcacgccgta accctcgttg ccaaccacga cacccaaccg ttgcaagccc
    10321 tcgaagcccc cgtcgaaccg tggtttaaac cgctggcgta tgctctaatt ttgttgcggg
    10381 aaaatggcgt tccttcggtg ttctatcctg acctctatgg cgcgcattac gaagatgttg
    10441 gcggtgacgg gcaaacctat ccgatagata tgccaataat cgaacagctt gatgagttaa
    10501 ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt atttttcgac catccgaact
    10561 gcattgcctt tagccgcagt ggcactgacg aatatcccgg ctgcgtggtg gtcatgtcga
    10621 acggggatga tggcgaaaaa accattcatc tgggagagaa ttacggcaat aaaacctggc
    10681 gtgattttct ggggaatcgg caagagagcg tagtgaccga cgaaaacggc gaagcgacct
    10741 tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga agaggtgatt taaattcatc
    10801 cccggcggca agccggggag atttcattac ggcagtggcg tcggtaacgg cgctttatcc
    10861 agcgcggcgg cacactctgc cgtcgggcga tccacacgct ccatcgtcat accatcgtac
    10921 tcaatggtgt taccgtcacg ctcaacctcg tacagctcac gttttacggt aacgttagtg
    10981 agatcatctg acatcaatgt caacttgccc ggcacggcaa tcacccgctg ccactgacgg
    11041 caatccagag tatcgccctc tttagtgaca atcaggctgc ctatcgcttc cggactgacc
    11101 agcgcacgtt gcggcccttt ggtctgccag tagcccgcca gccagtccgg cgcgggggtt
    11161 ttcacgacat tgttatagtt ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct
    11221 gcaattgcga gttttttcat cattcgtcct gcatgagaag aaaagaggat tgtggcatta
    11281 aagccctgat gtcgccagcc tttccaggtt cagagagaaa tttgatctaa ctattatttt
    11341 tgtgaattat taccccgaaa ataaatttgc atggtgtatg attcgcgact cctgtttcaa
    11401 accttctgag ttcagaggct acattcatgt catggcagca attcaaacac gcctggttga
    11461 ttaaattctg ggcgcccatc cctgcggtca tcgcggcggg tattctctcc acttactatt
    11521 ttggcatcac tggcaccttt tgggctgtca cgggtgaatt tacccgttgg ggcggtcagc
    11581 tcctgcagct gttcggcgtc catgctgaag agtggggtta ttttaaaatt atccatctgg
    11641 aaggatcgcc attaacccgc atcgacggga tgatgatcct cggtatgttt ggcggctgct
    11701 ttgctgcagc gctgtgggcc aacaatgtca aactacgcat gccacgcagc cgtatccgca
    11761 ttatgcaagc cattattggc ggcattatcg ctggttttgg cgcgcgtctg gcaatgggct
    11821 gtaacctggc ggcgttcttt accggcattc ctcagttctc gctgcatgcc tggttctttg
    11881 ccatcgccac tgccattggt tcatggtttg gcgcgcgctt taccctgctg cccatcttcc
    11941 gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc
    12001 aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt cggcatgctg ggctgggcac
    12061 tgctgacggc gatgaaccaa ccaaaactgg ggctggcaat gctgtttggc gtcggctttg
    12121 gtttactgat tgaacgtgcg caaatctgct ttacttcggc gttccgcgat atgtggatca
    12181 ccggacgtac ccatatggcg aaagcaatca ttatcggtat ggcggtgagt gccatcggga
    12241 tcttcagtta cgtgcagtta ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg
    12301 taattggtgg tttactgttt ggttttggca tcgtgctggc aggcggctgc gaaaccggct
    12361 ggatgtaccg cgcggtagaa ggccaggtgc actactggtg ggtcggtctg ggcaacgtga
    12421 tcggctcaac gattctggcg tattactggg atgatttcgc tccggcgctg gccaccgact
    12481 gggacaaaat caacctactg aaaacctttg gtccgatggg tggcctgctg gtgacatatt
    12541 tgctgttgtt tgctgcgctg atgttgatta tcggctggga aaaacgcttc ttccgccgtg
    12601 cggcaccgca gactgctaag gagatcgcat gaaaaatatc gttcctgatt accgccttga
    12661 tatggtgggt gaaccctgcc cttatccggc agtcgcaacc cttgaggcga tgccgcaatt
    12721 aaaaaaaggt gagatcctgg aagttgtgag cgactgtccg cagtcgatca ataatattcc
    12781 actggatgcg cgtaatcacg gctatacggt gctggatatt cagcaagacg ggccgaccat
    12841 tcgttattta attcagaagt aatccctcat tcccgctgtg gcccacagcg ggaacatcgt
    12901 tgcctctgac acatagattt tacccgcccc ctctcctatt cttttcagta tgatctcagg
    12961 aggttgctat gtgtggacgc tttgcccaat cccaaacgcg tgaagattac cttgcgcttc
    13021 tcgcggaaga tattgaacgc gatattccct acgatcccga acccattggc agatacaacg
    13081 tcgcgccggg aaccaaagtt cttctactca gtgaacgtga tgaacacctt catctggatc
    13141 cggttttttg gggatatgct cccggatggt gggataaacc gccgctgatt aacgcccgcg
    13201 tagaaactgc cgcaaccagt cgcatgttta aaccgctctg gcaacatggt cgggcaatct
    13261 gttttgtcga tggctggttt gagtggaaaa aagaaggcga caaaaagcag ccttatttta
    13321 tctatcgtgc tgatggacaa cctgtttttc tggccgcgat aggtagcaca ccgttcgagc
    13381 gcggtgacga agccgaagga tttttgattg tcactgcagc agcagaccag ggtctggtag
    13441 atattcatga ccgccgccca ctggtactgt cgccagaagc tgcgcgggaa tggatgcggc
    13501 aagatattgg cggtaaagaa gcctcagaaa tcgctaccag tggctgtgtg ccagcaaacc
    13561 agttcacctg gcacccggta tcgcgcgcgg tgggtaatgt caaaaaccag ggcgcggcgt
    13621 taattcaacc tgtttgacaa gcctgaagat tattgatcaa caatactgcg tcataagaaa
    13681 tctctattag acaaagattt cattacctgt tggcatattg caaaaataac accaatgcgg
    13741 aatcgttatg ttcacgatta aaacagatga tctcacccat ccagcagtgc aagcattagt
    13801 ggcttaccat atttccggca tgctgcagca gtctcccccc gaaagcagtc atgctttaga
    13861 tgtgcaaaaa ttacgtaacc cgacagtgac attctggtca gtatgggaag gcgaacaact
    13921 cgcaggaatt ggtgcgctga agttgctgga tgataaacat ggcgaactga aatcaatgcg
    13981 taccgcgcca aattatttac gtcgaggtgt cgccagtctg attttacgcc acattttgca
    14041 ggtcgcccat gacagatgcc ttcatcgcct gagcttagaa acgggtacac aggctggatt
    14101 tacggcctgc catcaacttt atttgaagca tggtttcgtt gattgcgaac cgtttgctga
    14161 ttatcaactt gatccacaca gtcgattttt gtcattgacg ctatgcgaaa ataatgaatt
    14221 gccatgagcc agacgcagca cattcttgca ttcgacgtgc tgcgccttta ttcatcacca
    14281 gccgcatatg tcttgtcctt agacgctcct tccacatgcg ccacaagaaa cctctattcc
    14341 agtgacacaa ttgcgcctaa ctatttaatt cttaccatct attacgcccc ttttgactat
    14401 gtcagaatat ttatcataat cagcggaatt cgtaacaata tattttcgct gcgatatttc
    14461 ataggtaatg tctgtcaaag tccacggcat taaaaactcc tctttaatag gataacattc
    14521 tataaaaagt aattttaaat tgctatgtat cgcagggaca acctcaatat tatactccat
    14581 acgtgctgtc tccagatgat ttgggaagta ttgaattttt ttatcttatt atcgccacaa
    14641 ttaaacagat tagtagtctc aggcattgct ggtatttacc atattttatt gcgataaata
    14701 aaaattttca tagcctttga cggagatgag tcaacacgcg atatcagatt attatctacg
    14761 gagacctggg taattccatc ggatactttt ggaaatacca tataattatt aaaatcgagt
    14821 tctaattctt tgcagaattt gggaatttgt attgtggcaa ggccattatg acgaagagaa
    14881 agcacctcaa tccagtctgg aagaggtggc aaagacccca agtgaagatc tgttaagtca
    14941 agtttactat tgtggagttc aacacattta aacaatctca ccaatgctat atccacattt
    15001 tctttgaagt cattcgatgc gccttctttg actgaattag cccagtcact gaaacctttg
    15061 caaagtctgg catctgtaat tttcgttaac ttgtctagaa actctgcaat attagtcgat
    15121 ataaactcta atgtatgccg aatgggtgga ttaccagctc cctgactttt ttctatcatg
    15181 ataaaacctg tcttatcata aatcgagaaa ccttgaaacc agagactata atcaccccat
    15241 ccgaaatatt ctgagtgcaa caacttaaat tctgacagca tattcagtaa tttgctgtct
    15301 tcactaattg gtgtaaacct accatttcca agacaactga ctaactcact tgataattca
    15361 tttatcattg catcatcaga tactaaatct tgttgtttta tcgtagactc agtattagat
    15421 gtgattttat ttaattgaac cgactgagct tttaacgaca gatcatttaa tggaaacatt
    15481 tacactcctg tttaataaat tatattgccc agggagcatt ctaaaacaca ataaaattac
    15541 cactatcaaa aatcgctcat ttttataacg cagtatatta ataaaaataa gaacgaagat
    15601 taaattatcc agaggttact gttgcgtctg tggtcgtaac aacatccgac gtggtgatga
    15661 gtgtcaaatg agacactctg atggtcaaag ggtttattgc gtttacggat cgtggatggc
    15721 agaaaacgtt caggatcagg tatccatttt aaaccagaaa tagagtgagt ttgccccatc
    15781 catgccccac gcggtgagat cggatgtaat taacaacagg ttacacaacc tacacctgca
    15841 tgctcatcac ttcctgatac gccgccacca gcttattacg cacctgaatc cccatttgca
    15901 ttgaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg ccgggttcac
    15961 cgagagtgaa tttttctgcc tgcgtgcggg cggctgtttg tgtatcgctt atgcgatcga
    16021 gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg cggcagtgat tcctgcgcac
    16081 gtgcactcat cgccgtggtc tgtaactggc tgataacccc ttcaatcccc tgtatcgctg
    16141 acattttcat ctcccggata atttctggta gcaaagccta ccagtaagtc aataagacaa
    16201 aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca
    16261 aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt
    16321 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
    16381 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg
    16441 ccggttccgc agcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
    16501 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
    16561 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa gttccggcag
    16621 ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa ggcggcgcgg
    16681 tcggtttcga actgctcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga
    16741 attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa
    16801 aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat
    16861 ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa
    16921 ttagcgccat tgtgcatctg gtttccagcg cggttgctgg tctgccgccg gggaacgtca
    16981 cgctggtgga tcagggtgga catctgttaa cccagtccaa taccagcggg cgcgatctta
    17041 atgacgctca gttgaaatat gccagcgatg tcgaaggccg gattcagcgg cgtattgaag
    17101 cgatcctgtc gccaattgtt ggtaacggta atattcacgc ccaggttacg gcgcaactgg
    17161 acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcagg
    17221 cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg
    17281 gcgtaccggg ggcgttgtcg aatcaaccgg ctcctgcgaa taacgcgcca atcagcacgc
    17341 ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat agtgggccgc
    17401 gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
    17461 aaatgaacgt gggcgatgtg caacgtctgt ccgtcgcggt cgtggtgaat tacaaaacct
    17521 tgccagatgg caaaccgttg cctctcagca acgaacagat gaagcaaatt gaagatctga
    17581 cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc
    17641 cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag caagcgttta
    17701 tcgatcagtt acttgctgcc ggtcgctggt tgctggtact gctggtggcg tggctgctgt
    17761 ggcgtaaagc ggtacgcccg cagctaacac gtcgcgctga ggcgatgaaa gctgtacagc
    17821 aacaggcaca ggcccgcgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg
    17881 aacaactcca acaacggcgc gctaaccaac gtctgggggc agaagtcatg agccagcgta
    17941 tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa
    18001 ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctact gatgaccatt
    18061 ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaactctg
    18121 agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccga tgtgctggcg
    18181 gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg
    18241 cgttcggtat tggtcaaagc tctgggtgaa gaacgtgccg ccagcctgct ggaagatatt
    18301 ctcgaaaccc gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagc
    18361 gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg
    18421 aagcgcgccc aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg
    18481 atgttgcgta tcgccacctt tggcggcgtg cagcctgccg cgctggcgga gctgaccgaa
    18541 gttctgaatg gcttgctcga cggtcagaat ctcaagcgca gtaaaatggg cggcgtgaga
    18601 acagcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt tattaccgcc
    18661 gtgcgtgaat tcgacggcga gctggcgcag aaaatcatcg acgagatgtt cctgttcgag
    18721 aatctggtgg atgtcgacga tcgcagcatt cagcgactgt tgcaggaagt ggattccgaa
    18781 tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat
    18841 atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt
    18901 ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg ccttgccgaa
    18961 actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa
    19021 aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca tggtcgagcc
    19081 ggaagaaacc atcattgaag aggccgaacc cagccttgag cagcaactgg cgcaactgca
    19141 aatgcaagcc catgaacaag gttatcaggc gggtattgcc gaaggtcgcc agcaaggtca
    19201 cgaacagggc tatcaggaag gactggccca ggggctggag caaggtctgg cagaggcgaa
    19261 gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac
    19321 ccttgatgca cttgatagtg tgatagcgtc gcgcctgatg cagatggcgc tggaggcggc
    19381 acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca aacagatcca
    19441 acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg tgcacccgga
    19501 tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg gctggcgctt
    19561 gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct
    19621 cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt
    19681 gtaatgacca cgcgtctgac tcgctggcta accacgctgg ataactttga agccaaaatg
    19741 gcgcagttgc ctgcggtacg tcgctacggg cgattaaccc gcgctaccgg gctggtgctg
    19801 gaagccaccg gattacaatt gccgctcggc gcaacctgtg tgattgagcg ccagaacggc
    19861 agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg gctgttttta
    19921 atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gtgtttatgc caaaaacatt
    19981 tcggctgaag ggctgcaaag cggcaagcag ttgccgctcg gtccggcgtt attaggtcgc
    20041 gttctggacg gcagcggtaa accgctcgat ggcctcccct cccccgatac gacggaaacc
    20101 ggtgcgctga ttaccccgcc atttaaccca ttgcaacgta caccgattga acatgtgctg
    20161 gacaccggcg tgcgcccaat caatgccctg cttaccgtcg ggcgcgggca gcgtatgggg
    20221 ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc ccgttacacc
    20281 cgcgctgatg tcattgtcgt gggtttgatt ggtgaacgag ggcgcgaagt aaaagatttt
    20341 attgagaata tcctcggtgc cgaagggcgt gcacgctccg tggtgattgc cgctccggcg
    20401 gatgtttctc cgctcctgcg aatgcagggt gccgcctatg ccacgcgcat tgccgaagat
    20461 tttcgcgatc gtggtcagca tgttttgctg attatggact ccctcacccg ctacgcgatg
    20521 gcccagcgtg agattgcgct ggcgattggc gaaccacctg ccactaaagg ttatccaccg
    20581 tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggtat tagcggcggc
    20641 ggctcgatta ccgcgtttta taccgtgctc accgaaggcg atgaccagca ggaccccatt
    20701 gctgactccg cgcgggcaat cctcgacggt cacattgtgc tgtctcgccg actggcggaa
    20761 gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat gacggcgttg
    20821 atcagcgagc aacattatgc gcgagtgcgc accttcaaac agctgttgtc gagttttcaa
    20881 cgtaaccgcg atctggttag cgtcggtgcg tatgccaaag gcagcgatcc gatgctcgat
    20941 aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat ttttgaacgc
    21001 gcggactggg aagcatctct ccaggggctg gagcgtattt tcccgacagt gtcataaccc
    21061 aggagataac ggcagatggc agaacatggt gcgctggcga ccctgaaaga tctggcagaa
    21121 aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcggatg tcagcaggcg
    21181 gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaatagc
    21241 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
    21301 ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac
    21361 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag
    21421 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
    21481 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcatagcg cccttgatta
    21541 ccgctgacgt tgataacgcc acattgcctg gcggcaaagc cagcgatgct gcacaagatt
    21601 ttctcgcgtt gttgagcgaa gcattaacag gcgaggcaac aaccgacaaa gcgactcccc
    21661 agttgctgat ggcaacagat aagcccacga caaaaggcga gccgctggtc agcgatattc
    21721 tttcagacgc gcaacaagcg gatttactga tccctgtgga tgaaacaccg cctgtcatca
    21781 acgacgaaca atccacatca acaccgttaa acaccgctca gacgataacg ttggctgcgg
    21841 cggctgacaa caatacggca aaagacgaaa aagcggatga tctgaatgaa gacgtcaccg
    21901 ccagcctgag tgcccttttt gcgatgttgc cgggttttga caatacgacc aaagtgactg
    21961 atgcaccgtc aaccgtgtta ccggcagaga aaccaacgct tttcacaaaa ctgacttctg
    22021 cgcaactcac aacagcacag cctgatgacg cccccggcac gccagctcag ccattaacac
    22081 cgctggtagc agaatcccag agtaaagcgg aagtcatcag cacgccttcg tcggtgaccg
    22141 ctgccgccag cccgctaatc actccacacc agacacagcc actgcccacc gtcgccgcgc
    22201 ctgttttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt
    22261 cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcaccca caggatttag
    22321 gtgaagtgca aatttccctc aaagtggatg ataaccaggc gcaaatccag atggtttcac
    22381 cgcatcaaca cgtacgcgcc gcgctggaag cagcgctgcc ggtactgcgt acgcagctgg
    22441 cggaaagtgg cattcagtta gggcaaatca acatcagtgg cgaaagcttt agtggtcagc
    22501 agcaggccgc ttcccaacaa cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg
    22561 gggaagacga cgatacgctt ccggttcccg tctctttaca agggcgtgta acaggcaaca
    22621 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
    22681 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
    22741 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat
    22801 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
    22861 tcaggttgcc gctgaagaca aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc
    22921 gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata tcggcataac
    22981 cctgcgactg aaagatgaag ctacccgctc gcggctgagt gagtatttgc cggaagtccg
    23041 tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga cagaagaagg
    23101 caagaaaaac ctgattgccg agattaaaac cacactttcc accccgcttg ttgccgggca
    23161 accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg
    23221 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggcgac agcgaagtca
    23281 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
    23341 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
    23401 cccgccattt tcgtatgggg ctgttcaacc tgctgcgccg tagcccggac attaccgtcg
    23461 gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc
    23521 tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttc tcaccgagtc
    23581 tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg
    23641 aaggtcgcga gtttacccat acagaacagc gcgtcatcaa ccgcatgtta aagctggcgc
    23701 ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag tacgtgcgtt
    23761 cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatc gtggttaaca
    23821 cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatttgc ctgccattca
    23881 gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
    23941 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctcgagctgg
    24001 tcgccaactt tgccgatata tcgctacgcc tgtcgcaaat tttaaaactg aaaccaggtg
    24061 acgtcctgcc gatagaaaaa cccgaccgca tcatcgccca tgttgacggc gtcccggtgc
    24121 tgaccagtca gtacggcacc ctcaacggtc agtatgcgtt acggatagaa catttgatta
    24181 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc
    24241 cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg aacaaaaatc
    24301 aaccagtagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
    24361 cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga ccgtcgaact
    24421 gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc
    24481 gctggacggt ctggcgggcg aaccgctgga tattctgatc aacggttatt taatcgccca
    24541 aggcgaagtt gtcgtagtcg ctgataaata tggcgtgcgg atcaccgata tcattactcc
    24601 gtctgagcgg atgcgccgcc tgagccgtta gtgatgaata accacgctac tgtgcaatct
    24661 tccgcgccgg tttctgctgc gccactgctg caggtgagcg gcgcactcat cgccattatt
    24721 gccctgatcc tcgctgctgc ctggctggta aaacggctgg gatttgcccc gaaacgcact
    24781 ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg
    24841 gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
    24901 ctgcataaac ttcccccttc agcaccaacg gaagagatac cgcagaccga ttttcagttg
    24961 gtcatgaaaa acttgcttaa gcgtagcggg agatcctgat gcgtcgtttg ttgtctgtcg
    25021 cacctgtcct tctctggctg gttacgcccc tcgcctttgc gcaactgccg ggtatcacca
    25081 gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca
    25141 ttacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc acccgcatca
    25201 tcattgtttt tggtttattg cgtaacgcac tgggaacgcc ctccgcgcca cctaaccagg
    25261 tattgctggg gctggcactg tttttgacct tttttattat gtcgccggtg atcgacaaaa
    25321 tttatgtcga tgcgtaccag ccatttagcg aagagaaaat atcaatgcag gaggcgctgg
    25381 aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgtgag gcggatttag
    25441 ggttgtttgc cagactggcg aatactggcc cgttgcaggg acctgaagct gtgccgatgc
    25501 gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca
    25561 cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg
    25621 ggatgatgat ggttccccca gccaccattg ctctgccctt taaactgatg ctgtttgtac
    25681 tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag
    25741 gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg
    25801 cactggctgc cccactgttg ctggtggcgc tggtcacggg tcttatcatc agtattttgc
    25861 aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc atcgccgtat
    25921 ttatcgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    25981 ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc aggtaacaag
    26041 cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct
    26101 catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct
    26161 ggcaatgatg atcacgttcg ctattgcccc atcattacct gccaacgatg ttcctgtttt
    26221 ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg cgcttggttt
    26281 taccatgcaa tttgcctttg ccgctgtgcg aaccgctggt gaaattatcg gtctgcaaat
    26341 ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc ccgttttagc
    26401 gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc atttatggtt
    26461 gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac cgttgaacag
    26521 caatgcgttt ctggcactca ccaaagcagg gagtttgatt ttccttaacg gactgatgct
    26581 ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt tactgaatcg
    26641 tatggccccg caattatcca tttttgttat tggatttcca ttaactctga ctgtcggtat
    26701 ctctttaatg gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga
    26761 gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat tccgtaacgt
    26821 ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct tccaggatac
    26881 tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta ctttacttta
    26941 agacaattcc aggcaaatta tacaacactt tacgggatag taagtccgcc tgaaaaatcg
    27001 cgagagtggc gcattaggtg acccatgttg ttccgtttag tcatgatgaa atattcaggt
    27061 aaggggaatt atcgttacgc attgagtgag ggtatgccat gtcaacgatt attatggatt
    27121 tatgtagtta cacccgacta ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa
    27181 gagaaatcaa cgacattgaa accgttgatg accttgccat agcttgtgat tcacagcgcc
    27241 cttcagtggt gtttattaat gaggactgtt tcatccacga tgcttctaac agtcagcgca
    27301 tcaagcacat cattaatcaa catcccaata cgttatttat cgtttttatg gcaattgcca
    27361 atgttcattt tgatgaatat ctattggtca gaaaaaattt attgatcagt tctaaatcga
    27421 ttaaaccgga atctctcgac gatatccttg gcgatattct gaaaaaagag acaacgataa
    27481 cctcgttttt aaatatgccg acgttatcat tgagccgaac cgaatcgagt atgttgcgaa
    27541 tgtggatggc aggtcaggga accattcaaa tctccgacca aatgaatatc aaagccaaga
    27601 ccgtttcatc gcataaaggt aatattaaac gtaagatcaa aacgcataat aaacaggtta
    27661 tctaccatgt cgtccgactg acggataatg tgactaatgg tatttttgtc aacatgcgct
    27721 aacacattct gactggtggt ttcccaccag tcaggctgaa ttagattact ctgctttctc
    27781 cacaaagata ccgtcctgat gccctgcttc attaaagaac cagatgccga gcgggtagtc
    27841 ttccagcgaa accaggtaca ttgtgccttc actaaactcc tcaactgcca gtaccacgcc
    27901 aggacgacgc ggaccgccat ccgttttgac tgttacccga tcattcacct tcatcgtttt
    27961 cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt tctggcagcg ccaggcgcgc
    28021 gcgagtgctg attttctcga cggtctatac ttaagagatg ccaggcggac ttaacgactg
    28081 gcggcaacaa cagagtaacg gttgcgagga aagatgatga aaaccgcaaa agagtacagc
    28141 gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa
    28201 attagcgaaa gcgaagttca tcgcagccag aacgattctg aacacgttag cgtcgatgga
    28261 cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat tcatgacttc
    28321 agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct gagggggtcg
    28381 ggatgaaact tgcttaagca agaagcactt aaaaaattcg ttacaccagg aaatctgatg
    28441 tgttcatcac cttatccgca atttttttcg ctgacaagaa aatattcgtg atatgaatga
    28501 ttaacgctac ttatgaatag aaatgtgacg cgcgtcaccc cttggtggct ctggcgtcaa
    28561 cgggatcgct cccgaaaaaa acatctttga taatttgtgc tgttttgaga atcatcctgg
    28621 taaggggagt actgcgcaac catgctttca attcaacaac cactactggt ttttagcgat
    28681 cttgatggca ccctgctgga cagtcatagt tatgactggc aaccagctgc cccctggctc
    28741 agccgtttac gcgaagcgaa tgttcccgtc attctctgta gcagtaagac atcagcggaa
    28801 atgctgtact tgcaaaaaac gttggggcta caaggtttac cgctgattgc cgaaaatggc
    28861 gcagtgatcc agcttgctga gcaatggcag gatatagacg gttttccacg catcatctca
    28921 ggtattagcc atggcgaaat cagccaggtt ttaaatacgc tacgcgagaa agaacatttt
    28981 aaattcacaa cttttgatga tgtcgacgat gcaaccatcg ccgaatggac gggattaagc
    29041 cgtagccagg cggcgctgac gcagctacat gaggcgtcgg taacgctaat ctggcgcgac
    29101 agtgacgagc gtatggcaca atttaccgct cgtctgaacg aactgggctt gcagtttatg
    29161 cagggtgcgc gcttctggca cgtcctggat gcctctgccg gaaaagatca ggctgccaac
    29221 tggattatcg cgacctatca acaattgtca ggcaaacgcc caaccacact tggcctgggc
    29281 gatgggccaa acgatgcgcc cttactggag gtaatggatt acgcggtgat tgtgaaaggg
    29341 ctaaaccgtg aaggggtgca tctgcatgat gaggagccgg cccgcgtctg gcgaacgcag
    29401 cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt ttttctccgc ccattaagcg
    29461 ttatcgctcg cgaatacccg attgcgcccg gcctgtttag cgagataaag tcgacggtcg
    29521 gccagtgact ggagttgttc aaaatcataa tcaccggttt cctcgctgct gcttaccccc
    29581 agcgaggcac tgatgcgtat cgtcgtactc ttggcgatca acatctcttt ttcatttagc
    29641 ttcaggcgaa tacgttctgc aacctccgca gcctgcgtca ggctcgcgcc gggcagaatc
    29701 acacaaaact cctcaccacc gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta
    29761 attaatccgg cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta
    29821 atcgctttaa aatggtcaag gtcgacctga atgacagaaa aaggatgttg gtgcgtctga
    29881 cacaatttag cgagcggacg ggctttttcg aacagtgcgc cacggttata taaacgcgtt
    29941 aaggtgtcgt gccacgcctg ccactgcaac gagctttgca aaacatacat gttgctgacc
    30001 atccggcgaa tcacatacca ggagatgagt aacatggtgg taaagagcgc ccacagcagg
    30061 gttaatgcaa tgctgatact gccgaaatcg ccgcgcacgc cttcgcttag cgtatggacg
    30121 cgcaccagca caccgtcgaa atggtccaga cgttcccagc taacatagcg actgttcata
    30181 cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag ttcacgaggg
    30241 tcaaaaatat tccctgttga atggtcggga ttagaagagg tcaataatct cagcttactg
    30301 tcatagagct gatactcacc atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc
    30361 atggtacgca cgggaatact catccccagc acgccatacc agtagttatc actgtctacc
    30421 ggaacactga cggtaacctg cggttcagta ttgctggcgt gttccggttg cgaagtgaac
    30481 cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc cgacaaacca gggttgggtg
    30541 acatagccgt aataacgcgt cggtacattg cgcgtaaaca aggtcggctg cgtcgaaacg
    30601 taaaatccgg cacgcgagac atacatcgcc tgttcaacca ttgacgagga gttgtgcgcc
    30661 agtcgcagca agtaaccaac ttccagcgca gcggtaattt cattgtcgag gctttcattt
    30721 tcgcgagaca ggagattccc ctcgctgact aatgcatccg acacgccatt gactggcagg
    30781 gtgcgtcgtc ggttgagttc gatttgccag gcgtgctcgt cacgatgctg ttcgaactcg
    30841 gttacggcat tacgcagaga ggtgaaatcc agtggcgcta cgagagcttc gcgcatgcca
    30901 ttacgcaaaa agatcagttt gtcgacgtta tactgcaaat gcttatcgag cgcgttggct
    30961 acgttttcca gatgattacg ctggctggag atataggcat cttccagcac caccacttca
    31021 cgccaggtga gcaaggtgga aaaaagcaat accacgataa agcagagatt aacgatatga
    31081 ccaggcccca ggcggcgtgc gagttttttc aaccagctct ggttttccat ttttgtctcg
    31141 tgctgcaccc tgacgactcc caacctgctt ttttatgatt ctggcataac ccggcgataa
    31201 cgtaccgcaa aacagcgata attttataat ggtggcatga taagacgcga ctatcattca
    31261 ttgcaggcat tggcacacca ggccggatgt gatccggcct ggtgttaatg ataccctttc
    31321 aaacttcttc cgcagaacga ctcccctccc ccggaacaag ctcggtttca tgaaaagctt
    31381 cccgcttgac cccgtaaccg tcataccaac gacactcaac cataccgctg gagtatccgg
    31441 tgacaatcat ccgcgggccg ccctctttaa ccgtaacttc ctcactaacc ataaaactca
    31501 tactcgcctc cttttttcga gtgaaacttg ttcaccttag tttaagatgg cgaattttgc
    31561 ataatagcct gtgcgagtat taatgtgcct gaccgcgcat tttcgctact gctttcactc
    31621 ccagcaccac gatgccaccg atgataaaac caagaatcag atttaaaaca gtcggtaata
    31681 tcatcgccac cactgcactt tgctgcccgg cgaaatgttc aatggcgtga tgcagcggcg
    31741 caataccatg taccacaatc ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg
    31801 ataacgcctt catcaaccag ggcgcaatga tcaataaccc tttacctaat gcctgcatca
    31861 gcgcgctgga tttttccgcc agccaatacc ccaggtcatc aatcttaacg ataaccccta
    31921 ccagaccgta gacgcccacg gtcactacca gcgcgatgcc tgaaagcacc agcacctgat
    31981 taagcaacgg cgcttctgcc acaatcccca gcgtgatggc gacgatttcc gcagacaata
    32041 taaaatcggt acgaatcgcc ccttttattt tgtctttttc aaacttcagc ggatcctgcg
    32101 ccgccaactt ctccagacgc tgctgactct gcgccggatc ttctttatgt ttacgcgcct
    32161 ccagcatatg cagcactttc tctacccctt caaagcagag aaacgcgccg ccaatcatca
    32221 acagtggcgt aatcgcccac gggataaacg cactgatgat cagcgccagc ggcaccagaa
    32281 tcactttatt gatcagcgat cctttcgcca cgccccagac cacgggaagt tcccggttgg
    32341 cgcgcacgcc tgaaacttgt tgcgcattga gcgataagtc atcccctaat acaccggcgg
    32401 ttttcttcgc cgccagtttg cccatcacgg aaatatcgtc cagcagtgtg gcgatatcat
    32461 cgagcaacgt cagtaaacta ctcccggcca aaagcattcc ctctcatttt tattgttgaa
    32521 gggtgtaagt atgaagcaaa aacggcagct ctgatacttg cttcacaggt caacattttt
    32581 ttaacaatca agtgcaatta tatctctcgc cagcagaatg attttcacgt ttactatgaa
    32641 ccaccttttt atttccaccg tgagggatta tgcgtttccg ccagttgtta ccgctttttg
    32701 gcgcgttgtt tgcgttgtat atcatttggg gctcaaccta ttttgtcatt cggattggcg
    32761 tggaaagctg gccgccgtta atgatggcgg gcgtgcgatt cctggcagcc ggtattttat
    32821 tgctggcatt tttgctactg cgcggacaca aactcccccc gctacgtccg ctgctcaatg
    32881 ccgcgctgat tggcctgtta ttgctggctg tcggtaatgg catggtaacg gttgccgaac
    32941 atcaaaatgt tccttccggc atcgccgccg tagtggttgc aaccgtgcct ctctttacct
    33001 tgtgcttcag ccgcctattt ggcattaaaa cgcgcaaact ggaatgggtg ggtatcgcca
    33061 ttgggcttgc cggaatcatc atgctcaata gcggtggaaa tttaagcggc aatccgtggg
    33121 gcgcgattct gattttaatc ggctcgatta gctgggcgtt tggctcggtt tatggctcgc
    33181 gcattacatt acctgtaggg atgatggcgg gtgcgattga gatgctggca gcaggcgtgg
    33241 tgttaatgat cgcgtcgatg attgcaggtg aaaaactgac ggcgctccct tccctttcag
    33301 gcttccttgc ggtcggctat ctggcgctgt ttggttcgat tatcgccatt aacgcttata
    33361 tgtatttaat ccgcaacgtc agtccggctc tcgccaccag ctacgcctac gttaacccgg
    33421 tggtcgcggt cttgctgggt acgggactgg gcggagagac gctgtcgaag attgaatggc
    33481 tggcgctcgg cgtaattgtc ttcgcggtgg tactggtcac gttgggaaaa tatctcttcc
    33541 cggcaaaacc cgtagttgcg ccagttattc aggacgcatc aagcgagtaa atgaatcccc
    33601 tgcgtgtcga tctgcgcgct ggcaccttcg ccgcagatcc actcttccag acgctcggta
    33661 agcgcttcat ccttcagctt ctcgcgccca cgtaacgcgc actcccagac aatcaatacg
    33721 cgccagccga gttcctgcaa gcgactgata tcgcggcgat cgcgctcaac atttttacct
    33781 atcttctcca gccagaactc ggttcgtgtc tcaggcactt taaacagata gcagtgatga
    33841 tgatgccaga agcagccgtg ggtaaatatc acgcagcgat attcatcaac gacaaaatcc
    33901 gggcgtccgg ggagatccgc gtcctgaacg cgaaatgcca ggccctgccc ggttaacaga
    33961 ctggcgaggc gcttctcaat cgccgtatca cgcgtagcaa tcgcgcgcat atttttgctg
    34021 cgagtggcct tatcgtgaac gtcggccatg ttgtgcctct tgctgacgca acgccaccgc
    34081 ctgtttgatt tttggctcaa gcagttttgc caccgcggca aagaccggta cgaccaccga
    34141 gttaccgaac tggcgatagg cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc
    34201 cggcgcttca aaccccatta aacgcgcgca ttcacgaggc gttaaccgac gtgggcgatg
    34261 ttgctgattc agcggatcgt caaagtcttt ctcacccgtg gccatatccc aaccgcgatc
    34321 gattaaaatt tccgcgccat ctttgtaata acgcgcagac agcgtacgcg tgacgctttg
    34381 cggattgttc ggataaacca ttccataacc gaagccgtta ccgcgcgcct gatgtttttt
    34441 cgcatatcga tagaggtact tccacagtac cggcgtcagg atatatttcg cctcgaccat
    34501 cgggtccagc agctgcgcca gcgtcactcg ctgtgcaggg aaacatttgc tgatatcacg
    34561 cagagtaaaa tcggctttca gattcagatc gcgacgaaaa cccaccagca cgatgcgttc
    34621 acggtgctgc ggcagaaaat gtttgccatc gatgattttc ggatcgtccg gcccgttatc
    34681 gtctgcgtcg gccacgtcat agcccagttc gtccagcgtc tgcatgatga tgcggaacgt
    34741 tttaccctgg tcgtggcttt tcaggttttt gacgttttcg agcacaaaaa tcgccggtcg
    34801 acgcgcgtcg ataatacgca ccacatcaaa gaacagcgtg ccctgggtat cacaggcaaa
    34861 accgtgcgcc cgcccgagcg agttcttttt cgatacgcca gccagcgaaa atggctgaca
    34921 aggaaaaccc gccagtaata catcgtgttc aggaatgtgt tgacgaatat gttccgccgc
    34981 cgcctcatca ctcacgcctt ctttatggct gagggtgatg tcgcggatat cttcattaaa
    35041 atgatgcgtc gccgggtcgc aataatgatt ggctttataa gtgcgtaccg catgtttgtt
    35101 ccattcgctg gtaaacacgc actgtccgcc aatcgattca aaaccgcgac ggatgccgcc
    35161 aattccggca aacagatcga taaagcgaaa cgcataatgc ggatgatgtg ccggtggttt
    35221 gggtagcaac gtttgcagat gggcgaactc tttctcactt aaacggtgcc atgccgagtc
    35281 attcgccagc gcacgtttta aaattgccgc gctccagtga ttctcaccca caccattaag
    35341 ctgcgccacc agcgttttag catcataaat ttgcagcagt ttctccagca ttgcctgtgc
    35401 ggcattcccg gtgctgtatg aatcggttac tgatatattt tcctgcatag atttaaccgg
    35461 ccatctgaga tggccgacag gttaacataa ttacagccat ttagggccac gcgctcagcc
    35521 cgcgggtgaa aacgcatcta tcaccttgtg atcgacacct tcattctccc ccgccagttc
    35581 ggcactgagc ttcgccataa actcgactaa aaattgcgcg ttgtgctgcg ccagctgctt
    35641 gcctcttgcg gtttgcatgg tttgcggtaa tttcagtaac tttgtctgga aatgatccag
    35701 cgcgtagcgt ttatcatcaa gcgggcgatg ctgtgcgaac ggatcttcgc catcaaacag
    35761 cgccacgccc aatgcccctg aaacggcaaa cacgcgcgcc aggccaatcg cccccagggc
    35821 ttccagccga tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc
    35881 gctgaaactg tgagcggcaa tggcatgaca aacggcctca attttctccg ccggaaattg
    35941 cacgaactct tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg
    36001 ccgttgcgga tgatttttcg ccaggctcac aatatcgtga aaataacagg cggttaaaat
    36061 caccagcata tcgacgtcat catctgccgc cagcttctgt gccgtagccc agacgcggcg
    36121 aaaatgacac acgtcatggg ccgcgtcctg gtgctgatga tggtttttca accagttttc
    36181 gaactctgcc tgccagtgtt gtaagtccat acgcgctccg ttgccaaaga gtcgccacct
    36241 tagcagtttt tattcatagc gtctgctacg tgcgggacga ggtcgggtgt accaggcaat
    36301 gctgcctaac aatgccccga ccagagccat aaataaaaat ccgaccagtg cccctaacca
    36361 ctttcccgcg gtagagccta aatcgctttc aaatcccgac acgggggcat cggggatccg
    36421 gctcatcacc cagatgtaac gcgccattgc ccagacgaaa tagccacacc atgtatagaa
    36481 agcccacaaa gccagtttgc caccagggct gcgagagagt ctctcttcca tctttgtcga
    36541 accgtattcc attttgagat aacccggaaa attcagactt agtactatta tgtgataaaa
    36601 atcacatttt tccacattga atgagccagt caagactcaa tcattttttg aacaaataga
    36661 taatttttgc gcaacggcag tgaatacaag aaaaatcgtt tt
//
LOCUS       DAOTRK010000032        36247 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.032, whole genome shotgun sequence.
ACCESSION   DAOTRK010000032 DAOTRK010000000
VERSION     DAOTRK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.032"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..151
                     /locus_tag="QIE41_001572"
     CDS             <1..151
                     /locus_tag="QIE41_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDS3530474.1"
                     /translation="GFDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIER
                     IKNYL"
     gene            148..639
                     /locus_tag="QIE41_001573"
     CDS             148..639
                     /locus_tag="QIE41_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDS3530475.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQMLYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(680..1630)
                     /gene="corA"
                     /locus_tag="QIE41_001574"
     CDS             complement(680..1630)
                     /gene="corA"
                     /locus_tag="QIE41_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDS3530476.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2000..2763
                     /locus_tag="QIE41_001575"
                     /pseudo
     CDS             2000..2763
                     /locus_tag="QIE41_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
     gene            2846..3748
                     /locus_tag="QIE41_001576"
     CDS             2846..3748
                     /locus_tag="QIE41_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDS3530477.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(3793..5955)
                     /gene="uvrD"
                     /locus_tag="QIE41_001577"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3793..5955)
                     /gene="uvrD"
                     /locus_tag="QIE41_001577"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDS3530478.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(6039..6755)
                     /gene="yigB"
                     /locus_tag="QIE41_001578"
     CDS             complement(6039..6755)
                     /gene="yigB"
                     /locus_tag="QIE41_001578"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDS3530479.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6755..7651)
                     /gene="xerC"
                     /locus_tag="QIE41_001579"
     CDS             complement(6755..7651)
                     /gene="xerC"
                     /locus_tag="QIE41_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDS3530480.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQYDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(7648..8355)
                     /locus_tag="QIE41_001580"
     CDS             complement(7648..8355)
                     /locus_tag="QIE41_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDS3530481.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(8352..9176)
                     /gene="dapF"
                     /locus_tag="QIE41_001581"
     CDS             complement(8352..9176)
                     /gene="dapF"
                     /locus_tag="QIE41_001581"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDS3530482.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTNDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(9213..9416)
                     /locus_tag="QIE41_001582"
     CDS             complement(9213..9416)
                     /locus_tag="QIE41_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDS3530483.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9575..9760
                     /locus_tag="QIE41_001583"
                     /pseudo
     CDS             9575..9760
                     /locus_tag="QIE41_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            9883..10203
                     /gene="cyaY"
                     /locus_tag="QIE41_001584"
     CDS             9883..10203
                     /gene="cyaY"
                     /locus_tag="QIE41_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDS3530484.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(10243..12789)
                     /gene="cyaA"
                     /locus_tag="QIE41_001585"
     CDS             complement(10243..12789)
                     /gene="cyaA"
                     /locus_tag="QIE41_001585"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDS3530485.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            13176..14117
                     /gene="hemC"
                     /locus_tag="QIE41_001586"
     CDS             13176..14117
                     /gene="hemC"
                     /locus_tag="QIE41_001586"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDS3530486.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            14114..14854
                     /gene="hemD"
                     /locus_tag="QIE41_001587"
     CDS             14114..14854
                     /gene="hemD"
                     /locus_tag="QIE41_001587"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDS3530487.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKIADNADNDALLRALQ"
     gene            14876..16099
                     /gene="hemX"
                     /locus_tag="QIE41_001588"
     CDS             14876..16099
                     /gene="hemX"
                     /locus_tag="QIE41_001588"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDS3530488.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQADTPAAAPQGE"
     gene            16102..17298
                     /gene="hemY"
                     /locus_tag="QIE41_001589"
     CDS             16102..17298
                     /gene="hemY"
                     /locus_tag="QIE41_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDS3530489.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADDGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            17977..19632
                     /gene="aslA"
                     /locus_tag="QIE41_001590"
     CDS             17977..19632
                     /gene="aslA"
                     /locus_tag="QIE41_001590"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HDS3530490.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(19734..20969)
                     /locus_tag="QIE41_001591"
     CDS             complement(19734..20969)
                     /locus_tag="QIE41_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDS3530491.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGAEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(21116..21192)
                     /locus_tag="QIE41_001592"
     tRNA            complement(21116..21192)
                     /locus_tag="QIE41_001592"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21156..21158),aa:Pro,seq:tgg)
     gene            complement(21235..21321)
                     /locus_tag="QIE41_001593"
     tRNA            complement(21235..21321)
                     /locus_tag="QIE41_001593"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21285..21287),aa:Leu,seq:cag)
     gene            complement(21342..21417)
                     /locus_tag="QIE41_001594"
     tRNA            complement(21342..21417)
                     /locus_tag="QIE41_001594"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21382..21384),aa:His,seq:gtg)
     gene            complement(21476..21552)
                     /locus_tag="QIE41_001595"
     tRNA            complement(21476..21552)
                     /locus_tag="QIE41_001595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21516..21518),aa:Arg,seq:ccg)
     gene            complement(21655..23040)
                     /locus_tag="QIE41_001596"
     CDS             complement(21655..23040)
                     /locus_tag="QIE41_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDS3530492.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRNGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKIFGL
                     NRHGKAHKLEE"
     gene            complement(23231..23971)
                     /gene="rffM"
                     /locus_tag="QIE41_001597"
     CDS             complement(23231..23971)
                     /gene="rffM"
                     /locus_tag="QIE41_001597"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDS3530493.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQMLFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(23974..25326)
                     /gene="wzyE"
                     /locus_tag="QIE41_001598"
     CDS             complement(23974..25326)
                     /gene="wzyE"
                     /locus_tag="QIE41_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDS3530494.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIHGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(25323..26402)
                     /gene="rffT"
                     /locus_tag="QIE41_001599"
                     /gene_synonym="wecF"
     CDS             complement(25323..26402)
                     /gene="rffT"
                     /locus_tag="QIE41_001599"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDS3530495.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSDHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLALFSEENLQILNEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(26399..27649)
                     /gene="wzxE"
                     /locus_tag="QIE41_001600"
     CDS             complement(26399..27649)
                     /gene="wzxE"
                     /locus_tag="QIE41_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDS3530496.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(27651..28781)
                     /gene="rffA"
                     /locus_tag="QIE41_001601"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(27651..28781)
                     /gene="rffA"
                     /locus_tag="QIE41_001601"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDS3530497.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPVGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(28786..29460)
                     /gene="rffC"
                     /locus_tag="QIE41_001602"
                     /gene_synonym="wecD"
     CDS             complement(28786..29460)
                     /gene="rffC"
                     /locus_tag="QIE41_001602"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDS3530498.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVGDSGAEVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGAIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(29438..30319)
                     /gene="rfbA"
                     /locus_tag="QIE41_001603"
     CDS             complement(29438..30319)
                     /gene="rfbA"
                     /locus_tag="QIE41_001603"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDS3530499.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKLVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(30338..31405)
                     /gene="rffG"
                     /locus_tag="QIE41_001604"
     CDS             complement(30338..31405)
                     /gene="rffG"
                     /locus_tag="QIE41_001604"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDS3530500.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPACVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYHDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(31405..32667)
                     /gene="wecC"
                     /locus_tag="QIE41_001605"
     CDS             complement(31405..32667)
                     /gene="wecC"
                     /locus_tag="QIE41_001605"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDS3530501.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSDRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(32664..33794)
                     /gene="wecB"
                     /locus_tag="QIE41_001606"
     CDS             complement(32664..33794)
                     /gene="wecB"
                     /locus_tag="QIE41_001606"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDS3530502.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALANIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(33850..34896)
                     /gene="wzzE"
                     /locus_tag="QIE41_001607"
     CDS             complement(33850..34896)
                     /gene="wzzE"
                     /locus_tag="QIE41_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDS3530503.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(34908..36011)
                     /gene="wecA"
                     /locus_tag="QIE41_001608"
     CDS             complement(34908..36011)
                     /gene="wecA"
                     /locus_tag="QIE41_001608"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDS3530504.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
BASE COUNT         9129 a         9844 c         9120 g         8154 t
ORIGIN      
        1 tggttttgat gtaactgata taaaaaacaa gacttacagc aaatatagat tttttcgcat
       61 atggttcttt ttacaacggc ataaagactt tgctttcaaa ccttttttca ccaattttaa
      121 cactatcgaa agaatcaaaa attatctatg aaaagtaaac tccagaaaat tatattatgt
      181 ttatttcttc tatgctgcat ctataattta tggacattgc gtcctgtaca gatgttgtat
      241 acatactctg atgctggcaa ttcagtcttt ctggtcgttg atcatctgcc atggactgac
      301 agcgataaaa taaattggta tctgaagcat cagaatgaaa ttaaaaatca acatccccta
      361 ccagaaggta gttggcatac ctggtatgtc attgatatcg gtaatggctt cactgactat
      421 aaaaaatata tcgaagggcc gtatgaagat ctctactgtt tcccgacaat taagagcaat
      481 gataattgca tcgtgaaaaa ttacttaatg gttattaatg aataccctta tcgtaacact
      541 catattggta ttaacgattt tactgagtat caattaacac aagaaaataa aatagagcga
      601 gttttcaatc cgcatgactt taaaaaagac aatttttaga aataagccgg ataaaaaata
      661 gctttatccg gcactcatat tacaaccagt tcttccgctt aaagtacaga tacggtgcca
      721 ggcccgcgag gatcataaag ataatcgcgc ctgggtagcc gaaactccac ttcagttctg
      781 gcataaactc aaagttcatc ccgtagctgg aagcaaccag tgttggcggc aggaacacga
      841 cggataccac cgagaagatt ttgatgatgc ggttctgctc gatgttgata aagcccatcg
      901 ccgcctgcat caggaagttc accttctgga acagagattc gttatgcggc agcagggatt
      961 cgatatctcg caggatttca cgcgcctgct ctagttgccc gcccggtaaa cgcgccttac
     1021 gcaccaggaa gttgagcgca cgctgggtat ccatcagaca caggcgaact ttccagccga
     1081 tatcttccag ttccgccagc gtggagagcg cctcgtcgta ctcatcgccc tgatgccctt
     1141 ccataattac ccggctcaac tgctccaggt cgctgtagat attttcaatt tcatctgcca
     1201 actgttcgat tttggtttcg aacagatcca gcagcagctc gtaggcatta ccgtctacca
     1261 tcgactggct acgggcacgc atacgataca gacggaatgc aggcagttca cgctcacgca
     1321 gagtaaacag acgaccatca cggatggtaa atgccacagt ggagttaccg gcgtgatctt
     1381 ccgcatcttc aaagaagaag aaggagtgaa tatgcaggcc gtcatcgtct tcaaagaaac
     1441 gtgccgatgc ttcgatgtct tccagttccg ggcgggttgc caggctctga ccaagttcag
     1501 attgtacgcg cagtcgctcg tcgtcgtccg gttcgacaag atcaatccat actgcattta
     1561 caaggggttg tgactcttcg acttccagcc gggtcagtcg gttattttcc agttgaaatg
     1621 cgctcagcat gaccgggact cccaatgctt aaaatatcgg acagttcggt gggcacacag
     1681 aaacaaattg ggtttcagac cattaaacag cctgactcag cgcgacggga aaaattgagg
     1741 tcgctgacaa ccgctaaggc tatcagcaaa aagggatagc cttaggagtt gatcctggat
     1801 gacaggatag tgagccagta tctactgggt gtgtccaagg cgaatgtcct cttagagtga
     1861 tcgtgcgcgc atgttacgcc agcaaaattt tgccgtcaac acgcaacaga acaccacaga
     1921 acaatagttg aacttcgcgc tttttggtac tcgtcaaggt cgctaacagg ataaagaaat
     1981 cccacatcca ggacctataa tggcgcatcg gctactcatt ggtaaaggaa tgatcacctt
     2041 gaatctcaaa cggatttttc tcgccctcac cttacttccc ttgtttgctg ttgccgctga
     2101 tgattgcgca ctctcagatc cgacgctgac cgtacaggcg tataccgtta atcctcaaac
     2161 agagcgggtg aaaatgtact ggcaaaaagc caatggcgaa gcgtggggaa cgttacatgc
     2221 tctgctggcg gatatgaata gtcagggtca ggtgcagatg gcgatgaacg gcggcatcta
     2281 tgatgaaagc tatgcgccgc tcggtttgta catcgaaaac ggtcagcaga aggtggcgtt
     2341 aaatctcgct tcaggtgaag ggaatttctt tattcgtcct ggcggcgtgt tttatgtcgc
     2401 gggagataaa gtcggcatcg ttcgtctgga tgcctttaaa accagtaaag agattcagtt
     2461 tgcggtgcag tcagggccaa tgttattaga aaacggcgta attaatccac gcattcaccc
     2521 gaatgttgcc tcgcgtaaaa ttcgtaacgg tgtaggatta ataaacaagg gaacgccgtg
     2581 tttttgttga gccagcaggc gacaaatttt tatgattttg cctgttatgc caaagcgaaa
     2641 ctgaacgttg agcagttgct ttatcttgat ggcacaattt cacatatgta tatgaaaggc
     2701 ggggcaatcc cgtggcaacg ctatcctttt gtcaccatga tttccgtcga gcggaaaggt
     2761 taagcattgt acactcgagg aggccagaca aactgggact ggctacacag ctggaaaatc
     2821 atcagataaa tgaagggaaa caaaaatgaa aaaaacgctc gcaacgttat ttttattaac
     2881 gtgtctggga tcatcatccg cctatgcgga taacgctttg attttacaaa ctgattttag
     2941 cctgaaagat ggtgccgtct cggcgatgaa aggggtcgca ttcggcgttg accataatct
     3001 taaaatcttt gatttaacac acgaaattcc accgtataac atctgggaag gcgcttatcg
     3061 cctgtatcag actgccagtt actggccgca aggttcggtg tttgttagcg tggtcgatcc
     3121 gggcgtaggc actgaccgta aatcggtagt gctgaaaacc aaaaacggtc aatacttcgt
     3181 ttcaccagat aacggtacgc tcactttggt ggcacaaact ctgggtattg atagcgtccg
     3241 ggaaatcgac gaaaaagcta accgcctgaa aggttctgaa aagtcatata ccttccatgg
     3301 tcgcgatgta tatgcctaca ctggtgcgcg tctggcttcc ggagcgatta cgttcgaaca
     3361 ggtggggcca gagcttcctg caaaagttgt cgaactttct tatcagaaag caaaagcaac
     3421 aaaaggtgaa gtgaagggca atatcccgat tctcgatatt cagtacggca acgtctggag
     3481 caatatcagc gacgagttgc tgaatcaggc aggaatcaaa ctcaacgaca cgttgtgtgt
     3541 gaccatttcc gaaggttccc agcaaaaata cgcagggaaa atgccgtatg tcgcaagctt
     3601 cggcgatgtg ccggaaggcc agccgatggt gtatctgaac agcttattga atgtttccgt
     3661 ggcgctgaat atggataatt ttgcgcagaa acatcaggtt gcgtctggtg ctgactggaa
     3721 tattgatgtg aagaagtgcg ctaagtaagg tgctgatgcg gaatgcggtg tggcgcaccg
     3781 catcccgcaa cgtcacaccg tctccaaccg ggcataagcc gccaccagcc atttaatacc
     3841 ctggccctgg aacgccacct gcaaacggct atgctcaccg ctgccttcca tattgacaat
     3901 ggtgccttca ccaaacttag cgtggcgtac gcgctggccg agcttgtagc cgctgtcgtt
     3961 ctcgaccatc ggcgtaccca tccgttgatg gctgaccggg cggcttaccg tggcgcgcag
     4021 gcgcacctct tccacacact cttccggcag ctcgccgata aagcgcgacg ggcgatggta
     4081 aacttctttg ccatacagac ggcgggtttc cgcgtaggtc agcgtcagtt tctgcatcgc
     4141 gcgggttacg ccaacgtagg ccagacggcg ttcttcttcc agacgcccac cttcatccag
     4201 cgacatctgg cttgggaaca tgccctcttc cataccgacg ataaacacct gcgggaactc
     4261 cagacctttc gccgagtgta gcgtcatcaa ctgcaccgca tcctgccagg tatccgcctg
     4321 cccctcgccc gcttccagtg ccgcatggga gaggaacgcc tgcagcggca ttaaatcttc
     4381 gtcttcttcg ttgtagctga actggcgcgt cgccgtcacc agttcctcta agttttcgat
     4441 acgcgtctgg cctttttcgc ctttctcctg ctcgtacatt gtgcgcaggc cggagtcttt
     4501 aattacccgg tcagtctgta catgcagcgg catatcggca gtttcctgcg ctaaggcgtc
     4561 gatcagttcc ataaaccgct gtaaggcact ggcagcacgt ccggcgaggg ctttttcctg
     4621 caacagttca cgacatgcct gccagagtgt taactggcga tcgcgcgatg tctgacgtac
     4681 cacgtccagc gtccggtcac caataccccg cgttggtgta ttcaccacgc gctcaaaggc
     4741 cgcgtcgtca ttacggttgg caatcaggcg cagatacgag agcgcatctt tgatttcctg
     4801 gcgttcgaag aatcgcatcc cgccgtaaat acggtacggc atacttgcct gtaacagtgc
     4861 ttcttccagc acacgcgact gggcgttgct gcggtagaga atggcgcact cggcaagcgc
     4921 cccgccgttg tcctgccagg ttttgatacg gttaaccaca aaacgcgctt catcgagttc
     4981 gttaaaagcg caatagaggg aaataggctc accatccgcg ccatcggtcc acagtttttt
     5041 acccaaacgc ccgttgttgt tttcaatcag ggcgttcgcg gcgctcagaa tattgctggt
     5101 agagcggtag ttctgctcca gacggatagt ttcagcaccg gggaaatcat tgaggaaacg
     5161 ctgaatattc tcgacctgcg ccccacgcca gccgtagatt gactggtcgt catcgccaac
     5221 gatcatcact ttgccggtgt cgcccgccag caggcggatc cacgcgtact gaatgttgtt
     5281 ggtatcctgg aattcgtcta ccaggatgtt agtaaaacgt tcgcggtagt gttgcagaat
     5341 atgcggcttg ttaagccaca gctcgtgcgc gcgtagcagc agttccgcga agtccaccag
     5401 gcccgcgcgg tcacacgctt cctgatacgc ctgatacacc ttctgccagg tctgctccac
     5461 cggattaccg tagctttgaa tatgatgtgg acgcaggcct tcatctttct ggctgttgat
     5521 gtaccacatt gcctggcgcg gcggccactg cttctcgtcg aggttcatcg ccttgattag
     5581 gcgtttgagc aggcgtagct ggtcctcgct gtcgaggatc tgaaaatcct gcggcagatt
     5641 ggcgtccata tggtgcgcac gcagcagacg gtgcgccagc ccgtggaagg tgccgaccca
     5701 catgccgccc tggctggtgc ccatcagctg cccgatacga tgacgcatct ccgccgccgc
     5761 tttgttggta aacgttaccg ccataatcga atatggcgag cagttttcca cgctcatcag
     5821 ccaggcgata cggtgcacca gtacgcgcgt cttaccactg cccgcgcccg ccagcaccag
     5881 aaggttgctg cgtggcgcgg ccaccgcttc gcgctgtttg tcattaaggc tgtcgagcag
     5941 gtaagaaacg tccattggca ccgccgcgta aaaataggtt gggcggagaa gcgcaacgcc
     6001 ctccgccaaa aagctgggta tatatacaga tttgctgatt atatcagcga ggtcagagat
     6061 gccaaccggg aaatttccag atgcgggagt aaacggctgt cccaggtttg catcagatcg
     6121 ccattttccg gtctgatcca acaagcctgc attccgctgc gaattgcccc acccacgtca
     6181 gtggtgagat cgtccccaac atgtaagatc tcgccgatcg gcacgttgag tttttccgca
     6241 gccagaaagt acatatcgct gaacggtttt gagcgcccgt gcgggccagc gcgcagcaca
     6301 aactcaaaat aatcccctaa accaaacagc tccggctggg cgttaccgtt ggtgatcgcc
     6361 accagcggcc atttcttcgc cagctgtttt aaggtgtcgt gggtttgctg cggaacatcg
     6421 attcggctgc gccatttggc aaagttgatc attgctgcgt gtgcgcctgc actggcttct
     6481 tcggcactca gcccggcgtc gagcatggct tgttcaatcg aacgaaaacg ccagcgcgtc
     6541 acgtcgtgat aaatctcggg ttccgcttcc cgtaccgcct ggcgcaggcg ttgcagatct
     6601 tcattctgga agctgcgcag cgccggatga taattttgca caaaggtaag cgcctctcgc
     6661 tcggtgcgca aaatcaccgg acggttatcg taaagggtat catccaggtc aaaggtgagc
     6721 gccgagatgc gccccaaagg ccggtaaaaa cgcattattt cccccgtttg gcgcgtggat
     6781 gcgccgcatc gtacaccgag gcaaggtgtt gaaaatcaag atgagtatag atttgcgtgg
     6841 tggagaggtt ggcatgaccc agcagctcct gcacaccacg aagatcgccg ctcgactcca
     6901 gcatatgcgt agcgaacgag tgacgtaatt tatgcgggtg aacgtgatta ttcagccctt
     6961 gttttatgcc ccattcggca aagcgtttct gcacattacg cgcggagata cgcttgccca
     7021 gtttcgacag aaaaagcgca tcgtcttcgc ttccaaacag gtcgcgcaaa tcaagccagt
     7081 gctcaatcca cgccacagcg ttgcgaccaa tcggcaggcg gcgctctttg ctgccttttc
     7141 ccatcaccca cacttcacca gactccaggt cgaggtgttt gatatcgagc cccaccagct
     7201 cggaaagacg cagacccgcg ccgtacatca cttccagcat tgcccgatcg cgcacagcga
     7261 ggggatcgtt gatatcaata tccagcagcc gattcatatc gtcgacgtcg atgtttttcg
     7321 gcagatgacg cggcgctttc ggtgccgaaa cacctttcgc cgggttagct ttgagctcgt
     7381 tctggctgac cagccagtca aaaaagctac gtagcgcaga aagccgtaac gccaaacttg
     7441 cagctcccag ccctttacgg cgactgcgta cagcgaaatt gcgcaccatc gtcacatcat
     7501 attgctgcca gctttgcagg ccgttttcgc tggcaaaatt gatgatcgcc tcaagctgac
     7561 gctggtagtt aagcagggtt atcgggctta gctggcgctc cacgctcaga taacgtaggt
     7621 agcgttctac atcggtgtgt aaatcggtca tacgcgttca atccaacgct ccagaagctc
     7681 cggcaacatc agcgcaattt catgaagtaa ctgcgttccc tgcccttgtt gatagtgact
     7741 ggcatcgcga ctggtaaaca gcacaacacc caaatcagca tcgcttccca gcatcgacat
     7801 cgccaccgat cccaccgctt tcgcttccgg tagcaccacc agaagctccg gtccgttaag
     7861 cggccccaga tagtgctgtt cctgccccag acgctgaata cgcagcggtt cgaaagactg
     7921 acggcttaat gccagatgag tgtggttcga cggcgcacct aagcgccagc gatctggaaa
     7981 caggcgcaga ctcgcacctg ccaggccgag atcgcgcgcc cagcggtgaa agcgcatcag
     8041 catatcgtcg agactgctgg cggcggtgag actacgctgc aggtagagta agcgataaaa
     8101 caggccttcg ttggcgatag cctgttccat caacagcgcc atgttctctt ccagaacatg
     8161 aatatgatta cgtgcgcggg ccatgtgcca ttcgaccaac gaaacggtgc cgcgtaccgg
     8221 atgcggcaca cgtatcgctt caactgcgcg cgcattgcgg ataaaaaact caggattttt
     8281 aatcagataa tcgacaaccg cccggtcatc aagctccgtg agtgtttcct gcagttcttc
     8341 ccctggttgc ttcatagatg aataaatccg tcgtagacat gtaccgccgg gccagtcata
     8401 tataacgggt gacccggacc tttccaggcg atatcaagac gaccgccggg gagttccacg
     8461 cgtacttctt cggccagcaa accttgctgg atccctaccg ccaccgccgc acacgcgccg
     8521 ctgccgcagg cctgggtttc tcctgcccca cgctcataaa cgcgtaaacg aatatgctcg
     8581 cgcttaacca cttgcataaa accgatattg gcgcgctccg gaaaacgctc gtggctttcc
     8641 agaacaggac caagcgtttc taccgccgcg gtatcgacat catcgacctg aatcacgcag
     8701 tgcggatttc ccatcgacac cacgccgcat aagattgtct gctcggcggc gcgcataata
     8761 taggtctttt ccgctttgtt agcgcgaaat ggcacggcag aaggttcgaa gttgggttcg
     8821 cccatattga cgcggaccag atcatcattg gtgacggtca gaaccatccg tccgttggcg
     8881 gtgctgacgc ggatatcgcg cttattggtc agccctttca gacgcacaaa acgggcaaag
     8941 cagcgcgcgc cgttgccgca ctgcgccact tcactgccat cagcattgaa aatgcgatag
     9001 tgaaaatcca gttcaggatc atacggtggc tcaaccacca gcagttggtc aaaccctacc
     9061 cccaggtgcc gatccgccag gcgacgaatc agctccggtg aaaaaaagac attctgcgtt
     9121 accgcgtcga cgaccataaa atcgttgcca aggccatgca ttttcgagaa ctgcatcatt
     9181 tactccaatc acgcgggtac agaaactgac ttttagtaat tcacctggga tggaccatcg
     9241 ccagtggcgc ggtcgttttt atccggcact gtggattgcg tttgcgtctc taccggtttg
     9301 gtcggcggcg gtgcgttttt atctgcaggc gggaaataga gcggaccttt cagaccgcag
     9361 cccgtcaggc tgaagagagt aagtaataca gtgagtgcct taaacacgtt tttcattatg
     9421 gattgcctgt aaagttcatc gctttctgct ttctatcatc gcaggagaag gcgtaaaagc
     9481 aagcgtttag cgactcttta tgaagtctga aaagtgagga aatgctgcga gtataacgct
     9541 tactcgtggc gatttatgtt ttgctggtag tttcgtgatc ggcacctcgc gccaggatgg
     9601 ctttatgcac tatcgcaatg tggtttgttt gttggcttgt tcgttgtttt tatcgtcggc
     9661 atggggatgt cggcttgatg agccggaaca taatatttac cagaaacagg gaaaaggcgt
     9721 ggtgtatttg cgcccttatg aaaagaccaa tctttcgcta tatagagaca ttattattcc
     9781 gttgacggat gattcatgcc agaacgcaga agacgagcaa actttgaagc cctgacccca
     9841 tgccgctata ctgccgccat cacttaaaaa caggatacaa caatgaacga cagtgaattt
     9901 catcgcctgg ctgatcaact gtggctgacc attgaagagc gtctggacga ctgggatggc
     9961 gacagcgata tcgactgtga aatcaacggc ggtgtactga ccattacctt tgaaaacggc
    10021 agcaaaatca ttatcaaccg ccaggagccg ctgcaccagg tatggctggc aaccaaacaa
    10081 ggcggctacc attttgatct gaaaggcgat gagtggattt gcgatcgtag cggcgaaacc
    10141 ttctgggatt tgctggaaca ggcggcgacg cagcaggcgg gtgaaacagt cagtttccgc
    10201 taagattgca tgccggataa gcctcgcttt ccggcacgtt catcacgaaa aatactgctg
    10261 taatagcggc gtatcgtgat cctgattggc aggcggcatg ttgccaatcg atttggtgcg
    10321 gaacggaatc acctgttcac gaccatcaac cttcacaatc tgatagaact gcggcaggtt
    10381 gaagttgatg aagcttgagc cgtaggtaaa acggtcgtgc gatgacgagt agaagcgact
    10441 gacgtcacgc accagctcct ctttgctgcc ttcgcagtgg tgatacacct caacccggtt
    10501 gctttcgtcg agaatgtaga tattaaagcc attctcgtct tgcgtttctt cgaagaagaa
    10561 ctggatgatc ccttcgctgg caaagccgtc caccaccgcc ggtaatttga cgtgattggt
    10621 ttcaacctgc actgacaggc cgtgcagttt gttatgcgaa atagcgccat aaaactcgat
    10681 ggcattttct agtttctgta ccgatacatt caggcgctcg aagaataagc cccaggtttg
    10741 accagaaacg cgcagcgcct tgaagcgccc ggtttcctgg cgggtgctgg aaagacgcag
    10801 ttcaatgcac tcagaaacca gttgctgcac gcgagtacgg attaatccgc gcagatgctg
    10861 gctgtagcag aagacttcca cgctatctgg cggtgcggcg tcctgatgca ttttaccgag
    10921 aatggttttc agggcttcga tcatcgactg ctcgccgttg aagtgcaacg tacgcacttc
    10981 gttccacgag ttgcggtaca gcaggtccac gctacctacc aggcaatttt gattctcgcc
    11041 aaagctgaag acgtccagct tacggaaatc aaaatgcacc acctgattgc ggaacgcagc
    11101 tgtcgggtca tattccaggt taacgataat cgccagatgg cgaatttcac acgggctgta
    11161 gagcgctttt ggcgtcggtg caggcaggcg cagcgggaaa tggtgtgaca catcggcgac
    11221 catctcctgc aacttaggca aatcgacaat gccgttgcct ttaatataca agcgggtacg
    11281 cgaggtcagc aggccgttaa accatgccca cgccaccagt ttattcaggt aacggttata
    11341 ttccagcggc tgatggctga tgatcgactc aatatttggc gcgcggttat acagatacca
    11401 acctgaacgg ttagcccggc ccggcggcac ataaataaag gtcagattcg gttccgagag
    11461 atcgggtgaa atctgcgggt ttaccagcgt cactttaccc ggcaatgctt caaacgcggc
    11521 atacagctta cgcgtcagca cgccgatatc ctgcggactg gcggagacgc taaggttatt
    11581 gcggcgcgca aagcgaatca gattacggta gctctgcatc atcgcgtcga gcaactcgtt
    11641 gtgcgcctca cgtacctgat caatcttcca gttagcgcgg ttatcgagca ttgccagacg
    11701 agcatcgtcc cattcccact ctttcactaa ctgggttagc acttcacgac gccagcctac
    11761 gcaggcgcgt tcacggctga gtttttcgca cacttttaaa tagaagcagc ggcgtactaa
    11821 atccagacga gtgaaatctt caatcgccgt caggtattca gtaacacgct ccagcatcat
    11881 gcagtaagga tcgaggccaa acgacacaat ctcgccgtcg tgcaaacgct gtttgatatc
    11941 tttcgccagc agacgtgggt tcgggtattc ccaggaatag gcttccagca gcagtgtttt
    12001 cagcaccgct ttgtatgggg aatcaatact cttgtagagc tgccaaaggc tggcaccaaa
    12061 gtactcttca gcagaaagcg agcttaagcc accgagatcc agccattcat ttggcgtcag
    12121 cacgccctgc gcgtaaagcg tcatcacata gtcgtcgtaa tgctcttctt cgtcgcacgg
    12181 caccatattc cacagaatac gcttaccagc gagacgcacg gcggtacggt aaaattcgtc
    12241 aagcagcagt atatgctggg tggagccaca atcttcgccc cccaggctgc cgctttcatt
    12301 atgacggaag cgattttcat caatcaggaa gaagctaact tccacgccca gcgaggcggc
    12361 ccagctttcc agcaggctgc atttacgttg tagtaactgg cgctcttcgc tatcaagcca
    12421 ggattgatga cagacccaga tatccaggtc agaggaacaa ctttgcccta cggacgaggt
    12481 gctgcccatg gtgtatacac cagtaattgg aagctcacct ttcggcggat cctgtactga
    12541 cattccacga tacagttcaa gctcgttcag gtagtggcgt tgagtttcat caggcgtgta
    12601 aaggcaaatg cctttgggaa cgttaccatc aaggtaaccc ggcattagcg gatggtgata
    12661 gtgcaacaat gtcggcagta gactgtagac ctgttggaat gcaggcccca tagcagcaag
    12721 cgcgcgatcc acacgcaatt gatttatggc atccagtctc tgtttcagag tctcaatata
    12781 gaggtacaag acgtatcgcc tgatttgcta cccgtcatga ctgtgattcc gccaacatca
    12841 acggtaacac gcggcattcg ggatatttcg tatgtcaaag gtaaccgtta ccacttttcg
    12901 cgcctggttt ttttagtttc acgacgaaaa aatggtctaa aacgtgatca atttaacacc
    12961 ttgctgattg accgtaaaga aagatgcgct acatacaagt gtagcaccgt ttattctctg
    13021 taaattcctt attacgacgg cgtgaaacgc ctgtcaggat ccactgccag acctcatttt
    13081 acggtttgcg caggcgtcta cgtttcacca caacactgac atcgctctgg caaggatgtt
    13141 aggatgggcc acggatgata atgacggtaa caagcatgtt agacaatgtt ttaagaattg
    13201 ccacacgcca aagcccactt gcactctggc aggcacacta tgtcaaagac aagttgatgg
    13261 cgagccatcc gggcctggtc gttgaactgg taccgatggt gacgcgcggc gatgtgattc
    13321 ttgatacgcc gctggcgaaa gtaggcggaa aaggcttatt tgtaaaagag ctggaagtcg
    13381 cgctcctcga aaatcgcgcc gatatcgccg tacattcaat gaaagatgtg ccggttgaat
    13441 tcccgcaagg tctgggactg gtcactattt gtgagcgtga agatcctcgc gatgcctttg
    13501 tgtccaataa ctatgacagt ctggatgcgt taccggcagg cagtatcgtc gggacgtcca
    13561 gtttacgtcg ccagtgccaa ctggctgaac gccgcccgga tctgattatc cgctccctgc
    13621 gcggcaacgt cggcactcgc ctgagcaaac tggataacgg cgaatacgat gccatcattc
    13681 ttgccgtagc cggactaaaa cgtttaggtc tggagtcacg cattcgcgcc gcgttgccac
    13741 ccgagatttc tcttccggcg gtaggacaag gtgcggtcgg tattgaatgc cgccttgatg
    13801 atacacgcac tcgcgagctg cttgccgcgc tgaatcacca cgaaactgca ctgcgcgtta
    13861 ccgcagaacg cgccatgaat acccgtctcg aaggcggatg tcaggtacca attggtagct
    13921 acgccgagct tattgatggc gaaatctggc tgcgtgcgct ggtcggcgcg ccggacggtt
    13981 cgcagattat tcgcggtgaa cgccgcggtg cgccgcaaga tgccgaacaa atggggattt
    14041 cgctggcaga agagctactg aataacggcg cgcgcgagat ccttgctgaa gtctataacg
    14101 gagacgcccc ggcatgagta tcctggtcac ccgcccgtct cccgctggag aagagttagt
    14161 gagccgtctg cgcacactgg ggcaggtggc ctggcatttt ccgctgattg agttttctcc
    14221 gggtcgacaa ttaccgcaac ttgctgatca acttgcagcg ctgggggaga gcgatctgtt
    14281 gtttgccctc tcgcaacacg cggttgcttt tgcccaatca cagctgcatc agcaagatcg
    14341 taaatggccc cgactacctg attatttcgc cattggacgc accaccgcac tggcgctaca
    14401 tactgtaagc ggacagaaga ttctctaccc gcaggatcgg gaaatcagcg aagtcttgct
    14461 acaattacct gaattacaaa atattgcggg caaacgtgcg ctgatattac gtggcaatgg
    14521 cggtcgtgag ctaattgggg ataccctgac ggcgcgcggt gctgaggtca ctttttgtga
    14581 atgttatcaa cgatgcgcaa tccattacga tggtgcagaa gaagcgatgc gctggcaatc
    14641 ccgcgaggtg acgacggtcg ttgttaccag cggtgaaatg ttgcagcaac tctggtcgct
    14701 gatcccacaa tggtatcgtg agcactggtt actacactgt cgactattgg tcgtcagtga
    14761 gcgtttggcg aaactcgccc gggaactggg ctggcaagac attaagatcg ccgataacgc
    14821 tgacaacgat gcgcttttac gggcattaca ataactctca taacaggaag ccataatgac
    14881 ggaacaagaa aaaacctccg ccgtggttga agagaccagg gaggccgtgg acaccacgtc
    14941 acaacctgtc gcaacagaaa aaaagagtaa gaacaatacc gcattgattc tcagcgcggt
    15001 ggctatcgct attgctctgg cggcgggcgt cggtttgtat ggctggggta aacaacaggc
    15061 cgtcaatcag accgccacca gcgatgccct ggctaaccaa ctgacggcat tgcaaaaagc
    15121 ccaggagagc caaaaagccg agctggaagg cattatcaag caacaggcta cgcagcttga
    15181 acaggcgaat cgtcagcaag aaacgctggc aaaacagttg gatgaagtcc aacaaaaggt
    15241 cgccaccatt tccggcagcg atgctaaaac ctggctgctg gctcaggccg attttctggt
    15301 gaaactcgca ggacggaagc tgtggagcga tcaagacgtc acgaccgctg cagcgttgct
    15361 gaaaagtgca gatgccagcc tggcggatat gaatgacccg agtctgatta ccgttcgtcg
    15421 ggcaattacc gatgatatcg ccagcctttc tgcggtatcg caggtggatt atgacggcat
    15481 catccttaag cttaatcagc tttcaaatca ggtagataac ctgcgtctgg ccgataatga
    15541 cagcgatggt tcgccgatgg attccgacgg tgaagagctt tccagttcca tcagcgaatg
    15601 gcgtatcaat ctacaaaaaa gctggcagaa ttttatggac aacttcatta cgattcgccg
    15661 tcgtgatgac accgccgtac cgctgttagc gccaaatcag gatatctatc tgcgcgaaaa
    15721 tattcgctct cgcctgctgg tcgcagcaca agctgtaccg cgtcaccagc aagagactta
    15781 tcgccaggcg ctggagaacg tctccacctg ggtacgtgct tactacgata ctgatgatgc
    15841 caccaccaaa gcgttcctcg acgaggtgga ccagttaagc cagcaaaata tctcgatgga
    15901 tcttccggaa accctgcaaa gccaggcgat gctggaaaaa ctgatgcaga ctcgcgtgcg
    15961 taacctgctg gcacaaccgg cagcggggac aacggaagct aaacctgcac ctgcacctgc
    16021 acctgcacct gcacctgcac ctgcacctgc acctgcaccg caagctgata ctccggcagc
    16081 cgcgccgcaa ggagaataac catgctaaaa gtgttattgc tctttgtttt gctgattgcg
    16141 gggatagtgg ttggcccgat gattgccggt catcagggtt acgtgctgat ccagaccgac
    16201 aactacaata tcgaaaccag cgtcactggc ctggcgatta ttttgattct ggcgatggta
    16261 gtgctgtttg ccattgagtg gctactgcgg cggatcttcc gcacaggcgc acacacccgt
    16321 gggtggtttg tcggacgtaa gcgtcgccgt gcccgtaagc agaccgagca ggcgctgctg
    16381 aaactagcgg aaggcgatta tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac
    16441 gctgaacaac cggtggtgaa ctatctactg gctgccgaag ccgcgcaaca acgtggtgat
    16501 gaagcacgcg ccaaccaaca tctggaacgt gcagcggagc tggccggcaa cgacaccatt
    16561 ccggtagaaa tcacccgtgt acgtcttcaa ctggcccgta atgaaaacca tgctgcacgc
    16621 cacggcgtgg ataagctgct ggaagttacg ccacgccatc cggaagtatt acgtctggcg
    16681 gaacaggcgt atatccgcac aggtgcatgg agttcgctgc tggatatcat cccatcaatg
    16741 gcgaaagccc acgttggcga tgaagaacat cgtgcaatgc tggaacaaca ggcatggatt
    16801 ggcctgatgg atcaggcgcg cgccgatgac ggtagcgaag gtttgcgaaa ctggtggaaa
    16861 aaccaaagcc ggaaaacgcg tcatcaggtg gcgttacagg tggcaatggc ggaacatctt
    16921 attgaatgtg acgatcatga tactgctcag caaattatca tcgacggcct gaaacgccag
    16981 tatgatgatc gtctgctgct gccgatcccg cgtctgaaaa ccaataaccc ggaacagctg
    17041 gaaaaagtgc tacgccagca aatcaaaaac gtcggtgatc gcccgctgtt gtggagcaca
    17101 ttgggtcagt cactgatgaa gcacggtgaa tggcaggaag catcgctcgc cttccgcgcg
    17161 gcgctgaaac aacgtccgga cgcctacgat tacgcatggc tcgccgacgc gctggacaga
    17221 ctgcacaaac cagaagaagc tgcagcgatg cgccgcgacg gtttgatgtt aacgttgcag
    17281 aacaacccgc cacagtagtt ctttctcacc cggaggcaag cacctccggg acctttctga
    17341 tacataaaaa aacgcctgct cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa
    17401 caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg atgaggcata agcgacatct
    17461 gtcagtggac gataagcacc gtaaacggct ctgcgtcatt ccggagttta tgaggcacta
    17521 aggcgaacat aagagatgga atgagcatct actcgtttat tatgccacag agaaccggga
    17581 aataacatcc cttaacactt tttatgagat aattctgtaa tcctctttgc ttcctgagta
    17641 ataacttcct gagtgaatat ttctcctgcg cacgattcta cacatatata taatgaataa
    17701 taaaagtcat tcaattaata acatatatat taattgccgt taaaactaaa aacagcatca
    17761 ataatcaacg cgatataaca tcaccgcctt acatatcacc tgcgccagag gtaggattga
    17821 aaacgctctc ctgattttcc aattcattat ctggataaat aaataattta ttttcatcac
    17881 tattatttat gcaatcatcc tgtcagggag agggatctca attatcaatg cttaattacg
    17941 tcaacatttt gacgacatga aaccggagca ttttgcatgg aattttcgtt ttcacccaaa
    18001 cttcttgttg tggctgtcgc cgccgctctt ccactcatgg ccaacgcagc agataccccg
    18061 tcaaccgcca ccgcacgcaa aggctttgcc ggatacgatc acccaaacca gtatctggtt
    18121 aaaccggcga ccactattgc cgacaacatg atgccggtga tgcagcatcc tgcacaagac
    18181 aaagaaaccc agcagaagct ggcagaactt gagaaaaaaa ccggtaagaa accgaacgtg
    18241 gttgttttct tgctggatga tgtgggctgg atggatgttg gctttaacgg cggcggcgtg
    18301 gcggtgggta atcctacacc agatatcgac gccgttgcca gccaggggct gattttaact
    18361 tcggcgtatt ctcaaccgag ttcttcccca acccgcgcca cgattcttac cggacaatac
    18421 tccatccacc acggcattct gatgccgcca atgtacgggc aaccgggcgg tctgcaaggg
    18481 ttaaccactc tgccgcagtt gctgcacgat cagggctacg tcactcaggc catcgggaaa
    18541 tggcatatgg gggaaaacaa agagtcgcag ccgcagaacg ttggctttga tgatttccgc
    18601 ggctttaact cggtatccga tatgtatacc gaatggcgtg atgttcacgt caacccggaa
    18661 gtcgccctga gcccggatcg ttctgaatac atccaaaaac tgccgttcag caaagatgac
    18721 gttcatgccg tgcgcggcgg tgaacaggaa gccattgccg acattacgcc gaaatatatg
    18781 gaagacctgg atcaacgctg gatggaatat ggcgttaagt tcctcgacaa gatggcgaag
    18841 agcgataagc ctttcttcct ctactacggc actcgcggct gtcacttcga taactaccca
    18901 aatgcgaaat atgcgggtag ctctccggcg cgcacctctt acggcgactg catggtggag
    18961 atgaacgata tcttcgccaa tctgtataaa gcactggaga aaaacggtca gcttgataac
    19021 acgctgattg tgttcacctc tgataacggt ccggaagccg aagttccgcc acacggtcgc
    19081 acgccgttcc gcggtgcgaa aggttctaca tgggaaggcg gcgttcgtgt gccaaccttc
    19141 gtttactgga aaggcatgat ccaaccgcgt aaatctgacg gcattgtcga tctggcggat
    19201 ctcttcccta cggcgctgga tctggcgggg catcctgggg cgaaagtagc gaatttagta
    19261 ccgaaaacca cctttatcga tggtgtggac cagacatctt tcttcctggg aacaaatggt
    19321 cagtctaacc gtaaggccga gcactacttc ctcaacggta aactttctgc tgtgcgtatg
    19381 gatgagttca agtatcacgt cctgatccag caaccgtacg cgtataccca gagcgggtat
    19441 cagggtggat tcaccggcac agtaatgcaa acggcgggat cgtcggtgtt taacctgtac
    19501 accgatccgc aggaaagcga ctccatcggc gtgcgccata tcccaatggg tgtaccgcta
    19561 cagaccgaaa tgcacgcgta tatggagatc ctgaaaaaat atccaccacg cgcgcagatt
    19621 aaatctgatt aagccggcgc tatcgctaca tcgggccaca ctgaccttgc cggatgcgac
    19681 gtgaacgcct tatccggcaa ttaaccgcag gcggccacaa aatggccgct tttttactta
    19741 ttcaccacca tcaaatgcgc ctgcataatg tcgctggccg gacgaccgtg cgccagcaaa
    19801 tcagccattg ctttaagata tggcggtaga tggcggaaat aacgctgata cccggcacac
    19861 aaataattca gcccgggttt gccgctggca tcgagcataa agcggtgttt cggacagcct
    19921 ccccagcacg cttttaacac gttgcaactg cgacactgcg ccggtaactg cttaaattta
    19981 tcttcaccaa acacctgctg ttgcggggaa tctaccattt ctgcaattgt ttgctggtgc
    20041 atattcccca gtcgatattg cggataaaca tagtgatcgc aggcgtaaac gtcgccgttg
    20101 tgttcaacaa tcaccgagcg cccacaggtt ggctgatgat ggcaaaccgc acctggcgca
    20161 ccaacaaaat tggcaaacgc ccattcgata ttcatcacga aaatcttgcc gacgtcgcgt
    20221 ttgatccagt ggtcgaatat cgccaccaaa aattcaccga actcatcggg gcgtaccgac
    20281 cattccgtca gttcgccctg aatatcgccg ggagcatgta gcttaagtcc agcatgagca
    20341 gccgtttcat cagccaggcg ctcgaccacc ggaataaact ggatgaactc agcgcctgca
    20401 tcacacagaa aatcatagac ttgcaaaggt tgtaacgcac tggtgcgatt aacgcagacc
    20461 agcacgttat agtcgacatg atgtttttgc aggagcgtca gggcacgcat caccagctta
    20521 tgcgttggtc tgccgccttt ggtcacgcga tattgattgt ggatctcagc cggaccatcc
    20581 agcgataacc ctacaagaaa atgattttct gccagaaatg cacaccattc gtcatcaagc
    20641 agaacgccgt tagtctggaa gctgttactt atcttcctgc cagcaccata tttcgcctgt
    20701 agcgccacgg cacggcggta aaaatccagc ccgagtaacg ttggttcgcc gccctgccag
    20761 gtaaaagcca cttcgttttg ggtttcgctg gcagcgatat agtgacggac atacgcttcc
    20821 agcgtgtcat cgtccatatg cgtgactggc ttttcgcggt aaagggattg tttttcgaga
    20881 taaaaacagt agtcacagtt cagattacaa tcggaaccgc tcggtttcgc catcacatga
    20941 aaagcacgcg ttggaacctg ttgcagcatg gtcgctcctt gcctgaagtc atccttcaat
    21001 aagttaaggg cgaaatcata ccgtggcgca atgagttatt acgggcgttt attgcgaagg
    21061 gaaagagacg aaatgcacag aaaacaaaaa accccgccga agcggggttc aaaattggtc
    21121 ggcgagagag gattcgaacc tccgacccac tggtcccaaa ccagttgcgc taccaagctg
    21181 cgctactcgc cgaaatactg ctttttgaat ttttagttca attctttaaa gtcgtggtgc
    21241 gagggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag ctagcgcgtc
    21301 taccaattcc gccaccttcg cattgtcaca acttctaata atggggtggc taatgggatt
    21361 cgaacccacg acaactggaa tcacaatcca gggctctacc aactgagcta tagccaccac
    21421 tacaaatctt gttacgcggt attactacca ccgcagctca agcgccggga ctaaatggcg
    21481 cgcccgacag gattcgaacc tgagacctct gcctccggag ggcagcgctc tatccagctg
    21541 agctacgggc gcttagcgcc gttgcggggg tggataatac ggacttccca cccctctgtc
    21601 tagtcccttt ttaaataaat tgcgcgtttg gttacgattt gtgcgttttg ctgcttattc
    21661 ctccagttta tgcgctttac cgtggcgatt aagcccaaaa attttataaa tcgccgtcac
    21721 cgccagcata aagatgatgc cgacaaacag cgacatacgc gtatcttcat taaagtacat
    21781 gccgatcaaa acgcaaatca ggaatgccat tgttacgtag ttggcccacg ggaacaggat
    21841 tgagcggaac ggatggctgg caatcgccgc tttatgcgca cgacgaaaac gcagctggct
    21901 tatcaatatc acaaaccatg gcaccatccc cggaagcacg ctggcactgt agacgtagac
    21961 aaacacacgc tgcggattgg gaatgatgta gttcaggcat gagccaatta acaggatagc
    22021 aatagatact gccacacccg caaccggtac gccgttacgg gaaactttcg ccatcgccgt
    22081 cggtaactga cggtttttcg ccagtgcgta gagcatacgt ccgcaactgt acatgccgct
    22141 gttacagcca gagagcgcag ccgtcagcac cacaaagttg ataatgcccg ccgctgcggt
    22201 aatgccgatt ttggcgaaag tcagtacgaa cgggctaccg ttgctgccta tttcattcca
    22261 cgggaagata gtaacgataa cgaaaatcgc gcctacgtag aaaatcagaa tcctccacag
    22321 cactttgcct actgcactgc gcagcgtcac ctgcggattc ttcgcttcac cagcggtaat
    22381 gccaatcagc tccacgccct ggtaggacgc caccacaata cacaatgcgg tcaggaaccc
    22441 tttccagccc cccgcaaaga aaccgccatg ctctgtgagg ttgctaaaac caatcgactg
    22501 cccgccattg ccaaagccaa agaaaatcac gcccaggcca atgacaatca tcacgataat
    22561 cgtggtgact ttgatcatcg cgaaccagaa ctcgatttcg ccgtacaacc gcaccgccgc
    22621 cagattcgcc aacgccacca gcgccaccgc gatcaatgcg ggtatccact gcgccatctc
    22681 cgggaaccag aactggacat aaacgccaat ggcggtgatt tctgagatcc ccaccgccat
    22741 ccacataaac cagtaagacc aggcggtgag atagccaaag aacgggctca tataacgatg
    22801 cgcataaacg gcgaacgaac cggtaaccgg ttcgaggaat aacatttcgc ccattgaacg
    22861 catgatgaaa aagacgaaca gcccggcgat gatataggcc aacaatacgg atggcccggc
    22921 ccatttcagg gtactggcgg cccccataaa caggccgacg ccaatggtgc cccccagggc
    22981 gatgagttcg atatgtcgag cttccagccc acgctgtagc tctggtttgt tatctgccat
    23041 aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg gttatcgtta tgggtacatc
    23101 gagtgttgcg aatgttttcg taattcagga gaaatggcaa ataaagcatt aaaaatttga
    23161 atgctttgtg taataaaaaa gcaggaaggc gacgaagtga ccactccgtc gctttacaga
    23221 gagaggaaaa tcagaggttg ccggtgtaat gccagcgtaa ataacgcagc aaacgaagct
    23281 gacgcttaat gcggctcggc tgcgaaagca ggcggtagag ccactccagc cccagcgttt
    23341 gccagatttt cggtgcacgt tttacgtgac cggtgaaaac atcataagtc ccgccaacgc
    23401 ccatatacag cgcatctgga tgcaccagac ggcagtcgcg catgaagatc tcctgctttg
    23461 gcgatcccat cgcaacggtg acgatttgcg ctccgctggc atgaatgcgt tcaaacagca
    23521 tctgacgctg ctcgggttta aaataaccat cctgactgcc aacgatattc acattccact
    23581 ggttgcgtag tttagcttca gtttgcgcca gcacttcagg tttaccgccc accagaaata
    23641 ccggcgtccc ttctttgcct gcgcgcgcca tcagctcttc ccagagatcg gccccggcaa
    23701 cgcgggaaac ctgcgcctgc gggtactttt tacgtactga acgtacaacg ctgatgccat
    23761 ccgcatattt aaattcggca gcgttaatta actccctgac ctcggcgtta tcttcaatgg
    23821 tcagcatttt ttcagcatta atggcaacca gcgttccctg cttaagctgc ccgtcagcga
    23881 acagataatc gagggcgtgc tgcatatcac gccaaccgat caactgtaag ccacgcagcg
    23941 tataggtcgg tgccgtggtg ttgttattca ttgttatcct tcaacctgcg tccggagcga
    24001 tgattttgta cgtttatgaa tgagtccggc gctttcaaaa agccagtaca gcagttttgc
    24061 gatcatcaga catgcgccga agaccacgat aaaaaagact acgcgtgaga caaacgaatc
    24121 cagcccttca cgcgccagca cgatcatatt gaagatcgcc ccaaagcaga aactgtgcaa
    24181 tatcgcagct ttatagcgat taggctcgcg gttgcccagc tcatacagcc agtcgaacca
    24241 tttgatgatc agaccaacca cgatcgcccc gagcgggatg aacaacgcgc cgcccatcac
    24301 caccagtgag cctataagcg taggcgagat cgccagcccg gagtggttat tcagcacttc
    24361 ccaggtaaag tagttggcgg agttcagtac catactcggg cgacccggcc acagccagga
    24421 agggataaag acatagaaat cgcggacaat gggagccaga ccctggaagt cgatgttgtc
    24481 gtagttctgc aacagcaatg ccagattctc ccacggcgaa aaggtatcgc gggtgagata
    24541 gagaaacgta tagaacgctt catcaccgct cacattcatt ccatagcgtt ttagcgccag
    24601 ccagaacatg ccaacaatcc ccagcacgcc cgctgccgcc agcatccaca acgaaatcca
    24661 gccgtgaata atgccaataa acaggaagat agcgaatgcg ataatgatat tggcgcgagt
    24721 gccgccgaca atcatataag tcagcaagcc aaaagcgacc gtgctgacga ggaaaaacag
    24781 ccacgctttg ctgtcctggc gcagaaagta gaccaccagc atcgccggga tgaaaaagta
    24841 aaagaagcgt tttaacgcca cgccggagac ttcactggag aagatctgac tgtaggagtt
    24901 aagccggaac agcaaaaatc cgttgtgcat gaagaagatg ccgacgctta ccagcgcgat
    24961 gcccatcagg atcacccacg tgagattggt ctccacgcgg ttcatggtaa acagcggacg
    25021 gcgcggtaca tcggcaacgc gtttgcgtag gcgggttttg taggtgacgt agtaaaccgc
    25081 gtagaagcag cccgcagaaa gcaacgcctg caacaagatt tctggcggcg cgacaccaac
    25141 atcaaagcga aataccagca cgctggtcag cgggaagccg aagaaaaagg tgagcaaaaa
    25201 cagcaatgaa aagaagacat tgaagttaaa acgcacacgg cgaaactcaa accaggtcag
    25261 cgtggcaata aacagcgtgc agagcagcca gacaacaaac aagccactga attgcagcag
    25321 actcatgcga cctccccggc ggcaatcgcc aacgcccgct gccagccttg cagatagttc
    25381 gggctaaaga aggcaatggt gtttttatcc accgacgcca actggcgctg cgcttcacgc
    25441 acaatatcct cgttgagatc gtcggtagta aacagcaccg gcaaatgctg ttccgtcata
    25501 tcctgccaga acggattttc ccggttaagc acacaaggaa tgcccgcctg aatcagtaag
    25561 cacagcgtac caatgccctg ctggcgggca aaaataaagt aaccaagatc gcactgacga
    25621 agtagcgcca gataggcgtc aaattccagt ttttcgttca gaatttgcag attttcttcg
    25681 ctgaataacg ctagccccgc ctgacgaact tcctcgatgt acgcttcgtt attaggcgga
    25741 tatcccatcg gcaccaccac ttttaccgta tcgccaaatt gctgatgaac ggcgcgcaag
    25801 gcagcaatat gctcattgct gcggtcgccg gagttgccca ccagaatggt cattttccct
    25861 tcacgttgcc gatcgttcgc catcgtattg agcgaaggat ccatccgcgt cgggaagtac
    25921 agcagttcgc cccgcacctt tgggtgcgtt ttggcaaaaa agctcaaatc accgcgggtg
    25981 gcaaatacac agccgactcg cttttgcgcc aggcgacgta gtgggtaaaa aagcttatat
    26041 ctcaagccac tggaaagctc atacaggtct gccccccaga tatgccagaa aaactggctg
    26101 ggcttaatgc caccactcag cagagccagc cacagtgtgg gattgaactg accgtggaaa
    26161 aaaaatcgct gctgacggtt agcttttgct ttcgcgatga ccgcttccgc cagcgatttt
    26221 ttcccaggga aaaattgcac agaaagcgcc ggacagctat cacttaagcc gtcgtccttg
    26281 ccaacaacca taaactcgcg cgcgtgatcg ctcgtcgcgg ccagcgcgtc gttgaaaaac
    26341 cgcaaaacgg ttcggttatg gtgagggata tccgatccca gtacgtgaat cagtacagtc
    26401 atgcccgcct acgccagagt aaaaacacgc cacaacaaag agaaaaatag acgatatagg
    26461 ttgccatata ggcctgcgcc gcgcccagtg caccatgcgc agggattagc cagtgggcaa
    26521 ataccatcaa taaagtgaac tggctgactt ccgccagaat ataaaaccgc agtgacgctt
    26581 tggcgatcac cagataacca aagacataag cgcccacttt taacacatca cccaccaact
    26641 gccaggcaaa gagatcgcgc atagcggtaa atttattcga caacagcagc cagatagcaa
    26701 aatcacgcag caaccagacg gtaaaactcg ccgccgccac tgccggtaag acgaatttta
    26761 gcgatttaac cacttcccgg gtgatatcgc gcttttccgt tagccgcgac aacgtgggca
    26821 gcaaatatac gctgaacgat gccgtaataa attgcaggta ggcatcggaa atactgctca
    26881 ccccttgcca gatccccacc tcatcccagc tatactgcgc cgccagcagt ttacgcatca
    26941 tgatgtaagc aacaggcaag gtcaccgacg taatcaacgc catgagcgta aatttgctca
    27001 actgccctgc cagaccgtta tcccagctgg gtttcagata gcttaacggg atgacaccac
    27061 gtttgatcaa catgatggcg gcaggaatta ccaccagcgc gggaatcagc gccagaccca
    27121 gcaacgcccc ttcataaccg cccaaacggt aactgacgta gtacgcgaga acgccaatca
    27181 ggctgccgac aatcagggat aacgcattac ctgcggcatc gcgaaagcct ttcatcaacg
    27241 ccagtaacag gttgccccag gcaatcccca tttgcaccag cgccactaaa cgcaccagtc
    27301 cctgatagtc ggtattacca aacaatccct ggctgattgg cgctgctgcc agcacaaaaa
    27361 ccagcgccat tagcgtagag aagccaagta ccatcgctga tgaagtgccg accacgcggc
    27421 gcagctgttg gggattatca tggtactgag caacgtattt ggttacgcca ttaaagatgc
    27481 cagccccggc aagcacgccg agcacggtaa tcaactggcg gaaatttgcc gccagcccaa
    27541 gccccgccgg accaaatgac accgccagca acttaccgac cagtaacccg gcaccaatct
    27601 tgaccagtgt actggccgcc gtccacaagg acgcttttgc caacgacata tcaggagaag
    27661 tagttcaaca aagtcgcaat taccgtacgc tgattgacgg gcgacaggtt gtagaacagc
    27721 ggcaggcgca gcaggcgctc actctctttg gtggtgtagc gatcttcacc gtggaactca
    27781 ccaaagcgtt cccccgcagg gcaaccgtgc agcggaatgt aatggaacac cgccatgatt
    27841 tccgcttctt tcagaaagtt aatcaacgcg ctccggtcat caatatcccg cagtttaatg
    27901 tagaacatat gcgcgttctg cacgcagcca acgggaatcg acggcagctc gatacgcccg
    27961 gctttcgcca gaggtgctaa cgcatcgtag tagttttgcc acagcgccag acgttgctga
    28021 ttgatacgat ctgctgcttc cagttgcgcc cacaggtacg cagcttgcag atcggacatc
    28081 aaatagctgg agccgatatc gcgccaggta tatttatcga cctgaccacg gaagaactgg
    28141 ctgcggtttg tgcctttttc acggatgatc tcggctcgtt cgatcaacgc tttatcgtta
    28201 atcagcgtcg cgccgccttc accgcccgcc gtgtagtttt tggtttcatg gaagctaaag
    28261 cagccaatat gaccaatggt tcccagtgca cgccctttgt aagtggacat cacgccctga
    28321 gcagcatctt ccaccacaaa cagattatgc tttttcgcca acgccataat ggtgtccatt
    28381 tcgcaggcca cacccgcgta atggaccggc acgataacgc gcgttttgtc ggtgatcgct
    28441 gcttcaatca acgtttcgtc gatgttcatg gtgtccgggc gaacatccac aaaaacgatt
    28501 tttgcgccac gcagcacaaa ggcattggca gtggagacaa aggtgtagct cggcatgatc
    28561 acttcatcgc caggctggat atcgagcagc agcgccgcca tctccagcga agcggtgcag
    28621 gatggcgtca gtaacacttt ggcgctgcca aaacgttgct ccagccactg ctggcagcga
    28681 cgggtaaaac cgccatcgcc acacagtttg ccactaccca ttgccgactg catatagtcg
    28741 agttcggttc ccaccaccgg cggtgcgtta aatggaatca tgtgatcacc tgtataacca
    28801 gtacgcggtg ctttctacat tcgcaccact ttgtatgtat cgttttagcg cggcggtgtt
    28861 gcccatttgg gtcgccaccc gcaaagttgt tttaccgcga gcatacgccc agtttagcgc
    28921 cgtttgcatc agctcagcac ctgcaccgcg tccagccagc aggccaattc gcgcatctgt
    28981 cgcattgagt tcccgtaaag agacatagcc gcgaatagcg ccggacgccg cacgtaaaat
    29041 cagacattga tgatcaaagg tgccgcgcac ggcattttca atccactgtg cataaaagcg
    29101 accgctggcg tcaggcgcat accacggcgc acgaaaacgg ctttgcgcaa atgcggcgct
    29161 ggctaactga cgtaatgcgg gaatatctgt ctcttgtgcc acttctgcgc cgctatcacc
    29221 gacattgttc acgggtagcg ccaaatcaac ttcaccttct accagggaga atcccagctg
    29281 ttgcagggca tccagttcac ccgtatttga tgccgcaatt ttggcctgca cccgtgacca
    29341 cggcgctaac gcgtctggcg tcaggagcgg tgcttcagac gtgatgcgca cgatggcgct
    29401 gttaacacca aagaaggcgt tttcccaggt tagtggctca atactggcgc ggacgggcac
    29461 gaagtaactc cagcagatat tggccgtagc cagttttcgc cagcgagctg gcggcgcgct
    29521 tcacgccttc gtcatcaagc cagccgttac gccaggcgat ctcttccagg caggcaatct
    29581 taaagccctg gcgtttttcc accgtctgta caaaggtgct ggcttcaatc aggctgtcgt
    29641 gagtgccagt atccagccag gcaaatccgc gcccgagcag ttcaacggtc aggttgcccg
    29701 cctcgaggta catctggttg atggaggtaa tctccagttc accacgctcc gacggcttca
    29761 ccagctttgc gtactccacg actttactgt cgtagaaata aagcccggtc accgcccagt
    29821 ttgacttcgg ctgttttggc ttttcttcca gcgagatagc gcggaaattg tcgtcaaact
    29881 ccacgacgcc aaagcgttcc ggatccatca cctgatagcc aaataccgtc gcgccttgcg
    29941 tgcgcgccgc aacatgacgc aacttcggac tgaagccctg tccgaagaag atgttatcgc
    30001 ccagcaccag acaagaaggt tcaccgttga ggaaggtttc accgatgata aaggcctgcg
    30061 ccagaccgtc cggactgggc tgttcggcat attccagctg gataccgaac tcactaccat
    30121 cgcccagcag gcgctggaaa taacctttat cttccggcgt agtgatgatg agaatttcgc
    30181 ggataccggc cagcatcagc accgacagcg gatagtaaat cattggctta tcgtaaatcg
    30241 gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa tcgggtgccg gaaccgcccg
    30301 ccaggataat acctttcatg tttgcctccg ctgaaaatta gcctttcaga cctaaacgct
    30361 cgccctgata gctgccgtcc tgcacctgct tccaccagct ttcattagcc agataccact
    30421 gcaccgtttt acgcattcca ctttcaaagg tttcctgcgg cagccagcca agttcacggg
    30481 caattttcga agcatcaatg gcataacgca gatcatgccc cggacggtca gcgacaaagg
    30541 tgatcaggtc atgatagtgc gccacgccgt gcggcttgtt cggagccagt tcttccagca
    30601 gctcgcaaat ggtttccaca acatcgagat tcttacgctc gttgtgacca ccaatattat
    30661 aggtttcacc gactttcccg gtggtcgcca cgcaatacag cgcgcgggcg tgatcttcca
    30721 catacagcca gtcacggatt tgctgcccgt tgccatatac cggcagcggt ttacccgcca
    30781 gcgcgttgag gatcatcagc gggatcagtt tttccggaaa gtggtaagga ccgtagttat
    30841 tcgagcagtt ggtgataagc gtcggcagac cataggtccg cagccaggcg cgcaccaggt
    30901 ggtcgctgct ggcttttgaa gcggaatagg ggctgctcgg cgcatacggc gtggtttcgg
    30961 tgaagaaatc atccgtcgag tgcaggtcac catatacttc gtcagtggag atgtgatgaa
    31021 aacggaacgc tgatttttta tcttccgtca gcgcattcca gtaagcccgc gccgcttcaa
    31081 gcaatgtata agtcccgaca atgttggttt caacaaacgc tgccgggccg tcaatagaac
    31141 ggtcaacatg gctttcggct gccagatgca tgacacaggc tggctgatgc tcagtgaata
    31201 cgcgcgccag ttctgcccga tcgcagatat caactttctc aaaggcaaag cgctcgcttt
    31261 gcgcgaccgg tgccagcgac atcaggtttc cggcgtaggt cagcttatcg accactacca
    31321 ccgcatcgct cgtttcgttg atgatataac gcaccagcgc cgagccaata aacccggcac
    31381 cacctgttat cagaatcttt ctcatcagcg ccagactcct ttggcatcga cgacatactg
    31441 ctgatggacg ttgtcgccat tgataacttt gaactgacta tgatcgacca gcatcaccag
    31501 cacatctgcc gttgccagcg cctcgtcaag ctgcgccaga gtacaaagcc cggtcagttt
    31561 cttcggcaac tggtggatgt taggctcaac aaccagcgtt tcgccgctgt gccactgggc
    31621 gatcagttca gcgatttcca tcgccgggct ttcgcgcagg tcatcaatat tcggtttaaa
    31681 cgccagacca aagcaggcga ttttcagttc actggcgcgt ttatcggtag ccgccagaca
    31741 atcagccacc gccgctttca cctgatcgat aacccagaac ggtttgtgat cgttcacttc
    31801 gcgcgcggta cggataagcc gcgcctgctg cgggttctgt gccacaataa accacggatc
    31861 aacagcgatg cagtgaccgc ccacgccagg gccaggctga agaatattga cgcgagggtg
    31921 acgattcgcc aggcgaatca gttcccagac gttaatcccc tgatcggcac aaatcagcga
    31981 caattcatta gcaaaagcga tattcacatc gcggaagctg ttttcggtga gcttacacat
    32041 ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg aggaaaatct tgtacagttc
    32101 gctggcgcgg tccgagcaaa ccggcgtcat gccgccaatc acgcgatcgt ttttaatcag
    32161 ctcgaccatt acctgtcccg gcaacacgcg ttccgggcag taggcaatat tgacgtcagc
    32221 ctgctcgccc acctgctgtg ggaaagtgag atctggacgc atctctgcta accattctgc
    32281 catcttctcg gttgacccca ccggcgaggt ggattcaagg atcaccaacg cgcctttttt
    32341 cagcactggc gcaatggagc gagcagccga ttcaacgtag gtcatatctg gctcatgatc
    32401 gcctttaaac ggcgtgggta cagcaatcag ccaggcatcc gcttcaactg gcgtcgtgct
    32461 cgctcgtaaa aaaccgcctt ctacggcagt ttttacgacg ctcgccaaat caggctcgac
    32521 gatatggatt tcgccacgat tgatggtatc aaccgcgtgt tggttgatat cgacaccaat
    32581 cacctgtttt tgccgtgagg caaacgcagc tgctgtaggc agcccgatgt aacccagtcc
    32641 gataacagaa atggtcgcaa aactcatagt gatatccgat tattttttaa cgcttccaga
    32701 atgcgagagc atgcctgacc atcaccatac gggttatggg cgcggctcat agcttgatat
    32761 tcgttttcgt cttttaaaag acgcgtcact tcctcgacga ttcgctgctt atccgtgccg
    32821 accagacgta ccgtacccgc cgtcaccgct tccggacgct cagtggtatc gcgcattacc
    32881 agcaccggtt tccccagcga aggcgcttct tcctgaatgc cgcctgagtc ggtcaaaatc
    32941 agccaggcgt ggttcatcag ccagacaaac ggtaaatact cctggggatc gatcagaatg
    33001 acatttttca catgccccag aatgcgattg accggctctc tgacgttcgg gttgagatgc
    33061 actggataga caatctggat gtcctggtgc gtggtggcga tgtttgccag cgcatggcag
    33121 atttcttcaa agccacgacc gaagctctca cgcctgtgac cggtcaccag aatcattttt
    33181 ttattggggt cgataaacgg gtaatttgcc gccagttctg aacgcagcgt gtcgctgctc
    33241 atcacctggt cacgcaccca taacagtgca tcaataactg tattaccggt aatgaagatt
    33301 cggctgtcag caacattttc acgcagcaag ttttgccggg aagtttcggt tggagagaag
    33361 tgatacatcg ccagatgccc ggtcaatgta cggttagcct cttccggcca cggcgaatag
    33421 agatcgcccg tgcgcagacc agcctcaacg tgaccaacag gaatacgctg ataaaacgcc
    33481 gccaggctgg ttgccagcgt tgtagtagta tcgccgtgaa ccagcacgac gtctggtttg
    33541 aactcggcta gaattggttt tagcccttcc agaatccgac aggttatctc tgtcaggccc
    33601 tgtcctggct gcattatgtt gagatcgtag tcaggtacaa tggaaaagag tttcagcacc
    33661 tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt tagcctcaaa aaaaggatct
    33721 tttgccaacg catgcaccaa cggagccatc ttgatggctt ccgggcgcgt accaaataca
    33781 gtcagtactt tcacatcgat tctcttcgaa taagcggcga gcgcctttgc gctcaccgca
    33841 gcagtgttgc tatttcgagc aacggcgggt taatgcgaca ccagccccga tcagcccccc
    33901 gacaatgccc cacataatca tcaggaaggc acgacgtggg ctatcgcgtt ttaccggttc
    33961 ttccggcgta cgcaaatagc gataggtctg aaaacgcgga tccagggttg gaccaacatt
    34021 cagggtgttt aacatggccc gattctgatc atagtcgaga tcaaaggccg gaccgacggc
    34081 ctgtaaattt tccagtcgag cctggagcat tggacgccca agcaggaaca tttctgaatc
    34141 aggtaattcc tcggcaggca catctgtcgc actgcgcgaa atattatgct gctcagcaat
    34201 tttcagcgcc tgttcaatac tgttcatccg gcggtcgtag atggctttcg ccacctcttc
    34261 ctgacgcttc acctgtgctt tcatctggat ggtacgcgcc gcccatgcgc ctttcagctc
    34321 atcattcaga tggctggctg cacgctggct ggcaaaagca acatactgac gtaacaggtt
    34381 attagcgtca ggcgcggttt cggcaatcag cttaacgctg tcattaaccg cgcgggtgaa
    34441 gtcaccggga ataaacacga tgttgttaat catttcatcc agcaacgccg catcggcttt
    34501 actgttgccc accatccgct gcttgtaata gtcggtttgc agccagaact ctctgcgggt
    34561 atcccacgag gccagttgca taacaaactc tttataggct tcgtccatga ccgatggttg
    34621 gtcggcagaa gccatgtttg aacggacatc caggttacgc aaaaattgct gctgtgagta
    34681 atatcccccc agcatattca ccgttggacg atcggtaatc gccgtcgagc tccactcctg
    34741 acgagcaaaa aacgtatacg ccagcgcgat taacgcaaac gccagcccca tgccaataat
    34801 ccatagcttc ccagcccaca aggtacgaaa caacccacga atatccagtt cattttcagc
    34861 gtcttcggcc ggtttcccag gcattggttg tgtcatcaca tcctcattta tttggttaaa
    34921 ttggggctgc caccacgatt tctacgcagt ctgcgtttta cgcgcttaat aaagcgagca
    34981 actttccagg cacgcttaat gcaatatcca tagaggaaga atgctagcaa aaagagcacc
    35041 agcatgaccc actccgggac aaaatgagaa tattctgcca gcaccccaat ggaggcgagc
    35101 agagctgcgg caagggtaat cagcacaaac gcctggcggg aagtaaaccc ggcacgcatg
    35161 atcaaatggt gaatatgctg acggtcagga gagaatgggc tcatgccttt acgcaggcga
    35221 cggtacataa tcgccaccat atccattagc ggaatggcga ttatccacaa agcggtaacc
    35281 gggctgatgg gatgggtttt gccctgggtc gtttcgagca ggatccagat aacggtaaaa
    35341 ccaatcagcg tactgcccgc atcacccata aagactttgt agcggcgacc caggatacca
    35401 aggttaagca tgatgtatgg caggatggcg gcgatcatcg cgaagcacca gattgcgagg
    35461 ctggtttgcc cgtcgaacca caaaatcata ccgattgctg caaacgagac gcaggacaac
    35521 ccgcccagca agccatcaat gccatcaacc atgttgaacg cattaatggc cgcccagacg
    35581 gcaaatagcg tcaggaagta gccaaacggt ccgagcacca tctcccagga gccaaagata
    35641 taacccaggc tactgagata aagtttgccg aacaccatca taacaatgcc aacagcagcc
    35701 tgtatggtgg cacggatttt tacgctgata tcaaaacggt catccagcgc gccaatgaaa
    35761 acaagcacac cggcacaagc gagatagaga gatgcatgcg gaatatagta atcgacaatt
    35821 ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc caacgagagg tatcaatccc
    35881 tggtgacgtt tgcggaagtt aggtttatcc actaaaccga ctttttttgc caccttacgg
    35941 gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga tgagatcagt actcactgtc
    36001 agtaaattca caatgcatgc tctcagagaa aattattagc agaagtataa ccacgaagac
    36061 cattattcag aagggaaact ctgctctgaa cccaccaacc ttgtgcaaaa aacaaccacg
    36121 tgattcgtgt tatgcgcaat aaacaaacat aactcgtatt tgttataacg ccgttaagat
    36181 tacagatata aaaacaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt
    36241 atgagcg
//
LOCUS       DAOTRK010000033        35748 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.033, whole genome shotgun sequence.
ACCESSION   DAOTRK010000033 DAOTRK010000000
VERSION     DAOTRK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.033"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(164..379)
                     /locus_tag="QIE41_001609"
     CDS             complement(164..379)
                     /locus_tag="QIE41_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530505.1"
                     /translation="MMFSSCEMALVVMRQMLIFWLLSLLLQFTSPQTHLVWYLFALCQ
                     RIIEVKEPANLFRLAASVLLMAMIMNQ"
     gene            378..686
                     /locus_tag="QIE41_001610"
     CDS             378..686
                     /locus_tag="QIE41_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDS3530506.1"
                     /translation="MTVIVYGKSTFAGNAKTRRHERRRKLAIERDSICNIIDSIFGTD
                     SEEPVQEDPRKRLSLSEKAISLGSLRCKKVDECSGSVCLPNVAIYAAGYRKSKQLTAR
                     "
     gene            complement(703..1284)
                     /locus_tag="QIE41_001611"
     CDS             complement(703..1284)
                     /locus_tag="QIE41_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HDS3530507.1"
                     /translation="MNNSWWQELMHFFLQGMTLKQLIHMLIILIILIIVMPVSVKEWI
                     NLHNPEILPHYWMYYILLFCVSYVLNGVVNSAYHAVTERIEVFVAQKRKSKEEKYVQD
                     LFDSLTLGERAYLAFAVAANNQLQTEKGAHESISLLKKGLLVRRPPAVGYPDTDRFVI
                     PESYRHECYIRFAGKADSLMDELIAQDKHGKNK"
     gene            1498..1698
                     /locus_tag="QIE41_001612"
     CDS             1498..1698
                     /locus_tag="QIE41_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HDS3530508.1"
                     /translation="MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIRATAMIARELGKQNNKAA"
     gene            1881..2249
                     /locus_tag="QIE41_001613"
     CDS             1881..2249
                     /locus_tag="QIE41_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HDS3530509.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVYEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            2322..2486
                     /locus_tag="QIE41_001614"
     CDS             2322..2486
                     /locus_tag="QIE41_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HDS3530510.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            2455..2598
                     /gene="kil"
                     /locus_tag="QIE41_001615"
     CDS             2455..2598
                     /gene="kil"
                     /locus_tag="QIE41_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDS3530511.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            2673..2969
                     /locus_tag="QIE41_001616"
     CDS             2673..2969
                     /locus_tag="QIE41_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HDS3530512.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDNDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            2975..3760
                     /gene="bet"
                     /locus_tag="QIE41_001617"
     CDS             2975..3760
                     /gene="bet"
                     /locus_tag="QIE41_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDS3530513.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWND
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            3757..4437
                     /locus_tag="QIE41_001618"
     CDS             3757..4437
                     /locus_tag="QIE41_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDS3530514.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYIASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            4434..4616
                     /locus_tag="QIE41_001619"
     CDS             4434..4616
                     /locus_tag="QIE41_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HDS3530515.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            4589..4780
                     /locus_tag="QIE41_001620"
     CDS             4589..4780
                     /locus_tag="QIE41_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDS3530516.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            4857..5072
                     /locus_tag="QIE41_001621"
     CDS             4857..5072
                     /locus_tag="QIE41_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDS3530517.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            5171..5392
                     /locus_tag="QIE41_001622"
     CDS             5171..5392
                     /locus_tag="QIE41_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDS3530518.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(5603..6205)
                     /locus_tag="QIE41_001623"
     CDS             complement(5603..6205)
                     /locus_tag="QIE41_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530519.1"
                     /translation="MSYFLRKKWMVNLSGSGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQRGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINILGAVVTYAQTARDDTE"
     gene            6448..6615
                     /locus_tag="QIE41_001624"
     CDS             6448..6615
                     /locus_tag="QIE41_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530520.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            6655..6873
                     /locus_tag="QIE41_001625"
     CDS             6655..6873
                     /locus_tag="QIE41_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDS3530521.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            6851..7921
                     /locus_tag="QIE41_001626"
     CDS             6851..7921
                     /locus_tag="QIE41_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDS3530522.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTALHVDALGISMKETLDKCKEILDGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            8056..9339
                     /locus_tag="QIE41_001627"
     CDS             8056..9339
                     /locus_tag="QIE41_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDS3530523.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLESNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGNVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9409..11670)
                     /locus_tag="QIE41_001628"
     CDS             complement(9409..11670)
                     /locus_tag="QIE41_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDS3530524.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGSIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPNFDVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(11853..12182)
                     /locus_tag="QIE41_001629"
                     /pseudo
     CDS             complement(11853..12182)
                     /locus_tag="QIE41_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
     gene            12150..12911
                     /locus_tag="QIE41_001630"
     CDS             12150..12911
                     /locus_tag="QIE41_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HDS3530525.1"
                     /translation="MTHDDAEENNKQKARFRNKWSMESVTNGISVSHDNPDKGQRIQA
                     IPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHPVSAPQNANQVTEITSA
                     APDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLYPALATPSADNSQLVIN
                     ALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPLFAAVYTPEKQPDALYR
                     NLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            12929..13276
                     /locus_tag="QIE41_001631"
     CDS             12929..13276
                     /locus_tag="QIE41_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDS3530526.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            13273..13965
                     /locus_tag="QIE41_001632"
     CDS             13273..13965
                     /locus_tag="QIE41_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HDS3530527.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            13976..14959
                     /gene="traN"
                     /locus_tag="QIE41_001633"
     CDS             13976..14959
                     /gene="traN"
                     /locus_tag="QIE41_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HDS3530528.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNGNMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            14962..16251
                     /gene="traO"
                     /locus_tag="QIE41_001634"
     CDS             14962..16251
                     /gene="traO"
                     /locus_tag="QIE41_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HDS3530529.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPATKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            16251..16955
                     /gene="traP"
                     /locus_tag="QIE41_001635"
     CDS             16251..16955
                     /gene="traP"
                     /locus_tag="QIE41_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HDS3530530.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPSFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            16955..17482
                     /gene="traQ"
                     /locus_tag="QIE41_001636"
     CDS             16955..17482
                     /gene="traQ"
                     /locus_tag="QIE41_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HDS3530531.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            17533..17937
                     /gene="traR"
                     /locus_tag="QIE41_001637"
     CDS             17533..17937
                     /gene="traR"
                     /locus_tag="QIE41_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HDS3530532.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKYLLLIPMMLLPKA
                     VLADGDLADMVRNVGEGAKTAQSSSITIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            18001..18189
                     /gene="traS"
                     /locus_tag="QIE41_001638"
     CDS             18001..18189
                     /gene="traS"
                     /locus_tag="QIE41_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HDS3530533.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            18173..18973
                     /gene="traT"
                     /locus_tag="QIE41_001639"
     CDS             18173..18973
                     /gene="traT"
                     /locus_tag="QIE41_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HDS3530534.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            19063..22107
                     /gene="traU"
                     /locus_tag="QIE41_001640"
     CDS             19063..22107
                     /gene="traU"
                     /locus_tag="QIE41_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HDS3530535.1"
                     /translation="MNINRFIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISCVFERDPEMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEDLVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEDLASELIRKQGYNL"
     gene            22107..22721
                     /gene="traV"
                     /locus_tag="QIE41_001641"
     CDS             22107..22721
                     /gene="traV"
                     /locus_tag="QIE41_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HDS3530536.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRLFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            22688..23890
                     /gene="traW"
                     /locus_tag="QIE41_001642"
     CDS             22688..23890
                     /gene="traW"
                     /locus_tag="QIE41_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HDS3530537.1"
                     /translation="MQRKTLLAALITTLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     DESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            23919..24503
                     /gene="traX"
                     /locus_tag="QIE41_001643"
     CDS             23919..24503
                     /gene="traX"
                     /locus_tag="QIE41_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDS3530538.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINILVPLMETRLITNS
                     VQHMLKQQKSRLEKFRQVNNKKAQLCLSWEEALLASHMSVDDLDRRFRRRRTAWRLCC
                     WSLLAIALFLSGMLFAASSLPLMTLVRAISTLMLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            24600..26768
                     /locus_tag="QIE41_001644"
     CDS             24600..26768
                     /locus_tag="QIE41_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDS3530539.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQKAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYR
                     TQLQNSSYTPPLGTVVSSSEQRMANAQDPKSALSEIMGPLVSLTNRIATETSGTGTTS
                     AQINPLIKMKNIGDITMVSAEGIWTIYTTARVVVAGGKDSILVKFFNSLTGAASMLTA
                     LFEALAPPVYFLLFLMFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTTVAAIFALQAYLPDHVINFLGGRDGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDNTKGSDDKDGIKG"
     gene            26839..27501
                     /gene="excA"
                     /locus_tag="QIE41_001645"
     CDS             26839..27501
                     /gene="excA"
                     /locus_tag="QIE41_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HDS3530540.1"
                     /translation="MKAKRETTDRFPTWWLFYYVLRKAYFFLGIPFFLFCALGFTEML
                     CSDRYFGNKVEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGIPVAE
                     VF"
     gene            complement(27573..27782)
                     /locus_tag="QIE41_001646"
     CDS             complement(27573..27782)
                     /locus_tag="QIE41_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDS3530541.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            28174..28350
                     /locus_tag="QIE41_001647"
     CDS             28174..28350
                     /locus_tag="QIE41_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530542.1"
                     /translation="MNLVDAFVKKVISGPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(28415..28711)
                     /locus_tag="QIE41_001648"
     CDS             complement(28415..28711)
                     /locus_tag="QIE41_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530543.1"
                     /translation="MSALRHVLSDILFSGKNRLQVLAKTSGGAGFGALVGEELSITDT
                     IETVVTGSVRANNGVLSPVMEKRMINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            29011..29262
                     /locus_tag="QIE41_001649"
     CDS             29011..29262
                     /locus_tag="QIE41_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530544.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(29334..29486)
                     /locus_tag="QIE41_001650"
     CDS             complement(29334..29486)
                     /locus_tag="QIE41_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDS3530545.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            29801..30892
                     /locus_tag="QIE41_001651"
     CDS             29801..30892
                     /locus_tag="QIE41_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein finQ"
                     /protein_id="HDS3530546.1"
                     /translation="MEMSQLKQPIFLKKIKKVINTIPGLEEQIFACRNKKRSDNPLLF
                     IDRKDEERILMSRLQSQQKNEELASKLESLFHGNELSSPHSILCFIYWRYTKKIYRLS
                     EDIISDVANTYVDNIPAQILKELPSWSIYVSAENLHTILPTSYPIHGFFFYPFLDNNG
                     NIIRLFIIDDLKQSQGTTGLKEKDVDVVNNIIRIKDSREGLLDSRKMECIDGEVVVTV
                     NEKLKDFRDREFNLLNAQISMVLYICSQINDIKEKNQFKRSEKHKKHVHTHHELPAQN
                     IREWDVGIRMGQAIRQYRQAEPTGKERTTIGSKRPHIRRGHWHTYWTGSKKPELAHER
                     KPRLIWLPPVPVNLEDVNKLPVVITPIDK"
     gene            31199..32407
                     /gene="trbA"
                     /locus_tag="QIE41_001652"
     CDS             31199..32407
                     /gene="trbA"
                     /locus_tag="QIE41_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HDS3530547.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLEDGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTEDIL
                     PRTPATDNEF"
     gene            32426..33496
                     /gene="trbB"
                     /locus_tag="QIE41_001653"
     CDS             32426..33496
                     /gene="trbB"
                     /locus_tag="QIE41_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HDS3530548.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPQDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLYAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            33489..>35748
                     /locus_tag="QIE41_001654"
     CDS             33489..>35748
                     /locus_tag="QIE41_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HDS3530549.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILTAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKK"
BASE COUNT         9995 a         8784 c         8517 g         8452 t
ORIGIN      
        1 catgtgctaa cccacgaact aacacgccag caaacaattc tttctctcct actgaccaat
       61 cttcaatctt tacaaaaata aattccttta catatcaaaa acatatctca ttaagttaat
      121 gaaccacaaa caattcgtac ttatagttct tgataatatc gaactattgg ttcattatca
      181 tcgccatcag caggacgctg gcagccaaac ggaacagatt ggcaggctct ttaacttcga
      241 tgatgcgctg acaaagcgcg aacagatacc aaacgagatg ggtttggggt gatgtgaatt
      301 gcagcagtaa cgacagcaac cagaagatca gcatctggcg catcaccacc aaagccattt
      361 cacatgagga aaacatcatg acggtaatcg tgtacggaaa atcaacattt gcaggaaatg
      421 ccaaaactcg ccgtcatgag cggcgcagaa agctggccat cgagcgtgat tccatctgca
      481 acatcatcga ttcgatcttc ggcacagaca gtgaggaacc tgttcaggaa gacccgagaa
      541 agcggttaag cctttctgaa aaagcaatat cactcggaag ccttcgctgc aagaaagtag
      601 atgaatgcag tggaagtgtt tgcctgccaa acgtagctat ttacgcggca ggctaccgga
      661 aatcaaaaca actgacggcg agatgataaa ttcatttgct aattacttgt ttttgccatg
      721 cttatcctga gcgataagtt catccataag gctgtctgcc ttcccggcaa accgaatgta
      781 gcactcatgt ctatagcttt caggaataac aaaacggtcg gtatcaggat atccaacagc
      841 aggaggcctt cgaacgagga gtcctttttt gagcaatgaa attgattcat gagctccctt
      901 ttccgtttgt agctggttat tagcggctac agcgaatgcc aaatacgctc tttctccaag
      961 agttaacgaa tcaaacaaat cttgcacata tttttcttct ttagatttgc gcttctgagc
     1021 aacgaatacc tcaattcttt cagtcacagc gtgataagcg gaattaacaa cgccgttaag
     1081 cacatagcta acgcaaaaca acaggatgta atacatccag taatgaggaa ggatttctgg
     1141 attatgcagg tttatccatt cttttacgct taccggcata acaataatca atatgatcag
     1201 gatgattagc atatgaatca actgtttaag tgtcattcct tgcaggaaaa aatgcattag
     1261 ttcctgccac catgagttgt tcatcggcgt ttctcttttg ctctctgtag gggtgaatag
     1321 agtttatccg atttctcgct gtaggggtac acgagaacca ccgagcctga tgtggttaaa
     1381 agacaggcac aatctttact accgcaatcc actatttaag gtgatatatg gaagaagaat
     1441 ttgaagagtt cgaagagcat ccccaggatg tgatggaaca ataccaggac tatccttatg
     1501 actacgacta ttgataaaaa tcaatggtgt ggacaattca agcgatgcaa tggatgcaag
     1561 ctgcaatcgg aatgcatggt taagcctgaa gaaatgtttc ctgtaatgga agatgggaaa
     1621 tatgtcgata aatgggcaat acgagcgacg gcaatgattg ccagagaact tggtaaacag
     1681 aacaacaaag ctgcctgata gtggccttta tttttggcat aaataacaga ataaacactg
     1741 cactgtgtat tcattccaac gagtgaatac acggagcaat gtcgctcgta actaaacagg
     1801 agccgacttg ttctgattat tggaaatctt ctttgccctc caatgtgagg gcgatttttt
     1861 atctgtgagg atatgaatag atatcaaaca tcaaaaaata catcattgat tacgactgga
     1921 aagcatcaat agaaattgaa atcgaccatg acgtaatgac agaggaaaaa cttcaccaga
     1981 ttaataattt ctggtcagac tctgaatacc gactcaataa acacggctct gtattaaatg
     2041 ctgtattaat catgctggcg caacatgctc tgcttatagc aatttcaagc gacttaaatg
     2101 catatggtgt tgtgtatgag ttcgactgga atgatggaaa tggtcaggaa ggatggcctc
     2161 caatggatgg tagcgaagga ataagaatta ccgatatcga tacatcagga atatttgatt
     2221 cagatgatat gactatcaag gccgcctgag tgcggcttta ccgtatacca ataacgcttc
     2281 actcgaggcg tttttcgtta tgtataaata aggagcacac catgcaatat gccattgcag
     2341 ggtggcctgt tgctggctgc ccttccgaat ctttacttga acgaattacc cgtaaattac
     2401 gtgacggatg gaaacgcctt atcgacatac ttaatcagcc aggagtccca aagaatggat
     2461 caaacactta tggctatcca gactaaattc actatcgcca cttttattgg cgatgaaaag
     2521 atgtttcgtg aagccgtcga cgcttataaa aaatggatat taatactgaa actgagatca
     2581 agcaaaagca ttcactaacc ccctttcctg ttttcctaat cagcccggca tttcgcgggc
     2641 gatattttca cagctatttc aggagttcag ccatgaacgc ttattacatt caggatcgtc
     2701 ttgaggctca gagctgggcg cgtcactacc agcagatcgc ccgtgaagag aaagaggcag
     2761 aactggcaga cgacatggaa aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg
     2821 atcatttgca acgccacggg gccagcaaaa aagccattac ccgtgcgttt gataacgatg
     2881 ttgagtttca ggagcgcatg gcagaacaca tccggtacat ggttgaaacc attgctcacc
     2941 atcaggttga tattgattca gaggtataaa acggatgagt acagcactcg caacgctggc
     3001 tgggaagctg gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac
     3061 tcttcgccag acggcattta aaggtgatgc cagcgatgcg cagttcatcg cattgctgat
     3121 cgtcgccaac cagtacggtc ttaatccgtg gacgaaagaa atttacgcct tccctgataa
     3181 gcagaacggc atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaacgaaaa
     3241 ccagcagttt gacggcatgg actttgaaca ggacaacgaa tcctgcacat gccggattta
     3301 ccgcaaagac cgcaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga
     3361 accattcaaa acccgcgaag gcagagaaat cacggggccg tggcagtcgc atcccaaacg
     3421 gatgttacgg cataaagcca tgattcagtg tgcccgtctc gccttcggat ttgctggtat
     3481 ctatgacaag gatgaagccg agcgcattgt cgaaaatacc gcatacactg cagaacgtca
     3541 gccggaacgc gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct
     3601 gatcgccctg gataaaacat ggaatgacga cttattgccg ctctgttccc agatatttcg
     3661 ccgcgacatt cgcgcatcgt cagaactgac acaggccgaa gcagtgaaag ctcttggatt
     3721 cctgaaacag aaagccactg agcagaaggt ggcagcatga caccggacat tatcctgcag
     3781 cgtaccggga tcgacgtgag agctgtcgaa cagggggatg atgcatggca caaattacgg
     3841 ctcggcgtca tcaccgcttc agaagttcac aacgtgatag ccaagccccg ctcaggaaag
     3901 aagtggcctg acatgaaaat gtcctacttc cacaccctgc tggctgaggt ttgcaccggt
     3961 gtggctccgg aagttaatgc taaggcgctg gcatggggaa aacagtacga gaacgacgcc
     4021 agaactctgt ttgagttcac ttccggcgta aatgttattg aatccccgat catctatcgc
     4081 gacgaaagta tgcgcaccgc ctgctctccc gatggtttat gcagtgacgg caatggcctt
     4141 gagctgaaat gcccgtttac ctcccgggat ttcatgaaat tccggctcgg tggtttcgag
     4201 gccataaagt cggcttacat ggcccaggtg cagtacagca tgtgggtgac gcgaaaagat
     4261 gcctggtact ttgccaacta tgacccgcgc atgaagcgtg aaggcctgca ttatgtcgtg
     4321 attgagcgga atgaaaagta tatagcgagt tttgacgaga tggtgccgga gttcatcgaa
     4381 aaaatggacg aggcactggc tgaaattggt tttgtatttg gggagcaatg gcgatgaaac
     4441 atcctcacga taatatccgc gtaggcacga tcactttcgt ctactccgtt acgaagcgag
     4501 gctgggtatt tcccggcctt tctgttatcc gaaatcccct gaaagcacag cggctggctg
     4561 aggagataaa taataaacgg ggagctgtat gcacaaagca tctcccgttg agttaagaac
     4621 gagtatcgag atggcacata gcctcgctca aattggagtc aggtttgtgc caataccagt
     4681 agaaacagac gaagaatttc atacgttagc cgcatccctt tcacaaaagc tggaaatgat
     4741 ggtggcgaaa gcagaagcag atgagagaga ccaggtatga caaccacgga atgcattttt
     4801 ctggcagcgg gcttcatatt ctgtgtgctt atgcttgccg acatgggact tgttcaatga
     4861 cacctcagca ggaaaacgcc cttcgcagca ttgcccgtca ggctaattct gaaatcaaaa
     4921 aagccagaca gcagtttccg gataaaaacg tcgatgacat ttgccgtagc gtactgaaga
     4981 agcaccgcga aacggtaacg ctgatgggat tcacaccgac tcatttaagc ctggcaatcg
     5041 gcatgttaaa cggcgtcttt aaggaacgat gaacatgaaa agcaaaatca tcagggagct
     5101 acaggctcct tttttattat tcgcattcac cctcaagcgt attaaccaac aattcaggga
     5161 ttaatggaag atggcagaca tcattgattc agcatcagaa attgaagaat tacagcgcaa
     5221 cacagcaata aaaatgcgcc gcctgaacca ccaggctata tctgccactc attgttgtga
     5281 gtgtggcgat ccgatagatg aacgaagacg cctggtcgtt cagggttgtc ggacttgtgc
     5341 aagttgccag gaggatctgg agcttatcag taaacagaga ggttcgaagt gagcgaaatt
     5401 aactagaagc caaagataaa atcatcgctg agcaggagaa aatcgctaac ggagaaaaga
     5461 cagtaagtca gtatatgaaa accgcatgat atcatcagat aaaaatcggt cgtaaagcga
     5521 aatattaata ccagaacaaa cgagtcgagg taaattatat tacctcgata aattaactaa
     5581 aacttgcccg ctatatacta tatcattcag tatcatcacg cgcggtctgt gcatatgtca
     5641 ctaccgcacc taatatatta attttctttt caacatagat aatattatcg tactcataat
     5701 tgccatacgg atagcaaatg cgaatattct catgtagatc ggggtcatcc acctcagctc
     5761 cagaacaact ttttgaacta ccggaagtat accgatacgg tgcaacataa gacgatgtct
     5821 ctccaggcaa aaaataagtt agtgtcgtaa ggggtataat cagaaaaaat cctgcaaata
     5881 tgcacatccc tgcataaacc ttaaggtatg ctgacagact cttccagcct ctttgtttta
     5941 ctatcccctt cttaacccaa aacagagata acagaaaagc tattcccatg ctaaacagaa
     6001 tgtaatagtg ggatatactc tgattaagaa acgtgaccct gtagatatct gcccgccacc
     6061 agaagaaaag gaaaataaag atcagccctg aaactgtcat gcaaatcaaa taaggatacg
     6121 aatctttttt catgtttagc gcccataaaa tttttcctga cccggacaaa tttaccatcc
     6181 attttttgcg cagaaaatag ctcattactt actgcacaat aatacacaaa attgcgtaaa
     6241 ttttttgcat ggattttagc tctttcagcc gacatttaag gggtaaatag catttcctaa
     6301 aagcaactgc accaacccaa cagaatgggc taccgcttac gttgagagca aaaaagtgta
     6361 tagcagcaat gaacagcatc ctcgcactga cgaggatttc ttttatctga actcgctacg
     6421 gcgggttttg ttttatggcg atgataaatg cacttccgag tcacaggaga atggaatgga
     6481 gagccattca acagagttat cgaagcggag aacatcaacg actgctacga ccactggatg
     6541 atatgggcgc agatagcaca tgcagacata accaatattc gaattgaaga actgaaagaa
     6601 caccaagccg cctgatggcg gttttttctt gcgtgtaatt gcggagactt tgcgatgtac
     6661 ttgacacttc aggagtggaa cgcacgccag cgacgcccaa gaagccttga aacagttcgt
     6721 cgatgggtgc gcgaatgcag gatattccct cctccggtta aggatggaag agaatatctg
     6781 ttccacgaat cagcggtaaa ggttgactta aatcgaccag taacaggtag ccttttgaag
     6841 aggatcagaa atgggaagaa ggcgaagtca tgagcgccgg gatttacccc ctaaccttta
     6901 tataagaaac aatggatatt actgctacag ggacccaagg acgggtaaag agtttggatt
     6961 aggcagagac aggcgaatcg caatcactga agccatacag gccaacattg agttattttc
     7021 aggacacaaa cacaagcctc tgacagcgag aatcaacagt gataattccg ttacgttaca
     7081 ttcatggctt gatcgctacg aaaaaatcct ggccagcaga ggaatcaagc agaagacact
     7141 cataaattac atgagcaaaa ttaaagcaat aaggaggggg ctgcctgatg ctccacttga
     7201 agacatcacc acaaaagaaa ttgcggcaat gctcaatgga tacatagacg agggcaaggc
     7261 ggcgtcagcc aagttaatca gatcaacact gagcgatgca ttccgagagg caatagctga
     7321 aggccatata acaacaaacc ctgtcgctgc cactcgcgca gcaaaatcag aggtaaggag
     7381 atcaagactt acggctgacg aatacctgaa aatttatcaa gcagcagaat catcaccatg
     7441 ttggctcaga cttgcaatgg aactggctgt tgttaccggg caacgagttg gtgatttatg
     7501 cgaaatgaag tggtctgata tcgtagatgg atatctttat gtcgagcaaa gcaaaacagg
     7561 cgtaaaaatt gccatcccaa cagcattgca tgttgatgct ctcggaatat caatgaagga
     7621 aacacttgat aaatgcaaag agattcttga cggagaaacc ataattgcat ctactcgtcg
     7681 tgaaccgctt tcatccggca cagtatcaag gtattttatg cgcgcacgaa aagcatcagg
     7741 tctttccttc gaaggggatc cgcctacctt tcacgagttg cgcagtttgt ctgcaagact
     7801 ctatgagaag cagataagcg ataagtttgc tcaacatctt ctcgggcata agtcggacac
     7861 catggcatca cagtatcgtg atgacagagg cagggagtgg gacaaaattg aaatcaaata
     7921 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
     7981 tttataatgc caacttagta taaaaaagca ggcttcaacg gattcatttt tctatttcat
     8041 agcccggagc aacctgtgaa cacattttca gtttcccgtc tggcgctggc attggctttt
     8101 ggcgtgacgc tgaccgcctg tagctcaaca ccgcccgatc aacgtccttc tgatcaaacc
     8161 gcgcctggta cctcttctcg cccgattctg tcggcaaaag aagcgcagaa tttcgatgct
     8221 caacactatt ttgcatccct gacaccaggt gctgcagcgt ggaatccttc cccgattacc
     8281 ctgcctgcac aacctgactt tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc
     8341 acgattcagg cggcggtaga tgcggcaatt atcaagcgta ccaacaagcg ccagtatatt
     8401 gccgtgatgc ctggtgagta tcagggaacg gtgtatgtcc ctgccgctcc gggtggaatt
     8461 actctgtacg gtacaggtga aaaaccgatt gatgtgaaaa ttgggctttc ccttgatggg
     8521 ggcatgagcc cagccgactg gcgtcacgac gtcaacccac gcggcaaata tatgccgggt
     8581 aaaccagcgt ggtatatgta cgatagctgc cagagcaaac gcagcgacag cattggcgtg
     8641 ctctgttctg cggtcttctg gtcacaaaac aatggcctgc aactgcaaaa cctgaccatc
     8701 gaaaacacac tgggcgatag cgtagatgcg ggtaaccatc cggcggtggc actgcgtact
     8761 gatggcgaca aagtgcagat caataacgtc aatattctcg gtcgccagaa taccttcttt
     8821 gtcaccaaca gcggtgtgca aaaccgtctg gaaagcaacc gtcagccgcg taccctggtg
     8881 actaacagct acattgaagg ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc
     8941 gataacaccg aattccgcgt ggtgaactcc cgtacccagc aagaagcgta tgtgtttgca
     9001 ccggctacgc tgtccaacat ttactacggt ttcctcgccg taaacagccg tttcaatgct
     9061 tccggggatg gcgtggcgca actgggccgc tcgctggatg ttgatgccaa taccaacggt
     9121 caggtagtga tccgtgatag cgccatcaac gaaggtttta acacggcgaa accgtgggcc
     9181 gatgcggtga tttccaatcg tccgtttgcg ggtaatactg gcaacgttga tgataacgac
     9241 gaagtacagc gcaatctgaa tgacactaac tacaaccgca tgtgggaata caataaccgc
     9301 ggcgtgggta gcaaagtggt tgcagaggcg aagaagtaaa aaagaaaacc cgatagtctg
     9361 aaccatcggg ttaatgaaac tgggcgctat atttcatagc gccttttttt acatctgacg
     9421 gtttttattg aagttaatca aactacccgc cttgataatc tcgcgctctt cggccgtcag
     9481 gctttccatg taaagcgtaa tttccgttac cggcgcatct tcatggatca cataaccttt
     9541 aaacgtcgta cccggattat ccagcgctgc tttaatgcca gggatgtaaa tgtaatcacc
     9601 tacgtcaaag ttcggtgctt ccgccatctg cagcggtaac atcccccagt tgatgacgtt
     9661 agaacgatag cgtttagtcg cgtactcctc ggcaatattc gccagaccgc caatcacacg
     9721 ctgacaactc gccgcctgtt cacgcgcaga cccatcgcct ggtttcaccg cataaaccat
     9781 gctgccaatt tcagtttgca gcggatcaat atgctcctga ccagcaatct gcttaatgcg
     9841 cgcaaacacc tctgtcagct cgctgacatt tcccgccaga cgctgatttt ccagctcagc
     9901 agtcgcttta cttctaccaa cataacctgg atcgcggcgt gacagggtaa actccgccag
     9961 accaatcgga tttgaacgat aagaagaggt ttcaccggaa ggaatcagtt cgtcggtggt
    10021 ggtcacttcg tcgaggatct tcgagcacac tttgaggacg atattgtcag tcagcgcacc
    10081 caattctggc cagtctttaa tgttcggtcc gtaaatcagc ggttgctggg tcgccccttt
    10141 cacaaagccc tgataaacac ggtttttata cggcgttaca tcgaaggcgt actccggcac
    10201 gttgtcccag caatccagct cgctggcaga ggttaaatag ccaccgtttg ccgcagtcgc
    10261 cgcaatagaa cgagcgtcca tcaacgccac cgctgacatc tgcccattag ctggcttaga
    10321 gccttcgcgg ttcgggaagt tacgcgtggt gtggcgaata ctcaaaccgt tgttgattgg
    10381 cgtatcgccc gcgccaaagc atgggccgca gaacgcggtt ctgatgattg agcctgcgcc
    10441 aatcaaatct gcgaccacac cttttttggc gagatccata aacaccggct gtgatgacgg
    10501 gtaaaccgcc agcgagaagg tgtcattgcc acaggattga ccgcgcagtg cattcgccgc
    10561 cgcgatgacg ttttcgtagt taccgccaga acagcccgcg ataatcccct gctgcacttt
    10621 caggcgaccg ttttcgactt tatccagcag cgagagtttg gctttaccgt gcgccacgcg
    10681 ttcggactca atttcaatct cacgcagaat gtcggtcagg ttctggttca gcgtgtcgat
    10741 ttcatagacg ttgctcgggt ggaacggcag cgcaatcatt ggtttgatgg cgcttaaatc
    10801 aacgctgatg cagccatcgt agtacgccat tggttgaggg ttaagctggc agtaatcctg
    10861 gccgcgaccg tgcaacgcca gccagttatg gacttcttca tcggtttgcc agacagaact
    10921 taaacaggtc gtttcagtgg tcatcacgtc aacgctgttg cggaaatcgg tagagagcgc
    10981 tgcaacaccg ggacctacga actccatcac tttgtttttg acgtaaccgt ttttgaacac
    11041 ggcaccgatg atagccagcg ccacatcctg cggccccaca tacggcgcag gttttccggt
    11101 cagatgcacc gcaaccacgc ccggatagtc gatatcccag gtgtcattaa gcagttgttt
    11161 taccaactcg ccgccgccct cgccaaccgc cattgtccct aacgcaccgt aacgggtatg
    11221 gctgtctgac ccgaggatca ttttgccgcc gcctgccatc atctcacgca tatattgatg
    11281 gatgaccgca atgtgcggag gcacaaaaat accgccataa cgctgggctg ccgataaacc
    11341 aaaaacatga tcatcaccgt taatagtgcc gccgacggcg cagagtgagt tatggcagtt
    11401 ggtcagcaca tacggcagcg ggaaacgttc cataccggac gctttagcgg tctgtacaat
    11461 accaacaaag gtaatatcgt gagaggctaa tgaatcaaac ttaattttaa gtttatccat
    11521 atttccggac gtattatgag aagagagaat agaccaggca atagtgcctt ttttggcttc
    11581 ttctttttta atttcgccgg tgaaatgttc ttcggcaatt atttcgttat tactggcgag
    11641 aaacacgcct ttttcagata acttgatcat caaatgctcc agatatctgc gcaccggaaa
    11701 gtgcgcagaa agataataaa tggatatact ctaaataatt cgagttgcag gaaggcgaca
    11761 agagagtgaa tccccaggcg cttacataag taagtgactg gggtgagcga acgcagacgc
    11821 agcacatgca acttgaagta tgacgagtat atttacagca tgttccagcc gatcagcata
    11881 ttccaccacc acacgccgag ggtgatatgc accaggaagg ttaatatcgt cagtaccgca
    11941 ccgaccaacc accaggattt aatatcgttg taacccacgc caaagatgac cggacctgcc
    12001 gcgccgccat agtgagtaac catgccgcca taggagttag agaacaacag tgccagcgcg
    12061 gttaacatta acggtgcgcc ggagacattc gccagcatgg caaataccgg taacatggca
    12121 acgatatagg cactaccaga agcgaagaaa taacgcacga tgatgctgag gaaaataata
    12181 agcaaaaagc acgtttccgg aataagtggt ccatggagtc agttaccaac gggatctcgg
    12241 tatcacatga taatccggat aaaggtcagc gtattcaggc gattccggtc gaaaccttta
    12301 tgaaagtggc tgcgcaaatc agccaggggt ggcccgaaga gatgaaagcg ttacgtatgc
    12361 cagaaaacgt cggctcacat ctgtttatcg gtgaagaccg acatccggtc tccgcgccac
    12421 agaacgcaaa tcaggtaaca gaaataacca gcgcagcccc tgacaagctg acacctgttc
    12481 tgggatcagt ggataaggat acgcgtgaac tcaacctgct acttgtccag agtgccgatg
    12541 aacatctgca gggtgtggtc cggctgaacg gcacattgta tccggcactg gccaccccat
    12601 cagccgacaa cagtcagctg gttatcaatg cgctgaccga caaaggttta cgctttgcag
    12661 gttatggtga agcggtaaac cacgatgctg acagcacgaa tcgccccgca ccggagctta
    12721 tgcagtttca tctgaaaacc cgggaagaac cgctttttgc tgctgtctac acgccggaaa
    12781 aacaacctga tgcgttgtac cggaacctgg gcttcgagca gtcatggcag cagtggtcca
    12841 acagccaaaa gccggaggac aggcaggaaa aaactctgca tcaggatctc tcccacagcc
    12901 ccggccgatg agccggtaag gaagccatat gaacatgcat aagtctctgg ttgccgtgct
    12961 cggccttatc ctgatttcat ccacggcaag tgctgcaacc tgtcgtgcgg gtagcgcggc
    13021 acaggccggt agtaatacag gatatgagcg agcacgtgca gctgcggatg catggtcaca
    13081 acgtgaaaac gatgtttcct catccctgca gtcctgtctg tcacgaatca aaaaaatcag
    13141 cataaacctc ccccagttcc ccagccttga cgacattctg tcgcagctgg aaaaccaggt
    13201 ctgcgacgcg gtcgtggaca aggtgaatga aaagctgccg gggaatattg atccctggaa
    13261 ggactacaac ctatgaccga aaataccgag ccatcctcgc catcacatcc ggcacctgac
    13321 ccggaaatac tgaagagcac aatcagggct atgaagcagt cagaacagcg ggcaaataat
    13381 gtccctgcgc tgataaaagc gcttttatgt acgggcacat gtcttcttat cagtattact
    13441 ggtaacgcca ttcagtactg gcactcaacg aatgtggagc gcgaatattt cgctacagac
    13501 aatggtcgtc tggtcaggct ggcaccaaca agtcagccag catggagtca gaatgatgca
    13561 atggcctttg gcagccaggc actggcgacc gcattcaacc tggattttgt gcactatcgt
    13621 tcacagatct cctccctgtc tccgcgtttt tctgacgaag gttttgtcgg atatgtgaat
    13681 gcattacagg catctaacat tctggagacg atcaagaaag agaagatgaa tctgaccgcc
    13741 acgacaggtg ctggcgttct ggtacgacag ggacaaatgt ccgacggcgt atggttctgg
    13801 acattccagt atcctgtccg tatgcgtctg gtcggccaga caaccagcaa gccagaacag
    13861 tcttttgttt tcgaaatcac catccagcgt gttgacccca gactgaaacc atccggtatg
    13921 gaaatccggc agatgatctc ccgtaacgcc ggaccgaact cctgagataa gtaatatgca
    13981 atcacgttat ttactgagca cactgctcct ggtgtgcagc gccgccacgt ctgccgataa
    14041 cgcaggatgg cagaacgcac gcacaccaca aacgacaaat tctgccagcg atcacgttca
    14101 gaatgcctcc caaaacaccg gaggtgttcc ggcaacactg gtaaaaggtg agctgcctgc
    14161 ccccgggcag gccagcccgc tggtacagga tgcggcaaga cttgattctg agctctccgc
    14221 cgatgaaatt cgctcactac gcagtctcat ggcggataac gagcgggcca tcaatgcgcc
    14281 catcaccagt gttgtacccc gtatcagctc tctgacagtt aatctgtccc ccggtgccag
    14341 ccttcctctt gttcgcacag ccatgaacaa cctgagcgtg gtgacattta ccgatatcaa
    14401 cggttcacca tggccacaat ccgatcctcc ttataacgcg gcaccaaaac tctttgatgt
    14461 gcagtacaac gggaacatgg tgacaatcac accattgcgg ccatgggcat ccggaaacat
    14521 ttctgtctac ctgaaaggtc tgtcggtacc ggtcatcctg aacgtaacca gtggcgaaac
    14581 agatacgccg tcatccagtc aggaaatgga cagtcgtctg gatctgcgaa taccacggca
    14641 gggaccaacc agcccggttg tctccatccc gacggataaa atcgccctgc atgatgcaac
    14701 attacaggca tttcttgatg gtattcctcc ccgggaccct tcagttaaga ggctgaaatt
    14761 cacaggtaat gtcccggata caacgatctg gcaacacggc gatgaccttc tggttcgttc
    14821 ccgggcgatg ttgcgcgatg aatttgaaca gaccttgtcc tccgccgacg gaactcatct
    14881 ctggaagctt cccgtcaccc cactactgac tttctctgtg aatggccagt ccgttcacgt
    14941 gacaccagaa ctggagtaat catgagtgct gaacaggatg ccggtaaatc cggaaagaaa
    15001 ctggctgccc tgcttggact gggcagcatc atcttatttg gcggcggcta tatcgcgttt
    15061 tccaaactat cagggagtaa cagcgatatg cagtcagccg tgaacattaa cagtgcagca
    15121 agcggcggaa cgcgcagcgt taccgaaacg cctcattatc gtgaactgtt aagagcggat
    15181 aatgaacgag gggccgcagc tgcagcccgc aataatcaga cgttcattgc cagtcttcca
    15241 caggggcttg atattccgga cactccggaa aagcaacaac agcctgcaac aaaaccggaa
    15301 aactatgctc atcgccaggc ttccggcaca ccacaggagg atcgagcagc cagtgaaaag
    15361 cgtatggagc ggctgcaaaa gctgattgta agaataaaag atcagcatcc tgccggtagt
    15421 acaccaacca tagcaacgac tatgtggaat aaatctccgg cagaaacaac agggcagaat
    15481 ggtacgcagc aattcgcatt acagaatgcc agcctgtcca cgccggtagc agaaaaaggg
    15541 atacaactga tacctgctct gacccgtata ccggcatata ttgatactgc agtggattct
    15601 gataacccgt catccaaggt tatcgcaacc attccggctg gcccctgggc aggggcaacg
    15661 ctgttcagtc cgggagtcaa actggtgggt aacggcgtgg agatccattt cgaccggatg
    15721 agctggaacg gcatggacct taaggtcaat gcatatgcac aacgggaaga caacctgatg
    15781 tcatccgtgg ccagcaatgt gaatacacgc tggtttaaac atatcattct gccgtcagtg
    15841 ttaggaggtg tgggcagtat tggcacactt tataaagatg ccaacacgca ggttattcag
    15901 ggtaactatg gcacggttac cggacgcgtg ggaatgccca gtggtgaagc tgttgcgggc
    15961 gttattgccg gtggtatggc ggaaagagga agtcagatat taacccgcca ggcagaaagt
    16021 gagccctata aacaggtaga agtgtatcag catgaagtcg tatcaattct gtttgttgac
    16081 ccggtaatga caaacgacgc caggtcctct tccctttcca gtggcatttc cccttcagtt
    16141 aatcgtacat cacaggcgga acaacgctcg caggcacgaa tgcagacagc catggagcaa
    16201 agaaaagctg tgatgcaacg tcggtatgat gaacaaccgg agacaccata atgaaacctg
    16261 aaacagaaat agatgaaacc ggcagcttcg gtgaacccga agagaaaccc gcccccttct
    16321 ggaaacgttc cgtctggggg atatctgtag caacatgggg gctatgtgca gtcgtgttgc
    16381 tcgctgccat atggtatctg ttcctgagag cgccatcgga aacaggcatg ccttcgttta
    16441 atgatgcgga tgctggcgta cagacctggc aaaccaccca agaatcatca ccttccgttc
    16501 agtccactga gactatgact gtccggacag gagaagatat gagccagctt gcccgggatg
    16561 taaaaacaga gctggataac cgggatgaaa aaattcaggc aacactgaat atgcttcacg
    16621 acagcattaa caagctgggc gaagctataa aaaaggatga agaatacgca caggaaacgc
    16681 gccgtcagct ggatgatatc cggagccgcc tgaacggaat aatgacacag aaaagcgtca
    16741 ctgaatcctc ttccacaccg catccagcaa agaaaaaaac gtcgtctgta cttaacggaa
    16801 tgaaaatcat gtcgatggaa actggcatgg cctggattcg ctggcagggt agtacctggg
    16861 ctgtcagaga aggtcagacg ctgggcaatg tggtgatcca gcggattgat ccgacgaccc
    16921 ggaccatcat caccagtgca gggacgttac gctgatgaac atggatgccc tgaccgccat
    16981 tgagaacttt gccagcagta ttttcagtgc cggaatggat ttcctgttta cctggggaga
    17041 attcatcggg gtgatcagca tgattaccct gtttgcccgc gcccgttcag ccggtccggt
    17101 aaaaatgtct cccggaaaat ttattgcggg gatgttaacc agctgcatgc tcgtatcact
    17161 cccggcaatg attaacgccg gaggcgtaca aatgggattc agggctgact catttggtcc
    17221 gattgcatat gtacaacctc agacgtttgg cgctgctgct ggcgcagcaa atgcagttct
    17281 gtcgctggcc aagctggccg gtgtgggatt tgtcatgaac gggatatcca tctggcgcaa
    17341 ggccggactc gatggacata ccgcactgag tgccagtgaa tccgtcagta aaggaaacgt
    17401 caaatttatt gccggcgtcc tgctggtctt tatcgatcgt gtcctcaatg ccctgctggc
    17461 aagcattgga atcgtcttct gatttttttc accttcatat aaaccactga tatatccatt
    17521 taaggagaaa ctatgtcacg tattcgtgtc gtggcggctc gtctgtacgc cgcatttatc
    17581 catgcacaac tgtttgtaat gtcatgccgg agcaaactga ctaagtacct gctgctcatt
    17641 cccatgatgt tgctgcccaa agccgttctt gccgatggag atcttgccga tatggttcgc
    17701 aatgttggtg aaggtgctaa aacagcacag tcatcatcaa tcactattgc gcagttcatc
    17761 ggcgtaattc tgtttctcgg gggcctgatt ggcctgaaaa aagttgggaa acaagggggg
    17821 atgggactgg ctggttgcat tgtatctatc gtcattgggg cggtacttgt cgccggacct
    17881 gaaatgatga gtcgcagcca gaaacagttg gggatttcct caatttccat tggctaatct
    17941 tgtccagggc actggcattt atccggtgcc cttcctgttc ggcaatacaa ggaggcacat
    18001 atgtctgcct ggagcccgga gcacataacg ttacgacgaa cagaaaacgg caatgtgatt
    18061 gcccacgaca gtcgggacga tcaggaattc atttttgcag aatgctgttc cgatgaaatc
    18121 gcaaacatca ttctggaagc cgtgaaacgt tacaccagta cggagcctgc tcatgcaaca
    18181 cagcattaaa gacttgtggt tgtatccctt tccagaaatt gatgtggttc atacacagga
    18241 gccactgtta ccggaaccgg aattaacaac gcccggtcga tgtatctgct gccggcaaaa
    18301 cgtcaggcac cgtttcagac tggatgactc atggccactc agacagctta ccgacacaat
    18361 cagtgacaca cgagttcgtc ttaataaggc cacagaacat ctggataagt taaaaaagcg
    18421 tggtgaacca gtcgcaaccg gcgaaaagga aaagtacaac acggcagtaa aagccgctga
    18481 acgggcgctt gagcaggccc gtctgtctgc cagacgtctt tcactccgcc atgtacagaa
    18541 agctgaaatc accagtacag agtcgttgtc agagaaagag caggaactgt ttcatgaaga
    18601 cggcccgcct tacagcttat gtgctttctg ccacgcctgg cattcactga atggttatgc
    18661 cgcagcacag ggcgttatgg tgtggctacc cgaccttcat ccatccacgg tcgtggccct
    18721 taaccgtcgt tcattacagg aagttttcag taatgataag ttccgggtcc ggcggggacg
    18781 ggaagcgctc tctgcactga tgcaaaacag actggcggtt gaggataaat tccgcagttt
    18841 ccgcccggcc gatttcgctg acgtttttcg tcgctatcca ccttccggtc gttcgcccct
    18901 gcgtgaaaaa atgaacggga ttgccctgat tctgacacca gattcattca tcaaaaaaga
    18961 atacgttgat tgatttttct tcatgccact cctgaacagg gtggcattaa aaaaatcaaa
    19021 ctgcacaaac ctctctcctg taagtccgga gttccctcac ctatgaacat aaaccgtttc
    19081 atcttcacta tcgaagactg tctgaacacg ctatcccgtt tctctgtcgc aagcagtttt
    19141 gttgagtatt gcgatttacg tactgtaatt ggtctggaca gacaggatcg tgaacgtcgc
    19201 ccatggctta attcgcccta tattgcagcc acaaagcgtg gagaatatct ctctgtcttt
    19261 gaagtcagcg gtgctttcag ggaaatggat gaagcatccg accagactgg ccctggttca
    19321 ctggaatcgc tgatcaccag catgagtgac agcctgaata cggcatataa aaacagcggt
    19381 cataaaatca gctgcgtttt cgagcgtgac cctgaaatgg ggaaagaaga aatcgaagac
    19441 atggtcgccc cacaaaaacg ctcactggct aatacaggta tccagttaca ggatgtggtc
    19501 gatgagaaag tcacaacact gtccccatgg ctggtccggg agcgttgctg gctggcgatc
    19561 tggagtggac ccgaccttat cagcaacagc gatcgcaccg cccatgatga gcttgtcaga
    19621 agactggctg aacgggtacc gaaagcacgc tttgcccagt ccccctggca gtggacctta
    19681 tctgcactga aaatccgcca cgaagctttt cttgataatg tggaacaggc actgagacat
    19741 tcatctgatg gcctgattct ccggctactg gatatacacg aggtcggacg tgaaatacgt
    19801 cgtcagacag aacggcacag cacaccgcgt aactggcaac cccacctgcc tgaagatgcc
    19861 cagccagcgg gatatcgctg gactgacgat gaatccgtac tacatgctcc ttcattgcat
    19921 ttgcagttat tcaacacgca ggtgaccaca cagggcaacc tggttcaggc gggcggactc
    19981 tggcacggaa tggtatcgat tactttacct ccgcaaaatc tgcagacgtt taacgaactg
    20041 gtccgtgctg tcccccgcgc agtcccctgg cgcatcagaa tggatctgat gcccggcggg
    20101 atgaaagctc ttaatctgaa gaaaacgctt ctgacttaca gcagcttcat ttctgccgtt
    20161 cgtccaatgt atgaatccgt tatgaccctg gcggcaacgg atgaaaaaga acctgtctgc
    20221 ataatgacca tcatggcctc cacgtgggga aaaacacgtg aaatatgcac acgaaaccag
    20281 gccattctga aatcggccat tgaaggctgg ggcgtatgtg gcaccacgac aacatttggt
    20341 gatccccgac gcgcctgggt gaataccatt ctcgcggcat ccggaggctc cggcccggtt
    20401 cccctgtatc ccccgttgtc acatgccatc tcgcttttcc cacttaacag ggcaggctct
    20461 gtctggcgag gcaaagggaa tctgatgtta catacagaag acggttccgc atttgaggtt
    20521 ggtctggcgt catcccaaca aaataaacat acagaactgg caccaggcga tccgggactg
    20581 ggtaaatcag ttctgattaa tacgttgtcg gaaatacaga tatcatctgc tcagaaaaac
    20641 ctgcccttca ttgcctatat cgataaagga tacagtgctc agggactggt tcagcttatt
    20701 cgcgacagtc ttcctccgga aagaaaagac gaagctgtcg gtattattct gagcaatgat
    20761 ccggaataca cccgtaacct ttttgacgtg atgtacggcg ctaaaaagcc tattacgccg
    20821 gaaaagaatt tcatgtcatc ggtgctgtgt gctctctgtg tcgataccgg taccggtcaa
    20881 ccctgtaacc ccggagacac ccgacaaatt atcaaccagc ttatcgagct ggcttttaaa
    20941 gagtacggtg aaaataatcc gcgactttat cgcgcctcaa cggaagacct ggtggattca
    21001 gcactgcagg actcgggact gtatgaaaaa catgatgcag cctggtgggc cagatcaacc
    21061 tggttcgaag tccgggatat gctccacaat gccgggtata tcatggcggc tcagcgggca
    21121 cattatcagg caatgcccca gcttcccgaa gtatcctcta tgctgggcca taccagtctc
    21181 agggatgttt tcggtactgt tcagcgtgat ggttcaaacg aacttctgct ggactacatc
    21241 cgccgcgccc tggagcaggg acataacgac taccccatga tatccgggta tacccggttt
    21301 atgatcaacc cggaaacccg ggtcatcgct gtcgatctga ataatgttgc cggtgacaaa
    21361 acgcctgcag gcaggcttaa aaccggcatc atgtacctgc ttgcaggtca gatcgccggt
    21421 ggtgacttca ccctgccaca atacagggat gaagtgttaa agcagcttcc acgggaatac
    21481 catgaaatcg ctcttaaacg tattaaccaa cttgatcagg aggttaagac aaaagtttat
    21541 gatgaactgc ataacgcccg gggcattgac ttcatctggg aaaatctgga tacccaggag
    21601 cgtgagcaac gcaagtttgc tatccgcacg gttctgagta ctcagtatct gagggactac
    21661 ccggaaagtg ttcttaagtc agcaaatact ctctggttac tgcgctacaa acccgaagat
    21721 atccccgtac tgagggataa cttcaacgtg cctgaattta tgctgaaacg cttcctgaaa
    21781 atgccggaag gcccggcacc ggatggtagt ggtgttcctg ttctgggagt attcagggta
    21841 aaaagtggca cgcttgcgag aatactcaaa tttaccgtcg gtccactgga gctatgggcg
    21901 ctgaactcat caccaaaaga cagtgccctg cgtaaaacgc tgacaaataa gttaggttca
    21961 gttcgggcac gtaagattct ggccgaaaac ttccccagag gttctgcaac gtcattaatt
    22021 gagcaccggg cgggccaaca taacagtgac aatgttattg aagatctggc ctcggagctc
    22081 atccgaaaac agggatacaa tctctgatgt gtacaattca tatcacccca cgctgccgga
    22141 gacctgccag agggcaggta tttcttaaaa agcttgaggt aaaaggtgac agtttctcat
    22201 tcactctgcc gtcacgtctc ttcaggttac atccggcccc caatcccaga gtgatatacg
    22261 cttcggatgc cagaacacag ggaaaggtta caggcatgcg gctgttgttg ccgtccagca
    22321 cagatgagct gataacaacg gcaatatggc aggtttctgt cccggatgaa gcaccattcg
    22381 acgttcagca tgtcatccag tggatactcc cttcaccgct ggcttcgctg atgtccgatg
    22441 atacctggtt atggccatcc gttcccggaa cgccggcact gagtgccggt gactggcctg
    22501 ttatggatac tgacccttcc accttacggc gcgtcagacg tcgttctttc agcatcatcg
    22561 ataaccggaa caggaaaggt tatatcacca ggcgatatca gtgtttccca ctggccccac
    22621 tacctgaaga cagaaaatac gacagccttc ctgaccttgt gctccatccc gagcaggaga
    22681 caataaaatg caaagaaaaa cattactcgc agcccttata accaccctga gtggaacggc
    22741 atgccaggct catgcttatt cagtcaccgt cgtcgccagc cggcctgttg aagagcaggt
    22801 catccccaga atggaagcca tcaaagatgt ccttggcaac atactgagta cccagacagc
    22861 cacgggtacc gctatcaatc agaactctga aaagctggca agtgtcattg cccagaacgg
    22921 acaggcgaca cgtcagcaga tgattttcag taatgaaact cagcgcctcg aggaggcacg
    22981 gaaaagcttc accgtgccag actccatctg cagtgaatcc gcatcaggga ttgcaacaga
    23041 aagcaaaagt gcctcagcat ccgctgcctc aaaactcagt aaaggtggcg gtgtgagcaa
    23101 ccgtagcatc cgcgaccgtc tggccagcgc agcaaactct cccgtgcggg aagcttatga
    23161 tggcgcggcc atacacgcca gttactgtac agaagcagaa tatgcacgtt ttggaggaac
    23221 cgccgtttgt ccttctgtgg gcgaaatacc aggtggagac agtcaggtac gctcgattta
    23281 ccacggcgca ggtacagcgg acacacctgc agcactgaca tgggatcaga agcaaataga
    23341 tgcagcgacg gcttacatga aaaacacttc ccgcccctct gccggtcgtg ctctgggtaa
    23401 aggagaagtg aatacccaga gtgggcgaac ctacgtcgga ctccagaacg aatacaacgg
    23461 catcattgat tctgcgtcga atccccaact gacgctgatt gctgacagca cgcctgatga
    23521 aagcacacgt aaagcactgg ctgaaacact gcagtcagac tcagcagccg cttacttcga
    23581 ccaggtggct tcaccagaag caaaagcacg gggatatatg agtacccgtg agtttgaagc
    23641 ttttgaggct ggccggcgct acgccaacac agcatacctg gtggatttac aggaaatgca
    23701 gggagataac ctgctccgtg aactggtaag aatcactgcg caaatgaact ggcagctgaa
    23761 cgacctgaaa gaacagatcc gccagggtaa tgtcatctct ggacagcaac tggctctgac
    23821 cgcccgccag tattatgaaa aacaactcgg tagccttgag aaaacaatca atcaggcaaa
    23881 cgcacgttga aagctgacgt cagaacagga ctgaaaaaat gaataagtta ccggaaaatt
    23941 cagaccagac gaaaaacgtc aaaaaatcac catccatcac ccgcatagcc acccggggga
    24001 ttattaacat tttggttcct ctgatggaga ctcgcctgat aacgaattct gtacagcata
    24061 tgctgaagca acaaaaaagc aggctggaaa aattccgtca ggtaaataat aaaaaggcgc
    24121 aactctgcct ctcgtgggaa gaagctctgc tggccagcca tatgtcagta gacgatctgg
    24181 atcgccgttt ccgtcggcgc agaacagcat ggcggttatg ctgctggagt ctgctggcca
    24241 tcgctttatt cctcagcggt atgctctttg ccgcctcctc attacctttg atgacactgg
    24301 tacgcgccat cagcactctt atgttgatac tgtcaggcgt cgctctttgt gcttccagag
    24361 ctctgatagt tacataccgc ctctggcagt tgcatgaacg aaaagtcagc gaacccgagc
    24421 aggggacttt ccgtgatttt ctgaacgacc gcaatggctg gcgcaatgcc acattaattg
    24481 ccgtaaccag taaacaatac tgatcacgtc cagatgccag taccactggc atgtcgccat
    24541 cccccttaat taaatcctgt gtggcctttg cccatacgca attctattcc ggagttactg
    24601 tgaaaatttt acttcgcgca ctctgtgcag gccttgctat atcctcactg ccggcaatgg
    24661 cttcagtgac ctatcaggat attgtgtctg ccgcaaccaa tcctgacgat ctgtcccgtc
    24721 aggcgctggt cacgatattc ggtgatgtag tcaccaatcc actgtcaacc agcgccccca
    24781 ctctgatcgg aagcatgttc ggggcgttca acagtatcat cgccgtactt gccgttgtct
    24841 ggtttatgtt cattggtatc cgtcatgttg tgcgttcggg gcatcaggga caggtattca
    24901 gcaccggacg cgatatcgtc ggcacactga gtgtcgttgc aggttttctg atgatcgttc
    24961 caaccggaaa tggctggagt atcgctcagc tcattatgtt atggggcgca tcgataatgg
    25021 gggttgggtc cgccaatgtg atggtgcaac ttgccgctga taatatcgca aatggttatt
    25081 caatgactgt acagccagtt caggcatcca cacgaacagc agcgcgtggc atttttgaaa
    25141 tggaactgtg taagtatgcg gttaacgcag gactaaatga ttttaatcag acggtcaaat
    25201 cctcgacttc actgatgaca gaatcagcta aaaccgccag tggtaattac actgtcacgg
    25261 tatccaatgg cagcggtatc tgtggttcag ccagcctgtc agtagaagga aacggaacaa
    25321 cagatcaatc gacgattgga aaattcttta atcccttcag taaaaacgaa tacagcggtg
    25381 ttatctcagc acaacgtgca gccatggata atatgattag cgacatggac aacgctgcca
    25441 gtgagtttgt gacaacattt ctggaaaaaa gaaattctgg caacggaaca ctgccggata
    25501 tcgagacacg aattcagaaa gcagccgacg aatacgaacg tgctgtgcaa aaatcgctac
    25561 ccactgataa cggagagcaa tccagaaaag aggctcttaa atcttatctc acaacctacg
    25621 ggtgggtaac gcttggcgca tggtatcaga cctttgccac tgctaaccag cgtctggcag
    25681 agctggctga cagagctcca gctgtaactt caatgtcatc actgggagaa gttggtgata
    25741 ctgacttatt cagtgcggtc atgagtgcct ataggacgca actgcagaat tcctcctata
    25801 cgccaccgct gggcacagta gtatcatcca gtgaacaacg catggcaaat gcacaagatc
    25861 cgaaatctgc cttgtctgaa attatgggcc cccttgttag tctcactaac aggattgcaa
    25921 cggaaacaag tggcacaggc acaaccagtg ctcaaattaa tcctctgata aaaatgaaaa
    25981 atatcggaga tataacaatg gtaagtgcag aaggaatctg gactatatat acaacggcca
    26041 gagttgtcgt tgccggtggc aaagattcaa ttcttgtaaa attctttaac tcactaacag
    26101 gtgccgcttc gatgcttacc gcgctgtttg aagcacttgc accacctgtt tattttcttt
    26161 tgttcctgat gttctgtgct ggattttcct tatcaattta cctgccattc attccgttta
    26221 ttttctggat gacaggcatc gggaactgga tagtcagcgt attaatagga tgtaccgccg
    26281 ggccgctgtg ggctgcaaca catctcggga catcagaaga cagaggaagt cgggcagcct
    26341 acggttatat atacttaatt gatagtatga taaggccgcc catcatggtc tttggttttt
    26401 tctttgcttc tgtagccatc atcgccgtag gcacaatact gaatgcatta tttggcgctg
    26461 ccctggttaa cgtacagttc aattcgctga ccggtatttt cagtcttgct ggtttcttgc
    26521 tgatatacgc cagaatttgt acaaccactg tagctgccat atttgccctt caggcctacc
    26581 tacctgatca tgttattaac ttcctagggg gacgtgatgg agccaacacg cttggcagca
    26641 tggcaaattc tgttaaagaa atatttattg gaggaagtcg caatatccga catactcctg
    26701 gaatgaaaac agatcgtctt aaagataata caaaaggaag tgacgacaag gacggaataa
    26761 aaggataata tcaatataaa tattgcacaa cctccccatc ggcattaaaa taatagatag
    26821 tacgagggga ggcattatat gaaagctaaa agagaaacaa cagacagatt tccaacctgg
    26881 tggctatttt attatgtatt aagaaaagct tatttctttc ttggaatccc tttttttctg
    26941 ttctgcgcat taggctttac cgaaatgcta tgttctgatc gttatttcgg aaataaggtc
    27001 gaagattatg ttgtcacttt tggtagttgg ttcttattac ttgcgccagg catatggatg
    27061 tactcacgtg caaaaacaag gagagaaaaa ataagaaaag ttgtacaaac aataaaagag
    27121 tcaggcttct actctccaga gaaggggtat gaaggattat ctttaactca gggagcttat
    27181 ttcggtattg accttaaaaa tggcacaatg ttgtatgtcc gtatttatcc aggcaacatt
    27241 atggatgtta tcggttttga cattcataat ttcactcgta ctgtaacaga cgacaaaaca
    27301 ctggaaattc atacgaaata catcaacctg ccaatggttc ctattccatc atggtgtact
    27361 catccagaaa ccgcatcaaa caccatgcac gcgatggcaa gtcgaggata tgattaccct
    27421 gtcgactttc ctcgcctgat tcaggaaaag cgtaaggagt gggagcagat tgcaggcata
    27481 cctgtagccg aagtatttta agataagctc cctccataac agccggttca aatgagccgg
    27541 cttttgttca ttacaacaga taacttatgc cgttaccgat aaattcgacc aagtgcttta
    27601 atcgcagcaa tttttatttc catatcatca ctttgactca atctatgtaa ttcacgagta
    27661 atgttctccg cctgacaccg gccttcacct aatgcataga tcgcagcaag tgtaacgtct
    27721 tttgatttac tcttcatcat ttcaaacaag aaagtattag ctatcgcagg aggcatgctc
    27781 ataatcaatc ctcttcatga aataaacaat ggcactcact tacaaacagg agtccactat
    27841 cgcaagcgac tcaacttagc aatatctctc aatttgaatg aaaaaaccag atggcatgtg
    27901 acagggaacc gtccgcatga tgtccgtgac catttaaccc cataagctac ccttgtcaca
    27961 aacgggcgca gaacagcctg ccagacagac aacaaaacaa aagtccaaga taaaacatga
    28021 ataccggtat ctcaatgcga agaaataatc tccgtagccc cccccatgag aaaaatatca
    28081 tatgggagaa aatcgcaatt atcacagcca gcgcattcat tattttcgct gtgctctggc
    28141 attaattatt ttcaacacaa aaaggaaaaa acaatgaatc tggtagacgc ctttgtcaaa
    28201 aaagtaatat ccgggccata tgaagaatat ggaaaatggt ggatagatgt cgaatacatt
    28261 tcatggggtg ttcccgggaa aacaagactg atgtttgagt caaaggagca ggcactggaa
    28321 gttaaagaag ggtacaaatt tttaacgtga gatctctttt tgttaaaggg caggaagcgg
    28381 taactccctg cccttagtta cgtttccagg cttatcagtc gataagttcc cggatcagtt
    28441 ccagaacagc aatcagcgcg tttgcaacgg caactactgc cctgagaacg atgattaaca
    28501 gattaatcat ccgcttctcc atcactggag aaagaacccc gttgttcgct cttacactgc
    28561 ctgtcacaac ggtttctata gtgtcagtta tagacagttc ttctccgacc agagcaccaa
    28621 aaccagcacc acctgatgtt ttggccagga cttgcaacct gttcttcccg ctgaacaata
    28681 tgtcggacag aacatgcctc agagctgaca tgccgagtat acacttatcc agtacttgca
    28741 gaaatataat tccagacgta ctatctacaa gtcagttata gacagttctt ttccgaccag
    28801 agccacccac tctgtaacgg aacagtaaaa aaaccgacct caccagtcgg tttttttaca
    28861 tctgttgttt ctcaaaatcc gcccgacatg cccgtcacac cggggaaact catgctttga
    28921 atcagaacca atcatctcaa ctttgccgtc tcctgaaaaa actggcacat tcagttcgat
    28981 aactacccat aaatacagag agaaaatccg atgtcagaaa agtggagtaa agaattcgac
    29041 tcttgctttc aggtggggag catagaagaa attgccgatg ctctcgagaa acgcagcgga
    29101 agcgaggaaa acgcgttagc catgctggca atgacagcct tcacactcat gacccgacgt
    29161 ggtatctgcg aaatgcagaa cgtggccccc gatggcaaag gcattcggct ggagctaatc
    29221 ggcttcaggg aaaatgaaac aataccagat acgcttcatt gaagaagaaa gggcgcaatg
    29281 aggtcagcct tcgcaacaaa gcggcggcag aaaacctgcc gccgtgttgc ccgttaacgt
    29341 ttaacttcgt aggctaacgt tgccacaagc actgtgtttc cctgctggag ccggagtccg
    29401 caaagcgaat ccctcaccat ccagacaaaa cacagaatcg tcacacagac gacgattaac
    29461 atcattaaaa acgttcgctg tggcatcata gcccctccat tgccttccgg cggttatcgg
    29521 gctactataa tgttgttgag gcattacagt agagcccttg gttgattgat aaaaaaatct
    29581 ccaggggctt tctgctgtat accgtccaca aaacgctttc ggcatacagc ctcaagcgcc
    29641 cgcagctatt ctaacgactt tcgtatctgt aacaatcctt ttattatttg aatgattcag
    29701 cgttgttttt gaagttaaca accaacccaa tacatggttc tctacttcac aacaacgcat
    29761 gttatgctga ttttagttaa taaaagaatc cccttaggag atggaaatgt cgcagttaaa
    29821 acaacctatt ttcctgaaaa agataaaaaa agtcatcaac acaatccctg ggcttgaaga
    29881 gcaaatcttt gcatgccgaa ataaaaaacg cagcgacaat ccattattat ttatcgacag
    29941 aaaagatgaa gaacgcatac taatgagtcg tctccagagc caacaaaaaa atgaagaact
    30001 ggcttctaaa ctagaatctt tatttcatgg aaacgaatta tcgtcaccac acagtatttt
    30061 gtgttttata tactggcgct atacaaagaa aatttatcgc ctaagcgagg acatcatcag
    30121 tgatgtggct aacacctatg ttgataacat tcctgcacag atcctcaagg aactcccttc
    30181 atggagtatc tatgtttcag ccgaaaatct gcatactata cttcctacgt cctatccgat
    30241 acatggattt ttcttttatc catttttgga caataatgga aatattatcc ggcttttcat
    30301 tattgatgat ctgaaacaaa gtcaaggaac tacagggcta aaagaaaagg atgttgacgt
    30361 cgtcaataat atcatcagaa taaaggactc tcgtgaagga cttcttgact ccagaaagat
    30421 ggagtgtatc gatggtgaag tcgtcgtaac agttaacgaa aagctcaaag atttcagaga
    30481 cagggagttt aatcttctta acgcacagat atcaatggtg ctttatatct gttcacagat
    30541 caatgatata aaagaaaaaa atcaatttaa acggtcagaa aagcacaaaa agcatgttca
    30601 cactcaccat gaactaccag cacaaaacat tcgggagtgg gacgttggta ttcgaatggg
    30661 gcaagccata cggcaatatc gccaagcaga acccacaggg aaagagcgga caacaatagg
    30721 tagcaaacgt cctcatatac gccggggaca ctggcataca tactggacag gaagtaagaa
    30781 accagaacta gcccatgagc gtaaacctag attaatatgg ttacctccgg taccagttaa
    30841 tttagaagac gtaaacaaac taccggtggt aataacaccc attgataaat gacctgaaaa
    30901 gtatcttttc cggtaggatg tacacatatc taaattatca ggctactata atgttgtcta
    30961 ggcattacag tagagccctt ggttgattga taaaaaatct ccagggggct ttctgctgta
    31021 taccatccac aaaacgcttt cggcatacag cctcaagcgt ccgcggctat tctaacgact
    31081 ttcgtatctg caacaatcct tttatcacac ggctaattcc tctggttagc tcttatcgcc
    31141 tgcaaataaa tttttaacct ttccgtttga tttaaattcc agaaatggag actttgtcat
    31201 gtcttacaat cgacagcctg tcgcagaaga tcctatgcag atatggggcg ctgttggtgt
    31261 ccttcttatc ctgctgctat tcgtcatatg gttattcttg ccggaagtgg tttacgccag
    31321 ctgcctgatt cttcatacct tatggggact ggttgactgg ggaccatttc ataactatgc
    31381 agcaccacga tacaacctgc ttgccatgac cggtaacaac gccgccaaca taagctatag
    31441 ccagtgggtt aacgtcatgg agcagaccat aggtattttg tggatgtacc tcttacctgt
    31501 gacactctgg tgtttgtggg aatggtatca acatccggga caaagccgtt tcacccgcag
    31561 accagtagat attacccgac tgccacacat ctttgcttcg ttgtcccctg ctatcgcccc
    31621 tgtactggaa gatggtgatc cggaaaaact ctttcatggt ggtaaacgac ctgaacggcg
    31681 ggttgctctc accccggaag catttgtcga acagcatact ctcatcacca atatgcagct
    31741 cgatgttgca gctgcaagac gatgtttcat ggcccagttg ggtaaacctt taacgtcctg
    31801 gaaagacatg gcaccacatg aaaaagcgct ctttgccata tttggccttc agtatttcct
    31861 ggatgatcgg aaggcagcat taaagctcat ggatacactg aacctctcat gcagaattaa
    31921 aagcaaacgg gacagcggga aattctgtac ccctgtttat tcactggcca aatcagcttt
    31981 ccagagagtg attaaaagca acggagcaca gcaatggctg aaacaacatc gctacgtccg
    32041 cagtgggctg gtctggctgt atgcacatga cctgcggctt acgccaccca actggatttg
    32101 gctgaaaggc gtggacagaa cgctgtttta tgcattacac cgcgccaata ccacgaaagg
    32161 atttattgaa ggcgctggcg tggtggccgt tgcacgtgca gaggccgaag ccatgcgttt
    32221 tggccttccc tgccctgaac cctgtgttga tgaagcggtg gaaggcttac ggcgggatat
    32281 gctcagtctc ggtcttatct gggatgaacc acaaccagat cgcgaccgga aacgccgcat
    32341 cctgaccaac tggtccctca ccgaagacat cctgccccgt acacctgcta cagacaacga
    32401 attctgatta tccggagaac tgaaaatgac aatcgagtac tttgcaacac gcattgcaaa
    32461 acatatttcc gtgcaggcaa tctggccgga tggcaggaca gaaattattg ccgtccttcc
    32521 acaggatgcc ctgtcagggc tggtaacacg aaataatcaa ctgattcata tagcctttga
    32581 cgccatgggc acccgtaaca gagaaaccgt cctgattgat aaacaggagc ataacgctga
    32641 ccagatactt acagctattg attcctgcct gcgccttgaa tacctgatgg aacgccgctt
    32701 cagcagaaca cctttgttcc gtggcataat cgccagcgtt gtgctgttcg taatggcaac
    32761 gattggcttc tctctgttcc gctatgttga tcgtgtattc tgggatgata caacgcctga
    32821 agcagtacag acagcaggtg agccacgact gcttccccct cacctgaatc acactgtgcc
    32881 attaaatgaa ggcattcaac tcccggttcc cccgaaaaaa gacgttcagg taccggagaa
    32941 aacaatcaca agcaaaaatc cggaagccgc agctgcgcga cataatctgg ctactgttct
    33001 gaagcggaac gccgatcggg gcatgtttac catcaacctg tcatccggtc atgaacgaac
    33061 cctgtacgca tttctcgatc ctgcctgtcc taactgcaga cttctggagc cggctttaaa
    33121 acgcctggcc agcgacttca atgtcgttat ctacccggtt tccgttattg gtggagagga
    33181 atccaccgac cgtgtggcac cactgctgtg tgaaaaagat gctcagaaac gtgctgctgg
    33241 ctggcaccga ctgtattccg cagataacgg aatgatgacg ccatcagaag aaacaacgcc
    33301 tgcagatgaa acatgcctga aagcagccag agctgcgatt gatgttaaca atgtcgcctt
    33361 ccgtaagttc ggatttgccg gtaccccatg ggtgctgtcc gacacgggct ggcatctgcc
    33421 tactggaatt ctgcaggaaa ctggcacgct taatttattc ctgaaaacca ccgacagcga
    33481 gtctggccat gagtgaacat cgcgttaacc ccgagctttt acaccgtaca gcctggggaa
    33541 atcctgtctg gaatgcactc cagagtctca atatctatgg cttttgcctg gtggcaagtc
    33601 ttgtggccag ttttatctgg ccacttgcat taccggcatg tctgttgttc acactgataa
    33661 cgatgctggt atttagtctg cagcgatggc gctgccctct tcgtatgccc atgacgctgg
    33721 aatgtgctga cccgtcacag gacagaatga ttaaaagaag tcttttcagc ttctggccaa
    33781 ccctgttcca gtatgaggtc attctggaat caccggccag cggcatattc tatgttggtt
    33841 atcaacgcgt cagggatatt ggcagggagt tatggctgag tatggacgat ctgacccgcc
    33901 atataatgtt tttcgccacc accggtggcg gtaaaacgga aaccatcttt gcctgggcga
    33961 ttaacccact ttgctgggca agaggattca cccttgtcga tggcaaggca cagaatgata
    34021 cggcaagaac gatctggtat ctggccagac gctttggacg tgaagacgat gtggaagtca
    34081 ttaacttcat gaacggagga aaatcccgca gtgaaatcat tctgagtggt gaaaaaacgc
    34141 gtcctcagtc aaatacctgg aatcccttct gttacagcac tgaggcattc acagcggaga
    34201 caatgcagtc aatgctgccc cagaacgtcc aggggggcga gtggcagtcc cgggccattg
    34261 ccatgaataa ggcactggta tttggtacta aattctggtg tgtgcgcgaa ggcaaaacaa
    34321 tgtcgctgca gatgctccgt gaacatatga ctctcgaagg gatggccaaa ctttactgtc
    34381 gtggcctcga tgatcagtgg ccggaagaag ctatcgcacc gttacgtaac taccttcagg
    34441 acgttcccgg tttcgacctg tcgctggtca ggacaccatc agcctggacg gaagaaccca
    34501 gaaaacaaca tgcttatctc tccgggcagt tttctgaaac tttcagcaca ttcacggaag
    34561 cattcgggga tatttttgct gaagattccg gtgatatcga tatccgtgac agcattcaca
    34621 gcgacaggat tctgatggta atgatccccg ctctggatac atcagcgcat acaacgtctg
    34681 ctcttggacg aatgtttatc acccagaaaa gtatgattct cgcccgtgat ttgggctacc
    34741 gcctcgaagg aactgattcc gatgcactgg aagtcaaaaa atataagggg cgtttccctt
    34801 atctgtgttt ccttgatgaa gtgggggcat actatacgga ccgtattgct gtggaagcca
    34861 ctcaggtgcg ttcactggat tttgctctga ttttgacggc acaggaccag gaacgtatag
    34921 aaggacagac aacagccacg aatactgcaa cactgatgca gaacacgggg acaaaatttg
    34981 ccggcaggat tgtcagcgag ggcagtacgg ccagaacgct gaaaagcgcc gccggtgaag
    35041 aagccagggc gagaatgaac aacctgcagc gtcaggacgg catatttggt gaatcatgga
    35101 ttgacagccc acaaatcagc atcctgatgg aaagcaagat aaatgttcag gagttgattg
    35161 aacttcatcc tggcgaattc ttttctattt tccggggaga aaccgtacca tcagcatcat
    35221 tcttcatccc tgacgatgag aaaagctgca gcagcgatcc cgttgttata aacaggtata
    35281 tcagcgtgga tgcgccacgc ctggaccgtc tccgccgtct tgttccccgt accactcaac
    35341 gtcgaatacc gtcaccggaa aatgtcagtg ccatcattgg cgtcctgact gcaaaaccct
    35401 ccagaaaaag acgaaaaatt cgcacagagc cgcacacgat cgtggataca ttccagcaac
    35461 gcattgcagg acgtcaggcg gctatggcaa tgctggaaga gtacgacaca gatatcaacg
    35521 cacgtgaaag tgccttgtgg gaaacagcgg tgaatacgct taaaacgacc acacgggaag
    35581 agcgacgaat tcgctatatc acgctgaaca gaccagaact acctgaaaca aaagaggaaa
    35641 atcagatatc agtgagagca gaaagagcag gtataaacct gttaacgctt ccacaggaca
    35701 ataaccaccc gacagggcgt cctgtaaacg ggtttcatca taaaaaaa
//
LOCUS       DAOTRK010000034        35596 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.034, whole genome shotgun sequence.
ACCESSION   DAOTRK010000034 DAOTRK010000000
VERSION     DAOTRK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.034"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..496)
                     /locus_tag="QIE41_001655"
     CDS             complement(<1..496)
                     /locus_tag="QIE41_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HDS3530550.1"
                     /translation="MAISSYSTVTDKFATLTKMQDENLNEKNRTQKYSGKIGLSVDDV
                     GKYSVTHKDITNILAGDSQSFLKRSLWEFILDLFPMYHIKEAKQAIIDFVSETGDKAD
                     IFNKIKSLADQDQQWRFNATTEFIVDENKDIIVSRVFKININAQDHHIDAEKKTMHSL
                     FSEKI"
     gene            complement(923..1648)
                     /gene="phoU"
                     /locus_tag="QIE41_001656"
     CDS             complement(923..1648)
                     /gene="phoU"
                     /locus_tag="QIE41_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDS3530551.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            complement(1663..2436)
                     /gene="pstB"
                     /locus_tag="QIE41_001657"
     CDS             complement(1663..2436)
                     /gene="pstB"
                     /locus_tag="QIE41_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDS3530552.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2527..3417)
                     /gene="pstA"
                     /locus_tag="QIE41_001658"
     CDS             complement(2527..3417)
                     /gene="pstA"
                     /locus_tag="QIE41_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDS3530553.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(3417..4376)
                     /gene="pstC"
                     /locus_tag="QIE41_001659"
     CDS             complement(3417..4376)
                     /gene="pstC"
                     /locus_tag="QIE41_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDS3530554.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4463..5503)
                     /gene="pstS"
                     /locus_tag="QIE41_001660"
     CDS             complement(4463..5503)
                     /gene="pstS"
                     /locus_tag="QIE41_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDS3530555.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAITKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5817..7646)
                     /gene="glmS"
                     /locus_tag="QIE41_001661"
     CDS             complement(5817..7646)
                     /gene="glmS"
                     /locus_tag="QIE41_001661"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDS3530556.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(7808..9178)
                     /gene="glmU"
                     /locus_tag="QIE41_001662"
     CDS             complement(7808..9178)
                     /gene="glmU"
                     /locus_tag="QIE41_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDS3530557.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQADKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(9531..9950)
                     /gene="atpC"
                     /locus_tag="QIE41_001663"
     CDS             complement(9531..9950)
                     /gene="atpC"
                     /locus_tag="QIE41_001663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDS3530558.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9971..11353)
                     /gene="atpD"
                     /locus_tag="QIE41_001664"
     CDS             complement(9971..11353)
                     /gene="atpD"
                     /locus_tag="QIE41_001664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDS3530559.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(11380..12243)
                     /gene="atpG"
                     /locus_tag="QIE41_001665"
     CDS             complement(11380..12243)
                     /gene="atpG"
                     /locus_tag="QIE41_001665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDS3530560.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(12294..13835)
                     /gene="atpA"
                     /locus_tag="QIE41_001666"
     CDS             complement(12294..13835)
                     /gene="atpA"
                     /locus_tag="QIE41_001666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDS3530561.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(13848..14381)
                     /gene="atpH"
                     /locus_tag="QIE41_001667"
     CDS             complement(13848..14381)
                     /gene="atpH"
                     /locus_tag="QIE41_001667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDS3530562.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(14396..14866)
                     /gene="atpF"
                     /locus_tag="QIE41_001668"
     CDS             complement(14396..14866)
                     /gene="atpF"
                     /locus_tag="QIE41_001668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDS3530563.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14928..15167)
                     /gene="atpE"
                     /locus_tag="QIE41_001669"
     CDS             complement(14928..15167)
                     /gene="atpE"
                     /locus_tag="QIE41_001669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDS3530564.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(15214..16029)
                     /gene="atpB"
                     /locus_tag="QIE41_001670"
     CDS             complement(15214..16029)
                     /gene="atpB"
                     /locus_tag="QIE41_001670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDS3530565.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(16038..16418)
                     /gene="atpI"
                     /locus_tag="QIE41_001671"
     CDS             complement(16038..16418)
                     /gene="atpI"
                     /locus_tag="QIE41_001671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDS3530566.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(17035..17658)
                     /gene="rsmG"
                     /locus_tag="QIE41_001672"
     CDS             complement(17035..17658)
                     /gene="rsmG"
                     /locus_tag="QIE41_001672"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDS3530567.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(17722..19611)
                     /gene="mnmG"
                     /locus_tag="QIE41_001673"
     CDS             complement(17722..19611)
                     /gene="mnmG"
                     /locus_tag="QIE41_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDS3530568.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19990..20433)
                     /gene="mioC"
                     /locus_tag="QIE41_001674"
     CDS             complement(19990..20433)
                     /gene="mioC"
                     /locus_tag="QIE41_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDS3530569.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(20523..20981)
                     /gene="asnC"
                     /locus_tag="QIE41_001675"
     CDS             complement(20523..20981)
                     /gene="asnC"
                     /locus_tag="QIE41_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDS3530570.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            21133..22125
                     /gene="asnA"
                     /locus_tag="QIE41_001676"
     CDS             21133..22125
                     /gene="asnA"
                     /locus_tag="QIE41_001676"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDS3530571.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(22130..23581)
                     /gene="viaA"
                     /locus_tag="QIE41_001677"
     CDS             complement(22130..23581)
                     /gene="viaA"
                     /locus_tag="QIE41_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDS3530572.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(23575..25071)
                     /gene="ravA"
                     /locus_tag="QIE41_001678"
     CDS             complement(23575..25071)
                     /gene="ravA"
                     /locus_tag="QIE41_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDS3530573.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            25294..27162
                     /gene="kup"
                     /locus_tag="QIE41_001679"
     CDS             25294..27162
                     /gene="kup"
                     /locus_tag="QIE41_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDS3530574.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            27329..27748
                     /gene="rbsD"
                     /locus_tag="QIE41_001680"
     CDS             27329..27748
                     /gene="rbsD"
                     /locus_tag="QIE41_001680"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDS3530575.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            27756..29261
                     /gene="rbsA"
                     /locus_tag="QIE41_001681"
     CDS             27756..29261
                     /gene="rbsA"
                     /locus_tag="QIE41_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDS3530576.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFAREQATQEVLMAAAVGKLNRVNQE"
     gene            29266..30231
                     /gene="rbsC"
                     /locus_tag="QIE41_001682"
     CDS             29266..30231
                     /gene="rbsC"
                     /locus_tag="QIE41_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDS3530577.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            30256..31146
                     /gene="rbsB"
                     /locus_tag="QIE41_001683"
     CDS             30256..31146
                     /gene="rbsB"
                     /locus_tag="QIE41_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDS3530578.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            31272..32201
                     /gene="rbsK"
                     /locus_tag="QIE41_001684"
     CDS             31272..32201
                     /gene="rbsK"
                     /locus_tag="QIE41_001684"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDS3530579.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWVSVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            32205..33197
                     /gene="rbsR"
                     /locus_tag="QIE41_001685"
     CDS             32205..33197
                     /gene="rbsR"
                     /locus_tag="QIE41_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDS3530580.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(33163..34590)
                     /gene="mdtD"
                     /locus_tag="QIE41_001686"
     CDS             complement(33163..34590)
                     /gene="mdtD"
                     /locus_tag="QIE41_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDS3530581.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(34613..35305)
                     /locus_tag="QIE41_001687"
     CDS             complement(34613..35305)
                     /locus_tag="QIE41_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDS3530582.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         8652 a         9126 c         9009 g         8809 t
ORIGIN      
        1 tgattttttc tgaaaataaa gaatgcattg tctttttttc agcatcaata tgatgatctt
       61 gcgcattaat atttatttta aatactctac taacaataat atctttattt tcatctacaa
      121 taaattctgt cgtcgcatta aagcgccatt gttgatcttg atcggcgaga gatttaattt
      181 tattaaatat atcagcttta tcaccagttt ccgacacgaa atctataatg gcttgtttag
      241 cctccttaat atgatacatg ggaaaaagat cgagtataaa ttcccaaaga cttcgtttaa
      301 ggaaagattg actatcacca gctaaaatgt ttgttatatc tttatgagtt actgaatact
      361 tacccacatc atcaacagac aaaccaattt ttcctgaata tttttgcgtt ctattttttt
      421 cattcagatt ttcatcttgc attttagtca gagtagcaaa tttatctgta actgtactat
      481 atgatgatat agccataatc atttctcctg aacacaattt ttattcgccc gctcacaata
      541 cataaccgtt ttgtataaaa aagaacactt tggtgttgtt ttgatacgat tatgactaaa
      601 ccacctatga atactgtaaa aaatcgctca ttttttatgt tccgatatag ggcttttacc
      661 ctggtttttt gttttctttt ccattttctg tgcgggtaac gatataaatg aaaggaataa
      721 caaaaaaaat tttataacgg ggcactacat taaaagtaaa ataattcaaa tgaaataata
      781 tatttgcatt gcactgggga gagtttcgag gtgagtttgt gtggcaatat ttcagggaga
      841 aagtgtatca gcccggtaac tatgtatgac cgggctgatt gccagattcg gcgtaaaagc
      901 cttatctgac ctacagttag cgttactctt tcgggtcttt tccggccagc agtttatcca
      961 gctcatcacc gcctacatga cggaaatcct gccccttcac gtagtagaag ataaactcac
     1021 aaatattctg gcagcggtcg ccgatacgtt cgattgaacg cgcacagaat agcgcggtaa
     1081 gtacgctcgg aatggtgcgc gaatcttcca tcatgtaggt catcagttga cgaacaatac
     1141 cttcgtactc ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt
     1201 ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg
     1261 actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc
     1321 agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca
     1381 taaccagacg caggtcgctc gccgtcggct gacgtttggc gataatgcgc acgcaggctt
     1441 catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatgacg cgcttcgcca
     1501 gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc
     1561 cgcccatggt catcacctgc gtgcggatgc tttccagttc ggcgttgaac tggccggaaa
     1621 tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt
     1681 gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa
     1741 ctcaatcaat tcgcccaggt acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg
     1801 catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc
     1861 aatacgcccg gtagagatgg ggtcgagtgc cgaacacggt tcgtcgagca gcagcacttc
     1921 cgggcgaatg gcgataccac gcgcaatgca cagacgctgt tgctgaccac cagagagaga
     1981 gtaaccgctc tgatgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc
     2041 ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gcacaccaaa
     2101 ggcgatgttg tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca ccatgcccac
     2161 tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcgagcag
     2221 aatttcacct tccgcacgct gttccgggta cagttcaaac attttattga aggtacgcag
     2281 cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gttacctggt ttttagcgat
     2341 atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac
     2401 ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct catcgtgcct
     2461 gatgcacttc gctaatcagg cttaccgtag gccggatcag gcgttcacgc cgcatccggc
     2521 aaagtgttaa ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag
     2581 gacgcacagg gtaatgatca ataccccggc ccaggccagt tgctgccatt ccgcaaacgg
     2641 actcatcgca aacttaaaga tcgtcaccgg caggttggcg atcggctgca tcatgtccgt
     2701 gctccagaac tggttggaga gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg
     2761 ggcaatcgcc agcaggatac cggtcataat cccggacacc gacgctttca gcgtaatcgc
     2821 ggagatcatc ttccacttcg gcgtacccag cgcataagcc gcttcacgca ggctgtccgg
     2881 caccagtttc agcatgttct cggtggtgcg gataacaatc ggcacctgca acagcgccag
     2941 cgcaatcacg cccgcccagc cggagaagtg ctccatctgc gccaccacga tggtgtaaac
     3001 aaacagacca actacaatcg acggtgcgga gagcagaatg tcgttaataa agcgaatcac
     3061 ttctgccagc caggatttac gaccatattc cgccagataa atccccgcca taatgcccag
     3121 cggcgtaccg aataccgtgg cccacaaaat taacagcccg ctacccgcca gggcgttcgc
     3181 cagaccaccg ccttccgtat tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat
     3241 accatcgata ccgcgagtga tggtggacat taaaatccag atcagccaga acaggccgaa
     3301 ggccatcgtc gccattgaga gcgtcagcgc aatacggttt ttgaggcgac gacgcgcctg
     3361 cattttgcgg cgagattcag ccagcgccgc agtggtttgc atttcaacca tagccattag
     3421 cgtgccccct cattcttagc caggcgcata atcataaact tcgatgcggc gaggacgatg
     3481 aaggtaatca caaacaggat caggcccagt tccatcagtg cggcaacgtg cagaccggat
     3541 tccgcttcgg caaattcgtt cgccagcgca gaggtgatac tgttgcccgg catgtacagc
     3601 gaggcgctgt cgagctgata ggtgttaccg atgataaagg tcaccgccat ggtttcaccg
     3661 agcgcacgcc ccagccccag catgatgccg ccgataaccc catttttggt gaacggaaga
     3721 acgatacgcc agataacttc ccaggtggtg cagccaatac cgtaggccga ctctttcatc
     3781 atcaccgggg tttgttcgaa cacatcgcgc attaccgccg caatgtacgg aataatcatg
     3841 atggcgagga tcacgcctgc cgcaaggata ccgataccaa atgcggggcc agagaacagc
     3901 gcgccgacaa tcgggatgtt cgacatgata ttgccgaccg gctcctggaa gtaaacggcg
     3961 aacagcggcg caaagataaa caggccccac atgccgtaaa cgatacttgg aatggcagcc
     4021 agcagctcaa tggcgatacc cagcgggcgt ttcagccagc caggcgcaag ctcggtcagg
     4081 aacagggcga taccaaaact caccggaacg gcaatcagca gcgcgataaa cgaggtcacc
     4141 agcgtaccgt agatcggcac cagcgccccg tagatatcgt tcggcgcatc ccactctttg
     4201 gtccacagga aagccagacc aaatttctga atgctcggcc aggaggagat gatcagagag
     4261 acaataatgc cacccaacat caatagcaca atcagcgccg ccagttttac tagcacgctg
     4321 aaaattatgt cgcccttttt acccggtggg ttaaaagcag gcttggttgc agccataagt
     4381 tactcttcag ttaaacgcgt ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca
     4441 ccgtacccgg cctggagttt tattagtaca gcggcttgcc gctactgtct ttaatattgg
     4501 tcttccacgc agcgcgaacc tgctcaacta cgctatccgg caggctggca taatccaggt
     4561 cgttcgcctg tttagccccg gttttgtatg cccagtcgaa gaacttcagc acttcggtgc
     4621 cttgttctgg tttcttctga tctttatgga tcagaatgaa cgtggtagag gtaataggcc
     4681 atgcatcttc gcctttctgg ttggtcagat cctgagcgaa tgttttgctc cagtctgcgc
     4741 cttttgcagc gttagcgaag ttttcttcgg tcggcaaaac aggtttacca tcagcggaga
     4801 tcagtttggt gtacgccagg ttgttctgct tcgcgtaagc atattcaacg taaccaattg
     4861 cacctggcag acgctgaacg aacgcggcga taccgtcgtt acctttaccg cccagaccaa
     4921 tcggccattt tacggtagag cccgtaccaa cgttgttttt ccactcttcg ttcactttcg
     4981 ccaggtagct ggtgaagacg aaggacgtcc cggagccatc tgcgcggcgt actacagcaa
     5041 tgttctgtga cggcagtttt aagcctggat tcagtttggt gatggcttca tcatcccact
     5101 tcttgatttt gccgaggtag atgtcaccaa gggttttacc atccagcacc agctcgccag
     5161 acttcaaccc tggaatgtta accgccagta ccacgccgcc aatcacggtc gggaactgga
     5221 acaaaccttc ctgagccagt ttttcgtcag acagcggcgc gtcagaggca ccgaaatcaa
     5281 cggtatttgc gataatctgt tttacgccac cggaagaacc gataccctgg tagttaactt
     5341 tattaccggt ttctttctgg taagtgtcag cccatttggc atacaccggc gcagggaagg
     5401 ttgcacctgc acctgtcagg cttgcttctg caaacacaga gaaagcactc atcgataagg
     5461 tcgcggcgac aacagttgcg acggtggtac gcataacttt cataatgtct cctgggagga
     5521 ttcataaagc attgtttgtt ggctacgaga agcaaaatag gacaaacagg tgacagttat
     5581 atgtaaggtt tatgacagtt ttatgacaga gagagaaagt ctttagtctg atttaaataa
     5641 gcgttgatat tcagttaatt acaaatatta ataacgaaga gatgacagaa aaattttcat
     5701 tctgtgacag agaaaaagta gccgaagatg acggtttgtc acatggagtt ggcaggattt
     5761 ttgattaaaa acataacagg aagaaaaatg ccccgcttac gcagggcatc catttattac
     5821 tcaaccgtaa ccgatttcgc caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc
     5881 gcaacgtggt aagccagcag ctgcagcgga acggtgtaga agatcggtgc aatcacctct
     5941 tccacatgcg gcatctcgat gatgtgcatg ttatcgctac ttacaaaacc cgcatcctga
     6001 tcggcgaaga catacaactg accgccacgc gcgcgaactt cttcaatgtt ggatttcagt
     6061 ttttccagca attcgttgtt cggtgcaacg acgataaccg gcatatcggc atcaatcagc
     6121 gccagcggac cgtgtttcag ttcgcctgca gcgtaggctt cagcgtgaat gtaagagatc
     6181 tctttcagct tcaatgcgcc ttccagcgcg attgggtact gatcgccacg gcccaggaac
     6241 agcgcgtgat gtttgtcaga gaaatcttct gccagtgctt caatgcgttt atcctgagac
     6301 agcatctgct caatacggct cggtagcgcc tgcagaccat gcacgatgtc atgttcaatg
     6361 gaggcatcca gacctttcag acgagacagc ttcgccacca gcatcaacag cacggttaac
     6421 tgggtggtga atgctttagt ggacgccacg ccgatttctg tacccgcgtt ggtcattagc
     6481 gccagatcgg attcgcgtac cagcgaagaa cccggaacgt tacagattgc cagtgaacca
     6541 aggtaaccca gctctttcga cagacgcagg ccagccaggg tatccgcggt ttcgccagac
     6601 tgtgacaagg tgatcatcag gctgttacga cgcacggcag atttgcgata gcggaattca
     6661 gaggcgattt cgacgtcgca cggaatacct gccagcgatt caaaccagta gcgggaaacc
     6721 ataccggagt tataagaagt accacaggcg aggatctgaa tatgctcaac cttcgacagc
     6781 agttcgtcgg cgttcggtcc cagctcgctt aaatcaacct gaccgtggct gatgcgcccg
     6841 gtaagggtgt ttttgatcgc gttcggctgt tcgtagatct ctttctgcat gtagtggcgg
     6901 taaatacctt tatcgcccgc gtcatattgc agattggatt cgatatcctg acgttttact
     6961 tccgcgccag ttttatcgaa gatgtttacc gaacggcgag tgatttccgc aatatcgccc
     7021 tcttcaagga agataaagcg acgagtcact ggcaacagcg ccagctggtc agaagcgata
     7081 aagttttcgc ccatacccag gccaatcacc agcggactac cagaacgtgc cgccagcagg
     7141 gtatccggat gacgggagtc catgatcact gtaccgtacg caccacgcag ttgcgggata
     7201 gcacgcagaa cagcctcacg cagagtaccg ccttgtttca actcccagtt caccagatgg
     7261 gcaatcactt cggtgtcggt ttcagaaacg aaggtatagc cacgcgcttt taactcttca
     7321 cgcagcggtt catggttttc gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca
     7381 tgcggatgcg cgttagcttc tgaaggttca ccgtgggtcg cccagcgagt atgagcaata
     7441 ccggtgccgc catgcagagg atgttcttcc gctgcctgag ccagcatctg gactttaccg
     7501 aggcgacgca ggcgggtcat atgaccttct gcatcaacaa cggccagacc ggcagagtca
     7561 tatccgcggt attcaagacg acgtaaacct tcaagaagga tttctgctac atcacgttgc
     7621 gcgatcgcgc caacaattcc acacatagtt tttgattccg atttatatcg ttgtcggtca
     7681 acctgtatgc ccgttttttc gggcaccccg agccttgtag agagtggggt tatttttata
     7741 gttactgctt gtgggcggga gattatgtta tctcctcatc ccatatgacc gtattacccg
     7801 gccagaatta ctttttcttt accggacgac gccagccttc tttttgagtc tgcggcacac
     7861 ggctgatcgc cagggcgttt tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg
     7921 tcgcgccttt gcctactgtt accggggcca ccagctgagt gtcggaaccg acaaacacat
     7981 cgtcaccgat aatggtctta aatttattcg caccatcgta gttgcaggta atggttcccg
     8041 cgccgatgtt aacgttatcg ccaatttccg catcgcccag gtaagtcaga tgaccagctt
     8101 tcgagccttt acccagacgc gcttttttca tctcaacgaa gttaccgacg tgtgcacctt
     8161 ccagcaactc agcaccagga cgcagacggg caaacgggcc aatggtacag gccgctgcca
     8221 gattcgcatc ttccacaacg gtatacgggc taatttcgca atcatcgcca atcacgctgt
     8281 ttttaatcac gcaaccggtg ccgattttca cgcgatgacc gagagtcacg ttgccctcga
     8341 tgataacgtt agtatcaatt tcaacatcgc gcccgtgagt aagcgtaccg cgcagatcaa
     8401 aacgcgccgg atcgcgcagc ataacgcctg ctaacagcag tttatcagcc tgttcggact
     8461 ggtaaacgcg ctccagtcgg gagagttgca ggcggttatt cacgccttct acttcgctta
     8521 aacgttgcgg atgaacggcg acgatttcac gcccttcctg atacgccagc gcaataatgt
     8581 cggtgatgta gtattcaccc tgagcgttat tgttggtcag cttcgccagc cagcgtttca
     8641 tatctgcgcc gttggcaatc agaatgccgg tgttgatctc ctgaatctga cgctgctcgt
     8701 cggtggcgtc tttgtgctca acaatgccgg taactttgcc gttttcacgg gtgatacgtc
     8761 cataaccggt cggatcatcc agttttaccg tcagcagacc aatgccaccc tgcggtttag
     8821 catcacgcag gcgctggagt gtttcgacag agatcagcgg cacgtcgccg tagagcatta
     8881 aaatgtcttc atcatcggca aagaaaggtg cggcctgctg catcgcatga cctgtaccca
     8941 gctgttctgc ctgaagcacc cagttcaggt tgtcatcttt cagcgcctgt ttaagcagat
     9001 cgccgccgtg accgtacacc aggtgaacgt gcgccgcgcc taattcattc gcagcatcaa
     9061 tgacatgctg aaccatcgct ttcccggcaa gggtatgcag cactttcgga agatcggaat
     9121 acatgcgcgt gcctttgcct gcggcaagga tcactacgct catagcatta ttcaacatac
     9181 gcgtcctgac tgtaatttga gaacgaattt aaaccgcttc accttgaaaa aactacattt
     9241 ttttcatcgt gaaatggaca gaggataaat tgttcaataa cggattatcc ccgctgacga
     9301 aagtgccatt ttcgaccatc gtcacgcctt ttgtctgcag aaaattaagg cagtaccttc
     9361 atttaagcgt caggtggatg tttttgctct tatttcgatc aatgaataaa cgggaaaaca
     9421 gggtgtttta tatcagcagg atctatgtga acgctcttca ggacgggtca atacgcgtaa
     9481 aaaaaagcca gcctgttacc agactggctt ttgtgctttt caagccggtg ttacatcgct
     9541 tttttggtca actcgataac gcgcagctgc gcgatcgctt tggccagttc cgcagacgcc
     9601 tgagcgtaat ctacgtcgcc gtgagagctg ctaatgtgct cttcagcctt acgtttcgct
     9661 tccatggctc gcgcttcgtc gagatcctgg ccgcgaattg cggtgtcggc cagaacggtc
     9721 acgttgccag gctgcacttc aagaatgccg ccagacagat agataaactc ttcgtgaccg
     9781 tgctgtttca cgatgcgaat cataccaggc ttaatggcgg tgagcagcgg tgcgtggcca
     9841 gggtagatcc ccagttcacc ttcgctaccc gttacctgga ttttctcgac cagaccagag
     9901 aacatttgtt gctctgcact gacgacgtcc aggtggtaag tcattgccat atcaccctcc
     9961 gattaaggcg ttaaagtttt ttggcttttt ccacagcttc ttcgatggaa ccgaccatgt
    10021 agaacgcctg ctccggcagg tgatcgtatt cgccttccat gatgccttta aagccacgga
    10081 tggtgtcttt cagggagacg tatttacccg gagaaccggt gaatacttct gccacgaaga
    10141 acggctggga caggaagcgc tggatcttac gagcacgcgc taccaccagt ttgtcttctt
    10201 cagacagttc atccataccc aggatggcga tgatgtcttt cagttcctga taacgttgca
    10261 ggatggactg aacgccacgc gcggtgtcgt agtgttcctg accaaccacc agcgggtcca
    10321 gctgacggct ggtggagtcc agcgggtcaa cggccgggta gatacccaga gacgcgatct
    10381 gacggctcag taccacggtt gcgtcaaggt gcgcaaaggt ggttgccgga gacgggtcag
    10441 tcaagtcatc cgcaggtacg tataccgcct gtacggaggt gatagaacca gttttggtgg
    10501 aggtgatacg ttcctgcaga acgcccatct cttccgccag ggtcggctga taacctaccg
    10561 ctgaaggcat acggcccagc agtgcggata cttccgtacc ggccagggtg taacgataga
    10621 tgttgtcaac gaacagcaga acgtcacgac cttcgtcacg gaatttctca gccatggtca
    10681 gaccggtcag agcaacgcgc agacggtttc ccggcggctc gttcatctgg ccatacacca
    10741 gggatacttt gtcgataacg ttggagtcgg tcatttcgtg gtagaagtcg ttaccctcac
    10801 gagtacgttc acctacgccc gcaaacacag agtaaccgga gtgctcgatc gcgatgttac
    10861 gaataagctc catcatgttt acggttttac ctacgcccgc accaccgaac agaccaactt
    10921 taccgccctt agcgaacgga cacatcaggt cgataacttt gataccggtt tccagcagtt
    10981 cctgagagtt tgacagctct tcgtaggaag gtgctgcgcg gtgaatcgcc caacgctctt
    11041 cttcaccgat ctcgcctttc atgtcgaccg gttcacccag tacgttcatg atacggccca
    11101 gagtcgcttt acctaccggg acttcgatcg ggtgttcgag gtcttttaca tccagaccgc
    11161 gacgcagacc gtcggaggaa cccattgcga tggtacgcac gataccgccg ccgagctgct
    11221 gctgaacttc cagcaccaga cgctcattac cattttgcac ctcaagagca tcgtacacgc
    11281 gcggtacggc atcctgaggg aattcgacgt caactacggc gccgattacc tggacaatct
    11341 ttccagtagc catcttaaat cctctacgaa ataacctgtt taaaccgcgg cggcccccga
    11401 gacgatctcg gtgagttcct gagtaatgct ggcctgacga gctttgttgt ataccaactg
    11461 cagctcttta atcaggctgc cgccattgtc ggtcgcggct ttcatcgcca ccatacgggc
    11521 ggcctgctcg ctggccaggt tttcaaccac gccctgataa acctgagatt cgacataacg
    11581 acgcagcagg gtatccagca acgccttcgg atcgggttcg tacaggtaat cccaggattt
    11641 gtgtttcaga tcatcatcat ctgatgccgg taacggcagc agctggctga tggtcggaac
    11701 ctgagacatg gtgttaataa atttgttgct gacaatgtaa agcttgtcca gacggccttc
    11761 gtcgtaggcc tgcaacatca cttttaccgg accgatcagt tcggacaggg aagggttatc
    11821 ccccatgcca gtgacctggg caacaacatt gccgcccacg gagttgaaga acgacacgcc
    11881 tttcgagccg atcattgcga ggtcgcattg aacgcctttg tcggtccagg tcttcatttc
    11941 cgccagcagt tttttgaaca ggttaatgtt caaaccaccg cacaaaccac ggtcggtcga
    12001 caccaccagg tagcccacgc gtttaacgtc gcggtcttcc aggtaagggt gcttatattc
    12061 cagattaccg tgtgcaaggt gaccaatcac tttgcgcatg gtttctgcat aaggacggct
    12121 ggccgccatg cgatcctgcg atttacgcat tttggaagcg gcgaccatct ccatcgcttt
    12181 agtgatcttt tgcgtgttct ggacgcttgc gatcttacta cgtatctctt ttgcgccggc
    12241 catgagcttc tcctcaatgc cttgcggcct gccctaaggc aagccgccag acgttaccag
    12301 gattgggttg ctttgaagga atcgaggatg cctttcagct tgccttcgat ttcgtcgttg
    12361 tagccaccgg tctggttgat ctcttgcatc aacggagcgt gatcacggtc gacgtaagcc
    12421 agcagagcgg cttcgaagct gccgattttc gacagttcaa catccgccag gtaaccacgt
    12481 tctgctgcga acagaaccag agactgttgc gctacggaca tcggcgcata ctgtttctgt
    12541 ttcagcagtt cggtcacttt ctgaccgtgg tcaagctgct tacgtgttgc atcgtcaagg
    12601 tcggatgcaa actgagagaa cgctgccagt tcacgatact gtgccagagc ggtacggata
    12661 ccaccggaca gttttttcat gatcttggtc tgtgctgcac caccaacacg ggatacggaa
    12721 atacccgggt taaccgcagg acgaataccg gcgttgaaca ggttggtttc caggaagatc
    12781 tgaccatcgg taatggagat tacgttggtt ggaacgaacg cagaaacgtc acccgcctga
    12841 gtttcgataa tcggcagcgc agtcagagaa ccggttttcc ctttcacttc acctttggtg
    12901 aaggcttcaa cgtattcggc gttaacacgc gcagcacgct ccagtagacg agagtggagg
    12961 tagaatacgt cgcccgggaa tgcttcacgt cctggcggac gacggagcag cagggagatc
    13021 tgacggtaag caacagcctg tttagacagg tcatcgtaaa tgatcagcgc atcttcaccg
    13081 cggtcacgga agtattcgcc cattgcgcaa ccagcgtacg gtgccaggta ttgcagtgca
    13141 gcggattcag acgcggttgc taccacaacg atggtgttag ccagtgcgcc gtgctcttcc
    13201 agtttacgta ccacgttaga aatggtggac gctttctggc cgatagcgac atagatacat
    13261 ttgataccgg aatcgcgctg gttgatgatg gcatcgatag ccaatgcggt tttaccggtc
    13321 tgacggtcac cgatgatcaa ttcacgctga ccacgaccga ttgggatcat ggagtcaacg
    13381 gctttataac cggtctgtac cggctgatct acggactgac gttcgataac gcccggagcg
    13441 attgcttcta cagcagagaa gccgtcgtga tccagcggac ctttaccgtc gattggtgca
    13501 cccagagtgt taaccacacg gcccagcagg ccacggccaa ccggaacttc caggatacgg
    13561 ccagtacact taactttcat gccttcggca aggtcagcgt acggacccat aacaaccgca
    13621 cctacagagt cgcgctcgag gttcagtgcg atagcgtaac ggttacccgg cagggagatc
    13681 atttcaccct gcatacaatc ggccaggccg tgaatgcgga taacaccgtc acttacagaa
    13741 acaatagtac cttcgttgtg agcttcactc acaacattga actgagcaat gcgctgcttg
    13801 atcagttcgc tgatttcggt ggaattcagt tgcatgctcc agtcccctta agactgcaag
    13861 acgtctgcaa ggcgctcaag acgaccgcgt acgctgccat caatgaccat atcacccgct
    13921 cggatgataa cgcctgccat tacagactta tcgattttgc aattcagctt aactttgcgt
    13981 gacagacgtt tttccatcgc agcagaaatt ttcgcgagct gttgttcact cagtgcggca
    14041 gcggaaatga cgtctacctc agcggtagcc tcactcacgg cacgcaggtg aataaactgc
    14101 tccagaacat ccgggagcgc gttaagacga ccattttcag ccattacccg aatcaggttc
    14161 tgaccgtttt cgtccagttg ctcaccacaa actgcgataa acgactcggc gagcgtttct
    14221 ggcgcaagcg cgccagagag aagctctgcc atttgttcgt ttttggttac ctcggcggca
    14281 aacgccagca tgtcctgcca gcgttctaca ctttggtgtt cgacggcaaa gtcaaaagct
    14341 gctttggcgt aggggcgagc taccgtaata aattcagaca tcagcccctc cctccttaca
    14401 gttcagcgac aagtttatcc acgatgtcgc tgttagcagc ttcatccacg gaacgttcga
    14461 tgatcttctc ggcgccagca acagccagga tagcaacttg cttacgcagc tcttcacggg
    14521 cacgtttacg ctcggcttca atttccgcct gcgcctgggc cacgatttta gtacgttcct
    14581 gttctgcctc agctttcgct tcgtccagaa tctgcgagcg gcgtttgtta gcctgctcga
    14641 tgattacctg ggcttccgct ttcgcttttt tcagctggtc ggtcgcgctg gcctttgcaa
    14701 ggtcaaggtc cttatgtgct cgttctgcgg aagcaagacc gtcagcaatt tctttttgac
    14761 gtttttcgat ggctgccatt aatggcggcc atacgtactt catgcagaac agaacgaaca
    14821 ggacaaacgc gatggcctgg ccgaggattg ttgcgttaag attcacagca caatgcctct
    14881 atttagttaa cgttctgata ttgctcttta aataaaagca acgcttacta cgcgacagcg
    14941 aacatcacgt acagacccag acctacagcg atcatcggga tagcatccac cagacccata
    15001 acgataaaga actgagtacg cagcagagga atcagatcag gttgacgcgc tgcgccttcc
    15061 aggaatttac ccccgaggat gccgataccg atcgcagcac cgattgccgc cagacccatc
    15121 atcacagcgg cagccatgta cagcagatcc atattcaggt tttccatgac agtctccagt
    15181 ttgtttcagt taaaacgtag tagtgttggt aaattaatgt tcttcagacg ccatcgacag
    15241 atagacgatc gtcagaacca tgaagatgaa ggcttgcagc gtaatgatca ggatgtggaa
    15301 aatggcccac ggcacattca gaatccactg tgaccaccac ggcaacagac cagcaatcag
    15361 aatgaaaatc agctcaccgg catacatgtt accgaacagt cgcaaaccga gtgaaactgg
    15421 tttggacagc aggcttaccc cttcaaggat taagttgaca ggaatgaacg cccagtgatt
    15481 gaacggctgc agcgtcaact ctttcgtgaa gccgccgatg cctttcattt tgatgctgta
    15541 gaacagaatc aggataaata cacccagtgc catagacagc gttacgttca cgtccgcaga
    15601 cggaaccaca cgcagtgcag gcagacccag tacatgttca gcaatgtacg gcagcaggtc
    15661 gataggcagt aaatccatca ggttcatcag gaatacccag acgaagatcg tcagggccag
    15721 cggagcaatc agcttgcttt tgccatggta catgtctttc acgctaccat taacaaagcc
    15781 gatcaccagc tcaatcgcgg tctgaaactt acctggcaca ccgctggtcg cctttttggc
    15841 tacgctacgg aataaaacca ggaacaacag acccagcacc accgagaaga acatggagtc
    15901 aatattgatt gtccagaagg tggctggggg gttatgtgga tccaccagcg agaatgtacg
    15961 caggtccagc tgaaggttgt tcaggtggtg tcctatgtaa tcctgcggcg tcatattttc
    16021 tgaagccatg atgcctttta ccctttgttg ttaattacag ccggtgccag tatctgaacc
    16081 accagcacca aaacccacgt aacgatcagc ggcaagaata ccgcctttaa aaccgccaac
    16141 gccaccacca gtaacaccaa catcgccaga actttgaaag cttcgccaaa tgcgaatgtc
    16201 caggccaccc ggcctttcgc tggtgtatgc gcctggtgac gccaggcaaa tatcataaac
    16261 aaaacgttag gcagaaagac tgccaggccc ccgcttattg cagagacgcc ccagaagggg
    16321 tctttgaggc tgaacagcaa tccacttgct atcaccacca gtaactgaac gagcagaagc
    16381 ttccgagcaa cgtttcgact cacgagcgac acagacatca cgtttttcac tcctgctccc
    16441 ttcgaggtat gccgcgtgtc gtataaaact ttctttaagg cttagagtca agcatcaaaa
    16501 agcggtcaaa ttatacggtg cgcccccgtg atttcaaaca ataagtagcc aaaaggtgaa
    16561 taaatgttta aatatttttc cagtgcatac aattgcgact tttctgcaaa ccctgttcga
    16621 tcatgaaaaa ctgtaaataa cgcgtaaaca ctggtgataa agcgtgcttc agatcacata
    16681 ttgagcatgt tcgcgcacag catatttatt tacttggcaa atgatgcctt tgcaagttta
    16741 tgatatttca gtctaaaaac agatactgtt ttaataaatg acatttacac aacaaaaacc
    16801 acccattgac atttttaata atgttttaac agccaatgat gattcttagc gccgattttt
    16861 agcagactga tattttcact tatgacttat tttctgctta ccaaaaaaag ccacgttact
    16921 ttgttgatgc aaaagagtga acgtggcgtt aaatgtaacc agttatatca gtagaaaacc
    16981 tggttgttgt taacagtcta accggtcaat tttttatgat ttttttgata aaaattaaat
    17041 tttatttgct ttaatcacca ccagatgacg ttcgccatcc agggctggaa cctgaagttt
    17101 aaccactgat tcgacctgat attcttcggg caacaaagcg atttcatctt ccggcatttg
    17161 ccctttcagc gcgtagaaac ggccttgctc accaggaaga tggtggcacc agctcaccat
    17221 atcgttcaga gaggcaaaag cgcggctaat tacgccatca aatggcggct ctgaaggaaa
    17281 ctcttctacc ctgctctgta ctggctcaat attctccagt ttaagctcat gttgcacctg
    17341 acgaaggaaa cgcacgcgtt taccaaggct atccaacaac gtgaagtggg cttcaggacg
    17401 cacgatagag agtggaatgc caggcagtcc tggtccggtg ccgacatcga taaaccgttc
    17461 accttgcaga tacggtgcca ccacaatgct atcgagaata tggcgtacca gcatctcatt
    17521 aggatcgcgg accgaagtca ggttgtacgc tttattccat ttatgcagca tattcacata
    17581 ggcaataagc tggtttttct ggtgatcggt aagcgaaata cctgcgtctt tcagcagtaa
    17641 ggagagtttg ttgagcacgg tgattacctg ttcttgatgc gttgcccggt aagcgggtgc
    17701 ttaccaggca tttttaatgc gttatgcgct acgacgcagc ataccctgtt ttttcagcca
    17761 taccagcaga atggagatgg ccgcaggcgt gacgccagaa atacgcgaag cctggccgat
    17821 agaggccggt ttgtgatcgt taagtttggc gatcacttcg ttagaaagac cggatacctg
    17881 gcggtaatcc agcgtcgctg gtagcagggt gttctcgtta cgcagctgct tttcgatctc
    17941 atcttgctgg cgcgcgatat aaccttcgta tttaacctga atctcaacct gttccgccgc
    18001 ctgttcgtct gtcaacgcag gggcaaacgg cgtcagcgtg gttaattttt cataagtcat
    18061 ttccggacga cgcagcagat cttcaccact ggcttcacgg gaaagcggcg cagtcaggtg
    18121 agcattcact tcggctgcag cttccgccga cggggttacc caggtcgatt tcagacgctg
    18181 acgctcacgc tcgatattct caagtttctc gttaaagcgc gcccaacgtt cgtcatccac
    18241 caggcccagt tcacgaccga tttcagtcaa acgcagatcc gcattatctt cgcgcagcat
    18301 cagacgatat tctgcgcgcg aagtaaacat acgatacggt tctttggttc ctaaagtgca
    18361 caggtcatca accagtacgc cgagatacgc ctgagaacgc gccggagccc aaccttcttt
    18421 gtcagcagac agacgggcag cgttaagacc agccagcaaa ccttgcgcag cggcttcttc
    18481 gtaaccggta gtgccgttaa tctgaccagc aaagaacagc ccctggataa acttgctctc
    18541 cagcgtcggt ttcaggtcgc gtggatcgaa gaagtcatac tcaatggcat accccggacg
    18601 tacgatcttc gcgttttcca tcccctgcat ggagcggacg atttgcatct gcacatcgaa
    18661 cggcaggctg gtggagatac cgttcggata aatttcgttt gaggtcagcc cttccggttc
    18721 aaggaagatc tgatgctgat ttctgtcggc gaagcgcatg actttgtctt cgatcgacgg
    18781 gcagtagcgt gggccgacac cttcgatcac ccctgcatac attgggctac gatcgaggtt
    18841 actgcggatc acatcatggg ttttctcgtt ggtatgggtg atataacacg gcacctgctg
    18901 tggatgctgg gacgcattac ccataaacga gaataccggc attgggttat cgccatgctg
    18961 ttgcgccagc acgctgaagt cgatagtacg cgcatcaata cgcggcggtg tcccggtttt
    19021 cagacgacca acgcgcagcg gcagttcacg taaacggcga gagagcggaa tggacggcgg
    19081 atcaccagca cgaccaccgc tgtagttatc cagaccgata tgaattttac cgtcgaggaa
    19141 cgtcccaacg gtgagcacga cggctttggc acggaacttc agtcccattt gggtaacagc
    19201 accgaccacg cgatcgtttt cgacaataag atcttcaacc gcctgctgga agatcatcag
    19261 gttcggttgg ttctccagcg ccgtacgtac cgcctgccgg tagagcacac gatccgcctg
    19321 agcacgggta gcgcgaaccg ctggtccctt gcttgcgttt agtatcctaa actggatacc
    19381 cgcctgatcg atcgctttcg ccatcagacc gccgagtgca tccacttctt ttaccagatg
    19441 tcccttccca ataccgccga tcgccgggtt gcagctcatc tgccccagag tgtcgatatt
    19501 gtgtgtcaaa agcagagtct gttgacccat acgcgccgcg gccatcgcgg cctcggtgcc
    19561 tgcatgaccc ccgccaatga tgatgacgtc aaaaggatcc ggataaaaca tggtgattgc
    19621 ctcgcataac gcggtatgaa aatggattga agcccgggcc gtggattcta ctcaactttg
    19681 tcggcttgag aaagacctgg gatcctgggt attaaaaaga agatctattt atttagagat
    19741 ctgttctatt gtgatctctt attaggatcg cactaccttg tggataacaa ggatccggct
    19801 tttaagatca acaacctgga aaggatcatt aactgtgaat gatcggtgat cctggaccgt
    19861 ataagctggg atcagaatga ggggttatac acaactcaaa aactgaacaa ccgttgttct
    19921 ttggataact accggttgat ccaagctttc tgacagagtt atccacagta gatcgcatga
    19981 tctgtatact tatttgagta aattaatcca cgatcccagc cattcttctg ccggatcttc
    20041 aggaatgtcg tgatcaagga tgttgatctt cagagtttcg cctgtctgtt ttgcaccgga
    20101 atttttgagt tctgcctcga gtttatcgat agccccacaa aaggtgtcat attcacgact
    20161 gccaataccg atagcgccaa agcggaccgc agaaagatcg ggcttctgtt cctgcaatgc
    20221 ttcatagaaa ggagaaaggt tgtccggaat atctccggca ccgtgggttg agctgataac
    20281 cagccagatc cccgaggcag ataaatcttc caacagcgga ccgtgcagcg tttcggtggt
    20341 aaaacccgcc tcttccagct tttcagccag gtgttctgct acatattcgg caccgccgag
    20401 ggtgctgccg ctgataagag tgatatctgc cataaaccgc cacctttatt aagagtggcg
    20461 tattgtacgc tgtgaacgcg ttgggatcta cctgtggaaa agtatgggat taaaaaagcc
    20521 gatcagggct tgatggtacg catgatcggg ttctgcagga cgatcagtgt ctcggtggac
    20581 tgaatttcat caattgtttg gatcttgttg ataagtacat gctggagagc atcgatcgaa
    20641 cggcacatca ctttgataaa gatgctgtag tggccagttg tgtaataggc ttcagtgact
    20701 tcatcgaggc tttccagctt tgccagcgcg gaaggatagt ctttggcgct ctttaatata
    20761 atgccgataa agcagcctac gtcataaccg agctgcttcg ggctgacatc aatacgcgcc
    20821 ccggtaatga tccccgcctg cttcattttc tctactcgaa cgtgaattgt ccccggactg
    20881 acgccaaatt gtttcgccag ttcggcgtaa gcggtgcgcg cattgcccat taatgcttcc
    20941 aggatgccac ggtccagatt gtcgatcaga taattttcca taggattttc ttatgaggat
    21001 tgatgattca ttctatttta gccctatttt ttaatgaatc aaaagtgcat gaggcttttt
    21061 attgaatgat tattgcatgt gtgtcggttt ttgttgctta atcataagca acaggacgca
    21121 ggagtataaa aaatgaaaac cgcttacatt gccaaacaac gtcaaattag cttcgtgaaa
    21181 tctcactttt ctcgtcaact ggaagaacgt ctggggctga tcgaagtcca ggcaccgatt
    21241 cttagccgtg tgggggatgg cacgcaggat aacttgtcgg gctgtgaaaa agcggtgcag
    21301 gtaaaagtaa aagctctgcc tgatgcccag ttcgaagtgg ttcattcact ggcgaagtgg
    21361 aaacgtcaga ccttagggca acacgacttc agcgcgggcg aagggctgta cacgcacatg
    21421 aaagcccttc gccccgatga agaccgtctt tctccgttgc actcggtcta tgtagaccag
    21481 tgggactggg aacgcgtaat gggcgacggc gagcgtcaat tctcgactct gaaaagcacg
    21541 gtagaggcga tctgggcggg aattaaagca accgaagctg cggttagcga agagtttggc
    21601 ctggcaccgt tcctgccgga tcagatccac ttcgtacaca gccaggagtt actgtctcgt
    21661 tatccggatc tcgatgccaa agggcgtgag cgggcgattg cgaaagatct tggcgcggta
    21721 ttcctcgtcg ggattggcgg caagttgagt gatggtcatc gtcacgacgt gcgcgcaccg
    21781 gattatgatg actggagcac cccgtcagag ctgggccatg cgggtctgaa cggcgatatt
    21841 ctggtatgga acccggtact ggaagatgcg tttgagcttt cttccatggg gatccgcgtg
    21901 gatgccgata cgttgaagca tcagctggcg ctgaccggtg acgaagatcg cctggagctg
    21961 gagtggcatc aggcgctgct gcgcggtgaa atgccgcaga ccatcggcgg cggtatcggg
    22021 cagtctcgtt tgaccatgct gctgctgcaa ctgccacata tcggccaggt tcagtgtggt
    22081 gtatggccgg cagctgttcg cgagagcgtc ccttctctgc tgtaataatt tatcgccgcc
    22141 agcgtctgag caggcggctt cgcatcccgg tatcaaagcg ccagatatga tcgaaaatgc
    22201 gcatgatgcc gggtttgcca tgtgccgaca tcgccacggc atgaaagcga tgctgatgta
    22261 cccgctgtag ctctttcact ttactcgtca cgtcgtcagg caaccgctgg gcgataaaat
    22321 cagaaatcac caccgcatcg gcatcaaacc attcccggct ttgcaagcgt tccataatgg
    22381 cgcgaaaaca actggccaga tcggtgccgc cacgaaactg ctggcttaaa aaacggattg
    22441 cttgttcgat gccttgtggg cctgaaagct cataacggac gatctcggtg gaaaataaca
    22501 taatatagca gcgccggttt tcagcgagag caatgcgcat caaggccagg cagaacgctt
    22561 tcgcacactg ttcattaaag ccgcccattg agccggaagt atccacacag acaataaacg
    22621 gtccgcgcgg ctgttcgtcg taatctttat gtaccaccgg acgttcgatc actttttctc
    22681 gccacgactc accgtgcagg cgataggtga gcaactgttt ttccaccagc cgacggtaaa
    22741 actcatactc cagttctgtt atccctagtg tcgccagttc tggcggcagg agacgtaaaa
    22801 tatcatcgct ttgttgcaga ccatcaacct gctcaggaac cgtcgccggt tcgcgcacca
    22861 gggtgcggaa ggtttccatc tgcgcatcgt tgcgcggtat tgatttggct tcccgggaac
    22921 gccccagctg ttctgccagg cgtttcagtt ccggctgttc gttaagaaat tcaccgtatt
    22981 tcacaatcaa ctgatagtcg ccacgtttaa gctgaccggc gctcatatcc cacagacgac
    23041 cagctgcggt attgttatct gcgagaatcg gttcaagttg tccgctcagc gtcatgcgtt
    23101 cctgaacttc actcaacagt tgttcgcgtt cttcttctaa tagctgttga tttaacgtcg
    23161 ttgcttgcac gatcagactt aaacgccaac gctggagaaa aagcgtgtgt aacgctgaag
    23221 tgatcgtgct gttagcatca accaactgtc gggcttgttc tgcccatggg gaattcagac
    23281 gatgcagtaa gtccaggatc tgtggtagct gcacaataaa ctgcggcgtg gagaggagct
    23341 ggctttgctg atagcacatc acctcttcgg tgagttccgg cgggactcgg gcatctttca
    23401 gccgactgcg cagcgcctca cgccagcggg gaacatcatc agtgattgcc gccttcagtc
    23461 gtgggaattt ttcaaagaag actgccagct gcggtgaggc cagcagcgcg atgatcatct
    23521 cttcgatcaa tccctcttcg ctgacggcca gcatcacatt aagcgtatcc agcgttagca
    23581 ttgttgtgcc tggcgaatct gtgcgccgac atcctgtagg ctggcttcaa tgcgacctaa
    23641 ccagtcgcca gggataaaca gacatttttg ctgttcgctg aataaagcgt gttgcttgcg
    23701 ccagtcgctt tcaagctctt ccagttgttg ttttatttca cctggcagac cttcagccga
    23761 tgaaccggga agtgccagcg tactgccttg taaacttaca tcgcgtacaa caagatgttg
    23821 ggcgctatcc acttccagat tcagtttctg ggcaaagccg ataccgttca gtttgccgcg
    23881 aatctcacca cctttactca gccactgttc cagcgcgcta cgctcaaagg agatatgaac
    23941 cacttccata tcatgcaatt ttaacggttt ttgcagcagc agagtcagag tggaagcagt
    24001 aacgttaaca ggaagttgat actgctgacg acggctgaaa atgccgccca gacgaattac
    24061 cgttaaggct gttttatcgc tttgttgctg ctggagttgc aggtgacgtt gcacaatcgc
    24121 gccaaggcgg gtcaacatcc cttgctgttg ccaggcatga ccggtcatca atacatcaat
    24181 ttgttgttgt atcaaattca ggctttgcgc gtcataccac aggcaatctt tcagcaaaat
    24241 gagatcaacc ggggcaacag cactgcgacc gctaaaaaag gcgctggcct gcaataatcg
    24301 aatcgctttt ttccagcgac gatccgagac ataaggcgca tccggtaatt tatccagttg
    24361 ctggcgcagc ataaaaatca gctcaaatac atgatcgggc agcgtaattt caccaatctc
    24421 tttctgccag cgttcatatt cttcatctgt gacctgcaag gaggcaggaa ccggattgtc
    24481 gttttcatcc tgttgactgg tcagcatgga gcggaaattc gctttatcct gcactttatc
    24541 taaccacaga cgaatcagca tgcggtcata taacgcttcc agactgctgt ctgcttccgg
    24601 cagctcgttg gaggccgcca ccagcaggcg catcgggatt ttttctacgt gtgcaccgtt
    24661 acggaactgg cgctcgttaa tggcggtgag cagggtatta agaattgccg ggcccgcttt
    24721 ccagatctca tccagaaaga cgatttctgc ttccggcagg taaccgctgg ttaaacgttc
    24781 atagcgccct tcatctttta gcgcctgaat agaaagggga ccaaaaactt cttccggcgt
    24841 ggagaagcgg gtcatcagat attcaaacgc gcgggcattc tgaaaggcga attttaagcg
    24901 ccgggcgatc aaacttttgg caatacctgg cgggccaagg aggaacacac tttcaccact
    24961 taatgccgct aataaacaca agcggatggc gtggctacgt tcataaagcc ccttttccaa
    25021 cgaactgctc aggcgggaaa ttctttccgc taataaatga gggtgagcca taatgaagtg
    25081 gcgtcctttc gtcaaaagtt ctgcgtaaat tgcgagtata gacgtttctt gctggtggct
    25141 aaaatagtct caaagggggg gtatttttct ttgagccagg ttaatgtggc cgcatttagg
    25201 agtacgattt tgccgttaat cgtgcatact gtgcgctttt ttgtgggcca agggactaag
    25261 cacacatttc atatttcaac gaaagactag tctatgagca ctgataataa gcaatcattg
    25321 cccgcgatta ccctcgcggc gattggagtt gtctacggcg atattggtac cagcccgtta
    25381 tatacacttc gtgaatgttt gtccggccag tttggttttg gcgttgaacg cgatgccgtg
    25441 tttggctttt tatcgctgat cttctggctg ctaatctttg tggtttccat taaatatctc
    25501 accttcgtga tgcgggcaga taacgccggt gaagggggga tcctgacgtt gatgtcgctt
    25561 gccgggcgca atacgtcggc gcgaaccaca tcaatgctgg tgattatggg gctaatcggc
    25621 ggcagctttt tctatggtga agtcgtcata acacccgcta tttcggtgat gtcagccatt
    25681 gaaggtctgg aaatcgtcgc cccgcagctg gatacctgga tagttcctct ctcaattatc
    25741 gttctcactt tattatttat gattcaaaag catggcacgg ctatggtcgg taagctgttt
    25801 gcgccgatca tgctgacctg gtttttgatt ctggctgggc tggggttacg tagcattatt
    25861 gctaacccgg aagtgctgca tgcgctgaat ccgatgtggg cggtgcattt cttccttgaa
    25921 tacaaaacgg tttcttttat tgcattaggg gcagtggtgc tgtcgattac gggggtcgag
    25981 gcgctgtatg ctgatatggg gcactttggt aagttcccga ttcgtctggc gtggttcacc
    26041 gtcgtattgc cttccttaac tcttaattac ttcggtcagg gagcgctgtt gttaaagaac
    26101 ccggaagcaa ttaagaaccc gttcttcctg ttggcaccgg actgggcgct gatcccgctg
    26161 ctgatcatcg ccgcactggc gacggtaatc gcctcgcagg ctgttatctc tggcgtcttc
    26221 tcattgacgc gtcaggcggt acgtctggga tatttgtcgc cgatgcgcat tattcacacc
    26281 tccgaaatgg agtcagggca aatctatatt ccgtttgtga actggatgct ctatgtcgcg
    26341 gtcgtgattg tgattgtcag ctttgagcac tccagcaacc tggcggcggc gtacgggatt
    26401 gcggtgaccg gaaccatggt gctgacgtct attctctcga ctaccgtggc acgtcagaac
    26461 tggcactgga ataagtattt tgttgcgctg atcctgattg ctttcctttg tgtcgatatt
    26521 ccattgttca ccgctaacct cgataaactg ctctccggcg gctggttgcc attgagcctc
    26581 ggtacagtga tgtttatcgt gatgaccacc tggaagagcg agcgtttccg cttgctgcgg
    26641 cggatgcatg aacatggcaa ctctctggag gcgatgattg cttcgctgga gaaatcaccg
    26701 cccgttcgcg tgcccgggac cgcggtgtat atgtcacgtg caatcaacgt cattcccttt
    26761 gcgctgatgc ataacctgaa acataacaag gtattgcatg agcgggtgat tctgttaact
    26821 ctgcgcaccg aagacgcgcc atatgtccat aacgtccgtc gggtacagat tgaacaactg
    26881 tcgcccactt tctggcgcgt ggtggcaagt tatggttggc gagaaacgcc aaacgtggaa
    26941 gaagtattcc accgctgcgg tctggaagga ttaagttgcc ggatgatgga aacctccttc
    27001 tttatgtcgc atgagtcgtt gatcctcggc aaacgcccgt ggtatttgcg tctgcgcggt
    27061 aagctgtact tgctgttgca acgtaatgcg ctgcgtgcgc cggatcaatt tgaaatcccg
    27121 ccaaacaggg ttatcgaact gggtactcag gtcgaaatct aacgccagac gcctcctttc
    27181 tccataaggg ggcgtttttg ttttcatggt taatcaccat gtaaaacgtt tcgaggttga
    27241 tcacatttcc gtaacgtcac gatggttttc ccaactcagt caggattaaa ctgcgggtca
    27301 gcgaaacgtt tcgctgatgg agaaaaaaat gaaaaaaggc accgttctta attctgatat
    27361 ttcatcggtg atctcccgac tgggacatac cgatacgctg gtggtgtgtg atgcaggttt
    27421 acccatcccc aaaagtacaa cgcgtatcga tatggcatta acccagggtg taccttcttt
    27481 tatgcaggtg ctgggcgtcg tcacaaatga aatgcaggtc gaggcagcca ttatcgcgga
    27541 agagatcaaa caacataatc cgcaactcca cgaaacgttg ctcactcacc ttgagcagct
    27601 gcaaaaacac cagggaaata ccattgaaat tcgttacacc acgcatgaac aattcaaaca
    27661 acaaaccgca gaaagtcagg cggtaattcg cagcggagaa tgttctccgt atgcgaatat
    27721 cattctctgt gctggcgtga cgttctgagg ccgtcatgga agcattactt cagcttaaag
    27781 gcatcgataa agccttcccg ggcgtaaaag ccctctcggg tgcagcgtta aatgtctatc
    27841 ctggccgcgt gatggcgctg gtgggcgaaa acggtgcggg taaatccacc atgatgaaag
    27901 tgcttacggg tatctatact cgcgatgccg gtacgctttt atggctgggg aaagaaacga
    27961 cgtttaccgg gccgaaatct tcccaggaag ccgggattgg gattatccat caggaactga
    28021 acctgatccc gcagttgacc attgccgaaa acattttcct cggtcgtgag tttgttaatc
    28081 gctttggcaa aattgactgg aaaaccatgt atgccgaagc ggataaattg ctggctaaac
    28141 ttaacctgcg ctttaaaagc gacaagttag tgggcgatct ttccatcggt gaccagcaaa
    28201 tggttgaaat agccaaagtg ctgagctttg agtcgaaagt catcattatg gatgaaccga
    28261 ccgatgcgct gaccgatacc gaaaccgaat ccttgttccg cgtcatccgc gagctgaaat
    28321 cgcaaggccg cggtattgtc tatatttccc accgcatgaa agaaatcttc gagatttgcg
    28381 atgacgttac cgtttttcgt gatgggcaat ttattgctga gcgtgaagta gcatcactga
    28441 ccgaagattc gctgattgag atgatggtgg gccgcaagct ggaagatcaa tatccacacc
    28501 tggacaaagc gccaggagat attcgcctga aagtcgataa tctctgcgga cctggcgtta
    28561 acgatgtctc ttttacttta cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg
    28621 ctgggcgtac cgaactgatg aaagtgctct acggcgcact gccgcgcacc agcggttacg
    28681 tcaccctgga tgggcatgaa gtcgttaccc gttcaccgca ggatggcctg gcaaacggca
    28741 ttgtgtatat ctctgaagac cgtaaacgcg acggtttggt gctgggcatg tcagtaaaag
    28801 agaacatgtc gctgacagcg ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg
    28861 ccgatgaaca gcaggcagta agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga
    28921 tggaacaggc aattggtctg ctttccggtg gcaatcagca aaaagtggcg attgcccgcg
    28981 gtctgatgac acgccccaaa gtgttgatcc tcgatgagcc tacccgtggc gtagatgtcg
    29041 gcgcgaaaaa agagatctat caactgatta accagttcaa agccgatggc ttgagcatca
    29101 ttctggtgtc atcggagatg ccagaagtat taggcatgag cgatcgcatc atcgtcatgc
    29161 atgaagggca tctcagcggg gaatttgctc gtgagcaggc cacccaggaa gtgttaatgg
    29221 ctgccgctgt gggcaagctt aatcgcgtga atcaggagta aaaaaatgac aacccagact
    29281 gtctctggtc gccgttattt cacgaaagcg tggctgatgg agcagaaatc gcttatcgct
    29341 ctgctggtgc tgatcgcgat tgtctcgacg ttaagcccga actttttcac catcaataac
    29401 ttattcaata ttctccagca aacctcggtg aacgccatta tggcagtcgg gatgacgctg
    29461 gtgatcctga cgtcgggcat cgacttgtcg gtaggttctc tattggcgct gaccggcgca
    29521 gttgctgcat ctatcgttgg cattgaagtc aatgcgctgg tggctgtcgc tgccgctctc
    29581 gcgttaggtg ccgcaattgg tgcggtaacc ggggtgattg tagcgaaagg tcgcgtccag
    29641 gcgtttatcg ctacgctggt tatgatgctt ttactgcgcg gcgtgaccat ggtttatacc
    29701 aacggtagcc cagtgaatac cggctttact gagaacgccg atctgtttgg ctggtttggg
    29761 atcggtcgtc cgctgggcgt accgacgcca gtctggatca tggggattgt gttcctcgcg
    29821 gcctggtaca tgctgcatca cacgcgtctg gggcgttaca tctacgcgct gggcggcaac
    29881 gaagcggcaa cgcgcctttc tggtatcaac gtcaataaaa tcaaaatcat cgtctattct
    29941 ctttgtggtc tgctggcatc gctggccggg atcattgaag tggcgcgtct ctcctccgca
    30001 caacctacgg cggggactgg ctatgagctg gatgctattg ctgcggtggt tctgggcggt
    30061 acgagtctgg cgggcggaaa aggtcgcatt gttgggacgt tgatcggcgc attaattctt
    30121 ggcttcctta ataatggatt gaatttgtta ggtgtttcct cctattacca gatgatcgtc
    30181 aaagcggtgg tgattttgct ggcggtgctg gtagacaaca aaaagcagta ataacgacta
    30241 caggacatct tgagtatgaa catgaaaaaa ctggctaccc tggtttccgc tgttgcgcta
    30301 agcgccaccg tcagtgcgaa tgcgatggca aaagacacca tcgcgctggt ggtctccacg
    30361 cttaacaacc cgttctttgt atcgctgaaa gatggcgcgc agaaagaggc ggataaactt
    30421 ggctataacc tggtggtgct ggactcccag aacaacccgg cgaaagagct ggcgaacgtg
    30481 caggacttaa ccgttcgcgg cacaaaaatt ctgctgatta acccgaccga ctccgacgca
    30541 gtgggtaatg ctgtgaagat ggctaaccag gcgaacatcc cggttatcac tcttgaccgc
    30601 caggcaacga aaggtgaagt ggtgagccac attgcttctg ataacgtact gggcggcaaa
    30661 atcgccggtg attacatcgc gaagaaagcg ggtgaaggtg ccaaagttat cgagctgcaa
    30721 ggcattgccg gtacatccgc agcccgtgaa cgtggcgaag gcttccagca ggctgttgct
    30781 gctcacaagt ttaatgttct tgccagccag ccagcagatt ttgatcgcac taaaggtttg
    30841 aacgtaatgc agaacctgtt gaccgcacat ccggatgttc aggctgtatt cgcgcagaat
    30901 gatgaaatgg cgctgggcgc gctgcgcgca ctgcaaactg ccggtaaatc ggatgtgatg
    30961 gtcgtcggat ttgacggtac accggatggc gaaaaagcgg tgaatgatgg caaactagca
    31021 gcgactatcg ctcagctacc cgatcagatt ggcgcgaaag gcgttgaaac cgcagataaa
    31081 gtgctgaaag gcgagaaagt tcaggctaag tatccggttg atctgaaact ggttgttaag
    31141 cagtagtttt aatcaggttg tatgacctga tggtgacata aatacgtcat cgacagatga
    31201 acgtgtaata taaagaaaag cagggcacgc gccaccctaa cacggtggcg cattttatgg
    31261 acatcccgaa tatgcaaaac gcaggcagcc tcgttgttct tggcagcatt aatgctgacc
    31321 acattctgaa tcttcaatct tttcctactc caggcgaaac cgtaaccggt aaccactatc
    31381 aggttgcatt tggcggcaaa ggcgcgaatc aggctgtggc tgctgggcgt agcggtgcga
    31441 atatcgcgtt tattgcctgc actggcgatg acagcattgg tgagagcgtt cgccagcagc
    31501 tcgccactga taacatcgat atttctccgg tcagcgtgat caaaggcgaa tcaacaggtg
    31561 tggcgctgat ttttgttaat ggcgaaggtg agaatgtcat cggtattcat gccggcgcta
    31621 atgctgccct ttccccggcg ctggtggaag cgcaacgtga gcgtattgcc aacgcgtcag
    31681 cattattaat gcagctggaa tcaccactcg aaagtgtgat ggcagcggcg aaaatcgccc
    31741 atcaaaataa gactatcgtt gcgcttaacc cggctccggc tcgcgaactt cctgacgaac
    31801 tgctggcgct ggtggacatt attacgccaa acgaaacgga agcagaaaag ctcaccggta
    31861 ttcgtgttga aaatgatgaa gatgcagcga aggcggcgca ggtactgcat gaaaaaggta
    31921 tccgtactgt actgattact ttaggaagtc gtggtgtatg ggttagcgtg aatggtgaag
    31981 gtcagcgcgt tcctggattc cgggtgcagg ctgtcgatac cattgctgcc ggagatacct
    32041 ttaacggcgc gttaatcacg gcgttgctgg aagaaaaacc attgccagag gcgattcggt
    32101 ttgcccatgc cgccgctgcg attgccgtaa cacgtaaagg cgcacaacct tccgtaccgt
    32161 ggcgtgaaga gatcgacgca tttttagaca ggcagaggtg acgcttggct acaatgaaag
    32221 atgttgcccg cctggcgggc gtttctacct caacagtttc tcacgttatc aataaagatc
    32281 gcttcgtcag tgaagcgatt accgccaaag ttgaagcggc gattaaagaa ctcaattacg
    32341 cgccatcagc cctggcgcgt agcctcaaac tcaatcaaac acataccatt ggcatgttga
    32401 tcactgccag taccaatcct ttctattcag aactggtgcg tggcgttgaa cgcagctgct
    32461 tcgaacgcgg ttatagtctc gtcctttgca ataccgaagg cgatgaacag cggatgaatc
    32521 gcaatctgga aacgctgatg caaaaacgcg ttgatggctt gctgttactg tgcaccgaaa
    32581 cgcatcaacc ttcgcgtgaa atcatgcaac gttatccgac agtgcctact gtgatgatgg
    32641 actgggctcc gttcgatggc gacagcgatc ttattcagga taactcgttg ctgggcggag
    32701 acttagcaac gcaatatctg atcgataaag gtcatacccg tatcgcctgt attaccggcc
    32761 cgctggataa aactccggcg cgcctgaggt tggaaggtta tcgggcggcg atgaaacgtg
    32821 cgggtctcaa cattcctgat ggctatgaag tcactggtga ttttgaattt aacggcgggt
    32881 ttgacgctat gcgccaactg ttatcacacc cgctgcgtcc tcaggccgtc tttaccggaa
    32941 atgacgctat ggctgttggc gtttaccagg cgctgtatca ggcagagtta caggttccgc
    33001 aggatatcgc ggtgattggc tatgacgata tcgaactggc aagctttatg acgccaccat
    33061 taaccactat ccaccaaccg aaagatgaac tgggggagct ggcgattgat gtactcatcc
    33121 atcggataac ccaaccgacc cttcagcaac aacgattaca acttactccg attctgatgg
    33181 aacgcggttc ggcttagatt tacgctgtct tttgatcaaa ttattaccat cggttgtttt
    33241 cagaagcatg aacattgctg ctgaagcaac agtaataatg cccattgtga taaacgtata
    33301 gtggaattgt tcgacagtcg ttgtaccttc catcccttca taaacgcgaa ggacggccgc
    33361 acttacagca acgcctaaac tgatagacag ttgctgcgtg accgccagaa cactgttacc
    33421 gctgctggcg ttgtcatcgg tcagatcggc aagtgtgatg gtattcattg cggtaaattg
    33481 cgtcgacata gccatcccta atataaacaa cggcaagatc agcatccata tcgccattgc
    33541 tggtgattgc aaagagaact gagcgatcat tagcccaata atcaccgtga tccccactaa
    33601 cgtatggcga tagcccagac gacgtaagac ttgggtaacc atcgattttg caataatgga
    33661 acctaacgct gtcggtgcca tcatacagcc ggcaataaac gcctgataac caaatcctac
    33721 ctgtaacatc aatggcataa ggaacggtac acagccggtc cccagacggg ttgcaatatt
    33781 gcctacgata ccgatcgaga aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt
    33841 tggcgtgcgt cgtgcatgga gaatatagag aagcagtaac ccgatgctgg tgacaattac
    33901 cgtcaaggca atccaactgg cgacaatctt ttccccgaat agctctattc cgcttgagaa
    33961 gagaacaagg ctaaggccaa acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt
    34021 ggtgaaattg ggcatatgtt tgcgcgcgta aagaaggccc gcaataccta tggggatatt
    34081 gattaaaaat atccagtgcc aggttgccca ggtgaccagc acgccgccaa gaacggggcc
    34141 taaaattggc cccaccagac ccggcatggc gacaaaattc aatacaggaa gaagctcatt
    34201 acgaggataa gcgcgcagta aggccagccg agcaacaggc atcatcattg cgccgcctat
    34261 cccctgaata acccggaaga caaccagctg tggtagcgag ttagaaagtg cgcaggccag
    34321 agaacccaac gtgaacagac tcacggcaag ggtaaatatg cgacgcgtac cgaagcgatc
    34381 ggctagccat ccgcttaccg gaataagcat cgccaccgtc agcgtataac tgatgatggc
    34441 tgattgcatc gcgagaggag aacgattaag gctatgagcg attgcgggta aggcggtatt
    34501 cagaatagtg gcatcaagtg cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc
    34561 cgccatactg cgcttctttt tatcgctcat tcaatgtcct gttatcgggt tgtcacttat
    34621 caggtgagcg tagcagcgcc tgacaagctt taaatgccgc gtcgccatcg ctttggacaa
    34681 tcgcatcgac aatcgcctgg tgcagatcca gctttatcac tgtgtcgctg gtaattgacg
    34741 tgaagtaagt gtgataaacc gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg
    34801 cgctcatttc atagatatgc tcatgccaag ccatatcgac ctcaatccag cgttcacggc
    34861 gaaagttctc ttttaatgcc accatttcgg ccattaacgt attgagatgc gccttctgtt
    34921 ccgcggtgcc aaccgttgca gccagtaggc aagcttgcgg ctccagacag atacgcataa
    34981 ccagaaagtg atcgatgacc tgatgaaagt tctcttctgt catccaccag gtaagcaatt
    35041 cctgatcaag aaaattccag tttgattgtg gcatgacccg ggtaccaatt cgcggtcgcg
    35101 gtaaaaccat cccttttgcc gttaacgttt tgaccgcttc gcgtaccgct gtacgactca
    35161 ctccaaattg ctcgcccagc tcaatctcac caggcaaaat ggtgccgggt tcatattcac
    35221 cttttaagat gcgttgcgcc agcttctcag ccagaacata cgaaaggttt ttctgtgcag
    35281 ctaactgttg tgcgcttaaa ggcattacta atcttccttt ttctttttat tcctccttag
    35341 tatgccacca ggaagtgtga ttacggttgc aaaaacggca aattgcttgt tttatggcac
    35401 attaacgggg cttttgctga aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg
    35461 tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg gcaaacacgc
    35521 cgccgggtca gcggggttct cctgagaatc tcaacagaga aagcaaaaat aaatgcttga
    35581 ctctgtagcg ggaaag
//
LOCUS       DAOTRK010000035        35152 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.035, whole genome shotgun sequence.
ACCESSION   DAOTRK010000035 DAOTRK010000000
VERSION     DAOTRK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.035"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(315..716)
                     /gene="nikR"
                     /locus_tag="QIE41_001688"
     CDS             complement(315..716)
                     /gene="nikR"
                     /locus_tag="QIE41_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDS3530583.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(721..1527)
                     /gene="nikE"
                     /locus_tag="QIE41_001689"
     CDS             complement(721..1527)
                     /gene="nikE"
                     /locus_tag="QIE41_001689"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDS3530584.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALVVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAYPVRRRATEKV"
     gene            complement(1524..2288)
                     /gene="nikD"
                     /locus_tag="QIE41_001690"
     CDS             complement(1524..2288)
                     /gene="nikD"
                     /locus_tag="QIE41_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDS3530585.1"
                     /translation="MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2288..3121)
                     /gene="nikC"
                     /locus_tag="QIE41_001691"
     CDS             complement(2288..3121)
                     /gene="nikC"
                     /locus_tag="QIE41_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDS3530586.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3118..4062)
                     /gene="nikB"
                     /locus_tag="QIE41_001692"
     CDS             complement(3118..4062)
                     /gene="nikB"
                     /locus_tag="QIE41_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDS3530587.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4062..5636)
                     /gene="nikA"
                     /locus_tag="QIE41_001693"
     CDS             complement(4062..5636)
                     /gene="nikA"
                     /locus_tag="QIE41_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDS3530588.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5747..6334)
                     /gene="acpT"
                     /locus_tag="QIE41_001694"
     CDS             complement(5747..6334)
                     /gene="acpT"
                     /locus_tag="QIE41_001694"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDS3530589.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLSADSVQWIDSVN"
     gene            complement(6389..7438)
                     /locus_tag="QIE41_001695"
     CDS             complement(6389..7438)
                     /locus_tag="QIE41_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDS3530590.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHISPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7570..8787
                     /locus_tag="QIE41_001696"
     CDS             7570..8787
                     /locus_tag="QIE41_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3530591.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8791..9348)
                     /gene="dcrB"
                     /locus_tag="QIE41_001697"
     CDS             complement(8791..9348)
                     /gene="dcrB"
                     /locus_tag="QIE41_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDS3530592.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9421..10086)
                     /locus_tag="QIE41_001698"
     CDS             complement(9421..10086)
                     /locus_tag="QIE41_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDS3530593.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10308..10553
                     /gene="tusA"
                     /locus_tag="QIE41_001699"
     CDS             10308..10553
                     /gene="tusA"
                     /locus_tag="QIE41_001699"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDS3530594.1"
                     /translation="MTDLFSCPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10655..12853)
                     /gene="zntA"
                     /locus_tag="QIE41_001700"
     CDS             complement(10655..12853)
                     /gene="zntA"
                     /locus_tag="QIE41_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDS3530595.1"
                     /translation="MSTPDNHGKKAPHFAAFKPLTTAQNANNCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQTSRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVVLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAIALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPAVAFAGLINELESAGQTVVLVVRNNDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12927..13553)
                     /locus_tag="QIE41_001701"
     CDS             complement(12927..13553)
                     /locus_tag="QIE41_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDS3530596.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13694..14053
                     /locus_tag="QIE41_001702"
     CDS             13694..14053
                     /locus_tag="QIE41_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDS3530597.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14056..14325)
                     /locus_tag="QIE41_001703"
     CDS             complement(14056..14325)
                     /locus_tag="QIE41_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDS3530598.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14315..14911)
                     /gene="rsmD"
                     /locus_tag="QIE41_001704"
     CDS             complement(14315..14911)
                     /gene="rsmD"
                     /locus_tag="QIE41_001704"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDS3530599.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15061..16557
                     /gene="ftsY"
                     /locus_tag="QIE41_001705"
     CDS             15061..16557
                     /gene="ftsY"
                     /locus_tag="QIE41_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDS3530600.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAESEKAQPEAEVVAQPELVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWRAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16560..17228
                     /gene="ftsE"
                     /locus_tag="QIE41_001706"
     CDS             16560..17228
                     /gene="ftsE"
                     /locus_tag="QIE41_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDS3530601.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17221..18279
                     /gene="ftsX"
                     /locus_tag="QIE41_001707"
     CDS             17221..18279
                     /gene="ftsX"
                     /locus_tag="QIE41_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDS3530602.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18524..19378
                     /gene="rpoH"
                     /locus_tag="QIE41_001708"
     CDS             18524..19378
                     /gene="rpoH"
                     /locus_tag="QIE41_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDS3530603.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19649..20752
                     /gene="livJ"
                     /locus_tag="QIE41_001709"
     CDS             19649..20752
                     /gene="livJ"
                     /locus_tag="QIE41_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDS3530604.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20952..21335)
                     /gene="panM"
                     /locus_tag="QIE41_001710"
     CDS             complement(20952..21335)
                     /gene="panM"
                     /locus_tag="QIE41_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDS3530605.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            21759..22868
                     /gene="livK"
                     /locus_tag="QIE41_001711"
     CDS             21759..22868
                     /gene="livK"
                     /locus_tag="QIE41_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDS3530606.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            22916..23842
                     /gene="livH"
                     /locus_tag="QIE41_001712"
     CDS             22916..23842
                     /gene="livH"
                     /locus_tag="QIE41_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDS3530607.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23839..25116
                     /gene="livM"
                     /locus_tag="QIE41_001713"
     CDS             23839..25116
                     /gene="livM"
                     /locus_tag="QIE41_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDS3530608.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSISGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25113..25880
                     /gene="livG"
                     /locus_tag="QIE41_001714"
     CDS             25113..25880
                     /gene="livG"
                     /locus_tag="QIE41_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDS3530609.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25882..26595
                     /gene="livF"
                     /locus_tag="QIE41_001715"
     CDS             25882..26595
                     /gene="livF"
                     /locus_tag="QIE41_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDS3530610.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGNALLANEAVRSAYLGG"
     gene            26714..27460
                     /locus_tag="QIE41_001716"
     CDS             26714..27460
                     /locus_tag="QIE41_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS3530611.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            27820..29136
                     /gene="ugpB"
                     /locus_tag="QIE41_001717"
     CDS             27820..29136
                     /gene="ugpB"
                     /locus_tag="QIE41_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDS3530612.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            29312..30199
                     /gene="ugpA"
                     /locus_tag="QIE41_001718"
     CDS             29312..30199
                     /gene="ugpA"
                     /locus_tag="QIE41_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDS3530613.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSFIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            30196..31041
                     /gene="ugpE"
                     /locus_tag="QIE41_001719"
     CDS             30196..31041
                     /gene="ugpE"
                     /locus_tag="QIE41_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDS3530614.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            31043..32113
                     /gene="ugpC"
                     /locus_tag="QIE41_001720"
     CDS             31043..32113
                     /gene="ugpC"
                     /locus_tag="QIE41_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDS3530615.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            32110..32853
                     /gene="ugpQ"
                     /locus_tag="QIE41_001721"
     CDS             32110..32853
                     /gene="ugpQ"
                     /locus_tag="QIE41_001721"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDS3530616.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGDLNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWADMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32840..33280)
                     /locus_tag="QIE41_001722"
     CDS             complement(32840..33280)
                     /locus_tag="QIE41_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDS3530617.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSPLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33400..35142
                     /gene="ggt"
                     /locus_tag="QIE41_001723"
     CDS             33400..35142
                     /gene="ggt"
                     /locus_tag="QIE41_001723"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDS3530618.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPPPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLKK
                     GDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
BASE COUNT         8136 a         9140 c         9847 g         8029 t
ORIGIN      
        1 ccgaacaggc cacgccggtg ggggcaccaa tacgtacccc ggcagaaccc tgaacaatgc
       61 taccgccata ctgcgtcatg tcgccctgac gcgccgccgg ttttccgctc atcgtaaaat
      121 ccttttctat tattttaatg acagtatctt catacgaaaa agcgaagaaa aacttgtggt
      181 cacaatgaat tgaaggtttt taggaatata tttagaacgt tacaaaagag aaggttcgga
      241 tagggtattt agagaggaat caggtgtgct agtggccctt tgttgatcgt tcaccaaagg
      301 acccaataat aggcttaatc ttccttcggc aagcactgca aatgcccgtg ccgcacgccg
      361 cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcac ccatgtcacc tttcaacacg
      421 gcgatttcca gacagtcgtc gtgattgatg tggacatgta gcgtcgcgac ggagagatcg
      481 tggtgatggt gctgggtgga gacaatacgg ctggctaagt cgcgtttttc gtgttcatac
      541 acatacgaca gcaccgcgaa accttgtgtg ccgtgctgct gagtggcctc ttgcgccagg
      601 gcgctacgca gaatatcgcg gatagcttcg gaacggttgt tatagccacg acgctggctc
      661 aggctgtcca gcgtctccag taaatcgtca tcaagcgtga tggtaactcg ttgcatagac
      721 ttaaaccttt tctgtggcgc gacggcgcac ggggtatgcg ggtaataccg cgttttgtag
      781 cacacgtccg gcgtcagagg aaaaggttaa tttatctccc accacctggg tttcgacgat
      841 ttgcccgttg tccatcacca tcactcgctg gcaaaagcgt tccaccaggc gtaagtcatg
      901 agtgatgaac aggcaggcag tgccaaactg ttgttgtagc tttttcagca ggcgaatgac
      961 gcccgcctgt aacacgagat caaggttaga aacggcttca tccagaatca gcagtttcgg
     1021 ttcgaccacc aacgcgcgtg ccaggcagac gcgctggagc tggccgccgc ttaactgcgg
     1081 tgggcgcttg tcgagcacgc tgtcatcgag atcgaccgcc ttcagcattt cgctggcgcg
     1141 cgccagttgt tcggcttttt tcagcgatag caggtggcgc atcggttcac gcaggatctc
     1201 gcgcacggtt ttgcgcggat tcacggcact gatggagtcc tgaaacacca tctgaatatc
     1261 acggcggaag gctttacgtt gggcgcgatt aagtttcgcc agcgattcgc cgcgccagct
     1321 aatattccct tgtgagggag actctaagcc taccagcagc cgcgcgaggg tgctttttcc
     1381 gcagccgctg cgtcccagca gggcgacggt ttcaccgctt ttcagggtca gggaaacgtt
     1441 attcagtacc gcctgatgct gatgttttcc gctaaatcca ccgtgcgcat agtgatgtga
     1501 aaggtcgcag acgttaagta aagtcatgat gccagctcca taccgtagag ggcgagatga
     1561 gcggaaacca ggctgcgcgt caccgtatgt ttgggggcgt taaacagcgt ttctacatcg
     1621 ccctgttcga caattttacc gtgtgacatc accgccacgt catccgccag acgcgccaca
     1681 acgcccatat catgggtgac cagcagcatt cccggtgctt gtttttgcat aatgctttcc
     1741 agcagatcga ggatgcgcgc ctgtgctacc acgtcgaggt cggtggtcgg ttcatcggcg
     1801 atgataaacg gcgattcaca caacaccgcc atcgcaatca tcatgcgctg caacatgccg
     1861 ccgctcatct cgaacgggta cagcttcaac acgcgcgcgg cgttttccaa ccccaccgct
     1921 tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc ccagcgccag gcaggtttca
     1981 cgcgcgtggg tgtgcatggt gtgtagcgga ttaaaggcgc tgcgcgggtt ctgcatgatg
     2041 gtggcaattt tgatgccgcg cagggcacag ggagaaaccg gtttgccatc ggctaaaatt
     2101 tcccccgccg tctggcgaac gcctgcgggc agaatgccca gcgtcgcggc gcaggtcagt
     2161 gatttcccgc tgccgctacc gccgactaac gccagcacac gcccgcgttt aagggttaac
     2221 gatacgccgt gcaccagcgg ctgcgcggcc tgtagcgcga tatcgcgcag ttcaatctgt
     2281 tgcggcatca gtgtgcgtgc tccgtcacca gatgaggatc cagatgatcg cgcagtgcgt
     2341 cacccaccag gttaaaggcc atcacgctga taaacagcgc cagccccggc cagaacattt
     2401 gcagcggctg ggtccagata tactgacgcg cgtcgttaat catcacgccc cattcggcgg
     2461 tcggcgcggt cacgccgagg ccgaggaagg acatccccgc gacgtggagc atcatatgac
     2521 caatatccag cgtcgccagc accagcagcg aggggatcac cgcgcctgcc agatgatcga
     2581 caaacacccg cacatggccc gcgccggaaa gccgtgacgc cagcacaaac tcgcgctggc
     2641 gtagcgaaat caccaggctg cgcaccatgc gcgcatacca cgcccagtgc gacagggcga
     2701 tggcgataat cacgttggtc aacccggtgc cgagtacgcc gaccataaag aacgacagaa
     2761 tcgacgtcgg gaaggtcata aacatatccg cgacgcgcat ggtggcctga tcaacgcgcc
     2821 cgccaatcag cccggcgctg ccgccaataa ctagccctaa tgtcagcacc agcagcaggc
     2881 aggccattac cgagccgagc gacacgcggg tcgccgccat cagccgcgag aaaatatcgc
     2941 gccctaaatg atcggtaccc agccagtgct gcgcatccgg tgaaagcagg cgcgacggca
     3001 gatcaatcgc ctgggggtca tacggcagcc accactggct ggtgagcgca atcagcgcca
     3061 gcagggcgat aatgatcagc gccaggcgta ccgaccagcg ggaagagagg aaaaagttca
     3121 cgcgtgggct ccttcatgac gacgaatgcg cggatccagc gcggcgttga gcaaatcaac
     3181 aatcaaatta cagaccacaa aaaccaccac catcatcagc gtaaagcact ggatcaccgg
     3241 atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg acgcctggcc aggcaaagat
     3301 gttttcgata atcatcgtcc cgccaatcag ttcgccaatg tgcatcccca cggcggtgat
     3361 catcggcagc gaggcattgc gcagaatatg gcggcgttcg gtctgtttgt cgttcaggcc
     3421 gcgtagccgc gcccaggtga cgtgacgctg accggcgacg tccagcatac tggcgcgcag
     3481 tagccgtgca ttaatcgcca gcgacataaa ggcaatggaa accgcaggca aaatgatgtg
     3541 ctgccagccg ccgtagccca tcgcgggtag ccattgcaga tataccgaaa acgccatcac
     3601 cagcaaaaac gccagccaga agttgggcat cgacacgccg agaaacgcga tgaaacgtac
     3661 ggcgaaatcc ggcagacgat cgcgatggcg cgccgcccag ataccgagcg gcacggaagt
     3721 gagcagaatt aataccagcg cggcacctgc aagttccagc gtggcgggca ggaagttcag
     3781 catatcgtcc agcaccgggc gttggctggc gaatgagata ccaaagtcaa gatgcagcgc
     3841 cttccacaac caggtgccgt actggacata cagcggctga tccagcccca gcatggtgcg
     3901 tgtagaggcc agcatctccg gcgtcggcgg caggttagac agacgcagat aatcgagcgc
     3961 cgggtcgccg gtgccgaggc gcagcatcag aaaaatgatc accgaggcgg cgagcaccat
     4021 cgggatcagc agcagaaagc ggcgtaatac gtaacgcaac attaaggttt caccggttta
     4081 atctgttcga acggaatttc ggtggcgatc ggcgcgtagg ggatgttacc cagctccggt
     4141 tttgatacca ccatcattga gatgtaactg ataggcagat aaaccgcctc gtcatgcaga
     4201 cgggtcagaa tgtcgcgata cagcgcctga cgttgcgttt cgtcatgggt tgccagcact
     4261 tcgccgatct ctttatcaat cagcggcttg tcggctaatc cttgctgtgc ctggaaatca
     4321 gcgtgcgacg gtacgcgcat tgaactcagg aaggcgtgtg gatcgtatgg cgcgccccag
     4381 gtgcgatgga aaatcatgcc aaaacgaccg tcgcgctgac gagcatagat actgctctct
     4441 tcttcgccaa tcagtgagac atctgcgcca atctggcgca tatcagcctg aatgatttcc
     4501 gccatcgatt tgcttaacgc atcggtgccg atgaacgaca gttcaatgcg cagcggctga
     4561 ccatttttct cgcggatgtc tttgcccgca ggcaacgtcc aaccggcttt ttccagcaac
     4621 gctttcgctt tctgcggatc gtactggcgc ggtttcaggc cgaggttggc atagggcaca
     4681 gaaggagcaa acagggtgtc ggcgacctgc tgggtgccat acaacgcgtt atcaatcagc
     4741 gattttttgt ttaccgcgta attaagagct tcacgtactg ccagctcgtt ggtgggggct
     4801 ttggcggtat tgagcgccag catcacggtt tcgatcggct gtgacagttg ggtgtggtaa
     4861 gccgggttct ggctaaagcg ggcgaaggta tcgagcggta ataacccttc gtttccgtac
     4921 agcaggtcaa tatcgccagt ttcaaacgcc accgcgcggg tagtcgggtc cgggatcacg
     4981 ttaaaagtga tcttcttaat cgccggcttt tcgccccagt agttttcgtt acggacgaag
     5041 acatcgtact gattcagttt cgattcctgc aaaacccacg gcccggtgcc aatcggcgct
     5101 ttaattccgt tcatggtttc atggttttta aactgcgagg gggcgataaa acggaaaggg
     5161 cgtggcaggg ccagttcttg caggaaagga tagtaggcgc ttttcagggt gatttgcagc
     5221 tctgttttac tgagtgcttt gacatcaaca atctggtttg ccagctccag ccaggcgtga
     5281 cgttgacggt tatcgagcac tgcgcggaag ttttctgccg ccgcctctgc atcaaacggt
     5341 tcgccgttgg agaatttcac gtcatcacgc agggtgaagg tccaggtttt accatcttca
     5401 gaatgagtcc agctttttgc cagccacggg atcaccgaac cgtctgcctg atatttcacc
     5461 aatggttcat aaaccatgct ctgggcgaac atctggttag gcgtgtaaag gtgcgggttt
     5521 agtggcccga cattcaccgg ccaggcggtg gtgatttcat ctggtgcagc ggcatggacg
     5581 ataaaagacg cacaagccag cagcgcaaat agagtgcggc ggagtgtgga gagcatggta
     5641 accccaatgg attaaaatag atggcggaaa taagtatgac gattttaagt actcgtcata
     5701 ctgataacct gttctggatc aaaagatgga cagtcgggtg ggcggatcag ttaactgaat
     5761 cgatccattg cacactgtcg gcggagagcg taaagggggt aggggtgcag atcgccaggc
     5821 ttaaattatc aagctggcaa tggctgaccg acagtgatga gtgataggtg ctgtctacgc
     5881 tgacgatttg ccaggcgctg ccgccacgtt gtttaacgat ggcttctttg cgcgtccaga
     5941 tgcgccagaa catttccagt tgctgatcgg gatgcacggc gtccatctca gcgtgttccc
     6001 cgaggctgaa cacggcgttc gccagccagc gccagttggc gcgcgggcga attacttcga
     6061 tatcgcagcc gacttcgcct tcatcgctca acagcagggc gatatcgtca ccgctatggc
     6121 ttaagttgaa ccatagcggc gtttccggcg cgaatgcagg tttgccttgt tcgccataga
     6181 tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc acgccccgcc agccagcgtt
     6241 cacgtcgtgg accttgcggt gcttgctcgc gtaaacccgg tggcagagga gctgcgctta
     6301 aggtcgaaac tttccccaga actatccgat acatatcagg gccaacgttt aatggaaaat
     6361 gaaagtgcgt atcgtaacac ttgtcgcctc atcccggtaa ccgacttttc ggtctgcctg
     6421 gtcccagtaa aatcgccagt ttgctaccgc ctttggtggt ttccatccag attttacaca
     6481 cgctggttaa tggcaccgaa agtagcatcc ctaccgggcc gagcagccat ccccaaatta
     6541 acaatgaaag aaataccacc atggtggaca tccccaggcg atggcccatc atccgtggct
     6601 ctaaaatatt gccgatgacc atatggacaa ctaaaaacaa tgcgccgacc agaatacatt
     6661 cgtaaatacc attaaacagc agcacctgaa tcattggcgg tacggcggaa attaccgcgc
     6721 cgatattggg cacgtagttg agcaaaaacg ccagtactgc ccacatcagc gcaaactgta
     6781 cgcccattag cgccagcccc agccagacga tgacacctgt ccataaactg agtaacgtct
     6841 ttaacgccag ataatgcgaa acgcctttaa gtgcgcggtg taatcccgcg atgtgaatct
     6901 gtgggttatt tagcgcaaaa cgcattttgt aagggacgtg gcgcacttca aacagcatga
     6961 aaactacggt catcaccagc aaaagcacgc tcgccattgc cccggaaagc ccggtcatta
     7021 gcgccgtggt gaaggtaacc actttttccg agtccatccg ctgcagtatt cgctccggcg
     7081 atatatgcaa attaagaaaa ggcaacatct cctgcaattt aaaaagtttg cgcgtcagct
     7141 ccttattaaa cttcggcagc atagatataa attcgttaaa cgatgccgcc agtacgccga
     7201 ccagcgcggt tagtgcgatc agcatcacca ccaccacaat cgtaatggca acggggcgtt
     7261 gtaccccccg acgaataaac caggtgacga gcgggttgag aacgatggca aaaaacagcg
     7321 ccagtaatag ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg atcactacca
     7381 gcgaggccag cttgagcaga atgtgcatgc ccgttttatc gggttgaggg gtttccattg
     7441 gggcttcctt gtgacttttt gtattaagtg tagcgggagt cacgccagca atattctgat
     7501 tcttactgct gattttcacg tcagcacgtg gtaaaaatga aacactgttg taaaaatgtg
     7561 gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa acggattgcg cctgaatttg
     7621 cgcattgtct ccattgtcat gtttaacttc gccagctacc tcaccatcgg gttgccgctc
     7681 gctgtattac cgggctatgt ccatgatgtg atgggcttta gcgctttctg ggcaggattg
     7741 gttatcagcc tgcaatattt cgccaccttg ctgagccgcc ctcatgccgg acgttatgcc
     7801 gatttgctgg gacccaaaaa gattgtcgtc ttcggtttat gcggctgctt tttgagcggt
     7861 ctgggatatc tgacggcagg attaaccgcc agtctgcccg tcatcagcct gttattactt
     7921 tgcctggggc gcgtgatcct tgggattggg caaagttttg ccggaacggg atcgacccta
     7981 tggggcgttg gcgtggttgg ttcgctgcat atcgggcggg tgatttcgtg gaacggcatt
     8041 gtcacttatg gggcgatggc gatgggtgcg ccgttaggtg tcgtgtttta tcactggggc
     8101 ggcttgcagg cgttagcgtt aatcattatg ggcgtggcgc tggtggccat tttgttggcg
     8161 atcccgcgtc cgacggtaaa agccagtaaa ggcaaaccgc tgccgtttcg cgcggtgctt
     8221 gggcgcgtct ggctgtacgg catggcgctg gcgttggctt ccgccggatt tggcgtcatc
     8281 gccaccttta tcacgctgtt ttatgacgct aaaggttggg acggtgcggc tttcgcgctg
     8341 acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat tccctaacgg cattaaccgt
     8401 atcggcggct taaacgtagc gatgatttgc tttagcgttg agataatcgg cctgctactg
     8461 gttggcgtgg cgactatgcc gtggatggcg aaaatcggcg tcttactcgc gggggcaggg
     8521 ttttcgctgg tgttcccggc attgggcgta gtggcggtaa aagcggttcc gcagcaaaat
     8581 cagggggcgg cgctggcaac ttacaccgta tttatggatt tatcgcttgg cgtgaccgga
     8641 ccactggctg ggctggtgat gagttgggcg ggcgtcccgg tgatttatct ggcggcggcg
     8701 ggactggtcg caatcgcgtt attactgacg tggcgattaa aaaaacggcc tccggaacac
     8761 gtccctgagg ccgcctcatc atcttaaaac ttactgaata accagcgtat taatgatgtt
     8821 ttctgcggtg gtctgcgctt tttgctgatc gtcagctggc agagtaattt gcatggtcag
     8881 cagttgatta cccacgttac ccagaataac ggaagagtac gccgtctggc ctttcgcgga
     8941 gataatgctg tctaactgct gcattttgtg acctttcagc tcaatggctt tattggttac
     9001 cacttgcagc tgcggatcgc ggctacgttg ctgatcttcc agacgcttcg ccagcaccgc
     9061 cagatcttct ttcggatcat cgcccatgat gacgatgact gctttctgcc cggtggcgtc
     9121 ggaccagaca tgcatgttat tggcctgcgt tcccagctta ccgctctggt cggtcatatc
     9181 cgctggcagc gagaaactta acttgccatc aagcaggttg acgggattcc cggtagcgtt
     9241 actttccgcg accgaaccct gcgccgtagc gttagtgtct ttatcatcac aggccgcaag
     9301 ccccataacc agcaggccaa ttccgacata tttaaccaga ttgcgcattg acttcttcct
     9361 ttcgataaac ggccataacg gctcattcat ccatcttatc acaactctga taacgaacct
     9421 ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc agtcttttca acagcatatt
     9481 gagtaatacg ccatacattg gcaggaagaa aacgatactg attaacactt tgaaacagta
     9541 atcgaccagt gcgatttcca tccagtgttc agccataaag gcatccgggc tacgccagaa
     9601 ggcaatgaag aagaaggcca gcgtgtcgct gacgttaccg aacagtgtgg acgctgtcgg
     9661 tgccagccac cagcggcgac tctgacgcag gcggttaaaa acgtgcacgt cgaggatctg
     9721 tcccagcgcg taggccatga aactggcggt ggcgatacgg gcgacaaaca ggttgaagtg
     9781 ggcgagcgcg ccgaatcctt gccatgaccc catataaaac agcgatgaga tgacgtagga
     9841 gatcagcaac gccgggatca tcaccgcaaa gatgatacga cgggccagcg gtgcgccaaa
     9901 aatacgcaca gtcaggtcgg tagcaaggaa aataaacgga aagctaaatg cgccccaggt
     9961 ggtatggaaa ccaaaaatgg agacgggaag ctgaaccaga tagttactgg aggtgatcac
    10021 cagcagatga aataacgata accagaacaa cgccttatag cgttgagttt gcgagaaaac
    10081 gttcatattg tacctttttg attaaccatt ggggtgaggg aacccaatac gtacgacacg
    10141 ctattttacc ttgattaaat aacgttctta cccttttttc gagccgccgc atgatactgc
    10201 tttacgttga caatgcaatg gttaattttc acgcaatcgt taacctggtt tgcttacgga
    10261 ctcacagggc gtaaactagc gccgtttttt tatgtgatga gaagaaaatg accgatctct
    10321 tttcctgccc tgaccacaca ctcgacgcac ttggcctgcg ctgcccggaa ccagtcatga
    10381 tggtgcgcaa aaccgtgcgc aatatgcagc caggtgaaac gttgctgatt attgctgacg
    10441 atccggcgac gacccgcgat attcctgggt tttgtacctt tatggaacac gaactggttg
    10501 cgaaagagac ggatggactg ccttatcgtt atttgattcg caaaggcggt tgatggggaa
    10561 agattggctt cgatgccgcc ttttcccctc accctaaccc tctccccaga ggagcgaggg
    10621 gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct cctgcgcaac aatcttaacg
    10681 cattcgctgt caccagcacc gtcgcacccg tatctgccag caccgccagc cacaggccgg
    10741 tcatccctaa cagtgtggtg acgaggaaga tccctttcag ccccagcgca atagtgatgt
    10801 tctggcggat attggcgtga gtggcgcgtg ccagttcaat catttgcacc aggccgcgca
    10861 ggtggttatg ggttaatgct gcatcggcgg tttccagcgc cacgtctgtg ccactgccca
    10921 ttgcaatccc gatggcggca gctttcatcg ctggcgcgtc gttaatacca tcaccgacca
    10981 tcgccagcgg cgcatgttga ttcagctcgg tcaccgcttt gactttatct tccggcaaca
    11041 ggcccgcttt aaattccagc cccagttccc cggcaattgc cgccgctgcg cgtggattat
    11101 cgccggtgag gatcacccct ttgacgccca gcgcgttcag ttcactgatg gcagttgcag
    11161 catcggcgcg cagagtatcc tgcaatgcaa tgatacccag cacgtcattg ttacgtacta
    11221 ccagcactac cgtttgcccg gcgctttcca gttcgttaat taaaccagca aatgcaacag
    11281 ccggatgttt tccggcagcg catatcaaca cgcgctcacc gttaacctgc gcttcaatgc
    11341 cagacccaac cagcgcccgc tgtgattcgg cggcgggaat ggcgagttca gcaacctgtg
    11401 cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc ttgctcgacc gccgctgcca
    11461 gtgtcagcag ttcagattca ctaatacccg ttgccggatg aatcgcggta acgcgcggtt
    11521 taccgacggt cagcgtaccg gttttatcaa acgccacctg ggtaacacga cctagttgtt
    11581 ccagcgccgc accgccttta atcaacgccc cacgacgcgc tgctgctgcc agcccggagg
    11641 ttatcgccgc aggcgttgag ataactaacg cacacgggca accaatcagc agcagcgtca
    11701 gccctttata aatccactct tgccagctgg cggcaaacag cagcggcggc accagcgtta
    11761 ccagcagggc gatggccata atcgctggcg tgtaaatacg gctgaaacgg tcgataaacc
    11821 tctcaatggg ggcgcgacgc tcttcggctt cttcgatcag tttcagaatc cggtcaatgg
    11881 cgctggcacc cggttctgac agcacttcca gcgttaccag acggtctacg ctggtggctc
    11941 ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat ggattctccg gtcagggcgc
    12001 tttcatcaaa actggcaaac ggtgagagca gtttaccgtc ggcaggcaaa cgcccgcccg
    12061 ccgcaacttc aatcacatcg ccagggcgca ggctgttaat cgccacctct tcccgctcac
    12121 cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat caacgcgcta acgccctgac
    12181 gcgcgcggct ggcggcccag ccttccagtc gttcaccaat caaaaacagc agcaacacca
    12241 tcgcagcttc agccgttgcg ccaataaaca gcgcaccaat agcagctacg ctcattaacg
    12301 tttcaatggc gaagtaactg ccggatttga tcagccgtaa tgcctgacga gcaatcgggt
    12361 acagcacaac cagcgtggtc gcgataaacg ccagttgccc gaacggatga ttaaactgct
    12421 ccagacccca gctgattgcc atcatcacga ttagcgtaat cagcggcaaa ttctctttca
    12481 ggcgtgatgt ttgcggttcg tcggcggcct gttcatcgcg cagggaatag cccgcttttt
    12541 gcaccgcaga ttcaacctgg gcacggatgt cattgtcggc atcgaccacc agtttttcgg
    12601 tggcgaacaa cacctgcacc tgattcacgc ctgcaagctg gcgcacggca ttttctacct
    12661 tgcgcgcaca ggcggcgcag tccatgccgc tgactttcca gctatagcgg gtgccggaga
    12721 cgttttcaga gagcgttggc gtgctggaac atgcgccgtc gcagcaacag ttgttggcgt
    12781 tctgtgccgt ggttagcggt ttgaacgcag caaagtgagg ggctttcttg ccgtgattgt
    12841 caggagtcga catggcatcc tccggttaag ttttttctca ttaaccgaag gatacactct
    12901 ggagtcgact ccagagtcaa gttttatcag agatacagcg agcggacgat caggaagtga
    12961 ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac ggaagcgtcg gcgatagtgg
    13021 ctccccagcc agacaaagtt actgataaat agcaacgaag cgccaacaaa cgcagagagc
    13081 gccggagcgg tcggacggaa gaaccacagt tcacctgcca gccacaccat caccagcgtc
    13141 atgccgataa acgtgcagat aggccaacgg tactcttcca gccgcgtcca gataatcgcc
    13201 agtaacagcg cccccagaac cagcaacacc agtggcagag gccagaagaa agagagcgtc
    13261 atctgactgg cgaaatagat ggtgtacagc aggtgcgaga ggaaaaacgc gccgatggcg
    13321 tacatcagac gttgacgtgg caacagggtt agcgcatcgc ccagcagtga ggcgcacagc
    13381 cccgccagca ccagatagct aatggcgtcg aacatcggcg cttgccaggc cagtaacagc
    13441 aggagaagaa gggttaacgg tttaaacacc cagcgttgcc aggttggccc acgatacgat
    13501 gcatccacag atagccatgc ggaaagacag acagcgataa acgaccaaag catcttagtt
    13561 ccttgaattc gttatttttt cgtaagcgga atgcctacgg tcttgcttcc agtttagtgg
    13621 cgagggtgga cggatgacaa caccataact ggcagggtat gcttaattcc gcattttccg
    13681 atgagggata aagatgagca aaccacctct tttctttatt gttatcattg gcttaatcat
    13741 cgtcgccgca tcgtttcgtt ttatgcaaca acgacgggaa aaagctgata atgatatggc
    13801 accgctccag caaaagttgg tggtggtgag caacaagcgg gaaaaaccga ttaacgatcg
    13861 ccgttcgcgc cagcaggaag tgactccggc aggcaccagt atgcgctatg aggcaagctt
    13921 caaaccgcaa agcggaggaa tggagcagac gtttcgcctc gacgcccagc agtaccacgc
    13981 cctgacagtg ggcgataaag gtacgctgag ctataaagga acgcgctttg tcagctttgt
    14041 tggcgaacaa taaggttatt ttttaacttt aaatttcttc tgccagacca gcagttcaaa
    14101 cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc atctgcgggc catctttcgg
    14161 caaggtcgat ttcaatagcg ccagctgcat accatgcatc agcacggtaa aaatcagcgc
    14221 cacgttaaca aaaatattca gcgggcgtgg gaagggatgc accaggttga ggattaaaaa
    14281 tccccaaacg cagagcatta acaaacgacc aatattaatc agcatcactt tctccttgtg
    14341 cttcgcgttg atacagccga taagccacct gacccgccac tttttcccga tgtaatgacc
    14401 agtttgccgg aacagtgggc agaccgtttt cgacttcgct ttcgacataa atcagggctt
    14461 cgtcagccag ccagccgtta tcttccagta aatttatcgt ctcttctaac aagccacggc
    14521 ggaacggcgg atcgacaaac acgatattat gcggtgtgcc tttttgcgcc aggaatgaca
    14581 tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag tgtcgccaga ttcttaatta
    14641 actgctgaga aaccgcgcga tccatctcaa tcaacgtggc ccccgcagcg tagcgcgata
    14701 acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc cagacattgg gcgtcaacaa
    14761 tgaccggagc cagccagtta aacaacgttt cgcgtacgcg gtcggtggtg gggcgcagac
    14821 ctgggctatc aggaaccggg agtttacggc ctcgccactg cccgccaata atgcggattt
    14881 ggccgctgcc ggaatgattc ggttttttca tgataaattg ctcaatccgc cagataacac
    14941 atacttgcag gcggtgatgt gaattaagtt aagtgataga ctatttcatc atttttttag
    15001 ctgctatgta catagcgtta acgctgtgcc atgaagcaac agcgaggagt gtagtcgcaa
    15061 atggcgaaag aaaaaaaacg tggctttttt tcctggctgg gctttggtca aaaagagcag
    15121 accccggaaa aagagacaga agttcagaat gaacaaccgg ttgtagaaga aatcgttcag
    15181 gcgcaagagc ctgcgaaggc ttcggaacaa gccgttgaag agcagccgca ggcgcatact
    15241 gaggccgaag cggaaacttt tgctgccgaa gttgtggaag tcactgaaca ggttgctgaa
    15301 agtgaaaaag cgcagcctga agcggaagtc gttgcacagc cggaactggt cgcagaagaa
    15361 acgccggagc cagtggctat cgaacgtgaa gagctgccgt tgccggaaga cgtcaacgcc
    15421 gaagaggttt cgccagaaga gtggcgggct gaagcggaaa ccgtagagat tgtcgaagcg
    15481 gcggaagaag aagcggctaa agaagaaatc accgacgaag agctggaagc acaggcgctg
    15541 gctgccgaag cggcagaaga ggcagtgatg gtggttcctc cggcagaaga agagcagccg
    15601 gtagaagaaa tcgctcagga gcaggaaaaa ccgaccaaag aaggtttctt cgcgcgcctg
    15661 aaacgcagcc tgttaaaaac taaagaaaat ctcggttccg gatttatcag cctgttccgc
    15721 ggtaaaaaaa tcgacgatga tctgtttgaa gagctggaag aacagctgtt gatcgccgat
    15781 gtgggcgtgg aaaccacacg taaaataatc accaatctga cggaaggcgc atcccgcaag
    15841 cagcttcgtg atgccgaggc gctctatggc ctgctgaaag aagagatggg cgagattctg
    15901 gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc cgttcgtgat cctgatggta
    15961 ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc tggcgcgtca gtttgagcag
    16021 cagggtaaat cggtgatgct ggcggcgggc gatactttcc gtgcggcagc ggttgaacag
    16081 cttcaggtct ggggtcagcg caacaatatt ccggtgattg cccagcatac cggtgcggat
    16141 tccgcctctg ttatcttcga cgccattcag gccgctaaag cgcgtaatat cgacgtcctg
    16201 attgccgata ccgccggacg cctgcagaac aaatcgcacc tgatggaaga gttgaagaaa
    16261 atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc atgaagttat gctgactatc
    16321 gatgccagca ccgggcagaa cgcggtaagc caggccaaac tgttccatga agccgttggc
    16381 ttaaccggca tcacgctaac gaaactggac ggcacggcga aaggcggggt aattttctcg
    16441 gtggctgacc agtttggtat tcctatccgc tacattggtg tcggcgaacg tattgaggat
    16501 ttgcgtccgt ttaaggcgga cgactttata gaggcacttt ttgcccgaga ggattaacaa
    16561 tgattcgctt tgaacatgtc agcaaggctt atctcggtgg gagacaggcg ctgcagggcg
    16621 ttacgttcca tatgcagccg ggtgagatgg cgtttctgac cggtcattcc ggcgcaggga
    16681 aaagtaccct cctgaagctg atctgtggga ttgagcggcc cagcgccggg aaaatctggt
    16741 ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt tccgtttctg cgccgccaga
    16801 ttggcatgat tttccaggat caccatctac tgatggaccg tactgtctac gataacgtgg
    16861 cgatcccgct gattatcgct ggtgccagcg gtgacgatat tcgtcgccgg gtgtcggcgg
    16921 cactggataa agtcgggcta ctggacaaag cgaagaactt ccctattcag ctttcgggcg
    16981 gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa taagcccgcg gtactgctgg
    17041 cggacgaacc gactggtaac ctggacgacg cgttgtcgga aggcatttta cgtctgtttg
    17101 aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac gcacgacatc aacctgattt
    17161 cgcggcgttc ctatcgcatg ctcaccctga gcgatggtca cttgcatgga ggcgtgggcc
    17221 atgaataagc gcgatgcaat caatcatatt cggcagtttg gcgggcgtct ggatcgcttc
    17281 cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg cgccaaaacg cgcgaaatcc
    17341 tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg aacaggtgcg ctatgccttc
    17401 cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca cgtttttaac ggtgatggtt
    17461 atcgccatct ccctgacgct gcccagcgtc tgttatatgg tgtacaaaaa cgttaaccag
    17521 gcggcgacgc aatattatcc gtcaccgcaa atcactgttt atctgcaaaa aacgctggac
    17581 gatgacgccg ctgcgggtgt ggtggcacag ttgcaggccg agcaaggcgt ggagaaagtg
    17641 aactatcttt ctcgtgaaga cgcgctgggc gagttccgta actggtctgg ttttggtggt
    17701 gcgctggata tgctggaaga aaacccgctt ccggcagtgg cggtggtgat cccgaaactc
    17761 gattttcagg ggacagaatc actgaatacg ctgcgtgatc gtatcaccca gatcaacggc
    17821 attgacgaag tgcggatgga tgacagctgg tttgcccgtc tggcggcgtt aaccgggctg
    17881 gtcgggcgcg tttccgcgat gatcggcgtg ttgatggtgg cagcagtgtt tctcgtcatc
    17941 ggcaacagtg tgcgtctgag catctttgct cgccgtgact ccattaacgt gcagaaactg
    18001 attggtgcga cggatggttt catcctgcgc ccgttcctgt acggtggcgc actgctggga
    18061 ttttctggcg cattgctgtc attaattttg tcagaaattc tggtactgcg tttgtcatcg
    18121 gcggttgcgg aagtggcaca ggttttcgga acaaagtttg atatcaatgg cttatcgttc
    18181 gatgaatgcc tgctgttgct gctggtatgc tcgatgattg gctgggtggc agcgtggctt
    18241 gccacggtac aacatttacg ccactttacg cctgaataat aaaagcgtgt tatactcttt
    18301 ccctgcaatg ggttccgtag cagggaaaga gaccccgttg tctcttcccg gtatttcatc
    18361 tctatgtcac attttgtgcg taatttattc acagcgttgc attgaacttg tggataaaat
    18421 cactgtctga taaaacagtg aatgataacc tcgttgctct taagctctgg cacagttgtt
    18481 gctaccactg aagcgccaga agatatcgat tgagaggatt tgaatgactg acaaaatgca
    18541 aagtttagct ttagccccag ttggcaacct ggattcctac atccgggcag ctaacgcgtg
    18601 gccgatgttg tcggctgacg aggagcgggc gctggctgaa aagctgcatt accatggcga
    18661 tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg tttgttgttc atattgctcg
    18721 taattatgcg ggctatggcc tgccacaggc ggatttgatt caggaaggta acatcggcct
    18781 gatgaaagca gtgcgccgtt ttaacccgga agtgggtgtg cgcctggtct ccttcgccgt
    18841 ccactggatc aaagcagaga tccacgaata cgttctgcgt aactggcgta tcgtcaaagt
    18901 tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg cgtaaaacca agcagcgtct
    18961 gggctggttt aaccaggatg aagttgaaat ggtggcccgt gaactgggcg taaccagcaa
    19021 agacgtacgt gagatggagt cgcgtatggc ggcacaggac atgacctttg acctgtcttc
    19081 cgacgacgat tcggatagcc agccgatggc tccggtgctc tatctgcagg ataaatcatc
    19141 taactttgct gacggcatcg aagatgataa ctgggaagag caggcggcaa accgtctgac
    19201 cgacgcgatg caaggtctgg acgagcgtag ccaggatatc atccgcgcgc gctggctgga
    19261 cgaagacaac aagtccacgt tgcaggagct ggctgaccgt tacggtgttt ccgctgagcg
    19321 tgtgcgccag ctggaaaaga acgcgatgaa aaaacttcgc gctgccattg aagcgtaatt
    19381 tccgctacta agcagagaac cctggatgag aatccggggt ttttgttttt tgggcctctg
    19441 caataatcag tttcccctcc ggcaaaacgc caatccccac gcagattgtt aataaactgt
    19501 caaaatagct attccaatat cataaaaatg gggtatgttt tagcagagta tgctgctaaa
    19561 gcacgggtag tcatgcataa aacgaaataa agtgctgaat cacaacacca caacacacgt
    19621 aataaccaga agaatgggga acctcaggat gaacatgaag ggtaaagcgt tactggcagg
    19681 atgtatcgcg ctggcattca gcaatatggc tctggcagaa gatattaaag tcgcggtcgt
    19741 gggcgcaatg tccggtccgg tggcccagta cggtgaccag gagtttactg gtgctgagca
    19801 ggcggttgcg gatatcaatg ctaaaggtgg tatcaaaggc aacaaactgc aaatcgtgaa
    19861 atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg gcgaacaaag tcgttaacga
    19921 cggcattaaa tatgtgattg gtcacctctg ttcttcatca acgcagccag cgtcagacat
    19981 ctatgaagat gaaggcattt taatgatcac cccagcggca accgcgccag agctgaccgc
    20041 tcgtggctat cagctgatcc tgcgcactac cggcctggac tccgaccagg ggccgacggc
    20101 ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt gctatcgtcc acgacaaaca
    20161 gcagtacggt gaaggtctgg cgcgtgcggt gcaggacggc ctgaagaaag gcaatgctaa
    20221 cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat ttctctacgc tggtggcgcg
    20281 tctgaaaaaa gagaatatcg acttcgttta ctacggcggt tatcacccgg aaatggggca
    20341 aatcctgcgt caggcacgcg cggcggggct gaaaactcag tttatggggc cggaaggcgt
    20401 ggctaacgtc tcgctgtcta acattgcggg cgaatcagcg gaagggctgc tggtgaccaa
    20461 gccgaagaac tacgatcagg ttccggcgaa caaacccatt gttgacgcga tcaaagcgaa
    20521 gaaacaggac ccaagcggcg cgttcgtctg gaccacctac gccgcgctgc aatctttgca
    20581 ggcgggtctg aatcagtctg acgatccggc tgaaatcgcc aaatacctga aagcgaactc
    20641 cgtggatacg gtgatggggc cgctgacctg ggatgagaaa ggcgatctga aaggctttga
    20701 gttcggcgta tttgactggc acgccaacgg cacggcgacc gatgcgaagt aatcattaat
    20761 cggcaacttt gggttgccgc caaattgcta atatcgagta cgttgcttca tgccggatgc
    20821 ggcgtaaacg ccttatccgg cctacaagat ccaaagaaat cagtaaattg caacacacgt
    20881 tgtaggcctg ataagcgtag cgcattaggc aatttctcgt tggtcatcac ttttgaaatc
    20941 aaactttacg attaacgctt ctcccagccg ccctgttgtg ccgtaaatcc cagtgcctgc
    21001 ataaacgctg tcatcacgcc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa
    21061 acgccaggat tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg
    21121 gtgacttccc taacacgcag ggaatccagt gccccctcgg tgccgcttaa ggttacccgc
    21181 acggccgcga gaaggcgctc gttaaaacgc gcggcgtaga tacggtggtt atcgtcaacc
    21241 tgtaaagagg aaggggaata ctccggccag atcttttgca ggtcaatccg gtcctggtcg
    21301 ctaaagtttt ctaatcgaat gatggtcagc ttcatgggga acccgtgtaa atcacaaaag
    21361 tggaaccagt gtagcgaaat aatttaatcg gaggctttct cttttttatt tcttttggca
    21421 ggtgattaat tttttaacag caataattac aaagttaaaa cattatagaa tgaaaaatac
    21481 ccagcataat cccctgaatg atagtgaatt attccgccct ttgtgccgtt attttatgct
    21541 gacaacggca cttttttctg tttatctatc attaaattca gaatattatc tgttcttaat
    21601 cgactgaaaa atagggattt taatcgctat tatcacaaaa tactgcgcta aatccttaat
    21661 cagacaggca aaaacagtgc agtataaaaa aagaacagtc tgatttgtta acacataaaa
    21721 acaaagcaac acaacatcac gaatggggat ttttgactat gaaacggaat gcgaaaacta
    21781 tcatcgcagg gatgattgca ctggcaattt cacacaccgc tatggctgac gatattaaag
    21841 tcgccgttgt cggcgcgatg tctggcccga ttgcccagtg gggcgatatg gaatttaacg
    21901 gcgcgcgtca ggcgattaaa gacattaatg ccaaaggggg aattaaaggc gataagctgg
    21961 ttggcgtgga atatgacgac gcctgcgacc cgaaacaagc cgttgcggtc gccaacaaaa
    22021 tcgttaacga cggcattaaa tacgttattg gtcatctgtg ttcttcctct acccagccag
    22081 cgtcggatat ctacgaagac gaaggtattc tgatgatctc accgggagcg accaacccgg
    22141 aactgaccca acgcggttat caacacatca tgcgtactgc cgggctggac tcttcccagg
    22201 ggccaacggc ggcaaaatac attcttgaga cggtgaagcc ccagcgcatc gccatcattc
    22261 acgacaaaca acagtatggc gaagggctgg cacgttcggt gcaggacggg ctgaaagcgg
    22321 ctaacgccaa cgttgtcttc ttcgacggta ttaccgcggg tgagaaagat ttctccgcgc
    22381 tgatcgcccg cctgaaaaaa gaaaatatcg acttcgttta ctacggcggt tactacccgg
    22441 aaatggggca gatgctgcgc caggcccgtt ccgttggcct gaaaactcag tttatggggc
    22501 cggaaggtgt gggtaatgcg tcgttgtcga atattgctgg cgatgctgcc gaaggcatgt
    22561 tggtcaccat gccaaaacgc tatgaccagg acccggcaaa caaaggcatc gttgatgcgc
    22621 tgaaagcaga caagaaagat ccatccgggc catatgtctg gatcacctac gcggcggtgc
    22681 aatctctggc aactgccctt gagcgtaccg gcagcgatga gccgctggcg ctggtgaaag
    22741 atttaaaagc taacggtgca aataccgtga ttgggccgct gaactgggat gaaaaaggcg
    22801 atcttaaggg atttgatttt ggtgtcttcc agtggcacgc cgacggttca tccacggcag
    22861 ccaagtgatc atcccaccgc ccgtaaaatg cgggcgggtt tagaaaggtt accttatgtc
    22921 tgagcagttt ttgtatttct tgcagcagat gtttaacggc gtcacgctgg gcagtaccta
    22981 cgcgctgata gccatcggct acaccatggt ttatggcatt atcggcatga tcaacttcgc
    23041 ccacggcgag gtttatatga ttggcagcta cgtctcattt atgatcatcg ccgcactgat
    23101 gatgatgggc attgataccg gctggctgct ggtggcggcg ggattcgtcg gcgcaatcgt
    23161 cattgccagc gcctacggct ggagtattga acgggtagcc tatcgcccgg tgcgtaactc
    23221 taagcgcctg attgcgctca tctcggcaat cggtatgtcc atcttcctgc aaaactacgt
    23281 cagcctgacc gaaggttcgc gcgacgtggc gctgcccagc ctgtttaacg gtcagtgggt
    23341 ggtgggccat agcgaaaact tctctgcctc tattaccacc atgcaggcgg tgatctggat
    23401 tgttactttc ctcgccatgc tggcgctgac gattttcatt cgctattccc gcatggggcg
    23461 cgcctgtcgt gcctgcgcgg aagatctgaa aatggcgagt ctgcttggca ttaacaccga
    23521 ccgggtgatt gcgctgacct ttgtgattgg cgcggcaatg gcggcggtgg cgggtgtgtt
    23581 gctcggccag ttctacggcg tcattaaccc ctatatcggc tttatggccg ggatgaaagc
    23641 ctttaccgct gcggtgctcg gtgggattgg cagcattccg ggagcgatga ttggcgggct
    23701 gatcctgggg attgcggagg cgctctcttc tgcctatctg agtacggaat ataaagatgt
    23761 ggtctcattc gccctactga ttctggtgtt gctggtgatg ccgaccggta ttctgggtcg
    23821 cccggaggta gagaaagtat gaaaccgatg catattgcaa tggcgctgct ttctgccgcg
    23881 atgttctttg tcctcgctgg cgtctttatg ggcgtgcaac tggagctgga tggcaccaaa
    23941 ctggtggtcg acacggcttc agacatccgt tggcagtggg tgtttatcgg cacggcggtg
    24001 gtctttttct tccagctttt gcgaccggct ttccagaaag ggttgaaaag catttccgga
    24061 ccgaagttta ttctgcccgc cattgatggc tccacggtga agcaaaagct gtttctcgtg
    24121 gcgctgctgg tgcttgcggt ggcgtggccg tttatggttt cacgtgggac ggtggatatc
    24181 gccaccctga ccatgatcta cattattctc ggtctcgggc tgaacgtggt ggtaggtctt
    24241 tctggtctgc tggtgctggg atacggcggc ttttacgcca tcggcgctta cacttttgcg
    24301 ctgctcaatc actattacgg tttgggcttc tggacctgtc tgccgattgc cgggttaatg
    24361 gcagcggcgg cgggcttcct gctcggcttc ccggtgttgc gtttacgtgg cgactatctg
    24421 gcgatcgtta ccctcggttt cggcgaaatt gtgcgcatat tgctgctcaa taacaccgaa
    24481 attaccggcg gaccgaacgg tatcagtcag atcccgaaac cgacgctctt cggactcgag
    24541 ttcagccgta ccgcccgtga aggcggctgg gacacgttca gtaatttctt tggcctgaaa
    24601 tacgatccct ccgatcgcgt tatcttcctg tacctggtgg cgttgctgct agtggtgtta
    24661 agcctgtttg tcattaaccg cctgctgcgg atgccgctgg ggcgtgcgtg ggaagcgttg
    24721 cgtgaagatg aaatcgcttg tcgttcgctg ggcttaagcc cgcgtcgtat caagctgact
    24781 gcctttacca tcagcgctgc gtttgccggt tttgccggaa cgctgtttgc tgcgcgccag
    24841 ggctttgtca gccctgaatc cttcactttt gccgaatcgg cgtttgtgct ggcgatagtg
    24901 gtgctcggcg gtatgggctc gcaatttgcg gtaattctgg cggcaatttt gctggtggtg
    24961 tcgcgcgaat tgatgcgtga tttcaacgaa tacagcatgt taatgctcgg tggtttgatg
    25021 gtgctgatga tgatctggcg tccgcagggc ttgctgccca tgacgcgccc gcaactcaag
    25081 ctgaaaaacg gcgcagcaaa aggagagcag gcatgagtca gccattatta tctgttaacg
    25141 gcctgatgat gcgcttcggc ggcctgctgg cggtgaacaa cgtcaatctt gaactgtacc
    25201 cgcaggagat cgtctcgtta atcggcccta acggtgccgg aaaaaccacg gtcttcaact
    25261 gcctgaccgg attctacaaa cctaccggcg gcaccatttt actgcgcgat cagcatctgg
    25321 aaggtttacc ggggcagcaa atcgcccgca tgggcgtggt gcgcaccttc cagcatgtgc
    25381 gtctgttccg tgaaatgacg gtgattgaaa acctgctggt ggcacagcat cagcaactga
    25441 aaaccgggct gttctccggc ctgttgaaaa caccctcttt ccgtcgcgcc cagagtgaag
    25501 cactcgaccg cgccgccacc tggcttgagc gtattggttt gctggaacac gccaaccgcc
    25561 aggcgagcaa cctggcctat ggcgaccagc gtcgtctgga gatcgcccgc tgcatggtga
    25621 cgcagccgga gattttaatg ctcgacgaac cggcagcagg tcttaaccca aaagagacga
    25681 aagagctgga tgagctgatt gccgaactgc gtaaccatca caacaccact atcttgttga
    25741 ttgaacacga tatgaagctg gtgatgggga tttcagacag aatttacgtg gtcaaccagg
    25801 ggacgccgct ggcaaacggt acgccggagc agatccgtaa taacccggac gtgatccgtg
    25861 cctatttagg tgaggcataa gatggaaaaa gtcatgttgt cctttgacaa agtcagcgcc
    25921 cactacggca aaatccaggc gctgcatgag gtgagcctgc atatcaatca gggcgagatt
    25981 gtcaccctca ttggcgcgaa cggcgcgggg aaaaccacct tgctcggcac gttatgcggc
    26041 gacccacgcg ccaccagcgg gcgaattgtg tttgatgata aagacattac cgactggcag
    26101 acggcgaaaa tcatgcgcga agcggtggcg attgtcccgg aagggcgtcg cgttttctcg
    26161 cggatgacgg tggaagagaa cctggcgatg ggcggctttt ttgccgaacg cgaccagttc
    26221 caggagcgca taaagtgggt gtatgaactg tttccacgtc tgcatgagcg ccgtgttcag
    26281 cgggcaggca ccatgtccgg cggtgagcag cagatgctgg cgattggtcg cgcgctgatg
    26341 agcaacccgc gtttgttgtt acttgatgag ccatcgctcg gtcttgcgcc gattatcatc
    26401 cagcaaattt tcgacaccat cgaacaactg cgcgagcagg ggatgactat cttcctcgtc
    26461 gagcagaacg ccaaccaggc gctgaagctc gctgatcgcg gctacgtgct ggaaaacggc
    26521 catgttgtgc tctccgatac tggtaatgcg ctgctggcga acgaagcagt gagaagtgcg
    26581 tatttaggcg ggtaaattat cgataacccg gcagctaagt tctgccgggt tattcgtccg
    26641 tatacagact tgtttcaact gaccaggagg attaaaacca gaccacttat ttgccacgga
    26701 aaggttccct cccatgcatt atgccgttgt tgtcatgctt gaactgcttt gctgtattca
    26761 cgcttaccgt accggacaag agcggtactg gatttttatc attttctgtt ttccggtgat
    26821 tggctgcgtg gcgtatttcg tcatagtgat gctgcctgaa acgggcgctg accgtcacgg
    26881 gcatactttg ctgatgcgcc tgcaggacaa actcaaccct gagcgccacc tgcgcaaatt
    26941 aaccgaagaa ctggcgattg ccgagaccaa tcaaaaccat tatgcgttgg ctaatgaact
    27001 ggcgcgtctg gggcgttatc acgaagctgt tccccattat cagcaggcgt taagcgggat
    27061 attcgcccat gaagcggcaa tgatgctaag cctggcgcag gcacagtttg ctattcagga
    27121 gttcgccgcc tgtcagcaga cgctggaaga tgtgatgcgt tataaccctg attttcagtc
    27181 ggcagacggg catttgttat ttgccagaac gttagccgca caagaaaaat acgctgatgc
    27241 ggaaagcgag tttgaagtat tgatttctta ttaccccggc ccgcaggcgc gtatttacta
    27301 tgcggaaatg ttggcgaaaa tgagtcggtt acgtgaagcc aacgagcagt atgttgccgt
    27361 ggttgatacg gctaaacgct caagacctca ttatagaaaa caccaccggg aatggattaa
    27421 gaccgccaat gagcggctga agcagagtgt ggtgcaatga aaatcttctt tgccgttaga
    27481 ccagtgtcgc ggcaatcttt tgttaaaatt gaaaaatgta gctctcatcg ggcagcaaaa
    27541 ctggacgcag aatttgctgc catttttgct cttcatggta aggagttctg gcgattagct
    27601 ggccgttaat cgcctctttt cagtgcattc tcgggatatc cgtgccccat ctcccatttt
    27661 ccctcacccg taccgtcacc gccttgtcat ctttctgaca ccttactatc ttacaaatgt
    27721 aacaaaaaag ttatttttct gtaattcgag catgtcatgt taccccgcga gcataaaacg
    27781 cgtgaattcg cgcattcggt acaacaagag agataaacga tgaaaccgtt acattataca
    27841 gcttcagcac tggcgctcgg actggcgtta atggggaatg cacaggcagt gacgaccatt
    27901 ccgttctggc attctatgga aggggaactg ggtaaagagg tggattctct ggcccaacgt
    27961 tttaacgccg aaaacccgga ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag
    28021 aatttaagcg cggggattgc cgcatttcgt accggcaacg cgcctgctat tttgcaggtt
    28081 tatgaagttg gcaccgccac catgatggcg tcgaaagcca ttaaaccggt atatgacgtg
    28141 tttaaagagg cggggattca gttcgatgag tcgcagtttg tgccgacggt ttcaggctac
    28201 tactccgaca gcaaaaccgg ccacttactc tcccagccgt tcaacagctc gactcccgtt
    28261 ctctattaca acaaagacgc cttcaagaaa gccggtttag acccggaaca gccgccgaaa
    28321 acctggcagg atctggcgga ctatgccgca aaactgaaag cctccggtat gaagtgcggc
    28381 tacgccagcg gctggcaggg ctggatccaa ctggaaaact ttagcgcctg gaacggtctg
    28441 ccgtttgcca gcaaaaacaa cggctttgac ggcacagacg cggtgctgga gttcaacaag
    28501 ccggagcagg tgaaacacat cgctatgctc gaagagatga acaagaaggg cgatttcagc
    28561 tacgtcgggc gtaaggatga atccactgag aagttctata acggtgattg cgcgatgacc
    28621 accgcctctt ccggttctct cgccaacatt cgcgagtacg ccaaatttaa ctacggcgta
    28681 ggcatgatgc cttacgacgc cgatgcgaaa gatgcgccac aaaacgccat tatcggcgga
    28741 gccagcttgt gggtaatgca gggtaaagat aaagaaactt atacgggtgt ggcgaagttc
    28801 ctcgacttcc tcgcgaagcc agaaaacgct gccgagtggc atcagaaaac tggttatctg
    28861 ccaatcacca aagcggcgta tgacctgacc cgtgagcagg gtttttatga gaaaaaccca
    28921 ggggcggata ccgcgacgcg tcagatgctg aataagccgc cgttaccgtt caccaaaggg
    28981 ctgcgtctgg gcaacatgcc gcagatccgc gtgattgtgg atgaagagct ggagagcgtg
    29041 tggaccggta agaagacacc acagcaggcg ctggataccg ccgttgagcg tgggaaccag
    29101 ttactgcgcc gctttgagca gtctacgaag tcttaattct cttaccctca ccctaaccct
    29161 ctcccaaagg gagaggggac cgatcggcgt ggtatccagg tgccgtgtcg gtttgcaact
    29221 tcgctcggtg ggaaagtatg caccgccttt ctccctcgcc cctttgggga gagggccggg
    29281 gtgaggggaa cacaacacga agagtttaac tatgtcatca tcccgtccgg tgttccgctc
    29341 gcgctggctg ccctatttgc tggtcgcgcc gcagctcatt atcaccgtta tcttttttat
    29401 ctggcctgcg ggcgaagcgt tgtggtactc gctgcaaagc gtcgatccgt ttggtttctc
    29461 cagccagttt gtcggcctgg ataacttcgt cgcgctgttt catgacagct actatctcga
    29521 ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt tgctggtgtc
    29581 gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc agccgtttct atcaaacctt
    29641 aatgctgctg ccttacgccg tggcacccgc cgttgccgcc gtattgtgga tcttcctgtt
    29701 taaccctggt cgcgggctga tcacccattt tctcgctgag ttcggctacg actggaacca
    29761 cgcgcaaaac agcggtcagg cgatgtttct ggtggtgttt gcctcggtgt ggaagcaaat
    29821 tagctacaac tttctgttct tctatgccgc gctgcaatcc attccccgtt cgtttatcga
    29881 agccgcagcc atcgacggtg caggcccgat tcgccgcttc tttaagattg cgctgccgct
    29941 tatcgctccg gtgagtttct tcctgctggt ggtgaacctg gtgtacgcct tcttcgacac
    30001 cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca cgacgctgat
    30061 ttataagatt taccgcgaag gctttaccgg actggatctg gcttcgtctg ccgcgcagtc
    30121 ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg cagttccgct atgttgaaag
    30181 caaggtgcgt taccaatgat tgagaaccgt ccgtggctga caatattcag ccataccatg
    30241 ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg tggcgtttgt cgcggcgacg
    30301 ctggataaac aggccgtcta cgccgcgccg atgacgctaa tccccggcac acatctgctg
    30361 gaaaacatcc acaacatctg ggtgaacggg gtaggcacga atagtgcgcc tttctggcgg
    30421 atgttgctta acagctttgt gatggcgttc agcattacgc tcggcaaaat taccgtctcg
    30481 atgctttcgg catttgccat tgtctggttt cgttttccgc tacgtaacct cttcttctgg
    30541 atgattttta tcactctgat gttgccggtt gaagtgcgta ttttcccgac ggtggaagtg
    30601 atcgccaatc tgaaaatgct cgacagctac gccggtttaa cgctgccgct gatggcctcg
    30661 gcgaccgcca cgtttctttt ccgccagttc tttatgacgc tgccggatga gctggtggaa
    30721 gcggcgcgga tcgacggtgc gtcaccaatg cgcttctttt gcgacatcgt ttttccgctc
    30781 tccaaaacta atctggcggc gctgtttgtg atcaccttta tctacggctg gaaccagtat
    30841 ttgtggccgt tgttgattat taccgatgtg gatctcggca ccaccgtggc agggattaaa
    30901 gggatgatcg ccactggcga aggcaccacg gaatggaact cggtgatggc ggcgatgttg
    30961 ttaacgctga tccctccggt ggtgattgtt ttagtgatgc agcgtgcctt tgtgcgcggc
    31021 ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa ccaaaagctg
    31081 ggatggcaaa acccaggtca ttaaaccgct gacccttgat gtggcggatg gcgaatttat
    31141 cgtgatggtc gggccgtctg gctgcgggaa atcgacgtta ttgcgaatgg tggccggact
    31201 ggagcgggta acgacgggcg atatctggat cgaccgcaag cgtgtcaccg aaatggagcc
    31261 gaaagatcgc gggattgcga tggtgttcca gaactacgcg ctctatccgc atatgagcgt
    31321 agaggagaat atggcgtggg ggctgaaaat tcgcggcatg ggcaagcaac aaattgccga
    31381 acgtgttaaa gaagcggcgc gcattctgga gctggacggc ctgcttaagc gccgcccgcg
    31441 tgagttatct ggcggtcagc gtcagcgtgt ggcgatgggg cgtgcgattg tgcgtgagcc
    31501 ggcagtgttc ctgtttgatg agccgctctc taacctcgat gccaagttgc gtgtgcagat
    31561 gcgtcttgaa ctgcaacagc tgcaccgtcg cctgaaaacg acttcactct acgttaccca
    31621 cgatcaggtt gaggcgatga cgctcgccca gcgagtaatg gtgatgaacg gcggcgttgc
    31681 cgaacagatt ggcacgccag ttgaagtgta cgaaaagccc gccagcctgt ttgtggcgag
    31741 ttttatcggc agtccggcga tgaatctgct ggcagggcgt gtgaataacg aaggtacgca
    31801 tttcgaactg gacggcggta tcgcgctgcc gctaaacggt ggctaccgtc agtatgctgg
    31861 acgtaaaatg actctcggca ttcgcccgga acatattacg ctacgttcgc aggctgaagg
    31921 tggcgtgccg ctggtgatgg acacgctgga gatcctcggc gcagataacc tggcgcacgg
    31981 acgctggggc gaacagaagc tggtggtgcg actggcgcat caggagcgcc cgacggcagg
    32041 cagcacgctg tggctgcatc tgccggaaaa tcagctgcat ctttttgatg gtgaaacagg
    32101 acaacgagta tgagtaactg gccttatccc cgcatcgtcg cccatcgtgg cggcggtaaa
    32161 ctggccccgg agaacaccct ggcagcaatc gacgtcgggg caaaatacgg tcataagatg
    32221 atcgaatttg acgcgaagtt atcgaaagat ggcgagatct tcctgctcca tgacgacaat
    32281 cttgaacgca ccagcaacgg ctggggcgtc gcgggcgatc tgaactggca ggatttactg
    32341 cgcgtggatg caggcagttg gtacagcaaa gcgtttaaag gtgagccgct accgttgctt
    32401 tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg cgaatatcga aatcaaaccc
    32461 accactggca ccgggccatt aacgggcaaa atggtggcgc tggcggcacg cgaactgtgg
    32521 gccgatatga cgccgccgct gctgtcatcg tttgagattg atgctttaga agctgcacaa
    32581 caggctgcac cggaactgcc gcgcggtttg ttgttggatg agtggcgcga cgactggcga
    32641 gaactgaccg cgcgactggg ctgcgtctct attcatctca atcataagtt gcttgataaa
    32701 gcgcgagtga tgcagttgaa agacgcagga ttacggattc tggtttatac cgtcaataaa
    32761 ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgcac cgatgcgatt
    32821 gacgtgattg gtccgaactt tacggcccaa tagttttcaa cggaatatca ggctgcggcg
    32881 tgctgggctg attcagcatg tcgccgtttc tcctctccgg caacatatgc tgctgattca
    32941 acggactatc cggattacgg ttgctgttta acatcccgcc gttggtgttg ggcagcattt
    33001 gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga gaattattgt
    33061 tgatctggtt ttctaaatgc tgctgttgca actgcgtttg cgttttcagt tgctgattca
    33121 gcatcccttt ttgctggatt tgctgcgttt gcatttgagt ctgcatccgc tgctggctgg
    33181 ggatctgata tcccggctgg ttggggttgt tcagagtatt aatgggctgt gcaaagccga
    33241 caaacggcag gagtgccgtc aaaagcagaa gtcgtttcat cgcgtatcct cctctgaaga
    33301 tattctttaa gtttactcgc ttcccggcaa aacgatgatt aattcagagt tatataccag
    33361 gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc gacgttttta
    33421 cgccgggtag ccattgctgc tctgctctca ggaagttgtt ttagcgccgc cgccgcgcct
    33481 cctccgccac ctgtttctta tggcgtggag gaagatgtct tccatccggt acgcgcgaaa
    33541 caggggatgg tggcgtctgt agatgctact gccactcagg tgggcgtgga tatcctcaag
    33601 gagggcggga atgccgttga tgctgccgtg gcggtgggct acgcgctggc ggtaacgcat
    33661 ccgcaggcgg ggaatctggg cggcggtggt tttatgctga tccgctcgaa aaatggcaat
    33721 accaccgcta tcgatttccg cgaaatggca cccgccaaag cgacccgcga tatgttcctt
    33781 gatgatcagg gtaacccgga cagcaaaaaa tcactcactt cgcatctggc ctccggtacg
    33841 cccggtacgg tagcaggttt ctcgctggcg ttggataaat acggcaccat gccgttgaac
    33901 aaagtggtgc aaccggcgtt taaactggca cgcgatggtt ttatcgttaa cgacgcgttg
    33961 gctgacgatc tcaaaaccta cggtagcgaa gtgttgccga atcacgaaaa cagcaaggct
    34021 atcttctgga aagagggcgc gccgctgaaa aagggcgaca agctggtgca ggcgaacctg
    34081 gcaaagagcc tggagatgat tgccgaaaac ggcccggacg aattctacaa aggcacgata
    34141 gcggaacaga ttgcccagga gatgcagaaa aacggtggct tgatcactaa agaagattta
    34201 gcggcgtata aagcggtcga acgtacaccg attagcggcg attatcgcgg gtatcaggtt
    34261 tactccatgc caccgccatc ttccggcggg atccatatcg tacaaatcct caatattctg
    34321 gaaaacttcg atatgaagaa atacggcttt ggcagcgccg acgcgatgca aatcatggca
    34381 gaagctgaga aatatgccta cgccgaccgc tcggaatatc ttggcgaccc ggattttgtc
    34441 aaagttccgt ggcaggcgct gaccaataaa gcctatgcca aatctattgc cgatcaaatc
    34501 gatatcaata aagcgaagcc atccagcgaa attcgccccg gcaagcttgc gccttatgag
    34561 agtaatcaaa ctacccatta ctcagtggtg gataaagacg gcaacgcggt agcggtgacc
    34621 tatacgctga acaccacctt cgggacgggc attgtcgcgg gcgagagcgg tattctgctt
    34681 aataaccaga tggatgattt ctccgccaaa cctggcgtac cgaacgttta cgggctggtg
    34741 ggtggtgacg ccaacgcggt cgggccgaat aaacgcccgc tgtcgtcgat gtccccaacc
    34801 attgtggtga aagacggtaa aacctggtta gtgactggta gcccaggcgg tagccggatc
    34861 atcactaccg tgctgcaaat ggtggtgaac agcatcgatt atggtatgaa tgtcgccgaa
    34921 gcgaccaatg caccgcgttt ccaccatcag tggttgccgg acgagctgcg tgtcgaaaaa
    34981 gggtttagcc cggatacgct caagctgctg gaagcaaaag gtcagaaagt ggcgctgaaa
    35041 gaggcgatgg gtagtacaca aagcattatg gttgggccgg acggcgagtt gtacggcgca
    35101 tccgatccgc gctcggtgga tgatttaacg gcgggttatt aaggttagcg gc
//
LOCUS       DAOTRK010000036        35037 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.036, whole genome shotgun sequence.
ACCESSION   DAOTRK010000036 DAOTRK010000000
VERSION     DAOTRK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.036"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            36..434
                     /locus_tag="QIE41_001724"
     CDS             36..434
                     /locus_tag="QIE41_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530619.1"
                     /translation="MSFQGDESTQKTLKEAYKAVAETKFGHKITEELESSEHEYIFRG
                     LRKGINQTCYDDTEYSFYIDIDNDHSSCVYQGKNKACAMKPTLLSVVLAHEMGHAKGM
                     KDDGTDSMANVDKYENPFRKELGLPARMKY"
     gene            564..902
                     /locus_tag="QIE41_001725"
     CDS             564..902
                     /locus_tag="QIE41_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530620.1"
                     /translation="MPFRIQTYSPVTKSKIENFAIKDGAQKLLQINREKLLMLLNRSS
                     NECYHFDDKNVRIYIIDGKDRYYIDKNGVVSYGVQDNVICSNNKINEDSIEKILLEGN
                     IDFWKKLKIK"
     gene            1355..1792
                     /locus_tag="QIE41_001726"
     CDS             1355..1792
                     /locus_tag="QIE41_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530621.1"
                     /translation="MADKAILWALISASTQEGRKACSLSYFSCKAAEAELGLAYMAAN
                     DNKAFLTSLSRIMMYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPHQLVADCIETVNK
                     IKDKNKKIIDIDSVNICNDNKNINWRVNSTIVAIDDSIKCIDE"
     gene            complement(2250..3386)
                     /locus_tag="QIE41_001727"
     CDS             complement(2250..3386)
                     /locus_tag="QIE41_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDS3530622.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLSEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(3389..3751)
                     /locus_tag="QIE41_001728"
     CDS             complement(3389..3751)
                     /locus_tag="QIE41_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDS3530623.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            4288..6201
                     /gene="mtlA"
                     /locus_tag="QIE41_001729"
     CDS             4288..6201
                     /gene="mtlA"
                     /locus_tag="QIE41_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDS3530624.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            6430..7578
                     /gene="mtlD"
                     /locus_tag="QIE41_001730"
     CDS             6430..7578
                     /gene="mtlD"
                     /locus_tag="QIE41_001730"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDS3530625.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            7578..8165
                     /gene="mtlR"
                     /locus_tag="QIE41_001731"
     CDS             7578..8165
                     /gene="mtlR"
                     /locus_tag="QIE41_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDS3530626.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(8177..8386)
                     /locus_tag="QIE41_001732"
     CDS             complement(8177..8386)
                     /locus_tag="QIE41_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530627.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            8670..9032
                     /locus_tag="QIE41_001733"
     CDS             8670..9032
                     /locus_tag="QIE41_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDS3530628.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            9593..10276
                     /locus_tag="QIE41_001734"
     CDS             9593..10276
                     /locus_tag="QIE41_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDS3530629.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHLEKQQTTVINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTMNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQPA
                     ETRQRKNKK"
     gene            10320..15551
                     /locus_tag="QIE41_001735"
     CDS             10320..15551
                     /locus_tag="QIE41_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDS3530630.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGVQAQAGRDNGQGVNYGQGTGTGWVAIGEDAKANSFTDTGGGSSTAVGYHSTA
                     DGRWSTALGAKTHSLGEASVALGINTTSAGERSLAIGASATSTGGFSIALGRYANSVG
                     EFSIAQGDHAETGADDAIAFGRESKALGIMSIALGATANASKEYAMALGASSAASAAN
                     AIAVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIA
                     LGSGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALG
                     QGSYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDI
                     STTSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGL
                     DNNTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNT
                     ANITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNVKDGDLTAGSTD
                     AVNGSQLKTTNDNVATNTTNITNLTDSVGDLKDDALLWNGTAFSAAHGTDATSKITNV
                     KDGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNA
                     TAGAFSAAHGTDATSKITNVKDGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNTTN
                     ITNLTDAVDSLGDDSLLWNATAGAFSAAHGTEATSKITNVKDGDLTAGSTDAVNGSQL
                     KTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNATAGAFSAKHGTNGTDSKI
                     TNLLAGTVSSDSTDAINGSQLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNN
                     VGDALAAINTSFSTSLGDALLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTS
                     SDAINGSQLYDTSKYIADTLGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTL
                     DDALLWDATANGGNGAFSAGRGVDKTASVITNVADGAISSTSSDAINGSQLYTTNKYI
                     ADALGGDAEVNADGSITAPSYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNA
                     SHDGKASIITNVADGNIGEGSTDAINGSQLFNTNMLIQQNSEIINQLAGNTSETYIED
                     NGAGINYVRTNDNGLAFNDASASGIGATAVGYNAVASGESSVAIGQGSSSTVDTGIAL
                     GSSSVSSRVIVKGSRDTSVSEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSE
                     AHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGEDSLAMGAKTIVNSNAGIGI
                     GYGAYVDANALNGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGE
                     FSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRV
                     TNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVA
                     NEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNI
                     TLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESK
                     LSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGS
                     TNSQGEYSAALGAGIQW"
     gene            15849..17504
                     /gene="lldP"
                     /locus_tag="QIE41_001736"
     CDS             15849..17504
                     /gene="lldP"
                     /locus_tag="QIE41_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDS3530631.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYTAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            17504..18280
                     /gene="lldR"
                     /locus_tag="QIE41_001737"
     CDS             17504..18280
                     /gene="lldR"
                     /locus_tag="QIE41_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDS3530632.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            18277..19467
                     /gene="lldD"
                     /locus_tag="QIE41_001738"
     CDS             18277..19467
                     /gene="lldD"
                     /locus_tag="QIE41_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDS3530633.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQELGKELPAALAPIAK
                     GNAA"
     gene            19515..19988
                     /gene="trmL"
                     /locus_tag="QIE41_001739"
     CDS             19515..19988
                     /gene="trmL"
                     /locus_tag="QIE41_001739"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDS3530634.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(20041..20862)
                     /gene="cysE"
                     /locus_tag="QIE41_001740"
     CDS             complement(20041..20862)
                     /gene="cysE"
                     /locus_tag="QIE41_001740"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDS3530635.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(20941..21960)
                     /gene="gpsA"
                     /locus_tag="QIE41_001741"
     CDS             complement(20941..21960)
                     /gene="gpsA"
                     /locus_tag="QIE41_001741"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDS3530636.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(21960..22427)
                     /gene="secB"
                     /locus_tag="QIE41_001742"
     CDS             complement(21960..22427)
                     /gene="secB"
                     /locus_tag="QIE41_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDS3530637.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(22490..22741)
                     /gene="grxC"
                     /locus_tag="QIE41_001743"
     CDS             complement(22490..22741)
                     /gene="grxC"
                     /locus_tag="QIE41_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDS3530638.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(22883..23314)
                     /locus_tag="QIE41_001744"
     CDS             complement(22883..23314)
                     /locus_tag="QIE41_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDS3530639.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            23559..25103
                     /gene="gpmM"
                     /locus_tag="QIE41_001745"
                     /gene_synonym="pgmI"
     CDS             23559..25103
                     /gene="gpmM"
                     /locus_tag="QIE41_001745"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDS3530640.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            25113..26396
                     /gene="envC"
                     /locus_tag="QIE41_001746"
     CDS             25113..26396
                     /gene="envC"
                     /locus_tag="QIE41_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDS3530641.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRSSLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            26400..27359
                     /locus_tag="QIE41_001747"
     CDS             26400..27359
                     /locus_tag="QIE41_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDS3530642.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDMSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(27346..28380)
                     /gene="waaH"
                     /locus_tag="QIE41_001748"
     CDS             complement(27346..28380)
                     /gene="waaH"
                     /locus_tag="QIE41_001748"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDS3530643.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEAATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(28619..29644)
                     /gene="tdh"
                     /locus_tag="QIE41_001749"
     CDS             complement(28619..29644)
                     /gene="tdh"
                     /locus_tag="QIE41_001749"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDS3530644.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(29654..30850)
                     /gene="kbl"
                     /locus_tag="QIE41_001750"
     CDS             complement(29654..30850)
                     /gene="kbl"
                     /locus_tag="QIE41_001750"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDS3530645.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            31064..31996
                     /gene="rfaD"
                     /locus_tag="QIE41_001751"
     CDS             31064..31996
                     /gene="rfaD"
                     /locus_tag="QIE41_001751"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDS3530646.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            32006..33052
                     /gene="rfaF"
                     /locus_tag="QIE41_001752"
     CDS             32006..33052
                     /gene="rfaF"
                     /locus_tag="QIE41_001752"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDS3530647.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            33056..34015
                     /gene="rfaC"
                     /locus_tag="QIE41_001753"
     CDS             33056..34015
                     /gene="rfaC"
                     /locus_tag="QIE41_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDS3530648.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTVREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVVCRAPLMNLNNLEAAAVYKKITLI"
     gene            complement(34063..>35037)
                     /locus_tag="QIE41_001754"
     CDS             complement(34063..>35037)
                     /locus_tag="QIE41_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDS3530649.1"
                     /translation="RGYLESGKLLVFSSLTFLLLSKNKFRQNEKLHFISAILVQIIML
                     CRAYYQGIYLHADRIPLSAMNGNIGQMGAATIAAYVITFTTLYSSIVFIKMQSKYKWF
                     LFYANFALSFAAVMMTGTRAAIFTFPLMIMVILFLQHRDQKVFLFKGLSGVFILLLAC
                     GLIFNKEIDRRINSLKADVISYATKNNSQSSVGARFAMVNAGIKGSPDGFNWQSLEQR
                     AEKIKALSAENNIYSGALLFLDVHMHNEIVESLSTKGKIGLLVLIMFYVAMIYYCIRE
                     KKYILLVFPASIMLFGISDVITHAKPIPASWIVCLFLSISFLSKNEPQ"
BASE COUNT         9132 a         8788 c         8783 g         8334 t
ORIGIN      
        1 atccggtatc tggtttcgat ccattagggt taaaagtgtc ttttcaggga gatgaaagta
       61 cacaaaaaac tttaaaagaa gcatataaag ctgtagcaga aacaaagttt ggacataaaa
      121 taacagaaga attagagtca tcagagcatg aatatatatt tagaggatta agaaaaggca
      181 taaaccaaac atgctacgat gatactgaat atagttttta cattgatatt gataacgacc
      241 atagctcatg tgtatatcaa ggaaaaaaca aagcatgtgc catgaaaccc actctgttga
      301 gtgtggttct tgcgcatgaa atggggcatg caaagggaat gaaagacgat ggaactgact
      361 cgatggctaa tgttgataaa tatgaaaacc catttcgtaa agagttgggc ttgccagcaa
      421 gaatgaaata ttaaacattt ttatattgct tttgggtatg tttttaatat atcaccagtt
      481 taggagatgg tatgaaagta tgtctgtatt ttatttccgt atttttttca tcttgctgta
      541 tagctgatgt tttccattat aggatgcctt ttcgcatcca aacatattcc cctgtcacta
      601 aaagtaaaat tgaaaatttt gcaataaaag atggtgcaca aaaattatta cagataaatc
      661 gtgagaagtt attaatgtta ctgaacaggt ctagtaatga gtgttaccat tttgatgata
      721 aaaatgttag gatttatatt attgatggta aagacagata ttatattgat aaaaacgggg
      781 tggtgtcata tggcgttcaa gataatgtca tatgctctaa taataaaatt aatgaagatt
      841 ccatagaaaa aattttatta gaagggaata ttgacttttg gaaaaaactg aaaattaagt
      901 gaaatgaacg aaagagaaaa ttaacaatga gcggaaaacc agcggtatac taaggcgatg
      961 ccatgttgaa agatagtcca atagtctaaa gtggagaact caatgaagca aaccggacga
     1021 ggcttgctta acagtcctgg agcaaccaat agaaaaccta atggtatacg tttagtaccg
     1081 tcagcaaata cagaaacgta taatcgtgat ggatttttaa cacatagttg tttaaacgct
     1141 tttggtccat ctttaggacc tcgattctgt tccgagggtt gtataactgg ttcatctaat
     1201 gacatgcaaa aattaaatga gctaatattt tcagagccag atagcacatt aacagtgacg
     1261 gactagaaat atgacaaggt tattaacaat tgtgcttttc ttctctattt ctgcgaatgc
     1321 agttactgta aaccaatttc agagagagtt ggatttggca gacaaggcaa tattatgggc
     1381 actaatatca gccagtaccc aagagggacg aaaagcgtgt tcacttagct atttttcatg
     1441 taaagccgca gaggctgaac tcggactggc atatatggct gctaatgata acaaggcatt
     1501 tctcacatcc ttatctagaa taatgatgta caaaatagat gctggacttt ccgaatccta
     1561 tacatgctat ttattaagca agggaaagat cataagacca tatctgaaga atctaaatcc
     1621 acaccaactt gtcgctgatt gcattgaaac tgtaaacaaa ataaaagata aaaataaaaa
     1681 aatcattgat atagattcag ttaatatttg caatgataat aaaaatataa attggagagt
     1741 taatagtact attgtagcca ttgatgattc tataaaatgc atcgatgaat aataacttaa
     1801 ttatatttaa aaacatatta ataactcact ctccaggagt ttttttaact tagtgttata
     1861 atgaatacga taattaaaag tgaagagaaa aatagttgta tacatatatt acatgaacct
     1921 acattgtgat ctataaatta aatattttta aatgtgactg tcatccttca gataataaag
     1981 ccatcatcgc aatagatgga aaagcactcc tgctcttttt tgattagagc cgtggcagag
     2041 gaacaactca tatcatcatt gcgatctcga caatgtatag tctgatcctt cggcagatca
     2101 agacggataa aaaatccaat gagttcacac tcatcccgaa cttcttaaca taatggatat
     2161 taacgaaaaa tcatcacagc cgatgaaata atatgcaaaa ataattagtc cttgcaggga
     2221 gcaaagttcc ataatctccc ccgcccccct caatgatcca aataaagata atgcatccag
     2281 ctggtcattc ttagcagcac tttccgcatc accgaaacat ggctgaaatg gtcggagtaa
     2341 accgccacct gggcgtagat gccgtcgggt aaggcatcga tatcatcgtt agggtccagt
     2401 tcaatggttc ccagcacacc gtccgtgccg ggcacgaccg ttaatgattg caataccccc
     2461 tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatgc tagtcagttt gccgtggaac
     2521 acctgcccag gtagcgcgtt aaacaccact tccgcatcat cgccgggttt cagacgtaac
     2581 agcgagtttt gccgaaattg ggcgacaatt tgccgttttt gctcggggat gaaaaccatc
     2641 accggacgca acggcaaggc agctgcgtat gtacctgggc ggatcagtac ctgagtaacg
     2701 tagccattgc tcggcgcgcg aatgacagtc tgctcaaggt tatattttgc ttcagtaagt
     2761 tgcgctctta agctcacaat ctgcgattgc tcgccgttaa ccatactgtc gagctggctc
     2821 tggatctgcg cctgctccgc caccgagcct ttcaccagcg cgtcctgcgc gaggaagttt
     2881 tgccgcgcat cgtcgatgtc acgttccgag aacggattca ccgccgcctg gctgcctttc
     2941 agataacgtt gataattttt aaacagacgg tcgcgctccg ctgaaacctg ggtggtgttg
     3001 gcctgcgctt cagagagctg cgcgcgcagc gtctttatat tatgcgtcgc cgtcatcagg
     3061 tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg ggtcgagttt aaaaagcacc
     3121 tcgccctttt gaataagctg attattcttg tcagtgactt cagtaacaat tcccgtcacc
     3181 tgtggcgtga tggggatcgc tatcactgcc ttttgcgcgg taaaagtgta agggtggttg
     3241 tagttcatca acaaaatcaa accactcacc agaaacacgc ctcccagcgc cgccgtcgcc
     3301 agcgtccact gattcaccgg aatgcggaag attttaaaga ccgcccacgc cagcgccacg
     3361 taagttaaaa caatcaatag atccatattt agatctccgg gaacgtggaa tagtcagtct
     3421 ttttctcggc ggcgagttga tgctccagcc gggaaattcg gtcagaaagt gcggcgattt
     3481 ccgggtcagt cgctttctct tgcgacgcaa catgtgactg catgccccag ccgcgctccg
     3541 gttgatagag cgtcgcccag atccacagga acggccagat aacatgtaga gtaaacaggc
     3601 tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt ttggcaatca
     3661 gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag acgaagatca
     3721 gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt ttatcagtgt
     3781 tgttttttgg ctggcagcca gaagggagtc aggctgatat tttgacaata atccgggttc
     3841 gcgattctcg ccataacacc aaagaataat tttaagaggt gatgagttgc ttagttacat
     3901 aacgatttca tgacgaaggc ataacatgct gtagatcaca tcaggtgaac gccgtaagaa
     3961 aatatcttgt gattcagatc acaaagattc aacaaaccat caaaataaaa atgtgacatc
     4021 actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt ttacacaaga
     4081 gttttgtgat gagcatcacg tcaattacct ctctaccccc tatatttatg tgactgatat
     4141 cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc gcagtatcta
     4201 caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc agcctgcgcg
     4261 acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa agtgcaaagc
     4321 tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat cgcgtggggt
     4381 atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct ggcgaagctg
     4441 gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg tggtaagctg
     4501 gtaggcggcg aacgtggcgg cgtagtcggt gccattacca ccatgggcgt tatcgtcggc
     4561 gcagacatgc cgatgttcct aggttctatg attgcaggtc cgctgggcgg ctggtgcatt
     4621 aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat gctggtgaat
     4681 aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct cggcattggc
     4741 ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat ggttgtccat
     4801 gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt cctcaacaac
     4861 gccattaacc acggtatctt ctcgccgctg ggcattcagc agtcccatga actgggtaaa
     4921 tccatcttct tcctgattga agctaaccca ggtccgggta tgggcgtgct gctggcgtac
     4981 atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat catccacttc
     5041 ctgggcggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg tctgattctg
     5101 gccgttatcc tcggtggtat gactggcgtg ttcacgctga ctatcctggg cggtggtctg
     5161 gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc aaaaggtgca
     5221 tacttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt tgtctctgcc
     5281 attctgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc aactcgtcgt
     5341 atgcaggaca tgaaagctga gtctaaaggc gcgtctccgc tgtctgcggg cgatgtgact
     5401 aacgacctga gccacgtacg taaaatcatc gttgcctgtg atgccggtat gggttccagt
     5461 gcgatgggcg caggtgtgct gcgtaagaaa attcaggatg caggtctgtc gcagatttcg
     5521 gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctggtcat cactcaccgt
     5581 gacctgaccg aacgcgctat gcgccaggtt ccgcaggcac agcatatttc gctgaccaac
     5641 ttcctcgaca gcggcctgta caccagcctg accgaacgtc tggttgctgc ccagcgccat
     5701 actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgatga ttccagtgct
     5761 aacctgttca agctaggcgc ggagaacatc ttcctcggtc gtaaagcggc caccaaagaa
     5821 gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga gccggaatac
     5881 gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga gtccatcgcg
     5941 gtaccgcacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt ggtgttctgc
     6001 cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg tctggtgatt
     6061 ggtattgctg cccgtaacaa cgagcacatt caggttatca ccagcctgac caatgcactg
     6121 gatgatgagt ctgtcatcga gcgtctggca cacaccacca gcgtggatga agtgctggaa
     6181 ctgctggcag gtcgtaagta atccaatccc acctctccac atggagaagg tggggcttat
     6241 tgcctgatgc gctacgctta tcaggcctac aaaacaaagc gtagtttgtt gaatttgcac
     6301 gttcttgtag gccggataag gcgtttacgc cgcatccggc gcggcccctc tccacatgga
     6361 gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag
     6421 gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt
     6481 aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt
     6541 gatgccctga atgcccgtca tagctatcag gttcatgtgg tcggtgaaac cgagcaggta
     6601 gataccgttt ccggcgtcaa tgccgtcagc agcattggtg atgatgtcgt tgatctgatt
     6661 gctcaggttg atttagtcac taccgccgtt ggtccggttg tgctggaacg tattgccccg
     6721 gcaatcgcca aagggctggt gaaacgtaaa gaacaaggta atgaatcccc gctgaacatc
     6781 atcgcttgtg aaaacatggt acgcggcacc acgcagctga aaggccatgt gatgaacgcc
     6841 ctgccggaag atgccaaagc gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt
     6901 gaccgcatcg tgccgccttc ggcttctgca accaacgatc cgctggaagt gacggtagaa
     6961 acattcagcg aatggattgt tgataaaacg cagttcaaag gcgcgctgcc gaacatccca
     7021 ggcatggagt taaccgacaa cctaatggca tttgtcgaac gtaaactctt caccctgaac
     7081 acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgat
     7141 gcgattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagcggtgcg
     7201 gtactgatca agcgctacgg ctttgacgcg gacaaacatg cggcgtacat ccagaaaatc
     7261 ctcggtcgtt tcgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg
     7321 ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac tgctcggaac gctggaatat
     7381 ggtctgccac ataaaaacct gattgaaggt attgccgctg caatgcactt ccgcagtgaa
     7441 gatgatccgc aggctcagga actggcagcg ctgattgccg acaaaggccc acaagccgcg
     7501 ctggcacaga tttccggtct tgatgccaac agcgaggttg tatccgaggc agtaaccgct
     7561 tataaagcaa tgcaataatg gtggaccagg cgcaggatac cctgcgcccg aataacagat
     7621 tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc
     7681 gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg
     7741 aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg
     7801 ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac
     7861 tcatttacgg gttgggtgtc attaaccgcc aggaatacga agatgcggaa ctgctgatgg
     7921 cattgcgtga agagctaaat cacgacggca acgagtacgc gtttaccgac gacgaaatcc
     7981 ttggaccctt tggtgaactg cattgcgtgg cggcgttacc accgccgcca cagtttgaac
     8041 ctgcagactc cagtttgtat gcaatgcaaa ttcagcgtta ccaacaggct gtgcgatcaa
     8101 caatggtcct ttcactgaca gagctgattt ccaaaatcag cttaaaaaaa gccttccaaa
     8161 agtaagcaac gcctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat
     8221 actccagacg ttgtagatat aacggcacgc tttcatcagg tattccggac ggaatcgcgt
     8281 tacggggagg tagttttttc agatactccc ggaacgcctg gctcgatgcc atgaaatcta
     8341 cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt
     8401 attaccccgc gccgggaatg cgcggccgcc aattttagtt tagatcccgg tataaatgac
     8461 attaaggaaa acgtgctcaa tcctcaataa tcatccgctt ttgttaacta tcttttttct
     8521 ttaaatttct taattaatca ataaattgcg tagtttttaa gcatgttaat gtttacgtta
     8581 tagaactatt gcaatgtcat cagttgtgaa caaagcacct ggtcgcgcat actaggggct
     8641 ataaatttat ctttatcaga agccatcaca tgaaagaagt cgaaaaaaac gaaatcaaac
     8701 gtctcagcga tcgtctggac gccatccgcc accagcaggc cgatctgtcg ctggttgaag
     8761 ccgcagacaa atatgccgaa ctggaaaaag agaaagccac tctggaagca gaaattgctc
     8821 gcctgcgcga agttcacagc cagaaactca gtaaggaagc acagaagctg atgaagatgc
     8881 cgttccagcg cgcgattact aaaaaagagc aagctgatat gggcaaactg aagaagagcg
     8941 ttcgcggact ggtcgttgtg cacccaatga ccgcgctggg ccgcgaaatg ggcctacaag
     9001 agatgacagg gttctctaag actgcgtttt aagttccttt ctcgtcgctg taattacagc
     9061 cactcacgat ttactcgctc ctgctggcac atgcaggagc ctgttgtaga gatcgaatat
     9121 ttataacgtt cttaacatta tatatttaac tccacctaca cttctgagcg cccggagcag
     9181 gctaaattct gaaaatcagg tacaaattaa agaatgatgt atcaatatta acaatgaatt
     9241 gccgcatatc aatgcttgaa acataattaa ttatttaaat ttttctatca gcatcgcaaa
     9301 attcgcaata aaacatcact caactgaata tttatctttc cctgccccgg gcctttatcc
     9361 ggtgaaacat ttgacattga ttgacaccga tcattaaaat ccgcgcgatc aatttattta
     9421 ttggctacaa actaaaattg tttgacactt aaaaacatat tcgcacaacc cattaataca
     9481 aaaattctct aacaaattta tttggcggat atgccccact ctaatcattc ataaaaaaaa
     9541 tgattgtact gtaattatca acgtatcggg attatgaaaa ggaattcatc ctatgaacct
     9601 aaagaaaaca ctgttaagcg tgttaataat agtgcctctc tgcttattgg cagggtgtga
     9661 ctatattgaa aaagcgagta aggtcgacga cctcgttaca cagcaagagt tgcaaaaaag
     9721 caaaattgag gcgcttgaga aacagcagga actcgataag cgaaagatag aacatcttga
     9781 aaaacaacaa actactgtca taaatagtac caagacgctc gctggcgtgg tgaaagcggt
     9841 taaagacaag caggacgaat ttgtctttac agaatttaac ccagcacaaa cacaatattt
     9901 cattttaaat aacggatcgg tcggtttggc aggccgggtg ctggctattg atgcggttga
     9961 aaatggcagt gttattcgta tttcgctggt taacctttta agcgttcccg tatcaaatat
    10021 tggctttcat gcgacatggg gcaatgaaag acccaccgat gccaaagctc tggcaaagtg
    10081 gcaacaatta cttttcaaca ccaccatgaa ttccactctg caattaatgc ctggtcaatg
    10141 gcaagacatt aatctgacat taaaaggcgt ttcgcctaat aacctgaagt atctgaagtt
    10201 gtccatcaat atggccaatc tccagttcga cactgttcaa cctgctgaaa ctcggcagcg
    10261 aaaaaacaaa aaataaatgc agcttctgcg cttatatcaa ggaatagaga gcatcaataa
    10321 tgaacaaaat ttttaaagtt atctggaatc cggcaacagg cagttacacc gttgccagtg
    10381 aaacggcaaa aagccgtggc aagaaatccg ggcgcagtaa gctgttaatt tctgccctgg
    10441 ttgctggggg aatgttgtcg tcgtttgggg tacaggctca ggcaggaagg gacaacggtc
    10501 agggcgtcaa ttatggccag ggaacaggaa caggctgggt tgctatcggt gaagatgcaa
    10561 aagcaaatag ttttacagac accgggggtg gctccagcac cgcagtaggt tatcactcaa
    10621 ctgctgacgg aaggtggtca acagccctcg gtgctaagac ccattcgctt ggcgaagcat
    10681 cagtggcgtt agggattaac actacctcag caggtgagcg aagcctcgct ataggtgcat
    10741 cggcaacgtc aacgggtgga ttttcaatcg cacttggtcg ttatgcgaac tcagtaggtg
    10801 aattttctat tgcacaaggg gatcacgcag agactggtgc tgatgacgcc atcgcatttg
    10861 gacgtgaaag caaggcctta ggaattatga gtatcgccct cggagccacg gctaatgcgt
    10921 cgaaagaata tgcaatggcg ctgggtgcaa gtagcgccgc atctgcagct aacgcgattg
    10981 cagtaggccg caacagtgct gcagctggcg tagacagcct cgcattcggt agactatccg
    11041 ccgccaacgc cgcaaacgcg attgctatgg gtgcagagag caaagctgct gaaaatgcga
    11101 cggcagttgg tactaacgcg gaggcaaatg gacttaatag tatcgcgttg ggctccggca
    11161 gtattgcaga tgtcgacaac acgattgctc tgggcaatca aagtcaggca gtagcggctg
    11221 gcgcaattgc catcggtcaa gggaataaag ctgacggcgc aaatgctatc gcgctgggta
    11281 atggtagtat tacaggtggt gtaaatgcta ttgctcttgg acaaggcagt tatgccggtt
    11341 tagaaaatgg cactgcaatt ggtgctcaag ccagtgctca agggaaaaat tcagttgctc
    11401 tgggtgctgg ttctgtagcg actgacgcgg atactgtttc tgtgggtaac acaacagctc
    11461 agcgacaaat tgtcaatatg gcagcaggtg atatcagcac taccagtact gatgccatca
    11521 atggatcaca gctttatgct atcagtaagt cagtagcgga caatcttggt gggggggcta
    11581 ccgtcaatgc gcaaggcgtc gttacttccc caaattacag gctgaaaagt ggtattttcg
    11641 gcactgttgg cgacgcctta acgggcctgg acaataatac gttacaatgg gactccctta
    11701 aaaaggcata tagtgcggca catggtacag atactaccag taccatcacc aacgttaaag
    11761 acggcgctat ttctgatacc agtaaggatg cggttaacgg ttctcagctg aaaaccacca
    11821 acgataacgt agcgaccaat actgccaata tcaccaccaa cactaacagt atcaataccc
    11881 tgacggattc cgttggcgac cttaaagacg atgccctgct gtggaatggc accgcgttca
    11941 gcgccgcgca cggcaccgaa gccaccagca aaatcaccaa cgtcaaagac ggtgacctga
    12001 cggctggcag caccgacgcg gttaacggct ctcagctcaa aactactaac gataatgtgg
    12061 caaccaacac caccaacatc accaacctga cggattccgt tggcgacctt aaagacgatg
    12121 ccctgctgtg gaatggcacc gcgttcagcg ccgcgcacgg taccgatgcc accagcaaaa
    12181 tcaccaacgt caaagacggt gacctgacgg ctggtagcac tgacgcggtt aacggctctc
    12241 agctcaaaac cactaacgat gccgtggcag ccaacaccac caatatcgcc acgaacacca
    12301 ctaacatcac caacctgact gacgctgttg acagtctcgg tgatgactct ctgctgtgga
    12361 acgctactgc gggggcattc agcgccgcac acggtactga tgccaccagc aaaatcacta
    12421 acgtcaaaga cggtgacctg acggctggca gcactgacgc ggttaacggc tctcagctca
    12481 aaaccactaa cgatgccgtg gcagccaaca ccaccaatat cgccacgaac accactaaca
    12541 tcaccaacct gactgacgct gttgacagcc tcggtgacga ttccctgctg tggaacgcta
    12601 cggcgggggc attcagcgcc gcgcacggta ccgaagccac cagcaaaatc accaacgtca
    12661 aagacggtga cctgacggct ggcagcactg acgcggtaaa cggctctcag ctgaaaacca
    12721 ctaacgatgc cgtggcagcc aacaccacca atatcgccac gaataccacc aacatcacca
    12781 acctgactga cgctgttgac agtctcggtg atgactctct gctgtggaac gctacggcgg
    12841 gtgcattcag tgccaaacac ggtaccaacg gtactgatag caaaatcacc aacttactgg
    12901 caggcacggt atcctctgat agcactgacg ccattaatgg ctcacaactt tatggcttag
    12961 ctgattcatt tacgtcatac cttggcggtg gtgctgatat cagtgatgcg ggtgtattaa
    13021 ccgggccaac ctacactatt ggtggtactg actacaataa cgtcggtgat gctctggctg
    13081 ccattaacac gtcatttagc acatcactcg gtgacgccct actctgggat gcgaccgcca
    13141 agggcggtga cggtgcgttt agcgccggtc gcggaaaaga taacacggcc agcatcatta
    13201 ctaatgttgc agacggtgca atttcctcta ccagcagcga cgctattaac ggctcacagc
    13261 tctatgacac cagtaagtac attgccgata ctctgggtgg tgacgcagaa gtcaatgctg
    13321 acggcacaat caccgcaccg acttatgcca tcgctggcgg cagttacagc aacgtcggtg
    13381 acgcgctgga agcgatcgat accacgctgg atgacgctct gttgtgggat gcaacagcca
    13441 atggcggtaa tggtgcattt agcgccggtc gcggagtaga taaaacggcc agcgtcatta
    13501 ctaacgtcgc agacggtgca atttcctcca ccagcagcga tgctattaac ggctcacaac
    13561 tgtataccac caacaagtac atcgctgatg cgctgggtgg tgacgcagaa gtcaacgctg
    13621 atggttctat tactgcgccg agttacacca ttgcaaatgc cgagtacaac aacgtcggtg
    13681 acgccctgga tgcgctcgac gataacgctc tgctgtggga tgcaacggcc aatgacggcg
    13741 caggtgccta caacgccagc catgatggca aggccagcat catcacaaac gttgctgatg
    13801 gtaacattgg cgaaggcagc actgacgcca tcaacggttc tcagctgttt aacaccaata
    13861 tgctgatcca gcagaacagc gaaatcatca atcagcttgc cggtaacacc agcgaaacct
    13921 acatcgaaga taacggtgcg ggcattaact atgtacgtac caacgacaac ggcttagcgt
    13981 tcaacgatgc cagcgcttca ggtattggcg ctacggctgt gggttataac gctgtcgcct
    14041 caggcgaaag cagcgtagcc attggtcaag gtagcagcag caccgttgat accggtattg
    14101 ccctgggtag cagttccgtt tccagccgtg taatagttaa aggttctcgt gacaccagcg
    14161 tgtcggaaga aggtgttgtg attggttatg acaccacgga tggcgaacta cttggtgcgt
    14221 tgtctattgg tgatgacggt aaatatcgtc aaatcatcaa cgtagccgat ggttccgaag
    14281 cccatgacgc cgttacggtt cgccagttgc aaaatgccat tggtgcagtc gctaccacgc
    14341 cgaccaaata cttccacgcc aactcaacgg aagaagattc actggcagta ggtgaagact
    14401 cgctagcaat gggcgcgaaa actatcgtta atagtaatgc gggtattggt atcggttatg
    14461 gtgcctacgt ggacgcgaat gcacttaatg gcattgctat cggtagcaac gcgcgtgcaa
    14521 accatgcaaa cagcattgct atgggtaatg gctcacagac gactcgtggt gctcaaactg
    14581 gctacaccgc ctacaacatg gacgcaccac agaactctgt gggtgagttc tctgtcggca
    14641 gtgaagacgg tcaacgtcag atcaccaacg tcgcggctgg ttcggctgat accgatgcgg
    14701 ttaacgtggg tcagttgaaa gtaacggacg cgcaggtttc ccagaatacc cagagcatta
    14761 ctaacctgaa caatcaggtg acgaatctgg atactcgcgt taccaatatc gaaaacggca
    14821 ttggcgatat cgtaaccacc ggtagcacca aatacttcaa gaccaacacc gatggcgtag
    14881 atgccaacgc gcagggtaaa gacagtgttg caattggttc tggttccatt gctgccgctg
    14941 acaacagcgt cgcgctgggt accggttccg tggccaacga agaaaacacc atctctgtgg
    15001 gttcttctac caaccagcgt cgtatcacca acgttgctgc aggtgttaat gccaccgatg
    15061 cggttaacgt ttcgcagctg aagtcttctg aggcaggcgg cgttcgttac gacaccaaag
    15121 ctgatggttc tatagactac agcaacatca ctctcggtgg tggtaatggc ggtacgactc
    15181 gcatcagcaa cgtctccgct ggcgtcaaca acaacgacgc agtgaactat gcgcagttga
    15241 agcaaagcgt gcaggaaacg aagcaataca ccgatcagcg gatggttgag atggataaca
    15301 aactgtccaa aaccgaaagc aagttgagcg gtggtatcgc ttctgcaatg gcaatgaccg
    15361 gtctgccgca ggcttacact ccaggtgcca gcatggcctc tattggtggc ggtacttaca
    15421 atggtgaatc ggctgttgct ttaggtgtgt cgatggtgag cgccaatggt cgttgggtct
    15481 acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcgctc ggtgccggta
    15541 ttcagtggta atcatccatt aacttagtgt gactaagggc aattctctga tgaggattgc
    15601 ccttttcttt accagacatc tccctccacc aagaattggc cctaccaatt attcgcttat
    15661 ctgacctctg gttcacaatt ccccaattaa aactcacatc aatgttgcca atgcataaca
    15721 tttagttaac cattcattgt cattatccct acacaacaca attggcagtg ccacttttac
    15781 acaacgtgtg acaaggagat gagcatcaga ctcattacac gatgtgcgtg gactccagga
    15841 gacctgcaat gaatctctgg caacaaaact acgatcccgc cgggaatatc tggctttcca
    15901 gcctgatagc atcgcttccc atcctgtttt tcttctttgc gctgattaag ctcaaactca
    15961 aaggttatgt cgccgcctca tggacggtgg caatcgccct tgccgtggct ttgctgttct
    16021 ataaaatgcc ggtcgctaac gcgctggcct cggtgattta tggcttcttc tacggtttgt
    16081 ggcccatcgc gtggatcatt attgcggcag tgttcgtcta taagatctcg gtgaaaaccg
    16141 ggcagtttga catcatccgc tcgtctattc tttcgataac ccctgaccag cgcctgcaaa
    16201 tgctgatcgt cggtttctgt ttcggcgctt tccttgaggg agccgcaggc tttggcgctc
    16261 cggtagcaat taccgccgca ttactggtcg gtctgggctt taaaccactg tacgccgccg
    16321 gactgtgcct gattgttaac accgcgccag tggcatttgg cgcgatgggt attccgattc
    16381 tggtcgccgg gcaggtaaca ggcatcgaca gctttgagat tggtcagatg gtggggcgtc
    16441 agctgccgtt tatgaccatt atcgtgctgt tctggatcat ggcgattatg gacggttggc
    16501 gcggtatcaa agagacgtgg cctgcggtcg tggttgcagg tggttcgttt gccattgctc
    16561 aatacctcag ctctaacttc attgggccgg aactgccaga cattatctct tcgctggtat
    16621 cactgctctg cctgacgctg ttcctcaaac gctggcaacc agtgcgcgta ttccgcttcg
    16681 gtgacttggg ggcgtcacag gttgatatga cgctggctca caccggttac accgcaggtc
    16741 aggtgcttcg cgcctggaca ccgttcctgt tcctgaccgc caccgtaacg ctgtggagta
    16801 ttccaccgtt taaagccctg ttcgcttcgg gtggtgcgct gtatgaatgg gtgatcaata
    16861 ttccggtgcc gtacctcgat aaactggttg cccgtatgcc gccagtggtc agcgaagcga
    16921 cagcgtatac cgccgtgttt aagttcgact ggttctctgc caccggtacc gccattcttt
    16981 ttgctgccct gctctcgatt gtctggctga agatgaaacc atctgacgct atcagcacct
    17041 tcggcagcac gctgaaagaa ctggctctgc ctatctactc catcggtatg gtgctggcgt
    17101 tcgcctttat ctcgaactat tccggactat catcaacgct ggcgctggca ctggcgcaca
    17161 ccggccatgc attcaccttc ttctcgccgt tcctcggctg gcttggtgtc ttcctgaccg
    17221 gatcggatac ctcatctaac gccctgttcg ccgcgctgca agccactgca gcacaacaaa
    17281 ttggcgtttc tgacctgttg ttggttgccg ccaacaccac cggtggtgtc accggtaaga
    17341 tgatctctcc gcaatctatc gctatcgcct gtgcggcggt aggcctggta ggcaaagagt
    17401 cagatctgtt ccgctttact gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga
    17461 tcaccacgct tcaggcttat gtcttaacgt ggatgattcc ttaatgattg ttttacccag
    17521 acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga
    17581 ggcgggcatg aagttgcccg ctgaacgtca actggcgatg caactcggcg tatcgcgtaa
    17641 ttcactgcgc gaggcgctgg caaaactggt gagcgaaggc gtgctgctta gtcgacgcgg
    17701 cggcgggacg tttattcgct ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc
    17761 gctgaaaacg ctgatggccg atgatccgga ttacagtttc gatattctgg aagcccgcta
    17821 cgccattgaa gccagcaccg catggcatgc agcaatgcgc gccacaccag gcgaaaaaga
    17881 gaaaattcag ctttgctttg aagcaacgct aagtgaagac ccggatctcg cctcacaagc
    17941 ggacgttcgt tttcatctgg cgattgccga agcctcacat aacatcgtgc tgctgcaaac
    18001 catgcgcggt ttcttcgatg tcctgcaatc ctctgtgaag catagccgcc agcggatgta
    18061 tctggtaccc ccggtttttt cacagctgac cgaacaacat caggcagtca ttgacgccat
    18121 ttttgccggt gatgctgacg gggcgcgtaa agcaatgatg gcgcacctta gctttgttca
    18181 caccaccatg aaacgattcg atgaagatca agctcgccat gcacgcatta cccgcctgcc
    18241 tggtgaccat aatgagcatt cgagggagaa aaacgcatga ttatttccgc agccagcgat
    18301 tatcgcgccg cagcacaacg cattctgccg ccgttcctgt tccactatat ggatgggggt
    18361 gcatattctg aatacacgct gcgccgcaac gtggaagatt tgtcagaagt ggcgctgcgc
    18421 cagcgtattc tgaaaaacat gtctgactta agcctggaaa cgacgctgtt taatgagaaa
    18481 ttgtcgatgc cggtggcgct ggctccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc
    18541 gaagttcagg ctgccaaagc ggcggatgcg catggcattc cgtttactct ctcgacggtt
    18601 tccgtttgcc cgattgaaga agtcgctcca gccattaaac gtccgatgtg gttccagctt
    18661 tatgtgctgc gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa agcggcaggc
    18721 tgctcgacgc tggttttcac cgtggatatg ccgacaccgg gcgcgcgtta tcgtgatgcg
    18781 cattctggta tgagcggccc aaatgcggca atgcgccgtt acttgcaggc ggtgacgcat
    18841 ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac atgatttagg taatatctcg
    18901 gcttacctcg gcaaaccgac cggactggaa gattacatcg gctggctggg gaataacttc
    18961 gatccgtcca tctcatggaa agaccttgag tggatccgcg atttctggga tggtccgatg
    19021 gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg cagtacgttt tggtgctgat
    19081 gggattgtgg tttctaacca cggtggccgc cagctggacg gtgtactctc ttccgctcgt
    19141 gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatag ccattctggc ggatagcgga
    19201 attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg
    19261 ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc taacctgcta
    19321 aatctgatcg aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa atcgatcagc
    19381 gaaattacgc aagattcgct ggtgcaagag ctgggtaaag agttgcctgc ggcactagct
    19441 ccgatagcga aagggaatgc agcttaaggg ttagacgaat atctgctatc ctgcccccgc
    19501 actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg
    19561 gcaacatcat ccgtctttgc gctaataccg gctttcgtct gcatatcatc gaaccaatgg
    19621 gatttgcctg ggacgataag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg
    19681 ccgttacgcg tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt
    19741 ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aagctatcag gatggcgatt
    19801 atctgatgtt cggcccggaa acacgcggcc tgccagcaag cattcttgat gccctgcccg
    19861 ctgaacaaaa aattcgcatt ccaatggtgc cggacagccg cagcatgaac ctgtccaatg
    19921 cggtgtcggt cgtggtgtat gaagcctggc ggcagctggg gtatccggga gcggtattga
    19981 gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacaa tcacaggacg
    20041 tcagatccca tccccatatt caaacgtatg gttaataccg ttgaaatgct gatccatgtc
    20101 cattgacggt ttatcgctgt ctggtttacc gacaatgcgt gccggaacgc cagcggcggt
    20161 ggtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcgccc
    20221 aacttcaata ttgccgagaa ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt
    20281 cggatgacgg tcgccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga
    20341 tacgtcgttt tcaatcaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcaagcat
    20401 gataccgcgg ccaatttttg ctgccgggtg aatatcaacc tggaacgtta cagaaacctg
    20461 gttttgcaga aatattgcca gtgcgcggcg gccctgattc cacagccagt gaccgatacg
    20521 ataggcctgc aaggcatgaa aacctttcag atataacaat ggagtcgagt atttatccac
    20581 cgccgggtcg cgggtacgta ctgcctgaat atcacaggcc gcagaagcga tcatttccgg
    20641 gtcggcggcg taggcctctt ccaccacttc gcggatagca atggcaggca taattggcga
    20701 tgacagcttg ttcgccagca tgtagctcag tgcactgccc aggttttcgt gcttgagtag
    20761 cgtcgcgtgg taaaaactgg ccagcattgg ctcacaatcc gccagcgttc tggcttcggc
    20821 tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg
    20881 agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag ttccctggga
    20941 ttagtggctg ctgcgctcgt ccttgcgtgc acgacctaat aatgtcaatg ctgcctcgcg
    21001 cgcgtttttt ccgcaatata atacttgata aatttcctcg gttattggca tttcaacgcc
    21061 gaagcgatgc gccagttcgc ggacttcttt cgtattgcgg tagccttcca ccacctgacc
    21121 aatcttctcc tgcgcgcttt gtacatccat gccctgaccg agcatcatgc caaaacggcg
    21181 gttacgcgac tggttgtcgg tacaggtaag caccagatcg cccagccccg ccatgcccat
    21241 aaaggtggca ggatcggccc ccagcgccgc gccaagacgc gacatttcag ccagcccacg
    21301 ggtgatcagc gccgtacgcg cattcgcacc aaaaccgata ccgtcggaca tccccgcgcc
    21361 aatggcaatg acgtttttca ccgcgccgcc aagctgcacg ccaatgaaat ccgggttgct
    21421 gtaaacgcgg aaacttttgc cgcagtgcag cagctgctgg agatcatcgg caaaggtctg
    21481 gtcggtagag gccagcgaaa tggccgtcgg taaacctgcc gccagttctt tcgcaaacgt
    21541 tgggccagag ataaccgcca gcggaatttg atcgcctaac gcctcacgcg ccacgtcctg
    21601 caacagacgc ccggtttccg cttccagccc tttggtcgcc cacaccagac gcgcatcagg
    21661 acgcatcagc ggtttaatct gacgcagcac ttcaccaaag acatggctgg gtacgacgac
    21721 gagaatatta cggctggctg ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc
    21781 gggaaaaggc acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat
    21841 atgttcaggg tcatggcccc agaggacaac ctcgtggcca tttcttgcca gggtgatggc
    21901 aagagcggtg ccgtacgagc cggcaccgat cacagtcatt gaagcattac gttggttcat
    21961 caggcatcct gatgttcttc agtaccttcg ccagcctgct gctgcaaata gttcatgaac
    22021 agcgcatcga agttaaccgg cgcaaggttc agttgcggga atgtaccgcg ggataccatg
    22081 ctggtgatgc actcacgagc atacgggaac agaatgttcg ggcagtatgc acccaggcaa
    22141 tgtgccatct gggtgccttc gatacccgcg atggagaaaa taccgccctg ctgaacttca
    22201 cacaggaacg cggtttcttc gcccagggag gccgttacgg taacacgcag taccacttcg
    22261 taaacgtcat ctgccagttg agaagatgcc gtatccagat caagtttaac ttctggctgc
    22321 caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga aagaaatatc cttggtataa
    22381 atacgttgga tctggaaagt catttcggtg ttgttttgtt ctgacatgtg tagaaaaccc
    22441 ttaagtgttg tccttaaata ctgcgcaatg taatgccaac gttcacacgt tatttcagca
    22501 ggggatccag tccaccacgt gcatccaatg catacaagtc atcacagccg ccaatgtgct
    22561 gtgcgtcaat aaaaatctgg ggaaccgtgg tgcgaccgct gcgtttgatc atctcttcac
    22621 gcttagcggc gttgccatcg atcggcaact cctgaaaact cacgcccttg ctgctcagca
    22681 gtgctttcgc acgatggcaa tacgggcagg tttctttggt atagatttca acattggcca
    22741 taacttagct cctgtttttt taccctgaaa tttcatgttg cagggcggat gcaaaatgga
    22801 gtgtccccag aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc
    22861 atcatgaaag acgacaggta aattatttgc cgcgcaccag aggtaagttt tcgccagccc
    22921 agccagcaac gccctctttc agcacgaata cttgcgcaaa accagctttc gtcagcgcgt
    22981 ttgcaggctc ctggcactgc atgccagaac cgtctaccac gataaccggt ttgtctttgt
    23041 gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacagg ttgatagaac
    23101 ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcatctt
    23161 ctttgttgat cagacgcgta gcttcaccac gagtaatcac cttcactttc gaggtcaggc
    23221 tcttaaacgt agtcacaata accgccacca gtaacgcgat ccaggcgata ctcagtatgg
    23281 gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg
    23341 attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta
    23401 actggaatgc aattttgcgg ggcgcgacga aaaaaaagcg caaatctggc atcggcttgc
    23461 cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa
    23521 ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta
    23581 tggtactggt gattctggat ggctatggct atcgtgaaga acagcaggat aacgccattt
    23641 ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg
    23701 acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggtc
    23761 acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa
    23821 tcaaagatcg cgctttcttc gctaacccgg tactgactgg cgcggtagat aaagcgaaaa
    23881 acgcaggcaa agcagttcac attatgggtc tgctgtccgc aggcggcgta cacagccacg
    23941 aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc
    24001 tgcacgcatt ccttgacggt cgcgacactc cgccgcgcag tgctgaatcc tcgctgaaaa
    24061 aattcgaaga gaaatttgcc gcgctgggta aaggccgcgt agcgtccatc attggtcgtt
    24121 actacgccat ggaccgcgat aaccgttggg atcgcgttga aaaagcttat gacctgctaa
    24181 ctctggcgca gggtgagttc caggccgata ccgccgttgc tggtttgcag gctgcttatg
    24241 ctcgcgacga aaacgacgaa ttcgtgaaag cgaccgttat ccgtgcagaa ggtcagccag
    24301 acgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcac
    24361 gtgaaatcac tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg
    24421 ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt
    24481 acccaccagc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc
    24541 agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg
    24601 tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct
    24661 acgatctgca accggaaatg agctccgcag agctgaccga aaaactggtt gcggcaatca
    24721 agagtggcaa atacgacacc attatctgta actatccgaa cggcgacatg gtaggtcaca
    24781 ccggggtgat ggaagcggcg gttaaagcgg ttgaagcgct ggatcactgc gtggaagaag
    24841 tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg
    24901 ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag
    24961 ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgaaggcggc aaactttctg
    25021 acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta
    25081 agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga
    25141 cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta
    25201 gcgctggcgt attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat
    25261 ctattcaggc agatatcgcc gcgaaagagc gcgcggtacg ccaaaagcaa caacaacgct
    25321 ccagcctgct tgctcagttg aaaaagcagg aagaagcgat ctcggaagcc acccgtaagc
    25381 tgcgcgaaac gcaaaacacg ctcaatcaac tgaataaaca gattgatgag atgaacgcgt
    25441 cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc gcacaactgg
    25501 atgccgcatt ccgtcagggc gagcataccg gtattcagct gattctcagc ggtgaagaaa
    25561 gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa
    25621 ccattgctca gttgaaacaa acgcgtgaag aagtcgctat gcaacgcgcc gagctggaag
    25681 agaaacagag cgagcaacaa acgctgttgt atgagcagcg cgcccaacag gcgaagctga
    25741 cccaggcgct gaacgaacgt aaaaagacgc tggcagggct ggagtcttcc atccagcaag
    25801 gtcagcaaca gttgagcgag ctgcgcgcca acgaatcccg tctgcgtaac agcattgccc
    25861 gtgcggaagc cgcggcgaaa gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc
    25921 gcgaccgcca gaaagaagcg acgcgcaaag gcaccaccta caaaccgacc gaaagcgaaa
    25981 aatcgctgat gtcccgaact ggtggcctgg gtgcgccgcg tggtcaggca ttctggccag
    26041 ttcgcgggcc gacgctgcat cgctatggcg agcagctaca gggtgaatta cgctggaaag
    26101 gaatggttat cggtgcctct gaaggtactg aagttaaagc aattgccgac ggtcgggtga
    26161 ttctggctga ctggctgcaa ggctacggtc tggtggtggt ggttgagcat ggtaaaggcg
    26221 acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg
    26281 cgggccagcc gattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt
    26341 tcgaaattcg ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt
    26401 tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg
    26461 taattgctgg caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa
    26521 accaggtgct ggcgatgcct tccgctatct ccgtcgctgt attacccgat tcaccacacg
    26581 ccagagagat ggcgaccaaa gcgcataaca gcggtcacga agtgttgatt catctaccga
    26641 tggcaccact gagtaaacag ccgctggaga aaaatacgct acgcccggag atgagcagcg
    26701 acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca
    26761 acaaccacat gggcagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg
    26821 cgctggagcg ttacaatctt tacttcctcg acagcgtaac catcggtaat acccaagcga
    26881 tgcgcgccgc acaaggcact ggcgtgaagg tgatcaaacg gaaagtgttc ctcgacgatt
    26941 cgcaaaatga agcggacatc cgtgtgcagt tcaatcgcgc aattgacctc gcgcgtcgca
    27001 acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga
    27061 tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac
    27121 cgcaggttga tatgtctaca ccgccgaaaa atactgtgcc cgacacaccg cgtaatccgt
    27181 tccgtggcgt gaagctctgc aaaccgaaga aaccgataga acctgtttat gctaatcgct
    27241 tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc
    27301 agtggcaagg ctggggcaaa cagcccgaag cggcaaaact taacgctagc gcaaattaaa
    27361 ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa
    27421 agaccacagt aacgcctggt aacccacctt cacgctgcgc acattagtaa tcaggcgctt
    27481 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    27541 tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    27601 ataaatcgta attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt
    27661 aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaacc gactcactga
    27721 cgtattatgc agataatatt tatataatga ttgctcggta tatcgcgcac gcagcgcatt
    27781 aaacatgaat tctgttgtcc agacaatatc ctgatgatgt aatccggcaa taaatttaat
    27841 gttattttta acaataacat cgcggcgata aacccccatc cagacaacgt gagtccaacg
    27901 gcgggaagaa agccccatcc gcagccagtc cgggccggtt aatacaccgg ttgagcgaag
    27961 gcgatcgctg gggatggatt gccaggtttc tcccgtttca cgaaaacacc agtcagcgtt
    28021 gcactgcgcc acgtcgaggt cgtcctctaa cgccatggtc atcagcgttt cgtacatggt
    28081 gggatacacc tcatcgtcag catcgacaaa agcgacatat ttgcccgtcg ccgcctcgat
    28141 cccacgatta cgcgccaccg atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg
    28201 atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac catcgttaat
    28261 aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac aagtgcggaa
    28321 atcatcgccc gcattatata acggaataat aacactaagt ttattggtgc tgttcatcat
    28381 aaattaccga cgacctctga agttatcctg acgttttaca ggctttgtat taagaaacta
    28441 ttaagcctgt gagatattat ttttacaggt gagagagtca cttcagggta gagagataat
    28501 gagaacagct atataagaga tcagcgcaat tacgaactct ttattacact gacaggattt
    28561 tacatattta acggattatc ctcaggaaaa agggctggaa taccagccct tttttgtgtt
    28621 aatcccagct caggataact ttcccggact ggcccgaacg catggcgtca aagcccttct
    28681 ggaaatcatc gatagagaaa cgatgagtaa tgatcggcga aagatcgagg ccagactgaa
    28741 tcagcgccgc catcttgtac caggtttcaa acatctcacg accgtaaata cctttaatga
    28801 acaagccttt aaagatcact ttggtccagt cgatagacat atcagacggt ggaataccca
    28861 gcatcgcaat acgaccgccg tggttcatgg tgtcgagcat ggtacgaaac gctggcggcg
    28921 caccggacat ttccagaccg acatcaaagc cttcggtcat gcccagttct gccatcacat
    28981 cattgaggtt ctctttagcg acgttaaccg cacgggtgat acccatttta cgcgccaact
    29041 caaggcggta ttcgttaaca tcagtaatca ccacgttacg tgcaccaacg tgtttcgcca
    29101 ccgccgccgc cataatacca atcgggcctg caccagagac cagcacatcc tcgcccacca
    29161 gatcaaacga cagcgcggta tgcacggcgt taccgaaggg atcaaaaatg gaagccagat
    29221 catcggaaat attgtcgggg attttgaaag cgttgaacgc cggaatcacc agatactcgg
    29281 caaagcagcc cggacggtta acgccgacgc cgatcgtgtt gcggcacaga tgggtacgac
    29341 cgccacgaca gttgcgacag tgaccgcagg tgatatggcc ttcgccagaa acgcgatcgc
    29401 caatcttgaa acctttcact tcctgaccaa tacctaccac ttcaccgaca tattcatggc
    29461 ccacgaccat cggcaccggg atggtttttt gcgaccactc atcccagtta tagatgtgaa
    29521 cgtcagtccc gcagatggct gttttacgga ttttaatcag cagatcgtta tgcccgagtt
    29581 ccggtacagg aacgtcggtc atccagatgc cctcttccgc tttcagtttg gataacgctt
    29641 tcatctcaca tcctcaggcg ataacgccca gttgtttacc aatacgcgtg aatgcttcta
    29701 ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga atacgcgcct
    29761 gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatcccctct ttttgcagct
    29821 cacgagcaaa tttctgcgct actaccgcat cgccaagcat gaccggaata atggcgtgat
    29881 cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactga cgcgcgttcg
    29941 cccacaggcg gtcacgcagt tcgctgcccg cttcgaccat ctccagcact ttgatagacg
    30001 cggcaacaat ggccggtgcc agcgagttgg agaacaggta cggacgagaa cgctggcgca
    30061 gccactcaac cacttctttg cgcgccgccg tgtagccgcc agaagcgcca cccagcgctt
    30121 taccgagcgt gccggtgata atatcgaccc ggcccatcac atcgcagtat tcatgggaac
    30181 cacgaccgtt ttcaccgaca aaacctaccg cgtgtgagtc atctaccatc accagggcat
    30241 catatttatc cgccagatcg caaacgcctt tcaggttggc aatcacgccg tccatcgaga
    30301 acacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct tctttcagac
    30361 gtgcttccag ctcctgcata tcgttgttgg cataacgata gcgtttagct ttgcacagac
    30421 gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc
    30481 ccaacagcgt ttcgaacagg ccaccgttag catcaaagca ggaagagtag agaatcgcat
    30541 cttccatccc caggaaggcc gccagttttt gttcaagctc tttatggctg tcctgagtgc
    30601 cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc gcctttgccg
    30661 ccgcaatcag atccggatga ttcgccagtc cgagatagtt attggcacaa aagttaatga
    30721 cgtggcttcc atcagccaca gtgatatctg cttgctgcgc agacgttata atgcgctctt
    30781 ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt
    30841 ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc caaacttata
    30901 ggtcgcgacg agatgacgcg ttaacacttc tgcaaaattc aggataaata acgtccatca
    30961 atctcacttt tggcgaatgg ctgaagccaa cgccgtgtag tgatatgata gaagtattcg
    31021 tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca tcgttaccgg
    31081 cggcgcgggc tttatcggca gcaacatcgt taaagccctg aatgataaag gcatcaccga
    31141 tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg tggatctgga
    31201 tatcgcggac tatatggata aggaagactt cctgatccag attatggctg gcgaagagtt
    31261 cggcgatgtc gaagcgattt tccacgaagg tgcgtgctct tccaccaccg agtgggacgg
    31321 caagtatatg atggataaca actatcaata ctccaaagag ctgctgcact actgtctgga
    31381 gcgcgaaatc ccgttcctgt atgcctcttc cgcagccacc tacggcggac gcacctccga
    31441 ctttattgaa tcccgcgagt acgaaaaacc gttgaatgtc tacggttact caaaattcct
    31501 gtttgatgaa tatgttcgtc aaatcctgcc agaagcgaac tcgcagattg ttggcttccg
    31561 ctatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt
    31621 ccatctcaac actcagctta acaacggtga atcgccgaag ctgttcgaag gtagcgagaa
    31681 cttcaaacgc gacttcgttt acgtaggcga cgtggcagat gtaaacctgt ggttcctgga
    31741 aaatggcgtt tccggcatct tcaacctcgg tactggtcgt gcggaatcct tccaggcggt
    31801 agcagatgct acgcttgctt atcacaagaa aggccaaatc gaatacattc cgttcccgga
    31861 taaactgaaa ggccgctacc aggcgttcac gcaggcagat ctgacaaatc tgcgcgcggc
    31921 gggttacgac aaaccgttca aaaccgttgc cgaaggtgta acggaataca tggcttggct
    31981 gaatcgcgac gcataagagc tctgcatgaa aatactggtg atcggcccgt cttgggttgg
    32041 cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc cccaggcgat
    32101 aatcgacgtg atggcaccgg catggtgccg tccattatta tcgcggatgc cggaagttaa
    32161 cgaagctatt cctatgcctc tcggtcacgg agcgctggaa atcggcgaac gccgcaaact
    32221 gggtcatagt ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccca actccttcaa
    32281 atccgcatta gtgcctttct tcgcgggtat tcctcatcgc accggctggc gcggcgagat
    32341 gcgctacggt ttacttaacg atgtacgcgt gctcgataaa gaagcctggc cgctaatggt
    32401 ggaacgctat gtcgcgctgg cctatgacaa aggcattatg cgtacagcac aagatctgcc
    32461 gcagccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa
    32521 tcaattttcg ctttcatcag aacgtccgat gattggcttt tgcccgggtg cggagtttgg
    32581 tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc tgattgatga
    32641 aggttatcag gtggttctgt ttggctcggc gaaagatcat gaagcgggca atgagattct
    32701 tgccgctttg aataccgagc agcaggcatg gtgccggaac ctggcggggg aaacacagct
    32761 tgatcaagcg gttatcctga ttgcagcctg taaagccatt gttactaacg attctggtct
    32821 gatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggtc cgagtagccc
    32881 ggacttcaca ccaccgctat cccataaagc gcgcgtgatc cgtctgatta ccggctatca
    32941 caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttgatcg acattactcc
    33001 ccagcgcgta ctggaagaac tcaacgcgct attgttacaa gaggaagcct gacggatgcg
    33061 ggttttgatc gttaaaacat cgtcgatggg cgatgttctc catacgctgc ccgcactcac
    33121 tgatgcccag caggcaatcc cagggattaa gtttgactgg gtggtggaag aagggttcgc
    33181 acagattcct tcctggcacg ccgccgttga gcgagttatt cctgtggcaa tacgtcgctg
    33241 gcgtaaagcc tggttctcgg cccccataaa agctgaacgc aaagcgtttc gtgaagcgct
    33301 acaagcagag aactatgacg cagttatcga cgctcagggg ctggtaaaaa gcgcggcgct
    33361 ggtgacgcgt ctggcgcatg gcgtaaagca tggcatggac tggcaaaccg ttcgagaacc
    33421 gttagccagc ctgttttaca atcgtaagca tcatattgca aaacagcagc acgccgtaga
    33481 acgcacccgc gaactgtttg ccaaaagttt gggctatagc aaaccgcaaa cccagggtga
    33541 ttatgctatc gcacaacatt tcctgacgaa cctgcctaca gatgctggcg aatatgccgt
    33601 atttcttcat gcgacgaccc gtgatgataa acactggccg gaagaacact ggcgagaatt
    33661 gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg cgccgcatga
    33721 ggaagaacgg gcgaaacgac tggcggaagg atttgcttat gttgaagtat tgccgaagat
    33781 gagtctggaa ggcgttgccc gcgtactggc cggggctaaa tttgtagtgt cggtggatac
    33841 ggggttaagc catttaacgg cggcactgga tagaccgaat atcacggttt atggaccaac
    33901 cgatccggga ttaattggcg gatatgggaa gaatcaggtg gtttgtaggg ctccgctaat
    33961 gaatttaaat aatttagaag cagcggctgt ttataaaaaa atcacattaa tttaataaat
    34021 agtaagtggg cattataccc acttacttag tcaacgcttt tattattgtg gttcattttt
    34081 actcaaaaac gatatagaca ggaagaggca cacaatccac gatgccggta ttggtttagc
    34141 atgtgtaatg acatcactaa tgccaaataa cataatagaa gcagggaaaa ccaagagtat
    34201 atattttttc tctcgtatac agtaatagat cattgcaaca tagaacataa taagaacaag
    34261 aagaccaatt tttcctttcg tggaaagaga ttccacaatt tcgttatgca tatgtacatc
    34321 aaggaataat aaagctccac tataaatatt attttctgca cttaatgctt ttatcttttc
    34381 agcacgctgc tcaagagatt gccaattaaa accatcagga cttcctttaa ttcctgcatt
    34441 aaccattgca aaacgtgctc cgaccgagga ttgactgtta ttttttgtag catatgaaat
    34501 aacatcagct tttaatgaat taattcgcct atcaatttcc ttattaaata tcaaaccaca
    34561 agcaagtagc aatataaaaa caccactcaa tcctttaaat aaaaacactt tttgatcacg
    34621 atgctgaaga aataatatga ccataatcat aagtgggaat gtgaaaattg ccgcacgcgt
    34681 tcctgtcatc attactgctg caaagcttaa agcaaagtta gcataaaata gaaaccattt
    34741 atatttcgat tgcattttta tgaatacaat tgaagaatat aaagttgtaa atgtaatcac
    34801 ataagccgca atagtcgctg cccccatctg accaatattc ccattcatgg cggagagggg
    34861 aatgcggtct gcatgcaaat atatcccttg ataatatgct ctacatagca tgattatttg
    34921 aactaagata gctgaaataa aatgtagttt ttcattttgc ctgaatttat ttttacttag
    34981 taataaaaat gtcaacgagc taaatactaa tagttttcca gactcaaggt agcctcg
//
LOCUS       DAOTRK010000037        34721 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.037, whole genome shotgun sequence.
ACCESSION   DAOTRK010000037 DAOTRK010000000
VERSION     DAOTRK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.037"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..795)
                     /gene="map"
                     /locus_tag="QIE41_001755"
     CDS             complement(1..795)
                     /gene="map"
                     /locus_tag="QIE41_001755"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDS3530650.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSPETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            1163..1888
                     /gene="rpsB"
                     /locus_tag="QIE41_001756"
     CDS             1163..1888
                     /gene="rpsB"
                     /locus_tag="QIE41_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDS3530651.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            2146..2997
                     /gene="tsf"
                     /locus_tag="QIE41_001757"
     CDS             2146..2997
                     /gene="tsf"
                     /locus_tag="QIE41_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDS3530652.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            3144..3869
                     /gene="pyrH"
                     /locus_tag="QIE41_001758"
     CDS             3144..3869
                     /gene="pyrH"
                     /locus_tag="QIE41_001758"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDS3530653.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            4161..4718
                     /gene="frr"
                     /locus_tag="QIE41_001759"
     CDS             4161..4718
                     /gene="frr"
                     /locus_tag="QIE41_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDS3530654.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            4810..6006
                     /gene="ispC"
                     /locus_tag="QIE41_001760"
     CDS             4810..6006
                     /gene="ispC"
                     /locus_tag="QIE41_001760"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDS3530655.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            6195..6953
                     /gene="ispU"
                     /locus_tag="QIE41_001761"
                     /gene_synonym="uppS"
     CDS             6195..6953
                     /gene="ispU"
                     /locus_tag="QIE41_001761"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDS3530656.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            6966..7823
                     /gene="cdsA"
                     /locus_tag="QIE41_001762"
     CDS             6966..7823
                     /gene="cdsA"
                     /locus_tag="QIE41_001762"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDS3530657.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWSVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            7835..9187
                     /gene="rseP"
                     /locus_tag="QIE41_001763"
     CDS             7835..9187
                     /gene="rseP"
                     /locus_tag="QIE41_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDS3530658.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWTFEPDKEDPITSLGIRPR
                     GPQIESVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            9217..11649
                     /gene="bamA"
                     /locus_tag="QIE41_001764"
     CDS             9217..11649
                     /gene="bamA"
                     /locus_tag="QIE41_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDS3530659.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            11771..12256
                     /gene="skp"
                     /locus_tag="QIE41_001765"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             11771..12256
                     /gene="skp"
                     /locus_tag="QIE41_001765"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDS3530660.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            12260..13285
                     /gene="lpxD"
                     /locus_tag="QIE41_001766"
     CDS             12260..13285
                     /gene="lpxD"
                     /locus_tag="QIE41_001766"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDS3530661.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSRIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            13390..13845
                     /gene="fabZ"
                     /locus_tag="QIE41_001767"
     CDS             13390..13845
                     /gene="fabZ"
                     /locus_tag="QIE41_001767"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDS3530662.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            13849..14637
                     /gene="lpxA"
                     /locus_tag="QIE41_001768"
     CDS             13849..14637
                     /gene="lpxA"
                     /locus_tag="QIE41_001768"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDS3530663.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            14637..15785
                     /gene="lpxB"
                     /locus_tag="QIE41_001769"
     CDS             14637..15785
                     /gene="lpxB"
                     /locus_tag="QIE41_001769"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDS3530664.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            15782..16378
                     /gene="rnhB"
                     /locus_tag="QIE41_001770"
     CDS             15782..16378
                     /gene="rnhB"
                     /locus_tag="QIE41_001770"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDS3530665.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPSRPIAGLN
                     DSKKLSEKRRLALCEEIKDKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            16415..19897
                     /gene="dnaE"
                     /locus_tag="QIE41_001771"
     CDS             16415..19897
                     /gene="dnaE"
                     /locus_tag="QIE41_001771"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDS3530666.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVMRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            19910..20869
                     /gene="accA"
                     /locus_tag="QIE41_001772"
     CDS             19910..20869
                     /gene="accA"
                     /locus_tag="QIE41_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDS3530667.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            20967..23108
                     /gene="ldcC"
                     /locus_tag="QIE41_001773"
     CDS             20967..23108
                     /gene="ldcC"
                     /locus_tag="QIE41_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDS3530668.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            23165..23554
                     /locus_tag="QIE41_001774"
     CDS             23165..23554
                     /locus_tag="QIE41_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDS3530669.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            23619..24917
                     /gene="tilS"
                     /locus_tag="QIE41_001775"
     CDS             23619..24917
                     /gene="tilS"
                     /locus_tag="QIE41_001775"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDS3530670.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRAENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQKPQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(24966..25226)
                     /gene="rof"
                     /locus_tag="QIE41_001776"
     CDS             complement(24966..25226)
                     /gene="rof"
                     /locus_tag="QIE41_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDS3530671.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(25213..25413)
                     /locus_tag="QIE41_001777"
     CDS             complement(25213..25413)
                     /locus_tag="QIE41_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDS3530672.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            25579..26124
                     /locus_tag="QIE41_001778"
     CDS             25579..26124
                     /locus_tag="QIE41_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDS3530673.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            26121..26543
                     /gene="arfB"
                     /locus_tag="QIE41_001779"
     CDS             26121..26543
                     /gene="arfB"
                     /locus_tag="QIE41_001779"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDS3530674.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            26557..27267
                     /gene="nlpE"
                     /locus_tag="QIE41_001780"
                     /gene_synonym="cutF"
     CDS             26557..27267
                     /gene="nlpE"
                     /locus_tag="QIE41_001780"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDS3530675.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLTARGHSEKPMLLSLEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(27298..28122)
                     /locus_tag="QIE41_001781"
     CDS             complement(27298..28122)
                     /locus_tag="QIE41_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDS3530676.1"
                     /translation="MDKPKVHCRLLLPCFLFLSACTVDISQPDSSATAVDAGAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSHGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(28175..29893)
                     /gene="proS"
                     /locus_tag="QIE41_001782"
     CDS             complement(28175..29893)
                     /gene="proS"
                     /locus_tag="QIE41_001782"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDS3530677.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(30004..30711)
                     /gene="tsaA"
                     /locus_tag="QIE41_001783"
     CDS             complement(30004..30711)
                     /gene="tsaA"
                     /locus_tag="QIE41_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDS3530678.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(30708..31112)
                     /gene="rcsF"
                     /locus_tag="QIE41_001784"
     CDS             complement(30708..31112)
                     /gene="rcsF"
                     /locus_tag="QIE41_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDS3530679.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(31230..32045)
                     /gene="metQ"
                     /locus_tag="QIE41_001785"
     CDS             complement(31230..32045)
                     /gene="metQ"
                     /locus_tag="QIE41_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDS3530680.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(32085..32738)
                     /gene="metI"
                     /locus_tag="QIE41_001786"
     CDS             complement(32085..32738)
                     /gene="metI"
                     /locus_tag="QIE41_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDS3530681.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(32731..33762)
                     /gene="metN"
                     /locus_tag="QIE41_001787"
     CDS             complement(32731..33762)
                     /gene="metN"
                     /locus_tag="QIE41_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDS3530682.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            33949..34521
                     /gene="gmhB"
                     /locus_tag="QIE41_001788"
     CDS             33949..34521
                     /gene="gmhB"
                     /locus_tag="QIE41_001788"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDS3530683.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         8390 a         8490 c         9538 g         8303 t
ORIGIN      
        1 ttattcgtcg tgcgagatta tcgccgggat ggtgtcatcc ttgcgtagcg tcagaatttc
       61 gcagccgtta tcagtcacca caatagtatg ctcatattgt gcagacaagc tgcgatcttt
      121 ggtttttacc gtccagccat ctttcatggt gcggatctct tttttacccg cgttgaccat
      181 tggctcgatg gtgaaggtca tccccggttt cagcacgacg ttggtttcag gggagtcata
      241 gtgcagtacc tgcggttctt catggaagcc acgaccaata ccgtgtccac aatattcacg
      301 aacgacggag aaaccttctg cttcaacaaa tttctgaatc gccgcaccga tttcgcgcag
      361 attaatgcct ggtttgacca tgcgcagcgc caggtacagg ctttcttgcg tgatgcggca
      421 cagacgttcg cccatgatgg tcggcttacc gacgataaac attttcgagg tatcgccgtg
      481 gaaaccatct ttgattacgg tgacatcaat gttaacgata tcgccatctt tcagcagctt
      541 ggcgtcgtcc gggataccgt ggcacaccac ttcattaata gagatgcaga cggatttcgg
      601 atagccgtga tagccgaggc aggcagaaac cgcgtgttgt tcattaacaa tgtaatcatt
      661 acagatgcga tccagctcgc cggtgctgac gcccggttta acatacggtt cgatcatctc
      721 cagcacttcg gcagccagtc ggccagcgac gcgcattttt tcgatatctt ctggggtctt
      781 gattgagata gccattaatt ctgtccatca gcgtcggtga taccgacaat atatatgtaa
      841 gtgccgtcaa tggtatcaca cccgggcaaa ttgagaatca ttctgaattt cgccaaacgt
      901 gccactgaag gttttctata atagaaaatt caacgtctga tgctgtacac agcgccaaca
      961 attattggtg tccgcgacgt atttgtggta taaagcgcgc cggacttccg atccatttcg
     1021 tatacacaga ctggacggaa gcgacaatct cactttgtgt aacaacacac acgtatcggc
     1081 acatattccg gggtgccctt tggggtcggt aatatgggat acgtggaggc ataaccccaa
     1141 cttttatata gaggttttaa tcatggcaac tgtttccatg cgcgacatgc tcaaggctgg
     1201 tgttcacttc ggtcaccaga cccgttactg gaacccgaaa atgaagccgt tcatcttcgg
     1261 tgcgcgtaac aaagttcaca tcatcaacct tgagaaaact gtaccgatgt tcaacgaagc
     1321 tctggctgaa ctgaacaaga ttgcttctcg caaaggtaaa atccttttcg ttggtactaa
     1381 acgcgctgca agcgaagcgg tgaaagacgc tgctctgagc tgcgaccagt tcttcgtgaa
     1441 ccatcgctgg ctgggcggta tgctgactaa ctggaaaacc gttcgtcagt ccatcaaacg
     1501 tctgaaagac ctggaaactc agtctcagga cggtactttc gacaagctga ccaagaaaga
     1561 agcgctgatg cgcactcgtg agctggagaa actggaaaac agcctgggcg gtatcaaaga
     1621 catgggcggt ctgccggacg ctctgtttgt aatcgatgct gaccatgaac acattgctat
     1681 caaagaagca aacaacctgg gtattccggt atttgctatc gttgatacca actctgatcc
     1741 ggacggtgtt gacttcgtta tcccgggtaa cgacgacgca atccgtgctg tgaccctgta
     1801 cctgggcgct gttgctgcaa ccgtacgtga aggccgttct caggatctgg cttcccaggc
     1861 ggaagaaagc ttcgtagaag ctgagtaata aggcttgata actcccccaa aatagttcga
     1921 gttgcagaaa ggcggcaagc tcgagaattc ccgggagctt acatcagtaa gtgaccggga
     1981 tgagcgagcg aagacaacgc atctgcggcg cgaaatatga agggggagag cccttataga
     2041 ccaggtagta cacgtttggt tagggggcct gcatatggcc ccctttttca cttttatatc
     2101 tgtgcggttt aatgccgggc agatcacatc tccgaggatt ttagaatggc tgaaattacc
     2161 gcatccctgg taaaagagct gcgtgagcgt actggcgcag gcatgatgga ttgcaaaaaa
     2221 gcactgactg aagctaacgg cgacatcgag ctggcaatcg aaaacatgcg taagtccggt
     2281 gctattaaag cagcgaaaaa agcaggcaac gttgctgctg acggcgtgat caaaaccaaa
     2341 atcgacggca actacggcat cattctggaa gttaactgcc agactgactt cgttgcaaaa
     2401 gacgctggtt tccaggcgtt cgcagacaaa gttctggacg cagctgttgc tggcaaaatc
     2461 actgacgttg aagttctgaa agcacagttc gaagaagaac gtgttgcgct ggtagcgaaa
     2521 attggtgaaa acatcaacat tcgccgcgtt gctgcgctgg aaggcgacgt tctgggttct
     2581 tatcagcacg gtgcgcgtat cggcgttctg gttgctgcta aaggcgctga cgaagagctg
     2641 gttaaacaca tcgctatgca cgttgctgca agcaagccag aattcatcaa accggaagat
     2701 gtatccgctg aagtggtaga aaaagaatac caggtacagc tggatatcgc gatgcagtct
     2761 ggtaagccga aagaaatcgc agagaaaatg gttgaaggcc gcatgaagaa attcaccggc
     2821 gaagtttctc tgaccggtca gccgttcgtt atggaaccaa gcaaaactgt tggtcagctg
     2881 ctgaaagagc ataacgctga agtgactggc ttcatccgct tcgaagtggg tgaaggcatc
     2941 gagaaagttg agactgactt tgcagcagaa gttgctgcga tgtccaagca gtcttaatta
     3001 tcgaaaagga gccgcctgag ggcggcttct ttttgtgccc atcttgtaaa ttcagctaac
     3061 ccttgtgggg ctgcgctgaa aagcgacgta caatgtcgct agtattaatt catttcaatc
     3121 gttgacagtc tcaggaaaga aacatggcta ccaatgcaaa acccgtctat aaacgcattc
     3181 tgcttaagtt gagtggcgaa gctctgcagg gcactgaagg cttcggtatt gatgcaagca
     3241 tactggatcg catggctcag gaaatcaaag aactggttga actgggtatt caggttggtg
     3301 tggtgattgg tgggggtaac ctgttccgtg gcgctggtct ggcgaaagcg ggtatgaacc
     3361 gcgttgtggg cgaccacatg gggatgctgg cgaccgtaat gaacggcctg gcaatgcgtg
     3421 atgcactgca ccgcgcctat gtgaacgctc gtctgatgtc cgctattcca ttgaatggcg
     3481 tgtgcgacag ctacagctgg gcagaagcta tcagcctgtt gcgcaacaac cgtgtggtga
     3541 tcctctccgc cggtacaggt aacccgttct ttaccaccga ctcagcagct tgcctgcgtg
     3601 gtatcgaaat tgaagccgat gtggtgctga aagcaaccaa agttgacggc gtgtttaccg
     3661 ctgatccggc gaaagatcca accgcaacca tgtacgagca actgacttac agcgaagtgc
     3721 tcgaaaaaga gctgaaagtc atggacctgg cggccttcac gctggctcgt gaccataaat
     3781 taccgattcg tgttttcaac atgaacaaac cgggtgcgct gcgccgtgtg gtaatgggtg
     3841 aaaaagaagg gactttaatc acggaataat tcccgtgatg gataaataag ggtaagattc
     3901 cgcgtaagta tcgcgggggc gtaagtctgg ttataaggcg ttattgttgc aggcagtttg
     3961 gtcacggcca gcgcgcagca accggagcgt acacaagtgc gtgaggatgg cgagcactgc
     4021 ccggggccaa aatggcaaat aaaatagcct aataatccag acgattaccc gtaatatgtt
     4081 taatcagggc tatacttagc acacttccac tgtgtgtgac tgtctggtct gactgagaca
     4141 agttttcaag gattcgtaac gtgattagcg atatcagaaa agatgctgaa gtacgcatgg
     4201 acaaatgcgt agaagcgttc aaaacccaaa tcagcaaaat acgcacgggt cgtgcttctc
     4261 ccagcctgct ggatggcatt gtcgtggaat attacggcac gccgacgccg ctgcgtcagc
     4321 tggcaagcgt aacggtagaa gattcccgta ctctgaaaat caacgtgttt gatcgttcaa
     4381 tgtctccggc cgttgaaaaa gcgattatgg cgtccgatct tggcctgaac ccgaactctg
     4441 cgggtagcga catccgtgtt ccgctgccgc cgctgacgga agaacgtcgt aaagatctga
     4501 ccaaaatcgt tcgtggtgaa gcagaacaag cgcgtgttgc agtacgtaac gtgcgtcgtg
     4561 acgcgaacga caaagtgaaa gcactgttga aagataaaga gatcagcgaa gacgacgatc
     4621 gccgttctca ggacgatgta cagaaactga ctgatgctgc aatcaagaaa attgaagcgg
     4681 cgctggcaga caaagaagca gaactgatgc agttctgatt tcttgaacga caaaaacgcc
     4741 gctcagtaga tccttgcgga tcggctggcg gcgttttgct ttttattctg tctcaactct
     4801 ggatgtttca tgaagcaact caccattctg ggctcgaccg gctcgattgg ttgcagcacg
     4861 ctggacgtgg tgcgccataa tcccgaacac ttccgcgtag ttgcgctggt ggcaggcaaa
     4921 aatgtcactc gcatggtaga acagtgcctg gaattctctc cccgctatgc cgtaatggac
     4981 gatgaagcga gtgcgaaact tcttaaaacg atgctacagc aacagggtag ccgcaccgaa
     5041 gtcttaagtg ggcaacaagc cgcttgcgat atggcagcgc ttgaggatgt tgatcaggtg
     5101 atggcagcca ttgttggcgc tgctgggctg ttacctacgc ttgctgcgat ccgcgcgggt
     5161 aaaaccattt tgctggccaa taaagaatca ctggttacct gcggacgtct gtttatggac
     5221 gccgtaaagc agagcaaagc gcaattgtta ccggtcgata gcgaacataa cgccattttt
     5281 cagagtttac cgcaacctat ccagcataat ctgggatacg ctgaccttga gcaaaatggc
     5341 gtggtgtcca ttttacttac cgggtctggt ggccctttcc gtgagacgcc attgcgcgat
     5401 ttggcaacaa tgacgcccga tcaagcctgc cgtcatccga actggtcgat gggacgtaaa
     5461 atttccgtcg attcggctac catgatgaac aaaggtctgg aatacattga agcgcgttgg
     5521 ctgtttaacg ccagcgccag ccagatggaa gtgctgattc acccgcagtc agtgattcac
     5581 tcaatggtgc gctaccagga cggcagtgtt ctggcgcagc tgggtgaacc ggatatgcgt
     5641 acgccaattg ctcacaccat ggcatggccg aatcgcgtga actctggcgt gaagccactc
     5701 gatttttgca aactaagtgc gttgacattt gccgcaccgg attatgatcg ttatccatgc
     5761 ctgaaactgg cgatggaggc gttcgaacaa ggccaggcag cgacgacagc attgaatgcc
     5821 gcaaacgaaa tcaccgttgc tgcttttctt gcgcaacaaa tccgctttac ggatatcgcc
     5881 gcgttgaatt tatccgtact ggaaaaaatg gatatgcgcg aaccacaatg tgtggacgat
     5941 gtgttatctg ttgatgcgaa cgcgcgtgaa gtcgccagaa aagaggtgat gcgtctcgca
     6001 agctgaggat aatccggcta cagagagtcg cgctatttgt tagcgtaggg cttcagtgat
     6061 atagtctgcg ccatctgatc gtaagtagtt ggctttataa ggtcaggtat gccgtggttt
     6121 tacacggctt ttttttgtat aggcttcagt attcctgagt accgtaaacc ctgtcaggga
     6181 ataagaaacg cgtgatgttg tctgctactc aaccacttag cgaaaaattg ccagcgcatg
     6241 gctgtcgtca tgttgcgatc attatggacg gcaatggtcg ctgggcaaaa aagcaaggga
     6301 agattcgtgc ctttgggcat aaagccgggg caaaatccgt ccgccgggct gtctcttttg
     6361 cggccaacaa cggtattgag gcgttaactc tgtatgcctt tagtagtgaa aactggaacc
     6421 gaccagcgca ggaagtcagt gcgttaatgg aactgtttgt gtgggcgctt gatagcgaag
     6481 taaagagtct gcaccgacat aacgtgcgtc tgcgtattat tggcgatacc agtcgcttta
     6541 actcgcgttt gcaagaacgt attcgtaaat ctgaagcgct aacagccggg aataccggtc
     6601 tgacgctgaa tattgcggcg aactacggtg gacgttggga tatagtccag ggagtcaggc
     6661 aactggctga aaaggtgcag caaggaaacc tgcaaccaga tcagatagat gaagagatgc
     6721 taaaccagca tgtctgtatg catgaactgg cccctgtaga tttagtaatt aggactgggg
     6781 gggagcatcg cattagtaac tttttgcttt ggcaaattgc ctatgccgaa ctttacttta
     6841 cagatgttct ctggcccgat ttcgatgaac aagactttga aggggcgtta aatgcctttg
     6901 ctaatcgaga gcgtcgtttc ggcggcaccg agcccggtga tgaaacagcc tgatgggggt
     6961 cgcttttgct gaagtatcgc ctgatatctg cttttgtgtt aatacccgtc gtcatcgcgg
     7021 cgttgtttct gttgccgccg gtggggttcg ccattgtaac gctggtggtc tgcatgctgg
     7081 cagcgtggga atggggacag cttagcggtt ttaccactcg ctcgcagcga gtatggttgg
     7141 cggtgttatg tgggttattg ttggcgctga tgctttttct gttgccggaa tatcaccgaa
     7201 atattcatca accgctggtt gaaatctcac tttgggcttc gctgggttgg tggagtgtcg
     7261 cgctattgct ggtgctgttt tacccaggtt ccgcagcaat ctggcgtaac tctaaaacat
     7321 tgcgcattat ttttggcgtg ctaaccattg ttcccttctt ctggggcatg ctggcgttac
     7381 gggcctggca ctacgacgag aatcattaca gtggcgcaat atggctgctc tatgtcatga
     7441 tcctggtatg gggcgctgac tctggcgcat atatgtttgg caaattgttt ggtaaacata
     7501 aactggcacc gaaggtttct ccgggtaaaa cctggcaagg ctttataggt ggactcgcga
     7561 ctgcagcggt aatctcatgg ggttatggca tgtgggcgaa tctcgacgtc gctcccgtca
     7621 ccttactcat ttgctctatt gtcgcagcgt tagcctcagt gctcggcgat ctgaccgaga
     7681 gtatgtttaa gcgtgaagca ggaattaagg acagcggtca tttaattcca ggacacggtg
     7741 gtattttaga tcgtattgat agcctgacgg ctgcggtacc ggtctttgct tgcttgttgt
     7801 tactggtatt caggacgctt taacggaagg taatatgctg agttttctct gggatttggc
     7861 ttcgttcatc gttgcactgg gtgtacttat caccgtgcat gaatttggtc atttctgggt
     7921 tgcccggcgt tgtggtgttc gcgttgagcg tttctcaata gggtttggta aggcgctctg
     7981 gcggcgaact gataagctcg gcaccgaata tgttatcgcc ctgatcccgc tgggcggtta
     8041 tgtcaaaatg ctggatgagc gcgcagaacc ggtcgttccg gaactccgcc accatgcctt
     8101 caataataaa tctgtcggcc aacgagcggc gattattgcc gcaggtccgg ttgcaaactt
     8161 catttttgct atctttgcct actggctggt ttttattatt ggtgtgcctg gcgtacgtcc
     8221 ggtggttggc gaaatagcag ccaattcgat agctgcagaa gcacaaattg caccaggtac
     8281 ggaactaaaa gccgtagatg gtatcgaaac gcctgattgg gatgccgtgc gtttgcagtt
     8341 ggtcgataaa attggcgatg aaagcaccac cattacagta gcgccatttg gcagcgacca
     8401 acggcgggat gtaaagctcg atttacgtca ctggacgttt gagcctgata aagaagatcc
     8461 gataacttcg ctggggattc gtcctcgtgg gccgcaaatt gaatctgtac tggaaaatgt
     8521 gcagccaaac tcggcggcaa gcaaggcagg tttgcaagca ggcgacagga tcgttaaagt
     8581 cgatggtcag cccttaacgc agtgggtgac ctttgtgatg cttgtccggg ataacccggg
     8641 taaatcctta gcgttagaaa tcgaaaggca ggggagtccc ttgtctttga cattaatccc
     8701 ggagagtaaa ccgggtaatg gtaaagcgat tggttttgtc ggtattgagc cgaaagtcat
     8761 tcctttgcca gatgagtata aagttgtacg ccagtatggg ccgttcaacg ccatcgtcga
     8821 agccacggac aaaacgtggc agctgatgaa gctgacggtc agtatgctgg gaaaattgat
     8881 caccggtgat gtgaaactga acaacctcag tgggccgatc tctatcgcca agggggctgg
     8941 gatgacagcg gaactcgggg ttgtttatta cctgccgttt cttgcgctta ttagcgtgaa
     9001 cttagggata attaacctgt ttccgttgcc cgtacttgac ggggggcatc tgctgttcct
     9061 tgcgatcgaa aagatcaagg gcggaccggt atccgagcgg gttcaagact tttgttatcg
     9121 cattggctcg attctgctgg tgctgttaat ggggcttgca cttttcaatg atttctctcg
     9181 gttatgagag ttagttagga agaacgcata ataacgatgg cgatgaaaaa gttgctcata
     9241 gcgtcgctgc tgtttagcag cgccaccgta tacggtgctg aagggttcgt agtgaaagat
     9301 attcatttcg aaggccttca gcgtgtcgcc gttggtgcgg ccctcctcag tatgccggtg
     9361 cgcacaggcg acacggttaa tgatgaagat atcagtaata ccattcgcgc tctgtttgct
     9421 accggcaact ttgaggatgt tcgcgtcctt cgtgatggtg atacccttct ggttcaggta
     9481 aaagaacgtc cgaccattgc cagcattact ttctccggta acaaatcggt gaaagatgac
     9541 atgctgaaac aaaacctcga ggcttctggt gtgcgtgtgg gcgaatccct cgatcgcacc
     9601 accattgccg atatcgagaa aggtctggaa gacttctact acagcgtcgg taaatatagc
     9661 gccagcgtta aagctgtcgt gaccccgctg ccgcgcaacc gtgttgacct aaaactggtg
     9721 ttccaggaag gtgtgtcagc tgaaatccag caaattaaca ttgttggtaa ccatgctttc
     9781 accaccgacg aactgatctc tcatttccaa ctgcgtgacg aagtgccgtg gtggaacgtg
     9841 gtaggcgatc gtaaatacca gaaacagaaa ctggcgggcg accttgaaac cctgcgcagc
     9901 tactatctgg accgcggtta tgcccgtttc aacatcgact ctacccaggt cagtctgacg
     9961 ccagataaaa aaggtattta cgtcacggtg aacatcaccg aaggcgatca gtacaagctt
    10021 tctggcgttg aagtgagcgg caaccttgcc gggcactccg ctgaaattga gcagctgact
    10081 aagatcgagc cgggcgagct gtataacggc accaaagtga ccaaaatgga agatgacatt
    10141 aaaaagcttc tcggtcgcta tggttatgcc tatccgcgcg tacagtcgat gcctgaaatt
    10201 aacgatgccg acaaaaccgt taaattacgc gtgaacgttg atgcgggtaa ccgtttctac
    10261 gtgcgtaaga tccgttttga aggtaacgat acctcgaaag atgccgtcct gcgtcgcgaa
    10321 atgcgtcaga tggaaggtgc atggctgggg agcgatctgg tcgatcaggg taaggagcgt
    10381 ctgaatcgtc tgggcttctt tgaaactgtc gataccgata cccaacgtgt tccgggtagc
    10441 ccggaccagg ttgatgtagt ctacaaggtg aaagaacgta acaccggtag cttcaacttc
    10501 gggattggtt acggtactga aagtggcgtg agcttccagg ctggtgtgca acaggataac
    10561 tggttaggta cgggttatgc tgttggtatc aacgggacca aaaacgatta ccagacctat
    10621 gctgaactgt cggtaaccaa cccgtacttc accgtagatg gcgtaagcct cggtggtcgt
    10681 ctcttctata atgacttcca ggcagatgac gccgacctgt ccgactatac caacaagagt
    10741 tatggtacag acgtgacgtt gggcttcccg atcaacgaat ataactcgct gcgtgcaggt
    10801 ctgggttatg tacataactc cctgtccaac atgcagcctc aggttgcgat gtggcgttat
    10861 ctgtactcta tgggtgaaca tccgagcacc tctgatcagg ataacagctt caaaacggac
    10921 gacttcacgt tcaactatgg ttggacctat aacaagcttg accgtggtta cttcccgaca
    10981 gatggttcac gtgtcaacct gactggtaaa gtgaccattc cgggatcgga taacgaatac
    11041 tacaaagtga cgttagacac ggcgacttat gtgccgatcg atgacgatca caaatgggtt
    11101 gttctgggtc gtacccgctg gggttatggt gatggtttag gcggcaaaga gatgccgttc
    11161 tacgagaact tctatgccgg tggttccagc accgtccgtg gcttccagtc caataccatt
    11221 ggtccgaaag cagtttactt cccgcatcag gctagtaatt atgatccgga ctatgattac
    11281 gaatgtgcga ctcaggacgg cgcgaaagac ctgtgtaaat cggatgatgc tgtaggcggt
    11341 aacgctatgg cggttgccag cctcgagttc atcaccccga cgccgtttat tagcgataag
    11401 tatgctaact cggttcgtac ttccttcttc tgggatatgg gtaccgtttg ggatacaaac
    11461 tgggattcca gccaatattc tggatatccg gactatagtg atccaagcaa tatccgtatg
    11521 tctgcgggta tcgcattaca atggatgtcc ccattggggc cgttggtgtt ctcctacgcc
    11581 cagccgttca aaaagtacga tggagacaag gcagaacagt tccagtttaa catcggtaaa
    11641 acctggtaag tgttctccac aaaggaatgt agtggtagtg tagcgatgac tttaggcgat
    11701 caatataaga tcgccgggcc acgcaaagaa ctgcaccctc cggtgcaaat gggatggtaa
    11761 ggagtttatt gtgaaaaagt ggttattagc tgcaggtctt ggtttagcac tggcaacttc
    11821 tgctcaggcg gctgacaaaa ttgcaatcgt caatatgggc agcctgttcc agcaggtagc
    11881 gcagaaaacc ggtgtttcta acacgctgga aaatgagttc aaaggccgtg ccagcgaact
    11941 gcagcgtatg gaaaccgatc tgcaggctaa aatgaaaaag ctgcagtcca tgaaagcggg
    12001 cagcgatcgc accaaactgg aaaaagacgt gatggctcag cgccagactt ttgctcagaa
    12061 agcgcaggct tttgagcagg atcgcgcacg tcgttccaac gaagaacgcg gcaaactggt
    12121 tactcgtatc cagactgctg tgaaatccgt tgccaacagc caggatatcg atctggttgt
    12181 tgatgcaaac gccgttgctt acaacagcag cgatgtaaaa gacatcactg ccgacgtact
    12241 gaaacaggtt aaataagtaa tgccttcaat tcgactggct gatttagcgc agcagttgga
    12301 tgcagaacta cacggtgatg gcgatatcgt catcaccggc gttgcgtcca tgcaatctgc
    12361 acaaacaggt cacattacgt tcatggttaa cccaaaatac cgtgagcatt taggcttgtg
    12421 ccaggcgtcc gcggttgtca tgacccagga cgatcttcct ttcgcgaaaa gtgccgcgct
    12481 ggtagtgaag aatccctacc tgacttacgc gcgcatggcg caaattttag ataccacgcc
    12541 gcagcccgcg cagaacattg cacccagtgc ggtgatcgac gcgacggcga agctgggtaa
    12601 caacgtatcg attggcgcta acgcggtgat tgagtccggc gttgaactgg gcgataacgt
    12661 gattatcggt gccggttgct tcgtaggtaa aaacagcaga atcggtgcag gttcgcgtct
    12721 ctgggcgaac gtaaccattt accatgagat ccagatcggt cagaattgcc tgatccagtc
    12781 cggaacagtg gtaggtgcag acggctttgg ttatgccaac gatcgtggta actgggtgaa
    12841 gatcccacag attggtcgcg taattattgg cgatcgcgtg gagatcggcg catgtactac
    12901 catcgatcgc ggcgcgctgg atgacactgt tattggcaat ggcgtgatca ttgataacca
    12961 gtgccagatt gcacataacg tcgtgattgg cgacaatacg gcggttgccg gtggcgtcat
    13021 tatggcgggc agcctgaaaa ttggtcgtta ctgcatgatc ggcggagcca gcgtaatcaa
    13081 cgggcatatg gaaatatgcg acaaagtgac ggttacgggc atgggtatgg tgatgcgtcc
    13141 catcactgaa ccaggcgtct attcctcagg cattccgctg caacccaaca aagtctggcg
    13201 caaaaccgct gcactggtga tgaacattga tgacatgagc aagcgtctga aatcgcttga
    13261 gcgcaaggtt aatcaacaag actaacgttc catcttttgt tcgccaaact ttacggcctg
    13321 tctcattctt acgattgcgg caggccgtgt tattattgtc gtttcttata ttttgacagg
    13381 aagagtatct tgactactaa cactcatact ctgcagattg aagagatttt agaacttctg
    13441 ccgcaccgtt tcccgttctt actggtggat cgcgtgctgg attttgaaga aggtcgtttt
    13501 ctgcgcgcag taaaaaatgt ctctgtcaat gagccattct tccagggcca tttccctgga
    13561 aaaccgattt tcccgggtgt gctgattctg gaagcaatgg cacaggcaac aggtattctg
    13621 gcgtttaaaa gcgtaggaaa actggaaccg ggtgagctgt actacttcgc tggtattgac
    13681 gaagcgcgct tcaagcgccc ggtcgtgcct ggcgatcaaa tgatcatgga agtcactttc
    13741 gaaaaaacgc gccgcggcct gacccgtttt aaaggggttg ctctggtcga cggtaaagta
    13801 gtttgcgaag caacgatgat gtgtgctcgt agccgggagg cctgatacgt gattgataaa
    13861 tccgcctttg tgcatccaac cgccattgtg gaagagggcg cgtcgattgg cgctaacgct
    13921 cacattggtc ctttttgtat cgttggaccc catgtcgaaa ttggtgaggg taccgtactg
    13981 aaatctcacg ttgtcgtgaa tggtcatact aaaattggcc gcgataatga gatttatcag
    14041 ttcgcctcca tcggcgaagt taaccaggat ctgaaatatg ctggcgaacc gacccgcgtg
    14101 gaaatcggcg atcgtaaccg cattcgcgaa agcgtcacca ttcatcgtgg cacagtccag
    14161 ggcggtggat tgacgaaggt gggcagcgac aacttactga tgatcaacgc gcacattgcg
    14221 cacgattgta cggtaggtaa ccgctgtatt ctcgccaaca acgcaacgct ggcgggtcac
    14281 gtatcggttg acgacttcgc gatcatcggc ggcatgaccg cagtccatca gttctgcatc
    14341 attggtgcgc atgtgatggt tggcggctgc tccggtgtgg cgcaggacgt ccctccttat
    14401 gtcattgcgc agggtaacca cgcaacgccg ttcggtgtca atatcgaagg gctgaagcgc
    14461 cgcggattca gccgtgaggc gattaccgct atccgcaatg cgtataagct gatttatcgt
    14521 agcggtaaaa cgctcgatga agtgaaaccg gaaattgctg aactggcgga aacatatccg
    14581 gaagtgaaag cctttaccga tttctttgca cgctcaacgc gcggtctgat tcgttaatga
    14641 ctgaacagcg tccattaacg attgccctgg tcgccggaga aacctccggc gatatcctgg
    14701 gggccggttt aatccgcgct ctgaaagaac gtgtgcccaa cgcccgcttt gttggtgttg
    14761 ccgggccacg aatgcaggct gaaggctgcg aagcctggta cgaaatggaa gaactggcgg
    14821 tgatgggcat tgttgaagtg ctcggtcgtc tgcgtcgctt actgcatatt cgtgccgatc
    14881 tgacaaagcg ttttggcgaa ctgaagccag atgtttttgt tggtattgat gcgcccgact
    14941 tcaatattac tcttgaaggt aacctcaaaa agcagggtat caaaaccatt cattacgtca
    15001 gtccatccgt ctgggcgtgg cgacagaaac gcgttttcaa aataggcaga gccaccgatc
    15061 tggtgctcgc atttctgcct ttcgaaaaag cgttttatga caaatacaat gtaccgtgcc
    15121 gctttatcgg tcataccatg gctgatgcca tgccattaga tccagataaa aatggtgccc
    15181 gtgatgtgct ggggatccct tacgatgccc actgtctggc tttgttgccg ggcagccgtg
    15241 gtgcagaagt cgaaatgctt agtgctgatt tcctgaaaac ggctcagctt ttgcgccaga
    15301 catatccgga tctcgaaatc gtggtgccgc tggtgaatgc caaacgccgc gagcagtttg
    15361 aacgcatcaa agctgaagtt gcgccagacc tgtcggttca tatgttggat gggatgggcc
    15421 gtgaggcgat ggtcgctagt gatgcggcac tactggcgtc ggggacggca gccctggagt
    15481 gcatgctggc gaaatgcccg atggtggtgg ggtatcgcat gaagcctttc actttctggt
    15541 tggcgaagcg tctggtgaaa actgattatg tctcgctgcc aaatttgctg gcgggcagag
    15601 agttagtcaa agagttattg caggaagagt gtgagccgca aaaactggct gcggcgctgt
    15661 taccgctgtt ggcgaacggg aaaaccagcc acgcgatgca cgataccttc cgtgaactgc
    15721 atcagcagat ccgctgcaat gccgatgagc aggcggcaca agccgttctg gagttagcac
    15781 aatgatcgaa tttgtttatc cgcacacgca gctggttgcg ggtgtggatg aagtcggacg
    15841 cgggccgtta gttggcgcgg tcgtcaccgc tgcggtgatc cttgacccat cgcgcccgat
    15901 tgccgggctg aatgattcca aaaagctgag cgaaaaacgc cgtctggcgc tctgtgaaga
    15961 gatcaaagat aaagcgttga gctggagtct gggccgcgcg gaaccccacg aaatcgacga
    16021 actgaatatt ttacacgcga ccatgctggc gatgcagcgt gccgtcgctg ggctgcatat
    16081 tgcgccggaa tatgtgttga ttgatggtaa ccgctgcccg aaattaccga tgccttcgat
    16141 ggcagtggtg aaaggcgata gccgcgtacc agaaatcagt gctgcgtcta tcctggcgaa
    16201 agtgacgcgc gacgccgaaa tggcggcgct ggatattgtt ttcccgcaat atggttttgc
    16261 ccaacacaaa gggtatccaa ctgcttttca tctggaaaaa ctggctgaat atggcgcgac
    16321 tgaacatcat cgtcgcagct ttgggcctgt caaacgcgca ctgggacttg cgtcctgatt
    16381 cttgtgtcga gattaagtaa accggaatct gaagatgtct gaaccacgtt tcgtacacct
    16441 gcgggtgcac agcgactact cgatgatcga tggtctggcc aaaaccgcac cgctggtaaa
    16501 aaaggcggcg gcgttgggta tgccagcact ggcgatcacc gatttcacca acctttgcgg
    16561 tctggtgaag ttctacggag cgggacatgg cgcagggatt aagcctatcg tcggggcaga
    16621 ttttaatgtc cagtgcgacc tgctgggtga tgagttaacc cacctgacgg tgctggcggc
    16681 gaacaatacc ggctatcaga atctgacgtt gctgatctca aaagcgtatc agcgcgggta
    16741 cggtgcagct gggccgatca tcgatcgcga ctggcttatc gaattaaacg aagggttgat
    16801 ccttctttcc ggcgggcgca tgggcgacgt cggacgcagt cttttgcgtg gtaacagcgc
    16861 gctggtagat gagtgtgtcg cgttttatga agaacacttc ccggatcgct attttctcga
    16921 gctgatccgc accggcaggc cggatgaaga aagctatctg cacgcggcgg tggaactggc
    16981 ggaagcgcgc ggtttgcccg tcgtggcgac taacgatgtg cgctttatcg acagcagcga
    17041 ctttgacgca cacgaaatcc gcgtcgcgat ccacgacggc tttaccctcg acgatcctaa
    17101 acgcccgcgt aactattcgc cgcagcaata tatgcgtagc gaagaggaga tgtgcgagct
    17161 gtttgccgac atcccagaag cccttgccaa caccgttgag atcgccaaac gctgtaacgt
    17221 aactgtgcgt cttggtgaat acttcctgcc gcagttcccg accggggaca tgagcaccga
    17281 agattatctg gtcatgcgtg caaaagaggg cctggaagag cgtctggcat ttttattccc
    17341 tgacgaggaa gaacgtgtta agcgccgccc ggaatatgac gaacgtctgg agactgaact
    17401 tcaggttatc aaccagatgg gcttcccggg ctacttcctc atcgttatgg aatttatcca
    17461 gtggtcgaaa gataacggcg taccggtagg gccaggccgt ggctcaggtg cgggttcact
    17521 ggtggcctac gcgctgaaaa tcaccgacct cgatccgctg gaatttgacc tgctgttcga
    17581 acgtttcctt aacccggaac gtgtctccat gcctgacttc gacgttgact tctgtatgga
    17641 gaaacgcgac caggttatcg agcacgtagc ggacatgtac ggtcgtgatg cggtatcgca
    17701 gattatcacc ttcggtacga tggcggcgaa agcggtgatc cgcgacgtag gccgcgtgct
    17761 ggggcatccg tacggctttg tcgaccgtat ctcgaaactg atcccgcccg atccggggat
    17821 gacgctggcg aaagcgtttg aagccgagcc gcagctgccg gaaatctacg aagcggatga
    17881 agaagttaag gcgctgatcg acatggcgcg caaactggaa ggggtcaccc gtaacgccgg
    17941 taagcacgcc ggtggggtgg ttatcgcacc gaccaaaatt accgattttg cgccgcttta
    18001 ctgcgatgaa gagggcaaac atccggtcac ccagtttgat aaaagcgacg ttgaatacgc
    18061 cggactggtg aagttcgact tccttggttt gcgtacgctc accatcatca actgggcgct
    18121 ggagatgatc aacaagcggc gggcgaagaa tggcgagccg cctctggata tcgccgcgat
    18181 cccgctggat gacaagaaaa gcttcgacat gctgcaacgc tcggaaacca ctgcggtatt
    18241 ccagcttgaa tcgcgcggca tgaaggacct gatcaagcgt ctgcaacctg actgcttcga
    18301 agatatgatc gcactggtgg cactgttccg ccccggtccg ttgcaatcag ggatggtgga
    18361 taactttatc gaccgtaaac atggtcgcga agagatctcc tatccggacg tgcagtggca
    18421 gcatgaaagc ctgaaaccgg tactggagcc aacctacggc atcatcctgt atcaggaaca
    18481 ggtcatgcag attgcccagg tgctttctgg ttataccctc ggtggcgcgg atatgctgcg
    18541 tcgtgcgatg ggtaagaaaa agccggaaga gatggctaag cagcgttctg tatttgctga
    18601 aggtgcagaa aagaacggaa tcaacgccga actggcgatg aaaatcttcg acctggtgga
    18661 gaaattcgct ggttacggat ttaacaaatc gcactctgcg gcctatgctt tggtgtcata
    18721 tcaaacgtta tggctgaaag cgcactatcc ggcggagttt atggcggcgg taatgaccgc
    18781 cgatatggac aacaccgaga aggtggtggg cctggtggat gagtgctggc ggatggggct
    18841 gaaaatcctg ccgccggata taaactccgg tctttaccat ttccacgtca acgacgacgg
    18901 cgaaatcgtg tatggtattg gcgcgatcaa aggggtcggt gaaggtccga ttgaggcgat
    18961 catcgaagcc cgtaataaag gcggctactt ccgcgaactg tttgatctct gcgcccgaac
    19021 cgacaccaaa aagttaaacc ggcgggtgct ggaaaaactg atcatgtccg gggcgtttga
    19081 ccgtcttggg ccacaccgcg cggcgctgat gaactcgctg ggcgatgcgt taaaagcggc
    19141 agatcaacac gcgaaagcgg aagctatcgg tcaggccgat atgttcggtg tgctggccga
    19201 agagccggaa caaattgaac aatcctacgc cagctgccaa ccgtggccgg agcaggtggt
    19261 attagatggg gaacgtgaaa cgttaggtct gtacctgacg ggacacccta tcaatcagta
    19321 tttaaaagag attgagcgtt atgtcggagg cgtaaggctg aaagacatgc acccgacaga
    19381 acgtggtaaa gtcatcacgg ctgcggggct cgttgttgcc gcgcgggtta tggtcaccaa
    19441 gcgcggcaat cgtatcggta tctgcacgct ggatgaccgt tccgggcggc tggaagtgat
    19501 gttgtttact gacgccctgg ataaatacca gcaattgctg gaaaaagacc gcatacttat
    19561 cgtcagcgga caggtcagct ttgatgactt cagcggtggg cttaaaatga ccgctcgcga
    19621 agtgatggat attgacgaag cccgggaaaa atatgctcgc gggcttgcta tctcgctgac
    19681 ggacaggcaa attgatgacc agcttttaaa ccgactccgt cagtctctgg aaccccaccg
    19741 ctctgggaca attccagtac atctctacta tcagagggcg gatgcacgcg cgcggttgcg
    19801 ttttggcgcg acgtggcgtg tctctccgag cgatcgttta ttaaacgatc tccgtggcct
    19861 cattggttcg gagcaggtgg aactggagtt tgactaatac aggaatacta tgagtctgaa
    19921 tttccttgat tttgaacagc cgattgcaga gctggaagcg aaaatcgatt ctctgactgc
    19981 ggttagccgt caggatgaga aactggatat taacatcgat gaagaagtgc atcgtctgcg
    20041 tgaaaaaagc gtagaactga cacgtaaaat cttcgccgat ctcggtgcat ggcagattgc
    20101 gcaactggca cgccatccac agcgtcctta taccctggat tacgttcgcc tggcatttga
    20161 tgaatttgac gaactggctg gcgaccgcgc gtatgcagac gataaagcta tcgtcggtgg
    20221 tatcgcccgt ctcgatggtc gtccggtgat gatcattggt catcaaaaag gtcgtgaaac
    20281 caaagaaaaa attcgccgta actttggtat gccagcgcca gaaggttacc gcaaagcact
    20341 gcgtctgatg caaatggctg aacgctttaa gatgccaatc atcaccttta tcgacacccc
    20401 gggggcttat ccgggcgtgg gcgcagaaga gcgcggtcag tctgaagcca ttgcacgcaa
    20461 cctgcgtgaa atgtctcgcc ttagcgtacc ggtagtttgt acggttatcg gtgaaggtgg
    20521 ttctggcggt gcgctggcga ttggcgtggg cgataaagtg aatatgctgc aatacagcac
    20581 ctattccgtt atctcgccgg aaggttgtgc gtccattctg tggaagagcg ccgacaaagc
    20641 gccgctggcg gctgaagcga tgggtatcat cgctccgcgt ctgaaagagc tgaaactgat
    20701 cgactccatc atcccggaac cgctgggtgg tgctcaccgt aacccggaag cgatggcggc
    20761 gtctctgaaa gcgcagctgc tggcggatct ggccgatctc gacgtgttaa gcactgaaga
    20821 tttaaaaaat cgtcgttatc agcgcctgat gagctacggt tacgcgtaat tcgcaaatgt
    20881 tctgaaaagg gtcacttcgg tggccctttt ttatcgccac ggtttgagca ggctatgatt
    20941 aaggaaggat tttccaggag gaacacatga acatcattgc cattatggga ccgcatggcg
    21001 tcttttataa agatgagccc atcaaagaac tggagtcggc gctggtggcg caaggctttc
    21061 agattatctg gccacaaaac agcgttgatt tgctgaaatt tattgaacat aaccctcgaa
    21121 tttgcggcgt gatttttgac tgggatgagt acagtctcga tttatgtagc gatatcaatc
    21181 agcttaatga atatctcccg ctttatgcct tcatcaacac ccactcgacg atggatgtca
    21241 gcgtgcagga tatgcggatg gcgctctggt tttttgaata tgcgctgggg caggcagaag
    21301 atatcgccat tcgtatgcgt cagtacaccg acgaatatct tgataacatt acaccgccgt
    21361 tcacgaaagc cttgtttacc tacgtcaaag agcggaagta caccttttgt acgccgggtc
    21421 atatgggcgg caccgcatat caaaaaagcc cggttggctg tctgttttat gattttttcg
    21481 gcgggaatac tcttaaggct gatgtctcta tttcggttac cgagcttggt tcgttgctcg
    21541 accacaccgg gccacacctg gaagcggaag agtacatcgc gcggactttt ggcgcggaac
    21601 agagttatat cgttaccaac ggaacatcga cgtctaacaa aattgtgggt atgtacgccg
    21661 cgccgtccgg cagtacgctg ttgatcgacc gcaattgtca taaatcgctg gcgcatctgt
    21721 tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc
    21781 ttggtgggat cccgcgccgt gaatttactc gcgacagcat cgaagagaaa gtcgctgcta
    21841 ccacgcaagc tcaatggccg gttcatgcgg tgatcaccaa ctccacctat gatggcttgc
    21901 tctacaacac cgactggatc aaacagacgc tggatgtccc gtcgattcac ttcgattctg
    21961 cctgggtgcc gtacacccat tttcatccga tctaccaggg taaaagtggt atgagcggcg
    22021 agcgtgttgc gggaaaagtg atcttcgaaa cgcagtcgac ccacaaaatg ctggcggcgt
    22081 tatcgcaggc ttcgctgatc cacattaaag gcgagtatga cgaagaggcg tttaacgaag
    22141 cctttatgat gcataccacc acctcgccca gttatcccat tgttgcttcg gttgagacgg
    22201 cggcggcgat gctgcgtggt aatccgggca aacggctgat taaccgttcg gtggagcggg
    22261 cgctgcattt tcgcaaagag gtccagcggc tgcgggaaga gtctgacggt tggtttttcg
    22321 atatctggca accgccgcag gtggatgaag ccgaatgctg gcccgttgcg cctggcgaac
    22381 agtggcacgg ctttaacgat gcggatgccg atcatatgtt tctcgatccg gttaaagtca
    22441 ctattttgac accggggatg gacgagcagg gcaatatgag cgaggagggg atcccggcgg
    22501 cgctggtagc aaaattcctc gacgaacgag ggatcgtagt agagaaaacc ggtccttata
    22561 acctgctgtt tctctttagt attggaatcg ataaaaccaa agcaatggga ttattgcgtg
    22621 ggttgacgga atttaagcgc tcttacgatc tcaacctgcg gatcaaaaat atgctacccg
    22681 atctctatgc agaagatccc gatttctacc gcaatatgcg cattcaggat ctggcgcaag
    22741 ggatccataa gctgattcgt aaacacgatc ttcccggttt gatgttgcgg gcattcgata
    22801 ccttgccgga gatgatcatg acgccacatc aggcatggca gaggcagatt aaaggcgaag
    22861 tagaaaccat tgcgctggaa caactggttg gtagggtgtc ggcaaatatg atcctgcctt
    22921 atccaccggg cgtaccgctg ctgatgccag gagaaatgct gaccaaagag agccgcacag
    22981 tactcgattt tctactgatg ctttgttccg tagggcaaca ttaccccggt tttgaaacgg
    23041 atattcacgg cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg
    23101 cgggataact tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga
    23161 cgttatgctg ggtttaaaac aggttcacca tattgcgatt attgcgacgg attatgcggt
    23221 gagcaaagcg ttttactgcg atattcttgg tttcacgctg caaagcgaag tctatcgcga
    23281 agcgcgcgac tcgtggaaag gggatttggc gcttaatggg caatatgtga ttgagctttt
    23341 ctcatttccg ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct
    23401 ggcttttagc gttgatgata tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa
    23461 gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc ttcacgttct ttaacgatcc
    23521 ggacgggctg ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg
    23581 taacgtgccg ggcattgcta ctgtaaaatc gcaccatcat gacactcacg ctcaatagac
    23641 aacttctcac ctcacgccag attctggtgg cctttagcgg cgggcttgac tctaccgtac
    23701 tgctgcatca gttggtgcag tggcgggcgg aaaatccggg cgtcactctg cgcgctatcc
    23761 atgtgcatca cggtttaagt gccaatgccg atgcctgggt taggcattgc gagaacatct
    23821 gccaacagtg gcaggtgccg ctggtggtcg agcgcgtgca acttgcgcaa gaaggactgg
    23881 gtattgaggc ccaggcgcgg caggcgcgtt atcaggcatt tgcccgcacc ttgttgcccg
    23941 gtgaagtgct ggtcaccgcg caacatctcg acgatcaatg cgaaaccttt ctgctggcgc
    24001 taaaacgcgg tagcggccct gccgggcttt cggctatggg ggaagtttcg gagtttgccg
    24061 gaacacggct tatccgcccg ttgctcgccc gcacgcgggg agaactggtg cagtgggcgc
    24121 gtcagtatga tttacgctgg attgaagacg aaagtaatca ggatgatagc tacgatcgta
    24181 acttcctgcg cctgcgcgtg gtgccgttat tgcagcagcg ttggccgcat tttgccgaag
    24241 caacggcccg cagcgctgcg ctttgcgctg aacaagagag cctgttagat gaactgctgg
    24301 cggatgatat agcgcactgt caaaagccgc aggggacgct gcagattgcg ccaatgctgg
    24361 cgatgagtga tgcccgccgc gcggcgatta tccgccgctg gctggcaggg aagaatgcac
    24421 cgatgccttc ccgcgacgcg ttggtgagga tctggcagga agtggcgctg gcgcgggaag
    24481 atgcttcccc ctgtttacgt ctgggcgcgt ttgaaatccg ccgctatcag tcgcaactgt
    24541 ggtggattaa atccgttgcc gggcaaagcg aaaccattgt gccgtggcag acgtggctac
    24601 aaccgctgga attaccggcg ggactgggaa gtgtacagct tacagcggga ggcgatattc
    24661 gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata
    24721 ttatcgggcg caacggcgga cgtaagctga agaaaatctg gcaagagctg ggtgtgccgc
    24781 cgtggctacg tgacaccacg ccactgctgt tttatggcga aacgttgatt gcggcggcag
    24841 gggtatttgt gacgcaagaa ggtgtggctg aaggtgagaa aggcgtcagt tttgtctggc
    24901 agaaaacgct tagttaagtg aaagccggat aagacgcttc aaacgtcgca tccggcgaaa
    24961 gtcaatcagg actcgctcac caccaccgta ccgatttccg ggtggctaaa gctggtaatt
    25021 ttatccagac gcagctcgcg ggttgcacca gcggcctcga cgaccagata ctccacattt
    25081 ttgcgggaga ctaaatcgct ggccttcgcc tgcaattttt cgccatcttt cagctcaagt
    25141 gtcagcatta aatgatgctg gcaggcgagc tcaagattat cgtaatcatc acaattgatt
    25201 ggttgatacg tatcattcat tgacataatc gctcaccagt aagtttgccg cagcgtatgc
    25261 tgctttttcc ctgacagcct cagaaagggc gtcgtcggca gccatttcat tcagcacttt
    25321 caaaacgcag cccagcgcgt ccggaacgta tcctaagtct ccgctggcga tttccgcgta
    25381 ccgcttgcgt attaactcac aatatttttc cacatgccct cctgtcagca ctctgactta
    25441 accgtggatg caagtctaag cctacgaaga taaactctgt ttcgcaaggt gactatacca
    25501 cactcatttc tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga
    25561 ttcaaaagga gttttcttat ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg
    25621 gccgatctcg accgcaacca gtttctcgat gcctctctga cgctggcgcg ccacccttca
    25681 gaaacccagg agcgcatgat gctgcgcctg ctggcgtggc tgaaatatgc tgatgaacgt
    25741 ctgcaattta cccgtggttt gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat
    25801 cacctgggca ttgatttgtg gattgagctg gggctgccgg atgagcggcg gattaagaaa
    25861 gcctgcaccc aggccgcaga agttgcgctg tttacctata atagtcgggc ggcgcaaatc
    25921 tggtggcagc aaaatcagag caaatgtgtg cagtttgcca atctttccat ctggtatctg
    25981 gacgatgaac aactggcgaa agtaagcgcc tttgccgatc gtaccatgac gctgcaggca
    26041 acgattcagg atggcgtgat ctggttatcg gatgataaga ataatctgga agtgaactta
    26101 accgtctggc aacaaccttc atgattgtga tttcccgaca tgttgctatc cccgatggtg
    26161 agcttgagat caccgccatt cgtgcgcagg gcgcgggcgg gcagcatgtt aataagacct
    26221 caacggctat tcatctgcgt tttgacattc gggcgtccag cctgccagag tattacaaag
    26281 agcgtctgct cgccgccagc catcatttga tcagcagtga tggcgtgatt gtcattaagg
    26341 cacaggaata ccgcaatcag gagctcaacc gcgaagcggc gctggcccgg ctggtagcag
    26401 tgattaaaga tttaacaaca gaacaaaaag cccgacgacc cacgcggccc acccgcgcat
    26461 caaaagagcg cagactggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca
    26521 aagtgcgcag cggtcgggaa taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca
    26581 gcgatggctg taatcagcct ctttactctg atgggatgta ataatcgggc cgaagtcgat
    26641 acgctttctc cggcgcaggc tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg
    26701 ctgccgtgtg ccgattgcga agggatcgaa acctctttgt tcctcgaaaa agatggaacc
    26761 tgggtgatga atgaacgcta tcttggtgcg cgtgaagagc cttcctcttt cgcttcctac
    26821 ggtacatggg cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg tgaaaagtca
    26881 tattatcggg cgaaaggcga tgcgctggag atgctcgatc gtgaaggtaa tccgattgaa
    26941 tcgcagttca actatacgct ggaaccggcg caatccagtt tacctatgac gccgatgacc
    27001 ctgcggggca tgtattttta tatggctgat gcggcgacct tcactgattg cgcgaccgga
    27061 aaacgtttca tggtagcgaa taacgcagag ctggagcgtg gctacctgac tgcgcgcggt
    27121 catagtgaaa aaccgatgtt actgtcatta gaagggcact ttacgcttga ggctaatccg
    27181 gataccggtg cgccaaccaa agtattagcg cccgatacgg caggtaaatt ttacccgaac
    27241 caggattgca gtagtttggg gcagtaaatt gaaagccgga gcattctccg gctttcatca
    27301 ctgagtcagc ccaacaaagc ggcccgcctt gatataaatc ccaggcttca ggtgcccaac
    27361 atactgtaac tgcgtttctg gcttaaacgc agagacatcg ccagtacgca tatgcatcag
    27421 atcggcaggg ctaatccagc ccgattgcgc cagtgtaagt ggatgcccgg ctttggcaaa
    27481 tgcatcagtg acaaattccg aacaaaacca cgactttttg tctccttcgc ccacactgct
    27541 taactgcgct ttcgccaggc cgctgacgca ctgttgacga aaatcctcgg agaacggatt
    27601 cagtgagcac atctggcgag ttaccataaa gggaataaat tcgacaatgc cgcgatagtt
    27661 gtaaccgcta tctttaattt tgttggcgaa cgcggtgatt tctgtggctt gttgcggggt
    27721 aagatccggg actcgtagga cgaaaagctt atcgctatgc ttcatcgctt ttttgaggga
    27781 aacaatctgg acgccagcgc ctgtcgcttc tgcaacgtta ttttcaccca gatagattgc
    27841 aacgtgactc acagaggaag tgctgaagac gcggattcca aatgaggtta ccccatggct
    27901 tgaggagaac agcaaatcgc cgggtttgag atcaggttcc gttatttctt ttattgattg
    27961 ttcggtaaaa gagctttgat gctggaattt gactgcccat gttttcgccc cggcatctac
    28021 cgctgtggct gatgagtccg gctggctgat atctacggta caggccgaaa ggaaaagaaa
    28081 gcaggggaga agcaggcggc aatgcacctt tggtttatcc attttataca gtccatgtaa
    28141 aaaagggccc tgaaattcag gaccctttct ggcatcagcc tttaatctgt ttcaccagat
    28201 attcgacgat gtcaccagtc ttaattaact gtttctcgcc gttgcgacga tatttatatt
    28261 cgatatcgtc gttgtcgagg ttacggtcgc ccagcacaat agtgtgcgga ataccgatca
    28321 gttccatatc agcaaacatc acgcccggac gctctttgcg gtcatccagc agcacttcga
    28381 taccttgtgc acgcagttcg ctgtacagtt tctcggccag ctcctgtacg cggaaggatt
    28441 tgtgcatgtt catcggcaga atcgccacct ggaacggcgc gatagcgtca ggccatacga
    28501 tgccgcgttc gtcatagttc tgctcaattg ccgcagccac aacgcgcgtt accccgatac
    28561 cgtagcaacc catcgtcagg atttgattac ggccatcttc accctgaacg gaggctttca
    28621 gtgcttcgga gtacttggta cccagctgga agatgtgacc gacttcgata ccacgtttga
    28681 tcagcagcgt accctgacca tccgggcttg gatcgccagc caccacgttg cggatatcag
    28741 caatttctgg ggtagcgaca tcgcgatccc agttgatacc gaagtagtgt ttaccatcga
    28801 tgttagcacc agcagcgaaa tcactcatcg ccgcaacggt acggtcaatc accactggaa
    28861 tcggcatgtt taccggaccc agtgaacccg gaccggcttt aaccacggca cgaatttctt
    28921 cttcggtcgc gaaagtcagc gggcttgcaa cctgcggcag tttttctgct ttaacttcgt
    28981 tcagctcgtg atcaccgcgc accagcagcg caaccagcgg gaagctactg ccttcaaccg
    29041 ctttaaccag cagagtcttc accgttttct caatcggcag attgaactgt tcaaccagtt
    29101 ccgcgatggt tttcgcgttc ggcgtatcaa ccagcgtcat ttcctgggta gcagcagcgc
    29161 gcggttcttt cggcgcgata gcttctgcca gctcaatgtt cgctgcatag tcagaggtgt
    29221 cggagaagac cacatcgtct tcaccgctct gcgccagcac ctggaattcg tgagaggcgc
    29281 taccgccgat agaaccggtg tcggcctgta cggcgcggaa atccagcccc atgcggctga
    29341 agattttgct gtaggccgca tacattgcat cgtaggtttc ctgtaaggat tcctgagaag
    29401 tatggaaaga gtaagcatct ttcatcagga attcgcggga acgcatgacg ccgaaacgcg
    29461 gacgcacttc gtcgcggaac ttggtctgga tctgatagaa gttcagcggc agctgtttgt
    29521 aagagctcag ctcgttacga atcaggtctg taataacttc ttcatgagtt gggccgagta
    29581 cgaacggacg ctcgccacgg tcaacaaaac gcagcagttc cggaccgtac tgttcccaac
    29641 gaccactctc ttgccacaaa tcggctggct gaaccaccgg catcgacacc tcgatcgcac
    29701 cggcgttgtt catctcttca cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg
    29761 tcggcagcca ggtatataac ccggaggcca gcttgcggat catcccggcg cgcagcatca
    29821 gctgatggct gatcacctcg gcgtcggcag gtgtctcctt gagagtggag agcaggtatt
    29881 ggctagtacg catgttgtta cggttccagt tggaaggtag aacaggctca aggcgagcct
    29941 gggacaaaaa aagtgattta gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgcg
    30001 aatttaacgc ggttccagcg caaagacttc aaaacctgcg tcggtgacgc gccagcgaac
    30061 gttaaaatca tgcagccaga cggcataggt tttgcccgtt tcctcacctt tacgataggc
    30121 cgggcgcggg tcctgcgcca gtacttcgcg gataaacagc gttaactgcg gataacgttt
    30181 ctccagcgtc aaaagctgct tttcgacctc tgcggtaaag ctcaccgcca tctctgccgc
    30241 tggcgcactt tgcgcatagc tggcactggc atttggaagc gattcggcaa aggggagata
    30301 cggtttgata tccaccaccg gcgtaccatc taccagatcc agactgccga gcttcagaat
    30361 cacgctgtct ttatggcaaa caacctcttt cagctctacc agcgacatac caatcgggtt
    30421 agggcggaaa gtggagcgcg tagcgaaaac acccattctg gcattaccgc cgaggcgcgg
    30481 tggacgcaca gtcggacgcc agccgccttc catcgtttga tgaaagacga aaaggatcca
    30541 taaatggctg aacgcttcca ggccgcgaac ggcatcggcc tggttgtagg gagcaatgag
    30601 atgcagttcg ccgttggcgc tttttaccag acctggctgg cgcggaacgg cgaatttttc
    30661 tttatagggc gagcgaataa cgcctatttg ctcgaactgg aaactgctca tttcgccgta
    30721 atgttaagcg cagaaccgat acatacagcc tgacgatagc agcctggcgt accgctggtg
    30781 acttcgcagc tatgcagtaa taccgcattg gccttcattt tcgaggcgtt gatttgcatc
    30841 cgcttacgtg cggttggaat gctcggcgga gagtcctgat tggaggcctg gcaagaatcg
    30901 ccactgactt caccgagatc gcggaacggt ttgccgacta attcttctgc attggtataa
    30961 attcggaccg gcgtggcgcg cggcgctttc ggttttgcag gctccgcttt cggctggggt
    31021 gcagtgcttt gaacgggttc gacaggggat ctgcttaaca tggaacagcc gcttagcatg
    31081 agtgctacta aacagatcgg taaagcacgc atagtatttc ctcaatgtat gatcaaaacg
    31141 tcaatattga atcaggagct tgtaaaaatg acaagacggg caagcgcccg tcctgaatga
    31201 tattacaaat tgtggaaaca gcctaaaaat taccagcctt taacagcgcc gccgttaaac
    31261 actttgtttg ctgcttcgta aacttcgtca gactgataag cctggacgaa tttcttcacg
    31321 ttctcggcgt ctttgttatc ttcacgcgtc acgatcaggt ttacgtacgg ggagtcttta
    31381 tcttcgacaa agataccgtc tttcgccgga gtcaggccaa tctggctggc ataggtggta
    31441 ttgataactg ccagagcgat ttgcgcgtcg tccagagagc gaggcagctg cggtgcttcc
    31501 agctcaacaa ttttcagatt ttttgggttc tcaacaacat caagaacggt cggcagcagg
    31561 ccaacgccat ctttcagttt gatcaagccc actttttgca gcagcagcag tgaacgacca
    31621 aggttagttg ggtcgtttgg cacggcaacc tgcgaaccat cctgcaattc atccagtgat
    31681 ttgattttct tggagtaacc agcaatcgga taaacaaaag tgttgcctac tgcgaccagt
    31741 ttgtagccac gatctttcag ttgctgatca aggtacggtt tatgctggaa ggcgttggcg
    31801 tcgatatcgc ctttgctcaa tgcctcgttt ggcagaacat agtcgttgaa ggttaccagc
    31861 tcaacgtcca gaccatattt gtctttcgca actttctgcg cgacttctgc aacctgctgt
    31921 tcggcaccaa caatcacgcc gactttaatg tggtttggat ctttttcatc ctgaccgcag
    31981 cctaccaatg ccagtgatcc aatcagggct cccactgccg caaaggtttt gaatttgaac
    32041 gccatacctt attccttctt caattattta tgttgtgttg aacgttactt gcgagtgaca
    32101 gcccggacga tgcggtcgcc tgcgaactga attaaataaa ccagaatgac cagcaatacc
    32161 agtaccgtat tcatcaccgt cgcgttgtag ccgatgtagc catactgata gccaatctga
    32221 cctaaaccac cggcaccgac tgcaccaccc atcgcggaat aaccgaccag ggtaataagg
    32281 gtgatagttg ccgcattcac cagacccggc aacgcttccg gtaacagcac tttacggacg
    32341 atctgcattg gcgtggcacc cattgcgcgg gaagcttcaa ttaacccggt tgggatctcc
    32401 agcagagcgt tctcgaccat acgggcaata aacggtgctg caccaacggt taacggaaca
    32461 atcgctgcct gcaaaccaat cgatgtaccg acaataacgc gggtaaacgg aatcatccat
    32521 accagcaaga taatgaacgg gatagaacgg aaaatgttca caatcgcaga aacggtacga
    32581 tacagcttcg cgttagcaat aatttgcccc gggcgcgtaa cataaagcag gacgccgacc
    32641 ggcagaccaa tcacaaaccc aaaaaatccg gatacgaagg tcattgccag cgtttcccat
    32701 acgccacgaa ccagcagcca catcatcggc tcagacataa cccagtacct ctacttttac
    32761 atggtgttcc tgcagccagg caatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc
    32821 agtcagcatg atgccgaact taacgccacc ggcgtaatcc atctgcgcgc taataatgtt
    32881 gttgttgacg ttgaaacgac gcgcggtttc agaaagcagt ggggcatcga ccgattgacc
    32941 ggtaaactcc agacgcagca tcggcacgca gtcagtgaat ggctccgctt gcagacgctc
    33001 ctgataatct tccgggatat ccagatgcag ggtcgactga ataaacttct gcgccagcgg
    33061 cgttttcgga tgcgagaaca cttcacttac cgtgtcctgc tcgatcagtt cgccattact
    33121 gataaccgcc acgcaatcac aaatgcgctt cacaacgtcc atttcgtggg tgatcaacag
    33181 aatcgtcaaa cccagacggc ggttaatgtc tttcaacagt tcgagaatag aacgtgtcgt
    33241 tgccgggtcc agcgcgctgg tggcttcatc acacagcaat actttgggat tgctggctaa
    33301 tgcacgggca atcgccacac gttgtttctg cccaccggaa agattcgacg ggtagctatc
    33361 atgcttatcg ccaagaccaa ccaatgacag caattccgtc acgcgacgtt tgatctcgtc
    33421 tttcggtgta ttgtccagct ccagcggcag agccacgttg ccaaaaacag tacgcgaaga
    33481 gagcaggtta aaatgctgga aaatcatacc aatctggcgg cgagctttgg tcaactcgga
    33541 ttctgacagc gtggtcagtt cctgtccatc gaccagcacg ctaccctcgg ttgggcgctc
    33601 cagcaggttt acacaacgta taagcgtact cttacccgcg cctgaggcac cgataacgcc
    33661 ataaatttgt ccagctggca catgcaggct gacgttgttc aatgcctgga tggtgcgggt
    33721 gccctggtgg aacactttgg tgatattcga aagttttatc attgattatt tattatcgtc
    33781 attaagttag tcgtggcatt tcgaatgcct ggaacgggca acgccgtcaa tgaaatggat
    33841 gttaaggcat ccagacgtct aaatcaatca ggtttatgcg aagagcactt tcttgctggt
    33901 cgaaacatgc gatactagcg tcacatgcct tattaaggag ctataaaggt ggcgaagagc
    33961 gtacccgcaa tttttcttga ccgtgatggc accattaatg tcgatcacgg ctatgtccat
    34021 gagatcgaca actttgaatt tatcgacggt gttattgacg ccatgcgcga gctaaaaaaa
    34081 atgggctttg cgctggtggt agtaaccaac cagtctggca ttgctcgcgg taaatttact
    34141 gaagcacagt ttgaaacgct gaccgagtgg atggactggt cgctggcgga ccgcgatgtc
    34201 gatctggatg gtatctatta ttgcccgcat catccgcagg gtagtgttga agagtttcgc
    34261 caggtctgcg actgccgcaa accacatccg gggatgcttt tatcggcacg cgattatttg
    34321 catattgata tggccgcttc ttatatggtg ggcgataaat tagaagatat gcaggcagcg
    34381 gctgcggcga acgtgggaac aaaagtgctg gtgcgtacgg gtaaacctgt tacgcctgaa
    34441 gcagaaaacg cggcggattg ggtgttaaat agcctggcag acctgccgca agcgataaaa
    34501 aagcagcaaa aaccggcgta atgattaaaa gatgagcggt tgaaataaaa atgcattttt
    34561 ccgcttgtct tcctgagccg actccctata atgcgcctcc atcgacacgg cggatgtgaa
    34621 tcacttcaca caaacagccg gttcggttga agagaaaaat cctgaaattc agggttgact
    34681 ctgaaagagg aaagcgtaat atacgccacc tcgcgacagc a
//
LOCUS       DAOTRK010000038        34060 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.038, whole genome shotgun sequence.
ACCESSION   DAOTRK010000038 DAOTRK010000000
VERSION     DAOTRK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.038"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..914)
                     /locus_tag="QIE41_001789"
     CDS             complement(<1..914)
                     /locus_tag="QIE41_001789"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDS3530684.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVD"
     gene            complement(1125..2522)
                     /gene="ccp"
                     /locus_tag="QIE41_001790"
     CDS             complement(1125..2522)
                     /gene="ccp"
                     /locus_tag="QIE41_001790"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDS3530685.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPDHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2927..4576
                     /locus_tag="QIE41_001791"
     CDS             2927..4576
                     /locus_tag="QIE41_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDS3530686.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4627..5229)
                     /locus_tag="QIE41_001792"
     CDS             complement(4627..5229)
                     /locus_tag="QIE41_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDS3530687.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5750..6649
                     /locus_tag="QIE41_001793"
     CDS             5750..6649
                     /locus_tag="QIE41_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3530688.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGASPEDGVIARPLGK
                     LTVVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLIYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6698..7711
                     /gene="yhjD"
                     /locus_tag="QIE41_001794"
     CDS             6698..7711
                     /gene="yhjD"
                     /locus_tag="QIE41_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDS3530689.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQR"
     gene            8103..9425
                     /locus_tag="QIE41_001795"
     CDS             8103..9425
                     /locus_tag="QIE41_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDS3530690.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9607..11667)
                     /locus_tag="QIE41_001796"
     CDS             complement(9607..11667)
                     /locus_tag="QIE41_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDS3530691.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHIHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDGNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11737..12504)
                     /gene="pdeH"
                     /locus_tag="QIE41_001797"
     CDS             complement(11737..12504)
                     /gene="pdeH"
                     /locus_tag="QIE41_001797"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDS3530692.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLSPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12736..13665
                     /gene="kdgK"
                     /locus_tag="QIE41_001798"
     CDS             12736..13665
                     /gene="kdgK"
                     /locus_tag="QIE41_001798"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDS3530693.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13761..15257)
                     /locus_tag="QIE41_001799"
     CDS             complement(13761..15257)
                     /locus_tag="QIE41_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS3530694.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLSSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMTPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15477..16763)
                     /gene="dctA"
                     /locus_tag="QIE41_001800"
     CDS             complement(15477..16763)
                     /gene="dctA"
                     /locus_tag="QIE41_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDS3530695.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16946..18934)
                     /gene="hmsP"
                     /locus_tag="QIE41_001801"
     CDS             complement(16946..18934)
                     /gene="hmsP"
                     /locus_tag="QIE41_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDS3530696.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMNSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(19016..22489)
                     /gene="bcsC"
                     /locus_tag="QIE41_001802"
     CDS             complement(19016..22489)
                     /gene="bcsC"
                     /locus_tag="QIE41_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDS3530697.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQWQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNQLIPLAKSQPPSMESAMVLRDGAKFEARAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKIINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(22471..23577)
                     /gene="bcsZ"
                     /locus_tag="QIE41_001803"
     CDS             complement(22471..23577)
                     /gene="bcsZ"
                     /locus_tag="QIE41_001803"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDS3530698.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23584..25923)
                     /gene="bcsB"
                     /locus_tag="QIE41_001804"
                     /pseudo
     CDS             complement(23584..25923)
                     /gene="bcsB"
                     /locus_tag="QIE41_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            complement(25934..28552)
                     /gene="bcsA"
                     /locus_tag="QIE41_001805"
     CDS             complement(25934..28552)
                     /gene="bcsA"
                     /locus_tag="QIE41_001805"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDS3530699.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWLILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIVWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(28549..29301)
                     /gene="bcsQ"
                     /locus_tag="QIE41_001806"
     CDS             complement(28549..29301)
                     /gene="bcsQ"
                     /locus_tag="QIE41_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDS3530700.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDDQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDSSQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSKS"
     gene            complement(29313..29501)
                     /locus_tag="QIE41_001807"
     CDS             complement(29313..29501)
                     /locus_tag="QIE41_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDS3530701.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            29773..31344
                     /gene="bcsE"
                     /locus_tag="QIE41_001808"
     CDS             29773..31344
                     /gene="bcsE"
                     /locus_tag="QIE41_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDS3530702.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEGITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQNSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            31341..31532
                     /gene="bcsF"
                     /locus_tag="QIE41_001809"
     CDS             31341..31532
                     /gene="bcsF"
                     /locus_tag="QIE41_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDS3530703.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            31529..33208
                     /gene="bcsG"
                     /locus_tag="QIE41_001810"
     CDS             31529..33208
                     /gene="bcsG"
                     /locus_tag="QIE41_001810"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDS3530704.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(33295..33402)
                     /locus_tag="QIE41_001811"
     CDS             complement(33295..33402)
                     /locus_tag="QIE41_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDS3530705.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIMNWLNKRK"
     gene            complement(33778..33885)
                     /locus_tag="QIE41_001812"
     CDS             complement(33778..33885)
                     /locus_tag="QIE41_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDS3530706.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
BASE COUNT         8047 a         9310 c         8568 g         8135 t
ORIGIN      
        1 tagtcaacgt tgaacaccag ttcctgcggc agcgcttctt catcacgcca gataacccag
       61 ccgcagccca gcggagccag accgaattta tggcctgaag cactgatcga tttcacacgc
      121 ggcaggcgga agtcccagac gatatccggg gcgacgaacg gtgccaggaa gccaccgctg
      181 gcggcgtcga tgtgcatgtc gatgtcgata ccggtgtcgg cctggaattt atccaacgca
      241 tcgtgcagcg gttgcgggaa ctcatagtta ccggtgtagg tcacgccgaa agtcggcacc
      301 acgccgatgg tgttttcgtc gcaggcctca atcatgcgtt tcgggtccat aaacaactga
      361 ccggggcgca tagggatctc acgcagctcc acatcccagt agcgggcaaa tttatgccag
      421 cagatttgta ccggaccgca caccaggttt ggtttgttgg tcggcttacc tgcggcttcc
      481 atacgcttgc gccaacgcca tttcatcgcc atcccgccga gcatacaggc ctcggaagaa
      541 ccaatggtgt tggtgccaac ggcctgacca tttttcggcg caggcgcatg ccacagatcg
      601 gcaaccatgt ttacgcaacg caggtcgatg gctgcagatt gcggatattc ttctttgtcg
      661 atccagtttt tattgatcga caaatccatc aatttatgga cgttttcgtc gtcccaggtc
      721 tggcaaaaag tggccaggtt ctgacgagcg ttgccatcaa gatataattc atcattgata
      781 atctggaatg cgacatcatc gcgcatttcg tgcagcggaa atcgttttga ctccgcgata
      841 gtagaaatgg cctttgcgcc aaaacgtgaa tcgagtagtt ctgagcggaa atccgttaac
      901 agcttctggt ccatttcgaa ctccttaaat ttatttgaag gcaataaaaa agtaggattt
      961 atccgcaatg gaagcaaggc attacaggct taatttaaat aacaaaatcc taagcagaaa
     1021 aacgactttt tataatataa acatttaaca tgataatatt aaaaacagac tttattatcg
     1081 caataaaaat cacccgaaca ttcctgttcg ggcgattaaa ttaattattg tttatcctgc
     1141 atatacggcg tgtaaacccc gttcagactg tgcaggaaag ctacgatatc atccacatcc
     1201 tcctgcggca gctctttgcc tacctgatag cgcaacatca gtttcaccgc cccgtccagc
     1261 gtcggcacat caccgcgatg gaagtacggt gccgttagtg caacgttacg taaaccgggt
     1321 actttctgac gcaatttatc gcgctcttct ttagtaacat tcatacgacc aatatccgcc
     1381 gccgtaattt caccaaagtt aaagtctttt ttcagcccca acggttcgaa ggaacgtccg
     1441 ccgagaataa taccaccatg acaagttgca catttattat ctttgaataa ttgatagcct
     1501 tttttctgtt gtgccgtcag cgcattctca tctccacgca accatttatc aaatggggaa
     1561 tccggcgtaa ttaatgtttt ctcaaattca gcaatggcat cagtaatatt ttcgccactg
     1621 aaaccttgcg gatagacttc aaggaactgc gctttaagct gcggatcttt ttccagctta
     1681 gcaataattt cgtcccagga tttcgacgcc atttcaatcg gattcaacgg cggtccacca
     1741 gcctgatcct gcaatgttgc tgcacgacca tcccagaact gctcaacgtt aaatactgag
     1801 ttaaataccg tcggcgcgtt aatcggcccc actgcgccac caacaccaat cgatgttttt
     1861 ctgccatcga cgccccccgc attcaacgca tggcaatgag cgcatgaaat ggtgctatcc
     1921 gccgataaac ggggatcgtg atacagcgca aagcccaacg ccactttttg cgcatcggta
     1981 ggcagttttt gcgggatggg ctgcaccggt tcattacgat gatccggtgc agtgtcattg
     2041 ctggcgtaat attccgcgcg ctgttttgca atccaggcga gtatttccgc ccgctcttca
     2101 tcactcacct tacccgccca gtgtagcgcg gtataacgcg ttggtggcat ggtttcatac
     2161 tgcatcaccc attcaatctt attcagatcg ctttgcgaaa cgggtttatc agccagcaga
     2221 gccgcacgca cggcctcgag gttaaaagat ttatatccaa gcttaatgtc gtaatccatc
     2281 aactgtttcg cgccaggaat ataataatag gcgggtaatt ctgccgaagg cgtgtggcaa
     2341 tagtcgcatc ctttttcgcg gagaaagcct aaaaccttat tattttcact gacagcagat
     2401 gcctgaacat cagcctgttt actgcgttta ttatcgtggt accacacata accagataac
     2461 cctaaatagc aaatcgcgac gccagccagg ccgatcgcgg taatacgtga gaccattttc
     2521 attattcttc cctcacggtg gtgagttatt gtgacccaga aaattttcca gaatgctgca
     2581 aacgtctctg cgagacaaag ccatcataaa aaacagggaa atatgctttt tgataggaat
     2641 caatcaatta catgaatata aactatcaat aagatagcct gtgcctatta cccagatcag
     2701 gaatggcata tcatgattgc ggcgagagca ggattgaatg ttctgaaaat gaaaaacaac
     2761 acgaaatctg tatttttata aattccatgg atattagtgc gttatatttt ttgacaggta
     2821 aataaacgtg atccaccgca cgctttgtcg cccaccaggc ggagcgaatg actaccctta
     2881 aagaaaagcc cgataattag caacgaattt cggaggttgg atccttatgc tcaatcagaa
     2941 aattcaaaac cctaatccag acgaactgat gatcgaggtc gatctctgct atgaactgga
     3001 cccgtatgaa ttaaaactgg atgagatgat cgaggcagaa ccggaacccg agatgattga
     3061 agggctgccc gcctctgatg cgctgacgcc tgctgatcgc tatctcgaac tgttcgagca
     3121 tgttcagtcg gcgaaaattt tccccgacag taaaaccttt cccgactgcg cacctaaaat
     3181 ggacccgctg gatattttaa tccgctaccg taaagtgcgc cgtcatcgtg attttgactt
     3241 gcgcaagttt gttgaaaacc acttctggct accggaggtc tactccagcg agtatgtatc
     3301 ggacccgcaa aattccctga aagagcatat cgaccagctg tggccggtgc taacccgcga
     3361 accacaggat cacattccgt ggtcttctct gctggcgctg ccgcagtcat atattgtccc
     3421 gggcggccgt tttagcgaaa cctactattg ggattcctat ttcaccatgc tggggctggc
     3481 ggaaagtggt cgggaagatt tgctgaaatg catggccgat aacttcgcct ggatgatcga
     3541 aaactacggt cacatcccca acggcaaccg cacctattat ttgagccgct cgcaaccacc
     3601 ggtttttgcg ctgatggtgg agttgtttga agaggatggt gtacgcggtg cgcgccgcta
     3661 tctcgaccac ctgaaaatgg aatatgcctt ctggatggac ggtgcagaat cgttgatccc
     3721 taatcaggcc tatcgccatg ttgtgcggat gccggacgga tcgctgctca accgttactg
     3781 ggacgatcgc gacacaccgc gtgacgaatc ctggcttgag gacgttgaaa ccgcgaaaca
     3841 ttctggtcgc ccgcccaacg aggtgtaccg cgatttacgc gcgggggcgg cctccggttg
     3901 ggattactct tcccgctggc tgcgtgatac tggtcgtctg gcgagcattc gtaccaccca
     3961 gttcatcccc atcgatctga atgccttcct gtttaaactg gagagcgcca tcgccaacat
     4021 ctcggcgctg aaaggcgaga aagagacaga agcactgttc cgccagaagg ccagtgcccg
     4081 tcgcgatgcg gtaaaccgtt acctctggga tgatgaaaac ggtatctacc gcgattacga
     4141 ctggcgacgc gaacaactgg cgctgttttc cgctgccgcc attgtgccgc tctatgtcgg
     4201 tatggcgaac catgaacagg ccgatcgtct ggcaaacgcc gtgcgcagtc ggttactgac
     4261 acctggcggg attctggcaa gcgagtacga aaccggtgaa cagtgggata aacctaacgg
     4321 ctgggcacca ttacaatgga tggcgattca gggatttaaa atgtacggcg atgaccttct
     4381 gggtgatgaa atcgcgcgca gctggctgaa gacggtgaat cagttctatc tggaacagca
     4441 caaactgatc gagaaatacc atattgccga tggtgttccc cgcgaaggcg gcggtggcga
     4501 gtatccgttg caggatgggt ttggctggac taacggtgtg gtgcgccgtt taattggttt
     4561 gtacggtgaa ccataatatt tttacagcca gccgctaact tcctgctggc tgtaaaatta
     4621 tcctcttcag gaggagatat ttaacatcat tgccgcctgg gtgcgatttt tcacttccag
     4681 acggcgatac agggattcca gatgcgcttt taccgttccg gtactgatat tcagcgccct
     4741 gccgatctcc ttatttgatt cgcccgccgc taacatggtt aaaatctccc gctggcgggc
     4801 gcttaacgat ttgagatctt taatgtcctt ttccggcgtc gtccgccagt ctccaggcag
     4861 aaacatcatc cccatcgccg cactatttac cgccaacgca aatgtctcga cggttgaatc
     4921 acgaggcaca atggccagca cattaaaatg gataacttcc tgtaaccacc gtttattgca
     4981 atccgtcgcc gtaattaaca ccttaacctc aggaaattgc accacggttt tttgcagtaa
     5041 ccagtagcaa aactcaccat cctgatcgcc atcgagcatc actaaggctt cagggtaact
     5101 ttccagcttt tgccataact catctgcctg actggccccc tgaatactca ctcctggaat
     5161 acgctgctgt aaactgattt tcattccatg aataaatatt gactgcctgt caaacatgac
     5221 tatttgcata actgaatctc cacctgaata cgttaaaaag acttaagtag tggaagggta
     5281 ttacccgcga gaaaaaataa gaattcgcca tttggcggtg gccattctac agagatgacg
     5341 cgtagaaaat agttaccgat ataaatcgtt acagctaaac gcctgaaatt acatgtcgag
     5401 ggcactattt aaaacaattt tgaggatttc cttatattga tggttagtac gcatgcaatt
     5461 aaaaatgaaa ttccgcgacc acaagcaaaa ataacaaatg gcaatgagac aaaaataagc
     5521 acaaatagcc aacacgtcct ctgttcactt taaagggaat cgctgaaaaa aaacgctctg
     5581 tttagggaaa ttcacctttc tcataaagct attccgccct tttcctgctg agaaatcgcc
     5641 acattcgtca tgacaacatt gtgaaacccg gcattagatg ttagaaaaaa caataaacaa
     5701 tgcgatatgc gcgttactgt ttcgctgtga acaacaatcg gtcaaagaaa tggataaaat
     5761 tcacgcaatg cagttgttca tcaaagtcgc ggagctggaa agtttttccc gcgcggcgga
     5821 tttctttgct ttgccaaagg gaagtgtttc gcgccagata caggcactgg aacatcaact
     5881 tggcacccag cttctccagc gcaccacgcg gcgggtcaaa ctcacgccag aaggcatgac
     5941 ctattatcaa cgggcaaaag atgtgttgag taatctcaac gaactggacg gtctgtttca
     6001 acaggatgcc accagtatca gcggtaaatt acgcatcgac atcccgccag gaatcgcgaa
     6061 aagcctgtta ctgccgcgtc tgtcggagtt tctctatctg catccgggaa ttgagctgga
     6121 gctgagtagc catgaccgtc cggtagatat tcttcacgat ggttttgatt gcgtgatacg
     6181 cactggcgcg tcaccggaag atggcgttat cgcccgtccc ctcggcaaac tgaccgtggt
     6241 caattgtgcc agtccacact atctgacgcg ctttggttat cctcaaagcc ccgacgatct
     6301 gacttcgcac gcaatagtgc gttacacacc gcacctgggt gtacatccgt taggttttga
     6361 ggttgccagc gttaatggcg tccagtggtt taagtctggc ggcatgttga cggtaaacag
     6421 tcgcgaaaac tatctcgccg ccggtcttgc cggtctgggg attattcaga tcccgcgcat
     6481 tgccgtgcgc gaagccctgc gtgccgggag gcttattgaa gtattacctg gctaccgtgc
     6541 cgagccgctc tccctttcgc tgatttatcc gcagcgtcgg gagctttccc ggcgtgtaaa
     6601 cctgtttatg cagtggctgg ctggcgtaat gaaagagcac ctggactgac cgactatact
     6661 ttttaagaat gaccacgaca gaaggacaaa agagcggatg acgcaggaaa acgagatcaa
     6721 acgtcccacc caggatctgg agcacgagcc gattaagcag ttggataata gcgagaaagg
     6781 cggcaaagtt agccaggcgc tggaaaccgt caccactact gccgagaaag tccaacgcca
     6841 accggttatt gcgcacctga ttcgcgcaac agagcgcttt aacgatcggc tgggtaacca
     6901 gtttggtgcg gctatcacct atttctcatt tttgtcgatg atcccgattt tgatggtgtc
     6961 gtttgccgca gggggctttg tgctggcctc ccatccgatg ttgctacagg atatcttcga
     7021 caaaattctg caaaacatca gcgatccgac gctagccgcc acgttgaaaa acaccatcaa
     7081 caccgccgtt cagcagcgta ctaccgtagg gcttgtcggc ctggcggtgg cgctttattc
     7141 cggcatcaac tggatgggta acctgcgtga agcgattcgc gcccagtcgc gcgatgtctg
     7201 ggaacgctcg ccgcaagatc aggagaagtt ctgggtaaaa tatctgcgtg attttatttc
     7261 gctgattggt ttgttgattg cactgattgt gacgctttcg atcacctcgg ttgccggttc
     7321 ggcgcagcaa atgattatta gcgccctgca cctgaacagc attgagtggc tgaaaccgac
     7381 gtggcggttg attggcctgg cgatttccat cttcgccaac tatctgcttt tcttttggat
     7441 cttctggcgg ttgccgcgcc accgtccgcg caaaaaagcc ctgattcgtg gaacatttct
     7501 ggctgcgatt ggttttgaag tgattaaaat cgtgatgacc tacaccctgc catcgctgat
     7561 gaaatccccc tctggcgcag catttggttc cgtgctgggg ctgatggcgt ttttctactt
     7621 cttcgcccgt ttgacgctgt tttgcgcggc gtggattgcc accgccgaat ataaagacga
     7681 cccgcgaatg ccggggaaaa cgcagcggta attcattggt ttataatgcc tgataaacgc
     7741 acggtcgatc ccctcgcccc ttcggggaga ggattagggt gagggggtac aagccagcca
     7801 gagaccaggc aacgacatgc acataaccac ctgaaactac atctcttcag gagcaaacaa
     7861 tttcatacca actcataacc ccagcatata aatccagttg gtaactttta tttaacctga
     7921 aaccagtttt atccactatt tataaaatta tgtgaagcat ttcatagaag aaaaatcact
     7981 ggcctaaaca ttatcccctt tttgcctggt ttttgaccat ttccgcgatt tgttacacat
     8041 tgaaatatca cttttgctgt gcgtaatatg gctattcgtt agccaaaaaa taagaaaaga
     8101 ttatgcaagc aacagccaca acactcgacc acgagcaaga atacacgccg atcaactcgc
     8161 gtaataaagt ccttgtcgcc tctctcattg gcacggccat tgagtttttc gacttttaca
     8221 tttacgccac tgcggccgtt attgtgtttc cgcatatctt cttcccgcag ggcgatccta
     8281 cggcagcaac gctccagtcg ctcgccacct tcgccatcgc cttcgtcgcg cgccccattg
     8341 gctctgccgt ttttggtcat tttggcgatc gcgttgggcg taaagcgacg ctggtcgcct
     8401 cgttgctaac gatggggatt tcgaccgtgg tgattggtct gctgccgggc tatgccacga
     8461 ttggtatttt cgccccgctg ctgctggcgc tggctcgatt tggtcagggt ctgggcttag
     8521 gcggtgaatg gggcggcgcg gcgctgctgg cgactgaaaa cgccccaccg cgcaaacgtg
     8581 cactgtatgg ctccttcccg cagctgggcg caccgattgg cttcttcttt gccaacggca
     8641 ctttcttgct gctttcctgg ctactgaccg acgagcagtt tatgagctgg ggctggcgtg
     8701 tgccgtttat cttctcggcg gtgctggtca ttatcggcct gtatgttcgc gtgtcgctgc
     8761 atgagtcgcc ggtgtttgag aaagtcgcta aagcgaaaaa gcaggtgaag atcccgctgg
     8821 gtacgctgct gaccaaacac gtacgcgtca cggtgctcgg taccttcatc atgctggcaa
     8881 cctatacgct gttttacatc atgacggtct actctatgac ctttagtacc gccgccgcgc
     8941 cagttgggct tggcctgccg cgtaacgaag tgttgtggat gctgatgatg gcagtgattg
     9001 gttttggcgt gatggtacca gtcgccggat tactggctga tgcctttggt cgccgtaaaa
     9061 gcatggtaat catcaccacg ctgatcatcc tgttcgcgct gttcgccttt aacccgctgc
     9121 tcggttctgg caacccgatt ctggtttttg ccttcctgct gctggggtta agtctgatgg
     9181 ggctgacctt cgggccgatg ggcgcgctgt taccagagct gttcccgaca gaagtgcgtt
     9241 acaccggagc atcattctct tacaacgtag cgtcgattct cggggcttcc gttgcgccgt
     9301 atatcgcagc ctggttgcag actaactacg ggttaggtgc ggtggggtta tatctggcgg
     9361 cgatggctgg cttgacctta atcgccctac tgctgaccca tgagacgcga catcaatcgt
     9421 tgtaataaga gtgaaaagcc tgatgcgcta cgcttatcag gcctgaattt ccatacaata
     9481 tattgaattc gcaaagattt gtaggacgga taaggcgttc acgccgcatc cggcatgaac
     9541 aaagcacact ttgccaacac tctaaatctg tttaattgcc ggatgtcaga catccggcaa
     9601 ttatcatcac ttcttcatct gcgacaaaat tgtccggcac tgattagcct ccccttcgga
     9661 aggtgagatc agcgccagta acgccgccgc tggcgtaacc agcgttgcca gtgccgccgc
     9721 aacagcacca cgggcaatca acggcccggc tttcacccca gcctgcggat ttttaaatgt
     9781 cccacgcaca tacagcggcg aacgcagcgt gataatccga atccctttac tttctggatc
     9841 gatagtcaaa tccagttgct ccgaagcaaa acttgccgtg ccggtaacat tgatcaacgc
     9901 attctcagta tcaaaagcaa aaatctgcgg acgcgccacg ccgttggcaa tatccagatt
     9961 cgccgccgca cagttcaccc gcacctcatc gtcaccaaat atcgcaccga caatgtagtt
    10021 accgacattc agcccgacaa tctccatcag gttgcggctc accagcccgt cattcatcaa
    10081 cagtttcagg ttgccgttac tgttgcctaa aagcgccgcc accgagttac cgctaccgcg
    10141 tagttccgcg tcaccgttca tttcccccag cgtcttctgc atcagttcta catcgggcat
    10201 cagttctttc agtttcagcc gacgagcctg aatatctgcc cgcccttgca tcggtttttt
    10261 atcaccttcc agatgaatat tcgccgcaat gctgccgccc gccatgccaa atttcagcgg
    10321 ttgcaggcgc aggtcagcat ttttgaggat gatatgagta gaaagatcgc taatcggcag
    10381 gctactgcca tgctcaatgc gccgcccttt gaagcgaaca tcggcatcca tgacgtccca
    10441 tttatcggtt tcgaagcggt cataaggcag cactttgccc gcaggctgaa cgcttttttc
    10501 acccttcttc tgttcagacc gtttcgactt ttcagctcct ttcccggaat caacgccaat
    10561 caacggtccc aagtccgcca gccgcaattg ccgcgactcg acatcacctt ccagttttgg
    10621 tcgtggcttg ccggtggtgt agaccagaga accgtggata tcgctatcgc caatgcgccc
    10681 attaaaaccg cgataatcaa agaccgacga tttttcagtg tcgattttcg ctaccagccg
    10741 accatccgtt tcaaacggcg gggtatcggg cagcagaacg cccgtcagtt catagagatc
    10801 acccagcgaa tcgccagaaa atttaagccg taaatcgaca ccacccatct tcattgggtc
    10861 attcacaacg ccatcaaaag caacacgggt gttaccagag cgaaaatcag cctgtaccgg
    10921 aaatggtgta ccttcgccac gcagcgccag cataccgcct atttttcccg taccagtgag
    10981 cggttcaccg ttataacgtc cctgcgcctt caggccaaaa acgtaatcac ccaccttttc
    11041 tttatccgct ttacctttcg atccagtaac ttcgctgaac ggcagcggct tgcctaacgg
    11101 atcgacaaaa atttccagat ccgctttgct tactttgtca tcaatggcga tccgcccttg
    11161 atcgaaaaga atattatcca gccgaaacga ccatgccgac ggctttgcat tcgcgtcttt
    11221 gttgccatcg ttggcaagat taaacgtcca gttattgttc ttttcagaga ggcgaatcag
    11281 gcgtgcgtcg ggcttttcca gtttgatcca cggtagccag accgttttgg tcagcagcgc
    11341 cagcggggcc agcgttgcct ctacgcgtgg caaatgcacc attgtgactt cgggaatatc
    11401 tggtgggttg ccgagaatga tgtcttccgc gtgtatatgg ggccacggca cccagctgcg
    11461 ccagccagtt tcttgttttt gtcgctccca caccacgccc agatcgccac ggatagcgaa
    11521 cggacgattc aactccgcag agactttctg gttgatggtc ggtttgagtc gattccagtc
    11581 aaaagttgca atcaaaatga tcgccacgac aatcaacaac aagaaagccc ctgaaatcgc
    11641 agcggttatt ttgcctgcct tgctcatcgt tcgcttcctc atattcttcc tgtgccagtc
    11701 ctaaagatag tccagccagt cggaaaatga ggcagcttat agcgccagaa ccgccgtatt
    11761 cagcgtttct atcggtgccg ggcgtgaaag aaaccagcct tgtgcggcga atgcgggcga
    11821 gttctgtaca tcgcgccact cttccggcgt ttctacgccc tcgacgatca ccccgcgaca
    11881 atagcgattc attagatgta aaagctgaga aaagagtgtg cggccttccg gcgactgacg
    11941 cagcatcaca aacagttctc gcgcgatttt gatgtagtca taacgcactt cacttagtgc
    12001 agagaaattt gccatcccgg tgccaaaatc atccagccac agcgggccaa attcacacat
    12061 cgaggcaaag gttgaatctt tcggcagacg gatatgctcc accagttcga aacgcagcca
    12121 gggaagacgc tcaatctggc gcaggatttt tggttgctga cgcagggcga tgagcgtagg
    12181 tccatcaata ttgaccgatg ccagcaggcc gtgctctata aagaagtcgg ctttttgcgc
    12241 cagcaaatca atctgctctt tcacaacctc catccgatgg ctgacggtga tttcagtaaa
    12301 atagcgatcc ggcgacaggc gttgcgaagg gttcaaggga tgcgtgacca ccgttaatag
    12361 ctccacggcc attaaccgcc cgcatgtttg atagatcggc tgccaggtgt aagcacgctc
    12421 acactgcaac caaaaacgcc gttcctgcaa gctctcgatg cttgcttcag ggttgcttat
    12481 tcgctggata acctgcctta tcatcaaaga tgtcctgtgt ttaaaagtga tgctgcccgg
    12541 actcgacaaa gattatcggc gcgcaagggc agaactttac cgcctgaccg gagtaaattt
    12601 ccccactcca caacggaatc tttcaggaat acgtgctggc tcaaaaaaat aatggaacac
    12661 tgttttaata tggttgacca gcaaaccacc acagcgcaaa ctaacgttaa ttttttacag
    12721 atcaggttca cgactatgtc caaaaagatt gccgtgattg gcgaatgcat gattgagctt
    12781 tccgagaaag gcgcggacgt taagcgcggt ttcggcggcg ataccctgaa cacttccgtc
    12841 tatatcgccc gtcaggtcga tcctgcggca ttaaccgttc attacgtaac ggcgctggga
    12901 acggacagtt ttagccagca gatgctggac gcctggcacg gcgagaacgt tgatacttcc
    12961 ctgacccaac ggatggaaaa ccgtctgccg ggcctttact acattgaaac cgacagcacc
    13021 ggcgaacgta cgttctacta ctggcgtaac gaagccgccg ccaaattctg gctggagagc
    13081 gagcaatctg cggcgatttg cgaagagctg gcgaatttcg attatctcta cctgagcggg
    13141 attagcctgg cgatcttaag cccgaccagc cgcgagaaac tgctttcttt gttgcgcgaa
    13201 tgccgcgcca acggcggaaa agtgattttc gacaataact atcgtccgcg cctgtgggcc
    13261 agcaaagaag agacacagca ggtgtaccag caaatgctgg aatgcacgga tatcgccttc
    13321 ctgacgctgg acgacgaaga cgcgctgtgg ggtcaacagc cggtggaaga cgtcattgcg
    13381 cgcacccata acgcgggcgt gaaagaagtg gtggtgaaac gcggggcgga ttcttgcctg
    13441 gtgtccattg ctggcgaagg gttagtggat gttccggcgg tgaaactgcc gaaagaaaaa
    13501 gtgatcgata ccaccgcagc tggcgactct ttcagtgccg gttatctggc ggtacgtttg
    13561 acgggcggca gtgcggaaaa cgcggcgaaa cgcgggcacc tgaccgcaag taccgttatt
    13621 cagtatcgcg gcgcgattat cccgcgtgag gcgatgccgg cgtaaaagca gcatatgcaa
    13681 aaggctggat aaagtgttac ccctctatcc agctcatcca taaatacctg atggttgtgc
    13741 taactcatca ccatcaggca ttactgtgcg ggtggaatat ccgtcacttc aggatgaacg
    13801 tcatccgtgg cgacagaggt ggtcgcagcg gcagtagatg gggtcatgat ttgatcccag
    13861 gccgcctgca acgctttcat gttaaattcc ggctcgcctt tcggctgcag cagtattaac
    13921 gccatatcgt tcgacaactg ctgacgcaga tcctgattta acatctccac cgtcaggctg
    13981 ttaaggaagt cctggcgcag tttctgatat tgctccggcg cgatatcgac aacctgattt
    14041 tgcaacgaac gcatccgctg ccccatcaga atatcggtat cagctcgcgc ataggcggca
    14101 aacagtttct gcagctccag tttcttttgc gccactaacg cattgaactc ttcttccggc
    14161 agacctttat cgcgaacctt cgccagttca cgcgccacca gattaaggtt gctgctcagc
    14221 ttgtcgtttg gtgattcgat gttaatggca cactgcgcac gcagatacag cacacgacag
    14281 tcaaatccaa gaccgatatc tttgctgtta ctggcgctta acgcttgctg aacatgccag
    14341 aacagcgcct cacgggccag gtccgcacgc cagtagcgca gcagtgcggc tgattcacga
    14401 atcggctgcc acggcgtatc ccacatgatt gataaccggt cctgacgcac cgcgtcagtc
    14461 ataatactca ccgcttccgc acgcagcgga gaaagcgtcg gcaccggagc cggtgtttca
    14521 cgtttgcctt tcagttcgcc aaacgtttta ttgatttggt cgacgaccga gcgcgcatcc
    14581 acgtttccca ccaccagcag cgtcattgca tccggggtat accatttctg atagaaatct
    14641 ttaatctttt ccgcttcaac gggttgtttc agcggatcgg caggatcgtg acctaacaag
    14701 gttgatcctt tcagacgata gcgccaccag ccctctttag tatcggcagg ccaggttgcc
    14761 accatgtcct gactttgcag cgcgtggttg atggtttctg gcgtgatagt cagtttgcca
    14821 gtggcatttg ccagataaga gagcgcttct ttcagcaagt cgttacggtt attgggcaaa
    14881 ctcagattaa acagcgtggt gtcataagag acaattaccg gcggcatcgg gcgtttaggg
    14941 tcgatcccct gctgccacaa tgaacgcgcc tgtgctgctt caaggccacc gctttgcgtt
    15001 aacgcaatac gagggatggc gtggctgtaa ccgctctgtt gtgtactttc ggcgagcgaa
    15061 ccggtattaa ccagcaggcg aatttcaaca cgatcgctgg gacgctgggg cgtggtcagc
    15121 acttgccact gtaaaccgtt ggaaagcgtc ccctgttgcc atgctggatc aggctggagc
    15181 gcatctgcct gcacatagcc agcagtggcc atcatcagca aaccgcccgc taaaagtcga
    15241 atttttgtgc cctgcatgtg aacccctgat caactatcct ggtaataaaa aactgcccgt
    15301 tgtcggtcag tctaaataac gttaaaaaca cttcgtgtta gaccgcaaga acatgaaaat
    15361 gtcacggaag aagtgaaata aacctgaact cacccaggtg gtaagttcag gcacaggggt
    15421 caattatgcg caaacacccg cactcgggga agggagtgcg ggcataagtg gtgagattaa
    15481 gaggataatt cgtgcgtttt gccatccggc gcacgattat tcagcacatc gtccagtttt
    15541 ttgtggtcca gttctttcac ccacttagca acgacaatgg tcgctacgcc gttaccgacc
    15601 aggttagtca gcgcacgagc ttctgacata aagcggtcaa taccgaggat cagcgccaga
    15661 cccgctaccg gcaaatggcc caccgcagag agcgttgccg ccagcacgat aaaaccacta
    15721 cccgttaccc ctgccgcccc tttagaagaa agcagcaaca cgattaacag cgtgatttgg
    15781 tggacgatat ccatctgact gttagtggcc tgggcgataa acaccgccgc cattgtcagg
    15841 tatatcgatg tgccatcaag gttaaacgag tagcctgtcg ggatgaccag ccccaccacc
    15901 gatttgcggc agccgagttt ctccatcttg tcgagcatac gtggcagcgc cgactcggaa
    15961 gatgaagtcc ccagtacaat cagcagttct tcacggatgt agcggataaa tttgaagata
    16021 ctgaaacccg tcgctttagc gattgaaccc agtaccagca ccacaaacag gatacaggtg
    16081 atgtagaagc agataatcag ctgccccagt tgcaccagtg tgccgacgcc gtatttaccg
    16141 atggtaaacg ccattgcccc gaacgcacca ataggtgcca gacgcatgat catattgatg
    16201 atgccgaaga tgacctgcga gaaactttcg atcacgttaa aaatcagttg gcctttgctg
    16261 cccagacggt ggagcgcaaa accaaacagt acggcaaaca gcagcacctg cagaatgtta
    16321 ccgctggcaa atgcgccaat gacgctcgcc gggatgacat ccataatgaa ggcgacaatg
    16381 ccctggtctt tcgcctgatc ggcgtaaacc gctaccgctt tcgcatcaag cgttgccgga
    16441 tcgacgttca ttccggcacc aggctgcacg acgttaacga tgataagacc aataatcagc
    16501 gcgatggtac tgacaatttc aaagtaaagc agtgcgactg cgccggtacg accgaccgcc
    16561 ttcatgcttt ccatgcccgc aatgcccgtt acgacggtac aaaagatgac aggagcgatg
    16621 atcatcttaa tgagcttaac gaacccgtcg ccaagcggtt tcatttgctc gcctatttca
    16681 gggtagaaat ggccaaggag aataccaatg gctatcgctg tcaggacctg aaagtaaagg
    16741 cttttaaaca gagaggtttt catagggtgt cctttagtaa aaccacaggt cttgtaaggt
    16801 tatggggtac ctgcggcctt aaaataacac ccagacaaca tcacagaaat gtacctggat
    16861 cataattgaa acaaaaaggt taaaaagttt gagctggctc gcacaaacca gcacttttta
    16921 aagttttgta atcagtttgg ggtagctact tttcttccag gtaactctct tcgaagattt
    16981 caatagggag tgggcgagca aaaaggaagc cctgggcaat accaacgccc gcttttgcca
    17041 gccagtcgcg ttgtgcttca gtctccacgc cttcggcaat catttgtaag ttcaggcttt
    17101 gcgccaacat gataattgca gcaatcatgc tgctatcttc cggcaagcct tcaacaaaca
    17161 ttttgtcgat ttttaacacg tcgattggca acgatttcat atgctgcagc tgacgcagcc
    17221 ctgcgtagcc catgccgaaa tcatccagcg ccacccgaac tccggcattg cgcagcggac
    17281 ggaggattgc caccgcagca tgagggtcgt caatacgtcg gctttctgtc acttccagaa
    17341 tcagtgttcc cggctgaatg cgatagcggg ttaacagttc cagcatatcc gccaccatat
    17401 tcgggtgcat cagttgcagc gcagagaggt ttaccgacaa gggcagcata atgccgcgct
    17461 cttgccaggc tgcaagcaat cgacaggact cttccagcac ccagtgaccg acggtaacca
    17521 tcagcccaca gcactcaatg cgatcgatta agccatccgg cagatcccaa ctgccatccg
    17581 gttgctggat acgcagtaac acttccgcac tgaccagttt accgctgttc atctcgacct
    17641 gtggctgtaa ccaaatcgca aactgatgat tttccagcgc attaaggata tcgctctctt
    17701 ccgtcaaccg ctgctgggcg gcttccatct gctgcggatc aaagaactga atctgattct
    17761 tgcctttatg gcgagcggta aatgccgcag aaatagcgcg actgtaaagc tgttcggcgg
    17821 tgagatcgcc gtagaacatc gccacgccaa tgctacagtg cggacggagt tgaatacgtt
    17881 caatcggcag gcgctcgctc atgatagtga gcacttgctg acctaaagtg attgcgtgcc
    17941 acggttcctg tacaccattg gcaatgacag caaagtcata accactaatc tgcgcgagga
    18001 tcatacgtgg cgacagtacc gatttgagtt tttccaccag cgtcagcagc agaatttctc
    18061 gttgcgcctc tttcagcacg cccgcagtat cacgcagggt ttcacaggtg atgatcatca
    18121 gcgcggtggt ttgtttacgc gcgacaacct gctccagcat ctccatcagc aaggctttgt
    18181 tcggcaaatc cgacaccggg aagcgcatcg cgttttcgtt ctgttcttca taatggcgct
    18241 gcagcaattg ctggttgagg ttgtaactgc gcaccaacat accgatttca tcatcctgat
    18301 gcagacgcgg taatgccagt tggtgaccaa caagctcctg ggctgggatg gcgttaagtt
    18361 cgcgagcaat attgcgtaaa ggatgtaaaa tcagtcgatt aatacaccag ctgatggcga
    18421 cggtcaggat aagcgacaaa agtaagtaaa tggtcactaa cgttgagagg gtgctcatca
    18481 cgaacttata catgcggaag gaatccgcct gtagcaccag ataggcaatt ggctgcgggt
    18541 ttgccggacg ttccagcgag taaacgccca gcgagatttg caccggtagc tcaaacaggc
    18601 gagtaaccat taccggcacc ggacgctctg gaataaaact tttacgcagt gcctggaact
    18661 ggttgggcag caccacatcg gcacggctga ccacgcctgc cggtttaatg ctggcaagaa
    18721 tagcttccgc ttcgggaata tcgcctttca aaatagctga agataagggt tcgcggacag
    18781 agcgagcaat gctttccagt tgcgtagccg tgttatagcg attctgctgg accagatgga
    18841 acagcaaaac ggtgcaaaaa ataaaaacga acaccaggac aacggctgcc accattgcca
    18901 tctgcttgat tgttaacgag cgacttacgc gcaaattgtc tccccccgga aatcccagat
    18961 tgccgcccga gtatacccga tcgcagcggc attaagagag gcgctatctg aaaacttacc
    19021 agtcggcgta aggtatcagc ggctgcggcg gtaaatccat gtcaccctgc catccggcgg
    19081 cggaataacg tacgtagagc aggaaatggc tgggtgcgta atctttcgcc tgctggatat
    19141 caattgccgt gccgacaaac cagttggaag taacacgtcg ttcaagtaat gcccgcgccg
    19201 tgtagccaaa gccctgactg ctgccgccat cgttcgattg ccgcgcagct tcttcctgcc
    19261 agtcggtcgg gatcagattc atcagcggat aacgcggcat ggttttggtg cgtgaatgcg
    19321 accacgagcc agacgccccc agctcccacg accagttttc cgtgcgctcc cgccacatca
    19381 ccggtatggc aaacgacagg tattcctgcg ggctgtagta accgccctga ccgagtgagt
    19441 agccactcag gtctttgtcg taatgccaga tcatattgtt aagaccaatg gtcacacggc
    19501 gattattctg gttgatgatc ttatagtaat agcccgtcat ccagcgcacg cgccagttat
    19561 cttcgacatt tttaccggtt aactggtcac cgctaagcga tgcccagacg ccgtttgctt
    19621 cacctttatc gtagctcagg cttagcccca cgccgtcggc acgcacgccg ccccattttt
    19681 tcccggtatt gctcggggag tctttttgcc cgccaaaggc cagcaaagaa ctggagatgg
    19741 gccgacgatg ggcgttaacg gtgtaaccca gcggcccgat atcatcgctg taactgatgc
    19801 cgccgaccac atccaccaca ttgaagccca tcggcgtggt gccgatatcc cagctccaga
    19861 cgtcatttcg ccagccgacc gccacgctgg caccggaatc cgactggctg cggttgccgc
    19921 tacagtcttg taatgtacag gtgccccagt tgtcatccca tttgccatcg gcattagtgg
    19981 agaaactgcc gacgttcata ttgacgaaat cactgcgaaa gaacatccgc ccgtcagaat
    20041 acggcgcatc cacctgcaac atggtagtgt gcgctttcag atcggagtaa ccaccggtgc
    20101 cgctcgatcc ccaataatcg tgctcaaggg tgacgttaag atcctgctgg cgatagaggt
    20161 ccgctgcatc gctgcgcacg ccgcgtttca gccagtcatc tttctcgtcg ttacgggtca
    20221 gccgggtaaa ggtgtcgtta tcctgcggac gcgtcgtggt cacaccggat gcgaccatag
    20281 cgtctttgta ggtttccagc gcctgctttg gatcgcccgc ccgcgcttca aacttcgcac
    20341 cgtcgcgcag caccatcgca ctttccatcg acggcggctg agattttgcc agcgggatca
    20401 actgattaaa cgtctgctgc gccgctgcgg tatcgccaag ctgcgcctgt gccagcgcca
    20461 cgcgccgctg tgtgttcagc gaggcgttat cggtagcggg cagtttcgcc agctggctac
    20521 gtgccgccgc tttgtcaccg gcagcaatat ccacttccgt cagaccaaga atggcatcgg
    20581 cgttagttgg ctcccgcgtc aggacattct gatatgcagc gcgagcggcg gtgtaatcac
    20641 gtcgttgttg cgcccagtca gccagcgtga ggtctatgcg cgtggaaggt ggttgctggc
    20701 gcagcatcgc ttccgcttca gcttctttgc cgctttctcg caggcgatta gcggtttcca
    20761 gtacctgatc gctttgcagt cgattaacca gctcctgaat attgctgctc cactgcgcgc
    20821 gcggcaggct gttgatatgc gccagcgccg ctctgtcctg gtcatgacca gagagataca
    20881 gcccgtaagc gtaaacctgc tccgggtcgt ttggcttctg ctgtgccaga ttgcgcatta
    20941 atgtatcagc ctggctgcgt tgtccggcct gccagagatc ctgcgaaagt cggtaagtaa
    21001 tccatacgct gccgggatcc agcgccagtc gttgccactg aagtgctgcc gcctgcgccc
    21061 atttgccctg gttttccagt gcctctgcct gctgtgccag acggtcgttt tgcaggctgc
    21121 gttcgatatc atcaatgctt cgccgctgac tggcagagag cgaggcgata aacgcttcag
    21181 ctttttctgg cgattgctgg cggtatatat tcgccagccc gcgcacggcg ttagtgttgc
    21241 cgctgtccat acgcaaggtc tgctgataat aacgttcggc ggcgggataa tcttttcgcg
    21301 ccatcgccac atcgcccagc cccagcactg catagctgtc ggtgttatcg acattacgcg
    21361 cctgctggaa caggcgttct gcccggtcag gattattggc tttcagcgca gcatcgccct
    21421 gctggatcgc cagccagtag cggtttactt tcaggagact gttccatttg tcgttgttgc
    21481 tgctgtgcgg atccagggcg agggcttttt ccagattcgc caccgcattg gcgcgatcgc
    21541 ctttctgaga atacgcctgg cccagcgccc ccagagcctc actgtctttc gggttcgccc
    21601 gcactgcctg ttgtagttcg ggaatggctt tacccgccat accagagtcc accgccgcta
    21661 aaccttgcgc acgagcgcgg aaagcagggt cggccagctg tttttgctgt tctgccagtt
    21721 gcgattgcgc cgcagctacg ctatcgccat cactaaagat cgagagatat tttttcagcg
    21781 ccgacacact ggcatcgctg acgggcatgt ctttaatctg cccgtaccag attttagagg
    21841 ccccttcgcg cccggcgttt gatttcgcca tctgttccag gacggcaaaa ccttcgtcac
    21901 ggcgatcgct actaaacagc aatagcgcca gattgttttg caggcccgta ttgcccggcg
    21961 tatccgcatt aatgcgtttt agctgattaa tcgcttcgcc acggcgagcc ggaattttcg
    22021 ccaccgtact ccagtactcg acggcaatgt caccttccgg cggcgcaccg ttgaacagtt
    22081 tgttgtaact cgccacagct tcttctgcat gaccagtcgt cgcctgcaat cttgcctgtt
    22141 gcagtgcctg acgaccatcc ggcgtggaaa gcaacattgt ggtccgcgac gatttatacg
    22201 catttgaact cggcgctaac tgcgacagcc gatcgagctg tttttgcgcg ccatcaatat
    22261 cgccctgacg taacagagaa cggaaacggg cggcaacgac gtccgggtta ttcggatcaa
    22321 taagttccag ccgatacaac gactgttgca ccaggtcttc acgatgggtc gcttcgccta
    22381 accgaacttg ctccagcaac tgttgctgag cggttggtgc tgcctcgacc atcggcatga
    22441 cggccagacc gagggaaagc gtgaatatgt ttagtgtgaa tttgcgcatt cctggcccca
    22501 gtcaggtaat aactcacctt ttgtcgagaa gcggaaacgg tgttgatccc agccttgtcc
    22561 aaacagggtc agcacatagt tgtaataggc atcgctgccg ggaaagttat cggccacgcg
    22621 ctggcgctga acggcctgcg catcgcggtt ttgtaaaaag ggcagcatgg cggcagaaaa
    22681 accgaccggt cctttaccct gcgctttccc cgtagccaca tccacttttt ctggcggata
    22741 gccgtttttc tcggtgaatg tcgccatcgg tttaaaccgg ttgagcatcc gcgctttttg
    22801 cggatcgctg tcaggcatca tgcctaccca catgtaaacg cggatcgcgt cgtagctgct
    22861 gatcaatgtt ttttcggctt ttagctgcca gcctttgtct ttctcatagc gcacccagtc
    22921 tggcgaaaag cctttcgggg cggtttccag caataaacgt tgattggttt cgcgcagcgt
    22981 agtccacggc gcgccaaagc gggtgaaata ctgcgccagc gtcggcggca ggtagctggg
    23041 gttaaaacgc cagctgttat cctcagcaaa acccactttg cccggtaaca acatggaacc
    23101 cagcccaggc accgtcacca cttcctcccg cgcgatacgt tttaacaacg cgctgccgat
    23161 gtcggtataa cgctgctctt tccacaaacg ccccgcctcc agcaacgacc aggccatcca
    23221 gacatcacca tcggaggccg aattgctgtc cagcacttcc cacttactgt tctctttctt
    23281 gccccacagc caggcaggca aacgttcttt taaagaaccc tgagcgagat tgttctgcgt
    23341 ccagtcgaga atattatcga aagctgcacg gtcgttagcc gccagggcaa agaacatgcc
    23401 gtaactttgc ccttcggaag tggtgatttt gcgcgcgtcg ctggggtcga tgacgcgccc
    23461 ttcctgactg atgtaatcct ttttaaactg ttcccaggca ggccaggtac aggctgcctg
    23521 aacactaaag gcagccagca gcagcatcgt cacgagtcca ctacgcaaca cattcatctt
    23581 caattactcg ttatccgggt taagacgacg acggctgata atacgcagca aacgccacag
    23641 tacccaggcc agcagcacga cgctgattgc cgccagcacc gccagcagaa tcggatggtt
    23701 tgccagggca taccataagc gttcgaacca cggcagatgg ccaacgtaat agacgtcgcc
    23761 aacacgcagg ctattgatac cggactcgcg gatcaccgcg accgaaccga acatggtggc
    23821 gcgtttgccg ctatcgttca ccgcatcgtt aagcatttca taaccgcgtg ggctatctgc
    23881 cagcagcgca atcacgctac gctggtcgtt atacggcgac tggaagccaa tcaccgccgc
    23941 catcgcaccg gaagaggtca gcgttgactg ggtttctgcc gcgcgatcgc tctcgtccgg
    24001 cacaatgccg gggaacgggg tctggcgcat cggcgttttc acccagcttt cggtcgcctg
    24061 caccagcagg tcgatctgct tgtcgtcttt taatttgtcc gggataccac cgatgatcat
    24121 gatgtcggca tctttgccct gaatggtgct gccatcatcg gtcaccgtca gattaatcgc
    24181 cgggaagccc gtctgtgcgc cgataaaacc aacagtattc agcaaggttt ccatctgtgc
    24241 ttcgttaggc gttttcggca tcacggtgat ggtttgtgac agatccgcca tccggctgaa
    24301 cgggaagccc gcgttagcaa aggcgcgtag atccggcatc gggatgaagt ggtaatactt
    24361 cgagaagtcg atggtggaat catcaccaat caccacatga ttctgcaccg gctggaaggt
    24421 aatgcagtta tccaccgaac cgcccggcat cgggttcata tactcaaagt caaagcgcag
    24481 ctggttggtc gcgcccagtt tcagcgccgg aatagagaca tccgttttgc catccagcaa
    24541 accttgtaat accggaatcc gcagcagcag gcgattcgcc tcctgtttgc tgctcaggtt
    24601 gaaggattgc aggaactggt tattcaggct gatatccatc cgcgaactgt ctttcaccgg
    24661 cggcatggtg tagcgataat taatatccat atcaatgccg gtactgcgca tcaggtagag
    24721 atccggcggc aggtttagcg aaacgttaat cgctgctggc tcaagaccgc tggattgtaa
    24781 ctgttcttca taggttttca gttcaccaaa ggtaaccgga cgatcggtac gcacccagtt
    24841 cggcgcatcg tacggcttac gcggtagcag cggtttcact tcattcacta ccacgctttc
    24901 accacggaac agaatgttac cctgagcgat acctttcgct gcctgcaaca ggtctttgtc
    24961 gtcacgacca aacaccacca gcagtttgac ataaggattc tgcggatggt tgatcatctc
    25021 aatcaccggg gcttttaccg ccggatgatc gcgcaggaag tccgggcgtt tgtcattggt
    25081 ggcaaaaacg atggcattgc gatccggcaa ttggttatag agcaccggga agttctgccc
    25141 acgccagcca gaacgcgaac caaaccacga ggcgacaatg gcagatgctt gttgcagccc
    25201 aacatccggc gcacccgcaa agaccatcgg caaggtgtta gtacggttat cacgcgggtc
    25261 aaagaatggt accgggaagt gtgacaggtc attcttcaca ttcagggtct gataggtcaa
    25321 atccagtcca ctgctccgcc caacatccag ccaaagcgtg gtgctggccg ggttttcgca
    25381 cacgtcctga taatgaccga caaactccag ccgcacacgg ttgaagtcgg taataaacag
    25441 tgggttaatg ggcatttgtg ccagcgtttt tttacccaac tgttctttgg tcactggcag
    25501 cacgcccatc agttcatcat tgagataaac ctttaactgc gactggacag gcagtaacga
    25561 tggcgatggc gtgtattcga ggttgagcat cgccttcgtc accacttcat cgctgcgcat
    25621 accaaactca atgctgccgt tcgggttaat gccacgtagc accatgctgc ccggcggggg
    25681 cgcaatttgc gcaaaggtca gcttcacatc acgcgaagga ccgttctgcg ccacgatggg
    25741 ggcatcagcg ccctgcacgc ctggcatcac ttgccccacc tgcggatttt gttccgtcta
    25801 ggcggctaca gctggctcag cattgatcag tggttgcgtt gctggcgtcg cctgcgtcat
    25861 gaaagagggg aacgcactca tccccatagc cactgcacaa atccagaata gttttctttt
    25921 catcgcgtta tcatcattgt tgagccaaag cctgatccga tggttgtgcc gtttcgctcc
    25981 gctccgggcg gcgcggaata aacgatacaa cccaggaaac cagagaagtc agcacacgga
    26041 atatgccctt caccgaagaa ggcgcaaact ccgccagatg gcggtagcca cggaagccga
    26101 gcttcagaat atccagcaga ctttccagcg gcttatcttc cgggtagctg tcctgccaga
    26161 gcgcccatgt atccgcacgg gcaaacgtac actgcacaaa atcgatatgt tgctgggtgg
    26221 tgagcggcat taatttcaac ccaacttcat tacccatcac gcgcgccacc tgggtcggga
    26281 agacgtattc ctgctgaccg cgtttaagca acagattcac tttctgccct tccagaatct
    26341 gcgcctgacc gttgatcttg atccccaaac caccgtcgga gaaatcctga acggtacacg
    26401 agaagaggtg accatcttcg cgggcaattg ccgcgggcat cgtcatctcc acgcggtgcg
    26461 atcggcgtac ctgtttgctt tctaccgata ccgcaactgc gccgccaaga acaatcaggt
    26521 tgtagaatac ccacaccata ctgacgacca cggtgagcat ctcggttggc gggccataga
    26581 agtagcgcca gatgcctacc gcaacgccca ccaggttgag cagaacaagg aagatgtagg
    26641 gccgcgagat cacccagtcg acgtactctt cttccaccag tccacctttg gcggtgacgt
    26701 taaatttgcc tttgtgcggg ttaatcagcg ccaccagcgt cggtggtgcg atataccacg
    26761 ccagcaccgt ttcgtagatt tcactccaga aagagtggcg atatttgccc tggatcttgg
    26821 agttggtcag gctggcatgg atcatatgcg gcagcacgaa cagggcaatc atcaacgctg
    26881 gcgcatagat gatgtaggca tgcagcagca ggaacgccag cggcgcagtc aggaagatca
    26941 gtcgtggaat gcccgacaag aagtggaaca tggcgttgac gtaacatagc cgctgagcaa
    27001 acttcagccc tttaccggtg agcgggttat cgagacgaaa gatttgcacc atcccgcgcg
    27061 cccagcgaat acgctgaccg atatgcgccg acagactttc ggtcgccagc cccgccgcct
    27121 gcggaatacg catatatgca gaggtgtagc cacgacggtg caggcgcaaa gaggtatgcg
    27181 catcttcagt cacagtttcg acagcaatgc cgccaatttc atccagcggc ttacggcgaa
    27241 tcaccgcaca ggaaccgcag aagaaagtgg cgtcccacat atcgttgcca tcctgcacca
    27301 gaccatagaa cagcgtgcct tcgttaggtg ttttacggaa acgccccagg ttgcgttcaa
    27361 acgggtccgg tgagaagaag tggtgcggcg tctgcatcat cgccagttgt ttttctttca
    27421 ggaaccagcc catggtcatt tgcaggaacg atcgcgttgg tacgtggtcg cagtcgaaaa
    27481 tcgacacgaa ctcgcctttg gcatatttca gcgcattgtt gatgttgccc gctttcgcat
    27541 gttcatgagt ggtacgggcg atatatttca cccccacgtt ttgcgcaaac tggcgaaact
    27601 cttccctgcc gccgtcatca aggatccaga tgttcagctt gtctttcggc cagacgatac
    27661 ccagcgaggc gtaaatggta tttttcacca cgttgagatc ttcgttgtaa gtcgggacaa
    27721 agatatccac cgacggccac aacgacatat ctttcggcaa tggcaccggc tgacgattca
    27781 gcggccatac tacctggaag tagccgagca ccagcacaat ccacgcgtac gtttcggcga
    27841 acagcagaat aagcccgcac accaggctga ccggatcgtc ccagttcagc gtagaggtgt
    27901 agcgccacca gatataacgg caagaaacgg tcagcgacag cacaatcaac attagcgccg
    27961 agaagcgccc cggcatccgc cgtacgatca gcgctacccc ccacagcagc atcaggaaga
    28021 taaactgcgc cagcgggtta aacggctgag taacgcagat taacgccaga atcagcgaga
    28081 aggtgacgat gataccgagg atcaaccgcc gcgccccggc actcaaatgg ccgagctctt
    28141 ttttctcatc aagatgctgt gttttatggc taacgcgctc aggcagctcg ttcatccatt
    28201 gatggtaacg tccacgaata ttctgcagac ctgaaaatgc ccgcctgcgc ggtttcggcg
    28261 tttctttgcg cgatgcaccg atcagtaacc agcatgtttg aatgagataa cggaccgggt
    28321 ccagcggacg cggacgcgag gcgttgatat gcggatacag gtttttatgt tctgcgcgaa
    28381 tacgctgcca gcgcgggtgc tccagcggaa taaaaatcca ggccaggatc aaccaaaaac
    28441 agccgagcgt cgcgctgaaa gccgacgcgc cgtgacgacg ataatcgcga taacgcccga
    28501 taagccgcgc gttgaccggc gggataagca accaccgggt caggatactc atgatttact
    28561 cccgactggc gttttcagcc cggaatagtt caacaggcac cagttagcca gcgtcagtat
    28621 ctcttcagcc gccagcgcat cactgcgata ttcaccgact ggttgcttag ccgccaggca
    28681 ttcagccatc gcttcatcac gatgaatgag catcggcagt aatcggcgct ggctttgcaa
    28741 ccaaagctgg taaatatcgt cctgaacttg actgccgata cggaagtcat taatcaaaat
    28801 atgcgcgcca tccggcagcg cctgctgatg cagccggata tggcagttgg catccacatt
    28861 gacgattgcc agcgagtgat cgcacaaact cagcagctgg tgggttatct gcgaggaatc
    28921 acgcggtaag tcgattaaaa tccactggta acgcccgctg gcttttagtt gctgtaagcc
    28981 ggagcaaata tcgctcagcc gggtttgcca gtgctgtgga ttttcttgtt cttcaatgga
    29041 taactgacca aaaggcagca aatcgagctg cgaggtatag cgcaacccgg cgtcacgcca
    29101 gtcctggtca tccagcatag ctctggccca gccctgacgg tgggtaaaat caacgttaaa
    29161 tgacaggcgc aacaagttgt ccgggcaggc atcgaccacc aggacatttt ctcccaacat
    29221 ttgtaatgac caggctaatg cggcggtgat ggttgttgtc cccacgcctc cccgcacccc
    29281 ctgcaatccc agtacggcca ttcaccaatc ccctactttt gttgcgcaaa ctctgccagc
    29341 aacggccagc gttttaatgc cgcggccaac tgttcgcgtt gggaaatatc ggcataatca
    29401 atatcaggca gtgaaaatgc ctgctttaac gccaaaatat cattctggaa gatatagcct
    29461 atcgcgggat caggcagagt atctggttca ttgttattca tttgcttgat ccctatgaaa
    29521 taaccgtacc gttcatgaga caattctaac cgtgagcatt tcgccggttt attgtttacg
    29581 gcgagacggg aatttgctca ctttttgact ttccgttacg caacaaaaaa agttaaaaac
    29641 gaaaagacta aaatcacacg tcttgtgacc tgctaaattg attcacatgc taaatctgat
    29701 aagttttaat ttcaatggta ggtttatttc ttagctttcg ctagtaaact gataatcaga
    29761 caaaatgatg acatgaggga cattgtggac cctgtattct ctatcggtat ctcatcatta
    29821 tgggatgagc tgcgacatat gccagcaggc ggcgtctggt ggtttaacgt cgatcgccat
    29881 gaagatgcta tcagtctggc gaatcaaaca attgcatccc aggctgaaac cgcacacgtc
    29941 gcggtcatta gcatggacag cgatccggcg aaaatctttc aattagatga ttctcaagga
    30001 ccggaaaaaa taaaattatt ttcaatgcta aatcatgaaa aaggtctata ctatttggcc
    30061 cgtgatttgc agtgttctat tgatccccat aattaccttt ttattcttgt ttgcgcaaat
    30121 aacgcatggc aaaacattcc tgccgagcgg cttcgctcat ggttggataa aatgaataaa
    30181 tggagcaggt taaaccattg ttcgcttttg gtaattaatc ccggaaataa taacgataaa
    30241 caattttcat tgttgcttga ggaataccgt tcactttttg gtcttgccag tttgcgtttt
    30301 cagggcgacc aacatttgct ggatattgcc ttctggtgca acgaaaaagg ggttagcgcc
    30361 cgtcagcagc ttagcgttca gcaacaaaat ggtatctgga cattagttca aagcgaagag
    30421 gcggagatcc aaccacgcag cgacgaaaaa cgcattctga gtaatgttgc tgtactggaa
    30481 ggtgcgccgc cgctatcaga acactggcaa ctgttcaaca ataacgaagt cctgttcaat
    30541 gaagcccgta ccgctcaggc ggcgacggtg gtcttttctt tacaacaaaa tgcgcaaatc
    30601 gagccactgg cccgcagcat tcatactctg cgtcgccagc gcggtagtgc gatgaaaatc
    30661 ctcgtacggg aaaataccgc tagcctgcgc gccaccgatg aacgtttgtt attggcctgc
    30721 ggtgcaaata tggttatccc atggaatgcc ccactctccc gctgtctgac gatgatcgaa
    30781 agcgtgcaag ggcagaagtt tagtcgctat gtgccggaag gtatcactac cttgctgtca
    30841 atgacccagc cgctcaaact gcgtggtttc cagaagtggg atgtgttctg taatgccgtc
    30901 aacaacatga tgaataaccc tctattacct gcccacggta aaggcgttct ggttgcccta
    30961 cgtccggtac cgggtatccg cgttgagcaa gccctgacgc tgtgtcgccc taaccgcacc
    31021 ggcgatatca tgactattgg cggtaatcgg ctggtgctgt ttctctcatt ctgtcggatt
    31081 aacgatctgg ataccgcgtt gaatcatatt ttcccattgc cgactggcga cattttctca
    31141 aaccgtatgg tctggtttga agatgatcaa atcagtgccg agctggtgca gatgcgcctg
    31201 cttgccccag aacaatgggg catgccgctg cctttaacgc aaaattctaa accggtcatt
    31261 aatgccgagc acgatggtcg ccactggcga cgaataccag aaccaatgcg actgttagat
    31321 gatgctgtgg agcgctcatc atgatgacca tcagcgatat cattgaaatt attgtcgttt
    31381 gcgcactgat atttttcccg ctgggctatc tggcgcggca ctctttgcga cgcattcgcg
    31441 acaccttacg tttgttcttt gctaaacctc gttatgttaa accggccggg acgttacgcc
    31501 gcacggaaaa agccagggca accaaaaaat gactcaattt acgcaaaata ccgccatgcc
    31561 ttcttccctc tggcaatact ggcgcggcct ttccggctgg aacttctatt ttctggttaa
    31621 gttcggcctg ttgtgggcgg gatatcttaa cttccatccg ctcctcaatc tggtgtttgc
    31681 cgcgtttctg ctgatgccca ttccgcgcta cagcctgcat cgcttacgcc actggattgc
    31741 cctgccgatc ggctttgctt tgttctggca tgacacctgg ttgcctggcc cggaaagcat
    31801 aatgagccag ggttcgcagg tggcggggtt cagtaccgat tatttaatcg accttgtcac
    31861 acgctttatt aactggcaga tgattggggc catttttgtt ttattagtgg cctggttatt
    31921 cctgtcacaa tggattcgca ttaccgtttt tgtggttgcc attctgctat ggctgaacgt
    31981 acttaccctg gcgggaccaa gtttctcctt gtggccagcc ggtcaaccga cgaccactgt
    32041 aacaacgacg ggtggcaacg cagcggcaac cgttgcggcg acgggtggcg caccggtagt
    32101 gggtgatatg cccgcacaaa ctgcaccgcc aacaacggcg aaccttaacg cctggctgaa
    32161 taatttctat aacgcggagg cgaaacgtaa atcgaccttc ccgtcttcgc tgcccgctga
    32221 tgctcaacca tttgaactac tggtgattaa tatctgttcg ctttcctggt cggatattga
    32281 agccgccggg ttgatgtcgc atccgctgtg gtcgcatttc gatattgagt tcaagaactt
    32341 taactccgcc acctcctaca gtggcccggc ggcgatccgt ttactgcgcg ccagctgcgg
    32401 gcagacttcg cacactaatc tgtatcaacc ggcaaataac gactgctatc tgtttgataa
    32461 cctgtcgaaa ctgggcttta cccagcacct gatgatggga cataacggcc agttcggcgg
    32521 ttttttgaaa gaagttcgcg aaaatggcgg catgcagacc gaattgatgg atcaaacaaa
    32581 tctgccggtt attttgctgg gctttgatgg ttcgccggtt tatgacgata ccgccgtgct
    32641 taaccgctgg ctggacgtta ccgaaaaaga taaaaacagc cgtagtgcca cgttctacaa
    32701 cacgcttcca ctgcatgacg gcaaccatta tccgggcgtc agcaaaacag cggattacaa
    32761 agcgcgggcg cagaaattct ttgatgaact ggacgccttc tttactgaac tggagaaatc
    32821 gggtcgtaaa gtgatggtgg tcgtggtgcc ggaacacggc ggcgcgctga agggcgacag
    32881 aatgcaggta tctggcctac gtgatatccc tagcccgtct atcacagacg tccccgttgg
    32941 ggtgaaattc ttcggcatga aggcaccaca tcagggggca ccgattgtca tcgaccaacc
    33001 gagcagcttc ctggctatct ccgatctggt ggttcgcgtt cttgatggca agattttcac
    33061 cgaagacaat gttgactgga aaaaactcac cagtgggttg ccacaaacag caccggtctc
    33121 agagaactca aatgcagtag ttattcaata ccaggataaa ccgtacgttc gcctgaacgg
    33181 cggcgactgg gtgccttacc cgcagtaagc acgaaaaagg ccgcagagtt tcacccctgc
    33241 ggcctgatcc gggcgcaaat tgccattacg gcagcctgac gcccgcatga cacgttactt
    33301 ccgcttgttc agccagttca tgatcatact ggcaagaatg ccagcaatga ccggagccgc
    33361 taaatcatgc cagaaggcca tgcccagttc tgcgagcgtc atatagccgc ctgtgttgta
    33421 atgacaacgt ttcgcggcta ttcttgagtg gtctagagtc aagattagcc cccgtggtgt
    33481 tgtcaggtgc atacctgcaa cgtgcggggg ttttctctct ccagcaacca atgccaccag
    33541 ggataaagcc cccgcaacat tgcgcctcac cggaaccttt ccggcttgcc gctgattcta
    33601 cggccatttt tgttcgtcga aatccatcat ttcttcactc tgtgctgtgc attcagcaga
    33661 atattatccg cttgcaaccg ttgcattttt cttacgaaaa aggccgcaga gttttatccc
    33721 tgcggcctgg tccgggcgca aattgccatt acggcagcct gacgcccgtc tgacacgtta
    33781 cttccgcttg ttcagccagt tcacgatcag actggcaaga atgccagcaa tgaccggagc
    33841 cgctaaatca tgccagaagg ccatgcccag ctctgcgagc gtcatatagc cgcctgagtt
    33901 gtaatgacaa cgtttcgcgg ctattcttga atggtctaga tgcaagaatg gcccccgtgg
    33961 tgttgtcagg tgcatacctg caacgtgcgg gggttttctc tctccagcaa ccaatgccac
    34021 cagggataaa gccccccgca acattgcgcc tcaccggaac
//
LOCUS       DAOTRK010000039        33847 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.039, whole genome shotgun sequence.
ACCESSION   DAOTRK010000039 DAOTRK010000000
VERSION     DAOTRK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.039"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            108..737
                     /locus_tag="QIE41_001813"
     CDS             108..737
                     /locus_tag="QIE41_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDS3530707.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIITVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            764..2425
                     /locus_tag="QIE41_001814"
     CDS             764..2425
                     /locus_tag="QIE41_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDS3530708.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAKRALNDAINVLSDEQTSLK
                     FKLALLQSLPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGNAASGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            2664..2723
                     /locus_tag="QIE41_001815"
     CDS             2664..2723
                     /locus_tag="QIE41_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDS3530709.1"
                     /translation="MMKLIIILIVLLLISFHAY"
     gene            3040..3099
                     /locus_tag="QIE41_001816"
     CDS             3040..3099
                     /locus_tag="QIE41_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDS3530710.1"
                     /translation="MMKFVIILIVLLLVSFQAY"
     gene            complement(3220..4653)
                     /gene="hldE"
                     /locus_tag="QIE41_001817"
     CDS             complement(3220..4653)
                     /gene="hldE"
                     /locus_tag="QIE41_001817"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDS3530711.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4701..7541)
                     /gene="glnE"
                     /locus_tag="QIE41_001818"
     CDS             complement(4701..7541)
                     /gene="glnE"
                     /locus_tag="QIE41_001818"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDS3530712.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAISALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWSQLTGALTVHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAVVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(7564..8865)
                     /gene="ygiF"
                     /locus_tag="QIE41_001819"
     CDS             complement(7564..8865)
                     /gene="ygiF"
                     /locus_tag="QIE41_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDS3530713.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9107..9727
                     /locus_tag="QIE41_001820"
     CDS             9107..9727
                     /locus_tag="QIE41_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDS3530714.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9791..11029
                     /gene="cca"
                     /locus_tag="QIE41_001821"
     CDS             9791..11029
                     /gene="cca"
                     /locus_tag="QIE41_001821"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDS3530715.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGAEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11110..11931)
                     /gene="bacA"
                     /locus_tag="QIE41_001822"
     CDS             complement(11110..11931)
                     /gene="bacA"
                     /locus_tag="QIE41_001822"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDS3530716.1"
                     /translation="MSDMHSLMIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12022..12390)
                     /gene="folB"
                     /locus_tag="QIE41_001823"
     CDS             complement(12022..12390)
                     /gene="folB"
                     /locus_tag="QIE41_001823"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDS3530717.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12495..13112
                     /gene="plsY"
                     /locus_tag="QIE41_001824"
     CDS             12495..13112
                     /gene="plsY"
                     /locus_tag="QIE41_001824"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDS3530718.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRQHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13125..14057)
                     /gene="ttdR"
                     /locus_tag="QIE41_001825"
                     /gene_synonym="dan"
     CDS             complement(13125..14057)
                     /gene="ttdR"
                     /locus_tag="QIE41_001825"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDS3530719.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPRPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14264..15175
                     /gene="ttdA"
                     /locus_tag="QIE41_001826"
     CDS             14264..15175
                     /gene="ttdA"
                     /locus_tag="QIE41_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDS3530720.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIHDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELEVRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            15172..15777
                     /gene="ttdB"
                     /locus_tag="QIE41_001827"
     CDS             15172..15777
                     /gene="ttdB"
                     /locus_tag="QIE41_001827"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDS3530721.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15826..17289
                     /gene="ttdT"
                     /locus_tag="QIE41_001828"
     CDS             15826..17289
                     /gene="ttdT"
                     /locus_tag="QIE41_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDS3530722.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMSEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPMVLL"
     gene            complement(17332..18345)
                     /gene="tsaD"
                     /locus_tag="QIE41_001829"
     CDS             complement(17332..18345)
                     /gene="tsaD"
                     /locus_tag="QIE41_001829"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDS3530723.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     SAA"
     gene            18583..18798
                     /gene="rpsU"
                     /locus_tag="QIE41_001830"
     CDS             18583..18798
                     /gene="rpsU"
                     /locus_tag="QIE41_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDS3530724.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18909..20654
                     /gene="dnaG"
                     /locus_tag="QIE41_001831"
     CDS             18909..20654
                     /gene="dnaG"
                     /locus_tag="QIE41_001831"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDS3530725.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20849..22690
                     /gene="rpoD"
                     /locus_tag="QIE41_001832"
     CDS             20849..22690
                     /gene="rpoD"
                     /locus_tag="QIE41_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDS3530726.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22769..23275)
                     /gene="mug"
                     /locus_tag="QIE41_001833"
     CDS             complement(22769..23275)
                     /gene="mug"
                     /locus_tag="QIE41_001833"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDS3530727.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            23400..23475
                     /locus_tag="QIE41_001834"
     tRNA            23400..23475
                     /locus_tag="QIE41_001834"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23433..23435,aa:Ile,seq:cat)
     gene            complement(23529..24293)
                     /gene="nfeF"
                     /locus_tag="QIE41_001835"
     CDS             complement(23529..24293)
                     /gene="nfeF"
                     /locus_tag="QIE41_001835"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDS3530728.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRAERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVMPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDHACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            24570..25193
                     /gene="nfeR"
                     /locus_tag="QIE41_001836"
                     /gene_synonym="yqjI"
     CDS             24570..25193
                     /gene="nfeR"
                     /locus_tag="QIE41_001836"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDS3530729.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(25298..26818)
                     /gene="aer"
                     /locus_tag="QIE41_001837"
     CDS             complement(25298..26818)
                     /gene="aer"
                     /locus_tag="QIE41_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDS3530730.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            27236..28615
                     /gene="ygjG"
                     /locus_tag="QIE41_001838"
     CDS             27236..28615
                     /gene="ygjG"
                     /locus_tag="QIE41_001838"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDS3530731.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALSECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(28657..28989)
                     /locus_tag="QIE41_001839"
     CDS             complement(28657..28989)
                     /locus_tag="QIE41_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDS3530732.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMEKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            29208..30191
                     /gene="ebgR"
                     /locus_tag="QIE41_001840"
     CDS             29208..30191
                     /gene="ebgR"
                     /locus_tag="QIE41_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDS3530733.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPSSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30375..33467
                     /gene="ebgA"
                     /locus_tag="QIE41_001841"
     CDS             30375..33467
                     /gene="ebgA"
                     /locus_tag="QIE41_001841"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDS3530734.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISTMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERLVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVIPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPISGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            33464..>33847
                     /locus_tag="QIE41_001842"
     CDS             33464..>33847
                     /locus_tag="QIE41_001842"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDS3530735.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQVVICDIHEAYRFIC"
BASE COUNT         8227 a         9170 c         8602 g         7848 t
ORIGIN      
        1 tcttccagaa cttctctaac atgtgttatt attaacaaag ataaatgtaa gcaaggaaac
       61 tatcctaaag taaatattgc gtcactaaat ggacattgga gtgacatatg attttattcg
      121 ccgactataa caccccttat cttttcgcta tttcctttgt actgttgatc ggccttttag
      181 aaatttttgc gcttatctgc ggtcacatgc tctccggtgc acttgatgca catcttgacc
      241 attatgattc cataaccaca ggtcatatta gccaggcact tcattatctc aatatcggaa
      301 gattgccagc tctcgtcgtt ctctgcttgc tggctggttt ttttggtctt attggcatcc
      361 tgttgcagca tgcctgcatc atggtctggc aatcaccact gtcgaacctg ttcgtggttc
      421 ctgtcagttt actgtttacg attattacgg tgcattatac cggtaaaata gttgcgccct
      481 ggattccacg cgaccatagt tcagctatta cagaagaaga atatattggt agcatggcat
      541 taattaccgg tcatcaggcc acttcaggta atccctgcga aggaaaactc accgatcaat
      601 ttggccaaat acattattta ctactggagc ctgaagaagg aaaaattttc actaaaggag
      661 acaaagtgtt aattatttgc cgactttctg caacgcgata tttggcggaa aacaatccct
      721 ggccccaaat attgtaattt attaatcaaa aggaaattta ataatggatg atattgttaa
      781 ttctgtgccc tcctggatgt ttaccgcaat tattgccgta tgcattctgt ttattattgg
      841 aattattttc gccaggctct atcgtcgcgc ttcggcagag caagcttttg ttcgtactgg
      901 tttaggtggg caaaaagtgg taatgagcgg tggcgcaatc gtgatgccga tctttcatga
      961 aataatcccc atcaatatga atactctgaa gctggaagtc agccgctcaa ccattgatag
     1021 cctgattacg aaagatcgta tgcgcgtcga tgtcgttgtc gctttctttg tgcgggtaaa
     1081 accgtcagtg gaaggtattg ccaccgccgc ccagacgctg gggcaacgca ccctgtcgcc
     1141 tgaagactta cgtatgttgg ttgaagataa atttgtcgat gccctccgtg caacagctgc
     1201 acaaatgacc atgcatgagt tacaggatac ccgcgagaac ttcgtgcagg gcgtacaaaa
     1261 taccgtagct gaagatctgt cgaaaaatgg cctggaactg gaaagcgttt cacttaccaa
     1321 ctttaaccag acgtcgaaag aacatttcaa tcctaacaac gcctttgacg ccgaaggttt
     1381 aaccaagctg acgcaggaga cggagcgccg tcgtcgcgaa cgtaacgaag ttgaacagga
     1441 tgtagaagtt gcggtgcgtg agaaaaaccg tgatgcgctt tcgcggaagc tagagattga
     1501 acaacaagaa gcgtttatga cgcttgagca ggagcagcag gttaaaaccc gtaccgctga
     1561 gcagaatgcg aaaattgcgg cttttgaggc cgagcgtcgt cgggaagcag agcagacgcg
     1621 aattctggct gaacgacaga ttcaggaaac ggaaatcgac cgcgaacagg ccgtccgttc
     1681 cagaaaagtt gaagctgaac gcgaagttcg cattaaagag atcgaacagc agcaggtcac
     1741 cgaaatcgct aaccagacga aatcgatcgc tattgccgcc aaatcggaac agcagtcaca
     1801 agcggaagcg cgtgccaacc ttgcacttgc agaagcggta agcgcccagc aaaacgtaga
     1861 aacaactcgc cagaccgcag aagccgatcg tgctaaacaa gttgccctaa tcgctgccgc
     1921 gcaggatgca gaaaccaaag cggttgaact gaccgtgcgg gcgaaagcag agaaagaagc
     1981 cgcagagatg caggcggcgg caatcgttga gttagccgaa gctacgcgta aaaagggtct
     2041 ggcggaagca gaagcgaaac gtgcgctgaa cgacgctatc aacgtacttt ctgatgaaca
     2101 aaccagcctt aaattcaaac tggcgctatt acagtcgtta cctgcagtaa tagagaaatc
     2161 cgttgagccg atgaagtcta tcgacggcat caagattatt caggtcgatg gattaaaccg
     2221 aggtggtgct gcggggaatg cggcatcagg cagcgttagt ggcggaaacc tggcagaaca
     2281 ggcattatca gccgcactct cttaccgcac acaggcacca ctgattgact ccttgctcaa
     2341 tgaaattggc gtttcaggcg gctcactggc tgcattgact tcacccttaa cctcaacaac
     2401 tcccgtcgca gaaaacgtag aataaatttt caactatgaa catccctggc ccaaaaagcc
     2461 ggggattctt tatatctgcg ttccgataaa aagtgcaaat gatcgggctg ttgcagcatt
     2521 ttgcgtgacc gctcggggaa ggcaaaactg cctctgggta agcattgcgc ggggtccggc
     2581 gttcatcaac aatcgggaag tcagcaaggg gctgaaacgg gaaagcccct cccgaagaag
     2641 gggccttgta taaggaaagg gttatgatga agctcatcat catactgatt gtgttgttac
     2701 tgataagttt ccacgcttac taacagccaa tcagaggggg gagaaatcct ccctcaccct
     2761 tgttccttta ctttaggttg aaaaaaaatc agcgtcaaga aggtttcatt aacacatatt
     2821 cctggcccaa aaaaccgggg attttttata tctgcgttcc gcaaaaaact gcaaatgctc
     2881 aggtcgttgc agccttttgc gtgaccactc ggggaaggca aaactgcctc tgggaaagcg
     2941 ttgcgcgggg tctggcgctc atcaacaatc ggggagtcag caaggggctg aaacgggaaa
     3001 gcccctcccg aagaaggggc cttatagaag gaaagggtta tgatgaagtt cgtcatcata
     3061 ctgattgtgt tgttactagt aagtttccag gcttattaac aaccaattca gaggggggag
     3121 aaatcctccc ttacccttgt tcctttactc taggttgaaa aaacaacagc gtcaataggc
     3181 ctgccaggtt cgaggcgaga tctgtgaacc gctttccggt tagccttttt tatcctgttg
     3241 gatcttcttg atgatgttgg tcgtcgagca accgtcttca aagttgagca ccagcacttc
     3301 gccgccgttg gcccagactt ctttactccc ggcaatctct tctggtttat agtcgccacc
     3361 tttcaccagc aggtctggca ggatcccggc aattaagcgc tgcggcgtgt cttcttcaaa
     3421 cgacaccacc cagtcgaccg cttccagtgc gcccagcaca atcatacgct gttcgagcgg
     3481 gttaacgggg cgggaatccc ctttcagccg tttggtggag gcatcgctgt tcacagcgac
     3541 aatcaggcgg tcacccagtt tgcgggcatt tgccagatag gagacgtgcc cggcgtgcag
     3601 gatgtcgaag acgccgttgg tcatcaccac tttttcaccg cgtttacgcg ccgccgctac
     3661 agcaagcttc agttcctctt cggtcatcac accaaagccg gtatctgcac gtccacgtac
     3721 tgcattttcc agctcgatcg gcgaaaccgt ggatgtcccc agtttgccga ccaccacgcc
     3781 tgccgccgca ttggcaaaga agcaggcttc ttccagcgaa ttacccgcag ccagcgttgc
     3841 cgccaggacg ccaatcaccg tgtcgcccgc accggtaacg tcatatactt cctgcgcctg
     3901 agttggcata tgcagcggtg ctttacccgg ttgcagcagc gacataccct gttcggaacg
     3961 ggtcactaac agagccgaga gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat
     4021 ctcttcttcg gtcttacatt taccgacaac agcttcaaat tccgagagat tcggcgttaa
     4081 cagcgtagcg ccgcggtagc gctcaaaatc ggtacctttt ggatcaatca gcaccggaac
     4141 acccgcttta cgcgccagtt ggatcatctg ctgtacgctt gccagcgcac ctttggcgta
     4201 gtcagaaagc accagcgcgc caatcgaact cagtgcctga ttaatccgct cgtgcagcgg
     4261 ctgcggatca accccttcga aaccttcttc gaaatccaga cggatcagct gttggttgcg
     4321 ggaaagtacc cgtaatttgg tgatagtcgg atgcgtcggt acagaaacaa agtcgcattt
     4381 gacgttgacg tcggccagag atttactcag cgcgcgcgcc gcatcgtcaa tgcccgtcaa
     4441 cccgaccagg cgtgcattag caccgagaga agcgatattc atcgccacgt tagccgcgcc
     4501 gcccggacgt tcttcgatgg tatttacttt aaccacgggc accggcgctt ccggcgagat
     4561 acggctggtg ggaccgtacc agtaacgatc cagcatcaca tcaccaacca ccatcactcc
     4621 tgcacgttca aactctggca gcgttacttt cattcctgtc tcctgagaga ttcaaaattt
     4681 gcgcgcgata ataccatact tcattcttcc accagccact tctgccagct tgcccgcacc
     4741 agttcacgct ctgcggtgaa gcaatcctcc gacacatggc ccggcaattc ctgtaatgcc
     4801 agatgatgaa gttcatcgcg cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc
     4861 tcttccataa tgtcgttttg cgccagtagt tccagaatgc gcacgttgtc tgaccagcgc
     4921 gttaacttcg gtttttcatg agcgtagcgc aacaccagat attgagtaat aaattcgata
     4981 tcggtaattc cgccctcatc ggctttgata tcaaagcgat cgcgatgttt attgccgaga
     5041 tgagcacgca ttttctcgcg catttcccgc acttctgttt gcagggtttt gccttcgcgc
     5101 ggcagcgtca taatctcccg gcgtactgcg tcaaagtgcg cggtgagctg cggatcgccg
     5161 tacactacac gcgcacgcac cagcgcctga tgttcccacg tccaggcctc gtttttctga
     5221 taatcggcaa acgcttctgc ggatgtcacc agcattcccg ccgccccgga cggacgcaac
     5281 cgtgcatcca cttcatacaa aatgccggaa gaggttcgcg tactgaacag atgcataatg
     5341 cgttgcgcca gacgcagata aaactgtcgc ccgtcgattt cccgctcgcc gtcagtcatc
     5401 gcatccattg ggcaatcatg gaggaagatc aggtcaagat cggaactgta gcctaactcc
     5461 cagccgccca gcttgccgta gccgaccacc gcaaaaccac gcccttcgcg ttcgttcagg
     5521 tgatttggct taccgtagcg ggcaaccatt tgaacccacg cctgctgaac aacggcgtca
     5581 atcatggctt ccgccaacca ggttaagtga tcgctcactt tcattaccgg cagcgtaccg
     5641 gcgatatccg ctgccgcaat gcgtaacagt tgcgcctgtt tgaactgacg cagcgcctca
     5701 agctgttgtt cttcgtcatc ttccggcacg cgcagcaaat actggcgcaa ctcatcacgg
     5761 taagcatcgg tcgccgtcgg ctggtagaga gtgtttggat cgagcagttc atccagcaat
     5821 aacggatagc gcgccagctg gctggcaatc atcggcgatg cggcacacag agaaatcaaa
     5881 tgcttaagcg ccgcggggaa ttcactgagc aattctaaat aggtggtgcg ggtaacaatc
     5941 cccaccagca aggcggtaat gcgcgacagc gtgacaacag cgtcttcacg cgcacagaca
     6001 tcacttagca gatgcggcat cagatggtcg agcacctgac gcccgcgcgg cccgatggta
     6061 cgcttatcca gctctttgcg aaaatcggca atcagcgtca gcacctgttt gcgatcgtcc
     6121 tcgctaagat gcgccagcac tggtgtagtg tcatcttcct gcaacgcgtc ctgccacagc
     6181 tcacgccact gttccgacag cgactcttcc tgagtttcgc tttcgtcgtc gccaatcaat
     6241 tcattaaaca cccggcgcac attggtcata tgtacggtca gcgccccggt caattgcgac
     6301 cagtcagcaa agtccatcgc ccacgccagc cgcgcacggt taagctcatc agaaggaaga
     6361 gtctgggttt gttcgtcgtt aatgctttgc agcaggtttt ccagacgccg caggaacaga
     6421 tacgccactc gcaattgttc agcatcgttt tcagaaagca gatgcagcgc ggaaatggcg
     6481 ctgagcgttg gcagtaaaga gcgcgattgc agcgacggtt cacgcccgcc gcgaatgagc
     6541 tggaacacct ggacgataaa ttcaatttcg cgaatgccgc ctgcgccaag tttgatgttg
     6601 tcggtcaaac cgcgccgacg cacttcacgg gcaatcatcc ctttcatgtt gcgcagcgac
     6661 tgaatcacgc tgaaatcgat gtaacgacgg aaaacaaacg ggcgcagcat cgcacgcaac
     6721 tcgttagcat agacgccttc gctatcgccc ataatccgcg ccttgaccat cgcgtaacgc
     6781 tcccagtcgc gcccctgctc ctggtaataa tcttccagtg ccgcaaagct cagcaccagc
     6841 gggccacttt cgccaaacgg acgcagtcgc atatccacgc gatagacgaa gccatcctgc
     6901 gttggttgat ccagcacttt aatcagccgc tgccccatgc gggtaaaaaa ctgggcgtta
     6961 tccagttccc ggcgtccacc ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg
     7021 atatctgatg agaaattcag ttccccaccg cccagcttac ccatgcctaa aatcagcagc
     7081 ggttgcgctt cgccctgcgc attgcacggc gttccccatt cgcggcagca ggcgtcatac
     7141 agccagtcac gcgccgcaac aatcagcgtc tccgccagat ggctgagctg ctgcaaaatg
     7201 ctctcttcag taaccaatgc cagcgtttgc gcccaggcga tgcgcaccat aatgcgccgc
     7261 cggaatagcc gcagctcgcg cattaacccg gcttcgtcac tcacattact gagcgcctcc
     7321 tgcaaccatg ctgcgtaatg ctgccattcg tcggcctgcg gcggctggct ttccagttcc
     7381 gtcagccact caggatgcgc aatcacgctg tcctgcacaa aatcactaaa agtaagtact
     7441 gacttcgcct gtgcgctaag tgattcctcg gctaaaggct ctggcagccg ctcaacaacg
     7501 gtctgccagt actgctgtaa cggtgaagag agcggcttca taagcgattt tatccttgcc
     7561 tgattaacgt tttccgctgt gcaaccagaa cggttcctga ttgtttgcct cattacggaa
     7621 atgttcaatt tcgatgcgtt gcccggtcgc aatagcgtga cgcagcccct gccagttctc
     7681 cagccaggct tgcgcgacga caggatcgta gtaacccgcc agcaacagga ttgagtcaat
     7741 atcacgcgtc aggcgtggca actggtcacg gtagcgatcg cccaacggct ggcagaaaac
     7801 gcttttcagt tcagcggcat ggcgggaaag atggatatcg gcaaagcgtt tgaaggaatc
     7861 gctcatttta ctctgcgctt tggcatctaa aaatggctgc catgctttgc ttaccaacca
     7921 ttcggtcaac gccagcttcg ccattgccgt ttcggtagag tagaccgccg tcacggcaga
     7981 aaccgcagaa gcaatggtcg cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc
     8041 tttacgcggc acaataccac cgaacagcat cagcgtatga cggaccaggc taatggctgc
     8101 cagcacctgt tctttcgccg catcgttgcc gcgtacccac agttcttcat gatactgcca
     8161 ttgcgctaac gccagctcga acgccgcttc cagcccctgt tccacatcgg cttttgccgc
     8221 aacatgcaaa atggtggtcg gtttgatttc acgcgccgga ttgccctgcg ccagatgata
     8281 gccacgcgcc gctttgctca ggctgccctg gcgtaatccg gtttgcgata ccagttggtt
     8341 cgccagtttc agcaccgcgc gcgtgtcgcc gctaagcagt tccagttcca gctcgcagat
     8401 aggttcagca aattcacccg ctttcacttc cccctggtcg agggcgattt caatttggct
     8461 accatcaacc tctaccagcc acttttcgcg ataaaaatcg gtgctgaaca gcggttgtac
     8521 gcgggaagcg agatcggcag gcaattcgcc gttcggccag acttccgtcg gtaactgcgc
     8581 caggtcgagc gtcggttcgc tcaacgccac gttatattcc gggcgctggt gtaagccgcc
     8641 tgtcactctt cctgcaactt tcatggtcat ctcatagcga ccgttttcgc cacgaatacg
     8701 caagcccatg tcgtgcccac gcagccagtt atccggcgtt tcgtagtaaa tattcaacaa
     8761 ctgcacgggg tcatggtgct cgccgcccag cgtattgaga tggtcacgca acgcctcaac
     8821 ggcactgtga ttaacaataa actttaattc gatttcctga gccatggcct ttacttatcg
     8881 gttatgtcac ataagtgacg atgaacggcg aacttaatgc gatctttttt gtcagtagat
     8941 agtattttgc gccaaattgc catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca
     9001 gtggcaagac ctgacacaga ggattccgat tgatgtcgaa tgctttgcgt agcgacactg
     9061 atgccactac tatcgttcca ctttccatga caataacgac agcctgatgc caaaattacg
     9121 cctgatcgga ttaactttac tcgcacttag cgccactgcc gtctcacacg ctgaagaaac
     9181 gcgctatgtt tccgacgaac tgaatacctg ggtccgtagc ggtccgggag atcattatcg
     9241 cctcgtgggc acggttaatg ccggcgagga agtgacctta ttacaaactg acgccaacac
     9301 caattatgcc caggtgaaag acagctctgg ccgtaccgcc tggatcccgt tgaaacaact
     9361 tagcactgag ccaagcctgc gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct
     9421 gaccgataaa ctcaccaata tcgataacac ctggaatcag cgcaccgcag aaatgcagca
     9481 aaaagtggcg cagagcgaca gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa
     9541 aaacgagctg attgtcgcgc agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa
     9601 acagcgcacc atcatcatgc aatggtttat gtatggcggc ggcgtgctgg ggcttggctt
     9661 gctgctcggt ctggtactgc cgcacctgat cccaagccgc aaacgcaaag atcgctggat
     9721 gaactaaatc gccttcttcg ttgcactgtc ttattattag gaattgtgta tttgacgaag
     9781 ggatgatggc gtgaagattt atctggtcgg tggtgctgtt cgggatgcat tgttagggct
     9841 accggtcaaa gacagagatt gggtggtggt cggcagtacg ccacaggaga tgctcgacgc
     9901 gggctaccag caggtaggcc gcgattttcc tgtttttctg catccgcaaa cgcatgaaga
     9961 gtatgcgctg gcacgtactg aacggaaatc cggttccggt tacaccggtt ttacctgcta
    10021 tgccgcaccg gatgtcacgc tggaagatga tcttaagcgt cgcgatctga ccattaatgc
    10081 gctggcccag gacgataacg gcgagattat cgacccgtac aacggtctgg gcgatctgca
    10141 aaatcgtctg ttgcgccatg tttcccccgc ttttggcgaa gatccgttac gcgtattgcg
    10201 cgtggcgcgt tttgctgcgc gttatgccca cctcggtttt cgtattgccg atgaaactct
    10261 ggcactgatg cgcgagatga cccatgcggg tgaactggaa cacctgacgc ctgaacgggt
    10321 atggaaagag acggaaagcg ccctcaccac ccgcaatccc caggtgttct tccaggtact
    10381 gcgcgattgt ggtgcactac gcgttttatt cccggaaatt gacgccctgt ttggcgttcc
    10441 ggcccctgcc aggtggcatc cggaaatcga caccggtatt cacaccttaa tgacgctctc
    10501 aatggcggcg atgctaagtc cgcaggtcga tgtccgtttc gcaactttat gccacgatct
    10561 cggtaaagga ctgacgccac cagaactttg gccgcgtcat catggtcatg gcccggcggg
    10621 tgttaagtta gtggaacaac tctgtcagcg tctgcgcgtg ccaaatgaaa ttcgcgattt
    10681 agccagactg gtggctgagt ttcacgatct catccacacc ttcccaatgc tgaacccaaa
    10741 aaccatcgtc aaattgtttg attccatcga cgcctggcgt aaaccgcagc gtgtcgagca
    10801 actggcgctg accagcgagg ctgatgtgcg cggcagaacc ggttttgaat cagcggacta
    10861 cccgcaaggc cgctggttgc gcgaagcctg ggaagtggca cagtcagtgc cgacaaaagc
    10921 cgtcgttgaa gcgggattta aaggtgcgga gattcgcgag gagctgaccc gacggcggat
    10981 tgcggcggta gccagctgga aggaacaacg ttgtccaaag cctgattaag cacttgaatt
    11041 tcaatttatt gcaaacaccg gccaggtcgg ataagacgca caagcgtcat atccgacact
    11101 attcctcaat caaaagaaca cgacatacac cgcagccgcc acaataaagc gataaatggc
    11161 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11221 caccacaaaa gcggtgataa acccaacggc aaacatcggg atatcgccgg ttgtcaggaa
    11281 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggca ccgccagcag
    11341 gaacgaaaac tcggaagcag cgtaacggct cacccccatc agcatcccac ctgaaatggt
    11401 cgccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11461 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    11521 ttcagcggca atcagcaaca gaccgccaac gaccagtgca tacatcacat ttatcgggtt
    11581 aaacaatgat ttaatcgtgt cgtggaacaa tagccccagt actaccgctg ggatcatgcc
    11641 aagcagaatg tggatcagcg ttaaacggcc tttgctttct ccttcgtgct gcaacgggcg
    11701 accaaagtga ataccaataa ggccaaacag acgtcgccag aacatgacaa ccactgccag
    11761 aattgatcct aactggatca cgacttcaaa ggttttcgcc gtgtcgccct caaaccccaa
    11821 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    11881 ttcgaccaca cccaatattg ccgctatcat cagcgagtgc atatcgctca tcaataaacc
    11941 cctaaattat aaaaatgtaa cgcttgtccg aactactgca gtatgaccag gttataaccg
    12001 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12061 gacgccaaca ttcgccgccc gcgccactgc gcctggcttg ctgagtttga tacgcaccca
    12121 cggcgagttg aagcgtgcta acagcagctc cgccacctct tccgccacac gttccaccag
    12181 cgcaaaacgc gccccctcga cgtggctgac taccgtttct gcaatgtcag cgtaactgag
    12241 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccacgcca tttcgatatc
    12301 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatgg tggtgattac
    12361 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12421 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12481 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    12541 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    12601 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    12661 tagcagtgct gatctttgac gttctgaaag gaatgttacc tgtctggggg gcatatgaat
    12721 taggtgtcag ccccttttgg ctaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    12781 ggcccgtttt cttcggtttt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    12841 caccaattgg ctgggatctt accggagtaa tggcgggaac ctggttactg accgtgctat
    12901 tgagcggata ttcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    12961 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13021 gtcagcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13081 tcaaaagaaa gcgcgaaaag gatcctgagt gatttttcgc gagtctacat aacctgatag
    13141 ccttcgtcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13201 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13261 gcatactccg gcaatactcg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13321 tcggaacgca acataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13381 ttggaggaaa gatgtccgct taccttcacc gattttttct cctgaccgtt ccccaactcc
    13441 catattccgt gggtcatatc gcgctctttg gtcaccaggc agtcatgacg acttaattcc
    13501 tgtaaggatt gtggcctcgg atatttttgc agatactcag gtgctgcgca taatattctt
    13561 ttattttttg tcaaaagatg cgcaatataa taatcaggaa ttgcgtcatt aatacgaata
    13621 tccagatcaa tattatcctg cactaaatca atttgtcgat cgaacaattc aaaatgcacc
    13681 tgtaactcag gataattgcg catcagttcg gtaatagctg gcgcaatatg gctgcgccca
    13741 aaaccaaaac tgcaaccaat gcggatcatc ccctccgggc gcgttttgat ttgcgtgacg
    13801 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgctgg
    13861 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagagttgt tgccagcgta
    13921 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tttgcccaag cgtcgccgct
    13981 gcagcactaa aactcccgct atggacgatt tccaccagta cctgcaagtc tttggctaaa
    14041 ggccagctat ttagcattgt taaatatccg cagttttgat aaacgcagtg tatttcgcaa
    14101 aacattacca ttccctgctc tggcgcaata ttcgttctta ttttcgcgtt gattcacgaa
    14161 ctgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14221 tggcggtgaa tttttccaga caaatacata aactggagtt gccatgatga gcgaaagtaa
    14281 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14341 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14401 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14461 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    14521 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    14581 caaagcgcca ctgcgtcaca atgcggtaga aatttttgac gaagtaaata ccggcaaaaa
    14641 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cacgacaatg acgatgcgga
    14701 aatcgaagtt tacatggcag gcggcggctg cacgctacca ggccgctcga aagtgttaat
    14761 gccgtcagaa ggctacgaag gcgtagtgaa attcgtcttc gaaaatatct ccaccctcgc
    14821 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    14881 cgccgtgctc tcgcgaaaag ccattttgcg cccgattggc agccgccatc caaatccaaa
    14941 agcggcagag ctggaagtac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15001 agggctgact ggcaacagtt cagtgatggg cgtgcatatc gaatctgccg cccgccatcc
    15061 gtcaaccatc ggcgttgctg tttctacagg ctgctgggcg catcgtcgcg gcacactgct
    15121 ggttcatgcc gatctctcct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15181 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg cgatgtgatc
    15241 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15301 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15361 cgcaaaaacg gcgacaaatg ggagatggtt tccgtcggtc cgacaaccag tatgcgtatg
    15421 gaaagttttg aacgtgaatt tatcgaacag accggcgtga aactggtggt cggcaaaggc
    15481 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctgca tgtgattttc
    15541 ccggcaggct gcgcagtact ggcggcaacc caggtggaag agattgaaga ggtgcactgg
    15601 acagaactcg ggatgccgga gtcgctgtgg gtctgccggg tcaaagagtt cggtccgctg
    15661 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    15721 gaacgccgcg atcccatcgt ggaagagatt tgcgagcacg tccactacat caaataaccc
    15781 tcccggagag gctcaccccc tctccttttt cgcaggcata acacgatgaa accttccact
    15841 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    15901 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    15961 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat ctccattatc
    16021 gccattctct cgccgtggct gctgtttagc ccggagcagc tcgcccagcc aggttttaag
    16081 ttcaccgcca aatccctctc ctgggcggtt tctggttttt ctaattcggt tatctggctg
    16141 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggctggg gcgacgtatc
    16201 gcgctgattc tggtgaaaaa gatggggcat cgtacgctgt ttctcggcta tgcggtgatg
    16261 ttctccgagc tgatcctggc acctgtaaca ccgtccaact cggcgcgcgg tgcggggatt
    16321 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16381 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16441 gccattttcc tgacggcgat ggcacctaac ttgctgttaa ttggcctgat gaaaagcgca
    16501 tctcacgcca cactgagttg gggcgactgg ttcctcggga tgttgccgct cagtatttta
    16561 ctggttctgc tggttccctg gctggcttac gtgctgtacc cgccggtact gaagtctggt
    16621 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttctcgt
    16681 gaaaaacgga tgctggggct gatggtaggc gcactggtgc tgtggatttt cggcggtgat
    16741 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    16801 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    16861 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    16921 ctgttagcag gcagcttaag tggttattcg ccgacgatgg tgatggtggc gttgattgtg
    16981 gtgttttatc tactgcgcta cttctttgcc agcgccacgg cgtatacctc ggccctcgcg
    17041 ccgatgatga ttgccgccgc gctggcgatg tccgaaatcc cgctgccggt gttctgcctg
    17101 atggttggcg cggcaattgg tctggggagc attcttacgc catacgccac cggacccagt
    17161 ccgatttact acggtagtgg ttatctgcca acggtggatt actggcgact gggggcaatt
    17221 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccatggtg
    17281 ttgctttaag tgaggacggt gccggaagtg actctccggc acccaatagt tttacgcagc
    17341 cgataactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17401 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaattc
    17461 cggacgcgcg tagaacactt cgccacggcg ttttttcatc atttcagcca gcttcgcccg
    17521 taacgtgcgg ttagcactca cgccgcctgc cattaccagt cgcttaaagc ccgtctgatc
    17581 cagcgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    17641 gatatcagca cgcgtctggt cgtcggtgcc gttatcacga atggtatttg ccgcgaaggt
    17701 tttcagacca gaaaagctga aatccagccc tggacggtcg gtcatcgggc gcgggaagac
    17761 aaagcgcccg gctgtaccct gagccgccat tttcgacaat aacggcccgc caggataatc
    17821 cagccccagc agcttcgcgg ttttatcaaa cgcttcacca gccgcatcat cgatagactc
    17881 tccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    17941 aaccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18001 atgtccttcc atatggtgta ccgggatcgc aggaacgtcc caggcaaacg ccagagaacg
    18061 ccccacggtc gcgccaacca gcagcgcgcc gactaatcca gggcctgcgg tataggccac
    18121 agcatcaatg tcttttgccg ttaaaccgga ctctttcagc gccgcctgaa tcaacggtac
    18181 ggttttacgc acgtgatcgc gggaggccag ttcaggcacg acgccgccgt agtcagcgtg
    18241 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18301 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18361 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18421 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa aggccgcggt
    18481 gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc attttgaaat aagctggcgt
    18541 tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc acatgccggt aattaaagta
    18601 cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca agcgttcctg cgaaaaagca
    18661 ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa aaccgactac cgaacgtaag
    18721 cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac tggctcgcga aaacgcacgc
    18781 cgcactcgtc tgtactaatt ccccgagagc gttgctctcc gatcagaccg agttgtagtt
    18841 gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc gtttatgaat tgctaaaaat
    18901 cggggcctat ggctggacga atcccacgcg tattcattaa tgatctgctg gcacgcactg
    18961 acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa gcagggcaag aatttccacg
    19021 cgtgttgtcc attccacaac gagaaaaccc catcattcac cgttaacggt gagaaacagt
    19081 tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat cgacttcttg atgaactacg
    19141 acaagcttga gttcgtcgaa acggtcgaag agctggcagc aatgcacaat cttgaagtgc
    19201 catttgaagc aggtagcggc cccagccaga tcgagcgcca tcaacgacaa acgctttatc
    19261 agttgatgga cggtctgaat acgttttacc aacaatcttt acagcaacct gttgccacgt
    19321 ctgcgcgcca gtatctggaa aaacgcggat taagccacga ggttatcgcc cgctttgcga
    19381 ttggttttgc gccccccggc tgggacaacg tcctgaagcg gtttggcggc aatccagaaa
    19441 atcgccagtc attgattgat gcggggatgt tggtcactaa cgatcagggt cgcagttacg
    19501 atcgtttccg cgagcgggta atgttcccca ttcgtgataa acgcggtcgg gtgattggtt
    19561 ttggcggacg cgtgctgggc aacgataccc ccaaatacct gaactcgccg gaaacagaca
    19621 ttttccataa aggccgccag ctttacggtc tttatgaagc gcagcaggat aacgctgaac
    19681 ccaatcgtct gcttgtggtc gaaggctata tggacgtggt ggcgctggcg caatacggta
    19741 ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc cgatcacata caactgttgt
    19801 tccgcgcgac caacaatgtc atttgctgtt atgacggcga ccgtgcaggc cgcgatgccg
    19861 catggcgagc gctggaaacg gcgttgcctt acatgacaga cggtcgtcag ctacgcttta
    19921 tgtttttgcc ggatggcgaa gaccctgaca cgctggtacg aaaagaaggt aaagaagcgt
    19981 ttgaagcgcg gatggagcag gcgatgccac tctccgcatt tctgtttaac agtctgatgc
    20041 cgcaagttga tctgagtacc cctgacgggc gcgcacgttt gagtacgctg gcactgccat
    20101 tgatatcgca agtgccgggc gaaacgctgc gaatatatct tcgtcaggaa ttaggcaaca
    20161 aattaggcat acttgatgac agccagcttg aacgattaat gccaaaagcg gcagagagcg
    20221 gcgtttctcg ccctgttccg cagctaaaac gcacgaccat gcgtatactt atagggttgc
    20281 tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct tgagaatctg gatgaaaata
    20341 agctccctgg acttggctta ttcagagaac tggtcaacac ttgtctctcc cagccaggtc
    20401 tgaccaccgg gcaactttta gagcactatc gtggtacaaa taatgctgcc acccttgaaa
    20461 aactatcgat gtgggacgat atagcagata agaatattgc tgagcaaacc ttcaccgact
    20521 cactcaacca tatgtttgat tcgctgcttg aactgcgcca ggaagagtta atcgctcgtg
    20581 agcgcacgca tggtttaagc aacgaagaac gcctggagct ctggacatta aaccaggagc
    20641 tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagca atcgggaagc ccccgacagc
    20701 cgcactgaga ggcagcggca aatatataag tacgccctcg taattatcgt tggcggtaaa
    20761 caaccgttgg atttcagcgt taacggctga aggacatcgg gtcaatcgcc caacaccaac
    20821 ctcatgaaat aagtgtggat accgtcttat ggagcaaaac ccgcagtcac agctgaaact
    20881 tcttgtcacc cgtggtaagg agcaaggcta tctgacctat gccgaggtca atgaccatct
    20941 gccggaagat atcgtcgatt cagatcagat cgaagacatc atccaaatga tcaacgacat
    21001 gggcattcag gtgatggaag aagcaccgga tgccgatgat ctgatgctgg ctgaaaacac
    21061 cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt tccagcgtgg aatctgaaat
    21121 cgggcgcacg actgacccgg tacgcatgta catgcgtgaa atgggcaccg ttgaactgtt
    21181 gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa gacgggatca accaggttca
    21241 atgctccgtt gctgaatatc cggaagcgat cacctatctg ctggaacagt acgatcgtgt
    21301 tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc tttgttgacc cgaacgcaga
    21361 agaagatctg gcacctaccg ccactcacgt cggttctgag ctttcccagg aagatctgga
    21421 cgatgacgaa gatgaagacg aagaagatgg cgatgacgac agcgccgatg atgacaacag
    21481 catcgacccg gaactggctc gcgaaaaatt tgcggaactg cgcgctcagt acgttgtaac
    21541 gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc gctcaggaag agatcctgaa
    21601 actgtctgaa gtattcaaac agttccgcct ggtgccgaag cagtttgact acctggtcaa
    21661 cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa cgtctgatca tgaagctctg
    21721 cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc ctgtttaccg gcaacgaaac
    21781 cagcgatacc tggttcaacg cggcaattgc aatgaacaag ccgtggtcgg aaaaactgca
    21841 cgatgtctct gaagaagtgc atcgcgccct gcagaaactg cagcagattg aagaagaaac
    21901 cggcctgacc atcgagcagg ttaaagatat caaccgtcgt atgtccatcg gtgaagcgaa
    21961 agcccgccgt gcgaagaaag agatggttga agcgaactta cgtctggtta tttctatcgc
    22021 caagaaatac accaaccgtg gcttgcagtt ccttgacctg attcaggaag gcaacatcgg
    22081 tctgatgaaa gcggttgata aattcgaata ccgccgtggt tacaagttct ccacctacgc
    22141 aacctggtgg atccgtcagg cgatcacccg ctctatcgcg gatcaggcgc gcaccatccg
    22201 tattccggtg catatgattg agactatcaa caaactcaac cgtatttctc gccagatgct
    22261 gcaagagatg ggccgcgagc cgacgccgga agaactggct gaacgtatgc ttatgccgga
    22321 agataagatc cgcaaagtgc tgaagatcgc caaagagcca atctccatgg aaacgccgat
    22381 cggtgatgat gaagattcgc atctggggga tttcatcgag gataccaccc tcgagctgcc
    22441 gctggattct gcgaccaccg aaagcctgcg tgcggcaacg cacgacgtgc tggctggcct
    22501 gaccgcgcgt gaagcaaaag ttctgcgtat gcgtttcggt atcgatatga acaccgacca
    22561 cacgctggaa gaagtgggta aacagttcga cgttacccgc gaacgtatcc gtcagatcga
    22621 agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct gaagtgctgc gtagcttcct
    22681 ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca cccggcacta ggccctctgc
    22741 acaaacgcca ccttttcggt ggcgtttttt atcgcccacg cactaccagc gcctggtcca
    22801 gctcgcgata cgcttcaacc agtttctcca gtgaaacgcg acttaaacca ctgggatttg
    22861 gcagcaccca aatctgcgtc gaaccaatgg tgagcgtttg tttcccccac tgtgcaccgc
    22921 gctggctgaa tccctgttca tatgcttgtt tgcccagaat cgccaacgcc tgtggctgat
    22981 aatcttctat tttttcaatt agcttacgcc cgcctgcgtg cagctcctgc tttgaaactt
    23041 cattggcttg caccgttgga cggtctacca gtttggtgac gccacaacga taatccagca
    23101 gatgctgtgc ctcctgcggc ttcaactgac ggtcggtaaa cccggcctga tatatcacct
    23161 tccagaagcg atttgccgga tgagcaaagg gaaaaccagt cccggcggat gaaagcccag
    23221 ggttgatacc gcaaaacacg acccgtaacc ctggagccaa aatatcctca accatattta
    23281 ctcctttcta acatcaccag gaaaagtata acggattgaa aatacattgt ttataaaaac
    23341 agcaggcgcg cggtaatggc tggattgcga cacggagtta ctttataatc cgctaccatg
    23401 gccccttagc tcagtggtta gagcaggcga ctcataatcg cttggtcgct ggttcaagtc
    23461 cagcaggggc caccagatat agcaaaggct gacgagaaat cgtcagcctt tttcttttta
    23521 tatatcagtt actttgcgtg ccagtaagcc gctgcacgta cccgctgtgg atcatactgt
    23581 tccgcttcaa agcggcggct taaattctta acgactttgc cttcgccggt tatccagatg
    23641 aagtaatcat cggcagggat ttgcatctgc gccagacgcg catctaccgc ctgctcatca
    23701 tgtgccagcc attcgatatt aaaaccatca aggtgcgcga gataatcctg acaggcgtga
    23761 tcccgcacgc taaccagcgc gctaacttgc ggtttaacag caagtttgct caacgtttcc
    23821 aggcggcggc gcagtgcggg cattccggat tcatcgcaga catacagctg atacgcgtaa
    23881 tcctctggca tcaccagcga accgcgcgga cctgccaccg tgagtttatc gcccggttgc
    23941 gcctgcattg cccagctgct ggcgacgcca ccttcgtgaa taaagaaatc aatcgccagt
    24001 tcatggcgta gttcgtcata cagcggcgta tagtcacgcg acggtgggcg tggtccttcc
    24061 ggccagacga tgccctcttc cgttaccgtt ggcggcacaa agtgagcatc agattgagga
    24121 aagaagagtt tgctgtgatc gtcaaagccg cgcgatgtaa aaccgtccag cgcctcgccg
    24181 ccaaggacaa tgcgctgaaa accggcgcta atacgctcag cgcgtagcac agtcagttca
    24241 cggaagcgca gatcattgcg aacgcgctgc gggtagcggg gggagttatt cattgttatc
    24301 gccttcgtga tagtaatcag atatatctaa ataaatttcg caaatgataa tgattgttaa
    24361 tcatgataaa tgcaagcgat ttgtagaact gatatatctg aagtatgaga agacattcag
    24421 cgtcgtagca aggctgttgg atgcggcgtg aacgaattat ccggcctgga tgtgaggtgt
    24481 attcacgttt aaaaatcatt tttatacttg caaaagcgct catatcagat ataaattaga
    24541 tatatctaat taagcaaaag gaggctgata tgagccatca tcacgaaggg tgttgtaaac
    24601 atgaaggcca gccacgccat gaaggctgct gcaaaggtga gaagtcagaa catgagcact
    24661 gcggacacgg tcaccagcat gaacacggtc aatgctgcgg tggtcgccac ggtcgcggcg
    24721 gcggtcgtcg gcagcgtttc tttggtcacg gtgaattgcg tctggtgatt ctggatattc
    24781 tctcgcgcga tgacagccac ggttacgaat tgattaaagc gattgagaat ctaacccagg
    24841 ggaattacac cccaagcccg ggtgtcatct acccgacgct ggattttctg caggagcagt
    24901 cgctgattac catccgcgaa gaggaaggtg gtaagaagca gattgcgttg accgaacagg
    24961 gcgcgcagtg gctggaagaa aaccgcgaac aggtggagat gatcgaagaa cgcatcaaag
    25021 cgcgttgcgt tggtgctgca ctccgccaga acccgcaaat gaagcgggcg ctggataatt
    25081 ttaaagcggt gctggattta cgcgtcaacc agagcgatat cactgatgca caaataaaaa
    25141 agatcattgc ggtgatcgac cgcgccgctt ttgatattac gcaactggat taatcaccga
    25201 ttgccggatg tggcgtgccc tacccccccg taggcctgat aagatgcgcc gcatcgcatc
    25261 aggcattgtg cgccaaccgc cggatgcggc atatcgatta atgcagcacc gtcaccgcgt
    25321 cttccagtcg gctggcgcgg tgttttacca tcgccgacac ctgcgcactc tcttccacca
    25381 gctcggcatt tttctgggtg atgaggttaa gctcatccac tgcacgggtc aggctggaaa
    25441 gcccatcggc ctgttccagc gttgaatggc taatctgagc tatcaactgg gtgacgtttt
    25501 tcacctgtgc aacaatatct tccatcgtcc gtccggctgc gtgtacctgc tgcgaaccgg
    25561 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattggcgc
    25621 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccttgttcgc
    25681 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
    25741 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
    25801 tcttgatcac cgtggtcatc gcctccccgc cctgcactgc cgcattactg gcagtgatag
    25861 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
    25921 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
    25981 catcggtacc tttcgccagc gtctcactgc cgtttctgac actggacacc tggcttgaga
    26041 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
    26101 ccagctcatc gctgcgattc agatgctcaa cactattacg ctctccggtc gccaccttca
    26161 gtgcctgacg ggcgacattt tctatcgggc ggacaatctg ccattcaaaa caggcgcttg
    26221 ccaacaatac cactaacgca cagaggatat acgtcaccac cggggcagca acaaaccaaa
    26281 gcatgaccgc cagcaagata aacatcaggg tcatcacgcc acgcgcccgc cagcgaagcg
    26341 gtaatgaagg cagtttcccc agccagcctt tacgcaccac caggccttta tgaatacgct
    26401 tactggtacg tccggcgttc aacgctttgt acagcggctc caccgccgcg atctcttcat
    26461 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
    26521 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
    26581 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
    26641 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta tagccgctca
    26701 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
    26761 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
    26821 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
    26881 tgcggttatc tttaactgat taatttgatt tagatcgcaa tttgcgatta aagcataaat
    26941 ctaatccctt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
    27001 cccaaaaatc aggcagttgt tgccctgaaa gtgtgcattt gcgcagcaat cgccaaatcc
    27061 atacccgaca aaaaccgtgc aaaataacaa cagatgttaa caaatagcat taaatattgc
    27121 gcaaatgata accacatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
    27181 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
    27241 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
    27301 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
    27361 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
    27421 ttacggagcc gtagagtggc aagcgggagg tttaaatacg cttgtcgaca cccagggaca
    27481 ggagtttatc gactgccttg gaggttttgg aattttcaac gtggggcacc gtaatccagt
    27541 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
    27601 cgatccgtta cgggcgatgc tggcaaaaac acttgctgca ctgacgcccg gtaaactgaa
    27661 atacagcttc ttttgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
    27721 ggcttatcag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
    27781 taaatccctt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac catttatgcc
    27841 gctgctgcca gggttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggccct
    27901 tagcgagtgc aaaaaaaccg gtgatgatgt tgctgcagtg atccttgagc cgattcaggg
    27961 cgaaggtggc gtcattctgc cgccgccggg atatctcacc gccgtgcgta agctgtgcga
    28021 tgagttcggc gcactgatga tcctcgatga agtgcaaacc ggtatggggc gcacgggcaa
    28081 gatgttcgcc tgcgagcatg agaacgtaca gccggacatc ctgtgtctgg caaaagccct
    28141 cggcggcggc gtgatgccga ttggcgcaac catcgccact gaagaggtgt tttccgttct
    28201 gttcgacaac ccgttcctgc ataccactac ctttggcggc aacccgctgg cctgtgcggc
    28261 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgaacaaaa
    28321 aggcgatatg ttgctggacg gtttccgtca attggcgcgg gaatatcccg atctggtaca
    28381 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
    28441 taactttgcc agcgagatgt tccgccagcg cgtcctggtg gccggaacac tcaacaacgc
    28501 taaaacgatc cgcattgaac caccactgac actgaccatt gaacagtgtg aactggtgat
    28561 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
    28621 agtcgaatgg cgatacgacg tcgccattcg atctttttat acaatacgta cgcccgcagg
    28681 catcatccgc tccggcgtta acaacacgct ttcactgccg tcatcggttt ccgcacacaa
    28741 cagcatgcat tccgacgttt caccgcgcat tttcgctttt tgcagattac ataagaccac
    28801 caccgttttt tccatcagct cttcttcgct gtagtacggc accaggctgg tcacggtttg
    28861 cagcgttttt tcacccacat caacctgtac gatgtacagc ttgtcggcgt tttcatgacg
    28921 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
    28981 ggtttccatt gtttcctccc ttcgggtaaa attttactaa actatagcaa aagtttttct
    29041 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgcaag ccgctgtgtt
    29101 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgatc cgataagcac
    29161 ttcctctaca atggaggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacactaa
    29221 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
    29281 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
    29341 tggagtacaa gaccagtagc gcccgtaaac tccagacagg cgcagttaac caacaccata
    29401 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
    29461 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
    29521 aacacaacgg cttaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
    29581 cgcccgccct gcgcgccgcc gcctgtgcgt tgaccgacaa tatctgtttt atcgactttc
    29641 acgaacctag cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
    29701 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
    29761 agccaggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
    29821 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggc tatgaactgg
    29881 caaaacagat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
    29941 ttgctatcgg cgtactgcgg gcgattcatg aacgtggcct gaacatccca caggatattt
    30001 cacttatcag cgttaatgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
    30061 tgcgcatcca ttccgaaatg atgggaagcc agggcgttaa cctggtgtat gaaaaagccc
    30121 gcgatggtcg cgcgctgccg ctgttagtct tcgttcccag caaattaaaa ctgcgcggca
    30181 cgacccgtta aatcccctta cacactgtcc ggcaatcgtt tttgccggac agtgctgccg
    30241 tttattttcg tgatccagtt aaagtaaatg catttacccg ctacttttta gtaaaaattt
    30301 tactaaactc cccagcaatt acacaaacta ccatcaccat aaatggttcc gatttctctc
    30361 taccgggagg ccctatgaat cgctgggaaa acattcagct cacccacgaa aaccgacttg
    30421 cgccgcgtgc gtactttttt tcatatgatt ctgttgcgca agcgcgtacc tttgcccgcg
    30481 aaaccagcag cctgtttctg cccttaagcg gtcagtggaa tttccacttt tttgaccacc
    30541 cgctgcaagt gccagaagcc ttcacctctg agttaatggc tgactggggg catattaccg
    30601 tccccgccat gtggcaaatg gaaggtcacg gcaaactgca atataccgac gaaggttttc
    30661 cattccccat cgatgtaccg tttgttccca gcgataaccc aaccggtgcc tatcaacgta
    30721 ttttcaccct cagcgacggc tggcagggta aacagacgct gattaaattt gacggcgtcg
    30781 aaacctattt tgaagtctac gttaacggtc agtatgtggg tttcagcaag ggcagtcgcc
    30841 tgaccgcaga gtttgacatc agcacgatgg ttaaaaccgg cgacaacctg ttgtgtgtgc
    30901 gcgtgatgca gtgggcggat tccacctacg tggaagacca ggatatgtgg tggtcggcgg
    30961 ggatctttcg cgatgtttat ctgatcggaa aacaactaac acatattaac gatttcaccg
    31021 ttcgtaccga ctttgacgaa gcctattgcg atgccacgct ttcctgcgaa gtggtgctgg
    31081 aaaatctcgc cgcctcccct gtcgtaacga cgctggaata taccctgttt gatggggaac
    31141 gcctggtgca cagcagcgcc attgatcatt tggcaattga aaaactgacc agcgccagct
    31201 ttgcttttac tgtcgaacag ccgcagcaat ggtcagcgga atccccttat ctttaccatc
    31261 tggtcatgac gctgaaagac gccgacggca acgttctgga agtggtacca caacgcgttg
    31321 gcttccgtga tatcaaagtg cgcgacggtc tgttctggat caataaccgt tatgtgatgc
    31381 tgcacggcgt caaccgtcac gacaacgatc atcgcaaagg ccgcgccgtt ggaatggatc
    31441 gcgtcgagaa agatctccag ttgatgaagc agcacaacat caactccgtg cgtaccgctc
    31501 actacccgaa cgatccgcgt ttttacgaac tgtgtgatat ctacggcctg tttgtgatgg
    31561 cggaaaccga cgtcgaatcg cacggctttg ctaatgtcgg cgatatcagc cgtattaccg
    31621 acgatccgca gtgggaaaaa gtctacgtcg agcgcattgt tcgccatatt cacgcgcaga
    31681 aaaaccatcc gtcgatcatc atctggtcgc tgggcaatga atccggctat ggctgtaaca
    31741 tccgcgcgat gtaccacgcg gcgaaggcgc tggatgacac gcgactggtg cattacgaag
    31801 aagatcgcga tgctgaagtg gtcgatatta tttccaccat gtacacccgc gtgccgctga
    31861 tgaatgagtt tggtgaatac ccgcatccga agccgcgcat catctgtgaa tatgctcatg
    31921 cgatggggaa cggaccaggt gggctgacgg agtaccagaa cgtcttctat aagcacgatt
    31981 gcattcaggg acattatgtc tgggaatggt gcgaccacgg gatccaggcg caggatgaca
    32041 acggcaatgt ctggtataaa ttcggcggcg actacggcga ctatcccaac aactataact
    32101 tctgtcttga tggtttgatc tattccgatc agacgccggg accgggcctg aaagagtaca
    32161 aacaggttat tgcgccggta aaaatccacg cgctggatct gactcgcggc gaactgaaag
    32221 tcgaaaataa actgtggttt accacgcttg atgactacac cctgcacgca gaggtgcgcg
    32281 ccgaaggtga aacgctcgcg acgcagcaga ttaaactgtg cgacgttgca ccgaacagcg
    32341 aagccccctt gcagatcacg ctgccgcaac tggacgctcg tgaagcgttt ctcaacatta
    32401 cggtgaccaa agattcccgc acccgctaca gcgaagccgg gcattctatc gccacttatc
    32461 agttcccgct gaaggaaaat accgcgcagc ctgtaccttt cgcaccgaat aatgcgcgtc
    32521 cgctgacgct ggaagacgat cgtttgagct gcaccgttcg cggctacaac ttcgcgatca
    32581 ccttctcaaa aatgagtggc aaaccgacat cctggcaggt gaatggcgag tcgctgctga
    32641 cccgcgagcc aaagatcaac ttcttcaagc caatgatcga caaccacaag caggagtacg
    32701 aagggctgtg gcagccgaat catttgcaga tcatgcagga acatctgcgc gactttgccg
    32761 tagaacagag cgatggtgaa gtgttgatca tcagccgcac ggttattgcc ccaccggtgt
    32821 ttgacttcgg gatgcgctgc acctacatct ggcgcatcgc tgccgatggc caggttaacg
    32881 tggcgctttc cggcgagcgt tacggcgact atccgcacat cattccgtgc atcggtttca
    32941 ccatggggat taacggcgaa tacgaccagg tggcgtatta cggtcgtgga ccgggcgaaa
    33001 actacgccga cagccagcag gctaacatca tcgatatctg gcgcagcacc gtcgatgcca
    33061 tgttcgagaa ctatcccttc ccacagaaca acggcaaccg tcagcatgtc cgctggacgg
    33121 cactgactaa ccgccacggc aatggtctgc tggttattcc gcagcgccca attaacttca
    33181 gtgcctggca ctacacccag gaaaacatcc acgctgccca gcactgtaac gagttgcagc
    33241 gcagtgatga catcaccctg aatctcgacc accagctgct tggcctcggc tccaactcct
    33301 ggggcagcga ggtgctggac tcctggcgcg tctggttccg tgacttcagc tacggcttta
    33361 cgttgctgcc gatttctggc ggagaagcta ccgcgcaaag cctggcgtcg tatgagttcg
    33421 gcgcagggtt cttttccacg aatttgcaca gcgagaataa gcaatgagga tcatcgataa
    33481 cttagaacag ttccgccaga tttacgcctc tggcaagaag tggcaacgct gcgttgaagc
    33541 gattgaaaat atcgacaaca ttcagcctgg cgtcgcccac tccatcggtg attcattgac
    33601 ctaccgtgtg gaaaatgact ccgcgaccga tgcgttattt accgggcatc gacgctattt
    33661 tgaagtgcat tactacctgc aagggcagca aaaaattgaa tatgcgccga aagagacatt
    33721 acaggtagtg gaatattatc gtgatgaaac tgaccgtgaa tatttaaaag gctgcggaga
    33781 aaccgttgag gtccacgaag ggcaagtcgt tatttgcgat atccatgaag cgtatcggtt
    33841 tatctgc
//
LOCUS       DAOTRK010000040        33653 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.040, whole genome shotgun sequence.
ACCESSION   DAOTRK010000040 DAOTRK010000000
VERSION     DAOTRK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.040"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(94..2139)
                     /gene="dcp"
                     /locus_tag="QIE41_001843"
     CDS             complement(94..2139)
                     /gene="dcp"
                     /locus_tag="QIE41_001843"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDS3530736.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQYNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            2276..3022
                     /gene="ydfG"
                     /locus_tag="QIE41_001844"
     CDS             2276..3022
                     /gene="ydfG"
                     /locus_tag="QIE41_001844"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDS3530737.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3111..3797
                     /gene="ydfH"
                     /locus_tag="QIE41_001845"
     CDS             3111..3797
                     /gene="ydfH"
                     /locus_tag="QIE41_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDS3530738.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            3974..4177
                     /gene="ydfZ"
                     /locus_tag="QIE41_001846"
     CDS             3974..4177
                     /gene="ydfZ"
                     /locus_tag="QIE41_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDS3530739.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4213..5673)
                     /locus_tag="QIE41_001847"
     CDS             complement(4213..5673)
                     /locus_tag="QIE41_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDS3530740.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYHHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5762..7129)
                     /gene="ydfJ"
                     /locus_tag="QIE41_001848"
     CDS             complement(5762..7129)
                     /gene="ydfJ"
                     /locus_tag="QIE41_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HDS3530741.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKLAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(7187..8206)
                     /locus_tag="QIE41_001849"
     CDS             complement(7187..8206)
                     /locus_tag="QIE41_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDS3530742.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHMFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(8218..9432)
                     /gene="rspA"
                     /locus_tag="QIE41_001850"
     CDS             complement(8218..9432)
                     /gene="rspA"
                     /locus_tag="QIE41_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDS3530743.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(9638..9964)
                     /locus_tag="QIE41_001851"
     CDS             complement(9638..9964)
                     /locus_tag="QIE41_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDS3530744.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRA"
     gene            10099..10440
                     /locus_tag="QIE41_001852"
     CDS             10099..10440
                     /locus_tag="QIE41_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDS3530745.1"
                     /translation="MKITLSKRIGLLAFLLPCAMALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            10475..11035
                     /gene="speG"
                     /locus_tag="QIE41_001853"
     CDS             10475..11035
                     /gene="speG"
                     /locus_tag="QIE41_001853"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDS3530746.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(11038..11748)
                     /locus_tag="QIE41_001854"
     CDS             complement(11038..11748)
                     /locus_tag="QIE41_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDS3530747.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            11856..12161
                     /locus_tag="QIE41_001855"
     CDS             11856..12161
                     /locus_tag="QIE41_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDS3530748.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            12360..14786
                     /locus_tag="QIE41_001856"
     CDS             12360..14786
                     /locus_tag="QIE41_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDS3530749.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGNDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            14847..17270
                     /locus_tag="QIE41_001857"
     CDS             14847..17270
                     /locus_tag="QIE41_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDS3530750.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIHLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            17281..17898
                     /gene="dmsB"
                     /locus_tag="QIE41_001858"
     CDS             17281..17898
                     /gene="dmsB"
                     /locus_tag="QIE41_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDS3530751.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            17900..18754
                     /locus_tag="QIE41_001859"
     CDS             17900..18754
                     /locus_tag="QIE41_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDS3530752.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VTIIGKMPQALGKLWLLVSMALGVIFVWMMTGVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            18797..19411
                     /gene="dmsD"
                     /locus_tag="QIE41_001860"
     CDS             18797..19411
                     /gene="dmsD"
                     /locus_tag="QIE41_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDS3530753.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMQQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKSLFR"
     gene            19569..20861
                     /gene="clcB"
                     /locus_tag="QIE41_001861"
     CDS             19569..20861
                     /gene="clcB"
                     /locus_tag="QIE41_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDS3530754.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFVQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(20814..21509)
                     /gene="bioD"
                     /locus_tag="QIE41_001862"
     CDS             complement(20814..21509)
                     /gene="bioD"
                     /locus_tag="QIE41_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDS3530755.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(21634..22854)
                     /gene="mlc"
                     /locus_tag="QIE41_001863"
                     /gene_synonym="dgsA"
     CDS             complement(21634..22854)
                     /gene="mlc"
                     /locus_tag="QIE41_001863"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDS3530756.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEEAQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(22989..23882)
                     /locus_tag="QIE41_001864"
     CDS             complement(22989..23882)
                     /locus_tag="QIE41_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3530757.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            23989..25242
                     /locus_tag="QIE41_001865"
     CDS             23989..25242
                     /locus_tag="QIE41_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3530758.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            25639..25974
                     /locus_tag="QIE41_001866"
     CDS             25639..25974
                     /locus_tag="QIE41_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDS3530759.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            26067..26150
                     /locus_tag="QIE41_001867"
     CDS             26067..26150
                     /locus_tag="QIE41_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530760.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            26250..27071
                     /locus_tag="QIE41_001868"
     CDS             26250..27071
                     /locus_tag="QIE41_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDS3530761.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDRLDQLSQK"
     gene            complement(27110..27439)
                     /gene="mdtI"
                     /locus_tag="QIE41_001869"
     CDS             complement(27110..27439)
                     /gene="mdtI"
                     /locus_tag="QIE41_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDS3530762.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(27426..27791)
                     /gene="mdtJ"
                     /locus_tag="QIE41_001870"
     CDS             complement(27426..27791)
                     /gene="mdtJ"
                     /locus_tag="QIE41_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDS3530763.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAFKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            28203..29237
                     /gene="tqsA"
                     /locus_tag="QIE41_001871"
     CDS             28203..29237
                     /gene="tqsA"
                     /locus_tag="QIE41_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDS3530764.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(29262..30650)
                     /gene="pntB"
                     /locus_tag="QIE41_001872"
     CDS             complement(29262..30650)
                     /gene="pntB"
                     /locus_tag="QIE41_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDS3530765.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTSIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(30661..32193)
                     /gene="pntA"
                     /locus_tag="QIE41_001873"
     CDS             complement(30661..32193)
                     /gene="pntA"
                     /locus_tag="QIE41_001873"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDS3530766.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            32717..>33653
                     /locus_tag="QIE41_001874"
     CDS             32717..>33653
                     /locus_tag="QIE41_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDS3530767.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADL"
BASE COUNT         8565 a         8680 c         8067 g         8341 t
ORIGIN      
        1 attagcggtg aggtttgccc tcgcccagct gctgcaatta ctactctaac caggctggcc
       61 tggcgatttc tccaggccag ccattggtgg tgtttatacg ttcaagccac gatgttgcag
      121 catcggcata atctgaggtg ccttaccgcg ccattgtcga tacaggcgtt ccagatcctc
      181 gctgttacct ctggaaagga tcgcctcgcg aaaacgcagc ccattttcac gcgttaatcc
      241 gccctgctca acaaaccact ggtaaccatc atcggccaac atttgcgtcc acagataagc
      301 gtaataacct gcggcatatc cgccaccaaa aatatgggcg aaataactgc tgcgatagcg
      361 tggcggtata gcaggaagat ccatattttc cgccaccagc gcccgcaatt caaaatcatc
      421 gacatcctgc attgcttcgt tttcttccag gcaatgccag cgcatatcga gaagtgcggc
      481 gctaagcagt tcgctcatct catacccttt gttgaacagg ctggcattac gcattttctg
      541 ttgcagttcg tcaggcattg ctgccccgct ctgataatgc cgggcgtagc gagcgaatac
      601 ctgcggatgc gttgcccagt gttcattgat ttgcgacgga aattcgacaa aatcacgcgg
      661 cgtgttggtg ccggaaagcg tggcataacg ctggcgggca aaaaggccgt gcagcgtatg
      721 accaaattca tggaataagg ttattacatc atcccagagt aacaacgcag gctcaccggc
      781 agcgggtttc tgataattgc aaacgttata aattaccggg tgcgtttcat taagcgttga
      841 ttgctcaaca aaattgccca tccatgcacc gccgcttttt gaatcacggg cgaagaaatc
      901 accgtaaaat aacgccagcc ccacgccatt atgatcaaaa atttcccaca cccgaacgtc
      961 aggatggtag acaggaatat caaaacgttc gacaaactta ataccgaaga gctgattcgc
     1021 ggtccagaat acaccttcat tcaacaccgt gtttaattca aaatatggct tgagctgcgc
     1081 ctcatcaaga tcaaatttct cccgccgtac ctgttcggca taaaatgccc agtcccacgg
     1141 ctgcgcgcta aacccgccct gctgcttatc aataaccgcc tgtatggagg ctaattcatc
     1201 gctcgcacgt tgacgcgccg ctggaacaat ttcccgcata aagttgagtg ctgcttctgg
     1261 cgtttttgcc atctgatcgg cgattttcca tgcggcataa tgagggaaac caagcagttt
     1321 cgcctgctgc gcacggatct ccaccagacg ttgaatgata gcgcgggtat cattggcatc
     1381 atttttttcc gctcgcgtcc agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg
     1441 catttcggca agcgccggtt gttgggtggt attcagcaac ggaatcagcc atttgttatc
     1501 cagacctttc tcgcgagccg cctctgccgc cagagcaatc tcttgctcac tcattcctgc
     1561 cagctgcgcg aaatcgttca caaccagacc gccggattta tttgctgcca gtaaccgctg
     1621 gttatactgg ctggtcaggg tcgcagcttc tgtattcagt acttttaatt ttgctttatc
     1681 agcttgcgca agtttggctc cggcaaggac aaaacgttga tgaatcacct ccaccaggcg
     1741 gatggattca ctatcaagcc ccagggattc tcggcgctgc cagacagcat ctacccgcgc
     1801 gaataattca ccgttcagat agatatcatt agccagttcc gccagttcag cggaaaactg
     1861 ttcatcaaga cgctgtaatt catcattggt atgcgccgca gtcatcgcaa aaaagacgct
     1921 ggtaacgcgg gtaagtaatt ctccgctttg ttccagtgcc agaatagtat tgttgaaatc
     1981 aggcgtttgc gggttaagcg cgatggcagc aatttctgcc cgcttttgca gcattccctc
     2041 atcgaatgcc gggcgatagt gatgattggc aatttgatca aaatggggag ccagatacgg
     2101 cagtgtgctt tgcacaagga aaggattcat tgttgtcatt ttcttctcct gaacgcgagg
     2161 tgttccatag cgtaggctta ctgataacgg agtgcaatct tgcaaaccag tattacccgc
     2221 tcttaagcat cccgtgctat gttattgaca cacaaaagcg ttgaggaaca gtgagatgat
     2281 cgttttagta actggagcaa cggcaggttt tggtgaatgc attactcgtc gttttattca
     2341 acaagggcat aaagttatcg ccactggccg tcgccaggag cggttgcagg agttaaaaga
     2401 cgaactggga gataatcttt atatcgccca actggacgtt cgcaaccgcg ccgctattga
     2461 agagatgctg gcatcgcttc ctgccgagtg gtgcaatatt gatatcctgg taaataatgc
     2521 cggtctggcg ttgggcatgg agcctgcgca taaagccagc gttgaagact gggaaacgat
     2581 gattgatacc aacaacaaag gcctggtata tatgacgcgc gccgtcttac cgggtatggt
     2641 tgaacgtaat catggtcata ttattaacat tggctcaacg gcaggcagct ggccgtatgc
     2701 cggtggtaac gtttacggtg cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg
     2761 tacggacctg catggtacgg cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg
     2821 caccgagttt tccaatgtcc gctttaaagg cgatgacggt aaagcggaaa aaacctatca
     2881 aaataccgtt gcattgacgc cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct
     2941 gcctgctcac gttaatatca ataccctgga aatgatgccg gttacccaaa gctatgccgg
     3001 actgaatgtc caccgtcagt aatttttata cccggcgtaa ctgccgggtt attgcttgtc
     3061 acaaaaaagt ggtagactca tgcagttaac tcactcacaa gcaagaacga atgaccgtcg
     3121 aaacgcaact taatcccaca cagcctgtca atcagcaaat ttatcgtatc cttcgtcgcg
     3181 acattgtcca ttgcctgatt gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc
     3241 gtttcaatgt gtcacgccag ccggttcgtg aagcctttat taaactggcg gaaaacggcc
     3301 tgatccaaat tcgtccgcag cgtggcagct acgtcaacaa aatttccatg gcccaggtgc
     3361 gcaacggcag ttttatccgt caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca
     3421 tgattaccga aagccagtgc taccaactgg aacaaaatct tcaccagcaa cgcattgcca
     3481 ttgagcgcaa gcaactggat gatttttttg aacttgatga caacttccat caactcctga
     3541 cccagattgc cgactgtcaa ctggcgtggg ataccattga gaacctgaaa gcgaccgttg
     3601 atcgcgtgcg ctatatgagt ttcgaccacg tttctccacc agaaatgctg ttacgccagc
     3661 atcttgagat tttctctgcc ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga
     3721 cgcaacattt gcaggaaatc agcgaatccg tgcgccagat ccgtcaggaa aacagcgact
     3781 ggtttagcga agagtaattc atttcctctc atcccatccg ggatgagagt ttttccctcc
     3841 gtcatatggc tcatgcatgc atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt
     3901 tcactcgaca ggagtattta tattgcgccc gttacgtggg cttcgactgt aaatcagaaa
     3961 ggagaaaaca cctatgacga cctacgatcg taaccgtaac gcaatcacca ctggcagccg
     4021 tgttatggtt agcggcaccg gtcacactgg caagatcctg tcgattgata ctgaaggtct
     4081 gaccgctgag caaatccgcc gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact
     4141 ggcacctctg gacctgattc gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg
     4201 gcattgcttt ttttacttca cggaatattt cgccacggtc gcttttgcac catgcgctaa
     4261 taaagacaag tacgcttccg tcacttttgc agtaaacaag ctattgtctg gcaaatcatc
     4321 accaaagatc gccttaatcg ccagcaatga ctggacgcgc gctttccctt cggcactact
     4381 ttgtacagcc ttctgaataa caggtaacag tgggtcactg atttctatcg gatttccctg
     4441 ttcatcaaca ccacccacat aacgcatcca acctgcgacg cccagcgcca gcagatcgaa
     4501 cttgctgtca tgcgccagat gccagcgaac agaatctaac atccgctgtg gcaatttctg
     4561 gctgccatcc atcgcaatct gccaggttcg atgacgtagc gccgggttgc tatagcgttc
     4621 aatcaatcgg ttagcgtaat cttgcaaatc aacgccctgc actttcaacg tcggcgcttg
     4681 ttcctgcaac atcaaggcat acgctgcatg atgataatgt tcatcttcca tgcagtcatt
     4741 aatgtgctga tatccggcaa gataccccag atacgccagg aatgaatgac tgccgttgag
     4801 catgcgcaac ttcatctctt cataaggcag cacatcacta accagttcgg ctcccgcttt
     4861 ttcccattcc ggacgtccgg caacaaagtt atcttcgata acccactggc ggaaaggttc
     4921 acaggcaacg cccgcaggat cgcgcacacc ggtaagttgt tcgattttcg ccagcgtatc
     4981 ctccgtcact gcgggcacaa tacggtccac cattgttgat gggaaagtca cgttttcttc
     5041 gatccattgt gccagtttta catcaacagc ttgcgcgtag gaagtgacaa cgtcacgcat
     5101 aacatgaccg ttttctggca tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc
     5161 cgctttacgg cgagccagcg cttcgacaat cacccctgtt gcagttttcg gctggtgggg
     5221 attttgtacg tcggcaacga ccatcgggtg atcgagcatt aactgtccgg tcgccggaga
     5281 gtggaaatac cctttttcgg tgattgtcag agagacaatc gcgatttgtg gttcacacat
     5341 cgcagccaac acggtttcta agccatcaat ctgtacgtgc aaggcttttt taacgacgcc
     5401 aacgacgcga gccgtccacg catcggccga catttccgca acggtataaa gattatcttg
     5461 ctgttgtaaa tcggcaattt gctgttcacc gccgattaaa ttgacctcat aatatcccca
     5521 gtcactgaaa tgttccgtag caagaatatc ggcatacacg ccctgatgcg cacggtgaaa
     5581 tgcaccaaag cctaaatgaa caattcttgg ggccaggtta ttacgatcat aaacagggag
     5641 cgtcgctttt gctgataaca aattatttcc cataacaatt ccttaaatat aaatatggca
     5701 agctacatgt tttgttatat gaataaaaat cccctctccg gtaagagaag ggattgagga
     5761 tttaaagact tctggaaggt tgcgcagctc tgacaacacg cggttgatct tccgcagcgt
     5821 cttccagcgc acttaaatca cggtctttca cttctggcat tttcagcgca gagattaaac
     5881 caatcactga atatgccatg atcataatgg cgatcggata ccaggattcc gtcatggtgc
     5941 agaaaatacc cgccaggata ggaccaaaac cggaagcgat aagaccacca atttctttag
     6001 aaatagccat ccgggtaaag cggtttttac agccgaacat ttctgccatg gtaatgtttt
     6061 ccagagcaaa taatcccagc accgcacagt tatgaatcac aatcagtgca accataatgg
     6121 tgctcggggc atagctttta tctacgatga tagaaagcat tggccacgcc agcacaatcg
     6181 cagaggtatt cataataata tacgggatcc ggcgaccaat tttatcggat aaccaaccaa
     6241 ggaacggaat ggtcataaag ccgagaatcg aactgatcat caatgcatct gttggaattg
     6301 ctttgttaaa caataacgtc tgcactaaat agcctgcaag gaaagtctga attaaaccgg
     6361 agttacccgc ctgaccaaaa cgcagccctg ttgccagcca gaaggatttg ctctggaaca
     6421 tgctaccagc aggtgcaagt tttgctgtcg gttggttgct gtcgttaacc ttctcaaaga
     6481 ccgggctttc tttcagattc atacgcaacc agatagcaaa gaccatcacg acaacgctcg
     6541 ccaggaacgg tatacgccat ccccacgcca gcagttcctc tttactgaga atgaagaaca
     6601 taaaggccca gattgccgtt gcgctcaagg ttccgcagtt agtccccata gcaacaaatg
     6661 aggagataat tccgcgctta ccttttggtg catattccgc cagcatcgtg ccggcaccgg
     6721 aaatttccgc gcctgccccc agcccctgaa taatacgcag cgtcaccagc aaaattggcg
     6781 caaacacacc aatttgtgca taggtcggca gcacaccaat taaggtggta cagatcccca
     6841 tcatagtgat ggtaataaag agcacttttt tacgcccgat tctgtcgccc attttgccga
     6901 aaataaatgc tccgacaata cgcgccacat aacctgcacc gtaggttccc attgccagaa
     6961 ttaacgccat tgccgttgat gattcaggaa aaaatatttc atgaaacact aacgctgcgc
     7021 cgagcgaata taactggaaa tccataaatt caagcgcagt gcccagccat cccgatactg
     7081 ctgctttcac caaatcctta gtgcttcttt cgtgtttttc tattgtcata atggttatct
     7141 ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta ttggtattat tcagaaaaag
     7201 tgagtaagac tttgcagcaa tgtttttgat cctgttcaaa taaactgatg gcatcagcaa
     7261 catgctggaa atcaaacata tgggtaatta atttttctgg tttaattaac cctttactta
     7321 accagtcgat aacgaccggg aatttatttg catttaagcg tgaagagaaa atagagagtt
     7381 cttttccggt aattccttgc tgaatcactt cagacggttc actggagaag cccatcaata
     7441 caatacgtgc cgctggagaa gccagcgtta cggcctcttt caggatagaa ggatgacaag
     7501 ccgcatcgat aattaatgtc ggctttatgc ctttttcagc gaaaatctcg ccaagcggtg
     7561 tctggctgtt attaatcgcc cagtcagccc cgctctcttt cgctttttcc agtcgttcat
     7621 caatgcgatc ggcaacaatc acatttttaa cgttatagac gccttttaat acctgaacga
     7681 tcgtcaggcc gattggaccg gcaccgtaaa ccagaacggt atcattttca gtcggttgac
     7741 catgaccggt tacgttcgcc gcaatggtaa aaggttcgat catcaccgca tattgatcgg
     7801 ccactgcttc aggaattttc cacgcatttt ttgccggaac cacggcatat tcactgaaac
     7861 caccgtcagc gtgcacacct aatacagcca gtgtcgtaca aacgttcggc ttacctatag
     7921 agcacggata gcaatgccca cagctgacca ccggatcgac agcaacgcgt tcaccgactc
     7981 tggcgctttc cacgccttca cccactgcat caatgacgcc aaagaattca tgaccaatga
     8041 cgcgcggata tttcgcaaaa ggattatgcc cacgataaat atggctatct gaaccacaaa
     8101 ttccggcaag tttcactttt actcgtactt cacccgctga cggggtgggt atttcgcgtt
     8161 cgataattgc cagttgattc ggtttttcaa ttaaaatgct tttcattatc ttactcctta
     8221 ccagttccac agcgtgccat cttccagacg tgcgactggc agataagcag gttcataggg
     8281 atatttcgcc gccagctttt catcgaattc gatgccaaga cccggtttgt ctcccggatg
     8341 catatagccg ttatcgaaag tccagttgtg cgggaagact tcaagcattt gttcggaata
     8401 acccatgtac tcctggacac cgaaattggg gacccacaga tcaaagtgca gcgccgcagc
     8461 catgcagact ggtgacaaat cggaaggacc gtgtgagcca gtacgtacct gatacaacga
     8521 agcaaaatcg gcaatccggc gcataccggt aattccgcct gcatgggtca gcgtggtgcg
     8581 gatataatcg ataagttgct cttcaatcag ttgcttgcag tcccagatgc tgttgaagac
     8641 ttcacccacc gcgatgggtg tgacggtatg ttggcgaatg agacggaagc attcctggtt
     8701 ttccgcaggc gtcgggtctt ccatccagaa catgcgataa tcttcaatgc ttttaccaaa
     8761 gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca tgcagcaaat gttcattaaa
     8821 accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg aaatcgaggt atttctccgt
     8881 cgaccacagc tgctcttccg gccactgtcc tttggttgcg ggttcataag ccagaccttt
     8941 acctttcgac atgccgtagg tggttttcat accagggatt ccgcactgca cgcggatggc
     9001 tttgaatccc agctcctgat gacgagcata atcatccaga gcttcatcaa tactgtgacc
     9061 ggtggtatgg caataaacca tcaccccttc acgagacgcg ccgccgagta actggtaaag
     9121 cggcatgttg gcagctttgg ctttaatatc ccacagcgcc atatcaaccg ctgaaatggc
     9181 cgacatcgta accggaccgc gacgccagta agcaccttta tagaaaaact gccagatatc
     9241 ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa aggtgatcct gcaaataaga
     9301 ggccacggaa agctcacgtc cattgagggt ggcatcccca aggcccgtaa taccgtcctc
     9361 agtggtgatt tttaatgtga cgaaattacg ccccggacag gtaacaaaaa cttcagcctt
     9421 tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga tgcatgaaat ctacgcaact
     9481 gagctactac catacaagta taaagatcaa aaaaagccgg agtgatcaca aaaaaaggcg
     9541 tatatttgcg ccgtgaatgg ttgacaaaag atgaaataga atatcttttg tcagctgaca
     9601 cttccttgta tcttattgat aaaatggatt tatgtcctta cgcgcgcccc cagcccgcaa
     9661 caatgatcaa catgccgcaa agcgcaatca acgcccccgt ccagtcataa agactcagtt
     9721 tcacgccatc cacaacacgc agccaaatca acgccgtgca gacataaacg ccaccataag
     9781 ccgcgtaaac acgcccactc gccgcaggat gcaacgttaa caaccagaca aacagtgcca
     9841 gagaaatccc cgccggaagc aacagccaga tactggcgtt tcgttttaac cacaaccagg
     9901 gcagaaagca tccaataatt tcacacagcg cagtagcaaa aaatagtaac gttgttttaa
     9961 tcatctttgt cacttattga catcatgtat agttatagga cgacataata tcatcaatat
    10021 aaacatcctc ctggtacgtt ttgcgttcgc agtggatggt gtagaatcac ctttaatcat
    10081 tcatacaggg aatgaattat gaaaattact ctcagcaaac gaattggcct gctcgctttt
    10141 ctgctgcctt gcgcaatggc attgagcaca actgttcatg ccgaaactaa caaactggtg
    10201 attgagtctg gcgacagtgc acaaagccgc cagcacgccg ctatggaaaa agagcaatgg
    10261 aatgacacgc gcaatctgcg tcagaaagtg aataaacgca ctgaaaaaga gtgggataaa
    10321 gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa gcgccaacat caatgcctac
    10381 tgggagccaa atactttgcg ctgcctggac cgtcgaactg gccgcgttat taccccctaa
    10441 cctgttattg atttaaggaa tgtaaggaca cgttatgcca agcgcccaca gtgttaagct
    10501 acgcccgctg gagcgtgaag atttacgcta tgtacatcaa ctcgacaata acgccagtgt
    10561 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtacga
    10621 taagcatatt cacgatcaga gcgaacggcg ctttgtggtg gaatgtgacg gcgaaaaagc
    10681 cggtctggtg gagctggtgg aaattaacca tgttcatcgc cgcgcagaat ttcagataat
    10741 tatctccccg gaatatcagg ggaaaggtct ggcaacccgt gctgcaaaat tagcaatgga
    10801 ctatggcttc accgttctca atctctataa gctgtatctg atcgtcgata aagagaatga
    10861 aaaagcgatt cacatttacc gcaagcttgg ctttacggtt gaaggtgaat tgatgcatga
    10921 gttctttatt aatggtcaat atcggaatgc cattcgcatg tgcatattcc agcatcagta
    10981 tctggcagag cacaaaacac cgggtcagac tctcctgaag ccgaccgcac aatagcatta
    11041 ataataatcg atagtatttt tgatggtgta aacccgttcg acggcgggtt ttactccttc
    11101 atcaacaatg attagctgac agtccaccgg attggcgtga ctgtcatatt cacagctctg
    11161 ttttacatta ccaactcgtt gattattcag taaagtaacc gctgtgtaat ctaatttttt
    11221 gatcggatcc gttgatggcg tagcgctgac agataatgtt ttgtcgttac ttttcgtggt
    11281 tttacccagc ggataaccct gatcatcata gcgatattcc atctgcatct gtttgctgct
    11341 ggctttaatc acaaagccat tatcatcggt ttcccaactc acccctgcag aaggtaattc
    11401 tgctagctgg cattttccct gcaaacgaac tcttttctcc agtgtctggg catcacggta
    11461 ataattggcg tccagtacca gggcaaccac ggtattattt tccagatcca gtaattcgag
    11521 tgaatcaaaa cagccttctt ccgacaaagt ccccgaaaca cgtttcgtca cttcaccttg
    11581 ctcatccatt aatgtctgag tgaaatcttt taccggacca cgcagcggat caaaatcgaa
    11641 ttcattagag aaactggcca tctcaggggt aaatgaaagt gttacctctg tgcggtcaca
    11701 tcctgtgagg aatatcgcga gtaagcatgg cagtaatttg tatttcacaa cagtcaccag
    11761 agagtagaga tgattctcaa tcatagtagc aaatacagta ctttacacgt taaatgctat
    11821 gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa actctcaacc tgctgtgccg
    11881 cgcttctgct cgccctcgcc tcacccgcgg tactcgccgc gccgggatcc tgtgagcgca
    11941 tacagagcga tatatcacag cgcattatca ataatggcgt tcccgaaagc agcttcacgt
    12001 taagtattgt acccaatgac caggttgatc agcctgattc ccaggtcgtc gggcattgcg
    12061 ctaatgatac gcataaaatt ctctacaccc gcaccaccag cggtaacgtc tctgctcccg
    12121 cgcagtctac ccaggatggc gcgcctgccg aaccgcagta atacattatc gactgcacgc
    12181 cggatacgac acatccggcg atttgaacat acaacataat cccaccttat tactcatacc
    12241 cttctattga tatggattaa taattcttaa cccaaaatag gtagactccc tctattgtta
    12301 gcgcgctaaa tattcaatat ataaactttt atataacgat aaagaacagg gagtgagtta
    12361 tgtccaaaaa tgaacaaatg gtaggcatca gccgcagaac actcgttaaa tctaccgcga
    12421 taggttctct ggcgctggct gcaggcggtt tttctttacc gttcaccctg cgcagtgcag
    12481 cggcagcggt acaacaggcc agcgagaaag tggtctgggg tgcctgttcc gtcaactgtg
    12541 gtagccgctg tgcacttcgt ctgcatgtta aagataatga agtgacctgg gtggaaactg
    12601 acaacaccgg caacgacgag tatggcaatc atcaagtacg tgcctgtttg cgcggtcgct
    12661 ccatccgtcg gcgtattaat catcccgatc gcttgaatta cccaatgaaa cgcgtgggca
    12721 aacgcggcga aggcaaattc gaacggatta gctgggatga agccctggat actatcgcca
    12781 gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc tgtatatatt cagtactctt
    12841 cggggattgt tggcggcaat atgacccgct cttcaccatc agcctcggcg gtcaaacgcc
    12901 tgatgaactg ctacggcggt tcactcaacc agtatggctc ctacagcacc gcgcaaattt
    12961 cctgtgccat gccctacacc tacggcagta atgatggcaa cagcaccacg gatattgaaa
    13021 acagcaagct ggtcgtgatg tttggtaaca acccggcaga aacccgcatg agcggcggtg
    13081 gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa cgcaaaaatg attgttatcg
    13141 atccgcgata taccgatacg gctgctggtc gtgaagacga atggctacct attcgcccgg
    13201 gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt gattaacgaa aatctcgttg
    13261 atcaaccttt tctcgataaa tactgcgtcg gctatgacga aaaaacctta cccgcagatg
    13321 caccaaaaaa tggtcactat aaagcctata ttcttggtga aggagacgac aaaacagcga
    13381 aaacgccgca gtgggcttcg caaattaccg gtatcccggt ggaccgtatc atcaagctgg
    13441 cgcgtgaaat tggcacagca aaacccgctt atatctgcca gggctggggg ccgcaacgcc
    13501 aggcaaacgg tgagctgacc gcacgcgcta ttgctatgtt gcctattttg acgggcaatg
    13561 tcggcatcag cggcgggaat agtggcgcgc gtgaatcgac ctataccatt accatagaac
    13621 gcctaccggt gctggataat ccggtcaaaa cgtcaatctc ctgcttcagc tggacggatg
    13681 ctatcgatca tggcccgcaa atgacggcaa tccgcgacgg cgtccggggc aaagataaac
    13741 tggatgtgcc cattaagttc atctggaact acgcgggaaa taccctggtt aatcagcatt
    13801 ctgacatcaa caaaacgcat gaaattctgc aggacgaatc gaaatgcgaa atgattgtgg
    13861 tcatcgaaaa ctttatgacc tcatcggcaa aatatgccga cattctgctg ccagacctga
    13921 tgaccgttga gcaggaagat attattccta acgactacgc cggtaacatg ggatatctca
    13981 ttttcctcca gccagtcacc agcgaaaaat tcgaacgcaa accgatttac tggatcctga
    14041 gtgaggtcgc gaaacgtctt ggacctgacg tctatcaaaa gttcacagaa ggtcgcacgc
    14101 aggaacaatg gttacaacat ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt
    14161 cttacgacga actgaaaaaa atgggtatct ataagcgtaa agatcccaat ggtcattttg
    14221 tcgcctacaa agcatttcgt gacgaccccg aggcaaatcc acttaaaacg ccttccggta
    14281 agattgaaat ttattccagc aagctggcgg aaattgcccg tacctgggaa ctggaaaaag
    14341 atgaagtgat aagcccactg ccagtttatg cctcaacctt tgaaggctgg gactcccctg
    14401 aacgtagaac cttcccactg caactgtttg gtttccatta caaatcccgt acccactcga
    14461 cctacggcaa tattgatgtc ctgaaggcag cctgccgtca ggaggtgtgg atcaacccta
    14521 tagatgcaca gaaacgtggg attgccaacg gcgatatggt gcgggtgttt aaccatcgtg
    14581 gcgaagttcg gctaccagcc aaagtgacac cacgcattct tcctggagtt agcgctatgg
    14641 gtcagggagc ctggcacgag gccaatatgt ctggcgataa aatcgaccac ggcggctgtg
    14701 tgaatacgct aaccactctg cgcccttcac cactggcgaa gggaaacccg cagcacacta
    14761 atctggtcga gatcgaaaaa atataaccca ctacgaccat aaattttgac atgacatttt
    14821 gtttgaaaag caataagtga gtaatgatga aaatccatac cacagaggcg ctcatgaagg
    14881 ctgaaatcag ccgtagaagt ctgatgaaaa cctccgcact tggcagtctt gcgctggcaa
    14941 gcagtgcttt cactctgcca ttttcccaaa tggtccgagc ggcagagaca ccggtagaag
    15001 agaaagcggt ctggagttcc tgcaccgtta actgcgggag ccgctgtctg ttacgtttgc
    15061 atgtgaaaga tgacaccgtg tactgggtgg agtctgatac gacaggtgac gacgtctacg
    15121 gtaatcatca ggttcgggca tgtttacgcg ggcgctctat ccgccgacgg atgaatcatc
    15181 ctgacaggtt gaaatatccc atgaagcgcg tcggcaagcg cggtgaaggt aaatttgaac
    15241 ggataagttg ggatgaagcc ctggatacca tcagtgataa tcttcggcgg atccttaaag
    15301 attacggcaa tgaggctgta catgtcctgt acggaacagg cgtagatggc ggaaacatca
    15361 ccaactcgaa cgtcccgtac cgtctgatga actcttgcgg tggttttctc agtcgctatg
    15421 gcagctacag taccgctcaa atcagtgccg caatgagtta tatgttcggt gccaatgatg
    15481 gcaacagccc ggatgatatc gccaatacga aactggtcgt tatgttcgga aataacccgg
    15541 cagaaacccg gatgagcggc ggtggtgtca cttactacgt cgagcaagcc cgcgaacgtt
    15601 caaatgcacg catgatcgtc atcgatccac gttataacga cacagctgcc ggacgtgaag
    15661 atgaatggct gcccattcgc cctggcaccg atggagcact ggcctgtgcg attgcctggg
    15721 tactgattac tgaaaacatg gtcgatcagc catttctcga caaatattgt gttggttacg
    15781 atgaaaaaac gctgcccgcc aacgcaccac gtaacgcgca ttataaagcc tatattctgg
    15841 gcgaagggcc tgacggcata gctaaaacgc cggaatgggc agcaaaaatc accagcatac
    15901 cggcagaaaa aattatccat ttggcacgag agatcggttc agcaaaacct gcttatattt
    15961 gtcagggttg ggggccacaa cgacactcca acggcgaaca aacgtcccgc gctattgcca
    16021 tgctttccgt tctcaccggt aacgtcggca taaacggcgg caactcaggt gtacgcgaag
    16081 gtagctggga tctgggggta gaatggttcc cgatgcttga gaatcctgtt aaaacgcaga
    16141 tttccgtctt tacatggaca gatgccatcg accacggtac ggaaatgacc gcgacccgtg
    16201 atggtgttcg tggaaaagaa aaactggatg tccccatcaa gtttttatgg tgctacgcca
    16261 gtaacacatt gatcaatcaa catggcgaca tcaatcacac ccatgaggtg cttcaggatg
    16321 acagcaagtg cgagatgatt gttggcattg accacttcat gacggcctcg gctaagtatt
    16381 gcgatatcct gttgcccgac ctgatgccaa cagagcaaga agaccttatc tctcatgaat
    16441 ctgcagggaa tatgggctat gtgatcctcg cccaacccgc aacctcagca aaatttgaac
    16501 gcaaacccat ctactggatg ctgagtgaag tcgccaaacg cttaggaccg gacgtttatc
    16561 aaacctttac tgaaggtcgc agtcagcatg aatggatcaa atatctccat gcgaaaacga
    16621 aggaacgtaa ccctgagatg cccgactacg aggagatgaa aacgaccggg atctttaaga
    16681 aaaaatgccc ggaagaacac tacgtcgctt tccgcgcatt ccgtgaagat ccacaggcaa
    16741 acccgttgaa aacaccttcg gggaaaatcg aaatttattc tgaacgactg gcgacgattg
    16801 cggatacctg ggaattgaaa aaagatgaaa ttattcatcc ccttcctgcg tataccccag
    16861 gttttgatgg ctgggacgat cccctgcgga aaacctatcc actgcagcta acgggcttcc
    16921 actacaaagc gcgtacccac tccagctacg gcaatattga tgtgttacag caggcctgcc
    16981 cacaagaggt gtggatcaac cccattgatg ctcaggcacg cggtatccgt catggcgata
    17041 ccgtgcgggt atttaacaat aatggagaaa tgctgattgc cgcaaaagtg actccgcgta
    17101 ttctgcctgg cgttaccgcc atcgggcaag gtgcgtggct taaagcggat atgtttggtg
    17161 accgggtcga tcacggcggc agtatcaata ttctgacctc tcaccgccct tcaccgctgg
    17221 caaagggaaa cccgtcgcac agcaatcttg tccagatcga aaaggtttaa ggagtagccc
    17281 atgaccacac aatatggatt ttttatcgat tccagccgct gtactggctg taaaacctgc
    17341 gaactggcgt gcaaagattt taaagatctt ggcccggaag tcagtttccg ccgtatttat
    17401 gaatatgctg gcggcgactg gcaggaagat aatggcgtct ggcaccagaa cgtgttcgct
    17461 tattatctct ccatctcctg taaccattgc gatgaccccg catgtacaaa agtctgcccc
    17521 agcggcgcaa tgcataagcg tgaagatggc tttgtggtgg ttgatgaaga tgtttgtatc
    17581 ggctgccgct actgccacat ggcttgtccg tacggcgcgc cacagtacaa tgctgaaaaa
    17641 gggcacatga cgaagtgcga tggttgttat tcgcgcgtcg ccgaggggaa acaacccata
    17701 tgtgtcgaat cctgcccgct gcgtgcactt gagttcggtc caattgaaga gctgcgccag
    17761 aagcacggca ctctggctgc cgtcgcgcca ctgccgcgcg cgcatttcac aaaacccaat
    17821 atcgttatca aacctaacgc caacagccgc ccgaccggcg ataccactgg ttatctggct
    17881 aatccggagg aagtgtaaaa tgggaaatgg atggcatgaa tggccactgg ttatctttac
    17941 cgtattgggt caatgcgtag taggcgcgct gattgttagc ggaattggct ggttcgccgc
    18001 caaaaatgac gcagaccgtc agcacattgt tcgtggcatg tttttcctct ggctgttgat
    18061 gggcatcggg tttattgcct ctgtcatgca cctgggatcg ccactgcggg cgtttaactc
    18121 gctgaatcga atcggagctt ctggtctgag taacgagatt gctgccggtt caatattttt
    18181 tgccgttggc ggattatggt ggctggtgac gatcatcggt aaaatgccgc aagcgttggg
    18241 gaaactctgg ttacttgtca gcatggcgct cggcgtcatt ttcgtctgga tgatgaccgg
    18301 cgtgtatcaa atcgacactg tgccaacctg gcataacggt tacactacgc tggctttctt
    18361 tctgacagta ttattgagcg gtccaatact tgctgctgca attctgcggg cagcccgcgt
    18421 tacatttaat accacaccat ttgccatcat tagcgttctg gcgctgatcg cttgtgccgg
    18481 ggtgattgtg ctgcaaggtt tgtctctggc gtctattcac tcatccgtac aacaagccag
    18541 cgcactggta ccagattacg cctccttaca ggtctggcgt gtggtattgt tatgcacagg
    18601 tctaggatgc tggctttgtc cgctcattcg tcgccgtgaa cctcacgttg ccggacttgt
    18661 tctggggcta atcctcatac ttggcggtga gatgatcggg cgtgtgctct tttatggcct
    18721 gcacatgacg gtaggaatgg ctattgcagg ttaacaaatt ggtgcggggc aacccgcaca
    18781 tttcaggatg ttaggaatga cccatttttc acagcaagat aatttttctg tcgcggcgcg
    18841 cgtgttgggc gcgctgtttt attacgctcc agagagtgcg gaagccgccc ccctggttgc
    18901 ggtactcacc cgtgacggct gggaaactca gtggcctcta ccagaagcgt cattagcgcc
    18961 gctggtgacg gcttttcaga cacagagtga agagactcac gcccaggcct ggcagcgttt
    19021 gtttgtcggc ccgtgggcac tgccgtcacc gccgtggggg tcggtctggc tggatcgcga
    19081 atctgtgctg tttggcgatt caacattggc acttcgtcag tggatgcgcg agaaaggcat
    19141 tcagtttgaa atgcagcaaa acgaaccgga agatcatttt ggttcactgc tgttgatggc
    19201 tgcgtggttg gcggagaatg gtcgccagac agaatgcgaa gaattgctgg catggcacct
    19261 ttttccgtgg tcaacacgat ttcttgacgt ttttatcgaa aaagcagaac accctttcta
    19321 ccgtgcactg ggtgaactgg ctcgcttaac gctggcgcaa tggcaatcac aactgttaat
    19381 tcctgtcgcg gttaaatcgc tgtttcgata ggatcaccgt aatattgccg gatggtgacg
    19441 attacgcgca attcggcaat aatacgttac agtgtcacgt tttttatctc ttaaagcacg
    19501 cactgctttt gcgtctggct tcttttgccg caaaatagtc gcccgtgttt cattgcccat
    19561 ttctgctcat gcatcatcta cacatctatc cggatctgcg cactatgttc cgccgtctgc
    19621 ttatcgcaac agtcgtcggt attctcgcgg cctttgccgt tgccgggttt cgtcatgcaa
    19681 tgctgctact ggagtggttg ttcctcaata atgactcggg cagtctggtc aatgcagcga
    19741 caaatctttc cccgtggcga cggctgctaa ctccggcgct cggcggactg gcggcgggtt
    19801 tgttgctgat gggctggcag aaatttaccc agcaacgccc tcatgcgccg accgattaca
    19861 tggaagcgtt gcaaaccgat gggcagtttg attacgcagc aagcctggtt aaatcgcttg
    19921 cctctctgct ggtagtaacc agcggcagtg caattggtcg cgaaggtgcg atgattcttt
    19981 tagctgccct tgccgcctcc tgttttgtcc aacgttttac gccacgccag gagtggaaat
    20041 tatggatcgc ctgtggagct gcggcgggaa tggctgcggc ctatcgcgcc ccgcttgctg
    20101 gcagtttatt tatagccgaa gtgctgtttg gcactatgat gttggcctct ctcggcccgg
    20161 tgattatttc cgccgtcgtg gcgttgctgg ttagcaatct gattaatcat agcgacgcgt
    20221 tactctacag cgtacaactc tcagtgacgg ttcaggctcg tgactatgcg ctgattatca
    20281 gtacaggtgt gctggcaggt ctgtgcggac cactgttgtt aacgttaatg aacgcctgtc
    20341 atcgtggatt tgtaagtctc aaacttgcgc cgccctggca actggcactg ggcgggttga
    20401 tcgtgggtct gctttccctg ttcacacctg cagtgtgggg caacggctat agcaccgtac
    20461 aatccttttt aaccgccccg ccactgttaa tgatcattgc cgggatcttc ctctgtaaac
    20521 tgtttgccgt gctggcgagt agtggttctg gcgcgcccgg tggtgtcttt acaccgacgc
    20581 tatttatcgg tcttgccatt ggcatgttgt atggtcgtag cctgggatta tggttccctg
    20641 atggcgaaga aattacgctt ttactcggat tgaccgggat ggcgacactg ttggcggcaa
    20701 ccacgcacgc gccgattatg tcgacgttga tgatatgtga aatgaccggg gagtatcagc
    20761 tactccccgg tttattgatt gcctgcgtaa ttgcgtcggt aatttcgcgg actttacacc
    20821 gtgactctat ctaccgccag cacactgcgc agcatagcta agcggatgta ttgccccagt
    20881 tcacgctgtt cggcgcgcgg cagataaggc agttcaccaa tgagcggtgc cggaagtttt
    20941 ttacccagca catcaatgat ttccgcataa tgcgccagtc ctggattgat tcggttagcc
    21001 acccagccaa tgagcggtag cccgtcgttg gcgatcgcct gagctgttag cagtgcatgg
    21061 ttaatgcaac cttcctgaat accgacaacc atcaacaccg gcagttgttc ctgcaccacc
    21121 cattcagaga gcggacgcaa atcattcatc agactgcgcc agccgccagt cccttctacc
    21181 accacatgat cgactttatc ggtcaggttt gccaggccgt ttgaaatgag ggtgtaattg
    21241 attgggcaac tgtgcgccac gctactttct tcttcgctta acgcgatagg attaactgct
    21301 tcataaggca gttcgatggt tgaaacactc tgcaacacca gggcatcttt attacgcagc
    21361 ccttcgggtg tctctttgct ccccttcgct acgggtttat atcccgcaac cgtttttccc
    21421 tgggaggcta acgcttgtag caatgcgcgg gaaaccaccg ttttccctac agaagtgtct
    21481 gtaccggtaa taaagaaacg cttcagcatc actaactcca ccgttatgct tcacaaatat
    21541 aaaccaggaa aataattaac cttgaaagtc taagttatgc tttcctggcc caaattgaga
    21601 tagcgcaaat tttggtagaa cagttaaaaa atgttaaccc tgcaacagac gaatcaacaa
    21661 agaaccgtta tacatcgcgt cttttaccag cgcagcccct gccatcgtgc cctggttaga
    21721 aaactgagta ctctcaacgc taatgtgctg actatacgca ggaagggcct gctgacggat
    21781 gctatctgag atgaccggga agaggatatc tgccgcttta cttaacggtg agccaatcag
    21841 tattttttgt gggttaaata aattcaccat gatggcaaga atgcgcccta catgcgcgcc
    21901 caccccggta atgatgtctt ttgccagcag atcgccgcgc aatgccgcct gacacaatga
    21961 gtccacggtt aacggttgtc catgtaacat cgagctcatg gattgattga gacgcagctg
    22021 tgccagctca agaatactgt ccacactggc gatggtttcg aggcagccgt gattcccgca
    22081 ataacagcgt ttcccatacg ggtcgacctg cgtgtgacct atttccacga ggctactgct
    22141 gcctgcgtgt agcagatgac catcggtaat gacgcccgcc cccacgttgt gatcgataac
    22201 cacctgaatc acatcgcgcg ccccgcgtga ggcaccaaac aaggcctcag ccatcgtcca
    22261 tgcgctgata tcatgctgaa tataaaccgg tacgccggta tgctgctcca gcgcctcgcc
    22321 gagcggcatc tcttttacat cctcgtagaa cggcatgcga tgtacaatac cattttccgt
    22381 atcaataatt cccggcaagg taatggcaat cgaagtcaga cgctcaagtt ttttctggtg
    22441 gcggataaaa aactgatcga tatgggaaat gatacgatcc agcaatggtg agtcatcttt
    22501 taacgccagt tcctgcgcct cctccaccac cagtttgctg ctcagatcgc gcagagcaag
    22561 gaaaatctcc ccgcgactaa tgcgcagaga aagatagtgc caggcttcag tttcaaccac
    22621 cagccccact gccggacggc cacggttccc cgcttctttg atttccagct cttgcaccag
    22681 gtgtgcttcg agcatctcac ggacaatttt agtgatactg gcaggagcca gctgcgccag
    22741 acgggaaaga tcgatacgcg agactggacc aagctgatca atcaggcgat aaaccgcgcc
    22801 cgcgttggtc tgctttattt gatcaatgtg cccaggctga ttttcagcaa ccaccgcata
    22861 ctccctatat tttcgcgctc cgaaataatc tgtaggctat ggtgaagcac ttcaatacgt
    22921 gtcgtcaaat ttttacttag gcatgtgatt aacagcacat ttttcgggct ttttcgctga
    22981 aatttccctc acctgagagc attcagcaga tgaatacgaa agttacgcgc agccggactt
    23041 tgttcatgat gtttcggcca gaccaaccac atttcagaaa ccgcatcctc ttcagcaatc
    23101 ggcacccagc gcatttcgtt gagctgaaca cgtttaaatg acgcaggcaa aattgaaaca
    23161 cccagaccgg cggaaaccag accgatgatg gtcattgcct cacccacctc ctgagtgatg
    23221 acgggcgtca aattgtaacg tctcatcagc ccgagaatat cgtcatacag ccctgtcccg
    23281 acgtgcggat caaaaaagac aaagggttct ttcgccagtt cagccagcgt tacactcggg
    23341 ttatttgcca ggggatgatc gtgcgggatc atcgccataa gcggttcatg gacgatgact
    23401 gcgtgttcaa gcgtctccgg taacgctgtg ttacgcagca atcccatatc cagcgttcct
    23461 tcaatgagcg gagcgatttg ctcgcgagtg ttcatttcgc gggtttgtaa atggacatcg
    23521 ggataatcac ggcgaaacag cgataacgta tcggacaccg cccgaataaa aggagcggac
    23581 gaagtaaaac caatgcgcaa ctcccccgct tcaccctgat gcagtctttc agcgcgagcg
    23641 gcagcatcat ccaccataga caggatttgc cgactatcta caagaaactg ttttcctgct
    23701 gccgtcagca atacactgcg attggtccgt gccagcagtc gggcaccaat ttgttgctcc
    23761 agcgcctgaa tctgttgact tagcggtggt tgcgaaatat tcaggcgggc agcggcgcgc
    23821 ccgaaatgca gctcttctgc aaccgcaaca aagtaacgca gatgacgaag ttcaatattc
    23881 atatttaaaa catcttattt gagattatta atatattaga cagaacaatt cgattttcct
    23941 aatctattca taagcctgat ccacaggcat atttagcaag gatttcaagt gagccgtact
    24001 acaactgttg atggcgctcc ggcaagcgac actgacaagc aaagcatttc tcagccaaat
    24061 caatttatta aacgcggtac gccgcaattt atgcgtgtca ccctggcgct tttctctgcc
    24121 ggactggcaa ccttcgcact tctctattgt gtgcaaccta tccttccggt gctttcgcag
    24181 gagtttggct taacgcctgc taacagtagt atttcactgt ccatttcaac ggcgatgttg
    24241 gctatcggtt tgctgtttac tggcccgcta tccgatgcca ttggtcgcaa accagtgatg
    24301 gtcacggcgc tactgttggc ctccatttgt acgttacttt cgacaatgat gaccagctgg
    24361 cacggcattt tgattatgcg cgccttgatt gggctttcgt taagtggcgt ggcagctgtt
    24421 ggcatgactt atcttagcga ggaaatccac cccagtttcg tggccttttc gatggggttg
    24481 tatatcagcg gcaactcaat tggcggtatg agcggacgct taattagcgg tgtcttcacg
    24541 gactttttca actggcgaat tgctctggcg gcaatcggtt gtttcgcgct ggcctcggcg
    24601 ttaatgttct ggaaaatcct ccctgaatca cgccattttc gcccgacttc gctgcgacct
    24661 aagacgttgt ttatcaactt tcgtctgcac tggcgtgacc ggggattacc gttattgttc
    24721 gcagaaggct ttttgctgat ggggtcgttc gtcacgctgt ttaattacat cggctatcgg
    24781 ttgatgctct ctccctggca tgtcagccag gccgtggttg gcttattatc gctggcttat
    24841 ttgaccggta catggagctc acccaaagcc ggaaccatga ccacccgcta tgggcgcggt
    24901 ccggtgatgt tgttttcgac gggggttatg ctgtttggtt tactgatgac cttatttagc
    24961 tcgctgtggc tgatctttgc cggaatgtta ctcttctcag ccggattctt cgcagcccac
    25021 tcagtagcca gcagctggat cggcccccgc gcaaaacgcg ctaaaggcca ggcctcctcg
    25081 ctgtatctgt tcagttacta tctggggtcg agtattgccg ggacgctggg tggtgttttc
    25141 tggcataact atggctggaa cggcgtcggc gcatttattg ctctgatgct ggtcattgct
    25201 ctgctggtcg ggacgcgttt gcatcgtcgt ctgcacgcct gaaaaataag tccggactgc
    25261 ggtaaatacc catccggact tattgcctgc tcaaaccaac gttaatagcc atcctaaaat
    25321 agacgaagcg ccagccaatt cccgcagcgc atctagtgtc atcaggatta taagtaccca
    25381 aataaacgga ttcattttgc tgtgtgtcat ttattactga tgcgcagtta ttctaccgct
    25441 ttgtaagtag taaaatagtt aacccgatca agactactat tattggtagc taaatttccc
    25501 ttaagtcaca atacgttatt atcaacgctg taatttattc agcgtttgta catatcgtta
    25561 cacgctgaaa ccaaccactc acggaagtct gccattccca gggatatagt tatttcaacg
    25621 gccccgcagt ggggttaaat gaaaaaacaa attgagggta tgacaatgaa aaaagtatta
    25681 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
    25741 accacacctg ctccgactgc gacgaccacc aaagcagcgc ctgcgaaaac cacacatcat
    25801 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
    25861 aatgccaaag ctgaacagaa agctcctgaa caaaaagcgc aggcagcgaa gaaacacgcc
    25921 aagaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttatc
    25981 aatagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc ggataacgtt
    26041 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
    26101 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga tcgtagttat
    26161 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
    26221 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
    26281 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
    26341 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
    26401 cccaatcacc gtgggatgcg gttggccaac tggaaacggc cagcggcaat ttatgtacgg
    26461 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acgccgccaa
    26521 agggtaaagc cgataaagcg gtagcgctgc gtttcgtgtc aaataaaggt ctttggcgct
    26581 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
    26641 atggggatgg ttggattgtt cctcccgcag ctgcgccgtg ggacttcggt ttgattgtgc
    26701 tacgtaatcc gccttctggc attacaccat tgccgttatt tgagggagat aaagccgcgc
    26761 ttaccgccgc attaaaagcg gcaggtcgta aagtgactca ggcgggatac cctgaagatc
    26821 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
    26881 tgatgtcgca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
    26941 ccgatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
    27001 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaggctg gatcaactgt
    27061 cgcaaaaata atgttcaaac gctgcccgac agcgcgggca gcgtgttcat caggcaagtt
    27121 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
    27181 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
    27241 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
    27301 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
    27361 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
    27421 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
    27481 gatttgatca atacaatccc ggccaccagg gtggttaacc cggcaatttt catcagcgat
    27541 aaactttcgt cgaataacaa aacactaaac aaggtaataa ataaaatacc gataccttcc
    27601 cacagcgcat aagccacgcc taaggcgatc tttttaaagg cgaaagagag aaatatatac
    27661 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
    27721 catttcatag acagcgtacc ggtaatttct gtagcaatag ccagacctaa taaaatccaa
    27781 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
    27841 aaccaaagta aagtggtcaa gccccgcgca tagcgtaagc ttaagcagaa gaaaggacta
    27901 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
    27961 ggtaaatgta gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
    28021 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
    28081 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
    28141 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
    28201 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
    28261 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
    28321 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
    28381 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctgt
    28441 tagcttatct gggttccgca ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
    28501 ctattatgac gccgctgcaa gcgattgaac cgttgttgca acgcgtaggg attgatgtct
    28561 cagttgacca actggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
    28621 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
    28681 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
    28741 ttgaagggat ggcggcgatt caacgtgcga ttgacagtgt ttctcattat ctggtgctga
    28801 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
    28861 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
    28921 gttcagttct cgcggcaatt ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
    28981 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
    29041 agccacgcat catggggcgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
    29101 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttgacaatta
    29161 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
    29221 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
    29281 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
    29341 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
    29401 gctttccaca cttccagcac aggcatacca gcaatcggac tcttcggatc atcctgcgcc
    29461 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
    29521 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
    29581 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
    29641 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
    29701 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
    29761 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
    29821 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
    29881 taagaaagga tagcgcccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
    29941 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
    30001 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
    30061 atggaggtca ttatcagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
    30121 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
    30181 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgacccc
    30241 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
    30301 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
    30361 gccagaccca cgaagctatg caggatcgcc accagttctg gcatttcggt catttcaact
    30421 ttcttcgcca gccggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
    30481 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
    30541 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gcccggccag actgaagata
    30601 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
    30661 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
    30721 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
    30781 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
    30841 tgtatgcagc gcgtgcgaaa cattccacac cacgtaataa ccgacaacgc aggccagcgc
    30901 gaacacagtg aagtgcccaa ggaactcttt cggcgcaacg cttgccaacc agccgaaaag
    30961 aatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
    31021 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
    31081 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
    31141 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
    31201 gaggttagta ccgtaaagct gtgaggattg cgtcggcaga cggcccggaa gatcggtgta
    31261 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
    31321 gttaccgccg ttctgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
    31381 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
    31441 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttga tgaacgcgtc
    31501 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
    31561 gaattccgcg cccatacttt gaacttgttc tttcacttcc gggcgggtgt cgaatgcacg
    31621 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
    31681 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
    31741 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
    31801 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagagtcca tcgccatcac
    31861 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
    31921 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
    31981 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
    32041 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
    32101 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
    32161 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
    32221 tgatggtttg cctgtgtcag gagtcacaca agccgctcat gtacgagcta aatgttactc
    32281 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
    32341 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
    32401 tgggataaac accttagaac gccggataaa gactgataat tgtcttcggc ggtcgggtaa
    32461 aacgagacat cgccccggca cgaatcacta cttaacatta aattaactta tacaattcag
    32521 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
    32581 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
    32641 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
    32701 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
    32761 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
    32821 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
    32881 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
    32941 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
    33001 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
    33061 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
    33121 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
    33181 tcgtcaaatc gatgccaacc agggcggcaa ccagcgtatt actgcattca tctataaaaa
    33241 agatgcgaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
    33301 acgtgcagct ctggctgccg gtggcgaagc tgcgaagaaa gttgagatcc cgggtgttgc
    33361 gactaccgca tcaccaagtt ctgaagtcgg tcgcttcttt gaaacccagt catcaaaagg
    33421 cgggcgttac accgtcacgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
    33481 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
    33541 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
    33601 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgt
//
LOCUS       DAOTRK010000041        33485 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.041, whole genome shotgun sequence.
ACCESSION   DAOTRK010000041 DAOTRK010000000
VERSION     DAOTRK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.041"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(176..778)
                     /gene="fimB"
                     /locus_tag="QIE41_001875"
     CDS             complement(176..778)
                     /gene="fimB"
                     /locus_tag="QIE41_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDS3530768.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2231..2947
                     /gene="nanC"
                     /locus_tag="QIE41_001876"
     CDS             2231..2947
                     /gene="nanC"
                     /locus_tag="QIE41_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDS3530769.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2967..4073
                     /gene="nanM"
                     /locus_tag="QIE41_001877"
     CDS             2967..4073
                     /gene="nanM"
                     /locus_tag="QIE41_001877"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDS3530770.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGVIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4138..5118
                     /locus_tag="QIE41_001878"
     CDS             4138..5118
                     /locus_tag="QIE41_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDS3530771.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(5126..5776)
                     /locus_tag="QIE41_001879"
     CDS             complement(5126..5776)
                     /locus_tag="QIE41_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDS3530772.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQEIYVEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            5913..6785
                     /locus_tag="QIE41_001880"
     CDS             5913..6785
                     /locus_tag="QIE41_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDS3530773.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYILAEYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     KIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            complement(6848..8158)
                     /locus_tag="QIE41_001881"
     CDS             complement(6848..8158)
                     /locus_tag="QIE41_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDS3530774.1"
                     /translation="MKSSSHFTVFEYGHLVSEEGDARINNAVVLPADLFKWLELRCLK
                     DDADNTHRLLTLSSRNGVKTLQVKNYAGVISLPGGRFLEILPKTGQDNEDTVRQQLLM
                     MLRTLKSFRHIASSESGVKISKMPLMDIFIQQFIESVRKIVQRGLKRDYLRQEDNLPW
                     IKGRLRISAQLSKNCIRRDLFQVEYDEYSVNRLENRILKTAINKISRQTSNPQLLQQI
                     TQLQFHFENITSVHDAYIAFEQLHFDRHMHHYEQALAWAKMILLGDSPHCMYGDVNAF
                     SLLFPMEAVFESFVTTWMRYRYYDKWRVDAQVSSKNLISYNGKALFKLRPDICLRPRK
                     STTGSVITCDIKWKIVNGRKDSLEQSQADLYQMLAYGLNYQEGEGDMILIYPYHNGFN
                     QPSPHPYEFSHQKENRLRLWVVPFFIGESLQTSELRFPSGAEFI"
     gene            complement(8155..10209)
                     /locus_tag="QIE41_001882"
     CDS             complement(8155..10209)
                     /locus_tag="QIE41_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HDS3530775.1"
                     /translation="MVQDSSALAPFFRAQIEKNNQYNSVWGKHYRELVEQIQAEKPNF
                     SEATIRQVWYERDNGVSSLMQGGMSLVEFENAQKDLLTLTRQIAEECTQENYNRVIER
                     LIKLKADGVLNKVYRALCNRAFAAIYPSKISSVVNVNNFFSCYNYCNNHFQLGLSGER
                     EWLARNVDLKETLNRALGDDIDPIELNMSLWHLYAYEIQDKNAIGLVGSESALQENEQ
                     EDDVISSPLPKNTILYGPPGTGKTYRTIELAVQICDPIAYSLQEGKDESEKRRELKKI
                     YDQLIKDNRVRFITFHQSFGYEEFIEGLRAETTDDGNVKYEIKAGIFKQICEDAAFGH
                     AGAQEMLDSALTRLQERLSEGENITLETQQGKAFQLTYKSQTNFGIFPSQSKKEDLGQ
                     GYNAYLKDIRQVYQDPRAKVHNPSYVRSILNYLINQRYLPLNTQSVASEKKENYVLII
                     DEINRGNISKIFGELITLIEPSKRAGEPESLSVLLPYSSTPFSVPNNLYLIGTMNTAD
                     HSLTALDTALRRRFDFEAMLPDITVLKDTVIKGIDLPRLLQTLNDRIQVLYDREHMLG
                     HAFFIPVVQVKEDEALAFERFKRIMRNKILPLLEEYFYNDWQKIRMVLADNQKEEAPD
                     LQFVREVKYQKKYADLFGDNGTDDLGTSFHLAAEDDNVWDNPIAWQQIYAPQKEKRRS
                     AE"
     gene            complement(10569..11402)
                     /locus_tag="QIE41_001883"
     CDS             complement(10569..11402)
                     /locus_tag="QIE41_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDS3530776.1"
                     /translation="MQTEAEVLTDHNELICSTSIERIVTGRDSALNQIATLIQKLDDI
                     SSLTSSIGGDVAGAWAMRNGYAFDCWLMQPTDKAMPVITRNIDRSIWRDLMLKSGMLT
                     LMDAEARSQWAKNLEEGDLPAISEANILSTFEQLHHNKQDVFERGIINVFKGLSWDYK
                     TNNPCYFGKKIIVNNLVKHDRWGYSLHWGWRGDQLADLERMFYLLDGKAIPDNRHDVA
                     IRFMDFVSAHPHEQVFDDDLFVIRYYQKGSGHITFKRLDLVDKMNDIVAKHYPSALSA
                     K"
     gene            11707..13263
                     /locus_tag="QIE41_001884"
     CDS             11707..13263
                     /locus_tag="QIE41_001884"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDS3530777.1"
                     /translation="MTSLQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEKIMQVFASKEDVAHL
                     AKSVAFEAVVANDYNLSVSSYVEAKDTREIIDIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            13260..14564
                     /locus_tag="QIE41_001885"
     CDS             13260..14564
                     /locus_tag="QIE41_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDS3530778.1"
                     /translation="MSELSYLEKLLDGVEVEWLPLSKVFNLRNGYTPSKTKKEFWANG
                     DIPWFRMDDIRENGRILGNSLQKISSCAVKGGKLFPENSILISTSATIGEHALITVPH
                     LANQRFTCLALKESYADCFDIKFLFYYCFSLAEWCQKNTTMSSFASVDMDGFKKFLIP
                     RPCPNNPEKSLAIQSEIVRILDKFSALTAELTAELTMRKKQYNYYRDQLLSFDEEQEK
                     PIYLEKLLDGVEVEWRTLGEIAVIYDGTHKTPKYTASGVPFVSVQNIKNLYGTNKYIS
                     PEDFKTYKNKPRKHDLFMTRIGDVGTCAIVEDDRPLAYYVTLALIRINQQIAVPGFIR
                     YLIESRFGKAELYKRTLIHAVPMKINLGEIGKVAVPIPCPDNPEKSLAIQSEIVRILD
                     KFDTLTNSITEGLPREIELRQKQYEYYRDLLFSFPKPETVSN"
     gene            14686..17802
                     /locus_tag="QIE41_001886"
     CDS             14686..17802
                     /locus_tag="QIE41_001886"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDS3530779.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKTEQTSDSYQSESDLERELIQDL
                     RNQGYEFIPVKSQSAMLANVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRK
                     IHIDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQFEQAGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDISQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAAN
                     KSATYKPLRVATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSYFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIESEKEKKDFVKLFGEYLRAENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QRREKEADKKEKSTTDWDDVVFEIDLLKSQEINLDYILGLIFEHNRQNKGKGEMTEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDAFFTFAQREQQREAEALIKEE
                     NLNEEAAKRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKIVAFIEK
                     FKGVGGQI"
     gene            complement(18030..18114)
                     /locus_tag="QIE41_001887"
     tRNA            complement(18030..18114)
                     /locus_tag="QIE41_001887"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18078..18080),aa:Leu,seq:caa)
     gene            18310..19329
                     /gene="ahr"
                     /locus_tag="QIE41_001888"
     CDS             18310..19329
                     /gene="ahr"
                     /locus_tag="QIE41_001888"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDS3530780.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            complement(19333..19896)
                     /gene="idnK"
                     /locus_tag="QIE41_001889"
     CDS             complement(19333..19896)
                     /gene="idnK"
                     /locus_tag="QIE41_001889"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDS3530781.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            20113..21144
                     /gene="idnD"
                     /locus_tag="QIE41_001890"
     CDS             20113..21144
                     /gene="idnD"
                     /locus_tag="QIE41_001890"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HDS3530782.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGAEVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            21168..21932
                     /gene="idnO"
                     /locus_tag="QIE41_001891"
     CDS             21168..21932
                     /gene="idnO"
                     /locus_tag="QIE41_001891"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDS3530783.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKNIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            21997..23316
                     /gene="idnT"
                     /locus_tag="QIE41_001892"
     CDS             21997..23316
                     /gene="idnT"
                     /locus_tag="QIE41_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HDS3530784.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEKE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHTDPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            23383..24381
                     /gene="idnR"
                     /locus_tag="QIE41_001893"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             23383..24381
                     /gene="idnR"
                     /locus_tag="QIE41_001893"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HDS3530785.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            24459..25961
                     /locus_tag="QIE41_001894"
     CDS             24459..25961
                     /locus_tag="QIE41_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDS3530786.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEGGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVLAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASTGQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(26080..27162)
                     /gene="lptG"
                     /locus_tag="QIE41_001895"
     CDS             complement(26080..27162)
                     /gene="lptG"
                     /locus_tag="QIE41_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDS3530787.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGLQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDVGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(27162..28262)
                     /gene="lptF"
                     /locus_tag="QIE41_001896"
     CDS             complement(27162..28262)
                     /gene="lptF"
                     /locus_tag="QIE41_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDS3530788.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            28529..30040
                     /gene="pepA"
                     /locus_tag="QIE41_001897"
     CDS             28529..30040
                     /gene="pepA"
                     /locus_tag="QIE41_001897"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDS3530789.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            30174..30617
                     /gene="holC"
                     /locus_tag="QIE41_001898"
     CDS             30174..30617
                     /gene="holC"
                     /locus_tag="QIE41_001898"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDS3530790.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            30617..33472
                     /gene="valS"
                     /locus_tag="QIE41_001899"
     CDS             30617..33472
                     /gene="valS"
                     /locus_tag="QIE41_001899"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDS3530791.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
BASE COUNT         9154 a         7785 c         7971 g         8575 t
ORIGIN      
        1 tttattttga agaacataaa ccattggatt ttgtatgggt atggttaact taatgtttat
       61 tatgataatt aagaatcaca cttacaataa catcaattaa taaatggtta atcatttttg
      121 atatatcgta agaataatgt agtttttaac accatccctg gtatctcaac tatctctata
      181 aaacagcgtg acgctgtcgt cctctggctc tatcccagat gccgtaaaaa cgccctgcat
      241 tgctggcggt ataccagaca gtatgacgaa tattgcgatg cccaagataa tcctggataa
      301 gtcgcgtatc tattcccata ttcgccaaag caaaaccaca cgaatggcgt aacatatgcg
      361 gatgaatctc cagtgacaac ccggcattac caccagaagt cgagataata tggtaaaact
      421 gttgccgaga aagcggattc cccttacgtg ataaaaatac ccactcgctc tcagcatgcg
      481 gataagaagt acggatactc aaccagtttt ttaaagcctg aacttctttg ttcaatagcg
      541 ggtgcgttgt tgaaaagcct ttttttaatc gatggatata tatacacttt gccttaagat
      601 caatatccga aatcctcaat cgacaaattt cactcgcccg gaaaccatga ataaaacaaa
      661 gcaaagtcag acaataatta cgtgctgcat gaggcccggt atttgctgct ttaaggagtg
      721 attcgatttc actatgggtc aggaagttcc tttttttgtt atcagcctta ttcttcatca
      781 tttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
      841 ttaatcctga ttatgtttat aaaagcgaac gtttgcttaa taactaacgt aagtgaacca
      901 gttcttctga gtgaacttaa atggagtagc aacagttaat tataataacg ttgccataca
      961 aacgctatgc tcttgcatgc tatgtacctt tatatattta tcaatcggat acgaatctaa
     1021 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
     1081 acaagtaaaa tcaacataaa aaattcagcg aaatttaata ttcgttcaac tagccgtctc
     1141 acatatgaca ttattagtca actccatttg ccacaaaatg gctatttcat ggaacacagt
     1201 aatttcttag tggctattat caagctaatt cttagatccc tagcgattat tcctggctgg
     1261 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
     1321 gcaggcgacg ctttttcatc tacatccgta ggtaactatg gctttggtca ctacgactta
     1381 aaccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
     1441 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gacctgttac
     1501 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgggta
     1561 aacccttatt ttcaccaagc tcactgagtt agcttgcaaa gctttctttg tttagattct
     1621 aaataaatta tttataaaac agatagttaa aagaaaatcc ttagtttttg aagaccaccg
     1681 caagtgttcg tctggcttca catggcatct gcctcttaga aaaagatcga catattttgt
     1741 gacacgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
     1801 aggtataaag gtgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
     1861 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
     1921 aaatgagatc aacagacact ctattttatg actctgggta aaatgaattg agtaagtgat
     1981 atagcttacg aacattcaaa tcaattaaac atcagaagag attttatact caggtattta
     2041 atctggttct ctgtttattt aaataatgtg gaaagagaat tttcacagga gaccttatac
     2101 aaaaaatata aaataagcta cccgttgcca aagacactat aagcctggca aaaaatatta
     2161 cacaacataa atgctaattg tttatgcggg ctttgtattg ctttctgtat cctacaaatg
     2221 agtgaaattt atgaaaaagg ctaaaatact ttctggcgta ttattactgt gcttttcgtc
     2281 accattaatt tctcaggctg cgacactgga cgtacgtggt ggatatcgta gcggaagcca
     2341 tgcctatgag actcgactca aagtcagtga gggatggcaa aatggatggt gggcaagcat
     2401 ggaaagtaat acctggaata ccattcatga taataaaaag gaaaatgccg cactcaatga
     2461 tgttcaggtt gaagttaatt acgcgattaa acttgatgat caatggacgg tgcgcccggg
     2521 aatgttaacg cattttagca gcaacggcac acgctacgga ccctacgtaa aactgtcctg
     2581 ggacgcgaca aaagatctta attttggcat tcgctatcgt tacgactgga aagcttaccg
     2641 acaacaagac ttatccggtg atatgtctcg tgataacgtt catcgttggg atggatatgt
     2701 cacttaccat attaatagtg atttcacctt cgcatggcaa acgacgctat acagcaaaca
     2761 gaatgattat cgctatgcaa accacaagaa atgggcgacg gaaaatgcct ttgttctaca
     2821 ataccatatg acgcccgata ttacgccata catagaatat gactaccttg accgccaggg
     2881 tgtttacaac ggcagagata atttatcgga aaacagttat cgcattggtg tgtcatttaa
     2941 actgtagtag acaggagaca gtcacaatga ataaaacaat aacggcgctt actatcataa
     3001 tggcttcatt tgccgcaaac gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg
     3061 gtactggagt aattgataac gacactgtct acattggttt aggtagcgca ggtacagcat
     3121 ggtacaagct ggatacacag gccaaagata aaaaatggac agcgttagct gcattccctg
     3181 gtggaccgag agatcaagca acctctgcat ttattgatgg taatctgtat gtgtttggcg
     3241 gcattggcaa aaacagcgag ggcttgactc aggtatttaa tgacgtacac aaatacaacc
     3301 ccaaaaccaa tagctgggtt aaattgatgt cgcacgcgcc gatgggcatg gcgggccatg
     3361 tgacttttgt acacaacggc aaggcttatg ttactggcgg tgttaaccag aatatcttca
     3421 atggctattt tgaagatctc aacgaggctg gaaaagattc aaccgctata gataaaatca
     3481 acgcccacta ttttgacaaa aaagcagaag attatttctt caataagttt ctgttgtctt
     3541 ttgatccctc aacacagcaa tggagttacg ctggcgaatc tccctggtac ggaacggctg
     3601 gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat taatggcgaa gccaaaccag
     3661 gattgcgaac ggatgccgta tttgaacttg acttcaccgg tgataattta aaatggaata
     3721 agcttgctcc cgtcgcatca ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg
     3781 attctcttat atttgccgga ggggccggat tcaaaggttc gcgggaaaat taccaaaacg
     3841 gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag cactgatatt catctttggc
     3901 ataacgggaa atgggataaa tctggtgaat tatcgcaagg tcgggcctac ggagtatcat
     3961 tgccctggaa taatagttta ttaattattg gcggtgaaac tgcaggcggc aaagcggtga
     4021 cggattcagt tttaatttct gtgaaggata ataaagtcac agtacaaaac taacgcttca
     4081 gggccccggt aaaggggccc tgctatcaat ttgtcatttg tattacgcga aaattatatg
     4141 aacgcaataa tatcgcccga ttattactat gttcttaccg ttgctggtca gtctaatgcc
     4201 atggcgtatg gcgaaggact gccattaccg gacagggaag atgcgcctca tcccagaatt
     4261 aaacaattag cgagatttgc gcatacgcat cccggaggcc cgtcatgtca ctttaacgac
     4321 attattccac tgactcactg cccacacgat gttcaggata tgcagggtta tcaccatcct
     4381 ctggcaacga atcatcaaac acagtacggc accgttggcc aggcactgca tatagcacgg
     4441 aaattactgc cctttattcc tgataatgca ggggttctca tcgttccgtg ttgccgtggc
     4501 ggatcggctt ttaccgcggg cagcgaagga acatattcag aacggcacgg agccagccat
     4561 gatgcttgtc gttggggatc ggatactccg ctataccagg atttagtcag cagaacgcga
     4621 gccgcactgg caaaaaatcc gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa
     4681 tttgacttaa tgaccagtga ctacgcgtca caccctcaac actttaatca tatggttgaa
     4741 gcctttcgta gggatctaaa acaataccat tctcagctta ataatattac tgacgcaccg
     4801 tggttttgcg gcgataccac ctggtactgg aaagaaaatt tccctcatgc gtatgaagcg
     4861 atttatggca attatcaaaa taatgtttta gccaatatta ttttcgtcga cttccagcaa
     4921 caaggtgaaa gaggactgac gaacgcgcct gatgaagatc cggacgattt aagcacggga
     4981 tattacggtt cagcgtaccg gtcaccggag aactggacga cggcactgcg aagcagtcat
     5041 ttcagcgcgg cagcccgtcg ggggattatt tctgacaggt ttgtagaagc aattttgcag
     5101 ttttggcgcg aaaggtgagc gttcattaat ttgatttatt cgcatccatc aacgctttta
     5161 actcctccgg cgtaaacggt ggtttgcgtc ggtgcagcat cgcatggcaa ttagggcaca
     5221 caggaagcaa atcattaact ggatccagct ggtaatcctc tttaatggct gaaagcggca
     5281 ccaggtgatg cacatgaata aagccttcag cgatatcacc atagatcttc accagatcaa
     5341 cgccgcatac gttgcactgg catccatgat gttcgaccgc tttatctcgg gcctttttat
     5401 cgcgttcata gcggtttact gtcaccttta tcgccgcacc ttcaacgtat atctcctgcg
     5461 acggcaactc atccgggaac agaacgtcgg ctatgggagc cgcagcggct ttatcctgat
     5521 gaatatgaaa tccctgctct atgagtagct tgacacattt agattttatt ccgccggtga
     5581 aatccgcagg ggtaaattgc gtccccgtca tcaatgtcgc tgcgatgcca gcaatcgcct
     5641 tcgaggggta acgtcggcca tgatattcaa tctcatacaa ccgcgcaggc tggaactgat
     5701 gagtttctcc cgcatcccat aaatggatag cttcgatgat ataccgccgc ggaagatgat
     5761 cagggagttt attcatggag aatgtcctta tacgttctca gtacaggcag caaaaaaaca
     5821 aatgagatca aggtaatata tcttcacttg caggcttgaa cagcagaaaa tttgcgagca
     5881 tgatcgcaat tatttcactg ccagggatat caatggcttc cagtaaatcg ctacagcagg
     5941 caattgccaa tataaaaatc tggcacaagg gtgaacagcg cgcgccgcat aagccattgt
     6001 tattgctata tatattagcg gaatacctga atggacatcc gcgccttttc gattatggct
     6061 cggaaatcta cgaacctctg cacagtttac tggaacgttt tgggccacag cgttcacagt
     6121 accgacctga tatgccgttc tggcgattac aaggcgacgg attctggcaa ttacacaatg
     6181 ccgagctttg ctcaacagca gggagcagcc gacaaccgcc agtgaaagaa ctgaacgaat
     6241 accacgttgc aggcggtttc gatgaacagc actacgccct ggtaaccggc aataaaaaac
     6301 tcatcaatac tctggcgcaa cagatactcg aagcacactt caccgagagt attcaggaag
     6361 aaattgctga cgagctgggt tttgatctcc agaaaatccg taaacagcgc gacccgttat
     6421 ttcgcaaaaa tgtattacgc gcttataact accagtgtgc gatatgtggc tttaatatgc
     6481 gccacgacga caccaccgtc gcgctggaag cggcgcatat aaaatggaaa cagcacggcg
     6541 gtccttgtga aattcctaat ggcctggcgc tttgcgcaat tcaccataaa gcctttgata
     6601 aaggatcgat tggcctggat gaaaatatgc gggtactggt ctcggatgcg gtaaacggcg
     6661 gagggatcgt tgagagatta ttctgggatt ttgacgggaa aactattgcg ttgccgcagg
     6721 tgcgtaaaaa ttacccttat gaggtgtttg tagagtggca tcggaaagag gtttttaggg
     6781 ggtgaggtag gctatcttgt gcatacaaat agacaaataa attatataaa gagatagcct
     6841 acaaatacta aataaattca gccccactag gaaaacgcaa ctctgatgtc tgtaaactct
     6901 cgccaataaa gaaaggtaca acccataaac gtaacctatt ttccttctga tggctaaatt
     6961 cataaggatg aggagatggc tgattaaatc cgttatgata ggggtatatc aaaatcatat
     7021 caccttcccc ctcctggtaa ttcaatccat aggcaagcat ttggtagaga tcggcttgag
     7081 attgttcaag actgtccttt ctgccattta ctatcttcca tttaatatca caggtaataa
     7141 ctgaaccagt agtactttta cgaggccgta aacaaatatc aggccgcagc ttaaataaag
     7201 ctttaccgtt ataactaatc agattttttg aacttacctg tgcgtccact cgccatttat
     7261 cataataacg ataacgcatc catgtcgtca caaaagattc aaaaacagcc tccatcggga
     7321 aaagcagtga aaaagcatta acatccccat acatacagtg tggagaatcg cccaataaaa
     7381 tcatcttcgc ccacgctaat gcttgctcat agtgatgcat gtgtctatca aagtgtaact
     7441 gctcgaaagc tatataagca tcatgaactg acgtgatatt ttcaaagtga aactgcagct
     7501 gtgttatctg ctgtaataat tgtggattac tggtctggcg actaatctta ttgattgcag
     7561 ttttgagtat ccggttttcc agacgattga cgctgtactc gtcatactcc acctgaaaca
     7621 gatcccgcct aatacagttt ttgcttaatt gtgcagagat ccgtaatctt ccttttatcc
     7681 atggaagatt atcttcctga cgtaaatagt cacgttttaa ccctctctgc acaatcttac
     7741 gcacgctctc tataaattgt tgaataaaga tatccatcag aggcatttta gatattttga
     7801 cgccagattc agaggaggca atatgacgaa atgattttaa cgtacgtaac atcatcagta
     7861 attgttgacg aacagtatcc tcattatcct gacctgtttt aggcaggatt tcgagaaatc
     7921 gcccccctgg cagtgagatt actcctgcat agttttttac ctgcagcgtt tttactccat
     7981 tccggctgct aagggtgagc aaacgatgag tattatcagc atcgtctttc agacagcgca
     8041 gctcaagcca tttaaatagg tctgcaggta gcactaccgc attatttata cgggcatcac
     8101 cttcctcact cactaaatgc ccgtattcga acacagtgaa atgactggaa gatttcattc
     8161 cgcactcctg cgtttttctt tttgaggtgc ataaatttgc tgccaggcga ttggattgtc
     8221 ccaaacgttg tcatcctctg ctgccaggtg aaaactggtt cccaagtcat ctgttccgtt
     8281 atcaccaaag agatcggcat actttttctg gtatttcacc tcacgtacaa attgaagatc
     8341 tggtgcttcc tctttttggt tatcagccag taccatgcga attttttgcc agtcattgta
     8401 aaaatattct tccagcaatg gtagtatctt atttcgcata atccgtttga atctttcaaa
     8461 tgccagagct tcgtcttctt ttacctgcac aacagggata aaaaaagcat gccccagcat
     8521 atgttctcta tcgtataaaa cctggatgcg atcgttaagc gtctgcaata gacgaggcag
     8581 atcaatacct ttaatgacag tatctttcag aacagtaata tctggcagca tcgcttcaaa
     8641 atcaaatctg cgacgtagtg cagtatcaag tgcagtaagc gaatgatcag cagtgttcat
     8701 ggtaccaatt aaataaaggt tattcggtac actaaatgga gtggaagagt atggcagcaa
     8761 tacgctaagc gattctggct caccagcccg tttagatggc tcaataaggg taattagttc
     8821 accaaaaatt tttgaaatat taccccgatt tatttcatca ataatgagaa catagttttc
     8881 ctttttttct gaagctacgc tctgagtatt cagaggcaga taacgttgat taattaaata
     8941 gttaaggata ctgcgtacat aagatggatt atgtactttt gctcgtggat cctgataaac
     9001 ttgccgaata tccttcaaat aggcattgta accctgtcct aaatcctctt ttttcgattg
     9061 tgacgggaaa attccaaagt tggtttgact tttatacgtc agctggaagg ccttaccctg
     9121 ttgtgtttcc agcgtgatat tttcaccttc agataaacgt tcttgtaggc gagtaagtgc
     9181 gctatccaac atttcctgcg caccagcatg accaaaggcg gcatcctcgc atatctgttt
     9241 aaaaatacct gcctttattt cgtattttac attaccgtca tcagtagtct cagctcttaa
     9301 accttcaata aactcttcat aaccaaagct ctgatgaaag gtgataaaac gcactcgatt
     9361 atctttgata agctgatcat aaatcttttt caactcgcga cgtttttcac tctcatcctt
     9421 accctcctgc agggagtaag cgatcggatc acaaatttgt acagcaagct caatagtacg
     9481 atatgtttta cctgtacctg gcgggccata gagaattgtg tttttaggca atggcgaact
     9541 tataacatca tcttcttgct cattttcttg taaagcactt tcgcttccaa ctaaaccgat
     9601 agcattttta tcctgaattt catacgcata gagatgccaa agtgacatat tcagctcaat
     9661 agggtcgata tcatcaccca gcgcacgatt tagcgtttct ttaagatcaa cattgcgagc
     9721 cagccactcc cgttctccgg aaagacctag ctggaaatgg ttgttgcaat aattgtagca
     9781 actaaaaaaa ttattaacat tcacgacact gctaattttt gatgggtata ttgctgcaaa
     9841 agcacgatta caaagagccc tatatacctt atttaatact ccgtccgctt taagtttgat
     9901 aaggcgttca atgacccgat tataattttc ttgtgtacac tcttctgcta tctgacgggt
     9961 gagcgttaga aggtcttttt gtgcattttc aaattcaacc aatgacatcc caccttgcat
    10021 caggctggag acaccattgt cacgttcata ccatacctgc cgaatcgtgg cttcagaaaa
    10081 gtttggtttt tcagcctgaa tctgttctac aagctcgcgg tagtgtttgc cccaaactga
    10141 attgtattga ttattttttt caatttgagc gcgaaagaag ggggctaatg ctgatgaatc
    10201 ctgaaccatc tcttaatcca atatttaggg gggagggaaa ttgggatagc cttatcttat
    10261 cggattaatc tcaaaaatgt taaattttat gataaaaaaa taactttcat cctgaaatta
    10321 ccttctgccc tggagccgcc acatttatct gatgactttg aactgcaaaa cgcatggtgt
    10381 tggtggttct cagagaaggc catagcattc tttcagacgt aaaatcgcta ctcgtttttc
    10441 aacctgtgtg cattttctag ttgcataggg aatcaactgt ttttcaggta ggtattgttt
    10501 acagtaaatg gatatgccga aattcagagg gttccagtaa cgacattttg acagcaaaca
    10561 tacgacattc attttgctga taacgctgac ggatagtgct tcgccacaat gtcattcatc
    10621 ttatccacca gatccagtcg cttgaaagtg atatgcccac tgcctttctg gtaatagcgg
    10681 atgacaaata aatcgtcatc aaatacctgt tcatgcgggt gagcactaac gaagtccata
    10741 aaccgaatcg caacatcatg ccggttatcg ggaatggcct tcccatccag cagatagaac
    10801 atccgttcca gatcggcaag ctgatctcct cgccagcccc agtgcaggct gtaaccccat
    10861 cggtcatgct tcaccagatt gttaacgata atcttcttac cgaagtaaca gggattattg
    10921 gttttgtaat cccagctcaa tcccttaaat acattgataa tcccacgttc gaaaacgtcc
    10981 tgcttattgt ggtgaagttg ttcaaaggta ctcaggatgt tggcttcgct aatcgccggg
    11041 agatcgccct cctccagatt ctttgcccac tgactgcggg cttcggcatc catcagagtc
    11101 aacatcccgg acttcagcat gagatcgcgc cagatgctgc gatcgatatt gcgggtgatc
    11161 accggcatcg ctttgtcggt tggttgcatt agccaacagt caaaggcata tccattacgc
    11221 attgcccagg ctccagccac atcaccgcca atactggatg tcaggctgga gatatcatca
    11281 agtttttgga ttaacgtcgc tatctggttc agtgcactat cacgtcctgt gacgatgcgc
    11341 tcaatactgg tcgagcaaat tagctcgtta tgatcggtta agacttcagc ttctgtttgc
    11401 ataatctttc atccgtaaaa aatacactcg ccgttagtag cagacggatt atccactggt
    11461 gaaagattgg gtaaataatg agattgaaaa tcgggaaggt gactcgcaat aaattatctg
    11521 atcacatacg gatggacgat gggcatataa agtagcctcc tgacgcaagc ggcgatgctg
    11581 aataagatgc actaacaatc tggtactgaa tcccctcctc accgtcatac aaatctgaaa
    11641 aaccaaaaaa cttccggtaa aatcccggca aaagccgacg ttaaaaacac attcaaagga
    11701 taaaaaatga ccagtcttca gcagcgcgcg gagcttcatc gccaaatctg gcaaattgct
    11761 aacgatgtca gaggttcggt cgatggatgg gattttaagc aatacgttct gggcgcgctt
    11821 ttctaccgtt ttatcagcga aaatttttcc agctatattg aagccggtga tgacagcatc
    11881 tgttatgcga aactggatga cagcgtaatt actgatgaca ttaaagacga tgccatcaaa
    11941 accaaaggct acttcatcta ccccagtcag cttttctgca acgtagctgc gaaagcaaat
    12001 accaatgaca gactgaatgc agatttaaac agcatcttcg ttgctatcga aagttctgct
    12061 tacggttacc cttcagaagc tgacatcaaa ggtttgtttg ctgatttcga taccaccagt
    12121 aaccgcctgg gtaacaccgt taaggataaa aatgcccgcc tggctgcggt tctgaaaggg
    12181 gttgaagggt taaagcttgg cgactttaac gaacatcaga ttgacctgtt cggcgatgcc
    12241 tatgagttcc tgatttctaa ctatgcggcg aatgctggta agtcaggcgg cgagttcttt
    12301 acaccgcagc acgtctctaa gctgattgca caactggcaa tgcacggcca gacccacgtt
    12361 aacaaaatct acgacccggc agcaggttcc ggttcgctgt tgttgcaggc taaaaaacag
    12421 tttgatgacc atatcatcga agaaggtttt tttggtcagg aaatcaacca tacgacctat
    12481 aacctggcgc gtatgaacat gtttttgcac aacatcaact acgacaagtt tgatatcaag
    12541 ttgggcaata cgctgactga gccgcacttc agagatgaaa aaccgtttga tgccatcgtt
    12601 tctaacccgc cgtattcggt gaaatggatt ggcagcgatg acccgacgct gattaacgat
    12661 gaacgttttg ccccggctgg cgttctggcc cccaaatcaa aagctgactt cgcctttgta
    12721 ctacatgcgc tgaactatct ttctgccaaa ggtcgtgctg cgattgtttg cttcccgggt
    12781 attttttacc gtggcggcgc ggagcagaaa atccgtcagt atctggtcga taataactat
    12841 gtagaaaccg tgatttcact ggcaccaaac ctgttctttg gaaccaccat tgccgtaaac
    12901 attctggtgc tgtctaaaca taaaacggat accaaagttc agtttattga tgccagcgaa
    12961 ctgttcaaaa aagagactaa caacaacatt ctgaccgatg cccatataga aaaaattatg
    13021 caggtatttg ccagcaagga agatgttgct catctggcga aatctgtcgc gtttgaagcc
    13081 gttgtcgcga atgactataa cctgtcagtg agcagctatg tggaagcgaa agatactcgc
    13141 gaaattatcg atatcgctga gttgaatgca gaactgaaaa ccacggtcag caaaatcgac
    13201 cagttgcgta aagatattga tgcgattgtg gctgaaattg aaggctgcga ggtgcagaaa
    13261 tgagcgagtt gagttatctg gaaaaattgc tggatggggt tgaggttgag tggttgccac
    13321 tctcaaaagt attcaactta aggaatggtt atacaccgtc taaaactaaa aaagaatttt
    13381 gggcgaatgg tgatatacct tggttcagaa tggatgacat acgtgaaaat ggtagaattc
    13441 tagggaattc acttcaaaaa atttcatcct gcgccgtaaa agggggaaaa ctatttcctg
    13501 aaaattcaat attaatatca acgtcggcaa caattggtga gcatgcttta atcactgtac
    13561 ctcatctagc caatcaaagg tttacatgtt tggctttaaa agagtcttat gctgattgct
    13621 ttgatattaa atttttattt tactactgtt tttctttagc tgagtggtgc caaaaaaaca
    13681 caacgatgtc cagttttgcg tctgttgata tggatggatt caaaaaattt ttaatccctc
    13741 gcccctgccc gaataatccg gaaaaatccc ttgccatcca gtctgaaatc gttcggattc
    13801 tggacaaatt cagcgccctt acagcagagc ttacagcaga gcttaccatg cgtaaaaaac
    13861 aatacaacta ctatcgcgac cagttgttga gttttgatga ggagcaggaa aaacctattt
    13921 atctggaaaa attgctggat ggggttgagg ttgagtggag aactttagga gaaatagcag
    13981 taatttatga tggaactcac aagactccca aatatacggc ttccggagtt cctttcgtta
    14041 gcgtgcagaa tatcaaaaat ctatacggga caaataaata tatttcacct gaggatttca
    14101 aaacatacaa gaacaaaccg cgcaagcatg atttgtttat gacgaggatt ggcgatgtcg
    14161 gtacttgtgc tattgtggag gatgatagac cattagcata ctacgtaaca cttgcattaa
    14221 tacgaataaa tcaacaaatt gctgtaccag gattcattcg ttatttaatc gaaagcagat
    14281 ttggtaaggc cgaattgtat aaaagaacgc ttatacacgc agtaccaatg aagattaatt
    14341 taggcgaaat tggtaaagtt gctgtaccaa tcccctgccc ggacaaccca gaaaaatccc
    14401 ttgccatcca gtctgaaatt gttcggattc tggataaatt cgacaccctg accaactcca
    14461 tcaccgaagg tctcccgcgt gaaatcgagt tgcgccagaa acaatacgaa tactaccgtg
    14521 atttactgtt cagtttccca aaacctgaaa ctgtcagtaa ttaattgacc attgctaccg
    14581 accgggccac cttaacaccc ggtcagtata tagactattt tttacacgcc ggaagtcgcc
    14641 cttaaagccc ttccggccct tgccagacgg cacaaaggat gcgctatgac tcatcaaaca
    14701 cataccattg ctgaatccaa taactttatc gtccttgata agtacatcaa aactgagcaa
    14761 acaagcgaca gctatcagag cgaatcggac ctggaacgtg aactaattca ggacctgcgg
    14821 aatcagggct atgagtttat acccgtaaaa tcacagtcag cgatgctggc caacgttcgg
    14881 gaacagcttc agaacctcaa tggtgtggtg tttaatgaca gcgagtggcg gcgtttcacg
    14941 gagcaatatc tggacaaccc gagtgatggc attctggata agacccgtaa aatccatatc
    15001 gactatattt gcgactttat ttttgatgac gagcgtcttg agaacatcta tttgatagat
    15061 aaaaagaatc tcatgcgcaa taaggtgcag attatccagc agtttgaaca ggcgggttct
    15121 catgctaacc gttatgacgt cacgatcctg gttaatggct taccactggt acaaatcgaa
    15181 ctgaaaaagc gcggcgtggc gattcgtgag gctttcaacc agatacatcg ttacagtaaa
    15241 gagagtttta acagcgaaaa ttccctgttt aagtatctgc aactgtttgt catttctaac
    15301 ggcaccgata cccgttactt tgccaacacc acaaagcgcg ataaaaacag ttttgacttc
    15361 accatgaact gggcgaaatc agacaacacg ctgattaaag acctcaaaga ttttaccgct
    15421 acctttttcc agaaacatac tctgcttaat gttctggtga actacagcgt gttcgatatt
    15481 agccagacgc tactggtgat gcgaccgtac cagattgccg ccaccgagcg cattctgtgg
    15541 aaaattaaga gctcctttac agcgaagaac tggtcaaaac cggaaagcgg tgggtatatc
    15601 tggcacacca cgggttccgg taaaaccctg accagcttta aagccgcgcg tctggcaaca
    15661 gaactggact ttattgataa agttttcttt gtggtcgaca ggaaagacct cgattaccag
    15721 accatgaagg aatatcagcg tttttcgcca gacagcgtca acggctcgga aaataccgca
    15781 ggccttaaac gaaatctgga taaggacgat aacaaaatta tcgtcactac tattcagaaa
    15841 ctcaataacc tgatgaaagc agaaagcgac ctgcctgtat ataatcagca agtggtgttt
    15901 atatttgatg aatgccaccg cagccagttt ggagaagctc agaaaaacct gaagaagaaa
    15961 ttcaaacgct attatcagtt tggttttacc ggcacaccta ttttcccgga aaacgcttta
    16021 ggctcagaaa cgaccgccag cgtatttggt cgtgaattgc attcgtatgt gattaccgat
    16081 gcgattcgtg acgaaaaagt gctcaaattc aaggtggact acaacgatgt gcggccacag
    16141 tttaagtctt tagagacaga aaccgacgag aaaaaactga gtgcggctga aaatcagcag
    16201 gcatttcttc atcccatgcg tatacaggaa attacgcaat atattctgaa taacttccgc
    16261 cagaagaccc accgtacttt cccaggttcc aaaggcttta atgccatgct ggcagtgagt
    16321 agcgtggatg ccgcgaaagc ctattacgcg acgtttaaac ggttacaaga ggaagcagct
    16381 aataaatcgg ctacctataa accgctgcgt gttgcaacaa tcttctcctt tgccgccaat
    16441 gaagaacaaa atgccattgg tgaaatttcc gatgaaactt ttgataccag cgcaatggac
    16501 agcagtgcta aagagtttct tgacgctgca attcgtgagt ataacagcta ttttaaaact
    16561 aacttcagca ccgacagtaa cggttttcag aactactatc gtgatttagc ccaacgggtt
    16621 aaaaatcagg atatcgatct gttaattgtc gttgggatgt ttttaaccgg cttcgacgct
    16681 ccaacattga acacgctatt cgtcgataaa aacttgcgtt ttcacggcct gatgcaggca
    16741 ttctcccgca ccaaccgcat ttatgacgct actaaaacct tcggtaacat cgtcactttt
    16801 cgggatctgg aacgctcaac cattgatgcc ataacgctgt ttggtgataa aaatactaaa
    16861 aatgtggtgt tagaaaagag ttatgctgag tatatggaag gctttacaga tgctgccact
    16921 ggtgaagcta aacgcggctt tatggcagta gtttcagaac tggaacaacg gttccctgac
    16981 cctgccagta ttgaaagtga aaaagagaag aaagacttcg taaaactgtt tggtgaatac
    17041 ctgcgtgccg agaacatcct gcaaaactat gatgaatttg ccacactgaa agccctgcaa
    17101 caaatcgatc ttagcgatcc tgttgcggta gaaaaattca aagcagaaca ttatgtggat
    17161 gatgaaaagt tcgctgaatt gcaaatgatt cgtctccctg ctgaacgcaa gattcaggat
    17221 tatcgttctg cctataacga tattcgtgac tggcagcgcc gtgagaaaga agctgacaaa
    17281 aaagaaaaat caaccactga ctgggatgac gtagtttttg aaatcgattt gctgaagtct
    17341 caggaaataa acctggatta tatccttgga ctgattttcg aacacaacag acaaaacaaa
    17401 ggtaagggcg aaatgaccga agaggtcaaa cgcttaattc gttcaagcct tgggaaccgt
    17461 gccaaagaag gtctggtggt cgattttatt caacaaacaa acctggatga tttgccggat
    17521 aaagccagca tcattgacgc gttttttacc tttgctcaac gcgaacagca acgtgaagca
    17581 gaagcattga tcaaagaaga aaatctcaat gaagaggcgg cgaaacgcta tattcgcacg
    17641 tctttaaaac gcgaatacgc caccgaaaat ggcacggaat taaacgagac attgccaaaa
    17701 ctcagtccgt taaacccgca atataaaacg aaaaaacaga cggttttcca gaagatcgtc
    17761 gcgtttattg aaaagtttaa aggtgtaggc gggcagatat aatgtattaa tccgaacctg
    17821 atctggcaga tagctgtcag atcaggactg agctaatcca actaattaat gtcgaaccga
    17881 aaataccaac gtctgtacca acatttttcg tttgcgatgg gttgaaattg gctggctctg
    17941 agagagttaa aatcgcgaga aagataggta actaatggat cactggtaca aaaaaagccg
    18001 ctcttgagcg acttgatttg catacggtgt ggtgccgaag gccggactcg aaccggcacg
    18061 tatttctacg gttgattttg aatcaactgc gtctaccgat ttcgccactt cggcactgaa
    18121 gaggtatgcg gaaaacgttg tggattatac ctgtcgcacg ccaccatgca agcgccagct
    18181 tgctcgcacc tcgctaagtg ctgaaatttt cagcattgca taccgcgatg tgtaaccttt
    18241 gtcacactcc aggcaccccg ccctgccatg ctctacacta cccagaccac accagagaag
    18301 gataaaaaca tgtcgatgat aaaaagttat gccgcaaaag aagcgggcgg cgaactggaa
    18361 gtttatgagt acgatcccgg tgagctgaag ccacaagatg ttgaagtgca ggtggattac
    18421 tgcgggatct gccattccga tctgtcgatg atcgacaacg aatggggatt ttcacagtat
    18481 ccgctggttg ccgggcatga ggtgattggg cgcgtggtgg cactcgggag cgccgcgcag
    18541 gataaaggtt tgcaggtcgg tcagcgtgtc gggattggct ggacggcgcg tagctgtggt
    18601 cactgcgacg cctgtattag cggtaatcag atcaactgcg agcaaggtgc ggtgccgacg
    18661 attatgaatc gcggtggctt tgccgagaag ttgcgtgcgg actggcaatg ggtgattcca
    18721 ctgccagaaa atattgatat cgagtccgcc gggccgctgt tgtgcggcgg tatcacggtc
    18781 tttaaaccac tgttgatgca ccatatcact gctaccagcc gcgttggggt aattggtatt
    18841 ggcgggctgg ggcatatcgc tataaaactt ctgcacgcaa tgggatgcga ggtgacagcc
    18901 tttagttcaa atccggcgaa agagcaggaa gtactggcga tgggtgccga taaagtggtg
    18961 aatagccgcg atccacaggc actgaaaact ctggcggggc agtttgatct cattatcaat
    19021 acagtgaacg tcagcctcga ctggcagcct tattttgagg cgctgacgta tggcggtaat
    19081 ttccacaccg tcggtgcggt tctcacgccg ttgccggttc cggcctttac gctgattgcg
    19141 ggcgatcgca gtgtctctgg ctctgctacc ggtacacctt atgagttgcg taagctgatg
    19201 cgctttgccg cccgcagcaa ggttgcgccg actaccgaac tgttcccgat gtcgaaaatt
    19261 aacgacgcca tccagcatgt gcgcgacggt aaggcgcgtt accgcgtggt gttgaaagcc
    19321 gatttttgaa aatcattcgc agcgctgatc tgaggcgctg ccctctttcg cacatattct
    19381 gttttgtcgt atcgccagca cggcctgccg acattgttcg gtgacattgg caatatcatg
    19441 gttgatatca atgcgcacaa tatcctgttc atctgcttgt ggacgctcca gcgcctcaaa
    19501 ctgacttttt agtaacgcta ccggcataaa atgcccagcc cgccgctgca ttcgcgcgag
    19561 aatagtttca tagtcgccat ctaaccagag gaaatgaaca tgggggctac ccttgcgtaa
    19621 aatatcacga tactgttttt ttaatgatga acagacaata aatcctgttt cattcttttt
    19681 ataaagactg tatgaagcat catttaagcg ttccagccag ggaagtcgat cttcatcaga
    19741 taatggaata ccctgcgaca ttttatctat atttttggct ggatgaagat cgtcaccatc
    19801 aataaattta gcagataata acgcggcaac cttgctacca attaatgttt taccactccc
    19861 tgaaacgccc atcaaaataa agctttcacc cgccatttaa aatctcctga atcatcttac
    19921 ctgctgcgat actacgcctg gtggcataat aatttcacgt ttttttattt ctgctgataa
    19981 gaattacaag gcacatcacg ttatgcgtaa catagtaatg taacaatttt ctgacgtgat
    20041 cttcatcaca aataatgaca attaaaaccg cttaaatgct tccgaggtgt ctacctgacc
    20101 agtgaggaat ttatgcaagt gaaaacacag tcctgcgttg ttgcaggcaa gaaaactgtt
    20161 gccgttaccg agcagacgat agattggaat aataatggaa cattagtaca aataacccga
    20221 ggtggaattt gcggttccga tttacattat tatcaggaag gaaaagtagg taatttcatg
    20281 ataaaggcac cgatggtgtt aggtcatgaa gttatcggta aagttattca tagcgactca
    20341 tcagaattac atgaagggca aacggtagcc attaatccgt ctaaaccgtg cggtcactgc
    20401 aaatactgca ttgaacataa cgagaatcag tgtacagata tgcgtttttt tggcagtgcc
    20461 atgtatttcc ctcatgttga tggtggtttt acccgttata aaatggtcga aacgtcgcaa
    20521 tgtgtccctt atccggccaa agctgacgaa aaggttatgg cttttgccga acctttagcc
    20581 gtcgcgattc atgccgcaca tcaggccggc gagttacagg gcaagcgagt atttatttct
    20641 ggtgttgggc ccattggctg cctgattgtc agtgctgtga aaacactggg ggccgcggaa
    20701 attgtctgtg ccgatgtgag tccccgttcc ctttcgctgg gcaaagagat gggggcggag
    20761 gtgctcgtaa acccacaaaa cgacgacatg gatcactgga aagcggaaaa aggctatttc
    20821 gatgtcagct ttgaagtgtc cggtcatcct tcatcagtga atacctgtct ggaggtcact
    20881 cgtgcacgcg gcgtaatggt gcaggtaggt atgggaggcg caatggcaga attcccaatg
    20941 atgacattga ttggtaagga gatttcactc aaaggctctt tccgttttac cagcgaattt
    21001 aataccgcag tgtcatggct ggcgaatggc gttatcaatc cactgccttt actgagtgct
    21061 gaatatccct tcactgacct ggaagaggcg ctacgtttcg ccggtgataa aacccaggca
    21121 gcaaaagtcc agcttgtttt ctaagagata aataaaggaa taatacaatg aacgatctat
    21181 tttcactggc aggaaaaaat atcttgatta ccggttcagc acagggcatt ggctttttac
    21241 tggcaaccgg cctgggtaaa tatggcgcac aaataattat taatgatatt actgccgaac
    21301 gcgcagaact tgctgtagaa aaactccacc aggagggtat tcaggccgtt gccgcacctt
    21361 ttaatgttac tcataaacat gaaattgatg ccgccgttga acatatcgaa aagaacatcg
    21421 gccccattga tgtgctggtg aataacgccg gtatccagcg ccgtcatcct tttactgagt
    21481 tccctgaaca agagtggaat gatgtgatcg cagtaaacca gaccgccgtg ttcctggtat
    21541 cgcaagcggt aactcgtcac atggttgaac gcaaggcagg taaagttatt aatatttgct
    21601 cgatgcaaag cgaactggga cgtgacacca tcacccctta tgccgcatcg aaaggggcgg
    21661 taaaaatgct cacccgcggc atgtgcgtcg agctggcgcg ccacaatatt caggtcaacg
    21721 gtattgcgcc gggctatttc aaaacagaaa tgactaaagc actggttgag gacgaagcct
    21781 tcaccgcctg gttgtgcaaa cggacccccg ccgcacgctg gggagatccg caggaactga
    21841 ttggtgctgc ggtgttcctt tcttcaaaag cctctgattt cgtaaacggc cacctgttgt
    21901 ttgttgatgg cggcatgtta gtggctgttt aacagccacc accctacgtg cagcgcgaaa
    21961 gcgctgcttt tctggcacta ataagagata aagactatgc cattaatcat tattgcggca
    22021 ggcgtcgcgc tgcttcttat cctgatgatc ggctttaaag ttaacggctt tattgccctc
    22081 gttctggtag ctgccgtcgt cggatttgcc gaagggatgg atgcacaggc cgtcctgcac
    22141 tctatacaaa atggtatcgg cagcacgctc ggcgggctgg caatgatcct cggtttcggg
    22201 gccatgttag gcaagctgat ttctgatacg ggcgcggcac aacgtatcgc cactacgctg
    22261 attgctactt ttggtaaaaa acgcgtgcaa tgggcgctag tgatcaccgg tctggttgtg
    22321 ggcctcgcca tgttttttga agtgggtttt gtcctgctgt tgccgttggt atttaccatc
    22381 gtagcatcat caggattacc cctgttgtat gttggcgtac caatggtagc agcgctctct
    22441 gtaacccact gttttctgcc gccacatcca gggcctactg ccatcgcgac tatctttgag
    22501 gctaatctcg gaacgacttt actgtatgga tttatcatta ccattccgac agttattgtc
    22561 gcaggaccgc tgttttctaa actgctaact cgctttgaga aagcaccacc ggaaggctta
    22621 tttaatcctc atctgtttag cgaaaaggag atgccctcct tctggaacag tattttcgct
    22681 gccgtgatcc cggtcatcct gatggctatc gccgccgttt gtgaaattac gttaccgaaa
    22741 actaacaccg tgcgcctctt ctttgaattt gtcggtaacc ctgccgttgc gctgtttatt
    22801 gccattgtta ttgcgatttt cacactgggc cgacgtaatg gacgcaccat cgagcaaatc
    22861 atggatatca ttggggattc tataggcgct atcgcgatga ttgtgtttat tatcgctggc
    22921 ggcggcgcgt ttaagcaggt attagtagat agcggtgtcg ggcagtatat ttcacactta
    22981 atgaccggaa ctacactttc gccgttattg atgtgctgga ctgttgcggc gctgttgcgt
    23041 atcgctctgg gctctgccac cgtcgcggcc attaccaccg cgggtgtggt gttgccgatt
    23101 atcaacgtta cccataccga tcccgcttta atggtactgg caaccggtgc gggcagcgtg
    23161 atcgcgtcac acgtaaacga ccctggcttc tggctattta aagggtattt taatctgacg
    23221 gttggtgaaa cgttgcgtac ctggacggtg atggaaaccc ttatttctat tatgggtttg
    23281 ctgggcgtgt tagccattaa cgccgtattg cactgaaaga ataaaatatt gtgatgtagt
    23341 ttcgggggaa gattgaagat tcccccgacg ttgggagaag ttatgcgcaa tcacagaatt
    23401 tctttacagg atatcgctac gctggctggc gtaacaaaaa tgaccgtgag tcgttatatc
    23461 cgctcgccga aaaaggtggc aaaggaaaca ggcgagcgca tcgccaaaat tatggaagag
    23521 atcaattata tccctaatcg cgctcccggc atgttgttga atgcacaaag ttataccctc
    23581 ggtatcttga ttccgtcatt tcaaaatcaa ctcttcgctg acattctcgc gggaatagaa
    23641 tccgtcacct ctgagcataa ttatcagacc cttattgcta attacaatta cgatcgcgat
    23701 tccgaagaag agtcggtgat taacctgctc tcttataaca ttgacgggat tatactttcg
    23761 gaaaaatatc acaccatcag gacggtgaaa tttctgcgtt cagccaccat tccggtcgtg
    23821 gaattgatgg atgtacaggg agaacggctg gatatggagg tcggttttga taatcggcag
    23881 gccgcttttg acatggtgtg taccatgctg gagaagcgag tcaggcacaa aattctgtat
    23941 ctcggttcca aagatgacac ccgcgatgaa cagcgttatc aggggtattg cgatgcaatg
    24001 atgctgcata atctttcccc attacgcatg aatccacgcg ccatctcatc cattcattta
    24061 ggaatgcagt taatgcgtga tgcccttagc gcaaacccgg atttagacgg tgtattttgc
    24121 accaatgacg atattgcgat gggtgcgctt ctgctgtgcc gcgagcgaaa cctcgccgtg
    24181 ccagaacaaa tttccattgc gggttttcat gggctggaga ttggcaggca aatgatccca
    24241 agcctcgcca gcgtaattac tccccgtttt gatattggtc ggatggccgc ccaaatgttg
    24301 ctgagcaaaa ttaagaataa cgatcacaac cacaacactg ttgacctcgg gtatcagatt
    24361 tatcacggta acacgcttta ggttattttc tctgcattac acatattctc tttgccggac
    24421 gttgcctata ctccaggcag gaaactggag gatatctcat gagcgaaccc ctgttaattg
    24481 cccgcacgtc ggacacagaa ctgtttttat tgccgggaat ggcgaatcgt cacgggctga
    24541 ttactggcgc aacggggacg ggtaaaactg tcacgttgca aaaactggca gagtcattgt
    24601 cggaaatcgg tgttccggtg tttatggccg atgtgaaagg tgatttaacg ggagttgcgg
    24661 agggaggtac atcttcggaa aaactgctcg caaggcttaa aaatatcggc gtaaatgact
    24721 ggcaacctca tgctaatccg gtggtggtgt gggatatctt tggcgagaaa ggccacccgg
    24781 tgcgggcgac ggtttcggat ctggggccgc tgttgctggc gcgactgttg aatctcaacg
    24841 atgtgcaatc tggcgtgctg aatattatct ttcgtattgc tgatgatcag gggctgttac
    24901 tgctcgactt taaagatctg cgggcgatta cccagtacat cggcgataac gccaaatcct
    24961 tccagaatca gtacggaaat atcagtagcg catcggttgg tgccatccag cgcggattac
    25021 tgtcgctgga gcaacaaggt gcggcgcatt tctttggcga gccgatgctg gatatcaaag
    25081 actggatgcg caccgatgct aacggtaaag gcgttatcaa tatcctcagc gccgagaagc
    25141 tttatcagat gccgaaactg tacgccgcca gcctgctgtg gatgctttca gagttgtatg
    25201 aacaattgcc ggaagcgggc gatctggaga aaccaaaact ggtgtttttc ttcgacgagg
    25261 cgcatctgct gtttaatgac gcaccgcagg tactgctgga taagattgag caggtgataa
    25321 ggcttatccg ctcgaaaggc gtgggcgtct ggttcgtttc gcaaaacccg tcagatattc
    25381 cggataacgt gctcgggcag ctcggtaatc gcgttcaaca cgctttgcgt gcttttacgc
    25441 ctaaagatca gaaagcagtg aaggcagcag cgcaaaccat gcgggccaat ccggcgtttg
    25501 ataccgaaaa ggcaatccag gaactgggga cgggcgaagc gttgatctcg tttctcgatg
    25561 caaaaggaag tccttcagtg gtggaacggg cgatggtgct cgcaccttgt tcgcggatgg
    25621 ggccggtgac ggaagatgag cgtaatggct tgattaatca ctccccggtg tatggcaaat
    25681 atgaggatga ggtggaccgc gagtctgcct atgagatgct acaaaaaggt gtgcaggcca
    25741 gtaccgggca gcaaaataat ccccccgcga aagggaaaga ggtggcggtg gatgacggta
    25801 ttcttggtgg attgaaggat attttgtttg gcactaccgg accacgcggc gggaagaaag
    25861 atggtgtggt gcagacaatg gcgaaaagcg ccgcccgcca ggtgacgaat cagattgtgc
    25921 gtgggatgtt ggggagtttg ctggggggga gaaaaagata agtcagacgt gatctgtgcc
    25981 cctcacccta accctctccc caaaggggcg aggggaccgt tcgaactcgt atttgacttt
    26041 gtcaacaata ataaagggag ctttcgctcc cttgttagat tacgattttc tcattaacag
    26101 ccacaggctg attaagaaga agctggcgct tggcaacagt gcgccaatga tcggcgggat
    26161 gccataaacc aacgtcagcg ggccgaagat ctggtccagt acgtagaaaa caaaaccgaa
    26221 gctgataccg gtgaccacac gcacgcccat cggtacgcta cgcagtgggc caaagatgaa
    26281 cgacagcgcc atcaacatca tcaccgccac ggatagcggc tggaagattt tgctccacat
    26341 gttgagctga taacgtccga catcctgacc gctcgacttc agatacttca catagttgtg
    26401 caaaccactg atggagagtg catccgggtc cagcgccacc acgcccagtt tgtctggcgt
    26461 gaggttggtt ttccaggtgc cgctcaccgt ctgcaaaccg gtgatctgtt tcggattggt
    26521 cagatcagat tcatcaacct gcgacagacg ccagacttta tgttccgggt caaacttcgc
    26581 ggtagcggca tagcgtacgg attgcagacg acgattctcg ttaaaggcat aaatactgat
    26641 gccacctaac tcttcgtcac ctttaacccg ctcaatgtag acgaagttgt tgccatcttt
    26701 cgcccataag ccttgctgag tggagagcaa cgagccgccg tacatcgcct gcgcacggta
    26761 gttacgcgcc atctgttcgc cctgcggcgc gacccattcg ccaatcgcca tcgtcagcaa
    26821 gaccagcgga atggcggttt tcatcaccga cagcgcaacc tgcatacggg taaaaccaga
    26881 agcctgcatc accaccagtt cgctgcgctg cgccagcatc ccaagaccaa gcaacgcccc
    26941 aagcagagcc gccatcggga agaagatctg cacatctttc ggcacgctca gcaaggtata
    27001 cattcctgcg cctaacgcgt cgtaactccc ctgcccggct tttttcagtt gatcgacaaa
    27061 tttgataatg cccgacagcg acaccagcat gaacagcgtc atcatgatgg tggtgaaaat
    27121 agttttaccg atatagcggt caagtacgcc aaaaggttgc atcacaccgc tcctttacgc
    27181 gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag aacaatcgcc
    27241 aaagccagat aaatcaggtt aacggtccac atccacagcg tcgggtccag cttaccttta
    27301 ccaccgttcg atttcaggga ggtctggatc aggaagaaaa gtagatacag cagcatggct
    27361 ggcagcatcg acagtacgcg tccctgacgc gggttaacca cgctcagcgg tacgaccata
    27421 agtgccatca taaacacggt gaataccaac gtgatacgcc agttcagttc tgcgcgggca
    27481 cgatcggtgt cagtgttcca caatgtgcgc atgtccatct ggtcggtatc gttcgggtcg
    27541 agcgccaccg cctggtgacc aatgatcgcc tgataatcct ggaagtccgt aatgcggaaa
    27601 tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac gacctgggag
    27661 ccgtcgcgca gctgggttaa atgtccggaa tcggccacca ccacagaagg acgtgcatta
    27721 ccttttggtc gaatttgtgc gaggaacaca tctttgaaat cgctgccgtc aacgctttcg
    27781 atgaacagca ccgagctgcc attagtcgct tgctggaatt gcccttgcgc cagcgccgcc
    27841 atgccagggt tcgctttcgc ttctgctaac acttcatcct gatgacgcga tgaccacggt
    27901 cccgcccaca tcacgttaac cgccgcgacg attgccgtga ataccgcaag gatcattgcc
    27961 gctttcacca gaaccgcttt gctcaagccg caggcatgca ttaccgtaat ttcactttcg
    28021 gtatacagtt tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg caggataagc
    28081 tgcgccattt ccggcacgcc caacccgaga agggagagca ccagattcgc cggaatatcg
    28141 ccgtcaaccg ctgcgccgag gatcctcact aacttttgac agaagaagat caaaagcaag
    28201 atgaagagta tcgccagctg gcttttgagc gtctcccgca ccagatatct tatgattatc
    28261 actttaaata cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt tcatggctta
    28321 aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa gataattata ctcaacggat
    28381 tcacctctca gattttgttc tgacgtgcca atgccgtaat aacgttaaga ttaacacgaa
    28441 gtcatcgcaa cagcggacat gagttacgaa agcttgcaat tctatctgta gccaccgccg
    28501 ttgtctttaa gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg gtagcccgga
    28561 gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc tttctccgat
    28621 tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc gccctgctac gtcggggcga
    28681 actggaagga aaaccggggc agacactgtt gctgcaccat gtgccaaacg tgctttctga
    28741 acgcatcctc cttataggtt gcggcaaaga acgtgagctg gatgagcgtc agtacaagca
    28801 ggttattcag aaaaccatta atacgctgaa tgatactggc tcgatggaag cggtctgctt
    28861 tctgactgaa ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc aggctgtcga
    28921 gacagcaaaa gagacgctct acagtttcga tcagttgaaa acgaataaga gcgaaccgcg
    28981 tcgcccgctg cgtaagatgg tgttcaacgt gccgacccgc cgtgaactga ccagcggtga
    29041 gcgcgcgatc cagcacggtc tggcgattgc cgccgggata aaagcagcga aagatctcgg
    29101 taatatgccg ccgaatatct gtaacgccgc ttatctcgcc tctcaggcgc gccagctggc
    29161 tgacagctac agcaagaatg tcatcacccg cgttatcggc gaacagcaga tgaaagagct
    29221 ggggatgcat tcttatctgg cggtcggtca gggttcgcag aacgaatcgc tgatgtcggt
    29281 gattgagtac aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg tgggtaaagg
    29341 tttaaccttc gactccggcg gtatctccat caagccttcc gaaggcatgg atgagatgaa
    29401 gtacgatatg tgcggcgcgg cggcggttta cggcgtgatg cgtatggtcg cggagctgca
    29461 actgccgatt aacgttatcg gcgtgctggc aggctgcgaa aacatgcctg gcggacgtgc
    29521 atatcgtccg ggcgatgtgt taaccaccat gtccggccaa accgtagaag tgctgaatac
    29581 cgacgccgaa ggccgtctgg tactatgcga cgtgttaact tacgttgagc gttttgagcc
    29641 ggaagcggtg attgatgtgg cgacgctgac cggtgcctgc gtgatcgcgc tgggtcatca
    29701 tattactggt ctgatggcga accataatcc gctggcccat gaactgattg ccgcgtctga
    29761 acaatccggt gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg aacaactgga
    29821 gtccaatttt gccgatatgg cgaacattgg cggtcgtcct ggtggggcga ttaccgcagg
    29881 ttgcttcctg tcacgcttta cccgtaagta caactgggcg cacctggata tcgccggtac
    29941 cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgc ccggtggcgt tgctggcaca
    30001 gttcctgtta aaccgcgctg ggtttaacgg cgaagagtaa gtttgcgttt taccctcacc
    30061 ctaaccctct ccctaaggga gagggaaccg atcgagcgca atagaaattc attgctcatc
    30121 tttcagggtg aggaatgcag caatatttaa atccaccaca agaagcccca tatatgaaaa
    30181 acgcaacgtt ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc
    30241 aactggtgtg tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgtg ctcatcgcct
    30301 gtgaagatga aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa
    30361 gctttgttcc gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg
    30421 cctggccgca aaagcgtagc agcagcccgc gcgatatatt gattagtctg cgaacaagct
    30481 ttgcagattt tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc
    30541 tgaaacaact ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata
    30601 cggcaacctg gaaataatgg aaaagacata taacccacaa gatatcgaac agccgcttta
    30661 cgagcactgg gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt
    30721 ctgcatcatg atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca
    30781 gcaaaccatc atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg
    30841 gcaggtcggt actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc
    30901 cgcagaagaa ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg
    30961 ggaatggaaa gcggagtccg gcggcaccat tacccgtcag atgcgccgtc tcggcaactc
    31021 cgtcgactgg gagcgtgaac gcttcaccat ggacgaaggc ctgtccaatg cggtgaaaga
    31081 agttttcgtt cgtctgtata aagaagacct gatttaccgt ggcaagcgcc tggtaaactg
    31141 ggacccgaaa ctgcgtaccg ctatctctga cctggaagtg gaaaatcgcg aatcgaaagg
    31201 ttcgatgtgg cacatccgct atccgctggc tgacggcgcg aaaaccgcag acggtaaaga
    31261 ttatctggtg gttgcgacca ctcgcccgga aaccctactg ggcgataccg gcgtagccgt
    31321 taacccggaa gatccgcgtt acaaagattt gattggcaaa tatgtcattc tgccgctggt
    31381 taaccgtcgt attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg
    31441 cgtgaaaatc accccggcgc acgactttaa cgactatgaa gtcggtaaac gtcacgcgct
    31501 gccgatgatc aacatcctga cctttgacgg cgatatccgt gaaagcgccc aggtgttcga
    31561 taccaaaggt aacgaatctg acgtttattc cagcgaaatc ccggcagagt tccagaaact
    31621 cgagcgtttt gctgcacgta aagcagtcgt tgctgcggtt gatgcactcg gcctgctgga
    31681 agaaattaaa ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tggttatcga
    31741 accgatgctg accgaccagt ggtacgtgcg tgccgatgta ctggcgaaac cggcagttga
    31801 agcggttgag aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc
    31861 ctggatgcgc gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat
    31921 cccggcatgg tatgacgaag cgggtaacgt ttatgttggc cgcaacgaag acgaagtgcg
    31981 taaagaaaat aacctcggtg ccgacgttgc cctgcgtcag gacgaagacg ttctcgacac
    32041 ctggttctct tctgcgctgt ggaccttctc tacccttggc tggccggaaa acaccgacgc
    32101 cctgcgtcag ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg
    32161 gattgcccgc atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca
    32221 ggtgccgttc cacaccgttt acatgaccgg cctgattcgt gatgacgaag gccagaagat
    32281 gtccaaatcc aagggtaacg tgattgaccc gctggatatg gttgacggta tttcgctgcc
    32341 ggaactgctg gaaaaacgta ccggcaatat gatgcagccg cagctggcgg acaaaatccg
    32401 taagcgcacc gagaaacagt tcccgaacgg cattgaaccg cacggcaccg atgccctgcg
    32461 cttcaccctg gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgcct
    32521 ggaaggttac cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa
    32581 cacagaaggt caggattgcg gcttcaacgg cggcgaaatg acgctgtcgc tggcggatcg
    32641 ctggattctg gcggagttca accagaccat caaagcgtac cgcgaagcgc tggacagctt
    32701 ccgcttcgat atcgccgcag gcattctgta tgagttcacc tggaaccagt tctgtgactg
    32761 gtatctggag ctgactaagc cggtaatgaa cggtggcacc gaggccgaac tgcgcggtac
    32821 tcgccatacg ctggtgaccg tgctggaagg tctgctgcgc ctcgcgcatc cgatcattcc
    32881 attcatcacc gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac
    32941 catcatgctg caaccgttcc cgcagtacga tgcgtctcag gtggatgaag cggcactggc
    33001 cgacaccgag tggctgaaac aggcgatcgt tgctgtacgt aacatccgtg cagaaatgaa
    33061 catcgcgccg ggcaaaccgc tggagctgtt gcttcgtggt tgcagcgcgg atgcagagcg
    33121 tcgcgtaaat gaaaaccgtg gcttcctgca aaccctggcg cgtctggaaa gcatcaccgt
    33181 gctgcctgcc gatgataaag gtccggtttc cgttacgaag atcatcgacg gtgcagagct
    33241 gctgatcccg atggctggcc tcatcaacaa agaagatgag ctggcgcgtc tggcgaaaga
    33301 agtggcgaag attgaaggtg aaatcagccg tatcgagaac aaactggcga acgaaggctt
    33361 tgtcgcccgt gcaccggaag cggtcatcgc gaaagagcgt gagaagctgg aaggctatgc
    33421 ggaagcgaaa gcgaagctga ttgaacagca ggctgttatc gccgcgctgt aatcaacacc
    33481 gtgat
//
LOCUS       DAOTRK010000042        33052 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.042, whole genome shotgun sequence.
ACCESSION   DAOTRK010000042 DAOTRK010000000
VERSION     DAOTRK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.042"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            20..1069
                     /locus_tag="QIE41_001900"
     CDS             20..1069
                     /locus_tag="QIE41_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDS3530792.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1143..2000
                     /gene="nfo"
                     /locus_tag="QIE41_001901"
     CDS             1143..2000
                     /gene="nfo"
                     /locus_tag="QIE41_001901"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDS3530793.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2003..3091
                     /locus_tag="QIE41_001902"
     CDS             2003..3091
                     /locus_tag="QIE41_001902"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDS3530794.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNTAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3147..4397)
                     /locus_tag="QIE41_001903"
     CDS             complement(3147..4397)
                     /locus_tag="QIE41_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDS3530795.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4497..5438)
                     /gene="rihB"
                     /locus_tag="QIE41_001904"
     CDS             complement(4497..5438)
                     /gene="rihB"
                     /locus_tag="QIE41_001904"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDS3530796.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5568..6266
                     /gene="yeiL"
                     /locus_tag="QIE41_001905"
     CDS             5568..6266
                     /gene="yeiL"
                     /locus_tag="QIE41_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDS3530797.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6334..7584)
                     /locus_tag="QIE41_001906"
     CDS             complement(6334..7584)
                     /locus_tag="QIE41_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDS3530798.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKGISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFTNASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7678..8616)
                     /gene="psuG"
                     /locus_tag="QIE41_001907"
     CDS             complement(7678..8616)
                     /gene="psuG"
                     /locus_tag="QIE41_001907"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDS3530799.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(8604..9545)
                     /locus_tag="QIE41_001908"
     CDS             complement(8604..9545)
                     /locus_tag="QIE41_001908"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDS3530800.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSVPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(9969..11660)
                     /gene="fruA"
                     /locus_tag="QIE41_001909"
     CDS             complement(9969..11660)
                     /gene="fruA"
                     /locus_tag="QIE41_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDS3530801.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(11677..12615)
                     /gene="fruK"
                     /locus_tag="QIE41_001910"
     CDS             complement(11677..12615)
                     /gene="fruK"
                     /locus_tag="QIE41_001910"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDS3530802.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12615..13745)
                     /gene="fruB"
                     /locus_tag="QIE41_001911"
     CDS             complement(12615..13745)
                     /gene="fruB"
                     /locus_tag="QIE41_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDS3530803.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14113..15294
                     /gene="setB"
                     /locus_tag="QIE41_001912"
     CDS             14113..15294
                     /gene="setB"
                     /locus_tag="QIE41_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDS3530804.1"
                     /translation="MHNSPAVSSAKSFDLTSMAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15291..15545)
                     /locus_tag="QIE41_001913"
     CDS             complement(15291..15545)
                     /locus_tag="QIE41_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDS3530805.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15700..16272
                     /gene="yeiP"
                     /locus_tag="QIE41_001914"
     CDS             15700..16272
                     /gene="yeiP"
                     /locus_tag="QIE41_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDS3530806.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16495..17961
                     /locus_tag="QIE41_001915"
     CDS             16495..17961
                     /locus_tag="QIE41_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDS3530807.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKRADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLAPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            18079..19065
                     /gene="yeiR"
                     /locus_tag="QIE41_001916"
     CDS             18079..19065
                     /gene="yeiR"
                     /locus_tag="QIE41_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDS3530808.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNERWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHHHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19104..19817
                     /gene="lpxT"
                     /locus_tag="QIE41_001917"
     CDS             19104..19817
                     /gene="lpxT"
                     /locus_tag="QIE41_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDS3530809.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20229..20795
                     /gene="mepS"
                     /locus_tag="QIE41_001918"
     CDS             20229..20795
                     /gene="mepS"
                     /locus_tag="QIE41_001918"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDS3530810.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            20976..22532
                     /locus_tag="QIE41_001919"
     CDS             20976..22532
                     /locus_tag="QIE41_001919"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDS3530811.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQSLRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22614..24428
                     /locus_tag="QIE41_001920"
     CDS             22614..24428
                     /locus_tag="QIE41_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDS3530812.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPLARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKNHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            24429..25523
                     /locus_tag="QIE41_001921"
     CDS             24429..25523
                     /locus_tag="QIE41_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDS3530813.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25523..26548
                     /locus_tag="QIE41_001922"
     CDS             25523..26548
                     /locus_tag="QIE41_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3530814.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLEDKGWVLWAPIRFGA
                     TSINFSTDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26550..28139
                     /gene="yejF"
                     /locus_tag="QIE41_001923"
     CDS             26550..28139
                     /gene="yejF"
                     /locus_tag="QIE41_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDS3530815.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(28143..28487)
                     /locus_tag="QIE41_001924"
     CDS             complement(28143..28487)
                     /locus_tag="QIE41_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530816.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28820..30010)
                     /gene="bcr"
                     /locus_tag="QIE41_001925"
     CDS             complement(28820..30010)
                     /gene="bcr"
                     /locus_tag="QIE41_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDS3530817.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(30038..30733)
                     /gene="rsuA"
                     /locus_tag="QIE41_001926"
     CDS             complement(30038..30733)
                     /gene="rsuA"
                     /locus_tag="QIE41_001926"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDS3530818.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30883..32643
                     /locus_tag="QIE41_001927"
     CDS             30883..32643
                     /locus_tag="QIE41_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDS3530819.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32768..33052
                     /gene="rplY"
                     /locus_tag="QIE41_001928"
     CDS             32768..33052
                     /gene="rplY"
                     /locus_tag="QIE41_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDS3530820.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
BASE COUNT         8128 a         8581 c         8157 g         8186 t
ORIGIN      
        1 ccggttaaag gagagggtta tgacgaatat caccttacag aaacaacatc gtacactgtg
       61 gcattttatt ccggggttag ccctgagtgc agttatcacc ggggtcgccc tgtggggtgg
      121 ttccattccc gcggttgcgg gtgccgggtt tagtgccctc accctcgcaa ttttgttggg
      181 gatggtttta ggcaacacca tctatccgca catctggaaa agctgtgacg gtggtgtgct
      241 gtttgccaag caatatttat tacgtctggg tatcattctt tatggcttcc gtctgacgtt
      301 ctcgcaaatt gccgatgtcg gtatcagtgg gatcatcatt gacgtgctga cgctgtccag
      361 taccttcctg ctggcttgct tcctggggca gaaagtgttt ggtctggata agcacaccag
      421 ctggttgatc ggtgccggta gcagtatttg tggtgctgcc gcggtactgg cgactgagcc
      481 ggtagtgaaa gcggaagcca gtaaagtaac cgtggctgtt gcaaccgttg ttatcttcgg
      541 gaccgtcgcg attttcctct acccggcgat atatccgctg atgtctcaat ggtttagtcc
      601 ggaaactttc ggtatctaca ttggttctac tgtgcacgaa gtggcgcagg tggtggcggc
      661 aggtcatgcc atcagcccgg atgcagaaaa cgcagcagtt atttccaaaa tgctgcgcgt
      721 gatgatgctg gctccgttcc tcatcctgct ggcggcgcgt gttaaacagc tgtctggggc
      781 gaacagcggc gagaaaagca aaatcactat tccgtggttt gccatcttgt tcatcgtagt
      841 tgccatcttt aactcgttcc acctgttacc gcagagcgtg gtgaacatgc tggtaacgct
      901 ggataccttc ctgctggcaa tggcgatggc ggcgctgggt ctgaccactc acgtcagcgc
      961 gctgaaaaaa gccggggcga aacctctgct gatggcactg gtactgtttg cctggctgat
     1021 tgttggtggt ggtgcgatta actatgtgat tcaaagcgtc atcgcataaa ccactacatc
     1081 ttgctcctgt taacccgcta tcattaccgt tttcctccag cgggtttaac aggagtcctc
     1141 gcatgaaata cattggagcg cacgttagtg ctgctggcgg tctggcaaat gccgcaattc
     1201 gcgccgccga aatcgacgca accgcgtttg ccttgttcac caaaaaccaa cgtcagtggc
     1261 gtgccgcacc gctcacgacg caaaccatcg atgaattcaa agccgcctgt gaaaaatatc
     1321 actacacatc ggcgcaaatt cttccccacg acagttatct gattaacctc ggacatccgg
     1381 tcgctgaagc tctggaaaaa tcgcgcgatg cgtttataga tgaaatgcag cgttgcgaac
     1441 agctggggct ttctttgctc aacttccacc ctggcagcca tctgatgcag atttcagaag
     1501 aggattgcct tgcgcgtatt gccgaatcca tcaacattgc gctggataaa actcaaggtg
     1561 tgacagcggt gattgaaaac accgccggtc agggcagtaa cttagggttt aaattcgaac
     1621 atctcgcggc gattatcgac ggcgtggaag ataaatcccg cgtcggcgtc tgcattgata
     1681 cctgccatgc ttttgctgcc gggtatgatt tgcgtactcc agccgaatgc gagaaaacgt
     1741 tcgcggattt tgcccgtatt gtcggcttta agtatctgcg cgggatgcac cttaacgatg
     1801 cgaaaagcac ctttggcagc cgcgttgacc gccatcatag cctcggtgaa ggcaatatcg
     1861 gtcatgatgc gttccgctgg atcatgcagg acgaccgttt cgacggcatt ccgctgatcc
     1921 ttgaaaccat caacccggat atctgggcag aagagatcgc ctggctgaaa gcgcaacaaa
     1981 ctgaaaaagc ggtagcctga agatgaacaa ccgggagaag gagatccttg caattttacg
     2041 gcgtaacccg ctgattcagc agaacgaaat tgctgatatg ctgcaaatca gccgttcgcg
     2101 cgttgcggcg catattatgg atttaatgcg caaaggtcgg attaaaggca aaggttacat
     2161 tctcaccgag caggaatact gcgtagtggt ggggacaatc aatatggata ttcgcgggat
     2221 ggcggatatc cgttacccgc aagcggcttc tcatcccggt acaattcatt gctcggcagg
     2281 cggcgttgga cgcaatatcg cccacaatct ggcgctgtta ggccgcgacg tccatttgct
     2341 ttcagtgatt ggcgatgact tttatggcga aatgctcctg gaagaaacgc gccgcgccgg
     2401 cgtgaatgtc tccggctgcg ttcgtttaca tggtcaaagc acatcgacgt atctggcaat
     2461 tgccaatcga gacgatcaaa ccgtgctggc gattaacgat acccatctgc tggaacagtt
     2521 gacaccgcaa ctactgaacg ggtcgcgcga tttacttcgt catgcgggcg tggtactggc
     2581 tgattgcaac ctgacagccg aggcgctgga gtgggtcttt accctcgctg atgaaatccc
     2641 ggtgtttgtc gataccgttt cagaattcaa agcgggcaaa atcaaacact ggctggcgca
     2701 tattcacacc ctgaaaccca ctttaccgga gctggaaatt ttatggggac aggcgatcac
     2761 cagcgatgct gaccgtaata ccgcagtgaa tgcgttgcat cagcaaggcg ttcagcaact
     2821 gtttgtttat ttgcccgatg agtcagttta ttgcagcgaa aaggatggag aacaattttt
     2881 gctgaccgca ccagcgcata cgacagtaga cagttttggt gctgacgatg gttttatggc
     2941 gggcctggta tatagctttc tggaaggaaa caatttccgt gacagcgccc gttttgcgat
     3001 ggcctgcgcg gcaatttcac gcgccagcgg cagcttaaac aaccctaccc tgtctgccga
     3061 taacgcgctt tcattagtgc cgatggtgta acaatcttgc cggattcgac gcttgaccgc
     3121 gtcttatccg gctcataaat cgcgcactac gctaagccaa taaagaatcc tgcaatggtg
     3181 gcactcatca ggttagaaag tgtcgccgcc gccagcgcgc gtaaaccaag ctgggcgatt
     3241 tccggcgcac ggtgtggcgc aaccgcagaa aacgccccca ccaccacccc gatagaacca
     3301 aagttagcga aaccgcacaa cgcgaaggaa ataatcgcca cggttttagc atccagagtg
     3361 ccagctgttt gcagataagg tgagaaattg aggtaagcga caaattcatt aatcgccagt
     3421 ttctgtccaa tcaaactccc ggcaagattc gcatcactcc agtcaacccc catcacccac
     3481 gccagtggag ccaacaggta acctaaaatg gactccagcg aggcatgttc aaaaccaaac
     3541 cagccgccaa cgccgccgat aataccgtta atcaacgcaa ttattgcaac aaatgccatc
     3601 accactgtcg ccacacctgc ggcgattttc agtccggtca tcgcaccgct cgccgctgct
     3661 tcgataaagc tttttggcgg agtttcggtg aaagagaggt tattaaagga aacctgcgaa
     3721 gattcggtag ccgggcttaa caggcgggca aacaagatcc cgccggggat cgccattaac
     3781 gatgccgcca gcagatattc cacaggcacg cccagtgcag cgtaaccaat cattgtcgaa
     3841 ccagcaatcg aggccatgcc actacaaatc gctgtaaata attcattgcg attcagccga
     3901 tcgataaagg gtttgacgat tgccggaatt tcgttttgcc cgaggaaaat ggtagtgacc
     3961 gcgacgaatg actcgatctt gctgatattt aatgctttct ggaagatacc gccgagaatg
     4021 cgaattaaga tccccatcac accgatgtag tagagaatac tcaccagtgc tgtaacgaag
     4081 ataattgccg gtaataccct gaaaccaaag ataaatcctg cgccatcaaa taaggtatcc
     4141 attttcggtc cgaccagaga accaaagata aatgcgctac ctgcatcgct gtacgccatc
     4201 actttatgca cgccaaaagc gactttttct gcgacccaac gccctggtgg taaccaaagc
     4261 ataatgccgc caatcacgac ctgtaacact aacgccgcgc caacggtacg caggctgatc
     4321 ttcttcttgt ttactgacag caaaaacgca attgccagca atacaaccat tcccagaaca
     4381 cttctcatga catccatagt tattatctct tcatgtcatt ttgggcgacg gctttctgcc
     4441 catcagaaat agccgggcct gcatcttgcg ataccggccc ggcttgttat caacacttaa
     4501 tgggttttga tgtagccgcg cacgcactct tcgactaagc cccagaacca gtcggtatca
     4561 atagtgatgc cgactttggt attggctggc ttgccaagaa cgcccagctc gtcgcagacg
     4621 gtacgcccat agcaagggcc actgttaaca tccacttcga cgtacatatc ctgggtttta
     4681 atgccatcag ggttgatcag ataaccgatg caggtggcgt cgtgcaccgg gccgccagcc
     4741 aggccatagt tttcgaactg ggttttgaga gtgaagttca tgatgtcgct gaacagctct
     4801 ccggcagggc cgcctgccct ttccatccgg gcaatcacgt ccggggtgca aacggtctgg
     4861 ttggtgagat cgaggcccat catcaccaac ggaacgccgg aggtgaacac cacgcgtgcg
     4921 gcttccgggt cagcaaagat gttgaactcg gcggatggcg taaagttgcc agtaccgtaa
     4981 gcgccgccca tcagcacaat ttcacggatt ttcggcagga ttgcgggctg catgcgcatt
     5041 gccaccgcga tatttgaaag cggaccaacc ggcaccagag tgatatcgcc atcgcttgcc
     5101 atcagggtat cgatgatata tttcaccgca tgagtgcttt ctgcctggcg ggtcagcggc
     5161 tcgaataccg ggccatccag tccggtttca ccgtgaatat tatcggcaac gatttgttga
     5221 cgcataatgg gctgtggcat ccccgcataa accggaacat taatctccag tttctggcaa
     5281 acattcaggc cattaattaa tgttttatca agcgtctgat tacccgccac aatggtgatg
     5341 cctaataaat ctattgccgg atgtttcgct gccatcatta tggcaatagc atcatcatga
     5401 cccggatcac aatccagaat aatttttctc ttttccattg tttatttcct ctgtttccag
     5461 ttgcgttatt ttttctacag caaagaaaat tcgaccgggc cgatgattga atcttaacaa
     5521 cagcgtaagt atgctaaata tgagaaatct catagcggat aaacatcgtg aaagaaatcc
     5581 acaataatga ccttaagcag caattgatga gtgaatccgc gtttaaggat tgcttttcaa
     5641 cggatgtttc agccgatacg cggctgtttc attttttagc gcgtgactac attgtgcagg
     5701 aagggcaaca gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg ctttacgcca
     5761 cgctagctaa tggtcgcgtg tcgctgatcg atttctttgc cgctccctgt tttattggcg
     5821 agattgagtt aatcgacaaa gaccatgaac cgcgtgcggt acaggctatt gaagagtgtt
     5881 ggtgccttgc gctccctatg aaacattacc gtccgctgtt attaaacgac acgctatttt
     5941 tacgaaaact ctgcgtcacc ttaagccata aaaattatcg caatattgtt tctttaactc
     6001 agaatcaatc atttccgtta gtgaatcgcc tggcagcttt tatattactc tcgcaggaag
     6061 gtgatcttta tcacgaaaag catacgcaag cggcagagta tttaggcgtt tcttatcgac
     6121 atcttttata tgttctagcg caattcattc acgacggttt attaattaaa agcaagaaag
     6181 ggtatctaat taaaaacaga aagcagttgt caggactggc actggagatg gacccggaaa
     6241 ataaattctc agggatgatg cagtaaaaat tatttgcaat agagcgattg ccggatgtga
     6301 cgctaaataa gcgcgtacat ccggcaatga aactcaagct aaaccaataa agaacccggc
     6361 aatggtcgcg ctcatcaggt tggaaagcgt tgctgctgcc agcgcccgta aaccaagctg
     6421 ggcgatttcc ggtgcgcgtt taggcgaaat agccgaaaat gcgccaacga caacaccgat
     6481 agaaccaaag ttagcaaaac cacaaagcgc aaaggagata atcgcaatgg ttttcacttc
     6541 cagcgtgccg cccgtttgca ggtatgggga gaaactcagg taagcgacga attcgttaat
     6601 cgccagtttc tgcccaatca ggctacccgc aagattggca tcactccagt ccacacccat
     6661 gatccacgcc agcggtgcca gcacatagcc aaaaatactt tccagagaag cattggtaaa
     6721 accaaaccag ccgccaattc cgccgataat gccattaatc agcgcaataa ttgcgacaaa
     6781 cgccattacc accgtcgcca caccagcggc gattttcaac ccggtcatcg caccgctcgc
     6841 cgctgcttcg ataaagcttt ttggcggtgt ttcgctgaac gacagatttt caaatgtgac
     6901 ctgcgaaggc tcggttgccg ggctaagaat acgtgcaaac aaaatcccgc cagggatcgc
     6961 catcagcgat gccgctaaca ggtagtcaat tggtacgccc attccggcat aaccaatcat
     7021 catcgaacca gcaatggacg ccatcccgct acaaattgcg gtaaacaact cgttgcgatt
     7081 catgcgatcg ataaacggtt taacgatcgc cgggatctca ttttgcccga ggaaaatagt
     7141 ggtaaccgca acaaaagatt cgattttgct gatgttgaga gctttctgga aaatgctgcc
     7201 aagaatgcga atcagcagtc ccatcacgcc aatgtagtac agcaggctga tgagcgcagt
     7261 gacgaagata atcgccggga gtacgcgaaa ggcgaagata aagcccgcgc catcaaacag
     7321 gacatccatt ttcggcccga ccaatgaacc aaaaataaag gcgctaccgg catcactgta
     7381 agacatcact ttatgaacgc ctaatgccgc ctgttctact gcccattttc ccggtgggaa
     7441 gtagagcatg atgccaccaa tagcgatttg cagcagcagt gcggctccaa ccgtgcgcaa
     7501 actgatgccc tttttattca ctgacaacag aaatgctatt gccagtaaca ccaccatccc
     7561 cacaacactt ctcattatat ccataatgat tttcccttca tgccagtaaa cccggcgtca
     7621 gcgccaggtt ttggtatgct tgatgagtac gggcgacggc tttctgcccg tcagaaatta
     7681 acccgcgaga cgctgatatt ctttggcaat ttcgctcgcc agaatggcgt tgttgaacac
     7741 cagctggatg ttggatttca ggctgtcacc gccagtcagt tcagcaacac gagccagcag
     7801 gaacggtgtg ctttctttac caataacgcc ctgctcttca gcttcagcta ccgcctgatc
     7861 gatcgccgca ttgatagtgt gttccggcat cgcaaactgt tccgggatcg gatttgccac
     7921 caccaggcca ccgttcagac cgctttgcca tttcaccgcc attgcacgag cgatttccgt
     7981 ggcactatca aggcgaatac taacttcaaa tgggctggta cggcagaaaa acgcaggcag
     8041 cgcttttgtc tgatagccaa ttaacggcac accgaaggtt tctaaatatt ctgttgttaa
     8101 tcctaaatcg agaatagatt ttgcaccggc acaaacaacg gtgacattag tatttgccag
     8161 ttcttgcaaa tcggcagaaa tatcgaaggt atgttccgcc ccgcgatgca caccaccaat
     8221 tcccccggtg gcaaatactt taattccggc aagcgccgca ataatcatcg ttgaagcaac
     8281 ggtagttgcg ccattttttc cggcggcaac aacaaaaggt aaatcgcgac gactaacttt
     8341 ggtcacgtta tgcccttcac gacccagcaa ttcaatttct tctttgctta aacccacttt
     8401 catcacgccg ccaataatgg cgatagtggc aggtacagcg ccctgtttac gaatagtttc
     8461 ttcaacttca attgcggtct gggcattttg tgggaacggc atcccgtgag aaataatggt
     8521 cgattccagc gccacaaccg gttttttgtt ttttaaagcg tcctgcactt ccggggaaat
     8581 ttgtaataat tcaggggaaa tttttaattc agacattctg cgttctccac taacgatata
     8641 acgttggcaa tcgataaatc ggggttattg gtgtattcac aggagagcgc cattgacgaa
     8701 catccctgtg cgaaacgaac agattcggca aacggcattc cgtctaccca acacgaagca
     8761 agtccagcca tcatggcatc gcccgctccg gtaacattaa tgacattggt tttgatcggc
     8821 acagaccaac cactttcacc gctgatatca ctgtaataaa cgccgtcgcc gcccatgctc
     8881 aataccagtc ggttcaggcc atgttgatgg aaccaggcag caacttttgc cacatcttca
     8941 cgccctgaca aagcaatccc actcagggtt tccgcttcaa ggcggtttgg cttgagagtg
     9001 tggatttgat ttagacggtc gcgcactttg acacatttcc acgcggaaac aggatcgaca
     9061 aatacgggta cgttggcggc attatccaga atccatgcca gtgcctcttc actgatatta
     9121 cagtccgcga caatgacctt tgccctctga ataaattcac cgtgctgtgc gagatattca
     9181 gctgtaatag cgttgctaat attcatgtca tttatagcaa ccagcatttc accggtatta
     9241 tcgagtaatg ataaatagct cgacgtattt tctcccggca caatcaggca tttatcgaca
     9301 taaacaccag attgattggt ttgcgttagc aacgattgac cataaaaatc actgcctact
     9361 gcgctcagta gccaggcttt attccccagc aacgccaggt tttgtgcaat attgcgccct
     9421 actccaccag gcgtaaattt tattttacct ggatttgaat ccgcataatt taatgattca
     9481 tgtgaatatc cggcgacatc aatattcgcc gaacctataa ttacgacata atccttttcg
     9541 cgcatagcac gtcctctggt aaaaaattac caaccctgtc aatccacaag aatattgata
     9601 ctgcaaagac ggtttaaaca tgtgttcata tttaaacatg tgctcatagt atgcatgttt
     9661 tacgtaaagt aaattaccgc cagagaggca aaatgggcag atttgagcag gctcacaaaa
     9721 cgcgattaaa tacctgaatt ataaaaaggg ggaatttgtg acgggatgca cagaacagga
     9781 acactaaaaa agggcagaaa ttaatctgcc cttgaggaaa taaccttagt gtttgatgaa
     9841 agtttcaggt ttccactaaa agataaaacc atttaaattc ataaaattac attcacaaac
     9901 cggtcattct ctttgcgcgc atcaaatgtt acagggcagg aaatttctgc cctgtaacac
     9961 accttttatt acgctgcttt cgctactgcg tccacttccg gacgtttcag gaaggcatag
    10021 gccagacccg ccaccagcgt accggcaata atggctacca ggtaacccaa taccggcgta
    10081 atcgcgccag ggatcagcag aacaaacaga ccaccgtgcg gtgccatcag tttcgcacca
    10141 atcgccattg agattgcgcc agtcagcgcc ccacccacga tacagcacgg cagcactcgc
    10201 atcggatcac gggcagcaaa cggaattgca ccttccgaaa tgaagcacag tcccaatacc
    10261 agagcggctt tgccaccttc ctgctgcgct ttgtcgaatt tacgacgcgc caccattgtt
    10321 gccaggccca ttgccagcgg tggcaccata cctgccgcca taatcgccgc catcgggcca
    10381 taggtttgag tactcagcag acccacaccg aacgcgtacg ctgctttgtt taccggaccg
    10441 cccatgtcag tacacatcat gccaccgagg atcgccccca gcagaaccgc attcgcagtc
    10501 cccatggtct gcagccagtg agtcaaccct tcgagaatgc cagcaaccgg tttaccgatc
    10561 aggtagatca tcgccagacc gaccaccaga ctggaaatta gcggaatgat cagaatcggt
    10621 ttcagcgcct ccatactctg tggcagtttc aattgcgtac tgattaactt cgcaatgtaa
    10681 ccagccagga agcccgcaat aataccgcca atgaagccag aaccggtgct gaccgccagc
    10741 ataccgccaa tcagacccgg cgtgagaccc ggacgatcgg caatggaaaa ggcaatataa
    10801 cctgccagta ccggcaccat caacgcaaag gctgaaccac caccaatctg catcagcgcc
    10861 gcagccaacg tacccggctc tttaaacgct tcgataccaa aagcaaaaga aagcgcgata
    10921 cacagaccac ctgcaacgac catcggcagc atgtaagaga cgcccgtcag caagtgacgg
    10981 taagcgcctg cactctcttt cttaccttca gtggtcgctg tttgagtttt gcccgccggt
    11041 tcatacggcg ttgcttcagc aaccgcttta tccagttcct gcgcggtttt cttcagcgcc
    11101 agaccggtag aggtgcgata catcggttta ccagcaaatt tcgccagatc cacttcaata
    11161 tctgccgcca caatcaccag atctgctgcg gctacttctt caggggtgat tgcattaccc
    11221 gcgccaaccg aaccacgggt ttcaactttc acccaccagc cacgtttttt cgcttcggtt
    11281 tcaatggctt cagccgccat aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca
    11341 actacgcgtt tcggaccgct ggcggcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa
    11401 ggtttcgcat ggcctttggc ttcactcagg aacagctcag ggtgcgcaac tgcccgggaa
    11461 atatcgccca gccagacatt tttaccgttc agcgcgctgt cgttcgggat ggaatcaccg
    11521 agaacaatcg ccatttcagc gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc
    11581 gccgccgcgc ccagcagggt cttcgccata taggcgcgtg cctgaccgag attagcgtca
    11641 ataatcagca gcgttttcat tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac
    11701 gcgcgccatc attgcggcca actgcggacg atcggtaata cccacattgc tttgacttac
    11761 cgccagggct gcaacagctg tcgccagacg cagtgtgtgt tcactggatt cacgcatcag
    11821 caagccataa atcaggccac caaccataga atcccctgcg ccaacggtgc ttacgacatc
    11881 gactgacggt ggtttggcga tccattcgcc ggaggcatta acccaaagcg cgccttcggc
    11941 acccagtgaa ataacgacat gcgcgatacc ttgttcacgt agcgcatgtg cagcttcaat
    12001 cacatctttc atttcaggca gtttacggcc tgcccagatt tccagctcgc ggcggttagg
    12061 tttcaccagc cacggtgccg ctttcaaacc tgctactaac gcttcacggc tactatcaaa
    12121 gataatgcaa ggacactgac tacgcaggcg agtcatccag tcggtgaacg cttccgggct
    12181 gacgcctgac ggtaagcttc cgctgacaca gaccatatcg aactgaccga gccagctcag
    12241 agaatcagtc acaaagcgtt cccagtcggc gggagtgact tcaaaacccg agaagttgaa
    12301 gtcggtcact tcgccgtctt tttccgtcag cttaacgtta attcgggtgc gcccctgtac
    12361 aacctggaaa cggttggcaa tgcccagctc gctgaacagt tgctgaaaac catcctgatt
    12421 gtctttaccg aggaagccgc caacagtgac atcaattccc aggtctttta acactttggc
    12481 cacgttgatg cctttacccg ccgcatgcag accggtggtt ttcaccaggt tcacttcgcc
    12541 gcgttcaatt tccgggcaga aaccaacaag gtcataagcc ggattaaggg tgatagtagc
    12601 aacacgtctg ctcattatgc gccctcccca agaccagcag cgatagcgtc gccaatggct
    12661 ttcagcgcct gttcagcatc tgcaccctgg gcggtaaagc gtaggcgatg acctttctta
    12721 acgccaagtg ccacaacttt catcagacta cgtccgtttg ccggtttgcc ggtgccatca
    12781 aggtttgtca cggtaatatc actgttaaat tgtttaatgg tattgaccag catggtacct
    12841 ggacgagcat gcaggccgtg ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg
    12901 gtcggcgcat catcgctggt cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag
    12961 cggtcagctt tattgtcgag taacaaatca gcgagacgct ttagaaccgc aatgggctga
    13021 tcgtcattca tcgccacact caccagcatg gctgccgttt cgccgtccac atcaaaagca
    13081 tttgccgcac ggcttaccgc aatcgcgcta cgcagattgc cttcggcgct atcgctcagc
    13141 cagataccct gtccgaggtt cagcggttgt tcattgatgg ctttggtgac gaaagtggcg
    13201 tcaactgccc ccgcctcttt cagacgagca gcgttcagcg cctgaagagt cagcagatcg
    13261 ctggcgacga tatccagcgt cagcatttcg ttgtcgagct tcagctgctc actctgcttt
    13321 tcgcccatca gcaatgcgcg aagttcttct gctgttgttg ctgacttcag ttgttcagca
    13381 acggaatcat cgctcagtac gtgggtcagc tggcgtagca ggcccaaatg ttcatccgag
    13441 ctggcagcaa taccgatcgc cacgtacgct acctggccgt caccccaggt gacgccttcc
    13501 gggaactgaa atacctgaac gccggttttc agcacctgat cgcgggtgtc ggtagtgccg
    13561 tgtggaatag caataccatt gccgaggaac gtagaggttt gctgttcgcg cgccagcatg
    13621 ccattgacgt agccttcagc tacattaccg gcctgcacca gcgccgcagc gacctggcga
    13681 atcgcctctt ctttgtctcc ggccttttcg cccggatgga tgtcctgtac ggataactgg
    13741 aacatagttc tcctctcttg ctgaattgaa acgattcagc ttctatgaga aaaaaagcgc
    13801 caacctggct tagggttaaa gacaagatcg cgctgaaacg tttcaagaaa gcataatact
    13861 tctgtttcag cacgcaagga aagccgtaaa gttatgagca aaagtttgat gtgctgcaca
    13921 attttttgtc atttttccta attgctgatg ggaaaatccg ttatcagcaa tttcatttca
    13981 gcagcgtcag cagccaatca gcaacagtga aatggcattt tgattattcg tggcgctttt
    14041 gatttcgctg tctgtttatt ttctgacaag cagcgtaaac tccgcgtctt cctcttccag
    14101 tgatcgacca gcatgcataa ctcccccgca gtctccagcg cgaaatcgtt tgacctgacc
    14161 tcgatggcgt ttttaatcgt tgcctttctc accggtattg cgggcgctct acaaaccccg
    14221 acactcagta tttttcttac tgatgaagta catgcccgcc cggcgatggt gggattcttc
    14281 tttaccggca gcgctgtcat tgggattctg gtaagtcagt ttctcgccgg gcgctctgat
    14341 aagcgcggcg atcgcaaatc gctgattgtc ttttgctgcc tgttaggcgt gctggcctgc
    14401 actctttttg cctggaatcg caactacttt gttttgctat tcgttggcgt ctttcttagc
    14461 agctttggct cgactgctaa cccgcaaatg tttgccctcg cccgtgaaca tgctgacaaa
    14521 accggacgtg aagcggtgat gttcagctct tttttacgcg ctcaggtttc actggcatgg
    14581 gtcattggcc cgccgctggc ttatgcctta gcgatgggtt tcagctttac ggtaatgtat
    14641 ctgagcgcag cggtagcgtt tattgtttgc ggtgtgatgg tgtggctgtt tttaccgtcg
    14701 atgcaaaaag aacttccact ggcgaccggc acggttgaag cgccgcgccg taaccgtcgc
    14761 gatacgctgc tgctgtttgt catttgtaca ttgatgtggg gctcgaacag cctgtacatc
    14821 atcaacatgc cgctatttat tatcaacgaa ctgcatcttc ccgagaaact ggccggtgtg
    14881 atgatgggga ccgccgccgg gctggaaatc ccgaccatgt tgattgccgg atatttcgcc
    14941 aaacgtttgg gtaagcgttt cttaatgcgc gttgctgccg tgggtggcgt ctgtttttac
    15001 gcaggaatgc tgatggcgca ttcacctgtc attctgttgg gcttgcagct gcttaatgct
    15061 atttttattg gcattctggg cggcatcggg atgctctatt ttcaggattt gatgcccggt
    15121 caggcaggtt cagccaccac gctctatacc aacacttcgc gcgtgggctg gatcatcgca
    15181 ggatcagtgg cgggcatcgt cgccgagatc tggaattatc acgctgtgtt ctggtttgcg
    15241 atggtgatga ttatcgccac tctgttttgc ttactgcgga ttaaagatgt ttaaggcgcg
    15301 gtcagcattt ccagatcgat aagccaggtc attgcctgct gacgcgagtt accgcacatt
    15361 tcagcgctgg cttgtaagcc cgcacaaact ttcggtcgca gtggcgaggt gaaaatttta
    15421 cagcgttgct gttcatcaag ctgaatgcag ggcgtattgg cgggcttgcc atctggcata
    15481 ccgggaatag ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc cggacggcat
    15541 tccatacgtt gttctcttaa ggttaaccac cggattgatg agcgcgcaca gtaccacctt
    15601 ttttgcacca gcaaaagtgc gaataccact tgcccgaaag gcccgtcgcg agtactttgt
    15661 cgcgatattt ttgacatttt cgactacagg aatttttcga tgccaagagc gaacgaaatt
    15721 aaaaaaggta tggtactgaa ttacaacggc aaactgctgc tggttaagga cattgatatt
    15781 cagtcgccca ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt
    15841 accgggctga aagtagaaga gcgtttcaaa ggagatgata tcgttgacac cgtgactctg
    15901 acccgccgtt acgttgactt ctcctatgtc gatggtaacg aatatgtctt tatggataaa
    15961 gaagactata ccccgtatac cttcaccaaa gatcagattg aagaagagtt gctgtttatg
    16021 ccagagggcg gcatgccgga catgcaggtg ctgacctggg acggtcaact gctggcgctt
    16081 gagctgccgc agaccgttga tctggaaatc gttgaaaccg cacctggcat caaaggggca
    16141 tctgccagcg cccgtaacaa accggcgaca ttgagcactg gtctggtgat tcaggtacca
    16201 gaatacttaa gcccgggcga aaaaattcgt atccatatcg aagaacgccg ttatatgggg
    16261 cgtgctgact aacttcagcc gcatgcagaa aagggatagc tcaggctgtc ccttttttaa
    16321 tttattatac caatcttcta ttttgcgctt cctgaacacc ttacgcccgc aatattcact
    16381 cgttgattga tcgccctcac acttcatcgc attaacaatc cagaccaatt tcaattgctg
    16441 tcatataact ttacactgtc attgttaatt aatcgttact aagacgtgac tcctatgaat
    16501 acaatcgcct ccgttacgct cccgcatcat gtacacgctc cacgctacga tcgccagcag
    16561 ttgcaatcac gtatcgttca ttttggcttt ggtgcctttc accgcgcaca tcaggcatta
    16621 ctgaccgatc gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga aatcagcttg
    16681 ttcagcggcg atcaactgat gagccagctc cgcgcacaga accatttata taccgtgctg
    16741 gagaaaaggg cggacggcaa tcaggcgata attgtcggtg ccgttcacga atgcctgaat
    16801 gcaaaactgg attccttagc ggcaattatt gagaagtttt gcgagccaca ggtggcaatt
    16861 gtttccctga cgattaccga aaaaggctat tgtattgacc cggccaccgg tgcactcgac
    16921 accagcaatc cgcggattat tcacgatcta caaaaccctg aagaacctca ctccgcgccg
    16981 ggtattctcg tcgaagcact gaaacgacgc cgtgaacgcg gccttgcacc gtttaccgta
    17041 ctctcctgcg acaatattcc cgacaatggc catgtggtga aaaacgcggt gctggggatg
    17101 gcggaaaaac gttcaccaga actcgccggg tggataaaag aacacgtcag ttttccggga
    17161 accatggtcg accgcattgt tccggctgca accgacgaat cactagcgga aatcagccag
    17221 catctggggg tgaatgatcc ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtg
    17281 gaagataact tcgtcgctgg gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat
    17341 gatgtcctgc catgggaaga gatgaaactg cgtatgctta atggcagcca ctcttttctc
    17401 gcttatctgg gttacctctc aggattcgcc catatcagtg attgcatgca ggatcgcgca
    17461 tttcgccatg ccgccagaac attaatgctg gatgagcaag cgccaacact gcgaattaaa
    17521 gatgtcgatt taacacaata tgcggataag ttaattgcac gttttgctaa cccggcgctg
    17581 aaacataaga cctggcaaat cgcaatggat ggtagccaga aattaccgca acgtatgctg
    17641 gcaggtattc gcatacatct ggcgcgcgaa acggactggt cgttgctggc attaggcgtt
    17701 gcaggctgga tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc
    17761 gatccgctta gcgataaaat tcgcgaactt gttgcgggca gtagcagtga acaacgcgtc
    17821 accgccctgc tttccctgcg tgaagttttc ggtgatgatc tgccagataa cccgcatttt
    17881 gtgcaggcta tcgaacaagc ctggcaacaa atcgcacaat tcggcgcgca tcaggcgcta
    17941 ttaaacaccc tcaaaattta acgatttctg cggttaaagc ggatgaagct cgccttcgtc
    18001 cgctcccccc ttctcttttc tgcctttttt agccaggatt aacgctcagt taacttttaa
    18061 gagtattgcg gagccgttgt gaccaggacc aacctcatca ccggttttct cggcagcggg
    18121 aaaactacgt cgattcttca tctgttagcc cataaagatc ccaacgaaag atgggcggta
    18181 ctggttaatg aatttgggga agtcggaatt gatggtgctt tgctcgccga tagcggcgca
    18241 ttgctgaaag agatccccgg cggctgcatg tgctgcgtta atggtttacc catgcaggta
    18301 ggattgaata ccttactgcg tcagggaaaa ccagaccgct tgttgataga gccgaccggg
    18361 ctgggccatc cgaaacaaat cctcgatctc ttaaccgcac cagtctatga accgtggata
    18421 gatctgcgcg ccacgttgtg cattctcgat ccacgcctgc tgctggacga aaaaagcgcc
    18481 agcaatgaaa acttccgtga ccagctggct gccgcagaca ttattgtcgc caataaatcc
    18541 gaccgtgcga cgcccgaaag tgagcaagcg ctacagcgct ggtggcagca aaatggtggc
    18601 gatcggcaat taattcacag cgagcatggg aaaattgacg gtcatcttct ggatttgccg
    18661 cgtcgcaatt tagccgagtt gcccgccagc gccgcacatt ctcatcatca tagcgtgaaa
    18721 aaagggttag cagcgttaag cctgccagag catcaacgct ggcgtcgcag tctgaacagc
    18781 ggacaagggc atcaggcttg tggctggata ttcgacgctg atacggtatt cgacaccatt
    18841 ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt gatgcgtatt
    18901 cccgaagggc tggtgcgaat caaccgtcag ggcgatgacc tgcacattga aacgcaaaac
    18961 gttgcgccac cggacagtcg tattgagctg atttccagca gcgaagctga ctggaatgcc
    19021 ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt
    19081 tcagtaagat aattagagaa aatatgatta aaaatttgcc gcaaatagtg ttgttgaata
    19141 ttgtcggcct cgcgctgttt ctttcctggt atattcccgt taatcatgga ttctggttgc
    19201 cgattgatgc ggatattttt tatttcttta atcagaaact ggtcgaaagt aaggcctttt
    19261 tgtggctggt tgcattgacc aacaatcgcg ctttcgacgg ttgttctctg ctggcgatgg
    19321 gaatgttgat gctgagtttc tggctgaaag aaaacgcccc tggcagacga cgtatcgtga
    19381 ttattggtct ggtcatgcta ttaactgcag tggtattaaa ccagctgggt caggcattaa
    19441 ttcctgtaaa acgggccagc ccaacattga cttttaccaa tattaaccgc gtcagcgaac
    19501 tgctctctgt ccccacgaaa gatgcctcac gagatagctt tcccggtgat cacggtatga
    19561 tgctgcttat tttttcggca ttcatgtggc gttatttcgg caaagttgca ggccttatcg
    19621 cccttattat ttttgtggtt tttgcatttc ccagagtaat gattggcgca cactggttta
    19681 ctgacatcat tgtaggttcg atgaccgtga tattgatcgg tttgccctgg gtgttgctga
    19741 cgccattaag tgatcgatta atcacctttt ttgacaaatc actaccggga aaaaacaaac
    19801 atttccaaaa caaataactc acagtaatta acatcatcag ggttattttt atagtgagga
    19861 taatcctgat gatgcgcacc gtgctttcat ctatcgaacg caaaaatcat tctctaagta
    19921 aatgaatgga ttgcatgcgc ttcactcaat cgtactttaa ttgaccaatc ccgcttatta
    19981 actttctgta tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc
    20041 aatcgggtaa tctcgaactc gttttgcctc ggcggtagat tatcctcaca gcatataatt
    20101 ttgtgcgtta gtccacagat ttggccttaa ggaattgttt caacatgccc aggtaattag
    20161 tctcgtgtcg cttggcattt ttttataacg atatttgtcg ttaaggactt caagggaaaa
    20221 caaacaacat ggtcaaatct caaccgattt tgagatatat cttgcgcggg attcccgcga
    20281 ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa caccgcaaag aatatgcatc
    20341 ctgagacacg tgcagtgggt agtgaaacat catcactgca agcttctcag gatgaatttg
    20401 aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat gctgactgga
    20461 aaggcgtacg ttatcgcctg ggcggcagca ctaaaaaagg tatcgattgt tctggtttcg
    20521 tacagcgtac attccgtgag caatttggct tagaacttcc gcgttcgact tacgaacagc
    20581 aggaaatggg taaatctgtt tcccgcagta atttgcgtac gggtgattta gttctgttcc
    20641 gtgccggttc aacgggacgc catgtcggta tttatatcgg caacaatcag tttgtccatg
    20701 cttccaccag cagtggtgtt attatttcca gcatgaatga accgtactgg aagaagcgtt
    20761 acaacgaagc acgccgggtt ctcagccgca gctaataaac cgtttggatg caatcccttg
    20821 gctatcctga cgagttaact gaaagcactg cttaggcagt gcttttttgt tttcattcat
    20881 cagagaaaat gatgtttccg cgtcttgatc caggctatag tccggtcatt gttatctttt
    20941 aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt catacgcgct cccaattctg
    21001 gacgtaagct cctgcttacc tgcattgttg caggcgtgat gattgcgata ctggtgagct
    21061 gccttcagtt tttagtggcc tggcataagc acgaagtcaa atacgacaca ctgattaccg
    21121 acgtacaaaa atatctcgat acctattttg ccgacctgaa atccactact gaccggctcc
    21181 agccgctgac cttagatacc tgccagcagg ctaaccccga actgactgcc cgcgcggcat
    21241 ttagcatgaa tgtccgtacg tttgtgctgg taaaagataa aaaaacattc tgttcatctg
    21301 cgactggtga gatggacatt ccactcaatg aattgattcc ggcgctcgac attaataaaa
    21361 acgtcgatat ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca
    21421 tctggtatcg caaccctttg ctgaaaaata gcggcgtgtt tgccgctctg aatctcaatc
    21481 tgacgccttc tctcttttat agttcacggc aggaagatta cgatggcgtc gccctcatta
    21541 ttggcaatac tgcgctatct accttttctt cacgtttgat gaacgttaat gaattaaccg
    21601 acatgccggt ccgtgaaact aaaattgcgg gcattcctct gaccgttcgg ctttatgcag
    21661 atgactggac atggaacgat gtgtggtacg catttttact gggcggcatg agtggaactg
    21721 tcgttggcct gctctgctat tacctgatga gcgtacgtat gcgccccggc agagaaatca
    21781 tgaccgccat caagcgcgaa caattttacg tggcgtatca accggtggtg gatacacaat
    21841 ctttgcgagt aacgggcctg gaagtactgc tacgctggcg gcatcctgtc gcgggagaaa
    21901 tacccccgga tgccttcatt aactttgccg aatcgcaaaa gatgattgtg ccactgactc
    21961 agcacctgtt tgagttgatt gcccgcgatg ccgcagaatt agaaaaagta ctgccggtag
    22021 gcgtcaaatt tggcattaac attgcgccgg accatctgca cagcgaaagc tttaaagcag
    22081 atatccagaa actgctcact tccctgcccg cacaccattt ccagattgtg ctggaaatta
    22141 ccgagcgcga tatgctgaaa gagcaagaag ccacgcaact ctttgcctgg ctacactcgg
    22201 tcggcgtaga aattgctatt gatgacttcg gtaccgggca cagcgcgctt atctaccttg
    22261 agcgttttac gctcgattat ctgaaaattg accgtggatt tatcaacgcc atcggtacgg
    22321 aaacgatcac ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgt ctcaatatgc
    22381 tgacggttgc tgaaggggtc gaaacgccgg aacaggcgcg atggctaagc gaacgcggcg
    22441 ttaatttcat gcaaggctac tggattagtc gcccgttacc gctggacgat tttgtccgct
    22501 ggctgaagaa accgtatacg ccgcagtggt aaggtgtgct tacgtccctt attattcata
    22561 gtgaaagcat gccggattgc ggctaatgat gagtaaaagg aaatccgttg cagatgattg
    22621 tgcgcatact gctgctgttt atcgctctgt tcacctttgg tgcgcaggcg caggctatca
    22681 aggaaagcta tgcctttgcc gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt
    22741 ttgattatgt gaaccccgcc gcgccaaaag gggggcagat aacgttgtca gccctcggca
    22801 ccttcgataa tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc accgagcagc
    22861 tgtacgacac gctatttaca acttccgatg acgaaccagg cagttattac ccgctgattg
    22921 ctgaaagtgc acgctatgct gacgattatt cctgggtgga ggtcgctatt aatccgctcg
    22981 cccgttttca tgatggttcg cccattactg cccgcgatgt agagtttact tttcaaaaat
    23041 ttatgaccga aggcgtgccg caatttcgtc tggtctacaa aggcaccacc gtcaaagcca
    23101 ttgcgccgtt aaccgtgcgc attgagttag ctaaacccgg caaagaagat atgctgagtc
    23161 tgttttcgct gccggtgttt ccagaaaagt actggaagaa tcacaaactt agcgacccgc
    23221 tcgccacgcc tccgcttgcc agtggtccgt accgaattac gtcctggaaa atggggcaaa
    23281 atattgtcta ttcccgcgtg aaagattact gggcagcaaa cttaccggta aaccgtggac
    23341 gctggaattt cgacaccatt cgctacgatt attacctcga tgataatgtc gcctttgaag
    23401 cgtttaaagc cggtgccttt gatttgcgta tggaaaacga cgccaaaaac tgggccacgc
    23461 gctataccgg taaaaatttc gataagaaat acatcatcaa agatgagcaa aagaacgaat
    23521 cagcccagga tacgcgctgg ctggcgttta atatccaacg tccggtattc agcgatcgcc
    23581 gggtgcggga agcgatcact ctcgcctttg actttgaatg gatgaacaaa gcgttgtttt
    23641 acaatgcctg gagtcgcacg aacagttatt ttcagaatac cgaatacgcg gccagaaatt
    23701 accccgacgc cgcggagctg gtgcttctgg caccaatgaa aaaagatcta ccgccagaag
    23761 tcttcacgca aatctaccag ccgccggtat ccaaaggcga tggctacgat cgtgacaacc
    23821 tgttaaaagc cgacaaactt ctcaacgaag cgggctgggt gctgaagggt cagcaacgcg
    23881 taaatgccac aacgggtcag ccgctcagct ttgagttatt gctaccttcg agcagcaata
    23941 gtcagtgggt attgccgttc cagcacagcc tgcaacggct gggtatcaac atggacattc
    24001 gcaaagtgga taactcgcaa atcaccaacc gcatgcgcag tcgcgactat gacatgatgc
    24061 cgcgcgtatg gcgggcgatg ccgtggccca gttccgattt acagatttcc tggtcatcgg
    24121 aatatatcaa ttccacttat aatgcccccg gcgtgcaaag cccggttatc gactcgctga
    24181 tcaaccaaat tattgccgcg cagggaaata aagaaaaatt actgccgttg gggcgagcac
    24241 tggatcgcgt attaacgtgg aattattaca tgctgccaat gtggtacatg gcggaagacc
    24301 gtctcgcctg gtgggataaa ttctcccaac ccgctgtacg ccctgtttac agcctgggta
    24361 tcgatacctg gtggtatgac gttaataaag cggccaaact gccgtcagcc agacaacagg
    24421 gagagtagat gggcgcttat ctgattcgcc gtctgttgct ggtgatccca acgttatggg
    24481 cgattatcac tatcaacttt ttcatcgtgc aaattgcgcc tggcggtccg gtcgaccagg
    24541 ccatcgccgc cattgagttt ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg
    24601 ttcgcgccag ccatgcgcaa acgggcgtcg gcaatatcag cgacagtaat taccgtggcg
    24661 gacgcggatt agatccagaa gtgatcgccg agatcactca tcgctacggt ttcgataagc
    24721 cgatccacga acgttacttc aaaatgctct gggactacat ccgctttgat tttggcgaca
    24781 gcctttttcg cagcgcctcg gtactgacgc tgattaaaga tagtctgccg gtttccatca
    24841 ccctcggatt atggagcacg ctgattatct atctggtgtc gattccgtta ggcattcgca
    24901 aagctgttta taatggtagt cgctttgacg tctggagtag tgcatttatc atcatcggct
    24961 acgccattcc ggcctttttg tttgccatcc tgctgattgt cttcttcgca ggcggcagct
    25021 atttcgacct gttccctcta cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt
    25081 atcagaaaat caccgattat ctgtggcata tcacgctgcc ggtgctggcg acagtgattg
    25141 gcggctttgc ggcgctgacc atgctgacaa aaaactcatt ccttgatgaa gtgcgcaagc
    25201 aatacgtggt gaccgcccgt gcgaaagggg taagtgaaaa aaatattctc tggaaacatg
    25261 tgttccgcaa cgccatgctg ctggtgattg ccggttttcc ggcgacgttt atcagcatgt
    25321 tttttaccgg ctcgctgctg attgaggtga tgttttcact caatggtctt ggcttactgg
    25381 gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg tatattttca
    25441 ccctgattgg cctgctgctg aatattgtca gtgatatcag ctatacgctg gtcgatccgc
    25501 gtatagattt tgagggacgt taatgtcgcg actcagcccc gtcaatcagg cccgttgggc
    25561 gcgttttcgt cataaccgtc gcggctactg gtcgttatgg attttcctcg ttttgtttgg
    25621 tttgagtttg tgttctgaac tgatcgccaa cgataaaccg ttgctggtac gttatgacgg
    25681 cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg gcccgctggc
    25741 aagccaggct gattatcagg acccgtggct gaaacagcgg ttggaagata aaggctgggt
    25801 actatgggca ccgattcgct ttggcgctac cagtatcaac ttttctaccg acaagccctt
    25861 cccctcccca ccctcccggc aaaactggct gggaacggat gccaatggcg gcgatgtgct
    25921 ggcacgtatt ctctatggca cgcggatctc agttctgttt ggcctgatgc tgaccctctg
    25981 ctccagcgtg atgggcgtgc tggcgggggc gctacaaggc tattacggcg gtaaagtcga
    26041 tctctggggg caacgtttta ttgaagtatg gtcggggatg ccgacgctgt ttttgattat
    26101 tttactttcc agcgtcgtac agcctaactt ctggtggctg ttggcaatta ccgtcttgtt
    26161 tggctggatg agtctggtcg gcgtggtgcg ggcggagttt ttacgtaccc gtaatttcga
    26221 ctacatccgc gcggcgcagg cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat
    26281 gttgccaaat gccatggtcg ctaccctcac ctttttaccg tttattttat gtagttcgat
    26341 caccaccctg acctcactcg atttcctcgg cttcggtctg ccgctcggtt caccgtcact
    26401 cggcgaactg ctgttacaag ggaaaaataa ccttcaggcc ccgtggcttg ggatcaccgc
    26461 cttcttgtcg gtggcgatat tgttgtcttt gctgatcttt attggtgaag ccgtccgcga
    26521 cgcatttgat cctaataagg cggtgtagca tgacgcaaac tctgttagcg attgaaaatt
    26581 tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt agtcaatgat gtttcacttc
    26641 agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta
    26701 ccgcgctgtc aattttacgc ctgctccctt ccccgccggt tgaatatctc tccggcgata
    26761 ttcgttttca tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg
    26821 gtaataagat cgccatgatt tttcaggagc cgatggtgtc attaaatcca ttgcataccc
    26881 tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc
    26941 gtggcgaaat tcttaactgc cttgatcgcg tcggtatccg ccaggcggca aaacggctga
    27001 cagattatcc gcatcagctc tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc
    27061 tgttaacgcg acctgaatta ttaattgccg atgaaccaac caccgcactg gacgtctctg
    27121 ttcaggcgca aattttacag ctgttgcgcg aactgcaagg cgagctgaat atgggtatgc
    27181 tgtttattac tcataacctc agcattgtca gaaaactggc ccatcgcgtg gcggtaatgc
    27241 aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct atttgcctca cccactcatc
    27301 cttacacaca aaagctactc aacagtgaac cgtcaggcga cccggtacca ttgccagaac
    27361 ctgcctcaac gttgctggat gttgaacagc ttcaggttgc cttccccatt cgcaaaggga
    27421 ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa catcagtttt acgctacgag
    27481 cgggtgaaac actgggttta gtaggcgagt caggttccgg gaaaagcacg acgggactgg
    27541 cgctgctgcg actgattaac tctcagggca gcattatctt tgacggtcag ccactgcaaa
    27601 atttaaatcg tcgccagctg ttacctattc gtcatcgcat tcaggtggta tttcaggatc
    27661 caaactcctc gctcaaccca cgactcaacg ttttgcagat tattgaggaa ggcttacggg
    27721 ttcaccagcc gacgctttct gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg
    27781 aagtgggatt agatcctgaa acacgccacc gttatccggc ggagttctct ggtggtcagc
    27841 ggcaacgtat tgcgattgcc agggcgttaa ttcttaagcc ctcgctgatc attcttgatg
    27901 aaccaacatc atcgctcgac aaaacggtac aggcgcaaat attgacgcta ttgaaatcat
    27961 tgcaacaaaa gcatcaactg gcctatttgt ttatcagtca cgatttgcac gttgtccgcg
    28021 cgttatgtca tcaggttatc gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg
    28081 cgcgcgtgtt tgccgcaccg cagcaggagt atacgcgtca gctactggcg ttgagctgac
    28141 gcttaaaaag gattgtagtt tgagaagggt tcggcaattg ccacaccaaa atttttcaat
    28201 cggcatgttg cagcaaactc gtcctggcga tttacaaaca ggcacggctc accttcacac
    28261 tccagtaccg aacatggaac ttcgatgtcg cttaacgcct ggctgagttt gtacatcacc
    28321 gaccatgcat tatcaccatc cgggctaagc aatttaagcg ccaccaggct gttgtcaccg
    28381 cacggtccat tttgcggaat cggttcaacc ttcgaacctg cgaaaccagc agaccagcga
    28441 tagttctgcg gcagtcgatg gacgattgag agttgtaatg aagtcactta ttttccccgg
    28501 aagcacattt acttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct
    28561 ttatacagat cgtaaagatt gatgcctcgc cagcgtggcc tctatggctc tgatttaaat
    28621 aatatctgcg ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact
    28681 caaccttttt aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac
    28741 atatatcggg ggaatgatgg atttgtggtt gacatgcatc aacaaaggaa gccttttagc
    28801 ttcctcgttg tgcaatagat caccgttttt tcggccgact ggcgtacaga cagaagagaa
    28861 tggagctggt tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg
    28921 caagagaaag taatgcgcca acaattgccc ctatgccgaa acggaaggtt cctgccagcg
    28981 aagatgccgt tcccgccata tggggaaact catcaagaat gaccgccatc gcattagatg
    29041 acaccatcga cacgcagccc acaaacgccg caacgccaac caccagcgac cagaatccca
    29101 gccccagcag cgcactgatg accatccacg ccgccataat aaactgtatc cacaaccccg
    29161 agcggaacat atttaacgcg ccaattcggc ggacgaagcg gctgttaaag atagtcatca
    29221 cgaacaggaa aacaatgttt agcgcaaagt aataaccaaa gttttccggc gcgacgtggt
    29281 taatttcaat gtagacaaac ggtccggcgc ttaagaacga gaacatcccg gcaaagctga
    29341 aaccactggc aagcatataa ctcaggacac gcttatgacg gaacagcgcc gcaaagttac
    29401 caatagtggt acgaatgtga aatggctgac gacgctctgg cggcaacgtt tctttaatca
    29461 ggaagaaaat cattgccgaa gccagaatcg ccgctaatgc caggatccag aagatgtaat
    29521 gccagctcag ccacaccagc acccaaccgc cgacaatcgg tgccatcaac ggtgcaatag
    29581 tggtcaccag catgacaaac gacatcatcc gcgagaactc ttctttcggg taaatatcgc
    29641 gcatcagggc attaatgacc acgctggccg cagccgcagc cagcccgtgg aagaaacgca
    29701 tcacaatcag ctgatcgatg gtgtttgcca acgcacacgc caccgcggcg gcggcaaaca
    29761 ccagcgtacc gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga
    29821 ttaactgccc caacgcaaag cccagaatat aagtactgag ggtcatctgc gtactgcctg
    29881 ccggtacgcc aaactgcgct gaaattaccg gtagcgcggg cagatacata tcaatcgaca
    29941 gcggcatcaa catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc
    30001 gggtggtcac aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt
    30061 cttcagttaa cggacgatat tcaccgggag ctaaatcagc atccagcgta atgccgccaa
    30121 tacgttcacg atgcagctca accacatggt tacccacggc ggcgaacata cgtttcacct
    30181 gatgataacg cccttcgctg atggtcaaac gaacctgcgt tggggtaatc acttccagca
    30241 ccgccggctt agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg
    30301 ccgtatcgtc agctacgggt gattccagcg tcaccagata ggtcttctcg caatgatggc
    30361 gcggagaagt aatgcggtgc gaccactgac catcatctgt cattagtacc agaccggtgg
    30421 tatcgatatc cagacgccct gcagcgtgca gtttccacgc taccggttca tcaagaaaat
    30481 agagcaccgt tgggtgatcc gggtcgtccg tggagcaaac atagccctga ggtttattga
    30541 gcatgaagta acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat
    30601 gttcagggag cagtttgaac gctgcattac ggacgatttc gccatcgacg gtgacacgat
    30661 tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt
    30721 tatcaagtcg catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa
    30781 aatctgaact gttgtctgcc cagtatagcg gtctaataac ttccctcaag ggaaaaagat
    30841 ccgtggcata ctattagcag aataatctac ctacgcgaga ccatgatttt tacactccgc
    30901 ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct
    30961 gccgttatcg tgctgcccac cggtgcaggt aaaagcctgg tgatagcgga actggcgcgg
    31021 ctggcgcgtg gtcgcgtgct ggtgctggca cacgttaaag aactggtggc gcaaaaccat
    31081 gcaaaatatc aggcgctggg gctggaagcc gatatttttg ccgccgggct aaagcgcaaa
    31141 gagagccacg gtaaagtggt atttggcagc gtgcagtcgg tcgcccgtaa tcttgatgcc
    31201 tttcagggtg aattttcgct gttgattgtc gatgaatgtc accgtattgg tgacgatgaa
    31261 gagagccagt atcagcaaat cctcactcac ctgacaaaag tgaatcccca cttacgcctg
    31321 ctggggctga ctgccacgcc ttttcgattg ggcaaaggct ggatctacca gttccattat
    31381 cacggcatgg tacgcggcga tgagaaagcc cttttccgtg actgcattta tgagctgccg
    31441 ctgcgttata tgattaaaca cggctatctg acgccgccag aacgactgga tatgccagta
    31501 gtgcaatacg atttcagccg cttgcaggca cagagtaacg ggcttttcag cgaagccgat
    31561 ctcaaccgcg agctgaaaaa acagcaacgt attaccccgc acatcatcag ccagattatg
    31621 gagttcgccg aaaagcgtaa aggagtgatg attttcgccg ccaccgttga acacgccaag
    31681 gagatagtgg gactgctgcc tgccgaagat gcagcactga ttactggcga cacacccggc
    31741 gctgagcgcg atgtgttaat tgaagatttt aaagcccagc gttttcgcta tctggtcaac
    31801 gtcgcggtac tgaccactgg atttgacgcc ccgcacgtcg atcttattgc tattctgcgc
    31861 cctaccgaat cggtgagtct ttaccaacaa attgtcgggc gcggtctgcg tctcgctcca
    31921 ggcaagactg attgcttaat tcttgattat gcgggtaacc ctcacgatct ctacgcgccg
    31981 gaagttggta caccgaaagg caaaagtgac aacgttccgg tacaggtttt ctgccctgcc
    32041 tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt gattgaacac
    32101 tttggtcgcc gctgtcaggg atggtttgaa gatgacgacg gtcatcgaga acagtgtgat
    32161 ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg aaaacgatat tgccgcccgc
    32221 cgctgccgcg aatgtgacac cgtactggtt gatccggatg atatgttaaa agcggcgcta
    32281 cgactgaaag acgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac
    32341 gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggcgcgga tgtgagtgag
    32401 cgtttccgtc tgcaaacgcc cgctcagcgt accgccttcg agcagctttt tatccgcccg
    32461 catacgcgca caccgggcat ccctctgcgc tggatcaccg ccgctgatat cctcgcccag
    32521 caagcattac tgcgacaccc ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa
    32581 gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt
    32641 taatggtagt tttgattgat gtataaggcg attgagtata gaatctcgcc cgcttttgca
    32701 tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga
    32761 gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg
    32821 ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcgccgct
    32881 ggctgtcgaa ctggatcacg acaaagtcat gaacatgcaa gttaaagctg aattctatag
    32941 cgaagttctg accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca
    33001 gcgtcacccg tacaaaccga agctgctgca catcgacttc gttcgcgctt aa
//
LOCUS       DAOTRK010000043        32853 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.043, whole genome shotgun sequence.
ACCESSION   DAOTRK010000043 DAOTRK010000000
VERSION     DAOTRK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.043"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(195..1787)
                     /gene="lsrK"
                     /locus_tag="QIE41_001929"
     CDS             complement(195..1787)
                     /gene="lsrK"
                     /locus_tag="QIE41_001929"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDS3530821.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNND
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1866..2819)
                     /gene="lsrR"
                     /locus_tag="QIE41_001930"
     CDS             complement(1866..2819)
                     /gene="lsrR"
                     /locus_tag="QIE41_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDS3530822.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIASAMKGGYINALVTDQDTAAAILRS"
     gene            3068..4603
                     /gene="lsrA"
                     /locus_tag="QIE41_001931"
     CDS             3068..4603
                     /gene="lsrA"
                     /locus_tag="QIE41_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDS3530823.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTIELSTDDIIQAITPALREKSLSASQKLWLELPGNLAQHAAGTSMLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4597..5625
                     /gene="lsrC"
                     /locus_tag="QIE41_001932"
     CDS             4597..5625
                     /gene="lsrC"
                     /locus_tag="QIE41_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDS3530824.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILMAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            5625..6617
                     /gene="lsrD"
                     /locus_tag="QIE41_001933"
     CDS             5625..6617
                     /gene="lsrD"
                     /locus_tag="QIE41_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDS3530825.1"
                     /translation="MRIRYGWELALAALLVMEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6629..7651
                     /gene="lsrB"
                     /locus_tag="QIE41_001934"
     CDS             6629..7651
                     /gene="lsrB"
                     /locus_tag="QIE41_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDS3530826.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADELLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7678..8553
                     /gene="lsrF"
                     /locus_tag="QIE41_001935"
     CDS             7678..8553
                     /gene="lsrF"
                     /locus_tag="QIE41_001935"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDS3530827.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8577..8867
                     /gene="lsrG"
                     /locus_tag="QIE41_001936"
     CDS             8577..8867
                     /gene="lsrG"
                     /locus_tag="QIE41_001936"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDS3530828.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFFIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8924..9682
                     /gene="tam"
                     /locus_tag="QIE41_001937"
     CDS             8924..9682
                     /gene="tam"
                     /locus_tag="QIE41_001937"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDS3530829.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGVDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9686..10600)
                     /locus_tag="QIE41_001938"
     CDS             complement(9686..10600)
                     /locus_tag="QIE41_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDS3530830.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10807..12258)
                     /gene="uxaB"
                     /locus_tag="QIE41_001939"
     CDS             complement(10807..12258)
                     /gene="uxaB"
                     /locus_tag="QIE41_001939"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDS3530831.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12485..13903)
                     /locus_tag="QIE41_001940"
     CDS             complement(12485..13903)
                     /locus_tag="QIE41_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDS3530832.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHKCGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14042..14401)
                     /locus_tag="QIE41_001941"
     CDS             complement(14042..14401)
                     /locus_tag="QIE41_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDS3530833.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLDKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRCCLAKWHNIPQGVSLSEQQQR
                     YIVAVIYHWLVIQMNQP"
     gene            complement(14401..15327)
                     /gene="glsB"
                     /locus_tag="QIE41_001942"
     CDS             complement(14401..15327)
                     /gene="glsB"
                     /locus_tag="QIE41_001942"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDS3530834.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15391..16779)
                     /gene="sad"
                     /locus_tag="QIE41_001943"
     CDS             complement(15391..16779)
                     /gene="sad"
                     /locus_tag="QIE41_001943"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDS3530835.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITSEMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGAHLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16880..17761
                     /locus_tag="QIE41_001944"
     CDS             16880..17761
                     /locus_tag="QIE41_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3530836.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTLPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17839..18954
                     /gene="yneK"
                     /locus_tag="QIE41_001945"
     CDS             17839..18954
                     /gene="yneK"
                     /locus_tag="QIE41_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDS3530837.1"
                     /translation="MVTPVSISNYISLPDNFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTFLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLHPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFGNKEPNLLTAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQD
                     FPHFQDLFLFFANMLLHYHQFMNPNESDLIDVLMPASLSDDKIIKHMARKTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            19104..20294
                     /gene="ydeA"
                     /locus_tag="QIE41_001946"
     CDS             19104..20294
                     /gene="ydeA"
                     /locus_tag="QIE41_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDS3530838.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGAFITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20319..20984)
                     /gene="marC"
                     /locus_tag="QIE41_001947"
     CDS             complement(20319..20984)
                     /gene="marC"
                     /locus_tag="QIE41_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDS3530839.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21196..21630
                     /gene="marR"
                     /locus_tag="QIE41_001948"
     CDS             21196..21630
                     /gene="marR"
                     /locus_tag="QIE41_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDS3530840.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKRVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21651..22034
                     /gene="marA"
                     /locus_tag="QIE41_001949"
     CDS             21651..22034
                     /gene="marA"
                     /locus_tag="QIE41_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDS3530841.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22066..22284
                     /gene="marB"
                     /locus_tag="QIE41_001950"
     CDS             22066..22284
                     /gene="marB"
                     /locus_tag="QIE41_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDS3530842.1"
                     /translation="MKPLLSAIAAAFILFSTQGIAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22341..23780)
                     /locus_tag="QIE41_001951"
     CDS             complement(22341..23780)
                     /locus_tag="QIE41_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDS3530843.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLKFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLNYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23805..25478)
                     /locus_tag="QIE41_001952"
     CDS             complement(23805..25478)
                     /locus_tag="QIE41_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDS3530844.1"
                     /translation="MNIKTINVSLLSFSIITALFPLNALATKLTIEQRLELLENELSQ
                     NKQELRATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVVRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25534..25845)
                     /locus_tag="QIE41_001953"
     CDS             complement(25534..25845)
                     /locus_tag="QIE41_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDS3530845.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ELACLQAHKIQTALISQDEGCGKIEVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25873..27195)
                     /locus_tag="QIE41_001954"
     CDS             complement(25873..27195)
                     /locus_tag="QIE41_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDS3530846.1"
                     /translation="MGLMTSFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNRVFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGVFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVSRSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFILVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(27309..27620)
                     /locus_tag="QIE41_001955"
     CDS             complement(27309..27620)
                     /locus_tag="QIE41_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDS3530847.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27819..28517
                     /locus_tag="QIE41_001956"
     CDS             27819..28517
                     /locus_tag="QIE41_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDS3530848.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPPIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVAHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(28562..29461)
                     /gene="eamA"
                     /locus_tag="QIE41_001957"
     CDS             complement(28562..29461)
                     /gene="eamA"
                     /locus_tag="QIE41_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDS3530849.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29656..30843
                     /gene="ydeE"
                     /locus_tag="QIE41_001958"
     CDS             29656..30843
                     /gene="ydeE"
                     /locus_tag="QIE41_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDS3530850.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETVSVWTPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            30970..31065
                     /gene="mgtS"
                     /locus_tag="QIE41_001959"
     CDS             30970..31065
                     /gene="mgtS"
                     /locus_tag="QIE41_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDS3530851.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(31263..32174)
                     /gene="dgcZ"
                     /locus_tag="QIE41_001960"
     CDS             complement(31263..32174)
                     /gene="dgcZ"
                     /locus_tag="QIE41_001960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDS3530852.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGIRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRVSAPFNIH"
     gene            complement(32429..32821)
                     /gene="ydeI"
                     /locus_tag="QIE41_001961"
     CDS             complement(32429..32821)
                     /gene="ydeI"
                     /locus_tag="QIE41_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDS3530853.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKKMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         8581 a         8125 c         7616 g         8531 t
ORIGIN      
        1 atattcgcta taaaataagg gttatgctta atattttaag aaaaatagca atttcccctt
       61 gaatatatta ctggcgatgg aacgatgaat ctgcaagctg gcatttaaca aaccagctct
      121 aaaagaaggg aaatgagaat accaatactc aaaaactaac agccacggtc atcatgatgt
      181 ggctgtcaat gaaactataa cccaggcgct ttccataacg acgtcgtcag cccgtgatca
      241 accagcccca gctgatcctg ataaactgcc tgccacttat cgcgtgaatc ctgataaagt
      301 tcatgctttt ccgggtctgg tgtgtgcgtc cgttcccagc gaaccaggcg ttctccggtt
      361 tctgccattg atgaaaaaat tccggcaccg acgccagctg caatggcaca tcctaatgca
      421 gtggcttctt tgaccaccgg aatattgacg ggtaatcccg agacatcagc gagaatttga
      481 ctccataatt tcccttttga acctccgcct gcaaagacta acgatgaagg atgaatattc
      541 gagaaatcag cgatttgctg caagttacac gctgatacta tcgccgcatt ttcttccagc
      601 gcacggaaca atgtcgcttt gttacattta tccggatcaa tagacaaatt aataaaggaa
      661 ggcgcagcgt gataccaggt tttaaagcgc attctgtcgg agaatatcgg cattacgccc
      721 cacgacccag gcggcacccg acttgccatc tcttcaagca gcgtataggt gtcgatgcct
      781 aaacgttcgg caatcagttt ttcttcggca cagaaagcat cgcggaacca gcgcatggtg
      841 agtccggtaa aaaagcttat agattcagct tgtaccatgc caggaataac atgaggatta
      901 acgcgcacgt tcatttctgg gtctgtcacc ggcgcggcta agtttacaac ttgctgccag
      961 aatgtgccgc caagaacagc ggtttgtgcc ggacggacaa cgcctaaccc aaggcaacca
     1021 agttgcacgt cgcctcctcc aacgaccacc ggagtgcccg ccttcagacc gcagagttcc
     1081 gccgcttgtg aacttaccac gcccagcaat gtgccggttt ctttgacagg agaaagaata
     1141 tcggcccgca gtccggccat atccagcaat gcaggtttcc agtcacgggt ggttagatca
     1201 agaagtcccg tggtgccagc gttagaggga tccaccgcca gttcgccgct gagcatatag
     1261 gccagccagt cgctgatcat ggtgatggtc gacgcctgtc ggtagatatc agaacgatgg
     1321 tgcgccagcc aaagtaatct gggaatagcg cttaaagcca gcgtttgtcc ggtggcgcga
     1381 taaacttcgt tttcaaaggt attgttgtgc agttctttaa gttcgctaac ttcgcgcgcc
     1441 gctctggcat ccacattggc gcaggcccag atcggtgttc catcattgtt atataaaaca
     1501 atgccttcac gcatcgaaca tgccgaaacg gcagcgatat actccggggc tatgccggcg
     1561 ttgtgcagcg cctggcgcat acactcacac gccagttgcc agtttttgtt gagatcaaat
     1621 tccatagaac caggaacgtc cggtactgcc aggtgccgcc actccgcctg tcccacggct
     1681 atttgattgc cttccaggtc gaatatcaca gcccgaatac ttccggtacc tgcatccagc
     1741 gccatcaggt aatactttga ttctgaaggg gtaaagagtc gagccattag attatcctcg
     1801 gcttatgcgt tctgtctgtt cctctgtacg ttctccatca ttcccggtaa taaggtcatg
     1861 caaatttaac tacgtaaaat cgccgctgct gtgtcctgat cggtaaccag tgcgttgata
     1921 taaccgcctt tcattgcaga ggcaatggct tcggctttat tttctccccc tgctacgcca
     1981 acccggacgg gaatggtctt cagcgcgctt aaaggtaagc caatcagttc gttatgtatt
     2041 ttgatatccg tgacaacgtc accttttgca tcaaaaaagt agcctaagat gtcgccaacc
     2101 gccccttttc ggccaatcat taactgttcg ccctggctga tataaccgga gcgaatgatc
     2161 gtcgcgtcgt cttgttgact cacggcccca atgccgacaa tcgccacatc cgctgcttgc
     2221 gcggctaaca gaacatcttt gacgcaattt tcatttttta gcgtacgggc aatgtcagcg
     2281 gaggatgccc gcagcggagc cggaataata ttcacactgc acgccgcgtt aagctgtccg
     2341 atacccgtca tataagaacc gacgccaccg gagagcgtga ccaggcgaat ttgctgcgac
     2401 gaaataaaac cacttaagcg ttgcagcgta ttcatggttg cctcgccaaa accaatcgcc
     2461 agcatctgtt gtggttgaag taaactcatc aacatatgcg ccgcgcctat ccctagtcgc
     2521 ccgccgacat cagcatccgc aagccctggg atcacccgga catgttgcag cgaaaactga
     2581 cgacgtaatt gtgtttcata ttccagacag ccttcaaagc gagaattaat ctgtacgcga
     2641 ataatgccgg attgatgccc tttctccagt aatcgcgaca ctttcaaacg ggtcaggccg
     2701 agacgatcgc tgatctcact ctgggtcagc ccgtcgtggt agtaaaacca cgcaatccgc
     2761 gcgacctgtt cttcttcaca cattccctgt tctgaaatta ccgaatcgtt gattgtcata
     2821 attcattctt cactttgaac atacttaaat ctttaatgca attgttcagt tattgcgcat
     2881 ttatatctgt gatggcaacc gcagtttgac tctacgagca tgaacaaacg caaccgtgaa
     2941 aatcaaaata gcataaattg tgatctattc gtcggaaata tgtgcaatgt ccacctaagg
     3001 ttatgaacaa attaaaagca gaaatacatt tgttcaaaac tcacctgcaa aactgaacgg
     3061 gggaaatatg caaacgagtg atacccgcgc gttaccgcta ctttgcgccc gctcggttta
     3121 taaacagtat tcaggggtta atgtcctgaa aggcatcgat tttacgttgc atcaggggga
     3181 ggtccacgcc ctgctcggcg gcaatggtgc cggtaaatcg acgttaatga agattattgc
     3241 cggtattacc cctgctgata gcggtacgct tgagattggg ggcaacaact acaccagatt
     3301 aacgccagtt catgctcatc agttgggtat ttatctcgtt ccccaggaac cgctgctttt
     3361 cccaagcctg tcgataaaag aaaacatcct gtttgggctg gcaaaaaaac agctctccat
     3421 gcagaaaatg aagaacttgc tggcggcgct gggctgccag tttgatctgc atagtctggc
     3481 aggatcgctg gatgtcgccg atcgccaaat ggtggaaatc ctccgcgggc tgatgcgcga
     3541 ctcgcggatt ctgatcctcg atgaacctac cgcctcgctt acccctgcgg aaaccgaacg
     3601 cttgtttact cgcttgcaag agctgcttgc tactggcgtg ggtattgttt ttatctcgca
     3661 taagctgccg gaaattcgcc agattgccga tcggattagc gtgatgcgcg acggaactat
     3721 cgccttaagc ggcaaaacca tcgaactgtc taccgacgac attattcagg ccatcacgcc
     3781 agcgctacgg gaaaaatcgc tctctgccag ccaaaaatta tggctggagt tacctggcaa
     3841 cctcgcacaa catgccgccg gaacgtcgat gctgacacta gaaaatctga ccggcgaagg
     3901 tttcaggaat gtcagcctga cgctcaatgc cggagaaatt ctggggctgg ctgggctggt
     3961 gggggccgga cgcacagaac tggccgagac gctctatggt ctgcgtactt tgcgtggcgg
     4021 acgcattatg ctgaatggta aagagatcaa taaattatcc actggagaac gtttactgcg
     4081 cggtctggtt tatctgccgg aagatcgcca gtcatccgga ctgaatctcg atgcttcgct
     4141 ggcctggaac gtctgcgccc ttactcataa ccttcgtgga ttctgggcga aaaccgcgaa
     4201 agataatgcc accctggaac gttatcgtcg ggcgctgaat attaaattta accaaccgga
     4261 acaagctgca cgcacattat ccggtggcaa ccagcaaaaa atcctcattg ccaaatgcct
     4321 ggaagcttcg ccgcaagcat tgattgtcga tgagccgacg cgcggcgtgg atgtctcggc
     4381 ccgtaatgat atctaccagc tgttgcgcag catcgccgca caaaatgtgg ctgtgctgct
     4441 tatctcctca gacctggaag agatcgaact gatggcagat cgtgtgtatg tgatgcatca
     4501 gggcgaaatt acccactctg cactgaccgg gcgcgatatt aatgtcgaga ctattatgcg
     4561 cgttgccttc ggcgatagtc agcgtcagga ggcatcatgc tgaagtttat tcagaacaac
     4621 cgtgaaatca cggcattgct ggcggtggtg ctgctgtttg tattacccgg ttttctcgac
     4681 cgccagtatt taagtgtgca aacgctgacc atggtttata gcagcgcgca aatcctgatc
     4741 ctgctggcaa tgggcgccac gctggtaatg cttacgcgca atattgatgt ttcagtgggt
     4801 tcgattaccg gaatgtgcgc ggtgctgttg gggatgttac tgaacgcggg gtattcacta
     4861 cctgttgctt gtgtcgcgac tttactgctt ggtttgctcg cgggattttt caacggtgtc
     4921 ctggtcgcgt ggctaaagat ccctgccatt gtcgccaccc ttggcacgtt agggttgtac
     4981 agaggcatca tgttgctgtg gactggcggt aaatggattg agggattacc cgccgaactg
     5041 aaacaactct ccgccccgct gctgtttggc gtttccgcga ttggttggtt gacgataatt
     5101 ctgatggcat ttatggcctg gctgctggcg aagacggcgt ttggacgcag tttttatgcc
     5161 acgggcgata atttacaggg cgctcgtcaa ctgggcgttc gtacggaagc cattcgcatt
     5221 gtggcatttt cgttgaacgg ctgcatggcg gcactggcgg gaattgtgtt tgcttcgcag
     5281 attggtttta tccccaacca gaccggtacc gggctggaga tgaaagcaat tgcagcctgc
     5341 gtgctgggcg gcattagttt gctcggcggt tccggcgcga tcatcggtgc ggtactcggc
     5401 gcatggttcc tgacgcagat cgatagtgta ctggtgctgt tgcgcattcc ggcatggtgg
     5461 aatgatttta tcgcgggcat ggttctgctg gcggtgctgg tgtttgatgg acgcctgcgt
     5521 tgtgcgctgg aacgtaatct gcggcggcaa aaatatgccc gctttatgat gccaccgcca
     5581 tccgttaaac ccgcttcgtc aggtaaaaaa cgggaggccg cgtaatgcgt attcgctacg
     5641 gttgggaact ggctcttgcc gcactgctcg ttatggagat tgttgcattt ggtgcaatta
     5701 acccgcggat gttagatctc aatatgttgc tgttcagcac cagtgacttt atttgcattg
     5761 gcattgtcgc cctaccgctg acgatggtga ttgtcagtgg cgggatcgat atttcgtttg
     5821 gttcgactat cggcctctgc gccattgcat tgggcgtact gtttcaaagt ggtgtgccga
     5881 tgccgctggc gattcttctg accttactgc tcggcgcatt gtgcgggttg atcaacgccg
     5941 gattaattat ctataccaaa gttaacccgc tggtgattac gcttggcacg ctgtatctgt
     6001 ttgccggaag cgccctgctg ctttccggga tggccggagc gacggggtac gaaggtattg
     6061 gtggattccc gatggcgttt acagatttcg ctaacctgga tgtgctggga ctccctgttc
     6121 cgctgattat cttcctgata tgtctcctcg ttttctggct ctggctgcat aaaacccatg
     6181 ccggacgtaa cgtgtttttg attggacaaa gcccgcgcgt ggcgctttat agcgcgattc
     6241 cagttaaccg taccttatgt gcgctctatg ccatgacggg gctggcgtct gcggtcgccg
     6301 ctgtgctgct ggtgtcgtat tttggttcag cacgttccga tctcggtgcg tcgtttctga
     6361 tgcccgccat caccgccgtg gtgcttggcg gtgccaatat ttatggtggt tccggttcca
     6421 ttatcggcac cgccattgcg gttttactag tgggatattt gcaacaaggt ttgcaaatgg
     6481 caggagtgcc aaatcaggtg tccagcgccc tttccggtgc gctacttatc gtcgttgtcg
     6541 taggtcgttc cgttagcctg catcgccagc aaattaaaga gtggctggcg cgtcgggcca
     6601 ataacccatt gccataaagg atatcttcat gacacttcat cgctttaaga aaatcgcctt
     6661 acttagcgcg cttggcattg ccgcaatctc tatgaatgtg caggccgcag agcgtattgc
     6721 atttattccc aaactggttg gcgtgggatt ttttaccagc ggtggcaacg gcgcacaaca
     6781 agcgggtaaa gagctgggcg ttgatgtgac ctacgacggg ccgacagaac ccagtgtttc
     6841 tggtcaggta cagttgatta ataactttgt caatcaaggt tataacgcca ttatcgtttc
     6901 tgcggtttcg cctgatggct tgtgtccggc actgaaacgc gccatgcaac gtggtgtgag
     6961 agtgctgacc tgggactctg atactaaacc ggagtgccgc tcttactaca ttaatcaggg
     7021 aacgcccgcc cagttgggag gtatgttggt ggatatggcg gcgcgtcagg tgaataaaga
     7081 caaagccaaa gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa accagtgggt
     7141 gaaagaagcg aaagctaaaa tcgccaaaga gcatcctggc tgggaaattg tcactacgca
     7201 gtttggctat aacgatgcca ctaaatcatt acaaaccgca gaaggaatat taaaagcgta
     7261 tagcgatctc gacgccatta tcgcccccga cgccaacgcc ctgcccgctg ccgcacaagc
     7321 cgcagaaaac ctgaaaaatg acaaagttgc gattgtcgga ttcagtacgc caaacgtgat
     7381 gcgtccgtat gtggaacgcg gcacggtgaa agaatttggc ctgtgggatg tggttcagca
     7441 aggcaaaatt tcagtttatg tcgcggatga attattgaaa aaaggatcaa tgaaaacggg
     7501 cgacaagctg gatattcagg gcgtaggtca ggtggaagtc tcgccaaaca gcgttcaggg
     7561 ctatgactac gaagcggatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa
     7621 caaagagaat atcggcaaat acgatttctg atgtgcatta cttaaccgga gtacgttatg
     7681 gcagatttag acgatattaa agatggtaaa gattttcgta ccgatcaacc gcaacaaaat
     7741 atccctttta ccctgaaagg ttgcggtgca ctggattggg gaatgcagtc acgcttatcg
     7801 cggatattta atccgaaaac gggtaaaacc gtgatgctgg cctttgacca tggttatttt
     7861 caggggccga ctaccggact tgaacgcatt gatataaata tcgccccgct gtttgaacat
     7921 gccgatgtat taatgtgtac gcgcggcatt ttgcgcagcg tagttccccc ggcgaccaat
     7981 aagccggtgg tactgcgggc gtcaggtgca aactctattc tggcggaatt aagtaatgaa
     8041 gccgtggcgt tatcgatgga tgacgccgtg cgcctgaaca gttgcgcggt ggcggcgcag
     8101 gtttatatcg gtagcgaata tgaacaccag tcgatcaaaa atattattca actggtcgat
     8161 gccggaatga aagtgggaat gcccaccatg gccgtgacag gcgtgggcaa agatatggtg
     8221 cgcgatcagc gttatttctc gctggctacc cgaatcgccg ctgaaatggg ggcgcagatt
     8281 atcaaaactt attatgtcga taaaggtttt gaacggattg ttgccggatg tccggtaccc
     8341 attgttattg ctggcggtaa aaagttaccg gagcgcgaag cgcttgaaat gtgctggcag
     8401 gctatcgatc agggcgcttc tggtgtggat atggggcgta atattttcca gtctgaccat
     8461 ccggtggcga tgatgaaagc cgtacaggcg gtggtacacc ataacgaaac ggctgatcgg
     8521 gcatatgaac tctatctgag tgaaaaacag taactgcgga tttaaggaga agaattatgc
     8581 acgtcacact ggttgaaatt aacgttcatg aagacaaggt tgacgagttt atcgaagttt
     8641 ttcgccagaa ccacctgggc tcggtacagg aagaaggcaa tttgcgcttc gatgtcttac
     8701 aggacccgga agtgaattcg cgctttttta tctacgaagc ctataaagat gaagacgcag
     8761 tggcgttcca taaaaccacg ccccactaca aaacctgtgt cgcgaaactg gaatctttaa
     8821 tgaccgggcc gcgtaaaaaa cgtctgttca atgggttgat gccgtgaggc gaatttatca
     8881 attttatcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg actggaaccc
     8941 ctctttatat ctgcactttg ccgctgaacg atcgcgtccg gcggtggagc tgcttgccag
     9001 agtgccgctg gagaatatca aatacgttgc cgatcttggc tgtggcccag gtaacagcac
     9061 cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcgtcgact catctccggc
     9121 gatgattgct gaagcgcgca gtgctttgcc agactgcctg tttgtggaag cagatatccg
     9181 caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac tgcaatggct
     9241 gcccgaccac tacgaattgt ttcctcattt ggtttcgtta ctcagtccgc ttggcgtgct
     9301 ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc gcgaagttgc
     9361 ctgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc atgcttacta
     9421 cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccacct actatcacca
     9481 gatgccgtca caccaggcga ttatcgattg ggtgaccgcc actggattac gtccgtggtt
     9541 acaggatctg accgagagcg aacagcagct ttttcttaag cgctaccatc agatgctgga
     9601 agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc gtctttttat
     9661 tgttgcccgc cgtacggagt aaatatcacg tcagctggta atgacgatcg ggaagaattt
     9721 ttgctggaat ttcggcttca tcgttcatct gtaacaggtc aatttcaata gcgttgcaga
     9781 tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt tcttccgcca
     9841 gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc gtcatgtaat
     9901 gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg gtacgatgca
     9961 aaatcagggt gtaggcaaag ggaattggcg tataggcaat gcgctcgcat cctgccagga
    10021 ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct gaaagctgcc
    10081 cattgcggcg ctgaaccgcc aaccactccc ccattattaa caagatacgg ttagctggag
    10141 agttcgaagc cagtacaggc tgaagatctt cagtcttgag ataatgagcc agcacttccg
    10201 cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt tgcaaccggg
    10261 caaactccct tacacttgcc gaatccggca atgtcgtttt tacctcgcgc agtaacgacc
    10321 gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg gcgtacccgg
    10381 cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg aacggtgcga
    10441 gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaaaataaca gcgatagaaa
    10501 agagaaaatt gagtagtaag cgcgatgata tcttggataa tactgagccg tgccagacaa
    10561 aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc aggcgtgaaa
    10621 aagtcgccct attaaacgtg attacgatca cattctcaag acgctattta caaattacct
    10681 acaagcttac aacattaaca ggcgctgcat gtggcatctg ttaatgataa aggcacatag
    10741 gatgttgtaa ctaatatagt gaaataagaa acccggtcga aaccgggttc agaagtaacg
    10801 gtgttattag cacaacggac gtacagcttc gcgcatccct ttttcaagaa tcgcatccag
    10861 gtcattagca acctgctcga ccagaccagg cacttgcgtc aggtcctgct cccagtggtc
    10921 tttttctgcc aacacaatcg ctaccagttc ctgggtaccg ataacacggt cacgatgctg
    10981 gctccagagt tgctgataac gttccagcca gtgtgcatcg tcctgtaccg gatatgtttc
    11041 tccgttacgc tcaccacgat agaacgcaat caatgccgct aatgcgaaag taaggcgcgc
    11101 cggaagtgtg ccgtttgcct tctgccctgc cagcagctgc ggcaggatgc gggtgcggaa
    11161 cttggtcata ccgttgagcg cgatagacag cagctgatgc ttaatgtacg ggttacggaa
    11221 acgcccggtg actgcactgg cgaaagattc cagttcatca cgaggcaaat ccagtaccgg
    11281 aataatttct tcgtagatgg ctttttcaac gaatgcgcaa atttcagcat cgttcatcgc
    11341 ttcacctacg gtatccagcc ctgcctggaa cgccaccggc accagcgcgg tgtgcgcacc
    11401 gttgaggatt gccactttgc gttctttata cggtttaata tcgtcaacaa tcagcacgtt
    11461 gagcggatat ttgtccaggc gcagctcagt cgctaaggat ttcggcccct gaatcacaaa
    11521 caggtaaaag tgttcagcgg tgtcgaggaa accatcgtga taacccagtt cttcttccag
    11581 tttagccact tcatcgcgcg gataaccggt aacgatacgg tccaccagcg tagaacagaa
    11641 gctgttagcc tgatccagcc attgaataaa tgcttctggc agtgcccact cttgcgcata
    11701 gcgcagcacc agttcacgca acgcgtcgcc attgtagtca atcaactcac acggaatgat
    11761 gatccaacct ttatccagcg caccgttgaa atggctaaag cgttcgaaca gcagacgggt
    11821 cagttttgcc ggatagctta ccgctggtgc gtcatcgaat ttatcgcccg catggtagct
    11881 gatccccgct tcggtggtgt tagagaaaac aaagcgcatt tccgggttgt gcgccagttt
    11941 caggaattca tcgtattcac tgtagacgct gatttcacga ttaaccgagc gaatcagacg
    12001 cgcgtcgctg accgcttccc ccttctcatt caggccacgg ataatggtgg tgtacagacc
    12061 atcctgcgtg ctcagtgacg gcgggaatga agtttcaatc ggacgaacaa cgaccacgcc
    12121 agaattcaga tcggtgtgct cattcaggag atcgatttgc cagtcaacaa aggcgcgcag
    12181 gaagttacct tcaccaaact gaatgatacg ttctggatac tgtgcaccgg gaaaatcgcg
    12241 acgatttagt gttttcacaa tgggttccct tctgattagt catacaacct gtttgaattg
    12301 gtacgacagg ttagcaaact ttaatacgcc gaacccctgt tttgatcaac tcctgatgat
    12361 taatgagcag ttttatgaga aaagtgtggc gcggatcatg gtttaatcga ggaaatacgc
    12421 cttttcctgg atcataaagt ggtagaacac attgcattca aatcgcgcgg aatgaataaa
    12481 gatgtcagac aacttcctca ccgtaacgca tagtgctggt acggttgcgc ccatctttct
    12541 tcgagcgata cagacacgta tcggcttcga ccatcaattt attaaaatca tcggtcagcg
    12601 ttcggtacga tgcgcgacca ctaccgacgc caatacttac cgtgagataa agcgtttttt
    12661 gttgccaggt gaatggttgc agttcaacgc ctttacggat tttttccgcc attagcagac
    12721 catcgacagg attcaccgac ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca
    12781 ccagcccctt atcaccgaca atcttctgaa tatgccgggc aaacacgctt aacactttat
    12841 cgccacattt atgtccatag ttatcgttaa tacttttgaa atagtcgata tcaagcagca
    12901 tgacagtcag atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg
    12961 accgtgagta aacctgagtc agaaaatcaa agtcggctcg cagcgcaact tgcttcatta
    13021 gcgaattgat cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa
    13081 taccgagacg tgcggagaac atttccggaa tggagaacgg cgaaccgacc gagatatcaa
    13141 tcaccgaatt agccaccagt acgatttcca ctgcaccggt aacaaaggtc aacagacatg
    13201 ttacctgcgg cgtatagcgc actgcacacc agattaatgc aggcagcggg aacgccagac
    13261 tccccgctcc gccaatgact accgaggcaa taaccgacac aataagcgcg atggcgggca
    13321 tcatctgttc tgctttaaag cgcggcagca ctccaggaat tgccaacgtc agcatacaag
    13381 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
    13441 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
    13501 caatcagaca gtaattaaat agccgtaagg cacttactgg ctcatactta tttttcccca
    13561 gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
    13621 tgatagccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
    13681 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg
    13741 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
    13801 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
    13861 cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
    13921 aattgcgggt ggcaattatc tcaacgtaat aagtaaattc tcataaagga tagcatgttc
    13981 gctgacagcg gcggcaaata aaggtaccct aggaatgcta ttttatcgtt ttccgcaatt
    14041 atcatggctg attcatctgt ataaccagcc agtgatagat taccgccacg atataacgtt
    14101 gttgttgctc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaacagc
    14161 gacaacaggt tgcagtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
    14221 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
    14281 gttgttcgat gactggcgca cctttatcca gacaatactg acgctctttc atgcccaaac
    14341 gaaagcgaga tcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agagactgca
    14401 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcaa tgcccgcaag
    14461 cgagttacca gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
    14521 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
    14581 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgtgcctg
    14641 cattggcgtc accacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
    14701 aaacgtccgg gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg
    14761 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
    14821 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt aggaaatatc
    14881 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttaatcg
    14941 cccttgcaac atatcgcaga ccaccagcgc accggcatta ataaacggat tgcgcggaat
    15001 accctgctcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
    15061 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
    15121 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
    15181 gtcaacggta cagatagcaa tgcccaatcg ggaaccgtct accgtagcca gcgccggaat
    15241 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
    15301 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
    15361 tgcgagtata tcagagctaa atatgtcgcg tcagatccgg tctttccaca ccgtctggat
    15421 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
    15481 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
    15541 ttccagacgt gccgccatct gtctggcccg tgtttcgtcg gtggtaaaaa tggtcgctga
    15601 aaggccgaac tcactatcat tagccagttc cagtgcatgt tctgcatctt tcgcaatggt
    15661 gattgccgca acggggccaa acatttcttc ccgaaatgcg gtcatttctg gggtaacatt
    15721 cgccagaacc gttggcggat agtagttacc tgccccagcc atcttttccc cgcccagtaa
    15781 caaatgcgca ccctgcgcca gggttttctc cacctgatga tgcagctcat cacgtaaatc
    15841 aaaacgagcc attggtccga gagtgttctc ttcgtcacgg ggatcgccca ttttcaaggc
    15901 tgccgcagct gccacaaaac gttcggtaaa tgccgaagca attccctctt cgatgataaa
    15961 acgttttgcc gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt
    16021 caccgccagt tccagatcgg catcgttaag cacaataaac ggatccgaac cgcccagttc
    16081 cagtacgcat tttttcagtg ccgctccagc ctgtgcgcca atagccgctc cagcacgaac
    16141 acttccggtc accgtgatag cagcaatgcg cgagtcttta atcatctgac tgacaccgtc
    16201 gttgtcggca ttcagccagc catatacgcc ttgtgggata cccgcatctt taaacacctg
    16261 ggcaatgagc tgtgcacagc ccatcacatt cggcgcatgt ttaagtaagt agccgttacc
    16321 tgcaagaatg atgggaacag cgccacgcat cacctgccat aacggaaaat tccacggcat
    16381 aatcgccaga atcgtcccca acggtcgata ctcaataacc gcctgctgat tttccaccag
    16441 cgtaggttcc gccttcagca ttgccggacc atgttctgca taccagtcac acaaattcgc
    16501 cgatttcgcc acttcagcgc gcgcctggtt gattggtttg cccatttcgc tggtgatcat
    16561 ttgcgccatt tcttcgctac gagcgcgcag agccttaccg atatcacgca gtttttcagc
    16621 acgataatct atatttgtct cgcgccagtc acgaaagcct gctgccgcca gctgaagtgc
    16681 gttttcgata tcatcagcgc cagcccacgg caacacagaa agttgttcac ccgtggcagg
    16741 atttatcgaa attgcatgag ttgccggagt aatggtcatc ggggtatctc ctttatgagt
    16801 catggtatga agatacgcag atttactctt gctttaaaat gaataatatt aagccactta
    16861 ttcacgaatc gagaatgtta tggatctgac ccaactggag atgttcaacg cggttgccga
    16921 agctggcagc attacgcagg ctgcagcaat agtgcatcgc gtgccgtcga atctcactac
    16981 ccgtctacgc cagctggaaa cagaactggg ggtagatctg tttattcgcg agaatcagcg
    17041 tttacgtctc tctcctgccg ggcataactt tttacgctat agccaacaaa ttctcgcgtt
    17101 agtggatgaa gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat tttctctggg
    17161 ttcgctggaa agcaccgcag cagttcgcat tccggccacg ctggcggaat tcaaccgtcg
    17221 ttatcccaaa attcagtttt cactttccac cggcccttcc ggcactatgc tggaaggagt
    17281 gctggaggga aaactgaatg cggcatttat tgatgggccc attaaccata ctgccattga
    17341 cgggataccg gtataccgcg aggagctgat gatcgtcacg ccacaaggtc atgcgccagt
    17401 aatccgcgcc agtcaggtta acggcagtaa catttatgcc ttccgcgcca attgttcgta
    17461 tcgtcgccac ttcgagagct ggtttcatgc tgacggtgcc gctccgggaa ctattcacga
    17521 gatggagtcg tatcacggga tgttggcctg tgtggtcgca ggagcaggca ttgcgcttat
    17581 tccacgctct atgctggaaa gtatgccggg gcatcaccag gttgaagcgt ggccgttagc
    17641 tgagcaatgg cgttggttaa caacctggct ggtctggcgt cgtggtgcga aaacgcttcc
    17701 gcttgaggca tttattcaac tactggatgt tcctgactcg gcaaaacagg gttaccaatg
    17761 agctattttt gatagttctt gcagctcaaa tgccctatat agtgatgttc cgatgactta
    17821 tgactatatg gggcaaatat ggttacgcca gtaagcatca gcaattacat atctcttcct
    17881 gataattttc ccgctcgtaa tattgcacct caggtaaaag aagttttaaa ggattttatt
    17941 gatgcactca gtaccataat atgtgatgaa gagtggcgca cgtttttaaa catcaactca
    18001 gccacaaaaa agatatttaa caaccttgat aacttatcat atattcagag gacttctttt
    18061 cggggtaacg acacactata caatgaaaag gttcagttta aactcactta tcccgtcaag
    18121 aatggaagac acaaagaaaa tattgaattt caggtagtaa taaatttaag ccctatttat
    18181 ttagataatt ttcgccatga tggagaaatt aatatttttt gcgctcccaa cccaaagcct
    18241 gtcactatgg ggcgcgtatt tcagaccggc gtcgagcgtg tgctctttct gtttctgaat
    18301 gattttattg aacaatttcc aatgatcaac cctggtgttc ctatcaaaag agcgcatacg
    18361 ccacatattg aacccctgca tccggatcac cataccgctg cagattactt acgccagttt
    18421 gatttgcttg tcctgaattt tatctctcgc ggtaattttg tcatactccc ccgattatgg
    18481 aataactctg aggttcacag atggttcggg aataaagaac ctaacttgct caccgctatt
    18541 ctcgacataa cggacagcga attaaaagag gatttattgc aaagtctgat ggattcacta
    18601 ggttctaaca aacatgtact acccgaagtc tgcatctgct ttttatccct tttagcagaa
    18661 caagattttc cacattttca ggacttgttt ttatttttcg ccaatatgtt attgcactat
    18721 caccaattta tgaatcccaa tgaaagtgat ttgattgacg tgttgatgcc agcatcatta
    18781 agtgatgata aaattatcaa acatatggcg cgcaagaccc tcaaactttt tgtaaaaaat
    18841 gaaacgccgc caaaagttac tcatgaagac ctggtgaaaa acaggcctcg ctcccctgtc
    18901 agaccaccta tacccgcaac ctccaaaacg ccagacctcc ctgaacgtca ttaaaccgtg
    18961 atgtgaccga ctctctgacg cgtgaacgaa tcagcgtcag agaaacggaa aacgcgatcc
    19021 agatcacaaa tgcattgtat tcacatcatt aaccgtttta agatcatttc atcacttttt
    19081 cgcaactcac ccgataatct gttatgacaa caaacactgt ttcccgcaaa gtggcgtggc
    19141 tacgggtcgt tacgctggca gtcgccgcct tcatcttcaa caccaccgaa tttgtcccag
    19201 ttggcctgct ctctgacatt gcgcaaagtt ttcacatgca aaccgctcag gtcggcatta
    19261 tgttgaccat ttacgcatgg gtagtagcgc taatgtcatt gccttttatg ttaatgacca
    19321 gccaggttga acggcgcaaa ttactgatct gcctgtttgt ggtgtttatt gccagccacg
    19381 tactgtcgtt tttatcgtgg agttttaccg ttctggtgat cagtcgcatt ggtgtggctt
    19441 ttgcacatgc gattttctgg tcgattacgg cgtctctggc gatccgtatg gctccggccg
    19501 ggaagcgagc acaggcattg agtttaattg ccaccggtac tgcactggcg atggtattag
    19561 gtttacctct cgggcgcatt gtggggcagt attttggctg gcgaatgacc ttcttcgcga
    19621 ttggtatcgg ggcgtttatt acccttttgt gcctgattaa gttacttccc ttgctgccca
    19681 gtgagcattc cggttcattg aaaagcctcc cgctattgtt ccgccgcccg gcattaatga
    19741 gcatttattt gttaactgtg gtggttgtca ccgcccatta cacggcatac agctatatcg
    19801 agccttttgt gcaaaacatt gcgggattca gcgccaactt tgccacggca ttactgttat
    19861 tactcggtgg tgcgggcatt attggcagcg tgattttcgg taaactgggt aatcagtatg
    19921 cgtctgcgtt ggtgagtacg gcgattgcgt tgttgctggt gtgcctcgca ctgctgctac
    19981 ctgcggcgaa cagtgaaata cacctcgggg tgctaagtat tttctggggg atcgcgatga
    20041 tgatcatcgg gcttggtatg caggttaaag tgctggcgct ggcaccagat gccaccgacg
    20101 tcgcgatggc gctattctcc gggatattta atattggaat cggcgcgggt gcgttggtag
    20161 gtaatcaggt cagtctgcac tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg
    20221 cttttgccgc gttaatatgg tcaatcatca ttttccgccg ctggccagtg acactcgaag
    20281 aacagacgca atagttgaaa ggcccattcg ggcctttttt agtggtacgt tttaatgatt
    20341 tccaggatgc cgttaataat aaactgcacc cccatacata ccagcaggaa tcccatcaga
    20401 cgggagatcg cttcaatgcc ccccttaccc accagccgca taattgcgcc agagctgcgt
    20461 agacttcccc acaaaataac cgccaccagg aaaaagatca gcggcggcgc aaccatcagt
    20521 acccaatcag caaaggttga actctgacgc actgtggacg ccgagctaat aatcatcgct
    20581 atggttcccg gaccggcggt acttggcatt gccagtggta caaaagcaat attggcgctg
    20641 ggttcatctt ccagctcttc cgacttgctt ttcgcttccg gtgaatcaat tgctttctgt
    20701 tgcggaaaga gcatccgaaa accgataaac gcgacgatta agccgcctgc aattcgcaga
    20761 ccgggaatcg aaatgccaaa tgtatccatc accagttgcc cggcgtaata cgccaccatc
    20821 atgatggcaa agacgtacac cgaggccatc aacgactgac gattacgttc ggcactgttc
    20881 atgttgcctg ccaggccaag aaataacgcg acagttgtta atgggttagc taacggtagt
    20941 aacaccacca gccccaggcc aattgcttta aataaatcta acattggtgg ttgttatcct
    21001 gtgtatctgg gttatcagcg aaaagtataa ggggtaaaca acgataaagt gtcactcttt
    21061 agctagcctt gcatcgcact gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt
    21121 caattcattc atttgactta tacttgcctg ggcaatatta tcccctgcaa ctaattactt
    21181 gccagggcaa ctaatgtgaa aagtaccagc gatctgttca atgaaattat tccattgggt
    21241 cgcttaatcc atatggttaa tcagaagaaa gatcgcctgc ttaacgagta tctgtctccg
    21301 ctggatatta ccgcggcaca gtttaaggtg ctctgctcta tccgctgcgc ggcgtgtatt
    21361 actccggttg aactgaaaag agtgttatcg gtcgacctgg gggcgctgac ccgtatgctg
    21421 gatcgcctgg tctgtaaagg ctgggtggaa aggttgccga acccgaatga caagcgcggc
    21481 gtactggtaa aacttaccac cagcggcgcg gcaatatgtg aacaatgcca tcaattagtt
    21541 ggccaggacc tgcatcaaga attaacaaaa aacctgacgg cggacgaagt ggcaacactt
    21601 gagcatttgc ttaagaaagt cctgccgtaa acaaaaaaag aggtatgacg atgtccagac
    21661 gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac aacctggaat
    21721 cgccactgtc actggagaaa gtgtcagagc gttcgggtta ctctaaatgg cacctgcaac
    21781 ggatgtttaa aaaagaaacc ggtcattcat taggccaata catccgcagc cgtaagatga
    21841 cggaaatcgc gcaaaagctg aaggaaagta acgagccgat actctatctg gcagaacgat
    21901 atggcttcga gtcgcaacaa actctgaccc gaaccttcaa aaattacttt gatgttccgc
    21961 cacataaata ccggatgacc aatatgcagg gcgaatcgcg ctttttacat ccattaaatc
    22021 attacaacag ctagttgata acgtgacaac gtcactgagg caatcatgaa accactttta
    22081 tccgcaatag cagctgcgtt tattctcttt tccacgcagg gcattgcgga acaaaccacg
    22141 cagccggttg ttacttcctg tgccaatgtc gtggttgttc ccccatcgca ggaacaacca
    22201 ccgtttgatt taaatcacat gggtactggc agtgataagt cggatgcgct cggcgtgccc
    22261 tattataatc aacacgctat gtagttattg tcaggctctg atcacagagc ctgatatctc
    22321 cgtaatatct tcctgaagtc tcaaatattt tcgccattgt tggcgatgag atccctatac
    22381 cagtaaaacg acgctttacg tatccgttcc agggttccct tcccttcgtt gtctttatcg
    22441 acgtaaatca ttccgtagcg ctttttcatt tcccctgttc cggcagaaac caggtcaata
    22501 cagccccatg gggtgtagcc aattaagtca acaccatctt cgactacggc ttttttcatt
    22561 tcccgaatat gggaagcaaa gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct
    22621 tgtctctggt caaccgcacc aaatccattt tcgacaataa acagcggcaa ctggaaatga
    22681 tcccagaacc agttgagtga ataacgtagc cctgccggat caatttgcca tccccattcg
    22741 gatgtatcga tataagggtt gctgaccaaa tctcgtggtt caacataatt cagttgcggg
    22801 ttatctggcg aaaattgcgt agtaaaagac atgtagtagc taaagccgat aaagtcgaca
    22861 caacctctgg caagaatcgc attatcttct ggtgtgatat cgaggttgaa tcctttcctg
    22921 gcaaagtaat tcagcatatg ttgcggataa tatccacgcg catgaacatc agtaaaccag
    22981 taacgacgat gcatcgcttt cgtggccatc atcatatcgt tgggtgcaca agtcagagga
    23041 tagatggggc acatggcgat catacagccg atattaaatt caggattgat cgatttacca
    23101 gtctgtactg ccagggcact ggcaactaac tcgtaatgta ccgcctggta cattatttgt
    23161 tcgcgctcat tgatatcttc ctctggcgaa tacaagatac cggaattagt aaatggacac
    23221 aggctttcgc tgaaattcac ctgattattg atttcgttaa acgtcatcca gtactttact
    23281 ttttctttat agcgcgtgaa gaccgttgat gcgaagcgga tgaaaaagtc gatcagttta
    23341 cggtttcgcc agccaccata ttttgtcacc agatgataag gcatctcaaa atgcgaaagc
    23401 gtcaccacag gttccattcc ctgcttcagg cattcatcga acagatcatc ataaaatttt
    23461 aaaccctctt cattcggctc ctgttcgtca ccttgcggaa agattcgtgt ccaggcaatg
    23521 gaagttcgaa agcatttgaa tcccatctcg gcaaataact gaatatctgt tttatagcga
    23581 tgataaaaat caattgcttc atgattggga taattaagtc cgtcgataac gccttctgtc
    23641 acttcacgcg gcacaccgtg agcgccagca gtcatcacat cagcgatact gatgcctttt
    23701 cctccttcat tccagccacc ttccaactga tgcgcggcta ccgcgccgcc ccataaaaaa
    23761 ccttttttaa atcctgacat acacaactcc ttaactaaaa tagattacca ccagacttcc
    23821 gcctggatgc cggccataaa ctcgtctttg ctgttctcat taaacttaaa attcgataat
    23881 tcgttatcca gaatattgat atacgttcca tagaagcgaa tttctggtcg tgaacctaaa
    23941 atgctttcac ccactttcaa tgcatgccag agtgtcgttt tataagccga ttcatttagt
    24001 gttacaccct gctgtgtttt gttctgttgc ttaaaccagc ctaattcaag ccccgtctga
    24061 ttccatgtat tccagatcca ggccggtctt actacggtgc gaatactgtc aaaatcacta
    24121 tgagcgccac tttcataact ataaacatct tcgccatgag aatagacaag cgcgttagcc
    24181 ataataatat tgtcagtaag atacatctcg ccctgagaga ttaaacgcca ggcgatccca
    24241 ttggtatggt caccatagta atagcgacca tgcgccatcg tgttactggc atctgagaaa
    24301 ctggcaaaac tgctggcata ggaattattg gcaacctgta acgtaaattc attaaacgta
    24361 tcgcgttgca ggttttgccg taaaacagaa gtcagcatcc agctatcttt catttcaaaa
    24421 taactgtcgt cattttcatt atcttgttgt tgatccgttt tatttggtgc ggaatattta
    24481 gccattaatg ataatgttgc atcatcccat aacgggatat tgcgataacg aacatccaca
    24541 gaattggtat tcatctgact ggtacgcgta aaatcacgag agtaaacatc gacatcatca
    24601 cggcttaagg acatatcaaa cagccctaca ccaagtgccc agttttcaat ccccacaccc
    24661 gcagcgacat ccgtggttaa ggttttccag tccagcattt ggatctcata ttgcgggagt
    24721 ttatgtttgc ctacccagaa gtctgcctct ggcgcgaagg gtaaaaaacc tcgcgttgtc
    24781 agataaatat cactgaactg catgatattt tcattggcac tgtcaccaaa ccaggcatcg
    24841 ttatactgct cacctacgtt gccatcatag gtaacgaccg catttgccgt tttaccgtcc
    24901 tgattataaa cacgctgatt taaggttaag tcaaaccaac cgctcatctc gttaccaaaa
    24961 cgtcccagag aacccgctgc ataagtttgt ggtgagccat gatttccggt accccagcct
    25021 gagcggaagt acccctgata gctgaaccca atatcatcct ttatatattt actgatatct
    25081 ttcagggtca cgctttcgat agtggttttg tcgcctttac tttcaataac ggcaacttgc
    25141 tgcgatccat ttatagtatg cgctgcggca gacgagttct gttcaccgtt ttcatttttg
    25201 atgttatcag cgacgggaga tgtcgctgat atttcagcaa gtgaggctga tttgtattta
    25261 ttttccgtaa tacttttttg taaggtcgaa agtcgggatt tatatacccc cagttcattc
    25321 tgtgttgctc tcagctcttg tttattttgc gacaattcat tttcaagcag ttcaaggcgc
    25381 tgctcaatgg ttaattttgt tgccaacgcg ttcaacggaa acaatgctgt aataatagaa
    25441 aaagacaaaa ggctgacgtt aatcgtcttt atattcatga cttatcctta agagtgtaat
    25501 aatttcctcc agctatcgca ctggaggaaa aaatcaggca tggagctctt ttcttaatga
    25561 aataatttcc ctggctaaat cctggcataa tattgcattc atcaaatgat cctgtgcatg
    25621 aaccagaatt aaattaactt ctattttgcc acaaccttca tcctgactaa ttaacgcggt
    25681 ctgaatctta tgtgcctgaa gacaggctaa ttccgaactg gccattagct gagttgcgcc
    25741 ctgccagtct ttttgcccgg cacaacgtat ggcttccatc gcatcagaac gtgcctgtcc
    25801 cgcgttgatc agtaactcca taacaagttc ttcatctgca aacatcaaca tctccttcct
    25861 gtgtaaatgg gattatccgt tagttgtcat gttattgacc ttgttggcgg caataacaaa
    25921 aggtaaatag atcattgtcg ccacacctaa acataaaaaa cccaccagta acgccaacca
    25981 gtttcccccg gttccgagga aagcaattaa aggtccgggc gtagtccagg gcacggcata
    26041 agcaaccggt ggaataattt ccatcgaaac aaagaagtaa ccaatggcac agttaacaat
    26101 gggaaccaga ataaacggca ccatcatgat ggggttaagt acaataggaa ggccgaacat
    26161 tatcggttca ttgatattga aaataccggg gataaacgac atttttgcca gatcacggta
    26221 ttccgcacgc ttagacgcga taaaaatagc caacaataac cccaacgtca tacctgaacc
    26281 gccgcagttg gcgaatgcat cattaataga agtccaggta atcggatatg gcgcgcccca
    26341 ggtagtgccg tgttgagcgg cccaggagag attctccaga ttagcctcag aaaaaacagt
    26401 ttctcgaatt gccgaggtgg tattaggtcc gtggatcccc agcacccaca ggaaattacc
    26461 cacagccccc aggataatta ccgcaaagat attcgttccc atatgtttga gcggcgtctg
    26521 gattaatgtg taaatgagat cgtttaatcc tgccggggat atcagtgtca ggcaataatt
    26581 aagtgcggaa aagaacacca tgacaaaaaa tattggcaat aaaactttaa atgaacgcga
    26641 taccgcagga ggtacagctg ccggcatcgt tatggtgatt tttgggtttc gagctaagcg
    26701 acaaaatatt tcactggtga ttaatgcaac gataacagca acaaacaccc cctgtgcgcc
    26761 gagatatttg gtcgtcagga atgcttgccc atctatcatc tgatatggcg tataaacaat
    26821 aaaaaatgct cctattgccg ttaatccgca taaaagatca tcttgctcat agctaatcgc
    26881 catatttctg gcgaccagga aagcaatcat aattgacatg atatttacag agccattcat
    26941 taccggagta aaaatagctt gtgctttttc aaggttgggg aaaacgcggt tcagatgcag
    27001 cagcccggca ataaagccgt cgggcgataa tatggcaaag ttaattaata taattaatga
    27061 gcttgccatg ataattggaa acgtaaaaac gaatccatct ctcaccgctg caacatgttt
    27121 ttgtgagttt aacttgatag caactggaac aagaaaacgt tccattccac gttcgaatga
    27181 ggtcattaat cccatttttt atacctgttg ttctggatat ttatacatcc caataaagca
    27241 tgtatgccat atccatttta tgatgtgtga aaagttagtt caggtaatag tttaataata
    27301 ttagattctt actcttgcag tagtttcatc gcaaattcaa gaactgcctg tccgttcatg
    27361 gtgccatagt ctttcatctc gataacagct acaggcattt tcccctgtgc aacagcttca
    27421 atctctggtt tctgaaagcg aacttgcggg ccaagtaaaa caacgtcaac ttcgttattt
    27481 ttaattttcc ctttagcctc tgcaatcgcc aatgcggaaa tattaacttc aagtgaaata
    27541 gcgttagcat gatcaatcat acgtttaacc agcatactgg ttgacatgcc cgcagcgcaa
    27601 actaacaata tctttttcat attttccctt actggtatat aacagattac attgtgtaaa
    27661 tatcatatcc cgcagtagct atacgtcctg gtacattgat cacagtaata cagcactaaa
    27721 gttgttcaac atcagggtga taattaaacg actcgaccat acgataaacc aatcaggact
    27781 caactccttg ttttcaatgg ctcttaaaag gtgcaaaagt gatttttcaa aagattgccc
    27841 gcttacttaa atccgagatc aatggctatt catggcatgt tggtgatttg ttgccgtcag
    27901 aagctgaact cgctgttcgt tataatgttt cgcgtaatac cctgcgtaag gcattatcct
    27961 tgctggaagg cgaaggaatt attcatagaa agcatggttc aggaacatat attcaaaaaa
    28021 agaattttgt tgcacacatt gatcacatga acagcttcag tgaaattgcg cataaaagcg
    28081 gcaaggaggc aggaagtcag attatgaaat ttgaagtgca ggatgcttct ccccctattg
    28141 cgactgagct gaatttagtg actggtgagc aggtttatta cataaagaga ctgcgattca
    28201 ttgaggataa tgcagcacaa ttagaagaaa cgtggatgtc agtggcacgt tttcctgatt
    28261 taaccgtagc acatatgcaa aaatccaaat tttcgtatat tgagaacgaa tgcgggatca
    28321 aaatcattgg cacctttgaa actttctccc ctacttttcc taccccagaa atcgccagta
    28381 ttttacggat cagcccacgg gatcccatac ttaaaattca gacccaggct gtggacagta
    28441 actctattcc gctggattat tcgttacttt acagcaatat tttcgagttc caggtaaagt
    28501 actttttccc gcgataaaag cagacgcaca gagtgctgac atcacattct gtgcgtatgg
    28561 tttagcctct aacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    28621 cggtcataat gagcaccgcc ccgaaaaatt gtagacccgt taagcgttca tccaacaata
    28681 gtgccgcact tgccagtcct actacgggca ccagtaacga taacggtgca acccgccagg
    28741 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    28801 ccagatacat cagagacaag atggtggtca tatcgatagt aaccagactg tgaatcatgg
    28861 ttgcggaacc atcgagaatc agcgaggcaa caaagaaggg aatgattggg attaaagcgc
    28921 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    28981 agatgttgcc acacgcccaa ctaaatgctg ccgccagggt caacataaag ccgagcatcg
    29041 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    29101 cgatccccgc caattgtttg ccatgcagtc gctcaccgaa agtaaacgcg ccaagcacga
    29161 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccgaagt
    29221 taatggcaca aaaaagaaaa gcaaactgcg caaaactgat ggttaatcca taccccagca
    29281 gcaaattcag cggtactttc ggtcgtgcga caaaaaagat agccggaaaa gcaaccagca
    29341 taaagcgcaa accggccaac atcagcggtg gcatgttatg aagccccact ttgatgacca
    29401 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    29461 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    29521 ttaacagttg attcgttagt caccgtttac gacggcatta atgcgcaaat aagtcgctat
    29581 acttcggatt tttgccatgc tatttcttta catctctaaa acaaaacata acgaaacgca
    29641 ctgccggaca gacaaatgaa cttatcccta cgacgctcta ccagcgccct tcttgcctcg
    29701 tcgttgttat taaccatcgg acgcggcgct acgctgccgt ttatgaccat ttacttgagt
    29761 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    29821 ggcgtcgttt ttagtctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    29881 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    29941 gtgacgctgg tggtgctctt ttttgccctc attaactgcg cctattccgt ttttgctacc
    30001 gtgttgaaag cctggtttgc cgacaatctt tcctccacca gcaaaacgaa aatcttctca
    30061 atcaactaca ccatgctcaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    30121 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    30181 cttttcattc aaatttgggt aaagcgcagc gagaaaatca tcgccacgga aacagtcagt
    30241 gtctggacgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    30301 tttctggctt cctttgttag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    30361 attgctgatg gtgattttgc cgaaaaggtg gttgcggttg ttcttccggt taatgctgcc
    30421 atggtagtta cgttgcaata ttccgtggga cgccgactta acccggctaa catccgcgcg
    30481 ctgatgacag caggcaccct ctgtttcgtc atcggtctgg ttggttttat tttttccggc
    30541 aacagcctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    30601 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    30661 ttttccgctc agtctttagg ctggcttggt gcggcgatta acccattagt gagtggcata
    30721 gtgcttacca gtctgccgcc tttctcgctg tttatcatct tagcgttggt gatcattgct
    30781 gcgtgggtgc tgatgttaaa aggaattcgt gcaagaccgt gggggcagcc cgcgctttgt
    30841 tgatttaagt cgaacacaat aaagatttaa ttcatccttc gtttaggtta cctctgctaa
    30901 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    30961 agggaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    31021 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    31081 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    31141 aaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg gatagatttg tgttttgctt
    31201 ttacgctaac aggcattttc ctgcactgat aacggatcgt tgacacagta gcatcagttt
    31261 tctcaatgaa tgttaaacgg agctgaaact cggttaatca cattttgttc gtcaataaac
    31321 atgcagcgat ttcttccggt ttgcttaccc tcatacattg cccggtccgc tcttccaatg
    31381 accacatcca gaggctcttc aggaaatgcg cgactcacac ctgcagtcac ggtaatgttg
    31441 atatgccctt cagaatgtgt gatggcatgg ttatcgacta actggcaaat tctgatacct
    31501 gcacgacatg cttcttcatc attagccgct ttgacaataa tgataaattc ttcgccaccg
    31561 tagcgataaa ccgtttcgta atcgcgcgtc caactggcta agtaagttgc cagggtgcgt
    31621 aatactacat cgccgattaa atgcccgtag gtatcattaa ccaatttaaa tcggtcaata
    31681 tccaacaaca ttaaataaag attcagaggc tcagcgttgc gtaactgatg atcaaaggat
    31741 tcatcaagaa cccgacgacc cggcaatccc gtcaaaacat ccatattgct acggatcgtc
    31801 agcaaataaa ttttgtaatc ggttaatgcc gcagtaaaag aaagcaaccc ttcctgaaag
    31861 gcgtcgaaat gcgcgtcctg ccagtgattt tcaacaatag ccaacattaa ttcccgacca
    31921 cagttatgca tatgttgatg ggcagaatcc attagccgaa cgtaaggtaa ttcatcgtta
    31981 tcgagtggtc ccagatgatc aatccaccga ccaaactggc acagtccata agaatggtta
    32041 tccgttattt ctggcttact ggcatctctc gcgaccacgc tgtgaaacat actcaccagc
    32101 cactggtagt gggcatcgat agccttattg agatttaaca agatggcatc aatttccgtt
    32161 gtcttcttga tcattgccac tcctttttca cagttccttg tgcgcgctat tctaacgaga
    32221 gaaaagcaaa attacgtcaa tattttcata gaaatccgaa gttatgagtc atctctgaga
    32281 taacattgtg atttaaaaca aaatcagcgg ataaaaaagt gtttaattct gtaaatcacc
    32341 tctgcattat cgtaaataaa aggatgacaa atagcataac ccaataccct aatggcccag
    32401 tagttcaggc catcaggcta atttattttt atttctgcaa atgagtgacc cgaacgacgg
    32461 ccggcgcgct tttcttatcc agactgccgc taatgttgat catctggtcc ggctgaactt
    32521 ctcgtccatc aaagacggcc gcaggaataa cgacattaat ttcaccgctc ttatcgcgaa
    32581 aaacgtaacg gtcctctcct ttgtgagaaa tcaaattacc gcgtagtgaa accgaagcgc
    32641 catcgtgcat tttttttgcg aaatcaacgg tcattttttt tgcatcatcg gttccgcgat
    32701 agccatcttc tattgcatga ggcggcggtg gcgctgcatc ctgttttaat ccgccctggt
    32761 catctgccaa cgcataaggc atgacaagaa aacttgctaa tacaatggcc tgaaatttca
    32821 tactgactcc ttaattgcgt ttggtttgac tta
//
LOCUS       DAOTRK010000044        32586 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.044, whole genome shotgun sequence.
ACCESSION   DAOTRK010000044 DAOTRK010000000
VERSION     DAOTRK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.044"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(105..923)
                     /gene="cof"
                     /locus_tag="QIE41_001962"
     CDS             complement(105..923)
                     /gene="cof"
                     /locus_tag="QIE41_001962"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDS3530854.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGCVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            1023..2723
                     /locus_tag="QIE41_001963"
     CDS             1023..2723
                     /locus_tag="QIE41_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDS3530855.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTV
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2788..3483
                     /gene="queC"
                     /locus_tag="QIE41_001964"
     CDS             2788..3483
                     /gene="queC"
                     /locus_tag="QIE41_001964"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDS3530856.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(3535..3933)
                     /gene="fadM"
                     /locus_tag="QIE41_001965"
     CDS             complement(3535..3933)
                     /gene="fadM"
                     /locus_tag="QIE41_001965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDS3530857.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4039..4410)
                     /locus_tag="QIE41_001966"
     CDS             complement(4039..4410)
                     /locus_tag="QIE41_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDS3530858.1"
                     /translation="MKHGIKALIITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4561..6432)
                     /gene="ppiD"
                     /locus_tag="QIE41_001967"
     CDS             complement(4561..6432)
                     /gene="ppiD"
                     /locus_tag="QIE41_001967"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDS3530859.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLE
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEEQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6624..6896)
                     /gene="hupB"
                     /locus_tag="QIE41_001968"
     CDS             complement(6624..6896)
                     /gene="hupB"
                     /locus_tag="QIE41_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDS3530860.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7105..9459)
                     /gene="lon"
                     /locus_tag="QIE41_001969"
     CDS             complement(7105..9459)
                     /gene="lon"
                     /locus_tag="QIE41_001969"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDS3530861.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9647..10921)
                     /gene="clpX"
                     /locus_tag="QIE41_001970"
     CDS             complement(9647..10921)
                     /gene="clpX"
                     /locus_tag="QIE41_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDS3530862.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(11047..11670)
                     /gene="clpP"
                     /locus_tag="QIE41_001971"
     CDS             complement(11047..11670)
                     /gene="clpP"
                     /locus_tag="QIE41_001971"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDS3530863.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11917..13215)
                     /gene="tig"
                     /locus_tag="QIE41_001972"
     CDS             complement(11917..13215)
                     /gene="tig"
                     /locus_tag="QIE41_001972"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDS3530864.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13559..13876)
                     /gene="bolA"
                     /locus_tag="QIE41_001973"
     CDS             complement(13559..13876)
                     /gene="bolA"
                     /locus_tag="QIE41_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDS3530865.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14181..14759
                     /locus_tag="QIE41_001974"
     CDS             14181..14759
                     /locus_tag="QIE41_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDS3530866.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTMTLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNIEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14803..16278
                     /gene="ampG"
                     /locus_tag="QIE41_001975"
     CDS             14803..16278
                     /gene="ampG"
                     /locus_tag="QIE41_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDS3530867.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            16738..17685
                     /gene="cyoA"
                     /locus_tag="QIE41_001976"
     CDS             16738..17685
                     /gene="cyoA"
                     /locus_tag="QIE41_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDS3530868.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMNMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            17707..19698
                     /gene="cyoB"
                     /locus_tag="QIE41_001977"
     CDS             17707..19698
                     /gene="cyoB"
                     /locus_tag="QIE41_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDS3530869.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            19688..20302
                     /locus_tag="QIE41_001978"
     CDS             19688..20302
                     /locus_tag="QIE41_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDS3530870.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            20302..20631
                     /locus_tag="QIE41_001979"
     CDS             20302..20631
                     /locus_tag="QIE41_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDS3530871.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            20643..21533
                     /gene="cyoE"
                     /locus_tag="QIE41_001980"
     CDS             20643..21533
                     /gene="cyoE"
                     /locus_tag="QIE41_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDS3530872.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGRIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            21682..23046
                     /locus_tag="QIE41_001981"
     CDS             21682..23046
                     /locus_tag="QIE41_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3530873.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETAGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(23174..23665)
                     /gene="yajQ"
                     /locus_tag="QIE41_001982"
     CDS             complement(23174..23665)
                     /gene="yajQ"
                     /locus_tag="QIE41_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDS3530874.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            23833..24744
                     /gene="panE"
                     /locus_tag="QIE41_001983"
     CDS             23833..24744
                     /gene="panE"
                     /locus_tag="QIE41_001983"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDS3530875.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            24707..25297
                     /gene="yajL"
                     /locus_tag="QIE41_001984"
     CDS             24707..25297
                     /gene="yajL"
                     /locus_tag="QIE41_001984"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDS3530876.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETIKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(25351..26799)
                     /gene="thiI"
                     /locus_tag="QIE41_001985"
     CDS             complement(25351..26799)
                     /gene="thiI"
                     /locus_tag="QIE41_001985"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDS3530877.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            27005..27247
                     /gene="xseB"
                     /locus_tag="QIE41_001986"
     CDS             27005..27247
                     /gene="xseB"
                     /locus_tag="QIE41_001986"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDS3530878.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            27247..28146
                     /gene="ispA"
                     /locus_tag="QIE41_001987"
     CDS             27247..28146
                     /gene="ispA"
                     /locus_tag="QIE41_001987"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDS3530879.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            28171..30033
                     /gene="dxs"
                     /locus_tag="QIE41_001988"
     CDS             28171..30033
                     /gene="dxs"
                     /locus_tag="QIE41_001988"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDS3530880.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            30088..31062
                     /gene="yajO"
                     /locus_tag="QIE41_001989"
     CDS             30088..31062
                     /gene="yajO"
                     /locus_tag="QIE41_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDS3530881.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEGIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            31293..>32586
                     /locus_tag="QIE41_001990"
     CDS             31293..>32586
                     /locus_tag="QIE41_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3530882.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKHDLNQNLIERLWNKIRDFF
                     LGSDKQKAFKSIHKYINTLSVLNYNSASSPDPNFNIDASSDLDSYLNLKFDNLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKVGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDLGNCNCSNVNFEHTYFDSVKFTNT
                     NCTNANFNSCRFIKCDLTNMNCTGAILDNAVIYGKEKEPEMQYPEADQIIQRITYQKS
                     HGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKAEAESTEEKDISPLAKIILDYIESDKNPESLKFDEKSTTTKIIQDIDNF
                     IFYNQHLKSIFNQAMNLEGKISRKKYNEIFNYKQEQARQ"
BASE COUNT         7669 a         8467 c         8074 g         8376 t
ORIGIN      
        1 aataactggc tcaaagctga aagctttccg gaacccccag cctagctggg ggttttctgt
       61 gcacaaaaaa accgggcaat tgcccggtgc tggaagggat ctcgttattc gggggaataa
      121 ggtagatgtg gatagtccag ccagtgcgtc aaatagtgag agacagcctg atttcggcaa
      181 tgtccaatca ccggtaaatg cgggagctcc gcgcgcaatt gcggcatcgc attgcccata
      241 ataaatccgc taccgacgca gcctaacatt tcgcgatcgt tcatcgcatc accaaaggcc
      301 atgcaatcgc gcaacgataa acctaaatgt tgggtcagca ccgtcaatgc agcgccttta
      361 ttgcagccca ccggcagcac ttcgaggcaa tccgtagcgg aaaaacacaa atgtgcacgc
      421 tcgcctaatg cttcgtatag ctggatctgc aagcgtgtaa gatcgtcgtg atcgccacag
      481 aagcagatct tggtgacgct gccgagtggc atttttttga catcgattat ctgataacga
      541 aaaccgctat agacaaatgc ctgcaacaac gcagggatct ctttcccggt aaaccaaccg
      601 tcgtcattga agatatgcat gctggctcgg gtatcccatt gctgatacag caccagctcc
      661 gcgacatccg caggtaaatc atcacgatgt aaaagttcac cttccagaga atgcacgcgc
      721 gttccgttgc cggtaatcaa atatgcatcc agcgatagcg ccccgagaat atgctgcatc
      781 tccagcgcat gacgccccgt ggcaaaagtg agggtaatgt cgcgttcacg cagtcgcgcc
      841 aaagtagaga gggttttctc acctaaatga tggtcaggca tcaataaagt gccatccata
      901 tcaaatgctg ccagacgagc catttctttc tccactctgt gacacggtta atggtgattg
      961 agtatcacct gatatatacg gaagtaatag tgaatagtta aataatattg ttccgggttt
     1021 atatgcgact gctcaaccgt cttaaccagt atcaacgtct gtggcaacct tccgccggaa
     1081 agccgcaaac cgtcaccgtc agcgaactgg ccgaacgctg tttttgcagc gaacgccatg
     1141 ttcgtacgct gttacgccag gcacaggagg cgggatggct ggagtggcag gcgcagtcag
     1201 gacgcggaaa gcgcgggcaa ctacgctttc tggtcacgcc agaatcgcta cgcaatgcga
     1261 tgatggaaca ggcgctggaa accggaaagc agcaagatgt gctggagctg gcgcaactgg
     1321 ccccaggtga gctgcgcact ctgttacagc cgtttatggg cggacaatgg caaaacgata
     1381 cgcccacgtt gcgtattccc tactatcgcc cgctcgaacc gctacaaccg ggctttttgc
     1441 ccggccgtgc cgagcagcat ctcgccggac agatattttc cggcctgacc cgcttcgata
     1501 ataataccca gcgcccgatt ggcgatttag cgcatcactg ggaaacctct gctgacgggt
     1561 tacgctggga cttttatctt cgttcaaccc tacactggca taacggcgat gcagtaaaag
     1621 cctcacactt acaccagcga ttattgatgc tgttacaact gccagcactg gatcaattat
     1681 ttattagcgt gaagcgtatt gaagtcaccc atccgcagtg tctgaccttc tttttacatc
     1741 gccccgatta ctggcttgcg caccggctgg cgagctattg cagccatctg gcgcatccgc
     1801 aattccccct gatcggcact ggtccttttc gcttaacaca attcacagtg gaactggtgc
     1861 gcctggagag tcatgattat taccatttac gtcatccgct gcttaaagcg gttgagtact
     1921 ggataactcc gccgcttttc gaaaaagatt tgggaaccag ttgtcggcat cccgtgcaaa
     1981 tcactatcgg caaaccggag gagttgcaac gggtcagcca ggtcagtagt ggcatcagtt
     2041 taggtttttg ctatttaacg ttgcgcaaaa gtccccgact gtccctctgg caggcgcgaa
     2101 aagtgatctc cattattcat caatccggtt tattacaaac gttagaagtc ggagaaaacc
     2161 tgatcaccgc cagtcatgca ttactgccag gctggactat tccgcaatgg caagtaccgg
     2221 atgaggtcaa actaccgaaa accttgacac tggtttatca cctaccgata gaacttcata
     2281 ccatggcaga acgtctacag gcgacactgg cagcggaagg ctgtgaactc acaattattt
     2341 ttcataacgc aaaaaactgg gacgacacga ccctactggc acacgcagac ctcatgatgg
     2401 gcgacagatt aattggcgaa gcaccggaat ataccctgga gcaatggctg cgttgcgatc
     2461 cactatggcc acatgttttc gacgctccag catatgcaca tttgcaatcg acactggacg
     2521 cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc cctaaaagcg gtttttagcc
     2581 agttaatggc agacgcgacg ctgacgccgc tattcaacta tcactatcgc attagtgccc
     2641 ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg gtttgaattt accgaagcct
     2701 ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta atcgcaggcg ttaccataac
     2761 gcttttattg ttcaagcagg attatctatg aaacgtgctg tcgttgtgtt cagtggaggc
     2821 caggattcca ccacctgtct ggtgcaggca ttacaacaat atgatgaagt ccattgcgtg
     2881 acgttcgatt acggtcagcg gcatcgcgca gaaatcgacg tggcacgcga actggcgctg
     2941 aaactggggg cacgcgcgca taaggtgctg gatgtcaccc tgctcaacga gctggcggtc
     3001 agtagcctga cgcgtgacag cattccggtg cctgattatg aacctgaagc tgatggcatc
     3061 ccgaatacgt ttgtccctgg gcgtaatatt ttgttcctga cgctggcggc aatatatgcg
     3121 tatcaggtaa aagcagaagc ggtgattacc ggcgtctgtg aaacggattt ctccggctac
     3181 ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg ccgtcagttt gggcatggcg
     3241 aaagatattc gttttgaaac gccgctgatg tggattgata aagcggaaac ctgggcgctg
     3301 gcagattatt acggcaaact ggatttagtc cgtaacgaaa cgttgacctg ctataacggc
     3361 attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt tacgcgccaa cggtttgaat
     3421 cattatctgg ccgataaacc gacggtgatg gcagcgatga agcagaaaac cgggttgagg
     3481 taattattcc gggtgtcgcc ggatgcggct tgagcatccg gcaccacaaa acgtttactt
     3541 aaccatctgc tcgagctttt cgcgcaattc cccttccaga gctaacgctt tctgcgtttt
     3601 aagatcaata caaacaaacg taataagcgc atccgctacc acctgccctt ccggctccag
     3661 tgtaatgacc tggcttaaga tgccgctttt accgtttaat tgctgcaact gactggtaat
     3721 ggttaacagg tcgcttaata ccgccgggcg acgatagtta atattgatat tgaccacgac
     3781 gaaggcgatg ttatgggccg tcatccactg aaaactgtcg ctattttcca acccatccca
     3841 gcgggcttct tcgagaaact ccagatagcg agcgttgttt acgtgctggt aaacgtcgag
     3901 atgatatcca cgaactttga tttgtgtttg catagcgcaa taaccttacg ttgtttttat
     3961 aaaaactgtt ataagtacag aggccataac tggtatgacc tcttcagtct ggcaaaaaat
     4021 tgccactatg caaattaatt acagggttaa taccgccaga ttacgttcca ccagcgaatt
     4081 gcccatccct ggcacctgct tgagatcctc caccgtttta aacggaccgt actcttcgcg
     4141 ataactgaca atcgcctgcg ctttcttcag gccaacgcca ttcatcgcgc gggctagctc
     4201 ttccgcgctg gcattattaa tgctgacccg agtgccttct tcgtcactgg ctttcgccgg
     4261 tactgctgct ttactttgtg ctgcaggagc ttccgctttg gtttctaccg ttgtcggttt
     4321 cgccgcagga gctgccgcca gcgcgctatg agacattccg gcacaggcca gagacagggt
     4381 aatgatcagt gctttaattc cgtgtttcat actgttttct ccttgtttgt taacagtgga
     4441 atcaccttaa cggcagtgaa aacagtaaac aaatggcaat gttcagaaat ggaaaaggcc
     4501 gcgaaagcga ccttttcttg ttacagctgc aaagcatttt tttgcgaggc ggcttcagga
     4561 ttattgctgt tccagcgcat cgccaatttt gattttcgcc tctttacgca ggttactcat
     4621 cagagcttca aagacgattt gtgcgttgtt ctgggtgata ccctgcacca tcgctttttt
     4681 ctgttcttcc ggcattgaac cttgtttcac ttcatccagc gccagcagaa ccacattacc
     4741 ttgcatatcg gtcgccatac cgtagctcgg tttgtctttc gctggcagtg gcagtgcaaa
     4801 cgccgcctgg ctaatcgggt cacgaccgga acggcttaag gttttcggct cgccaaattt
     4861 cagaccggca gcctgcatag cttccgcacc tttgccggct ttcaaatcaa ccagcagttt
     4921 ctcagcatcc actttcgcct gttgttcagc tttgttatgc tgaaccagtg ccttaacttg
     4981 ttcctgaaca tctgccaacg gtttcaccgc ttccggtttg tgctcgctga tgcgcagcac
     5041 gaatgcgcgg tcgccatcga cggtgatgat gtcagagttg atgcccggcg cgccgttttc
     5101 acctaccaga ccgccgttga agatagcgtc agcaaccggc ttgaagttca actcttccgg
     5161 caggttatct ttgctgaacc accccgtctg agtggcttta acgccggctg cttgctctgc
     5221 accggccaga gactcggtgt cgttgcttgc cgcatcgctc actttctgtt gcagtgcgta
     5281 gtacgcatcg agggcttttt cgtgtttcac tttcgccgcg acatcgtcac gcacttcgtc
     5341 taacgacttc actttcgctg gctgaatgtc gtccagacgt acaatcagga aaccgaccga
     5401 agatttgatg acaccagaca gttggccttt ttctttcagg ccagcatttt ttagttcgtc
     5461 cgggatagtg gcatcttcta accaacccat atcgccgccg ttacgggcag agataatatc
     5521 ggcagatttt tctttggcta acgcagcaaa atcaccgcct ttattcagct catcaagtac
     5581 cgctttcgct tcatcttcag ttttggtctg gatgatgctg tagcgggtac gctgcggctg
     5641 ggtgaattga tcctgatgct gatcgtagta gctctggata tccgcatcgc taaccggttg
     5701 ctgcatcgtt gcggcatcca gcttgatgta actcacgcgg aattgttccg gcgtcatgaa
     5761 attgtttttg ttttgttcgt agtagctggc aatttcctgt tcggtcacag gctgcttcgc
     5821 cgccagcgcg ttaacatcga tagtcgcttc acgcaccacg cgttgttgag ctaccagtgc
     5881 cgccagctcg tcggtttcac ctttcagcat aaaatcggta ccggcaacgc cgttaatcag
     5941 ctgttgggta gtgagctggt tacgcagcgc ctgggcgtac tgatcggcgg tcatccccat
     6001 ctggttgagg ataccgttat agcggctgtt atcaaatttg ccgtcaacct ggaaggctgg
     6061 ggtcgcgaaa atcgcctgtt taacctgctc atcgctgata cccagtttca gctcacgagc
     6121 gtactgctcc agcagcgcct cgtcgatcag acgattcagc acctgttgac gcagggtttt
     6181 catatagcct tcgttcgctg caagctcaga gtattgatcg cccagctgtt gctgcatgcg
     6241 attacgctcg ctgttgaagg cgttctcgaa ttgcccacgg ctgatttcct ggtcattcac
     6301 ttttgcggcg tagttattgc ctccgccaat caggtaacca ctcacgccgg tcaatatgaa
     6361 cgacacgata atgataccga aaataatctt gagcacgaga ctgtttgcag ccgtgcgtaa
     6421 gctgtccatc atggtgtaac aacactccgc tgtagatgac tgtgtacctc acgcaacgta
     6481 tcgatgcgcg caaaggccta ttgtgacaag aaacgggggc aattgtcagc ccacaagacg
     6541 cagaaagtca ccgaatacaa ataaaaaagg cacatcagta gatgcgccct tgaacttcgt
     6601 cacatcccca ctggggacaa cgcttagttt accgcgtctt tcagtgcttt acctgcacgg
     6661 aagctcggta ctttagcggc agcgatggtg atctctttac cggtctgcgg gttgcggcca
     6721 gtacgggcag cacgctcttt aacggcaaaa gtaccaaaac ctaccagtgc tacatcatcc
     6781 ccttctttca gagattcagt tacggaagca ataatagcat ctaacgcacg gccagccgca
     6841 gctttagaga tatcagcccc tgcagcaatc ttgtcgatca attgagattt attcactctt
     6901 ctcttcctct ttataattta tatcgcactt gaatccttca aggtacgaac gcgcagcagt
     6961 tatatcaggc cagccatccc cttacaacac cccttaataa gggcaagccc gatccgccat
     7021 ctaacttagc gagacaaaaa aaggctggca agcccgaatt agcctgccag ccctgttttt
     7081 attagtgcat tttgcgcgag gtcactattt tgcagtcaca acctgcatgc cagacggttc
     7141 attttgcagc gccagagtca gaacttcctc aatgcgtttc acaggatgaa tgtccagatc
     7201 ggcaattacg ttgtcaggaa tctcttccag atcgcgttta ttttcgaacg gaattaacac
     7261 tgttttaatc ccgccgcgat gcgctgccag tagtttttct ttcaaaccac cgataggcag
     7321 tacctgacca cgcagagtga tctcaccggt cattgccaca tcggcacgaa ccgggttacc
     7381 ggtcaggcaa gaaaccagcg cggtgcacat agcaataccg gcactcggac catctttcgg
     7441 cgtcgcacct tccggtacgt ggacgtggat gtcgcgtttt tcgtaaaaat cagggttgat
     7501 ccccagtttt tccgcacgcg cacgaaccac cgttaacgcc gcctgaatgg actcctgcat
     7561 cacttcaccg agcgaaccgg tataggtcag tttgcctttc cccggaacac atgcggtttc
     7621 aatggtcagc aagtcaccgc ccacttccgt ccacgccaga ccggttacct gaccgacacg
     7681 gttttcgtta tcagcgcgtc catagtcgaa acgctgaaca ccgaggtagt catgcaggtt
     7741 atcaccgtta atttcgatat gttttaatga cttatcgagc agtaactgct taaccgcttt
     7801 gcgacacagt ttggagattt cacgctccag accacgcacg cccgcctcac gggtgtaata
     7861 acgaataatg ccgataatgg cgctatcgtc gacggtcagc tcaccttttt tcagtgcatt
     7921 acgttcaatc tgcttcggca gcaggtgacg tttggcgatg ttcagttttt catcttcggt
     7981 ataaccggag aggcgaatca cttccatacg atccagcagc ggtgccggaa tgttcatgga
     8041 gttcgacgtc gcgacaaaca tcacgtcgct gagatcgtaa tccacttcca ggtagtggtc
     8101 gctgaacgct acgttctgct ctggatccag cacttcaagc agtgcggagg ccggatcgcc
     8161 acgcatgtca gaagacattt tgtcgatctc atcaagcagg aacagcgggt ttttcacgcc
     8221 cactttcgcc attttctgga tcagtttacc cggcatagaa ccgatgtaag tacggcggtg
     8281 accacggatc tccgcttcat cacgcacgcc gcccagcgcc atacggacat atttacgccc
     8341 ggttgctttg gcgatggact gacccaggga ggttttacct acccccggcg gccctaccag
     8401 gcagaggatc ggtcccttga ttttgttgac acggctttga accgcaagat actcaaggat
     8461 gcgatctttc acgcgctcca gaccataatg gtcggtatca aggatttcct gcgcctgacg
     8521 caggtctttt ttgaccttgc tacgcgcatt ccacggcacc tgtaccatcc agtcgatata
     8581 accacgcact acggtcgctt ctgccgacat cggagacatc attttcagct tctgcaactc
     8641 tgcttccgct ttctcttttg cctctttcgg cattttcgcc gcgtcgattt tgcgcttcag
     8701 ggcttcgttt tcgtccggcg cgtcgtccat ttcaccgagt tctttctgaa tagctttcat
     8761 ttgctcgttc agatagtact cacgctggga tttctccatc tgctttttaa cgcggttgcg
     8821 aatgcgtttc tcaacctgca gcagatcgat ttccgattcc atcattgcca tcagatattc
     8881 cagacgttcg ttaacgtcgg acatctccag aacggactgt ttgtcagcca gtttcagcgg
     8941 catatgtgca gcaatggtat ccgccagacg cgccgggtcg tcgatgctat tcagcgacgt
     9001 cagcacttct ggtgggattt ttttgttcag cttgatgtag ccttcgaact ggctgattgc
     9061 agtacgcacc agcacttcct gttcccgctc atcaatggtc ggcgactcca gatactccgc
     9121 cttcgcagaa aagtgttcgc cattgtcaga gagcgcagaa atacgcgcgc gctgtaaccc
     9181 ctcgaccagc actttgacgg tgccgtcagg cagtttcagc atctgcaata tagaggccac
     9241 ggtcccgacg gtgaaaagat cgtttacacc cggctcatcc gttgaagctt ctttctgcgc
     9301 gaccagcata atttttttat catggtccat cgccgcttcc agacaacgga tagatttttc
     9361 ccgcccgaca aataagggga tgaccatgtg cggataaacc accacatcgc gcagcggcaa
     9421 tacggggatt tcaatgcgtt cagaacgctc aggattcata gagctctctc ttagtttaat
     9481 ttccgccagg taatcagatg acacgactgt gcttcacgcc atctattaac atgtacgtca
     9541 gtatatgggg atgtttcccc cacattcaac gccgagaata gaggaaaaat taaaggggag
     9601 ataaaatccc ccctttttgg ttaactaatt gtatgggaat ggttaattat tcaccagatg
     9661 cctgttgcgc ttccggcttg ccataaatca gcaacggctt gctttgacca tcaattaccg
     9721 attcgtcgat aaccactttt tcgacgtctt ccatggacgg cagatcgtac atggtatcga
     9781 gcagtgcggc ttctacgatg gaacgcaggc cacgggcacc ggttttacgc gccatcgctt
     9841 tcttagcgat agcatccagc gcctcgtcac ggaattccag atccacgcct tccagattaa
     9901 acagcgcctg atactgcttg gtcagggcgt ttttcggctc tttgaggatc tgaatcagag
     9961 cttcttcgct cagttcattc aacgttgcga caaccggcag acgaccaata aactcaggga
    10021 taagaccaaa cttgatcaga tcttccggtt caacctgcgc cagcagctcg ccttcgcttg
    10081 ctttgtcgga cttcgctttt accgtcgcgc caaaaccaat gccggagccg gtttctacac
    10141 gatgggaaat cactttatcc agaccggcaa acgcaccgcc acaaataaac aggatcttag
    10201 aggtatcaac ctgcaagaat tcctgctgcg gatgtttacg cccaccttgc ggcggaacag
    10261 cagctaccgt accttcgatc agtttcaaca gtgcctgctg tacgccttca ccggaaacgt
    10321 ctcgggtaat ggacgggttg tctgacttac gagaaatctt gtcgatttca tcgatgtaga
    10381 caatcccgcg ctgcgctttc tgtacgtcgt aatcgcactt ctgcaacagc ttctgaatga
    10441 tgttttcaac gtcttcgccc acataaccgg cttcggtcag cgtggttgcg tcggccatgg
    10501 tgaacgggac gtccagcagg cgcgccagcg tttcggccag cagcgtttta ccggaaccgg
    10561 tcggaccgat cagcagaatg ttacttttgc ccaactcgac accattgctg gtatcgccgt
    10621 tgcgcagacg tttgtagtgg ttgtataccg cgaccgccag cacttttttc gcctgttcct
    10681 ggccgataac gtaatcgtcc aggtggttac gaatttcatg cggcgtcggt agcgcactgc
    10741 gttcacgatg cggtgcaact tctttaatct cttcgcgaat gatgtcgtta cataaatcaa
    10801 cacattcgtc gcagatatac acggatggac cggcaatcag cttgcgcact tcatgctggc
    10861 ttttgccgca aaaagagcaa tacagcaatt tgcctgagcc atctttgcgt ttatctgtca
    10921 tgggtcaaaa cctcttcttt gttctttgtg ccgcacacga cgacgcaaat gccattctca
    10981 ggcgcaagcc gcttacagcg ttgtgccgcc ctggataagt atagcggcac agttgcgcct
    11041 ctggcatcaa ttacgatggg tcagaatcga atcgaccaga ccgtattcca ccgcttcagg
    11101 ggcggaaagg aagcgatcgc gctcggtatc acgttcaatc tgttctaatg attgacccgt
    11161 atgaagcgcc ataagttcat tcatgcgtcc tttaactttc agaatttcac gggcatgaat
    11221 ttcgatatcg gtcgcctggc cctggtagcc gcccaacggc tggtgaatca tcacgcgcga
    11281 attcggcagg cagaaacgtt taccttttgc ccctgcagtc agcaagaaag cgcccatcga
    11341 agccgcctgg cccatacaga tggtgctgac atcaggcttg ataaactgca tggtgtcata
    11401 gatagacatc ccggcagtaa tcaccccgcc tggggagttg atgtacagat agatatcttt
    11461 ttctgggttt tctgcttcca ggaacagcat ctgcgccaca atcaggttag ccatgtggtc
    11521 ttcaacctgg ccagtcagaa aaatgacgcg ttccttaagt agacgagaat agatatcaaa
    11581 agagcgctca ccacgtgagg tctgttcaat gaccatcggc accagcgcca tatggggtgc
    11641 aaagttatct cgttcgccgc tgtatgacat ttccgtctcc tggataaaat tgaaaaaacc
    11701 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    11761 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    11821 ttcgcacggt tccatgcaaa attcatgaca aaaaaaaagc ccaccacctg gcggtgacgg
    11881 gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt tcatcagctc
    11941 gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt caacagcctg
    12001 ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc tgtagaactc
    12061 gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc ctttcacgcg
    12121 ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca ggccaacaac
    12181 tacgcgacgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag cttgtttttc
    12241 gttgccaccg aaacgctgtg cagcctgacg acgcagaacg tcgatttcac tgtcgatcag
    12301 cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct gagacttaac
    12361 gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt cagcgcgcag
    12421 accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag tcagttccgg
    12481 cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt tacctttcag
    12541 gttttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt cgccagcttt
    12601 gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca tcgccagtac
    12661 gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg tgaagtcgat
    12721 ggttacgcgg tcttctgctt caacagcgcc gtctttttct ttccaggtcg cctgctgttt
    12781 acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga tcggtttttc
    12841 aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa actctacaga
    12901 gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg cgccagccgg
    12961 attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt cacccagaac
    13021 gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca ctttgccttt
    13081 gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt taacagcggt
    13141 ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt gagtggtttc
    13201 aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac tcttaccccg
    13261 caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc acattccctg
    13321 ttaccagaat catcccgaaa gcaatcaaat aggacgcggc attatagcgg catcactttt
    13381 atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc cgggcaattt
    13441 tcgtctgaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc ccgcaggccg
    13501 tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa atgcgttttt
    13561 acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc tgcaaccctt
    13621 cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta gagagttcct
    13681 ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc gtaaaacgat
    13741 cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg tgacgatagc
    13801 tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt tcttctatcc
    13861 gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc catcccttta
    13921 aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta ggtttcgtcc
    13981 tggtttttct gtcaagcgta tgaagtttac tgatttaaag attgaaacac aggcctcgca
    14041 tcagcgtttc ttttaccagg ggcgattatt tcgtcaacat gatgaattta catgccttac
    14101 ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa acacgcttaa
    14161 gagtctctga gagtccgaac atgttcaaaa aaatcctctt cccgttagtt gctctgttta
    14221 tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg atgacactgc
    14281 cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc gatcagcgta
    14341 ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg accgcctccc
    14401 gcgatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg cgtggttaca
    14461 tggtcggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg tatgctgatg
    14521 tgtcccaggg caacgtgcgc tataacatcg cgaccaaagc ggatatcgcc atcatcgcta
    14581 ccgcgcagaa tggcaacaaa atgaccaaga actatcgtgc cagctacaac attgaaggtg
    14641 cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg accgatacca
    14701 tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac gcgcgttaat
    14761 ttctgccctc tggcccggtg caagccgggc ctgtagacgc ccatgtccag tcaatattta
    14821 cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc ttccgggcta
    14881 ccgctcgccc tgacatccgg caccttacag gcctggatga cggtcgagaa tatcgacctc
    14941 aaaaccattg gtttcttctc gctggtaggc caggcttacg tttttaaatt cctctggtca
    15001 ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg gctgctcgcc
    15061 acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc tggcacccaa
    15121 ctccgctgga tggcggcact ggcagtggtg atcgcttttt gctctgcctc ccaggatatt
    15181 gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggcgc gggcgcggca
    15241 atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc cctatggctg
    15301 gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct gttgatcccc
    15361 tgtattatcg cgacgttgct tgcaccagaa ccaaccgaca ccattcctgt gccaaaaacg
    15421 ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa tgcctggctt
    15481 attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag cctgacaacc
    15541 acgtttttga ttcgtggcgt cgggtttgat gcgggtgaag taggcgtggt taacaaaacg
    15601 cttggcttac tggcgaccat tgttggcgca ttgtacggtg ggattttgat gcagcgcctg
    15661 tcactgttcc gggcgctgct aattttcggt attttacaag gtgcgtctaa cgccggttac
    15721 tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt ctttttcgaa
    15781 aacctctgtg gcgggatggg tacatcagcc tttgtcgcgc tgttaatgac gctgtgtaat
    15841 aagtcatttt ccgctactca atttgccctg ctctcagcgc tttctgctgt agggcgagtt
    15901 tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac attctatctg
    15961 ttctccgtcg ccgctgccgt accagggctt attttgctgc tggtttgccg ccagacgctt
    16021 gaatatacac gagtaaatga caactttatc tcccgtaccg aatatcctgc aggttatgcc
    16081 tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt actgctgttg
    16141 acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct ggaagtcggg
    16201 gttttagtcg ccctttctgg cgtcgtgctt ggtggtttac tggattatct ggcgctacga
    16261 aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact cgccgtaatt
    16321 attacggcta aataaacatc agcatcgctt attaatattt gttcttttgt gcggcttagc
    16381 gtctggaaat tattgacgcc atttatgaat tctatttttc ttacacgatt cagctaatga
    16441 gtctttattt tctcactacc cagttgtcac cttaatgata attatttgtt aaataattgt
    16501 tttatttcgc attggttata ccaattgccc gcccagactc cttctgcact ccccttttgt
    16561 tataacgccc ttttgcaaca gcttcttaaa atcaacctga tatgttttgc aacatatgtg
    16621 acctggaagc caaatccaag taacaggaat ttaatcatgt ttacagtaat ataaccttcc
    16681 cgtaaaatgc ccacacactt taaacgccac cagatcccgt ggaattgagg tcgttaaatg
    16741 agactcagga aatacaataa aagtttggga tggttgtcat tatttgcagg cactgtattg
    16801 ctcagtggct gtaattctgc gctgttagat cccaaaggac agattggtct ggagcaacgt
    16861 tcactgatac tgacggcatt tggcctgatg ttgattgtcg ttattcccgc aatcttgatg
    16921 gctgttggtt tcgcctggaa gtaccgtgcg agcaataaag atgctaagta cagcccgaac
    16981 tggtcacact ccaataaagt ggaagctgtg gtctggacgg tacctatctt aatcatcatc
    17041 ttccttgcgg tactgacctg gaaaaccact cacgctcttg agcctagcaa gccgctggca
    17101 cacgacgaga agcccattac catcgaagtg gtttccatgg actggaaatg gttcttcatc
    17161 tacccggaac agggcattgc taccgtgaat gaaatcgctt tcccggcgaa cactccggtg
    17221 tacttcaaag tgacctccaa ctccgtgatg aactccttct tcattccgcg tttgggtagc
    17281 cagatttatg ccatggccgg tatgcagact cgcctgcatc tgatcgccaa cgaacccggt
    17341 acttatgacg gtatctccgc cagctacagc ggcccgggct tctcaggcat gaagttcaaa
    17401 gctattgcaa caccggatcg cgccgcattc gaccagtggg tcgcaaaagc gaaacagtcg
    17461 ccgaacacca tgtctgacat ggcagcgttc gaaaaactgg ccgcgcctag cgaatacaac
    17521 caggtggaat atttctccaa cgtgaaacca gacttgtttg ccgatgtgat taacaagttt
    17581 atggctcacg gtaagagcat gaacatgacc cagccagaag gtgagcacag cgcacacgaa
    17641 ggtatggaag gcatggacat gagccacgcg gaatccgccc attaaagggg ttgaggaaga
    17701 ataaagatgt tcggaaaatt atcacttgat gcagtcccgt tccatgaacc tatcgtcatg
    17761 gttacgatcg ctggcattat tttgggaggt ctggcgctcg ttggcctgat cacttacttc
    17821 ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg tcgaccataa acgcctcggt
    17881 atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg gttttgctga cgccattatg
    17941 atgcgtagcc agcaggctct tgcctcggcg ggcgaagcgg gcttcctgcc acctcaccac
    18001 tacgatcaga tcttcaccgc gcacggcgtg attatgatct tcttcgtggc gatgcctttc
    18061 gttatcggtc tgatgaacct ggtggttccg ctgcagatcg gcgcgcgtga cgttgcgttc
    18121 ccgttcctca acaacctaag cttctggttt accgttgttg gtgtgattct ggttaacgtt
    18181 tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg cctatccacc gctatcggga
    18241 atagagtaca gtccgggagt cggtgtcgat tactggatat ggagtctcca gctatccggt
    18301 ataggtacga cgctaaccgg tatcaacttc ttcgttacca ttctgaagat gcgcgcaccg
    18361 ggcatgacca tgttcaagat gccagtattt acctgggcat cactgtgcgc gaacgttctg
    18421 attattgctt ccttcccaat tctgacggtt accgtcgcgt tgttgaccct ggatcgctat
    18481 ctgggcaccc atttctttac caacgatatg ggtggcaaca tgatgatgta catcaacctg
    18541 atttgggcct ggggccaccc ggaagtttac atcctgatcc tgcctgtttt cggtgtgttc
    18601 tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg gttatacctc gctggtatgg
    18661 gcaaccgtct gtatcaccgt gctgtcgttc atcgtctggc tgcaccactt ctttacgatg
    18721 ggtgcgggcg cgaacgtaaa cgccttcttt ggtatcacca ccatgattat cgccatcccg
    18781 accggggtga agatcttcaa ctggctgttc accatgtatc agggccgcat cgtgttccat
    18841 tctgcgatgc tgtggactat cggttttatc gtcaccttct cggtgggcgg gatgactggc
    18901 gtgctgctgg cagtaccggg cgcggacttc gttctgcata acagcctgtt cctgattgcg
    18961 cacttccata acgtgatcat cggcggcgtg gtcttcggct gcttcgcagg gatgacctac
    19021 tggtggccta aagcgttcgg tttcaaactg aacgaaacct ggggtaaacg cgcgttctgg
    19081 ttctggatca tcggcttctt cgttgccttt atgccgctgt atgcgctggg cttcatgggc
    19141 atgacccgtc gtttgagcca gcagattgac ccgcagttcc acaccatgct gatgattgca
    19201 gccagcggtg cagtactgat tgcgctgggt attctctgcc tcgttattca gatgtacgtt
    19261 tctattcgtg accgcgacca gaaccgtgac ctgactggcg acccgtgggg tggccgtacg
    19321 ctggagtggg caacctcttc cccgcctccg ttctataact ttgccgttgt gccgcacgtt
    19381 cacgagcgtg atgcattctg ggaaatgaaa gagaaaggcg aagcgtacaa aaagcctgac
    19441 cactatgaag aaattcatat gccgaaaaac agcggtgcag gtatcgtcat tgcagctttc
    19501 tccaccatct tcggtttcgc catgatctgg catatctggt ggctggcgat tgttggcttc
    19561 gcaggcatga tcatcacctg gatcgtgaaa agcttcgacg aggacgtgga ttactacgtg
    19621 ccggtggcag aaatcgaaaa actggaaaac cagcatttcg atgagattac taaggcaggg
    19681 ctgaaaaatg gcaactgata ctttgacgca cgcgactgcc cacgcgcacg aacacgggca
    19741 ccacgatgca ggcggaacca aaatcttcgg attttggatc tacctgatga gcgactgcat
    19801 tctgttctct atcttgtttg ctacctatgc cgttctggtt aacggcaccg caggcggccc
    19861 gacaggtaag gacattttcg aactgccgtt cgttctggtt gaaactttct tgctgttgtt
    19921 cagctccatc acctacggca tggcggctat cgccatgtac aaaaacaaca aaagccaggt
    19981 tatctcctgg ctggcgttga cctggttgtt tggtgccgga tttatcggga tggaaatcta
    20041 tgaattccat cacctgattg ttaacggcat gggtccggat cgcagcggct tcctgtcagc
    20101 gttctttgcg ttggtcggca cgcacggtct gcacgtcact tccggtctta tctggatggc
    20161 ggtgttgatg gtgcaaatcg cccgtcgcgg cctgaccagc actaaccgta cccgcatcat
    20221 gtgcctgagc ctgttctggc acttcctgga tgtggtttgg atctgtgtgt tcactgttgt
    20281 ttatctgatg ggggcgatgt aatgagtcat tctaccgatc acagcggcgc gtcccatggc
    20341 agcgtaaaaa cctacatgac aggctttatc ctgtcgatca ttctgacggt gattccgttc
    20401 tggatggtga tgacaggggc tgcctctccg gccgtaattc tgggaacaat cctggcaatg
    20461 gcagtggtac aggttctggt gcatctggtg tgcttcctgc acatgaatac caaatcagat
    20521 gaaggctgga acatgacggc gtttgtcttc accgtgctaa tcatcgctat cctggttgta
    20581 ggctccatct ggattatgtg gaaccttaac tacaacatga tgatgcacta agagcggcgg
    20641 ttatgatgtt taagcaatac ctgcaagtaa cgaaaccagg catcatcttt ggcaacctga
    20701 tctcggtgat tgggggattc ctgctggcct caaagggccg cattgattat cccctgttta
    20761 tctacacgct ggttggggtg tcactggttg tggcgtcggg ttgtgtgttt aacaactaca
    20821 tcgacaggga tatcgacaga aagatggaaa ggacgaagaa tcgggtgctg gttaaaggcc
    20881 tgatctctcc tgctgtctcg ctggtgtacg ccaccttgct gggtattgct ggctttatgc
    20941 tgctgtggtt tggcgcgaat ccgctggcct gctggctggg ggtgatgggc tttgtggttt
    21001 acgtcggcgt ttatagcctg tacatgaaac gccactcagt ctacggcaca ttgattggtt
    21061 cgctctccgg cgctgcgccg ccggtgatcg gctactgtgc agtaaccggc gagttcgata
    21121 gtggcgcagc gatcctgctt gctatcttca gtctgtggca gatgcctcac tcctatgcca
    21181 tcgccatttt ccgctttaag gattaccagg cggcaaacat tccggtattg ccagtggtaa
    21241 aaggcatttc ggtggcgaag aatcacatca cgctgtatat catcgccttt gccgttgcca
    21301 cgctgatgct ctctcttggc ggttacgctg ggtataaata tctggtagtc gccgcggcgg
    21361 ttagcgtctg gtggttaggt atggccctgc gcggttataa agttgctgat gacagaatct
    21421 gggcgcgcaa gctgttcggc ttctctatca tcgccatcac tgccctctcg gtgatgatgt
    21481 ccgttgattt tatggtgccg gattcgcata cgctgctggc tgctgtgtgg taatacaacc
    21541 tctcagttaa aaaggtgctg cggcaccttt tttcttagca ttagaaacat atccctctcg
    21601 aaatatttac taaaaaatcc gtatgtttac cccattcgtt tgccgcttta cactagtcgc
    21661 gaatttaaaa cagaggtggt aatgaacgat tataaaatga cgccaggtga gcggcgcgcg
    21721 acctggggtt tagggaccgt attctcgttg cgcatgctgg gcatgtttat ggttctgccg
    21781 gttctgacca cgtacggcat ggctctgcaa ggtgccagcg aagcattaat cggtattgcc
    21841 attggtattt atggtctgac tcaggccgtt tttcagattc cgtttggcct gctttccgac
    21901 cgcattggtc gcaaaccatt aattgtcggt gggctggcag tgtttgccgc cggtagcgtt
    21961 atcgctgcgc tctctgactc catctgggga attattctgg gccgggcgct acaaggctcc
    22021 ggtgcgattg ccgctgccgt tatggcgctg ctttccgatc tcacgcgcga acaaaaccgc
    22081 accaaagcaa tggcgtttat cggcgtgagc tttggcatta cctttgccat tgcgatggtg
    22141 cttggcccga tcatcactca caaacttggg ctgcacgcgc tgttctggat gatcgctatt
    22201 ctggcaacga ccggcattgc gttgaccatt tgggttgtgc ccaacagtag cactcacgta
    22261 cttaatcgtg agtccggaat ggtgaaaggc agtttcagta aagtgctggc ggaaccgcgg
    22321 ctgctgaaac tcaactttgg cattatgtgt ctgcatattt tgctgatgtc gacgtttgtt
    22381 gccctgcccg gacaactggc tgatgcgggg ttcccggcgg ctgaacactg gaaggtctat
    22441 ctggcgacaa tgctaatcgc ctttggctcg gtcgtgcctt tcattatcta cgctgaagtt
    22501 aagcgcaaaa tgaagcaagt ctttgtcttc tgcgttgggt tgatcgtggt tgcggaaatt
    22561 gtgttgtgga acgcacaaac gcagttctgg caactggtgg tcggcgtgca gcttttcttt
    22621 gtagcgttta atttgatgga agcccttctg ccttcactta tcagtaaaga gtcgccagca
    22681 ggttacaaag gtacggcgat gggtgtttac tccaccagcc agtttcttgg cgtggcgatt
    22741 ggcggttcgc tgggcggctg gattgacggc atgtttgacg gtcagggagt atttctcgct
    22801 ggcgcaatgc tggccgcagt gtggctggca gtcgccagta ccatgaaaga accgccgtat
    22861 gtcagcagct tgcgcattga aatcccggcg gacattgccg caaacgaggc gttaaaagtg
    22921 cgtttgctgg aaaccgcggg cgtcaaagaa gtgttgattg cagaagaaga acattcagct
    22981 tatgtgaaaa tcgacagcaa agtgacgaat cgctttgagg ttgaacaggc aattcgtcag
    23041 gcataaaaaa cgaggcgaat tgcctgctgc gctacgctta ccaggcctac aaagttcctg
    23101 agttacaaat cgttgtaggc cgaattagct gttcatgctg cattcggcat gaacaaaacg
    23161 cacattgtcg cgattaatcg cggaagtttt tgaactggaa cggctgaccg agatcgccac
    23221 cacgtaccat cgccatgaca gcctgcaaat catcacgaga tttacccgtt acgcggatct
    23281 catcaccctg aatttgcgcc tgcactttca gtttgctgtc tttgatcatc ttgacgattt
    23341 tcttctgggt cgcgctctca atgccctgtt tcagtttcgc ttccacaaac caggttttac
    23401 cgctatgaac gatattttcc ggtacatcca gtgaactgcc ttcaatgccg cgcttcagca
    23461 gcttggcacg cagaatatcc agcaactgat tgacctggaa atcggactcg ctcaacactt
    23521 tgatggtttt gctggcgtcg ttcagctcaa atgaggcttc aacattacgg aagtcaaaac
    23581 gggactccac ttcgcggctc gcgttatcga ccgcgttacg tgcttcctga agatcaactt
    23641 cagagacaat atcgaaagat ggcatctttt cctctccctt catttttgat gcgaagcata
    23701 atacctgcaa agttaaataa ccaacagtcc tgcaccagga cgtatccctt aattgtgcat
    23761 agtcgcaaca gcatgcacaa ttaagggata gccttattga acacccggag tggttgcggg
    23821 tgaggaggaa caatgaaaat taccgtattg ggatgcggtg ccttagggca attatggctt
    23881 acagcacttt gtaaacaggg tcacgaggtt cagggctggc tgcgcgtacc acaaccttat
    23941 tgtagtgtga atctggttga gacagatggt tcgatattta acgaatcgct gaccgccaac
    24001 gatcccgatt ttctcgccac cagcgatctg ctcctggtga cgctgaaagc atggcaggtt
    24061 tccgatgccg tcaaaagcct cgcgtccaca ctgcctgtaa ctacgccaat actgttaatt
    24121 cacaacggca tgggcaccat cgaagagttg caaaacattc agcagccatt actgatgggc
    24181 accaccaccc atgccgcccg ccgcgacggc aatgtcatta ttcatgtggc aaacggaatc
    24241 acgcatattg gtccggcacg gcaacaggac ggcgattaca gttatctggc ggatattttg
    24301 caaaccgtgt tacctgacgt cgcgtggcat aacaatattc gcgccgagct gtggcgcaag
    24361 ctggcagtca actgtgtgat taatccactg accgccatct ggaattgccc gaatggtgaa
    24421 ttacgtcatc atccgcaaga aattatgcag atatgcgaag aagtcgcggc agtaatcgaa
    24481 cgcgaagggc atcatacttc agcagaagat ttgcgtgatt acgtgatgca ggtgattgat
    24541 gccacagcgg aaaatatctc gtcgatgttg caggatatcc gcgcgctgcg ccacaccgaa
    24601 atcgactata tcaatggttt tctcttacgc cgcgcccgcg cgcatgggat tgccgtaccg
    24661 gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga gtgaatatga gcgcatcggc
    24721 actggtttgc ctcgcccctg gtagtgaaga gactgaagcc gtcaccacta tcgatctgct
    24781 ggttcgcggc ggtatcaaag tcaccactgc cagcgtcgcc agcgatggta acctggcgat
    24841 tacctgctcg cgcggcgtga agctgctggc ggatgcgccg ctggtcgaag tagctgatgg
    24901 cgaatatgac gtgatcgtgc tgcctggagg cattaaaggc gcagagtgtt ttcgcgatag
    24961 caccctgctg gttgaaacca ttaaacagtt ccaccgttcc gggcgtatcg tcgcggctat
    25021 ttgcgccgca ccagccaccg tgctggtccc gcacgatatc ttcccgattg gcaatatgac
    25081 cggcttcccg acgctgaaag acaaaatacc cgccgaacaa tggcaggaca agcgcgtcgt
    25141 ctgggatgca cgggtaaaat tgctgaccag tcaggggccg ggtacagcta tcgactttgg
    25201 tctgaaaatt atcgacctgc tggttgggcg tgaaaaagcc catgaagtgg catcacaact
    25261 ggtgatggcg gcagggattt ataattatta cgagtagtgt cggatgcggc aaacgtcgca
    25321 tctgaccaga tgcgacgtaa aaaacacaaa ttacgggcga tataccttca cattgttaaa
    25381 gccctgctcg cgcagataga gcgcctgcag gcggctcatc accccgcgct cacaccacag
    25441 cagccaggtt ttgttctggt cgagatcgcc aaatttggtg ctcagtttat agaacggcag
    25501 agaaaccaca tcaatccctt cgactttcag cggcttatct tcctgttcat cgatagaacg
    25561 gatatcgagg atcacgtcgt tcgggccgaa gccattgacg gtttccactt ccaccacttc
    25621 ctgctcggtc tgctgggcga tttcgcggat atcaacgtta ttcgcttcct caaccacttt
    25681 atcgagaatg ctgaagtcga acttctcttc ttccgcttca atcttcgatt taaccgcttt
    25741 caccgtcggg cttttggaga tcacaccgca atattcgggc atcgtgcggg caaagtcttc
    25801 agtgccaatc tggcgagcca ggttgatgat gtgctctttg tcgtaagaaa tcagcggacg
    25861 caggatcagc gtgtcggaga cgttatcaat caggcgcagg ttagtcagcg tctggctgga
    25921 cacctggccc agtgcttcgc cggtgaccag cgcctgtacg ccgtaacgtt cagccacttt
    25981 agacgcggca cgcaccatca tacgtttgag gataacgccc atctgaccgt cgtcgatttt
    26041 ctcgagaatt tccccgacga ccggctcgaa attaatagcg acaaaacgca cgcggtggga
    26101 gctgccaaag cggttccaca gataatgtgc cacctgacgc acgccaattt catgcgccgc
    26161 gccgccgagg ttaaagaagc agtaatgcac gcggcagccg cgacgcatca acatataact
    26221 ggaaacaccg gagtcgaaac caccggaaat gagcgacagc acatcttcct gggtgccgat
    26281 cgggaaaccg ccaatacctt cgtagcgacc tttaatcagc aggagacgat cgtcttccac
    26341 ttccagatgg acagtcacat ccggattggt cagcttcacg cgcgcggatt caatatgctg
    26401 atttaaaccg ccgccgacgt aacgttccac atcaatcgag ctaaaatcat gtttgccacg
    26461 gcgcttcacg cgtacgcaga aggttttccc ttccagctga tcgcgatact gaaccaacgc
    26521 tttctcgaaa atatcgtgca tgtcggtaaa cggcacgtct tcgacttcga gaatatggtg
    26581 gatacccgga atacgggtca gggcgtcgcg aatagccaga cgctggtttt catcttttgc
    26641 gcgaacctcg atgttatccc agtggcggac gacagcgagc gtctcatcat agtgctttaa
    26701 aacgttacga atgttcccgg taaggatttt tataaagcgc aagcgcacag attggctttt
    26761 gatggtgatt tccgggaaca atttaatgat aaacttcatg gcggcaatgg ttcgttggca
    26821 agccttaagc gacttgtata gggaaaaata cagcagccca cacctgcggc tgcatccagg
    26881 cgcggaagta taccactaac atcgctttgc tgcgcacatc accttaccat tgcgcgttat
    26941 ttgctatttg ccctgagtcc gttaccatga cggggcgaaa aatattgaga gtcagacatt
    27001 cattatgccg aagaaaaatg aggcgcccgc cagctttgaa aaggcgctga gcgagctgga
    27061 acagattgta acccgtctgg aaagtggtga cctgccgctg gaagaggcgc tgaacgagtt
    27121 cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa ttacaacaag ccgaacagcg
    27181 cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta acccctttta caccggacaa
    27241 tgagtaatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa ccaggcgctg
    27301 agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac catgcagtat
    27361 ggcgcattat taggtggtaa gcgcctgcga cctttcctgg tttatgccac cggtcatatg
    27421 ttcggcgtta gcacaaacac gctggacgcg cccgctgccg ccgtagagtg tatccacgct
    27481 tactcattaa ttcatgatga tttaccggca atggatgatg acgatctgcg tcgcggtttg
    27541 ccaacctgcc atgtgaagtt tggcgaagca aacgcgattc tcgctggtga cgctttacaa
    27601 acgctggcgt tctcgatttt aagcgatgcc gatatgccgg aagtgtcgga tcgcgacaga
    27661 atttcgatga tttcagaact ggcgagcgcc agcggtattg ccggaatgtg cggtggtcag
    27721 gcattagatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga gcgtattcat
    27781 cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt aagcgccgga
    27841 gataaaggac gtcgtgctct gccagtactc gacaagtacg cagagagcat cggccttgcc
    27901 ttccaggttc aggatgacat cctggatgtg gtaggagata ctgcaacgtt gggaaaacgc
    27961 cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg tcttgagcaa
    28021 gcccggaaga aagcccggga tctgatcgac gatgcccgtc agtcgctgaa acaactggct
    28081 gagcagtcac tcgatacctc ggcactggaa gcgctagcgg actacatcat ccagcgtaat
    28141 aaataaacaa taagtattaa taggcccctg atgagttttg atattgccaa atacccgacc
    28201 ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag tttaccgaaa
    28261 ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag cgggcacttc
    28321 gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta caacaccccg
    28381 tttgaccaat tgatttggga tgtggggcat caggcttatc cgcataaaat tttgaccgga
    28441 cgccgcgaca agatcggcac catccgtcag aaaggcggtc tgcacccgtt cccgtggcgc
    28501 ggcgaaagcg aatatgacgt attaagcgtc gggcattcat caacctccat cagtgccgga
    28561 attggtattg cggtcgctgc cgaaaaagaa ggcaaaaatc gccgcaccgt ttgtgtgatt
    28621 ggcgacggtg cgattaccgc tggcatggcg tttgaagcga tgaatcacgc gggcgatatc
    28681 cgtcctgata tgctggtggt tctcaacgac aatgaaatgt cgatttccga aaatgttggc
    28741 gcgctcaaca accatctggc gcagctgctt tccggtaagc tttactcttc actgcgcgaa
    28801 ggcgggaaaa aagttttctc tggcgtgccc cccattaaag agctgctcaa acgtaccgaa
    28861 gaacatatta aaggcatggt ggtgcctggc acgttgtttg aagagctggg ctttaactac
    28921 atcggcccgg ttgacggtca cgatgtgctg gggcttatca ccacgcttaa gaacatgcgc
    28981 gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg ttatgaaccg
    29041 gcagaaaaag acccaatcac cttccacgcc gtgcctaaat ttgatccctc cagcggttgt
    29101 ttgccgaaaa gtagcggcgg tttaccaagc tattcaaaaa tctttggcga ctggttgtgc
    29161 gaaacggcag cgaaagataa caagctgatg gcgattactc cggcgatgcg tgaaggttcc
    29221 ggcatggtcg agttttcacg taaattcccg gatcgctact tcgatgtggc aattgccgag
    29281 caacacgcag tgacctttgc tgcgggtctg gcgattggtg ggtacaaacc cattgtcgcg
    29341 atctactcca ccttcctgca acgcgcctat gatcaggtgc tgcatgacgt ggcgattcaa
    29401 aaactcccgg tcctgttcgc catcgaccgc gcgggcattg ttggtgctga tggtcaaacc
    29461 caccagggcg cttttgatct ctcttacctg cgctgtattc cggaaatggt cattatgacc
    29521 ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta taacgatggc
    29581 ccgtcagcgg tacgctaccc gcgtggcaac gcggttggcg tggaactgac gccgctggaa
    29641 aaactgccaa ttggcaaagg cattgtgaag cgtcgtggcg agaaactggc gatccttaac
    29701 tttggtacgc tgatgccaga agcggcgaaa gtcgctgaat cgctgaacgc tacgctggtc
    29761 gatatgcgtt ttgtgaaacc gcttgatgaa gcgttaattc tggaaatggc cgccagccat
    29821 gaagcgctgg tcaccgtaga agaaaacgcc attatgggcg gcgcaggcag cggagtgaac
    29881 gaagtgctga tggcccatcg taaaccagta cccgtgctga acattggcct gcctgacttc
    29941 tttattccac aaggaacaca ggaagaaatg cgcgccgaac tcggcctcga tgccgccggt
    30001 atggaagcca aaatcaaggc ctggctggca taatccctac tctactcctg ctatgcttaa
    30061 gaaattattc atggcaggag tggcagcatg caatacaacc ccttaggaaa aaccgacctt
    30121 cgcgtttccc gactttgcct cggctgtatg acctttggcg agccagatcg tggtaatcac
    30181 gcatggacac tgccggaaga aagcagccgt cccatcatta aacgcgcgct ggaaggcggc
    30241 ataaatttct ttgataccgc caatagctat tccgatggca gcagcgaagg gatcgttggt
    30301 cgcgcactgc gggatttcgc ccgtcgtgaa gacgtggtcg ttgccaccaa agtgttccat
    30361 cgcgttggtg atttgccgga aggattatcc cgtgcgcaaa tattgcgctc tatcgacgac
    30421 agcctgcgcc gtctcggcat ggattatgtc gatatcctgc aaattcatcg ctgggattac
    30481 aacacgccga tcgaagagac gctggaagcc ctcaacgacg tggtaaaagc cgggaaagcg
    30541 cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg ctcaggcact ggaactacaa
    30601 aaacagcacg gctgggcgca gtttgtcagt atgcaggatc actacaatct gatttatcga
    30661 gaagaagagc gcgagatgct gccactgtgt taccaggagg gcgtagcggt gattccatgg
    30721 agcccgctgg cgcggggtcg tctgacacgt ccgtggggag aaactaccgc acgactggtg
    30781 tctgatgagg tggggaaaaa tctctataaa gaaagcgatg aaaatgacgc gcagatcgca
    30841 gagcggttaa cgggcgtcag tgaagaactg ggtgcaacac gagcacaagt tgcgctggcc
    30901 tggttgttga gtaaaccggg cattgccgca ccgattatcg gaacttcgcg ggaagaacag
    30961 cttgatgagc tgctgaacgc ggtggatatc accttgaagc cggaacagat tgctgaactg
    31021 gaaacgccgt ataaaccgca tccggtagta ggatttaagt aaatttcgct tttcagccat
    31081 tttatctgct gaattcccca tcgtctgcga tgatggggac aacgtacctc ccatcatctt
    31141 ctgtaaccat aatccacctc tgcaagagaa attcttcgtg aatgagtttg cagctattca
    31201 tcatcttcat ccctttgagc gatttttgat agtttctcca ggtaatactc cctaaaactg
    31261 tcttatttag tcaacgtaag aggattacaa tcatgatgaa aactatcacc aaacaaccga
    31321 tcctgtttac tgatgtaccg gttgcagatt taagaaactc aatgaagcac gatttaaatc
    31381 aaaatcttat cgaaaggcta tggaacaaaa ttcgtgattt ttttctgggc agtgataaac
    31441 aaaaagcatt taaatcgatt cataaataca tcaatacatt gtcagttcta aattataatt
    31501 cagcgtcatc tccagatccc aattttaata tagatgcatc gtcagattta gactcatatt
    31561 taaacctgaa atttgataac ctctcgccca aacaaaagca gaccacgctt tgttgtttct
    31621 ggaataaaat tgcatcctca ttgccagagc catacaacag caccatcaaa cataacatta
    31681 ttttttataa agtcggagaa aatctgatga tacgtggaac tatctctatt gttaacgagg
    31741 tagtaaagac ttattcactc ccaatagaaa aggatgacaa tggatattac gatttcagcg
    31801 gtctatatct agcacacagc aatatttccg gtaaagatcc caataaagat cccgatatag
    31861 atttcggtat agatctggga aactgtaact gctcaaatgt caacttcgaa catacttatt
    31921 ttgatagcgt caagtttaca aatacgaatt gtacaaatgc gaattttaac agttgtagat
    31981 ttatcaaatg cgatctaaca aatatgaatt gcactggcgc aattcttgat aatgcggtga
    32041 tatatggtaa agaaaaagaa cctgagatgc aatatccaga agcagatcaa ataatacaaa
    32101 ggataactta tcagaaatca catgggaatg aaacgaaagg gatgattcta actaactgct
    32161 cctgtgttaa aaccactttc aattgggcag atttatcaga aagtgattgc caaaatgtgg
    32221 actttagcga ggctaatctt tcaaacacta ttcttcctga catcgtcagg atgaaaggca
    32281 cgaaacttta ccgtaccgac ctttttaatc cgatattaaa agcagaagca gaatcaacag
    32341 aagaaaaaga tattagccca ttagcaaaaa ttattcttga ctatattgaa tcagataaaa
    32401 accctgagtc attaaaattt gatgaaaaat caacaaccac taaaataata caagatattg
    32461 ataattttat tttctataat cagcatttaa agagcatatt caatcaagca atgaatcttg
    32521 aaggaaaaat aagcagaaaa aaatacaatg agatttttaa ctataaacaa gaacaagcca
    32581 gacaat
//
LOCUS       DAOTRK010000045        31664 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.045, whole genome shotgun sequence.
ACCESSION   DAOTRK010000045 DAOTRK010000000
VERSION     DAOTRK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.045"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..520)
                     /locus_tag="QIE41_001991"
     CDS             complement(<1..520)
                     /locus_tag="QIE41_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530883.1"
                     /translation="MKQYEDGYNVNGYSWENETIEYLLNDKQLTDNHIPEWVKKNEIA
                     AFWFWLVIINTDPKLLGVRNELGFNTAMMGLIPAGSIPITTIPSDHEMRLDVISKWIN
                     ALILDNQSDWFISTMHQNWLQIEKRNEAKWLKKNTEQLEWAWKYINARIPHTYLSWFY
                     PVSDNERYIAIVV"
     gene            complement(1853..2383)
                     /locus_tag="QIE41_001992"
     CDS             complement(1853..2383)
                     /locus_tag="QIE41_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDS3530884.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAVMAITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            3053..3784
                     /locus_tag="QIE41_001993"
     CDS             3053..3784
                     /locus_tag="QIE41_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDS3530885.1"
                     /translation="MNQPIHNDYWLSRFESILNSALVQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRWANQRVHATSLRYFWCREFGKMYGRKHY
                     HVILLLNKDTWCSLGDFSEPSSLATMIQEAWCSALHLEPWQGDGLVHFSRWTPSRKPT
                     SSDARPSSDDTPLSGGCSDTRKASDKKPGEAAVLWIKRGDVEALQKARNRASYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
     gene            4305..4757
                     /locus_tag="QIE41_001994"
     CDS             4305..4757
                     /locus_tag="QIE41_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS3530886.1"
                     /translation="MHVINSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGKRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTEICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            5293..6228
                     /locus_tag="QIE41_001995"
     CDS             5293..6228
                     /locus_tag="QIE41_001995"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDS3530887.1"
                     /translation="MCDKEYIITIGSANIDIAGYSYASLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNSWLMSAVGDDFYGQSLLAQTTQSGVNVDKCLIVNGENTSSYLSLLD
                     KTGEMLVAINDMTISEHISAEFLSQHIDFIQGAKVIVADCNISESTLVWLLNNAGKVP
                     VFVDPVSAWKCVKIREHLAQIHTLKPNRLEAETLSGISLSDRNDAEKVAVWFHTHGLN
                     RLVLSMGGDGVYYSELYGASGWSPPVKTQVVNVTGAGDAMMAGLASCWVDNMPFIDSI
                     RFAQGCSSMALASEYTNNPELSVANVKSLVEKQNV"
     gene            6221..7156
                     /locus_tag="QIE41_001996"
     CDS             6221..7156
                     /locus_tag="QIE41_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDS3530888.1"
                     /translation="MSEITISSELLQISPEVQDAINSKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKHGAVPATIAVIGGVMKVGLSKDEIELLGREGHNVTKVSRRDLPFV
                     IAEGKHGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTRSLPAFFCRTSPFEVSIRLDSAKEIARA
                     MAVKWQTGLNGGLVVANPIPEAYAMAEEKINAAIDQAVKEAEVQGITGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAVLACEIAKEYQQIV"
     gene            7240..8490
                     /locus_tag="QIE41_001997"
     CDS             7240..8490
                     /locus_tag="QIE41_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDS3530889.1"
                     /translation="MDIMRSIVGMAVLLIIAFLLSVNKKRISLRTTGAALVLQIAIGG
                     IMLYFPPGKWLVEQAALGVHKIMSYSDAGSAFIFGSLVGDKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIRVMGGLIRILGGIFQKALNISKVESFVAVTTIFLGQNEIPAI
                     VKPFINRLNRNELFTVICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEESKVTFENLSFTDTPPKSVIEAVASGAMTGLKIAVGVATVVMTFVAIIALI
                     NGIIGGIGGWFGYGNITLEGIFGWILAPLAWIMGVDWADASLAGSLIGQKLAINEFVA
                     YLNLSPYLQPGSTLDVKTTAIISFALCGFANFGSIGVVVGAFSAISPQRAPEIAQLGM
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8509..9603)
                     /locus_tag="QIE41_001998"
     CDS             complement(8509..9603)
                     /locus_tag="QIE41_001998"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDS3530890.1"
                     /translation="MDNREKEILRILRHNPLIQQNEIAEILQISRSRVAAHIMDLMRK
                     GAIKGKGYILTEQEYCVVLGAINMDIRGVADIHYPQKSSSPGSIYCSAGGVGRNIAHN
                     LALLGRNVHLISAVGNDFYGETLLEQTRLAGVNVTNCIRLNGQNTSTYVSIADLQAET
                     VMAINDTHILQQLTPQLLDASRMLIRHAGVVLADCNLTPAALEWIFTVADGIPVFIDT
                     VSKFKAVNVKNWLPHIHTLKPTQKELEILWGSPVNNETDLLTAVNALHQQGVQQIFVW
                     MGDESVFCSQREGEQFLLTPPVHAVVDSFGADDGFMAGLLYSFLEGSSFQESAHFAMA
                     CAALSRASTSINNPTLSVNNAQYLLKTSRG"
     gene            10940..11506
                     /locus_tag="QIE41_001999"
     CDS             10940..11506
                     /locus_tag="QIE41_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDS3530891.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVLWIDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRNPHF"
     gene            11764..12336
                     /locus_tag="QIE41_002000"
     CDS             11764..12336
                     /locus_tag="QIE41_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530892.1"
                     /translation="MNAIPYFDYSLAPFWSSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEERLKELIWAF
                     SDFARDRRGVHDPEARCLAGNPWYPVAGQL"
     gene            12405..12641
                     /locus_tag="QIE41_002001"
     CDS             12405..12641
                     /locus_tag="QIE41_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDS3530893.1"
                     /translation="MLTSITGHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(12912..14243)
                     /gene="dsdA"
                     /locus_tag="QIE41_002002"
     CDS             complement(12912..14243)
                     /gene="dsdA"
                     /locus_tag="QIE41_002002"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDS3530894.1"
                     /translation="MENAKMNSLIAQYPLVEDLVALKEATWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPICGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAERLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYM
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGTD"
     gene            complement(14261..15598)
                     /gene="dsdX"
                     /locus_tag="QIE41_002003"
     CDS             complement(14261..15598)
                     /gene="dsdX"
                     /locus_tag="QIE41_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HDS3530895.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKILPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            15816..16760
                     /gene="dsdC"
                     /locus_tag="QIE41_002004"
     CDS             15816..16760
                     /gene="dsdC"
                     /locus_tag="QIE41_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HDS3530896.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHHFLMDEEILPVCSPEYAQRHDLTNTVINLSHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVCQSCQYQ"
     gene            16860..17186
                     /locus_tag="QIE41_002005"
                     /pseudo
     CDS             16860..17186
                     /locus_tag="QIE41_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            17477..18055
                     /locus_tag="QIE41_002006"
     CDS             17477..18055
                     /locus_tag="QIE41_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004133049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDS3530897.1"
                     /translation="MLLPMNTETQHRKKDPIRLYQQLLESAAMIAGRDGIAALSLNAV
                     AREAGVSKGGLLHHFPNKQALIYALFARLLAIMEEAIAVLMQKDNISYGRFTRAYVNY
                     LSALTDTQESRQLMVLSLAMPDEPVLRKCWRDWMLGHLANGDELDNSPTGTLVRYTAD
                     GIWLSELTEGITMSPEHRQALVDSLNKMTLPA"
     gene            18078..18896
                     /locus_tag="QIE41_002007"
     CDS             18078..18896
                     /locus_tag="QIE41_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDS3530898.1"
                     /translation="MRYNINARFIYDATDGTLTLPQSTEPDCQLSITSCALLNFFLHH
                     TDIVSREEVLTKVWDNNGLTSSNSNLNQYLSMLRKTFRHYGIDNIIITVARGYLQLNP
                     NVSIEPLDEAPAPPAIIEPSIIPDVDVPTSSEDTESSPPTLAPVTHRHDAYWYFAGAC
                     LLTISILLVAFNLFGISEARPIALTQLSHSQCELLASDEMRRSVSVKAYEKNYDEVRK
                     RLNIACKPSERFLFFYGDRLETNGLGRVFLAHCAMHENNPFSYCDNYFYYSWKL"
     gene            18896..19414
                     /locus_tag="QIE41_002008"
     CDS             18896..19414
                     /locus_tag="QIE41_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004108661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530899.1"
                     /translation="MRIAPRTFFAISALAFIVASGFSFWRLSPAENTGIMSCSTKGIM
                     RFENMEKENVNGNIHFNFGSQGKGSMVVEGYTDSAAGWLYLQRYVKFTYTSKRVSATE
                     RHYRISQWESSASSIDESPDVIFDYFMREMSDSHDGLFLNAQKLNDKAILLSSINSPL
                     WICTLKSGSKLD"
     gene            19934..20212
                     /locus_tag="QIE41_002009"
     CDS             19934..20212
                     /locus_tag="QIE41_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDS3530900.1"
                     /translation="MGDALGLIETKGLVACIAAADAMCKSANVELIGYENIGSGLVTV
                     MVKGDVGAVKASVDSGLESAQHIGEVVTSLVIARPHNDINKIVIKHKA"
     gene            20230..20514
                     /locus_tag="QIE41_002010"
     CDS             20230..20514
                     /locus_tag="QIE41_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDS3530901.1"
                     /translation="MGDALGLIETKGLVACIEAADAMCKAANVELIGYENVGSGLVTA
                     MVKGDVGAVKAAVDSGVESAQRIGEVVTSLVIARPHNDINKIVSHYKITD"
     gene            20529..20807
                     /locus_tag="QIE41_002011"
     CDS             20529..20807
                     /locus_tag="QIE41_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDS3530902.1"
                     /translation="MREALGLIETKGLVACIEAADAMCKAANVELIGYENVGSGLVTA
                     MVKGDVGAVNAAVDSGVEAARRIGEVVTSRVIARPHNDIEKIASQHKA"
     gene            20861..22447
                     /locus_tag="QIE41_002012"
     CDS             20861..22447
                     /locus_tag="QIE41_002012"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDS3530903.1"
                     /translation="MIELDTDLRSRQNARVLVRNAKKAQAIMATFSQQQIDAIVKNVA
                     QEAAHHAEALAKMAAEETGFGNWQDKVLKNRFASLRVYDAIKDMKTVGIIHDDQQQKV
                     MDVGVPLGVICALVPSTNPTSTIFYKTLIALKAGNAIIFSPHPGARQCSWKAIEVVKR
                     AAEAAGAPAGIVDGVTELTLEATQELMHSKDVSLILATGGEGMVRAAYASGTPTISGG
                     PGNGPAFIERSADIHHAVKDIITSKTFDNGVICASEQSIIVERCIYNEVHRELEAQGA
                     YFMNEDEAAKMAALLLRPNGTINPKAVGKTALYLSQMAGFCVPASTRVLIAEQTTVSH
                     KNPYSREKLCPVLGLYIEEDWKAACHRVVELLTNEGLGHTLVIHTRNQDVIRQFCLEK
                     PVNRILINTPAALGGIGATTNITPALTLGCGAVGGGSSSDNVGPMNLLNIRKVGYGVR
                     SIDELRSPGSRAEPQPAIAAPIVDPHRSILDDARFTSPAPATTSADDRFTVATTDPEG
                     EINEQNVERVIRQVLERLGK"
     gene            22474..22731
                     /locus_tag="QIE41_002013"
     CDS             22474..22731
                     /locus_tag="QIE41_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDS3530904.1"
                     /translation="MILAKVTGHVVATQKCDELRGSNLLLITQLDDNQQLMKDRSWVA
                     VDSVGAGVHDVVLAEEYFALNKDRYKAMSVVAIVEKVFRDA"
     gene            22747..23901
                     /locus_tag="QIE41_002014"
     CDS             22747..23901
                     /locus_tag="QIE41_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDS3530905.1"
                     /translation="MSEFLLKPRICFGQDALSVLDELTACHVLLVTDQAMVKFGLAER
                     VTQRLCARGIDWQVWDDVVADPDIATVVRGMKLMDSCYPDLVIALGGGSVIDAAKAVI
                     FALAQTRPEAGRERPCFVAIPTTSGTGSEVTSFSVVKAHAEKLVLVDSSLLPDIAILD
                     PSLVASVPPAITADTGMDVLCHALEAYVSLAASDFSDALAEKVVQQVFSYLPGCWRNG
                     QDLQAREKMHNASCMAGMAFTNASLGITHSLAHALGGVFRVPHGRANALLMAHVVAWN
                     ADYHGQCDTLAARKYARLAHLLDLPASTPREGVTSLLVAIQTLKDEMNMPRGINDTGV
                     NAADFDQRLAEMVAQALRDSCTPTNPREPDARALTELYRQAWSGNIAQCH"
     gene            23928..27314
                     /gene="cutC"
                     /locus_tag="QIE41_002015"
     CDS             23928..27314
                     /gene="cutC"
                     /locus_tag="QIE41_002015"
                     /EC_number="4.3.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline trimethylamine-lyase"
                     /protein_id="HDS3530906.1"
                     /translation="MANYNLTPRVKVLAERLLAHPSTLCVEHAGILSGLDGDIAGIPA
                     AVKPARRFYELMRQLPLAVSPDELIVGNQTHRPHGAIFHDESTAHRPSVFQFLNLNSD
                     LDAPDYKLVIEKGVLAIKQQLEEKTRSLGSAVSRSGMDEVNACRAAIYACDALMQLAQ
                     NLATSAEKLAATETNAYRKAELSESAAILHHIPARPARSFKEACQAFYLFQLALQLDN
                     GSYAVNPEGADKALLAYYQHDIANGLLTEAQAYEIVECLWFKLAELSEVRAACTIDGY
                     PMFDALLHGASLENAVINPLSEMFLNAQRNLSALNLPIRLFHGAHKTVTTLCAACNET
                     PVLEGLTPRIQRLRNHYLTVRPSVSIYRALAFTEVVKANPGMPTILLRAKAFRHACET
                     APILIQDDELIVGHPCGKPRAGAFSPDIAWRWVRDELDTMSTRPQDPFEISEEDKKTI
                     REEIVPFWEGRSLDEICEAQYREAGVWSFSGETFVSDLSYHQVNGGGDTCPGYDVLLF
                     TKGMNGIKADAEAHLAELSMENPEDIDRIYYYKAAIDTCEGVINYAHRIAARARELAA
                     VEQNAQRRAELLTIAEVNQNVPANPPKTLQEALQSIWTVESLFEIEENQTGLSLGRVD
                     QYCYPMFEADIREGRLTHEGALELMQAFIIKCAELMWMSSELGAKYFAGYQPFINLTV
                     GGQKRSGGDACNDLTYLIMDAVRFVKVYQPSLACRIHNQSPQKYMEKIVDVVKAGMGF
                     PACHFDDSHIKMMLRKGFDFEDARDYCLMGCVEPQKSGRIYQWTSTGYTQWPIAIEFV
                     LNRGRMVLFDSYQGLDTGDLKDLRTFEDFDAAVKKQVAHIIRLSAIGTVISQRVHRDV
                     APKPLMSLLVEGCMEKGKDVSAGGAMVNHGPGLIFSGLATYVDSMAAIRKLVYEDKKY
                     TLEQIRDALLANFEGYEGLRRDCLNAPKYGNDDNYVDQYALDITEWTERECRKYKMLY
                     STLSHGTLSISNNTPIGELTNATPNGRLAWMPLSDGISPTQGADKHGPTAIIKSVSKM
                     NVETMNIGMVHNFKFLKGLLDTPEGRHGLITLLRTASILGNGQMQFSYVDNEVLKKAQ
                     QEPEKYRDLIVRVAGYSAYFVELCKEVQDEIISRTVIEKF"
     gene            27366..28313
                     /gene="cutD"
                     /locus_tag="QIE41_002016"
     CDS             27366..28313
                     /gene="cutD"
                     /locus_tag="QIE41_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020316596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline TMA-lyase-activating enzyme"
                     /protein_id="HDS3530907.1"
                     /translation="MSANKELSGRIFNIQKYSIYDGDGIRTLIFFKGCNLRCPWCANP
                     EGLSSQFQVMFSQDKCINCGDCVNVCPAGIHYRAEVNGEMKHFVNRNKDCIGCRKCEE
                     ICTQNALDIMGKDVTVSELMEIIMQDYDFYVSSGGGVTIGGGEMSLQTDFAVALFSEC
                     KKMMINTAVETQGTTPLANYQKLAPVTDTFLFDIKQIDSNHHKTLFGIGNEGVRRNLE
                     WLVDSGANVIVRMPLIRGYNDSWEAITGAIDYVQKLAKRGNIRRIDILPYHQLGRKKY
                     ERLEMPYPIVEDPSYSVEELDKLEAFFAQFDFDIRLVRH"
     gene            28325..28789
                     /locus_tag="QIE41_002017"
     CDS             28325..28789
                     /locus_tag="QIE41_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDS3530908.1"
                     /translation="MNSLGVIETRGLVAAIQAVDAACKAAGVTCIGYRKVGSGLVTVC
                     FDGEISAVYTAIERGIAVASATDHQAKSLVIARPERCVVEALSNLKGHPPRVESGQKP
                     HAELPTVVEMAVCEETAEVSTDTETAMPVPEKEVQAPIVEVKSHGHKKGKKA"
     gene            28786..29406
                     /locus_tag="QIE41_002018"
     CDS             28786..29406
                     /locus_tag="QIE41_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDS3530909.1"
                     /translation="MIDALLQEKITTRLIDTAPTIPVGVSNRHVHLSQQDVEALFGVG
                     YQLTPFKPLRQPGQFAAEECVTVVGPKGSLTHVRVLGPVRPTSQLEISRADCFTLGIK
                     APVRESGQLENAGDALLVGPKGHVELHSQVICAWRHIHMSPQDARLLNVSNGQKVSVR
                     SHGERQLTFDEVVVRVRNDFALELHIDTEEANAAGLKNGAQVTLIR"
     gene            29418..30083
                     /locus_tag="QIE41_002019"
     CDS             29418..30083
                     /locus_tag="QIE41_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530910.1"
                     /translation="MTEQQIDTIVNLILQRLQPAVLVMVTSADGYRDLIHQRLARCGE
                     RLHLALDETISDSERWQQIGDVIPAKTWQHKLPSTPYKALLLPFLSYPLAVDIVNGTL
                     QSPVAQRVHDALLAGIPVLALRYYCDPHSELNALRGTVHSDYAAHLSATLTGLSECGI
                     TLCSMNEMLEKLATDVSSQSPVSHHRRYLTVTDIVNNPALAKSPDAVLTDAAVDFLKA
                     QKK"
     gene            30116..30517
                     /locus_tag="QIE41_002020"
     CDS             30116..30517
                     /locus_tag="QIE41_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012542991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDS3530911.1"
                     /translation="MSSKTKCWLWMLLVILSETSATSTLKMFDNSEGMTKTLLLALIV
                     VLYCICYYSLSRAVKDIPVGLAYATWSGTGILMVSTLGILFYGQHPDTAAIIGMVIIA
                     SGIIIMNLFSKMGSEEAEETPVTNLDKKIAN"
     gene            30533..30862
                     /locus_tag="QIE41_002021"
     CDS             30533..30862
                     /locus_tag="QIE41_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011705235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDS3530912.1"
                     /translation="MFNIGFLWLALSIGSEITGTSMIKKTNGFRRLWPSVLVVCAYST
                     CYFALTRAMSTIPVGVAYSLWCGFGIVGVTIFSMILYKQKPDLPAIFSMLLIISGGII
                     MNVFSNM"
     gene            complement(31086..31559)
                     /locus_tag="QIE41_002022"
                     /pseudo
     CDS             complement(31086..31559)
                     /locus_tag="QIE41_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8143 a         7468 c         7701 g         8352 t
ORIGIN      
        1 aaactacgat agcaatgtat ctttcgttat cacttacagg ataaaaccag gaaagatacg
       61 tgtgcgggat gcgtgcattg atatatttcc atgcccattc gagttgttct gtattctttt
      121 taagccattt ggcttcgttt ctcttttcga tctgcagcca gttttgatgc attgttgaga
      181 tgaaccaatc tgattgattg tcaaggatta atgcattaat ccatttgctt ataacatcca
      241 gtctcatttc atgatctgaa ggaattgttg ttatgggtat actccccgca ggaatcagcc
      301 ccatcatggc tgtattgaac cctaattcat tacgaacccc aaggagtttg ggatctgtgt
      361 taattataac taaccagaac cagaatgctg caatttcatt ttttttaacc cattcaggta
      421 tgtgattatc tgtcagttgt ttatcattca acaaatactc aattgtttca ttttcccagc
      481 tatatccatt cacgttataa ccatcttcat attgtttcat ctgattacgc ctcaatcgca
      541 ttctcttctg gtgattttca ctatcacatg gaaatcgaat atgcaaggaa gttaacaaaa
      601 aaaacattgc atccggactt gtgttcaaaa agtaatcact tcaaggcttg atcactctgt
      661 gagatgctga agaactattc tctaactatt cacgatctat tcttgatcta ttcataataa
      721 attaaaacat gctatcaact actgctcata tcatccttgg gtgtgtcgtt gtactgttcg
      781 actgttcttg tggtcagatt aacaatctga agagtaaaag cgacagtaat ctgtctgtta
      841 tagtggcgat aaaaacactg aatgcgcatc ctgtatgtga attcctgtca tgtggagcag
      901 taagcaatga cagacatgca tgttatatgt gaagggagcc gggagatgac ataatcagta
      961 tggctgtcat tctggttgta aaacgcgatc actttccggg ggtaaaagat gatatggaag
     1021 ctgacaaacg gtttgcttcg gattgtcttt tctggggcct gaaatacggg gacagaaaaa
     1081 taattccaga gtaaatctgc tctgttttca gaacagaaaa ctctatgctg gctgtaaaag
     1141 tgaaaaatca acactcagtc tgcatggatg tctgaagata ttgagaatgt cgggaatggt
     1201 actatatacg tattatacag acatatcttc actaagtgtt atacacgcat tttcatagac
     1261 aatcatacaa gtattttcat taagcattgc tgtacacatt gctttgtctg aaacacatcc
     1321 agtttagtaa atttgttagt ccttttttta cgatcgctaa aatcagttaa cctcactaca
     1381 aaaccctctg tcctgtttaa gcaatcaaga tgatattcga ttttactgaa cctgtgttta
     1441 accttttctg agttttcgtg ttttagtcgt taacctgaga aaagtatctt tacgtaaagc
     1501 ccagtgaatt aactcaaatc cgattgaggt aacctgatgc gatttactgt gagtttgcga
     1561 taacactgtt aaatggctta tggatgattt tgtaacggcg agattgataa ggtgattaac
     1621 tggccctggt gttttttctc agcgtaaggt gattatcttc atcataaggc tcttttcaga
     1681 aaaagtgtga tgattggttg tggtgttttg attcagatga ggatctgcca atgattatga
     1741 tgtattgtaa gaaataacgc cttcagaatg ttggagcatg cacttacaca aactgttttt
     1801 ctaaaattaa gggaacagtt taaagagtat aaactgttca tcttcttcag gattaagcta
     1861 cattgttgtt atgactgaca ggattgtttt cttcccctga ctctgggctt aacagtccct
     1921 ggcgttgtag atattcactg actgacattc cagaactacg gatcctgtca gagattttac
     1981 gaaattgatc cttccattct gcaggtaatt tcttaatttg tctgacttta caggctacac
     2041 tgtacggggt tcgccctagt ttctctgcta gccagaaaag gtgtttacca atttcatcgt
     2101 ccggtacatc ctccagaaca tctcgcaata aactctcgtc tgcctcattc catgccacac
     2161 cagcatttgg gaaacgagga tcgatctctc tttccttctt ctccttctta ctttgctctt
     2221 ttcgggatga tacagatacg acagaacgtt cttttaccgc tgtaattgcc ataactgcat
     2281 tgagcagaga tctgcgctcc acatcgttca gcatttttcc ttcacagatc aaatcattca
     2341 ggatgtcaat gactagattc agactttctt ctgttagctt catatttcag accttgaagt
     2401 atgtagataa tcagcacaat tactaatgtg ataaatatca gaagataatt tacaggtaaa
     2461 ccggaaaata catctgaaga ataaaggcct cagcttaacg tttcagccag tttgtgagct
     2521 gattgaggta cggcgatgac attaacggga attactcccc tatagctctg agcttatttt
     2581 tcaccctggc aacatatggt ggctactgcg catggttttg gagtagatat cttactactc
     2641 gtagaattgt gcttactggt caggccagcg cacaggcatt ccgtgcaatc aatagaacac
     2701 tggtttttta gtcttccgtt acccatcagg atgttagtgc agattccggt gtattcgatc
     2761 agttgttcgg cgaatcagcg atcgatcacg atgcgatttc gtatgttagg gatgctggta
     2821 tgattactcg ctgaaaaata atgtgaaaag gcagtttttc tttagacatt tagctcattc
     2881 atgctgttgt tttacgtttt gctgtcgtgt gcaggattat cttttcgtta cgggacgatt
     2941 cattctgttt taatcaggag ccattggcgt tgctcaatgg cgggatgccg taaagtttta
     3001 ccgcagtgat taatgatgtg aagtcaatcc aaatcaacgg agatctctca tcatgaatca
     3061 accaatacac aatgattact ggttatcccg ttttgaaagt attctcaaca gtgccctggt
     3121 gcaacaccgt gccgtctcgt taatccgggt ggatttacgt ttccctgagt atatgcctgt
     3181 caccatcatg gatcccgatc cggattcagc ggtgatttcc cgttttttcg catccctgaa
     3241 agccaaaatt caggcttacc agcggaaaaa aagatgggcc aatcagcgtg tgcatgcaac
     3301 cagcctgcgt tatttctggt gccgggagtt cggcaagatg tatggcagga aacattatca
     3361 cgtgatatta ctgctcaaca aagatacctg gtgctcgctc ggggatttca gcgaaccgtc
     3421 ttcgctggcg acgatgatcc aggaggcatg gtgcagcgcc ctgcatcttg agccctggca
     3481 aggcgatggc ctggtccatt tttccaggtg gacgccttcc cgtaaaccga catcgtctga
     3541 tgctcgccct tcttccgatg acacgccttt gtcgggtgga tgttctgata ccaggaaggc
     3601 ttcagacaaa aagccaggtg aagccgctgt gctctggatc aagcgtggtg atgtggaagc
     3661 gctgcagaaa gcccggaaca gagccagtta tctcgtgaaa tatgagacga agcagcatga
     3721 cggttccggg caacgtaatt atggttgcag tcgtgggccg gggcgtctac tggatggcag
     3781 gtgaacccgg taaaacagca tccggtgcct gagcgtatac cacggtcagg acgttggctg
     3841 acgcccttag ctcgttttct gataatcgcc ctgaagtcat gaaacggtat ttttatctgc
     3901 ttccattctc atgttcatat ttccccttac actttcactg tctgtcattg gaagtaccga
     3961 tttattttgg tgctgctcac ttactgccat tcacggaaga ggatagtggt gaaccgtacg
     4021 atcctgactg ctggcgaggg aaggttcaga actggtatga ggtttagtgc tggaagatga
     4081 ctttgaatcc cggactgagc agtgaggggg cagtgggagg atgctctggt gtaacaggcc
     4141 tggattgcaa aatacaaaac cggttgtaca ctcgtaacgg gcaaaaggat acgggcaata
     4201 acacgtggta cccggtgaga caggatcttg tatcgcatta ccgggaatat tctgacaggt
     4261 gaaatgttct gataagaatc atcctgaggg aggatacaac ggatatgcac gttattaaca
     4321 gcggcattcg tgatgtgaaa tttggtgaaa atgtgaaagt cgtcatgcca gtaaacctct
     4381 atgaatgtga actgggggat gatgttttta tcggtccctt tgtggaaata cagaagggat
     4441 gcatcattgg caagcggagc cgaattcagt cccacagttt catctgtgag aacgtcattt
     4501 taggtgatgg ctgtttcatc ggtcatggcg taacttttgc taatgatctt tttaagtgtg
     4561 gtggtcccga tgcctctgca gaaagctgga tacgtatcgt attaggtaac actgtaacag
     4621 tgggtagcgg agcgactatt ttaacaacag aaatttgcag tggagcagtg attggggctg
     4681 gtagtgtggt tacaaagccc gtgctggtaa aaggcgtgta tgccggtaat ccagccagac
     4741 ttttgagaac gctttaatta ttatatttac aaatcgtaag ccagaattga ctccagacca
     4801 ttatgtgcct tattcaccat gagtgaaatg cgggatgagc ctgcaagtta agaggatatc
     4861 ggaaaccagc aaccagggca gtgagagcgc tgtcggggtc attgatatct ccgaagggaa
     4921 gccctggtat aaggtgtttg cataaaatta ttaaacaggc tcatttttag cgatcctcat
     4981 gaaattcatc gtaacatcct gaatatctta tggtgtttac tgtcatctcc ggtatttatt
     5041 ccgttcatgc aaataacctg atgaaacagg gcaacgccat aaaaccggat aaggacaaca
     5101 tggattaacg ataaaaattg tgatcctgtt ttcatttttc actttttgat tctggactgg
     5161 tatttacatt tctaaaaaca ggcataaagt gaacacatgt ttgattttga atatatgttc
     5221 aactctatct gcgtagtcga atagaaatac agatttatta tttttgtgtt tttaaatgtc
     5281 aggagatgtg ccatgtgtga taaggaatac atcatcacaa ttggctctgc caatatagat
     5341 atagccggat attcgtatgc ctccttaaat tatgctgatt ctaacccggg gaagattaaa
     5401 ttcacgcccg gaggtgtggg gcgtaatatt gcacataatc ttgctctgct gggtaaaaac
     5461 tcatggctga tgtcagctgt gggagatgat ttctacgggc aatcattatt agcacaaacg
     5521 actcagtccg gcgttaatgt tgataaatgt ctgattgtta atggagaaaa tacctcaagt
     5581 tatttatcac ttctcgataa aaccggtgaa atgttggtgg caataaatga catgacgatt
     5641 agtgaacata tttcagctga gtttttatca cagcatattg attttattca gggagcgaag
     5701 gttattgttg cagactgtaa tatcagtgag tcaacacttg tctggctact gaataatgca
     5761 ggtaaggttc cggtatttgt tgacccggta tctgcatgga agtgtgtcaa aattcgtgaa
     5821 catttagcac aaattcatac tctcaagccc aaccggcttg aagcagaaac actcagtggt
     5881 atttcgcttt cagatcggaa tgatgctgaa aaagtggcgg tatggttcca tacacatggt
     5941 ctgaatcgcc ttgtgctcag catggggggg gatggagttt attacagcga actgtacgga
     6001 gccagcggct ggtcgccgcc agtaaaaaca caggtggtga acgttacggg ggcaggggat
     6061 gccatgatgg cagggctggc ttcctgctgg gttgataata tgccatttat tgattccatt
     6121 cgatttgcac aggggtgttc atcaatggcg ctggccagtg agtataccaa taatcctgaa
     6181 ttatctgttg ctaacgttaa aagcttagtg gagaaacaga atgtctgaaa taaccatttc
     6241 ttctgaatta ctgcaaattt caccggaagt acaggatgcc attaacagta aaaaacctgt
     6301 tgttgctctc gagtcaacaa ttatctcaca tggtatgcct tttcctcaga atgctcagac
     6361 ggctattgaa gtcgaagaaa ctatacgtaa acacggagca gtaccagcca ccattgccgt
     6421 tattggcggt gtaatgaaag tcgggttaag taaagatgag attgaattac tgggtcgcga
     6481 aggacataac gttaccaaag tcagtcgccg ggatttacct ttcgttattg ctgaaggaaa
     6541 acatggtgca acaacggtag cttcaaccat gattattgct gctcttgccg gaattaaagt
     6601 ttttgccaca ggaggaattg gcggcgttca tcgcggtgca gaacacactt ttgatatttc
     6661 agccgaccta caggaactgg cacatactaa tgtcaccgtg gtttgtgcag gcgcgaaatc
     6721 cattctcgat ttagggttaa ccacggaata tttagaaaca tttggtgtac ctttaattgg
     6781 ctatcagaca agatcattgc cggcattttt ttgccgcacc agtccgtttg aggtcagtat
     6841 acgtctggac agtgcaaaag agattgcccg cgcgatggca gtgaaatggc aaaccggcct
     6901 gaacggtggt ctggttgttg ccaatccgat tccggaagct tacgccatgg cggaagagaa
     6961 aattaatgca gcaattgacc aggccgtaaa agaagcggaa gtgcagggca ttaccggtaa
     7021 agaaagcaca ccgttcctgc tggcacgggt ggcagaatta accggtggtg acagtctgaa
     7081 atcaaatatt cagctggtat tcaacaacgc agttctggcc tgtgagatag caaaagaata
     7141 tcaacaaatt gtctgatata aatgaacggg cagtgtgagg tcgcccgaaa ttacctgaac
     7201 cagattccgt gtctggttca ggtataaggg gagagcatta tggacatcat gagaagtata
     7261 gtgggaatgg cagtattgct gataattgca tttctactat cagtgaataa aaaacgtatc
     7321 agtttacgta ccacaggagc tgcactggtt cttcaaatcg cgattggtgg gatcatgctc
     7381 tatttccccc cggggaaatg gctggttgaa caggctgcac tgggcgtaca taaaattatg
     7441 tcttacagtg atgcgggcag tgcctttatt tttggctctc tggtagggga caaaatggac
     7501 gtactgttcg atggggcggg ttttatcttc gccttccgtg tacttcctgc catcattttt
     7561 gttacagcgc ttatcagtct gctgtactac atcagagtga tgggggggct tatccgtatt
     7621 ctggggggga tttttcagaa agctctgaac ataagtaaag ttgagtcatt tgtggcagta
     7681 acgacaattt tcctggggca aaacgagatc ccggcaattg taaaaccgtt tattaatcgt
     7741 ctgaatcgta atgaattatt tactgtcatc tgcagtggga tggcttcgat tgccggttca
     7801 atgatgatcg gctatgccgg gatgggggtt ccgattgact atttgctggc ggcgtcgttg
     7861 atggcgatcc cgggggggat cctttttgcc cgtattctga gtccggccac agaagaatca
     7921 aaagtcactt ttgaaaatct ttctttcact gatactcctc ccaaaagtgt tattgaagca
     7981 gtggccagcg gggcgatgac cgggttgaaa atcgctgtcg gagttgcaac ggtagtgatg
     8041 acctttgttg ctattattgc tctgattaac ggtataatcg gtggtatcgg aggctggttt
     8101 ggttatggta atattaccct tgagggcatt ttcggctgga ttttggctcc ccttgcgtgg
     8161 atcatgggcg ttgactgggc ggatgcttca ctggcaggaa gtctgattgg tcagaagctg
     8221 gcaattaacg aatttgttgc ttatctcaat ctttcaccct atttacagcc aggcagtacg
     8281 cttgatgtga aaaccaccgc aattatttct tttgcactgt gtggttttgc caactttggc
     8341 tccattggtg tggtggtggg tgccttttca gccatatcac ctcagcgtgc acctgaaatt
     8401 gcacaactgg gcatgcgtgc actggcggcg gccaccctgt caaacctgat gagtgcgact
     8461 atcgctggtt ttttcattgg gctggcataa aaaatacaat cccgggtgtt acccccggga
     8521 tgtttttaac agatattgcg cgttattaac ggaaagtgtt gggttattga tgctggtgct
     8581 ggcacgtgac agtgctgcac atgccatcgc aaagtgagca ctttcctgga aactacttcc
     8641 ttccagaaaa ctgtaaagca gacctgccat aaagccatca tctgcaccaa agctgtccac
     8701 aacggcatga acgggcggtg tgagcagaaa ttgttctccc tctctttggc tacagaaaac
     8761 ggattcatct cccatccaga caaaaatctg ttgcacaccc tgctgatgta gtgcgttcac
     8821 tgcggtgagt agatcggtct cattgttgac aggggaaccc cataatatct ccagttcttt
     8881 ttgtgtgggt ttcagggtat ggatatgcgg aagccagttt tttacattaa cagccttaaa
     8941 tttcgacact gtatcaataa aaacgggaat gccgtcagcg accgtaaaga tccattcaag
     9001 ggcggcaggt gtcagattac agtcagccag caccacgcca gcgtgacgga ttaacatacg
     9061 tgaggcgtcc agcaactgcg gcgtcagttg ttgcagaata tgggtatcat taatcgccat
     9121 cactgtttct gcctgtaggt cggcgatgga gacataagtt gaagtatttt gtccgttcag
     9181 tcgaatacaa ttggtgacat taacgccggc caggcgggtc tgctccagta atgtttcgcc
     9241 ataaaaatca ttgcctacag cagatatcag atgcacattg cggcccagaa gggcgagatt
     9301 atgggcgatg ttgcgtccga cacctccggc agagcagtaa atactgcccg gactggagga
     9361 tttctgtgga taatggatat ccgccacccc acggatatcc atgttaatcg ccccaagaac
     9421 aacacagtat tcctgttctg tcaggatgta gcctttccct ttaatcgcac ctttgcgcat
     9481 cagatccata atgtgtgcag caacacgcga ccgactgatt tgtaaaatct cagcaatttc
     9541 attctgctgg atcagtgggt tgtgacgcag gatcctgagt atctcttttt ctctgttgtc
     9601 catgattatg caactgcctg ttcggttttt cgtcagcgat catcaaagta aatagcttcg
     9661 tttttcccgg tgaacgtgaa gctaggggag attatacttc cggatgttca ttcagggcag
     9721 aagaataata catcagttaa ttttctgtta gtactttgta gtcgaatgtt tggggagtgt
     9781 gtctgaatga cagccagatg gaatgacttt ttgtagccag gtcatgacta cgactcatac
     9841 gctattctta attcttattt ctttccatta tacgcaagta tttttaattt aggtttatta
     9901 ctataattat attcactttg tctttattca ctctgttttt acttactctc tatctttact
     9961 cattccatta ctctgaccct cacccgatta tacttactcc cttccttgtc taatcttcat
    10021 tgccaccatt atcatggtat ccaccttctc caccttctcc accttctcca ccttctccac
    10081 cttctccacc ttctccacct tctccttcat tcaatcataa ctactgtaat tcatacgata
    10141 ctcagataag ttattttact acgttgttgc taatatctac ctgttatcag tcttcactga
    10201 aacagcaatt gattttagtc attatccata atgaactgta ttgacttaac gatgattgtt
    10261 gttgacctga agtgttttaa ctattctggt aaatatcaat acttctgtca tcactgatta
    10321 actggatgtt tactctttga taaggtatcc atgatttcgt catgtttaac agcgcaggat
    10381 aaacaatgaa attaacagag tgaattcctg attatatttg ttcgtttctg attatcttgt
    10441 atgttaaaca aaatgaaaat tatccatcca tggtatattt tattctggta ccgtgtaccc
    10501 gttaacgctc atgttttgaa gtgagttgtt tttaacagat taaatgagag tgtatgattt
    10561 tatcaggtta cagatatgtg ttggccttgt acacgttttt gcagactcac tgagagcaag
    10621 tttgtagttt atgatgtgat tggtcgcaat atgtttctta atctccaggt agtggggttt
    10681 tagctcgtat tttacagtat ttcgtgatgt tttattgagt ctgtattttc tttactcctc
    10741 gtttatctca tctctttagc taataccatc agataatcca tttctttctg cataatgctg
    10801 cgtatcgtta ataacccgtc gtatccattc tgctacagca tgcctgataa ataccatctg
    10861 taagttatta ccgttatgag cgaaagcatt aattcgttca cagagcttaa aacatcatta
    10921 actttcagga gtcatcaaca tgcctaaatc ttacacacca aactggtttt ttaccgcttt
    10981 acttgacaat cacatcaatc aaatgatggc acgctattcc tgcctgcggg ccttacggat
    11041 ggatttcttc tacaggaaag atacgcccga tttcttacaa cctgatcatc gctggcttga
    11101 attgcagttg cgtatgctgc tggagcaggt ggaacaattt gaaaatatcg ttggtttctt
    11161 ctgggtgatt gaatggacgg ctgatcatgg ttttcatgcg catgcggttt tgtggatcga
    11221 tcgtcagagg gttaaaaaaa tatatccctt tgcggagcgg attacggaat gctggcggtc
    11281 tattacacat aacagcggtt cggcacaccg ctgtacatat cagccgcatt atacatacaa
    11341 catcaacatt cctgtgcgcc acaacgatcc tgaaagcatc gataatattc gcggtgccct
    11401 gcattatctg gcaaaagaag agcaaaaaga cgggctgtgt gcctacggct gcaatgaagt
    11461 tcctgaacgt cctgctgcag ggcgtcctcg taaccctcac ttctgaagct taaatcctga
    11521 gcttcctgtc ctggaaacat tccgtcggta aaaccttacc accttgctta atgatgtgaa
    11581 ctgaagtcaa cggagatcat tcactcttaa ccacatccgg tgttctgtcc cttgtcggtg
    11641 acctgatctt cccgttctgc ttcggttctt cacttattcc atcaacccca tccgcaagct
    11701 ataacacgtc agctcattca cgggcaggac gcattgtggg ctgcgcataa cggagcatat
    11761 cttatgaatg ctatccctta tttcgactat agccttgccc ccttctggtc atcttatcag
    11821 agcaaagtca tcggcgttct tgagcgtgcg ctgcgtgagc agtccggctc ccggatacga
    11881 cggatcctgc ttcgtctgcc atgtgaatat gacaatacct tcaacagcag aacgacctgg
    11941 tttggtatgg attttatcga aaccgtcagt gcgctgatga atgcgaaacc cggacgcgac
    12001 ctttgctggc tcctgacccg tcatcctgaa aagccggaat accacgtggt gctgtgtgtc
    12061 agacaggagt atttcgacgg ccctgaactg gaccggctga ttctggatgc ctggagcaac
    12121 gtactgggct tcgcgtcacc gggtgaagcg gcaccgtacc agaaacagat aacccgggat
    12181 gtggtgctgg acagtcgttc accggactgc gaagagagac tcaaagagtt gatatgggcg
    12241 ttcagtgatt tcgcccgcga tcgtcgtggt gtgcacgatc cggaagcccg ttgcctcgct
    12301 ggtaatccct ggtatcccgt agcaggccag ctctgaaagc tcggttcagc atctttccct
    12361 gcattcgcct aaccttcata aatctcacag aggatattct gaccatgttg acctcaataa
    12421 caggccacga cagcgtattg ctgcgtgctg acgatcccct gatcgacatg aactacatca
    12481 ccagtttcac cggtatgacc gataaatggt tttacaagct gatcagtgaa ggccatttcc
    12541 cgaaacccat caagctgggc cgcagcagcc gctggtacaa aagcgaagtg gagcagtgga
    12601 tgcaacagcg aatcgaggaa tcccgggggg cagcagcatg aaacgtgttg taatgccagt
    12661 acgctggcaa tgtgcaaaat gccagcgctg gtgggcacct acgttaccaa tactggctta
    12721 atggctacat acggcggtca gtttacgctt acgtgttaga catatattca agcctatctc
    12781 taatctggag atacagctac tgtctggtgt ttcagggggc tacatcagac aaaagatatg
    12841 aagtatcttt gagccacatc attttcccac aaaaagagag tcgatgtggc ataaaaaatc
    12901 attagtgacc cttagtcagt gcccttagcc agatattgtt ccatttcttc ttccggcacc
    12961 attccacctc ccgtcgccca caccagatga gtggcattac gcagttgttc tgcgctgaaa
    13021 ccgtgcatct gttggtaact tactgatgcg cacacgcgct gaggcccggc catacccgcc
    13081 agtgccgaag gttcaagacg aataccttct tcctgcgcca gccagccaag catgtcatac
    13141 atggtttgat cgctaagggt atagaagcca tccagcagac gctccatcgc ccgcccgaca
    13201 aagcccgatg cgcgaccaac cgcaaggcca tcggcagcgg taaggttgtc gatgccaata
    13261 tcctgaacag aaatctgatc gtgtaatccg gtatggacgc ctaataacat acacggggag
    13321 tgtgttggtt cggcaaaaaa gcagtgaaca tgatcgccaa acgccagttt aagtccgaat
    13381 gcgacgccac caggaccacc gccaacacca cacggcatat agacaaacag agggttatca
    13441 gcatcgacga tacggccttg ctgggcaaat tgcgctttaa gacgctggcc agcgacggaa
    13501 tacccaagga acaacgtgcg ggaattctca tcatcaataa agaaacagtt cgggtcagac
    13561 tgcgctgctt tacgtccttc ctcgacagca acaccataat cttgctcata ttccacgacc
    13621 gtaacgccat ggctgcgcag tttcgctttt ttccacgccc gggcatcagc agacatatgc
    13681 actgtcacct taaagccaat gcgggcgctc attatgccga ttgataaccc cagatttccg
    13741 gttgagccca cagcaatgct gtattgacta aagaactgtt taaactccgg ggaaagcagt
    13801 ttgctgtagt catcttcaag cgtcagcaac cccgcttcca gagccagtct ttctgcatgt
    13861 gccaggactt cataaatccc gccgcgtgct tttatggagc cggaaatagg caaatggcta
    13921 tcttttttca gtaacagttg cccacagatc ggttgttgat attccttttc cagccgtttt
    13981 tgcatagccg gaatggcaac cagttctgat tcaataatcc ccccagtggc agcagtttca
    14041 ggaaatgctt ttgccagata gggtgcaaaa cgggataagc gcgcatgggc gtcctgaaca
    14101 tcctgttcgg tcaggccaac ataaggtaaa ccttctgcca atgaggtagt gccaggatta
    14161 aaccaggtgg cttctttaag agcaaccaga tcctctacca acggatactg ggcgatgagc
    14221 gaattcattt tagcgttttc catagtacgt ctctttgcgc ttagatgata aatgacagca
    14281 ggaatgtgcc cgccagagcg acgaccgaag cgataaatgt cgctgtcgta tagtatttaa
    14341 atgtttcatt gagcgtagcg ccgcaatatt gcttcaccag ccagaaaagc gagtccgtaa
    14401 cgatcgtgca gccaatcgca ccggaaccga tagcaatcgc aataatttcc gggctgatgt
    14461 cgggatacag cggcagcatg ggggcaacaa ttgccgttgc ccccatcatt gccaccgtag
    14521 cggagcccac agcagcatgc agaataagtg ccaccaacca ggccagaaga atcgggtgca
    14581 tatgcatatt agagagaata actgccagcg tatcagcgag actgctgctt tttaaaatgg
    14641 cgttgaatgc gcctccggcc ccgataatca gcaaaatatt agcaatagaa ccgaaaccat
    14701 tttccgtatg tgtgagcatc gtccccatgc tcatatgctg acgtataccc aacacataat
    14761 aggcgacaaa cacggcgata aacatggcag tgatagggtt gccaataaac tcaagcaagg
    14821 tatacaaacc actctcacgc gccatattca attcggcaat cgttttaacc aacatcagcg
    14881 caatgggtag cagtatggtg aataacgttg cgcctaatga cggtagtatt ttttcatcgc
    14941 gaactttgag atcggcaaac tctgtgggta caggtttaaa gggcagtcgt tgccccagaa
    15001 atttaaggaa aagtgggcca ccgatcagtg atgccatcag cccaaccagc aaaccgtaga
    15061 cgatcaccga accgatatct gcgcccagct tattggcaac atataaagca gccggatgtg
    15121 ggggaaccac gcagtgcact gccatcaatg cggtacacag cggaatggcc agctttaaca
    15181 atgaggtatt ggtttttttg gcaattgaaa aagccagagg aatcaatagc acgacgccca
    15241 cttcaacaaa cagcgtgatg ccgcaaatca ggccaaccag caccataatg acatcagccg
    15301 aaagccaccg gcagcgttga agtgtcagac caattctttc tgcggccccg gatacttcca
    15361 tcatttttcc cagtatcgtg ccaaggccga taaccgcagc gaggaacccc agcgttccgc
    15421 caattccact ttcaatagca tttaccatat ccagtggccc catacccatc atcgttccca
    15481 cgaagaagct ggccagcaac agcgccagaa acgggtggaa tttgaacttc acgatggtca
    15541 gtacaattaa cacgatgctg ataagcagcg tgctcacaac ccagatttga gagtgcatat
    15601 ctcaccttcc cctgtgatta acctgatgat attggacaaa aaaacaacgc aggctgacaa
    15661 acgataaaat ttgcctttca catgagctaa attgcatcaa gggagtgatt tacatctaaa
    15721 aatgaaggtg aattgagata tggttcactt tagctcacct tacagctgaa attttgccgc
    15781 tattttttta cacttaatag aaaaatgagg tgattatgga accccttcgt gaaataagaa
    15841 atcgactgct taacggctgg caactatcaa aactgcatac ttttgaagtg gctgccaggc
    15901 atcagtcttt cgccctggcg gcagaggaat tgtcgctgag ccccagtgcg gtaagtcacc
    15961 gtatcaatca gctggaagaa gaattaggta ttcagttgtt tgttcgttcc catcgcaaag
    16021 tggaattaac gcacgaggga aaacgcgttt attgggcgct aaaatcgtcg ctggataccc
    16081 tgaatcagga aattctggat atcaaaaatc aggagttatc gggaacgtta actctgtatt
    16141 cccggccctc tatcgcccaa tgctggttgg tgcccgcatt aggtgacttt acacgccgat
    16201 atccgtctat ttcgctcacc gtgctcactg gtaatgacaa tgtcaatctg caacgtgccg
    16261 gaatcgattt ggcaatctat tttgatgacg caccctcagc gcaactgact catcactttc
    16321 tgatggatga agaaatcctg cctgtatgta gcccggagta cgctcaaaga catgatttaa
    16381 ccaacacggt aattaacctt agccactgta cgttgctcca tgacagacag gcctggagca
    16441 acgactccgg tacggatgaa tggcatagtt gggcgcaaca ttatgcggtt aatttgccga
    16501 catcttctgg aattggcttt gatcgttctg atttagctgt tatcgccgcg atgaatcata
    16561 ttggggtggc gatgggaagg aaacgcctgg tacaaaaaag gcttgccagt ggtgagctcg
    16621 tcgcgccgtt tggcgatatg acggtgaaat gccatcagca ttattacatc accacattac
    16681 cgggcaggca gtggccaaaa attgaggcat ttattacctg gctaagagag caggtatgtc
    16741 agtcttgtca gtatcagtga taccggggat accgacaata tggaagggct cattgcccta
    16801 aaaacaacat tggtgtcgta tgtaccggag tcgcagacat tgcgctccgt catttgtacg
    16861 gcacagccca tcacagtgat aaaggttctc agtatgtatc agggatctac acgctgcggc
    16921 ttaaggaagc cggattactg gcatcaacag gaagtacagg tggcccgcat gacaacgcga
    16981 tggcggagag catcaatggt ctttacaaag cggaggtaat acaccgtaag agctggaaaa
    17041 accgtgcaga agtggaactg tccacactca cgtgggtgga ctggtataac aatcgacgat
    17101 tgctgggaag gctgaggcat actcctccgg cagaagcaga aaaagcgtat tatgcttcca
    17161 tcggaaatga tgagctggca gcctgagttc acagataaaa cactctctag gaaccccggg
    17221 gcggttcatt ctcgcttgcc ttctgtattt caaaaagcat cattcaggtc attaaaagtt
    17281 atttgatcat ctttttatgg atttgctgtt catcgaaagg gtaacagatt cccaccattg
    17341 tgcagactag gagaattaac agcagcagta ggatttatac gacaaattgt gaccttacac
    17401 gcaataccca tactcacttc atgggtagct gcttctcctt gatagcgatc aaaaaaaaac
    17461 ataccgtctg gtcggtatgc ttctgcctat gaatacagaa acccaacatc gtaaaaaaga
    17521 tcccatccgt ctgtatcagc agttgctgga gtctgcagct atgattgctg gtcgtgacgg
    17581 tatcgctgcc ctctcgctga atgcggttgc ccgcgaagct ggcgtgagta aaggtggatt
    17641 actgcatcac tttccgaaca agcaggcgct gatctacgcc ttgtttgccc ggttgttggc
    17701 aataatggaa gaggccatcg ctgtcctgat gcaaaaagac aatataagct atggccgttt
    17761 tactcgcgca tatgtgaact atctttctgc gctgactgac acacaagaaa gccgtcaatt
    17821 gatggtgctt tcgctggcaa tgcccgatga accggtgctg cgaaaatgct ggcgagactg
    17881 gatgcttggt catttagcga atggcgatga actggataat agcccaaccg gaacgctggt
    17941 gcgctatacc gcagatggca tctggttgtc tgagttaacg gaagggatca ccatgagccc
    18001 cgaacatcgt caggcactgg ttgattcact gaataagatg acacttcccg cgtgaagtcc
    18061 catggattga ggtcgcgatg cgctataaca tcaatgcccg tttcatttat gacgctacgg
    18121 atggcacgct tacgctaccg caaagcactg agccagactg ccaactctcg ataacgtcct
    18181 gcgcgctgct caactttttt ttgcatcata ccgatatcgt tagtcgcgaa gaggtactga
    18241 caaaagtttg ggataacaac ggattaactt cttctaacag caatctgaat cagtatctca
    18301 gtatgctgcg caagacgttc cgtcactatg gtattgataa tattatcatt accgttgcac
    18361 gcggctatct gcagctaaac ccgaatgtat ccatcgaacc actggatgaa gcccctgcgc
    18421 cacctgcaat tatagagcca tccataatcc ctgatgttga cgtaccaaca tccagtgagg
    18481 ataccgagtc ttcaccacca acattagcgc cagtcaccca tcgtcacgac gcttactggt
    18541 attttgcagg tgcctgtttg ttaaccattt cgattctgtt ggtagccttc aatttgttcg
    18601 gtatcagcga ggcgcgcccc atcgctttga cgcaactttc tcatagccag tgcgaattac
    18661 tggcaagcga cgaaatgcgc cgttcagtaa gcgtgaaggc atatgagaaa aattatgacg
    18721 aagtccgtaa gcgtctaaat atcgcctgta aaccaagtga gcgttttctt tttttctacg
    18781 gtgacaggct ggaaaccaac ggccttgggc gagtgttcct cgcgcactgc gctatgcatg
    18841 aaaataaccc gtttagttat tgcgataact acttttatta ctcttggaaa ctgtaatgag
    18901 aatcgcaccg cgtaccttct ttgctatttc cgccctggcg tttattgtcg cctccggatt
    18961 tagtttctgg cggttgtccc ctgctgaaaa tacagggatt atgagttgtt caacaaaagg
    19021 catcatgcgt tttgagaata tggaaaagga gaacgttaac ggtaatattc actttaactt
    19081 tggcagccag ggtaaaggtt cgatggtggt cgaaggctac acggactctg ccgctggctg
    19141 gctgtacctg caacgctatg tcaaatttac ctataccagt aaacgtgttt ccgccacgga
    19201 acgccattac cgcatcagcc agtgggaatc cagcgcctca tcgatagatg aatcaccaga
    19261 tgtgattttt gactacttta tgcgtgaaat gtctgacagc catgacgggc tgttcctcaa
    19321 cgcccagaag ctgaacgata aagcgatttt gctcagttct attaattcac cgctttggat
    19381 ctgtaccctt aaatctggca gcaaattaga ctagtttttt ctggctgcct tacgcaacga
    19441 attgctcacc actttcatcg tccccccttc gcctgacgag aaacacgact ctttaacttc
    19501 gtgatagcac aggcgatttg tgatgtacca cgcattccat tttccccttc tcaacggccc
    19561 tgagcaaaag cgcatttttt gcgtttaggg tttgcgccaa agtatgagcc ccaacacact
    19621 tcaccgctgt agctgttacc cccttttcgt aattatttag caacttagca acaaacatac
    19681 ccggcgaaaa aaatacgcca aaaagcgcat tattaaaaac aaaatcctcc ccaccactcc
    19741 agcacaaata cataaatcag agtgtattga aagctaaatt tcagaattta attctaaatc
    19801 attgctgtca gcagatttaa attccacaaa attccatgag ctctatagtg acattcgaac
    19861 gccgcttccg tgagttaacg ctgtgtcacg gacggttttc ctgaatcaca aaacctgttc
    19921 tgaggagatc accatgggtg atgcattagg gcttatcgaa accaaaggcc tggtggcctg
    19981 tattgccgca gcggatgcga tgtgtaaatc cgccaacgtt gaactgattg gctatgaaaa
    20041 catcggctct ggtctggtga ccgtgatggt gaaaggcgac gttggcgcag tgaaagcatc
    20101 tgtcgattct ggacttgagt ccgcacagca catcggggaa gtggtgacgt cgctggttat
    20161 cgcgcgtcct cacaacgaca tcaacaaaat cgttattaaa cataaagctt aaggccggag
    20221 ggaagaaaca tgggtgatgc attaggtctg attgaaacca aaggtttggt ggcttgtatt
    20281 gaagcagctg acgcgatgtg taaggccgcc aacgttgagt tgattggcta tgaaaacgtc
    20341 ggttctggtc tggtcaccgc aatggtgaaa ggtgatgtcg gcgcagtaaa agcggcggtg
    20401 gattccggtg ttgagtccgc acagcgcatt ggcgaggtgg tgacgtcgtt ggttatcgcg
    20461 cgtccacaca atgacatcaa caagattgtc tcgcactaca aaattaccga ttaaccggag
    20521 aaaacataat gagagaagcg cttggtctga tcgaaaccaa aggtctggtg gcctgtattg
    20581 aggcggcaga cgcgatgtgt aaagccgcca acgttgagtt gattggctat gaaaacgtcg
    20641 gctctggcct agtcaccgcg atggtaaaag gcgacgttgg cgcagtgaat gccgcggtgg
    20701 attccggtgt tgaagcagca agacgtatcg gtgaagtggt gacctcccgt gtcatcgccc
    20761 gcccgcataa cgacatcgaa aaaatcgcgt cgcagcataa agcatgacct gacagggcac
    20821 gccccgtctg cactctttta gtctgatgaa ggatagactc atgattgaac tggataccga
    20881 tttgcgctcc cgtcagaacg cgcgggtgct ggtgcgtaac gccaaaaaag cgcaagcgat
    20941 tatggccact ttttcgcagc agcaaatcga cgccatcgtg aaaaacgtgg cccaggaagc
    21001 agcacaccac gctgaagcac tggcaaaaat ggccgcagaa gaaactggct tcggcaactg
    21061 gcaggataaa gtcctgaaaa accgtttcgc ctcgctgcgt gtttacgatg ccatcaaaga
    21121 tatgaagacc gtcggcatca ttcatgacga tcaacagcaa aaagtgatgg acgtgggcgt
    21181 gccgctgggt gtgatttgcg ccctagtgcc gtcaaccaac ccgacttcca ccatcttcta
    21241 caaaacgctg atcgccctga aagccggtaa tgcgattatt ttctctccgc atcccggcgc
    21301 acgtcagtgt agctggaagg cgatagaagt cgttaagcgt gccgcagaag ctgctggtgc
    21361 acctgcgggt attgtcgatg gtgtaaccga gctcacgctg gaagcaaccc aggaactgat
    21421 gcatagcaaa gatgtttcgc tgattctggc taccggtggt gaaggaatgg tacgagcagc
    21481 atatgcttct ggcacaccga ccatcagcgg tggtcctggt aacggtccgg catttattga
    21541 acgcagtgcg gatatccacc atgcggtgaa agatatcatc accagcaaaa ccttcgataa
    21601 tggtgtgatc tgcgcttctg aacagtcgat tattgttgaa cgctgcattt acaacgaagt
    21661 acaccgcgaa ctggaagctc agggtgctta ctttatgaac gaagatgaag cggcaaaaat
    21721 ggcagctctg ctgctgcgcc cgaacggcac gattaacccg aaagcggtgg gaaaaaccgc
    21781 actgtattta agccagatgg ccggtttttg tgtgcccgcc agtaccagag tactgattgc
    21841 cgaacaaact accgtttctc acaaaaaccc ctactcacgt gaaaagctgt gcccggttct
    21901 tggtctgtat atcgaagagg actggaaagc tgcctgccac cgcgtggtgg agttgctgac
    21961 taacgaaggt ctgggccaca cactggtgat ccatacccgc aaccaggatg tgatccgtca
    22021 attctgcctt gaaaaaccgg ttaaccgcat tctgatcaat acccctgcgg cgcttggcgg
    22081 tattggcgct accactaaca ttacccccgc cctgacgctt ggatgcggtg cggtaggtgg
    22141 tggttcctct tccgataacg tcgggccaat gaatctgctc aacattcgta aagtgggcta
    22201 tggcgtgcgt tccatcgatg agttgcgttc tccgggtagc cgtgcagaac cgcagccagc
    22261 catcgccgcg ccgatagtgg atccgcatcg cagcattctt gacgatgccc gcttcacctc
    22321 ccctgcccca gcaaccacat ctgctgatga tcgttttact gtggcaacga ctgatcccga
    22381 aggcgagatc aacgagcaga acgttgagcg agtcatccgc caggtgcttg agcgcttagg
    22441 caagtaattc attgagataa ggcgataaaa acgatgattc tcgcaaaggt aaccggacat
    22501 gtcgtcgcca cgcaaaagtg cgatgagctg cgcggcagca acctgttgtt gatcacccaa
    22561 ctggatgata accaacagct gatgaaggac cgctcctggg tcgctgtcga tagcgtcggt
    22621 gcaggcgttc atgacgttgt gctggcggag gagtacttcg cactcaataa agatcgttac
    22681 aaagcgatgt cagtggtcgc cattgtcgag aaggtttttc gggacgctta aggagtgaaa
    22741 aaaccaatga gtgagttttt gctgaaaccc cggatctgct tcggtcaaga cgcgctgtca
    22801 gtcctggacg agctaaccgc ctgtcatgtt ttgctggtta ccgatcaggc gatggtcaaa
    22861 ttcggtcttg ctgaacgtgt cacgcagcgt ctctgtgcgc gcggcattga ctggcaggtg
    22921 tgggatgacg ttgtcgccga tccggatatc gctaccgtgg tccgaggaat gaagttgatg
    22981 gacagctgct acccggatct ggttatcgca ctcggcggtg gttcggtcat tgacgcagcc
    23041 aaagcggtta ttttcgccct ggcgcaaacc cgtccggaag caggacgtga acgcccctgc
    23101 tttgtggcta ttccaaccac cagcggtacg ggctcagaag tgaccagttt ctcggtggtg
    23161 aaagcccatg ccgaaaaact ggtgctggtt gactcgtcgc tgctaccgga tatcgccatt
    23221 cttgatccgt cactggtggc atccgtgcca cctgctatca ccgctgacac cggaatggat
    23281 gtgttgtgcc acgctctgga ggcttatgtc tcccttgccg ccagcgactt ttccgatgca
    23341 cttgctgaaa aagtggtgca acaggtgttc agctacctgc cgggctgctg gcgtaacggc
    23401 caggatttgc aggctcggga gaaaatgcac aacgcctcat gtatggcagg aatggcgttt
    23461 accaatgcct ctcttggcat cacccatagc ctggctcatg cccttggtgg tgttttccgt
    23521 gtccctcatg ggcgcgcaaa tgccctgcta atggcacacg ttgtggcatg gaatgctgac
    23581 taccacggtc aatgcgacac ccttgctgcg cggaaatatg cacgtctggc acatctgttg
    23641 gatctgcctg ccagcacgcc tcgtgaagga gtaacaagcc tgctggtcgc aatccaaacg
    23701 ctgaaagatg aaatgaatat gcccaggggg attaacgaca ccggcgtgaa tgccgctgat
    23761 ttcgatcagc gcctggcgga gatggttgcc caggcactgc gtgacagttg caccccaacc
    23821 aacccgcgcg aaccggatgc ccgcgccctg actgaacttt atcgccaggc ctggagcggc
    23881 aatatcgctc aatgccactg ataacaagaa ctggagaccc ttaccttatg gcaaactaca
    23941 atttaacacc gcgcgtaaaa gtgctggctg aacgtttact ggctcacccc agtaccctgt
    24001 gcgttgaaca tgccgggatc ctgagtgggc tggatggtga tatcgcgggc attcccgccg
    24061 cagtaaaacc cgctcgccgt ttctatgaac tgatgcgcca gctaccgctc gctgttagcc
    24121 ccgatgaact gattgttggc aatcagactc atcgtccgca tggtgcgatt ttccacgacg
    24181 aaagtacggc ccaccgcccg tctgtttttc agtttcttaa tctaaatagc gaccttgatg
    24241 ccccggatta taaactggtg atcgaaaaag gcgtgctggc aatcaaacag caattggaag
    24301 agaaaacccg ctcactgggc agcgccgtta gccgcagtgg tatggatgaa gtcaacgctt
    24361 gccgcgccgc gatttatgcc tgtgatgccc tgatgcagtt agcgcaaaat ctggccacca
    24421 gcgccgaaaa actggctgca actgagacaa atgcgtatcg caaagctgag ttatctgaaa
    24481 gtgccgccat tttgcaccat atcccagcgc gtcctgctcg tagttttaaa gaggcttgcc
    24541 aggcatttta tctgttccag ttagcactcc agttagataa tggtagctat gctgttaacc
    24601 cggaaggcgc agataaagcg ctgctggcgt attatcagca tgatattgcc aacggtctgc
    24661 tgaccgaggc gcaggcttac gaaattgtcg aatgtttgtg gtttaaactg gcagaactga
    24721 gtgaagtccg cgcagcttgt actatcgatg gctacccgat gttcgatgct ctgttgcacg
    24781 gtgcaagcct tgagaacgca gtgattaacc ctctttcaga gatgttcctc aatgcacaac
    24841 gtaacctgag cgcgctaaat ctgcccatac gtctgttcca tggcgcccac aaaaccgtta
    24901 ccacactatg tgccgcctgt aacgaaacgc cagtgctgga aggcctgaca ccacgtatcc
    24961 aacgtctgcg taaccactac ctgactgtcc gcccgagtgt ttctatttac cgtgcactgg
    25021 cttttaccga agtagtgaaa gcgaatccag gaatgccaac catcctgctg cgtgctaaag
    25081 cgttccgtca cgcctgtgaa actgcgccga ttttgattca ggacgatgag ctgatcgtcg
    25141 gtcatccttg cggtaaaccg cgtgctggcg cattttcacc tgatattgcc tggcgctggg
    25201 tgcgtgacga actcgatacc atgagtactc gcccgcagga tccgtttgaa atcagcgaag
    25261 aagataaaaa aaccattcgc gaagagattg taccgttctg ggaggggcgc tcgctggatg
    25321 aaatttgtga agcacaatac cgcgaagctg gcgtttggtc gttcagtggc gaaacattcg
    25381 tcagcgacct ctcctatcat caggttaacg gtgggggtga tacctgtccg ggctacgatg
    25441 tgctgctgtt taccaaaggg atgaatggca tcaaagccga tgccgaggcg catcttgccg
    25501 aactgagcat ggaaaacccg gaagatatcg atcgcattta ctactacaaa gcagcgatcg
    25561 acacctgtga aggggtgatc aactatgcgc atcgtattgc cgcccgtgcc cgtgaactgg
    25621 ctgccgttga acagaacgcc cagcgtcgag cagagctact gactatcgca gaagttaacc
    25681 agaacgttcc ggcgaatccg ccgaaaacct tgcaggaagc gctgcaaagc atctggacgg
    25741 tagagtcact gtttgagatt gaagagaacc agacaggcct gtcacttggg cgtgttgacc
    25801 agtactgcta cccgatgttt gaagcagata tccgtgaagg tcgcctgact catgaaggtg
    25861 cactggagct gatgcaggca tttatcatca aatgtgccga gctgatgtgg atgtccagcg
    25921 aactgggagc gaaatatttt gcgggttatc agccatttat caacctgact gtcggtggtc
    25981 aaaaacgtag cggtggcgat gcctgcaacg atctcaccta tctgattatg gatgcagtcc
    26041 gttttgtgaa agtgtatcag ccatcactgg cttgccgtat ccataaccag tcgccgcaaa
    26101 aatatatgga aaaaattgtc gatgtggtga aagcggggat gggcttccca gcctgtcact
    26161 tcgatgactc ccatatcaaa atgatgctgc gcaaaggctt tgactttgaa gatgcccgtg
    26221 attactgcct gatgggctgc gtggaaccac aaaaatcagg tcgtatttac cagtggactt
    26281 ccaccggcta cacccaatgg ccaattgcca tcgagtttgt cctcaaccgt ggtcgtatgg
    26341 tgctgtttga tagctatcag gggctggaca cgggcgatct gaaagatctg cgcacattcg
    26401 aagattttga tgccgcggtg aaaaaacaag ttgctcatat catacgcttg tccgccatcg
    26461 gtacggtcat cagccagcgt gtgcaccggg acgtggcacc aaaaccgctg atgtcgctac
    26521 tggttgaagg ctgcatggaa aaaggcaaag acgtctctgc cggtggcgca atggtaaacc
    26581 acggacctgg gctgattttc tctggtctgg ctacctacgt cgattcaatg gcggccatcc
    26641 gcaaactggt ttacgaagat aagaaatata cccttgaaca gatacgggac gctctgctgg
    26701 caaactttga aggttatgaa ggcttgcgtc gcgactgtct gaacgcaccg aaatacggca
    26761 acgacgataa ctacgttgac cagtacgctc tggatatcac cgagtggact gaacgtgaat
    26821 gccgcaagta caagatgctc tactccaccc tcagccacgg tacgttgtcg atctccaaca
    26881 atacgccgat tggtgagctg accaatgcca caccaaatgg tcgtctggcg tggatgccgc
    26941 tttctgacgg gatcagcccg actcaagggg cggataagca tggcccaacg gcgattatca
    27001 agtctgtgag caaaatgaac gttgaaacca tgaatatcgg catggtgcat aacttcaaat
    27061 tcctcaaagg gttacttgat acacctgaag gacgccacgg cctgataact ctactgcgta
    27121 cagcatcaat tctcggcaac ggtcagatgc agttcagcta cgtcgataac gaagtactga
    27181 aaaaagcaca acaggaaccg gaaaaatacc gtgatttgat cgtccgtgta gccggttaca
    27241 gcgcctactt cgtggaattg tgcaaggaag ttcaggacga aatcatcagc cgtacggtga
    27301 ttgagaagtt ctgataaaac ggatcgcaac cggacaagga tgtccgacat tttcaggagg
    27361 caagcgtgag cgcaaataaa gaattaagcg gacgaatttt caatatccag aaatattcga
    27421 tctacgacgg tgacggtatc cgcacactga ttttttttaa aggctgcaat cttcgctgcc
    27481 cgtggtgtgc caacccggaa gggttaagca gccagtttca ggtgatgttc tctcaggata
    27541 aatgcatcaa ctgcggcgac tgcgtcaatg tatgccctgc ggggatccac taccgcgcgg
    27601 aagtcaacgg agaaatgaag catttcgtca accgtaataa agactgcatt ggttgtcgca
    27661 agtgtgaaga aatttgcacc caaaatgcac tggatatcat gggtaaagat gtcacggtca
    27721 gtgaattgat ggagatcatc atgcaggact atgactttta cgtctcctcc ggcggtggtg
    27781 tcactattgg gggcggtgag atgagcttgc aaactgattt tgccgttgcc ctgttcagtg
    27841 aatgcaaaaa gatgatgatc aacaccgccg tcgagaccca gggtacaacc ccgcttgcaa
    27901 actatcagaa gctggctccg gtcaccgaca ccttcctgtt cgatatcaaa cagatcgata
    27961 gcaatcacca taaaacgctg ttcggaattg gcaacgaagg cgtgcgtcgc aatctggagt
    28021 ggctggtgga ttcgggggcc aacgtgattg ttcgtatgcc gctgattcgt ggttataacg
    28081 attcgtggga agcgattacc ggcgctatcg actacgtcca gaaactggcc aaacgcggca
    28141 atattcgccg aatcgatata ctgccgtacc accagctggg gcgcaaaaag tatgaacgac
    28201 tggagatgcc ctacccgatt gttgaagacc cgtcatacag cgtggaagag ctggacaaac
    28261 tggaggcgtt tttcgcccag ttcgattttg atattcgctt agttcgtcat taaaggagcc
    28321 gtaaatgaac agtttaggcg taattgaaac acgtggtctt gtagccgcaa tccaggccgt
    28381 agatgccgcc tgcaaagcag ccggggtgac ctgtattggt tatcgcaaag taggttccgg
    28441 gctggtaacc gtctgttttg atggggaaat cagcgcagtt tacacggcca ttgaacgtgg
    28501 tattgctgtg gcatccgcaa ccgaccatca ggccaaatca ctggtgatcg ctcgcccgga
    28561 acgctgcgtg gttgaggctc tcagcaacct gaaaggtcat ccgccacgtg tcgaatcagg
    28621 ccagaaacct catgcagagc tgccgacagt tgtagaaatg gctgtctgcg aggaaaccgc
    28681 tgaagtttct acagatactg aaactgcaat gcctgtacct gagaaagaag tgcaagcccc
    28741 catcgtggaa gtgaaaagcc acgggcataa gaaagggaag aaagcatgat tgacgcactg
    28801 ctacaggaaa aaatcaccac gcgactgatc gatactgctc ccactattcc ggtcggtgtt
    28861 tctaatcgtc atgtgcatct gtcgcagcag gatgttgaag cactttttgg tgttggctac
    28921 caacttacgc cctttaagcc gttgcgccag cctgggcaat tcgccgctga agagtgtgta
    28981 acggttgtcg gcccgaaagg ttctctgact cacgtccgcg tacttggacc ggttcgcccg
    29041 acaagccagc tggaaatctc acgtgccgat tgttttactc tcggcattaa agcgccagtg
    29101 cgtgaatccg ggcaactgga aaatgccggt gacgcactgt tggtggggcc gaaaggtcat
    29161 gttgagttgc attcacaagt catttgcgcc tggcggcata ttcatatgtc tccccaggat
    29221 gctcgtctgt tgaatgtcag caatgggcag aaggtgagtg ttcgcagtca cggtgaacgc
    29281 cagttaacgt tcgatgaagt ggtggtacgg gtgcgcaatg attttgccct tgaactgcat
    29341 atcgatacgg aagaagccaa tgctgccgga ctgaaaaacg gggcgcaggt aacactgatt
    29401 cgctaaggga ggccatcatg accgaacaac agatcgacac catcgtcaac ctgatcctcc
    29461 agcggctgca acctgcggta ctggtcatgg tgacatctgc ggatggctat cgggatctga
    29521 ttcatcagcg gttggctcgt tgtggcgaac ggttgcatct ggcgctggat gaaacaatca
    29581 gtgatagcga acggtggcag caaattggtg acgtgatacc cgctaaaaca tggcaacaca
    29641 aattgccttc aacgccctat aaagcgttat tgctgccgtt tttgagttac ccgctggctg
    29701 tagatattgt caacggtacg ctgcaaagcc cggtagcgca aagggttcac gatgcgctac
    29761 tggcgggtat tccggtactg gcactgcgtt attactgcga tccgcacagc gaattaaacg
    29821 cgcttcgcgg tactgttcac agcgactacg cggcgcatct ttccgccacg cttaccggcc
    29881 ttagcgagtg tggcattacg ttatgttcca tgaatgaaat gctggaaaaa ctggcaacag
    29941 acgtcagcag tcaatcgcct gtaagtcatc atcggcgtta tctgactgtc accgatatcg
    30001 tcaataatcc ggcactggca aaatcacccg acgccgtact gaccgatgct gcggtcgatt
    30061 ttttgaaagc acagaaaaaa taaccttacc tcgataacaa acctggagtt tagatatgtc
    30121 ttcgaaaaca aaatgctggc tatggatgtt actggtcatc ctttctgaaa cctctgcaac
    30181 atccacactt aaaatgttcg ataacagtga ggggatgaca aaaacgctgc tgctggccct
    30241 aatcgtcgta ctgtattgca tttgttacta ctcgctttca cgggcagtaa aagacatccc
    30301 cgttggtctg gcttacgcca catggtccgg tactggcatt ttgatggttt caacccttgg
    30361 gattttattt tacggtcaac acccggatac cgccgccatt attggtatgg tcatcatagc
    30421 cagcggtatt atcattatga atctgttctc aaaaatgggc agtgaagagg cggaagaaac
    30481 tccagttacc aacctcgata aaaaaatcgc taactgacaa aaggaattgc tcatgtttaa
    30541 tattggattt ttatggctgg cactttccat cggttctgaa ataaccggta cgtcaatgat
    30601 caaaaaaacc aatggtttta ggcgactgtg gccttctgtt ctggtggtat gtgcttattc
    30661 aacgtgttat ttcgctctga ccagagcaat gagtactatt ccggttggtg ttgcctattc
    30721 actctggtgc ggctttggga ttgttggcgt gacaattttc tcgatgattc tttataaaca
    30781 aaagcccgat ttacctgcca tattctccat gttgctgatt attagtggcg gcattattat
    30841 gaatgtattt tcaaacatgt aatgacccca aaagtctgct ttcacgcaca atgcggatca
    30901 gttaaggagc agttgaccgg tccttaaact gcggcactat agcggcttcc acaacaggga
    30961 gccgttttct tgtgcacagc ccccagctag gctctgtctc ttatacacaa atccccaccc
    31021 ggggatttgt gatgtctgtc agttgaaccg ccccgtgttt cctggagagt gttttatctg
    31081 tgaactcagg ctgccagctc atcatttccg atggaagcat aatacgcttt ttctgcctct
    31141 gacggaggga tgtggcccag ccttaccagt aagcatcgat tgttatacca gtccacccac
    31201 gtgagtgtga ccagttccac ttctgcacgg tttttccagc tcttacggtg tattacctcc
    31261 gctttgtaaa gaccattgat gctctccgcc atcgcgttgt catacgagtc gcctgtactt
    31321 cctgttgatg ccagcagttt tgcttctttt aatcgctccg tataggccag tgacacatac
    31381 tgagagcctt tatcgctgtg atggatggtg ccggacggac gatgggccca caacgcctgc
    31441 tccagcgcat ccagcacgaa tgtcgtttcc atggacgatg agacccgccc ccccacgatg
    31501 tatccggcaa acacatcaat gatgaacgcc acatagacga agccctgcca tgtgctgagt
    31561 agagtaagag gcagggcgta gtcggactat ttccctgcct ctcctccccg aaccggacgt
    31621 gcacctttca gcgcatccgg ctctccattt aaatgctggc gaac
//
LOCUS       DAOTRK010000046        31518 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.046, whole genome shotgun sequence.
ACCESSION   DAOTRK010000046 DAOTRK010000000
VERSION     DAOTRK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.046"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..570
                     /gene="tuf"
                     /locus_tag="QIE41_002023"
     CDS             <1..570
                     /gene="tuf"
                     /locus_tag="QIE41_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDS3530913.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIH
                     PIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            800..1183
                     /gene="secE"
                     /locus_tag="QIE41_002024"
     CDS             800..1183
                     /gene="secE"
                     /locus_tag="QIE41_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDS3530914.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1185..1730
                     /gene="nusG"
                     /locus_tag="QIE41_002025"
     CDS             1185..1730
                     /gene="nusG"
                     /locus_tag="QIE41_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDS3530915.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1889..2317
                     /gene="rplK"
                     /locus_tag="QIE41_002026"
     CDS             1889..2317
                     /gene="rplK"
                     /locus_tag="QIE41_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDS3530916.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2321..3025
                     /gene="rplA"
                     /locus_tag="QIE41_002027"
     CDS             2321..3025
                     /gene="rplA"
                     /locus_tag="QIE41_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDS3530917.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3438..3935
                     /gene="rplJ"
                     /locus_tag="QIE41_002028"
     CDS             3438..3935
                     /gene="rplJ"
                     /locus_tag="QIE41_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDS3530918.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4002..4367
                     /gene="rplL"
                     /locus_tag="QIE41_002029"
     CDS             4002..4367
                     /gene="rplL"
                     /locus_tag="QIE41_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDS3530919.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4687..8715
                     /gene="rpoB"
                     /locus_tag="QIE41_002030"
     CDS             4687..8715
                     /gene="rpoB"
                     /locus_tag="QIE41_002030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDS3530920.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8792..13015
                     /gene="rpoC"
                     /locus_tag="QIE41_002031"
     CDS             8792..13015
                     /gene="rpoC"
                     /locus_tag="QIE41_002031"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDS3530921.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13228..13767
                     /locus_tag="QIE41_002032"
     CDS             13228..13767
                     /locus_tag="QIE41_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HDS3530922.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARIHLKRTILALPES
                     NVIHAGYGSYAVIEVDLNGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14177..15310)
                     /gene="thiH"
                     /locus_tag="QIE41_002033"
     CDS             complement(14177..15310)
                     /gene="thiH"
                     /locus_tag="QIE41_002033"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDS3530923.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(15307..16077)
                     /gene="thiG"
                     /locus_tag="QIE41_002034"
     CDS             complement(15307..16077)
                     /gene="thiG"
                     /locus_tag="QIE41_002034"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDS3530924.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAIADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16079..16279)
                     /gene="thiS"
                     /locus_tag="QIE41_002035"
     CDS             complement(16079..16279)
                     /gene="thiS"
                     /locus_tag="QIE41_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDS3530925.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(16263..17018)
                     /gene="thiF"
                     /locus_tag="QIE41_002036"
     CDS             complement(16263..17018)
                     /gene="thiF"
                     /locus_tag="QIE41_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDS3530926.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINTACVALNTPLITASAAGFGGQLMV
                     LTPPWEQGCYRCLWPDTQDPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRATGCPVCGGSNADPV"
     gene            complement(17011..17646)
                     /gene="thiE"
                     /locus_tag="QIE41_002037"
     CDS             complement(17011..17646)
                     /gene="thiE"
                     /locus_tag="QIE41_002037"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDS3530927.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSAQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAITLGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVMATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17646..19541)
                     /gene="thiC"
                     /locus_tag="QIE41_002038"
     CDS             complement(17646..19541)
                     /gene="thiC"
                     /locus_tag="QIE41_002038"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDS3530928.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGLLAPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19622..19722)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19775..20251)
                     /locus_tag="QIE41_002039"
     CDS             complement(19775..20251)
                     /locus_tag="QIE41_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HDS3530929.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLVRAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20346..21119
                     /gene="nudC"
                     /locus_tag="QIE41_002040"
     CDS             20346..21119
                     /gene="nudC"
                     /locus_tag="QIE41_002040"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDS3530930.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21159..22223
                     /gene="hemE"
                     /locus_tag="QIE41_002041"
     CDS             21159..22223
                     /gene="hemE"
                     /locus_tag="QIE41_002041"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDS3530931.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22233..22904
                     /gene="nfi"
                     /locus_tag="QIE41_002042"
     CDS             22233..22904
                     /gene="nfi"
                     /locus_tag="QIE41_002042"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDS3530932.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22947..23537
                     /locus_tag="QIE41_002043"
     CDS             22947..23537
                     /locus_tag="QIE41_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDS3530933.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23724..23996
                     /gene="hupA"
                     /locus_tag="QIE41_002044"
     CDS             23724..23996
                     /gene="hupA"
                     /locus_tag="QIE41_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDS3530934.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24009..24704
                     /locus_tag="QIE41_002045"
     CDS             24009..24704
                     /locus_tag="QIE41_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDS3530935.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24706..25131)
                     /gene="zraP"
                     /locus_tag="QIE41_002046"
     CDS             complement(24706..25131)
                     /gene="zraP"
                     /locus_tag="QIE41_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDS3530936.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25369..26766
                     /gene="zraS"
                     /locus_tag="QIE41_002047"
     CDS             25369..26766
                     /gene="zraS"
                     /locus_tag="QIE41_002047"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDS3530937.1"
                     /translation="MRFMQRSKDTLAKWLSAILPVVIVGLVGLFAVTVIRDYGRASEA
                     DRQALLEKGNVLIRALESGSRVGMGMRMHHVQQQALLEEMAGQPGVLWFAVTDAQGII
                     ILHSNPEMVGRALYSPDEMQQLKPEENSRWRLLEDAEKTPALEVYRLFQPMSLPWRHG
                     MHNMPRCNGKAVPQAEAQQAIFIAVDASDLVATQSGEKRNTLIILFALATVLLASVLS
                     FFWYRRYLRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPA
                     GGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANS
                     REIQLRFTANDMLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKIS
                     VTDSGKGIAADQLEAIFTPYFTTKADGTGLGLAVVHNIVEQHGGTIQVASQEGEGATF
                     TLWLPVNITRKDPQG"
     gene            26763..28088
                     /gene="zraR"
                     /locus_tag="QIE41_002048"
     CDS             26763..28088
                     /gene="zraR"
                     /locus_tag="QIE41_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDS3530938.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGQQALAQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHMHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28085..29374)
                     /gene="purD"
                     /locus_tag="QIE41_002049"
     CDS             complement(28085..29374)
                     /gene="purD"
                     /locus_tag="QIE41_002049"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDS3530939.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GNKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVSDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29386..30975)
                     /gene="purH"
                     /locus_tag="QIE41_002050"
     CDS             complement(29386..30975)
                     /gene="purH"
                     /locus_tag="QIE41_002050"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDS3530940.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7448 a         8141 c         8624 g         7305 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggcc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      421 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      481 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      541 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      601 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      661 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      721 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaat
      781 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      841 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtagcgattg tcggcaacta
      901 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      961 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct accgttgctt ttgcccgtga
     1021 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
     1081 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
     1141 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
     1201 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
     1261 tgcgtgagca tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa
     1321 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1381 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1441 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcacca atcagcgata
     1501 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1561 cgctgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1621 gtgttgttga agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1681 gtcgtgcgac cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc
     1741 aaaaaagcgg cgatttaatc gttgcacaag gcgcgagatt ggaatacaat ttcgcgcctt
     1801 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1861 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1921 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1981 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     2041 aggtctgccg attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac
     2101 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     2161 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     2221 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     2281 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     2341 catgcgtgtt atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2401 cgctctgctg aaagaactgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2461 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2521 cggtactggc cgttccgttc gcgtagccgt atttacccag ggtgcaaacg ctgaagctgc
     2581 taaagctgca ggtgctgaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2641 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2701 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2761 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2821 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2881 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2941 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     3001 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     3061 tacccccacg tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     3121 cggcaactga gtgagtcgcc aggagcatag ctaactatgt gactggtgcg aatgaaggaa
     3181 gccaacgccg ccacaagctg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     3241 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     3301 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3361 ttaagacgct ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3421 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3481 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3541 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3601 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3661 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3721 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3781 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3841 caattgcacg cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc
     3901 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3961 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     4021 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     4081 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     4141 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     4201 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     4261 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     4321 tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4381 gttgcagcct gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg
     4441 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4501 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4561 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4621 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4681 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4741 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4801 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4861 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4921 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4981 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     5041 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     5101 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     5161 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg tatcatccct
     5221 taccgtggtt cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatt
     5281 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgtg ccctgaacta caccacagag
     5341 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5401 atggaactgg tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac
     5461 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5521 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5581 aaagactata ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc
     5641 ctggatctgc tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc
     5701 aacgacctgg atcacggccc gtatatctct gaaaccttac gtgtcgaccc aactaacgac
     5761 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5821 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgcta tgacctgtct
     5881 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5941 atcctgagca aagacgacat cattgatgtt atgaaaaagc tcattgatat ccgtaacggt
     6001 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa
     6061 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     6121 ctgtctctgg gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt
     6181 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     6241 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc aggtggtctg
     6301 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta
     6361 tgtccaatcg aaacccctga aggtccgaac atcggtctga tcaactctct gtctgtgtac
     6421 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt
     6481 gtaactgacg aaattcacta cctgtctgct atcgaagaag gcaactacgt tatcgcccag
     6541 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ttgccgtagc
     6601 aaaggcgaat ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag
     6661 caggtggtat ctgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6721 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgtgc tgataagccg
     6781 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggttgct
     6841 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6901 gacgagatgt atccgggtga agcaggtatc gacatctata acctgaccaa atacacccgt
     6961 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggtagaa
     7021 cgtggcgacg tgctggcaga tggtccgtct accgacctcg gtgaactggc gctgggtcag
     7081 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     7141 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     7201 gtgtcccgtg acaccaagct ggggccggaa gagatcaccg ctgacatccc gaacgtgggt
     7261 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtaacc
     7321 ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa
     7381 gaaaaactgc tgcgtgcgat cttcggtgag aaagcgtctg acgttaaaga ctcttctctg
     7441 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7501 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7561 gacctgtctg aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg
     7621 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7681 gagctgggcc tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7741 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7801 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc
     7861 cgtatccagc ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag
     7921 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7981 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     8041 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     8101 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     8161 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     8221 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     8281 ctgaaacttg gcgacctgcc gacttctggt cagatccgcc tgtacgacgg acgcactggt
     8341 gaacagttcg aacgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8401 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8461 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8521 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8581 aacggtcgta ctaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8641 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8701 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8761 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8821 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8881 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8941 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     9001 cctgtgcggt aagtacaagc gcctgaaaca ccgtggtgtc atctgtgaga agtgcggcgt
     9061 tgaagtgacc cagactaaag tacgccgtga gcgtatgggt cacatcgaac tggcttcccc
     9121 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggcctgc tgctcgatat
     9181 gccgctgcgc gatatcgaac gcgtactgta cttcgaatcc tatgtggtta ttgaaggcgg
     9241 tatgaccaac ctggaacgcc agcagatcct gactgaagag cagtatctgg acgcgctgga
     9301 agagttcggt gacgaattcg acgcgaaaat gggtgcggaa gcaatccagg ccctgctgaa
     9361 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9421 cgaaactaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9481 tggtaacaaa ccagagtgga tgatcttgac cgttctgccg gtactgccgc cagatctgcg
     9541 tccgctggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9601 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9661 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9721 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgatat
     9781 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9841 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9901 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9961 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
    10021 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
    10081 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
    10141 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt
    10201 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
    10261 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct
    10321 gtactacatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10381 gaaagaagca gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt
    10441 gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa
    10501 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggcc tgccttactc
    10561 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10621 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc
    10681 ctatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10741 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10801 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10861 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga
    10921 cggccaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10981 gcgtggttct gcggcacaga ttcgtcagct tgcaggtatg cgtggtctga tggcgaagcc
    11041 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    11101 ccagtacttc atctccaccc acggtgcgcg taaaggtctg gcggataccg cactgaaaac
    11161 tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    11221 cgaagacgat tgtggtaccc atgaaggtat catgatgact ccggttatcg aaggtggtga
    11281 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    11341 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11401 cctgctggaa gaaaactctg tcgacgcggt taaagtacgt tctgttgtat cttgtgacac
    11461 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcattaa
    11521 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11581 gaccatgcgt acgttccaca tcggtggtgc ggcatcacgt gcggctgctg aatccagcat
    11641 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11701 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11761 taccaaagaa agctacaaag taccttacgg tgcggtactg gcgaaaggcg atggcgaaca
    11821 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11881 agtaagcggt tttgtacgct ttactgacat gatcgacggc cagaccatta cgcgtcagac
    11941 cgacgaactg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    12001 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat
    12061 ccctggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    12121 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatccggcgg
    12181 taccaaggac atcaccggtg gtctgccgcg cgttgcggat ctgttcgaag cacgtcgtcc
    12241 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    12301 aggtaaacgt cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat
    12361 tccaaaatgg cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12421 ttccgatggt ccggaagcgc cacacgacat tctgcgtctg cgtggtgttc atgctgttac
    12481 tcgttacatc gttaacgaag tacaggacgt ataccgtctg cagggcgtta agattaacga
    12541 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12601 cagctccgac ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12661 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12721 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12781 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12841 cgttatcgtg ggtcgtctga tcccggcagg tactggttac gcgtaccacc aggatcgtat
    12901 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12961 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    13021 aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggggt agtttaggga
    13081 aagagcattt gtcagaatat ttaatgaatt tctggatact cataatcaat gtagagatga
    13141 ctaatatcct gaaactgact gaactaattg agtcaagctc ggcaagaatt cgatactatt
    13201 cctgtgtgac tttcttaagg aacgataatg aaacaggaag tggagaagtg gcgacctttt
    13261 ggacatccgg atggtgatat tcgtgattta tcatttcttg atgctcatca ggctgtctac
    13321 gttcagcatc atgagggcaa agagccttta gagtatcgct tctgggttac ctactctctt
    13381 cactgcttca caaaagatta tgaacatcag actaacgaag aaaaacaatc gttaatgtac
    13441 cacgcgccta aagaatctcg tcccttctgc cagcaccgtt ataacttagc gcgcatacac
    13501 ttaaaaagaa ccattttggc gctgccagaa agcaacgtta ttcatgctgg gtatggtagc
    13561 tatgccgtga ttgaagtgga tttaaacgga ggagacaagg cattttactt tgttgcgttc
    13621 agggctttca gggaaaagaa aaaacttcgt ttgcatgtaa ctagcgctta tcccatttct
    13681 gaaaaacaga aaggtaaatc agtgaaattt ttcaccattg cctacaactt attgagaaat
    13741 aagcagcttc ctcagccctc aaaataacaa aacccacctt aaggtgggtt tcgccagaga
    13801 attatctctg gtattcagaa cgccattacc ggactttgcc ttgaccttgc gataatcgca
    13861 ggttgcggga tgtctgaatt tcttcagtct gctgcatcct ggaagatgag aacatgtgtt
    13921 cttattttcg tctctatcat agttgagtat ttactctctt acaatcagat ctctttcatt
    13981 gctcaacagg cgatggcttc agactttgca ttacggaatt tttaagaaag gcagggcgaa
    14041 acgaggaaga agcttttcgc cctggacaac attcctgctg acggcactac cataaaaaaa
    14101 cacaacgctt tcaacaacct tgcgtttttc atcttccgta gcggctcgga ataaatttac
    14161 gttcttacat tccgtctcat ggcctttgcg aggcgcgtcc cagatagctg tcccagtctt
    14221 tccataccgg ctgcaaaccc tgagcggtta acgcggcagc aaccgcttcc ggtctgcgat
    14281 cgtcgtgtgg tgagaactgt tccagctcgg ggtgattgtc ggcatagcca cctggctgcg
    14341 ttttcgagaa agcgctgacg ttattaattg ccagcggaat cacgcgatcg cgaaaccacg
    14401 gtgattcccg cgtggagagt gacagttcaa tctccggtgc aagcagccgg aaggcgcaga
    14461 tggtttgcac taactggcgt tcatccataa tcgacgcagg ctcaatgccg ccagtacatg
    14521 gacgcaggcg tgggaaggag acagagtagc ggctttgcca gtaatgctgt tgcagccata
    14581 gcaaatgttc tgcaaccata tagcagtcaa cgcgccagct gtcggaaagg ccaattagcg
    14641 cgccgaggcc tatcttatca atccccgcac ggcccagccg atccggcgtt tccagccgcc
    14701 agaagaagtc ctgttttttg cctttcagat ggtggcgggc ataagtcgcc tcgtgatatg
    14761 tctcctgata aaccatcacg ccatccagac caagttgctt taactcggcg tattccgtct
    14821 ccgccagcgg ctgtacttcc atctgtagtg aagagaactg ttcacgcagg gcagggagat
    14881 gacgacgaaa gtaatccatc cccactttcg cctgatgttc accagtgact aaaagcagat
    14941 gttcaaagcc catctcccgt atagcggcgc tttccctggc aatatccgct tcatccagcg
    15001 ttttgcgctt gatgcggttg ctcatagaga atccgcagta cgtgcagtcg ttagcgcaaa
    15061 gattggaaag ataaagcggg acgtagaagc taacagtgtt gccaaatcgt tgacgggtca
    15121 gacgctgcgc ccgttgggcc agtggttcca gatagccact ggcggctggc gataacagcg
    15181 ccatcatatc gtcgcgggtg agttgcgagg catttagcgc ccgctctacg tcagcagccg
    15241 ttttgctgtt gatacgcagg cggatgtcgt cccagtccag ttgtcgccag cgatcgctga
    15301 aggttttcat gccgatgcct ccagaaatcc ggtcagcggg ctggtggcat gagcaaaatg
    15361 actgcggctg cccggtccgg actgacgtgc cagcaggcct gcttctactg ccagacgaaa
    15421 tgccttcgcc atgttgacgg gatcgtccgc gatggcaatc gccgtattca ctaacaccgc
    15481 gtcggccccc atttccagcg cctgcgcggc atggctggga acgccgatgc cagcgtcgac
    15541 aaccaccgga accgtggctt gctggataat aatctccagc atggcacggg tttccagtcc
    15601 ctgattcgag ccaatcggcg cgccgagcgg catcaccgct gcacagccga cttcttccag
    15661 acgtttacac aataccggat cggccccgca gtaaggcagc acgacaaatc cctgttgtac
    15721 cagcgtttcg gcggctttca gggtttcgat gggatcgggc aacagccagc gggcgtcagg
    15781 gtgaatctct aattttagcc agtttgtgcc taatgcttca cgtgccagat gggcggcgaa
    15841 aatggcttct tccgctgttt tcgccccgga tgtattcggt agcagggtca cacccgccgc
    15901 gataagcggt tcgaggatag cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag
    15961 cgtcaccagt tggctgccgg aagcgcgaat agactccacc atcagttgtg atgaggcgaa
    16021 tttcccggtg ccggtaaaca gatgtgaatc aaacgttttg tccgcaatac gtaacatctc
    16081 aaccccctgc aataacctga aaaagcagga tctggtcgcc atcctgcacg atatgttgcg
    16141 cccactgctc acgcgggacg atttgctgat taatcgccag agccgcgccc gcttgtcgtt
    16201 ggtcgagttg ctccagtagt tcgtgaaccg tttgcccggc ggcgcactgc atcggttgat
    16261 cgttaaacag gatctgcatt gcttcctccg cataccgggc aacctgtagc gcggcgcagc
    16321 gccaggctgc gccactggct agacttaccg tcgaacagtc ggagttctcc cgcaggcgtc
    16381 tctataccgc ttaacaactt aatggcttcc agtgcctgca aagtgcccat aaccccgacc
    16441 accgggccaa ccacgcccgc cgtgcggcag ttgcgttctg gatcctgggt atctggccac
    16501 aggcagcggt aacacccctg ctcccagggc ggcgtcagta ccatcaactg accgccaaat
    16561 ccggccgcgc tggcggtgat aagcggcgtg ttgagtgcca cgcaggcggt attaatctcc
    16621 tgacgagtcg ccatattgtc ggtacagtcg agcaccacat cggcccgtgc taccgcatct
    16681 tttaacgcct cacccgttaa ccgttgttgt aatgctgtca gttgaatatc gggattcaac
    16741 tgtgtcagtc gctgatggct gacctgcgat ttcgggcgat cgatatcttc agtggtaaag
    16801 aggatttgtc gttgcagatt gcttaaatgc acatcgtcgt catctgccag taccagcgtc
    16861 ccgacgccag cgcccgccag gtatagcgca gcaggtgtac ccagcccgcc aagaccaata
    16921 atcagcacct ggctgtcgag cagtttttgc tgcccgtcaa gagcgatatc gtcgagcagg
    16981 atttggcggc tataacgcat aaagtcacgg tcattcatcg ccaactcctg caatttccag
    17041 caactgtgcc gttgccaaac gccagtctgc ggcttgagta atggcgctga caacggcgat
    17101 actgccgaca cccgtcgcca ttaccgcagg cgcgcgcgcc agactgatac cgccaatcgc
    17161 cacggtggga taatccgcca ggcgctcaac atgccgtgcc agctgttcca gcccctgcgg
    17221 tgcagaaggc atctgtttgg tttgcgtcgg gaacacatgt cccagcgcga tataagaggg
    17281 gcgtgctgcc agcgcgacgt cgatctccat atcgtcatgg gtcgaaacgc ccagtcgcag
    17341 acctgccgcg cggatggcgc tgagatcggt ggcttgcaaa tcttcctgcc ccagatggac
    17401 gccatacgcc tgatgcttga tcgccagccg ccagtaatcg ttgataaaca atcgcgcgtt
    17461 atagcggcgg cccagcgtaa ttgccgccac gacatcggct tccacctctt catcgcgccg
    17521 atctttgatg cgtagctgga gagtacgcac gcctgcatcc aacagacgtt cgatccactg
    17581 tgcgctgtcc accaccgggt acagtcctaa acgaaaaggt acaggaggaa aatcaggctg
    17641 atacatcacg cttcctcctt acgcaggtag atttctccgc ctctggcgcg gaagttctcc
    17701 gacatatccg ccattcccac ttcaatggtt tgcgcggcgg cgtaatcacg cacttcctgg
    17761 ctgattttca tcgagcagaa tttcggccca cacatggagc aaaaatgggc gactttaccg
    17821 gactcttgcg gcagggtttc atcgtgataa gcgcgggcag taaacgggtc gagggccaga
    17881 ttaaactggt cttcccagcg aaattcgaag cgggctttcg acatggcgtt atcgcgaatt
    17941 tgcgcgcccg gatgcccttt cgccaggtca gcggcgtgcg cggcaatctt ataggtgata
    18001 agcccctgct taacatcttc tttattaggc agacccagat gctcttttgg cgttacgtaa
    18061 cagagcatcg cgcagccaaa ccagccaatc atcgccgcac caatccccga cgtgaagtgg
    18121 tcataacccg gcgcgatatc ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg
    18181 cagtgctcta actcctcggt catattgcgg cggatcatct gcatcggcac gtggcctggg
    18241 ccttcaatca tcacctgtac gtcatattcc caggcgattt tggtcagttc acccagcgta
    18301 tgcagctcgg caaattgcgc ttcatcgttg gcgtcctgaa cagaaccggg gcgcagaccg
    18361 tcgcccagcg acagcgaaac gtcataagcg gcacagattt cacaaatttc gcggaagtgt
    18421 tgatagagga aattttcctg atgatgggag aggcaccatt tcgccataat cgaaccgccg
    18481 cgagagacga taccagtcag gcgtttcgcg gtcatcggca catagcgcag cagtacgccc
    18541 gcatggatag tgaagtaatc cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac
    18601 gcttcccagg taagatcttc ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc
    18661 actgtaccga tcggcaccgg gctgttacgc aaaatccact cgcgggtttc gtgaatatag
    18721 cgaccggtgg agagatccat caccgtatcc gctccccagc gcgtggacca taccagcttt
    18781 tccacttctt cttcgatgga agaggtgacc gccgagttgc cgatattggc gttaactttt
    18841 accaggaaat tgcgaccaat aatcatcggc tccgattccg gatgattaat gttggccggg
    18901 ataatcgcgc gtccggcagc aacttcatca cggacaaatt ccgcagtgat attttccggc
    18961 agacgtgcgc caaagctcat tcccggatgc tggtggcgta aaacttcgct acgtatgcgc
    19021 tcgcggccca tattctcgcg gatggcgatg aattccattt ccggtgtgat gatgccctgg
    19081 cgggcgtagt gcagttgggt gacgcggcgt cctgctttgg cgcgttttgg cgctaataag
    19141 ccgctaaaac gtagctcgtc gaggccatca tctgccagtc gcgctttagt gtaatcggaa
    19201 ctgcgcacgg taagttcttc ggtatcgccg cgcgcatcga tccacggctg gcgtagtttt
    19261 gccagtcctt gctgcacgtt aatggcgatc tgcggatcac catacgggcc ggaggtgtcg
    19321 tagaccggaa tcgcttcgtt ttcttcaaac tgcggctgtt ctttgctacc gccaattagc
    19381 gtcgggctaa gctggatctc acgcatcggc acgcgcacgc cgggttgtgt gccagtgata
    19441 taaatgcgtt ttgagttggg aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc
    19501 tgggcgcgtt gttcgcggcg ggtcagtttt gttgcagaca tagctcattc caaaaagtta
    19561 aggacgtggc ttgtcagacg acggatgaag caagagacga tcgccgcaaa ggcgatgcga
    19621 tagcagatta actcttgttc ccttcgcagg tattagcctg atcaggttcc gcggatcccg
    19681 aataaacggt ctcagccagt taaggcactc cgacaagaaa ttagccccca caaacggggc
    19741 gttgaatgta aattacgcgt taacagcgca gaactcaagc aggatgtttg acgcgggcgg
    19801 cgtcaagcac cagcagaatc agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc
    19861 cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc atgatcgatg gcggtttcca
    19921 ggctggaatc gtagtaatcc ataatctgtt gggtattggc ttcgagttgc ggccaaatct
    19981 tcgcggcgcg cacgagttgc ccgttgcctt ccagcttatg aagaatgcgc tcataaatac
    20041 tgaaatgtcc ggcagacaag taatcgacca ggctctgaca aaaatcatca agggcttttt
    20101 cgtttagcct catgtacgat tctttgccag gcttaatgcc aaccagattg tagtaagcca
    20161 cgagcagatg cttacgtaca tgtagccagc gatcaaccag tttgttactt cctctgacgc
    20221 gttccgtcag gttatcgagc tggttaagca tgattgactc cgcaagtttg tattcaaaaa
    20281 ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa
    20341 gagctatgga tcgtataatt gaaaaattag atcacggctg gtgggtcgtc agccatgaac
    20401 aaaaattatg gttgccgaag ggagaattgc catatggcga agcggcaaat ttcgatcttg
    20461 tgggtcagcg cgcactacaa atcggcgaat ggcaggggga acctgtttgg ttagtacaac
    20521 agcagcggcg tcacgatatg gggtcggtac gtcaggtcat tgatctcgat gttgggctgt
    20581 ttcaactggc cggacgaggc gtacaactgg cggagtttta ccgatcgcat aaatactgtg
    20641 gttactgcgg gcatgaaatg tatccgagca aaaccgaatg ggcgatgctg tgcagccatt
    20701 gccgtgagcg ttactatccg caaatcgccc cctgcattat tgttgccatc cgtcgcgatg
    20761 attcgatcct ccttgcccag catacccgcc atcgtaacgg tgtccatacg gtacttgccg
    20821 gattcgtcga agtgggcgaa accctcgagc aggcggtcgc gcgggaagtg atggaagaga
    20881 gcggaattaa agttaaaaac ttgcgttatg tgacttccca gccgtggccg tttcctcagt
    20941 ctttaatgac cgcgtttatg gcggaatacg acagcggcga gatcgttatc gacccgaaag
    21001 aattgctcga ggcgaactgg tatcgctatg acgatttgcc gttactcccg ccgcccggca
    21061 ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg tcgggcagag tatgagtgat
    21121 gatacactga ccgcctgacg cactaaggaa cagccaaaat gaccgaactt aaaaacgatc
    21181 gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc
    21241 aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc ccaggcgggc gattttatgt
    21301 cactgtgcaa aaacgccgag ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctacc
    21361 cgctggatgc ggcgatcctc ttttccgata tcctcaccgt gccggacgcg atggggttag
    21421 ggctctattt tgaagccgga gaaggtccgc gttttacctc gccagtcacc tgcaaagccg
    21481 acgtcgataa actgccaatt ccggacccgg aagatgagct gggttacgtg atgaacgcgg
    21541 tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct gattggtttt tccggcagcc
    21601 cgtggacgct ggcgacctat atggtggaag gcggcagcag caaagccttc accgtgatca
    21661 aaaaaatgat gtatgccgat ccgcaggcgc tgcacgcgct actcgataaa ctggcgaaaa
    21721 gcgtcacttt gtatctgaat gcgcagatta aagccggtgc tcaggcagtg atgattttcg
    21781 acacctgggg cggcgtgctt accgggcgcg attatcaaca gttctcgctt tattacatgc
    21841 ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg ccgcgtaccg gtcacgctgt
    21901 ttaccaaagg cggcggacag tggctggaag ccatggcaga aaccggttgc gatgcgctgg
    21961 gcctcgactg gacaacggac atcgccgatg cgcgccgccg cgtgggcaat aaagtcgcgt
    22021 tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc agcccgcatt gaagaagaag
    22081 tagcgactat acttgcaggt ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg
    22141 gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt tgtggaggca gtgcatcgct
    22201 tgtctgaaca gtatcaccgc taaggagcga ttatggatct cgcgtcatta cgcgctcaac
    22261 aaattgaact ggcttcttct gtgatccgcg aggatcgact cgataaagat ccgccggatc
    22321 tgatcgccgg agccgatgtc ggatttgagc agggcggaga agtgacgcga gcggcgatgg
    22381 tgctgctgaa atacccctcg cttgagctgg tggagtataa agtggcccgc atcgccacca
    22441 ccatgcctta cattccaggt tttctttcct tccgcgaata tcctgcgctg ctggcagcgt
    22501 gggagatgct gtcgcaaaag ccggatttag tgtttgtcga tggtcacggg atctcgcatc
    22561 ctcgccgtct tggcgtcgcc agccactttg gtttgctggt ggatgtgccg accattggcg
    22621 tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc cagcgaaccg ggcgcgctgg
    22681 ccccgctgat ggataaaggc gagcagctgg cctgggtctg gcgcagcaaa gcgcgctgta
    22741 acccgttgtt tatcgctacc ggccatcggg tcagcgtaga cagcgcgctg gcgtgggtac
    22801 aacgctgcat gaaaggctat cgtctgccgg agccaacgcg ctgggcggac gcggtggcct
    22861 cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc ctaattcagg taaactgcgg
    22921 ataatttccg tatttgagaa ctcaacatgt tacaaaaccc aattcatctg cgtctggagc
    22981 gcctggaaag ctggcagcac gtcactttta tggcctgctt atgcgaacgc atgtacccta
    23041 attacgccgt attctgtcag caaactggtt ttggtgatgg gcagatttac cgccgcattc
    23101 tcgatctcat ctgggaaacg ctgaccgtca aagacgcaaa agtaaatttc gacagccaac
    23161 tggagaaatt tgaagaagcg attccttcag ccgacgattt cgatctgtac ggcgtttatc
    23221 cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca ttcgcgtttg agtggtgaaa
    23281 cgctcgaaca cgcagtggaa gtgagtaaga cctccatcac gaccgttgcg atgctggaaa
    23341 tgactcaggc tggtcgcgaa atgagcgatg aagagctcaa agaaaaccca gctgtagagc
    23401 aagaatggga cattcagtgg gaaatattcc gacttttagc tgagtgcgaa gaacgcgata
    23461 tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc gggcgagagc aatattggta
    23521 taatttttca gcaataagac cagaaaacgt gatttaacgc ctgatttgtc gtacctggag
    23581 tcttcccttt cgccccccgt ctggtctaca tttggggggc gaaaaaaagt ggctatcggt
    23641 gcgtgtatgc aggagagtgc tattctggca tttccgtcgc actcgatgct tagcaagcga
    23701 taaacacatt gtaaggataa cttatgaaca agactcaact gattgatgta attgcagaga
    23761 aagcagaact gtccaaaacc caggctaaag ctgctctgga gtccactctg gctgcaatta
    23821 ctgagtctct gaaagaaggc gatgctgtac aactggttgg tttcggtacc ttcaaagtga
    23881 accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg taaagaaatc aaaattgccg
    23941 cagctaacgt accggcattt gtttctggca aggcactgaa agacgcagtt aagtaagatt
    24001 gcgtggcagt gaacagtttt aacgaagggg tggtttcacc ccttttgtct ttctggcgtc
    24061 gatcattgat gctggcaggc gctctgtttc tcactgcctg tagtcacaac tcttcacttc
    24121 ctccctttac cgccagtgga tttgctgaag accagggcgc ggtacgcatc tggcgaaaag
    24181 acagcggcga taatgtgcat ctgcttgccg tgtttagccc gtggcgcagt ggcgatacca
    24241 ccacgcgaga gtatcgctgg cagggcgata accttacgct catcaatatc aatgtttaca
    24301 gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg cggcgatctg agctttatgc
    24361 aacgtgaatc cgatggggaa aagcagcagc tttctaacga ccaaatcgat ttataccgtt
    24421 atcgtgctga tcagatccgc cagattagcg atgctttacg tcaggggaga gtcgtgctgc
    24481 gccaggggcg ctggcatgcg atggaacaga ccgtgaccac ctgcgaagga caaaccatta
    24541 aacctgattt agattcgcag gcgatagcgc atatcgagcg ccgccagagc cgctcttctg
    24601 ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc gcaattactg ttagtggcaa
    24661 actctgattt ctgtcgctgg caacccaacg agaaaacgtt ctgatttacc agtggcccat
    24721 acccatatga ccgccaccac cgcagccgcc gtagcccatg cccattccgg ctccgcgcgg
    24781 aatacccgct tcagccatcg caatatcccg tttcacccgt aactcatcta acgactgacg
    24841 caaattctcc atctctttgg cgaccgcgtt aattttgctg ctatccggtg ggttcgcggc
    24901 taacagggca ttgtattcat aacgcttcgt caccagttgc tgttgcagtg cgctgctttg
    24961 agcgtaaaag tcattatgga ttttctgcca cgctgtctgt tgttcgctgg tcaaaggcgc
    25021 ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat gcagatgtcg atcccatcgc
    25081 cattgctgaa agcgccatca tcaccagggc aattttcgtg ttccgtttca tggttaatcc
    25141 tccagtggtt gtcttacttt gggtattgca tctttcgtgc caacaacgaa acgctgatat
    25201 gacgggtaat ctggcatgat aaacgagtaa aaatgactcg cctgttgtgg gtagcgagtc
    25261 atttttactc attgaaactt aagtctttgt gttacagcgc agggtaagcg ctgctaaaag
    25321 atggcatgat ttctgctgtc aaaaagggat gaacaggcaa agaagaagat acgttttatg
    25381 caacgttcaa aagacacctt agccaaatgg ttaagcgcga tcctccccgt ggtcattgtt
    25441 gggctggtgg gattgtttgc ggtaacggtg attcgtgatt atgggcgggc aagtgaggct
    25501 gaccgtcagg cattactgga aaaaggtaat gtgctcattc gcgctctgga gtcaggaagc
    25561 cgcgtaggga tggggatgcg aatgcaccat gtgcagcaac aggcgcttct ggaagagatg
    25621 gcaggccagc ctggggtgtt gtggtttgcg gtcaccgatg cgcagggcat catcattctt
    25681 cacagtaacc ccgaaatggt cgggcgtgca ctctattctc ctgatgaaat gcagcagtta
    25741 aagccagagg aaaactcccg ctggagactg cttgaggatg cagaaaaaac gcctgcgctt
    25801 gaggtctatc gtttgttcca gccaatgtca ttgccttggc ggcatggtat gcacaatatg
    25861 ccgcgctgta acggcaaagc tgtgccacag gcagaagcac aacaggctat tttcatcgcc
    25921 gttgacgcca gcgatctggt tgcaacccag agtggggaaa agcgcaatac cctgattatc
    25981 ctcttcgccc tggcgacggt cttgctggca agcgtgttgt cattcttctg gtatcgccgg
    26041 tatctgcgct cgcgccagct tctacaagat gaaatgaagc gcaaagagaa gctggtggca
    26101 ctggggcatc tggcggcagg cgttgcccac gaaatccgta acccactttc ctcgattaaa
    26161 gggctggcga aatactttgc cgaacgcgcg cctgcagggg gagaagcgca tcaactggcg
    26221 caagtgatgg cgaaagaggc tgaccgttta aaccgcgtgg taagtgagtt gctggaactg
    26281 gttaagccaa cgcatctggc tttgcaggcg gtggatctca acactctgat taaccactca
    26341 ttacagctgg taagccagga tgcaaacagc cgggagatcc agttacgctt taccgccaac
    26401 gacatgttgc cggaaattca ggccgatccg gacaggctga ctcaggtcct gttgaatctc
    26461 tatctcaatg ctattcaggc gattggtcag catggcgtga ttagcgtgac ggccagcgaa
    26521 agcggcgcag gcgtgaaaat tagcgttact gatagcggta agggaattgc ggcggatcag
    26581 cttgaagcta tcttcacccc gtacttcacc accaaagccg acggcaccgg gttggggcta
    26641 gcggtcgtgc ataatattgt tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag
    26701 ggagaaggcg caacgttcac cctctggctt ccggtcaata ttacgcgtaa ggacccacaa
    26761 ggatgacgca cgataatatc gatattctgg tggtagatga tgacattagc cactgcacta
    26821 ttttgcaggc attgctgcgc ggctggggct ataacgtcgc gctggcgaat agtgggcaac
    26881 aggcgctggc gcaggtgcgg gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg
    26941 cagagatgga cggcatcgcc acactaaaag aaatcaaagc gttaaacccg gcaattccgg
    27001 tactgattat gactgcgtac tccagcgttg agacggcagt agaggcgctg aaaaccgggg
    27061 cgctggatta tctcatcaag ccgctggatt tcgataacct gcaggcgacg ctggaaaagg
    27121 cgctcgcaca tatgcacagt attgacgctg aaacgcctgc ggtgtctgcc agccagttcg
    27181 ggatggtcgg taaaagtccg gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc
    27241 catcggaagc cacggtgctg atccacggcg attccggcac cggtaaagag ctggtcgcca
    27301 gggcgattca cgccagtagc gcacggagtg aaaaaccact ggtaacgctc aactgtgcgg
    27361 cactcaacga atccttgctg gaatctgaat tgttcggtca cgaaaaaggg gcgtttaccg
    27421 gagccgacaa acggcgggaa gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg
    27481 atgaaattgg cgatatctcg ccgatgatgc aggttcgtct gctgcgtgcg attcaggagc
    27541 gcgaagttca gcgtgtcggc agcaaccaga ccatctcggt tgatgtccgg ctgattgcgg
    27601 cgactcatcg cgatcttgcc gcagaggtga atgccggacg ttttcgccag gatctctact
    27661 atcgcctgaa cgtggtggcg attgaagttc cgtcgctgcg tcagcggcgg gaagatatcc
    27721 ctctgctggc tggccatttt ctgcagcgct ttgccgagcg taatcgcaag gcggtaaaag
    27781 gttttacgcc ccaggcgatg gatctgctga ttcactacga ctggccggga aatattcgcg
    27841 agctggaaaa cgcggtggaa cgggctgtgg tgctactgac cggggaatat atttccgaac
    27901 gcgagctgcc gctggcgatt gcgagtacgc cgatcccgct ggcgcaaagt caggatattc
    27961 agccgctggt ggaagtcgaa aaagaggtga ttctggcggc actggagaaa acgggcggca
    28021 acaaaaccga agccgcccgt cagttaggga tcacgcgtaa aacgctattg gcaaaactgt
    28081 cgcgttagtt ctgctcgcgt tcgatagcgc gccagccgat atctttccgg cagaagcagt
    28141 cgtcccaatg gatatcggtc attaaggcat aagcgcgttt ctgcgcttct gccacggtat
    28201 gacccagcgc ggtgacgcac agtacgcgcc cgccgctggt cactacctgc tcgtcatccg
    28261 ccagtttcgt gcccgcgtgg aacactttgc cgtcagacac ttcttccagc ggcaggccgt
    28321 ggatcacatc gccggtgcgg taatcgcccg gatatccacc cgcagccatc accacgccga
    28381 gagaagcgcg ttcatcccac tcggacgttt tctcgtccag cttgccttca caggccgcca
    28441 ggcaaagctc aaccagatcg gacttcatgc gcagcataat cggctgggtt tctggatcgc
    28501 caaagcggca gttaaattca ataaccttcg gattgccctg tttgtcgatc atcaggcccg
    28561 cgtagagaaa accggtgtag gtgttgcctt ctgccgccat gcctttcacg gttggccaga
    28621 tgattcgttc catcgtgcgc tgatgaacct catcggtcac tactggcgca ggggagtaag
    28681 cgcccatccc gccggtgttt ggcccggtat ctttattacc tacgcgtttg tgatcctggc
    28741 tggtggccat cggcagcaca tgctcgccgt ccaccatcac gataaagctc gcttcttcgc
    28801 cgtcgaggaa ctcttcgata acaatgcgat gacccgcgtc gccaaaagcg ttgcccgcca
    28861 gcatatcgtg aacagccgct tcggcttctt ccagcgtcat cgcgacgata acgcctttac
    28921 ccgctgccag accgtcagct ttaatgacga tcggcgcgcc cttctcacgc agatacgcca
    28981 gcgcaggttc tacctcggtg aagttctggt attccgccgt agggatctga tggcgggcca
    29041 ggaaatcctt ggtaaacgct ttcgagcctt ccagctgcgc ggcacctgcg gttgggccga
    29101 agattttcag cccggcggcg cggaaggtat ccaccacgcc tttcaccagc ggcgcttccg
    29161 ggccgacgat ggttagatcg atcttttcgt tttgcgcgaa atccagcagc gccgggatat
    29221 cggttacgcc gatagcgacg ttttgcagtg caggctccag cgcagtgcct gcgttacccg
    29281 gagcaacaaa aacagtttca accagcggcg actgggccgc tttccaagcc agcgcgtgct
    29341 cgcgcccgcc gttaccaatc actaatactt tcatctattg ctccattaat ggcggaagtg
    29401 gcgcatgtcg gtgaagagca ttgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc
    29461 atcacggata gaaccgccag gctggattac gcaggtcacg cccgcagcgg cggcggcatc
    29521 aataccgtcg cggaacggga agaacgcgtc agaagccatc gaggaacctt tcacttccag
    29581 accttcatcg gcagctttaa taccggcgat ttttgcggag tacacgcggc tcatctggcc
    29641 cgcgccaatc ccgatggtca tattgttttt ggcatagacg atagcattgg atttcacgaa
    29701 cttcgccact ttccagcaga acagcgcatc acgcagttcc tgttcggtcg gctgacgttt
    29761 ggtgacgacg cgcagttctt ctgcaccgac catccccagg tctcgatcct gaaccagcag
    29821 accgccgttc acgcgtttga aatcaaggcc cggaacacgc tcgccccact gaccgcaggt
    29881 cagaacgcgt acgttctgct tggcggcggt gattttcagg gcttcttcgc tggcggacgg
    29941 cgcaataatc acttcgacga actgacgaga aatgatggcc tgtgcggttt ccgcatccag
    30001 ctcgcggtta aaggcgataa tgccgccgaa tgcggaggtc gggtcggttt tgtacgcgcg
    30061 atcgtaagca tcaagaatgg aattgccgat agccacgccg caagggttgg cgtgcttcac
    30121 aatcacacat gccggctcgg cgaactcttt cacgcactcc agcgccgcat cggtatcggc
    30181 gatgttgtta taagagaggg ctttaccctg aacctgggtt gcggtagcaa cggaggcttc
    30241 tttcacattc tcttctatat agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat
    30301 atcctgcttc ttaatgaagt tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt
    30361 gctttcaccg tggtaagccg gaaccatgct gccgaagtag ttggcaatca tactgtcgta
    30421 ggcggcggta tgttcaaagg ctttgatagc gagatcgaaa cgggttgcaa gcagaagcga
    30481 tccttcgttg gcatccatct ctttaatgat ggcgtcgtag tcgctgctct tcaccacgat
    30541 ggcgacatct ttatggttct tggcggcgga gcgcaccatc gttgggccgc cgatatcgat
    30601 gttctcaacc gcatcttcca gtgagcagcc ttcacgggca acggtctggg cgaacggata
    30661 caggttaaca accaccatat cgataggctg gatctgatgt tcttccataa tggcatcgtc
    30721 ctggccgcga cgacccagaa tgccaccatg tactttcgga tgcagggtct tcacgcgtcc
    30781 atccatcatc tccgggaaac cggtgtaatc ggaaacttcg gttaccggca gacctttttc
    30841 tgctaacaga cgggcagtgc cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc
    30901 ctgggcgaat tcgacgatac cggctttgtc agaaacactg agcagagcgc ggcggactgg
    30961 acgacgttgt tgcatggtaa atcccctgga tttgactatt acagagagcg ttagctgaat
    31021 ttttcgcgaa aaactcagct aacgccccta acggggcatc cttatttttc gcccgcattg
    31081 taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt tcaatggtga tctcaatttt
    31141 gacagtgttt accgtgggaa aaatacagaa attacattga tgattgtgga taactctgtg
    31201 cgtaaaaagg tataaagcgg ggttttgctg gggaatgcag cagtcagtca tttttctgca
    31261 atttttctat tgcggcctgc ggagaactcc ctataatgcg cctccatcga cacggcggat
    31321 gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaatcctga aattcagggt
    31381 tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct gaaagtcgcg
    31441 tcgcaactgc tctttaacaa tttatcagac aatctgtgtg ggcactcgaa gatacggatt
    31501 cttaacgtcg caagacga
//
LOCUS       DAOTRK010000047        31505 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.047, whole genome shotgun sequence.
ACCESSION   DAOTRK010000047 DAOTRK010000000
VERSION     DAOTRK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.047"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(6..1997)
                     /gene="tkt"
                     /locus_tag="QIE41_002051"
     CDS             complement(6..1997)
                     /gene="tkt"
                     /locus_tag="QIE41_002051"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDS3530941.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKEFAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            2275..3033
                     /gene="loiP"
                     /locus_tag="QIE41_002052"
                     /gene_synonym="yggG"
     CDS             2275..3033
                     /gene="loiP"
                     /locus_tag="QIE41_002052"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDS3530942.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYARRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3237..4157)
                     /gene="speB"
                     /locus_tag="QIE41_002053"
     CDS             complement(3237..4157)
                     /gene="speB"
                     /locus_tag="QIE41_002053"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDS3530943.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4293..5024)
                     /locus_tag="QIE41_002054"
     CDS             complement(4293..5024)
                     /locus_tag="QIE41_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530944.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAK
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5170..7146)
                     /gene="speA"
                     /locus_tag="QIE41_002055"
     CDS             complement(5170..7146)
                     /gene="speA"
                     /locus_tag="QIE41_002055"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDS3530945.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7155..7286)
                     /gene="yqgB"
                     /locus_tag="QIE41_002056"
     CDS             complement(7155..7286)
                     /gene="yqgB"
                     /locus_tag="QIE41_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDS3530946.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            7422..7637
                     /locus_tag="QIE41_002057"
     CDS             7422..7637
                     /locus_tag="QIE41_002057"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530947.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            7941..9095
                     /gene="metK"
                     /locus_tag="QIE41_002058"
     CDS             7941..9095
                     /gene="metK"
                     /locus_tag="QIE41_002058"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDS3530948.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            9531..10925
                     /gene="galP"
                     /locus_tag="QIE41_002059"
     CDS             9531..10925
                     /gene="galP"
                     /locus_tag="QIE41_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDS3530949.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11002..11499
                     /locus_tag="QIE41_002060"
     CDS             11002..11499
                     /locus_tag="QIE41_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDS3530950.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            11594..12301
                     /gene="endA"
                     /locus_tag="QIE41_002061"
     CDS             11594..12301
                     /gene="endA"
                     /locus_tag="QIE41_002061"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDS3530951.1"
                     /translation="MYRYLSIAAVVLSTAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            12381..13112
                     /gene="rsmE"
                     /locus_tag="QIE41_002062"
     CDS             12381..13112
                     /gene="rsmE"
                     /locus_tag="QIE41_002062"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDS3530952.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13125..14075
                     /gene="gshB"
                     /locus_tag="QIE41_002063"
     CDS             13125..14075
                     /gene="gshB"
                     /locus_tag="QIE41_002063"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDS3530953.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYSTYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            14184..14747
                     /locus_tag="QIE41_002064"
     CDS             14184..14747
                     /locus_tag="QIE41_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDS3530954.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            14747..15163
                     /gene="ruvX"
                     /locus_tag="QIE41_002065"
     CDS             14747..15163
                     /gene="ruvX"
                     /locus_tag="QIE41_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDS3530955.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(15337..16317)
                     /locus_tag="QIE41_002066"
     CDS             complement(15337..16317)
                     /locus_tag="QIE41_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDS3530956.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDALDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            16335..17039
                     /gene="yggS"
                     /locus_tag="QIE41_002067"
     CDS             16335..17039
                     /gene="yggS"
                     /locus_tag="QIE41_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDS3530957.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17057..17623
                     /gene="yggT"
                     /locus_tag="QIE41_002068"
     CDS             17057..17623
                     /gene="yggT"
                     /locus_tag="QIE41_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDS3530958.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            17620..17910
                     /locus_tag="QIE41_002069"
     CDS             17620..17910
                     /locus_tag="QIE41_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDS3530959.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            17918..18511
                     /locus_tag="QIE41_002070"
     CDS             17918..18511
                     /locus_tag="QIE41_002070"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDS3530960.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            18504..19640
                     /gene="hemW"
                     /locus_tag="QIE41_002071"
     CDS             18504..19640
                     /gene="hemW"
                     /locus_tag="QIE41_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDS3530961.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLNNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGIRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFLDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(19709..20716)
                     /locus_tag="QIE41_002072"
     CDS             complement(19709..20716)
                     /locus_tag="QIE41_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDS3530962.1"
                     /translation="MKKQWIVGTALFMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(20833..21879)
                     /gene="ansB"
                     /locus_tag="QIE41_002073"
     CDS             complement(20833..21879)
                     /gene="ansB"
                     /locus_tag="QIE41_002073"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDS3530963.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGMLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(22055..22774)
                     /locus_tag="QIE41_002074"
     CDS             complement(22055..22774)
                     /locus_tag="QIE41_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDS3530964.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(22958..23284)
                     /locus_tag="QIE41_002075"
     CDS             complement(22958..23284)
                     /locus_tag="QIE41_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDS3530965.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            complement(23284..24003)
                     /gene="trmB"
                     /locus_tag="QIE41_002076"
     CDS             complement(23284..24003)
                     /gene="trmB"
                     /locus_tag="QIE41_002076"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDS3530966.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            24164..25216
                     /gene="mutY"
                     /locus_tag="QIE41_002077"
     CDS             24164..25216
                     /gene="mutY"
                     /locus_tag="QIE41_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDS3530967.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMVRFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            25244..25519
                     /locus_tag="QIE41_002078"
     CDS             25244..25519
                     /locus_tag="QIE41_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDS3530968.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGFTPEDKK"
     gene            25584..26663
                     /gene="mltC"
                     /locus_tag="QIE41_002079"
     CDS             25584..26663
                     /gene="mltC"
                     /locus_tag="QIE41_002079"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDS3530969.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            26865..28121
                     /gene="nupG"
                     /locus_tag="QIE41_002080"
     CDS             26865..28121
                     /gene="nupG"
                     /locus_tag="QIE41_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDS3530970.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGTTDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(28170..30305)
                     /gene="speC"
                     /locus_tag="QIE41_002081"
     CDS             complement(28170..30305)
                     /gene="speC"
                     /locus_tag="QIE41_002081"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDS3530971.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGANWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            30704..31411
                     /locus_tag="QIE41_002082"
     CDS             30704..31411
                     /locus_tag="QIE41_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDS3530972.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
BASE COUNT         7565 a         8338 c         7971 g         7631 t
ORIGIN      
        1 accgattaca gcagcgcttt tgctttcgca acaacgttgt caacggtgaa gccgaactct
       61 tcaaacagct gctctgccgg agcagattca ccgaaggtgg tcatgccgac gatagcaccg
      121 ttcaggccaa catacttgta ccagtagtca gcaatgccag cttccaccgc aacacgagcg
      181 gtaaccgctt tcggcagtac ggattcacgg taagccgcgt cctgcttgtc gaatgcgtcg
      241 gtagacggca tggaaaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggcg
      301 gcaacagcca gttcaacttc agaaccggtc gcgatgaaga tcagttccgg ctgaccagca
      361 cagtctttca gcacataacc accgcgcgcg atgtttgcca gctgctcttc agtacgttcc
      421 tgctgcgcca ggttctgacg ggagaggata agcgcagtcg ggccgtcctg acgctcaacg
      481 ccgtatttcc acgctactgc ggattcaacc tggtcacacg gacgccatgt agacatgttc
      541 ggggtcacgc gcagagaagc gatctgttct accggctggt gagtcgggcc atcttcgccc
      601 agaccgatgg agtcgtgggt gtaaaccatc acctgacgct gtttcatcag cgcagccata
      661 cgtacggcat tacgtgcgta ttccacgaac atcaggaagg ttgaggtata cggcaggaaa
      721 ccaccgtgca gggagatacc gttggcaatc gcagtcatac cgaattcacg tacaccgtag
      781 tggatgtagt tacccgcagc atcttcgttg attgctttag aaccagacca cagggtcagg
      841 ttagacggcg ccaggtctgc agaaccgccg aggaattccg gcaacagcgg cccgaacgct
      901 tcgatagcat tctgagacgc tttacggctg gcgatcttcg ccggattagc ctgaagttta
      961 gcgatgaact ctttcgcttt ggcgtcgaag tcagacggca tttcgccttt catacggcgg
     1021 gtaaattcag cagcttcctg cggataagct ttcgcgtaag cagcgaattt ctcgttccat
     1081 gcgaattctt tcgcctggcc tgcttctttc gcatcccact gagcatagat ttcagacggg
     1141 atttcgaacg gcgcgtattt ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg
     1201 cccagcggcg caccgtggga gtcgtgggta ccggctttgt tcggggaacc gaaaccgatg
     1261 atggttttgc acatcagcag ggacggtttg tcagtcacgg cgcgcgcttc ttctactgcg
     1321 cgtttgatgg atgccgcgtc atgaccgtcg atatcgcgaa taacgtgcca gccgtaagct
     1381 tcgaaacgca ttgcggtgtc gtcggtaaac cagccttcaa cgtgaccatc gatagaaata
     1441 ccgttgtcat cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagagcaa
     1501 acttcgtggg agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg
     1561 tcgacgatgt cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc
     1621 ataccgactg cgttggcaat accctgaccc agcggaccgg tggtggtttc tacacctgcg
     1681 gtgtaaccca cttccgggtg acccggagtt ttagagtgca gctgacggaa gtttttcagt
     1741 tcttccatcg gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg
     1801 tggccgttgg acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt
     1861 ttcaggaaat cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg
     1921 tgaccggatt tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc
     1981 tctttacgtg aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact
     2041 tgacgacagc gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgct
     2101 cgtcatgtac atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt
     2161 tgcgcaatcc actcgcccgt ccgctgcgct tttccttata ctgagactga gcgtcgattc
     2221 acctgcaaac ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa
     2281 attcgcgcct tattggtagc aatgagcgtg gctacggtgc tgaccggttg ccagaatatg
     2341 gactccagtg gactgctctc atcaggagcg gaagcttttc aggcttacag tttgagtgat
     2401 gcgcaggtga aaaaactgag cgatcaggca tgccaggaga tggacagcaa agcgacgatt
     2461 gcgccagcca atagcgaata tgctagacgt ctgacgacaa tttctcgcgc gttaggcgat
     2521 aacatcaacg gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt
     2581 gcaatggcta acggctgtat ccgcgtctat agcgggctga tggatatgat gacggataac
     2641 gaagtcgaag cggtgctcgg tcacgaaatg gggcacgtgg cattaggcca tgtgaagaaa
     2701 ggaatgcagg tagcacttgg tacaaacgca attcgcgttg cggcggcctc tgcaggcgga
     2761 attgtcggca gtttgtcaca atcacaactg ggagacctgg gcgaaaagct agtcaattcg
     2821 caatttaccc aacgtcagga atcagaggcc gatgattact cctacgattt gctgcgccaa
     2881 cgcggtatca gcccggcagg tcttgccacc agctttgaaa aactggcgaa actggaagaa
     2941 ggccgccaaa gctcaatgtt tgacgaccat cctgcatccg ccgaacgcgc ccagcatatt
     3001 cgcgatcgta tgagcgcgga tggagttaag taaagccagg tagtgccgaa cgtaggtcgg
     3061 ataaggcgtt cacgccgcat ctgacaagaa tggcacattt gttactccgt gcgcattgcc
     3121 ggatgcgatg ctggcgtatc ttatccggcc tacgggtgcc gagtgtaggt cggataaggc
     3181 gttcacgccg catccgacat taatggcaca tcacccgcgc gcctcttatc tggcgcttac
     3241 tcgccctttt tcgccgcctg gatatacagc atctccagcg ccagcgtcgc cgcagccaga
     3301 gcggtgattt ccgactgatc gtatgccgga gccacctcca ctacgtccat cccaacgatg
     3361 ttgagatctt tcaagccgcg taccagttta atggcgcgat cggaggtcag gccgccaatt
     3421 accggcgtac cggtgcctgg tgcaaaagca ggatccaggc agtcgatatc aaaggtcagg
     3481 taaaccggca tatcacccac aatctgtttc acctgggcga taatgtcatc cacgctgcga
     3541 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     3601 ataccaatct gcacagaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     3661 atggtgccgt ggtcaaattc acaaccgtta gcgtaggtat cggtgtgggc atcaaagtgt
     3721 accaacgcca ttttgccgaa atgcttcgca tgagcgcgca gcagcggcag cgtaacaaag
     3781 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     3841 tgcagcttct cgctcatctc acgggcatct ccgaaggcat ataccagatc gccgcagtcc
     3901 acgacgttca ggcgctcacg catgtcgaaa ttccacggga agcgattgtg ttcccaggcc
     3961 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     4021 gtggccatat cgaacggcac gccagtaatc acccagtcag catcgctgtc atatggctgg
     4081 aagttcatcg gcaggcgtaa aaaaccaaag gcgttggaaa ccagtgagtt atcgtattga
     4141 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     4201 cgtcgttagg cccgacgagg aagggttgga tttgtgaaac acaagtttgt gctaattatc
     4261 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     4321 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt tttcgccggg
     4381 ccttgtaatt caaaggcggc aaagccgatt acaccggtat tacgcacatc gccttgcgcc
     4441 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc ggtatgttta
     4501 tctttgcgga atcctttaat cgctaacgaa tctccggcat tcacaatata accgttgtta
     4561 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat caaccgtagc aacgatctcg
     4621 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc gactctggca
     4681 tggacaatat agcgtggttc attattggat gaatcataaa cccgcgtaat aggtagagaa
     4741 ttaaaattac catcgcgaat agcatattcc agttcgttaa tacgaatgct ataaacgcgg
     4801 tcgtagccgg aaggaacatt agtcgaataa ctaataacga tcatctctgc cggatcgcgc
     4861 caggctcgtt cagcagatac gccctgaacc gttgagtgca catccccacc ccaggcggtg
     4921 ccgacattgc ctgatgcact gctattatac tgcgcattac tggaacaacc agccagcagc
     4981 acgattaatg ccaccaacgc gctacgtact ttccattttt tcataaccgt tccctctgca
     5041 tttaattaat gcagcgattg tagggagatt atttaataag aaaagcagct aaattatttt
     5101 taagaatata taaaaagaaa gcgggccgat ttacgtaaat aaatcgcccc gccagcagaa
     5161 taatcttaat tattcatctt caagataggt ataaccgtac aaacctgcct cgaactcttc
     5221 caggaactgt tgttgcagtt cagcatcaag atcggttttc ttcacctgat cgcggaactg
     5281 agttaacagc gttttcggat cgagctgcac atattgcagc atgtccgcca cggtatcgcc
     5341 ttcgtcagac agttctacct ctacgctacc atcagggaag acgaacacgt caaccgcttc
     5401 ggtatcaccg aacaggttgt gcatgttgcc gaggatctcc tgatatgcgc cgaccataaa
     5461 gaaaccgagc atcggcggat tctccggatc gtactccggc ataggcattg tcgtggcaat
     5521 accgtcacca tcaatatagt ggtcgatagc accgtctgag tcacaggtaa tatccagcag
     5581 cacagcgcgg cgttccggca cttgatccag cccttccagc ggcagaaccg ggaacaactg
     5641 gtcgatcccc cacgcatccg gcatcgactg gaacagcgag aagttgacgt acattttgtc
     5701 cgccatacgt tcctgcagct cgtcgataat cggacgatga gcacggtttt gcggatccag
     5761 ctgcttttgc acttcatggc acatgctcaa ataaagctgc tcagcccatg cgcgttcttg
     5821 caggctaaag gtgccggaag agtagccgat atgaatgtcg tgcagatcca tctggctgtc
     5881 gtgtaaccat tcacgcagag aacggcgagt tcccggttcg tgcatttcct gccaggtttc
     5941 ccacatgctt tgcagggcgc gcggtgcatc ttctgcaggc gcggtcggca ccgtgtattc
     6001 gttacgttcc acgccgatga tattcgacac cagcacggtg tgatgcgcag tcaccgcacg
     6061 acccgattcg gtgattaccg tcggatgtgg cagaccattt tcttcacacg catcaccaat
     6121 cgcccagatg atgttgttgg cgtattcatt gaggccgtag tttacggagc agtcagactg
     6181 agaacgggtg ccttcatagt ccacgcccag accgccgccg acgtcgaagc actgaatatt
     6241 gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat tcacgaacgc ctgtcgcgat
     6301 atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc agcagttgca ggctgtcgag
     6361 acgcccggct tcacgcagcg tttcaaccag ttgcagtact tgagtcgccg ccaggccaaa
     6421 cttcgatttt tccccgccgg aggactgcca tttacccgag ccctgcgaag ccagacgtgc
     6481 acgcacgccc agacgaggaa cgacattcag acgttctgct tcatccagca caatggcaat
     6541 ttctgacatc ttctcaatga ccagatagac cttgtggccc atcttctcgc caattaatgc
     6601 caggcggata tattcgcggt ccttataacc gttgcagacg atgacgctac gggtcatgcc
     6661 agcatgtgcc agcaccgcca tcaactcggc tttggaaccg gcttccaggc ccagcggttc
     6721 gcccgaatga atcagggact caatcacgcg gcggtgctgg ttaactttga tcggataaac
     6781 gaggaagtaa tcgccgttat agccgtagga ttcccgcgct cgtttgaacg cggcgttaat
     6841 ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac agtgcaggca gacgctgacc
     6901 ctgtgcttcg cgagttttca ctaactgcgc gagatcgacg cgagcttccg ggacgtccgg
     6961 gtccgggcac acgctgatgt ggcccagctc gttaacgtca tagtagttat tgccccacca
     7021 ggcaatattg taagtacgca gcatcttgct ggcttcctgg gagctcattg caacctcctg
     7081 catggagcgt agtacaccgt gttcgcccgc tgacgaaggc aaacccatag acatgtcgtc
     7141 agacatagcg aacctcaaat tattttatta agtgtaaaac agttaacgac tatcgcagcc
     7201 tggaactgcg ataacaaccc ataagcacat ggattttcca gcagtgaatg ctgacgctcc
     7261 aactgcgcaa ccggtttctt tttcataaca ttattaagca cataaccgaa cgtaagtgtg
     7321 acagttcggc gaaaccacga gaaaactctt gtttttacaa gagcgccctt gttcagtcct
     7381 cagtagctgt aaccagctct tgaatcctga gaagcgccga gatgggtata acatcggcag
     7441 gtatgcaaag cagagatgca gagtgcgggg aacgaatctt caccagaacg gtgagacagg
     7501 ttaagcagca gacaacggtt cattatttcg tatcacctcc acggccgcct gttaagacga
     7561 acccacaagc caaaactctg ttttcaaccc ggctggaagt ggcaacacga aagaaacgtc
     7621 gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc cacaatggca gaaaattgca
     7681 aaagataaat acgcagaatg ccagcattgt caggaaaaat ttccagccac gtttttaaca
     7741 gaatgagaca cgattcaaaa aaaagtggaa attgggtgaa gaactgacct aaaatagcca
     7801 tccagatgtt aatccatcca taccgattaa cactcagact gccagtgttt ttaacctgca
     7861 gagtcgtggt aggatccgct accacagaaa atccacacaa cagtttgagc taaccaaatt
     7921 ctctttaggt gatattaaat atggcaaaac acctttttac gtccgagtcc gtctctgaag
     7981 ggcatcctga caaaattgct gaccaaatct ctgatgccgt tttagacgcg atcctcgaac
     8041 aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa aaccggcatg gttttagttg
     8101 gcggcgaaat caccaccagc gcctgggtag acatcgaaga gatcacccgt aataccgttc
     8161 gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc taactcctgt gcggttctga
     8221 gcgccatcgg taaacagtct cctgacatca accagggcgt tgaccgtgcc gatccgctgg
     8281 aacagggcgc gggcgaccag gggctgatgt ttggctacgc aactaatgaa accgacgtgc
     8341 tgatgccagc acctatcact tatgcacacc gtctggtaca gcgtcaggct gaagtgcgta
     8401 aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag ccaggtgact ttccagtatg
     8461 acgacggcaa aatcgttggc attgatgctg tcgtgctttc cactcagcac tctgaagaga
     8521 tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat catcaagccg attctgcccg
     8581 ctgaatggct gacttctgcc accaaattct tcatcaaccc gaccggtcgt ttcgttatcg
     8641 gtggcccgat gggtgactgc ggtctgactg gtcgtaaaat catcgttgat acctacggcg
     8701 gcatggcgcg tcatggtggc ggtgcgttct ccggtaaaga tccatcaaaa gtggaccgtt
     8761 ccgcagccta cgcagcacgt tatgtcgcga aaaacattgt tgctgctggc ctggccgatc
     8821 gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga accgacttcc atcatggtag
     8881 aaactttcgg tactgagaaa gtgccttctg aacaactgac tctgctggta cgtgaattct
     8941 tcgacctgcg cccatacggt ctgattcaga tgctggatct gctgcacccg atctacaaag
     9001 aaaccgcagc atacggtcac tttggtcgtg aacatttccc gtgggaaaaa accgacaaag
     9061 cgcagctgct gcgtgatgct gccggtctga agtaatcttt cttcacctgc tttcaaaggc
     9121 cagcctcgcg ctggcctttt tcttttacct caccgcagtg gacaggcgtt cacgccgcat
     9181 ccggcaaaaa accgcccgca caataacatc attcttcctc aacacgtttt accgcaaatt
     9241 atcatcacta ctgaaatcga ttacaccaac cacaccagcc aaatatttgt aatattttca
     9301 tattattatt cagctttcac agttgttaca tttcttttca gtaaagtctt aattgcagat
     9361 aacaaccttt aatctatgat gatataactc aattattttc atgcacttaa atcataacta
     9421 agataaatgt tagtgtaagc gattacactg atgtgatttg cttcacatct ttttacgtcg
     9481 tactcaccta tcttaattca caataaaaaa taaccatatt ggagggcatc atgcctgacg
     9541 ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc cttgccgctc
     9601 tggcgggatt actctttggc ctggatatcg gtgtaattgc tggcgcactg ccgtttattg
     9661 cagatgaatt ccagattact tcgcacacgc aagaatgggt cgtaagctcc atgatgttcg
     9721 gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt taaactcggg cgcaaaaaga
     9781 gcctgatgat cggcgcaatc ctgtttgttg ccggttcact gttctctgcg gctgcgccaa
     9841 acgttgaagt actgattctt tcccgcgttc tgctggggct ggcggtgggt gtggcctctt
     9901 ataccgcacc actgtacctg tcagaaattg cgccggaaaa aattcgcggc agtatgatct
     9961 cgatgtatca gttgatgatc actatcggga tcctcggcgc ttatctttct gataccgcct
    10021 tcagctacac cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg gcaattttgc
    10081 tgctgattgg tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc aaacgccgtt
    10141 ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa gcgaaacgcg
    10201 aactggatga aatccgtgaa agtttgcagg ttaaacagag tggctgggcg ctgtttaaag
    10261 agaatagcaa cttccgccgc gcggtgttcc ttggcgtact attgcaggta atgcagcaat
    10321 tcaccgggat gaacgtcatc atgtattacg cgccgaaaat cttcgaactg gcgggttata
    10381 ccaacaccac cgagcaaatg tgggggacag tgattgtcgg cctgaccaac gtacttgcca
    10441 cctttatcgc aatcggcctt gttgaccgct ggggacgtaa accaacgcta acgctgggct
    10501 tcctggtgat ggctgctggt atgggcgtac tcggtacaat gatgcatatc ggtatccact
    10561 ctccgtcggc gcagtatttc gccatcgcca tgctgctgat gtttattgtc ggttttgcca
    10621 tgagtgccgg tccgctgatt tgggtactgt gctccgaaat tcagccgctg aaaggccgcg
    10681 attttggcat cacctgctcc actgccacca actggattgc caacatgatc gttggcgcaa
    10741 cgttcctgac catgctcaac acgctgggta acgccaacac cttctgggtg tatgctgctc
    10801 tgaacgtact gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa cacgtttcgc
    10861 tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaatc ggcgctcacg
    10921 attaatctcc tcaggcttcc tcccatcatg gaggaagcca cctcttgcag tcatcttttc
    10981 ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc ctatcgccat ccaacaggcc
    11041 gttatgcgtc gcctgcggga aaaactcgcc caggccaacc tgaagctagg gcgtaactac
    11101 ccggagccaa aactctctta cacccagcgc ggaacctctg ccggaacagc ctggctggaa
    11161 agctatgaaa ttcgtctcaa tcccgttttg ctgttggaaa acagtgaagc ctttattgaa
    11221 gaagtggtgc cgcacgaact ggcacatttg ctggtatgga aacatttcgg ccgcgtagcg
    11281 ccacatggca aagagtggaa gtggatgatg gaaagcgtgc tgggcgttcc cgcccgtcgt
    11341 acgcatcagt tcgaactgca atccgtgcgc cgcaacacct tcccctaccg ctgcaagtgc
    11401 caggagcatc agcttaccgt acgccgtcat aatcgcgtag ttcgtggcga agccgtctat
    11461 cgctgtgttc actgcggcga acagctggtt gcgaaataac cacctgaact atcaggaact
    11521 ttcctgatct ggctgattgc ataccaaaac aactttcgct acgttgctgg ctcgttttaa
    11581 cacggagtaa gtgatgtacc gttatttgtc tattgctgcg gtggtactga gcacagcatt
    11641 ttccggcccg gcattggccg aaggtatcaa tagtttttct caagcgaaag ccgcggcggt
    11701 aaaagtccac gctgacgcgc ccggtacgtt ttattgcgga tgtaaaatta actggcaggg
    11761 caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag gtgcgaaaaa atgaaaaccg
    11821 tgccagccgc gtagagtggg aacacgtcgt tcctgcctgg caattcggtc accagcgcca
    11881 gtgctggcag gacggtggac gtaaaaattg cgccaaagat ccggtctatc gcaagatgga
    11941 aagcgatatg cataacctgc agccgtcagt cggtgaggtg aatggcgatc gcggcaactt
    12001 tatgtacagc cagtggaatg gcggtgaagg ccagtacggt caatgcgcca tgaaggtcga
    12061 tttcaaagaa aaagttgccg aaccaccagc gcgtgcacgc ggtgccattg cgcgcaccta
    12121 cttctatatg cgtgaccaat acaacctgac actctctcgt cagcaaacgc agctgttcaa
    12181 cgcatgggac aagatgtatc cagttaccga ctgggagtgc gagcgtgatg aacgcatcgc
    12241 gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct tgccaggcgc gaaagagcta
    12301 acctacacta gcgggattct ttttgttaac ccctacccca cgcgtacaac cgcgtgggga
    12361 gacgacgcgg atttttaact atgcgtatcc cccgcattta tcatcctgaa ccactgacca
    12421 gccattctca catcgcgctt tgcgaagatg ccgccaacca tatcgggcgc gtactgcgta
    12481 tggggccggg acaggcgctg caattgtttg acggcagcaa ccaggtcttt gacgccgaaa
    12541 ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct ggaaggccag atcgacgatc
    12601 gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc gcgtggtgaa aaaatggaat
    12661 ttactatcca gaaatcgatc gaactcggtg taagcctcat tacgccactt ttttctgagc
    12721 gctgcggcgt taaactggat agtgaacgtc tgaacaagaa gctccagcag tggcagaaga
    12781 ttgcaattgc tgcctgtgag cagtgtggcc gtaaccgggt gccggaaatc cgtccagcga
    12841 tggatctgga agcctggtgt gcagagcagg atgaaggact gaaactgaat cttcacccgc
    12901 gcgccagtaa cagcatcaat acgttgccgt taccggtcga acgcgtccgc ctgctgattg
    12961 gcccggaagg cggtttatcg gcagatgaaa ttgccatgac tgcccgctat caatttactg
    13021 atatcctgtt gggacctcgc gttttgcgta ctgagacaac tgcgctcacc gccattaccg
    13081 cgctacaagt gcgttttggc gatttgggct aacggagaag aataatgatc aagctcggca
    13141 tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga ttccagtttc gctatgttgc
    13201 tggaagcaca gcgtcgtggt tacgaacttc actatatgga gatggccgat ctctatttga
    13261 tcaatggtga ggctcgcgct cgtacccgta ctttgagcgt cgaacagaac tacgataaat
    13321 ggtacgagtt cacgggtgag caggatctgc cgctggccga tctcgatgtg atcctgatgc
    13381 gtaaagaccc gccgtttgat accgagttta tctactcgac ctatattctg gaacgtgccg
    13441 aagataaagg gacgctgatc gttaacaagc cgcagagcct gcgcgattgt aacgaaaaac
    13501 tgttcactgc ctggttctct gacttaacac cagaaacgct ggtcacacgc aataaagcgc
    13561 agctaaaagc tttctgggaa aaacacagcg acatcattct taagccgctg gacggtatgg
    13621 gcggcgcgtc gattttccgc gtgaaagaag gtgatccaaa cctcggcgtg attgccgaaa
    13681 ccctgactga gcatggcact cgctactgca tggcgcaaaa ttacctgcca gccattaaag
    13741 atggcgacaa acgcgtgctg gtagtggatg gcgagccggt gccgtattgc ctggcgcgta
    13801 ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg tggtcgcggt gaacctcgtc
    13861 cgctgacgga aagtgactgg aaaatcgccc gtcagattgg gccgacgctg aaagaaaaag
    13921 ggctgatttt tgttggtctg gatatcatcg gcgaccgtct gactgaaatt aacgtcacca
    13981 gcccaacctg tattcgtgag attgaagcag agtttccggt gtcgattacc ggaatgttaa
    14041 tggatgccat cgaagcacgt ttacagcagc agtaacccac cttagcgaga aggatctcgt
    14101 tgagactctg agtgacagcg cccttctttc cacgcatact gggcgctgtt gcttttttga
    14161 accaggaaac agaacctctg acaatgaatt tacagcatca ctttcttatt gccatgcctg
    14221 ctctccagga tccgattttc cgtcgttccg tggtctacat ttgcgaacat aataccaatg
    14281 gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct caaaattgaa gggattctgg
    14341 aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat ccgtctggat aaaccggtta
    14401 tgctcggcgg tccgctggct gaagatcgcg ggtttatttt gcatactccg ccttccaatt
    14461 ttgcttccag cattcgcatt tcagacaaca cagtgatgac cacttcccgc gatgtgctgg
    14521 aaacgctcgg caccgataaa caaccgtctg acgtattggt ggctctgggt tatgcctcct
    14581 gggagaaagg tcaactggaa caagaaattc tcgataacgc gtggctaacg gccccggcag
    14641 atctgaatat tctgttcaaa acgccgattg ccgaccgctg gcgcgaggcg gcaaaactga
    14701 ttggtgtgga tattctcacc atgcctggtg tggcaggaca cgcctgatga gtggaacctt
    14761 actcgccttc gacttcggca ccaaaagcat tggcgtagcg gtcggccaac gcattaccgg
    14821 caccgctcgc cctttgcctg caattaaagc gcaggacggt acgccggact ggaacattat
    14881 cgagcgttta ctgaaagagt ggcagccgga cgaaatcatc gtcggtttgc cgctgaatat
    14941 ggacggcacc gaacagccgt taaccgccag agcgcgtaaa tttgccaacc gtattcatgg
    15001 tcgtttcggt gttgaagtaa agctccatga cgagcgtctt agcaccgtgg aggcacgttc
    15061 cggtctgttt gaacagggcg gctatcgggc gctcaacaaa ggcaaagtcg actctgcctc
    15121 tgcggttatt attctcgaaa gctatttcga gcagggatat taaggcgatt tactagcgca
    15181 cagatttctg acaatgtgct cgttgtttca tgttggatgc ggcgtaaacg ccttatccaa
    15241 cctacagtga gtgcgaattc aaagttacgc ttcccttgta ggcccgataa gcttgcgcat
    15301 cgggcatatt tacaattcgt atcagacaca gcaacgtcac aagcgccctt ctttcacccg
    15361 ctgctgataa ctctgctgaa acgttatcat ccccacctgc tgcccggttt gaataacatg
    15421 cggtaactgg tgggttttcc cttcacgaat caaattcccc accgcgggtg tgttaatcag
    15481 taattcaaac agcgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac
    15541 cgcccgtaaa ctacctgcca gttgattacg cacgggatct ttttcctgcg ccggaaacga
    15601 atccaccagt cgctcaactg cctgcgctgc gccgcgcgtg tgtaatgtcg ccagcaccag
    15661 atgcccggtt tctgccgccg tcagcgccag acggattgtc tcgctatcac gcagctctcc
    15721 gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc aatcccgatg cgaaagtcat
    15781 acagtgcaaa ccaatctccc gctgttggat caaacatcgc tgactggtat agagatattc
    15841 cacaggatct tccagcgtca gaatatgcgc atcggcatgt tgattgagat agccaaccat
    15901 cgccgccagc gtggtagatt tgccgctccc cgtcgccccc gtcaccagaa tcaggccatt
    15961 ctcgctcttg agtaattccg gcaataccgg tggcgcacca agctgttcga gctgtggaca
    16021 gtgcgaaggt aacaaccgta atgccagaga aataccttgc cgttgcgcga acgcactgcc
    16081 acgcaaccgc tggttttccg ccagcgacac agcaaaatcc aactgaccat tctccagcaa
    16141 tattgtccgc tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccagcgcatc
    16201 aaacggcgca gcttccattc gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag
    16261 gtgtagatcc gagacgttat gctttacact aagggccaca atttcttcca tattcatact
    16321 aagatcctcg gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct
    16381 cagcggctgc aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta
    16441 aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg
    16501 aaaactacgt tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag
    16561 gattagaatg gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc
    16621 atttcgactg gtgtcatacc atcgaccgtt tgcgcatcgc tacccgtctg aacgaccagc
    16681 gcccggcaga acttccccct cttaacgtac tgattcaaat taacattagt gatgaaaaca
    16741 gtaagtccgg gattcaactg gctgaactgg acgagctggc agctgcggtc gctgaactac
    16801 cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc
    16861 agtttgaagt tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc
    16921 atatcgacac gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta
    16981 gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag
    17041 gaattaaagg aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta
    17101 taccatggtg ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc
    17161 cttctcacag tttgtagtga aggtaacgca gccgattatc gggccactgc gccgcgttat
    17221 tccggcaatg ggccccattg atagcgcctc gctgctggtt gcctatattc tcagtttcat
    17281 taaagccatc gtgctgttta aagtggtgac cttcctgcca atcatttgga ttgccggttt
    17341 attgattctg ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat
    17401 tatgagctgg gtaagccagg gtcgtagccc gattgaatac gtgctgattc agctggccga
    17461 tccgctgctg cgcccgattc gccgcttgct accggcaatg ggtgggattg atttctcgcc
    17521 gatgatcctc gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tcttgcaggc
    17581 gaccggaaat atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat
    17641 gatgacggtc tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc
    17701 ggtttacatg gcgacgaggt aaaagtcgcc attaccgcgc cgccggttga cggccaggcc
    17761 aacagccatc tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg
    17821 attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc
    17881 ccgccagaaa tcgcggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac
    17941 cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat
    18001 cgtggcccaa acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga
    18061 aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc ggtttaccgg caattgccga
    18121 cgactccggt ctggcggtag atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta
    18181 ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa ctgctggaaa caatgaaaga
    18241 cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc
    18301 ggaagatccc actccgctgg tgtgccacgg cagctggccg ggcgtgatta ctcgtgaacc
    18361 agcgggcact ggtggctttg gttatgatcc aatcttcttc gtaccttccg aagggaaaac
    18421 tgctgccgaa ctgacccgcg aagaaaagag cgccatttcc caccgtggtc aggcgttgaa
    18481 actgctgctg gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca
    18541 tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag
    18601 aagtgccgca cgacgattat gttcagcatc tgcttaacga tctgaacaac gatgtggctt
    18661 acgctcaggg ccgtgaagta aagacaatct ttattggcgg tggtacgccg agcctgcttt
    18721 ccggcccggc gatgcaaacg ctgctggacg gcatacgtgc gcgtttgccg ctggcagcgg
    18781 atgcagaaat tacgatggaa gcgaatcctg gtacggttga agccgatcgc tttgttgatt
    18841 atcagcgtgc tggtgtgaac cgcatctcca ttggcgtaca gagttttagc gaagaaaagc
    18901 tgaaacgact tgggcgcatt catggcccgc aagaagcgaa acgagcggca aatctggcga
    18961 gcggtttagg gttacgtagc tttaacctcg atttgatgca tgggctaccg gatcaatcac
    19021 tggaagaggc gcttggcgat ctacgccagg ctattgaact gaatccgccg catctttcct
    19081 ggtatcaact gaccatcgaa cccaatacgc tgtttggctc gcgccctcct gtactgccgg
    19141 acgatgacgc gctgtgggac attttcgaac aggggcatca gttattaacc gcagcgggtt
    19201 atcagcaata tgaaacctcc gcttacgcca aacccggtta tcagtgccag cacaatctca
    19261 actactggcg ctttggtgac tacatcggta ttggctgcgg cgcgcatggc aaagtgacct
    19321 tcctggatgg gcgcattctg cgtaccacca aaacgcgtca tccgcgtggt tttatgcagg
    19381 ggcggtatct ggaaagccag cgtgatgtcg aagccgcaga taagccgttt gagttcttta
    19441 tgaatcgctt ccgtttgctg gaagccgcgc cgcgcgtgga gtttagccag tatactggcc
    19501 tttcagaaga ggttattcgc cctcagttag acgaggctat tgctcagggt tatctcacag
    19561 aatgtgcgga ttactggcag ataacggaac atgggaagtt gtttttaaat tcgctgctgg
    19621 agctttttct ggctgagtaa atttgtattg ccggatgcgg cgtgcgtgta cgcagcatcc
    19681 ggcaacaact catgcgtcat cgcaaaaatt acttaatcac cccattgcgc cgcagttgtt
    19741 cggtatcttt tttccagcgt tcattagcct ggttgatatc gtcaattttc atcaaaatcg
    19801 cgactttttt actgtttatt tctgaaactt tcatcgttaa cggatattgc cctttttcga
    19861 gcgttatcca gtcatcctgc aatttacggc taagtttttg tgattgcaga aaagtctgtc
    19921 gccgttgttc gttgatatca aaatctcgtt tctgctgaat tggcacttca taacactctt
    19981 cactctgttt atgacatgcc gcaatcgccg gttgataaac ctctttgtca tatttaaccg
    20041 cgaacgcctc actatcgttc tgtttattaa aatcatcacg ctgttggtga atttttttaa
    20101 aggcgtcttc gcgggtcatt tgcttaccgt ttgcatcttt tccccaatat gcatccgctg
    20161 ctttctgcgc cgccaccatt tgcttatcaa gcgcttttac atcctttttc agtgcaggaa
    20221 tcgagttatt ctggttattc aacgcagact caagctgact aaagcgataa ttgaaatctt
    20281 cgctattcac gatcagatat ttattattcg ttttgatatc gtcaaccacc cgcccacgat
    20341 cgctggctgc cgcctgaaaa gagtaaaagt tcaccgacca gccagacgcg ggcgttcctt
    20401 tactggttaa catcgccgag aatttcaccg gtttatcttt cgtccaggtc tgttcgagca
    20461 gctcgtaatc tgccaactga ccgacccagg tatagagatc gtcactggaa gagacatcgc
    20521 cttccgctga ccaggtggcc tgattaccct tagcatcaag attttttaag gtgatgacat
    20581 caagcttgag aatgccgtga tactgctttt tgaattgatc ttttaagata ttttcagttg
    20641 gcggttcacc atctgcccag acattaccag tcatcaacat aaacagcgcc gtaccaacaa
    20701 tccattgctt tttcacccga aaaccctcgc gcggaataat ttattccggt gattatcatt
    20761 agggtaataa aaattggcaa tggacgtgag tctgaagtga ataagccccg gcgcgatacc
    20821 ggggcgaagt gattagtact gattgaagat ctgctggatc tgctgcggat ctttagtttg
    20881 cgtcagagcc agttgcagca gaacgcgcgc tttttgcggg ttcaacatgc cggaggcgac
    20941 gaagccgtat ttcgcatcat ccacttcggc atcctgagtg gtagcgcccg tcggtacgcg
    21001 ggaagaacgc actactgcag tgccgttttt cgcagcggtt gcaagggtgt caaatacggt
    21061 tttatacagg ttgccgttac ccacgccagc gctaacgatg ccatcatagc ccgcatctac
    21121 cagtgcttta gccggaagat cggatgcgtt agcgtagtta taaacaatgc cgactttcgg
    21181 cagttcattc agcttagaga catcgaacgg cgtgtcgctg gtgtgcttac gtgccggggt
    21241 acgttggtag tcaatcttac cgttgtgaat gtaacccaga ggaccgtagt taacagactt
    21301 gaaggtcgct acatcggtgg tgttggtttt ggtgacatca cggccatcaa gcacggtgtc
    21361 gttcatcact accagtacgc cacgattagc ggaggcttta tcagctgcgg tcactaccgc
    21421 gttatacagg ttgaatggac cgtctgcgct catagacgtg gacgggcgca ttgcaccgac
    21481 catcaccacc ggtttgtcgc atttcaccgt caggtcgagg aagtaagcgg tttcttccat
    21541 cgtgtcggta ccgtgggtaa tgacgaagcc gtcagtttta tcgcagtcgg tgttaatttt
    21601 tttcgccagt gtcagccaga catcatcgtt catgtcctgg gagccaatat tcactacctg
    21661 ctcgccttta acgttcgcaa tgtccttcag ttgcggcacc gcattaacca gattttctac
    21721 gccaacttta cccgctgtgt agttagattt ggttgcggag tcaccaccac cggcaatggt
    21781 cccgccggtt gctaaaatgg tgatattggg taatgccaat gctgcaccac taaaacccat
    21841 aaccagtgcg gcaagtgccg tctttttgaa aaactccatt tcattcctcc agttacgtga
    21901 acgctacgca ttatccctta gctttgcatg ggaaatttga cgttaaacaa tttacaacgt
    21961 gaatatattt tggagatcta caaagttaga ggcaggtaac aaaacgaaga attaaacggc
    22021 ataaaaaagt attatgccgt cttaaaatag aggattattt taaattcccg accagggctt
    22081 tgcggctatc ttccagagtc acaacgcggc tacaaacatc tttgccaaac tgctggaaat
    22141 ctttttcctg ctttttccac tcggtttgga ttgaggattg caggccgccc aggcttccca
    22201 gcacgttctg taatgggtta ccgccgcttt tcagcaccgc tttcgcgccc atttcattaa
    22261 tgctgtcctg taaaattccg cccattgcct gattcactaa ttgctggcct tcggcacgaa
    22321 cctgatcaat ggctttatag tgaaacgtca ggccatcgct gcgcgtttcg ataatgcggt
    22381 tcatctgctc tttcagctgc gcatcaagtt tggtcagacg gctgcgcatt ttgctgcttt
    22441 cgcccatctc ctgaacgata attttatcca gcgcaatacg agctttctcg acgcggcttt
    22501 tcgcgccttc atcaatccac ggcagggtgc tacgtagttc agcctgataa tccttcgcct
    22561 gctcgcgctg ggcggcattc agggaatatt gcttaccgtt atacatcacg ttgccgtctg
    22621 gcgtgatcac cagattgccg ttttcgccct tcacctgcac ggtttgcggg ctgacaatca
    22681 catcgtcacg cggcgtgacg ctgcactggt agtcggcgtg agcggtcatt gccgtcactg
    22741 aaagtgctgc cgccagcagc attttgcgca tcatagtctt ccctcaagaa caaatcaggc
    22801 cagcatttgc tggccccaga ttgataacaa agtgcgcgtt ttccatgccg gatgcggcgt
    22861 aaacgcctta tccggcctac tcgatcgtgc atctttgtag gcctgataag cgtagcacat
    22921 caggcagttt tgcatttgtc atcgcccgta tgctttctta gtcccaccaa acgtcgaaaa
    22981 gttcgctggt gcgtacctct tccagtttgc gttcttccag ccacttacgc acaatcgcct
    23041 gatgttcttc ggtgcatttg ccgatttcct gcatgcagat cagaccttcc caggccagat
    23101 aaccgctgcc gtcaaaggcc agtttgttcg gttcgataac ctcgttaata aagtcatcaa
    23161 cggttttatc aatctgttct tccgatgtac cttccgggaa tcgccatgcc accgaaaatc
    23221 ctaattcctg gaattcgtcg atgtgcattt ttttacgcag acgacggcta cggttctttg
    23281 ccattatttc accctctcga acattaagtc ccatactccg tgaccaagac gatgaccacg
    23341 ttgttcaaat ttcgtcaccg gacgtgatgc cggacgcggt acgtaatcat tgctctctga
    23401 caggttttta taaccgtcaa tagaagacat cacttcaagc atatgttccg cataaggttc
    23461 ccagtcggtc gccatatgga atatgccccc cagctgcagt ttgcttttta ccagctcggc
    23521 aaacggcacc tgaacgatac ggcgtttatt atggcgcgct ttgtgccacg ggtcagggaa
    23581 aaagagctgc accatgcgca atgaattgtc aggaatcatt ttatgcagca cttcaaccgc
    23641 atcgtgacac atcacgcgca ggttgcttaa gccctcttca tgcgcagaag acaggcacgc
    23701 accaacgccc ggtgaatgca cttcaatgcc gaggaagtcc tgctcggggc gatctttagc
    23761 cattgccacc aacgacgcgc ccatgccaaa accaatctca agcgtcaccg gcgcttcacg
    23821 gccaaaaagc gcagggaaat ccagcatatc ttcgctgaac tcaacgccca tcaccggcca
    23881 gtagttttcc agcgcatgtt cctggccttt ggtcagtcgc ccctggcggc gcacaaaact
    23941 acggatacgg cgcagcgggc ggccgttttc atcaaattcc ggtgagatga cgtcgttttt
    24001 cataaagatt tagtcgcttg tgaaagtgtt ctgaaaacgg gcattatcca aagttagttg
    24061 ccggatgcaa gcatgataag gccgtggctg cggaaagttc cggtttacac cctgccgtcg
    24121 ctgtgctgca atcttgcccc caacaacagt gaattcggtg accatgcaag cgtcgcaatt
    24181 ttcagcccag gttctggact ggtacgataa atacgggcgg aaaacgctgc cctggcaaat
    24241 tgacaagacg ccctacaaag tatggctctc agaagtgatg ttgcaacaaa ctcaggttgc
    24301 gaccgttatc ccctattttg aacgctttat ggtgcgcttc ccaacggtga ccgatctcgc
    24361 caatgcgccg ctcgacgaag ttctccactt gtggaccggg cttggctatt acgcccgagc
    24421 gcgcaatctg cataaagcgg cacaacaagt ggcggcctta cacggcggta aattcccgga
    24481 aacctttgag gaagttgcag cactgccggg cgtcgggcgt tccaccgcag gcgcgattct
    24541 ctcgctttct ctgggtaagc actttccgat tctcgacggt aacgtcaaac gcgtgctggc
    24601 gcgctgctat gctgtaagcg gctggcccgg gaaaaaagag gtcgagaata aactatggag
    24661 tttgagcgag caggtgacgc ccgcggttgg cgtggaacgg tttaaccagg cgatgatgga
    24721 tttgggcgcg atgatttgta cgcgctctaa gccgaaatgc tcgctctgtc cgctacaaaa
    24781 cggatgtatt gccgccgcca acaatagctg gtcgctttat ccgggcaaaa aaccgaaaca
    24841 gacgctgcca gagcgcaccg gctacttttt gctgttacag cacgaagatg aagtattgct
    24901 ggcgcagcgt ccgccgagcg gattgtgggg cggtttatac tgtttcccgc agtttgccga
    24961 tgaagaaagt ttgcggcagt ggctggcgca acgacagatt gctgccgata acctgacgca
    25021 gctgaccgcg tttcggcata ccttcagcca tttccactta gatattgtgc ctatgtggct
    25081 tcccgtgtcg tcattcaccg gctgcatgga tgaagacaat gcgctctggt ataacttagc
    25141 gcaaccgccg tcagttggcc tggcggctcc cgtggagcgt ttgttacagc agttacgcac
    25201 tggcgcgccg gtttagcgcg tgagtcgata aagaggatga tttatgagca gaacgatttt
    25261 ttgtactttc ctgcaacgtg aagcagaagg tcaggatttt cagctgtacc ccggcgaact
    25321 gggaaaacgc atctataacg agatctccaa agaagcctgg gcgcagtggc agcacaaaca
    25381 aaccatgctg attaatgaaa agaaactcaa catgatgaat gccgagcacc gcaagctgct
    25441 ggagcaggag atggtcaact tcctgttcga gggtaaagag gtgcatatcg agggctttac
    25501 gccggaagat aaaaaataaa aacagtgccg gagcacgcct ccggcaactt gcataaaaac
    25561 aaacacaaca cgcacccgga atgatgaaaa aatatctcgc gctggctttg attgcgccgt
    25621 tgctcatctc ctgttcgacg accaaaaaag gcgataccta taacgaagcc tgggtcaaag
    25681 ataccaacgg ttttgatatt ctgatggggc aatttgccca caatattgag aacatctggg
    25741 gcttcaaaga ggtggtgatc gccggtccta aggactacgt gaaatacacc gatcaatatc
    25801 agacccgcag ccacatcaac ttcgatgacg gtacgattac tatcgaaacc atcgccggga
    25861 cagaacctgc cgcgcatttg cgccgggcaa taatcaaaac gttgctgatg ggggacgatc
    25921 cgagttcggt cgatctctat tccgacgttg atgatattac gatttcgaaa gaacctttcc
    25981 tttacggtca ggtggtggac aacaccgggc agccgattcg ctgggaaggt cgcgcgagca
    26041 acttcgcgga ttatctgctg aaaaaccgtc tgaagagccg cagcaacggg ctgcgtatca
    26101 tctacagcgt caccattaac atggtgccga accaccttga taaacgtgcg cacaaatatc
    26161 tcggcatggt ccgccaggca tcacggaaat atggcgttga tgagtcgctg attctggcaa
    26221 ttatgcagac tgaatcttcc tttaacccgt atgcggtcag ccgttccgat gcgctgggat
    26281 taatgcaggt ggtacaacat accgccggga aagatgtgtt ccgctcgcag gggaaatccg
    26341 gcacgccgag ccgcagtttc ttgtttgatc ctgccagcaa tattgatacc ggcaccgcgt
    26401 atctggcgat gctgaacaac gtttatctcg gcggaattga taacccaaca tcgcggcgtt
    26461 atgccgtcat caccgcctat aacggcggcg caggcagcgt gctacgagtc ttttcgaatg
    26521 ataagattca ggctgccaat attattaaca ccatgacgcc gggcgatgtt tatcagacgc
    26581 tgacgacccg ccatccctct gcggaatctc gccgttatct ttataaagtg aataccgcgc
    26641 aaaaatctta ccgccgccga taattccact aaccgcccct gacgatgctc aggggtaaaa
    26701 atgttatcta catcacaatt tcgttttgca aattgggaat gtttgcaatt atttgccaca
    26761 ggtaacaaaa aaccagtccg cgaagttgat agaatcgcat catctcgcac ggtcaaatgt
    26821 gctttttcaa acactcatcc gcatcacgat gtgaggaaat taacatgaat cttaagctgc
    26881 agctgaaaat cctctctttt ctgcagttct gtctgtgggg aagttggctg acgaccctcg
    26941 gctcctatat gtttgttacc ctgaagtttg acggtgcttc tatcggcgca gtttatagct
    27001 cactgggtat cgccgcggtc tttatgcctg cgctgctggg gattgtggcc gacaaatggt
    27061 taagtgcgaa atgggtctat gccatttgcc acaccattgg cgctatcacg ctgttcatgg
    27121 cggcagaggt cactacgccg gaggcgatgt tccttgtgat attgattaac tcgtttgctt
    27181 atatgccaac gcttgggtta atcaacacca tctcttacta tcgcctgcaa aatgccggga
    27241 tggatatcgt tactgacttc ccgccaatcc gtatctgggg caccatcggc tttatcatgg
    27301 caatgtgggt ggtgagcctg tccggcttcg aattaagcca catgcagctg tatattggcg
    27361 cagcgctttc cgccattctg gttctgttta ccctgactct gccgcatatt ccggttgcta
    27421 aacagcaagc gaatcagagc tggacaaccc tgctgggcct cgatgcattc gcgctgttta
    27481 aaaacaagcg tatggcaatc ttcttcatct tctcaatgct gctgggcgcg gaactgcaga
    27541 ttaccaacat gttcggtaat accttcctgc acagctttga caaagatccg atgtttgcca
    27601 gcagcttcat cgtgcagcat gcgtcaatca tcatgtcgat ttcgcagatc tctgaaaccc
    27661 tgttcattct gaccatcccg ttcttcttaa gccgctacgg tattaagaac gtaatgatga
    27721 tcagtattgt ggcgtggatc ctgcgttttg ctctgtttgc ttacggcgac ccgactccgt
    27781 tcggtactgt actgctggta ctgtcgatga tcgtttacgg ttgcgcattc gacttcttca
    27841 acatctctgg ttcggtgttt gtcgaaaaag aagttagccc ggcaattcgc gccagcgcgc
    27901 aggggatgtt cctgatgatg accaacggct tcggctgtat cctcggcggc atcgtgagcg
    27961 gtaaagtggt tgagatgtac acccaaaacg gcactaccga ctggcagacc gtatggctga
    28021 ttttcgcagg ttactccgtg gtgctggcct tcgcgttcat ggcgatgttc aaatataaac
    28081 acgttcgtgt cccgacaggt acacagacgg ttagccacta attacgcaaa gaaaaacggg
    28141 tcgccagaag gtgacccgtt tttttattct tacttcaaca cataaccgta caaccgtttc
    28201 atgccatccg catcggtttc gctatagaca ccttgcaact ccggcgaaaa tcccggcaac
    28261 aaattcacgc cttcttccag cgcaaggaaa taacgttgaa ccgccccacc ccagacttcc
    28321 ccgggtacca cgcaaagcac gccaggtgga taaggcaacg ccccttctgc cgcaattcgc
    28381 ccttcggcat cacgaatccg caccaactcc acttcaccgc gaatataagc gctatgcgca
    28441 tcctggggat tcatcaccac tgacgggaaa ctctgctggc ggaacatcgc tttttgtagg
    28501 tctttgacgt cgaaactgac atacaaatcg tgcatctcct gacacaactg gcgcagggtg
    28561 tagtcgcgat agcgcaccgg atatttgtta taaacgctcg gcaacacctc agccagcggc
    28621 gaatcatcct caatatgctg ttcaaactgc gccagcatcg ccaccagttg tgccagcttc
    28681 tcgtggcttt ccgccggagt taataagaac agaatggagt tgagatcgca cttctccggc
    28741 acaatgccgt tctcacgcag gtagtgcgcc agaatcgtcg ccggaacgcc aaagtcgcta
    28801 tattcgccgg tttcggcatc gatacccggc gtggtgagta acagcttgca cggatcaaca
    28861 aaatactgat ccgcggcata tccttcaaag ccgtgccaat tcgcccccgg ctcaaaactg
    28921 aaaaaacggc ggtcgctggc taacactgac gtcggataat cctgccacaa tttgccatca
    28981 acaacgggcg ggataaacgg gcggaacagc ttacagcgcg caagaatagc cttgcgcgac
    29041 tcaataccca actcaacgca ctcagcccac agccgacgcc cactctcccc ttcatgaatt
    29101 ttggcgttaa cgtccagggc ggcgaacagc ggatagaacg ggctggtaga agcatggagc
    29161 ataaaggcat tattcaaccg cttatgcggg caaaaacgcg cctgtccacg gatatggtta
    29221 tcttttttat ggatctgcga cgtctgtgag aatcccgcct gctgtttgtg caccgactga
    29281 gtcacaaaga tccccggatc gttttcgtta agttctaaca gcagcggcga gctatccgcc
    29341 atcatcggga taaactgttc gtaaccgacc cacgcggaat caaacagaat gtaatcacac
    29401 agatgcccaa cggtatcgat cacctgacgg gcgttataga cagtgccgtc ataggttccc
    29461 agctgaataa tcgccaggcg aaacgggcgc ggcaggtcgg ctttttctgg cgcaacgtcg
    29521 cgaatttgct ggcgcagata ctcttcatta aaacagtgcg catcaatacc gccaatgaaa
    29581 ccaaacgggt tacgtgaagc ttccagatag accggtgtcg cccccgcctg aatcaacgcg
    29641 ccatgatgat tcgacttatg gttgttacgg tcgaagagca ccagatcgcc acgcgttaac
    29701 agcgcattcg tcaccacttt attcgctgcc gatgtgccgt tcagcacaaa ataggtttta
    29761 tcggcatgaa agactttggc tgcaaatttc tgcgcatctt tcgccgatcc ttcatgaata
    29821 agcagatcgc ccaattttac gtcagcgtta cacatatcgg cgcgaaagat gttctcgcca
    29881 aagaaatcgt aaaaatggcg tccggcagga tgctttttaa agaacgcacc atgttgatgt
    29941 ccagggcaag caaaggtgct attacccatc tcaacgtact gcgtcagcgt gtcataaaac
    30001 ggtggcagca aattctcttc atactgacag gctgcggatt ccagctccag ccactgctgc
    30061 tcgttgccgt tgattaccgc cgtaacgccc gcaggtaatt caacagcatg ttcggaatac
    30121 aaaaacaccg gtagatgaaa accggtgcgc ttaagcaatg caagaatgcc actgcgacta
    30181 tccgcagcgg taatgacgac tgccgcgacg tccgtaaaat cagtatctcc caacgccacc
    30241 acgcggcgat gagtagaaag tcgggatacc agttcactac tggcggcaat attcattgat
    30301 ttcataagcg caaaacccgt ttaggggaag taagaatacc ggacaaggag gaaaaccctg
    30361 ccccatgaga tatgggtcaa actggtcacc agctccgacc gccagacatc agtaaaagca
    30421 gaaacgctct gattttactg ttgtcctgca gtgagcgtgc gttaacttca ccgcatgagc
    30481 agtagcataa aaagagaaga gtttcgcgcg aaacggcgat aagcgagaga atgtaaggag
    30541 gtggcgtgca tcgggcaaac tccgtacaga gaggagaaaa ttcgcgcaat catggcacct
    30601 ttcgctaaag cgtgcaagcc aggactttgc gaacaaacaa gccatcgacg gcgccaaaag
    30661 ttcataataa gaaaatcaaa caatacagct aacaggaact cttgtggtca tcggcccttt
    30721 tatcaacgca agtgcagtct tactgggtgg cgttttcggc gcacttctta gccaacgctt
    30781 accggaacgt atccgcgtct ccatgacatc aatttttggt ctggcatcgc tggggattgg
    30841 tattttactg gtggtgaaat gcgccaacct tccggcgatg gttttagcaa ccttactcgg
    30901 agcattaatc ggcgaaattt gtctgctgga aaaaggcgtc aatacagtgg tcgccaaagc
    30961 gcataatctg tttcaccact cgcgtaagaa gccagcgcat gaatctttta ttcagaattt
    31021 tgtcgcgatt attgtcctgt tttgcgccag cggcaccgga atcttcgggg cgatgaacga
    31081 agggatgacc ggcgatccga gtattttaat cgccaagtca tttcttgatt tctttacggc
    31141 gatgatcttc gcctgctcgc tgggtattgc ggtgtcggtg attagcattc cattactgat
    31201 catccagtta acactggcgt gggctgccgc gctgatatta ccgctgacca caccgtcgat
    31261 gatggcagac ttcagcgccg taggcggttt attgctgctg gcaaccggat tacgcatctg
    31321 tggcattaaa atgttcccgg tggtcaacat gcttccagca ctccttctgg caatgccgct
    31381 ttccgccgcc tggaccgcct ggtttgcctg acaatgcgtg caatagcggc aaagtgatga
    31441 tagattgtgc agtctgcact aaattgaaga aatttgattg acgagacgag gcgaatcagg
    31501 tttaa
//
LOCUS       DAOTRK010000048        31319 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.048, whole genome shotgun sequence.
ACCESSION   DAOTRK010000048 DAOTRK010000000
VERSION     DAOTRK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.048"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..3857)
                     /gene="entF"
                     /locus_tag="QIE41_002083"
     CDS             complement(<1..3857)
                     /gene="entF"
                     /locus_tag="QIE41_002083"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDS3530973.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALVDASYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTNLCYNAPLTPQGSAPLQLSQPHNTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTTALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQIAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFIGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGP"
     gene            complement(3854..4072)
                     /gene="ybdZ"
                     /locus_tag="QIE41_002084"
     CDS             complement(3854..4072)
                     /gene="ybdZ"
                     /locus_tag="QIE41_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDS3530974.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQGAQ"
     gene            complement(4075..5277)
                     /gene="fes"
                     /locus_tag="QIE41_002085"
     CDS             complement(4075..5277)
                     /gene="fes"
                     /locus_tag="QIE41_002085"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDS3530975.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERVDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSTLEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKDIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5520..7760
                     /gene="fepA"
                     /locus_tag="QIE41_002086"
     CDS             5520..7760
                     /gene="fepA"
                     /locus_tag="QIE41_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDS3530976.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            7935..8555
                     /gene="entD"
                     /locus_tag="QIE41_002087"
     CDS             7935..8555
                     /gene="entD"
                     /locus_tag="QIE41_002087"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDS3530977.1"
                     /translation="MKTTHTALPFAGNTLHFVKFDPASFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGATALAVVSRQ
                     PIGIDIEEIFSAQTAAELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASKI
                     QAAQGFLDYQIISWNKQQIIIRLEDEQFAVHWQIKEKIVITLCQHD"
     gene            complement(8677..8829)
                     /gene="hokE"
                     /locus_tag="QIE41_002088"
     CDS             complement(8677..8829)
                     /gene="hokE"
                     /locus_tag="QIE41_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDS3530978.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVRDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9272..10390
                     /locus_tag="QIE41_002089"
     CDS             9272..10390
                     /locus_tag="QIE41_002089"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDS3530979.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEDTLRLLEKIVPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10456..10704
                     /locus_tag="QIE41_002090"
     CDS             10456..10704
                     /locus_tag="QIE41_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDS3530980.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10769..11137
                     /locus_tag="QIE41_002091"
     CDS             10769..11137
                     /locus_tag="QIE41_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDS3530981.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11231..11884
                     /gene="nfsB"
                     /locus_tag="QIE41_002092"
     CDS             11231..11884
                     /gene="nfsB"
                     /locus_tag="QIE41_002092"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDS3530982.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRRFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            11992..13239
                     /gene="ybdG"
                     /locus_tag="QIE41_002093"
     CDS             11992..13239
                     /gene="ybdG"
                     /locus_tag="QIE41_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDS3530983.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHKEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13320..14696)
                     /gene="pheP"
                     /locus_tag="QIE41_002094"
     CDS             complement(13320..14696)
                     /gene="pheP"
                     /locus_tag="QIE41_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDS3530984.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14798..17941)
                     /gene="cusA"
                     /locus_tag="QIE41_002095"
     CDS             complement(14798..17941)
                     /gene="cusA"
                     /locus_tag="QIE41_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDS3530985.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAAIMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(17953..19176)
                     /gene="cusB"
                     /locus_tag="QIE41_002096"
     CDS             complement(17953..19176)
                     /gene="cusB"
                     /locus_tag="QIE41_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDS3530986.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDRAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19192..19524)
                     /gene="cusF"
                     /locus_tag="QIE41_002097"
     CDS             complement(19192..19524)
                     /gene="cusF"
                     /locus_tag="QIE41_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDS3530987.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKITIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19548..20930)
                     /gene="cusC"
                     /locus_tag="QIE41_002098"
     CDS             complement(19548..20930)
                     /gene="cusC"
                     /locus_tag="QIE41_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDS3530988.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNVSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21087..21770
                     /gene="cusR"
                     /locus_tag="QIE41_002099"
     CDS             21087..21770
                     /gene="cusR"
                     /locus_tag="QIE41_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDS3530989.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21760..23208
                     /gene="cusS"
                     /locus_tag="QIE41_002100"
     CDS             21760..23208
                     /gene="cusS"
                     /locus_tag="QIE41_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDS3530990.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLKRVLNHPEETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            23945..25846
                     /locus_tag="QIE41_002101"
     CDS             23945..25846
                     /locus_tag="QIE41_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDS3530991.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHHETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            25874..26335
                     /locus_tag="QIE41_002102"
     CDS             25874..26335
                     /locus_tag="QIE41_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDS3530992.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            26355..31187
                     /locus_tag="QIE41_002103"
     CDS             26355..31187
                     /locus_tag="QIE41_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HDS3530993.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPNDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPD
                     GNILREAAPDGSTTMYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERTGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYVLDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTTYRYNAAGELAEVILPEDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQLTSQRAGLFPAEATWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGWHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRIKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRITYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQCSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDAEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLLGGEQTYRYVPNPCGYVDPLGLAFCPRMQKTLQKLTDKAVADIIAN
                     PRLAEDLMSAGSYAHLKNGTNLYAASFGKAVERRVAQLVSDNSRLSKLVEHTGQAKGA
                     NGQFISSPDFTTKGKGVFDVTTNKALQAHIDRYTKAGVYTPYNDIGYLVYDVVYGLSF
                     "
BASE COUNT         7563 a         8435 c         8299 g         7022 t
ORIGIN      
        1 atcggcccaa ttttttcaaa cgcccctgga gagataatat ccacatgcgc acaatcctga
       61 cgatagatat ccagctccgc aatccacggc gaccaggcgc gttcgggatt cataccttcc
      121 tgaagcgtgc gttcagcaac aaacagcgtc gctttaccgt caaacggtac actatgagca
      181 gtcgtcagca ggcgcacagc atcagcgtag ttgccttcaa tggtggtaaa caactccgtt
      241 gaagtacttc cctgctgtgc cgccaggaag gcctcgcgct cgcggttaat ctccgccagc
      301 acttccgggt ccaggccatt agcctctttt tcctgccagt tttgcgtttc tggcggccag
      361 gtatccagca agccaataaa tgccacctgt tcgccacggg cacgcagccg cgccgcaatg
      421 ccctgcgcca gcgtaccgcc cagcgaatac cccagcaggt aataagggcc gtgcggttgt
      481 tgttcaagta acgttgccag atgcgcttcg cagacttcat ccaggtttgc cgcgatctgc
      541 atggggccat gagggcgcgg cgactgaatg ccgataatcg accattgtgg atcgagataa
      601 cgcgagagca cgctgaactg ccaggcaaaa ccggacgcag gatggaaaca aaacagcgtc
      661 gggccattac cttcacgcaa cggcagaatg gtttcgaatc ccatgcgccg ggagctgtct
      721 tcttcaccat caataatcgt tgccagtttg gcgacagttg acgcgaccat cacctgcccc
      781 ggcgtcacct gacgggcaaa ctgccgactt aactgcgcag ccagtttcat tgccagtagc
      841 gaatgaccgc caagcgcaaa gaaatcagca tcggcatcct gcacgtcaca acccagcaac
      901 gacgagaatg ccgcggcgat aatcgtttca ctgcccgctt tcggcgcacg ccccggcgct
      961 tgtgctttca gttcaggcaa cggtaaggct ttgcgatcca gcttgccgtt ggcgctaagc
     1021 ggcaactgcg gaagttgcag cagaaccacg ggcaccatat gcggtggcaa tgtttcgcgc
     1081 aactgcgcct gcaatgcagt ggtatccagc ggcaggcctg attgcgacac cagataccca
     1141 accaactgac gcgcatcgcc tcccgttgcg gcagcctggt taatgacgca ggcgtgggtc
     1201 acggcctgtt caacgtccgg cagcgcctgc atcacgcgat cgatttcgcc cagttcaata
     1261 cgctgcccgc gaatttttag ctgatcgtca ctgcgcccga ggtactccac cgcgccgtta
     1321 tccagccagc gggcaacgtc tccggtacgg tacatccgtt cccctggagc aaaaggatcg
     1381 gcaataaagc gactggcggt cagatcgggt cgtcccagat acccctgcgc cagttgaata
     1441 ccggtgagat agagatcacc cgccacacct ggcggcaccg gatgcatcat cgcatcaaga
     1501 atacgcaggc ccgtattcca caccggataa ccaatcggca cactactgcc gcgcacctct
     1561 gccagttcct cgccaaaagc cggataccag ctgacatcta ccgccgcttc cgtcgggcca
     1621 tatagattat gcaacggcac gctggttaac tgttgccatt cgcggcataa atcagctggt
     1681 aaggcctcac cactacagaa aacctgtttc aacgtcgcgc aactctggcg agcggtttgc
     1741 ggcgtcagcg atgcaacaaa tgccgccagc atcgacggca caaagtgcgt agtcgttacg
     1801 ccatattcgg caaagaattg ctgcatagcg agcggatcgc ggtgcgcttc cggttcagcc
     1861 atcaccagtt tagccccggc aataaacggc cagaaaaact cccacaccga gacatcaaaa
     1921 ctgcacggcg ttttttgggc aacgacatct tcacctgtaa gtggataatg attttgcatc
     1981 cacaacaggc ggttaacgat agccgtctgc ccgaccatta cccctttcgg cctgccggtg
     2041 gagccagagg taaagatgat ataagccgtg ttatgcggtt gtgaaagttg cagcggcgca
     2101 ctgccctggg gggtaagcgg ggcgttatag caaaggtttg ttaaattggg aacatcgcta
     2161 aagcgcggca gttgatcgtc ggtggtaatt aacagcgacg gacgcgcatc ttccagcatc
     2221 attttcaggc gatcgtccgg atagccggta tccagcggta accaggccgc accagcctca
     2281 acaattgcat gtagcgccag agtcaaaaat accgagcgcg gtagcgccac cgccacgctg
     2341 tcgcctggtt taacgccgcg ctcacgcagc agattcgcca gcgccaccac ctgctcgcgc
     2401 atttcccgat agctaaactg gtaactcgca tcaaccagcg ccggagcatg cggtgttttt
     2461 gccgcttgtt ctgccaccag cgcgctcagc gtggtttctg gaatctcaac ctgagtggcg
     2521 ttgatctgcg ccagctgcgc gtattcacct ggcagcataa tatcgacatc gccgcacaac
     2581 agcgccggat ccgcggcgaa ctgggcaatc agcattttca ggcgttcagc gtgttgaatt
     2641 aacgttggct catcgtaacg ctgtttattg gcgaggatct caatactcaa atcaccgtgt
     2701 tcatccggga acagtgccag ttcaaggtca ttaaccggac cggttgccag ggtatgggtt
     2761 tgcgcctgaa caccaggaat atccagttgg taatcaaata ccttgatatt gagtaccgga
     2821 ccgaacagcg gttcctctcc tgccgctcgc ccgctgtcac ggacaatttg ttcggcatcg
     2881 taacgttgat gacgacgcat ttttttcagt tgtgctgcca gtcgggttgc cagttccggc
     2941 agcgtttctt gtgccgcaat gtgaataccc aacggcaaaa cgttgagcac gggtccggta
     3001 gccgtcagcg ccgccgagcc cagtcgacgc ataaagataa atccggcagc gtagtccatg
     3061 cggttgcaca atcgccccag ccacaaagcc gccagcgcaa gggctaaatc ggtacgctgc
     3121 acacctgaaa gttgcgtagc cagctggcgg aattccccgt cagtaaattc cagtttcagg
     3181 cgaagaatat ctgccgaagc gctgcgcccc ggtaaaggtg ccggagaaag tgacgcgggc
     3241 ggcggcagtt gacgacgctg ttccgcccag aatgccgcat cacgctgcca ggcttcgctt
     3301 tcgcggtatt gctggtactc ttccactaca tcagcgaaag gcgtaaacgg cgaagcaggc
     3361 gttggttcgc cacgcagcca tgcgcagtaa atattggcga tctggcgtgt aatggccggg
     3421 aaactgaagc catcgaccag caaatggtga taacgctgat accagtacca gcggttatcc
     3481 gccacctgta tcaactgatg aaagaccagt ggtttaccgc tatcgacgcg caaatcttgt
     3541 tgcaaatccg cctgcattaa tgcccgcgca gtaccgtgcg gatcaatgtt ggttcgcagg
     3601 tcgataattt ctggcaattc gaacgtctgc gcatcatcga cccactgcca gacttcgccg
     3661 ttatcttccg taaaacgcat ccgcaacgta tctgcttgcg ctagtccggc aaccaccgcg
     3721 cgggccagta gtggcgcatc aacctctccg gttaactcaa cgtaatgcgc cacgctccag
     3781 gcggagggta aatctgacag tttttctgcc atccagatgc cgggctgtgc ggcgaccaaa
     3841 ggtaaatgct ggctcattgt gccccctgca actgggtgaa attcgccggt gtcagagtac
     3901 gccagtgggc ttcaagccac tgctggcagg acgcctgtga ctgcggctga cacacaacgt
     3961 cccagcctgc cggtaagagg cattgttgcg gccacagact gaattgcccc tgcgcattgc
     4021 gcaaaatgta aaacgctccc tgcggatcat cgaagggatt actgaatgcc atattcaact
     4081 cctgtcatgg aaaagtggtt gccagaggtc gattagcccc tgcatcaagc caccgcgcca
     4141 acaaagcgca tcatgtccgc cgtcaacctg acgccagaaa atggattctt ttatggggtg
     4201 taattgcgca tacagcgcct gattggcctg catgatcatc ggctcgcgaa tacccgcttc
     4261 cagcacaatg cgcagacctt cggcactaac ttgaccagct ttaagttgct caagtaaaac
     4321 accttcttgc tgcccgcccc gatgcggcca ccagtacgat cctgactggc ttaatacaca
     4381 gccaaagcgt tcaggccagt gcagtccggc atacagcgcg gaaagcccac caaaactctg
     4441 cccggcgacc acggtgcgat cggcgcgatc gctaaaaggg gcaatatctt tcaccagggg
     4501 taataactct tgctgtactg ccagccagaa atccgcatta cacggcagtt cgtgggcgcg
     4561 gtgcgtggtg tcgatagcgt cgatcaacac atacacggcg ggaggaagtt gccgacgatg
     4621 ggtcagcgaa gttagcgccg gccagacggg catactctgc gcccaaaatt cgccatcaag
     4681 caaaactgcc agcgggcgtt cttctgctgt tgcatcgccg gtggtaaaaa tccatacacg
     4741 gcgtgaattt ttcagccgct cacttttcca gataatttct ttggcaggcg tttctggcgc
     4801 ttgcggacaa tcccaaccag gttgtagggg cgcttgcggc atttcgagtg tagaaacagc
     4861 gtgccctcgc ccgcctttcc agctttgtgg attcagcgga tcggctatcg cctggggtaa
     4921 tagttttcgc cagccttcgc gtaattcgag gcgatcgggg gatggtgcag aaaaaatgtc
     4981 aacgcgttcg gtgggaataa aacagtagct gccgcgccag ttggcattaa gttgtgtcgt
     5041 ccactgccag acgttggtac ctgcaattcg ctgcatcgac tggggctggc tgttctgatg
     5101 gtgatcggtc acaccagtga tgtagaccca tacgcgcttt atcgtcgagt attcttcaga
     5161 accctgggga tcacgccacc agaaagtgac ctcaaacatt tcgtcattca gacgctgcca
     5221 ttccgggcca tgtttcgact gccaccagct ctcacttcct acttttaacg ccgtcacacc
     5281 ataaccccat gtttactgtg aaatttttca ttgattgcag aaatatattg ataatattat
     5341 tgataactat ttgcatttgc aatagcgtaa tggcgcgccg tgggaagcgc ggacattaat
     5401 taaccaactg cactgcgtgt ctttcaggat caaaggtttt cgcggtagcg ggatgcgtcg
     5461 tgttgatgac gaccatgccc gacagttgca attcgtggca aaaatgcagg aataaaacaa
     5521 tgaacaagaa gattcattcc ctggccttgt tggtcaatct ggggatttat ggggtagcac
     5581 aggcacaaga gccaaccgat actcctgttt cacatgacga taccattgtc gttaccgccg
     5641 ccgagcagaa cttgcaggca cctggcgttt cgaccatcac cgcagatgaa atccgcaaaa
     5701 acccggttgc ccgcgatgtg tcggagatca tccgtaccat gccaggcgtt aacctgaccg
     5761 gtaactccac cagtggtcag cgtgggaata accgacagat tgatattcgc ggcatgggtc
     5821 cggaaaacac gctgattttg attgacggca agccggtaag cagccgtaac tcggtgcgtc
     5881 agggctggcg tggcgagcgc gatacccgtg gtgatacctc ctgggtgcca cctgaaatga
     5941 ttgaacgtat tgaagttctg cgtggtccgg cagctgcgcg ttatggcaac ggcgcggcgg
     6001 gcggcgtggt taacatcatt accaaaaaag gcagtggtga gtggcacggc tcgtgggacg
     6061 catatttcaa tgcgccagaa cataaagagg aaggtgccac caaacgcact aactttagcc
     6121 tcaccggtcc gctgggcgac gaattcagct tccgcttgta tggcaacctc gacaaaactc
     6181 aggctgacgc gtgggatatc aaccagggcc atcagtccgc gcgtgccgga acgtatgcca
     6241 cgacgttacc agccgggcgc gaaggggtga tcaataaaga tattaacggc gtggtgcgct
     6301 gggacttcgc accattacaa tcgctggaac tggaagcagg ttacagccgc cagggtaacc
     6361 tgtatgcggg cgatacccag aacaccaact ctgactccta tacccgctcg aaatatggcg
     6421 atgaaaccaa ccgtctgtat cgccagaact actcgctgac ctggaacggc ggctgggata
     6481 acggcgtgac caccagcaac tgggtgcagt acgaacacac ccgtaactcg cgtattccgg
     6541 aaggtctggc gggcggtacc gaagggaaat ttaacgaaaa agcgacacag gatttcgtcg
     6601 atatcgatct cgatgacgtg atgctgcaca gcgaagttaa cctgccgatt gatttcctcg
     6661 ttaaccagac gctgacgctg ggtacggagt ggaatcagca gcggatgaag gacttgagtt
     6721 ccaacaccca ggcgctcacc gggacgaata ccggcggcgc tattgatggc gtgagtgcca
     6781 ccgaccgtag cccgtattca aaagcagaaa ttttctcgct gtttgccgaa aacaatatgg
     6841 agctgactga cagcaccatc gtaacgccgg ggctgcgttt cgatcatcac agcattgttg
     6901 gcaataactg gagtccggct ctaaacatat cgcaaggttt aggcgatgac ttcacgctga
     6961 aaatgggcat tgcccgtgct tataaagcgc cgagtctgta ccagactaac ccaaactaca
     7021 ttctctacag taaaggtcag ggctgctatg ccagcgcggg cggctgctat ctgcaaggta
     7081 atgatgacct gaaagcggaa accagcatca acaaagagat tggtctggag ttcaaacgcg
     7141 acggctggct ggcgggtgtg acctggttcc gtaacgatta tcgcaataag attgaagcgg
     7201 gctatgtggc tgtagggcaa aacgcagtcg gcaccgatct ctatcagtgg gataacgtac
     7261 cgaaagcggt ggttgaaggt ctggaaggat cgttaaacgt accggttagc gaaacggtga
     7321 tgtggaccaa taacatcact tatatgctga agagtgaaaa caaaaccacg ggcgaccgtt
     7381 tgtcgatcat cccggagtat acgttgaact caacgctgag ctggcaggca cgggaagatt
     7441 tgtcgatgca aacaaccttc acctggtacg gcaagcagca gccgaagaag tacaactata
     7501 aaggtcagcc agcggttgga ccggaaacca aagaaatcag tccgtacagc attgtgggtc
     7561 tgagcgcgac ctgggatgtg acgaagaatg tcagtctgac cggcggcgtg gacaacctgt
     7621 tcgacaaacg tttgtggcgt gcaggtaatg cccagaccac gggcgatctg gcaggagcca
     7681 actatatcgc cggtgccggg gcgtatacct ataacgagcc gggacgtacg tggtatatga
     7741 gcattaatac tcacttctga tgctaacgtc agattgttga caaagtgcgc gtagttcatg
     7801 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaatc ttgccaattc aatatattgc
     7861 aggaccacgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg tcatcagtct
     7921 ctaatatggt cgacatgaaa actacgcata ccgccctccc ctttgccgga aatacgctgc
     7981 attttgttaa gttcgatccg gcgagttttt gtgagcagga tttactctgg ctgccacact
     8041 acgcgcaact gcaacacgct ggacgtaaac gtaaaacaga gcatttagcc ggacggatcg
     8101 ctgcagttta tgcgctgcgg gaatatggct ataaatgtgt gcccgcaatc ggcgagctac
     8161 gccaacctgt gtggcctgcg ggggtatacg gcagtattag ccactgtggg gctacggcat
     8221 tagccgtggt atctcgtcaa ccgattggca ttgatattga agaaattttt tctgcgcaaa
     8281 cagcggcaga attgacagac aacattatta caccagcaga acacgagcga ctcgcagaat
     8341 gcggtttaac cttttctctg gcgctgacac tggcattttc cgccaaagag agcgcattta
     8401 aggcaagcaa gatacaggcc gctcaaggtt ttctggatta tcagataatt agctggaata
     8461 aacagcagat catcattcga ctagaggatg agcagtttgc tgtgcactgg cagataaaag
     8521 aaaaaatagt cataacgctg tgccaacacg attaattgac aacatctggt acgattcgcc
     8581 cgcagccatt actgaccgcg ggcgaaagtg taaagcaggt gccttaccat cctgacctga
     8641 caaccggata tgcggggatt gctccccgca caacggctac ttcttcggtt cgtaagcgag
     8701 aacagcctta aactcaatat tacgttcctt caccgtaaac tcacacagcg agtcgcggac
     8761 cagaagcgta aatcccagca ccgttaaaca cagcactatg actgccacaa gggcatattt
     8821 cgtcagcatg tttatattgc ctccatgcga agaagggact accatccaaa ttgtttgggt
     8881 ttggagtggc agccccaggg ttgatagaaa tatcgtcctg gggcttcatc tttccagacc
     8941 tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttccttag
     9001 caaaataact gtttatttat acagtaagtc ctgtttcgga cttctcaatc ctcatttgat
     9061 caactgaagc tgaacccgtt tttttctgca tgcctcaatc gtcaaaattc ttcgccacca
     9121 gtccgcactg ccataacttg tcatattgca aaatgtagcc aatttgcttc agtcaccatc
     9181 atcaaaaaaa gaaacattcc agaacttccg catctcgccc agtcgctccc acttctatac
     9241 tgaatagaaa acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga
     9301 accttttacc ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt
     9361 aagccaggac tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt
     9421 aaagcacgat atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa
     9481 ccaggctgcc gggcaatttt cagcgatgca gaaagtcgta ttgcaggcag ccgcagatca
     9541 tcatctggaa atttgcggcg gtggcacgca cccgtttcag aaatggcaac gtcaggaggt
     9601 atgcgataac gaacgctatc aacgaacgct ggaaaacttt ggttatctca ttcaacaggc
     9661 gaccgttttt ggtcagcatg tccatgttgg ttgcgccagt ggcgatgatg ccatttattt
     9721 gctgcacggc ctgtcacggt ttgtgccgca ctttatcgcc ctttccgccg cgtcaccata
     9781 tatgcaagga acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc
     9841 cgataatggc ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg
     9901 cctgagttac accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag
     9961 tcctcatttt ggcacggtgg aggttcgggt aatggatacc ccgttaaccc ttagccatgc
    10021 ggtaaatatg gcaggattga ttcaggctac cgcccactgg ttactgacgg aacgcccgtt
    10081 taaacatcag gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta
    10141 tgggctggaa ggcgtcatta ccgatccgca tactggcgat cgtcggtcac taacggaaga
    10201 caccttgcgt ttactggaaa aaatcgtccc ttctgcacat aaaatgggtg catcgagtgc
    10261 gattgaggcc ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga
    10321 tttcgtcgcc gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc
    10381 tggtgactaa gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt
    10441 acatttaaca atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg
    10501 ctgatggctt tcctttcttg cctgctgcta cccgcccccg cactggggct gacgctggca
    10561 caaaaactgg tgaccatgtt ccatctgatg gatcttagcc agctttacac tttattgttt
    10621 tgtctgtggt ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga
    10681 cgctggttct cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc
    10741 ttattctcct tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa
    10801 acgcctggcc cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga
    10861 tgtttttaaa attggcggca agatttttat gctgtcgtcg gagctgcgcg gcgtcccctt
    10921 tatcaatctg aagtccgatc cacaaaaatc cctgttaaat cagcaaatat atccgagcat
    10981 taagccaggg tatcacatga ataaaaagca ctggatttcg gtgtatcccg gagaggaaat
    11041 ctccgaagcg ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct
    11101 ggctaaacgc gatcaaaaaa gagtgcgtcc aggctaaagc gggaatctat agcgcatttt
    11161 tctcgcttac catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca
    11221 tggagtcttt atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga
    11281 tgccagcaaa aaactaaccc cggaacaggc cgagcagatc aaaactctcc tgcaatacag
    11341 cccatccagc accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa
    11401 agcgcgtgtt gccaaatccg ctgccggtaa ttacgtgttc aacgagcgta aaatgcttga
    11461 tgcctcgcac gttgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct
    11521 ggttgttgac caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa
    11581 cgataaaggt cgcaggttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga
    11641 gtggatggca aaacaggttt atctcaacgt cggcaacttc ctgctcggcg tggcggcgct
    11701 gggtctggac gcggtaccca tcgaaggttt tgacgccgca atcctcgatg cagaatttgg
    11761 tctgaaagag aaaggctaca ccagtctggt ggttgttccg gtgggtcatc acagcgttga
    11821 agattttaac gctacgctgc cgaaatctcg tctgccgcaa aacatcacct taaccgaagt
    11881 gtaattctct cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca
    11941 taaccctgtt tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat
    12001 ttaatatccc aggttgaaga tttagcgggt attgagattg atcacaccac ctcaatggtg
    12061 atgattttcg gtattatttt tctgaccgcc gtcgtggtgc atattttttt gcattgggtg
    12121 gtactgcgga ccttcgaaaa acgtgccatc gccagttcac ggctatggtt gcaaatcatt
    12181 acccagaata aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt
    12241 caggcggtat tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg
    12301 cagttgtgga tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg
    12361 aatctggcgc agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg
    12421 attaaactga tcggcgcgat tctggtcggc attttgatga tctcgctgct gattggtcag
    12481 tcgccagcga ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt
    12541 aaagatccga ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa
    12601 ctgggcgact ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg
    12661 ttaaccaccg tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct
    12721 ctggtctctg actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt
    12781 aagcgcagta tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa
    12841 cgtctgaata aagcgcattt gttaaaacct tatttaacca gccgccacaa ggaaattaat
    12901 gagtggaatc gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat
    12961 attggaacct ttcgtgccta tctgaacgaa tatctgcgga atcatccgcg gattcgcaaa
    13021 gatatgacct taatggtgcg ccaattagct ccgggcgata acggtttacc gcttgagatc
    13081 tacgcgttta ctaataccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc
    13141 gatcacatat ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc
    13201 aatgatattc gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctcttatcg
    13261 aatggatgag agcggtttcg gatggttgac atcattttgt cggatacggc gtgaatgcct
    13321 tatttccgac gcagcgtttt aaacgctaca aacaggaata caatccacac tggcagcaga
    13381 atcgctgaca agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag
    13441 gcaatgcaga gatagttgcc gaacggataa agcagcgcct taaactgtgt ttcacgtccc
    13501 tgacgtcgca tcgccgcgcg aaaacgcaga tgcgccaggc agatcataat ccagttcaac
    13561 agcagcgttg ctaccaccag cgccatcagc agaccaaacg ctttttgcgg cagcagatag
    13621 ttgattaaca ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcacg
    13681 ccgcgacggc tgacgcgagt caaaaacttc ggcgcattac cctgcacaga aaggccaaac
    13741 agcatgcggc tgttagagta aaccccgctg ttatacaccg ataatgatgc taccagaatg
    13801 acgaagttca gcgcagaagc taccacgttg ctgtcgagat tatggaaaat catcacaaaa
    13861 gggctactgt tggatttcac ttccacccac ggatagagtg ccagtaaaac caccagtgaa
    13921 ccgatataaa acagcaggat gcgatacacc acctgattca ctgctttcgg gatgcttttt
    13981 tccggatcgc gcgcttcagc cgcagtaatc ccaatcagct ccagaccgcc gaaggagaac
    14041 ataattaccg ccagcgacaa aatcagccca ttccagccgg tggcgaagaa accaccgtag
    14101 cgccagaggt tgtcgatact ggctttttcg ccgccgtgcc cagaaaacag cagccacagg
    14161 ccaaagccga tcataccgat gatcgccagc actttaatca gcgcaaacca gaattcggtt
    14221 tcgccatata agcgcacgtt taccaggtta acggcgttga tgataataaa gaaggcggca
    14281 gcccaaatcc acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc
    14341 agctctgcca ttcccaccag cacgaacatc acccagtagt tccagccaga gaggaagccc
    14401 gcaaacggtc cccagtattt ataggcaaag tgggcaaatg aaccagatac cggctcttcg
    14461 accaccatct cgccgagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag
    14521 cccagcaata cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca
    14581 gtaccaattg cgccacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga
    14641 agcgtcggct cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct
    14701 ttcccctgtg tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca
    14761 agacacaatc cacacggtta aaccgggtat cctgctttta tttccgtacc cgatgtcggt
    14821 gcagccacat cagcttatat gccgccggga taataaacag cgatagcaaa ggcgcggtga
    14881 tcatgccgcc aatcatcggc gcggcaatcc ggctcatcac ctctgaacct gctcccgttc
    14941 cccacagaat cggcagcaga ccagcgataa tcaccgccac cgtcatcgct ttcgggcgca
    15001 cgcgcaggac cgcgccgtga tacaacgcct catccagctt ctgctcgctg aatgtttgcg
    15061 gattattcaa cgacggctcg gcctctatgg cgtgacgtaa atacatcagc atcaccacgc
    15121 caaattcggc ggcaaccccg gcgagggcaa taaaaccggt gcccgtcgcc acggaaagat
    15181 gaaagcccat ccaccacagc aaccagatgc cgcccaccag cgcaaacggt acgctgctga
    15241 taatcagcaa cgcttcgccc acccgacgga acgccagata taacagcacg aagatgatca
    15301 tcagcgtcat cggcaccatg agtttcagct tatggttggc gcgttccagt agctcgaact
    15361 gcccggagaa tgccacgctg gtgcccggtt ttaactgtac tttttcagct atcgcttttt
    15421 gcagatcgtg aaccaccgac accatgtcac gatcgcgggc atcgatataa atccagctcg
    15481 tcgggcgcgc attctcggtt ttcagcatcg atggtccggt agagacttta acgtcggcca
    15541 cgtctgccag ggtgatttgc tgcttcatcg gcgtcaggat cggcaactgg cgcagcgcct
    15601 gcggactatc gcgccagctt tgcggataac gcagattaat tggataacgg gcaatccctt
    15661 ccaccgtttc gccaaccatc gccccgccca ccgcagaagt cacaaacaat tgcacatccg
    15721 ccaccgtcat accgtaacgc gcggcttttt cacggttaat ctcaacgttg atatagcgcc
    15781 caccttccag acgctcagca agcgcagaag ctacgcctgg caccgttcgt gccacttctt
    15841 caatttgctc agccatcgcg tcgatatccg ccagcacagt accggaaact ttaatgccga
    15901 tggggctttt aatgccagtc gagagcatat cgatacggtt acgaattggc ggcacccaca
    15961 gattcgccag ccccggcagc cgcacggtgt tatccagttc ctcgatgatt ttgtccatcg
    16021 tcatgcctgg ccgccactgc tcttgtggct taagctggat ggtcgtttct accatctcca
    16081 gcggagcaga atcggtggcg gtttccgctt tgccggtttt gccaaatacc cgcgccactt
    16141 caggtacgct cataatcagc ttgtcggttt tttgcagcat aatcgccgcc tctgctgcgg
    16201 aaatccccgg cagcgtcgat ggcatataca acaagtcacc ttcattgatc tgcggtaaaa
    16261 actccccgcc aactttattg agcggccaga gaaccgtcag caccgaaagc gctgccacca
    16321 gcagcgtggt tttcggccag tgcagtactt tcagcaacag cggatgataa acacgaatca
    16381 aaaagcgatt gagcgggtta ctgctttccg gcggaatttt gccacggatc cagtagccca
    16441 tcaggatagg gatcactacg atcgccagca gcgccgcacc cgccatcgca tacgttttgg
    16501 tgaacgccag cgggccaaac agacgccctt cctgcccttc cagggtgaag atcgggataa
    16561 acgacaacgt gataatcagc agactgataa acagcgccgg ccccacttca acagatgcat
    16621 cggtgatcac ctgccagcgc gttttattat ccagcgtggc gtcaggatgc tggtgctgcc
    16681 actcttccag ccgtttatgc gcattctcga tcatgacgat agcggcatcg accatcgccc
    16741 cgacggcaat agcaataccg cccagcgaca taatattggc gttcagtccc tgaaagtgca
    16801 tgacaataaa agcaatacac aaccccagcg gcaacgaaat aatcgccacc agcgccgagc
    16861 gcacatgcca gagaaacagc gcacagacta ccgccaccac aataaactct tccagcaact
    16921 tgccgctgag gttgtcgatg gcgcggtcaa tgagctggct gcgatcgtat gttgtaacta
    16981 tctccacgcc ttccggcaga ctacttttca gcgtttccag tttgtctttc acggcggcga
    17041 tcacttctcg ggcgtttttg ccggagcgca ggatcaccac cccgcccgcc acttcgcctt
    17101 cgccgttcag ttcggcaatg ccccggcgca tttccgggcc aatctggacc ttcgcaacat
    17161 cacgcagata aacgggcacg ccattttcac tggcttttaa aacgatgtga ttaaagtcgt
    17221 cgagcgtttg cagatagccg ctggctcgca ccatatattc cgcttccgcc agttcgatcg
    17281 acgaaccgcc cgcttcctgg tttgaagcat ccagcgcgct ttttacttcg gcgagactga
    17341 tgccatattg cgccaggcgc tgaggatcga taaccacctg atactctttc accacaccgc
    17401 ctaccgacgc cacttccgca acgtcaggaa tggttttcag ctcatatttg agaaaccagt
    17461 cctgcaatga gcgtaaatcg gccagatcgt gcttaccgct gcgatccacc agtgcatatt
    17521 catagatcca gccaacaccc gtggcatctg gccccaactc ggcgctgact cccgcaggca
    17581 gcttaccctg tacctggttg aggtactcca gcacccgcga gcgcgcccag tacggatcgg
    17641 tgccatcttc gaaaatgaca tacacataag agtcgccaaa ctgcgaaaaa ccgcgcacag
    17701 tcttcgcgcc aggcaccgac aacatggtgg tggttagcgg ataagtcacc tgattttcaa
    17761 cgatttgcgg tgcctgaccg ggatagctgg ttttaataat cacctgcaca tcggagagat
    17821 ccggcagcgc atccactggc gtattaatga tggtccaggt gccccagata ctcagaaata
    17881 acgcgcccat cagcaccaga aaacggttcg ccaccgagcg acgaataatc cattcaatca
    17941 ttggttattc cctcaatgtg catgggtagc actttcagag cgcatccgct ccagtgcgcc
    18001 agaaatattg gcttcggaat caatcaggaa caggccgctg gaaaccacct tttcaccttc
    18061 cgccagacca gagcgtaacg cggtgacgcc ttgcgatgcc tggaaaacgg caacgcgttt
    18121 cggtacaaag cgcccgtcgg catcaacggt aatcacccgc tgttcgttgc cggtatcaat
    18181 cagcgcctgt gacggaatga gcagcatcgg ttcgctggcg gtgttgagtt gcaaccaggc
    18241 gttcattccc ggttttagcg cctcgtcggc gttgtcgact tccagacgca gctgcaacgt
    18301 gcgggtcgcg gcatccacgc caggtagtag cgtccatttg cggatggtga gtgttttatc
    18361 aggtcgcgcc ggaacggtga gcgtaaactg cgaggcatct ttcaccagcc aggcgatgga
    18421 ctccgggatc gcagcagtga cccacaccgg gtccataccc tgaattttcg cgaccacgtt
    18481 atctttggcg atattcattc ccgcgcgcag atcaaacgcg gtgatcacgc catcaatggg
    18541 cgctttgagc gtaaagcgag tctgtatttt ttgcgtggcg atcaggcggc gaatatccgc
    18601 ctccggcatt cccgccagtc tcagccgctc aagaatgcct tcagtctggg tcgccgtacc
    18661 gccggtttcg cgcagcagta aatactcact ctgcgcttcc acccagtcgg gaatggtcag
    18721 gtcgagaagc ggtgtgccct tttgtacttt atcgcccacg gtaagcggat acaccttgtc
    18781 gataaacccg gcagcgcgag cctgcacaat ggcatactga tactcgttgt aactgacatt
    18841 cgccgggaaa ctctgggcaa aagtcagcgg tccgcgcgtg acggtagccg ttttcacccc
    18901 cagattctgc gtctgagtcg ggtcaatgcg cacaccagac gcagaactct cttcatcggc
    18961 atatttcggc accagatcca tatccataaa cggcgattta cctggtttat cgaaccgcgt
    19021 attgggatac atcgggtcgt accagaataa gattttacgt tctgcggtcg acgttttttc
    19081 tgcgggcggt tcagcccggt caaaccaggt aaaacctgcc gcagaaataa taccgcccgc
    19141 gatcatgctg ccgataataa gcgcgatttt tttcatctca ttaaacctgg gttactggct
    19201 gactttaata tcctgtaata aagaaaggtt gccctgctgg acaaaattaa acgccacttt
    19261 gtcgccagtt ttaatttcac tcattttcgt ctgcggagtg atggtaaagc gcatggtcat
    19321 ctccggccag ttcaccgcgg taattggatc gtggtggatg gtgatttttt tgctttccag
    19381 atcaatggtt tttaccaccc cagtggcgct aatgacctgt ggttgtgcct cgctcatggt
    19441 ttcatgatga tgttcgttag cctgggcatt aaagccaatc acggtaaaca gactgaacat
    19501 tgcgacttgc agtgcttttt tcatttattc tctcctggag ttaaaaatta ttgctgccaa
    19561 ccgccaccaa gtgcggtata taaagaaatt tcgttgacct gacgggcata attcaggtca
    19621 agtaaggttt gtcgggttgc aaataaagaa cgctcggcat ccagcacttc cagataactg
    19681 actgcgccgt gctgatataa tgcccgcgcc cgttgcaaag taatttgcag cgacgccaga
    19741 taacgctgct gggcgctgat ttgatcgttc aggctttgac gtaatgccag cgcatctgcc
    19801 acttctttaa aggcgttctg gattttctgt tcataattca ctaccgactg ctgctggcga
    19861 atttcggcga tatccagatt ggcctggttg cgtccggcat taaaaatggg gatctcaatt
    19921 ttaggaataa aattccacat cccgctgctg gcgttaaata atgacgacag atcgctgctg
    19981 gcggttgata ttccgctggt caggcttata gatgggaaaa atgccgcgcg tgcggcccca
    20041 atattggcat tagccgccat taacgcgtgt tcagcttcca taatatccgg gcgctgcaat
    20101 aagatttgcg acgacaagcc cgccggtaat ttaacgcttt gcaggctgtc gctgtttact
    20161 gtctgcgctt gcggcagctt gccgtagctt cccaataata gttgcaatgc attattcgcc
    20221 tgcgccagtt ccccctgacg tttagcgatg tcgctgcggg tactttcaat caccccgcga
    20281 gcctgttcca gcgccagaac attgctgcta ccggtcaaca gttgtttttc gacaaacgca
    20341 tatgactgct gataattacg cagcgtttct tcggctattt gcaattgcgc atacgccagc
    20401 tgctgattga aatagctttg cgcgacatta gaaaccagca gaatatgcac cgcgcgctgg
    20461 gcttcctcag tggctaaata attttgtcgc tcggcttcgc tcacgttctt taagcgaccg
    20521 aaaaaatcga gatcaaagct ggcgttaagg ccagtcgaga actcccgcgt cgtggctgaa
    20581 tcgcctttaa gattgccgct ccagctgccg ctgccctcgc cattgagctg tgggtagcgg
    20641 tcggcatcgg tcagacgata ttgcgcccgc gcttcctgca ctttcagcgt cgccatgcgc
    20701 aaatcccggt tattcaccag cgcctcgcta atcagcgtct tcacctgatt atcaacaaaa
    20761 aaggtgcgcc agcccgcgtt ctggtagtta tctgctgcgt taaccaggcc gttctggttg
    20821 agtgagaact gctgcggcac gggcattgcc gggcgctgat aatccggtgc cagtgaacaa
    20881 ccggttagcg caagggccac acaaaatggc agaagtttac aaggagacat aggctcataa
    20941 tttctggtga ttttatgccg ccaactttac tcgccaggct ctgattttcc ggtgacagga
    21001 aaatgacaaa attgtcattt tgccaataag cgattgccat ctgatcccgc tactctagaa
    21061 ttgcccgggc aacatgcgga ggaaatatga aactgttgat tgtcgaagat gaaaagaaaa
    21121 ccggagaata cttgaccaaa gggttaaccg aagccggttt tgtggtcgat ttggccgaca
    21181 atgggctgaa tggctaccat ctggcgatga ccggtgatta tgacctgata atcctcgata
    21241 ttatgctgcc ggacgtgaac ggctgggata tcgtgcgcat gttgcgctcc gccaataaag
    21301 ggatgccgat tctgttgctt accgcgcttg gtaccattga acatcgcgtc aaggggctgg
    21361 agttgggggc ggatgactac ctggtgaagc cgttcgcttt tgctgaactg ctggcgcggg
    21421 tgcgcactct actgcggcgc ggggcggcgg taattatcga aagtcagttt caggttgccg
    21481 atttgatggt cgatctcgtc agccgcaaag tcacccgtag cggcacgcgc atcacgttga
    21541 ccagtaaaga atttactttg ctggagtttt tcctccgcca tcaaggcgaa gtgctgcccc
    21601 gctcgcttat cgcctcgcag gtgtgggaca tgaatttcga cagcgatacc aacgccattg
    21661 atgtggcagt gaagcggctg cgcggcaaaa tcgacaacga ctttgagccg aagctgattc
    21721 aaaccgtgcg cggcgtgggt tacatgcttg aggtgccgga tggtcagtaa gccatttcag
    21781 cgcccgtttt cgctggcaac ccgcctgacc ttttttatca gcctggccac catcgcggcg
    21841 tttttcgcct ttgcctggat catgattcac tcggtaaaag tgcattttgc tgagcaggat
    21901 attaatgatt taaaagagat tagcgccaca ctcaagcgcg ttcttaatca tcccgaagag
    21961 acacaagccc gacgcttaat gacgctggaa gatatcgtca gtggttattc caatgtgttg
    22021 atttccctgg cagatagcca gggtaaaacg gtgtatcact cccccggtgc gccggatatc
    22081 cgcgagttta cgcgtgacgc catacccgat aaagacacgc ggggcggcga ggtgtatctc
    22141 ctttccggcc cgacgattat gatgccgggc cacggtcacg ggcatatgga acacagcaac
    22201 tggcggatga ttaacttgcc ggttggcccg ttggtggacg gcaaaccgat ttatacgctc
    22261 tacatcgcgc tttcgatcga ttttcatctt cattacataa atgatttgat gaataaactt
    22321 attatgaccg catcgatcat cagcatcctg atcgtcttta tcgtgctgtt ggcggtacat
    22381 aaaggtcacg caccgatccg cagcgtcagc cgtcaaatcc agaacattac ctcgaaagat
    22441 ctcgacgttc gccttgaccc gcagaccgtg ccgattgagc tggaacagct ggtactgtcg
    22501 ttcaaccata tgatcgagcg tattgaggat gtctttactc gccagtccaa tttctcagcg
    22561 gatatcgctc acgaaattcg tacaccaatt acgaatctca taacgcaaac cgaaatcgcc
    22621 ctcagccagt cacgcagcca gaaggagctg gaagatgtgc tctactctaa tctcgaagag
    22681 ctgacgcgaa tggcgaaaat ggtcagcgat atgctgtttc tcgcccaggc cgataacaac
    22741 cagctaatcc ccgaaaagaa aatgctcaac ctggcggatg aaatcggcaa agtgttcgat
    22801 tttttcgagg cgctagcgga agatcgcggc gtggaactgc ggtttgttgg tgacgagtgt
    22861 caggttgcgg gcgatccgct gatgctgcgt cgggcgttaa gcaacctgct ttcgaacgcc
    22921 ctgcgttata cgccgcccgg agaggcgatt gtagtgcgct gccagacggt cgatcaccag
    22981 gtgcaagttt ccgtcgaaaa ccccggtacg cccattgcgc ccgagcactt accgcgattg
    23041 tttgaccgtt tctaccgcgt tgacccctcc cgccagcgaa aaggcgaagg tagcggtatt
    23101 gggctggcga tagtgaaatc gattgttgtc gcgcataaag gcacggttgc agtaacgtca
    23161 gatgctcggg ggacaaggtt tgtgattatg ctgccggaga gagagtgatt attaagttca
    23221 aattcagggc gtggtgcatc acctcctggt attgttgatc aacaaattag agaaaaactt
    23281 attaaacata tgaaatatcg aaggctttta tttttcaaag tttcacgaga tgtttgtata
    23341 tgaaacttag ttaaatctgt gtttcttcca attgtagatc acaatgccct ttctaaattt
    23401 tcatcgttat aaacaagcca tctatcatat aaagcaatga ataatttaat cgcgattaat
    23461 catcattaat tgttatatta ttcactcaat ctatttatca agtgaatttg ataaattcct
    23521 aatttaaaat atccactatt gagccagtat ttaacaacgt taagatgtgc aatgtgcaga
    23581 tcatgttatc agggtggagc ggtagcatat atttcttatg caatcattca ttttaataat
    23641 gttaattgga ttatttattg ctgtgaataa aaatatatta tgaatattgg tttaactttg
    23701 cgtttttgtt actcgttgtg atatgcgtac ctgtcctgaa taacgccaag aaacaagagg
    23761 ttgagcttgt gattattttt ttgactgaac agacacccgt ttctgtaaag taatatttaa
    23821 acaaaattct cacataacca tagcattttc tgttccttcc ctaataacgt ctgaagtcgt
    23881 cccgcttatc acattatccg cagttgtacg gtacggatca ttattcattt tactggaggt
    23941 tgctatgtca accggattac gtttcacact ggaagtggac ggcctgccgc cggatgcttt
    24001 tgcggtggtt tcctttcatc tgaaccagtc actctcttcg ctcttttccc tcgatctctc
    24061 cctggtcagc cagcagtttc tctcccttga atttgcgcag gtgctggaca aaatggccta
    24121 cctgacggta tggcagggcg atgacgtaca gcgccgggtg aaaggcgtgg tgacctggtt
    24181 tgaactcggg gagaacgaca aaaaccagat gctgtacagc atgaaggtgc acccgccgct
    24241 gtggcgtgcc ggtctgcgcc agaacttccg tatcttccag aacgaggaca tcaaaagcat
    24301 cctcggcacg atgttgcagg aaaacggggt gaccgaatgg agtccgctgt tcagcgagcc
    24361 gcatccttcc cgtgagtttt gtgtccagta cggtgagact gattacgatt tcctgtgccg
    24421 gatggcggcg gaggaaggca tcttctttta tgaggagcat gcttacaaaa gtaccgacca
    24481 gagcctggtg ctgtgcgaca cagtccgcca tctgcccgaa tcttttgaaa tcccatggaa
    24541 cccgaacacc cgtaccgagg tgagcaccct ctgcatcagc cagttccgct acagcgcaca
    24601 aatccgccct tcttccgtgg tgaccaaaga ctacaccttt aaacgccccg gctgggccgg
    24661 acgttttgaa caggaaggcc agcaccagga ctaccagcgc acgcagtatg aagtgtatga
    24721 ctaccccgga cgtttcaagg gtgcccacgg gcagaacttt gcccgctggc agatggacgg
    24781 ctggcgaaac aatgcagaaa ccgcgcgggg aatgagtcgc tcgccggaga tatggccggg
    24841 acgacgaatt gtgctgacgg ggcatccgca ggcgaacctg aaccgggaat ggcaggtggt
    24901 ggcaagtgaa ctgcacggcg aacagccaca ggcggtgcca ggacggcagg gagcggggac
    24961 ggcgctggag aaccattttg cggtgatccc ggcagacaga acatggcgac cacagccgtt
    25021 gctgaaaccg ctggtcgacg gcccgcagag cgctgtcgtg acaggaccgg caggcgagga
    25081 aatcttctgc gacgaacatg gtcgcgtgcg ggtgaagttc aactgggacc gctataaccc
    25141 gtcaaaccag gacagttcgt gctggatccg tgtggcacag gcgtgggcag gcaccggttt
    25201 tggccacctg gcgataccgc gtgtgggtca ggaggtgatt gtagacttcc tcaacggcga
    25261 tccggaccag ccgatcatta tggggcgcac ctaccaccag gaaaaccgca cccccggcag
    25321 cctgccggga acaaagacgc agatgaccat ccgctccaaa acgtacatgg gcagcggatt
    25381 taatgagctg aagtttgatg atgcgacagg gagagaacag gtctacatcc acgcgcagaa
    25441 gaacatggat accgaagtgc tcaacgaccg taccaccacc gtaaaacacg atcaccacga
    25501 aaccgtaaaa aatgaccaga cggtcacgat ccaggaaggt aaccgccttc ttacggtgga
    25561 aaaaggccac aagatcaccg gagtactgaa agggtcttta tctgaggatg tctttcagga
    25621 cagaggcacg attgccggtt cggtgcatgt tgacgctgta aacaatggtg gcgaaggcga
    25681 cggtatacag gcttatacgg cgattaagga aattttgctg gccgtggagg aaagcaaaat
    25741 tgcgctgacg ccggatggca ttcagctaca ggtcggggaa tcgacggtaa tcaggctgtc
    25801 gaaggatggc atcaccatcg tgggcggttc tgttttcatc aactgattca ctcagattat
    25861 cagggagtat tttatgcagt tcacttttaa tgaaggccac attcaactgc cgtctcaatg
    25921 gcaggatcag tcaatgcagg tcctggtgtc cacagataac agtggcatca acctggtcat
    25981 tacccgcgaa gccgttccac agggaaccct gacaccagag ctgtaccagg aaacgctggc
    26041 gctgtaccag ggcaaacttg atgggtacac cgaacatgcc tgccgggaaa tcactctggc
    26101 agaggccccg gcctggctgc tggattattc ctggaacggt ccggaagatg aggggaatca
    26161 ggggcggatt agccagatag ccgtattcca gcggcgtgga gatacgctgc tgaccttcac
    26221 tttcagcacc tccttatccc tgaagaacag tcaaaagacg atgctgctgg aggtgataaa
    26281 gagcttcacc ccgttgccac cggagaatga catccaaaag gaccagccgc gctgacataa
    26341 acgagggata acacatgagt gaaggaccag gcgggccaca gggagcgacc gcaggcggta
    26401 cgctggcaat gcgaatgctg tcacagcagg cgatggtcgc cagccagatg aaacgggcag
    26461 ccaacgacaa agccattgca cagatgctgg catcaaaaaa gtccggcccc cccgccgcca
    26521 ggctgggcga tgaaattcag cacaagagtt ttttgggggc gctggcaggg gccgtgctgg
    26581 gggcgatagt gaccatcgca gaaggttgcc tgattatggc cgcctgtgcc accggccctt
    26641 atgcgctggt tctggtgcct gcgctgatgt atgccagcta taaggcgagt gattatgtgg
    26701 aggagaaaca gaaccagctt gaatcatgga taaacagctt ttgtgacacg gacggcgcca
    26761 tcaataccgg ttctgaaaat gtaaacatta acggaaagcc cgctgccagg gccgccgtca
    26821 cccttccccc tcctccccca cctggagcaa tacctgaaat cccacagggg gaaccctcat
    26881 ggggtgatat tgccactgac ctgcttgaat cggcagcgga aaaagcagta ccactggcga
    26941 aggcctgggg gaacgctgtt atcaccctga cggacagcaa tgccggtttt atggatcgcg
    27001 tcagcgccgg cgcatcgctt ctgtttcccg ccggtccggt attaatggag tttgccacca
    27061 tggtgggcgg gcgtggcgaa atcaaaaaag atgtggattt cccggaagcc ggtgaggaca
    27121 cggcgctctg cgacaaggag aacaaaccac cgaggatagc ccagggcagc agcaacgtct
    27181 ttatcaacaa tcagcccgcc gcgcgcaagg gcgacaaact ggagtgcagc gcggcaatcg
    27241 tggaaggttc gccggacgtc tttattggcg gtgaacaggt gacctatctg gatatccagc
    27301 cagagttccc gccgtggcag agaatgatcc tggggggaat aacgatagcc agctatcttc
    27361 tgccgccagc gggactgctg ggaaaactga agaatctggc gagactcggc aaactgggga
    27421 acctgctggg gaaaagcggg aagctgctgg gggcaaagct cggcgcgttg ctgggaaaaa
    27481 caggtaagtc gttaaaaagt attgccaata aagtcatcag atgggtaaca gatcctgtcg
    27541 atccggtaac cggcgcgtac tgcgacgaac gtaccgactt tacgctgggc cagaccctcc
    27601 ccctctcctt cacccgtttc cacagttctg tactgccgct gcatggcctg accggcgtgg
    27661 gctggagcga ctcctggagc gaatacgcct gggtgcgtga acagggaaac cgggtggata
    27721 tcatcagcca gggagccacg ctgaacttcg ccttcgacgg tgacagtgat acggcggtta
    27781 acccgtatca cgcccagtac attctgcgcc gccgcgatga ttatctggag ctgttcgaca
    27841 gggatgcact gagcagccgc ttcttttatg acgcctttcc gggaatgcgt ctgcgccacc
    27901 cggtgactga cgataccagc gatgaccgcc tggcacacag ccccaatgac cggatgtaca
    27961 tgctgggcgg gatgagcgac accgccagca accgcatcac gtttgagcgc gacagccagt
    28021 accggatcac aggtgtcagt cacaccgacg ggatccggct taaactgacg taccacgcca
    28081 gcggctacct gaaagccatt caccgcacgg ataacggcat acagacgctg gcgacctacg
    28141 aacaggatgc gcgggggcgg ctgacagaag cggatgcgcg gctggactac cacctgtttt
    28201 atgagtacga cgctgcggac cggatcatcc gctggtccga taacgaccag acgtggagcc
    28261 gtttcaccta cgatgaacag ggccggtgcg tgaatgtcac cggggcggag ggctattaca
    28321 acgccacgct ggactatggt gacggctgca ccaccgtgac ggacggcaag ggcattcacc
    28381 gttattacta tgatcctgac ggcaatattc tgcgggaagc agcgccggac ggcagcacca
    28441 ccatgtatga atgggatgaa ttccatcacc tgctggcccg tcactcccct gccgggcggg
    28501 tggagaaatt tgaatacaac gccgcacacg gtcagttaag ccgttacacg gcggcagacg
    28561 gcgcggagtg gcagtaccgc tatgatgagc gcggcctgct cagcaacatc accgaccctg
    28621 ccgggcagac gtggacacag cagtgtgatg aacgcggcct gccggtaagt ctggtgtcgc
    28681 cacagggcga agagacccgg ctggcataca ccgctcaggg gctgctgtcg ggaatattcc
    28741 gccaggatga acggcgtctg ggcatagagt acgaccacca caaccggccg gaaacactca
    28801 ccgacgtgat gggccgcgaa caccacaccg aatacagtgg tcacgacctg ccggtgaaga
    28861 tgcgcggccc cggcggtcag tcagtgaggt tgcagtggca gcagcaccat aaactgagcg
    28921 gccttgagcg cactggcacc ggcgcggaag gattccgcta cgaccgtcac ggcaacctgc
    28981 tggcgtacac ggacggtaac ggtgttgtct ggacaatgga gtacggcccg tttgatttgc
    29041 cggtggcgcg aacggacggt gaaggccacc gctggcagta ccgctacgat aaagacacgc
    29101 tgcaactgac agaagtcatc aacccgcagg gtgagtcata tctttatgtt ctggataact
    29161 gcggccgggt gacggaagag cgtgactggg gcggcgtggt ctggcgttac cgttatgatg
    29221 ccgatggcct gtgtaccgcc agggttaacg gtctggagga aaccatcctc tacagccggg
    29281 acgccgcagg ccgcctggca gaagtcatca cgccggaagg caaaacgcag tatgcgtatg
    29341 acaaatccgg caggctgacg ggtatcttca gcccggacgg cacatcacag cgcaccggct
    29401 atgacgaacg cgggcgggtg aatgtcacca ctcagggccg acgggccatt gaataccact
    29461 accccgatga acacaccgtc atccgctgta tcctgccacc ggaagatgaa cgcgacagac
    29521 accccggtga atccctgctg aaaaccacgt accgctacaa cgcagccgga gaactggcag
    29581 aggtcatcct gccggaggat gagacgctga cgttcagccg tgatgaggca ggacgtgaag
    29641 tgctccggca cagtaaccgg ggttttgcct gtgagcaggg ctggaatgca gccggtcagc
    29701 ttaccagcca gcgcgccgga cttttcccgg cggaagccac atggggcgga ctgctcccct
    29761 cactggtgcg ggagtaccgt tacgacagcg cgggcaatgt gtcggctgtc accagccggg
    29821 aagattacgg acgggaaact cgccgggagt accggctgga ccggaacggc caggtcacgg
    29881 cggtgacagc ctcaggcacc gggctgggct atggcgaagg cgatgagtcc tatggctatg
    29941 acagctgcgg ctacctgaag gcgcagtctg cgggctggca ccggataagt gaagagactg
    30001 accagtatgc cggaggccac cggctgaaac aggccggaaa cacgcagtat gactatgacg
    30061 ccgcaggccg gatggtcagc cgcataaaac accgtgacgg ctaccgcccg gaaacagagc
    30121 ggttccggtg ggacagccgt gaccagctga ccgggtattg cagcgcacag ggtgaacagt
    30181 gggaataccg ccacgacgcc agcggcagac ggacggaaaa acgctgcgac cggaagaaaa
    30241 tccgcatcac gtacctgtgg gacggcgaca gtattgcgga aatccgggaa taccgcgatg
    30301 ataaactgta cagcgtacgg cacctggtgt ttaacggctt tgagctgata agccagcagt
    30361 gcagccgggt acggcagccg catccgtccg tggccccgca gtgggtgacg cggacgaatc
    30421 atgcggtgag cgacctgacg ggccgtccgc tgatgctctt taacagtgaa ggtaaaaccg
    30481 tctggcgacc ggggcagacc agcctgtggg ggctggcact cagcctgccc gcagacaccg
    30541 gctacccgga cccgcgcggg gaactggacg cggaagccga ccccggcctg ctgtatgcag
    30601 gacagtggca ggatgcggaa tccgggctgt gctataaccg gttccggtat tacgagccgg
    30661 aaacagggat gtacctggtg agtgatccgc tggggttgct ggggggtgag cagacttacc
    30721 ggtatgtgcc gaatccgtgt gggtatgttg atccgctggg attagcattt tgccccagaa
    30781 tgcagaagac attgcaaaaa ttaactgaca aggctgttgc tgatattatc gctaacccca
    30841 gactcgctga agatctgatg tcggcgggga gctatgcaca tttaaaaaat ggaactaatt
    30901 tatatgcagc cagttttggt aaggctgttg aacgtagggt tgcacagtta gttagtgaca
    30961 attcccgatt aagcaaactt gttgaacata ctggtcaggc caagggggct aatggacagt
    31021 ttatatcgag ccctgacttc accactaaag gtaaaggcgt gttcgacgtt acaacaaata
    31081 aagcactcca agcgcatata gacagatata ctaaagctgg tgtgtatact ccctataatg
    31141 atattggtta cttggtatat gatgtcgtat atggattatc tttctaaagc aaactaatgg
    31201 taactaaata tgacaaaagt tcttgagaat gaagagttca attctataca tatagacgaa
    31261 ggtggggttt ttaatagttt acgctttgaa aaatgtcttt ttcaaagctg tagcatact
//
LOCUS       DAOTRK010000049        30921 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.049, whole genome shotgun sequence.
ACCESSION   DAOTRK010000049 DAOTRK010000000
VERSION     DAOTRK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.049"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..977
                     /locus_tag="QIE41_002104"
     CDS             <1..977
                     /locus_tag="QIE41_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3530994.1"
                     /translation="DFNEKIGFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQLA
                     RRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKKL
                     LPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARVK
                     VALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNSS
                     ERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPEEDA
                     DDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1136..2329)
                     /locus_tag="QIE41_002105"
     CDS             complement(1136..2329)
                     /locus_tag="QIE41_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3530995.1"
                     /translation="MSPGIEDTPQKPLSCWSLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2465..4189
                     /gene="iucA"
                     /locus_tag="QIE41_002106"
     CDS             2465..4189
                     /gene="iucA"
                     /locus_tag="QIE41_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDS3530996.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4190..5137
                     /gene="iucB"
                     /locus_tag="QIE41_002107"
     CDS             4190..5137
                     /gene="iucB"
                     /locus_tag="QIE41_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDS3530997.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5137..6879
                     /gene="iucC"
                     /locus_tag="QIE41_002108"
     CDS             5137..6879
                     /gene="iucC"
                     /locus_tag="QIE41_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDS3530998.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6876..8213
                     /gene="iucD"
                     /locus_tag="QIE41_002109"
     CDS             6876..8213
                     /gene="iucD"
                     /locus_tag="QIE41_002109"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDS3530999.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            8219..10414
                     /gene="iutA"
                     /locus_tag="QIE41_002110"
     CDS             8219..10414
                     /gene="iutA"
                     /locus_tag="QIE41_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDS3531000.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10965..11108)
                     /locus_tag="QIE41_002111"
     CDS             complement(10965..11108)
                     /locus_tag="QIE41_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531001.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHRCVIQTVSFVRHILCYS
                     MGL"
     gene            11358..15245
                     /gene="sat"
                     /locus_tag="QIE41_002112"
     CDS             11358..15245
                     /gene="sat"
                     /locus_tag="QIE41_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HDS3531002.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYRVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGDVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSSANHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNVYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLDNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVNNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIISGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            15292..15873
                     /locus_tag="QIE41_002113"
     CDS             15292..15873
                     /locus_tag="QIE41_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531003.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILDHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYSIIKGKVSSVLLLLKMLQINHA"
     gene            16211..16645
                     /locus_tag="QIE41_002114"
     CDS             16211..16645
                     /locus_tag="QIE41_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDS3531004.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
     gene            16642..16992
                     /gene="tnpB"
                     /locus_tag="QIE41_002115"
     CDS             16642..16992
                     /gene="tnpB"
                     /locus_tag="QIE41_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDS3531005.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQIQHVLDDNPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            17023..18447
                     /locus_tag="QIE41_002116"
                     /pseudo
     CDS             17023..18447
                     /locus_tag="QIE41_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
     gene            complement(18701..19834)
                     /locus_tag="QIE41_002117"
     CDS             complement(18701..19834)
                     /locus_tag="QIE41_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HDS3531006.1"
                     /translation="MHSENIAAYVGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KHYAGKHWTGKHRTWLANQSFSQPSQQIAFQHYCQSLEQIEDRILQLDQEISRLLPEW
                     SLCNLVCQLQALKGVGQLIAITLVAELGDFSRFSNPKQLMAFLGLVPGEYSSGNSIRP
                     RGITKVGNSELRRLLYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSR
                     YRSLTAKGKKSQVAITAVARELTGFMWDIALAAQSSFSQQKQN"
     gene            19895..20083
                     /locus_tag="QIE41_002118"
                     /pseudo
     CDS             19895..20083
                     /locus_tag="QIE41_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(20114..20370)
                     /locus_tag="QIE41_002119"
                     /pseudo
     CDS             complement(20114..20370)
                     /locus_tag="QIE41_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            20462..21689
                     /locus_tag="QIE41_002120"
                     /pseudo
     CDS             20462..21689
                     /locus_tag="QIE41_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            22189..24279
                     /gene="iha"
                     /locus_tag="QIE41_002121"
     CDS             22189..24279
                     /gene="iha"
                     /locus_tag="QIE41_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HDS3531007.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPNLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(24497..24656)
                     /locus_tag="QIE41_002122"
                     /pseudo
     CDS             complement(24497..24656)
                     /locus_tag="QIE41_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            25141..25383
                     /locus_tag="QIE41_002123"
     CDS             25141..25383
                     /locus_tag="QIE41_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531008.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            25471..25662
                     /locus_tag="QIE41_002124"
     CDS             25471..25662
                     /locus_tag="QIE41_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531009.1"
                     /translation="MKKTKTHTGLLASKDKTRRVSLYETPTAWCIRGQECYSKSTGRR
                     CGSHDSLSRLRLDSIKPVE"
     gene            25674..26042
                     /locus_tag="QIE41_002125"
     CDS             25674..26042
                     /locus_tag="QIE41_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531010.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTEDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQYSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            26046..26261
                     /locus_tag="QIE41_002126"
     CDS             26046..26261
                     /locus_tag="QIE41_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531011.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(26814..27242)
                     /locus_tag="QIE41_002127"
     CDS             complement(26814..27242)
                     /locus_tag="QIE41_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HDS3531012.1"
                     /translation="MKKFINVYIKSDYDIVILRLSVIIIFMLFGTYKWFDFEVMALKP
                     LISPTWLNILYIMFGVHGGSYFLGIVETITFLALSIGFFRPAAGILGDIIVIVTGLVT
                     LSLLPQLGKVDSFVIKDVLLLGAGSVLLKYDLKNHLNIKN"
     gene            complement(27282..27842)
                     /locus_tag="QIE41_002128"
     CDS             complement(27282..27842)
                     /locus_tag="QIE41_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDS3531013.1"
                     /translation="MSTYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFP
                     RKTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLS
                     DSHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGAL
                     MLNRLPPHDTYSGTVNALIKMLDTRT"
     gene            complement(27884..28144)
                     /locus_tag="QIE41_002129"
     CDS             complement(27884..28144)
                     /locus_tag="QIE41_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDS3531014.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKLDGTAIIYR"
     gene            28693..29442
                     /locus_tag="QIE41_002130"
                     /pseudo
     CDS             28693..29442
                     /locus_tag="QIE41_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            29978..30091
                     /locus_tag="QIE41_002131"
     CDS             29978..30091
                     /locus_tag="QIE41_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDS3531015.1"
                     /translation="MKMKHLRDDVAAGRRRLYIRAICPSLREAPLLYSYPL"
     gene            30182..30921
                     /locus_tag="QIE41_002132"
                     /pseudo
     CDS             30182..30921
                     /locus_tag="QIE41_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    30463..30579
                     /locus_tag="QIE41_002132"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         8252 a         7276 c         7753 g         7640 t
ORIGIN      
        1 atgattttaa tgaaaaaata ggtttctggt atgtgatagc cctcgcagga cagtctaatg
       61 gtatggccta cggtgaaggt attcccctgc cagatacact cgacaaaccc gagtcccgtg
      121 taaaacaact ggctcgtcga aaaacaatca caccaggagg aaaagagtgt aaattcaatg
      181 agattattcc ggcagatcac tgcctgcatg atgttcagga tatgagtgga tatcaccatc
      241 cggctgctga tttgcataaa ggggaatatg gttgtgtcgg acagggattg catatagcta
      301 aaaagttact gccatacata cctgagcaag cagggatatt actggttcct tgttgcagag
      361 gaggtgccgc ctttactgtt ggagctgaag gtatgtatgt acctgataca ggggcaacag
      421 ctgatgcaat gcggtggggc accggaacag cactctatga ggacttagta gcccgggtta
      481 aggtagctct ggaatataac agaaagaata aactgctatc tgtctgctgg atgcaggggg
      541 agtttgatct tatgtcacca gattatgaaa agcatcctga cttgttttat cagatggtaa
      601 cttctttcag gtctgagtta tctgaatact catctcagtg tgttggtaat tcgtcagaaa
      661 gagttccatg gttgtgtggt gatacaacct ggtactggaa ggaatcttat caaaaagaat
      721 atgattttat atatggacat tacagacaac ggactgatga tgaaatacat tttctcagct
      781 ttcaggatag taaccgacat gaattaacta atgagccaga agaagatgcc gatgatttat
      841 ctgtcgggta tctggggtca tcatggagga cagaattaag ctggacaacg tcacagagaa
      901 gtacacattt taattcaatg gccagaaggg gagttattgc tgaatgttat gcgcaaaaaa
      961 taagaaatta tttgtaaaca agttcaggat atatctacag ctctgacatg attctctgag
     1021 agacatgacg gagtggcggg ggcagcaagc cagaacactg cctgacatgc tttcatcaga
     1081 cgcaggtcct ctctcatatc ctctgattta ctccagtaag gtgatgatca tatctttatt
     1141 tgatagtacg gtaatgccag ataaatacag acacaatggt aaataatata gttgcaaata
     1201 atgctgccat aatcagcgcg attgcgacac ctgttttcga taccagcggc accagcaaag
     1261 cgcacagtcc atagccaagt gtgtgactgg tagcgagcca gcccgcgcct gcgccatcag
     1321 ccagcttatc attcagtaga agttgataag cgggtgttgc cagagctgct ccaaatgaca
     1381 gcactgcaca gcccgggtaa aacaaccata gctgttcgga gagcatgata gccagaccac
     1441 cactcatcaa cactccggca ctcaggagca gggccaccgg agtcaggcgc tgcggacgga
     1501 gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag acccaacagc caggccacct
     1561 gttggctaat ggcggtggta tccgtggcga actggcgagt aagggcaggc gaaagtccaa
     1621 gctgcatcat gctcaccgcc gcagccagta atattgcgca aagcagataa ggcagacaat
     1681 cccgcttcag actgacgctc ttgcactccg gtgtgggctg tggtggtgtg ccgggcaacc
     1741 gcagcagcat cagcagcgcc agcactgggg ctgccatcag cagccccagt ggcgccagag
     1801 gatgaatggc cagcattgct gccgcgcaca gcggcccgaa tagcctgccg caactcaggc
     1861 cggagctgat ggttgccaga gcggccatgc gattcccttc tccagctctc tgcaatgccc
     1921 agacctgaca ggctggcacc attgccgaca cggtcagccc gtaggcgatc cgggcgatga
     1981 ttaatattcc cagtcccacc gctgttgtta tggcgctggt agccatcagc acgcttccca
     2041 gccctagcag ggtaaagctt atgccatatc ctcccagagc ccacaacacc acaggtttac
     2101 tgccagtgcg ggaaatttgc tttccccacc acggggacga tggcagaaac agcattgagc
     2161 caagcatcag cagggcagcc catacagaca gactcagatt tgtctgtatg accagaacag
     2221 ggagcacaac cagcagaccg ttctgcccga taccgagaag cccggcactg aacgcaagtg
     2281 accagcagga cagtggtttt tggggcgtat cttcgatccc aggtgacatt ttaatgtttc
     2341 aactccatgt attaattgtg tttatttgta aaattaattt atctgacaat aacatttctt
     2401 attgataatg agaatcatta ttgacataat tgttattatt ttactgtgtg ggagctgttt
     2461 gactatgacc ctgccctctg aaaaaccagc cacagatgtg gctgcgcagt gcttcctgaa
     2521 tgcactgatt cgtgaaacca cagactggaa actgacagaa tacccgccag acgaattgat
     2581 catcccgctg gatgagcaga aatcgctcca tttcagagtg gcttatttct ccccaaccca
     2641 gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca tcaggcagtt atcctgtcga
     2701 ctttaccact ctctcccggc ttattattga taagctacgg catcaactgt ttctgcccgt
     2761 tcccctctgc gaaactttcc accagcgcgt gctggaaagc cacgcccata cgcaacaggc
     2821 aattgatgcc cgtcatgact ggaccgccct gcgtgaaaaa gcgttgaatt ttggcgaagc
     2881 tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg cctaagtctc atgaaccgtt
     2941 taaccggcgg gaggctgaac gctacctgcc tgatatggca ccccacttcc cactgcggtg
     3001 gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg catctcaacc ttcaacagcg
     3061 gctgacgcga tttgccgcag agaatgcgcc tcagttactc aacgaattaa gtgacaacca
     3121 atggctgttc ccgttgcacc cgtggcaggg ggaatatctt ttgcagcagg ggtggtgcca
     3181 ggcacttgtt gctaaagggc tgattaaaga cttaggtgag gccggcacgt cgtggctgcc
     3241 gaccacctct tcccgttccc tctactgtgc caccagccgc gatatgatca agttctccct
     3301 gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg aaagaagtga agcgtgggat
     3361 gcgcctggca cgcctggctc aaaccgacgg ctggcagatg ctacaggtcc gcttcccgac
     3421 tttccgggta atgcaggagg atggctgggc cgggctgctc gatcttaacg gcaacatcat
     3481 gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg gatcagccga aaagccagac
     3541 caacgtactg gtctccctga ctcaggccgc acctgatggc ggtgattcgc tgctggtttc
     3601 ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg caacaggccg cccatgcatg
     3661 ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt acggctgaag cggattacgg
     3721 cctggtgctg ctggcgcatc agcaaaatat tcttgtccag atgcttgggg atctgccggt
     3781 cggatttatt taccgtgact gtcagggcag cgcttttatg cctcacgcga cagactggct
     3841 cgattctatt ggcgaggcgc aggcggaaaa tattttcacc catgagcagt tgctgcgcta
     3901 tttcccttat tacctgctgg ttaactccac ttttgctgtg accgctgcgc tgggggctgc
     3961 cgggctggac agcgaatcga atctgatggc tcgtgtacga gcatcgctgg ctgaagtgcg
     4021 tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg gaaagtccgt actggaacgt
     4081 aaaaggtaac tttttctgtt atctgaacga tcataacgag aacaccattg ttgacccttc
     4141 ggtgatctat ttcgatttcg ctaacccgct gcaggctcag gaggtctgaa tgtctgaggc
     4201 aaacattatt cacagcagat atggactgcg ctgtgaaaaa ctcgacaagc ccctgaatct
     4261 tggctgggga ctggacaata gcgcggtgtt gcactgtccc ggggagctgc cgacagggtg
     4321 gctgtgcgat gcgctggatc agatatttat cgccgcacca caactgtcag cagtggctct
     4381 cccctgggcc gaatggtgtg aggagccaca ggcgctgacg ctttttgggc aggtaaaaag
     4441 cgacattatc catcgtaccg ccttctggca gttaccgtta tggttgagtt caccggcaaa
     4501 ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag atttatttcc cgcagcgccc
     4561 accccgtccg cagggtgagg tttaccgccg ttacgatcca cgtattcgca ggatgctgag
     4621 tttccgcatt gccgatcccg tttctgatgc agaacgtttc acccgctgga tgaatgatcc
     4681 gcgcgttgag tatttctggg agcaaagtgg ctcactggag gtacagaccg cctatctgga
     4741 gcgccagctc accggtaaac atgcattccc gctgattggg tgcttcgacg atcggccatt
     4801 cagctatttt gaaatctact gggcggcgga agaccgcatt ggtcgtcact attcgtggca
     4861 gccctttgac cgtggcctgc atctgctggt tggtgaacag caatggcgcg gggcccacta
     4921 tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg ctggatgagc cccgtacgca
     4981 gcgcacagta ctggagccac gcaccgataa ccagcgcctg ttccgccatc ttgagcctgc
     5041 gggataccgg acaattaaag agttcgactt cccacataag cgctcgcgca tggtgatggc
     5101 ggatcgtcat cacttcttca cggaggtcgg tctgtaatga atcacaagga ctgggatttt
     5161 gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg agtatgagca ggttttccac
     5221 gcggaatctc agggcgatga ccactactgc attaacctgc cgggagcaca atggcgcttt
     5281 atcgctgaac gtggtatctg gggctggctg tggattgatg cccaaacgtt gcgctgcacg
     5341 gacgaaccag tactggcgca gacgctgctg atgcagctaa agccggtgct gtcaatgagt
     5401 gatgccactg ttgcagagca tatgcaggat ttgtatgcca cgctactggg cgacctgcaa
     5461 ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga ttaatcttga tgccgatcgt
     5521 ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta ataaaggtcg ccgtggctgg
     5581 ggcaaagagg cgctggaacg atatgcgcca gagtatgcca acaccttcag actgcactgg
     5641 ctggcggtaa aacgtgaaca tatgatctgg cgctgcgaca acgatctgga tattcagcag
     5701 ttgttgactg ccgcaatgga tccgcaggag tttacacgct tcagtcaggt ctggcaggaa
     5761 aacgggctgg atcacaactg gctgccgctg ccggtacatc cgtggcagtg gcagcaaaaa
     5821 atcgccaccg acttcatcgc tgattttgcc gaaggcagga tggtgtctct cggcgagttt
     5881 ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga ccaacgccag ccgccgggga
     5941 gggctggata tcaagctgcc gctgaccatc tacaacacct catgctaccg ggggatccct
     6001 ggcagatata tcgctgccgg accactggct tcacgctggc tgcaacaggt ttttgcgacc
     6061 gacgccaccc tggtgcaaag cggcgcagtg atccttggtg aaccggctgc aggctatgtg
     6121 tcccatgaag gctatgccgc gcttgcccgg gctccctatc gctaccagga aatgcttggt
     6181 gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg atgaaagtcc ggttctgatg
     6241 gcaacactga tggagtgcga cgaaaacaat cagccgctgg caggcgcata tatcgaccgc
     6301 tccggactgg acgctgaaac ctggcttacg caattgttcc gggtggttgt ggttccgctg
     6361 tatcacctgc tttgccgcta cggtgtcgcg cttattgcac acggacaaaa tataacgctc
     6421 gccatgaaag agggggtacc acagcgtgtt ctgctgaaag acttccaggg cgatatgcgg
     6481 ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc aggaggtgcg tgatgtcaca
     6541 tcccgtctga gtgcggacta cttaatccat gatttgcaga cgggtcactt cgtgacagta
     6601 ctgcgtttta tttcgccact gatggttcgt cttggcgtac ctgaaaggcg attttatcaa
     6661 ctgctggcag cagtgttgag tgattacatg aacaaacatc cacaaatgtc agagcgtttt
     6721 gcgcttttct cactcttcag gccacaaatc attcgcgtgg tgctgaaccc ggtaaaactg
     6781 acctggccgg accaggatgg cggcagtcgc atgctgccga attaccttga gaatctgcaa
     6841 aatccgctgt ggctggtaac tcaggaatat gaatcatgaa aaaaagtgtc gattttattg
     6901 gtgtcgggac agggccattt aatctcagta ttgctgcatt gtcacatcag atcgaagaac
     6961 tgaactgtct cttctttgac gaacatcctc atttttcctg gcatccgggt atgctggtac
     7021 cggattgtca tatgcagact gtctttctga aagatctggt cagtgcagtt gcacctacaa
     7081 atccctacag ctttgttaac tatctggtga agcacaaaaa gttctatcgc ttccttacaa
     7141 gcagactacg tacagtatcc cgtgaagagt tttctgacta tctccgctgg gctgctgaag
     7201 atatgaataa cctgtatttc agtcataccg ttgaaaatat tgatttcgac aaaaaaagtc
     7261 gattgtttct ggttcagacc agtcggggag aatattttgc ccgcaatatc tgccttggta
     7321 caggaaaaca accttattta ccaccctgtg tgaagcatgt gacacaatcc tgtttccatg
     7381 ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa acggataact gtggttggtg
     7441 gaggacagag tggtgcagac ctgttcctta atgcattacg cggggaatgg ggagaagcgg
     7501 cggaaataaa ctgggtctca cggcgtaata attttaacgc actggatgag gctgcttttg
     7561 ctgatgagta ttttacacct gaatatattt caggcttctc cggactgaag gaagatattc
     7621 gccatcagtt actggatgag cagaaaatga catcggatgg catcactgcc gattctttac
     7681 tgaccattta tcgtgagttg taccaccgtt ttgaagttct gagaaaacca agaaatatcc
     7741 gtctgctacc cagccgctcg gtaacaactc tggaaagtag tggtccgggc tggaagttat
     7801 tgatggaaca tcatctggat cggggcagag agagcctgga aagtgatgtg gtgattttcg
     7861 ccacaggtta ccgttctgca ttgccacaaa tacttccctc actgatgccc ttgatcacca
     7921 tgcacgataa gaacaccttt aaagtgcgtg atgacttcac tctggaatgg agtggcccga
     7981 aagaaaataa catctttgcg gtcaacgcca gcatgcaaac tcatggcatc gccgaacccc
     8041 agctcagcct gatggcctgg agatctgcac gtattcttaa tcgcgtactg gggcgtgatt
     8101 tattcgatct cagtatgccg cccgcactga ttcagtggcg cagcggcagc cggaaaaaac
     8161 cgcagccgga ggctgctgct ttaactcact atacaacaaa tattcaggaa taacgatgat
     8221 gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt cttgtcgtgc tgtcaccagc
     8281 tgtcgcccag caaaacgatg ataatgagat catagtgtct gccagccgca gcaatcgaac
     8341 tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat gccgaactgg agcagcagat
     8401 tcagggcggt aaagagctga aagacgcact ggctcagtta atccccggcc ttgatgtcag
     8461 cagccagagc cgaaccaact acggtatgaa catgcgtggc cgcccgctgg ttgtcctgat
     8521 tgacggtgtg cgcctcaact cttcacgttc cgacagccga caactggact ctgtcgatcc
     8581 ttttaatatc gaccatattg aagtgatctc cggcgcgacg gccctgtacg gtggcgggag
     8641 taccggaggg ttgatcaaca tcgtgaccaa aaaaggccag ccggaaacca tgatggagtt
     8701 tgaggctggc acaaaaagtg gctttaacag cagtaaagat cacgatgagc gcattgccgg
     8761 tgctgtctcc ggcggaaatg accatatctc cggacgtctt tccgtggcat atcagaaatt
     8821 tggcggctgg tttgacggta acggcgatgc caccctgctt gataacaccc agaccggcct
     8881 gcagcactcc aatcggctgg acatcatggg aaccggtacg ctgaacatcg atgaatcccg
     8941 gcagcttcaa ctgataacgc agtactataa aagtcagggg gacgacaatt acgggcttaa
     9001 tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca ccatacgtca gtaaggggct
     9061 gaattctgac cgcattcccg gcactgagcg gcatttgatc agcctgcagt actctgacag
     9121 tgatttcctg agacaggaac tggtcggtca ggtttactac cgcgatgagt cgttgcggtt
     9181 ctacccgttc ccgacggtaa atgcgaataa acaggcgacg gctttctcct cgtcacagca
     9241 ggataccgac cagtacggca tgaaactgac tctgaacagc caacttatgg acggctggca
     9301 aatcacctgg gggctggatg ctgagcatga gcgctttacc tccaaccaga tgttcttcga
     9361 tctggctcag gcaagtgctt ccggagggct gaacaaccat aagatttaca ccaccgggcg
     9421 ctatccgtca tatgacatca ccaatctggc ggccttcctg caatccagct atgacattaa
     9481 tgatattttt accgttagcg gtggcgtacg ctatcagtat actgagaaca gggtagatga
     9541 tttcatcgac tacacgcagc aacagaagat tgctgccggg aaggcgatat ctgccgacgc
     9601 cattcctggt ggttcggtag attacgataa ctttctgttc aatgctggtc tgctgatgca
     9661 catcaccgaa cgtcagcagg catggttcaa tttttcccag ggggtggcat tgccggatcc
     9721 ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg aacggccatc ttcccctgac
     9781 aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg aaagtcgatt cttatgaact
     9841 gggctggcgc tttaccggtg acaacctgcg gactcaaatc gcggcatatt actcgctttc
     9901 caataagagc gtggaaagga ataaagatct gaccatcagt gtgaaggacg acaggcgccg
     9961 tatttacggc gtggaaggtg cggtggacta cctgatcccg gatactgact ggagtaccgg
    10021 tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac ggtcaatggc aaaaatatga
    10081 cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt aactgggcgc cggaaccgtg
    10141 gagtctgcgt gtacagagca ccacttcttt cgacgtaagc gatgcagagg gtaacgatat
    10201 taatggttac actaccgtcg attttatcag tagttggcag cttccggtgg gaacactcag
    10261 cttcagcgtt gagaacctct tcgaccgtga ctataccact gtctggggac agcgtgcacc
    10321 tctgtactac agcccgggtt acggccctgc ttcactgtac gactacaaag gccggggccg
    10381 aacctttggt ctgaactact cagtgctgtt ctgacgtgga tgcttttgca acaagggtat
    10441 gctgtaaaca acatggtcct tggtggccat gttgtttaca gccagtataa tatacagcct
    10501 taacaagata ttccgtctcc ggaatggact gttcaccact gaataacatt atgtctgttt
    10561 tatggatctt tgactgaaat ttatcgcata tcctcatttc attgtccgtc tgaaaactaa
    10621 ccttacaagt aagcgtcgtc tcagcactgt ctggcagatc ctgaaattcc tgagaggatg
    10681 atctgcgccc aatatcctga gtcggaactt tgctgcaatg aaggccaacg aaaaatgggg
    10741 gagtatgtta tagagtactt gctacagggt aaaaagaggt acctgtcgct ggttctccgt
    10801 tcagtttaac cgggagattt tctcctgcgc ctgtcataaa gtccggtaat ggagattgtt
    10861 aatacgatgg tgcacgatgc attgtcagtg cccggcccgg aggatgcccc gtagctacat
    10921 acggatcagg gttggcaata tcgaatggca ggctataggc aaagttaaag ccccatggag
    10981 tagcacaaaa tatgccgcac aaaggaaacg gtctgaataa cgcagcgatg gagaacttct
    11041 tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt cgtcgtgaat cactgaacga
    11101 tgttccatgc gaacagatat tcgatcatat cgaaatatta aacattaccc ctccccacat
    11161 tgggcaaaaa aaaacaaaaa acgatcaatt aacgatcaat tagtattgat tcacataatc
    11221 tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta cttttagcaa
    11281 gctgttagta accaaccgtt actaataatt ttaattctta atactttgtt cattcaataa
    11341 atgagttgag agaatatatg aataaaatat actcccttaa atatagtgct gccactggcg
    11401 gactcattgc tgtttctgaa ttagcgaaaa gagtttctgg taaaacaaac cgaaaacttg
    11461 tagcaacaat gttgtctctg gctgttgccg gtacagtaaa tgcagcaaat attgatatat
    11521 caaatgtatg ggcgagagac tatcttgatc ttgcacaaaa taaaggtatt ttccagcccg
    11581 gagcaacaga cgtaacaatc actttaaaaa acggagataa attctctttc cataatctct
    11641 caattccgga tttttctggt gcagcagcga gtggcgcagc taccgcaata ggaggttctt
    11701 atagtgttac tgttgcacat aacaaaaaga accctcaggc cgcagaaacc caggtttacg
    11761 ctcagtcttc ttacagggtt gttgacagaa gaaattccaa tgattttgag attcagaggt
    11821 taaataaatt tgttgtggaa acagtaggtg ccaccccggc agagaccaac cctacaacat
    11881 attctgatgc attagaacgc tacggtatag tcacttctga cggttcaaaa aaaatcatag
    11941 gttttcgtgc tggctctgga ggaacatcat ttattaatgg tgaatccaaa atctcaacaa
    12001 attcagcata tagccatgat ctgttaagtg ctagtctatt tgaggtcacc caatgggact
    12061 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
    12121 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
    12181 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
    12241 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
    12301 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
    12361 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga gatgtcctgt
    12421 ttaaggataa tttggatttt ggtagcggtg gtattatctt tgacgaaggc catgaatata
    12481 acataaacgg tcagggattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
    12541 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccctggta
    12601 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
    12661 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
    12721 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
    12781 gtggtacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
    12841 atggaacaac aataactaac tcagatacaa cgaaagaagc cgttctggca atcaataacg
    12901 aagactccta catatatcat gggaacataa atggcaatat aaaactaaca cacaatatta
    12961 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
    13021 atgatgttga agtcagtaat gccagtctta ccatgcaagg ccatgcaaca gagcatgcaa
    13081 tattcagaag ctcagcgaat cattgctccc tggtatttct ttgtggaacg gactgggtca
    13141 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
    13201 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
    13261 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
    13321 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgtttacatt gataatcttg
    13381 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
    13441 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
    13501 gtgatcaagc tgtagttaca cttaatggtg caacctttct ggataatact cctataagta
    13561 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
    13621 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
    13681 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
    13741 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
    13801 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
    13861 ttttgtatgg ctttgatacc gcttatcgag gcgcaataac agcccccaaa gctacagtta
    13921 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
    13981 aagacagtat ggtgcgtttt actggtgata atgggaagtt tacaaccctt acagtgaaca
    14041 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcagatcagc
    14101 ttgttgtcaa taaatcgttg tctggtaaaa acaaccttct gttagtcgac ttcattgaga
    14161 aaaatggaaa cagcaacgga ctgaatatcg atctggtcag cgcaccaaaa ggaactgcag
    14221 tagatgtctt taaagctacg actcggagta ttggcttcag tgatgtaaca ccggttatcg
    14281 agcaaaagaa cgatacagac aaagcaacat ggactctgat cggctataaa tctgtggcca
    14341 acgccgatgc ggctaaaaag gcaacattac tgatgtcagg cggctataaa gccttccttg
    14401 ctgaggtcaa caaccttaac aaacgtatgg gtgatctgcg tgacattaac ggtgagtccg
    14461 gtgcatgggc ccgaatcatt agcggaaccg ggtctgccgg cggtggattc agtgacaact
    14521 acacccacgt tcaggtcggt gcggataaca aacatgaact cgatggcctt gacctcttca
    14581 ccggggtgac catgacctat accgacagcc atgcaggcag tgatgccttc agtggtgaaa
    14641 cgaagtctgt gggtgccggt ctctatgcct ctgccatgtt tgagtccgga gcatatatcg
    14701 acctcatcgg taagtacgtt caccatgaca acgagtatac cgcaactttc gccggccttg
    14761 gcaccagaga ctacagctcc cactcctggt atgccggtgc ggaagtcggt taccgttacc
    14821 atgtaactga ctctgcatgg attgagccgc aggcggaact tgtttacggt gctgtatccg
    14881 ggaaacagtt ctcctggaag gaccagggaa tgaacctcac catgaaggat aaggacttta
    14941 atccgctgat tgggcgtacc ggtgttgatg tgggtaaatc cttctccggt aaggactgga
    15001 aagtcacagc ccgcgccggc cttggctacc agtttgacct gtttgccaac ggtgaaaccg
    15061 tactgcgtga tgcgtccggt gagaaacgta tcaaaggtga aaaagacggt cgtatgctca
    15121 tgaatgttgg tctcaacgcc gaaattcgcg ataatcttcg cttcggtctt gagtttgaga
    15181 aatcggcatt tggtaaatac aacgtggata acgcgatcaa cgccaacttc cgttactctt
    15241 tctgataaca gcccgggccg cgtttgcggc ccttcttcta ccggagagaa tatgtattac
    15301 cctgtgacag actatatcgc tcttgctctc attattagct ttctttttct gacattattt
    15361 atctgcctgt tatgccttaa acatgagcga ataaaaaaag aaactatcag gcaaaaaaat
    15421 gcacatattc tggatcatgg ctggaatgca actgagttct catggttccg atacggacag
    15481 tataacgaaa cgggtattta catctcgatc gagaagacca ttatcattac aatcaccgtt
    15541 tctggtgggt gctttaaaaa agaatacagc attgtctccc acatgctggt cactgacacc
    15601 ataacagagg ccaccctgta cgaaaacggg ctgtatacgc gccacatcag actcagtcgc
    15661 ccagtatctg acagcaaata ccccttgcct cccggatctc agctcattaa aaatatgacc
    15721 ttacgactgc gcctgcagga tcagcaggaa gaaacatccg tgacattatt tcagggaaaa
    15781 atgagtaccg acggaaataa ctattccatc atcaaaggta aagtaagctc agtacttctg
    15841 ttattaaaaa tgctgcagat taatcacgcc tgatctctgt cccggtattt tagcccgagg
    15901 tatattctga aatatcattc atcatgaccg gcgtttatac aaacgccggt cattcgctaa
    15961 cagcttcacc gttccttccc ccatccaggt tccgcatgag cgtgttgtaa tgttcataag
    16021 cgtattatca ggattgtcgt aagcgtaaac tgaccgccgt atgtagccat taagccagta
    16081 ttggtaacgt aggtgcccac cttttcatca actagtggac acctggtaag cgtaaagcca
    16141 atgccgtctg taacacctgc tccttgcaga ctaaattaga gctccttcta aattagacgg
    16201 agttctattg atggataaac ctacagactg gcgctccgga acccgccgga tattttctaa
    16261 tgaatttaaa cttcatatgg ttgaactggc ttcgaaacca aatgccaatg ttgcacaact
    16321 ggcccgggaa catggcgttg ataacaacct gatttttaaa tggctacgcc tctggcaaag
    16381 agaaggacgt atttctcgta gaatgcctcc aactattgta ggccctacag tatcacaatc
    16441 ttttccggcc tctccgactc tggttccagt ggaacttatc gacaccccgc gctgtgctac
    16501 agatgctcct gctccggagg cattatcagt tgcttgtgca gcttcctgcc atgtggaatt
    16561 ccattacggt aaaatgatgc tggaaaatcc ttcaccagag ctgctcacgg tgttgatccg
    16621 tgagctgacc gggaggggac gatgatttca ctcccatcag gtacccgtat ctggctcgtt
    16681 gccggcgtta ctgatatgcg taaatccttc aacggtctgg gggagcagat acagcatgtg
    16741 ctggatgata accccttctc cggtcacctg tttatcttcc gtggccgacg gggagacacg
    16801 attaaaatcc tctgggctga tgctgatggt ctgtgcctgt tcaccaaacg ccttgaggaa
    16861 ggtcagttta tctggcctgc ggtgcgtgac ggtaagatat ccattacccg ctcgcaactg
    16921 gcaatgctcc tcgataagct ggactggcgt cagccaaaaa catcccgcct taacgcactg
    16981 acaatgttgt aaaaaactcc tgaccgcatt ataaaaacgg ccatgagtca gaaatacctc
    17041 attcgcatcg ctgagctgga aaggctgctc tctgagcagg ctgaagccct ccgtcagaaa
    17101 gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc actggcacgt
    17161 gccgaagaaa agatcgaaga agatgaacgg gaaatagagc atctgcgggc tcagatagaa
    17221 aaactgcgcc ggatgctgtt cggaacccgt tctgaaaaac tgcgtcgtga ggttgaacag
    17281 gctgaagccc tgctgaaaca acgcgagcag gaaagcgatc gttacagtgg gcgtgaggat
    17341 gacccgctgg ttccccgcca gttgcgacag tcacgccatc gtcgtccgtt accggcatat
    17401 ctcccccgtg aaatataccg cctggagcct gaagaaagct gttgcccgga gtgtggcggt
    17461 gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag cagcgccctg
    17521 aaagtgatcc gcacagaacg ggtaaaaaaa gcctgtacaa aatgtgactg catcgttgaa
    17581 gcaccggcac catcccgtcc gatagagcgt ggtatcgcgg gcccggggtt acttgcccgc
    17641 gtgttaacgg gaaaatactg cgaacacctg ccactgtatc gtcagagtga aatctttgcc
    17701 cgccagggtg tcgaactgag ccgtgcctta ctctccaact gggttgatgc gtgctgccag
    17761 ttaatgactc cgctgaatga tgccctgtac agttatgtga tgaacacccg caaggttcac
    17821 actgatgaca caccagtaaa agtactggca ccgggcagga agaaggcgaa aacaggatat
    17881 atctggacgt atgtccggga tgaccggaat gccggttcgc cagaacctcc ggcggtctgg
    17941 ttcgcctact caccggacca tcagggtaaa catccggagc aacaccttcg tcccttccgg
    18001 ggtatcctgc aggcagatgc gttcgcaggt tacgatcggc tgttcagtgc cgaacgtgaa
    18061 ggcggcgcgt tgacggaagc aggatgctgg gctcatgcgc ggcgcaaaat ccacgatgta
    18121 tatatcagta ctaaaagcgc gacggcggaa gaagcactga aactaatcgg cgaactgtac
    18181 gccattgagc acgaaatacg cgggttgccg gtgtctgaac gcctggcggt caggcaaatg
    18241 cagagtaaac cgctactgac ttccctgtat aagctgatgc aggagaaaga acacacgtta
    18301 tcgaaaaaat gccgtctgag agatgcgttc cggtatatca ggaagcactg ggttgcgttg
    18361 tgcaacttct gtgatgacgg tctggcggag gcggacaata acacagcgga aagagcgctt
    18421 cgtgcagtct gtctcggaaa gaaaaattct tatgaccttt gtcaaatatt aagcccaaaa
    18481 agaccttacg cagctccggg agcttcgtta tatcgcggat taaagaacct gaaacagtca
    18541 gactgtatct tggattttac aaactcttat tcggggattt tagccccatg acgatttacg
    18601 cagtgagctg gcctctttct acgtgcaggt attagcgcat tctggcttcc ctgaattctt
    18661 ttacgaggat cactcattcc gtgtctgact gcttcagggt ttaattttgc ttctgctgac
    18721 tgaatgatga ttgggcagca agtgcaatat cccacataaa tccagtcaac tcacgagcca
    18781 ccgccgtaat ggctacttgt gattttttcc cttttgctgt cagacttcgg taacgagaac
    18841 acaatcgttg ttgagctttc catgcaatat ctttggaata ttgtgttaca gagtccggtc
    18901 ggtaatatat aagccatgcc ccaacttttg caggtgtacg ataagaccag gcggcttcgt
    18961 aaagcagacg tctcagttcg ctgtttccaa cttttgttat tcctctggga cgaatactgt
    19021 ttccactgga atattcacct ggcaccagtc ccagaaaagc catgagctgt ttgggattgg
    19081 aaaatcgcga aaaatcaccc agttcagcaa ccagagttat tgcaattagc tgaccaacac
    19141 ctttaagagc ctgcaactgg cagaccagat tgcacagaga ccactcaggt aacaggcgag
    19201 atatttcctg gtcgagttga agtatgcgat cttcaatctg ctccagactc tgacaatagt
    19261 gttgaaaagc aatctgttga gatggctgac taaatgattg attagctaac caggttcgat
    19321 gttttcctgt ccagtgcttt cctgcataat gtttgtctgt tcgcaatagc atgctcagaa
    19381 ttcgctgtcg ggcaactctg ctgtctcgtt tagctgctgc acgggctctg attagatccc
    19441 gcatagcttc atgagtcagg tcagggatcc agacaggggt aagttctcca gcccgcagta
    19501 acctggcgag agaaattgcg tcccgatgat catttttgat tctgtctgtc ggtgattttg
    19561 gtatgcggga aggggcaata acctggcatt ttatattcat cgctgtgagt tgatgatata
    19621 gcccaaaacc acaggggcca gcctcatagc aggaaagaat gtttccgtaa agtccctgca
    19681 atttctgaaa taatcgacgt acagcctggg cttcgttgtt aatcgtacca taataacgaa
    19741 cttcgccaag ccgttcaggt gcagcaatag caaccgccaa cgtttcttta tgcacatcca
    19801 gcccgacgta agccgcaata ttttccgaat gcatgatgac ctcctctgga atatcccacc
    19861 aatgctatag tgagtatata aaggtcatat catctacgtg ttcttcggta gcgatcacgg
    19921 cggcgagcgt ggtgcactgt tgtacgggct gatcggcacc tgccggttga atggtatcga
    19981 tccggaagcg tatctgcgcc atatcctgag cgtactgccg gaatggcctt ccaaccgagt
    20041 tgacgaactc ctgccatgga acgtagtact cactaataaa taagcgtcaa tacggtgctc
    20101 cgttgacgct tacacgcacc tgcttttcca gctcacgaat acattgctgc tctgatgtga
    20161 atggtgctgc agaaggcgtg atctcctgac gttcccgtct gagctgacat acccatgcct
    20221 ccagagtaga gaaaccaaca ttcatagctt cagtagcttc ccggtatgag taacctttat
    20281 caacgataag ttgggcgcat tccagattaa actcaggaga gaaggtacgc tttgttttct
    20341 tgttgtttta tgttgtggtg agaatatcac tagactggcc ccctgaatct ccagacaacc
    20401 agtatcactt aaataagtga tagtcttaat actagttttt agactagtca ttggagaaca
    20461 gatgattgat gtcttaggac cggagaaacg cagacggcgt actacacagg aaaagatcgc
    20521 tattgttcag cagagctttg aaccgggaat gacggtctcc cttgttgccc ggcaacatgg
    20581 tgtggcagcc agccagttat ttctctggcg caagcaatac caggagggaa gtcttagtgc
    20641 tgtggccgca ggagaacagg tcgttcctgc ctctgaactt gctgctgcca tgaagcagat
    20701 taaagaactc cagcgcctgc tcggtaaaaa acgatggaaa atgaactcct taaagaagcc
    20761 gttgaatatg ggcgtgcaaa aaagtggata gcgcacgtgc ccttattgcc cggggatggg
    20821 gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac gtcattctca
    20881 gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat acggatgtgc
    20941 ttctccgtat ataccatgtt atcggagagc tgcccacgta tggttatcgt cgggtatggg
    21001 cgctgcttcg cagacaggcc gaacttgatg gtatgcctgc gatcaatgcc aaacgtgttt
    21061 accggatcat gcgccagaat gcgctgttgc ttgagcgaaa aaccgctgta ccgccatcga
    21121 aacgggcaca tactggcaaa gtggccgtga aagaaagcaa tcagcgatgg tgctctgacg
    21181 ggttcgagtt ccgctgtgat aacggagaaa aactgcgagt cacgttcgcg ctggactgct
    21241 gtgaccgtga ggcactgcac tgggcggtca ctacgggcgg cttcgacagt gaaacagtac
    21301 aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa cgagcttccg gcgtctccag
    21361 tagagtggct gacggataat ggttcatgct accgggctaa tgaaacacgc cagtttgccc
    21421 ggatgttggg acttgaaccg aagaacacgg cggtgcggag tccggagagt aacggcatag
    21481 cagagagctt cgtgaaaacg ataaagcgtg actacataag tatcataccc aaaccagacg
    21541 ggttaacggc agcaaagaac cttgcagagg cgttcgagca ttataacgaa tggcatccgc
    21601 atagtgcgtt gggttatcgc tcgccacggg aatatctgcg gcagcgggcc tgtaatgggt
    21661 taagtgataa cagatgtctg gaaatatagg ggcaaatcca ttgtcagcgc caacaatgtg
    21721 ggctgacatg ataaagctgt ctaggaaatc gttcgcctcc tcagcggaca atccaaatgg
    21781 tgattgtctc tgttaaacgt ttattttgaa ggtcgactga ataaggtgat gacgctgtag
    21841 aatttttcac gtgccacaga attttgaacg ctttctctta caatatttca atgtttctat
    21901 cagtattcgc cggaagaagt catcgaccaa atcatcccag tcgtctcgca ccactgacca
    21961 ttcatggttg acatgtggtg gaaatccgct tctacagtaa ccatttttta ttcgcaaaac
    22021 cgaacacgcc atacggatag ctgttaactg gcatgccgag gcagtcgtta tttatattta
    22081 gttttgtcaa taatctttat tttttgtaaa aggtaaatat aaattattct cattattgtt
    22141 tgtatttgtg tattgtcttg ccggttaaca tgatcggaga ttagtaatat gcgaataacc
    22201 actctggctt ccgtagtcat tccctgtctc ggattttcag ccagcagcat agctgctgca
    22261 gaggatgtga tgattgtctc ggcatccggc tatgagaaaa agctgactaa cgcagccgcc
    22321 agtgtttctg tgattagcca ggaggaattg cagtccagcc agtaccacga tctggcggag
    22381 gctctgagat cagtagaggg tgtggatgtt gaaagtggta cgggtaaaac cggtgggctg
    22441 gaaatcagca tccgaggaat gccagccagt tacacgctga tactgattga tggtgttcgt
    22501 cagggcggaa gcagtgacgt gactcccaac ggtttttctg ccatgaatac cgggttcatg
    22561 ccccctctgg ccgccattga gcgtattgag gttatcaggg ggccgatgtc cacactgtat
    22621 ggctctgatg cgatgggcgg tgtggtgaat atcattacca gaaagaatgc agacaaatgg
    22681 ctctcttccg tcaatgcagg gctgaatctg caggaaagca acaaatgggg taacagcagc
    22741 cagtttaatt tctggagcag tggtcccctt gtggatgatt ctgtcagcct gcaggtacgc
    22801 ggtagcacac aacagcgtca gggctcatcg gtcacatcac tgagcgatac agcagccacg
    22861 cgtattcctt atcccacgga gtcacagaat tataatcttg gtgcacgtct tgactggaag
    22921 gcgtcggagc aggatgtgct ctggtttgat atggatacca cccggcagcg ttatgataac
    22981 cgggatgggc aactggggag tctgacgggg ggatatgacc ggaccctgcg ctatgagcga
    23041 aacaaaattt cagctggcta tgatcatact ttcaccttcg gaacatggaa atcgtatctg
    23101 aactggaacg agacagaaaa taaaggtcgt gagctcgtac gcagtgtact gaagcgcgac
    23161 aaatgggggc ttgccggtca gccgcgggag cttaaggaat cgaaccttat cctgaattca
    23221 ttactgctta cccctctggg agaatctcat ctggttacgg tggggggcga gtttcagagc
    23281 tcgtccatga aagacggagt tgtccttgcc agcacaggtg aaactttccg gcagaaaagc
    23341 tggtcggtat ttgctgagga tgagtggcat ctcacggatg cacttgcgct gactgcgggc
    23401 agccgctatg aacatcatga gcaattcggg ggacacttca gtccgcgtgc atatctggtc
    23461 tgggatgtgg cagatgcctg gacgctgaaa ggcggtgtga ccacgggata taaggcaccc
    23521 agaatggggc agctacataa agggattagt ggtgtatccg ggcagggaaa aacaaatcta
    23581 cttggtaacc ccaacctgaa gccggaagag agcgtcagtt atgaggctgg ggtgtattac
    23641 gataaccccg ccggtctgaa tgccaatgtc acaggtttta tgactgactt ctccaacaag
    23701 attgtctctt attccataaa tgataacacc aatagctatg taaacagcgg aaaggcccgg
    23761 ttgcatggtg tggaatttgc cggcacattg ccgctgtggt cagaggatgt cacgctgtca
    23821 ctgaattaca cctggacccg aagtgaacaa cgtgatggtg ataacaaagg tgcgccgctg
    23881 agttataccc ctgaacacat ggtgaatgcg aaactgaact ggcagatcac cgaagaggtg
    23941 gcatcatggc tgggtgcccg ttatcgcggg aaaacaccac gtttcaccca gaattattcg
    24001 tcactgagcg ctgtacagaa gaaagtgtat gatgagaaag gagaatacct gaaagcctgg
    24061 acggtggtgg atgcaggtct gtcgtggaag atgacggatg ccctgacgct gaatgctgcg
    24121 gtgaataacc tgctcaacaa ggattacagt gacgtgagcc tgtacagtgc cggtaagagt
    24181 acgctgtatg ccggtgatta cttccagacg ggatcatcaa caacaggata tgtgatacct
    24241 gagcgaaatt actggatgtc gctgaactat cagttctgat aataacaaaa cgctatcact
    24301 gacggtagaa tacgttgcca ctgcaactcc tggcggaaca gtggcaacgt attaggttaa
    24361 gtgcatttcc gatccgctaa tgagatttcg ttaccaacaa ctaatatcgt cacaggaaat
    24421 gcacggatta tttttaactt atcatttaca tacttgtcca gagtgtaagc gcaccgtgaa
    24481 ggacgtgggg taaaaattag tttacagaga gagtgacgtt ccagggcaac aactctttca
    24541 tgcggttggc aggccaggtg ttgattacac tgatcacgtg gcgtagccac gtttccggct
    24601 cgattccgtt aagttttgca gctaccgatc aggctgtaca tcactgccgc actatcgctc
    24661 gtcatctcaa agtccggtct cgtcagcagg aaggtatcat tctctcccgc catttttccg
    24721 ggggcccggt cagataagtc cctttgtcta tcgctgactc ctggctcata acccggttag
    24781 cagaatgcag ggtcaccacc tcgccacgac cgaatccgga taagtcaatt gcaccttctc
    24841 aatcgccatt ttgtcacaat taagtttgat aaagcaggtc acctctgcac tgaatctgct
    24901 ccagtgcctc attggtctcc gtccacccct ttaaactgcc ggataagact gctgcacagc
    24961 gccttccccg gaatcaaatc actttccctg tccgccacaa tccgcttcat gccacctcgt
    25021 attttgtttc agagaccgga atctgccaga aagtgactac gcgtgcacat atcggttttc
    25081 tgggttacat gcatcgtgtt attttctgaa atgcaaaata ataaaaaaca aggacacatc
    25141 atgaaggatg aaatacacag taccagcatt aacccggatg ttgttgagca gtttctggcc
    25201 cttatgaaca cacattgtaa atcctcagaa gaggcactcg ccatgctgag tacgctttgt
    25261 tacaacctga tgatgacatg cggtatccat tccatggaca gccagatgct caatgggcat
    25321 gggatatcca tgcaacttca ggggattgat attgccgaaa ggccaggcaa gaatattcac
    25381 taacctgaac agggcctgaa aacgctgtct tttcaggcct ctgcaggcaa aaactgatga
    25441 caacaaaatt tctgcgtttg taccccaccg atgaaaaaga caaaaactca caccgggctt
    25501 ctagccagta aagataaaac ccgacgtgtc agcctctacg aaacgccaac ggcatggtgt
    25561 atcagggggc aggagtgcta cagcaaaagt acaggacgcc gctgtggcag ccacgacagt
    25621 ctgagcagac tgcgcctgga cagcattaag cctgttgaat gagtggtggc accatgaaag
    25681 ataacaacac aaccgccacc ccagaagcct ggaaaatgcg ttttctggcc aacgggacat
    25741 ccggcacatg gttttacact gaagacattg cagtcactga cgcgtggctt cgcgcgcacc
    25801 cggatgatgt ggaggttatc cctctctgcc ggatacagcc ccgccagtat tcccatgaac
    25861 aacatattga agaactccgc gcagccctgg cccggatgct gcatatgcat catatgatgc
    25921 tggaaaccac gcattacagg gaaaacatca ccgatgcccg ttgtatccgt gaaatgcatg
    25981 aagctccatt acaggcgctc aatattctgg ccagataccc gcataaaaag gacgaggtgt
    26041 gacgcatgtg gctactgatt ttctggcttt catctccctt taatgtctct tccggtgatg
    26101 cccggcagcc cacactggcg gtgcacagcc aggcatttca taccgaacgg gcctgtcgcg
    26161 atgcattgcg cgccattaaa aaggagaatg ttggcgaatt ttctctgcgg ggcgtttgtg
    26221 tttctgacgg aaatacgaag gaggaggctg agagctcatg acggatatta ctgacagaca
    26281 tacacttcgc cgtatgtcct ggtcggaact cttcacggca gcacaggaag cggaatttca
    26341 gcgtgattac gagcgtgcca gaatagtgtg gtcattcgcc ttacatgttg caacaaccac
    26401 gattaacaaa aatctggcca tagctcatat tcgacgctgc gatactttgt tgcataaatc
    26461 aaaaacagta cccggaaaca acacgggggg ccggtcagtg tgcctgagac cccaacatcc
    26521 ccggagatga ctgacattcc ggactgacgc agttgctgca tatctgtggc aatacgttgt
    26581 atccagccaa gttgcgttat ttcgtcggtt gtttcgtggg cgtagtgatg tatggggcct
    26641 tcgatgggaa agtaaaacta gcggaaaata aaagtactcg cctgcctacg gaccttttta
    26701 ctcagggtat ttttgttttc cataacgttg cgtattaaca tgaaggagaa cgagggggat
    26761 taggtaatct ctttccatga aaatacgcaa acgttcatca ccgaagtgaa aaatcagttt
    26821 ttgatattta aatgattttt caaatcatat ttcagcagga cactaccagc acctaacagc
    26881 agcacatctt tgatgacaaa actgtcaact tttcccagtt gtggtaacag acttagtgtg
    26941 actaaacctg taacaataac gataatatct cccagtatgc cagcagcagg cctgaagaag
    27001 ccgatactca gtgctagaaa agtgatagtt tccactatgc cgaggaaata gctcccccca
    27061 tgaacaccaa acataatgta caggatattc agccacgtgg gagaaatcag gggcttgagg
    27121 gccatcactt caaaatcaaa ccatttatag gtcccgaaga gcataaatat tataataaca
    27181 gacaggcgaa gaataactat gtcataatct gatttaatgt aaacattaat aaattttttc
    27241 atttcattct ccgggtggaa aattatatgc actaaaaagg attaagtacg ggtatcaagc
    27301 attttaatta aagcgttaac ggttcctgaa taggtatcat gtggtggaag tcgatttaac
    27361 atcagtgccc cctttagtgt agaacagact ataacggcga gctcatccgg aagaagaact
    27421 gttttgaact ctccactatt ctgcccggag gtgataatat ctttaatcat ctgtatctcc
    27481 tgatgtgcca gttgacttac agcctcctgt aattcaggag aaaactgatg gctgtcggat
    27541 agcatggcat aaataccgca catttcccct ctttcagcac aaccagcaaa tgcatcaaga
    27601 tatgtcctga gacgttgtac accagaagaa acattatgga gagttatatt aagtctatca
    27661 aatacttctg ttttgtattg gcagtaggct ataccaagat cagttttcct cgggaaatgg
    27721 tgatgaatac tggcttttct gataccaatt tctttttcca gatctgcgta actgaacccc
    27781 aaaaatccgc gttgctggat cagagtatcc gttatgcgaa gtatattctc ataagtagac
    27841 acggctttac ctgttatgta ttaaagggac tattatacat attttaacga tagataatgg
    27901 cagttccatc aagtttattt tttcctccca atgaagtgat tcggaaatga gaacctcctg
    27961 cctgctccgc tttgtcttta agcgcagttg ccacctcttc tggtgtatat agattaccaa
    28021 cagaaatata gtctgacggc atcaaaccaa caacgtcctg acgattaatt tcatctgcag
    28081 cagttgcgta gcctgtcaga atgagaccaa gggcgacaat ggtagtgcag ataacataat
    28141 tcatgagcag tttctcttta tgtttgcgat actacacatg ctaccttctg attggtaggc
    28201 ttgcaagatg gatttattta aaataagagg gatgatttgt atcaaattgt tttgtatttt
    28261 tgtgttttgt atcactgagg tgaagttgta agatctggcc ctctgcgagg ttggctctgt
    28321 cgaaaggaac aggtgacgag agttaacatc gttgccttgg ctgcatgggg gatatgtagc
    28381 taccccctga tatttccgcc acggaagcca atatgaacaa caataacaat acgcctcgtt
    28441 tccagtatgc ttcatgtcgg atattttgta agcgctatgt acttcccggg acgcttatct
    28501 tttagccgta gctggtagtg caataaaagc ctggcagcaa tccaggcctc tacgttctaa
    28561 gaatcaactg tcagtgcaga ggaatgcgca cgacgctctc gttgttattg gcttttgccg
    28621 tcgcaccgtc gtttttcacg gtgacaaaca gcgcgtgcgc tttgctatcc gtcttatatc
    28681 actggtgctg acaatgtgga tgaggtcgcc tttaaaaaag gacatcgtta tataacggtg
    28741 atctccgatc gcgatggtcg tgcgctggcc ttaacggacg atcgtggcac agagagcctc
    28801 gccggctatc ttcgcacgct tactgacggg cagttgctgg ctatcaaaac gctctcaatg
    28861 gacatgaatg caggctatat aagagcagcg cgtatccact taccctgtgc ggttgagaaa
    28921 atcgccttcg accgcttcca tgtagcaaag caacttggcg aagtggttga taaaacccgt
    28981 cagaatgaac atccgcacct ccctgttgaa agttggcgtc aggcaaaggg gacccgcttc
    29041 ctgtggcagt acagcgataa atggatgacc aaatccaggc aggaaaagct gatatggctg
    29101 cgtgcacaga tgaagctgac aagccagtgc tgggcgctga aagagctggc aaaggatatc
    29161 tggaacaggc catggagtga ggaaagacgt aatgactggg agagatggct ggctctggcg
    29221 gccaacagtg acgttcctat gatgaaaaac gccgcgaaaa cgataggaaa aaggctttac
    29281 gggatcctga atgccatgcg acaccgtgtc tcaaacggaa acgcggaggc gcttaacagt
    29341 aagatcaggc tactgaggat aaaagccagg ggataccgaa atcgggagcg ctttaagctg
    29401 ggcgtgatgt tccactacgg gaaactgaat atggcgttct gagccttccc accatgatca
    29461 gggaaaaccc atgtatcatc aggttattat gtgagctggt aaatcagatt ttgcatggac
    29521 tgaaatcatg ctgttattta gggataaaga accggctttt tgtcccgccc cctcgccatt
    29581 aatgaaaaaa agaatggaat gctccctctg aggagaaggg cagtagaaaa aagaacccgc
    29641 cagcaaaaat attggggatg aacagctttc gctactcaga ttgctggcgg gtatagttcc
    29701 tcatgaaata aaaatactac ataatccttt taatgcaaat gataatcatt gtcaattgtt
    29761 tgtgcgtgtt ttcttcatac atgattggta gtaaaggtga ttcaggctat cagtttttgc
    29821 tgatgatctt tttatctata gaaaacatca gccaagctgg ttggttcagt aacgctttca
    29881 tctttgagca ttttataaaa aattcttgaa ttgttaaaat agattaaagt ctggtaacag
    29941 caaaagttaa acgagaaatc aaaaggaggt cccaaatctg aaaatgaagc atctgcgtga
    30001 tgatgttgca gcgggacgtc gtcggctgta tatcagagca atctgtccat ccctgcgtga
    30061 agccccgtta ctttattcgt accccttata atggggtgtt agccagccag acccggcatg
    30121 attactgtga accgccccgg gaatcctgga gactaaattc cctgagaaag aggtaaacag
    30181 gatgactaaa aatacacgtt tttcccccga agtccgtcaa cgggcagttc gtatggttct
    30241 ggaaagtcag gacgaatatg actcacaatg ggcggcaatt tgttccatag ctccaaagat
    30301 tggctgtacg ccagagactc tgcgtgtctg ggttcgccag catgagcggg ataccggggg
    30361 cggtgatggt gggctcacca ccgctgaacg tcagcgtctg aaagagctgg aacgtgaaaa
    30421 tcgtgaactg cgccgcagta acgatatcct tcgccaggct tccgcttatt ttgcgaaggc
    30481 ggagttcgac cgcctctgga aaaaataatg ccactgctgg ataagctgcg tgagcagtac
    30541 ggggtcggac cggtatgcag tgaacagcat attgccccgt caacgtatta ccattgtcag
    30601 cagcagcggc atcatcctga taaacgcagt gcccgttgtc agcgcgatga ctggctgaag
    30661 agagagatac agcgcgtata cgatgaaaat catcaggtgt acggtgtgcg taaagtctgg
    30721 cgtcagttgt tacgggaagg aatcagggtg gccagatgta cagtggcgcg cctcatggcg
    30781 gttatgggac ttgccggtgt tctccggggt aaaaaggtcc gcactaccgt cagccggaaa
    30841 accgttgctg caggtgaccg cgtaaaccgt cagttcgtgg cagaacgtcc tgaccagctg
    30901 tgggtggctg attttactta c
//
LOCUS       DAOTRK010000050        30355 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.050, whole genome shotgun sequence.
ACCESSION   DAOTRK010000050 DAOTRK010000000
VERSION     DAOTRK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.050"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..453)
                     /gene="folK"
                     /locus_tag="QIE41_002133"
     CDS             complement(<1..453)
                     /gene="folK"
                     /locus_tag="QIE41_002133"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDS3531016.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGTVLGNLLKIN"
     gene            complement(450..1868)
                     /gene="pcnB"
                     /locus_tag="QIE41_002134"
     CDS             complement(450..1868)
                     /gene="pcnB"
                     /locus_tag="QIE41_002134"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDS3531017.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRSRRPRKRAPRREGTA"
     gene            complement(1907..2803)
                     /gene="gluQRS"
                     /locus_tag="QIE41_002135"
     CDS             complement(1907..2803)
                     /gene="gluQRS"
                     /locus_tag="QIE41_002135"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDS3531018.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2870..3325)
                     /gene="dksA"
                     /locus_tag="QIE41_002136"
     CDS             complement(2870..3325)
                     /gene="dksA"
                     /locus_tag="QIE41_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDS3531019.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3503..4207)
                     /gene="sfsA"
                     /locus_tag="QIE41_002137"
     CDS             complement(3503..4207)
                     /gene="sfsA"
                     /locus_tag="QIE41_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDS3531020.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4222..4752)
                     /gene="thpR"
                     /locus_tag="QIE41_002138"
     CDS             complement(4222..4752)
                     /gene="thpR"
                     /locus_tag="QIE41_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDS3531021.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4826..7255
                     /gene="hrpB"
                     /locus_tag="QIE41_002139"
     CDS             4826..7255
                     /gene="hrpB"
                     /locus_tag="QIE41_002139"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDS3531022.1"
                     /translation="MSSLPVAAVLPELLTALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     SAIHGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVIISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7349..9883
                     /gene="mrcB"
                     /locus_tag="QIE41_002140"
     CDS             7349..9883
                     /gene="mrcB"
                     /locus_tag="QIE41_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDS3531023.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIANMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10103..12292
                     /gene="fhuA"
                     /locus_tag="QIE41_002141"
     CDS             10103..12292
                     /gene="fhuA"
                     /locus_tag="QIE41_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDS3531024.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVGHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12343..13140
                     /gene="fhuC"
                     /locus_tag="QIE41_002142"
     CDS             12343..13140
                     /gene="fhuC"
                     /locus_tag="QIE41_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDS3531025.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13140..14030
                     /gene="fhuD"
                     /locus_tag="QIE41_002143"
     CDS             13140..14030
                     /gene="fhuD"
                     /locus_tag="QIE41_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDS3531026.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14027..16009
                     /gene="fhuB"
                     /locus_tag="QIE41_002144"
     CDS             14027..16009
                     /gene="fhuB"
                     /locus_tag="QIE41_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDS3531027.1"
                     /translation="MSKRIALFPALLLALLVIVAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16044..17324)
                     /gene="hemL"
                     /locus_tag="QIE41_002145"
     CDS             complement(16044..17324)
                     /gene="hemL"
                     /locus_tag="QIE41_002145"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDS3531028.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17549..18970
                     /gene="clcA"
                     /locus_tag="QIE41_002146"
     CDS             17549..18970
                     /gene="clcA"
                     /locus_tag="QIE41_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDS3531029.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGVIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18994..19059
                     /locus_tag="QIE41_002147"
     CDS             18994..19059
                     /locus_tag="QIE41_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531030.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19052..19396
                     /gene="erpA"
                     /locus_tag="QIE41_002148"
     CDS             19052..19396
                     /gene="erpA"
                     /locus_tag="QIE41_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDS3531031.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19443..20066)
                     /locus_tag="QIE41_002149"
     CDS             complement(19443..20066)
                     /locus_tag="QIE41_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDS3531032.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20104..20904)
                     /gene="btuF"
                     /locus_tag="QIE41_002150"
     CDS             complement(20104..20904)
                     /gene="btuF"
                     /locus_tag="QIE41_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDS3531033.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20897..21595)
                     /gene="mtnN"
                     /locus_tag="QIE41_002151"
     CDS             complement(20897..21595)
                     /gene="mtnN"
                     /locus_tag="QIE41_002151"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDS3531034.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21679..23196
                     /gene="dgt"
                     /locus_tag="QIE41_002152"
     CDS             21679..23196
                     /gene="dgt"
                     /locus_tag="QIE41_002152"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDS3531035.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23326..24750
                     /gene="degP"
                     /locus_tag="QIE41_002153"
     CDS             23326..24750
                     /gene="degP"
                     /locus_tag="QIE41_002153"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDS3531036.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24905..26062
                     /gene="cdaR"
                     /locus_tag="QIE41_002154"
     CDS             24905..26062
                     /gene="cdaR"
                     /locus_tag="QIE41_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDS3531037.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26116..26502)
                     /locus_tag="QIE41_002155"
     CDS             complement(26116..26502)
                     /locus_tag="QIE41_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDS3531038.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26815..27639)
                     /gene="dapD"
                     /locus_tag="QIE41_002156"
     CDS             complement(26815..27639)
                     /gene="dapD"
                     /locus_tag="QIE41_002156"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDS3531039.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27670..30342)
                     /gene="glnD"
                     /locus_tag="QIE41_002157"
     CDS             complement(27670..30342)
                     /gene="glnD"
                     /locus_tag="QIE41_002157"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDS3531040.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEELIEARTEFIDQLLQRLWIDAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
BASE COUNT         7007 a         7637 c         8387 g         7324 t
ORIGIN      
        1 atttattttt aataaatttc ctaatactgt tccatcagga aacaccagct ccggcgcaat
       61 ttcaaacagc ggccacagca taaatccacg attcttcata tcgtagtgcg gaacggtcag
      121 gcgttcggta tttattactt cattaccaaa tagcatgatg tcgagatcca gtgtgcgcgg
      181 cccccagcgt tccgctttac ggacgcgacc ttgttgtaac tcaatacgtt gtgtgtggtt
      241 gagtaactct tcaggtgcaa gagaggtttc cagcgccacg gctgcgttta agtaatcggg
      301 ttgatcttgc ggccccagcg gtggggtgcg gtaaaacgaa gaaacggcaa gaatgtggct
      361 ttcagggata tcgcctaatg ctttcagggc agcattgacc tgctccagcg gagaggccag
      421 attgctgcct atggcaatat acgccactgt catgcggtac cctcacgacg cggtgcgcgt
      481 ttgcgtggac gacgagaacg gcgacgcggt gacggctctt catccagctc gttgagcatc
      541 cctttttgat ctggtggcgc ggaaacctgg aactcccccc accatttcac aagacgctgc
      601 agttcagcgt tacgttcaac ttcagctcgc aaggccaaca ggtcataagc cgcacggaac
      661 ttaggatgct ccagcagttt ccaggcgcgt ttaccctgac gtcgggacat acgcaactgc
      721 aactgccaga tatcgcgggt taacgtcgtc agacgtttcg ggatcgccag tgaacggcag
      781 gcttcgtcca gcacgtcgtt catcgccagc gcgaaagcgt cgtgataggt caggccgctt
      841 tcctgggcga tcttctgtgc cgtctccagc agtgggtacc agaacatggc ggcaaacagg
      901 aacgccgggt tcacgcgcat atcgttatgg atacgcgtat cggtattctt cagcacctgt
      961 tcaatgatcc gctccatcgg gctgtcgcca ttttccgtga agtagcgggt aatggtcggg
     1021 aacagcggct ggaacaggtg atattcacac agcagcttat aggtttcgta accgtagccc
     1081 gcttgcagca gtttcaaaga ttcttcaaac agacgtgccg gtggaatatc gttcagcagg
     1141 gtagcgaggc gagggatcgg ttctgcggtt tcaggactga tacgcatacc caatttggcg
     1201 gcaaaacgta ccgcgcgcag catccgtacc ggatcttcac ggtagcgcgt ttccgggtta
     1261 ccaatcagac ggataacgcc gtctttcaga tccttcatgc cgccaacgta atcacggacg
     1321 gtaaaatccg ctacgctgta atacaggctg ttgatagtga aatcgcggcg ctgggcgtct
     1381 tcttcgatgg agccgaaaat gttgtcgcgc agcaacatac cgttttgccc gcgttgggag
     1441 gtcgtgcggt cgctgacgtt accttcgtga tgtccacgga aggtcgcaac ttcgataatc
     1501 tccgggccaa acatcacatg agccagacgg aaacggcgac ccaccaggcg gcagttacgg
     1561 aacagtttac gtacctgttc aggcgtggcg ttagtggtta cgtcaaaatc tttcggcttt
     1621 ttgccaagta acaggtcgcg cacgccgccg ccaaccagcc aggcttcgta tcccgcttta
     1681 ttgagcctgt acattacctt cagggcattt tcactgatat ctttgcggga aatagcatgc
     1741 tgctcacgcg ggatcaccgt cacctgtgga cgggcgactg cctgttcagc ctcgctttcc
     1801 tcgcggctta gcaccttgcg gcaaaaatta gcgactcggg taaaaatagt acacctcggt
     1861 agtgtcaaac atcattcagg acaaaaaaat agcggctaat catagctcag catgacgcat
     1921 ttgagaatgt tgaatttaca attgccgact caggcactgc ggtgagccgc cagtttttga
     1981 cggctgactg aaggatttgc tcgacgctga aatcctgcca gtgtgcttct gcctgttgac
     2041 ccagaaattg aagtgccgcg attaaaaccg ggcgtggatc gcctttcggc aacgcaggcg
     2101 catgattctg cttggaaagt ttagcgcctt gtggattaag cgccagcggt aaatggatgt
     2161 aatctggcac tttccagcca aaaagctggt acagcgagat ttgccgcact gtcggttcga
     2221 tcaaatctgc cccgcggaca atttcgctca cgccctggaa gtgatcatcc accaccaccg
     2281 ccaggttata ggcaaacaaa ccatcacggc gatgaataat aaaatcttcc cgtgccagtt
     2341 tttcgtcggc gtgaataatg cctcgcagca ggtcagtaaa ctgggtgacc ggatgctgct
     2401 ggcggatacg tactgcggcg ttgtctggtc catgatgtaa ttcccggcaa tgaccgtcgt
     2461 aaataccgcc aatgctttga atacgcgcac gcgtgcaggt gcagtaataa cttaatcctt
     2521 gctcatgtaa ccaggcgagt gcttcgcgat aggcgtggtg acgttgcgat tgccagagaa
     2581 catcgccgtc ccagtgcagg ccgtaatgtt ccagctggcg caggatagtt tctgcggcac
     2641 cgggaacttc acgaggcggg tcgatatctt caatgcgtac cagccagcga ccgtgcctag
     2701 cgcgagcctg taaatagctg ccaagcgcgg caatcagaga gccaaaatga agctcgccgg
     2761 aaggagaggg ggcaaagcgg ccaatatagt gtgtgtctgt catctcgttg aacaaaaaat
     2821 aaggcgggag catttcccgc ctgtggtaaa cgtgatggaa cggctgtaat tagccagcca
     2881 tttgtttttc gcgaatttca gccagcgttt tgcagtcgat gcacagatcg gctgtcgggc
     2941 gcgcttccag acggcgaata ccaatttcaa caccgcagga ttcgcagtag ccgaaatctt
     3001 cgtcttccac ttttttcagc gttttctcga tctttttgat cagcttacgc tcgcgatcgc
     3061 ggttacgcag ttcgaggctg aactcttctt cctgggctgc acggtctacc gggtccggga
     3121 agttggctgc ttcatcctgc atatgtgtaa cggtgcgatc gacttcatcc ctgagttgat
     3181 tacgccatgc ttccagaata cgacggaagt gcgccagctg ggcttcattc atatactctt
     3241 cgcccggctt ctcctgatat ggttccaccc cagcgatggc gagaatactc agggacgatg
     3301 ttttacggtt ttgcccttct tgcatgttgc ttctccttaa cacgcactat cgatccccat
     3361 gttcggggga aaaatgaggc cgctataaat agcagatgct tttccggata gcaattatct
     3421 aaacgtaaca cttgacaact gtgtgaggaa aagcgtattt gcgcacgcga ccagaatgta
     3481 aattaaccag ttacttactc tactacaatg taaccggtaa tgatttttta agagccatgc
     3541 cttcggcaga aagttccgct ttgtaagcca gaatttctac ccccctctgt tgagcttctg
     3601 acaatagttg cgcgtatttc tcatcgatgt ggcgcgcggg tgaaaaccgt gtaatggctg
     3661 aatgcagcac ggcaaaaaag ataaccgcac gctggccttc agccgctacg ctcatcaact
     3721 cccgaaggtg tttctgacct cgttcagtga ccgcatcggg aaaatatccc tgttcgtttt
     3781 ccgctaacgt aaccgatttc acttcaatat agcagtctgg acgcgaatcc gcctgcaaca
     3841 taaagtcaat acggctgcgt tctgcgccgt atttaacttc gcttttcagc gagctatagc
     3901 ctgacagttc tgaaattgat tcattaagga tagcctcttt cgtcaacctg ttagcccaaa
     3961 gcgtattgac gcaaataatt gcgccgctct ggctttgagt caattcccag gtgtgtgggt
     4021 atttccgttt ggtgttgtct gaagtcgaat accagacggt atcgccaggc gttgcacaac
     4081 cggtcatcgc acccgtattc gggcagtgta gcgttaattc gcgaccatcg ggtgtgatca
     4141 catcggctaa aaaacgtttg taacgctgaa ttagcgtcgc gcgctgtaga gggggagaaa
     4201 attccattac gacaatcctt gttattgcgt tagcgcccag cgttttagcg gcgtgtagcg
     4261 tgtgcgtcca cgcgcaaacg aggaggcata aagggtgaac tccgtcaccg tatacgacca
     4321 gttaaaacct ggcggcggga ttgtcaccgc ctcgctggcg tcgcgcaata aggtaatatg
     4381 tggatgaaac ggacgattgc tttgaaaaca accgctgcgg gcagcctgtg aacggagcat
     4441 attcgccagc tggattaagc cgcgtggtgg ctgacgcatc cctaaccaca ccacacgcga
     4501 acgcagccat tgaccggcgt catcaagcgt gagtgtgaaa cccggttgac gaatccgccc
     4561 ggctaaaaga gaaagcgcct tctctttctc ggcgctcact tcgcctaaaa atgccagagt
     4621 cagatgcaaa ttatcggcgg cgaccggacg tcccgcgtca ggtgggaagt gtgtggcgcg
     4681 ccagtggata atctgttcgc ggatttctgc aggtaagtcg atagcaaaga acagccgttg
     4741 cggttcagac atgtgaggca ctcggttatg aattaccgcg atgctacaat gtgccgcgaa
     4801 gaatgttaac cctctggagc gttttgtgtc gtcgttgccc gttgctgccg tcttacctga
     4861 attacttacc gctctcgatt gtgcatcgca ggtgttatta agtgcgccga ccggggccgg
     4921 gaaatcaacc tggctgccgc tgcaactgct ggcgcattca gctattcacg ggaaaatcat
     4981 tctgctggag ccgcgtcgtc tggcggcgcg taacgttgcg caacggctgg cggagctgct
     5041 taacgaaaag ccaggcgata ccgttggcta ccggatgcgt gcgcaaaact gcgtcgggcc
     5101 gaatacccgc ctggaagtgg ttaccgaagg cgtgctgacg cgcatgatcc agcgtgaccc
     5161 ggaactgagc ggtgttggat tggtgatcct cgatgagttt catgagcgca gcttgcaggc
     5221 ggatttggcg ttggcgctgt tactcgatgt gcaacaaggt ctgcgtgatg accttaaact
     5281 gctgattatg tcggctacgc tggacaacga ccgcttgcag caaatgctgc cagaagcgcc
     5341 cgtcatcatc tcagaagggc gctcgtttcc ggttgaacgc cgttatttac cgctgcccgc
     5401 gcatcagcgt tttgacgaag ccgttgcggt agccaccgcc gaaatgctgc gtcaggaaag
     5461 tggatcatta ctgttatttt tacctggcgt cggagaaatt cagcgcgtgc aggaacaact
     5521 ggcttcgcgc atcggcagtg atgtgttgct ctgcccgctg tatggcgcgt tgtcgctgaa
     5581 cgatcagcga aaagcgatcc tcccggcacc gcaagggatg cgcaaagtgg tgctggcgac
     5641 caatattgct gaaaccagtt taaccattga aggtattcgt ctggtggtgg attgtgccca
     5701 ggagcgtgtg gcgcgttttg atccgcgcac ggggcttacg cggttgatta ctcaacgcgt
     5761 tagtcaggca tccatgacgc agcgtgccgg gcgcgccggg cgtctggagc cgggtatctg
     5821 cctgcattta atcgccaaag aacaagcaga acgcgctgcg gcgcaaagtg aaccggaaat
     5881 attacaaagc gatctttccg gtttgctgat ggaattactg caatggggat gcagcgatcc
     5941 ggcgcagatg agctggctgg atcaaccgcc aacggtgaat ctactggccg cgaaacgcct
     6001 gttacggatg ctgggggcgc tggacggtga acggcttagt gcgcaagggc aaaaaatggc
     6061 agcgctgggt aacgatccgc gtttagcggc aatgctggtt agcgcgaaga acgacgacga
     6121 agctgctacc gcggcaaaaa ttgccgccat tctcgaagag ccgccacgga tgggcaatag
     6181 tgacctgggc gtggcgtttt cgcgcaatca accagcctgg cagcaacgta gtcagcaact
     6241 gttaaaacgt ttaaacgtac gcggcggtga ggcagacagt tctcttatcg cgccgctact
     6301 tgccggggcg tatgccgatc gcattgctcg tcgccgtggg caagatggac gctatcaact
     6361 ggcaaacggc atgggggcga tgctcgatgc cgacgatgcg ctaagccgcc acgaatggtt
     6421 gatcgcaccg ttattattgc agggcagcgc ctcgccggat gcgcggattt tactggcgct
     6481 gccggtcgat attgatgagt tagtacaacg ctgcccgcag ctggtacagc agtcggacac
     6541 cgtggaatgg gatgacgcgc aaggcacgct gaaagcctgg cgtcggctgc aaatcggtca
     6601 gttgacggtg aaagtgcagc cactggcgaa accctcggaa gacgagttgc atcaggcgat
     6661 gcttaacggc attcgtgata aaggtttaag cgtgctcaac tggacggcgg aagcggaaca
     6721 gctacgcttg cgtttgttat gcgccgcaaa gtggttgccg gaatatgact ggccagcggt
     6781 tgatgatgag agtttgttgg cgacgctgga aacgtggcta ctgccgcata tgagcggcgt
     6841 gcattcacta cgcggcctga aatcactcga catttatcag gcactacgcg gattacttga
     6901 ttggggaatg cagcaacgtc tggatagtga attgcctgcg cattacactg tgccgacggg
     6961 aagccggatc gccattcgtt atcatgaaga taacccgccc gcgctggcgg tgagaatgca
     7021 ggagatgttt ggcgaggcca ccaatccgac gatcgctcag gggcgcgtgc cgctggtgct
     7081 ggagttgctt tcccctgccc aaaggccgct gcaaatcacg cgtgatttgg gggccttctg
     7141 gaaaggggcg tatcgtgagg tgcaaaagga gatgaaaggg cgttatccca aacacgtctg
     7201 gccggacgac ccggcaaaca cggcaccgac gcgacggacg aaaaagtatt cgtgaagacc
     7261 aaagcaaatt tgagagatat cttcttctgt cttgcaacag aagaacagaa aatcgggctt
     7321 ttgcgcctga atattgcgga gaaaaagcat ggccgggaat gaccgcgagc caattggacg
     7381 caaagggaaa ccgacgcgtc cggtcaaaca aaaggtaagc cgtcgtcgtt acgaagatga
     7441 cgatgattac gacgattatg atgactatga ggatgaagaa ccgatgccgc gcaaaggtaa
     7501 gggcaaaggc aaagggcgta agcctcgtgg caaacgcggc tggctatggc tgctgctaaa
     7561 actggctatc gtttttgccg tgctgatcgc catttacggc gtttatctcg atcaaaaaat
     7621 tcgcagccgt attgatggca aggtctggca actgcctgcg gcagtttatg gccgaatggt
     7681 caaccttgag ccagatatga ccatcagcaa gaacgagatg gtgaagctgc tggaggcaac
     7741 ccagtatcgt caggtgtcga aaatgacccg tcctggcgaa tttaccgtgc aggccaacag
     7801 cattgagatg atccgccgtc cgtttgattt cccggacagt aaagaagggc aggtgcgtgc
     7861 gcgtctgacc tttgatggcg atcatctggc gaccatcgtc aatatggaga acaaccgtca
     7921 gtttggtttc ttccgtcttg atccgcgcct gatcaccatg atctcctcgc caaacggtga
     7981 gcaacgtctg tttgtgccgc gcagtggttt ccctgatttg ctggtggata ctttgctggc
     8041 gacagaagac cgccatttct acgagcatga tgggatcagt ctctactcca tcggacgtgc
     8101 ggtgctggca aacctgaccg ccggacgcac ggtgcagggg gcgagtacgc tgacgcaaca
     8161 gctggtgaaa aacctgttcc tctccagcga gcgctcttac tggcgtaaag cgaacgaagc
     8221 ttacatggcg ctgatcatgg acgcgcgtta cagcaaagac cgtattcttg agctgtatat
     8281 gaacgaggtg tatctcggtc agagcggcga caacgaaatc cgcggcttcc cgctggcgag
     8341 cttgtattac tttggtcgcc cagtagaaga attgagcctc gaccagcagg cgctgttggt
     8401 gggtatggtg aaaggggcgt ccatctacaa tccgtggcgt aacccgaaac tggcgctgga
     8461 gcgacgtaat ctggtactgc gtctgctgca acagcaacag attattgatc aagaactcta
     8521 tgacatgttg agtgcacgtc cgctgggcgt tcagccgcgc ggtggggtaa tctctccaca
     8581 gccagccttt atgcagctgg tgcgtcagga gctgcaggca aaactgggcg ataaggtaaa
     8641 agatctctcc ggcgtgaaga tcttcactac ctttgactcg gtggcccagg acgcggcaga
     8701 aaaagccgcc gtggaaggca ttccggcact gaagaaacag cgtaagttga gtgatcttga
     8761 aaccgcgatt gtggttgttg accgctttag cggtgaagtt cgtgcgatgg tcggtggttc
     8821 tgagccgcag tttgcgggct acaaccgtgc gatgcaggcg cgtcgttcga ttggttccct
     8881 tgcaaaacca gcgacttatc tgactgcctt aagccagccg aaaatctatc gtttgaatac
     8941 gtggattgcc gatgcgccaa tcgcgctgcg tcagccgaat ggccaggtct ggtcaccgca
     9001 gaatgatgac cgtcgttata gcgaaagtgg cagagtgatg ctggtggatg cgttgacccg
     9061 ttcgatgaac gtgccgacgg tgaatctggg gatggcgctg gggctgcctg cggttacgga
     9121 gacctggatt aaactgggcg taccgaaaga tcagctgcat ccggttccgg caatgctgct
     9181 gggggcgttg aacttaacgc caatcgaagt ggcgcaggca ttccagacca tcgccagcgg
     9241 tggtaaccgt gcaccgcttt ctgcgctgcg ttcagtcatc gcagaagatg gcaaggtgct
     9301 gtatcagagc ttcccgcagg cagaacgcgc tgttccggcg caggcggcat atctgacact
     9361 atggaccatg cagcaggtgg tacaacgtgg tacgggtcgt cagcttgggg cgaaataccc
     9421 gaacctgcat ctggcaggga aaacagggac taccaacaat aacgtagata cctggtttgc
     9481 gggcattgac ggcagcacgg tgaccatcac ctgggtaggc cgtgataaca accaaccgac
     9541 caaactgtat ggagccagcg gggcaatgtc gatttatcag cgttatctgg ctaaccagac
     9601 gccaacgccg ctaaatcttg ttccgccaga agatattgca aatatgggcg tggactacga
     9661 cggcaacttt gtttgcagcg gtggtatgcg tgtcttgccg gtctggacca gcgatccgca
     9721 atcgctgtgc cagcagagtg agatgcagca gcaaccgtca ggcaatccgt ttgatcagtc
     9781 ttctcagccg cagcaacagc cgcaacagca acctgctcag caagagcaga aagacagcga
     9841 cggtgtagcc ggttggatca aggatatgtt tggtagtaat taacgtctaa gcgtgacata
     9901 ccggatggcg agttgccatc cggtaaaata acatcccacc taaagtatta accctttctt
     9961 ttcatctggt tgtttattaa cccttcagga acgctcagat tgcgtaccgc ttgcgaaccc
    10021 gccagcgttt cgaatattat cttatcttta taataatcat tctcgtttac gttatcattc
    10081 actttacatc agagatatac caatggcgcg ttccaaaact gctcagccaa aacactcact
    10141 gcgtaaaatc gcagttgtag tagccacagc ggttagcggc atgtctgttt atgcacaggc
    10201 agcggttgaa ccgaaagaag acactatcac cgttaccgct gcacctgcgc cgcaagaaag
    10261 cgcatggggg ccagctgcaa ctattgcggc gaagcactct gctactgcga ctaaaacgga
    10321 tacaccaatt gaaaaaacgc cgcagtctat ttcggttgtg acaaatgaag agatgcagat
    10381 gcatcaattt cagtctgtaa aagaagcatt aggttataca cctggcgtta cggttagcag
    10441 tcgtggtgct tctaatacat atgattttgt tattattcgt ggtttctcat ctgttggtct
    10501 gaatcaaaat aattaccttg atgggctaaa acttcagggc aacttttata acgatgctgt
    10561 gattgatcct tacatgctcg agagggttga actgatgcgc gggccgacgt ctgttctcta
    10621 tgggaaaagt aatcctggtg ggattatttc tatggtgagt aagcgtccga caactgaacc
    10681 cctgaaagaa attcagttta aaatggggac ggataatctg tttcagaccg gattcgattt
    10741 cagtgatgcg ctggatgata acggtgagtt ctcctaccgt ttgactggcc tcgcacgctc
    10801 aacaaacgaa cagcagaaaa actctgaatc tcagcgttat accattgctc catcattctc
    10861 atggcgtcca gacgacaaaa ccaattttac tttcctgtcc tattttcaga acgaaccaga
    10921 aacgggttat tacggttggt tgccgaaaga ggggaccgtt gagccattgc ctaatggtaa
    10981 gcgtctaccg actgatttca atgaaggtgc atcgaataat acatactccc gtaaccagaa
    11041 aatggtggga tatagttttg aacatggttt taatgacacc ttcaccgtgc gtcagaatct
    11101 gcgtttcagt gaaatgaaaa cctcacagaa aagtgtttat ggcacaggga ttgccagcga
    11161 tggtcatacc ttaaaccgcg ggacagtggt ggataatgag cgtctacaaa actttagcgt
    11221 tgatacccaa cttgaaagta agtttgctac aggtgacgtc gggcatactt tgctgacagg
    11281 ggtagacttc atgcgtatgc gcaatgatat caatgccagc tttggatctg caccatccat
    11341 cgatctttat aacaaatacc atcctgaata ctttgcattt ggtagcgcag agccatacca
    11401 gatgaatgaa agcaaacaaa caggtattta tgttcaggat caggcggaat ggaataaatg
    11461 ggtattcact ctggggggac gctacgattg gtctaagcaa gcgaccactg ttcgtgaaaa
    11521 ctcttatacg ccgactgaag gttatattga gcgcaatgat catcagttca cctggcgcgg
    11581 tggtgtaaat tacttattcg ataatggtat ttcaccttac tttagctata gccagtcctt
    11641 tgaaccgagt gctttcgatc tgtggagcaa cccgcgcgtt tcctataagc catcgaaagg
    11701 tgaacagtat gaagctggcg taaaatatgt tccgaatgat atgccggtcg ttgttacggg
    11761 cgcagtctat caattgacga aaacaaataa cctgacagca gacccaacaa acccattagc
    11821 gcaagtcccg gcaggtgaga ttcgcgctcg tggtgtggaa cttgaagcaa aagcggcgtt
    11881 aaatgccaat attaacttga cggcttctta tacctacacc gatgcggaat acaccaaaga
    11941 caccaatctc aaaggtaaaa ctccagaaca agtaccggag catatggcat ctctctgggg
    12001 ggattatacc ttcaatgaag ggccgctttc tggtttaaca ttgggaacag gtggtcgttt
    12061 tattggttcc agctatggtg atccggcaaa cacttttaaa gtgggtagcg cagctgtaat
    12121 ggatgctgtt gtaaaatatg atctggcacg ctttggtatg gcgggatcca gccttgctgt
    12181 gaacgtcaac aatttgctcg atcgtgagta tgttgccagt tgcttccaga cctatggctg
    12241 cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc ttccgtttct aatttctctt
    12301 ttggggcacg gatttccgtg cccgtttcac aagttggctg ttatgcagga atacacgaat
    12361 cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg gcgcacgctt
    12421 ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat tggtcacaac
    12481 ggttctggta aatccactct gctcaaaatg cttggtcgtc accagccacc gtcggaaggg
    12541 gagattcttc ttgatgccca accgctggaa agctggagca gcaaagcgtt tgcccgcaaa
    12601 gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgacagtgcg tgaactggtg
    12661 gcgattggtc gttacccgtg gcatggcgca ctagggcgct ttggtgcggc agatcgcgaa
    12721 aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg gctggtcgat
    12781 agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc acaggatagc
    12841 cgttgtctgt tgctcgacga accgacctcg gcgctggata tcgcccacca ggttgatgtg
    12901 ctggcgctgg tgcatcgttt aagtcaggag cgtggcctga cggtcattgc cgtgctgcac
    12961 gatatcaata tggctgcgcg ttactgtgat tatctggtcg ccctgcgcgg cggtgaaatg
    13021 attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat gatttatggc
    13081 atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt tgtttattga
    13141 tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg ctttctccgt
    13201 tgttatggca gatgaatact gcccacgcgg cggctattga tcccaatcgt attgtggcgc
    13261 tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac ggcgtggcgg
    13321 ataccatcaa ctatcgcctg tgggtcagcg aaccgccatt gccggactca gtgattgacg
    13381 ttggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg tcgtttatgg
    13441 tctggtcggc aggatatggc ccttcaccag aaatgctggc acgcattgcg ccggggcgcg
    13501 gatttaactt cagcgacggc aaacagccgc tggcgatggc gcgtaagtca ctgacggaaa
    13561 tggcagattt acttaacctg caaagcgcag cagaaacgca tttagcgcac tatgaagact
    13621 ttatccgcag catgaaaccc cgctttgtga agcgtggtgc gcgcccgtta ttgctgacga
    13681 cacttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc caggaaattc
    13741 ttgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg ggcagtaccg
    13801 ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt tttgatcacg
    13861 acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc atgccgtttg
    13921 tccgtgcagg acgttttcag cgcgttccgg cggtctggtt ctatggcgcg acgctttcgg
    13981 caatgcactt tgtgcgtatt ctggataacg ccatcggagg taaagcgtga gtaaacgaat
    14041 tgcgcttttc ccggcgttat tgctggcgct gctggttatt gtcgcagcgg cgctcacctg
    14101 gatgaacttt tcgcaagcgc tgccgcgtag ccagtgggcg caggctgcct ggtcgccgga
    14161 tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc tggcgatttc
    14221 gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag tgctgcgtaa
    14281 cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg ggattaccgt
    14341 cactacgctc tgggcgatcc caggtgctat ggcgagccag tttgctgcgc tggcaggggc
    14401 ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt cgccggtaac
    14461 gctgattctc gcggggctgg tagtgagcct ttattgtggg gcaatcaatc agttactggt
    14521 tatcttccat catgaccagt tgcaaagcat gttcctgtgg agcaccggaa cgctgacgca
    14581 aaccgactgg ggcggcgttg agcgtttatg gccgcagttg ctgggcggcg tgatgctgac
    14641 gttactgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg cgcgcaacct
    14701 cgggctggcc ttgtcgctcg cgcgtctggc ggcgctgtcg ctggcgatag tcatcagtgc
    14761 gctgctggtg aacgcagtgg ggattatcgg ctttatcggg ttgttcgcgc cactgctggc
    14821 aaaaatgctg ggggcgcggc gtttgttgcc acgattgatg ctggcgtcgc tgattggggc
    14881 gttaattctg tggctttctg atcaaatcat cctctggctg actcgcgtgt ggatggaagt
    14941 ttctaccggt tcggtcactg cgttgatagg tgcgccgctg ctactttggc tgctgccgcg
    15001 tttacgcagc attagcgcgc cggatatgaa ggtgaacgat cgtgtcgctg ctgaacgcca
    15061 acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgctg ttgatggcag tggtggtggc
    15121 gctgtcgttt ggccgtgatg cgcacggctg gacgtgggcg agcggggcgt tgctcgagga
    15181 tttgatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggag tcatgctggc
    15241 ggtggcgggc tgtattattc agcggctgac cggaaacccg atggcaagcc cggaagtgct
    15301 ggggattagc tccggcgcgg cgtttggcgt ggtgttgatg ctgtttctgg tgccgggtaa
    15361 tgcctttggc tggctgttac ctgcaggcag tctcggcgcg gcggtgacgc tgttgatcat
    15421 tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg cggggatggc
    15481 gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg acccgcgaat
    15541 ggcgcaagtt ctgacctgga tttccggttc gacctacaac gcgaccgatg cgcaggtctg
    15601 gcgtaccgga attgtgatgg tgattttgct ggcgattacc ccgctgtgcc gccgctggct
    15661 gaccatttta ccgctgggag gtgataccgc acgagccgtg gggatggcgc tgacgccgac
    15721 gcgaattgcg ctgctgctgt tagcggcttg cctgacagcg actgcgacaa tgaccattgg
    15781 accgttgagt tttgttggtt taatggcacc acatattgcg cggatgatgg gctttcgacg
    15841 gacgatgcca cacatcgtaa tttcggcgct ggtgggagga ttactgctgg tgttcgctga
    15901 ctggtgtggg cggatggtgc tgtttccatt ccagatccca gcggggctgc tgtcgacctt
    15961 tatcggcgcg ccatatttta tctatttgtt gagaaagcag agccgttaat tgaatgcctc
    16021 gcccggtggg cgaggcaaaa tcatcacaac ttcgcaaaca cccgacgtgc agcatcgatg
    16081 gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc aaacgctgac
    16141 ggtgccagat aaacaccttc gtccagcatc atatggaaga aacgcttaaa gcgttccacg
    16201 tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa gaaaataccg
    16261 aacatgccgc caacgtggtt taccaccagc ggaattccgg cttcttctgc cgcttccagc
    16321 agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaacgcc cggctgcgcg
    16381 acttcattca gacaggcgaa accagcagcc atcgcaatcg ggttaccgga aagcgtaccc
    16441 gcctgataga ccggaccggt cggggccagc gcatccatta catcacgacg accaccgaat
    16501 gcgcctaccg gcattccacc gccgatgatt ttgccgaggc aggtgagatc cggttccaca
    16561 ccgtaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac ttcatcgatg
    16621 atcagcaacg cgccaaattc gtcgcacagc gcgcgcagac ctggcaggaa ctctggcagc
    16681 ggtggaacgc agttcatatt gcctgccacc ggctcgacga taatacaggc aatctcttgc
    16741 gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca ggttaaggta
    16801 tgtttggcga aatctgccgg aacgcccggc gaatttggct ggcctaacgt gagtgcgcca
    16861 gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc aaatttaata
    16921 attttgtcgc gaccggtaaa accacgggcc aggcggatgg cactcatggt cgcttcagtg
    16981 ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac cagttgcgcc
    17041 attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc ggcttcaatc
    17101 acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca ggaaccgaca
    17161 taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc tttttcgata
    17221 aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac accgccaggg
    17281 atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg ttcctgattc
    17341 gtagaaaaag tgaatggctg ctattctatg ttattcataa caagttaaat actcgtcaaa
    17401 catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt tttgtgcatt
    17461 tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg ccgtcatctt
    17521 atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt tagaaacacc
    17581 gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc gcgataaaac
    17641 cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc tggctgcggt
    17701 tgcttttgac aaaggtgtcg cctggttgca gaaccagcgt atgggggcgc tggtacatac
    17761 tgctgataat tatccgcttc tgttaaccgt cgcttttctc tgttcggcgg tgctggcgat
    17821 gttcggctac tttttggtgc gcaaatacgc gccggaagca ggtggttcgg ggatcccgga
    17881 aattgaaggg gcgctggaag atcaacgtcc cgttcgctgg tggcgtgtgt tgccggtgaa
    17941 attctttggc gggctgggga cactcggcgg aggcatggtg ttggggcgcg aagggccaac
    18001 cgtacagatc ggcggcaata tcggtcgcat ggtgcttgat attttccgcc tgaaaggtga
    18061 tgaagctcgc catacgctgt tggcaaccgg tgcggcggcg ggtctggcgg cggcctttaa
    18121 cgccccgcta gcgggtattt tgtttattat cgaagagatg cgtccgcagt ttcgctatac
    18181 gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta tgtaccggat
    18241 ttttaatcat gaagttgcat tgattgacgt cggtaaactt tctgacgcgc cgcttaatac
    18301 gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta tttttaataa
    18361 atgggtgctg gggatgcagg atttgctgca ccgtgttcac ggcggcaata ttaccaaatg
    18421 ggtactgatg ggcggtgtaa ttggcggtct gtgtggattg ctggggtttg tggcaccagc
    18481 aacgtcgggc ggcggcttta acctgattcc tatcgctacc gcagggaatt tcagcatggg
    18541 aatgctggtg tttatcttcg tcgcgcgggt cattaccacc ttactctgtt tctcttccgg
    18601 cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg gaaccgcttt
    18661 cggaatggtt gccgttgagc tgtttccgca atatcacctt gaagcaggca cttttgctat
    18721 tgccggaatg ggggcgttac tggcggcatc tattcgcgcg ccgttaacgg gaatcattct
    18781 ggttctggaa atgaccgata actaccagct cattttgcca atgattatta ccggtcttgg
    18841 cgcaacacta ttagcacaat ttaccggcgg gaaaccgcta tactcggcga ttcttgcgcg
    18901 cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat cagccagcga
    18961 gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg gttagaattt
    19021 gcccaattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac tgccgctgga
    19081 gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag ataacccgaa
    19141 tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt atggtttcac
    19201 ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg ttggcctggt
    19261 ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactata ccgaaggtct
    19321 ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg gttgcggttc
    19381 ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca catcttctct
    19441 cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca acgaatagcc
    19501 gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc cagtggtacg
    19561 gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc agttgcgtaa
    19621 atttctgtac gtaaaatcat ggggatttcg cgggccagta catcacgaat gatcccgccg
    19681 ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc cgcattaaag
    19741 gctttattca cgccaatgcc gacaaacacc gccagaccaa cggcgtccag caccggcaac
    19801 atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat gctggtgacc
    19861 attgcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg atccagcgcc
    19921 atgtcgcgaa ttgtcccgcc gcctactgcg gtaaccacgc ccagtaccag aacaccaaaa
    19981 gggtccatac gcaattttcc ggctaacaaa acgccggaga tggcaaatac cgctgtgccg
    20041 actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg aaaaggtcag
    20101 gcgctaatct acctgtgaaa gcgcattaca gagctgttgt gcagcgagga taatacgtgg
    20161 gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga gctgttcacc
    20221 ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacta tcgcctgtgg
    20281 cgcgcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc tatctttaaa
    20341 gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt ttccactggt
    20401 aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat cagcatattg
    20461 cgctttcaat tgcgcgtact gatccagcag ggattgcgcg gcttgttcgg ccttgtctgg
    20521 ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa tgcttgtcgc
    20581 atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc gctcggcatt
    20641 acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt ccagattcat
    20701 cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt cggaatagct
    20761 gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg cgggagaaag
    20821 cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgca agaaaagaca gggcgaccag
    20881 cgccctgaac agtgacttag ccatgtgcca gtttctgcac cagtgactca accatcaggc
    20941 tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac tgttgatcgg
    21001 ccacgtcgga gatggcgcgt actacgacaa acgggacgtt gaaattgtgg cagacatggg
    21061 cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg cggatttttg
    21121 ccagaccaac agaaccgttg atgaaagcgt cgccgctgac aatcaggcca cgtacagcgt
    21181 taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg tcagctttaa
    21241 agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg acatccgcgt
    21301 cgtgataacg tgcttcgtcc gagacaacga tatcgcccac tttcagcgtt ggtgccaggc
    21361 caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac agcagagtgg
    21421 cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc tcggttccat
    21481 tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg ttttcgattt
    21541 tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt ttcatagatt
    21601 tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg gaagcgtatt
    21661 tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa ctggcatcgt
    21721 cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat cttcgagagc
    21781 gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa gacccaggtt
    21841 tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat ggaagtccag
    21901 caggtggggc gctacatcgc caaagaaatt ttaagccgtc tgaaagagct taaattgctg
    21961 gaagcatacg gcctggatga actgaccggc ccttttgaaa gcattgttga gatgtcatgc
    22021 ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcggc gataaatgac
    22081 tggtttcgcc agcgtttgca cccggaagat gccgaaagcc agcctctgac tgacgatcgc
    22141 tgcagcgtgg cagcactacg tttacgggac ggggaagaac cgcttaacga gctgcggcgc
    22201 aaaatacgtc aggacttatg tcattttgag gggaatgcac aaggcattcg cctggtgcat
    22261 acattgatgc ggatgaatct cacctgggca caggttggcg gtattttaaa atatactcgt
    22321 ccggcgtggt ggcgtggtga aacgcctgag acacatcact atttaatgaa aaagccgggt
    22381 tattatcttt ctgaggaagc ctatattgcc cggttgcgta aagaacttaa tttggcgctt
    22441 tacagtcgtt ttccattaac gtggattatg gaagctgctg acgacatctc ctattgtgtg
    22501 gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct ttatcatcat
    22561 ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt ggttgaaaat
    22621 gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca gttttttatg
    22681 tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg atttattgat
    22741 aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga tgccagcgaa
    22801 tgcagcgatc ttctaaagtt atataaaaat gtcgctgtaa aacatgtgtt tagccatcca
    22861 gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt agagatttat
    22921 cgccctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa agaacgggtg
    22981 aaacgtttcc ctattgaatc gcgcttattc aacaaactct cgacgcgcca tcggctggcc
    23041 tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct atgggaatat
    23101 tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct ctatgcctgg
    23161 gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa agacgaacaa
    23221 taaattttta ccttttgcag aaactttagt tcggaacttc gcgctataaa acgaatctga
    23281 agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa aaaaaccaca
    23341 ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct ctctgcaacg
    23401 gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc accgatgctc
    23461 gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac cgttaatacg
    23521 ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt ctgccaggaa
    23581 ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa tggcggcggc
    23641 cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga taaaggctat
    23701 gttgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca gctgagcgat
    23761 ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat cgcgctgatc
    23821 cagatccaga acccgaaaaa tctgaccgca attaagatgg cggattctga tgcactgcgc
    23881 gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac ggtaacttcc
    23941 gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga aaacttcatc
    24001 cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa cctgaacggc
    24061 gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat cggtatcggt
    24121 tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga atacggccag
    24181 gtgaaacgcg gtgagctggg tattatgggg actgagctga actctgaact ggcgaaagcg
    24241 atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa ttcctccgct
    24301 gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa gccgatcagc
    24361 agctttgccg ccctgcgtgc tcaggtgggt actatgccgg tgggcagcaa actgaccctg
    24421 ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca gagcagccag
    24481 aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat gagcaacaaa
    24541 ggcaaagatc agggcgtggt agtgaacaac gtgaaaacgg gcactccggc tgcgcagatc
    24601 ggcctgaaaa aaggtgatgt gattattggc gcgaaccagc aggcagtgaa aaacatcgct
    24661 gaactgcgta aagttctcga cagcaaacca tctgtgctgg cactgaacat tcagcgcggc
    24721 gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg aaaacgggaa
    24781 ggggttctcc ttacaatctg tgaacttcac cacaactcca tacatcttca tcatcctttg
    24841 ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc ataacggagg
    24901 acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg cacgtaccat
    24961 gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta tcggcagcgg
    25021 cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt cacagggacg
    25081 agtcgtcgat atcgatgacg cggtggcacg tcatctgcac ggtgtgcggc aagggattaa
    25141 tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgactg gtgaaccaga
    25201 gaacctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc tggaacagtc
    25261 gcggttgatg cacctgttgg cgcaggatag ccgtttgcgg gaagaactgg tgatgaacct
    25321 gattcaggct gaggagaata cccccgcact tactgaatgg gcgcaacggc tggggatcga
    25381 tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc ttggcgtgga
    25441 cagcgcaatg gcggagttac agcaactgca aaacgcgctg actacgcctg agcgtaataa
    25501 tctggtggca attgtttcgc tgaccgaaat ggtggtgttg aaaccggcgt tgaactcttt
    25561 tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta cccgcatgaa
    25621 agagtacggc cagctgcgtt ttcgcgtttc gctgggtaac tattttaccg gaccgggcag
    25681 tattgcccgc tcttatcgta cggcgaaaac gacgatggtg gtgggtaaac agcgaatgcc
    25741 agaaagtcgc tgctattttt atcaggatct gatgttgcct gtgttactcg acagtttgcg
    25801 tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag cgatggacaa
    25861 taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc aaccgctggc
    25921 tacgtcaaag gcgctgttta ttcatcgtaa tactctggag tatcggctta atcgtatatc
    25981 ggaactgacc gggctggatt tgggcaattt tgatgacagg ttgctgctgt atgtggcgtt
    26041 gcaactggat gaagagcggt aggtcgatga caaaattaca gccgggtgag actttacccg
    26101 gcccaaagaa tggcattatt tacgcgtcag tttttccaga tcggcttcaa tctcgctgat
    26161 cttattggtt actactgact ccagatgacg taagtcgtcg aggatcttac gcttaagatc
    26221 aacttcgctg cggtcacgct ggcagatttg atctaactca tcaatgatat agcgaagatt
    26281 agggctaatc tcctgcactt ctttataacc ctgacccaca ccatcggcga cgaccgtttt
    26341 acgctgacgc ggatatttaa acttcacgct cttggcgaaa aactcgcctt tgtctttctg
    26401 gaaatagatt ttcagaatat cgttgttggc ttcctgccgg aggctgtaac gatcaatttc
    26461 ttcaggattg gtaataccca gacttttcag attgtcgtac atagcgttac ctcaaaatga
    26521 gtcagtaaat tgtgcttatt ttagcatttg gcctggcccg cccggctcga ctgtgatcgc
    26581 agaaagcctt tttcttccag acttctatca tggcgtaaaa atcaaaaaat tacctgcttt
    26641 attgtggcga tattccatgg gatggattag cacatttatc tttgttttgc gaattacatg
    26701 acagtccgaa acaagatgaa aaagtattta aaaaagggga agaagaatga gtaggacgaa
    26761 ttattctgaa ccagaaagca atcgggcagc gtcgtgctgc ccgtgtgcat acttttagtc
    26821 gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt caactttctt
    26881 aacgataacc gcacagtaaa gactgtattt gccatctttc gacggcagat tgcctgaaac
    26941 aaccacagat cctgccggaa cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat
    27001 acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt cgacaattac
    27061 cccttcaacc acttcagagc gcgcgccgat gaagcagtta tcttcaataa tggtcgggtt
    27121 agcctgcagc ggctccagca cgccgccgat acccacgcca ccggaaaggt ggacgttttt
    27181 accaatctgc gcacaggaac cgacggtcgc ccaggtatca accatcgtgc cttcatcaac
    27241 atatgcgccg atgttgacgt aagacggcat cagcacggtg ttacgggcaa tgaacgcgcc
    27301 ctgacgcact gccgctggtg gcacgacgcg gaagccttct ttctggaaac gtgcttcatc
    27361 gtagttagcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc cttcgatcac
    27421 ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact gatgcgtcac
    27481 ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca gggcgatcac
    27541 ctgatttacc gcttcgcggg taactgtgtc tgcattggct ggcgtgatct cggcacggcg
    27601 ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct tttcatatca
    27661 gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct gatgcacttc
    27721 ctgctgcaac tcgttattaa gtgcacgccg gtcagcggtg gcaattatga ataaatcttc
    27781 tactcgttcg ccaatggttg taattcgggc gccatgaagc gaaattccca gatcggcaaa
    27841 aattttcccg actctcgcca gcagtcctgg ttggtcgagc gcgataagct caaggaacga
    27901 tttgcggtcg gtatgggtcg gcaaaaatgt cacttcagtt tcaacagtaa agtgacgtag
    27961 tttggccggt tggcgacgtg gctgcggtgg ctgccagcta ctttgtgtca gtacctgctc
    28021 cagaccaaat cgaataactt catggcgatc tgcggacagc gggctgccat cgggttccag
    28081 cacgatgaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg cgtcgtggac
    28141 acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg ggcggtccgg
    28201 gctccagata aaaatctcgg tgcctccacg cgtagcctgc ggactaagca ataccagcgg
    28261 tttgcttaaa tcatgctgta ataaatggcg cgcatgccag gccagttgat ttgggctatg
    28321 gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat
    28381 gttatccatg cgcagtagtg ccagcgcctg gagttggtga tggcgaaccc gttcgcgcat
    28441 atccggcgtg ttttgcattc cacgtcgtag ctgcttttcg gtggcgaaat agagttcgcg
    28501 caacagactt tgcttccagc tattccacag cgtttcgttg gtggcgcaaa tgtcagccac
    28561 tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg caaactgctt
    28621 gatcacttcc gggtcctgaa tatcgcggcg ctgagctgtc accgacatca gcaagtgctg
    28681 gcgaaccagc cacgccacca gctgtgtttc gcgcgagtta agcccgtgga gttcggcaaa
    28741 atgcaccacg tcttgtgcac cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc
    28801 gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc agacgtccac
    28861 acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt tcagcatcac
    28921 gcgaatagta tgttcatcca ccgtgtaggc gtggaacagg tcaaactgca tctgcccgac
    28981 gatatgcgac cattggggca tatacgcgcc gagcacgcta tgacgatgca tcggtaatag
    29041 tccgcgacgc accgcaccag ggtgacgcag aatgctcaaa aacagttttc gcgcttccgg
    29101 aatattacac agcggttgtt gcaaatggcg acgggcgtgg cgtaactggc gcagcgtggt
    29161 agagtaaatg ccagtgatcg cactgttgcg caccatggtg tagaacatac gcaagatggc
    29221 ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg
    29281 aaactcatcg tcgattggac gtggtttttc gtcggcggga agggcgagga tggcttcatc
    29341 gaacagttgc agcagcatct ggttgagttc gctgacgcgg cgtgtaacgc ggaagtaatc
    29401 cttcatcatc cgctcgaccg gttcgttacc ttcaccactg taattcaaac gctgggcgac
    29461 gctaagctgg cgatcgaata acaggcgatt atcgtaacgg ctgacgacaa gatgcagggc
    29521 aaagcgaata cgccacaata tatgcagaca ttcgtttaat tccgctcgct ccgctgaggt
    29581 taagaagcca aacccgacca tttcatccaa cgatgttgcg ccaaaatgac ggcgggccac
    29641 ccattgcagc gtgtggatat cgcgcaagcc gccggggcta cttttgatgt ctggttcaag
    29701 gttgtagctg gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc
    29761 gtagaacttg tcggaaggcc agaatccttc gctgaaaata tgtttttgca gttcgaggaa
    29821 cagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga cggttaaatc
    29881 cgataaccct tccagcatgc actcttcaag cgtgcgcacg ctatgaccga cttccagttt
    29941 cacatcccat aatagtgtta acagttcgcc aactttctgt gcctgatcat ccgggagttt
    30001 cttacggctt aagatcagca aatcgatgtc ggaaagtgga tgcagctcgc cacgaccgta
    30061 gccaccgacg gcgactaatg ccaggtcggc aatctggctg aatcccgcgt caatccataa
    30121 ccgttgcagg agctggtcga taaactcggt acgcgcctca atcaactctt ctgcagagat
    30181 tccattgtca aaggcatcac ccagccaacg ctggaaagta tcaatatggg cttttatccc
    30241 acagacggtt aattcatcac ggggccaggc acatggattt tgcggttgac cgggaagggt
    30301 ggggagagcg gtatttgcgt actgttctgg aagggtattc atcgtgcgcc accca
//
LOCUS       DAOTRK010000051        30216 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.051, whole genome shotgun sequence.
ACCESSION   DAOTRK010000051 DAOTRK010000000
VERSION     DAOTRK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.051"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..755)
                     /locus_tag="QIE41_002158"
     CDS             complement(<1..755)
                     /locus_tag="QIE41_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS3531041.1"
                     /translation="MNDRLCFEVHDNQGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWG"
     gene            complement(1166..2350)
                     /locus_tag="QIE41_002159"
     CDS             complement(1166..2350)
                     /locus_tag="QIE41_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDS3531042.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLQNTFEA
                     ITREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(2650..2744)
                     /locus_tag="QIE41_002160"
     tRNA            complement(2650..2744)
                     /locus_tag="QIE41_002160"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2708..2710),aa:Sec,seq:tca)
     gene            3037..4419
                     /locus_tag="QIE41_002161"
     CDS             3037..4419
                     /locus_tag="QIE41_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDS3531043.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFLGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQNVQN"
     gene            4429..6747
                     /gene="yicI"
                     /locus_tag="QIE41_002162"
     CDS             4429..6747
                     /gene="yicI"
                     /locus_tag="QIE41_002162"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDS3531044.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWK
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTESG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            7042..8595
                     /locus_tag="QIE41_002163"
     CDS             7042..8595
                     /locus_tag="QIE41_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDS3531045.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLEQWRITVEKRAGAGVMLDSKNITDLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            8622..9092
                     /locus_tag="QIE41_002164"
     CDS             8622..9092
                     /locus_tag="QIE41_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDS3531046.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIAQLLVETSPQKIIDLFSQYAQSDVY"
     gene            9110..9424
                     /locus_tag="QIE41_002165"
     CDS             9110..9424
                     /locus_tag="QIE41_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDS3531047.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAAEISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            9449..10540
                     /locus_tag="QIE41_002166"
     CDS             9449..10540
                     /locus_tag="QIE41_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDS3531048.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            10552..11403
                     /locus_tag="QIE41_002167"
     CDS             10552..11403
                     /locus_tag="QIE41_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDS3531049.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            11484..12344
                     /locus_tag="QIE41_002168"
     CDS             11484..12344
                     /locus_tag="QIE41_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDS3531050.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            12392..13324
                     /locus_tag="QIE41_002169"
     CDS             12392..13324
                     /locus_tag="QIE41_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDS3531051.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGVALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYMEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            13305..13985
                     /locus_tag="QIE41_002170"
     CDS             13305..13985
                     /locus_tag="QIE41_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDS3531052.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGAAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRAVKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNHFLEPGGRFPAIEEDEPVKWLL
                     CSEYAVLR"
     gene            complement(14028..15737)
                     /locus_tag="QIE41_002171"
     CDS             complement(14028..15737)
                     /locus_tag="QIE41_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDS3531053.1"
                     /translation="MKFIGKLLLYIFIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANIPLKPTVSGQLHAVNATKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(15858..17249)
                     /gene="xanP"
                     /locus_tag="QIE41_002172"
     CDS             complement(15858..17249)
                     /gene="xanP"
                     /locus_tag="QIE41_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDS3531054.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            17529..18734
                     /gene="gltS"
                     /locus_tag="QIE41_002173"
     CDS             17529..18734
                     /gene="gltS"
                     /locus_tag="QIE41_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDS3531055.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(18768..20849)
                     /gene="recG"
                     /locus_tag="QIE41_002174"
     CDS             complement(18768..20849)
                     /gene="recG"
                     /locus_tag="QIE41_002174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDS3531056.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRNDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(20855..21544)
                     /gene="trmH"
                     /locus_tag="QIE41_002175"
     CDS             complement(20855..21544)
                     /gene="trmH"
                     /locus_tag="QIE41_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDS3531057.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(21551..23659)
                     /gene="spoT"
                     /locus_tag="QIE41_002176"
     CDS             complement(21551..23659)
                     /gene="spoT"
                     /locus_tag="QIE41_002176"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDS3531058.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(23678..23953)
                     /gene="rpoZ"
                     /locus_tag="QIE41_002177"
     CDS             complement(23678..23953)
                     /gene="rpoZ"
                     /locus_tag="QIE41_002177"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDS3531059.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(24008..24631)
                     /gene="gmk"
                     /locus_tag="QIE41_002178"
     CDS             complement(24008..24631)
                     /gene="gmk"
                     /locus_tag="QIE41_002178"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDS3531060.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            24889..26571
                     /gene="ligB"
                     /locus_tag="QIE41_002179"
     CDS             24889..26571
                     /gene="ligB"
                     /locus_tag="QIE41_002179"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDS3531061.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGNETRDVMMPPLNGAVIHPVAHTGVRK
                     MADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTASGPLANSTLQGEIFLKREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELTTAGFTLTQTYTRAVKNADEVARVRNAWWKAKLPFVT
                     DGVVVRAAKEPESRYWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRVALNASDERS
                     WSQLLLSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(26568..27185)
                     /locus_tag="QIE41_002180"
     CDS             complement(26568..27185)
                     /locus_tag="QIE41_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDS3531062.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(27476..28300)
                     /gene="dinD"
                     /locus_tag="QIE41_002181"
     CDS             complement(27476..28300)
                     /gene="dinD"
                     /locus_tag="QIE41_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDS3531063.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQISIKQLENSVKITEKK"
     gene            complement(28521..29384)
                     /locus_tag="QIE41_002182"
     CDS             complement(28521..29384)
                     /locus_tag="QIE41_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDS3531064.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            29511..>30216
                     /gene="rph"
                     /locus_tag="QIE41_002183"
     CDS             29511..>30216
                     /gene="rph"
                     /locus_tag="QIE41_002183"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDS3531065.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAA"
BASE COUNT         7147 a         7331 c         8013 g         7725 t
ORIGIN      
        1 ccccataatg gtgagtatgt tgcatagtaa tcttctgccg tatccagaga gccggagaag
       61 tcatgccaca cagcaagtgg aaatggatgc agatttccgc acagcatttc tgcgatgtaa
      121 gtgtttgtgg caaagccatg gcgaaaagct gtggcggcct taacatgttc cccgttcaga
      181 aaatgcagga gaccgagttc ataccagtaa ggtgaaaatt catcagcatg ctctttcagt
      241 acactgaagg ctcgctcatg atcgccggtg cgtaacagct ctgaacccaa caaccagcga
      301 gccccctgat tatcttcagg attgtatgcc agtattttat cagtgagcat aaccgcatcc
      361 tgatggcgct gtaagtgcac atttgcgaga attgctgcat aaagcgcgcg cagataaggc
      421 cggttttcgg gatgcatcca gattatttca ccacagaaac tttccgggat gatacgatta
      481 cctgccgcca gtcctttaag agcggcgttc agtgccttac gcggggcatt ctgttccaga
      541 aatgcatatg ccagatgagc gtgtatatca ataaagtccg gctcccgttg agcaaggcgt
      601 cggagtttgt tgatgtaact cgtctcgctg atttcatcag cgtcgtatgc atcaagcact
      661 tcttcaaact cccccagcaa tgggccaaac caggtatccg gaaatacgaa ataaccctgg
      721 ttatcgtgca cttcaaagca taatctatcg ttcattttct gagcctgtaa aacatgtggt
      781 gatatagtct gagagcataa aagttgagat tctgtgtatc aagtcctgtc ttggcactga
      841 atgaatattg cttacaaagc cccttcatgc tttccttgag aacaataatt ttatctttat
      901 gtgaatctca ttttttataa tgatgtcaga tgattgaaat gctaaattat atctgaatca
      961 acttttcctt tgctaaaggt atcgaaataa ctgcaaggct gtattttgta attgccaatt
     1021 taaaaatgtc aatttgcttt taattatgtt tttgtaataa gtttgaagac gcaaaaaata
     1081 atataataac aaaaatcctc cagccttgta ggccagagga taaaatactt aattgaggaa
     1141 aacatcaggg aaaagtattt ttgacttaac tttcccggct ggagagtgaa tcaatgtaat
     1201 cagcgtacca ttgcatcatt tcacggcggc catcgagata cagggcatga ttataggtac
     1261 ccctgatgga atttttatcc acatgagcta actgcatttc aatccaggcg gaattaaagc
     1321 cttgttcatg cagaattgta ctcatggtgt gtctgaaacc gtgccctgtg agtcttccct
     1381 tatatccaag taattcaatt acttggttaa tgctttcctt actaattggt ttgcggggat
     1441 catttctacc gataaagact aggggatgat gttttgagat tggttcaagc tgcttaaata
     1501 gcataatgac ctgctcagag agcggcacaa tatgaggtcg cttcatcttc attacttctg
     1561 ccgggatctc ccacaatttt gtctcaaaat cgatatcttc ccagcgtgca aaacgcagtt
     1621 cttgtgttcg tacgccggtt aacataatga tctgagtagc tgtcttagtg attatacttc
     1681 cggtataacc cgccagatcg ttgaggaagt ggggaagttc attagcagta agaaagggaa
     1741 aatgtacttt tttcggtgta gctaaagcac tggcaagatc gggggctggg ttatagtctg
     1801 cccggccagt aacaattgca tagcggaaca cctcaccaca gcgctggcgg accttgcgca
     1861 ttttctccag agctccacgt ttttccattt tacgcagtgc ttccagtaac tccattggtt
     1921 tgatttcggc aatagggcgc ttgccaatgt agggaaaaat gtctttttca aaggtatcaa
     1981 tgatttcatc acgatagcgt aatgaccacc gatcggcttt tgactggtgc cattcgcgag
     2041 taatagcctc gaacgtattt tgcagactca tttgctgcgc gattttttct tccttttttg
     2101 cctcccctgg atcgcctcca gcagcgagga gtttcctggc ttcactgcgt ttctcgcgag
     2161 catcggctag cgttacatcg gggtatacgc caatagaaag catcttttct ttaccagcaa
     2221 agcggtactt catgcgccag tatttagaac cattagtgtt aatcagtaga tacataccgc
     2281 cgccatcagc aagcttatag ggtttgtctt tgggttttgc ggttttcact ttgatatcgg
     2341 ttaatgccat gacatcctcc ggcttttgat gaattcatgt tcctgagggg gatttctgcc
     2401 agtaactaac ctgatacccc caatacaccc cttgtataac aaagatgttg ttaaaagccg
     2461 ttgttatata tcatattttt cctattatgg gggtgctggt atttcaatag gaaataacac
     2521 cccctaaagt acccccaaaa gaaacttgat acggggtgaa gtaggttgac ctctggatac
     2581 aaataaatac tgatatacaa gggattagaa gagatttatt gacattggga gagttcagaa
     2641 gaggtaaact ggcggaagat cacaggagtc gaacctgccc gggaccgctg gcggccccaa
     2701 ctggatttga agtccagccg cctcaccgga gacgacgatc ttccgcgcct cgattgctac
     2761 atggaggcgg ggcgcattat agctacttcc ttgagtttct acatccccca gatcagtttt
     2821 ttccgcctaa tacacgcagc gtattatcac ggaagcatta taattcatat aaaacccata
     2881 ttttacaatg atatatgagc ggaattaacg ctaccacgat cacataaaac aagaatcata
     2941 aattaataac cagatatcgg aatattcgct ctcacaggga tggtttacaa aatgcgttat
     3001 cggtctcagc acccctatag catcaaggaa aagcagatga agagtgaagt gttgtccgtt
     3061 aaagagaaaa ttggttatgg catgggagac gccgccagcc acattatttt cgataacgta
     3121 atgttatata tgatgttctt ttataccgat atttttggca ttcctgccgg atttgtcgga
     3181 actatgtttt tggtcgctcg tgcactggat gcgatttccg atccttgcat ggggttgttg
     3241 gccgatcgaa cgcgctctcg ctggggtaaa tttcgtccgt gggtactgtt tggcgcactg
     3301 ccatttggga tcgtctgtgt actggcctat agcacgccag atctcagtat gaacggaaaa
     3361 atgatttatg cagcaattac ttacacccta cttaccttac tttataccat cgttaatatc
     3421 ccttactgcg cattgggtgg tgtaatcacc aatgacccga ctcagcgtat ctcgctgcaa
     3481 tcctggcgtt ttgtgctggc gaccgcggga ggcatgctct ctactgtgct gatgatgccg
     3541 ctggttaatt taattggtgg tgataataaa ccactcggtt tcctgggcgg tatcgcggtc
     3601 ctttccgtgg tggcattcat gatgctggca ttttgtttct ttaccactaa agaacgcgtt
     3661 gaagcaccac ctacaacgac gtctatgcgg gaagatttac gtgatatctg gcaaaacgat
     3721 cagtggcgga ttgtcggttt actgaccatt ttcaatattc tggcggtgtg cgtacgcggc
     3781 ggggcgatga tgtattacgt cacatggatt ttgggtacgc cggaagtgtt tgtcgctttt
     3841 cttaccactt attgcgtggg taacctgatt ggttccgcac tggcgaaacc gctgaccgac
     3901 tggaaatgta aagtcactat cttctggtgg acgaacgccc tgctggcagt gattagcctc
     3961 gcgatgttct ttgttcctat gcaggccagc atcactatgt ttgtcttcat cttcgtgatt
     4021 ggtgtgttgc atcaactggt gacacctatc cagtgggtaa tgatgtccga taccgtcgac
     4081 tacggcgagt ggtgcaatgg taaacgcctg accgggatca gttttgctgg cacgctgttt
     4141 gtgctcaagc tggggttggc cttcggcggc gctcttatcg gctggatgct ggcttatggc
     4201 ggatatgatg cggcagaaaa agcgcagaac agcgccacga ttagcatcat tatcgcgcta
     4261 ttcacgattg ttccggcgat ctgttatttg ctgagcgcga ttatcgctaa acgctactac
     4321 tcactcacga cgcacaatct gaaaaccgtt atggaacagc tggctcaggg caaacgccgt
     4381 tgccagcaac aattcacctc tcaaaatgtg cagaactaag gaacggcaat gaaaattagc
     4441 gacggaaact ggttgattca acctggcctc aatttgattc acccgcttca ggtgttcgag
     4501 gttgaacagc agggtaatga aatggtggtc tatgctgccc ctcgtgatgt gcgtgaacgt
     4561 acctggcagc ttgatacgcc tttatttacg ctgcgctttt tctccccaca ggaaggtatt
     4621 gtcggtgtac ggattgagca ttttcagggg gcgctgaata acggtcctca ttacccgctc
     4681 aatattttgc aggacgtgaa ggtcacaatc gaaaacacag aacgttacgc tgagtttaaa
     4741 agtggcaact taagtgcgcg tgtcagcaaa ggtgagttct ggtcactgga ttttctgcgt
     4801 aacggcgaac gtattaccgg tagtcaggtg aaaaataatg gctacgtgca ggacacgaat
     4861 aatcaacgaa attatatgtt tgagcggctg gatcttggcg ttggcgaaac agtttacggt
     4921 ctgggagagc gctttactgc gctggtgcgc aatggccaga cggtagagac ctggaaccgg
     4981 gacggcggca caagtactga acaggcgtat aaaaatatcc cgttctatat gactaaccgt
     5041 ggctatgggg tactggtcaa tcatcctcaa tgcgtctctt ttgaagtggg atcggagaaa
     5101 gtctccaaag tgcagttcag cgttgagagt gaatatctcg aatactttgt tatcgacggc
     5161 ccgacgccga aagcggtact tgatcgttat acccgtttta ctggtcgtcc ggcgctgccg
     5221 cccgcgtggt ccttcggcct gtggctaacc acttcattta ccaccaacta cgacgaagcg
     5281 acggtaaaca gttttatcga tggtatggcg gaacgcaatc tgccactgca tgttttccac
     5341 tttgactgtt tctggatgaa agccttccag tggtgcgatt ttgagtggga cccgctgact
     5401 ttcccggacc cggaagggat gatccgccgc ctgaaagcga aagggctaaa aatctgcgtc
     5461 tggattaacc cctatatcgg gcaaaaatcc cctgtcttta aagagttaca agagaaaggc
     5521 tatttactca aacgcccgga cggttcgtta tggcagtggg ataaatggca gccaggtctg
     5581 gcgatttatg actttaccaa tccggatgcc tgcaaatggt acgccgacaa actgaaaggt
     5641 ctggtcgcga tgggcgttga ttgctttaag accgactttg gcgaacgtat tccaaccgat
     5701 gttcagtggt ttgacggttc cgatccgcag aaaatgcata accattatgc gtacatctac
     5761 aacgaactgg tgtggaaagt gctcaaggaa accgttggcg aggaagaagc cgtcttgttt
     5821 gctcgctcgg cctccgttgg tgcgcagaaa ttcccggtac actggggtgg tgactgttac
     5881 gctaactacg aatcaatggc ggaaagcctg cgcggtggtt tgtctattgg cctttcaggt
     5941 tttggtttct ggagccacga tatcggcggc tttgagaata ccgcgccggc gcacgtttac
     6001 aaacgctggt gcgcgtttgg tttgctctcc agccatagcc gtttacacgg cagcaaatct
     6061 tatcgtgtgc cgtgggccta cgatgatgag tcctgtgatg tggtgcgctt cttcacgcaa
     6121 ctgaaatgcc gcatgatgcc gtatctgtat cgtgaagctg ctcgtgcgaa cgcgcggggt
     6181 acgccgatga tgcgggccat gatgatggag tttccggacg atccggcttg tgattacctt
     6241 gaccgtcaat acatgttagg cgacaacgtg atggttgcgc ctgtgttcac tgaatcgggc
     6301 gatgtgcagt tctacttgcc tgaaggtcgc tggacacacc tgtggcacaa cgatgaactc
     6361 gatggtagtc gctggcataa acagcagcac agcttcctga gtctgcccgt ttatgtgcgt
     6421 gataacaccc tactggcgct gggcaacaac gagcaacgtc ccgattacgc gtggcacgaa
     6481 ggcacggcat tccagctctt taatctacaa gacgggcatg aagccatctg tgaagtgccc
     6541 gctgctgacg gttccgttct tttcaccctg aaagcggcgc gtactggcaa cacgattact
     6601 gtgactggtg cgggcgaggc gaagaactgg acactgtgct tgcgcaatat tgtgaaagta
     6661 aatggtctgc aaggcggttc gcaggctgaa agtgagcagg gactggtggt gactcctcaa
     6721 gggaatgcgc tgacaattac gttgtaatct attgaagaat gccggatacg aatggttttg
     6781 tgtcgtatcc ggtaaataat ctcacatctg gtaataaata tttcttccat cgtatttgcg
     6841 agcatgatca cgattattca tcacgaaaat gtgatctgtg ccgaaaaata tctattaacc
     6901 gccccgtttt gttttataca tgtccggact acggacagat tttagtattg ttgtgaaatt
     6961 attctgcttt atatttaccc tcagagatgg caactgaagc tcttccacat ggggcttctt
     7021 aatctgtgat aagggtaaaa aatgcaaatg attacctcgc gacaaaacag attattacga
     7081 ttccttttac cacgaaggga atatacgact attgttacga ttgccggcta tttaaatgtt
     7141 tcggaaaaaa ccattcaacg tgatttacgt ttacttgagc aatggctgga acaatggcga
     7201 ataaccgttg agaagcgtgc tggcgcgggt gtgatgttag actcgaagaa tattactgat
     7261 ttgctgcatc ttgatcattt gttgggagca gaatgtgaag agattgatgg tgtaatgaat
     7321 aatgccaggc gcgttaaaat agcgtcgcag ttattaagtg aaacaccgaa tgaaacgtcg
     7381 atcagtaaat tgtcagagcg ctattttatc agtggagcct ctattgttaa tgacctgaga
     7441 gtgattgagt cctggcttgc gccgttgggg ttatcattga tccgcagccc aagtggtacg
     7501 catattgaag gtagtgaagg gcaagtccga caggcaatgg cattactgat taacggcatt
     7561 attaaccata atgagccgca aggtgtcgtg tattcacgtc tggatcccgg aagttataaa
     7621 gcattagtcc attattttgg agaagaagag gtgttatttg tccagtcatt gttactggat
     7681 atggaaaatg aattaagttg gtctttggga gagccttatt acgttaacat ttttactcac
     7741 atccttatta tgatgtatcg caacacgcac gggaatgcgt tatccagaga agaagatcaa
     7801 accaggcaat atgatgaaaa tatctttaat gttgccagtc agatgattca taagatagaa
     7861 caacgaattg cacatacatt gcccgatgat gaagtctggt ttatttatca atatatcatt
     7921 tcatcaggtg tggcgattga tggacaaaaa gatgtgagca ttatttcaca tatgcaggcc
     7981 agcaatgaag cgcgtctgat tacctggcgt ttaattacgg tattcagtga catcgtggac
     8041 tgcgatttta gtgaagacag cgcattatat gatggcttgt tggttcatat taagccgctg
     8101 attaaccgac taaattatcg cattcatatc cgtaatccat tgctggaaga tattaaagca
     8161 gaattagcgg atgtctggcg gttgacgcaa tatgtggtga atcaggtatt taaaacctgg
     8221 ggtgagaatg cagtgagcga ggatgaagta ggttacctga ccgttcattt tcaggctgcg
     8281 atggagcggc aaattgcccg taaacgtgta ttactggtct gttcaaccgg aatcggaact
     8341 tcgcatctac tgaaaagccg tattctgcga gcatttcctg aatggacgat tgttgatgtt
     8401 atttcagcag cgaatttatc acaggttttg cctgacaata tcgaactgat tatttcgacc
     8461 attaatttgc ctacagtcac tatgccggtc gcttatgtta ccgctttttt taatgatgcc
     8521 gatattaagc gggtcactga aatggtgatt acggaaaaat tacatcatgc gacatctcgg
     8581 gtcgttgaaa tttaatgtca gaattttatg aggtatgacg aatggacata acaaaaatcc
     8641 tgaatacgaa tcgcgttatt ttagatatgc aagcgacgaa taaagcggag gcgattgaag
     8701 aattaactaa tcttctcaaa aaagatggtg cgatcgattg tcgggaaacc tttattaaag
     8761 atgtctggca gcgcgaagcg gaaggctcga ctggttttga aaatcatatt gcgatcccgc
     8821 atggtaaatc ttctgcggta attaatacca cgctggctat cgggcgtacc cggcaggata
     8881 ttccctggga aacactggat ggcagtagcg tacgctgtat tattttattt gcggtacgtc
     8941 ttgaagatca aaataccact catatccgtt tactttccca ggtcgcgggc gcgcttgcag
     9001 atgatgacat catagcgcaa ttgctggttg agacgagccc gcaaaaaatc attgatctct
     9061 ttagtcagta tgcacaatcc gatgtctact aaataacagg gagtatatca tgaatattgt
     9121 ctgtgttgcc gcctgcacgg cgggtattgc tcacacctat attgctcgtg aaaagttaat
     9181 taaaggggca aaagcactgg gccacaccat taaagttgaa acccagggaa ccattggtac
     9241 agaaaacgaa ttacaggcgg cagagatctc tgctgctgac gtcgtgattc ttgctgtgga
     9301 tgtgaaaata aaaggagaag aacgttttac caataaacgc attgtgcgag taaagacaga
     9361 gatcgtcatt aaatcgcccg ttcaatttct tgaaaaggtc gaaaaatcac tgggaaataa
     9421 atgatcctta tactggaggg atcttaatat gaaagaaaat aaaattccgg tttcacagga
     9481 aattaaaaaa catttattaa ccggcatctc gtggatgata ccccttattg tggccgccgg
     9541 gatatgtatt gcgttggggc aagttcttgg cggtacagat gttggtgaaa aaacaggaac
     9601 aatcccgtgg atgcttaatc agattggcgg ctggggaatg gggctgattg tgccgctgat
     9661 tagcgcggca attgcctatt ctattgccga tcgtcccgga tttgccccgg gcctgattgt
     9721 cggctttctc tgtgggcaaa tccataccgg atttattggc ggtatgttgg gtggtttcct
     9781 ggtgggctac accattttgc tacttaaacg ctatattcgc ctgccgcagt cgatgcaagg
     9841 actcatgcca atcatggtgt taccggtatt gagcaccatt attggtggtc tgttaatgat
     9901 gacgctgatt ggtaaaccca tcgcctggtt acaagaagca ctgattcatt tgctcgagtc
     9961 gatgcagggc ggatcgcgct tcctgatggg ggccatactt ggcgcgatgg caaccttcga
    10021 tttcggtggt ccggtcaaca aaaccatgtc gctgttttcc gatggcttgc tggtcagtgg
    10081 cgtctacgga ccagaagccg tgaaatttgt cggctcaatc atccctccgt ttggcatcac
    10141 actttcgttc ttactgacac gccataaata cacccgtgcg gagcgcgagg cgctgaaagc
    10201 cgcgtttccg atggggatct gcatgatcac tgaaggagtc attccgatcg ccgcgcgtga
    10261 cctgttgcga gtagtgggat cttgtgtggt ggcatcggcg gtcgcgggcg gcttaatcat
    10321 ggtgtggggc gtggagagtc cggttccaca tggcggcatg tttgttgtgc ctctgttcac
    10381 ccatcctctg ctgttttgcc tggcgctggg tataggcacg gtgatttgtg gcgtgatgct
    10441 ttcactctgg aagaaaccgg taacggaacg cgacgaagag tttgacgaac tgagcgatca
    10501 aaagctgaaa gatgaagaga tcactttcac cctcgaataa ccggaggccc tatgtttgcc
    10561 gatatgaaaa gtatggtgat gaaagcctgg catgaacatt acgccttgct ggcgatcaac
    10621 tgtatgaatc tggaaagtgc tcacgcagct atccgcgtgg cggagaagaa tcgcgctccg
    10681 atcattctca atctctatca ggggcatctg gcgcattttc ctgcacccgt cgcggcggca
    10741 gtggttaaaa cgctcgcgga atctgcctca gtaccggtcg ctctggcgct tgatcatggc
    10801 aaaaatccgg actgtattcg tcaggcgttt cgtgctggtt tcagcggatt aatgattgat
    10861 gcttctgctt ttcccctgga ggaaaacgtg cgccagacca gggcggtggt ggaactcgcc
    10921 gccagcgcgc agctctgcgt ggaaggcgaa ctgggccatc tggcagatgc gccgcgttac
    10981 gaccaggccg ccaatgcaga cctgatgacg caacccactg atgtggaacc gtttatcgcg
    11041 cagacaggca ttgatctact ggccgtctcg gtaggcacgg cgcacggcat gtacgcgcct
    11101 ggtgttgtac ctgctattga ttttcagcgt ctggcggaga ttttccactg ctcgagcgtg
    11161 cctctggcat tacatggcgg tagcggtacg ccgttcgatc agctccagct ctgtacgacg
    11221 ctgggggtag caaaaatcaa cgtgggggcg gccatttttg aacgggggaa atccgcgctc
    11281 ctgcataccc tctgtcacga tatatccatt gaactggtgg acgcgctgaa agctatggaa
    11341 ctggccttcg aagaagcgat tacaccgtat ttacaggcga gcggctctat aggcaaagcc
    11401 tgattcattt tctgtcatgg cctttatgcc agtgcaataa cgcaccggca gggcggacta
    11461 cacgctgaaa ctggagattt aacatgttag tttcgatgaa agaattactg caacctaccc
    11521 gccagcacgg ctttgccatt ggcgcattta atgtggcgga taactgtttc cttcgcgcag
    11581 tcgtggatga agcagaagcg acaaatactc cgacgattat tgccatccat cccagcgaac
    11641 atgattttgt gggcgatgcc tttttcgctc acgttcgcga aattacccag cgtagtccgg
    11701 tcccgtttgt actgcacctc gatcatggtg cctctgtaga gcatgttttg cgtgcgattc
    11761 gctgcggatt tacctcagta atgatcgacg gttcgctgct gccgtatgaa gaaaacgtgg
    11821 cgctgaccgc agaagtggtt cgcctcgctc acgcggttgg agtgtcggtt gagggtgaac
    11881 tgggaaccat cggacaaaca ggaacgtcgg tagaaggtgg tgtttcacaa gtgacctaca
    11941 cagatccggc gcaggccgcc gattttattg ctcgtacagg tgccgacaca ctggctgtgg
    12001 cgattggtac agcgcatggc atctatccaa aagggatgca gccgaagctg caaatggaca
    12061 ttttgcgcga cattgccggg cgcattgata ttccattggt gttgcacggt ggttcggcaa
    12121 atccggacgc ggagattgct gaatcagtta cgctcggcgt tggcaaaatc aatatctcca
    12181 gcgatatgaa atacgcctat ttccagaaag tgcgtgaaat tctcgccaaa gagagctggt
    12241 gggatccgaa cgtcatctat ccggatgcga ttaacgcggc gcgggaagtc attcgccaca
    12301 aaatgaaact gttcggttcg ctcggtaaag catcactgta ctaagccaga agggcgggat
    12361 gacccgcctt ttacgcaaca aggaacgaga tatgcactat ctggggttgg atattggtgg
    12421 gaccaaaatc gccgccgtcg tcatggatgc gcatggctgg gagattcgcc gttaccgctg
    12481 cccgacgcaa aagtcgacat atcaacaatt tgtctcatgc gttgtggcgc ttatcgagca
    12541 gattaggcgg gacgttcaac gaccgatgct gacaggggtc gccttacccg gcagtatctc
    12601 gccactcact ggcctgatta aaaacgcgaa tattcaggtg attaacggtc atgcgttaca
    12661 ggctgatttg cagcaattgc ttgggcaacc ggtggtgata gccaatgatg gtaactgttt
    12721 cgcgctatca gaagcttgcg acggtgccgg gcaagattat gacgtagtat ttggtattac
    12781 gcttggtaca ggctgcggcg gtggcattgc catcaagcaa cgaccgttta taggggcctg
    12841 gggaaatgct gccgaatgcg gtcatatcac gttgccaggc tatatggagc aggaagatgg
    12901 tccatcagtc agttgctatt gcggcaaaca caactgcgtg gagtcgtttg tttccggcag
    12961 cggttttagt gaacgctatc aacagatgac tggtaacttg ctcactcctg cggcgattgt
    13021 caccctggca caacgtggtg atgcttgtgc catgcagcag gtggcacgtt ttcgccaaca
    13081 gcttgcccgc acgctggcaa ccatcgttaa cgttgttgac cctggcgtga ttgtcatcgg
    13141 cggcgggctt tcgaatgtgg aactgcttat caccgatctg aacgcagaag tcgctccact
    13201 ggttttcacc gatcaattca ccactcccat tgtaaaagca cagcacggcg acagtagcgg
    13261 aatgcgtggc gctgcctggc ttgctatgcg caacggagaa gccaatgaaa cgttcacaaa
    13321 ttaattatgt tattgataaa gcacatgcca ttgcgcagac cttccgcgtt tgtttgccag
    13381 agttcgccta ttttacggtg gatgactggc tgcagcggga acgggataac tggcaagaag
    13441 tggtcgactt acagctcggt tgggatatca ccgattttgg tcgtggtgat ttcaaccaaa
    13501 cgggactaac actgctaacg atgcgcaacg gcgcgctggg ggcggcggca tatccaaaac
    13561 cgtatgctga gaaaatgttg cagatccaac aagatcagca gactccctgg cattttcaca
    13621 cccacaaaat ggaagatatc ctcaatcggg gcggtggaga cttatgcatg cagttggcct
    13681 gggccaacga agccaatctc tgtgatgatc agcgggttat taccgtgagt gttgatggtc
    13741 agcggcgtgc cgtgaaacct ggtgaaacgc tggtgctaaa gccggggcag ggcatttgcc
    13801 tgccgccgcg tctgtatcat cgcttctggg cggaaaaagc gtttgttctc ggctgggaaa
    13861 tttcgatggt aaatgatgac caacatgata accattttct tgagccggga ggacgttttc
    13921 cggcgataga agaagatgaa ccggtgaaat ggttattgtg tagtgagtat gctgttttgc
    13981 ggtaatagcg gggacgctaa cgttgatatc cgttagcgcc cgcgtattta ctgcaccggc
    14041 tccgtcgatg taacttcgcc actggaaacg acgcctgcat tcatggtttg agtcacttgc
    14101 tgctttgtgg cgttcacggc atgcaattgc ccgctaaccg taggtttcag cgggatattg
    14161 gcctgaatat cgccgctggc ggtaagctga atattgccgt cgccagtcaa cggtaattca
    14221 ggccatcccc actgttgcaa aatattcacc ggcacaccgc gtccattcag gctgatatgg
    14281 gtctgacgtt gtggcgtttg tgaaacactg gcggtggctt ccagaatgcc tttttcagta
    14341 aatgcactca gttcgctgat attcaccgtg ctgctgttgg cggtcagcgc cagcgatggg
    14401 cgacgaacat caacacgatt aaatgtcgcg gcggcggcat tcagattcgc cgaaccactc
    14461 cagacgcccc atttatgatc ggtaaccagc gtcaggttgg caccgtaacc atcgagcgcg
    14521 gtgagctgcc acgggaagtc agggtcgata tcaatgatca gattgcggct ggcgctaaat
    14581 ctcttcagtt gcaggctgtt taaccaaccg ggtgttgttt ccatccacaa ctgttgccag
    14641 ttttttggca aggtatattc cagtccggca attgccgcat catcgaggat cagcgttttc
    14701 ccgtcgcgtt gccagtttcc tgacgttctg accatacccc cttcccagcg gctggtgaac
    14761 tggcgcagcg ctacgccctg cggggaaaat tccgcgttta taatcgggtc aaataaatgc
    14821 agcgaaccat aaatgaactc gctggcgttc atcgacagtt tgccttcctg tgtctgccag
    14881 tcatctttac tgaaggtcat gttgcgcaag ctgagatcga ggtcggtcac cgcccagtcc
    14941 ggaccttgca aacgagcatc gatcacttcc aggcgaccaa tctgcaacga cgggacagag
    15001 cgtaatggcg caaagaagtc ggttagcgat ttttcgcttt gtagacggat atccgccatg
    15061 cgcagatttt ccacttgcca gctgccgtcg gcgttacgct gcgcgtttcc ggttaatgtc
    15121 ccgcgggcga tgtcggcacc caggttagtc agcgtaacgc gatcgttatc aatactgcct
    15181 tcaatcagta cattggtggc aggaacatca ttgagcgaaa gcgatccggc actaaactga
    15241 atctgcgcct tcgtacccag cactttaccg gcttctggtg accacggaac cacgccgcca
    15301 tttacccgct gcgcgctcag tttccattcg ctattcgggc tattaaacgc catatcacgc
    15361 agttgcagac gatcggcttt gaacggtagc ggcgcggtct ggtcggtgag attcagcgtc
    15421 ccgttttcca gcaggatggt atcgacatgg cgtggttcgg tcagttgccg actgcttagc
    15481 gcaatgtcga cactttttgc caccagggtc gcgggctggc catcacggcc gaacgtgacg
    15541 ttctccagca cgatatgaga tggcgcggaa aaacggtgat ccatcgcccc gaaggccaga
    15601 tgatagtcgc tattctcgga aacccatgcg ctgatatgtt ctgctcccca gcgggtttgc
    15661 agaagaaaat agaggccagc gatcaccact aacagagcga tgaaaatgta gagaagcagc
    15721 ttcccaataa atttcatggt cttccatccc gtgaaatgca cataagggag ttatgcacga
    15781 tttacgcgca atcctcaagg cgggaatggt gaaagaggtg agactgcggc ggtaattatc
    15841 ttaccgccgc cagtgaatta ctgtttttct ggtgggaaaa tcagattcag cacgatggca
    15901 gtaataccgc ccgcggcgat cccggaggag agcaggtttt tcagccactc aggggcaaac
    15961 tgcaaaatca gcggctgctg agagacgccc agaccaaccg ccagcgacag cgcgataatc
    16021 agaatcgccc gacggttcag cggctcacga gacacaatgc gcacaccgga ggcggcgatg
    16081 gtgccaaaca ttacaagtgt tgcgccgccc agaaccggtt ctggaatatg ttgtacaaaa
    16141 ccgctcactg ccgggaacag acccagcacg atcaacatca gcgcgacgac aaaaccgaca
    16201 tagcggctgg caaccccggt caactggatc actccgttgt tctgcccgaa gcaggagttc
    16261 gggaaggtgt taaacaccgc cgaaacaaac gagttcaggc cgtttgccag cacgccgcct
    16321 ttcaggcgtt tcatgtacag tggaccggac actggctgtt cggaaacgtc agaggtcgcc
    16381 gtgatatcgc caatggtttc cagtgaagtg atcataaaga ccagcatcag cggcagcagc
    16441 agactccatt caatgccaag accgtaatag agcggcgttg gcaccataat cagttcttgc
    16501 gtcatcggtt cgttgctttc tggcaacatg cccataaacc acgccagcgc atatccggcc
    16561 gccatcgcaa tcaccagtga ggccacgcgt aagtaagggt tacgttgacg gttaagcagg
    16621 ataattaagg ctaagaccac gcctgccagc agcagatttt tcggtgcgcc gaaggtgtta
    16681 tcgctcatgg ctgcgtaacc gccgccaatg gaagtcagcc caacctgaat tagcgacagg
    16741 ccgataatca tcaccacaac gccagaaacc agcggcgtaa taatgcggcg cgccagatgc
    16801 agaacgcggg agatcaccat ctcggtgcaa cttgccagca tcaacgtgcc gaacaaagcc
    16861 gccatcatgg taggaacatc agcaccaccg gtcttcagcg ctgtgccgcc cataatcagc
    16921 ggggcaacaa agttgaagct ggtgccctga atagacaaca acccggagcc aaccggaccc
    16981 caggccttaa tttgaataat cgatgccaca ccggaggcaa acagcgacat actaataatg
    17041 tgttgcgtgt cttgtgccgg taaacccagc gcctggcaga ttaatagcgc tggcgtgatc
    17101 accgcaacga acatcgccag cagatgctga caggcggcaa acagagtttg aggaagcggc
    17161 ggacgatctt caagacggta aatcagttcg ctgttttgag tctgcgcaac cggttgcgca
    17221 ttttctgact cgagggtgga aacagacatc gaaaacaatc ccgtggtagc aaagcgggca
    17281 ttttagctga ccgaaagaga aaagcaaacg tttgccacat cctcatctaa atatttaaat
    17341 gagataaatg cactttttta tataactttt ggtatttttc tgcttaaaat ccatgccatt
    17401 tgttcattaa cagctgagaa tttgacccag gaaagcataa ctcgataccc gtcatagttc
    17461 atgaagttgc cactgcgcga tgaagtatga cgagtatgaa agagtgatgc ggatacaaag
    17521 gagtaactat gtttcatctc gatactttag caacgcttgt tgccgcaacg ctgacgttgc
    17581 tgctcgggcg taagttggtc cattccgtct cctttttgaa gaaatacacc ataccggaac
    17641 ctgttgcggg tggtttgttg gtggcgctgg cgctactagt actgaaaaaa agcatgggct
    17701 gggaagtcaa ctttgatatg tccctgcgcg atccgttaat gctggctttc ttcgccacca
    17761 ttggcctgaa cgccaacatt gccagtttgc gtgccggtgg gcgtgtggtt ggcatcttct
    17821 tgattgtggt tgttggtctg ttggtgatgc aaaatgccat tggcattggt atggctagcc
    17881 tgttagggct tgatccgctg atggggctgt tggccggttc tattacgctt tccggcggtc
    17941 acggtacggg cgctgcgtgg agtaaattgt tcattgaacg ttatggcttc accaatgcga
    18001 cggaagtggc gatggcctgt gcaacgttcg gtctggtgct gggcggcttg attggcggtc
    18061 cggtggcgcg ctatctggtg aaacactcca ccacgccgaa cggtattccg gatgaccagg
    18121 aagtcccgac ggcgtttgaa aagccggatg tgggacgcat gatcacctcg ttggtgctga
    18181 ttgaaactat cgcgctgatt gctatctgcc tgacggtggg gaaaattgtt gcgcaacttt
    18241 tggctggcac tgcttttgaa ctgccgacct tcgtctgtgt cctgtttgtt ggcgtgattc
    18301 tgagcaacgg tctgtcaatg atgggctttt accgcgtctt tgagcgtgcg gtatccgtgc
    18361 tgggtaacgt aagcctgtcg ttgttcctgg cgatggcgct gatggggctg aaactgtggg
    18421 agctggcttc gctggcgctg ccgatgctgg cgattctggt ggtacagacc atcttcatgg
    18481 cgttgtatgc catcttcgtt acctggcgca tgatgggcaa aaactacgat gcggcagtgc
    18541 tggctgcggg tcattgtggt tttggcctcg gtgcaacgcc aacggcaatc gccaacatgc
    18601 aggcgatcac tgaacgtttt ggcccgtcgc acatggcgtt cctggtagtg ccgatggtcg
    18661 gtgcgttctt tattgatatc gttaacgcgc tggtgattaa gctgtattta atgttgccga
    18721 tttttgcggg ctaaccgatg aagcggcggt cttctcaccg ccgcctttta cgcattcgag
    18781 taacgttccg tctccggcat ccagcgttct atcagggctt ttgcttgttg tgggtaacgt
    18841 tcgtgaatat gtcgcgccag gcgctgaact tccgggatca tcgcctgatc gcgcagtaaa
    18901 tccgccactt taaactcagc attacccgtc tgacgcgtac ctaacagttc acccggtccg
    18961 cgaatctcta agtctttctg tgcaatcaca aaaccgtcgt tgctgtcgcg cagcacttgc
    19021 agccgaattt gcgcggtttt ggagagcggc gttttgtaga gcagcacgca gtgagaagcc
    19081 accgcaccac gacctacacg cccgcgaagc tggtgcaact gcgccagacc cagacgctcc
    19141 gggttttcga taatcatcag gctggcgtta ggcacatcaa cgccgacttc aataacggta
    19201 gtggcaacca gcaggtgtaa ctcgccttgt ttaaatgatg ccatgacggc ctgcttctcg
    19261 gctggtttca tccgtccgtg taccagacca acattcaact ctggtagcgc cagtttcaac
    19321 tcttcccagg tagcttccgc cgcctgcgct tccagtaact ctgactcttc aatgagtgtg
    19381 cacacccagt acgcctgacg cccttcagtc atacaggcgt ggcgcacgcg gtcgataatg
    19441 tcgttacgac gcgtatccgg gattgccact gtcgtcactg gcgtgcggcc tggcggcagc
    19501 tcatctatca ccgaggtatc gagatcggca tacgcggtca tcgccagcgt gcgggggatc
    19561 ggcgtcgcgg tcatgatcaa ctgatgcgga tggaagccct gctgctggcc tttctcccat
    19621 aacgccagac gctgatgcac gccaaaacga tgctgttcgt cgataatcac cagcgccaga
    19681 ccgttaaact gcacctgttc ctggaagatg gcgtgtgtac cgacaatcat ctgcacctga
    19741 ccgctggcga tggcttcctg ctgtgccagt cgcgctttac ctttctgctt accggcgagc
    19801 cagcccactt cgataccgag cggtgcaaac cagttgcgga agttattcgc gtgctgctcg
    19861 gcgagtaatt cggttggtgc catcaatgcc acctgtttgc cgtgggcaat cgcacgcaac
    19921 gcggcgaggg cggcgaccag cgttttaccg gaacctacat cgccctgcac caggcgcatc
    19981 atcggcgcat ccagcgccat atcgcgctcg atctccgcca ccacgcgtgc ctgtgcgccc
    20041 gttggcttga acggtaaggc tgcgaggagt ttatttttca gcgcgtcatt ggcgctaagc
    20101 ggctgggcat ggaaacgttg tgcaccagcg cgtaacgcca acatgctgag gttgtgcgca
    20161 agcagttctt ccagaatcag acggcgttgt gctggatgct gcccggtttc gagatcgcta
    20221 agctgtagcg tcggcggcgg gcggtgcaaa gtgcgcaacg cttccggtag cgtcatcatt
    20281 ccttgtgaca gttccggcgg caggagttct tcaatggcgc aggtgtcgag cagatccagc
    20341 gcctggtcgg ttaatttacg cagcgtggcc tgctttacgc cttcggttgt tgggtaaacc
    20401 ggcgtaagcg tttcctgcaa ctctggcgta ctgagatcgc cctgcacgcg gtattccggg
    20461 tggatcatct ccgcgccata tttaccgcgc tttgcttctc cataggccag tacgcggcgg
    20521 cccgtcgcca ggctattttt cattgccgcg ttgaagttga aaaagcgcat ggtgagaatg
    20581 ccggaaccgt cgctgatctg gcaggtcatc atccgccgac cgccgaagga gatattgcag
    20641 ttcagcactt cgccttccac cgtggcataa acgcccggta gtagttctcc gatggggtag
    20701 agatgggtgc gatcttcgta gcgcagggga aggtgtaaga gtagatcctg cacggtatgc
    20761 aggttgattt tcgccagctt gttactaagc gctgcaccaa cgcccgttag ggaactgagc
    20821 gggacagcat ctaacaggcg acctttcatg gtacttaccc tgcagcttgc atagtggccc
    20881 accagtcggc atcggcttca atttcgccct gctgattgac gtgggggtaa ggcaggcctt
    20941 tgcgttttgc gactttcgcc agcaccggat agccgccttc aaacagcagg cgttgttgct
    21001 ctgcttccgg caacatgctg ttttcacgca ggtacatgcc cgcattttgc cgctggcgct
    21061 gggcttcgta aagaatgagg gctgaggcaa cggaaacatt cagcgattgc accatgccaa
    21121 tcatcggaat gatgatgtcc cgatccgcca gggccaacgc ttcctgcgtg atgcctgttt
    21181 tctcctgtcc catcaaaata caggtcgggc gagtgtaatc aatttcgcgg aaatcgacag
    21241 cgttatcaga aagatgggtt gccagaatct gcatgccctg gcctttcaga tgagcgacgg
    21301 catcgccaat ggtgcggtgc gttttcacct gtacccagct gttactaccc gcagctgccg
    21361 aagccatggt gcgcatccgg ctaccaggcc agacagcatg aacttcatgt acaccaacgg
    21421 catctgcggt acgaataatc gcagaaacgt tatgaggttt gtggacctgc tccatgcaga
    21481 cggtcagatc aggctgccgc ctggcgagca tttcgcagat acgtgcataa cgtgttgggt
    21541 tcataaaaca ttaatttcgg tttcgggtga ctttaatcac gtctggcatc acgcggattt
    21601 tgcgcatgat attcgccaga tgcacacggt cacgggcggt cagacgaata aaggcgctgt
    21661 agacgcgacc atctttctct tccgtattca aactttgaat attcgaggtg gtggtgttaa
    21721 ttgccgctgt caggtttgcc agcgcgccct gatgattgaa catctccacc ttgatttcgg
    21781 tgatgaactc ctgcgccgtc tctttatccc attccacagc cataaacttc tctggctctt
    21841 tctggtagcc acggatgtta cggcaggatt catggtggat caccagacct ttaccggggc
    21901 tgacgtgggc gataatcggg tcgccaggaa tagggcggca gcatttcgca aaggtgatca
    21961 gcacgccatc ggcaccttta atgggcagat gtccgtgact ttgggttgcc ggtggaatgg
    22021 aggcgtcccc atgttgcaga tttttcgcga ccaccacgct cattgcgtta ccaaggccga
    22081 tttcagccag cagatcgtca agcgttgcca gcttcatgcg gtccagctcg cgctgaatat
    22141 tttcctgcgg gatttcattg agcttgcggc tgccacccaa cgcatggttg agcagacgac
    22201 ggcccaggct tacagaatca tcacgcttga ggtttttcag caattgacga attttggcgc
    22261 gcgctttcga gctaacgaca aagttcagcc acgcggcatt cgggcgagcg cccggtgcgg
    22321 taataatttc aacggtttga ccgctggtca gcggctgcga cagcgggtaa ggctggcggt
    22381 cgacacgcgc gcccacgcag gcatgaccga tatcggtatg caccgcataa gcgaagtcga
    22441 cgggcgttgc accggcaggc agctctacaa tgcgcccttc cggtgtgaaa acgtaaattt
    22501 catccgggaa gagatcggat ttaacgctct cgataaattc aaacgagcta ccggcactct
    22561 gttgcagctc cagcaggctt tgcatccagc gctgggcgcg gatttgtgcg gtagtactgg
    22621 tttcgccgtg ctctttataa gcccagtgcg cggcaacacc catctctgcc atctgatcca
    22681 tatcttcggt acggatctgg acctcaaccg gcacgccgtg cgggccaatc atcgaggtgt
    22741 gcaaagactg atagccgttc gcttttggaa tggcgatgta gtctttcacg cgacccggac
    22801 gcggcttgta caggctatgc atttgcccga gcacgcgata acaggtgtcg gaatcattga
    22861 cgatcacgcg gaaggcgtag atgtccatga ttgagtgaaa acgctgctct ttgagcacca
    22921 ttttgcagta gatcgaataa agatgctttt cgcgaccact gacgcggcac ggtattcccg
    22981 cttcctgcaa acgcccttcg atttcagaaa gaatcttctg gatcatctct ttacggttgc
    23041 cgcgcgcggc tttcaccact tctttgatta cgcgataacg gttgggatac agcgcctcaa
    23101 aacccagctc ttcgagttcg gttttaatgt ggtggatacc taaacggtgc gccagcgggc
    23161 tgtaaatttc gagtgtttca cgggcgatgc ggcgacgttt gtccgggcga agtgagccca
    23221 gcgtgcgcat gttgtgggta cggtcggcaa gtttgatgag aatgacgcgg atatcctgca
    23281 ccatcgccat aatcatcttg cgaaagtttt cggcctgcgc ctctttctta tcgcggaact
    23341 tgagcttatc aagtttcgac accccctcta ccagctcggc gacgctttta ccaaaaagct
    23401 gttccatatc ctgataggtg gcgggtgtat cttcaatcac gtcatgcagc agcgccgcca
    23461 ttagcgtttc atagtcgagt ttcatctcgg ccagaatgca ggcaaccgct accgggtgcg
    23521 tgatataggg ttcaccgctt gaacgtgttt gcccctcgtg agcatcacgt gcaacgagat
    23581 acgcctgccg cagacgcttg atttggtctt ccggcaggta ggtttgaatc agttgattca
    23641 ggctttcaaa cagatacaag ggcgacccgc tttgtgatta acgacgacct tcagcaatag
    23701 cggtaacggc ttgtaattca gcggcttcct gctcttgctg ttcctggcgt tcgcgaacgt
    23761 cgaggatctg gttgttgatc agaccttctt cgatttcgcg cagcgcgatt acagtggttt
    23821 tatcgttttc ttccggtacc agcggatcct ttccgcctac ctgcatctga cgagcgcgac
    23881 gcgcggcgac cagtaccagg tcaaaacggt taccaatttt ctctacagcg tcctgaacag
    23941 ttacgcgtgc catacttaaa aagctccaca ggtgaagaaa tgactgggca tgatactgaa
    24001 atcaggttca gtctgccaac aatttgctga ttaaagcgtc atgacgctgc ttttggcggc
    24061 tcatgcgcag acgttcggcg cgaataatgg tcttcaaatc ggtcaacgcg gtatcgaagt
    24121 catcattcac aatcaggtaa tcatattcgg cgtaatggct catttctgca acagcttgcg
    24181 ccatacgctt tgcaatgacc tcttcgctgt cctgaccgcg accgcgtaga cggcggtcca
    24241 gttcaatttt ggacggcggt aaaataaaga tactccgcgc gtgcggcatc ttctggcgaa
    24301 tttgctgcgc accctgccag tcgatatcga gaaaaacatc gacaccggtc gccaggactt
    24361 gctcaatggc ctcacgcgaa gtgccatagt aattaccaaa aacttctgcg tgttcgagga
    24421 acgcatctct gctaatcatt tctttaaatt catcatgatt aacaaagaaa taatgttcac
    24481 cgtggacttc accaggacgc ggttggcgtg tggtgtgtga aacagaaacc tgggtgtcat
    24541 acaacggttg ggtttttaat aaagcctgaa tcaggctgga tttacccgcg ccactggggg
    24601 cagaaacaat ataaagcgtg ccttgagcca tgagtatctt tcgtatgtga ttagcgaaat
    24661 aaagcctaca tacgagctta ttatacacgg cgcgggcatg tgacgtagcc cttgctcaca
    24721 ctttttgcgc aaagttgttg cgttttgcgt agctgcttcc gcatttgcct gtcattcact
    24781 gcaataaaga cgattctcag gaagcgtcct cgcagactcc gtttttgcca ctcggctttg
    24841 ctttcatcac gttgtatatc tgcctgacta tctacagcgg aggtgatgat gaaagtatgg
    24901 atggcgatat taataagtat cttgtgctgg caatcatcgg cgtgggcagt ctgtccggcc
    24961 tggtcgccag ccagagcaca ggaagaaatt tcccgcctgc aacagcaaat aaaacagtgg
    25021 gacgatgact actggaaaga agggaaaagt gaggtggaag acggtattta cgatcagtta
    25081 agcgcccgtc ttacgcaatg gcaacgctgt tttgggaacg agacccgcga tgtgatgatg
    25141 ccgcctttaa acggtgcggt tatacatccg gttgcccata cgggtgtacg caaaatggcg
    25201 gataaaatcg cattaagtct gtggatgcgc gaacgtagcg atctttgggt gcagccaaaa
    25261 gttgatggcg ttgcggtaac cctggtttat cgggacggga aactgaacaa agcaatcagt
    25321 cgcggcaatg gcctgaaagg cgaggactgg acgcagaaag ttcgcttaat ttccgctgtg
    25381 ccgcaaaccg ctagcgggcc tttagccaac agtacgcttc agggggaaat atttctcaaa
    25441 cgcgaggggc atatccaaca acaaatgggg ggaataaatg cccgcgcaaa agttgctggt
    25501 ctgatgatgc gccaggacga tagcgacacg ctgaattctc tgggcgtttt tgtctgggca
    25561 tggccggatg gaccgcagtt aatgaccgat cgtttaaaag agctgactac cgctggtttt
    25621 actctaacgc agacgtatac ccgtgcggtg aaaaatgctg atgaagttgc gcgcgtacgc
    25681 aatgcgtggt ggaaagcgaa attacccttc gtcaccgatg gcgtggttgt gcgagcggcg
    25741 aaagaacccg aatcccgcta ttggctacca ggccaggcag aatggttggt ggcctggaaa
    25801 tatcaaccgg tagctcaggt tgccgaagtg aaggcgattc agtttgcggt gggtaagagc
    25861 ggtaaaatat cggtggttgc gtcactcgca cctgtcatgc ttgatgataa aaaagtccag
    25921 cgggtgaata ttggttccgt caggcgctgg caggagtggg atattgcgcc tggtgatcag
    25981 atcctcgtca gccttgccgg tcagggtatt cctcgcattg atgatgtggt gtggcgcggt
    26041 gcagaacgca caaaaccgac accgccagaa aaccgcttta actcgttgac ctgctacttt
    26101 gcttctgatg tttgtcagga acagttcatt tcacgcttag tctggctggg gtcaaaacag
    26161 gttcttgggc tggatggcat tggtgaggcc ggctggcgcg cgctgcatca gactcatcgc
    26221 tttgagcata tcttttcctg gcttttatta acgccagagc aattacagaa cacgccgggg
    26281 atcgcgaaaa gtaaaagtgc gcagttatgg catcagttta atctggctcg taagcagcct
    26341 tttactcgct gggtgatggc aatgggaata ccgctaaccc gggtggcgct taatgccagt
    26401 gatgaacggt cctggtcgca acttttattg agcacggagc agttctggca gcagctgccg
    26461 gggactggat cggggcgcgc cagacaggtt attgaatgga aggaaaatgc gcaaatcaag
    26521 aagctgggca gttggctggc tgcccagcag atcacaggtt ttgaacctta gtgtccttcg
    26581 tggctgtagt aaaagaccgg taatcccagc ttaaggcgta gcgccagtaa acgggcaaaa
    26641 aagccgaata ccagggtgga gataattacc acatcatgat tagaaacata gtgttgcagc
    26701 gcgatgtaca gcacggcgga ggcaaatgag acaccggcat acaattcttt ctggaatacc
    26761 agcgggatgc gtttacagaa catatcgcgc aaaacgccgc caaatacgcc ggtcgtcaca
    26821 gctgcgacaa cggcaatgat cgggccgtgt cccatatcca gtgcaacctg tgcgccaatg
    26881 atagaaaaga ccaccagtcc gagcgcatcg agcaccagaa acactttgcg caggtaaggc
    26941 attacaggag caacgatagt ggtgagtact gcggcggtag cgacaataat cacatattcc
    27001 gggtgtttga cccagccgag cggatagtgg cccagcagaa tatcgcgcac tgaccctccg
    27061 ccaattgccg tggcggtcgc aataataatt acgccaaatg tatccatgcg ccgtcgtccg
    27121 gccgccagcg cccccgtcat ggcttcggca gtaatgccaa ccaaatacaa aatgtgtaac
    27181 agcatggtta tctccctaaa aatctgtctg gcagggtagc gatttgtacg cattgtcacg
    27241 attgagattt tttaaggtga gaggaaattg ataattttta gttatgtgaa atcgttgttt
    27301 tctgtagcgt agatgcattc ttctgacttc ttgtctgaga aaatacataa gaaaaagtag
    27361 gttaaaactg aggggtggaa aagaaaggtg caatgttagc cattgtgttc accgcacatg
    27421 gactttggct tacgcatcgg gtatctcaat caacaaaaag agtttttagt ttcggctatt
    27481 ttttctctgt aatttttaca ctgttttcca actgcttgat gctgatctgc ggggtcggca
    27541 actcctcagg catggttccg ccaagttcct gaatggtttg cctcaccttg cgacccacgt
    27601 caaagtgtgt ggtgttagct tgctgttttg aattcacgcc atcccgcttg agtttttctt
    27661 ctgtttgggt agctcgaaag agattagccg ccagttctgt tgaacccata tgatccagga
    27721 tcttctgact ctttttcagc cccttccgct gatggatggc tttctgatct aatccaccat
    27781 acagcccccg gtaaccatga ttctgaaata tggcgaaatc gatagctgtc gctaccccag
    27841 cttgctgcgc tgcctcaact aattgtttgt tatgttcttt caactcattt ctcagaaaaa
    27901 gacgcttttc gtcttcacga agttgcctga aagcttcatc gtcagccagc tcctgtcgtc
    27961 gggtctggat agcaaaataa gtttgccctg ccgcaataac cggtttcgca gggtcgccgt
    28021 tttgtactac caggtaacag gcatagcggg agagtacgat gtcttttaat gctcttttgg
    28081 catttgagcc aattttgacc atatcgagga tctcctcgaa atggtcatca attgtgtgac
    28141 cactattcag acaagcttct ttggcgcgcg ttaatacagg tataaagtga cgatactctg
    28201 aataacccag tagtttccca agttgtcttg ctgaccattg ttctgctccg tttgcattaa
    28261 tcagctttat ctcttcaaaa ggttgatgat gttcgttcat tgcccactcc atgtgatatc
    28321 gattgcaagg aaagtaagtc tacaactgtg tttatataca gttgtgagta tctcacgttc
    28381 tcactgcgct gggcaaaaaa acatcgcaaa atctggagca ggcacgcaaa acagaattga
    28441 atgttgcggg gaagaaaaat gccgctctgt tttagtggcg gcatttgtga aggcggaaaa
    28501 gtaagaattg gcgttacgag ttactcgata ttctggatct gctcgcgcat ctgctcaatc
    28561 aacactttca gctcaatcgc ggagtttgtc acttcggcat tgatagactt cgacgcaaga
    28621 gtgttcgact cgcggttgaa ttcctgcatc ataaagtcca gacgacgacc aacggcttct
    28681 tttttcttca gaatgttgta ggtctctttg acatgcgctt ctaggcggtc cagttcttcg
    28741 gcaacgtcaa ttcgttgtgc cagcagaacc aactcctgct ccagacggtt gttttccaac
    28801 tgaacctgag catcttccag cttcgcgacc agacgctcac gctgccattg caggatttcc
    28861 ggcatatggg cgcggacttt gaccacttcg gcggtgacgc cttccagacg ctgctcgatc
    28921 aatgctttca gcgcctgacc ttcggtttcg cgcgcgacga taaagtcgtc cagcgtacca
    28981 tccaacgccg cgagaatttc agcggcaatc gcgtcgagat cctgctcctg ggctgccatc
    29041 acgcccggcc agcgtagaat atcaaccggg ttgatttccc cttcgtcact ctgcattttt
    29101 acccagttcg cggcagttac cagctgttta gccagttttt cgttgaggat cagctcacct
    29161 tgtgcgctaa catctggttc atagcgcagg gtacattcca ctttaccgcg cgtcaggcga
    29221 gaacgaatac gctcgcgaac gacaggttca aggctacgga actgctccgg cagacgaaag
    29281 taagtttcca gataacgctg gtttaccgag cgcatttccc aggttgcgct cccccattca
    29341 cccttgattt cacgccgggc gtaggcggtc atactgcgga tcatagacgt tcctgtttat
    29401 aaaaggagag gtggaaggat tatagccatc gatgccttgt caggatagga ataaccgccg
    29461 gaagtccgta taatgcgcag ccacatttgt ttcaagccgg agatttcaat atgcgtccag
    29521 caggccgtag caataatcag gtgcgtcccg ttaccctgac tcgtaactat acaaaacatg
    29581 cagaaggctc ggtgctggtc gaatttggcg ataccaaagt attgtgtacc gcctctattg
    29641 aagaaggcgt gccgcgcttc ctgaaaggtc agggccaggg ctggatcacc gcagagtacg
    29701 gcatgctgcc acgttctacc cacacccgta acgcgcgtga agcggcgaaa ggtaagcagg
    29761 gtggacgcac catggaaatc cagcgtctga tcgcccgtgc tcttcgtgcg gcagtagatt
    29821 tgaaagcgct gggtgagttc actattacgc tggactgcga cgtgcttcag gctgatggtg
    29881 gcacgcgtac cgcttcgatt acgggtgcct gcgtggcgct ggcagatgcg ctacagaagc
    29941 tggtggaaaa cggcaagctg aaaaccaacc cgatgaaagg gatggtagcc gcagtttctg
    30001 tcgggattgt gaacggcgaa gcggtttgcg atctggaata cgttgaagac tctgccgctg
    30061 aaacggacat gaacgtggtg atgactgaag acgggcgcat cattgaagtg caggggacgg
    30121 cagaaggcga gccgttcacc catgaagagc ttctcacctt gttggcgctg gcccgagggg
    30181 gaatcgaatc cattgtagcg acgcagaagg cggcgc
//
LOCUS       DAOTRK010000052        30171 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.052, whole genome shotgun sequence.
ACCESSION   DAOTRK010000052 DAOTRK010000000
VERSION     DAOTRK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.052"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(175..1224)
                     /gene="yahK"
                     /locus_tag="QIE41_002184"
     CDS             complement(175..1224)
                     /gene="yahK"
                     /locus_tag="QIE41_002184"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDS3531066.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQVGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1311..2267)
                     /locus_tag="QIE41_002185"
     CDS             complement(1311..2267)
                     /locus_tag="QIE41_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3531067.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVSLGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(2264..3235)
                     /locus_tag="QIE41_002186"
     CDS             complement(2264..3235)
                     /locus_tag="QIE41_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3531068.1"
                     /translation="MAELKKRHEFWLALLIVGLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(3228..4712)
                     /locus_tag="QIE41_002187"
     CDS             complement(3228..4712)
                     /locus_tag="QIE41_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDS3531069.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRHLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(4761..5744)
                     /locus_tag="QIE41_002188"
     CDS             complement(4761..5744)
                     /locus_tag="QIE41_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS3531070.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMENLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(5998..6408)
                     /locus_tag="QIE41_002189"
     CDS             complement(5998..6408)
                     /locus_tag="QIE41_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDS3531071.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNVFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQVEGSYSPEMDVIFEEDTLIDIV"
     gene            complement(6707..8089)
                     /locus_tag="QIE41_002190"
     CDS             complement(6707..8089)
                     /locus_tag="QIE41_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDS3531072.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQS
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(8099..9049)
                     /locus_tag="QIE41_002191"
     CDS             complement(8099..9049)
                     /locus_tag="QIE41_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDS3531073.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWRFVEDGGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(9145..10563)
                     /locus_tag="QIE41_002192"
     CDS             complement(9145..10563)
                     /locus_tag="QIE41_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDS3531074.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(10563..12110)
                     /gene="fdrA"
                     /locus_tag="QIE41_002193"
     CDS             complement(10563..12110)
                     /gene="fdrA"
                     /locus_tag="QIE41_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDS3531075.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQTGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINAAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFCGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLELHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(12100..12963)
                     /locus_tag="QIE41_002194"
     CDS             complement(12100..12963)
                     /locus_tag="QIE41_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDS3531076.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSHCLRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNT
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(13003..13608)
                     /locus_tag="QIE41_002195"
     CDS             complement(13003..13608)
                     /locus_tag="QIE41_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDS3531077.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGIDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDQICSNPFLISCLTGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            13866..14363
                     /locus_tag="QIE41_002196"
     CDS             13866..14363
                     /locus_tag="QIE41_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDS3531078.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSTFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            14455..15387
                     /locus_tag="QIE41_002197"
     CDS             14455..15387
                     /locus_tag="QIE41_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3531079.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(15429..16517)
                     /gene="pdeL"
                     /locus_tag="QIE41_002198"
     CDS             complement(15429..16517)
                     /gene="pdeL"
                     /locus_tag="QIE41_002198"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDS3531080.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(17394..19427)
                     /gene="betT"
                     /locus_tag="QIE41_002199"
     CDS             complement(17394..19427)
                     /gene="betT"
                     /locus_tag="QIE41_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDS3531081.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            19556..20143
                     /gene="betI"
                     /locus_tag="QIE41_002200"
     CDS             19556..20143
                     /gene="betI"
                     /locus_tag="QIE41_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDS3531082.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVDGNFDETQVS
                     NAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFHRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            20157..21629
                     /gene="betB"
                     /locus_tag="QIE41_002201"
     CDS             20157..21629
                     /gene="betB"
                     /locus_tag="QIE41_002201"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDS3531083.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNNELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQNNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            21643..23313
                     /gene="betA"
                     /locus_tag="QIE41_002202"
     CDS             21643..23313
                     /gene="betA"
                     /locus_tag="QIE41_002202"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDS3531084.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNITVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTARYFVANGMPVRVKK"
     gene            23524..24192
                     /locus_tag="QIE41_002203"
     CDS             23524..24192
                     /locus_tag="QIE41_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531085.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKMRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(24435..25130)
                     /locus_tag="QIE41_002204"
     CDS             complement(24435..25130)
                     /locus_tag="QIE41_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDS3531086.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNVVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(25123..26550)
                     /locus_tag="QIE41_002205"
     CDS             complement(25123..26550)
                     /locus_tag="QIE41_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDS3531087.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNVRKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(26561..27280)
                     /locus_tag="QIE41_002206"
     CDS             complement(26561..27280)
                     /locus_tag="QIE41_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDS3531088.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLMLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(27808..28662)
                     /gene="rclR"
                     /locus_tag="QIE41_002207"
     CDS             complement(27808..28662)
                     /gene="rclR"
                     /locus_tag="QIE41_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDS3531089.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            28889..>30171
                     /locus_tag="QIE41_002208"
     CDS             28889..>30171
                     /locus_tag="QIE41_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDS3531090.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENASIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPS"
BASE COUNT         7650 a         7968 c         7202 g         7351 t
ORIGIN      
        1 aaatcgctca taactaacgt gtgaagtatt gtgtactgga gggagttaat ttaacaattt
       61 gaatttacgt tataggtttt actgctactt tgatattatt tcgctctcct taactaaaaa
      121 gttaagtgga gagcgaagtt aatatgttaa accacagggt atttattaat tttttcagtc
      181 tgttagtgtg cgattatcga taacaaaacg atatttcaca tctcctcgca gcattcgctc
      241 ataggcttca ttaatttgat cggcccgaat catctctata tcagccacga tgccatgttc
      301 ggcgcaaaaa tcgagcatct cctgagtttc tggaatgccg ccaatcatag aaccggctat
      361 cgcacggcgt ttcatgatca ggttgaaaac ttccggcgat ttatgcggtg tcgcaggcgc
      421 accaaccagc gtcatggtgc catcacgctt cagcaaggtg gtaaaatcgt cgagattatg
      481 tggcgcagct actgtattca aaatgaaatc gaaactcttc acatgagccg ccatctcatc
      541 ggcattgcgt gagttaacaa cttcatcggc ccccagggct tttgccgctt cgcgttttga
      601 ctcagaagtg gtaaatgcca ccacatgcgc ccccatcgcg tgggccagct taatccccat
      661 atgtcccaga ccgccgatgc cgaccacgcc cactttttta cccggaccga cctgccagtg
      721 acgtagcggc gaatacgtgg tgatccctgc acacaacaaa ggtgccaccg ccgccagctg
      781 ctcttgcggg tgacgaatac gcagaacata tcgctcatga acgacgatct gttgtgagta
      841 gccgcccaga gtatggcccg gttcgtccgg cgtcggcgag ttataggtgc cggtcatgtg
      901 atcacagtag ttttccagcc cgtcttcaca ctcttcgcaa tgtttacaac tgtcgacaat
      961 acagccgaca ccgaccagat cgcccggcgc atatttttct acctgatcac caacagctac
     1021 cacgcgccct acaatttcat ggcccggcac gcagggataa accgtccccg cccactcgga
     1081 acggacctgg tggatatcgg aatggcagac gccacagtaa gcgatttcaa ttttgacatc
     1141 atgcggtccc ggttcacgcc gggtgatatc catcggttca agtggttgtt tagcggaata
     1201 tgcaccaaca gctttgatct tcattgtgtt tactcctgat tagctatgtg tatttggcga
     1261 tgcctgatat gtcatcgcgg caaaacgcgt ccattgaata tagccaatat ttaaaccacc
     1321 cgatccagcc ccaacatgcc gcgagcctga atgaaacaga gcagcaacgt tccccataag
     1381 gccatcgtca ggtaggggct tataccgaga atattgaagc cgctttcaat aatttgcaga
     1441 agaaacagcg ccagcaccat accaattatc cgcccgctgc cgccgtccgg attaaccccg
     1501 ccgagtactg ccgcgaggat tgacaccagc aagtacgatt cgccataaga ggccttagcc
     1561 gagttgagtt tcgacatcat caaaaatgcc gctaccgcac aaagcaaggc ggaaatgaca
     1621 tacacccaaa tcagcacccg acgagtgtta atgccggagt aaagtgttgc tcgttcgtta
     1681 gaaccaatca gataaatgga tttaccgagc ggcgttttct ccagcaatat ccacagccct
     1741 agcgacactg cggtaaacaa ccacatcggt agcggaatac cgaaccactg tgcatgattc
     1801 aaccacagca cccatgaggg ataattggca atggcgctac cgcctgtcac cagaatattt
     1861 acccctttga gcaacgtcat catcccaagc gttgcaagaa ttggcgacac tcgaatacca
     1921 gcaatcaata cgccgttaca taaaccgatt atcaccgccg cgccagcgcc cgccagcagc
     1981 gtagcgacaa ccgtagcaat acccggtgga tactgagtcg ccacccacgc catcaccagt
     2041 gaacaggcgt tagcagtggc tataattgac aagttaatgc caccgcaaag catggtcacc
     2101 gccatagcca acgccagaat gcccagcacg ggcatttgcg aagcgacgga ctggaaatta
     2161 cttatcgacc agaaaatctc cggcatagta atgctgaagg ctgcaacagt tagcaccagc
     2221 aggccgatca agtaaaactc aacgttgtta cgccacgatt ttttcatagc agactccggt
     2281 tctgatgctg actccacgcc gtggcactaa tgctggcaac gatgatgatg ccggtgatca
     2341 atgtttgcca gtaagacgag actcccaata aatttagccc gttttgcatc actgccaaca
     2401 acaccacgcc gagcaaagta ccggttaacg tgccgcgccc gccgagcaaa ctagtgccgc
     2461 caagcaccac cgcagccagt actgtcagct cataacccag aagagaatcg ggggcgacag
     2521 tcatcaccgt ccacgactgc actacacccg cagcaccaga catcaatccc atatagccgt
     2581 agacgaaaag ttgcagtttc agcacgctaa agccgatgcg actggcggat tcacggttcc
     2641 cgccaagggc ataaattttg cgccctacac tggtgaaatt catgataaac gctgtcagca
     2701 gcaccaccgt aatcatggcg atcagcggca gaccaaggcc atagtcatag ccatcagcat
     2761 cggtgtactt gaacaacatt accccctgct caaaccacgg cggaaagtcg tacagccaca
     2821 cacctttact caaccataac agcaggccat aaaaaatatt cagcgtcgag atagtgatga
     2881 tgatagaagg cacccgtagg cgattaacca gtaaggcgtt aattaagcca agtaaaatgc
     2941 cgatgccgcc cgccagggca aacgcgacag caaagttgcc accaattttt tgcagcagca
     3001 gcaccatgcc gtattgcgca atgatggtca ttgccggaaa cgaaatatca atcccaccgg
     3061 aaattaacac cacaaacaaa ccacaggcga gaatagtcaa catggcatag ttattggcga
     3121 gatcgtacaa attaccgaat gtcagaaact cgtcgctgcg ccaggcaagg ccgacaaaca
     3181 gccccacaat cagtaatgcc agccagaatt catgacgttt tttcagttca gccatttacc
     3241 acctccgcaa tcctggcctg agaactgctg tcaggcagga agctatgggt gatctgacct
     3301 ttttgcatta ccaaaatccg gtggctttga taccacgctt cttcaatttc atcgcagatc
     3361 atcaataccg caatgccatg agccgccaga tcgctgataa tgccgtatat tcctgctttg
     3421 ttggcgatat caacaccgac tgtcgggcta tcgagaatca acagtcgtgg cccaatcgcc
     3481 agccatttgg cgatcgatac tcgctgcgcg ttacccccag aaagcgtatt caccgggaga
     3541 tgaggatcag aaactttgat ggtcaatgcc ttgattagcc tgtccaccag gtcacgcact
     3601 ttagcttcac tcagaaagcc gaagcgatct tttaccttgt gaaaaacagt gctaatgatg
     3661 ttgtcttcaa tagattgcgc catcaccaaa ccgcgcgaca tgcgatcttc cgaaacataa
     3721 ccaataccgt ggcggatggc gtcctggtta ctatgcagtg taaccggctg accgttgatg
     3781 aggatctcgc cagcatccgg tcgggtcata ccaaacagac tcagacataa ttccgtgcgc
     3841 cccgcaccaa gcaggccaac aatcgacacc acttcgcccg cttccacccg caagttaata
     3901 ttttgatatt ccccatgacg acttaaatga cgcacctcca gcaccggagt gctggatttt
     3961 ccctgccaca actctcgcac ctgatattcg aactcctggc cggtcatcag aaaactgagt
     4021 tgttttgtgg tcatctctgc ggccggaaag gtcccgacca gctcaccatc tttcaacact
     4081 gaaatacggt cggaaacttc catcacctct tccagacgat gactgacgaa gaccacgcag
     4141 atcccgcgtt cccgcaattg atgcaccacc tgtaataacc cttgcacttc ctgatgggtc
     4201 agcgaagcgg ttggttcatc catcacgatc aaccgtgcgt cctgcgccag tgcacggcaa
     4261 attgccacca gctgacagcg ggcaattgaa agctcagaaa cgagcgtatc cagaggcaat
     4321 gtgacgttaa tactggtcat tgccgcctgc gccacctcac gcagacgacg tcggttaaca
     4381 aacagaccgt ggtgatagtg attcacggcg atattctccc agacactcag gttaggaaat
     4441 aaagagaggt cctgataaat cacctgaatc ccctgcgcca ccgaagcggc gggtgtcagc
     4501 tttgaccagt ttttcccacc aagcgtaatt tccgctccct catccgggcg ataaacaccg
     4561 gacataattt taatcaacgt cgatttacca cagccgtttt gcccggcaag gcagtgcact
     4621 tcacccgaca taaagtcgag agaaacatcg cgcagcgctc gcgtggcatg aaacgttttg
     4681 ttgatatgac gaagggaaag gaaggtttcc atagcatccc ctggcaataa gaatcggggc
     4741 tttcgccccg aagaaatgga ttaataaagt gagtcgatat tgtctttgtt gaccagtaat
     4801 accttatgga aacggataat tcgcttatcc atatctacgt cggccttacc gagattctcc
     4861 atttctaatc caggtttgat ctcttcaccc ttcagcaacg tgctggcgac ggccgcgagt
     4921 gcgtaacctg cagaggccgg atcgtaagta atcccttcgg tgatatcacc acttttaatc
     4981 agtgatgccg cctgtgaagg aatcatcatg ccatagactg cgactttatt tttcgcccgt
     5041 ttctctttca ctgcacgtcc cgcgccaatc ggaccgtttg aaccaaagga gacaaccgct
     5101 ttcaagtcag gataggtttt catgaggtcc agtgtggtac gacgtgagtc atccacactc
     5161 tcggcaaccg gcatgcggcg ggtaacttca tgcatatccg ggtaatgctc tttctggtat
     5221 ttcaccagca agtcagccca taagttatgc tgcggcacgg tcaaactacc cacgtaaatc
     5281 acatagccgc ccttgccacc catgcgtttc gccatatgct caacatattc agcggcaaat
     5341 ttttcgttat caatgatttc gatatcccag ttagcacttg gctgaccggg ggattcgttg
     5401 gtcagaacca caattccggc atctcgcgct tttttgaata ccggttccag cacgttggca
     5461 tcgtttggca caattgtaat tgcattaacc ttacgggcga ttaaatcctc aataatttta
     5521 acttgttgcg gagcatcagt acttgaaggc cccacctgtg acgcattaac accaaaggct
     5581 ttacccgcct caaccacacc ttcgcccatg cgattaaacc acggcatacc atcgacttta
     5641 gagatattca ccacgacttt ttccgctgcc tggagcggcg cagaaattag cgcagcgcct
     5701 aataacagcg aagacaccat attgataaca aaacgtttat tcatcatttc agcctcattg
     5761 cagggtagtc aggaagtgtt cgcccaaatt agcgcgctgg agagaataag aattttcgaa
     5821 tatttttatg cagaataaac agccctattt caggcagcta ttaaaaattt ggttataaat
     5881 atggacaaaa cgggcagtaa caataagtgt gacaatcatc acgtaaaaaa tagcaaaata
     5941 aacaaaatta gcgatacttt atgtttgtga gtttatttcg cgtagataaa tagttatcta
     6001 cacaatgtca ataagggtat cttcttcaaa gataacgtcc atttctggtg aatagcttcc
     6061 ttctacctgg aaaaaacgac ggtgttcatt acgccaatat tcaagactga ggtcaccttc
     6121 accttcctta cgggcaagct ctgcggtgac atcagaaaag cgcagtaaat gcaggccaat
     6181 cactctgata acacatacag gttcaccacg gctattttct acaatcttat actcaccaat
     6241 catcggaaat acgttgtctt caatacatcc cgccagcgaa ccacaggaag ctgttttaac
     6301 accagtcgca atcaacgttg cgaggtgatc ggccaacgct gctgaatcgc caaaagtaca
     6361 aaaggaagca tggggatatt tttgtgagaa tttttctttt atggtcatgg caatattttt
     6421 acgattacgg aaaaacaaat cacatcttaa ttactgttga taaccatcag caagagtatc
     6481 attggttttt ctatttttaa tcttttttat ccgaagcagg tcacatttat ggcttgataa
     6541 atacctttgt taaaactcac ttttgtgtat ttctctcaaa taatcccctg gaaattatgc
     6601 cgggagatta taaagttcag gatcattcat taacaaacca ttcccgcccc actccctggg
     6661 gcttaaatct cttactattc accgttgcag gcgtttcaaa tgcgcatcaa cctgccacac
     6721 tcccccacac cagttgccct ttatggaacg ttgcggttct cggcgagata cgcgccaccg
     6781 cctcggcgga acaggaggcg tccaccagca caaagctggc atcatcctgc gctttgggcc
     6841 acacgcgctc gcctttttcg ttgagcggca atacatcgcc agtggctaaa aacagcgagc
     6901 gggagaggtt ctgttcgtta ggacgaatat agagctgcgc gtacagattg gctttttcca
     6961 gcatgtcgcc caggccgtaa ggcgaccagt ggtcgataac gctgtcggtg ccggtcatca
     7021 cttttacgcc tttgtcgtgc aactgtttga gcggcatatg cagcgtgcca atcggcaccg
     7081 tcgaggcgat agaaatttgc tgcgccgcca tccggtgcgc cagttcatct acctgttgct
     7141 cattgagcgt tgccagcgca aaggcgtggc tgatggtcag cttgcccttc agttgcggcg
     7201 ttttctccac cgtttcaacc atataattga tggctgccac gcctgccgga gtggtttcgt
     7261 gcaggtgaat atcaacgcct ttgtcgtagt ccagcgcaat ctggaacatg gtgtcgaggg
     7321 atttttccat cgcgccatca acactggtcg ggtccagccc gccgacgtaa tgcgcccccg
     7381 cctgcatcgc ttcgcgcatt aagggttctg atttcgacag cagcaaaccg tgctgcggga
     7441 aggcgacaat ttcacactcg aagcccgcct gacgtcgcgc cagaaccgcc tgcaaatttt
     7501 gcagattttt caggccggaa accggttcga tattgcagtg gctgcgggca atggtagtgc
     7561 ctttcgactg caataaatca atcagttttt ccgccctttc ctgagtgtac gattgcaact
     7621 ccggcagcat tttctgctcg agtttgatca tgtcctggat ggtggtgcct gccggacgat
     7681 tgagcgagcg ccacggcccg ccgtagaagg ttttgtcgag atgaatatgc atgtcgcggg
     7741 tggtgggcag catcagctta ccgccagcgt catagtgcgg cagcgtggcg tccggatgct
     7801 gattgttctc gcgcagtgca acaattttgc cgttctgaat ttccacagtc tggcgcgccg
     7861 tgcgggtctg caccgccacg ccattttcgt agtcaaaacc ggtttccagc aacacgttgt
     7921 cgagatagta gtgcgggtca gtgattttca tggtgttatt cgcgtcgcag tgggttgtgc
     7981 cagagactgc cgcatgggcg agcggcacag aggtaccaaa cagcgcggcg gcggtgacca
     8041 tcttaccgct ctggctcaga aactcacggc ggctattgct ttctttcatc ttacgtcctt
     8101 acgttttaat aatatgggtg cccgtttcac cgcgcagcgc cgcaggcagg cattccggcg
     8161 tggtgataat cacttctttg ccgccctgtt ccaggaacgt caggctggcg atgatttttg
     8221 gcaacatgct gccaggcgga aaatgccctt cctgcatata gcgggtcatg gtggcaatat
     8281 ccacccgatc gagcgcctgc tgctgcggtt tgccaaaatg aatacacact ttttccacgc
     8341 ccgtggtgat caccagaatg tcggcgtgaa tttcgcgagc cagtagcgcg gtagagagat
     8401 ctttgtcgat aaccgcgtcc acgctttggt aatctcccgc ttcggtgcgc accacaggaa
     8461 ttccgccgcc gcccgcgcca atcaccacaa agccttgttg gatcagcgcc ttaatggccg
     8521 gtgcttcgac aatacgtttc ggttccggcg aggcgactac gcggcgatag ccccgtccgc
     8581 catcttcaac aaaacgccag tcagggtttg ccttttgtaa ttcgtcacgc tggctgtcac
     8641 taaagaatgc gccgatgggc ttggtgggat gggcaaatcc cggatcgttt ttatccactt
     8701 ccacctgagt caccacggtg acggcttttt tctcgccgtg gcgggccagc cggttgttca
     8761 gcgcctgctg gatcagatag ccaatgccgc cctgcgtatc cgccacacag tttgccagcg
     8821 gcgttaaggg cagcccttcg cgctcgtggg caatctccgc acggcgtaaa tccagcccga
     8881 cctgcggccc gttgccgtgg gtcagcacaa tgtcgtaatc ggaagccagc atttccagca
     8941 ccgtatcggc gacggctttc accgcctccg cctgatgctc aatcgactgg ctggcgttat
     9001 ctttgataat gctgttgcca ccaatggcaa cgaccacaag ttctttcatg ttgttatgtc
     9061 cgctacagag ggtgatgggg ttatgagagt gaggttgccc ctctccccag aagggagaag
     9121 ggatcgtaag gtgcagttgg agggtcagac gccgtaacgt tcgcaccagc caagaatggc
     9181 tttctcgaag caggcgaatg gtggatgcac aatgcccgcg ccaatcatgc caacgcccgc
     9241 atctttatgg gcaatggcgg tattgatcac cggcaaaatg ccgctgctgc ccacgcgggt
     9301 gatgtcgatt gccgacggca cgcccataaa accgagcagc ggaatggtga cgttggggtt
     9361 ttcaccgaga gtgatttcac gcatctggcg ggagaaatcg atagcctctt ccaccgtacc
     9421 gcccaccagc gcgacgatag caggcgcggt cgccatggca aatccgccaa tgccgtaggt
     9481 ttcagtgatg gcgctgtcgc caatatccaa tccagaatct tccggcttat aaccggcaaa
     9541 catcgggccg atcacctgct gcgccggacc ggtaaaccac tgccccggta atccgctgac
     9601 ccgcaggccg aactcgacgc cgttacgcgc catcgtggtc accacggtgc tgtattcgat
     9661 gccgtgcgcc gcatccatcg ccgctttaca catcgccatc cacgtcgggc cggagaagta
     9721 gtcgctgctg gcgacaaact caaacacttc gcgctgttgc tcgacggagt agcccgcctg
     9781 aataatcccc ggcgtcagcg cctgaatcag cagcgtcgtc ccggcgttat tgcggttatg
     9841 gcactcatcg cccatatgca gcgcctgcgc cagcattaag cgcagatcga tttcgccgat
     9901 aattttcatc gcgtcgcgca gcatgggtcc ctgcacatcg cgcatccagt tgagtcggtc
     9961 gatcacgctc tggtcgttag cgcccatacg caaaatcttt gccatctgct cgctcatatt
    10021 ggtgtaagcg atgttgccgt aggttttgtt tttcacgatg tgcataaaca tcgaggccga
    10081 ggtaacaccc gccatcgatc ccacgcagtc atgttcgtgg cacggcgaga aggtgatctc
    10141 cccggaggcc gccagttcag ccgcctcatc gagatctttc gccagccctt cgaacaccag
    10201 cgctccggtg accgcgcctt tcatcgcgcc acacattttt tcccaggtga tcggcggtcc
    10261 ggcgtgaaga atggttttcg gcgtcatacc cggcaccacg ttaatcgcct gatcaaaacc
    10321 aatcagcacc ggatgcgact ggataatgcg ctccaaagcc tgctggttag cggcggcgat
    10381 tttgtctgcc agcggggaat cggcaatgtt gtccagcgcc tgcaccacct gcatattgcc
    10441 ctgccccggc ggcgtccagt cgagttgggt cacttctaca tgctgctttt tcagatcatc
    10501 gctaaacatg gcgatgccga cgttaataac gttcaatggt tggctaaaca gtgactggct
    10561 catcaggctt cctccccttt gcagataaat tcacgcgcca gcaatccggt attggtgcta
    10621 ctgctggcca gaattacccc agcatcaagc agcatctggc tttgttgttc taatgatggc
    10681 gtatcaagat cggtacccag cacataggcg agaatgatca actcacgtcc ttcggcagcg
    10741 gcgatcgctt tcgcctcttt gatcgcctca atggtggaac cgaccggatc ttcatgcgat
    10801 ccaaatccga gcacaaaatc catcacgatc accgccactt ctggatcgcg cgcttcttcg
    10861 atcaaccggc tgatgcggtt ggtggggtca atcattggat gcggcttgcc gttggtgaag
    10921 tcgtcatcgc caaagtcgag gaaggtgtgt ttgatgctgc ggttaatatc ttgcaggcgg
    10981 aattcaggat cgggctggat gttgctgtag acatcgccgt gtttttccat caccgcgaac
    11041 atcgtttcgt cgcacagcgt gccgccgcag aacaggccac gaatgtattt ctgttgcggt
    11101 tgcaggcgag cgcgcacgtc agcaatcaac ggctggttca gcgtatgcag ctcgagatgt
    11161 tcctgtttca cgccggagag cattaccgct ttcagcgccg cttctttgct gccgcgagca
    11221 aactgtagcc cctgctcatc caccggcgtt tcaacgcgac cgaggaagca gaccaccacc
    11281 ggcttgcggc aggcacgtgc gcgttccagc actttgcggg ccaccgcagg cgcaggcggt
    11341 ttggagataa gcacaataat ttctgtttgc ggatcgtttt ccagcatccc gatggcatcg
    11401 agcatcatca gaccgccgat tttctcgctc aggtcgcgcc cgccggtgcc aatcagttgc
    11461 gaaacgccgc cgcaaaattc atggatacgg acgctcagtt cctggctgcc ggtgccagat
    11521 gcgccaacaa tgccgatgtt gccgcgacgc acggcgttac caaagcagag cgccgcggcg
    11581 ttgataatcg ccgtaccgca gtctggcccc atcatcagca gccctttttc gtgggccaac
    11641 tgtttgagcg ccagttcatc ttcaactgag acgttatcgg aaaacagcat cacgttaaga
    11701 tcgttttgca gcgcctgacg cgcttcgcga gcggcaaaca gaccgttgac cgaaatcacc
    11761 gccaggttac tttccgggat atgctttttg gcgctggcaa tagtggcata gcgtgcttca
    11821 tgtgaaccag tttgagcttt ggtgttgaac agttcttcaa tctccaccag caactgctcg
    11881 ttgtctgcgc ccgctttgcc gttgatgaca atcatcaggt cgccgttttt cgcctgctcc
    11941 agttccggcg tcagcagccc taaattcttc agcacgcctt tgttcatttc ggtcgccatc
    12001 gccacaaatg cctgctcgac gccgtcgagt ttatttgcac gcgtggagat agacatcagc
    12061 gagacagaat caaaataggt attcggttta atgactattt taactgacat aattccctcc
    12121 gtaggtttgg ctcagcgcac aaccatcggc catgccatac agcatgtcgc agccggaact
    12181 ggagccaata tgtttaattt tttctattgc ctgagttgcg ttcccaggga tgtcataaat
    12241 aatgttatga ataaaacgat gaatattttc ccggcagcgt tgttgtaatg cggcttccag
    12301 cgttatggca cttaataacg tggtattatt tctgccgcgt tgcagaccga gataaaattc
    12361 ctctttgtat ttttccgccg gatgtccgga aataaataaa ataagcgcca gacctgttaa
    12421 ataatcgtcg gctgatggcg taaggccaat ccctaagccc atcatactgg cgactgctga
    12481 ggcggtattt tgtttatcgt ttacggcctg aataactgcc cgtcgtttaa tatgtaattc
    12541 tttatttaac gcctgataaa acggattatc gccgcaataa ttaaacaggg tttcattttc
    12601 ccccaggtgc tgatgaataa tgtcatgcca ctgttgccag gcgataaggt gaaaattcac
    12661 agcggccatt tgcaaggttg gggacagcca acgcaggcaa tgactcatct ctatatgaag
    12721 atgttgacca accgtaatgc cttgatcgtc aaactgaacc ttatcaccat gccggaacag
    12781 accatcaaag tgagtgagtg ccaaccgaca actgtttggc gcattgtcgt actcttcaca
    12841 aagcagcgtc agcaactgct ggcgggccgg aagtgcgata tttaccgctc tggcaaagac
    12901 ctgttcaacc tgaccttgcc cccgctgcgc cagaaaatca gcatccgcag ttagcgccca
    12961 cacacaccgt ctttctggtg tgttgatagc aagcctcccg gtttatgcac ctgcggcaat
    13021 cagtaactgc gcaatctctt caaagcctcg ttcccgcgcc agttccagcg gcgttttgcc
    13081 atatttatcc gtcatgtgcg ggctggcacc gtgttccagc aataactgga caatcgcctg
    13141 ctgtttaatc ccgccatcat taagcacaat tgcttccagt agcggcgtcc agccaacatg
    13201 gttggtctgg ttaacgttaa tctccgtatg cgccaaaagc tcttttacaa tacttaaatg
    13261 gcctttttca caggcaggcg tcaggccgac accgccaaaa cgggtgacgc aattaagatc
    13321 aggtttagcc ggtaaaataa tccgtaacag ggttaaatcg ccggtcaggc agctaattaa
    13381 aaaaggattt gaacatattt gatcttgctt attaatatcc gctccggcat caattaatgc
    13441 ctgaacgcaa gcatattgct gatataaact tgccagcgta attgccgttt tcccctgacg
    13501 atcgcaggta ttaatatcga tacccagcgc aaggcaggtt tttactttat cgatatcacc
    13561 ctgttgcgca gccaataaat agtcggcagg tagatttttt atagtcattc acattcccct
    13621 gatttttgtg gctgttttcg atgaattgag aatagcaacg ataatctgat aaaagaaccc
    13681 gcttaaattt aattcgccgt atttcgcctt tttgttgaat gatgttcaac aatcttttcg
    13741 cagtgtgcgg tagtgcaata tttgtttctt tgacccgata ccctttcact caatcaggtt
    13801 ttatttctct atagtctgca catgtaagag tagctttcgt ccatgtctaa ccagagggga
    13861 acaagatgaa cggactcacg gcaacgggtg tcaccgtcgg tatttgtgca gggctttggc
    13921 agttagtgtc ttcacatgta gggctctctc agggatggga attattagga accattggct
    13981 tcgtcgcctt ttgcagtttt tatgccgcag gcggcggtaa atcggggttt atcagaagtc
    14041 tggcggttaa ttattccggg atggtgtggg cgtttttcgc cgcgctggct gccggctggc
    14101 tggcctcggt gagtggattg tccacgttct gggcgagcgt gattatgact gtacccttct
    14161 cggcggtagt ggtctggcaa ggacgtttcg gtttattgtc ttttattccc ggaggctttc
    14221 tcggcatgac gctatttttt gccagcggga tgaactggac ggtgacatta ctgggttttc
    14281 tggcgggtaa ctgcgtgggg ataatttcag aatatggcgg acaaaaatta agcgaagcga
    14341 cgactaaacg cgacgggtat taaaagttat tcaaaaatga ttatctatct ttaaccgata
    14401 cctctacagt catggctgga gaaattcata gtagataatg gcgggaatgg tttaatgaac
    14461 tcaattttta ccgaagagaa tctgttggca tttactaccg ctgcgcgttt tggcagcttc
    14521 agtaaagccg cagaagagtt gggtttaacc acttccgcca ttagctacac catcaagcga
    14581 atggaaacag ggctggatgt ggtgctgttc actcgcagta cccgaagcat tgagttaacg
    14641 gagtccgggc gttatttttt ccgcaaagct accgacctgc tgaatgattt tcacgccatc
    14701 aaacgcagca tcgatactat ttcgcagggt atagaagcac gggtgcgcat ttgtattaac
    14761 caactgcttt atacgcccaa acacaccgca cgcttattac aggtactgaa aaagcagttt
    14821 ccaacctgcc agattaccgt gacgaccgaa gtgtataacg gcgtctggga tgcgattatt
    14881 aataatcagg cgaatattgc tatcggcgcg cccgacacac tgctggacgg cggcggcatt
    14941 gattacacgg agattggcgc aattcgctgg gcttttgcta tcgccccgga tcacccatta
    15001 gcctttgtgc cggagccgat cgccgaaagc caactgcgtc tctatcctaa tatcatggtg
    15061 gaggacaccg cgcatacgat taataaaaaa gtgggctggt tgctgcatgg gcaggagtcg
    15121 attctggtgc cagatttcaa caccaaatgt cagtgccaga ttttaggtga agggattggt
    15181 tttttgccgg attacatggt ccgtgaggcg atggcgcaat ccctgctggt tacccgacaa
    15241 atccataatc cgcgccagga ttcacgcatg ttactcgcaa ctcagcattc tgctacgggt
    15301 caggtgacgc agtggattaa aaaacaattt tcgccaaatg gcatattaac ggggatctat
    15361 caggatttac tgcatcggga gaattaatcg ctgccaacct cccgatgcgc tgtgaatagc
    15421 ggtttacatc aaccacctgc tttcattacc cattcagaga taaatttatt acccggtact
    15481 ggcggagagt acaaatagcc ctgcaaaaag tgaacgcctt tgccgatcat taaatccgcc
    15541 tgctcctggg tttctacccc ttccgccacg atactcagac caggcttacg cgccagttcg
    15601 acaatattgt ccacaatatg accggatatt tcgtccacgc tcgccatttg cacaaatgac
    15661 ttatcgatct taataaaatc gaccgggaac gcctgcaagt aacgataggt cgcataaccc
    15721 gtaccaaagt catccagcgc aaaggtaatg ttgtgctgat gaaggctgtc aaatatcgct
    15781 ctggcttctg gcgttaccgg aatagggtta cgttccgtta gctcgagaac cagtttgatt
    15841 ttatcgttac ctaatttctt aaccaggttc aggcattctt tttcaaatcc cgcagccaaa
    15901 aaacaacccg ccgagacgtt gatgccaata tggaaattgt ccggcagcaa atgttttacc
    15961 ggcatcagaa tatccgcagt ctgtttcatc aactggcgag tcattatgac gataagacca
    16021 gatgactccg ccagaggaat aaactgatcc ggtgggataa ttcccgtttg tggatgttcc
    16081 cagcggacaa gcacctcaca gcccgtcagt accccagtct gcgcgcagaa aaccggttgg
    16141 atccacggtt tgaattcgtg gttttccaac gccagactga tggcttcagg cgtgacgata
    16201 tggtgatagt gattatcatt aatatatttg tgactattat tacccgtggc ggatatgatc
    16261 tccggattgt actgaaagaa aatatcccgc cagaaagtaa tatcgctctg aatccccagt
    16321 ttctcaaaga gctgtttctt ttgatgtgaa atggtctttg cactgcgatt tctgtactgt
    16381 gatatttctg ttacagacat tccctgcatc agcagcttga gtagcagtcg ttctttctcg
    16441 gatacgctac caggcaatga caaatgaacc gttgtaccga actcttgcag aaaaacacga
    16501 aaatcacatg aattcatgaa cacaccttta tcttttatcg ctgttcagta tgagtgatta
    16561 cctgtggatt cctgagcaca cataaggggt aaccataaac atcagcgatg ccatacgcat
    16621 aacatcccac atcactatca aaaagattat ctttacctct gatgctcgct caaaaacagt
    16681 tacgctaaat accttaaaaa ggtaatcaca agtcaaactc aggagataat cacgcaagaa
    16741 tgcaatatta aaattcagat actcattttt ggtaataaag aagtagttgg actatttcaa
    16801 tatttataat ttcccactac gtattgacgc ccattcatga gtaaaggcgc attatatgaa
    16861 tttaacaatc cagatcaact gaaaatacct caacctcata aacacatagc gtcaacattc
    16921 aatgacataa tgagtaaaga tgttggttat gcatacacgt cattactcta tgcctatccc
    16981 ttaacaacct gctttttaag gctgaatcca ttcagcaata gatagccaac attataacaa
    17041 tgacaataaa aacaatattt aaaacaatca ataaaatatc agacacagac aattaaacct
    17101 taaacaacaa acagttacat tgattttagt tgatttgcgc tacgcgtttc ccatgaataa
    17161 agcgtcattt caaattagca ccaatattta cattaaataa gactaagctt attgaacatt
    17221 cacaacagtc caagcgtagg ttttgggctt caatagttct tttctttttt attatctgtt
    17281 gcctggcaat catcaatcga tactaagggc ttaactgtac acagatataa cgtatcaaga
    17341 ggtaagagaa accgcgccat atgcagatat ggcgcggttt tatgcaataa caatcacgcg
    17401 tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatggataaa gttaaggtgc
    17461 cgctcgtact gatcgagaat atcggtgatc acctgctctt tgctgtagtc catcaggtcg
    17521 ttgccctggc tgccttctaa caggaaggtt tccagccggt agtaggtcga tttcccgcta
    17581 cgtgcgcggt aggtaaagcc cggcaccgag tattgctgcg gccaaatctg atagacaaag
    17641 ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg tccctcttcc
    17701 ggcggcaggt tttttagctc cacgtacgcg ccgcgcaacc gcagctcctg cgccacttct
    17761 tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt gcctggataa
    17821 ttcatcaggc gcgagagacg ttttttccag ctcaggcgat cctgaagccc cagcggtcgc
    17881 ggcgcggtat cacggttggc actttcacgg cggtaatctt ctaccttcag agatttatac
    17941 aaccccgcca tcacgaagaa aatcacaaag ctgaacggca gacccataat caccgtggtg
    18001 ttttgcagcg cggatatccc gttggtcatc agcatgccga gcgtcagcag gccaatcgcc
    18061 accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag
    18121 gtgaaattcc ccagcaccag cgccccggag tccgccgagg tcacataaaa cagcaggcca
    18181 gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag caggctgtag
    18241 aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt
    18301 tcatacagcg cgctattgcc gaacaccgag agccataaca gcgtgaaggt aaacggaata
    18361 atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg cgccaggaac
    18421 aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt ccagttattc
    18481 atccactcaa ccggacggtc gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc
    18541 acatagtcgc caacgttcag caccagcgca ttaagcagga acgaggtgtc gcccataaac
    18601 agcacgaaca ggatcaaccc cagcgccagc gcaacgttga gttccgataa cacgcgaatg
    18661 cccttatcga caccggaggt gacagagatt gttgcgatga tcaccgacaa ggcgatcagt
    18721 gccgctttcg ccgccatcga atcaggaata tcaaacagta cgctcaagcc atagttaagc
    18781 tgcaccacac cgataccgag cgtagtggca ataccgaaga tggtgccgat taccgctgca
    18841 atatccactg aatgaccaat cggcccgtta atccgtttac cgaagatcgg gtacagcgcc
    18901 gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc catgcccatc
    18961 agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac catcgcctga
    19021 cgcgcagcct caatcgtctg acccgcgcct tccggcggct gcatatactg cgttaccggt
    19081 tcggctacgg agaagaacat caggtcgata ccgatcccgg cagcaaacag catcgccgcc
    19141 caactcagca gactgaattc cggtttggat tgttctggcc cgagcttcac cgaaccaaaa
    19201 cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag cagatagtac
    19261 caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc cgagaagtcg
    19321 cgaaacagga tcgttgtcag ggaaaacaac aaaatcagtc cggcggaggt gtaaaacacc
    19381 accggattga ttttgtcctt ttccctgctg tgtgaaaggt ctgtcatcca gtatccccac
    19441 tgttattgtt accatttaaa atcaaattcg taacaattaa gacacatttt atattgaacg
    19501 tccaatcaat aaccgcttta atagataaac aacgctgatg aatggagtgg cgaaaatgcc
    19561 caaattgggg atgcagtcga tccggcgcag acaactgatc gacgccacgc tggaagcaat
    19621 aaatgaagtg ggcatgcacg atgcaacgat cgcgcagatc gcccgccgtg caggcgtttc
    19681 tacggggatc atcagccact atttcaggga caaaaatggt ctgctggaag caaccatgcg
    19741 cgatatcacc agtcagctgc gtgacgcggt tttgaatcga ttacatgcac ttccgcaggg
    19801 cagtgcagag cagcgattac aggcgattgt tgacgggaat ttcgatgaaa cgcaggtcag
    19861 taatgctgcg atgaaagcct ggttggcgtt ttgggccagc agtatgcatc agccaatgct
    19921 ttaccgtttg cagcaggtga gcagccgccg cttgttgtcg aatctggtga gcgaatttca
    19981 ccgcgagtta ccgcgccaac aggcacagga agcgggctac ggccttgccg cgctgattga
    20041 cggattatgg ctgcgcgcgg cactgagcgg caaaccgctg gataaacctc tcgcacactc
    20101 gctgacccgt cactttatta ctcagcattt acccaccgat taaccgagga gacgtgatgt
    20161 cccgaatggc agaacagcag ctttatatac atggtggtta tacctccgcc accagcggtc
    20221 gcaccttcga gaccattaac ccggccaacg gtaacgtgct ggcgaccgtg caggccgccg
    20281 ggcgtgagga tgtcgatcgc gccgtgaaga gtgcccaaca ggggcaaaaa atctgggcgg
    20341 cgatgaccgc tatggaacgc tcgcgtattc tgcgtcgggc cgttgatatt ctgcgtgaac
    20401 gcaataacga actcgcaaaa ctggaaaccc tcgacaccgg aaaagcgtat tcggaaacct
    20461 caaccgtcga tatcgttacc ggcgcggacg tgctcgagta ctacgccggg ctgatcccgt
    20521 cgctggaagg cagccagatc ccattgcgtg agacgtcatt tgtctatacc cgccgcgaac
    20581 cgctgggcgt agtggcaggg attggcgcat ggaactaccc gatccagatt gccctgtgga
    20641 aatccgcccc ggcgctggcg gcaggcaatg caatgatctt caaaccgagc gaagtcaccc
    20701 cgcttaccgc gttaaagctg gctgaaattt acagcgaagc gggcctgccg gacggtgtat
    20761 ttaacgtgtt gccgggcgtg ggcgcggaga ccgggcaata tctgaccgat catccgggca
    20821 ttgctaaagt gtcatttacc ggcggtgtcg ccagcggcaa aaaagtgatg gctaactcgg
    20881 cagcttcctc cctgaaagaa gtgaccatgg agctgggcgg taaatcgccg ctgatcgttt
    20941 tcgacgatgc agatctcgat ctcgccgctg atatcgccat gatggcgaac ttcttcagct
    21001 ccggtcaggt gtgtaccaac ggcacccgcg tcttcgttcc ggcgaaatgc aaagccgcat
    21061 ttgaacaaaa gattctggcg cgcgttgagc gcattcgcgc gggcgacgtt ttcgatccgc
    21121 aaaataactt tggcccgctg gtcagcttcc cgcatcgcga taacgtgctg cgttacatcg
    21181 ccaaaggcaa agaggaaggc gcgcgcgtac tgtgcggcgg cgatgtactg aaaggcgatg
    21241 gcttcgataa cggcgcatgg gttgcaccga ccgtgttcac cgattgccgc gacgaaatga
    21301 ccatcgtgcg cgaagagatc ttcggtccgg tgatgtcgct tctcacttat gaatctgagg
    21361 acgaagtgat tcgccgcgcc aatgataccg actacggcct ggcggcgggc atcgtgacgg
    21421 cggacctgaa ccgcgcgcat cgcgtcattc atcagctgga agcgggtatt tgctggatca
    21481 acacctgggg cgaatcccct gctgagatgc ccgttggcgg ctacaaacac tccggcattg
    21541 gtcgcgagaa cggcgtgatg acgctccaga gttacaccca ggtgaagtcc atccaggttg
    21601 agatggctaa attccagtcc atattctaac caggaggttt atttgcaatt tgactacatc
    21661 attattggtg ccggctcagc cggcaacgtc ctcgctaccc gtctgactga agatccgaac
    21721 ataaccgtgc tgctgcttga agcaggcggc ccggactatc gctttgactt ccgcacccag
    21781 atgcccgccg cgctggcatt cccgttacag ggtaaacgct acaactgggc ctatgaaacg
    21841 gaacctgaac cgtttatgaa taaccgtcgc atggagtgcg gacgcggtaa ggggcttggc
    21901 gggtcatcac tgatcaacgg catgtgttac atccgtggta acgcgctgga tctcgataac
    21961 tgggcgcaag aacccggtct ggagaactgg agttatctcg actgcctgcc ctactaccgc
    22021 aaggccgaga ctcgcgatgt aggcgagaac gactaccacg gtggcgatgg cccggtgagc
    22081 gtcactacct ccaaacccgg cgtcaatccg ctgtttgaag cgatgattga agcgggcgtg
    22141 caggcaggct acccgcgcac ggacgatctc aacggctatc agcaggaagg ttttggcccg
    22201 atggatcgca ccgtcacgcc gcatggccgt cgcgccagca ccgcgcgtgg ctatctcgat
    22261 caggccaaat cgcgccctaa cctgaccatt cgtactcacg ccatgaccga tcacatcatt
    22321 tttgacggca aacgcgcggt gggcgtcgag tggctggaag gcgacagcac cattccaacc
    22381 cgcgcgacag cgaataaaga ggtgctgtta tgtgcaggtg cgatcgcctc tccgcagatc
    22441 ctgcaacgtt ccggcgtggg tagcgctgaa ctgctggcgg agtttgatat tccgctggtg
    22501 catgatttac ccggcgtcgg cgaaaatctt caggatcacc tggagatgta cctgcaatat
    22561 gagtgcaaag aaccggtttc cctctaccct gccctgcagt ggtggaatca gccgaaaatc
    22621 ggtgcggagt ggctgtttgg cggcaccggc gttggtgcca gcaaccactt tgaagcaggc
    22681 ggatttattc gcagccgtga ggaatttgcg tggccgaata ttcagtacca tttcctgcca
    22741 gtggcgatta actataacgg ctcgaacgca gtgaaagagc acggcttcca gtgccacgtc
    22801 ggatcgatgc gctcgccaag ccgtgggcat gtgcggatta aatcccgcga cccgcaccag
    22861 catccagcga ttctgtttaa ctacatgtcg cacgagcagg actggcagga gttccgcgac
    22921 gcaattcgca tcacccgcga gatcatgcat caaccggcgc tggatcagta tcgtggccgc
    22981 gaaatcagcc ccggtgtcga atgccagacg gatgagcagc tcgatgagtt cgtgcgtaac
    23041 cacgccgaaa ccgccttcca cccgtgcggt acctgcaaaa tgggttatga cgagatggca
    23101 gtggttgacg gcgaaggccg cgtacacggg ctggaaggtc tgcgcgtggt ggatgcgtca
    23161 attatgccgc aaattatcac cggcaatttg aacgccacga cgattatgat tggcgagaaa
    23221 atagcggata tgattcgcgg gcaagaagcg ttgccgagga gcacggcaag atattttgtg
    23281 gcaaatggga tgccggtgag agtgaaaaaa tgagtcgtta tgtgaactga ctcaggaacc
    23341 gattagattg agagttaccg ttctactatg aggaccgaat ccttatataa aacactgagg
    23401 taactctcat gcttcatatc cagtattcat gatgctggtt ttttgctgat atttaacccg
    23461 aacactcctg atacagacta ttaattcatt tatctgcgac agatttcatt ttctggatat
    23521 attatgagtg aacaaatcaa acaggatatc gatctgattg agattttatt ttatctgaag
    23581 aaaaagatgc gtgttatcct ttttattata gctatctgta tggcaatggt gcttttattt
    23641 ctgtatatca ataaagataa tataaaagtg agttacagcc taaaaataaa ccagacaaca
    23701 ccaggcatac ttgttagctg tgatagcaat aataactttg cctgtcagac cacaatgact
    23761 gaagatgtta ttcagcgaat tactacattt tttcacacca gcccagatgt caagaacaga
    23821 gaaataaagc tggaatggtc aggagataag agagatttac caactgctga agcggaaata
    23881 tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag aatatcataa tggcaggcaa
    23941 gttctcgatg agatacaaac gccttcagca attaacagtg agctttatac aaaaatgata
    24001 tacctgacca ggaactggtc attgtatccg aacggtgatg gctgtgtaac tataagctca
    24061 ccagaaataa aaaataaata ccctgctgcc atttgcctgg ctctgggatt ttttctaagc
    24121 attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg tagatgaata ccaacaaaac
    24181 tctgggcagt aataaacagc attctgacag agagtgcctg tataaagctg aagtgacaat
    24241 gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg taaatatcct
    24301 ggcattacta atgccaggat atttacagaa gagcagcata accatgacgg aacggggtta
    24361 agtcaggttg tccttaacgc tattcacaac ctcgtatgct gccttcaggt ttatgtccag
    24421 acttcatatc tctctcagca atcctcaata atcagataca ccgctttcac cgggccatga
    24481 accccgacga ctttgataag ctcaatatcc gccgttgaac tagggccgct aatgatgtta
    24541 atgcaggacg gcattcgttc accggcctgc gctttctgat gcaatttttc tgcgagttgc
    24601 gctacacgcg gcaggattgt gcttttacgc aggataaaaa gagaagattc cgggagcagg
    24661 ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa
    24721 ccatattcag catacacgac acccacttta gcctgctctg cctgtgagat attctcggca
    24781 cctttcgccg gatcccaaac aacgacattg cattcctgct gcaaacgttc gctaatcccc
    24841 aattcctcca gcctcgtgtc accgctaatc acgaccgact gatctcccag ctctttacac
    24901 agacgtattg cagcttctgc cgccttcgcc tcgctggtca actcacagcg cgtcaacata
    24961 acatcgctgg caaactgaat aaacgcgtca cagcgctgct gttggttaag tgcggtaagc
    25021 cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag tcgcagcggg
    25081 cgacccagtg cctgagcaac gttattcaaa aattcgctcc gattatccat tctttttctc
    25141 ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt caggaagatc
    25201 gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc cgccattgat
    25261 aaaccaactt gccgcatgag cgccggccat catcccgact ttccacaatc ctggatgact
    25321 attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga tccctttttc
    25381 agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca ccggacacac
    25441 gttgtcacaa gcagtgcata aagagcaggc gtagggtaaa tctttaaaat ctttatagcc
    25501 gccaagtagc ggagaaatca ccgcaccaat tggccctgga taaatagagc catatccatg
    25561 accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcagc gaatacagcg
    25621 cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga caataaccag
    25681 atgaaactct tcaggaccat caacgtgccc agcttcgcgc ggtcctgtca gccaggtgtt
    25741 gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca atacatctac
    25801 ctcggcaaac gtgggagcaa tacgctccat tcccatcact gcaatatgcg ttttaggcag
    25861 cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac cggtctctgc
    25921 caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt cgcggatttt
    25981 ttgccgaatg aataaggtca ttgcttcagg cgtttccggc ccctcatagc ccagacgttc
    26041 atgtagcact cgacggatct gatggcgatc tttatgaatt gccgggacca caacatgaga
    26101 gggcggatct tgatccagct ggagaatgta ttcacccaga tcggtttcaa tcacctgaat
    26161 cccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg atttagattt
    26221 caccaccttc cggacatttt tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc
    26281 tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc actttttctg agagctggta
    26341 cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc gatcgcgcca
    26401 ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccga tacgctgctg
    26461 cgcgtttgcc accgctttgc gcatgatagg atcttcaatt tgctgacgga tgcgtgtctt
    26521 aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc atcaacactt
    26581 cagcaatatg catcactttg actttctgcc cttcccgttg taatcgtcca ctgatgttca
    26641 gcaggcaact cacgtcagca ccaattaaat actctgggcg gacttccatc aggtgcgcaa
    26701 ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac gtgccgccaa
    26761 atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc acatttttca
    26821 gcagcataag tggctcgtcc ttcaccccca gcttacgggc caggctacaa gatgggtgat
    26881 acaccgctct cccttgcaaa ctggcaccta catcgactac ccctaatgta ttaacaataa
    26941 aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt
    27001 catccgccag ataagttggg taacttttta cggcataggt acaggagcca gccggtgaaa
    27061 taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct ggaatcgctt
    27121 ctttgatata accactattg atcgcaggct gaccgcagca tccctgtttc tccgggaaat
    27181 ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt cttgatttca
    27241 gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact ccattacttc
    27301 atgcccattt atgcccgatg gttcccccct tccgggggat caatcatcaa taaatatggc
    27361 gttctttttt aattggctag caacatacca cccctcattt gtatgacaaa tgataatctt
    27421 tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct cacattttat
    27481 agggaaatcg acgatcattt ttggcataag aaatccattt aaaaaggcta catcccttat
    27541 acctattcag tgctacttat tcaggggtta ttgagatata cactgatggt gtacaaacaa
    27601 aatcaccaac aataattacg tatcaagtaa taatcggcat tttatttaat tgaagcaatt
    27661 tagtttgcat ttttatttaa aaactgagct agagtccatc agaaaaaaat atataaagcc
    27721 caaaccaccg ttggaaatag aaatagttga aaggctcaca actcccggaa tgcggattat
    27781 ctttgacctg atttctggcg gttttattca gggtgcaagc tgtctgaccc tttcccgata
    27841 ttctcccgga gtacaaccaa actcgcggac aaacgccttg tgaaaagatg attcactggc
    27901 atagcctact gactcagcga tcaccacaac agggagcatt tcccgggaaa acatctgggc
    27961 cgctatttgt agacgcaact ttgttaatac agctagcggc gtggttccgg aaacatcacg
    28021 gaaaagctgg gcaaaacttg cccgggacat gtgggcgatg ctggccagcg attcgacggt
    28081 ccaggcgtgt cctggcattt ccagcatttg ctgtattacc gcaccaagac gtggatgcag
    28141 aagcaaactg agaatgtttt tctctgtatt aacctgtgca atccactcac gcaccgcgag
    28201 ggtaaagaat gtagcgcaga tctggctaca cagagcatcc actcccggca ttgccgatct
    28261 ggattcctgt tgtaaaaacg ggatcgcctc cctcagccag ttatattcca cgctatggtt
    28321 cactggtgct acaaacagcg tttccggcaa agacgttaaa aaataacgcg ccgaatgttg
    28381 cagccgaaga gtgccacaga caatacaggt cgattcatta tcgacatgac tcaggcgatg
    28441 agctgaattt tgtggtagca ggaccacatt tcccgggcgt aatgtaaaaa tctctcccgt
    28501 cggcatttcc agcttcgccg ctccttgcgt taacgcatgc caacgaataa ccgacaattc
    28561 cccggcacca tgtggaagct gccagtcact tcctaacacg caattcttat cgatcgttcc
    28621 ttgtggagcg ttaagcatca acaaacggct aagagcatcc atatcagact cctgagtagt
    28681 tttttaatta caaacaccaa ctatcttagt caataattaa tctaatacca tcaaatagtt
    28741 aatcatgatt tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca
    28801 aaatatcaag cgttgtgaag aaatgttatt tgctcttttg cgtctatagt catgatgtca
    28861 aatgaacgcg tttcgacagg aaatcatcat gaataaatat caggcagtta ttattggttt
    28921 tggcaaggct ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gtgtggctct
    28981 catcgaacaa tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gtatcccaac
    29041 caaaacattg gttcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa
    29101 aaatgaagtg gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa
    29161 tatcgacgtg atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg
    29221 gcctgaggga aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac
    29281 cgtggttccg ccaattcctg gaattaccac cacgccagga gtatatgaca gcaccggatt
    29341 acttaatcta aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt
    29401 tgagttcgcc tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc
    29461 gctgtttttg cctcgggaag atcgggatat tgctgataat atcgcgacga ttttacgcga
    29521 tcagggcgtc gatataatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca
    29581 agtgcaagtg catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg
    29641 tcgtcaaccg gctaccgctt cgttacatcc agaaaatgcc agtatcgcag taaacgagcg
    29701 cggggcaatt gtcgttgaca agcgattaca taccaccgca gacaatattt gggcgatggg
    29761 agatgttacc ggcgggctgc aatttactta tatttcactg gatgattacc gcattgtacg
    29821 tgatgagtta ctgggtgaag gcaaacgtag tactgatgat cggaaaaatg tgccttattc
    29881 cgtatttatg acaccgcccc tgtccagggt tggtatgaca gaagaacaag ccagagagag
    29941 tggtgctgat attcaggtgg tgacattgcc tgtagctgca attccgcgtg ccagagtgat
    30001 gaatgatact cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg
    30061 ggcatcactg ctgtgtgttg actcccacga gatgattaat atagtgaaaa tggtgatgga
    30121 tgccgggctg ccttatagca tattacgcga tcagatattt actcatccgt c
//
LOCUS       DAOTRK010000053        30041 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.053, whole genome shotgun sequence.
ACCESSION   DAOTRK010000053 DAOTRK010000000
VERSION     DAOTRK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.053"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(83..1450)
                     /locus_tag="QIE41_002209"
     CDS             complement(83..1450)
                     /locus_tag="QIE41_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDS3531091.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVVG
                     GPIVAHILSSHYESYNLHIAELTNENDQVVWKTSYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDSLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVSIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
     gene            2042..4465
                     /gene="pgaA"
                     /locus_tag="QIE41_002210"
     CDS             2042..4465
                     /gene="pgaA"
                     /locus_tag="QIE41_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDS3531092.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAANNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLESQNKDYQRGQILTLADAGHYDSALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHEDELRAMTGSLPENALTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIIRDWLAGVEWRSRNIWLEAEYTERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            4474..6492
                     /gene="pgaB"
                     /locus_tag="QIE41_002211"
     CDS             4474..6492
                     /gene="pgaB"
                     /locus_tag="QIE41_002211"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDS3531093.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHQGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            6485..7810
                     /gene="pgaC"
                     /locus_tag="QIE41_002212"
     CDS             6485..7810
                     /gene="pgaC"
                     /locus_tag="QIE41_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDS3531094.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWAIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            7812..8225
                     /gene="pgaD"
                     /locus_tag="QIE41_002213"
     CDS             7812..8225
                     /gene="pgaD"
                     /locus_tag="QIE41_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDS3531095.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(8275..9063)
                     /gene="phoH"
                     /locus_tag="QIE41_002214"
     CDS             complement(8275..9063)
                     /gene="phoH"
                     /locus_tag="QIE41_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDS3531096.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(9683..10954)
                     /gene="efeB"
                     /locus_tag="QIE41_002215"
     CDS             complement(9683..10954)
                     /gene="efeB"
                     /locus_tag="QIE41_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDS3531097.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIASDNLTITLSVGHSLFDERFGLTPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQQVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(10960..12087)
                     /gene="efeO"
                     /locus_tag="QIE41_002216"
     CDS             complement(10960..12087)
                     /gene="efeO"
                     /locus_tag="QIE41_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDS3531098.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(12145..12975)
                     /locus_tag="QIE41_002217"
     CDS             complement(12145..12975)
                     /locus_tag="QIE41_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDS3531099.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(13629..15137)
                     /gene="putP"
                     /locus_tag="QIE41_002218"
     CDS             complement(13629..15137)
                     /gene="putP"
                     /locus_tag="QIE41_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDS3531100.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(15296..15505)
                     /locus_tag="QIE41_002219"
     CDS             complement(15296..15505)
                     /locus_tag="QIE41_002219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531101.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            15560..19522
                     /gene="putA"
                     /locus_tag="QIE41_002220"
     CDS             15560..19522
                     /gene="putA"
                     /locus_tag="QIE41_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDS3531102.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPIAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDTLHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(19562..20200)
                     /gene="rutR"
                     /locus_tag="QIE41_002221"
     CDS             complement(19562..20200)
                     /gene="rutR"
                     /locus_tag="QIE41_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDS3531103.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            20488..21579
                     /gene="rutA"
                     /locus_tag="QIE41_002222"
     CDS             20488..21579
                     /gene="rutA"
                     /locus_tag="QIE41_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDS3531104.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            21579..22271
                     /gene="rutB"
                     /locus_tag="QIE41_002223"
     CDS             21579..22271
                     /gene="rutB"
                     /locus_tag="QIE41_002223"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDS3531105.1"
                     /translation="MTILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            22283..22669
                     /gene="rutC"
                     /locus_tag="QIE41_002224"
     CDS             22283..22669
                     /gene="rutC"
                     /locus_tag="QIE41_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDS3531106.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            22677..23477
                     /gene="rutD"
                     /locus_tag="QIE41_002225"
     CDS             22677..23477
                     /gene="rutD"
                     /locus_tag="QIE41_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDS3531107.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVIC
                     YDQRGTGNNPDTLEEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASLTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            23487..24077
                     /locus_tag="QIE41_002226"
     CDS             23487..24077
                     /locus_tag="QIE41_002226"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDS3531108.1"
                     /translation="MNEAISPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSIQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            24088..24582
                     /gene="rutF"
                     /locus_tag="QIE41_002227"
     CDS             24088..24582
                     /gene="rutF"
                     /locus_tag="QIE41_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDS3531109.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            24603..25931
                     /gene="rutG"
                     /locus_tag="QIE41_002228"
     CDS             24603..25931
                     /gene="rutG"
                     /locus_tag="QIE41_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDS3531110.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(26014..26187)
                     /locus_tag="QIE41_002229"
     CDS             complement(26014..26187)
                     /locus_tag="QIE41_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDS3531111.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            26560..27156
                     /gene="wrbA"
                     /locus_tag="QIE41_002230"
     CDS             26560..27156
                     /gene="wrbA"
                     /locus_tag="QIE41_002230"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDS3531112.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            27177..27404
                     /locus_tag="QIE41_002231"
     CDS             27177..27404
                     /locus_tag="QIE41_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDS3531113.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(27442..28683)
                     /gene="agp"
                     /locus_tag="QIE41_002232"
     CDS             complement(27442..28683)
                     /gene="agp"
                     /locus_tag="QIE41_002232"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDS3531114.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(28975..>30041)
                     /locus_tag="QIE41_002233"
     CDS             complement(28975..>30041)
                     /locus_tag="QIE41_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531115.1"
                     /translation="ECESLEKEFEEMQNDNDLSLFMRTLRTNDTKIYSGVSGGMTYTI
                     QYVQDIDVVRVSLPGRASESITDFKGYYWYGFMEYIENINACDDVFSEYCLEDENISV
                     QPERLNTPGIFNLDAGIDLSGISFIQSEINKTYELKYAPVDGDGYCLLRAILVLKQHD
                     YSWALGSHKTQKEVYEEFIKIVDKPTIEALVDAAFCDLREDVKTLFGVDLQSDNKIQG
                     VGSLMSWSFLFFKKEFIDSCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRIKAVKNF
                     LAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPPELFAQA
                     YKLNNKEMNAQLDYLNRYAI"
BASE COUNT         7568 a         7653 c         7348 g         7472 t
ORIGIN      
        1 aaagtacgag cttttttttc gtatattgca tagaccgggg ggatgagaag ccccaggcgg
       61 aggtcgacaa ctagcgacag actcattcat catccttgcg gttgataaga atttctgcct
      121 cttttactac caccttatta cgcccggtct ctttcgcctc gtaaagtgcg ttatcagcca
      181 gttgatagat ctccttaggg tttaactcat ttttctgcgt tacaacagcg ccaatactaa
      241 tcgtcacttt ttgtggtatg gcgtattcag gattatcacc agttaaacgc tcaacatttt
      301 cccgaatcct ttccgctaaa gctttcgcac gttcagtatc gatgtccgtc agcaagacgc
      361 caaactcttc gccgccgacg cgcgcaaaaa ggtcatctgg ccgtatgctt ttcccaatga
      421 tactcaccac tgtttttatc acctgatcgc caaccggatg cccccaggtg tcgttgactt
      481 ttttgaagtg gtcgatatcc ataatcatga cgcaataagg cgtttttttg gccgatgctg
      541 attgaactgt cagttcatta aaaaaataat ttctgttaaa tatattggtt aaggaatcgc
      601 gatgtgcaat gtcctttgtt actcgtagcg cactgaatat atggcacata aatattacca
      661 taacggttaa cttactgact acttcaatag tgcgactaat ataccaaatg gacacgctat
      721 acctgctcat aaataaaaga gagacattgt acagtacggc acaaaaagca atcaccgtca
      781 ctccattcca gatatcacag cgtaaaccat taaagtatag attaactgaa agcaaggtta
      841 accacatgaa aatcattatg gtaacatatg atgttttcca aaccacctga tcattttcat
      901 tggttaactc ggcaatgtgt aaattgtagc tctcataatg actagaaagt atgtgagcaa
      961 ctatcggccc cccaacaaca aaaaacaaac ttatgcacaa agtcattttt ttggaaaata
     1021 aattgcgctg ttttgtgttt tcactaactt tattcaccaa tgccagaaat attaatatgc
     1081 acaaactcat ctgacgaaac aaataataga ttgcaatgtc attttgaaca acactgcttg
     1141 tcaaacgccc ctcaataatt tgctgaatga taatgacggt tttaacaaag taaatcaaac
     1201 aactcagaaa agccaaacta agtatacagt ttgaaaattg cctacggtca taaatgaaat
     1261 acaactgcat aaaaataaat gcaacagtat cgataaaaaa taaagcaatc attaccatca
     1321 gataggttga tcgtggaaca acttcctcaa cgcctggctg attaaaccaa ctattcacca
     1381 aaaccaaagc aagcccaaac aataagctca ctaatacagt actactaatt ctcaaatagt
     1441 ctttttccat aaagctacac caatctgtag gttgtagatc cctttctatt attaatagat
     1501 ataagaactg tactgttgtc aatatgaaat tccgcccaca aacaataccg atgatgatag
     1561 ttgcagactg caattattat taccttagtg ctttatacat tcttcgcgac aagacaggct
     1621 tgcaaataca tataaatggt tataaggaaa tccttataaa acattaattc atagatatat
     1681 taggaataac aattgaatcc gtgagtacag taacgctgca tttcatcagg actttcgaaa
     1741 aatccgatat catgcatcgg aatttactga tttaattatt ttaatcctaa tttattttga
     1801 aaaaggcatt gggatttatg ccgtattcct gaagttcctc atcattggaa tggattttcg
     1861 ggcgagaaaa ggattttata tggatactct actcatcatt tcttcttctc atcatcaaca
     1921 attcacgtct ctcttccgcg tttaataacg gattatgatg tgcaaaaaaa aatatctttc
     1981 gtttcagtta cctgtaatta gatacagaga gagattttag caatacatgg agtaatacag
     2041 gatgtattca agtagcagaa aaaggtgccc gaaaaccaaa tgggctttga aaattcttac
     2101 tgccgcattt ttagcagcga gtcccgcggc gaagagtgct gctaataatg cctatgatgc
     2161 attgattatc gaagctcgca agggaaatac tcagccagct ttgttatggt ttgcacaaaa
     2221 atcagcactc agtaataacc aaattgctga ctggttacag attgccttat gggccgggca
     2281 agataaacag gttattaccg tttacaaccg ctaccgtcat cagcaattac cagcgcgtgg
     2341 ttatgcagct gtcgccgtcg cttatcgtaa cctgcaacaa tggcaaaact cgcttacact
     2401 gtggcaaaag gcactctctc tggagtcgca aaataaggat tatcaacggg gacaaatttt
     2461 aaccctggca gatgcgggtc actatgattc tgcgctggtt aaacttaagc agcttaactc
     2521 tggagcaccg gacaaagcta atttactcgc agaagcctat atctataaac tggcggggcg
     2581 gcatgaggat gaattacggg cgatgacagg gtcattacct gaaaatgcct taacgcaaca
     2641 atatcccaca gaatacgtgc aggcattacg gaataatcaa cttgctgccg cgattgacga
     2701 tgccaattta acgccagata ttcgcgctga tattcatgcc gaactggtca gactgtcatt
     2761 tatgcctacg cgcagtgaaa gtgaacgtta tgccattgcc gatcgggccc tcgcccaata
     2821 cgctgcatta gaaattctgt ggcatgataa ccctgaccgc actgcccagt accagcgtat
     2881 tcaggttgat catcttggcg cgttattaac tcgcgatcgt tataaagacg ttatttctca
     2941 ctatcagcgt ttaaaaaaga cggggcaaat tattccgccc tgggcgcaat attgggttgc
     3001 gtcggcttat ctcaaagaac atcagccaaa aaaagcacag tcaataatga cagagctctt
     3061 ttatcacaag gagaccattg ccccggattt atccgatgaa gaacttgcgg atctctttta
     3121 cagccacctg gagagtgaaa attatcccgg cgcgctaact gtcacccaac ataccattaa
     3181 tacttcgccg cctttccttc ggttaatggg cacgcctacg agcatcccga atgatacctg
     3241 gttacagggg cattcgtttc tctcaaccgt agcaaaatat agtaatgatc ttcctcaggc
     3301 tgaaatgaca gccagagagc ttgcttataa cgcaccagga aatcagggac tgcgcattga
     3361 ttacgcgagt gtgttacaag cccgcggttg gcctcgtgcg gcagaaaatg aattaaaaaa
     3421 agcagaagtg atcgagccac gtaatattaa tctggaggtt gaacaagcct ggacagcatt
     3481 aacgttacaa gaatggcagc aggcagcggt cttaacgcac gatgttgtcg aacgtgaacc
     3541 gcaagatccc ggcgttgtac gtttaaaacg tgcggttgat gtacataatc ttgcagagct
     3601 tcgtatcgct ggctcaacag gaattgatgc cgaaggtccg gatagtggca aacatgatgt
     3661 cgacttaacc accatcgttt attcaccacc gctgaatgat aactggcgcg gttttgctgg
     3721 attcggttat gccgatggac aatttagcga aggaaaaggg attattcgcg actggcttgc
     3781 gggtgttgag tggcggtcac gtaatatctg gcttgaggcg gagtacaccg aacgcttttt
     3841 caatcatgag cataaacccg gcgcgcgcct gtctggctgg tatgatttta atgataactg
     3901 gcgtattggt tcgcaactgg aacgcctctc tcaccgcgtt ccattacggg caatgaaaaa
     3961 tggtgttaca ggcaacagtg cacaggctta tgttcgctgg tatcaaaatg agcggcgtaa
     4021 gtacggtgtc tcctgggctt tcactgattt ttctgacagt aatcagcgtc atgaagtctc
     4081 acttgagggt caggaacgca tctggtcttc accatatttg attgtcgatt tcctacccaa
     4141 tctgtattac gaacaaaata cagaacacga cactccatac tacaacccta taaaaacgtt
     4201 cgatattgtt ccggcatttg aggcaagtca tttgttatgg cgtagctatg aaaatagctg
     4261 ggagcaaatc ttcagcgcag gtgttggtgc ctcctggcaa aaacattatg gcacggatgt
     4321 cgtcacccaa ctcggctacg ggcaacgcat tagctggaat gacgtgattg atgctggcgc
     4381 aacgctacgc tgggaaaaac gaccttatga cggtgacaga gaacacaact tatacgttga
     4441 attcgatatg acattcagat tttaaggata aatatgttac gtaatggaaa taaatatctc
     4501 ctgatgctgg tgagtataat tatgctcact gcgtgcatta gccagtcaag aacatcattt
     4561 ataccgccac aggatcgcga atctttactc gccgagcaac cgtggccgca taatggtttt
     4621 gtggcgattt catggcataa cgttgaagac gaagctgccg accagcgttt tatgtcagtg
     4681 cggacatcag cactgcgtga acaatttgcc tggctgcgcg agaacggtta tcaaccggtc
     4741 agtattgctc aaattcgtga agcacatcaa ggaggaaagc cgctaccgga aaaagcagta
     4801 gtgctaactt ttgatgacgg ctaccagagt ttttataccc gcgtcttccc aattcttcag
     4861 gccttccagt ggcctgctgt atgggccccc gtcggcagtt gggtcgatac gccagcggat
     4921 gaacaagtaa aatttggcga tgagatggtc gatcgagaat attttgccac gtggcaacaa
     4981 gtgcgagaag ttgcgcgttc ccggctcgtt gaggtcgctt ctcatacatg gaattctcac
     5041 tacggtattc aggctaatgc caccggcagc ttattgcctg tatatgtaaa tcgtgcatat
     5101 tttactgacc acgcacggta tgaaaccgca gcagaatacc gggaaagaat tcgtctggat
     5161 gctgtaaaaa tgacggaata cctgcgtaca aaggctaagg taaatccaca cgtttttgtt
     5221 tggccttatg gcgaagcgaa tggcatagcg atagaggaat taaaaaaact cggttatgac
     5281 atgttcttca cccttgaatc aggtttggca aatgcgtcac aattggattc cattccgcga
     5341 gtattaatcg ccaataatcc ctcattaaaa gagtttgccc agcaaattat taccgtacag
     5401 gaaaaatcac cacaacggat aatgcatatc gatcttgatt acgtttatga cgaaaaccgc
     5461 cagcaaatgg atcgcaatat tgatgtgcta attcagcggg tgaaagatat gcaaatatca
     5521 accgtgtatt tgcaggcatt tgctgatccc gatggtgatg ggctggtcaa agaggtctgg
     5581 tttccaaatc gtttgctacc aatgaaagca gatattttta gtcgggttgc ctggcaatta
     5641 cgtacccgct caggtgtaaa catctatgcg tggatgccgg tattaagctg ggatttagat
     5701 cccacattaa cgcgagtaaa atacttaccg actggggaga aaaaagcaca aattcatcct
     5761 gaacaatatc gccgtctctc tcctttcgat gacagagtca gagcacaagt tggcatgtta
     5821 tatgaagatc ttgccggaca tgctgctttt gatggcatat tgttccacga tgatgctttg
     5881 ctttcagatt atgaagatgc cagtgcaccg gctatcacgg cttatcagca agcaggcttt
     5941 agcgggagtc tgagcgaaat tcgacaaaac ccggagcaat ttaaacagtg gacccgcttt
     6001 aaaagtcgtg cgttaactga cttcacttta gaacttagtg cgcgcgtaaa agccattcgc
     6061 ggtccacatg ttaaaactgc acgaaatatt tttgcacttc cggtaataca acctgaaagt
     6121 gaagcctggt ttgcacagaa ttatgctgat ttcctaaaaa gctatgactg gaccgctatt
     6181 atggctatgc cttatatgga aggtgtcgca gaaaaatcgg ctgaccaatg gttaatacaa
     6241 ttgaccaatc aaattaaaaa catccctcag gctaaagaca aatctatttt agaattacag
     6301 gcacaaaact ggcagaaaaa tggtcagcat caggctattt cttcgcaaca actcgctcac
     6361 tggatgagcc tattacaact gaatggagtg aaaaactatg gttattatcc cgacaatttt
     6421 ctgcataacc aacctgaaat agacgttatt cgtcctgagt tttcaacagc ctggtatccg
     6481 aaaaatgatt aatcgcatcg tatcgttttt tatattatgt ctggtgttat gcatacccct
     6541 atgcgtagcg tactttcact ctggtgaact gatgatgagg ttcgttttct tctggccgtt
     6601 ttttatgtcc atcatgtgga ttgttggcgg cgtctatttc tgggtctatc gtgaacgcca
     6661 ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa gataatccgt ctatctccat
     6721 tatcattccc tgttttaatg aggagaaaaa cgttgaggaa accatacacg ccgctttagc
     6781 acagcgttat gagaacattg aagttattgc cgtaaatgac ggttcaacag acaaaacccg
     6841 tgccatcctg gatcgcatgg ctgcacaaat tccccacctg cgggtcattc atctggcgca
     6901 aaaccagggg aaagccattg cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct
     6961 ggtgtgcatt gatggcgatg cgttattaga ccgcgatgcg gcggcatata ttgtggaacc
     7021 gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat cctcgtattc gaacacgttc
     7081 taccctggtg ggtaaaattc aggttggcga gtattcctca attattggtt tgatcaagcg
     7141 aacccagcgt atctatggaa acgtatttac cgtttccggt gttattgccg catttcgtcg
     7201 cagcgccctg gcagaagtgg gttactggag tgacgatatg atcaccgaag atattgatat
     7261 tagctggaag ctgcagttga atcagtgggc gattttttac gagccacggg cgctgtgctg
     7321 gatattaatg cctgaaacgt taaaagggct gtggaaacag cgcctgcgct gggctcaggg
     7381 cggtgcagaa gtattcctca aaaatatgac aaggttgtgg cgcaaagaaa actttcgaat
     7441 gtggccgctg ttttttgaat actgcctgac gacaatatgg gccttcactt gcctggtcgg
     7501 tttcattatt tacgcagtcc aacttgccgg tgtaccgtta aatattgaat tgacacatat
     7561 cgctgcgaca catactgccg gaatattatt gtgtacgtta tgtttactgc aatttattgt
     7621 cagcctgatg atcgagaatc gctatgagca taatctgact tcatcgcttt tctggattat
     7681 ttggttcccg gttattttct ggatgctgag tctggcaacg acattggtat catttacacg
     7741 agtcatgttg atgcctaaaa agcaacgcgc ccgttgggta agtcccgatc gcgggattct
     7801 gagaggttaa tatgaacaat ttaattatta cgacccgaca atcgccagta cgtttactgg
     7861 ttgattatgt tgccacaacc atcttatgga cattatttgc tttgttcata ttcttattcg
     7921 caatggatct gctgacgggt tattactggc aaagcgaggc cagaagccga cttcagttct
     7981 attttttgct ggcagtggcg aatgccgtcg tgttaattgt ctgggcgctg tacaataagc
     8041 tacgttttca aaaacagcag catcatgcag cctaccaata tacgccgcaa gaatatgctg
     8101 agagcttagc aatacctgat gagctctatc agcaactaca aaaaagccac aggatgagcg
     8161 tacacttcac cagccagggg caaataaaaa tggtggtttc agaaaaagcg ctagtccggg
     8221 cgtaaacacc caaaacaaag cccggttcgc ccgggctctg cgccgataat acacttaact
     8281 gtatgcatgt agcgtacgtt ggcaaagtgc cgaacgtacg caatcctctt taccgaatcg
     8341 gacgatcccg accatttcat cttcttcgaa acgttccagc gcgtcactta atccggagcg
     8401 cacgccgcga ggcaaatcgc attgcgtgat atcaccgttg acgataaccg tcacgttctc
     8461 cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc acattctgcg cctcgtcaag
     8521 aatgacgact gcattttcaa aggtacgtcc acgcatatag gcgaacggcg caatttccac
     8581 cttccctatt tccggtcgca ggcagtactg cataaaggaa gcccctaagc gccggaccag
     8641 cacgtcgtag accggacgaa aatagggagc aaacttttct gcgatatcac caggtaagaa
     8701 gccaagatct tcatcggctt gcagaactgg acgggtgacg ataatcctgt cgacatcctt
     8761 atgtatcagg gcctctgccg cttttgctgc gctgatccag gtttttccgc acccggcttc
     8821 gcccgtggcg aatatcagct gcttactctc aatagccttc agatagtgca attgcgcttc
     8881 atttcgcgcg aggatgggcg aggtatcgcg actgtcgcgg gccataccaa tggcttctac
     8941 gccgctcatc tgcacaagcg aggtgaccga ttcttcttca cgctgcttat ggctgcgcga
     9001 atcccgtctc agcacacgtt ttgcctcgcg acgagctttg atcactgctt tttgtcttcc
     9061 catggagagc accttgagtt gtttgtattc atcacacgcg ccgttggcag cgcgattatg
     9121 cgcacgaaca tcagagggtt ggcttccttg taagccatag tttgcttttg gataaaatgc
     9181 cgaaaaacgg ctacgcacac cgtttgcggc gtcggtaaca cgagtaaaga gaggaagtga
     9241 gaaaaataca gagtgacgaa gttgctaccg gaggagaaat ctccgcgagt ggtggagcct
     9301 tgcttaacaa aacattgtcc agaacaggac gttaccatcc gcgatctcca tagtgactga
     9361 ctatcactgc cgggaacttc cgctgctact taataagtac aacagatctc gcattcattg
     9421 caacaatata ttcacttata tttaactaca aaacaccatt tcagtgacat tagtttctac
     9481 tggaaagatg acagagtgat gacagtgatg aaaaaagctg tgtgctttca gcaggattcg
     9541 catcaggcat cagcgcacga acgccggatg cggcgtgaac gcctgatcca gcctaccaaa
     9601 cccgcaccga ttgtaggcat gataagacgc gcaagcgtcg catcaggcat ctgcgcacga
     9661 acgccttatc cgcctaaaca cattaaaccc gcaataacgc gcttccgaga taatcgttcg
     9721 cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta acgtattcct
     9781 ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc agatcgtgtt
     9841 gatagcagac aaacagtagc cccatatcca gttgtccgga gttggtgacg cccaacgaat
     9901 aactgtagcc acgacgcagc atcaggctgg actcactctc tggcgtgcgg ggattcgcca
     9961 gccggatatg gctgtccagc gcaatcacct tcccctccgg atcgctggcg taatcgggaa
    10021 catcatgctc atgctgcatt cccagcggcg caccggtttg tttgtcgcga ccaaaaatcg
    10081 tctgctgttc tttcagtggc gttctgtccc aaaactccac ccgaaactga atcaaccgca
    10141 ccgcctggta gctgccgccg attgtccacg caggctcctg ctgatctgcc gtcacccaca
    10201 ccacttgttg catcaactta tcattctggc tatcgggatt ggcagtgccg tctttgaaac
    10261 ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc tgcatgatcg gaaataaacc
    10321 cttcccgctt ccagcgcacg ctgagcaaat ccggcgtgtg tttgatgata tcgcgcagcg
    10381 catggatcac cgtgtcctgg gtgttggcgc aaatttgtag caacacatca ccatgacata
    10441 acgccgcatc cagcgagtcg ttggggaaac gcgtcatttt ctgcaacttt ttcggcatct
    10501 gcggtgtaag gccaaaccgc tcatcaaaca atgagtggcc caccgataac gttatggtga
    10561 gattatcgga cgcaatgtag ccgccaagaa tgccggaatc gagtggaggc aggcgcggat
    10621 ttggcgtttc tggtgccgct ccgccctgag tcagaaaagc aaaacgctga gtcaacaagc
    10681 gaaacaacct ttcaagatcg gctttatcgc tggcaagcac atcaaacgcc accagcatca
    10741 ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac ggctgtttct
    10801 cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt gcatgagcga
    10861 ccggacaact tcccgccagc gccagcgcac caatcccttt cagtaaacgt cggcgtgacg
    10921 gttcattcac gccgttttca tcttcatact gcataacgtt taatccagcc ccagcacacc
    10981 gcgaagttgc gccagatctt ccgccagcgc agtaatcggc cctttcagcg cattacggtc
    11041 agcatcggtc aatttgtcgt aggtttcaaa accgtcttta gtacggtatt tcgccagaat
    11101 ggtatcgacc tttttgaaat tggcatcgac ttttgccagc agttccgggt tggccttttg
    11161 cagttgtgga cgcagcagat cgacaatttt ctgcgagcct tcaacgttag cctggaaatc
    11221 ccacagatcg gtgtggctgt agcgatcttc ttcaccgctg attttgctgg ctgccacttc
    11281 ctcaatcagt ccggctgcac cgccgaccac ttttgaaggt gggaaagcca gttcactgat
    11341 gcgtttttgc aaatcgacca catcggtata aagctggtcg gcatacttat ccatcccttt
    11401 agtggtgtta tcgccaaaca atgctttttc cagacggtgg aaaccggtga attttggatc
    11461 ggcggctttt tgctcgtaat catcttcacg ggcgtcaatg ctaccatcca gatcggagaa
    11521 cagttcagca atcggttcaa tgcgctcata gtgctggcgc gtcggcgcat acagtgcttt
    11581 tgctttttcg atatcgcctg ctttaatagc gtcggtaaag gctttggtgt cggtcaccag
    11641 ctgcgtggtt tccgccatga catacgcttt atatgcagta attgcaccac caagacttaa
    11701 cagcgcatca ctttgcgccg catccgccgt tgcctcacct ttgacgatca acttcccttt
    11761 cgggttagtc agcagaccgc aggtcatatc gtattcgcca ggctgtaaat tcgccgtcat
    11821 tttctggcta aaaccagggg cgatattttc ccgctcttcc accaccatca cgcctttgag
    11881 gatctcccac tccagcgcct tctggctgtg gttctgaata atgaactgtg ttttcccggc
    11941 gttaaccgta atggtcatcg gttcgcactg cttatccgtc acggtcactt tgacctgcgg
    12001 cacatcagcg gcgttagcca taaaagcaga agaaaacagc gcagccacgc tcaactgcaa
    12061 tgcgttacgg cggaagttaa tggtcatgac gagactatcc ctttaaagta tgttgtaact
    12121 aagcaagagt ttgcgtcgta tttgctacgc agagcgagac gctgtcgccc ctgcgcgtgg
    12181 tggcagagca aatgccacca gcgccgggat gagataaata aaccagacgg cgacttcgct
    12241 gacacttggc gcttcctgat agccaaaaat cccttccatc agcgtgccaa acagcgagtg
    12301 agttgagagc accgcactca tatcgaaggc gatttcctga aagtggttcc acaatccggc
    12361 ttcatgaaat gcgcgaatgg caccagccgc cagccctgcg gcgacgaaga gaataaacag
    12421 gctggtccat ttaaaaaatg caccaagatt gaggcgaata ccgccccagt agagcaggaa
    12481 gccgagcacc acggctgtag caagaccgag cattgcaccc agcggcggcc agatcccgac
    12541 atcttgttga aacgccgcca gcaggaaaaa gaccgactcc agcccctccc ttgcaacggc
    12601 aaaaaagacc atcatcacca gcgcccagcc atgatgattt ccacgctgca atgcgctatc
    12661 gactgcctgc tccagttgta ctttgacgtt gcgcgacact ttgcgcatcc agaaaaccat
    12721 ccaggtaagg atcaccacgg cgatcaccgc cacgatacct tcaaacagtt cctgttcttt
    12781 ttgcggaaat tcgccggtgg tttcgttaat gaagataccc aagcccaggc acaacgcagc
    12841 ggcaagcaac acgccaatcc acatcacacc aatccatcgg cctcgctggg tacgcttaag
    12901 atagctggcg atcaaactga caatcagcgc ggcttcaagt ccttcgcgca acataatgag
    12961 aaatggaaca aacatgccac ccacccttaa acgtttttct gagtcaatct atgcaaagaa
    13021 aagtaaatcg cagtgatagc gattatcatt acggcaaaga aaaatacaag cggaatgttt
    13081 ataaaatgat gttggaatgt aaacaaagag aggtaatgcc aggggatata aacgattatt
    13141 ccaggtacaa agatgggatt gtttacaacg agccatgtgt tcttgccaga tgccgcgtaa
    13201 acgccttatc cagccgacaa aactgtccag actcaatgca tcagagcttc catgtaaaac
    13261 tgataagcgg gtgtatcaag caatttcgtc catccgcgat tatcaaatct aactttttgc
    13321 ccggaacata ataacagctc gggtgttggt ggcgtcaaag tcgctgaagc gttccccgaa
    13381 ggccggggca gcccacatgg atgtgggctg agggcgcgtc ttacaggaca ttacctcgcg
    13441 cccgacccgg tagccgaagg ggataagctg aaggtaccgc gcagcggcga ttttgttcgc
    13501 cagagcccgg ggtgcagggg gtggcggcga ttggccgccc cctgcaccat tcaatttata
    13561 tggcgaggca cattaaggga gcgcaacccg ctgctgaagc caaacaaaac cgccgcaggc
    13621 taagtccctt agctttcctg caaccgtgac ggcggagccg aatgatagtg cgcatcggcc
    13681 tcggcaaagc gtttttgcat cgccgctgac ggcgctttac ccagcaaact aaacactaca
    13741 atcccgatac taccaaagat aaagcccgga ataatttcgt acagacccag ccagccgaac
    13801 tgtttccaga cgataaccgt cagcgcaccg atgatcatcc ccgccagcgc accgttgcgc
    13861 gtcatgcgtg accacatcac cgagaacagc accaccggac caaacgccgc gccaaagcct
    13921 gcccacgcgt agctcactaa gcccagcacg cggttttccg ggtttgccgc cagcgcaatg
    13981 gccaccagcg ccaccaccag caccatcaca cgccctaccc acaccagctc tttctggctg
    14041 gcctgtttac gcagaaacgc tttgtataaa tcttcggtaa tcgcactgga gcacaccagc
    14101 agctggcaac ttaaggttga cattaccgcc gccagaatcg ccgacagcag aatcccggca
    14161 atccacgggt taaacagaat ttgcgccagt tcgataaaca cacgttcagc attctggttt
    14221 accgcacctg ctaccgacgg gtgctcatta aagtaagcaa taccaaagaa gccgacagcc
    14281 accgcccctg ccaggcagag gatcatccag gtcatactga tacgacgcgc atggacaatg
    14341 ctgtggtgag aatccgccgc cataaaacgc gccaggatgt gcggctgccc gaagtaaccc
    14401 agcccccagc ccatcagcga gataatggca acaaagttca gtcctttgag catatcgacg
    14461 ttttcgatgc tcttttgttt gatcacttcc agcgagtcgc caaacccacc aacgctaatg
    14521 attacgataa ccggcgtcag gatcagggca aaaatcatca ggctggcctg tacagtgtca
    14581 gtccagctca ctgcaaggaa gccaccaata aaggtgtaaa ggatcgtcgc cgcagcgccc
    14641 gcccacagag ccgtttcgta gctcatgcca aaggtacttt caaacagacg cgcgcctgcc
    14701 acgatgcccg aagcgcaata aatggtgaag aacagcaaaa tcaccagcgc ggagataatg
    14761 cgcaaaatac ggcttttatc ttcaaagcgc ccggtaaaat aatccggcaa tgttaaggcg
    14821 ttattgttgt attcggtatg cacacgcaac cgcccggcca ccagcttcca gttaatccac
    14881 gcgcctaatg tcaggccaat ggcgatccag ctttcggaaa tcccggaaag aaaaaccgcg
    14941 cccggcaacc ccattaacag ccagccgctc atatccgatg cacccgccga taatgccgtc
    15001 acgaatggcc caaggctacg tccgcccaga atatagtcgt caaagttttt cgttgatcgc
    15061 caggcgataa acccaatcaa tatcatgcca aagatataga cacaaaatgt caccaacatc
    15121 ggtgtgctaa tagccatcta aagtctccaa aaaattatta tcggcaatgt cgaaacttgc
    15181 cgttatatct gccaccggaa cggggtagca gagtttatgt tttaccaggg cgaccgtatc
    15241 ctgccggaag cgctggttat tcacaatcga tttaacacac catttacatt aaattttagt
    15301 gctcagcgac actatttttc atcaggttgc actctctcac attttttgcg gttgcacctt
    15361 tcaaaaatgt taactgccgc agagaaaaag tctgagttat ttttttaatc cctgtcatat
    15421 cgatttcttt tattaacatt tcattcattt ttaagcttgc tacgcaggtc acatttaaca
    15481 tggttgcaca aagttgcaac atcatggata tttcacgata acgttaagtt gcacctttca
    15541 gaacaacagg agtaatggca tgggaaccac caccatgggg gttaagctgg acgacgcgac
    15601 gcgtgagcgt attaagtctg ccgcgacacg tatcgatcgc acgccacact ggttaattaa
    15661 gcaggcgatt ttttcttatc ttgaacaact ggaaaacagc gatactcttc cggagctacc
    15721 tgcgctgctc tctggcgctg ccaatgagag cgatgaagca ccgactccgg cagaggaacc
    15781 acaccagcct ttcctcgact ttgccgagca aattttgccc cagtcggttt cccgcgccgc
    15841 gatcaccgct gcctatcgcc gcccggaaac cgaagcggtt tctatgttgc tggaacaagc
    15901 tcgcctgccg cagccgattg ctgaacaggc gcataaactg gcatatcaac tggccgataa
    15961 actgcgtaat caaaaaaatg ccagtggtcg cgcaggtatg gtccaggggt tattgcagga
    16021 gttttcgctg tcatcgcagg aaggcgtggc gctgatgtgt ctggcagaag cgttgttgcg
    16081 tattcccgac aaagccaccc gcgacgcgtt aattcgcgac aaaatcagca acggtaactg
    16141 gcagtcacac attggtcgta gcccgtcgtt gtttgttaat gccgccacct gggggctgct
    16201 gtttactggc aaactggttt ccacccataa cgaagccagc ctctcccgct cgctgaaccg
    16261 cattatcggt aagagcggtg aaccgctgat ccgcaaaggt gtggatatgg cgatgcgcct
    16321 gatgggcgag cagttcgtca ctggcgaaac catcgcggaa gcgttagcca atgcccgcaa
    16381 gctggaagag aaaggtttcc gttactctta cgacatgctg ggcgaagccg cgctgactgc
    16441 cgcagatgca caggcgtata tggtttccta tcagcaggcg attcacgcca ttggtaaagc
    16501 gtccaatggt cgtggcatct atgaaggtcc gggcatttca atcaagctgt cggcgctgca
    16561 tccgcgatac agccgcgccc agtatgatcg ggtaatggaa gagctttacc cgcgtctgaa
    16621 atcactcacc ctgctggcgc gtcagtacga tattggtatt aacatcgatg ccgaagaggc
    16681 cgatcgtctg gagatctccc tcgatctgct ggaaaaactc tgtttcgagc cggaactggc
    16741 aggctggaac ggcatcggtt ttgttattca ggcttatcaa aaacgctgcc cactggtgat
    16801 tgattacctg attgatctcg ccacccgcag ccgtcgccgt ctgatgattc gtctggtgaa
    16861 aggcgcgtac tgggatagtg aaatcaaacg tgcgcagatg gacggccttg aaggttatcc
    16921 ggtttatact cgcaaggtgt ataccgacgt ttcttatctt gcctgtgcga aaaagctgct
    16981 ggcggtgcca aatctaatct acccgcagtt cgcaacgcat aacgcccata cgctggcggc
    17041 aatttatcaa ctggcgggtc agaactacta cccgggtcag tacgagttcc agtgcctgca
    17101 cggtatgggc gagccactgt atgagcaggt caccgggaaa gttgccgacg gcaaacttaa
    17161 ccgtccgtgt cgtatttatg ctcctgtcgg tacccatgaa acgctgctgg cgtatctggt
    17221 gcgtcgcctg ctggaaaacg gtgctaacac ctcgtttgtt aaccgtattg ctgacacctc
    17281 tttgccactg gatgaactgg ttgccgatcc ggtcactgct gtagaaaaac tggcgcaaca
    17341 ggaagggcaa actggattac cacatccgaa aattcccctg ccgcgcgatc tttacggtca
    17401 cgggcgcgac aactcggcag ggctggatct cgctaacgaa caccgtctgg catcgctctc
    17461 ttctgccctg ctcaatagtg cactgcaaaa atggcaggcc ttgccaatgc tggaacaatc
    17521 ggtcgcggct ggtgagatgt cgcccgttat taaccctgcg gaaccgaaag atattgtggg
    17581 ctttgtgcgt gaagccacgc cgcgtgaagt agaacaggcg ctggaaagtg cggttaataa
    17641 cgcgccaatc tggtttgcca cgcctccggc tgaacgcgca gcgattttgc accgcgctgc
    17701 cgtgctgatg gaaagccaga tgcagcaact gattggtatt ctggtacgtg aggccggaaa
    17761 aaccttcagt aacgccattg ccgaagtgcg cgaagcggtc gattttctcc actactacgc
    17821 cggacaggtg cgggatgatt tcgctaacga aacccaccgt ccattagggc ctgtggtgtg
    17881 tatcagtccg tggaacttcc cgctggcgat tttcaccggg cagattgctg ctgcactggc
    17941 ggcaggtaac agcgtgctgg caaaaccggc agaacaaacg ccgctgattg ccgcgcaagg
    18001 gatcgccatt ttgctggaag cgggtgtacc gccaggcgtg gtgcaattgc tgccaggtca
    18061 gggtgaaacc gtgggcgcgc aactgacggg tgatgatcgc gtgcgcgggg tgatgtttac
    18121 cggttcaacc gaagtcgcta cgttactgca acgcaatatc gccagccgcc tcgacgctca
    18181 gggtcgccct attccgctca tcgcggaaac cggcggtatg aacgcgatga ttgtcgactc
    18241 ctccgccctg accgaacaag tagtaataga tgtgctggcc tcagcgttcg acagtgcggg
    18301 ccagcgttgt tcggcactgc gcgtgctgtg cctgcaagat gagattgccg accacacgtt
    18361 gaaaatgctg cgcggcgcaa tggccgaatg ccggatgggt aatcctggtc gcctgaccac
    18421 cgatatcggt ccggtgattg atagcgaagc gaaagccaat attgagcgtc atattcagac
    18481 tatgcgtagt aaaggccgtc cggtgttcca ggcagtgcgg gaaaacagtg aagatgcccg
    18541 tgaatggcaa agcggcacct ttgtcgcccc gacgctgatt gaactggatg actttgccga
    18601 attgcaaaaa gaggtctttg gtccggtgct gcatgtggtg cgttataacc gtaaccagct
    18661 gccggagctg atcgagcaaa ttaacgcttc cggttatggt ctgacgcttg gcgtccatac
    18721 gcgcattgat gaaaccatcg cccaggtcac tggctcggcc catgtcggta acctgtacgt
    18781 taaccgtaat atggtgggcg cagtggtcgg ggtgcagcca ttcggcggcg aagggttgtc
    18841 cggcaccggg ccgaaagcag gcggtccgct ctatctctac cgtctgctgg cgaatcgccc
    18901 ggaaagtgca ctggcagtga cgctcgcgcg tcaggatgca gagtatccgg tcgatgcgca
    18961 gttgaaagcc gcattgactc agccgctaaa tgcactgcgg gaatgggcgg caaaccgtcc
    19021 agaattgcag gcgttatgta cgcaatatgg cgagctggcg caggcaggaa cacaacgatt
    19081 gctgccaggg ccgacgggtg aacgcaacac ctggacgctg ctgccgcgtg agcgcgtgtt
    19141 gtgtattgcg gatgatgaac aggatgcgct gactcagctc gccgccgtgc tggcggtggg
    19201 cagccaggta ctgtggccgg atgacacgct gcatcgtcag ttagtgaagg cattgccatc
    19261 ggcagtcagc gaacgtattc aactggcgaa agcggaaaat ataaccgctc aaccctttga
    19321 tgcggtgatc ttccacggtg attcggatca gcttcgcgca ttgtgtgaag ccgttgccgc
    19381 gcgggatggc gcaattgttt cggtgcaggg ttttgcccga ggcgaaagca atatccttct
    19441 ggaacggctg tatatcgaac gctcgctgag tgtgaatacc gctgccgctg gcggtaacgc
    19501 cagtttaatg acgatcggtt aaacaggtag ccggaggatg ttacaacctc ctccggcatc
    19561 tttaacgtgg tcgaatcccc tcaataataa tccgctgcac gttttcaacc gtttgattga
    19621 aaaatacctc atcgcgcaac gtcgcgcctg tcaccgcctc cacctgaggg gcgaaatcgg
    19681 cgtaatgttg agtggaagcc caaatcataa aaatcaaatg ctgcgggtca atcggcgcga
    19741 gtttgccgct ttttacccaa ccggcaatca gcgccgattt ctcatcaatt aatgccttca
    19801 aatcgcccgt cagttcatcc attaacagcg gcgcgcctgc cagcatctcc atacagaaca
    19861 ggcgcgaagc ctgcggataa tcgcgtgaga cttccagctt cagacggatg tactctttga
    19921 tcgccgccag cggggcgaaa tcttcacgaa acgcttttaa cggtgccagc cagatatcga
    19981 gaatctgccg cagcacggca atatacagcg cctcttttga cggaaagtaa tacagcagat
    20041 tggtttttga aacacccgcc aactctgcga tctgctccag ccttgtgccg tgaaaaccga
    20101 attgtgaaaa agtgtccagt gctgcgctaa gaatcgcttt tttcttcgcg ctcactgcgc
    20161 gcgaacgttt acccgttgtt ttcactgcgc cttgcgtcat gcgctctccc ctcttgtaga
    20221 ttggcaacag gatagcaaaa cccctgcccg gcctcgacct tctgcacttt catcgcgcac
    20281 ggatgcatgt tttatgtgca actgttttga ccgtttagtc cacttttttc cagatatttt
    20341 agacaactta acattaacat attaataaca ttcacttttt aaaaactggc atccgctttg
    20401 caaacaaacc attacgcggc tgaaaaggat atgcaggatg cagcgccccg tctgactttc
    20461 actttacgag acgaagagag gttgatgatg aaaattggcg tattcgtacc tattggcaac
    20521 aacggctggc tcatttcgac ccacgcgccg cagtacatgc cgacctttga actgaataaa
    20581 gccattgtgc aaaaagcgga gcactaccat ttcgatttcg ccctgtcgat gatcaaactg
    20641 cgtggctttg gcggcaaaac tgagttctgg gatcacaacc ttgagtcgtt caccttgatg
    20701 gcggggctgg cagccgtgac ctcgcgcatt cagatatacg cgacagctgc caccttaacg
    20761 ttacctccgg caatcgtcgc ccgtatggcc gcaaccattg actccatctc tggcgggcgt
    20821 tttggcgtca acctcgtgac tggctggcaa aagcccgagt atgagcagat gggtatctgg
    20881 cctggcgatg actatttctc ccgtcgttac gactatctca ccgaatatgt tcaggtgctg
    20941 cgcgacctgt ggggctcggg gaaaagcgat tttaaaggcg attttttcac tatggatgat
    21001 tgtcgcgtca gtccgcaacc gagtgtcccc atgaaagtga tctgcgccgg gcaaagcgac
    21061 gctggcatgg cgttctccgc ccggtatgcc gattttaact tctgtttcgg caaaggcgta
    21121 aatacaccca cggctttcgc cccgaccgct gcgcggatga aacaggccgc agagcaaacc
    21181 ggacgcgacg ttggctctta tgtgttgttt atggtgattg ccgatgaaac cgacgatgcc
    21241 gctcgcgcca aatgggaaca ctacaaagcg ggcgcggatg aagaggcgtt aagctggcta
    21301 accgaacaaa gtcagaaaga tacccgctcc ggtactgaca ccaacgtccg tcagatggcc
    21361 gatcccactt cggcggtaaa catcaatatg gggacgttag tcggttctta cgccagtgtc
    21421 gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgccg aaggcgtgct attgaccttc
    21481 gatgattttc tgtcgggaat cgaaaccttc ggcgagcgca ttcaaccact gatgcagtgc
    21541 cgcgcccatc tccctgcgct gactcaggag gtggcatgat gacaatctta accgctcgac
    21601 cggaagccat taccttcgat ccgcagcaaa gtgcgctgat tgtggtggat atgcaaaacg
    21661 cttatgccac gccaggcggc tacttagatc tcgccgggtt tgatgtctca accactcgcc
    21721 cggtcattgc caacattcaa accgccgtga ccgcagcgcg agcggcaggg atgttgatta
    21781 tctggtttca aaatggctgg gatgaacagt atgtcgaggc tggcggcccc ggctcaccaa
    21841 attttcataa atcgaacgcc ctgaaaacca tgcgtaagca gccgcagcta caggggaaat
    21901 tgctggcgaa aggctcctgg gattatcaac tggtggatga actggtgccg cagcctggcg
    21961 atattgtgct gcctaagccg cgctacagcg gtttcttcaa tacgccgctg gacagcattt
    22021 tgcgcagccg cggaatacgc catctggttt tcaccggtat cgctaccaac gtctgcgtcg
    22081 aatcgacgct acgcgacggc ttttttctgg agtatttcgg cgtggtgctg gaagacgcaa
    22141 cacaccaggc ggggccggaa tttgcacaga aggccgcgtt gttcaatatc gaaacctttt
    22201 ttggctgggt cagcgacgtc gaaacgttct gcgacgcgct ttctcccacg tcctttgctc
    22261 gtatcgctta aggagtttaa cgatgccaaa atccgtaatt attcccgctg gcagcagcgc
    22321 accgctggct cccttcgttc ccggcacgct ggctgatggc gtggtgtatg tctccggtac
    22381 gctggctttt gatcaacata ataacgtgct gtttgccgat gacccaaagg cgcaaacccg
    22441 ccacgttctg gaaaccatcc gcaaggtgat cgagacggcg ggtggcacga tggcggatgt
    22501 gaccttcaac agcatcttta ttaccgactg gaaaaattac gccgcgatta acgaaatcta
    22561 cgccgagttt ttcccgggtg acaaaccggc gcgattctgc attcagtgcg gactggtaaa
    22621 acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc gccaagtgag gccgcgatga
    22681 aactttcact ctcacctccc ccttatgctg atgcgcccgt agtggtgttg atttcgggtc
    22741 ttggcggtag cggcagttac tggttaccgc aactggcggt actggagcag gagtatcagg
    22801 taatctgtta cgaccagcgc ggcaccggca ataatcccga cacgctggaa gaagattaca
    22861 gtatcgccca gatggcagcg gaactgcatc aggcgctggt agccgcaggg attgagcgtt
    22921 acgcggtggt cggccatgcg ctcggtgcac tggtgggaat gcagctggcg ctggattatc
    22981 ccgcgtcgtt aactgtgctg gtcagcgtta acggctggct acgaataaac gcccatacgc
    23041 gccgctgttt tcaggttcgc gagcagttac tacatagcgg cggcgcgcag gcatgggtgg
    23101 aagctcagcc attgttcctc tatcccgccg actggatggc ggcccgcgca cctcgcctgg
    23161 aagctgaaga cgcgctggca ctggcgcatt ttcagggcaa aaataattta ctgcgtcgac
    23221 ttaacgccct caaacgcgct gactttagtc accatgcgga tcgcatccgc tgcccggtgc
    23281 aaatcatctg cgccagtgat gatctgctgg tgccaacagc atgttccagt gaacttcatg
    23341 ccgccctgcc cgatagccag aaaatggtga tgcgctatgg cggacacgcc tgcaacgtga
    23401 ccgatcccga aacgtttaat gctctgttac tcaacgggct tgccagcctg ttacatcacc
    23461 gtgaagccgc cctgtaagga attgctatga acgaagccat tagcccaggt gcgcttagca
    23521 ccctgttcac cgatgcccgc actcacaacg gctggcggga gacacccgtc agcgatgaga
    23581 cgttacggga gctttatgcc ctgatgaaat gggggccgac atcagctaac tgttctccgg
    23641 cacggatcgt gtttatccgc actgcagaag gaaaagaacg tctgcgcccg gcgctttcca
    23701 gcggcaatct gcaaaaaacc ctgaccgcgc ccgtcaccgc gatcgtcgcc tgggacagtg
    23761 aattttatga acggttacca cagctgtttc ctcacggcga tgcccgcagt tggtttacct
    23821 ccagcccaca acttgccgaa gaaacagcgt ttcgcaacag ttccatacag gcggcctatc
    23881 tgatcgtcgc ctgccgggcg ctggggctgg ataccggccc gatgtccggc tttgaccgtc
    23941 aacacgtgga cgacgccttt tttacgggca gcacgctgaa gagcaatctg ctgattaata
    24001 tcggctatgg cgatagcagc aagctttatg cgcgcctgcc acgtctgtcc tttgaagaag
    24061 cctgcgggct gttgtaagga gcaaactatg aacattgtcg atcaacaaac ttttcgcgat
    24121 gcgatgtcct gcatgggcgc ggcggtcaat attatcacca cggacggtcc agccgggcgc
    24181 gccgggttca ccgccagcgc cgtctgcagc gtgaccgata cgccgcccac attactggtg
    24241 tgcctgaatc gtggggcgtc cgtctggccg gtattcaatg aaaatcgaac gctgtgtgta
    24301 aatacactta gcgccgggca ggagccgctt tcaaaccttt ttggcggcaa aacgcccatg
    24361 gaacaccgct ttgccgccgc ccgctggcag actggtgtga ccggatgccc gcaactggaa
    24421 gaggcactgg tttcgtttga ctgccgtatc agccaggtgg tgagcgtcgg cacccacgac
    24481 attctgtttt gtgccataga agcgattcat cgtcacgcca ccccctacgg gctggtgtgg
    24541 ttcgatcgca gttatcacgc gttgatgcgc cctgcttgtt aacctcctta aggagacagc
    24601 tgatggcaat gttcggtttt cctcactggc agttaaaatc gacctctaca gagagcggcg
    24661 tggtcgcgcc ggatgaacga ttgccgtttg ctcagacggc agtaatgggc gttcaacacg
    24721 cggtggcgat gtttggcgcg acggtgttaa tgccgattct gatggggctg gatcccaatc
    24781 tttccatttt gatgtcgggg atcggcacgt tactgttttt ctttattacc ggcgggcgcg
    24841 tccccagtta tctcggttcc agcgcggctt ttgtcggcgt ggtgattgcc gcgacgggct
    24901 ttaacggtca gggcattaac ccgaatatca gcatcgcgct gggcgggatt atcgcctgtg
    24961 ggctggttta taccgtcatt ggcctggtgg tgatgaaaat cggtacgcgc tggattgaac
    25021 ggctgatgcc gccggtggtg acgggtgcgg tagtgatggc gattggcctg aacttagcgc
    25081 ctatcgcagt gaaaagtgta tcggcttcgg cctttgatag ctggatggcg gtaatgacgg
    25141 tgctgtgtat tgggctggta gcggtgttta cccgtggcat gatccagcgg ttattgattc
    25201 tcgtaggatt aattgtcgcc tgtctgctgt atggcgtgat gaccaacgtt ttagggctgg
    25261 gaaaagccgt cgattttact ctcgtcagcc atgctgcctg gttcggtttg ccacattttt
    25321 ccactcctgc ttttaatggt caggcaatga tgctgattgc gcccgtggcg gtgattctgg
    25381 tggcggaaaa cttaggccat ctcaaggcgg tcgccgggat gaccggacgc aatatggatc
    25441 catatatggg gcgggcattc gtgggcgatg ggctggcgac gatgctttcc ggctctgtcg
    25501 gcggtagcgg cgtgaccacc tatgctgaaa atatcggtgt gatggccgtg acaaaagtct
    25561 actcgacgct ggtgtttgtt gctgctgccg tcatcgccat gctgttgggc ttttcgccga
    25621 aatttggcgc gctgattcat accattccag caccggtgat tggtggtgca tcaattgttg
    25681 tgttcgggct gattgccgtc gcaggcgcaa ggatctgggt acaaaaccgt gtcgatttaa
    25741 gccagaacgg taatttgatt atggtcgcag taaccttagt gctgggcgcg ggtgattttg
    25801 ctctcacgct aggcggtttt acgttgggag ggatcggtac agcaaccttt ggcgcgattt
    25861 tactcaatgc gttgttaagc agaaggttgg ttgacgttcc accgccagaa gttgttcatc
    25921 aggagccata aacagaaaac cgccggggta accggcggtg atgacttttt gacgagcttc
    25981 agacaaaaat acattcgtca ttgattagcc cggctagttg tcgcttttgc cgtgactgct
    26041 cttgccacct tttttacctg cttcagatgc gcgctgcggg tcatttttga aattaccccc
    26101 gctgtgctgt ccaccttttt tacctgcttc tgatgctctt tcgcggtctt ctgcaaaatt
    26161 gccggaaccg cctcgatggt ttgccattac tgacctccgt ctgggtatag acatcatatt
    26221 gcatagaatt aacgcggcgt atggcggctg ccatattttc gctacgtggg actaagctta
    26281 gtgaactgac acggtcaggc ccgcatttag taatatcctg caacagcttc ttagcgagaa
    26341 tagatatcat ctccaataat tatccataag ccgctcttat gattaaaaaa acgcgatgag
    26401 ataaaaatgt gtcattttgc gacaaaatta cgcgcttgtg aaagttgtta taaatcaaat
    26461 aagtggttgt gaaatttgca ctctgaaaag gacgtcttat ctttaaaaga gtggtagcga
    26521 atcgctacgg aatagagata acacgaggag tggttagaaa tggctaaagt tctggtgctt
    26581 tattattcca tgtacggaca tatcgaaacg atggcacgcg cagtcgctga gggtgcaagc
    26641 aaagtggatg gcgcagaagt tgtcgttaag cgtgtaccgg aaaccatgcc gccgcaatta
    26701 tttgaaaaag caggcggtaa aacgcaaact gcaccggttg caaccccgca agaactggcc
    26761 gattacgacg ccattatttt tggtacacct acccgctttg gcaacatgtc cggtcaaatg
    26821 cgtaccttcc tcgaccagac gggcggcctg tgggcttccg gcgcactata cggaaaactg
    26881 gcgagcgtct ttagttccac cggtactggc ggcggtcagg aacaaaccat cacatccacc
    26941 tggacgaccc ttgcgcatca cggcatggtg attgtcccca ttggctacgc agcgcaggaa
    27001 ttatttgacg tttcacaggt tcgcggcggt acgccgtacg gcgcaaccac catcgcaggc
    27061 ggtgacggtt cacgccagcc aagccaggaa gaactgtcta ttgctcgtta tcaaggggaa
    27121 tatgtcgcag gtctggcagt taaacttaac ggctaatctt caacaggagg atacgcatgc
    27181 caactcaaga agcgaaagct catcacgtcg gtgaatgggc atctctgcgc aatacgtcgc
    27241 cggaaatagc cgaggccatt ttcgaagtcg ccgggtacga cgaaaaaatg gcggaaaaga
    27301 tttgggaaga aggtagcgat gaagtgttag tcaaagcctt tgctaagacc gataaagatt
    27361 cgcttttttg gggcgaacag accatcgaac gtaaaaacgt ttaatacgcg actcccccgc
    27421 ttctcgcggg ggagttttct gttatttcac cgcttcattc aacacgctat caaacttatc
    27481 cattgggcag aaaccgttgg catctatcgg acaaccgctt aattccagcg tcacgcgctg
    27541 cgcaggtgcc tgaagggtta acgcgtcagc attacgtaac tgttctgcac tctggtacac
    27601 atactcgatt ttcatcaggt cgcgattggc tttgctgtca tgccaacgct ggaaaacgat
    27661 tttgccgcca atcggcgtgc gttcgttctg gtcatgcaac tgatacggtt tgaaatccag
    27721 cgccgttaac agagaggcaa tgttggagtc gtgcccaact aacaccgtga ttttcggtgc
    27781 actggtgcga tcggtgacca gggctttgtc gatataactg accagcggtt tcgcaacatt
    27841 gcgcgccact tccggtgagg taaacaggct gtcctggtaa ccgtttttaa gcttcgacaa
    27901 caccttccac tgctggtcag atttgatttc tccccaggcc acctgatcca tcggaaaacc
    27961 ttcgtagtat tgcaaagtaa acgcatctac cagcgagttg ccgactttca gcggcccgga
    28021 aacacctggt tcttgttgat acttcgcgct aaaggtattt ttgccatcca ccagcgaaca
    28081 ttgttgtttc tctttacagg caggggaatc tttatagtta acgatttttt ccagtagctg
    28141 gtagctgtcg gtaagctgga gtttgctgag ctctttctcc attgccgcca ccgcctgttc
    28201 actgaatgcg gcggaatcat cggtgatcac tgggttaaag gttgggtcca tagtgcccat
    28261 tttttcctga tgatgaacag gaatatcaca cccggggaat gcgccggtaa taaagaactg
    28321 tgcagtcgca acggtacgtt gcagactatt ggcataggca tatacggtgt ccggcggcgg
    28381 gcattccccc gattttacca tcccctgctc tgccagccat tcacgcatgt aatggcccat
    28441 atacacttcg agcacgccac ctttggtggt gagttgccca ccggggacgt cccattctgg
    28501 ccatttattc ggcgtcgact gctccagcac actgccattg ttcgccagcg gcgcacgtaa
    28561 gttatggcgg ctcatcatga gcacttgctg tagctgatag ccttccggta cggtttgtgc
    28621 ctgagcattt gaagcgagta aaactatccc tgccacagtt gcggcgatta gcgttttgtt
    28681 cattcctggc acctcttttg ttatcaatca ctaaagagtg tgacagaaat atgacttttt
    28741 tccgggaaca ggttcgcaaa atggcttgtt gaaaacctgt ttttctatat acatcttcaa
    28801 aaacaggcaa ggtttgacgc aaactatgcc tgattagccc ctgcctcgcg tacgccgctg
    28861 ataattgcac ctgtggctaa acagaatatc tgcggcgttt ccaatacaga gacaatattt
    28921 accaataaaa aagtgtgaga ctaataacaa tggttttagc atatgccttt gccattaaat
    28981 tgcataacga tttaaatagt caagttgcgc attcatttcc ttattgttta acttgtaagc
    29041 ctgagcaaaa agttcaggag ggaggtaaac gtcataatga ttgccggtat ttattaaata
    29101 accatattca agatcttcat ctgttgaaaa ggattcattt cccaaggaga ttgatgccac
    29161 attactatta acaataaata atgagcaaat gctatctgaa agaacagcaa gaaaattctt
    29221 caccgcttta atcctatccg acgtgtcggt atctaaagca agcaaattct tgttatcatt
    29281 aaatataaac tcgggtaaat gcaggataca tttttcgtta ttcaagcaac tatcaatgaa
    29341 ttctttttta aaaaacagaa agctccatga cataagacta cccacaccct gaattttgtt
    29401 gtcagattgc agatcaacgc caaataatgt cttaacatct tccctgagat cacaaaatgc
    29461 agcatcaaca agagcctcga ttgttggttt atcaacaatt ttaataaact cttcgtatac
    29521 ttccttttgc gtcttatgac tgcccagcgc ccatgaataa tcatgttgtt ttaaaaccag
    29581 tatggctctt aacagacaat atccatcgcc atctactgga gcgtatttta attcataagt
    29641 tttattaatt tcactctgaa taaaagatat accagacaaa tcaataccag catctaaatt
    29701 aaatattccc ggcgtattta accgttctgg ctggacactt atattttcat cttctaaaca
    29761 atactcagaa aaaacatcat cacacgcatt aatgttttca atatattcca taaagccata
    29821 ccaataataa cctttaaaat ctgtgataga ctctgaagct ctgcccggca aggacactct
    29881 aacaacatca atatcttgaa catattgtat agtatatgtc atacctcctg aaacccctga
    29941 ataaattttt gtatcattag tacgcaatgt tctcataaat aatgatagat cattatcatt
    30001 ctgcatttcc tcaaattctt tttctagtga ttcgcattcc a
//
LOCUS       DAOTRK010000054        29305 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.054, whole genome shotgun sequence.
ACCESSION   DAOTRK010000054 DAOTRK010000000
VERSION     DAOTRK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.054"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(91..1524)
                     /gene="bglA"
                     /locus_tag="QIE41_002234"
     CDS             complement(91..1524)
                     /gene="bglA"
                     /locus_tag="QIE41_002234"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDS3531116.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            1569..1880
                     /locus_tag="QIE41_002235"
     CDS             1569..1880
                     /locus_tag="QIE41_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDS3531117.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            2044..2703
                     /locus_tag="QIE41_002236"
     CDS             2044..2703
                     /locus_tag="QIE41_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDS3531118.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(2780..3760)
                     /gene="ygfZ"
                     /locus_tag="QIE41_002237"
     CDS             complement(2780..3760)
                     /gene="ygfZ"
                     /locus_tag="QIE41_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDS3531119.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLATVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            4003..4269
                     /gene="sdhE"
                     /locus_tag="QIE41_002238"
     CDS             4003..4269
                     /gene="sdhE"
                     /locus_tag="QIE41_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDS3531120.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            4250..4657
                     /locus_tag="QIE41_002239"
     CDS             4250..4657
                     /locus_tag="QIE41_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDS3531121.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(4697..5218)
                     /gene="fldB"
                     /locus_tag="QIE41_002240"
     CDS             complement(4697..5218)
                     /gene="fldB"
                     /locus_tag="QIE41_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDS3531122.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            5330..6226
                     /gene="xerD"
                     /locus_tag="QIE41_002241"
     CDS             5330..6226
                     /gene="xerD"
                     /locus_tag="QIE41_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDS3531123.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            6251..6961
                     /gene="dsbC"
                     /locus_tag="QIE41_002242"
     CDS             6251..6961
                     /gene="dsbC"
                     /locus_tag="QIE41_002242"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDS3531124.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            6967..8700
                     /gene="recJ"
                     /locus_tag="QIE41_002243"
     CDS             6967..8700
                     /gene="recJ"
                     /locus_tag="QIE41_002243"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDS3531125.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSAAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            8791..9889
                     /gene="prfB"
                     /locus_tag="QIE41_002244"
     CDS             join(8791..8865,8867..9889)
                     /gene="prfB"
                     /locus_tag="QIE41_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDS3531126.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            9899..11416
                     /gene="lysS"
                     /locus_tag="QIE41_002245"
     CDS             9899..11416
                     /gene="lysS"
                     /locus_tag="QIE41_002245"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDS3531127.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(11459..12007)
                     /gene="idi"
                     /locus_tag="QIE41_002246"
     CDS             complement(11459..12007)
                     /gene="idi"
                     /locus_tag="QIE41_002246"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDS3531128.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            12062..12133
                     /locus_tag="QIE41_002247"
     CDS             12062..12133
                     /locus_tag="QIE41_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531129.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(12130..12255)
                     /locus_tag="QIE41_002248"
     CDS             complement(12130..12255)
                     /locus_tag="QIE41_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531130.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(12257..13705)
                     /gene="uacT"
                     /locus_tag="QIE41_002249"
     CDS             complement(12257..13705)
                     /gene="uacT"
                     /locus_tag="QIE41_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDS3531131.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            14141..16060
                     /gene="ygfT"
                     /locus_tag="QIE41_002250"
     CDS             14141..16060
                     /gene="ygfT"
                     /locus_tag="QIE41_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDS3531132.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            16060..16548
                     /locus_tag="QIE41_002251"
     CDS             16060..16548
                     /locus_tag="QIE41_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS3531133.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(16584..17951)
                     /gene="ghxQ"
                     /locus_tag="QIE41_002252"
                     /gene_synonym="ygfQ"
     CDS             complement(16584..17951)
                     /gene="ghxQ"
                     /locus_tag="QIE41_002252"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDS3531134.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(17987..19303)
                     /gene="guaD"
                     /locus_tag="QIE41_002253"
     CDS             complement(17987..19303)
                     /gene="guaD"
                     /locus_tag="QIE41_002253"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDS3531135.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKLLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(19321..20721)
                     /gene="xanQ"
                     /locus_tag="QIE41_002254"
     CDS             complement(19321..20721)
                     /gene="xanQ"
                     /locus_tag="QIE41_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDS3531136.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(20886..23756)
                     /locus_tag="QIE41_002255"
     CDS             complement(20886..23756)
                     /locus_tag="QIE41_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDS3531137.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(23753..24532)
                     /locus_tag="QIE41_002256"
     CDS             complement(23753..24532)
                     /locus_tag="QIE41_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDS3531138.1"
                     /translation="MIEQFFRPDSVEQALELKRRYKDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(24583..25911)
                     /gene="ssnA"
                     /locus_tag="QIE41_002257"
     CDS             complement(24583..25911)
                     /gene="ssnA"
                     /locus_tag="QIE41_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDS3531139.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATDRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(25914..29012)
                     /gene="ygfK"
                     /locus_tag="QIE41_002258"
     CDS             complement(25914..29012)
                     /gene="ygfK"
                     /locus_tag="QIE41_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDS3531140.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQNEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWEEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTTLKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLSDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSITVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         7243 a         7389 c         7639 g         7034 t
ORIGIN      
        1 cggctgaggc gtaatataaa atattgcgag ctcaatatat taaaagagtg ccaggcctga
       61 tgagcggagc acatcaggca catcggctaa ttacagattc tcgccgttgc tggcaatcac
      121 ctctttgtac cagttaaagc tcttcttacg tgaacgcgac atatcgccag taccgtcgtc
      181 atgtttattc acatagataa agccgtagcg tttgctgtac tgcccggtgg tgaacgacac
      241 acagtcgatg cagccccacg gcgtgtagcc catcaggtcc acgccatcgt aagtcaccgc
      301 ttttttcatc tcttcaatat gggcacgcag gtaatcaatg cggtaatcgt cattgatgct
      361 gccatcatct tccactttgt cgtaagcgcc aaaaccgttt tcgacaataa acagcggctt
      421 ctggtaacgc tcatacagct cgcaaagtgc gtagcgcaga ccaaccggat caatctgcca
      481 gccccagtca gacgctttaa catacggatt cggtacgctg ccttcgaaac cagaaatcgc
      541 atcgccggtg ccaccttcgg ccttcaccgc gttggtcatg taatagctga agccgagata
      601 atcgcaggtg ccttcgcgca gtacatccag atcgccgtct tccattttga tgttaaatcc
      661 gcggcgttcc cactcgttca acacatagga cgggtaatag ccacgcaact gcacatcggt
      721 aaagacgtaa cgttcgcgca tcgactcctg ggcaaacatc acatcgtccg ggttacagga
      781 gtaaggatag agcggcacca tcgccagcat acaaccgact ttcatctccg ggttgatgcg
      841 atgcgcggct ttcaccgcca gggcgctggc gacaaactgg tgatgcagca cctgatacat
      901 ggtctcttcc gggttttcat gttcggtata caccacgccg gagcagcagt aaccgaacag
      961 cggtgcacgc cagttacgct ggttgttaat ttcgttgaag gtcatccagt atttgacttt
     1021 gtgcttatag cgttcgaata ccacttccgc gaaacggaca aagaaatcaa ccactttacg
     1081 gttggtccag ctaccgtatt gctgcaccag atgcagcggc atttcaaagt gggagagggt
     1141 gatcaccggt tcgatgttgt atttcagcag ttcatcgatc atatcatcgt agaacttcag
     1201 cccttcttcg tttggctgag cttcatcgcc ttttggaaaa atgcgcgtcc aggcgatgga
     1261 agtacgaaaa catttgaagc ccatttcggc aaatagcttg atgtcctcct tgtagtgacc
     1321 ataaaaatca acggcttcat ggtttggata gtatttgcca ggcacaactt ctttggtgat
     1381 ttcgcgcggc acgccgtgtg cgccaccggt caaaacgtca caaatgctcg gccctttgcc
     1441 gcctttgttc cagccgcctt cgacctgatg agcggcaact gcgccgcccc ataagaaatc
     1501 tttcggtaag gtgagttttt tcactatcat aggctccaga tgagtcaatt cttacaggaa
     1561 atattaccat gcagccaaac gacatcactt tttttcaacg tttccaggat gacattctgg
     1621 ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc gcacttcaaa acgggtgatg
     1681 tgcttcgtgt cggacgtttt gaagatgacg gttatttttg cacgattgaa gtcaccgcaa
     1741 cctcaaccgt aacgctggat acgctgacgg aaaaacatgc agagcaggaa aatatgaccc
     1801 tgactgaact gaaaaaggtc attgccgaca tctatcccga ccagacacag ttttatgtga
     1861 ttgaatttaa atgtctttaa attgaactca cgcctgttag ttgaaatttt gcatttaatt
     1921 tcatgatttt tcggagatta atctcaaatc agaatttatt ttagctaaca ggtgttcact
     1981 ggaactattc tcagttacgc tggagaggta tacatgtgcg tgtacgtttt tccggagtaa
     2041 gttatggttc agaagcccct cattaagcag ggatattcgc tggcagagga aattgccaac
     2101 agcgtcagtc acggcattgg gctggtgttt ggtatcgttg ggctggtgtt gctactggtt
     2161 caggcggtgg atctcaatgc cagcgccacg gcgataacca gttacagcct ctatggcggc
     2221 agtatgatcc tgctgttcct cgcttcgacg ctctatcacg ccattcccca tcaacgggca
     2281 aaaatgtggc tgaagaaatt tgaccactgt gccatttatc tgttgattgc cggaacctac
     2341 acgccgtttt tgctggtggg gctggattct ccgttagcgc gcgggttgat gattgttatc
     2401 tggagcctgg cattgctggg tattctgttt aaactgacca tcgcgcaccg attcaaaatt
     2461 ttatctctgg tgacctatct agcgatgggc tggctgtcgc tggtggtaat ttatgaaatg
     2521 gcagttaagc tcgcggcggg cagcgttacc ttactggcgg taggcggtgt ggtttattcg
     2581 ctcggggtga ttttctacgt ctgcaagcgc attccataca atcatgccat ctggcacggc
     2641 tttgtgctcg gcggtagtgt gtgccacttt ctggcgatct atttgtatat tgggcaggcg
     2701 taattggcgt gcctttgtag gcctgaaaag acacaccagt gtcgcatcag gcgataaatg
     2761 cctgatgcga tacagagatt tactcttcca gcgaatatgg cagtggctcg atacgcaatg
     2821 tattcgcatc gtcacgcacg cggaagatgc tgtccggttc catatcgtta ttcatgacaa
     2881 cctgcaccac aacctgacca tcttccagtt tgaccgtagc cagcaccgta ccggtacgac
     2941 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcgc
     3001 tacccttcag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
     3061 tctcttgtcc ggtatagcag cctttcttaa agctgatacc gcccagcgcc tggaggttgg
     3121 tcgcctgggg gataaactgc ccgctgttgg cggcatcaat caccggaaaa cccgcttcaa
     3181 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
     3241 tcagcatatt ggcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
     3301 accatagcaa cgtggtcgcg ccttctttga ctacctgttt ttctctcgaa ggtagttcac
     3361 taaagagatt tgccaacgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
     3421 cgtcgtctgg cgcgatggtc actttagaga acaccgcata ttttttcagt tcagtcagct
     3481 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
     3541 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
     3601 cagtcatctg gctgacatct gctgtcacct ggccttgcat atatttttcg ctgtccgcgc
     3661 cagtaatagt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagtggc aaacgggcag
     3721 aagccgtagg ctgacgggga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
     3781 aacggcgtat taataactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
     3841 gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga agcccttgaa ttgttgtgaa
     3901 ttaatttcgc gatcgcgatt gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
     3961 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
     4021 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
     4081 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtttg
     4141 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
     4201 gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
     4261 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
     4321 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
     4381 gttactttcg ctggtggtgt ttgattgcgt tcgcagccag cggcgcatta atgctcgcca
     4441 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
     4501 cgtcaaagcg ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgacagcgg
     4561 aaaacggcaa catttatggc tggcagctga cagcatggac gaagccgaat ggcgggattt
     4621 acggcggata ttgttacaac aagagacgca aagataaaga tgacggcaaa gcatatgtgc
     4681 taagccgtca gtgacatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
     4741 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
     4801 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
     4861 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
     4921 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaacaattt
     4981 taccttcgag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcctggattt
     5041 cgccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
     5101 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
     5161 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
     5221 cgtaagcttc tcgattctgc tgtggctttt gcggctagtg tatcagaatc gctttagtca
     5281 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
     5341 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
     5401 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
     5461 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
     5521 gggcggatat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
     5581 gtatctttat cgcgaaaagt ttcgtgaaga tgatcccagt gcgcatctcg cttcaccgaa
     5641 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
     5701 accattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
     5761 taccggactg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
     5821 gggcgtggtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggtgaaga
     5881 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgt tgaatggtgt
     5941 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
     6001 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
     6061 tgtgttgcgt cacgcttttg ccactcactt attaaatcat ggtgcggatt tgcgcgtggt
     6121 gcagatgcta ctgggccaca gcgatctctc caccacgcaa atttatacgc atgtcgctac
     6181 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
     6241 aggaaaattt atgaagaaag gttttatgtt atttaccttg ttggcagcgt tttcaggctt
     6301 tgttcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
     6361 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
     6421 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
     6481 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
     6541 gatgatcgtt tataaagctc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
     6601 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
     6661 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
     6721 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
     6781 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
     6841 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
     6901 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
     6961 attcgcgtga aacaacagat acaacttcgt cgccgagaag tcgatgaaac ggccgacttg
     7021 cccgctgaat tgcctccctt gctgcgccgt ttatatgcca gccggggtgt gcgtagcgcg
     7081 caggaactgg aacgcagtgt taaaggcatg ctgccctggc agcaactgag cggcgtcgaa
     7141 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
     7201 gatttcgacg ccgacggtgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
     7261 ggttgcagca atatcgacta cctggttcca aaccgtttcg aagacggtta cggcttaagc
     7321 ccggaagtag tcgatcaggc tcatgcccgt ggcgcgcagt taattgtcac ggtggataac
     7381 ggtatttcct cccatgcggg ggttgaacac gctcgttcgt tgggcattcc ggttattgtt
     7441 accgatcacc atttgccagg cgacacatta cccgcagcgg aagcgatcat taaccctaac
     7501 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
     7561 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
     7621 attcctaatc tggcagaact gctggatctg gtagcgttgg ggacagtggc ggacgtcgtg
     7681 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
     7741 aagtgccgtc cggggattaa agcgctgctg gaagtggcca atcgcgatcc acaaaaactc
     7801 gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg gcgactggac
     7861 gatatgtccg ttggcgtggc gctgttgttg tgtgacaaca ttggcgaagc gcgtgtgctg
     7921 gcgaatgaac tcgatgcact aaaccaaaca cgtaaagaga tcgaacaggg aatgcaggtt
     7981 gaagcgctca ctctgtgcga gaagctggaa cgtagccgcg acacgctgcc aggtgggctg
     8041 gcaatgtatc accctgaatg gcatcagggc gttgttggca ttctggcatc gcgtatcaaa
     8101 gagcgttttc accgtccggt tatcgccttt gcgcctgcgg gcgacggtac gctgaaaggt
     8161 tccggtcgct ccattcaggg gctgcatatg cgcgatgcgc tggagcggct agacacgctt
     8221 taccccggca tgatgctcaa gtttggcggc catgcgatgg cggcaggatt gtcgctggaa
     8281 gaggataaat tcgaactctt tcagcaacgc tttggcgaac tggttaccga gtggttagat
     8341 ccttctttat tgcaagggga agtggtatca gacggcccat taagtgcggc agagatgacc
     8401 atggaagtgg cgcagctgtt gcgtgatgct ggcccgtggg ggcagatgtt cccggaaccg
     8461 ttgtttgacg gtcatttccg tctgttgcaa cagcggctgg tgggcgagcg tcatttgaag
     8521 gtgatggtcg aaccggtcgg cggcggtcca ctgctggacg ggattgcttt taatgtcgat
     8581 accgccctct ggccggataa cggtgtgcgc gaagtgcaac tggcttacaa gctcgatatc
     8641 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
     8701 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
     8761 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
     8821 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
     8881 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
     8941 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
     9001 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
     9061 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
     9121 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgctacct
     9181 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
     9241 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaattatcg aagagtcgga
     9301 aggtgaagtg gcgggtatta aatccgtaac gatcaaaatc tccggcgatt acgcttacgg
     9361 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aagagcccgt ttgactccgg
     9421 cggtcgtcgc cacacgtcgt tcagctccgc gtttgtttac ccggaagttg atgacgatat
     9481 cgatatcgaa atcaatccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
     9541 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt actcacatcc cgaccgggat
     9601 cgtgactcag tgccagaacg accgttccca gcacaagaac aaagaccagg ccatgaagca
     9661 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
     9721 ggaagacaat aaatccgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
     9781 ctcccgcatt aaagatctgc gtaccggggt agaaacccgc aacacgcagg ccgtgctgga
     9841 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
     9901 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
     9961 gcgtcgtgag aagctggcga acctgcgtga gcaggggatt gccttcccga acgatttccg
    10021 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    10081 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgtc gtattatggg
    10141 taaagcgtct ttcgttaccc tgcaggacgt tggcggtcgc attcagttgt acgttgcccg
    10201 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgatat
    10261 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    10321 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    10381 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    10441 caacaccttt aaagtgcgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    10501 cggctttatg gaagttgaaa cgccgatgat gcaggtgatc cctggcggtg ccgctgcgcg
    10561 tccgtttatc actcaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    10621 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    10681 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    10741 tatggcttac gcagattaca aagatcttat cgagctgacc gaatcgctgt tccgtactct
    10801 ggcacaggat attctcggta agacggaagt gacctacggc gacgtaacgc tggacttcgg
    10861 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaactga
    10921 catggcggat ctggacaact tcgactctgc gaaagcgatt gctgaatcta tcggcatcca
    10981 cgttgagaaa agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    11041 agcacatctg atccagccga ccttcattac tgaatatccg gcagaagttt ctcctctggc
    11101 gcgtcgtaac gacgttaacc cggaaatcac agaccgcttt gagttcttca ttggtgggcg
    11161 tgaaatcggt aacggcttta gcgaactgaa tgacgcggaa gatcaggcgc agcgcttcct
    11221 ggatcaggtt gccgcgaaag atgcaggtga cgacgaagcg atgttctacg acgaagatta
    11281 tgtcaccgca ctggaacatg gcttaccacc gacagccggt ctgggaattg gtatcgaccg
    11341 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    11401 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    11461 atttaagctg ggtaaatgca gataatcgtt ttctggcttc gcgatttgtc gcctgcatca
    11521 ccatccacgg actgaatgcc cacggcgtgg catcaatacc gcgtaataca tctgctaaat
    11581 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    11641 cggcaaatac cggacacact tcattttcca caatgccatt cggatcggtg gcgcgatagc
    11701 gaaagtcagg atagatagat tcaggaggcg taatttctac gccaagctca taacggcaac
    11761 ggcggatcac cgcctcttcg ttgctttctc ccagttgtgg gtgcccacaa accgagttag
    11821 tccacacgcc aggccatgct tttttgctaa gggcgcggcg ggtaactaat aattgccctt
    11881 tggcattaaa cagccaactg gaaaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    11941 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    12001 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat agaagagtga
    12061 aatgattaac caagtgagcg tttatcgaca accgcccgtt ttgagcggat gtcgacaggt
    12121 aaaaaccatt taaaaaagca gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    12181 acaatcagaa atgacaggaa tagagagagt aaaaagcaac agcagactga atatagcgcg
    12241 cattaaaaga ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    12301 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    12361 catgatgctg gtagccatta aagaagacgt tcagcacaac ggcgctaagg gttgcgagca
    12421 taatgccgct atgcagcaac ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    12481 agagcgttgg cgtcatgcca acaccgagac tgatcgccac aatatagagg ttgtaacggt
    12541 tagtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    12601 tcaccagccc cgcgccgccc agcacaaatt gcggaatcga ggccaccagc accgccattt
    12661 tgggcaccat gccgaacagg atcaaaatga taccggaagc aatgcacacc cagcggctat
    12721 gaacgcgcgt cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt
    12781 taaacgttcc gcctatcatt gtccctacac catcgacacg cagcccgcga ataatatcgt
    12841 gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    12901 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    12961 taccaaatga catcggcgtg acaatcgcaa accatgaagc atcatgtagc ccggataaat
    13021 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    13081 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    13141 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccggcggccc
    13201 agtcaatacc cacctgaatg atgctaagcc cgatagaagt aatcaccaca ccggtaacca
    13261 gtggcgggaa taaaggcatc aagcgaccga taagtggcgc taataatgtg gtgataaaac
    13321 ccgcggcgat agtggcaccg aatatcccca gcaggccgat atccgggttc ataccaatag
    13381 ctatcattgg tgttacagcg gcgaaggtca ccgacataat caccggcagg cggatcccca
    13441 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    13501 agctaatgag catcgcaata gcttcttttg ataggccgag tcggtcacca atcataagag
    13561 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    13621 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    13681 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag agtaaaaagc
    13741 aaccgtttat gttcatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    13801 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtggtt aaaccacgaa
    13861 aatgagaaga gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta
    13921 atcattctgg catgtcgcac tctcgcattt aatcgttttt atctggatag cattcttttg
    13981 atcggcaatg ttgaaataat gaaagcggca tagattgagg ggcaataatt tgaactcagt
    14041 tgcaaaatta taattaaaac ggtcatgcta cctaatatat gcccgtttat tgcatcgcga
    14101 aatttgagaa gacataactc attaaatgaa agggatgcaa atgaataagt ttatcgctgc
    14161 tgaagctgcg gaatgtatag gctgccatgc ttgtgaaatt gcctgtgcgg tggcacataa
    14221 tcaagaaaac tggccgctga gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa
    14281 aggccaggct gcgaatccgg tggcctgcca tcactgcaac aatgcccctt gcgttacggc
    14341 ttgtccggtt aatgctctga ctttccagtc cgatagcgta caactggacg agcaaaaatg
    14401 tattggttgt aaaagatgcg caatcgcttg cccctttggc gtcgttgaga tggtcgatac
    14461 gattgcacag aaatgcgacc tttgtaacca gcgcagttcc ggcacgcaag cctgtattga
    14521 cgtctgccca acacaggcgt tacggctgat ggacgataaa gggttgcagc agataaaggt
    14581 ggcccgccag cgcaaaacgg cagcaggaaa agcgtcatca gacgctcagc catctcgcag
    14641 tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg
    14701 gaaaacccac tttggcgaaa tctattgcgg gctggatcca caacaagcga cttatgagag
    14761 tgaccgctgt gtttattgtg ccgaaaaagc taactgcaac tggcattgtc cgctgcataa
    14821 cgctattccg gattacatcc gtctggtaca ggaaggaaag attattgaag cggcagaact
    14881 ttgccaccag accagttcct tacccgaaat ctgcggcagg gtatgtccac aggaccgtct
    14941 ttgtgaaggt gcatgtactt tgaaagatca ctctggcgca gtctctatcg gtaatctgga
    15001 acgctacatc accgatactg cgctggcgat gggctggcgt cccgatgtca gcaaagttgt
    15061 tccccgtagc gaaaaagtgg cggtgattgg cgctggacct gcaggattag ggtgtgctga
    15121 tattctggcg cgcgcgggag ttcaggttga tgtctttgat cgccatccag aaattggcgg
    15181 tatgctgact tttggcattc ctcctttcaa actcgataaa acggtattaa gccagcggcg
    15241 agagatattc accgcaatgg gaatcgattt ccatctcaac tgtgaaattg gccgcgatat
    15301 ctctttcaac gaattaacgg cggaatatga tgctgttttc ctcggcgtgg ggacttacgg
    15361 gatgatgcga gcagatctgc cgcatgaaga tgcgcctggt gtcattcagg cgttaccgtt
    15421 cctgaccgcc catacccgcc agctcatggg attgccggag tctgcagagt atccgctgac
    15481 ggatgtggaa ggtaagcgag ttgtggtatt gggcggtggc gatacgacaa tggattgttt
    15541 gcggacttcc atccgcctta atgctgccag cgtgacctgt gcgtatcgtc gtgatgaagt
    15601 cagtatgccg ggttcgcgca aagaggtggt caatgcccgc gaggaaggtg ttgagtttca
    15661 gttcaatgtt cagccgcaat atatcgcttg tgatgaagat ggacgcttaa cggcggtggg
    15721 cctgattcgt accgctatgg gtgagccggg gccggatggc cgccgtcgtc ctcgtccggt
    15781 tgcgggttca gagtttgaat tgcccgccga tgttctcatt atggcctttg gtttccaggc
    15841 acataccatg ccgtggttgc agggcagcgg aattaaactc gataaatggg gcctgattca
    15901 aaccggcgac gtcgggtatt tacccaccca gacgcatctg aaaaaagtct ttgctggtgg
    15961 tgatgcagtt catggcgcag atctggttgt cactgcaatg gccgcaggaa ggcaggcggc
    16021 gcgcgatatg ttaactctgt ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt
    16081 aatccggctg actgtattgg ctgccgtact tgtgaagtgg cctgtgtggt cgctcaccct
    16141 tcagaacagg agttgaatgc cgatatcttt ttaccccggc tgaaggtaca acggctggat
    16201 agcatcagtg cgccggtgat gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgt
    16261 cccgtggggg cgctgacgat gggcgagcag gtggtgcagg caaattctgc ccgttgtatt
    16321 ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga tcaccattca gtcattgccg
    16381 ggggataccc ggcaacaaat cgtgaaatgc gatctctgtg aacagcggga agaggggcca
    16441 gcctgcgttg aatcttgccc gacacaggcg ctgcagttgc tgactgaaag agaactcagg
    16501 cgagtccgcc agcagcgtat tgttgccagc agtgagaatc ctctgtgaaa gaaatctccg
    16561 cgctgaaagg cgcggagaat ctattagatt gcccaaccac ccgcgtaaaa cgcgaccagt
    16621 gcggcagtaa tgatcaccgt accaatattc agcttttgcc attcgcgcgc aaagacgcga
    16681 cccaccacca gtgttacaaa gcccagcata ataccggtaa cgatattaca agtcagaacg
    16741 atgaacacgg cacacaccag gccagccatt gcgtcaataa aatcgttgaa atccagcttc
    16801 gagacgttgc ttaacatcag caaacccacg tacatcagtg cgggagctgt ggcataaccg
    16861 gggatcagaa acgataacgg tgataaaaac agaatcatca ggaacaacac accaaccacg
    16921 gttgcggtta aaccggtttt ccccccggcg gcggttcctg ccgctgattc gatataaacc
    16981 gccgcgggcg ctgcgcccac caggccggag aatattgaac ttactgagtc actggtcagg
    17041 gctttgccgc cgttgatgat ctggttgtct ttatccaaca aattcgcctg accggcgacg
    17101 gcacggatgg tgccagtggc gtcgaacact gcggtcatca ccaatgccag tacactcgga
    17161 agtacagttg gctggagtgc gcccataata tcgaggctga aaatcagaga cttaccatct
    17221 tcgccagtca ggcttggcat cgccaccaga ccgtggtact tcaccgctgg gtcaaagatt
    17281 aagccgatga tcgaaattgc aataatcacc aacaagatcc cgccgggtac gcggcacttt
    17341 tccagaccga agatgaccgc cagccccagt aagctcatca tcaccgggaa ggaggtaaag
    17401 gcaccgagcg ctactggcaa gccttcaatc gggtttttga taaccattcc cacaccgtta
    17461 gcagcaatca gcagcagaaa caggccgata ccgatacctg taccgtgagc gatccccatc
    17521 ggcaaattac gtaagatcca ggtacgcaca ccggttacgg aaatagcggt gaagatgacg
    17581 cccatcagaa agactgcgcc cagtgcgaca ggaacgctaa tttgttgccc gagtaccaga
    17641 ctgaatgcgg taaacgccgt caaggaaata gcgcaaccaa tcgccattgg caaattggcc
    17701 cataacccca tcagcaacga gccgaagccc gcgaccagac aggtggcaac aaacacagct
    17761 gcgggaggaa aacctgcttt gcccagcatt cccggaacga cgataacgga ataaaccatg
    17821 gccagaaagg tcgttaagcc agccagtact tcctgacgaa cggtactgcc acgagcggta
    17881 attttaaaat aattatccag cgcgccctgt ggctttggtg cgtccggtgt ttgtaggatg
    17941 tctccagaca taatgatgtc ctcacgagga tgttaaagtt ttattattag ttgcgttcgt
    18001 acaccagacg accatcaacg taggtgcggt agatcgaacg gtcatcgccc aacgtcatca
    18061 tcacgaacaa tttgtcgact aaagaaacag agttgtcata gcgcagctgc tgtagcggag
    18121 tggcagtggg ttccatcacc acgaaatcag cctctttgcc aggtaaaaag ttgccaatca
    18181 aatcgtcaag gcccagagat ttcgctccgc cgagcgtggc caggtaaaac gcttcatatg
    18241 ccgagaggcg atagccttgt aattgcaata ccttgtaggc ttcgttcagc gtttgcagca
    18301 tgttaaaagt tgttccggca ccgatatctg ttcccatgcc cactttgact ttcttctgcc
    18361 atgctttttt caagttgaat aagccgctgc cgaggtaaag gttggaggtc ggacagaaag
    18421 caatgctgga tttggtttcg ctgagacgat cccactcttt ttcttcgaga tggacgcagt
    18481 gagcaaagac acaattttta ccggtcaggc cgtactgatg gtaaacatca agataaccat
    18541 catggtcagg ataaagcaat ttcacccagg caatttcatc tttgttttca cagagatggg
    18601 tatgtaccca tgtatccgga tattcttctt tcaggcgttg cgccatcgcc atctgttcag
    18661 gagatgaggt aggggcgaag cgtggcgtaa tcgcatacaa cagacgacca tttttgtgcc
    18721 agcgttcaat cagttctttg ctttggtgat agctgctttc ggcagtgtcg agcagataat
    18781 ccggtgcgtt tcggtccatc atcactttac cggcaatcat acgcatattg atatgactgg
    18841 cggcttcaaa cagcgcatca acggattgtg gatgaacagt gccaaacacc agcgccgtgg
    18901 tggttccgtt acgtaaaagc tgcttgatga agaacgccga catctcgcgg gcgtactcta
    18961 aatcctcata acgacgttca gtagggaagg tgtgtttatt caaccactcc agcaattgtt
    19021 caccataggc ccccaccatt tcactttgcg gataatggat atgtgtatcg acaaagcccg
    19081 gtactatcag tttgccgcga tagtcgcgca cgcgaatggt gtcaggaatt tgatgctttc
    19141 cgtcttccca ttcgccaaac cattccactt ttccctgttt aatgagtaat aaaccatcct
    19201 caataaaccg cagcgcagag gcaatttctt ccgggttatc gaccgtacgg gtgacatcaa
    19261 taaaactgcc tcgtaccgct tttaacgtgt gttctcctga catcattgac tcctttactg
    19321 ttaatccatc tcttccgctg aggtaatacc aggcagaacg ttttcctgtc ggtagccacc
    19381 agggaggata atatttaata aaatcgcagt taacccgcca gcacaaatag ggttttcaac
    19441 taatacataa atagaggctg gcaatatttt aaaaatttcg ggatcgtagg agacgccaag
    19501 ccctaaacct aaagacgtgg cgacaataag tgtttctcgg cgctttaaac cgttggtgat
    19561 gatgatgcga atccctgcga tggcaatcat ggaaaacatc aacgtcattg cgcctcccag
    19621 aactgctgag ggaatggtcg tgaagaagcc gccaatcatc ggaaataagc cgaggataac
    19681 cagcattacc gcgatggttc gcccgacata acgtgaagcg acgccagtca tctgaataac
    19741 cccattattt tgcgcaaacg tggttaatgg taatgaaccg acagcggagg cgataacaga
    19801 aaccagaccg tctgccagca cgccgccttt cagtcgggac tgatactctt ctccctgaat
    19861 gggtcggcgg gaaaccattg ccgtggcggt gatatcgccg acagcttcca gcacgctaag
    19921 caaataaatc gtgccaacca ccaggaactg atggaagctg aaactaaacc cgtatttgaa
    19981 tggatgcggg atggtgatta acggcaaatt gcgcatactg ctgaaatcca ccatacccag
    20041 gcataacgac gcgatataac cgacgcatag cccaatggcg atccctccca tgcgtagcaa
    20101 cggactgcga cagcagttaa agccgatcac cacaatcaaa accaataaac caacgccgag
    20161 atgttcgtaa ttgccgaacg taccgctgct tttggctgca aatcctccac caaaatcgat
    20221 aatgccgact ttaatcaggc ttaagccgat catcagtacc acaataccgc tgacagtagg
    20281 cgtaataacc cgacgtaaat agggcaggat aaaagaagag ccgacaacca gaaatgcgcc
    20341 aacgaaggag acgccgagga gcgacgacat gattaactct tcgtgaaaac cgtcgctttt
    20401 catgctgctg cccagcgcaa tcatgaccgt aacaaatgaa aaattgactg actgaattga
    20461 gagaagacca gaaccgacga tgccgtagcg gtttacttgt aaccaggtac caataccaga
    20521 ggcgatcatc gccatagaaa ccagataagc ggttgtttca gcggaaagct gtaaggccgc
    20581 acccacgatt aacgcggggg ttaccatcgg aacgaaaatt gccaacagat gggtaatggc
    20641 accaacgaga gcctgatgaa agggagggcg atcctccagt tcaaatataa ggtcagaacc
    20701 tgcatggttt atatcagaca tcccttcact ccttttaaag taaatttgat catctgaatt
    20761 agtaaaataa attcccacga atttggggcg caggacgcct tgagaacggc ttatctcatt
    20821 ttcgcagtca ctatttagtc gtcgttcaaa cagagaaaca ggccgccgaa gcggccattt
    20881 attatttata ttttttccag cgcggtgagt attttctccg gtgtgaaatg ccattcgcgt
    20941 aaccagatgc cgcatgcatc gtgaattgcg gtagcaatcg ccggagctgc gccatttaca
    21001 ccgatttccg agatcgattt cgccccgaac gggccgactt tatcgtcgct cggcaccagc
    21061 acagcgcgga aatcacgcgg aatatcgcct attttcggtg cgccgtaact gcgtaaatca
    21121 cgcgttaacg ggtgaccttc ggcgtcataa atgatttctt cgctcatact gtggccgatt
    21181 gcacgaaggg tggcaccgta gatttgtccc aacgctaact ctggattgac cggtgtaccg
    21241 caatccagca aggcgtagaa tttatccagg cggatttcac ccgtacgcgt gttgacggca
    21301 acttcagcaa agtttgcgcc atacgggaag gcgaaatcag gcgtgatata actgccagtt
    21361 cccaccagtg aaccaaagcc tgtgccggtt tcgcctttat gggcaatatc cccgaaacta
    21421 acttcgcctt tcttgccgcg tacgacgccc ggcgttgcta gttggacatc tgccactggc
    21481 tcacccaaca tttgcgcgcc gtggaacaga attttctccc gcagattttc cgctgccaaa
    21541 cgcgccgcgt tacccgagaa gcaagtaccg gacgaggcat atgcgccttt atcaaacaac
    21601 gcatgatcgg tatcaccgga gataacatgc acgtcctgcg gggggcagtg cagcacttct
    21661 gctgccagtt tcgtcactac agtatccaga ccagtaccaa tatccgcacc gccagaatga
    21721 acgataaagg tgccgtccga ttccagtttg atcatacagt tagcctgatc gatatccggg
    21781 atacccgatt tctgcatgat aatggcgacg ccgcgaccga tgtgccagtc accattttgc
    21841 ggtttcggtg aagaccattg gatcatctcg cgaccctgac gcaggatctc ttccagtgcg
    21901 cagctggcgg cggaaggaac ggaggtcggc gctttacctt caccgattgc accgagaatt
    21961 ttcagctctt gcccttcgtg tacccggtta cgttcgataa tttccagttg gtcgatctgt
    22021 aactgttcag ccagttccgc taatgccatg gtgatagcga agttaccttt cggtgcgcca
    22081 taaccctgat aagcaccatt tgggcaaatg ttgctgtagt aggtggtgac ctggaaatcg
    22141 acgttatcgc acggatataa cggcagcgac agcgccggtc cgttacacgg tacggtgagt
    22201 gagtggttgc cgtaagggcc agtgttggcg cggaaatcca tcttcactgc cgtcaggcga
    22261 ccatcttttt tcgctcccag tttgacggtg actttcgcga cgtgacgaga ggtgttagca
    22321 ataaactctt cttcacgggt gtagcggaac agtaccggac gcccggtcac gcaggttgcc
    22381 caggcgcaca cttcttccag caggatgtcc tgtttagacc caaaaccgcc gccaactcgc
    22441 tctttaatga catgaacttt atgttgtttc atgcccacga ggcgcgcgac ctggcggcgt
    22501 aagtgccatg gtacctgggt ggaggcgtgg atcaccagac gatcgccgtc catacgggta
    22561 aagcagatat gtgtttcagt cgggcactgc tgcgcctgcg ttgagttata ggttcgctca
    22621 atgatcacat cggcatcggc aaagcctttg tccatatcgc caatatgacc atgaatactg
    22681 gcggcgatat ttttgcgagg gcgagaaccg atcgggaagt tgatgatcat atgctcgccg
    22741 cgctgggctg catggctgtt atcgtcttcc agagtatctg gcgcaccagc aacatacacc
    22801 accggttcat cgtgcacgac aggcgcatct tccgccattg cttcgtcgat cgacattacc
    22861 ggcttaagca cttcatattc aacgtcgatg agcttcagtg cttcgagcgc aatttcttca
    22921 ctttctgcga cgaccgcagc aacgcgatcg ccgacgtgac gcattttctt gccgaacata
    22981 cggcggtcaa gcggtgacgg ttccggtgcg ctctgaccgc ccggggtata gtagatatcc
    23041 gggcaattca ggtgagtaat aacgtgaaca acgcccggta aggcttcagc tttgctgaca
    23101 tccagatgag taatcagtgc gtgagcgtgt gggctacgta acattttaat gacgcaggcg
    23161 tcagccgtta cgcggtcttc aacatagcag ggtttcgcct gcaccatttt cgcggcatca
    23221 gttttaggat aatgcttgcc aatgacttca aggtcgtcac ggaaagtcgg agcgatatca
    23281 atggtggcct gcggattatt tttacgtgca accgccagtt caatgacctg atagtattgc
    23341 tgccagccag catcgcggct aaacagacct gagagtgcat cgtcgatctc ttcgcgggta
    23401 ggtgcgtcga tgcgatcgag aagatcggtg ataatcagag ctgcagctgg atcgttataa
    23461 ccagactgca ccacgccaac atcaaccatg gcctgttgaa ccagacttaa ctcattccat
    23521 ttgcccagag attctgcagt acgaatatct gccttctcta actgtgcggc gataagcaag
    23581 gacgcgttaa cgatattacc gttaaagata attgcgtcag aaccggcaaa cccaaagcca
    23641 tcatcactgt tgcgtacaga gtgcattccc atgttaaaca acagcttttg cacgttttcg
    23701 cctggattaa cggttagctc ctgaggcgcg ccatttaaag taaagtggat gatcatacgg
    23761 cttcctcccc agcctgttgg cagtcggcat acaggtcggc taccagaact cccgtgatat
    23821 agcgtttata ggccacgctg ccccgcaaat cttcctgcgg gaaaatggcg ttggcgacag
    23881 cctgttcaag tgcattgcct tccagatttt gtttttcgac atcgtgcaga cgcagggctt
    23941 tactggcgac gccatccagc gcaattcgca taccgtcgtg gtctgtcatt gcaacggctg
    24001 ccgtcacgac ggttaaacct gcctgagaac ggctaatttt gcgggtcgca caggtgcgat
    24061 acggatcttt aatgataatt tcggttagca ggcgatcgca tgggcaggcc aggtagtcct
    24121 cgattgacag cgtttcgccg ttgccaaaaa ccagttcagc atccagtgcc agcaggacgg
    24181 gaagcagcac cgactcttcc tgccgggcgg cgatttcacc accaatggtc gactgattac
    24241 gaacatggcg tgagtaaaca aaaccgaggg cttcacgcag tgctgcagga ataaatcgcg
    24301 catcacgcag tggctgcaag cgagacattg caccaatccg cagtgcaccg ttatcccagt
    24361 caacccagtc cagttccaga tcctgtaagg aaatggcaat ctttttatcg gtacgggttg
    24421 gtgtagcgtt gagtttgctg cccccggcga accagacggc ttcatccttg tagcggcgct
    24481 tcagttccag cgcctgttcg acagagtcgg gcctgaaaaa ttgttcaatc atcttctttc
    24541 cttatttaac cctcttccct gcgggaagag ggtatttgtc atttatgcca gcgcatccat
    24601 ccgacgccac atactggcag cggcttttct ggcttgcgca taaatggaat cgcaatcgaa
    24661 gttaaactga cggtcttcat agaccatcac gccattgacc atcacgctat gaacgctgcc
    24721 tgagcccata ccgaaagcga tatgcccggc gatattgtct gcaagcagcg gtgtcggcga
    24781 gttgtaatcg caaatggtta aatcggcttt gtaaccggct tccagaaggc caaatttcgc
    24841 gccaaagttg cggctcatca gttcgttgcc gttagccagt gctttggcaa aactgtcagg
    24901 ccacagcgga ccacccgcat cgcgatgttt aaagaaggca aatttcatct cttcaaacat
    24961 gtccgaacca atgccgtcag ttcccaacgc cagattgcgg atgtcgctaa ggtgatggtt
    25021 gtagccgaca tggttgttca tgtttgaacg ggcgttatgc accaggaacg catcgcgctg
    25081 attgagtagg gcgatgtcat ctttcgacaa gtacagccca tgagcgacca gcgttttgct
    25141 gtcgatgaga tcgaattgcg ccagtcgtgc cagcaggtct ttgccgtacc agtggtgact
    25201 gtaggaaacg tcgtaaaggt cttccgcagc gtgaatatgc aaaccacgat ctgtggcttt
    25261 cacggcttca cgcagcatct ccagaccggc atccggcacg gtaaacggcg cgtgagcgcc
    25321 gatatgtgct tccaccagat acggctcggt tgctgctttc ttcgcctcat caatctgacg
    25381 ggcgaagcgg atgttttctt ctacaccttc ctgcaactct ttgatgccgt tgttacggtc
    25441 agtagtttca aaacaggtca tcgcgcgcag gccaactttt aaaaatgcgt tgcgcaatgt
    25501 ggagagcgac ccgccgatat acgccggaga ggcatggtga tcgataaccg atgtacatcc
    25561 gctcttaatc gcttccaggg aacaaatcag tccgctgtaa tagagcgact cttcatcaag
    25621 ggcgcgatcg agtcgccacc agagattttt cagcgttgag atgaaatccg ggcagggggc
    25681 gatgtttgcc ataattccgc gggacagtcc cgagtaaaaa tggttgtgcg agcagacaat
    25741 tcccggcatc acaatccgac catgcatctc tttgtagctg gcatcggggt agcgttgcgt
    25801 cagggcatcg ccgatagcga caatcacatc attttcgatg gcgatatcaa cgccttcctg
    25861 cactttcgcc gggtgtaact gcactgcggt gacattcttc agaatcaaca tgattacacc
    25921 tccacgcggc ccagcagata atgatgatgc tggtggacat ggctaataat gcggcacata
    25981 tcgttcagct ccggtggtac gttgttgaac tgaccttcgc tgtcgatgtt taacacccag
    26041 ctttggttat tcagacgtac tcgtacccgg caatcttcca caaggaagcc aggattgctg
    26101 ctgttatcaa agtcttgcga caggctgaag acggtgattt tgtctttgta cggtttaccg
    26161 ttccacgggc agaactgagc gcagttgccg cattcgttac agtaagcgtc gaggtgcagc
    26221 gtctggaaac ggttctggaa gcctgggacc gtaatggata cgttggcgcg gttcgggcag
    26281 acatccacac acttgctgca aacgtagtta cattcaaggc agcgagcggc ttcctgcgcg
    26341 acaaacgcat cacgatcgtc actgttcacc agagtgacag agatatcgcc tttacgttga
    26401 tagatttccg ccggattgac gttattccag tatttatcgt tctggtggga acggatattt
    26461 tcccgactca ggatggcatc ggtcgcccga cgcgcggttc cgacagcagc gacaatggag
    26521 gatggtccgc gctgtacgtc gccgatcata aagacgtcac tcagacgcgt ttcgccatta
    26581 tggtcgacgt ctggccagcc gtttttgtcc agcggcacgc ccatcgcatt cagggcttca
    26641 gtatcctgct gttcaccaat ggcggtgatc aggctgtcta catgcagtgt tactgtttcg
    26701 ttggtttcaa ccggacggcg acgacctttc tcatcaggtt cgccaagcga cataacgcgc
    26761 aaggttaagg tgccatcagc atcgaaacgt tccggattat tcaggaaacg gaactccacg
    26821 ccgtcgtgca acgcttcttc atactcttcg cgccatgctg gcatctcttg cagtgaacga
    26881 cggtaaacga ccgttgcttt ttctacgcct ggaacgcgta acgccgcacg agcgcagtcc
    26941 attgcggtgt tacccgcccc gacaacgacc acatgtttgc ccagcttgag tgtggtgccc
    27001 ttgttgtatt cacgcaggaa ggggagtgat ttccagacat tttggttgtc gcccgccagt
    27061 ttcacaccgc tatttttatc ggtaccggtg gcaatcagaa catagtggaa gccctgattt
    27121 tttaactgtt caacggtcaa atcgggtgag caaccgtact caaatttcac gccgtgatcg
    27181 gcaacaaaat cgatatcgtg ctgaattaac tctgcaggaa tacggaactg aggaatgata
    27241 tttttcacca cgccgcccgc attggcttcg cgttcaaaca gcgtaaccgg atggcccgct
    27301 ctggcaagga agtaacctgc ggccagaccc gccggacctg caccaatcac ggcaaccgga
    27361 tggcgtgaac cagaaccggc tggtttgtgc cagcgttgct tatattcctc ccaacctttt
    27421 tccagcgcga cttttttcag ttcgcggata ttcagcgcac tgtcgtaatc caggcgggta
    27481 cagttgtatt ggcactggtg atcgcaaata tgaccggtaa tggcgggcag ggcgttgcgt
    27541 tgatagatga gttccagcgc gtcggcatag cggtgttcgc caagcagacg gatgtattcc
    27601 ggaatatctt gcttgatagc gcaggcagta acacaggggg caacgtagca atcggtcagc
    27661 ggcaggtctt cagccacttc aatacgctct tctggcttcc agtgtttctg ggtgtattcc
    27721 atggttaacg cgtctgctgc cagtctgttc agtcgttcga cgtcaacatg gtcaagtccc
    27781 caggcgtcgg agccttccag ctcgcgcatg caggcactta agcgcagata gccgccaggt
    27841 ttcagcaggt cggttgccat agtaataggg cgaataccag tatcaaaaat atcgcggata
    27901 gtcagctgac tggcaccacc ggaataagaa atgggcagtt tgccgtcaaa ggcgcgagag
    27961 agaactgctg caacgttgat ggagagcggg aacagcgcac ggcctgacat atacatctct
    28021 tcaccaggca gtgcgccttt gttattaatg gtgccgagag tgttagtcag ttttacgcca
    28081 aagccgagtg atttttcttt tgccagtgcc atcaggcgtt ccagcatttc cagcgcttgc
    28141 gtcagcttga ggtcgtgatc aaatgactct tcttttaagc cgatgtaacc gaaaccgcag
    28201 acatcgagga tctcacgaac acgcgcgtac cccagtaagg tcgggttgag tttcacaaag
    28261 gtgttgagcc ctttttcttc cagcatgtag cggcaaatgg cttcgatttc atgcggagga
    28321 cagccgtgca tggtggagag ggtaacccct tgtaccatgc tggtggggat gcgtgccggt
    28381 aaggcttgca agctttcgcg tttttcctgc aatccgtggc gggctaaaaa tgcctcattc
    28441 tggagtagct tattaagtgc atcgcggtat tgagcgaatt tcggatggtc agatgcatcc
    28501 atcatattgt cgatgaactg ttgcatcggc ggttgcttaa taccttcgag gttataaccg
    28561 acgctcatat taaagatgaa cgatttaccg gaatcagaag gctggaacat cgcttcgaga
    28621 aggtgcaggg caaaccaggc tttgaggtat tcatcccagg ctttaagcag ggtaaattcg
    28681 gtagaccatt cggtgttaaa gcactcgtct tcggcatcga tacagggctt ttccagctcc
    28741 agacggtcaa gaatttggac ggtttttagt tcgatgaagc gcccgccagt cagccaggaa
    28801 gtaacaatat tttgcgcgag ctgcgtgtgc gggccagcgg cagggccgac gggagtggca
    28861 caggtttcac cgaagacgct aacagtttta ccttttacgg gtgagtaaaa ctgttgctcg
    28921 ggaataccaa agattgagcg ttgttgttgg tattcatcaa atatgcgcgt caaaagttcc
    28981 tcaaacggaa tgggacgcat aatatccccc ataaccctct ccttagctta tacagatatc
    29041 attgagtgac cggtgttgag aaatccggtg gtttaggggg atagagcaac aatcgcacca
    29101 ctcttgccag gtttggcggg aaatataaaa aaatgtgagg cggctcgaaa cttaggagct
    29161 ttttgtgaat gaactcacag gtgccaaatg agaattaaaa tcacttctga aaattattat
    29221 tgccagccca tttatcactt tttctctttt ctcaaaatca gtgattcctt atcattatga
    29281 taaagagtgc tggagggatt aaata
//
LOCUS       DAOTRK010000055        29173 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.055, whole genome shotgun sequence.
ACCESSION   DAOTRK010000055 DAOTRK010000000
VERSION     DAOTRK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.055"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1140
                     /locus_tag="QIE41_002259"
     CDS             <1..1140
                     /locus_tag="QIE41_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDS3531141.1"
                     /translation="NLNGPSGDKLISFSYPVGGVNNIVFYNPTSATPTNAQIKLYWSD
                     LDTSSSRGFVYCTSKSNSSGMPITVRHNMVDSGKMADGHRLYETNVTGLYYTLMLNRF
                     WSYELRSSSPDIYVGDDGWHTVQFSITGDYIRGQCNTSRYQAVGGLLHDATVEFYTDS
                     SFNPAPNTNVQLKHTGDYLYSFANEGPGVPNVSASKMLKIDFNLTDINIQLPTCFTSI
                     LLGESVTRSTVNLGEYTVGQITSDSATPVNFQISLQNCVRISSIKTKLKSNVVGVNNP
                     LLLGNTLTGTDDAKGAGVLIEGLPNSKNSQNFTLKPNDETSSYNDAEDDPGENNGIYN
                     PDYPNANGRTTTQDLKFQATLKRDGNATVKPGGFKATSTFQVTYE"
     gene            1254..2048
                     /gene="panB"
                     /locus_tag="QIE41_002260"
     CDS             1254..2048
                     /gene="panB"
                     /locus_tag="QIE41_002260"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDS3531142.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATQEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            2060..2911
                     /gene="panC"
                     /locus_tag="QIE41_002261"
     CDS             2060..2911
                     /gene="panC"
                     /locus_tag="QIE41_002261"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDS3531143.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELV"
     gene            complement(3277..4221)
                     /locus_tag="QIE41_002262"
     CDS             complement(3277..4221)
                     /locus_tag="QIE41_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDS3531144.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEGRQEVRQEVRQEVRQEVIQEV
                     TQEVSQEFAQRLLSKGMSREDVAEMANLPLAEIDKLIN"
     gene            4495..4875
                     /gene="panD"
                     /locus_tag="QIE41_002263"
     CDS             4495..4875
                     /gene="panD"
                     /locus_tag="QIE41_002263"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDS3531145.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4879..6108)
                     /locus_tag="QIE41_002264"
     CDS             complement(4879..6108)
                     /locus_tag="QIE41_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDS3531146.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVFNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYVTLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(6172..6612)
                     /locus_tag="QIE41_002265"
     CDS             complement(6172..6612)
                     /locus_tag="QIE41_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDS3531147.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWCGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(6717..7487)
                     /locus_tag="QIE41_002266"
     CDS             complement(6717..7487)
                     /locus_tag="QIE41_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3531148.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(7484..8410)
                     /locus_tag="QIE41_002267"
     CDS             complement(7484..8410)
                     /locus_tag="QIE41_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3531149.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            8519..9181
                     /gene="can"
                     /locus_tag="QIE41_002268"
     CDS             8519..9181
                     /gene="can"
                     /locus_tag="QIE41_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDS3531150.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(9222..9758)
                     /gene="hpt"
                     /locus_tag="QIE41_002269"
     CDS             complement(9222..9758)
                     /gene="hpt"
                     /locus_tag="QIE41_002269"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDS3531151.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            9964..12354
                     /gene="gcd"
                     /locus_tag="QIE41_002270"
     CDS             9964..12354
                     /gene="gcd"
                     /locus_tag="QIE41_002270"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDS3531152.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(12401..13951)
                     /gene="cueO"
                     /locus_tag="QIE41_002271"
     CDS             complement(12401..13951)
                     /gene="cueO"
                     /locus_tag="QIE41_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDS3531153.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNNPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            14117..14464
                     /locus_tag="QIE41_002272"
     CDS             14117..14464
                     /locus_tag="QIE41_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDS3531154.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKTLGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            14570..15436
                     /gene="speE"
                     /locus_tag="QIE41_002273"
     CDS             14570..15436
                     /gene="speE"
                     /locus_tag="QIE41_002273"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDS3531155.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDASVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            15452..16246
                     /gene="speD"
                     /locus_tag="QIE41_002274"
     CDS             15452..16246
                     /gene="speD"
                     /locus_tag="QIE41_002274"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDS3531156.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(16284..16646)
                     /locus_tag="QIE41_002275"
     CDS             complement(16284..16646)
                     /locus_tag="QIE41_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDS3531157.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVED
                     AARTLKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16843..19440)
                     /gene="acnB"
                     /locus_tag="QIE41_002276"
     CDS             complement(16843..19440)
                     /gene="acnB"
                     /locus_tag="QIE41_002276"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDS3531158.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            19795..21648
                     /locus_tag="QIE41_002277"
     CDS             19795..21648
                     /locus_tag="QIE41_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDS3531159.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     LTVSDAAAVTLPATVSAPPVTPAVVKSAFNTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVLTANHVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRDGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSDHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(21719..23143)
                     /gene="lpdA"
                     /locus_tag="QIE41_002278"
     CDS             complement(21719..23143)
                     /gene="lpdA"
                     /locus_tag="QIE41_002278"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDS3531160.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(23468..25360)
                     /gene="aceF"
                     /locus_tag="QIE41_002279"
     CDS             complement(23468..25360)
                     /gene="aceF"
                     /locus_tag="QIE41_002279"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDS3531161.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAGPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAVKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAVTGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(25375..28038)
                     /gene="aceE"
                     /locus_tag="QIE41_002280"
     CDS             complement(25375..28038)
                     /gene="aceE"
                     /locus_tag="QIE41_002280"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDS3531162.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(28199..28963)
                     /gene="pdhR"
                     /locus_tag="QIE41_002281"
     CDS             complement(28199..28963)
                     /gene="pdhR"
                     /locus_tag="QIE41_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDS3531163.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         7063 a         7889 c         7112 g         7109 t
ORIGIN      
        1 aaccttaatg gcccatcagg cgataaacta atatcatttt cctatccggt tggtggagta
       61 aacaatattg tattttataa tccaacatca gccacaccga ccaatgcaca aataaaatta
      121 tattggagtg acctggacac atcaagtagc cgtgggtttg tatattgtac ttcaaaaagt
      181 aattccagcg gtatgccaat tactgtccgg cataacatgg ttgactcagg aaaaatggca
      241 gatgggcaca gattatatga aactaatgta accggtctat attatacgtt aatgctcaat
      301 agattctggt cgtatgaact tcgctcaagc tctccagata tatacgttgg agacgatggc
      361 tggcacactg tacaattttc tattacgggt gattatatac gaggtcaatg taatacttcc
      421 aggtatcaag cagtcggagg tcttttgcac gatgccacgg tggaattcta taccgacagc
      481 agttttaacc ctgctccaaa tactaacgtg caattaaaac atactggtga ttatttgtat
      541 tcattcgcaa atgaagggcc aggggttcca aatgtaagtg catctaagat gctaaaaatt
      601 gattttaatt taacagacat caatatccaa ctgccaacat gctttacgtc tatccttttg
      661 ggtgagagcg taacgcgatc aaccgtaaat ctcggggaat atactgttgg acagatcacc
      721 tcagattctg caacacctgt taattttcag atctcgctgc aaaattgtgt tcgtataagc
      781 agtattaaaa caaaactaaa atctaatgta gtaggagtga ataatcctct gttgcttggt
      841 aatactttga caggaaccga tgatgcaaaa ggtgccggag tacttattga agggttaccc
      901 aacagcaaaa attcacaaaa tttcacgctt aagccaaatg atgagacatc aagctataac
      961 gatgctgaag atgatcctgg agaaaacaat ggaatttata acccagatta tcctaatgcc
     1021 aacggtcgta ctacaaccca ggacctgaaa tttcaggcaa cattaaaacg cgatggtaat
     1081 gcaactgtga aacccggcgg atttaaggct accagtacat tccaggtcac ctacgagtaa
     1141 caggtttaac cgcaccgcca tcatctggcg gtgctgtaat tgataaatca ttgcccccac
     1201 caatgtgact gataaaatgc cagacagcac gacattactt atcaggatac gttatgaaac
     1261 caaccactat tgcttcattg caaaaatgca aacaagaaaa aaaacgtttc gcgaccatca
     1321 ccgcttacga ctatagcttc gcaaaactgt ttgccgatga agggcttaac gtcatgctgg
     1381 ttggcgattc actgggcatg acggttcagg ggcatgactc cactctgcca gttaccgttg
     1441 ccgatatcgc ctaccacact gctgctgtac gtcgcggtgc gccaaactgt ctgctgctgg
     1501 ctgatctgcc gtttatggcg tatgccacac aagaacaagc ctttgaaaac gccgcgactg
     1561 tcatgcgtgc cggtgccaac atggtcaaaa ttgagggcgg tgagtggctg gtagaaaccg
     1621 tacaaatgct gaccgaacgt gccgttccgg tttgtggtca tttaggccta accccacagt
     1681 cagtaaatat tttcggtggc tacaaagtcc aggggcgcgg cgatgaagcg gccgatcgac
     1741 tgctcagcga tgcattagct ttagaagctg ctggggcaca actgctggta ctggagtgcg
     1801 tgccggttga actggcaaaa cgtattaccg aagcactggc gatcccggtt attggcattg
     1861 gcgcaggcaa cgtcactgac gggcaaattc tcgtgatgca cgacgcattc ggcattaccg
     1921 gcggtcatat tcctaaattc gccaaaaatt tcctcgccga aacgggcgac atccgcgcgg
     1981 ctgtgcggca gtatatggct gaagtggagt ccggcgttta tccgggcgaa gaacacagtt
     2041 tccattaagg agtgacgttg tgttaattat cgaaaccctg ccgctgctgc gtcagcaaat
     2101 ccgccgcctg cgtatggaag gtaagcgcgt ggcgctggtg cccaccatgg gtaacctgca
     2161 cgacggccat atgaagctgg tcgacgaagc caaagcccgc gccgatgtgg tcgtcgtcag
     2221 tattttcgtt aacccgatgc agttcgaccg cccggaagat ctggcacgtt atccacggac
     2281 cttgcaagag gactgcgaaa agctgaacaa gcgcaaagtg gatttagttt tcgccccttc
     2341 ggtaaaagag atctacccga acggtacaga aacccacacc tatgttgatg ttcccggtct
     2401 ttctaccatg ctggaaggtg ccagccgtcc gggacatttt cgcggcgttt cgactatcgt
     2461 cagcaagctg ttcaatctgg tccagccgga catcgcctgc ttcggtgaaa aagattttca
     2521 gcaactggcg ctgatccgca aaatggttgc tgatatgggc tttgatattg agattgtcgg
     2581 tgtgccaatt atgcgcgcca aagacggtct ggcgctgagt tcccgtaacg gttatctgac
     2641 ggcggaacaa cgcaaaattg cgcctggtct gtacaaggtt ttaagttcga ttgccgacaa
     2701 attgcaggct ggcgaacggg atctcgatga aattattact attgccgggc aagaactgaa
     2761 tgaaaaaggc ttccgcgccg atgatattca gattcgtgat gccgacacct tgctggaggt
     2821 ctctgaaaac agcaaacgtg cagtgattct ggtagccgcc tggcttggcg atgcccgcct
     2881 gatcgacaac aaaatagtcg agctggtgta atacttaact ggcgctacgg ctgatggcgc
     2941 cagttattca ttgccacgtt atctgtaagc gataattcat aacgcagaca acttagttag
     3001 agttaattag cttatcaacc tctgtaagag ataaagttgc catctctgca acgtcttccc
     3061 gagacattcc tttactcaga agacgctgtg ccagccttac cttccggagt ccctctttat
     3121 atcccttctg aaatcccttc tgaaatccct tctcttcaaa ccactgcgcc aacgtctgcc
     3181 tggtttctct ccgtgtcttt atttccatag gttcccttaa ttatctggtc agttaacaat
     3241 aacaattatc tggctataac tgatgatgca gacaacttag ttaatcagct tatcaatctc
     3301 ggcaaggggt aaatttgcca tctctgcaac gtcttcccga gacattcctt tactcagaag
     3361 acgctgggcg aattcctgac ttacttcctg tgttacttcc tgtattacct cttgtcttac
     3421 ctcttgtctt acctcttgtc ttacctcctg tcttccttcc tgtcttcccc tctcttcaaa
     3481 ccactgcgcc agcgtcatca tagactcccc tcccgtttcc ctgtctctca gcacaccata
     3541 aaacaaatcc gcttgttcag tatgaccgcg ttgcagcata tagttttgca tggcaactaa
     3601 ctgacttccg ctagtgtacc cttcgtcaat cagcgtgacc agttgctcca gcaataacat
     3661 taagtcgcgc tggcgaatat gtttttgcag tagttcgaga atcgcaatcc gccgatgctg
     3721 catgatttcg tcatccggtg tgatggtgat atccaccagc gggaaagggt tgttgtaaac
     3781 gcgtcgcgcc agctccggcg agtaaaacat atcaaaccag cacattgaga gcggataagg
     3841 tgtggcctcg ccctgataaa acagaatcgg caccaccagc ggcagcttat cgtgatccgc
     3901 ctccagatgc cggtgcatgg cagcaataga ataacgcatc atgcgaaagg ccattttctt
     3961 atccggcttg ctttgatgtt caatcaccac atgcaaataa ccgggattac cctgcatttg
     4021 cacggaatag agcacgtcag tgctgtgccc tttcaggctt tcttcaataa aactccccga
     4081 ctcaagatgc agcgtgtcga ggtcacacag ttcgcgtagt tccgctggca aatggatatc
     4141 cagaaagtca cgagccgttt ccgcatgcat taaaaattgt ttaaataccg cgtcatgcgg
     4201 tgtggtactc ggtgcatcca tcttccctgc cttccgtgtt gtcttgcgtg aaggcagtat
     4261 ccagtcaagg cgcgccgtcg tcagccgcct ctttattgtt taccgagcag cgttcagaga
     4321 aatttcaatt ggttacaggc gttacatggc acgcttcggc acggtacgca aactgctgat
     4381 gcaaatgccg tcagatgagt agacactaaa caaaaatcgg gcaatactgc gtgagaattt
     4441 cctcaaagca ggcatcgcct gcttcgttaa cgacagggta gaaaggtaga agttatgatt
     4501 cgcacgatgc tgcagggcaa actccaccgc gtgaaagtga ctcatgcgga cctgcactat
     4561 gaaggttctt gcgccattga ccaggatttt cttgacgcag ccggtattct cgaaaacgaa
     4621 gccattgata tctggaatgt caccaacggc aagcgtttct ccacttatgc catcgcggca
     4681 gaacgcggtt cgagaattat ttccgttaac ggtgcggcgg cccactgcgc cagcgtcggc
     4741 gatattgtca tcatcgccag cttcgttacc atgccagatg aagaagctcg cacctggcgg
     4801 cccaacgtcg cctattttga aggcgacaac gaaatgaaac gtaccgcgaa agccattccg
     4861 gtacaggttg cctgataatc acccctgcgg ctggttactc accagccgcg acattgtctc
     4921 cagcgaatcc gttcttaaga tataaagtcg ttttagtaac aacggattat cccccggttt
     4981 tactttgcct ttcatggttg tcaccgccag gtgaaatccg gcttcttttg ctgccttcac
     5041 ggctttgtca ttaaatccgc caaacggata cgaaagatac cagacatgcg gattaaattg
     5101 cgccagagcg cggcgtgaac gtgcaaaatc aaacaggata ttgtgctcac tacggctcag
     5161 taatatgggt cggcgataac catccacccg atgcaaaaaa tgggtatgtg actggaaatc
     5221 aaatacatcg cgaatttcgt taagttcaga aacgctcata aattgcagcg attttgggtc
     5281 ccacttctgc gggtgacgtt tgatgcgcga ggtaacaata aacgccgtcg ccttcatgcc
     5341 atattgtttc aacacaggat acgcatagcg gctcaccgac tttaggccat catcaaaggt
     5401 aatcaccacc gctcgcgcag ggagattgat cttattcttc acgtaacctt ccagttgcgc
     5461 catgctcagt gtcacgtatc ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg
     5521 taccgaggtg gtcgtcgaag tatggcgaaa acgggtattt tcttcgtcgc gcagaatatg
     5581 gtgataggtt agcaccggca ggccattatc gggttgggca tccagcgcgc tgatataggc
     5641 cagtcgatcg ccaatacgga tctgatacca ggtttgattt aacctgtctt tcagtttatg
     5701 caaaattggg tagcgcaaat tgtccgccag taccccaaac ggcgcactgc ccgcactcgg
     5761 cgcgttgaag accggcgtat ctttccaggt tattaagttc tgattactca gcggcttgtt
     5821 gaggtcaccc aaaccatctt caactttttg tcgcccctga accggctcaa gatgaccttt
     5881 atcgataaac cctttaccaa agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc
     5941 cacggcaatg atttgcccgg cccgaatatt ccccacggtc accatcttgt cgccaatttg
     6001 cgcccagacc gcagcatttt cgatggtttg catataacgg gcaggtaatg cggcactgac
     6061 gcttgcggta aacagcatca gcaggagaat aacagcttgt ttgtacatga aaaaataacc
     6121 gagggtgaaa caacgctttt attgtagcaa aagtgccatc agaatcgcgg gttaatactc
     6181 atacaaatta tgtttgagga cgaaaggcgg atttttttgt tgctggtgcc aaagactact
     6241 cacctccggc gcacccagtt cgacaatacg ctcgcgaaac tctgcgctgg ataacgtttc
     6301 acggtagacc agcatctgct caattaacgg taacgtgaca ccagaaatca cttcgcaacg
     6361 ggagtgtttg tggcttaata atgaagccac gcggtacggt ggcgctccgg ctatatcggt
     6421 taagaagatg acaccctcac cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct
     6481 gagcatatta gaacttaatc cgcaccagaa attcacggcc cggcactgaa gaagtgcccc
     6541 atgttttttc tccagcgcat ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac
     6601 ccaacctaac atcgccttcc tccttagccg aaaatcagtc taccctgacc attgttgata
     6661 aagggtgata gccgtcaaaa ctcctctccc ccaaatccag aggagaggaa atagccttag
     6721 ctacgcaaac cacgtccacg ttggatcagc gaccaacaga tcaaataaaa cgccacaata
     6781 aagaccacca gtacaccgaa ggtagtgacc agcggaacat cattgatacc gaggaagccg
     6841 tagcggaaac cactgatcat ataaacgatt gggttcaggt gcgacagccc ttgccagaac
     6901 ggcggcaaca aagtcagtga gtaaaagacc ccgcccaaat acgtaagtgg cgttaacaca
     6961 aaggttggca ccaggctgat gtcatcgaac gttttggcaa acacgccgtt cagcaaaccc
     7021 gcaagggaga acaacaccgc cgtgagcacc agcgttaaag cgacaaatac ccacgaatgc
     7081 acctgaaacg gcacaaaaaa cagtgaaatt gccgtcacca gaatgccaac aaacagacca
     7141 cgcgccacgc caccgccgac atatccggca ataatgacgt gagtcggaac cggcgctacc
     7201 agcagctctt caatattacg ctggaacttg gcaccaaaaa atgatgacgc gacgttagcg
     7261 taggcgttgg tgatcaccga catcatgatc agccccggta caatgaactg catgtagctg
     7321 aagccatgca tatcgccaat acgcgaacca atcaggttac cgaagataat aaagtaaagg
     7381 gtcatggtga tgaccggtgg caccagcgtc tgcacccaga tacgcataaa gcgatggatc
     7441 tctttcgccc agatgctttt tagtgccacc cagtaaagat gcatcatgcg cgatctcctt
     7501 gtttttcatt aaccagtgaa acaaacagct cttccagacg gttagctttg ttacgcatac
     7561 ttaatacctg aatgccctgc tcacttaact gcgtaaatac gctgttgatc ccctgctcac
     7621 gcagcacttc aacttccagc gtcgcggtat cgaccagtcg atactgatag ccatcgagct
     7681 tcggtaacgg gcttttcggt gcgagatcga gaataaaggt ttccgatttc agctttgcca
     7741 gcagcgcctt catcgaggta ttttccacca gctcaccgtg ttgaataatg ccgatattgc
     7801 ggcacagcat ttctgcttct tccaggtagt gtgtggtgag aatgatggtg gtgcctttgt
     7861 cgtttaaatc cttcaaaaag ccccacattg agcggcgaag ttcaatatcc acgcctgcgg
     7921 taggctcgtc gagaatcagt agtttaggtt catgcattaa cgcacgggca atcattaaac
     7981 ggcgcttcat cccgccagat aacatacgcg cacgttcgtt gcgttttccc catagatcga
     8041 gctgtttaag atacttttcg ctgcggatgt acgcttcttt gcgctccaca ccgtagtacc
     8101 ctgcctgatt caccacaatt tgctgcacgg tttcaaacgg gttgaagtta aattcctgcg
     8161 gcaccagtcc caactgacgt ttagcgttca cgacatcctt ctcgagatcg taaccaaata
     8221 cgctgacccg cccggaggtt ttatttacta gagagctgat aataccgata gtggtcgatt
     8281 tcccggcccc gttcggcccg agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta
     8341 ttccacgaag cgcctgaacg ccgcctggat aggttttttt aagctgttga agttccagtg
     8401 caatggtcat aaatttttac ttaccttacg ttcttacact ttatctgtgg tttaaatcgt
     8461 cccggagttg ccctatatta gccaaacgta attatttggt tacaggtcgt taacctccat
     8521 gaaagacata gatacactca tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga
     8581 ggatcccggg ttttttgaga aactggcaca agcgcaaaaa ccgcgctttc tatggattgg
     8641 atgttccgac agtcgcgttc ctgcagaacg tttaaccggt cttgagccgg gcgaactctt
     8701 tgttcaccgt aatgttgcta acctggtcat tcacactgac ctgaactgcc tttccgtggt
     8761 tcagtatgca gtggatgtac tcgaagttga acacattatt atctgtggcc actacggttg
     8821 tggcggcgta caagccgcag ttgaaaaccc ggaactgggg cttatcaaca actggctgct
     8881 gcatatccgt gatatctggt tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg
     8941 ccgtctggat accttgtgtg aactgaacgt catggaacag gtgtataacc tgggccactc
     9001 caccattatg caatcagcgt ggaaacgcgg gcagaaagtc accattcacg gctgggccta
     9061 cggcattcac gacggcttgc tgcgtgatct ggatgtcaca gcgaccaacc gcgaaactct
     9121 tgagcaacgt taccgtcacg ggatttccaa tctcaagctg aaacacgcca accacaaata
     9181 aaaatgccat gccggatgca acacatccgg caacttcaca cttactcgtc cagcagaatc
     9241 actttgccga tatacggcag atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc
     9301 acaaactcat ccgggatcga gaaaccgatg aattctaccg ggacgttcac ttcacgacgg
     9361 gacggtttat ccagcagcgt acaaatcgcc aacgacttcg gttcgcgcag gcttaagatc
     9421 tcacgcactt tcgacagtgt attacccgag tcgatgatat cttcaacaat cagcacgtcc
     9481 ttgccacgga tatcttcatc cagatctttg aggattttca catcacgggt ggtggacatg
     9541 ccgctaccgt agctggaggc ggtcataaaa tcgacttcat gagatacctg aacttcacga
     9601 cacaggtccg ccataaacat aaatgagcca cgcagcagac ccaccagcac catatcgctg
     9661 ccgctgtctt tgtaacgctc agtaatctga cgacccagtt cggcgatacg cgctttaatc
     9721 tccgcttcgg ggatcattac ttctacagta tgtttcatat ctctaaccat atgatttaaa
     9781 aacaaatcac ttcgcacggg caggttttgg caccgcaaca aagcgcaaga cacgcagtat
     9841 accagtaaaa ctattcattc gcggaatctg ttaataaagc tcgaattgtg atgacgatca
     9901 cacatgttaa aacctataat taattgctac taaaatatta atgaattgaa atggtgtctc
     9961 tttatggcaa ttaacaatac aggctcgcga cgattactcg tcacgctaac agcccttttt
    10021 gcagcgcttt gcgggctgta tctactcatt ggcggaggct ggctggtcgc gattggcggc
    10081 tcctggtact accctatcgc tggccttgtg atgctcggcg tcgcctggat gctgtggcgc
    10141 agtaaacgcg ccgcgctttg gctatacgcc gccctgctgc tcggcaccat gatttggggc
    10201 gtctgggaag ttggtttcga cttctgggcg ctgactccgc gcagcgacat tctggtcttc
    10261 ttcggcatct ggctgatcct gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc
    10321 gcagttgccg ctctggtggt ggcgttgctg attagtggcg gaatcctcac ctgggcggga
    10381 tttaacgatc cgcaggaaat caacggcacc ttaagcgccg atgccacacc tgccgaagcc
    10441 atctcaccgg ttgccgatca ggactggcct gcgtatggtc gcaatcagga aggtcaacgc
    10501 ttttcgccgc tgaaacagat taacgccgat aacgtccata acctgaaaga agcctgggtg
    10561 ttccgtactg gagatgtgaa gcagccgaac gatccgggcg aaatcaccaa tgaagtgacg
    10621 ccgattaaag tgggcgacac cctttacctg tgtaccgctc accagcgcct gtttgcgctc
    10681 gatgccgcca gcggcaaaga gaaatggcat tacgatcctg aactgaaaac caacgagtct
    10741 ttccagcacg taacctgccg tggcgtctct tatcatgaag ccaaagcaga aacggcctcg
    10801 ccagaagtga tggcggattg cccgcgtcgt atcattcttc cggtcaatga tggtcgcctg
    10861 attgcgatta acgctgaaaa cggcaagctg tgcgaaacct tcgctaataa aggcgtgctc
    10921 aatctgcaaa gcaatatgcc agacaccaaa ccgggtctgt atgagccgac ttcgccgccg
    10981 attatcaccg ataaaaccat cgtgatggcc ggttcagtca ccgataactt ctcaacccgc
    11041 gaaacgtctg gcgtgatccg tggttttgat gtcaacaccg gggagctgct gtgggctttt
    11101 gatccgggcg cgaaagatcc gaacgcaatc ccgtctgacg aacacacctt tacctttaac
    11161 tcaccaaact cgtgggcacc agcggcctat gacgcgaagc tggatctggt ctatctgccg
    11221 atgggcgtga ccacgccgga tatctggggc ggtaaccgca caccggaaca ggaacgttat
    11281 gccagctcga ttctggcgct gaatgccaca accgggaaac tggcgtggag ctaccagacc
    11341 gttcaccacg acctgtggga tatggatctt ccggcacagc cgacgctggc ggacatcacc
    11401 gttaacggtc aaaaggttcc ggtcatttat gctccagcga aaaccggcaa catttttgtg
    11461 ctcgatcgtc gtaacggcga actggtggtt ccggccccgg aaaaaccggt tcctcaaggt
    11521 gcagcgaaag gcgattacgt caccccaact caacccttct cggaactgag cttccgtccg
    11581 acgaaagatt tgagcggtgc ggatatgtgg ggagccacca tgtttgacca gctggtgtgc
    11641 cgcgtgatgt tccaccagat gcgctatgaa ggcattttca ccccaccatc tgaacagggt
    11701 actctggtct tccctggtaa cctggggatg ttcgaatggg gcgggatttc cgttgatccg
    11761 aatcgtgaag tggcaattgc caacccaatg gcactgccgt ttgtttcgaa actgatcccg
    11821 cgcggtccgg gtaatccgat ggaacagccg aaagatgcca aaggcacggg tacggaatcc
    11881 ggcattcagc cgcagtacgg tgtaccgtat ggcgtcacgc tcaatccgtt cctctcaccg
    11941 tttggtctgc catgtaaaca gccagcatgg ggctatatct cggcgctgga tctgaaaacc
    12001 aatgaagtgg tgtggaagaa acgtattggt acgccgcagg acagtatgcc gttcccgatg
    12061 cctgtaccgg tgccgttcaa tatgggtatg ccgatgctgg gcgggccaat ctccacagcg
    12121 ggtaacgtac tgtttatcgc cgctacggca gataactacc tgcgcgctta caacatgagc
    12181 aacggtgaaa aactgtggca gggccgttta ccggcgggtg gtcaggctac gccaatgacc
    12241 tatgaagtga atggtaagca gtatgtggtg atctccgcag gcggtcacgg ttcatttggt
    12301 acgaagatgg gcgactatat tgtggcttat gcgctgccgg atgatgtgaa gtaaccataa
    12361 tgcccggaga gatccgggca tatttccgaa tacggtcttt ttataccgta aaccctaaca
    12421 tcatccccgt atcttcatgc tccagcaaat ggcagtgcgc catataagca tgttctttcg
    12481 gtgcatcgtg attaaacttc accagcactt cgctgacatt accttctacc ttaacggtat
    12541 ctttccagcc tgcgcgatga gtcgctggcg gtttgccatt ttctgacaag atacggaact
    12601 gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc cacgccagag ataacccaac
    12661 gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac gcctgaccgt
    12721 tgattttatt ggcatggtgg aaatcgaact tcccgccgtg gttcatatga ttcatattgc
    12781 cgtgccccat atggcccatc atctggctgt gatccatccc ggccatcgcc tgatcgccat
    12841 atttctccat cagcatctgc atccccatca tatcgagcat cgggtccata gagagttgca
    12901 gcttacgtac cgtcagccct tccagcgaag gtaatgctgg caggctactt aatgtgtctg
    12961 gcaaagcacc agaggcacta atggcaatcg gctgaatccg cattaccgga tgcggcttat
    13021 caaaaggcgc aatcgccatt cccatctggc tgaccggcag cgtcaccagg tcaaaggggt
    13081 tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcacc ggcagctcgc
    13141 tcaccttcac cggttcaggt agtagaccac cgtcgctggc aatcacatac agcgggcgat
    13201 tgtcgctggt ggcgaaattg agcgagcggg cgttacagcc attgagcaaa cgcaggcgca
    13261 gccaaccacg cggggcagcg tgttgtgggt agattgcacc gttggtcagc aacgtatcgc
    13321 caaaccagcc cacggcggcg gtcatcacat ccagttgata atcaatctgc ccgtcagcgc
    13381 taaatttctt atcctgaacg atcaccggaa catcatcgat accccactgt tttggcagca
    13441 ttaatttcag gatctcgtca tcttcaatca ccaccagacc agccagcccc atcgccacct
    13501 gtcgcccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca ggttgatcaa
    13561 cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgcggg ccgccgtcga
    13621 cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc agttggttat
    13681 agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc agcagattgc
    13741 cgttatagcc ccaggtagtt gcggttttcc cgccaaaggt ggactggcct gcgccaatag
    13801 ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggatcggt aacgttgggc
    13861 gttccgccgc aaatactgcg cggctccaca gcggtaaagc cgaagccacg cccagcgcga
    13921 cggaatattt taagaaatca cgacgttgca tagttatttc cttattctta agcaggcgaa
    13981 acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc gcagacacaa
    14041 tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta tcttctttaa
    14101 gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca gcctgatggc
    14161 cgtctgcgca aacagttacg cgctcagcga gtctgaagcc gaagatatgg ccgatttaac
    14221 ggcagttttt gtctttctga agaacgattg tggttaccag aacttaccta acgggcaaat
    14281 tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg gatctcagta attacgacac
    14341 cttcgacatg aaaaccctcg gtgaagacag ctaccgcgat ctcagcggca ttggcattcc
    14401 cgtcgctaaa aagtgcaaag ccctggctcg cgattcctta agcctgcttg cctacgtcaa
    14461 ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca atgttagcta
    14521 ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca tggccgaaaa
    14581 aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg tagataacgt
    14641 tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg ctgcatttgg
    14701 ccgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt ttatctatca
    14761 tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg tgctgattat
    14821 cggcggcggc gacggtgcca tgctgcgtga agtgacccga cataaaaacg ttgagtcaat
    14881 cacgatggtg gaaatcgatg cgagtgtcgt gtcgttctgc cgtcagtatc tacccaacca
    14941 taacgctggt agctacgacg atccgcgctt taaactggtg atcgacgatg gcgtcaattt
    15001 cgttaatcaa accaaccaga cttttgatgt cattatctcc gactgcaccg atcctatcgg
    15061 tcccggcgaa agtctgttca cctcagcatt ttatgaaggt tgtaaacgct gcctgaatcc
    15121 tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag aggctatcga
    15181 cagtcatcgc aaactcagcc attacttcag cgacgttggc ttttatcagg cggcgatccc
    15241 gacctattac ggcggcatca tgacctttgc atgggcgaca gataacgatg ccttacgcca
    15301 tctctcaacc gaaattattc aggcgcgttt tctcgcttct ggcctgaaat gccgttatta
    15361 caatccggca atccatacgg cagcttttgc cttacctcag tatctgcaag acgcactggc
    15421 ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc atggctttaa
    15481 taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca aaactgccga
    15541 agaacgcgac ggttatattg cttatatcga tgaactctat aatgccaacc gtctgaccga
    15601 aatcttgtca gaaacctgtt ccattatcgg ggccaatatt cttaacatcg cccgccagga
    15661 ttacgaacca cagggtgcca gcgtcactat tctggtgagt gaagaaccgg ttgacccgaa
    15721 actcatcgac aaaacagaac accccggccc actgccagaa acggtcgttg cccatctcga
    15781 taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg gtttatgtac
    15841 cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga aggcgctgaa
    15901 ttacctgatc caccagcttg agtccgatat cgtaaccatt gattatcgcg tgcgcggttt
    15961 tactcgcgac attaacggta tgaagcactt tatcgaccat gagattaatt cgattcagaa
    16021 ctttatgtct gacgatatga aggcgctgta tgacatggtg gatgtaaacg tctatcagga
    16081 aaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact atatgttcca
    16141 caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg cgctgtggaa
    16201 agaaatgcgc gagatttatt acgggcgcaa tatgcccgct gtttaacggc tctggcggag
    16261 ctcgcgggct ccgccagatt tatttacttt tgctgcacga aattacgata agcagccacc
    16321 acctgcagaa aatcctcaac gccgcatagc gacaggcttt cttcgtcgta gtagttcatc
    16381 ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat cacttcttcg
    16441 ccgtccatcc atagcgtgta ctcatgccct gcccgctgcc aggaacgttc gctacctttg
    16501 agcgtgcgcg cggcgtcttc tacttcatca agcaaggcca ggttttcttt cacctcttcg
    16561 ttaaaccagt gcccgaccac ttcatgcccc atggacatac gcacctttac cactccggta
    16621 atatcacgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa tgcgccatta
    16681 ctgcattgca ccttaggttt aattatcgca gcactatgca agattaaaag gtggaaaaga
    16741 ataaggaata aaaataaacc ccctttcctt gagcagaaag ggggtttaaa gaagtgttac
    16801 ttgtcaggta ttcaggagaa cacctgagct attttcaggc tgttacaccg cagtctggaa
    16861 aatcaccccg tccgcttttt cggtgtactg agaaagctgg ttgaagttca ggtaacggta
    16921 agtatcaacg gcagttttat ccacctgtgc cacgtaggtc tggtactctt ccggcgtcgg
    16981 cagtttgcca atcagcgccg caacagcagc cagttccgca gaagccagga agacattcgc
    17041 gccagtaccc aaacggttcg ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc
    17101 tgctacacgc gcctggttac ccatacacag ggaacagcca gggatctcga tacgcgcacc
    17161 actcttaccg aagacgctgt agtagccttc ttccgtcagt tgcgcggcgt ccatacgggt
    17221 tggcggtgcc acccacaggc gggtcggcaa ctggccttta tgcgcatcca gcagtttacc
    17281 cgcagcacgg aagtgaccga tgttggtcat gcaggaaccg ataaacactt cgtcgatctt
    17341 ctcaccctgt accgcagaca gcggacgtgc gtcatccgga tcgttcggag cacacaggat
    17401 tggctcttta atatccgcca gatcgatgtc gatcactgcc gcgtattccg catctgcatc
    17461 ggcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac ggcgttccag
    17521 ggtacgacga tcgccgtaac cttcggcaat catccacttc agcaggacga tgttagagtt
    17581 caggtattca atgatcggtt ctttgttcag cttaatggta caaccggcag cagaacgctc
    17641 ggcggacgca tcggtcagct caaaggcctg ctcaactttc agatccggca gaccttcaat
    17701 ttccagaatg cgacctgaga agatgttttt cttgcctttc ttctcaacgg tcagcagacc
    17761 ttgtttgatc gcatacagcg gaatagcgtg taccagatcg cgcagggtga tgcccggctg
    17821 cattttgcct ttgaagcgca ccagaacgga ttccggcata tccagcggca tgacgcctgt
    17881 tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga tcgggaaacg
    17941 ggtatgggag tcaccaccgg taccgacggt atccggcagc agcatacggt tcagccagga
    18001 gtggattacg ccgtcacccg gacgcagcga cacaccacca cggttcataa tgaagtccgg
    18061 cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga
    18121 ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt catcacgggt
    18181 catcgggccg gtggtgtcct gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc
    18241 cggacgaatg cctttcacgc cgcaggcacg acctaccatt ttctgcgcca gcgagaagcc
    18301 gcggtcgctc tcagcgacat ctttcgcctg acggaacaca tcgctgtgcg gcagaccaag
    18361 tgcttcacgc gctttggtgg tcaggccacg cccgataatc agcggaatac gaccaccggc
    18421 acgcacttca tcaatcagca cgtcggtttt cagctcgaag gtcgccagca gttcgccggt
    18481 ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca attacgtcac ccatgttcag
    18541 gttagagacg tccacttcaa ttggcagtgc acccgcatct tccatcgtgt taaagaagat
    18601 gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca catgtggaat
    18661 atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg aacccgtacc
    18721 cacaacgtca ccgacgtacg ccagcggaaa acctttctgt tgcagagctt caatctgttt
    18781 gatcggacca acaacacctg gctggtctgg ctcaatacct tcacgggcgt ttttcagcat
    18841 cgccagcgcg tgcagtggga tatccgggcg tgaccacgca tccggtgccg gagagaggtc
    18901 atcggtgttg gtttcgccag tgactttaaa gacggtaacg gtcagttttt cagccagcgc
    18961 cgggcgattc aggaaccatt cggcatccgc ccaggactgc attacctgct tagcatattc
    19021 attgcctgct ttggctttct cttctacgtc atagaagtta tcgaacatca gcagtgtgtg
    19081 agaaagtgct ttggcagcaa tcggtgccag tttggcatca tccagcgcgt cgatcagcgg
    19141 atgaatgttg taaccaccct gcatggtgcc cagcagttcg atagcttttt ccggagtcag
    19201 cagaggggat ttggcttcgc ctttagcaat agcagccagg aagcctgctt ttacataggc
    19261 ggcttcatcg acgcctgggg gaacacggtt ggttaacaga tctaacagga attcttcttc
    19321 gcccgcgggc gggtttttca gcagctctac aagcgcggcc atttggtttg catccagggg
    19381 tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt cttctagcac
    19441 gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca cgctcctgtg
    19501 agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc aggattttga
    19561 cgatgtgtta atccgtttac aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg
    19621 gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat aatcaccttt
    19681 ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac tcggattctc
    19741 agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg aattatgaaa
    19801 atgactttgc cgtttaaacc ccatgtgctg gcgctaattt gcagtgccgg gctttgtgcc
    19861 gcctctgccg ggctatatat aaaaagccgc acagtggaag cgcctgtgga agcgcaatcg
    19921 acacaactga ctgtgtctga cgctgccgcg gttacgcttc ctgcaacggt ttccgcgcct
    19981 cccgtaacac ccgccgtcgt caaatccgca ttcaacactg cacaaataga tcaatgggtt
    20041 gcgcctgtcg cgctgtaccc cgactctctg ctttcacaag tgttaatggc atcaacctat
    20101 ccgacaaacg ttgcgcaagc agtgcaatgg tcgcacgata atccacttaa acaaggcgat
    20161 gctgctattc aggcggtatc tgaccagccg tgggatgcaa gcgtcaaatc actggtagcc
    20221 tttccacagt tgatggcgtt gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat
    20281 gcttttctgg cgcagccgca ggacgtgatg gactcggtac agcgattgcg gcaactggcg
    20341 caacaaaccg gctcgctgaa gtcatcaacc gaacagaaag ttattaccac aacgaagaaa
    20401 actgtaccgg taacacagac agtcacggct cccgtcatac catccaatac cgttttaact
    20461 gccaaccacg tcattacaga gccggcaaca accgtcattt ctattgagcc cgccaatcct
    20521 gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg ggccaatact
    20581 gcgtatccgc cggtttatct gccaccacca gccggagaac cgtttgttga cagctttgtg
    20641 cgcggattcg gctatagcat gggcgttgct accacgtacg cgctattcag cagcatcgac
    20701 tgggatgacg acgatcatga ccatcatcat catgacgatg atgattatca tcaccacgat
    20761 ggcggtcatc gtgacggtaa tgactggcaa cacaacggcg acaacatcaa tatcgacgtc
    20821 aataatttca accgtatcac cggtgagcat cttactgata agaatatggc atggcggcac
    20881 aatccaaact accgtgatgg tgtgccctat catgatcagg atatggcaaa gcggtttcat
    20941 caaaccgatg tcaacggcgg catgagcgcc acgcagttac ctgctccaac acgcgacagc
    21001 cagcgtcagg cggcagcaag tcagtttcag caacgaacac acgccgcccc cgtcattacg
    21061 cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaca ctatgggagc
    21121 tatgacgact tccgcgactt cagccgtcgc caaccactga cccagcaaca aaaggacgcc
    21181 gctcgtcagc gttatcagtc ggcctctcct gagcagcgcc aggcagtccg cgagaaaatg
    21241 cagactaacc cgcagatcca gcagcgaaga gaggcagcgc gtgagcgtat tcagtccgcc
    21301 tctcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccgca gaaccagcag
    21361 cgaagagagg cagcgcgtga gcgtattcag tctgcaactc ccgaacagcg ccaggtgttt
    21421 aaggaaaaag tacagcagcg cccactgaac caacagcaac gtgataacgc ccgccagcgt
    21481 gttcaatcag catcacctga gcaacgtcag gtttttcggg agaaagttca ggagagccgc
    21541 ccacaacgtc taaacgacag tgaccatact gtcaggctga ataacgagca acggtcagca
    21601 gtacgcgaac gtctctctga gcgcggagca aggcgactgg aaaggtaaat tacagacgta
    21661 aaaaaagcgg cgtggttagc cgctttttta attgccatat gtcccggcaa acgaagaatt
    21721 acttcttctt cgctttcggg ttcggcaggt cggtaatgct accttcgaac acttctgccg
    21781 ccaggcccac agactcgtgc agagtcgggt gcgcatggat ggtcagtgcg atgtcttcag
    21841 catcacaacc catttcgatt gccaggccga tttcacccag cagctcgccg ccgttagtac
    21901 cgacaatcgc accaccgatc acacggtgag attctttgtc gaaaatcagc ttggtcatac
    21961 cgtctgcgca gtcggaagcg atcgcacgac cagaagcagc ccacgggaag gtggcagttt
    22021 catagctgat gcctttctct ttcgcttctt tctcggtcag acccacccat gcaacttctg
    22081 gttcggtata ggcgatggac gggataactt tcggatcgaa gtagtgtttc ttaccggcga
    22141 taacttcagc ggcgacgtga ccttcgtgaa cacctttgtg tgccagcatc ggctgaccga
    22201 cgatatcgcc gatagcaaag atgtgcggta cgttggtacg cagctgtttg tcaacgcgga
    22261 tgaaaccacg gtcgtccact tccacgccag ctttgcctgc gtcgaggttt ttaccgttcg
    22321 gcacacgacc aatcgctacc agcacggcgt cgtaacgctg cggttcagcg ggtgcttttt
    22381 tgccttccat cgtcacataa atgccgtctt ctttcgcttc aacggcggta actttggttt
    22441 ccagcatcag gttgaatttc ttgctgatac gcttggtgaa gactttaacg atgtctttgt
    22501 cagctgccgg gataacctgg tcgaacattt caaccacatc aatctgtgaa cccagcgcgt
    22561 ggtatacggt ccccatttcc agaccgatga taccgccacc cattaccagc aggcgttctg
    22621 gtacttcttt cagttccagc gcgtcagtgg agtcccagat acgcggatct tcatgcggaa
    22681 taaacggcag ttggatcggg cgagaacccg ctgcaatgat cgcgttgtcg aagttgatca
    22741 cggttttgcc gttctcacct tcaacttcca gggtgttagc cccggtgaat ttacccagac
    22801 cgttgaccac tttgactttg cggccttttg ccataccagc cagaccaccg gtcagctgat
    22861 tgatcacttt ctctttccag gtacgaatct tgtcgatatc ggttttcggt tcgccgaaga
    22921 cgataccgtg ttcagccagc gctttggctt cttcgatgac ttttgctacg tgcagcagtg
    22981 ctttagaagg gatacagccg acgttcaggc aaacaccgcc aagggtgttg taacgttcta
    23041 cgattacggt ttccagacct aaatcagcgc aacggaaggc agcggagtaa cctgcggggc
    23101 ctgccccaag taccacgacc tgagttttga tttcagtact catcatgacc tctatatatt
    23161 tatctccggc ggtcataccc gtcgtctttc aggccgcagc ggcgttagct tcacgccctc
    23221 accccagtca cttacttatg taagctcctg gggattctgg cgcttgctac cttgctgcac
    23281 ccagaaatcc atagggtatg tgaagtaata ccctaaccac caccgggtcg ttctatccgt
    23341 cggtatttta caaaattgtt aacaattttt aaacaacaaa cggcaaccga tttgtctatt
    23401 cgctaataac ctcaatacat tcatgagatt accagaaaaa agccggccgt tgggccggct
    23461 cttttactta catcaccaga cggcgaatgt cagacagcgt gttgttaatg atggtaatga
    23521 aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa ggagagagaa atcggcagca
    23581 tcagacgcgg cacgaactct ttaccattcc acaccggctc catcgcggac ttggaaacgc
    23641 cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa gtgggtagta cccaggccgc
    23701 cgatgctgga gatggtgaag caaccgccct gcatttcgcc cgcagtcagc ttaccgtcac
    23761 gcgctttctt agaaatagtc atcagctcgc gagacagctc gatgatgcct ttcttgttga
    23821 cgtctttgaa taccggaaca accagaccgt tcggggtatc caccgccaca ccgatgttga
    23881 tgtatttctt cagggtcaga cgctgaccgt cttccgacag cgaactgttg aagcgaggca
    23941 tctgctcaag agctgcagca acggctttca tgatgaagac aactggggtt atcttcacat
    24001 ccagcttacg tttcgccgct tcttcgttct gctgtttacg gaacgcttcc agctcggtga
    24061 tatcggtttt gtcgaagtgg gtaacatgcg ggatcatcac ccagttacgg ctcaggttcg
    24121 caccagaaat tttctggata cggcccagtt ccacttcttc gatttcacca aacttgctga
    24181 agtccacttt cggccacggc agcatgcctg ggataccgcc gccagtcacc gccggagccg
    24241 cttctgcacg tttgatagct tctttcacgt aagcctgaac gtcttcgcgc aggatacgac
    24301 ctttacggcc agtgcccttc actttcgcca ggttaacgcc aaactcgcgt gccagacggc
    24361 ggatcagcgg agtcgcgtga acgtaagcgt cgttttcagc aaattcagat ttgccttccg
    24421 ctttcacagc cggtgctgct gccggggctt cagcttttgc tgccggagcc ggcgctgccg
    24481 cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc aacttcgaag atcataatca
    24541 gcgagccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa
    24601 acggagccgg gacttccata gaagctttat cgccttctac ggtgatcagt gactgttcag
    24661 cggcaacttt gtcgcccact ttcaccatca cttcggtcac ttcaacttcg tcaccgccga
    24721 tatccggaac gttaacttct ttcacgccag ctgctgatgc aggggccgct gccggagctg
    24781 cttcctgttt agccgccgga cctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    24841 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgcctg
    24901 caaacggagc aggaacttcc atagaagcct tatcgccttc tacggtgatc agcgactgtt
    24961 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    25021 cgatatccgg aacgttaacg tcttttgccg ccgcagccgc tggtgctgct gccggagctg
    25081 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    25141 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    25201 cgatacccgc ctgcggagat ggtacttcca tagaggcttt gtcgccttct acggtgatca
    25261 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    25321 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttaagc
    25381 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    25441 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    25501 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    25561 agtacccagt acgcggtagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    25621 tttcatatag tcggtagatg ccactgccgg agcgtcgttc atcacctgag cgatgtatgg
    25681 aacgcgcgga gtttccagcg ggtgcagcat gttccagcgt tcacaatcct gaccatcacg
    25741 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    25801 caggatctct gccgcttcac ggacgtgacg caggatagaa ccggagccca gcagctgaac
    25861 tttacctttg ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc
    25921 agcaccttcc ggcattgccg gcatgtggta gttttcgttc agcgtagtga tgtagtagta
    25981 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcatga tgacagcaac
    26041 ttcgtaagcg taagccgggt cgtaagagat acagttcggg atagtcagcg actgaatgtg
    26101 gctgtgacca tcttcgtgct gcagaccttc gccgttcagg gtggtacgac cggaagtacc
    26161 gccgatcagg aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccgatacg
    26221 ctggaagccg aacatcgagt aatagatgta gaacggaatc atcggcagat tgttggtgct
    26281 gtaagaggtc gccgctgcca gccaggaaca ccctgcgccc agctcgttga tcccttcctg
    26341 cagaatctga cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    26401 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    26461 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    26521 catcacgttc agggcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc
    26581 cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    26641 acggcttggc agataaccgt gcagtttctg acgctgagcg tgcagatagg tatgctcttc
    26701 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    26761 attgaaacgg tcgcggatat gacgcacgcc gtccatgttc attttcttaa cctggtgcgc
    26821 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggtat gagcaaggat
    26881 taccgtcgct ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    26941 atcgtgaccg ccacggttca gcgcccagat ctgctcgtca gtccagtctg caaccagagc
    27001 tgcggtttcc ggatatttac cgaagaagtg ttcacgaacg tacgcaccat ctttcgattt
    27061 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    27121 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    27181 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    27241 gtcaagacgc tgcaggttac agttgataac gaagaccagg ttatccagtt tttcacgggt
    27301 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaaggc
    27361 gtaaacggtc tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    27421 agcctggtaa atagcaccaa tcggacccag acccatagat acggtcggga actgccagaa
    27481 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    27541 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacgag cgtaaacgcc
    27601 cggggagatg tggccctgga agtaaaccag atcgccgcca tcctgctcgt tgcgtgcacg
    27661 gaagaagtga ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatgtg
    27721 gccgcccagt tcgaggtctt ttttcgacgc acgcagaacc gtcatgatgg cgttccagcg
    27781 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    27841 aacggggatg gtgttgatgt agttgctgat accggtgcct gcggctacgt tgacaccgcc
    27901 tttgcgggct tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc
    27961 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    28021 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    28081 cctgtcccat tgaactctcg ccggaaagct caataagaca ggttctacgt ttagttgccg
    28141 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag aaaaatcact
    28201 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacggcttc
    28261 tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt
    28321 ccggcttacc ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    28381 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    28441 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttca gtgacagcaa
    28501 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagct
    28561 ctatggcgtg atggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    28621 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgatt
    28681 caggatgatc ggagagcagc tctaccagcg gatcgctgaa gctttgccat aggctgctct
    28741 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    28801 tcgcctcacg caaggaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg
    28861 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    28921 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    28981 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    29041 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    29101 cttgacatat ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacgtaggaa
    29161 agggattttt taa
//
LOCUS       DAOTRK010000056        28257 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.056, whole genome shotgun sequence.
ACCESSION   DAOTRK010000056 DAOTRK010000000
VERSION     DAOTRK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.056"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..891)
                     /locus_tag="QIE41_002282"
                     /pseudo
     CDS             complement(1..891)
                     /locus_tag="QIE41_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1557..2426)
                     /gene="sucD"
                     /locus_tag="QIE41_002283"
     CDS             complement(1557..2426)
                     /gene="sucD"
                     /locus_tag="QIE41_002283"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDS3531164.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEVLKTVLK"
     gene            complement(2426..3592)
                     /gene="sucC"
                     /locus_tag="QIE41_002284"
     CDS             complement(2426..3592)
                     /gene="sucC"
                     /locus_tag="QIE41_002284"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDS3531165.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(3686..4903)
                     /gene="odhB"
                     /locus_tag="QIE41_002285"
     CDS             complement(3686..4903)
                     /gene="odhB"
                     /locus_tag="QIE41_002285"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDS3531166.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSTGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(4918..7719)
                     /gene="sucA"
                     /locus_tag="QIE41_002286"
     CDS             complement(4918..7719)
                     /gene="sucA"
                     /locus_tag="QIE41_002286"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDS3531167.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(8069..8785)
                     /gene="sdhB"
                     /locus_tag="QIE41_002287"
     CDS             complement(8069..8785)
                     /gene="sdhB"
                     /locus_tag="QIE41_002287"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDS3531168.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(8801..10567)
                     /gene="sdhA"
                     /locus_tag="QIE41_002288"
     CDS             complement(8801..10567)
                     /gene="sdhA"
                     /locus_tag="QIE41_002288"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDS3531169.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNADGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(10567..10914)
                     /gene="sdhD"
                     /locus_tag="QIE41_002289"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(10567..10914)
                     /gene="sdhD"
                     /locus_tag="QIE41_002289"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDS3531170.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(10908..11312)
                     /gene="sdhC"
                     /locus_tag="QIE41_002290"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(10908..11312)
                     /gene="sdhC"
                     /locus_tag="QIE41_002290"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDS3531171.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            11693..11800
                     /locus_tag="QIE41_002291"
     CDS             11693..11800
                     /locus_tag="QIE41_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531172.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGKQV"
     gene            12006..13289
                     /gene="gltA"
                     /locus_tag="QIE41_002292"
     CDS             12006..13289
                     /gene="gltA"
                     /locus_tag="QIE41_002292"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDS3531173.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            13679..14245
                     /locus_tag="QIE41_002293"
     CDS             13679..14245
                     /locus_tag="QIE41_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDS3531174.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            14296..16755
                     /locus_tag="QIE41_002294"
     CDS             14296..16755
                     /locus_tag="QIE41_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDS3531175.1"
                     /translation="MDTVNIYRLSFISCLVMAIPSALAVEFNLNVLDKSMRDRIDISL
                     LKEKGGIAPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVLPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDN
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDNDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSNHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVADNVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQLPPLGADIRQEDSGISVGIVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSIHLPEHMEDTANRLILPCH"
     gene            16770..17501
                     /locus_tag="QIE41_002295"
     CDS             16770..17501
                     /locus_tag="QIE41_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDS3531176.1"
                     /translation="MTFMKGLPLLLLVASLCSYAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTAKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQA
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGSYSGRQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            17498..18559
                     /locus_tag="QIE41_002296"
     CDS             17498..18559
                     /locus_tag="QIE41_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3531177.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGSSGGSVEKSEVIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            18711..19757
                     /locus_tag="QIE41_002297"
     CDS             18711..19757
                     /locus_tag="QIE41_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDS3531178.1"
                     /translation="MPILQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLTILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(19754..20545)
                     /gene="nei"
                     /locus_tag="QIE41_002298"
     CDS             complement(19754..20545)
                     /gene="nei"
                     /locus_tag="QIE41_002298"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDS3531179.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVETGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLSAARLDALAHALLDIPRLSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(20581..21315)
                     /gene="pxpA"
                     /locus_tag="QIE41_002299"
     CDS             complement(20581..21315)
                     /gene="pxpA"
                     /locus_tag="QIE41_002299"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDS3531180.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRRAMQLPPETVYAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALEQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(21305..22237)
                     /gene="pxpC"
                     /locus_tag="QIE41_002300"
     CDS             complement(21305..22237)
                     /gene="pxpC"
                     /locus_tag="QIE41_002300"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDS3531181.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(22231..22887)
                     /gene="pxpB"
                     /locus_tag="QIE41_002301"
     CDS             complement(22231..22887)
                     /gene="pxpB"
                     /locus_tag="QIE41_002301"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDS3531182.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(22910..23653)
                     /gene="ybgI"
                     /locus_tag="QIE41_002302"
     CDS             complement(22910..23653)
                     /gene="ybgI"
                     /locus_tag="QIE41_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDS3531183.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGISVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            23924..25405
                     /gene="dtpD"
                     /locus_tag="QIE41_002303"
     CDS             23924..25405
                     /gene="dtpD"
                     /locus_tag="QIE41_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDS3531184.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDTSGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(25448..26866)
                     /gene="phrB"
                     /locus_tag="QIE41_002304"
     CDS             complement(25448..26866)
                     /gene="phrB"
                     /locus_tag="QIE41_002304"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDS3531185.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLRIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGVPECVAAPKVRSSGSIDPALSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPTYLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEEFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(26863..27372)
                     /locus_tag="QIE41_002305"
     CDS             complement(26863..27372)
                     /locus_tag="QIE41_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDS3531186.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMAFYPDAWLSEQRYFELWPRV
                     INLRHAGVL"
     gene            complement(27514..28257)
                     /locus_tag="QIE41_002306"
                     /pseudo
     CDS             complement(27514..28257)
                     /locus_tag="QIE41_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         6819 a         7526 c         6899 g         7013 t
ORIGIN      
        1 acaaccgatt tcgccagcaa ccattcacga aattgctgca agtgcggcga gtcagacttc
       61 tcttcctgcc acgtcataat aaaacgatta ccggtacgaa taggcacatc acagggaatc
      121 accatatcgc cactatccaa atcatgttga atagcgaacc gtggtaacag cgccacgccg
      181 agattagagc gcacggcggc aatcagcatt gaaagcaaat cgaaacgtgg tccgttgttg
      241 accagcggac tgctaacgcc agataaggca aaccactctt cccagccagt aatacgggta
      301 ctttgatgca gaagtggtaa ctctgtaagc agttctgcca ctgacagttt ttgatcggac
      361 attgcaagca aactgccgct gcataccggc aaaatctcct catcaaagat gatattctac
      421 ttccgcccag ggcgcgcaaa aattttcgcg cataatgaca gcatcgtact cccgattaag
      481 aaaatcgccg ttattagcta aagagtgaat attaacaata atatcgggat gtaactttgt
      541 gaattcatgt aaatttggga ttaaccagtg ggtactaaac gtaggattca cggcaagttc
      601 aataacctta actgtaggtt gccaggtcat aatggtattg gtatcgcgtt ccagcttatt
      661 taatgtttct tttactatcc ccaggtaata cttccctgca tcattaagaa aaatacgttt
      721 tttagagtgt gaaaataacg gagtatgtaa aaattcctcc agcgcactca cttgtctgaa
      781 tactgcactt tgggtcaacg ctaactcctc tgccgctctg gtataacttt catgacgtgc
      841 gacgacctca aaagtcacca gcagttccgt tttaggtatt tttcctcgca taatgccgtc
      901 ccttaaaaat agtttaaacc atcaaaaata cattacttat tacgaagtaa tggtaaccaa
      961 agcagcaata aataatttat aagaatcatg attgacattt ataacccagt aagcctggcg
     1021 tgacaaatat cttaatcaga taataataat ccgaaaagca cagcgccttt cgaattatcc
     1081 ggaatcacat taagtgaaaa ttattaaatt gatatcattt atcatatctg tggatattat
     1141 gataaacaat caacaaagat tttatttttg atttatagca tatccctcta agaatttttg
     1201 cgatgctgca atatccgggg taaagacccg atcctggctg acaaatggca cgacttgacg
     1261 ataagcggtg aacatcgctt ttgttgattt acttaacgct acggtcaaac gctgggaagc
     1321 cagtgcatca ggtaacgatt cgaaattcgc cgtcgttctt attcgtggct tgcggtcggt
     1381 tgcggcaatc cacagtggct gtggtcatgc agtcaagcca gcggtcagat ttcgaccgtg
     1441 gttaacattt aaggcataaa aaaggccatc atacgatggc caaccatgtc gaaaccgaca
     1501 gcccttcgcc attcctgtag agggaagcgg cgagggctat ttcttattac agatatttat
     1561 ttcagaacag ttttcagtac ttcaccgata tccgccaggc tgcgaacggt tttcacgcct
     1621 gcggcttcca gagcagcgaa tttctcatcc gcagtccctt tcccaccggc aatgatggca
     1681 cccgcgtggc ccatacgttt gcctttcggc gcagtcacac cagcgatgta acctacaact
     1741 ggcttggtaa cgtgctcttt gatgtacgca gccgcttctt cttcagcgct accgccgatc
     1801 tcaccgatca tcacgatcgc ttcggtctgc ggatcttttt cgaacatttc gaggatgtcg
     1861 ataaagttag agccagggat cgggtcaccg ccgataccga cacaggtcga ctgaccgaaa
     1921 ccgtaatccg tggtctgttt aaccgcttca taggtcagtg taccggaacg ggaaacgata
     1981 cccactttac ccggtttgtg aatgtggccc ggctggatac cgattttgca ttcacccgga
     2041 gtgataacgc ctgggcagtt cgggccgatc atacgaacgc cagcttcgtc cagtttcact
     2101 ttcacagtca gcatatccag cgtcgggatg ccttcagtga tggtgataat cagtttgatg
     2161 cctgcatcga tggcttccag aatggagtct ttgcagaacg gtgctggtac gtagataaca
     2221 gaagcggtag cgccagtagc agcaacagct tcacgcacgg tgttgaacac cggcaggccg
     2281 aggtgggtgg tgccgccttt acctggggtt acaccgccaa ccattttagt gccgtatgca
     2341 atggcttgtt cagagtggaa agtcccctgg ctaccggtaa agccctggca gataaccttg
     2401 gtgtttttat caattaaaat ggacattatt tcccctccac tgcggcaaca acctgctgag
     2461 ctgcatccgt cagacctttt gctgcaataa tattcaggcc gctgtcagcc agtttcttcg
     2521 cgccgagttc ggcgttgtta ccttccagac gtaccacgac cggtacgtta acacccactt
     2581 ccgctaccgc gccgatgata ccgtcagcga tcaggtcgca acgaacgata ccgccgaaga
     2641 tgttaaccag aacggctttc actttgtcgt cagagaggat gattttgaat gcttcggtta
     2701 cacgttcttt ggttgcgccg ccgccaacgt ccaggaagtt agccggttcg ccgccgtgca
     2761 gtttaacgat gtccatcgta cccatcgcca gacctgcgcc gttaaccata caaccgatgt
     2821 taccgtccag cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca cgcggatctt
     2881 cctgtgactg gtcacgcatt tcgcgcagat caggctggcg gaacagtgcg ttgccgtcag
     2941 cacccagttt gccgtcgagg caaatcagat cgccctgttt agtgatgacc agcgggttga
     3001 tttcgatcag cgccaggtcg cgctccagga aaatggtcgc caggcccatg aagattttgg
     3061 tgaactgctg aaccagttta ccttccagac ccagtttgaa cgccagctcg cgtccctgat
     3121 acggcatcgg gccagtcagc ggatcaagcg caactttatg gatcaggtgc ggagtttctt
     3181 ccgccacttt ttcgatttcc acgccgcctt cggtggaggc cataaagacc acacgacggg
     3241 aactacggtc aacaacggca ccgagataca gctctttagc gatatcggtc gctgcttcaa
     3301 ccagaatctg gttaaccggt tggccattgg catctgtttg atacgttacc agacgcttgc
     3361 ccagccagtt ttctgcaaaa gcacggatgt cttctttgct gtttaccact ttcacaccgc
     3421 ccgctttacc gcggccacca gcgtgaacct gacatttcac tacccacgga ccggcaccga
     3481 tttttgaagc ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc accggtgctg
     3541 gtaagccata gcgggcaaaa agttgttttg cctgatattc atgtaagttc atgtgttctg
     3601 tccatccttc agtaatcgtt atcttttaaa ccgtagaccg acaccctatc cacgtcggtc
     3661 tacagtgcag gtgaagctta aactactaca cgtccagcag cagacgagtc ggatcttcca
     3721 gcaactcttt gatcgttacc aggaagccca cggattcgcg accatcgatc agacggtgat
     3781 cgtaggacag cgccaggtac atcatcggca ggatctcaac ctgaccattc accgccatcg
     3841 gacgatcttt gatagcgtgc atacccagaa ttgcgctctg cggcgggttg atgatcggcg
     3901 tagacatcag ggaaccgaac acaccaccat tggtgatggt gaagttacca ccggtcagat
     3961 cttcaaccgt cagcttgccg tcacggcctt taactgccag ctctttgatt ttcttctcga
     4021 tgtctgccat gcctagggta tcgacatcac gcagtaccgg cgtcaccagg ccgcgcggcg
     4081 tagaaaccgc catgctgacg tcgaaatagt tgtggtaaac cacatcatcg ccgtcgatag
     4141 aagcattcac ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg tagaaggaca
     4201 taaagcccag acgaataccg tgacgttttt caaacgcttc accgtactgc ttacgcagat
     4261 ccataatcgg cttcatgttg acttcgttga acgtggtcag catagcggtg gagtttttcg
     4321 cttcaagcag acgctctgcc acacgcttac gcaggcgagt catcggcaca cgtttttcgc
     4381 tacgtgcagc cagagccggt tgcgccgccg gagcagccgc tgccggagca gactctttcg
     4441 ccggggcttt cgccagatgt ttttccacat cttcacgagt cagacgacca cccacaccgg
     4501 tgcctttaat ggcgctggcg tcgaggttgt gttcagccag caggcgacgg atcgccgggc
     4561 ttaacgcatc gttgttttgc tcttccagag acgcctgctg gcgttgcgcc ggagtggacg
     4621 ctttctcttc agatttggcg ctggtttctt taccggtgct gttgccttca cgcaggcgac
     4681 caaggatctg acgagacgtt accgttgtac cttcatcttc cagaaccgca tccagaatgc
     4741 cgtctgctga tgccggtact tccagtacca ctttgtcagt ttcgatttct accagcactt
     4801 catcacgtac gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg gcatcggcta
     4861 cggattcagg caggtcaggg accagaatat ctacgctact cattgtgtat cctttattta
     4921 ttcgacgttc agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa cggacatata
     4981 ccctaccgcc ggagaggcgg aggccgggcg gcctgcataa cgcagagaag ccccaaacgg
     5041 aatcacttca cggaaatgat gctggctgca gtaccatgcg ccctggttga gcggctcttc
     5101 ctggcaccag acaaaatcct tgacgtgagc aaactgctgc aacacttcct gcatcgcttt
     5161 atgcgggaac gggtagagtt gctcgatacg cacaatggcg acatcgtgtt gattgttctt
     5221 acgacgctgt tccagcaggt cgtaataaac cttaccagaa cacatcacta cgcgcttcac
     5281 gcccttcgga tcaagctcgt cgatttcacc gatggctggc aggaaggtgc cgttcgccag
     5341 ttcttcgagg ctggaaaccg ccagcggatg acgcagcagg gatttcggcg acatcacgac
     5401 cagcggacga cgcatcccgc gcagcgcctg acgacgcagc atgtggtaaa cctgtgccgg
     5461 ggtagacggt acgcacacct gcatgttttg ctcagcgcaa agttgcagat aacgttccag
     5521 acgcgcggag gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca acatcaccag
     5581 accacacatc cggccccatt tctgttcgcc agaggagatg aactggtcga taaccacctg
     5641 agcaccgtta gcgaagtcac cgaactgtgc ttcccagata gtcagagtgc gtggttctgc
     5701 ggtggcataa ccgtattcaa acgccagcac ggcttcttct gacagtacgg agtcccagac
     5761 acggaacgcg ccctggccgt tatggatgtg ttgcagcggc gtgtaagtgg aaccgttaga
     5821 ctggttgtgg atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt cttcacccga
     5881 caggcgaacc ggaatgcctt catcaaccag cgtggcgtaa gcgaggtttt ccgcgccgcc
     5941 ccagtcgaac agtttctcac cggctgccat tgcctggcga tcgccataaa tcttggcaac
     6001 gcgagactgc atttcaactg cttccggcac cgtgctgatg cgtttagcca gttcctgcag
     6061 gcgcttcatc tcaactttgt tcgggtactc ttcgtcccat tcgtggttga ggtacggtga
     6121 ccaggtgaaa gagtgcatgt tcatcggacg ccactctgct accacgcaat cgccagcatc
     6181 cagcgcatcg cggtacaggt taaccatctc ggtggcatct tccagcgtcg cgactttttc
     6241 ctgctccagc ttgtcagcgt agattttgcg cggcgtcgga tgttttttga ttttctgata
     6301 catcagcggc tgggttgcgc tcggctcgtc ggcttcgtta tggccatgac ggcggtagca
     6361 caccaggtcg atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg ccagacgggt
     6421 cacaaaggca acggcttccg gatcgtccgc gttaacgtgg aaaatcgggg cctgaaccat
     6481 cttaccgata tcagtacagt acggtgtaga acgagcatcc agcggattag aggtggtgaa
     6541 gccaacctgg ttgttgataa cgatacgtac cgtaccgcca acttcataac cacgcgcttt
     6601 cgacatgttc agggtttcct gaaccacgcc ctgcccggtc actgcggcgt caccgtggat
     6661 ggtaattggc agcactttgt tgctgctcgg ctcatcaagt ctgtccagac gggcacgaac
     6721 agaaccgata actaccgggc tgacaatctc aaggtgagac gggttaaacg ccagcgccag
     6781 gtgcaccagg ccgccatcgg tctggaagtc agacgagaag cccatgtggt atttcacgtc
     6841 gcccgtgccg aggtgttctt tatgtttacc ggcaaactcg tcgaacaagt cttgcggttt
     6901 tttacccagc acgttcacca gcacgttcag acgaccacgg tgcgccatcc cgagaaccac
     6961 ttcgcgggtg ccgctgttgc cagcgtggcg gatcatctct ttaagcatcg ggattaacgc
     7021 gtcaccgcct tccagcgaga agcgttttgc accaggaaat tttgcaccga ggtaacgttc
     7081 aaggccttca gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc tattgaaagt
     7141 cgcgcgacca gactcgatac gctgttggat ccagcgtttt tcttcggtgc tggtaatatg
     7201 catatactcg gcaccaatcg ggccgcagta ggtttgcttg agggcttcca gcagctcgcc
     7261 gagtttcatg gtttctttgc cgctggcaaa tgaaccgacg ttgaaggtct cctggaagtc
     7321 tgcttcggtc agatcgtgga aagacggatc cagatcggcc actttatctt gctgccacag
     7381 tcccagcgga tcgagattcg catgctggtg accacggaag cggtatgcgt taatgagctg
     7441 caggacttta acctgcttca cattggtgtc agggtcggag atcgttgaag agtaacgtga
     7501 agcgtctttc gccaggcggc ggaaatattc acgcgtttga gagtggaatt gatccggttt
     7561 gactcccgta ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa ccgagtcagg
     7621 atcggttaag aagtcttcat agagctgttc tatccagctc tggtttgcgc cagagaggta
     7681 agaagagtcc aaccaggctt tcaaagcgct gttctgcatc gtgatccctt aagcatcttt
     7741 tttatgctta cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc ggggttcact
     7801 tgcgagctct tgtctacgta gtggctcgcg aaggaacctt taaaaactgt cttagtgtca
     7861 ggggcagttt ttaaagattt cctgcatttc ttttgtaggc cggataaggc attcacgccg
     7921 catccggcgc tgtttgcctg atgcgacgct tgcgcgtctt atcaggccta cgatttctgc
     7981 atttcttttg taggccggat aaggcgttca cgccgcatcc ggcactggtt gcctgatgcg
     8041 acgcttgtgc gtcttatcag gcctgggttt acgcattacg ctgcaacaac atcgacttga
     8101 tatggccgat ggcgcgcgtc gggttcagcc ccttcggaca tacactgacg cagttcatga
     8161 tgctgtgaca gcggaataca ctgaatgcat cgctcaaacc gtcgaggcgg ctgtcagtct
     8221 cggtatcacg gctatcgatc aggaaacgat atgccgctaa caagcctgcc gggccgataa
     8281 acttatcggg attccaccag aaagacggac aagaggttga acaacatgcg cagagaatac
     8341 attcatacag cccgtcgagt ttttcgcgct gctctggcat ctgtaaatgc tcgcgagctg
     8401 gcggattttg tccattattc aacaggtaag gcttaatttt ctcatattgc gcatagaatt
     8461 gtcccatgtc taccaccaaa tcgcggatca ccggtaaacc tggcagcggg cgaatcacaa
     8521 tcttcttgcc cggctggttg agtgccgaaa tcggggtaat acaggccaga ccattcttgc
     8581 cgttcatgtt cagaccgtcg gaaccgcaca caccttcacg gcaggagcga cggaacgaca
     8641 ggctgggatc tttctctttc agctggataa gcgcatccag cagcatcatg tcacgacctt
     8701 cttccgcttc cagggtgtaa tcctgcatac gcggagcatc atcaacatcc gggttatagc
     8761 gataaattga aaactcgagt ctcattttcc tgtctccgca ttagtaagta cgaatcttcg
     8821 gcgggaatgc cgggcgcagt ttcggttcca tgttgacgct tcggcgcgtc atggattccg
     8881 actctggcag atacagggag tggcacagcc agttttcatc atcacgatcc gggaagtcga
     8941 agcggctatg cgcgccacgg ctttcggtac ggaagttggc agaaacagcc gttgcatacg
     9001 ccgtttccat caggttatcc agttccaggc actcaacgcg ctgggtgttg aactcgctgg
     9061 aagtgtcatc cagacgggca tttttcagac gctcgcggat cactttcaac tgctcaagcc
     9121 ctttcgccat cgcatcacct tcacggaaga ccgagaagtt atgctgcata cattcttgca
     9181 gcgctttacg aatcgccacc ggatcttcac cgttacgatt attgttccag cggttcagac
     9241 gatccagaga cgcttcaaca tcagactcgc tggcatcgcg cagtgcgccc tgctcggcga
     9301 tagactcttg cagatgcaga cctgccgcac gaccaaagac caccaggtcc agcagcgagt
     9361 tgccgcccag acggttagcg ccgtgtaccg atacacaagc gatttcacca acggcaaaca
     9421 gccccggaac aaccacatct tcgcctttct cgttcacagt cagcgcctga ccggtcactt
     9481 tggtcggaat accgcccatc atgtagtgac aggttgggat aaccggaatc ggctctttca
     9541 ccggatcaac gtgagcgaag gtacgggaga gttccaggat acccggcaga cgggattcga
     9601 gaacttcttt acccaggtgg tcaagtttca gttttgcgtg tggcccccac ggaccatcac
     9661 agccgcgacc ttcacggatt tcgatcatga tggaacgcgc caccacgtca cggcccgcca
     9721 ggtctttggc gttcggcgca taacgctcca taaagcgttc gccatgtttg ttcagcagat
     9781 aaccgccttc accacggcaa ccttcggtga ccagtacgcc cgctcctgca atgccggtcg
     9841 ggtggaactg ccacatttcc atatcctgta ctggtacgcc tgcacggata gccatgccga
     9901 cgccgtcgcc agtgttaatg tgggcgttgg tggtggactg ataaatacgc cctgccccgc
     9961 cagtcgccag cacggtagcg cgggctttga aataaaccac ttcaccagtt tcgatgcaca
    10021 gtgcggtaca accgaccacc gcgccatcgg cgtttttcac cagatccagc gcataccatt
    10081 cggagaaaat agtggtgtgg tttttcaggt tctgctgata aagggtgtgc aacagtgcgt
    10141 gaccggtacg gtcagctgcc gccgcagtgc gtgccgcctg ctcgccgccg aagtttttcg
    10201 actggccgcc aaacggacgt tgatagatac gaccatcatc aaggcgcgag aacggcaggc
    10261 ccatgtgttc gagctccaga atcgcttccg gcccggtttt acacatatat tcaatcgcgt
    10321 cctggtcacc gatatagtcc gaacctttta cggtgtcgta catatgccat tcccagttat
    10381 cttcatgggt attacccagc gcaacggtaa tgccgccttg cgcagaaacg gtatgggaac
    10441 gggtcgggaa gactttagag agcagcgcac aggtctgacc gctctgggaa atttgcagcg
    10501 ccgcgcgcat acctgcgcca ccggcaccaa tcacaactgc atcaaattct ctgactggca
    10561 atttcatcac acaccccaca ccacaacgaa tccataaatc acgtaaacca ccagtgcaac
    10621 gacaatcacc agttgcagca tcaggcgcaa agccagcggt ttaacgtagt cggtcaacac
    10681 ctgccacatg ccgatccagg catggatcaa gatagaaaac agcgccagca gggtgaacac
    10741 tttggtgaac gcagaggcga agaaaccgat ccagacttca tatgtcagct cgccactggt
    10801 agcgaaaaaa ccgaccatat aaatgatgta gagcgtcagg acgatagcgg tagcgcgaac
    10861 gaggataaaa tcatgtacgc cattgcgtcc taatgcggag gcgttgctta ccatacgagg
    10921 actcctgcga gaagtgaaag cacgacagta ataacaaagg agattttggc ggagcgttta
    10981 cccgcttcga atgtttcttc cagatagcca aaatccatca tcatgtggcg aatacctacg
    11041 acgacgtgat acgccagagc ggtaaggatg ccccacatga taaatttgac gaagaagctg
    11101 cccataatcg cggaagcttg ctcgaaacct tcaggggaag agaggctggt acccagaagc
    11161 cacagcagga tgcccactgc aacaaaggtg atcacaccgg aaacgcgatg gagaatggac
    11221 gctatcgccg tgatggggaa ccggatggtc tgtaggtcca gattaacagg tctttgtttt
    11281 ttcacatttc ttatcatgaa taacgcccac atgctgttct tattattccc tggggactac
    11341 gggcacagag gttaactttc tgttacctgg agacgtcggg atttccttcc tccggtctgc
    11401 ttgcgggtca gacagcgtcc tttctataac tgcgcgtcat gcaaaacact gcttccagat
    11461 gcgaaaacga cacgttacaa cgctgggtgg ctcgggattg cagggtgttc cggagacctg
    11521 gcggcagtat aggctgttca caaaatcatt acaattaacc tacatatagt ttgtcgggtt
    11581 ttatcctgaa cagtgatcca ggtcacgata acaacattta tttaattttt aatcatctaa
    11641 tttgacaatc attcaacaaa gttgttacaa acattaccag gaaaagcata taatgcgtaa
    11701 aagttatgaa gtcggtattt cacctaagat taacttatgt aacagtgtgg aagtattgac
    11761 caattcattc gggacagtta ttagtggtaa acaagtttaa taattcggat tgctaagtac
    11821 ttgattcgcc atttattcgt catcaatgga tcctttacct gcaagcgccc agagctctgt
    11881 acccaggttt tcccctcttt cacagagcgg cgagccaaat aacaaacggg taaagccagg
    11941 ttgatgtgcg aaggcaaatt taagttccgg cagtcttacg caataaggcg ctaaggagac
    12001 cttaaatggc tgatacaaaa gcaaaactca ccctcaacgg ggacacagct gttgaactgg
    12061 atgtgctgaa aggcacgctg ggtcaagatg ttattgatat ccgtactctc ggttcaaaag
    12121 gtgtgttcac ctttgaccca ggcttcactt caaccgcatc ctgcgaatct aaaattactt
    12181 ttattgatgg tgatgaaggt attttgctgc accgcggttt cccgatcgat cagctggcga
    12241 ccgattctaa ctacctggaa gtttgttaca tcctgctgaa tggtgaaaaa ccgactcagg
    12301 aacagtatga cgaatttaaa actacggtga cccgtcatac catgatccac gagcagatta
    12361 cccgtctgtt ccatgctttc cgtcgcgact cgcatccaat ggcagtcatg tgtggtatta
    12421 ccggcgcgct ggcggcgttc tatcacgact cgctggatgt taacaatcct cgtcatcgtg
    12481 aaattgccgc gttccgcctg ctgtcgaaaa tgccgaccat ggccgcgatg tgttacaagt
    12541 attccattgg tcagccattt gtttacccgc gcaacgatct ctcctatgcc ggtaacttcc
    12601 tgaatatgat gttctccacg ccgtgcgaac cgtatgaagt taatccgatt ctggaacgtg
    12661 ctatggaccg tattctgatc ctgcacgctg accatgaaca gaacgcctct acctccaccg
    12721 tgcgtaccgc tggctcttcg ggtgcgaacc cgtttgcctg tatcgcagca ggtattgctt
    12781 cactgtgggg acctgcgcac ggtggtgcta acgaagcggc gctgaaaatg ctggaagaaa
    12841 tcagctccgt taaacacatt ccggaatttg ttcgtcgtgc gaaagacaaa aatgactctt
    12901 tccgcctgat gggcttcggt caccgcgtgt acaaaaatta cgacccgcgc gccaccgtaa
    12961 tgcgtgaaac ctgccatgaa gtgctgaaag agctgggtac caaagatgac ctgctggaag
    13021 tggctatgga gctggaaaac atcgcgctga acgacccgta ctttatcgag aagaaactct
    13081 acccgaacgt cgatttctac tctggtatca tcctgaaagc gatgggtatt ccgtcttcta
    13141 tgttcaccgt gatcttcgct atggcacgta ccgttggctg gatcgcccac tggagcgaaa
    13201 tgcacagtga cggtatgaag attgcccgtc cgcgtcaact gtatacagga tatgaaaaac
    13261 gcgactttaa aagcgacatt aagcgttaat ggttgattgc taagccgttt actttccgga
    13321 cccgccttta atagagcggg ttctttttta tatggctgaa cataataaat tgtaaaaatt
    13381 aaaccagatg caaataccca ttaataaggt gaaataatgt gacaagtcac atactgagca
    13441 gaatgataag tttattttta tactttcctg ccggtagcat tgttaaccca atgaaaatta
    13501 tgcatatcca tcccacccca tctttcatct cattaaacaa taaatttatg cactcaatta
    13561 ttccattctt tctataacaa gctttttaat attgttaaat aaaaccaaat acattcaact
    13621 gtcagttaat atattcttgc ctcgcaacac tctatctcat tatttaaagg actcagctat
    13681 gtttaaagga caaaaaacat tggccgcact ggccgtatct ctgctgttca ctgcacctgt
    13741 ttatgcggct gatgaaggat ccggtgaaat tcactttaaa ggtgaagtta ttgaagcacc
    13801 gtgtgaaata catcaggatg atattgataa agaggttgaa ctcggtcagg tgaccaccag
    13861 ccacattaat cagtcgcatc acagcgatgc cgttgctgtc gacctgcgct tagtcaactg
    13921 cgatctggaa aactccaaca acggttccgg cggcaagatt tctaaagttg cagtcacctt
    13981 tgacagctca gcgaaaacca ccggcgcaga tccgattctc aacaacacca gcacgggtga
    14041 agcgaccggt gtcggcgtgc gtttaatgaa taaagatcaa agcaatatcg ttttaggcac
    14101 cgctactcca gatatcgacc tggcaccaac ctccagcgaa caaacgttaa atttctttgc
    14161 gtggatggaa caaattgatc aggctacgcc cgtaacacca ggtgcagtta cggcgaatgc
    14221 aacctacgtg ctggattata aataagtatc actttggcgg cgctatcgcc gcctttcttc
    14281 aaatatttat caggtatgga caccgtgaat atttatcgac tctcttttat atcctgccta
    14341 gtcatggcga taccttccgc attggcggtc gaattcaacc tgaatgtcct cgataaatcg
    14401 atgcgagatc gcattgatat ttcattatta aaggaaaaag ggggcattgc tcccggtgaa
    14461 tattttgtta gcgttgcagt aaataacaac caaatcagta acggacaaaa aattgactgg
    14521 aaaaagaacg gtgaccaaac cattccgtgt attaatgatt tactggtaga taaatttggt
    14581 ttaaaacctg aagttcgtca gtcgttacca cggttaaacc agtgcgtcga ttttagctcc
    14641 cggcctgaaa ttctcttcat ttttgatcag gctagccagc aactgaatat cactattcca
    14701 caagcctggc tggcgtggca ctcagataac tggactcccc cctcaacatg gaaagaaggt
    14761 gtcgccggcg tcctgatgga ctacaacctg tttgccagca gctaccgccc acaggatggt
    14821 agcaacagca ctaacctgaa cgcctatgga acagccggaa ttaacgcggg agcatggcgt
    14881 ttacgcagtg attaccaatt gaatcagacc gatagcgatg ataaccatga acagtcaggc
    14941 gaaatatcac gcacctatct ttttcgtcca ttaccgcaat taggttcaaa attaaccctt
    15001 ggtgaaacgg atttcagttc caatattttc gacggctttt cttataccgg cgcggcactg
    15061 gcaagtgacg atcggatgtt accgtgggag ctacgcggct acgccccaca aatcagcggc
    15121 attgcacaaa ccaacgccac cgtcaccgtg agtcactcag ggcgagttat ttaccagaaa
    15181 aaagtcctac caggtccgtt tatcattgat gaccttaatc agtccgtcca gggtacgttg
    15241 gatgtcaaag tgacggaaga agatggtcgg gtgaacaatt tccaggtttc ggcagcatcg
    15301 acgcctttcc ttacgcgcca ggggcaggtt cgctataaac ttgctgccgg tcagccgcga
    15361 acatctatgt ctcatcaaac tgaaaatgaa acctttttta gcaatgaagt ttcctggggg
    15421 atgctgtcaa atacctcact gtacggcggc ctgctgattt cgggtgacaa ctaccattct
    15481 gccgcaatgg gtatcgggca aaatatgctc tggcttggtg cattgtcgtt tgatgtcacc
    15541 tgggccagta gtcagtttga tactcagcag gacgagcggg gcttaagtta ccgctttaat
    15601 tacagcaaac aagtggatgc cactaacagc acgatttcgc tcgccgctta tcgcttctca
    15661 gatcgtcatt ttcacagcta cgccaactat ctggatcaca aatacaacga caacgatgcg
    15721 caggacgaaa aacagacgat cagcttatcc gtggggcaac cgattacccc actgaacctc
    15781 aatctgtacg ccaacctgct gcatcaaacc tggtggaatg cagacgcctc cacgaccgcc
    15841 aacatcacgg cgggttttaa tgttgatatt ggtaactgga gagatatctc gatctcgacg
    15901 tcattcaata caacccatta cgaagataaa gatcgcgaca atcagattta cctgtcgatt
    15961 tcgctcccct tcggtaacgg ggggcgggtc ggttatgaca tgcaaaacag taatcacagc
    16021 accacgcacc gcatgtcgtg gaatgatacg ttagatgaac gtaatagctg gggcatgtct
    16081 accggactgc aatccgaccg tccggataat ggagcccagg tgagcggtaa ctatcagcac
    16141 ctgagttcag cgggtgagtg ggatatttct ggtacctatg ccgccaatga ttacagttcc
    16201 gtcagcagca gctggagcgg ttctttcacg gcaactcaat atggtgcggc gttccatcgc
    16261 cgcagctcca ccaatgaacc acgcctgatg gtcagcaccg atggcgtggc agatattccg
    16321 gttcagggca atctcgacta caccaaccat tttggcattg cggtggtgcc gttgatttcc
    16381 agttaccagc cttccaccgt ggcggtgaac atgaatgatt tacccgacgg cgtaacagtg
    16441 gcagataacg ttatcaaaga aacgtggatt gaaggcgcga taggttacaa atcgctggct
    16501 tcccgttccg gtaaagacgt taacgtcatc attcgcaacg ccagcggtca gctccccccc
    16561 ctcggtgcgg atatccgcca ggaggatagc ggcattagcg tgggaatagt tggtgaggaa
    16621 ggacacgcct ggttaagtgg agtcgctgaa aatcaacaat ttaccgtgat ctggggtgat
    16681 agccagcgtt gctcgatcca tcttcctgaa catatggaag acaccgcaaa tcgcctgatt
    16741 ttaccttgtc attaattaag gaaatgaaaa tgacatttat gaaaggacta ccccttttgt
    16801 tgttggttgc cagtctgtgc agctacgctg cgctacaacc cgatcgcact cgcattgtgt
    16861 ttaacgctaa tgataaagcc accagcctgc gagtggataa tcgtagcgat aaactcccct
    16921 acctcgctta ttcctggctc gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg
    16981 ccctgccacc gatacaacgt ctggaaccga aagcaacaac gcaggtgcgg atagtcaagc
    17041 aagcctcgac cgcgaaatta cctggcgatc gcgaaacgct atttttctac aatatgcgtg
    17101 aaattcctcc cgccccagaa aaaaatagcg accatgccgt tctgcaagtc gccattcaga
    17161 gtcgtatcaa ggtattctgg cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg
    17221 aactgcagtt acaggtaagc cagcaggcca accaactgac actgaaaaat cctaccgcgt
    17281 attatctgac cattgcctat ctggggagaa atgaaaaagg cgttctcccc ggcttcaaaa
    17341 ccgtgatggt tgcgcctttt agtacggtca acacaaacac cggaagttat agcggcaggc
    17401 agttttatct cggttatatg gatgattacg gcgcgttacg catgacgacg ctcaactgca
    17461 gcggacaatg ccgtttacaa gcagtggagg cgaagaaatg agtgctggca agggagtgtt
    17521 gctcgtcatc tgtctggtat ttctgccgtt gaagtctgcg cttgccctga actgctactt
    17581 tggttcatcc ggtggttcgg tagaaaaatc agaagtgatt caaccttttg ctgtgccagg
    17641 taatgccaaa cccggcgata agatctggga atctgacgat attaaaattc ccgtctattg
    17701 tgacaacaat accaacggca actttgaaag cgaacatgtc tacgcatggg taaaccccta
    17761 cccgggggta caggatcgtt attatcaact cggcgtgaca tacaatggtg tcgattacga
    17821 tgccagtctg gggaaaagcc gcatcgacac caaccagtgt atcgacagta agaatattaa
    17881 catttatacc cctgagcaga ttatcgcgat gggctggcag aacaaaatat gctcgggtga
    17941 ccccgctaat attcacatgt cgcgaacctt tctcgctcgt atgcggttat acgttaagat
    18001 tcgcgaaatg ccgccgcatg attatcaaag tacgcttagc gactacatcg tcgtgcaatt
    18061 tgatggtgcc ggtagcgtta acgaagaccc taccgcccaa aacctgaaat accatattac
    18121 tggtctggaa aacattcgtg tactggattg cagcgtcaat ttttctattt caccagaaac
    18181 acaggtgatt gattttggta aatttaacct gctggatatt cgccgccaca caatgtcgaa
    18241 aacattcagt attaagacga caaaatcaca aaatgaccag tgcaccgacg gatttaaggt
    18301 cagctcctcc ttttataccg aagaaacgtt ggttgaagaa gataaagcat tgttgattgg
    18361 taatggtctg aagctacgtt tgctggatga aaacgcctcg ccttatacct tcaataaata
    18421 tgctgaatac gccgatttca ccagcgatat gctgatctac gaaaaaacct atacggctga
    18481 actttcgtcc attccaggta cccccatcga ggctggcccc ttcgatacag tggtgctttt
    18541 taagattaac tataactgat ttgccatgat gccctttgca gccggaaagg gcatctcttt
    18601 cagaaaattg ctgattttcc ccttttcctt gattgctgtt attttttcct ggcccggctt
    18661 tctttatgct gtatcgatac accaaagaaa aaataatgag ggaacgaagg atgccaattt
    18721 tgcagtgggg aatgttatgt gtgctctcac ttctcctttc tattggtttc ctcgcggtac
    18781 atctcccggc ggcgctatta cttggaccga tgatcgccgg gatcatcttt agtatgcgcg
    18841 gaattacttt acaactcccc cgctccgctt ttctcgccgc gcaggccatt cttggctgta
    18901 tgattgcgca aaacctcact ggttcgattc tcaccacgct ggccgtcaac tggccgatcg
    18961 tgcttacaat tttactggtg acgctgcttt ccagcgccat tgttgggtgg ctactggtgc
    19021 gctatagctc attaccagga aatactggtg cctggggttc ttctcctggc ggcgcggcgg
    19081 caatggtcgc tatggcgcaa gattacggcg cagatattcg cctggtggcg tttatgcaat
    19141 atctgcgagt gctgttcgtc gcaggggccg ctgttctggt cacccgcatg atgctgggcg
    19201 ataacgctga agcggttaac cagcagatcg tctggtttcc gccagtgagc attaatctcc
    19261 tgcttacgat tttgctcgcg gtcgttgctg gtacggcggg atgcttgcta cgtcttcctt
    19321 ccggcacgat gctcatcccg atgctggcgg gcgcagtgct ccagtctggt cagctcatca
    19381 ccatcgaact accagaatgg ctgctggcga tggcgtatat ggcaattggt tggcggattg
    19441 gtcttggttt cgataaacaa atattactgc gggcattgcg cccactgcca caaatcctgc
    19501 tgtcgatttt tgctctgctg gctatttgtg caggtatggc gtggggactg acccggttta
    19561 tgcatattga ttttatgact gcctacctcg ccaccagccc cggcgggctt gataccgtgg
    19621 cggtcatcgc cgcagggagc aatgccgata tggcgcttat tatggcgatg caaaccctgc
    19681 gcctgttcag tattttgctg acgggacctg ccattgcgcg gtttatttca acctatgcgc
    19741 cgaaacgtac ggcctagtgc tgacagcctg ggcaccagta aaacgggcga gatgacagcg
    19801 tggttttctc aatgatgctg ccacagcgtt cacacggttc gccatcacga tgaaaaacct
    19861 taaagcgaaa caacgcccca tgatgtttat tctcatccac ctgcccccgc gtagcgtaag
    19921 aaagtcgagg aatatccagt aacgcgtgtg cgagtgcatc tagtcgcgcc gcactgagat
    19981 ctttcgcttt atgattgccg gttaacccaa cctgccagag gatctccacc cgcaaataat
    20041 tgccaagccc agccagaaac gcctgatcga gtagtaatcc agcaaactga cggttacgaa
    20101 agcgcggcga caataaccgt tcctttacca cctccggcgt cagattcgga tccagcacat
    20161 cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg ttccggggtc aacatctcaa
    20221 tatccgaggc gctataaagc agaatggttt tgtcagccgt ttgcagtttt acccgcaata
    20281 ctcgcgtggt ctgcggctct tcgccggttt caaccacgcg ccagacgccg taaagctgat
    20341 tatggctgta gagcgttaag tcgttggaaa aatgagttaa caacgcctta ccacgcgttt
    20401 ccacatgggt aacgtgttga ccgataagtc gtgattgata agattttaac tgcgggaagg
    20461 caaaccagac atcagttagt ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac
    20521 ggcggatctc cgggccttca ggcatggtga tatccttttt tcattcttta ttaaacgggg
    20581 ttatgctgcg acaacaatcc ccttttcggc aaatgtagag cgtagtcggc gggcgaacgc
    20641 cagtgcgtgc tcgccatcgc catgcaggca gaccgtttgc gccgtgaccg ttgcccattc
    20701 gccggtgatg cttttgactc tgccgtgttg caccatctcc aacgtttgct ccagcgcctg
    20761 ttcttcgttt tcaatcaacg cgcctggctg gcttcgcggc accagcgaac cgtctgcctg
    20821 ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg
    20881 aatcagctcg cttcctgcca gcccgacgag aatcaatgcc ggatcgcaag cgtataccgc
    20941 tctggcgatg gcgtctgcca gttgtgcctc tttcgccgcc tggttgtata acatgccgtg
    21001 cggtttgaca tgacacatta cgccgccttg cgcacgggta atcgctgcca gcgcgccaat
    21061 ttgatacagc gtctgggcgt aaacggtttc tggcggcagc tgcatggctc tgcgaccaaa
    21121 attttccctg tcgggaaaac tcggatgagc gccaatcgca acaccatttt ttattgcttc
    21181 acgcacgcaa gcctgcatgg tttgcgcatc gcctgcatga aatccacaag caatattggc
    21241 agaggaaacc agcgttaata gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt
    21301 caggtcaatt ttcattgtgc agccgccacg ctaattgttc gaaataacgt tgttgatctt
    21361 gccgcgcttt cagtgcctct tccagtgaac actggacaaa atgaatcggc tgaccgagcg
    21421 gaatttgcgc cagatggtac atatcagcct caatgataca ggcaatacgc ggataaccac
    21481 cggtggtctg tgcgtcgttc atcaatacaa tcggctggcc gttatgcggc acttgcacca
    21541 cacccggcaa caaaccgtga gataacagtt cgcgatcggt ggtgcgtttt aaaatttgcc
    21601 cctgtaagcg atagcccatt cggttacttt gcgagctaag ctgccagggc gaacgccaga
    21661 atgcatcctg cgaggcgcga tcgaactcat gatattccgg ccccggcaag gcgcgaatgc
    21721 ggttgcccca cagcagctgt ttaacgccct gcgcttccat aaaatcacgc ttcgctttgc
    21781 caatcgggag tcggtcacca tccttcagta aacggccttc cagcccgcca atccccactt
    21841 tgagatcggt gctgcatgac cccattaccg gcggaacatc aataccaccc gcgaccgcca
    21901 gataactgcg catcccgtgc tgcgggcgtt taagcgttaa acgctggcct gctttcatcg
    21961 gcaatcgcca gccagtccag acggcattat catccagccg cgcttcgcaa cctgcccccg
    22021 tcagggcaaa ccacccatca gtttcgaact ctaccgttaa ctgaccgagc gtgatctcca
    22081 gcgcgggggc attggcgtca ttacccacca gcaggttagc aatgcgtaac gcaggcatat
    22141 ccagtgcgcc gcagtggctg atacccgact ggcgaaaacc gtgacgaccg ccatcctgca
    22201 cagtggtata catgcccgca cgaataatct tcagcatact ccctccttct gcggtacaaa
    22261 gcgcacgctg tctcccggac gtaataagat gggttcgtca cgcgccggat caaacaggct
    22321 gagtgaggta tgaccaatca actgccagcc acccggcgtt gccagcggat aaacaccagt
    22381 ctgcggcccg ccgatcccga cagaacctgc cggaacgagc aagcgcggtt cagcgcgacg
    22441 tggcgtgtgt agttgttccg gcaaactccc gagatacggg aagcccggtt gaaaacctaa
    22501 aaaccagacc acgtattcca cggaggagtg caattcaaca acctgttttt cgctcaaccc
    22561 acaatgcgcc gcgaccaccg ccaaatccgg tccgcctgcg ccaccgtaaa caaccggaat
    22621 ttcaataaaa cgagactccg gctccagcgc ctcactctcc tcccaccagc gttgcaaacg
    22681 ctctatggca tccagcgcca gcgactcagg attacgtaaa atcaccgtga tattgttcat
    22741 gccgggaatg gcttcaacca cattcggcat atccaccaga cgctgtgcca gtcgccagat
    22801 ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc agcactaccg ccgtttcacc
    22861 tatcagataa caacgcgctc gttgcacttt cgatccctct gattattcgt tatgcaggat
    22921 taggaatatc aataaaggtc acatcaagat cggtattttc attcagccac tcgctcaacg
    22981 cgcgaatacc accacgttca gtggcatggt gacccgcagc ataaaaatgc aatccctgct
    23041 cgcgggctga atgaatggtc tgttcagaaa cttcgccagt aataaaagca tccacgccaa
    23101 aacgcgcggc gctatcgata aaactttgcc cgccgcccgt gcaccaggca acgcgctgga
    23161 ccacctcagg tccggtatcg ccacaccata acggcttacg tcccagacgc gcttcaatcc
    23221 aggaggctag ttccagtccc ggcacaggca tggtcagttc gccccacggc accaacggct
    23281 caatttcgcc catgaccgag atccccagta acgccgccag ctgtgcgtta ttgcccagct
    23341 caggatgtgc gtcaagcggc aaatgccagc catacaggtt gatatcattt gccagcaacg
    23401 tttttaaacg gttacgcttc atgccgcgaa tgaccggaga ttcgcctttc cagaagtagc
    23461 catgatgcac aatgaccgca tccgcgccca gacgcactgc ctcatcgagc aaagcctggc
    23521 tggcagtgac accggtaaca attttttgca ccgtctcttt gccttccacc tgcaaaccat
    23581 tcggcgcgta atcactaatc gccgcgctgt tcagtttttc gttaatcagt tgttccagtt
    23641 cggtgttttt catcttcgcg ccttccgtat tctgatagct acaccgttaa catagcgtta
    23701 cctcgcctgt tcgtcgatag ccaatctttt gacatattaa gaaacaaacc cgacgccaaa
    23761 agacgcaaat tgtttatatc atcacgcttc agctaatgta tatttatgca atcagacagc
    23821 atattgcata aataatcaga tctgtatcaa agttttaact ttaaggaaac acaagcacgc
    23881 cgcgcaacta ttacgtgacg ttgcagtcaa ttgtggaatt attatgaata aacacgcatc
    23941 acagccgcgc gctatttact atgtcgttgc gctgcaaatc tgggaatatt ttagctttta
    24001 cggcatgcgt gccctgctga ttctctatct caccaatcaa ctaaaataca acgatactca
    24061 cgcctacgag ttatttagcg cctactgttc gctggtgtat gtcacgccaa tcctcggtgg
    24121 ctttttggcg gataaagttc tcggtaatcg catggcggtg atgctggggg cgttgttgat
    24181 ggcgatcggt catgtggtgc tgggtgccag tgagatccat ccgtcattcc tctatctgtc
    24241 cctggcgatt atcgtttgtg gctatggtct gtttaaatcc aacgtcagtt gcctgctcgg
    24301 cgagctgtat gagccaaccg atccgcgtcg tgatggcggt ttctcgctga tgtatgcggc
    24361 gggtaacgtg gggtctatta tcgcacctat cgcctgtggt tacgcccagg aagagtatag
    24421 ctgggcgatg ggctttggcc tggcggcggt tggcatgatc gcgggtctgg tcattttctt
    24481 atgtggcaat cgtcatttca ctcatacccg cggcgttaac aaaaaagtac tgcgtgcgac
    24541 aaactttctc ctgcccaact ggggatggct gctggttctg ctggtggcaa cgcctgcgct
    24601 gatcaccgta ctgttctgga aagagtggtc ggtatacgcc ttgattgtcg cgactattat
    24661 tggcctgggt gtattggcaa aaatttatcg caaagcagaa aaccagaaac agcgtaagga
    24721 gctggggctg attgttaccc tcaccttctt cagcatgttg ttctgggcat tcgcacaaca
    24781 ggggggtagc tcgattagcc tttatatcga ccgcttcgtt aaccgcgata tgtttggtta
    24841 taccgttccg actgctatgt tccagtcgat taatgccttc gcagttatgc tgtgcggtgt
    24901 gttcctggcg tgggtggtta aagagagcgt cgcaggtaat cgtaccgtgc gcatctgggg
    24961 aaaatttgct cttggccttg gcctgatgag cgccggattc tgcattctga ccttaagcgc
    25021 ccgctggtcc gcaatgtatg gtcactcttc tctgccacta atggtgttag gcctggcggt
    25081 gatgggcttt gcggaactgt ttatcgaccc ggttgctatg tcgcagatta cgcgcattga
    25141 aatccctggc gtgactggcg tattaactgg catttatatg ctgctttccg gcgcgattgc
    25201 gaactatctg gctggcgtga ttgccgatca gacatcgcag gcttcgtttg atacttccgg
    25261 ggcgatcaac tactccatca atgcatatat tgaagtattt gatcaaatta cctggggcgc
    25321 actggcgtgt gtaggagtgg tgttgatgat ttggctgtat caggcgctga aattcagaaa
    25381 ccgcgcgctg gcgctggagt cttaacaggc cagcaccgat gcctgataaa acgctcgctg
    25441 cgttttatca ggctcctttc ctcgcttctt cgtacgctgc cagcgttcgc agccttgcct
    25501 ctttgtgatc aactatcggt tgcggataat ccagcgtcac acctgctttc tgcgcccact
    25561 tccacggctc atgcaccact ttccctggca catcgcgcag ttccggtagc cactggcgga
    25621 taaactcgcc ttcgcggtca aattcctcgc cctgggttgt cgggttaaaa atacgaaaat
    25681 acggcgctgc atcggttccg gttgaagcgg cccactgcca gccaccgtta ttggctgcca
    25741 aatcaccatc aatcagctgc gacatgaaat atcgctcacc ttcgcgccag tcgatcaata
    25801 aatctttcac caaaaaactg gctgtaatca tccgtagccg gttatgcatc cagccagtgc
    25861 tgttaagctg acgcatggcg gcatcgacaa tcgggtatcc ggttttacct tcctgccagg
    25921 cctttaaata agtgggattg ctttgccact gtacacgatc cgtccaggca ataaatggac
    25981 gatgtttaca caacgaaggg taatacgtca tcagatgacg gtagaactcg cgccagatca
    26041 gctcattaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt tccgccaaca
    26101 ggcgatgcaa acactggcga ggcgataacc cgcccgttgc cagactggcg gacaaacgac
    26161 tggtgccttc cactgccgga aaatctcgtt gttgctcata ctctccggca ccgttctggc
    26221 aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc gcagtatcga
    26281 aagactgacg agggtaattc agcgtaattg atagcgcggg atctatcgat ccactactac
    26341 gtacttttgg cgcagcgacg cactccggca ccccttctcg caaccgtttc agccaggcgt
    26401 ttttaaatgg cgtaaagact ttgtacatct catgattgcc agtcatcacc gcccccggcg
    26461 gcaggatcac gctgtcgtca aatccttcac acaccacgtt acgcagcgtt ctctcaacct
    26521 gcacatcccg cgcccgctca ttcacttcat actgatagtt ataaaaaaga tgagtaacgc
    26581 tgttttccgc gcacacctgt ttaactattt cgacactggc ggcaaagtca tccacttcgc
    26641 ggaacaataa aggaatgcct ttttccgcca gcgctattcg tagcccgttc agttgtgcat
    26701 tgataaattc agcctgacgc ggcgacatat tatgcgccgc ccactggcgt ggtgtagcga
    26761 tatacaacgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagc gcgagattat
    26821 cgtgctgacg taaatcctgg cgaaaccaga ccagatgggt agtcataaaa ctcctgcatg
    26881 gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgctctgaaa gccaggcgtc
    26941 aggataaaac gccatatagt gtttgatacg catcagcggc gcaagaagcg gttgctcgcc
    27001 acggcgataa ctgtcgataa gcgccgccag ctgctggcgt tctgtggaat caatatgtgg
    27061 gcgaaaatat ccctgaacgt gcatcaaaac gttagtatga tcacgccggt tggcagggtg
    27121 agaaagcaaa acaattacgc gttggcggta ctggttgtaa tagtcatcca gattttgcca
    27181 ctggtgaata tcagccacaa acgggccaag tttgcggtac tccggctggg aatgggccag
    27241 aaagacgagt ttatagcggc tgtgataatc gttgagtgcc ccgcgcgtta agccatgttc
    27301 tttcagtcga tgtaactcat gaagggcgaa gatacggatt agggtgctgt catcaaatcg
    27361 ttgctgattc attactgctc ctgaacggtt tacaggagta agtgtagacg ttgggaaatg
    27421 atatttgtga agttaatcct gttgtagcag ggcaagatta cgaaagcccg ctccccgcaa
    27481 ggactgacgt caggtagttt ctgtccatgg ctgccctttc cattcaaacg tgaaatcaat
    27541 aagttcatca gggacatcgc aaacaatatg aagacggatt tcttctctgc cgtgactctt
    27601 ttcactcatt gcgtaactgt catgctctgg attatttaat tctttcagtg ggaatttttc
    27661 ctcaaaggct ttatttagtc gtccctgatt tccttttaca gcgaatacat aatcaccgcc
    27721 ctgtttttgt atcttctctg caatatcttt ctggcaaccc atcgcatcag ttgtgatgat
    27781 ttttccttta atatccaaca tgttaagaag ttcagggata gctgtgatct cattggattt
    27841 ctcatccgtc ctgatctgtc cgatgaccag actgtgcatt gttgagaacg cactaatgac
    27901 atgaatcgct cccctgcggc gactcttgtc ataagaatgc cggagcgttt ttccatcaat
    27961 tgcaataacg tctttatcat ctgaagaatg gcagtcacgc atccagttaa taaagcactc
    28021 gtgaaatttt gaaggactga tacaggatac aactctggcg atggtatcgt gaacaggaat
    28081 accattttaa aaatcaccat attgttttaa aaaatcgaga tgtgtttccc caaaatcctc
    28141 tatatcttcc caaccgtctg cacaagaaat aacggcacaa atagtcaaca gtagaatatc
    28201 cgacaattta tgttccactt tcctggcatg tctgtaatcg gaagtaatag aaatatg
//
LOCUS       DAOTRK010000057        27759 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.057, whole genome shotgun sequence.
ACCESSION   DAOTRK010000057 DAOTRK010000000
VERSION     DAOTRK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.057"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(30..1679)
                     /gene="pgi"
                     /locus_tag="QIE41_002307"
     CDS             complement(30..1679)
                     /gene="pgi"
                     /locus_tag="QIE41_002307"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDS3531187.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVNYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2204..3553
                     /gene="lysC"
                     /locus_tag="QIE41_002308"
     CDS             2204..3553
                     /gene="lysC"
                     /locus_tag="QIE41_002308"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDS3531188.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3806..4078
                     /locus_tag="QIE41_002309"
     CDS             3806..4078
                     /locus_tag="QIE41_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDS3531189.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4079..4951)
                     /gene="rluF"
                     /locus_tag="QIE41_002310"
     CDS             complement(4079..4951)
                     /gene="rluF"
                     /locus_tag="QIE41_002310"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDS3531190.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5299..6246
                     /locus_tag="QIE41_002311"
     CDS             5299..6246
                     /locus_tag="QIE41_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDS3531191.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQVQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6316..7122
                     /locus_tag="QIE41_002312"
     CDS             6316..7122
                     /locus_tag="QIE41_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS3531192.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7132..7539
                     /locus_tag="QIE41_002313"
     CDS             7132..7539
                     /locus_tag="QIE41_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDS3531193.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPDENAGDIV
                     AKLEKLVSIHNHDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQSCVVWRQVATAEEDF"
     gene            7539..8033
                     /locus_tag="QIE41_002314"
     CDS             7539..8033
                     /locus_tag="QIE41_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDS3531194.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8099..8896
                     /locus_tag="QIE41_002315"
     CDS             8099..8896
                     /locus_tag="QIE41_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDS3531195.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTQGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATTSTALDQLDD"
     gene            8907..9731
                     /locus_tag="QIE41_002316"
     CDS             8907..9731
                     /locus_tag="QIE41_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDS3531196.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9783..11012
                     /gene="sorE"
                     /locus_tag="QIE41_002317"
     CDS             9783..11012
                     /gene="sorE"
                     /locus_tag="QIE41_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDS3531197.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11394..12083
                     /gene="pepE"
                     /locus_tag="QIE41_002318"
     CDS             11394..12083
                     /gene="pepE"
                     /locus_tag="QIE41_002318"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDS3531198.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12173..13804)
                     /locus_tag="QIE41_002319"
     CDS             complement(12173..13804)
                     /locus_tag="QIE41_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDS3531199.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14024..17707)
                     /gene="metH"
                     /locus_tag="QIE41_002320"
     CDS             complement(14024..17707)
                     /gene="metH"
                     /locus_tag="QIE41_002320"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDS3531200.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            17907..18731
                     /gene="iclR"
                     /locus_tag="QIE41_002321"
     CDS             17907..18731
                     /gene="iclR"
                     /locus_tag="QIE41_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDS3531201.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            19047..21233
                     /gene="espL4"
                     /locus_tag="QIE41_002322"
     CDS             19047..21233
                     /gene="espL4"
                     /locus_tag="QIE41_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HDS3531202.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQTDKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDALDKQKLNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDE
                     IPPPLSPATMNLLMAIGQNHQLTQLMTQLQKMPELHRTEMLTAYNSINLPGLYLAINY
                     GNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSIL
                     NVLPKLAATHHLDNEQVYKFLSAKNRTSSHVLYHVMANGDADMLKIVLDALPLLIRTC
                     HLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPCLAQEINISASDIVDL
                     LTAKSLARDTGLFMAMQRGHMNVIKTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYP
                     GLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFELDH
                     RVIAELILNTINKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(21202..22938)
                     /gene="aceK"
                     /locus_tag="QIE41_002323"
     CDS             complement(21202..22938)
                     /gene="aceK"
                     /locus_tag="QIE41_002323"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDS3531203.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCAAPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(22987..24291)
                     /gene="aceA"
                     /locus_tag="QIE41_002324"
     CDS             complement(22987..24291)
                     /gene="aceA"
                     /locus_tag="QIE41_002324"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDS3531204.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(24321..25922)
                     /gene="aceB"
                     /locus_tag="QIE41_002325"
     CDS             complement(24321..25922)
                     /gene="aceB"
                     /locus_tag="QIE41_002325"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDS3531205.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMCANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(26191..27120)
                     /gene="metA"
                     /locus_tag="QIE41_002326"
     CDS             complement(26191..27120)
                     /gene="metA"
                     /locus_tag="QIE41_002326"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDS3531206.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            27277..27720
                     /locus_tag="QIE41_002327"
     CDS             27277..27720
                     /locus_tag="QIE41_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDS3531207.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRVELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         6788 a         7051 c         7085 g         6835 t
ORIGIN      
        1 acgtcggcat ttttattaag gttatttaat taaccgcgcc acgctttata gcggttaatc
       61 agaccattgg tcgagctatc gtggctgctg atttctttat catctttcag ctctggcaga
      121 atacggttcg ccagttgttt acccagctcc acgccccact ggtcgaaggt gaagatgttc
      181 agaatcacgc cctgagtaaa gattttgtgc tcatacagcg caatcaacgc acccaggctg
      241 aacggagtga tttcgcgcag caggatggag ttggtcgggc ggttaccttc gaacactttg
      301 aacggcacca cgtagtcaag cgttgccgga tctttaccct gatcacgata ttcctgctca
      361 accacttcgc gggatttacc aaacgccagc gcttcggtct gagcaaagaa gttagacagc
      421 agtttctggt gatgatcaga gagcgggtta tgggtgatag ccggggcgat gaaatcgcac
      481 ggtaccattt tggttccctg gtggatcagc tggtagaacg cgtgctgacc gttagtgcct
      541 ggttcacccc agataatcgg gccagtctgg taattcacga cgttaccgtt acggtcaacg
      601 tacttaccgt tggactccat attgccctgc tggaagtacg ccgcgaaacg gtgcatatac
      661 tggtcatacg gcagaatcgc ttcagtttcc gcgccaaaga aattgttgta ccagatgcca
      721 atcagcgcca gtagtacagg caggtttttc tcggcaggcg tggtggagaa atgcttgtcc
      781 atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa agccgatgga gagaacaatc
      841 gacaggccaa tcgctgacca taaagagtaa cgaccaccga cccagtccca gaactcgaac
      901 atgttggcgg tatcaatacc aaactcgcca acggctttgg cgttggttga gagcgccgca
      961 aagtgtttcg caacgtgttt ttcatcacca gccgctttca ggaaccagtc acgcgcacta
     1021 tgggcgttgg tcatggtttc ctgagtggtg aaggttttag atgctaccag gaacagcgtg
     1081 gtttccgggt ttactttttt cagcacttcc gcgatgtgag tcccatcgac gttagaaaca
     1141 aagtgcatgt tcaggtggtt tttgtacgga cgcagtgctt cggtcaccat gtatgggccg
     1201 aggtcagaac cgccgatccc gatgttcact acgtcagtga ttgctttgcc ggtataaccc
     1261 ttccactcac cggaaataat cgcttgcgag aaggttttca tcttctccag caccgcgttg
     1321 acttccggca taacgtcttt gccatcaacc agaatcgggg tattgctacg gttacgcagc
     1381 gctacgtgca gcacggcgcg gttttcggtg cggttgatct tctcaccaga gaacatcgac
     1441 ttaatcgcgc ccgccagatc gcactctttc gccagatctt gtaatttcgc cagcgtctct
     1501 tcagtgatgc ggtttttgga gtaatccacc agcatctgat cgtcgaaggt tgcggagaac
     1561 ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc tttcatttca
     1621 tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg ttggattgat gtttttcatt
     1681 agcaatactc ttctgatttt gagaattgtg actttggaag attgtagcgc cagtcacaga
     1741 aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt agcgcagtga
     1801 agcatttatt agctgaacta ctgaccgcca ggagtggatg aaaaatccgc atgaccccat
     1861 cgttgacaac cgccccgctc accctttatt tataaatgta ctacctgcgc tagcgcaggc
     1921 cagaagaggc gcgttgccca agtaacggtg ttggaggagc cagtcctgtg ataacacctg
     1981 agggggtgca tcgccgaggt gattgaacgg ctggccacgt tcatcatcgg ctacaggggc
     2041 tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg cgtttcgcaa gtggtggagc
     2101 acttctgggt gaaaatagta gcgaagtatc gctctgcgcc cacccgtctt ccgctcttcc
     2161 cttgtgccaa ggctgaaaat ggatcccctg acacgaggta gttatgtctg aaattgttgt
     2221 ctccaaattt ggcggtacca gcgtagctga ttttgacgcc atgaaccgca gcgctgatat
     2281 tgtgctttct gatgccaacg tgcgtttagt agtcctctcg gcttctgctg gtatcactaa
     2341 tctgctggtc gctttagcgg aagggctgga acctggcgag cgattcgaaa aactcgacgc
     2401 tattcgcaac atccagtttg ccattctgga acgtctgcgt tacccgaacg ttatccgtga
     2461 agagattgaa cgtctgctgg agaacattac tgttctggca gaagcggcgg cgctggcaac
     2521 gtctccggcg ctgacagatg agctggtcag ccacggcgag ctgatgtcga ccctgctgtt
     2581 tgtcgaaatc ctgcgcgaac gcgatgttca ggcacagtgg tttgatgtac gtaaagtgat
     2641 gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta gccgcgctgg cggaactggc
     2701 cgcgctgcag ctgctcccac gcctcaatga cggcttagtg atcacccagg gatttatcgg
     2761 tagcgaaaat aaaggtcgta caacgacgct tggccgtgga ggcagcgatt atacggcagc
     2821 cttgctggcg gaggctttac acgcatctcg tgttgatatc tggaccgacg tcccgggcat
     2881 ctacaccacc gatccacgcg tagtttccgc agcaaaacgc attgatgaaa tcgcgtttgc
     2941 cgaagcggca gagatggcaa cttttggtgc aaaagtactg catccggcaa cgttgctacc
     3001 cgcagtacgc agcgatatcc cggtctttgt cggctccagc aaagacccac gcgcaggtgg
     3061 tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc cgcgcgctgg cgcttcgtcg
     3121 caatcagact ctgctcactt tgcacagcct gaatatgctg cattctcgcg gcttcctcgc
     3181 ggaagttttc ggcatcctcg cgcggcataa tatttcggta gacttaatca ccacgtcaga
     3241 agtgagcgtg gcattaaccc ttgataccac cggttcaacc tccactggcg atacgttgct
     3301 gacgcaatct ctgctgatgg agctttccgc actgtgccgg gtggaagtag aagaaggtct
     3361 ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc tgcggcgttg gcaaagaggt
     3421 attcggcgta ctggaaccgt tcaacattcg catgatttgt tacggcgcat ccagccataa
     3481 cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg gtgcaaaaac tgcatagtaa
     3541 tttgtttgag taaatactgt atggcctgga agctatattt cgggccgtat tgattttctt
     3601 gtcactatgc tcatcaataa acgagcctgt actctgttaa ccaacatctt tatcggagaa
     3661 taattgcctt taattttttt atctgcatct ctaattaatt atcgaaagag ataaatagtt
     3721 aagagaaggc aaaatgaata ttatcagttc tgctcgcaaa cgaattcccg tacactgaac
     3781 tctctggctc actgagggca ccagcatggc actcccccgt attacccaaa aagagatgac
     3841 tgaacgcgag cagcgtgaac ttaaaacact gctggatcgg gcgcggattg cgcatggtcg
     3901 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
     3961 cgagcgtgag gctgaagcga aaaaagcccg ccagttaaag aaaaagcagg cttataaacc
     4021 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctgatt
     4081 agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac gccgggcgac
     4141 cgcctttttc cgccgttttt tccatcttaa cgactggacg tttaatgcct acagttttcg
     4201 gtttggcctt cgctttaggt tttgcctcgg aagaggaatt ttcaatgagc ttaaagaggt
     4261 cgattaactc atcgtcagtt aaatcacgcc attcccccag cggaatgccg cttaaactaa
     4321 cgttcatgat gcgcgtacgt tccagctttt tcacttcgta accaaagtgc tcgcacatgc
     4381 gacggatctg acggttcagc ccctgcacca gggtaatgcg gaagacaaac ggcgcttctt
     4441 ttttgacttt acactttttc gtcaccgtcc cgaggatcgg cactcccgcc cccataccac
     4501 gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc tcatgatcgt
     4561 tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa atcagcccct
     4621 gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta acgaaatcga
     4681 caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc ttgttcaggg
     4741 cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt actttcacaa
     4801 cgtcgccggg tttcacctga tcgccaatgg tggctcgctt gccattaagg aacacattgc
     4861 cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg ctgatgtatt
     4921 tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc ggaatatacc
     4981 gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa tgtgatctgc
     5041 cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct ggcaaaataa
     5101 ggataatcaa catattacga accgctgcac acctgtgctg atgactacta cgcagagaaa
     5161 caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat cgctttgaaa
     5221 ggccatcatg atggggttat atcagatcaa gcggtgagaa agcgtatttg cacaaatgtg
     5281 cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt gaagattgcc
     5341 caactctatt acgaacagga tatgacgcag gtgcaaatcg cgcgcgaact gggtatttac
     5401 cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt caccatcgcc
     5461 atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca aaagtttggc
     5521 ctgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca actggcgatg
     5581 atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga tattgtcggt
     5641 ttttcctggg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaggc ggggcaatcg
     5701 cggcagttaa tctgcgtacc aattattggc ggaccgtccg gtaaactcga aagccgctat
     5761 cacgtaaaca cattaaccta cagcgcggca gcgaagctga aaggggaatc gcatctcgca
     5821 gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca gtctcagcac
     5881 tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg aattggctca
     5941 ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga gagtgacgac
     6001 ctgaatgccc gccaggttgc tggcgatatt tgctcgcgct tttttgatat tcacggcgca
     6061 atggttgaaa cgaatatgag cgaaaaaaca ctctctatcg aaatgaataa attaaagcag
     6121 gcacgatatt ccattggcat tgccatgagt gaagaaaaat acagcggaat tgttggtgca
     6181 ctgcgtggaa aatatattaa ttgtctggta acgaatagca gcacagctga actattactg
     6241 aaataaaaat cttcccgatg tttaccgtaa taaaaattac ggcgggtccc tattatctaa
     6301 aaacaggagt ctgttatgca aacgtggtta aatttgcagg ataaaatcat tatcgtcacc
     6361 ggcggcgcat ccggtatagg tctggcgatt gtagaggaat tattagcaca aggcgcgaat
     6421 gtacagatgg tcgatattca cggtggcgat ggacaatatg aaggtcataa aggctatcag
     6481 ttttggccga ccgatatttc cagcgccaaa gaggtaaatc atacggtagc agaaattatc
     6541 cagcgttttg gtcgcatcga cggtctggtt aataacgccg gggtcaattt cccacgtctg
     6601 ctggtcgatg agaaagcgcc tgccgggcag tatgaactca acgaagctgc attcgaaaaa
     6661 atggtcaata tcaaccagaa aggcgttttt ctgatgtcgc aggcggtggc gcgacagatg
     6721 gtcaaacaac acgatggcgt gattgtgaat gtttcctcag aaagtgggct ggaaggctca
     6781 gaaggccaga gctgctacgc cgccactaaa gctgcgctca atagcttcac tcgttcctgg
     6841 tcgaaagagc tgggtaagca cggtatccgt gtggtcggta tcgcgccggg gattctggaa
     6901 aaaacaggac tgcgcacgcc ggaatatgaa gaagcgctgg cgtggacgcg caatatcacc
     6961 gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc ctattgggcg cgccggaaga
     7021 ttagcagaag tcgctgattt tgtttgttat ctgctgtctg aacgcgccag ctacatcacc
     7081 ggagtaacca ctaacattgc gggcggcaaa acgcgcggat aaggaggctt tatggtcaac
     7141 gccatttttt gtgcccacgg caaattagcc tgcgccatgc tggaatcggt tcagatggtt
     7201 tacggcaacg ccaatgttga agccgttgaa tttgtacccg acgagaacgc cggagacatt
     7261 gtggcaaagc tggagaagtt agtgagcatt cacaatcatg acgagtggct aatcgccgtg
     7321 gatttacagt gcggcagccc gtggaatgcg gcagcgatgc ttgcgatgcg caatcctcgc
     7381 ctgcgggtaa tcagcggcct ttctctgccg ctggcgctcg aactggttga taaccaggac
     7441 agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa tcgcaaagca atcctgcgtc
     7501 gtctggagac aggtggcaac ggcagaggag gatttctgat gaacattacg ctcgcccgca
     7561 ttgatgaccg cctgatccac ggccaggtca ctaccgtctg gtctaaagtg gctaacgccc
     7621 agcgcattat tatctgcaat gacgaagttt ataacgatga agttcgccgg acattattgc
     7681 gtcaggctgc gccaccggga atgaaggtta acgtggtcaa tattgaaaaa gccgtcgcgg
     7741 tttatcacaa cccgcaatat caggacgaaa ccgtttttta tcttttcact cgcccacagg
     7801 atgccttagc gatggttcgc cagggggtaa aaataggcac cctgaatatt ggcggcatgg
     7861 cctggcggcc cggtaaaaaa cagttaacca aagcggtttc attagatgat gatgatatta
     7921 acgcgtttca tgagttaaat aatcttggcg tcatactgga tttacgcgtc gtagcgtcag
     7981 atccctcaat caatattatc gacaaaataa acgaacagtt aatcgcaaat taaataatag
     8041 cgcctgtgta catatgcttt aacaggcagg gatgacgaca cttaaaaggt gacaaatcat
     8101 ggaaataagt accctacaaa tcattgctat atttcttttt tcctgtattg ccggaatggg
     8161 cagcgtgctg gatgaatttc agactcatcg cccattaatt gcctgtacgg tgattggttt
     8221 aattctcggt gatttaaaaa ccggaataat actcggtggt acgctggaat tgatagctct
     8281 cggctggatg aacgtcggcg cggcgcaatc tccggattct gcactcgcca gcataatctc
     8341 cgccattctg gttatcgttg gtcagcaaag catcgccacc ggaatcgcca tcgcgttgcc
     8401 tgtggctgcg gcaggccagg tgctgacggt gtttgcccgt accatcaccg tagtgttcca
     8461 gcacgcggcg gataaagcag cggaagaggc gcggtttcgc actctcgata ttctgcatgt
     8521 ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg gcactgattg tctcgctgtt
     8581 cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt cccgaatttg tgacccaagg
     8641 actacagatt gctggcggtt ttatcgtggt ggtcggttac gccatggtgc tacgtatgat
     8701 gggtgtgaaa tatttgatgc ctttcttttt cctcggtttc ctcgcaggtg gctacctcga
     8761 tctcagtctg ctggcgttcg gtggcgtcgg cgtgatcatg gccctgctct acatccagct
     8821 aaatccacag tggcgtaaag ctgaacctca actccaggcc accacctcca ccgcccttga
     8881 ccaacttgac gattaacgga gccatcatgg aacagagaaa aattacacgc agcgatctgg
     8941 taagcatgtt tctgcgctcc aacctgcaac aggcgtcctt taactttgaa cgtattcacg
     9001 ggctgggttt ttgctacgac atgatccccg ccatcaagcg actttaccca ttaaaagagg
     9061 atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa taccacgcca gccgtatgtg
     9121 gcccggtcat cggcgtcact gccgccatgg aagaggcgcg ggccaacggc gcggaaattg
     9181 atgacggtac cattaacggc atcaaagtcg gtctgatggg accattggca ggagttggcg
     9241 atccactggt ctggggaacg ctgcgcccga ttaccgccgc gctcggcgca tctctggcac
     9301 tttcgggcaa cattctcggc ccgctgctgt ttttctttat tttcaacgcg gtgcgtctgg
     9361 cgatgaagtg gtatggccta caactcggct ttcgcaaagg ggtgaatatc gtcagcgata
     9421 tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc gattctcgga ctgtttgtga
     9481 tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc gttggtggtt tcacaaacgc
     9541 atgccgccga tggctccacc gtcaccatga ccgtgcagaa cattctcgac caactttgcc
     9601 ctggtttgct ggcgctcggt ctgacgctac tgatggttcg tctgctcaac aaaaaaatta
     9661 acccggtatg gctgattttc gccctgtttg gcttagggat tatcggcaat gcgctgggct
     9721 tcctgtcctg attcttcgcc ccggcacgac tgccggggcc atcgctcaac atgaggtggt
     9781 ttatgaaaac aacagctctg cgtctttatg gtaaacgtga tttacgcctg gaaacctttg
     9841 atcttcctga aatgcaggag gatgaaatcc tcgcgacggt ggtcaccgac agcctgtgcc
     9901 tctcttcctg gaaagaggcc aatctgggtg aaaaccataa aaaagtaccc gacgatgtgg
     9961 cgaccaaccc catcatcatc ggccacgagt tttgcggcga tattctggcc gtgggtaaaa
    10021 agtggcagca caaattccag ccgggtcagc gttatgtgat tcaggccaac ctgcaactcc
    10081 ccgaccgccc ggactgcccc ggctactcct tcccgtgggt aggcggcgaa gccacgcatg
    10141 tggttattcc caacgaggtc atggaacaag attgcctgct ggcatacgac ggcgaaacct
    10201 attttgaagg ctcgctggtt gaaccgcttt cctgcgtgat tggcgcgttc aacgccaact
    10261 atcatcttca ggaaggtagt tataaccaca cgatggggat tcgcccgcaa gggcgcacgc
    10321 tgatcctcgg cggcaccgga ccaatgggac tgttggcgat tgattatgcg ctacatggac
    10381 ccgttaaccc gtcgctgctc gtcattaccg ataccgacaa cgacaaattg agttatgcgc
    10441 gcaagcacta tccatcagaa ccgcaaacac tgattcatta tctcaatgcc gccgatgcag
    10501 catttgatac gttaatggca ctgagtggcg gtcacggctt cgatgatatt ttcgtctttg
    10561 tgcctaatga aggactggtg actctcgcct cttccttgct ggcgacagat ggttgcctga
    10621 atttcttcgc cggaccgcag gataaacatt tcagcgcgcc aattaatttc tacgatgtgc
    10681 attatgcatt tacccactac gtgggcacgt caggcggcaa taccgatgac atgcgcgcag
    10741 cagtcaaatt gattgaagag aaaaaagtgc aggccgcaaa agtggtaaca catattcttg
    10801 ggctgaatgc cgcgggcgaa accacgcttg aattgcctgc cgtcggcggc ggcaaaaagc
    10861 tggtgtatac cgggaaatac ctgccgctta tgtcactcac gcagattcag gatcaagcac
    10921 tggcggcaat tctggcgcgt catcagggga tctggtccgg tgaggcggag caatatctgc
    10981 tcactcatgc agaggcaatt tcccatgatt aatcgcgcta cttaactctg catgtcgctg
    11041 gctggtcgcc gcggcaactt cgcgaccgga tgtgcggctg attgctgaag cagcacaatt
    11101 tgcatgagaa ggataaaaaa tatcagcgcc ttaaggggcg ctcaaattgg tggcaaagta
    11161 cacattgttt atgccggatg cggaatgaag ccttattcgg catacataat ctcgaaaatt
    11221 caatatattg cagttatctg gtaggactgg taagcgtagc gcatcaggca atgctgtgtt
    11281 tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt gagccatgtc agtaagtggt
    11341 taaattcgca gtggtagcat gcctcccgtg atttcttact aaccggagaa gtaatggaac
    11401 tgcttttatt gagtaactcg acgctgccgg gtaaagcctg gctggaacat gcactgccgc
    11461 taattgctga acagttgcag ggtcgccgtt cagcggtgtt tatccctttc gctggcgtaa
    11521 cgcagacctg ggatgattac acagcgaaaa cggctgcggt tctcgctccg ctgggtgttt
    11581 ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc gattgaaaat gctgagatcg
    11641 tgattgtcgg cggcgggaat actttccagt tgctgaaaca gtgccgcgag cgcgggctgc
    11701 tggcaccaat tactgacgtg gttaaacgtg gcgcgctgta tattggctgg agcgcaggcg
    11761 ctaaccttgc ttgcccaacc attcgtacca ccaacgatat gccgattgtc gatccacaag
    11821 gtttcgatgc gctgaatctg ttcccgttgc aaatcaaccc gcacttcacc aacgcgctgc
    11881 cggaaggcca taaaggtgaa acccgtgaac agcgtattcg cgaactgctg gtcgtcgcgc
    11941 cagaactcac aattattggt ctaccggaag gtaactggat cacagtgagt aaaggtcacg
    12001 caacgctggg tggcccgaac accacttatg tgtttaaggc tggtgaagaa gcggtgccgc
    12061 tggaagcggg tcaccgtttt taagtgaatt gccggatgca tgatccggca tcttgtacat
    12121 agtgcctgat gcgacgcttt acgcgtctta tcaggccttc aggggcgcag tattaatact
    12181 cgtcccgtcc ttcatcttca tcaggctgtt ccagcacact gtaagcgact gaacaaaaca
    12241 gcgagttcag gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc
    12301 tctgcacgtt ttgctgatgc aggcgatcga cgtgagcgtg ggaatagcgg cgattaagaa
    12361 tgcgaaaacg atgtttgctg cggcgcaaac gacgggcgct ggtaacatcg ccagagaaga
    12421 aaaccgacat tgccagtttt aaattactga gcaattgctc atagagcgca tccagttcct
    12481 tcaagccatc aagcgaaaat gctcgccttg cagccagcga tttgtcggca atttcactac
    12541 ccatgcgctc gacgatatcg gaggcttgtt caaggtttag cgacatttcg atgatctccg
    12601 cccagcggcg cgactcttct tctgccagtt cctcttttgg cattcgcgcc agataaagtt
    12661 taatcgcggt atagagcacg ttgatatcat ccgccagctt gcgcagctct ttctcctgcc
    12721 gtggctcgcc gtgcatcact ttattcaacc cttccatcat ctgttccatg gcgtcgccaa
    12781 tgcgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga
    12841 catccagatg tttaggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt
    12901 tgcaaaaccg tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt
    12961 agaagacgtg gaaatagatc accagttccg cttttggcaa tgacaacttc cccattgtct
    13021 ctgccagcaa atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac
    13081 tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca
    13141 ggccggaacc gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc
    13201 ctgcagcggt cagagtcgcg gtcagcagta cagcagcaag gctggagtag ctgataatgg
    13261 cgaacatcgc gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca
    13321 cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccaacgcca
    13381 gcaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc
    13441 gtccgaggaa aaaaatcacg ccgataaaaa taagtaatgg tgacagccag gataaatcga
    13501 aggtgagaat acgcgccatt agcgccgtcc cgacatctgc acccagcaca atcaccagag
    13561 ccggtgcgag ggctaccaga tcctgagcga cgaacgaggt caccagcatg gtggtggcat
    13621 tactgctctg taccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt
    13681 cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc
    13741 gaacaatatg agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta
    13801 acacgagttc aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga
    13861 ccggaaacac gctgactggc atctttactg tgccttttga ccttcgtttt tccggctaag
    13921 attattccag cctgattcca gtataagtgt ttcatattaa taaagagaca gggcactcat
    13981 tgagcgccct gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc
    14041 ccagattcgg tgccagccag cgctcaacat cagagacgct cataccttta cggcgggcat
    14101 agtcttcaac ctgatcgcgc tggatttgcg ccaccgcgta atacttgctg tccgggtggc
    14161 tgaagtacca acccgaaacc gatgcaccag gccacatggc gaaagattct gtgagtttca
    14221 tgccagtgtg tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg
    14281 ggcaggccgg atagcccggt gccggacgga tgccctggta gttttcgcgg atcagctctt
    14341 cgttgctgag gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga
    14401 gatactccgc aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt
    14461 aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca
    14521 cggcaaatgc gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga
    14581 gacagtagtt agcgaagccg gttttttcgg tctgttgacg cagatggtgg ctgacgttga
    14641 tcacatgggt acgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg
    14701 ggaacaggcc caccacgcca cgcggattca gcgttttttc ggcgcttaat ttatccagca
    14761 tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa
    14821 tgcgcggata cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat
    14881 tacgcagcgt ttcgatgctg gcttcgactt cctgcacgcc gagacggtgc gccaccggcg
    14941 gcgtataagc ctgccagtca aaagcgaagt cgttatcacg cgccgcttcc agcgtgaccg
    15001 gtggtgtgcg cggtttcttg cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg
    15061 tacgagcgac aaaatcatca cgttgagtat cggaaagcag cgccgccacc acgccaacgg
    15121 tgcgcgatgc gttctgtaca tacaccgtcg ggccgctgta gttctgctca attttcaccg
    15181 ccgtgtgcgc ttttgaggtg gtcgcaccgc caatcagtaa cggaatggtg aagccctgac
    15241 gctccatctc tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa
    15301 ggccaatcaa atcagcattc acttctttag cggtacggag aattttttcc gcaggcacca
    15361 tcacacccag atcgacaatt tcgtagttgt tacattgcag caccacgcca acgatatttt
    15421 taccgatgtc gtggacatcg cctttcacgg tggcgatcac catcttgccg ttggttttac
    15481 cctgctcttt gctggcttca ataaacggtt cgagataggc caccgcctgt ttcatcacgc
    15541 gcgccgattt gaccacctgt ggcaggaaca ttttgccttc gccgaacaga tcgccgacca
    15601 cgttcatgcc gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc
    15661 gggcttcttc ggtatcctgc tcgataaact cggtaatgcc tttgaccagc gagtattcca
    15721 gacgtttatt cacttcccac gagcgccact ccgcctgctg ggcgttggcg gtgtcgtcgg
    15781 ttttgctgcc gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcgcgac
    15841 ggttaagaat cacatcttcc accgcgtcgc gcagttcagc gggcaagtcg tcgtaaatcg
    15901 ccagctgccc ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga
    15961 acaccgcgtg aatggcttcg cgcaccggat cgttaccacg gaatgagaaa gaaacgttag
    16021 atacgccgcc ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttca catgcgccga
    16081 taaagtcctg cgcatagttg ttgtgctctt caatgccggt ggcgaccgcg aagatgttcg
    16141 ggtcgaagat gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc
    16201 gacggcaaat ctcgattttc cgtgcgcgag tatcggcctg tccctgttcg tcgaaggcca
    16261 ttaccaccac tgccgcacca tagcgacgca acagcttcgc gtgatggata aaggcgtcga
    16321 cgccttcttt catcgaaata gagttaacaa tgcctttgcc ctggatacac ttcagacctt
    16381 tttcaatgac atcccatttt gaggaatcga tcataatcgg cacgcgagcg atatccggtt
    16441 caccggcaat cagattgaga aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat
    16501 ccatattgat atcgataatc tgcgcgccgt tttccacctg ctggcgggcc acatccagtg
    16561 cctcgctgta tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg
    16621 tgcgttcacc cacgttcaca aacaggctat cttcgccaat gttcagcggc tccaggccgg
    16681 acaaacggca ggctacgggg atttccggca gtttgcgcgg cgctaatcct tctactgcac
    16741 gactcatcgc ggcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa
    16801 agcccgcttg cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact
    16861 caccaaaggc gttgggtagc ccggcgttcg ggtgcgctgt gacgtagcat tccgcaatcc
    16921 gtgataattc ctgcacgtac tggcgcagtt cgtctggccc cagcgcacag ttaaggccga
    16981 aagtcagcgc ttcggcgtgg cgcagagagt tataaaacgc ttcggtggtc tgcccggaga
    17041 gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca
    17101 gcgcttcaaa ctccgttttc accgcaaaga ccgccgcttt ggcgttaagg gtgtcgaaaa
    17161 cggtttcaat caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat
    17221 aggccgccac cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccggag
    17281 aaatagacgc cgtgcggttg gtcgggccga gaacaccggc aacgtagcgc ggtttctccg
    17341 gcgtgcgcgc ggtccactca tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga
    17401 tttccgccga cagggattcc atctggtaat ccgccatcgc aatggttgtg gagttgaagg
    17461 tgttggtttc gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca
    17521 cttccggttt actgagtacc agcaggtcgt tgttgccttt gaggtcgcat ggccagtcgg
    17581 caaagcgttc accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca
    17641 taccgccgtc cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc
    17701 tgctcacact tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac
    17761 tggcataaac gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt
    17821 aactcatcgg atcagttcag caactattgc attagctaac aataaaaatg aaaatgattt
    17881 ccacgataca gaaaaaggag actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa
    17941 aacccgccgt tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga
    18001 aattactgga gtggattgcc gaatccaatg gcagtgtggc actcacggag ctggcgcaac
    18061 aagccgggtt acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggct
    18121 ttgtgcgtca ggttggcgaa cttggacact gggcgatcgg cgcgcatgct tttatggtcg
    18181 gtagcagctt tcttcagagt cgtaatctgt tagccattgt ccatcctatt ctgcgcaatc
    18241 tgatggaaga gtccggcgaa accgtgaata tggcggtgct ggatcaaagc gatcatgaag
    18301 cgattattat cgatcaggta caatgtactc acctgatgcg tatgtccgcg cctatcggcg
    18361 gtaaattacc gatgcatgct tccggtgcgg gtaaagcctt tttagcccaa ttaagtgaag
    18421 agcaggtgac gaagttactg catcgcaaag gcttacatgc ctatacccac gcgacgctgg
    18481 tgtctcctgt gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg
    18541 atgatgaaga acatgccctg ggactgcgtt gtctcgcggc atgtattttc gatgagcacc
    18601 gagaaccgtt tgccgcaatt tcaatttccg gaccgatttc acgtattacc gatgaccgcg
    18661 tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg
    18721 gaatgcgctg actttttctg gcgggcagag gcaatattct gcccgtcata cccgactggc
    18781 aatagaataa ggggtctgtt aatcgcattg acgccaaaat aacttattgt catacacttc
    18841 acacgctttt attacctcag cattattttt aaacatcaaa ccacttaatt attaacaaaa
    18901 tcgcaagatc tcattccacc gaaactttta cggctgcatt atgagattga gcgatttatg
    18961 atatttcttt acttgtaatc atattaaatt ttttcactgt tctgatagtt aaaattcaag
    19021 acatcaataa acaatgagat atttaaatga ttactcgtat tcctcgtagt tctttctctg
    19081 caaatattaa taatacagcc cagacaaatg aacaccaaac cctgagtgaa ttgttttata
    19141 aagaactcga ggataaattt tctggcaagg agctggcgac gcctctatta aaaagcttct
    19201 cagagaactg tagacaaaat ggtcgtcata tctttagtaa caaggatttt gtcattaagt
    19261 tttccacgtc cgtcttacag actgataaaa aagaaattac gataattaat aaaaatgaaa
    19321 acacgacact cactcaaacc attgctccaa tatttgaaga atacctgatg gaaattttac
    19381 ctcaacgctc agacgctctt gataaacaaa aattaaatct aaaatcagat agaaaagaaa
    19441 aagaatttcc aagaattaaa cttaatggtc aatgttattt tccggggcga ccccaaaacc
    19501 gtatagtatg ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt
    19561 acaaacccca tcaagatgat tactcttcag ctgaaaaatt tctcactcac ttcaacaaaa
    19621 aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc tgaggggcgt tgcgttgctg
    19681 cctgcggtga tttcgggcta gttatgaaag catattttga caagatggaa tcaaatggca
    19741 tcagtgttat ggcagccata ttattggtgg ataaccatgc tttgacggtc cggctaagaa
    19801 taaagaacac aactgaagga tgtacccatt acgtggtttc ggtttatgat ccaaatgtaa
    19861 ctaacgataa aataagaatc atgagcgaaa gtaaagagga tattaaacac tattcactga
    19921 tggactttat gaatgtagat tatagcctcc tgaaatggtc aaatgatcat gttattaacc
    19981 aatctgttgc aataattcca gcacttccga aagaacagct attgatgcta aaaggatctg
    20041 tggatgaaat accccctcca ttatcacctg caacgatgaa tttgctaatg gcaattggtc
    20101 agaatcatca acttacgcaa ctgatgactc aactccagaa aatgccagaa ctacatagaa
    20161 cagaaatgtt gactgcctat aatagtatta acctccccgg tttatatttg gctataaatt
    20221 atggtaatgc ggatatcgtt gagactattt tcaattcatt gtcagagcca ggatatgaag
    20281 ggttactctc gaaaaaaaat ctcatgcata ttctggaggc aaaagataaa aatggttttt
    20341 ctggattatt tttagcgata tcacgtaagg ataaaaatgt tgtaacctcg attctgaacg
    20401 tcttacctaa actggccgca acacatcatt tagataatga acaggtgtat aaattcctga
    20461 gtgccaaaaa tagaacgtcc agccatgttt tataccatgt tatggcgaat ggtgatgccg
    20521 acatgctgaa aattgttttg gacgcgttac ctttgttaat tcgcacatgt catttgacta
    20581 aagaacaggt actcgatctc ctgaaggcaa aggattttta tggttgccca ggactatacc
    20641 tggcgatgca aaatggacat agcgatatcg tgaaagttat tctcgaagca ttaccctgtc
    20701 tggcccagga aattaacatt tcagcttccg atattgtaga tcttctgacc gctaaaagtc
    20761 ttgcgcgcga cacgggtttg tttatggcca tgcagcgcgg acatatgaac gttattaaga
    20821 ctatttttaa cgcattaccc actctgttta atacgtttaa attcgataaa aaaaatatga
    20881 agcccctcct cctggcaaat aattctaatg aatatcccgg tttgttttca gcgatacagc
    20941 ataaacaaca aaacgttgta gagacggttt atcttgcttt atctgaccat gcacgcctgt
    21001 ttggatttac tgctgaagat attatggatt tttggcaaca taaagcgcca caaaaatact
    21061 ctgcctttga gttggctttt gaattggatc accgggttat tgctgaatta atccttaata
    21121 cgataaataa gatggctgaa agctttggct ttacggataa ccctcgatac attgcggaga
    21181 aaaattatat ggaagcttta ctcaaaaaag catctcccca taccgtacgc taaatctttg
    21241 cctgcgccga tacgcataaa catcttccac atgtccttca cgaatacggt tttgtagtgc
    21301 gcgccagtaa tcagcgcgga acaggtcggc gtgcatctct tcaaagagtg gcccaatgcg
    21361 gggggcggcg catagccagt ggcgaaactc ttccgggaaa acatcgcccg gcgagacgct
    21421 gtaccacggt tcgctggcaa gttcgtcctc cgggtagcgc ggcggcggga tgtcgcggaa
    21481 gttcacttcc gtcatgtagc aaatttcgtc gtaatcataa aacaccacac gcccgtgacg
    21541 ggtgacaccg aagtttttaa acagcatgtc gccagggaaa atgttagcag cggcaagctg
    21601 gcgaatagcg ttaccgtatt cttcaatggc atcgcgcaac tgctgacctt ccacttgttc
    21661 cagccagata ttgagcggca ccatccgccg ctcaatataa agatggcgaa tcacaatttg
    21721 ttcgccgaga tcggtgattt tttccgctgc ttcctggagc agtaattcca ttaatgccgg
    21781 ggaaatatgc cgcttctcca gcacaaagtt ttcaaactcc tgggtgtccg ccattcggcc
    21841 cacgcgatcg tgctctttca ccaattgata gcaggcacga acgtgagcgg cagacatctc
    21901 tttctgcggc gcgaacttgt ctttgatgac tttgaatacc cgatcaaagc ccggcagcgt
    21961 aaacaccagc atcaccatgc cacgaatacc cggcgcttca atgaactgct cattacagcc
    22021 ctgtagataa acgagatatt cgcgatagct ttcggttttg gcgtgcttct ggcagccgat
    22081 agccatatac aattcagcgg tggttttacc tggcagaatt tcccgtagcc actcgaccag
    22141 cgctgcgggc agcggtgcat aaaccataaa ataagaacgc gcaaagccaa agacaatgct
    22201 cgcttcggcg gtcgtcgtca gacaggtatc aataaacagt tcgccgtcgt ccgtctggtg
    22261 gatcggcagc aaaaatggca atgtgccgga aggcgtgatc agtttgccca ccagccaggc
    22321 ggctttattg cggtaaaaca gttcgttcgc cacctgtaaa tgactttccg cgaggttgtc
    22381 tgtccccagc gtttccgtca gatgacgaac gatgtaatga atgtcacggc ttttattctg
    22441 ccagcgcaga cgcaacggca ggtcgctgat aacgcgcatc agcagagatt cccagccgtg
    22501 atcggggtga aagtctttcg ccagcgggcg tggaatggta cgaaagcggc gctctggctg
    22561 ggagctaaag ataaaaagcc gctcgggagt aagcgagcgg tggtcaaata accgacagta
    22621 cacggagtta aaaaaactct ccgcaatctc gaagcgcggg taatccggca acagccgggt
    22681 gtaatgctct ttgacgcgca gtaaaaatgc cgcgtccgtg ctttggccgt tggtaatgca
    22741 gcgcagttgc tccaccacca gacccacgtg atgatcgtaa agatggatac ggtttttcat
    22801 cgcctgctgg acggcgtgcc agtcggcttg ttcgaaacgc tgctgcgccc cggaagtcac
    22861 ttcgaggaat cgaccatact gagcgtcgaa accttgcaaa atggtttgtg caatcagtaa
    22921 ttccaggcca cgcggcatcg cctctcctta attcgcccct cgccttacgg aaaggggcgt
    22981 tggttgttag aactgcgatt cttcggtgga gccggtcagc gcggtgactg aagacgtgcc
    23041 gccctgaata atggtcgtca ctttatcgaa ataacctgta cccacttcct gctggtgaga
    23101 tacgaaggta tagccatctt tcgcagcggc aaattccggc tgctgcactt tctcaacgta
    23161 atgcttcatg ccttcgccct gggcataggc atttgccagg tcgaacatgt taaaccacat
    23221 gctgtggatg cctgccaggg tgatgaactg gaacttgtag cccatatccg acagctgctg
    23281 ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg acggcgagca
    23341 gttataagcc agcagtttgc ccggatattt cgcgtggatg gcttgtgcaa agcgacgcgc
    23401 cagttccaga tccggcgtgg aagtttcaca ccataccagg tcagcatatg gcgcatacgc
    23461 caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaaac cttcgctggt
    23521 acgctcgccg gtaataaatt cgctgtcata tgggtcgcaa tcggaggtaa tcagatccgc
    23581 cgcatcggca tcggtacgag caaccagcag ggttggaacg cctgtcacgt cagctgccag
    23641 acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt tgccgcccat
    23701 gtgaccgcat ttcttcactg atgccagctg atcttcgaag tgaactgccg ctgcaccggc
    23761 ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc
    23821 atcagcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcccgc
    23881 ggaccattgg atctgatccg cacggcggaa agtattgttg atccgctcca ccacagctgg
    23941 caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc
    24001 cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag
    24061 cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg actcgccgtg
    24121 cagcagacgc cacattttcg ccgcgcccag ttgcgccagc gtgcattcag gattgactga
    24181 accgcgtaat ttcaccacat cttccgcgct gtatgggcga gtaatgcctt cccaacgcgg
    24241 ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca tgtgcagatg
    24301 ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt caggaaatct
    24361 attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc aaaacgcccc
    24421 tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc gcccagcatc
    24481 tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg atgatggatc
    24541 cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat caggccataa
    24601 atcggcacac agccgttgcc agagatccat gcttcgatgt actgcacagc cacgcgaatg
    24661 ttggcgcaca taccttcttc ggtgcgttca ccatcacaag gtgccagcag ctgatcggca
    24721 gtaatcggcg cgtcttgttc gcgcatcact tcaagctgat ttttacggga gccgagaatg
    24781 tcgttgaata ccgccattgc cgtatccgca aggcctgggt gggcaatcca tgtgccatcg
    24841 tgaccgttat tagcttccag cgatttatcc gcttttactt tgttgagcac ctggttattg
    24901 cgctcttcat ctttgctcgg aataaatgcc gccataccgc ccatcgcaaa agcaccgcgt
    24961 ttatggcagg ttttaatcaa caggcgtgaa taagcattca ggaatggttt atccatcgtc
    25021 actgcctgtc tgtctggcag gacgcgatcg ggatagtttt tcaacgtttt gatataactg
    25081 aagatgtaat cccaacgacc gcagttcaga ccaacaatat ggtcacgcag cgcgtgaagg
    25141 atttcgtcca tctggaacac ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg
    25201 ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca ccaggccgct
    25261 tcctgccagg actgggtttt cggcagatag aaatagggac cgctgccctt tgccaacagt
    25321 gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg gattgcctca
    25381 ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca aatcaacacc
    25441 gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct gatggtgccg
    25501 ttaactgcat cacgcaggtt aatttgcccg tcgatcactt tgttccagtc tggtgccagt
    25561 gaatcttcga aatcggccat aaagactttc acattggcgt tgagcgcgtt gatcaccatc
    25621 ttgcgctcaa ccgggccggt aatctctacc cgacgatctt gtaagtccgc gggaatacca
    25681 cgaattttcc agtcagcatc acgaatggaa gctgtttccg aaataaaatc aggcaacgtt
    25741 ccgttatcga tatcctgctg ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc
    25801 gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag aatttgcttc
    25861 tcctgctcgc catacggcct tatgaaagcc agttcatcgg ttgttgttgc ctgttcagtc
    25921 atcgtgcagc tcctcgtcat ggatccgaaa acttccccac tgtgaacgaa ggatcgttgt
    25981 gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaatca aaaacaattt
    26041 ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa atttattcac
    26101 ctactgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg taaggtgctg
    26161 aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca tgtgccgtag
    26221 atcgtatggc gtgatctggt agacgtaata gttgagccag ttggtaaaca gcaaattacc
    26281 gtgactacgc cagctcgctc gcggtgtatt ttgcggatca ttatgcggaa aatagttata
    26341 cggtacatcc gggtccagtc cggcttccac atcgcggaaa aattcctgcg ccagcgtttg
    26401 cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc tggcaaacag
    26461 atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtataat cacgaatcaa
    26521 cgctgccgga aagtcagcat agcgcgaatg tggtgccagg aatgaatcat caaagccacg
    26581 cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga gtttgtcggt
    26641 gcgggtttgc ttaggaatgc cgtagaggat attgagcgcg gcctggaccg cccaacagac
    26701 aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga tctgcggcca
    26761 gtaagcgaca tcattaaact ccaccaggcc cagcggcgcg ccggtaacta tcagaccgtc
    26821 aaagttttgt tcctgaatat cttcaaagtt acagtagaag ttattcagat gctctgcggg
    26881 cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct gcaaaggtga
    26941 gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca tcaggtttag
    27001 gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg tcatcacaaa
    27061 aacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac gaatcggcat
    27121 aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc tgaagataac
    27181 cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca aggaaaaggc
    27241 tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat tcgccgttcg
    27301 cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga tgccacccac
    27361 gattttttat cagcagagta tcgggtcgag ctggaagagt tggttcgttc ctttctgccg
    27421 gaagcaccgt tgtgggtcgc ggttaatgag cgggatcggc cggttgggtt tatgttgtta
    27481 agtgggcagc atatggatgc gctgtttatc gatcctgatg tgcgcggctg cggcgtgggt
    27541 cggatgctgg tggaacatgc gctctcgatg gcaccggaac tgacaaccaa tgttaatgag
    27601 caaaataagc aggctgttgg gttctataag aagatgggtt ttaaggttac gggacgctct
    27661 gaggtggacg atttggggaa accgtatccg ttgctgaatc tggcgtatgt gggggaataa
    27721 gctgcccctg ccgtgcctgc ccaggctgga gaagacgcg
//
LOCUS       DAOTRK010000058        27427 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.058, whole genome shotgun sequence.
ACCESSION   DAOTRK010000058 DAOTRK010000000
VERSION     DAOTRK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.058"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(4..1206)
                     /gene="ubiI"
                     /locus_tag="QIE41_002328"
                     /gene_synonym="visC"
     CDS             complement(4..1206)
                     /gene="ubiI"
                     /locus_tag="QIE41_002328"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDS3531208.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYN
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPYLCSIVWSLSPEEALRMQQASEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1229..2407)
                     /gene="ubiH"
                     /locus_tag="QIE41_002329"
                     /gene_synonym="visB"
     CDS             complement(1229..2407)
                     /gene="ubiH"
                     /locus_tag="QIE41_002329"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDS3531209.1"
                     /translation="MSVIIVGGGMAGTTLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATVCGVDWQQDPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2404..3729)
                     /gene="pepP"
                     /locus_tag="QIE41_002330"
     CDS             complement(2404..3729)
                     /gene="pepP"
                     /locus_tag="QIE41_002330"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDS3531210.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3755..4333)
                     /gene="ygfB"
                     /locus_tag="QIE41_002331"
     CDS             complement(3755..4333)
                     /gene="ygfB"
                     /locus_tag="QIE41_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDS3531211.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4501..4830
                     /gene="zapA"
                     /locus_tag="QIE41_002332"
     CDS             4501..4830
                     /gene="zapA"
                     /locus_tag="QIE41_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDS3531212.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4872..5055
                     /gene="ssrS"
                     /locus_tag="QIE41_002333"
     ncRNA           4872..5055
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="QIE41_002333"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5076..5678
                     /locus_tag="QIE41_002334"
     CDS             5076..5678
                     /locus_tag="QIE41_002334"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDS3531213.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVVTPSKVWEW"
     gene            complement(5780..5839)
                     /gene="ibsC"
                     /locus_tag="QIE41_002335"
     CDS             complement(5780..5839)
                     /gene="ibsC"
                     /locus_tag="QIE41_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDS3531214.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(6068..7300)
                     /gene="serA"
                     /locus_tag="QIE41_002336"
     CDS             complement(6068..7300)
                     /gene="serA"
                     /locus_tag="QIE41_002336"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDS3531215.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(7556..8215)
                     /gene="rpiA"
                     /locus_tag="QIE41_002337"
     CDS             complement(7556..8215)
                     /gene="rpiA"
                     /locus_tag="QIE41_002337"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDS3531216.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8271..8501)
                     /locus_tag="QIE41_002338"
     CDS             complement(8271..8501)
                     /locus_tag="QIE41_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531217.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            8642..9535
                     /gene="argP"
                     /locus_tag="QIE41_002339"
     CDS             8642..9535
                     /gene="argP"
                     /locus_tag="QIE41_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDS3531218.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            9738..10793
                     /locus_tag="QIE41_002340"
     CDS             9738..10793
                     /locus_tag="QIE41_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDS3531219.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAHRGYD
                     SDNPRVAGDVGKAGVAIDTGEDMKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISS
                     YHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDGFVPRLSFFFGIGMDLFMNAAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMCRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(10790..11686)
                     /locus_tag="QIE41_002341"
     CDS             complement(10790..11686)
                     /locus_tag="QIE41_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3531220.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASRFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSRDIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSANGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISDVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(11844..12584)
                     /locus_tag="QIE41_002342"
     CDS             complement(11844..12584)
                     /locus_tag="QIE41_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDS3531221.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVIFQLEPVDQPPAKTPAAH"
     gene            complement(12677..13312)
                     /gene="argO"
                     /locus_tag="QIE41_002343"
     CDS             complement(12677..13312)
                     /gene="argO"
                     /locus_tag="QIE41_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDS3531222.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(13451..14311)
                     /gene="mscS"
                     /locus_tag="QIE41_002344"
     CDS             complement(13451..14311)
                     /gene="mscS"
                     /locus_tag="QIE41_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDS3531223.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(14502..15581)
                     /gene="fbaA"
                     /locus_tag="QIE41_002345"
     CDS             complement(14502..15581)
                     /gene="fbaA"
                     /locus_tag="QIE41_002345"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDS3531224.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(15795..16958)
                     /gene="pgk"
                     /locus_tag="QIE41_002346"
     CDS             complement(15795..16958)
                     /gene="pgk"
                     /locus_tag="QIE41_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDS3531225.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(17008..18027)
                     /gene="epd"
                     /locus_tag="QIE41_002347"
     CDS             complement(17008..18027)
                     /gene="epd"
                     /locus_tag="QIE41_002347"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDS3531226.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            18399..18827
                     /locus_tag="QIE41_002348"
     CDS             18399..18827
                     /locus_tag="QIE41_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDS3531227.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQSCALSGYDELHISPR"
     gene            18854..19432
                     /locus_tag="QIE41_002349"
     CDS             18854..19432
                     /locus_tag="QIE41_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDS3531228.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            19433..20140
                     /locus_tag="QIE41_002350"
     CDS             19433..20140
                     /locus_tag="QIE41_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDS3531229.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIAPKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     AMILLMLAEFGAWIWLR"
     gene            20128..20805
                     /locus_tag="QIE41_002351"
     CDS             20128..20805
                     /locus_tag="QIE41_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDS3531230.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPFNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACATAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            20799..21476
                     /locus_tag="QIE41_002352"
     CDS             20799..21476
                     /locus_tag="QIE41_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDS3531231.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(21448..22161)
                     /locus_tag="QIE41_002353"
     CDS             complement(21448..22161)
                     /locus_tag="QIE41_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDS3531232.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(22158..22667)
                     /gene="fumE"
                     /locus_tag="QIE41_002354"
     CDS             complement(22158..22667)
                     /gene="fumE"
                     /locus_tag="QIE41_002354"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDS3531233.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(22689..23004)
                     /locus_tag="QIE41_002355"
                     /pseudo
     CDS             complement(22689..23004)
                     /locus_tag="QIE41_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            complement(23001..24278)
                     /locus_tag="QIE41_002356"
     CDS             complement(23001..24278)
                     /locus_tag="QIE41_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDS3531234.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLTQV
                     QKLLPVELAASKGIELVYMNTKGVSDPVQTLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(24293..25681)
                     /gene="cmtA"
                     /locus_tag="QIE41_002357"
     CDS             complement(24293..25681)
                     /gene="cmtA"
                     /locus_tag="QIE41_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDS3531235.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTTEHKH"
     gene            complement(25709..26152)
                     /gene="cmtB"
                     /locus_tag="QIE41_002358"
     CDS             complement(25709..26152)
                     /gene="cmtB"
                     /locus_tag="QIE41_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDS3531236.1"
                     /translation="MRLIDYFPKSSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKGSTINNGPYYILAPGVAMPHARSECGALKTGMSLTLLKQSVYFPGNDEPIKLLIG
                     LSAADANSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            26529..>27427
                     /locus_tag="QIE41_002359"
     CDS             26529..>27427
                     /locus_tag="QIE41_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDS3531237.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFIS"
BASE COUNT         6771 a         7334 c         6671 g         6651 t
ORIGIN      
        1 tatttaacgc agccattcag gcaaatcgtt taatcccatt gcctgacgaa taagttgtgg
       61 cttaacgcca ggaagcgtat cagccagttt cagaccaata tcacgcagca attttttcgc
      121 cggattagcc ccggaaaaca gatcgcggaa tccctgcata ccagccagca tcagcgccgc
      181 gctgtgcttg cggctacgct catagcgacg caggtaaatg tactgcccga tgtctttacc
      241 ctgacgatgc aaccgtttca gttcagcaat cagctctgca gcatccataa agccgagatt
      301 taccccctgc cccgccaacg ggtgaatggt atgcgcggcg tcgcccacca gcgccagacg
      361 gtgcgcggca aactggcgcg cataacgccc cgttagcggg aacaccaggc gctcactttc
      421 aaccttgcat aagcccaggc gattatcaaa agcgatattt aacgcgcgat taaattcgtc
      481 ttcactagcc tgctgcatcc gcagcgcttc ctctggcgac agtgaccaga caatcgagca
      541 aagatacgga tcgctaagcg gtaaaaaggc cagaatgcct tcgccatgga aaacctgccg
      601 cgccaccgca tcatgcggtt cttccgtgcg aatggtcgcc accagcgcgt gatgctgata
      661 atcccagaaa gtcagcggaa tatcggcttt gttgcgcaac caggaattag cgccgtctgc
      721 gccaatcacc agacgcgccg ttaacatgct gccatctttc aacgtcagga aggtttcatt
      781 ttctccccag gcgacctgct gtaattctgc gggggccaac agagtgatat ctgacgactg
      841 ctgcgctttg ttccacagcg cgtagtgaat cactgagttt tcaacgatat gcccaagatg
      901 gttatagccc atgctttgat cgtcaaacga gatgtgacca aaactgtctt tgtcccacac
      961 ttccataccg tgatagcaac tggccctacg agagagaatg tcctgccaga cgccaagacg
     1021 agtcagtaat ttttcgctgg cggcattgat agccgaaacg cgcagttgtg gtggtgcatc
     1081 cgccgccaga ggttccggta cgcgctgctc cagtacggca acgcgtaagc cgctaccctg
     1141 taagccgcag gcaaccgcca gcccgaccat gccgccgcca acaatggcta catcaacact
     1201 ttgcattgtt tattccttaa aaccgccttc aacgcgccac ccaaccaaga gtgcgctgcg
     1261 ccagcacatc gcgtgctggg gtaaataatt ccatcgtcat cagcccgatg ttgcgcccga
     1321 caaccagcgg cgtccaacgg ttggcaaaaa gatgtacaag gctgtccgtg acgccgatgg
     1381 ttgcttcgcg atcgctctgt cgacgctgct gataacggca caatacgccg taatcaccca
     1441 tgtcttctcc gcgttcctgc gcctgagtca gggtttccgc aaggctcatg acatcacgca
     1501 taccgaggtt aaatccttgc ccggcgatgg gatgcagagt ttgcgccgca ttgcccacca
     1561 gcacggtacg atgggtaata ggtttggcgg cgcgggttag cgctagcgga taagcactgc
     1621 gtttaccggc atgagtaatt ttcccaagtc gccagccaaa ggccgactgg agttcacggc
     1681 aaaacttctc gtcgctccac gacagcacct cttcgcgccg ttccagtgga tgacaccaga
     1741 ccagcgaaca gcgtccgtca gacatcggca acatcgccag cgggccatgt tgcgtaaagc
     1801 gttcaaaagc gcgcccttca tgcgcaacgg aagtcgcaac gttcgcaatc acagccagtt
     1861 gttcgtaagg atcctgctgc cagtcaacgc cgcacacggt ggctaacgct gaatgggtgc
     1921 catcagcggc taccagcacg cggcccgtca gcgtctcacc actctccagc gtcacttcaa
     1981 cgtggctctg agtacgggca acgttagcca cgcgatcagg gcaatgcagc gttacaccag
     2041 gtgctttgcg cagcaatgca aacagccgtt gcccgacatt gtgcaattcg acaacctgcc
     2101 ccagcgccgc cagttggtaa tcttctgcgg cgagggtgac aaatccagcg tgaccacgat
     2161 cgctgacatg cactgtggtg atggcagttg cgcaatcagc cagagattgc cagacgccga
     2221 tgcgcgccag ttgctgacag gtacccgccg ccagcgcaat agctcgtcca tcaaagcccg
     2281 gatgcgcatg tgactctggc gcggtcgctt caatcaaatg tactggcagc gccccgtgac
     2341 ttaaccggga aatagccagc gccagcgtcg tgcccgccat gccgccaccg acgatgatta
     2401 cgctcattgc tttctcgcag ctgccatcaa cgcttcgatt tcttccggct ttttcaccac
     2461 gctggcggtg aggttttcat taccggtttc ggtaatcaca atgtcgtctt caatacgaat
     2521 gccgataccg cgatactgtt ctggcacttc tgcatccggc gcaatataca gccctggttc
     2581 tacggtcaga accatacccg gttccagaat acgcgagcga tcctgaccat aaacaccaac
     2641 gtcatgaaca tccagtccta accagtggct aaggccatgc ataaagaaag gacgatgggc
     2701 gttctgagcg atcagttcat caacatcacc tttcaggata ccgagtttta ccagaccgct
     2761 aaccatgatg cgcaccactt caccagtgac ttcctgaatg gaagttcccg gacgatacag
     2821 acgcaggctg gtttcaagag actccagcac aatgtcatag atttcacgct gagcctgagt
     2881 gaatttgccg ttgaccggga aggtgcgggt aatgtcgcca gcgtaacctt tgtattcaca
     2941 acccgcgtca atcaacacca ggtcaccgtc acgcagttca cactcgtttt cggtgtagtg
     3001 caaaatgcag ccgttttcac cgctgccaac aatggtgtta taggaagggt aacgcgcacc
     3061 gtggcggtta aattcgtggt gaatttcgcc ttccagatgg tactcgaaca tccccggacg
     3121 gcatttttcc atcgcccgtg tatgcgccag tgcggtgatt tcacccgcgc ggcgaagtac
     3181 ggcaatctct tctggtgatt tgaacaggcg catttcatga acaacaggac gccagtcgat
     3241 catcgttgcc ggtgcggtga gattttgccg cgaaccttta cgcagtttct caagcgcgct
     3301 gttcacgatt tcatcagcat atgcatattc gccctgggcg tggtaaacca catccagacc
     3361 attaagtagt tgataaagtt gctgattgat ttcgctgaat gccagtgcgc ggtcaacgcc
     3421 cagtttctct ggcgcggcat cctggcctaa gcgacgacca aaccagatct ctgccgtcag
     3481 gtcgcgaacg cggttaaaca aaacgctgtg gttatgagtg tcatcgcttt taatcagcac
     3541 cagcaccgct tccggttcgt taaagccggt gaagtaccag aagtcactgt tctgacgata
     3601 ggggtattcg ctgtcggcgc tacgtgttac ttctggtgca gcaaaaatca gcgcggcgct
     3661 gccgggttgc atttgctcta ccagggcctg acggcgacgc tgaaactctt gccgggatat
     3721 atcactcata acactctcct tacatttttt gtttttagtg tagagtcggt ttttgtactt
     3781 ctggcgcggt cggttgcgga tgagtaaagg tgtcgtggca taacagcgcg gcaacacgga
     3841 cgtactcgat gatctcttca agcgacattt caagctcttc ctgatcttcg tcttcgtcgt
     3901 aacccaactg cgcgatgtta cgcagatcgt cgatggcttc accggtttcg ccggtcactt
     3961 tgtccagctt cggttgcgta acgccaagac caagcaggaa gtgattgacc caaccagcca
     4021 gcgcatcagc ccgatcgaaa acgctgacat catcgccatc aggcagataa agctgaaaaa
     4081 ggaagccgtc atcctgcagg gcatcgctgg tggcagagtg cattttgcgc agtgcctgtg
     4141 ccagctcatg accgaaagcc atgccttcgt tcgtcaggtc gtgaagtagc ggcagccatg
     4201 agctgtcatc gttaccgcca catatcatcc cactgattaa accatgcatc tcagcagggg
     4261 tcagacccgt cccttgttgg ttcagatact ggttcatttc gttgtaacca ggcatttcgt
     4321 tctgtataga cataagcatt cgtcgtcaaa gggaggaata ttcatgatat gctaccactt
     4381 tgggccctgg tggaccagaa aagggcttgt ctcttctcat cagggtagct atagtgtcgc
     4441 cccttcgcag accatgggtc taaagacgaa ggcagcgcag tcaatcagca ggaaggtggc
     4501 atgtctgcac aacccgtcga tatccaaatt tttggccgtt cactgcgtgt gaactgcccg
     4561 cctgaccaaa gggatgcgtt gaatcaggca gcggacgatc tgaaccaacg gttgcaagat
     4621 ctgaaagaac gcactagagt cacaaatact gaacagttgg tcttcattgc cgcattgaat
     4681 atcagctatg agttagcgca agaaaaagcg aagactcgtg actacgcagc aagtatggaa
     4741 cagcgtattc ggatgctgca gcagaccata gaacaagcgt tacttgaaca aggtcgcatc
     4801 accgaaaaaa ctaaccaaaa ctttgaatga cacttttcgg tttactgtgg tagagtaacc
     4861 gtgaagacaa aatttctctg agatgttcgc aagcgggcca gtcccctgag ccgatatttc
     4921 ataccacaag aatgtggcgc tccgcggttg gtgagcatgc tcggtccgtc cgagaagcct
     4981 taaaactgcg acgacacatt caccttgaac caagggttca agggttacag cctgcggcgg
     5041 catctcggag attcccttct tacctggcac cagccatgac gcaactacca gaactcccac
     5101 tgacattatc ccgacaagaa atccgcaaaa tgattcggca acgtcgtcgt acgttaacgc
     5161 cggaacaaca gcaggaaatg ggtcaacaag ccgctacccg gatgatgacg tatcccccag
     5221 tggtgatggc gcatacggtc gctgtattcc tctcttttga tggcgaactc gacacccagc
     5281 cactcataga acaactctgg cgcgccggta agcgcgtata tcttccagtt ttgcatccct
     5341 ttagtgccgg taacttgctg ttcctgaatt accatccgca aagcgaactg gtgatgaaca
     5401 ggttgaagat ccacgagcca aaactggacg tgcgcgatgt gcttcccctt tcccggttag
     5461 atgtattgat cacgccgctg gtggcttttg atgaatacgg tcaacgcttg ggaatgggcg
     5521 gtggttttta tgatcggacc ttacaaaact ggcagcacta taaaatgcaa ccggtgggtt
     5581 atgcgcatga ttgccagtta gtagaaaaac tcccagtgga gaaatgggat attccactgc
     5641 ctgcggtggt tacaccgtcg aaagtctggg aatggtaagg gcgatacatc ccgcatcgcc
     5701 ctgattgaca tcgttgattc tttgacctaa tttagtgagt aagggtaagg gaggattgtt
     5761 cctcccctgt gactgactgt taataagctg ggaaacttat gagtaacagt acaatcagta
     5821 tgatgacaag tcgcatcata acccttctcc ttcaagccct cgcttcggtg agggctttac
     5881 cgttacagcc ccatgctgcc ctgccatcgt aaatccccat taaataaaca caacgcattg
     5941 atctgacttt gatttatttt ctggagcaga ctcgcaaagt agaatgcgca atgcggcaac
     6001 ggtgtggaga agggataaaa aaacgggcaa gtcagtgacc tgcccgttaa ttttcagaga
     6061 aggggaatta gtacagcaga cgggcgcgaa tggtacccgg aatagctttc attgcctgca
     6121 gcgctttttc ggcaacgtct tcgtcggctt caatatcaat aactacataa cccatctggg
     6181 cggaagtttg tagatattgc gcggcgatgt tgacgccctg ctcggcaaaa attttgttga
     6241 gcgcagttag cacgcccgga cggttttcgt ggatgtgcat cagacgacgc ccaccgtgca
     6301 gtggcagcga gacttccggg aagttcaccg cagagagcgt tgagccattg tcagaatact
     6361 tgatcaattt acccgcaact tccaagccga tattctcctg cgcttcctga gtcgaaccgc
     6421 caatgtgtgg cgtcagaagg acattgtcga attcacacag cggagaggta aatggatcgc
     6481 tattggtcgc cggttccgtc gggaatacgt cgattgccgc ccccgccaga tgtttgctcg
     6541 ccagcgcgtc acacagcgct ggaatatcca ccacagtacc gcgcgaagca ttaatcagca
     6601 gcgagccggg cttcattagc gaaatctctt tcgcgcccat catatttttg gtggacggat
     6661 tctctggtac atgcagactc accacatcgc tcatattcag caggtcagaa agatgctgta
     6721 cctgagtggc gttgcccagc ggcagtttgt tttcaatatc ataaaagtaa acatacattc
     6781 ccagcgattc agccagaatg cccaattgcg taccaatatg accgtagccg atgataccca
     6841 gctttttgcc gcgcgcttca aaagaacccg ccgccagttt gttccacacg ccacgatgcg
     6901 ctttagcatt ggcttctggc acgccgcgca atagcagcag cagttcgcca atcaccagct
     6961 ccgcaacaga gcgcgtattt gagaacggtg cgttaaatac cgggatcccg cgctttgccg
     7021 ccgcatccag atcaacctga tttgttccga tacagaaaca gccaatagcg accagttttt
     7081 ctgcggcgtt gatcacgtct tcagtcagat gggtacggga tcgcaggccg atgaagtggg
     7141 catcgcggat ggattctttt aattgttcat catccagcgc gcctttgtga aattcgatgt
     7201 tggtgtaacc agctgcacga aggctttcca gcgccttttg gtgcacgcct tctaccagca
     7261 gaaacttaat cttgtctttc tccagcgata cctttgccat ttacccaatc ctgtcttttg
     7321 aaatgttgtg tgcggatttg catccgcctt tcaacatatc aaaaaataat attgcggcaa
     7381 tatgaacgtt tgcgtcgcga tgttgaggaa atatcacgca aaagtatttc ttggatgaat
     7441 atgctggttt agaagattta tttagagcaa tcgacaattg ccaggtaaaa acgtgacaca
     7501 tgtcaccaaa tttaatgaag agaatttttt taacggggga ggttcccccg tcagatcatt
     7561 tcacaatagt tttgacaccg tcaggtgtgc caatcagcgc aacgtccgcg ccacggttag
     7621 caaacaaacc aacagtcacc acaccaggaa tcgcatttat ggcgttttcc atcgctatcg
     7681 ggtcaaggat ttccatgccg tggacgtcga ggatcacgtt gccgttatcg gtcactacgc
     7741 cctgacggta ttccggacga ccgcccagtt tcaccagctg gcgcgccact gcactacgtg
     7801 ccatcgggat aacttctact ggcagcggga atttgcccag aatatcaacc tgcttggaag
     7861 cgtctgcaat acagataaat ttttccgcaa ccgaagcaat gattttttca cgggtcagcg
     7921 ccgcgccgcc gcctttgatc atttgcatgt ggccgttgat ttcatctgcg ccatcaacgt
     7981 agatgccaag gctgtcgact tcgttgagat caaaaacgtg aatgccgagg cttttcagtt
     8041 tttcagtgga agcatctgaa ctggaaacgg ccccttcaat ctggcctttc attgtaccga
     8101 gtgcgtcaat aaagtgtgcg gcggtggaac ctgtacctac accaacaatg gtgccgggct
     8161 gaacatactg aagtgccgcc catcctactg cttttttcaa ttcatcctgc gtcatgatcg
     8221 tttcgcctgt ggtatgaaat ttcacacgca ttatatacaa aaaaagtgat tcagaccccg
     8281 ttggcaagcc gcgtggttaa ctcatccata aaatatcgcg caatggcggg catccccttt
     8341 cgccccgcaa ataaagcata caacggtctg gggatgccgc tccacggtgc aaacaggcgc
     8401 accagttccc cgttcgcaag cccctgttta caggcaaact gaggcaataa cgccacgcca
     8461 ttcaacacaa ccagggcgcg aactcgctga gcgaaatgca ttagcgcaaa tccctgatct
     8521 tttaagctgc gcgttaacca ctgatacaaa tgttacccgc caacggcttc ggtatatgca
     8581 acccgacaca aaattgtgtc atagtgcagg aaaaagcatt taccaggagc agacaacagc
     8641 aatgaaacgc ccggactaca gaacattaca ggcactggat gcggtgatac gtgaacgagg
     8701 atttgagcgc gcggcacaaa agctgtgcat tacacaatca gccgtctcac agcgcattaa
     8761 gcaactggaa aatatgttcg ggcagccgct gttagtgcgt accgtaccgc cgcgcccgac
     8821 ggaacaaggg caaaaactgt tggcgctact acgccaggtg gagctgctgg aagaagagtg
     8881 gctgggcgat gaacaaaccg gttcgactcc gctgctgctt tcactggcgg tcaacgccga
     8941 cagtctggcg acgtggttgc ttccggcact ggctcctgtg ttggctgatt cgcccatccg
     9001 cctcaacttg caggtagaag atgaaacccg cactcaggaa cgtctgcgtc gcggcgaagt
     9061 ggtcggcgca gtgagtattc aacatcaggc gctgccgagt tgtcttgtcg ataaactcgg
     9121 tgcgctcgac tatctgttcg tcagctcaaa accgtttgcc gaaaaatatt tccctaacgg
     9181 cgtaacgcgt tcggcattac tgaaagcgcc agtggtcgcg tttgaccatc ttgacgatat
     9241 gcaccaggcc tttttgcagc aaaatttcga tctgcctcct ggcagcgtac cctgccatat
     9301 cgttaattct tcggaagcgt tcgtgcaact tgctcgtcag ggcaccacct gctgtatgat
     9361 cccgcacctg caaatcgaga aagagctggc cagcggcgaa ctgattgact taacgccagg
     9421 gctattccaa cgccggatgc tctactggca ccgctttgct cctgaaagcc gcatgatgcg
     9481 taaagtcact gatgcgttgc tcgattatgg tcacaaagtt cttcgtcagg attaatccat
     9541 caaataatgc ctgatagcac atatcaggcg ttatcctcac ttctttttgt attccttgaa
     9601 tcacattaca aaatagacaa atctcaggcg gcaaaaaacg acgtctgaat gcattttttt
     9661 gctggagaca aacccacgta aaaagctcac cgtaagcgca aataccctca ttttgattgc
     9721 gttttacgga gcaaataatg tctaacgtgc aggagtggca acagcttgcc aacaaggaat
     9781 tgagccgtcg ggagaaaact gtcgactcgc tggttcagca aaccgcggaa gggatcgcca
     9841 tcaagccgct gtataccgaa gccgatctcg ataatctgga agtgacgggt acccttcccg
     9901 gtttaccacc ctacgttcgt ggcccgcgtg ccactatgta taccgcccaa ccgtggacca
     9961 tccgtcagta tgctggtttc tcgacagcaa aagagtccaa cgctttttat cgccgtaacc
    10021 tggccgccca ccgaggctac gactccgata acccacgcgt ggcgggcgac gtcggcaaag
    10081 cgggcgtcgc tatcgacacc ggggaagata tgaaagtcct gttcgaccag atcccgctgg
    10141 ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg gcgttttata
    10201 tcgtcgccgc agaagagcaa ggtgttacat ccgataaact gaccggcacc attcagaacg
    10261 atatcctcaa agagtacctc tgccgcaata cctatattta cccgccaaaa ccgtcaatgc
    10321 gtattatcgc agacatcatc gcctggtgtt ccggcaacat gccgcgattt aataccatca
    10381 gtatcagcag ttaccacatg ggtgaagcgg gtgccaactg cgtgcagcag gtagcattta
    10441 cgctcgccga taggattgag tacatcaaag cagcaatctc cgccgggctg aaaattgatg
    10501 gcttcgtccc tcgcctgtcg tttttcttcg gcattggcat ggatctgttt atgaacgccg
    10561 ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt ggcgcacagg
    10621 atccgtataa caacgttatc cgcaccacca ttgatcattg tgcacatcct atgtgtcggg
    10681 attatctgca tcgctatctg gaaaatgcgt ccggcggaca tattcaccac gatcttagcc
    10741 acgtcttcga cttacaccgt aatttaattg caaccggatc gatgctgggt taatggtttg
    10801 tcccgtcacg atcttcagca atatgctgaa gcatcgtggc ggtataacga tgatttctga
    10861 ttgaataaag atattcctgc atatacatcg gcctgtgcgg cgcatcaaaa tatttcagcg
    10921 ttgccggatt caccagtcgc caggcaaaat gcgggatcac cgtcagaaaa tgtccccgct
    10981 ccacgtcgct gattttggcc ataaaactat agggacgata aataatggtt ggattgatcc
    11041 cacaaggacg catattcgca tcaatcattg cttcgaagtt agcccggttt tgaaagcgca
    11101 tttgtagcca gggcagctcc tgaagcagct cttgctcttg tcgctcttca taacggcgag
    11161 aaacgagaaa gcccaatcgc agcggcggta gttcactgat agtcaggttt tccagttcct
    11221 gaactcgtgc agatacatgc tgtggggaga ttataaaatc taactgacgg tcaaaaagat
    11281 tatcgataac gccatttgcg ctgaactcaa ctggctgggc ggtcacgcct tcgtacttat
    11341 ctccgagact aatcaactga tcaaaaataa tggtcggata agtattatct atgcctatca
    11401 caatgtctcg tgaacgacgt cctgagttat ggatttcatt atcaatcgca gaaagtcgtt
    11461 gataaaccgg gaacagtttt tgatacagtt cctgtccggc tttattgagg ctgatattat
    11521 tatctttgcg ggtaaatagc gtatagccaa tttgttcttc cagcgcggca atgcttttgc
    11581 caaatcgcga agccgtcata tggatttttt ctgccgcccg ggcaatatta ttggtttgcg
    11641 ctaataaaat gaaattgcgc atctttttcg aaataaaaat gtccatcccc cctctcccgc
    11701 atccttgtcc gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata
    11761 ggtgattacg tcatggtttt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
    11821 acgaatacgt cgggctacaa gtattaatgt gcagcaggtg ttttagcggg tggttgatcc
    11881 acaggttcta actggaagat tacatcgacc tgatcatcaa actgaatagc ggcctgctcg
    11941 taagtttcct gggcggacac cggcgcggca tcggctttca tcatccgcac cattgggctg
    12001 ggctgatagt tggaaacatg gtagcgtacg ctatataccg gccccagttt acgatgaaag
    12061 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
    12121 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
    12181 gcctttagcg cgccgtccag caaggaattc agtttgtcta actgacggag cgtgacttcc
    12241 accgttctca ctgcgcggta gcctttcagg atacttttac catcctgata atcataatct
    12301 ggctgagtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagctcaagg
    12361 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggcagtagc ggcatccttc
    12421 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacgcttgcc
    12481 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
    12541 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
    12601 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
    12661 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
    12721 gctgcaaggc aataaaccac ataacacatc ccactaccag attgataatg cgctgtgatt
    12781 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
    12841 ggaaagaggc gctaattgtt ccgagcgcaa accaacgttt tggttccaca tcaagttgcc
    12901 cgccaaggct gcccagcaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
    12961 ctgccaacat ggtggcgata attttccatc tgccttgttt caggacttcg gcactggcta
    13021 actcaatgtt actgctcatt gctgttttaa aagcgccaaa accataccac agcaagaagg
    13081 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
    13141 caccaaaaat ccccgcacaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
    13201 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
    13261 gtaggatcat agccgcccca agtgcaagac cttgaaagta ataagaaaac acgttggata
    13321 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
    13381 taatattaat tggcaatcag aaaaactaat aagagagagg ccagagagta ttatctggcc
    13441 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
    13501 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
    13561 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
    13621 atgcgccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
    13681 actggataat atcggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
    13741 caataataaa ttcgttacgg cgagctggct cgcgggagaa gttaataata ttcccggcaa
    13801 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
    13861 aaatctgcac actcagcaca gtaccggcta cgccgccgag gtcaacatat tctccggcac
    13921 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagacagt gacccctgca
    13981 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
    14041 cgcccacgcg ccccagtgca gcgattagcg taaaggcgat aattccgtaa cggactaatg
    14101 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
    14161 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
    14221 tgtttactgc ataacttagc agcagcgcct ggttagccac cagccagctt cccgcgccgt
    14281 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
    14341 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
    14401 aattttgcca aatagatcac agatttctca tctgtatcac cttgcttttg gcgaaaaaaa
    14461 gacccgcaga gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg
    14521 ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc
    14581 catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttgcccagc
    14641 tggccttgca ggtaagcttc gttttctttg tagtagttca gaacaccttc ccaggttgcc
    14701 cattgggtat cggtatcgat gttcattttc actacgccgt agcttacgga gtctttgatt
    14761 tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg
    14821 ttgtgtttct tagaaacata ttcctgagag tcgcgcagga tggtcggagt cagaacaacg
    14881 ttacccggct tgtaaactcc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg
    14941 ctgattttgc tcagctcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca
    15001 gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg
    15061 atttccagag tcatgccgat tttggacatg cgctccaggt atttagagca gatttcaatg
    15121 ttctcttgca gagattcttc agacaggtca atcatgtgag aagagaacag cggcttaccg
    15181 gtagcagcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc
    15241 ttcgcgcagt ggtcggtgtg caggataacc ggaacaccat aatgttcagc catctggtga
    15301 acgtgatgcg caccagagat cgcacccagg atagcagcgc cctgcggaac gtcagatttc
    15361 acgcctttac cagcgataaa agaagcacca ccgttggaga actgaacgat aaccggcgct
    15421 ttaactttag ctgcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact
    15481 gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca
    15541 gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt
    15601 cgggccttat actcgtcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
    15661 gtcacttact tatgtaagct catgggattc actctcttgt cgccttcctg caactcgaat
    15721 tatttagagt atgaaaaagt gcgtgcttta aaagcgcgct gcaacaaggg caggtttccc
    15781 tgccctgtga ttttttactt cttagcgcgc tcttcgagca tcgctactgc aggcagtact
    15841 ttaccttcca cgaattcgag gaatgcgccg ccgccagtgg agatgtagga gattttgtca
    15901 gcaatgccga acaggtcgat tgctgccaga gtgtcgccgc cgccagcgat ggagaacgct
    15961 tcgctgtctg cgatagcgtt agccacgatt tcagtacctt tgcggaagtt cgggaattcg
    16021 aacacgccaa ccggaccgtt ccacaaaatg gttttcgcat tcttcaggat ttcagccagt
    16081 tcctgagcgg aagcatcacc gatatccagg atctgctcgt cagctttcac atcgttaaca
    16141 gatttcaggg tagccggtgc agtttcagag aactcggttg ctacgcgaac atcagacgga
    16201 accgggatgt tgcaggtggt cagcagacgt ttagcttcgt caaccaggtc agcttcgtac
    16261 agggatttac ccacatcgtg gccttgtgct gcgataaagg tgttagcaat accaccacca
    16321 acgatcagct ggtcagcgat tttagacagg gagtccagaa cggtcagttt agtagatact
    16381 ttagaaccac caacgatagc caccatcggg cgagcaggtt ctttcagtgc tttacccagc
    16441 gcgtccagtt cagctgccag cagcgggcct gcgcacgcaa cgtcagcgaa tttaccgata
    16501 ccgtgagtag aagcctgcgc gcggtgagca gtaccgaatg cgtccattac gaacacgtca
    16561 cacagtgcag cgtatttttt ggacagagtt tcgtcatctt tcttctcgcc tttgttgaag
    16621 cgaacgtttt ccagaacaac cagttcacct tcagcaacgt caacgccgtc gaggtaatct
    16681 ttaaccagac gaaccgggtt agacagtttg tctttcaggt agttaacaac cggcagcaga
    16741 gagaattctt cgttgtactc gccttcggta ggacgaccca ggtgggaagt taccatcact
    16801 tttgcgcctt gtttcagggc aagttcgatg gtcggcagag aagcacggat acgcgcgtcg
    16861 ctggttactt tcccgtcttt tactggtacg ttcagatccg cacggataaa tacacgtttc
    16921 ccagcaagat ccagatcggt catcttaatt acagacatgg tgaatcctct cgttgattct
    16981 aaaagttttg cagacgctgc ttgcgtctta cctgaaagca acagtagcca tagctaacgt
    17041 cgtgtcgagc attcggttag caaagcccca ttcgttatcg caccagacca acgttttgat
    17101 caggtgtgcg ccactgaccc gggtttgggt gccatcgaca atggcactgt gcggatcgtg
    17161 gttaaaatct acagagacca acggcaattc cgtatagtca actataccat gaaatgcacc
    17221 ttgtgctgct ttttgcagca acaggttgac ttcattggct tttacaggtt tcttcaccgt
    17281 cacgcttaaa tcgattgccg tcacatttat ggttggcaca cgtaccgcaa tcgcttcaaa
    17341 gcgatcgtta aattgcggaa aaaatcgtgt gataccggcg gccagtttag tatcgaccgg
    17401 aatgatcgac tggctggctg cccgggtgcg acgcaggtca ggatggtatg catcaataac
    17461 ctgttgatcg tgcatggcgg agtgaattgt ggtcacagtg ccggactcaa taccgtacgc
    17521 atcatctaac aatttgatga cgggaattat gcaattcgtg gtacaggaag cgttagaaac
    17581 gatgcggtgt tccgcacgaa gttgatcctg attgacgccg taaacaacgg tcgcgtcgag
    17641 atcgttactg ccaggatgtg aaaagagcac ttttttggcc ccggcggcaa tatgcgcttc
    17701 gccatgctca cgggagccat atacgccggt gcagtcgagg actacatcaa cgccaagttc
    17761 acgccagggg agcgattgca gtgaacgttc atgcaatacg cggatggcgt catcaccaac
    17821 aaaaagttga tcgcgttcct gtcgtacttc ccatgcaaaa cggccatggc tggtgtcata
    17881 tttcaacaaa tgcgccatgc ccgcagcatc cgccagttcg ttgattgcca ccacggtaat
    17941 ttccgcccgg cgtccggatt catacaaagc acgaaccaca ttacgcccga tgcgaccgaa
    18001 gccatttatc gctacgcgta cggtcataga tctcctgcaa ggttttccct gagcaaattt
    18061 gccagacaga gtaatccagc aaatcgttcg gggaaacctt acctgtcgca aactgcgact
    18121 gattggttaa ttgtcgaaca tttaatcgac tgaaacgctt cagctaggat aagcgaaacg
    18181 tggaataaaa ggaatgattg tccagtcaaa gaagacaatt atctgactta cgtcacgttt
    18241 tcagccccgc ccctgtggaa tttattccac tttgcagaat cacgaataag cgtagcaaaa
    18301 tcttttcgtt gtgccgtcac aaaccctgac ttatatcaga atatgactga tgcagcgtcg
    18361 atattatccg caatacgtcc gagtttcgga gtactccaat gatgacattg ccctacccat
    18421 attcttcctc cgcacgtttt tacgcgctgc gcctgctccc cgggcaggaa gttctctccc
    18481 aattacgcgc cttcgcgcag caacaacaac tccacgccgc gtggattgca ggttgtaccg
    18541 gcagtctgac tgacgtcgcc ttacgctatg ccggacaaga aaacactgcc ctgctaagcg
    18601 gtaagttcga agtcatcgca ttgaacggca cgctggaaca gagtggtgaa catcttcatc
    18661 tctgcgtttc cgatccacac ggcacgatgc ttggcggtca tatgatgccc ggctgtaccg
    18721 tgcggaccac gcttgaactg gtgatcggct gccttgagga actggcgttt agccgccagt
    18781 cttgcgcgct ttctggttac gacgagttgc atatttcccc ccgttaaata actactttca
    18841 gtgaggtttt cttatggcgc gtcgccactt ttccagtcag gcattggtgt tgatcgttat
    18901 ctctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat tacctatttt
    18961 tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga tcgggatgct
    19021 aaccggttta ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt
    19081 tgcgcccgtc gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt
    19141 tcgcaccctg ccaaaagtcg ttgttagcgg cgtgattatt acgcttgcgg taacggtcgt
    19201 cgctgtaccg ctgcgtactg cgctgttcgg cggcgtgaca ggaagcggag ccgatttgtt
    19261 cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat
    19321 aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgtca
    19381 gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc
    19441 gtttacatcc ttaacgctgt gggcgctggc ggcctgcacc acgcttatcc tgcccacaca
    19501 gacaatattg ccagtctata gcgcggcgac ctttttctgc cttattgcgc caaaggccac
    19561 gcggcggcgg gcaaaatatg tcgtctggct gatgttctcg ctgggggctg gactgtggct
    19621 ggtgcacggt ggctggctga cagaatggct aagcggcacg ccacgctctc cagaacggtg
    19681 ggcgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg
    19741 gatgcagtac gtccccgtgc aacgttttat ccgcgctttg tttgcttctc gcctgccgcc
    19801 gggcgtcgct tatctgtttg ccgggccgtt attggtcgtt gagcagttaa aacggcaatt
    19861 ggcgattatt catgaagcgc agcgcgcccg cggcgtgccg ctggatgagg gctggtatca
    19921 gcgtttacgt gcgatgcccg cgttaattat tccgctaaca cacaatgcgc taaacgatct
    19981 tgctgtccgt ggtgcggcgc tggatatgcg ggcatttcga atcaacaata ggcgcactac
    20041 gttatgggca ccagccgata gcaccctaca acgcgtagca cgttacgcca tgatattgct
    20101 aatgctggct gaattcggag cgtggatatg gttacgttag aacagtttcg ctattgcccg
    20161 acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc
    20221 gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cgcagctgat ggcgggctgg
    20281 tatccggact ttctaccagg agagataacc ggcacgggga cgcttctcgg tacgcccatt
    20341 gggcgtctcc cgtttaatga acaatccgcc accatccagc tcgtccagca atcgccctat
    20401 ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat
    20461 ctgtgtcttg ctgaaaaaga gattatggcg cggattgacg ccgccctggc cctgaccgag
    20521 tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac acagcgtgtg
    20581 gtcatcgcct gcgccaccgc gatgcagcca aagctcttaa ttcttgatga agcctttagc
    20641 cgcctgacgc cgcaggcccg cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa
    20701 cgtggttcgc tgattattct ttttgaacgt catcacacac ctttcctgaa ccactgccag
    20761 caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat
    20821 cttatcgctg gcctggcgcg gcaacggatt gtctttgcga catttctctg caactcaaac
    20881 aaggtgaatg gctggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag
    20941 taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa caagcgatga
    21001 aaaacctgaa aaaccgccag cgcgcggcaa agattggcgt actgtttcag gaagcggaaa
    21061 atcagctttt tcacagcacc gttgctgacg aaatcgcatt tggtttgata ctgcaaaaat
    21121 gcccggcgga tgaaatcact caacgtacca atgctgccct gcaatgctgt cagttagcag
    21181 acactgccag cgcgcatccg ttagatctgc atagtgcgca gcgacggatg gtcgccgtcg
    21241 cctgcctgga agcactctct ccgccactat tgctactgga cgagccgagt cgggattttg
    21301 acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacta
    21361 gcgtcgtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc
    21421 gactggaaga tggtgtaatc aggaacgtta atccatcaga cgatattcac ccgtagcggt
    21481 catcattaag gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc
    21541 gtcggtacgg tcataaaagg cttctgcgtc tgccagcgat aaccctcccg ccagcttgcg
    21601 ccccaccaga cgctcccgca acgcagatgc gggagctttg ataaagatcg agaaatcaca
    21661 aaattgcgca agctggcacc acttttcatc atccagcaac aaccagtttc cttcgacgat
    21721 gacgagtggt gcggtaacgt gcagcgcatc ttcaacagga tcatgctttt gtcgatcgta
    21781 ctgcggccac gtacaatccc cttccacaac ccggcacaga ttttccgcca gtttcgcaac
    21841 gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcatcca gccagctatt
    21901 gtaatggtga aaaccatcca tcggcagcgt ctgaatagcg ggcagttccg ggtcttgttg
    21961 cgcgagatat tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag
    22021 aaaaaccacc gtacgccgct gcggatttac tgtctgtaag gccgccagca tacgcagcaa
    22081 cggtttatgt acattttcaa tctcttcgtt ctggtactgt gcctgtatct tcagcccatt
    22141 caccgttaat tcaattttca cggcgttaat tcctttaagg ttgtcgttac tgccagcgtc
    22201 agcgccgttt tgaccatccc gctaaaacgc gcctctgacc agacggcgaa atcggcgaac
    22261 ttcatcgctt tcaacgcgtc atagagcgtc gcattgcggg ttatgctatt gacgttgctg
    22321 ataaaatccc aggttatgtc atcagcaaat ccgggcgttt catcctgttc gttcaggtaa
    22381 tgccagaact gggaaaagtg cgtgatatcg gcatacagcc atttatccat cttccccagc
    22441 gcataaatca aacgcagcgc aacatcaata tcgtcaagcg ggccgctttg ggcgagtaac
    22501 ggcttcactg catattcgac gatctcttca tcgttatcga caaacagcga cggcagaaac
    22561 tggcgtatcc cctggagcaa aatagaatgc gcagttttca taaatgaaaa taaattgtcc
    22621 tgtgcatcca gttgctcaag cacatcatct tctgtcagcg tcgccattgg tcttccgata
    22681 gatcaccatc aatgcaggga gtctattata ttacacgttt ggtccgcgcc gccgatcagt
    22741 aatgtctgcg tccgcacccc attcgccgtc tgttggatac cgttcaccaa ctcgccgccc
    22801 gtcacgcccg tagcgctaaa gaggatatcg ttccccctcg ccgataacca ctcgcccacg
    22861 aaaagcaacg tcgtttaatg cctggcgcat cgcggtgacc gcaaggccat caattttatt
    22921 tttgtcgcca cagccggttt gcggccaggc agccaacgct gcctgttccg taacgcgaaa
    22981 taatggccac gccagggaca tcattgcgcg gcctccccaa tatcaacgcc aaattgtgcc
    23041 agcagatatt tctcggcctg ttcattccag ataccgtgcg ttttctcaac cagccgcgcc
    23101 agctctttaa acagcggatc ggttttgcct ttttcggcaa aatcggcaat ggcggtcagc
    23161 ggcatggtca cgccgttata aatgagtttt ttaccgccag ggatatccgg cagattgagc
    23221 acggtttctg gcaccgcatc caggccaccg atatgggtca ccataaacga cggctgtaac
    23281 tgccccgtgg cgctaagggc aatcgcctct ttcatgtcgt ccgtggaacc gccggacgtg
    23341 ccgacaacgt gcgtactgtt gtaatggacg ttgtagaaat taaacggcac tttgaagttt
    23401 ttatctgtcg gcccggcgaa gaaattcaga caaccatcct ccgccagcaa ttcatccgcc
    23461 atttcaacaa cagcaggcac cgccgcataa acaaaaatgt catcgaaccc ggcatctcct
    23521 gtcagcgccc gcagcgtctg gaccggatcg ctcacccctt tggtattcat atacaccagc
    23581 tcaatgcctt tactggccgc cagttccacc ggcagcagct tttgtacctg cgtcaggcgt
    23641 ttgtcgtcga tatcgactac caccacccgc gacggttgta tgccgccgtt aatagcgtaa
    23701 tcgatagcgc caattcccat cgggcctgca cacgccagta gcgcaatatt gccgccaggt
    23761 ttgacgccca tgcgatgctc ataaacatat tgcgtggtgt gataattggc atgataagcg
    23821 ccaataatgc agcacatagg ctctgccagc gacgccgcag caaaataaga gccgtgatag
    23881 ggtaatacgc agcccaaatt aatggcgatt tcgggaataa tcatataggt ggcgttaccg
    23941 ccaaaatatt cgtagctgta gcccgctgaa tacccgcctg gtaaccccat cgccggttgc
    24001 aatacaaaac gctggccttt tttatatttg ccagtgagat ttttacccac ttcgacaata
    24061 accccggcac attcatgccc ggtaatgacc ggatgatttt ctaaatcgtc ggggacgcgt
    24121 ttatgttcac taccgagtaa cgccgctttc caggtggata aacaaacgct gtcagaaatt
    24181 acattcacta acagttcatt atcggtaatt tctggcagct caaattcgcg cagacggaca
    24241 tcccgcttgc cataaatagc agcaactttg gttttcattt ttacctctgt aatcagtgtt
    24301 tatgttcagt ggttaattga ttaaataaag tgtcgagttt tggatcgcca atatagttat
    24361 taatcaatat cagtggttta tccgtcacac gtttcacgcg cccttccaga ctggcgtgag
    24421 taacgacgat atccgcatcc gcaggcacgt tttctatggc gtaatgtttt acttcaattg
    24481 ccagccccgc tttttccaga cgtttacgga aggtggttgc gcccatcgcg ctggagccca
    24541 tccccgcatc gcaaacaaag gcgatacgct taacccggct taacgagaat gccccctctt
    24601 gtttcatcgc cttaaccgca ttggctgact gagcaaactc atcttcgctc tccgtttcca
    24661 ccgttttttc catcttcagt atcagcgaag tgatagcaaa ggacaccagg gtacctaccg
    24721 taacaccggc aattgtcgcc aggaacgagc ctttcggcgt cagtgccagg taagcaaaga
    24781 tagaccccgg acttgggcca gccaccagac caccgtccag taagttaaac atccaggtgc
    24841 cagacatacc gcccgcaatc atggcaataa tggtcagcgg cttcatcagc acatacggga
    24901 aatacagttc gtggatccca ccgaggaagt gaataatcat cgccccgggc gcagaacgtt
    24961 tactcatccc tttaccaaac aaggtaaacg ccagcagcag ccccagacca ggacctggat
    25021 tggaggccac catgaagaag atggatttac cgttaaccga agcctgttgc attcccagcg
    25081 gataatagac gccctgatcg atcgcattat taaggaaaag cactttcgcc ggttcattga
    25141 tgactgacag caaaggtaga taacccgcat gtaccagcgc ctcaatacac tctttgacga
    25201 aagtattagc aattaacacc gccgggccga taacttcaaa acccagcaga caaaggagca
    25261 tccccgcgat acctaatgag aagttattga taaccatctc aaaaccagca ggtatgcgtt
    25321 tttccagcga cttgtcgata tatttaatca ccagaccacc gagcggcccc ataatcattg
    25381 agccaaggaa catcgggatt tcagcgccga cgatcacacc aatcgtacca attccgccca
    25441 tgaccgcgcc gcgtttaccg ccgaccagat gaccaccggt agaaccaatc atcacgggca
    25501 ataaataggt aatcatcgga ccaacaattt tggcgaaatg ttcattcggc agccagccgg
    25561 tgggaataaa taatgccgtt ataaatcccc aggcaataaa agcaccgata ttggggatga
    25621 ccattgcagt caaaaagccc ccaaaagcct ggacctttgc acgagcagac ttgttttcca
    25681 taatattatc ctgtagagga gagagtaatt atccgcggct gataatgtcc gctaattgtt
    25741 tttctgatga tgcggttaag agttgttcga gtatttcttc ttcgcacagt aattcactta
    25801 acgcctgaat agcgccaatg tgcgaattgg catcggcagc agaaagcccg atgagtaatt
    25861 taatcggttc gtcattcccc ggaaaataaa cgctttgttt aagtaacgtc aaagacatcc
    25921 cggttttaag cgccccacat tccgatcgcg catgaggcat tgccacgcct ggtgcgagaa
    25981 tataataagg gccattgtta atggtggaac ctttaattgc ctgaatgtaa ttctcgctga
    26041 tatagttttt atccagcaat gataccatcg agaaatcgat agcttcctgc caatcttttg
    26101 ctgaatgtat aactgagatt gatgattttg gaaaataatc aataagccgc ataaattatc
    26161 cttattttat ttttagggta cagtccgacg gtaaaaataa aatacgcgta caaattaacg
    26221 aaagcaattg gacacatgaa aggagaaact ccacgagcaa aaataacaaa atattgaata
    26281 acaacaacat gaaataagct tttaaaaagt caattataaa tctgttaagt aaactacaaa
    26341 atcaacaaat tattcatcac tatatttgtg atacacatct ctattttctt attgaatgct
    26401 cgaaaacata cttattacgt ttattatttt ttcgctcatc ccctttataa attctgaatt
    26461 atcggctgtt gtctgttttc tcatcacctc atgtgacata ttccccactt tactcaagga
    26521 agattcatat gaaagcacct gtttcattaa cctcatttcg cccacaaaaa tctcttgcta
    26581 tcgccattgg cgtgcttgcc gtggttgtgc tgccatttct ctcgtattac accgttaatg
    26641 aaggtgaacg cggcattttg ctgcgctacg gaaaaatcgt aaaagttgcc gagccggggc
    26701 tgggttttaa gatccctttt atggaaagcg tagagaaaat ttccacgcgt aatcaggctg
    26761 tggtctatca gggattacag gcctacagcc gcgaccagca accggcacaa atgacagtat
    26821 cagtcagctt ccacattaaa ccttcagaag caggcgcggt ttataccacc tacaacacca
    26881 ttgaagcatt aaaagatcgt ctgattgtcc gtcagttgcc aactcaactg gagaacgttt
    26941 tcggacaata taccgccatc agcgccgtac aggatcgtac aaaactggtt caggatctgc
    27001 aaaatgccat gcgcaaagcc gttgttgggc cagtggtaat tgacggggtc cagattgaaa
    27061 atattgattt ttctgacgct tatgaaaagt cgattgaaga ccgaatgaaa gcagaagtcg
    27121 ccattgccac gcgtaagcaa aatctggaaa cggagaaaat ccaggcgcaa atagcggtaa
    27181 ctcaggccca ggcggaagca gacagtaaac tagcggcagc caaagcagaa gcggaaacta
    27241 ttcgcgtaag aggcgcggca gaggctgaaa ctattcgttt aaaaagtgcg gctgaagcag
    27301 aagctatccg cctgcgcggt gaagctctgc gggagaatcc cggacttgtt gccctcacca
    27361 ctgctgagcg ttgggacggt aaactgcccg ataccatgat ccccggcagc acagttccgt
    27421 ttatctc
//
LOCUS       DAOTRK010000059        26776 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.059, whole genome shotgun sequence.
ACCESSION   DAOTRK010000059 DAOTRK010000000
VERSION     DAOTRK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.059"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(6..2711)
                     /gene="secA"
                     /locus_tag="QIE41_002360"
     CDS             complement(6..2711)
                     /gene="secA"
                     /locus_tag="QIE41_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDS3531238.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(2773..3285)
                     /gene="secM"
                     /locus_tag="QIE41_002361"
     CDS             complement(2773..3285)
                     /gene="secM"
                     /locus_tag="QIE41_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDS3531239.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(3516..4433)
                     /gene="lpxC"
                     /locus_tag="QIE41_002362"
     CDS             complement(3516..4433)
                     /gene="lpxC"
                     /locus_tag="QIE41_002362"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDS3531240.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(4534..5685)
                     /gene="ftsZ"
                     /locus_tag="QIE41_002363"
     CDS             complement(4534..5685)
                     /gene="ftsZ"
                     /locus_tag="QIE41_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDS3531241.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(5746..7008)
                     /gene="ftsA"
                     /locus_tag="QIE41_002364"
     CDS             complement(5746..7008)
                     /gene="ftsA"
                     /locus_tag="QIE41_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDS3531242.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQILAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7005..7835)
                     /gene="ftsQ"
                     /locus_tag="QIE41_002365"
     CDS             complement(7005..7835)
                     /gene="ftsQ"
                     /locus_tag="QIE41_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDS3531243.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(7837..8757)
                     /gene="ddlB"
                     /locus_tag="QIE41_002366"
     CDS             complement(7837..8757)
                     /gene="ddlB"
                     /locus_tag="QIE41_002366"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDS3531244.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(8750..10225)
                     /gene="murC"
                     /locus_tag="QIE41_002367"
     CDS             complement(8750..10225)
                     /gene="murC"
                     /locus_tag="QIE41_002367"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDS3531245.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEAQHD"
     gene            complement(10279..11346)
                     /gene="murG"
                     /locus_tag="QIE41_002368"
     CDS             complement(10279..11346)
                     /gene="murG"
                     /locus_tag="QIE41_002368"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDS3531246.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAVKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQSQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11343..12587)
                     /gene="ftsW"
                     /locus_tag="QIE41_002369"
     CDS             complement(11343..12587)
                     /gene="ftsW"
                     /locus_tag="QIE41_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDS3531247.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(12587..13903)
                     /gene="murD"
                     /locus_tag="QIE41_002370"
     CDS             complement(12587..13903)
                     /gene="murD"
                     /locus_tag="QIE41_002370"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDS3531248.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(13906..14988)
                     /gene="mraY"
                     /locus_tag="QIE41_002371"
     CDS             complement(13906..14988)
                     /gene="mraY"
                     /locus_tag="QIE41_002371"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDS3531249.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGIIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(14982..16340)
                     /gene="murF"
                     /locus_tag="QIE41_002372"
     CDS             complement(14982..16340)
                     /gene="murF"
                     /locus_tag="QIE41_002372"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDS3531250.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALIARIKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16337..17824)
                     /gene="murE"
                     /locus_tag="QIE41_002373"
     CDS             complement(16337..17824)
                     /gene="murE"
                     /locus_tag="QIE41_002373"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDS3531251.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLAELLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCTGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(17811..19577)
                     /gene="ftsI"
                     /locus_tag="QIE41_002374"
     CDS             complement(17811..19577)
                     /gene="ftsI"
                     /locus_tag="QIE41_002374"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDS3531252.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(19593..19958)
                     /gene="ftsL"
                     /locus_tag="QIE41_002375"
     CDS             complement(19593..19958)
                     /gene="ftsL"
                     /locus_tag="QIE41_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDS3531253.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(19955..20896)
                     /gene="rsmH"
                     /locus_tag="QIE41_002376"
     CDS             complement(19955..20896)
                     /gene="rsmH"
                     /locus_tag="QIE41_002376"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDS3531254.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(20898..21356)
                     /gene="mraZ"
                     /locus_tag="QIE41_002377"
     CDS             complement(20898..21356)
                     /gene="mraZ"
                     /locus_tag="QIE41_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDS3531255.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(21958..22962)
                     /gene="cra"
                     /locus_tag="QIE41_002378"
     CDS             complement(21958..22962)
                     /gene="cra"
                     /locus_tag="QIE41_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDS3531256.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23044..23130)
                     /locus_tag="QIE41_002379"
     CDS             complement(23044..23130)
                     /locus_tag="QIE41_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDS3531257.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23142..23633)
                     /gene="ilvN"
                     /locus_tag="QIE41_002380"
     CDS             complement(23142..23633)
                     /gene="ilvN"
                     /locus_tag="QIE41_002380"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDS3531258.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(23636..25360)
                     /gene="ilvI"
                     /locus_tag="QIE41_002381"
     CDS             complement(23636..25360)
                     /gene="ilvI"
                     /locus_tag="QIE41_002381"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDS3531259.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQIAHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25480..25524
                     /locus_tag="QIE41_002382"
     CDS             25480..25524
                     /locus_tag="QIE41_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531260.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(25679..26623)
                     /gene="leuO"
                     /locus_tag="QIE41_002383"
     CDS             complement(25679..26623)
                     /gene="leuO"
                     /locus_tag="QIE41_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDS3531261.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6424 a         7602 c         6429 g         6321 t
ORIGIN      
        1 agcttttatt gcaggcgacc atggcactgc ttgtattttt taccagaacc gcacgggcaa
       61 ggatcgttac gtcctacttt gcgctcaccg gtttgcgccg ccagtgcagc tgcagctgca
      121 gagtcgtcat cctgatggct aagctgctgc atctgcgcta aacgctcggc ttccatacga
      181 cgctgttgtt ccagctcctc aacctcttca ggcatacgta cctgaacttt gctcagcgta
      241 ctgataactt catatttcaa cgactccagc atcgctgcaa acatggagaa cgattcacgt
      301 ttgtattcct gcttcggatc tttctgtgca tagccacgca ggtggatacc ctgacgcaga
      361 tagtccatcg ccgccaggtg ctctttccac agagagtcaa gcgtttgcag catgacgcct
      421 ttttcgaagt gacgcatcat ctctgcacca accacttctt ctttacgctg atacacttcg
      481 atggactgcg ccagaatgcg ctcacgcagc gtctcttcat gcagttctgg ttctttatcc
      541 agccactcgg caattggcaa atcaaggtcg aagtcgttct tcagacgttc ctgcagcccc
      601 ggaatatccc acatttcttc cagcgactgt ggcggaatgt aggcatcaat ggtcgctttg
      661 aacacatctt cacgaatgct gttgatggtt tcactcacat cgctgacatc cagcagttcg
      721 ttacgctggg agtaaatggc gcgacgctga tcgttagcca cgtcatcata ttccagcagt
      781 tgcttacgaa tgtcgaagtt acgactttca actttacgct gggcattggc aatcgcttta
      841 gtcacccacg ggtgttcaat ggcttcgcct ggcttcatac ccagtttacg catcatgccg
      901 gatactcggt cggaagcaaa aatacgcatc agcgcatctt ccatcgacag gtagaaacgg
      961 gaagaaccag catccccctg acgaccagaa cgaccgcgca actggttatc gatacgacgg
     1021 gattcgtgac gctcggtacc gatgatatgc aggccacctg ctgccagtac cgcatcgtga
     1081 cgtacctgcc agtcggcttt aattttttca atttgctctg cggtcggatt ttccagcgcg
     1141 gcaacttctg cctgccagct accaccgagc acaatatctg taccacgacc cgccatgttg
     1201 gtcgcgatag tcaccgcagc cggataacct gcctgagcaa caatcgccgc ttcgttggcg
     1261 tggaatttgg cgttcaggac gttgtgctta ataccggctt tggtcagttc gtttgatacc
     1321 agctccgatt tttcgatgga gatagtaccc accagcaccg gctggccttt cgcagtacgt
     1381 tctttgatat cttcaatgat cgcctgaatt ttttccgctt cagtcatgta gaccaggtcc
     1441 ggcagatctt tacgaatcat tggacggttg gtcggaacaa cgacggtatc cagcttgtag
     1501 atggagctga attcgaaagc ttcggtatca gcagtaccgg tcatccccgc cagtttttca
     1561 tacagacgga agtagttctg gaaggtgatc gaagccagcg tttggttttc gttctggatc
     1621 tgcacacctt ctttcgcttc cacagcctgg tgcagaccat cggaccagcg acgcccctgc
     1681 atggtacgac cggtgtgttc gtcaacgatg ataacttcac catctttaac gatgtaatcg
     1741 acgtcgcggg taaacagcgc atgagcgcgc agcgccgccg ttacgtggtg catcagcatg
     1801 atgtttgccg gagagtacag agactcccct tcatccatga tgccttcttt caccagcagt
     1861 tcttcaatca ggaccagacc acgttcggtc aggttcacct gacgagattt ttcgtccacc
     1921 gagaagtggc cttcgccctg gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc
     1981 ggaataattt tattcacgcg tttatacatt tccgagctgt cttctgccgg gccggaaatg
     2041 atcagcggtg tacgagcttc atcgatcagg atggagtcca cttcgtccac cagcgcatag
     2101 tgcagtttac gctgtacacg ttcttcaggg ctgaacgcca tgttatcgcg caggtagtca
     2161 aagccgtatt cgttgttcgt accgtaagtg atgtcagctg cgtaagcttc acgcttcgcc
     2221 ggtgctggca tgcccggcag gttgataccg acagtcaggc caaggaattc aaacagcgga
     2281 cggttgtttt cggcgtcacg ttgcgccagg tagtcgttga cggtaacaac gtgtacgccc
     2341 ttaccggtta atgcgttcag gtaagcaggc agcgttgcgg tcagggtttt accttcaccg
     2401 gtacgcattt cggcgatgca gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg
     2461 aagtgacgca taccaaagac gcgcttactt gcctcacgca ccacggcgaa agcttccggg
     2521 atcagatttt ccagcacttc gcctttttcc agacgtgcac gaaactctgc ggttttccct
     2581 ttcagttctt cgtcggagag tttttccatc tccggttcca tggcattgat gatgttaacc
     2641 actttgcgca tccggcgcag ggtgcgatcg ttacgactac cgaaaacttt agttaataat
     2701 ttgattagca taataaaatc tcaaacgccc cgcgttgcgg agtcaataaa atgaagtaaa
     2761 ggtttattgt tgttagctga ggcgttgagg gccagcacgg atgccttgcg cctggcttat
     2821 ccagacgggc gtgctgaatt ttgcttgcgg ggtaaaatgc gcataatcaa tgcgataacc
     2881 cttttcagac ggcgtgcctt cctgggtcaa cagcgcgctg agcgtatcca gtaatgcaag
     2941 atgttgcgcc tgaagaggca aagattcttc agcaacgggc agcgtttgcg gtgccattgc
     3001 gaaggaaaga tgacggatta ccgtgcgaat ggcatgttga tgccagtaat caacggaata
     3061 gttcgaattc gggcggcgcg tgttcgcttc cagcaaggcc aattgaccaa agttaacttt
     3121 ggctgaaggc tcgtggttgc gggttgtcgc ttttgcgggc gcgtttggtt cggcggcgtt
     3181 gctgagcgca ggcaaaccta aactcgccgc gaccatccct aataagagat gcggccagaa
     3241 gtagcgttta ccaaactgtc gccagcgcgt cagtattcca ctcacgttat tgccatcccg
     3301 atcgtattca aacgtattta gcgcacaaat cttatcatta aagcctgaag tccacagcac
     3361 gaatgaagat aagttgtgcg taaaagtgct taagaaaaca gcgttcgcac cgttttttat
     3421 cgttcttata aaattcaaaa aaaaacgact ggttcacctg gccggagaga gtgccagatt
     3481 tgccagtcga attttatacg acagtataaa tgtcgttatg ccagtacggc tgaaggcgct
     3541 ttgaaggcca acggcagttc tgcgtcgtcc tggaaggtca catattccca ggcttcctgt
     3601 tttgcaagga cagcctgcag cagtttgtta ttcagtgcat gaccagattt ataagcggta
     3661 aatgcaccaa taatattgtg accacacatg aacaagtcac cgatcgcatc gagcattttg
     3721 tgacgcacaa attcgtcttc aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca
     3781 acaacgatgg cacaatcgaa gctgccgccc aggcacaaac cacgggactg cagatattcg
     3841 atatcacgca tgaaaccgaa cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg
     3901 gagaagttca tcgcatagcg ctggttgctg gaatcaatag ccggatggtt aaaatcgatg
     3961 gtgaaatcca gcgaaaaacc attgtacggc ttaaattcag cccacttatc gccatcttcg
     4021 acacgaacag tctctttgat gcgaacaaat ttcttggcgc agttcagctc gtcgataccg
     4081 gcgtcaagca gcaggtatac aaacggagcg gcgctgccgt ccatgatcgg gatttccggc
     4141 gcgttaactt cgataacaat gttatcgatg cccaagcccg cgagagcagc attgaggtgc
     4201 tctacggttg aaatccgtac atcatgctcg ttgaccagac acgtacagag catggtatca
     4261 cgcacagatt tggcatcggc cgggaaatct accggtggat tcaagtcggt gcgacgatag
     4321 atgaccccgg tgttggccgg cgcagggcgt aacgtcaggg tgactttctt gccggtatgt
     4381 aaaccgacac ccgtcgcctg aacgatacgt ttaagtgtcc tttgtttgat catcgtatta
     4441 tctcgccaaa ttacctatcc aaccgaagtg tactatacat tcggcgggcc agtttagcac
     4501 aaagagcctc gaaacccaaa ttccagtcaa ttcttaatca gcttgcttac gcaggaatgc
     4561 tgggatatcc agataatctg gctctttcgc agtttgcggc gcattgtcat tcacgacttt
     4621 agcaaccggc ttctgctcct gggtcagcgg agccatcccg tgctgctggt agcgatccat
     4681 cactggctgc tgaacctgct tattggtcac cagagtgatt tcaggacgtt tgtccatgcc
     4741 gatacctgtc gcaacaacag ttacgcgcag ctcgtcattc atatccgggt caagagaagt
     4801 accgataacc acagtcgcgt tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc
     4861 gaactcatcc agacgcaggt cgaagcccgc cgtgatgtta accagcacgc cgcgtgcgcc
     4921 agacaggtcg atatcttcaa gcagcggaga agagatagcc atttcagcag cttcttccgc
     4981 acggtcttca ccgctcgcca cgccagaacc catcattgcg tagcccattt cagacattac
     5041 ggtgcgtacg tctgcaaagt ccacgttcat caaacccgga cgagtaatca gttcggcgat
     5101 accttgcaca gcgcctttga gtacatcgtt cgctgcgcca aacgcatcca gcagggagat
     5161 accgcggccc agaactttca gcagtttgtc gttcgggata gtgatcagag agtccacatg
     5221 tttggacagt tcagtgatcc cctgctccgc gaatgccata cgcttcttgc cttcaaagtt
     5281 gaaaggctta gtgacgacag caacggtcag gatacccaaa tcttttgcca cttcagcgac
     5341 gactggagct gcgcctgtac cggtaccacc acccataccc gcagcaataa agaccatgtc
     5401 tgcaccttcc agcgccgcac gcagtgcatc gcgatcctca tcagccgcat tacggccaac
     5461 ttctggatta gcgccagcgc ccagtccttt ggtgataccg ctaccgattt gaatcgtctg
     5521 tccaaccgct gttttacgca gcgcttgtgc atcggtattt accgcgaaga attcaacacc
     5581 ttcaatgcgc tcgcgcacca tgtgttcaac agcattaccg ccgccgccgc cgacgccgat
     5641 gactttaatc accgcgtcat tggtaagttc cattggttca aacatagttt ctctccgatt
     5701 tgtgcctgtc gcctgaggcc gtaatcatcg tcggcctcat aaaaattaaa actcttttcg
     5761 cagccaacta ttgagtcgct tgatccacga gccaactgat gctgtaacac gtttttctac
     5821 ttcagcttca ccgttaagat gtgactcttt tccatagtga agcaatccca ccgccgtcga
     5881 ataatacggc tcctgagcat aatccgttaa accggtaatg ttcagcggcg cgccgatacg
     5941 cacttgcgta tgaaacacgc gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc
     6001 accagttaat acaatgcctg ccgccaggtg atgtttaacc ccttgttggc gaagcttttc
     6061 ctgcaactgc aatatctctt cgttgaccag gttgagcagc tcggtatagc gcggctcgat
     6121 cacctctgcc aggatctgac gttgcagact ccgcggcgga cgaccgccta cgctcggcac
     6181 ttccacgctc tcatcttttc caacgatgga acccagcgca caaccgtggc gaactttaat
     6241 cgcttcggcg tcgcttggcg gcgtgccaaa ggcgtaagcg atatcactgg tcacgacatt
     6301 gccagcataa ggaattacct tagtgtggcg caatgcccca ccggtataaa cggcgatatc
     6361 cattgtacca ccaccgatat cgacgacgca gacacccagt tcacgttcat cttccgtcaa
     6421 taccgaataa cttgatgcca gtccggcaaa tatcagttgg tcaactttca gcccacaacg
     6481 ttcaaccgct ttgacgatgt ttttcgccat atcgttgtga catgtgatca ggtgcacttt
     6541 tgcctgcatc cgcacgcccg aaagtcctac cggattcttg atcccttcct gatagtcaat
     6601 cgcatactct tgcgggatca catgcagcac acgatgctca tcgcgcacac gcaccgattt
     6661 cgcggtatgg acgacgtttt ccacatcttc ttgcgtcact tcttcttcag aaataggcac
     6721 cataccaatt tcattctggc agctgatgtg cttaccagaa agcgccagat ataccgaaga
     6781 gatctgacaa tctgccatca attctgcctg gtcaatggca cgttgtacgc acttgaccac
     6841 ggattcgagg tcgttcaccc cgcctttatc cataccacgt gacgggcagc tgcccacgcc
     6901 aatgatattg accataccgt cgggcagaac ttcccctact aaagcggcaa ccttcgcggt
     6961 accaatctcc agtcctacta ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc
     7021 tgtgcctgat tttgttgctg agtagattcc tctggcggca agggcgccca gcctactgcc
     7081 gctccagagt cataacgcaa atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc
     7141 tgctgtaaaa ccggataaag ttctacaaag cgagccaaac gtttcatcgt atcgccccgg
     7201 ccaagattga gcttaatatc gttattcagc gtcaactgcc aggaacgccg cgcggtcatc
     7261 gccgcttcct tcagagtaaa tctgtccttt gccagcatct gccccatttc gcgatagccc
     7321 tgcaacactt cattagcgct gccttccggg ccatacagca ttggaagcac ctgcttgctg
     7381 gtgcgatctg gcggcacgct gaaggtgttt ccttccgcgt ctaccatgtg ttgatcattc
     7441 caccgcgcaa tcggcacata ttcaaccaga tgaatcttca attcatcagg ccactgcttt
     7501 ctgacgctca cctgcttaat ccacggcagg cgttgttcta tttgcgtctg gatgatgttg
     7561 acatcctggg tcataaaggt acccggctca cccaatgcca ggatcgactg ccggatatcg
     7621 tcattacgcg tgtaatggcg ttcaccggtc aacaccagct ttgagagcgg caggcgttgc
     7681 gcatcttcca tccagcccaa cacgacccag ccgctcacca acactgtcgt taaaacggtc
     7741 agcaggaaaa ggatccccgc cagacgcgtt ccattattgc ggcgagaaga aacctcttct
     7801 tcgctgtttc gcgtgttcag agcagcctgc gacatattag tccgccagtt ccagaattcg
     7861 tactaccaac tgcgagaagc tcatacctgc ctgacgtgcc gccatcggca ccaggctgtg
     7921 gctggtcata cccggtgagg tattggcttc cagcagataa aactgtccat cgctgtccag
     7981 cataacgtca atacgtcccc atcctttgca acctaacgtc gtccatgctt tcagcactaa
     8041 tgcctgcaaa ttggcctctt gtgacgcttc cagacctgcg gggcagaaat actgtgtctc
     8101 atcagagaga tacttcgcct cataatcata gaaggttccg gacggttgaa tacgtattga
     8161 cggtaaaatt tcttcaccga gtatcgcaac cgtgaactcc ggcccactta gccatttttc
     8221 aatcaatact tcttcatcgt gctgaaatgc caatcttaat gcatcttgta gagcattttc
     8281 tgctactact tttgacattc ccacactgga accttcgcgg ctcggcttaa cgataaccgg
     8341 caaacctaag gcagaaattt ctgctaactg cttatcactc aggccttttt caaactctgc
     8401 gcgggttaac gctacccacg gagcgaccgg taaaccggca ccttgccaca gaagtttgct
     8461 gcgtagttta tccattgaaa gcgcagatgc catcactccg ctgccggtat aaggcaagcc
     8521 catcagctcg agcatgccct gcagcgtgcc atcttcaccg ccgcgaccgt gtagcgcgat
     8581 aaacactttc tgaaagccca tcgacttcag ttgcgtcacg tcgacttctt tcgggtcgac
     8641 aggatacgcg tcaataccgc cttcacgcag cccggctaac accgctgcgc cagaattcag
     8701 agaaacttcc cgctcagcgg aggtcccacc caacaggacc gcgattttat cagtcatgtt
     8761 gtgcttcctc cggagtttgc ggcttcagtt tgatttcagc taaagaacgg gcaatttttc
     8821 caatattacc agccccctga acgagaatca ggtcgttacc ggttaatacc ggtgccagca
     8881 tctcggctac ctgcgccggg tcaggcacca gaatgggatc aattttccca cgtccacgaa
     8941 ttgtgcgaca cagcgaacgg ctgtccgctc ccggaattgg cgcttcgcca gccggataca
     9001 cttccagcat caacagggta tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt
     9061 cgcgtgtacg ggtaaaacgg tgcggctgaa acagcattac caggttttta tccggccagc
     9121 ctgcacgcgc cgctttaata gtggcgtcca cttccgtcgg gtggtggccg tagtcatcga
     9181 ccaacattgc cgtaccgctt ttaccattca ctggctccag cgggaattca ccgaggaaat
     9241 caaaacgacg accagtaccc tggaagctct ccagcgcacg caaaatagcc tcgtcgtcaa
     9301 ttccctcttc cgtagcaacc gcaaccgcag ctgcggcgtt cagcgcgtta tgacggcctg
     9361 gcgcattcag ggtgacgcgc atcggctctt tgtcctggcg cagcagcgta aagtgcccct
     9421 gcgggccaat ctgctgataa tcttctacac gcacgtcggc atcttcgctg aagccgtaag
     9481 tcgtggtctg acgtcccaca cgcggtaaca attcgcggat caccggatca tcaacacaca
     9541 tcactgcacg accgtaaaac ggcaggttgt gcagaaaatt aataaaggtc tgttttaaat
     9601 tctcaaagtc gccctggtag gtatccatgt ggtcggcttc gatattggtg acaatcgcca
     9661 ccatcggttg cagatgcagg aacgatgcat cactctcatc tgcttcggca atcaggtacc
     9721 gaccatgccc caaacgcgca tgaacgcccg ccgcttttac cagcccaccg ttaacgaagg
     9781 ttgggtcgag tcccgcttct gcgtagatgc tggaaaccat cgcagtggtt gtcgttttgc
     9841 cgtgcgttcc ggcaatggcg atgccatgac gaaaacgcat taactcagcc agcatttcgg
     9901 cacgacggat caccggaata cgcgcttcat gagctgcgac aatttccggg ttatcggcag
     9961 aaatcgcgct ggaaacaacg accacgctgg catcacgtac gttttccggg cgatggttga
    10021 aataaatcgt cgccccaaga ttcattaact gctgcgtgac cggatttggc gctaaatcgg
    10081 aaccactgat ctgataacct tcattggcca gaacttcggc aataccgccc ataccggcac
    10141 caccgatgcc gacaaaatgt atgtgccgaa cgcgacgcat ttcgggcacg atggaacgca
    10201 gttttgccaa ttgttgtgta ttcattcttt acgccattaa cttcttaaat tcatacgatg
    10261 caaaaggcat cgctacaatt acgcccgggc agcccggctc acttcatttg ccactcgctc
    10321 ggtggcatcc ggaatggatg cagcgcgggc gcgttctgcc atggttaata aggtttctcg
    10381 cgaccacccg gccagggtgt tggcgacagc atccacgcta agctgtggct gctcgataat
    10441 tttggctgcg cccgcttttt ccagcggtag agcattccag tattgctggc ggtctttatg
    10501 ttgaaacggc acaaacaacg ccggtagtcc tgccgcagcg atttcactca ccgttaacgc
    10561 accggagcgg caaacgacga catccgccca cgcatacgcc gccgccatat catcaataaa
    10621 ttccgtcact ttatgctgcg attgccccgc ttcggcatac gcctgttcaa cggattgttg
    10681 cgaacctttg ccgctctgat gccagatagt gactgaatca cccagtttca cagcaacctg
    10741 cggcattgtc tggttaagaa tgcgtgcgcc ctgggaacca cccactacca acacacgaac
    10801 cggaccttca cgtccagcca aacgttgctg cggcaacggc agcgccagca catcggtacg
    10861 caccgggtta cccaccactt ccgcattagg gaaagcgcct ggaaacgcct gcatcacttt
    10921 ggtggcaatc ttcgccagcc atttattggt taagcccgca ataccgtttt gttcatgaag
    10981 tacaaccgga atgcctaacg accacgcggc cagaccacct gggcctgata cgtagccgcc
    11041 cattccgagc accacgtcag gtttgtacgc tttcataatc gcccgcgcct gacgccaggc
    11101 gttgaagatt cgcagtggcg cagctatcag tgcttttata ccttttccac gcagaccaga
    11161 gatacgaatg aaatcaattt cgatgccatg ttttggcact aagtccgctt ccatacggtc
    11221 ggcagtcccc agccagcgaa cttgccaacc ctgagccatt agatggtgcg caaccgccag
    11281 tcccgggaat acatgtccac cggttccgcc tgccatcacc attaatcgct ttccttgacc
    11341 actcatcgtg aacctcgtac aaacgcctgc gctttctcca gacgcgtttc ataatcaata
    11401 cgcaacagca tcatgatggc tgtcgacata atcagtaagc tcgaaccacc gtaactgatc
    11461 agtggcaatg tcagaccttt ggtcggtaac atccccgccg ccgcgcctac gttaaccagc
    11521 gcctggaagc taaaccagat gccaatggaa caggcgagaa aaccggaaaa acgatggtca
    11581 atttctaatg ctttacggcc aatcgacatc gcacgaaaag cgacgaagaa taccattaaa
    11641 agtgctagta ccacaccgac ataacctaat tcttcgccga taatggcgaa aataaagtca
    11701 gtgtgcgctt ccggcagata ctccagtttt tgtaccgagt tacctaagcc ttgcccccaa
    11761 agttcgccgc gaccaaacgc catcagcgat tgcgttaact gatagccgct gccaaaggga
    11821 tcttcccacg ggttccagaa tgcggtaaca cggcggatac ggtacggttc ggcgaggatc
    11881 agcaacacaa ccgctgaaat gcccataccg ataatggcaa tgaactgcca caatttcgct
    11941 cccgccagga ataacatcgc cagcgtagtc acaaacaaca ccaccaccgt accaaggtct
    12001 ggctgtgcca gcagtaacac tgccaacacc agaatcacgc ccatcggttt caggaagccg
    12061 cgcaggttat tacgtacttc gtcgccttta cgcaccagat agttggcgat atagcaaaac
    12121 aacgacagtt ttgtcagctc cgcaggctgg atacgcagca agccgagatc gatccaacgc
    12181 gatgcccctt taacagagct acccactacc agaacgatca tcaacaggat gattgatccg
    12241 agtagcatcg tagcgctgta acgttgccag aactccatcg gcagacgcag cgtaatgatc
    12301 gccagaatga atgccaggat cagatagacg ccatcacgct tcgcgaagaa gaacggatcg
    12361 ttggttaagc gttgcccgat gggcattgac gccgaggtca ccatgataaa gccaatcgcc
    12421 gcgaggccga aggtcagcca cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct
    12481 ttttcccgcg agcccatcac ccagcccttt agcgccgtgg agatccagac cagaatactg
    12541 aatcctggca ggcgcggcat tttcaggcga gggagagata aacgcatcaa cctaactcct
    12601 tcgccagacg ggcaaactca ttgcctcgtt gttcaaagtt cttgaactga tcaaggctgg
    12661 cacaggccgg ggagagcaga accatatcgc ccggctgaac acgcggagcc agcaagcgca
    12721 tcgcctgttc catggtttcg gtttgttctg ccacttccgg gcgtagcgcc gccagctgcg
    12781 cgccgtcccg accgaaacaa tacagacgta cgttatcgcc attcaggtaa cgcgccagtg
    12841 ggctaaaatc tgccgattta ccatcgccgc ccagcaacaa atgcagcgtg ccgtctacgt
    12901 gcaggccatt cagtgccgct tccgtactgc cgacgttggt cgcttttgaa tcgttaatcc
    12961 agcgcacacc gttatgctcc agcacaactt caaagcgatg cggcagacca gtgaatgtgg
    13021 ttaacgcttt caggctgctg gcgcgcggca aaccagcggc atctgccagt gccagagctg
    13081 ccagtgcatt ggtgtagtta tgctgcccgg aaagtttcat ttctttcacg ttcagcactt
    13141 tctcgccctt cacccgcagc caggtttcac cctgctgatg attcaggtga tagtcaccca
    13201 tgttgacgcc aaagctgacg cagcgttcat ccgcaccgcg aatcggcatt gttaaggcat
    13261 catcagcatt aaccacgcaa actttcgcgt tttcgtaaat gcgcagtttt gctgcacgat
    13321 actgttgtaa accaaacgga tagcgatcca tatgatcttc agtcacgttc agaatggtcg
    13381 ctgctaccgc ctgtaaactg gaggtggttt caagctggaa gctcgacagc tccagcacgt
    13441 acagttcgca ctcatcatcc agcagcatca acgcaggcag gccaatattg ccacccacac
    13501 caacgttaac ccccgccgct ttcgccattt cacccactag cgtggtgacc gtgcttttgc
    13561 cgttagaacc ggtgatcgcc acaatcggtg cttgtgcttc acgacagaac agctcgatat
    13621 cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa ggatggatgc gccagtgcaa
    13681 taccgggact ggcgacaatc agatcggccg ccatcagcca ttcatcattc agaccgcccg
    13741 tgtggcgttc tacagcttcg ggtaatttat ccaggccagg cggtgtcata cgcgtatcca
    13801 taacgcgcgg cgtcacaccg cgagcgagga aaaagtccac gcaagaaagc ccggtgaggc
    13861 ccaggccgat aatgacgaca tttttaccct ggtaatcagc catgattaac gtaccttcag
    13921 cgttgccaga ccaatcagga ccagcatcag cgaaataatc cagaaacgta caatgacgcg
    13981 cggttccggc cagcctttca gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg
    14041 ttgtccgcgc agtttaaagg agccgacctg caggatgacg gaaagcgttt ctaccacgaa
    14101 cacgcccccc ataatcacca gcaggaattc ctgacgcagc agtacggcga taatacctaa
    14161 cgcaccacct aacgccagcg aaccgacatc gcccataaag acctgcgccg gataggtgtt
    14221 aaaccacagg aagcccagcc ctgccccgac tatcgcggta cagacaatga ccagttcccc
    14281 ggcgtgtcgt agatacggta tgtgtaaata gctggcaaag ttcatgttgc cggtcgccca
    14341 cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc ataattgcca gaccgtcgag
    14401 accatcagtc aggtttaccg cgttgcctgt acccacaatg acgaagtaag ccagcagaat
    14461 gtagaacagc cccagttgcg gcatcacatc tttaaagaac ggaaccacca gctgcgttgc
    14521 gggcgtgtct ttgccggcaa ggtacagcgc gaaggcaaca cccagcgcaa tgaccgacat
    14581 ccagaaatac ttccaacgag cgatcaaccc tttggtgtct ttacgcacca ctttgcgata
    14641 gtcatcaaca aagccaatga tgccgtaacc taccagcacc accagtacgc accagacgta
    14701 cggattggat ggataagccc acagcagcac ggagatcaca atcgctgtca ggatcataat
    14761 cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat tcaggaccgt cgttacgcac
    14821 tacctgacca aaggaaagtt tttgcaaatg agcaatcata cgcgggccca tccacaatga
    14881 gatgaacagc gcggtcagca ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt
    14941 aaagccggaa taatatttga ccaaatgttc ggccagccaa actaacatgt cccattctcc
    15001 tgtaaagcgc gtactacctc ttccatggcg gcactacgtg aacccttaac taaaatcgta
    15061 attacctgtt gctcagcaat cagtgattta atacgcgcga taagcgctgt tttatctgca
    15121 aaatgttcgc caacgccgct ggcggtgctg atagcatggc tttgtttacc cacgcttaac
    15181 acgcggtcaa taccagcagc ttttgccgcc tcgcccacct gtacatggca ggcttcgctt
    15241 tcagcgccca attccgccat atcgcccacc accagcacgc ggtagcccgg catttcagcc
    15301 agtacctgga ctgctgcggt cattgaaccg acattggcgt tgtaggagtc gtcgagcagc
    15361 aactggtttt ccgccagttg gatggggaac agacggcctg gaacagcttt cagatttgcc
    15421 agccccgctt tgatagcatc aagcgttgcg cccacggaca tggagagcgc agcggctgcc
    15481 agcgcattcg caatattgtg acgccctgga agcggcagca gaacatcgac gctacctgtt
    15541 ggggtttgga gggtaaattc cgtaccgtgc gaagtcacat ggatgttggt ggcggtgaaa
    15601 tcgctgttgg cggcattcgg tgagaaacgc cacactttgc gtgagccaat tacgctctgc
    15661 cagttcagcc agtcgttgtt gtcggcattc ataatggcga taccgttttc cggcaggccg
    15721 ctaaagattt cgcctttcgc ttttgccacg ccagccaaag aaccgaaacc ttccagatgc
    15781 gctgccgcca ggttattgac cagcgccgct tccgggcgag tcagactcac ggtccaggca
    15841 atttcgccct gatggttcgc gccaagttca ataactgcgt aatcgtattc cggcgttaag
    15901 cgcagcagcg tcatcggcac gccgatgtcg ttgttgagat tgcctgccgt ataaagcgtg
    15961 ttgccgcact ggcttaaaat tgccgccgtc atctctttaa cagaggtttt gccggaggag
    16021 cccgtcagag caaccacgcg cgccggaact tgctggcgaa cccatgcagc cagttcacca
    16081 aacgccagac gcgtatcctt gacgattaac tgcggcagat cgatatccag cggacggctg
    16141 accagtagtg cgcccgcgcc gccagctttc gcctggtcgg caaaatcatg agcatcgaaa
    16201 cgttcgcctt tcagggcaac aaacaggcag cccggcgtca gttttcgcgt gtcagtggtt
    16261 acagcatcaa gggtgatatc tgcaccttgc agttcaccgt tgagaatatc ggtaagttgg
    16321 ctaagggtta cgctaatcat gcaatcaccc ccagcagacg cgccaccgtg acgcgatcgg
    16381 agtagtccag acgctgattg ccaacaatct ggtaatcctc atggcctttg cccgcgacca
    16441 gtaccacatc attctcttta gcctgcataa cggcgcaagt caccgcttca gcacggcctt
    16501 ccatcacttt ggcatgtccg gcatctaaca ttcccgccag aatatcgttg atgatggcac
    16561 gcggttcttc ggtacgcggg ttatcgtccg ttaccaccgc cacgtcagcg aactcttcag
    16621 caattgcgcc catcagtgga cgtttgcctt tatcacgatc gccaccacag ccaaagacgc
    16681 accacagctt gcccgtacag tgcaggcgcg ccgcctgtaa ggctttttcc agtgcatctg
    16741 gcgtatgcgc gtaatccacc accaccgtcg gtttgcctgg tgcagtgaac acttccatac
    16801 gtccgcaaac cggttgcaga cgcgcggcgg ttttcagcag ctcagccagt ggatagccca
    16861 gcgccagcag cgtcgccagc gcgagcagca ggttgctgac gttaaaagcg cccatcagat
    16921 ggctttcaat ttcgccatcg ccccaacttg agctaaagcg aatcgtcgca ccgctgtcgt
    16981 gatagttcac gtcgatcgct tttaaccagc gtccgtgaca attcggatta atatgatctt
    17041 ccattgatac cgcaaccgcg tccggcagtt ttgccagcca gcggcggccc acttcatcgt
    17101 cggcgttaat aatcgcctga ccgcaatgat gttcagaata aagcagccat ttcgcagctt
    17161 cgtagtgttc catatcacca tgataatcaa ggtgatcgcg gcttaagttg gtaaagaccg
    17221 acgccgcaaa ttttaatgcc gccacacggt gctgtaccag cccgtgggag gaaacttcca
    17281 ttgcgcaaaa agtcgcgccc tgatccacca gccccgccag ctcatgctga acatcgactg
    17341 ccgaaccggt ggtattttct gtcgggatca ctttccccag cagaccgtta ccaacggtgc
    17401 ccattaccgc gctggtttcg ccaagcagtt ggctccactg cgccagcagc tgggtcgtcg
    17461 tggttttgcc gttggtgccc gttacgccta caagacgtaa attgtcagag ggttcatggt
    17521 aaaagcggcc cgccagtgca gataaacgct cgttgagctg gctgagatag atgaccggta
    17581 cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcatc tttcgcctct gcaataatgg
    17641 cagccacacc ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc tgatgaccta
    17701 ctacagctac aaagagatcg cccgccgcag ccacacggct gtcgagtgtc atctctcgca
    17761 gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg
    17821 ccacctgtcc cctcgccttg attaatcaca aattcatttt tatcgcccgt tgtcagcgca
    17881 tccggctcga tgttcatggt acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg
    17941 gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt tgataacaac aaccagcgcg
    18001 aagcgcggct gactcgcagg cgcaacgcct gcggtataag caatatattt attgatgtag
    18061 cgaccgtccg gcccaacctt tttcgcggta ccggttttaa tggcgatacg atagccctta
    18121 atcgccgcct tcacgccgcc accacccggt agcgccacgc tttccatcat atgcaccacg
    18181 gtgcgaacaa tggattccgg gaagactcgt tcaccgggaa ccggggggtc aactttggta
    18241 atcgacagtg ggcgataaat gccgtagctg ccgatagttg cgtagactcg cgctaactgt
    18301 aacggtgtta ccattagccc gtagccgaaa gagaaggtgg ccctctctat gtcagaccac
    18361 cgttgttttt gaggatataa gccactgcgt tctccgacca accccaaatt ggtcgctttt
    18421 cccagtccaa aacgtgagta agtatctact aacgctgagg acggcatcgc taacgccagc
    18481 ttggaaacac cgacgttact cgacttctgt aataccccgg tcagggttaa ttcgctgtag
    18541 cgtgccacgt ctttgatttc atggccgtta attcgataag gaacggtatt cagtaccgag
    18601 ttttcccgca ctacgccacg ttgcaacgcg gtcatcacca ccatcggttt aaccgttgaa
    18661 cccggttcaa acacgtcagt gatggtacgg ttacgcatcg cctctttcgg cgtgccactc
    18721 agattgtttg ggttgtatga cgggctgtta gccatcgcca gcacttcacc ggtgttgaca
    18781 tccaccagca cggcgctacc agattcagct ttgttaaagg ccaccgcgtt gttcagttcg
    18841 cgataaacca gcgcctgcag gcgttcatca atactcagcg ccaggttgtg cgccgcctga
    18901 ctgtcggtgg aagagatatc ttcaattacg cgaccatagc ggtctttacg cacaatgcgc
    18961 tcacccggct gcccggtaag ccatttatcg aaactcttct cgacgccctc aatcccctga
    19021 ctatcgacgt tagtaaagcc gatgaggtga gcggtcactt cgccggatgg ataataacgg
    19081 cgagattctt cacgcagatg aatccccggc agtttcagtt ttttgatgta ctctgccatg
    19141 tcagggttta cctgacgcgc cagataaata aagcgccctt tcgggttggc gttaatgcgg
    19201 gctgaaagct gatccagcgg aatattgagt gcgttagcca gcgccttcca gcgatcaccg
    19261 acgctgatac cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc ttttaccggc
    19321 acgctcaccg ctaacgggcg accagaacgg tcagtaatca tgccgcggga ggtggaaact
    19381 tgctgaacgc gaagagaacg catgtcgccc tcttttacca gcatatccgg agagataact
    19441 tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca gccgcataac
    19501 aacgcaaaac gccaactgat aaagttggca tgttcttcct gacgttttgg tttctgcgtt
    19561 ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt gcactacgat attttcttgt
    19621 gacggatcaa catgctgcat ttgcagcttt tccgtggcga tcctttccac ccggctatgg
    19681 tcgccgagcg cattctcttc aaggatcagg ttgcgccatt caatgtctaa agcatctcgc
    19741 tccagcacca gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc
    19801 acggtcaccg ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc
    19861 aaaagatcgt caccgataac accaggcaat gcatggcgct cgtggcttcc catcgatcct
    19921 ttaactttgc ttagagcttc tgtcactctg ctgatcatgc gttcgtcctc tctgcaatac
    19981 gcagaactga actacgggca cgagggttct cagccacctc ttcttcgccc ggcattaact
    20041 tgcctagtgc tcgcagctga cggccaccca gttttttgag ctgctcttca gtcatcggta
    20101 accctgccgg aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc acaatacggt
    20161 cttccagcga gtggaagctg atgatcgaaa gccgcccacc cggggccagc acgttgagcg
    20221 agctttttag cgcctgctct atctcctcca gttcactgtt tacccaaatg cgcaccgcct
    20281 ggaaggtccg ggtcgcggga tgtttaaact tatctttcac cggcgtagcg gcagccacga
    20341 cttccgccag ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc
    20401 gggcaatgcg tttggcaaaa cgctcttcac cataggtttt caatacccag gcgatatcgg
    20461 cttcttctgc ggtttgtagc cattcagcgg ctgactgccc acgggttggg tccatacgca
    20521 tgtccagcgg accatcgcgc ataaaggaaa agccgcgttc agcatcatca agttgcggtg
    20581 aagagacgcc aagatcgagg agaatgccgt cgatcttgcc gataagatcg cgctcggcaa
    20641 catattcgcc cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag
    20701 tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc
    20761 caagctgcga gaggatcaga cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga
    20821 tgccatcagg acggatattg aggccattaa cggcttcatc cagcagcacc gtagtatgtt
    20881 tatagttttc catcatttta tagagacaag tcctgcagtc gctctgataa gtctccagta
    20941 gccaactgct ctgcgtcgat atcttccttg acctgttgat gccaggttgt ttcatcccac
    21001 agctcaaact tgttgaactg tccaaccagc atcacttctt ttgtcagccc ggcatgttgc
    21061 cgcagtactg gcgcgattaa caatcgacct gcgccatcca tctgacattc gctggcatga
    21121 cctaacagta ggcgctgcac acggcgctca accgggttca tgctcgacag acgcgataat
    21181 ttttgctcga taatttccca ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg
    21241 tcaatggtgc aaaccatttg accggcagcg ttctcaagca gctgctcccg ataacgggta
    21301 ggcactgata agcgcccttt gctgtcgaga ttgactaacg ttgctccccg gaacatgcca
    21361 gcctcacccc ttattcccac tttgccccac aatttcccac tgaaaggagt ttacggagcg
    21421 gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc gatgtttacg
    21481 gctaatatca gcgttgttta ttttacgtcc aacgtgcgaa gcaaattaac gttatgaata
    21541 gctgtaagaa aaaatccctc atcgtcatca ccgttttaaa atgactaact attttccaca
    21601 gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt aggacatatc ttccccggtt
    21661 aacagtccct gttgcgtctg tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga
    21721 aaagtgtttg ttaaatcggc aattcatctc tgcatgtaac aaaataatgc aaaaacgcgc
    21781 tatcgatatc acttaattat tgtgaatttc atctggtgag gtaattaacg ggcaggttta
    21841 taagcattta tcaataatag ccaggaatag tcttatttac tttaaggata ttgatgtcca
    21901 ttcccgtact caacgcgcca gagagaaatt cacctgacgc gtatttttgt tcgcggctta
    21961 gctacggctg agcacgccgc ggcgatagag attacgttta atgcgcgtta aaccaggttt
    22021 tggcttacgc ggttcgtcca ggcttgccag gacaatctcc agcacacgct ctgcgacatc
    22081 gcggtgacgt tgagccactg ccagcaccgg gcactgtaag aagtcgagca gttcgttatc
    22141 gccaaaggtg gcaattgcca ggtcagaagg cagtttgccg tcacgacgta gcgtgacatc
    22201 catcacccct tgcaacaacg caaacgacgt tgtgaacagt gcctgcggca tcggatgcgt
    22261 ttccagccat ttttcgaata actgggcagc cgcctcccgc tcatagctgt tggcataaag
    22321 gaaatgcact tcgcgcggat catctttcca ggcagtacgg aaaccttgtt cacgcaggaa
    22381 gctgacagaa agctccggca gcgcgccaag ataaagtacc gtctcggcgg gaaacttacg
    22441 taactcttcc gccagcattt cggcatcatc ctgatcggca ccaaccacgc tggtgaaatg
    22501 ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac gggtcgttag cccagcgttg
    22561 ataaaaagga tgctcaggtg gcaacgacgt cgaaacaata atggcatcaa cctgacgctg
    22621 caaaagatgc tcgatacaac gcatttcgtt gtcgggctga tcttccgagc aggcaatcag
    22681 cagttgataa cccctttgcc gcgcctggcg ttcaagatag ttagcgatgc gggtatagct
    22741 ggtgttctcc agatcgggga tcacaagacc aatagaacgt gtgcgtccag cacgaagccc
    22801 agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc acagccatga ctttttcaac
    22861 ggttttgtcg ctcacacggt attgcttcgc tttgccgtta ataacatagc ttgcagtggt
    22921 ccgcgacact cccgccagcc gagcgatttc atccagtttc acaattgccc cttgcgtaaa
    22981 atgtaaaaac catagccatt gtactggtat gggttaaatt cattaacatc taagcgcaga
    23041 aaattaactg cggcaactgc ttttattcca ggttcccact gatttcgcaa aaaaagccca
    23101 acgtgacatg ttgggctgca aattgcgcat tgagatcatt ctcaacgcat tattttatcg
    23161 ccgcgcgaaa gtccgactac accagagcga gcaacttcca caattttcgc cacatcgcga
    23221 atcgatgcta aaaatgcatc aagcttatcg ctggtgcctg ctaattgaac ggtataaagc
    23281 gagggtgtga catcaataat ttgcccacgg aatatttccg tattacgttt aacttcgtca
    23341 cgcccgtagc cgctggcctg aattttcacc agcatgattt cccgctcaac atgcgcgccc
    23401 tgccctaact cgctcacgcg taatacatcg actagcttgt gtaattgctt ttcgatctgc
    23461 tcaagcactt tttcatcgcc cacggtctgg atggtcatac gcgataatgt cggatcgtca
    23521 gttggtgcaa cggtcaggct ttcaatgttg tagcctcgct gggaaaaaag gccaatcacg
    23581 cgggataacg cgcctgattc attttcgagt aagactgata atatccggcg cataatcagg
    23641 ttctctccgt tttgcttaac cacatttcat ccattccgcc cccgcgaatc tgcatcgggt
    23701 agacgtgctc actgccatcg acggtgacat caacaaacac caggcgatta ttacgcacct
    23761 gttccagcgc ctcgctaagt ttgctttcca gttcttgcgg atgagcaatc tggatcccga
    23821 cgtgaccata ggcttccgcc agacgaacga aatcgggtag agattgcata taagattgtg
    23881 aatgacggcc ggaatagatc atgtcctgcc actgtttcac catccccaga tagcggttat
    23941 tgagattcac taccagtacc ggcaactcgt attgcaacgc ggtagacagt tcctggatgt
    24001 tcatctgaat actgccgtcg cccgtgacgc aaaccacggt ttcttctggc aacgccattt
    24061 tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc gaggccgcca gagttgatcc
    24121 agcgacgcgg tttgtcgaat ggataataaa gcgcagcaaa catctggtgc tgcccgacat
    24181 cggacgtcac gtaagcgtct cccttcgtca accgccaaag agtctcgatc accgcctgcg
    24241 gtttaatctt ttcactgtga gagtcatatt tcaggcactg acgagcgcgc cactgttcaa
    24301 tctgttgcca ccagtcgcgg atctcatcca gcggttgatg gacggattct tgcgacagga
    24361 gttcaagcat ttgttcgagg acctggcgag catcccccac aatcgggata tcagcagtca
    24421 cggttttaga aatggaagta ggatcaatat cgatatgcag aaccgtggca tttgggcagt
    24481 acttcgccag attgttcgtc gttcggtcat caaaacgtac cccgacggca aaaatcacat
    24541 ccgcgttatg catcgtcata ttagcttcgt aggtgccgtg cattcccagc atgcccagtg
    24601 cctgacgatg cgttgccgga aacgccccca gccccattaa tgaggaaaca acgggcaggt
    24661 tcaacgcctc caccgtttct tttagctgct gatgacagcc cgccgtgatt gccccaccgc
    24721 ctacatagac aaccggtttt tttgccgcca ccagcgtttg cagagcacgc ttaatttgcc
    24781 ctttatgccc ggaggtcgtg ggattgtaag atcgcatact gaccgactcc ggccagacgt
    24841 agggtaattt gttcgccgga ttaagaatat ctttgggtaa atcaacgacc acgggcccag
    24901 ggcgaccact tgccgccagc cagaaagcct ttttcagcac ctgcggaatg tcttccgttt
    24961 gcttaaccag aaaactgtgt ttaaccaccg ggcgagaaat ccccaccatg tcgcactcct
    25021 gaaaggcatc gtagcctatc aacgaggtcg ctacctgccc ggaaaggaca actaatggaa
    25081 tggaatccat ataagcggtg gcaatgccag taatcgcatt ggtcgcccct ggacccgacg
    25141 ttaccagcac gacgccgact tccccggtcg cgcgcgccag accatcggcc atatgcaccg
    25201 ccgcctgctc atgacgaact aaaacatgat cgataccacc cacggtatgc aatgcatcgt
    25261 aaatatcaag gaccgcgcct ccgggataac cgaatacttg tttaacgccc tgatcgataa
    25321 gcgatcggac gaccatctcg gctccagaca acatctccat ggcctgcctc actgtttgac
    25381 ggaaaaaaat gtgtaaaaca cattaaccgt ccgatcttgc ttaagcaatt ggcagccaga
    25441 gacataaaca cggggtaata agaataaaca ggaggaaagg tgaaaagaaa cgataaaatc
    25501 ctccattaca gaggattaaa ttgataataa atcggctgaa tcccacaact tacagcaatt
    25561 tctctatttt tttaattaat cctaaaatag agagcacaca cattcagaat aaaattctac
    25621 attcaataaa aaatgcagaa taaaccagac attgatgtct gacctattct gcattcagtt
    25681 agcgtttgca aattgagact aattgctctt ccatccactg atggccttta tcgcgcccgg
    25741 cagcttcatg ccaggagaga taacaggttc tgctgttttg ttttaacggc agcggtaata
    25801 cctgtaattc taaggattca gcgaactctt ccgccagcca acgcggcgca atagcgacca
    25861 gatgcgtttg cgacaccacg ctaagtacgc tcatcattgc catgccctga tacgcgatac
    25921 tggcttgctt atctaccgtg tcataccacg gttgactaaa tgacgcgaaa cgatcgagcg
    25981 aaaccgccgc atgttgttcg ttataaacat catgtttcag taacgggcct ttaattgttg
    26041 gatgattttt gctggctacc agcaccattt catctttaaa taatggcaca ctggtaaatt
    26101 caggacgatg gaagtcttca taactaatca caaactccgt ttcctgataa cgcagctgat
    26161 gttcagtgtt ctgatttaat gaagacttga acataacatg tatatttggc gcaatctgct
    26221 caatgtgatt ataaatctgc gaggtcagaa tgctgtctaa cgggctgcag acacaaagat
    26281 gaaatacacg ttcactactg gcaggctcaa agcctgaacc tggtaattca ttttgtacta
    26341 attgcaatgc ctgacgaact gaaccaaaaa gttgaaatgc gcgagcagtc ggttgaatac
    26401 cacggccata acgaacaaaa agctcgtcat taaacatcac cttcaggcgt gcaacagcgt
    26461 tactgaccgc aggttgcgac attcccagaa catgagcggc acgggtaatg ttttgctcct
    26521 gcatcacggc atcgaaaacg gttaataagt tgagatcgac catgcgtagc tgtggcttgc
    26581 ttaactccgc cgtctctgga tgatctgttt gtacctctgg catacttaac tccactgtca
    26641 cacttaactc cctttccctt attggaatgc agagaaataa tcctcgagaa tatgatttat
    26701 catgcatata aaataagaaa aaggaaaatg agtaaaatta ggaaaacata aagatacggg
    26761 ttcttataaa aacaga
//
LOCUS       DAOTRK010000060        26765 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.060, whole genome shotgun sequence.
ACCESSION   DAOTRK010000060 DAOTRK010000000
VERSION     DAOTRK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.060"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(51..3200)
                     /locus_tag="QIE41_002384"
     CDS             complement(51..3200)
                     /locus_tag="QIE41_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDS3531262.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3223..4416)
                     /gene="acrA"
                     /locus_tag="QIE41_002385"
     CDS             complement(3223..4416)
                     /gene="acrA"
                     /locus_tag="QIE41_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDS3531263.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4558..5205
                     /gene="acrR"
                     /locus_tag="QIE41_002386"
     CDS             4558..5205
                     /gene="acrR"
                     /locus_tag="QIE41_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDS3531264.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5333..8695
                     /gene="mscK"
                     /locus_tag="QIE41_002387"
     CDS             5333..8695
                     /gene="mscK"
                     /locus_tag="QIE41_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDS3531265.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8734..8898)
                     /gene="rsmS"
                     /locus_tag="QIE41_002388"
     CDS             complement(8734..8898)
                     /gene="rsmS"
                     /locus_tag="QIE41_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDS3531266.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(8912..9439)
                     /gene="priC"
                     /locus_tag="QIE41_002389"
     CDS             complement(8912..9439)
                     /gene="priC"
                     /locus_tag="QIE41_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDS3531267.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNMAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9509..9886
                     /locus_tag="QIE41_002390"
     CDS             9509..9886
                     /locus_tag="QIE41_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDS3531268.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10039..10590
                     /gene="apt"
                     /locus_tag="QIE41_002391"
     CDS             10039..10590
                     /gene="apt"
                     /locus_tag="QIE41_002391"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDS3531269.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10719..12650
                     /gene="dnaX"
                     /locus_tag="QIE41_002392"
     CDS             10719..12650
                     /gene="dnaX"
                     /locus_tag="QIE41_002392"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDS3531270.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    11983..12047
                     /gene="dnaX"
                     /locus_tag="QIE41_002392"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12703..13032
                     /locus_tag="QIE41_002393"
     CDS             12703..13032
                     /locus_tag="QIE41_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDS3531271.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13032..13637
                     /gene="recR"
                     /locus_tag="QIE41_002394"
     CDS             13032..13637
                     /gene="recR"
                     /locus_tag="QIE41_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDS3531272.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13747..15621
                     /gene="htpG"
                     /locus_tag="QIE41_002395"
     CDS             13747..15621
                     /gene="htpG"
                     /locus_tag="QIE41_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDS3531273.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15802..16446
                     /gene="adk"
                     /locus_tag="QIE41_002396"
     CDS             15802..16446
                     /gene="adk"
                     /locus_tag="QIE41_002396"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDS3531274.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16578..17540
                     /gene="hemH"
                     /locus_tag="QIE41_002397"
     CDS             16578..17540
                     /gene="hemH"
                     /locus_tag="QIE41_002397"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDS3531275.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17537..18496)
                     /gene="aes"
                     /locus_tag="QIE41_002398"
     CDS             complement(17537..18496)
                     /gene="aes"
                     /locus_tag="QIE41_002398"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDS3531276.1"
                     /translation="MKLENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18648..19952
                     /gene="gsk"
                     /locus_tag="QIE41_002399"
     CDS             18648..19952
                     /gene="gsk"
                     /locus_tag="QIE41_002399"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDS3531277.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20082..21758)
                     /locus_tag="QIE41_002400"
     CDS             complement(20082..21758)
                     /locus_tag="QIE41_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDS3531278.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(21996..23216)
                     /gene="fsr"
                     /locus_tag="QIE41_002401"
     CDS             complement(21996..23216)
                     /gene="fsr"
                     /locus_tag="QIE41_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDS3531279.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKE"
     gene            23434..25086
                     /gene="ushA"
                     /locus_tag="QIE41_002402"
     CDS             23434..25086
                     /gene="ushA"
                     /locus_tag="QIE41_002402"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDS3531280.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLDVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKINGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25123..25602)
                     /gene="ybaK"
                     /locus_tag="QIE41_002403"
     CDS             complement(25123..25602)
                     /gene="ybaK"
                     /locus_tag="QIE41_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDS3531281.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25806..26600)
                     /locus_tag="QIE41_002404"
     CDS             complement(25806..26600)
                     /locus_tag="QIE41_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDS3531282.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISTSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLSQMLR"
BASE COUNT         6605 a         7008 c         7047 g         6105 t
ORIGIN      
        1 gttatgcata aaaaaggccg cttgcgcggc ctttgtgatt acacgttgta tcaatgatga
       61 tcgacagtat ggctgtgctc gatatcttca ttcttgcggc taaagcggcg gcgaaccacc
      121 acaaagaata ccggaacgaa gaagattgcc agtaccgttg cggtcaccat cccgcccatt
      181 acaccggtac ctactgcgtt ctgcgcgccg gaaccagcac cagtactgat aaccagcggc
      241 ataacgccga ggataaacgc cagcgaggtc atcaggatcg gacgtaaacg catccgcacc
      301 gcatcaagcg tcgcttcaat cagaccttta ccttctttat ccatcaagtc tttggcgaat
      361 tcgacgataa gtatcgcgtt cttcgccgac aacccaatgg ttgtgagcag gcctacctgg
      421 aagtaaacgt cattggtcag gccacggaag gtggcagcca gcaacgcacc gataaccccc
      481 agcggaacga ccagcataac ggagaacgga atcgaccagc tctcgtacag cgccgccaga
      541 cacaggaaca cgacaatcaa cgaaatcgcg tacagtgaag gtgcctggtt gccggagaga
      601 cgttcctgat aggacatccc cgtccagtca tagccaacac cggtaggcag tttgctcgcc
      661 agttgttcca tcagctccat tgcttcaccg gtacttttac ccggtgccgc ctggcctaag
      721 atttccatgg atggcaggcc gttgtaacgt tccagacgcg gcgaaccgta ctcccaacga
      781 gaagaggaga acgccgagaa cggcaccatc tgaccatcag cagcacgaac ataccagtcg
      841 ccgatatcat ccggcagcat acggtatttc gcttctgaca tgacgtaaac tttcttcaca
      901 cgaccgcggt cgataaagtc gttcacatag ctaccgcccc atgcagcgcc cagagtggtg
      961 ttaatgtcgt tgatagaaac acccagcgcc tgcgcttttt cctggtcgat atcaatctta
     1021 aactgcgggg tatcttccag accgtttgga cgtacgctgg tcaacatatc agggtgcttc
     1081 gctgcttctg caagcagctg gttacgcgcc tgagtcagtt tttcgtgacc aaggccagcc
     1141 tggtcaatca gctcaaagtc aaagccggtt gcagtaccca gttccacgat tgcgggcagg
     1201 ttaaaggcga aaaccatcgc atctttgatt tgcgagaaag cgcgtgttgc acgcatggta
     1261 atcgcttcaa ctttgttttc ttcacccgga cgatcggccc agtccttcaa ggaaacgaac
     1321 gcaataccgg tattctgacc acgtcccgca aagccgaagc cgttaacggc gaacaccgac
     1381 tcaacgttgt tcttttcttt ggtcagatag taattcgtta cctcattgag cactttctgc
     1441 gtacgttcct gcgttgcacc tgctggcagc tgaaccatgg tcataaatac gccctggtct
     1501 tcatctggca agaaggagct tggcagacgc acgaacagat aggccatgcc gaccacgatg
     1561 atcagataca gcaccaggta acgccctgta ctgcgcagaa taccgcctac gctgtcggtg
     1621 tagtggtgcg tgctcttctc gaacatgcgg ttaaaccagc cgaagaagcc ttttttacct
     1681 tccccgtgat cgcctttggc aatcggtttc agcatggtgg cacaaagagc cggagtcagg
     1741 atcaacgcca ccagtactga cagcgccatt gctgaaacaa tggtaataga gaactggcga
     1801 tagatagcac cagtagaacc gccaaagaag gccatcggaa cgaataccgc cgacagtacc
     1861 atcgcgatac cgaccagagc gccctgaatc tgccccatcg acttacgggt agcttctttt
     1921 ggcggcaaac cttcttccgc catcacacgc tcaacgtttt ctaccacaac gatggcgtca
     1981 tccaccaaca ggccgatggc gagcaccatc ccgaacattg ttagcgtgtt tatcgagaag
     2041 ccaaaggcgg caaggacggc aaaggtcccc agcaatacca ccggtacggc aatggtcgga
     2101 atcaacgtcg cgcggaagtt ctgcaagaac agatacataa ccaggaacac gaggatgatc
     2161 gcttcgacca gcgttttaac cacttcgtga atagagattt tcacgaacgg cgtggtgtcg
     2221 tacgggtaaa caattttcag acctgacggg aagaacggtt ccatcttcgc cagttcagca
     2281 cggattgccg cagcggtatc cagcgcgttt gcaccggtcg ccagcttgat ccccagaccg
     2341 gaagccggtt ggccgttaaa ctctgcgatg atgtcgtagt tctcaccacc cagctcaatt
     2401 ttcgccacat cacgcagcag cacgcgggaa ccatcctgat tcactttcag caggattttg
     2461 ccaaactctt cagtagaggt cagacgcgtc tgagcaataa tagaggcgtt aagctgttgg
     2521 cctttcaccg gcggcgtacc accgagctga cccgccgcaa cctgggcgtt ctgcgctttg
     2581 atggcggtaa tgacatcaac cggtgttagt tggaatttgt tcagttcatt cgggttcatc
     2641 cagatacgca tcgcgtactg tgaaccgaac aactgaacgt cacccacgcc cgacgtacgg
     2701 ctgatggcat ctttcatatt cgccgccacg tagtcggaga tatcctcctg cgtcatggtg
     2761 ccatcggtgt tgataacgcc gacaaccatc aggaagctgc tggatgattt ctcaacgctc
     2821 accccttgct gctgtacttc ttgcggcagc aacggcatcg ccagctgcag tttgttctgc
     2881 acctgaacct gcgcgatatc cgcatcagta ccagactcaa aggtcagggt gatctgcacg
     2941 gtacccgtgg agtcactgtt agaggacatg tacatcaggt tatcgatacc gttcatattc
     3001 tgttcgataa cctgtgtcac cgtgtcctgc actgttttcg catcagcgcc ggggtaggag
     3061 gcggagatcg ttactgccgg cggagcaatc gtaggatatt gcgccaccgg cagtttgagg
     3121 atcgccagcc cccctgccaa catgatgata atggcgatca cccacgcaaa aatcgggcga
     3181 tcgataaaga aattaggcat gtcttaacgg ctcctgttta agttaagact tggactgttc
     3241 aggctgagca ccgcttgcgg cttgctggtt attatcagcg gtaacttctt gtgcttttac
     3301 ctggacacca ggacgcactt tctgcagccc acttattact acgcgatcgc ctgctttcag
     3361 accttctgtc accagccact tatcgccaat agcctggctt gcaacgatcg gacgggtttc
     3421 cactttgtca tccgcgccaa ccaccagtac ggtggcatcg ccacgcggcg tacgggttac
     3481 gccctgttgc gggactaaaa tagcgtttgg attaagccct tcttccagac gtgcgcgcac
     3541 gaacataccc ggcagcagag tgtgatccgg gttcgggaag atagcgcgta gggtgataga
     3601 cccggtggtc tgatcaacgg taacgtcaga gaattccagc gtaccgtcct gcgggaactt
     3661 aatgccgtca ctggtgatca gcgacacttt ggctttgccg ttctcttgtt tcagcgtgcc
     3721 attcgccagt tcctgtttca ggcgcaggaa gtcgttgctg gactgggtca catcaacgta
     3781 gatcggatca agttgctgca cggttgccag cgcagtcgcc tgaccgttct gtaccaatgc
     3841 gccttccgtc acgttcgact taccaatgcg accgctaatc ggagaggtaa ctttggtgta
     3901 agccagattg atccgcgcag tttcaacggc agctttcgcc gcagttaccg cagcattcgc
     3961 ctgttgcgca tcagccagag cctgatcgta ctcttgctta ctgatgtact gagtaccgag
     4021 caatttctga taacgattca ccgtcaattg cgcgatattg gctgcagcct gggctttcgc
     4081 cagatcgcct ttcgcgctgt catatgccgc ctgataggtc gcaggatcaa tctgatagag
     4141 agagacacct gcttcgatgt cgctaccttc tttgaaatta cgcttcagga taatcccgct
     4201 aacttgagga cgaacttctg cgatccggta ggcactggtg cgacccggaa gctcggttgt
     4261 gatctgcaga ggttcagttt tgactgttac tacgccaacg gcgggcatct gctggccacc
     4321 ttgttgggcc tgtttgtcgt cacatcctgt tagggctaag ctgcctgaga gcatcagaac
     4381 gaccgccaga ggcgtaaacc ctctgttttt gttcatatgt aaacctcgag tgtccgattt
     4441 caaattggtc aatggtcaaa agttaataaa cccattgctg cgtttatatt atcgtcgtgc
     4501 tatggtacat acattcacaa atgtatgtaa atctaacgcc tgtaaattca cgaacatatg
     4561 gcacgaaaaa ccaaacaaga agcgcaagaa acgcgccaac acatcctcga tgtggctcta
     4621 cgtcttttct cacagcaggg ggtatcatcc acctcgctgg gtgagattgc aaaagcagct
     4681 ggcgttacgc gcggtgcaat ctactggcat tttaaagaca agtcggattt gttcagtgag
     4741 atctgggaac tgtcagaatc caatattggt gaactagagc ttgagtatca ggcaaaattc
     4801 cctggcgatc cactctcagt attaagagag atattaattc atgttcttga atccacggtg
     4861 acagaagaac ggcgtcgatt attgatggag attatattcc acaaatgcga atttgtcgga
     4921 gaaatggctg ttgtgcaaca ggcacaacgt aatctctgtc tggaaagtta tgaccgtata
     4981 gaacaaacgt taaaacattg tattgaagcg aaaatgttgc ctgcggattt aatgacgcgt
     5041 cgggcagcaa ttattatgcg cggctatatt tccggcctga tggaaaactg gctctttgcc
     5101 ccgcaatctt ttgatcttaa aaaagaagcc cgcgattacg tcgccatctt actggagatg
     5161 tatctcctgt gccccacgct tcgtaatcct gccactaacg aataaccctg aatctgactc
     5221 caggattttt cctggacatt ttcgtcgttg ctattctggt tcactgcgtc gtgatattct
     5281 tgcggtttga ctttttcagg tcgttcttca ggttcagaaa ccttcattca tcatgactat
     5341 gttccagtat tacaaacgat cacggcattt tgttttttca gcatttattg cttttgtttt
     5401 tgtcttgtta tgccagaaca cggcgtttgc gcgggcgtca tcgaatggtg atctgccgac
     5461 aaaagcggac ctgcaggcac aacttgactc actgaataaa caaaaagatc tttctgctca
     5521 ggacaaactg gtgcagcagg atctgacaga tacattagcc accctcgata aaatcgatcg
     5581 cgtaaaagaa gagacagttc agctacggca aaaagtcgct gaagcgccgg aaaaaatgcg
     5641 ccaggcgacc gcggcgttaa ctgcacttag cgatgtcgat aatgacgaag aaacgcgcaa
     5701 aattctgagt acgctttcgt tgcgccagct ggaaactcgc gttgcccagg cgctggacga
     5761 tttgcagaac gcacaaaacg atctggcgtc ttataacagc cagttggttt cgttacagac
     5821 gcagcccgaa cgcgtgcaaa atgcgatgta taacgcttcg cagcagctgc aacaaattcg
     5881 cagtcgtctg gatgggactg atgtcggcga gactgcctta cgtcccagcc agaaagtgtt
     5941 aatgcaggct cagcaggctt tgctgaatgc cgagattgac cagcagcgta aaagcctgga
     6001 agggaacaca gtcttacagg ataccttgca aaagcaacgg gattacgtga cggcgaacag
     6061 cgcccgtctg gagcaccagt tgcagctgtt gcaagaagca gtaaacagca agcgcctgac
     6121 tttaaccgaa aaaacggcgc aggaagccgt ctccccggat gaagccgcgc gtattcaggc
     6181 taatccgctg gtgaagcagg aactggaaat taaccagcag ttaagtcagc gtctgattac
     6241 cgcgactgaa aacggtaatc agttgatgca gcaaaacatt aaagtcaaaa actggctgga
     6301 gcgggcgctg caatcggaac gcaatattaa agagcagatt gccgtcctga agggcagcct
     6361 gctgttgtct cgtatccttt accagcaaca acaaacgctg ccctcggcgg atgagctgga
     6421 aaacatgacc aaccgcatcg cggatttgcg tctcgaacag tttgaagtta accagcagcg
     6481 tgatgcactc ttccagagcg atgcgttcgt caacaaactg gaagaaggtc acaccaacga
     6541 agtcaacagc gaagttcacg atgcgttatt gcaagtagtt gatatgcgtc gcgaattgct
     6601 ggatcagctt aacaaacagt tgggtaacca gctgatgatg gccattaacc tgcaaatcaa
     6661 ccagcagcag ttaatgagtg tgtcgaaaaa cctgaaatcc atcctgactc agcaaatctt
     6721 ttgggtgaac agtaaccgtc caatggactg ggactggatc aaagcgttcc cgcaaagcct
     6781 gaaagatgaa tttaagtcga tgaaaatcac agtgaactgg gaaaaagcat ggcctgccgt
     6841 ttttatcgct ttcctcgccg gtttgccgct gctgttgatt gccgggctga tccactggcg
     6901 tctgggctgg ctgaaagcgt atcaacagaa actggcttcc gctgtgggtt ccctgcgtaa
     6961 cgacagccag ctcaacacac caaaagcgat ccttatcgac ctgatccgtg cgctgccggt
     7021 gtgcctgatt attctcgcgg ttggcctgat tctgttgacc atgcaactca acatcagcga
     7081 actgctatgg tcgttcagta aaaaactggc gattttctgg ctggtgtttg gcctgtgctg
     7141 gaaggtactg gagaaaaacg gcgttgccgt gcgtcacttt ggtatgccgg aacagcagac
     7201 cagccactgg cgtcggcaaa ttgtccgcat cagtctcgca ttgctgccta tccatttctg
     7261 gtctgtggtg gcagaacttt ccccgctgca tctgatggat gatgtgctgg ggcaggcgat
     7321 gattttcttc aacctgctgc tgattgcctt cctggtatgg ccgatgtgcc gcgaaagctg
     7381 gcgtgataaa gagtcgcaca ccatgcgact ggtcaccatt accgtgctgt cgattatccc
     7441 gattgcgttg atggtgctga ccgccacggg ttatttctac accacgctgc gcctctccgg
     7501 gcgttggatt gaaaccgttt atttggtgat catctggaac ctgctgtacc agacggtatt
     7561 gcgcggctta agcgtagcgg cgcggcgtat cgcctggcgt cgtgcgctgg cgcgtcggca
     7621 gaatctggtg aaagagggcg cggaaggtgc tgaaccgccg gaagaaccca ccattgcact
     7681 ggagcaggtt aaccagcaga cgctgcgtat taccatgttg ctgatgtttg cgctgttcgg
     7741 tgtcatgttc tgggcaattt ggtccgattt gatcaccgtg ttcagctatc tggacagcat
     7801 cacgctctgg cattacaacg gcactgaagc tggcgctgcg gtggtgaaaa acgtcaccat
     7861 gggcagtctg ttgtttgcga ttatcgcctc aatggtagcc tgggcgttga ttcgcaacct
     7921 gcctggtctg ttggaagtgt tggtgctctc gcgcctgaac atgcggcagg gcgcgtcgta
     7981 tgccattact accatcctta actacatcat tattgccgtt ggtgcgatga cggtgttcgg
     8041 atcgctgggc gtctcgtggg ataaactcca gtggctggcg gcagcattat ccgtaggtct
     8101 tggttttggt ttacaagaga tcttcggtaa cttcgtctcc ggtttgatca ttctgttcga
     8161 acgtccggtg cgcattggcg atacggtaac cattggtagc ttctcgggga cggtaagtaa
     8221 gatccgtatt cgtgcgacaa cgattaccga tttcgatcgc aaagaagtga tcatcccgaa
     8281 caaagcgttt gttaccgagc gtctgatcaa ctggtcgttg actgacacta ctacgcgtct
     8341 ggtgatccgt ctcggcgtgg cctatggctc cgatctggaa aaagtgcgta aagtgttact
     8401 gaaggcggcg actgagcacc caagggtgat gcacgaacca atgccggaag tcttctttac
     8461 ggcgtttggt gccagcacgt tggatcatga gctgcgtctg tatgtgcgtg aactgcgtga
     8521 ccgtagccgt actgtcgatg agctgaaccg tactatcgat cagctgtgcc gtgaaaacga
     8581 tatcaacatt gcctttaacc agcttgaagt gcatctgcac aacgagaagg gcgatgaggt
     8641 gacggaagtc aaacgcgact acaaaggcga cgatccgaca ccagcggtag ggtaaggaca
     8701 ataaagggcg acagtgatgc cgccctttat tttttactcg ctttgcgaag cctcatcgct
     8761 ctgtaacttc ttttcataat cacgcttcac aatatccagc gcgcgcagca cggtgcgggc
     8821 tgggatctga ttttcttcca gcagcacaat caaatcgaca gccagtttga catcgtcagg
     8881 ggcattttcc agtgacatac tctctccatt gctagcgggt taagcgcgct agcctgtttt
     8941 cgattttttc cagcgcatgg cggcagcgtg ccaggcgcgc ttcataggct tccacttcac
     9001 gatgcagcgt ttgctgttcc acgagatcgg tcacccgcgc caggcgggct cgacgttcgg
     9061 ctaccatctc acgtagccgc cgctcaaaat cctgatgctg aatacgttta cgctgccagc
     9121 gggcaatttt cggtggcgca ctgtcccact cgcgtagcga ccaggctgtg gcttcacgcg
     9181 ctatagcctc cagttgcgcc gccagatgtt ctgccagcca ggccacttgc ggcagctgtt
     9241 gctgctcaac cgcatgacga agcgcagcca tgttatcgcc cgcctcatcg agacaagcct
     9301 gcagtgttgt cgcacgggtc tgaaaaaggt gcctgtcaaa gcgggcactt agcgtggcaa
     9361 attgcgctac cggggcacaa cgctggcgca gcgtggcgag ctgtccttcc agtttttcca
     9421 gcagcagggc ggttttcatg ataagacctc aatgaaaatg ataattgtta tgctaaagta
     9481 gccactctgt aagctgacaa cactcaatat gcaacgaatc attttaatca tcattggctg
     9541 gctggcggta gtgctgggta cgctgggcgt ggtattaccg gtattaccga cgacgccgtt
     9601 tatcctgctg gcggcctggt gctttgcccg ttcttccccg cgctttcacg cctggttgct
     9661 gtaccgctca tggtttggca gctatctacg tttctggcag aaacatcatg cgatgccgcg
     9721 cggcgtcaaa ccgcgggcga ttttgcttat tttgctcacg tttgcaattt ctctgtggtt
     9781 cgtccagatg ccatgggtgc gcatcatgtt gctggtaatt ctcgcctgtt tgctttttta
     9841 tatgtggcga attccggtga ttgatgaaaa gcaagaaaag cactgaagca caacaatcgc
     9901 agttgcaatt attgcgtaca gccagtacat tctggcgttt tcgagcacag gcgcaggcgg
     9961 tcaaaggtta aacaactgtt acttttgata cgtttaaaac gcgccgtgag taccaccgta
    10021 acaagcaggc atacacttat gaccgcgact gcacagcagc ttgagtatct caaaaatagc
    10081 atcaaaagca ttcaggacta cccaaaaccc ggcattcttt tccgtgatgt caccagctta
    10141 ctggaagacc cgaaagctta cgctctcagc atcgacttgc tggttgagcg ttacaaaaat
    10201 gcgggcatta ccaaagttgt cggcaccgaa gcgcgtggct tcttgtttgg cgctccggta
    10261 gctctgggtc tgggcgttgg ctttgtaccg gtccgtaaac cgggcaaact gccgcgtgaa
    10321 accatcagtg aaacttacga tctggaatac ggcaccgatc agctggaaat ccacgttgat
    10381 gccatcaaac cgggcgacaa agttctggtg gtggacgatc tgctggcaac cggcggcact
    10441 atcgaagcga ccgttaaact gatccgtcgt ctgggtggtg aagtggctga tgctgcgttc
    10501 attatcaatc tgttcgatct cggcggcgaa cagcgtctcg aaaaacaggg cattaccagc
    10561 tacagccttg ttccgttccc gggccattaa ttatcgccag tcttgtgctg cccacgctac
    10621 ggacagcaca agatgtgcat tcagcctcgc cgttctgacg gggctgtgtt agcattaccc
    10681 cttcgtgaat ccaccttcca gcgtttcaga gcctgccaat gagttatcag gtcttagccc
    10741 gaaaatggcg cccacaaacc tttgctgacg tcgtcggcca ggaacatgtg ctgaccgcac
    10801 tggcgaacgg cttgtcgtta gggcgtattc atcatgctta tcttttttcc ggcacccgtg
    10861 gcgtcggaaa aacctctatc gcccgactgc tggcgaaggg gctaaactgc gaaaccggca
    10921 ttaccgcgac gccgtgcggc gtgtgcgata actgtcgtga aatcgagcag gggcgctttg
    10981 tcgatctgat tgaaatcgat gccgcctcgc gcactaaagt tgaagacact cgcgacctgc
    11041 tggataacgt ccagtacgct ccggcgcgtg gtcgtttcaa agtttatctg atcgacgaag
    11101 tgcatatgct gtcgcgccac agctttaacg cactgttaaa aacccttgaa gagccgccag
    11161 agcacgttaa gtttctgctg gcgacgaccg atccgcagaa attgccggtg acgattttgt
    11221 cacgttgtct gcaatttcat ctcaaggcgc tggatgtcga gcaaattcgc catcagcttg
    11281 agcacatcct caacgaagaa catatcgctc acgagccgcg ggcgctgcaa ttgctggcac
    11341 gcgccgctga aggcagcctg cgagatgcct taagtctgac cgaccaggcg attgccagcg
    11401 gtgacggcca ggtttcaact caggcggtca gtgcgatgct gggtacgctt gacgacgatc
    11461 aggcgctgtc gctggttgaa gcgatggtcg aggccaacgg cgagcgcgta atggcactga
    11521 ttaatgaagc cgctgcccgt ggtatcgagt gggaagcgtt gctggtggaa atgctcggcc
    11581 tgttgcatcg tattgcgatg gtacaacttt cgcctgctgc acttggcaac gacatggccg
    11641 ctatcgagct gcggatgcgt gaactggcgc gcaccatacc gccgacggat attcagcttt
    11701 actatcagac gctgttgatt ggtcgcaaag aattaccgta tgcgccagac cgccgtatgg
    11761 gcgttgagat gacgctgttg cgcgcgctgg cgttccatcc gcgtatgccg ctgcctgagc
    11821 cagaagtgcc acgccagtcc tttgcgcccg tcgcgccaac ggcagtaatg acgccaaccc
    11881 aggtgccgcc gcaaccgcaa tcagcgccgc agcaggcacc gactgtaccg ctcccggaaa
    11941 ccaccagcca ggtgctggcg gcgcgccagc agttgcagcg cgtgcaggga gcaaccaaag
    12001 caaaaaagag tgaaccggca gccgctaccc gcgcgcggcc ggtgaataac gctgcgctgg
    12061 aaagactggc ttcggtcacc gatcgcgttc aggcgcgtcc ggtgccatcg gcgctggaaa
    12121 aagcgccagc caaaaaagaa gcgtatcgct ggaaggccac cactccagtg atgcagcaaa
    12181 aagaagtggt cgctacgccg aaggcgctga aaaaagcgct ggaacatgaa aaaacgccgg
    12241 aactggcggc gaagctggcg gcagaagcca ttgagcgcga cccgtgggcg gctcaggtga
    12301 gtcaactttc gctaccaaaa ctggtcgaac aggtggcgtt aaatgcctgg aaagaggaga
    12361 gcgacaacgc agtatgtctg catttgcgct cctctcagcg gcatttgaac aaccgcggcg
    12421 cacagcaaaa actggctgaa gcgttgagca tgttaaaagg ttcaacggtt gaactgacta
    12481 tcgttgaaga tgataatccc gcggtgcgta cgccgctgga atggcgtcag gcgatatacg
    12541 aagaaaaact tgcgcaggcg cgcgagtcca ttattgcgga taataatatt cagaccctgc
    12601 gtcggttctt cgatgcggag ctggatgaag aaagtatccg ccccatttga tcgtaagcac
    12661 agcttacgtt cgtcatcctt aacgtgattg agagagaaac ctatgtttgg taaaggcggt
    12721 ctgggtaacc tgatgaagca agcccagcag atgcaagaaa aaatgcagaa aatgcaggaa
    12781 gagatcgcgc agctggaagt caccggcgaa tctggcgcag gtctggtaaa agtgaccatt
    12841 aacggtgcac acaactgccg tcgcgtagag atcgacccga gcctgctgga agacgacaaa
    12901 gagatgctgg aagacttggt ggctgcagca ttcaacgacg cagcacgtcg tattgaagaa
    12961 acgcagaaag aaaaaatggc ctctgtatcc tccggaatgc agctgccgcc tggctttaag
    13021 atgccgttct gatgcaaacc agcccgctgt taacacagct tatggaagca ctgcgctgtc
    13081 tgccgggcgt tggcccgaag tcggcgcagc gtatggcgtt cacgctgctt cagcgcgatc
    13141 gtagcggcgg gatgcgtctg gcgcaggcgc tcacccgggc gatgtcggaa atcggccact
    13201 gcgccgattg ccgcaccttc accgaacagg aagtctgtaa catctgttcg aatccgcgtc
    13261 gtcaggaaaa cggtcaaatc tgcgtggtgg agagtccggc ggacatctac gccattgagc
    13321 agacggggca gttttcaggc cgttattttg tgttgatggg gcacctgtca ccgctggacg
    13381 gcatcggtcc ggatgatatc ggccttgatc gtctggaaca gcgtctggca gaggaaaaaa
    13441 tcactgaagt gatcctcgcc accaacccca ctgttgaagg tgaagctacc gctaactaca
    13501 ttgccgagct ttgcgcgcaa tatgacgtgg aagccagccg aatcgctcat ggcgtaccgg
    13561 ttggcggcga actggaaatg gtcgatggca ccacgctgtc acactccctt gccgggcgtc
    13621 ataagattcg tttttaagca aacgagagca ggatcacctg ctctcgcttg aaattattct
    13681 cccttgtccc catctctccc acatcctgtt tttaacctta aaatggcatt attgaggtag
    13741 acctacatga aaggacaaga aactcgtggt tttcagtcag aagtgaaaca gcttctgcac
    13801 ctgatgatcc attctctcta ttccaataaa gaaatcttcc tgcgtgagct tatctctaac
    13861 gcctccgatg cggcggacaa gctgcgtttc cgcgcgctct ctaacccgga cctgtacgaa
    13921 ggtgatggcg aactgcgcgt tcgtgtctct ttcgataaag acaagcgtac gctgaccatc
    13981 tccgataacg gcgtagggat gacccgcgac gaagtgattg accatctggg gactatcgct
    14041 aaatccggca ccaaatcatt cctcgaatcc ctgggttctg accaggcgaa agacagccag
    14101 ctgatcggtc agtttggtgt tggtttctac tctgcgttta tcgtggccga caaagtgacc
    14161 gtgcgtactc gcgcggcagg cgaaaaacca gaaaacggtg ttttctggga atcggctggt
    14221 gaaggtgaat acaccgttgc cgacatcacc aaagaagatc gtggtactga aatcaccctg
    14281 catctgcgtg aaggcgaaga cgagttcctc gatgactggc gcgtgcgttc catcatcagc
    14341 aaatactccg accatatcgc gctgccggta gagatcgaaa aacgcgaaga gaaagacggc
    14401 gaaaccgtta tctcttggga aaaaatcaac aaagcgcagg cgctgtggac tcgtaacaag
    14461 tcggaaatca ccgatgaaga gtacaaagag ttctacaaac acatcgccca cgactttaac
    14521 gatccgctga cttggagcca caaccgcgtt gaaggtaagc aggagtacac cagcctgctg
    14581 tacattccgt ctcaggcacc gtgggatatg tggaaccgcg atcataaaca cggtctgaaa
    14641 ctgtatgttc agcgtgtgtt catcatggac gacgcagaac agttcatgcc gaactatctg
    14701 cgcttcgtgc gtggtctgat tgactccagc gatctgccgc tgaacgtttc ccgtgaaatc
    14761 ctccaggaca gcacggtaac gcgtaacctg cgcaatgcgc tgaccaagcg tgtgctgcaa
    14821 atgctggaaa aactggcgaa agacgacgcg gaaaaatacc agaccttctg gcaacagttt
    14881 ggcctggtac tgaaagaagg tccggcggaa gatttcgcta accaggaagc gatcgccaaa
    14941 ctgctgcgtt ttgcttctac ccataccgat tcttctgcgc agaccgtgtc tctggaagac
    15001 tacgtttccc gcatgaaaga agggcaggag aaaatctact acattaccgc agacagctat
    15061 gcggcagcga agagcagccc gcacctggaa ctgctgcgta agaaaggcat cgaagttctg
    15121 ctgctttccg accgcatcga tgagtggatg atgaactatc tgactgagtt cgacggtaaa
    15181 ccgttccagt cggtgtctaa agttgacgag tcgctggaaa aactggctga cgaagttgat
    15241 gagagcgcga aagaagcgga gaaagcactg actccgttca tcgaccgtgt gaaagccctg
    15301 ctcggcgagc gtgtgaaaga tgtccgtctg actcaccgtc tgaccgatac gccagcgatt
    15361 gtctctaccg atgcggacga aatgagcacc cagatggcga aactgttcgc tgcggcgggc
    15421 cagaaagtgc cagaagtgaa atacatcttt gaactgaacc cggatcacgt actggtgaaa
    15481 cgtgcggcag atactgaaga tgaagcgaag ttcagcgagt gggtagaact gctgctggat
    15541 caggcgctgc tggcagaacg cggcacgctg gaagatccga acctgtttat tcgtcgtatg
    15601 aaccagctgc tggtttcctg atgtcatgcc ggatgacctt cgtgtcatcc ggcatttttc
    15661 ttttcattat ctgcactttc cgcaaattat ctcgccatta accgtttcag ccccaggtgc
    15721 ctttcttgag gcaatcgcct gttggtggta tcgtttatcg ctttttcaaa aaattcgaca
    15781 cattttaagg ggattttcgc aatgcgtatc attctgcttg gcgctccggg cgcggggaaa
    15841 gggactcagg ctcagttcat catggagaaa tatggtattc cgcaaatctc cactggcgat
    15901 atgctgcgtg ctgcggtcaa atctggctcc gagctgggta aacaagcaaa agacattatg
    15961 gatgctggca aactggttac cgacgaactg gtgatcgcgc tggttaaaga gcgcattgct
    16021 caggaagact gccgtaatgg tttcctgttg gacggcttcc cgcgtaccat tccgcaggca
    16081 gacgcgatga aagaagcggg catcaatgtt gattacgttc tggaattcga cgtaccggac
    16141 gaactgatcg ttgaccgtat tgtcggtcgc cgcgttcacg cgccgtctgg tcgtgtttat
    16201 cacgttaaat tcaacccgcc gaaagtagaa ggcaaagacg acgttaccgg tgaagaactg
    16261 actacccgta aagacgatca ggaagaaacc gtacgtaaac gtctggttga ataccatcag
    16321 atgactgcac cgctgatcgg ctactactcc aaagaagcgg aagcgggtaa caccaaatac
    16381 gcgaaagttg acggcaccaa gccggttgct gaagttcgcg ctgatctgga aaaaatcctc
    16441 ggctaatcaa gccgcagcag atctgctttc gggcaggtct gctcattctc ctcaggcaat
    16501 acctctcaca gcaattagtt cttcttcttc acttttccgc tacaattatc aacaagttga
    16561 atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctagca aacctgggta
    16621 cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt ttaagcgaca
    16681 gacgcgtggt tgatacctca cggttgttat ggtggccatt gctgcgcggc gtgattttgc
    16741 cactgcgctc gccgcgtgtg gcgaagctgt atgcctctgt ctggatggag ggtggctcgc
    16801 cgctgatggt ttacagccgc cagcaacagc aggcgctggc acaacgttta ccggagacgc
    16861 ccgtagcgct gggaatgagc tacggctcgc catcactgga aagcgccgta gatgaactcc
    16921 tggcagagca tgtagatcat attgtggtac tgccgcttta tccgcaatac tcctgttcaa
    16981 cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt agcattccgg
    17041 ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca ctggcgaaca
    17101 gcgtacgtgc ttctttcgct aaacatggcg aaccggatct gctgctgctc tcttatcatg
    17161 gcattcccca gcgttatgca gatgaaggcg atgattatcc gcaacgttgc cgcacaacga
    17221 ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg acctttcagt
    17281 cgcgctttgg gcgggaaccc tggctgatgc cttataccga cgaaacgctg aaaatgctcg
    17341 gagaaaaagg cgtaggtcat attcaggtga tgtgcccggg ctttgctgcg gattgtctgg
    17401 agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcg ggcgggaaaa
    17461 aatatgaata tattccggcg cttaatgcca ctccggaaca tatcgagatg atggctaatc
    17521 ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg cgaagagcct
    17581 cgtcggcggt tttcatcatc cgtgaataat gcaaaaaggc gtgcagcgtg cctgggtaga
    17641 gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat
    17701 ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg cgagtgagat
    17761 cattattaaa caggcagtaa tacggcgact cgcggtccgc gtcgttgctt aaatacgcct
    17821 cttcatacat ctgtaaatcc tgttgcgtta agccatccca gacaccgccc aacagacgac
    17881 gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacgccc gcaactttac
    17941 cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc atggcacctg
    18001 cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc gcctgctggt
    18061 ggaaataaca acaagcagcc acaatttcct ctatcgcttg cggaaaacgc gcttcaggtg
    18121 aaagggtgta atcaatacca atcaccgtac attggctgta gcttgccagc aggcgcatga
    18181 tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa taaaatagcg
    18241 tcgctgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca tattttgttg
    18301 gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat cggcgctcaa
    18361 gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccagggc ggtaaatccg
    18421 gctgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga acgggtaatt
    18481 tgttttccag cttcatgcaa aaactccttt caaattacgt cattgtaagg aaccactgcc
    18541 atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc gctcccgtca
    18601 gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg aaatttcccg
    18661 gtaaacgtaa atccaaacat tacttccctg taaatgcacg cgatccgctg cttcagcaat
    18721 tccagccaga aaacgaaacc agcgccgcct gggtagtggg tatcgatcaa acgctggtcg
    18781 atattgaagc gaaagtggat gacgaattca ttgagcgtta tggattaagc gccgggcatt
    18841 cactggtgat tgaggatgac gtagccgaag cgctttatca ggaactaaaa cagaaaaacc
    18901 tgattaccca tcaatttgcg ggtggcacta ttggtaacac catgcacaac tactcggtgc
    18961 tcgcggacga ccgttcggtg ctgctgggcg tcatgtgtag caatattgaa attggcagct
    19021 atgcctatcg ttacctgtgt aacacctcca gccgtaccga tcttaactat ctacaaggcg
    19081 tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa cgtacctttg
    19141 ctatcagccc tggccacatg aaccagctgc gggctgaaag tattccggaa gatgtgattg
    19201 ccggagcctc ggcactggtt ctcacttctt atctggtgcg ttgcaagccg ggtgaaccca
    19261 tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta ccggtggtgc
    19321 tgacgctggg caccaagttt gtcattgccg agaatccgca gtggtggcag caattcctca
    19381 aagaccacgt ttctatcctt gcgatgaacg aagatgaagc cgaagcgttg accggagaaa
    19441 gcgatccgtt gttggcatct gacaaggcgc tggactgggt tgatctggtg ctgtgcaccg
    19501 ccgggccaat cggcttgtat atggcgggct ttactgaaga cgaagcgaaa cgtaaaaccc
    19561 agcatccgct gctgccgggc gctatagcgg aatttaacca gtatgagttt agccgcgcca
    19621 tgcgccacaa ggattgccag aatccgttgc gtgtctattc gcacattgcg ccgtatatgg
    19681 gcgggccgga aaaaatcatg aacactaatg gagcggggga tggcgcactg gcagcgttgc
    19741 tgcatgacat taccgccaac agctaccacc gtagcaacgt accaaactcc agcaaacata
    19801 aattcacctg gttgacgtat tcgtcattag cgcaggtgtg taaatatgct aaccgtgtga
    19861 gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg gagcgtgaag
    19921 acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag gccagataag
    19981 gcgttcacgc cgcatctggc atttggctct cgatgcctga tgcgacgctg gcgcgtctta
    20041 tcaggcctac atatccgcgc ctattatccg gcaaccaccg tttaccccgt caccacctca
    20101 cccgccggtg gcgtttcaag cagttccagc atagtacggg cgatttcacg ctcacccatc
    20161 actacctgat ttgcaccacg ttcggtgata tacgccactt catcgtcata atgggcgcgg
    20221 gcaataatct caatatccgg atttttcgcg cgggcagatg ccacaatctc acccgcttca
    20281 taaccgttgg gaatcgtcag gatcagccat tttgcacatt ccagatgcgc cagttgcata
    20341 atttcttcgt tcgccgcatt gcccaatact gcgcggaccc cgcgctctcg cagttcatca
    20401 acacgggttc gtgacgtctc aatcaccact aaaggaatat cagaggcgag caatttctcc
    20461 cccagcaggc tgcctacacg accgtaacct accagtagcg catggttgca aatatccact
    20521 gggatctgct tctcttcttc gatagcctct tccagcgtct gctcttccag cgtttcggtc
    20581 ttcgccagat atttctccag cagcgcaaac aggaccgggt tgagcataat cgacaaaatc
    20641 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    20701 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat agttaacgcc
    20761 gtgcgctggg agtgaccaaa cagacgcaca aggaacagtg ccgcgaccga tttaccaaac
    20821 agaataatcg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    20881 aacaacatcc cgacggagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcagcgta
    20941 tcgtgggcgg cacggtgact cagttcagac tcgttcagca ccatcccggc aaagaacgca
    21001 ccgagtgcaa aggagacatc aaacagctcc accgcaccaa aggcaatccc taacgccagc
    21061 gccagcaccg acagggtaaa cagctcacgg gaaccggttg ctgcgctgcg tgccataatc
    21121 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    21181 atggtgatcc ccatatcgac agcaagagtg gcaaagccca catcgccttg ttccatcatt
    21241 cctgccactg cgggcagcaa caccagcgtc agaaccatta ccaggtcttc cacaatcaac
    21301 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    21361 agtaacacca cggtactggc ggtggaaagg cataagccga acacgatacc ggtcattaac
    21421 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    21481 atcgcaccgg gaatggcgat ggcctttacc gccatcaaat ccttcagcga aaagtgcaaa
    21541 ccgacgccaa acatcaacag aatgacgccc agttcagcca gttccggcgc aagcttggta
    21601 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    21661 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    21721 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    21781 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    21841 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    21901 gcacggatcg gaaaactgaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaat
    21961 gatgcagcca aagtttgcct ttggctgcaa tgaaatcact ctttatgccg gttatcaggc
    22021 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    22081 atgctggtgt ggtcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa
    22141 gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagcag ctcctgagcg
    22201 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    22261 cccgtccagt gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    22321 gagccccaaa tcacatattt tcgcccaatt ttatccccta caggcccgcc gatcaccgta
    22381 ccggccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    22441 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    22501 ttcgagaaaa tgaggattaa cagaatgctg accgccagta ccactttgtt gcgcggcagt
    22561 ggattgataa tcgtcgcttt tggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    22621 cggctgattt gcgccaacac cacgatcgcc agcagtgccg caagtacaaa ccaggctacg
    22681 ttgcctttgc cataaggcgc gataattacc gctgccagca agggccccag ggaactgcca
    22741 aagttgccgc cgacctgaaa gatagattgc gccaggccat gacgcccgcc agaagccata
    22801 cgggccacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    22861 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    22921 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtaaccgacc
    22981 accggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    23041 aatgtcagag aaaactctga ctgaagcagc ggataaatcg ccagaatcag ggattggatc
    23101 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    23161 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    23221 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    23281 aacatacctg tatgcgtcgc ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    23341 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    23401 tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    23461 ttagcactgt taaccacatt tacactggcg agtgaagctg ctctggcgta tgagcaggat
    23521 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    23581 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    23641 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    23701 cccgagtctg acttacagga tgccgaacct gattttcgcg gcatgaatct ggtgggctac
    23761 gatgcgatgg caatcggtaa tcatgaattt gataacccgc tcaccgtatt acgccagcag
    23821 gaaaagtggg caaagttccc gttactttct gccaatatct accagaaaag taccggcgag
    23881 cgcctgttta aaccatgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggc
    23941 ctgacgacgg atgacacagc gaagttaggc aacccagaaa acttcaccga tattgagttc
    24001 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    24061 gacattatta tcgcggcgac ccatatgggg cattacgaca atggtgagca cggctctaac
    24121 gcaccgggcg atgtggagat ggcgcgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    24181 ggtggtcact cgcaagctcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    24241 gtgccgggta cgccatgcaa accggatcaa caaaacggca tctggattgt gcaggcgcat
    24301 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    24361 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    24421 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    24481 tcaccgttcc agaacaaagg caaagcgcag ctggacgtga aaataggcga aaccaatggt
    24541 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttaatt
    24601 ctggcagccc aaatggatcg cactggtgct gactttgcgg tgatgagcgg aggcggaatt
    24661 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    24721 ggcaatgtgg tggtgtatgc cgacatgacc gggaaagagg tgattgatta cctgaccgcc
    24781 gtcgcacaaa tgaagccaga ttcaggtgcc tacccgcaat ttgctaacgt tagctttgtg
    24841 gcgaaagacg gcaaactcaa cgacctgaaa atcaacggcg aaccggtcga tccggcgaaa
    24901 acttaccgca tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt
    24961 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    25021 atccagaaga gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    25081 cagtaatccg aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat
    25141 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    25201 cccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    25261 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccagat atcccgtcga
    25321 acgctgcgca accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    25381 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    25441 accgttcact gcgaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    25501 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    25561 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    25621 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    25681 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    25741 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    25801 tttttttagc gcaacatctg cgacaaattc ccttctccat acaggtgtag tgcaccgact
    25861 gcgaccacat agcgccccgg cggcatggcg cataatttgt cccgccaggc gagatttcgc
    25921 tgatgcatca gcacatcgta cagcgattga ctgaacgtat taggcagcgt tatttcatta
    25981 ttttgcggcg gcgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcattggtg
    26041 tgccagtggg tcagcgtatc gtccagcagt gccagtccgt tgtcagggag ctggagcaac
    26101 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    26161 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    26221 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgaggtagaa
    26281 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    26341 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    26401 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    26461 atatcgtggc tgcccatatg aatactgcca atcagatgaa aatggcgatt gccgggaaga
    26521 gagatatcga tagcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    26581 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcgggga
    26641 gggatggcgc aaatggagtt atgaggcttg ttcagtggta gcttaaatat gatcgaaaaa
    26701 ttgtttttgc tttgaaacga aagactgcac cggtgttggt ggcgtcaaaa tcgccgaggc
    26761 gttcc
//
LOCUS       DAOTRK010000061        26572 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.061, whole genome shotgun sequence.
ACCESSION   DAOTRK010000061 DAOTRK010000000
VERSION     DAOTRK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.061"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            251..1021
                     /locus_tag="QIE41_002405"
     CDS             251..1021
                     /locus_tag="QIE41_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDS3531283.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTVAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1012..1485
                     /locus_tag="QIE41_002406"
     CDS             1012..1485
                     /locus_tag="QIE41_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDS3531284.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1592..2131
                     /gene="dnaT"
                     /locus_tag="QIE41_002407"
     CDS             1592..2131
                     /gene="dnaT"
                     /locus_tag="QIE41_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDS3531285.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2134..2871
                     /gene="dnaC"
                     /locus_tag="QIE41_002408"
     CDS             2134..2871
                     /gene="dnaC"
                     /locus_tag="QIE41_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDS3531286.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2920..3414
                     /locus_tag="QIE41_002409"
     CDS             2920..3414
                     /locus_tag="QIE41_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDS3531287.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3668..5959
                     /gene="opgB"
                     /locus_tag="QIE41_002410"
     CDS             3668..5959
                     /gene="opgB"
                     /locus_tag="QIE41_002410"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDS3531288.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLFGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6099..7121)
                     /gene="lgoD"
                     /locus_tag="QIE41_002411"
     CDS             complement(6099..7121)
                     /gene="lgoD"
                     /locus_tag="QIE41_002411"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDS3531289.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIANLKNGQQVAVIPYVACQQCPTCKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7260..8174
                     /locus_tag="QIE41_002412"
     CDS             7260..8174
                     /locus_tag="QIE41_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDS3531290.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8389..9750
                     /locus_tag="QIE41_002413"
     CDS             8389..9750
                     /locus_tag="QIE41_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3531291.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9799..11463)
                     /gene="tsr"
                     /locus_tag="QIE41_002414"
     CDS             complement(9799..11463)
                     /gene="tsr"
                     /locus_tag="QIE41_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDS3531292.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11582..12028)
                     /gene="hpaR"
                     /locus_tag="QIE41_002415"
     CDS             complement(11582..12028)
                     /gene="hpaR"
                     /locus_tag="QIE41_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDS3531293.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12300..13589
                     /locus_tag="QIE41_002416"
     CDS             12300..13589
                     /locus_tag="QIE41_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDS3531294.1"
                     /translation="MKGTIFAVALNHRSQLDVWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTSDLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13586..15052
                     /gene="hpaE"
                     /locus_tag="QIE41_002417"
     CDS             13586..15052
                     /gene="hpaE"
                     /locus_tag="QIE41_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDS3531295.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15054..15905
                     /gene="hpaD"
                     /locus_tag="QIE41_002418"
     CDS             15054..15905
                     /gene="hpaD"
                     /locus_tag="QIE41_002418"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDS3531296.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15915..16295
                     /locus_tag="QIE41_002419"
     CDS             15915..16295
                     /locus_tag="QIE41_002419"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDS3531297.1"
                     /translation="MPHFIVECTENIRQEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16363..17166
                     /gene="hpaH"
                     /locus_tag="QIE41_002420"
     CDS             16363..17166
                     /gene="hpaH"
                     /locus_tag="QIE41_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDS3531298.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17177..17965
                     /gene="hpaI"
                     /locus_tag="QIE41_002421"
     CDS             17177..17965
                     /gene="hpaI"
                     /locus_tag="QIE41_002421"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDS3531299.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            18083..19459
                     /gene="hpaX"
                     /locus_tag="QIE41_002422"
     CDS             18083..19459
                     /gene="hpaX"
                     /locus_tag="QIE41_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDS3531300.1"
                     /translation="MSDTSPAIPESIDPANQHKTLTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            19469..20359
                     /gene="hpaA"
                     /locus_tag="QIE41_002423"
     CDS             19469..20359
                     /gene="hpaA"
                     /locus_tag="QIE41_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDS3531301.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            20610..22172
                     /gene="hpaB"
                     /locus_tag="QIE41_002424"
     CDS             20610..22172
                     /gene="hpaB"
                     /locus_tag="QIE41_002424"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDS3531302.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            22190..22702
                     /locus_tag="QIE41_002425"
     CDS             22190..22702
                     /locus_tag="QIE41_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDS3531303.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23095..25245
                     /gene="btsT"
                     /locus_tag="QIE41_002426"
     CDS             23095..25245
                     /gene="btsT"
                     /locus_tag="QIE41_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDS3531304.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25295..25498
                     /locus_tag="QIE41_002427"
     CDS             25295..25498
                     /locus_tag="QIE41_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDS3531305.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            25509..26465
                     /gene="yjiA"
                     /locus_tag="QIE41_002428"
     CDS             25509..26465
                     /gene="yjiA"
                     /locus_tag="QIE41_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDS3531306.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
BASE COUNT         6302 a         7135 c         6893 g         6242 t
ORIGIN      
        1 aacgcgccca gttatctttt atcctgattc gtaaaggcta aacaataaat tcattatagt
       61 gataatattc atgtgataat ttatttgcga cgagagataa ttcgcttttt atcaccgagc
      121 ttctcccgat aaccaggatt aatcagcgca attttgatct taacctaccg caaaacaacc
      181 gtgcttcatg atacaaatgg attctcatta tctgaacaat acgcagcacg tctatgacaa
      241 agggcgagtt atgcaaactg agcaacagcg agccgtaaca cggctttgta tccagtgtgg
      301 attatttctt ttgcaacatg gtgcggaaag cgcgttggtt gatgagcttt cctcacgact
      361 gggtcgggcg ttgggaatgg acagcgtcga aagttctatc tcttcgaacg ccatagtgct
      421 gacaaccatt aaagatgggc aatgcctgac atcgacacga aaaaatcacg atcgcggcat
      481 taatatgcat gtggtgactg aagtccagca cattgtgatt ctggcggagc atcatctgct
      541 ggattacaaa ggtgtagaga aacgatttag ccaaattcag ccattacgtt acccgagatg
      601 gctggtagcc ttaatggtcg gcctttcttg cgcctgtttc tgtaaactca ataaaggtgg
      661 ctgggatggt gccgtcatca ccttctttgc cagtacggtc gcgatgtata tccgccagct
      721 gctggcacaa cgtcatcttc atccacagat caacttttgc ctcaccgctt tcgccgccac
      781 caccatttcc ggtttgcttt tgcaacttcc cactttcagc aatacaccca ccatcgcgat
      841 ggccgccagc gttctgctgc tggtgccggg ttttccgttg attaatgccg tcgccgatat
      901 gtttaaaggc cacatcaata ccggactggc acgctgggcg atcgccagtc tgctgacact
      961 ggctacctgc gtcggcgtgg tgatggcact gacgatttgg gggctacgcg gatgggtgtg
     1021 atcgaatttc tgttagcgtt ggcgcaggat atgatcctcg ccgccattcc tgcggtcggc
     1081 tttgcgatgg tgttcaacgt tcccgtgcgg gcgttacgct ggtgtgcgct gcttggatcg
     1141 ataggtcatg gttcccgaat gatcttaatg accagcgggc tgaatattga gtggtcaact
     1201 tttatggctt ctatgctggt cggcaccatt ggtattcaat ggtcgcgctg gtatctggcg
     1261 catccgaaag tgtttaccgt ggcggctgtt attcctatgt tccctggcat atcggcttat
     1321 accgcaatga tttcggcggt aaaaatcagc cagctaggtt acagcgaacc gttgatgatt
     1381 accctgttaa ccaactttct tacagcttca tcaattgttg gtgcgttatc catcggtctt
     1441 tccattcctg gattatggtt gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa
     1501 gcacacggat gagagacagc ctcctctcct ccgtgtgtta ctataaaagt tatctccctt
     1561 ctcgttcatc gttccatatt tgagaaacag tatgtcttcc agagttttga ccccggacgt
     1621 cgttggtatt gacgccctgg tacacgatca ccaaaccgtt ctggcaaaag ctgaaggcgg
     1681 tgtggttgcc gtatttgcta acaatgcccc ggcgttttat gccgtcacgc ctgcacgcct
     1741 ggctgaactg ctagcgctgg aagaaaagct ggcgcgtccg ggaagcgatg tcgctctgga
     1801 cgatcaactc tatcaggaac cgcaagctgc tcccgttgct gtacccatgg ggaaattcgc
     1861 catgtatccg gactggcaac ccgatgccga ctttatccgc ctggcggcgc tatggggcgt
     1921 ggcactaaga gagccggtga ccaccgaaga actggcctca ttcattgcct actggcaggc
     1981 ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa ctggcgcgca gcctgcaaat
     2041 cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg aatacggtca gtgaacctga
     2101 cagccaaatt ccaccaggat tcagagggta acgatgaaaa acgttggcga cctgatgcaa
     2161 cgcctgcaaa aaatgatgcc tgcccatatc aaacctgctt tcaaaacggg tgaagaacta
     2221 ctggcctggc agaaagaaca aggggcgatc cgctccgccg ctctcgaacg tgaaaatcgg
     2281 gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc gtccactgca tcagaactgc
     2341 tcctttgaga actatcgcgt tgagtgtgaa gggcagatga atgcgttaag caaagcgcgc
     2401 cagtatgtcg aagagtttga cggcaacatc gccagcttca tcttttctgg taagccagga
     2461 accggcaaaa accatctggc ggcggcaatc tgcaacgagc tgctgctacg cggtaaatcc
     2521 gtattgatca tcaccgtggc cgatattatg tcggcgatga aagatacctt caggaatagc
     2581 ggtaccagcg aagaacaact acttaacgat ctgagcaacg ttgatctgct ggtgatcgat
     2641 gagatcggcg tgcagaccga atcgaaatac gaaaaagtga tcatcaacca gatcgtcgat
     2701 cgccgctctt cttccaaacg cccaaccggg atgctgacca acagtaatat ggaagagatg
     2761 accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc tgggtaacag tttgtgggtg
     2821 atcttcaact gggatagcta ccgtagccgg gtaacgggta aagagtatta atccccgttt
     2881 ctggcggtat actgcgacac aattccacct tgagtaatga tgatgaaaac tgttaaacat
     2941 cttctgtgct gtgccattgc cgccagcgca ttaatttcca ccggggtgca tgctgcgtcc
     3001 tggaaagatg cgctctccag cgccgccagc gaacttggca accaaaacag cacgacacag
     3061 gaaggcggtt ggtcgctcgc gtcattaact aacttgctta gcagcgggaa ccaggcctta
     3121 agcgcagata acatgaacaa tgccgcaggc attctgcaat actgcgcgaa gcaaaagctg
     3181 gcttcggtaa ccgatgccga aaacatcaag aatcaggtac tggaaaaact gggcctgaac
     3241 agtgaagagc aaaaagaaga caccaactat ctggatggca ttcagggttt gctgaaaacc
     3301 aaagatggtc agcaactcaa tctggataat attggaacga ctccgctggc agaaaaggtg
     3361 aaaaccaaag cctgcgatct ggtgttaaag caggggctga acttcatttc ctgaaccgcg
     3421 aaacgccgcg ttttagctgt tacccggctg acgcggctct ctcctgccct tttccctgct
     3481 cctggacggt tttccccctg ttacacggga agcgaccgcg atcaaaaaag tctgtttata
     3541 aactaattcc taacaacatc gcattttttt gacactagaa ttgcagcaat atggcgacgc
     3601 cattacattg tgcttctcaa aaagcttaaa cgttggctaa acggcccggt ttagtgagga
     3661 tcgcccgttg tcagaactac tctctttcgc cctttttctc gcctctgtgc tgatttacgc
     3721 atggaaagcg ggacgtaaca cctggtggtt tgcagccaca ttaacggtac tggggctatt
     3781 tgtcgtttta aatatcaccc tgtttgccag cgactatttt actggcgatg gtattaacga
     3841 cgcggttctc tataccttaa ccaacagcct gaccggtgct ggtgtcagta aatacatcct
     3901 gcctggtatc ggcattgtgc tggggctgac ggcggtgttc ggtgcgctgg gctggatcct
     3961 gcgccgtcgt cgccatcatc cgcaccattt tggttacagc ctgctggcac tcttactggc
     4021 gctgggctca gtggatgcca gcccggcatt tcgtcagata acggaactag tgaaatccca
     4081 gtcacgcgac ggcgacccgg actttgcggc ttattataaa gagccgtcga aaactatccc
     4141 tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt ctcgagcgga cctattttga
     4201 taacgaggct ttcccggatc tcacgcctga actgggcgcg ttgaaaaatg aaggcctgga
     4261 tttcagccac acgcagcagc tgccgggaac agattacacg attgctggca tggtggcttc
     4321 tcagtgcggc ataccgctgt ttgccccctt tgaaggtaac gcctccgcct ctgtctccag
     4381 cttcttcccg cagaacatct gtctgggcga tatcctgaaa aactcgggtt atcagaacta
     4441 tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat gtgttcctga aatcgcacgg
     4501 cttcgaccac ttattcggct cagaagagct gaaaagcgtg gtggccgacc cgcactatcg
     4561 caacgactgg ggattctacg acgataccgt tctcgatgaa gcgtggaaaa agtttgaaga
     4621 gctttcccgc tcaggtcagc gattctcact gtttaccctg acagtcgata cccatcaccc
     4681 ggatggtttt atctcgcgta cctgtaaccg caaaaaatat gattttgacg gtaagccgaa
     4741 tcagtcattc agcgcggtaa gttgcagcca ggagaatatc gcgacgttta tcaacaaaat
     4801 caaagcgtca ccgtggttta aagataccgt catcgtcgtc tcttctgacc atttagcgat
     4861 gaacaacacg gcgtggaaat accttaataa acaggatcgc aataacctgt tttttgtcat
     4921 ccgtggcgac aagccgcagc aagagacgct ggcggtgaag cgtaacacga tggataacgg
     4981 cgcgacggtg ctggacattc tcggtggcga taactatctc ggacttggtc gtagcagttt
     5041 atccgggcag tcaatgtcgg aaatcttcct caatatcaaa gagaaaacat tggcgtggaa
     5101 gccggatatc atccgcctgt ggaaattccc taaagagatg aaagagttca ccatcgacca
     5161 gcagaaaaac atgattgcct tctcgggtag ccatttccgt ttgccgctgc tgttgcgggt
     5221 ttcagacaaa cgcgtggaac cgctgccgga aagtgaatac tcagcaccgc tgcgtttcca
     5281 gctggccgat ttcgctccac gcgacaattt cgtctgggtt gaccgttgct acaagatggc
     5341 acaactttgg gctccggaac tggcactctc caccgactgg tgtgtctcgc aagggcagct
     5401 tggcggtcag caaattgtcc agcatgttga caaaacaaca tggaagggca aaacggcatt
     5461 taaagatacg gtcatcgaca tggcgcgtta caaaggcaat gtcgatacgc tgaagattgt
     5521 tgataacgat attcgctaca aagccgacag tttcatcttt aacgtcgccg gtgcgccaga
     5581 agaggtgaaa cagtttagcg ggatttcccg tccggagtcg tggggccgct ggtccaacgc
     5641 gcaactgggc gatgaagtaa aaatcgagta caagcatccg ctgccgaaga aatttgacct
     5701 ggtgattact gccaaagcat acggcaataa cgccagccgt cctattccgg tacgcgtagg
     5761 caatgaagaa caaactcttg tgctgggcaa tgaagtgacc accaccacac tgcatttcga
     5821 taacccaacc gatgccgaca cactggtaat tgtgccgccg gaacctgtct caaccaacga
     5881 agggaatatc ctcggacact cgccgcgtaa gctcgggatc ggcatggtgg aaattaaagt
     5941 tgtggaacgt gaagggtaat ttctgactga tgtgtaggtc gcataaggtg cgacctacac
     6001 accttctccg gcctacaaaa catcattgag aaacagaaaa aaaacagcgc ccaacacttc
     6061 cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat tacaccctta
     6121 atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacggg
     6181 tagcgatggg ttaacatcat gtcggcagtg atttttcctt ccgccatcag tcgaccgact
     6241 ttagcgaaat cttccggcgt ggcgttgcgg ctacccatca tcgtcgtttc ttttttatgg
     6301 aattccggat cggagaactg caactcacct ttaaacagac cgacaaatac caccgtgccg
     6361 ccgtgacgaa tcagattcac ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc
     6421 actttctgcg ccagcgaacc accaaactgc gcccgcagct gcgcgtcaaa atcttcggct
     6481 gacgggtcca gtacaggtaa ttccagacgc gttgccacat gttcacggcg cgccggactg
     6541 gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg
     6601 attggccccg ccccgaccac cagcacctgc tcgccgggag caatggctgc gcgacgcacc
     6661 gcatgagcgc taatagcgaa aggttcaatt aatgctgccg cctgcgggtc aataccatct
     6721 gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc
     6781 acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgtcgggcat
     6841 tgctgacagg caacataagg gatcaccgct acttgctgtc catttttaag atttgcaata
     6901 tttttaccca gcccaacaat ctccccacat atttcatggc ctaaaacgcg tggataacta
     6961 aaaaatggtt gattcccacc ccaggcatga atatcggtac cacaaatgcc gactgactta
     7021 atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat
     7081 tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct ctccattttt
     7141 tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg
     7201 aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata
     7261 tgagtcgttc acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc
     7321 ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca
     7381 gccgcactac agtgcgtcat attctcagcc acttacgcga atgcggcgtc ttaacgcagg
     7441 ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga tgacggtttc gcctgtacta
     7501 ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg atgataaacc
     7561 agcgccagtt acgccctggg gaaacttttt ctgaactgca actggctcgg gccgcgggtg
     7621 tcagcccggt tgtagtaaga gaatatcttt taaaattcgg ccgttataat cttattcaaa
     7681 gcgaaaagcg cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt
     7741 ttgaactacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc
     7801 atgatccccg ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca
     7861 acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc
     7921 tttcagctgc cgacaatatc ttttttgatc aatctcttga gatcatctca gtcatctttc
     7981 attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg
     8041 agcatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc acccttgcgc
     8101 tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat gagaacacgc
     8161 gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca cggttttatc
     8221 aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg actaaaaatg
     8281 tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatatc aaccttgcgt
     8341 aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt ggaaaaagaa
     8401 aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga tattcccgtg
     8461 ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac caccgccatg
     8521 ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct gtcggtagca
     8581 aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc tttgctctcc
     8641 gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg gcccactatt ggatcgtaaa
     8701 ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca ggccatgtct
     8761 ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa
     8821 gcgccgatga acccatgtgg cgtaaaagtc attaacgact ggttcaacat caaagagcgc
     8881 ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgccgt aagcccaccg
     8941 attctggcgg cgatgatgct ggtgatgggc tggcgtggga tgtttattac cattggtgtg
     9001 ctggggattt ttctcgccat tggctggtat atgctctatc gcaaccgcga gcacgtagaa
     9061 ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg ccgagatccg
     9121 ctcagttttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg aatgatgctc
     9181 ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc tggttacctg
     9241 caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat ccctttcctg
     9301 tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt caaaggggga
     9361 atggctccga ttaaaagccg taagatctgc attattgccg ggatgttctg ttctgccgcc
     9421 tttacgctgg tcgtaccgca agcgacaaca tccatgacgg cggttctgct gattggcatg
     9481 gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca cgtcgcagtt
     9541 gcttctcgca tgacggcgtc ggtgggcagt atccagaact ttgccagctt catctgcgcc
     9601 tcttttgcgc cgatcattac tggttttatt gttgatacca cccactcatt ccgtctggca
     9661 ctaatcatct gcggttgcgt caccgcagcg ggagcactgg cgtacatctt cctggttcgt
     9721 cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca ttttgcttat
     9781 cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg ccacggccat
     9841 tttacgcggc gtagctggcg tcacggtttt tactacagcc gatgtttcac gctgctgttg
     9901 ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg cctgctcttc
     9961 cagcgcggcg gcggcagcgg cagattcttc caccagcgca gcgttctgtt gagttacccg
    10021 gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc
    10081 agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcgctgacaa tctccgccat
    10141 tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc
    10201 aatcaggctt ttaatttcac gagccgcctg ggcgctgcgc tgggccagat tacgcacttc
    10261 tcccgcgacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgcggc
    10321 gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga taatatcggc
    10381 gattttctgc gaactggtgg agatatcgcg catggtctgt actacgttat ccaccacttt
    10441 gccgccgcgt tgcgccgttt cagaagcact taacgccaga tggctggcct gacgcgcatt
    10501 ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg tctcttccag
    10561 cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct
    10621 ggcaccgcta taaatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc
    10681 tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc
    10741 aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat
    10801 tggcgctacc aacgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac
    10861 gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattat tatcgctgac
    10921 ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc
    10981 gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaacag
    11041 ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc
    11101 tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc agtttttttc
    11161 cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc
    11221 aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg
    11281 caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac
    11341 agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg
    11401 taaaaggcca aaaacggcca aaaccagcag taagctggtc acaattttga tacgttttaa
    11461 catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta
    11521 tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag atattaaaaa
    11581 gttattcatt atcgcccggg atgtcttctt gtcgggaatt acccagagca ataaattcct
    11641 ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat
    11701 aggcttcttc aatctgtgtc tgagcgcggt tatacagggc ctgcccctct ttggtcagcg
    11761 agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt
    11821 ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat
    11881 cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag
    11941 tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga
    12001 gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca aaatcattaa
    12061 tatagaaaca gttaatcatc acgttaccgg gactgagcta tccccgcaat tcctcatttt
    12121 atcaaaattg agcatacttt attaacttac tgatatttag tattttatta cctgcaaatg
    12181 taaatagttt gttaattaga tcacatttac atcacttact tttgccaaac tacgcaatcg
    12241 ggaataaaag taatcattaa catattaatg attaaaatga tcgaatccga ggagtggtaa
    12301 tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccagcttgat gtatggcagg
    12361 aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg tttattaaac
    12421 cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt gaaaaggtac
    12481 tgagcggtgc gacagttgcg ctgattgtgg gaaaaacggc gacgaaagta cgtgaagaag
    12541 atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg ccggaagaga
    12601 gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa
    12661 ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac gggcgtcctg
    12721 ccgatcactg gaacacctcc gatttacaac gtaacgccgc gcagttgctg agcgccctga
    12781 gcgaatttgc cacgctgaat ccaggcgatg ccattctgct cggcacgcca caggcgcgcg
    12841 tggaaataca gccaggcgat cgcgtgcgtg ttctcgcaga aggtttcccg ccgctggaaa
    12901 atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg cagccacacc
    12961 cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc gaactggaat
    13021 ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc gaataccctc acgggcgata
    13081 accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg gagctggtgg
    13141 tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg
    13201 gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac taccgcccta
    13261 acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc gtgccgaaag
    13321 aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc
    13381 agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc tacttaagcg
    13441 aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa ggcttatctg
    13501 acgtggtgcc tggtgatgaa gtagtggtgg aagtagaagg cgtgggccgc ctggtgaacc
    13561 gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa
    13621 tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag tgctggcaga
    13681 tgtggcctcc ggcggtgaag cggagatcaa tcaggcggta gcggcagcga aagaggcgtt
    13741 cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga
    13801 tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca cgggcctgcc
    13861 gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg aatttttcgc
    13921 ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga tgctcaacta
    13981 cacgctggtg caaccggtag gcgtttgtgc gctggtatcg ccgtggaacg tgccgtttat
    14041 gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg tgctgaagat
    14101 gtcggaactc tccccgctga ccgctgaccg cctgggtgag ctggcgctgg aagccggtat
    14161 tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg atgcgctggt
    14221 tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggcgaccg ggcgcaacat
    14281 catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat cgccggtgct
    14341 gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca ccatcttctc
    14401 gatcaacggc gagcgctgca ccgccggttc gcgcatcttt attcagcaaa gcatctaccc
    14461 ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga
    14521 tccgaatacc caggttgggg cgcttatcag ccagcagcac tgggaaaaag tctctggcta
    14581 tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc
    14641 tgacctgcct gcacatctga aaggcggcaa cttcctgcgc ccaacggtgc tggcagacgt
    14701 agataaccgt atgcgcgtcg cccaggaaga gattttcggg ccggtcgcct gcctgctgcc
    14761 gtttaaagac gaagccgaag gtctgcgcct ggcaaacgac gtggagtacg gcctcgcgtc
    14821 atacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca ttgaagcagg
    14881 catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg gcggcgtaaa
    14941 agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa
    15001 gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct gatatgggta
    15061 agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct gaactgccag
    15121 ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc agcaagcgtt
    15181 gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg gtcaacagtg
    15241 cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac gagctgccgc
    15301 attttattcg tgacatgacc tacaactacg agggcaaccc ggagttgggg cagcttattg
    15361 ccgatgaagc cttaaaactc ggcgtgcggg caaaagcgca caatattccc agcctgaaac
    15421 tggaatacgg cacgctggtg ccaatgcgct acatgaatga agacaagcac ttcaaagtgg
    15481 tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag cttggcgaag
    15541 cgattttgaa agcaatcgaa cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt
    15601 tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc tacacccgcg
    15661 agttcgaccg ccagatggac gagcgcgtgg tgaagctgtg gcgcgaaggc cagttcaaag
    15721 agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc aatatgcacg
    15781 acacggtgat gctgctgggg atgctcggct gggataaata tgacggcaag gtggagttta
    15841 ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc ccgcttcccg
    15901 cgtaaggaac caccatgccg cactttatcg tggaatgtac tgaaaatatt cgtcaagaag
    15961 cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcgacg gggatttttc
    16021 cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg gctgatggca
    16081 agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcgggcgc agtctggaaa
    16141 gccgtcagga agtaggcgaa atgctgtttg atctgattaa aacgcacttc gcctcactga
    16201 tggagagccg ttacctggcg ctttcatttg agattgcaga gctgcacccg acgttgaatt
    16261 tcaaacagaa caacgtacac gcgttgttta aataaatttg taccagcttc caggccggat
    16321 gataaggcgc agccgcatcc ggcaatgcca caggatatcg ccatgttcga taaacacacc
    16381 cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca gatccgcgcg
    16441 atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtaca gcgcgaatgg
    16501 gtccgcctga aaattgccga aggccgtacg ctgaaaggcc acaaaatcgg cctgacctcg
    16561 aaagcgatgc aggccagctc gcaaatcagc gaaccggatt acggcgcgct gctggacgac
    16621 atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc gcgcattgaa
    16681 gtggagctgg ctttcgtgct ggcaaaaccg ctgcgtgggc caaactgcac gctgttcgac
    16741 gtctacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc tcgctgccac
    16801 aacatcgatc cggaaaccca gcgcccgcgt aaagtgttcg acaccatttc tgataacgcc
    16861 gccaatgccg gggtgatcct cggtggtcgt cccattaagc ccgatgagtt ggatctacgt
    16921 tggatctccg ccctgatgta tcgcaacggc gtgattgaag aaaccggcgt cgccgctggc
    16981 gtgctgaatc acccggcaaa cggcgtggcc tggctggcga acaaactcgc accgtatgac
    17041 gtgcaactgg aagccgggca aatcattctc ggcggttcgt tcacccgccc ggttccggcg
    17101 cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gttccattag ctgccgcttt
    17161 gtttaaggag agaacgatgg agaacagttt taaagcggcg ctgaaagcag gccgcccgca
    17221 gatcggatta tggctggggc tgagcagcag ctacagcgca gagttactgg ccggagcagg
    17281 attcgactgg ttattgatcg acggtgagca cgcgccgaac aacgtgcaaa ccgtgctcac
    17341 ccagctacag gcgattgcgc cctaccccag ccagccggta gtacgtccgt cgtggaacga
    17401 tccggtgcaa atcaaacaac tgctggacgt cggcacacaa accttactgg tgccgatggt
    17461 tcaaaacgct gacgaagccc gtgaagcggt acgcgccacc cgttatcccc ccgccggtat
    17521 tcgcggcgta ggaagcgcgc tggcacgcgc ctcgcgctgg aaccgtattc cggattacct
    17581 gcaaaaagcc aacgatcaaa tgtgcgtact ggtgcagatc gaaacgcgtg aggcaatgaa
    17641 gaacttaccg cagattctgg acgtggaagg cgtcgacggc gtgtttatcg gcccggcgga
    17701 tctgagcgcc gatatgggtt atgccggtaa tccgcaacac ccggaagtgc aggccgcgat
    17761 tgagcaggcg atcgtgcaga tccgcgaagc gggtaaggcg ccgggtatcc tgatcgccaa
    17821 tgagcaactg gcaaaacgct acctggaact gggcgcgcta tttgtcgccg tcggcgttga
    17881 caccaccctg ctcgcccgcg ccgccgaagc gctggcagca cggtttggcg cgcaggccac
    17941 gaccgttaag cccggcgtgt attaatgctg aatgcgctta tccggcttac agcagtacca
    18001 aactgtagcc ggataaggcg tttacgccgc atccgccata aatcttgtcg taccctacaa
    18061 aaatcccatt agaggaagaa aaatgagcga cacctcacct gccataccgg aaagtatcga
    18121 tccggcaaat cagcataaaa cgctgactgc cggacaacag gcggttatta agaagctatt
    18181 tcgccgcctg atcgtctttc tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa
    18241 catcggcttt gccgggctaa cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg
    18301 cctcgccacc accctgttct atgccgctta tgtcatcttc ggcattccca gcaacattat
    18361 gctgagtatt gtcggtgcgc ggcgctggat cgccaccatc atggtgctct ggggcatcgc
    18421 ctctacggcc accatgtttg ccactggccc caccagctta tacgtactgc gtatactggt
    18481 tggcattacc gaagccggct ttctgcctgg cattctgctg tatttaacct tctggtttcc
    18541 ggcctatttc cgcgcccgtg ccaacgcctt gtttatggta gcaatgccgg taacgacagc
    18601 gctgggatcg atcgtttccg gctacatttt gtcgctggat ggcgtaatgg cattaaaagg
    18661 ctggcagtgg ctgtttttgc tggaaggctt cccgtcggta ttactcggcg tcatggtgtg
    18721 gttctggctt gatgactcac cggacaaagc taagtggctg acgaaagaag acaaaaaatg
    18781 cctgcaagag atgatggata acgatcgtct gacgctggtt cagccagagg gagccatcag
    18841 tcaccacgcc atgcaacaac gcagcatgtg gcgggagatc ttcactccgg tggtgatgat
    18901 gtataccctg gcgtatttct gcctgaccaa cacacttagt gcaatcagca tctggacacc
    18961 gcagatccta caaagcttta atcagggcag cagtaatatc accatcggcc tgctggccgc
    19021 cgtaccgcag atttgtacca ttctcgggat gatctactgg agccgtcact cagatcgccg
    19081 ccaggaacga aggcatcaca ccgcccttcc ttatttgttc gctgccgctg gttggttact
    19141 ggcttcggca actgatcaca acatgatcca gatgctgggg atcattatgg cttcgaccgg
    19201 atcattcagc gcaatggcga ttttctggac aacaccggat cagtccatca gcctgcgggc
    19261 acgagcgatc ggtattgcgg tgatcaacgc cactggcaac attggttcag cgttaagtcc
    19321 gtttatgatc ggctggttga aagatctgac cggcagcttt aacagtggat tgtggtttgt
    19381 tgccgcgctg ctggtgattg gtgcggggat tatctgggca attccaatgc agtcctcccg
    19441 tccgcgagcg accccgtaag gaacgacgat gtgtgaccgt cagattgcca atattgatat
    19501 cagcaaagag tacgatgaaa gcctgggcac ggacgatgtg cattatcagt ccttcgcccg
    19561 catggcggca ttttttggcc gccatatgct gccacatcgc cacgaacagt actttcagat
    19621 gcatttcctc aatagcggac agattgagct acagcttgac gatcatcgct actcggtgga
    19681 agcgcccctg tttgtcctga cgccgccgtc agtacctcat gcgtttatta cggagtctga
    19741 cgccgacggt catgtgttga cggtacggga agatctgatc tggcccctgc tggaagttct
    19801 ttatccgggc actcgggaaa ccttcggcct gccggggatt tgcctgtcac tggcagataa
    19861 acccgacgaa ctggcagcgc tggaacacta ttggcaactg atagagcggg aatcggtgga
    19921 acaactgcct ggacgggaac acaccctgac gttactggca caggcagtat tcaccctact
    19981 gctgcgtaac gcaaaactcg acgaccatgc cgccagcggg atgcgcggag aattaaaact
    20041 gttccagcgt tttaatatgc tgattgaaag ccattttcat cagcactgga cagtaccgga
    20101 ttacgctaac gaactgcata tcaccgaatc acgtctcacg gacatctgcc gccgctttgc
    20161 caaccgtccg ccaaaacggt tgattttcga caggcagctg cgagaagcca agcggctgct
    20221 gctgttttct gataacgccg tgaacaatat tgcctggcaa ctcggtttta aggatccggc
    20281 ttattttgcg cgctttttta atcgtttagt cggttgctcg cccagtgctt atcgtgccaa
    20341 aaaagtacct gtgacgtgaa aatcccttag ccttaacggg aaaccaggca ccacctgcta
    20401 ttcccctttc tccgcctgga gcaaggggat ttagtaacgt caaaaatcgc taaaagcgat
    20461 ctcgatcaca aaaccgtacc cgacctgaaa agtaccagcc attgtcccaa aagtgtcttc
    20521 ccgtaatcgc ccctgcaagc ttcaattgaa taacaaaaac aacacacaaa attaacaatc
    20581 gtattccgat taatactgta gaggtcgaca tgaaaccaga agatttccgc gccagtaccc
    20641 aacgtccgtt caccggggaa gagtatctga aaagcctgca ggatggtcgc gagatctata
    20701 tctacggcga gcgagtgaaa gacgtcacca ctcatccggc atttcgtaat gcggcagcgt
    20761 ctgttgccca actgtacgac gcgctgcaca aaccggagat gcaggactct ctgtgttgga
    20821 acaccgacac cggcagcggc ggctataccc ataaattctt ccgcgtggcg aaaagtgccg
    20881 acgacctgcg ccagcaacgc gacgccatcg ctgaatggtc acgcctgagc tatggctgga
    20941 tgggccgtac cccagactac aaagccgctt tcggttgcgc actgggcgcg aatccgggct
    21001 tttacggtca gttcgagcag aacgcccgta actggtatac ccgtattcag gaaactggcc
    21061 tctactttaa ccacgcgatt gttaacccac cgattgatcg tcatttgccg accgataaag
    21121 tgaaagacgt ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg
    21181 cgaaagtggt tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg
    21241 cacaagtgat gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg
    21301 atggcgtgaa attaatctcc cgcgcctctt atgaaatggt ggcgggtgct accggctcgc
    21361 cgtacgacta cccgctctcc agccgctttg atgagaacga tgcgattctg gtgatggata
    21421 acgtgctgat cccatgggaa aacgtactga tctaccgcga tttcgatcgc tgccgtcgct
    21481 ggacaatgga aggtggtttt gcccgtatgt atccgctgca agcctgtgtg cgcctggcag
    21541 tgaaattaga cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg
    21601 agttccgtgg tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg
    21661 cattgagtga ctcgatgtgt tctgaagcga cgccgtgggt caacggggct tatttaccgg
    21721 atcatgccgc actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa
    21781 acattatcga acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc
    21841 tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg
    21901 atcacgtcca gcgcatcaag atcctcaaac tgatgtggga tgccattggc agcgagtttg
    21961 gtggtcgtca cgaactgtat gaaatcaact actccggtag ccaggatgag attcgcctgc
    22021 aatgtctgcg ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc
    22081 gctgcctgtc ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg
    22141 atatcaacat gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga
    22201 tgaacaacgc ctgcgctttc gtgacgcgat ggccagcctg tcggcagcgg taaatattat
    22261 caccaccgag ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac
    22321 ggatacacca ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt
    22381 tcagggcaac ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg
    22441 ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca
    22501 aaaaggtccg ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggtgagat
    22561 ccgcgatgtg caggcaattg gcacacatct ggtgtatctg gtggagatta aaaacatcat
    22621 cctcagtgca gaaggtcacg gacttatcta ctttaaacgc cgtttccatc cggtgatgct
    22681 ggaaatggaa gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt
    22741 tattgtgatc tcaatcacat atccacccga tgaataattc ttgcccaacc gctcaccgcc
    22801 attcctgacc gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgcg
    22861 cgtaactctg gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca
    22921 aacctagaac ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga
    22981 aggcagcaag tgagtgaatc cccgggagct tacaacagta agtgacaggg gtgaacgaac
    23041 gcagctgccg cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat
    23101 acgaaaaaac tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc
    23161 ctcgcggtag ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc
    23221 tctgtgtcgg tgtatctggt ggcttatcgc tactacagtc tgtacatcgc ccagaaggtg
    23281 atgaaactcg accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac
    23341 gttccgacca accgttacgt gttgttcggt caccacttcg ccgctatcgc cggtgctggt
    23401 ccgctggtgg gtccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg
    23461 ctggcggggg tagtgctggc cggtgcggta caggacttta tggtgctgtt tatttcctct
    23521 cgccgtaacg gcgcatctct cggtgagatg atcaaagaag agatgggacc agtaccgggg
    23581 actatcgcgc tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg
    23641 attgtggtta aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta
    23701 ccgattgcgc tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa
    23761 gtctctgtca ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct
    23821 cacgatccgt actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg
    23881 attggctatg cgtttgtttc cgcactactg ccggtgtggc tgatcctcgc accgcgcgac
    23941 tatctggcaa ccttcctgaa aatcggtgtt atcgtcggcc tggcgctggg tatcgtggtg
    24001 ctgaacccgg aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg
    24061 tggaaaggcg ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc
    24121 ttccacgcac tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg
    24181 cgtttcattg gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt
    24241 gctgcgtcca tcatcgaacc gggtctttac ttcgcgatga ataccccgcc tgctggcctt
    24301 ggcatcacca tgcctaacct gcatgaaatg ggtggcgaaa acgcgccgat catcatggcg
    24361 cagctgaaag acgttaccgc acatgcggca gcgaccgtca gctcctgggg cttcgtgatt
    24421 tcgccagagc agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt
    24481 gcaggtggtg cgccgacgct ggcggtaggt atcgctcatg tgttccacaa agtgctgccg
    24541 atggctgaca tgggcttctg gtatcacttc ggtattctgt tcgaagcact gttcatcctg
    24601 accgcgctgg atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac
    24661 ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc
    24721 tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt
    24781 aagagcctgt ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta
    24841 ctgggcaccg ttgtgctaat taagatgaag cgcacccaat acatctgggt aactgttgtt
    24901 ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg gcctgaaact gttcagcacc
    24961 aacccgcaga tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac
    25021 ggtactgacc tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac
    25081 accaacgcag gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt
    25141 ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac
    25201 gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg
    25261 cttcggcggg gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa
    25321 aaaatatctc ggccaagcgg cgaagatgtt gattggcatt ccagactacg acaactacgt
    25381 cgagcatatg aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg
    25441 cgaacgccag aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg
    25501 gagagagcat gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa
    25561 ccaccctgct gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa
    25621 acgaattcgg cgaagtctcc gttgatgatc aattgattgg cgaccgcgcc acgcagatca
    25681 aaacgctgac caacggctgc atctgctgtt cgcgctccaa cgagctggaa gacgcgctac
    25741 tcgacctgct ggataatctc gacaagggca atattcagtt cgatcgtctg gtcattgaat
    25801 gcaccggcat ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat
    25861 gccagcgcta cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc
    25921 agatgaacca gttcaccatc gcccagtccc aggtgggcta cgctgaccgt attctgctga
    25981 ctaaaaccga cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg
    26041 cccgcgcacc ggtctacacc gtcacccacg gtgacatcga cctgggcctg ttgttcaaca
    26101 ccaacggttt tatgctggaa gaaaacgtcg tcagcaccaa accgcgtttc cactttatcg
    26161 cggataaaca aaacgatatt tcgtcgattg tggtggaact ggattacccg gtagatatca
    26221 gcgaagtttc ccgcgtgatg gaaaacctgc tgctggaatc ggcagataaa ctgctgcgtt
    26281 acaaagggat gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc
    26341 gcctctacag cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacgatgg
    26401 tgtttatcgg gattcagctg ccggaggatg agattcgggc ggcgtttgcg gggttgagga
    26461 agtaagttcg ggaaagatca actttttgag agttccaccg caacattatt acaagtggaa
    26521 ctctttagat atgtcagacc aaccaaagct tcttcaattg cgttttcatt tt
//
LOCUS       DAOTRK010000062        25570 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.062, whole genome shotgun sequence.
ACCESSION   DAOTRK010000062 DAOTRK010000000
VERSION     DAOTRK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.062"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..572
                     /locus_tag="QIE41_002429"
     CDS             <1..572
                     /locus_tag="QIE41_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDS3531307.1"
                     /translation="QIKESHKESATNVTFDYKELAPIFEGVQKKQIEALSNQLEHIKS
                     FKTDYDSRLNKFARDFQYLSSAFSINRQDLLKNYQEIRASITDYDDLLREIKELTISR
                     DKSVDDKRTLFDKKRDNWNSQEVQGEVKALNEDIADSDNKIRSKLTIVQNHRRENQYK
                     ANKNISNAMLNILDWFERYPDIVKNITQA"
     gene            complement(709..1374)
                     /gene="yieH"
                     /locus_tag="QIE41_002430"
     CDS             complement(709..1374)
                     /gene="yieH"
                     /locus_tag="QIE41_002430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDS3531308.1"
                     /translation="MTQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDQEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELADIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPELMFHAAKAMNVNVE
                     NCILVDNSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELGQLPALWKARSWN
                     ITR"
     gene            1541..2878
                     /gene="adeP"
                     /locus_tag="QIE41_002431"
     CDS             1541..2878
                     /gene="adeP"
                     /locus_tag="QIE41_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDS3531309.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTECVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(2932..3498)
                     /gene="yieF"
                     /locus_tag="QIE41_002432"
                     /gene_synonym="chrR"
     CDS             complement(2932..3498)
                     /gene="yieF"
                     /locus_tag="QIE41_002432"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDS3531310.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKS"
     gene            complement(3520..4269)
                     /locus_tag="QIE41_002433"
     CDS             complement(3520..4269)
                     /locus_tag="QIE41_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDS3531311.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            complement(4426..5385)
                     /gene="yidZ"
                     /locus_tag="QIE41_002434"
     CDS             complement(4426..5385)
                     /gene="yidZ"
                     /locus_tag="QIE41_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDS3531312.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKHLYASMA"
     gene            complement(5360..6535)
                     /gene="mdtL"
                     /locus_tag="QIE41_002435"
     CDS             complement(5360..6535)
                     /gene="mdtL"
                     /locus_tag="QIE41_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDS3531313.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTNSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(6668..7915)
                     /gene="tnaB"
                     /locus_tag="QIE41_002436"
     CDS             complement(6668..7915)
                     /gene="tnaB"
                     /locus_tag="QIE41_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDS3531314.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(8007..9422)
                     /gene="tnaA"
                     /locus_tag="QIE41_002437"
     CDS             complement(8007..9422)
                     /gene="tnaA"
                     /locus_tag="QIE41_002437"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDS3531315.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(9643..9717)
                     /gene="tnaC"
                     /locus_tag="QIE41_002438"
     CDS             complement(9643..9717)
                     /gene="tnaC"
                     /locus_tag="QIE41_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDS3531316.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            10117..12285
                     /locus_tag="QIE41_002439"
     CDS             10117..12285
                     /locus_tag="QIE41_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HDS3531317.1"
                     /translation="MVDNVTVGSVCAQSPSFMPELDGGKNKTQLIVDDIVAYLKNPSV
                     YSLEKEGPLNYFFNNCSEIEPWVLIGGGLIIIVSTSEQDPESLIVTVGDIEGKYATQI
                     PVPPSLTTSLKDIFIRPHTNADDESFTKEQKKANSKYDIVDYNCKVYFNEKPEDLVVC
                     RHISLQYCIDSMNENTGKVPLKECYSTPEGIQQHFPYELDQQFDNLINNPPPGTCVVA
                     SDKFGEILSVFFHRMEKEKLTHMAAIVKSQKHAMAVRLRIKQTPAGETEYVVSFYDPN
                     ATNTAVRYKAKNCDSFGSLQSFINIELANIKWVKTEICSECVGIIPYLPREQAHLLSG
                     IDNELQPPLSPSALYLLMQMGTYENIVIFFDKLRNSQEMTVSKVLEILAAKGPGGTYG
                     LCMSLQHDNLNKINEYIKNLKELARKFNFSREDLETLLLAKDNRGVSAISVALSKNKK
                     ESVKVLFLAIDEFEKEFGVNKNEMLFYIGKKTCSTYDLNSAIKTNNYNVVNILLANIK
                     ARMFKNEINKEDILKLMAAREWAGESDKWTKASGLYSAIVKGYTEIVAAWMETADVIA
                     SHYENDKDVVRELLSLSRNNAVCSLHIASFKKMSKQVIDVYLNAAIRLALQHGFTFDE
                     IVEQFTRDFDGKPFSHVVNNGDDIHMGLWLKIFKIVVGENENYLKDVMMQLEEKNNEG
                     KSVISQANGNPVLKELFWKAVDEFNFPQEELNRLKQYRSL"
     gene            complement(12434..13798)
                     /gene="mnmE"
                     /locus_tag="QIE41_002440"
     CDS             complement(12434..13798)
                     /gene="mnmE"
                     /locus_tag="QIE41_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDS3531318.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(13904..15550)
                     /gene="yidC"
                     /locus_tag="QIE41_002441"
     CDS             complement(13904..15550)
                     /gene="yidC"
                     /locus_tag="QIE41_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDS3531319.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(15553..15810)
                     /gene="yidD"
                     /locus_tag="QIE41_002442"
     CDS             complement(15553..15810)
                     /gene="yidD"
                     /locus_tag="QIE41_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDS3531320.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(15774..16133)
                     /gene="rnpA"
                     /locus_tag="QIE41_002443"
     CDS             complement(15774..16133)
                     /gene="rnpA"
                     /locus_tag="QIE41_002443"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDS3531321.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(16150..16290)
                     /gene="rpmH"
                     /locus_tag="QIE41_002444"
     CDS             complement(16150..16290)
                     /gene="rpmH"
                     /locus_tag="QIE41_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDS3531322.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(16520..16600)
                     /locus_tag="QIE41_002445"
     CDS             complement(16520..16600)
                     /locus_tag="QIE41_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531323.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            16897..18300
                     /gene="dnaA"
                     /locus_tag="QIE41_002446"
     CDS             16897..18300
                     /gene="dnaA"
                     /locus_tag="QIE41_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDS3531324.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            18305..19405
                     /gene="dnaN"
                     /locus_tag="QIE41_002447"
     CDS             18305..19405
                     /gene="dnaN"
                     /locus_tag="QIE41_002447"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDS3531325.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            19405..20478
                     /gene="recF"
                     /locus_tag="QIE41_002448"
     CDS             19405..20478
                     /gene="recF"
                     /locus_tag="QIE41_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDS3531326.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            20507..22921
                     /gene="gyrB"
                     /locus_tag="QIE41_002449"
     CDS             20507..22921
                     /gene="gyrB"
                     /locus_tag="QIE41_002449"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDS3531327.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            23161..23559
                     /locus_tag="QIE41_002450"
     CDS             23161..23559
                     /locus_tag="QIE41_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDS3531328.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVTTQANNDLLSAGMELLKGKLFR"
     gene            23674..24486
                     /gene="yidA"
                     /locus_tag="QIE41_002451"
     CDS             23674..24486
                     /gene="yidA"
                     /locus_tag="QIE41_002451"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDS3531329.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(24530..25186)
                     /locus_tag="QIE41_002452"
     CDS             complement(24530..25186)
                     /locus_tag="QIE41_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531330.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDKVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLTGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            25464..>25570
                     /locus_tag="QIE41_002453"
     CDS             25464..>25570
                     /locus_tag="QIE41_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS3531331.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELC"
BASE COUNT         6638 a         6223 c         6519 g         6190 t
ORIGIN      
        1 agcaaataaa ggagtctcat aaagaatctg ctacaaatgt aacatttgat tataaggaac
       61 ttgcacccat ttttgaaggt gttcaaaaaa aacaaattga agcgctatca aaccaactgg
      121 aacatataaa atcttttaag acagattacg attcaaggct caataagttt gctcgcgatt
      181 ttcaatattt atccagtgca ttttcaatta acaggcagga tttattaaaa aactatcagg
      241 aaataagagc ttccattaca gattacgatg acttattgcg agaaattaaa gagttgacca
      301 taagtcggga taaatcagta gatgataaaa ggactttatt tgataaaaaa agggacaact
      361 ggaatagtca agaagtacag ggtgaagtaa aggcattaaa tgaggatatt gctgactctg
      421 acaataagat acgttctaag cttactattg ttcaaaatca tagacgcgag aatcaatata
      481 aagcgaataa aaatatcagt aacgcgatgc ttaatattct ggattggttc gaacggtatc
      541 ccgatattgt aaaaaatata acccaggctt aactatgctg agggtggatg acaggacgaa
      601 taacgacata aattattcat agggaataga cgcttactca tcagacacct ggtattcttt
      661 atctcaaacc agcgtgactg atgtgtaagc gttgaagtaa ggaggcgatt atcgcgttat
      721 attccagctg cgtgccttcc agagcgcagg cagctgcccc agctcggtaa aggtggtgac
      781 tttcggatga acgatcggct tattgtgcgg gtcagcgcag aagtaaaaca cttccatacc
      841 ggcatcgatt cctgattgcg caccggcgct ggagttatcg accagaatgc agttttcaac
      901 gttcacattc atcgcttttg ccgcatggaa cattaactcc gggtcgggct tccaacgctg
      961 aatatcgtag ccgctgaaca gtttttccgg gaagtagtgc agcatattga gcttaccaag
     1021 cgaatgctgc attttgctga ccggaccgtt agagacgacg cacatcggca cgttaatgct
     1081 cgccagcaat tcgttcgctc ctgcgatgtc cgcaagttcg gtatcaaaca gacgtgctac
     1141 ttctgcgcgg taaacgtgct cggcatcggc ctggaccatc gtcacgccct gttctttact
     1201 gatgatgtcg atgatttcgt agagttttac gcctttaaaa cgggtaaaaa tctcttcctg
     1261 gtcaacgtga atgccaaatt ccctgaacat cgtgacatag gcgcgagagc aaatgacctc
     1321 gctatcgacc agcgtaccgt cgcagtcgaa aaataccgct tctatctggg tcatgtcttt
     1381 cctttatcgt tagtttaacg ttttcgtcat gccaattcgc atcacgcaat cgttgccgta
     1441 taagcaaata caatgaaaaa aagttatctt cagccgtcca tattgtctca ttttggtata
     1501 cgatagcgac ggattttccc tccttgttcg gaaatagata atgagtcaac aacacacaac
     1561 ccaggcttct ggccagggga tgctggaacg cgtgtttaaa ctgcgcgaac atggcacgac
     1621 ggcacggacc gaagtgatcg ccggttttac caccttcctg acgatggttt acatcgtttt
     1681 tgttaacccg caaattcttg gcgttgctgg catggatacc agcgccgtct tcgttactac
     1741 ctgtctgatt gctgcattcg gcagtattat gatgggactg tttgctaacc tgccagttgc
     1801 gctggcacct gctatgggcc tgaatgcgtt cttcgctttt gtggttgtac aggcgatggg
     1861 cttgccgtgg caagtcggga tgggcgcaat cttctggggc gcgataggtc tgctgttact
     1921 gacgattttc cgcgttcgct actggatgat agccaacatt ccggtgagtc tgcgtgtggg
     1981 cattaccagc ggtatcggcc tgtttattgg catgatggga ctgaagaatg caggtgtgat
     2041 tgtcgctaac ccggaaacgc tggtgagcat cggtaatctg acttctcaca gcgtactgct
     2101 ggggatcctc ggcttcttca tcattgctat tctggcctca cgcaacattc acgcggcggt
     2161 gctggtttcc atcgtggtga cgacgctgct gggctggatg ctgggtgatg tccactacaa
     2221 tggcatcgtt tctgcgccgc cgagcgtaat gactgtcgtg ggtcatgtag atttagccgg
     2281 gtcgtttaac cttgggctgg caggggtgat tttctctttc atgctggtca acttgtttga
     2341 ctcctccggt acgctgattg gcgtgacaga taaagcagga ctggcggatg aaaaaggtaa
     2401 attcccgcgc atgaagcagg cactgtatgt cgacagcatc tcttccgtga ccggttcgtt
     2461 tatcgggact tcttctgtta cggcgtatat cgagtcttct tccggcgttt ccgttggggg
     2521 gcgtaccggc ctgacggcag tcgttgttgg tctgctgttc ctgctggtta tcttcctgtc
     2581 gccgctggcg gggatggtgc cgggatatgc cgcagctggc gcgctgatct acgtgggcgt
     2641 actgatgacc tcaagtcttg ctcgcgtgaa ctggcaggat cttactgaat gtgttccggc
     2701 gtttattacc gctgtgatga tgccgttcag cttctcgatt accgaaggta ttgcgctggg
     2761 ctttatctcc tactgcgtga tgaagattgg taccggacgt ctgcgtgatc taagcccgtg
     2821 cgtaatcatc gttgcgctgc tgtttatcct gaagattgtg tttatcgacg ctcactaaaa
     2881 tcgatagcgc gttaaaaacc cgccatgcat gtttgctggc gggtttttta tttagctctt
     2941 aactcgctga ataaactcac caaatgcagt caattgcccg gtcaggtggt ccagcgtacc
     3001 ctgatcaatc acttctccgg tttgcggatc aactttgttc tgaatcacgc cgcccataaa
     3061 ttccggcttg ttcatcacca ttgcatcgag gaaaaccaga atctggcgca ggtgatactg
     3121 acagcgcgcg ccgccaatca cgcccattga gctggtctga atcaacaccg gtttaccggc
     3181 cagaggttga tccggcaggc gggaaagcca gtcgatggca tttttcagcc cacccggtac
     3241 cgagtagtta tattccggcg tgacgatcac cacaccgtca gcctgacgga tctgttccgc
     3301 cagagcttca accgttgctg gaaaaccttc ttcctgctgt acgtcagcgt catacaaggg
     3361 aatgtcggca atggaaggta acgcattgac ttccatgctc gccggagcaa ttttcggcag
     3421 ggtacgtgca accatgccat taaatgagcc tttgcgcagg ctccccagta acgtaaccac
     3481 ctgcaatttt tcagacatga tgactcctgt ggttgtctat caatttggcg aaaaagcctt
     3541 cacggtagag agctgggcaa aacgcatcat ccggctggta ggattgttgg cgcggctgtg
     3601 aatatcgggc aggcttttcc agccgtgttt gccatctaac tcccagacac tgagcttttc
     3661 aatcgtgggc gtgaccgcca cggaccacac cagcacgtcc tccgcgtcgc ataacgcttc
     3721 tacatgattt acatgagcac atttcaggcg tccggcaatg ggcagcagct gcagatcgcg
     3781 cggatcgtca ttcaaaacat caccggttag ttttagcacg cttcggcgca gcgtgatgag
     3841 ctgcgcccgc gcttcgtttg gatcattttg tttactgatc catagcgatt cattgctgga
     3901 aaaggtgtgg ttatcgggcg cgtgtggact atgaaacccg cgcgtcgcac gctgcagttc
     3961 catatcgagg ccacattcac cttcggttgt taacgccacg ccaaccatat tcccggcata
     4021 ggaaatagaa aacgaaggca aatttttatc gctgaaaacc ggtttacctt tcggcagggt
     4081 gacgatttcc ggcaattcgc caatgccata cagcatgaac atcagttctg cgagtaaacc
     4141 tctggacgcc agaaaacggc tttgacgatg agggggaatg tttcgggctt cttgatgaca
     4201 ctgggagggg agacgaacag aaatcagatg tccttccgtt aaaatccctc gggcaaagtg
     4261 agtcgccatt tttcgctcca tgcttaatgg tccattatga atacaagagt aatcatttac
     4321 tggaatgatg gacagactaa agcggctaaa agttcgattc agacaagctt attacaactt
     4381 ttttacattt tattgtgaaa ttgggtcgcg tttcatacga ttcggttatg ccatcgacgc
     4441 gtaaagatgt ttaatggttt cccgtaacca gacgatcttc ggattatggc tgttccgttt
     4501 atgccacagc agggtaaaag ggacttccag ctttttttgc tggctttcgt caaacgggag
     4561 aggaagagca accaacggca gttggtggag ttgattgtag tactgacagt agcgcggtgc
     4621 ggtcgccagt agcagattgt cgggctgtgc tgccataaac agtgactgct cgaattccgg
     4681 caggctcata gcaatcgtgc gttcgcgccc cagctcctgt aacacattgt ccagcgccca
     4741 ggtatcgctc tgttcccagc aaatgctgat atgcggataa cgtaaaaagg tgtccagatt
     4801 ccacgtttca tgcagtgccg gatgatcttt tcgtaaccag acgcagggca catcactaaa
     4861 cagcacttca taatcaatgg ctaacggtag cgagcttaac agctcccgcg aacggggatg
     4921 actttcgcga ccggaaaaac caatatccac ttcaccacga gtaatggcat ctaaggaatc
     4981 gtaatcccag ttacgtaatt tgatggtcgc ctgcgggtaa cgttggtaga tccgtttcga
     5041 cagcgcatta agcatgatca tcatcagcgg cgattccgcc gccagctcaa acttcaggcc
     5101 gcgcggtgtc tggtgatgcg gtttatccag cagcaggttg ctcatttgca tccactccgc
     5161 cagattttgc tccatgctga ccattagcgg tgtgggcgac agccccagcg gtgagttcac
     5221 aaagagcggg tcgtcaaacc acgctcttaa ctttgccagc gacttactca ccgccgaagg
     5281 ggtcacgttc atccgcttcg ccgctttggt cacgctgcgc tcctgcatca gcagttgcag
     5341 acagagcagc agattgagat caagcgtggt gatggatttc ttcatgagcg gcaaccgggc
     5401 gtccaggcgc gacgaacata atcagcaaca ggctcactat gctacaggca atcagaatcc
     5461 cgatcagcat attccatgcg ccgataccaa ccaccgctgc cagccaaatc cacagtgacg
     5521 aaccgcaaac ctgcgcaata cctaaggtcg agctggctac gcccgcgcgt aatgaaaacg
     5581 gccctaacgc ctgactcatc gctacgccaa aacctaccga gaaaccggcg caaatcagcg
     5641 tgataccaaa cagagaaacc gcatgggaag gtgaaacggc aagggtgatg cccgccgcca
     5701 ggaataatac ctgcgaggtg atcatcaacg tacgtggctt aaaaattccc agcgcaaatg
     5761 gcgtggagaa tgaaacggtc atgctgacgc cagcggtcaa cgccataatg gtggcgtatt
     5821 ctccgcgctc aaaacccatg atttccatca gcaataccgg cgacgtgttg acgaaggtga
     5881 ggatcaccga aacgctgagg gtggtgataa caacacggct gaggaaaaac cggttaagca
     5941 gcgactcgct attttctcgt ggtttgtccg aatttgtggg ggccgcaggg cgcgtttctt
     6001 ttaaaataaa caaagacaac atcagtaccg cgatgcccat cgttgccatc gtccagaaca
     6061 gactctgcca cgggaattta agcataatca ggtgtccgag cactggcgct aacaccggaa
     6121 tgatgcaggt aatgccgttg agtaacgaca gcactttagc ccgacgtcga tcatccagtg
     6181 tgtcgcgtaa aatggcgaac gcaaccacgt aacaacagcc cgcgcccagc ccctgtagaa
     6241 agcgccctgc aagaaataac gtgctggttt cagccagtga acagaacacc gaggcgataa
     6301 taaatagcgc cgcgccgggt atggcgacag gctttctccc tgaacgatcg gccactttac
     6361 cggcaaataa catcgcagct gccatgcccg ccagataaac ggagaacgca atatgcaact
     6421 gcgcttcgct ggcattgaga tcggcggcga tgcgcggtaa accaacgagg tacatatcaa
     6481 tcccggcggg ataaagtaaa accagggcaa aactacaaat caaaaagcgg gacatgggct
     6541 aaagcgtcct gatagtaagg tgacgcaaag catacgtggt gaaggagaat caggcgagtt
     6601 gccatttgga cataagtgat ttcctgacag gcaataagcc agcgaaatgg ctggcttctt
     6661 gaagggatca gccaaattta ggtaacacgt taaagacgtt accgaaccag cataaaatta
     6721 cggttatacc aaagagaatg acaatcgccg gaataagatt gccgccccag accgtgaaca
     6781 tctgattggg aaacttcttg cgagctttga ttgccagcac cgcggggata atgaccgccc
     6841 agatggtggc acacagtccg gcaccgccga tcccgtaaat gaagccgttc gggaagatca
     6901 gatacaacaa cgcaggtggc aggaaggtta acagcacggt tttgaaacgc ccgccgtggg
     6961 agttatcaat cttaaacaga tccgccagat aatcgaacaa ccccagcgtg acaccaaaga
     7021 acgaactggc aacagctaag ttggagaaca ccagcaggca aaactcgata atgccgtgct
     7081 gtttggtgcc gaggaacgat ttgaccagcg aatcaacgtt gccgcctgag gagattatcg
     7141 ccttaaagct ttctcgtggg atattcccca tcgtgcaata gagccagaag agataaatca
     7201 ccagcgccag cagcgaaccg aagaccacgc ttttgattag cttatctttg cgtttgccat
     7261 agcaaataat caggctggga atattgccgt ggaaaccaaa tgacgccaga cacaccggca
     7321 aagccataaa gatatacggg aaataagatg tacccgcagt ggtgctggtg gaatcgcgca
     7381 gaatggagta atcgacctgg aagaagaaag aaccaaaaac aattacaaag gagataatct
     7441 tcagcccaag gaacaatgag gtaatacgac tggcggctaa cgagcttatc cacaatacgc
     7501 tggcaacgaa aatggctgtg cagatcccga caatacgtgg attagcgtga taacccaaat
     7561 tcattgatat cgtttcacta atgatcgcac cattagcaga gatataggca taagtgagga
     7621 tatagagaac gaaggcaacg gtaataccgc tgataatgtt ccaggtgtta ccgattaaat
     7681 ctttggtgat ggtgttaaaa ctggagccga cgggataatt taaatttgct tctaataaca
     7741 acaacccgga atgaagcatt gaaaaccagg caatgataag gataaaggca ccccagaaaa
     7801 accaggcacc ggcaagatca acaggtaaag caaacatacc tccaccaatt actgtacctg
     7861 ctataaccat aacaccccaa aatgcagagt gctttttttc agcttgatca gtcatgatgc
     7921 cacctttaga ggaaggctat ttttgttatt gaggatgtag ggtaagagag tggctaacat
     7981 ccttatagcc actctgaatg ttttaattaa acttctttca gttttgcggt gaagtgacgc
     8041 aatactttcg gttcgtaggt aaaggttaac cctttaatat tcgccgcgtt ctctttcaca
     8101 tgtttaaagg cttcaataat gaagtccata tgtgtttgag tatatgttgc acgcggaatg
     8161 gttaaacgca gcagttcagc cgggcatggc agttgtttac cggttttcgg atcgcggcct
     8221 aacaggaaag aaccaatttc taccgcacgg ataccggcga ctttatacag ctcgcacgcc
     8281 agcgcctgtg ccgggaactg gtctgccggg atatgcggca gcagtttacc ggcatcaacg
     8341 aatgccgcgt gaccgcccgc ctgctggcag acaacgccaa tctcttccag accatcgacc
     8401 agatactgta cctgcgcgat acgataagcc agccagtcca gattcatgcc gtcatacaga
     8461 cctaccgcca gacgctccat cgcgccgcct tccaggccgc catatgtcgg gaagccttcc
     8521 tgtaccacgc aaagggttct gcattcggtg tacacatcaa agaagctgtc gtctttcatg
     8581 cacagcaagc cgcccatcgg caccatcgca tctttcttgg cggacatcgc cagcatatcg
     8641 gcatatttgt aggtttcgcg ggtgatctgc tcgatggtcc agtctttgta ttctgcttca
     8701 cgctgcttga tgaaataggc gttttcagca aagcgtgcgg agtccattac caccggaata
     8761 tcgtatttct tcgcgatgct gtacatcgct tttaagtttg ccagtgaaac cggctgacca
     8821 cctgcagagt tactggtgat ggtcgcaacg atatacggca cgttattcgg gccaacttct
     8881 tcaatgccgc gttctaatcc ctcaaggtca aagttgcctt taaagtcgta acgcacgccc
     8941 gtatcgaagg cttctttgat atagacgtta cgcacggtac agccgttgat ctggctatgg
     9001 ccctgcgtgg tatcaaagaa atagttagag aacgccacca ttttgctgcg atccaggcct
     9061 ttttcctgct cgcgtttttt aatcagtacc ggaatataga tttgttctgc accacggccc
     9121 tggtgagtcg gaatagtgta ttgataacca aagatatttt ttactgactc ggctaacgca
     9181 tagtagctgc ggctgccgct gtaagcttcg tcgccgcgca tcatcgcggc ctgcatactc
     9241 tgcgtcaccg ccccggtgcc gctgtcggtc agtaaatcga taaacacatc ttcgctatcc
     9301 agcaggaacg ggttcatacc ggatttaata attgcttctt cacgataagc gcgagtggta
     9361 cgttttactg gctcaataac acggatgcgg aacggttcag ggagatgttt aaagttttcc
     9421 attacataat ccttcattta ttttaattac agtgatccct gtgaatatta catctgcaat
     9481 atccattaat tcgccagata tatatgcaag caccatcatt tactaaaaca agtaaataaa
     9541 cgagagatgg cctttgcaaa aggcaaaatt aagatatagc aaacaaatag atcacattga
     9601 atctaatcgg tttggctctg gtgatggcta cagaagggca aatcaagggc gatgatcaac
     9661 aatcttgttg tcaatattga accattttga ggtcacacat atatgtaaga tattcataat
     9721 gcacttatcc tcgaaagaca cgaggctaca gtataatact caagaaattt gtctattctg
     9781 aaattgttta aatgtgaatc gaatcacaat tagatattgg atgtgtttgt gatagctgcc
     9841 ccatgcaaaa tattagcaaa aacacatgaa tggataaatt ttttaatttt tatcattatt
     9901 gttgaaggtg atggcattga ttataggggt tccattacaa ggagcggttt ccatcgttga
     9961 cgtcgcggtt tactcacttc attaccaggt tgatgacctg atggggtaat gacgtaaaaa
    10021 gacaaataac tctataaatg agcgaaattt gatagttggt ttttaaatag taaccttatt
    10081 attactacaa ctttcatatg aatgagggga aagaaaatgg ttgataacgt aactgttggc
    10141 agtgtatgtg cacaatctcc ttctttcatg cctgagttgg atggaggaaa aaataaaact
    10201 caattaatcg ttgacgatat agtggcgtat cttaaaaatc cttcagttta ttcacttgaa
    10261 aaagagggtc cgttaaatta tttttttaat aattgtagcg agattgaacc atgggtttta
    10321 attggtggtg gacttattat tattgtctcc acatctgagc aagaccctga aagtctgatt
    10381 gtaactgtgg gggatattga aggaaagtat gctacacaga ttcccgtacc accctcgctt
    10441 acaacatcac tcaaagatat ttttattcgt cctcatacga acgcagatga tgagagtttt
    10501 actaaagagc agaaaaaagc taatagtaaa tatgatattg tagattataa ctgtaaggtt
    10561 tattttaatg aaaaaccaga agatttagtc gtgtgcaggc atatttcgct gcagtattgt
    10621 atagattcaa tgaatgaaaa tacaggtaaa gttccattaa aggaatgcta ttcaactcca
    10681 gaagggatac agcaacattt tccatacgaa cttgatcagc aatttgataa tctaataaac
    10741 aatccaccac caggtacatg cgtcgttgcc agtgacaagt tcggggagat attatctgtc
    10801 ttctttcaca gaatggaaaa agagaagtta acgcatatgg cggcaatcgt taaatctcaa
    10861 aaacatgcta tggccgtccg cttgaggata aaacaaacgc ctgccggaga aactgaatat
    10921 gttgtatcct tttatgatcc taatgcaacc aatactgcag tacgctataa agcaaaaaac
    10981 tgtgattctt ttgggtcatt acaatcattt ataaatattg agttggcgaa tataaaatgg
    11041 gtaaaaacag agatttgctc cgagtgtgta ggcataattc cttatctccc tcgagagcag
    11101 gcccatttat taagtggtat agacaatgaa ttgcaaccac cattatcacc atcagcatta
    11161 tatctattaa tgcaaatggg gacgtatgaa aatattgtta tttttttcga taagttaaga
    11221 aattcccaag aaatgacagt atcaaaggtt cttgagattc ttgctgctaa aggaccaggg
    11281 ggaacatatg ggttatgtat gtcactgcaa cacgataatc ttaataagat taatgaatat
    11341 ataaaaaatt taaaagagtt ggccagaaaa tttaacttta gccgggaaga cctggaaact
    11401 ctactccttg cgaaagataa ccgtggtgtg agcgcgattt ctgtagcttt atcaaaaaat
    11461 aaaaaagaaa gtgtcaaggt attgttcttg gcgatagatg aatttgagaa agagtttggg
    11521 gtaaataaaa atgaaatgct tttttatata gggaaaaaaa catgctcaac ttatgattta
    11581 aatagcgcta ttaaaaccaa taattataat gttgttaata tattgctcgc caatataaaa
    11641 gcccggatgt ttaaaaatga aataaataaa gaagatatat tgaaactgat ggcagcaaga
    11701 gaatgggcgg gggagtcaga caaatggacg aaggcatcag gcttatattc tgcgatagtg
    11761 aaagggtata cggagattgt tgccgcctgg atggagacag ctgatgtgat agccagccat
    11821 tatgaaaatg ataaagatgt ggtgagagaa ctactgtcgc tgagcagaaa taatgccgtc
    11881 tgctctttgc atattgcaag ctttaagaaa atgagtaaac aggtcattga cgtatatctg
    11941 aatgcggcga ttcgcctggc gttgcaacac gggttcactt tcgatgaaat tgtggagcag
    12001 tttacccgtg actttgatgg caagccattc tctcatgtgg taaataacgg ggatgatata
    12061 catatgggac tctggctgaa aatattcaaa attgtggttg gtgaaaatga aaattacctt
    12121 aaagatgtga tgatgcagct ggaagaaaag aataatgaag gtaaatctgt aataagtcag
    12181 gcaaatggca atcctgtact taaagaacta ttctggaaag cagtagacga atttaacttt
    12241 ccgcaagagg agcttaatag gttaaagcaa tatcgttctc tgtaaagaca ggactgcaag
    12301 gtgtttaaag tcgctccagc cagtagcaaa tgcagttttg gtgcaggatg ctcataaaaa
    12361 aacaagcgct gttccgttct gatagttagt ttacccagag cggaacagtg ccctttatat
    12421 caatttagac gacttactta ccaatacaga agctggagaa aatccgcccc agcaggtcgt
    12481 cagaggtaaa ctccccggtg atctcgctta agttctgctg cgccaggcgc agctcttccg
    12541 ccagcaattc acctgcccat gcacccaaca gttgtgcttt gccctgttgc agatgctccg
    12601 ccgcttgttc cagcgcctgt aggtgacgac gacgcgccag gaagccgcct tccatgttgg
    12661 tgtcaaagcc catgctctgt ttgagatggt tacgcagcac gtccacgcct tcgccagtcc
    12721 ttgccgagag acgaatcaac gcatgaccgt tcacttcact cattcccagc gtttcgccgg
    12781 tgatatcggc tttattgcgc accacggtga tcggcagttt cgcgggcaga cgggcgataa
    12841 attccggcca gatctctgcc ggatcaacgg cgtctgtcgt ggtgccatcg accataaaca
    12901 gcacgcggtc ggcctgttca atttcctgcc acgcgcgctc gataccaata cgttctactt
    12961 cgtcactggc ttcacgtagt ccggcggtat cgatgatatg cagcggcatt ccgtcaatgt
    13021 ggatatgctc acgcagcacg tcacgcgtgg ttccggcgat atcggttacg attgccgctt
    13081 cacggcccgc cagcgcgttt aacaggctcg atttaccggc gttaggacgt ccagcaatca
    13141 ctactttcat cccttcgcgc aacaaactac cctgacgtgc ttcagcacgc actgcatcaa
    13201 gatcggcaat tacgtcattg agctgggctt caattttgcc gtcggagagg aaatcgatct
    13261 cttcatcagg gaaatcaatt gccgcttcga cgtaaatgcg caagtgggtg agggcttcca
    13321 ccagatggtt gacccgtgcg gagaatgcgc cttgcagcga gtttagcgcc gaacgggccg
    13381 cctgttccga gctggcgtcg ataagatccg caatcgcctc agcctgggct aagtcgagtt
    13441 tatcgttaag aaacgcgcgt tcggaaaact caccagggcg ggcaatccgc aggccgggaa
    13501 tggtcagaat gcgttttaac agcaggtcga ggatcaccgg accgccatga ccctgcagtt
    13561 caagcacatc ttcgccggtg aacgagttcg ggccagggaa ccatagcgca atcccctgat
    13621 cgagcacgct gccgtcggcg tctttaaacg gaagataatc ggcgtagcgc ggcttaggca
    13681 gtttacccag cacggtttcg gcaacttcac gggctttgag gccggagatg cgcaggatgc
    13741 caacgccgcc acgtcccgga ggcgtggcct gggctacgat agtgtcatta tcgctcatga
    13801 tgttcctgtt gctttgtgtg gcggatgcgc ggtgcttatc cgccctacga aaagaaaaaa
    13861 ggcggtcaac tgaccgccct tattttagcg aaaactcacc gaatcaggat tttttcttct
    13921 cgcggctatg caggccacgt ttttccagac cacggtaaat cagctgctgc tgaataatgg
    13981 ttaccaggtt gctgacgata tagtacagca ccagacctga cgggaaccac aggaagaaca
    14041 cggtgaagat gaccggcata aaggtcatga tcttctgctg catcgggtcg gtcacggtgg
    14101 tcggcgacat cttctgaatg aagaacatcg ttacgcccat caggatcggc aggatgtagt
    14161 acgggtcctg tgcagacagg tcgtggatcc acagtgcaaa cggtgcctga cgcaattcaa
    14221 cggaacccat cagcatgtag tacaacgcca ggaagattgg catctggatc agcagcggga
    14281 agcagccgcc cagcgggtta accttctcag ctttgtacag cgccatcatt tcctgactga
    14341 tacgctgttt gtcatcgccc agacgctcac gcattgcctg aatcttcggc tgcagcatac
    14401 gcatcttcgc catggaggtg tactgcgctt tggtcagcgg gtacatgatg ccacgaacga
    14461 taaaggtgat gatgataatg gagaagcccc agttacccac aaagctatgg atccatttca
    14521 gcagtttgaa cagcggctga gagatgaacc acaaccaacc gtaatcaacg gtcagatcca
    14581 ggtgcggagc aacagctgcc attttgtcct ggatttccgg gccaacccac agggtgctgt
    14641 tcatcgcgcc agtctgacca ggctgaacca gtaccggttg agacttgtag ccgatagcgg
    14701 cgatgccgtt acccagatta gcggtataga agttattggt accgtcgtta tgcgggatcc
    14761 acgccgtcgc gaaatactgt tgcagcattg ccacccaacc acctttcgaa gagatgttca
    14821 ggttttcgtt atcggcaatg gtatcgaact tgtatttctc gtacttctcg tcaggcgtgg
    14881 agtacgccgc gccacggaag gtgtgcagtg cgaagttgct gcttccggta tcgagatgcg
    14941 gtggcagagt gatggattgc ttcaactgac caaaggtgga gatctccagc ggtttctcgc
    15001 cagcgttctg cacgttgtag ttgacgttga cagcgtaatc accacgtttc aggacaaacg
    15061 ttttggtaaa cgtgttgcct gccgcgtcgg tatacgtcat cggcacctgc agttcgtttt
    15121 gaccttcagc cagcacataa gcgtcttttt caacgttata cagcggacgc gggccgttag
    15181 ccggattatc cgggccatca cgaccggtca gaccgctctg tgcctgataa ataaactgcg
    15241 gtgaagtttc cagcagctgg aacggctggg tagagttcag ctctttcggg taagcaggca
    15301 gcagagcttg ctcaacatca ccaccacggg tgttgatggt cagatcaagc acgtcggtct
    15361 taaccgagat cagtttcccc tggccactgg ccggtacgcc ctggtcggcg gcgctacccg
    15421 ctgcggtggt cgttgtctgc gtggtctgtt gggcctgagg ttgcgggttt ttatcctgct
    15481 cccaggcttg ccagatcatg aaagacacga acagcaaagc gatgactaaa agattgcgtt
    15541 gcgaatccat cgttagtgtt ctctggtatc aaatggtccg ggcgggacgg gatcgtcacc
    15601 accagggtgt aaagggtggc attttaatac gcgtttcacc gtcaaccaac tgccttttat
    15661 cactccaaac ctgcgcaatg cctcaattcc gtagcttgaa caggttggag tgaaacgaca
    15721 atgcggcccg agtagcggac taatcaggcg ttgatagacc cgaatgaggg ctatcaggac
    15781 ccgcgagcca ggcgacagtg gcggcgccat aatttttcca acgcttccga gagagcacgg
    15841 ttatcgaggt cggcaacccc ttttttcgcc accaccacga aatccatagc cgggagttca
    15901 tgttggcgca gacggaagct ttcacgcgtc agacgtttaa tccgattgcg ttcatgggcg
    15961 cgtcgaacgt ttttcttggc gactgtaaga ccgatacggg gatgccccag cgaattcagg
    16021 cggccgagaa tggtaatttg cggcgtgcca gcccgttgtg gctgctggaa gacgaatgtg
    16081 aattgactgg gagttaacaa gcgtaactcc ctgggaaatg cgagcttaac cactcagggg
    16141 ttagctttat tacttagaaa cggtcagacg agcgcggcct ttagcacgac gacgtgccag
    16201 aacctgacga ccatttttag tagccatacg agcacggaag ccgtgagaac ggttgcgctt
    16261 cagtacagac ggttgaaaag tgcgtttcat ggcgatttct acctaaactt gaataaattc
    16321 aatggcttta ttggatatcc gccgaaaaat gaaacgatgg acaccgaagc catgggtgat
    16381 taaagaggcc ggattgtaat aattgtacac tccggagtca attctctttc cttatttacc
    16441 gcgcttttcc gcaccttttc gcagggaaaa tgtacgacct cacaccagtg aaaaccagca
    16501 tggcgcgccg ggtggaggat tatacgggct gacgggtaaa gcgcaaggat cgtcctggat
    16561 ctttattaga tcgattaagc caatttttgt ctatggtcat taaattttcc aatatgcggc
    16621 gtaaatcgtg cccgcctcgc ggcaggatcg tttacactta gcgagttctg gaaagtcctg
    16681 tggataaatc gggaaaatct gtgagaaaca gaagatctct tgcgcagttt aggctatgat
    16741 ccgcggtccc gatcgttttg caggatcttg atcgggcata taaccgcaga cagcggttcg
    16801 tgcgtcaccc tcatgcaggg tcttttcgac gtacgtcaac aatcatgaat gtttcagcct
    16861 tagtcattat cgacttttgt tcgagtggag tccgccgtgt cactttcgct ttggcagcag
    16921 tgtcttgccc gattgcagga tgagttacca gccacagaat tcagtatgtg gatacgccca
    16981 ttgcaggcgg aactgagcga taacacgctg gccctgtacg cgccaaatcg ttttgtcctc
    17041 gattgggtac gggacaagta ccttaataat atcaatggac tgctaaccag tttctgcgga
    17101 gcggatgccc cacagctgcg ttttgaagtc ggcaccaaac cggtgacgca aacgccacaa
    17161 gcggcagtga cgagcaacgt cgcggcccct gcacaggtgg cgcaaacgca gccgcaacgt
    17221 gctgcgcctt ctacgcgctc aggttgggat aacgtcccgg ccccggcaga accgacctat
    17281 cgttctaacg taaacgtcaa acacacgttt gataacttcg ttgaaggtaa atctaaccaa
    17341 ctggcgcgcg cggcggctcg ccaggtggca gataaccctg gcggtgccta taacccgttg
    17401 ttcctttatg gcggcacggg tctgggtaaa actcacctgc tgcatgcggt gggtaacggc
    17461 attatggcgc gcaagccgaa tgccaaagtg gtttatatgc actccgagcg ctttgttcag
    17521 gacatggtta aagccctgca aaacaacgcg atcgaagagt ttaaacgcta ctaccgttcc
    17581 gtagatgcac tgctgatcga cgatattcag ttttttgcta ataaagaacg atctcaggaa
    17641 gagtttttcc acaccttcaa cgccctgctg gaaggtaatc aacagatcat tctcacctcg
    17701 gatcgctatc cgaaagagat caacggcgtt gaggatcgtt tgaaatcccg cttcggctgg
    17761 gggctgactg tggcgatcga accgccagag ctggaaaccc gcgtggcgat cctgatgaaa
    17821 aaggccgacg aaaacgacat tcgtttgccg ggcgaagtgg cgttctttat cgccaagcgt
    17881 ctacgatcta acgtacgtga gctggaaggc gcgctgaacc gcgttattgc taacgccaac
    17941 tttaccggac gggcgatcac catcgacttc gtgcgtgagg cgctgcgcga cttgctggca
    18001 ttgcaggaaa aactggtcac catcgacaat attcagaaga cggtggcgga gtactacaag
    18061 atcaaagtcg cggatctcct ttccaagcgt cgatcccgct cggtggcgcg tccgcgccag
    18121 atggcgatgg cgctggcgaa agagctgact aaccacagtc tgccggagat tggcgatgcg
    18181 tttggtggcc gtgaccacac gacggtgctt catgcctgcc gtaagatcga gcagctgcgt
    18241 gaagagagcc acgatatcaa agaagatttt tccaatttaa tcagaacatt gtcatcgtaa
    18301 acctatgaaa tttaccgtag aacgtgagca tttattaaaa ccgctacaac aggtgagcgg
    18361 gccgttaggt ggtcgtccta cgctaccgat tctcggtaat ctgctgttac aggttgctga
    18421 cggtacgttg tcgctgaccg gtactgatct cgagatggaa atggtggcac gcgttgcgct
    18481 ggttcagcca cacgaaccag gggcgacgac cgttccggcg cgcaaattct ttgatatctg
    18541 ccgtggtctg cctgaaggcg cggaaattgc cgtgcagctg gaaggtgagc gtatgctggt
    18601 gcgctccggg cgtagccgtt tttcgctgtc taccctgcca gccgcggatt tcccgaacct
    18661 cgatgactgg cagagcgaag tcgaatttac cctgccgcag gcgacgatga agcgtctgat
    18721 tgaagcgacc cagttttcta tggcgcatca ggacgttcgc tattacttaa acggtatgct
    18781 gtttgaaacc gaaggcgaag aactgcgcac cgtggcgacc gacggccacc gcctggcggt
    18841 ctgctccatg ccaattggtc aatctttgcc aagccattcg gtgatcgtgc cgcgtaaagg
    18901 cgtgattgaa ctgatgcgta tgctcgacgg cggcgacaat ccgctgcgcg tgcagattgg
    18961 cagcaacaac attcgcgccc acgttggcga ctttatcttc acctcgaaac tggtggatgg
    19021 tcgcttcccg gattaccgac gcgttctgcc gaagaaccca gacaaacatc tggaagcagg
    19081 ctgcgatctc ctcaagcagg cgtttgctcg cgcggcaatt ctctctaacg agaaattccg
    19141 cggtgtgcgc ctgtatgtca gcgaaaacca gctgaaaatc accgccaata acccggaaca
    19201 ggaagaagcg gaagagatcc tcgacgttac ctatagcggt gcggagatgg aaatcggctt
    19261 caacgtcagc tatgtgctgg atgttctgaa cgcgctgaaa tgtgaaaacg tgcgcatgat
    19321 gctgaccgat tcggtttcca gcgtgcagat tgaagatgcc gcatcacagt cggctgccta
    19381 tgttgtcatg ccaatgagac tgtaatgtcc ctcacccgct tgttgatccg cgatttccgc
    19441 aatatcgaaa ccgcggatct cgctttatct cccggcttta actttctggt aggtgccaac
    19501 ggcagtggca aaaccagcgt gctggaagcc atctatacgc tcggccatgg tcgggcgttt
    19561 cgcagtttgc agattggtcg cgtcattcgc cacgagcagg aggcgtttgt tctccacggg
    19621 cgattacagg gcgaagagcg cgagacagcg attggcttaa ccaaagacaa acagggcgac
    19681 agcaaagtcc gcatcgacgg tacagacggg cataaggtcg cggaactggc gcacctgatg
    19741 ccaatgcagt tgataacacc agaagggttt actttactca acggcggccc caaatacaga
    19801 agagcattcc tcgactgggg atgctttcac aacgaacctg gatttttcac cgcctggagc
    19861 aatctcaagc gattgctcaa gcagcgcaat gcggcgctgc gccaggtgac acgttacgaa
    19921 cagctacgcc cgtgggataa agaactgatc ccgctggcgg agcaaatcag cacctggcgc
    19981 gcggagtata gcgccggtat cgcggccgat atggctgata cctgtaagca atttctccct
    20041 gagttttctc tgactttctc tttccagcgc ggctgggaga aagagacaga gtacgctgag
    20101 gtgctggaac gtaattttga acgcgatcgc cagctaacct acaccgcgca tggcccgcac
    20161 aaagcggact tacgcattcg cgccgacggt gcgccggtgg aagatacctt atcgcgtggg
    20221 cagcttaagc tgttgatgtg cgccttacgt ctggcgcaag gagagttcct cacccgtgaa
    20281 agcgggcggc ggtgtctcta cctgatagat gattttgcct ctgagcttga tgatgagcgt
    20341 cgcgggctgc ttgccagccg cttaaaagcg acgcaatcac aggtctttgt cagcgcgatc
    20401 agtgctgaac acgttataga catgtcggac gaaaattcga agatgtttac cgtggaaaag
    20461 ggtaaaataa cggattaacc caagtataaa tgagcgagaa acgttgatgt cgaattctta
    20521 tgactcctcc agtatcaaag tcctgaaagg gctggatgcg gtgcgtaagc gcccgggtat
    20581 gtatatcggc gacacggatg acggcaccgg tctgcaccac atggtattcg aggtggtaga
    20641 taacgctatc gacgaagcgc tcgcgggtca ctgtaaagaa attatcgtca ccattcacgc
    20701 cgataactct gtctctgtac aggatgacgg gcgcggcatt ccgaccggta ttcacccgga
    20761 agagggcgta tcggcggcgg aagtgatcat gaccgttctg cacgcaggcg gtaaatttga
    20821 cgataactcc tataaagtgt ccggcggtct gcacggcgtt ggtgtttcgg tagtaaacgc
    20881 cctgtcgcaa aaactggagc tggttatcca gcgcgagggt aaaattcacc gtcagatcta
    20941 cgaacacggt gtaccgcagg ccccgctggc ggttaccggc gagactgaaa aaaccggcac
    21001 catggtgcgt ttctggccta gcctcgaaac cttcaccaat gtgaccgagt tcgaatatga
    21061 aattctggcg aaacgtctgc gtgagttgtc gttcctcaac tccggcgttt ccattcgtct
    21121 gcgcgacaag cgtgacggca aagaagacca cttccactat gaaggcggca tcaaagcgtt
    21181 cgttgaatat ctgaacaaga acaaaacgcc gatccacccg aatatcttct acttctccac
    21241 cgaaaaagat ggtatcggcg ttgaagtggc gttgcagtgg aacgatggct tccaggaaaa
    21301 catctactgc tttaccaaca acattccgca gcgtgacggc ggtactcacc tggcaggctt
    21361 ccgtgcggcg atgacccgta ccctgaacgc ctacatggac aaagaaggct acagcaaaaa
    21421 agccaaagtc agcgccaccg gtgacgatgc gcgtgaaggc ctgattgcgg tcgtttccgt
    21481 gaaagtgccg gacccgaaat tctcctccca gaccaaagac aaactggttt cttctgaggt
    21541 gaaatcggcg gttgaacagc agatgaacga actgctggcg gaatacctgc tggaaaaccc
    21601 aaccgacgcg aaaatcgtgg ttggcaaaat tatcgatgct gcccgtgccc gtgaagcggc
    21661 gcgtcgcgcg cgtgaaatga cccgccgtaa aggtgcgctc gacttagcgg gcctgccggg
    21721 caaactggca gactgccagg aacgcgatcc ggcgctttcc gaactgtacc ttgtggaagg
    21781 ggactccgcg ggcggctctg cgaagcaggg acgtaaccgc aagaaccagg cgattctgcc
    21841 gctgaagggt aaaatcctca acgtcgagaa agcgcgcttc gacaagatgc tctcttctca
    21901 ggaagtggcg acgcttatca ccgcgcttgg ttgtggtatc ggtcgtgacg agtacaaccc
    21961 ggacaaactg cgttatcaca gcatcatcat catgaccgat gcggacgtcg acggctcgca
    22021 cattcgtacg ctgctgttga ccttcttcta tcgtcagatg ccggaaatcg ttgaacgcgg
    22081 tcacgtctac atcgctcagc cgccgctgta caaagtgaag aaaggtaagc aggaacagta
    22141 cattaaagac gacgaagcga tggatcagta ccagatctct atcgcgttgg acggcgcaac
    22201 gctgcacacc aacgccagtg caccggcatt ggctggcgaa gcgttagaga aactggtatc
    22261 tgagtacaac gcgacgcaga aaatgatcaa tcgtatggag cgtcgttatc cgaaagcaat
    22321 gctgaaagag cttatctatc agccgacgtt gacggaagct gacctctctg atgagcagac
    22381 cgttacccgc tgggtgaacg cgctggtcag cgaactgaac gacaaagaac agcacggcag
    22441 ccagtggaag tttgatgtcc acaccaatgc cgaacaaaac ctgtttgaac cgattgttcg
    22501 cgtgcgtacc cacggtgtgg atactgacta tccgctggat cacgagttta tcaccggtgg
    22561 cgaatatcgt cgtatctgca cgctgggtga gaaactgcgt ggcttgctgg aagaggatgc
    22621 gtttatcgaa cgtggcgaac gtcgtcagcc ggtagccagc ttcgagcagg cgctggactg
    22681 gctggtgaaa gagtcccgtc gcggcctctc catccagcgt tataaaggtc tgggcgagat
    22741 gaacccggaa cagttgtggg aaaccaccat ggacccggaa agccgtcgta tgctgcgcgt
    22801 taccgttaag gatgcgattg ccgccgacca gctgttcact acgcttatgg gcgacgccgt
    22861 tgaaccgcgc cgtgcgttta ttgaagagaa cgctctgaaa gcggcgaata tcgatattta
    22921 atgacgttaa caatgcgagc gtgcctgatg cgctacgctt atcaggccta cgagactcgt
    22981 gcaatatatt gaatttgcat gattttgtag gccggatcag acgttaacgc cgcatccggc
    23041 aagaagctat aagaaaaggg cggagatcat cttcgccctt tttatttctg caatccgcaa
    23101 ttatcatccc gcagatctat actttttgag tcaacacgta acgctttaca ggtgattatc
    23161 atggggcttt ttgatgaagt tgtcggtgcc tttctgaaag gcgatgcggg gaaatatcag
    23221 gccattttga gctgggttga ggagcagggc ggcattcagg tgctgctgga aaaactgcaa
    23281 agtggcggtt taggtgccat tctctcaacc tggctgagta atcaacaggg caaccaacca
    23341 gttagcggcg agcaactgga atcagcgctc ggcacaaatg cggtgtccga tctcgggcaa
    23401 aaactgggcg tggataccag tacagcttcc agtttactgg cagaacaatt gccgaagatt
    23461 attgatgcac tctcaccgca aggtgaagtg acaacacaag ccaataacga tctgctttcc
    23521 gcaggcatgg aactgctgaa agggaaactc ttccgctaag caaaagggga gcatgcaacg
    23581 cgcgttgcat cccccttttc cctcgggtgt tgtactgatt tttgagcgga atcgcgttag
    23641 catgggtcag gaaccaatct acctggggaa ctcatggcta tcaaactcat tgctatcgat
    23701 atggatggca cccttctgct gcccgatcac accatttcac ctgccgttaa aaatgcgatt
    23761 gctgcagctc gcgcccgtgg cgtgaatgtc gtgctaacga cgggtcgccc ttatgcaggt
    23821 gttcacaatt acctgaaaga gctgcatatg gaacagccgg gcgactactg cattacttac
    23881 aacggtgcgc tggtgcagaa ggccgctgat ggtagcaccg tggcgcaaac cgctctcagc
    23941 tatgacgact accgtttcct ggaaaaactc tctcgcgaag tcggttctca tttccacgcc
    24001 ctggaccgca ccacgctgta tacagcaaat cgcgatatca gctactacac ggtgcatgaa
    24061 tccttcgttg ccaccattcc gctggtgttc tgcgaagcgg agaaaatgga ccccaatacc
    24121 cagttcctga aagtgatgat gattgatgaa cccgccatcc tcgaccaggc tatcgcgcgt
    24181 attccgcagg aagtgaaaga gaaatatacc gtgctgaaaa gtgcgccgta cttcctcgaa
    24241 atcctcgata aacgtgttaa caagggtacg ggtgtgaaat cactggccga cgtgttaggt
    24301 attaaaccgg aagaaatcat ggcgattggc gatcaggaaa acgacatcgc aatgattgaa
    24361 tatgcaggcg tcggtgtggc gatggataac gctattcctt cggtgaaaga agtggcgaac
    24421 tttgtcacca aatccaacct tgaagatggc gtggcatttg ctattgagaa gtatgtgctg
    24481 aattaatcag tgaacgggca aacggcatga atgcgtttgc ccgcagcgat taacaatttc
    24541 tggtcgtgca taaaatcagg aactggagtg gtgacgttac tatatccagc gtaagagtga
    24601 ctggcagcag cgcattaacg aggccgcgcg ggacgccatc tgttgctttg tactcataaa
    24661 atcccaccgt aaagggttga tggaaaacca tcacgtttga gtaatccgct tttttatttt
    24721 tctggtggat tgagcctgtc aaaccgtcca gcatccatgt acaacgccgg gtatcacaaa
    24781 taaaaccata ttgtgttgcc agtgccttat cttcttcgtt gttccagtcg taatagagcg
    24841 taatagcacc agagaaattt ttattactgc tgcttaaaat aaatttcgcg ttatcagaaa
    24901 cggtgattct ttcaccatga attgccggat cgttcagcat tttaaccacc ttatcgccgc
    24961 cggtggtcaa cagatagtcc aacgattcac caacaaaaac atagcctttc gtaccgttac
    25021 tatcttctgc cagcgataaa cccttaatcg tgtctgaccg ccattgctcg gcatgtggac
    25081 ggcttgcagt attggaaacc aatccccaag tgatacatcc ggtcaacgca aatgccaaag
    25141 aacacaaacc ggcagcctta aaaaatccct taaaattcaa cttcatgatt aatcctttaa
    25201 acgagtagct aagccgttga aagcatactc ttctttccct cctgtttagc gcctgacatc
    25261 ccttttcaga tttctgcccg acgcatgtca tttttttatg cattgttctt tttgtgatct
    25321 aaattgtagt acaacaatat aagtttgtac tacattacgc acacggcaaa cgcgaacgtc
    25381 atcacgctag tactacaaag ttgccgcgtt atgcatcgat cggggtaaag tagagaagaa
    25441 catacagagc acaaggactc tccatgactc tcaataaaac cgatcgcatt gtcattacgc
    25501 tgggtaaaca gatcgttcac ggcaaatacg taccaggctc gccgcttccg gctgaggcgg
    25561 agctctgtga
//
LOCUS       DAOTRK010000063        25470 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.063, whole genome shotgun sequence.
ACCESSION   DAOTRK010000063 DAOTRK010000000
VERSION     DAOTRK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.063"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..1251)
                     /gene="icd"
                     /locus_tag="QIE41_002454"
     CDS             complement(1..1251)
                     /gene="icd"
                     /locus_tag="QIE41_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDS3531332.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWMKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMDGAKLLKCSEFGDAIIKNM"
     gene            1833..2282
                     /locus_tag="QIE41_002455"
     CDS             1833..2282
                     /locus_tag="QIE41_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531333.1"
                     /translation="MDTTEERNGTYFYRGHPNLSKIELLNVIFLEEFCKELGIDSVSG
                     AAILSGQPWLGTRAKPQGATLGTSVVSKYARILMRDARMPFGISVHTPVGVRMQKSNK
                     LAAIIARWVPWIGWASLSMSLYRVSTRTQEKYNLIAKPQDRIKWTSF"
     gene            2267..2617
                     /locus_tag="QIE41_002456"
     CDS             2267..2617
                     /locus_tag="QIE41_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HDS3531334.1"
                     /translation="MDFLLMATTITDEVLALFRDEIPGYQDDHGKEIPLELDSDLFDY
                     PRDDLEDAIRKYKERFGINLDNLDWSLYLPWESAPLLQRWFKLKREEVETTRLPLTVR
                     MFAESAEAGKWLYQ"
     gene            2950..3588
                     /gene="rluE"
                     /locus_tag="QIE41_002457"
     CDS             2950..3588
                     /gene="rluE"
                     /locus_tag="QIE41_002457"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDS3531335.1"
                     /translation="MKYFSDSKSSFRKKQVSRLNSQHSTKRKPENQPTRVILFNKPYD
                     VLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGK
                     IYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGVELVDEPAWLWPRNPPIRERKSIPT
                     SWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            3598..4059
                     /gene="nudJ"
                     /locus_tag="QIE41_002458"
     CDS             3598..4059
                     /gene="nudJ"
                     /locus_tag="QIE41_002458"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDS3531336.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEANETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSRQRYPLEMIGDFNWPFTKGVI"
     gene            4113..5219
                     /gene="mnmA"
                     /locus_tag="QIE41_002459"
     CDS             4113..5219
                     /gene="mnmA"
                     /locus_tag="QIE41_002459"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDS3531337.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            5255..5896
                     /gene="hflD"
                     /locus_tag="QIE41_002460"
     CDS             5255..5896
                     /gene="hflD"
                     /locus_tag="QIE41_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDS3531338.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            5900..7270
                     /gene="purB"
                     /locus_tag="QIE41_002461"
     CDS             5900..7270
                     /gene="purB"
                     /locus_tag="QIE41_002461"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDS3531339.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            7439..8110
                     /gene="phoP"
                     /locus_tag="QIE41_002462"
     CDS             7439..8110
                     /gene="phoP"
                     /locus_tag="QIE41_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDS3531340.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            8110..9570
                     /gene="phoQ"
                     /locus_tag="QIE41_002463"
     CDS             8110..9570
                     /gene="phoQ"
                     /locus_tag="QIE41_002463"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDS3531341.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(10125..10406)
                     /locus_tag="QIE41_002464"
     CDS             complement(10125..10406)
                     /locus_tag="QIE41_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531342.1"
                     /translation="MTEAEILRLIRRVAGISQQADEQTTQPDSVTAENYVRVVAEVIR
                     RNGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            10572..11006
                     /locus_tag="QIE41_002465"
     CDS             10572..11006
                     /locus_tag="QIE41_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531343.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            complement(11282..11695)
                     /locus_tag="QIE41_002466"
     CDS             complement(11282..11695)
                     /locus_tag="QIE41_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDS3531344.1"
                     /translation="MATNESIMTLPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCISVDNAREILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            complement(11708..12043)
                     /locus_tag="QIE41_002467"
     CDS             complement(11708..12043)
                     /locus_tag="QIE41_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDS3531345.1"
                     /translation="MDTPYIELFAGSQQVATTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGKESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            complement(12055..13110)
                     /locus_tag="QIE41_002468"
     CDS             complement(12055..13110)
                     /locus_tag="QIE41_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDS3531346.1"
                     /translation="MTVKAMALNTNQLFAYLNRGDIAEFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQADGKKWSEQDKETHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQDAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            complement(13110..13316)
                     /locus_tag="QIE41_002469"
     CDS             complement(13110..13316)
                     /locus_tag="QIE41_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531347.1"
                     /translation="MAEEKKGGVSVYISPDIVKALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQVHGGE"
     gene            complement(13571..13819)
                     /locus_tag="QIE41_002470"
     CDS             complement(13571..13819)
                     /locus_tag="QIE41_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDS3531348.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETGKERHQVTMRRL
                     ECSRKAQRPPEPPTENYADLRNAVNRTYADMGLSKLAE"
     gene            complement(13830..14240)
                     /locus_tag="QIE41_002471"
     CDS             complement(13830..14240)
                     /locus_tag="QIE41_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDS3531349.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVMAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            complement(14237..14515)
                     /locus_tag="QIE41_002472"
     CDS             complement(14237..14515)
                     /locus_tag="QIE41_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531350.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKNVEVES"
     gene            complement(14804..16558)
                     /locus_tag="QIE41_002473"
     CDS             complement(14804..16558)
                     /locus_tag="QIE41_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDS3531351.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPDGMHGQKVENWREYLARERQNLSDGLVIELPVKQKAQLSQMADSERAQLLADRF
                     DGVCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLKHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAE
                     IPVIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKV
                     KGVTQTNAITTDECDAFL"
     gene            complement(16555..16854)
                     /locus_tag="QIE41_002474"
     CDS             complement(16555..16854)
                     /locus_tag="QIE41_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDS3531352.1"
                     /translation="MKLKYSGLTASGKTRTKFMRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFDRDFSLVQAVPRNVPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(16860..17087)
                     /locus_tag="QIE41_002475"
     CDS             complement(16860..17087)
                     /locus_tag="QIE41_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531353.1"
                     /translation="MHKIPFEVLIHSENALIKAREMNALLIKLIEVPEGGEDHSSLMF
                     AAVQTLLTPVIDELNKAMAIHENNKAPHTGE"
     gene            complement(17080..17310)
                     /locus_tag="QIE41_002476"
     CDS             complement(17080..17310)
                     /locus_tag="QIE41_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531354.1"
                     /translation="MHNKTTPDAAEEAMQILIRALVDVTHMVEIMERKSQSEHDKRKL
                     KTIKIIAKNSLIKVTDILNEDIKRIREKICDA"
     gene            complement(17300..17500)
                     /locus_tag="QIE41_002477"
     CDS             complement(17300..17500)
                     /locus_tag="QIE41_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDS3531355.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAAKIRQGETYAQ"
     gene            17694..17999
                     /locus_tag="QIE41_002478"
     CDS             17694..17999
                     /locus_tag="QIE41_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531356.1"
                     /translation="MATSNKNAKSQLTTVRVPLDVMQGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENPLVSSLDALAKVEQIGIKAAEEIGQLVTVAREELQRRKTKEPE"
     gene            complement(17894..18442)
                     /locus_tag="QIE41_002479"
                     /pseudo
     CDS             complement(17894..18442)
                     /locus_tag="QIE41_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="icd-like protein"
     gene            complement(18500..18688)
                     /locus_tag="QIE41_002480"
     CDS             complement(18500..18688)
                     /locus_tag="QIE41_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDS3531357.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(19053..20282)
                     /locus_tag="QIE41_002481"
     CDS             complement(19053..20282)
                     /locus_tag="QIE41_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDS3531358.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLNTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            20531..21652
                     /gene="roxA"
                     /locus_tag="QIE41_002482"
                     /gene_synonym="ycfD"
     CDS             20531..21652
                     /gene="roxA"
                     /locus_tag="QIE41_002482"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDS3531359.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(21701..22927)
                     /gene="pepT"
                     /locus_tag="QIE41_002483"
     CDS             complement(21701..22927)
                     /gene="pepT"
                     /locus_tag="QIE41_002483"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDS3531360.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            23177..24313
                     /gene="potA"
                     /locus_tag="QIE41_002484"
     CDS             23177..24313
                     /gene="potA"
                     /locus_tag="QIE41_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDS3531361.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            24297..25160
                     /gene="potB"
                     /locus_tag="QIE41_002485"
     CDS             24297..25160
                     /gene="potB"
                     /locus_tag="QIE41_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDS3531362.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(25392..>25470)
                     /locus_tag="QIE41_002486"
     CDS             complement(25392..>25470)
                     /locus_tag="QIE41_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDS3531363.1"
                     /translation="KYRVLLNRVDTSTAPDIEWPTSPAE"
BASE COUNT         6122 a         6479 c         6145 g         6724 t
ORIGIN      
        1 ttacatgttc ttgatgatcg catcaccaaa ctctgaacat ttcagcagtt tagcgccatc
       61 catcagacgc tcgaagtcat aggttacagt cttggcgttg attgcgcctt ccataccttt
      121 aacaatcagg tctgcggctt cagtccagcc catatggcgc agcatcatct cagcggagag
      181 aataatagag cctgggttta ctttgtcctg accggcatat ttcggcgcag taccgtgggt
      241 ggcttcaaac agggcgcatt cgtcaccgat gtttgcgcct ggggcgatac cgataccgcc
      301 gacctgtgca gccagagcgt cagaaatgta gtcaccgttc aggttcatac aggcgataac
      361 atcatattca gccggacgca gcaggatctg ttgcaggaat gcgtcagcaa tcacgtcttt
      421 aatgacgatc tctttgccgg tattcgggtt tttaactttc atccacgggc cgccgtcgat
      481 cagttcaccg ccaaactctt cacgcgccag ctggtagccc cagtctttaa acgcgccttc
      541 ggtgaacttc atgatgttgc ctttgtgcac cagggtcaca gagtcacgat cgttagcaat
      601 tgcgtattcg atcgctgcac gaaccagacg tttggtgcct tcttccgaac acggcttaat
      661 accgataccg caatgttccg ggaagcgaat tttcttcacg cccatctctt cacgcaggaa
      721 tttaatcact ttctcggcgt cggcagagtc agctttccat tcgatacccg cataaatgtc
      781 ttccgagttt tcacggaaga taaccatatc ggtcagttca ggatgtttaa ccgggcttgg
      841 ggtgccctga tagtaacgta ccggacgcag gcagatgtag agatccagtt cctggcgcag
      901 ggcaacgttc agagaacgaa taccgccacc aaccggagtg gtcaaagggc ctttaatggc
      961 aacgcgatat tcacgaatca gatcaagggt ttcagcaggc agccagacat cctgaccata
     1021 aacctgtgtg gatttttcac cggtgtaaat ttccatccag gagattttac gctcgccttt
     1081 ataggctttc tcgactgcag cgtcgaccac tttcagcatg gctggggtta catctacacc
     1141 gattccatca ccttcaatgt aagggataat cggattttca ggaacgttga gtttgccgtt
     1201 ttgcagggtg atcttcttgc cttgtgccgg aacaactact ttactttcca ttcacctctc
     1261 cttcgagcgc tactggtttg ctcgtctgcc accacgttgc atatgcgttt gcgtcctgcg
     1321 atacggatgc attagagcaa ttttttgtta atgatttgta attggcttgt caatactacc
     1381 ctattgtttg ccgtcatgaa agatgcgcgt tattaggcta taatgcggca attcataatc
     1441 tctgaaaata ccatgcaaaa aacttctttt agaccaaaga cagtattttt cagaccttca
     1501 aataaggcat taaatatcaa taagttacac cagtttttaa gcaataaaaa aaaccaccag
     1561 agataactaa caggatgcta tttggtcaaa atttgacatt actcgactca gcgactatct
     1621 cactttgagc actattggga aaattatcaa aagtcatggt tgaagcgaca agcaacgagt
     1681 aaattatggc atcccccctt tttgaggttc acatgaaacg ctttgcactg atagctgtga
     1741 aactcgttgc ctttactggt tctgcatctg ctgcaaccga agaaatcaac ttcaagtaac
     1801 agaaacacta tataacaatg gagtgttgac aaatggatac taccgaagaa cgcaatggaa
     1861 catatttcta cagaggacac ccgaacctgt ccaaaataga actactcaac gtcatattcc
     1921 ttgaagaatt ctgtaaggaa ttggggattg attcggtatc aggtgcagca atcttatctg
     1981 gtcaaccgtg gctgggtaca agagcgaagc cgcaaggggc aaccctggga acaagtgttg
     2041 tctcaaagta tgcgcgtata ctgatgagag atgcacgcat gccttttggt atttctgttc
     2101 atacaccagt gggtgtaagg atgcagaaat ctaataagct tgctgcaatt atcgctcgtt
     2161 gggtcccgtg gattggttgg gcttcactgt cgatgagtct ttacagggtt tcgactcgta
     2221 cccaggaaaa atacaatctg attgccaaac ctcaagaccg tattaagtgg acttccttct
     2281 aatggcgaca acaatcacag acgaagtttt agcactgttc cgtgatgaga ttccaggtta
     2341 tcaggatgac cacgggaagg aaattccgtt agaactggac tctgatttgt tcgactatcc
     2401 acgagatgat ttagaggacg ctatacggaa atacaaagag aggtttggga ttaacctgga
     2461 taatctggac tggtctttgt accttccatg ggagtctgca ccattgcttc aacgatggtt
     2521 caagctcaag cgggaagagg tagaaacaac acgtttaccg ttaactgtac ggatgtttgc
     2581 agaatcagca gaagcgggaa aatggctgta tcagtagaat aaaaaagaag gctgcgatgc
     2641 ccctcttatt actgctgatg atggtagtgg taaagcatca tcctgaactt gaacagtgaa
     2701 gtgctgtcac ccatcagtaa tacagaaaca ctatttttca taccagtatt tattcaattc
     2761 atcaatgtaa aatgtgttca ttgggccata tttatcacca tgcagccaca caaccgctgc
     2821 tcactaaccc aggtattgat gaaaccgcgc tgaagagcct gaaccctgaa cctctaacga
     2881 gtaccttaaa agtccaatgc aatccgacac tgtgctaccc tctgcgtata gctctatttg
     2941 gaatttacca tgaaatactt tagcgattct aaatcttctt ttagaaaaaa acaggttagt
     3001 cgactaaact cgcaacactc cactaagcgt aaacctgaaa accagcccac gcgcgtgatc
     3061 ctgttcaata aaccctacga tgttcttccg cagttcaccg atgaagccgg acgcaaaaca
     3121 ttaaaagaat tcatcccggt tcagggcgtt tatgcagcag gtcgtcttga ccgcgatagc
     3181 gaagggttac tggtgctgac caataacggc gcgttgcagg cgcgtttaac ccagccgggt
     3241 aaacgcaccg gaaaaatcta ttatgtgcag gtggaaggta ttcccacaca agacgcactt
     3301 gaagccttgc gcaatggcgt aaccttaaat gatggcccta ccctgcccgc aggcgtagag
     3361 ctggttgacg aacccgcgtg gttatggccg cggaatccac caattcgtga acgcaaaagt
     3421 attcccacca gctggctgaa gatcacctta tatgaaggac gtaatcgcca ggtgcgccgc
     3481 atgaccgccc atgttggttt ccccacgctg cgactgattc gctatgcgat gggtgattac
     3541 tctttggata atcttgccaa cggcgaatgg cgagaagcga cagattaagg acgcaaaatg
     3601 tttaaaccgc acgttaccgt tgcttgcgtg gtgcacgcag aaggcaaatt tttagtcgtt
     3661 gaagagacga ttaatggtaa agcgttatgg aaccaacctg ccgggcatct ggaagccaat
     3721 gaaaccttag tggaagccgc cgcccgtgag ctgtgggaag aaaccggcat cagcgcgcag
     3781 ccgcaacact ttattcgtat gcatcagtgg attgcgccag ataaaacgcc gtttttgcgt
     3841 ttcctgtttg ctattgagct tgagcaaata tgcccgaccc agcctcatga cagcgatatc
     3901 gactgctgcc gttgggttag cgccgaagaa attttacagg cgtcaaatct gcgttcgccg
     3961 ctggtggcgg aaagtattcg ttgttatcaa agcaggcaac gctatccgct agagatgatt
     4021 ggcgatttta actggccttt tacaaagggt gtcatctaaa aggctgcgtg atagaatacg
     4081 ccgccttgaa gttcaatgtc gtgagtgatc caatgtctga aaccgcaaaa aaagtaatcg
     4141 tcggcatgtc cggcggtgtc gattcctccg tttctgcctg gctgttgcaa caacagggat
     4201 atcaggtcga aggcctgttt atgaagaact gggaagaaga cgacggtgag gaatattgca
     4261 cagcggcagc ggatctggct gatgcccagg ctgtctgcga caagctcggc attgaactgc
     4321 acaccgttaa ctttgctgcc gagtactggg acaacgtctt cgaactgttc cttgccgaat
     4381 ataaagccgg tcgcacgccg aatccggata ttctgtgcaa caaagagatc aaatttaaag
     4441 ccttcctcga atttgccgct gaagatttag gtgccgatta tatcgctacc ggtcattacg
     4501 tacgtcgggc agatgtcgat ggcaagagcc gcctgctgcg tggtctggac agcaataaag
     4561 accagagcta cttcctttat acgctcagcc atgagcagat tgcgcaaagc ctgttcccgg
     4621 tcggcgaact ggaaaaaccg caggtgcgta agattgctga agatctcggt ctggtcaccg
     4681 cgaagaaaaa agactctacc ggcatttgct tcattggcga gcgtaaattc cgcgagttcc
     4741 tgggccgtta tctcccggcg caaccgggca aaatcattac cgtcgatggc gatgaaattg
     4801 gtgagcacca ggggctgatg tatcacaccc tcggtcagcg taaaggtctg ggtatcggtg
     4861 gcaccaaaga cggcaccgaa gaaccgtggt atgtggtcga taaagacgtc gaaaacaaca
     4921 ttctgattgt tgcgcagggc catgaacacc cgcgtctgat gtctgtcggt ctgattgccc
     4981 agcagctgca ctgggtcgat cgcgagccgt tcaccggtac tatgcgttgc acagtaaaaa
     5041 cccgctatcg ccagaccgac atcccgtgca ccgtcaaggc gctggacgat gatcgcattg
     5101 aagtgatttt cgatgagccg gttgccgccg tgacgccggg ccagtctgcc gttttctata
     5161 acggtgaagt gtgcctcggt ggcggtatta ttgagcagcg tctgccgctg ccggtctgat
     5221 tattatcttt acttaaaaca aggaagcagt gaacgtggca aagaattact atgacatcac
     5281 cctcgcccta gccggtattt gtcagtcggc acgcctggtg caacaactcg ctcaccaggg
     5341 gcattgtgat gccgatgcgc tacacgtctc actcaacagt attattgata tgaaccccag
     5401 ctcgacgctg gcggtttttg gcggcagcga agccaacctg cgcgtcgggc tggaaacctt
     5461 gctcggcgtg ctcaatgcca gcagtcgcca gggcttaaac gctgagttaa cccgctacac
     5521 actcagcctg atggtgcttg agcgcaaact ctcctcagcg aaaggtgcgc tcgacactct
     5581 gggcaaccgg atcaacggcc tgcaacgcca gctcgaacac ttcgatttac agtccgaaac
     5641 gctgatgagc gcgatggctg ctatctatgt tgatgtgatt agcccgcttg gcccgcgcat
     5701 tcaggtaacc ggttcccctg ctgtactgca aagcccacaa gtgcaggcga aagttcgcgc
     5761 aaccctgctg gcaggcattc gcgccgccgt gctctggcac caggttggcg gcggacgtct
     5821 gcaactgatg ttttctcgta atcgcctgac cactcaggca aaacaaattc ttgctcattt
     5881 aaccccggag ttgtgatcta tggaattatc ctcactgacc gccgtttccc ctgtcgatgg
     5941 acgctacggc gataaagtca gcgcgctgcg cgggattttc agcgaatatg gtttgctgaa
     6001 attccgtgta caagttgaag tacgttggct gcaaaaactg gccgcgcacg cagcgatcaa
     6061 ggaagttcct gcttttgctg ccgacgcaat cggttacctt gatgcaattg tcgccagttt
     6121 cagcgaagaa gatgccgcac gcatcaaaac catcgagcgt accactaacc acgacgttaa
     6181 agcggttgag tatttcctga aagaaaaagt ggcggagatc ccggaactgc acgcggtttc
     6241 tgaattcatc cactttgcct gtacttcgga agatatcaat aacctctccc acgcattaat
     6301 gctgaaaacc gcgcgtgatg aagtgatcct gccgtactgg cgtcaactga ttgacggcat
     6361 taaagatctc gccgttcagt atcgtgatat cccgctgctg tctcgtaccc acggtcagcc
     6421 agccacgccg tcaaccatcg gtaaagagat ggcaaacgtc gcctaccgta tggagcgcca
     6481 gtaccgccag cttaatcagg tggaaattct cggcaaaatc aacggcgcgg tcggtaacta
     6541 taacgcccac atcgccgctt acccggaagt tgactggcat cagttcagcg aagagttcgt
     6601 cacctcgctg ggtattcagt ggaacccgta cactacccag atcgaaccgc acgactacat
     6661 cgccgagctg tttgattgcg ttgcgcgctt caacaccatt ctgatcgact ttgatcgtga
     6721 cgtctggggt tatatcgccc ttaaccactt caaacagaaa actattgctg gtgagattgg
     6781 ttcttccacc atgccgcata aagttaaccc gatcgacttc gaaaactccg aagggaacct
     6841 gggcctttcc aacgcggtgt tgcagcacct ggcaagcaaa ctgccagttt cccgctggca
     6901 gcgtgacctg accgactcca ccgtgctgcg taacctcggc gtgggtatcg gttatgcgct
     6961 gattgcgtat caatccacct tgaaaggcgt gagcaaactg gaagtgaacc gtgaccatct
     7021 gctggatgaa ctggatcaca actgggaagt gctggctgag ccaatccaga cagttatgcg
     7081 tcgctatggc atcgaaaaac cgtacgagaa gctgaaagag ctgactcgcg gtaagcgcgt
     7141 tgacgccgaa ggcatgaagc agtttatcga cggtctggcg ttgccagaag aagagaaagc
     7201 ccgcctgaaa gcaatgacgc cggctaacta cattggtcgc gccatcacca tggttgatga
     7261 gctgaaataa gcctcaaaac agtgccggat ggcgatgctg tccggcctgc ttattaagat
     7321 tatccacttt ttcttttttc actttacctc ccctccccgc tggtttattt aatgtttacc
     7381 cccataacca cataatcgcg ttacactatt ttaataatta agacagggag aaataaaaat
     7441 gcgcgtactg gttgttgaag acaatgcgtt gttacgtcac caccttaaag ttcagattca
     7501 ggatgctggt catcaggtcg atgacgcaga agatgccaaa gaagccgatt attatctcaa
     7561 tgaacattta ccggatattg cgattgtcga tctgggattg ccagacgagg acggtctgtc
     7621 actgattcgc cgctggcgta gcaatgatgt ttcactgccg attctggtat taaccgcccg
     7681 tgaaagctgg caggacaaag tcgaagtatt aagtgctggc gctgatgatt acgtgactaa
     7741 accatttcat attgaagagg tgatggcgcg aatgcaggca ttaatgcggc gtaatagcgg
     7801 tctggcttca caggtcattt cgctaccccc gtttcaggtt gatctctctc gccgtgaatt
     7861 atctattaat gacgaagtga tcaaactgac cgcgttcgaa tacaccatta tggaaacgtt
     7921 gatacgcaat aatggcaaag tggtcagcaa agattcgtta atgctacaac tctacccgga
     7981 tgcggagctg cgggaaagtc ataccataga tgtactgatg ggacgtctgc gcaaaaaaat
     8041 acaggcacaa tatccgcaag aagtgattac caccgttcgc ggccagggct atctgttcga
     8101 attgcgctga tgaaaaaatt actgcgtctt tttttcccgc tctcgctgcg ggtacgtttt
     8161 ctgttagcaa cggcagcagt agtattggtg ctttcgcttg cctacggaat ggttgcgctg
     8221 atcggttata gcgtcagttt cgataaaacc acgtttcggc tgttacgtgg cgagagcaat
     8281 ctgttctata cccttgcgaa gtgggaaaac aataagttgc atgtcgagtt acccgaaaat
     8341 atcgacaagc aaagccccac catgacgcta atttatgatg agaacgggca gcttttatgg
     8401 gcgcaacgtg acgtgccctg gctgatgaag atgatccagc ctgactggct gaaatcgaat
     8461 ggttttcatg aaattgaagc ggatgttaac gataccagcc tcttgctgag tggagatcat
     8521 tcgatacagc aacagttgca ggaagtgcgg gaagatgatg acgacgcgga gatgacccac
     8581 tcggtggcgg taaacgtcta cccggcaaca tcgcggatgc caaagttgac cattgtggtg
     8641 gtggatacca ttccggtgga gctaaaaagt tcctatatgg tctggagctg gtttatctat
     8701 gtgctctcag ccaatctgct gttagtgatc ccgctgctgt gggtcgccgc ctggtggagt
     8761 ttacgcccca tcgaagccct ggcaaaagaa gtccgcgaac tggaagaaca taaccgcgaa
     8821 ttgctcaatc cagccacaac gcgagaactg accagtctgg tacgaaacct gaaccgattg
     8881 ttaaaaagtg aacgcgaacg ttacgacaaa tatcgtacga cgctcaccga cctgacccat
     8941 agtctgaaaa cgccactggc ggtgctgcaa agtacgctgc gttctctgcg tagtgagaag
     9001 atgagcgtca gtgatgctga accggtaatg ctggagcaaa tcagccgcat ttcacagcaa
     9061 attggctact acctgcatcg tgccagtatg cgcggtggga cattgctcag ccgcgagctg
     9121 catccggtcg ccccactgct ggacaatctc acctcggcgc tgaacaaagt gtatcaacgc
     9181 aaaggggtca atatctctct cgatatttcg ccagagatca gctttgtcgg tgagcagaac
     9241 gattttgtcg aggtgatggg caacgtgctg gataatgcct gtaaatattg cctcgagttt
     9301 gtcgaaatat ctgcaaggca aacagacgag catctctata ttgtggtcga ggatgatggc
     9361 cccggtattc cattaagcaa gcgagaggtc attttcgacc gtggtcaacg ggttgatact
     9421 ttacgccctg ggcaaggtgt agggctggcg gtagcccgcg aaatcaccga gcaatatgag
     9481 ggtaaaatcg tcgccggaga gagcatgctg ggcggtgcgc ggatggaggt gatttttggt
     9541 cgccagcatt ctgcgccgaa agatgaataa atatgtcctt ttatcactac atcaaggcaa
     9601 gccattgatt taattaaatg atttgcactg caaaaagtgc taaaaatcgc aaattgacta
     9661 caccattaac tacaccgatc gttgcactgt atgaaacaac gtggaacaaa tagacacaag
     9721 aaatatacag gcgggtcatc tttccagggg gaagcgcgcc aattcatgag gggcgttaat
     9781 gtcgatatgg ggatccccat aacggggcta cctggctttt ttccggttaa atgtcaatca
     9841 ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt gcgcctttcc
     9901 accaatggtg gaaaagccca cagcaggccg cccagcctcc cccaatgggg gattgtggaa
     9961 gaatcaacgg gttaggcctt aatgggctgc gtggtaatta tcagcatcca gaatatgacg
    10021 ggttatgttt ccgacctctt caatttgagg ttcccgaaaa tatcaacggg ttaggctggt
    10081 ttccccgatg ttcgccgctg gcgaatttcg aaaatatcaa ccagttaccg ccgcaaccgc
    10141 tccggcttct tccagtggta cgtaattttc tccgtttccc gatacagtgc cacgcggcga
    10201 ttgtaggcca gcatttcaag aacgcggatc cgtatgttgc gcatatccac atcattaagc
    10261 tggataccat tacggcgaat cacctcagca accacacgaa cataattttc ggcggtcacg
    10321 ctgtccggct gcgtggtctg ttcgtcggcc tgctggctga ttccggcaac gcggcggatt
    10381 aatcgcagta tttcggcttc tgtcattgtg cccccatcgt tctgatagtc tggtgtcgtc
    10441 gggtccttcc tggaattatg gcccgttacg gggcggcgac ctcgcgggtt ttcgctattt
    10501 atgacgtttt tccgtgaagg tgacaccacc accacttgat taatatttaa ccatacagtt
    10561 aaggtaacat tatgattgat aaagcttgtt ttgtaagtca gcaggaaata gctgaacatt
    10621 tcaaggttaa cagaaccact attcgcgcat ggaccaaaca ggggatgccg tatcttaatg
    10681 cggatcgcgg aaagtctggc ggttatcaca tcgggcatac attgctttgg tcttcaggta
    10741 aaagccgtct tgaggccatc agatatcacg tagaaaccag tgcgctggaa aaaattatgt
    10801 ttgccaggct gctttcatct gagcgtgacg agtactccag tgaagaaaca gaacatcgat
    10861 ttgatgaagg tttgcagatt tacggctatt caccggaaga tgtgagcaag gcacgaaata
    10921 aaatggctgg ctttctggct gggtggcgtc atgccgtaag cgttcgccgt gccagcatgg
    10981 agcaatcagc cgatacagaa cagtaaagcc gataacttgt taatcactcg cagcaatgca
    11041 tgtgatttgt gcacactttc ggttaactgg ttcgatgtcc ggttttagtg tctgtttttt
    11101 gcgcatgtcc ggttcatgga aagcatgttt ttatattttt catatggtta acttgcagag
    11161 aaaccggaca tggatcccga aaaattttca taaatagtga aaacgcgcga ggtcgccgcc
    11221 ccgtaacggg ccataattcc aggaaggacc cgtaaaaaag ccggatttct ccggccttgt
    11281 ctcagatggt tttcagtatg cgatcgatgt cgccgtcatc gccctggttt ctgccatcgt
    11341 atgccatgcc agctgatacg gcttgcgggc tgtgcatgtc cataaagttt tcaaaggctg
    11401 cggtaagctc tggtgcaacc tttgggcgct cctgctctat ggtcatgttc aggatttcac
    11461 gagcattatc gacgctaata catggcacgt ttgccattgc acgtaacagc ggctgatagt
    11521 ctttatgctc atgaagcgcc ataatcgcat cagcgcgcgg cttgtcctgc tcttccagtt
    11581 tgtttagttg atatacggcc tcataggttg ataaacctct gtcagccatt gcccgcgctt
    11641 cggctttaaa tttactcgcc agcggtagcg tcatgatgct ttcattcgtt gccatcgttc
    11701 cccctgctta tcgggccagc ggctgaacgg atacgccgga acccgcaaag gcggcgcatt
    11761 ttttcgcatc ggtgtcgacg ctctcaggcc agttaacggc ggcaatattg aatatccccg
    11821 tcttgtaaca ctgtgctgat ttctgctttg acgtgtccac agggtacgag gtcagataaa
    11881 cagccttgcc agattcctta ccatcccacg gcttaaactc gccattgtcc gccagcatca
    11941 gcggggtaaa ttcctgaata acgccagcat cagcggcaaa atgtaccagc gtcgtggcga
    12001 cctgctgact gcctgcaaat aactcaatgt atggagtgtc catagaatcc cccgttaacc
    12061 aattttgacg gtaacaaatt tgcgaatatc tgccggaacc ggctgcggtg cgctgtgcgt
    12121 ctgcacgtac tcaatcgccg gatcgccgtc ctcaatccag tttttcgggt aaaacatgtt
    12181 ttgcgttgcg cccgttctta ctgcgtcctg atccataatc gcaccatagg ccaccagccc
    12241 tttattgttg gtgttgccca ggacaagcaa atcaggctca aggaaatatt tttctgtgcc
    12301 gtcgctgtca gtgtatttgc cggaatagac gataagggca atatcgccca gatagccttt
    12361 aaagctcacc acttcgccca ggtttttaca ggccagctct gcggcggact ctgaaccacg
    12421 ggaaagatcg tacagctcac ggaatttttt aaagctgcgt aacgttcgcc atacctcagc
    12481 gcccataatc atgacgtttg cggggcaacc agcctgatcg gcgtatagtt cgatatcata
    12541 gattggatcg tgggtttctt tgtcctgctc tgaccatttt ttcccgtcgg cctgctctat
    12601 gatgtttttt tccggtatct tccagtcgat ttcatagcgc tctatgcctt cgccttcaat
    12661 gatgtttttt ccggtcgtta ccgcatttac cgccagccat tccacgcgcg cttttatggc
    12721 gtttatctgg cggcgcatat tgccagtaat caggcgcata cggcgatagg tagggtcgtt
    12781 aagctgtgcc ggatcttctc cagccatgcg catgatggtt ttcgttggat cgatttcgtg
    12841 ctttggcttc atgtagccag gtttaatcgt gctggtttcg taccctttat cgcgctgaac
    12901 ctggctaccc accataggcg aacaaaacgc cgacatggtg acttcttcaa tgtccagggt
    12961 atccagcatg atgttttgcg tgctgaatgt cgccacgttc gggaaaaaca gcgtggtaaa
    13021 cagcggactg aatttaaatt ccgcaatatc cccgcgattc aggtacgcaa aaagctggtt
    13081 agtgttaagt gccattgctt ttactgtcat tattcacccc cgtgaacctg attcataccc
    13141 agcgccgcgc gtaaataggc gcgtacctgc cagcctgttg acggctcaac catcgccagc
    13201 ggatcaagtc ctgccgcaat gcctgctttt acgttctgct ggtggcgttc cttgagcgcc
    13261 ttcacgatgt cggggcttat gtacaccgaa acaccgcctt ttttctcttc agccatagta
    13321 agaaattcct ctttgactta aaaaatcata actggatgtt catccagtta tgattataat
    13381 catgattgca ttttgtgcaa tgatattgag ttgcattgca aattatgaaa tgattatccc
    13441 gatcatgtgt gtcagtgcac caaaaaacct catatgcaaa agcccgataa gccacctctg
    13501 accttatcgg gctttttttg ggcgcaaaaa agccggattg ctccggctgt gtggtgtggt
    13561 tctggtattc ctactctgct aatttgctca gccccatgtc tgcgtaagtg cgatttactg
    13621 catttctcag gtctgcatag ttctccgttg gcggctccgg tggcctctgt gccttcctgg
    13681 aacattccag ccgtcgcatc gtaacctgat gccgttcctt gcctgtctcc accagttgca
    13741 tgacttcacc ccatcgtgcc gccgccctcc ggtaaaagcc ttttgcctcg agttcctccg
    13801 ctatgcggtc atgtaccatc cttaccccct cagaacggaa tatcatcacc gtaagggtca
    13861 tcgcctcccg ctggtggctg attaccctgt gtgcctgtgg ttttgcgtct gttcccgcca
    13921 ggacgtgccg cacgggcact gattacgctg tctgcaataa cctgataacc ctgccgcgtt
    13981 tccccgttct gtccggtcca ctggctgacc tgcatcgtgc cggatacgct ggcaacgtcg
    14041 cctttttgat gtttagccag gaagtcggcc tgcttaccaa atgccatcac cgatagccat
    14101 aacgtagcct gcccatcctg cgcctgacta catggcaacg ataccgccat acgtgccagc
    14161 gtcattggtg tgcccttgct ggtctgtttt acctgcgggt cgtccaccag ccgcccgtaa
    14221 gccgctatct gtgctgtcat gattccacct ctacgttttt aacgttgatg gttgtcacct
    14281 gttcagcttc cgcaatctcc cgctctgtca gcgtggcaaa atttgccgcc gtcgtggtca
    14341 tgaatgcgct tatcagttcg ggatgtgctt tcgcgtatcc ttcccccgcg tggcggtcta
    14401 tcgttctgat tgccaccttt aaagcgtgct ctgtcatgtc taacgcgcga tatttcggtt
    14461 ctgttctgtc tctgcgtttt ttgagtgatt tattaaattt ccctgaagtg tgcatattta
    14521 tttttacccc ctcgtttaaa aagttttgag ttgtgcctcc ccttgtctac cttatctacc
    14581 ttagtggccc tcatgccagt aatggcacgg ctttcagcgg ggtagagtgc ttttatccac
    14641 tatctacccc gtgtctacct ccctgtctga ttcaggtaaa atcaggtaga gagggtagat
    14701 agtgggtaga cagtaaaaaa aggctgtcta cctaacttaa tgcactgaat taaatgtatt
    14761 tttctttact caggtagaca gggtagacag caattacaaa aaattataaa aacgcgtcgc
    14821 actcgtctgt tgttattgcg ttagtctgcg ttaccccctt aactttccgc gtaatatatt
    14881 catgtccgta aacttttgcg gcaggcttca tagccttgcc aaagtcattt acgtttagcg
    14941 gtttgctcct gcctgcgtac gccataaacg ccagatagac gcggtaaagg ctgtttctgg
    15001 tcgtgtactt cactgaatcg ccaccgccgc ccatcatcag gccgcgtgct tcctccagaa
    15061 aattcaggaa ctggcaaaac tcaataaccg gatccgtctg ttgctttatt gccagtgctt
    15121 catcaccgtc acgctgttcc agtagtaaag cccgtgcttt ctcagagtcg gtaaagttcg
    15181 ccagcaatcg gcggataatg acagggattt cagccgcaat cttttccggt agctccctgt
    15241 ctttttcggc ctcactgacg atattgtcga aacggaaaat cacgcgacga cgtgccacac
    15301 ctccggcccg ttcggtgaat atcatcgggt tgttgttggt cgccagcacc accgccctga
    15361 ttaccgccgt aaaacgcttt tcatatttcg ggttaatttc cacggggtca ccgcccgtga
    15421 ttttcttgat gcccgttcct tcgcctgtat atttcggctg gtcagccagg acgataagac
    15481 gactcccgac aacctgcgca cgtccaccag catcatcaag cgatgtcatt tcagcgctta
    15541 ccgtgttctg tttccctgcc agaaggctgg ctatgtgcgt gaatgtactt ttaccgctcc
    15601 cgccgtctcc ggtggcctca ataaacatct gccagtcgta ccggttcgcc ataatcatgt
    15661 acagcgcggc acatatacgc atcatcttgc gcgggtcttt tccggctgcg tgcttaagcc
    15721 atttatgaaa gtttggcgcg ttatcgcgga tgttctcccc tggtgctggt ggcgtgtact
    15781 caatgccgtt gtgcgtggtg atccagttct ccggcgtgtg cggggaaaat tcccccgttt
    15841 tcaggtcaag cgcaccattg gcgaacggca gcaaatcgcc ggacggctcg cccattggtt
    15901 cggcaataac ttttaacgct tccacggcgt tattgattac gcgcttgctg aaagtggccc
    15961 tgtgctctga atagatcgcc accatttcgc ggctaagttc cattgtgctg accggacacc
    16021 ataccccgcc gcgccatacg tgaacgattt cactttcagg atgtacgcaa acgccatcaa
    16081 agcgatcggc aagcagctgc gcgcgctcac tgtccgccat ctgcgaaagt tgcgcctttt
    16141 gctttaccgg aagctcaatg accagaccat cagaaagatt ctggcgctca cgggccagat
    16201 attcgcgcca gttctccacc ttctgaccgt gcataccatc agggtaaaaa tttgcgtcct
    16261 gaatatctgc cgccgccagc ttctgaccaa tctttttggt ctccactaaa tccagttctc
    16321 cggcctggta cagccttacg cgctttttcc catccggaac aattttcagc gcatcaagtt
    16381 cggcaagctg atttggccca agccacacag gcggcacatt atcgccggat gcggggccgt
    16441 cctgctcctg ccactgtttt gcatgtgacc acgcatcact acccgcgaaa ataattacct
    16501 ctgtgtcttt gtgttttatt ccgcgtggtt gtttttttac gttcggtgcc agtttcattt
    16561 cttacccctg aatacgttaa gcatcttttt tatttcctga atattggcgc gtgctttctc
    16621 cctgctggtg ggcacgttac gcggtacggc ctgcaccaga gaaaaatcac gatcgaactg
    16681 ataaacaggc atcacgcaat catattcgta gccttcacga cggtaggtta cgcgccgttc
    16741 ctccacgccc ttaatcatta ccgtgccgcc gtactggtcg cggtaaatat caccgcgcat
    16801 gaatttagtg cgagttttgc cactggcagt taagccagaa tatttaagtt tcattatttt
    16861 tattctccgg tgtggggcgc tttattattc tcgtgaattg ccatagcctt attgagttca
    16921 tcaataacag gtgtcaataa tgtctgaaca gcggcaaaca ttaatgatga atgatcttcg
    16981 ccaccttccg gcacttcaat taatttaatt aacaacgcat tcatttcgcg tgctttaatt
    17041 aatgcgtttt cagagtggat taatacttca aaagggattt tatgcatcac aaattttctc
    17101 ccttattctt ttaatgtcct cattaaggat gtctgtaact tttatcagcg agtttttggc
    17161 aattattttt attgttttaa gttttctttt gtcgtgctcc gactgtgatt ttctctccat
    17221 aatctccacc atgtgggtaa catcaacgag cgcacgtatc agtatttgca ttgcttcttc
    17281 tgctgcgtcc ggtgtggttt tattgtgcat aggtttctcc ctggcggatt tttgcagcga
    17341 atacagcaac acaaccggac gggcaacggc tacgcgcttc gcgttccgtc caggcggtta
    17401 cgtggatgat ttgagattct ccggcactca gggccagaaa acgccacaca aaggccgttt
    17461 gtgtgtgtac aaggtgtggt atatgattta cagcaaccat aaatttttcc tctctatagt
    17521 gtttgtggtt agacgccccg tatgtgtttc cggcactgcg gggcgttgct ctttgtattt
    17581 caacaatcct ttcggtgtgt ttcatgttat gagcgcatga aacacacgtc aaggcttttt
    17641 gtatttcttt ttttgtgtat actgaaacac accgatgatt aggagtttca gaaatggcaa
    17701 cgtctaacaa aaacgcaaaa tcacaactga caactgtcag agtcccacta gatgttatgc
    17761 aagggatgga atccgttaag ctggacggcg aaagcaatgc cggatttatc gtaaccgcca
    17821 tgcgcggaga aatagcccgc cgccaagcag aaggcagcgg agaaaatccc cttgtgtcgt
    17881 cactggatgc cctggctaag gtcgaacaaa tcggcatcaa agcagcggaa gaaatcgggc
    17941 agcttgtcac cgttgcacgc gaagaactcc agcgtcgcaa gaccaaagaa ccagagtaat
    18001 caccatcagc gccgtggtgt aaggtattac ggcgcattgc tatgcaggac aacacaatga
    18061 ccgataaaga attgaccaaa acattatcac cggcacggaa aagacggcgc agaaagatag
    18121 aacatgaatc agaaagattc gcgccatgtg cttttgccct tgagcaattc cttaaagagt
    18181 acagggaaaa gcgctcattg caggtatggc aacgaactga accagactga ttgcattgcc
    18241 caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac acacaaacgg
    18301 cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt tttatgcctg
    18361 aaaaacccca attttgtggt tttccagtct caccagggcg aacgaatccc cgcccacgtt
    18421 ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga tgtgtggcgg
    18481 ctgtgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag gccagaacct
    18541 cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg aattgtccgg
    18601 ccttttccag gcggtagcga cacgtacggc taaggccggt tagtttttcg cattcttttt
    18661 cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta aagagtcatc
    18721 aggtgtctta ttgtgtcgta ttgttcccgc tagagtgatg aatggcaaat actgaggatg
    18781 tatgatttac agaaaatgaa acagtaagaa taaaatcttt taaattcatg ttaatacaaa
    18841 ggaataaaat attgttccat gccttttttc tcgctcttta aagagtgatt cgctagtgta
    18901 taaaaaacca gtaacacatt aaaaatcagc cacttataaa tccgtacgct ttttcgcctc
    18961 ttgttcgtag ttgttccaca ttgttgctca ttgttgcacg ttgtatctgt tcgcatatcc
    19021 agtatgtgca tactgaaaaa acacgaaaaa aattattttc ttctggctac tggtagcgtg
    19081 gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat atccagcgca
    19141 ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc tggcatctgg
    19201 tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag catcgttgta
    19261 aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct gtgcgctaat
    19321 ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa gcccgtgtgc
    19381 ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat gggccggaat
    19441 attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc ggtaaaatcc
    19501 cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac aatcaggagg
    19561 cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg caataattcg
    19621 cccagttctt tggtgtttaa gacacgctcg cttatatccg gttttttccc aacgtccgcc
    19681 acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca gaacttaagc
    19741 gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt tgccacctgg
    19801 tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc accaatgtgc
    19861 tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg gttttccttt
    19921 gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt taacgcttcc
    19981 tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca tgcgcgacac
    20041 tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc cagcttaatg
    20101 cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca ggttaaaagg
    20161 ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc tgttttttgg
    20221 gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag tgctctgctc
    20281 ataattttga ttccggtcat cacaattaac tacaccatta actacaccga tcgttgcaca
    20341 aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt tataatcaaa
    20401 aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg ataaaatatg
    20461 tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata ccagcctgtg
    20521 gatgcttaac atggaatacc aactcactct taactggccc gattttcttg aacgtcactg
    20581 gcagaaacgt ccggtggtgt taaaacgcgg ctttaataat tttattgacc cgatctctcc
    20641 agacgagttg gcgggtttgg cgatggaaag cgaagtcgac agtcgactgg tcagtcacca
    20701 ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc tcggtgaaac
    20761 taactggtca ttgttagtgc aggcagtgaa ccactggcat gagccgaccg ccgcgctgat
    20821 gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgatct ctttttctgt
    20881 acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca ttcagggtac
    20941 cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact gcccacatcc
    21001 ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg agcctggcga
    21061 tatcctttat attccgccag gattcccgca tgaaggctac gcgctggaaa atgcgatgaa
    21121 ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat ttgccgatta
    21181 tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc cacctcgcgc
    21241 tcatcctgcg gacgttctgc cgcaagagat ggataaactg cgtgagatga tgctcgaatt
    21301 gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc agtcacgtca
    21361 tgaactggat atcgcgccgc cagagccgcc ttatcagccg gatgaaatct acgatgcgct
    21421 aaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga
    21481 cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg atgcgctcgc
    21541 cagcaacatt gcgctgaccg cggagaattt tggtgatgca ctggaagatc cgtcattcct
    21601 cgcgatgctc gcagcgctgg tcaatagcgg gtattggttc ttcgaagggt aagtttgaag
    21661 ttaagctgga tgcggcatca catgccgcat cccttttcgc ttacttccgt tgcgccgtca
    21721 actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga gtcacaaact
    21781 catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcacggt aatcccataa
    21841 acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt tcaatatcgc
    21901 agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc actttctcgc
    21961 gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga tgtaaccctt
    22021 tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac tgtttacggt
    22081 cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc atgctcgcca
    22141 ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg
    22201 catgaatacg tgccgccagc gacagcgcat tcaccattac acctttcgct gtgcctgggt
    22261 gaacgttatt gcctacaatt ttgatattga ccgacgcggc gttgaagttt tcaaactcca
    22321 gttcgcctac gccaccacca tcaacggtgt aagcccagcg ggcatcgaag gcgtcaacat
    22381 caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac
    22441 catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct gcaatacctg
    22501 ctttgtcatc ggcacctaac aaggttttac catcggtggt aatcagcgtc tgacccagta
    22561 gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa
    22621 tatcgccacc gcgatagttt tcaacaattt gcggattcac atttttgccg ctgcaatccg
    22681 gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg acgttagccg
    22741 gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc cccatctctt
    22801 cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg ggaacctgtc
    22861 tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc tcaagtagtt
    22921 tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag aaaagacaaa
    22981 tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat taccttaatg
    23041 aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg tttgccgtag
    23101 aattaccgcc ctcattaaga gtcaccaagg tggttaacca caaaccccgc atcggtaagc
    23161 catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga gttcgctttc
    23221 accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg tcattcccca
    23281 gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt ctggctgcgg
    23341 taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg gacgcatcat
    23401 gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga atactgtttt
    23461 ccaaagctac gcacttttcc cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg
    23521 catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc tgcggatggt
    23581 gcagctggaa accttcgctc aacgcaaacc gcatcagctc tctggtggtc aacagcaacg
    23641 cgtcgccatt gcccgcgcgg tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct
    23701 ctcagcactg gattacaaac tgcgcaagca aatgcagaac gagctgaaag cgttacagcg
    23761 taagcttggc attacattcg tcttcgtgac tcacgaccag gaagaagcac tcaccatgtc
    23821 ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc cgcgtgaaat
    23881 ctatgaagag ccgaaaaacc tgtttgttgc tgggtttatt ggcgaaatca atatgtttaa
    23941 cgccactgtc atcgaacgtc tcgacgagca gcgcgtacgc gccaacgttg aaggccgcga
    24001 atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg ttctgctgcg
    24061 cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag ggctgattgg
    24121 ttacgttcgc gagcgtaact acaaaggcat gacgctggag tcggttgttg aactggaaaa
    24181 tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact ttgaccactc
    24241 tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac tggctgatga
    24301 agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt
    24361 ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc gacgacgcca
    24421 gtttcgtcaa aatggtcttt acgctggata actacacgcg tctgctcgat ccgctctatt
    24481 ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc
    24541 tgggctatcc ttttgcctgg tttctggcga agctgccaca caaggtgcgt ccgctgctgc
    24601 tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac gggctgaaaa
    24661 ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc gttattgata
    24721 caccaattcg catcatgttc acgcccagtg cggtgattat cggtctggtt tacattctgc
    24781 taccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag ccgttgcttg
    24841 aggcggcgcg cgatctcggt gccagcaagt tgcagacctt tatccgcatc attatcccac
    24901 tgacgatgcc gggaattatt gccggatgtc tgctggtgat gctgccagcg atgggcctgt
    24961 tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac gtcatcaagg
    25021 ttcagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt acgctgacta
    25081 tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtctgctg aataagaagg
    25141 tgagcgaatt agatgattaa tcctttatat tcaatgcatt aagtttgttt ttgagtggtt
    25201 ttgccgttta aggtaacgac gttatagatc ctatcaaaat caatcaattc cattctggtt
    25261 ttggagaaag atttttcgag agcttcggtc tttaactaaa actgattgcg tataatccca
    25321 atacccctcc ccgactatct gaatggtcgg ggaggcactt caagcacaaa atatcatccc
    25381 tgcgccgttg attactctgc aggtgaagtc ggccactcaa tatcaggtgc agttgatgta
    25441 tcaacacgat tcaacaatac ccgatattta
//
LOCUS       DAOTRK010000064        25292 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.064, whole genome shotgun sequence.
ACCESSION   DAOTRK010000064 DAOTRK010000000
VERSION     DAOTRK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.064"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            44..850
                     /gene="rna"
                     /locus_tag="QIE41_002487"
     CDS             44..850
                     /gene="rna"
                     /locus_tag="QIE41_002487"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDS3531364.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQFGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            1080..1490
                     /gene="rnk"
                     /locus_tag="QIE41_002488"
     CDS             1080..1490
                     /gene="rnk"
                     /locus_tag="QIE41_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDS3531365.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            1846..2274
                     /gene="uspG"
                     /locus_tag="QIE41_002489"
     CDS             1846..2274
                     /gene="uspG"
                     /locus_tag="QIE41_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDS3531366.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(2395..3960)
                     /gene="ahpF"
                     /locus_tag="QIE41_002490"
     CDS             complement(2395..3960)
                     /gene="ahpF"
                     /locus_tag="QIE41_002490"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDS3531367.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVIGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(4131..4694)
                     /gene="ahpC"
                     /locus_tag="QIE41_002491"
     CDS             complement(4131..4694)
                     /gene="ahpC"
                     /locus_tag="QIE41_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDS3531368.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            5066..5812
                     /gene="dsbG"
                     /locus_tag="QIE41_002492"
     CDS             5066..5812
                     /gene="dsbG"
                     /locus_tag="QIE41_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDS3531369.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            6020..6922
                     /locus_tag="QIE41_002493"
     CDS             6020..6922
                     /locus_tag="QIE41_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3531370.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGIEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FMNRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLQEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            7069..8289
                     /locus_tag="QIE41_002494"
     CDS             7069..8289
                     /locus_tag="QIE41_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDS3531371.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YASHQRLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            8262..8891
                     /locus_tag="QIE41_002495"
     CDS             8262..8891
                     /locus_tag="QIE41_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDS3531372.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(8892..10052)
                     /gene="ybdL"
                     /locus_tag="QIE41_002496"
     CDS             complement(8892..10052)
                     /gene="ybdL"
                     /locus_tag="QIE41_002496"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDS3531373.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            10161..11249
                     /gene="hcxA"
                     /locus_tag="QIE41_002497"
     CDS             10161..11249
                     /gene="hcxA"
                     /locus_tag="QIE41_002497"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDS3531374.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSLNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFERPGAKHILFRGHCSESGVQQLAADSGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHFEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAICDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDNVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(11259..11456)
                     /locus_tag="QIE41_002498"
     CDS             complement(11259..11456)
                     /locus_tag="QIE41_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDS3531375.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(11584..13689)
                     /gene="cstA"
                     /locus_tag="QIE41_002499"
     CDS             complement(11584..13689)
                     /gene="cstA"
                     /locus_tag="QIE41_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDS3531376.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(13870..14283)
                     /gene="entH"
                     /locus_tag="QIE41_002500"
     CDS             complement(13870..14283)
                     /gene="entH"
                     /locus_tag="QIE41_002500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDS3531377.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTLDGQCVVGTELNATHHRPVSEGKVRGIC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(14286..15032)
                     /gene="entA"
                     /locus_tag="QIE41_002501"
     CDS             complement(14286..15032)
                     /gene="entA"
                     /locus_tag="QIE41_002501"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDS3531378.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(15032..15889)
                     /gene="entB"
                     /locus_tag="QIE41_002502"
     CDS             complement(15032..15889)
                     /gene="entB"
                     /locus_tag="QIE41_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDS3531379.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(15903..17513)
                     /gene="entE"
                     /locus_tag="QIE41_002503"
     CDS             complement(15903..17513)
                     /gene="entE"
                     /locus_tag="QIE41_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDS3531380.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNRAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(17523..18698)
                     /gene="entC"
                     /locus_tag="QIE41_002504"
     CDS             complement(17523..18698)
                     /gene="entC"
                     /locus_tag="QIE41_002504"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDS3531381.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAKGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPDQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLHKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSHENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            18887..19843
                     /gene="fepB"
                     /locus_tag="QIE41_002505"
     CDS             18887..19843
                     /gene="fepB"
                     /locus_tag="QIE41_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDS3531382.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIVAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIVQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(19847..21097)
                     /gene="entS"
                     /locus_tag="QIE41_002506"
     CDS             complement(19847..21097)
                     /gene="entS"
                     /locus_tag="QIE41_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDS3531383.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVICLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            21208..22212
                     /gene="fepD"
                     /locus_tag="QIE41_002507"
     CDS             21208..22212
                     /gene="fepD"
                     /locus_tag="QIE41_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDS3531384.1"
                     /translation="MSGSVAVTRAIAVPGLLFLLIIATALSLLIGAKSLPASVVLEAI
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            22209..23201
                     /gene="fepG"
                     /locus_tag="QIE41_002508"
     CDS             22209..23201
                     /gene="fepG"
                     /locus_tag="QIE41_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDS3531385.1"
                     /translation="MIYVSRRLIITCLLLITACVVAGIWGLRSGTVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            23198..24013
                     /gene="fepC"
                     /locus_tag="QIE41_002509"
     CDS             23198..24013
                     /gene="fepC"
                     /locus_tag="QIE41_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDS3531386.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(24010..25143)
                     /gene="fepE"
                     /locus_tag="QIE41_002510"
     CDS             complement(24010..25143)
                     /gene="fepE"
                     /locus_tag="QIE41_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDS3531387.1"
                     /translation="MSSMNIKQGSDAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSAMIGGMVACGGVLLRYAMASRKQDAVMEEHLV"
BASE COUNT         6150 a         6909 c         6314 g         5919 t
ORIGIN      
        1 ttaatagata gtattcagtg tatgtatgag ttccacaccc attatgaaag cattctggcg
       61 taacgccgcg ttgctcgcgg tttctctgct tcccttctct tctgccaacg ccgtagcgtt
      121 gcaggcaaaa cagtttggcg attttgatcg ctatgtcctg gccctctcct ggcaaaccgg
      181 attttgccag agtcaacacg atcgaaatcg taacgaacga gatgaatgtc gcctgcaaac
      241 cgaaacgacc aacaaagctg attttctgac cgtacatggt ctgtggccag gattgcctaa
      301 atcggttgct gcccgtggtg ttgatgaacg ccgctggatg cgcttcggtt gcgctactcg
      361 cccaatcccg aatctaccag aagcgcacgc cagccgaatg tgttcatcgc cggaaaccgg
      421 gttatcgctg gaaacagccg ctaaactaag tgaagtcatg ccaggagctg gcggacgttc
      481 ctgcctggaa cgctacgaat atgccaaaca cggtgcctgc tttggttttg atccagacgc
      541 atacttcggc acgatggtac gcctgaatca agaaattaaa gagagcgaag ccggaaaatt
      601 ccttgcggat aactacggta agacagtgag ccgccgtgac tttgacgccg cctttgccaa
      661 aagctgggga aaagagaacg tgaaagcagt taagctaacg tgtcagggta accctgcgta
      721 tttgactgaa attcagatct cgatcaaagc tgacgccatc aacgctccgc tttctgcaaa
      781 ctcattcttg ccacaaccac acccaggcaa ctgtggcaaa acctttgtga ttgataaagc
      841 aggttattaa tcctctgatt tacaaaagac gctaattcgt cttccataaa ccatgacgat
      901 taagatttat acataatgcg ctacattgag gaattccctg aatgtgacgc aaatcacttc
      961 aaagagtgag caacagtcag tatcatcatg tgagttaaac cctcgccgcc tgacggtgag
     1021 ggttttcttt tgggtatgtt tatctgcgac actcgcagta ccgacgacat ggagtaaaaa
     1081 tgtccagacc aactatcatc attaacgacc tggatgccga acgcatcgat attctgctgg
     1141 agcaacccgc ctatgctggt ttgccaatcg ccgacgcgtt aaacgcagag ttggatcgcg
     1201 cccaaatgtg ttcgccagaa gagatgccac acgacgtggt gacaatgaac agccgggtta
     1261 aattccgcaa tcttagcgat ggcgaagtgc gtgtgcgcac gctggtgtat ccggcaaaaa
     1321 tgaccgatag caatactcag ctttccgtta tggctccggt aggtgccgca ctgctggggc
     1381 tgcgcgttgg cgattccatt cactgggaac ttccgggcgg cgttgcaacc caccttgaag
     1441 tgctggaact cgaataccag ccagaagctg ctggcgacta cctgctttaa tcccatctgc
     1501 acaagcgata actcgctttg cccagaggat gcactcgcat cctctttccg ctcaaatatc
     1561 aaccagattt atcattttgc tcttaagcgc agcattctaa tctgaactgg ttattcatct
     1621 gcggaacctc atatgagcga agctctcggt atcactgcat tttatatatt catagccgca
     1681 attattgttg ctgcagtgct ataccttgaa caacgctggt agcgcctggt tttatttatt
     1741 ttccagctca atagcaaatt cagatcaaat cagcctcgca aattatagat tcataaccaa
     1801 taatgtgctg ttatgtatac atgcaaaaac aaggagagaa tgcttatgta caaaacaatc
     1861 atcatgccag tggatgtttt cgaaatggaa ttgagcgaca aagcggttcg ccacgctgaa
     1921 ttcctcgccc aggatgacgg agttattcat ctacttcacg tactaccggg gtcagccagc
     1981 ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa
     2041 gcagaagaac gtctgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaag
     2101 caacatgtcc gttttggtag tgtgcgggat gaagttaacg agttagcgaa agaacttgat
     2161 gcagatgtag tagtgattgg ttcgcgcaac ccatcgattt cgacccatct gttaggttct
     2221 aacgcctcaa gcgttatccg ccacgccaat ctgccggtgc tggttgtgcg ttaattgcca
     2281 gaataaatat cccgccctgc ccggagccat cccggcgggg ctttttattg ccataaaaaa
     2341 gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc
     2401 agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgccagt
     2461 ggcgatgatg atctgcttgt acggaaccgt cgtacagtca cccgctgcga acacgccttt
     2521 cacgttggtt tcgcatttcg catcaatgat aatctcgccc atgcggttac gttcgactgc
     2581 gccttcgagc cagttggtgt tcggcagcag accaatctgc acgaaaatac cggccagttc
     2641 gatgttgtga atatcgccgc tgacacgatc tcgatattcc agaccaatga ctttgctgcc
     2701 gtcgcctttc acttccgtag tttgcgcatt cagaataatg tcgacgtttt tcaggctgcg
     2761 cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcca gcagcgttac
     2821 gtgctcaacg atacccgcca ggtcgattgc cgcttccacg ccggagttac cgccgccgat
     2881 aaccgctacg cgtttgcctt taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc
     2941 tttggtgcga tactgatctt cgcccggaac gttcatgttg cgccattttg caccggtcgc
     3001 cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcagacc
     3061 accttcaacc gctgccggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc
     3121 gtattcatca acgtgaactt tcagcgctcc tgccagcttc tgcccttcag tcttcggtac
     3181 agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat
     3241 cagaccagta cggatgcctt tacgtgcgga gtaaattgct gccgctgcac ccgctgggcc
     3301 ggaaccgacg attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt
     3361 ttccgcgcca gtatcaattt tggcgacgat ttcagtcaac gtcatgcgac cctgaccaaa
     3421 ctctttcccg tttacgaata ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt
     3481 ctggaaggta cctccgtcaa ttgcagtgtg cttgatgcgc gggttcagta cgctcatcag
     3541 gttcagcgcc tgcaccacat ccgggcagtt gtggcaagag agcgagtaat aggtttcgaa
     3601 ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg
     3661 atgaccaccg gtccacaaca acgccagtac cagcgaggta aactcgtggc ccagcgggga
     3721 acctgcaaaa cgtggcccct ggttagaacc tgggttggtg atcaggaaag acggcttacg
     3781 caccggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc
     3841 caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac
     3901 aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat
     3961 gtttatctcc tgggctttaa acatcatcat gcaagcggcc tctgcggcta cctggatgca
     4021 gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag cacccgaaga attaggtgaa
     4081 tttccgaagt atttcgcgcc ggagttggta gcgcgaaaga cgaaggaaat ttagatttta
     4141 ccaaccaggt ccagagacgg agccagagtt gcttcacctt ctttccattt agccgggcaa
     4201 acttcacctg ggtgagaagc tacgtactgt gctgctttga ttttacgcag caggtcagac
     4261 gcgtcacggc caatgccttc agcggtaact tcgattgcct ggatgatacc ctgcgggtca
     4321 acaacgaagg tcgcacggtc agccagacct tcatcttcac gcatgttgtc gaagttacgg
     4381 gtcagggcgc cagtcgggtc gccgatcatc gcatatttga ttttagcgat ggtttcagag
     4441 ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag atactgcgta tacgtctacg
     4501 cccagtttct gcagttcttc gtagtggtca gcaacgtcac ccagttcggt cgggcatacg
     4561 aaagtaaagt cagccgggta gaagaagaag acgctccagc ggccttcggt atctttttcg
     4621 gtgatttcga tgaattcgcc gtttttgaat gcctggtttt taaaaggttt aattttggtg
     4681 ttaatcaagg acatctatac ttcctccgtg ttttcgatga gatgtaaggt aaccaatttt
     4741 tgccgattcg gctaatgcgt ttccattatc aaatcgataa gttttacctt acaaccttcg
     4801 taagacaact ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat ggcccccttt
     4861 ccaggtgacc gtgtaatctg aactaccctt tggtatcttc agcgcctgcc tgcaggctga
     4921 ggtgttgccc ttacaaatgc aacaacgaca tggattacaa caccctcata aacaaagggc
     4981 aatcacctga tctaagctct tacctatggc agtgataggt tatgcctttt acccgacttt
     5041 tgcactgact gaaaaggaca aattaatgtt aaaaaagata cttttactgg ctctgcttcc
     5101 tgcaatcgcc ttcgcagagg aacttcctgc cccagtaaaa gcgattgaaa aacagggcat
     5161 tacaatcatc aaaacgttcg atgctcccgg aggaatgaaa ggttatctcg gaaagtatca
     5221 ggatatgggc gtcaccatct acctgactcc agatggtaag cacgctatct ccggttacat
     5281 gtacaacgag aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa tttacgcacc
     5341 agccggacgc gaaatgtggc aacggatgga acaatcccac tggctcctcg acggtaaaaa
     5401 agacgcgccg gtcattgttt acgtcttcgc cgatccgttc tgcccatatt gtaaacagtt
     5461 ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg caattaagaa cattgttggt
     5521 tggggttatc aagccagaaa gcccggcgac agcagcggca attcttgcct ccaaagatcc
     5581 cgcaaaaacc tggcaagaat atgaagcctc tggtggcaag cttaagctaa acgtgcctgc
     5641 aaacgtaagt acagagcaaa tgaaagtgtt aagcgacaac gagaaactga tggacgatct
     5701 gggggcaaat gtcacaccag ctatctatta catgagtaag gaaaatactc tgcaacaggc
     5761 cgtggggttg cccgatcaga aaacgcttaa tatcattatg gggaataaat aaaatataat
     5821 tcatggatgg ttgaaatata tccctgcttt tcaaccatcc taaaaaagca tgaattgctc
     5881 gctctaccct aagtggctgt gctattttag aaaaacttaa tcctgagtta agcaaaaaaa
     5941 cataatacaa ataaatacaa cagcaacatt attgatcaag tgaatgattt atagaagaga
     6001 taatataacg gagtatgtta tggcgaatct ttatgaccta aaaaaatttg atctgaattt
     6061 actggtcata tttgagtgta tttatcaaca tctcagcatt agtaaagcgg cagaatcatt
     6121 atatataacg ccctctgctg ttagtcagtc gttacaacgt ttacgtgcgc aatttaatga
     6181 cccgttattc attcgctcag ggaaaggtat cgcgccgact acgacagggt taaatctgca
     6241 tcatcatctt gagaaaaatc tcagaggcat tgaacaaacg atcaatattg ttaataagtc
     6301 tgagctaaag aaaaatttta ttatctacgg tcctcagctt atttcttgta ctaataacaa
     6361 catgctaatc cgctgtctta gacaagatac ttcaatcgat atagaatgcc atgacatact
     6421 gctttcagct gaaaatgccg aagaactgtt ggttcaacgc aaaactgacc tggcaattac
     6481 actacaacca gtaattagcc gctctgtcat ctgcatgccg ttacatacta ttcgcaattc
     6541 tcttatatgt agcaacaaac atcctcgtat taccgatacc agtaactatg aacaaattat
     6601 cgccgaagaa ttcaccttgt tgatatctaa atcagcaggg attgacgaaa ttcaaatgga
     6661 catagatgaa cgttttatga accgtaaagt ttcatttcgc agttcttctt tgttgacgat
     6721 aattaatagt atttctgtta ctgacttatt gggcattgtg cccaccgaac tgtacgattt
     6781 acatcgtgat tttttgaagt tacaagagat aaaactggaa caaccacttc ctgcggttaa
     6841 actttatatt tcctataaca aggcttcgtt gaacaacctt gtcttctcac gattcataga
     6901 tcgtttaaat gatagcttct gatttattac cactgataaa tgttatatta cgcatcaatt
     6961 gattcattgt gtaaatataa aataatgtgc tctatcttgc ttcgtttacc ctataattag
     7021 cggattcatc tcacgagtca ccccgtaacg ttcagagagc gttttcagat gtctatttat
     7081 aaaatcccgc ttccactgaa tattctggaa gctgcaaaag aacgtattac ctggacactt
     7141 aacaccctac cccgcgtctg cgtttctttt tcgggtggta aagattccgg attaatgctg
     7201 catctgaccg ccgaacttgc ccgacaaatg ggcaaaaaaa tttgtgtttt atttatcgac
     7261 tgggaagctc agttctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc
     7321 gatgtcatcg aagagtttta ctgggttgcg ctcccgctta caacgcaaaa ttccctatca
     7381 caataccaac ccgaatggca gtgctgggaa cctgatgtcg aatgggtacg tcagcctccg
     7441 caagatgcga taaccgatcc tgactttttc tccttttacc agccaggcat gaccttcgaa
     7501 caatttgtgc gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc
     7561 ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa
     7621 cgttttgccg atgataagcc ctggaccact gccgcaccag gcggtcatag ctggtacatt
     7681 taccccatct acgactggaa agtcgctgat atctggacct ggtatgctag ccatcaacgc
     7741 ctctgtaacc cactgtataa tataatgtat caggcaggcg ttcctctacg tcatatgcga
     7801 atttgcgaac cttttggtcc ggaacaacgg caaggattat ggctctatca cgttatcgaa
     7861 ccggatcgct gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac
     7921 gccggacatg acaaccattt ttatggtcat cggaaaatcc tcaagcccga acatttagac
     7981 tggcaggagt atgcattatt gctactcaac agcatgccgg agaaaacagc tgagcattat
     8041 cgcaataaaa tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa
     8101 acccagcaag gggacattgg cgcgaaagat atcccctcct ggcggcgaat atgcaaagtt
     8161 ttactcaata acgattactg gtgtcgggca ttatcattta gcccgaccaa agcgaagaac
     8221 tatcagcgtt ataacgaacg aataaaagga aaacgtcagg aatgggggat actatgcaac
     8281 aacgattaac gcaggattta acccaatttc tcgccagctt gccggaagac gatcgcatca
     8341 aagcgattaa tgaaatccgc atggcgatcc atcaggttag cccctttcga gaagagcctg
     8401 tggattgcgt tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca
     8461 acgtggcacc acccgaaaaa aaactgctga aaaaatccat tgaaatagat ggttttaccc
     8521 aacccattgt ggtcacgcat acggataaaa atgccctgga aatcgtggat ggttttcacc
     8581 gccatgaaat tggtaaaggc tcatcatcgt taaaattacg cttaaaaggt tatttgccgg
     8641 tgacctgtct ggaaggtacg cgtaatcaac gcatcgccgc gactattcgt cataatcgcg
     8701 cccgtggacg tcaccaaatc actgccatgt cagaaatcgt ccgtgaactc agccagttgg
     8761 gatgggacga taataaaatt ggcaaagagc tgggaatgga cagcgacgag gtgctgcgcc
     8821 tgaaacaaat taatggcctg caagagttgt ttgccgaccg tcaatattcc cgtgcctgga
     8881 ctgtcaaata actacagctg gcgcaggcgt tcagctgctg ccagcaacgt cgattccttc
     8941 ttggcaaaac agagacgaat cagtttatgg gggaagggat cggcgcaaaa taccgacagc
     9001 gggatcgccg ctacaccgtg ctcccgcgtc agccactggc aaaactcaac gtcatccagc
     9061 gaagaaaccg cgctgtaatc caccagcaaa aagtaagtgc cttcgcacgg taaaatctcc
     9121 agccgactct cacttaaggc attcaccaga atatcgcgtt tttggcgata aaagtccggt
     9181 aacgcaagat aatgctcagg ttctgcacgt agcatatccg caagtgccag ctgtgccggg
     9241 gtattcaccg aaaaggtcag atactggtgt accttgcgaa tttcagcgct gatgggcgct
     9301 ggcgcaacgc aataacccac tttccagccg gtcatatgat aggtcttgcc aaatgaagaa
     9361 actgccaccg cccgctcacg cagctgcgga tgcgccagca cgctggcatg gccctgttgt
     9421 gaaaagttga tgtgctcgta aacttcatcg ctaatgacaa aaatctcgtg cccggcaatt
     9481 gcctgccaca aagcggcgaa atcagcctgc tgccagacag ttgcactggg gttatgcggg
     9541 gtgttgagga tcaccagtcg ggtgcgctcg cttaacaatg cggcaaattc ctgccagtcc
     9601 acgcgaaaat gcggtggttg cagtgcaata cgtttcacta ttccccccga aagcgcgatg
     9661 gcgggcgcgt aactgtcata gctgggatca aagcagatca cttcatcacc attgcgcacc
     9721 aatgcggtaa tcgctgcata taacgcttcc gtcgcccctg ccgttacggt gatatcgcta
     9781 tcggcatccg gttgatagcc atacagacgt tccgttttct gagcaatcgc ctcgcgcaag
     9841 gcctgcacgc cagtcatagg cgcgtattgg tttgccccct gggcaacgtg gtacgccagg
     9901 cgctcctgca aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag gttaattgcc
     9961 tggtgttgct gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt
    10021 ttgctttgtg gaatcagagg gttatttgtc atttttattg tacctgacga agttgcgtgt
    10081 tgaaggcact ataacacgat gctagtattt ggcaatcaag acgtttagat gtctaaataa
    10141 aacaataagg acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccagcta
    10201 actacttttc acatccaggc agtctcaatc acctgcatga ttttttcact gatgaacagc
    10261 tttctcgcgc ggtgtggatc tacggcgaac gcgccattgc agcggcgcaa acaaaacttc
    10321 cgccagcgtt tgaacggcca ggggcaaagc atattttgtt tcgcggtcat tgcagcgaaa
    10381 gcggtgtaca acaactggcg gctgactccg gtgacgatcg cagcgtggtg attggcgtcg
    10441 gcggcggcgc actgctcgac accgcaaaag ccctcgcccg ccgtctcggt ctgccgtttg
    10501 ttgccgttcc gacgatagcc gctacctgcg ccgcctggac accgctctcc gtctggtata
    10561 acgatgccgg acaggcgctg cattttgaga ttttcgacga cgccaatttt atggtgctgg
    10621 tggaaccaga gattatcctc aatgcgccgc aagaatatct gctggcgggg atcggtgaca
    10681 cgctggcgaa atggtatgaa gcggtggtgc tggctccgca accagaaacg ttgccgctaa
    10741 ccgtgcggct ggggatcaat aatgcgcagg ccatttgcga cgtcttgtta aacagtagcg
    10801 aacaggcgct gagcgatcag caaaaacaac agttaacgca atcattttgc gatgtggtgg
    10861 atgctattat tgctggtggt gggatggtcg gtggtctggg cgatcgtttt acgcgtgtgg
    10921 cggcggccca tgccgtgcat aacggtctga ccgtgctgcc gcaaaccgag aagtttcttc
    10981 acggcaccaa agtcgcctac ggaattctgg tgcaaagcgc cttgctgggt caggatgatg
    11041 tgctggcaca attaaccggg gcgtatcagc gttttcatct gccgactacg ctggcggagc
    11101 tggaagtgga tatcaataat caggcggaga tcgacaacgt gattgcccac accttacgtc
    11161 cggtggaatc cattcattac ctgccagtca cgctgacacc agatacgttg cgtgcagcgt
    11221 tcgaaaaagt ggaatcgttt aaagcctgac gtacacattt agcagcagcg cgcgccgcct
    11281 tttccaccgt agcgcgcgtc ctgccgctca cggaaaaact cttcgtaggt catcggcgtt
    11341 tgatcgggat ggttaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc
    11401 atcagcttcg ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatcaat
    11461 acctccggta aacaggttgc tgaaatattg ccggatacgg cgcgaacacc ttatccggcc
    11521 tggaattgct gagagtaagg ggaaccctcg gcactatgcc aacattcccc cctcactctg
    11581 actttagtgc gcgccttttg cctgcgccac gatctcctcg acattttccg gcattggctc
    11641 atacggcgtt tctttcgccg ttggcttcgg ctctttcaat gccgccagtg ccgtcttaat
    11701 cgagaacagt gccagaacca ccacgaccac cataaagaag atggttaacc cggcatcaag
    11761 acggttgttg aacaccagtt gcgccagttg tgactcagta tactgcgacg gaatattgcc
    11821 gctgtcgatc attgcctgga acttattagc aatcgccagg aagccgactt tcgcatccgg
    11881 gctaaacgct ttctgccagc ctgcggtcag ggtacaaatc agcagccagg ccgttggtac
    11941 cagcgccacc caggcgtaac gttgacgttt cattttgaac agcaccacag cacagagcat
    12001 cagcgccatc cctgccagca tctggttggc aataccaaac agcggccaca aggtgttaat
    12061 accgcccagc ggatcgacca cgccctgatg caggaagtag ccccacgcca gcacgcacag
    12121 cgccgttgcc agcaggttag caggcagtga atcggtccgt ttcaggcccg gagacaccac
    12181 gcccagcaga tcctgcaaca taaagcgcgc agcacgcgta cctgcatcca ccgccgtcag
    12241 aataaacagt gcttcaaaca gaatggcgaa gtgataccag aacgccacat ccatcatgcc
    12301 gcccagcgca ccgtgcagaa tgtaggccat ccccaccgcc agggtcggcg caccgcccgc
    12361 acgggaaatg atcgactgtt cacccacttc gctggcaatc tggtttagcg tatccggggt
    12421 aatcgcaaag ccccagctac tcaccacctg cgcggcagaa gcaaccacat ccgccgtccc
    12481 tgccggagcc agcaccgcca tcgggctgtt catggcgaag tacacacccg gatcgatgat
    12541 acaggcggaa accagcgcca taatcgctac gaaggattcc attaacatcc cgccgtagcc
    12601 gataaaacac gcctgccctt cgttcgccag catcttcggc gtggtgccgg aagagatcag
    12661 cgcatggaag ccagacaccg cgccacaggc gatggtgata aacaggaacg ggaacaggtt
    12721 accggtccat accgggccag taccatcaac aaatttggtc agcgcaggca tggtcagcgt
    12781 cgggcgcata atcaaaatgc ctaccgccag accgacgata gtcccgattt tcaggaaggt
    12841 tgagaggtag tcacgcggag ccagcagcag ccacaccggc agcaccgccg ccacaaaacc
    12901 gtaacccacc agcatccagg tcagctgcac gccggtaaag tcaaagtacg gtgcccaggt
    12961 cgggctttct gccacccagc cgccagagat aatggcgaaa atcaggaaca ccagaccaat
    13021 caccgacact tcgccaatac gccccggacg caaatagcgc aggtagatcc ccataaacag
    13081 cgccagcgga atggtgaacg caacggtata cgttccccac gggctatgag tcagggcttt
    13141 caccacgatc atcgccagta ctgcaaggat aatgaccatg atcataaagc aggccaccag
    13201 cgcaatcacc ccggcggttg gccccatctc ttctttgacc agctcaccca gcgagcgacc
    13261 gtcacggcgc gtagaaacaa acagcaccat gaaatcctgc accgcaccgg cgagaaccac
    13321 cccggcgagc agccagatca tccccggcag gtagcccatt tgcgccgcca gcaccggccc
    13381 aaccagcgga cctgctccgg caatggccgc aaaatggtga ccgaacagca ctttcttgtc
    13441 cgtcggcaca tagtccagcc cgtcgttatg gcgcacagct ggcgtcatac gcgtcgggtc
    13501 aaccgccagc acatttttgg cgatatacag gccataaaaa cggtaagcga tcagatagat
    13561 acagaccgac gccaccacaa tccacagcgc gttgatctgt tccccacgat ttaaagcaat
    13621 gtatcccaga gcaaatgctc ccattacaga gagcactgtc cagacgaggt atttccctga
    13681 tttgttcata gttgttatcc gtgtgcgtgt ccatagagat gttacatttt gtttctataa
    13741 catctttgta caatttaaca acacagcaac catgtaaatc ggagttgaaa ccaatattta
    13801 acttaacaat gttaactcga tcactccgtt aaccgagttt tcctgcaatc tcatttaatt
    13861 ctgtcgggct catcccaaaa ctgccgtacc cagccgacag gtgcagcaac gccgcccctg
    13921 ttcatcgaaa acaacgattt cccagctctg attttgccgc ccaagatgca gcggctggca
    13981 gatgccgcgt accttgcctt cagacaccgg gcgatgatgt gtggcgttaa gttccgtgcc
    14041 caccacacac tgtccgtcga gggtcatcat aaatccggcc atcgatccca gcgtttccgc
    14101 cagcgccgcc gacgcgccgc catgtagtaa accgaacggc tgatgagtac gggtatcgac
    14161 cggcatttcg gcttcgagta catcatcgcc cagacgggta tacacaattc ccagatgcgc
    14221 caccattgtg ttatcgctgg tggcgttcag ctcgtcgagc gttaaatggc gtttccagat
    14281 catgcttatg cccccagcgt cgagccgcca tctaccacaa tatcctgtag cgtgatatgg
    14341 ctggcgaggt cagaggcgag gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt
    14401 ttccccagcg gaatgccgag tttaaactgc tcgccaaagc cgcgaatacg ctgctcttcg
    14461 gcgtcatcgc tcacccacag cgtgcgttgc atatcggtgt cggtggagcc tggcgaaacc
    14521 acattacagc gcacgccgct acccgccagt tccagcccaa cgctcaacgc caggcttttc
    14581 agcgccgctt tcgacgcgcc ataagcactc ataccaatgc gcggcgtgtg tgcggcgtcg
    14641 gacgcaacag taacaatcgc cccgccccgc tgacggcgaa actggttcat ggtctgctgg
    14701 aacaggttaa acgcaccgcc gacattgacc gcaaaagtct gctgccagtc ctccttactg
    14761 agctgatcgg tcgcgcccat gcgcaaaatt cccgccgcat tgaccagcac gtccagtcgc
    14821 tccgtttcag ctaacagtcg ctgacatacc tgcgccacct gcgcagcatc ggcaacatcc
    14881 attacttcgg tcgcaaaagg atattgctcc tgagtaaacg cttgatcaaa acctgtaact
    14941 ttcgcccccg cctcaacaaa tgccagcgcc gtagcatagc cgataccttt ccccgcgcca
    15001 gttacccaga catttttacc gctgaaatcc attatttcac ctcgcgggag agtagcttcc
    15061 accaggcgtc gatggtcggg tttttcgcca gcatgacaaa gtcgatatcg ccatgcactt
    15121 tgcgccagcg cgccgccagc gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt
    15181 tgtcgtcatc gaacggttca tcggactcgt ccagcaacgg caggatcacc tcacgcagcg
    15241 ccgctttgct ggcggggata ggtgctggca gtaattcttc ggtcatcacc acccggccag
    15301 aacgtccggc cacatatttc agcgacatca aatgctcgtc acggctgaaa tcagccagcg
    15361 catccgccac cataaacggt ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac
    15421 agccaatgtg ggcatatacc ccggtaataa tcagctggtt acgtccactc tctttgagca
    15481 tttgctccag cggggaacga tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt
    15541 cggcatctgg cgtcaggcga tccaccacct tttgctgttc cggcgaacgg gtcaggccag
    15601 gcccccacat atcattcaac agcgcccgat cttcatcgct ctgctctttc ggctgggcgg
    15661 tgtaataaac cgggatattg tgctgtttgc agtagtcgcg tagtgccgca atattcgcga
    15721 tcacctgctc catcatcggg cagttctcgc cccagaagct gacaaaatag tcctgcatat
    15781 catggattaa caacgcggca cgttgcggtt caaaggccca gtcaacttta ttctgcggaa
    15841 tatcgtgaga ctccggcagt gcgtaagcct gtaattttgg aatagccatc gtgttctctc
    15901 cttcaggctg atgcgcgtga cgccagccac tgacgtaatt gttttttatc gactttcccg
    15961 accgccgtca gcggaagtga atccacacac tccacgcgat ccggtaattt aaattcggca
    16021 ataccctgtt cacgcaggaa acgacgcacc tgcaccgcgc gtagaggctc ttttaccacc
    16081 agataagcac agcttttttc gcccatcagc tcatcttcca tgctcaccag tgcggcatag
    16141 atcaccgccg gatggcgcag cagcaggttt tcgatctctt cggcagcgat cttctcgccg
    16201 ccacggttga tctgatcttt ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata
    16261 gagatcagat cgccggaaca gtaaaaaccg ttggcatcaa aggcactggc attgtgctgc
    16321 gggcttttgt aataaccgcg gaaggtgtac ggcccgcgcg tcatcaggcg tccaacttcc
    16381 ccttgcggca gtggatttcc ttcggcatcg gcaacccaca cttcatcgtc cgggcacatt
    16441 gggtaaccct gggtatggat aattttctcc gcgctatcat caagacgggt gtagttcacc
    16501 agcccttccg ccatgccaaa cacctgctgc aactggcagc caatctcagc gggaatacgc
    16561 gccgcaagcg tggcagaaag acgtgcgccg ccgacctgta acagtttcag cgaggcaagc
    16621 tgcgcccggc tttcgccttc ggtcagcgcc tgtaaccaca ggctgactgc gggtggcacc
    16681 agcgcggtga cgttcacctg atgtttttca atcaatggga aacagagcgt ggcgctggga
    16741 tcggccgcca gaacgaccgt tcctccggca agaaagacgc ccagcgatcc cggcgaactc
    16801 atggcgtagt tatgagccgc cgggatcgcg cacaggtagc gtgtctgttg tgtgaactga
    16861 caaatctcga cgctacgacg cacgctgtag tagtagtcgt tatgagtgcg cgggatcagt
    16921 ttcggtgtgc cggtggtgcc gccggaaagc tggaaatagg ccacttcatc agcaggtgac
    16981 ggcgtggcag taaaatcctc agccggatgg ttaatcgcat cctgcaaatt atgctcaccg
    17041 ctgtcgttgt gcagttgcac cacgcgaatg gaggaatgct ctgcaacaaa tgtattgagg
    17101 aaatcatccc cgctaaacag cgcatgctgg cgatcggcaa tcagtaacgc cggttcaatc
    17161 tggctggcat aggcgttcag ttcactacgc tgatggctga acagcgccag caccggcgca
    17221 acgcccagct tcagcagcgc gaaaaaggta atatacaatt cagcgacgtt acccagctgt
    17281 accagcgcgg tttcaccagg tttaatgccc tggcggcgca aactacaggc gaggttatct
    17341 gccgcccgat tcagctcccg gtaactcaac tgccgctcgc cgtcgatgac cgcgatgctg
    17401 tcgcttgcgg catgacgggt cagaatgtcg gtcagcggca aatcctgcca gtagcctttt
    17461 tcccgatagc gacgggcaaa ctcttccggc cagcgggtga atggaatgct catcctcact
    17521 ccttaatgca atccaaaaac gttcaacatg gtagaaagtt tgacgcccgt ttcgcgccac
    17581 tcacccaacg gtgacgacgc aggcacaatc cccgctccgg caaacagacg cacctgattt
    17641 tcccgcagct tcgcgcagcg gatggtcacc acccactcgc cgttaccttc gctgtcacac
    17701 caacccacaa tgccgccaaa cagttcgcgg tcgaacggct ccagttcagc aataacctgg
    17761 gtcgcggcct gatgcgggaa accgctcagc gcgggggtcg gatgcagcag acaggccagc
    17821 gtcagtgcgt tctcatgcga attcgcttta ccttcaaagg gagttgcgag atgccacagc
    17881 gtcggcgtgg tgatcagttg tggagaagaa gggacgtgta actcactact tcgttcgcgc
    17941 agtacctctt tcatcgcctg agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc
    18001 agcagacgat tacccgcttc gcgatcgagc acttcatccg gctgacgacg cgcggaaccg
    18061 gctaacggaa tggagctaaa acgctcgccg tctttatgta gcagcagttc cgggctggcc
    18121 cccagcagaa cgccaccatc agccagcggg acatggaagt tgtaactaac cgggttttgc
    18181 gcaatcaacc gttccagcaa tacgccacta tcaatggcgg cgtcagtggt gatatcaatc
    18241 aaccgtgaca acaccacttt gtcgacctgc ggcgtggcgg taagtgcggc ggcgcgagca
    18301 accatctgtt caaacgtggt ttgatccgga attgcctgac gttccaccac gttcagcgac
    18361 tggctgcggg tgaaacggcg tgcggaagct tgtttttcct ggcgggagaa cgactgccag
    18421 gattcaggaa tatacagcga tgaaggctga cgtggatcga agggaatcgc cccgaccatc
    18481 accggatttt tgatgccctt cgctttggca tcggcaaaca gcgcggcgag tttttgctgg
    18541 aagggactgt cgggcgaatc cccgttcaca gccggttcat cgaagcgggc gaaacatcct
    18601 gacgtcgtaa aactgcggta cggcgacata aagaaaaagc gattgggcgc aagtgttgcc
    18661 atggtctgct gtacttcctc agccagtgac gtatccatat catcctccac aaaatgataa
    18721 aggctttaat aatgattatc atttatattt tcggtcgcta acctaaaagc aaacgcgcac
    18781 tatgtcaact cttgaggtaa cgcaatctgc aactcggggt tgcatctggt tgtggttaca
    18841 atgaaaatga gaagcattaa cttcattaat aacaggaagc tgacttgtga gactcgcccc
    18901 gctctaccgc aacgcccttc tattaacagg acttttgctt tcaggaatag tcgcagttca
    18961 ggccgccgac tggccgcgtc agattactga cagccggggc actcataccc tggaaagcca
    19021 gccgcagcgt attgtttcca ccagcgtcac cctgaccggc tcactgctgg cgattgatgc
    19081 tccggtgatt gccagcggcg cgaccacgcc gaataaccgc gtcgcggatg accagggctt
    19141 tttacgccag tggagcaagg tggcgaaaga acgcaaactg caacggctct atatcggcga
    19201 accgagcgcc gaagccgttg ccgcgcaaat gccggatctg attttaatta gcgcaactgg
    19261 cggggattcg gcgctggcgc tgtacgatca gctttctacc atcgccccaa cattaatcat
    19321 caattacgac gacaaaagct ggcaggcgct gttaacgcaa cttggcgaaa ttaccgggca
    19381 tgagaaacaa gcggcagagc ggattgcaca gtttgataag caactggcgg cggcgaaaga
    19441 gcaaatcaaa ttaccgccgc agccggtcac tgccattgtc tataccgccg ctgcacacag
    19501 tgccaatctc tggacgccgg aatcagcaca agggcagatg ctggaacaac tcggctttac
    19561 gttggcgaaa ttgcccgcag ggttaaacgc cagccaaagc cagggcaaac gtcatgatat
    19621 cgttcagctt ggtggggaaa atctggctgc agggttaaat ggcgagtcac tattcctgtt
    19681 cgccggtgat cagaaagatg ccgatgctat ttatgctaat ccgctgctcg cgcacctgcc
    19741 tgcagtacaa aacaagcagg tttatgcgct gggaaccgag acgttccgac tggattacta
    19801 cagcgccatg caagtgctgg aacgacttaa ggcgctgttt taagcattaa ctgtcggacg
    19861 ctgtcacctg cggcggcgtc tggcgaaaac gtcgcaactc caccagcacc agcaataaca
    19921 acacaccgat aatcaacaaa ccaaaaccgc tcgcgcttgc ggaagcaacc ggtgtcatca
    19981 tcgctcccag accgcctaac agcgccgcac ctatggcatc gcccgtcacg ttttgcgccg
    20041 tccacaaacc gttaatccgc cctaacatcg cttccggggt ttgcgtttgc agcattgtgt
    20101 attgcagcaa cgagctgacc gcgctcagcc agccgaatag cgccagacaa atcacgccta
    20161 aaatccacat cggcatcagg ccaaacagcc caatggcgag gaacgatccc agcgtggaga
    20221 gcagcatcaa taaccctggt cgcgcgctat gtgccagctt cccgctggtt aacgcgccaa
    20281 tagccgcgcc gagcgggatc gccgcataga gaaaaccaat ctgtgccgcc gacatctgcc
    20341 agttgtcagc cagcgccgga tacagtaccc gcaccgcgct cgccatcgtc aataaaccac
    20401 ccagcagcgc aatccctccc accagcgggc tggcgagcag aaaacgaaat cctgccagta
    20461 atgatttcaa cggatgctca cgcggctgag gtggcggtgg caacgccgga aggcttaata
    20521 acggcagcaa ggtaataaac gtgcccgccg ccgccagccc gtagttccag gctacgccgc
    20581 cggtcgccag caataaaccg ccaatcatgg gcgaaatcac cgaccccagg cgcacggtca
    20641 acatggtgat cgccccggcc tgcattaagt tttcacgccc taccagtgca ggtgtcgccg
    20701 ccagtagcgc cgtaacgcca agtgatgcga aaaaaccatc ccataaacca agtaaataga
    20761 ttgccaacaa tgacggctcc ggcagcagtg cattaaggca cagtccaatg aagccaatgc
    20821 cacaggtgcc gcgcgccagc aaaatcactt ttttgcgttc atagcgatcc gccagcacac
    20881 cgccgaccat caggccaaca aacatcgcgc cgccggtcag cgtcaccgaa agccccacct
    20941 gccaggtaga atgcgtcatc atctggatct gcaccggcac cgcgacgccg agcaaaccca
    21001 gagacacaat tgagataaaa cgagcgagga atactgcgcg aaacgccggg tgcgttttca
    21061 acaggctgag gttaagcagc caggattgtt tattcattac aatgccttgc catcaaatat
    21121 tttaaatacc tattcttagg ctgcacatgc taacatatcc aaataagatc gataacgata
    21181 attaatttca ttatcatgga agttcgtatg tctggttctg ttgccgtgac acgcgccatt
    21241 gccgtgcccg gattgctgtt cttactgatt atcgcgacgg cattaagcct gctcattggg
    21301 gcaaaatcac tccccgcttc cgtagtgctg gaggccatct ccggcacctg ccagagcgcc
    21361 gactgcacca tcgtgctcga cgcgcgactg ccgcgcaccc ttgccggttt actggccggc
    21421 ggtgcgcttg ggcttgcagg ggcgctgatg caaaccctca cccgaaaccc acttgccgac
    21481 cccggcttgc ttggcgtgaa cgccggagcc agctttgcca ttgtgctggg tgcggcgctg
    21541 tttggttact cttccgcaca ggaacaactg gcgatggcct tcgccggggc gctggtggcc
    21601 tcattgattg ttgcctttac cggcagccag ggcggcgggc agttaagtcc ggtgcgttta
    21661 accctggcgg gcgtggcact ggcggcggtg ctggaagggc tgaccagcgg catcgccctg
    21721 cttaatcctg acgtctacga tcagttgcgt ttctggcaag ccggttcgct ggatattcgc
    21781 aatctacata ccttaaaagt ggtgctgatc ccggtgctga tcgccggagc aaccgcgcta
    21841 ttactgagcc gcgcgctgaa cagtttaagc ctcggcagtg acaccgcgac ggcgctgggt
    21901 agtcgcgtgg cgcgcacaca gttgattggt ctgctggcga ttaccgtgct ttgtggtagt
    21961 gcgacggcgg tagttggccc gattgccttt attggcctga tgatgccgca tatggcacgt
    22021 tggttggtgg gtgccgatca tcgctggtcg ctgcccgtca cgctacttgc cacccctgcc
    22081 ctgctgctgt ttgccgatat catcgggcgg gtgattgtgc ccggcgagtt gcgcgtttct
    22141 gtggtcagtg cctttattgg cgcaccggtg ctgatcttcc tcgtgcgacg taaaacgcga
    22201 ggtggcgcat gatttacgtc tctcgccgat taatcatcac ctgtttgctg ctaattaccg
    22261 cctgtgtggt tgcaggtatc tggggattac gcagcggtac cgtcacgctg gaaacctcgc
    22321 aggtattcgc cgcgctgatg ggcgatgcgc cgcgcagtat gacgatggtg gtcaccgaat
    22381 ggcgtttacc acgcgtgctg atggcgctgt taattggcgc ggctctgggc gtcagcggcg
    22441 cgatttttca gtcgctgatg cgtaacccgc tcggcagccc tgacgtaatg ggctttaaca
    22501 ccggggcgtg gagcggcgtg ctggtggcga tggtgctgtt tggtcaggac ctgacggcta
    22561 tcgccctggc agcaatggtg ggcggcattg tcacctcgct gctggtctgg ctgctcgcct
    22621 ggcgtaacgg catcgacacc tttcggttga ttattatcgg catcggcgtt cgcgccatgc
    22681 tggtggcctt taatacctgg ctgttgctga aagcgtcttt agaaacggcg ctaacagcag
    22741 gtttgtggaa tgccggatcg ctcaacggcc tgacgtgggc aaaaacctcg ccttccgcac
    22801 ccatcattat attgatgctc attgccgccg ccttactggt acgccggatg cgcttgctgg
    22861 aaatgggcga tgataccgcg tgtgcgttgg gcgtcagcgt cgaacgttcg cggctgttaa
    22921 tgatgctggt ggcagtggtg ctcaccgctg cggcaacggc gcttgccggg ccgatttcct
    22981 ttattgcttt agtcgcgccg cacattgccc gacgcattag cggcaccgct cgctgggggc
    23041 tgacccaggc ggcgctgtgc ggggcgctgt tactgctggc ggccgatctc tgcgcccaac
    23101 aactgtttat gccgtatcaa cttccggttg gcgtcgttac cgtcagcctc ggcggtattt
    23161 accttatcgt cttgttaatt caggagtctc gcaaaaaatg accgaatcag tagcccgttt
    23221 gcgtggcgaa cagttaaccc tcggatatgg caaatatacc gttgcggaaa atctgactgt
    23281 agaaattcct gatggtcact tcacggcaat tatcgggcca aatggctgcg gtaaatccac
    23341 gttactgcgt accttaagcc gcctgatgac gcctgctcac gggcatgtct ggctggatgg
    23401 cgagcacatt caacattacg ccagtaaaga ggttgcacgc cggattggcc tgttggcgca
    23461 aaacgccaca acgccgggag atatcaccgt gcaggagctg gtggcgcgtg ggcgttaccc
    23521 gcatcaaccg ctgtttactc gctggcgcaa agaagatgaa gaagcggtaa cgaaagcgat
    23581 gcaggccacg ggaatcactc atctggcaga tcaaagcgtg gatacccttt ctggcggaca
    23641 acgccagcga gcgtggatag caatggtgct ggcccaggaa acggcaatta tgctgctcga
    23701 tgaaccaacg acctggctgg atatcagtca tcagattgat ctcctggaac tgttaagcga
    23761 actgaaccgc gagaaaggct ataccctggc ggcggtgctg cacgatctta atcaggcctg
    23821 tcgttacgcc agccatttga ttgcattgcg ggaagggaaa attgttgctc agggagcgcc
    23881 gaaggagatt gtcactgctg aattgatcga gcgcatttat ggtctgcgct gcatgattat
    23941 tgacgatccg gtagccggaa ctccgctcgt ggtgccgctc ggacgaacgg caccttcaac
    24001 tgccaagatt taaactaagt gctcttccat cacggcatcc tgttttctgg atgccatcgc
    24061 atagcgcaac agcacgccac cacaagccac catcccgccg atcatcgcag aaaggatcac
    24121 aataatcgcc ttacccggac cgtctttttt cactggcaat gacgggctta actgatattt
    24181 aaacggcgta aaattcacat cgttgatatt tgcttttgtt agctgctcaa caagatactg
    24241 gcgattacgt aattcaccgt tcagttccgc aacgtcagta accgcttttt caatttccag
    24301 tttgcgttca ataccgtctg cgccgagaga aatagagaaa tcggggtcat ctttaacggc
    24361 ctgaccatta ctgtaaacgg gctttttaat tcctgccgcg ttagcaatgt cgagtgaata
    24421 attgaggcgc tgaatgtttg catcaagttg atttttcgtt ttaatgcggt cctgagccag
    24481 tttttctttt tcaaactggg ttttgatctc cagtttatta cggacgtttt ctatcgactc
    24541 tttcaccacc aacgcagaga tataatcgat atacccgctc aaaacggtct gcgcctcttc
    24601 gctggttggc gcggtaaaac ttagcgtcca ggaggtatac agtgacggtt catctttttt
    24661 cttactggca ttgtcatcaa ccgctttcat tttttcgctc aatgcgacaa ttgcgcgatg
    24721 caaatccagt tcgtcgattt tcgcctcttt taattggtcc atcacataag gtgttgagcg
    24781 caggtactct tccagcaagc taaccgactg aaacttcttg ataaacaggt taaatgcttc
    24841 tgtacgatca attttgatat ccagatccag cacacgaagc ttagtgaatg ttttctcaag
    24901 cgcctgccac tgaacaggtt ctggaggtgt gacaaccgcc gcgctggtcc atttttgcgg
    24961 caggatgaaa gagatcagta aacctgcgca ggcaaacgca aaaacgaccg ccatgaccgt
    25021 ttttttggcc cgccataaaa cttcaattag attaagtaaa tcaatttcat tattactggg
    25081 cgacgccaga ggatactcgg gaaaatgagc gtcacttccc tgtttaatat tcattgatga
    25141 catggggtat aacctgaaaa tgagtccaat agggggaaat ctccacggcg ctcactttac
    25201 cagttggtga gaattatccg aagctgaagt ttgtaaattc cttccactga atgcaacatt
    25261 cactctattt aagagaaata tccaccaaac tt
//
LOCUS       DAOTRK010000065        24559 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.065, whole genome shotgun sequence.
ACCESSION   DAOTRK010000065 DAOTRK010000000
VERSION     DAOTRK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.065"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="QIE41_002511"
     CDS             complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="QIE41_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDS3531388.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAQSATFVV
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PVLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGMLKRQALKYALGL"
     gene            1474..2898
                     /gene="miaB"
                     /locus_tag="QIE41_002512"
     CDS             1474..2898
                     /gene="miaB"
                     /locus_tag="QIE41_002512"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDS3531389.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3051..4091
                     /locus_tag="QIE41_002513"
     CDS             3051..4091
                     /locus_tag="QIE41_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDS3531390.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4088..4555
                     /gene="ybeY"
                     /locus_tag="QIE41_002514"
     CDS             4088..4555
                     /gene="ybeY"
                     /locus_tag="QIE41_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDS3531391.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4645..5523
                     /gene="corC"
                     /locus_tag="QIE41_002515"
     CDS             4645..5523
                     /gene="corC"
                     /locus_tag="QIE41_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDS3531392.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5548..7086
                     /gene="lnt"
                     /locus_tag="QIE41_002516"
     CDS             5548..7086
                     /gene="lnt"
                     /locus_tag="QIE41_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDS3531393.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNVFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLAVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7483..8391
                     /gene="gltI"
                     /locus_tag="QIE41_002517"
     CDS             7483..8391
                     /gene="gltI"
                     /locus_tag="QIE41_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDS3531394.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8561..9301
                     /gene="gltJ"
                     /locus_tag="QIE41_002518"
     CDS             8561..9301
                     /gene="gltJ"
                     /locus_tag="QIE41_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDS3531395.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9301..9975
                     /gene="gltK"
                     /locus_tag="QIE41_002519"
     CDS             9301..9975
                     /gene="gltK"
                     /locus_tag="QIE41_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDS3531396.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            9975..10700
                     /gene="gltL"
                     /locus_tag="QIE41_002520"
     CDS             9975..10700
                     /gene="gltL"
                     /locus_tag="QIE41_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDS3531397.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10818..11753
                     /gene="rihA"
                     /locus_tag="QIE41_002521"
     CDS             10818..11753
                     /gene="rihA"
                     /locus_tag="QIE41_002521"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDS3531398.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLNPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGLVDLLADRLKFYA"
     gene            complement(11798..12280)
                     /locus_tag="QIE41_002522"
     CDS             complement(11798..12280)
                     /locus_tag="QIE41_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDS3531399.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            12515..15097
                     /gene="leuS"
                     /locus_tag="QIE41_002523"
     CDS             12515..15097
                     /gene="leuS"
                     /locus_tag="QIE41_002523"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDS3531400.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            15112..15693
                     /gene="lptE"
                     /locus_tag="QIE41_002524"
     CDS             15112..15693
                     /gene="lptE"
                     /locus_tag="QIE41_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDS3531401.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            15693..16724
                     /gene="holA"
                     /locus_tag="QIE41_002525"
     CDS             15693..16724
                     /gene="holA"
                     /locus_tag="QIE41_002525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDS3531402.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLSENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            16726..17367
                     /gene="nadD"
                     /locus_tag="QIE41_002526"
     CDS             16726..17367
                     /gene="nadD"
                     /locus_tag="QIE41_002526"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDS3531403.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            17391..18002
                     /locus_tag="QIE41_002527"
     CDS             17391..18002
                     /locus_tag="QIE41_002527"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDS3531404.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            18262..18579
                     /gene="rsfS"
                     /locus_tag="QIE41_002528"
                     /gene_synonym="rsfA"
     CDS             18262..18579
                     /gene="rsfS"
                     /locus_tag="QIE41_002528"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDS3531405.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            18583..19050
                     /gene="rlmH"
                     /locus_tag="QIE41_002529"
     CDS             18583..19050
                     /gene="rlmH"
                     /locus_tag="QIE41_002529"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDS3531406.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            19081..20982
                     /gene="mrdA"
                     /locus_tag="QIE41_002530"
     CDS             19081..20982
                     /gene="mrdA"
                     /locus_tag="QIE41_002530"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDS3531407.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            20985..22097
                     /gene="mrdB"
                     /locus_tag="QIE41_002531"
                     /gene_synonym="rodA"
     CDS             20985..22097
                     /gene="mrdB"
                     /locus_tag="QIE41_002531"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDS3531408.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            22108..23196
                     /gene="rlpA"
                     /locus_tag="QIE41_002532"
     CDS             22108..23196
                     /gene="rlpA"
                     /locus_tag="QIE41_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDS3531409.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTNSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPATTPATTPA
                     MVTPQATSQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            23336..24547
                     /gene="dacA"
                     /locus_tag="QIE41_002533"
     CDS             23336..24547
                     /gene="dacA"
                     /locus_tag="QIE41_002533"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDS3531410.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
BASE COUNT         5915 a         6401 c         6482 g         5761 t
ORIGIN      
        1 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gtatattcaa tgtcgaatgc
       61 gatgttgaca cgtcttatcc ttcaaagtcg gatgcgacgc tgccgcgtct tatccgacct
      121 acggttggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
      181 tgacgtttca acatgccagc acgctccgcc gccatcaaac caagattacg cacaaaacgc
      241 agtggtggca gattattgct gaatccggca tagaacagat ccataccgct ctgcataatg
      301 aagttatccg ccatgcgccg catctggtaa cgcttgagga tcggataact ggcccacgct
      361 tcgccatagc tgcgagcgtt gaccagaacg tcaatcaggg catcgacatc acgataacca
      421 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
      481 agccccggct gcacatactg caacgcatgg cggcgcgtca gcggaaaagc gccagcggca
      541 agcggtgtta catacccaag acgcgacggg aaatgcttcg cgatttccgc ctgtaactgc
      601 gccatattca tattctgcaa ctggcgaatg cgtgccggag agtcatacca caccagcgat
      661 gcccagttat caaacaacgg cagaaacgca cgaggtccat ccggggtaaa ttgctgccag
      721 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatta acatacacga ctgtgcatac
      781 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gggaatttgc gccgtcggca
      841 ccaatcacca gcttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
      901 ttatggcgat gcagcgcaat cagcgagcct ggcacacgta acgttacttt cggatgcgct
      961 tccagcgcct gccacagcgc ctgttgcaga acagtgtttt ccaccatgta gccaagcaca
     1021 ggcagcttta attcggctgc atcaaacacc acatgcgccg tttcccactc ccaagtttcc
     1081 agtctgcggt aaggatggca acgcatagcc tgtaccgcgt cccagacgcc taaaccttta
     1141 agcaatgata ccgaagccgc actgatcgcc gagatccgca cgtccggctg gctatcaaca
     1201 acaaacgtcg cagattgggc atgttcgata accgttaccg aaaatccgtg ctgagccagt
     1261 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
     1321 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttacccct
     1381 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
     1441 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
     1501 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
     1561 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctgctgct gaacacctgc
     1621 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
     1681 aaagagaaga atccagacct gattatcggc gtcggcggct gcgtggcatc gcaagaaggc
     1741 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
     1801 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
     1861 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gtgccgaagg gccgaccgcg
     1921 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtagt gccttacacc
     1981 cgtggtgaag aggtaagccg tccgtccgac gatattctgt ttgagatcgc ccagcttgcg
     2041 gctcagggcg tgcgtgaagt caacctgctc ggtcagaacg tgaacgcctg gcgtggtgaa
     2101 aactacgacg gcaccaccgg atcgtttgcc gatctgctgc gtctggttgc tgcgatcgac
     2161 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
     2221 gaagtgtacc gcgacacgcc ggagctggtg agcttcctgc atctgccggt acagagcggt
     2281 tccgatcgca ttctgaacct gatgggacgt acccatacag cgctggagta caaagcgatc
     2341 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt catcgttggc
     2401 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
     2461 ttcgacatga gctacagctt tatcttctct gcacgtccgg gtacgccagc cgccgatatg
     2521 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctaca agaacgtatt
     2581 aatcagcaag cgatggcatg gagccgccgg atgctcggca ccacccagcg tattctggta
     2641 gaaggtacat cgcgtaagag catcatggag ctttccggtc gcactgaaaa taaccgcgtg
     2701 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
     2761 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
     2821 gtggcagaaa caccggaatc ggtcattgcc cgtacccgca aagaaaacga ccttggcgtg
     2881 ggttattatc agccataatt ctcaggcccg ccgttccggt gggccttgtt ttttctctct
     2941 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
     3001 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
     3061 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
     3121 cgtttgatga caacatcaaa cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
     3181 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
     3241 gtagcctgta tgtcgataca gccccgatgc gcggtcagat tcaggatatc gaaccggaac
     3301 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
     3361 agtacggcaa agcggtcaat atcaaaacca aacgcggcgt aatcaagccg cgcacgccaa
     3421 accaggcgca gtacattgcc aatattctcg accatgacat caccttcggc gttggcccgg
     3481 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
     3541 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
     3601 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
     3661 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
     3721 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
     3781 gccagaacac caccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
     3841 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatacc aagtcaggct
     3901 tgcgtcacgc catcgaagtg ctggctgacg tcgaagagat cagctttaac ttcttccaca
     3961 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
     4021 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
     4081 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
     4141 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
     4201 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
     4261 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggaat
     4321 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagcaca
     4381 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
     4441 tttgttaggt tacgatcaca tcgaagatga cgaagcagaa gaaatggaag ccctcgaaac
     4501 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
     4561 caggcatttg actggcagat gtccccgccg ttggtgatta acgacggcgt tttgattaac
     4621 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
     4681 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
     4741 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
     4801 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt ccgcgacatc
     4861 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
     4921 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
     4981 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
     5041 ttcagcatgg acaaagtgtt acgtcaggca gttgtcgttc ctgaaagtaa gcgcgtagac
     5101 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgtcat tgacgaattc
     5161 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
     5221 gaagacgagt atgacgaaga agatgatatc gactttcgtc agctgagtcg ccatacctgg
     5281 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
     5341 gatgaagagg ttgacactat cggtggtctg gtgatgcagg catttgggca tcttccggcg
     5401 cgtggcgaaa ccatcgacat cgacggttac cagttcaaag tggcgatggc cgacagtcgg
     5461 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccgaa gctggatgaa
     5521 taaaaccgaa actggataga taactacatg gcttttgcct cattaattga acgccagcgc
     5581 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
     5641 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
     5701 cgtccactcc agtctgccgc tattggcttt tgctggggat ttggtctctt tggcagcggt
     5761 attaactggg tctatgtcag catcgcgacc tttggtggaa tgcctggccc ggttaacgtc
     5821 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
     5881 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccgc ccctgctctc
     5941 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
     6001 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgataatggg cgtggaagcc
     6061 attaacttcc tgctgatgat ggttagcggc ctgctggcac tggcgttggt caaacgcaac
     6121 tggcgtccgc tggcggtggc cgtcgtgctg tttgcccttc ccttcccgct gcgttacatc
     6181 caatggttta ccccgcaacc ggaaaaaact attcaggttt cgatggttca gggcgatatt
     6241 ccgcaatcgc tgaaatggga cggagaccag ctacttaata cgctgaagat ttactacaac
     6301 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
     6361 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgcgataaa
     6421 gggagctcgc tggtaaccgg gattgttgac gcgcgtctca ataagcagaa ccgctacgat
     6481 acttacaaca ccatcatcac gctggggaaa ggtgcgccgt acagctacga atcagccgat
     6541 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
     6601 cgtccgttag cgccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
     6661 cagccaccgc tgtcggtaaa cggtattgag cttactgcgg ctatttgcta cgagatcatt
     6721 ctcggcgagc aagtgcgtga taacttccgc ccggataccg attatctgct gactatctcc
     6781 aacgatgcat ggtttggtaa gtctattggt ccatggcagc acttccagat ggcgcgaatg
     6841 cgtgcgctgg agttggcgcg cccgctactg cgtagcacca acaacggcat tacggcggtg
     6901 attggtccgc agggtgagat tcaggcgatg atcccgcagt tcacccgcga agtgttgacc
     6961 actaatgtga ctccgaccac cggactcaca ccgtacgcac gtaccggcaa ctggccgctg
     7021 tgggtactga cggcattgtt tggttttgct gctgtgctga tgagcctgcg tcaacgacgt
     7081 aaataatccc ttcttatcgt gccggaatac atcccgtatt tcggcacgct caccggatgc
     7141 ggcatcagtg cgttatccgg ataacaaaat tcccctatcc aactttggca catctattgc
     7201 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
     7261 ccacgggtag caccaaaaga gtgcatttta agaattttgc ttcggaatgg tgcggcgcat
     7321 ctcgcaaaaa caaacaataa aacagcaata tctttacatt taaccaaact aaatgttacc
     7381 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaaca
     7441 ggtatccatg cgttcttaac gcagaagata aaggagttgg atatgcaatt acgtaaacct
     7501 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
     7561 ccggcagcgg gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
     7621 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
     7681 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
     7741 caggtgaaac tgattccgat cacctcacaa aaccgtattc cactgctgca aaacggcact
     7801 ttcgattttg aatgtggttc caccaccaat aacgtcgaac gccaaaaaca ggcggctttc
     7861 tctgacacta ttttcgtggt cggtacgcgc ctgctgacca aaaagggcgg cgatatcaaa
     7921 gattttgccg acctgaaaga caaagccgta gtcgtcactt ccggcactac ctctgaagtt
     7981 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
     8041 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgat
     8101 gccctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga aatcgtcggc
     8161 aagccgcagt ctcaggaagc ctacggttgt atgttgcgta aagatgatcc gcagttcaaa
     8221 aagctgatgg atgacaccat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
     8281 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
     8341 gacgaaatga aagcactgtt caaagaaccg aatgacaaag cactgaacta attacaagaa
     8401 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
     8461 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
     8521 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
     8581 gggtattttt ttacaacaag ccccgttcgg caacaccacc tatcttggtt ggatctggag
     8641 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
     8701 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
     8761 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
     8821 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
     8881 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
     8941 cgaacaggta cgcgccgcga tccagtcgct gccgcgaggg caaaaaaatg ccgcgctggc
     9001 gatgggcctg acgctaccgc aggcttatcg atacgtactg ttgcctaatg cttatcgcgt
     9061 tatcgtcccg ccgatgacct ccgagatgat gaacctggtg aaaaactctg ccatcgcctc
     9121 gactattggt ctggtggata tggcggcgca ggcgggtaaa ttgcttgatt actcagccca
     9181 cgcctgggag tcgtttaccg ccatcacgct ggcgtatgta ttgattaacg cttttatcat
     9241 gctggtgatg acgctggttg aacgtaaagt tcgcctgcct ggcaacatgg ggggcaaata
     9301 atgtacgagt ttgactggag ttccattgtc ccttccctgc catatctgct cgacgggctg
     9361 gtgatcacgc tgaaaatcac cgtcacagca gtcgtgattg gtattttgtg gggcaccatg
     9421 ctggcggtga tgcgcttgtc cagctttgcg cccgtagcgt ggtttgccaa agcctacgtt
     9481 aacgtattcc gctcgattcc tttagttatg gttttgctgt ggttttacct gatcgtgccg
     9541 ggttttctgc aaaacgtact gggattatca ccaaaaaatg acatccgttt aatttcggcg
     9601 atggtggcgt tttcgatgtt tgaagcagcc tactattcag agattatccg tgccggtatt
     9661 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
     9721 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
     9781 cagggcatcg tactgttcca ggatacctca ctggtgtatg tgttatctct ggccgatttc
     9841 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
     9901 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
     9961 agaaggacag cttaatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    10021 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    10081 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaaccg gtgcagcaag
    10141 gtgaaattac cgtcgatggc atcgtggtta acgataagaa aaccgatctg gcaaagctgc
    10201 gttcccgcgt cgggatggtg ttccagcatt tcgagttgtt cccccatttg tcgattattg
    10261 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcaccc gctcgtgaaa
    10321 aagcattgaa attgctggag cgcgtcgggc tttccgctca tgccaataag tttccggcgc
    10381 agctttccgg cggtcagcag cagcgtgtgg cgatcgctcg cgcattgtgc atggatccta
    10441 tcgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    10501 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    10561 tgggctttgc ccgtaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    10621 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    10681 tcgcgaaaat cctgcattaa tcactctggc gcgcataccc aagtgcgcgc catctcacgc
    10741 tttatcgttt cactcctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    10801 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgacg
    10861 acgctatcgc aatagttctt gccctcgcct caccagagct tgatgtcaaa gcaattacgt
    10921 cttccgccgg aaaccagact ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    10981 tgcttaatcg caccgatatt ccggtagcag gcggcgcggt aaaaccgtta atgcgtgagt
    11041 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    11101 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgtgaaa
    11161 gtgcggaacc tgtcacgatt gtgtctaccg gaccgcaaac taacgttgcc ttgctgctca
    11221 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    11281 ggcttggtaa ctggacgcca gccgccgagt ttaatattta tgttgacccg gaagcggcag
    11341 aaattgtttt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcacaaag
    11401 cacaaattca cgttgaggac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    11461 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    11521 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttaaacccg gagttgttta
    11581 ccactgttga gcgctgggtt ggcgtggaaa cacaagggaa atatacccag ggtatgacgg
    11641 ttgttgatta ttattatctg accggcaata aaccgaatgc tactgtaatg gtcgatgttg
    11701 atcgtcaggg ccttgttgat ttactggctg atcgtctgaa attttacgct taatcactcg
    11761 ccggatgaca tcacgccatc cggcaccact catttcatta aggctcgaac gggcgtctct
    11821 ggaactggtc atgaccacat ttcgggcata gcgtcagcac ttccggtgtg tagatcggga
    11881 gatggaaatg acatttctcg cagaccagat tacccagccc taccacttct ccgctgtgat
    11941 aaaccccgtg atgattgagg tcctggaaaa cttcgcgcca ttcaagctgc gttttatcgg
    12001 tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag acgctgtcag
    12061 actcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca cgtctgacag
    12121 ctcgcgtcag ctcatcgact tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc
    12181 gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt tcgctcagtg
    12241 acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct cccgggtcgt
    12301 taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc ccacaaactc
    12361 tgtaaattaa tgtatgaata atccgggcat catttcaacg tcgaatgcgc gaaaggctgt
    12421 tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca acgctacatt
    12481 tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc gcccggaaga
    12541 gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag taaccgaaga
    12601 cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg gtcgactaca
    12661 catgggccac gtacgtaact acaccattgg tgacgtgatc gcccgctacc agcgtatgct
    12721 gggcaaaaac gtcctgcagc cgatcggctg ggacgcgttt ggtctgcctg cggaaggcgc
    12781 ggcggtgaaa aacaacaccg caccggcacc gtggacgtac gacaacatcg cgtacatgaa
    12841 aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg caacctgtac
    12901 accggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa aaggcctggt
    12961 atataagaag acttctgcag tcaactggtg cccgaacgac cagaccgtac tggcgaacga
    13021 acaagttatc gacggctgct gctggcgctg cgataccaaa gttgagcgta aagagatccc
    13081 gcaatggttt atcaaaatca ccgcttacgc tgacgagctg cttaacgatc tggataaact
    13141 ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc gttccgaagg
    13201 cgtggaaatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt acactacccg
    13261 cccggacacc tttatgggtt gtacctacct ggcggtagcg gcaggtcatc cgctggcgca
    13321 gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc gtaacaccaa
    13381 agttgccgaa gctgaaatgg caacgatgga gaaaaaaggc gtcgatactg gctttaaagc
    13441 ggttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg tattgatgga
    13501 gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact acgagtttgc
    13561 ctctaaatac ggcctgaata tcaaaccggt tatcctggca gctgacggct ctgagccaga
    13621 tctttctcag caagccctga ctgaaaaagg cgttctgttc aactctggcg agttcaacgg
    13681 tcttgaccat gaagcggcct tcaacgctat cgccgataaa ctgactgcga tgggcgttgg
    13741 cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tcccgtcaac gttactgggg
    13801 cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccgaccc cggacgacca
    13861 gctgccggtg atcctgccgg aagatgtggt gatggacggt attaccagcc cgattaaagc
    13921 agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg aaaccgacac
    13981 tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc cggagtacaa
    14041 agaaggtatg ctggattcca aagcggctaa ctactggctg ccggtggata tctacattgg
    14101 tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca aactgatgcg
    14161 tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttactgtgtc agggtatggt
    14221 gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg tttccccggt
    14281 tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg
    14341 ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca acggtatcga
    14401 cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc
    14461 ttctccggct gatatgactc tcgaatggca ggaatccggc gtggaagggg ctaaccgctt
    14521 cctgaaacgt gtctggaaac tggtttacga gcacacagcg aaaggtgatg ttgcggcact
    14581 gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc ataaaacgat
    14641 cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat
    14701 tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg accgcgccct
    14761 gatgcaggaa gcgctgctgg cggtagtccg tatgcttaac ccgttcaccc cgcacatctg
    14821 cttcacgctg tggcaggaac tgaaaggcga aggcgatatc gacaacgcac cgtggccggt
    14881 tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg ttaacggtaa
    14941 agtccgtgca aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc
    15001 tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta aagtgattta
    15061 cgtacccggt aaactcctca atctggtcgt tggctaagcg cgggaggaag cgtgcgatat
    15121 ctggcaacat tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg
    15181 cgtgatacca cgcaggttcc ttccactatg aaggtcatga tcctggactc aggcgatccg
    15241 aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt
    15301 gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg
    15361 aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat gatcatgacg
    15421 gtcaatgcga ccgtgttgat ccccggccgt gacatctacc cgattagcgc caaagtcttc
    15481 cgttcgttct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca agacatgatc
    15541 gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc aagcatccgt
    15601 gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac tccggcaacg
    15661 cctgcacgcg tctctaccac gctgggtaac tgatgattcg gttgtacccg gaacaactcc
    15721 gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt
    15781 tattgcagga aagccaggac gctattcgtc aggtagctgc ggtacaagga ttcgaagaac
    15841 accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg ttatgccagg
    15901 ccatgagtct gtttgccagt cgacaaacgc tattgctgtt gttatcagaa aacggaccga
    15961 atgcggcgat taatgaacaa cttctcacac tcaccggact tctgcatgac gacctgctgt
    16021 tgatcgtccg cggcaataaa ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc
    16081 ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag cttccccgtt
    16141 gggttgctgc gcgtgcaaaa cagctcaact tagaactgga tgacgcggcg aatcaggtgc
    16201 tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag cgtttatcgc
    16261 tactctggcc agacggcaaa ctgacattac ctcgcgttga acaggcggtg aatgatgccg
    16321 cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt
    16381 tgcatattct tcagcaactg cgtctggaag gcagcgaacc ggttattttg ttgcgcacat
    16441 tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat acgccactgc
    16501 gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg ggcgaggcgt
    16561 taaatcgctt aagccagccg cagttacgtc aggccgtgca actcctgaca cgaacggaac
    16621 tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc
    16681 tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa atctttacag
    16741 gctctgtttg gtggcacctt tgatccggtg cactatggtc atcttaaacc cgtggaaacg
    16801 ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt tcctccgcat
    16861 cgtccccagc cggaagcgaa cagcgtacag cgtaaacaca tgcttgaact ggcgattgcc
    16921 gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc ctcttacact
    16981 gcgcaaacac tgaaagagtg gcggcaggaa caagggccgg acgtgccgct ggcgtttatt
    17041 ataggtcagg attcactgct gacctttccg acctggtacg aatacgaaac gatactcgac
    17101 aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc gcaaccgcaa
    17161 taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca ccttcagcct
    17221 gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac cattatccgc
    17281 gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt actgacttac
    17341 attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg atgcgactgt
    17401 ggttaattcg tcatggtgaa acgcaagcga atatcgatgg tctgtacagc ggtcatgctc
    17461 ccacacccct gactgcgcgc ggtattgagc aagcgcaaaa tctgcatacg ctgctacatg
    17521 gtgtttcctt tgacctggtt ttatgcagtg aactggaacg tgcacagcat accgcgcgac
    17581 gggttctcag tgatcgccag cttcccgtgc aaatcatccc tgaactcaac gaaatgtttt
    17641 ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc gaaaactata
    17701 gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga tttcaggcat
    17761 tttcacaacg agtggaacgg tttatcgcaa ggcttagtga atttcagcac tatcagaata
    17821 ttttagtcgt cagccatcag ggtgtactga gtctgttaat cgcccgttta attggtatgc
    17881 ctgccgaagc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc attgatatca
    17941 accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc gagaatgcat
    18001 gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg ggcaggctga
    18061 tattctccgc agccagactt ttttcgccag acacgacttt gtagaaattg ttttacaaaa
    18121 atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa gccgattcgg
    18181 cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt tttatctatt
    18241 tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt tatcgacaaa
    18301 attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcagggcaa atccagcatc
    18361 accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc cattgctgac
    18421 cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac
    18481 agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat gcaggaagag
    18541 agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc
    18601 gccgtgggaa cgaaaatgcc ggactgggta caaactggtt ttaccgagta cctgcgtcgt
    18661 tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggaaaacg cggcaagaat
    18721 gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa
    18781 aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca gttagccgct
    18841 gagctggaac gctggaagct ggatggtcgc gacgtcagtc tactgattgg cgggcctgaa
    18901 gggttgtcgc ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc ggcgcttacc
    18961 ctcccccatc cgttggttcg cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc
    19021 accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa aacgcagcgg
    19081 atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg
    19141 gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc caacctgtat
    19201 aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa ccgcattaag
    19261 ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat ccctctggcc
    19321 ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa cgtgcagcaa
    19381 acgctggacg ctttgcgcag cgtggttgat ctgaccgatg acgatattgc tgcattccga
    19441 aaagagcgcg cacgttcaca ccgtttcacc tccattccgg tgaaaactaa cctgaccgaa
    19501 gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgttga agttaaaggc
    19561 tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg
    19621 aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc caactatgcg
    19681 gcaacgcatg atatcggtaa gctgggcatt gagcgttact atgaagacgt gctgcacggt
    19741 cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa
    19801 gaagtgccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa actccagcaa
    19861 tatattgaaa cgctgctggc gggtagccgc gcagcggtgg tagtcaccga tccgcgtaca
    19921 ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt tgttgacggt
    19981 atctccagca aagattattc cgccttgctg aacgacccga atacaccgct ggtgaaccgc
    20041 gctacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc ggtttcggca
    20101 ttgagcgccg gagtgatcac gcgcaatacg acgctgtttg acccaggctg gtggcaactg
    20161 ccaggttcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc
    20221 acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta cgatatggga
    20281 atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac cggtatcgac
    20341 ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa acgctttaaa
    20401 aaaccgtggt atcagggtga caccattccg gttggtatcg gtcagggtta ctggacagcg
    20461 accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat cgtgaaggtt
    20521 ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt acaaccgcat
    20581 gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga cggtatgtac
    20641 ggtgttgcta accgccctaa cggtacggcg cataaatact ttgccagcgc accgtacaaa
    20701 attgcggcga aatccggtac cgctcaggtc ttcggtctga aagcgaacga aacctataat
    20761 gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt tgcgccatac
    20821 aacaatccgc aagtcgctgt cgccatgatt ctggagaacg gcggtgcggg tccggcggtt
    20881 ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa caccgatctg
    20941 cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg gataatccga
    21001 ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg atcttactgg
    21061 cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat attggcatga
    21121 tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg atggcgcaaa
    21181 ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt attattttgc
    21241 tggtggcggt agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg ctggacctcg
    21301 gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg atggttgcgc
    21361 gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa cacaggcatc gcgctagtgc
    21421 tgatatttat gcccacgctg ctggtggctg cacagcctga cctggggaca tcaatcctcg
    21481 ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt ctgattggcg
    21541 tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg atgcatgatt
    21601 accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc ggcgcgggct
    21661 atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc aaaggctggc
    21721 tgcatggcac tcagtcacaa cttgaatttc tccccgaacg ccataccgac tttatcttcg
    21781 cggtactggc ggaagagctg ggattagtgg gcattttgat tctgctcgct ctctacattc
    21841 tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt ggtcgcgtca
    21901 tggctggcgg cttaatgctg atattattcg tttatgtctt cgtaaatatt ggtatggtaa
    21961 gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga ggatcggcgc
    22021 taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac aggaaaatgt
    22081 tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg
    22141 gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt aagtgtaccg
    22201 cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc gcgtttcgaa
    22261 ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg
    22321 caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc cgaacccggc
    22381 agtaacctga cggcctctgg cgaagcattc gatccgacac agctgacagc ggcccatcca
    22441 acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg aatgatcgtg
    22501 gtgcgcatta atgatcgcgg tccttacggc aacgaccgcg ttatttcgct ttctcgcgcg
    22561 gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc gattattgtc
    22621 gcccaggatg gttcgctttc tggtcctggt atggcctgta ccacagttgc caaacagact
    22681 tacgccctgc ctgcacctcc cgatttaagc ggcggcgcgg gaacaaattc agtgtctggc
    22741 ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc
    22801 gcgccggtaa ccagcagcgg tttcctcggc gcaccaacga ccttagcgcc tggtgttctg
    22861 gaaggcagcg aaccaacacc tgcaccacag cccgttgtta cagctccggc gacaacgcct
    22921 gcaaccacgc ctgcaatggt gacaccgcaa gccacctcgc aaagcgccag cggcaacttt
    22981 atggtgcaag tcggagccgt tagcgatcag gctcgtgcgc aacagtacca acagcaactg
    23041 ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcagtctg gcggatccag
    23101 cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa
    23161 gcccaattac agtcatttat taccaccgcg cagtagcgta aagcaggcat ctgatgtgtc
    23221 aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc cggtagtgca
    23281 tttgctatag tagggcactt tttttaattc catcacggat gtcgtagttc agaccatgaa
    23341 taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc tttgcacagc
    23401 ctttatctct gctgcacatg ccgatgacct gaatattaaa actatgatcc cgggtgtacc
    23461 gcagatcgat gcggagtcct acatcctgat tgactataac tccggcaaag tgctcgccga
    23521 acagaacgca gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga ccagttacgt
    23581 tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca ccatcggtaa
    23641 cgacgcatgg gctaccggta acccggtgtt taaaggttct tcgctgatgt tcctcaaacc
    23701 gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat cgggtaacga
    23761 tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg ttggcttgat
    23821 gaacagctac gttaacgcac tgggactgaa aaatacccac ttccagacgg tacatggtct
    23881 ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc aggcgttgat
    23941 ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt ttaacggtat
    24001 tcgccagctg aaccgtaacg gtctgttatg ggataacagc ctgaatgtcg acggcatcaa
    24061 aaccggacac actgacaaag caggttacaa ccttgttgct tctgcgactg aaggccagat
    24121 gcgcttgatc tctgcggtga tgggcggacg tacttttaaa ggccgtgaag ccgaaagtaa
    24181 aaaactgctg acctggggct tccgtttctt cgaaaccgtt aacccactga aagtaggtaa
    24241 agagttcgcc tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt taggtgttga
    24301 taaagacgtg tatctgacca ttccgcgtgg ccgcatgaaa gatctgaaag ccagctatgt
    24361 gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg gtactatcaa
    24421 cttccagctt gatggcaaaa cgatcgaaca acgcccgctg gttgtgctgc aagaaatccc
    24481 ggaaggcaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc atcactggtt
    24541 tggttaaaaa tcgaacact
//
LOCUS       DAOTRK010000066        24468 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.066, whole genome shotgun sequence.
ACCESSION   DAOTRK010000066 DAOTRK010000000
VERSION     DAOTRK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.066"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(343..1002)
                     /gene="rluA"
                     /locus_tag="QIE41_002534"
     CDS             complement(343..1002)
                     /gene="rluA"
                     /locus_tag="QIE41_002534"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDS3531411.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1014..3920)
                     /gene="rapA"
                     /locus_tag="QIE41_002535"
     CDS             complement(1014..3920)
                     /gene="rapA"
                     /locus_tag="QIE41_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDS3531412.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAKSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4084..6435)
                     /gene="polB"
                     /locus_tag="QIE41_002536"
     CDS             complement(4084..6435)
                     /gene="polB"
                     /locus_tag="QIE41_002536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDS3531413.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREAGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGTIVNARLKPHPDYRPPLRWVSIDIETT
                     RHGELYCIGLESCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6510..7205)
                     /gene="araD"
                     /locus_tag="QIE41_002537"
     CDS             complement(6510..7205)
                     /gene="araD"
                     /locus_tag="QIE41_002537"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDS3531414.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGKVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(7374..8876)
                     /gene="araA"
                     /locus_tag="QIE41_002538"
     CDS             complement(7374..8876)
                     /gene="araA"
                     /locus_tag="QIE41_002538"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDS3531415.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESSYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(8887..10587)
                     /gene="araB"
                     /locus_tag="QIE41_002539"
     CDS             complement(8887..10587)
                     /gene="araB"
                     /locus_tag="QIE41_002539"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDS3531416.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAQNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            10926..11804
                     /gene="araC"
                     /locus_tag="QIE41_002540"
     CDS             10926..11804
                     /gene="araC"
                     /locus_tag="QIE41_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDS3531417.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            11890..12654
                     /locus_tag="QIE41_002541"
     CDS             11890..12654
                     /locus_tag="QIE41_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDS3531418.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATVVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAVGVVALVVLIRHPLMPMYIDILRKVVGG"
     gene            complement(12768..13466)
                     /gene="thiQ"
                     /locus_tag="QIE41_002542"
     CDS             complement(12768..13466)
                     /gene="thiQ"
                     /locus_tag="QIE41_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDS3531419.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NTAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASALLGITG"
     gene            complement(13450..15060)
                     /gene="thiP"
                     /locus_tag="QIE41_002543"
     CDS             complement(13450..15060)
                     /gene="thiP"
                     /locus_tag="QIE41_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDS3531420.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRVRQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(15036..16019)
                     /gene="thiB"
                     /locus_tag="QIE41_002544"
     CDS             complement(15036..16019)
                     /gene="thiB"
                     /locus_tag="QIE41_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDS3531421.1"
                     /translation="MLKKCLPLLLLCTAPVFAKSVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYATANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSH"
     regulatory      complement(16050..16149)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16391..16960
                     /locus_tag="QIE41_002545"
     CDS             16391..16960
                     /locus_tag="QIE41_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDS3531422.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTHSQIIRMVNLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNCDDYSKYFTGAVDGDARDKFTHWLIN
                     FNKLKEHAIDPENMAAKASLSSGDTLRVSFFIGDEAILTLDLQLKESTSTGCIDLSHA
                     YFNGVVICGIDRLEVDLSNAETNNSRWYD"
     gene            complement(17190..18848)
                     /gene="sgrR"
                     /locus_tag="QIE41_002546"
     CDS             complement(17190..18848)
                     /gene="sgrR"
                     /locus_tag="QIE41_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDS3531423.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     EIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETISNFASHPIGTGPYGVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLESLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            18937..19068
                     /gene="sgrT"
                     /locus_tag="QIE41_002547"
     CDS             18937..19068
                     /gene="sgrT"
                     /locus_tag="QIE41_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDS3531424.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSGY"
     gene            complement(19176..19781)
                     /gene="leuD"
                     /locus_tag="QIE41_002548"
     CDS             complement(19176..19781)
                     /gene="leuD"
                     /locus_tag="QIE41_002548"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDS3531425.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIYFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPEFMR"
     gene            complement(19792..21192)
                     /gene="leuC"
                     /locus_tag="QIE41_002549"
     CDS             complement(19792..21192)
                     /gene="leuC"
                     /locus_tag="QIE41_002549"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDS3531426.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDNAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21195..22286)
                     /gene="leuB"
                     /locus_tag="QIE41_002550"
     CDS             complement(21195..22286)
                     /gene="leuB"
                     /locus_tag="QIE41_002550"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDS3531427.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(22286..23857)
                     /gene="leuA"
                     /locus_tag="QIE41_002551"
     CDS             complement(22286..23857)
                     /gene="leuA"
                     /locus_tag="QIE41_002551"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDS3531428.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(23950..24036)
                     /gene="leuL"
                     /locus_tag="QIE41_002552"
     CDS             complement(23950..24036)
                     /gene="leuL"
                     /locus_tag="QIE41_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDS3531429.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         5566 a         6578 c         6557 g         5767 t
ORIGIN      
        1 agaaagacct gcatgatgag ctagagaaga ggctggtgat gcaaggcgtc gtgcacgacg
       61 cggatcaccg agatgggcat cgccaaccag actgctaatt agcccatgaa taacaatcag
      121 aaaggaccat aacagacccg ataaaatgaa ataatagaga cgatcaacgg gtgaagaaaa
      181 agttcaaaaa ttcgctgtgg agcaggaagg gaattaccga atggaaagcg tagccacacg
      241 caacaactga aagcagtttg gcagaaacaa aaaatccccg gactcgggga tttatgtaca
      301 agaggcagcc cttaggatga gggtataaac gtacaggaaa ggttaaaaat ccgctggcgc
      361 tttaaacgtc atactattgc catacgccgg atgggtaatc gtcagcatct ctgcatgtag
      421 caacaaacgc ggtgccattg ctctcgcttc tggtgatgca taaaaacgat cgccgagaat
      481 cggatgaccc agcgccagca tatgcacacg cagctgatgc gaacgcccgg taatcggttt
      541 taacaccact cttgccgtgt tatccgccgc atattccacc acttcatact ccgtctgcgc
      601 aggtttacct gtttcgtaac agactttctg tttcgggcgg tttggccagt cgcaaatcag
      661 cggcagatcc accagacctt ctgcggggga tggatgcccc cagacgcggg ccacatactg
      721 ctttttcggc tcgcgctcgc ggaactggcg ttttaactcc cgctccgcgg ctttggtcag
      781 cgccactaca atcacgccgc tggtagccat atccagacga tgcaccgatt ctgcctgcgg
      841 ataatcacgc tgaatgcgcg tcatcacgct gtctttgtgc tcttccagac gacccggcac
      901 agacaacaaa ccgctcggct tgttgaccac cataatatgg tcatcctgat acaggataac
      961 caaccagggt tcctgcggtg gattgtagtt ttccatcccc attttcggct ccgttactga
     1021 tgcgttacaa cgatcaaacg cagggcatcc agacgccagc ctgcctgatc caggctttcc
     1081 attacctgct gacggttgct ctcaatggcg gtcagttcgt cgtcacgaat gttcgggttc
     1141 actgcacgca gagcttccag acgagacagc tcggcagaca gtttttcgtc ggcttcgtta
     1201 cgtgcggcat caatcagtgc acgggcagat ttctcgatct gtgcttcccc cagctgaagg
     1261 atagcgtgaa catcctgctg cacggcgtta accagtttgc tgccggtgtg acggttaacc
     1321 gcgttaagct gacggttaaa ggtttcaaac tccacctgcg ccgccaggtt gttgccgttt
     1381 ttatccagca gcatacgtac cggcgtcggt ggcaggaagc ggttgagctg caactgcttc
     1441 ggagcctggg cttcgaccac gtaaatcagt tccaccaaca gcgtacctac cggcaacgct
     1501 ttgtttttca acagtgaaat cgtgctgcta ccggtatcgc cagaaaggat cagatccaga
     1561 ccgttgcgga tcagcggatg ctcccaggta ataaactgcg catcttcacg cgccagcgcc
     1621 acttcacggt cgaaggtgat ggtgatgcca tcttccgaca agcctgggaa gtccggcacc
     1681 agcatatgat cggacggggt cagcacgatc atgttgtcgc cgcgatcgtc ctgattgata
     1741 ccgataatat cgaacagatt catggcgaag gcgatcaggt tggtatcgtc atcctgctct
     1801 tcaatgcttt ctgccagtgc ctgggctttt tcgccaccgt tggaatggat ttccagcagg
     1861 cggtcacgac cctgttccag ctgtgctttc agcgcttcat gttgctcgcg gcagtttttg
     1921 atcagatcgt caaagccttc ggtttgatcc ggtgaagcca gatagttaat cagatcgttg
     1981 tatacactat cgtaaatagt gcgtccggtc gggcaggtgt gctcgaatgc atccagacct
     2041 tcgtgatacc agcgcaccag caccgactga gcggttttct ccagataagg cacatggatc
     2101 tgaatatcgt gcgcctggcc gatacgatcc agacgaccaa tacgctgctc cagcagatcc
     2161 gggttgaatg gcaggtcaaa catcaccatg tggctggcga actggaagtt acgtccttca
     2221 gaaccgattt ccgagcacag cagtacctgt gcgccggtgt cttcttcagc aaaccaggcg
     2281 gcagcgcggt cacgctcaat aatcgacatg ccttcgtgga acaccgcagc gcggatacct
     2341 tcacgttcgc gcagtacttg ctccagttgc agcgcagtgg cagctttggc gcagatcacc
     2401 agcactttct gagagcgatg gctggtcagg tagcccatca gccactcaac gcgcggatcg
     2461 aagttccacc aggtggcgtt atcaccttca aattcctgat aaatacgctc cgggtagagc
     2521 atatcgcgag cgcgatcttc cgcacttttg cgtgcgccca taatgccgga gactttaata
     2581 gccgtctgat actgcgtcgg cagcggcagc ttgatggtgt gcagctcgcg tttcgggaag
     2641 cctttcacac cgttacgcgt gttacggaac agcacgcggc tggtgccgtg gcgatccatc
     2701 agcatggaaa ccagttcctg acgggcgctc tttgcatctt cgctgtcgct gtttgctgct
     2761 tgcaacagcg gctcgatatc ctgctcgccg atcatctcac cgagcatgtt cagttcatca
     2821 ttgctcagtt tgttacctgc cagcagcatg gcaacggcgt ccgcaaccgg acgataattt
     2881 ttctgttctt caacgaattg cgcaaaatcg tggaaacggt tcgggtccag cagacgcaga
     2941 cgggcgaagt ggctttccat ccccagttgt tccggggttg cggtcagcag cagaacgccg
     3001 ggcacgtgct ctgccagttg ttcaatggcc tgatattcgc ggctcggcgc atcttcgctc
     3061 cacaccaggt gatgggcttc atcgaccacc agcagatccc attcggcttc acagagatgt
     3121 tccagacgct gtttgctacg acgggcaaaa tccagcgagc aaatcaccag ctgttcggtg
     3181 tcgaacgggt tgtaagcatc gtgctgagct tcggcataac gctcatcatc aaacagagca
     3241 aagcgcaggt tgaaacggcg cagcatttct accagccact gatgctgtaa ggtttccggg
     3301 acgataatca gcacacgttc agcagcgcca gagagcagtt gctgatgcag gatcatcccg
     3361 gcttcaatgg ttttccctaa accgacttcg tcagcgagca ggacgcgcgg cgcgtggcgg
     3421 cgaccaacat catgagcgat gttgagctgg tgcgggatca ggctggtacg ctgaccgcgc
     3481 aggccgctgt acggcatacg gaactgttcg ctggaatact tacgcgcgcg ataacgcagc
     3541 gcaaagcggt ccatacggtc aatctgcccg gcaaacagac ggtcttgcgg tttgctgaac
     3601 accagtttgc tatcaaggaa aacttcacgc agggctacgc cggactcttc agtatccagg
     3661 cgagtaccga tataggtcag caagccattt tcttctttta cttcttcgac ttgcatctgc
     3721 cagccgtcat ggctggtaat agtatcacca gggttgaaca tcacgcgggt cacgggggaa
     3781 tcactgcgtg cgtacagacg gttttcacca gtagatggga aaagtaaagt gacagttcgc
     3841 gcatccaccg cgacaacggt tccaagtccc aattcgcttt ctgtatcgct gatccagcgt
     3901 tgaccaagtg taaaaggcat atgtgttcgg ctctatatct ttaattgcag gcaataacca
     3961 cccgctaccg tgcttatgag gtagtggtgt tattcaggtc caggaatgga aagggcgcta
     4021 tggtactgga tggcaaagca ttcgtcacgc atcaaaatgg tatctggcga actctttttt
     4081 tgctcaaaat agcccaagtt gcccggtcat aagtgtagca aaattatcct caataaaagg
     4141 gagtattccc tccgccacgg gttgtagctg gcgggtcaga tagtgttcgt aatccagcgg
     4201 tgaacgttgg tagtccagcg gctccgggcc gttggtggtc catacgtact taatggtgcc
     4261 gcggttctga tattgcaagg ggcgaccgcg cttttggttt tcttcatcgg caaggcgagc
     4321 ggcgcgtaca tgaggcggaa cattacgctg atattcgctc agcggacggc gaaggcgttt
     4381 acggtaaacc agccgcgcat ccagttcacc cgccatcagt ttgtcgatgg tttcgcggac
     4441 gtactcctga tatggctcgt tgcggaagat acgcaggtac agctcctgct gaaactgctg
     4501 ggccagcggc gtccagtcgg tgcgcacggt ttccagccct ttaaacacca tccgctgttt
     4561 atcgccctcc tgaatcagcc cggcataacg ctttttacta ccggtatcgg ctccgcgaat
     4621 ggttggcatc agaaaacggc agaaatgggt ttcatactcc agttctaatg cgctggtcag
     4681 ccgttgtttt tgcagcgttt ctgcccacca ggcgttaacg tgctgcacca gtgcacgacc
     4741 aattttcgcc gcttcttctt ccgaatgtgc gcctttcaac cagacaaacg ttgagtcggt
     4801 atcgccgtag atcacgtcgt agccctgcgc ttcaatcaat gctttggttt gccgcatgat
     4861 ctgatgacca cgcatggtga tcgacgacgc caggcgcgga tcgaagaagc ggcaggcggt
     4921 ggtgccaagc acgccataaa aggcattcat aatgatcttc agcgcctgcg aaagcggctt
     4981 attaccctga cgtttggctt catcgcgccc gtgccagatg ttagtcacaa tctccggcag
     5041 gcaatgtttc tcccgcgaga accaggcgtc gagaaaacct tcggtgctgt gctctggatc
     5101 aggctgcgcc atgccttcca ccagcccgac gggatcaatc agaaaggtgc ggatgatcga
     5161 cgggtacagg cttttgtagt ccagcaccag taccgaatca taaagccctg gccgcgaatc
     5221 catcacatag ccgccagggc tggcgtgtgg cggcacttcg ccgagattag gcgcgacata
     5281 accagcgcga tgcattcggg gaaaatagag atgaccaaac gctgccaccg accccccgtg
     5341 gcggtctacc ggcaggccgt taaccgtcgc tcgttcgagt aaaaacggca tgatttcagt
     5401 tttgtggaag atctgcgtca ccagttcgca atctttcagg ttataagttg ccagcgcagg
     5461 tttatcttcg gcgaaacggc ggtcaatttc gtccattcga tcccacgggt tatcgataga
     5521 ttttccttcg cctaatagct cctgagcgac agtttccagc gagaatgaag agaaattcca
     5581 gaacgcggat ttcagcgcct cgataccgtc gataatcagt cgccctttgg cttgggcaaa
     5641 aaagacgccg tttttgaagc cgtgttcgcg ccactccagc tcgctgttat cacgcccaag
     5701 acgcagtgga atacgataac gctcggcatg tttttgcagc attcgcagat caaactgcac
     5761 cacgttccag ccgatgatca catcaggatc gtgagtcgca aaccaggcgt tgagtttttc
     5821 cagtagctgc gggcggctgg cgacgtattc cagttcgaaa tcgagcgcgg aggcgtcgcc
     5881 attctctggc cccagcatat aaacgatgcg ctgcccgcaa ctttccaggc cgatgcagta
     5941 cagctcaccg tggcgggtgg tttcaatatc aatagaaacc caccttagcg gcggacgata
     6001 gtcgggatgc ggtttcagac gggcattaac aatagtgcca ttgcgcatat caccctcgac
     6061 ccacaccggt gaggtgataa accgctccat cagatagcgt tctggcgggc gcacatcggc
     6121 ctcgtagacg gtaacgccag cttcacgcag gcgcttttcg taattcatca actggcgatg
     6181 ggcgcgacag taaaggccat acaccggttg gcggtgaaaa tcttttaacg ccagcggcgt
     6241 caagcgaaag ccttgttcac cctgcaaaat atgctgagcg cggggaacct gatcggcggg
     6301 aataaacgcc acggactctt gcggtgcaag cgtaacctgc aacggcccgt tgtccgtcgc
     6361 cagccagaag gagacctctg tcccttgcgg ggtgtcccgc cagtgtcggg ttaagataaa
     6421 acctgcctgc gccacgctaa aaatccaata aaaaaccagg cttgattata gcctggtttc
     6481 gtttgattgg ctgtggtttt atacagtcat tactgcccgt aatatgcctt cgcaccatgc
     6541 ttacgtaaat aatgtttatc cagcaacgtt tgctgcatat ccggtaactg cggagcaagc
     6601 tggcggcaga aaatccccat ataagcgact tcttccagca cgatggcgtt gtgcaccgca
     6661 tcttcggcat ttttgcccca tgcaaacggg ccgtgagaat ggaccagaac gccgggcatt
     6721 tgtgctgcgt cgatcccctg tttttcgaag gtttcgacga tcacttttcc tgtttcccat
     6781 tcatattcgc cgttgatttc cgcatcggtc attttgcggg tgcagggaat ggtgccgtag
     6841 aaatagtcgg cgtgggtggt accggttgcc ggaattgact ggcccgcctg cgcccagatg
     6901 gtggcgtggc gcgagtgtgt gtgcacaatg ccgccaatgg acgggaatgc ctgatagagc
     6961 agccggtgag ttggcgtgtc ggaggagggc tttttcgttc cttcaaccac ttcaccggtt
     7021 tcgatgctga ccacgaccat atcgtcagcg gtcatgacgt tgtaatcgac gccggaaggt
     7081 ttgatcacaa agacgccgcg ctcgcgatca acggcgctga cgttgcccca tgtgagcgtg
     7141 actaggttat gttttggcag cgccaaattg gcttctaata cctggcgttt gagatcttct
     7201 aacatgttga ctcctgagag gtcgtttccc ctcgccccgg ccctctcccc aaaggggcga
     7261 gggggaaaac aatgccggat gcgctttgct tacccggcct acaaaatcgc agcgtgtagg
     7321 cctgataaga cgcgccagcg tcgcatcagg cgttacacac ctgatgcagc tacttagcga
     7381 cgaaatccgt aatacacttc gttccagcgc agcgcgtctt taaacgcggg caggcgggtg
     7441 tcgttatcaa tcaccgtgat ttcaatgtcg tgcatctcgg caaactggcg catatcgttg
     7501 aggttcagcg catggctgaa aacagtatgg tgcgcgccac cagcaaggat ccatgcttcg
     7561 gaagcagttg gcagatccgg ttgcgctttc cacagcgcat tcgccaccgg cagtttcggc
     7621 agggagtgcg gtgttttcac agtgtcgatg cagttaacca gcagacggta acgatcgccg
     7681 agatcaatca ggctggcgac aatcgccgga ccagtttggg tattgaagat caggcgggca
     7741 ggatcgtctt taccgccaat accgaggtgt tgaacgtcga ggatcggttt ctcttctacg
     7801 gcgatcgacg gacagacttc cagcatatgg gagccgagca ccagatcatt acctttctcg
     7861 aagtgatagg tgtagtcctc cataaaggag gtgccgccct gcagaccggt tgacatcacc
     7921 ttcatgatgc gaagcagggc ggcagttttc cagtcgcctt cgcccgcaaa gccgtaaccc
     7981 tgctgcatca gacgctgtac ggccagacca ggaagctgtt tcagaccgtg caaatcttca
     8041 aaggtggtgg tgaacgcgtg gaagccacct tgttccagga aacgcttcat ccccagctca
     8101 atacgcgccg cttccagcac gttctggcgt ttttcgccgt ggatttgtgt ggcaggcgtc
     8161 atggtgtagc tgctttcata ctcatcgacc agcgcgttaa catcgccatc gctgatggag
     8221 ttcaccacct gcaccagatc gccaaccgcc caggtattga cggagaaacc gaacttgatc
     8281 tgtgcggcaa ctttatcacc atcggtgacc gctacttcac gcatgttatc gccaaaacgg
     8341 cagactttca gatgacgggt atcctgttta gataccgcct gacgcatcca ggagccgata
     8401 cgctcatggg cttgtttatc ctgccagtga ccggtaacca cggcatgttg ctgacgcata
     8461 cgcgcgccaa tgaagccgaa ctcgcgaccg ccatgtgcag tctggttcag gttcataaag
     8521 tccatatcga tgctatccca cggcagcgcc gcgttgaact gggtgtggaa ttgcagcaac
     8581 ggtttgttga gcatggtcag gccgttgatc cacattttgg ccggggagaa agtgtgtagc
     8641 cacactacca agccggcgca acgatcgtcg taattcgcgt cacggcaaat agcggtgatt
     8701 tcatccggcg tggtgcccag cggtttcagc accagtttgc agggcagttt cgcttccgta
     8761 ttcagcgcat taacgacgtg ctcggcatgt tgggtgacct gacgcagggt ttccgggcca
     8821 tacagatgct ggctgccaat tacaaaccac acttcataat tatcaaaaat cgtcattgtc
     8881 gtgtccttat agagtcggaa cggcctgggc agcctgtgcc ggggcggaag ttggaagata
     8941 gtgttgttcg gcgctcatcg cccattgctg atagcggcga taaagctgtt caaagcgttg
     9001 tgcctgttcg ctgcgcggtt gcagggtttt ctctaccgca ctggccattt tttgctgggc
     9061 tgaggggatg tctgcgtgca ctttcgcggc gacggcagca aaaatcgccg caccgagcgc
     9121 acagcattgg tcagaggcaa caatttgcag cgggcgattc agcacgtcgc agcaggcctg
     9181 cataatgacc tggtttttcc gcgcgatgcc gcccagcgcc atcacgttat tgacggcgat
     9241 cccctgatcg gtaaagcact ccatgattgc gcgtgcgcca aaggcggtgg cagcaatcaa
     9301 accgccgaac agcagcggag cgtcggtagc gaggttcaga tcggtaatca cccctttcag
     9361 gcgttggtta gcgtttggcg tgcggcggcc gttaaaccag tcgagcacca ccggcaggtg
     9421 atccagagac ggattttgag cccatgcttc ggtcagcgcc ggaagcagtt gtttctggct
     9481 ggcgttgatt tgctctttca gttccggatg ctgggcggca agctgttcca gcggccagcc
     9541 gagtacgcga ccaaaccagg cgtagatatc accaaacgcc gattggcctg cttccagacc
     9601 gataaatcca ggcaccacgc tgccatcaac ctgaccgcaa atacctttca ctgcccgctc
     9661 gccaacgctc tgtttgtcgg caatcagaat gtcgcaggtg gaagtaccga taacttttac
     9721 cagagcgtta ggctgtgcgc ctgcaccaac tgcgcccata tggcagtcaa acgcgccgcc
     9781 ggaaatcacc acgctttcag gcaggccgag acgctgcgcc cattccgggc ataaggtgcc
     9841 caccggaata tcggcagtcc aggtatcagt gaacagcggg gaaggcaaat ggcgattgag
     9901 gatcgggtcc agctcatcaa agaaactggc tggcggcagg ccgccccagc tttcgtgcca
     9961 tagcgactta tgcccggcgc tgcaacgtcc gcgacgaata tcctgcgggc gcgtggtacc
    10021 ggaaagcaga gctggcaccc agtcgcacag ctcaatccac gatgcggcag attgcgccac
    10081 ggcgctgtcc tggcgagtta catgcagtat ttttgcccag aaccattcgc tggaataaat
    10141 accgccaata tagcgggagt agtcaacgtt gcccggcgtg tgacataagc gggtaatctc
    10201 ttccgcttct tcaaccgcgg tgtggtcttt ccacaatacg aacatcgcgt tcgggttttc
    10261 ggcaaactcc gggcgcagcg ccaggacgtt accgtccgcg tcaatcggtg cgggcgtcga
    10321 gccggttgtg tcaacgccaa tcccgaccac agctgcgcgc tgctcggcgc taagctctgc
    10381 aagcacggtt ttcagcgccg cttccattga ctcaatgtag tcacgcggat gatgacggaa
    10441 ctggttattt ggggcatcac aaaattgccc tttctgccaa cggggatacc actctacgct
    10501 ggtggcgatc tcttcaccgg tggcgcagtc caccgccaaa gctcgcacag aatcactgcc
    10561 aaaatcgagg ccaattgcaa tcgccatcgt ttcactccat ccaaaaaaac gggtatggag
    10621 aaacagtaga gagttgcgaa aaaaagcgtc aggtaggatc cgctaatctt atggataaaa
    10681 atgctatggc atagcaaagt gtgacgccgt gcaaataatc aatgtggact tttctgccgt
    10741 gattatagac acttttgtta cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa
    10801 tgcttttaat aagcggggtt accggttgga ttagcgagaa gagccagtaa aagacgcagt
    10861 gacggcaatg tctgatgcaa tatggacaat tggtttcttc tctgaatggc gggagtatga
    10921 aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aacgcccatc
    10981 tggtggcggg tttaacgccg attgaggcca acggttatct cgattttttt atcgaccgac
    11041 cactgggaat gaagggttat attctcaatc tcaccatacg cggccagggg gtggtgaaaa
    11101 atcagggacg agaatttgtc tgccgaccgg gtgatatttt gctgttcccg ccaggagaga
    11161 ttcatcacta cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg gtttactttc
    11221 gtccgcgcgc ctactggcat gaatggctta actggccgtc aatatttgcc aatacgggtt
    11281 tctttcgccc ggatgaagcg caccagccgc atttcagcga cctgtttggg caaatcatta
    11341 acgccgggca aggggaaggg cgctattcgg agctgctggc gataaatctg cttgagcaat
    11401 tgttactgcg gcgcatggaa gcgattaacg agtcgctcca tccaccgatg gataatcggg
    11461 tacgcgaggc ttgtcagtac atcagcgatc acctagcaga cagcaatttt gatatcgcca
    11521 gcgtcgcaca gcatgtttgc ctgtcgccgt cgcgtctgtc gcatctgttc cgccagcagt
    11581 taggaattag cgtcttaagc tggcgcgagg accaacgcat tagtcaggcg aagctgcttt
    11641 tgagcaccac ccggatgcct attgctaccg tcggtcgcaa tgttggtttt gacgatcaac
    11701 tctatttctc gcgagtattt aaaaaatgca ccggggccag tccgagcgag ttccgtgccg
    11761 gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc ataattggta acgaatcaga
    11821 caattgacga cttgacggag tagcataggg tttgcagaat ccctgcttcg tccatttgac
    11881 aggcacatta tgcaagcatt gctggaacac tttattaccc aatccaccgt gtattcattg
    11941 atggcggtgg tgttggtggc ctttctggag tcgctggcgc tggtcggttt gattctaccc
    12001 ggtacggtgc tgatggcggg gctgggggcg ctgattggca gcggcgagtt aagtttctgg
    12061 cacgcctggc tggcagggat cgtcggttgc ctgctgggcg actggatctc cttctggctg
    12121 ggctggcgtt ttaaaaagcc attgcatcgc tggtcatttc tgaagaaaaa caaagcacta
    12181 cttgataaaa ctgaacatgc gttgcatcaa cacagcatgt tcaccattct ggtcggtcgt
    12241 tttgttggcc cgacgcgtcc gctggtgcca atggtggcgg gaatgctgga tctcccggtg
    12301 gcaaaattta ttacgccgaa tattatcggc tgcctgctgt ggccaccgtt ttacttcctg
    12361 ccggggattc tggcgggtgc ggcgatcgat attccagccg gaatgcagag cggcgagttt
    12421 aaatggttgc tgctggcgac ggtggtattt ttgtgggttg gtggctggct gtgctggcgg
    12481 ttatggcgca gcggtaaagc aaccgatcgt ttaagtcatt atttgtcgcg cggtcgtttg
    12541 ttgtggctga cgccgttgat ttctgccgtc ggtgtagtgg cgctggtggt gttaattcgc
    12601 cacccgttga tgccgatgta tatcgatatt ttgcgtaaag tggttggggg ttaggggata
    12661 gccttgtgcg ggttgcctga tgcgacgctt gccgcgtctt atcaggccta caaaacgcac
    12721 tgtccgtagg tcggataagg cgttcacgcc gcatccgaca gtgcatacta acccgtaatc
    12781 cccaatagtg ccgaagcact cgccttaccg ctcaacaact catcggtcat accctgccag
    12841 gcgatgcgcc cgtcggcgac caccaccgag cgtgtggcga tccgcgccgc atcttccaca
    12901 ctgtgcgaca ccatcaataa cgtcattttt tgctgctggc agctcgtgct caccagcgtc
    12961 aacatctcct gacgtaacgc cggatcgagc gcagagaacg gttcatcgag caataaaatc
    13021 ggctgttcgc gcaccagaca acgcgctaac gccacgcgct gtcgttgacc gccagaaagc
    13081 tcgcccggta atcgcgccat taaattatca atccccatct ggcgggcgat agcgtgcatt
    13141 ttctcctgct gtgccgtgtt cagcttcaat cctggattaa gccccaaccc gatgttctgc
    13201 gcgaccgtca ggtgactgaa caggttgttc tcctgaaaca gcatcgacac cggacggcgt
    13261 gacggcggcg tagtggtgtg atcaacgccg tcgatagtca gcgaaccgct ggctggcgtc
    13321 agaaaaccgg cgatcaaatt cagcagggta cttttacccg cgccgcttgg cccgaggatc
    13381 gccacctgct cgccgcgttc caccgttaag ctaaaacgca tcggcaaatg gtggtaaagc
    13441 caggtgatat cagtcagttt taacatttcg ccccggtagt ttttcaatca cagtaaacag
    13501 cagaaaacag agtagcagca gaattaacgc ggtgaccgcg ccgtcctggc tgcgatagga
    13561 gccaatttgc tggtagagat aaaacggcag ggtacggaaa tcatcgttac cgaacaacgc
    13621 caccacgcca aaatcaccaa tcgatagcac acaagcaaag gccagtgcct gcgccagtgg
    13681 gcgtttcagg gcgcgcagct ccaccacttt taagcgtgac cagccttcaa tgcccagcga
    13741 ctgacacaac atactgtaac gcgcggtgat atcgcgcatc gggttttcca gcaccttcag
    13801 cgcataaggg atcgccatta acgcattggt gaaaatcaca atgccgtcag cagattgcgg
    13861 caggccgata gtgttgttga gcagtaaaaa gaagcccgta gccagcacaa tccccggcat
    13921 ggcgaggatc aacatgccgc tcatctccag cgcctgaccc gccagcattt tttgccgcac
    13981 ccgcagttcg cgactgctcc atagcagcat catggtcagc actacgcata atacgcctgc
    14041 cgccagcgca atacgcaacg aggtccacag cgcctgccac agcaccggtt gtgccagcac
    14101 ttccggcaac tggcgattta ccccatcgac gatcaccgcc agtaacggtg gtagcaacag
    14161 cagcagcgcc agcacaatta acaccgtgtc gcaaatgcgg ctatgcagac gatcgtccgg
    14221 gtcgcgccag ccttgcagca gagtagtgcc gggcgcaatg gccttactca atcgctgact
    14281 caacagcact agcccgaggc aacacaccat ctggatcaac gccagcatcg ccgcgcgggc
    14341 aggatcgtag tcgtaactca gcgcctgata aatagccagc tcgatagtgg tcgcctgcgg
    14401 accgccgccc agcgacagca cggtggcgaa gctggcgaaa cagagcataa agataagcgc
    14461 agcaactggc gggatttgtc gccgtaacca cggccattcg acgaagcgga aaaaatgcca
    14521 gccgcgcatc ccaagctggg cggcaagctg acgttgttcg ccgggaatgt tttccagtgc
    14581 ctggagtaat aagcggctcg ccatcggcag attaaaaaac acatgggcca gcaaaatgcc
    14641 ttgcaggccg tagggcgaaa aggtccactc cagaccgagc gattggcaga gcgatgccag
    14701 ccagccctgg cgaccataga cgctaagaat gccgaaaaca gcaaccagca ccgggaggat
    14761 caaggtcatg gcacacagac gcaacagcgc cagccgtcct ggaaagcgcc tgcggtagag
    14821 ggcgcgggca aggaatatcg cgggcacgac agagagcagc gccgagagaa acgcctgcca
    14881 gaaggagaag cgcaccacat gccacagata gctgtcctgc cagactgcca cccagtcacc
    14941 ctgcggcgcg ttccaccaca gggcgagaaa cgccgccagt gcaaccgcca ccacaagcgt
    15001 agcggcgctt acacctggaa ttaaccagcc gggaattaat ggctgacggc gcgttgccat
    15061 tcgctaatcc atgcctgacg ttgtgccgcc acttcggctg gcgtgaactc cagcgtggtt
    15121 gccggtttgg tcaattgttc aaacccggca ggcagcgtga catttgccac cggatacatc
    15181 cagttgccgg ttgggatcgc attctggaaa gccggagaaa ccataaactg gaggaatttt
    15241 tgcgccagct ctggctgctt gctggcagcg gtgcgggcgg cgacttccac ttgcaaatag
    15301 tgaccttcgc tgaagttcgc ggtggcgtag ttatctttct tctcttcgag aatgtgataa
    15361 gccggagagg tggtgtaact caggaccaga tcgctttcac cttttaaaaa caggccgtag
    15421 gcttcgctcc agcctttggt gaccgtgacc gttttcttcg ccagtttctg ccaggcttgt
    15481 ggggcgtcat cgccatagac tttttgcatc cacaacagca gacccagtcc cggcgtgctg
    15541 gtgcgcggat cctgataaat cacccgccag ttttgatcgc tctcaaccag ttctttcagg
    15601 ctttgcggcg gatttttcag tttgttcttg tcatagacga aggcgaaata gccatagtca
    15661 aacggcacaa aggtgtcatt tttccagccg ccgggaacgt taacggcttc cgctgccaca
    15721 ccgcttttag caaacaaccc ggttttgctg gcggcgtcca gcaggttgtt atccagcccc
    15781 agcaccacat cggctttgct gtttttgcct tccatccgta gacggttgag cagcgaaacg
    15841 ccatcttcca gcgccaccag tttcagttcg caattacagt ctgcttcaaa ggcttttttg
    15901 accaccggac caggtcccca gtcagcggca aaggaatcgt aggtataaac agtcagaacg
    15961 gatttagcga aaacaggcgc tgtgcacagc aacagcaggg gaagacattt ttttaacact
    16021 ttgcacctca aaaaagagtg gcaaaggact tgagaaggag cctcaaatcc cttcgccggc
    16081 gttatccgga tcaggttcga cgggtatttt ctcagcgcac gcggctgcgt ggcaccccgt
    16141 tgagaacggc gttagtgtag tgattttgtt atcaaccagc aatccataga gaaggtgtat
    16201 taaaaaagcc aatattcgtt cagctatcaa ggacgtattt gcatagctga agggtgtcga
    16261 tccgaaagaa tcccttagct tcagtcagaa tcggtaacgt aacagtttat tgcgtgaatg
    16321 agcgattttt gacagtattc ttattcagga cgcttattct gagtttttaa ctcagagtgg
    16381 agctatcagc atgaaagtaa gaaacccaga acagattagt atccctgcaa gtaataccac
    16441 taaagatcca ggccttactc attctcagat tatcaggatg gttaacctgg tcaaaaagac
    16501 cgagaatatg aatatctttg aagagctatg ggagacgtta cgcaatctct tccggtcaga
    16561 taaacattcc cagactgcgg caaggcaaat tctgaaggat gcattttatt tccagaattg
    16621 tgatgactac tcaaaatact ttaccggggc tgtagatggg gatgcgcgtg ataagtttac
    16681 ccactggctg ataaatttta ataaactcaa agaacatgca atagatccgg aaaatatggc
    16741 cgcgaaggcc tcactctcgt caggagatac gttaagagtc agttttttta ttggagatga
    16801 ggcgatactt actctggatt tgcagctgaa agagagcacc agtaccgggt gcattgatct
    16861 gagtcatgct tactttaacg gggttgtcat ttgtggtatt gaccgtctcg aggttgacct
    16921 gagcaatgct gaaacgaaca atagccgttg gtatgactga cctgcgcgag gttaacctga
    16981 aaaagctgac ctttccggcc agaacacggc taggctgcgc cgttattgat taactcagaa
    17041 tatgaaagag gggctggaat gccccttctt taatcttatt tgcgactcta atcagacgat
    17101 gcataaaaca gagaaaatat aaaagtaatg tattttattc attccgtctg aatataaaaa
    17161 acgcgctcat gaccgccaaa aggcacaagc tactctggat ccggcggcgc aaaccacgct
    17221 gatttaaaat cgaaccagcc gagggtgttc atacgcagac cgcgcatact gcgttgcccc
    17281 tgaatgatca gccagtggtg gataagtggc accattgctt tactggcgac cagttgctgg
    17341 caccagttcg ccagattcat ttcgccattg cgccagcggg cggcgtcggc ttgccagtcg
    17401 atcggaatac agtgttggag cagcggcacc tcgcacaggt gcgcgaacag cgaaaaatcc
    17461 agcggcagcg taaagttggc gctgttaagc cagatatcgc tctcgatctc tccttcatgc
    17521 cactgatcgt agctgatctc tttgatttcc agcgtgacct ggtgacttgc cagaatctgc
    17581 tgcatgatcc cggcaatcac tcgatgctca atatgatcct gataaaaggt cagggtgagg
    17641 ctttccagcc cggcgggttt ttcgcaatgt gtcggacgag catggtgcca acgtgggagc
    17701 agtccataag ccgggaacca cagttgctgg tactgttcct cagcgaaata gaccagatta
    17761 gttggagaaa gcacatagct tacccagtcc ctgacttgct gattcgcccc gcgatgggtg
    17821 cggttgtcga acagtaaata gtagcaacct tcctccaggc ggctttcaat ctctttttcc
    17881 tcgccctgtg gaccttttag catcagccct ccggctggct cgtcggcaat ttccggtaga
    17941 acccagacgt taacttcgtc gattaatgcc cggtaaccga agaagtcatc gaatgcctga
    18001 attttcagtt gattggtgct gttgcggatc accccatacg gaccggtgcc gatgggatgg
    18061 ctggcaaagt tactgatggt ttcccattcg cgcggcagga tcatcgccgg aacttgtccc
    18121 agcagtaacg gcaaccagcg gtccggctgc gtgaggtgga tatccagcgt ccagggcgtc
    18181 ggcgatacaa tttcagcaat atgcgaatag agcggcagcg tattgatccg ttttaaagag
    18241 gcgatcacat cgtccatttc cagttcacga ccatgatgaa aatggactcc tggacgcaaa
    18301 aagaaacgcc agtgaagcgg tgatatttgc tgccagtggt gggctatgtc cgcttccagt
    18361 tccccatttt cctcatttat gcgcgttagc gaactgaaga tttgccgggc gatatgggtt
    18421 tcggaacgac gcaatgcgct gccaggtagc agattacgca acggacgata gtagagcacg
    18481 cgcaggatgt gccgcccctg gcggaagctg cggcccagat gagaaaccag catttgccgc
    18541 acagtcgctt tgtcgccaac caactgcact agttggtcga tacgatcctg ctccagcagg
    18601 tcttccgccc gctgttgctg aagtgccagc ccggtataga ggaaggtcag acgcgagcgt
    18661 ttaccgcgcc cgacttccgc ttcccacgtc agccagccgc gatcctgcat ggtgttgagc
    18721 agggtacgca tatgacgacg cgagcagctc aataacgctg ccagttcgtt gagcgtcgtg
    18781 tcctgcgatt taccctcgca gcattgccac aggcggatga actgttgttg cagacgagca
    18841 gatggcataa aaggggaact cctgcgcaaa agtcagcaat tttattttcc ctatattaag
    18901 tcaataattc ctaacgatga agcaaggagg tgccccatgc gtcagtttta tcagcactat
    18961 tttaccgcga cagcgaagtt gtgctggttg cgttggttaa gcgtcccaca acgattaacc
    19021 atgcttgaag aactgatgca gtgggagggg agtcattctg gctactgatt tgcggacatc
    19081 atgtgtgact gagtattggt gttaatcgcc acgccagcag tgattatctg ctggcttttt
    19141 tctttttggc gattgagtgc cggacggttt acaaactaac gcataaattc cggttgcttc
    19201 tcttcataag acgcaatagc gtcgtcgtgc tgcagggtca ggccaatgct gtccagacca
    19261 ttcatcatgc agtggcggcg gaaggcatca atggtaaatc gataagtttt ctctcccgct
    19321 ttcacctctt gcgcttccag gtccacgtcg aaataaatcc ccggattggc ctgcaccagc
    19381 gcaaacagtt cgtccacttc tgcgtcgctt aatttcaccg gcagcagctg gttgttaaag
    19441 ctgttgccgt agaagatgtc agcaaaactc ggggcaatca ccactttaaa accgtaatcg
    19501 gtcaatgccc acggcgcgtg ctcacgcgag gaaccgcagc cgaagttttc ccgcgccagc
    19561 aaaatggaag cgccctgata ctgcgggaag ttcagcacga agtccgggtt tggctgttgg
    19621 cctttttcat ccagaaaacg ccagtcgtta aacagatgcg cgccaaaacc tgtacgggtc
    19681 actttctgca aaaactgttt cgggatgatt gcatcggtat cgacattggc ggcatccagc
    19741 ggaaccacca ggcctgtgtg tttgataaat ttctctgcca tggtgtgctc cttatttaat
    19801 gttgcgaatg tcggcgaaat gtccggtcac agcagcagcg gcagccattg ccgggctgac
    19861 cagatgggtg cgccctccac gcccctggcg gccttcaaag ttacggttgc tggtggaggc
    19921 acaacgctca cccggattca ggcgatcgtt gttcatcgcc agacacattg agcaacctgg
    19981 caagcgccat tcaaaaccgg cttcaataaa gattttgtcc agaccttccg cttccgcctg
    20041 ggcttttacc gggccagagc cgggaaccac cagtgcctgc acgcctggcg cgactttccg
    20101 ccctttggcg atttcagccg ccgcgcgtaa atcttcaata cgcgagttag tgcaggaacc
    20161 gataaacact ttgtcgatag ccacttctgt cagcggaata cccggtttca gccccatata
    20221 ggccagcgct ttttctgccg acgcgcgttc aaccggatcg gcaaacgaag ccggatcggg
    20281 aatattgtcg ttcacggaaa tcacctgacc tggattggtg ccccaggtga cctgcggtga
    20341 aatttcttct gcctgcaaag tcacaacggt atcgaaagtt gcgccttcgt cggtttgcag
    20401 ggttttccag taggcaacgg cgttgtcgaa atctttgcct ttcggcgcat gcagacggcc
    20461 tttgacatag ttaaaggtag tttcgtccgg tgcaaccaga ccggctttcg cgcccatttc
    20521 gattgccata ttgcacaggg tcatacgacc ttccatgctt aaatcacgga ttgcttcgcc
    20581 gcaaaactcc accacatgcc cggtgccgcc tgcgctgcca gttttaccga taattgccag
    20641 cacgatatct tttgcggtaa tgcccggcgc ggctttgccc tggacttcaa ttttcatggt
    20701 cttcgcacga ccctgtttca gggtttgcgt tgccagtacg tgttcaactt cggaagtgcc
    20761 gataccaaag gccagtgcgc caaacgcgcc gtgggtggcg gtatgcgagt cgccgcagac
    20821 aatagtcatc cccggcaatg tgacgccctg ttccggcccc attacatgga cgatcccctg
    20881 atacgggtga ttcaggtcat acagctcgac gccaaactct ttgcagtttt tgatcagctc
    20941 ctgcatctgg atgcgcgcca tttcaccgca ggcattaatg tctttcgtct gggtagagac
    21001 gttgtgatcc atggtggcga aggttttgcc cggctgacgt accgggcgac cgtgggcgcg
    21061 cagaccatcg aacgcctgcg gtgaggtcac ttcatgcacc aggtggcggt cgatatataa
    21121 cagcggggtt tcgttttcgg cttcgtacac tacgtgagcg tcgaacaatt tttcgtataa
    21181 cgtcttagcc atgattacac cccttctacc acatagcggg caatgatatc gcccatttca
    21241 ttggtactaa cggcggcagc gccacgggct aaatctccgg tgcgaatgcc ttcttctaat
    21301 gcgcggttaa tggcgcgttc aatggcggaa gccgcatcat cggcatccag gctgtaacgc
    21361 agcagcagtg ccagcgacag gatttgtgca atcgggttgg cgatgttttt gcctgcgata
    21421 tctggtgcgg agccgcctgc cggttcatac agaccaaaac cttgttcgtt caggctggcg
    21481 gaaggcaaca tccccattga gccagtgatc attgcgcact cgtcagacag aatgtcgcca
    21541 aacaggttgg agcacagcag gacgtcaaac tgtgatggat ctttaatcag ctgcatggtg
    21601 gcgttgtcga tgtacatatg cgccagttcg acatccgggt attccgtggc gatctcgtta
    21661 acgatctccc gccataaaat agaggattgc agcacgttgg ctttgtcgat agacgtcact
    21721 ttatggcgac gtttacgagc agattcaaac gcgatgcggg cgatacgttc aatctcaaaa
    21781 cggtgataca cctcggtatc aaacgctttt tcatattgtc cgctaccttc gcggcctttt
    21841 ggctgaccga aatagatgcc gccggtcagt tcgcgcacac acaggatgtc gaagccgtta
    21901 gcggcaatgt cagcacgcag cggacagaat gcttccagcc cctgatacag ttttgccgga
    21961 cgcagattgc tgaataattt gaagtgttta cgcaaaggca acagtgcgcc gcgttctggt
    22021 tgctggtctg gcggcaaatg ttcccacttc ggaccaccta ccgagccaaa cagcacggca
    22081 tcggcttgct cacaaccttc aaccgtcgca ggcggcagcg gctgcccgtg gttatcaatg
    22141 gctgcgccgc ctacatcgta atggctggtg gtgatacgca tcgcaaagcg gttgcgcacg
    22201 gcatccagca ctttcagcgc ctgggtcatc acttccggac caataccgtc ccccggcaat
    22261 acggcaatat ggtaattctt cgacatcaca cggtttcctt gttgttttcg ttgtgttgag
    22321 ctttgcgttg caactctttt tcgacttctg tggcacgcca gatattgtta agtacgtgca
    22381 ccatggcttt ggcggaggac tcgacaatat cggtggccag gccgacgccg tggaagcggc
    22441 gaccattgta gttggcgaca atatccacct gacccagcgc atctttaccg tggcctttgg
    22501 cggtcaggct gtacttcacc agttcgacgt tatagtcagt gatgcggttt atcgcctgat
    22561 agacggcatc gaccggaccg ttaccgttgg cggcttctgc tttgacttct tcgccgcagg
    22621 ccagtttgac ggcggcagtg gcaatatcgt tagagccaga ctgcacgctg aagtaatcca
    22681 gacggaaatg ctccggctct tcttgctgct taccgatgaa ggccagtgcc tccagatcgt
    22741 aatcaaacac ctgacctttt ttgtccgcca gcttcaggaa ggcatcgtac aaattgtcta
    22801 aattatattc actttcttta taccccatct catccatgcg gtgttttact gccgcacgtc
    22861 ccgaacgaga ggtcagattc agctggattt ggttcaaacc aatagattct ggtgtcatga
    22921 tttcgtagtt ttcgcggttt ttcagcacac catcctgatg aataccggag gagtgtgcga
    22981 atgcgccgct gccaacaatg gctttgtttg ccgggatcgg catattacaa atctggctaa
    23041 ctaattggct ggtgcgccat atttcctggt gattaatggc ggtatgaacg ttgagaatat
    23101 ccttacgaac tttgatcgcc atgatcactt cttccaggga acagttaccg gcacgctcgc
    23161 cgatcccgtt cattgcacct tccacctggc gtgcaccggc atgtaccgcc gccagtgagt
    23221 ttccgaccgc caggcccaaa tcgtcgtggg tatgtacgga aataatggct ttgtcgatgt
    23281 tgggtacgcg ttcatacagc ccgctgatga ttccggcgaa ctcaaatggc atggtgtagc
    23341 ccacggtgtc cgggatgttg atggtggtgg caccggcatt aatcgccgct tcgaccactc
    23401 gcgccagatc ggcaattggg gtgcgtccgg catcttcgca agaaaattca acatcatcgg
    23461 tgtaattacg ggcgcgtttc accatataga tagcgcgttc gatcacctcg tccagcgtgc
    23521 tacgcagctt ggtggcaatg tgcattggcg aagtggcaat aaaggtatga atccggaagg
    23581 cttcggcgac tttcagagat tcggccgcca cgtcgatatc cttttccacg cagcgagcta
    23641 acgcgcatac gcggctgttt ttaacctggc gagcgatggt ttgcactgat tcaaaatcac
    23701 ccggcgaaga gacggggaac ccgacttcca tcacgtcaac acccatacgc tcaagggcca
    23761 gcgcaatttg cagtttttct ttcacactca agcttgcctg taacgcctgt tcaccgtcgc
    23821 gcaatgtggt atcgaaaata atgacttgct ggctcatggt ttgggtcctt gtctcttttt
    23881 gagcgcctcg cttcgggcat aaaaaaaccc gcgcaatggc gcgggttttt tgtttgactg
    23941 cgtgctggct taatgctgga tgccgctcac tcgtctaccg cgcaaagaag atgcgtttag
    24001 tagtagtaga ccgataaagc gaacgatgtg agtcattaaa tcagctccag atgaatgcga
    24061 tatgctttta gagttactgg atacaaaaac ggatgtcaac cctgacgtaa taaaaacgtc
    24121 ccgccagcat gagttctgca tccgtcaaat tagctaattg tgctgcggtg gttaaaacaa
    24181 gcgatattaa tttctgctta accaccgccg cttttcatcg gttgacatat ttcagaataa
    24241 atttttgcat ctaatcgacg agggaaaggg ggataaaatg cacgcgttac gaaacctatc
    24301 ctgatgattt gtattgaatt atatgttttg cgaatttttt gatattgatt tggtgaatat
    24361 tattgatcaa ttaatgttaa ggattaatgc attaaacata taaattaatt attaaaaaag
    24421 cacatttaat ccattttgtg gatgattgag tattcgcggt agttatga
//
LOCUS       DAOTRK010000067        24353 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.067, whole genome shotgun sequence.
ACCESSION   DAOTRK010000067 DAOTRK010000000
VERSION     DAOTRK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.067"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(100..2367)
                     /gene="adiA"
                     /locus_tag="QIE41_002553"
     CDS             complement(100..2367)
                     /gene="adiA"
                     /locus_tag="QIE41_002553"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDS3531430.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGITRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSSQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHIMCVKA"
     gene            complement(2566..3474)
                     /gene="melR"
                     /locus_tag="QIE41_002554"
     CDS             complement(2566..3474)
                     /gene="melR"
                     /locus_tag="QIE41_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDS3531431.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3757..5112
                     /gene="melA"
                     /locus_tag="QIE41_002555"
     CDS             3757..5112
                     /gene="melA"
                     /locus_tag="QIE41_002555"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDS3531432.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5215..6636
                     /gene="melB"
                     /locus_tag="QIE41_002556"
     CDS             5215..6636
                     /gene="melB"
                     /locus_tag="QIE41_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDS3531433.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6775..7404)
                     /locus_tag="QIE41_002557"
     CDS             complement(6775..7404)
                     /locus_tag="QIE41_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDS3531434.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGHKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7527..9173)
                     /gene="fumB"
                     /locus_tag="QIE41_002558"
     CDS             complement(7527..9173)
                     /gene="fumB"
                     /locus_tag="QIE41_002558"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDS3531435.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILQDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9251..10591)
                     /gene="dcuB"
                     /locus_tag="QIE41_002559"
     CDS             complement(9251..10591)
                     /gene="dcuB"
                     /locus_tag="QIE41_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDS3531436.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11162..11881)
                     /gene="dcuR"
                     /locus_tag="QIE41_002560"
     CDS             complement(11162..11881)
                     /gene="dcuR"
                     /locus_tag="QIE41_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDS3531437.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11878..13509)
                     /gene="dcuS"
                     /locus_tag="QIE41_002561"
     CDS             complement(11878..13509)
                     /gene="dcuS"
                     /locus_tag="QIE41_002561"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDS3531438.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13689..13919
                     /locus_tag="QIE41_002562"
     CDS             13689..13919
                     /locus_tag="QIE41_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDS3531439.1"
                     /translation="MDQDLLDGGYRCYTGEKIDVWFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSGALKYRHK"
     gene            13931..14203
                     /locus_tag="QIE41_002563"
     CDS             13931..14203
                     /locus_tag="QIE41_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS3531440.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14432..14728
                     /gene="ghoS"
                     /locus_tag="QIE41_002564"
     CDS             14432..14728
                     /gene="ghoS"
                     /locus_tag="QIE41_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDS3531441.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14756..14929
                     /gene="ghoT"
                     /locus_tag="QIE41_002565"
     CDS             14756..14929
                     /gene="ghoT"
                     /locus_tag="QIE41_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDS3531442.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15048..16565)
                     /gene="lysS"
                     /locus_tag="QIE41_002566"
     CDS             complement(15048..16565)
                     /gene="lysS"
                     /locus_tag="QIE41_002566"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDS3531443.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERIFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16802..18259)
                     /gene="dtpC"
                     /locus_tag="QIE41_002567"
     CDS             complement(16802..18259)
                     /gene="dtpC"
                     /locus_tag="QIE41_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDS3531444.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFAARFLFSTPTNMIQESND"
     gene            complement(18318..20465)
                     /gene="cadA"
                     /locus_tag="QIE41_002568"
     CDS             complement(18318..20465)
                     /gene="cadA"
                     /locus_tag="QIE41_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDS3531445.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSNWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20545..21879)
                     /gene="cadB"
                     /locus_tag="QIE41_002569"
     CDS             complement(20545..21879)
                     /gene="cadB"
                     /locus_tag="QIE41_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDS3531446.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22245..23783)
                     /gene="cadC"
                     /locus_tag="QIE41_002570"
     CDS             complement(22245..23783)
                     /gene="cadC"
                     /locus_tag="QIE41_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDS3531447.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         6266 a         5744 c         5993 g         6350 t
ORIGIN      
        1 tgtttgcagc aatttttatg aaaacgttgc catacttaaa aggcctcaag ccaggcggcg
       61 taatgttatt taacaattac gccttcagcg gaatagtggt tacgctttca cacacataat
      121 gtggtaaata ccgtcaataa tttctgtccc ttctgtttcg tgttcaaacc cagggaaatg
      181 atggtcccag gattgcagcg agcgtaaata acttacttgc gaactgtttt tatcgccgaa
      241 gttttcacca gacagcagca tcgggattcc tggcggatac gggataactg agtttgccgc
      301 gatgcgtcct ggcagatttt caatggatac cagttcgaca ttgttgtcga caatcgcgtt
      361 gtacgcttca cgtggggtga tttccgccac cggcaggccg gaataggctt cgttcaaccg
      421 tgcgccaggg ttgttttctt tcagccaggc aaacatggtg tcacccagat cgtgaatccc
      481 catgttcgcg taagtgtcag gatattgttc aacaagttcc ggcatcacct gcgccagcgg
      541 tgtgttggcg tcatagtggc gtttgaagga gcaaagggtg ttaaccagag tcccccattt
      601 cccacgggtt atgcccatag agaacaggaa catgatttgg aagtcagtgg tgcgggtagg
      661 cacaatgccg tggcgaccaa gccaggcagt gaccagcgcc gccggaacac cggtttcttc
      721 cagttcacca tcttcaccca ttcccggagc aaggatgctg actttaattg ggtcgagcat
      781 actccagtta tccggaatat ctttgaagcc gtgccagctt tcgcccggat gcatgaccca
      841 gcagtcctga acggtggtca gcagtttggt tggtgcgtca gcaaagtcat aggttttgcc
      901 ggtttgtggg tcggtgacga cttctttgtt ccacggtttg aagaaccagc taccgtcagc
      961 ggtgaactct ttatacagcc gcgccatcgc ctgacggaaa tcaaccgctt cgtcgatcac
     1021 ttcctgagtc agtgacaggc cgctgttgcc gtccatcatc gacaccgcca cgtcgttgga
     1081 tgcgcagatg gcatacagcg gggaggtggt ggcatgcatc atgtaggcct ggttgaagcg
     1141 ggagaagtta atcgccccac gaccttcacg tacatgaata taagaggcct gtgacagcgc
     1201 attcagcagt ttgtgggtgg agtgggtggc gaaaacggta gggccgttgt gatcgccagg
     1261 ttcgccgcgc atggcatagt gatcggcata gatcgggttg aaacgtgcat agccgtacca
     1321 ggcttcgtca aagtgcagac gatcggaggt tttttccagc agatcctgcg cttctttagc
     1381 gttataacac acgccgtcat aggtgcagtt ggtcaccacg cagtaagacg gtttttgccc
     1441 ggctttgtct ttagtcagcg ggctttcact gattttcttc tgcaaggttt caggttgcat
     1501 ttcctgcgga tagattggcc cgataatgcc gtagcggttg cggcttggca ccatgtagac
     1561 cggtttcgcg cctgtcagca tcaagccttg ttcgatggat ttatggcagt tacggtcaac
     1621 gaccacgaca tcgttatcgg tcatgcaagc ctgcatgatg gtgcggttag agccggaagt
     1681 accgacgact accgaccagg agcgatcggc accaaatacg cgtgcggcat atttttcgct
     1741 ttcgccaaat gcgccagtat ggtcaagcaa agaaccgagg gaagtgcgtt cgatgcccat
     1801 gtcggtgcgg aacaggtttt caccatagta gtcatggtag aaacgtccgg cgggtgtttt
     1861 ggtaaaacca acgccaccct ggtggcctgg cgctgcccag gaatattcat ggatgtcgct
     1921 atatttcatc agcgcgctga acagtggcgg caacagctgc tggcggtagc gggtcatcgc
     1981 ggcaacggcg cgtccggcga taaagtcggc ggtatcttcc agaatccagg cgaattcatc
     2041 gacaagctcc agcagatccc ggtccattgc ggcgagggct ttttcccgat cgcccaacag
     2101 gaagaccggc acgttttgtt ggcgctcatg aagcttaccg atcaattgtc tgacgttttg
     2161 atgttcgtcc ggatgttcca tttgatagct gaacatcagg cagtcaatgg cttcgtttga
     2221 agagagaatg gcaaaaccat catcaaagga ggtggattta atcacggtaa cattttgctg
     2281 gcttaaagca tctgccagac gctcaacggc gttaccgacc caggtgtctt gatggagaaa
     2341 ctcgctttca acaattaata ctttcatcat tgcttacccg gttatgaagg aatcttcgtt
     2401 gcgggcaagt atcttccggc cttttcgggc tgtaaagcgc gtgaaaaaca aacatttttg
     2461 aaatatttat ttttataaac aatatgttat gtgattattt taaattattt tcactgctta
     2521 ttatcgacgg ctaaactatt tttttggctg atactgatat cgtctttagc cgggaaacgt
     2581 ctggcggcgc tgttggctaa gtttgcggta ttgttgcggt gacatgccga catatttgcc
     2641 gaacgtgctg taaaaacgac tacttgaacg aaagcctgcc gtcagggcaa tatcgagaat
     2701 acttttatcg gtatcgctca gtaacgcgcg aacgtggttg atgcgcatcg cggttatgta
     2761 ctgtttcatc gtcaattgca tgacccgctg gaatatcccc attgcatagt tggcgttaag
     2821 tttgacgtgc tcagccacat cgttgatggt cagcgcctga tcatagtttt cggcaataaa
     2881 gcccagcatc tggctaacat aaaattgcgc atggcgcgag acgctgtttt tgtgtgtgcg
     2941 cgaggtttta ttgaccagaa tcggttccca gccagagagg ctaaatcgct tgagcatcag
     3001 gccaatttca tcaatggcga gctggcgaat ttgctcgttc ggactgttca attcctgctg
     3061 ccagcggcgc acttcaaacg ggctaagttg ctgtgtggcc agtgatttga tcaccatgcc
     3121 gtgagtgacg tggttaatca ggtctttatc cagcggccag gagagaaaca gatgcatcgg
     3181 cagattaaaa atcgccatgc tctgacaggt tccggtatct gttagttggt gcggtgtaca
     3241 ggcccagaac agcgtgatat gaccctgatt gatattcact ttttcattgt tgatcaggta
     3301 ttccacatcg ccatcgaaag gcacattcac ttcgacctga ccatgccagt ggctggtggg
     3361 catgatatgc ggtgcgcgaa actcaatctc catccgctgg tattccgaat acagcgacag
     3421 cgggctgcgg gtctgttttt cgtcgctgct gcacataaac gtatctgtat tcatggatgg
     3481 ctctctttcc tggaatatca gaattatggc aggagtgagg gaggatgact gcgagtggga
     3541 gcacggtttt caccctcttc ccacagcggc gaggggagcg tccgagcatc atgaggccga
     3601 aaactctgct tttcaggtaa tttattccca taaactcaga tttattgatg cttcacgtag
     3661 gatctgagtt tatgggaatg ctcaacctgg aagccggagg ttttctgcag attcgcctgc
     3721 catgatgaag ttattcaagc aagccaggag atctgcatga tgtctgcacc caaaattaca
     3781 tttatcggcg ctggttcgac gattttcgtt aaaaatattc ttggtgatgt gttccatcgc
     3841 gaggcgctga aaacggcgca tattgccctg atggacattg atcccactcg cctggaagag
     3901 tcgcatattg tggtgcgcaa gctgatggat tcagcagggg ccagcggcaa aatcacctgc
     3961 cacacccaac agaaagaagc cttacaggat gccgattttg tcgtggtggc atttcagatt
     4021 ggcggttatg aaccttgcac ggtgactgat ttcgaggtct gtaagcggca tggtctggaa
     4081 caaaccattg ccgatacgtt ggggccgggc ggtattatgc gcgcgctacg taccattccg
     4141 catctgtggc aaatttgcga ggacatgacg gaagtctgcc ccgatgccac catgctcaac
     4201 tacgttaacc caatggcgat gaatacctgg gcgatgtatg cccgctatcc gcatatcaaa
     4261 caggtagggc tgtgccattc ggtgcaggga acggcggaag agctggcgcg tgacctcaat
     4321 atcgacccgg ctacgctgcg ttaccgttgc gcaggtatca accatatggc gttttacctg
     4381 gaactggagc gcaaaaccgc cgacggcagt tacgtgaatc tctacccgga actgctggcg
     4441 gcttatgaag cagggcaggc accgaagccg aatattcatg gcaatactcg ctgccagaat
     4501 attgtgcgct atgaaatgtt caaaaagctg ggctacttcg tcacggaatc gtcagaacat
     4561 tttgctgagt acacaccgtg gtttattaag ccaggtcgtg aggatttgat tgagcgttat
     4621 aaagtaccgc tggatgagta cccgaaacgc tgcgtcgagc agctggcgaa ctggcataaa
     4681 gagctggagg agtataaaaa cgcctcccgg attgatatta aaccgtcacg ggaatatgcc
     4741 agcacaatca tgaacgctat ctggactggc gagccgagtg tgatttacgg caacgtccgt
     4801 aacgatggtt tgattgataa cctgccacaa ggatgttgcg tggaagtagc ctgtctggtt
     4861 gatgctaatg gcattcagcc gaccaaagtc ggtacgctac cttcgcatct ggccgctctg
     4921 atgcaaacca acatcaacgt acagacgctg ctgaccgaag ccattcttac ggaaaatcgc
     4981 gaccgtgttt accacgccgc gatgatggac ccgcatactg ccgccgtgct gggcattgac
     5041 gaaatatatg ctcttgttga cgacttgatt gctgcccacg gcgactggct gcctggctgg
     5101 ttgcaccgtt aaaacgcgac taaacgctac tgcgccgggg gatttattcc ggcgcacact
     5161 tctgacgata ccaataacag aaggcgggcg ttggtaacaa cgacccgata ccctatgagc
     5221 atttcaatga ctacaaaact cagttatgga tttggagcgt tcgggaagga ttttgcgatc
     5281 ggcattgtgt atatgtacct catgtattac tacaccgatg tcgtcgggct gtctgtgggt
     5341 ttggtcggta ctttgtttct ggtggcgagg atctgggatg ctattaacga tccgattatg
     5401 ggatggattg taaatgctac gcgatcgcga tggggtaagt tcaaaccctg gatcctgatc
     5461 ggtacgttgg caaactctgt aatcttattt ctcctcttta gtgcgcatct gtttgaaggt
     5521 actactcaga ttgtctttgt ttgcgtgacc tacatcctct ggggcatgac ttacaccatt
     5581 atggatattc ccttctggtc gctggttcca accataacgc tcgataaacg tgagcgcgaa
     5641 caactggttc cttatccgcg tttttttgcc agtctggcgg gctttgttac ggcaggtgtg
     5701 acgctaccat ttgttaatta tgtcggcggt ggcgatcggg gatttggctt tcagatgttc
     5761 actctggtac tgatcgcctt ttttattgtt tcaaccatca tcactctgcg caatgtgcat
     5821 gaagtctttt cgtcagacaa tcaaccgtct gctgaaggaa gccatctgac acttaaagcc
     5881 atcgttgcgc ttatttataa aaacgatcag ctttcatgcc tcttgggtat ggctcttgct
     5941 tataatgtag ccagcaatat tattaccggc tttgctatct attatttctc atatgttatc
     6001 ggtgatgcgg atttgttccc ctattatctg tcgtatgcgg gagctgctaa tctggtgacg
     6061 ttagtattct tcccacgctt agttaaatca ttatcccgac gcattttatg ggccggagca
     6121 tctattcttc cggtgttaag ctgtggtgtt ctcctgttaa tggcattaat gagctatcac
     6181 aacgtcgtcc tcattgtgat tgcgggtatc ttgctgaatg tgggaacggc gcttttctgg
     6241 gtattacagg tcatcatggt ggcagatacc gttgattacg gtgaatataa actgcacgta
     6301 cgctgtgaaa gcatcgctta ctccgtgcag actatggtgg tgaagggcgg ttcagccttt
     6361 gcggcttttt tcattgcggt tgtgttaggg atgattggct atgtaccgaa tgttgaacag
     6421 tctacgcaag ccctattagg tatgcagttt attatgattg ctctaccgac tctgtttttc
     6481 atggtaacgc tgattctcta cttccgtttc tatcgcctca atggtgacac gctgcgcagg
     6541 atccagatcc atctgctgga taaatatcgc aaagtaccgc ccgagcctgt tcatgctgat
     6601 attccggtcg gtgcagtgag tgatgtgaaa gcctgacgtg gacaatagct aacgaaaatg
     6661 gcctgatgcg atatgtttat caggccatgt cttaatgtaa tgctttggat ttgcaaattt
     6721 tgtagaccgg ataaggaatt cacgccgcat ccggcgtcaa caaagcgcac gttgttatcc
     6781 ggttatcaag ccaaagcgcc gtagctggcg gcaatgaaag cgagcgagaa atatcaccgt
     6841 cgttaatgcg ccaagcagcg cgcagaacat atcggattgc gtgtcccact ggtcgccctg
     6901 ggtgccgaga aaatcatccg ctccctggcc cattgccagc gccgcccacc actcgattaa
     6961 ttcatacatc gcgcttatcg ccagcgccac gcagcagacc agaaacgcca ccatcttatg
     7021 tccgcgaacg tacataccgc gcacaaggat ctctcgtgcc accagtgcag gcaccagccc
     7081 ctggaaaaaa tgccccagtt tgtcatacgg attacggctc aaccccagcc attcctgcac
     7141 ctcaaaacca acggggactt tcgcgtaggt gtattgtccg ccgaccatca ggatgatggc
     7201 gtgaaagaaa atgagcgtat agagcagcgg cgttaacgga taacgtctgg cggtggcaag
     7261 cagtagcggc acgacaataa tcaccggtgt cacttccatc agccaggtga gtttgtcatg
     7321 ggccgaaatg ccggtataaa tcaggattaa cgttagcgcc agtgcgccgg tgttaagaat
     7381 taacggcttg agtgtgcggg tcatggtgag ttcgcaagtc agggaaaata ctgactattc
     7441 accagcaagc gtgaaattac aatcgcaaaa gaggtggccc agggggatca cctggcagca
     7501 tgctgccagg cgctgggccg aagaggttac ttagtgcagt tcgcgcactg tttgttgacg
     7561 atttgctgga agaagtcgtt acctttgtca tcgaccagga taaacgccgg gaaatcttct
     7621 acttcgattt tccagatagc ttccataccc agctccggat aagcgacgca ttccagatgc
     7681 ttgatgctct gctgcgccag taccgccgcc ggaccgccga tgctaccgag gtagaagccg
     7741 ccgtgtttat gacacgcgtc ggtaacctgc tggctgcggt tgcctttcgc cagcatgatc
     7801 atgctgccgc cgtgggattg cagcagatcc acgtaggagt ccatacgacc tgcggtggtt
     7861 gggccaagtg aacctgatgg ataaccggca ggggttttcg ccggacccgc gtagtagatc
     7921 gggtgatctt tgatgtactg cggcagttct ttaccggcgt caatcagctc tttcagcttg
     7981 gcgtgtgcaa tatctcgacc cacaataatg gtgccggtga gcgacaagcg ggtggatacc
     8041 gggtattgcg aaagctgggc gaggatctct ttcatcgggc ggttaaggtc aactttcacc
     8101 gcttcgcctt caccggcctg gcgcagttct tccggaatgt actggcctgg gttgtgttcc
     8161 agtttttcga tccagatacc ttcgcggttg attttcgctt taatgttacg gtcagcggag
     8221 caggagacgc ccatgcctac cgggcaggat gcaccgtgac gtggcagacg gataacgcga
     8281 atgtcgtgcg cgaagtattt accaccaaac tgcgcgccaa gaccgagttt ctgggcctct
     8341 tccagcagtt cctgttccag ctggacatcg cggaacgcct gaccatgttc gttcccttcc
     8401 gtcggcagtt catcgtaata gtgagcgctt gctaacttga cggttttcag gttggtttcc
     8461 gcagacgtac cgccaatcac aaacgcgata tggtacggcg ggcaggctgc agtaccgagg
     8521 gtacgcattt tctcaacgag gaagtttttc agtttgccgg gagtcagcag ggctttggtt
     8581 tcctggtaga gataggtttt gttggcagag ccgccgcctt tagcgacaca gaggaatttg
     8641 tactcatcgc catctaccgc gtacaggtcg atttgcgcag gcaggttagt gccggtgttg
     8701 acctctttgt acatgtccag cgccgcattc tgtgaatagc gcaggttgtc ttcgatatag
     8761 gtgttataga cgcctttcga cagcgcttct tcatcaccgc caccggtcca cacgcgctgg
     8821 ccttttttac cgacgatgat cgcagtgccg gtgtcctggc aggtcggcag cacgcctttg
     8881 gcggcgattt cggagtttct taagaattgc agcgccacgt acttgtcgtt ttcgctggct
     8941 tctggatctt gaagaatagc cgcgacctgt ttctggtgtg ccgggcggag catgaaagaa
     9001 gcgtcgtgaa aggcttgctg cgccagcaga gtcagggctt ctggttccac tttcaggatt
     9061 gtttcgccgt cgaagtcggc aacgctaacg taatcggaag tgagtagata gtattcggta
     9121 ttgtctttcc ccatcgggaa aggtgcctgg tagataaagg gtttgtttga catagcttcc
     9181 agcctgtaac tctgtatttg ttattcgaaa atggcgtgcc gcgtagtcac gggcggcacg
     9241 caaagtgcat ttataagaac ccgtacatcg cggcgaagat ccagccgaag acgcacgata
     9301 cgctcacacc aatcaacccc ggcagaataa agctgtggtt gatgacgaag cgaccgatgt
     9361 gggtggtgcc ggaacggtca aactgaatcg ctgccagatc gctcggataa gtcggcagga
     9421 tgtaataacc gtagcaagcc ggtgctgaag ccacgatgta tgccggatca acgccgatcg
     9481 ccagcgcgac cggaacaatc gccgccagcg ccgcagcctg agagtttaca aacttggaaa
     9541 ccagcagcag aacaatggca taggcccacg gatactcttt caccatttca cccagtacgc
     9601 cctgaatttc agacatatgc gcaccgaaca tggtttcagc catccatgcg ataccgtaca
     9661 ccgccacgat ggcgatcata ccggaacgga agacttcgtt ttttgagata gacgcgggat
     9721 tggttttggt caggataata atcagcgccc cggtaagcag cataaacatc tgaataacca
     9781 gtaccatcga cagcggtttg ccaccgaagg atggacgcag gtccgaatca gcaccaagaa
     9841 gtgcgactac agcgattgcc ccgaggaaga tccacattgc aagccagttg cttttcggca
     9901 gttttttatc gagcagcgtc gcggtatcac cgtaaacata ctcacggttt tccggtacgg
     9961 agatgaattt ctggaactct tcgtctttat ccagatcttt accgcggaac cagctgaaga
    10021 taccgatcgc caggataccg attaacgtcg atggaatggt gattgccagc aggtcgagga
    10081 actcaagatg gcgaccatca aaggtgacat tacccagcat cgcaaccaga gacacgactg
    10141 caaccgacac cggactggcg ataatcccca tctgtgcacc gatagaactt gccgccatcg
    10201 ggcgttccgg acggatgttg ttcttaatgg cgacgtcgta gatgatcggc agaatggtgt
    10261 aaaccacatg acccgtaccg caaagaatgg tcagcgtaca ggtcacaaac ggcgcgacaa
    10321 ttgagacata tttcgggttg cggcgcagca gcttctcggc aatttgcagc atgacatcaa
    10381 gaccgcccga agcttgcaag gtcgccgatg ccgccaccac cgcaatgata accagcatga
    10441 catcaaccgg tggtttacct ggctgaaggt ggaagacgaa gaccagaatg accagaccga
    10501 taccgcctaa taaacccagc gcgataccac cctttctggc accataaaac agacatatca
    10561 gtattatgat aagttggata gtaaataaca tgtgtgaacc ctcgcgataa tcctatttaa
    10621 atttttgctg aatagatcac agtcacgttc tgttttgtat gaactgtttt cagagcctga
    10681 ctaaatatcc gtttggtcgt taccggcttt agcaaatacc tcacagtgaa tattggctga
    10741 ttaaagcagc ggcaaattta tgcactgtga taagcggctt tttcagagag aaaaactctc
    10801 tggcgatggg tttatagttt gtactgcgca gatgttaatg ttttgtttgc tattatctta
    10861 ttgatattta tcggtctatt ttttgttctc agggcagata tcattaggta tatagattaa
    10921 tctgatctac ccatttgtgg gtaaaaatac acataatgcg ggtgacataa tagttaatta
    10981 acttttgtta gcgttttgaa attaaaaaca ccgttcatct gaagagatat taatttttaa
    11041 cgatgatgga gggataatta tatttgatct ggcacaagtt ttactgatga agaatgtaac
    11101 ttgtgccagg aatattttgc attacagtaa taattattac ttacagataa ccagcgatcc
    11161 gttattggca atattgtttc agcagtgagt agtgttctgc ctgaatacgg taacggtaaa
    11221 ccggacgccc cgtgacgcca taatggatac tggtgaacaa gatgtggcag ttgaccagcc
    11281 agatgaggta tttacggcag gaaacacgcg aaatgttaac ctcgttagcc agctcgtcgg
    11341 ttgaaaactc atagtcctga tgcgcgtcaa tccactggca cagcgtgcgt aacgtctgcg
    11401 gcgttaagcc ttttggcaag cgacgaggat cctgctcgtt ggagctgctg ccgtggatta
    11461 gctgatcaag ctcggcctgg tcataatact gatgtttttc cagcgccatt ttcttttgcc
    11521 gccagccggt gagcgcctct tcaaagcggg aagcctggaa gggtttgatc aggtaatcca
    11581 cgacaccgta atgcagcgaa tctttaatgg ttgccgcatc ggctgcggag gagatgacaa
    11641 tcacatcact tttgcaacgc gcgttatgca ggacaggcag taaatcgagc ccgttctctt
    11701 tttgcatata gatatcgagc aatatcaggt cgataggcgt atcgctattg aagataatct
    11761 ctttggcttt ctccagcgtc gaggctgttc cacagcattg aaagcctggg atttgtgcta
    11821 cgtatcggcg attcagctcc gcgaccattg cgtcgtcatc gataattaat acattgatca
    11881 tctgttcgac ctctccccgt cccagggtat ctggacaaaa aattgtgtga aaatcccggg
    11941 ttccgattcc acggcgatgc tgccgccgag attttctacc tgttgtttga caagtgctaa
    12001 accgacgcct cgctcgcttc cttttgtcga gacacctttg tcaaaaatgt gatcgatttt
    12061 atcgggtgcg atccccggtc catcatcatt aacttcacag tgcagccagc cgtgacggta
    12121 gtgcaatgtt acgctaattt cgcctccggg ttccggccct aatgcttcca gcgcgttttc
    12181 tatcagattt cccaacgtgg taatcagcgt cgcgacctgg tcttcactgc cgctgtctgg
    12241 cagctggctt tcactgttta aaatcagcgt atggcctaaa tcggtcgcgc ggttaatctt
    12301 gctgattaaa aaaccagcta taaccggaga tttgatctta cccagcagag agccaatctc
    12361 ttcctgatag ttattggctg ttttgagaat gtaatcttcc aactgcttat aactcttcag
    12421 atgcaataat ccgagaatca cgtgcaattt attcataaat tcgtgggatc gttcacgaag
    12481 tgcgtcagca tagttgacca gaccgtcgag tcgctgcatc agtttacgta cttcagtttt
    12541 gtctctgaag gttgaaatgg caccgatgat aacgccatta ctgcgcaccg gaacggtgtt
    12601 gatcagtaat agccggtctt taatcgtaat ctcttcgtcg cggcgcgggg taccgtcgcg
    12661 taacacttcc gagacatcta ctacctgtga ccacgagtgg cttagcgtcg acagtttctc
    12721 atcgtcctgc gacttacggt aattcagcaa ttcttgtgcg gcatcgttga tcagcgtgac
    12781 ttcgccgcga tcgtccacgg caacgacgcc ttctttgata gactgcaaca tggcctggcg
    12841 ttgctcaaac agcgtggaga tttcgtaggg ttccaggcca aaaagaattt ttttcagtac
    12901 tttaaccaga atgcaggtgc cgatcagtcc gaccagcatg ccaaataata ccgaccagat
    12961 aatgctccag cgactgtcat tgatctgttg ggtcacgcga cttaactcaa ggccgatcgc
    13021 caccacgcca atttgtttat gattttcatc gtagatgggg gtaaatacgc gtaaagcctg
    13081 cgccagaaaa ccgcgattga tagcgacatt ttcttcgcca ttcagcgctt taaggatgtc
    13141 atcaccttta aatggctgac caatacgctg ggcttcagga tgcgagtagc gaagactttg
    13201 catatcggta acgacaataa acagcagatc gttgcgtttg cgcacggctt ccgcgatggc
    13261 ctggatgccg ctctcctgcg gttttttctg caagccctga cggatttccg gcgagtcggc
    13321 gagggtacgc gccaccgcca gtgccttgtt ggctagcccg tctcgcgtca tatcactgat
    13381 ttgcgagaag taaatcagat gcaccaccaa tagcaccgag aacagtaccg cgctgaccat
    13441 taagatcact gtggtactca atttcatcgg acgtttgcgt aacatgcggt agggcaatga
    13501 atgtctcatc agcttccttg tgtgacaaat ttcttaagca ttatctctga tgaggcgggt
    13561 aattcaaagg gagtaagaat gattggctat ataggggaag aggctcagga aactgaaact
    13621 gccggtgcgg tatgaagatt ccgggctatg cttattgcga aaaccatact gatgagaggg
    13681 ggaaggtcat ggatcaggat ctactggacg gtggttatcg ctgctatacc ggcgaaaaga
    13741 tcgatgtctg gttcaacact gcgatatgtc agcattctgg caattgcgta cgtggcaacg
    13801 gcaagttatt taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtt gacgtcacca
    13861 ctgtggttaa agtgattgat acgtgcccga gcggcgcgct gaaataccgt cataaataag
    13921 cgagggtaaa atggaaatac gcgaaggcca caataaattt tacattaatg acgaacaagg
    13981 caagcaaatc gctgaaattg tctttgtgcc gaccggagag aatttagcga ttatcgaaca
    14041 taccgatgtc gatgaaagcc tgaaagggca ggggattggt aaacagctgg ttgcgaaagt
    14101 cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca ctatgcccat ttgcgaaaca
    14161 cgaatttgat aaaacgcggg agtatgatga tattcgcagt tgatgggaga gtacagagtc
    14221 acgatatttt tcatgctctc cgcggtgtga tgcaggagag catctgaagg gtagggggat
    14281 gcacaaagaa tgggcagaga gagcgttttt ttgtcccaag tcatcccctt tactgagcaa
    14341 aaaaaaaaga atatctccta tatgagaatc atcgattggg gttaataagt tttgcgtccc
    14401 cagagcgttt aaaattgata ggagtcatat tatggaaggt aaaaacaagt tcaatactta
    14461 tgttgtttct tttgattatc catcatctta ttcctcagtg ttcttaaggt taagatcatt
    14521 gatgtatgat atgaatttct cctctatcgt ggctgatgaa tatgggatac cacgacaatt
    14581 gaatgaaaat tccttcgcaa taacgacatc attagccgca agtgaaatcg aagatttaat
    14641 caggctcaaa tgcttagact taccggatat tgattttgac ctcaacatta tgacagttga
    14701 tgactatttc cgtcagtttt acaagtagcc aggaaggata agaagaacat aaaaaatggc
    14761 actattctct aaaatattaa ttttttatgt gattggtgtg aacatatcct ttgtcattat
    14821 ctggtttatc tcacatgaga aaacacatat tcgtttactt agtgcattcc tggtcggaat
    14881 aacctggcca atgagtctgc ctgtggcatt acttttttct ctcttttagg agctgtagtt
    14941 gagtggaccc gctggttatc ttgaaacgaa gcgtctctcc tcaaaactgg atttgcggaa
    15001 cgacgaacga agcagtcgcc tgactgcttc gttcattaaa gtgaaattta tttctgtggg
    15061 cgcatcgccg ggaagagaat aacgtcgcga atagtatggc tgtttgtaaa cagcataatc
    15121 attcggtcga taccaatacc cagaccagcg gttggcggca gaccatattc cagcgcagtc
    15181 acgtaatctt cgtcatagaa catggcttcg tcgtcacctg cagctttagc gttaacctgt
    15241 tcctggaaac gttcagcctg atcttctgcg tcgtttaatt cgctaaaacc attaccgatt
    15301 tcacgaccac cgatgaagaa ttcaaaacgg tcggtgattt ccgggttaac atcattacgg
    15361 cgcgccagcg gggacacttc tgccggatat tccgtaataa aggttggctg aatcagatgt
    15421 gcttctgcca cttcatcaaa gatctccgtc acaatacgtc ccagccccca gcttttctct
    15481 accgtaattc cgatagattc agctaatgct ttagcggcat caaaattatc caggtcggcc
    15541 atatcggttt ctggacgata ttttttgatt gcttcgcgca tggtgagttt ttcaaacggt
    15601 ttgccgaaat caaacacatg ctcgccataa gtgactttag tggtacccag aacttcttgt
    15661 gccagggtgc ggaacaggga ctccgtcagt tcaatcaaat cgtggtaatc cgcatacgcc
    15721 atgtagagtt ccatcattgt gaactcagga ttatggcgaa cagaaatacc ttcattacgg
    15781 aagttacggt tgatttcgaa tatccgttca aaaccgccta caaccagacg tttcagatac
    15841 agctccggcg cgatacgcag gtacatatct aaatccagag cgttatgatg ggtaataaac
    15901 gggcgagcag atgccccacc tggaattacc tgcatcatcg gggtttctac ttccataaag
    15961 ccgcgcgcga ccatgaactg acggatagcg gccagaattt ttgaacggac aacaaacgtt
    16021 tgacgggatt tatcgttagc gatgaggtcc agataacgtt gacgataacg gacttcctga
    16081 tcctgcagac catggaattt atctggtaaa ggacgtagtg ctttagtcag caggcgcagc
    16141 tcagtacagt gaatggaaag ctcaccggtt tgcgtcttaa acagcgtacc gcgggcaccg
    16201 ataatgtcac ccagatccca ttttttaaac tgatcgttat aaacaccttc tggcaggcta
    16261 tctcttgcaa cgtacagttg aatacggcca ccgacatcct gcaacgttac aaaggaggct
    16321 ttccccatga tacgacgggt catcattcgg ccagcaaccg agacttcaat gtttaaggat
    16381 tccagttcct ggttatcctt cgcatcaaac tcttcgtgca actggtcaga ggtatggtcg
    16441 cggcgaaaat cattaggaaa cgccacacct tgctgacgca gtgccgccag tttttcgcgg
    16501 cgatttctca gttcatcgtt aaaatcaata gcctcattgg ctccccgtgt ttctcgttca
    16561 gacattttgg ttcctctaaa tccagctttc aatttagcgt agataaagag acagatcggt
    16621 acactgtatt atctgcctcg actattaata actcagttta tcttatcgaa actaacgctt
    16681 acctcaagtt gatatcacta ataaataacc atcaaaatgg tttaaatgac ttattaatct
    16741 cgatttatta gtgtgataaa ctgaaagtta atttattcat aactaattgt ttttacgaca
    16801 gttaatcgtt gctctcctgt atcatattcg ttggcgtgct aaacagaaaa cgggcagcaa
    16861 aggccaatac gacgatgatc gcgacacaag ccaacgtcca ctctcccatt tgagaaaaga
    16921 atcgctggta agcggcaatt gccataccgc taatttgcga ctctgtggtc tgctgtgcca
    16981 cgacgcctgc cagccagttg gcgaccgcgc ctgttgccag catataaata ccggttaata
    17041 cgccagacat tttcagacgc gtgatttgcg caatcgccac cgggtcaata aagagttcgg
    17101 caaagcccat tagcgccagc ccggatatca tcacgcccat tgacgcttga ccgtcagccg
    17161 ctgcatgtcg ggcatcaaat gccagcaaca taaagccaca agccatcagc aataagccaa
    17221 aggcaaactt cagccagacg cgcaatgttg agttgccgtg gctttctggc gacgccagcc
    17281 aggccagaac aaccccagcg agcatcaccg caattgcgtt cactgactgg aatagtgcag
    17341 taggtacttc aatattgaat gcctgacgat tcacaaagcg atcgataaac aagctgatgg
    17401 tactgccgcc ctgttgtgcc agtacccaga acaatgtccc gacaaacatc aacaatacaa
    17461 tttgccaaag agcacggcgg tgttcgggga atttgatcat catgcgggca atgatttgtg
    17521 cggcaatgag gcaaacgatc gccagcaaat atcctgacca gtcgttctcc agcagcaggg
    17581 taaaaaatac aggggctaaa cagagcatca ccactaacca gctccatact ggtaaggcaa
    17641 atttaacgct agtgagcgct tttttatcca tactacgcgt ggactggaaa tgacgatgac
    17701 cacttaagaa aatcaacaaa ccgataaaca tgccgccacc cgcaagggca aagccaacat
    17761 gccatccata ccactgagca gccaggccgc aggcgatggg agcggcgata gaaccgatat
    17821 tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg atgatcgttc tcgtcgtaga
    17881 gctcgccaag caaacagctg atgtttgatt tgaataaacc gtagccacaa ataatgattg
    17941 ccagcgccag atacaggcta aaagttgaat ttgtatcaat gcccagcacc acatggccaa
    18001 gggtcattaa cagcgcgccg gcaatcactg cagtgcggtt gccgagcagg cggtcggcaa
    18061 gccagccgcc gagaataggg gtaacgtaaa ccagagaagc atatgcgctg aacaggctga
    18121 tggcatggtt atcatcaaaa ccaagctgat gggtgagata gagaatgagt aaggcacgca
    18181 tgccgtaaaa actgaagtac tcccagattt ggatcgccac gatatagtat atcgcgcgcg
    18241 gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa aagggaagtg gcaagccact
    18301 tcccttgtac gagctaatta ttttttgctt tcttctttca ataccttaac ggtatagcgg
    18361 ccatcagcct gacggtatgc accgtgaata tcggtttcaa agcccggata gtgagcgccg
    18421 atttcacaca gcatctgcag gaactccaga accggacggc tttcttcggt gatcatttca
    18481 cccggcatta ccagaggaac tcccggcgga tacggaagga tcatattggc gttaatacga
    18541 cctaccattt cgtcgaggta aacttcttcg gtcataccgt gcagctcttt ctggaacgca
    18601 gcatacggag tcattaccat ggtcggcaga acttcaaatg cgcgatacat cagatccggc
    18661 agattgtggt gaacaatcag tttgtggata ttctgagcca gttcctgaat acgcatgttt
    18721 tcatagaatt caggatcttc acgatacaga gacggcagca tgtttttcac acgcaggttc
    18781 agatcgaacg cacgtttgaa gtctgtcaga gcacgcagca ggctcagtgc tttggtctta
    18841 tcgataccga tgctgaacag gaacagcagg ttatacggac cggttttctc aacaacgatg
    18901 ccatgttcgt cgaggtattt cgccacgatg ctggccggaa taccgaagtc gctcatggtg
    18961 ccgtcttttt ccatccccgg agtcagcagg gtgactttga tcgggtcaag atacatgtgc
    19021 tcgttatcga tgtttttgaa gccgtgccag ttgctgtcag aacgcagcgg ccagcattca
    19081 gtcgtatcga tatgatccgg ctgccataca tcaaagaacc agccatcaga ttccgttctc
    19141 agacgtttga tctctttacg gaatttgatc gcacgttcaa tggaaccgtt gatcagacgc
    19201 ttaccagcat tacctttcat catcgccgca gcggtttcag tggacgccac gataccgtag
    19261 tgcggagaag tggtggtgtg catcatgtag gcttcgttaa aggtttcttc gtttacgtca
    19321 cctttaacgt ggatcatgga agcctgagag aacgccgcca gcagtttgtg agtagactgg
    19381 gtttcgtaaa tcactttccc ttctacacga ccaccgctca taccgcattt accttcgtaa
    19441 atcggtgaga agttggtgta aggcacccac gcggagtcaa agtggatgga tttcacatcc
    19501 agtgttttct tgatgaagtc ggtattgtac agcagaccat cataggtaga gttggtaatt
    19561 acggcatgta ctggccaggt cgcgtttggt gtttctttca cgcgcttagc aatggtagcg
    19621 tgttggaatt cactctgtgg gataccacca agaataccgt aagcgttacg agtcgggcgg
    19681 aaatagattg gcgtaacatc gctcatcatc atcaggtggg tcagcgattt gtggcagtta
    19741 cggtcaatca gaatggtgct gcctgccgga gcagagtaca taccaacaat tttgttcgca
    19801 gtggaagtac cgttggtcac catgtagctg cggtctgcgt taaatacgcg agcgatatac
    19861 tgttctgctt ctttgtgtgg accactgtga tccagcagag aacccagttc agatactgaa
    19921 atggaaatat cagatttcat ggtgttcgga ccaaagaaat catagaacag gctacctacc
    19981 gggcttttct ggaatgcagt accgcccatg tgaccaggag tacagaaagt atatttacct
    20041 tcacgaacat atttaaacag tgctttagtc agcggaggca gaatagtgtt gatatattcg
    20101 tcagtggtct gcttgatctt gttagcaata tcttcagcag cacccagcgc atattcaaag
    20161 aagctaatct gtaaacgcag gtcattcagg cttacatcga gagtggaata cgtattagcg
    20221 aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt cttcgcacag ctcgagatta
    20281 tatttatccc agtcaaaaat aacgccgcac agacgcgcat tgttttcgat cagttttaat
    20341 aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca gacgttcaag cgcgcgatga
    20401 agttcacgga tgggttcttc tttaaaataa acccccatgt gattcaatat tgcaataacg
    20461 ttcatagtca tatctccagg taaaaaaggc ccctcccaac acatgggaca aaatgaaagg
    20521 aggagcctcg gaaaatactt ttaattaatg tgcgttagac gctgtgtggt tatccattga
    20581 gtggctctgg cgctcatgca ttttgcgagc gtagaacatc aggataatca ggctgacgat
    20641 gaaggtacct gccagctcga aggagcttgc gcccatcagc gcgatgaagc agaacacgca
    20701 acccagtaca gagcagatca ggctgacaaa gttgcggatg ttaacgcctt caaaacgaat
    20761 caggtcaacg caagagtaga agtacggcag catagtcagc agtactgcga taccggtcag
    20821 ttcaccgaac aggtcagatg ctttaccacc ggcagagttc atcagagtga tgaggatcat
    20881 cagggcagtc attttcactg cagccaacag cagacctttt ttcggaatac cgttgctgtc
    20941 gacttcacca taaactttcg ggaagttacc gtcgttagcg gcacgtacac ctgcctggcc
    21001 taccaacatc atccaggagc ccagagaagt caggcacgca aaggcggtga atgcagaaac
    21061 cagcggcgca gcccagttac cgaggatagt tgaagcactg attgcaaacg gagcaccgga
    21121 agccgccatt acagaagacg gatacatacc ggaaagcacc tgagtcgcag cgatgtaaac
    21181 aatacctgct aaaccagtac ccagcatggt tgccagcgga acggtacgtt tcgggttttt
    21241 aaccatacca gtacttacag ctgcggattc aacacccacg aaggcccaca ggcagagcag
    21301 aatactttta atgatcgcat gaccatcagt ggtatccgca gtattccagt tagcagcata
    21361 agttgccgca tcaaaccaat gccagccaac aatagctgtc attaccacag gaataagaac
    21421 cagcaccaga ccaatagtgg ttaaacggct tacccaggta ccgccgagca tatttacaaa
    21481 ggtaaatacc cagacgatag caatacaggc gatacccgcc ggaacaggat catttaatac
    21541 tgggaagaag gtggaaagat aagatacagc ggtaatacca atcgccaggt taccaatcca
    21601 gttagcatgg taataaagaa cacctgtctg aaaaccaaat gcaggggaaa tttctccggc
    21661 ataagcaatt gggccacctt gttgcgggtt ttttgttgcc agtcgggcat atacatacgc
    21721 cagcgacatt gcaccaataa tagagataat ccaaccccag atagcaatac caccgatact
    21781 tgctaggttc gcaggtaata atgcaatacc gctccccatc atattaccgg caacaacacc
    21841 ggtacaggca aatagcccga tcttcttggc agaactcatg ctcttctcct aatttcattt
    21901 ttgaatttgg agtccgggtc atgatgtata actatttcct gaccagacca aactggcgct
    21961 aagattactc acgaaaaaag gattaatcct aaagattagg tgaaataaca caaaagtttc
    22021 tgtaagtgag aacttgaggt tttttattaa cacatcagga tcgcaagttg atatcatgaa
    22081 aagataaaca ttcaatgttt acaataaatt gcgggacatt atctggttaa atttatgtaa
    22141 taaaaattat gcggcaaata aattgccgca acatattata ccaacatgaa catacaaaaa
    22201 atcaacaaca aatattcccg agtatgaatc aacccgggag tcacttattc tgaagcgaga
    22261 aatttgtcga gataaggtac aacataagga acagaagtct ggaatatacc attttcaatc
    22321 cagtaaaggg tgtttgcccc tgggcgtaaa ttaaaagcgg tgagatatgc atcagctgct
    22381 tcccggttca tccccttcat ttcataaacc ttgccaagca acacataatt tagccaggac
    22441 atttcaagat caatgccagt atttatcgcc tggtaagact catctgtttt accttttacc
    22501 agagcactga ccgcttttat ttgatatata atggacaggt tattcaattc cggcagtgta
    22561 acaatgttat ctatttctgt attcagtgct gctaattgtt tttcatctaa aggatgttga
    22621 gaatggcgca cgatatcaac taatgctttt tctgctctcg cgtaggtaaa ttctggggat
    22681 gattgaacaa tctcacccaa taattcactg gcacggttca atgatttatc atcgccatgc
    22741 agtaaataat catgtgcctg ataaaaatta gttaataacg caccacgatg aggcaaaatt
    22801 tgctggagca tctcctgcat tcgttgtggc cacggttggt ttaacgcttt tgataaactc
    22861 tccagtaaat cattttgaat cgccagctga ttaccgttag tgatgacata acgtttatcc
    22921 agcatggttg aaccatctgc attgtctacc aattttaccg acataaagca ttgttgagca
    22981 cggtattggc gctgattaac aaacgcaata gataatgttt taccggaact gctcggttca
    23041 tcaatgttgt agttgatttt gtcatgcacc ataaaggtgg agaaggtgtt aagtgatgtc
    23101 gccaccaaat cacccacgcc tatcgcgtaa gagagctgat acggggaact ccagctgtta
    23161 caacttttat ttaccatatt aatgtcaata tcgcgtggat tgagcaaaat acgcgatttg
    23221 ctcatcggaa gacgtgtatc aagacttgaa aacgctacca gtgctacaca gatacctaac
    23281 gacaacagga aaaaaaacca tacccaaaag gtagtgaatc gtttgctttt aactggggat
    23341 tgttcaggtg gcgttgcggt gttttgaatg ttaagactgt gggagggaga atctgtggca
    23401 ggaaccgcct ctggtatagg gggaggcgaa gatagcatta tttcctctcc ctcttcttcg
    23461 ctgtaccaga taaccggcac cattaattta tagccgcgct ttggtacagt agcgatatag
    23521 acaggactat cttcatcatt atcttttaat gacttacgta gttctgagat actctgcgtc
    23581 acaacgtgat tggtgacaat acttctcttc cagacattat cgataagttc atccctgcta
    23641 agtacttcgc cactgtgttg agcaaagaaa accagaagat cgattaatct cggctcaagg
    23701 gtaagttgac gcccattgcg gctaatttgg tttatggacg gagtaacaag ccattcgcca
    23761 acgcgaacta caggttgttg cataatagaa actcattcga aaagggaatg atgcaatgat
    23821 aattgccaca acctattttt accatctata gatggtttta tttacatatt attggtgaat
    23881 gcaagacgtt atttttacca gccataaact tcttgattac atggtattac gaaaggattt
    23941 tactgagaac cagaagtaat attccttatc atcaaaattc atcatctttg ccaaagaaaa
    24001 atgttcataa aataatccat gggaaattgc ccggcgcgct tactattttt atggattgtt
    24061 agtctttgca tgagcaagcg gactgataca tttctctttg ttctcattca gaaaatctca
    24121 tcagtcggcg ttctgcccga ggttgtgctt tatggatgcg ttacgctcct gatgacgtca
    24181 tctgacgttc aacagcatca cggggccgcg cgacatttca cgtcagtcag tgctatcgct
    24241 caggaacaca tttccccgaa ttgggatatg cccgcaaatt gctggtgatg tggggagaat
    24301 ctggttgagt tcggtagaaa tgatttttag gcagaacgct taaattgtgg cgt
//
LOCUS       DAOTRK010000068        24039 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.068, whole genome shotgun sequence.
ACCESSION   DAOTRK010000068 DAOTRK010000000
VERSION     DAOTRK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.068"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            28..546
                     /locus_tag="QIE41_002571"
     CDS             28..546
                     /locus_tag="QIE41_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDS3531448.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            562..1101
                     /locus_tag="QIE41_002572"
     CDS             562..1101
                     /locus_tag="QIE41_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDS3531449.1"
                     /translation="MKKVFLCAILTSLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1196..2773)
                     /gene="guaA"
                     /locus_tag="QIE41_002573"
     CDS             complement(1196..2773)
                     /gene="guaA"
                     /locus_tag="QIE41_002573"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDS3531450.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     TRIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2842..4308)
                     /gene="guaB"
                     /locus_tag="QIE41_002574"
     CDS             complement(2842..4308)
                     /gene="guaB"
                     /locus_tag="QIE41_002574"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDS3531451.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMFKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4470..5840
                     /gene="xseA"
                     /locus_tag="QIE41_002575"
     CDS             4470..5840
                     /gene="xseA"
                     /locus_tag="QIE41_002575"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDS3531452.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     AQQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5837..6052)
                     /locus_tag="QIE41_002576"
     CDS             complement(5837..6052)
                     /locus_tag="QIE41_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDS3531453.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6122..7594)
                     /gene="der"
                     /locus_tag="QIE41_002577"
     CDS             complement(6122..7594)
                     /gene="der"
                     /locus_tag="QIE41_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDS3531454.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7712..8890)
                     /gene="bamB"
                     /locus_tag="QIE41_002578"
     CDS             complement(7712..8890)
                     /gene="bamB"
                     /locus_tag="QIE41_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDS3531455.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8901..9521)
                     /gene="yfgM"
                     /locus_tag="QIE41_002579"
     CDS             complement(8901..9521)
                     /gene="yfgM"
                     /locus_tag="QIE41_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDS3531456.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9539..10813)
                     /gene="hisS"
                     /locus_tag="QIE41_002580"
     CDS             complement(9539..10813)
                     /gene="hisS"
                     /locus_tag="QIE41_002580"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDS3531457.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNCTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10924..12042)
                     /gene="ispG"
                     /locus_tag="QIE41_002581"
                     /gene_synonym="gcpE"
     CDS             complement(10924..12042)
                     /gene="ispG"
                     /locus_tag="QIE41_002581"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDS3531458.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFEQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12069..13082)
                     /gene="rodZ"
                     /locus_tag="QIE41_002582"
     CDS             complement(12069..13082)
                     /gene="rodZ"
                     /locus_tag="QIE41_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDS3531459.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13367..14521)
                     /gene="trmG/rlmN"
                     /locus_tag="QIE41_002583"
     CDS             complement(13367..14521)
                     /gene="trmG/rlmN"
                     /locus_tag="QIE41_002583"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDS3531460.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14671..15102)
                     /gene="ndk"
                     /locus_tag="QIE41_002584"
     CDS             complement(14671..15102)
                     /gene="ndk"
                     /locus_tag="QIE41_002584"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDS3531461.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15251..17563)
                     /gene="pbpC"
                     /locus_tag="QIE41_002585"
     CDS             complement(15251..17563)
                     /gene="pbpC"
                     /locus_tag="QIE41_002585"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDS3531462.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPTNLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLMDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            complement(17564..22525)
                     /gene="yfhM"
                     /locus_tag="QIE41_002586"
     CDS             complement(17564..22525)
                     /gene="yfhM"
                     /locus_tag="QIE41_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDS3531463.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSEKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIQVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSVSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGVSRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDVINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGQSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22732..23577
                     /gene="sseA"
                     /locus_tag="QIE41_002587"
     CDS             22732..23577
                     /gene="sseA"
                     /locus_tag="QIE41_002587"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDS3531464.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNATFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGAFNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
BASE COUNT         5753 a         6643 c         5984 g         5659 t
ORIGIN      
        1 ttttagtaat gtgtataaca aggaatagtg atgaaattta aaaaatgtct tctgcctgtg
       61 gcaatgttag cgtcattcac tctggcagga tgccagtcaa atgctgatga tcatgccgcc
      121 gatgtttatc aaaccgatca actgaatacc aaacaagaaa ctaaaaccgt taatattatt
      181 tccattcttc ccgcaaaagt tgccgtagac aactcccaaa ataaacggaa cgcacaagcc
      241 ttcggcgcgc ttattggtgc agtcgctggc ggtgtaatcg gccacaacgt ggggtctggc
      301 agcaattccg gaacgacggc aggtgcagtt ggcggcggag ctgttggcgc ggcagcgggt
      361 tctatggtga atgataaaac cttagtggaa ggtgtttctc taacgtataa ggaaggcacc
      421 aaagtgtata cctctaccca ggtgggtaaa gagtgccagt ttacgacagg tttagccgtt
      481 gttattacca cgacgtataa cgaaacgcgt attcagccaa atacgaaatg tcctgaaaag
      541 agctaatgat caggaggagt catgaagaaa gtttttcttt gcgccatctt aacctcctta
      601 agctatccgg ctatcgcctc atcattgcag gatcaactct ctgctgtcgc agaagcggaa
      661 cagcaaggta aaaatgaaga gcaaaggcag catgacgaat gggtcgcgga gcgcaacagg
      721 gaaatccagc aagagaagca acgtcgcgca aacgcccagg ccgccgctaa caaaagagcg
      781 gcaacggcag cggcaaataa gaaagctcgt caggataaac tggacgccga agccactgcg
      841 gacaaaaaac gcgatcaaag ttatgaagat gagctacgca gcttagagat tcagaaacaa
      901 aaactggcgc tggcgaaaga agaagcccgc gttaagcgag aaaacgaatt tatcgatcag
      961 gaactgaagc acaaagctgc gcaaaccgat gtggtgcaat ctgaagctga cgcaaacagg
     1021 aatatgactg agggcggtcg cgatctgatg aaaagcgtgg gcaaagcaga agagaacaaa
     1081 tcggacagct ggtttaatta atcgatgtaa gtaacttcaa gcctataatt cttgaagata
     1141 aaaaaccctc tgtagtaaca gagggttttg ttcattcata gtgcagggat caaaatcatt
     1201 cccactcaat ggtagctggt ggcttgccgc tgatgtcata caccacgcgg gaaataccgt
     1261 tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa atcgtacggc agatgcgccc
     1321 agtgtgcggt cataaagtcg atggtttcga cagcacgcag agaaacaacc cagtcatact
     1381 tacgaccatc gcccattacg ccaacggaac gtaccggcag gaagacggtg aatgcctggc
     1441 tgactttgtc gtacaggtcc gctttacgca gttcttcaat gaagatggcg tcagcacggc
     1501 gcagcaggtc acaatactct ttcttcactt cacccagaac acgaacgcca aggcctggtc
     1561 ccgggaacgg gtgacggtac agcatgtcgt acggcaggcc cagctccaga ccaatcttac
     1621 gcacttcgtc tttgaacagc tctttcagcg gttcaaccag gcccatcttc atctctttcg
     1681 gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc tttaccggtt gcagacgccg
     1741 cagattcgat aacgtcaggg tagatagtac cctgcgccag ccacttcacg tcttccagtt
     1801 tcagcgcttc ttcatcgaat acttcaacga aaacgcgccc gatgatttta cgttttgctt
     1861 ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc ttctgccgga acgtgaacaa
     1921 tattgagacc aaagtgatcg ccaaacatat ccagaacctg ctctgcttcg ttgagacgca
     1981 gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc gatagcgcgg tgcagcagca
     2041 ttgcggttac ggaggaatcc acaccaccag agaggccgag gatgacttta tcgtcgccta
     2101 cctgctcgcg gatgcgagtt acagcatcgt cgataatttt cgctggcgtc cacagggctt
     2161 cacactggca gatatcacgc acaaaacgct ccagcatgcg cataccctgg cgggtgtgag
     2221 tcacttccgg gtggaactgt acgccataga agcgtttttc ttcgttagcc ataatggcaa
     2281 acgggcagct ttcggtgctg gctacggtga cgaagtcgga cgggatagcg gtaactttat
     2341 cgccgtggct catccagaca tcgagcagag gtttaccgtc tgcggtcaga gcatcttcaa
     2401 taccgcgaac cagtgcgctg tcgtttacga cttcaacctg cgcgtagcca aattcacgtt
     2461 cgttagaggc ttcaacgtga ccgcccaact gcattgccat ggtctgcatg ccatagcaaa
     2521 cgccgaatac cggtacgcct gcttcaaaga catactgcgg cgcacgcggg ctgttttctt
     2581 cggtggtgct ttccgggccg ccggaaagaa taatgccgct tggattgaag tcacgaattt
     2641 gtgcttctgt cacatcccac gcccacagtt cgcagtaaac gcccagctca cgcacgcggc
     2701 gcgcaaccag ttgagtatac tgagaaccga agtccagaat gaggatgcga tgcttatgaa
     2761 tgttttccgt cattgacgct tattccgagg caagtgaaac agataatata aatcgcccga
     2821 catgaagtcg ggcaaagaga atcaggagcc cagacggtag ttcggggact ctttagtaat
     2881 ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg ctgatacgta caaactccgc
     2941 tttagtacgc agttcgtcga tagtaccaca gccggtcagc cccatacagg agcgcaggcc
     3001 gcccatctgc tggtgaatga tctctttcag gcgaccttta taggctacgc gaccttcgat
     3061 accttccggc accagtttgt cggcagcgtt gtcgctctgg aagtaacggt cagaagaacc
     3121 tttgaacatc gcgcccaggg aacccatccc acggtaagat ttgtaagaac ggccctggta
     3181 gagttcgatt tcacccggag attcttcagt acccgccagc atggaaccca ccattaccgc
     3241 gcttgcgcca gcggcgatag ctttggcgat atcgcctgag aaacggatac cgccgtcagc
     3301 gataaccgga ataccggtgc cttccagggc ttctactgca tcagcaacgg cggtgatctg
     3361 tggaacaccg acgccagtca cgatacgagt cgtacagata gagcctgggc cgataccgac
     3421 tttaaccgcg ctgcaaccag cttctgccag agcgcgtgca cctgcagctg ttgccacgtt
     3481 gccgccgatg atttgcagat caggatattt agcacgggtt tcacggatac gttgcagaac
     3541 gccttctgag tgaccgtgag aggagtcgat cagcagaacg tcaacgcctg cggcaaccag
     3601 cgcgtcaaca cgctcttcgt tacccgcacc cgcgccaacc gctgcaccaa cacgcagacg
     3661 gccttgctcg tctttacagg cgttcggttt acgttccgct ttctggaagt ctttcacggt
     3721 gatcatgccg atcaggtgga attcgtcatc aaccaccagc gccttttcaa cgcgtttttc
     3781 gtgcattttt gccagcacca cttcacgggc ttcgccttca cgcacggtta ccagacgctc
     3841 tttcggcgtc atgtaaacgc taaccggttg attcagatcg gtcacaaaac gcacgtcacg
     3901 accggtgatg atgccgacca gttcgttttc ttcggtaacc accgggtagc ccgcaaagcc
     3961 gttacgctcg gtcagttctt tcacttcacg cagggtggtt gtcggcagca cggtctgcgg
     4021 atcagttacc acgccagatt cgtgtttttt cacacggcga acttcttctg cctggcgttc
     4081 aatggacatg tttttgtgga taaagccgat accgccttcc tgagccagag caatagccag
     4141 gcgcgcttcc gttacggtat ccattgctgc ggaaagcata gggatattca gacgaatagt
     4201 tttcgtcagc tgggtgctga ggtcagcagt attcggcaga acggtagaat gagcaggaac
     4261 gaggagaacg tcgtcaaacg tcagagcttc tttagcgata cgtagcatgg gcaatatctc
     4321 gaccagagtg gttaataaat attgccgcgg cattatacag agcgtaaccg attgcatcta
     4381 cccctttttg caaaaaatgc ttgctatccc cggagggcgg gttactatcg actgaataac
     4441 ctgctgattt agaatttgat ctcgctcaca tgttaccttc tcaatcccct gcaattttta
     4501 ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga gcatgagatg ggacaggttt
     4561 ggatcagcgg cgaaatctct aatttcacac aaccggcttc cggtcactgg tactttacac
     4621 tcaaagacga caccgcccag gtacgctgcg cgatgttccg caacagcaac cgccgggtga
     4681 ccttccgccc acagcatggg caacaggttt tagttcgcgc caatattacg ctctacgagc
     4741 cgcgcggtga ctaccagatt atcgtcgaaa gtatgcagcc tgccggtgaa gggttgctgc
     4801 aacaaaaata cgaacagctt aaagcgaaat tgcaggctga aggtctgttc gatcagcaat
     4861 acaaaaaatc actcccctct cctgcgcatt gcgttggtgt gatcacctca aaaaccggtg
     4921 ctgcgctaca tgatattttg catgtattaa aacgtcgcga tccttctctg ccggtgatca
     4981 tctaccccac cgccgttcag ggcgatgacg cgccagggca aattgttcgc gccattgaac
     5041 tggcgaatca gcgcaacgag tgcgacgtgt tgatcgttgg gcgcggcggc ggttcgctgg
     5101 aagatttatg gagttttaac gacgaacgtg tagcgcgggc gatttttgcc agccgcattc
     5161 cggtcgtcag cgccgtcggg catgagacgg atgtgacgat tgccgacttt gtcgctgatc
     5221 tgcgtgcgcc aacgccgtct gccgccgctg aagtagtgag ccgcaatcaa caggagttac
     5281 tgcgtcaggt gcagtctgcg cagcagcgcc tcgaaatggc aatggattat tatctcgcca
     5341 accgcacgcg tcgttttacg cagatccatc atcgcttaca gcagcagcat ccacagctcc
     5401 ggctggcacg ccagcaaacc atgcttgagc gcctgcaaaa gcggatgagc tttgcgctgg
     5461 aaaatcagct taagcgtgcc gggcaacagc agcagcggtt aacacagcgg ctaaaccagc
     5521 aaaatccgca gccgcgtatt caccgcgcac agacgcgaat tcaacaactg gaatatcgcc
     5581 tggcagaaac gttgcgcgcg caactgagcg caacgcgaga acggtttggt aatgcagtaa
     5641 cgcacctgga agcagttagc ccgctttcga cgctggcgcg tggatacagc gttactactg
     5701 ctactgacgg caatgtactg aaaaaagtga agcaagttaa agcgggtgaa atgctaacca
     5761 cacgtctgga agacggctgg atagaaagtg aagtaaaaaa catccagccg gtaaaaaaat
     5821 cgcgtaaaaa ggttcattaa gccagcacga attcaacgcg ttttttcgat atcaagccat
     5881 gcccgtgctg acagaagtaa tctactgcac cgcaggcttt taacacctgc aacggttgat
     5941 ggcagtctgg acaaagagct ttcatttcaa taaattcccc gcaggatggg caacgcgcat
     6001 ggccgttatc ctgatcaagc acatgctgac attgtggaca atgcagttcc atatgaattt
     6061 ctccggtagc cattccctct acattcatag agggaatggc agataaaata cttacggata
     6121 attatttatt tttcttgatg tgcttcatca gacgcttacg tttacgcatc tgggttggcg
     6181 tcagggtgtt acgcttgttc gcatacgggt tttccccttc tttgaactga atacgaatcg
     6241 gcgatcccat tacgtccagc gatttgcgga agtagttcat caagtaacgc ttgtaggaat
     6301 caggcaggtc tttcacctga ttaccgtgaa tcaccacaat cggcgggtta taaccaccgg
     6361 cgtgggcata tttcagcttc acacgacgac cgcgtaccaa cggcggttgg tgatcttcaa
     6421 cagccatcgt catgatgcgc gtcagcatag aggtccccac acgacgggtg gagctgtcat
     6481 acgcttcacg tactgattca aacagattac caacaccact gccgtgcaag gctgagataa
     6541 aatgcacacg agcaaaatcg ataaagccca gacggaagtc cagcgtttct ttcacctgct
     6601 ctttcacttc ctggctcagg ccatcccact tattcaccac aatgacaagt gagcgcccac
     6661 tattgagaat aaagcccagc agggagagat cctgatcgga aataccttcg cgcgcatcaa
     6721 tcactaacat caccacgttg gcatcttcaa tggcctgcaa cgttttgatt accgagaatt
     6781 tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc agcggtgtca atgagcacat
     6841 actcacgtcc atcgcgttcc attggaatgt agatactgtc acgcgtcgta ccaggcatat
     6901 cataaacaac aacgcgctct tcaccaagaa tacggttagt gagtgtagac ttacctacgt
     6961 tcggacgacc cacaatcgcc agtttgatcg gcagactttg cgggtcgaag tcgtcttcct
     7021 cttcttcttc gccgttctct tccgcttcaa attgcgccca gtattcggcg tcttcgtcga
     7081 cttcttcttg cggtgcgaga tcttccatcc acggcagcag cacgtgctcc agcagactta
     7141 atacgccacg accgtgagac gcggcgatcg ggtagatttc ccctaaacca agcgagtaga
     7201 aatcaaccac cgcctgatcg ggatccagac cgtcagtttt gtttgccacc aggaaggtcg
     7261 gtttttcacg ggagcgcaga tgtttggcaa tcgcttcatc cgccggcatc aggcccgcgc
     7321 gcgcatccac cataaacagt acgacgtccg cttcttcaat cgccagcagc gactgttccg
     7381 ccatacgtgt ctctacaccg tcttcggtgc catcaatccc accagtatca atacaaataa
     7441 actcgcggcc ttcaatttct gcacgaccgt acttacggtc acgagtcaga cccgggaaat
     7501 ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa taacgtggat tttcctacgt
     7561 tagggcgccc gacaagcgcg accacaggta ccatgtttaa agcctcattt ttataaatca
     7621 tcagacaacg cacgctatat tcgtgtcgtt gttaaaaaca ggaaaacggc ccctgtccag
     7681 gagccgtttt caaagtgaac gacagagacg attaacgtgt aatagagtac acggttccgt
     7741 cttttgcctg gatcagcagt ttgccatcag cggcaaccgg ttcagtctgg aaaccggaac
     7801 tatcaacttt ttgctgggca acgaaacgac catcttcgac gttaatccag tgcagataac
     7861 cttcgctgtc acctaccacc aggttgccat tatacagcac cggagaagtc agcaggcgat
     7921 gcaacagatc gctttgtgtc cacagcgtaa cgccgccatc aatggtcaac gccatcaccc
     7981 ggtcattttg atcgaccaga tagatgcgat tgccgtcgac gatgaaatca ttcaccgaac
     8041 ccagttcgcg tttccacata atctgaccac tgcgcagatc aagcgccgtc aggttaccat
     8101 tataggccag cgcgaaaaca acgccgttaa caacgacggg agtcgtgtca acatcgctca
     8161 gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg ctgctgccaa atcatctggc
     8221 cctgttccat cagcactgcg ctgacgcgac cattatcgcc acccacgacg gccgcaccaa
     8281 aagccgttgc cggcgcagac tcgccacgca aagagagcga aggcatatcg aggttaactg
     8341 tccatttgac agcgccgtca gcttcgttca gcgcttgtaa ctgaccgtta ctggtgtgga
     8401 ttaacaccag accgtcgctg accaccggac gcgaaagtgc ttcacccgcg actttagttt
     8461 gccatgccac agtaccatcg ctggtattca gcgcgtaaac ctgcgccttt tcgctgccaa
     8521 tgtagacatg cccaccagac acggtcacac cgccagaaag taatgcaggc tctttagaga
     8581 accagccatc tttctcggcc aggctgacag accagatttc tttgccatca tccgcattca
     8641 gcgcttttac taaaccagcg cggtccgctg catagacaac gttgtccgcc agtgccggat
     8701 gaagattgga atagaagttg ccaatgccgc taccaacgga agtgctccac gccgtggtcg
     8761 gcgtaaactg gttttcaacg gtcggcaatg gggacatctt taccacatct tcttcgctgt
     8821 taaacagcga acagccgctt aaaagggtaa cggaaagcag tcctggcagc agtaatttac
     8881 gcaattgcat cgggtccctc tcagatggac aaattattaa ttttcatctg catcatttcg
     8941 ctcagtgccg gggtaacatc gcttttcacg cctgcttccc atgcactacg cgcaccttgc
     9001 ttatcacctt tgctcagcaa tgcttcacca cgcaggtcgg caacaatggc agcccaccct
     9061 tcacctttga tggtatcaag ggttttcagc gcggcatcag cctgcttgag ctgaacctga
     9121 acgcgagcaa gacgcagatt tatcacggct ttgagatttt catcgctcgt gtctgccagc
     9181 ccctgttgta actgggcggc agctttctct agttcatttt tgtcaacaaa ttgctgcgca
     9241 agttccaaag aagccagcgc accataagta tttttatttt cagcagcaaa tttttccgcc
     9301 gccggaatgc tatccggttt gccttcgctc actgcggtaa ccgcattttg ataggcaaga
     9361 gaagcggagc gtgcagaatc aacctgatgg ctgttccagt agcgccagcc aattagtgcg
     9421 ccaacgccca aaatcacccc aacagccagt gctttgccat tttcagcaaa aaagcgttta
     9481 accgcttcta cctggtcgtt ttcgttctcg taaatttcca cgctgtcctt ctccttcctt
     9541 aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg cgcaactgcc gtttgctcac
     9601 cagagcgcaa atccttcact actgctgtgc cgttagccac ttcagactca cccagcacca
     9661 cagcaacgcg ggcaccccat ttatcagcac gggcaaactg tttcttaaag ttgccgccgc
     9721 cgtggttggt catcaatttc acgcccggta attcatcacg cagacgctct gctaatgcca
     9781 tagccgcaga ttgtgtatca gcacctgaag ccaccaggta tatatcgaca acaggatcgg
     9841 ccttaaattc cggattaacg gcctgaacta acaatacaag acgttcgagg cccatcgcaa
     9901 aaccgacagc cggtgtagca cgaccaccca gttgttccac cagaccgtca taacgaccgc
     9961 ctgcacacac ggtgccctgg gaaccgaggc tgttagtcac ccactcgaaa acggtacagt
    10021 tatagtaatc caggccacgc accagacgct ggttgacggt gtaagcgatc cccgcgctct
    10081 caagcagttt gcacagaccg gcaaaatgct cacgagattc ctcgtccaga taatcgccta
    10141 atgccggagc gtcgttgaga agcgcctgca cttccggatt ttttgaatcc agcacgcgca
    10201 gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag cttttcttta tgctgctcaa
    10261 ggaatgccac cagcgcatcg cggtaattcg cacgagcttc cagcgaaccg atagagttca
    10321 gctcaagtgt tacgtgctcg gaaataccca gcgcgcgcca ccagcgggca gtgagcataa
    10381 tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac ttcgcagccc aactgatgga
    10441 actgacgata acgccctttc tgcggacgct cgtgacggaa catcggcccg atataccaca
    10501 gacgctgttc ctgattgtac agaagaccat gctcgatgcc ggcgcgtaca cagcccgccg
    10561 tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc ctcaaaggtg tacatctctt
    10621 tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa tagcggggtc tgctctacaa
    10681 tcggcaagcg gatttcactg taaccgtagc tgccgagcac gtttttcagt gtgccttcaa
    10741 tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt catgccgcga atggcttgaa
    10801 tgttttttgc cacgtttatt ctctttctga atataaaaat gaaccctcaa cgcttccctc
    10861 aatgtttcgg gagccatgcg ggttcaatca tacacgggaa gcgaggcgct tcccatcacg
    10921 ttattatttt tcaacctgct gaacgtcaat tcgacgcgct tcgtccagct gactggcttt
    10981 cgcacgaatg cgcgcttcca gctggtcgat catatcgttg ttgtccagac ggtctttgcg
    11041 cacgccatct tcatagaggc cgcttttctt gttaccgccg gtgacgccga gtgtagaaac
    11101 cagcgcctca cctgggccat tcaccacaca gccgataatc gaaacgtcca tcggagtgat
    11161 gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg ataacatcaa actcctgacg
    11221 cgaacaggtt ggacaggcga tgaagttgat cccgcgcgaa cggatacgca gcgatttcaa
    11281 aatatcgaaa ccgactttga tctcttcaac cggatcggcc gccagcgata cgcgcagcgt
    11341 gtcgccaatg ccttcagaca gcagcagacc taaaccaatg gcggatttaa ccgccccgct
    11401 gcgcgcacca cccgcttcgg tgatccccag atgcagcggc tgatcgatct gttttgccag
    11461 caaacgataa gactcaacag cgaggaagac gtctgacgct ttcacgctga ctttgaactg
    11521 ttcgaagttc aagcgatcga gatgatcaac atgacgcatg gcggattcca gcaacgcctg
    11581 cggcgtcggt tcgccatact tttcttgcag atctttttcc agcgatccgg cgttaacgcc
    11641 aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc accatgcgaa tacgctcttc
    11701 attaccgata ttgccagggt taatacgcag acaatcgacg ccgtattccg ctactttcag
    11761 cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc acgttaacct gctgtttgat
    11821 gagtttgaac gcttctgccg cgtccatcgt cggtactgag acacggacga tatcagcgcc
    11881 aacacgttcc aacgccttga tttgattgac cgttgcttcg acgtctgtcg tacgcgtatt
    11941 ggtcatggac tgtacggcga tgggagcacc atcgccaatc ggcacattcc caacgtaaat
    12001 acgtgttgat tttctacgtt gaattggagc ctggttatgc atgaaaaatc tcccgcgtta
    12061 cccgtctgtt actgcgccgg tgattgttcg gcattgaggg tcagacgcgc aacctggtta
    12121 gttctgataa aacgactcag atcgacaggt ttcccttgat actggatctg tactgcggct
    12181 ggcgcaccaa ttttcagttt atacggtgcc tggccggtta aattcaaatt accgtcttta
    12241 cgctgcatcc cgctaaacag ttttttaccg gtagcatcag tgacctccag ccagcaatcg
    12301 gcagtaaagt tcatcaccag cgcattcgga tcagccgccg gcgtggtcac gccagcctga
    12361 tcggttggca acggcgcagc accatctggc gttgttgttg ccgttggtgc cggggtcgcg
    12421 gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat tctgttgcgg atcaacagct
    12481 ggtgctggcg cagttacggc aggtgtttgc gtgttggttg cggtagtatc cacagacgct
    12541 ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg tatttaacgg aacactctgc
    12601 ccctgctcgc tattactgct caattccgcc gaagattgat cggccatagt ggtgatctct
    12661 tcctgctgag ctttgtggtc ttgccaccac caggcaccgc tcaggccgat aaccacaaac
    12721 aacaccagcc aggtgaaggt catcagccag ccgtcgcgtt ttttgcggcg tttaccgagg
    12781 gaaaaactct gcatcggcgc aacttttgca gcccgaagtg gagcctgctt ttccagccct
    12841 ggcagcagtt cttcttctgg aatatgtacc agacgcgcat aagagcggat atatccgcgc
    12901 aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt caatgtcgcg taccgtggaa
    12961 accttcaggc aaagtcgctc ggcaacggcc tgctgactaa gtcctagttg ttcgcgagca
    13021 ttacgcaggc gagcgccggt agtaagtgct tcattttggt cgtgcgtggc ttcagtattc
    13081 attcgctgca ggtacgttta aatgagaatt gagatgccgg tgaaccatca tgctcaccca
    13141 caccgcgaaa catccgttaa gttaaccgtt atcatacagt ataagaccgt ctgtgtgctc
    13201 gtgacaaagc ccacattcac taaggctaac ttactgttgc atcgttacat actgccttaa
    13261 agtcagcaaa aacgcaccgt taatattgac cagacaattg caacttaata cttcattcat
    13321 aaaagattac gccacggtac ataaagtaac cgtggcgtaa tggctatcag accgctttaa
    13381 tatcgatggc ttcaccctgc atccgtttac gcagggtacg tttcgtacgg tcgataacat
    13441 cgcccgccaa ctgaccacag gcagcatcga tatcatcacc acgagtttta cgcacaatag
    13501 tggtgaaacc gtagctcatc agcacttttg agaaacggtc gatacggctg ttcgaactgc
    13561 gtccatacgg cgcgccaggg aacgggttcc acgggatcag gttgatctta cacggcgtat
    13621 ctttcagtag ctccgccagt tggtgcgcgt gttcagtgcc gtcgttaacg tggtcaagca
    13681 tcacgtattc aatagtgact cggccctgat tggcgttgga tttctccaga taacggcgta
    13741 ccgcagcaag gaacgtttcg atattgtact ttttgttgat cggcacaatt tcgtcacgaa
    13801 tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac gtcgatcata tcgcccagtt
    13861 tatccagcgc cggaactaca ccggaagtgg aaagcgtcac gcgacgttta gacaggccaa
    13921 aaccgaaatc atcaagcatg atttccatcg ccggaacgac attgttcagg ttgagcagcg
    13981 gctcgcccat gcccatcatc actacgttag tgattggacg ctgaccggtg acttttgctg
    14041 cgccgacgat tttcgctgca cgccacacct ggccgataat ttccgatacc cgcaggttgc
    14101 ggttaaagcc ctgctgggcg gtggaacaga atttacactc cagcgcacac cccacctgcg
    14161 aagagacgca gagcgtggca cggtcgtctt ccgggatata caccgtttcg acgcgctgat
    14221 cgccaacggc aattgcccat ttaatggtgc cgtcagatga acgctgttct tcaaccactt
    14281 ccggtgcgcg gatctccgcc acctctttca gtttgccgcg caacactttg ttgatgtcgg
    14341 tcatctcatc aaagttgtcg cagcaatagt gatacatcca cttcatcacc tgatcggcgc
    14401 ggaagggttt ttcacctaaa tctttaaaaa actcccgcat ctgctgacgg ttgagatcca
    14461 gcaggttgat ttttccatct ttcgtggtga cgttttcagg tgtgactaat tgttcagaca
    14521 tatgctattc cggcctcgtt attacacgtt atggcccctg gagggttgaa aaaagaaacg
    14581 ccccggtgag cggctgctcg tccgggggcg ctgcattgta caaattctgg cgcacggatg
    14641 ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg cgcgggcaca cttcgccttc
    14701 gccgaagaaa taagcgattt cgcgagcggc agattcgacg gaatcagaac cgtgggtacc
    14761 attttcggtc aggctgtcag cgtaatcagc acgcagagta ccggccagtg cgtttgccgg
    14821 attggtcgcg cccagcagat cgcggtgacg ctgaacggcg ttttcaccct ccagcacgga
    14881 aaccacgatc gggccagagg tcatgaattc aaccagacca tcaaagaacg gttttccatc
    14941 gtgttcagca taaaagccac gagcctgttc aacggtcagg tgcagcattt tggtgccgac
    15001 aattttgaac cctgcagctt caaagcgcgc aaagatatta cctatgacgt tttttgctac
    15061 cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc atttttacct ctgtaaattg
    15121 ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat tataatgagc aacagggccg
    15181 ttgactattg atgaaggtaa cattttttta aaataaacct agtttaagca acaatcaggg
    15241 acaaaacaga ctattgcatg acaaatttca ctgtcgcgat ttgccccacc tcatccatca
    15301 ccagcaattg ataatcgccc ttatccatca aatgcagggt cacgttgcgc ccgcgttcag
    15361 ttaacggttc gccattcaga aaccaccagc gttcacctgc ccctccactt gattgcaacg
    15421 gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat cgcgccatcg cgaacacctg
    15481 tcagctgcag tgggagttgt gcatcgtgac cgtatggcgg gcaaattgtc gaggccggtg
    15541 gtaagcgcac agcgcggcgt tctgatgcgg gcaaccaggg ttccagcggt agcggccaga
    15601 catttatcat ttcttgtcgc gcctgagggc aatcagcggc aacacgtttg ccgttttcat
    15661 ccagccagat ggggaaacga atgccattaa tgccttcctg ctccggcagt aatagagttg
    15721 gtggctgact cccgtccagc agccaggttg ccaggcggcg gcggcagtta ccgtcacctt
    15781 ccggcaaaga ctgtccgccc ggccagcaga taacgccacg acttacagag tcaggacgtg
    15841 ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt attaacctga ttcaacaatg
    15901 gtacggcgct ggcaaagcca aactgaccaa caacgggcgt gccgtccggt ctgccagtcc
    15961 agatcccaat gacatagcga gcgttaaccc caatcgccca ggcatcacga tagccatagc
    16021 tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa ggcaccatcc ggcaagggtt
    16081 gcgcttcatc agccataatc cggcgaatga tccacgcagc ccccgacgac attaaaggcc
    16141 gttcaagcag cggatcgtca ggctgtaagc gcaatttacc tgccttgccg tggcgagcaa
    16201 acgcggtata cgctgccgcc atatcttcca gttttgcacc agcgccgccg agaatgagcg
    16261 aaagattcgg cgcagcgccg ttgggcagat acaacggcaa tccaacattg cgtaattttg
    16321 ccgcaaaccg tttcggtcca taggcttcca gcacctgcac agcaggtaaa ttcagcgagc
    16381 gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc gctatcaaag ttacctggtc
    16441 gataatcacc ggtgcgccgg ggaacgtctt gcagcagtga tgccgggtgg atcaagcctt
    16501 catccagcgc cagaccataa acaaacggtt tgagcaccga tcctggcgat cggatcgcat
    16561 tgaccatatc aacatgacca aagcgtgaat catcgttgag atcaacagaa cccacccagc
    16621 cgcgaacacg catatcggta tgatcaacca cgatcatcgc cagtgagctg cgcggtggta
    16681 atcgtccttt ccagttttgc gccagttctt ccagacgtcg ttgaagaccg gcatccagcg
    16741 tagtaacgat tttgtcgctt ttgcttttac cgagcatcat gcgcgaaaac agcggtgcca
    16801 gttgcggcat ttgtcggggt gccagccaga tgggttcttc ccttgattct tttacctgct
    16861 cacgggacca cacaccttgc gcagccatcc gttcgagcac tttattacgc gcggcttcgg
    16921 cacgttccgg ccagcgatcc gggcgaagac ggctgggagc ttgcggcaaa accgccagca
    16981 ttgccgcctc ggaatagctt aaattcgtag gcgattttcc gagataagcc caacttgccg
    17041 caccgatccc ctgcaacgta ccgccaaacg gggcacggtt aagatacaag gtcagaattt
    17101 cacgcttaga cagatgccac tccagttgca acgcgcgcca gagctggcga attttgccgc
    17161 caaatgtttt ggggtgagga tcaagcagac gagcaacctg catagtgagc gtgctgccac
    17221 cggaaataac ccgtcccgaa gtgagatctt gccatgctgc gcgcgccacc gagaatggat
    17281 tcacccccgg atgcttccag aaccagcgat cttcataatt gatcagcgct tcaaggtaac
    17341 gtggagaaac atcttcgatt gttaccggat aacgccagat gccttccgca tcggcaaagc
    17401 gccagagcgg cgtcccatcc tgcgccacaa ccactcgtgc gggattgact tcatgcagcg
    17461 gtagaggcca gagtttatct gctccccacg ctgcaagaaa aaggagaaag ggcgcagctg
    17521 ccagcgttat ccagcagccg cgtttggtta acaggcgagg catttacggt ctgacaatca
    17581 gcagatcttc agccgcgcca gtcgcccgcc attggggaac atacattgat tccaccatcg
    17641 gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc cagatacacc agcgttaccg
    17701 gttggtattc atcaacggca accgccgcca caaagcgatc atcacggaac tcaatgtgct
    17761 taatgctcgc ctgctgcatc tggttcagta agttctgcac ttcgccacca ctttgctcca
    17821 ggctggcgct accgttcgcc agattctggt tttccagttc cagacccgca ggcagcagat
    17881 ccacgactaa cgcatccggc acgctgttac tggcttttac ctgcaaccag accagcacca
    17941 gatcgccgct acgtaacgag tccagcgatt tactctgacc atcagtacca agaatatgac
    18001 gctcgatttg cagcacattg ttcgccggta aaggtgcgga ttgcggataa ccgctggcat
    18061 ccacacgcag ccataacggc tgatcgccac tgttggtcac ctgcaaggtg gcaagttgat
    18121 cgctattcag attgctgttt agcgttttct cgcctgtcag cggctcagct gagaaagagg
    18181 tttgcgcctg ccatttaccc ggtaaatcct gaattgtacg ggcagccagg aacaacgcgt
    18241 tactttcctg ggtcgatagc cagcgttcac caaatgcctg ctgcgaaagc gtgttcagca
    18301 aagtgttctg ctcatcgggt agcagtttat tttcttccag caaggagagc atcaacgcgt
    18361 tgtcgcgcag tggactaccg taatcaccca gccatatccg ctcatcacta ttacgcggcg
    18421 ttttcagcgc caacgcaatt gcttcttcac cacgcgtcgc atcacccatg gttttcagcg
    18481 caacgccaag ttgcagcagc ggtaaaccag aagcggcatc tgcacgatgc tcccagattt
    18541 cacgcagcgc acccagtggt gctttttgct gacgggccag caccagcgca gcgtaagact
    18601 gtacggcgaa tttactggct ttgagattat ccgcgtacgg gatcgacatc atgcccggat
    18661 cttgtaaata gcgcagcaga cgctcatttc cccggttaat gacgtctgtc ggcacgctgt
    18721 acccctgctc acctgcgcgg accaggaaat ccatcacgta agccgtcagc cagtactctt
    18781 cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc acgttgcatt tgcagcaaac
    18841 gggaaacgcc gatatcgacc gatgcacggc gtttctcatc actgtcgcct ttgatgccca
    18901 acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa caagccgctg gcggtttgct
    18961 caagacagcc gtacggatac gcttttaact ctttgatata acgtgcgatg ttcagcggtg
    19021 gtttgccgct caacaacaat tgcccttcca gtgtaacagg cgagaagttt tgcaatccat
    19081 ccgccggaat cgcccatgtc tcaccaggct gcaacgccgt accgtaatta accgtttgtg
    19141 ccgggaacgc cggacggacg ccgattttcc actgcttatg ctgatcggca acggtttcac
    19201 ccggtaacgc taacccgcta atggtggcct gaatttctcc atcgccataa cccggcaatg
    19261 ctcgcaccgg gataaacagc gtagtacgca cacctggcgc taattcaacg gctgcgggtg
    19321 aaacgctgac cagttcaagc aaaccactgg cggtcagggc aacgttcagt ttttgcggtt
    19381 tatcggtaag attagtgata tccagcgtca gacgcgaggt atcgccactc gccataaagc
    19441 gcggcatgtt cagttcagca atcaccggtg cggcaactat cactttgctt tcgttgctac
    19501 cgaagtcatc tgccgtccag gcttgcgcca tgacgcgtag ttcaccgtta aaatcgccaa
    19561 tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt taccggcagc gcctgctgcg
    19621 cgacaatatt gacgtgattg accggcggtt taccaccacg tttcagctca tcaccatcgc
    19681 caccaaaacg cagagctgcc agacgcccct gaccttcaat gacctgaccg taaatatcgt
    19741 aaatgtctgc accatagcgt ttctgaccaa agaacgcctg ccacggatcg ggggtaacgt
    19801 aatcagtaat attcagcaca ccgctatcaa cggcagacac cagcacattc acctgttttg
    19861 gcatctcacc atttttattg ctggctttaa ttttcacggt taatggctga ttggggcgca
    19921 ttttggtggg ggtttccagc gccagatcga gacgacggtt ttcatcgcca agcggcagat
    19981 gcaacacccc aactgcgcgt tttggcgtcg cagagcgaga tttatcgcca ggacgtacca
    20041 ccagcgtact taaatacaga tcatgacgat tccaggtttt atcgaccgga atcgtcagat
    20101 ccagcccttg agccggaaca tcaatctctt gccaccacag cggcccttca ctggactcga
    20161 ccatcgcata acctttaccc gccgttggcg cggcgatatg caacttaatg gtgtcgccag
    20221 ggcgataact ggctttatcc agtttcagcg tgacacggtc cggacgcact gcgccgctcc
    20281 cgtcgctgtt gtcctgccag ctatagccag cccagaaacg cacactactg accgcttcat
    20341 tcggcgcttt gacttccaga cgataagcgc cccactctac cgggaaactg accttgccgg
    20401 tttcgtccgc tttcagatcc agagtttgtt cattttcaat cagatctttt tgatcaaact
    20461 gtgattgcca gccttcatct tctgaccagt tccagtagta atcgcggcgt tcgcgaatca
    20521 ggcgcacctg caagcccgac acggcttttt tcacgccttg cgcatcgcta taaacgatgt
    20581 caaaagcggc gttactgcct tcatcaacaa tgggctgttt caccgtgctg tcagtgcgat
    20641 aatcgtaaac cgatttcgag gcgaactgcg gacggatccc cggcaatgca tcggcaggcc
    20701 agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc cgattccaac aaactaccct
    20761 ggaaaataac ctgtaatggg gaatgcgttt ccttccactg gctttctgta gaaacttcgc
    20821 cacgcccttt atcatccagc gtcaactgaa cttcatccag cgtgcgggaa agattttcgg
    20881 cagctatatc gccgaattcg aaaccaggta aggctgacac agcttcacgc agtgggcgca
    20941 ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc accatacagg tagtacccca
    21001 ccacggaaaa tctcacttca tctttcggcg ttagcggggt tttctcacca gtcagattca
    21061 gcgccatgcg ctctggcata aaatcttcga cgtggaaatc ccacatccgg tactggttat
    21121 cgcccgtgtt agcgcgaata tgccacatac cggttgccgc attgctatcg agtggccagg
    21181 taaagtggta gaggccattc tccggctgac taacgacgct cctgagtacc tgcccgtcgg
    21241 gtttaatcac gtctaacttg atgggttgat cgggcaatgc ttttccatct gcatcacgca
    21301 ataaaccatt gaggatgact gtttcaccag ggcgataaag atcgcgcggg ccaaacatga
    21361 aaaactgttt gctatagcct ggcgcgccag caatgttaaa ttctgctaag tccagcgccg
    21421 gaagttttaa atcgagtagc gttgtctgac cgtctttacg cgccagcagt aatgccgcgt
    21481 ttttatcatt ttccagctgc acatgcccct gagcatcact ggttgcctga gtcagagtct
    21541 gccctttctc atttaataaa gagacttgta ttccttgctg ggccgcgccg ttttccagac
    21601 tttgggtaaa gatatccaga cgattgtgat aacggtgagc tgaaacgccg atatcactta
    21661 acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc agcctgattc atcacagcca
    21721 gatacacgcc tgcctgctga agcggtttga tatcacccag cggcagcaat aatttttcac
    21781 gggtgttacg cgcaggattg agatcaaacc gtccggtata aaccagatcc gccatctgca
    21841 gcagtttttc agactgccag ttcgccagcg aattgcggta ttcccattgg ctaatgaatg
    21901 caggcagaga ttcgggcttc acacggaaga agttaacatc aacattgtta acgttgagcg
    21961 ccattaccgg cagcccttca ataactttac caggtaacaa cgaaccacgg ctggcaaaac
    22021 cgacgctggg ttggatatcg cgggtagtta tagttttttc gtaatcttta ctgaaggttg
    22081 cgttgttgag tgctttgacc tccttgccaa tagtgacgat caaatcacgt ttcggttcga
    22141 ggtggcgtaa acgcaactct ttaagattat ctgacagctc ccaggcacca tccactttgc
    22201 cgcttttctt atcgacgaca tggataacgc gtgagaaatc ctgatccggg tcgagaggaa
    22261 tggagaacgt cagcaccagc gtggctgcgc catcaagttg aacttcagag agatccagca
    22321 atgtcagcgc cttaccggca ctctgttggg ccagtttttg tcgctccggc gcggaaagtt
    22381 ttgctgaact cacgttttct gaagaggccg ctttagtaac ttcagaaggc gcatcttttt
    22441 tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc cagcgccagc attagcatgc
    22501 aggcggctac gcgtaacttt ttcatttttc atccctgcca caatggcccg ttagcaacgt
    22561 cgaataatta ttatgcgtga gaatttacgt tatgtaattc agtatcacgc ccatacaatc
    22621 cgaattttag tgagaattgt ggctcagaca gccaaatcag cctcttttgc ttgttgcatt
    22681 tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa tggagatgcc tatgtccacg
    22741 acatggtttg taggagccga ctggctcgcc gaacatattg atgacccgga aattcagatt
    22801 atcgatgccc gcatggcgtc gcctggacag gaagatcgta acgttgctca ggagtatctg
    22861 aatggacata ttcccggcgc agtgtttttt gatatcgaag cgctttctga tcacacttcc
    22921 ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg tggcgatgcg tgagttaggc
    22981 gttaaccagg ataagcacct gattgtctat gacgaaggta atcttttctc agccccacga
    23041 gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt cgattctggg gggtggactt
    23101 gcaggctggc agcgcgatga tctgctgtta gaagaaggtg cagtagagct gccggaagga
    23161 gagtttaacg ccacgtttaa tcctgaagcc gtggtgaaag tcaccgatgt gttattggca
    23221 agccatgaaa atacggcgca aattattgat gcccgcccgg ctgcacgttt taacgcagaa
    23281 gttgatgaac ctcgcccagg tttacgtcgc ggacatattc ccggcgcgtt taacgttccg
    23341 tggacagaac tggtacgtga aggcgaacta aaaacgaccg atgaactgga tgcgatattt
    23401 tttggtcgcg gcgtaagtta tgacaaacct attatcgtca gttgcggttc tggtgtaacg
    23461 gcagccgtgg ttttgttggc actcgcgacg ctggatgtgc caaacgtgaa actgtacgac
    23521 ggcgcatgga gtgaatgggg cgcacgggca gatttaccgg ttgagccatt gaaataagct
    23581 ttttacaggc aataaaaaac cgccgaattt ggcggttttt tattgctggt ctggttcgcg
    23641 gcctttccag ctggttgact tgtgttgctg gagcaacgca ggcaccccac agtgttgaaa
    23701 tactctcttt tatgcctggc tatgtcaaga taataaacag agtaattatt caaacatgag
    23761 agtattattc gtgacggccc ttttcgccgt ctcgcaaacg ggcgctggct ttaggaaagg
    23821 atgttccatg accgtaaatg cagacacccc acagtgcttg ctatcgcggc aatatcgcca
    23881 gtggtgctgt cgtgatgcgg tcttcgcatg gaccgcacaa tgaagatacg gtgcttttgt
    23941 atcgtactta ttgtttctgg tgcgctgtta accgaggtaa ataataaccg gagtctctcc
    24001 ggcaacaatt tactggtggt taacaacctt cagagcagc
//
LOCUS       DAOTRK010000069        23890 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.069, whole genome shotgun sequence.
ACCESSION   DAOTRK010000069 DAOTRK010000000
VERSION     DAOTRK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.069"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            286..1530
                     /gene="sstT"
                     /locus_tag="QIE41_002588"
     CDS             286..1530
                     /gene="sstT"
                     /locus_tag="QIE41_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDS3531465.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(1534..2085)
                     /locus_tag="QIE41_002589"
                     /pseudo
     CDS             complement(1534..2085)
                     /locus_tag="QIE41_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
     gene            complement(2168..3655)
                     /gene="uxaA"
                     /locus_tag="QIE41_002590"
     CDS             complement(2168..3655)
                     /gene="uxaA"
                     /locus_tag="QIE41_002590"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDS3531466.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(3670..5082)
                     /gene="uxaC"
                     /locus_tag="QIE41_002591"
     CDS             complement(3670..5082)
                     /gene="uxaC"
                     /locus_tag="QIE41_002591"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDS3531467.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5565..6863
                     /gene="exuT"
                     /locus_tag="QIE41_002592"
     CDS             5565..6863
                     /gene="exuT"
                     /locus_tag="QIE41_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDS3531468.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6993..7769
                     /gene="exuR"
                     /locus_tag="QIE41_002593"
     CDS             6993..7769
                     /gene="exuR"
                     /locus_tag="QIE41_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDS3531469.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            8114..8776
                     /locus_tag="QIE41_002594"
     CDS             8114..8776
                     /locus_tag="QIE41_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDS3531470.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            8780..9163
                     /gene="mzrA"
                     /locus_tag="QIE41_002595"
     CDS             8780..9163
                     /gene="mzrA"
                     /locus_tag="QIE41_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDS3531471.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            9310..9678
                     /locus_tag="QIE41_002596"
     CDS             9310..9678
                     /locus_tag="QIE41_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDS3531472.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLTEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            9716..10021
                     /locus_tag="QIE41_002597"
     CDS             9716..10021
                     /locus_tag="QIE41_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDS3531473.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            10024..10428
                     /locus_tag="QIE41_002598"
     CDS             10024..10428
                     /locus_tag="QIE41_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDS3531474.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            10418..10717
                     /locus_tag="QIE41_002599"
     CDS             10418..10717
                     /locus_tag="QIE41_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDS3531475.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            10903..11295
                     /locus_tag="QIE41_002600"
     CDS             10903..11295
                     /locus_tag="QIE41_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDS3531476.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            11365..12351
                     /gene="yqjG"
                     /locus_tag="QIE41_002601"
     CDS             11365..12351
                     /gene="yqjG"
                     /locus_tag="QIE41_002601"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDS3531477.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPSALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVVKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            12643..13008
                     /locus_tag="QIE41_002602"
     CDS             12643..13008
                     /locus_tag="QIE41_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDS3531478.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            13250..13606
                     /locus_tag="QIE41_002603"
     CDS             13250..13606
                     /locus_tag="QIE41_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDS3531479.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPVIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(13657..14553)
                     /gene="yhaJ"
                     /locus_tag="QIE41_002604"
     CDS             complement(13657..14553)
                     /gene="yhaJ"
                     /locus_tag="QIE41_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDS3531480.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            14658..15359
                     /locus_tag="QIE41_002605"
     CDS             14658..15359
                     /locus_tag="QIE41_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDS3531481.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            15382..15546
                     /locus_tag="QIE41_002606"
     CDS             15382..15546
                     /locus_tag="QIE41_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531482.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTANNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(15606..16916)
                     /locus_tag="QIE41_002607"
     CDS             complement(15606..16916)
                     /locus_tag="QIE41_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDS3531483.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(16944..18275)
                     /locus_tag="QIE41_002608"
     CDS             complement(16944..18275)
                     /locus_tag="QIE41_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDS3531484.1"
                     /translation="MEIVSNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(18551..19915)
                     /gene="tdcG"
                     /locus_tag="QIE41_002609"
     CDS             complement(18551..19915)
                     /gene="tdcG"
                     /locus_tag="QIE41_002609"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDS3531485.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(19987..20376)
                     /locus_tag="QIE41_002610"
     CDS             complement(19987..20376)
                     /locus_tag="QIE41_002610"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDS3531486.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(20390..22684)
                     /gene="tdcE"
                     /locus_tag="QIE41_002611"
     CDS             complement(20390..22684)
                     /gene="tdcE"
                     /locus_tag="QIE41_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDS3531487.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(22718..>23890)
                     /gene="tdcD"
                     /locus_tag="QIE41_002612"
     CDS             complement(22718..>23890)
                     /gene="tdcD"
                     /locus_tag="QIE41_002612"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDS3531488.1"
                     /translation="GSSSIKFSVLDASDCEVLMSGIADGINSENAFLSVNGGEPAPLA
                     HHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITDEVIDNIRRVSPL
                     APLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLPWKYYEELGVRRY
                     GFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVCNGQSVDTSMGMTPLEG
                     LMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLSSDLRVLEKAWHE
                     GHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLIRRLVMEHLAVLG
                     VEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHLGKVNASAEFA"
BASE COUNT         5734 a         6313 c         5941 g         5902 t
ORIGIN      
        1 atatggcata gcacattaaa ggagcgcaac cgttctcata ttccaaatag ttttgcccat
       61 atgttatcta atcagcctta gtagccagtc cttccctctg tttatgtcaa ataaatgtaa
      121 ccattaagtt gcaaaatatg acctctcttt aaaatccagc atttttcgct tcccgaagct
      181 gtaactttcc ttatactcga ccttgcaaac actttgttac atcctgaaag atgcgtcgac
      241 agaacgcacc agggatgtgc gacaacacaa tgaaaggatc gaaaaatgac tacgcaacgt
      301 tcaccggggc tattccggcg tctggctcat ggcagcctgg taaaacaaat cctggtcggc
      361 cttgttctgg ggattcttct ggcatggatc tcaaaacccg cggcagaagc tgttggtctg
      421 ttaggcactt tgttcgtcgg cgcactgaaa gctgtagcgc caattctggt gctgatgctg
      481 gtgatggcat ctattgctaa ccaccagcac gggcagaaaa ccaatatccg ccctattttg
      541 ttcctctatc tgctgggcac cttctctgct gctctggccg cagtaatctt cagttttgcc
      601 ttcccttcta ccctgcactt gtccagtagc gcgggtgata tttcgccgcc gtctggtatt
      661 gtggaagtga tgcgcgggct ggtaatgagc atggtttcca accccatcga tgcgctgctg
      721 aaaggtaact acatcgggat cctggtgtgg gcaattggcc tcggtttcgc actgcgtcac
      781 ggtaacgaga ccaccaaaaa cctggtcaac gatatgtcga atgccgtcac ctttatggtg
      841 aaactggtca ttcgcttcgc accgatcggt atttttggtc tggtttcttc taccctggca
      901 accaccggtt tctccacact gtggggctac gcgcaactgc tggtcgtgct ggttggctgt
      961 atgttactgg tggcgctggt ggttaaccca ttgctggtgt ggtggaaaat tcgtcgtaac
     1021 ccgttcccgc tggtgctgct gtgcctgcgc gaaagcggcg tgtacgcctt cttcacccgc
     1081 agctctgcgg cgaacattcc ggtgaatatg gcgctgtgtg aaaagctgaa tctggatcgc
     1141 gatacctatt ccgtttctat tccgctgggt gccaccatca atatggcggg cgcagcaatc
     1201 accattaccg tgttgacgct ggctgcggtt aatacgctgg gtattccggt cgatctgccc
     1261 acagcgctgc tgttgagcgt agtggcttct ctgtgtgcct gtggcgcatc cggcgtggcg
     1321 gggggttctc tgctgctgat cccactggcc tgtaatatgt tcggtatttc gaacgatatc
     1381 gccatgcagg tggttgccgt cggctttatc atcggcgtat tgcaggactc ttgcgaaacc
     1441 gcgctgaact cttcaactga cgtgctgttc actgcggcag cttgccaggc ggaagacgat
     1501 cgtctggcaa atagcgccct acgtaattaa tgtttaaccc ctttcgtcca cggcggaagg
     1561 ggttttctcg actttaaacg gatcaattcc ccttttctgc atccgccaga aacgaatgat
     1621 attcaggcca ttcatgagca gaaaactccc ctcaatcatc gtgccgccta tctaccccgc
     1681 ccagaagttg tgaatcaccc agcaacacgt tgaaaaccac attacgcagc gcatggtcag
     1741 ccctttacag cggaacagcg cccaggtact gacaatcgtg ccgattaccg gcaatagttc
     1801 gacaggatga tggaacttcg cgaggccaat tccgccagtc agcacaataa aaatcgccat
     1861 tacccataag ctgcgcgtgc gtaaagtaat cagcgtacga atggcattaa ggatggcact
     1921 ggcaccggcg ggataggtgc ccagaagaaa aaaatgtacg ccaataacgg cgctatagac
     1981 cgaaagctgc tttttaaagc ggcgttcgtc acggttgaaa aatgttgtga taccaatcag
     2041 aaaggcgatg acacccacgc cctgggccag ccaatacgcg gtcatgataa atccttagca
     2101 ggtctggaaa agcaaacggc gcttcacatt atgaaacgcc gttttttatt aacaactcat
     2161 ttcgacttta tagcgttacg ccgcttttga agatcgccag ttcgcggaag tcgttacgct
     2221 cgttacaggt ttgcttaccg ttggcaaact caacgatggt atcgatgaat tcttccagca
     2281 actgcggcat cgctttaccg tggatcagct gacccgcgtc aaagtcgatc cagtgttttt
     2341 tcttcgccgc cagttcgctg ttggtggcga ttttcaccgt cggtacaaag ccgccgtatg
     2401 gcgtgccgcg accagtactg aacagcacca tatggcagcc cgcacccgcc agggcgctgg
     2461 tcgctacggc atcgttaccc ggcgcactta acaagttcag ccccggtgtt ttcagacgct
     2521 caccgtaacg cagcacgtca accacgacgc tggaacccgc tttctgggta cagccaagtg
     2581 atttgtcttc cagcgtggtg ataccgcccg ctttgttccc cggcgatggg ttttcataga
     2641 tcggctggtc atgggcaata aagtactgct tgaagtcatt gaccatggtg accagttttt
     2701 caaacgttgc ttcgtcgcgg caatggtcca tcagcaactg ctctgcgcca aacatctccg
     2761 gcacttcggt cagtacggta gtaccgccgt tagcaatcac gtagtcagag aaacgcccca
     2821 gcatcgggtt agcagtaata ccagaaagac cgtcagaacc accgcactcc agaccaaact
     2881 tcagttcgct gagtttgcct ggctcgcgtt tatcgttgcg catcacgtta tacagctgat
     2941 gcaaatgctc gatcccggcc tcgatctcat catcctgctg ttggcagatc atgaaatgaa
     3001 cgcgttcagg atcgatatcg cccagcgttt cacggaatgc ggcaacctgg ttgttttcac
     3061 agcccagacc aatcaccagc actgcgcctg cgttcgggtg gcgcaccatg ttttgcagca
     3121 tggtgcgggt attaatgtga tcgtcgccca gctgtgagca gccgtaggtg tggctgaaga
     3181 ggaacacgcc gtcggtacct tcggcgttgt tggtctcttt caggaaacga ttttggatct
     3241 gccgcgcgat gccgttgaca cagcccacgg ttggcaggat ccacagctca ttgcgcaccc
     3301 cgacatcgcc gttagcgcga cgatagatct gcacttcacg atctgccgct tgcgcaggca
     3361 gatcctgaaa atcaggttga tagcgatact gatccagatc gctcagattc gtgcgcgtat
     3421 tgtgggcgtg aacgtgttcc cccgccgcaa tatccgccaa tgcataacca atcggcaggc
     3481 catatttgat gacgttggcc ccttttgcga tatccgttaa cgcaaattta tgtccacgag
     3541 caacatcctg gcgcagcgta acagtctggt tgtcgacact gacttctgtg ccttccgcca
     3601 aatctgctaa agcgaccgcg acgttatcca gcgcatggat cttgatgtat tgcatatcaa
     3661 ccccagacct tagttcagtt caatggcgaa gtagtcacgc gcattgttaa agcaaatatt
     3721 tttcaccatc tcgcccagca ggttgatatc cgccggcgct tcgcccgctt ccacccagcg
     3781 accgatcatc tggcacagaa tgcggcggaa gtattcgtga cgggtgtatg acaggaagct
     3841 acggctgtca gtcagcatac cgacaaagcg gctcaacaga ccgagctgcg ccagttgggt
     3901 catctgacgt tccataccgt ctttctgatc gttaaaccac cagccggaac cgaactgcat
     3961 tttgcccggc ataccttcgc cctggaagtt accgatcatg gtgcccagca cttcgttatc
     4021 gcgcgggttc aggcagtaga gaatggtttt cggtagcagg ttttcttcgt tctgcttgct
     4081 cagcagctta gacagctctt ccgccatcgg acggtcgttg atggagtcaa agccgacatc
     4141 cggccccagc agtttaaact gacgcaggtt gttattacgc agtgcgccaa tgtggtactg
     4201 ctgtacccag ccgcgacgtg catattcggc accaaggaac accagcaccg cagttttgaa
     4261 ctgtgccact tcgtgctcgc tcagggtttc gccagccaga cgacgcgcca gaatgctgtc
     4321 cagttccgct tcgttcgctt cagcaaacat caccacatcc agcgcgtggt cagacacttt
     4381 acagccgtga gcggcgaagt gatccagacg tttggtcagg gcagtttgca gatcagcaaa
     4441 gcggcggatg tcggtatcgg aaacttcgcc cagcttcgcc atgtagtcgt taaaggtcgc
     4501 ctgttcgatg ttgaaggctt tatcaggacg ccagctcggc agcactttaa tggtgaaaga
     4561 gccgtctttg gcgatctctg cgtgatgctc cagagaatcg atcgggtcat cggtggtgcc
     4621 gaccattttc acgttcatct gctgcatgat gccgcgcgcg gagaagttat cctgcgccag
     4681 cagttcatta cattcgttcc agatttcatc ggcagttgac ggagaaagca atttgccagt
     4741 gataccaaac ggacgacgca gttcgaggtg cgtccagtgg tataacgggt tgccgatagt
     4801 gtgcggaaca gtcgccgccc aggcgtcaaa tttttcacgg tcagacgcat caccagtaca
     4861 cagacgctca gccacaccgt tggtacgcat agcgcgccat ttgtagtgat cgcccttcaa
     4921 ccagatgtca tacaggtttt taaaacgata gtcttccgca atctgctgcg gcggcaaatg
     4981 gcaatggtaa tcgaaaatcg gctggtcttt tgcgtagtcg tgatacagac ggcgggcaaa
     5041 ttcggtatct aacaggaaat cttcagtcat aaacggagtc attttcgtct tcctctcaac
     5101 gagtgagcta gcttgcttat ggtgcgatgc tgacaaagtt atcacaccaa tttccagagt
     5161 ccgaagatat tttcgtgaga tagatcaata aacgtagtta aaaaaattac tctcaaagtg
     5221 gtaaatctcg ctgcaggccg cgccagtact ggcctcgctg tcgtcaggta atgtccctac
     5281 aaatattccc acatttgtga tggctctcac cttttaaagt tgtatgacaa gttatctttc
     5341 tgccgtcgca aatcataagt cgacggaatg caaattgccg attcattcat ttgttagatg
     5401 aatcgggtta accggtacgg aagccgaatt agcacgaaac tttcatggca acgttcgggg
     5461 cgtgccggtt ttttttcggt tacccggtcg taactaacat cttcagcctc tggcgggatg
     5521 atggccgcgc ttcctgcgga tataacaaaa cgatgaggtt ttacatgcgt aaaattaaag
     5581 ggttacgttg gtatatgatc gcactggtga cgctcggcac cgtgcttggt tacctgacgc
     5641 gtaacactgt ggcggcagct gcgccaactc tgatggaaga gttaaacatc tccacccaac
     5701 agtattccta tatcatcgca gcctattctg ctgcttatac ggtcatgcaa ccggtagcag
     5761 gttatgtact ggatgtgctg ggtacgaaaa tcggttatgc aatgtttgct gtgctgtggg
     5821 ccgtgttctg tggtgcaacc gcgctggcag gtagctgggg cggcctggct gttgctcgtg
     5881 gtgcggtcgg tgccgcggaa gcagcgatga tcccggcggg tctgaaagcc agctccgaat
     5941 ggttcccggc gaaagagcgt tccatcgcgg taggttactt taacgtaggt tcttctattg
     6001 gtgcgatgat tgcaccgccg ctggtggtat gggcaatcgt aatgcacagc tggcagatgg
     6061 catttatcat ctccggtgcg ttgagcttta tctgggcaat ggcatggctg attttctata
     6121 aacatccgcg cgaccagaag catctgaccg atgaagaacg cgactacatt attaatggtc
     6181 aggaagccca gcaccaggtt gacacagcga agaaaatgtc cgttggtcag atcctgcgta
     6241 accgtcagtt ctggggtatc gcgctgccgc gcttcctggc agaaccggca tggggtactt
     6301 ttaacgcgtg gatcccgctg ttcatgttta aagtttacgg ctttaacctg aaagagatcg
     6361 cgatgtttgc ctggatgccg atgctgtttg ctgacctcgg ttgtatcctc ggtggttacc
     6421 tgccgccgct gttccagcgt tggtttggtg tgaacctgat cgtttcccgt aagatggtcg
     6481 taacgctggg tgctgtgctg atgattggcc cgggtatgat cggtctgttc accaacccgt
     6541 atgtcgcgat tatgctgctg tgtatcggtg gttttgctca ccaggcactg tctggcgcgc
     6601 tgattacgct ctcttctgac gtgtttggtc gtaacgaagt ggcaacggca aacggtttaa
     6661 ccgggatgtc cgcatggctg gcaagtacgc tgtttgcgct ggttgtcggt gcactggctg
     6721 acaccatcgg cttcagcccg ctgttcgcag ttctggcagt attcgacctg ctgggtgcac
     6781 tggttatctg gacggtgttg cagaacaaac cggcgattga ggtggcgcag gaaacacata
     6841 acgatcctgc accgcaacat taaggcttcg tgtaagtttt agtttccctt caaagccgcc
     6901 ttctcaggcg gctttttcat cactgtgagt agagctaaac tcgcaaaagt ggtataacaa
     6961 atatagtctg ccgtatcatg cctggagcgc atatggaaat cactgaacca cgccgtttgt
     7021 atcaacaact tgccgctgat ctgaaagagc gcatcgaaca gggcgtctat ctggtgggtg
     7081 ataaactgcc tgccgaacgc tttattgccg atgaaaaaaa cgtcagccgc accgtggttc
     7141 gtgaagccat catcatgctg gaagttgaag gctatgtcga agtgcgtaaa ggttcaggta
     7201 ttcatgtggt ttccaaccag ccgcgccatc agcaggcggc tgacaataat atggaattcg
     7261 ccaattacgg tccgtttgag ttgcttcagg ctcgccagct catcgaaagt aatattgccg
     7321 aattcgcggc aactcaggta acgaaacagg acatcatgaa actgatggcc atccaggagc
     7381 aggcgcgcgg cgaacaatgc ttccgtgatt ccgagtggga tttgcagttc cacattcagg
     7441 tagccctggc gacgcagaac tccgccctgg cggctatcgt ggaaaaaatg tggacccagc
     7501 gtagtcataa cccgtactgg aaaaaactgc acgaacacat tgatgcccgt accgtcgata
     7561 actggtgtga tgaccacgat caaatcctca aggcgttaat tcgcaaagat cctcatgctg
     7621 ctaagctggc aatgtggcag catctggaaa acaccaaaat catgttattt aacgaaacca
     7681 gcgacgattt cgagttcaat gccgaccgct atctgttcgc agaaaacccg gtagtgcatc
     7741 tcgatacggc caccagcggc agcaaatgaa atttccatcc gacaggcgct gcaatgcgcc
     7801 tgtttgcttc gtcggtaagc gaaaagtata aagtgtcagc ctgtgtaaat cctctcgcca
     7861 ccctcccctg caatcagcaa aatcagactc tacggacatg taattttgat aacgaacaac
     7921 gttgaccttt gttacaatta gattcaattt gaatttatgc ttttgaatgc tttcttatct
     7981 cacgatttaa cagggaatag ttcaggctgt gttgatgtat caaacccgca aaacatacca
     8041 aaacagcaat aacattgcgg tagtgcatct tttaaaacca gcgtggcgtt aaccgattca
     8101 ccaggaataa tgaatggaac ttttgaccca attgctgcaa gccctgtggg cgcaggattt
     8161 tgaaaccctg gccaatccgt cgatgattgg catgttgtat tttgtcttgt ttgtaatttt
     8221 gttccttgaa aacggcttgc ttccggcggc ctttttaccg ggcgacagtt tactggtgtt
     8281 ggtcggcgta ttgattgcga aaggcgcgat gggctatccg caaacgattc tgctgctgac
     8341 cgttgccgcc agcctcggtt gctgggtcag ctatattcag gggcgatggt tgggcaatac
     8401 ccgcactgta caaaactggc tatctcattt acccgcgcat tatcatcaac gcgcacacca
     8461 tctttttcat aaacacggtt tatcggcgct gttaattggt cgctttattg catttgtcag
     8521 aacactgctg ccgacgattg ccggattatc agggctgaat aacgcgcgct ttcagttctt
     8581 caactggatg agcggtctgc tgtgggtatt gatcctgaca actctgggtt acatgctcgg
     8641 caaaacgccg gtatttttaa agtacgagga ccagctgatg tcatgcctga tgctgctccc
     8701 ggtggtgctg ctggtgtttg gcctggcagg ttctctggtc gtgttatgga aaaagaaata
     8761 tggaaatcgg gggtaaggga tgcaaatacc tcgcatgtcg cttcgccagc tagcctggtc
     8821 cggcgctgtt ttacttctgg tcggcacact gttgctggcc tggtccgcgg ttcgccagca
     8881 agagtctacg ctggcgattc gtgccgttca tcaagggaca acgatgccag acggtttttc
     8941 aatctggcat caccttgacg ctcacggcat tcctttcaaa agtatcaccc ccaaaaatga
     9001 cactctgtta attacctttg actccagcga ccagagtgcc gccgcaaaag cggtcctcga
     9061 cagaacattg ccccatggct acatcatcgc gcagcaggac aataatagtc aggctatgca
     9121 gtggctgacc cggttgcggg ataactctca tcgcttcgga taacttcctg ttatccgaaa
     9181 catatcactg actttggtga tttcaccgta actgtctatg attaatgagg cggtggttaa
     9241 cactaccgct tcgttgtctg gacaatcgtt cctttgtaat aggtccgaat cacaatggaa
     9301 ggttcaagaa tgaaataccg catcgcttta gctgtttctc tctttgctct tagtgccggt
     9361 agttatgcca ctaccctgtg tcaggaaaag gagcaaaata tccttaagga gatcagctat
     9421 gccgaaaaac accaaaacca gaatcgtatt gacggtctga ataaagccct gagtgaagtc
     9481 cgggccaact gttcagatag ccagctgcgt gccgatcatc agaagaaaat cgcaaagcag
     9541 aaagatgagg tggcggaacg ccagcaagat ttaaccgagg cgaagcaaaa aggcgatgcc
     9601 gataagattg ccaaacgcga acggaaactg gcagaagcgc aggaagagct gaaaaagctg
     9661 gaagcgcgcg actactaact cacaatagtc actacttact cacctggaga aaactatgtc
     9721 gaaagaacac actacggaac atctgcgtgc tgagttgaaa tccctttccg atacgctgga
     9781 agaggtactt agctcatctg gcgagaagtc gaaagaagag ttgagtaaga ttcgtagcaa
     9841 agcggagcag gcactgaaac agagccgtta tcgcctgggt gaaaccggtg atgccatcgc
     9901 caaacaaacc cgtgtcgcgg cggcgcgtgc cgatgagtat gtgcgtgaaa acccgtggac
     9961 gggcgtgggc attggcgctg caatcggtgt agtgctcggc gttctgctgt cgcgtcgtta
    10021 attatggcgg acactcatca cgcgcaaggg cccggtaaaa gcgttctggg catcgggcag
    10081 cgaattgttt ccatcatggt tgaaatggtg gaaacacgtc tgcggctggc ggtggtggag
    10141 ctggaagagg aaaaagcgaa tctctttcaa cttttactga tgctgggcct gacgatgctt
    10201 ttcgctgcat ttggtcttat gagcctgatg gtgctgatta tttgggcggt tgatccgcaa
    10261 tatcgcctga atgcgatgat tgccaccacc gtggtgttgc tgctactggc actgattggc
    10321 ggtatctgga cgttacgtaa atcgcgtaaa tccaccctgc tgcgccatac acgccatgag
    10381 ctggcaaacg atcggcagct gctcgaggag gagtcccgtg agcagtaaag tcgaacgtga
    10441 acgacgtaag gcgcaactgc tgagccagat ccagcaacaa cggctggatc tttccgccag
    10501 tcgtcgtgaa tggctggagg caacaggcgc ttacgatcgt cgctggaata tgctgctaag
    10561 tctgcgctcc tgggcgctgg taggcagtag cgtgatggcg atctggacga ttcgccatcc
    10621 taatatgctg gtccgctggg ccagacgcgg ttttggcgta tggagcgcct ggcgtctggt
    10681 gaaaacgacc ctcaagcagc aacaacttcg cggttaactt ccctctggcc ggagccattc
    10741 cggccttatc cctcaaattt tttgaagatt tttgacagtt ttccttgcta acattcatca
    10801 ttcaccacgt ttatgattct ctccatcgac agcaacgacg ctaataccgc gcccttgcac
    10861 aaaaaaacaa tcagcagcct gagtggcgca gtggagagta tgatgaaaaa attagaagat
    10921 gttggtgtac tggtagcgcg cattttaatg ccgattctgt ttattaccgc aggctgggga
    10981 aaaattactg gctacgcggg tacccaacaa tatatggaag cgatgggtgt cccaggcttt
    11041 atgctgccgc tggtgattct gcttgagttt ggtggtggtc tagcaatcct gttcggtttc
    11101 ctgactcgca ccacagccct gtttactgcg ggctttacgc tgctgacggc atttttattt
    11161 cacagcaact ttgctgaagg cgtcaactcg ctgatgttca tgaaaaacct gacaatttct
    11221 ggcggattcc tgctgctggc aattaccggt ccgggcgcgt atagcatcga ccgcctgctg
    11281 aataaaaagt ggtaagcacg agctatactt aacgataaaa aacgaggagg aagctcctcg
    11341 tttttgctat tggaggagag aaatatgggt caactgattg acggcgtctg gcatgacacc
    11401 tggtacgata ccaaatctac cggcggtaaa tttcaacgtt cagcttccgc atttcgtaac
    11461 tggctcactg ccgatggcgc gccaggcccc actggcacag gcggttttat cgcagagaaa
    11521 gatcgttatc atctctatgt ttcactcgcc tgcccgtggg cgcaccgcac gctgatcatg
    11581 cgtaaactca aaggactgga accgtttatt tccgtttccg tagtgaaccc gctgatgctg
    11641 gaaaacggct ggacatttga tgacagtttt ccgggtgcaa ccggagatac gctctatcaa
    11701 catgaatttc tgtatcagct ttatctccac gccgatccac actacagcgg acgagttact
    11761 gttcccgtgc tgtgggacaa aaagaaccac accatcgtca gcaacgaatc agcagaaatc
    11821 atccgcatgt tcaataccgc gtttgatgcg ctgggcgcga aagcgggtga ttactaccca
    11881 tcagccctgc aaacgaaaat tgacgaactt aacggctgga tttatgacac cgttaacaat
    11941 ggcgtgtata aagccggttt tgccaccagc cagcaagcct acgacgaggc ggtagtgaaa
    12001 gtgtttgaat cgctggcgcg actggagcag attttaggcc agcatcgtta cctgaccggc
    12061 aaccagctaa ccgaagccga tattcgcctg tggaccacgc tggtgcgttt tgatccggtg
    12121 tatgtcaccc acttcaagtg tgataagcac cgcatcagcg attacctgaa cctgtatggt
    12181 ttcctgcgag atatctacca gatgccgggg attgccgaaa ccgtcaattt cgatcacatc
    12241 cgtaatcatt acttccgcag tcataagacc atcaacccta cggggattat ttcaattggt
    12301 ccgtggcagg atctcgatga accgcatgga cgagatgttc gcttcggtta aaaaaaggca
    12361 ccttacgggg tgcctctgat tattagctaa gtgcgtgtta tttatgtcgg atgcggcgtg
    12421 aacgtcttct ccaacctaca aaacgtttat attcaatatg ttacaatgcc gcgtaggcca
    12481 gataagcgta gcgcatctgg cgattttgcg cttgtcatca gtcttagcgc gccttcacgg
    12541 cactttatta attccttgaa cgaatattta ctgccatttc attcacgtct attcttaatt
    12601 tgctgcttta aaaacaagtg attgagcaaa attgaggcaa aaatggactg gtatctgaaa
    12661 gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta ctggatgttt
    12721 attctggtca acatcatctt tacgttcgtg ctggggctac tggataaaat gttaggctgg
    12781 caacgtgccg gtggcgaagg catcctgacg acaatctacg gtattctggt gtttttacca
    12841 tggtgggcag ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg gtgggcactg
    12901 ctattcttaa tcccgtttat cggctggctt attatcatcg tctttaactg ccaggcgggt
    12961 acgccaggcg aaaaccgctt tggacctgat ccaaaactgg aaccttaaaa caaatctact
    13021 cgccggatgt ctgactcatc cggcttttat catcatgttg atgaatgaat aatttactaa
    13081 tgtttattta aaatatttca atgtcgttat ataaattaca tataaaaaat aaccatgtat
    13141 ttatcttaaa tatcaaaacc tttataacat ccgaaaaaga aagatacgaa tagcatttac
    13201 acaatacccg gaatagactt tcgtcacttt taattaaagg gatgttttta tgcagtggta
    13261 tttagccgta ttaaaaaatt atgttggttt ctctggtcgc gcacgtcgta aagagtactg
    13321 gatgtttact ctgattaacg ccatcgtcgg cgctattatc aatgtcattc aattgatttt
    13381 aggtttagag tttccattcc tttctctcat ttacctggca gcaacaatca ttccggtcat
    13441 tgctctgtgc gttcgtcgtc tgcacgatac tgaccgttca ggtgcgtggg cgcttctcta
    13501 tctggtaccg gtcattggct ggttagttct gtttgtattt gcctgtttag aaggcaattc
    13561 tggcagcaat cgttacggaa acgatccgaa gtttggttca aattaatctt agaattgggg
    13621 cgatatttcg ccccttttta ttaacaataa caaatattat tttccgctaa aaagtttggg
    13681 aatttcccgc aaacaccagg atttcgcttc ccccatactg tcacgacgcc aggccataat
    13741 aatatcgatc tcgctggtcg attctgggct gacgacacgc aaccgccctt ccgcaatatc
    13801 tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta acgcctgacg
    13861 tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcagct gtacggtcaa
    13921 caccgggcgc tcacgagcgg tatccgctac cgcaattcca cgatatttca cgcgcgtcac
    13981 ttcagataac ggttctggct cctgatgaat cgggtgatca ggcgcggcaa catagacgtt
    14041 cattaaggta tagagtttgc gcgagttgat ctccgacgag gaacgaaaat gcatatccgg
    14101 cgcgataacg atatccgccc gcccctgctc cagccgttcc catgcccccg ccagcacttc
    14161 ggtgatgatt gccagttggg tattcgcttt tgccgccagt ttgtcaatta acgggaaaaa
    14221 ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc
    14281 gagagcttcc gcatcggtag tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc
    14341 cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt caaacagcac
    14401 cacatccagc tcttcttcca gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc
    14461 cagctcatcc gccgccgccg caaaactgcc ccggcgatcg atcgcatcca taacccgtag
    14521 tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga aatttgaaca
    14581 taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata aaaggaagta
    14641 aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga caagcagact
    14701 acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac ccgaaattgt
    14761 taggctatgc ctccctgcgc gtgcttaacc aggaagtgct ggccccaggt gccgcctttc
    14821 agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga gaagcagagt
    14881 atcgcgacag cgaaggcaat catgttcagg ccagcgctgg tgaggtgttg ctgctctcta
    14941 cccagccggg tgtcagctat agcgaacaca atctcagcaa agacaaaccg ttaacgcgaa
    15001 tgcagctttg gctggatgcc tgcccgcagc gagagaatcc actgattcaa aagctggcgc
    15061 ttaatatgga caagcagcaa ttaatcgcct cgccagaggg tgcgatggga agcctgcaat
    15121 tgcgccagca agtgtggctg catcatattg tgctcgacaa aggcgaaagt gcgaatttcc
    15181 agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat gcacttacgc
    15241 atcatgaaga gaaagcggcg ctgacctgcg gtgatggggc gtttattcgt gacgaggcta
    15301 acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg cctgtctagt
    15361 tgttttaaca gggagatgat gatgagtaaa aaatcggcca aaaagcgtca gccagtgaag
    15421 cccgtggtgg cgaaagaacc tgctcgcacc gccaataatt ttggctatga agagatgttg
    15481 agcgagctgg aagccatcgt cgcggatgct gaaacgcgtt tagccgagga tgaagctact
    15541 gcgtaaagac aggtgccgga tgcggcgtaa acgccttatc cggcacgata cccaaaacct
    15601 ggcgattatc tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc
    15661 gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg cgccacaatc
    15721 ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc
    15781 gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg ctccatcgca aatcatgccg
    15841 ctgacatcgc cgatcatact gctgatcgcc atcgaaatgg tttcataacg cccatccacc
    15901 agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca aagcgcagac
    15961 aaacgcggta actggttatg gatgtaaatc gcgctcaaat gcgaaagcat cagcgcacgc
    16021 gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacagg cattgttgcg
    16081 gtgatcccct ggttacccga gccggagtta ctcattgccg gaagcgtagc gccgcccata
    16141 cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc
    16201 aaaccgcgcg cgcactgttt ttccagcgtc gcaccaatat gcagccccca gttaccgctc
    16261 aaaccttcct gtgataacgc gcaatttaac ttcgcagaat cgagaataaa gcggatcgcc
    16321 gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgtcct ggaaagcacc
    16381 gtcagcggcg actcttgctc gccctctgtc acacacgcct gctgagtaaa caccacacca
    16441 ttgtgcgtct caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac
    16501 ttctcaccgt tccagacttt ggcgcgtgag aagaggattt catcgcaagg ttcctggatc
    16561 ttaactgaga ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt
    16621 gcgtctttca gcacttccag cccggcgtta gcatttccac ctaacgcccc aagcgccgcc
    16681 gcaatcggca gccccaccat tcctgtaccg ggaacggtga cacccagacc gttcttcatc
    16741 agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc agccgcagca
    16801 accgccgccg ccagcgccag tgaaatcggt tcagtacacc ccagcgccgg ttttacctcc
    16861 tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta
    16921 aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac acaaagcaac
    16981 aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact
    17041 ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata
    17101 gaggtgaagc tcaacaccgg ggcgttcagt acgatggcgc tccaggccag caaaatggcg
    17161 aaaatcatga tgccgcgctg aacgagattt tcgttaatct tctcggcagg tatcttgcga
    17221 cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag
    17281 aatgcagtca ttacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg
    17341 ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct
    17401 tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg
    17461 acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgctacttca
    17521 atcgattttt cccgcgagcg ataagaaatc accatcgggc ttaacgtctg gataaacaga
    17581 atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc
    17641 ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc cagcgccgcg
    17701 accaccagca gcttggtcag caccatcccc gtcgaaattt tgaataacaa tttctcgccg
    17761 cgtgaggaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac
    17821 agcaaccctt ccgtcacgcc gaaggtatgc aggtaggaag cactatcgtt ggtgatggcg
    17881 gtggaataga cgaacatcca aatcaccagc attacgaaat agagtgcacc taacaggatg
    17941 ccccagtttt tacctaaata accgctaatg acgctcgggt aatctttaca ttctggtgat
    18001 tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc
    18061 gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc
    18121 cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat
    18181 ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg
    18241 tctgcaatga cgcctttatt cgatacaatt tccataattt ctcgctcaat attttgtagg
    18301 gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga
    18361 gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt
    18421 tgaaataacg gagatggata agaattttct actaaattaa tcacaagaga atattcattc
    18481 tctatttgcg gcagatcaca aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct
    18541 gacagcaatg tcagccgcag accactttaa tggccagtcc tccgcgtgat gtttcgcggt
    18601 atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa
    18661 cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat
    18721 taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca
    18781 gcccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccgc
    18841 ccaggagttc agttaaccct gccgccgcca ttgaacaggc cacgccaatt tccccctgac
    18901 agccgacttc cgcgccggag atggaggcgt tcattttata cagcgcgcca atagcccccg
    18961 cggccagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat
    19021 aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc
    19081 cgccagctgc gttttcttca ctaaccgcca gcgcgtacat attgatccag tcgatgacat
    19141 tcatcggatc gttagagatg ttatcgctgg aaaccagctg acgacgcagc gctacggcac
    19201 gacgcggcac attgagcgga ccaggcagca cgccttcagt gttcatcccg cgttcaatac
    19261 cgtcatgcat cacttgccag atacgggcaa aaccggcgtc aatttccgct ttgctacgca
    19321 gcgccagctc gttgtgcatc atcagaccag atatagacag gccgttgtaa tcacacattt
    19381 tcagcagttc acctgctgag tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca
    19441 ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttactta
    19501 atagcgcttc ctgtcccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca
    19561 tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc gcaccagatg
    19621 ccactggcag ccgcccgctg cgcgttacta actctataaa tgcagggatc tcatcaatga
    19681 caacatcctg cggactgttt cctgccagtc ccatgataat ggcgacatcc gtggcatggc
    19741 ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta
    19801 ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacgg
    19861 tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca
    19921 caccctcgga ttgccgttca gtgaagtgga gcggaacgac cttgcgaccg tcccgctcac
    19981 gaggctttac gcactacgta ctgcgatggc ttcaatttcc agcttcacat ctttcggcaa
    20041 acgcgcgacc tgtacacagc tccgggtcgg ataggtcgcc tgatgctcat cgaagaactg
    20101 cttatagact tcgttgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt
    20161 gatgatatcg cccacgctca gcccggcagc aaccacgatc gctttgacgt tttcgaggct
    20221 taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtttgtgggc aaaccggaat
    20281 ttgcccggag gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc
    20341 gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct
    20401 gggtaaaagt acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca
    20461 cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat
    20521 ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg
    20581 cttcgtggtg gaaataccca tccagcaggc cgacaaggtt ggttttacgt actggatctt
    20641 ctttgcccag cgccgcaggc acaatcgaga aggtgtacga gatcccgtct ttggcgtagg
    20701 tgaacggcag tttcgccacc gacgtcaatg aggccacggc acctttgcga tcacgaccat
    20761 gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc ggcgtattac
    20821 cggttttctg gccgtacacc acgttagaag tgatagtcag aatcgactgg gtagggacgg
    20881 cgttgcgata ggttggcagc gctttaattt tcttcataaa gcgttcaacc agatcgcagg
    20941 caatgctgtc tacgcgctcg tcgttgttac cgtactgcgg atattcgccg tcgatttcaa
    21001 agtccaccgc caggccgttt tcgtcacgga ttggtttcac gcgggcatat ttgatggcag
    21061 acagggagtc cgtcgccacc gacaggcccg cgatgccgca tgccatagtg cgatagacat
    21121 cacgatcgtg cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa
    21181 tgatattcag cgcactgatg tactgcaccg ccagccagtc catgaagtga tcgagactgt
    21241 ccatcacttt gtcgtaatcc agcacgtcgt ccatcaacgg tgctgttttc ggcccgacct
    21301 gaatcttcag cttctcgtcc accccgccgt taattgcgta cagcagtgtt ttcgccaggt
    21361 tagcgcgtgc accaaagaac tgcatttgct taccaatcac cattgggctg acgcagcagg
    21421 caatcgcgta atcgtcgctg ttgaagtcag tacgcatcag atcgtcgttt tcatactgca
    21481 aggaagaggt gacgatcgac acctgcgcgg catatttttt gaaggcaatc ggtaattctt
    21541 ccgaccaaag aatggtcagg ttaggttccg gtgcaggccc catagtgtgc agggtgtgca
    21601 aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc cagccccatc ccgccgatca
    21661 cttccgtcgc ccagattggg tcgccggaga acagcgaatc aaattccggt gtacgcaaga
    21721 agcgcaccat acggatcttc ataatgaagt ggtcgatcag ttcctgtgcc tgctgctcat
    21781 tgagtacgcc agctttaaag tcgcgctcaa tgtagatatc gaggaacgat gccgtgcgac
    21841 ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc caggtaagcg aagtagagcc
    21901 actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat atcaaagcca tatttcgccg
    21961 ccatttcctg aatctgcaac agcgcatgac gatgctctgc cagctcctca cgcagacgga
    22021 tggtggcttc cagatcctcg cctttttcca gacgagactg gagatcggca aattgcagtt
    22081 cgcgttcacg caccagataa ctgatgccat acagcgctac gcggcgatag tcaccgataa
    22141 tgcgcccacg accatagcca tctggtaaac cggtcagcac gccagattta cggcagcgca
    22201 gcatatccgg tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa
    22261 acagatattc aaattcactg tccatttcac gaccataggc gtggaatgaa cttttaatca
    22321 tattaatgcc accgaacggg tgtaacgcac gtttcaacgg cgcatccgtt tgcaggccaa
    22381 caattttttc cagcggctgg ttaatatatc ccgcatcatg agcggtaatg gtggtggcaa
    22441 tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc aatacgaatg ccttccatca
    22501 ccttttccca caattccgtg gtggctggcg tcgcttcagc gaggaaagat tcatcgcctt
    22561 catacggtgt atagttatgt tgaataaaat cgcggacatt aatttcgttt ttccagtccg
    22621 tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca atatctacct
    22681 tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct gcggacgcgt
    22741 tcactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgttg gttgggataa
    22801 cggcgcaaat gacacgggca ttttcactgg aaacaattcg ctctccacag gagttagagc
    22861 gattattcat ttctgtatca atctctacgc ctaatacagc caaatgttcc atgaccagac
    22921 gacgaattaa acttgaattt tctcctattc cgccggtgaa tataattcca tccaggcgac
    22981 gtaatgaggc tgcgtgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg
    23041 ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag
    23101 aaagaccgga aatacctaat aatcccgact ctttattcac tacgcgttcc aggtcaccca
    23161 ggctctggtt ggtttggctg gcgacccagg acatcgcacc aaagtcgaca tcaccactgc
    23221 gggtacccat catcaagcct tccagcggcg tcattcccat tgaagtgtca acactctgac
    23281 cgttgcaaac tgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt
    23341 catcttccgc cagattcagc aaagaatgcg cgcgctggga aacatagcgg tgcgacgtgc
    23401 cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc aggccgtata
    23461 aataagcttc cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta
    23521 cgccaggaaa taattgctgc gccgattcaa taccacttaa attggcgtaa ttatgcaggg
    23581 gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata atggcggact
    23641 cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaggccaca ctgtcattta
    23701 aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag
    23761 ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt
    23821 cggcaatacc tgacattaat acttcacagt cgctggcgtc gagtacggaa aacttaatcg
    23881 aagacgaacc
//
LOCUS       DAOTRK010000070        23766 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.070, whole genome shotgun sequence.
ACCESSION   DAOTRK010000070 DAOTRK010000000
VERSION     DAOTRK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.070"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(4..1056)
                     /gene="ompA"
                     /locus_tag="QIE41_002613"
     CDS             complement(4..1056)
                     /gene="ompA"
                     /locus_tag="QIE41_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDS3531489.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(1413..1922)
                     /gene="sulA"
                     /locus_tag="QIE41_002614"
     CDS             complement(1413..1922)
                     /gene="sulA"
                     /locus_tag="QIE41_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDS3531490.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVLASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            2141..2770
                     /gene="sxy"
                     /locus_tag="QIE41_002615"
     CDS             2141..2770
                     /gene="sxy"
                     /locus_tag="QIE41_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDS3531491.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(2733..4895)
                     /gene="yccS"
                     /locus_tag="QIE41_002616"
     CDS             complement(2733..4895)
                     /gene="yccS"
                     /locus_tag="QIE41_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDS3531492.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILTFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(4905..5351)
                     /locus_tag="QIE41_002617"
     CDS             complement(4905..5351)
                     /locus_tag="QIE41_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDS3531493.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            5474..7528
                     /gene="helD"
                     /locus_tag="QIE41_002618"
     CDS             5474..7528
                     /gene="helD"
                     /locus_tag="QIE41_002618"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDS3531494.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWRRWSSEMSEIASGV
                     LQQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWCKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLETTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(7560..8018)
                     /gene="mgsA"
                     /locus_tag="QIE41_002619"
     CDS             complement(7560..8018)
                     /gene="mgsA"
                     /locus_tag="QIE41_002619"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDS3531495.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(8114..8776)
                     /locus_tag="QIE41_002620"
     CDS             complement(8114..8776)
                     /locus_tag="QIE41_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDS3531496.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            8949..9362
                     /locus_tag="QIE41_002621"
     CDS             8949..9362
                     /locus_tag="QIE41_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDS3531497.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(9407..9724)
                     /gene="hspQ"
                     /locus_tag="QIE41_002622"
     CDS             complement(9407..9724)
                     /gene="hspQ"
                     /locus_tag="QIE41_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDS3531498.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(9782..10972)
                     /gene="rlmI"
                     /locus_tag="QIE41_002623"
     CDS             complement(9782..10972)
                     /gene="rlmI"
                     /locus_tag="QIE41_002623"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDS3531499.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWIFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            11067..11345
                     /gene="yccX"
                     /locus_tag="QIE41_002624"
     CDS             11067..11345
                     /gene="yccX"
                     /locus_tag="QIE41_002624"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDS3531500.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSEELTDFRIR"
     gene            complement(11342..11671)
                     /gene="tusE"
                     /locus_tag="QIE41_002625"
     CDS             complement(11342..11671)
                     /gene="tusE"
                     /locus_tag="QIE41_002625"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDS3531501.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(11762..12421)
                     /gene="yccA"
                     /locus_tag="QIE41_002626"
     CDS             complement(11762..12421)
                     /gene="yccA"
                     /locus_tag="QIE41_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDS3531502.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(12628..12715)
                     /locus_tag="QIE41_002627"
     tRNA            complement(12628..12715)
                     /locus_tag="QIE41_002627"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12679..12681),aa:Ser,seq:tga)
     gene            13142..14260
                     /gene="hyaA"
                     /locus_tag="QIE41_002628"
     CDS             13142..14260
                     /gene="hyaA"
                     /locus_tag="QIE41_002628"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDS3531503.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            14257..16050
                     /gene="hyaB"
                     /locus_tag="QIE41_002629"
     CDS             14257..16050
                     /gene="hyaB"
                     /locus_tag="QIE41_002629"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDS3531504.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELVSVQVR"
     gene            16069..16776
                     /gene="hyaC"
                     /locus_tag="QIE41_002630"
     CDS             16069..16776
                     /gene="hyaC"
                     /locus_tag="QIE41_002630"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDS3531505.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            16773..17360
                     /locus_tag="QIE41_002631"
     CDS             16773..17360
                     /locus_tag="QIE41_002631"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDS3531506.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKISLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            17357..17755
                     /gene="hyaE"
                     /locus_tag="QIE41_002632"
     CDS             17357..17755
                     /gene="hyaE"
                     /locus_tag="QIE41_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDS3531507.1"
                     /translation="MSNDTPFNALWQRMLARGWTPISECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            17752..18609
                     /locus_tag="QIE41_002633"
     CDS             17752..18609
                     /locus_tag="QIE41_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDS3531508.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLVLWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPS"
     gene            18743..20287
                     /gene="appC"
                     /locus_tag="QIE41_002634"
     CDS             18743..20287
                     /gene="appC"
                     /locus_tag="QIE41_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDS3531509.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQVAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            20299..21435
                     /gene="appB"
                     /locus_tag="QIE41_002635"
     CDS             20299..21435
                     /gene="appB"
                     /locus_tag="QIE41_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDS3531510.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGAIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            21448..21540
                     /gene="cbdX"
                     /locus_tag="QIE41_002636"
     CDS             21448..21540
                     /gene="cbdX"
                     /locus_tag="QIE41_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDS3531511.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            21620..22930
                     /gene="appA"
                     /locus_tag="QIE41_002637"
     CDS             21620..22930
                     /gene="appA"
                     /locus_tag="QIE41_002637"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDS3531512.1"
                     /translation="MKAILIPFLSLLIPFTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITIHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELEQVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(22918..23130)
                     /gene="cspH"
                     /locus_tag="QIE41_002638"
     CDS             complement(22918..23130)
                     /gene="cspH"
                     /locus_tag="QIE41_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDS3531513.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            23416..23628
                     /gene="cspG"
                     /locus_tag="QIE41_002639"
     CDS             23416..23628
                     /gene="cspG"
                     /locus_tag="QIE41_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDS3531514.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            23639..>23766
                     /locus_tag="QIE41_002640"
     CDS             23639..>23766
                     /locus_tag="QIE41_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDS3531515.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMIT"
BASE COUNT         5810 a         5960 c         6356 g         5640 t
ORIGIN      
        1 aacttaagcc tgcggctgag ttacaacgtc tttgatgcct ttaacttcga tctctacgcg
       61 acgatccgga gccaggcagt cgatcagtgc agcacgctgt ttcacgttgt cacaggtgtt
      121 gccagtaacc gggttggatt cgcccatacc acgtgcggag attttgtctg ccgggatacc
      181 tttagagatc aggtaatcaa caacagactg agcacggcgc tcggacagac cctggttgta
      241 agcgtcagaa ccgatgcggt cggtgtaacc cagaacaact acggaaccgt ctttcggatc
      301 caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg gtttcagggt
      361 tgctttgttg aaggtgaaca gaacgtcaga cttcagagtg aagtgcttgg tctgtacttc
      421 cggtgccgga gctggagccg gagcaactac tggagctgct tcgccctgac cgaaacggta
      481 ggaaacaccc aggctcagca tgccgttgtc cggacgagta ccgatggtgt gtgcgtcacc
      541 gatgttgttg gtccactggt attccagacg ggtagcgatt tcaggagtga tcgcatactc
      601 aacaccgcca gcgaagaccg gagaaacgcc ggtatcgtgg tctttaaaag atgcgccacc
      661 aggtacgtta gccttggtgt ctgcacgcca taccatacca cccagacgag tgtagatgtc
      721 cagatcgtca gtgattgggt aacccagttt agcggtcagc tgaacgccct gagctttgta
      781 tgcgccgttg atgttgtcgc ctttgtacgg catacgacct aaccagtcgt aacccatttc
      841 aaagccaaca tacgggttaa cctggtaacc accaaaagca cctgcaccca gttggttttc
      901 gtgggtcgga ccattgttag gaataaaacc agtgtcatgg tactgggacc agcccagttt
      961 agcaccagtg taccaggtgt tatctttcgg agcggcctgc gctacggtag cgaaaccagc
     1021 cagtgccact gcaatcgcga tagctgtctt tttcattttt tgcgcctcgt tatcatccaa
     1081 aatacgccat gaatatctcc aacgagataa cacggttaaa tccttcaccg ggggatctgc
     1141 tcaatattaa ctctaccgat atcttcggct tatgccgagc acccctggcg atgtaaagtc
     1201 tacaacgtag ttgaaaactt acaagtgtga actccgtcag gcatatgaaa aaaaagtctt
     1261 gtataaggta cgtttaatct tttttgtcag cgacaattta cagaagaaaa tcgcggaaac
     1321 cgcttcagac aagcctccgc aaggaaaatt agtcacgact gaaagcattg gctgggcgac
     1381 aaaaaaagtt ccaggattaa tcctaaattt acttaatgat acaaattaga gtgaattttt
     1441 agcccggaaa gttgtctcgt tgcgtgagag gatgcgctta ccggacgcat aataaacccc
     1501 atagcgttac cttcatttgc cgcatcaaca agttcagcat gctcttcttc agtcaaatca
     1561 tctgccaacc aaccgatcac cacactgtaa ttgcccgtgc gtaaagcgcg aaccattgac
     1621 tccacggtgt ggcaagggga gagctggcta atctgcatca ctttcgttaa gggtagccca
     1681 gatgccagaa cccattcccg actcagtttt tgttgcggtg ttaaccagag ttgccagcgc
     1741 gattgctgac cgagttgctg taacaatggc aacagtagaa gttgcgtcat catgggctga
     1801 tcttcgcgat agacaacttc actgataagc ccggctgtag tgttttccgt agagacacgc
     1861 gcaattttac ttgctgcgga tgagaacgac gaagaacgat gtgcatagcc tgaagtgtac
     1921 ataatcaatc cagcccctgt gagttactgt atggatgtac agtacatcca gtgacaacaa
     1981 agatcaaccc tattttcgga aagagcctcg caaattttgt cgttggtgac gggaaaacat
     2041 aaattaatct tgccccttaa gaataagttg cctattttcg tagttaacgg atccgttaat
     2101 gtgaatcatt cttttatgtt atgattttaa aaggaatttt atgaaaagcc tctcctataa
     2161 gcggatctat aaatcacaag aatacctggc aacgttgggc acaattgaat accgatcatt
     2221 gtttggtagt tacagcctga ccgttgacga cacggtgttt gcgatggttt ctgatggtga
     2281 gttgtatctt cgggcttgtg agcaaagtgc acagtactgt gtaaaacatc cgcctgtttg
     2341 gctgacatat aaaaagtgtg gccgatccgt taccctcaat tactatcggg ttgatgaaag
     2401 tctatggcga aatcaactga agctggtgcg tctgtcgaaa tattctctcg atgcagcgct
     2461 gaaagagaaa agcacgcgca atacccggga aagactgaaa gatttgccca atatgtcttt
     2521 tcatctggaa gcgattctcg gggaggtggg gattaaggat gtacgggcgt tacgtatact
     2581 tggggcaaaa atgtgttggt tgcgactgcg gcagcaaaac agtctggtga cagaaaagat
     2641 tctgtttatg cttgaagggg ccattatcgg cattcatgaa gctgcgctcc cggtggcacg
     2701 ccgccaggag cttgcagaat gggctgactc tcttacgccg aaacaggagt ttcctgcgga
     2761 acttgagtaa tctggcgttg cagacgacca atctctggca gtaatgcaat caataaccca
     2821 acttgttgta cgaccagagg ttctttgctg tctgcccgcg gttcaagttg ctgcatccgc
     2881 tgtttcaggc cagctaatgc ctgattgacg cgttcttcat cagcaggttg atgatgtagc
     2941 gcgtcatcaa catagcaaac tgcgtcatca agaaacgtca gaatttcagg attagttaac
     3001 tgctcccggt gagcaccgag ggctgagata tagctggtaa acgtatggtt aaggcacagc
     3061 aaccgaaacg cggcctcgcg aatttgcggg gtaacgttcg gctcgctgga catatttgat
     3121 accaccgacg ccagttcagc atcacggttg tgtgcatcgc ggcgggcaat acgatacgcc
     3181 agacggttat cacgcccctg atggtattgc tccagtatgg catcgagata gcgacagttg
     3241 gcctcggtgg cacgttcgag catgcgcggc agattgcgaa acttccagtc aggccagatg
     3301 tagctcaccg ccgcccacgc aatggcacaa ccaatcagcg tatcgattac gcgaggtaac
     3361 gccacttcaa aaccttcacc cagcaagtta aaacaaagta gcaccaaaag tgtgatgaac
     3421 atcgttgcat gggcgtattg cacgttacgg aaggcaaaaa agagcacgcc ggtgataacc
     3481 agtagcacca gttgcccttc cagtgacggt acaaaccaca gcacaggaat gccaatggcg
     3541 atacctacca gcgtaccaat aatccttaac ttcaggcggt ggcgcgtggc gttatagttt
     3601 ggctggcaga caaacaaact tgtcagcaag atccaatacc cgtgatgcat tccggttatc
     3661 tgaatgatgg cgtagccgaa gcacaacacc agcgacattc ttaccgcatg acggaagagg
     3721 gcggattccg gcgtgaagtg acggctaaga cgcagccaga tatcactcaa tccgtgcggg
     3781 ctgtcatcag cgagctcatt ttcgtcatta ttatggggca gtgcctgggc ctgttctgat
     3841 tcaattgttg ccagttgggc atcaatggcg cgtaaattgt tcagcaaaaa tcccagtgtt
     3901 ttgagtaaat cggcaggtgc gccgttatcg cgcatccgct ccagcgcagc atcaatatgc
     3961 gtaaaagcgc gctcgaaatg cggatcatgt tgataaggct gacgcaacaa aatacagcgt
     4021 gacagttgct ggcacgcctg gccctgcatc gacatcagcc gctgaaaacg gaacagcacg
     4081 tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa tatgagaaga gctggcacgc
     4141 tcgtgaatat cctgcgcgac gaagtaataa tgcagcgtgc gacgcgttcc ccgttgacca
     4201 cgatcgccac gtaagcgggt cagcagcgag agtttcgtct gattcaatgt cgccatcagc
     4261 tgaccgttgg cgagagccaa atcgtacagc ggtgcctggc tttcatcttc aatatcagga
     4321 tcaaacatgc gcgacttgag ctcaaggtaa cgcgccagtt gttcatagca acgcgccagg
     4381 ttgtcctgca gcgggcggac cgggaacagt agatgaccaa taagtgtcag gacgttgtac
     4441 cagacggcac cggccagcag atacatcggc tgctgatacc agtgctcata cagtgatgtt
     4501 cccaacatag tgtaaatggc gatcagcaat gcaccgaagg caattgttgc atagcgttga
     4561 cccagaccgc cgagcaaaat gaagccgctg gtagagagcg ttaagccaat cgcaaatagc
     4621 cagggccagg gaaacagcaa ttctactgag gccgaggcga taaaaaagca gaacagcgta
     4681 atgatgaggt tacgcaaacg tcccgccagt cggtcatcga gatcggtcag cgccgctgcc
     4741 accatcccca gcgttagcgg aatcgtcagt tttacatcac ccagccacca cggaaacgct
     4801 gtggttccac aaagcgcaat aaaaatacgc gcgtaataca gccaggcgct gttccaggta
     4861 tagcgtttga gcaaaggact tagcataaag gccatattag tggattattc aaaacgacga
     4921 cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg ataccacgcg acgaccgacc
     4981 ggccatagtg caatagcggc aattttaaag ttcgcaatgc cgacaggaat gccaatgatg
     5041 gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc ataaccacca gccaaagaaa
     5101 atcagccaga aaatattcaa taccgtaccg ccagtattta gcagcacatt tttgccagcc
     5161 gggttcagtt catcgacatg aatagcttca ttgccataag gcaccagaga cagtttagtg
     5221 atctcccagc aggatcgtgt cagcggtaag gtaaaaatca gcacaatact gaccagagtc
     5281 gccaacagcc agcccagagt ggtggcaaat ccgccaagca caaagttcag aatgttcaaa
     5341 acggtacgca taaaacctcg ctttactgtg gttttcagta atgggcggca attgtaacgt
     5401 ttttttgggc tggagcacgt tttctctgac ggttacactg ataagaaatc atttcgtgtg
     5461 gatctacaga gtcatggaac tgaaagcgac aacgcttgga aaacgtctgg cacagcaccc
     5521 ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc tccggcgatc gccacgaata
     5581 cctcattcct ttcaatcaat tactggcgat tcactgtaag cgcggtctgg tgtggggcga
     5641 gctggaattt gtactgccgg gcgaaaaagt ggtgcgtctg cacggtactg aatggggcga
     5701 gacgcagcgt ttttaccatc atcttgatgc tcactggcgg cggtggagta gcgagatgag
     5761 cgaaattgca tctggtgttt tacagcagca actggattta attgccacgc gcaccggaga
     5821 aaataaatgg ctgacgcgtg agcaaacctc tggcgtgcag caacaaatcc gccaggcttt
     5881 gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc gataactgcc gtgaggcgtg
     5941 gcgtaaatgt caggcctggt tgaaagatat tgaaagcgct cggttgcagc ataatcaggc
     6001 atataccgaa gccatgctta ccgagtatgc ggattttttc cgccaggtcg agtcttcacc
     6061 gctgaatccg gcgcaggccc gggcagtcgt taatggcgag cattctctgt tagtgctggc
     6121 aggtgcagga agcggaaaaa cgtcggtgct ggtagcccgt gcaggctggt tgctggcgcg
     6181 tggtgaagcg tcccctgagc aaattttatt gctggcgttt ggtcgcaaag ccgctgaaga
     6241 gatggacgag cgtattcgag aacgactaca taccgaagac attaccgcac gcacatttca
     6301 tgcgctggcg ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag tcagcaaact
     6361 ggaaaatgat accgctgccc gtcatgaact ttttattgct gagtggtgca agcaatgcag
     6421 cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg acggaagaaa tgcagtggtc
     6481 agtgccagaa ggtaacttct gggatgatga aaaattacag cgtcgtcttg cctcacgcct
     6541 cgatcgttgg gtaagtctga tgcggatgca cggtggtgca caggcagaaa tgattgccag
     6601 tgcacccgaa gagattcgcg atctgttcag taaacgtatc aagttgatgg ctccgttatt
     6661 aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg gccttattca
     6721 ccaggcgatt gtgattctgg agaaaggtcg ctttatcagc ccgtggaagc atattctggt
     6781 tgatgaattt caggatatct cgccgcagcg cgcagcgttg ttagcggcat tacgcaagca
     6841 aaacagtcag acgacgttgt tcgccgttgg tgatgactgg caggcgattt atcgcttcag
     6901 cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag gtgatcgctg
     6961 cgatttagag acgacttacc gttttaacag tcgtatcggt gaggtggcaa accggtttat
     7021 tcagcagaac ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg gagacaaaaa
     7081 agccgtcacg ttattggatg agagccatct tgacgctttg ctggataagc tctctggtta
     7141 tgccaaaccg gaagagcgca ttctgatcct ggcgcgttac catcacatga ggcctgccag
     7201 cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc gactttatga ccattcatgc
     7261 cagcaaaggg caacaggcgg attacgtcat catcgttggc ttgcaggagg gaagtgatgg
     7321 ttttccggct gcggcgcggg agtcgattat ggaagaggcg ctactgccac cggttgagga
     7381 tttcccggac gctgaagaac ggcggttaat gtacgtggcg ctgacccggg cacgccatcg
     7441 ggtatgggca ctgtttaaca aagagaatcc ctctcccttt gtggaaatac tgaaaaatct
     7501 ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc tgtgcaatat
     7561 tacttcagac ggtccgcgag ataacgctga taatcgggga tcagaatatc gaccgcgtcg
     7621 ttgaaatgcg gcgactggat tatgaagtct gccgttgcca cgttggtggc gaccggaatg
     7681 ttccataccg tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg cacggcattt
     7741 agtggatccc agaagaaaat caatacatca attttccctt ctgagatcaa tgcgccaacc
     7801 tgctggtcac cccccattgg gccactcaac atcgcgttga cgttcatgcc ggtcgcgcgg
     7861 gaaattaagt taccggtagt gcctgttgca tacagtacgt gttgttccag taacggttga
     7921 tgccgttcca cccagctcat cagcatttgt ttgcagtgat cgtgtgccac cagcgcaata
     7981 tgtttccgcg caggtaaagt gcgagtcgtc agttccataa tgtacatccg tagttaactt
     8041 tcctacagat tactgtaagc acttatcgct gcaagataaa gaccgaaaaa gcctgcgcac
     8101 aggcacaaaa atctcaggaa gatggttgtt tttccgccca ttgcaggaaa gtatttcgcg
     8161 tttgtgggtc agccagttta aaccaatact tcagccgttg ttctgtgagc acctgagact
     8221 gcggtggaat agcatccagc tcggaaaccc ccgaaagcag cgtactgtca tctgccatca
     8281 tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt tcaacctcga
     8341 cctcataatc aatcgttttt gccgttgagg taatggcgag aatatccagc tttaccggaa
     8401 tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc agcatcaaaa tggttagctt
     8461 cccgctcatt ctccagacga ggcaggcgaa aattgacctg gttgatgagc tgggtattaa
     8521 aactgaccac cagcggtggg gagatataca gccgttcttc actattggaa agatgaatcg
     8581 tcttctcaac gcgaaacact aactgatgcg gtccgttatc cagttcaatg ctgtccgcac
     8641 cgcgcagcag agagctggaa acctttttac cgtcgagtac cagaagatcg acatcggttg
     8701 aaagccgcag cgtggtggca aaaacggata ccggcaaaca taatgcaatc aaggtggtca
     8761 cgatgccggt tttcataggt agctcctgtc aaaagaccgc ccgaataata atgtgtcaaa
     8821 atttttcttt aatcatactt actaacatat agacatattt cccgcttttg ctctcattca
     8881 ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta cactttgtta aacagtctgg
     8941 aggaaataat gaaagaaacc gatattgcag gcattttaac gtccactcac accattgcgc
     9001 tggtgggggc aagcgacaaa cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg
     9061 accaggggta tcacgtcatt ccggtttcgc caaaagttgc tggcaaaaca ctgctgggcc
     9121 agcaggggta tggcacgctg gcagaagttc ctgaaaaagt cgatatggtc gatgttttcc
     9181 ggaattcaga agcagcgtgg ggcgtagcgc aggaagcgat cgccatcggc gcgaaaaccc
     9241 tctggatgca actgggggtg attaatgagc aagcggcagt actggcacgg gatgccggat
     9301 taaatgtagt gatggatcgc tgcccggcta ttgagatccc tcgcctgggc ctggccaaat
     9361 aaaaaatccc cggaaggcaa aaaccttccg gggatttgtt cagggattag ttacgcagac
     9421 gcggggcctg gagttgtttg cggatggtct gcgccagctc atccatggat ggctgttcag
     9481 gatgttcatc ctgcaattcg ctacttagct gggcttcagc caggtaggta tgaaccggta
     9541 ggccgttatc gtcctccatc accacgtgat accacggagc ggcgcgaagc tcgtcattca
     9601 ccgccagctc atcaggcgac ggttcagaga gcgaataaac cgggtcgata tccacgacca
     9661 ctccgagata acctaacagg gaatggcgga cctgctggcc gataccgaat ttgctggcaa
     9721 tcatagtcac ctcccgggaa atctttacct ttgatataag ggtaaaattc cacatttcaa
     9781 gttacatgac gcgacaggca aaccctttca gatatagccc ttccggatag gtagcgatca
     9841 ccggatgatc ggctgcctga cggaactgct ctataaattg tacatcacga ccggcatcaa
     9901 ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt catcaggcca gaacaggaga
     9961 aagtcaggag aactccgcct tcattcagca actgaatcgc cagcatgttg atgtctttat
    10021 agccacgaca cgcgcccatc aactggcttt tattctcaac aaacttcggc gggtccatca
    10081 cgataacgtc aaatttttca ccgcgatcgc gataagtacg cagcaattta aagacatcat
    10141 cacggacaaa ctcagccttg ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa
    10201 tatccagcgc ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg cccatcagtg
    10261 ccgatacggc gaaaccaccg gtataggaga aacagttcag cacacgttta ttttcaacgt
    10321 agcggcgggt agccaggcgg ctgtcacgct ggtccaggta gtagcccgtt ttgtgcccgt
    10381 gctgtatgtc caccagcagc ttcatgccgt gttcttcaat cggcagcagg gcaggcggta
    10441 gctctccggt aacggggccc tgggtcagtt ccatcccttc ttttttacgt accgcaacgt
    10501 cgctgcgatc gtaaatcgca cattccgggt acagcgtttg cagggcacta attaatgctg
    10561 cgcgctgata ttctgcgcca gcactcagca attgcagcac cagaaaatta ccgaaacgat
    10621 cgatagtaat acccggcagg ccatcagatt caccggcgat taaacgatag ctgtcgaggc
    10681 catctttttg cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg cgggaaaaaa
    10741 aagcaatgtc gatagattca ttcgggtcaa acgtccagac gcgcgcccgg atttgcgaag
    10801 ctggcgaata agcgccgcgt gctaaccatt ttccctgatg atcaacaata tcgatggttt
    10861 ccccgaggct ggctttacct tccatgcggg caacggcccc ggaaaagatc cacggatggc
    10921 gacgaagtaa tgatttttcg cgccctttgg ctaacactaa acgtacactc ataatttact
    10981 tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga tttgcaacgc
    11041 gtccattaag gataaggaga gcagcaatgt cgaaagtctg cataattgcc tgggtttacg
    11101 ggcgagttca gggcgttgga tttcgctata ccacgcagta cgaagcgaaa aaactgggat
    11161 tagccgggta cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag
    11221 aagggcaggt agaaaaatta atgcagtggt taaagagtgg cggcccgcgt tctgcgcgtg
    11281 tagaacgggt gctcagcgag ccgcatcatc cctcggagga attaaccgat tttcgtatcc
    11341 gttaaataca tttaaccggt tttggcaggc cagcaatttt ggtggcttgc ttagccggac
    11401 ctttcggaaa cagtcggtat aaatagcggc tgttaccttt ttcttcgcca aatttattcg
    11461 ccatcgcttt taccagcata cgaatcgccg gagaagtatt gaattccaga tagaaatcac
    11521 gcacaaaacg caccacttcc cagtgttctg gcgacagcga aatcccttcg ttctctgcaa
    11581 tcaccactgc cagcggctca ctccactggc tgctttcttt gagatagcct tcggtatccg
    11641 tttctatctc tttaccttcg aagatcagca taattattac taccttaatc agactggcgg
    11701 cagtgtaaca aaaaacaaag ccccgcataa agcgaggctc ggtaagtaac cctgggcgag
    11761 attaatcgcg gctggcgaag cccagaatgc tcagcaggct gacgaagatg ttgtacagcg
    11821 aaacatacag gctaaccgtg gcacgaatat agttcgtttc accgccatga atgatgttgc
    11881 tggtttcaaa caaaatagcg ccagaggaga tcagaatgaa gaccgcgctg atcgccagat
    11941 gcagagcagg cagttgcagg aagatattag caaccatacc aatcagcacc accacaatac
    12001 ccgccatcag cataccgccg aggaacgaca tatctttgcg ggtggtcagc acatatgcag
    12061 agcagcagaa gaacactaac gccgttccgc ccagtgccat agcgattacg tcacccattc
    12121 cggcagacag ataggtattc agaattggtc cgaggatata acccagaaaa ccggtaaagg
    12181 cgaatgcgga gataatcccg gtcggcttat tcgccgtttt ataggtcagg aacatcaaac
    12241 catacatacc caccagcgtc agaatcagac ccggagatgg cagcatcagc accgtactgg
    12301 cagttgcggt aatcgcagaa aaggccagcg tcaggctcag cagaaaatag gtattacgca
    12361 gcaccttatg ggtgctaagc agcgatgtac ggtcatgtga agaactaaca atacgatcca
    12421 ttgagtcact ctctatgaca gatgtaatta attaagcagc ataatgataa tgcgtaaggg
    12481 cacccagaag ttttacccat ctttacgcat ttgatctgga acaggtttaa cagcggatta
    12541 tcaggtcatt aagcaaatat aacgccctga gaatttcgac aggcaaaaga aaaaggggtt
    12601 agcatttagc taacccctta tcttatttgg cggaagcgca gagattcgaa ctctggaacc
    12661 ctttcgggtc gccggttttc aagaccggtg ccttcaaccg ctcggccaca cttccggaat
    12721 gacgcgcact ataaacatcc cgatgcggcg tgtaaacccc taatttgttt gtttgcctga
    12781 aaaacagaca aaagtgcatt gatagcgtga aataacagca gattgatcat ttcatcacca
    12841 tgaattcctt ctcttttact cgtttagcaa ccggctaaac atccccactg cccggcaaaa
    12901 agaaaaatag gtccattttt atcgctaaaa gataaatcca tacagtttgt attgttttgt
    12961 gcaaaagttt cactacgctt tattaacaat actttctggc gacgtgcgcc agtgcagaag
    13021 gatgagcttt cgttttcagc atctcacgtg aagcgatggt ttgccttgct acagggacgt
    13081 cgcttgccga ccataagcgc ccggtgtcct gccggtgtcg caaggagaag agacgtgcga
    13141 tatgaataac gaggaaacat tttaccaggc catgcggcgt cagggcgtta cccggcgcag
    13201 ctttctcaaa tattgtagtc tggctgccac gtcgctgggc ttaggcgcgg gaatggcacc
    13261 aaagattgcc tgggcgctgg agaacaaacc gcgcattccg gtggtatgga tccacggtct
    13321 ggaatgcacc tgctgtaccg aatcttttat ccgctccgct cacccgctgg cgaaggacgt
    13381 catcctttcc ctgatttccc tcgattatga cgatactttg atggctgccg ccggaaccca
    13441 ggcggaagaa gtctttgaag acatcatcac gcaatacaat ggcaaatata tcctcgcagt
    13501 agaaggtaat ccgccgctgg gcgagcaggg gatgttctgt atcagcagcg gacgaccgtt
    13561 tattgagaaa ctcaaacgtg ccgctgccgg agccagtgcg attatcgcct ggggaacctg
    13621 cgcgtcctgg ggctgcgtgc aggccgcgcg acccaatccg acgcaggcaa cgcctatcga
    13681 caaagtcatc accgacaaac ctattatcaa agtacctggc tgcccgccga tcccggatgt
    13741 gatgagcgcc atcattactt acatggtgac ctttgatcgc ttgccagatg tcgacagaat
    13801 gggccgtcca ctgatgtttt atggtcagcg tatccacgat aaatgttatc gccgcgctca
    13861 cttcgacgcc ggagagttcg tccagagttg ggatgatgac gctgcccgca aaggttactg
    13921 cctgtacaaa atgggctgca aagggcctac cacctataac gcctgttcct ccacacgctg
    13981 gaacgatggc gtttccttcc cgatccagtc aggtcacggc tgcctgggtt gtgcggaaaa
    14041 tggtttctgg gatcgcggtt cgttctacag ccgcgtggtc gatattccac aaatgggtac
    14101 tcattccacc gccgacaccg tcggtttaac cgcgcttggc gtggtggcag cggctgttgg
    14161 tgtgcacgca gtcgccagcg ccgttgacca gcgcagacgt cataaccagc aacctacaga
    14221 aaccgaacat cagccaggca atgaggataa acaggcatga gcactcagta cgaaactcag
    14281 ggatacacca tcaataatgc cggacgccgc ctggtggtcg acccgattac gcgcattgaa
    14341 ggccacatgc gctgcgaagt gaatattaac gatcagaatg tgatcaccaa tgccgtctcc
    14401 tgcggcacca tgtttcgcgg gctggagatc atcctgcaag ggcgtgaccc gcgcgatgcg
    14461 tgggcgttcg ttgaacgtat ctgcggcgtc tgtaccggcg tacacgctct ggcttcggtc
    14521 tatgccatcg aagatgctat cggcattaaa gtgccggaca acgctaatat catccgcaac
    14581 attatgctgg cgacgctctg gtgccatgac catctggtgc acttttatca gcttgccggg
    14641 atggactgga tcgatgtgtt agatgcgctg aaagccgacc cgcggaaaac ctccgaactg
    14701 gcgcaaagtc tctcctcttg gccgaaatca tcccctggct atttcttcga cgtacaaaat
    14761 cgcctgaaga aattcgttga aggcggccag ttggggatct tccgcaatgg ctactggggg
    14821 cacccgcagt acaaactgcc gccagaagcc aacctgatgg gctttgccca ctatctcgaa
    14881 gctctcgatt tccagcgtga aattgtcaaa atccacgctg tctttggcgg taaaaacccg
    14941 catccaaact ggattgtcgg cgggatgcct tgcgccatta acattgacga aagcggcgcg
    15001 gtcggggcgg tcaatatgga acgcctgaac ctggtgcagt caatcattac ccgcacggcg
    15061 gatttcatta acaacgtgat gatccccgac gccttagcca ttggtcagtt caacaaaccg
    15121 tggagcgaaa tcggcacggg tctttctgat aaatgcgttc tcagttacgg cgcgttcccg
    15181 gatatcgcca acgactttgg cgagaaaagt ctgctgatgc ctggcggcgc ggtgattaac
    15241 ggcgacttca acaatgtgct gccagtggat ttggttgatc cgcagcaggt gcaggagttt
    15301 gtcgaccacg cctggtatcg atatcccaac gatcaggtcg ggcgtcatcc gttcgatggc
    15361 atcaccgacc cgtggtacaa ccccggcgat gtcaaaggca gcgataccaa cattcagcag
    15421 ctgaatgaac aggaacgcta ctcgtggatt aaagcgccac gctggcgcgg taacgcgatg
    15481 gaagtggggc cgctggcacg cacgttaatc gcttatcaca aaggcgatgc tgcgaccgtt
    15541 gagtcggtcg atcgcatgat gtcggcgttg aaactgccgc tttccggcat ccagtcaacg
    15601 ttgggccgca ttttgtgtcg cgcgcacgaa gcgcagtggg ccgcaggtaa gttgcagtat
    15661 ttcttcgata agctgatgac caatctgaaa aacggcaatc tcgccactgc ttccactgaa
    15721 aaatgggaac ctacaacctg gccgacagag tgccgtggcg tcggctttac cgaagcgccg
    15781 cgcggggcgt taggccactg ggctgccatt cgcgatggca agattgatct ctatcagtgc
    15841 gtggtgccga ccacctggaa cgccagcccg cgcgatccta aagggcaaat tggcgcttat
    15901 gaagcggcgc tgatgaacac caaaatggcg atccccgagc aaccgctgga gatcctgcgt
    15961 actctgcaca gctttgatcc gtgcctcgcc tgttcaacac acgtgctggg cgacgacggt
    16021 agcgagctgg tctccgtgca ggtgcgttaa cagcgaagga gaatcatcat gcaacagaaa
    16081 agcgacaacg ttgtcagtca ctatgtcttt gaagcacccg tgcggatatg gcactggctg
    16141 acggtgttat gcatggcggt gttgatggtc accggttact ttatcggcaa gccgctacct
    16201 tccgtcagcg gcgaggcgac gtatctgttc tatatgggct acatcaggtt aattcacttc
    16261 agcgccggga tgatttttac cgtggtcttg ctgatgcgga tctactgggc ttttgttggc
    16321 aatcgttact cccgcgagct gtttatcgtg ccggtatggc gtaaaagctg gtggcagggc
    16381 gtgtggtatg aaatccgctg gtatctgttt ctggcaaaac gtccgagtgc cgatataggc
    16441 cataacccca tcgcccaggc ggcgatgttc ggctatttcc tgatgtcagt ttttatgatc
    16501 atcaccggct ttgcgctgta cagcgaacac agccagtacg ctatttttgc gccgttccgt
    16561 tatgtggtgg aatttttcta ctggacgggc ggcaactcaa tggacgttca cagctggcat
    16621 cggctgggga tgtggctgat tggcgcgttt gtgatcggtc atgtctacat ggcgctgcgt
    16681 gaagatatca tgtccgacga cacggtgatc tccaccatgg tcaacggcta ccgtagccac
    16741 aaatttggca aaataagtaa caaggagcgt tcatgagcga gcaacgcgtg gtggtaatgg
    16801 ggctgggcaa cctgctgtgg gccgatgaag gcttcggcgt gcgggtggcg gaacggctgt
    16861 atgcccatta ccactggccc gagtatgtgg agattgtcga tggcggtact cagggactga
    16921 acttgctggg gtatgtcgaa agcgccagtc acttgttgat cctcgatgcc attgactacg
    16981 ggctggaacc aggaacgctg cgaacctatg ccggagaacg cattccggct tatctcagcg
    17041 cgaagaaaat aagcctgcac cagaacagct tctccgaagt gctggcgctg gcggatatac
    17101 gtggtcattt gccagcacat attgccctcg tcggtctgca acccgcaatg ctcgacgact
    17161 acggcggtag cctgagcgaa ctggcgcggg agcaactgcc cgctgcggaa caatcggcgc
    17221 tggcgcagct ggctgcgtgg ggaattgtgc cgcaaccggc taatgaatcg cgctgtctca
    17281 attatgactg tctgtcgatg gaaaattacg aaggcgttcg cttgcgtcag tacaggatga
    17341 ggctggagga acagggatga gcaacgacac accatttaat gcgttgtggc aacgaatgct
    17401 ggcgcgtggc tggacgccga tcagtgaatg ccgtctcgac gactggctta cgcaagcgcc
    17461 agacggcgtg gtgttattaa gcagtgaccc gaaacgcacg ccagaagtca gcgataaccc
    17521 ggtcatgatt ggcgaattac tgcgcgagtt tcccgactat acatggcagg tagcgattgc
    17581 tgaccttgag cagagcgaag ccatcggcga tcgctttggc gtctttcgct ttcccgccac
    17641 attagtattt accggcggaa actatcgcgg cgtgctgaat ggtattcacc cgtgggcgga
    17701 actgataaac ctgatgcgcg gacttgtcga accgcagcag gagcgtgcct catgagcgaa
    17761 acttttttcc atctgctggg gccaggaacg caaccgaacg atgacagttt cagcatgaat
    17821 ccactgccga tcacctgtca ggtgaatgat gaaccgagta tggcggccct ggagcaatgt
    17881 gctcacagcc cgcaggtgat tgcgctatta aacgagttac aacatcaact aagcgagcgc
    17941 cagccgccgc tgggcgaggt gctggcagtc gatctgttaa atctcaacgc cgacgatcgt
    18001 cactttatca atacgcttct cggggaaggg gaagtgtcag tgcgcattca gcaggctgac
    18061 gacagtgaaa gtgaaataca ggaggcgatc ttctgcggat tatggcgggt gcgcagacgt
    18121 cgcggcgaca agttgctgga ggacaaactg gaggctggct gcgcgccgct ggtattgtgg
    18181 caggcggcaa cgcaaaacgt cttgccgaca gattcgctgt taccgccgcc cattgatggc
    18241 ctgatgaatg gcctaccgtt ggcgcatgag ttactggcgc atgtacgtaa ccccgacgcg
    18301 cagccgcaca gcattaatct gacgcaatta cccatcagcg aggctgatcg gctttttctc
    18361 tcacgtctct gtgggccggg aaatattcag attcgtacca ttggctatgg cgagagctat
    18421 atcaacgcca cggggttacg ccatgtctgg catttacgct gtacggacac cttaaaaggc
    18481 ccgttactgg aaagttatga aatctgccca ataccggaag tggtgctggc agcgccagaa
    18541 gatttggtcg actctgcgca gcggcttagt gaggtatgtc agtggctggc ggaaggggca
    18601 ccgtcgtaaa aagacggtaa gtatagcttt caggcttatg aatatcgcaa tcggcgaata
    18661 cctctggtcg tagagtttca ggataaagag ggagatctac cattaacggg ttatttttct
    18721 ctcttcgcct acaggagtgc gcatgtggga tgtcattgat ttatcgcgct ggcagtttgc
    18781 tctgaccgcg ctgtatcact ttttatttgt accccttacc ctggggctga tttttttgct
    18841 ggctatcatg gaaaccattt acgtggtcac cggcaaaaca atctaccgcg atatgacgcg
    18901 cttctggggt aagctcttcg gtatcaactt tgctcttggc gtggctaccg gcctgacgat
    18961 ggagtttcag tttggtacta actggtcatt ctattccaac tatgtgggcg atatttttgg
    19021 cgcaccgctg gcaatggaag cattaatggc cttcttcctc gagtccacct ttgtcgggct
    19081 gttcttcttc ggctggcagc ggctgaataa ataccagcac ctgctggtga cgtggctggt
    19141 ggcgttcggt tcaaatctct ctgcgttgtg gatattgaat gccaacggtt ggatgcaata
    19201 cccgaccggt gcgcattttg atatcgacac ccttcgtatg gaaatgacca gcttcagcga
    19261 gctggtcttt aacccggtca gccaggtgaa atttgtgcac accgtaatgg cgggctacgt
    19321 gaccggggcc atgtttatta tggcgatcag cgcctggtat ttactgcgcg gacgggagcg
    19381 cgatgtcgca ttacgctcgt ttgccatcgg ttccgtcttc ggtactctgg cgattatcgg
    19441 tacgctgcaa ctcggagaca gttcggcgta tgaagtcgcg caagtacaac cggttaaact
    19501 ggcggcaatg gaaggggagt ggcaaacgga acctgcacct gcaccgttcc atgtggttgc
    19561 ctggccggaa caggatcaag agcgtaacgc ctttgccatc aaaattcccg cgctgctagg
    19621 gatcctcgcc actcactcat tagataaacc cgtgccgggt ctgaagaatt tgatggctga
    19681 aacctaccca cgcttgcaac gcggacgtat ggcctggctg ttaatgcagg aaatatcgca
    19741 gggcaatcgt gagccgcatg tgttgcaggc attccgggag ctggaaggcg accttggtta
    19801 cggcatgttg ctctcccgct atgcgccgga tatgaatcat gtcacagccg cacagtacca
    19861 ggtggcgatg cgtggcgcga tacctcaggt tgcgccggta ttctggagtt tccgcatcat
    19921 ggtgggctgt ggttccctgc tgctactggt gatgctgatt gcgcttgtcc agacgctgcg
    19981 tggcaaaatc gaccagcatc gctgggtgct gaaaatggcg ctctggagtt tgccgctgcc
    20041 gtggattgcg attgaagccg ggtggtttat gaccgagttt ggtcgtcagc cgtgggcgat
    20101 acaggacatc ttaccgacat actccgcgca ctccgcctta accacaggac aactggcttt
    20161 ctcactgatc atgatcgtag ggctttacac cctgttctta atcgccgaag tctacctgat
    20221 gcagaaatat gcccgtctgg ggccgagcgc gatgcagagt gaacaaccga cgcagcaaca
    20281 ggggtaaagg agaaaatcat gtttgattat gaaacattgc gcttcatctg gtggctgctg
    20341 attggcgtga tcctggtagt ctttatgatc tccgatggat ttgacatggg gatcggctgt
    20401 ctgctgccgc tggtggcgcg taatgatgat gaacgtcgga tagtgataaa cagcgttggc
    20461 gcgcactggg aaggcaatca ggtctggtta atcctcgcag gtggggcatt atttgccgcc
    20521 tggcccagag tgtatgcagc ggcgttttcc ggcttttatg tggcgatgat cctggtgctg
    20581 tgctcgctgt tcttccgccc gctggccttt gattatcgcg gaaaaattgc cgatgcacgc
    20641 tggcgtaaaa tgtgggatgc cggtctggtc atcggcagtc tggtgccgcc ggtagtcttc
    20701 ggtattgcct tcggcaactt gctgctcggc gtgccgtttg ccttcacacc gcaattacgc
    20761 gtggaatatc tcggcagctt ttggcaactg ctgacgccat tccctttatt gtgcggattg
    20821 ctcagccttg ggatggtgat tttgcaaggt ggcgtctggt tacaactaaa aactgttggt
    20881 gcgattcatc tgcgttcaca gctggcgacc aaacgcgccg cactgctggt gatgctctgc
    20941 tttttgctgg cgggttactg gctgtgggtc ggtattgatg gctttgtact gctcgcccag
    21001 gatgctaacg gtccttccaa tccgttaatg aaactggtgg cagtgctacc tggtgcctgg
    21061 atgaataatt tcgtcgaatc gcccgttctg tggatcttcc cgctgctggg gttcttctgc
    21121 ccattgctga cggtgatggc gatttatcgt ggtcgcccgg gttggggatt tttgatggcg
    21181 tcattgatgc aatttggcgt gattttcacg gcaggcatca cgctgttccc ctttgtcatg
    21241 ccgtcaagcg tgagtccgat ctccagcctg acgttgtggg acagtacttc cagtcagctg
    21301 acgctgagca tcatgttggt aatcgtgctg atatttttgc ccattgtgtt gctctacact
    21361 ctctggagct actacaaaat gtgggggcgc atgacaacag aaactctccg ccgtaacgaa
    21421 aacgagttgt actaaggagc aggaacaatg tggtatttac tttggttcgt cggcattttg
    21481 ttgatgtgtt cgctctccac ccttgtgttg gtatggctgg acccgcgtct gaaaagttaa
    21541 cgaacgtaag cctgatgcgg cgcattagcg tcgcatcagg caatcaataa tgtcggatat
    21601 gaaaagcgga aacatatcga tgaaagcgat cttgatccca ttcttatctc ttctgattcc
    21661 gttcaccccg caatctgcat tcgctcagag tgagccggag ctgaagctgg aaagtgtggt
    21721 gattgtcagt cgtcatggtg tgcgtgcccc aaccaaggcc acgcaactga tgcaggatgt
    21781 caccccagac gcatggccaa catggccggt aaaactgggg tggctgacac cgcgcggtgg
    21841 tgagctaatc gcttatctcg gacattacca acgccagcgt ctggtggctg acggattgct
    21901 ggcgaaaaag ggctgcccgc agcctggtca ggtcgcgatt attgctgatg tcgacgagcg
    21961 tacccgtaaa acaggcgaag ccttcgccgc cgggctggca cctgactgtg caataaccat
    22021 acatacccag gcagatacgt ccagtcccga tccgttattt aatcctctaa aaactggcgt
    22081 ttgccaactg gataacgcga atgtgactga cgcgatcctc agcagggcag gagggtcaat
    22141 tgctgacttt accgggcatc gacaaacggc gtttcgcgaa ctggaacagg tgcttaattt
    22201 cccacaatca aacttgtgcc ttaaccgtga gaaacaggac gaaagctgtt cattaacgca
    22261 ggcattacca tcggaactca aggtgagcgc cgacaatgtc tcattaaccg gtgcggtaag
    22321 cctcgcatca atgctgacgg aaatatttct cctgcaacaa gcacagggaa tgccggagcc
    22381 ggggtgggga aggatcactg attcacacca gtggaacacg ttgctaagtt tgcataacgc
    22441 gcaattttat ttactacaac gcacgccaga ggttgcccgc agccgcgcca ccccgttgtt
    22501 ggatttgatc atgacagcgt tgacgcccca tccaccgcaa aaacaggcgt atggtgtgac
    22561 attacccact tcagtgctgt ttatcgccgg acacgatact aatctggcaa atctcggcgg
    22621 cgcactggag ctcaactgga cacttccagg tcagccggat aacacgccgc cagggggtga
    22681 actggtgttt gaacgctggc gtcggctcag cgataacagc cagtggattc aggtttcgct
    22741 ggtcttccag actttacagc agatgcgtga taaaacgccg ctgtcattaa atacgccgcc
    22801 cggagaggtg aaactgaccc tggcaggatg tgaagagcga aatgcgcagg gcatgtgttc
    22861 gttggccggt tttacgcaaa tcgtgaatga agcacgcata ccagcgtgcg ctctccatca
    22921 agacaaataa acatttgccg ctgttggtcc tcgcaggcca tttacgcggc aaaattccac
    22981 gcgtaatcct ggtataagca cttctgcgtc gcggggagtg aatgcggaaa tatggacctg
    23041 gacttcttta cgaccgtcgg aggggataat gaatcctttg ccgcttttgc gatcaaaggt
    23101 tttgacaatt cctgtcattt tacgggacaa acaaattcct tactgaatat actgcgctgc
    23161 actatacggg gttaataaaa taaagccagc gatatttaag accgccggac ggctaaaata
    23221 aaatttgctt aatctcaatt atcatgcgtt aatagctgcg tcggtttgaa agacagacag
    23281 catacaaagt agtttactaa agcagttctc attatcaggc attatcccct tcttttgagt
    23341 ctctctcctg aacactaagt agtttctgta ttaaagccct gtttgccgaa aggcccaaaa
    23401 tgaaggaagt aaaatatgtc taataaaatg actggtttag taaaatggtt taacgcagat
    23461 aaaggttttg gctttatcac tcctgatgat ggcagcaaag acgttttcgt ccatttcacc
    23521 gccatccaga gcaatgaatt ccgcacgctg aacgaaaatc agaaagttga attttctatt
    23581 gagcaggggc aacgtggtcc cgcggcagcg aacgttgtta cgctctaagg ttgccgttat
    23641 tactcaacat ctccatttcc gctgtccatg ttgtcatggt tcacagtacc gcacatcggc
    23701 attcgatgtg acggagcgaa atcctttagg ggcaaagtgt attttttgta aatcgacgat
    23761 gatcac
//
LOCUS       DAOTRK010000071        23640 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.071, whole genome shotgun sequence.
ACCESSION   DAOTRK010000071 DAOTRK010000000
VERSION     DAOTRK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.071"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..457
                     /gene="agaB"
                     /locus_tag="QIE41_002641"
     CDS             <1..457
                     /gene="agaB"
                     /locus_tag="QIE41_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDS3531516.1"
                     /translation="LTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDIQQKLMG
                     ITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKDVNVGN
                     MHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            496..1299
                     /gene="agaC"
                     /locus_tag="QIE41_002642"
     CDS             496..1299
                     /gene="agaC"
                     /locus_tag="QIE41_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDS3531517.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1289..2080
                     /gene="agaD"
                     /locus_tag="QIE41_002643"
     CDS             1289..2080
                     /gene="agaD"
                     /locus_tag="QIE41_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDS3531518.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2081..2848
                     /locus_tag="QIE41_002644"
     CDS             2081..2848
                     /locus_tag="QIE41_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDS3531519.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSHLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGENLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATDRFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(2898..3761)
                     /gene="rsmI"
                     /locus_tag="QIE41_002645"
     CDS             complement(2898..3761)
                     /gene="rsmI"
                     /locus_tag="QIE41_002645"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDS3531520.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3826..5862
                     /gene="lpoA"
                     /locus_tag="QIE41_002646"
     CDS             3826..5862
                     /gene="lpoA"
                     /locus_tag="QIE41_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDS3531521.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRIWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPAATTAAVSAPANPSAELKIYDTSSQPLSQILNQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGIAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5820..6215
                     /locus_tag="QIE41_002647"
     CDS             5820..6215
                     /locus_tag="QIE41_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDS3531522.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6235..6825
                     /gene="diaA"
                     /locus_tag="QIE41_002648"
     CDS             6235..6825
                     /gene="diaA"
                     /locus_tag="QIE41_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDS3531523.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6835..7410
                     /gene="yraP"
                     /locus_tag="QIE41_002649"
     CDS             6835..7410
                     /gene="yraP"
                     /locus_tag="QIE41_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDS3531524.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7532..8572)
                     /locus_tag="QIE41_002650"
     CDS             complement(7532..8572)
                     /locus_tag="QIE41_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDS3531525.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPLTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVIAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGIAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLA"
     gene            complement(8645..9280)
                     /locus_tag="QIE41_002651"
     CDS             complement(8645..9280)
                     /locus_tag="QIE41_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDS3531526.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9408..9926
                     /gene="yhbO"
                     /locus_tag="QIE41_002652"
     CDS             9408..9926
                     /gene="yhbO"
                     /locus_tag="QIE41_002652"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDS3531527.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9906..10349)
                     /locus_tag="QIE41_002653"
     CDS             complement(9906..10349)
                     /locus_tag="QIE41_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDS3531528.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10400..10702
                     /gene="yhbQ"
                     /locus_tag="QIE41_002654"
     CDS             10400..10702
                     /gene="yhbQ"
                     /locus_tag="QIE41_002654"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDS3531529.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(10689..11192)
                     /locus_tag="QIE41_002655"
     CDS             complement(10689..11192)
                     /locus_tag="QIE41_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS3531530.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11186..11710)
                     /locus_tag="QIE41_002656"
     CDS             complement(11186..11710)
                     /locus_tag="QIE41_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDS3531531.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11919..12914
                     /locus_tag="QIE41_002657"
     CDS             11919..12914
                     /locus_tag="QIE41_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDS3531532.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12923..13801
                     /locus_tag="QIE41_002658"
     CDS             12923..13801
                     /locus_tag="QIE41_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDS3531533.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13882..14889
                     /locus_tag="QIE41_002659"
     CDS             13882..14889
                     /locus_tag="QIE41_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDS3531534.1"
                     /translation="MTDKTIPFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15007..16251)
                     /gene="mtr"
                     /locus_tag="QIE41_002660"
     CDS             complement(15007..16251)
                     /gene="mtr"
                     /locus_tag="QIE41_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDS3531535.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(16405..18294)
                     /gene="deaD"
                     /locus_tag="QIE41_002661"
     CDS             complement(16405..18294)
                     /gene="deaD"
                     /locus_tag="QIE41_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDS3531536.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18287..18367)
                     /gene="yrbN"
                     /locus_tag="QIE41_002662"
     CDS             complement(18287..18367)
                     /gene="yrbN"
                     /locus_tag="QIE41_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDS3531537.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18474..19358)
                     /gene="nlpI"
                     /locus_tag="QIE41_002663"
     CDS             complement(18474..19358)
                     /gene="nlpI"
                     /locus_tag="QIE41_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDS3531538.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19467..21602)
                     /gene="pnp"
                     /locus_tag="QIE41_002664"
     CDS             complement(19467..21602)
                     /gene="pnp"
                     /locus_tag="QIE41_002664"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDS3531539.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21849..22118)
                     /gene="rpsO"
                     /locus_tag="QIE41_002665"
     CDS             complement(21849..22118)
                     /gene="rpsO"
                     /locus_tag="QIE41_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDS3531540.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22267..23211)
                     /gene="truB"
                     /locus_tag="QIE41_002666"
     CDS             complement(22267..23211)
                     /gene="truB"
                     /locus_tag="QIE41_002666"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDS3531541.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23211..23612)
                     /gene="rbfA"
                     /locus_tag="QIE41_002667"
     CDS             complement(23211..23612)
                     /gene="rbfA"
                     /locus_tag="QIE41_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDS3531542.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
BASE COUNT         5729 a         6444 c         6030 g         5437 t
ORIGIN      
        1 cttaacccgt attgataacc gtctggttca cggtcaggtt ggcgtgacct ggacatccac
       61 catcggtgca aatctgctgg tagtcgtgga tgatgttgtc gctaacgatg atattcaaca
      121 gaaattaatg ggtattaccg cggaaaccta cggctttggc attcgtttct ttactatcga
      181 aaaaaccatt aacgtcatcg gcaaagctgc accgcatcag aagatcttcc tgatttgccg
      241 tacgccacaa acggtacgta aattggtaga aggtggtatt gacctgaaag atgtcaacgt
      301 cggcaatatg catttctcgg aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga
      361 ccaggatctc acggacttac gttttattaa acaacgtggc gtgaatgttt tcattcagga
      421 cgtccctggc gatcaaaaag aacaaatccc tgactaaatc taaaatcgcc ttaatattgg
      481 tttgaggtaa taaaaatgca tgaaataacc ctacttcagg gattatccct ggcggcgtta
      541 gttttctttc tggggattga tttttggctg gaagccttat ttttattccg cccgataatc
      601 gtttgtaccc taactggcgc tattctcggt gatattcaga ctggcttaat taccggtggc
      661 ctgacagagt tggctttcgc cggattaacc cctgcaggtg gtgttcagcc gcccaacccg
      721 attatggcgg gtctgatgac cactgtcatt gcatggtcta cgggcgttga tgccaaaacg
      781 gcaattggtc ttggcctgcc gtttagtttg ttaatgcagt acgtcattct gttcttctat
      841 tccgctttct cattatttat gaccaaagcc gataaatgcg caaaagaggc ggatacggca
      901 gcgttttccc gacttaactg gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt
      961 gctttcctct gtacttacct ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc
     1021 gcctggctga cccacggctt tgaagtggct ggcggtattc tgcctgccgt tggttttggc
     1081 ttgctgctgc gcgtgatgtt caaagcgcaa tatatccctt acctgatcgc cggtttcctg
     1141 tttgtttgct acatccaggt cagcaacctg ttgccggttg ccgtactggg cgcaggcttt
     1201 gcggtgtatg agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc
     1261 agtaaaaatg aagaagagga ctacagcaat gggatctgaa atcagtaaaa aagatatcac
     1321 ccgtctgggc tttcgttcgt cactgctgca agcgagcttt aattacgaaa ggatgcaggc
     1381 gggcggtttt acctgggcga tgttgccgat cctgaaaaag atttataagg atgacaaacc
     1441 gggcctaagc gcagcgatga aagataacct cgaatttatt aatacccacc cgaatctggt
     1501 cggattcctg atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat
     1561 taaaggcctc aaagtggcac tgtttggccc aatcgccggg attggcgatg cgattttctg
     1621 gttcactttg ttgccgatta tggcggggat ttgctcatcg tttgccagtc agggaaacct
     1681 gctggggccg attctatttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg
     1741 gacccacgtc ggttattcag tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca
     1801 gatgattgcc cgttcggcaa ccatcctcgg gatcacggta atcggcgggc tgatcgcttc
     1861 gtatgtgcat attaacgtgg tgacatcgtt tgccatcgac agtacccaca gcgtcgcact
     1921 gcagcaggat ttcttcgata aagtcttccc gaacatttta ccgatggcct acaccctgtt
     1981 gatgtattac ttcctgcggg tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt
     2041 tgtgctctct attgtttgtt ctgcattcgg cattttgtaa atggaacgag gcactgcgtc
     2101 tggtggtgcc tctttactaa aggaatttca tcctgtgcaa acccttcaac aagttgaaaa
     2161 ctatacggcg ttaagtgaac gtgccagcga atatttattg gccgtgatcc gtagcaaacc
     2221 ggatgccgtg atttgcctgg cgaccggagc cacgccatta ctgacgtatc attatctggt
     2281 agaaaaaatc caccagcagc aggttgatgt cagccatctc accttcgtga agctcgacga
     2341 atgggtggat ctgccattaa ctatgcctgg cacctgcgaa actttcctac aacagcatat
     2401 cgtgcagccg ctggggctac gtgaagacca gctcatcagc tttcgctccg aagagataaa
     2461 tgagacagag tgcgaacggg taacgaacct gattgcgcgc aaaggcggcc tggatttatg
     2521 cgttctcgga ttggggaaaa acggtcatct tgggctgaac gaaccgggag aaaacctgca
     2581 accggcctgc catatcagtc aacttgatgc cagaacacag caacatgaga tgttaaaaac
     2641 cgcgggccgt cccgtgactc gtgggatcac cttaggcctg aaggatattc tcaatgcccg
     2701 cgaagttttg ttactggtga ctggcgaagg aaagcaggat gcgacagatc gttttctcac
     2761 ggctaaagtc tctaccgcta tcccggcttc atttttatgg ctgcataact atttcacatg
     2821 catgattgat gagatgtgtc gtcgttaact cgcctgctac aggcgttgca acgctattcc
     2881 tgctgcgacg cctgtaatca ctctccctgc tgctccagtg catacttata cagcgcattt
     2941 ttcttcacgc cgtgaatttc tgctgccagc gccgccgctt ttttcagtgg cagttctgcc
     3001 tgtagcagcg ccagcgtgcg cagggcatcg gcgggtaagt cttcttcctg tgctttatgg
     3061 ccttcgacaa tcagtaccat ttcgcctttg cgacggtttt catcttcttt gacccacgcc
     3121 agcagctcgc caacgggcgc gccgtgaatg gtttcccagg ttttggtcag ctcacgcgcc
     3181 agaacgacgt agcgggattc gcctaatacc gcaacgatat cttccaggct atctaacaga
     3241 cggtgggtag attcataaaa aatcagcgtg cgcggctccg cttcaatggc ttttagcgca
     3301 tcacggcggc cttttgattt ggcaggtaaa aagccttcat aacaaaaacg atcggatggt
     3361 aaacccgcgg cgcttaacgc agtgatagca gcacacggcc cgggtagcgg caccacgcga
     3421 atccccgctt cacggcaggt acgcaccaga tggtagccag gatcgttaat tagcggcgtt
     3481 ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca gcttcgccag cagcgtttcg
     3541 gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc gagcattaat cccaaaatgt
     3601 tgcagcaata aaccggtgtg acgcgtatcc tcggcggcaa tcagatcaac ggcctgtaat
     3661 acctccaacg cacgctgggt gatatccgcc agattgccga ttggcgtcgg tacaatgtaa
     3721 agctggccct gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg
     3781 atttaatatt gagcattgcg taaaaaaaat atcactggat acattatggt accctcaaca
     3841 ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc
     3901 ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac ggcgcaggct
     3961 gattctgcct tttacctgca acagatgcag caaagttctg atgataccag gatcaactgg
     4021 caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccggacaggc ggtggagttg
     4081 tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg
     4141 gcagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc
     4201 acacctgcag atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa aatcgatgcc
     4261 agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctc
     4321 ggcgcgaaag aaaagcagca gaatattgac gccacctggc aggcgctctc ctccatgact
     4381 caggaacagg cgaatacgct ggtgatcaat gccgacgaaa atattctgca aggctggttg
     4441 gatctgcagc gcatctggtt tgataaccgt aacgatcccg acatgatgaa agccgggatc
     4501 gccgactggc agaaacgtta tccgaacaac ccgggcgcga aaatgctgcc aacgcagttg
     4561 gttaacgtaa aagcgtttaa acccgcatcg accaacaaaa tcgccctgct gttgccactg
     4621 aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc ggcgaaaaat
     4681 atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga cgtagcagaa
     4741 caacctcagc cgcaaaccgt ggatggcgtt gccagcccgg cacaagcctc ggttagcgat
     4801 ctgaccggtg aacaacctgc ggcccagccg gtgcctgtaa gcgccccggc ggcaaccacc
     4861 gcagcggtaa gcgcacccgc gaatccatcc gcagagctga aaatctacga tacatcatca
     4921 caaccactta gccagatcct caaccaggtt caacaggatg gcgcgagtat tgtggtcggt
     4981 ccgttgctga aaaataacgt tgaagagttg ctgaagagca acaccccgct gaacgtgctg
     5041 gcactgaacc agccggagaa tatcgaaaac cgcgtcaata tttgttactt cgcgctttca
     5101 ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg
     5161 ctggtgctga tcccacgcag ttcattgggc gatcgcgtag ccaatgcgtt tgcgcaagag
     5221 tggcagaaac tgggtggcgg caccgttctg caacaaaaat ttggttccac cagcgaatta
     5281 cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg gtagcccgat tactcccaga
     5341 gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac gccaaccgat
     5401 gaccagttca ccaataatgg cggtcgtgtc gatgcggtgt acattgtggc aacgccgggt
     5461 gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag cggtgcaacg
     5521 ctgtacgcca gttcccgcag cgcacaaggc atcgcaggcc cggatttccg tctggagatg
     5581 gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctgcc gttaatgcag
     5641 caggcgctca gtgcggtgaa caacgattat tcgctggctc gcatgtatgc gatgggcgtc
     5701 gatgcctggt cgttggcaaa tcatttctca caaatgcgcc aggttcaggg ttttgaaatc
     5761 aacggtaata ccggaagtct gacggctaac ccggattgcg tgattaacag gaagttatca
     5821 tggctacagt accaacaagg tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg
     5881 atgcgtggga agcacaagcg cgtcgctggc tggaaggcaa aggactgcgg tttatcgccg
     5941 ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc cggatcaccg
     6001 tttttgtcga ggtgcgttac cgccgctctg cgctttatgg cggcgcggca gccagtgtga
     6061 cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg cgtcataatg
     6121 ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg aatgaggttg
     6181 agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat tagcgtgcaa
     6241 gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc ggcagaggcg
     6301 cttccggatg ccatctcccg tgcagccatg acgctggttc agtctctgct caatggcaac
     6361 aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt tgctgccagc
     6421 atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact aaatactgat
     6481 aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta tgcaaaacag
     6541 gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg tggcaacagc
     6601 cgcgatattg taaaagcggt agaagctgcc gttacgcgtg atatgaccat tgtggcatta
     6661 accggctatg acggcggtga acttgcaggt ttgttagggc cacaggatgt ggagatccgc
     6721 attccttcgc atcgtagtgc tcgcattcag gaaatgcata tgctgacggt aaattgcctg
     6781 tgcgatctga tcgataacac gcttttccct caccaggatg tttaaggaga atacatgaag
     6841 gcattatcgc caatcgcagt ccttctttcc gcgctgctgt tgcaaggttg tgttgccgct
     6901 gccgtagtgg gtaccgctgc tgtaggtacc aaagccgcaa ctgacccacg cagtgtcggc
     6961 acccaggtgg acgatggtac cctggaagtg cgcgtaaaca gcgcattgtc gaaagacgaa
     7021 cagattaaga aagaaacccg tattaatgtc acggcgtatc agggcaaagt gctgctggtt
     7081 gggcagtcac caaacgctga actttcggct cgcgctaaac aaattgcgat gggcgtagac
     7141 ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct gggcgaagcg
     7201 tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag cgacctggtg
     7261 aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat ggggctagtg
     7321 actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtaagcgg cgtgaagcgg
     7381 gtaaccacgg cgtttacgtt tattaaataa gccattgatc ggtcgttgaa gtaaatattc
     7441 acaccttagc cgacctgttc ccctcaccct aaccctctcc ccaaagggga gaggggaccg
     7501 tccagtgctg tattcaggag catccaacat cttaggccaa cagcgccagc ccgccgacaa
     7561 tcacacctga cactgccacc atcgcccccg tcagccataa ggctttcgcc gggaacgctt
     7621 tacgcaacat aatcagtgac ggcaaactca ccgccgggag cgtcatcaac aatgccagcg
     7681 ccggagcgat tcccatgcct gccagcatca tcgtttgtac aattggaatt tctgctgccg
     7741 ttggaatgac aaacaagcat cccgctaccg ccatcgcaat cacccacatc aggctgttat
     7801 cgacagcacc atcagcatgg gggaataacc agacgcgagc ggcacccaac acgagtacgg
     7861 caaggatata aaccgggatc gtactccaga aaagcgtcca tagcgccctg ccccagcggc
     7921 taaaaaatcc gccctgcgct tccggtatgt caatttcgac cggtgcctgc gttagcggtg
     7981 tttcacgtac ccatttttgc accagcgtcg caatcagcaa caccatcacc agccccgcga
     8041 ccagacgaat cgccgcaaac ccccagccga ggacaaaccc cataaacacc agcgtcgccg
     8101 ggtttaacaa cggattcccc atccagaatg ccagcgcccc gcccatcgat acctgttggc
     8161 gacgcattcc cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa
     8221 acagcgttcc cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc agcaaccagt
     8281 cacgcgggat cagcacctga atcaacgacc cgagcagtac gcccagcacc gccgctttcc
     8341 ataccgcgag gaaatagatc atcgcgtaat ccaacgccgc ctgccacgga ttagcatccg
     8401 cctgcgcaag gatagattta ccgatactgt gggtttcggc agccgtaaac gctttgccgt
     8461 agtaaggttg ccatttcaca taccagagac cagcaatgac aacgagaaag aaaagcgcgg
     8521 gtttccacca ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc
     8581 ccaggaataa gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga
     8641 ataattattc agctcttttg cgtaattctg aagagcttaa aatcgtcaca ccttcatgct
     8701 caggccgcat cgcctctgcc agcatgaccc gcgccacgtc tcttgcatca atggatttcc
     8761 agttacccgg taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc
     8821 tacgatcgcc cagcaacatc gaagggcgag caatggtcaa tttcggccag ttctgggcga
     8881 ttaatgcttc ttccatctcc cccttgacgc ggttatagaa aaacggcgag tgggcattgg
     8941 cacccatagc actgactacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg
     9001 tatccactac cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc
     9061 gcgtggtgcc gagacaacaa aacacaatgt cgatgggatc ggtgacctgc gccagcgcgt
     9121 cggtcagttg cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg
     9181 tcggcgcggc tatggcgtta actttcggtt cgttaatcaa catccgcagc aggtgaccgc
     9241 ccaccagccc cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt
     9301 gtctgccttg cgctccccgg ctcatgcacc aggcttaata gaccatgagg taattatccc
     9361 cgattgtggg aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa
     9421 ttgccgtttt aatcactgat gaatttgagg attcagaatt tacttcaccc gcagacgagt
     9481 tccgtaaagc cggacacgaa gtgattacca ttgaaaagca agcgggtaaa acggtgaaag
     9541 gcaaaaaagg agaagccagt gtgaccatcg ataaatccat cgatgaagtg acgccagcgg
     9601 agtttgatgc cctgctgcta ccaggcggtc attcaccgga ttatctgcgt ggcgacaacc
     9661 gttttgtcac tttcacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc
     9721 acggtccgca gttgctgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta
     9781 aaccgatcat tattgatgtt aaaaacgcgg gcgcggaatt ttacgatcag gaagtcgtcg
     9841 tcgataaaga tcagctggtt accagccgca caccggacga tctgccagcg tttaaccgcg
     9901 aggcattacg cctgctcggt gcctgagtta cgtagccaga tcattttttt accaaagccc
     9961 agcgtgttgt cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc
    10021 attctggcaa ccggaaagcg tcgattgtac gcctggcgtg cgaggtcgct ttcctcacct
    10081 tccagtctgc ggatctcacc tttaaactgc acgccgcgaa ttaacgctac cgttttcggc
    10141 tgaccgttta ccgttccagc aactgccgcc tgcggcccac tcatctgcgc gtggcgcgtt
    10201 ttttcttccg tcaaaatgta gaaggcaact ttctgcgcat caaaaagata aaaggcattg
    10261 gcgcaccaca attccccttc ctgctgcaca caccaggtga caacatgttg ttttgccaac
    10321 caacggctga tggtgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc
    10381 ttaacatgcg acgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa
    10441 taagctttat accgggatca ccacagatgt cgaacgccgc tatcagcagc accaaagcgg
    10501 caaaggggcg aaagcactgc gcgggaaagg agaactaacg ctggcgtttt ccgcgccagt
    10561 gggcgatcgt tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca
    10621 aaaagagcgt ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac
    10681 cccaaagatt aaaagcgatt gaagtgctcg tgatactcaa ccagaccggt tacgccgttc
    10741 agcgcgtcat ccgctaaacg atgtacctgg aaggcgcttt cagtgcccgg ccagcggcaa
    10801 cgcagatcgt gatgcgccgc cagctcaaag ccgaaacgac tgtacagcgc cggatcgccc
    10861 agcgtcacca ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt
    10921 tggcgtgcca gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc
    10981 cactgcaggt cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga
    11041 ccttcgtcat ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga
    11101 accagcttcg cttccgcatc actttcgaat gaacgacgca gcagggcatc aatacccggc
    11161 gcatcaatgg gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt
    11221 caggcgcggt tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta
    11281 aggctttcgg catttgctcc agttcaatgg cgtccatcag gtttttcaca tacagcccca
    11341 gctccgtatc gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt
    11401 tacgcgccgc aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg
    11461 cgttctggct aacgaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat
    11521 cacgcacatg aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg
    11581 cctgacggaa ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg
    11641 tcagttttac cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt
    11701 tatccaacac gagttttact ccctgtttca acaatcatcc tattttgcca tattagaaaa
    11761 ataacatagc ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact
    11821 ttaactgcct taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac
    11881 atttttgcgt tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg
    11941 gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc
    12001 taaaagatga tactaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc
    12061 aggaagcggt gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt
    12121 ttgcgcaccc ggacggttac gcccgttggc agcgcgccgt ggatatggcg gcgcagctgg
    12181 gtgccgacgc gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc
    12241 cgcatattga gcgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact
    12301 tttatcatcg ccattttgac gttgcacgcg tggtgctgcc gcgcgtgttg tcgattcatc
    12361 aggtgaaaca actggcgcgg gtcacgcctg taccactgga agtctttgct ttcggcagcc
    12421 tgtgcattat gtcggaaggc cgttgctatc tgtcgtcata tctgacgggt gaatcgccca
    12481 acaccgtcgg tgcatgttct ccggctcgct tcgtgcgctg gcagcaaacg ccgcaggggc
    12541 tggaatcccg cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt
    12601 atccgacgct gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag
    12661 aacccaccag tctcaatacc ctggaactgc tgccggagtt aatggcagcg aatattgctt
    12721 cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct
    12781 ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt
    12841 ggatggagac gctcgggtcg atgtccgaag gcacgcagac cacccttggc gcgtatcacc
    12901 gtaaatggca gtgagaaaag caatgaaata ttccttaggg ccagtgctgt ggtactggcc
    12961 aaaagagacg ctggaagaat tttatcagca ggccgccgcc agcagcgccg acgtgattta
    13021 tcttggtgaa gcggtatgca gcaagcgtcg ggcaaccaaa gttggcgact ggctggagat
    13081 ggcaaaatca ctcgccggaa gtggtaagca gattgtgctc tccacgctgg cgctggtgca
    13141 ggcatcctct gaactgggcg aactgaaacg ctatgttgag aacggtgagt ttctgattga
    13201 agccagcgat ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg
    13261 gcacgcgctg aactgctaca acgcggtgac actgaaaata ttgctcaaac agggcatgat
    13321 gcgctggtgt atgccggtgg agctttcccg cgactggttg gtgaatctgc ttaatcagtg
    13381 cgatgagctg ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctgcc
    13441 gctggcctac tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg
    13501 tgaaacctgc tgcattaagt atccgaacgg gcgcaacgtg ctatcgcagg aaaaccaaca
    13561 agtgtttgta ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga
    13621 gctgacatcc atgcaggggc tggtcgatgt ggtccgcctg tcaccgcagg gtactgacac
    13681 tttcgcaatg ctcgacgcct tccgcgccaa tgaaaatggc gctgcaccac tgccgttgac
    13741 ggcaaacagc gattgtaacg gctactggcg gcggctggcc gggctggagc tgcaagcgta
    13801 agtatatagc tcactttgtt aacaacttta actactcttt aatgcagtat taaagattaa
    13861 tcggtaacaa agtgagctgt tatgactgat aaaaccattc cgttttcgct actcgatctg
    13921 gcccccatcc ccgaaggttc ttcagcgcga gaagcattct cccactctct cgatctcgcc
    13981 cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgact
    14041 ggcattgcca gtgctgccac gtcggtattg attggctatc tggcggcgaa taccaccacg
    14101 ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccgttggt cattgccgaa
    14161 cagttcggca cgctcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct
    14221 ccgggtagtg accagcgaac catgatggct ctacgtcgtc atatgagcgg ggatattgat
    14281 aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg
    14341 catgtgcgcc cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc
    14401 ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt ttgcgtttgc ctcacacttc
    14461 gcgccggata tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct
    14521 cggctggaaa aaccatacgc gatggtgtgc atcaatatta tcgctgccga cagcaaccgc
    14581 gacgctgaat ttctgtttac ctcaatgcag caagcctttg tgaaactacg ccgtggcgaa
    14641 accggacaac taccgccacc gattcaaaat atggatcagt tctggtcacc gtctgagcag
    14701 tatggcgtgc agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat
    14761 ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggttaa cgggcagatt
    14821 ttcgatcacc aggcacgcct gcattcgttt gaactggcga tggatgttaa ggaagagttg
    14881 ttgggatagt gtgtcttaac gcgggaagcc ttatccgaga tggcaacgct gtcctacgta
    14941 gacctgataa gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac
    15001 cgctgattac tgatacaccg gcagcaaatt aaagctcgat aagatatgga ccagtgcgtt
    15061 gccaacgcca aataccagaa tcagcataat catcggcttg ccgccccaga cgcggaattt
    15121 cgggctacca aagcgtttac gcgatgcgcg ggccaacagc gccggaacaa ttgccgccca
    15181 gatggtagcc gctaagccag cataaccaat ggcgtacagg aatccgttag gaaacagcag
    15241 gccacccaca acaggcgggg caaaggtcag caacgctgtt ttcaaacggc ccatagccga
    15301 gtcgtcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa
    15361 cgaactcgct accgcaaagt ttgagaacac gaccagcaga agatccaggc tacggctgtt
    15421 cagcacgccg cttaatgcct gtaccagcac atcaatatta ccacccttct cggcaatgcc
    15481 aataaactca ggacgaggga tgttgcccat cgtcgccagc aaccagatgg tatacagcgc
    15541 cagtgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta
    15601 atacttcatc aggcttggca cgttaccgtg ataaccaaac gacgccagac agaacggcag
    15661 ggtcatcaac agatacggtg cataagacgc attgctttcg gcgacgttga acaatgtcgc
    15721 aggctgcaca tgccccagta ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc
    15781 cagcacaatc gccgtcatgc ggctgacggc tttagtgctc aaccacacca caaacgccac
    15841 cagcagtgca aaaccaaaac ccgctgcccg tgccgggacg ttcaatgaca tctctgcgaa
    15901 ggtatgatgc agaatcgaac cgctggcaga aatataggca taggtcagga tataaagcac
    15961 aaaggcaatg gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat
    16021 ggtgtcaaag ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga
    16081 atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaga accacgcccc
    16141 ggacatgacc acaggcagag aaaacatccc tgcgccaata atggtgccgc cgatgatcac
    16201 cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc
    16261 tctccagtga aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacacgagt
    16321 acaaaagaca gaaaaaaagc cccgatggta aaaatcgggg ctgtatatat tattttacag
    16381 attgtgttcg ctgtttagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga
    16441 gtcatcacga cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc
    16501 acgaccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gttcgccacc
    16561 gaaaccacga ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa
    16621 ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc
    16681 cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat
    16741 gtaacggctg ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac
    16801 accatcatcg cggcccactt caatgcggta cagctgcatg tcaccaacat cacgacgttc
    16861 acgacgcgga cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc
    16921 acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat
    16981 cagagtacgt tcaccctgtg ccattttcag cagtgcggcg gccagagttt cgagatccag
    17041 ctcttcacct tctgcagtcg gctgaatttt gctcagcagt gcacggtatt gatccagatc
    17101 gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacgac gtttgcccag
    17161 cagttctgcg ttcggcagtt caacttccgg aatggtcagc ttcatggtgc gttcaatgtt
    17221 acgcagcaga cgacgctcac ggttctcaac gaacagcagc gcacggccag cacggcccgc
    17281 acgaccagta cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt
    17341 aactaccagg ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag
    17401 gatgtccaga cgaccatctt tcagtcgttc cagtgtctgc tcacgcagcg cctggttcat
    17461 gtcaccgttc agcgcggcgc tattgtagcc gttacgctca agagcttctg ccacttccag
    17521 agtcgcgttt ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa
    17581 acgtaccagt gcttcgtttt tacgcatacc ccaaacagtc cagtagctct ggctgatgtc
    17641 aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg
    17701 ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc
    17761 cgggatctgc gccataatag tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc
    17821 agcttcatcc agaaccagac cgctcagttt agaaaggtcc agagtgccac gtttcaggtg
    17881 atccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa
    17941 ttgcacgtca taacgctggc cgccgtacag ggcaaccacg tttacgccgc gcatgtgttt
    18001 agagaaatcc gtcattgcct cagcaacctg taccgccagt tcgcgggtcg gtgccagcac
    18061 cagaatctgt ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc
    18121 tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg
    18181 tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc
    18241 aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta
    18301 cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa
    18361 ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc
    18421 acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc
    18481 tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg
    18541 tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg
    18601 gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg
    18661 agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt
    18721 tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc
    18781 gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg
    18841 gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc
    18901 agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcgatccc gcgattcaag
    18961 tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca
    19021 tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc
    19081 ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta
    19141 tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga
    19201 ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat
    19261 ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt
    19321 gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg
    19381 ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc
    19441 gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt
    19501 gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg
    19561 cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact
    19621 ttctcaacgc gtttgtcagc gatctgagag atgtgtacca gaccttcttt accgccgccg
    19681 atggcaacaa atgcgccaaa gtcaacgata cgggtcactt taccattgta gacgcggccc
    19741 acttcgattt ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg
    19801 tcggtcgctg cgatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct
    19861 tcggtcaggg cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg
    19921 ttgatcttga tggtatggat acgcggagcg aactcagaga tatcgccacg tggcgcgttg
    19981 atcgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagtgca
    20041 acctgcatga tctctttggt gataccttca attttgatat ccatctgcaa tgcagaaata
    20101 ccgtcgcggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa
    20161 atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca
    20221 acagcagctt tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa
    20281 gccatagaag aagaaccgtt ggattcggtg atttcagaca ccacacgtac ggtgtacggg
    20341 aatttgtcca tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca
    20401 atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag
    20461 ttgtagtgga acaggaaggt gtcagtacgt tcgcccatca gttcatcaag aacctgcgcg
    20521 tcacgtgcag tacccagcgt tgcggtaacc agcgcctgag tttcaccacg ggtaaacagc
    20581 gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg aatcatatct
    20641 ttttcacgac cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc
    20701 tcgatagcgt gcagaatttc acccagttcg ttttcgtcca gggtttcatc ttcagcaagc
    20761 agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg
    20821 gtgatgcggt aagcatcgct cagacgagct tcagccagtg cagcaacgcg cgcgttcagc
    20881 gcttcgttta ccggctccgg ctgccagtcc caacgcggtt tgccggcttc tttcaccagt
    20941 tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc
    21001 atctggtctt cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta
    21061 cccgcaacaa ccagatccag tttgctctct ttcagctcgt cctgagtcgg gttcagtacg
    21121 tactggtcat tgatgtaacc tacgcgggca gcaccaatcg gaccattgaa cggaatacca
    21181 gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacctgcggg
    21241 ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc
    21301 gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct
    21361 tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta
    21421 acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca
    21481 aataccgcgg tgtcatccat gctaaccata acagcagcag tagcctgacg agccatcatg
    21541 ccggtttcca gagtcacggt gtgttgaccg tactggaatt tacgaacgat cggattaagc
    21601 aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat
    21661 cttctgcgca tcctcgcgac taatgacaac cctaacccag cccaatgtgg gtaaagcctc
    21721 tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc
    21781 cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact
    21841 cgcaagaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct
    21901 ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga
    21961 cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca
    22021 gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca
    22081 gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag
    22141 aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa
    22201 caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg
    22261 ttaacattac gccgggtatt caaccaccag gcgacgaggc gcgacgcggc cttcatcgtc
    22321 aatttcgccc ataccgataa acttgccgtt ctcaccttct gtaacgcgaa ccagtccttc
    22381 cagcggcgca ccggatgtac gaaccgggtt accatttttg aagtaaacag aagacgttaa
    22441 cggaagattc accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc
    22501 aagtaactcc gcggctggaa tatcctgctg ttcggcttgt tcaaccagtt cacgcagatg
    22561 ttccagggtc accatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat
    22621 aacatgcgcg ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt
    22681 gccttttgag cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa
    22741 ttcataaacg gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc
    22801 gtacagtttt ttgccctggt atttcagtgc tgaatacatc gatgggatct gttcgatatc
    22861 gccacggaaa gtatccagtg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc
    22921 ttcaacgatt tgtccatcgg cgtcagacgt atcggtacgc tgtccaagac gcgcaatcac
    22981 ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca
    23041 aatcggcaac atgccggtcg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc
    23101 gttataaatc cgtttcactt tttgcagcgc atcgttgctg gacataccct gaggtttatc
    23161 cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc
    23221 ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg
    23281 tttgacatac gcatcccttc aaccagcgag ttgtcgtaga agaaggtcag ttccggcacg
    23341 atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcctgcaac
    23401 gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt tacatatact
    23461 ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccaga
    23521 cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct
    23581 acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggcc
//
LOCUS       DAOTRK010000072        23221 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.072, whole genome shotgun sequence.
ACCESSION   DAOTRK010000072 DAOTRK010000000
VERSION     DAOTRK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.072"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            202..1545
                     /locus_tag="QIE41_002668"
     CDS             202..1545
                     /locus_tag="QIE41_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDS3531543.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHSGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1770..3425
                     /gene="opgD"
                     /locus_tag="QIE41_002669"
                     /gene_synonym="mdoD"
     CDS             1770..3425
                     /gene="opgD"
                     /locus_tag="QIE41_002669"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDS3531544.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3565..3789
                     /locus_tag="QIE41_002670"
     CDS             3565..3789
                     /locus_tag="QIE41_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDS3531545.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3852..4391
                     /gene="rimL"
                     /locus_tag="QIE41_002671"
     CDS             3852..4391
                     /gene="rimL"
                     /locus_tag="QIE41_002671"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDS3531546.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNVQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(4383..5363)
                     /locus_tag="QIE41_002672"
     CDS             complement(4383..5363)
                     /locus_tag="QIE41_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDS3531547.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQDAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            5487..6479
                     /gene="tehA"
                     /locus_tag="QIE41_002673"
     CDS             5487..6479
                     /gene="tehA"
                     /locus_tag="QIE41_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDS3531548.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6476..7069
                     /gene="tehB"
                     /locus_tag="QIE41_002674"
     CDS             6476..7069
                     /gene="tehB"
                     /locus_tag="QIE41_002674"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDS3531549.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSVANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            7372..8040
                     /locus_tag="QIE41_002675"
     CDS             7372..8040
                     /locus_tag="QIE41_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDS3531550.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPHSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(8075..9286)
                     /gene="ydcO"
                     /locus_tag="QIE41_002676"
     CDS             complement(8075..9286)
                     /gene="ydcO"
                     /locus_tag="QIE41_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDS3531551.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FAFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMSALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            9339..9875
                     /gene="sutR"
                     /locus_tag="QIE41_002677"
     CDS             9339..9875
                     /gene="sutR"
                     /locus_tag="QIE41_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDS3531552.1"
                     /translation="MENLARFLSTTLKQLRQQHGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGKGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            9948..11909
                     /gene="rlhA"
                     /locus_tag="QIE41_002678"
     CDS             9948..11909
                     /gene="rlhA"
                     /locus_tag="QIE41_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDS3531553.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDFYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVSEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLAGY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(12001..12231)
                     /locus_tag="QIE41_002679"
     CDS             complement(12001..12231)
                     /locus_tag="QIE41_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDS3531554.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQQSRKPEAGQR"
     gene            12453..12629
                     /gene="hicA"
                     /locus_tag="QIE41_002680"
     CDS             12453..12629
                     /gene="hicA"
                     /locus_tag="QIE41_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDS3531555.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIK
                     EPLRKAILKQLGLS"
     gene            12675..13091
                     /gene="hicB"
                     /locus_tag="QIE41_002681"
     CDS             12675..13091
                     /gene="hicB"
                     /locus_tag="QIE41_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDS3531556.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            13170..14576
                     /locus_tag="QIE41_002682"
     CDS             13170..14576
                     /locus_tag="QIE41_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDS3531557.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPAPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            14821..15966
                     /locus_tag="QIE41_002683"
     CDS             14821..15966
                     /locus_tag="QIE41_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDS3531558.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATLFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            15984..16997
                     /locus_tag="QIE41_002684"
     CDS             15984..16997
                     /locus_tag="QIE41_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3531559.1"
                     /translation="MTYAVEFGNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQTNGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            16998..17939
                     /locus_tag="QIE41_002685"
     CDS             16998..17939
                     /locus_tag="QIE41_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3531560.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            17929..18723
                     /locus_tag="QIE41_002686"
     CDS             17929..18723
                     /locus_tag="QIE41_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3531561.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            18745..20169
                     /gene="patD"
                     /locus_tag="QIE41_002687"
     CDS             18745..20169
                     /gene="patD"
                     /locus_tag="QIE41_002687"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDS3531562.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(20266..20361)
                     /gene="yncL"
                     /locus_tag="QIE41_002688"
     CDS             complement(20266..20361)
                     /gene="yncL"
                     /locus_tag="QIE41_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDS3531563.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            20556..20729
                     /gene="ortT"
                     /locus_tag="QIE41_002689"
     CDS             20556..20729
                     /gene="ortT"
                     /locus_tag="QIE41_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDS3531564.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            20815..21048
                     /locus_tag="QIE41_002690"
     CDS             20815..21048
                     /locus_tag="QIE41_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDS3531565.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(21049..21498)
                     /locus_tag="QIE41_002691"
     CDS             complement(21049..21498)
                     /locus_tag="QIE41_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDS3531566.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAA
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(21495..22013)
                     /gene="mddA"
                     /locus_tag="QIE41_002692"
     CDS             complement(21495..22013)
                     /gene="mddA"
                     /locus_tag="QIE41_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDS3531567.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTLAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            22194..>23221
                     /gene="curA"
                     /locus_tag="QIE41_002693"
     CDS             22194..>23221
                     /gene="curA"
                     /locus_tag="QIE41_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDS3531568.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVQGFDVCLDHHADDFAEQLAKACPKG
                     VDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAG"
BASE COUNT         5795 a         5608 c         6037 g         5781 t
ORIGIN      
        1 cgaagcggtg tcgctgacaa gtgaagttgt ttgattatga taacttgatt gcaaaatatt
       61 attaacaatt aaagcaatta tgttacagca aaatggataa tattgatgtt ttcgcggcga
      121 gatcacagtt tgtaaattct tcccgccgga gtgaatgcgg ttacctacac tccagattac
      181 tgaccactgg aggcagacac tatggcgaac agcatcacgg cggatgagat tcgggaacag
      241 ttttcgcagg caatgtcagc catgtaccag caagaagttc cgcagtacgg cacgctgctg
      301 gaactggtag ctgatgtgaa tctggctgtg ctggaaaaca atcctcaact gcacgaaaaa
      361 atggtaaatg cagacgagct ggcgcgactg aatgttgaac gtcatggggc gattcgtgtt
      421 gggactgcac aagagcttgc tactcttcgg cggatgtttg ccattatggg gatgtacccg
      481 gtgagctatt acgatctctc gcaggcgggg gtgccggtac attcgacagc atttcggccc
      541 attgatgatg cttctctggc gcgtaatccc ttccgcgttt ttacttcatt actccgcctt
      601 gagcttatcg agaacgaaat tttgcgccag aaagcggcgg agattctgcg tcagcgcgat
      661 atcttcaccc cacgttgtcg acaactgtta gaggaatatg agcagcgggg cggttttaac
      721 gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa cgtttcgttg gcaccagtca
      781 gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg aacatcggtt aattgctgat
      841 gtggtctgtt ttcctggatg ccatatcaac cacctgacgc cacgtacgct ggatattgac
      901 cgggtgcagt cgatgatgcc tgaatgcggg attgaaccca aaattctgat cgaagggccg
      961 ccgcgccgcg aggtaccgat tttactacgc cagaccagct ttaaagcact ggaagagacg
     1021 gtgttgtttg cagggcagaa acagggtacg cataccgcgc gctttggtga aatagagcag
     1081 cgtggtgtgg cattaacgcc gaaagggcga caactgtatg atgatcttct gcgtaacgct
     1141 ggaaccgggc aggataatct cactcaccaa atgcatttac aggaaacctt ccgcactttt
     1201 cctgacagtg agtttttaat gcgtcagcaa gggttggcat ggttccggta ccgtctaacg
     1261 ccttcaggtg aggcgcatcg tcaggcgatt cattccggag acgatccaca gcccttaatt
     1321 gaacgtggtt gggtagtggc gcaacccatt acctatgaag atttcttgcc tgttagcgcg
     1381 gcggggatct tccagtcaaa tctgggtaat gaaacgcagg cacgcagtca cggtaatgcc
     1441 agtcgcgaag catttgagca ggcgttgggc tgcccggttt tggatgagtt ccagctttat
     1501 caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc tttaaactct gaccatccgc
     1561 ctttgcaaaa atttgcctga ttttacaaac gaatcaggct catcccatcg acataaaaaa
     1621 attgccgatt tatgcatatt ctctcagttc aacaattgga ttattaataa atattgtcta
     1681 gagtgagcgg tcataaataa gcactttctt gccgctgaaa acgaccagcg cgggaccatt
     1741 cacaacacca gaaggactca ctttcaggta tggatcgtag acgatttatt aaaggttcaa
     1801 tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct caggcggcat
     1861 tcgcggcaga ttctgatatt gccgacgggc aaacccaacg ttttgacttc tccattctac
     1921 agtcaatggc gcacgactta gcgcaaacag cgtggcgagg tgcgccgcgt ccgttacctg
     1981 acactctggc gacaatgacg ccgcaggctt ataacagtat tcaatacgac gccgaaaaat
     2041 cgctctggca taacgttgag aaccgtcaac tggacgctca gttcttccat atgggaatgg
     2101 gattccgtcg ccgcgttcgt atgttttctg tagatcccgc aacacatctg gcgcgtgaaa
     2161 ttcactttcg cccggagttg ttcaaataca acgatgcggg tgttgatacc aaacaattag
     2221 aagggcaaag cgatctcggt tttgccggtt ttcgcgtgtt taaagccccc gaactggcgc
     2281 gccgtgatgt cgtatcattc ctcggtgcga gttatttccg cgccgttgac gacacatatc
     2341 aatacggtct atcggctcgc ggcctggcga tcgacactta caccgacagt aaagaagagt
     2401 tccccgactt taccgccttc tggtttgata cggtaaaacc gggggcaacc acctttaccg
     2461 tttatgcgtt gctcgatagc gccagcatta ctggtgccta taagttcact atccattgtg
     2521 agaaaagtca ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa gacattaaac
     2581 agctgggcat tgcgccgatg accagcatgt tcagctgcgg tactaatgaa cgtcggatgt
     2641 gcgacaccat tcatccgcaa atccatgact ctgatcgtct gtccatgtgg cggggcaacg
     2701 gcgagtggat ttgccgtccg ctgaacaatc cgcaaaaatt gcagttcaat gcttacaccg
     2761 acaacaaccc gaaagggttt ggtttattgc aactggatcg tgatttctct cattatcagg
     2821 acattatggg ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt aacaagtggg
     2881 gtaagggcac catcggcctg atggaaatcc caactacggg tgaaacgctg gataacattg
     2941 tctgcttctg gcagccagaa aaagctgtaa aagcgggtga tgagtttgca ttccagtatc
     3001 gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt atggcgacgc
     3061 gtaccggcat gggcggtttc ccggaaggtt gggcgccagg tgaacactat cccgaaaaat
     3121 gggcgcgtcg ttttgccgtc gatttcgttg gtggtgatct gaaagctgcc gcaccaaaag
     3181 gcattgagcc ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa attctctata
     3241 ttgaacccat tgatggttat cgtattcagt ttgactggta tccgacttcg gactccactg
     3301 atccggtcga tatgcggatg tatctgcgtt gtcagggcga cgctatcagt gaaacatggc
     3361 tgtatcagta tttcccgcca gcgccggata aacgtcagta tgttgacgac cgcgtgatga
     3421 gttaatcgtt ttttcttcgg caccttcttc gggaggtgcc gtctggttaa acacgatccc
     3481 gctcgcattt ttccctaagt taaatgagta atctgatagt gtgtatttca gatacacctt
     3541 gtcagccact aacagggagt gcgtatgttt ccagaatacc gagatttaat atcccgtctg
     3601 aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac acaataaact tgatcatgaa
     3661 attgccagaa aggaaggttc cgacggtcga gggtacaatg cggaagtggt ccgcatgaaa
     3721 aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc tgcagcagga gagcgtcaaa
     3781 gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt ttaacaactg catggattga
     3841 ctggagataa gatgaccgaa acgataaaag taagcgaatc acttgaatta catgctgttg
     3901 cagaaaatca cgtcacacct ctttatcagt taatctgtaa aaataaaacc tggttacagc
     3961 agtcgctaaa ctggccgcag tttgttcaaa gtgaagagga cacgcgaaaa acggtgcagg
     4021 gcaatgtaat gttgcatcaa cgcggatatg ccaaaatgtt catgattttc aaagaagatg
     4081 aacttatcgg cgttatctcg tttaatcgta ttgaaccact gaataaaacc gctgaaatcg
     4141 gctactggct ggacgaatct catcaggggc aggggatcat ttctcaggcg ctgcaggcat
     4201 tgattcatca ttacgcccag tctggtgaac ttagacgctt cgtgatcaaa tgtcgggtgg
     4261 acaatgtgca aagcaaccag gtcgccttgc gcaatggttt tatccttgaa ggttgtctga
     4321 aacaggctga gttcctgaat gatgcctatg atgatgtgaa cttatacgcg cgtattatcg
     4381 attcacaata accctgccag cggcgttcgc gtaatgcgct cctcgccgtt aatgactttt
     4441 ggaccacgga catgaaccgt gtcgccatca aaggcttcaa cgacggcgtg gtcggttaac
     4501 tccacatgtt tttcaattat caccgcacct gtaatacggc tctcaccttg tattacaacg
     4561 tgctcgtcga gcagaatcgg tccgccacgt accacggcat ttccgccaat caatacatga
     4621 tgttttaaca cacaattacc ttccacaatg gcatattccg ccacctgcga actgtaatga
     4681 atcgtcggaa tggcatcttc ttctatgcca gctttcacct gcgcgtgacc gtagacttta
     4741 gcgcaatcgc atagccagac gttgttctct tcattacctt caacgctggc aaaatcaaaa
     4801 acttcggcgc gatgttcaat aaaagcatac cggacgacgg catcgccata aatttgtgcc
     4861 tggtgaacaa tacgcgaggc gctaaccctt gcgcgatcat aaatttgtag caggagttga
     4921 tggtcgggcg ttaagccttg tgcggcgacg atcatagaat gttgatcaat taaggcgtga
     4981 ccaaatattc tacattgacc acaaactaat gagtcacgaa tggtgacgct gtcacttata
     5041 tatgcatcct ggctgatttc actattatcg atccagacgt tatccgttgc gtaaacctct
     5101 ccccaaagaa cgctggtgcc ggtgatacgc gtattaccgg aaatcaccgt tccgccgaac
     5161 gcaatagcat tctgatcgta aatccagcaa tttccttcct gtgctaacac cgtttcgcga
     5221 tcgatccagc cgccagcggt tcctgctatc acatcgttaa aatcgctaat ggcgataatc
     5281 tgccgtaata acacactttt tttagtgcca tcttcctgat aactaaaggc tcgctgttct
     5341 tcacttagac gatatttgcg catagcgttt tcccacaggt gacttactat aaccgtagca
     5401 aattctgcgg ctctggctat gctcgagaaa attccataaa atgcatttca aatgtacttt
     5461 ataaattaaa caaaatgagt aagaagatgc agagcgataa agtgctcaat ttgccggcag
     5521 gctactttgg tattgtgttg gggacgatag ggatgggatt tgcctggcgc tatgccagcc
     5581 aggtttggca ggtcagccac tggttagggg atgggctagt gattctggcg atgatcatct
     5641 ggggattatt gactagcgca tttattaccc gactcatacg ctttccgcat agcgtgctgg
     5701 cggaagttcg ccatccagtg ctgagcagtt ttgtgagttt gtttccggca acgacgatgc
     5761 tggtggcgat tggttttgtt ccgtggtttc gtccactggc ggtgtgcctg ttcagttttg
     5821 gtgtcgtggt gcaactggct tatgccgcat ggcaaactgc gggattatgg cgcggatctc
     5881 accctgaaga agctaccacg cctggactgt atctgccgac agttgccaac aactttatca
     5941 gcgcaatggc ctgtggtgcg ttgggctaca ccgacgcagg tctggtgttt ttaggcgcag
     6001 gcgttttctc atggctaagc cttgaacctg tgatcttgca gcgtctgcgc agttcgggag
     6061 aattacccac ggcactgcgg acctcactcg gcattcagct cgctcctgca ctggtggcct
     6121 gtagtgcctg gctgagcgtc aacggcggcg agggtgacac gctggcgaaa atgcttttcg
     6181 gttatggact gctgcaactg ctgtttatgc tacgtctgat gccatggtat ctctcccagc
     6241 catttaatgc ttcattctgg agtttctcgt tcggcgtatc tgcactggca accaccggtt
     6301 tgcatctggg gagtggcagc gataatggat ttttccatac gctggcggtg ccgctgttta
     6361 tctttaccaa ttttattatt gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg
     6421 gaaaattgtt agtcagaacc gagcgcgccg ttttaatgaa agcagaggac aaagaatgat
     6481 cattcgtgat gaaaactatt ttactgataa atatgaatta acccgcacac actctgaagt
     6541 gctggaagcg gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct gtggcaatgg
     6601 tcgtaacagt ctttacctgg cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc
     6661 catgagtgtc gccaacgtcg agcgcattaa gtccattgaa aatctggata atttacacac
     6721 ccgggtcgtt gatctgaata acctcacatt tgatggacag tacgatttta ttctttcgac
     6781 tgtggtgctg atgttccttg aggctaaaac catcccaggg ctgattgcca atatgcaacg
     6841 ttgcactaaa cctggcggtt acaacctgat tgtggcggcg atggataccg ctgattatcc
     6901 atgtaccgtc ggcttcccgt ttgccttcaa agagggggaa ttacgtcgat attacgaagg
     6961 ctgggagatg gtgaaataca atgaagacgt cggcgagctg caccgcaccg acgccaacgg
     7021 taatcgtatt aaactgcgtt tcgccacgat gctggcacgt aaaaaatgat ccggtaagaa
     7081 caaagcgcgt gaaaattccc cacgctgaga tggtttactg ttcttctttt cggtaagcat
     7141 attttttatc gaagggatgt gaaattaatc acagtagtcg aagtttttta gccgcttaac
     7201 ttactgaaat ttaactactg atgattgact tagccccttt ttcgacattg actatgtcgt
     7261 ctgaaaaggg gctaaaaaat ttattttcac caacactttt tttgccacaa cacgaagcgg
     7321 cgctttttgc tataacttag aaagtaatat aatcatctca ggaaactatt catgcgtacc
     7381 acatcatttg cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg ttgtgcctct
     7441 aaaatcaccc agccagataa atattctggt tttttaaaca attactctga tttaaaagaa
     7501 acaacctcgg caacaggtaa acctgtttta cgttgggtag acccgagttt tgatcaaagc
     7561 aaatatgaca gcatcgtctg gaacccaatc acttattatc cggtaccgaa accttctacc
     7621 caggtagggc agaaagttct ggataaaatt ttgaactata ccaacaccga aatgaaagaa
     7681 gcgatagcgc agcgtaaacc actggttacc accgctgggc cgcatagtct gattttccgt
     7741 ggggccatta ccggtgtaga taccagcaaa gaagggctgc aattctatga agtggttcct
     7801 gttgcattag tggttgcggg gacgcaaatg gctacaggcc accgtaccat ggatactcgc
     7861 ctctattttg aaggtgagct gattgatgca gcgactaata aaccggttat caaagtcgtt
     7921 cgtcagggcg aaggtaaaga cctgaataac gaaagtacgc caatggcttt cgaaaatatt
     7981 aaacaagtta ttgatgacat ggcgaccgat gccaccatgt ttgacgttaa caaaaagtag
     8041 ttccagacgc gccatcgtta gatggcgctt tttattatcg gtttctgtta gcgattaaat
     8101 tcaacaccac gtaacaaacg cctccggcaa ttaatcccca aaacgcagaa ccaatcccga
     8161 acagcgtcaa tccacttgcc gttaccagaa atgccaccac cgccgcgtct cgctcacgct
     8221 cattatgcag cgcctgatac aaactgccgc caatggtact caacagcgcc agacctgcca
     8281 gcatctggat ccaacttacg ggcagggcag acatcatccc ggtaatggca ctaccaaaca
     8341 gacctgcgag caaatagaaa atgcctgcaa cggcagcggc cagccaacgt tgatctttat
     8401 ccggatgcgc ttccgggctt tggcaaatag ccgcggtgat tgccgcaata ccgacggaat
     8461 aaacgccgaa aggggaaaaa aacagtgcca gcaatccagt aaatacaatt aatggcgaaa
     8521 caggagccga atatccggct gctttcattg ctgcgatacc cggtgcgttt tgcgatgcca
     8581 tcgtcaccag aaaaagggga agtgcaacgc tcagactgtg agcaaacgca aaatcagggg
     8641 tgatataagt ggggagaacg ggtttaaaga caacatcagt tgtgacaacg tcaccctgcg
     8701 cgataacgat gacgatccca ataatcatcg cggcaattac cgcatagcgc ggcgcaacgg
     8761 ccctggttgc cagccatacc agcaacatac tgccacacaa cgtaaattga ccatccaggc
     8821 tcgcaaacgc ctgtaaacca aagcgtaata aaatcccggc aagcattgcc gccgcaagcg
     8881 agtgcggaat aatgcgcatc agacgagcaa agagtcccgt tatgccgcag aggactatta
     8941 acgcgttggt gacaataaaa acgccgatgg cttcgttaag tgttagtccc tgcaatccgg
     9001 tgaccaacaa agccgcgcca ggcgttgacc atgcagtgag aacaggtacg cgataccata
     9061 atgtcagagt cagcgtactg acgcccattg ccagccccag cgccgtcatc cagccagaga
     9121 tttgtgcagt ggtggctccg gcgacaatcg ctgcttgcca gattattgcc gctgaactgg
     9181 cgtagccaat taataccgcc agaaaccccg caagtagcgt gggcggagga atagaaaaca
     9241 gacgcatagt cacctcgtgc gttataacgt acaggtaccg tagcatttgt ccgttataac
     9301 gcacaagtga taaacttccg ttttgccgga ggagtcgcat ggaaaatctc gctcgctttt
     9361 tatccaccac acttaaacaa ctacgtcagc agcatggctg gagtctttcg cgactggcag
     9421 aagcgacggg cgtttctaaa gcgatgttag ggcagattga gcgtaatgag tccagcccga
     9481 cggtcgcgac attatggaaa attgccaccg ggttgaatgt gccattttcg acatttattt
     9541 ctccgccgca gtccgctacg ccttcagttt atgatccgca acagcaggcg atggtgatca
     9601 cttcgctgtt tccttatgat ccacaactct gctttgaaca cttctcgatt cagatggcac
     9661 ccggtgcaat aagcgaatcg acgccccatg agaagggagt aatcgaacat gttgtcgtca
     9721 ttgatgggca acttgatctg tgcgttgatg gtgaatggca gagccttaat tgtggaaaag
     9781 gcgctcgatt tgctgcagac gttacgcatg tctatcgtaa tggtggggag caaatcgtac
     9841 attttcattc cctcatccat tacccgcgca gttaagcggg aaaactattt cgcaacgtcg
     9901 cgcttgtggc taaaatagcc gccatttttc agctactgga taagaatgtg accgtatctt
     9961 ctcatcgact tgaactgtta agcccggcac gcgatgccgc cattgcccgc gaagctattt
    10021 tgcacggtgc cgatgctgtt tatatcggcg gccctggttt tggtgcccgt cataatgcca
    10081 gtaatagcct gaaagatatt gccgaactgg tgccgtttgc ccatcgttat ggtgcaaaaa
    10141 ttttcgtcac gcttaacacc attttgcatg atgatgagct ggaacctgcg caacggctga
    10201 ttaccgactt ctaccagacc ggcgtcgatg cgctgattgt tcaggatatg gggattctgg
    10261 aacttgatat tccgccgatt gaactgcacg ccagtacgca gtgcgatatt cgcacagtag
    10321 aaaaagcgaa gttcctctct gatgttggct tcacgcagat tgtgctggcg cgagagctga
    10381 atcttgagca gatccgcgcg attcaccagg ccacggacgc gaccattgaa ttctttattc
    10441 atggcgcact gtgcgtggcc tattcgggtc agtgctacat ttctcatgcg caaacagggc
    10501 gtagcgccaa ccgtggcgat tgctcgcagg cgtgccgttt gccatacaca ttgaaagacg
    10561 atcaggggcg ggtggtttcc tatgaaaaac atctgctgtc gatgaaagat aacgatcaga
    10621 ctgccaacct cggcgcgctg attgatgctg gcgtacgctc cttcaagatt gaagggcgtt
    10681 acaaagatat gagctacgtg aagaatatca ccgcccatta tcgccagatg ctggatgcca
    10741 ttattgaaga acgtggcgat ctgacgcgtg cttcatcagg tcgtactgaa catttctttg
    10801 ttccatcgac ggaaaagact ttccaccgtg gtagcacaga ttattttgtg aatgcccgta
    10861 aaggcgatat tggcgcgttc gattcgccga aatttatcgg cctgccggtg ggtgaagtat
    10921 tgaaagtggc gaaagatcat ctcgatgttg ccgtttccga accactggca aatggcgacg
    10981 gcctgaacgt gatgattaaa cgtgaagtcg tcggctttcg tgccaatacg gtcgagaaaa
    11041 ccggagaaaa tcagtaccgc gtctggccca atgaaatgcc agcagagttg cacaaaattc
    11101 gcccgcatca cccactaaac cgtaatcttg atcataactg gcagcaggca ctgacaaaaa
    11161 cctccagcga acgtcgggtg gcggtagaca ttgaactggg cggctggcag gaacaactga
    11221 ttctgaccct caccagtgaa gagggtgtca gcatcacgca tacgctggac gggcagttcg
    11281 acgaagccaa taacgccgaa aaagcaatga acaatctaaa ggatggtctg gcaaaactgg
    11341 ggcaaaccat ctattacgcc cgcgatgtgc aaattaattt gccgggtgcg ctgtttgtac
    11401 caaacagtct gttaaatcag ttccgccgtg aagccgccga catgctggat gctgcacgtc
    11461 ttgccggtta ccagcggggc agccgtaaac cggttgctga tcctgcgccg gtttatccgc
    11521 aaacgcatct gagtttcctc gcgaacgtat acaaccagaa agcgcgtgaa ttttatcatc
    11581 gctacggtgt gcagctgatt gacgcggcgt atgaggcaca tgaagagaag ggcgaagtcc
    11641 cggtgatgat cactaagcat tgtctgcgct ttgccttcaa tctgtgcccg aaacaggcga
    11701 aaggcaatat caaaagctgg aaggcgacgc caatgcaact ggttaacggc gatgaagtat
    11761 taacgctaaa gtttgattgc cgcccatgcg agatgcacgt catcggcaaa attaaaaatc
    11821 acattctgaa aatgccgtta ccgggaagtg ttgtcgcctc cgtaagtccg gatgagctgc
    11881 tgaaaacatt gccgaagcga aaagggtaaa atgccagttt tctggttact cacaacttat
    11941 tgaatctgca tgatattgtt ggccgggtaa ggcgtcacgc cgcatccggc aataaatgac
    12001 tcaacgttgt cccgcttctg gtttgcgcga ttgttgccag taaaaatgct cgcgcaaacc
    12061 ttccgccgac tcttccgcca cagcacgcaa ttcatcgctg tccgcttcat gacgcagctg
    12121 atgatccaca ttctttaccc acacaaattc atgtcctttg tgccctgcca tgagttgtcc
    12181 tgagaacagc gcacacgtta ataagacaac cgataacgcc ttcgtaaaca tcctgccacc
    12241 tttttcttac cttttgccgc tatgatgccg atcgtttctt gaggttatta ttcagttttg
    12301 caaattagcg caaagaaatt atggaatctt ccttcctgat tttgcattgc attctgccgt
    12361 tgcggcgatt tagtgctatt ttcgagcaaa ttacacacgg aggtaaacac taatgtttac
    12421 tttgttgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc agacgttggc
    12481 tcgaatctca gggcgtcgat gtagcgaatg gcagcaacca tttaaaactc aggtttcacg
    12541 ggaggcgcag tgtcatgccg cgtcacccca gcgaagagat taaagaacca ttgcgtaaag
    12601 ccattctgaa acaactcggt ttgagttaat cgccaattaa aaaggttaat gacatgcgag
    12661 agacagtcga aattatgcgt tatcccgtca ctcttacacc cgcgccggaa ggcggttata
    12721 tggtttcttt tgtggatatc cctgaagcgt tgacccaggg cgaaactgtc gctgaagcga
    12781 tggaagcggc aaaagatgct ttactgaccg catttgattt ttattttgaa gataacgagc
    12841 ttatcccttt accttcgcca tcaaatagtc acgatcactt tattgaagtg cctttgagcg
    12901 tcgcctctaa agtattgctg ttaaatgctt ttttacagtc agaaatcact cagcaagagt
    12961 tagccaggcg aattggcaaa cctaaacagg aaattactcg cttatttaac ttgcaccatg
    13021 cgacaaaaat cgacgccgtc cagcttgcgg ctaaggcgct tggcaaagag ttatcgctgg
    13081 tgatggttta attacagtta acgaaaagtt gtcattttta acaactgata tagactgccg
    13141 aatcatctgc acataattac gattcgataa tgaaaaaata ccagcagctt gcagaacaat
    13201 tacgcgagca gattgcgtcg ggtatctggc aacctggcga tcgtttgcct tcgttgcgtg
    13261 accaggtggc gctttctggc atgagcttta tgaccgtgag ccatgcctat cagttgctcg
    13321 aaagtcaggg atatattatc gcgcgaccgc agtcgggtta ttacgttgcg ccacaggcaa
    13381 taaaaatgcc gaaagcgcca gtcattcctg tcactcgaga tgaagcagtc gatatcaaca
    13441 cctatatttt tgatatgttg caggccagtc gcgatccgtc ggtagttccg tttgcctcgg
    13501 cctttcccga cccgcgactt ttccccctcc aacaactcaa ccgctcgctg gcgcaggtaa
    13561 gcaaaaccgc cacggcgatg agcgtgattg aaaacttacc gccaggaaac gcagaactgc
    13621 gtcaggctat tgctcgtcgc tatgccttac agggcatcac tatttctcct gatgaaattg
    13681 tcattaccgc cggggcgtta gaggcattaa acctcagttt acaagcggtc actgaaccgg
    13741 gcgattgggt gatagtagag aatccttgtt tctacggcgc gttgcaggcg ctggagcggt
    13801 tacggctgaa ggcgttatcg gtggcgacgg acgttaaaga agggattgat ctcaaggcgc
    13861 tggaactggc gttgcaggag tatccggtga aagcgtgctg gctgatgact aatagccaga
    13921 atccactcgg atttacctta acgccgcaga aaaaagcgcg gctggtagcg ttgctcaatc
    13981 agtacaacgt aacgctgatt gaagatgacg tgtacagcga actttatttt ggacgggaaa
    14041 aaccgctgcc tgcgaaagcg tgggatcgcc acgatggcgt tttgcattgc tcttcgtttt
    14101 cgaaatgtct ggtgcctggt tttcgtattg gttgggtcgc cgccggaaaa catgcacgta
    14161 aaattcaacg cttgcagttg atgagtacgc tttccaccag ctcaccgatg cagcttgcac
    14221 tggtggatta cctttccacg cgccgatacg acgcccatct tcgtcgcttg cgtcgccagc
    14281 ttgcggagcg taaacaacgt gcctggcagg cactgctgcg ttatctgcct gcggaagtga
    14341 aaattcatca taatgacagc ggttattttc tctggctgga gctccccgcg ccgttagatg
    14401 cgggcgaatt aagcctggca gcactgacgc atcatatcag tattgcgccg ggtaaaatgt
    14461 tttctaccgg tgaaaactgg tcacgttttt tccgttttaa taccgcgtgg cagtggggcg
    14521 agcgcgaaga acaggcggtt aaacaattag gcaaacttat tcaagaacga ctgtaatagc
    14581 gtttaattta attcctctca ggttaagtaa tatgaatttc gaatagcagt catatttcct
    14641 aactccttgt ctatactcca gaagataacc ttacagacgg cataatgcgc ggtagctcac
    14701 aacctgatta aattttctca ggggcgaagg tgtgcctgct agccgccgtc tatggttaaa
    14761 caaggagata tttttacggc acggcggctg aacaattaat tacgacagga gtaagacttt
    14821 atgagcaaga catttgcccg cagcagcctg tgtgcgctca ccatgacaat aatgaccgct
    14881 cacgccgccg aaccacctac caatttagat aaaccggaag ggcgactgga tattatcgcc
    14941 tggccgggat acatcgaacg cggacaaact gataaacaat acgactgggt aacgcaattc
    15001 gaaaaagaga ctggctgcgc ggtgaatgtg aaaaccgccg cgacttccga tgaaatggtc
    15061 agtctgatga ccaaaggggg ttacgatctg gttacggcat ccggcgatgc ctcgctgcgc
    15121 ctgattatgg gcaaacgcgt gcagccgatt aataccgcat tgattcccaa ctggaaaacg
    15181 ctcgatccgc gcgtggttaa aggcgactgg tttaacgtta gtggcaaagt ttatggcaca
    15241 ccttaccaat ggggtccgaa cctgttgatg tacaacacta aaaccttccc gacgccgccg
    15301 gatagctggc aagtggtttt tgttgagcag aatctgccgg acggcaagag caataaaggc
    15361 cgcgttcagg cttatgatgg ccctatctac attgcggacg ctgcgttgtt cgttaaagcc
    15421 actcagccgc agttgggcat cagcgatccg tatcaactca ccgaagaaca gtaccaggcg
    15481 gtgctgaaag tgctgcgcga tcaacatagt ttgatccatc gctactggca tgacactacc
    15541 gtgcaaatga gcgatttcaa aaacgagggt gtggttgctt ccagtgcatg gccctatcag
    15601 gccaacgccc tgaaagccga aggccagcct gtcgcgaccc tattcccgaa agagggcgtt
    15661 accggttggg ctgacaccac catgctacat agtgaagcga aacatccggt ttgcgcctac
    15721 aaatggatga actggtcatt aacgccaaaa gtgcagggcg atgtggcggc ctggtttggc
    15781 tcgttaccgg tagtgccgga agggtgtaaa gccagtccgt tattaggcga gaaagggtgt
    15841 gaaacaaacg gttttaacta tttcgataaa atcgccttct ggaaaacacc tatagcagaa
    15901 gggggcaagt ttgttcccta cagtcgctgg acgcaggatt acattgccat tatgggcggt
    15961 cgctaacttc gctggggtgt tttatgacgt acgcagtgga gtttggcaac gtctcgcggt
    16021 tgtacggtga cgtgcgcgca gtagatggcg tcagtattgc gataaaagat ggtgagttct
    16081 tctctatgct ggggccgtcc ggctccggca aaaccacctg cctgcgcctg attgctggct
    16141 tcgaacagct ttccggcggg gctatctcta tctttggtaa acccgccagc aatctgccac
    16201 cgtgggagcg ggacgtgaat actgtcttcc aggactacgc actatttccg catatgtcga
    16261 ttcttgacaa cgtcgcctat ggtctgatgg tcaaaggcgt gaataaaaaa cagcggcatg
    16321 cgatggcgca agaggcgctg gagaaagtgg cattggggtt tgtacagcaa cgcaaaccgt
    16381 cacaactttc tggtggtcag cgccagcgag tcgccatcgc cagagcactg gtgaacgaac
    16441 cgcgtgtgtt attgctggat gaaccgctcg gcgcactgga tctcaaattg cgtgagcaga
    16501 tgcagctgga actgaaaaaa ctgcaacagt ctctcggtat cacttttatc ttcgttaccc
    16561 acgatcaggg cgaagcgtta tcgatgtccg atcgtgtggc ggttttcaat aatggacgca
    16621 ttgagcaggt cgattccccg cgcgatctct atatgcgccc gcgcacgccg tttgttgccg
    16681 ggttcgttgg tacatcgaat gtttttgatg gactgatggc agagaaactt tgtggcatga
    16741 cgggaagctt cgccctgcga ccggaacata tccgcctcaa cacccctggt gaactgcaga
    16801 ccaatggcac gatccaggcg gtgcaatatc agggcgcggc aacacgtttt gaactgaaat
    16861 tgaacggcgg tgaaaaactg cttgtgagtc aggccaatat gacaggcgaa gaactgcctg
    16921 ccacgctcac gcccggacaa caggtgatgg tttcctggtc gcgtgatgtg atggtgccgc
    16981 tggttgagga gaggtgaatg gcgatgaatg tattgcaatc accttcacgt ccaggtctgg
    17041 gtaaggtgtc cggtttcttc tggcgtaatc cggggctggg gctgttttta ctgctgcttg
    17101 gcccgctaat gtggtttggc attgtctatt tcggctcgct gctgacactg ttatggcagg
    17161 gattttatac ctttgacgat ttcaccatgt cggtaacgcc ggaactgacg ctggcgaata
    17221 tccgtgcgct gtttaatccg gcgaattacg acatcattct ccgcacgctg actatggctg
    17281 tggcagtcac tatcgccagc gccattctgg cttttccaat ggcgtggtat atggcgcgct
    17341 ataccagcgg gaaaatgaaa gcgttttttt atattgcggt aatgttgccg atgtgggcga
    17401 gctacattgt taaagcctat gcctggacgt tattgctggc aaaagatggc gtggctcagt
    17461 ggtttttaca acatcttggg ctggaaccac tgctgactgc gttccttaca ttacctgcgg
    17521 tgggcggcaa tacgctgtca acttccgggc tggggcgctt tctggtgttt ctctatatct
    17581 ggctgccgtt catgatcctg cccgtgcagg cggcgcttga gcgtttgccg ccgtcattgt
    17641 tgcaggcgtc ggctgatctt ggcgcgcgtc cacggcaaac ctttcgctat gtggtgctgc
    17701 cgctggcaat ccctggtatt gccgctggct ctatctttac cttttcactc acactgggcg
    17761 attttattgt tccgcagctg gttggccctc caggatattt tatcggcaat atggtttatt
    17821 cccagcaggg agcgattggc aatatgccga tggcggcggc attcaccctg gtgccgattg
    17881 ttctcatcgc actgtacctg gcgttcgtga aacgtctggg agcgttcgat gcactctgaa
    17941 cgcgcaccgt ttttcctcaa actggcggcc tggggcggcg tggttttcct acattttccc
    18001 atcctgatta tcgccgccta tgcgtttaac actgaagatg cggcgtttag ttttccaccg
    18061 cagggcctga cgctgcgctg gtttagcgtg gcagcacagc gtagtgatat tcttgatgcc
    18121 gtgacactgt cacttaaagt ggcggcgctg gcgacattaa tggcgctggt gttagggacg
    18181 ctggccgctg ccgcgctgtg gcgacgagac tttttcggca aaaacgccat ttcgctgtta
    18241 ctgctgctgc ccattgcgct gccgggcatt gtcactggtc tggcgttgtt aaccgccttt
    18301 aaaaccatca atctggagcc gggatttttc accatcgtgg tcggtcatgc gactttttgt
    18361 gtagttgtgg tgtttaacaa tgtcatcgcc cgttttcgcc gcacctcgtg gagtctggtt
    18421 gaggcgtcaa tggatcttgg ggccaatggc tggcaaacct tccgctacgt agtgttgccg
    18481 aatctcagtt cggcgttact ggcaggagga atgctggcgt ttgccttgtc gttcgatgaa
    18541 atcatcgtga cgacctttac ggcaggtcat gaacgaacgt taccgttgtg gttgctcaat
    18601 cagcttgggc gaccgcgtga tgtaccggta accaacgtgg tggcactgct ggttatgttg
    18661 gtaacaacct tgccgatcct gggggcctgg tggctaaccc gcgaaggcga caatggtcaa
    18721 taaccactga tacaggaata tgctatgcaa cataagttac tgattaacgg agaactggtt
    18781 agcggcgaag gggaaaaaca gcctgtctat aatccggcaa cgggggacgt tttactggag
    18841 attgccgagg catccgcaga gcaggtcgat gctgctgtgc gcgcggcaga tgcagcattt
    18901 gccgaatggg ggcaaaccac gccgaaagtg cgtgcggaat gtctgctgaa actggctgat
    18961 gttatcgaag aaaatggtca ggtttttgcc gaactggagt cccgtaattg tggcaaaccg
    19021 ctgcatagtg cgttcaatga tgaaatcccg gcgattgtcg atgtttttcg ctttttcgct
    19081 ggtgcggcgc gctgtctgaa tggtctggcg gcaggtgaat atcttgaagg tcatacttcg
    19141 atgatccgtc gcgatccgtt gggggtcgtg gcttcgatcg caccgtggaa ttatccgctg
    19201 atgatggccg cgtggaaact ggctccggcg ctggcggcag ggaactgtgt agtgcttaaa
    19261 ccatcagaaa ttaccccgct gaccgcgttg aagttggcag agctggcgaa agatatcttc
    19321 ccggcaggcg tgattaacgt actgtttggc agaggcaaaa cggtgggaga tccgctgacc
    19381 ggacatccca aagtacggat ggtgtcgctg acgggctcta tcgccactgg cgagcacatc
    19441 atcagccata ccgcgccgtc cataaagcgt actcatatgg aacttggagg caaagcgcca
    19501 gtgatagttt ttgatgatgc ggatattgaa gcggtggtcg aaggtgtacg tacttttggc
    19561 tattacaatg ccggacagga ttgtactgcg gcttgtcgga tctacgcgca aaaaggtatt
    19621 tacgatacgc tggtggaaaa actcggtgct gcagtggcaa cgttaaaatc gggtgcaccg
    19681 gatgacgagt ctacggagct tggaccttta agctcgctgg cgcatctcga acgcgtcagc
    19741 aaggcggtag aagaggcgaa agcgacaggg cacatcaaag tgatcaccgg tggtgaaaag
    19801 cgcaagggta atggctatta ctatgcaccg acgttgctgg ctggcgcatt acaggacgat
    19861 gccatcgtgc aaaaagaggt atttggtccg gtagtgagtg ttacgctctt cgacaacgaa
    19921 gaacaggtgg tgaactgggc gaatgacagc cagtacggac ttgcatcctc ggtatggacg
    19981 aaagatgtgg gcagggcgca tcgcgtcagc gcgcggctgc aatatggttg tacctgggtc
    20041 aatacccatt tcatgctggt aagtgaaatg ccgcacggtg ggcagaaact ttctggttac
    20101 ggcaaggata tgtcacttta tgggctggag gattacaccg tcgtccgcca cgtcatggtt
    20161 aaacattaag gataatattg cagatcgtaa gagtatgaga agatcttgcg tactgtcaga
    20221 gcactgattt ctctgacagt acgtgctgcg tgcaaaatca ctacatcaaa taaaccagcc
    20281 aaatctcata gagataagag taaacaaacc aacagcagca aagacattta tcagtactac
    20341 agttctactg gaaacattca tttttgccac cttttatcat tgcccctatg tgtatagtac
    20401 gggttaagaa aatccgcaat aatactcatt ctgaagatgg ataagggcaa gttgctgttt
    20461 gatgattttt tctcaaactg gtgataaaac ttgatgcacg tcaaaaaatg acgcatattt
    20521 gcgcgcgttt tattcatctg gctggacgcc cgtacatgtc tctctatcaa cacatgcttg
    20581 ttttttatgc ggttatggca gcaatcgcat ttcttatcac ctggtttctt tctcacgata
    20641 agaaacgcat ccgtttctta agcgcctttc tggtgggggc aacatggccg atgagtttcc
    20701 cggtggcgct gttgttttca ctgttttaaa tcttactcac tggcttgacg gcaccacgca
    20761 gacttatatc atttggatga atcgataaat ttcacaagtg gctaaggaga aagtatgtcg
    20821 catctggatg aagtcatcgc gcgcgtggat gccgcgattg aagagagcgt cattgcccat
    20881 atgaacgaat tattaatcgc cctgagcgat gacgcggagt taagtcggga agatcgctac
    20941 acccagcagc agcgtctgcg cacagcgatc gcccatcacg gtcgcaagca taaagaagat
    21001 atggaagcgc gccacgaaca gttaaccaaa ggtggcacca tcctctgatt aaaatgtgcg
    21061 cctggccacc agccaggcac caaccacaag taaaattgcg ccacaaattg ggccgatcaa
    21121 cgcccgtagt ggtacgccat ggctgcggaa gatatccagc accagaccgc caatcagctg
    21181 gctggcgacc agtgccgcaa tagttgtcgc cgctcccaca ttctgatatc cgctgatact
    21241 ggcgaagaca aaaaacgatc ccagcaagcc aggtataagt gtccaccagc gcacgctgga
    21301 caccagttcg ccaaaccccg ccatgccttg tttaaaccat aaaatagaaa cgaacagcac
    21361 aattcctacc aaagagttca gcaacatggc gatgagaatg gtagaggagg tctgggtgat
    21421 gcgcaccatc agcgtgttct ggactaccag accaattccg gcggcaatca aaaaagcaag
    21481 ggtaagcgac tgattcatcc aatcgcgtcc ggttcagtgc gctcgtcgag ttgcaactgc
    21541 ataaatgtca gatccagcca gcgcccaaat ttagtgccta cctgcggcat ttgtgcggtg
    21601 acgacaaatc ccaacgactg gtgcagatgc aaagacgcct gattttgcga ttcgatcccg
    21661 gcgaccatga catgcttccc gcaatcccgc gcttcatcaa tcaatcggct taacaattta
    21721 cgcccaagac ctttgccctg atgatcggga tggacataaa ccgaatgttc cacggtatgg
    21781 cgaaaaccat cgaaactacg ccagtcgcca aacgaggcat atcctgtaac gacgccatct
    21841 tcttcactca ccagcaccgg ataacctgct aaagtccgcg cttcgaacca ggcaatgcgg
    21901 ttatctgcat ccaccgtttg gtcattccag atagccgccg tatacaacac ggcgtggtta
    21961 taaatttccg caatggcagc acagtcggct ttgcgggcaa aacggatgga catgtctggc
    22021 ctcgataaaa taatgatgat gatgttatca atactatcga aaacagcccg aagcaacgga
    22081 ttcctcgatg aaattattgc gtgttgcaga ggggatgtga cggctgcaaa caaaaccaat
    22141 cccgtgagcc tgctactatt gatggattca tgccaaaagc acaggagagc attatggggc
    22201 aacaaaagca gcgtaaccgc cgttgggttc tggcctcgcg tccgcatggc gcaccggttc
    22261 cggagaattt ccgtcttgaa gaagatgatg tcgccacacc gggtgaagga caggtgttac
    22321 tgcgcacagt ttatttgtcc ctggacccgt atatgcgtgg acgtatgagc gatgagccat
    22381 cttattcacc gcctgttgat attggcggcg tgatggtcgg cggcacggtg agccgtgtcg
    22441 tggagtcgaa tcatcctgat tatcagcctg gcgactgggt gctgggctac agtggatggc
    22501 aggactatga catatccagt ggtgatgatt tggtgaaact tggcgatcat ccgcaaaatc
    22561 catcgtggtc gctgggtgtg ctggggatgc caggctttac tgcttatatg ggcctactgg
    22621 atatcggcca gcctaaagag ggcgaaacgc tggtggtagc tgcggcgaca ggaccagtag
    22681 gggcgacggt gggtcaaatc ggcaaactta aaggttgtag agtggttggg gtggccggtg
    22741 gcgcggaaaa atgccgccat gctactgagg tgcagggctt cgatgtttgt cttgaccatc
    22801 acgcggatga ttttgccgaa caactggcga aagcgtgccc aaaaggcgtt gatatctatt
    22861 atgaaaacgt gggcggtaaa gtatttgatg cggtgctacc gttacttaat acatctgcgc
    22921 gcattcccgt ctgcggctta gtgagcagct ataacgctac agagctacca cccggcccgg
    22981 atcgtttacc tctgctgatg gctacggtgc tgaaaaaacg tattcgcttg caaggattta
    23041 ttatcgctca ggattatggt caccgcatcc atgagtttca gaaggagatg gggcaatggg
    23101 tgaaagagga taaaatccac taccgcgaag aaattactga cggtttagag aatgcgccac
    23161 agacatttat cggcctgctg aagggtaaaa acttcggcaa agtggtgatc cgcgtggcgg
    23221 g
//
LOCUS       DAOTRK010000073        22797 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.073, whole genome shotgun sequence.
ACCESSION   DAOTRK010000073 DAOTRK010000000
VERSION     DAOTRK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.073"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            35..940
                     /gene="yijE"
                     /locus_tag="QIE41_002694"
     CDS             35..940
                     /gene="yijE"
                     /locus_tag="QIE41_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDS3531569.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSALLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSAVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKNI"
     gene            complement(967..1584)
                     /locus_tag="QIE41_002695"
     CDS             complement(967..1584)
                     /locus_tag="QIE41_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDS3531570.1"
                     /translation="MKASLALVSLLTAFTSNSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(1915..3018)
                     /gene="gldA"
                     /locus_tag="QIE41_002696"
     CDS             complement(1915..3018)
                     /gene="gldA"
                     /locus_tag="QIE41_002696"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDS3531571.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(3029..3691)
                     /gene="fsa"
                     /locus_tag="QIE41_002697"
     CDS             complement(3029..3691)
                     /gene="fsa"
                     /locus_tag="QIE41_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDS3531572.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDTIPGIVVKIPVTSEGLAAIKILKKE
                     GITILGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(3703..6204)
                     /gene="ptsP"
                     /locus_tag="QIE41_002698"
     CDS             complement(3703..6204)
                     /gene="ptsP"
                     /locus_tag="QIE41_002698"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDS3531573.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPRIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSEGLSCAEAIVAS
                     AHHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            6513..7592
                     /locus_tag="QIE41_002699"
     CDS             6513..7592
                     /locus_tag="QIE41_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDS3531574.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            7607..7927
                     /locus_tag="QIE41_002700"
     CDS             7607..7927
                     /locus_tag="QIE41_002700"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDS3531575.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            7978..10275
                     /locus_tag="QIE41_002701"
     CDS             7978..10275
                     /locus_tag="QIE41_002701"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDS3531576.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAHILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASRKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            10241..11119
                     /locus_tag="QIE41_002702"
     CDS             10241..11119
                     /locus_tag="QIE41_002702"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDS3531577.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            11121..11462
                     /locus_tag="QIE41_002703"
     CDS             11121..11462
                     /locus_tag="QIE41_002703"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDS3531578.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11449..12300)
                     /locus_tag="QIE41_002704"
     CDS             complement(11449..12300)
                     /locus_tag="QIE41_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3531579.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(12449..14182)
                     /locus_tag="QIE41_002705"
     CDS             complement(12449..14182)
                     /locus_tag="QIE41_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDS3531580.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(14365..17016)
                     /gene="ppc"
                     /locus_tag="QIE41_002706"
     CDS             complement(14365..17016)
                     /gene="ppc"
                     /locus_tag="QIE41_002706"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDS3531581.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(17318..18469)
                     /gene="argE"
                     /locus_tag="QIE41_002707"
     CDS             complement(17318..18469)
                     /gene="argE"
                     /locus_tag="QIE41_002707"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDS3531582.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     NLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            18623..19627
                     /gene="argC"
                     /locus_tag="QIE41_002708"
     CDS             18623..19627
                     /gene="argC"
                     /locus_tag="QIE41_002708"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDS3531583.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLNLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            19635..20411
                     /gene="argB"
                     /locus_tag="QIE41_002709"
     CDS             19635..20411
                     /gene="argB"
                     /locus_tag="QIE41_002709"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDS3531584.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            20472..21845
                     /gene="argH"
                     /locus_tag="QIE41_002710"
     CDS             20472..21845
                     /gene="argH"
                     /locus_tag="QIE41_002710"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDS3531585.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVENAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(21895..22587)
                     /gene="araD"
                     /locus_tag="QIE41_002711"
     CDS             complement(21895..22587)
                     /gene="araD"
                     /locus_tag="QIE41_002711"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDS3531586.1"
                     /translation="MDTLKEKVLAANLSLPLHQLVTFTWGNVSGIEREKGVMVIKPSG
                     VEYSQMGIADMVVVDLQSGEVIDGHRKPSSDTDTHLALYRAFPDIGGIVHTHSRHATI
                     WAQAGKDLTAFGTTHADYFYGIIPCTRPMKDDEINGQYEYETGRVIIETFRQREISPA
                     DIPAVLVNSHGPFAWGTDPANAVHNAVVLEEIAYMNLFTQQLHPHLTAIQQPLLDKHF
                     LRKHGANAYYGQ"
BASE COUNT         5166 a         5762 c         6347 g         5522 t
ORIGIN      
        1 agtgtccaca ggaaaactac acactggatc tctcatgtct gccgcaggaa agagcaaccc
       61 actggcaatc agtggcctgg ttgtgctcac acttatctgg agttatagct ggattttcat
      121 gaagcaagtc accagctaca tcggtgcctt cgacttcacc gccttacgct gcattttcgg
      181 cgctctcgtt ttattcattg tccttttatt acgtggtcgc ggaatgcgcc cgacaccgtt
      241 taaatacacc ttagccattg ccctgttaca aacctgtggg atggttggcc tggcgcagtg
      301 ggcgctggtt agcggaggag cggggaaggt ggcgatcctg agctatacca tgccgttctg
      361 ggtggtgatt ttcgccgcgt tgtttctcgg tgaacgcctg cgacgtgggc aatatttcgc
      421 gatactgatt gccgctttcg gcttattttt ggtgttgcag ccgtggcaac tcgatttctc
      481 ttcgatgaaa agtgccctgc tggcaatcct ctccggtgtt agttgggggg cgagcgcgat
      541 tgtcgcgaaa cgcctatacg cccgccatcc acgcgtggat ttattgtcgt taacatcctg
      601 gcagatgctt tatgcggcgc tggtgatgag tgcggtcgct ttactggtgc cacaacgtga
      661 aattgactgg cagcccaccg tgttctgggc tctggcctac agtgcgattc tggcgacggc
      721 gctggcgtgg agcttatggt tgtttgtatt gaaaaacttg cctgccagta ttgccagctt
      781 aagcacactg gccgttcccg tttgcggcgt actcttttcc tggtggttgc tcggtgagaa
      841 tccgggggcc gttgaaggta gcggtattgt gctgattgtg ctggcactgg cgctggtgag
      901 ccgtaagaaa aaagaagctg tcagtgtaaa aaacatctga attttttctt catgtggggc
      961 gatcgcttat ttaacaaaat accgatagtg ccccaccatc cgccagctaa acagcacgtc
     1021 ttcttcctgc gcgcctgcgc caatgttatg tatcaccagc ggcgtaccgt cgcgggcaaa
     1081 gccatctgac accaccccaa tatgcgccag cccgttatcc agtcgccagg agacaatatc
     1141 gcccgcttga tagtcactgg ggttcttgct ggtggggcgt gttttatcat ggcggctaaa
     1201 ccaggtttcc agattaggca cccgacggtg atcgatgttg ctgtccgggc gctttaactg
     1261 ccatttttgc gggtactcag caaaattctt cgccatatct tcgtgaacca gtttctgcaa
     1321 atcgaccttc tggctgcgca aggcgcggat caccacatcg gaacatacgc cgcgttcttg
     1381 cgggacatca ccgccaggat aggtaagctg cacatacgcc ggatcgtaaa atagcgtgct
     1441 gccgatttgc tgtctggcac cgtctgcgat ggcaaggttg gtgttggcct ggatttgtac
     1501 cacggttggt ggaacggccg gagattttaa ggagttgctg gtaaacgccg ttagcaggct
     1561 gaccagcgcc agtgaagctt tcatctggtt cgtccgtgaa aataagagtc acagattcga
     1621 ccttccgggc taacgattgt tctgtagaga aaatgtccag aagcggataa aaaattcctg
     1681 aacaacgtgt gagtttcaga cgggtgataa cgtataaaaa gtgctcgaac agtcccctct
     1741 cccctttggg gagagggtta gggtgagggg aacaggccag ctccggggcg aatatttacc
     1801 ctcatcccga ccctctccct gaaagggcga gggggaaaaa catgcccgca gtgacgagtg
     1861 agccactttg cctgcactct caaactcccg gacaatccgg gagtttggag taggttattc
     1921 ccactcttgc aggaaacgct gaccgtactg gtcggctacc agcagtgcag cgtaaacctg
     1981 atctggcgtc gcgccgccag gcatgttgtg gatggtttca ccttctgcac atgccgcttc
     2041 tgccacaatt cgcattttcg ccgggacatc ttctttaata tccagttgag cgagagttat
     2101 tggcaaacct accgcatggc taagtgcagc tacggtttcg atttcctcca ccggcgcatt
     2161 ttccagaacc agctgcgtca gcgtaccaaa tgccactttt tcaccgtggt aatagtgatg
     2221 cgcgtccggg atagcggtca ggccgttatg caccgcgtga gccgccgcca gcccgccgct
     2281 ttcaaaacca acgccgctca aataggtgtt cgcttcaatc acgcgctcca gcgccggagt
     2341 cactacatgc tgttcggcgg caagcatcgc tttttcgcct tcttccagca gagtgttgta
     2401 gcacagttct gccagtgcca gcgccgcctg ggtacacttg ccgcccgcca tagtggtcgc
     2461 gccgctacgg gaacaggcgc gtgcttcaaa ccaggttgcc agcgcatcgc cgatacccgc
     2521 cgctaacaga cgtgcaggtg cgccagcgac gattttggtg tcgacaatga ccatattcgg
     2581 gttatttggc aacatcagat agcggtcaaa ttcaccatca tcggtgtaga taacagacaa
     2641 tgcgctgcac ggtgcgtcgg tagaggcgat agtcggtgcg atcgctaccg gaacacccat
     2701 gaaatgtgcc agtgctttgg cagtatcgag agtttttccg ccaccgatac cgagaattgc
     2761 gccacactgc gcagtctccg cgatgccacg cagacggtcg atctcatttt gcgaacattc
     2821 accgccaaac ggcgcaattt ctactaccag tccagcatct ttaaagcttt tctcgacagt
     2881 ggattgagca aaacctaaaa caaatttgtc acccaccact aaccagcgtt ctgccagcgg
     2941 cttcaggtat tcgcccagac gattaatcac atcagcgccc tggatgtatt tacccggtga
     3001 ttgaataatg cggtccataa ttgctccttt agagatgagt agtgccaaat gcggcattcc
     3061 agtcgtgttc gaacttctct atagctgact ctaccgcagg ggtgttgagc atttgttgcg
     3121 ctacatctaa gggcagggtg atggattcac atcctgccag taaacagtcc agcgcctgac
     3181 gcggcgtttt aaagctggcc gccagcacca tgctttctgg tgcgtgcatt tctaacagcg
     3241 cttgcagctc ctgaaccgta cgaatgccgt ctccgccctg ggcatctacg cggttaacat
     3301 acggagcaac gtattttgcc cctgccagtg cggctaataa cccttgtgcg gcgctatata
     3361 cagcggtgcc aagtatagta ataccctctt ttttcagtat tttaattgct gccagacctt
     3421 cggaagtcac cgggatttta accacaatgc ccggaatggt gtcgcgcagg cgcttcgctt
     3481 cttccaccat tccctgcgca tcacggctca tggtctgagc aaacagaatg ccctcatcac
     3541 cgatcgcttt ttgcaggcgc ggcagcactt cccagatgga ctccttgctg gcagcgataa
     3601 tgctcgggtt agttgtcacc ccggcaatcg ggaatatacg tgccagacgt tcgacttctg
     3661 cgacgttagc ggtgtccaga tacagttcca tgatgtttcc ttttacagtt ccagttcatg
     3721 ttgcagcagg ctggcgatag cgtcggcaga ggcggcgtta accagcgcgt tacggaattc
     3781 ttcgtgcatg atgcggcgag cgaggcgcga gaaaatgcgc atgtgctgat cgcccgcagc
     3841 atgtttgttc agggttaaca taatgatgaa ttgtgcttca tcatcgcccc agcgcaccgg
     3901 cgcttgcaga cgcgctacgc tgatggtgga ttgctcaata tgttccgatt tgctgtgggg
     3961 aatggcaaaa ctaaagccca gaccggtaga gaaaacggcc tcgcgtgccc acaagtcggc
     4021 ttccagttta cgcggatagc ggcagcggcc cgccagtagc aggttatcgg tcatgccttt
     4081 cagcacttct tctttgctgc gccagtcgct ttccagcgtg atgcactcgg cggtgaccag
     4141 cggcgcgtcc tgttgggtca tgcggaattg cgccagcagg tgttccactt ccagcgaagt
     4201 acggcaggcc attgcctggt tgagcaactg gcggcattcg cggctatcaa gttgcgccat
     4261 ccgcgctttc gccgccggaa ttgatggtgc gctcatgctc agctcatcca gccctaaacc
     4321 gaccagcaac ggcagcacgg aacctttcgc tcccagttca ccgcacagac caatccattt
     4381 gccctggcga tgcaccgcct gcacggcgta atcgagcgcc cgtaagaatg ccggattcag
     4441 gctgttgtag tgacgagtaa ccttagcgtt atcgcgatct actgccagca gatattgcgt
     4501 caggtcatta ctaccaatac taaagaaatc aatctcttca cagcattgat cgatgatgaa
     4561 catcaccgac ggcacttcaa gcatgatgcc aagctggatc ttctcatcaa acggaatatg
     4621 ttcattgcgc agttgctgtt tggcttccgc cagtttttct ttcacccata agatctcttc
     4681 cattgaagag atcatcggga tcatgatttt caggctgccg tgagcggagg cacggagaat
     4741 cgaccgtagt tgtgtggtaa ataacgacgc gtactcttca taaatacgca cggcgcgata
     4801 accgaggaac gggtttgcct ctgcgggaat gttcagataa tcaacgggtt tgtcaccgcc
     4861 aatatccata gtgcgcacaa taatgctgcg tccgttggcg gactccagcg cctgacaaaa
     4921 aatgttgtac agctcgcttt cgcccggtgc gctggtgcga tccatataga gcatttcagt
     4981 gcggaacaaa ccaactcctt ccgcaccatt gccgaatgcg gcctgcgctt ccacggagtg
     5041 agcgatgtta gcagcaattt caatgcggat accatcagcg gtacgggctt gttgggtcag
     5101 ccagacacgc tgttgctcac gcagggcatc ctgtacgcgg gcttcttgct gataataacg
     5161 ggctacggct tcccctggct caaccacaat cgctccggcg ttgccgtcga tataaatcgt
     5221 ttgatgttgc cacggggtaa gggcatcaat atccacacca accagcgtcg gaatgttgaa
     5281 cgaacgggca agaattacgg tatgtgaggt ggtgccgccg cttttgagca acaatccttt
     5341 gaggtgattt ttatccagtt cgaggaactg gctgggggtt agttcatcag ccatacaaat
     5401 ggcgggctgc gtcagtttgc ccggtgccgg gaagcgttgc tcaccgtaga tttgctggag
     5461 taactggaag cagacgtcgc gtacgtccag ggcgcgttct tgcaggtagc tgctgctgga
     5521 acgggcgaac tcttcgcaga agtggtgtgc actggcgaca atcgcttcgg cacagcttaa
     5581 tccctcgctg acgcctgcca gtaaatgttc gcgcagggaa gtatcgccag ccagggatcg
     5641 gtgcgcttcc agaatcgcgc tggtagcacc gtcgctatcc agcagacgaa actctatgtt
     5701 tttcagtacc agcgtcaggc cgttttccag tgcggattgt tcggcatcaa cgcctttggc
     5761 agcgggaaga ttacccagcg cattgagatc taaagaagag atcggcgtca gaatgccgcc
     5821 tgcgctaccg ctgcacacgg tgcgggcgcg gataattcgc ggattcagat tggtcagtga
     5881 aaccggcagt ggttccagtt cgtcagattt tacttccgcc agcggtgcgt cgcagtgggg
     5941 gaattcatcg cgcagccatt ggcttaaacg ctggtgcgct tcctgttcgt cggccccgga
     6001 aatcagtaac tggcagttat cgcccaccag cgtatcggtg ccaatcagcg caagggcgct
     6061 tttggcgttg cccttgcggt cagtgcgcag gttatgccac tcaatttgtg atgaaaaagt
     6121 attacacagc gtttcaacgt ggcttgccgg gcgcgcatgt acgccgttag gtagctcaca
     6181 aataaattcc acaatcaggg ccattgcctc tctcctggta cggttttctg tttacaggat
     6241 aagagcgcac ggcaacggcc tgccatgtga caaatctgcc aaaagctgga caaatgtaat
     6301 gtaaccgtca atttgcgacg cgtctcacaa gacgccgttt tgcggcatgc ttccgggttg
     6361 ccgcaagtta tgaggcggat cgcatttttg tactgatatt acaaaagtcc agtaaatggc
     6421 ctttttatcc actgtttgct ccgcctgcga ttgcctattg tttcctccat caaaaatatg
     6481 gacatgggct tttgcaagcc cgggagcagc ttatgaatga gttggtgcag atcctgaaaa
     6541 atacccgtca gcacttaatg acgggcgttt cacacatgat tcccttcgtg gtatcgggcg
     6601 gtattttgct ggcggtttcc gtcatgttgt atggcaaagg cgcagtgccg gatgccgtag
     6661 ccgatccgaa tctgaaaaaa ctgtttgata tcggcgttgc aggtttgacg ctgatggtgc
     6721 ctttcctcgc tgcttacatt ggttattcca ttgcagaacg ctctgcgctg gccccgtgcg
     6781 ctatcggtgc ctgggtcggc aacagctttg gtgcgggctt ctttggtgcg ctgatcgccg
     6841 ggattattgg cggcatcgtg gtgcattacc tgaagaaaat tccggtgcat aaagttctgc
     6901 gctcggtgat gccaatcttc atcattccga tcgtcggcac actgattacc gcaggcatca
     6961 tgatgtgggg gctgggcgag cctgtggggg cattgaccaa cagcctgact cagtggcttc
     7021 aggggatgca gcagggtagc attgttatgc tggcggtgat catgggtctg atgctggcgt
     7081 tcgatatggg cggtccggtt aacaaagtgg cctatgcctt catgctgatt tgcgttgctc
     7141 agggtgtata taccgtggtg gccattgctg ccgtgggtat ttgtatcccg ccgctgggga
     7201 tggggctggc gacgctgatt ggtcgtaaaa atttctccgc agaagagcgc gaaaccggca
     7261 aggcggcgtt ggtgatgggg tgcgttgggg ttactgaagg ggcgattcct ttcgccgctg
     7321 ccgatccgct gcgtgttatt ccttccatca tggtcggttc agtttgtggt gcagtaactg
     7381 cggcgctggt cggtgcgcag tgctatgcag gctggggcgg tctgattgtg ctgccggtgg
     7441 ttgaaggcaa gctgggttat atcgcagcag tagctgtcgg agcagtggtg acggctgttt
     7501 gtgtgaacgt gctgaaaagt ctggcgcgta aaaatgggtc ttcgactgat gaaaaagaag
     7561 acgacctgga tttggatttt gaaattaatt aattgaggaa cgagccatga cgaaaattat
     7621 tgcagtaacc gcgtgtccat ctggtgttgc ccatacctat atggcggcag aggcgctgga
     7681 aagtgccgcg aaagcgaaag gctgggaagt gaaagtagaa acccagggct cgattggtct
     7741 ggaaaatgaa ctgactgcgg aagacgtggc gagcgccgat atggtgattc tgaccaaaga
     7801 tatcggcatc aagtttgaag agcgttttgc tggcaagacc atcgtgcgcg tcaacatcag
     7861 cgacgcggta aaacgcgctg atgccattat gagcaaaatt gaagctcacc tggcacaaac
     7921 cgcttaagtt cttatcccgc ccgtaagggc gggttcgctt tcccacagga gttcctcatg
     7981 acgaatcgta tctctcgcct caaaactgca ctgtttgcca atacccgtga aatctcgctg
     8041 gagcgggcgc tgctttatac cgccagccat cggcaaaccg aaggcgaacc ggtgatattg
     8101 cgccgggcga aagcaacagc gcatatcctt gaacatgttg aaatttcgat tcgtgatgaa
     8161 gaactgattg ccggtaaccg tacggtgaaa ccgcgcgccg gaattatgtc gccggaaatg
     8221 gacccttact ggctgctgaa agagctggat caattcccga cgcgtccgca ggaccgcttt
     8281 gctatcagcg aagaagataa acgtatctac cgcgaagagt tgttcccgta ctgggaaaaa
     8341 cgttcgatga aagatttcat taacgggcag atgacggatg aagtaaaagc cgcgaccagc
     8401 acgcagattt tcagcatcaa ccagacggat aaaggccagg ggcacattat tattgattac
     8461 ccgcgcctgc tgaatcacgg gctgggtgag ctggtggcac agatgcagca acattgtcag
     8521 caacagccgg agaatcactt ttatcaggca gcgctgttac tgctggaagc ctcgcggaaa
     8581 cacattctgc gttacgctga actggcggaa acgatggcgg caaactgcac agatgcccag
     8641 cgtcgcgaag aactgctgac tattgcggag atctcccgcc ataacgccga acataagccg
     8701 cagacgttct ggcaggcgtg ccagttattc tggtacatga acatcattct gcaatacgaa
     8761 tccaacgcca gttctctgtc tttagggcgc ttcgaccagt atatgttgcc gttctatcag
     8821 gcatcattaa cccagggcga agatccggcg ttcctgaaag aactgctcga atctttatgg
     8881 gtgaaatgca acgacatcgt gctgttgcgc tccaccagta gcgcgcgtta tttcgccggt
     8941 ttcccgaccg gctataccgc actgctcggc gggttaaccg agaacggacg tagcgcggtg
     9001 aacgtacttt cgttcctctg tctcgacgcc tatcaaagtg tgcaattacc gcaaccgaac
     9061 ctgggggtgc gcactaacgc cttgatcgac acgccgttcc tgatgaaaac cgcagaaact
     9121 attcgcctcg gtaccggtat tccgcaaatc tttaacgatg aagtggtggt gccagcgttc
     9181 ctcaaccgtg gcgtttcgct ggaagatgcg cgcgactatt ccgtagtggg ctgcgtggaa
     9241 ttatctattc ccggcagaac ctacggcttg catgacatcg cgatgtttaa cctgctgaaa
     9301 gtgatggaaa tctgcctgca tgaaaatgaa ggcaacgctg cgctgactta tgaaggttta
     9361 ctggaacaga tccgcgccaa gatcagccac tacatcaccc tgatggtaga gggcagcaat
     9421 atttgtgata tcggccatcg cgactgggca cctgtaccgc tgctctcatc gtttatcagc
     9481 gattgtctgg aaaaaggccg cgatattacc gatggcggtg cgcgttataa cttctccggc
     9541 gtacagggga tcggtatcgc caacctgagc gattctctcc atgcgttgaa aggtatggtt
     9601 tttgagcaac agcgtttaag ttttgacgaa ttgctgtcgg tattaaaagc caacttcgca
     9661 acgccagaag gcgaaaaagt ccgtgctcgc ttaattaacc gcttcgagaa atacggtaac
     9721 gatatcgacg aggtggataa catcagcgcc gaactgttac gccactactg caaagaagtg
     9781 gaaaaatacc agaacccgcg cggcggctac ttcacgccgg gatcgtatac cgtttcggct
     9841 cacgtcccgt tgggatcggt ggttggcgcg acgccagacg gtcgttttgc cggggaacag
     9901 ctggcagacg gcggcttgtc acctatgctg ggtcaggacg cacaagggcc aacggcggta
     9961 ctgaagtcag tcagtaagct cgataatacg ctgctgtcta acggtacgtt gctgaacgtg
    10021 aaattcactc cggcgaccct ggaaggtgaa gcgggattac gcaaactggc cgacttctta
    10081 cgggcgttta cccagcttaa gttacaacat attcagttta acgtggtgaa cgccgacacg
    10141 ttgcgggaag cgcaacagcg cccacaagat tatgccgggc tggtggtgcg cgttgccgga
    10201 tacagcgcct tctttgtcga actgtcgaag gagatccagg atgacatcat ccgccggaca
    10261 gcgcatcagc tgtaacgttg tggaaacgcg ccgcaatgat gtggcgcgca ttttcaacat
    10321 tcagcgttat tcactgaatg acggcgaggg cattcgtacg gtggtctttt ttaaaggctg
    10381 tccacatctt tgcccgtggt gtgctaatcc ggaatcgatc tccggcaaaa tccagactgt
    10441 acgcagagag gcgaaatgtc tgcactgtgc gaaatgtttg cgtgatgcgg atgaatgccc
    10501 ctccggggcg tttgaacgga ttggtcgcga tatcagcctt gacgcgctgg aacgggaagt
    10561 gatgaaagat gacattttct ttcgcacgtc cggcggcggc gtcacgcttt ctggcggcga
    10621 agtgttaatg caggcggaat ttgctacccg ttttttacag cgactgcggc tgtggggtgt
    10681 gtcatgtgcc attgaaactg ccggagacgc gccagccagc aagctgttac cgctggcgaa
    10741 attgtgcgat gaagtgttgt tcgatttaaa aattatggac gcgactcagg cgcgggatgt
    10801 ggtgaagatg aacctgccac gcgtgctgga gaatctgcgt ttgctggtga gtgagggcgt
    10861 caacgtgatc ccgcgtttac cgttgatccc tggtttcacg ctcagccggg agaatatgca
    10921 gcaggcgctg gacgtgctga tcccgctgaa tatcaagcag atccatctgt taccgtttca
    10981 tcagtacggc gagccgaaat accgcctgct ggggaaaaca tggtcgatga aagaggttgc
    11041 tgcgccgtcg tcagccgatg tggcaacgat gcgtgaaatg gcagaacggg ccggatttca
    11101 ggttaccgtg ggaggttaaa atggcatacc tggtggcagt aaccgcctgc gtaagcggcg
    11161 tggcgcatac atatatggcg gcggaacggc tggaaaagtt gtgccagtta gagaagtggg
    11221 gcgtcagcat tgaaacccag ggcgcgttgg gaacggagaa tcgtttagcg gacgaggata
    11281 tccgccgggc ggatgttgcg ctgttgatta cggatatcga gcttgccggt gctgagcgat
    11341 ttgaacattg ccgctatgtg caatgcagca tctacgcttt cctgcgtgag ccgcagcggg
    11401 tgatgagcgc ggtacgcaaa gtgctttctg cgccgcagca aacccatctc attctggagt
    11461 agtcggtttt tctgtcagtt ggctgtggta ctgccgacga tattccgacg gtgagcgttc
    11521 ggtattttta cgaaacagac ggcagaagta gttgctgtcg acaaagccgc aggcgtgcgc
    11581 cacttctttt accttcaggt catagccttt caataacgtc ttagcgtgct ccagtcgcgt
    11641 gtgattgagg tattcgttaa aaccaatggc ccccgttttt tgaaacaggt gcgagaggta
    11701 atttggcgaa atatagaatg cctgtgcgac agattcgcgg gtaagcgcgg aggcatagcg
    11761 ttcgtcgata taatcacgaa tagcttcaaa tagtgcctga ctgcgtgagg cggtctggat
    11821 ttggctgcca agcaaatcgc ggcagtggct aagcaggctg gcgacgataa gccttgccgt
    11881 ttgctgctcc tgcggctgca tttgcatttc attgagcgtt tgtagcagaa aagaacctat
    11941 gcgtgggccg cgccgggcga cgtgttgctt cgccagattt tgatattgtt tgccatccca
    12001 ttgcacgacg ctgaagccga gttgctgttt gccaaacagc acgctaaagg tagtggcggg
    12061 ggcttgccat tgtggaaaat tccagccacc agcaggaacg tacagcacat cgccgggaac
    12121 taacgttgct ccagcgccgg tatcaacaaa ctcaccttcc agcacaattt ccagccgtgg
    12181 aaaatcgacc tgatacgcga gatcggggac cggaccgttg ctgctggcaa agtaaatctg
    12241 gcgcagggac agcgggccgt tgatcaggcg ggagagcaga tagctgacgt cgtgatacat
    12301 gttattccct tagctatgta gacgggaaaa cagtaactgg cattatgaaa aaaggccacc
    12361 tgaaaagtgt ggcctgaggg gagttcgatg taaacattgc ggcagtatcg catccggcaa
    12421 atagcgccag acgcgataca aaggtcattt actgattacc tacctggtcg ccatatggca
    12481 gcgtgtcgta atcgatcagc gcgtttttct tgtacgggtt accaatccag cgggtagttt
    12541 ctttgaactg cggattcacc actgaacggg ttggatcgta accgtcgtaa gataagcccg
    12601 ccaaatctga ccaggtgtgg atcagttccg caaggctgta tttacgatca acatcctgcg
    12661 agaaatcacg cggatgagtc gcttgccatt tttccgacgt ccacagcagg aacggaatgg
    12721 tgtacatatg acgcgtcggg ttgtcttcgt tacgcccctg agttttgtgc ggcggcgtgt
    12781 cgtaaacctc ttcaccgtgg tcagagaagt aaaccaggaa accgttcggg tctgctgctt
    12841 taaagtcttt aatcaggctg gcaaccacat gatcgttata caggttagcg ttgtcataat
    12901 cgttatatga ctccagctct tccgcgttta atcccggcgg aacatgatcg gtattgccat
    12961 caaacttgcc ctgattttcc gggtagcggt atttgtattt gatatgcgta cccagcagat
    13021 gaacgatgat cagtttcttc ggcgcagggt cattcagcac ttcctggaac ggcttcagca
    13081 cgttggtgtc gtattcacgc gcactctgcg tacgttgctg gttcatgtag tactgcttgt
    13141 cggtctggcg cgaaaatacc gtcagcatgg tattgcgggc ggtcatcgtc tgctggttgg
    13201 tgatccagaa ggttttataa cccgcctgtt tcatcatgtt catcagcgac ggctgtgtca
    13261 ggtacaggtc cgggttcttt tcattggcaa aggtcagcgc ctgttgcagg atttcaatgg
    13321 tgtacggacg agacgtcacc acgttattaa acacggtcag attcggatcg gttttatgca
    13381 gcgcatccag ctccggcgtg gtttcacgcg gataaccgta cagactcatg cggccacgct
    13441 gggtcgactc gccaatcacc agcactaagg tgcgcggttc gttacccgat tcatctttga
    13501 aattcgccag cggcggcaag gcattatttt cattcagtag ctttgttagc gagtttagtt
    13561 gctgacgata ctgataatag ccggtcagga attgccacgg tgcggcaggc tccatacgcg
    13621 atgccaggtt atccaacgtt ttctcaaacg gcttgttctt gataaacgta ttcatggcga
    13681 tcggatgcag aatcaagcca taaagtaggg caaaagagac aacataacgc cacggctttg
    13741 gaatatagac cgggcgcagg cgtgtccaca gcagaactgc cactgccgta taggccagcg
    13801 cgataagcac aattttcagg ctgaaatact ggcttaaata ctcgctggct tcgttggtgt
    13861 tggtttcgaa catcacaaac agaacgctct gcgagaactc ctgaccgtag atgacgtagt
    13921 agcacagcgc cgccagagag gccgcccata gcaccacgcc gattactgcg gcaataattt
    13981 taatccgctt cggaaagagg aataccggga tcaaccacag cgaactgaat aacagcgagt
    14041 cgcgaatgcc attagtgcca ctataaccac tgatgtaaat aatggcctgt agcagagtag
    14101 agaaaaacca aaagtagagc agtgcccaac ccagggcttt ccagctaaaa agaggtttag
    14161 cctggacttc tgtggaatgc atagtaagaa cctgtcttga aaaaaatatc gccgaatgta
    14221 ccgacaattc cttaaggata tctgaaggga tatccagaat ttgaataaaa tgcagacaga
    14281 aatatattga aaacgagggt gttagaacag aagtatttca gaaaaccctc gcgcaaaagc
    14341 acgagggttt gcaggagggg aagattagcc ggtattacgc atgcctgccg caatcccggc
    14401 aatagtgacc attaacgcct gctcgacgcg aggatccggt tcctggcctt ctttttctgc
    14461 ctggcgggag cggtgcagca actcggcctg caatacgttc agcgggtcgg tgtaaatatt
    14521 ccgtagctga atagactctg caatccacgg cagatcggcc atcagatggg aatcgttggc
    14581 aatcgccagc accactttga tgtcttcttc ttgcagattg cgtaactctt tacctaacgg
    14641 ccacagtgct ttgtctacca gacgttgatc atagtattcc gccagccaca ggtctgcttt
    14701 ggcgaagacc atctccagca tgccgagacg cgtcgagaag aatggccaat cgcggcacat
    14761 agcctccagc tcgctctgtt tgccatcttc gaccactttt tgcagcgccg tacctgcacc
    14821 cagccaggcg gggagcatca ggcggttttg cgtccaggcg aagatccacg gaatggcgcg
    14881 cagtgactca acgccaccgg tcgggcgacg tttggccgga cgtgaaccca acggcagttt
    14941 gcccagttcc tgttccggcg tagcggagcg gaagtaaggc acaaaatctt tgttttcacg
    15001 tacgtagcca cggtagagat cgcaggagat gactgacagt tcatccataa tgcgacgcca
    15061 gctctctttc ggctcaggtg gtggcagcag gttggcttcc agaatcgccc cggtgtacag
    15121 cgacaggctg ctgacggtga tttctggcag accatattta aagcggatca tctcgccctg
    15181 ttcagtcacg cgcaggccac ctttcaggct tcctggcggt tgtgacagca gcgccgcatg
    15241 agcaggtgcg ccgccacgac caatggaacc gccgcgaccg tggaacaacg tcagctcaat
    15301 acccgctttt tcgcaggttt tgattaatgc atcctgtgcc tgatattgcg cccaggaagc
    15361 tgccatcacg cccgcatctt ttgctgagtc ggaatagcca atcatcacca tctgtttgcc
    15421 ctgaatcagg ccgcgatacc agtcgatatt gagcagctgg gtcatgacat cgttggcgtt
    15481 gttcaggtca tcgagggttt caaacagcgg agcaaccggc attgcaaacc cgatacccgc
    15541 ttctttcagc agcaggtgga cagccagcac gtcggacggc gttttcgcca tcgagatcac
    15601 gtaggcggca atggagcctt gcggcgcttc ggcaatcacc tggcaggtat cgagcacttc
    15661 gcgcgtttcg gcgcttggtt gccagttgcg cggcagaagc ggacgtttgg agttcagttc
    15721 gcggatcagg aacgcctgtt tgtcggcctc tgaccagctt tcgtagtcgc cgataccgag
    15781 gtagcgggtc agctcgccca gcgcttcggt atgacgtgtg ctctcctgac ggatatcaat
    15841 acggaccagc ggtacgccga aacatttcac gcggcgcagg gtgtcgagca gatcgccgtt
    15901 ggcgataata cccatgccac acgcctgaag tgactggtag caagcgtaga gcggttccca
    15961 cagttcttcg ttttgtgtca gcaggccttc tggttttggc agttcttcgc ctttcaggcg
    16021 cgcttccagc catgcctgtg tcgccatcag gcgagaacgc aggtttttca tcagatagcg
    16081 atacggttct gcggcacctt cttcgccaac cagcgccagc agttcagggg tcgcttcaac
    16141 catcgacagt tcagaaacca gaacctgaat atctttcagg aacagatcgg tggctttcca
    16201 gcggctgagt agcaggacgt ggcgggtgat atcggcagtg acgtttgggt tgccgtcgcg
    16261 gtcaccgccc atccacgagg taaaacgcac cggaacaaat tcgacgggca gtttgtagcc
    16321 gaggttctct tccagttgtt cgttcagttc gcgcaggtaa ttcggtacgc cttgccacag
    16381 gctgttttct actacggcaa agccccattt ggcttcatct accgggcttg gacgcagctt
    16441 acggatttca tcggtatgcc atgactgggc gatcaactgg cgcaggcggc gcatcagctg
    16501 gttgtgttcg tagtcagcga tatctttgtt atcgagctgt tttaaacagg cgttcacttc
    16561 caccattttg tggatcagtg tacgacgggt aatttcggtt gggtgagccg tgaggaccag
    16621 ttccagcgac agcgattcca ctgctttttt gatggtgtct tcgctcagtt ccggctggtt
    16681 tttcagttta cgcagggtgc gggcgatcac ttccgggttg ctggcagctt cgcctttcgg
    16741 cgaaatgctg tggtattgct cggcggtgtt ggccaggttc aggaactgac taaacgcacg
    16801 cgcaacgggc agcagctcgt cgttcgacaa attttgtaag gtggtgagca actcctggcg
    16861 gttagcatca ttgccagcgc gtgaagattt cgacaactta cggatagttt ctacgcgttc
    16921 aagaatgtgt tctcccaacg catccttgat ggtttctccc agcactttgc cgagcatact
    16981 gacattacta cgcaatgcgg aatattgttc gttcatatta ccccagacac cccatcttat
    17041 catttgatag ccctgtatcc ttcacgtcgc attggcgcga atatgctcgg gctttgcttt
    17101 tcgtcgtctt ttataaagcc acgtaaaagc ggtgacgtca aatgctgcga aatcgcttca
    17161 gcaaacgaat aaatagcagg aatttacgcc attaaattca cgacgcttta aataagcgta
    17221 acttatggga atgttaaaaa atcgtctcaa ttaacaccaa aggtgtaggt cggataagat
    17281 gtgcaagcat cgcatccgac attgtttggc ctacgtttta atgccagcaa aaatggtgaa
    17341 tcacctgggt gatcagttcg cgggtgggct tgataaacct cgtctccaga tattcatcag
    17401 gctgatgagc ctgattaatt gagccaggcc ccagaaccag cgtcgggcat aacgtttgaa
    17461 taaacggcgc ttcggtacag taattcacca cttcggtctt tgctccgagc aatttctcaa
    17521 ccacttcaac cagttgatga ttcggtgggc attcatagcc agggatcggc ggatgcagct
    17581 cgtcgaccgt cagacgaccc ggccagcgtt cgctcactgg agccaatgca tcgttgagca
    17641 aaccattaag ttcattgagt gtcatgcctg gcagcggacg aatatccata tgcaactcac
    17701 agcaagcgca aatacggtta gaagcgtcgc caccgtggat atgcccgagg ttgagcgtag
    17761 ggtatggcac ggtaaacgct tcgtagtgat aacgttcttt caggttatcg cgcaattgca
    17821 aaatatgccc gatggcgtcg tgcattagtt cgatagcgtt aactccgcgt gctggatcgc
    17881 tggagtgccc cgactggccc tgaatacgga tggcgttaga gatatgacct ttatgtgcgc
    17941 gtaccggttg tagtgacgtc ggttcgccaa tgatggcaca atccgggcgc agggcagtag
    18001 tttcggcaaa ataacgcgct ccggccatac tggtttcttc atcggcagtc gccagaatgt
    18061 agagcggttt tttcagtttc gtgacgtcga catcgcgtag cgcatcaagg ataaacgcaa
    18121 aaaagccttt catgtcggcg gtgcctaagc cgtaaagctt gccgtcatgc tccgtcagtg
    18181 taaacggatc gcgcgtccag cgtccgtcat caaatggcac cgtatcggta tgccccgcca
    18241 gcaacaagcc gccagccccc tgtccgcaac tggccagcat attaaatttg ttgcgagttc
    18301 ctggcacagg ctgcacttcc acattgaagc ctaaattttt aaaccagtcc gccagcagag
    18361 tgattaaatc tgcattgctt tgatcgagtg cctcttccgt ggcgcttatt gaaggtgtgg
    18421 caatcagagc acggtaaatc tcgataaatg gcggtaattt gtttttcatt gttgacacac
    18481 ctctggtcat gatagtatca atattcatgc agtatttatg aataaaaata cactaacgtt
    18541 gagcgtaata aaacccacca gccgtaaggt gaatgtttta cgtttaacct ggcaaccaga
    18601 cataagaagg tgaatagccc cgatgttgaa tacgctgatt gtgggtgcca gcggctacgc
    18661 tggcgcagag ctagtgacct atgtaaatcg ccatccgcat atgaacataa ccgctttgac
    18721 tgtttcagcg caaagcaatg atgcgggaaa gttaatctcc gatttgcatc cgcagctaaa
    18781 aggcatcgtt gatctgccgt tgcagccgat gtcggatatc agcgagttta gcccaggggt
    18841 ggacgtggtg tttctcgcca ccgcccatga agttagccac gatttagcgc cgcaatttct
    18901 tgaagcgggc tgcgtggtgt tcgacctttc cggcgcgttt cgtgttaacg acgccacctt
    18961 ctatgaaaaa tattacggct ttacccatca atacccggaa ctgttggaac aggcagccta
    19021 cggtctggcg gagtggtgcg gtaataaatt aaaagaagcg aacttgattg cggtgccggg
    19081 ctgttatccg acggcggcac agctggcgct gaaaccgttg attgatgccg atcttcttaa
    19141 cctcaatcag tggccggtga tcaacgccac cagcggcgtg agcggtgcgg gacgtaaggc
    19201 agcgatttca aatagttttt gtgaagttag cctgcaaccg tatggcgtct ttactcatcg
    19261 ccatcaacca gagatcgcca cacacctcgg tgctgacgtt atcttcaccc cacatctggg
    19321 caatttcccg cgcggcattc tcgaaaccat tacctgccgc ctgaaatcgg gtgtgaccca
    19381 ggcgcaagtc gcgcaagcgt tacagcaggc gtatgcccat aaaccgctgg tgcggctgta
    19441 tgacaaaggc gttccggcgc tgaaaaatgt cgttgggctg ccattttgcg atatcgggtt
    19501 tgccgttcag ggcgagcatt tgattattgt ggcgaccgaa gacaacttac tgaaaggcgc
    19561 ggcggcacaa gcggtacagt gcgccaatat tcgtttcggc tatgcggaaa cgcagtctct
    19621 tatttaaggg tgcaatgatg aatccattaa ttatcaaact gggcggcgta ctgctggata
    19681 gtgaagaggc gctggaacgt ctgtttagcg cactggtgaa ttatcgtgag tcgcatcagc
    19741 gtccgctggt gattgtgcac ggcggcggtt gcgtggtgga tgagctgatg aaagggctga
    19801 atctgccggt gaaaaagaaa aacggcctgc gggtgacgcc tgctgatcag attgacatta
    19861 tcaccggagc actggcggga acggcaaata aaaccctgct ggcatgggcg aagaaacatc
    19921 agattgcggc cgtaggtttg tttctcggtg acggcgacag cgtcaaagtg acccagcttg
    19981 atgaagagtt aggccatgtt ggactggcgc agccaggttc gcctaagctt atcaacacat
    20041 tgctggagaa cggttatctg ccggtggtca gctccattgg cgtaacagac gaagggcaac
    20101 tgatgaacgt caatgctgac caggcagcga cggcgctggc ggcaacgctg ggcgcggatc
    20161 tgattttgct ctccgacgtc agtggcattc tcgacggcaa agggcaacgc attgccgaaa
    20221 tgactgccgc gaaagcagaa caactgattg agcagggcat tattactgac ggcatgatag
    20281 tgaaagtgaa cgcggcgctg gatgctgcgc gcacgctggg ccgcccggtg gatatcgcct
    20341 cctggcgtca tgcggaacag cttccggcac tgtttaacgg tatgccgatg ggtacgcgga
    20401 ttttagctta agttttgtta gccggaggcg cagctttccg gcattaaatt tcaaaataag
    20461 gaaacagagt tatggcactt tggggcgggc gttttaccca ggcagcagat caacggttca
    20521 aacaattcaa cgactcactg cgttttgatt accgtctggc ggagcaggat attgttggct
    20581 ctgtggcctg gtccaaagcc ctggtaacgg tcggtgtgct gaccgcagaa gaacaggcgc
    20641 aactggaaga ggcgctgaac gtgctgctgg aagatgttcg cgccaggcca caacaaatcc
    20701 ttgaaagcga cgccgaagat atccatagct gggtggaagg caaactgatc gacaaagtgg
    20761 gtcagttagg caaaaaactg cacaccgggc gtagccgtaa tgatcaggta gcgactgacc
    20821 tgaaactgtg gtgcaaagat accgttagcg agttgctgac ggctaaccgg cagctgcaat
    20881 cggcgctggt ggaaaacgca caaaacaatc aggacgcggt gatgccaggt tacactcacc
    20941 tgcaacgcgc ccagccggtg acgttcgcgc actggtgcct ggcctatgtt gagatgttgg
    21001 cgcgtgatga aagccgcttg caggatgcgc ttaagcgtct ggatgtcagc ccgttaggct
    21061 gtggcgcact ggcgggaacg gcctatgaaa tcgaccgtga acagttagca ggctggctgg
    21121 gatttgcctc agcgacccgt aacagtctcg acagcgtttc tgaccgtgac cacgtgctgg
    21181 aactgctttc tgctgccgct atcggcatgg tgcatctgtc gcgttttgct gaagatctga
    21241 ttttctttaa caccggcgaa gcggggtttg tcgagctttc tgaccgcgtg acttctggtt
    21301 catcattgat gccgcagaag aaaaacccgg atgcgctgga gctgattcgc ggtaaatgcg
    21361 gtcgtgtgca gggcgcgtta accggcatga tgatgacgct gaaaggtttg ccgctggctt
    21421 acaacaaaga tatgcaggaa gacaaagaag gtctgttcga cgcgctcgat acctggctgg
    21481 actgcctgca tatggcggcg ctggtgctgg acggcattca ggtgaagcgt ccgcgttgcc
    21541 aggaagcggc gcagcagggg tatgcgaacg ccactgaact ggcggattac ctggtggcga
    21601 aaggcgtgcc gttccgcgag gcgcatcata ttgtgggtga agcggtggtg gaagccattc
    21661 gtcagggcaa accgctggaa gatctgccgc tcagtgagtt gcagaaattc agccaggtga
    21721 ttggcgaaga tgtctatccg attctgtcgc tgcaatcgtg cctcgacaag cgtgcggcaa
    21781 aaggcggtgt ctcaccgcag caggtggcgc aggcgattgc ttttgcgcag gcgcggttgg
    21841 ggtaagtcct gggcgattcg tggcctggca aaacgccagg ccatgcttag agggttactg
    21901 cccgtaataa gcattggcac catgcttgcg taaaaaatgc ttatccagta gaggctgttg
    21961 aatagccgta agatgagggt gtagctgttg tgtaaagaga ttcatatagg cgatttcttc
    22021 aagtacaacg gcgttatgca cggcgttcgc gggatcagtt ccccaggcaa aggggccatg
    22081 actgtttact aaaacagccg gaatgtcagc gggagatatt tctcgttgtc tgaacgtttc
    22141 aataatcact cgccccgttt catattcata ttgtccatta atttcgtcat ccttcatggg
    22201 gcgtgtacag gggataatgc cgtagaaata atctgcatgg gttgtcccaa aggccgtgag
    22261 atcttttccg gcctgagccc atattgtggc gtgtcgggaa tgggtatgaa cgatacctcc
    22321 aatatccggg aaagcacggt atagcgccag atgagtatct gtgtctgaag agggtttcct
    22381 gtgtccatca ataacttcac cactttgtaa atccactacc accatatctg caatgcccat
    22441 ttggctgtat tcgaccccag aaggtttaat aaccatgaca cctttttcgc gctcaatacc
    22501 gctaacattt ccccaggtaa acgtcactaa ctgatgaagg ggtagtgata aattcgcagc
    22561 aagaactttt tcctttagcg tatccatttt taactccacg ataatctgta tgtattatat
    22621 aaataaactt taacttttgg gttttggcta tttttgctcg taagttatgt tgcctaaatt
    22681 acgctgaata taagaaatgt aaaatattca aggcatgtaa gaaggcaatg aaagagggta
    22741 agtgggcgta aacggtttta ttcgggcgta ttcggtcatg aatggaagag attgcta
//
LOCUS       DAOTRK010000074        22288 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.074, whole genome shotgun sequence.
ACCESSION   DAOTRK010000074 DAOTRK010000000
VERSION     DAOTRK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.074"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            15..1307
                     /locus_tag="QIE41_002712"
     CDS             15..1307
                     /locus_tag="QIE41_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3531587.1"
                     /translation="MDIPVNAAKPGRRRYLTLVMIFITVVICYVDRANLAVASAHIQE
                     EFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIAIFGWSVATLFQGFAT
                     GLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFYTSGQFVGLAFLTPLL
                     IWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISNAELDYIRDGGGLVDGD
                     APVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLTWFPNYLTQEKGITAL
                     KAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPIICGLLISTCIMGANYT
                     NDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVFNFAGGLGGITVPLVV
                     GYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(1304..2368)
                     /gene="cbrA"
                     /locus_tag="QIE41_002713"
     CDS             complement(1304..2368)
                     /gene="cbrA"
                     /locus_tag="QIE41_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDS3531588.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASFTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMNAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            2469..3683
                     /locus_tag="QIE41_002714"
     CDS             2469..3683
                     /locus_tag="QIE41_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDS3531589.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIVE
                     GGKMSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLCPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(3685..4017)
                     /locus_tag="QIE41_002715"
     CDS             complement(3685..4017)
                     /locus_tag="QIE41_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDS3531590.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    4257..4329
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            4323..4736
                     /gene="ibpA"
                     /locus_tag="QIE41_002716"
     CDS             4323..4736
                     /gene="ibpA"
                     /locus_tag="QIE41_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDS3531591.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            4848..5276
                     /gene="ibpB"
                     /locus_tag="QIE41_002717"
     CDS             4848..5276
                     /gene="ibpB"
                     /locus_tag="QIE41_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDS3531592.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            5472..7133
                     /locus_tag="QIE41_002718"
     CDS             5472..7133
                     /locus_tag="QIE41_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDS3531593.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(7130..7846)
                     /locus_tag="QIE41_002719"
     CDS             complement(7130..7846)
                     /locus_tag="QIE41_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDS3531594.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNANEYQVEYHLRRLHPEKS"
     gene            8142..9758
                     /locus_tag="QIE41_002720"
     CDS             8142..9758
                     /locus_tag="QIE41_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDS3531595.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            9758..11080
                     /locus_tag="QIE41_002721"
     CDS             9758..11080
                     /locus_tag="QIE41_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDS3531596.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGVR
                     QEVIAEACKVILKERAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSAAVAKAILDDLLEANKAYWPELR"
     gene            complement(11077..11970)
                     /locus_tag="QIE41_002722"
     CDS             complement(11077..11970)
                     /locus_tag="QIE41_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDS3531597.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYSDGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            12137..13852
                     /locus_tag="QIE41_002723"
     CDS             12137..13852
                     /locus_tag="QIE41_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDS3531598.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPIFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFSVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            13849..15342
                     /locus_tag="QIE41_002724"
     CDS             13849..15342
                     /locus_tag="QIE41_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDS3531599.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALANIEKPEILPGENILAAKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDLNEMHNLIDDIRFADVRRKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(15389..15838)
                     /locus_tag="QIE41_002725"
     CDS             complement(15389..15838)
                     /locus_tag="QIE41_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531600.1"
                     /translation="MGIIAQNKISSLGMLFGAIALIMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCGLLLMFLIFSFLTGGSLA"
     gene            15946..16293
                     /locus_tag="QIE41_002726"
     CDS             15946..16293
                     /locus_tag="QIE41_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDS3531601.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMI
                     VAVIVMGLVLYAG"
     gene            16283..16645
                     /locus_tag="QIE41_002727"
     CDS             16283..16645
                     /locus_tag="QIE41_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDS3531602.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            16642..17139
                     /locus_tag="QIE41_002728"
     CDS             16642..17139
                     /locus_tag="QIE41_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS3531603.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     HSRRGCQACDWHRLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17147..18331)
                     /gene="emrD"
                     /locus_tag="QIE41_002729"
     CDS             complement(17147..18331)
                     /gene="emrD"
                     /locus_tag="QIE41_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDS3531604.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(18611..18700)
                     /gene="tisB"
                     /locus_tag="QIE41_002730"
     CDS             complement(18611..18700)
                     /gene="tisB"
                     /locus_tag="QIE41_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDS3531605.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            19265..19363
                     /gene="ivbL"
                     /locus_tag="QIE41_002731"
     CDS             19265..19363
                     /gene="ivbL"
                     /locus_tag="QIE41_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDS3531606.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            19469..21157
                     /gene="ilvB"
                     /locus_tag="QIE41_002732"
     CDS             19469..21157
                     /gene="ilvB"
                     /locus_tag="QIE41_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDS3531607.1"
                     /translation="MASSGTTSTHKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIHDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            21161..21451
                     /gene="ilvN"
                     /locus_tag="QIE41_002733"
     CDS             21161..21451
                     /gene="ilvN"
                     /locus_tag="QIE41_002733"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDS3531608.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            21716..>22288
                     /locus_tag="QIE41_002734"
     CDS             21716..>22288
                     /locus_tag="QIE41_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531609.1"
                     /translation="MASPINSGTMMSNPCPSTFLDKNRIIAAELNITNNEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGEDEYIEIK
                     QTGDKSFNITLVGRDKRLRKETPYSGVAVATIIKSLSEKTAALETHSADTVLRKKLVN
                     SIVINNTDFNYEIPAGILSNIYDLLSLRVQK"
BASE COUNT         5292 a         5626 c         5847 g         5523 t
ORIGIN      
        1 ggagaaagct cgctatggat attcccgtta atgcagcaaa gccggggcgt cggcgttatc
       61 tgacgctggt gatgatcttt attacggtgg tcatttgtta tgttgaccgc gctaacctgg
      121 ccgtggcttc cgcccatatt caggaagagt tcggtattac gaaagcggaa atgggctatg
      181 tattttcggc cttcgcctgg ctttatacgt tatgccagat ccccggcggt tggtttttag
      241 atcgcgtagg ttctcgcgtg acttatttta ttgcgatatt tggctggtca gtggcgacgt
      301 tattccaggg ctttgccacg ggattaatgt cattaattgg tctgcgcgcg ataaccggta
      361 ttttcgaagc gcccgctttc ccgaccaata accggatggt gaccagctgg ttcccggaac
      421 atgaacgcgc ttccgccgtt ggtttttata cgtctggtca gtttgtcggt ctggcgtttt
      481 tgactccgct gctgatctgg attcaggaga tgttgagctg gcactgggtg ttcattgtca
      541 ccggtggtat cggcattatc tggtcgctga tttggtttaa ggtttatcag ccgccgcgcc
      601 tgaccaaagg catcagcaac gctgaactgg attacattcg tgatggcggc ggtctggtgg
      661 atggtgatgc gccggtgaag aaagaggcgc gtcagccgtt aacagccaaa gactggaaac
      721 tggtgttcca tcgtaaactg attggcgtct atcttgggca atttgcggtg gcttctacac
      781 tgtggttttt cttaacctgg ttcccgaact atttaaccca ggaaaaagga atcacggcgc
      841 tgaaagcagg ctttatgacc acggtgccat tcctcgcggc gtttgtcggc gtcctgctct
      901 ctggctgggt cgcggatctg ctggtacgta agggcttttc actgggcttt gcgcgtaaaa
      961 cgccgattat ctgcggcttg ctgatctcca cctgcattat gggcgctaac tacactaacg
     1021 atccgatgat gattatgtgc ctgatggcgc tggcattctt cggcaacggt tttgcttcga
     1081 ttacctggtc gctggtctct tctctggcac cgatgcgcct gattggttta accggcggcg
     1141 tgtttaactt tgccggtggt ctgggcggca tcaccgttcc gctggtggtg ggttacctgg
     1201 cgcagggtta cggtttcgcg cctgcactgg tttatatctc cgccgtcgcg ttgattggcg
     1261 cgctctcata catcctgctg gtgggcgatg tgaaacgcgt tggctaatct ttcaactgtg
     1321 gaatatgcgc cacaccactg cgcataatcc actttcttaa agccggtgcg gtcaggcatc
     1381 ggctttttac tattttgccg aagagtttta aacgcagttt gcgggtggcg cgccagtagg
     1441 cggcgttgct cttctccggc tgcttcagta acaccgaacg cagaatctct gcgctatcca
     1501 gcgcatagct aatcccctcc agcgaactgg cgctgataaa tcccgccgct tcaccaatca
     1561 gaaaggcgtt gtccttaccg cagacaaaat cctgccagcg tgagggaaac agcacggtac
     1621 atttctcgct tttcaccgct ttgccaaact ggaactgaaa ggcgttcatt ttctctttca
     1681 gcgtcgtgaa acgcgtctga ccgtctttca tgggataggc accgccaaag ataaaataac
     1741 cgtctttgct gatactccat gagtaacagt cagttatcgc attatcaaag atgcaggagt
     1801 agaacggcac cggatgtttc tctgcgaacc actgctggat agcgacatat ttacgaattt
     1861 gatgatccgg gtagagatgt cgccgcacca tcgagtttgc gccatcggca ccgaccagat
     1921 agcgggcagt aatatgctgc tcccaaccgt ctgcacgaaa aatgacatgc catttatcat
     1981 cctcacgcca gatttttcgg cacaggctgt cgtggtaaac ctcaacgctg gcggggatca
     2041 gcgatttcat ccacaagtcg aaagcatggc gattaatatt gatatagctt cgctggtagt
     2101 tacgcgtgaa cgatgcggca acgtcgacag ttttgacgct gaaaatctgt ggattggcga
     2161 tcacatcgac aggaagcgtc agtccatcgc gaataaaaga tcgctgcgcg tccggtgcca
     2221 gcagaccgcc gcaaggtttg ctgaaacctt cagtaccaca ctggtgcttt ttatccagcg
     2281 cgatcacctg cattttgcct gctaactttc gtgccaacgc cgaccctgcc gggccgaggc
     2341 caataatcgc cacgtcgaaa tgttccatct tttcactcac attcatcacg atggcgaaag
     2401 actattgtct gaaagtgaag tccttgtgaa gtatgatggc gggaaccgtt ttgtatcagg
     2461 accgcgccat gaaacagata acctttgctc cccgtaatca cctgctcacc aataccaata
     2521 cctggacgcc cgacagccag tggctggtat ttgacgttcg tccttcaggg gcgtcgttta
     2581 ccggcgagac cattgagcgt gtgaatatcc ataccggcga ggtcgaggtt atctatcgcg
     2641 cgtcacaggg cgcacacgtc ggcgtggtga ccgttcatcc gaagtcagag aagtatgtct
     2701 tcatccacgg cccggaaaat cctgatgaaa catggcatta cgacttccat catcggcgcg
     2761 gagtgattgt tgaaggcggc aagatgagta accttgatgc gatggatatt accgcgccgt
     2821 atacgccagg agcgctgcgc ggcggcagcc atgtgcatgt ctttagcccg aacggtgaaa
     2881 gggtgagctt tacctataac gaccatgtaa tgcatcaact ctgcccggcg ctggatttgc
     2941 gaaacgtcgg tgttgctgca ccgtttggcc cggtcaacgt acaaaagcag catccgcgtg
     3001 aatacagcgg tagccactgg tgcgtgctgg tgagtaaaac cacgcccacg ccgcagcctg
     3061 gcagcgatga aatcaatcgt gcttatgaag aaggatgggt aggaaatcac gcgctggcgt
     3121 ttattggcga cacactttcg ccaaagggcg agaaagtacc ggagctattt atcgttgagt
     3181 taccgcaaga tgaagctggc tggaaagcgg caggtgatgc gccgttaagt ggaacggaaa
     3241 caaccctgcc cgcgccaccg cgcggcgtcg tgcagcgacg tttaaccttt acccaccatc
     3301 gggcttatcc ggggttagtt aacgtcccgc gccactgggt gcgctgtaat ccgcagggta
     3361 cgcaaatcgc gtttttaatg cgtgatgata acggcattgt gcaactgtgg cttatctcgc
     3421 cacaaggcgg cgagccgcgc cagttaaccc ataacaaaac ggatattcag tctgcattta
     3481 actggcatcc gtcaggagaa tggttgggct ttgtgctgga taatcgaatt gcttgcgccc
     3541 atgcgcaaag cggcgaggtt gagtatttaa ccgaaaacca cgccaatcca ccttctgcgg
     3601 acgccgtggt cttctcgccg gatggtcagt ggctggcgtg gatggaaggc ggccagctgt
     3661 ggatcaccga aactgatcgc taaatcagtt atcaggcatt ctggccggtg gaataaccgc
     3721 attggtggct tgcgcgttgg cttcgctttt ttcaacgcgt gagcgcaccg agctgtcttt
     3781 gcggaacacg tcccacggca gtaaaagcgt gtccatcaca gcggtaaacg gcatatcgag
     3841 aatcgccagc gacttagtgc cccagttggt ttcatcgtcg ccaatcatgg tggcgctggc
     3901 gcgggtgccg ggatacgttc cttctttccc cccggtatgg gacatcacgc tggagcatcc
     3961 tccgagtaat gtcattccgc tgcatatcat gaacgctaac aacacatttc ttatcataat
     4021 aaattcatct gttgatcgtg ggtgttggcc tgatgagtta tagcgatccc ttgctgaaaa
     4081 taacatcatc attacgtcgc actgtggcgg ctatcgcact ttaacgtttc gtgctgcccc
     4141 ctcagtctat gcaatagacc ataaactgca aaaaaaagtc cgctgataag gcttgaaaag
     4201 ttcatttcca gacccatttt tacatcgtag ccgatgagga cgcgcctgat gggtgttctg
     4261 gctacctgac ctgtccattg tggaaggtct tacattctcg ctgatttcag gagctattga
     4321 ttatgcgtaa ctttgattta tccccgcttt accgttctgc tattggattt gaccgtttgt
     4381 ttaaccactt agaaaacaac cagagccaga gtaatggcgg ctaccctccg tataacgttg
     4441 aactggtaga cgaaaaccat taccgcattg ctatcgctgt ggctggtttt gctgagagcg
     4501 aactggaaat taccgcccag gataatctgc tggtggtgaa aggtgctcac gccgacgaac
     4561 aaaaagagcg cacctatctg taccagggca tcgctgaacg caactttgaa cgcaaattcc
     4621 agttagctga gaacattcat gttcgtggcg ctaacctggt gaatggtctg ctgtatatcg
     4681 atctcgaacg cgtgattccg gaagcgaaaa aaccgcgccg tatcgaaatc aactaattcc
     4741 ttaaggccgc ctggcgcggc ctgacatctc catgctcgcc gtcagggagc atatgcgaat
     4801 cttcggattt gcaggtactt actcgcttct tagaaggaga aatgactatg cgtaacttcg
     4861 atttatcccc actgatgcgt caatggatcg gttttgacaa actggccaac gcactgcaaa
     4921 acgccggtga aagccagagc ttcccgccgt acaacattga gaaaagcgac gataaccact
     4981 accgcattac ccttgcgctg gcaggttttc gtcaggaaga tttagagatt caactggaag
     5041 gtacgcgcct gagcgtaaaa ggcacgccgg agcagccaaa agaagagaaa aaatggctgc
     5101 atcaagggct tatgaatcag ccatttagcc tgagctttac gctggctgaa aatatggaag
     5161 tctctggtgc aaccttcgta aacggtttac tgcatattga tttaattcgt aatgagcctg
     5221 aacccatcgc agcgcagcgt atcgctatca gcgaacgtcc cgcgttaaat agctaactag
     5281 ctattctctt tgccccgcca ttctggtggg gctttttttg tctttcttct tcaacaatgc
     5341 gttaattttc gcctgtttaa gatatttcgg cacgttcttt cccgtgataa tgtgcgctat
     5401 aaggcaaatc tgcttcaccg attcagccat aatccgtagc aattaaaatg ttatctcaag
     5461 agaaatggac gatgagtgat atagcattaa cggtcagtat tctggctttg gtggcagtcg
     5521 tcggtttgtt tatcggcaac gtcaaatttc gcggcatagg attaggtatt ggcggcgtgc
     5581 tgtttggtgg gatcatcgtc ggccattttg tttctcaggc ggggatgaca ttaagtagcg
     5641 atatgctgca tgttattcag gaatttggcc tgatcctgtt cgtttatacc atcgggattc
     5701 aggtagggcc gggcttcttt gcctcattgc gcgtctccgg attacgcctc aacctgtttg
     5761 ctgttctgat cgtcatcatc ggtggtctgg ttaccgccat cctgcataaa ctgtttgata
     5821 ttccactgcc ggtagtgctg gggattttct ccggtgcggt aaccaatacg ccagcactgg
     5881 gggcagggca gcagatcttg cgcgacctgg gtacaccaat ggaaatggtc gatcagatgg
     5941 ggatgagtta cgcgatggcg tatccattcg gcatttgcgg gattttgttc accatgtgga
     6001 tgttgcgggt tattttccgc gtcaatgtcg agacagaagc ccagcagcac gagtcttcac
     6061 gcaccaatgg cggcgcgctg atcaggacta tcaatattcg cgttgagaac cctaacctgc
     6121 atgatttagc cattaaagat gtaccgattc tcaatggcga caaaattatc tgctcgcgtc
     6181 tgaaacgcga agaaacccta aaagtacctt cgccagatac cattatccaa ctgggcgatt
     6241 tgctgcatct ggtggggcag ccagcggatt tacataatgc gcaactggtg attggtcagg
     6301 aggtcgatac ctcgctgtct acgaaaggca ctgatttgcg cgtcgagcgt gtggtggtca
     6361 ccaatgaaaa cgtgctcgga aaacgtattc gcgacctgca ctttaaagaa cgctatgacg
     6421 ttgttatctc gcgcctgaac cgtgccgggg tcgaactggt cgccagtggt gatatcagcc
     6481 tgcagttcgg cgatattctc aacctggtgg ggcgtccgtc cgcaattgat gccgttgcca
     6541 atgtgctggg gaatgcgcag caaaaactgc aacaggtaca gatgctgccg gtgtttattg
     6601 gtatcgggct tggcgtattg ttaggttcta ttcccgtctt tgtgccggga ttcccggccg
     6661 cgttgaaact ggggctggcg ggcggtccgc tgattatggc gttgatcctc gggcgtatcg
     6721 gcagtatcgg caagctgtac tggtttatgc cgccaagtgc caacctcgcg ctgcgggagc
     6781 tggggatcgt gctgttcctc tcggtcgttg gtctgaaatc tggtggggat tttgtgaata
     6841 ccctggtcaa tggcgaaggg ctaagctgga ttggatatgg tgccctgatc accgccgttc
     6901 cgctgattac tgttggtatt ctggcgcgga tgttagccaa aatgaattac ctgaccatgt
     6961 gcgggatgct ggctggctcc atgaccgatc caccggcgct ggcgtttgct aataatcttc
     7021 atccaacgag cggtgcggcg gcgctctctt acgccactgt ctatccgtta gtgatgttcc
     7081 tgcgcattat caccccccaa ttactggcgg tgctcttctg gagtatcggt taactctttt
     7141 ccgggtggag tcgccgtaaa tggtactcca cctggtattc gttggcattt ctgaacatta
     7201 ctgaataatt caaaaactcc ccgctctcgc tatatgacaa cgaggtgatc cgcagcagtg
     7261 gcgtgtgttc cgctaccttc atttgccgcg ccagcagtct atcggcgagc accggcgtca
     7321 ggctttcata attaccgcca atcagaatcc cgcactcttg ttcaatatat tcaaacttcg
     7381 acccttccag atgttgcagc gaaaggttac ggaacagttt taccggcata tagctgtctt
     7441 ccagcatcag cggtttccct tccacaaaac gaacgcgacg ggagaagtag atctgctcgt
     7501 tgatttgaat ccgtaactgg ctggcaatgg ccggaggcgc aggcatgatt tcaaaaatca
     7561 gcacctggct ggtgaccgtt tttccctggc gttttaacac ctccaccagc ccggtcaggc
     7621 tggcggtttg atgcaataca tctttacgca ccaggtaagt accgctgccg tggcggcgga
     7681 ccaccagccc ccacgctacc agcagatcaa tggctttacg gatggtcatc cgcgataccg
     7741 caaactcttc cgccagcttt ttttcaccgg gaagaaggct atttagcgtg aaatccgcgg
     7801 agttaagtcg aattcttaac cgctccgcaa tgcttttgta gatcatctct agatctcttt
     7861 tttgcctgca agcccgggta atgcgcccac aagttctgct taatcgattg aaaaaaaatc
     7921 ttttttttaa agatgtgttc gatgctgtgt accagtgctc acagatgtct actttttcgc
     7981 gaaaacgtag atctctaccg cccaacgaaa agcatgaaag cgatcacgaa tcccattggg
     8041 tcgtcatgtt ctgccgatcg catcttccta tcctcgctcc aggcctgccg cataaccaat
     8101 caggcttcct acttacagaa ttgagaaaag aggatgtgga aatgctcagt caaattcaac
     8161 gctttggcgg cgcgatgttc acgccagtgc tgctgtttcc cttcgccggg attgtggtgg
     8221 gtcttgccat cttgctgcaa aacccgatgt ttgtcgggga atcactgacc gatccgaaca
     8281 gtttattcgc gcaaatcgta cacattattg aagagggcgg ttggacggta ttccgtaata
     8341 tgccgctgat ttttgctgtc ggtttaccca ttggccttgc gaagcaagcg caggggcgtg
     8401 cttgtctggc ggtgatggtg agtttcctga cctggaacta tttcatcaac gcgatgggaa
     8461 tgacctgggg aagctacttc ggcgtcgatt tcactcagga cgcggtggca ggtagcgggc
     8521 tgacaatgat ggcggggatt aaaaccctcg ataccagcat tatcggcgcg attataattt
     8581 ccggcattgt gacggcgctg cataaccgtc tgttcgataa aaaactgccg gtgtttctcg
     8641 gcattttcca ggggacatct tatgtggtga ttatcgcctt cctggtgatg atcccctgcg
     8701 cctggctgac gttgctcggc tggccaaaag tacaaatggg gattgaatct ctgcaagcgt
     8761 tcctgcgttc ggcgggtgcg cttggggtct gggtttacac cttcctcgaa cgtattctga
     8821 tcccaaccgg tttacaccac ttcatctacg gaccgtttat ctttggtccg gcagctgttg
     8881 aaggcggcat tcagatgtac tgggcgcagc atctgcaaga gttcagtctg agcgccgagc
     8941 cgctgaaatc gttgttcccg gaaggcggtt ttgccctgca cggtaactca aaaatctttg
     9001 gtgccgtggg catttcttta gcgatgtact tcactgccgc accggaaaat cgggtaaaag
     9061 tggcgggctt gctgattccc gcaaccttaa ccgccatgct ggtgggaatt accgaaccgc
     9121 tggaatttac cttcctgttc atttcaccgt tgctgtttgc ggtacacgct gtgcttgcgg
     9181 cctcaatgtc gaccgtgatg tacctctttg gtgtggtggg caacatgggc ggtggtttga
     9241 ttgaccaggt tttaccgcaa aactggatcc cgatgttcag caaccacgcg gatatgatgc
     9301 tgacccaaat cgccattggg ttgtgcttta ccctgctgta cttcgtggtt ttccgcaccc
     9361 tgattctgca attcaacatg tgcacgccgg gacgtgaaga tgcggaagtg aaactctact
     9421 caaaagccga atacaaagcc tcgcgaggcc aaaccaccgc ggcagagcca aaaaaagagc
     9481 tggatcaggc tgccggtatc ctgcaagccc tgggcggggt cggcaatatc tccagcatta
     9541 acaattgcgc gacgcgtcta cggattgcac tgcatgacat gtcacaaacg ctggatgacg
     9601 aagtctttaa aaagctggga gcgcacggcg tcttccgtag tggcgatgcc attcaggtga
     9661 tcattggtct gcatgtatcc cagctacgtg aacagctcga tagcttaatt aattctcatc
     9721 aatcagcaga aaatgttgcc attacggagg cagtataatg accaaattct cagtggttgt
     9781 cgcaggcggt ggaagcacct ttacgccagg catcgtgttg atgctcctgg cgaatcagga
     9841 ccgtttcccg cttcgtgcac tgaaatttta tgataacgat ggtgtgcggc aggaagtgat
     9901 tgctgaagcc tgtaaagtca tccttaaaga gagagcgccg gacattgcgt ttagttacac
     9961 caccgatcct gaagtggcgt tcagcgacgt tgattttgtc atggcgcaca tccgcgtcgg
    10021 caaatacccg atgcgcgaac tggatgaaaa aatcccgctg cgccacggcg ttgttggaca
    10081 ggaaacttgc ggacccggcg gaatagcgta cggcatgcgt tccattggcg gcgtcctgga
    10141 actggtggat tatatggaaa aatattcacc aaatgcctgg atgctcaact actccaaccc
    10201 ggcagccatt gtcgcagaag ccacgcgtcg tctgcgcccg aatgcgaaaa tcctcaacat
    10261 ctgtgacatg ccaatcggta ttgaaagccg gatggcgcaa attgttgggc tgcaagatcg
    10321 caaacagatg cgcgtgcgct actacggcct gaaccacttt ggctggtgga catcaattga
    10381 agatttgcag ggcaacgacc tgatgcccca gctgcggcaa tatgtctcga agcacggtta
    10441 tgttccaccg cagcaagata cacatactga agcgagctgg aacgacacct atgcaaaagc
    10501 gcgggatgtc caggcactgg acccggatac attgccgaac acctatctga aatattatct
    10561 cttcccggat tatgtcgttc agcattccaa ccctgaacat acccgcgcga atgaggtgat
    10621 ggagcatcgc gagaaacagg ttttcgatgc ttgccgcgcc attacggcgg caggaaattc
    10681 agcggcgggc aagctggaaa ttgacgaaca tgcgtcatac atcgtcgatc tggcggcggc
    10741 aattgccttc aacactcagg agcggatgtt gctgattgtg cctaacaacg gggcaatcca
    10801 taactttgat gatgaagcga tggtcgagat cccgtgtctg gtcgggcaca acggaccaga
    10861 accactggtg gtcggcgata tcccgcagtt tcagaaaggg ttaatgagtc agcaagtggc
    10921 ggtggaaaaa ctggtcgtgg acgcctggga acagcgttca tatcagcacc tgtggcaggc
    10981 gattactttg tcgaaaacgg taccgagcgc cgcggtcgcc aaagctattc tggatgattt
    11041 gctggaggcc aacaaagcgt actggccaga gttacgttaa ttgtgtggac tggcatccgt
    11101 tgggtgctgg tccgtattct ttagcacggc tgacggcgca taaccaaatt tatgtttaaa
    11161 ggctttgctg aaatgaaagg agtcgaagaa gttgagcgta tcagcgactt cgccaaccga
    11221 gttcccttgt tgctttaata gcgatagcgc cagatccaga cgtgcatcga ggtaatattc
    11281 tttcggcgtc ttgccggtat agcgtaaaaa cagacgacgc aaccaggctt cgctacaggg
    11341 gatcgtggca gccatatcag ctacgctcca gcgttgttgc agactggcat gtaaagtggc
    11401 aattagtttt tcaatttgcc gccgttgtgg atctttttta ccgtctgagt acataagaca
    11461 aatccactga taaataattt tcgtcagaaa agcgactgcc agattatttt ttattgcttc
    11521 tggtgaagta attaactcag caacttcggt gagttcctga ttataaattt cgccattata
    11581 aataacgctt tgctgaccga cgggaatatc catcatactg gtgggggtaa attccatcca
    11641 gtactgttcc cagactaaac cttcacagtg ataagagtga atatccattg gctttaaaaa
    11701 tataatacag ttgccatgca gagttatttg ttcaccattc tttaaaagaa ttttcccaca
    11761 tccttgtacg gtatacaccg caacccatga gttggcgata agcggtttct tcttgtcccg
    11821 cggccagatc acccggtaat gctcgtcggc gtaggtatgc cagagcgaga tcagagaaat
    11881 tccgctggag atcacatttt cgttaatcaa taagggaata ttgtacggag tttcgttttt
    11941 tacatctgaa ctttgcaatt ttccattcat tttaatatcc ctccggcgaa ttatgctcac
    12001 aacctgaact taacagaaaa caatccgttc agggatacga cgtttgtatt ttaagaatct
    12061 gactgcctga cccgacgcat tttacctctc cctatattca tgcgtccggg acataaacat
    12121 aaataagggc tatgagatga attcgttaca aatcttgagt tttgtcggtt ttacgctgct
    12181 ggtggcgatc atcacctggt ggaaggttcg caaaacagat accggatcgc aacaaggcta
    12241 ttttcttgcc ggacgttcac taaaagcgcc ggttattgcc gcttcgttaa tgttaaccaa
    12301 cctttccacg gaacaactgg ttggactttc cgggcaggcc tacaaaagcg gcatgtcggt
    12361 gatgggctgg gaagtgactt cagcggtgac gctgatcttc ctcgcgctaa tctttttacc
    12421 gcgctatctg aagcgcggca ttgccaccat ccccgatttt ctggaggaac gttatgataa
    12481 aacgacgcgt attatcatcg acttctgctt cctcattgcc accggcgtct gctttctgcc
    12541 gattgttctc tactccggcg cgttggcgct caacagcctg tttcacgtcg gggaatcgct
    12601 acagatttct cacggtgcgg ctatctggct attggtaatt ttgcttggtc tggcgggaat
    12661 tttgtatgcg gtgatcggcg gactgcgcgc aatggcagtg gcggactcca tcaacggtat
    12721 tggtctggtt atcggcgggt tgatggtgcc gatatttggc ctgatcgcga tgggcaaggg
    12781 cagctttatg cagggcattg agcaactcac caccgttcac gccgagaaat taaactcagt
    12841 cggcggcccg accgatccct tgccgattgg cgcggcattt accggtttaa ttctggtgaa
    12901 caccttttac tggtgtacaa atcagggcat cgtgcaacgc acgctggcgt caaaaagcct
    12961 ggcggaaggg caaaaggggg cgctgttaac ggcggtgctg aaaatgctcg acccgctggt
    13021 actggtgctg ccagggttga ttgcgtttca tctgtatcag gatctaccga aagccgacat
    13081 ggcctacccg acgctggtca ataacgttct gccagtgcca ctggtgggtt tcttcggcgc
    13141 ggtgttattt ggtgcggtga tcagtacctt caacggcttt ctgaatagcg ccagtacgtt
    13201 attcagtatg ggtatttacc gtcgcatcat taaccagaat gccgagccgc aacagctggt
    13261 caccgttggg cgcaaatttg gtttctttat cgccatcgtt tcggtgctgg tcgcgccgtg
    13321 gattgccaac gcgccgcagg ggttgtatag ctggatgaaa cagctcaacg gtatttacaa
    13381 cgtgccgctg gttaccatca tcattatggg ctttttcttc ccgcgcatcc cggcgctggc
    13441 ggcaaaagtg gcgatgggga ttggcataat cagctacatc accatcaact atctggtgaa
    13501 gttcgacttc catttcctct atgtgctggc ctgtacgttc tgcatcaacg tggtcgtgat
    13561 gctggtgatc ggttttatca aaccgcgcgc cacgccgttc accttcaaag atgcgttttc
    13621 agtggacatg aaaccgtgga aaaacgtcaa gatcgcgtca attggcatcc tgttcgcgat
    13681 gattggcgtc tatgccgggc tggctgaatt cggcggctac ggtacgcgct ggttagcgat
    13741 gatcagttat ttcattgccg ccgtagtgat tgtctacctg atttttgaca gctggcggca
    13801 tcgtcacgac ccagccgtaa cctttactcc cgacgcgaag gatagcctat gaaacgcccc
    13861 aattttctgt tcatcatgac cgatacccag gccaccaata tggtcggttg ctatagcggt
    13921 aagccgctga atacgcaaaa tattgatagt ctggcggcgg aaagtattcg ctttaattcc
    13981 gcctacacct gttcaccggt ttgtacacca gcgcgcgccg ggctgtttac cggtatctac
    14041 gctaaccagt ccggcccgtg gaccaacaac gtcgcgccgg gcaaaaacat ctccactatg
    14101 gggcgctact ttaaggatgc cggctatcac acctgttaca tcggcaaatg gcatctcgac
    14161 ggtcatgact atttcggcac tggcgagtgt ccgccggagt gggacgctga ttactggttc
    14221 gatggggcga actatcttag cgaactgacg gaaaaagaaa ttagcctgtg gcgcaatggc
    14281 ctaaacagcg tcgaagattt acaggcgaac catattgacg aaaccttcac ctgggcgcac
    14341 cgcatcagca atcgagcagt ggattttctg caacagcccg cgcgcgccga ggaacccttc
    14401 ctgatggtgg tttcgtatga tgagccgcat cacccgttca cctgtccggt ggagtattta
    14461 gagaaatacg ctgattttta ctacgagctg ggcgagaaag cacaggatga cctggcgaac
    14521 aaaccggaac atcaccgctt atgggcgcag gcgatgccat cgccagtcgg tgatgacggg
    14581 ctttatcacc atccgctcta ttttgcctgc aatgactttg ttgatgacca aatcggacgt
    14641 gtcatcaatg ccttaacgcc agagcaacgt gaaaatacgt gggtcattta tacctccgat
    14701 cacggtgaaa tgatgggcgc acataagctg atcagtaaag gagcggcgat gtatgacgac
    14761 atcacccgta ttccgctgat catccgttcg ccgcaagggg agcggcgaca ggtcgatacg
    14821 ccagtcagtc atatcgattt actgccgaca atgatggcgc tggcaaatat tgaaaaacca
    14881 gagattctgc cgggggaaaa tatccttgcc gcgaaagagc cacgcggcgt aatggtggaa
    14941 tttaaccgct acgagattga gcatgacagc tttggcggtt ttattccggt gcgttgctgg
    15001 gtgacggatg actttaaact ggtactcaac ctcttcacca gtgatgaact ttacgatcga
    15061 cgtaatgacc taaatgaaat gcataatctg atcgatgata tccgttttgc cgacgttcgc
    15121 cgcaaaatgc atgacgcctt attggattac atggacaaaa ttcgcgatcc gttccgcagt
    15181 tatcaatgga gcctgcgtcc gtggcgtaaa gatgcacagc cgcgctggat gggggcattt
    15241 cgtccacgcc cacaagatgg ctattcgcca gtggtacgcg actatgacac cggcctaccg
    15301 acgcaaggag tgaaagtgga agagaaaaaa cagaagttct gacgataatg cccgctgatg
    15361 atcacccggc gggcattatt caggctgctt aagccagcga accgccggtc aggaaagaaa
    15421 aaatcaggaa catcagcaga aggccgcaca cgatgccgat accaaaaagc aaggtatgga
    15481 aagccagcgt cccgccgccg aaaaccagcg cgccgcgaat gccccagcga ttttccttac
    15541 gcataccgcc aacaaatgcg caaagtagcg cagcaatggc cagtgcgata ccgctttgat
    15601 cgaggatttt atcgacatcc acgtcctctt ttttctccac ggtggtaatt ttttcgcctt
    15661 ttattccggc gagaagacct cttttaattt cagccgtttt gtccgccacg atgctttcaa
    15721 gcgttggcgg tggtgcggaa aaagggccga atgaaaagtg gataatcccc attattaaag
    15781 cgatggcacc gaacagcatg cccaatgaac tgattttatt ttgcgctatg atacccatct
    15841 gacctccttg tagcggatgg caaaagcata tcaggaaacg ggtggacgaa attcttaaaa
    15901 aaaccgctaa aggtagagcg ttagtgaaat tgcaaaggag caacaatgaa aatttcccgc
    15961 ctcggagaag cgcccgatta ccgcttctcg ctggctaatg agcgtacttt tctggcgtgg
    16021 atccgtaccg cactaggatt tctggctgcg ggggttgggc ttgaccaact tgcgccagat
    16081 ttcgccacgc ccgtcattcg cgaactgctg gcgttgttgt tgtgtctgtt ttccggcggg
    16141 ttagcgatgt atggctactt gcgctggtta cgcaatgaaa aagcgatgcg tctgaaagag
    16201 gacctgcctt acaccaacag cttattaatc atcagtttaa tattgatgat cgtggcggtg
    16261 atcgtcatgg gactggtgtt gtatgccgga tagccgcaaa gccagacgca ttgccgaccc
    16321 agggctgcag ccggagcgca catcactggc gtggtttcgt accatgctgg gctacggcgc
    16381 gttgatggcg ttggctatca aacacaactg gcaccaggcg ggcatgttat tctggatttc
    16441 gattggcatc ctcgccatcg tggcgctgat cctctggcac tacactcgta atcgcaattt
    16501 aatggatgtc acgaatagcg atttttccca atttcacgta gtccgtgaca aatttttgat
    16561 ctccctcgcg gtgttatctc tcgcaatact gtttgctgta acgcatatac atcaacttat
    16621 cgtatttatt gagagagtcg catgacagga agtcaggtca ttgatgctga agaggacagg
    16681 cataaactgg ttgtagagta taaggatgct ctgcaacctg ccgattttta tcacaatttc
    16741 aaacagcggg gcatccgctc cgtacagctc attccccatc ttgaatttga tgaccggggc
    16801 gatctgacgg ccgcttcggt gacagcagag ctgtggggga aatttttaat tgccctgttt
    16861 gagtgttggg tgcgggcaga tatcagtcgt atctcgattg agctttttga cgccacatta
    16921 caaaaatggt gtggcagcga aaacccgcac tcgcgccgtg gctgccaggc gtgcgactgg
    16981 catcggctgt gcccgcacgc tcgggaggag acgccagaca gcgtgctatg tgcgggctat
    17041 caggcgtttt attcttactc cgcaccgcat atgcgggtga tgcgtgatct gataaagcag
    17101 catcgttctc ccatggagct gatgacgatg ctgcggtgac gtgagcttaa acaggctgcc
    17161 cctgatgcga catccgcgtc gccagcggta gccagcacag cacgatcaac aatcccatta
    17221 aggtcatcaa caaccccagg ctgccctgac cggtttgcgg caacatcgca gagagcgacg
    17281 ccagcacgcc ggaaccaatg ttttgcagac cgccgaccag cgcgcccgcg gtgcccgcca
    17341 ggaaggggaa cggttccatt gcgccgctgg tcgccagcgg aaacagcatc ccggcaccga
    17401 aaaagaacag cgcggcggga acgagcagcg tccagacatt catcacgcca aaccagtcgg
    17461 ggatccacat cagcaagcca gccagcaggc agcagataac cgactgccac atcaacgttg
    17521 agaagcgttt attaggacgt ccggcaaacc atgccccaaa aaatgccgcc ggaatcggca
    17581 gaataaacaa aatactgacc gtcatactgc tcagccctaa taccgcgccc atcagcacgc
    17641 cggagcaggc ttcaaaggcg gcaatcccgg ccagaccgcc aatcagcatc agcaaataac
    17701 agttaaaacc gctgttaccg aaaagcgttt tataactggt aagcaggcgt gtgcgcggtg
    17761 cgtcgaccgg acgcgtttcc ggcatccagc gggccatact gaaggtcaca ccggcacaaa
    17821 gtaccaacaa aaagagataa caggcgcgcc agttccacat cgtatccagc agaccgccga
    17881 ttagcggtgc gagcaacgga ctgacaagaa ttcccatgtt taacaggcta ttagcatggc
    17941 gcaattgtgt ccgttcatat aaatcacgcg gcaaagtacg cgccattacg ccgccaacgc
    18001 cggtgcccat cccctgcatc gcgctggcgg caatcaatac tgtcaaactg gaggtcgtga
    18061 ccgcgaccag cgttgccagc ataaaaatgg acattccgac gaggatcacc ggtcggcgac
    18121 ccacacggtc ggaaatcggg ccataaaaca gctgtgagac accgtaagtc agcagataag
    18181 cgcccattac gctctgcacc gccccttcac ggacgttgag atcgcgcgcc atatcggcaa
    18241 tagctggaat ataaatggtt tgcgccatct gaccgacggc cacgagtaat accaacatca
    18301 ataacaaatt gacgtttcta tgccttttca ttatcacgga tgcttttata aaaataatta
    18361 gaataagtca ctacgcattc ccataaatgc gggagaggaa tctatcacat tgataaatgt
    18421 tagccagata tacgccagga atggggaatt gtttagcggc agaatatgta aacaaaagcg
    18481 gcaataaatg ttgccgggag ggatggtagg gcggataaga tgcatcagca tcgcatccga
    18541 caccaacccg cacgctaaat atgaaagggg agcggttacc cgctcccctt tggtgcgact
    18601 tgaatctgaa ttacttcagg tatttcagaa cagcatcaag cagttgcagt gctgcaacaa
    18661 tgagtttgag gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag
    18721 gagcactgcc agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat
    18781 ctcaaggaga aacgttgtgc tcgttgacat aacacagtgt gctcacgcgg ggttaaggcg
    18841 ctgacggcac cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc
    18901 ataacacatt gcgtacagtg atattataga agtttactgt ataaataaac agtaatattt
    18961 agacaaaacg caaactgtga tcgatactca actatgcact aaatacgtca aaattcgtgc
    19021 cgaaattgcg cgttttgcgc ggaacacgta tactttctgt gttgacataa tacagtgtgc
    19081 tttgcggtta ccagccgcgg gcggctgacg aaacctcgct ccggcggggt tttttgttac
    19141 ctgcagtaca gtacaaaacg tgatcaaccc ctcaattttc ccttgctgaa aaattttcca
    19201 ttgtctcccc tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc
    19261 cccaatgact acttccatgc tcaacgcaaa actactacca actgcgccat ccgccgcagt
    19321 ggtcgtcgtg cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg aacaacacac
    19381 gattccaaaa ccccgccggc gcaaaccggg cggggttttt cgtttaatca cctcccggaa
    19441 agtcggccca gaagaaaagg actggagcat ggcaagttcg ggcacaacat cgacgcataa
    19501 gcgctttacc ggcgcagaat ttatcgttca tttcctggaa cagcagggca ttaagattgt
    19561 gacgggcatt ccgggcggtt ctatcctccc tgtttacgac gccttaagcc aaagcacgca
    19621 aatccgccat attctggctc gccatgaaca gggcgcgggg tttatcgctc agggaatggc
    19681 gcgcaccgac ggtaaaccgg cggtctgtat ggcctgtagc ggaccggggg cgactaacct
    19741 ggtgaccgcc attgccgatg cgcggctgga ctccatcccg ctgatttgca tcactggtca
    19801 ggtacccgcc tcaatgatcg gcaccgacgc cttccaggaa gtggacacct acggcatctc
    19861 tatccccatc accaaacaca actatctggt cagacatatc gaagaactcc cgcaggtcat
    19921 gagcgatgcc ttccgtattg cgcaatcagg ccgccctggc ccggtgtgga tagacattcc
    19981 taaggatgtg caaacggccg tttttgagat tgaagcacag cccgcggtgg cagaaaaagc
    20041 cgtcgcgccc gcctttagcg aagaaagcat tcatgacgca gcggcaatga ttaatgctgc
    20101 caaacgcccg gtgctttatc tgggcggtgg tgtgatcaat gcgcctgcgc gagtgcgtga
    20161 actggcggag aaagcgcagc tgcctaccac catgacttta atggcgctgg gtatgctgcc
    20221 aaaagcgcat ccgctgtcgc tgggtatgct ggggatgcac ggtgtgcgca gcaccaacta
    20281 tatcttgcag gaggcggatc tgctgattgt tctcggcgcg cgttttgatg accgggcgat
    20341 tggcaaaacc gagcagttct gtccgaatgc gaaaattatt catgtcgata tcgatcgcgc
    20401 agagctgggt aaaatcaagc agccgcacgt agcgattcag gcggatgttg atgacgtgct
    20461 ggcgcagctg atcccgcagg tggaagcgca accgcgtgca gagtggcacc agttggtagc
    20521 ggatttgcag cgcgaattcc cgtgtccaat cccgaaagcg tgcgatccat taagccatta
    20581 cggcctgatc aacgccgttg ccgcctgtgt cgatgacaat gcgattatca ccaccgatgt
    20641 gggtcagcat cagatgtgga cggcgcaggc ttatccgctc aatcgcccac gccagtggct
    20701 gacctccggc gggctgggca cgatgggctt tggtctgcct gcggcgattg gcgcggcgct
    20761 ggcgaacccg gatcgcaaag tgttgtgctt ctccggcgac ggcagcctga tgatgaatat
    20821 tcaggagatg gcgaccgcca gtgaaaatca gctggatgtc aaaatcattc tgatgaacaa
    20881 cgaagcgctg gggctggtac atcagcaaca gagtctgttc tacaagcagg gcgtttttgc
    20941 cgctacctat ccgggaaaaa tcaactttat gcagattgcc gccggattcg gcctcgaaac
    21001 ctgtgatttg aataacgaag ccgatccgca ggctgcattg caggaaatca tcaatcgccc
    21061 tggtccggcg ctgatccatg tgcgcattga tgccgaagaa aaagtgtacc cgatggtgcc
    21121 gccaggtgcg gcgaatactg aaatggtggg ggaataagcc atgcaaaaca caactcatga
    21181 caacgtgatt ctcgaactca ccgttcgcaa ccatccgggc gtaatgaccc acgtttgtgg
    21241 cctttttgcc cgtcgcgctt ttaacgtcga aggcattctc tgcctgccga ttcaggacag
    21301 cgacaaaagc catatctggc tactggtcaa tgacgaccag cgtctggagc agatgataag
    21361 ccaaatcgat aagctggaag atgtggtgaa agtgcagcgt aatcagtccg atccgacaat
    21421 gtttaacaag attgcggtgt tttttcagta aggagcgaca ttaaaccgct cggggggttt
    21481 actgatgagc gatttttgat agatccgttc agatatatag ggtgaaattg tgccagagac
    21541 catctcaggc acaatttatt agcccaggac gttaatttaa gtcgctgcac cgataaccag
    21601 ggggttgaca gttagggcta aaaatttcca gcgatggtgt taaattctga ttgctgtcta
    21661 taccagtggt catgaccagc attctattaa ttaatggttc atcgtgaggt atattatggc
    21721 aagccctata aacagcggaa caatgatgtc aaatccctgc ccctccacct ttttggataa
    21781 aaatagaatt attgctgctg agcttaatat cacaaataac gagaaaaaat attcacctgg
    21841 cagtaatttc gcaaaatgga tgttacagga aataaaacgc ttaatagcaa atattatgtc
    21901 cggttctcgc agtgttaata ccgaaatact taactatttt caccctatgc caggcactga
    21961 aaataatggc aatcgtacct ggatggcggc aactggagag gatgagtata tcgaaatcaa
    22021 acaaacaggt gataagtcat ttaatattac tctggttggt agagataaac gtttacgaaa
    22081 agaaactcca tattcaggag tggctgtagc gactattatc aaatcgttgt ctgaaaaaac
    22141 tgctgcgtta gaaacacatt ccgctgacac ggttttgaga aagaaattag ttaatagtat
    22201 tgttatcaat aatacagact ttaattacga aattcctgcg ggaattttaa gtaatatata
    22261 tgatttatta agcctgcgag ttcaaaaa
//
LOCUS       DAOTRK010000075        22175 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.075, whole genome shotgun sequence.
ACCESSION   DAOTRK010000075 DAOTRK010000000
VERSION     DAOTRK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.075"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..548)
                     /gene="mocA"
                     /locus_tag="QIE41_002735"
     CDS             complement(<1..548)
                     /gene="mocA"
                     /locus_tag="QIE41_002735"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDS3531610.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVKTEYCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNITNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDF"
     gene            651..1421
                     /gene="yqeC"
                     /locus_tag="QIE41_002736"
     CDS             651..1421
                     /gene="yqeC"
                     /locus_tag="QIE41_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDS3531611.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            1469..3094
                     /locus_tag="QIE41_002737"
     CDS             1469..3094
                     /locus_tag="QIE41_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDS3531612.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3135..4067)
                     /gene="arcC"
                     /locus_tag="QIE41_002738"
     CDS             complement(3135..4067)
                     /gene="arcC"
                     /locus_tag="QIE41_002738"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDS3531613.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4115..5500)
                     /gene="hyuA"
                     /locus_tag="QIE41_002739"
     CDS             complement(4115..5500)
                     /gene="hyuA"
                     /locus_tag="QIE41_002739"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDS3531614.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNIREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5553..6764)
                     /locus_tag="QIE41_002740"
     CDS             complement(5553..6764)
                     /locus_tag="QIE41_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDS3531615.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6822..8018)
                     /gene="dpaL"
                     /locus_tag="QIE41_002741"
     CDS             complement(6822..8018)
                     /gene="dpaL"
                     /locus_tag="QIE41_002741"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDS3531616.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALNDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8076..9263)
                     /gene="ygeW"
                     /locus_tag="QIE41_002742"
     CDS             complement(8076..9263)
                     /gene="ygeW"
                     /locus_tag="QIE41_002742"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDS3531617.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9742..11520
                     /locus_tag="QIE41_002743"
     CDS             9742..11520
                     /locus_tag="QIE41_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDS3531618.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVISITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(11560..12039)
                     /gene="xdhC"
                     /locus_tag="QIE41_002744"
     CDS             complement(11560..12039)
                     /gene="xdhC"
                     /locus_tag="QIE41_002744"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDS3531619.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGMPLSELLRDQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12036..12914)
                     /gene="xdhB"
                     /locus_tag="QIE41_002745"
     CDS             complement(12036..12914)
                     /gene="xdhB"
                     /locus_tag="QIE41_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDS3531620.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRLLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRSVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDSGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(12925..15222)
                     /gene="xdhA"
                     /locus_tag="QIE41_002746"
     CDS             complement(12925..15222)
                     /gene="xdhA"
                     /locus_tag="QIE41_002746"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDS3531621.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAEPIHNGGN
                     LLKQSTMTTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTIDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFIEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            15638..16390
                     /locus_tag="QIE41_002747"
     CDS             15638..16390
                     /locus_tag="QIE41_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDS3531622.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            16469..16542
                     /locus_tag="QIE41_002748"
     tRNA            16469..16542
                     /locus_tag="QIE41_002748"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16501..16503,aa:Gly,seq:ccc)
     gene            16763..18412
                     /locus_tag="QIE41_002749"
     CDS             16763..18412
                     /locus_tag="QIE41_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531623.1"
                     /translation="MESFMKGKSTLTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STPDQIKRMQDVISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWEKGDRLKSIALLVAYLDKANFYIMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFISDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAI
                     VYKESRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHE
                     APSPIIPLNWSKASMTLMLKEMSNLIMMREINSFHNEAVLD"
     gene            18450..18629
                     /locus_tag="QIE41_002750"
     CDS             18450..18629
                     /locus_tag="QIE41_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HDS3531624.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAVITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            18975..19724
                     /locus_tag="QIE41_002751"
     CDS             18975..19724
                     /locus_tag="QIE41_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3531625.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVERERHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
     gene            19721..21424
                     /locus_tag="QIE41_002752"
     CDS             19721..21424
                     /locus_tag="QIE41_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HDS3531626.1"
                     /translation="MKIKLRITIILISALCIFNGLLTPGAYAATANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFASRNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            21429..>22175
                     /gene="sctW"
                     /locus_tag="QIE41_002753"
     CDS             21429..>22175
                     /gene="sctW"
                     /locus_tag="QIE41_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HDS3531627.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLKKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLS"
BASE COUNT         5867 a         4951 c         5494 g         5863 t
ORIGIN      
        1 ataaagtcgt ccggagtatc aatatctaaa attatttctg cattttctat ttcgacggaa
       61 taatgatctc ccataaggag tgcctgacgc atattggtga tattgggacg ctggattgcc
      121 tgcatcaggc atggctttga cactaaaatc ggatggccgg gaatgccatt atggagtggc
      181 agtattgcgc catcatttcg taacgaccag atttttctaa aaatatcgat ggtgagggta
      241 ggcatgtcac cgtgggtgag aaaacaatat tctgttttta ccgcgggtac tgcggccttt
      301 actgatgtga gtaaaccctg cgcataatct gggttgtgaa taatagtgat attgctctgg
      361 ttcgcataac gttcgtgcag ttcattacca cgatagccgg tgactaaaat aattcggcta
      421 caaaactgca acgcattttt gatacttgta tcaagaattg ttccctgttg ccagggtaac
      481 atcattttcc attgccccat tcttgatgat aatccggcag cggtaattat acagtcgatg
      541 gctgacatag tggttccatt atttataggg gctaataacg cctgataatt gcgttacttc
      601 ctgatggatt aagttgggga taatttatcc cagagaggtc ataaagactc gtgaaaagta
      661 tagttgaccc atcggcatta ttcattgatt taggcgcgca gaaacgtccc actgtgattt
      721 ctgttgttgg ggctgggggg aaaaccagct tgcttttttg gctggcagaa ctgctccagg
      781 ctagcggcag gcgtgtgtta atcactacga cgacacatat gtttatgcca acgtctcact
      841 ggcccgtggt tttctgtcgt gatcccgcca tgcttcctca tgcgtctctt acatccccca
      901 tttcattttg ttttcacagc tggaaagcga accagggaaa ggtgcaggga tttacgccag
      961 aagcgattga tgcactggtg caacgaccag agtgtgacgt aattctcatt gaggcagatg
     1021 gctcgcgtgg aatgccgtta aaagcgcctg atgagcacga accttgcata cctaaaagca
     1081 gttgctgcgt gattgctgtg atgggaggac atattttggg tgcaaaagtg agcacagaaa
     1141 atgtccatcg ctggtcgcag tttgctgata ttactgggtt aacacctgat gcacccttgc
     1201 aactgagcga tctcgttgcg ctggttcgcc accctcaggg cgcgtttaaa aacgtaccgc
     1261 aaggttgtcg gcgagtctgg ttcattaacc gtttttctca atgtgagaat gcgattgcgc
     1321 aaagcgagct ccttcaaccg ctgcaacaac acaacgtaga ggcaatctgg ctgggcgata
     1381 tacaagaaca tcctgcaatc gcgcgcagat ttgtgaatta gcggcccggg tattccgggt
     1441 tactgaaagg atatccattg aggttggtat gaatattttc acagaggctg caaaactcga
     1501 agagcaaaat tgtccgtttg cgatggcgca aattgtagat agccgaggct ctactccccg
     1561 ccattctgca caaatgttag tgcgcgccga tggttctatc gtcggtacaa ttggtggagg
     1621 aatggttgag cggaaggtga ttgaagagtc gcttcaggca ttgcaggaac gtaagccgcg
     1681 attattccat ggacgtatgg ctcgtaacgg tgcggatgct gtcgggtcag attgtggagg
     1741 tgcaatgtca gtatttatca gcgtccatgg tatgcgtcca cgtctggtgt tgattggcgc
     1801 ggggcatgtc aaccgggcga tagcccagag tgcggcgcta ttaggatttg atattgccgt
     1861 tgccgatatt tatcgcgaaa gccttaatcc tgaattattc ccaccatcaa ccacgcttct
     1921 ccatgctgag tcgtttggtg cggcagtgga agcactggat attcgccctg ataattttgt
     1981 cctgattgcc acgaataatc aggatcgtga agccctcgac aaactcattg aacagcccat
     2041 tgcatggttg gggttgctgg caagtcgtcg caaggttcag cttttcctgc gtcaattgcg
     2101 tgagaaaggc gtggctgaag aacatattgc ccgtttacat gcgcctgttg gttacaacat
     2161 aggtgcggaa acgccgcagg agatcgccat cagcgtgctg gcagaaatat tacaggtgaa
     2221 aaataacgcg ccgggtgggc tgatgatgaa accttctcat ccttccggac accagctggt
     2281 ggtgattcgc ggtgcggggg atatcgccag tggtgtggcg ctacgtctgt atcatgcggg
     2341 ttttaaagtg atcatgctgg aagtggaaaa accgacagtg attcgttgta ccgtggcgtt
     2401 tgcccaggcc gtgttcgatg gcgaaatgac ggtcgaaggc gtcactgccc gcctggcaac
     2461 cagctctgcg gaagcgatga aacttaccga acgcggattc atccctgtga tggtagatcc
     2521 cgcctgttca ttgcttgatg aactgaaacc gctttgcgtg gtggacgcta ttctggcgaa
     2581 acagaatttg gggacgcggg cagatatggc accagtaaca atcgcgcttg ggccgggctt
     2641 tattgcaggg aaggattgtc atgcggtaat tgaaacaaat cgcgggcact ggctcggtca
     2701 ggtgatttac tctggttgtg cgcaggagaa taccggtgtt cctggcaata ttatggggca
     2761 taccacccga cgggtgatcc gtgctcctgc tgcaggcatt atgcgatcca acgtgaaatt
     2821 aggcgatctg gtgaaagagg gcgatgtgat tgcctggatt ggtgagcatg aaattaaagc
     2881 accgttgacg gggatggtgc gtggcttgtt gaacgacgga ctagcagtgg tcggtggttt
     2941 taaaattggt gatatcgatc ctcgtggtga aacggctgat ttcaccagcg tttctgataa
     3001 agcccgggcg attggcggcg gcgtacttga ggcgttaatg atgttgatgc atcagggtgt
     3061 taaagcgacg aaagaagtgc tggaagtggc ttaaatgaaa atggccggat ggtattaccc
     3121 acccggcctt taaattactg acaaatcacc gttccggttt tgccttcaat cccttctttc
     3181 gctttgctca atacggtaat cagtgcttca cgacctgcgc gagagcgagc aaacgaggcg
     3241 gcagcttcca ctttaggcag catagaacct ttcgcaaaat gaccttcttc gataaagcgt
     3301 tcagcatcac ttaatgacag gcgatcgagc cactgttcat tctctttacc gaagttaatg
     3361 gccacttttt ctaccgcagt cagaatgatc agcatatcgg catcaatcat ttctgctaaa
     3421 cgggcgctgg cccagtcttt atcgataacc gcactggcac cacgcaggtg gttaccttca
     3481 cgaataacag ggataccacc accgccaacg gtgatcacca cctggcctgc atctaccaac
     3541 gctttaaccg tttctttttc aatgatatca accggttttg gcgaggcaac aacacggcga
     3601 tagccgcgac cagcatcttc tttcagagtg taaccctgtt ttgtcagttg ctctgcttct
     3661 tgctcggtaa agaacgaacc gatcggcttg gtcgggttga ggaatgccgg atcgttagcg
     3721 tcgacttcga cctgggtaac cagcgttgct acaggtttat taatgccacg agaaagcagt
     3781 tcttctctta aggcattttg tagatcgtaa ccaatataac cctggcttaa ggcaacacag
     3841 acagacatcg gcagcattgg cgagtgcgct tcagtttttg cggcagcttc aaaggcctgg
     3901 ttaatcatgc ccacctgcgg cccgttacca tgagtgacga tgacctcatg tccctgggca
     3961 attaaatcaa caattgcctg agaagtaatt tttaccgctt tcatttgtcc ggccaggtca
     4021 tcgcccagcg cattcccgcc cagggcgaga acaattttct tactcatttt tcctcactca
     4081 tcattcttga ttgtgtatag atccctggca gtggttagag cacgggaggg acaaacggtt
     4141 tgcgtcgcag gaatcgacct cgcccggctt tgcctgtaaa ggtgccgtca cagaaaatcg
     4201 tttcaccacg ggataaggtt ctgacaatcg cgccctgaca ggtaaaaccc tcccatggcg
     4261 agtagtcggc gttgtcgtgg agatggcgat gctgaatttg ttggctctga cgtgggtcga
     4321 taatcaccac gtcgccatcg gaaccgggcg ctaataatcc tttttgcggc cacaggccaa
     4381 acagcctggc gggcattgcg ctggttaatt caacaaagcg ttccggtgtt atacgtcccg
     4441 tcatcacgcc actggaaaac agtaactgca tgcggttttc cacaccgggt aagccatttg
     4501 ggcagcgact gaaatcgcct ttagaaattt gcaggcgttg agccatcgag aaggtgcagt
     4561 gatcggttgc caccacgtca atcgcaccat cgctgatgcc acaccacagt ttgtcctgct
     4621 cgcgtatgtt acgcagcggt gggctaagaa tgaacttcat gccatcttct gtatcgtaac
     4681 tgcgttcgtc caacaggaga tattgtgggc aggtttcaac ccagactggc tggtgattcg
     4741 cacgggcaag acgcagataa tccagaccta agccgttaga caggtggacg atatagagcg
     4801 gggcgttacc ggcaatttgt gccaggttaa tcatgcgggc gatggcttcc gcttcgcatt
     4861 ccagagggcg actcaaggcg tgatagcgcg gcgcggttaa ccctgcggcg ataaattccg
     4921 cccgcttgct ggcgatagcc gcatcatttt ccgggtgcac ggtggtcagc gcgccggact
     4981 catgcaagcg gcgtaacgcc tgcaaaacct cgtcatcgtt gagtttgtat tgataggtta
     5041 aatagagttt aaaactgctc agtccttctt cgaccatcat cggaatttcg tcgaggatgg
     5101 cgtgattaat gtgctggatc acaccgtgaa agctgtagtc gatgaccgcc ttatgggcgg
     5161 catagccacg ataaacttcc agttgatggc gtaatcggca gccattcggg ccaaatccca
     5221 tatggtcaat aatggttgtt gtaccgccac acgcagctgc gcgggtaccg gtaaaaaaat
     5281 catcacaact gcgcgcgatg ccgacatcaa tattgaaatg cgtatggaca tccacgccgc
     5341 cagggaaaac gtaacagcca gtggcatcaa tttcttcata cgggagctgc ggcgaaatat
     5401 tgctgcccaa ttggcgaaca atcccgctct caatcagcaa atcttgtttg gcttgtccat
     5461 ctgcgttaac gacagtgccg tttttgatca atacgcgcat agcaaactcc aaaaaccctc
     5521 cggcgaaccg gaggggaata agtgaagagt aattattcgg ttgccagcca gcttaacggg
     5581 attgcagcgt acatcgcggc acaggtcacc aggtgagatt tccaggtttt ttcgttaggt
     5641 gcatgcgctt caggttcttt acccgggcca aagccgatca ccggaatgcc gtgacggccc
     5701 atgatagata cgccgttagt tgagaaggtc cacttatcaa caaccggcgc tttgccaaac
     5761 agaccttcgt aggcattcac cagtgctttg acagtgaagt ggtcttcttc cactttccag
     5821 gtcgggaagt agcattcggt tgggtaaacc aggccagtcc aggacggacg gtcgtagttg
     5881 tacatagaaa caaccgcgtt agctttctgt accgcaggca gggcgcggat ttcgtccagc
     5941 gcgccttccc aggtttcgcc ccaggtcaga cgacggtcga ttgaaactgc gcagctatct
     6001 gctacagcgc aacggcttgg ggatgtgaag aagatttcag aaacggtgag ggtgcctttg
     6061 ccgaggaatt catcatagcc caggcgctgg gagagttctt gtaattcgcc aagaatcgga
     6121 cccattttga aaatggcgtt gtcaccgcgt tctggtgcag aaccgtggca gctaacacct
     6181 tgaacatcaa tgcgaatttc catacgaccg cgttgaccac ggtatacctg gcaatcagtc
     6241 ggttcagtac tgaccacaaa ttccgggcga atgccggatt gttcaataat gtactgccag
     6301 cacagaccgt cgcagtcttc ttcctgcaca gtaccggtaa ccagcagggt atattcatct
     6361 tccagaccga ggtctttaat gattttaccg gcataaacca tagatgccat gccgccttcc
     6421 tggtcggaag taccgcgacc accgatcagc tcatcagttt ccatgccttc gtacggatcg
     6481 aagtcccagt ttttgatgtt gccaatgccg acggtatcga tatgagcgtc cattgccacc
     6541 agacgcgggc cgtggccgat ataaccgaga acgttgccca tcgggtcgat ttcaacttta
     6601 tcgaagccga ctttttccat ctcttcttta atacgatgta ctacgcgttt ttcgtcgcag
     6661 ctttcactgg gaatagcaac catgtcgcgc aggaagcgag tcatgtccgc ctggtaatct
     6721 tttgcttttt caagaatcag tttgaatgga atattcttag ccattatttt tctccagatt
     6781 ttttactccc cgttttgcag ggagcagttc caatagggtg attaaggtgc tacagcgtgt
     6841 ttgccttccc agacaacttc gcggtagtgc ttcacgtctg tgtcgccttc agtgctgata
     6901 accagcacta cggcatcttt gttcagcgcc agtttttcca tcaggctttg acgttgcggg
     6961 tgataatgaa ccgctgcgag aacgcccaaa ccgacagcgc cggattcacc ggagatgatg
     7021 cgtgggtcat tgccgtacgg gttacccagc acacgcatac ctaatgcggc aacgctgtcc
     7081 tggcaggaga tgaattgggt ggcgcagtta cgtaggattt cccagcccag cgggttaggt
     7141 tcgccgcagg ccaggcccgc catgatggtg gccatatcac cgccaacgtt gacgatgtcg
     7201 cctttgacgc cggagcgata aatacagtca gctttgtcag gttcaacaat aatgctgtgc
     7261 agattttgcg ggctgtagac gtcgaccaga taacccagca caccaccggc cattgcaccg
     7321 acaccggctt gcagcagaac gtgcgtcggg gttacgccca tttcacgcat ttgctcgacg
     7381 gcttcatctg ccagtgttgc gtagccttgc atgatccagg ttgggatttt ggtgtaacct
     7441 tcccaggcgg tatcttgcac cacttcccag ccgtgctgct gcgcgtgttg catggtcagg
     7501 cgaacggtat cgtcatagtt catatccgtg acgatgcact cggcaccgag gttcagaatg
     7561 gcgtcaacgc gttcctgagc agaacctttc ggcatgtaaa tcaccgcatt ctgtccgagt
     7621 tgctgtgctg cccacgccac accgcgcccg tggttgccgt cggtggtggt cgcgaaagtc
     7681 attttttcgc cgatggcatt tttcaggtgc tcaaatgaca gcgtttcgat atcaagatga
     7741 tatttttcgc acaataattg agcgatggcg tatgcaccgc cgagcatttt gaaggcgttc
     7801 agaccgaatc gttttgattc gtctttgacg agaattttct tcacaccaaa aaggtttgcg
     7861 agatcgttca gcgcacaaag cggtgttggg cgataaccag caattttctg gtggaactgg
     7921 cgcgccagtt tggcctggct ttgcgaaaag agcggtgatg tttcgccgtt gaaaaatttg
     7981 ttatcggcga tatcaatctt caatgagaaa acggacatat cctatccttt tggcctctgg
     8041 aaatagaggg ccgaccagct gatcggcccg aggggttatt tcacgcgttc ttgcgcttct
     8101 ttcagcagtt gctcgagcag tgcacctggt ttggcgtatt tacgggacag gatcatcgcg
     8161 gcgatgatat aaggcttcca gctggcttct ttgtaggtcg cgatacggta tttttcgaat
     8221 acgccttcag tcacttcacc ttctttgcag gatacgccgc tgatatcagc tggcaggcag
     8281 tgcatgtaca gggcttcgcc atcacgggtc agttccatca tctcttcagt acaatgccag
     8341 tctttgtgtt gcgcgttctg tgccagacac tgtttttcca gtgcttttaa gccttcgtga
     8401 tcgttcgcac gcagcaattc agtacgctct tccatcactt tgtaaggtgc ccatgacttc
     8461 ggataaacga tgtctgcgtc tttgaaggct tcttccatgc tggtgacctg acggaagcta
     8521 ccgccggagg ctttagcatt gtttttcgcc acttcaacga catccgggat caggtcgtag
     8581 ccttccggat gggccagggt gacatccata ccgaagcgag tcatcagacc gatgatgcct
     8641 tgtggtacag agagcggttt gccatagctt ggggagtagg cccaggtcat ggcgattttt
     8701 ttacctttca ggttttccag tgaaccaaag tgttcacgta accatgccag gtcagccatt
     8761 gactgagtcg ggtggtcaat atcgcattgc aggttcacta aagccggacg ctgcggcagt
     8821 acgccctgct tgtagccgtc atcaagtgca gcgccaactt cacgcatata agcgttgcct
     8881 gcgcccagat acatatcgtc gcgaatacca atagcgtcgg cgcagaagga gatcatattg
     8941 gcggtttcac gcacggtttc gccgtgagcg atttgtgatt tgccttcatc gagatcttgc
     9001 tgtgcaaggc cgagcaggtt aagcgcggaa gcataagaga agcgggtacg agtggagttg
     9061 tcgcggaata cggaaatacc taatccacta ttaaagactt tggttgagat gttttcagca
     9121 cgcagtgctt ttaatgctgc ggcaacgtcc agtacttgtt tcagttcatc tggcgtctgt
     9181 tcccacgtta acaaaaaatc tttctcgtga aggtgagagg tcagcgaatt gatatcctta
     9241 atcagctcat taacagtttt catcataaag tcccggtagt ggaatgacag gcaaagtggt
     9301 ttatttcact gtgacttgcc aaagtgattg gtgttttcac agctatacct gggactaaca
     9361 ataagtgtgc cagaattatc cacggggctg caaaagacta atttgacgga taatgtgtgg
     9421 ggttgatcac aaattgatac cagcttctgg ttgtttgtaa aatacgaaaa cgtttggctt
     9481 tttttatcgc gttatttatt tgctgctaag tatcagaaat aagataaagg ggcaggttat
     9541 ccctcgtcag tttgataaac tcaccgccgt aattctcata ttgatataga aatgtgatat
     9601 ctcttccata atttcattag aaagtgataa aaatcttatg tttttatcaa agccaatttc
     9661 gtctgagtta atatcatcat gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa
     9721 gtaagtctgg aaggtaaagc tatggagctt gctactacgc agtcagtact gatgcaaatt
     9781 caaccgacaa ttcagcgttt tgccagaatg cttgccagcg ttttgcagct tgaggttgag
     9841 atcgttgatg aaaacttgtg tcgcgtcgcc ggaacgggcg cgtatgggaa gtttcttggc
     9901 cgccagttga gcggcaactc acgcctgctc cgccacgtcc tggaaacgaa aactgaaaaa
     9961 gttgtgacac agtctcgctt cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc
    10021 cgtgaaaaag catttctggg tacgcctgtc attttacagg atcgttgtgt tggggtgata
    10081 agtttgattg ccgttaccca cgagcaacaa gaacatatca gtgataattt acgcgaattt
    10141 tctgattacg ttcgccatat atccaccatt tttgtttcga aacttctgga ggatcagggg
    10201 ccaggagata acatcagtaa aatattcgcg accatgatcg ataatatgga tcagggcgta
    10261 ttagttgttg atgatgaaag tcgggttcag tttgttaatc agactgcctt aaaaacactt
    10321 ggtgttgtac aaaataatat tattgggaaa cctatccgtt tcagaccatt aacatttgag
    10381 agtaatttta cccatggaca tatgcagcat attgtttcgt gggacgataa aagtgaatta
    10441 atcattggtc aattgcataa cattcagggc cgacaattat ttttaatggc gtttcaccaa
    10501 tcgcatacca gtttttctgt agcaaatgca cctgatgaac cacatattga acaattggtt
    10561 ggcgagtgcc gtgttatgcg gcaattaaaa cgactcatta gccgtattgc acccagccca
    10621 tccagcgtta tggtggttgg tgaaagcggc acgggtaaag aagttgtcgc ccgtgcaatc
    10681 cataagttga gcggaagacg gaataaaccc tttattgcta tcaactgtgc cgcgattccg
    10741 gagcagcttc tggagagcga actgttcggt tatgttaaag gcgcatttac tggcgcttct
    10801 gccaacggta aaacagggtt gattcaggcg gcgaatacgg gcacgctgtt tctcgatgaa
    10861 ataggtgata tgccattaat gttgcaggct aaattactgc gcgctattga ggcgcgtgaa
    10921 attctgccga ttggtgccag tagcccaata caagtcgaca tccgcatcat ttctgcaact
    10981 aatcagaatt tggcccagtt cattgccgaa ggtaaattcc gcgaagatct cttctaccga
    11041 cttaatgtta tctcgataac tcttccaccg ctgcgtgaac gccaggaaga tattgaacta
    11101 ctggtgcatt actttttaca tctgcatacc cgtcgtctgg gatcggttta tcctggcatt
    11161 gctcctgatg tcgtcgaaat attgcgtaag catcgttggc ccggaaacct gcgcgagtta
    11221 agcaatttga tggaatatct ggttaacgtt gttccttcag gtgaagttat cgatagcacg
    11281 ctattgccgc caaatctgct gaataatggc acaacggaac aaagtgatgt aacagaggtc
    11341 agcgaggcgc acctggcact cgatgatgcg ggcggcacgg cgctggagga gatggaaaag
    11401 caaatgatcc gcgaggcgct ttcacgccat aacagcaaga agcaagttgc tgatgaactg
    11461 ggcatcggta ttgctacgct ctatcgcaag attaagaaat atgagttgtt aaacacataa
    11521 cctcagtgaa tccggcctga attcaggccg gatatccttt tacttcgttt tctcgcaatc
    11581 cagaactgta tttacaatca tctgataccc cgtgcagcga caaagatttc ccgccagacc
    11641 gcgacgaatt tccgtaatgg ttaatggctt ctcgcgcggc tttgccagca ttgccgtggt
    11701 agccataatc aggccaggcg tacaaaaccc gcactgcacc gcgccggatt tcgcataagc
    11761 ctgctgaaca tgagaaagtt ttccgccttt cgcttcacct tccagcgtgc ggatctcttt
    11821 tccttcagcc caggcggcaa ggtataagca actgtctatt gctgtgccgt cgaccagcac
    11881 cgtacaggca ccacattcac ccacgcagca cccttgtttg acacttagca gtccttgatc
    11941 gcggagtaat tccgagagcg gcatgcctgg tgcggcgtga agctgaaaag gcatcccgtt
    12001 aatggtgcat tcgatggtaa ttgtttcact gtgattcatt gcaattttcc ccccgccgcg
    12061 gcgacggctt cgctaatcac ttttttggtc attgtctgga tgagatgcag acgaaactct
    12121 ttactggccc gccatgaaga acgcggggcg acatcttgca ggacagattc gctgatagct
    12181 tccagcgttt gcaggtttaa tggcgcattt tgtgcagtct gttcggcatg ttggcagcga
    12241 atcggcgttg gcgcggcaac accaaatgcc aggcgtaatt cgctgaaatt gccgctatcc
    12301 agtcggcaat gtgcggcaca gccaatcgtt gaaatatcca ttgcgtcgcg catggcatat
    12361 ttaaaatgcg cgctgcccac gtgttctttc ggctggggcg gaaaatgaaa ggcgacaagg
    12421 atttcgtcat gctcaagaga cactttgccc ggcccggtgt gaaagccatt aatcgggacg
    12481 aaacgaacac tgcgtgggga gtggatctcc agtttcgcgt cataaatgag cgttggcgtg
    12541 gcagaatctg cgctggtggc accgttgcaa atatttccac cgtaggtagc gacgttacgg
    12601 atctgcggtc cagcaatgga cgaggccgca gcacataacg ccgggagaag acgttgagtt
    12661 ataggatctt ctattagctg ggtaaatgtc gttgcagagc cgatacgtag tgagccatct
    12721 tccgccagcg taattccccg cagctccgcc agattatgaa tatcaacaat atggcgataa
    12781 cggtcattgt gatggtggag ctgaatcagt acgtcagtgc caccggcgag cagtttggcc
    12841 tgcgggttgt cagccagcag gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa
    12901 gcaaaatcaa acatgatgtt atcctcaaat caatcctgcc agatggaact cttcatataa
    12961 ccgttttggc gtcagcggca gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt
    13021 acgaatagca gcggcaacag gaattattgg tggctcacct agtgacttat gtccgtatgc
    13081 ggattgcggc tcattgattt cgatgaacgc gctttccagt tgtggcagat ccggcatggt
    13141 gggcattttg taatccagca gattggggtt acggaccacg ccgcttttcg catcgatgat
    13201 catctcttca aatagcgccc agccaatgcc cattcccatt ccgccgtgta cctgaccttc
    13261 tgccagcaac ggattaagaa tatggcccga atcatgaacg ttgaggatgc ggttgatggt
    13321 gactttgcac agcgcaatat cgatcgtcag atcaacaaag gtacagccaa acgctggtgg
    13381 gttagtggtg gttttgatgg agctttcggc agagagctgc ccaccgcgtt cagggtggta
    13441 gaaagcgtcc atcgccaaat cttttaacga cattaacggc tcttccggtc gttcaaccag
    13501 cacgatatgg ccttttatca gggtcagatt catcgctgac tgatgtagca tgactgcggc
    13561 gtgagcgatg attttctctt ttaataacag tgccgcactg cgcagcgcag gcgcggcaac
    13621 atagctctgg cgtgaggcaa atgcgccggg atcgaacggc gtaacatcgg tatcttgtgt
    13681 tgaaataacg cgaacgtcgc tgaccggaac ccccacggtt tctgccacca tttgcgagaa
    13741 gacggtgtcg gcaccctgac cgatttccgt cgcgccgctt tgcacgttga tggttccatc
    13801 ctgattcatc agaaggcgcg cgcctgctat ttctacgccg acaggccagg tgttcgaggt
    13861 gtagctaaaa caggcgacgc caacgccgcg gcgtaaattg ccttgctggt tctggcactc
    13921 tgcacggcgt ttttcccatt caaagatttt ccggcctttt tcaagacact ccggcaaccc
    13981 tgcgctgtaa atacgtttgc ccgtgagcgg attagcatct ccttcgcggg cggcgttgcg
    14041 taaacgaatt tcaacaggat caatacctaa cgctgtcgcg gcgtcatcaa gcatagactc
    14101 aacggcaaat acgacttgtg gcgcgccata accacgcatc gcaccagccg aggggaggtt
    14161 ggtatagcag gtctttgaac tgtaagcgta ggcacaacga ggataaaggt aagcgacttt
    14221 attcccccct gcagaagcga tggagtgccc gtgagatgca taagcgccgg tgttagacag
    14281 aacatccaga ctataacctt tcaatgttcc gtcgcggttc acgcccattt gcccgtcaat
    14341 agtaaaagcg tggcgggtac gggttgcgag gaaacactct tcacggctaa gggaaacttt
    14401 caccggaatg ccgccaagct tgctggtcag gaatgctgcc attggctctt ccagtacatc
    14461 ctgtttatta ccaaaaccgc caccgacaaa cggtttgatg actcgtacgc atgaccaggg
    14521 gatatccagc gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg tgctggaaac
    14581 gatggtaatt cgcgagtcat cctccatcca tgccagcgat gtcacgcttt ccatgtgaca
    14641 atgttgaatg accggcgtct gatagtgccc ctgtacctgg tagtcggcgg catcgattgt
    14701 ttgttggaca ttgcccgtcg tcatcgtgct ttgttttagt aaattgccgc cgttatggat
    14761 tggttcagcg tcatcagcca gcgccgcttc tggggtggtg ataacgggta attcttgcca
    14821 ctcaatgctg accaattgcg ccgctttttc tgccgtgagt tcatcacggg ccacgacgat
    14881 ggcaacggcg tcaccatggt gacgaacatg gcgagttagc agtgcgcgat cggcggtatc
    14941 gcgcttgttt tcgtcaagtg tccaggcatg cccagctgta gcgaatggga tatcaggcac
    15001 atcttcccag gtaaaaatcg ccagtacgcc cggtaaactt ctggcttgtt catcattaat
    15061 acttacggca taaccatgtg cgatagggct acgtacatat ttcgcgtaac acatgcccgc
    15121 cataacataa tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg catctacgcg
    15181 catgcatgat tcacctgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg
    15241 aacatttggt tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacga
    15301 cgataagcgt taagggagga aggaagtgtg atgatgttaa aaaaacacag atatgatacg
    15361 aaacaggacc ctgaataggg gcagtgatac gtatcaatat gagtggcgat aaaatcaatg
    15421 tgataaattg ggattattac gcatcaggat gggaaatagg gtaaatgagt caggaaatta
    15481 gatacatcta cagtgaatat tttgctgatg atacttccct ttgccaaatg aaattaacct
    15541 ctttctctat taccgcgttt ttccagaagc aagagattgg gtatcaagag gctggctgct
    15601 atgatatggc gtcttgtttt ttaagcgagg aaagattttg agtgcgggac gcctgaataa
    15661 aaaatctctg ggtatcgtga tgttgttatc ggttggactg cttttggcgg gctgctcggg
    15721 tagcaaatca tccgatacag gaacgtattc cggctccgtt tacaccgtga aacgggggga
    15781 tacgctatat cgtatttcgc gcaccacggg aaccagcgta aaagagctgg cgcgactgaa
    15841 cggcatttcc cccccttaca ccattgaagt tggtcagaaa ctaaaactgg gtggggcgaa
    15901 aagtagcagt acacgtaaat caaccgccaa atcaacgacc aaaaccgcat cggttacacc
    15961 gtcatcagca gtaccgaaat cttcctggcc gccagtaggg caacgttgtt ggttatggcc
    16021 aacgacaggg aaagttatca tgccgtattc gacagctgat ggcggcaata aagggattga
    16081 tatctcagct ccacggggta cacctattta cgccgcgggt gcaggaaagg tggtgtatgt
    16141 gggcaaccag ctgcgtggct acggtaatct catcatgatt aaacacagtg aagattacat
    16201 tacggcttac gcccataatg acacgatgct ggtaaataat gggcaaagcg taaaggctgg
    16261 gcaaaaaatc gccactatgg ggagcactga tgcggcatcg gttcgcctgc atttccagat
    16321 tcgttaccgt gcaacggcaa ttgatccgct acgttacttg ccgcctcagg gcagcaagcc
    16381 aaaatgctga tggcgaatta atcagcagtc agcagcgcgg cacttgctaa ggagagcgta
    16441 aggtttataa tgccttacgc atctcgaagc gggcgtagtt caatggtaga acgagagctt
    16501 cccaagctct atacgagggt tcgattccct tcgcccgctc caatttatct tcacctcatc
    16561 aacctgccat ttttgttgta tctgattttc tatattatct taattcaatc gcttcagact
    16621 tttcgcaatc gccgtaatac ttccccattt ttttgtaaat aaaagcgttt acgcaatgca
    16681 atccatttgt aacaacccag gtaaaatacc ttattttcaa gttgttaaat aagtggttat
    16741 ctacgctttt aacaaacgat tgatggaatc ttttatgaaa gggaaatcta cattaactct
    16801 tctgctcgct ggtatatttt catgcggcac atgccaggcg acaggggcag aagtgacgag
    16861 tgaatctgta tttaacattc ttaattccac gggggcagca actgataaaa gttatttatc
    16921 gttaaatcca gataagtatc ctaattatcg tcttctgatt cactcagcca aattaaaaaa
    16981 tgaaataaag agccactata ccaaagacga aattcaagga ttgctaacat taacagaaaa
    17041 tacacgaaag ttaacgttga cggagaaacc ctggggaaca tttattcttg cttctacttt
    17101 cgaagatgac aaaacagcgg cagaaaccca ctatgatgct gtctggctga gggatagttt
    17161 atggggctat atggcattag tatcagatca gggaaatagt gtagccgcaa aaaaggttct
    17221 gctcactctg tgggattata tgtctacgcc cgaccagatt aagcggatgc aggacgtcat
    17281 tagcaaccca aaacgactgg atggtattcc tgggcaaatg aatgctgtac atatccgatt
    17341 tgacagcaac tcccccgtga tggctgatgt acaggaagaa ggcaagccgc agctgtggaa
    17401 ccataaacaa aatgatgctt taggtcttta tcttgatctc cttatacaag ccatcgacac
    17461 tggtactatt aacgctgaag actgggaaaa aggtgaccga ttaaaatcga ttgcgctgtt
    17521 ggttgcttat ctggataaag ccaatttcta tattatggaa gattctggag cgtgggaaga
    17581 agatgcccga ttaaacacat cttccgttgc actggtgacc tctggactgg aacgtttgtc
    17641 gaacttactc tcgaagaaag actctgtttt tatctctgat ttactgcgcg aagctaaagc
    17701 caatgaactg gatgaacctc tttcaacaac gaggttgaac cacctcatag ataaaggtta
    17761 tgaaagaata acgctacaac ttgatttagg gggagaatcc cctggttatc tggaaaaaga
    17821 taaacattac agagaagctg atgcggcctt acttaatgtt atttatcctg caaatctttc
    17881 aaagataaac accagaagaa aagagcaagt tctaaaaata gttaaaaaat tagcaggtcc
    17941 gtatggtata aaaagatacg agaaggataa ttatcagtcg gcaaattttt ggttcaatga
    18001 tattaaaact gataccgatc aaaattcaca tgcgaaaaga gaaaagagct tcattccatc
    18061 aacagaagca gaatggtttt ttgactcatg gtatgcaaaa tcagcagcaa ttgtttataa
    18121 agaatcaaga aaagaagaat atttgaatga ctcagtgcaa tttatgaata gatcattggc
    18181 ccaaataaca ggcgagaata tgattggtgc aaatggcagg agcgtacctg aaatggcact
    18241 tcccgaaagc tataactata tccacaagtc aggaactttg catgaagcgc caagccctat
    18301 cattccgcta aactggtcga aagcctcaat gactcttatg ttgaaagaaa tgtccaacct
    18361 tatcatgatg agggaaataa atagttttca taatgaggcg gtcctggatt aaaaggaccg
    18421 ctcaaagaga aatcgtaagg gaggtaaaag tgatcaataa attactgtta gcttacctga
    18481 taggtctggt tgtgacatcg accttaatat ttatcttctc ggaagaaaaa gtaacatatc
    18541 ggttgtttgc tgcagtaatt acagggttga cctggccgct tagcctcatt ccatccatca
    18601 taagtctcat gattcgtaaa tccgactaac aagaaaatta tatgaacgat taagaaaaat
    18661 agaatcgaaa aagagaataa aatatatgcg gggagtaaca aaacactcgc agtacgttaa
    18721 tgtgtaacta acaaatatga tttttattga aaatagtcac caaacaagtg ttataagaaa
    18781 tacggggtat gtttaagtaa aacatatcaa cagttgcaat tctgtatatg gacagaatga
    18841 cttactactg taagtggctg atagctattc gctcataaca aaatcattat tttataaata
    18901 ggaatcatca ctttttaatc atgtttttat tgcaagttta tggagaatga gaatatggaa
    18961 taaggagatg aaacatgatt gaagaagggc tgttacttcc aatgaatgtt ttgcttcaag
    19021 gacagaagtt aaccctactg gaccctgaaa tgtggttctt acggggaaat gaaaacaagg
    19081 atgtaaccct tgttattacg attggtaata accatcaaaa attaatggta gtggaagaca
    19141 tattactgct cattgaccga agccaaattg aggtaactgc agggaaagta atttatcatc
    19201 cacttcgcat tgatatcctg agtaaattat tagcttttat tgatgagtca acggggagtg
    19261 tggaaaggga acgccatgag ttgtttgctg atgccttgcc ctttgcatca gaagttgtgc
    19321 tattcagtaa agttgcaagt gaagcctggt ttttagccac ttatctgagc agcgataata
    19381 ttaatgaaat tctgtttcag catctaagaa aaacagaatg ctataaactg gttcgctatt
    19441 tattatcgca atcgctcata cagacttcgc tctatgatct tggtgagctt tacggcgtat
    19501 cgtattcaca ttttcgccgt ttatgtagtt atgcattggg tggtaaagtt aaaacggaat
    19561 tatgtggctg gagagttgcc agagccgtgt tggaaatcat tgaaggaaac agtgatatga
    19621 caactattgc gcataaatat ggctactcat cctcatcaca cttttcagcc gaagtaaaaa
    19681 gccggttagg aaaaacaccg agagaactat gtaaaaagtt atgaaaataa aattacgtat
    19741 tactattata ttaatttcag ccttatgcat ttttaatgga ttattgactc ctggtgcata
    19801 tgccgcaaca gcgaatggat acgtagcaaa taaagaaaat ctacgtagtt tttttgaaac
    19861 ggtttcatca tatgcgggta agcctacaat agtaagtaag cttgccatga agaaacagat
    19921 cagcggaaat tttgatttaa ccgaacccta tgccttgatt gaacgcctgt cggcgcagat
    19981 gggacttatt tggtacgatg atggcaaagc tatttatatc tatgactcat cagagatgcg
    20041 taatgcgctg attaatctga gaaaggtatc gacgaatgag tttaataatt ttttaaaaaa
    20101 atcgggtctg tataactctc gttatgaaat taaagggggc ggcaatggta ctttctatgt
    20161 ttcagggcca ccggtgtatg ttgacctggt cgttaatgct gcaaaattaa tggagcaaaa
    20221 cagtgatggc atcgagatcg gacgcaataa agtagggatt attcacctgg tcaacacatt
    20281 cgttaatgat cggacttatg aattacgtgg cgaaaagata gttattcctg gcatggcaaa
    20341 agtactatcg acgttgttaa ataataacat taagcaaagc actggggtta acgtactttc
    20401 tgaaatttca agtcgtcaac aactaaaaaa cgtatcacgt atgcctcctt ttcctggtgc
    20461 tgaagaagat gatgatctac aagtagagaa aataatatcg acagctggag cgccagagac
    20521 tgacgatata caaattattg catatcctga taccaacagt ttacttgtta aaggaacagt
    20581 atctcaggtt gattttatcg agaagttagt agctacactc gatattccaa agcgtcacat
    20641 tgaattatct ttatggataa tagatattga taagactgat ctagaacagt taggagctga
    20701 ctggtcgggc acgataaaaa ttggatccag cttaagtgca tcatttaata attcaggttc
    20761 aattagtacc ctggatggga cacagtttat tgcaacaata caggcacttg cgcaaaaaag
    20821 aagagccgcc gttgttgctc gtcctgttgt tttaacacag gagaatattc ctgctatatt
    20881 tgataataac cgtactttct atactaaatt agtcggcgag cgtactgcgg agttagatga
    20941 agtaacctat ggtacgatga ttagcgtgct accacgattt gcttcgcgaa atcaaattga
    21001 attactcttg aatattgaag atggcaatga aatcaattct gacaagacca atgttgatga
    21061 tctgcctcag gttggtagaa cgcttataag tactattgcg cgcgtgcctc aagggaaaag
    21121 tcttttgatt ggtggatata cacgagatac gaatacctat gaaagtcgca agatcccaat
    21181 attaggcagt attccattta ttggtaaatt atttggatat gaaggaacaa atgcgaataa
    21241 catcgttcgt gtctttctta ttgagccaag agagatcgat gagcgcatga tgaataatgc
    21301 gaatgaggct gcggttgacg ccagagcaat aacacagcaa atggcaaaaa ataaagaaat
    21361 caacgatgaa ttactgcaga aatggataaa aacttacctg aatcgtgaag tcgtcggagg
    21421 atagtgaaat ggcgattcat gtagaacatg tcggtgtatt agaaagagcc agagaagttt
    21481 ctcgcttaga ggacattatc acagaagata atgaagatat tgaagctgaa atgcctaaaa
    21541 tgggagatga tcccgccggt aaagaagctc gattcttaca agctactgac gagatgtcag
    21601 cagcgttaac gcagtttatg aagaaaaaaa tctacgaaga acaactggcc aactttctgg
    21661 acggagaaga atatgtcctg gaagatcagc caatagaaaa aaccgataaa gtcatggagg
    21721 cgcttaaggc agcaactacc catgactatg aagtgtatag ctttgctaaa aaattatttc
    21781 cagatgaaag cgatctggtg gttgtattac gtgcaatttt acgtaaaaaa caaatatctg
    21841 aaaatgtgcg gctaaatgct gaagcattac ttaaaaaagt taatcaagag actactaaaa
    21901 aattcattaa tagcggtatt aattcggcat taaaagcaaa gttatttggc caggcgttgt
    21961 cattaaatcc aaaattacta agggcaagtt atcgacaatt tttaatggca gaagacgatg
    22021 ctgtagatac ttatgtcgaa tggattggta gttatggcta tcaaaataga atgctggtga
    22081 caaaatttat caaagagacg cttttctcag atataaatgc acttgatgcg agctgttcat
    22141 cattggaatt tggtatgttt ctcaataagt tgtca
//
LOCUS       DAOTRK010000076        21912 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.076, whole genome shotgun sequence.
ACCESSION   DAOTRK010000076 DAOTRK010000000
VERSION     DAOTRK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.076"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            454..954
                     /gene="tssB"
                     /locus_tag="QIE41_002754"
     CDS             454..954
                     /gene="tssB"
                     /locus_tag="QIE41_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDS3531628.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKPLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            989..1213
                     /locus_tag="QIE41_002755"
     CDS             989..1213
                     /locus_tag="QIE41_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531629.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPYWRRVLH"
     gene            1264..2739
                     /gene="tssC"
                     /locus_tag="QIE41_002756"
     CDS             1264..2739
                     /gene="tssC"
                     /locus_tag="QIE41_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDS3531630.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2746..3159
                     /gene="tssE"
                     /locus_tag="QIE41_002757"
     CDS             2746..3159
                     /gene="tssE"
                     /locus_tag="QIE41_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDS3531631.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIAQCIERYEPRISRAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            3163..5013
                     /gene="tssF"
                     /locus_tag="QIE41_002758"
     CDS             3163..5013
                     /gene="tssF"
                     /locus_tag="QIE41_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDS3531632.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAESFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4977..6059
                     /gene="tssG"
                     /locus_tag="QIE41_002759"
     CDS             4977..6059
                     /gene="tssG"
                     /locus_tag="QIE41_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDS3531633.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAQGEKGTPELSLRDDPAQETLRFSADASLAFPLSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKKGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            6084..7364
                     /gene="tagH"
                     /locus_tag="QIE41_002760"
     CDS             6084..7364
                     /gene="tagH"
                     /locus_tag="QIE41_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HDS3531634.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDFPVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQIYDRVLREKQREPEA"
     gene            7361..7885
                     /gene="tssJ"
                     /locus_tag="QIE41_002761"
     CDS             7361..7885
                     /gene="tssJ"
                     /locus_tag="QIE41_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDS3531635.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7888..9219
                     /gene="tssK"
                     /locus_tag="QIE41_002762"
     CDS             7888..9219
                     /gene="tssK"
                     /locus_tag="QIE41_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDS3531636.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9224..9985
                     /gene="icmH"
                     /locus_tag="QIE41_002763"
     CDS             9224..9985
                     /gene="icmH"
                     /locus_tag="QIE41_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HDS3531637.1"
                     /translation="MDEGSLSLPPFTGHHEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9994..12807
                     /gene="tssH"
                     /locus_tag="QIE41_002764"
     CDS             9994..12807
                     /gene="tssH"
                     /locus_tag="QIE41_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDS3531638.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKVAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTNTSRMTEVLVQYDETLTALDELEAAWHQQQALVREIIALRQQLLGVAEDD
                     AASLPDADAAEDTPPESESESESESESESESESESEQDNTGAVPADEADREQPEETAE
                     TVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVIT
                     DLPKLLGDTIKGQDLAIASLHKHLLTARADLRLPGRPLGAFLLAGPSGVGKTETVLQL
                     AELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLL
                     DEVEKAHPDVLNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPET
                     MQEELYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDKVLRSRFGAEVV
                     IEPEVTDEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVD
                     DAFTADMEDAQNDESVTKDETVL"
     gene            12804..13547
                     /gene="tagO"
                     /locus_tag="QIE41_002765"
     CDS             12804..13547
                     /gene="tagO"
                     /locus_tag="QIE41_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HDS3531639.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTT
                     PPDATATSQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     LEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13552..14964
                     /gene="tssA"
                     /locus_tag="QIE41_002766"
     CDS             13552..14964
                     /gene="tssA"
                     /locus_tag="QIE41_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDS3531640.1"
                     /translation="MAMDLRDPNVWITHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            15073..18507
                     /gene="tssM"
                     /locus_tag="QIE41_002767"
     CDS             15073..18507
                     /gene="tssM"
                     /locus_tag="QIE41_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HDS3531641.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQSLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLAEMGLYQGRR
                     IGPYVERTYLQLLEQRYLPSLFNGLVKEMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGGQPLQRALTVLRDNTQPGAFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLTVQKDKESTMQAVYLQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMSKTLPAPLNRWAGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPHSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLVGARGGAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18518..19870
                     /locus_tag="QIE41_002768"
     CDS             18518..19870
                     /locus_tag="QIE41_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDS3531642.1"
                     /translation="MNSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAEPDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQPKSNVENAVQTTVRTLIYAPETTASSTPEAVVPPLPDLPEMK
                     VEVRSLSENPPQASVMKQGSTVRGFITGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLGVSPVQPLETGMQMMRVADSRWPESLQQQQASTH
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMRREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDTRAQKQNIDALEKQINERLEGVLIRWLLLKNNVMPEAATGTTAEK
                     "
     gene            19894..20376
                     /locus_tag="QIE41_002769"
     CDS             19894..20376
                     /locus_tag="QIE41_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDS3531643.1"
                     /translation="MANMSYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMVTCEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20420..21334
                     /locus_tag="QIE41_002770"
     CDS             20420..21334
                     /locus_tag="QIE41_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531644.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRRQNTHHVILLTLDVAKKILSSITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDGNGKIIFNFNTLKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACLSFGLPVILVGGAILLTGIVCTVVLNEIDAQYHLSEKLKDAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            21344..21810
                     /locus_tag="QIE41_002771"
                     /pseudo
     CDS             21344..21810
                     /locus_tag="QIE41_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5307 a         5834 c         5892 g         4879 t
ORIGIN      
        1 tccataaaat ggccaaagct gaatcatgaa taaatattgt atttatcctt tctgaattat
       61 cattttgata atttttgaga tattccttaa aaatacccac ttaatcaatc acaaaacaaa
      121 tatataaaca agcaatcatc agattagctt atgtttaatg aaattattat ataattcaat
      181 aagttacata aaaactctat atttaaatta ttgccataat acacctgacg cgaatttcgc
      241 aggttcacac aatctattta acgcttgcag aatacctctc tttacgatcg ataaaaagtc
      301 cttttcttat ttattttgaa gcgtagaatg cggggtgatg aaggagtggt gattttattt
      361 tcaccgccat tagggcggca ttgccgatat ttttttgcgg tacaaatatg ccgtgttcat
      421 ggcctgtacg agataacatg acggatcact tttatgagca aaaaatttga aggcagcgtc
      481 gcgcctcgcg agcgtataaa tatatcctat gtcccgaaga cggacggcca gacggctgaa
      541 gtggagcttc cgcttaacat gctcgttgtc ggcgataccg gaaatacgca ggagacatcc
      601 ccactggatg aacgtcaggc agtttccgtt aacaagcaca atttcggtgc ggtgatggct
      661 gaagcagcca ttggcctcaa ttttaccgtc cccgccacgc tgaagggaag cacaacggac
      721 gatgaactga atgtggcgct gaatatcaag ccactggatg atttttcacc ggacagcgtg
      781 gcccggcagg ttccggaagt taataaattg ctggaattgc gtgaagcact gactgcgctg
      841 aaggggccga tgggcaacct tccggcattc cgtacccagc tccaggcatt gctggaaaat
      901 gaggagtccc gcgagcagtt gctcaaagaa atcggccaag tgagtgggaa ataaccacca
      961 ctatttcatt aaacagggtt atccagacat ggcggcacac aaaaatatct ccgggaaaca
     1021 ccccctctct gacgaagaca ttttgcaggc ggtttaccgg gtatgtttca gcccgtccgg
     1081 tgaaatcaac cgggatggcg tggatgcgct gattacaaca ctggatcgcc gtatcagtga
     1141 tctattggat gagattatcc atctttgtga gtttcagcag agcgcgccgt actggcggcg
     1201 cgtacttcac tgacggcgaa aacccggttt cagaaacaca gctaaaacag aaggattttc
     1261 agtatgtctt tacaggaaga ggaactcgtt tccagtcatg ccgggcagac tgatcaggca
     1321 tcgtcgctgc ttgatcaaat catggctcag acccgtattc agcccggttc agaaggttat
     1381 gacgttgccc gccagggtgt gacagccttt attgccagca tcctgcaatc cactgcatct
     1441 gccgaaccgg tcaataaact ggccgtggac agcatgattg cagatattga tgaacgcatc
     1501 agccgccaga tggatgtcat catccatgcg cctgcctttc agcaggtcga gtcgttctgg
     1561 cgctcactga aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa ggtcaacgtg
     1621 cttcatgtca ccaagcagga acttctggaa gactttgagt ttgcgccaga aattattcag
     1681 tccggatttt ataagcacgt ttattcgtcc ggtttcggac agtttggcgg tgaacccatc
     1741 gctgccgtgc tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa actgctgcaa
     1801 tacgtcagtg ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt ttccccggag
     1861 tttatggggc tgaactcgtg gaccgaactg cctaatatca aagatcttta tgccatcttt
     1921 gaaggtccgg cctacaccaa atggcgcgcc ctgcgtgact cggaagattc gcgctattta
     1981 gggcttacgg caccccgctt cctgcttcgc cagccctatt caccaacaga taaccctgtt
     2041 aagaacttta actattacga agatgtcagc cagaaccacg aagattatct gtggggtaat
     2101 acggcctgga tgctggcatg caatatcgca gacagttttg ccaaataccg ctggtgtcca
     2161 aacattatcg gtccgcaaag cggcggcgca gtgaaagatc tgccggtgca tctgttcgaa
     2221 acgatggggc agattcaggc caaaatccca accgaggtgc tggtcaccga tcgccgcgaa
     2281 tttgaactgg ctgaagaggg ttttatcacc cttaccatgc gtaaagactc tgataatgca
     2341 gcctttttct ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa agacgcagaa
     2401 accaactata agctgggtac gcagcttcct tatctcttca tcatcaatcg gttagcgcac
     2461 tacatcaagg tgttgcagcg tgaacaactg gggtcatgga aagaaaggag cgacttagaa
     2521 agagagctta atacctggat ccgtcagtat gttgccgacc aggaaaaccc gcctgcagac
     2581 gtgcgcagcc gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt agaaggcgaa
     2641 cccggctggt accaggttgc gctaagcgtg aggcctcatt ttaaatttat gggggcaaat
     2701 tttgagcttt ccctggttgg tcggttagac agggagtaac acaccatgat accggatcgg
     2761 gaaagaacca caggttctct ttttgagcgt atggaagcct cttcagccag aaaccggcag
     2821 ggaggcagta ttcattcgct caggcaatcc attcgccaga acctacgaaa cattcttaat
     2881 acccggtccg gtagttgccg gggtgcgcct gagctgggca ttgatgaacc ggaaggggct
     2941 gaaaacttcc gcgaatcaat gagtcgggcc atcgcgcaat gtatagaacg ctatgaaccc
     3001 cggatctcac gcgctgaagt gcaggcagtg gtttcatccg cctccagccc gctggatatg
     3061 acgtttcata ttacagcctg ggtgacattt aacgaaacgc atgaggtgct tgaatttgat
     3121 atggcaccga atggcagcca gcattaccgg gtggactgac aaatggaatt tgaagaacgc
     3181 tatttccggg aagaactcga ttacctgcgc cagcttagca agctgctggc aacggaaaaa
     3241 ccccatctgg cccgcttcct ggccgaaaaa gatgcagatc cggatattga acgcctgctg
     3301 gaaggggtgg cttttcttac cggcaatctc cgccagaaaa ttgaggatga attcccagaa
     3361 ctgacgcacg ggcttattaa gatgctatgg cctaattacc tgcgtccggt tccggcaatg
     3421 acccttattg aatatacgcc ggatatggat aagtcttctg tgccggtgtt aatcccccgt
     3481 aatgagcagt ttacaaccaa cgccggggaa attagagtcg atgaagtgct gccctccgat
     3541 gctaaaaagg aggagccgcc tccctgtacc ttcaccctct gccgggatat ctggctgctg
     3601 cccgttcgcc tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt tatcaacatc
     3661 accttttcgg tcgcaccggg aacggacttc cgcacgctgg atctgaacaa acttcgcttc
     3721 tggctcggca atgacgacaa ctatacccgt gaccagcttt atttatggtt ctgtgaatac
     3781 ttgcagggtg ccgacctgac tgtgggtgaa cagcatattc gcctgcctga gtttatgctg
     3841 aaagctgtcg gttttgagcc acaggatgcc atgctgccct ggccgaaaaa cgtccacagc
     3901 ggctaccgga tccttcagga gtatttctgt tacccggatg cgtttctctt ttttgatctt
     3961 tgtggttgtc cggcgttgcc tgacggattg caggctgaat cctttaccct gcaactgcgt
     4021 ttttcgcgcc cattgcccgt ggacatccgg ttgcgccgcg actccctgcg cctgtattgc
     4081 gcacctgcca ttaatttatt tatccaccat gcagaagcca tcacgctgga caaccggcgg
     4141 gcagactatc cgctggttcc cagccgccat tatccggaac attacgatgt cttttccgtt
     4201 aacggcgtga taagccaggt gcaggatatg ttcaggaaaa aagatctggg gcgtcctgtt
     4261 tcgacgcagg ccgcgcgcca gtggcctgcc tttgaaagtt tcagccatca gatggaatac
     4321 agccggaagc gggaagtggt gtactggcat caccggacca aaacatccct gttccatcgc
     4381 ggctttgatc atacccttgc ttttatacat gctgatggca gttatccgtc agacgaatcg
     4441 ctgctcagta atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga gcttccgtca
     4501 caaattcgtt ccggcgatat caccggcaca accggtaaaa atgcggctgt tgcttcattt
     4561 cgcaacatta cccgcccgac gcaaccgctc tggccggtca ttgatggcag cctgcactgg
     4621 tccctgctct ccgccatgaa cctgaattat ctgtcattac tggatacgga cgcgctgaag
     4681 caggtcatcg ccaactttga tcgccacgca atccatcatc cgcagacggc gcggctgtca
     4741 caacaaaagc tggatgccat tgagcgtctg gagactcgcc ctgtcgaccg cctgtttacg
     4801 ggcattcccg tccgggggct ggcctccacg ctgtatctgc acccggagcc gtttgtctgt
     4861 gaaggggaaa tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct gtacgccagc
     4921 gttaactcat tccacatgct gaccgttgtg aacacagaaa gccaggagac atggaaatgg
     4981 acggaaagaa ccgggcagca tcctcttatc tgagtccggg aaatcccccg gcagataaag
     5041 agcagaatga tccgctggca caggtttttc ataacgcctg ctcttacaat ttttttgcga
     5101 tggcggagct gttgcaccgc ctggcacagg gtgaaaaggg gacgccggaa ttatccctgc
     5161 gcgacgatcc ggcacaggaa accctgcgtt tcagcgccga tgccagcctt gcgtttcccc
     5221 tgagtgatat cagtgcgctg aaacgggata cgtcaggggc attcaggatg accactacct
     5281 ttatggggtt acaggggagc cagtcgcctc tccccggtta ttacctcgat cacctggcct
     5341 ggaaagcggt acatgaacaa agtccggtag gtgattttct ggatatgttc agccaccgcc
     5401 tgacccagtt tgtctggcat atctggcgta agtaccgcta tcacatcagt tttcgtaacg
     5461 gcggtgtgga tgcgttctcg cagcgcatgt actcccttgt tgggctgggc caccgccagc
     5521 ttcgcgataa gctggcgatt aatcacagca aaatgctggc gtattccggg atcctggcaa
     5581 atccgggacg atccccggaa ataatctgcg gtctggtgtc ccactgtttt gatttgtcag
     5641 acgtgacgct gcaaaactgg caacgtcgca aggtggatat tgaaccggat cagcaaaata
     5701 gcctggggag ttattcactt aaaaagggcg aaaaactggc aggaagatcg gtactgggca
     5761 actttgttct gggtacacgg gtgcccgatc tcagcggaaa attccagctc agtattacca
     5821 gcctgacccg aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc ctgccactga
     5881 cgatgtttgt gtccttcatc ctgcgcgatc agcttgcctg ggacttacat ctggggctgg
     5941 cgccggagca ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg ctgggctgga
     6001 ccagcttcct cggcacaccg gaagaacgac cttcagtcac catcagggta cggtcgtaaa
     6061 ttatgataat cacggagcag aatatgccgg aagagaaact acagacgctt tcattgcagg
     6121 tcattaacgg cagtgagctg gaaagcgggc gggcggcgcg ctgtctgttc acacagcagg
     6181 gaaatgtcgg ccatggcccc gaatgccact ggtcggtaca ggatcgtcag cagagcattc
     6241 cggctcaggc ttttaccgtt atcctgcacg atggcacatt ttgtctgcgc ccgcagacgg
     6301 cacaactgtg gctgaaccag gcaaaagtca cagcaacatc agacctgata cagctgcgcc
     6361 agggcgatga gatccagata gggcgtctga tggtgagggt tcatctgaac cggggagata
     6421 tcccccatta cgatgaggaa atggccactc ccgaaaccat cgtcaccaat cgcgatatgc
     6481 tcacggatac cctgctgtca acggagggtg cgccacacta tccgggaatg actcaccggc
     6541 atcagcttgc agacaccgtg gtaaacggtt tttctaccga tccactccag gcacttcagt
     6601 ccgaaagcct gattaccacg ggcgatccgc tttcaggcat tgcggctgtc cggccatcgg
     6661 caccgctgtc cgatccggca agtaatggtg ggatcaatac tccgtttatg gatctaccgc
     6721 ccatttatgc cagccccggc gatcgtaatg atgacttccc tgtggcagaa atggcgcaac
     6781 gccaccttgc ggtcaccccc ttactgcgcg gtctcggcgg ctcgcttacc gtgagcaatt
     6841 ctgacgatgc ggatgatttt ctggaagagg ccggacgcac gttacaggcc gcaataaaag
     6901 gtctgctcga tttgcagcaa cagcgtaaca gcctctcaga caaacatttg cgcccgctgg
     6961 aagataaccc gctgcgcctg aacatggatt acgccaccgc gctcgacgtg atgtttgccg
     7021 aaggtaaaag tccggtacat ctggcggctc ccgccgctgt cagtgaaagc ctgcgtaatg
     7081 tccgccacca cgaagaagct aaccgggcag cgattgtgga gtcacttcgt gtcctgcttg
     7141 acgctttctc accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc agccatgaac
     7201 tgcgccagcc gctggatgat gccggagcat ggcaaatgta cagccattat tacgaagaac
     7261 tggcctccga tcgccagcag gggtttgaga tgctgtttaa cgaggtctac gcccagatct
     7321 atgaccgggt gcttcgtgaa aaacagcggg agccggaagc atgaacagga aggcgtttct
     7381 ggcctgcgtg ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa aaatcagcga
     7441 ggtgatcaaa aacccggata tccaggtcgg gagcctgaag gagcaacctt cggaaataac
     7501 cgtcaccctg ctgaccgaac cggacaccaa taccaatgcg gagggtgaat cagccgccgt
     7561 ggatgtgcaa ctggtgtacc tgactgacga ctcaaaattg ctggcagccg actatgacca
     7621 gatagccagt acgccgctac cggatgtgtt gggtaaaaac tatatcgacc accaggattt
     7681 caacctgttg ccggacacca tcaaaaccct gccgccggta aagctggatg agaaaacgca
     7741 gtttattggt gtcgtggcct atttctccga tgaccaggcc actgaatgga aacaaatcga
     7801 gacggtggaa ggaaccggcc accattaccg cctgctggtg catgtacgtc aaagcagcat
     7861 cgaaatgaaa aaagaggatg aataattatg gcaaccaccc gcaacaaggt gatgtggcag
     7921 gaagggatgc tgatgcgccc acaccatttc cagcaacagc agcgttacaa cgactacctg
     7981 gataaccagc gtttccgggc catgaatgat ttatcctggg gatttaccga actcaccctc
     8041 aacaatgaac tgctggcgca gggtaagatc atgattgaca gcgcgtcagg cacactgccc
     8101 gacggcaccg tcttttctat ccccgaccag gacgcactgc ccgatccgct gcacccgcaa
     8161 cattttccgg acgaaagaag ccgcaatatt tacctcgctc tgccggtcgc cagtgatgtg
     8221 agaaatgaaa tcagcgacgg gcggcgaatc gggcgttacc ggctgaatta tgccgatgtc
     8281 cgggatctgc attcagaaga aggtgacgcg cgaacactga cgctgggtca actgacgccg
     8341 cgcattatga gcggtgcaga agatatgagc gcctatatta cgctcccgct ttgccgtatc
     8401 agcgatcgcc atgccgacgg ctccctgacg ctggatgacg attttatccc ctcctgccag
     8461 aatattcagg tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg ggccattggc
     8521 ggacgggcaa gcgatctggc aaaccgcatt ggctctccgg cgcagagcgg catcgcggat
     8581 gtggcggaat ttatgatgtt gcagttactt aaccgtaacc agacccggtt tacccatcgc
     8641 gctcgtcgat cccagctcca cccggaagat ttctaccttg atcttgccgg gttgctgggt
     8701 gaactgatga cctttaccga gccgtcgcgc ctgccctgcc cgcttgatgt gtatgatcat
     8761 catgacctga ccaaaacatt taaaacgctg ttaccggaag tcaaacgggc gctgcatacc
     8821 gtactgtcgc caagagccgt taatctgccg ctgcatctgc gtgacggtat ctggcaggcc
     8881 gatatccatg acacagaact gctgcaatct gccacctttg tgctggccgt ggcggcaaac
     8941 atgccggtcg atcagatcca gcgccagttt atccagcagt cgaaaatttc ctcgccggaa
     9001 aaaatccgca atatggtcag tgtgcagata cccggcattc cattgcgtgc cctgatggtg
     9061 gctccccgcc agcttcctta ccattccggg ttcagctatt tcgaactcga caagagcggc
     9121 caggcctgga cagaaatggc tgccgccggg gccgttgcac tgcatgtatc cggcagtttc
     9181 ccggatctga acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact
     9241 gtctctgcca ccatttaccg gacaccacga aaaaagccag cgcgactatc atctcgcgct
     9301 tcgtggcaac agcctgaatc cgatgatcga cgcagccacg ccgctcctcg gtatggtgat
     9361 gcgcctttca accatgaaca gtcagacgat gccggagcat ctttttgctc aggtggtgac
     9421 agatgttcag gccgtggagc aactgcttca ggagcagggc tacgaaccgg gcgtgattat
     9481 ctcgtttcgc tacatcctct gcacgtttat tgatgaagcg gcgctcggta acggctggtc
     9541 gaacaagaac gagtggatca agcagtcatt gctggtccat ttccacaacg aagcctgggg
     9601 tggagagaaa gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga
     9661 tctgctggaa tttctctgga tctgtttttc tctgggattt cgcgggcgct acaaagtggc
     9721 agcacaggat cagggggaat ttgagcagat ctaccgccgt ctgtaccacg ttctgcataa
     9781 actgcgcggc gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc agggcgggcg
     9841 ttatcagctc atcagtcgcc tgactgttaa acatattttt tgtggtggcg ttgtcgtgct
     9901 ggcgttgttt tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca
     9961 ccagctaaac aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg
    10021 gtaaaacggc tgaacctgtt ctcccgccag gcgctggaga tggccgcctc tgaatgtatg
    10081 agtcagcagg cagcggaaat taccgtcagc catgtgctta ttcagatgct cgccatgcca
    10141 cgcagcgacc tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag
    10201 gcgctgacgg tggagaacta cacaactgcc cgttctgcgg acagctaccc ggcattttcc
    10261 ccgatgctgg ttgagtggct gaaagagggc tggctgctgg cgtcggctga gatgcagcac
    10321 agcgaattgc gtggcggcgt gttgctgctg gccctgctgc attcgccgct gcgttatata
    10381 ccgcctgctg ccgcccggtt gttgaccggc attaaccgtg accgtctgca acaggacttt
    10441 gtacagtgga cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaagtcgca
    10501 ggcacactga cggacgctgc tgacaccctg cttgcccgct acgccaaaaa catgaccgca
    10561 gacgcccgta acggcaggct tgacccggta ctgtgccgcg accacgaaat cgacctgatg
    10621 atcgacattc tctgccgccg ccgcaagaac aacccggtgg tggtgggcga agcaggcgtg
    10681 ggtaaaagtg ccttaatcga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac
    10741 aaactgaaaa acaccgatat catgaccctt gacctaggcg cattgcaggc cggggcgtcg
    10801 gtgaagggcg aattcgaaaa acgcttcaag gggctgatgg cggaggtcat ttcctctccg
    10861 gtgccggtca ttctgtttat tgacgaagca cataccctga ttggcgcggg caaccagcag
    10921 ggcgggctgg atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga actgaaaacc
    10981 atcgccgcca ccacctggag cgaatacaaa aaatacttcg agaaagatgc cgccctgtcg
    11041 cgccgcttcc agctggtgaa ggtcagcgaa cccaacgccg ccgaagccac cattattctg
    11101 cgtggtctgt cggcggtcta tgaacagtct cacggcgtgc tgattgatga tgacgccttg
    11161 caggccgcag caacgctgag cgaacgttat ctctccgggc gtcagttgcc ggacaaagcc
    11221 attgatgtgc tggataccgc ctgcgcccgc gtcgccatta acctttcctc gccgccgaag
    11281 caaatctcgg cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag
    11341 cgcgagcttc gcatcgggct gcgtaccaac acctcacgga tgaccgaggt gctggtgcag
    11401 tacgatgaaa cgctgacggc gctggatgaa ctggaagcgg cctggcatca gcagcaggcg
    11461 ctggtccggg agattatcgc gctgcgccag cagttgctgg gcgtggcaga agacgatgcg
    11521 gcgtcgttgc cggacgcaga tgccgcggag gatacgccgc cagagtcaga gtcagagtca
    11581 gagtcagagt cagagtcaga gtcagagtca gagtcagagt cagaacagga taataccggt
    11641 gccgtaccgg ctgatgaggc cgacagagaa cagccggaag agaccgctga aacagtttcc
    11701 ccggtacagc ggctggccca gctcactgcc gaactggacg ccctgcataa cgaccggttg
    11761 ctggtctccc cgcacgtcga taaaaaacag attgcggcgg tgattgccga atggaccggc
    11821 gtgccgctca accgcctgtc gcagaatgaa atgtcggtca tcaccgacct gccgaaattg
    11881 ctgggcgaca ccatcaaagg ccaagacctg gcgattgcca gcctgcataa gcatctactg
    11941 accgcacgcg ccgacctgcg tcttccggga cgcccgctcg gtgcgttcct gctggctggc
    12001 cccagcggtg tgggtaaaac cgaaaccgtc ctgcaactgg cagagctgct ctacggcggt
    12061 cgccagtacc tgaccaccat caatatgtcc gaattccagg agaaacacac cgtctcgcgg
    12121 ctgattggtt cgcctccggg ctacgttggc tacggtgaag gcggcgtact gaccgaagcg
    12181 attcgccaga aaccctactc ggtagtactg cttgatgaag tggaaaaagc gcacccggat
    12241 gtgctcaacc tgttctacca gtcgttcgat aagggcgaaa tggcagacgg tgaaggccgc
    12301 ctgattgact gcaaaaatat cgtcttcttc ctgacgtcca acctcggcta ccaggtaata
    12361 gtcgagcacg ccgatgaccc ggaaaccatg caggaagaac tgtatccggt gctggccgac
    12421 ttcttcaaac ctgccctgct ggcgcgtatg gaagtggtgc cgtatctgcc gctgtcgaaa
    12481 gagacgctcg ccaccattat cgccggaaaa ctggcccgtc tggataaagt gctgcgcagt
    12541 cgctttggtg cagaagtggt cattgaaccg gaagtgacgg acgaaatcat gagccgcgtc
    12601 acccgcgcgg aaaacggcgc gaggatgctg gaatcggtca tcgatggcga catgctaccg
    12661 ccgctctcgc tgctgctgtt gcagaaaatg gcggctaaca cggcgattgc ccggattcgg
    12721 ttgtcggcgg tggacgacgc atttacggca gacatggaag atgctcagaa cgacgagtcc
    12781 gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt tttccggcag acaggatggt
    12841 ttatcgcggg cctgatgacg ggcttacccg ccactgccgc gcccgcagaa acatcgtcaa
    12901 tggccggtgt ggcggtcgcc gtcgccacca cgaccccgcc ggatgcgaca gccacgtcac
    12961 aggccatgca gtcctgtcgg cgggagtccg cggcgctgga gcggcttgac tgctatgacc
    13021 gcctgctggc accgctgtcc ccgtcagggt ttgacggggc gctggtcaaa gccggttttg
    13081 tgggcgaggc ctggacccgc gccacagagc tggaaaaacg ccgcgagggc aacaccacag
    13141 aactgctggt gacgcaggtg ccgggggagc gcccgacggt ggtgataacc accccggcta
    13201 tcggccatgt tccgccgcgc ccggtgctga tgttcagttg cgtggacaat atcacccgga
    13261 tgcaggtggc gctgatgcac cctctggatg tccatgatat cgccgtcact ctgaatgccg
    13321 acagccgggc attgcgtagc cactggtttg tgcgcgagaa cggcaccctg ctggagtcca
    13381 gccgggggct gtccgggatt gatgaaatta agcaactgtt cggcgcaaaa acgctgacag
    13441 tggataccgg cgcagataac gccgcaggaa agctgacctt taacattgac gggctggcaa
    13501 gggcgattgc ccccctgcgc gttgcctgcc actgggcggg agaataaaaa tatggcaatg
    13561 gacttacgcg atccgaatgt ctggataacg cacctgctgg aaaacctgcc ggaagaaaaa
    13621 ctggcatcgg cgctgaaaga tgacaacccg gactgggagt atatcgacgg cgaaatcgtc
    13681 aaactgggat ctctggccca cagtcaactg gatatcccgg aactccagcg ccgggggctg
    13741 gtcattctgg cttcggagag taaggacttt cgtctgctgg cgcatctgct gagaaccctg
    13801 caacacgccg gtgatcctca tctcgcattg cgcctgctgg cgctgtatgt ggagcattac
    13861 tggtcggtgg ctgcgccgca gaatatggca cataaaaaac gctttgccag ccaggtcatt
    13921 aaacgcttcg aaacaggtat tgaaggcttt tcacagaacg ccgccacagc gcagcgcgac
    13981 gcgctgtcag gggaactggc gaaactggcg cagtgctggc agtcacataa cgccccggaa
    14041 ctggcactgg ctaccgatga tctttttgcc ctgtaccagc gtgcgtttaa tcgtgcggca
    14101 ccggctcctg cccccactcc agcggcctcc gatagttcac gacagaccac cgccacgtct
    14161 gaaagcggcg tgacgcaacc cagtgctccg gctccccaaa tcgccatcga cagtcacgac
    14221 gacaaagcct ggcgcgacac gctgttaaaa gtggcggcta ttttatgtga acgccagccg
    14281 gactcgccgc agggctatcg cctgcgccgc catgccctgt ggcaatccat caccagtaca
    14341 ccccaggcgg aaagcgatgg acgtacccca ttggctgcgg tctctgccga tatggtggcg
    14401 gattaccagt cccggcttgc cagcgcggat atggcgctgt ggcagcaggt tgagaaaagc
    14461 gtattgctgg ctccttactg gctggacggt cactgtcttt ctgcacagac ggcactgcgt
    14521 ctgggttaca aacaggtggc agacaccatc cgcgatgagg tcatccgctt ccttgagcgt
    14581 ctgccccagc ttaccgggct gctgtttaat gaccgcacac cgtttctcag tgagcagacg
    14641 aaacaatggc tggcagcttc gcccgacggc aaagttgcac cggttgcgca aatcggtgag
    14701 gaatcgcagg cagccagagc ctgttttgct gggcagggtc tggaggcggc gctgcgatat
    14761 ctggacatgc tacccgaagg cgatccccgc gatcagtttc accgccagta ccttgccgca
    14821 cagttgacgg aggaggcggg gctgatacag cttgcgcagc aacagtaccg gatgttgttg
    14881 atgataggga gtcagatgat ggtgtctgac tgggagccat cattacttac gcagcttgaa
    14941 caaaaattca cggcagaaca ataaaagaga acaggagttt ttgtgttcag attaccaaca
    15001 ccccgactac tcagcggact caaatcagcc ctacgcccgg cgatgccccg gtttaaagtc
    15061 tccgctttct ggctgctgat actggcgtgg atttttctgc tggtatggat ctggtggaaa
    15121 ggcccgacgt ggacgctgta tgaagaacag tggctcaaac cactggcgaa ccgctggctg
    15181 gcaacggcgg catgggggat tatcgcccta gtgtggctta ccgtccgggt gatgaagcgc
    15241 ctgcaacagc ttgaaaaaat gcaaaagcag cagcgcgagg aggccattga tccgctcagt
    15301 gtggagctga acgcccagca gcgttacctt gaccgctggc tgctgcgcct gcaacgccat
    15361 ctcgacaacc gccgtttcct gtggcagttg ccgtggtata tggtcatcgg cccggcgggc
    15421 agtggcaaaa cgacactgct gcgcgagggt tttccgtccg acattattta tgccccggag
    15481 ggcgcacgcg gcgcagaaca acgcctgtat ctcacgcccc atgtcggtaa acaggcggtg
    15541 atctttgata tcgacggcac actctgcgct cccgctgatg cggatatcct gcatcgccgt
    15601 ctgtgggaac atgccctcgg ctggctgaaa gaaaagcgcg cgcgccagtc gctgaacggg
    15661 attattctga cactcgattt acccgatctg cttaccgcag acaaacgccg ccgcgaacat
    15721 ctgttacaga cgctgcgcag ccgcttgcag gatatacgcc agcatcttca ctgccagtta
    15781 ccggtttacg tggtactcac ccggcttgat ttattgcagg gttttgccgc cctgttccag
    15841 tcactgaaca gacaggatcg cgatgcaatt ctaggggtca cgttcacccg ccgcgcccat
    15901 gaaaatgatg actggcgaac agagttgaat gctttctggc agacatgggt ggatcgaatg
    15961 aacctagcgt tgccggatct gatggtcgct cagactcaca cccgcgcgtc tttatttagt
    16021 ttttcccgcc agatgcaggg aagccgtgaa ccactggtgt cactgcttga gggtctgctt
    16081 gacggcgaaa atatgaacgt gatgctgcgt ggcgtctatc tcacctcttc gcttcagcgc
    16141 ggacagatgg atgatatttt cacccagtct gccgcccgcc aataccggct gggcaataac
    16201 ccactggcgt cctggcctct ggtggacacc gcaccctatt tcacccgtag cctgttcccg
    16261 caggcgttac tcgcagagcc taatctggca acggagagcc gcgcctggct gatgcgttcc
    16321 cgtcgccgcc tgacggtttt ctctgccact ggcggcgtgg cggcactgct gctcatcacc
    16381 ggctggcatc actattacaa tggtaactat cagtccggca tcaccgtgct taagcaggcc
    16441 aaagccttta tggatgtgcc gcctccgcag ggggaagatg actatggcaa cctgcaactg
    16501 ccgctgctga acccggtacg cgatgccact ctggcctacg gtgactgggg cgaccgcagc
    16561 cgccttgcgg agatgggatt atatcagggg cgacgcatcg ggccttatgt ggaacggacc
    16621 tacctgcaac tgctggagca acgctatctt ccgtcgctgt ttaacgggct ggtcaaagag
    16681 atgaacgccg cgccaccgga gagtgaggaa aaactcgccg tgctgcgcgt gatgcgaatg
    16741 ctggaggata agagcggacg taacaatgag gtggtgaagc agtatatggc aaaacgctgg
    16801 agcgaaaagt tccacggcca gcgcgatatc caggcgcaac tgatgtccca tcttgactac
    16861 gcgctggctc acacagactg gcatgcagag cgtcaggcgg gcgacggtga cgctatcagc
    16921 cgctggacgc cgtatgacaa gcccgttgtg tcagcacaga aagaactaag caaactacct
    16981 gtctaccagc gagtttacca gagcctgaaa acgcgggcgc tgggcgttct tcctgccgac
    17041 ctcaatctgc gtgaccaggt agggccaacc tttgaccagg tgtttacatc tgccgatgac
    17101 aataaactgg ttgttccaca gtttcttacc cgttacggcc tgcaaagcta ttttgtaaaa
    17161 cagcgcgatg aactggttga actgacggcg atggattcct gggtactcaa cctcacccgc
    17221 agcgtgaaat acagtgacgc cgaccgggcg gaaatccagc gccagttgac cgagcagtat
    17281 atcagcgact acaccgccac ctggcgggct gggatggaca atctgaatat ccgcaatttt
    17341 gagtccatcg gacaactgac cggggcgctg gagcaggtta tcagcggtgg ccagcctttg
    17401 cagcgggcgc tgaccgtgct gcgtgacaac acacaaccag gcgccttttc tgaaaagcta
    17461 tctgctaaag aacgggatga ggccctggct gaaccagact accagttact cacccgcctt
    17521 gggcatgagt tcgccccgga aaacagtacc ctgacagtcc agaaagataa agaaagcacg
    17581 atgcaggccg tgtacctgca gctcaccgag ttacaccgct acctgctggc gatccagaac
    17641 gcgcctgtac ctgggaaatc ggcactgaaa gccgtgcagt tacggcttga tcagaacagc
    17701 agcgatccca tattcgccac ccgccagatg tcaaaaacac tgcctgctcc gctcaaccgc
    17761 tgggcaggca ggctggctga ccaggcctgg catgtggtga tggtggaagc cgttcactat
    17821 atggaagtgg actggcgcga cagcgtggtg aaaccgttta acgagcaact ggcaaataac
    17881 tatccgttta atccacattc tgcacaggat gcctcactgg atgccttcga acgattcttt
    17941 aaaccagatg gcatactgga taccttctac cagcagaacc taaagctgtt tatcgacaat
    18001 gacctgagtc tggaggatgg cgataacaac gtcattattc gcgaagatat tattgcgcaa
    18061 ctggaaaccg cgcagaaaat ccgtgacata ttcttcagca aacagaacgg tctgggaaca
    18121 tcctttgccg tggaaacggt atcgctttcc ggcaacaaac gccgcagcgt actgaacctt
    18181 gacggtcagt tagtcgatta cagccagggc cgtaactata ccgcccatct ggtctggcct
    18241 aacaatatgc gcgaaggcaa cgaaagcaaa ctgacgctgg ttggtgcccg cggcggcgca
    18301 ccgcgcagta tcagcttcag cgggccgtgg gctcagttcc gcctgttcgg ggccggacag
    18361 ctgaccggag tacaggatgg caactttacc gtgcgcttta gtgtggacgg tggcgcaatg
    18421 acctaccgcg tgcataccga cacggaagat aacccgttca gcggtgggtt attcagccag
    18481 tttggcctgt cagacacact gtactgagga ggcagggatg aatagtaacg gactgacaca
    18541 aactatcgtt accggcagtg acccgcgcgg gctgccggaa ttcagcgcca tccgcgagga
    18601 aataaacaaa gccagccatc cgtcacagcc tgagctgaac tggaaactgg tggagtcgct
    18661 ggcgctggcg atttttaaag ccaacggtgt ggatttacac accgccacct actatacgct
    18721 tgcccggaca cggacacagg gactggcggg attctgcgaa ggtgcggaac tgctggcggc
    18781 aatggtaagc cacgactggg ataaattctg gccgcagggc ggtccggcgc gtactgaaat
    18841 gctggactgg tttaactccc gaaccggcaa tattctgcgt cagcaaatct cctttgcgga
    18901 acccgatctg ccgctgatct accgcacaga gcgggcattg cagcttatct gcgacaagct
    18961 tcagcaggtg gagctgaagc gcgttccgcg cgtggagaat ctgctctatt ttatgcagaa
    19021 cacgcgtaag cggcttgaac cccagccgaa gagtaacgtt gaaaacgccg tacagaccac
    19081 ggttagaaca ctgatttatg ccccggaaac aacggcttcc tccacgccag aagcggtagt
    19141 gcctcccctg cccgacctgc ctgagatgaa agtagaagtg cgcagtctgt cagagaatcc
    19201 cccacaggcc agtgtgatga agcaaggcag tacggtaaga gggtttatca cggggatcgc
    19261 ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag gtctatccgg tgcaacagca
    19321 actgttacag gttaacgaca ccgcgcaggg cgcagcaacg gtgtggatgg cctcacctga
    19381 actagaaaac tatgagcgca ggctgcaaca gcttcttggc gtctccccgg tacaaccgct
    19441 ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc tggccggaaa gcctgcagca
    19501 gcagcaggcc tcgacacatt ggaatgaagc actcaaaacc cgcgcacaga gtagcccgca
    19561 gttgcgtggc tggttgcaga cccgtcagga cttacatgct tttgcagatc tcgtgatgcg
    19621 gcgcgagaaa gagggactaa ccctttccta tatcaaaaat gtcatctggc aggcggagcg
    19681 gggactgggg caggaaacac ccgttgagtc tctgttgacg cagtaccagg atacccgtgc
    19741 gcagaagcag aatatagatg cgctggaaaa acaaattaat gagcgactcg aaggcgtgtt
    19801 aatccgctgg ctgctgctga agaataacgt catgccagag gcggcaaccg ggaccacggc
    19861 cgaaaaataa ttatttaatt aaaggagatt atcatggcaa atatgagtta tttatctttg
    19921 agtggagaga cacaagggct gatttcagca ggttgctcaa cgcttgattc tgtcggaaac
    19981 aaagcacagc cagaacataa agatcagatc atggtttatg cgttaatgca ttccatctca
    20041 agaagtcaaa atgtcaatca tcatgaatta attatcacca aaccggttga taaatcctca
    20101 ccattgctgg caaaagcact tagtgacaat gaaaaaatgg taacatgtga atttatactc
    20161 taccgaacca gtaaagcggg tatttaccag ccttattata aaataaacct gtcaaaagcc
    20221 cgaatatcaa gtattgattt tgtcacaccg catgctgtcc tggaaaaaga actggagcca
    20281 caggagagaa ttgcctttat ttatgaagat atttcctggg agcatacgtt agccggtacg
    20341 aatgcaatga gtaaatggca ggatcgtgta cagtaattta ctgtaataac ataataaatt
    20401 actattgcgg agagatataa tggctggttt aaggaataat aacaacacac agaatgcaca
    20461 gtgggctgat tatgtgggtg acattcttcg gggggctcaa ccgataaacc agctagtacc
    20521 tcagcaccct tatcttaacg acgtcccact cattgatgaa ttaaggcgtc agaacactca
    20581 tcatgttatt ctcttgacac ttgatgttgc aaaaaaaata ctcagctcaa taacatcatt
    20641 tgattacatt cattttatca ctacacatcc ttcaggtata aaagatacac tcgcttggtt
    20701 agttaacgcg ggtaaattaa tgactgagtt tgatggaaat gggaaaatta tatttaactt
    20761 taatacctta aagtatacaa aagcaagtta ctttgaaata cttggagaga aatatataaa
    20821 aattacaaca tccagcccct ggcttttaga aaagctcggt aaatatattt tctcttcacg
    20881 agcgcctcaa gttctggaac tcgctattgg ctggcgagga gctttatctg aatcaataaa
    20941 aggagttaag ttctgtatct ggttttcagt agcctggaga accatagaat ttattatgag
    21001 ttcagagcga gatctggtta attttcttgg tgatttttca atggatgtgg ccaaagcagt
    21061 gattgcaggc ggtgttgcaa ctgctattgg aagcctggct tcttttgcct gtcttagttt
    21121 tggccttcca gtaattcttg taggaggagc aattttactt acagggatag tgtgtacagt
    21181 tgttttaaat gaaatcgatg ctcaatacca tttatcagaa aaattaaaag atgcaattag
    21241 agatggacta aaacggcaac aggaacttga taaatggaaa agggaaaaca tgactccatt
    21301 tatgtatgtt cttaacactc cccccgtgat atgaggatta actatgcgtt tacatgttaa
    21361 attgaaagag tttttttcga tgttttttat agccatatta tttttccctg cattcaatgc
    21421 ttctttattt tttaccggaa ttaaacctct atattcaata ataaaaggct ccacggaaat
    21481 attttatgat tggagaatgt tgattctatg cttcggtttc atatcgtttt ccttgttaaa
    21541 tattcatgta attttactaa caataataaa atcattctta ataaaaaaac aaaggtaatc
    21601 aatttcgcca cagacataac aatacagcta actcttatat ttttactaat agctatcgta
    21661 atagcacctt taatagctcc ttttgtcaca ggatatgtca atgctaatta tcacccctgc
    21721 ggtaataaca cggggatttt cccaggagca atctatatta aaaatggcat aaaatgtaat
    21781 aatgaacata tttctcgcaa ggaagattaa gcagttaaat aattaaatat tctaaaacaa
    21841 acagttttta acgccggatt ccatgtcctt tcttgcactg tctttgtcag gaacaatttc
    21901 ttatgcaaag aa
//
LOCUS       DAOTRK010000077        21684 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.077, whole genome shotgun sequence.
ACCESSION   DAOTRK010000077 DAOTRK010000000
VERSION     DAOTRK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.077"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(11..985)
                     /gene="lepB"
                     /locus_tag="QIE41_002772"
     CDS             complement(11..985)
                     /gene="lepB"
                     /locus_tag="QIE41_002772"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDS3531645.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(1001..2800)
                     /gene="lepA"
                     /locus_tag="QIE41_002773"
     CDS             complement(1001..2800)
                     /gene="lepA"
                     /locus_tag="QIE41_002773"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDS3531646.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(2998..3477)
                     /gene="rseC"
                     /locus_tag="QIE41_002774"
     CDS             complement(2998..3477)
                     /gene="rseC"
                     /locus_tag="QIE41_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDS3531647.1"
                     /translation="MIKEWATVISWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLMGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(3474..4430)
                     /gene="rseB"
                     /locus_tag="QIE41_002775"
     CDS             complement(3474..4430)
                     /gene="rseB"
                     /locus_tag="QIE41_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDS3531648.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(4430..5080)
                     /gene="rseA"
                     /locus_tag="QIE41_002776"
     CDS             complement(4430..5080)
                     /gene="rseA"
                     /locus_tag="QIE41_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDS3531649.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(5113..5688)
                     /gene="rpoE"
                     /locus_tag="QIE41_002777"
     CDS             complement(5113..5688)
                     /gene="rpoE"
                     /locus_tag="QIE41_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDS3531650.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(5685..5840)
                     /gene="rseD"
                     /locus_tag="QIE41_002778"
     CDS             complement(5685..5840)
                     /gene="rseD"
                     /locus_tag="QIE41_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDS3531651.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            6096..7718
                     /gene="nadB"
                     /locus_tag="QIE41_002779"
     CDS             6096..7718
                     /gene="nadB"
                     /locus_tag="QIE41_002779"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDS3531652.1"
                     /translation="MNTLPDHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGGGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNICVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMYLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(7703..8440)
                     /gene="trmN"
                     /locus_tag="QIE41_002780"
     CDS             complement(7703..8440)
                     /gene="trmN"
                     /locus_tag="QIE41_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDS3531653.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAKRINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            8572..9906
                     /gene="srmB"
                     /locus_tag="QIE41_002781"
     CDS             8572..9906
                     /gene="srmB"
                     /locus_tag="QIE41_002781"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDS3531654.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(9939..10820)
                     /locus_tag="QIE41_002782"
     CDS             complement(9939..10820)
                     /locus_tag="QIE41_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3531655.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIKDVDFTEPGRHNACSFIINEPQCVFRQLFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFLPG"
     gene            10923..11510
                     /gene="eamB"
                     /locus_tag="QIE41_002783"
     CDS             10923..11510
                     /gene="eamB"
                     /locus_tag="QIE41_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDS3531656.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCVGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRPLNIVLALLLVYCAVRIFY"
     gene            complement(11572..11955)
                     /gene="grcA"
                     /locus_tag="QIE41_002784"
     CDS             complement(11572..11955)
                     /gene="grcA"
                     /locus_tag="QIE41_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDS3531657.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            12260..12949
                     /gene="ung"
                     /locus_tag="QIE41_002785"
     CDS             12260..12949
                     /gene="ung"
                     /locus_tag="QIE41_002785"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDS3531658.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            complement(12997..14034)
                     /locus_tag="QIE41_002786"
     CDS             complement(12997..14034)
                     /locus_tag="QIE41_002786"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDS3531659.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSHPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDEALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            14241..14660
                     /gene="trxC"
                     /locus_tag="QIE41_002787"
     CDS             14241..14660
                     /gene="trxC"
                     /locus_tag="QIE41_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDS3531660.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            14729..15427
                     /gene="tapT"
                     /locus_tag="QIE41_002788"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             14729..15427
                     /gene="tapT"
                     /locus_tag="QIE41_002788"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDS3531661.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            15459..18119
                     /gene="pat"
                     /locus_tag="QIE41_002789"
     CDS             15459..18119
                     /gene="pat"
                     /locus_tag="QIE41_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDS3531662.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            18233..19588
                     /gene="pssA"
                     /locus_tag="QIE41_002790"
     CDS             18233..19588
                     /gene="pssA"
                     /locus_tag="QIE41_002790"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDS3531663.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            19634..19957
                     /locus_tag="QIE41_002791"
     CDS             19634..19957
                     /locus_tag="QIE41_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDS3531664.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSATFGLMFSVGLGASKELWDSRPEGSGWSWKDFTWDVAGATTGYTVW
                     QLTRH"
     gene            complement(19954..21252)
                     /gene="kgtP"
                     /locus_tag="QIE41_002792"
     CDS             complement(19954..21252)
                     /gene="kgtP"
                     /locus_tag="QIE41_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDS3531665.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(21561..>21684)
                     /locus_tag="QIE41_002793"
     CDS             complement(21561..>21684)
                     /locus_tag="QIE41_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDS3531666.1"
                     /translation="VEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG"
BASE COUNT         5283 a         5696 c         5416 g         5289 t
ORIGIN      
        1 agatgggcta ttaatggatg ccgccaatgc gacttaagcg cacacctgtc ggccattcgc
       61 cttcttgctt atcgaagctc atccagatag ccgttgcccg accgaccaga ttcgcttccg
      121 gcacaaagcc ccagtaacgg ctgtccgcgc tgttgtcgcg gttgtcaccc atcatgaagt
      181 attgtcccgg cggaacaatc caggttgcca gttgttgccc tggctgctgg taatacatcc
      241 ccacctggtc ctgcgcaatc ggcactgtca gaatgcggtg cgtcacatca cccagtgtct
      301 ctttacgctc ggaaagacga attccatttt ctttggtttc gtttttcggc acttcaaaga
      361 atccgctggt cgcttcccca ccattacggc gtgagaaggt ctgaacgaaa tcgctcggtt
      421 ccacgtttga gtaggtgacc ggcagcgcgt tttcacatgc ctggccggaa ctacatcccg
      481 gttgaatcgt cagctctttt gagaccggat cataagtgac tttatcgccc ggtaaaccca
      541 ccgcgcgctt gatgtaatca agctttggat cttccggata tttaaagacc acgatatcgc
      601 cgcgtttcgg atgaccggtt tcgatcagcg ttttctggta gataggatct ttaatgccat
      661 aagcaaactt ctctaccaga ataaaatcac cgattaacag agtcggcatc atcgaacctg
      721 acgggatctg gaacggttca taaataaacg aacgcacaat caatacgata gccagtaccg
      781 gaaaaacaga agcaccggtt tccagccagc caggcttcgg cgcaaccttt ttcaacgttg
      841 ctttatccag tgagtcaccg gcagccgcct gcgccgctgc ctgacgttcc cgccgtttag
      901 gtgcgaaaaa gaatttatcc acgcaccata aaatgcccgt caccagtgtg gcaatcacca
      961 gaatcagggc aaacatattc gccatgccaa ctcctaaggg ttatttgttg tctttgccga
     1021 cgtgcagaat ggcgaggaac gcttcctgcg gcagctcgac gttaccgatc tgcttcatgc
     1081 gtttcttacc ttctttctgc ttctgcagca gctttttctt acggctgata tcgccgccat
     1141 aacatttagc cagtacgttt ttacgcagct gtttcacggt ggatcgcgca ataatgtgcg
     1201 taccaatcgc tgcctgaatg gcgatatcaa actgctggcg tgggatcaga tctttcatct
     1261 tctccaccaa ctcgcgaccg cggttttgcg aattatcacg gtgggtgatc aacgccagcg
     1321 catcaacacg ttcaccgttg attaatacgt ctacacgtac catgtcggac gcctggaagc
     1381 gtttgaagtt gtaatccaga gacgcataac cacgcgaggt agatttcagg cgatcgaaga
     1441 agtcgagcac cacttccgcc atcgggatct cgtacgtcag cgccacctga ttaccgtggt
     1501 aaaccatatt ggtctgcacg ccgcgttttt ctacgcacag cgtaataacg ttgccgagat
     1561 atgcctgcgg cagcagcatg tgacattctg caatcggctc gcgcagttcg tagatgttat
     1621 ttaccgctgg cagcttggat gggctgtcga cgtagataac ttctcttgac gtggtttcaa
     1681 cttcatacac tacggtcggc gcagtggtga tcagatccag atcgtattca cgttccagac
     1741 gttcctggat gatctccatg tgcagcaggc cgaggaagcc gcagcggaaa ccaaagccca
     1801 gcgcactgga gctttccggc tcatagaaca gtgaagcatc gttcaggctg agtttgccca
     1861 gcgcgtcacg gaaggcttca tagtcgtcgg aacttaccgg gaacagaccg gcgtatacct
     1921 gcggtttgac tttcttaaag ccaggcagcg ccttttctgc cggattacgc gccagcgtta
     1981 aggtatcgcc tactggagcg ccgtggatat ctttaatcgc acataccagc cagcctactt
     2041 cgccacattt cagttcagtg cggtcaacct gcttcggcgt gaagatgccc agacggtcgg
     2101 cgttataggt ctgcccggta ctcatgactt tcactttgtc gcccttacgc agggtgccgt
     2161 ttttaatacg gataagtgaa acaacgccca ggtagttgtc gaaccaggag tcgataatga
     2221 gtgcctgtaa cgggccttcc ggatcgcctt ccggcggcgg aatgtcgcgc accagacgtt
     2281 cgagaacgtc ctgcacgcca acgccggttt tcgctgaaca gcgcaccgcg tcggtggcat
     2341 cgatgccgac aatatcttca atttcttccg ccacgcgttc aggatcggct gccggcaggt
     2401 caatcttgtt cagtaccggc accacttcga gatccatttc catggcggtg tagcagtttg
     2461 ccagggtttg cgcttctacg ccctgcccgg cgtcgaccac cagcaatgca ccttcacagg
     2521 cagccagcga acgagaaact tcataagaga agtcaacgtg gcccggggtg tcgataaagt
     2581 taagctgata ggtttcgccg tcagacgctt tgtagtccag cgtcacgctt tgcgctttga
     2641 tggtaatgcc acgctcacgc tcaagatcca tggaatcgag aacctgcgcc tccatttcac
     2701 ggtcagacag gccaccgcag atctggataa tacggtcaga cagcgtcgat ttaccgtggt
     2761 caatgtgagc tatgatcgaa aagttacgta tattcttcat aaagtatgat tattgtgcct
     2821 taatgcccgg ttaaccaggc ttttagaagt cgctgttctg agcttaacgt ctgtattaat
     2881 agaaacgccg cattctacac tacaacattg aggcgaggaa atgttcatac cgtatggatt
     2941 gtggtatctg gaaacgtcct cgcatttgtt atgcaaaatg caacaaagcc agtgaaatca
     3001 ctggctcgcg tcttccgaag atgtttcaaa tcgcacaagg ccaggcggca aggccacgct
     3061 taagatgatc ggttgccatt cagaccgggc agcaaacttt cgagagcagc cacgtgcaat
     3121 caggaatcca ccaacgccac cgagaatcgc accgcataat gctgcaatat ctgaagcaaa
     3181 gagtagctga aatagtgaag cgatgaggaa tagtcccatc aacggcgaca tataaaccag
     3241 taatgcggag ctaagcaggc tgccttcggc gattcctaat tctacttttt gccccggcac
     3301 taacggctca tcacagggta cgacaatggt atgcgtggtt tgcgggccaa gtttattcaa
     3361 cacgcggcta ccgcaaccgg cgcgtgaagc gcagctgctg catgaggctt taacatcaca
     3421 actaaccagc gcctgcccgt tttcccagga gatgacggta gcccactctt tgatcattgc
     3481 gctgccccga acttaatatt ctcggcaatg cgtttcgccg tttgcggcgg cagttcaccg
     3541 acaatggtga tttcggcgtt atcacgtacg cttgtactga cggttctgcg tccggtgcgc
     3601 aacatctgat cggtgctcga tggcgtagcg cggttaacgt ttaccgaaaa gctgaataat
     3661 ccgtcggaat agagacgtga ttcgataggc atgttgtcca tcgtcggtag cggacgtcga
     3721 ctactggaaa cttcgctaaa accctgtggc aaccaggttg gcgtccagct gaatttagct
     3781 ttttcaccta caggaacaga aagcaacggc ggcaaatttg ccttcgccaa cgtctgcata
     3841 ctgctgctga tatcctgatt gacattaaaa gcaatcacgc gaaattgttc cagcgtttca
     3901 ccatcgcgat caaggagatc aacccgcatc ggtaatttcg attcggtgtc catccacacg
     3961 atgtagctgt agcgtgtacc atctcgggca accacacgaa tgacttcgca aagacgatca
     4021 gcaatacgcg tgcgcccgac ggagataaag tcgtagtaag gagaaaggcg tttgaaatcg
     4081 gtatagatga gcgatggtag agaatcaaca atgtaatcgc cattaagcgt gaacggttca
     4141 agtcccggtt caaaatagct gatttcattg ccgcgctgta ccacttcccg gcgcgggcca
     4201 tccatttgca acaattgtgc aagaggacgg ttatcgaggc gtgcatgtcg ataacgcaga
     4261 gactcaacac cctgtttatt gatgctgatg aatgacagct cgtaattcag tgactgactg
     4321 gccaggttca tctgctgtaa taacgccccg gacgcgggag tggccgaggc gttagcagag
     4381 aataacaggc tacctgtcac taatgacatg gcaaaccaaa gttgcttcat tactgcgatt
     4441 gcgttcctaa agtttgaatt cctggcacct gtacagcggc ttgctgagtt tgcgcctgct
     4501 caaactgaag ctgttcagag tggagtcggc gttgcagttc gtaatcctgc aacattgcat
     4561 taatgcgacg acgctgctcc tgtacctgct gctgttgacc attatttgcg gtcgcttcag
     4621 aaggtactcc caagctaacc gggctggctt tacccatcat cggcagtgta ttaaataccg
     4681 gcgtttcggg ctgctgggac gtttcagatt gtccattata gtgctggacc ccaacgataa
     4741 ctgcaagcga tacgcatgct gctacgccca tttgggtaag ctgtgccgcc cacggacgta
     4801 ctttctgcca gaatggcatt ttctgccatt gatgcggcgc aggctgggct tccgggatca
     4861 atgtcgccgg ttgacgtact ggctcttctt caatggcggc catcacgcgt gaagagatat
     4921 cgaaatggag cacctcggga gtatcacccc gcattgagtc acggattaag tgatagcttt
     4981 cccaggtttt ctgcatttct gggttatgag ccagttcgtt aagcagctca ctatccagcg
     5041 tttcgccatc cattaaagcg gaaagttgtt ctttctgcat gcctaatacc cttatccagt
     5101 atcccgctat cgtcaacgcc tgataagcgg ttgaactttg ttatcaatag cttccctcgc
     5161 tcggaagata cgtgaacgca ccgtacctac cggacaatcc atgatagcgg ctatctcttc
     5221 atagctcagg ccatccagct cccgcaaggt tattgccatg cgtaaatctt ccgggaggga
     5281 ctcaatagtt cggaaaacta tctgtctcag ttcttctgac aacattaagt tctcagggtt
     5341 cgaaatttct ttcaacgcgc cgccactttc gaagttttca gcttcaatgg catccacatc
     5401 actggaaggt ggacgacgcc cctgagcaac caggtaattt ttcgctgtat ttacagcaat
     5461 ccgatacagc catgtataaa aagcgctatc tccccggaac gaatccagcg cacgataggc
     5521 tttaataaaa gcttcttgta ccacatcggg aacatcaccc gacggcacat agcgggaaac
     5581 cagactcgcc actttatgct gatagcgcac taccagtaag ttaaaggctt tctgatctcc
     5641 cttctggacc cgttcaacca ggacctggtc cgttaactgc tcgctcatcc gaggtaaagt
     5701 ctccccaaac caaatttcca cgcgctatcg aaacgccact ccattagctg caatttgagc
     5761 aagcaaaggg ttagagtgtc tcgtttttgt aaagttccgt aacgcatctg tttttgtttg
     5821 tcatgctgta gacggatcat tatctatcat tataagtcta cagaatctga acatcgcatt
     5881 atctgtgtag aaatgcccat ttaactgcct gaagagtaac ccaacggcct ttttatttca
     5941 ccacctaatc ctccaccagc cagtaacttc tctttttctc gccgccctgc gtcagcgtgt
     6001 ttagccactg taacaaatat taaaatagca ggtgtttaat cgcacaacat gatgctatgc
     6061 tgaccaaacc gtgtttagta aattaaacaa agaaaatgaa tactctccct gatcattcat
     6121 gtgacgtgtt gattatcggt agcggcgcag ccggactttc actggcgctg cgcctggctg
     6181 atcagcatca ggtcatcgtt ctaagtaaag gcccggtaac ggaaggttca acattttatg
     6241 cccagggcgg tattgccgcc gtgtttgatg aaactgacag cattgactcg catgtggaag
     6301 acacattgat tgccgggggt ggtatttgcg atcgccatgc agttgaattt gtcgccagca
     6361 atgcacgatc ctgtgtgcaa tggctaatcg accagggggt gttgtttgat acccacattc
     6421 agccaaatgg cgcagaaagc taccatctga cccgtgaagg tggacatagt caccgtcgta
     6481 ttcttcatgc cgccgacgcc accggtagag aagtagaaac cacgctggtg agcaaggcgc
     6541 tgaaccatcc gaatatttgc gtgctggagc gcagcaacgc ggttgatcta attgtctctg
     6601 acaaaattgg cctgccgggc acgcgacgag ttgtaggcgc gtgggtatgg aaccgaaata
     6661 aagaaacggt tgaaacctgc cacgcaaaag cggtggtgct ggcaaccggc ggtgcgtcga
     6721 aggtttatca gtacaccacc aatccggata tttcttctgg cgatggcatt gctatggcgt
     6781 ggcgcgcagg ctgccgggtt gccaatctcg aatttaatca gttccaccct accgcgctgt
     6841 atcacccaca ggcacgcaat ttcctgttaa cagaagcact gcgcggcgaa ggcgcttatc
     6901 tcaagcgccc ggacggtacg cgttttatgc ccgattttga tgagcgcggc gaactggccc
     6961 cgcgcgatat tgtcgcccgc gccattgacc atgaaatgaa acgcctcggc gcggattgta
     7021 tgtatctcga catcagccat aagcctgccg attttattcg ccagcatttc ccgatgatct
     7081 atgaaaaatt gctcgggctg gggattgatc tcacacaaga accggtaccg attgtgcctg
     7141 ctgcacacta tacctgcggt ggtgtgatgg tggatgatca tgggcgtacg gacgtcgagg
     7201 gcttgtatgc cattggcgag gtgagttata ccggcttaca cggcgctaac cgcatggcct
     7261 cgaattcatt gctggagtgt ctggtctatg gctggtcggc ggcagaagat atcaccagac
     7321 gtatgcctta tgcccacggc gtcagtacgt taccaccgtg ggatgaaagc cgcgttgaga
     7381 accctgacga acgggtagta attcagcata actggcacga gctacgcctg tttatgtggg
     7441 attacgtcgg cattgtgcgc acaacgaagc gccttgaacg cgccctgcgg cggataacca
     7501 tgctccaaca agaaatagac gaatattacg cccatttccg cgtctcaaat aatttgctgg
     7561 agctgcgtaa tctggtacag gttgccgagt tgattgttcg ctgtgcaatg atgcgtaaag
     7621 agagtcgggg gttgcatttc acgctggatt atccggaact gctcacccat tccggtccgt
     7681 cgatcctttc ccccggcaat cattacataa acagataaaa agcctgggtc agcgccgtat
     7741 acgcttcgga atagttctgg tctggcccac gaatgactaa gcgatcgcta aagcattctc
     7801 ccgcctgcgg ggagaatgcc agcagcaccc gatgcggcag tcgcgcttcg ttttccgcca
     7861 catccgtccg caaacgtaaa tgccagccca tacttaatgc cagttcagta aaaccattac
     7921 caatctgctc tggcagcacc acgcagaaaa atccctcttc ggtaatgcac tccgccgcac
     7981 aggtcagcaa cgatgggtga tcaagcgtag tggtatagcg agcctgttcc cgttgaggtg
     8041 tcgagcactc tactccctgc tgatagtaag gtgggttact gatgattaaa tcgaagcgta
     8101 ctgtctgctg tgtgatccac tgctgaatat ccgccgtatg gacgttaatc cgctttgccc
     8161 acggggactg gttgatattt tcctgtgcct gcgcggcagc ttcactttcc agttcaactg
     8221 catcaatcat tacgctgtca tcggttcgct gcgccagcat taatgccagc aacccgctac
     8281 ccgcgccgat atcaaggcaa cgttttaccc cagccaccgg tgcccatgcg cccagcaaaa
     8341 tgccatccgt tcccactttc atcgcacaac gatcgtgagc aacaaaaaac tgtttaaaag
     8401 taaatccatt acgacgaagc acggatgtag actgtgacat gaaaataaaa ccttgcagga
     8461 aaaacggcga tagcaccggg tgagaacaat acccgagaag cgatatccat acaaacagat
     8521 gaagattgca gccgtaacgt ctataatcag cgccccacac agaggtagaa catgactgta
     8581 acgacttttt ccgaacttga actcgacgaa agcctgctgg aagccctcca ggataaaggt
     8641 ttcactcgcc cgaccgccat tcaggctgcc gccattccgc ctgcgctcga tggccgtgat
     8701 gtactcggtt ctgcgccgac aggcaccggt aaaacggcgg cgtatctgct gccagcgttg
     8761 cagcacctgc tcgatttccc acgtaagaaa tccggtccgc cgcgtatttt gatcctcacc
     8821 ccaactcgcg agctggcgat gcaggtggcc gatcatgccc gcgaactggc gaaacatacg
     8881 catctggata ttgccaccat caccggcggc gtagcctata tgaaccacgc ggaagtgttc
     8941 agcgaaaatc aggacatcgt ggtcgccacg accggacgtc tgctgcaata cataaaagaa
     9001 gagaacttcg attgccgcgc ggttgaaacg ctgatcctcg acgaagcaga ccgtatgctg
     9061 gatatgggct tcgctcagga tatcgaacat attgctggcg aaacgcgctg gcgtaaacag
     9121 accctgctct tttcggcaac tctggaaggc gatgcgattc aggactttgc cgagcgtctg
     9181 ctggaagatc cggtggaggt ttctgccaat ccgtccacac gtgagcgcaa aaaaattcat
     9241 cagtggtatt accgcgccga tgacctggaa cataaaaccg cattgctggt gcatctgtta
     9301 aaacagccgg aagcgacccg ctcaattgtg tttgtgcgta agcgtgagcg tgtgcatgag
     9361 ctggcaaact ggctgcgcga agcgggcatc aacaactgct atctcgaagg tgagatggta
     9421 cagggcaagc gtaacgaagc gatcaagcgt ttgaccgaag gtcgcgtaaa cgtactggtt
     9481 gccaccgatg ttgccgcgcg cggtatcgac attcctgacg tcagccacgt ctttaacttc
     9541 gatatgccgc gcagtggcga tacttatttg caccgtatcg gacgtaccgc gcgcgccggt
     9601 cgtaaaggca ccgcaatttc gctggtagaa gcccatgacc atctgctgct gggcaaagtt
     9661 ggtcgctata ttgaagagcc aattaaagct cgtgtaattg atgagttacg cccgaaaacg
     9721 cgtgcgccaa gcgagaagca gaccggcaag ccatcgaaga aagtactggc aaaacgcgcc
     9781 gagaagaaaa aagctaaaga gaaagagaag ccgcgggtga aaaaacgcca tcgcgacacc
     9841 aaaaatattg gtaagcgccg taaaccgagc ggaacgggcg tgccaccgca aacgacagaa
     9901 gagtaatctc aatgccgggt ttaagcccgg cattattttt atcccggcaa aaaacactct
     9961 tcaacacact gaataaaaat gtgcatcgcc gggctaaccg cttttccggc atggtgagcg
    10021 cacattgccg taatggtctg cgactgttcg ccaaaaggca attcgattaa ttcgccactc
    10081 gcaagttcat ttgccactgt aaagcgcggc aggtaactga ccccgatatt tgccgcgaca
    10141 caacgcttga tgctttcgat acttataagc tcaatggtgt tttccaccgt gatccgtcgc
    10201 tggcgcagcg tactttcaaa tagctggcga aagacacatt gcggttcatt gataataaaa
    10261 ctacaggcgt tatgtcttcc cggctcagta aaatcgacat ctttaatttg cggtgaagcc
    10321 accagcacca gtgattgttc acccaactct cgtcggttca gagcatcatc attccctacg
    10381 cggtaaaaga cgccgacatc agcctcatca ttcagtagcg catcacggat cacgtaacag
    10441 ttcagagact gcaacgataa acgcactttt ggtgcgcgtt gccgaaaacg ctgcaacacc
    10501 tgcggcattc ggtaagaaag caacgtttcg cctgaaacaa cgcgtagttc cccgtccgga
    10561 tccgactctt ttttggcagc ctcacgaagg gtatccatca ctctggtgag ttcatagatg
    10621 tgcggcagca gcttttttcc ttcgcgggta aggcacattc gtcgaccaat tttctcaaat
    10681 aactggactg agaactcctg ctcaagctgc tgaatatgaa aagtcaccgt cgattgtgtg
    10741 cagcacaatt tttgcgaagc tcgcaaaaag gacccctctt ccaccacggt tttaagcgta
    10801 ataaaacggc gcagatccat aaccccaaac ctatcgaaaa tatcgaattt agaatgtaaa
    10861 aacattcatt tttttaaatg ttccgtgtcg ggtactgtct accaaaacag aggagataac
    10921 aagtgacacc gaccctttta agtgcttttt ggacttacac cctgattacc gccatgacgc
    10981 caggaccgaa caatattctc gcccttagct cagctacaac gcacggattt cgtcagagta
    11041 ctcgcgtgct ggcagggatg agtctgggat ttttaatcgt catgttgcta tgcgtgggaa
    11101 tttcgttttc actggctgtt atcgacccgg cagcagtgca tctgttgagc tgggcaggag
    11161 cggcatatat tgtctggctg gcatggaaaa tcgctaccag tccaacaaag gaagacggac
    11221 ttcaggcaaa accaatcagc ttttgggcca gctttgcttt gcagtttgtg aacgtcaaaa
    11281 tcattttgta tggcgttacg gcactgtcga cgtttgtcct gccgcaaacg caggcgctaa
    11341 gctgggtagt tggcgtcagc gttttgctgg cgatgattgg gacgtttggc aatgtgtgct
    11401 gggcgctggc ggggcatctg tttcagcgat tgtttcgcca gtatggtcgc ccgttaaata
    11461 tcgtgctggc gctgttgctg gtctattgtg cggtacgcat tttctattga aagacaaaaa
    11521 gaaaagcgga agaggtcacc ctcttccgct taataacttg ctacttaagc cttacaggct
    11581 ttcagtaaag gtacgagcga taacgtcgcg ctgctgttcc ggagtcagag agttaaagcg
    11641 aactgcataa ccggatacac ggatggtcag ctgcggatat ttttccggat gcttaactgc
    11701 atcttccaga gtttcacgac gcagaacgtt aacgttcagg tgttgaccac cttcaacgcg
    11761 aacttctggt ttcacttcta ctggaacttc acggtattca atatcaccca gtttgcttac
    11821 tgcaaccact tcgtcttctg caaaacctgc tttcgcaacg atgcaacgcg cttcgccttt
    11881 ttcgctgtcc agcagccaga aagagttcag cagatcgtcg ttagcggctt tagtaatctg
    11941 gatacctgta atcatgtgat gcctccccgg caaaattatt tgatttgttc agcctgtcgc
    12001 ggccaattgg taaaaccatt gttgcttgag tgtatatata ctcctcaaac acccttgaat
    12061 ctttgattta aatcaataaa aaccacacat caagtatggt tgcaaatgga ttttattgtt
    12121 ttacatcaac ttatgcaggt gtgaaatttt accaatttac atttttttgc actcgtttaa
    12181 gtctaaaaaa tgagcatgat tttgttctgt agaaagaagc agttaagcta ggcggattga
    12241 agattcgcgg gagagcgaga tggcaaacga attaacctgg catgacgtgc tggctgaaga
    12301 gaagcaacaa ccctattttc ttaataccct acagaccgtc gccagcgagc ggcagtccgg
    12361 cgtcactatc tatccgccac aaaaggatgt ctttaacgcg ttccgcttta cagagttggg
    12421 tgacgttaaa gtggtgattc tcggccagga tccttatcac ggaccgggac aggcgcatgg
    12481 tctggcattt tccgttcgcc ccggcattgc gacaccgccg tcactgctga atatgtataa
    12541 agagctggaa aatactattc ccggtttcac ccgccctaat catggttatc ttgaaagctg
    12601 ggcgcgtcag ggcgttctgc tactcaatac tgtgttgacg gttcgcgcag gtcaggcgca
    12661 ttcccacgcc agcctcggct gggagacctt caccgataaa gtcatcagcc tgattaacca
    12721 gcatcgcgaa ggcgtggtgt ttttgttgtg gggctcacat gcgcaaaaga aaggggcgat
    12781 tattgataag caacgccatc acgtactgaa agcgccgcat ccatcaccgc tttctgcgca
    12841 tcgtgggttc tttggctgca accattttgt gctggcaaat cagtggctgg aacaacgtgg
    12901 cgagaaaccg attgactgga tgccagtatt accggcagag agtgagtaaa tttgcaggaa
    12961 aacgccggat ggcagcgttg ccacccggct gatttatcag gctttattct gacgccacca
    13021 ttcaccaagc aaaacgccag ttgcgacaga gatatttagc ccggcaacgt tgccagtacc
    13081 gtcaatcttc acgcgcagat cgtttggatc gcgtgcggca tccggtaacc cttcatattc
    13141 ctgacccagc accagtacca ttttcgctgg cagactggtt ttgaacagcg gtttcccctg
    13201 ctcgctggaa gtggtcacta cggtgtaacc tgcctgacgg aaatcatcca gcacgttaac
    13261 aatgttgtcg ccagtaatcg gctgaacgtg ctctgcgccg ccttctgcgg tacggatagc
    13321 cgcccccgac tccagcagtg cctcatcctg caccacaacg cctttcacgc cgaagtgcgc
    13381 acagctgcgc atcatgccac ccaggttatg cgggttagat tcgttttcca gtgccagaac
    13441 gcaatcctgc gcgcctgcct ggcttaccca ctgctgcacg gttgtaccgt tacgcttttt
    13501 gatcaggaag caaacgccgc cgtgatgttc cgtgcctgac gcttttgtca gttccgcttc
    13561 atctaccaca tggtacgctt tgcggtttgc tgccatccag cgcaaggctt ctttaaaacg
    13621 cggcgttaca ctctggataa accaggcgcg aacaatcgct tccggacggc tctggaacag
    13681 tgcctgacag gcattttcgc cgtagacgcg ggtttcttcc gcacgctgac gacgcaaaac
    13741 ttccggatca ataaaacttt taccactgat gccaccgtga tcggcctttt ccggcgtctc
    13801 atcacccggc gcgcgggaaa ccgtgcgcca cggcgaatct tcccacttgc ggtcacgggg
    13861 gtgactctgt ttgtcatcgc gggcggggcg acggccaccg tcagcacgag attttcctgg
    13921 acgcccgccc cctttcccgg tacgcgggtt gtgggtacgt ttatcagaat catcatcact
    13981 gcggacatac atcactttga ccttgccgct tttacctttc atttcatcgt tcatgctttt
    14041 ctccaccagc gctgcgcgaa gcgcgcagat tacccgaagt ccgcgcggtt cgccatgatt
    14101 tcgtaccaaa gcctgcgact atcataccta ttgaataaaa cagattgttg tctggaacaa
    14161 tgtccccgat aatatgtaac atattagaaa cataccggcg tcgttgccga taagtctcct
    14221 tactcatccc gaggttagtt atgaataccg tttgtaccca ttgtcaggcc atcaatcgca
    14281 ttcccgacga tcggatcgaa gatgcggcaa aatgcggacg ctgcggtcac gacttgtttg
    14341 acggagaggt gattaatgcg accggtgaaa cgctcgacaa attgctgaaa gatgatctac
    14401 ctgtggtgat cgacttctgg gcaccgtggt gcggcccctg ccgtaatttc gcaccaattt
    14461 ttgaagatgt cgcgcaagag cgtagcggta aagtgcgctt tgtgaaagtg aataccgaag
    14521 ctgaacgtga attgagcagt cgctttggaa ttcgtagtat accgacgatc atgattttca
    14581 aaaacggtca ggttgtcgac atgcttaatg gcgcagtacc gaaagcgccg tttgatagct
    14641 ggctgaacga atctctttaa tcttaccggg gcgcatcttg tgccccgttt tctcctctgc
    14701 gacaatggcg ttttttcgac gctctcttat gaccgaaaac gctgttctcc agttacgcgc
    14761 cgagcgtatt gcgcgcgcaa cacgtccttt tcttgcccgc ggtaatcgcg ttcgtcgctg
    14821 ccaacgctgt cttttgccag agaaattatg tctctgttcg acaattaccc cagcacaagc
    14881 aaaaagtcgc ttctgtttgc tgatgttcga caccgagcca atgaagccca gtaataccgg
    14941 gcgtctgatt gctgatattt tgcctgatac cgttgcgttt caatggtcgc gtaccgaacc
    15001 ctcgctagat ttgctggatc tggtacaaaa cccggactat cagccaatgg tggtctttcc
    15061 cgcctcgtat gctgatgagc aacgggaagt gatcttcaca ccacctgccg gtaagcctcc
    15121 gctatttatc atgctcgatg gtacctggcc tgaagctcgc aagatgtttc gtaaaagtcc
    15181 gtatctggat aatcttcccg tcatttccgt cgatctttcc cggctttctg cctatcgcct
    15241 gcgtgaagcc caggctgaag gccaatattg tactgccgag gtagccatcg cactgttaga
    15301 tatggccggc gataccgggg cggcggcagg gttaggcgag cattttaccc gctttaaaac
    15361 acgctatctg gcaggaaaaa cgcaacatct gggtagcatc acagcagaac agttagaaag
    15421 cgtttaaaat cattcggtca cttctgcggg agaccggtat gagtcagcga ggactggaag
    15481 cactactgcg accaaaatcg attgcggtaa ttggcgcatc gatgaaaccc aatcgcgcag
    15541 gttacctgat gatgcgtaac ctgctggcgg gaggctttaa cggaccggta ctcccggtga
    15601 cgcctgcctg gaaagcggtg ttgggtgtgt tggcctggcc ggatattgcc agcttgccct
    15661 ttacacccga ccttgcggtt ttatgtacca atgccagccg taatcttgct cttctggaag
    15721 agctcggcga gaaaggctgt aaaacctgca ttattctttc cgccccggca tcgcaacacg
    15781 aagatctccg cgcctgcgcc ctgcgccata acatgcgcct gctaggacca aacagtctgg
    15841 gtttactggc tccctggcaa ggtctaaatg ccagcttttc acctgtgccg attaaacgcg
    15901 gcaaactggc gtttatttcg caatcggctg ccgtctccaa caccatcctc gactgggcgc
    15961 aacagcgtga gatgggcttt tcctacttta ttgcgctcgg cgacagcctg gatatcgacg
    16021 ttgatgaatt gctcgactat ctggcgcgcg acagtaaaac cagcgccatc ctgctctatc
    16081 tcgaacagtt aagcgacgcg cgacgctttg tttcggcggc ccgtagtgcc tctcgcaata
    16141 aaccgattct ggtgattaaa agcggacgta gcccggcggc tcaacgtctg ttgaacacca
    16201 cggcaggaat ggatccggcg tgggatgcgg ctattcagcg tgccggtttg ttgcgggtac
    16261 aggatactca cgagctgttt tcggcagtgg aaaccctcag ccatatgcgc ccactgcgtg
    16321 gcgaccggct gatgattatc agcaacggtg ctgcgcctgc cgcgctggcg ctggatgcct
    16381 tatggtcacg caatggcaag ctggcaacgc taagcgaaga aacctgccag aaactgcgcg
    16441 atgcactgcc agaacatgtg gcaatctcta acccgctcga tctacgcgat gacgccagca
    16501 gcgagcatta tgtcaaaacg ctggacattc tgctccacag ccaggatttt gatgcgctga
    16561 tggttattca ttcgcccagc gccgctgctc ccgcaacaga aagcgcgcaa gtattaattg
    16621 aagcggtaaa gcatcatccc cgcagcaagt atgtttctct gctgacgaac tggtgtggcg
    16681 aacattcctc gcaagaggca cgacgattat tcagcgaagc cgggctgccg acctaccgta
    16741 ccccggaagg aaccatcact gcttttatgc atatggtgga gtaccggcgt aatcagaagc
    16801 aactacgcga aacgccggcg ttgcccagca atctgacctc caataccgca gaagcgcatc
    16861 ttctgttgca acaggcgatt gccgaaggag caacgtcact cgatacccat gaagttcagc
    16921 ccatcctgca agcatatggc atgaacacgc tccccacctg gattgccagc gatagcaccg
    16981 aagcggtgca tattgccgaa cagattggtt atccggtggc gctgaaattg cgttcgccgg
    17041 atattccaca taaatcggaa gttcagggcg taatgctcta cctgcgtaca gctaatgaag
    17101 tccagcaagc ggcaaacgct attttcgatc gcgtaaaaat ggcctggccg caggcgcgga
    17161 tccacggcct gttggtgcaa agtatggcta accgtgctgg cgctcaggag ttgcgggttg
    17221 tggttgagca cgatccggtt ttcgggccgt tgatcatgct gggcgaaggc ggtgtggagt
    17281 ggcgtcctga agatcaagcc gtcgtcgcac taccgccgct gaacatgaac ctggcccgct
    17341 atctggttat tcaggggatc aaaagtaaaa agattcgtgc gcgcagtgcg ctacgcccat
    17401 tagatgttgc aggcttgagc cagcttctgg tgcaggtttc taacttgatt gtcgattgcc
    17461 cggaaattca gcgtctggat attcatcctt tgctggcttc tggcagtgaa tttaccgcgc
    17521 tggatgtcac gctggatatc gcgccgtttg aaggcgataa cgagagtcgg ctggcagtgc
    17581 gcccttatcc gcatcagctg gaagaatggg tagaattgaa aaacggtgaa cgctgcttgt
    17641 tccgcccgat tttgccagaa gatgagccac aacttcagca attcatttcg cgagtcacca
    17701 aagaagatct ttattaccgc tactttagcg agatcaacga atttacccat gaagatttag
    17761 ccaacatgac gcagatcgac tacgatcggg aaatggcatt tgtagcggta cgacgtattg
    17821 atcaaacgga agagatcctt ggcgtcacgc gtgcgatctc cgaccctgat aacatcgatg
    17881 ccgaatttgc cgtgctggtt cgctcggatc tcaaagggtt aggcttaggt cgacgcttaa
    17941 tggaaaagtt gattacctat acgcgagatc acggactgca acgtctgaat ggtattacga
    18001 tgccaaacaa tcgtggcatg gtggcgctgg cccgcaagct cgggtttaac gttgatatcc
    18061 agctcgaaga ggggatcgtt gggcttacgc taaatcttgc ccagcgcgag gaatcatgag
    18121 taaggtactg gaaatgttga ccacttaatc gggactggtg ttattattgc ccgcttatgt
    18181 cgtctgcatt gcacagagga cccttcaatg aacagagaag aaatgcactg tgatgttgtc
    18241 aaaatttaag cgtaataaac atcaacaaca ccttgcccaa ctacccaaga tttctcaatc
    18301 agttgatgat gtcgatttct tttacgctcc cgccgacttc cgggagacgc tgctggaaaa
    18361 aatagccagc gcgaagcagc gcatttgcat tgtcgccctg tatctcgaac aggatgacgg
    18421 tggcaaaggc attctgaacg cgttgtatga ggctaaaagg cagcgtccgg aactggatgt
    18481 gcgggtgctg gtcgactggc atcgtgcaca acgtggacgc attggcgctg cggcatctaa
    18541 cactaacgct gactggtact gccgcatggc gcaggaaaat ccgggcgtag atgttccggt
    18601 ttatggcgtt ccaatcaata cccgtgaagc ccttggtgtt ctgcacttta aaggctttat
    18661 catcgacgat agcgtacttt atagcggtgc cagcctgaac gatgtttacc tgcatcagca
    18721 cgataaatat cgctacgacc gttatcatct tatccgtaac cgtaagatgt cagacattat
    18781 gtttgaatgg gttacacaga atattatgaa tggccgcggc gttaatcgtc tggatgatgt
    18841 taatcggcca aaaagcccgg aaatcaagaa cgatattcgt ctgttccgcc aggagctgcg
    18901 tgatgccgct tatcatttcc agggcgatgc cgacaacgat cagctttccg taacgccgtt
    18961 ggtaggtctg gggaaatcga gtctgttgaa caagaccatt ttccatctta tgccttgtgc
    19021 cgagcagaaa ctaaccatct gcacacctta cttcaacctg ccagcaatcc ttgtgcgcaa
    19081 tattatccag ttgctgcgcg aagggaaaaa ggtcgaaatt attgttggtg ataaaaccgc
    19141 gaatgacttc tacattccgg aagatgaacc tttcaagata attggcgcat tgccttatct
    19201 ctatgagatc aatctgcgtc gtttcctgag ccgtttgcag tattacgtca acactgacca
    19261 gttagtggtt cggttatgga aagatgacga caacacctat cacctgaaag ggatgtgggt
    19321 tgatgataag tggatgttga tcactggtaa taacctgaac ccgcgcgcct ggcgtctgga
    19381 tctggaaaac gctattttga tccacgatcc gcaactggag cttgcgccac agcgagagaa
    19441 agaactggag ctgatccgcg agcataccac catcgttaag cactatcgcg acctgcaaag
    19501 tattgccgat tatccggtga aggttcgtaa actcatccgt cgtttgcgcc gtatccgcat
    19561 cgaccgatta attagccgca tcctgtaatc acaaccccgt cctgtacggg gtttgttttt
    19621 tggaggccac gttttgcgta ttctttttgt ctgttcgcta ttattacttt ctggatgcag
    19681 ccatatggct aacgatagct ggagcgggca ggataaagct caacacttta tcgcctcggc
    19741 gatgctttcc gccgccggaa atgaatatgc acagcatcag gggatgagtc gcgatcgcag
    19801 tgccactttc gggctgatgt tctctgttgg gctgggggcg tcaaaagaac tttgggatag
    19861 ccgccccgag gggagcggct ggagctggaa agattttacc tgggatgtcg ccggtgcaac
    19921 tactggctat accgtctggc aactgacccg tcactaaaga cgcatcccct tccctttgcg
    19981 atgtagcatc aaagaaacca gaaacgccac cacggccatc aaggtcacat accagaagaa
    20041 ggctgtttcc attcctattg atttcagcga caacgctacg tactccgccg aaccaccaaa
    20101 tatagcattg gcgaccgcat atgacagacc aacacctaat gcacgaacct gtgccgggaa
    20161 catctcagcc ttcagtattc cactgattga agtataaaaa ctcacgatca gcagggcaca
    20221 catcaccaga ccaaaagcgg cataaggcga ggaaacgttt tgcaatgctg agagaatagg
    20281 aacggtaaaa atggctgcca gagaaccgaa acataacatt gaggtacgac gaccaatctt
    20341 atccgacagc gcgccaatga gtggttgaat aagcatgaat acaaacaatg cggcagtcat
    20401 aatgccactc gccacgttgg catgcattcc cgcagtattt accagatact tctgcatata
    20461 agtggtgaag gtatagaaac aaagggagcc cgcagcggta aaaccgagaa ccatgatgaa
    20521 tgcacggcga ttgcgccata atcctttcag agatccagct tcttttaaag cgcgtgtttc
    20581 ttgttgtgaa gtttcatcta actgacgacg taaccacaac gccacaacag ctaacacagc
    20641 tcctaacgcg aaaggaatac gccatcccca ctctctgagt gcagcgtctt ccatggtgtg
    20701 ttgtaaaacc acgacaacca gtagagctag cagttgcccg ccgatcaacg tcacatactg
    20761 aaatgatgcg taaaaacctt tgcgcccttc aacggcaact tcactcatat aggtggcgct
    20821 ggtgccatat tctccgccaa cggataatcc ctggaataaa cgagcaagaa gcaataatgc
    20881 cggagcccag gtaccgattg tttcgtagcc tggcaaacag gcgataacca gtgagccgaa
    20941 acacatcata cacaccgata acagcatcga ttttttgcga ccatgtttat cggctatgcg
    21001 gccaaatagc caaccgccta ttgggcgcat cagaaatccc gcagcaaaaa cacctgctgt
    21061 ttgcagtagt tgagtcgtcg tgttcccgga agggaagaag atgtgagcga agtagagtga
    21121 acagaacgag tagacataga aatcgaacca ctcgaccaga ttacctgaag aggcccccac
    21181 aattgcccaa atgcggcgac gagtatcact acttgtcagt ttgctgtctg ccgttacagt
    21241 actttcagcc atgccattat gtctcctgcc gtaatccgat gcttttgtcg gtcgcttttg
    21301 tttatttctt ttggtaaaga aaatgttata catttgttgc atatcatcat gcaaccttaa
    21361 ccacgaattt agttagcagg aaaatggtta ttgaggagct taaggataaa tttctggtaa
    21421 ggaggacacg tatggaggtg ggcaagttgg ggaagccgta ttcgctggcg tatgtggagg
    21481 aataagctgc cccctatcgt gcctgcgtaa gctggataag acgcgccatg aactgaaccc
    21541 caaaacctgg acagtttcag ttaaccggct ttttgatact cagtccggta ttctaccggg
    21601 ctgagtcccc caagactgag ttttattctt ttttgattcc agtaagttat gtattcgtta
    21661 acggcatttt ccagctcttc taca
//
LOCUS       DAOTRK010000078        21411 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.078, whole genome shotgun sequence.
ACCESSION   DAOTRK010000078 DAOTRK010000000
VERSION     DAOTRK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.078"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            66..6173
                     /gene="irp2"
                     /locus_tag="QIE41_002794"
     CDS             66..6173
                     /gene="irp2"
                     /locus_tag="QIE41_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HDS3531667.1"
                     /translation="MISGAPSQDSLVPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLATHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            6261..15752
                     /gene="irp1"
                     /locus_tag="QIE41_002795"
     CDS             6261..15752
                     /gene="irp1"
                     /locus_tag="QIE41_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HDS3531668.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLKDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDRRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTVPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDGGRKSTALLLSAASDSALRRLATDYAGALRKNADASDLAFTALHAR
                     RLDLPFRLAVPLNRETADALSTWASEKSGAQVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAVVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNSA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFNHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLANALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTVGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            15749..16849
                     /gene="ybtU"
                     /locus_tag="QIE41_002796"
     CDS             15749..16849
                     /gene="ybtU"
                     /locus_tag="QIE41_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HDS3531669.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLMRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            16846..17649
                     /gene="ybtT"
                     /locus_tag="QIE41_002797"
     CDS             16846..17649
                     /gene="ybtT"
                     /locus_tag="QIE41_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HDS3531670.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            17653..19230
                     /gene="ybtE"
                     /locus_tag="QIE41_002798"
     CDS             17653..19230
                     /gene="ybtE"
                     /locus_tag="QIE41_002798"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HDS3531671.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNTQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            19361..21382
                     /gene="fyuA"
                     /locus_tag="QIE41_002799"
     CDS             19361..21382
                     /gene="fyuA"
                     /locus_tag="QIE41_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HDS3531672.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRAFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         4290 a         6554 c         6212 g         4355 t
ORIGIN      
        1 ttgttaataa ttattattct catattagta atgcttttcg gtaagacgtg ccatcaggag
       61 gaagaatgat ttctggcgca ccatctcagg attcgctggt accggacaac cgccacgcgg
      121 ctgattacca acaattacgc gagcggctca tacaggaact gaatttaacg ccgcagcagt
      181 tacatgaaga gagcaacctg atccaggccg gcctggattc cataagattg atgagatggt
      241 tacactggtt tcgtaaaaat ggctaccgcc ttacccttcg cgagctgtat gccgccccca
      301 cgctggcggc atggaaccag ttaatgctca gccggtcgcc ggagaacgcg gaagaagaaa
      361 cgccgcccga cgaatcatcc tggccgaaca tgaccgaaag tacgcccttc ccattgacgc
      421 cagtacagca cgcctacctg acgggccgca tgccggggca gacgcttggc ggcgtgggtt
      481 gccacctgta tcaggagttt gaaggccatt gtctgacggc gtcgcagctg gagcaggcca
      541 tcacgacctt gctgcaacgc cacccaatgc tgcatatcgc ctttcgcccc gacgggcagc
      601 aggtctggct accgcaacct tactggaacg gcgtcaccgt tcatgattta cgccataacg
      661 acgctgaaag ccgccaggcc tatctggacg cactgcgcca gcgcctgagc caccgtcttt
      721 tacgcgtgga aatcggcgaa acgtttgatt ttcagctgac gctcttgccg gacaatcgcc
      781 accgcctcca tgtcaatatt gacctgctga ttatggatgc ctccagcttt acgcttttct
      841 tcgatgagct taacgccctg ctggccggag aatcgctgcc ggctatcgac acccgctatg
      901 atttccgctc gtatttgctg caccagcaga agatcaatca accactgaga gacgacgcgc
      961 gcgcttactg gctggcgaaa gcatcgacgc ttccccccgc gcccgtcttg ccgctggcct
     1021 gcgaacccgc cacgctacgt gaagtccgta atacccgacg ccgcatgatt gtcccggcaa
     1081 cacgctggca cgcctttagc aaccgggccg gcgagtatgg cgtgacgccg acaatggcac
     1141 tggcgacctg tttttctgcc gtgctggctc gctggggcgg cctgacgcgt ctgctgctta
     1201 acatcacctt attcgaccgc cagccgctgc acccggcggt tggcgcgatg cttgccgact
     1261 tcaccaatat tcttctgctg gataccgcct gcgatggcga taccgtcagc aacctggcgc
     1321 gtaaaaacca gctcacgttt acggaggact gggagcatcg ccactggtcc ggcgtcgaat
     1381 tactccgtga actcaaacgc cagcagcgct acccccacgg cgccccggtg gtatttacca
     1441 gcaatctggg gcgttccctc tacagcagcc gcgcagaatc gccgttgggc gagccggaat
     1501 ggggcatctc gcaaacgccg caggtctgga tagatcatct ggcgttcgag catcacggcg
     1561 aggtctggct acaatgggac agcaacgacg cgctgttccc tccggcgtta gtcgaaacat
     1621 tgttcgacgc ctactgccag ttgattaacc aactctgcga tgacgaaagc gcctggcaaa
     1681 agccgttcgc agatatgatg cccgccagcc agcgcgcgat acgcgaacgg gtcaacgcca
     1741 ccggcgcccc cattcccgaa ggcttgctgc atgaaggcat tttccgtatc gctctgcaac
     1801 agccgcaggc gctggcggta acggacatgc gttatcagtg gaattatcat gagctgacag
     1861 actatgcccg ccgttgcgcg ggcaggttaa tcgagtgcgg ggttcagccc ggcgataatg
     1921 tggctatcac gatgtcgaaa ggcgcaggac aacttgttgc ggttctggcc gtcctgctgg
     1981 ccggggcggt ttacgttccg gtttcgctgg atcagcctgc cgcacggcgc gagaaaatct
     2041 acgctgacgc cagcgtccgg ctggtgctca tttgtcagca cgacgccagc gccgggtcag
     2101 acgatattcc cgtccttgcc tggcagcagg ccattgaggc ggagccgatc gccaacccgg
     2161 tagtacgcgc ccccacgcaa ccggcctaca ttatctacac ctccggctct accggtacgc
     2221 cgaaaggggt agtcatttct caccggggag cgcttaacac ctgttgcgat atcaataccc
     2281 gctatcaggt tggcccgcat gacagggtgc tggccctctc cgccctacat tttgatttat
     2341 cggtttacga catttttggc gtactgcgcg cgggcggcgc gctggtgatg gtgatggaaa
     2401 atcaacggcg cgatcctcac gcatggtgtg agctgatcca gcgccatcag gtcacgctct
     2461 ggaacagcgt cccggcgctg ttcgatatgc tgctgacctg gtgtgaaggt ttcgccgacg
     2521 ccacgccgga aaacctgcgc gcagtgatgc tttccggcga ctggatcggg cttgacctcc
     2581 ccgcccgtta tcgggccttc cggccacaag gacaatttat cgcgatgggc ggcgccaccg
     2641 aggcgtctat ctggtctaac gcctgcgaaa ttcacgacgt ccccgcccac tggcgctcca
     2701 tcccttacgg ttttccgcta accaaccaac gctaccgggt ggtggatgaa cagggccggg
     2761 actgccctga ctgggtgccg ggtgaattat ggattggcgg cattggggtc gcggaaggct
     2821 atttcaacga tcccctgcgt agcgagcagc aatttttgac gctcccggac gagcgctggt
     2881 atcgcaccgg cgatctcggc tgctactggc cagatggcac aatcgagttc ctcggtcgtc
     2941 gcgacaagca ggtcaaagtc ggaggatatc gcatcgagct gggcgaaatc gaaagcgcgc
     3001 tcagccagct ggcgggggtg aaacaagcaa ccgttctggc gatcggcgaa aaagaaaaaa
     3061 cgctggcggc atacgttgtt cctcagggcg aggctttttg cgttaccgat catcggaacc
     3121 cggcactgcc gcaggcgtgg cacacgcttg cgggaacgtt gccctgttgc gccatctcgc
     3181 cagagatctc cgcagaacag gtagccgatt tccttcagca tcgcctgcta aaactgaagc
     3241 cgggtcacac cgctggcgcc gatcctctcc ccctgatgaa ctcactcgct atccagccgc
     3301 gctggcaggc cgtggtggaa cgctggttag catttctggt gacacaacgg cgactgaagc
     3361 ccgctgctga aggttatcag gtctgcgctg gtgaagaacg cgaggatgag cacccgcact
     3421 tcagcggaca tgatttaacg ttatcgcaaa ttcttcgcgg tgcccgtaac gaactgtcgt
     3481 tactgaacga cgcgcagtgg tcgccggaaa gcctggcctt taaccatccg gccagcgccc
     3541 cgtatattca ggaactggcg acaatttgcc aacagcttgc acagcgctta cagcgcccgg
     3601 tacgcctgct tgaggtggga acccgcactg gccgcgccgc agaatcgctg ttagcacagc
     3661 tcaacgccgg acagattgag tatgtcgggc ttgagcagag ccaggagatg ctgctgagcg
     3721 cccggcagag gctcgccccc tggcctggcg cccgtctgtc cctctggaat gcagacacgc
     3781 tggcgacgca cgctcactcg gcggacatta tctggcttaa taacgccctg catcgtctgc
     3841 tgccggaaga tcccgggctc cttgcgacat tacaacagct tgccgttccc ggcgcgctgc
     3901 tctacgtgat ggagtttcgc cagttaacgc cgtccgccct actcagcacg ctcctgttaa
     3961 ccaatgggca gccggaggcc ttgctgcata acagcgccga ctgggcggca ttatttagcg
     4021 cggccggctt caactgtcag catggcgatg aggtcgcggg gttacaacgc ttcctcgtac
     4081 aatgtcctga caggcaggtg cgccgcgatc cccgtcaact tcaggccgcc ctcgccgggc
     4141 gtctgccggg gtggatggtg ccgcaacgga tcgtattcct cgacgcctta ccgctgacgg
     4201 ctaacgggaa aattgactac caggcgctga agcgtcgtca tacccctgaa gcggaaaacc
     4261 cggccgaagc ggatttaccc cagggcgaca ttgaaaaaca ggttgccgcc ctctggcagc
     4321 aactcttatc aactggcaat gtcaccagag aaaccgactt cttccagcaa ggcggcgata
     4381 gcctgctggc gacccgtctg accgggcaac ttcatcaggc aggttatgaa gcgcaattaa
     4441 gcgacctgtt taatcatccc cggctggcgg attttgccgc cacgctgcgg aaaaccgacg
     4501 tcccggtcga acaaccattc gtccactccc ctgaagatcg ctaccagccc tttgcgctta
     4561 ccgacgtgca gcaggcttac ctggtggggc gtcagccggg ctttgccctg ggcggcgtcg
     4621 gctcacattt ctttgttgaa tttgaaattg ccgatctgga cctcacccgg ctggagacgg
     4681 tctggaaccg attaatcgcc cgccacgata tgctgcgcgc catcgtgcgt gatggacagc
     4741 aacaggtgct cgaacagacg cccccttggg tgatacccgc acacaccctc catacgcctg
     4801 aagaggcgtt gcgggtgcgc gaaaaactgg cgcatcaggt actcaacccc gaagtgtggc
     4861 cggtattcga tctccaggtc ggatacgtgg acgggatgcc tgcccgcctg tggctgtgtc
     4921 tggataacct gttgcttgac ggtctgagca tgcagatcct gctggcggag ctggagcacg
     4981 gctaccgcta cccgcaacag ctgcttccgc cgctgcccgt caccttcagg gattatctgc
     5041 aacaaccctc gctacagtcg cccaatccag attctctggc atggtggcag gcgcagcttg
     5101 atgatattcc tccggcgcct gcgttgccgc tgcgctgctt gcctcaggag gttgaaacac
     5161 cgcgcttcgc ccgcctgaac ggcgcactgg acagcacgcg ctggcatcgg ctgaaaaaac
     5221 gggcggctga cgcccatctc accccgtcgg ccgtactgtt gtcggtgtgg tcaacggttc
     5281 tctctgcatg gagtgcacag cctgagttca cgcttaacct tacgcttttc gacaggcgac
     5341 cgctgcaccc gcaaatcaac cagattctgg gcgatttcac ctcgctgatg ctgctgagct
     5401 ggcatcccgg cgaaagctgg ctgcacagcg cgcagtcact acagcagcgg ctgagccaga
     5461 acctcaacca ccgcgatgtg tcagccatcc gcgtgatgcg tcaactggcg caacggcaaa
     5521 acgtgcctgc cgttccgatg cccgtcgtct ttaccagcgc actgggcttt gagcaggata
     5581 acttcctcgc ccggcgtaat ctgctcaaac cggtctgggg catctcccag acgccgcagg
     5641 tctggctcga tcaccagatt tatgaatccg aaggcgaact gcgctttaac tgggattttg
     5701 tcgccgcgct gtttcctgcc gggcaggtgg agcgccagtt tgaacagtat tgcgcattgc
     5761 taaaccgaat ggccgaggat gaaagcggct ggcaactgcc gctcgccgcg ctggtgcctc
     5821 ccgttaaaca cgcagggcaa tgcgcagagc gctcaccgcg cgtatgccct gagcactctc
     5881 agccacacat tgcggcggac gagagcaccg tcagcctgat ttgcgacgcc ttccgcgagg
     5941 tggttggcga gtctgtcacg cccgcagaaa acttctttga ggcgggcgca acgtcgctga
     6001 atctggtgca actgcacgtt ttgttacaac gtcacgaatt ttccaccctg acgttgcttg
     6061 acctcttcac ccacccttct cctgctgccc tggccgatta tctggccggc gtcgccacgg
     6121 tggagaaaac aaaacgacct cgccctgttc gccgtcgtca gcggcggata tagcgcgaag
     6181 caaactgatt ttccccggaa cgccatcgcg aacgcatggc gttccattga ctttgtgaat
     6241 cttaggaaac gggaccgatt atggataact tgcgcttctc ttctgcgccg acagcagatt
     6301 ccattgatgc atcgatcgct caacactacc cggactgcga acctgtcgcg gttatcggct
     6361 acgcctgcca ttttcctgaa tcgccggatg gcgaaacgtt ctggcaaaat ctgctggaag
     6421 gtcgtgaatg cagccgacgc tttacgcgcg aagagcttct ggccgtcggt ctggatgccg
     6481 ccatcattga cgatcctcat tatgtcaata tcggtacggt gttagacaac gccgactgct
     6541 tcgacgccac cctgtttggc tattcgcgac aggaagcgga gtcgatggac ccgcagcagc
     6601 gcctgttttt gcaggcggtc tggcatgcgc tggaacatgc cggttatgcc cccggcgccg
     6661 tcccccataa gaccggcgtt ttcgcctctt cccggatgag tacctacccc ggtcgcgaag
     6721 cattgaacgt gacagaagtc gcgcaggtaa aaggtctgca atctctgatg ggcaatgata
     6781 aagactatat tgccacccgc gccgcgtaca aactcaacct gcacggcccg gcgttatcgg
     6841 tacagaccgc ctgctccagc tcgctggttg ccgtgcatct ggcctgtgaa agcctgcgcg
     6901 caggcgaatc cgatatggcg gtggccggcg gcgtggcgct ctctttcccc cagcaggcgg
     6961 gctatcgcta ccagcccgga atgattttct ctcccgatgg ccactgtcgt ccttttgacg
     7021 cctcggctga gggcacctgg gccggtaatg gtctcggctg cgtggttctg cgtcgcctga
     7081 aagacgcgct actgtcaggc gatccgatta tctcggtgat cctctccagc gcggtcaata
     7141 acgacggcaa cagaaaggtc ggctataccg ccccttccgt cgccgggcag caggcggtca
     7201 tcgaagaggc gttaatgctg gcggccatcg acgacaggca ggtaggttac attgaaaccc
     7261 acggcaccgg cacaccgctg ggcgatgcga ttgaaataga agcgttacgc aacgtctatg
     7321 cgccgcgccc gcaggacagg cgctgtgcgc tcggttccgt gaaaagcaat atggggcatc
     7381 tggataccgc ggcaggcatt gccggactgc tgaaaaccgt tctggccgtc agtcgcggac
     7441 aaattccacc cttattgaat ttccacaccc ccaacccggc gctgaagctt gaggagagcc
     7501 cctttaccgt accagtgtcg gcgcaagcgt ggcaggatga aatgcgctat gcgggcgtat
     7561 cctcctttgg tattggcggc accaactgcc atatgatcgt cgcctcgctg cccgatgcgc
     7621 tcaacgcgcg cctccccaat acggatggcg gcagaaaaag tactgcgctg ctgctcagcg
     7681 ccgccagcga cagcgcgttg cggcggctgg cgacggatta tgccggggcg ctgaggaaga
     7741 atgcggatgc cagcgatctg gccttcacgg ccctgcacgc gcgccgtctc gatcttcctt
     7801 tccgcctggc ggtgccatta aaccgtgaaa ccgccgacgc gctcagcacc tgggctagtg
     7861 agaaatcggg ggcgcaggta tacagcggcc acggcgccag cggcaagcag gtgtggctgt
     7921 ttaccggcca gggctcgcac tggcgcacta tgggccaaac gatgtaccag cactcaacgg
     7981 cgtttgccga cacgctggat cgctgttttt ccgcctgtag cgaaatgctc acgccgtcac
     8041 tgcgcgaagc gatgtttaac cccgattcgg cgcagctgga caatatggcc tgggcgcagc
     8101 cggcgattgt cgcgtttgaa atcgcgatgg cggcgcactg gcgtgctgaa ggactgaagc
     8161 cagacttcgc cattgggcat tccgtcggtg aatttgccgc tgccgttgtc tgcggacact
     8221 atacgattga acaggtcatg ccactggttt gtcggcgcgg cgcgctaatg cagcagtgcg
     8281 caagcggcgc gatggtggcg gtatttgcag acgaagacac gctgatgccg ctggctcgcc
     8341 agtttgagct ggatctcgcc gccaacaacg gtacgcaaca tacggtattt tccgggccgg
     8401 aagcccgtct cgcggtattt tgcgccacgc tctcgcagca tgacattaac tatcgtcgcc
     8461 tgagcgtaac cggtgcggcg cactccgctt tactggagcc gatactcgat cggttccagg
     8521 acgcctgcgc gggactgcac gcggagccgg ggcaaatacc gattatttcc acgctcaccg
     8581 ccgacgtcat tgatgagtca acgctcaacc aggcggatta ctggcgccga cacatgcgcc
     8641 agccggtgcg ttttatccag agtattcagg tggcgcatca gctcggcgcc cgcgtttttc
     8701 tggagatggg gcccgatgcc cagttggttg cttgcgggca gcgcgaatac cgcgataacg
     8761 catactggat agccagcgcc cggcgtaaca aagaggcgag cgatgtcctc aatcaggccc
     8821 tgctccagct ttacgctgcc ggcgtcgccc taccgtgggc cgacctgctg gcgggcgatg
     8881 gacaacgtat cgctgcgcca tgttatccgt ttgatactga acgttactgg aaagagcgcg
     8941 tctccccggc ctgcgagcct gccgacgcag cgctgtctgc cgggctggag gtggcgagtc
     9001 gcgccgcgac agcgctcgat ctccctcgcc tggaagcgct taaacagtgc gccacgcgac
     9061 tgcacgccat ctacgtcgat caactggtac aacgctgtac cggcgatgcc attgagaacg
     9121 gcgtggacgc catgaccatc atgcgccgtg gacgtctgct gccccgctac cagcagctac
     9181 tccagcgcct gctgaataac tgcgtggtcg acggcgatta ccgctgcacc gacgggcgat
     9241 acgtccgcgc ccgccccatt gaacatcaac agcgggaatc actgctgacg gaacttgccg
     9301 gttattgtga aggttttcag gctattcccg acaccatcgc ccgtgccggc gatcggttat
     9361 atgaaatgat gagcggcgcg gaagaaccgg tggcgattat cttcccgcaa agcgcctccg
     9421 acggcgtgga agtgctgtat caggaattca gctttggccg ctatttcaac caaatcgccg
     9481 ccggggtatt acgcggcatt gtccagacgc gtcagccccg ccagccgttg cgtattcttg
     9541 aagttggcgg cggaaccggc ggcaccaccg cgtggctgct gccggaactc aacggcgttc
     9601 cggcactgga gtaccatttc accgatatct cggcgctgtt cacccgtcgc gcccagcaga
     9661 aattcgccga ctatgatttt gtgaagtata gcgagctgga tctcgaaaaa gaggcgcagt
     9721 ctcagggttt ccaggcacag tcttacgatc ttatcgtggc agcgaacgtg attcacgcca
     9781 cccgccatat tggccgcacg ctcgataatc tgcgccccct gctcaagccg ggcgggcgcc
     9841 tgctgatgcg cgaaatcacc cagccaatgc gtctgtttga cttcgttttc ggcccgctgg
     9901 ttcttccgct acaggatctc gacgcccgcg aaggtgagtt attcctcacc accgctcagt
     9961 ggcaacaaca gtgccgccac gccggattca gcaaagtggc gtggctaccg caggatggca
    10021 gcccgaccgc cgggatgagc gaacatatca ttctcgccac gctgcccggt caggcggtta
    10081 gcgccgtaac attcaccgcg ccatcagaac ccgtgttggg gcaggcgctg acggataacg
    10141 gtgattatct cgccgactgg tctgattgcg caggtcagcc cgaacggttt aacgcccgtt
    10201 ggcaggaggc ctggcgtctg ctttcacagc gtcatggcga cgctctgcct gtggaaccgc
    10261 cccccgtcgc cgccccggag tggctaggga aggttcgctt aagctggcaa aacgaagcct
    10321 tttcccgcgg tcagatgcgc gttgaagccc gtcatcctgc tggcgagtgg ctgccgctat
    10381 cgcccgccgc gcctcttcct gcgccgcaaa cgcattatca atggcgctgg acgcccctca
    10441 acgtcgccag cattgaccat ccgcttacct ttagcttcag cgccggtacg cttgcgcgca
    10501 gcgacgagct ggcgcaatac ggcatcattc acgatccgca cgcctcttca cgactgatga
    10561 ttgttgagga gagcgaggat acgctggcct tagcggagaa agtgatagca gcgctcaccg
    10621 ccagcgcagc cggattgatt gtggttactc gccgcgcgtg gcgagtcgag gaaaatgaag
    10681 cactctctgc atcccatcac gcgctatggg ccttgcttcg cgtcgcggcc aacgaacagc
    10741 cggaacggtt gcttgccgcc atcgatctcg ccgaaaacac cccgtgggaa acgctgcatc
    10801 aagggttgag tgcagtctca ctatcacagc gctggctcgc cgcacggggt gacacccttt
    10861 ggcttccttc actgtcgccc aatacgggat gcgccgctga attaccggca aacgtgttta
    10921 ccggcgatag ccgctggcat ctggtgaccg gagcgtttgg cggattaggc cgccttgccg
    10981 tgaactggct cagagaaaaa ggggcgcgac gcatcgccct gctggcgccg cgcgtggatg
    11041 agtcatggct acgcgacgtg gagggcgggc agacgcgcgt ctgccgttgt gatgtgggcg
    11101 atgccgggca actggccacg gttcttgacg atctggcggc caacggcggc attgccggag
    11161 cgattcatgc cgctggcgta ttggctgacg cgcccttgca ggagcttgat gaccaccagc
    11221 tggctgccgt tttcgcggta aaagcgcagg cggcaagcca gctgttgcaa accctgcgca
    11281 accacgacgg acgctatctt attctctact cttccgctgc cgccaccctc ggcgcgccgg
    11341 gtcagagcgc ccatgcgctg gcctgcggct acctggacgg gctggcccag cagttttcca
    11401 cccttgatgc gccgaaaacg ctctctgtcg cctggggcgc atggggagaa agcggtcggg
    11461 cggccacgcc ggaaatgctg gcgacgctcg ccagccgagg tatgggcgcg ttaagcgatg
    11521 ccgaaggctg ctggcacctg gaacaggcgg tgatgcgcgg cgccccgtgg cgactggcga
    11581 tgcgcgtttt taccgacaaa atgcccccgt tacaacaggc tctgtttaac atcagcgcca
    11641 cagaaaaagc cgcaacgccg gtcattcctc ctgctgatga caacgccttt aacggcagcc
    11701 tgagcgatga aacagcggtg atggcatggc tgaaaaagcg gattgcggtt cagctaaggc
    11761 tgagcgatcc ggcgtcactg catccaaacc aggatctgtt gcaactcggc atggactcgc
    11821 tgctcttcct tgaactcagt agcgatattc agcactacct gggcgtacgc atcaatgcgg
    11881 aacgggcgtg gcaggatctg tctcctcatg gactcacgca gcttatctgt tctaagccag
    11941 aggcgacgcc tgccgcttcg cagccggaag tgttgcggca cgacgccgac gagcgttatg
    12001 cgcccttccc tttgacgccc attcagcacg cctactggct ggggcgaacc cacctcattg
    12061 gctatggcgg cgtcgcctgt cacgtcctgt ttgagtggga taaacgccac gatgagttcg
    12121 atctcgccat actggagaaa gcatggaacc agctcatcgc acgccacgat atgttgcgta
    12181 tggtggttga tgccgacggg cagcagcgaa tcctggcgac aacgccggag tatcacatcc
    12241 cgcgtgacga tctgcgcgcg ctttccccgg aagaacagcg catcgcgctg gaaaaacggc
    12301 ggcatgaact gagctatcgc gttttgcctg ccgaccagtg gcctcttttt gagctggtgg
    12361 tcagcgaaat cgacgattgc cattaccgtc tgcatatgaa cctcgacctt ttgcagtttg
    12421 atgtgcagag ttttaaagtc atgatggacg acctggcgca ggtctggcgc ggtgaaacgc
    12481 tggcgccgct cgctattacc ttccgtgatt atgtgatggc tgaacaggcg cgccgacaga
    12541 catcggcatg gcacgatgcc tgggattact ggcaggaaaa actgccgcaa ctgcccttag
    12601 cgccagagct gccggtggtt gagacgcccc cggaaacgcc acacttcacc accttcaaat
    12661 cgacgatcgg caagacagaa tggcaggccg tgaaacagcg ctggcagcag caaggcgtca
    12721 caccgtctgc cgcgctgctc acgctgtttg ccgccaccct tgagcgctgg agccgtacca
    12781 caacatttac gctgaacctg acgttcttca atcgccagcc gatccatccg caaatcaacc
    12841 agttgattgg tgattttacc tccgtcacgc tggttgattt taacttctca gcgccggtga
    12901 cgttgcaaga gcagatgcaa cagacccaac agcgcctctg gcaaaacatg gcgcacagtg
    12961 aaatgaacgg tgttgaggtg atccgtgagc tgggccgcct gcgcggatca caacgtcaac
    13021 cgctgatgcc ggtagtgttt accagtatgc tggggatgac gctggaaggc atgactatcg
    13081 atcaggcgat gagccatctg ttcggcgaac cctgctatgt attcacgcaa acgccgcagg
    13141 tctggctgga tcatcaggtc atggagagcg acggcgagtt gatgtttagc tggtactgca
    13201 tggacaacgt gctggaaccc ggcgctgccg aggcgatgtt taatgactat tgcgccatcc
    13261 tgcaagccgt catcgccgcc cctgaaagcc tgaagactct cgccagcggc atcgccgggc
    13321 acattccccg ccgacgctgg ccgctgaacg cgcaggcgga ctacgacctg cgggatattg
    13381 agcaggcgac gctcgaatac cccggcatcc ggcaggccag agcggaaata accgaacagg
    13441 gcgcgttgac gctggatatc gtgatggccg acgatccgtc gccatcagcg gcgatgcctg
    13501 atgagcacga acttacccaa ctggcgctgc cgttgcctga gcaggcgcag cttgatgagc
    13561 tggaggcgac ctggcgctgg ctggaggcgc gtgcgctaca ggggatcgcg gctacgctaa
    13621 atcgtcacgg cctgtttacc acgccggaga tcgcccatcg ctttagcgca gtagtacagg
    13681 cgctgtccgc gcaagcgtct caccagcgtc tgctgcgcca gtggctacag tgtctgacgg
    13741 aaagagagtg gttaatccgc gaaggtgaaa gctggcgctg ccgcattccg ctcagcgaga
    13801 ttcctgagcc tcaggaagcg tgcccgcaaa gccaatggag ccaggcgctg gcgcagtatc
    13861 tggaaacctg catcgcccgg cacgacgccc tcttctccgg gcagtgttct ccgctggaat
    13921 tgctgttcaa cgagcagcat cgcgttaccg acgcgctgta tcgcgacaac cccgccagcg
    13981 cctgtctgaa tcgctatacc gcgcagattg ccgccttgtg cagcgcagaa cggattctgg
    14041 aggttggcgc cggaaccgca gccactaccg cgccggtgct gaaggccacg cggaacacgc
    14101 ggcagtcgta ccacttcacg gacgtctccg cgcagttcct caatgacgcc agagcccgtt
    14161 tccatgatga atcgcaggtg tcttatgcct tgttcgacat caaccagccg ctggatttca
    14221 ccgcccaccc ggaggcgggt tacgacctga tcgttgccgt caatgtgctc cacgacgcca
    14281 gccatgtcgt ccagacgttg cgcagattaa aactgttgct gaaagccggc ggacgtttgc
    14341 tgatcgttga agcgacggag cgaaacagcg tattccagct ggcgagcgtg ggctttattg
    14401 agggattaag cggataccgc gatttccgcc gccgggatga gaaaccgatg ctcacccgct
    14461 ccgcatggca ggaggttctc gttcaggccg ggtttgcaaa cgagctggcg tggcccgcgc
    14521 aggaatcgtc gccgctgcgc cagcatctgc tggtagcgcg ttcgcctggc gtaaatcgcc
    14581 cggataaaaa agccgtgagc cgctatttac agcagcgctt tggcaccggt ctgcccattt
    14641 tacagatccg gcaaagagaa gcgttattta cgccgctcca tgccccgtct gatgcgccga
    14701 ctgagccagc caaacccacg ccagttgccg gggggaattc ggcgctggaa aaacaggtgg
    14761 ctgaactctg gcaatcgctg ctgtctcgcc ccgtggcaag gcatcacgac tttttcgaac
    14821 tgggcggcga cagcctgatg gcgacaagga tggtcgcgca gctaaaccgg agagggattg
    14881 ccagggctaa ccttcaggat ctgttcaacc attcgacgct gagcgacttc tgcgcccatc
    14941 tacaggcggc tacgtcagga gaggacaacc cgatacccct ttgccagggc gacggtgagg
    15001 aaaccctgtt tgtcttccac gcttcagacg gcgatatcag cgcctggctg ccgctcgcta
    15061 acgcgttgaa caggcgcgtt ttcggcctgc aagcaaaatc gccgcagcgc tttgccacgc
    15121 tcgaccagat gatcgatgag tatgtcgggt gcatccgtcg tcagcagcct cacggccctt
    15181 atgtgctggc gggttggtcg tatggcgcgt ttcttgcggc gggcgccgca cagcgcctgt
    15241 acgccaaagg caagcaggtt cggatggtgt taatcgatcc cgtgtgccga caggatttct
    15301 gttgcgaaaa ccgggcggcc ctgctgcgcc tgttagccga aggacaaacg cctctggcac
    15361 tgcccgaaca tttcgaccag cagacgcccg acagccagct tgccgacttt atcagcctcg
    15421 ctaaaacggt cggtatggtg tcgcaaaacc tgacgctgca agcggcagaa acgtggctcg
    15481 acaacatcgc gcatctgctg cgtttactga ctgagcatac gccgggcgaa agcgttccgg
    15541 tcccctgtct catggtgtat gccgccggga gacccgcgcg ctggacgcca gcagaaaccg
    15601 agtggcaggg ctggataaac aacgccgacg acgctgtgat tgaagccagc cactggcaaa
    15661 tcatgatgga agccccccac gttcaggctt gtgcgcaaca cattacgcgc tggctttgcg
    15721 caacctcaac gcaaccggag aacacgttat gatgccgtcc gcctccccaa agcaacgcgt
    15781 actgattgtg ggcgccaaat ttggcgaaat gtacctgaat gcctttatgc agcccccgga
    15841 ggggctggaa ctggtcggcc tgctggcgca gggaagcgcc cgttcaagag agctggctca
    15901 tgcgtttggc attccgctgt atacctcgcc ggaacagata accaggatgc cggatatcgc
    15961 ctgtattgtc gtgcgttcga ccgtcgccgg aggaaccggt acgcagcttg ccagacactt
    16021 tctgacgcgc ggcgtacacg tcattcagga gcatcccctc cacccggatg acatcagttc
    16081 cttacaaacg ctggcgcagg aacagggctg ctgctattgg gtaaacacgt tttatccgca
    16141 tacgcgcgcg ggacgcacat ggcttcgcga tgcgcagcag ctgcgtcgct gcctggctaa
    16201 gacgccgccg gtagtccacg ccaccaccag ccgacagttg ctctactcca ctctggattt
    16261 gttgttgctg gctctgggcg ttgatgccgc cgccgtggag tgcgacgtgg ttggcagctt
    16321 tagcgatttt cactgtctga gactcttctg gccggaggga gaagcctgcc tgctgcttca
    16381 gcgctatctc gatcctgacg atccagatat gcatagcctt atcatgcacc gcctgctgct
    16441 gggctggccg gaaggtcatc tttcgctgga ggcgagctac ggccccgtca tctggtcatc
    16501 cagcctgttt gtcgcagacc atcaggagaa cgcccacagc ctctaccgca gaccggagat
    16561 cctgcgcgat ctgccgggtc tgatgcgcag cgcggcgccc ctgagctggc gcgactgctg
    16621 cgaaaccgtc gggccggagg gcgtcagctg gttgctacac cagttgcgca gcaatctggc
    16681 gggcgaacat ccaccagcgg cctgccaaag cgtacatcag atcgcgttat cacgtttatg
    16741 gcagcagatt ttacgtaaga ccggcaatgc ggagatcaga cgcctgacgc cgccgcacca
    16801 cgaccggtta gccggtttct acaacgatga tgataaggag gcgctgtgac gcaatctgca
    16861 atgtgcatcc cgctgtggcc cgcccggaac ggcaatactg cgcatctggt catgtgccct
    16921 ttcgctggcg gcagcagtag cgcgtttcgc cactggcaag ctgagcaact ggctgattgc
    16981 gcgctttctc tggtgacctg gccggggcgc gatcgccttc gccatctgga accgctcaga
    17041 agcattacac aactggcggc actgctggcg aacgagctgg aagcatccgt atcgcctgac
    17101 acgccgcttt tactcgccgg gcacagcatg ggggcgcagg tggcgtttga aacctgccga
    17161 cttctggagc aacgggggct tgcgccacaa ggactgatta tttccgggtg ccatgccccg
    17221 catctgcatt ctgaacgcca gctcagccat cgcgatgatg ccgactttat cgctgagctg
    17281 atagacattg gcggatgttc tcctgaactg cgggaaaacc aggaattaat gtcgctgttt
    17341 cttcctcttc tgcgcgctga tttttacgcc accgagagct atcactacga ctcgcccgac
    17401 gtctgtccgc cgctgcgcac gcctgcgctg ttattgtgcg gcagccacga tcgcgaagcc
    17461 tcctggcagc aggtcgatgc ctggcgtcag tggctgagcc acgttacagg cccggtggtg
    17521 attgacggcg atcacttcta tcccattcaa caagcccggt ccttttttac gcagattgtc
    17581 cgccattttc cccacgcatt ttctgcaatg accgcgtggc aaaaacagcc cagtacttca
    17641 gaaaggtgac gcatgaattc ttcctttgaa tctctgattg aacagtatcc cttacccatt
    17701 gccgaacagt tgcgccactg ggcggcccgt tatgcctcgc gaattgccgt cgttgatgca
    17761 aaggggtcgt taacctacag cgcgcttgat gcacaagttg acgaacttgc cgcaggtctg
    17821 tcatcactgg gtttgcgttc gggggagcat gtaattgtgc agcttcccaa cgacaacgcg
    17881 tttgttaccc tgctgttcgc cttgttaaga ctgggcgtta tccccgtgct ggcgatgccc
    17941 tcgcaacggg cgctggatat cgacgcgctg attgagctgg cgcaacccgt cgcttacgtt
    18001 attcacgggg aaaaccacgc agagctggcc cgacagatgg cgcacaaaca cgcctgcttg
    18061 cgtcatgttc tggtcgctgg agagaccgtg agcgacgatt ttacgccgct cttctccctt
    18121 cacggtgagc gacaggcatg gccgcagcct gatgtttccg ccaccgcgtt gttgttgctc
    18181 tcaggcggca caaccggcac gcccaaactc atcccgcgcc gacatgccga ctatagctat
    18241 aacttcagcg cttctgctga actgtgcggc atcagccaac agagcgtgta tctcgccgtc
    18301 ctcccggtgg cgcataactt tccgctggcc tgccccggta ttctgggaac gcttgcctgc
    18361 ggcggaaaag tggtgctgac cgacagcgcc agctgtgatg aggtgatgcc tttaatcgcg
    18421 caggaaagag tgactcacgt cgccctggtt ccggcgctgg cgcaattatg ggtgcaggcc
    18481 agggagtggg aagacagcga cctttcgtcg ctgcgcgtca ttcaggcagg cggcgcccgg
    18541 ctcgacccga cgcttgctga gcaggttatc gccacctttg actgtaccct gcaacaggtt
    18601 ttcggtatgg cggaaggcct gctctgtttt acccggctgg acgatccgca tgccaccatt
    18661 ctccacagcc aggggcgccc gttgtcccct ctggatgaaa tccgcatcgt tgatcaagac
    18721 gagaacgacg tcgcgccggg cgaaaccggg caattgttaa cgcgcggccc ttataccatt
    18781 tcgggctatt accgcgcccc tgcccacaac acgcaggcct ttaccgcgca agggttttac
    18841 cgcacaggcg acaatgtcag gctggatgag gtggggaacc tgcacgttga gggacgcata
    18901 aaagagcaga tcaaccgcgc cggagaaaaa atagccgcgg ctgaagtgga atcggcactg
    18961 ctgcgtttag cggaagtgca agattgcgcg gtggtcgccg cgccggacac gctgcttggc
    19021 gagcggattt gcgcgtttat catcgcgcag caggtgccaa ctgactatca gcagttgcgt
    19081 caacaactga cccgtatggg gctcagcgcg tggaaaattc ctgaccaaat cgagtttctg
    19141 gaccactggc cgctcaccgc cgtcggcaag atagacaaaa aacgcctgac ggctctcgcc
    19201 gtcgaccgtt atcgccattc tgcccaataa gcgcaaaccg acccgaaaca ggttgaaata
    19261 aacccgtttc gggtagcacc actattagaa atagttatca ttttcaattc accattgtcg
    19321 gtatttttgg cgtttcgccg tcttacaggg actcacaaca atgaaaatga cacggcttta
    19381 tcctctggcc ttggggggat tattgctccc cgccattgct aatgcccaga cttcacagca
    19441 agacgaaagc acgctggtgg ttaccgccag taaacaatct tcccgctcgg catcagccaa
    19501 caacgtctcg tctactgttg tcagcgcgcc ggaattaagc gacgccggcg tcaccgccag
    19561 cgacaaactc cccagagtct tgcccgggct caatattgaa aatagcggca acatgctttt
    19621 ttcgacgatc tcgctacgcg gcgtctcttc agcgcaggac ttctataacc ccgccgtcac
    19681 cctgtatgtc gatggcgtcc ctcagctttc caccaacacc atccaggcgc ttaccgatgt
    19741 gcaaagcgtg gagttgctgc gaggcccaca gggaacgtta tatggcaaaa gcgctcaggg
    19801 cgggatcatc aacatcgtca cccagcagcc ggacagcacg ccgcgcggct atattgaagg
    19861 cggcgtcagt agccgcgaca gttatcgaag taagttcaac ctgagcggcc ccattcagga
    19921 tggcctgctg tacggcagcg tcaccctgtt acgccaggtt gatgacggcg acatgattaa
    19981 ccccgcgacg ggaagcgatg acttaggcgg cacccgcgcc agcataggga atgtgaaact
    20041 gcgtctggcg ccggacgatc agccctggga aatgggcttt gccgcctcac gcgaatgtac
    20101 ccgcgccacc caggatgcct atgtgggatg gaatgatatt aagggccgta agctgtcgat
    20161 cagcgatggt tcaccagacc cgtacatgcg gcgctgcact gacagccaga ccctgagtgg
    20221 gaaatacacc accgatgact gggttttcaa cctgatcagc gcctggcagc agcagcatta
    20281 ttcgcgcgcc ttcccttccg gttcgttaat cgtcaatatg cctcagcgct ggaatcagga
    20341 tgtgcaggag ctgcgcgccg caaccctggg cgatgcgcgt accgttgata tggtgtttgg
    20401 gctgtaccgg cagaacaccc gcgagaagtt aaattcagcc tacgacatgc cgacaatgcc
    20461 ttatttaagc agtaccggct ataccaccgc tgaaacgctg gccgcataca gtgacctgac
    20521 ctggcattta accgatcgtt ttgatatcgg cggcggcgtg cgcttctcgc atgataaatc
    20581 cagtacacaa tatcacggca gcatgctcgg caacccgttt ggcgaccagg gtaagagcaa
    20641 tgacgatcag gtgctcgggc agctatccgc aggctatatg ctgaccgatg actggagagt
    20701 gtatacccgt gtagcccagg gatataaacc ttccgggtac aacatcgtgc ctactgcggg
    20761 tcttgatgcc aaaccgttcg tcgccgagaa atccatcaac tatgaacttg gcacccgcta
    20821 cgaaaccgct gacgtcacgc tgcaagccgc gacgttttat acccacacca aagacatgca
    20881 gctttactct ggcccggtcg ggatgcagac attaagcaat gcgggtaaag ccgacgccac
    20941 cggcgttgag cttgaagcga agtggcggtt tgcgccaggc tggtcatggg atatcaatgg
    21001 caacgtgatc cgttccgaat tcaccaatga cagtgagttg tatcacggta accgggtgcc
    21061 gttcgtacca cgttatggcg cgggaagcag cgtgaacggc gtgattgata cgcgctatgg
    21121 cgcactgatg ccccgactgg cggttaatct ggtcgggccg cattatttcg atggcgacaa
    21181 ccagttgcgg caaggcacct atgccaccct ggacagcagc ctgggctggc aggcgactga
    21241 acggatgaac atttccgtct atgtcgataa cctgttcgac cgtcgttacc gtacctatgg
    21301 ctacatgaac ggcagcagcg ccgtcgcgca ggtcaatatg ggtcgcaccg tcggtatcaa
    21361 tacgcgaatt gatttcttct gattattgta aaagggatac cgaaaaggta t
//
LOCUS       DAOTRK010000079        21257 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.079, whole genome shotgun sequence.
ACCESSION   DAOTRK010000079 DAOTRK010000000
VERSION     DAOTRK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.079"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            231..1274
                     /gene="nadA"
                     /locus_tag="QIE41_002800"
     CDS             231..1274
                     /gene="nadA"
                     /locus_tag="QIE41_002800"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDS3531673.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1312..2031
                     /gene="pnuC"
                     /locus_tag="QIE41_002801"
     CDS             1312..2031
                     /gene="pnuC"
                     /locus_tag="QIE41_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDS3531674.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASMEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2028..2969)
                     /gene="zitB"
                     /locus_tag="QIE41_002802"
     CDS             complement(2028..2969)
                     /gene="zitB"
                     /locus_tag="QIE41_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDS3531675.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREISEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3083..3463)
                     /locus_tag="QIE41_002803"
     CDS             complement(3083..3463)
                     /locus_tag="QIE41_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDS3531676.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3779..4831
                     /gene="aroG"
                     /locus_tag="QIE41_002804"
     CDS             3779..4831
                     /gene="aroG"
                     /locus_tag="QIE41_002804"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDS3531677.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGTPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(4997..5749)
                     /gene="gpmA"
                     /locus_tag="QIE41_002805"
     CDS             complement(4997..5749)
                     /gene="gpmA"
                     /locus_tag="QIE41_002805"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDS3531678.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5952..6992)
                     /gene="galM"
                     /locus_tag="QIE41_002806"
     CDS             complement(5952..6992)
                     /gene="galM"
                     /locus_tag="QIE41_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDS3531679.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGSTLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(6986..8134)
                     /gene="galK"
                     /locus_tag="QIE41_002807"
     CDS             complement(6986..8134)
                     /gene="galK"
                     /locus_tag="QIE41_002807"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDS3531680.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8138..9184)
                     /gene="galT"
                     /locus_tag="QIE41_002808"
     CDS             complement(8138..9184)
                     /gene="galT"
                     /locus_tag="QIE41_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDS3531681.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESKDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDGQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9194..10210)
                     /gene="galE"
                     /locus_tag="QIE41_002809"
     CDS             complement(9194..10210)
                     /gene="galE"
                     /locus_tag="QIE41_002809"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDS3531682.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10473..11945)
                     /gene="modF"
                     /locus_tag="QIE41_002810"
     CDS             complement(10473..11945)
                     /gene="modF"
                     /locus_tag="QIE41_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDS3531683.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETSA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            complement(12013..12801)
                     /gene="modE"
                     /locus_tag="QIE41_002811"
     CDS             complement(12013..12801)
                     /gene="modE"
                     /locus_tag="QIE41_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDS3531684.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12930..13079
                     /gene="acrZ"
                     /locus_tag="QIE41_002812"
     CDS             12930..13079
                     /gene="acrZ"
                     /locus_tag="QIE41_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDS3531685.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13246..14019
                     /gene="modA"
                     /locus_tag="QIE41_002813"
     CDS             13246..14019
                     /gene="modA"
                     /locus_tag="QIE41_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS3531686.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVIAFYDYLKGPQAAEIFKRYGFTTK"
     gene            14019..14708
                     /gene="modB"
                     /locus_tag="QIE41_002814"
     CDS             14019..14708
                     /gene="modB"
                     /locus_tag="QIE41_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDS3531687.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14711..15769
                     /gene="modC"
                     /locus_tag="QIE41_002815"
     CDS             14711..15769
                     /gene="modC"
                     /locus_tag="QIE41_002815"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDS3531688.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15770..16588)
                     /gene="ybhA"
                     /locus_tag="QIE41_002816"
     CDS             complement(15770..16588)
                     /gene="ybhA"
                     /locus_tag="QIE41_002816"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDS3531689.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLAKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIARFIYSHLI"
     gene            16743..17738
                     /gene="pgl"
                     /locus_tag="QIE41_002817"
     CDS             16743..17738
                     /gene="pgl"
                     /locus_tag="QIE41_002817"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDS3531690.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17779..18732)
                     /locus_tag="QIE41_002818"
     CDS             complement(17779..18732)
                     /locus_tag="QIE41_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3531691.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKVGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLVKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            18916..19968
                     /locus_tag="QIE41_002819"
     CDS             18916..19968
                     /locus_tag="QIE41_002819"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDS3531692.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFTEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPTELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20044..21156
                     /locus_tag="QIE41_002820"
                     /pseudo
     CDS             20044..21156
                     /locus_tag="QIE41_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT         5088 a         5228 c         5534 g         5407 t
ORIGIN      
        1 tcaatttcaa ttcttaaccc tgatgacata ctccgcgctt gctgcgtaaa caactctacg
       61 tcggaggaat aagacgcaaa gtgtggcatt cgtcatctca tacgtaattc gagtgacttt
      121 tccccaccat tcgactatct tgtttagcat ataaaacaaa tttcaccgat aacagcgaat
      181 attacgctca tgtcggtttt aacgttaagc ctgtaaaacg agatggtaag atgagcgtga
      241 tgtttgatcc agacacggcg atttatcctt tccccccgaa gccgacgccg ttaagcattg
      301 atgaaaaagc gtattaccgc gagaagataa aacgtctgct aaaagaacgt aatgcggtga
      361 tggttgccca ttactatacc gatcccgaaa ttcaacaact ggcagaagaa accggtggct
      421 gtatttctga ttctctggaa atggcgcgct tcggtgcaaa gcatcccgct tctacgttgt
      481 tagtcgctgg ggtgagattt atgggagaaa ccgccaaaat tctcagtccg gaaaaaacaa
      541 ttctgatgcc gacacttcag gctgaatgtt cactggatct cggctgccct gttgaagaat
      601 ttaacgcatt ttgcgatgcc catcccgatc atactgtcgt tgtctacgcc aacacttctg
      661 ctgcggtaaa agcgcgggca gattgggtgg taacttcaag cattgccgtc gaacttattg
      721 atcatctgga tagtttgggt gaaaaaatca tctgggctcc cgacaaacat ctggggcgtt
      781 acgtgcaaaa acagacgggc gcagacattt tatgctggca gggtgcctgc attgtgcatg
      841 atgaatttaa gactcaggcg ttaacccgct tgcaagaaga atacccggat gctgccatac
      901 tggtgcatcc agaatcacca caagctattg tcgatatggc ggatgcggtc ggttccacca
      961 gtcaactgat cgctgctgcg aaaacattgc cacatcagag gcttattgtg gcaaccgatc
     1021 ggggtatttt ctacaaaatg cagcaggcgg tgccagataa agagttactg gaagcaccaa
     1081 ccgcaggtga gggtgcaacc tgccgcagct gcgcgcattg tccgtggatg gccatgaatg
     1141 gccttcaggc catcgcagag gcattagaac aggaaggaag caatcacgag gttcatgttg
     1201 atgaaaggct gcgagagagg gcgctggtgc cgctcaatcg tatgctggat tttgcggcta
     1261 cactacgtgg ataacgaata ataaggcgta acgttacgct ttgggggaaa gatggatttt
     1321 tttagtgtgc agaatatcct ggtacatata ccaataggag caggcggtta tgatctctca
     1381 tggatcgaag cggtaggcac gatcgctggg ttgctgtgta ttggccttgc cagtatggag
     1441 aagatcagca actacttctt tggcctgatc aacgtcacct tgtttggcat tattttcttt
     1501 cagattcagc tgtatgccag cctgctatta caggtgtttt tctttgccgc gaatatttac
     1561 ggttggtatg cgtggtcgcg acaaaccagt cagaacgagg cggagttgaa aattcgctgg
     1621 ttgccattgc cgaaggcgct cagctggttg gcggtttgcg ttgtttcgat tggtctgatg
     1681 acggtattta tcaatccggt gtttgcattt ttgacccgcg tggcagtcat gatcatgcaa
     1741 gcattaggat tacaggttgc gatgcctgaa ctacagccgg acgctttccc gttctgggat
     1801 tcatgcatga tggtgttatc catcgtggca atgattctga tgacgcgtaa gtatgtggaa
     1861 aactggctgt tgtgggtgat tattaacgtg attagcgtcg ttatttttgc acttcagggc
     1921 gtttacgcca tgtctctgga gtacatcatc ctgaccttta ttgcgcttaa cggcagccgg
     1981 atgtggatca acagcgcacg cgaaagaggt tcacgcgcgc tgtcccatta atggtgatga
     2041 tgtgaatggc cagacacccc ttcattcagg tggcagtctg gcccatgaca aggttgatat
     2101 tccatctgaa tagtggcgtg ctcaatctga tagtgatcca tcaggtagtg ttggatctga
     2161 tctaacaagg catcgtgatc gtgtggcggg atcacctgca catgcagcgt catcaccggc
     2221 ttctcgccta ccatccatac atgtacatgg tgcacattgc ggacttccga gatttcccgg
     2281 cacatacgac gcttcagttc ggcgatatcc agcgataccg gtgcaccttc aagtaattca
     2341 ttcacgctat ctttcaacag tcgccacgcg ctacgcagaa ccagaagcga caccagtatt
     2401 gagagaatgg ggtcagcagg tgtccagccg gtccagataa taatcaacgc ggcaataata
     2461 gccccaaccg aacccagcag gtccccgagc acatgcagtg ccgctgctcg cacgttgagg
     2521 tttttctctt cgctgccgtg atgaagtaac cagaaagaaa gtatatttgc cagcagccca
     2581 gccacggcaa ttgccatcat catgccgccc tcgaccggac gcggagtgcg gaagcgttct
     2641 atcgcctccc agacaatcaa aatagtaatc accaccaggg cgatggcatt cacaaacgcc
     2701 gcgagcgtgg tcaatcttag ccaaccgaaa gtgtggcgaa tggtgggagg acgacgggaa
     2761 aattgcactg cgagtagggc gaaaagcagg gcggcagtat cggtcaacat atgacccgca
     2821 tcggccagca atgccagaga accggaaaga aaaccaccaa tgacttctac gagcataaac
     2881 ccggcagtca cgccgaaagc atacaacaag cgacgagcat tattatcttc tggcaggtgt
     2941 gaggaagtgt gtgagtgtga gtgcgccatg acgagtcatc ccttaattat tctcacttaa
     3001 ctttatgaca tcatactgct tttagaaggg aaaaattaaa agggagagac tccgctctcc
     3061 cattattggc tatttcgcag ggttactgcg tggtaccgtc ggttttggta tcgacatcat
     3121 tgttgatgcc atcaccagtt tgtacctttt tattgatatc cggacagcga ccatctttgc
     3181 acatggtgtt cttgtgctct tcatctttgg tcatgccgtc attgttcatt gaagaaccat
     3241 ccggatgcag cattgtgccg ccagaaccgg tatttacccc gttattgtcg acgttatttg
     3301 gcgcgacatt ttcacgggca tcaggggcta cctggcccgc atcagctgcg gcgtttgcct
     3361 ggccgttatt gctttgcgct ccgctatcgg cagccagtgc ggcaccgctg gcaaggctta
     3421 gagtggcagt caaaaatagt gtggccagtt ttgtcatttt cataggatgc tcctgttatg
     3481 gtcgttatgt cggataacct cttccaacag tgcatttgca ggtgaatata aggcaatggt
     3541 ttaagatttc agccaagcaa agatacccag cagagaatct tgaaataatt aacaaacaaa
     3601 ggagttacag ttagaaattg taggagagat ctcgtttttc gcgacaatct ggcgtttttc
     3661 ttgctaattc caggattaat ccgttcatag tgtaaaaccc cgtttacaca ttctgacgga
     3721 agatatagat tggaagtatt gcattcacta agataagtat ggcaacactg gaacagacat
     3781 gaattatcag aacgacgatt tacgcatcaa agaaatcaaa gagttacttc ctcctgtcgc
     3841 attgctggaa aaattccccg ctactgaaaa tgccgcgaat acggtcgccc atgcccgaaa
     3901 agcgatccat aaaatcctga aaggtaatga tgatcgcctg ttggtggtga ttggtccgtg
     3961 ctcaattcat gatcctgtcg cggctaaaga gtatgccgct cgcttgctga cgctgcgtga
     4021 agagctgcaa gatgagctgg aaatcgtgat gcgcgtctat tttgaaaagc cgcgtactac
     4081 ggtgggctgg aaagggctga ttaacgatcc gcatatggat aacagcttcc agatcaacga
     4141 cggtctgcgt attgcccgca aattgctgct cgatattaac gacagcggtc tgccagcggc
     4201 gggtgaattc ctggatatga tcaccccaca atatctcgct gacctgatga gctggggcgc
     4261 aattggcgca cgtactacgg aatcgcaggt gcaccgcgaa ctggcatcag ggctttcttg
     4321 cccggtaggc ttcaaaaatg gcaccgacgg tacgattaaa gtggctatcg atgccattaa
     4381 tgccgccggt acgccgcact gcttcctgtc cgtaacgaaa tgggggcatt cggcgattgt
     4441 gaataccagc ggtaacggcg attgccatat cattctgcgc ggtggtaaag agcctaacta
     4501 cagcgcgaag cacgttgctg aagtgaaaga agggctgaac aaagcaggcc tgccagcaca
     4561 ggtgatgatc gatttcagcc atgctaactc gtcaaaacaa ttcaaaaagc agatggatgt
     4621 ttgtgctgac gtttgccagc agattgccgg tggcgaaaag gccattattg gcgtgatggt
     4681 ggaaagccat ctggtggaag gcaatcagag cctcgagagt ggggaaccgc tggcctatgg
     4741 caagagcatc actgatgcct gcatcggctg ggaagatacc gatgctctgt tacgtcaact
     4801 ggcgaatgca gtaaaagcgc gtcgcgggta aggtttaatt gtcggatgcg ccgtcagagt
     4861 ggcatatccg atgaatcacc gtagccctga taagacgcgc agcgtagcat caggcaatgt
     4921 gctccattgt tagcaacgac aaacaaaaaa gccgactcac ttgcagtcgg ctttctcatt
     4981 tttaaacgaa tgacgtttac ttcgctttac cctggttcgc aacggctgct gctttcgccg
     5041 cgatctcgtc agcattaccc agataatagc gtttcagcgg tttgaagttc tcgtcaaact
     5101 cataaaccag cggcacgccg gtcgggatat tcagctcaag aatctcttct tcgctcatgt
     5161 tatcaagata tttcaccagc gcacgtaacg agttaccgtg tgcagcgatg attacgcgct
     5221 caccgctctt catacgcggt agaatagttt cattccagta agggatcacg cggtcaatgg
     5281 tcagcgccag gctttccgtc agtggcagtt ctttctcgct cagcttcgcg taacgtggat
     5341 cgtgacccgg ataacgctca tcatctttag tcagttccgg cggagtcact gcaaaaccac
     5401 gacgccactg tttcacctgc tcgtcgccat acttttcagc agtttccgct ttgttcagac
     5461 cctgcaacgc accgtagtga cgttcgttca gtttccagga tttctcaacg ggcagccatg
     5521 cctgatccag ttcgtccagc acattccaca gggtatgaat agcgcgtttc agcacagagg
     5581 tgtaagcaaa gtcaaagctg taaccttcct ctttcagcag cttacctgct gcttttgctt
     5641 cgcttacgcc tttctcagac agatccacgt cgtaccaacc ggtgaaacgg ttttctttgt
     5701 tccactgact ttcgccatga cgaaccagaa ccagcttagt tacagccata tacttactcc
     5761 tcaaatcatc ttttaatgat aataattctc attatattgc cgtgacgaag caacagcaat
     5821 gcttacgcat aaccatagcg aaaatagtgt cgcagtgtaa ggttgttgtg aatattgagt
     5881 tgcaaatatg tcggtgtttg ctggtgattt gaacaatatg aggataaagc cctcatgacg
     5941 agggcttaac attactgagc aataaactga tattccgtca ggctggaata ctcttcgcca
     6001 ggacgcagga agcagtctgg ttgcggccat tcagggtggt tcgggctgtc cggcagaaat
     6061 tcgctttcca gagccagccc ttgccagtcg gcgtaaggtt cggttccccg cgacggtgtg
     6121 ccgccgagga agttgccgga gtagaattgc agagccggag cggtggtgta gaccttcagc
     6181 tgcaattttt catctgctga ccagacatgc gccgccactt tcttgccatc gcctttggcc
     6241 tgtaacaaga acgcgtgatc gtaacctttc actttgcgct gatcgtcgtc ggcaagaaac
     6301 tcactggcga tgattttggc gctgcggaaa tcaaaagacg ttccggcgac agatttcagg
     6361 ccgtcgtgcg gaatgccgcc ttcatcaacc ggcagatatt cgtccgccag aatctgcaac
     6421 ttgtgattgc gcacgtcaga ctgctcgccg tcaagattga aatagacgtg attagtcata
     6481 ttcaccgggc aaggtttatc aactgtggcg cgataagtaa tggagatacg gttatcgtcg
     6541 gtcagacgat attgcaccgt cgcgccgaga ttacccggga agccctgatc gccatcatct
     6601 gaactcaggg caaacagcac ctgacgatca ttctggttca caatctgcca gcgacgtttg
     6661 tcgaaccctt ccgggccgcc gtgcagctgg ttaacgccct gacttggtga aagcgtcacg
     6721 gtttcaccgt caaaggtata acggctattg gcgatgcggt tggcataacg accaatagag
     6781 gcccccagaa atgcggcctg atcctgatag cattccgggc tggcgcagcc aagcagcgcc
     6841 tctcggacgc tgccatcgga aagcggaata cgggcggaaa gtaaagtcga accccagtcc
     6901 atcagcgtga ctaccatccc tgcgttgtta cgcaaagtta acagtcggta cggctgacca
     6961 tcgggtgcca gtgcgggagt ttcgttcagc actgtcctgc tccttgtgat ggtttacaaa
     7021 cgtaaaaagt ctctttaata cctgtttttg cttcatattg ttcagcgaca gcttgctgta
     7081 cggcaggcac cagctcttcc gggatcagcg cgacgataca gccgccaaat ccgccgccgg
     7141 tcatgcgtac gccacctttg tcgccaatca cagctttgac gatttctacc agagtgtcaa
     7201 tttgcggaac ggtgatttcg aaatcatcgc gcatagaggc atgagactcc gccatcaact
     7261 cgcccatacg tttcaggtcg ccttgctcca gcgcgctggc agcttcaacg gtgcgggcgt
     7321 tttcagtcag gatatgacgc acgcgtttcg ccacgattgg gtccagttca tgcgcaacag
     7381 cgttgaactc ttcaatggtg acatcgcgca gggctggctg ctggaagaaa cgcgcaccgg
     7441 tttcgcactg ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt ttgaagttac
     7501 tgttgataat gacgacagcc acacctttcg gcatggagac tgctttggta cccagtgaac
     7561 ggcaatcgat cagcaaggca tgatctttct tgccgagtgc ggaaattagc tgatccatga
     7621 tcccgcagtt acagccaaca aactggtttt ctgcttcctg gccgttaagc gcgatttgtg
     7681 cgccgtccag cggcagatga taaagctgct gcaatacggt tccgactgcg acttccagtg
     7741 aggcggaaga acttaacccg gcaccctgcg gcacgttgcc gctaatcacc atgtccacgc
     7801 cgccgaagct gttgttacgc agttgcagat gtttcaccac gccacgaacg tagttagccc
     7861 attgatagtt ttcatgtgcg acaatgggcg catcaaggga aaactcgtcg agctgatttt
     7921 cataatcggc tgccattacg cgaactttac ggtcatcgcg tggcgcacaa ctgatcacgg
     7981 tttgataatc aatcgcgcag ggcagaacga aaccgtcgtt gtagtcggtg tgttcaccaa
     8041 tcaaattcac gcggccaggc gcctgaatgg tgtgagtggc agggtagcca aatgcgttgg
     8101 caaacagaga ttgtgttttt tctttcagac tcatttctta cactccggat tcgcgaaaat
     8161 ggatatcgct gactgcgcgc aaacgctctg ctgcctgttc tgcggtcagg tctcgctggg
     8221 tctctgccag catttcataa ccaaccataa atttacgtac ggtggcggag cgcaacagag
     8281 gcggataaaa gtgcgcgtgc agctgccagt gttgattctc ttcgccatta aacggtgcgc
     8341 cgtgccagcc catagagtag gggaaggagc actggaagag gttgtcataa cgactggtca
     8401 gctttttcaa cgccagcgcc aaatcgctgc gctggccgtc ggtcaaatcg gtgatccgca
     8461 aaacgtgggc tttgggcagt agtagcgttt cgaacggcca ggcagcccag taaggcacta
     8521 cggctaacca gtgttcggtt tcgacaacgg tacggctacc gtctgccagc tcgcgctgaa
     8581 cataatccac cagcattggt gatttctgtt cggcaaaata ttctttttgc aggcggtctt
     8641 cacgctcagc ttcgttaggc aggaagctat ttgcccaaat ctgtccgtgc ggatgcgggt
     8701 tagagcagcc catcgccgca cctttgtttt caaagacctg cacccatggg tatgttttcc
     8761 ccagttctgc ggtttgctcc tgccaggttt tgacgatttc cgtcaatgcc gcaacgctca
     8821 gttctggcaa cgttttactg tgatccggtg aaaagcagat cacccggctg gtaccgcgcg
     8881 cgctctggca acgcatcagc ggatctttgc tttctggcgc atctggcgtg tcagacatca
     8941 aggccgcaaa gtcattagtg aaaacgtaag tcccggtgta atcggggttt ttatcgcctg
     9001 tcacccgcac attacctgcg cagaggaagc aatctggatc atgcgcaggt aacacctgtt
     9061 tggctggcgt ttcctgcgcc ccctgccagg ggcgcttagc gcggtgcggt gaaaccagaa
     9121 tccattgccc ggtgagcggg ttgtagcggc gatgtggatg atcaacggga ttaaattgcg
     9181 tcatggtcgt tccttaatcg ggatatccct gtggatggcg tgactgccag tgccaggtgt
     9241 cctgcgccat ttcatcgagt gtgcgcgtca cgcgccagtt cagttcacgg tcggctttgc
     9301 tggcgtccgc ccagtaggcc ggaaggtcgc cctcgcgacg cggtgcaaaa tgatagttaa
     9361 ccggtttgcc gcaggctttg ctgaaggcat taaccacgtc cagcacgctg ctgcctacgc
     9421 cagcgccgag gttgtagatg tgtacgcctg gcttgttcgc cagtttctcc atcgccacga
     9481 cgtgaccgtc cgccagatcc attacgtgga tgtaatcgcg cacgccagta ccgtcttcgg
     9541 tcggataatc gttaccaaaa atcgccagcg agtcgcgacg gcctacagca acctgggcga
     9601 tgtatggcat cagattattc ggaatgcctt gcggatcttc gcccatatcg cccgacggat
     9661 gcgcgccaac cgggttgaag tagcgcagca gggcaatgct ccagtccggc tgggcttttt
     9721 gcagatcggt gaggatctgt tccaccatta gcttgctttt gccgtaaggg ctttgcggtg
     9781 tgccggtcgg gaagctttca acgtatggaa ttttgggctg atcgccatag acggtggcgg
     9841 aggagctaaa aataaagttt ttgacgttcg cggcgcgcat ggcgctaatc aggcgcaggg
     9901 tgccgttgac attgttgtcg taatattcca gcggtttttg taccgattcg ccaacggctt
     9961 tcagcccggc gaagtggatc acggtgtcga tagcgtgatc gtgcaggatc tcggtcatca
    10021 acgcttcgtt acgaatatcg ccttcaacaa acgttggatg tttgccgcct aaacgctcga
    10081 taacgggcag tacgctgcgc ttactgttac agaggttatc aagaatgatg acatcatgac
    10141 cgttttgcag taattgcaca caggtatgac ttccaatgta accgctacca ccggtaacca
    10201 gaactctcat aattcgctcc attaggctta tggtatgaaa taaccatagc ataacaaaga
    10261 tgcgaaaagt gtgacatgga ataaattagt ggaatcgttt acacaagaat ttagccgttt
    10321 tttatgcgcg attaagtgat tataaaacag agggtttatg aatgattgcg ctttttatct
    10381 gaaaaaaaag acgcggtttc atgcctggat gcgtcgaacc gttggccgga gagggcgctt
    10441 aggccgcctc cggcacggtc agcactaccg actcaattta tttttgtcag cacatagcga
    10501 tagagtccgc cgtctggcac gaactcaaga cgatgggtaa tacaggcagg cgcatcttca
    10561 gcgtggtgcg aaacaaacaa caattgcgtt tcaccttcgc taatcagcac atcaacaaaa
    10621 cggcggataa gctggcgatt gagcggatca agcccctgta gtggttcatc gagaataagc
    10681 aacgtcggat gtttcaccag tgcacggacg atcagcgcca ggcgttgttg cccccaggaa
    10741 agactatgga acggagcgtc agccgtgcgt ttatcaatgc cgagaatatc cagccactgc
    10801 tgcaccagtt tttgctggcg atccgaaacg gcctgataaa tgccaatcga atcaaaatag
    10861 ccagaaagga tcacattacg cacggtagtg ctgacccggt aatccagatg caaactactg
    10921 ctgacgtaac cgatatgctt tttgatatcc cagatggttt cgccgctgcc gcgacgtcgt
    10981 ccgaaaagcg tcaaatcgtt gctgtaacct tgcggatgat cgccagtaat caggcttaat
    11041 aacgtcgatt ttcccgcgcc atttggcccg acaatttgcc agtgctcgcc tggattcacc
    11101 tgccagctaa ggttattaag aatggggcga tcgttataag aaaccacgcc gttgttcagc
    11161 acaatgcgcg gttcgttggc gggtaaggcg tgacgtgctg aaggttcatc cggctccggc
    11221 agttgtacac cttcaagctg ttcactatgc gccagttgcg cgacgagtgc ttgttggagc
    11281 agttcctctt tagcgctagt ttccgctaac gtgcaatccg ccagcacgcc agcaaactgg
    11341 acaaactccg ggatctcatc gaagcgattg agcaccagta ccagagtaat accggactga
    11401 tgtaacgagg cgagtcgctc agccagctgc tgacgtgagg caacatccag gccatcgaac
    11461 ggctcatcaa gaatcaacaa gtcaggctcc gacatcagcg cctgacacag cagggttttt
    11521 cgtgtctcgc cagtggaaag gtatttaaag cgtcggtcga ggagggcggt aataccgaac
    11581 tgctgcgcca gttgcatgca acgcggagta tcctttactt catcctgaat gatctcagcc
    11641 gtggtacgcc cggtgtcatc ttcgccagga ctaagcatat cggtgttatt ccgctgccat
    11701 tcgtcgctga cgagcttttg caattgctcg aaggagagac gagtgatgtg ggaaaactgg
    11761 ctttgccgtt cacctttcaa aagcggaagt tcccccgcca gcgcgcgggc cagggccgat
    11821 ttcccgcttc cattcgaacc gacaaacgcc caactatcac ccgcgtttaa cgttagctga
    11881 ggcaattgca gcgtttttgt gtcgctaaga cgaaacgtgc cttgcaaaat ttgcaacgat
    11941 gacattttat atcccatttt gtgcagcgat tactgacagg gatacgtgtt tcataacaaa
    12001 ttgtcaacac gcttagcata gcgtggcgat aatcacgctg tcggcattaa agtaggccgt
    12061 gacattctgt ccttgctgaa gagaagtcgc ttcatttacc ggcactgtgg cgcacagtgt
    12121 ttgcccgtcg ggtagcgcca ttaatacttc gcactgctct gcgccgcgct caatatgact
    12181 aataataccc ggtaattggt tgtcggcgtt ttgtgcgacc gcttcgtcct gagtaatgcc
    12241 tacccacggc gcttttagca atatcaacac ttctttgcct tcatccagcc ccagacgtgc
    12301 gccgctttgt gcggtaattg cgactttcag gcgtgttttt ccatcagcca gtaagacatc
    12361 aacatgctgt tgaacgtcat catgatcgcg ggcggtgatg gtaccgaacc actggttacg
    12421 ggcgctggtt tgcagtgaaa aacgtgagat cgcggccagc aggctgttca gcggcagggc
    12481 gtcatcgtca cttaacacat caaaggcttt ttgctggatt tgcgccagta agtcatagag
    12541 ctgaatcagt cgctgaccat agcgggtcag tactgcgccg cctccacctt taccgcctgt
    12601 tgcgcgctca accagaatat gctcacttaa ctgattcatc tcgttaatgg catcccaggc
    12661 gcttttatag ctgataccgg catctttcgc tccctggcta atggaaccgg aaagcgcaat
    12721 gtgttttagt agcgaaatgc ggcgcgggtc ggcgaataat ttttgttgga gcttaagggt
    12781 gagaaggatt tcggcctgca taacaatgtc ctggcaaaag tcttattgtg acggaaaacg
    12841 aacgccacgc aaagctgacc gcacaaaagg ggagtgcttt tctgtgctta gcggttagaa
    12901 tagtctcatg actatatctg gagttgacca tgttagagtt attaaaaagt ctggtattcg
    12961 ccgtaatcat ggtacctgtc gtgatggcca tcatcctggg tctgatttac ggtcttggtg
    13021 aagtattcaa catcttttct ggtgttggta aaaaagacca gcccggacaa aatcattgat
    13081 tccctgaatg cccgcttagt cgggcatttt ctttttctca acttcctgct tttcctgccg
    13141 atattttttc tcatctacct cacaaaggtt agcaataact gctgggaaaa atccgaactt
    13201 gccgttatat tgacgcctac ataacgttac attaaggggt taccaatggc tcgtaaatgg
    13261 ttgaacttgt ttgccggggc ggcactctct ttcgctgttg ctggcaatgc actggcagat
    13321 gaagggaaaa tcacggtgtt cgccgccgca tcactgacta acgcaatgca ggacattgct
    13381 acgcagtata aaaaagagaa aggcgtggat gtggtttctt ctttcgcttc gtcatctact
    13441 ctcgcccgtc agattgaagc gggagcccct gcggatctgt ttatttctgc cgatcagaaa
    13501 tggatggatt atgcggttga taaaaaagcg atcgatacag ctacgcgtca gacactgctc
    13561 ggcaatagcc tggtcgttgt agcaccgaaa gccagtgagc agaaagattt caccatcgac
    13621 agcaaaacca actggacttc actgctgaat ggcggtcgcc tggcggttgg cgatccggaa
    13681 catgtccctg ctggcattta tgcaaaagaa gcactgcaaa aactgggcgc atgggatacg
    13741 ctctccccga aactggctcc ggcggaagat gttcgtggcg cgctggcgtt ggtcgaacgc
    13801 aatgaagcgc cgctgggtat tgtttacggt tctgacgcag ttgccagcaa aggggtaaaa
    13861 gtggttgcca ccttcccgga agattcacat aaaaaagtgg aatatccggt tgctgttgtg
    13921 gaagggcata ataatgcgac agtgatagca ttttatgatt atctgaaggg gccgcaggct
    13981 gcggaaatct ttaaacgtta cggatttaca accaagtaat gatactgacc gatccagaat
    14041 ggcaggcagt tttattaagc ctgaaagttt cttccctggc tgtgctgttt agcctgccgt
    14101 ttgggatctt ttttgcctgg ttactggtac gttgcacgtt tccgggcaaa gctctgctcg
    14161 atagcgtact gcatctaccg ctggtgttac cgcccgtggt cgtcggttac ttattattag
    14221 tttcgatggg acggcgcgga tttatcggtg aacgtctgta tgactggttt ggtctttctt
    14281 tcgcgtttag ctggcgcggc gcggttctcg ctgccgccgt catgtcgttt ccgctgatgg
    14341 tgcgggcaat tcgtctggcg ctggaagggg ttgatgtcaa actggaacag gccgcaagaa
    14401 cactgggtgc cgggcgctgg cgcgttttct ttactatcac gttaccgctg accttaccgg
    14461 gcattattgt tggtacggta ctggcttttg ctcgttctct cggtgagttt ggtgcaacca
    14521 tcacctttgt gtcgaacatt cctggtgaaa cgcgaaccat tccttctgcc atgtataccc
    14581 tgatccagac tcccggcggg gaaagtggcg cggcgagact gtgcattatt tctattgcac
    14641 tggcgatgat ctccctgttg atttcagaat ggctggccag aatcagccgt gaacgggcgg
    14701 ggcgctaatc atgctggaac tgaatttttc ccagacgttg ggcaaccatt gcctgaccat
    14761 taatgaaacg ctgcccgcca atggcatcac tgctatcttt ggcgtctccg gtgccggaaa
    14821 aacttcgctg attaacgcca tcagtggact gacgcgcccg caaaaagggc ggattgtcct
    14881 caatgggcgg gtactaaatg atgccgaaaa aggtatctgc ctgacgccag aaaagcgtcg
    14941 cgttggctat gtttttcagg atgcacggct gttcccgcat tacaaagtgc gtggcaatct
    15001 gcgctacggc atgtcgaaaa gtatggtcga tcagttcgat aagctggtgg cgcttttagg
    15061 cattgaaccg ttgcttgacc gtttaccagg cagcctgtcc ggaggcgaaa aacagcgcgt
    15121 ggcgattggt cgggctttgc tgacagcacc ggaattgctg ttgctggatg aaccgctggc
    15181 gtcactggat attccgcgta aacgcgaact gttgccttat ctgcaacggc tgacacggga
    15241 aatcaacatt ccgatgttgt atgtcagcca ttcgctggat gagatcctcc atctggcaga
    15301 cagagtgatg gtactggaaa acggtcaggt gaaagccttt ggcgcgctgg aggaagtgtg
    15361 gggcagtagc gtgatgaatc cgtggctgcc gaaagagcaa caaagtagca ttctgaaagt
    15421 gacggtgctt gagcatcatc cgcattacgc gatgaccgcg ctggcgctgg gcgatcagca
    15481 tttgtgggtc aataagctgg acgaaccgct gcaagctgcg ctacgtatcc gcattcaggc
    15541 ttccgatgtt tctctggttt tacaaccgcc gcagcaaacc agcattcgta acgtattgcg
    15601 ggcaaaagtt gttaatagtt atgacgacaa cggccaggtg gaagtggagc tggaagtcgg
    15661 cggtaaaacg ctgtgggcgc gtatcagccc gtgggccagg gatgaactgg cgatcaaacc
    15721 aggcctgtgg ctgtacgcgc aaattaaaag tgtgtcgata accgcctgat taaatcaggt
    15781 ggctataaat gaaccgggca atgctgtcgg tggtgttatc accaatcaca atgttggcgc
    15841 gcgcttttac cgcatcatcg gcgttgccca tcgccacgcc tgtaccagcg gcttccagca
    15901 tactgatatc attaaagtta tcgccgaatg ccacgacgtt ttccatcgac caaccttgtg
    15961 cctcaaccca tttcgccaaa cgtttacctt tgctgttgcc gccgcgtgca atatcaacct
    16021 gatcgtgcca ggaccattca cactcaagtc ccagttcatg ttcgacatgc ttaccaaaat
    16081 gctgcaattg cggcaggtca tcgtgcgtca gggcgaactt ccatacggcg ttaacttgtt
    16141 gcgccgtttc agccagagaa gcgacttgtg tgaaagtcgg acgctgttcc ggcggcaggg
    16201 tttgcgccca gttagacgtg cgaatgacat gcccggtcgg gtgctcatag accattgcat
    16261 catcgacata catcagaccg tgaatgtggt gttcattcag catctcaatg agttgcaggg
    16321 ctttattaac gggcattggg tccgcttcca gcactgtttt tgcatgataa tcatacaaat
    16381 aggtgccatt acagcaaata gcaggtgtat ccagcgccag cgcctgataa aaaggatgaa
    16441 tagcgacgtg atggcgacct gtgacgatga ttaattgata gcctgcttcg cgagcgcggg
    16501 ccagggcttc tatcgatgat ggaagcaagg tctttttcgg ggtcaataag gtgccgtcta
    16561 agtcgagagc aatcacgcgt gtggtcatgg cgtattccag attaaggtta agaattttct
    16621 tctgcgcgaa tggtacaccg ataccactat cggacaaaat tctgcgtttt aattcagcat
    16681 tcaccgccaa aagcgactaa ttttagctgt tacagtcagt tgctaaatgc aaaggagcat
    16741 tcatgaagca aacagtttat atcgccagcc ctgagagcca gcaaattcac gtctggaacc
    16801 tgaatcatga aggcgcactg acgctgacac aggttgtcga tgtgccgggg caggtgcagc
    16861 cgatggtggt cagcccggac aaacgttatc tctatgttgg tgtgcgccct gagtttcgcg
    16921 tcctggcgta tcgtatcgcc ccggacgatg gcgcgctgac ctttgctgca gagtctgcgc
    16981 taccgggtag cccgacgcat atttccaccg atcaccaggg gcagtttgtc tttgtcggtt
    17041 cttacaatgc gggtaacgtg agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg
    17101 tcgatgtggt cgaggggctg gatggttgcc attccgccaa tatctcaccg gacaaccgta
    17161 cgctgtgggt tccggcatta aagcaggatc gcatttgtct gtttacggtc agcgatgatg
    17221 gtcatctggt ggcacaggac cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc
    17281 atatggtatt ccatccaaac gaacaatatg cgtattgcgt caatgagtta aacagctcag
    17341 tggatgtctg ggaactgaaa gatccgcacg gcaatatcga atgtgtccag acgctggata
    17401 tgatgccgga aaacttctcc gacacccgtt gggcggctga tattcatatc accccggatg
    17461 gtcgtcattt atacgcctgc gaccgtaccg ccagcctgat taccgttttc agcgtttcgg
    17521 aagatggcag cgtgttgagt aaagaaggct tccagccaac ggaaacccag ccgcgcggct
    17581 tcaatgttga tcacagcggc aagtatctga ttgccgccgg gcaaaaatct caccacatct
    17641 cggtatacga aattgttggc gagcaggggc tactgcatga aaaaggccgc tatgcggtcg
    17701 ggcagggacc aatgtgggtg gtggttaacg cacactaacc gctgatttac ccggcgcagt
    17761 ctctcctgcg ccggtgtatt aacctatctc ctgtaacgca tgtctctggc gttcgacgat
    17821 attggtccac aaattgtctt tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg
    17881 ttcagctgcc ggagaaagca cggcatcttt gcggcgaata atccccaatg tccgacgtat
    17941 aacgggttcc accagtggga taccaataat cgaagaatag ggcgcatggg gcattgccag
    18001 accaggaagc gccgaaatcc ccagtcctgc ctccaccagt cctaatgacg tcgaaagatg
    18061 acgcacttcg taaaaccagt ccagcttcca gggcttgtcg gctagctgtt gttctatcaa
    18121 cagtcggttg ccgctggagg agcgtacgcc aatcattttg taacccacta actcctgcca
    18181 ttctacaagc tgctttttga ccaatggatg gtcacgccga caggccagca cgaacggctc
    18241 gttgaccagt ggggtaaaat cgatggatga atttgtgacg ttgttcatgt ttatgccaaa
    18301 gtctgattcg ttacacagca ccgattccat gcaattattc gtgccttgtt ccagaatccg
    18361 cactttaata ttgggataca gctcattaaa tttaccgatt gccagcggta aaaaataaaa
    18421 tactgcggtc ggaatacacg ctaatgtcac cataccacga tgataagcat tcatatcacg
    18481 aatattaaaa agcgtttcat cgaatttctt tattaattcc cgcgcctccg ggagcaatct
    18541 ttttcctact tttgttaacg taaccttgcg agttgtacgc tcaaatagtt ggatatgtaa
    18601 atcttcctcc atctttttta ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt
    18661 agcggcattg ttaaatgaac tggactctgc cagtatgaca aatgccttca tacttgataa
    18721 ttcatgcttc attattactc cggaaaatgg aagcgtcgat tttgggtggc tgaccgttaa
    18781 aaattttaac tgcatttaac caacttaaat taatgaaaaa atgttattaa tcgttgagct
    18841 aaagtcatta gagatgcttt gcccttaatg taaccatatc gcaataagtt atgtttttaa
    18901 attgagggca ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg
    18961 ggcgcgttcc tgttagcgga acatctaccc gaagatcaaa cgcagcgcga taaaatattg
    19021 atggcaatta tgggttccgg taacgatctg gaaattgacg gtattggcgg cggtaatccg
    19081 ctgaccagta aagtcgccat tattagccgc tccagcgatc cgcgtgcaga tgtcgattat
    19141 ctgtttgctc aagttatcgt ccatgagcaa cgtgtcgata ccacgcctaa ctgcggcaat
    19201 atgctgtctg gcgttggggc attcgccatt gaaaatggtt tgattgcagc gacttcgcca
    19261 gttacccgcg tacgtatccg caacgtcaat acgggtacgt tcaccgaagc tgatgtgcaa
    19321 acgccaaatg gtgttgtcga gtacgagggc agcgccagaa ttgacggcgt accgggtact
    19381 gccgcaccgg ttgcgctcac tttcctgaat gccgccggaa ccaaaaccgg aaaagtcttc
    19441 ccgacggata atcagattga ttattttgac gatgtcccgg tgacctgtat cgatatggcg
    19501 atgccagttg tcattattcc tgctgaatat ctgggaaaaa caggctatga attaccgacg
    19561 gagttggatg ccgacaaagc attattagct cgcattgaat ctatccgtct acaagcgggt
    19621 aaagcaatgg gcttaggcga tgtcagtaat atggttatcc ctaaacctgt acttatttct
    19681 ccagcgcaga aaggcggggc aattaatgtg cgttatttta tgccgcattc ttgccatcgc
    19741 gctttggcga taaccggtgc tattgctatt tccagtagtt gtgcattgga aggcaccgtc
    19801 acccgacaaa tcgtcccttc tgtaggatac ggcaatatca atattgaaca ccccagtggt
    19861 gcgctcgacg ttcatttaag taatgaaggt caggatgcca cgacgttacg cgcatctgtt
    19921 attcggacga ccagaaaaat attttccggt gaagtttatc ttccctgaaa aaattcgttg
    19981 tcaggataag gacaatcaat aaaggacttc tgtatgagtc atacagaaag aacaggatat
    20041 taaatgaata agaaatcgtt atggaagcta attctgatat tagcaatccc atgtattatt
    20101 ggctttatgc cagctccagc aggattaagc gaactggcgt gggtgctttt tggtatttac
    20161 ctggcggcca ttgtggggct ggttatcaag cctttcccgg aacctgtcgt actgttaatt
    20221 gccgtcgccg catcgatggt agtggtgggt aacttatcag gtggggaatt taaaaccacc
    20281 gccgtattaa gcggttactc ttccggtacc acctggctgg tgttttctgc gtttacttta
    20341 agcgccgcgt ttgtaaccac aggcttaggt aaacgtattg cctatctgct gattggtaaa
    20401 attggtagca ctaccctggg tctgggttac gttacggtat ttctcgatct ggtactggct
    20461 ccggcaacac cgtctaacac cgcgcgtgcg ggcggcattg tgttaccgat catcaacagc
    20521 gtggcggtgg ctttggggtc agaaccggaa aaaagtccgc gtcgtgtcgg acattacctg
    20581 atgatgtcca tttacatggt caccaaaacc accagctata tgttctttac cgcaatggcg
    20641 gggaacattc tggcgctgaa aatgatcaac gacattctgc acctgcaaat tagctggggt
    20701 ggatgggcgc tagccgccgg attgcctggc atcattatgc tgctggtcac cccgctggtg
    20761 atttacacca tgtatccacc agaaattaag aaggtggata acaaaaccat cgctaaagcg
    20821 ggccttgccg aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg tgtctttgtg
    20881 ctggcgctgc tgggctggat tttcagtaag tctctggggg ttgatgaatc caccgtggca
    20941 atcgttgtta tggcaactat gctgctgctg ggtatcgtta cctgggaaga cgtggttaaa
    21001 aataaaggcg gctggaatac cttaatctgg tacggcggta ttatcggctt aagctcctta
    21061 ttatcgaaag ttaaattctt cgaatggtta gctgaagtct ttaaaaataa cctggcattt
    21121 gatggtcacg ggaacgttgc tttcttcgtt attacctaga ttctacgtca gtacttcaaa
    21181 aagcataatc aaagccttga taaatatgca ttccttcgaa attcagcttt cacccattgg
    21241 gtgaaagaaa agtgctc
//
LOCUS       DAOTRK010000080        21124 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.080, whole genome shotgun sequence.
ACCESSION   DAOTRK010000080 DAOTRK010000000
VERSION     DAOTRK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.080"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..204)
                     /locus_tag="QIE41_002821"
     CDS             complement(<1..204)
                     /locus_tag="QIE41_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3531693.1"
                     /translation="MCYNGLNNMFFPLCQINNNHSVTSPSHTKKTKSDNYRKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKM"
     gene            complement(382..2031)
                     /gene="actP"
                     /locus_tag="QIE41_002822"
     CDS             complement(382..2031)
                     /gene="actP"
                     /locus_tag="QIE41_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDS3531694.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLLTAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSVTDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2028..2342)
                     /locus_tag="QIE41_002823"
     CDS             complement(2028..2342)
                     /locus_tag="QIE41_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDS3531695.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2569..4527)
                     /gene="acs"
                     /locus_tag="QIE41_002824"
     CDS             complement(2569..4527)
                     /gene="acs"
                     /locus_tag="QIE41_002824"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDS3531696.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLIEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVISDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4920..6356
                     /gene="nrfA"
                     /locus_tag="QIE41_002825"
     CDS             4920..6356
                     /gene="nrfA"
                     /locus_tag="QIE41_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDS3531697.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQKAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6401..6967
                     /gene="nrfB"
                     /locus_tag="QIE41_002826"
     CDS             6401..6967
                     /gene="nrfB"
                     /locus_tag="QIE41_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDS3531698.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6964..7635
                     /gene="nrfC"
                     /locus_tag="QIE41_002827"
     CDS             6964..7635
                     /gene="nrfC"
                     /locus_tag="QIE41_002827"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDS3531699.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7632..8588
                     /gene="nrfD"
                     /locus_tag="QIE41_002828"
     CDS             7632..8588
                     /gene="nrfD"
                     /locus_tag="QIE41_002828"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDS3531700.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLLLWLAKIFEHDLLALQQRWLPKLGFVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8608..10326
                     /locus_tag="QIE41_002829"
     CDS             8608..10326
                     /locus_tag="QIE41_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDS3531701.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALGSLLRGEFDGASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFTLFALISLASLALYGW
                     RARDGGPAVHFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10319..10702
                     /gene="nrfF"
                     /locus_tag="QIE41_002830"
     CDS             10319..10702
                     /gene="nrfF"
                     /locus_tag="QIE41_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDS3531702.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQERALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10699..11295
                     /gene="nrfG"
                     /locus_tag="QIE41_002831"
     CDS             10699..11295
                     /gene="nrfG"
                     /locus_tag="QIE41_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDS3531703.1"
                     /translation="MKPPQIPVKMLTTLTILMVFLCVGSYLLSPKWLAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRTNPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11637..12950
                     /gene="gltP"
                     /locus_tag="QIE41_002832"
     CDS             11637..12950
                     /gene="gltP"
                     /locus_tag="QIE41_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDS3531704.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13029..13718)
                     /locus_tag="QIE41_002833"
     CDS             complement(13029..13718)
                     /locus_tag="QIE41_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDS3531705.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13812..15959)
                     /gene="fdhF"
                     /locus_tag="QIE41_002834"
     CDS             complement(13812..15959)
                     /gene="fdhF"
                     /locus_tag="QIE41_002834"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15540..15542),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDS3531706.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16157..17623)
                     /gene="mdtP"
                     /locus_tag="QIE41_002835"
     CDS             complement(16157..17623)
                     /gene="mdtP"
                     /locus_tag="QIE41_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDS3531707.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMHWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            complement(17620..19671)
                     /gene="mdtO"
                     /locus_tag="QIE41_002836"
     CDS             complement(17620..19671)
                     /gene="mdtO"
                     /locus_tag="QIE41_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDS3531708.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMALRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19671..20702)
                     /gene="mdtN"
                     /locus_tag="QIE41_002837"
     CDS             complement(19671..20702)
                     /gene="mdtN"
                     /locus_tag="QIE41_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDS3531709.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAGLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGT
                     SAVANLEPQ"
     gene            complement(20721..20996)
                     /locus_tag="QIE41_002838"
     CDS             complement(20721..20996)
                     /locus_tag="QIE41_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531710.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLLWLAFF"
BASE COUNT         4776 a         5582 c         5733 g         5033 t
ORIGIN      
        1 cattttctga atcaagccta tcactttttc atgatcatct attttgaaaa gagagagggc
       61 tttattgtca attaacgtgt ttttatgatg ttttctgtaa ttatctgatt ttgttttctt
      121 tgtatgtgat ggacttgtga cagagtggtt attgttaatc tggcaaagag ggaaaaacat
      181 attatttaaa ccattgtagc acataaaata tccttataat taacaagaaa gaaaaggcat
      241 attctctgca ttatcatttt ctacgactgt caaaaatcgc tcatttttta atgcgtttat
      301 ttgtttaata ttatgggaaa aggtgatgca ttttgcagag gaagagtatt ccccggtcag
      361 gcgaccgggg aaggggagag attaatgcgc gcggccttgc tcaacgccaa agccggtctg
      421 ggagcggata aactgcgcac ggaacagttc acgctcacgc gcgccttccg ctgagttatc
      481 ggttaccgag aagaaccaga tgccgaggaa tgccacgctg atagagaaca gcgccgggta
      541 ttcatacggg aagatggctt tttcgtgacc gaggatctgt acccaaatcg tcgggccgag
      601 gatcatcagt accactgcgg taagcagccc cagccagccg cccatcattg cgccacgcgt
      661 ggtcagtttt gaccagtaca tggaaagcag aatgattggg aagttacagc tcgctgcgat
      721 ggcaaacgcc agccccacca taaaggcgat gttctgattc tcaaacagca cgccgaggat
      781 aatggcaatc acgcccaaaa tcaggacggt gattttcgat acccgcagct cttcacgttc
      841 agtcgcgcct tttttgaaga cgttagcgta caggtcgtga gacaccgccg atgcgcccgc
      901 cagcgtcaga cccgcaacca ccgcaagaat ggtggcgaaa gcaaccgctg aaataaagcc
      961 gaggaacagg ttgccaccca ctgcattcgc caggtgaact gccgccatgt tgttaccacc
     1021 aatcagatgg cccgccgcgt ctttatattc cggattcgca ccaaccagca tgatcgcgcc
     1081 gaagccgata ataaaggtca gaatatagaa gtagcccata aatccggtag cgtagaacac
     1141 gctcttacgc gcttcgcggg catcgctgac cgtaaagaag cgcatcagaa tgtgcggcaa
     1201 gccagccgta ccaaacatca gtccgagccc cagcgacagt gctgagatcg gatctttcac
     1261 cagcccgcct ggcttcatga tgtcgacacc cttcgggtgt accgccatcg cttcgctgaa
     1321 cagattgttg aagctaaagc cgacgtgttt catcaccata aaggccataa agctggcacc
     1381 gaacagcaac agcacggctt tgataatctg tacccaggtg gtcgccagca tgccgccgaa
     1441 caggacgtac atcatcatca gcacgccgac cagcactacc gcaatgtgat agttaaggcc
     1501 aaacagcagc tcgatcagtt tacctgcgcc taccatctga gcgataaggt aaagcgccac
     1561 caccaccaga gaaccacagg ccgaaagaat acgaatcggc ccttgtttca ggcggtaaga
     1621 ggctacatcg gcaaaggtgt agcgtcccag gttacgcaga cgttcggcaa tcaggaacag
     1681 aatgattggc cagcccacca ggaagcccaa cgagtagatc agcccgtcat agccggaggt
     1741 aaacaccagc gcggagatcc ccaagaatga ggcggcggac atatagtccc cggcaatcgc
     1801 cagcccgttc tgaaaaccgg tgatattgcc gcctgccgtg tagtagtcat tacgcgaacg
     1861 tacgcgtttt gacgcccagt aggtaatacc gagcgtaaac acgacgaaaa tcaggaacat
     1921 aataatcgcc tgccagtttg ttggctggcg ctctacggcc ccgctaatag catccgcggc
     1981 gttagctgcg aaagggagtg tggcggcaag cgccgtcaga actcttttca tgatgcttgt
     2041 acctcatgca ggacttcatt attaagacgg tcgaattcgc cgttcgcccg ccagatgtag
     2101 ataccagtga gaacaaagga gatcacaatt accccaacac caatcggaat accgcgtgtg
     2161 acgctggtgt tcggattcag cggagtgccc agccagccgg gcgcgaaggc gatcagtaaa
     2221 ataaagccga tataaactgc cagcataata atcgacagga tggtggcaaa ccgttgccgt
     2281 ttttcgacta actccctgaa atgcgcattg tcttctatcc gctgataaat cgtaccattc
     2341 atcacagatt ctccagaggt tccccgtcgt ctttcacgcc gcaggttcgt cagccgcact
     2401 cgcctccccc agtcacatag ttttctatgc tcctggggag atgctcgctt gcttcctgcc
     2461 tgcaacgcga aatacttagg ggaattttta tatttgccct ttcccactat aaagaaaggg
     2521 gcgtgtagac cggataaggc gttaaacgcc gcatccggca attgtgggtt acgatggcat
     2581 cgcgatagcc tgcttctctt caagcagctt ctcgaccacg ccaggatcgg caagcgtcga
     2641 ggtatcgccc aggttgctgg tatcgcccgc cgcaattttg cgcagaatac ggcgcataat
     2701 tttgccggag cgggttttag gcagggaatc ggtccagtgc agcacgtctg gcgtcgccag
     2761 cgggccaatc tctttacgca cccagttgcg gacttctgcg tacagttctg gtgacggttc
     2821 ctccccgtga ttaagcgtga cgtaggcgta gatcgcctga cctttaatat tgtgcggaat
     2881 accgacgaca gcggcttcgg caatcttcgg atgtgccacc agcgccgact caatctctgc
     2941 cgttcccaga cggtgaccgg agacgttcag cacgtcgtcc acgcgcccgg tgatccagta
     3001 atagccatct tcatcgcgac gcgcgccgtc gccgctgaaa tacatatttt tgaaggtgga
     3061 gaagtaggtc tgctcaaaac gttcgtgatc gccaaacagc gtacgcgcct gacccggcca
     3121 ggagtcggag atcaccagac tgccttcggt ggccccttcc agcgggttac cttcgttatc
     3181 gaccagcgcc ggttgcacgc cgaagaacgg acgtgttgcc gaaccggctt tcagctcggt
     3241 agcgccaggc agcggggtga tcatgaaacc gccggtttcg gtctgccacc aggtatcgac
     3301 caccgggcat ttctcgttgc cgattttttt ccagtaccac tcccacgctt ccgggttgat
     3361 tggctcgcct acggaaccga gaatgcgcag cgacgaacgg tcagtgcctt cgatcgcttt
     3421 atcgccttcc gccatcagcg cgcggatcgc cgtgggcgcg gtatagagaa tattgacctg
     3481 atgcttgtcc accacctgcg ccatacgggc aggcgtcggc cagttcggta cgccttcaaa
     3541 catcagcgtg gtcgcgccgc aggccaacgg gccgtacagc aaataactgt gtccggtaac
     3601 ccaacccaca tcagcggtgc accagtagat atcgcccgga tgataatcaa agacatattt
     3661 aaaggtcagc gccgcataca ccagataacc gccggtagtg tgcagcacgc ctttcggctt
     3721 cccggtagaa ccggaggtgt agagaataaa cagcggatct tcggcgttca tctcttccgc
     3781 ctggtgctga tcgctggctt gctcaatcag gtcgtgccac cacagatcgc gcccttcctg
     3841 ccagtcaatt ttcccgccag tacgcttcag taccaccaca tgttcaatgc tggtgacgtt
     3901 cgggtttttc agcgcgtcat caacgttttt cttcagcgga atactacgcc cggcgcgcac
     3961 gccttcgtcg gaagtgatca ccaaccgtga gttagaatcg ataatgcgcc cggcaacggc
     4021 ttccggcgag aagccgccga aaattaccga atgcaccgcg ccaatgcggg cgcaggccag
     4081 catcgcgacc gcggcttccg gcaccatcgg catataaatc gccaccacat cacctttttt
     4141 aatgcccagc tcgagcaggg tattggcgaa gcggcagacg tcgcggtgca gctctttata
     4201 gctgatatgt ttgctctggc tggcgtcgtc gccttcccag atgatggcgg tacgatcgcc
     4261 gttttcttgc agatggcggt caaggcagtt tgccgccaga ttcagcgtgc cgtcctcgta
     4321 ccatttaatg gacacattac cgggggcaaa ggaggtgttt ttcaccttct ggtaaggttt
     4381 gatccagtcg agaatttttc cctgttcgcc ccagaaggta tctggtgcgt taatagattg
     4441 ttgatacatc gcctcgtact gctgagggtt tatcaggcaa cggtctgcga tgttggcagg
     4501 aatggtgtgt ttgtgaattt gactcatgct tttgttctcc ttgtaggatg ttaataatat
     4561 gtggcataag agttaaatgt aggggtattg gcagttttgt ttagtatttg ggcgacagat
     4621 cacgcaaaag tagaattgtg caaataaacg gcagggtaat ttttgaaggt cagcaacaaa
     4681 agttgattaa ttctttgagg aacatgcagt tatgcatgct gttgaaaaag aggaagatac
     4741 tgactaactc taaagtggta ttttacatgc acttacaatt gattaaagac aacattcaca
     4801 gtgtggttat ttgttacaca taggggcgag caatgtcatg acagtgtagg tgcggttact
     4861 gtcgtgaaaa acaataaaaa ccgccattgc aacaatggcg caattcggat gaagccccta
     4921 tgacaaggat aaaaataaac gcacgccgta tcttcagctt attgattcct tttttctttt
     4981 tcacttctgt tcacgctgaa caaacggcag ctcccgcaaa acctgtaact gttgaagcga
     5041 agaatgaaac ctttgccccg caacatcccg atcaatatct ctcctggaaa gccacctcgg
     5101 aacagtcaga gcgtgttgac gccctggcgg aagatccacg gctggtgatc ctgtgggcgg
     5161 ggtatccctt ctcgcgcgat tacaacaagc cgcgtggaca tgcctttgct gtgaccgatg
     5221 tgcgtgaaac cctgcgcacc ggtgcgccga aaaacgctga agatggtccg ctaccgatgg
     5281 catgctggag ttgtaaaagc ccggatgtgg cgcgtctgat ccagaaagac ggcgaagatg
     5341 gctacttcca cggaaaatgg gcgcgcggcg gcccggaaat cgtcaacaac ttaggttgtg
     5401 ccgattgcca taacaccgcc tcaccagagt tcgccaaagg caaaccggag ttaacccttt
     5461 cccgtccgta tgcggctcgc gcgatggaag ccattggtaa accttttgag aaagccggac
     5521 gtttcgacca gcaatcgatg gtttgcggtc agtgccatgt ggagtattac ttcgacggca
     5581 aaaacaaagc ggttaaattc ccgtgggatg acggcatgaa agtcgaaaat atggagcagt
     5641 attacgacaa aattgccttc tctgactgga ctaactccct gtcgaaaacg ccaatgctga
     5701 aagcgcagca cccggaatat gaaacctgga cagcgggcat tcatggcaaa aacaacgtga
     5761 cctgtatcga ttgccatatg ccaaaagtgc agaacgccga aggcaaactc tacaccgacc
     5821 ataaaattgg taatccgttt gataacttcg cccagacttg tgcgaactgc catacccagg
     5881 acaaagctgc cttgcaaaaa gtggtcgcgg aacgtaagca gtcgattaac gacctgaaaa
     5941 tcaaggttga agatcaactg gttcacgctc acttcgaagc gaaagcggcg ctggatgcag
     6001 gcgcgacgga agccgaaatg aagccgattc aggacgatat ccgtcatgcc cagtggcgtt
     6061 gggatctggc gatcgcttcc cacggcattc atatgcacgc accggaagaa ggtctgagga
     6121 tgctcggtac ggcgatggat aaagcggcgg atgcgcgcac taaactggcg cgactgctgg
     6181 caaccaaagg catcacccat gaaatccaga tcccggatat ctcgaccaaa gagaaagccc
     6241 agaaggccat tggcctgaac atggaacaaa tcaaggccga gaagcaggac ttcatcaaaa
     6301 cggtgatccc gcagtgggaa gagcaggcac gtaaaaacgg tctgttaagc caataacccc
     6361 gttccgcctc gcaaggggcg gaaaacacaa tggagtgaat atgagcgtat tacgttcgtt
     6421 attaactgcc ggggtgctgg cgtcgggcct gttgtggagc ctgaacggaa ttaccgctac
     6481 ccctgcggcg caggcatctg gtgatcgcta cgaagttacc cagcagcgta acccggatgc
     6541 cgcctgtctg gactgtcata agccagatac cgaaggtatg catggcaaac atgcttccgt
     6601 tatcaacccg aataacaaac tgccggtcac ctgcaccaac tgtcacggcc agccatcacc
     6661 gcaacaccgc gaaggggtga aagatgtgat gcgctttaac gagccgatgt acaaggttgg
     6721 agagcagaac agcgtctgta tgtcctgcca tctgccggaa cagttgcaaa aagcgttctg
     6781 gccacacgat gtccacgtaa ccaaagtggc gtgcgccagc tgccattccc tgcatccaca
     6841 gcaagatacg atgcagacgt taagtgacaa aggacggatt aagatttgcg tcgattgcca
     6901 cagcgatcag cgcaccaatc cgaactttaa cccggcgtct gttccgttgc ttaaggagca
     6961 gccatgacct ggtcacgtcg ccagtttctc accggcgtcg gcgtgctggc agccgtcagc
     7021 ggcaccgcag ggcgcgtggt ggcgaaaacg ttgaatatca atggggtgcg ttacggcatg
     7081 gtgcatgacg agtcgttatg tatcggctgt accgcctgta tggatgcttg tcgggaagtg
     7141 aacaaggtgc cggaaggcgt ctcgcgcctg acgattattc gtagcgagcc gcagggcgaa
     7201 tttcctgatg tgaaatatcg cttcttccgt aagtcttgcc agcactgcga tcatgcgccg
     7261 tgcgttgacg tctgcccgac cggtgcgtct tttcgtgatg ccgctagcgg gattgtcgat
     7321 gttaacccgg atctctgcgt cggttgtcag tactgcatcg ccgcctgtcc gtaccgcgtg
     7381 cgctttatcc atccggtcac gaagacagcg gacaaatgcg atttctgccg taagaccaat
     7441 ttgcaggccg gtaagctgcc cgcgtgcgtt gaggcttgcc cgacgaaggc gctgacgttt
     7501 ggcaatctgg acgatcccaa cagtgagatt tcgcaactgc tgcgccagaa gcccacttac
     7561 cgctacaagc tggcgctggg aaccaaaccg aagctgtacc gcgtaccgtt taaatacggg
     7621 gaggtgagcc aatgacgcag acttccgcat ttcattttga atcgctggtg tgggactggc
     7681 cgattgccat ctacctgttt ttgattggta tttctgccgg tctggtgaca ctggccgtgc
     7741 tgttacgtcg cttctacccg caggcgggcg gtgcagacag tacgttgttg cgcaccacgc
     7801 tgattgtcgg gcctggcgcg gtgatccttg gtctgttgat cctcgtcttc cacctgacaa
     7861 gaccgtggac cttctggaag ctgatgttcc actacagttt tacctcggtg atgtcgatgg
     7921 gggtgatgct gtttcagctc tacatggtgg tgctgttgct gtggctggcg aaaatctttg
     7981 aacatgattt gcttgccctg caacaacgct ggttgccgaa gctggggttc gtgcaaaagg
     8041 ttctgagcct gctgacgccc gttcatcgcg gactggaaac attgatgctg gtgctggcgg
     8101 tgctgctggg ggcttatacc ggctttctgc tatcggcgct gaaatcgtat ccgttcctca
     8161 ataacccgat cctgccggtg ctgttcctct tctccggcat ctcgtccggt gcggcggtgg
     8221 cgctgatcgc catggcgata cgccaacgca gtaacccgca ttccacggaa gcgcactttg
     8281 tacaccgtat ggagatccct gtggtatggg gcgaaatctt cctgctggtg gcgttttttg
     8341 tcggtctggc gctgggcgat gacggtaaag tgcgtgcgct ggtggcggca ttgggcggcg
     8401 gtttctggac gtggtggttc tggcttggtg tcgccgggct ggggttgatt gtgccaatgt
     8461 tgctcaaacc gtgggtcaat cgcagttccg gcattcctgc cgtgctggcg gcgtgtgggg
     8521 ccagcctggt cggcgtgttg atgttgcgat ttttcattct ctacgccggg cagttaacgg
     8581 tggcgtaagc cagaacagag gtggtttctg gacgtattcc ttcctgaagt cggttttctg
     8641 gcgttgttgt taagtctcgg ggtcaacgtg ttgaccccgt tgacggcctt cgcgggagtg
     8701 cggttgcgct ggcctgccat gatgcgactc acttgcatcg gcattctggc gcagttcgcg
     8761 ctcctgctgc tcgcctttgg cgtactgacg tactgttttc tcatcagcga tttctcggtc
     8821 atttatgtcg cccaacatag ctacagcctg ctgtcgtggg aactcaaact ggcggcggtg
     8881 tggggcggtc atgaaggttc gctgctgctt tgggtgctgt tgctttccgc ctggagcgcg
     8941 ctgtttgcct ggcattatcg gcagcaaacc gatccgctat ttccgctgac gctagccgtt
     9001 ttatctctca tgctcgccgc actgctactg tttgtggtgc tgtggtccga tcccttcgtg
     9061 cggatatttc caccagcaat cgaaggccgc gatctcaatc cgatgctgca acatcccggt
     9121 cttatctttc atccgccgct gctttatctc ggctatggcg gtttgatggt ggcagcgagc
     9181 gtggcgctgg ggagtttact gcgcggcgag tttgatggtg ccagcgcccg aatttgctgg
     9241 cgctgggcac tacctggctg gagcgcatta acggcgggga tcatcctcgg ttcctggtgg
     9301 gcctattgcg aactgggctg gggcggctgg tggttctggg atccggtgga aaacgcctct
     9361 ttattaccct ggctttctgc cactgcgctg ctgcacagtt tgtccctgac acgccagcgg
     9421 gggattttcc gccactggtc gctgttactg gcgatagtca ctctgatgct gtcgctgctg
     9481 ggtaccttaa ttgtccgttc tggcattctg gtttcggttc atgcgttcgc gctggataac
     9541 gtccgcgccg tgccgttgtt caccctgttt gcactgatta gccttgcgtc tctggctctg
     9601 tatggctggc gagcgcggga cggtggcccg gcggtgcatt tttcggggtt atcgcgggaa
     9661 atgttaatcc tcgctacgct gttgctgttt tgcgcagtgc tattgatcgt gctggtggga
     9721 acgctttatc cgatgattta cggcctgctg ggctggggac gcctctccgt tggcgcgccg
     9781 tattttaacc gcgcgacgtt accgtttggt ctgttgatgc tggtggtgat tgtgctggcg
     9841 acgtttgtct ctggcaaacg cgtgcagctt ccggcgctgg tagctcatgc gggcgtgctg
     9901 ttatttgccg cggggatcgt ggtctccagc gtcagccgtc aggagatcag cctgaattta
     9961 cagccgggtc agcaggtgac gctggcagga tacaccttcc gttttgagcg cctcgatctg
    10021 caagccagag gcaattacac cagcgaaaaa gcgatagtgg cactgtttga ccatcagcaa
    10081 cgtattggtg aactgacgcc ggagcggcgt ttttacgaag cacgccgtca gcaaatgatg
    10141 gagccgtcaa ttcgctggaa cggcatccat gactggtatg cggttatggg ggagaaaact
    10201 gggccggatc gttacgcttt tcgtttgtat gtacaaagcg gtgtgcgctg gatctggggg
    10261 ggaggattgt tgatgattgc gggcgcattg ttaagcggat ggcgggggag gaagcgcgat
    10321 gaataaagga cttctcacgc tactgctgtt atttacctgt tttgcgcgcg ctcaggttgt
    10381 agacacctgg caattcgcca atccgcaaca acaggaacgg gcgttaaata ttgccagcca
    10441 gttacgttgt ccgcagtgcc agaatcagaa cttactggaa tccaatgcgc cggtggcagt
    10501 tagtatgcgc catcaggttt acagcatggt ggcggagggg aaaagtgaag tcgaaatcat
    10561 tggctggatg accgaacgct acggagattt tgttcgctat aacccaccgt taacgggtca
    10621 gacgctggta ttgtgggctc tgccagtggt gttgttactg ctgatggcac tgatcctctg
    10681 gcgagtgagg gcgaagcgat gaaaccgcct caaataccgg tgaaaatgct gacaacactc
    10741 acgatattga tggtatttct ctgtgtcggc agttatctgc taagtccaaa atggctggcg
    10801 gtgcgtgcgg agtatcagcg tcagcgcgat ccgctacatc agtttgccag ccagcaaacc
    10861 ccggaagcgc agcttcaggc attgcaggat aaaatccgca ctaatccaca aaacagcgaa
    10921 caatgggcgt tactgggcga gtattatctg tggcaaaacg attacagcaa ttcgctgctg
    10981 gcttaccgtc aggcgttgca actacgtggt gagaacgctg aactgtatgc ggcgctggcg
    11041 acggtgcttt attaccaggc tagccagcat atgaccgccc agactcgcgc aatgatcgac
    11101 aaagccctcg cgctggactc taacgaaatc accgctctga tgctgctggc ttccgatgcg
    11161 tttatgcagg cgaattacgc gcaagccatc gaactatggc aaaaagtgat ggatctcaac
    11221 tcaccccgga tcaacaggac acagctggtc gagtcgatta atatggcgaa attgttgcag
    11281 cggagatcag attaatcatc tggtgatgga gattatgtta ttgagctgca taaaaaataa
    11341 tcgaatgaac atatgccaaa aataatcacc aatcagtatt attgcagatt aaacaaataa
    11401 aaatctttcc ataacaaatg gttattcatt aatcctgcca taaactcgcg ttattatgca
    11461 ttaatgcagc gaaaagctct gttgttaaag ggttgcgcaa cataccgcgc aaatgatact
    11521 gatcataagc gttaaaaaaa tctacaaacc aacgcaacac aattcatgcc ctggcagtat
    11581 gtcacgttct cacgtttctg aacggggaac ggcgctccat tgaggaagtt attctgatga
    11641 aaaatataaa attcagcctg gcctggcaga ttctgtttgc tatggtgctg ggcattctcc
    11701 tgggaagcta cctgcactac catagcgaca gccgcgactg gctggtcgtc aatttgctct
    11761 ctccggcggg tgatatcttc atccatctga tcaaaatgat tgttgtgccg attgtgatct
    11821 ccacgctggt ggtgggtatc gcgggtgttg gtgatgccaa acagctcggg cgtattggcg
    11881 cgaaaaccat tatttacttc gaggtgatca ccaccgtcgc catcattttg gggatcactc
    11941 tggcgaacgt cttccagccc ggtgccgggg tggatatgtc gcagctggcg accgtcgata
    12001 tctcgaaata tcagagcact acggaagcgg tacaaagcag ttcccacggc attatgggca
    12061 cgattttgtc gctggtgccg acgaacattg tggcgtcgat ggcgaaaggc gaaatgctgc
    12121 cgatcatctt cttctcggtg ctgtttggtc tggggctttc ctccctgccc gcgacgcatc
    12181 gtgaaccgct ggtgaccgtg ttccgctcca tctctgaaac catgtttaaa gtgactcaca
    12241 tggtgatgcg ttatgcgccg gtgggtgtgt ttgcgctgat tgcggtgacg gtggctaact
    12301 ttggtttctc gtctctgtgg ccactggcga aactggtgct gctggtgcat ttcgctattc
    12361 tgttcttcgc gctggtcgtg ctgggaattg tggcgcgcct gtgcgggtta agcgtctgga
    12421 tcctgattcg tattctgaaa gatgagctga ttctggcgta ctccactgcc agttctgaaa
    12481 gcgtgctgcc gcgaattatt gagaagatgg aagcctacgg tgcaccggcg tcgatcacca
    12541 gtttcgtggt gccgaccggt tactctttta accttgatgg ttcgacgctg tatcaaagta
    12601 ttgccgctat cttcattgcg cagttgtatg gcattgacct gtccatctgg caggaaatca
    12661 ttctggtgct gacgctgatg gtgacctcga aagggattgc tggcgtgccg ggcgtgtcgt
    12721 ttgtggtgct gctggcaacg ctgggtagcg taggtattcc gctggaaggt ctggcattta
    12781 ttgccggtgt tgaccgtatc ctcgatatgg cgcgtactgc gctgaacgtg gtgggtaatg
    12841 cgctggcggt gctggtgatt gccaagtggg aacacaaatt tgaccgtaag aaagcgctgg
    12901 cttatgagcg tgaagtgctg ggcaaatttg ataaaactgc ggatcaataa ttgaagattg
    12961 ccggggatat ccacccggca atgtgtggat gcctgatgcg acgcttaacg cgtcttatca
    13021 ggcctacctt aaccgttagt taatttttca aactcttcac acccggtatc aaacccttcc
    13081 atacagctca gatttaacca gtgcagcgcc ttttgcttgt tcttctcgat aaaaccttct
    13141 tcaccattta agaacatcat tcccgcccag tactcggaat aaccggttcg ggaaatcgca
    13201 gagctgcgtt tgaaatacca ggttgccttg tcatcgtcgg ccttaatgcc cacgccgttg
    13261 gcgtaaatca aaccaagcag catttgggca tcgacggcag agtcgttctc cagatcttcc
    13321 gaagcgtttt ctaacagcga aatcgccttt ggataatccg gtttacccgc ttgcgtattt
    13381 accagaatat gcgccagcgt tacttcacct tctttactcc ccgcttgcgc cgctttctct
    13441 gcaagaactt ttgcttgtgg atagtccaga ctgactggat tggtgatttt gatctgcgcc
    13501 aacagcgcgc aggcatcagc atcaccgctg tcggcggctt tctgtgccca atattcggct
    13561 ttgctcaaat cgccggagct aaaccagctg tcggcaagaa aatattgtgc gcgtcggtcc
    13621 ccggcctctg ctgcctttaa atactggctg ccaggctcgg agtcgttcgc gtgggcaaag
    13681 aatgtcaaaa acaacatcaa tgcgataatt tttttcattt tgataatacg attgggtagg
    13741 gagtaaccag tataaagagt tcgaatggat aaaaaaacag cctccgaaag gaggctgtag
    13801 aaaggactgt attacgccag tgccgcttcg cgcaggcgag ttttcaactt gttgtactcg
    13861 tcaatcacgt actgctcggc ggcgcgctga tcggcgatcg gctcgacgcg cacggcgcag
    13921 tatttgtatt ccggcgtttt cgtaatcggg cttaagtttt cggtaaccag ctcgttacag
    13981 gcaccaatcc accactggta ggtcatgtaa atcgcccctt tgttcggacg atcgctgacc
    14041 tgcgcacggg tgataatttt gcctttacgc gagtgcaccc aaaccaatgc ctcatcttca
    14101 atacccagac gtttggcgtc ttcggtattg atttgtgcgt agccaggttc atcagccagc
    14161 gccgccagtg ccgcacagtt accggtcatc gaacggcaag agtagtgacc aacttcacgc
    14221 accgttgaca gtaccatcgg atattcgtcg gtgagtttgt cgattggcgc tacccagtcg
    14281 caggtgaaga actgcgccag accgttcggg gtatcaaact tctctttaaa caaataagaa
    14341 gtcccctgat cggcatctga agtatcgcga caaggccact gaatgaagcc cagttcgccc
    14401 attttctcgt aagtcgcacc gtagaaatcc gggcacagat gacgcaactc atcccagatc
    14461 tcctgggtgt tgttgtagtg catcggataa cccatacgag tggcaatttc actgatgatt
    14521 tgccagtccg ttttcagatc ccacttcggc tcaaccgcct tgaagaaacg ctggaagcca
    14581 cggtcagccg cagtaaacac gccttcatgc tcgccccacg acgttgacgg taaaatgaca
    14641 tccgccgccg acgcggtttt ggtcataaag atgtcctgaa cgatgaccag ttccagatct
    14701 tcaaaggctt tacgtactgc cgacagctcc gcgtcagttt gcagcggatc ttcgcccata
    14761 atgtacgcgg cacgcacttc gccatgcgct gcgcggtgcg gcagctcgct gatgcgatag
    14821 ccggtatgag caggcaggct ttccacgccc caggctttgg cgaatttctc gcggttagcc
    14881 ggatctttca cgtactggta tcccggatac gtatccggca gcgcgcccat atcgcaggca
    14941 ccctgcacgt tgttctgacc acgaaccggg tttacacccg catgcggctt accgaggtta
    15001 ccggtcagca tcgcgaggct ggtcagagaa cgcacggttt ccacgccctg gtagaactgg
    15061 gttacaccca tgccccacag gatggcggcg cttttcgcct gggcatacat ccgtgccgcc
    15121 tgacgaatct cactggcgct gacgccggtg atatcttcaa ccgactccgg cgtgtagcct
    15181 tcaacgattt tacgatactc ttcaaagcct tctgtacggg aagcgacgaa tgctttgtcg
    15241 tacagatttt cttcaataat gacatggccc atcgcattca acagcgcgat gttcgagccg
    15301 tttttcagtg caatgtgcat gtccgcaatg cgcgcggttt caattttgcg cggatcgcag
    15361 acgataattt tcgccccgtt acgtttagcg ttaattacgt gattcgccac gattgggtgg
    15421 gaatccgccg ggttgtaccc gaaaacgaac actaaatcgg tattatcaat ttcgttaata
    15481 gcattgctca ttgcgccatt accgaccgat tggtgcagac ctgcaaccga tgggccgtgt
    15541 cagacacgag cgcagcagtc aacgttattg gtaccaataa cggcgcgcgc aaatttttgc
    15601 attacatagt tggtttcgtt acccgtacca cgcgaggagc cggtcgtctg gatggcatcc
    15661 ggaccgtact tctctttgat ggcgctcagg cgctcggcaa cgtaattcag tgcctcatcc
    15721 caggaaacag gttcgagttt gccgccacgc tggcgacgga tcatgggggt tttcaggcgc
    15781 ggggtcagga tctgggtatc gttaatgaaa tcccagccat aataaccctt cagacacagg
    15841 gtaccctggt tggttttccc ctgcgctgcc tccgcccgga cgattttgcc gttatcgacg
    15901 accaggttga ttttgcaacc tgatgcgcaa taggggcaaa ccgtgacgac ttttttcatc
    15961 ggtctcgctc cagttaatca aatcacgcat acgcgctctc gactacagta tgcatctttt
    16021 atgccacatt ttatttgggg tcattccctg atattacggg cactatttat tcaaaactct
    16081 gacgaaaaac aggctgtcgt cagttttgac gtgacgaaac gaaataccgc gtgacagcca
    16141 tcacgcggca gacattttat tttttctcga cgacgggact tgcctgatac ccgccgccca
    16201 gcgatttcat caactgaatg ctttggatca cccggcggct gtccagcatc agtaatgaca
    16261 tctcctcggc aagcactggc aaccgggctt cggtggcctg taagcggctg gttaagccgc
    16321 gttgataggc ggcctcggca gcgcgctggg tgaagcgcgt tgcttccacg cgttcagcct
    16381 gcatctctcg ctcgtcgttg agcgtttgca gacgcgtgcc gttgacggca acgtcgcgca
    16441 ccgcattcag cactgactgg ttgtaacgtt caatcatcat gttgctggcg gcgcgagtgc
    16501 cttcgagatt ggcattcaac cgtccaccgt caaacagcgg caatttcaga cctgggataa
    16561 agttgaactg gcgactggtt tttttgaata aggtatccag gtggatagaa tccaggccga
    16621 aaaacgcttt gatatcaaag ctcggataga acagcgcccg cgcggaatcc acctgatcta
    16681 atgacgcctg aacataccag tgcatagctt gcagatccgg gcgtctggcg agcaactcat
    16741 aagagagtgt cgccggaata ccggtctgga cttgcggtaa tgccaccggt ttgatctccg
    16801 gcatatcgct ggctcccgcg ccaatcaatg cacgcagaga ttctcgcgtt tcggtgattt
    16861 gccctttaac ggcagcaatt tgtttatcga ccgccagaat ctgtgcccgc gcgccgtgga
    16921 aaggcacttg cgcttccaga ccgtgcgcca ctttactctg gtgcgctttc accgcgtaat
    16981 caatcacatc gcgtgtttgt tctaacagat cgagcatctg atagctggcc tgcatactgt
    17041 aataaagctg cgctacgccc gtggtcagcg atagctctac tgctgcggtt tctgccagcg
    17101 cggcattatg cgcgccaatg gcggcggcaa ccgctgagcg atgcacaccc cacaaatcaa
    17161 gatccagtcc ggcaaacaaa ccgactgtgg cttccgtata gtacggcccg tccataccca
    17221 gtgctggcgc atccatcgca taagggctta aaaagccgtt cgccgagaca cgttggcggt
    17281 tgagcatccc taacgctgcc acctgcaact gtgaaccagc atctaacaaa tcggcctggg
    17341 actgcgcttt ttcttcccgc agtttcgctt cggcgagggt gtgtgaacca cttagcgtcc
    17401 gttggatcag ggcatccaac tgcgggtcat tgagttgttt ccaccactgc gcctgcggcc
    17461 agccggagct ggcaagatga atatcatcgg ccagtttgat ttgttccggt ttgagctgtt
    17521 gatgaggtgc agaatcctta cgtaccaggg cacagccgga aatcagcgtc gtgctgccga
    17581 gaatgctgca caacagcaga cgtgaaagtt gacgattgat cattgcgtgg ctccttgtgc
    17641 ctgttccgtg gcgggcgtta atgccggggc tgtccagtct ggcaagcggg cgaaaagctg
    17701 gcagacgtgt tgctcctgtt ttaataaggt gggcaggatg gcctgcgagg cgggtgtttc
    17761 ttcaagcgtt atttgaggag aacggctgag tgcggttgcc agaccggcgg cgtatttctc
    17821 cagtgcgtcg gcaaactgac ctgctctgtc cggctgtagt gcgttatcca gcgcctgcgc
    17881 cgagttccag gttgcatccc aggcgtgaag aatatcgcgg ccctgacgaa taaccgtttg
    17941 cgaacgttca atcagtaaag cgcgttcttc gctgtccagt tgacgctcca acgccacgcg
    18001 ttggcacatc tcttcacagg cattaaacgc cgcatgcaga cctatacgaa tttgcaaata
    18061 agtgcgcaga gctgtgactt cctgctggcg tgggatccgc attactttac tcaacatacc
    18121 cagcgcgcca gccagttttt gcggcagtgt gcgcgcttca ctttcaggcc agacaaaggt
    18181 gtaaatcacc gcggacacca cggtaccaat gatgataccc agggcgcgat cgcgaatttc
    18241 caccaggtcg tacaccgggc caaaaacgtt ttcgagcgtg gcgagcgcga aggtgaccac
    18301 catctgtgtg ccgatataag aagagcgttc agagctggtg gcaatccatg cgcccaacag
    18361 aaaaatcggt gccagcacaa atagcaattc gacaatattg tccagccagg gcatgaccag
    18421 taacgtgaat aacagcgcca gaattgcacc gcagaaggca ccgccaaaac gcagcgccat
    18481 tttctgatac gacgaaccaa cattcgggtt agcgacgatc acgcaggtca gcatacaggt
    18541 gtgaatgcct tcccagtcca cgccgctgta gaaggtgtaa cagatcaaac aggcgagcag
    18601 cgtttttacc gcgtagcgca tatagtctgg attggtaaaa gcgtcggcga ccattgatgg
    18661 cggtttggtg gcgggcgttg gcggcgtatt cgggtccatc tgacccagtt gcaataacgt
    18721 ctggcagata ttctccaggt tacattcccg tgccgccatc gcttcacttt cagtgatccg
    18781 ccagtcgctt tgccagcact gaccttcggc aatggcatgc tgcagtttgt tgatttctga
    18841 ggctaatttt tgccgaaatt caataattgc ctgagaatca gcaaaagagg tgggatcgta
    18901 gcgattcagc gtcgagtaaa tgtaggttac cgttgccacg cagctttgcc accatgcgct
    18961 ctgagttcgc cagttggcat cgtccgcgag acaaaataca ttcagttttt gcagcgccag
    19021 cgcctctctt tcaatccgcg tttcgggtag cggtgtgagg ctgttcgtca ggtggctaat
    19081 ggcatcatca agccgatcat taagcgcctg atgcatctgc gtgatagcgc ggctgggaaa
    19141 ccacagcacg ccgattaacg tcatcaataa cgtcgggtag aggccgacaa cgatacacca
    19201 cagggttaag cgcacgacca cttccggata gtcgagcatg gcggggaagg tttgcccgta
    19261 aatggcgaca atggcgacag cgaaaaagac cagtcccagc cgatgggtgc gcatcaaaaa
    19321 catgcagccc atcaggatcg gcccggcgat gatcaatcgg atcaacggtt cgccgtatga
    19381 ccatttgtag atcaaaaaca ggctgccgat ctccagcacc gtggcaacca caaacaagat
    19441 cgccacaaat ttggtgtaaa acgcgttcga ctgaataccg taaaacagca ctgccagcga
    19501 taacgccaca aaagggatct caaaagtcat cgagatcaga atcaccagta ggcagcctac
    19561 ccagagttgc acggtctgcg gcacacgacc tggccgccgc tcgcttaact cttcatgaaa
    19621 gaacgccagc agcctgacca ccggtaatgg cagggagttg agcgcgctca ttattgcggc
    19681 tcaagattag cgactgccga agtgccgatg cggaacattt ccgggtcagg tttatcaacc
    19741 atgattttga ccggaaaacg ctgggcaacg cggacccagt taatagaacg agacactttc
    19801 ggcaggccgc ccagcaccag gccgccgtca tccggtagca cgccgtagcc aatcgaatcc
    19861 actttaccct cgaaggtttt gccgctgtcg ctcatcaggc gaatggttgc gggtgtacct
    19921 gagcgaatat ttttcagatc agtttcgcgg aagttggcga tcacgtacca gtgacgagtg
    19981 tcgattaggg tgaaaatagg gcgcatggca gaagcaaatt gcccgacgga ggttttgagg
    20041 gaaatgaccc ggccatcaaa cggcgcgcga acggtcgcca tttccagatg cagtttcgtc
    20101 aacgcaatat ccgcttccac tgccgcacgt tgcgccacca gcgcatccac gccgctgacg
    20161 gcgctggcgg ctgactgcgc ctgcaacaat ccggcattaa gatcggcttc tgcggcgcgc
    20221 tgcgcggttc tcgcgcggtc aacgtcttcc gctgagacga aaccttcttt cagtaatggc
    20281 tcggtgcggc gtaatgtatc actggcctgt ttcgcggcgg cacgggcttt ttctaccgtg
    20341 gcattaaccg agtcggcacc aaactgttgc gcgtcaacgc tacgctgggt gagcataatt
    20401 tgcttatcca gcgccgcgag ggaggcttcg gctttcgcca gattggcctc gtatgggcgc
    20461 gggtcgatgc gaaacagcaa atcgccctgt ttcaccgcct ggttgtcggt gaccgccagt
    20521 tctacaatgc gaccgctgac ttccggcacc acatcaatgg tatctgctga cgcgtaggcg
    20581 tcattagttg atggcgcact gtctacgcgc cagataacga aaacaagggc aaccagcgcc
    20641 aacgcaacca ctaacagagc agggaattta ctgcgaggag cttttttcgg cgtactttcc
    20701 atgagcgcgt ctcaaataga ttagaaaaat gccagccaga gcagcatggc gtagagagca
    20761 aaaagggcgg tataaataat tccgacaaat gccagattga tatttgcccg ctgaataatt
    20821 cgcctcgtga tgagcgtcaa aataaggctg gcaatggcgc agaaaaacca gctgggataa
    20881 taagcgccga tcaccgggat tgccggagat aatgagcatc ccgaaaccat gaagacggga
    20941 attatccagg ctgttttttt gagttgcatc gccatgcgag agagaactgt tggcatatta
    21001 aatacatcct gtctgctgcg aacaatatct gtagtgaata tcaatatcca ttgaaggcgt
    21061 cagtatggct attttctcga tgaataaaaa tagctttatt cagccaccgc attcataact
    21121 tata
//
LOCUS       DAOTRK010000081        20672 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.081, whole genome shotgun sequence.
ACCESSION   DAOTRK010000081 DAOTRK010000000
VERSION     DAOTRK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.081"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1229)
                     /gene="rho"
                     /locus_tag="QIE41_002839"
     CDS             complement(<1..1229)
                     /gene="rho"
                     /locus_tag="QIE41_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDS3531711.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTN"
     gene            complement(1314..1415)
                     /gene="rhoL"
                     /locus_tag="QIE41_002840"
     CDS             complement(1314..1415)
                     /gene="rhoL"
                     /locus_tag="QIE41_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDS3531712.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(1556..1885)
                     /gene="trxA"
                     /locus_tag="QIE41_002841"
     CDS             complement(1556..1885)
                     /gene="trxA"
                     /locus_tag="QIE41_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDS3531713.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            2016..3281
                     /gene="rhlB"
                     /locus_tag="QIE41_002842"
     CDS             2016..3281
                     /gene="rhlB"
                     /locus_tag="QIE41_002842"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDS3531714.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            3417..4901
                     /gene="gppA"
                     /locus_tag="QIE41_002843"
     CDS             3417..4901
                     /gene="gppA"
                     /locus_tag="QIE41_002843"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDS3531715.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(4948..6969)
                     /gene="rep"
                     /locus_tag="QIE41_002844"
     CDS             complement(4948..6969)
                     /gene="rep"
                     /locus_tag="QIE41_002844"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDS3531716.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            7187..7636
                     /locus_tag="QIE41_002845"
     CDS             7187..7636
                     /locus_tag="QIE41_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDS3531717.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            7788..8069
                     /gene="ppiC"
                     /locus_tag="QIE41_002846"
     CDS             7788..8069
                     /gene="ppiC"
                     /locus_tag="QIE41_002846"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDS3531718.1"
                     /translation="MAKTAAALHILVKEEKLALELLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(8115..9590)
                     /gene="ilvC"
                     /locus_tag="QIE41_002847"
     CDS             complement(8115..9590)
                     /gene="ilvC"
                     /locus_tag="QIE41_002847"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDS3531719.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            9740..10630
                     /gene="ilvY"
                     /locus_tag="QIE41_002848"
     CDS             9740..10630
                     /gene="ilvY"
                     /locus_tag="QIE41_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDS3531720.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(10627..12171)
                     /gene="ilvA"
                     /locus_tag="QIE41_002849"
     CDS             complement(10627..12171)
                     /gene="ilvA"
                     /locus_tag="QIE41_002849"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDS3531721.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(12174..14024)
                     /gene="ilvD"
                     /locus_tag="QIE41_002850"
     CDS             complement(12174..14024)
                     /gene="ilvD"
                     /locus_tag="QIE41_002850"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDS3531722.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDMIAIDIPNRGIQLQVSDAELV
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(14089..15018)
                     /gene="ilvE"
                     /locus_tag="QIE41_002851"
     CDS             complement(14089..15018)
                     /gene="ilvE"
                     /locus_tag="QIE41_002851"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDS3531723.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(15038..15301)
                     /gene="ilvM"
                     /locus_tag="QIE41_002852"
     CDS             complement(15038..15301)
                     /gene="ilvM"
                     /locus_tag="QIE41_002852"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDS3531724.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(15298..16944)
                     /locus_tag="QIE41_002853"
     CDS             complement(15298..16944)
                     /locus_tag="QIE41_002853"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDS3531725.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPVTCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQVHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(16947..16997)
                     /locus_tag="QIE41_002854"
     CDS             complement(16947..16997)
                     /locus_tag="QIE41_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531726.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(17084..17182)
                     /gene="ilvL"
                     /locus_tag="QIE41_002855"
     CDS             complement(17084..17182)
                     /gene="ilvL"
                     /locus_tag="QIE41_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDS3531727.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            17535..19055
                     /locus_tag="QIE41_002856"
     CDS             17535..19055
                     /locus_tag="QIE41_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDS3531728.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARKRQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(19080..19418)
                     /gene="maoP"
                     /locus_tag="QIE41_002857"
     CDS             complement(19080..19418)
                     /gene="maoP"
                     /locus_tag="QIE41_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDS3531729.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            19537..20376
                     /gene="hdfR"
                     /locus_tag="QIE41_002858"
     CDS             19537..20376
                     /gene="hdfR"
                     /locus_tag="QIE41_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDS3531730.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYKNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(20471..20546)
                     /locus_tag="QIE41_002859"
     tRNA            complement(20471..20546)
                     /locus_tag="QIE41_002859"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20511..20513),aa:Trp,seq:cca)
     gene            complement(20555..20631)
                     /locus_tag="QIE41_002860"
     tRNA            complement(20555..20631)
                     /locus_tag="QIE41_002860"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20595..20597),aa:Asp,seq:gtc)
BASE COUNT         4860 a         5488 c         5335 g         4989 t
ORIGIN      
        1 tcattggtct tggtcattgc cagtttatta atgaggaatt ccattgcatc gatttcgccc
       61 atcgggtgaa tgattttgcg caggatccac attttctgca gttcttcctg agtagtgagc
      121 agctcttctt tacgggtacc ggaacggttg tagtcgattg ccgggaagac gcgtttttca
      181 gcgatcttac gagagaggtg cagttccatg ttgcctgtac ctttaaactc ttcgtagata
      241 acttcgtcca tcttggaacc ggtatcgata agcgcggtag cgatgatggt caggctgccg
      301 ccctcttcca cgttacgcgc cgcaccgaag aagcgtttcg gacgatgcag ggcgttggca
      361 tccacaccac cggtcagcac tttacctgac gccggaacaa cggtgttgta agcgcgcgcc
      421 agacgagtga tggagtcgag caggatgata acgtctttct tgtgctcaac caggcgtttc
      481 gccttctcga tcaccatttc cgcaacctga acgtggcgag atgcgggctc atcaaaggtg
      541 gaagcaacaa cttcaccttt taccagacgc tgcatctcgg ttacttcttc cggacgttcg
      601 tcgatcagca gaaccatcaa cacacaatcc gggtggttgt aagcaatgct ctgagcaatg
      661 ttctgcagca gcatggtttt accggctttc ggcggtgcca caatcagacc acgctgacca
      721 cgaccgatag gtgatgccag atccagtacg cgagcagtta aatcttcagt agaaccgtta
      781 ccacgttcca tacgcagacg agagtttgcg tgcagcgggg ttaagttctc aaagaggatt
      841 ttgttgcggg cgttttcagg tttgtcgaag ttaacttcgt taactttcag cagcgcaaaa
      901 tagcgttcac cttctttcgg cgggcgaatc ttaccagaga tggtatcacc agtgcggagg
      961 ttgaaacggc ggatttggct aggggaaacg tagatgtcat caggaccggc gaggtaggag
     1021 ctgtctgcgg aacggagaaa accaaatcca tcctgcaata tctccagtac gccatcacca
     1081 aagatatctt cgccactctt tgcgtgctgc ttcaggatgg caaaaataat gtcctgctta
     1141 cgcatacgag ccaggttttc cagccccata ttttcgccga gagtgatcag ctcagaaacc
     1201 ggcgtattct ttaattcggt aagattcata gtggtgtgag ttcttaaact tggggtaaat
     1261 ctcgaactta atgttgtgaa tggtatggca ggatcatcca tgcctgttta cggtcatcga
     1321 ctcatgtctt ttcgctgcct ggtcacagga gagtacgcag aactgaaacg acaagacgga
     1381 ttgagtgacg agccagaaat ctgttcactt cgcatttaag ataaaacggg aagcgttgga
     1441 aattacaaga ttcaaactta ataaggtatg tttaatacga agtcaacact aagttagcat
     1501 gactcacgcc gggcgtccag tttttagcga cggggcaccc gaacatgaaa ttcccttacg
     1561 ccaggttagc gtcgaggaac tctttcaact gacctttaga cagtgcgccc actttggttg
     1621 ccgccacttc accgtttttg aacagcagca gagtcgggat accacggatg ccatatttcg
     1681 gcgcagtgcc agggttttga tcgatgttca gttttgcaac ggtcagtttg ccctgatatt
     1741 cgtcagcgat ttcatccaga atcggggcga tcattttgca cggaccgcac cactctgccc
     1801 agaaatcgac gaggatcgcc ccgtccgctt tgagtacatc cgtgtcaaaa ctgtcgtcag
     1861 tcaggtgaat aattttatcg ctcatatata actccacagg aataagcctg gcgtgttggt
     1921 ttcgttgttg gtgtaacatt aaccaactaa aggttgactt tatttcaccg gatacgcttt
     1981 cgtaaagcaa tagtaagctg atattctacc acactatgag caaaacacat ttaacagaac
     2041 agaagttttc cgacttcgcc ctgcatccga aggttgtaga agcccttgaa aaaaaagggt
     2101 ttcataactg tacgcccatt caggcactgg cccttccgct gacgctggcg ggtcgtgacg
     2161 tagccgggca ggcgcaaacc ggtaccggga aaacgatggc gtttcttacg tcaacgtttc
     2221 attatcttct ctctcatccc gcgattgccg atcgcaaggt gaatcaaccg cgtgccttaa
     2281 ttatggcacc gacgcgtgaa cttgccgtgc agatccacgc cgacgcggaa ccgctggcgg
     2341 aagctaccgg cctgaagctg ggtctggctt acggtggtga tggctacgac aaacagctga
     2401 aagtgctgga aagcggcgtt gacattctga ttggcaccac ggggcgttta attgactacg
     2461 ccaagcagaa ccacattaac ctcggtgcca ttcaggtagt ggtactggac gaagccgatc
     2521 gtatgtacga tctgggcttt attaaagata tccgctggct gttccgccgt atgccgcctg
     2581 caaaccagcg cctcaacatg ctgttctccg ccacgctttc gtaccgggta cgtgaactgg
     2641 cgttcgagca gatgaacaat gccgaatata ttgaagtgga accggaacag aaaacgggcc
     2701 accgtattaa agaagagctt ttctaccctt ctaacgaaga aaaaatgcgt ttgctgcaaa
     2761 cgctgatcga agaagagtgg ccagacagag cgattatttt cgccaacacc aaacaccgtt
     2821 gtgaagagat ctggggccac ctggcggcag atggtcatcg tgtcggtttg ttgacaggtg
     2881 atgtcgcgca gaaaaaacgt ctgcgtattc ttgatgaatt tacccgtggg gatctggata
     2941 ttctggttgc caccgacgtt gccgcgcgtg gcctgcatat cccggctgtg acgcacgtct
     3001 ttaactacga tttacccgat gactgtgaag attacgttca ccgtattggt cgtacaggtc
     3061 gtgcaggcgc aagtggtcat tccatcagcc tggcatgtga agagtatgca ttgaatttgc
     3121 ctgctattga gacctatatt ggtcactcaa ttccggtaag caaatacaat ccggacgcat
     3181 tgatgaccga tctgcctaaa ccgctgcgcc tcacgcgccc gcgtacaggc aatggtccgc
     3241 gtcgtactgg cgctccgcgt aatcgtcgtc gttcaggtta aataaaaaat gtcatactca
     3301 aaatcattca aactgtatca agatggcaag agaatgaacc ccgatgagct tactgtagta
     3361 agtgattcag gtgaaagaat gcagccaaca cagagacaga ttgaaggatg aagagtatgg
     3421 gttccacctc gtcgctgtat gcagccattg atctcggttc gaatagtttt catatgctgg
     3481 ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg aattaaacgc aaagtgcgtc
     3541 tggctgctgg cctgaacagc gaaaatgccc tgtctaatga agcaatggag cgcggttggc
     3601 aatgtttgcg cctgtttgct gaacgtctgc aagatatccc tccctcgcaa attcgcgttg
     3661 tcgctacggc gacattacga ctagccgtca atgcgggtga ttttatcgcc aaagcacagg
     3721 aaatccttgg ctgcccggta caagtcatca gcggtgaaga agaagcccgc ctgatttatc
     3781 agggggtggc ccacacaaca ggcggtgccg atcagcgcct ggtggtggat ataggcggtg
     3841 ccagtactga actggtaacc ggcacgggtg cacaaaccac ctcgttgttc agcctgtcga
     3901 tgggctgcgt cacctggctg gaacgctatt ttgccgatcg taatctggcg caggaaaatt
     3961 ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc ggttgccgat gaattacggt
     4021 atcacggctg gaaagtgtgc gttggcgctt ccggcaccgt gcaggcgtta caggaaatca
     4081 tgatggcaca ggggatggat gaacgcatta ccctggaaaa gttgcagcaa ctgaaacaac
     4141 gcgccattca ttgcggtcgg ctggaagagc tagagattga cgggctgacg ctggaacgtg
     4201 cgttagtgtt cccgagtggt ctggcgatcc tgatagccat ttttaccgaa ctgaatattc
     4261 agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct ggtctacggt atgttgcatc
     4321 ttaccgtcga gcaggatatt cgcagccgta cgctgcgtaa tattcagcgc cgctttatga
     4381 tcgatattga tcaggcacag cgcgtagcca aagttgcggc taacttcttc gatcaggtgg
     4441 aaaatgaatg gcatcttgaa gcaataagcc gcgatttgct catcagcgcc tgccagcttc
     4501 atgaaatcgg cctgagcgtt gacttcaaac aagcgccgca acacgctgct tatctggtac
     4561 gtaatctgga tcttcccggt tttacccccg cacagaaaaa attgctggcg acgctactgc
     4621 tcaaccagac gaatccggtc gatctctcat cgctgcatca gcaaaatgcc gtaccgccgc
     4681 gcgtcgcaga acaactctgc cgtttactcc gcctggccat catttttgcc agccgtcgtc
     4741 gtgacgatct cgtgccagag atgacattac aggctaacca tgaactgttg accttgacgc
     4801 ttccgcaagg ttggttaacc caacatccgc tgggtaaaga gattattgat caggaaagcc
     4861 agtggcagag ctatgtccac tggccgctgg aagtgcatta atgagtaagt gtcggatgcg
     4921 atgctgacgc atcttttccg gccttgatta tttccctcgt tttgccgcca tcatcgcttt
     4981 cagattcgcc agatggcttt gccctttctg catccgttct tcggcgctga ccactttacg
     5041 ctcctgttcc caaatcaaat catcctgcgg cagctccaat aagaatcggc ttggttccgg
     5101 gcgcaccagt tcgccgtact gacggcgttc tttacacagc gtaaaggtca attccttctg
     5161 ggcacgagta atgccgacat aggccagccg ccgctcctca tcgatattat cttcatcgat
     5221 gctgctctgg tgcggcaaaa aaccctcttc cataccgacc atgtagacat aaggaaactc
     5281 cagccctttc gacgcgtgca gagtcatcag ttgtacctga tccagctctt cctcgctctc
     5341 gccgcgctcc atcatgtcgc gcaaagtaaa gcgcgtcacc acctgggtga gcgtcatcgg
     5401 ctcatccagt tcgctgcctt ccagcatctc cgtcatccag ctaaacagtt ggttgacgtt
     5461 cttcatgcgc atttcggcag ctttcgggct gggcgatgtt tcgtacagcc aggattcata
     5521 atccatgccg tggatcagat cacgcaccgc ggcaatcggc tcccgctccg ccaggcgctg
     5581 gatttctgcc aaccagtgag tgaagcgggt caatgcttca taaccacgtc cgctaagcgt
     5641 ctgactcagg cccatatcaa agctggcggt aaacatgctt ttattgcgcg tcatcgccca
     5701 ctcacccagc tttttcagcg tcgccgggcc aatctctcgc ttcggcgtgt taacgatacg
     5761 cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc agataagcca gcaagtcctt
     5821 gatttcagga cgagagaaaa acgacgtacc accagatatt ttgtacggga tgcggttttg
     5881 catcaggaat ttttcaaata cccgcgactg atggttgccg cgataaagaa tggcataatc
     5941 tttgtactgc gttttattga cgaagtgatg cgcgatcagc tcgccggtga ctctctcggc
     6001 ttcatgttct tcgttattcg cgcttaaaac ttttagctcc gcgccatagc ccagttcgga
     6061 gaacagacgc ttttcaaaga cgtgcgggtt attggcaatg aggatgttcg ccgctttcag
     6121 aatacgtccg gaagagcggt agttctgctc aagcttaatc accttcagtg ccgggaaatc
     6181 ctgactcagc agcaccaggt tttgcggacg tgcaccgcgc caggagtaga tcgactggtc
     6241 gtcatcgcca actacggtaa agcgcgcgcg gctgcccacc agcagtttca ccagttcata
     6301 ctggctggtg ttggtgtcct gatactcatc caccagcagg tagcgaattt tgttctgcca
     6361 gcgctcgcgg acttcttcat tgcgttgcag cagtaacgtc ggcaataaaa tcagatcatc
     6421 gaaatcgaga acgttacagg ctttcaggtg tgcatcatac agcccataac aatgggcaaa
     6481 aatacggtcc cgctcgccaa tcgcactggc tgccgcctgg gacggtgttt tgagatcgtt
     6541 cttccagtta gagatggtcg aaatcagctg ttgcaggagg actttgtcat cttcaatcag
     6601 cccctcggtc agctctttaa gcaaagcaag ctgatcggta tcgtcaaaca acgagaagtt
     6661 tgctttcatc ccaagcgccg catactcgcg tttgatgata tccagcccca gtgtgtggaa
     6721 ggtggagatc atcagcccac gcgcctcttt gcgacccaac gtctgcccga cacgctcttt
     6781 catctcgcgc gccgccttat tggtaaaggt caccgccgcg atatgtcgcg cctgataacc
     6841 gcaaccgcgg atcagatggg cgattttatt ggtgatgaca cgagttttac cggaacccgc
     6901 gcctgccagc accaggcagg ggccggtaac gaattcgaca gcttgttgtt ggccggggtt
     6961 tagacgcata ggtgtattgc tcaatcttcg aacggggggg aggattgtag cagaatcgca
     7021 tttgaatgtg accgagtgaa aaccgctaac cggatggctg cggatgttta aatcagagag
     7081 ggaaggccga aaccggagtt catcatgaat ccattagact tagaaaatat cgggtcgtaa
     7141 cgttgtgtta ttactttttc agagtttact aaggagaggt ttactcgtgg cgattaattt
     7201 cagccccaag gttggtgaaa tactggaatg caactttggt aactatcctg ttagccagaa
     7261 tggacagttc agcacgacgt attacgatgg ccgcattcca cctgagatga taaaaaatag
     7321 gttggttgtc gtgctcaacg gaaaaattaa tggcaacgca tgtatcgtcg ttccattatc
     7381 aaccactcgc gaccatgaca agttaaatcg tggaatgcat gttgaaatag ccagtaatgt
     7441 tatcaatgac ctgcaattct ttgatcagca gatacgttgg gctaaagccg acctggtaca
     7501 acaagtcagt cgaaaccgat taaacagagc aaggacttat cgggggtatc ttaatcaatg
     7561 tttgccacat gagttggttg cagacataca gagggcagtc atcaagtcaa tcaatgccat
     7621 atcgctcata aattgacaat aaaatcacca tacccgataa tgactctgta gcccatagag
     7681 gccctaagtt ttctcccctt gagggacctg cgaagcccgg attttccggg cttttgccat
     7741 atatgtcccc cctccctgac tgacaatgtt taattcaagg cacgatcatg gcaaaaacag
     7801 cagcagcact gcatatcctt gtaaaagaag agaaactggc tctggaactt ctcgagcaga
     7861 ttaagaacgg tgccgatttc ggcaagctgg cgaagaaaca ctccatttgc ccgtcaggta
     7921 aacgcggtgg tgatttaggt gaattccgcc agggccagat ggttccggcg ttcgataaag
     7981 tggttttctc ttgtccggta ctggagccaa ccggtccgct gcatacccag ttcgggtatc
     8041 acatcattaa ggtgctttac cgtaactaac agcaaggcct tctccgggag aaggccttga
     8101 actctgaatc gcacttaacc tgcaacagca atacgtttca tatccgtcat atagccgcgc
     8161 agtttcttac ctacctgctc aatcgcatga ctgcgaatcg cttcgttcac atcacgcaac
     8221 tgcgcgttat ctaccgcacc ttccggaata gctttaccca ggtcgcccgg ttgcagctct
     8281 gccataaacg gtttcagcag cggcacacaa gcgtaagaga acagatagtt accgtattca
     8341 gcggtatcag aaataaccac gttcatttca tacagacgct tacgggcgat ggtgttggca
     8401 atcagcggca gttcgtgcag tgattcatag taagcagact cttcgatgat gccggaatca
     8461 accatggttt caaacgccag ctcaacgccc gcttttacca tcgcgatcat cagtacgcct
     8521 ttatcgaagt actcctgctc gccgattttg ccttcatact gcggcgcggt ttcaaacgcg
     8581 gttttgccgg tttcttcacg ccaggtcagc agtttcttat catcgttggc ccagtccgcc
     8641 atcatgccgg aggagaattc gccggagatg atatcgtcca tatgtttctg gaacagcggt
     8701 gccatgatct ctttcagctg ttcagacagc gcgtaagcac gcagtttcgc cgggttagag
     8761 agacggtcca tcatcagggt gatgccgccc tgtttcagcg cttcggtgat ggtttcccaa
     8821 ccgaactgaa tcagtttttc tgcgtatgcc ggatcggtgc cttcttccac cagcttgtcg
     8881 aagcacagca gcgagccagc ctgcaacata ccgcacagaa tggtttgctc gcccatcagg
     8941 tcagatttca cttccgcaac gaaggacgat tccagcacgc ccgcacggtg accaccggtt
     9001 gctgccgccc atgctttagc gatcgccata ccttcgcctt tcggatcgtt ttcagggtga
     9061 acggcaatca gcgtcggtac gccgaatcca cgtttgtact cttcacgtac ttcggtgcct
     9121 gggcatttcg gcgcaaccat gactacggtg atgtctttac ggatctgctc gcccacttcg
     9181 acgatgttga aaccgtgcga gtaacccagc gccgcgccgt ctttcatcag tggctgtacg
     9241 gtgcgcacta catcagagtg ctgcttgtcc ggcgtcaggt taaccaccag atccgcctgc
     9301 gggatcagtt cttcgtaagt accaacttta aaaccgtttt cggtcgcttt acgccaggat
     9361 gcgcgctttt cggcaatcgc ttctttacgc agagcgtagg agatatcgag accagaatca
     9421 cgcatgttca ggccctggtt cagaccctgt gcgccacagc cgacgatgac tactttttta
     9481 ccctgaaggt agctcgcgcc atcggcgaat tcatcgcgcc ccataaagcg acatttgccc
     9541 agctgtgcca gctgctggcg cagattcagt gtattgaagt agttagccat ggtgattcct
     9601 cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga gattgaattc actatatgac
     9661 aggaaattta ttgcggaaat tgatatattc acaacatcac attgcaattt ctgcaacgcc
     9721 aacattgagg gctgcgccat tggatttacg cgatctgaaa accttcctgc atctggcgga
     9781 aagccgccat tttggccgta gcgcgcgggc aatgcacgtt agcccgtcta cgctttcgcg
     9841 acaaatacag cgcctggaag aagatctcgg tcagccgctg tttgtgcgtg ataaccgcac
     9901 ggtaacgctg acggaagcgg gcgaagaact gcgcgttttc gcccagcaaa cgctgttgca
     9961 atatcagcaa ttgcgtcaca ccatcgatca gcaagggccg tcgctctctg gcgaattaca
    10021 tatcttctgc tcggtgaccg ctgcctacag ccatctgccg ccgattctgg atcgcttccg
    10081 cgcggaacac ccgtcagtgg agatcaaact cactaccggt gatgcggccg atgcgatgga
    10141 aaaggtggtc actggcgaag cggatctggc gattgcgggg aaaccggaaa ccttacccgg
    10201 cgcagtggcg ttttcgatgc tggagaatct ggcggtggtg ctgattgctc ccgcgctgcc
    10261 ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac tggtctacag tgccgtttat
    10321 catggccgat cagggaccgg tacgccgccg cattgaactg tggtttcggc gtaacaaaat
    10381 cagcaaccca atgatttacg ccacggttgg cgggcatgaa gcgatggtat cgatggtggc
    10441 gctcggctgt ggcgtggcgc tgttgccgga agtggtgctg gataacagcc cggaaccggt
    10501 gcgtaaccgc gtgatgattt tagagcgcag tgatgaaaaa acgccgtttg agctaggcgt
    10561 gtgtgcgcaa aaaaagcggc tgcatgagcc gctgattgag gcattctgga agattttgcc
    10621 gaaccactaa cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt cgtggcaatc
    10681 gtagcccagt tcattcagcc gggtttcaaa atccggttca tgatcgccaa gctcgaacgc
    10741 cgccagtacg cgcccgtagt cggtaccgtg gctgcgataa tggaacagcg agatgttcca
    10801 gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg ggaattcgaa
    10861 gctgtagagg cgttcctgca acggatgcga tggacgcccg ccaaccatat agcgcacatg
    10921 tagcttcgcc atttcgtcgt cggagagatc aaccacgctg tagccaccgt cgttgagcat
    10981 ctgcaaaatt tctttgcgct cttcgagacc gcggctcagg cgcacgccga caaaaatgca
    11041 ggcgtttttg gcatcagcaa agcggtagtt gaactcggtg accgagcgcc caccaagtaa
    11101 ctggcagaat ttgaggaaac tgcccttttc ttccggaatg gtcaccgcca gtaacgcttc
    11161 acgctgttcg cccagttcgc agcgttccga gacgtagcgc agaccgtgaa agttcacgtt
    11221 ggcaccggaa agaatatgcg ccagccgttc accgcgaatg ttgtgctggg cgatgtattt
    11281 tttcatcccc gccagcgcca gcgcgccgga aggttccgcc accgcgcgca catcttcgaa
    11341 taaatccttc atcgccgcac agatcgcatc actatcgacg gtgatgatgt cgtcgagata
    11401 ctcctggcat aaacggaagg tttcgtcgcc gatgcgtttt accgcgacac cttcggcgaa
    11461 cagtcccacg cggggcagat caacgggatg acccgcatcc agcgccgctt tcaggcaggc
    11521 ggaatcttcc gcttccacgg cgattacttt gatttgcggc atcagttgtt tgatcagcac
    11581 cgccacaccc gctgccagac cgccgccgcc gaccggtaca aatacgcggt cgagatgggc
    11641 gtcctgctgg agcagttcca gcgccagcgt gccttgcccg gcgatcacca tcggatggtc
    11701 gaacggcggt acccaggtga aaccctgctg ctgtgacagt tcgatcgctt tcgctttcgc
    11761 ttcatcgaaa tttgcgccgt gaagcagcac ttcgccgcca aagccgcgca ccgcatcaac
    11821 tttgatatcg gcggtggcgg ttggcatgac gatcagcgcc ttcacgccta accgtgcgga
    11881 agaaaacgcg acaccctgcg cgtggttacc tgcagaagcg gtaatcacgc cgtgcgcttt
    11941 ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg cgcaacttaa agctatgaac
    12001 tggttggcga tcttcgcgct tcaccagaat cacgttatcg agacgcgacg acagtttttc
    12061 cattttctgt agcggcgtga cctgcgccgc ttcgtagacc ggcgcgcgca gcaccgctct
    12121 taaatattcg gcaccttccg gggtaccgga caggggttgc gagtcagcca ttattaaccc
    12181 cccagtttcg atttatcgcg caccgcacct ttgtcggcgc tggttgccag gctggcgtag
    12241 gcacgcagcg caaaggagac ctgacgttcg cgatttttcg gcgtccaggc tttgtcgcca
    12301 cgagcttcct gcgcttcacg acgcgccacc agttcggcat cgcttacctg taactgaatg
    12361 ccacggttcg gaatgtcgat ggcaatcatg tcgccgtctt caatcagacc aatgctgccg
    12421 ccgcttgccg cttccggcga gacgtggccg atggaaagac cagaggtgcc gccggagaag
    12481 cgtccgtcgg tgatcagcgc gcaggcttta ccgagcccca ttgatttcag gaagctggtt
    12541 gggtagagca tttcctgcat gccggggccg cctttcgggc cttcgtaacg gatcaccacc
    12601 acatcgccag cgacaacttt gccgccgaga atcgcttcta ccgcgtcgtc ctggctttcg
    12661 tacactttcg ccggaccggt gaatttgagg atgctgtcat cgacgcccgc ggttttaacg
    12721 atgcagccgt tttctgcgaa attaccgtac agcaccgcaa ggccgccgtc tttgctgtag
    12781 gcgtgttcca gcgaacggat gcagccattt gcgcgatcgt catcgagaga atcccaacgg
    12841 caatcctgcg agaatgcctg ggtggtacga atgcccgccg ggccagcgcg gaacatattt
    12901 tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagcgtttg cggcaacgtc
    12961 aggccaagta catttttcac atcacggttc agtaatcccg cacgatccag ctcgccgaga
    13021 ataccgataa caccaccagc ccggtgaaca tcttccatat ggtatttttg ggtgctcggc
    13081 gcaactttac acagctgcgg aaccttgcgg gaaagcttat cgatatcact catggtgaag
    13141 tcgatttccg cttcctgcgc cgccgccagc aggtgaagta cggtgttggt agagccgccc
    13201 attgcgatat ccaacgtcat ggcgttttca aacgccgctt tgctagcaat attgcgcggc
    13261 agtgcacttt cgtcattttg ctcgtagtaa cgtttggtca attcaacaat gcgtttaccg
    13321 gcattgagga acagctgctt acgatcggcg tgagttgcca gcagcgagcc gttgcccggc
    13381 tgcgacaggc ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa catcccggag
    13441 caggagccgc aggtcggaca cgcggaacgt tcaacctgat cgctctggga gtcagaaact
    13501 ttcgggtctg cgccctggat catcgcatca accagatcga gcttgatgat ctgatcggac
    13561 agtttggttt tcccggcctc catcgggccg ccggaaacaa agatcaccgg aatattcagg
    13621 cgcagggaag ccatcagcat ccccggggtg attttgtcgc agttagagat gcagaccatg
    13681 gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat cagcgatcag ttcgcgagat
    13741 ggcagtgaat aaagcatccc cccgtggccc atggcaatcc catcatccac cgcaatggtg
    13801 ttgaactctt tggcaacgcc gccagccgct tcaatttgtt cggcgaccag tttaccaaga
    13861 tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac cgcgataatc
    13921 ggcttaccga aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc
    13981 atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta tttactccca
    14041 gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt attgattaac
    14101 ttgatctaac cagccccatt tatcttcggt ttcgccagtg aagaggccga agaaggcttg
    14161 ctgaatgcgt ttggtaaccg ggccgcaacg gccttcacca acctgaatac cgtctacact
    14221 gcgcactggc gtgatttctg ccgcagtacc ggacataaac acttcatccg ccaggtacag
    14281 tgattcgcgc gacagcacct gctcacgcac ttcaattccc agctctttcg ccagtttgat
    14341 gatggcgtca cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg tgaacagcac
    14401 accatctttc acttcaaaca ggttttcgcc tgcgccttca gagatataac cgttcacatc
    14461 cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca gcagggaaga
    14521 gaggtagtta ccaccggctt ttgccgccgt cgggatggtg tttggtgctg cgcggttcca
    14581 ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcacccagat aggcccccca
    14641 cgggaaagcg gcaataatca catcggttga gtatcccgct ggcgggttta cgcccatgcc
    14701 aacatcacca acgaagatca gcggacggat ataggcgctg gtgagattgt ttttgcggat
    14761 cacgtcacga caagcttcca tcagctcatc aatgctctgc gaaaccggga agcgatagat
    14821 tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa ccggtccttt
    14881 gtgcgagtcg tagcaacgga tgccttcaaa aaccgaggtg ccatagtgca gcgcgtgcga
    14941 catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc aaatgtaatc
    15001 agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt tgttgtgatg
    15061 tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt aactgactaa
    15121 acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata ttttgtgcat
    15181 cgctggcggc ggccatattc attgaacaga cgtggaaacc acgatgacgc accacgcgta
    15241 aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga tgttgcatca
    15301 tgataatttc tccaacattt ctgaattact ggcaccaggc ggcaccagcg gccagacgtt
    15361 ctcaagttcg tcgattgaga catgaagcag gtatggccca tcactgttca gcatggtgtc
    15421 gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc cgaaggcgct
    15481 ggctaacatg aggaaatcgg ggttatcagt aagggttgtt tcgctgtatc gttcctggaa
    15541 aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta agacgatttt
    15601 caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca tgaaagagcc
    15661 gtcaccggag atacagacga cggtatcgtt cggtcgcgcg acttgtgcgc caaccgccgc
    15721 aggtaaacca aaacccatgg tgcctaagcc gctggaggtg atgaaatttt ccgggcgagt
    15781 gtgggcgatg tgctgggcag cccacatctg gtgctgcccc acatctgtgg tcacgacgca
    15841 atccgcaggt tttcgatccg acagttgttt taacaacaac ggcgcgtaga tagcgtcacc
    15901 gggatggtcg taacgccagg tatgttcatc acgcagctgc gcgcagtatt gctgccagtc
    15961 attgatattt aacggctgct gtaatgctgg taaaagagca tttaaatcac cctgtaaagc
    16021 cacatgtacc tgacgcagct tgttcatttc tgccggatcg atatccatat ggataacgct
    16081 ggcgtgtggt gcgaaggtgt tcagtttgcc agtcacccgg tcatcaaaac gtgcacccac
    16141 ggcgatcagc agatcgcact cctgcactgc gaagttcgcc gctttggtgc cgtgcattcc
    16201 cagcatgccc agatagtacg gataatccgc ttcaactgcg cccagccctt tcagcgtgca
    16261 ggtgacaggc atttttgtgg tagcgagaaa ttcacgcaaa gccggaactg cctgcgccat
    16321 gccaacgcca ccgccaacgt acagcatcgg tttttgcgct tttgccagca tctggcgcgc
    16381 ttgctcgact tcggcatggg ggaaagtcac ttcgttttca acggtggtga accacggttc
    16441 cagatcgccg ctggctaact ggatatcttt tgggatatcg accagaaccg gaccaggacg
    16501 acctgagctg gcaacgtcga atgcttcagc catgatgcgc ggcaactctt ccagcgactg
    16561 caccagaaag ctgtgcttgg tacaggctaa tgacaatccc agtacatcca cttcctgaaa
    16621 tgcgtcagtg ccgataaacg gtgcagacac ctgaccggtg atggcaacga cagggatgga
    16681 atctaacagt gcgtccgcaa gcccggttat caggttggtt gcacctggac cagacgtggc
    16741 gatacatacg ccagttttgc cggtagcacg ggcataaccg atagccgcca ttgctgcacc
    16801 ttgctcgtgt cggcacagca agtgttccac gccaccgtca tacaatgcat cgtaaaccgg
    16861 cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg
    16921 taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga gaaacagaat
    16981 tttgtgctgt tattcattgt ctgctcctca gttatgtttt taaggtcaaa aaaaaccccc
    17041 ggacctttcg gtgcgggggt cttagttcgt taaggcttga tctctaagcc tttcctcgtc
    17101 caagtgcagc cccgcacggt gggataataa tcaccaccac gctaatcacg accaggctaa
    17161 tcactcgtag aagggctgtc attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa
    17221 agagttacca tagattttgc gaatagcaca agatattttt tggcctattt ttatgatgtc
    17281 tttaataatt tcaagagaaa ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaaatt
    17341 caatttcagt atgaatgttg ttcccttcct cgtagttcat cctttgcccg ctgcattttt
    17401 gcgagcatca ttccaccgcc agaacaactc aacttcactt acacaaataa accggaatca
    17461 gccactaaaa ttcacgaatt agcgggctac agcagcgata tcttatgcac ataatggtga
    17521 ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg cgcagccctg ggagtaaatg
    17581 cgcccccaat cactgttgag gtacatatca gtaaaggtct acccggctta acgatggtgg
    17641 gcttaccaga aacaacggta aaagaagccc gcgatcgcgt gcgcagcgcc attatcaata
    17701 gcggatatga atatccggcg aaaaaaatca ccatcaatct ggctccagcc gatctgccaa
    17761 aagaaggggg acgatatgat ttacctatcg ccattgcgtt gctggcggcc tcagaacagc
    17821 ttacagccaa taaattagat gaatatgaat tagtcggaga actggcgctt acaggcgctc
    17881 tgcgtggcgt tcccggcgca atctccagtg caactgaagc tattaagtcg ggcagaaaaa
    17941 ttatcgtcgc gaaagataac gaagatgaag tggggctaat taacggtgaa ggatgcctga
    18001 tagccgatca tctgcaggct gtctgtgcgt ttctggaggg taagcacgct ctcgaacgcc
    18061 cgaaaccaac tgatgcagta tcccgggcgc tacaacatga tctcagtgat gttgtcggtc
    18121 aggagcaagg aaagcgagga ctggaaatta ccgccgctgg cgggcacaac cttttactga
    18181 ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg tattaatggc ctgttgccag
    18241 atttaagcaa tgaagaggca ctggagagcg ctgcgatatt aagtctggta aatgctgaat
    18301 cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc tcatcacagt gcgtcgttaa
    18361 ctgcgatggt gggcggtggt gcaattccag ggccaggtga aatttcgctg gcgcataacg
    18421 gcgtgctttt tcttgatgag ctacctgaat ttgaacggcg tacactggat gccttgcgag
    18481 agccgattga atccgggcag atccatcttt cacgcacacg agcaaaaata acctatccag
    18541 cccgtttcca acttgttgcg gcgatgaatc ccagccctac cggacattat cagggaaacc
    18601 ataaccgctg cacaccagag caaacgctgc gttatctcaa ccgactctct ggtccctttc
    18661 tcgaccgctt cgatctctca ctggagatcc cattaccgcc acccggcatt ttgagtaaaa
    18721 cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg cgtaatggcc gccagaaagc
    18781 gccaatttaa gcggcagaat aaactgaacg cctggctgga taatccggaa atacgccaat
    18841 tctgcaagct tgagagcgaa gatgcgcagt ggttggaaga aacgctgatc catctggggt
    18901 tatcgattcg tgcctggcag cggttattga aagttgcacg aaccattgct gatattgatc
    18961 agtccgacat tatcacacgt cagcatttgc aggaggcagt tagctatcgt gccattgacc
    19021 gtttgctcat ccatctgcaa aaactactga cataaaaaaa gggcatttcg ccctttttat
    19081 taatcgtcag aatcggtgta gtcttcagca ccttcaacct gcggtttacc gccggaaagg
    19141 gtgtgaaaac gttttggacg cttgatacgc gtcatatact tggaccacac gcgttctgct
    19201 tctgtcactg gctcacgttc gccacggcat actgctacga agagtttctc ttcctcggta
    19261 accggctcgc gtttgccaag atccaactca ttgaaggcat aaccatgacg ctcaagcagt
    19321 tgtgcctctt tgatggtgaa gtcaccatga cgagagaatc cacgtggata atgtttattg
    19381 tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc tgcacgctcc taattctttg
    19441 accgagctag ttatggcgcg gagtattagt tacgcttgac agagtgtaaa acaaaacatt
    19501 taaatcataa cgacaaataa ttttgcggag agcactgtgg atacggaatt gttaaaaact
    19561 ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg ctgaatcgct ctatctgacc
    19621 caatcagccg tgagctttcg aatcagacaa ctggaaaatc aactgggtgt gaatcttttc
    19681 acccgccaca gaaacaatat ccgtttaacc gccgccggag aaaagcttct gccttatgca
    19741 gaaacgctca tgagcacctg gcaggccgcc cgtaaggagg tggcgcatac ctcacgacat
    19801 aacgagtttt ctatcggtgc cagcgcctca ttgtgggaat gtatgcttaa tcagtggctg
    19861 ggacgtttgt ataaaaatca ggatgcccat acaggcttac agttcgaagc gcgaattgcc
    19921 caacggcagt ctctggtaaa acagctgcat gaacgccagc ttgatcttct tattactact
    19981 gaagcgccca aaatggacga atttagcagt cagttgctcg ggtatttcac tttagcgctt
    20041 tataccagtg ccccttccaa actaaaggga gatcttaact atctgcgact ggaatggggg
    20101 ccagattttc aacagcatga ggcaggtttg atcggtgctg acgaagttcc cattctgaca
    20161 accagttctg ctgaactggc acagcaacag attgcgacgc ttaatggttg cacctggcta
    20221 cccgtcagtt gggcgcgtaa aaagggcggc ctgcataccg ttgtcgatag cacaacactt
    20281 tcacggccgc tttatgccat atggctgcaa aatagcgata aaaatgcgtt gattcgcgat
    20341 ctattgaaaa ttaacgtgct ggatgaagtg tattaatctg aatggctggc aaggatgccg
    20401 gtagaaggat ttacttcgga gaggttattt cagataaaaa aaatccttag ctttcgctaa
    20461 ggatgatttc tggcaggggc ggagagactc gaactcccaa cacccggttt tggagaccgg
    20521 tgctctacca attgaactac gcccctaatt agggtggcgg aacggacggg actcgaaccc
    20581 gcgaccccct gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc
    20641 ttttacaacc accggtttta tgaccggctt ac
//
LOCUS       DAOTRK010000082        20212 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.082, whole genome shotgun sequence.
ACCESSION   DAOTRK010000082 DAOTRK010000000
VERSION     DAOTRK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.082"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            38..1492
                     /locus_tag="QIE41_002861"
     CDS             38..1492
                     /locus_tag="QIE41_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDS3531731.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAENTGEQKVTG"
     gene            1553..2914
                     /gene="allB"
                     /locus_tag="QIE41_002862"
     CDS             1553..2914
                     /gene="allB"
                     /locus_tag="QIE41_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDS3531732.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKSEGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            2971..4272
                     /locus_tag="QIE41_002863"
     CDS             2971..4272
                     /locus_tag="QIE41_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDS3531733.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4294..5439
                     /gene="glxK"
                     /locus_tag="QIE41_002864"
     CDS             4294..5439
                     /gene="glxK"
                     /locus_tag="QIE41_002864"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDS3531734.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGEINLFNSARNIACAIKIGQGIKN"
     gene            complement(5569..6354)
                     /gene="allE"
                     /locus_tag="QIE41_002865"
     CDS             complement(5569..6354)
                     /gene="allE"
                     /locus_tag="QIE41_002865"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDS3531735.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFT
                     LSEGGYLYCPPGSLMTFVNAHAEDSQIFLYKRRYVPVEGHAPWLVSGNACELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6365..7600)
                     /gene="allC"
                     /locus_tag="QIE41_002866"
     CDS             complement(6365..7600)
                     /gene="allC"
                     /locus_tag="QIE41_002866"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDS3531736.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7622..8671)
                     /gene="allD"
                     /locus_tag="QIE41_002867"
     CDS             complement(7622..8671)
                     /gene="allD"
                     /locus_tag="QIE41_002867"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDS3531737.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            8988..10655
                     /locus_tag="QIE41_002868"
     CDS             8988..10655
                     /locus_tag="QIE41_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDS3531738.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLETTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARPDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALALQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10665..11924
                     /locus_tag="QIE41_002869"
     CDS             10665..11924
                     /locus_tag="QIE41_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDS3531739.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLNVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11935..12750
                     /locus_tag="QIE41_002870"
     CDS             11935..12750
                     /locus_tag="QIE41_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDS3531740.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDVLCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12747..13640
                     /locus_tag="QIE41_002871"
     CDS             12747..13640
                     /locus_tag="QIE41_002871"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDS3531741.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALTRLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYLPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13777..14844)
                     /gene="purK"
                     /locus_tag="QIE41_002872"
     CDS             complement(13777..14844)
                     /gene="purK"
                     /locus_tag="QIE41_002872"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDS3531742.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPIV
                     NSPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(14841..15350)
                     /gene="purE"
                     /locus_tag="QIE41_002873"
     CDS             complement(14841..15350)
                     /gene="purE"
                     /locus_tag="QIE41_002873"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDS3531743.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15468..16190)
                     /gene="lpxH"
                     /locus_tag="QIE41_002874"
     CDS             complement(15468..16190)
                     /gene="lpxH"
                     /locus_tag="QIE41_002874"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDS3531744.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16193..16687)
                     /gene="ppiB"
                     /locus_tag="QIE41_002875"
     CDS             complement(16193..16687)
                     /gene="ppiB"
                     /locus_tag="QIE41_002875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDS3531745.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            16861..18246
                     /gene="cysS"
                     /locus_tag="QIE41_002876"
     CDS             16861..18246
                     /gene="cysS"
                     /locus_tag="QIE41_002876"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDS3531746.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18282..18803)
                     /locus_tag="QIE41_002877"
     CDS             complement(18282..18803)
                     /locus_tag="QIE41_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDS3531747.1"
                     /translation="MPTIITHAAVPLCIALGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDAVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYSTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(18911..19123)
                     /gene="ybcJ"
                     /locus_tag="QIE41_002878"
     CDS             complement(18911..19123)
                     /gene="ybcJ"
                     /locus_tag="QIE41_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDS3531748.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQIVA"
     gene            complement(19125..19991)
                     /gene="folD"
                     /locus_tag="QIE41_002879"
     CDS             complement(19125..19991)
                     /gene="folD"
                     /locus_tag="QIE41_002879"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDS3531749.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         4598 a         5143 c         5571 g         4900 t
ORIGIN      
        1 aacaacgtta ttcgatacgt gaaattaaga gggatttatg gaacatcaga gaaaactatt
       61 ccagcaacgc ggctatagcg aagatctatt gccgaaaacg caaagccagc ggacctggaa
      121 aacatttaac tattttacct tatggatggg ttcggttcat aacgttccca attatgtgat
      181 ggtcggcggc ttttttattc tcggcttgtc tacctttagt attatgctgg caattattct
      241 cagcgccttt ttcattgccg cggtaatggt attaaacggc gctgcgggca gtaaatacgg
      301 cgtaccgttt gccatgatcc tgcgtgcttc ttacggcgta cgtggcgcac tgtttcccgg
      361 attattaaga ggcgggattg ctgccattat gtggtttggc ctgcaatgtt acgctggatc
      421 gctggcctgc ttgatcctga ttggcaaaat ctggccggga tttttaactc tcggtggtga
      481 ttttaccctg ttagggcttt ctctaccggg cttaatcact ttcttactct tctggctggt
      541 gaacgtcgga atcggtttcg gtggtggcaa agttttaaat aaattcactg ccattcttaa
      601 cccgtgcatc tatatcgttt tcggcggtat ggcgatttgg gcgatttcgc tggtcgggct
      661 cggtccaatc tttgactaca ttccgagcgg tattcagaaa gcagaaaaca gtggcttcct
      721 gttcctggtg gtgattaacg cggtagttgc ggtctgggcg gcaccggcgg tgagcgcatc
      781 cgactttacg caaaacgccc actcgtttcg tgagcaggcg ctggggcaaa cgctcggttt
      841 agttgtggcc tatattctgt ttgcggtcgc cggggtatgt attattgccg gagccagtat
      901 tcactacggc gctgacacct ggaacgtgct ggatattgtt cagcgttggg atagcctgtt
      961 tgcctcgttc tttgcggtac tggttattct gatgacgact atctctacta acgccaccgg
     1021 taatattatt ccggccggtt atcagattgc cgctattgca ccgacaaaac tgacctataa
     1081 aaacggcgta ctgattgcca gtattatcag cctgctgatc tgcccgtgga aattaatgga
     1141 aaatcaggac agtatttatc tgttcctcga tattatcggc ggaatgctgg gtccggtaat
     1201 tggtgtcatg atggcgcatt attttgtggt gatgcgtggg caaattaatc ttgatgaact
     1261 gtacaccgca cctggcgatt ataaatatta cgataacggt tttaacctca ccgcgttttc
     1321 agtaactctg gtggccgtta ttttatctct tggcggtaag tttattccct ttatggaacc
     1381 gttatctcgt gtttcatggt ttgtcggcgt catcgtcgcc tttgcggcct acgccttatt
     1441 aaagaaacgt actacagcag aaaacacagg agaacaaaaa gtcacaggtt aattaatccc
     1501 gatattgaac attgagtaaa aaaaccaatc tgtattttac aaggagtttg ttatgtcttt
     1561 tgatttaatc attaaaaacg gcactgttat tttagaaaac gaagctcgcg ttgtagatat
     1621 cgccgttaaa ggcggaaaaa ttgctgctat cggtcaggat ctgggcgatg caaaagaagt
     1681 tatggatgcg tctggtctgg tggtttcgcc aggcatggtt gatgcgcaca cccatatttc
     1741 tgaaccgggt cgtagccact gggaaggtta tgaaactggt actcgcgcag cggcaaaagg
     1801 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1861 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1921 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1981 cggcttcaaa tgcttcgtcg caacctgtgg cgatcgcggt atcgacaacg acttccgtga
     2041 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     2101 gctggtgcac tgcgaaaacg cgctgatctg tgatgcactg ggcgaagaag cgaaaagtga
     2161 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     2221 gattcgccgc gtactgtacc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     2281 cagcagcccg gaaggcgttg aagaagtgac tcgtgcacgt caggaaggtc aggacgttac
     2341 ttgtgaatcc tgcccgcatt acttcgtact ggataccgat cagttcgaag aaatcggtac
     2401 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     2461 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc cgccggaaat
     2521 gaaagctggc aacatcatga aagcatgggg tggtatcgcc ggtctgcaaa gctgcatgga
     2581 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     2641 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     2701 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     2761 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2821 gaaaaccatc ttacgtggtg atgtgattta cgacatcgaa cagggcttcc ctgttgcgcc
     2881 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2941 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtgag ccgcgaaagc
     3001 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     3061 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     3121 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     3181 atggaagggc ccggtggcct gtggtgggga accatcctta ctatcaccct tggtgaagca
     3241 tcgcgcggga caccgatcaa cgatatcgct accagcctgg cggtggggat tgctctctct
     3301 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     3361 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cgcagctgac cactatcttt
     3421 ttcaaaggta tgctcgggct gccgtttggc atagccgacc cgaattttaa aattcagtta
     3481 ccgccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tattttcctg
     3541 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     3601 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     3661 ctcggcagtg gcggtgcttt gtctccgggg attattctga cggcggtgat tacaggtctg
     3721 gtgaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3781 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggatttat gacactgata
     3841 actgtaccgc tggcggtaat tccattttca ccgtttgttt catccattgg tttattaacc
     3901 cagactggcg attacacgcg gcgttcgttt atttatggca gcgttatttg cctgctggtg
     3961 gcgctggttc cggcactcac acgactgttt tgcagtatcc ctttacccgt gagtagcgcg
     4021 gtcatgctgg tttcttatct gcctttactc ttttcggcgc tggtgtttag ccagcaaata
     4081 acctttaccg ctcgcaatat ttatcgactc gcattgccgt tatttgtcgg catattttta
     4141 atggcattac cgcctgtgta tctgcaagac cttccattaa cgctgcgtcc tctgctcagt
     4201 aacggcttat tggtcgggat cttactggct gttcttatgg ataaccttat tccgtgggaa
     4261 cgcatcgaat aatttgttga aaaaggattg ataatgaaga ttgtcattgc gccagactct
     4321 tttaaagaga gcttaagtgc agaaaaatgt tgtcaggcaa ttaaagccgg gttttcgacc
     4381 ctctttcccg atgcgaacta tatctgtttg ccgatagcgg atggcggcga agggacggtg
     4441 gatgcgatgg tcgccgcgac gggtggtaac atcgtgacgc ttgaagtctg cgggccgatg
     4501 ggcgaaaaag tgaatgcttt ttatggcctt accggcgacg ggaaaacagc ggtgattgag
     4561 atggcggcag caagtggcct gatgctggtc gcgcctgaaa agcgtaatcc gttgctggcc
     4621 tccagttttg gtacggggga gttaattcgt catgcgctgg ataacggcat tcgccatatt
     4681 attctcggca ttggcggcag tgcgacggtc gacggcggta tgggcatggc gcaggcgctt
     4741 ggtgtgcgtt tccttgatgc cgacggccag gtgctggcgg ctaacggcgg caatttagcg
     4801 cgcgtggcaa gcattgaaat ggatgaatgc gatccgcgtc tggcgaactg ccatattgaa
     4861 gtagcatgtg acgttgataa cccgctggta ggggcacgcg gcgcggcggc ggtgtttggc
     4921 ccgcaaaaag gggcaacgcc ggagatggtc gaagaacttg aacaggggct gcaaaattac
     4981 gcccgtgttt tacaacagca aactgaaatt aatgtctgcc agatggcggg cggcggcgct
     5041 gcgggcggta tgggtattgc ggcggcggta ttcctcaatg cggatattaa accgggcatt
     5101 gaaattgtgt tgaatgcggt caatcttgcg caggcagtgc agggcgcagc actggtgatc
     5161 accggggaag ggcgcatcga ctcgcaaacg gcaggcggta aagcgccgct gggtgtggcg
     5221 tcggtggcga agcagtttaa tgtaccggtg attgggattg ctggcgtatt gggtgatggc
     5281 gtggaagtgg tgcaccagta cggcattgat gcggtattca gcattttgcc acgtctggca
     5341 cctttagccg aagtgctcgc cagcggtgaa atcaatctct tcaacagcgc gcgaaatatt
     5401 gcctgcgcca ttaaaatagg tcagggaatt aaaaactgac tcttgccatt agagcggatg
     5461 cattttatcc cgcatccgtt ttttacctga atttaccgca aaaaaagcca cggtataaac
     5521 cgtggcaaaa tccaacatag ctaaaaataa tcaggcgagt ggtatgactt aaatctctac
     5581 gtcgcggttg caatcttttg agtaaatata actaaacgct tcaccacgtc ctacgccata
     5641 accggcctgt aaggaataag cgcccataaa gatgtaatcg ccttttttca ccgggatcca
     5701 gttattgtcg aggttataaa ccccctgacc ggaaagaata taggcaccgt gttcctggac
     5761 gtgagtttcg atataaccgt ggctggcacc tggcgcaaaa gagaggatat gcatgttcat
     5821 atcaaaacct aactctttgg gcagaaaatc cagcagaata acatcgtcca tgccttcata
     5881 atgaatgcgt tcgagttcgc aggcattgcc agaaaccagc cacggcgcat gaccttctac
     5941 cggaacatag cgacgcttat ataaaaagat ttggctgtct tcggcatggg cgttaacaaa
     6001 cgtcattaag gagcctggcg ggcaataaag atagccacct tcacttaagg taaatgtttt
     6061 cccttcggct ttggcagtga tatttccaga gatcacatac aggaacgttt caatgccttc
     6121 gccaccgaag ccctgttggt tgccgccgtt ttgatgcagt gtgaccagat aatcaacaaa
     6181 agaggccccc agctttggcg tggagaggat tgtcgcgtca caattttcaa agcccggaat
     6241 aatatttttt accagaccgt ccggggttag cagcgcgaaa ttaccgtgtt taacaatcgc
     6301 acggttagcc agtaaatcat cgcggtaacc ggtgacgtta tttaaatatc ccatttataa
     6361 ctccttattt ctgccaggca agttgataaa gcatgagtgc caacgttttg accccttcgg
     6421 caaggtcggt aatattggtg cgttccgccg ggttatggct gatcccgttg atgctgggga
     6481 taaaaatcat gcaggttggt acgcgaggcg cgaaaatttg cgcgtcgtgc ccggcaccac
     6541 tgtgcatcac ccggtaattc agtttttctc tttcacacaa ttctgtcagg gtggcgacca
     6601 ggtccttatt catcggcacg ggttcttcgt ccatccataa atcgatatca ataccaatgt
     6661 ccatttcatc gcaaatcgcc cgcatgtcgt tttctaactg ctgggtgaaa tcgcgcagca
     6721 cggcagcgtc ggtatgacga caatcaatgg tgaacgtggt tttacccggc accacattca
     6781 ccgtattcgg gcgcggctct actttgccaa aggtcagaac cagcggatcg cccatttttt
     6841 tcgctttttc gacagactga tggcaaatgc gactgaaagc gtaaaccgta tcacgacgat
     6901 aacccatcgg cgtggtgcct gcatggtttg attcgccgtt cagcgtcacc gtataacgac
     6961 gctgcccgac aattgcattc accacgccaa ttgactgccc attactttcc agcacacagc
     7021 cctgttcaat atgcagttca acaaaggctt taatatcctg acgcggagtt agtggggcgt
     7081 tcggcagagt aaatccgcaa gccttcatcg catcgacaaa actatttcct ttggcatcac
     7141 agatattccg cacgtcgtca ggattcgcca gcccaaagat atttttactg ccccagaaga
     7201 catacgggaa gcggctgcct tcttcttctg ccatcgccac cacttcgacc gtacgtaacg
     7261 gcgcgccgta ttgcgttttc aaccagtcaa ttgccagcca cgccgccagc gcgccgaatt
     7321 gcccgtcgag gttaccgccg ttaaccacgg tatcgatatg cgaaccgctc agaaccactt
     7381 cctgtggata ttcggtgcca ctcaggcgac cgtataaatt ccccacttca tcgaaacgtg
     7441 tttccagccc gcttgctgcc attctttttt taaattgctg ctgggtttcc agccattccg
     7501 gcgaataaag taaacgggtc atcccacccg ttgggtcagc gccaaaagag gaaagccagg
     7561 gcagcgtttc ttctatggct tgacggaaat gtgtaatcat aagaaagtcc tgtctcaata
     7621 attattgcgc aaagggattt ttcgtttcgt atgacgtgtt ataaagcgcg tctgaaatta
     7681 aatactggta aatatcatca acaatttcga tgccttcgac ggcggctttg cgttgtttaa
     7741 tatcctgatc ctgtccggga taataaacct ggttaaaacc gggcgcgggg gtaatggcat
     7801 ttaattcgcg catggtctgg ctaagatgtt gacggaataa ttcgctggag gagaaaaagt
     7861 tcgggttaat aacgatatgt aattgcccca aattacgccc ggcgtgtaaa tcgtcataca
     7921 tcgaactaac ctgtcgcccg aacggtaagc cgagtaagac gcctgagagg acgtcaatca
     7981 tcatcatcag gccataccct ttcggcccgg cggcggggag cagagcatgt accgcgaacg
     8041 gatcggttgt tggtgctccg tttttatcga ccgcccaggt atccgggata gacatattac
     8101 gcgagcgggc gtcgagcact tttccccatg cctgtacggt agtcgccata tcaaaggtaa
     8161 ggatctcgtc gccttctccc ggcgcggcaa aggccagtgg gttagtaccg tagtaaattt
     8221 ccgcgccgcc aaacggcacc accattggat cggactggca catcgaaatg ccaattaacc
     8281 ctgcgcgagc tgcctgctgt acaaaataag agattgcgcc gctgtgaccc atccggctga
     8341 taccgaccac cgcaacgcca ttttgctggg cggtttttat ggcatgttcc atacccattt
     8401 tcgccgcgac ctgtccggcg gcattgtcgg catgtaaaat tgccgaacac ggcccggttt
     8461 cttcaagacg aaattccggt tcacggttgg tgccgccttt tgaaatgcgt tctgcgtagt
     8521 attcaacacg caccgcgcca tgagagtgga tccctctggc atcggcgtaa accaatactt
     8581 cagccacggt tgcagcgtgc tcacgtttta acccagcctg gcagagttta ttctcgatta
     8641 gctggtggag tgtttcccga ctgattttca tctgtcttcc tttttaacga cggtgtgaag
     8701 catgactgca attaacatac agggaaaata tctggattat gtgatccaga caggcaaaaa
     8761 aatatagtta gaatttattt gataatccgc tcgcttttaa cctggttttt aaaacaacca
     8821 cgcttattaa aaaataacac gtaatagcct ggcggttatt tgaattcttt tgttaataat
     8881 ccctgtgtga tattcatcac cttattttcc cgttgtcatc gataccgtaa ttgccacatt
     8941 aacactgctc gtgcaattgc catgggtgca atttttaagg agttgttatg atccacgcct
     9001 ttattaaaaa agggtgtttt caggattcgg tcagtttaat gattatttca cgaaaactaa
     9061 gcgaatcaga aaatgttgat gatgtttctg taatgatggg tacgcccgcc aataaagcgt
     9121 tattagagac gacaggtttc tggcatgacg attttaataa cgccacgccg aacgatattt
     9181 gcgtggcaat tcgtagcgaa gcggcggatg cggggatcgc gcaggcgatt atgcaacagc
     9241 ttgaagaggc gctaaaacaa ctggcgcagg ggtcaggcag cagccaggcg ttgacgcagg
     9301 tgcgtcgctg ggacagtgcc tgtcagaaat tacccgacgc cagtctggcg ctgatttcag
     9361 tggctggcga gtatgcggcg gagctggcga atcaggcgct ggatcgcaac ctcaacgtga
     9421 tgatgttctc cgataacgtc acgctggaag atgaaatcca acttaaaacc cgcgcgcggg
     9481 aaaaaggctt gctggtgatg gggccggact gcggtacgtc gatgattgcc ggcacgccgc
     9541 tggcttttgc taacgtgatg ccggaaggca acattggcgt cattggcgct tccggtaccg
     9601 ggattcagga gctgtgctcg cagattgcgc tggcagggga gggaattacc cacgcgattg
     9661 gtcttggcgg gcgcgacctc agccgtgaag tgggcggcat cagtgcgctg acggcgctgg
     9721 aaatgctcag tgcagacgag aaaagcgaag tgctggcatt tgtttcaaaa ccacctgccg
     9781 aagcggtgcg tctgaaaatt gttaatgcca tgaaagcaac cggcaaaccg acggtggcgc
     9841 tgtttttagg ttataccccg gcggtggccc gcgacgagaa tgtctggttt gcctcctcgc
     9901 tggatgaggc cgcacgcctg gcttgcctgc tttcacgcgt tacggcgcga cgtaacgcga
     9961 taacgcctgc cagcagcgga tttatttgcg gtttgtatac cggcggtacg ctggctgccg
    10021 aagcggcggg attacttgcc ggacaccttg gcgtggaagc cgatgatgcc catcatcacg
    10081 gcatgatgct cgacgccgac ggacatcaga tcctcgacct gggcgatgat ttttacaccg
    10141 tcgggcgtcc ccatccgatg atcgacccga ccttacgcaa cctgttaatt gccgatctcg
    10201 gcgctaaacc gcaagtgcgc gtgttgctgc ttgatgtcgt gattggcttc ggtgcgactg
    10261 ccgatcctgc cgcctcgctg gtgagcgcct ggcaaaaagc ctgtgccgcg cgtccagaca
    10321 atcaaccact gtatgccatt gccacggtga caggcaccga acgtgacccg caatgccgct
    10381 cgcaacaaat cgccacgctg gaagatgcgg ggatcgcggt cgtgagttcg ctgccggaag
    10441 ccaccttgct ggcggcagcg ttaattcatc cgctctcgcc tgccacacag caacacacac
    10501 cgtcattact ggaaaacgtc gccgtgatta acatcggatt acgcagcttt gcgctggcgc
    10561 tacaaagcgc cagcaaaccg gttgtgcatt accaatggtc gccagtcgcc ggcggcaata
    10621 aaaaactggc tcgtttatta gaacgtttgc aataaggggt tcccatgttt acatcagtgg
    10681 cgcaagccaa tgctgcggtt atcgaacaaa ttcgtcgtgc tcgtccacac tggctggatg
    10741 tgcaaccggc ttcttcactt atcagcgaac taaacgaggg caaaacactg cttcacgccg
    10801 ggccgccaat gcgctggcag gagatgaccg gacccatgaa aggggcatgt gtgggcgcat
    10861 gtctgtttga aggctgggcg aaagatgaag cgcaggcgct ggcaatgctg gatcaggggg
    10921 aagtgaactt cattccttgt caccatgtga atgccgtcgg gccaatgggc ggcattactt
    10981 ctgccagtat gccgatgctg gtggtcgaga acgtgaccga cggcaaccgg gcgtactgca
    11041 acctcaacga aggtatcggc aaagtgatgc gttttggcgc ttacggcgaa gatgtcctga
    11101 ctcgccatcg ctggatgcgc gatgtgttaa tgccagtatt aagcgcggcg ctggggcgca
    11161 tggagcgcgg tatcgatctc acggcgatga tggcgcaggg cattaccatg ggcgatgagt
    11221 tccatcaacg caacattgct tcctctgcac tgttaatgcg tgcgctggcc ccacaaattg
    11281 ctcgcctcga tcatgataaa cagcacattg cggaagtgat ggatttcctc aacgtgaccg
    11341 atcagttctt cctcaacctc gcgatggcct actgcaaggc ggcgatggat gctggcgcga
    11401 tgatccgcgc aggcagcatc gttacggcta tgacccgcaa cggcaatatg ttcgggattc
    11461 gggtcagcgg gctgggtgaa cgctggttta ctgcgcctgt aaacactccg caaggtctgt
    11521 ttttcaccgg cttctcgcag gagcaggcaa acccggatat gggcgatagc gcgattaccg
    11581 aaacctttgg tatcggaggt gcggcaatga tcgcagcgcc tggcgtaacg cgctttgtcg
    11641 gtgcgggtgg catggaagcg gccagagcgg tatctgaaga gatggcggaa atttaccttg
    11701 aacgcaatat gcagttgcag atccccggct gggattttca gggcgcgtgc ctgggactgg
    11761 acattcgtcg cgtggtagaa accggcatta cgccactcat caataccggt atcgcccata
    11821 aagaggcggg gatcgggcag attggcgcag gcaccgtgcg ggcaccgctg gcctgctttg
    11881 aacaggcgct ggaagcactg gctgaaagca tggggatagg ttgaggaacg cgcaatgacg
    11941 atcatccatc ctctacttgc cagccgtagc gcaccgaatt atcgccagtc atggcggtta
    12001 gcgggcgtgt ggcgacgggc gattaacctg atgacggaaa gcggcgaact gttaacgctg
    12061 catcgtcagg gtagtggttt cggccccggc ggatgggtgc ttcgccgcgc gcaattcgat
    12121 gtgttatgcg gtggattatg cggcaatgaa cgaccacagg ttgtggcgca agggattcgc
    12181 ctcgggcgtt tcacggttaa acagccacag cgttattgtt tgctgcgtat tacgccgcct
    12241 gcgcatcctc aaccgcttgc cgttgcatgg atgcaacgcg cggaggaaac cgggcttttc
    12301 gggccactgg cattggcggc aagcgatccg ctgcctgctg aattacgcca gtttcgtcac
    12361 tgttttcagg ccgcgctcaa tggcgttaag accgactggc ggcactggct gggtaaaggc
    12421 cccggattaa cgccaagtca tgatgacacg ctgagcggaa tgctgctggc ggcctggtat
    12481 tatggcgctt tagatgcgcg ctccggtcgc ccgttttttg cctgttccga caatcttcaa
    12541 ctcgttacca ctgcggtgag cgtcagttat ttacgttatg ccgcgcaagg atatttcgcc
    12601 tcgccactcc tgcactttgt tcatgctctg agttgcccga aacgtaccgc agcggcgatt
    12661 gattcgctgc tggcgctggg gcacacgtca ggggcagata cgctgttggg gttctggctt
    12721 ggtcaacaat tattacaagg aaaaccatga aaacactggt tgtagctctt gggggcaacg
    12781 ccttactcca gcgcggtgag gcgctgacgg cggaaaatca atatcgcaat atcgccagtg
    12841 ctgtacccgc gttgacgcgc ctggcccgtt cttatcggct ggcgattgtt cacggcaacg
    12901 ggccacaggt ggggctgctg gcgttacaga atctggcgtg gaaagaggta gaaccgtatc
    12961 cgctggatgt gctggtggcg gaaagccagg ggatgattgg ctatatgctg gcgcagagtt
    13021 tgagcgcgca gccgcagatg ccgcctgtca cgacggtgct gacgcgcatt gaagtttcgc
    13081 ctgatgatcc ggcgtttttg cagccagaga agtttattgg tccggtttat ctgccagaag
    13141 aacaagaggc actggaagca gcttacggct ggcagatgaa acgtgatggt aaatacttgc
    13201 gccgggtggt ggcgtctccg caaccgcgta aaattctcga cagcgaagcc atcgagttgt
    13261 tgctcaaaga ggggcatgtg gtgatttgca gtggcggcgg cggtgtgcct gtgacggaag
    13321 atggggcagg gagtgaagca gtgattgata aagatctcgc cgctgcgttg ctcgccgagc
    13381 agattaatgc cgatgggctg gtgatcctca ccgatgctga tgcggtatat gaaaactggg
    13441 gaacgccaca gcaacgtgcc attcgccatg ccacaccgga tgagttagcg ccatttgcca
    13501 aagccgatgg ttcgatgggg cctaaggtga ctgcggtgag tggttatgtc agaagccgtg
    13561 gtaaacctgc gtggattggg gcgttatcgc ggattgaaga gacgctggcg ggcgaagcgg
    13621 ggacctgtat ttcgctgtag tgatgagagc atccggcaat cgtcgcacca tgcctgatgc
    13681 gacgcttgac gcgtcttatc aggcctacaa tgggtaccgg agcggtaagc cggataaggc
    13741 gttcacgccg aatccggcaa gaatatgagc accaggttaa ctgaacttac tctgcgccca
    13801 aatcacgccg ctggcatact ccggcggcag cagcgggatt aaggcttcca gcgtcgcggt
    13861 cagacgcgat gtgtcgctgt cggtcaaatt cagatgcccc actttacgcc ccggacggac
    13921 ttctttgtcg taccagtgca gatgcaccag cggcagtttc agccagtcat aattcagatc
    13981 gctaccaatc aggttgatca tcaccgacgg actattcact atcggttgcg gtaacggcag
    14041 atcggtaatc gcccgcagat gcagctcaaa ctggctgatg ctggcaccgt tttgcgtcca
    14101 gtgaccactg ttatgcacac gcggagccag ttcgttgatc agcagacctt gcggggtgac
    14161 aaagcactcc atcgccatca cgcccacata gcccagctcc tgcataatcg ccgacagcat
    14221 ctcttcggct tgcgcttgct gctgcgcatt ggcctgcggg aaagcgacgc tggtacgcaa
    14281 aataccgtcc tgatgcaggt tatgcgtcag cggataaaac acggtgctac catcaaagcc
    14341 gcgcgcgcca accagcgaca cttcgccaga gaagtttatg ccctgctcga caatacattc
    14401 gccgtagcat tcagccggta actgttcggt ttcatcggcg cgtaaacgcc attgaccgcg
    14461 tccgtcatag ccaccagtgc gacgcttaac aatcgccagc tcacctaaac gatcaaacac
    14521 cgcaggccac tcgctgcgat cggcaagtaa ctgccacggt gcggtcggta ggtgaagctt
    14581 gtcgaaaagc tgcttctgag tcagacggtc agcaataatc gggaatacat cgcggttcac
    14641 aaaggccgga tggcgcgcca gctcgcgggt taatgcggtt tccggccagc gttcgatctc
    14701 agcggtaatc acgctttgtt gaaaaggcac cgccgccggt tcagcgtcca gcccgactgg
    14761 ccagacagca atgcctaacg gttcgcctgc ctggcgcagc atacggccta actgcccgtt
    14821 accgaggacg caaacctgtt tcatgccgca cctcgcgggt ccgggttttc cagcacttcg
    14881 tcagtctggg ctttgcgcca gtcattcaga cgctggtgta gctctttatc atgagttgca
    14941 agaatttgtg ctgccagtaa cgccgcgttt gccgcgccag ctttaccaat cgccagcgta
    15001 cccaccggaa tgccgcgcgg catttgtacg atggagtaga ggctatcgac accgctcagt
    15061 gcggcgctct gtactggcac gcccagcact ggcaccagcg ttttggcggc aatcatgcct
    15121 ggcaggtgcg ctgcgccgcc tgcgcccgca ataatgacct gataaccgtt ttcttcggcg
    15181 ctttcggcga agctgaacag tttatcgggg gtgcggtgag cagaaaccac ttcaacgtgg
    15241 tgcgggacat tcaggatttc gaagatttcg gcggcgaact gcatggtagc ccagtcgctt
    15301 ttggacccca tcacgatggc gacacgcgcc ggattattgc gggaagacat gcgtcttaaa
    15361 actcctgtgg tgcacaactc tcggctttag agggcacaga gaatagcacg gaaagagagc
    15421 aaggaaaacg gttgcgtggc tgtgaaatca gcaaagttgc gggtttttta aaacgggaaa
    15481 tgaatcagct caacgtcatc cgccgtgact ttgaccattg aaccttccgt atgccaggca
    15541 cccaacacga cgcggaaagc aggttgctga ttggcgataa gttcatgtac tgccgggcga
    15601 tgggtatgcc cgtggatcag ccattgcacc tgatgttttt ccatcgcact gaccaccgcg
    15661 ttttggttaa cgtccatgat cgccagcgat ttactgctgt tggcttcttt gctgttcgcg
    15721 cgcattcgcg cggcaatgcg tttacgcaca aacaacggca gggcgaggaa tagcgtttgc
    15781 agccagggtt tgtggacttt ggcgcgaaaa gcctgataac ccgcgtcatc ggtgcacagc
    15841 gtgtcgccat gcataatcaa cacccggcga ccatacagtt cgagcacctt ttcttccggc
    15901 aataacgtca tgccgctttc acgggcaaag cgtttgccga gcagaaaatc acggttgcca
    15961 tgaatgaaat agcagggaac gccggaatcg gacaccgctt tgatcgctgc cgccatctgg
    16021 cgatggagtg ggttgggatc gtcgtcgcca atccacgctt caaacagatc gccaagaata
    16081 tacagcgcgt cggccttgcg ggcttccccc gctaaaaaac gcagaaaacc ggcggtgatc
    16141 gccggttctt ccgcgcagag atgaagatct gcaataaaga gtgtcgccac gattactcgc
    16201 taacggtcac gctttcaatg ataacgtctt cttttggcac gtcctggtgc ataccgctac
    16261 gaccggttgc aacacctttg attttgtcta ccacgtccat gccttcaacc acttcagcaa
    16321 acacgcagta accccaacct tgcaggcttt cgccagagaa gttcaggaag tcgttatcaa
    16381 ccacgttgat gaagaactgt gcagttgcag agtgcggtgc ctgagtacgg gccattgcca
    16441 gcgtaccacg ggtatttttc agaccgttgt tggcttcgtt tttgatcggg tctttggtgg
    16501 ttttttgctt catgcccggc tcaaaaccgc cgccctgaat cataaagccg ttgattacac
    16561 ggtggaaaat ggtgttgttg taaaaacctt cgcggcagta gtccaggaag tttttaactg
    16621 tttcaggtgc tttatcgtca aaagttttga tgacaatatc gccgtgattg gtgtggaaag
    16681 taaccatttt tgcatcctgt tccgtttgat tggtgcttca acccggttcg ggtcatatat
    16741 agggtggtgt tatagcataa ccgcacgatc ggatcatcac gcaatgtatg ctgattcgcg
    16801 cgggaaatat gggtattata cgcaactcaa ttacccacac atgtctaaac ggaatcttcg
    16861 atgctaaaaa tcttcaatac tctgacacgc caaaaagagg aatttaagcc tattcacgcc
    16921 ggggaagtcg gcatgtacgt gtgtggaatc accgtttacg atctctgtca tatcggtcac
    16981 gggcgtacct ttgttgcctt tgacgtggtt gcgcgctatc tgcgtttcct cggctataag
    17041 ctgaagtatg tgcgcaacat tactgatatc gacgacaaaa tcatcaaacg cgccaatgaa
    17101 aatggcgaaa gctttgtggc gctggtggat cgcatgatcg ccgaaatgca caaagatttt
    17161 gatgctttga acattctgcg cccggatatg gagccgcgcg cgacgcacca tatcgcagaa
    17221 attattgaac tcactgaaca actgatcgcc aaaggtcacg cttatgtggc ggacaacggc
    17281 gacgtgatgt tcgacgtccc gaccgatccg acttatggcg tgctgtcgcg tcaggatctc
    17341 gaccagctgc aggcaggcgc gcgcgttgac gtggtcgacg acaaacgcaa cccgatggat
    17401 ttcgttctgt ggaagatgtc gaaagagggc gaaccgagct ggccgtcgcc gtggggcgcg
    17461 ggccgtccag gctggcacat tgaatgttcg gcaatgaact gcaagcagct gggtaaccac
    17521 tttgatatcc acggcggcgg ttctgacctg atgttcccac accacgaaaa cgaaatcgct
    17581 cagtccacct gtgcccatga tggtcagtat gtgaactact ggatgcattc cggaatggtg
    17641 atggttgacc gcgagaagat gtccaaatcg ctgggtaact tctttaccgt gcgcgatgtg
    17701 ctgaaatact acgacgcgga aaccgtgcgt tatttcctga tgtcgggcca ctatcgcagc
    17761 cagctgaact acagcgaaga gaacctgaag caggcgcgtg cggcgctgga gcgtctctac
    17821 actgcgctgc gcggcacaga taaaaccgtt gcgcctgcgg gtggcgaagc gtttgaagcg
    17881 cgctttattg aagcgatgga cgacgatttc aacaccccgg aagcctattc cgtactgttt
    17941 gatatggcgc gtgaagtaaa ccgtctgaaa gcagaagata tggcagcggc aaacgcgatg
    18001 gcgtctcatc tgcgtaaact ttccgccgta ttgggcctgc tggagcaaga accggaagcg
    18061 ttcctgcaaa gcggcgcgca ggcagacgac agcgaagtgg ctgagattga agcgttaatt
    18121 caacagcgtc tggatgcccg taaagcgaaa gactgggcgg cggcagatgc ggcgcgtgac
    18181 cgtctgaatg agatggggat cgtgctggaa gatggcccgc aagggaccac ctggcgtcgt
    18241 aagtaattgc gctattgccg gatgcgagtt ttcgcatccg gttatcgcct gcgccaccac
    18301 aacattccca tcagtagcat ccccggcaac cacacccaca ttaattcaga aataatcacc
    18361 tgatgcccgt acggcgtgct gtaacgagac aatgcaaacg gcgcgacttt aatcacctgc
    18421 cagggagcga aaaagcgttc atctgaccac ggccatagcc agccaacgcc tttaccacca
    18481 gtggttaccg catccagcaa gctgtgcgat agcaacgaga cggttaaaaa cagccagcag
    18541 cgaatcagcc cagccctgaa ccatcggcgt ccaatcagca cacataacag cgggacaaca
    18601 aacgcaaaca ccagcgaatg ggtaaacccg cgatgaccaa aaacattgcc gtaagcaacg
    18661 ccaaatttaa acgacaagac gtcggcatcg ggcagcatcg ccaggatgat tccggcaaat
    18721 aacaggcgcg gagggatgac tttcgaaccc aaccctaaag caatgcaaag gggaacggct
    18781 gcgtgcgtaa taatggttgg catgatggtc gcttcggcaa aatgtcgatg ctatcagcat
    18841 ggatgaacgg ggcgtagagg gcaaaagtct gaaaagagaa ccggcctgtt gttacaggcc
    18901 gggaaaggga tcaggcaaca atctgtacgc tgtgaccggc aaaactcact gtctgcccgg
    18961 caacgatttt gcagcgtttg cgcgtttcaa ccgcaccgtc gactttcacc tggccttcgg
    19021 caatcgcgat tttcgcctgc gcgccgcttt cgctccagcc ttccagtttc agcaagtcgc
    19081 acagctcaac gtgcggatgt ttacctaaag aaaatgtcgc catgttactc accctgtgga
    19141 tcatgatatt caacgcacgc ctgtagcgtg ttttcaatca gcgtggcaac cgtcatcggg
    19201 ccaacgccgc cgggaacagg cgtaatgtat gaggcgcgtt tagccgcgtc ttcaaacacg
    19261 acgtcgccca caactttgcc attttccaga cggttgatgc cgacatcaat cacaattgcg
    19321 ccttctttga tccagtcacc gggaataaag cctggcttac cgacggcaac gatcaataga
    19381 tcggcatttt ctacgtggtg acgcagattt ttagtgaaac ggtgagtcac ggtggtggtg
    19441 caacctgcca gtagcagttc catgctcatc gggcggccaa cgatattcga tgcgccaatc
    19501 accacggcgt tgaggccgaa ggtatcaatg ttgtaacgct caagcagcgt gacaataccg
    19561 cgcggggtgc agggacgcag acgcggcgcg cgctggcaca ggcgaccgac gttataagga
    19621 tggaaaccgt ctacgtcttt gtccggatga atacgttcca gcactttgac gttatcaata
    19681 cccgccggta acggcagttg aaccagaatg ccatcgatgg tgttgtcggc attcagcgca
    19741 tcgataagct ccaacagctc cgcttcgctg gtggtttccg ggaggtcata agagcgggag
    19801 acgaacccga cttcttcaca agccttgcgt ttacttgcga cataaatttg cgatgcaggg
    19861 ttactaccca ccagcacaac ggccagtcct ggtgcccgca gtccggctgc aatacgcgcc
    19921 tgaacttttt gagcaacttc agagcgcacc tgctgcgcaa tcgttttacc gtcaataatc
    19981 tttgctgcca tcagagagag gattccatct gttacgtagg tcgaagggga tgcgcctatt
    20041 ttgtcagaag cggggcgcgc tgtcaggttt cgtttcagat ttatcgcgtg aagcgacctc
    20101 gtgcgaaggt gaggcgcact gtcgctgaga ttgaaagctt catttttcgt ccatgatggc
    20161 gttgtaaatc tggaactgac ttatttcctt gtcaaaggat taagataatt ta
//
LOCUS       DAOTRK010000083        20211 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.083, whole genome shotgun sequence.
ACCESSION   DAOTRK010000083 DAOTRK010000000
VERSION     DAOTRK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.083"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(51..1058)
                     /gene="yejK"
                     /locus_tag="QIE41_002880"
     CDS             complement(51..1058)
                     /gene="yejK"
                     /locus_tag="QIE41_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDS3531750.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            1240..1467
                     /locus_tag="QIE41_002881"
     CDS             1240..1467
                     /locus_tag="QIE41_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDS3531751.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            1487..3247
                     /gene="pbgA"
                     /locus_tag="QIE41_002882"
     CDS             1487..3247
                     /gene="pbgA"
                     /locus_tag="QIE41_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDS3531752.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAASNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            3322..3398
                     /locus_tag="QIE41_002883"
     tRNA            3322..3398
                     /locus_tag="QIE41_002883"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3356..3358,aa:Pro,seq:ggg)
     gene            complement(3500..6091)
                     /gene="yejO"
                     /locus_tag="QIE41_002884"
     CDS             complement(3500..6091)
                     /gene="yejO"
                     /locus_tag="QIE41_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HDS3531753.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANEPTIKGGRLIIEKDGITVFAAIEKGGLLEVKEGG
                     LAFAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKDGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELYTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMSADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWSF"
     gene            6411..7058
                     /gene="narP"
                     /locus_tag="QIE41_002885"
     CDS             6411..7058
                     /gene="narP"
                     /locus_tag="QIE41_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDS3531754.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(7094..8146)
                     /gene="ccmH"
                     /locus_tag="QIE41_002886"
     CDS             complement(7094..8146)
                     /gene="ccmH"
                     /locus_tag="QIE41_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDS3531755.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(8143..8700)
                     /gene="dsbE"
                     /locus_tag="QIE41_002887"
     CDS             complement(8143..8700)
                     /gene="dsbE"
                     /locus_tag="QIE41_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDS3531756.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(8697..10640)
                     /gene="ccmF"
                     /locus_tag="QIE41_002888"
     CDS             complement(8697..10640)
                     /gene="ccmF"
                     /locus_tag="QIE41_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDS3531757.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAV"
     gene            complement(10637..11116)
                     /gene="ccmE"
                     /locus_tag="QIE41_002889"
     CDS             complement(10637..11116)
                     /gene="ccmE"
                     /locus_tag="QIE41_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDS3531758.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(11113..11322)
                     /gene="ccmD"
                     /locus_tag="QIE41_002890"
     CDS             complement(11113..11322)
                     /gene="ccmD"
                     /locus_tag="QIE41_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDS3531759.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVAMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(11319..12056)
                     /gene="ccmC"
                     /locus_tag="QIE41_002891"
     CDS             complement(11319..12056)
                     /gene="ccmC"
                     /locus_tag="QIE41_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDS3531760.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(12098..12760)
                     /gene="ccmB"
                     /locus_tag="QIE41_002892"
     CDS             complement(12098..12760)
                     /gene="ccmB"
                     /locus_tag="QIE41_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDS3531761.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGVIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(12757..13374)
                     /gene="ccmA"
                     /locus_tag="QIE41_002893"
     CDS             complement(12757..13374)
                     /gene="ccmA"
                     /locus_tag="QIE41_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDS3531762.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(13393..13995)
                     /gene="napC"
                     /locus_tag="QIE41_002894"
     CDS             complement(13393..13995)
                     /gene="napC"
                     /locus_tag="QIE41_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDS3531763.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(14005..14454)
                     /gene="napB"
                     /locus_tag="QIE41_002895"
     CDS             complement(14005..14454)
                     /gene="napB"
                     /locus_tag="QIE41_002895"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDS3531764.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(14451..15314)
                     /gene="napH"
                     /locus_tag="QIE41_002896"
     CDS             complement(14451..15314)
                     /gene="napH"
                     /locus_tag="QIE41_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDS3531765.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(15301..15996)
                     /gene="napG"
                     /locus_tag="QIE41_002897"
     CDS             complement(15301..15996)
                     /gene="napG"
                     /locus_tag="QIE41_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDS3531766.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(16003..18489)
                     /gene="napA"
                     /locus_tag="QIE41_002898"
     CDS             complement(16003..18489)
                     /gene="napA"
                     /locus_tag="QIE41_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDS3531767.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISKPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(18486..18749)
                     /gene="napD"
                     /locus_tag="QIE41_002899"
     CDS             complement(18486..18749)
                     /gene="napD"
                     /locus_tag="QIE41_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDS3531768.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(18739..19233)
                     /gene="napF"
                     /locus_tag="QIE41_002900"
     CDS             complement(18739..19233)
                     /gene="napF"
                     /locus_tag="QIE41_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDS3531769.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQTVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            19639..20127
                     /gene="eco"
                     /locus_tag="QIE41_002901"
     CDS             19639..20127
                     /gene="eco"
                     /locus_tag="QIE41_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDS3531770.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRIWKAEEKIDNAV
                     VR"
BASE COUNT         4917 a         5772 c         4946 g         4576 t
ORIGIN      
        1 ggccagataa ggcgttcacg ccgcatctgg cattcagaac acaaaaccga ttaattgccg
       61 ccagacgtcc ggcgctgcaa ttgatcgcgc aaattcggcg gcgtgccttt aatggtcagg
      121 gtatcggttg ctggatccca gaagatgcgt tcacctaaca gcatcgcatc aaagttaata
      181 gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc
      241 gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg
      301 ctgacgcctg ccagctcttt cgacagcgat tccagttcaa tctcttcgcc tgcctgcagc
      361 tgctcattac agtagctata aacctgctgg cgcacgttct gtcgctctgc tttatccagt
      421 tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc cacggttttg cgctttcgcg
      481 tttaaccctt cgctggcccc gaggaaatcc ataaagaaat ccgccacttt gcgccctact
      541 cgccctttca ggaaagtgag atagcgggtc gactctggat tggtttccca ctcggttaaa
      601 tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgggttgg gttgatgtcg
      661 agattttcgt taacacgcat actgctcagg ttgctcagaa ctgccaccag cagatactcc
      721 accgccagat agcgatagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc
      781 gctaattcgt cacgcaggcg accggttgcc gcgcggctaa atgccagaaa gtcctcttcg
      841 ccctgacgct gtaagcgcag tgtctgcgcc agttcactct cttcgctaaa caagccgtaa
      901 gctttatttt tggcgctata gacccgatgc agttctgcca ccatctcaac gacggtttct
      961 gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata
     1021 agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg
     1081 ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc attttacaga
     1141 aggcggcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg
     1201 taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac
     1261 agtgatgaac aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag
     1321 gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc
     1381 agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc
     1441 tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg
     1501 tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt
     1561 caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac
     1621 aacgcttgct ggtcgcattt attcctacgt aagcattatc ggccatttca gcttcctggt
     1681 gttcgccacc tacttgctga tcctcttccc gctgaccttt atcgtcggct cccagaggct
     1741 gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg acgctattac tgatcgatag
     1801 cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcagc tggttatcaa
     1861 ccctgacgaa aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg tgccggttat
     1921 tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgaa gcctgacgcg
     1981 tcgtcgacgc ttcgcgcgcc cgctggccgc attcttattt atcgccttta tcgcctcgca
     2041 tgtggtttat atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa
     2101 cctaccgctt tcgtacccga tgacggcgcg acgttttctt gagaagcatg gtctgcttga
     2161 tgcgcaggag tatcaacgcc gtctcattga gcaaggtaat ccagacgccg tttccgttca
     2221 gtatccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg tgctgttgat
     2281 tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt
     2341 tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc ggcaacacta cagacaacgg
     2401 catctttggc ctgttctatg gcatctcgcc gagctatatg gacggtattc tgtcgacccg
     2461 tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg ggttattctc
     2521 atcagatggc tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc
     2581 gagcgtacgc acccaatccg acgagcagac cgccacgcag tggatcaact ggctgggccg
     2641 ctacgcacaa gaagataacc gctggttctc gtgggtttct ttcaatggca ctaacattga
     2701 cgacagcaat cagcaggcat ttgcacggaa atatagccgg gcggcaagca atgtcgatga
     2761 ccagatcaac cgcgtgctca atgcactacg cgattctggc aaactggaca atacggtggt
     2821 gattatcact gccggtcggg gtattccgct gagcgaagag gaagaaacct ttgactggtc
     2881 ccacggtcat ctgcaggtac cattagtgat tcactggcca ggtacgccgg cgcagcgtat
     2941 taatgctctg actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctacatgt
     3001 cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgtca
     3061 ttactgggtt accgccgcgg ataacgatac gctggcaatt accaccccga aaaagacgct
     3121 ggtgctgaac aataacggta aataccgtac ttacaactta cgtggtgaaa gagtgaaaga
     3181 tgaaaaacca cagttaagtt tgttattgca agtactgaca gacgagaagc gttttatcgc
     3241 taactgatta attataaatc agttagcgaa atatcttact tgcaatcggt gtggaaaacg
     3301 gtagtattag cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt
     3361 gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaaa aaaaccaacc
     3421 tttacggttg gtttttttat atctgcattt aattcgataa acagaccgcg acacatcacg
     3481 gcctgtttat tttctgttat cagaacgacc agaccacacc cgcctgagta ttccacggtg
     3541 attctacccc tgcgccattg ccatagctga cagacaaatg accgcttaac gtcggggtaa
     3601 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
     3661 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgt acggcggctt
     3721 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
     3781 ccgcttcagc ccgtaatatg cgggtatttc ccacatccgc gctcatgccg tttgataacg
     3841 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
     3901 atccgtcaac ccaacggaac ccgctctcga catgagcacc cgcgccgtta ctattgtaat
     3961 cgccaaacgc tgttgctcca ttactcatct tgccatggat ggtgttggca aaacggtcaa
     4021 ctttcaccac tccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
     4081 gggtgtagct atcgatatta cctttgccgc cgcgatcaaa accaatatca gaatgagagt
     4141 aaccaaagaa caagccgcga attgtgctgc tttcttcacg ggagaaacgg ctatcgatac
     4201 cgagcgtcag gcccgtcaat gtttgctcaa agccagctcc cgcatcagtg gtcacgttgt
     4261 tgcgggtgtt aattgccgaa ctccacatcg ccgtatcgta actaacgcct tttacgctac
     4321 caagacgctc acgcacggtg tacagttctg catcaaatac cagcggttgt gctgctgcca
     4381 tgttcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcc agactccagc
     4441 tatggttgcc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
     4501 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatcac
     4561 ctgctgccgg gctggcccct gtgtccgtca caaatatttt gaaatcacct gttgcctgac
     4621 cggtcacgtt gagctgatcg ctctgatgat tagccatatc agtacgcata taaaaattgc
     4681 cgtttcccga tagggtattg gtggtcaacg tagtgaaatt ttccgcactt gccgtcacag
     4741 atgaacgagt cactggctca gccatcataa tcacatttgc gccagacatc gcaaggttgg
     4801 ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc
     4861 tattttcgag cgaaacatcc gctttgctgg tatccacggc accatgcgtt ctaaaagaag
     4921 cgccctcaga aaccactacc ttacccacca acatagcggg tgcataattt atttgcggct
     4981 tcatgacctc aagaatgcca tcattccctc tgactgaaac gttaaccatt gtgccagccc
     5041 agacattagc gcggccattg ttgataacca tattttcaga gatggcttta gaggaatacg
     5101 ttatacttcc attctgatct gatcgaccga gatcatatac cgcatttgcc tgcactctcg
     5161 taccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
     5221 cctgcgtgtc ctccataaca ataagcccgg aaccatcatc cagctcataa ttttttgaca
     5281 caccatcttt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
     5341 ttgagacaat taattttccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
     5401 taacctccag taagccgcca ctatccacag tggtattata agcgaagtca ttttcttcaa
     5461 ctcgcaaact tccgccgttt tccaacagca tattattagc aataccatcc ttgatatcga
     5521 actgaccgag acggtttgtt ccgaatacct ccatggcccg ggtggttgtt ttaatcgcac
     5581 cgcctgcttt ctgatctacc gcaaatgcta atcctccctc tttaacctcc agtaatcctc
     5641 ccttttcgat agcggcaaag acggtaatcc catctttttc aataattaag cgcccgcctt
     5701 taatcgtagg ttcattcgct gcgccataaa cctgtagcac accattattg agggtagtct
     5761 ggtttgccgc accacccatt tgaacaattt gataaccatc atttaatact gagtattctg
     5821 cggtgccatt catttcaatg tactgatacc cgccgttaat ttcagtatta ctacttatac
     5881 caaattcttt aatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
     5941 aaaccagctg gatatctttt tccaggacaa caccatcaac tgtctcacca tactcaactg
     6001 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
     6061 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgct
     6121 gttaaaagca ttgggtagag aaaataccca tagctccata cccggagtca gtttttaaaa
     6181 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
     6241 agctgatgca aaattccgtc tttataatga aagtgatgcc aaagcgaacg acaaggttgt
     6301 agttttcgct acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
     6361 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
     6421 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
     6481 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgac ggcgcgagcg
     6541 caatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
     6601 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
     6661 ttatcctgac cgtatcagat gcctccagcg atgtctttgc gctgatagac gcaggcgcag
     6721 acggttatct gttgaaagac agcgacccgg aagtgttgct ggaagcgatt cgtgccggag
     6781 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacgtgaa cgtgaaatgt
     6841 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
     6901 agctggcaca ggggctgtca aataaacaga ttgcctcggt gttgaatatt tccgagcaga
     6961 cagtaaaagt acacattcgc aatctgctgc gtaaactcaa tgtccgctca cgcgtggcgg
     7021 ccaccattct gttcctgcaa caacgcgggg cacaataaaa aatagcccga tggcatcacc
     7081 atcgggtctc tttttattta ctctcctgcg gcgacaaatg ttgcatcgcc tgcgcgatac
     7141 tacgttcaat caccgcacgg cgagtatcgt tggcaggtaa gagtttcaac atcatctccc
     7201 acgcggcaac ggcttcgcca aatcgctgct gctcaaaggc gttaaacgca tacatgctta
     7261 acacacgaat attgctatgg tccgttctca ccaactggcg cagcagttcg ccgccgaggc
     7321 ggttgtcgtt gggatcagat gaacgtgtca acgcttcagc gtatcccagt gcagcatcgc
     7381 tgttcttcgg atcgaggcga tacgcagtag cgtatgcatc ggtggcgata ctggcgttac
     7441 ccagcgccat gccaacgcgg cccaacataa tccagccttc tatatctccc ggattttttt
     7501 gcagttgagt acgcatcccc agcgcaaggc gcgacatctc ttcttcgttg agcggatcgg
     7561 ctttcggatc cagcgccctg tccagcaacg ccggagcctg tgccgtggcc tgctgccaga
     7621 ttttcacctg ctgataattg ccagtctggt agtagctgac gccagccaca attaacgcca
     7681 ccacaatacc cggcagataa acaatatatc cggcacgctt accttccggc acgctttgtt
     7741 caggaaacgc gtccggcacc acgcgtaccc gacgccgcga acgggcgtaa atgacccaac
     7801 cgccaatgcc aatagccact accggaagca cccacagcag cacggtcagc ggcgttaacg
     7861 gcggatcgta agtgacaaag ttgccgtaac gcgccaccat ataatcgaca atctcttttt
     7921 tacttttacc ttcctgcatc agttcataca ctttctggcg caggtcggtg gcaatcatcg
     7981 agttggaatc ggcaatgctg ttgttctggc atttcgggca gcgtagttct tcagtgagtt
     8041 gccggaactg ctgctcctgc gcttcatcct taaactgcaa cacgtcgatg gtcgccagcg
     8101 ctgagccgga gatcatcagc atcagcacgc ccaataaaaa cctcattgtg cggcctcctt
     8161 actgtatttc tcccacagcg gcttgatctc ttcttcccag acgcgaggat tgagatcgcc
     8221 cgcatggcga tagcgaatga tgccgttgcc gtcgatgagg aacgtttctg gcgcgccata
     8281 gacaccgaga tccagtccta acatgccgtc gccatcaaac aggcttagtg cgtaaggatt
     8341 gcccagctct ttcagccagc tgattgcctt ctggcgatcg tctttatagt tcatgccgac
     8401 cacgcggatg ccctgcgcag aaagctgatt cagatattga tgttccgcac ggcaggtcgg
     8461 acaccaggtc gcccagacgt taagcagcac tggtttgccc tgagtcagca catccgcctg
     8521 ataaaactgc cccggattgt ccagtgattc gaggcgaaac ttcggcactg gcttgccaat
     8581 gagcgccgat tccagattgg tcggatcatc cccttcggca ttacgcgcca gctgccacag
     8641 tagcgccgcg gcaatcgcca ggaagataat caacggaatt aacaatactt tgcgcttcat
     8701 acagcctccg gcgcagtttt ctgcgggttc acgcgcttac gatagcgagg atcaaacagg
     8761 cacagcagcc cgcccaatgc catcatcagc ccgcccgccc aaatccagcg aacaaacggt
     8821 ttgtagtaaa gacgcacggc ccacgcgccg ttttccagct cttcaccgag cgcggcgtac
     8881 aggtcacgcg tgatgccgcc gtcaatcgcc gcttcggtca tcatcgaccc ggcagtgttg
     8941 taataacgtt tttccgcata cagcaccgtt tccggcttgc catcgcgcgt tacgccgata
     9001 gtcgccacac cgccacgcca gttcgggcca gtcacctctt tgacatcacg gaaggtgaag
     9061 cgatattcat gaatatcgac actatcgccg gacttcatgc gcacatcacg ctcaacgcta
     9121 tagttctggc taaaggcaat accaacaatt gtcactgcca gccccaggtg agccgccacc
     9181 attccccaat aactgaaggt ggttttcgtg ccacgtgaaa tacgcagcgc agcttccgca
     9241 attgccagca ccgcaatcca gcaggccatt gccaggccga gcaccgtcat cgccacaact
     9301 ttgctttcga acagccacgg cagcagcagc gacagcacca gcgttgagat gaaggcgata
     9361 atcaataaat tgcggatttt acgtgggcga tcgcgccccc agcgcaccag aggaccgaca
     9421 ccaagcagca gcgcaaacgg caccatcagc caggtaaaca tggtgttgaa gaacggttcg
     9481 ccaatcgaaa tactgcccag tcccagttgc ttgtgcacca gcggcagcag cgtccccagt
     9541 aataccacca gcatcgcggc gaccagcaaa acgttgttcg ctaacagcag agattcccgc
     9601 gaccacagcg cattgtttac gcgtgagcga actttgtgtc cacgcgcggc aaacagcagc
     9661 agcgaaccac caatcaccag caccataaag gcgaggataa acataccgcg cgccggatca
     9721 gacgcaaacg cgtgtaccga taccagcacg ccggaacgca cgaggaaagt ccccaacaga
     9781 cacaacgaga aggcactgat tgccagcagt aatgtccacg ctttgaagct ggcgcgttgt
     9841 tcagtgaccg ccagtgagtg catcagcgca gtccccacca gccacggcat aaacgaggcg
     9901 ttttctaccg gatcccagaa ccaccagccg ccccagccga gttcgtaata ggcccatgcg
     9961 gaaccgagca cgatgccgag cgtcaggaag atccacgctg ccagtgtcca cggacgagta
    10021 aaacgcgcat aagtgctgtc cagacgcccg ctcaacaaag aagcaatggc aaaagcaaac
    10081 gccaccgaga agccgacgta ccccatataa agcagaggcg gatggaagat cagccccgga
    10141 tcctgtaaca gcgggttaag atcgcgacct tcaatcggga agttcggcaa cgtgcgtgag
    10201 aacgggttag aggtaaagag aatgaacagc aaaaagccga cgctgaccat ccccattatc
    10261 gccagtacgc gggccacaat atccagcgga atacgctgac taaaaatcgc caccgcaaag
    10321 gtccagccgc tcatcagcag cacccacagc agaagcgaac cttcatgcgc gccccaggta
    10381 gccgccacgc gataccacac cggaagctgg gtattggagt tgctggcaac ataggtgacg
    10441 gtaaagtcgt tgaccacgaa agcattgacc agcaccagaa atgcgccagc cacagacata
    10501 aacagcagcc aggcaaacaa gcgggaagac gccatcatgc gcgcatctcc gcgcgccacg
    10561 ccccatagcg gatacacgga cagcagcagc gcaatcccta acgccaggca cagcagcccg
    10621 ttaccaattt ctggcatcat gatgctgggt ccttataaac actcgccggg cgacggtggt
    10681 tagcttccat cgctttctca acttctggcg gcgtgtagtt ttcatcatgt ttcgccagca
    10741 cttctttcgc gaggatatga ttgccttttt ccagctcgcc ctgcaccaca acgccctgcc
    10801 cttcacggaa cagatccggc aaaatgcctt cgtaagagac atccactgaa ccttcagcat
    10861 cgtaaatggt gaaagttact ttcagcgaat tgggatcgcg ctgcacacta cccggcatca
    10921 ccatcccgcc aacgcgcaga cgctgaccga cttccggcat ttgctgagtt tcacgcttgc
    10981 cgtagagaat ttcccccggc gtataaaaga gatcgatatt cgagcgcagc gcatacagca
    11041 ccagaccgat agtaagcgcc agccccgcca aaacggcaca ggcaatccac aagcggtttt
    11101 tacggcgaat attcatgcag cctcctgctg ttgcgcagca cgtaaacgcg cctcacgcgc
    11161 ccgctgttgc gccacgccac gcagaattgc gcgatgctgc atcaccgagt gcacgactaa
    11221 aatcaccagc ggaataacgg tcatcgctac cgccagccag acaaaaaagg cgtaaccgcc
    11281 cattgcgaaa aattcattcc aggaagcaaa tgcaggggtc atttacggcc tcttttcagt
    11341 atcagttcac tcacccacgg acggcgtttt tccatcagca aaatcaaatt acgcatccgc
    11401 atcagcgtca gcgtggcaga cagcagcagg aagccaaaaa tcgaccagcg cagcggcgaa
    11461 cgcatcgccg gatcgatact ttgctgcatc cgcgttgatc cctgatgcag ggtgttccac
    11521 cactccacgg agtaatgaat aatcggcaga ttcaccacgc caatcagcac cagaatacct
    11581 gccgcacggc ccgccagacg gcggtcgtcg aaggcgtgcc acagggcaat tacaccaaca
    11641 tacagaaaca gcagcaccag ttcagaagtc aggcgtgcgt cccataccca ccaggtgccc
    11701 cacatcggtt ttccccatgc agagccggta accagggcaa taaaggtaaa cacggcacca
    11761 atgggggcca tcgccgctac cgccaggttg gccattttca tctgccagac aaggccaata
    11821 aacgccgcca ccgccattga tgcataaatg cccatcgacc agatcgccgc aggcacatgc
    11881 aggtagataa tgcggtagct atttccctgc tgataatcag ccggagcaaa gccgaatccc
    11941 cagatccagc cgacggtaag cacgaccaca ctggcaattg ccagccacgg tataaaccag
    12001 ccacagattt gatacagccg tggtgggatc gccagttgat gcagtgtttt ccacatagtt
    12061 tcgataccag actcgaacaa aaatcagtaa tccagcgtta ttgaatgctg attcgtaacg
    12121 ctgccgccgt cgcaaaagga cttaaagtcg cggtgcctgc cagcaacgcg cctaaaattg
    12181 ccagataccc gtcaacgggc aaatgcatgg aagccgcgtc catcgcggcg gtggcaaaga
    12241 tgagtaatgg gatagtcagc ggtaacacca gtatgctgag cagcacacca ccgcgcttaa
    12301 gtcccactgt cagcgccacg cccggtgcac cgagaaagcc aagcgtaggg gttcccagca
    12361 gcagcgtcag cgccatcact tgccagccat aaacatccat tcccagtagc attgctacca
    12421 ggggcgaaag gataagtaac ggcagaccgg ttaccatcca gtgcgccatc accttcgcca
    12481 gcacaacggc gggcaagggt aacggcaaca acatcaattg ttcaagactg ccgtcctgca
    12541 aatcgtcacg gaacagacgc tccagtgcca gcaaggatga aagcagtgcg gcaacccaga
    12601 taacgcccgg tgcaatacgc gccagcagtt gcggctccgg accgatactg agcggaaaaa
    12661 gggtaatgac aatcaggaag aaccacagcg ggttggcgat ttcggcgcta tggcgaaacg
    12721 ctacgcgcag ctcaagacgg aaaatgcgcc agaacatcat gcggccctcg tttgcgtcag
    12781 tgaaatgcgg cgaattttac tttcagcaac gttgagcggc tggtgggtag tcagaatcac
    12841 aatccccccc tgctccgtat gctgcgccat acgctgggtc agacgatcga cgccgttaac
    12901 gtcaatcgcg gtaaaaggct cgtcgaggat ccataacgtg gcacgggtca gccacagacg
    12961 cgctaaagcg acgcggcgtt gttgcccggc agaaagttga tttacaggaa tatcttcaaa
    13021 tccggcaagc ccagcctgcg ccagggcttc cagacattgt gcggtatcgc catcgcgatg
    13081 ataaaagtgc agattttcta acgccgtcag ccgggttttg atccccggct gatggcctat
    13141 ccataacagg ttttgatggt agctgtcgcg tacctgatgc aagggctgcc cttgccagag
    13201 aacgtcaccc gcatcagggc gagacaaccc cgtcagcaaa cggagaagcg ttgtcttccc
    13261 cgcgccgttg ctaccggtga tttgtaccca ctctcctgcg ttcagcgtaa atgacaagcc
    13321 actaaataag gttcgttcat cccgctcgca aagtaactct ctggcttcaa gcatacccac
    13381 gcaataaccc tgttaaaaac ctggctcgac ttcgcgcata tcgggcagct tgtgcgctat
    13441 ccctttatgg caatcaatac aggtttgccc atctttcacc gcctggtcat gcatcttcgc
    13501 ggcaaccgat ttctgggcgg ttgtatccat atactcgaag ttgtgacagt tacggcactc
    13561 ctgcgagtta ttgtccttca tgcgccgcca ctcattctgt gccatcgtca ggcgatgagc
    13621 ttcaaatttc tgcggcgtgt caataacgcc aaaaatttta ccatacagct ctttacttgc
    13681 tttgagcttg cgtatcatct tcggcacaaa ctcgtgcgga acgtgacaat ccggacaggt
    13741 cgcacggacg ccgctacggt tgttgtagtg cacggaatcc atgtattcct gatacaccgt
    13801 gttacgcatt tcgtggcagc taatgcagaa ctcttcggta ttggcttttt ccatcccggt
    13861 gttaaagccg ccccagaaga cgatgccgcc aacaaagccg atcaacagca gcgtccccag
    13921 cgccagacgg ctgggggtac gccaccattt ccacaggcgc ttaatcagac caggcttacg
    13981 gtcagaattt cccataataa cctcttattt cccgtaacct tttgacgggg taaaggtatt
    14041 ccccacgatt ggcgcggtat cggcctgcgg tacgtgacat tgcagacaga aataacgacg
    14101 cggagccact tccgcgccca ctttgccgtc gctgtccata aagtgagtag gactgatgcg
    14161 cggcgcgcca gtggtgcgat agctttcgac accgtggcac tgcaagcagc ggttggtatt
    14221 ggtcgttacc tgataacctt caacgctatg cgggatcatt ggcggctgat tcacatagtt
    14281 cagcggcatc cgttcctgct cttttggcat ccgaatggcc ccttcctgcg tcccggatac
    14341 ttccggcgac tggctaaaat cgacgccatt agccgcccaa accgcgccgc ttaccaccag
    14401 ggccagcatc gccgtccatt gacacagcgc tttcttcagg tcatggcttt tcatgatttc
    14461 gctcccgaac tccatcgtgt agttattgta aatacatcct cagaacagac atccacgcag
    14521 cgaccgcacg tcatgcaatc gcggctggtg acctgcaccg ggctttgttc atccagcacc
    14581 ggggcacgta gcacatgcgg ttccgggcaa acatgaaaac aatccataca gcggttacat
    14641 ttctgccgat cggtggccgc aacggtaatg acacctttgc tacccagcac gccatacagc
    14701 gcgccaaccg gacaaatgtg cccgcaccag ccgtgttcaa cgaccagtag atcaaataaa
    14761 aacagcgcga gaataagcag cgcgccgctg ccgaatccca ttaccagact gcgccccatc
    14821 agggaaacgg ggttgatcca ttcccagatg agcgtgccgg ttaaggccga tcccagcaga
    14881 atgaccacca acagcacgta gcgaatgtgg cgagggatcg tcgcagactg attgaggtca
    14941 aatcgtctgc gtaaccagtt cgctaagtcg gtaatcggat tcagcgggca gacccagctg
    15001 caaaataatc gctttcctgc cagcgcgtag agcacggtga taatcaccgc accggtcaac
    15061 gccaccgtgg caggcagatg accgctggca agactttgca gcgtcatcag cggatcggtc
    15121 agtggaacgg tgtcgaataa caggctactg ctgtagttgc cgtgcaagat ccacacacca
    15181 aaccacggac cgctcaaaaa catccccagc acgaagaact gacaaaggcg acgtaacacc
    15241 agccaacggt gactgcgcca ccagcctttt ttctccagcg cctcgcgccc ggcgtcacgt
    15301 ttacgatttg ccattgttcc cctccagcca gccgaagcgg taatggtgac ctaactcccc
    15361 tttcgccagt gacagcggta acaccttgat tgccggttgt tccagcacgc acactttttc
    15421 gcacttaccg cagccggtac aggcgtcgct gtgaaccgtc ggcagaaagc gggcgtgctt
    15481 accggtacgc gtgttgcgct ccagctccag ggtgattgcc tcatcaattt tcgggcattc
    15541 gcgataacaa acatcgcagc gcagcccctg aaagttgaga cagttttcct ggtccaccag
    15601 taccgccagc cccatccgcg cgtcgtcgat cgattcgatc tcacgatcca gcgcaccgct
    15661 cgggcacact ttggcgcacg gaatgtcctc acacatttcg caaggaatat cccgtgcgac
    15721 aaaatatggc gtgcccgccg acaggccaga agccagcgtc gccagtttta aggtgtcgta
    15781 agggcaagcc tgaacacact gaccgcaacg cacacaggca ctggcaaagg cgttctcgtt
    15841 tatggctccg ggcgggcgca accgcacgcc agatgcgcgt gcggtttgct gttgtaaccc
    15901 cagcgccaca cccacggcag ccagcccgcc agctgtgcga acgacatcgc gcagaaagcg
    15961 gcggcgacca ttttgaggtt tcgctgaccg ggacataatg gcttacacct tctccagttt
    16021 gaccgcgcac ttcttgaagt ccgtctcttt cgagagcgga tcggtcgcgt ccagcgtcag
    16081 tttgttaacc agctgtgcgg cgtcgaagaa cggcatgtac accaagccct gtggcggacg
    16141 gttacgaccg cgcgtttcaa caatcgagat cacttcgcca cggcgagaaa ccactttcac
    16201 tttgtcgcca cggcgcagat cgcgcgcttt cgcatccaac gggtgaataa acaggaccgc
    16261 ttccgggaag gcacggtgca gttccggtac acggcgagtc atactgccgg tatgccagtg
    16321 ctccaggacg cgtccggtag agagccacag gtcgtactct tcatccggtg cctccgccgc
    16381 cggttcgaac ggcagtgcga agatcaccgc tttgccatcc ggtttaccgt agaacttata
    16441 gccttcgccc gctttcacgt acgggtcgtt accttcgctg taacgccact gtgtttcttt
    16501 accgttcacc accggccagc gcagaccgcg cgctttgtgg tagtcatcga acggtgccag
    16561 atcgtgaccg tgaccgcgac caaaccatgc gtactcttcg aacagccctt tttgcagata
    16621 gaagcccagc tcgcgggatt catcgttcag ctgatcttcc gccagttcgg atacagggta
    16681 tttgctcact tccggggtgg catacagaac ttcgtacagc gttttgccac gcagttccgg
    16741 tttcttcgcc agcagatctt ccggccatac ttcttcagtt ttgaagcggc gggagaactg
    16801 gactaactgc cagagatccg atttcgcttc gcccggtgcc tgtacctgct gacgccagaa
    16861 ctgagtacgg cgttcggcgt taccgtaagc accctctttt tctacccaca ttgcggtcgg
    16921 caggatcaag tcggcggcca gcgcactgac tgtcggatac ggatcggaga cgatgatgaa
    16981 gttgcgcgga tcgcgccagc ccggcatacg ctcttcattg atgttcggcc cggcctgcat
    17041 gttgttggta cacatggtcc agtaaacatt cagcttgccg tctttcagcg cacggtcttg
    17101 tgctaccgca tgcagaccga ttttcgccgg aatggtgccg ctcgggatat tccacttctt
    17161 ctcgcagata tcacgatgtt tctcgttagt taccaccatg tccgcaggca gacggtgagc
    17221 aaaggtgccc acttcacgcg cagtaccaca cgcggaaggc tgcccggtca gggagaacgg
    17281 accgcaaccc ggcttggaaa ttttgccggt cagcaggtgc aggttgtaga ccaggttgtt
    17341 ggcccataca ccacgggtat gctggttgaa gcccatcgtc cagtaggaga tgactttctt
    17401 gttcggatcg gcatacagct gcgccagttg ttctaactgg tctttcggca cgccggtcat
    17461 ttcggcagtt ttttccagcg tatactcagc aacgaaggct ttgtaatctt caaagctcat
    17521 cggttcggag gcgtcagaac ctggattctt cgctgctttt tccagcggat gggtcggacg
    17581 taaaccgtag ccgatgtccg tcgccccttt gcgcaggtta acgtgtttgc taaagaagtc
    17641 ctgatttatc gcattgtttt gaatgatata gttggcgatg tagttcagga tcaccaggtc
    17701 tgattgcggc gtaaagatga tgccattatc cgccagctcg aagctacgat gctggtaggt
    17761 agaaagcacc gccacggtga cgttctggtt agagagacga cggttagtga tgcgcgacca
    17821 gaggatcggg tgcatttccg ccatgttcgc gccccacagc acaaacgcat cagcctgctc
    17881 gatgtcgtca tagcagccca tcggctcatc cataccaaag gtacgcataa agccaactac
    17941 cgccgacgcc atacagtgac gcgcgttcgg gtcgatgttg ttcgaacgga atcccgcttt
    18001 aaacagcttg gacgcggcat aaccttccca gatagtccac tgaccagaac cgaacatacc
    18061 gatcgattcc ggcccttttt ctttcagggc ggttttgaat ttctcttcca tcacatcgaa
    18121 ggcttgatcc caggtgattg gggtaaattc gccttctttg tcatatttac cgtttttcat
    18181 acgcagaagc ggctgcgtca aacggtcttt accgtacatg attttgggca ggaaatagcc
    18241 cttaatgcag ttcagaccac ggttaaccgg tgcgtccggg tcgccctgac aggccaccac
    18301 acgcccctgc tgcgttccga ccagaacgcc gcaaccagta ccgcagaaac ggcacggcgc
    18361 tttatcccat ttaatggctt cctgctgacc aacaacggcg cgggcaacgc ccggcacgct
    18421 gagaccggca gccgccgcag cggccgcaac ggcgttagct ttcataaagc tacgacgact
    18481 gagtttcatg gtgtttcctc accttgctct tcctgctggt gataaaccag cgacaccgcc
    18541 agcacgccct ctacgttgcg tactgactca atggtttgaa tcagcgtttc gctgtcttct
    18601 gcttccacca ccacaatcaa ctgaccgctc ggcgcgtcgc tgacagcaac ttcacagccg
    18661 ggaaaggcgt tcagttgggt gctgatgtct gaaattcgtt cgcttttggc ctgcacgacc
    18721 aggctgcaaa cttgccagtt agtgtgcatg gagatactcc gcagttatgg ctgataccgg
    18781 acagctggct gcacatgctc cacatccgtt gcaaagttgg ctattaagtt gcggctggta
    18841 gatcccggag agcgtcgggc gaaagataat tgccattggc tcacagctat cctgacagcg
    18901 gcggcattca actgtttgat acgccaggca cgcctccccg atggtgaaca gcaaatccca
    18961 ggccctggtg tggcgcggag aaaatagcga ttcggggcaa gcctgcgcgc aggcgtaaca
    19021 gaagctgcac tcgttatttt tgaaattaac actcggatag ccacccgcgc cgcgttgcag
    19081 aatgttgttt tcacaagcat taatacaggc gtcgcagcgg gtgcaatggg tcagaaaatg
    19141 agattcatca ccggaccagg gcggacggat accgttactg gctttgcgcc agcgaccagt
    19201 gagtatgccc cgacgggatg catcaatctt cacattgacc ttccatcatt aacgcgctct
    19261 gaaaattgag agcgaccaaa taaaccgcat aattaataag ccatttttat agccgctaag
    19321 atattaaagg atgtgtcaaa gatgcatacc ccgatcgggg taaaacctgt agcaggatca
    19381 aaagagtggc gggaagcgtg gcaaaaacgg gcttttgctc acatttcaaa tggttataaa
    19441 catatttata tagcgattaa ttcaccagaa atatttctgt tggtttgctc tctcatcaga
    19501 atttaacact aaaagagcag gtaaaattat ctgaatgttc tttaagttat tcataaagca
    19561 aattaataaa tctgatgaat atgttaacct tcagcgacat catcggtgaa aacctataaa
    19621 tgaagaagga aagcaaaaat gaagaccatt ctacctgcag tattgtttgc cgctttcgcc
    19681 accacctccg cctgggcggc agagagcgtc cagccactgg aaaaaatcgc gccttatccg
    19741 caagctgaaa aaggcatgaa gcgtcaggtg attcagttaa ccccgcaaga agatgaatct
    19801 accctgaaag tagaactgtt aatcggtcag acgctggaag tcgattgcaa tttgcatcgt
    19861 ctcggcggga agctggaaag caaaacgctg gaaggctggg gctatgatta ttatgtcttt
    19921 gataaagtca gttccccggt ttcgacgatg atggcctgcc cggatggcaa gaaagagaag
    19981 aaatttgtca ccgcgtatct gggcgatgcc ggaatgctgc gttataacag caagctgccg
    20041 atcgtggtgt atacgccaga caatgtggat gtgaagtacc gcatctggaa ggcggaagag
    20101 aaaattgaca acgcggtagt ccgctaaact gccgtgaagt gctccacccc gtaggtcgga
    20161 taaggcggtc acgccgcatc cgacatctaa cgcccaagcc ggttgcctga t
//
LOCUS       DAOTRK010000084        20097 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.084, whole genome shotgun sequence.
ACCESSION   DAOTRK010000084 DAOTRK010000000
VERSION     DAOTRK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.084"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(317..1129)
                     /locus_tag="QIE41_002902"
     CDS             complement(317..1129)
                     /locus_tag="QIE41_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDS3531771.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            1391..2152
                     /gene="udp"
                     /locus_tag="QIE41_002903"
     CDS             1391..2152
                     /gene="udp"
                     /locus_tag="QIE41_002903"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDS3531772.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRSELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2293..3720
                     /gene="rmuC"
                     /locus_tag="QIE41_002904"
     CDS             2293..3720
                     /gene="rmuC"
                     /locus_tag="QIE41_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDS3531773.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQKIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3815..4570
                     /gene="ubiE"
                     /locus_tag="QIE41_002905"
     CDS             3815..4570
                     /gene="ubiE"
                     /locus_tag="QIE41_002905"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDS3531774.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4584..5189
                     /gene="ubiJ"
                     /locus_tag="QIE41_002906"
     CDS             4584..5189
                     /gene="ubiJ"
                     /locus_tag="QIE41_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDS3531775.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5186..6826
                     /gene="ubiB"
                     /locus_tag="QIE41_002907"
     CDS             5186..6826
                     /gene="ubiB"
                     /locus_tag="QIE41_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDS3531776.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6905..7174
                     /gene="tatA"
                     /locus_tag="QIE41_002908"
     CDS             6905..7174
                     /gene="tatA"
                     /locus_tag="QIE41_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDS3531777.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7178..7693
                     /gene="tatB"
                     /locus_tag="QIE41_002909"
     CDS             7178..7693
                     /gene="tatB"
                     /locus_tag="QIE41_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDS3531778.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7696..8472
                     /gene="tatC"
                     /locus_tag="QIE41_002910"
     CDS             7696..8472
                     /gene="tatC"
                     /locus_tag="QIE41_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDS3531779.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8514..9296
                     /gene="tatD"
                     /locus_tag="QIE41_002911"
     CDS             8514..9296
                     /gene="tatD"
                     /locus_tag="QIE41_002911"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDS3531780.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARALDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLFPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAAITDANVKTLFGIAF"
     gene            complement(9293..9781)
                     /gene="rfaH"
                     /locus_tag="QIE41_002912"
     CDS             complement(9293..9781)
                     /gene="rfaH"
                     /locus_tag="QIE41_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDS3531781.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKMVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9948..11441
                     /gene="ubiD"
                     /locus_tag="QIE41_002913"
     CDS             9948..11441
                     /gene="ubiD"
                     /locus_tag="QIE41_002913"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDS3531782.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIISGDDVDLHRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11487..12188
                     /gene="fre"
                     /locus_tag="QIE41_002914"
     CDS             11487..12188
                     /gene="fre"
                     /locus_tag="QIE41_002914"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDS3531783.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12379..13542)
                     /gene="fadA"
                     /locus_tag="QIE41_002915"
     CDS             complement(12379..13542)
                     /gene="fadA"
                     /locus_tag="QIE41_002915"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDS3531784.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRQVRAEDLSAHLMRSLLARNPT
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     NREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(13552..15741)
                     /gene="fadB"
                     /locus_tag="QIE41_002916"
     CDS             complement(13552..15741)
                     /gene="fadB"
                     /locus_tag="QIE41_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDS3531785.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAIMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKRAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15931..17262
                     /gene="pepQ"
                     /locus_tag="QIE41_002917"
     CDS             15931..17262
                     /gene="pepQ"
                     /locus_tag="QIE41_002917"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDS3531786.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17262..17876
                     /locus_tag="QIE41_002918"
     CDS             17262..17876
                     /locus_tag="QIE41_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDS3531787.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17915..19366
                     /gene="trkH"
                     /locus_tag="QIE41_002919"
     CDS             17915..19366
                     /gene="trkH"
                     /locus_tag="QIE41_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDS3531788.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19378..19923
                     /gene="hemG"
                     /locus_tag="QIE41_002920"
     CDS             19378..19923
                     /gene="hemG"
                     /locus_tag="QIE41_002920"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDS3531789.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKSTLK"
BASE COUNT         4681 a         5080 c         5481 g         4855 t
ORIGIN      
        1 aagggatgaa ctaaaatgtg aaaaacaaaa tgtttcactt taatatcttt ttatcaggaa
       61 ggtctgagat aatttataat taatgtgaat gtgttgataa taattaatgt ttgtttgaga
      121 atgggagttg atggtaaatc tctaaggtgt ctgccagtga agagactcac ttatgactat
      181 tacttgccga ctttcttaag gtcaaatagc ggagtcgtgc tctccaatca attcatactg
      241 attatttttt gggggacaga gatggcagca aaacgcactc gttcactcac catctcaaat
      301 atagactaag aggaaactaa ttccgcttcc caccatactg cttaaaccac tccagcatac
      361 gctgccagcc atctttcgca gattcggcat gatagctcgg gcgataatcg gcgttgaatg
      421 catgcccggc atccgggtag acgataatct ctgctttcgc attagcagcc cgcagcgcct
      481 ggcgcatggt ttcaacgctc tcctgcggaa tgctgttatc ctgaccacca tataagccga
      541 gaaccggcgc gttaagatcg gttgcgatat caacaggttg tttcggtgaa ttcagcgact
      601 tatcgcctgt cagtttgccg taccatgcca ccgccgcttt cagttgcggg ttatgcgcag
      661 catacagcca ggtgatacgt ccaccccagc agaatccggt gatcattaaa cgatgaacat
      721 cgccgccgtt gcgggaagcc caactggcga catgatcgag atcggccagc acctgcgagt
      781 caggcacttt tgctaccaga ccgctaagta acgtggggat atcggcaaaa tcattcggat
      841 cgccttcgcg gaagtaaagt tcaggtgcga tagccagata cccctccagc gccagacggc
      901 gacaaatgtc gcggatatgt tcatgcacgc caaaaatttc ctgcactaca atgaccactg
      961 gcagtgggcc atcgctttgc tttggtctgg catggtaagc aggcatgtta tccccttgtg
     1021 aagggataga ggtgaagccc gccacaatcg cgtcgtccag ggtctgaacg atggtcgaag
     1081 ctagaggcga tgcagcaggt gcaaatccag attgttgtgt tgttgccatg gtattctccg
     1141 tacccttata aaaatgttgc gcaatgttaa ctatagtcag catgcaacaa atcacattgc
     1201 ctgaatcggc tcatctttta tgcagtcctg cagaatgaag agtgatttat gtgatttgca
     1261 tcacttttgg tgggtaaatt tatgcaacgc atttgcgtca tggtgatgag tatcacgaaa
     1321 aaatgttaaa cccttcggta aagtgtcttt ttgcttcttc tgactaaacc gattcacaga
     1381 ggagttgtat atgtccaagt ctgatgtttt tcatctcggc ctcactaaaa acgatttaca
     1441 aggggctacg cttgccatcg tccctggcga cccggatcgt gtggaaaaga tcgccgcgct
     1501 gatggataag ccggttaagc tggcatctca ccgcgaattc accacctggc gttcagagct
     1561 ggatggtaaa cctgttatcg tctgctctac cggtatcggc ggcccgtcta cctctattgc
     1621 tgttgaagag ctggcacagc tgggcattcg caccttcctg cgtatcggta caacgggcgc
     1681 tattcagccg catattaatg tgggtgatgt cctggttacc acggcgtctg ttcgtctgga
     1741 tggcgcgagc ctgcacttcg caccgctgga attcccggct gtcgctgatt tcgaatgtac
     1801 gactgcgctg gttgaggctg cgaaatccat cggcgcgacg actcacgttg gcgtgacggc
     1861 ttcttctgat accttctacc caggtcagga acgttacgat acttactctg gtcgcgtagt
     1921 tcgtcacttt aaaggttcta tggaagagtg gcaggcgatg ggcgtaatga actatgaaat
     1981 ggaatctgca accctgctga ccatgtgtgc aagtcagggc ctacgtgccg gtatggtagc
     2041 gggtgttatc gttaaccgca cccagcaaga gatcccgaat gctgagacga tgaaacaaac
     2101 tgaaagccaa gcggtgaaaa tcgtggtgga agcggcgcgt cgtctgctgt aatcctcttc
     2161 tcctgtctga aggccgacgc gttcggcctt ttgtattttt gcgtagcgcc tcgcaggaaa
     2221 tgcctttcca actggacgtt tgtacagcac aattctattt tgtgcgggta agttgttgcg
     2281 tcaggaggcg ttgtggattt ctcaatcatg gtttacgcag ttattgcgtt ggtgggtgtg
     2341 gcaattggct ggctgtttgc cagttatcaa catgcgcagc aaaaagccga gcaattagct
     2401 gaacgtgaag agatggtcgc ggagttaagc gcggcaaaac aacaaattac ccaaagcgag
     2461 cactggcgcg ctgagtgcga gttactcaat aacgaagtgc gcagcctgca aagtattaac
     2521 acctctctgg aggccgatct gcgtgaagta accacgcgga tggaagccgc acagcaacat
     2581 gctgacgata aaattcgcca gatgattaac agcgagcagc gcctcagtga gcagtttgaa
     2641 aacctcgcca accgtatttt tgagcacagc aatcgccggg ttgatgagca aaaccgtcag
     2701 agtctgaaca gcctgttgtc gccgctacgt gaacaactgg acggtttccg ccgtcaggtt
     2761 caggacagct ttggtaaaga agcacaagaa cgccataccc tgacccacga aattcgcaat
     2821 ctccagcagc tcaacgcgca aatggcccag gaagcgatca acctgacgcg cgcgctgaaa
     2881 ggcgacaata aaacccaggg caactggggc gaggtagtat tgacgcgggt gctggaggct
     2941 tccggtctgc gtgaagggta tgaatatgaa acccaggtca gcatcgaaaa tgacgcccgc
     3001 tcgcggatgc agccggatgt catcgtgcgg ttgccgcagg gaaaagatgt ggtgatcgac
     3061 gccaaaatga cgctggtcgc ctatgaacgc tatttcaacg ccgaagatga ctacacccgc
     3121 gaaagcgcgc tacaggaaca tatcgcgtcg gtgcgtaacc atatccgttt gctgggacgc
     3181 aaagattatc aacagctgcc ggggctgcga accctggatt acgtgctgat gtttattccc
     3241 gttgaacccg cttttttact ggcgcttgac cgccagccgg agctgatcac cgaagcgttg
     3301 aaaaacaata tcatgctggt tagcccgacc acgctgctgg tggcgctgcg cactatcgcc
     3361 aacctctggc gctatgagca tcaaagtcgt aacgcccaga aaatagccga tcgcgccagc
     3421 aagctgtacg acaagatgcg tttgttcgtc gatgacatgt cagcgattgg tcaaagtctc
     3481 gacaaagcgc aggataacta tcgccaggca atgaaaaaac tctcttcagg tcgcggaaat
     3541 gtgctggcgc aggcagaagc gtttcgcggt ttaggggtag aaattaaacg cgagattaat
     3601 ccggatttgg ctgaacaagc ggtgagccag gatgaagagt atcgactgcg gtcggttccg
     3661 gagcagccga atgatgaagc ttatcaacgc gatgatgaat ataatcagca gtcgcgctag
     3721 cccattgggg gtagttaagc cgggtagaaa tctagggcat cgacgcccaa tctgttacac
     3781 ttctggaaca attttttgat gagcaggcat tgagatggtg gataagtcac aagaaacgac
     3841 gcactttggt tttcagaccg tcgcgaagga acaaaaagcg gatatggtcg cccacgtttt
     3901 ccattccgtg gcatcaaaat acgatgtcat gaatgatttg atgtcatttg gtattcatcg
     3961 tttgtggaag cgattcacga ttgattgcag cggcgtacgc cgtgggcaga ccgtgctgga
     4021 tctggctggt ggcaccggcg acctgacagc gaaattctcc cgcctggtcg gagaaactgg
     4081 caaagtggtc cttgctgata tcaatgaatc catgctcaaa atgggccgcg agaagctgcg
     4141 taatatcggt gtgattggca acgttgagta tgttcaggcg aacgctgagg cgctgccgtt
     4201 cccggataac acctttgatt gcatcactat ttcgtttggt ctgcgtaacg tcaccgacaa
     4261 agataaagca ctgcgttcaa tgtatcgcgt gctgaaaccc ggcggccgcc tgctggtgct
     4321 tgagttctcg aagccaatta tcgagccgct gagcaaagcc tatgatgcat actccttcca
     4381 tgtgctgccg cgtattggct cattggtcgc gaacgacgcc gacagctacc gttatctggc
     4441 agaatccatc cgtatgcatc ccgatcagga taccctgaaa gccatgatgc aggatgccgg
     4501 attcgaaagt gtcgactact acaatctgac ggcaggggtt gtggcgctgc atcgtggtta
     4561 taagttctga caggagaccg gaaatgcctt ttaaaccttt agtgacggca ggaattgaaa
     4621 gtctgctcaa caccttcctg tatcgctcac ccgcgctgaa aacggcccgc tcgcgtctgt
     4681 tgggcaaagt gctgcgcgtg gaggtaaaag gcttttcgac atcattgatt ctggtgttca
     4741 gcgaacgcca ggttgatgta ctgggcgaat gggcaggcga tgctgactgc accgttatcg
     4801 cctacgccag cgtgttgccg aaacttcgcg atcgccagca gcttgccgca ctgattcgca
     4861 gtggtgagct ggaagtacag ggcgatattc aggtggtgca aaacttcgtt gcgctggcag
     4921 atctggcaga gttcgaccct gcggaactgc tggcccctta taccggtgat atcgccgctg
     4981 aaggtatcag caaagccatg cgcggaggcg caaagttcct gcatcacggc attaagcgcc
     5041 agcaacgtta tgtggcagaa gccattactg aagagtggcg tatggcacct ggtccgcttg
     5101 aagtggcctg gtttgcggaa gagacggctg ccgtcgagcg tgctgttgat gccctgacca
     5161 aacggctgga aaaactggag gctaaatgac gccaggtgaa gtacggcgcc tatatttcat
     5221 cattcgcact tttttaagct acggacttga tgaactgatc cccaaaatgc gtatcaccct
     5281 gccactacgg ctatggcgat actcattatt ctggatgcca aatcggcata aagacaaacc
     5341 tttaggtgag cgactacgac tggccctgca agaactgggg ccggtatgga tcaagttcgg
     5401 gcaaatgtta tcaacccgcc gcgatctttt tccgccgcat attgccgatc agctggcgtt
     5461 attgcaggac aaagtcgctc cgtttgatgg caagctggcg aagcagcaga ttgaagctgc
     5521 aatgggcggc ttgccggtag aagcgtggtt tgacgatttt gaaatcaagc cgctggcttc
     5581 tgcttctatc gcccaggttc ataccgcgcg attgaaatcg aatggtaaag aggtggtgat
     5641 taaagtcatc cgcccggata ttttgccggt tattaaagcg gatctgaaac ttatctaccg
     5701 tctggctcgc tgggtgccgc gtttgctgcc ggatggtcgc cgtctgcgcc caaccgaagt
     5761 ggtgcgcgag tacgaaaaga ccttgattga tgaactgaat ttgctgcggg aatctgccaa
     5821 cgccattcag cttcggcgca attttgaaga cagcccgatg ctctacatcc cggaagttta
     5881 ccctgactat tgtagtgaag ggatgatggt gatggagcgc atttacggca ttccggtgtc
     5941 tgatgttgca gcgctggaga aaaacggcac taacatgaaa ttgctggcgg aacgcggcgt
     6001 gcaggtgttc ttcactcagg tctttcgcga cagctttttc catgccgata tgcaccctgg
     6061 caacatcttc gtaagctatg aacacccgga aaacccgaaa tatatcggca ttgattgcgg
     6121 gattgttggc tcgctaaaca aagaagataa acgctatctg gcagaaaact ttatcgcctt
     6181 ctttaatcgc gactatcgca aagtggcaga gctacacgtc gattctggct gggtgccacc
     6241 agataccaac gttgaagagt tcgaatttgc cattcgtacg gtctgtgaac ctatctttga
     6301 gaaaccgctg gccgaaattt cgtttggaca tgtactgtta aatctgttta atacggcgcg
     6361 tcgcttcaat atggaagtgc agccgcaact ggtgttactc cagaaaaccc tgctctacgt
     6421 cgaaggggta ggacgccagc tttatccgca actcgattta tggaaaacgg cgaagccttt
     6481 cctggagtcg tggattaaag atcaggtcgg tattcctgcg ctggtgagag catttaaaga
     6541 aaaagcgccg ttctgggtcg aaaaaatgcc agaactgcct gaactggttt acgacagttt
     6601 gcgccagggc aagtatttac agcacagtgt tgataagatt gcccgcgagc ttcagtcaaa
     6661 tcatgtacgt cagggacaat cgcgttattt tctcggaatt ggcgctacgt tagtattaag
     6721 tggcacattc ttgttggtca gccgacctga atgggggctg atgcccggct ggttaatggc
     6781 aggtggtctg atcgcctggt ttgtcggttg gcgcaaaaca cgctgatttt ttcatcgctc
     6841 aaggcgggcc gtgtaacgta taatgcggcc ttgtttaatc atcatctacc acagaggaac
     6901 atgtatgggt ggtatcagta tttggcagtt attgattatt gccgtcatcg ttgtactgct
     6961 ttttggcacc aaaaagctcg gctccatcgg ttccgatctt ggtgcgtcga tcaaaggctt
     7021 taaaaaagca atgagcgatg atgaaccaaa gcaggataaa accagtcagg atgctgattt
     7081 tactgcgaaa actatcgccg ataagcaggc ggatacgaat caggaacagg ctaaaacaga
     7141 agacgcgaag cgccacgata aagagcaggt gtaatccgtg tttgatatcg gttttagcga
     7201 actgctattg gtgttcatca tcggcctcgt tgttctgggg ccgcaacgac tgcctgtggc
     7261 ggtaaaaacg gtcgcgggct ggattcgcgc gttgcgttca ctggcgacaa cggtgcagaa
     7321 cgaactgacc caggagttaa aactccagga gtttcaggac agtctgaaaa aggttgaaaa
     7381 ggcgagcctc actaacctga cgcccgaact gaaagcgtcg atggatgaac tacgccaggc
     7441 cgcggagtcg atgaagcgtt cctacgttgc aaacgatcct gaaaaggcga gcgatgaagc
     7501 gcacaccatc cacaacccgg tggtgaaaga caatgaagct gcgcatgaag gcgtaacgcc
     7561 tgctgctgca caaacgcagg ccagttcgcc ggaacagaag ccagaaacca cgccagagcc
     7621 ggtggtaaaa cctgctgcgg acgctgaacc gaaaaccgct gcaccttccc cttcgtcgag
     7681 tgataaaccg taaacatgtc tgtagaagat actcaaccgc ttatcacgca tctgattgag
     7741 ctgcgtaagc gtctgctgaa ctgcattatc gcggtgatcg tgatattcct gtgtctggtc
     7801 tatttcgcca atgacatcta tcacctggta tccgcgccat tgatcaagca gttgccgcaa
     7861 ggttcaacga tgatcgccac cgacgtggcc tcgccgttct ttacgccgat caagctgacc
     7921 tttatggtgt cgctgattct gtcagcgccg gtgattctct atcaggtgtg ggcgtttatc
     7981 gccccagcgc tgtataagca tgaacgtcgc ctggtggtgc cgctgctggt ttccagctct
     8041 ctgttgtttt atatcggcat ggcgttcgcc tactttgtgg tctttccgct ggcatttggc
     8101 ttccttgcca ataccgcgcc ggaaggggta caggtatcca ccgacatcgc cagttattta
     8161 agcttcgtta tggcgttgtt tatggcgttt ggtgtctcct ttgaagtgcc tgtggcaata
     8221 gtgctgctgt gctggatggg gattacctcg ccagaagact tacgcaaaaa acgcccgtat
     8281 gtgctggttg gtgcattcgt tgtcgggatg ctgctgacac cgccggatgt cttctcgcaa
     8341 acgctgttgg cgatcccgat gtactgtctg tttgaaatcg gtgtcttctt ctcacgcttt
     8401 tacgttggta aagggcgaaa tcgggaagag gaaaacgacg ctgaagcaga aagcgaaaaa
     8461 actgaagaat aaattcaacc gcccgtcagg gcggttgtca tatggagtac aggatgtttg
     8521 atatcggcgt taatttgacc agttcgcaat ttgcgaaaga ccgtgatgat gttgtagcac
     8581 gcgctcttga cgcgggagtt aatgggctac tcatcaccgg tactaacctg cgtgaaagcc
     8641 agcaggcgca aaagctggcg cgtcagtatt catcctgttg gtcaacggcg ggcgtacatc
     8701 ctcacgacag cagccagtgg caagctgcga ctgaagaagc gattattgag ctggccgcgc
     8761 agccagaagt ggtggcgatt ggtgaatgtg gtctcgactt taaccgcaac ttttcgacgc
     8821 cggaagagca ggaacgcgct tttgttgccc agctacgcat tgccgcagaa ttaaacatgc
     8881 cggtatttat gcactgtcgc gatgcccacg agcggtttat gacattgctg gagccgtggc
     8941 tggataaact gcctggtgcg gttcttcatt gctttaccgg cacacgcgaa gagatgcagg
     9001 cgtgcgtggc gcatggaatt tatatcggca ttaccggttg ggtttgcgat gaacgacgcg
     9061 gactggagct gcgggaactt ttgccgttga ttccggcgga aaaattgctg atcgagactg
     9121 atgcgccgta tctgttccct cgcgatctca cgccaaagcc atcatcccgg cgcaacgagc
     9181 cagcccatct gccccatatt ttgcaacgaa ttgcgcactg gcgtggagaa gatgccgcat
     9241 ggctggctgc catcacggat gccaatgtta agacactgtt tgggattgcg ttttagagtt
     9301 tgcggaactc ggtgttcttc acactgtgct taatctcttt attgataaga ttaagtaaca
     9361 gcatggagcg agcctcgccg tcgggttcgg tgaaaatggc ctgaaaacct tcgaacgcgc
     9421 cttcggtaat aatcacctta tcacccggat aaggggttgc cggatcgaca atgtctttcg
     9481 gtttatatac cgatagctga tgaatcaccg ccgatgggac tatcgctggc gatgcgccaa
     9541 agcgcacgaa gtggctgaca ccgcgggtcg cgttgatagt cgtggtatga atcacttctg
     9601 gatcaaattc cacaaacagg tagttgggga acaatggttc actgactgca gtacgtttgc
     9661 cacgcaccat tttttccagg gtgatcatcg gtgccaggca attcacagcc tgtctttcga
     9721 ggtgttcctg ggcacgttga agttgcccgc gcttgcagta cagtaaatac caggattgca
     9781 taatgactct tatccgttta atcggggcgc aaggatagca aaagctttac gctaagttaa
     9841 ttatattccc cggtttgcgt tataccgtca gagttcacgc taatttaaca aatttacagc
     9901 atcgcaaaga tgaacgccgt ataatgggcg cagattaaga ggctacaatg gacgccatga
     9961 aatataacga tttacgcgac ttcctgacgc tgcttgaaca gcagggtgag ctaaaacgta
    10021 tcacgctccc ggtggatccg catctggaaa tcactgaaat tgctgaccgc actttgcgtg
    10081 ccggtgggcc tgcgctgttg ttcgaaaacc ctaaaggcta ctcaatgccg gtgctgtgca
    10141 acctgttcgg tacgccaaag cgcgtggcga tgggcatggg gcaggaagat gtttctgcgc
    10201 tgcgtgaagt cggtaaatta ttagcctttc tcaaggaacc tgaaccgcca aaaggttttc
    10261 gcgacctgtt tgataaactg ccgcagttca agcaagtatt gaacatgccg acaaagcgac
    10321 tgcgtggtgc gccgtgtcag caaaaaatta tctccggcga tgacgtcgat cttcaccgta
    10381 tccccattat gacctgctgg ccggaagacg ccgcgccgct gattacctgg gggttgaccg
    10441 tgacgcgtgg cccgcataaa gagcggcaga atctgggtat ttatcgtcaa cagttgattg
    10501 gtaaaaataa actgattatg cgctggttgt cgcatcgcgg cggcgcgctg gatttccagg
    10561 agtggtgtgc ggcgcatccg ggcgaacgtt tcccggtttc tgtggcgctg ggtgccgatc
    10621 ccgccacgat tctcggtgca gtcacccccg ttccggatac gctttcagag tatgcgtttg
    10681 ccggattgct gcgcggcacc aaaaccgaag tagtaaagtg tatttccaat gatctcgaag
    10741 tgcccgccag tgcggagatt gtgctggaag ggtatatcga acaaggcgaa actgcgccgg
    10801 aagggccgta tggcgaccac accggttact ataacgaagt cgatagtttc ccggtattta
    10861 ctgtgacgca tattacccag cgtgaagatg cgatttatca ttccacctat accgggcgtc
    10921 cgccggatga acctgcggtg ctgggtgtcg cactgaacga agtgtttgtg ccgattctgc
    10981 aaaaacagtt cccggaaatt gtcgattttt acctgccgcc ggaaggctgc tcttatcgcc
    11041 tggcggtagt gacaatcaaa aaacagtacg ccggacacgc gaagcgcgtc atgatgggcg
    11101 tctggtcgtt cttacgccag tttatgtaca ctaaatttgt gatcgtttgc gatgatgacg
    11161 tcaacgcacg cgactggaac gatgtgattt gggcgattac tacccgtatg gacccggcgc
    11221 gggatactgt tctggtagaa aatacgccta ttgattatct ggattttgcc tcgcctgtct
    11281 ccgggctggg ttcaaaaatg gggctggatg ccacgaataa atggccgggg gaaacccagc
    11341 gtgaatgggg acgtcccatc aaaaaagatc cagatgttgt cgcgcatatt gacgccatct
    11401 gggatgaact ggctattttt aacaacggta aaagcgcctg atgcgcgttt gttttgccct
    11461 atttatcgat ccgacagaga aagcgcatga caaccttaag ctgtaaagtg acctcggtag
    11521 aagctatcac ggataccgta tatcgtgtcc gcatcgtgcc agacgcggcc ttttcttttc
    11581 gtgctggtca gtatttgatg gtagtgatgg atgagcgcga caaacgtccg ttctcaatgg
    11641 cctcgacgcc ggatgaaaaa gggtttatcg agctgcatat tggcgcttct gaaatcaacc
    11701 tttacgcgaa agcagtcatg gaccgcattc ttaaagatca tcaaatcgtg gtcgatattc
    11761 cgcacggtga ggcatggctg cgcgatgatg aagaacgtcc aatgattctc atcgcgggcg
    11821 gcaccgggtt ctcttatgcg cgctcgattt tgctgacggc gctggcgcgt aacccaaacc
    11881 gtgatatcac catttactgg ggcgggcgtg aagagcagca tctttatgat ctctccgagc
    11941 ttgaggcgct ttcgcttaag catccaggtt tgcaagtggt gccggtggtt gaacaaccgg
    12001 aagcgggctg gcgtgggcgt actggcaccg tgttaacggc ggtattgcag gatcatggta
    12061 cgctggcaga gcatgatatc tatattgccg gacgttttga gatggcgaaa attgcccgtg
    12121 acctgttttg cagtgagcgt aatgcgcggg aagatcgcct gtttggcgat gcgtttgcat
    12181 ttatctgaga tataaaaaac ccgcccctga caggcgggaa gaacggcaac taaaacttta
    12241 tacagtggca tttagatcta tgtcgcatcc ggcattcggc acacgatgcc tgatgcgacg
    12301 ctggcgcgtc ttatcaggcc tacattggtg ccggatcggt agaccggata aggcgttcac
    12361 gccgcattcg gcaagtggtt aaatccgctc aaacaccgtc gcaataccct gacccagacc
    12421 gatacacatc gtcgctagac caaactgggc atctttgcgc tccatcagat tcagcagcgt
    12481 ggtgctgata cgcgcaccgg aacaacccag cggatgaccc agcgcgatcg cgccaccgtt
    12541 gaggttgatc ttctcgtcaa tctgctcaat cagtcccaga tctttaatac atggcaggat
    12601 ctgcgcggca aaggcttcat tcatttcaaa cacgccgata tcactgacag aaagccccgc
    12661 ttttttcagt gccagcttcg aggccggaac cgggccgtaa cccataatcg atgggtcaca
    12721 accaacgacc gccatcgaac gcacgcgggc gcgcggctta agacctaatt catgggcgcg
    12781 gctttcactc atcaccagca tggcagctgc gccatcggaa agcgcagaag atgtacccgc
    12841 cgttaccgta ccgctgacgg gatcaaacgc cggacgcagc gtggcgaggg cttccacggt
    12901 ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg
    12961 accaccggtc gggataattt catttttaaa tgcacccgac tgcgtggcag cccaggcgcg
    13021 ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg ttgataccgt gcatacgcgc
    13081 cagcatttct gccgttaagc ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc
    13141 ggggtgaaaa tcgacgccgt gactcatcgg cacatggccc atatgctcca cgccgccaac
    13201 cagacatgcc tgcgcatcgc cggtcatgat cattcgtgct gcatcatgca gtgcctgcat
    13261 ggatgaacca cacaagcgat tgacggtaac cgccgggaca gagtgtggta cttctgccag
    13321 cagcgccgcg ttacgggcga tattaaaacc ctgctccagc gtctgctgca cacaacccca
    13381 gtaaatatcg tcgagggccg ccgcttccag cgtcgggtta cgcgccagca ggctacgcat
    13441 taaatgagcg gagagatctt ctgcacgcac ttgacgaaaa gcaccgccct tcgaacggcc
    13501 catcggggtg cgaattgcat cgacaatgac aacctgttcc attgtgactc cttaagccgt
    13561 tttcaggtcg ccaaccggac gggctggctc aaccggggga tagtacggtt cgttatgacg
    13621 cgctttatta cgcagacctt ccggcacttc atacagcggg ccgaggtgct gatattgctg
    13681 tgccatatcg aggtattttg cgctaccgag ggtgtccagc cagcggaacg cgccaccgtg
    13741 gaacggaggg aagcccaggc cgtagaccag cgccatatcc gcttctgccg gagtggcgat
    13801 aatgccttct tccagacagc gcaccacttc gttgaccatc gggatcatca tgcgggcgat
    13861 aatctcttct tcgctgaaat cgcgcttcgg ctggctcact tctgccagca ggtcgtcaac
    13921 agcggcgtct tcttctttct tcggcttacc tttgctgtct tctttataac gccagaaacc
    13981 gaggccgttc ttctgaccaa agcggttggc atcaaacagc gcgtcgatgg catcgcggta
    14041 atctttctgc atccgctgcg ggaagcctgc tgccatgaca gcctgggcat gatgcgcggt
    14101 gtcaatgccc acaacgtcca gcagatatgc cggacccata ggccagccaa actgtttttc
    14161 catcactttg tcgatcttgc ggaaatccgc gccgtcgcgc agcagctggc tgaaacccgc
    14221 gaagtagggg aacagcacgc ggttaacgaa gaatccgggg cagtcgttaa ccacaatcgg
    14281 cgttttgccc atcttgctcg cccaggcgac gactttcgcg atggtttcgt cggagctttt
    14341 ctcgccgcga ataatttcta ccaacggcat ccggtgtacc gggttaaaaa agtgcattcc
    14401 gcagaagttt tccggacgtt ccagcgcgtt ggccagctcg ctgataggaa tggttgaagt
    14461 gttagacgcc agcacggtat ccgggcgtac tttttgctcg gtttctgcca gcacggcttt
    14521 tttcactttc gggttttcga caaccgcttc taccacaaca tccacgcggt caaatccggc
    14581 gtagtcgagc gttgggtgga ttgtggagat cacgccagcc agtttcagac catcgatctt
    14641 gccgcgctca agctgcttgt tcagcagttt cgcggcttca gtcatgccga gggttaacga
    14701 cttgtcgttg atatctttca tgataaccgg cacacctttc cacgcagact ggtaagcgat
    14761 gccgccgccc ataatgcctg cgcccagcac agcggcccgt ttcggcgttt caacgtcttt
    14821 ggtgagtttc ttcgctttgc cttttacata ttgatcgtta aggaatatgc cgaccaatgc
    14881 gcgagcttcg ttggtatgcg ccagcgggac aaaacttttg ttttccaggt ttagggcttc
    14941 ttcacgacca aaacgggccg cagcttcaat ggtttttact gcggtgatgg gggccggata
    15001 atgtttcccc gccgtttgtg cgaccatccc tttagcgatg gtgaagctca tgatggcttc
    15061 aatcttgctc agttttagcg gttccagctt cggctgacgt ttcgctttcc agtcgaggtc
    15121 gccgttaatg gcctggcgta aaatcgccat cgccccttca atcagttttt ctgctttgac
    15181 tacaccatcc accagaccga ttttcagcgc ctgatccgcg ccgacatctt taccggcggc
    15241 aatgatttcc agcgcactgt cagcgcccag catacgtggc atacgtacag aaccgccaaa
    15301 gcccggcatg atgcccagtt tggtttccgg cagaccgatg cgcagatccg gcgtcgccag
    15361 acgataatcg gtcgccagca cgcattcgca gccaccgccc agcgcatagc cgttgacggc
    15421 agcaatggtc ggcaccggga gatcttccag gcgattaaac acgctattgg caaagtgcag
    15481 ccactgactt aactgttctt caggaacgag gaacagggac aaaaattcgg tgatatcagc
    15541 accgacgata aaggctgctt tgttcgaacg cagcagcagc ccttttagat ctgattgctg
    15601 ttccagcacg ccgatggcct cgccgaggct ggcgacggtc gcagtgtcga gtttattaac
    15661 tgaacctggg gcatcaaata ccagttcggc aatgccatct tccagccagt caaggtacag
    15721 ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca
    15781 gatgcatcga agtgtgtatt ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc
    15841 gatctgatca cgctcggcag aaatcacgct ctggatgaac gatgtgctaa gatgcggaga
    15901 cttaaggtca aaaaaacaga agggtaaaaa atggaatcac tggcctcact ctataaaaat
    15961 catatagcta ccttacaaga acggactcgc gatgcgctgg cgcgcttcaa gctggatgcg
    16021 ttacttattc actccggcga actgttcaac gtttttctcg acgatcatcc ctatccgttt
    16081 aaagtgaatc cgcaattcaa agcgtgggtg ccggtaactc aggtgccaaa ctgctggctg
    16141 ttggtggatg gcgtgaacaa gccgaaactg tggttttatc tgccggttga ttactggcac
    16201 aacgtcgaac cgctgccgac ctccttctgg actgaagatg tagaagtgat cgcgctgccg
    16261 aaagccgatg gcattggtag cctgctgcct gctgcgcgcg ggaatatcgg ttatatcggt
    16321 ccggtgccgg agcgtgcgct gcaactgggt attgaggcca gcaatatcaa cccgaaaggg
    16381 gtgatcgact acctgcatta ctaccgctcc ttcaaaaccg agtacgaact ggcctgtatg
    16441 cgtgaagcgc agaaaatggc ggtcaacggt catcgcgcgg cagaagaagc gttccgttct
    16501 ggcatgagcg agttcgatat caatattgcc tatctgaccg cgaccggtca tcgtgatacc
    16561 gacgtacctt acagcaacat tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc
    16621 aaactggacc accaggcacc ggaagagatg cgcagcttcc tgctggatgc cggggccgaa
    16681 tataacggct atgcggctga cctgacccgt acctggtcgg caaaaagcga taacgactac
    16741 gcacagctgg tgaaagacgt aaatgatgaa caactggcgc tgatcgccac catgaaagca
    16801 ggcgtcagct atgtggatta ccacatccag ttccatcagc gcatcgccaa actgctgcgt
    16861 aaacatcaaa tcatcaccga tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga
    16921 ccgtttatgc cgcacggtat cggtcatccg ctgggcctgc aggtgcatga cgtcgccggt
    16981 tttatgcagg atgatagcgg tacacacctc gcggcaccgg caaaatatcc gtacctgcgc
    17041 tgcacccgta ttctccagcc gggcatggtg ttaaccatcg agccgggtat ctacttcatc
    17101 gaatcgctac tggcaccgtg gcgtgaaggg cagttcagta aacactttaa ctggcagaaa
    17161 attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag acaacgtggt gatccacgaa
    17221 aacaacgtgg aaaacatgac ccgggatctg aaactggcgt gatggaaagc tggttaattc
    17281 ctgcggcacc agtcacggtc gttgaagaga tcaaaaagag ccgcttcata acgatgttgg
    17341 cgcataccga tggcgttgag gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc
    17401 ccgatgcccg ccaccattgc gtggcgtggg tggcgggtgc gccggatgat tctcaacagc
    17461 tcggtttctc tgacgacggg gaaccggcgg gaacggccgg taaaccgatg ctcgcccaac
    17521 taatgggcag cggcgtcggg gaaattaccg ctgtggtggt gcgctactac ggcggcatat
    17581 tgcttggtac cggtgggtta gtgaaagcgt atggcggcgg agtgaatcag gcgctgcgcc
    17641 agctaacgac ccaacgcaag acgccattaa ccgaatatac tttgcaatgt gaatatagtc
    17701 agttaaccgg cattgaagcg ttgctggggc agtgtgacgg caaaattatc aacagtgatt
    17761 atcaggcatt cgttctgctg cgggtggcgc ttccggcggc gaaagtggct gaattttccg
    17821 caaaactggc ggattttagc cgtggttcat tgcaattgtt agcgattgaa gaataatccc
    17881 cacttcgttt tgcagaacta aggaagcggc agagatgcat tttcgcgcca ttacccgaat
    17941 cgttggacta ctggtcatct tattttcagg gaccatgatt atccctgggc tggtagcact
    18001 catctaccgg gatggagcgg gccgcgcttt tacccagacc ttttttgtcg ccctcgccat
    18061 tggctctatg ctgtggtggc cgaaccgtaa agagaagggc gaactgaaat cccgtgaggg
    18121 gtttctgata gtggtgctgt tctggaccgt gctgggtagt gtcggtgcgc tcccttttat
    18181 cttctcggaa agcccgaacc tcacgattac cgatgcgttt tttgaatctt tctctggcct
    18241 gaccaccacg ggggccacta cgctggtggg gctggattcg ctccctcacg ccatcctatt
    18301 ttatcgccag atgctgcaat ggtttggcgg gatggggatc atcgtgttgg cggttgcgat
    18361 actgcctatc ctcggcgtgg gtgggatgca gctctatcgc gcagaaatgc ccggcccgct
    18421 gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg aaaaccctgt ggttgattta
    18481 tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt gccggaatgg atgcctttga
    18541 tgccatcggc catagctttg cgactatcgc tattggcggc ttctcgacac atgatgccag
    18601 tatcggttat ttcgacagcc cgactattaa cactatcatt gctatcttcc tgctgatctc
    18661 cggctgtaac tacggtctgc acttttcgct gttaagtggg cgtagtctga aggtttattg
    18721 gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt acgctggtgg ttatttgtac
    18781 cctcgtactg tggtttcata atgtctacag ttcggcgctg atgacaatta accaggcgtt
    18841 tttccaggtg gtgtcgatgg cgacaaccgc cgggtttacg actgacagca ttgctcgctg
    18901 gccgctcttt ttaccggtac tgcttttatg ttcagctttt atcggcggtt gtgccgggtc
    18961 aacgggcggt ggcctgaaag tgatccgcat cctgctgctg tttaagcagg ggaaccgtga
    19021 gctgaaacga ctggtgcatc cgaacgccgt ctatagcatt aagctgggga atcgcgcact
    19081 gccggaacgt atcctcgaag ccgtttgggg atttttctcc gcctatgcat tggtgtttat
    19141 tgtcagtatg ctggcgatta tcgccacggg cgtggatgac ttttctgcct ttgcctcagt
    19201 tgttgcgaca ttgaataacc tggggccagg gcttggcgtg gttgctgata actttaccag
    19261 tatgaacccg gtggctaaat ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt
    19321 ctttacattg ctggtgctct ttaccccgac tttctggcgt gaatgatgga gtaatacgtg
    19381 aaaacattaa ttcttttctc aacaagggac ggacaaacgc gcgagattgc ctcctacctg
    19441 gcttcggaac tgaaagaact ggggatccag gcggatgtcg ccaatgtgca ccgcattgaa
    19501 gaaccacaat gggaaaacta tgaccgtgtg gtcattggtg cttctattcg ctatggtcac
    19561 taccactctg cgttccagga gtttgtcaaa aagcatgcga cgcggctgaa ttcgatgccg
    19621 agcgcctttt actccgtaaa cctggtggcg cgtaaaccgg agaagcgtac tccacagacc
    19681 aacagctacg cgcggaagtt tctgatgagt tcacaatggc gtcccgatcg ctgtgccgtc
    19741 attgccgggg cgctgcgtta tccacgttac cgctggtacg accgttttat gatcaagcta
    19801 attatgaaga tgtcaggcgg tgaaacggat acgcgtaaag aagttgtcta taccgattgg
    19861 gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa ccgacaaatc gacgctgaaa
    19921 taagcataaa gaataaaaaa tgcgcggtcg gaaaattatt ttaaatttcc tcttgtcagg
    19981 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg
    20041 ggtcagcggg gttctcctga gaattccggc agagaaagca aaaataaatg cttgact
//
LOCUS       DAOTRK010000085        19564 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.085, whole genome shotgun sequence.
ACCESSION   DAOTRK010000085 DAOTRK010000000
VERSION     DAOTRK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.085"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(105..851)
                     /gene="cutC"
                     /locus_tag="QIE41_002921"
     CDS             complement(105..851)
                     /gene="cutC"
                     /locus_tag="QIE41_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDS3531790.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(865..1431)
                     /locus_tag="QIE41_002922"
     CDS             complement(865..1431)
                     /locus_tag="QIE41_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDS3531791.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEKIVASEQSA"
     gene            1647..3380
                     /gene="argS"
                     /locus_tag="QIE41_002923"
     CDS             1647..3380
                     /gene="argS"
                     /locus_tag="QIE41_002923"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDS3531792.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3433..3825)
                     /gene="flhe"
                     /locus_tag="QIE41_002924"
     CDS             complement(3433..3825)
                     /gene="flhe"
                     /locus_tag="QIE41_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDS3531793.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3825..5903)
                     /gene="flhA"
                     /locus_tag="QIE41_002925"
     CDS             complement(3825..5903)
                     /gene="flhA"
                     /locus_tag="QIE41_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDS3531794.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGTGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5896..7044)
                     /gene="flhB"
                     /locus_tag="QIE41_002926"
     CDS             complement(5896..7044)
                     /gene="flhB"
                     /locus_tag="QIE41_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDS3531795.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPQVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7246..7890)
                     /gene="cheZ"
                     /locus_tag="QIE41_002927"
     CDS             complement(7246..7890)
                     /gene="cheZ"
                     /locus_tag="QIE41_002927"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDS3531796.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7901..8290)
                     /gene="cheY"
                     /locus_tag="QIE41_002928"
     CDS             complement(7901..8290)
                     /gene="cheY"
                     /locus_tag="QIE41_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDS3531797.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8305..9354)
                     /gene="cheB"
                     /locus_tag="QIE41_002929"
     CDS             complement(8305..9354)
                     /gene="cheB"
                     /locus_tag="QIE41_002929"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDS3531798.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9357..10217)
                     /gene="cheR"
                     /locus_tag="QIE41_002930"
     CDS             complement(9357..10217)
                     /gene="cheR"
                     /locus_tag="QIE41_002930"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDS3531799.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHQGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10236..11837)
                     /gene="tap"
                     /locus_tag="QIE41_002931"
     CDS             complement(10236..11837)
                     /gene="tap"
                     /locus_tag="QIE41_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDS3531800.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYHISALVFISMIIVAALYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAAATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLHIAPVVS"
     gene            complement(11883..13544)
                     /gene="tar"
                     /locus_tag="QIE41_002932"
     CDS             complement(11883..13544)
                     /gene="tar"
                     /locus_tag="QIE41_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDS3531801.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKMYRDIITDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIASGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13689..14192)
                     /gene="cheW"
                     /locus_tag="QIE41_002933"
     CDS             complement(13689..14192)
                     /gene="cheW"
                     /locus_tag="QIE41_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDS3531802.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14213..16177)
                     /gene="cheA"
                     /locus_tag="QIE41_002934"
     CDS             complement(14213..16177)
                     /gene="cheA"
                     /locus_tag="QIE41_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDS3531803.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRNQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGTDSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPCEADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16182..17108)
                     /gene="motB"
                     /locus_tag="QIE41_002935"
     CDS             complement(16182..17108)
                     /gene="motB"
                     /locus_tag="QIE41_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDS3531804.1"
                     /translation="MKNQAHPIIVVKRRKAKGHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEEPEVAPQVSVPTMPSAEPR"
     gene            complement(17105..17992)
                     /gene="motA"
                     /locus_tag="QIE41_002936"
     CDS             complement(17105..17992)
                     /gene="motA"
                     /locus_tag="QIE41_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDS3531805.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18119..18697)
                     /gene="flhC"
                     /locus_tag="QIE41_002937"
     CDS             complement(18119..18697)
                     /gene="flhC"
                     /locus_tag="QIE41_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDS3531806.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEDGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18700..19050)
                     /gene="flhD"
                     /locus_tag="QIE41_002938"
     CDS             complement(18700..19050)
                     /gene="flhD"
                     /locus_tag="QIE41_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDS3531807.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4649 a         5437 c         4801 g         4677 t
ORIGIN      
        1 ttcatttttt atactattca gggataaaaa aaggcccctg ttgaaattgc aggggcctgg
       61 tacgagcaag catcatattg ggcgacatga tgcaacggta aaaatcattt ggcctgatgg
      121 cgttcaatga ttcctttcat ttcagcaacc gccgccccgt ctacgacata acgcgaatac
      181 tcgtccgcgt gtgcatctga tgacatggac aatccttgat tacgataacg cattggtgag
      241 gcttgccacg ctcccgcgga gctatggact tccagcactc cggcatcgag gaagtggtgc
      301 aggttttctg cacggactcc tgctccggcc ataatgattg gagcatcacc atgggcaata
      361 agttccataa tttttgataa accttgcagt gcgtctgatt tttgccctga tgtcagtacc
      421 cgagcaacgc ccaattccgc aagattattg agtgtattta aagggttagc gcacatatcg
      481 aaggcgcgat gaaaagtcac tgccagcgga ccggcagcag ccattatttt ttccattcgt
      541 ggcatatcga cattcccgtc aacatcgaga acgcccgtca ccagtccagg aaaacctaat
      601 tcgcggactg tgcgcacatc ctcaagaatg gcggcaaatt caccgtcgct gtagcaaaaa
      661 tcaccaccgc gtgggcggat aatcggatgc acagggatcg tcacccgctg gcgtacggat
      721 ttcagtaccc ccaacgacgg cgttaagccc ccctcttttg gggctgcgca taattcaact
      781 ctgtctgcgc cgttttgctg cgccgttagt gcacattcca tgctgtaaca gcaaatttcc
      841 agtaatgcca tttttactcc ttaattacgc cgactgctcg ctggcaacga tcttttcaat
      901 ggaccacgga tgaaacttaa tagttgtttt gccatcggtg accgccagcg tcggattcgg
      961 taatcgctca tgctcacctt ttggcgaact ggttttcact gagatccccg gcaggctgag
     1021 tccttcatca gaaagcaatg ccagcgcacg ggcattcagc gtttctggat cgcccggcag
     1081 aacgatttca atatgttccc aaccttcgtg cgggtaacgt ttttccccgg gccacggtag
     1141 ctccacaatg gaaaactgcc agtgcgcaac ctgtaccggt tcatgcaatt taaacagaca
     1201 aatcggcctg ccattgatca tattttctga caaaagctcg ccgcactgtt caaacccgcg
     1261 acgccagcgc tcagcggtgg cgttttgatg gcaacgcaaa gaaatgtgat cggcagtcag
     1321 cggagtgata tccaacccca aacggcggga aagttcatct aatgcgtggg taaatcgcgg
     1381 taaatccgat gcaatatcct gcagctcgtc gatagattgc cagttcgcca taatcactct
     1441 tcgtctttca gtaaaagcgt taatttaccc tgttgccctg cgccaaccaa ccgctgattt
     1501 cacgccgctt ctgatgcaat agtgaaaacg gcaatacgcc acgcgcacgt tgctgacgaa
     1561 aacagccatt tgcagtatac tcccgcccta atttctttaa ctggtgcggg caatttttgc
     1621 tcgcttcatc aatgtaaggt attccggtga atattcaggc tcttctctca gaaaaagtcc
     1681 gtcaggccat gattgcggca ggcgcgcctg cggattgtga accgcaggtt cgtcagtcag
     1741 caaaagttca gttcggcgac tatcaggcta acggcatgat ggcagttgct aaaaaactgg
     1801 gtatggcacc gcgacaatta gcagagcagg tgctgactca tctggatctt aacggtatcg
     1861 ccagcaaagt tgagatcgcc ggtccaggct ttatcaacat ttttcttgat ccagccttcc
     1921 tggctgatca tgttcagcag gcgctggcgt ccgatcgtct cggtgttgct acgccagaaa
     1981 aacagaccat tgtggttgac tactccgctc cgaacgtggc gaaagagatg cacgtcggtc
     2041 acctgcgctc taccattatt ggtgacgcgg cagtgcgtac tctggagttc ctcggtcaca
     2101 aagtgattcg cgcgaaccac gtcggcgact ggggtactca gttcggtatg ctgattgcgt
     2161 ggttggaaaa gcagcagcaa gaaaacgccg gtgaaatgga gctggctgac cttgaaggtt
     2221 tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag cgtgcacgta
     2281 actacgtagt caaactgcaa agcggtgacg aatatttccg cgagatgtgg cgcaaactgg
     2341 tcgacatcac catgacgcag aaccagatca cctacgatcg tctgaacgtg acgttgaccc
     2401 gcgatgacgt catgggtgaa agtctctaca acccaatgct gccgggtatc gtggcggacc
     2461 tgaaagccaa aggtctggca gtagaaagtg aaggcgcgac cgtcgtattc cttgatgagt
     2521 tcaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat ggtggctatc
     2581 tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg catgccgatc
     2641 gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggcg tgggctatcg
     2701 tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg ttcggcatga
     2761 tgctgggcaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg aaactggcgg
     2821 atctgctgga tgaagccctg gagcgtgcac gtcgtctggt ggcagaaaag aacccggata
     2881 tgccagccga cgagctggaa aaactggcta acgcagttgg tattggtgcg gtgaaatatg
     2941 cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac atgctggcgt
     3001 ttgagggtaa taccgcgcca tacatgcagt atgcatacac gcgtgtattg tccgtgttcc
     3061 gtaaagcaga aattgacgaa gagcaactgg ctgcagctcc ggttatcatc cgtgaagatc
     3121 gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc gtggttgccc
     3181 gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt ctgttctctg
     3241 gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt aacagccgtc
     3301 tgaaactggc acaactgacg gcgaagacgc tgaaactggg tctggatacg ctgggtattg
     3361 agacagtaga gcgtatgtaa tcgcgttata cggcaaaagc cgatgtcatg gcagacatcg
     3421 gcttttttat cattaacgat aattcacaat cacttcattc cgttgtacct ttagcggcgg
     3481 aattaaccgc ccaccgccag gcacttccca gataaatcgc aacggttcta tcgccggtat
     3541 accggaaaag gccacggtgg ttccactctg cccttctaat tcgacacaac gagattgtga
     3601 acacaagcga acccgcagtc ctgcaggtgt cgggccgata agctgataac gccacgccac
     3661 cagcgtcatc aatcctgcag caggttgtcg agtagaaaga ggcgctgacg acatcgactc
     3721 accacggtga ttcagcgtaa cgcccacact actcgcctgc cacatcccct ccccggcggc
     3781 ttgcaccagc agcggaaaca ataatattgc taataaggtt ctcatcattt accgccaatt
     3841 gtcgccgtca tgcggatatg tcggttatca gatagttcca gattcgacag caccactaac
     3901 tgcggcaagc tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg gttcaccaat
     3961 aaaactggcg gcgcacccaa catctcctga cgggatagag cttcctgagt ttgcgccagt
     4021 aaacgatccg ccagccctgg ctccagtccg cccccgccct gcaacgcctg tagcaacaaa
     4081 cgttccagcg gtgtatcgag gccaataaca tgaacttcat ctttgccagg aaaccactgc
     4141 tgggtaatcg cccgtcccaa cgccacgcgc acgacggcgg ttaattcatg tggatcgctt
     4201 tggatgggcg catgttccgc cagcgtttcg agaatagtgc gcatatcgcg aatcggcact
     4261 ttttcatcga gcagattttg cagcacttta tgcagtgtgg tgagcgtgac gacgccagga
     4321 acgagatctt ccgtcagctt tggcatctcc tgggcgacgc gatccaacag ctgttgcgcc
     4381 tcctgacgac caaacagctc tgcggcatgc tggctaatga ggtggttaag atgcgttgcc
     4441 accaccgtgc tggcctcaac cacggtgtac ccctgaatct gcgcctgttc tttaagcgca
     4501 ctttcaatcc agatagcatt caggccaaat gccggatcga cggtcgcctc accaggtaac
     4561 gtcccggcag cggtacctgg gttaattgcc agccagcgcc ccggataggc atcgccagtg
     4621 ccaatctcta cgcctttcat caaaatgcga taacgtgcag gttgcagatc catattgtcg
     4681 cgaatgtgca ccaccggcgg cagaaacccc atctcctgag caaatttctt acgaatacta
     4741 cgaatacggc ccagcaactc gccatcttgt tggaaatcaa ccatcgggat cagtcgataa
     4801 cccacttcca ttcccaagga atcttccagt tgtacatcgt tccacgtcgc ttcgacaacg
     4861 gtattattct ctgccatttt taccggtttt ggttcggcag gcgctttttg ttcacgtccg
     4921 cggatccacc aggccagccc aagcaatccg gcagtgaaca gcaaaaatac caggttcggc
     4981 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgctcggg
     5041 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5101 acgatgaccc ccgcggcggt agaaatcacc agcgccggga tttgtgccac cagaccgtcg
     5161 ccaatggtca atagcgtata actttccgcg gcgtgtccca tgctcatgcc atgttgcagc
     5221 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     5281 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     5341 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccggcgtt aaggtcggcg
     5401 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcccc cacttctgcg
     5461 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacgaacacc
     5521 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     5581 ccagccgccg ccgcgccggt atgcccttcc atcaaaatga tgcgggttga agctacgtta
     5641 agtgccagac gcaacaacgt agtaaacaat agaatggtcg gaaacgcagc aaactcaagc
     5701 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     5761 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     5821 atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc aggcaggcgc
     5881 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     5941 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6001 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6061 accgatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtagggac
     6121 gttattttca gcgccaattt cacgaatgcg taatgccacc agccccgcgc ccttggcaac
     6181 gacttttggc gcactcattt tgttttcgtc gtactgcaaa gccactgagt agtgagttgg
     6241 gttattgacg ataacgtccg ctttcggcac atcggccatc atccgacgcc gtgcggccgc
     6301 tcgctgcatc tgacggatcc gccctttaac ttgggggtcg ccttcgcttt gtttgaactc
     6361 atcacgaata tcctgccgcg acattcgcag ctttttcagg tggctgaaga tttggaaaaa
     6421 gacgtcaaat cccaccatcg gaataacacc aagtaccacc agcagcgcgc atagccctac
     6481 cagatccatc gcattaccca tggcggtaat cggagactcg gccattaagc gcatcatctg
     6541 cggccagtga tgccagagaa aaaatcccgt cacgctgcca accaggatgg ttttcaaaat
     6601 tgccttaagc aactccgcgc cagtctgagc cgagaatatc cgtttaatgc ccggtagcgg
     6661 gttgagtttg gaaaacttcg gctgcaagga ttttccgcta aataccagcc ctcccagcat
     6721 gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca gcgccagcat
     6781 ggcttctctg atcagcagaa taatttgccc gaggatcaga ttcgggtcat tgataatact
     6841 gtgatcaaaa tgcagcccag cggagagcat gcccgacaat cgacgggcca gcgacacacc
     6901 gccaaaccag ataacactaa cgcccactaa caaaatcagc agtgaagtca gttcacggga
     6961 acgcgggatt tgcccctctt cccgcgcttt ttctagtcgg tgaggtgtgg gggcttctgt
     7021 tttgtcgtcg ctctcgtcag acacgtcgcc aatcctggaa agagtattaa gcgtaaatga
     7081 tgccagagcg caaagcgttc aatggtttga gtaaggggca aaacaggcgg gatttagggc
     7141 ttttactgcc acacatcaag catagtatgc gtttgtcgga tgcggcgtca tcgcgttata
     7201 cgaccgcctg atatgacgta gtcacgccac atcaggcaat acaaatcaaa atccaagact
     7261 atccaacaaa tcgtccacct gatcctgact ggctaccaca ccggctttgc tggtatcgac
     7321 ctgaggtcca ttaagcaaac tctggttttc acgttttgga cgcgactcct gttcggggat
     7381 gttttccaac agcaccatca gcaactgacg ttcgatctcc tgaatgacat ccatcatccg
     7441 cttaatgacc tgtccggtga gatcctgaaa atcctgcgcc atcatgattt ccagcagctg
     7501 cgcgttagta aagctggtat gcgcggggac atctgccaga aattgccgcg tatctgttac
     7561 cagttctcgg gcgtcggcaa ggtcaatcgg atcggcaaac cagtcatccc aacgttgggt
     7621 taacgctttt gctgatttct ccatttgatc ctgatgcggt tgtgacgcct caacactgtt
     7681 cagcgcccgc tccgcagcct gggcggtcat ctgcacaaca tagtacaaac gatcgcgcgc
     7741 atcggggatg gcttccgccg cttcggcaat ggcctgatcc agccccagct cccgcaaact
     7801 gtcgcgcagc atacgcgtca ggctgccgat gcgcgcaatg atatcgccag ctgaatgctc
     7861 gtcagcaggt ttgattgatg gttgcatcat agtcgcatcc tcacatgccc agtttctcaa
     7921 agattttgtt gagtttttcc tccagcgtcg cggcggtaaa tggcttcacc acatagccac
     7981 tggcccccgc ttgcgccgca gcaataatgt tctctttctt cgcttctgca gtcaccatta
     8041 acactggcaa tgctgacatc gcaccatccg cacgaatcgt tttcagcaat tccaggccat
     8101 ccatgttggg catgttccag tcggagataa caaatccata accgcctgcc tgcaacttat
     8161 tgagagcgtc gacaccatct tccgcttcct caacattatt gaatcctagc tctttcagca
     8221 ggttacgcac tatgcgtcgc atggtggaaa agtcatccac aaccaaaaat ttaagttctt
     8281 tatccgccat ttcacactcc tgatttaaat acgtatcgcc tgtccggcac taatttttgc
     8341 caacatttgc tggcttacct ggctaagatc gaccacttcg cagacaccac ccatattgat
     8401 ggcctcgcgc ggcatgccga acaccacgca acttgcttcg ttttgcgcaa gggtccatgc
     8461 ccccgcctga cgcatcgcca acattcccgc cgcgccgtcg ttgcccatac cggtcaggat
     8521 caccccaacc gcattacgcc ccgcctgttt ggcgacagaa tggaacaaca catctaccga
     8581 aggccgatga cggttaaccg ccgggccgtc gtgaattttg atttgataat ttgcgccact
     8641 acgcgccagc tccatatgcc gatcgcccgg cgcaatatag gcatgccccg gcaaaacacg
     8701 ttctccgtct tcggcttctt taaccccgat ctggcaaagc ttattaagtc tgtcggcaaa
     8761 agagcgggtg aaaccgggcg gcatatgctg ggtaattaac agtgccgggc tggaaagcgg
     8821 caacggttgc agtacgtgac gaattgcctc agttccaccc gttgaagcac caatcgcaat
     8881 cagtttttca gaactcaaca acggccctgc cttcagcgtt gtcggtgccg acaatggctt
     8941 atgtgctgca aggctcgcct ttgctgctgt acgcaccttt tcagcaatca tttcgctata
     9001 cgccagcatt ccttcgcgaa tacccagttg cggtttggtg acaaaatcta tcgcccccag
     9061 ctccagcgcg cgcagcgtga cttctgaccc tttgccggtc agggaagaaa ccatcactac
     9121 gggcattgga cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca tccgcggcat
     9181 ttcaacatcc agcgtcaata catcgggatt gaatttctta atcaaatcac gcgcaaccag
     9241 cggatcaggg gcggtcgcca ccatttccat gtcgctatgg ctgttgatga tttctgtcat
     9301 gatctggcgc atcagtgccg aatcatcgac agataacacc ctgattttgc tcatcgttaa
     9361 tccttactta gcgcatacac cgtctgacca cgcagcgtga agcggcgctc aaggtggcta
     9421 aagttttcag agtgacccgc aaacagcaat ccgtcgggtt taaggagcgg aacaaagcgg
     9481 cgcaaaatct cctgctgggt agtttgatcg aagtagatca tgacgttacg acagaagatc
     9541 gcatcaaacg gccccggcac ggtgtactgt ttcgccagta aattcagcgg ggcaaaatca
     9601 acataattcg ccaattcctg acgcacgcgt accagccctt gatgcggtcc cgtccctcgc
     9661 atgaagtacc gctgcagttg ctgcggcgtc agatttttca actcttcatg gcgataaata
     9721 ccgcttctgg ctttttccag cacttcggtg tcaatgtcgc tggcaaacac tttccagcgt
     9781 ccgggcgcgg tgcccaatgt gtcagccagt gtcatcgcaa tgctgtacgg ctcttcgccg
     9841 gtcgaagccg ccgcgctcca tacgcgatac tcgccagaac gacgccgggc gtgatccgcg
     9901 agcagaggaa aatgatgcgc ctcacggaaa aatgccgtca gattcgtggt cagcgaattg
     9961 ataaacgcct gccactcacc gctgtgctga ttagattcca gcaagttcag gtaatggccg
    10021 aaatccgtca gtcccagcga acgcaaacga cgaaccagtc ggttgtaaac catgtcgcgt
    10081 ttatggtcgg ccaggacgat cccggctcgt tgatagatca attgacttat ccgccgaaaa
    10141 tgcgcgtcgg aaagcgccag gcgctcggtc atctgtaaca ataaagacgt ttgcccacag
    10201 ggcagagatg aagtcatagc gccttctcaa tcacttcagg ataccactgg cgcaatatgt
    10261 aactgcgccg actcatgtcg tgctacttca tgttcttcaa gggtaaatac cgccacacgc
    10321 gacgaaagat ggtcagcctg gttcgccagt tgttccgttg ccgccgccgc ttcttctacc
    10381 agcgaggcgt tctgctgtgt cacctgatcc atctggctga cagcctgcgc aacctgctca
    10441 atcccccgcc gttgttcttc cgaggccgaa gcaatttctc ccataatgtc gttcacgcgg
    10501 gtcaccgaac tgacaatatc agtcatggtc gcggcggcgt tattcaccag tttcgaaccc
    10561 tgctggacac gattgactga ctcttcgatc agccctttga tctcttttgc tgcctgggcg
    10621 ctacggctgg caagattgcg cacttcacct gccactaccg caaaaccacg accctgctct
    10681 ccggcgcgag ccgcttccac tgccgcattc agggccagaa tattggtctg gaaagcaatg
    10741 ccgtcgataa cgctgataat gtcgccaatt ttttgcgagc tggtggcgat ctcctgcatg
    10801 gtgtgagtca tggtactgac ctgaacaccg cccgcctgcg ccgttgtcgc ggcatttttt
    10861 gccagttccg acgcctgtcg tgcgttatcg gcgttttgcc ctaccgtggc ggttaattgc
    10921 tccatactgg cggccgtttg tgccagcgat gccgcctgct gttcggtacg acttgagaga
    10981 tcgttattgc ctgcgacaat ctccgcgata ccaatgtgca tctcctggct tcccttacgc
    11041 acatcactta ccgtcccacg caaagcctgc tgcatggtct tcagactggc aaaaatggcg
    11101 gtgatctcat tgcgaccata cactgcaatc ggacgcgcca gattacccgc agcaatgctg
    11161 tcaaaatggc taccgatgat ggccagtggt tgaacaatca tcttgcgcgt ccaccacagc
    11221 gcactgctga tgtagagcgc tgcaacaata atcatgctga taaacaccag cgccgaaatg
    11281 tgatagttac gctggctttg cgcactggca gcttccagca catggttgat ctccagctgc
    11341 caggcctcaa agttaacgtc aaacgcattc tgtgattcct gcaccggcgc agtgaggaaa
    11401 tccgaaagtt gattactttc aagccaggtc gcctgatgtt cgagatcgtt gtgccagcgg
    11461 gcaaaacttt tctccgtctc tttttgcaac gccctgacgt gctcgttgcc cgcagtcatc
    11521 gccataaaac ttttaaacag cgtggtggat tgcgtcagac tggcgcgcgc cgtcgtcatc
    11581 aacgttttaa tgtcatccgc cggatagcta agcgccgtca gagtgcccgc tttgttcagc
    11641 gcggtactgg cctgtaacat taccgcccga gtttgcgcta aagccgcacg ttgctgattg
    11701 ctctgttcga cctgattcag tcgttgcaaa tcgtcgcgaa atgcccaaaa agacatgccg
    11761 ttactgccaa tctgcaagat cccgcagaga atcaaaatta aaaacagcgt ggtcgaaatt
    11821 cgaatacgat taaacatcaa cgctccccat caggcggcaa tgaccgcgtt agtaaatact
    11881 cgtcaaaatg tttcccagtt tggatcttgt tcagcaattc gcagtcgtgg ctgtgccggt
    11941 ggttgctcac tggcaggacg ggatggtgtt tgcggtttat tggtgagtgg gctggctgcc
    12001 agacgaaacg cggaaaccgc ttgcgttaaa cgactggcct gttcttccag cgcagctgcg
    12061 gcggcagctg attcctgcac cagcgatgcg ttctgttgcg tgacgcgatc catttccgaa
    12121 accgccaatg cgacttgatc gatgccacgg ctctgttcat ccgatgccga tgcaatctcg
    12181 cccataatgt cagtcacgcg agtgacagca ttgacgatat tgttcattgt ttccccggcg
    12241 ctttcgacca gcaccgaacc ggtatcaacg cgcgagacgg agtcttcaat gagggctttg
    12301 atctcttttg ccgcctgggc gctgcggctg gcaagattac gcacttcgcc cgccaccacg
    12361 gcaaaaccac ggccttgttc acccgctcgt gctgcttcaa cagcggcatt cagcgcgagg
    12421 atattagtct ggaaggcaat accatcgata acgctgataa tgtcggcaat tttcttcgaa
    12481 ctatcggcga tctcatgcat cgttttcact acgccatcca ctactttgcc gccgtgctgg
    12541 gcagtgtcag aggcactttg cgccagttgt gaggcctggc gggcgttatc ggcgttttgc
    12601 ttcactgtcg cggtgagctg ctccatgctg gcggcagttt cttccagcgc agatgcctgc
    12661 tgttcagtac gggaggaaag atcggtgttg cctgccgcaa tttcacgggt accggcatag
    12721 atggcatctg aaccttcacg cacatgagtg acggtgtcag tcaaagagcg ttgcatatgt
    12781 gaaacgctct gcgccaggtc gcccatttca ctgcgcccgt caatggtcag ggtattcgcc
    12841 aggttaccac tggcgatttc gcgaatgtga gcaataattt ttgccagcgg tgtaagcaac
    12901 atacggcgaa tgccgtacca cgccaccagc agaatcaata ccaccaccag cgcaataacc
    12961 gccagttgcc attgggcaaa tcggtaatca tctgcgttgt cagtgatgat atcgcgatac
    13021 attttttcac tgctgagggc gtactgagca aacgcttcgc ccattgcatt ttgcattccc
    13081 tgggttggct gagcgaaata agctccagta ttgccataat caagataatc aatcagttca
    13141 gttaacgctg tgtgatagtt tttatatttt tcatcaatat tacgactggt agcgaccatt
    13201 tcaggtaacg gtgccatgct tttgaatttt ttataatgcg tcgctgcctg ggccaatgtt
    13261 ttcctggcgc tatcgagcaa ttcaactttg gcgttacttt gttgattgga ggaatccatc
    13321 atcatccgca ccgctgaacg actcaggtta atgcgcgttt gcagcattaa atcccaggtt
    13381 gacgtcagct cgccctgctg ttcccgtaat tgattggaaa ccacaaagct cttctggcta
    13441 tggtgaaggg aagaaaaaaa cagactgccg gaaataagct gtaacagtgc gaataccccc
    13501 agcaccatta ccaacagcgt gactacgcgg atacggttaa tcataaggca ccttcctgaa
    13561 aacaagttga tctcgttatc ggcaaggaag ggggaaactt tattgctgat gccacccgcc
    13621 gcgaaactga aataaaaaac ccgatgcgca ggtcatcggg ttcattgcaa ttgaggaaat
    13681 cgggagaatt acgccacttc tgacgccgcg ctatctaaca gcgccatctc ttcgctgttc
    13741 agcagttttt cgatgttcac cagaatcaac atccggtcgc ccagtgcgcc cagtccagtg
    13801 agatattctg ttgaaagcgt cacggcaaat tccggtgccg gacgaatttg ctccgccgtc
    13861 aatgaaagca cgtctgaaac gccgtcaacc acgatgccga ccacccgctg tccgagattc
    13921 aggacgataa ctaccgtgtt gtcgttatag tccacatcca cctggctgaa cttaattcgt
    13981 aagtccacaa tcggcacaat aacaccgcgc agattcgtga cgcctttgat aaacgctggc
    14041 gtgttcgcaa tccgtgttac ctgatcatag ccacggatct cctgcacttt caggatatca
    14101 ataccgtact cttcatcacc aagggtaaat accagaaatt cctggcctga cggctcgctg
    14161 gccagctttg ttacattcgt cataccggtc atattgttac ctttttactc attcaggcgg
    14221 cggtgttcgc catacgttgt tcgcggttta tcgcctgcaa ggcggagaca tcaacaatca
    14281 gtgccacgct gccgtcgcca agaatggtcg cagcagaaat gccggggact ttgcgatagt
    14341 tactttccag gtttttaacc acaacctggt gttgaccaat taattgatcc accagcaagg
    14401 cgtagcggcg accgccactt tgtaagatca ccacaattcc ctgggtggct tcggttttcg
    14461 cgcccgcgac gttgaacact ttccacagtt cgacgatagg cagatattca ccccgcactt
    14521 ccagcacccg ctcgccgcca gccagtggat gcagatcggc ttcacagggt tgcagtgatt
    14581 ccatgacagc attcagcggc agaatgaaaa cttcatccgc aacgcgtacg gacatgccgt
    14641 cgaggatggc cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagta ccctgcttcg
    14701 actggatttc gacatgaccg cccatctcct ggatattacg tttaacgacg tccatgccga
    14761 cgccgcgccc ggagacgtcg gtgacctgct ctgccgtgga gaagccaggt gcaaatatca
    14821 gcattgcgac ttcgtcgtcg ctcatgtttt cgctgacggt cagaccttgc gaggccgctt
    14881 ttgccagaat tcgctcacgg tttagccccg cgccatcgtc ggtcacttca atgcaaatgt
    14941 tgccgccctg atgttcggca gaaagaatta aatttccgac gctattttta cctgcggcga
    15001 gccgtttttc tggcagttca ataccgtgat cgaggctatt gcgtaccagg tgggtcagcg
    15061 ggtcgataat gcgttctatc aggctcttgt cgagttcagt ggaactgccc accagcgtca
    15121 gttctacctg cttgccgagt tttcccgcca gatcacgcac cagccgggga tagcgactaa
    15181 agacatattc catcggcatc atgcgaatcg acatcactga ttcctgcaaa tcacgagcgt
    15241 tacgttgtaa ctgccccatg ctggtaatca aatcaccatg attaaccggg tccagttcac
    15301 tggagcgctg ggcaagcatg gactgggtga tgaccagctc gccgacgagg ttaattaatt
    15361 gatcaacctt ttctaccgct acacggatgc tggtggattc actactgcgc gtcgtttttt
    15421 cccgctccac gcgaccggtt ggcgcttgtt cggctgccag tttaagcact ggtggggggg
    15481 atattttggg cgagacttct actgtttcaa aggtaatctg atcggcttca atcacaaaac
    15541 agagtaccgc tgtgatgtca tcttcggcga tgtcgcccgg taatattgcc gagagagaat
    15601 ccgtcccttt caccacgtca gttaacgttg tcagatgccc cagctcttct tccagcaggt
    15661 cgacttcccc ggccttcagg cgcgaaagga taattcgtcg cggcaactga ttgcggctct
    15721 gctcatcttg cggttcactt ttggcaacca cacttaatcg ggtcactgcg gatggcgttt
    15781 cgcctttcgc ttctaatgcc agttgacgca aggcctggca gatataatcg aagctggcag
    15841 catccggctc ttgcgactgt ttataagcgt cgagctgttc ttgcatgatg tccttcgttt
    15901 ccaaaaacag attgataatg tcggtgttga gttgcatctc acctcgtctg gcttcgtcga
    15961 gcaggttttc catcagatgc gtggtttcct gcaaaacgct gaagccaaag gttcctgccc
    16021 ctcctttgat cgagtgggca gcccgaaaga tggcattcaa ttgttcggca tctggcgctt
    16081 ccggctgcaa aaccagcaga tgctgctcca tgtcagccaa cagttcgtcc gcttcatcaa
    16141 aaaatgtctg ataaaaatcg cttatatcca tgctcacgct gtcacctcgg ttcggctgat
    16201 ggcattgtgg gaacactgac ctgtggtgca acctcaggtt cttccagggc gcttactggc
    16261 tcattctggc tttcggcgtt ttcatgcaaa atggcctgtt cggcttgttt gttcagtacc
    16321 agcaggctga tgcgacggtt gacagcatca tcaggaccgc gatcgcttaa gcgcatcgtt
    16381 gccgccatgc cgacgacacg taacactttg ccgccattca acccgccaac catcagttcg
    16441 cggcgggatg cattggcccg atcggcagaa agctcccagt tgctatatcc tttctcacca
    16501 ctggcgtaag ggaaatcatc ggtatgaccc gaaaggctaa tacggttggg aataccattc
    16561 agaacgggcg cgatggcgcg cagaatgtcg cgcatatagg gttccacatc ggcactgccg
    16621 gttctgaaca tcgggcgatt ctggctatcg atgatctgaa tacgtagacc ttcctgaacc
    16681 agatcgattt tgagatgtgg gcgtaacgcc cgcagtttcg gatcggattc aatcaactgg
    16741 tcgagatcac cccgcaattt ccgcaatcga ctttgctcca tgcgtttttt cagctcttcg
    16801 atgttcggct gcttattcac ttccccctgg ctttgggtgt aatcatcacc cccaccggga
    16861 attgggcttt cactattaga aatgcgatcg ccgcccgtaa ccgcagtcgc cagtggagtc
    16921 cggaagtact ccgcaatctg aatcaactct tttgggctgg agatggagat cagccacatc
    16981 accagaaaaa aagccatcat cgcggtcata aagtcggcat aagcaatctt ccacgatcca
    17041 tgtgctgccc cgtggccttt ggctttgcgt cgtttgacga caataatcgg atgcgcttga
    17101 ttcttcatgc ttcctcggtt gtcgtctgtt gttgcggatt tttcaccgca cgcacatgct
    17161 cttccagttc aataaacgac ggacgttcgc tggaatagag cgttttgcga ccaaactcaa
    17221 cggcgatagg cggtgcgtaa ccgttcagat tagaaagcag tgtgactttg acgcactgca
    17281 tcattttgct ggtttcggcg cttttctgac gtaaaacact cgctaatggg gaaataaatc
    17341 cgtaagccaa taaaataccg aggaaagtcc ccaccatcgc atgtgcgata agcgccccca
    17401 gctcggcggc aggacgatcg gctgaaccta acgcgtgaac gacccccatc acagcagcga
    17461 caataccaaa cgccggaagt gagtccccga ccagcgccag actgttcgcc gggacttctg
    17521 cctcgctttc gtgcgtctca atctcttcat ccatcagggc ttcgatttcg aaggtgttca
    17581 tgtgaccgct gataatcaga cgcagataat cgacgataaa atcaagcatg acgctatccg
    17641 caaggatgcg tgggtagctg gcgaagatct cgctctcacg gggattttca atatcacgtt
    17701 ccagcgaaaa catccccatc tgccgcgatt tcgccatcaa ccgataaagc agagccagca
    17761 gatccatata cattgctttg gtgtatttgg agcgacgaaa cagcaacggc agcgccttca
    17821 gcgtgccttt aatcgctttg ccattattgc cgacgataaa cgacccaatc cctgcaccgg
    17881 caataatcac cagttcagcg ggttgataga gtgctccaag gcttccaccg gtcatcaaat
    17941 aaccgccgaa aactgtaccg agaacaacca ggtaacctaa taagataagc acgacatcat
    18001 ccttccactg ttgaccatga caggatgttc agtcgtcagg cgttaacgcg cgattggggc
    18061 aaaaaaaagc agcggtacgt cgttaccgct gctggaatgt tgcgccacac cgtataagtt
    18121 aaacagcctg tactctctgt tcatccagca gttgtgggat aatatcggca ggattctggg
    18181 aaagtttacg tctttttact gcccgggatg gcggctgaca taagctgcag gcaaagctgc
    18241 caacaggctg gtgagcgtgg gtaataaaat tgccgccgca gcagttgcag ctggaaagtt
    18301 gcagtaatcc actttcaaca aaccgcacca atgtccaggc acgggttaat gccagcagcg
    18361 gtccgtcttc tgcttgcggg cactgttcaa ggtataaacg gtaggctttg atcaccgcat
    18421 cgacgccatt acacaaaccg gttttcagta aaaactgcca tgcgttacag aacatcgaag
    18481 catgaacgtt ttgttcccag gtcataaacc agtcggttga gaatggcagc atgcctttcg
    18541 gcggtgggct tccgcgcagt tctttataga gttttatcag gcgtccgcga cttaactgtg
    18601 tttcgctttc cagcatctgc aaacgagcgc caagggtgat caattccatt gccagctgaa
    18661 tatcccgcgc ttcctgaaca atgctttttt cactcatgat caggcccttt tcttgcgcag
    18721 cgcttcttca ggctgattaa catcattcag caagcgtgtt gagagcatga tgccggtatg
    18781 aatttgctgg agatcgtcaa cgcgggaatc ttgcgtcaac tgagtaatcg tctggtggct
    18841 gtcaaaacga aagtgacaaa ccagttgatt ggtttctgcc agcttaacca tttgcggaag
    18901 agtcagtgcc gctaacgttg tcgccatttc ttcatttatg ccgagacgaa acattgcgga
    18961 cgctttgtcc tgaacaatca gacgctgtgc aagtagtaaa tatgacaagt tgatgtcata
    19021 aatgtgtttc agcaactcgg aggtatgcat tattcccacc cagaataacc aactttattt
    19081 ttatgcggtc tcaccgcacc ccgtgatgtc gccgggaagc cccggtaaaa aataattagc
    19141 attagaatag ttgcgataag ctgcaatagg cagagcccct ttttgggatt taataagtcc
    19201 ttacaaatag aaggggttct ttacacttat ctaagatttt tcctaaatcg acgcaacttt
    19261 actcgtcact acactcacat acaacggagg ggggctgcga ttttcaataa tgtgtgatgc
    19321 agatcacaca aaacgctcaa ttacttaaca taaatgtata agtcatactt ttgttttggg
    19381 tgtatttcaa tctgttaaaa agtttttcgc gacgctagca agcaaaaatg aaacaggaat
    19441 aatcgaaatg ggatgttgcg cacagttaaa ataactcacc gtaaataatc atctgctata
    19501 aataattgct ttaatgcaat accagataag ctatttttaa acagacactt accgcacaac
    19561 aaac
//
LOCUS       DAOTRK010000086        19548 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.086, whole genome shotgun sequence.
ACCESSION   DAOTRK010000086 DAOTRK010000000
VERSION     DAOTRK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.086"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(15..1334)
                     /gene="brnQ"
                     /locus_tag="QIE41_002939"
     CDS             complement(15..1334)
                     /gene="brnQ"
                     /locus_tag="QIE41_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDS3531808.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(1741..3036)
                     /gene="phoR"
                     /locus_tag="QIE41_002940"
     CDS             complement(1741..3036)
                     /gene="phoR"
                     /locus_tag="QIE41_002940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDS3531809.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(3094..3783)
                     /gene="phoB"
                     /locus_tag="QIE41_002941"
     CDS             complement(3094..3783)
                     /gene="phoB"
                     /locus_tag="QIE41_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDS3531810.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            3973..5175
                     /gene="sbcD"
                     /locus_tag="QIE41_002942"
     CDS             3973..5175
                     /gene="sbcD"
                     /locus_tag="QIE41_002942"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDS3531811.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGAQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            5172..8315
                     /gene="sbcC"
                     /locus_tag="QIE41_002943"
     CDS             5172..8315
                     /gene="sbcC"
                     /locus_tag="QIE41_002943"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDS3531812.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAVEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIHYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALQSQQQTLQQQE
                     ALAAQSLQKAQAQFDTALQDSIFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTQQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESMFAVK"
     gene            8441..9625
                     /gene="araJ"
                     /locus_tag="QIE41_002944"
     CDS             8441..9625
                     /gene="araJ"
                     /locus_tag="QIE41_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDS3531813.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     VG"
     gene            complement(9768..10676)
                     /gene="mak"
                     /locus_tag="QIE41_002945"
     CDS             complement(9768..10676)
                     /gene="mak"
                     /locus_tag="QIE41_002945"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDS3531814.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGLRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYHRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            10801..11712
                     /gene="rdgC"
                     /locus_tag="QIE41_002946"
     CDS             10801..11712
                     /gene="rdgC"
                     /locus_tag="QIE41_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDS3531815.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(11790..11873)
                     /locus_tag="QIE41_002947"
     CDS             complement(11790..11873)
                     /locus_tag="QIE41_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531816.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(11870..12208)
                     /locus_tag="QIE41_002948"
                     /pseudo
     CDS             complement(11870..12208)
                     /locus_tag="QIE41_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(12356..12640)
                     /gene="ppnP"
                     /locus_tag="QIE41_002949"
     CDS             complement(12356..12640)
                     /gene="ppnP"
                     /locus_tag="QIE41_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDS3531817.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(12712..13389)
                     /locus_tag="QIE41_002950"
     CDS             complement(12712..13389)
                     /locus_tag="QIE41_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDS3531818.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLEKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(13647..13838)
                     /gene="yaiA"
                     /locus_tag="QIE41_002951"
     CDS             complement(13647..13838)
                     /gene="yaiA"
                     /locus_tag="QIE41_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDS3531819.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(13888..14412)
                     /gene="aroL"
                     /locus_tag="QIE41_002952"
     CDS             complement(13888..14412)
                     /gene="aroL"
                     /locus_tag="QIE41_002952"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDS3531820.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(14595..15053)
                     /locus_tag="QIE41_002953"
     CDS             complement(14595..15053)
                     /locus_tag="QIE41_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDS3531821.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            15173..15982
                     /gene="proC"
                     /locus_tag="QIE41_002954"
     CDS             15173..15982
                     /gene="proC"
                     /locus_tag="QIE41_002954"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDS3531822.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGVMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(15999..17114)
                     /gene="adrA"
                     /locus_tag="QIE41_002955"
                     /gene_synonym="dgcC"
     CDS             complement(15999..17114)
                     /gene="adrA"
                     /locus_tag="QIE41_002955"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDS3531823.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVMLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(17216..17536)
                     /gene="psiF"
                     /locus_tag="QIE41_002956"
     CDS             complement(17216..17536)
                     /gene="psiF"
                     /locus_tag="QIE41_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDS3531824.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(17655..19070)
                     /gene="phoA"
                     /locus_tag="QIE41_002957"
     CDS             complement(17655..19070)
                     /gene="phoA"
                     /locus_tag="QIE41_002957"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDS3531825.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPDNALEKGGRGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(19171..19431)
                     /gene="iraP"
                     /locus_tag="QIE41_002958"
     CDS             complement(19171..19431)
                     /gene="iraP"
                     /locus_tag="QIE41_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDS3531826.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
BASE COUNT         4861 a         5188 c         4917 g         4582 t
ORIGIN      
        1 aaatgttcag tgatttagtg agcgctggag gtcacctgac gacctgccgc gcgatcccag
       61 ataatggcca gaaccaccat caccactgtt ggcattaacc acgccagacc ttgttcggcc
      121 agcggtaaac gctgcgccca ggacggtaag atatcgctga atgcagatgc tttgatcccg
      181 tcgagaatac caaaaagcag gctgataaac atcggcggag caatcacgcg ggacgaatta
      241 tgccaccatg agcgtgtaaa acttaataca accagtgcga tacacggcgg ataaatggcg
      301 gtcagcaccg gtacagagat ctgaatcagc tgactcaagc cgaggttaga caccaccatc
      361 gagaagccgc cgaggataaa caccagcgta cgataagaga gcggtacgta ctgggcgaag
      421 aattctgcac aagcacaggt caggccaacc gccgtgacca ggcaggcgat gaagattaac
      481 gccgccagca ggaagctacc gccgccgcca aaggtatgct gaacgtaagc atgcaggatc
      541 gccgcaccgt ttgcagactg atcgaccagc gacgcgctgt ctgaacccag acggaacagc
      601 gccaggtaca gcagagtcag accaacaccc gccatcaggc cagcccagac ggtataacgg
      661 gtcagcagac gcgcttcggt aacgccacga gaacgcgccg cgttaacaat aacgatacca
      721 aacaccattg cgcccagcgt atccatggtc agatagccgt taacgaagcc gttagaaaac
      781 gcagcgtttt gataagcctc agtcgccgtg ctgatagaac ccgccggcca gacaatcgcg
      841 gcaacagaca ggatgaccag cgcgataatt ttcagcggcg caaggaagtt gcccacggta
      901 tccagcagtt tgcccggata gagcgaaacc agaataacga tagcgaaata aaccaggctg
      961 tagataaaca gcggcagtgc ggaatcaccc gtcagcggcg caatgcccac ttcaaaagaa
     1021 acggtagctg tacgcggcgt agcgaaaagc ggccccaccg ccaggtaaca caccgttgcc
     1081 agcagtacgc cagcgacttt accgattggc gtgctgaggc tgtcaacacc gccgccaact
     1141 tttgccagcg ccactaccgt taataccggc aggccaacgg cagtaatgag gaagccgaat
     1201 gccgcagtcc agacgtgttc gcctgcctgt aagccgacca ttggagggaa aataatgtta
     1261 cctgcgccga cgaacaacgc aaatgtcata aagcccagag cgatgatatc gcgcgatctt
     1321 aattgatggg tcataaatca tactgcctgt ggatgtggtg ttgtaacgtt aaatttttgt
     1381 gccaatccct ggggatgata cagcggtata tttaaacaat tttagcggga aatactccca
     1441 accaggcgcg gcgaagaata gtttttcgat ttaccgcgtg aatgcagtca gtatgacagc
     1501 acctgaagcg caatttaaac gcttataacg ttttaaagca agaagggatc taaaaaccag
     1561 aggtatatag cgataagaaa tcaccaaaca gtataaaaca ctatttccgt gaaaaagaca
     1621 tggctaatca tgcgaacaaa aaatcaccaa ccgttgtaaa atctaacgcc cgtattttga
     1681 ctgcaaagaa aataagccag catcggctgg cttatggcaa taaaagatga caaaggcgga
     1741 ttaatcgctg tttttggcaa ttaaacgttc cgggataaca aaactgaaac gtgttccttt
     1801 tcctactgta ctctcaatat tcaggcgact ttcgtgatga ttcaccgcat gtttcacgat
     1861 cgctaaccct aatccgctac cgccggtttg ccgggaacgc gctttatcaa cgcgataaaa
     1921 acgctcggtc aggcgcggaa tatgctccgg tgcaatgccc ggtccgttat cttcaacgct
     1981 aaattcggca ccgtgcggca ctcgctgcca gcgtacggtg atatgcgtgc cttccggcgt
     2041 atgatttacg gcgttataga ccaggttcga aatcgcactg cgtagctgat cttcgttgcc
     2101 agacaccttg aggccgttat ctatctcaaa ggtaaatgtc tgttttttct gactcagagt
     2161 ctgagcctcg cgctcaacaa cgcgcagcat cattggcaca tcaacctttt cattgagcaa
     2221 atgcgtcggt gcggcttcta ttttcgacag cgtcagcaat tgcttcacca gcccttccat
     2281 ccgctgggtc tgctcgcgca tggtgtgcaa cgctttttca cgtaccgcgc cttccagcgg
     2341 ttgctcatcc atcatctcca ggtaaccctg taacacagtc aatggcgtac gtaactcatg
     2401 gctcacgttg gcaaaaaagt tacgccgcgc cccttccagt tgatgcattt gcgtgacatc
     2461 acgcgccacc atcagcaact gtttgtgggt ataaggcatg acgcgaattt ccagatgccg
     2521 cccggtgttg agcaccagat taagcgggcg agaaaaatca cgcgttttca gatattgcgt
     2581 aaactccggg taacgcagca agttaaggat gttctgcccg ttatcttccg gccagcgcaa
     2641 gccaagaatc tgttgcgcca gaccgttgca ccagaaaata ccgccctctt ccgtggtcag
     2701 caccaccgcg tcgggcagcg actccgcgcc gctacgaaag cgtttaatca gattgcccag
     2761 ttcgcggcgg cgttttttat ttcgcaactg catctggtgt aatccgtata gcagcggttc
     2821 ccagctacca cgtcccggtg gcggggtcat actacgatcc acccacagcc accatgaaag
     2881 gcgcaataaa ttccagaaat gccagataag cagtcctgtt accgatgcca gcaaaaacca
     2941 gggcaggtaa ccaaaaaatg cacccaggat gaaagccggg aggcagcaaa gtagcagctc
     3001 cagcaccagc cttttccacg acagccgttc cagcacgtaa gatactccag ttaagaaatc
     3061 ataagccctg ctctgcgtcc gatgagcaag gcgttaaaag cgggttgaaa aacgatatcc
     3121 tgtaccgcgc acggtctgca ccatgcggtc atgcccgccg ggctccagtg ctttacgcag
     3181 gcgacgaatg tggacatcga ccgtacgatc ttccacatac acgttggttc cccagacgtg
     3241 gtttaacagc tgctcgcggc tgtacacgcg ctcaggatgc gtcataaaaa agtgcagcag
     3301 tttaaattct gtcggcccca tctccagtgg ctcttcgccc gccatcaccc ggtgagatgt
     3361 cgggtcgagg cttagcccct gcatctcgat cacctcttcc accgccattg gcgaaatacg
     3421 gcgcattacc gctttgattc gcgccaccag ctccttcggc gaaaacggct tggtgatata
     3481 gtcatccgcg ccggtttcaa ggccgcgcac gcgatcttct tcttcccctc tggcggtcaa
     3541 catcaccact ggaatatccc gggtcatcga ctcgcgcttg aggtgtttga tgaactggat
     3601 accggagccg ccaggtaaca tccagtcaag gagaattaaa tccggccagg gttcattcag
     3661 ttgattcaca gcactgtcat aatcttccgc ttcgaccggc tgaaagccat tttgttcgag
     3721 cacgaagcag accatttcgc gaattggtgc ttcatcttct acgaccagaa tacgtctcgc
     3781 catgatttgc cctgttgtaa taaataggtt gcgatcatta atgcgacgtc attatgcgtc
     3841 agatttatga cagatttatg aaaagctcgt cgcacatatc ttcaggttat tgatttccgt
     3901 ggcgcagaaa aaagcaaatg gcacatctgt ttgggtataa tcgcgcccat gctttttcgc
     3961 cagggaaccg ttatgcgcat ccttcacacc tcagactggc atctcggcca gaacttctac
     4021 agtaaaagcc gcgaagctga acatcaggct tttcttgact ggctgctgga gacagcacaa
     4081 acccatcagg tggatgcgat tattgttgcc ggtgatgttt tcgataccgg ctcgcccccc
     4141 agttacgccc gcacgttata caaccgtttt gttgtcaatt tacagcaaac tggctgtcat
     4201 ctggtggtac tggcaggaaa ccatgactcg gtcgccacgc tgaatgaatc gcgcgatatc
     4261 atggcgttcc tcaatacgac cgtggtcgcc agcgccggac atgcgccgca aatcttgcct
     4321 cgtcgcgacg ggacgccagg ggcagtgctg tgccccattc cgtttttacg cccacgtgac
     4381 attattacca gccaggcggg gcttaacggt attgaaaaac agcagcattt gctggcggct
     4441 attactgatt attatcaaca acactatgcc gatgcctgca aactgcgcgg cgctcagcct
     4501 ctgcccatca tcgccacggg acatttaaca actgtgggtg ccagtaaaag tgatgccgtg
     4561 cgtgacatct atattggcac gctggacgcg tttccggcac aaaactttcc gccagccgac
     4621 tacatcgcgc ttgggcatat tcaccgcgcg cagattattg gcggcatgga acatgttcgc
     4681 tattgcggct cccccattcc actgagtttt gatgaatgcg gtaagagtaa atatgtccat
     4741 ctggtgacat tttcaaacgg caaattagag agcgtggaaa acctgaacgt accggtaacg
     4801 caacccatgg cggtgctgaa aggcgatctg gcgtcgatta ctgaacagct ggaacagtgg
     4861 cgcgatgtat cacaggagcc accggtctgg ctggatatcg aaatcactac tgatgagtat
     4921 ctgcatgata ttcagcgcaa aatccaggca ttaaccgaat cattgcctgt cgaagtattg
     4981 ctggtacgcc ggagtcgtga acagcgcgag cgagtgttag ccagccaaca gcgtgaaacc
     5041 ctcagcgaac tcagcgtcga agaggtgttc aatcgccgtc tggcactgga agaactggat
     5101 gaatcgcagc agcaacgtct gcagcatctt ttcaccacga cgttgcatac cctcgccgga
     5161 gaacacgaag catgaaaatt ctcagcctgc gcctgaaaaa cctgaactca ttaaaaggcg
     5221 aatggaagat tgatttcacc cgcgagccgt tcgccagcaa cgggctgttt gccattaccg
     5281 gcccaaccgg tgcggggaaa accaccctgc tggacgccat ttgtctggcg ctgtatcacg
     5341 aaactccgcg cctttctaac gtttcacaat cgcaaaatga tctcatgacc cgcgataccg
     5401 ccgaatgtct ggcggaggtg gagtttgaag tgaaaggtga agcgtatcgc gcgttctgga
     5461 gccagaatcg ggcgcgtaac cagccggacg gtaatttgca ggtgccacgc gtagagctgg
     5521 cgcgctgcgc cgatggcaaa attctcgccg acaaagtgaa agataagctg gaactgacgg
     5581 cgacgttaac cggactggat tacgggcgct tcacccgttc gatgctgctt tcgcaggggc
     5641 aatttgctgc cttcctgaat gccaaaccca aagaacgcgc ggaattgctc gaggagttaa
     5701 ctggcactga aatctacggg caaatctcgg cgatggtttt tgagcagcac aaatcagccc
     5761 gcacagagct ggagaagctg caagcgcagg ccagcggcgt cgcgttgctc acgccagaac
     5821 aagtgcaatc gctgacagcg agtttgcagg tacttactga cgaagaaaaa cagttactta
     5881 ccgcgcagca gcaagaacaa caatcactaa actggttaac gcgtctggac gaattgcagc
     5941 aagaagccag ccgccgtcaa caggccttgc aacaggcgtt agccgtagaa gaaaaagcgc
     6001 aacctcaact ggcggcgctc agtctggcac aaccggcacg aaatcttcgt ccgcactggg
     6061 aacgcatcgc agaacacagc gcggcgctgg cgcatactcg ccagcagatt gaagaagtaa
     6121 atactcgctt acagagcaca atggcgcttc gcgcgagcat tcgccaccac gcggcgaagc
     6181 agtcagcaga attacagcag cagcaacaaa gtctgaatgc ctggttacag gaacacgacc
     6241 gcttccgtca gtggaacaac gaactggcgg gttggcgtgc gcagttctcc caacaaacca
     6301 gcgatcgcga gcatctgcgg caatggcagc aacagttaac ccatgcagag caaaaactta
     6361 atgcgcttgc ggcgatcacg ttgacgttaa ccgccgatga agttgctagc gccctggcgc
     6421 aacatgctga gcaacgccca ctgcgtcagc gcctggtcgc gctgcatgga cagattgttc
     6481 cccaacaaaa acgcctggcg caattacagg tcgctatcca gaatgtcacg caagaacaga
     6541 cgcaacgtaa cgccgcactt aacgaaatgc gccagcgtta taaagaaaag acgcagcaac
     6601 ttgccgatgt gaaaaccatt tgcgagcagg aagcgcgcat caaaacgctg gaagctcaac
     6661 gtgcacagtt acaggcgggt cagccttgtc cactttgtgg ttccaccagc cacccggcgg
     6721 tcgaggcgta tcaggcgctg gagcctggcg ttaatcagtc tcgattactg gcgctggaaa
     6781 acgaagttaa aaagctcggt gaagaaggtg cgacgctacg tgggcaactg gacgccttaa
     6841 caaagcaact tcagcgtgat gaaaacgaag cgcaaagcct ccgacaagat gagcaagcac
     6901 ttactcaaca atggcaagcc gtcacggcca gcctcaatat caccttgcag ccacaggacg
     6961 atattcaacc gtggctggat gcacaagatg agcacgaacg ccagctgcgg ttactcagcc
     7021 aacggcatga attacaaggg cagattgccg cgcataatca gcaaattatc cattatcaac
     7081 agcaaattga acaacgccag cagcagctct taacggcatt ggcgggttat gcactgacat
     7141 tgccacagga agatgaagaa gagagctggt tggcgacgcg tcagcaagaa gcgcagagct
     7201 ggcagcaacg ccagaacgaa ttaaccgcgc tgcaaaaccg tatgcagcaa ctgacgccga
     7261 ttctggaaac gttgccgcaa agtgatgaac tcccgcacag cgaagaaact gtagcgctgg
     7321 aaaactggcg gcaagttcac gaacaatgcc tcgcgttgca aagccagcag caaacgttgc
     7381 agcaacagga ggctctggca gcgcaaagtc tgcaaaaagc ccaggcgcag tttgacaccg
     7441 cgctacagga cagcattttt gacgatcagc aggcatttct tgcggcgtta atggatgagc
     7501 agacgctaac gcagctggaa cagctgaaac agaatctgga aaaccagcgc cgtcaggcgc
     7561 aaacgctggt cactcagaca gctgaaacgc tggcacagca tcagcaacac cgacctgacg
     7621 ggttggctct cactgtgacg gtggagcaga ttcagcaaga gttagcgcaa actcagcaaa
     7681 agttgcgtga aaacaccacc agtcagggcg agattcgcca gcagctgaag caggatgcag
     7741 acaaccgtca gcaacaacaa accttaatgc agcaaattgc tcaaatgacg cagcaggttg
     7801 aggactgggg atatctgaat tcgctaatag gttccaaaga gggcgataaa ttccgcaagt
     7861 ttgcccaggg gctgacgctg gataatttag tccatctcgc taatcagcaa cttacccggc
     7921 tgcacgggcg ctatctgtta cagcgcaaag ccagcgaggc gctggaagtc gaggttgttg
     7981 atacctggca ggcagatgcg gtacgcgaca cccgtaccct ttctggcggc gaaagtttcc
     8041 tcgtcagtct ggcgctggca ctggcgcttt cagatctggt cagccataaa acgcgtattg
     8101 actcgctgtt ccttgatgaa ggttttggca cgctggatag cgaaacgctg gataccgccc
     8161 ttgatgcgct ggatgccctg aacgccagcg gcaaaaccat cggtgtgatt agccacgtag
     8221 aagcgatgaa agagcgtatt ccggtgcaga tcaaagtgaa aaagatcaac ggcctgggct
     8281 acagcaaact ggaaagtatg tttgcagtga aataactatt cagcaggata atgaatacag
     8341 aggggcgaat tatctcttgg ccttgctggt cgttatcctg caagctatca ctttattggc
     8401 tacggtgatt ggtagccgtt ctggtggttg tgatggtggt atgaaaaaag tcattttatc
     8461 tttggctctg ggcacgtttg gtttggggat ggccgaattt ggcattatgg gcgtgctcac
     8521 ggagctggcg cataacgtag gaatttcgat tcctgctgcc gggcatatga tctcgtatta
     8581 tgcgctgggg gtggtggtcg gtgcgccaat catcgcactc ttttccagcc gctactcact
     8641 caaacatatc ttattgtttc tggtggcgtt gtgcgtcatt ggcaacgcca tgttcacgct
     8701 ctcttcgtct tacctgatgc tcgccattgg tcgactggta tccggctttc cgcatggcgc
     8761 attttttggc gtcggcgcga tcgtgttatc aaaaattatc aaacccggca aagtcaccgc
     8821 cgccgtggcg gggatggttt ccgggatgac agtcgccaat ttgctgggca ttccgctggg
     8881 aacgtattta agtcaggaat ttagctggcg ttacaccttt ttattgatcg ctgtttttaa
     8941 tattgcggtg atggcatcgg tctatttttg ggtgccggat attcgcgacg aggcgaaagg
     9001 gaagctacgc gaacaatttc actttttgcg cagcccggcc ccgtggttaa ttttcgccgc
     9061 caccatgttt ggcaacgcag gtgtatttgc ctggttcagc tacgtaaagc catacatgat
     9121 gtttatttcc ggtttttcgg aaacggcgat gacttttatt atgatgttag ttgggctagg
     9181 gatggtgctg gggaatgtgc taagtggccg gatttcagga cgttattcac cactgcgcat
     9241 tgcggcagta actgacttta ttattgtgct ggcactgctg atgctctttt tcttcggcgg
     9301 catgaaaaca acgtcgctta tttttgcatt tatttgttgc gcgggattat ttgccctttc
     9361 agcaccactg caaatattgt tactgcaaaa tgccaaaggc ggagagttat taggtgccgc
     9421 aggtgggcaa atagcgttta acctcggtag cgccgtcggc gcatattgcg gtggtatgat
     9481 gctgacgctg gggctggcat ataattacgt ggcgctacct gccgccctgc tttcgtttgc
     9541 tgcgatgtcg tcgttgctgc tgtatggtcg ctataagcgc cagcaagcgg cggatagtcc
     9601 ggtgctggcg aaaccagtgg ggtagattgc agtcacggag cacgttgctt attgccggat
     9661 gcggcgtgaa cgccttatcc gccctacgcg gttctggcac attttgcagg cctgataaga
     9721 cgcggcaagc gtcgcatcag gcatgatgag ccaattgcct ggttttttta ctcttgtggc
     9781 cataaccatg cagcgccgcg tacgccgctg gaatcaccgt gcttcgcctt acgcaccggc
     9841 gtttcacatt cgccgccgaa gacaaattgt ttaatcaact gcggaaccgt ttgatataaa
     9901 cggtctacat tgctcatccc gccccccagg acaatcacat ccggatcgag aatattcacg
     9961 acatgtgcca gcgattttgc cagccgcagc tcgtagcgac gcaatgccag ttccgctacc
    10021 ggatcgcttt cttcaaccag gcggataatt tcactgcctt tcagcgcatg tccgctcaaa
    10081 cgatgataat ccgtcgcgaa tcccgtgccc gaaataaagg tttcaataca accttgttta
    10141 ccgcaataac aagggacttc ctcgcgataa cgcagttcgt cttcgtccat ccacggtagc
    10201 ggattgtgtc cccactcacc tgccgtgcca ttgccgccga tatgcgcccg cccattgaat
    10261 gccacgcccg cgccgcatcc cgtgccgata atcacggcaa ataccgtctg cgctcccgct
    10321 gccgcgccat ctactgcttc tgaaaccgcc agacagttgg cgtcatttgc cagccgcact
    10381 tcccgctgca accgcgcgct taagtcttta tcgaatggct gaccgttgag ccaggttgaa
    10441 ttggcattct tcaccacgcc ggtgtaaggc gaaattgagc caggaatgcc catacctacc
    10501 gttccgcgca gccctgtcgc ctgctccgcc atatcaacca acgtggcgat cgtttcaata
    10561 gtctgccggt aatcatcacg cggcgtgggc agacgatggc ggtacaactg ctcccctgca
    10621 tcgcccagtg caatcacttc agttttggtg ccgcctaaat cgatacctat acgcacggta
    10681 ctctccttat ttttttcaat atcaatagcg tagagacgga caaccggatt ggcaatgcaa
    10741 gcccgccgac aattcgttat catgcccgct aaatttaacg acaaggccgt ggaaattatc
    10801 atgctgtggt tcaaaaattt aatggtttac cgtcttagcc gcgagatttc gctgcgtgca
    10861 gaagagatgg aaaaacagct agcctcgatg gcatttaccc catgcggcag ccaggacatg
    10921 gcaaagatgg gctgggttcc tccgatggga tcgcacagcg atgcgttaac gcacgttgcc
    10981 aatggtcaaa ttgttatctg cgcgcgcaaa gaagaaaaaa tcctcccgtc tccggtgatt
    11041 aaacaggcgc tggaagcgaa aatcgccaaa ctggaagcgg aacaggcgcg taagctgaag
    11101 aaaaccgaaa aagattcgct gaaagacgaa gtgctgcatt ctctgctgcc gcgtgctttc
    11161 agccgtttta gccagacaat gatgtggatc gacacggtta acggtttgat tatggtggac
    11221 tgcgccagtg ccaaaaaagc ggaagatacg ctggcattac tgcgtaaaag cctggggtcg
    11281 ttaccggttg taccattgag catggaaaat ccgattgaac tgacgctgac cgaatgggta
    11341 cgctccggca gcacagcaca gggcttccag ctgcttgatg aagccgagct gaaatcactg
    11401 ctggaagatg gcggcgtgat ccgcgcgaag aaacaagatc tgaccagcga agagatcacc
    11461 aaccacattg aagccggaaa agtggtgact aaactggcgc tcgactggca gcagcgcatt
    11521 cagtttgtga tgtgcgacga tggttcgctc aagcgtctga agttctgcga cgagctgcgc
    11581 gatcaaaacg aagatatcga ccgtgaagat ttcgcccagc gttttgatgc cgatttcatc
    11641 ctgatgactg gtgaactggc agcgttaatt caaaacctga ttgaaggatt aggtggcgaa
    11701 gcacaacgtt aattgctgat ttacctttaa tgccggatgc gacgcctgcc gcgtcttatc
    11761 cggcgtacga agctacacca ggcatataat tattcgctac ggcgggcaat aatttttagc
    11821 gcagcaatat tatgcgtttt acgctgtaac ttgctccagg ggcgttgtgt cattgttttt
    11881 cctcaagccg gtgacgcgct tcactacgaa ttttccgcgt tgccccggtt ttcactaaat
    11941 cccgtaactg cgaaagagcg ggttgatcga caaataaaag taatgatggc tcctctttta
    12001 gccacaccgt tttcacatcg gcagccagct gtggattatt aatagccagc gaccgatcct
    12061 gagactccgt caatgtcgtt aacaacatcg gggtatgtgc gttgcgcaat gccgtttggc
    12121 gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct gatttatctt
    12181 ttatcgtcgc cagttccgca acaatatctg gagatcttcg ctgatattta tatcaacctt
    12241 gatgaattat gaatatatcg gaaatcgaaa aaatcccagc aaaggaaata ttattatggg
    12301 ttaaagagag gacatttcag ataaatgccc tcccgttcaa ggggaaggcg aggaattaca
    12361 gatagcggca cagataagag gtgggttcgg caacttgcag atgaaactcg ctgtgaccgg
    12421 gaacattaaa caccgaaccg gcttcataca cttgccagtc ggtcgcgtca ggcagtaaca
    12481 cattcagcgc gccactgatt accgtcatct cttcaggctc agcggtgctg aaggtgtatt
    12541 caccttcaac cataacaccc acgctggcgc gaccagtgct gctgctggaa aagccgatcg
    12601 atttcacttt gccggaaaag tactcattac tttgaagcat aaactggccc tgtctggtag
    12661 ttgaacatct acttcactat aggggccaga ggcgctggct gtcacgcaaa attacatcag
    12721 taattccgca gccagccgtg caatcaatac gttagagagc aaaacaggaa catcgagctg
    12781 tttttgcagt aaatcgcgat gacgctggtt aaatcccaaa caatccagca tgatgacatc
    12841 tgcacctttt tccagtaatt cttttccggc atcaatgatt ttttgttccg aatcatgaat
    12901 ggggttaccc aatgaaaata ctggcggttt ctgcaaaatt tgccattttt gcgcctgaac
    12961 ggtcagcaac tcctcaaccg gaacgataac ccccacctga tgatcttcaa caatagaaga
    13021 aaccagcgga ggcaatattc gcgacggctc aagaaagatc gtattacgcg cagtcatact
    13081 actaatgttt gctgtactca ttaataaaat gacgtcataa ccctgattat cgagcacttc
    13141 aaccacacct tgcaggtcac gctccacttt gcgacgcgaa acatgggcca gctggttgtc
    13201 atttaataat gtgagaattg tgtcttcgcc tgcttctggc gcgtactccg ccatcacttc
    13261 ttcgcggctt aacttcccca gcaggctatg atgggaaata ttatcttcgt caatgtattc
    13321 tgtcaggagc ggcaaaactt cctgcatagg tacaatgccg atggtcagga tcgccaacga
    13381 cgcactcatt gttcttccac cttactttta tttcgtcgtt aattaccggg caagcgctaa
    13441 gcaccactgc tgacacttac ctgtgctaca aatgacaaaa agcgtagcag cagacctgca
    13501 cggcataatg taaagattcc aaatgattcc agtaatggat tgttattgtt taatattcta
    13561 attatttgaa aaacatgaat tgtgaaaaat gtgacgcaga tcccttgtgg cggcactcag
    13621 aatcccttcc gagcagtctg atgcggtcac tctttgtcag ggtcctcata gcgttttttc
    13681 gcatccatcg cttcctgagc ggtcggatgt tcactgatca acgagtctgg tttaggatga
    13741 tcggctctga gttggtacca ggttaccgtc tgtcctggtt ttcctttttc aatcgtcact
    13801 atatatgctt cacgaggata aggcggtttc gttggcatcg ttcttcctta tttcacggga
    13861 tgaacgttaa gtataggcgc tcgaaaatca acaattgatc gtctgtgcca gggcactgcg
    13921 aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg atgataatat gcgcaacttc
    13981 gcgataaagc gcatcgcgtt cttccagcac ttcctgaact tcttcgctca gcggtttccc
    14041 cgttaaggtt ggccgtaaat cttcttccgg tgcagcttgc agtcggttaa ccaggactga
    14101 tactggcgca cacaaataaa ccacgatccc gttattttgc ataaagtgac gattaaattc
    14161 cgtcaggata atgccgccgc ccgtggcgat aacggtggat ggcgcagtta ccgcttccag
    14221 cgccgccgtt tctctggcgc ggaatcccgc ccactcttcc ctttcgacga tctccgcgac
    14281 cgtcatattg agctgtgatt gcaaccactg gtcggtatcg acaaatcgac ggttaagcga
    14341 atcggcaagg gccattccga ccgttgtttt accacagccc cgaggcccga tcagaaaaag
    14401 aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg cggcgaaagc cggtgtcatg
    14461 agaatagcga tcataccatc aaactagtac aatttcgatt gtaaagaaaa aattccactt
    14521 aaagtgaaaa ttttaataca tcccttacta taccaataaa ttttcaagaa tgaagtgtaa
    14581 ataatatatt acattcagcc acgactacgt tgcacttcca gccaccactt ctccagctcc
    14641 gcggcaaagg cctggcggtc acgttgtgaa aggctatctg gtccgccggt ctggatccca
    14701 ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc gctcacgaat ggtcgctggc
    14761 gtgtaacgtt cgccgcgcgg attaagcgcc gcagcgcctt tctcgatggc ttcagcagcc
    14821 aaaggtatat ctgcggtgat caccaaatcg cccgcttcac actgccggac aatttcgtta
    14881 tcggcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa taaatcgcga tggcggcacg
    14941 cgtaaactct gatttgctac cagtaccagc ggcatctgca tacgttccgc cgcgcgatac
    15001 aaaatctctt taattacatt gggacacgcg tcggcatcca cccaaattgt cataaagtca
    15061 tcctttgttg ggtaatcctc tattgtgtcg cgcttttgcc ttccggcata gttctgttta
    15121 tgcttctgcc agcgattatc aaaacaatga atttcacggc aggagtgagg caatggaaaa
    15181 gaaaatcggt tttattggct gcggcaatat gggaaaagcc attctcggcg gtctgattgc
    15241 cagcggtcag gtgcttccag ggcaaatctg ggtatatacc ccctccccgg ataaagtcgc
    15301 cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg gcgcaagaag tggcgcaaat
    15361 cgccgacatc atttttgccg ccgttaaacc aggcgtcatg attaaagtgc ttagcgaaat
    15421 cacctccagc ctgaataaag actctctggt cgtttctatt gcagcaggtg tcacgctcga
    15481 ccagcttgcc cgtgcgctgg gccatgaccg gaaaattatc cgcgccatgc cgaacactcc
    15541 cgcgctggtt aatgccggga tgacctccgt aacgccaaac gcgctggtaa ccccagaaga
    15601 taccgctgat gtgctgaata ttttccgctg ctttggcgaa gcggaagtaa ttgctgagcc
    15661 gatgatccac ccggtggtcg gtgtgagcgg ttcttcgcca gcctacgtct ttatgtttat
    15721 cgaagcgatg gccgacgccg ccgtgctggg cgggatgcct cgcgcccagg cgtataaatt
    15781 tgccgctcag gcggtaatgg gttccgcaaa aatggtgctg gaaacgggag aacatccggg
    15841 ggcactgaaa gatatggtct gctcaccggg aggcaccacc attgaggcgg tacgcgtact
    15901 ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg acgaagtgta tggaaaaatc
    15961 agaaaaactc agtaaatcct gatgactttt gccggacgtc aggccgccac ttcggtgcgg
    16021 ttacgtccgg ctttctttgc tttgtagagc gccaaatctg ccgatttcaa ccactcgcga
    16081 tagtgactca tttgtgggtt cagcggcgca acccccacac taatccgtaa catcacctgc
    16141 ggcgtattcg gcaggcgtaa cgtatttaat ccttcatgca ctcgtaacat ggcggtaatg
    16201 gcgctctcag ctggcgtacc ggacatgatt accgcaaact catcgccgcc aaaccgacca
    16261 atcacatcgc taccgcgcag ggttatttgt aattgtcggg taagcgccac aatcgcttca
    16321 tcgcccacat catggcccca ggtatcgttg atgctcttga aatggtcgat atcgataatc
    16381 agtaatgtcg catcgcggtt atgccgccga cagttatcaa attcattgcg taacatagtt
    16441 tcccaatgac gtcggttata cacgcccgtc ataccgtcgc gggtactcat gacctgcaac
    16501 ctgcgtttat gttccgccag tttagtcgcc gtctggtagc tgacccagcc aaacagcaga
    16561 ggataaatga cgataatggg aagggagagc caccattcca gcggcgcact attgaacgac
    16621 acggtgatgc ccgtcagttc aagggtgaca agacaggaaa ccaccatcaa taccagaccc
    16681 gcgacaaaca gacgggggcc gcctgccccc atcaaattca gacacataat catcaacatc
    16741 gcggtggaag gcagcacgtt tacgcccatt acgcctaccc acattcccgc taatactgca
    16801 tcggttttta agttgtaaat ttcccggcta agcggatcga cggccctgct cgctatctgc
    16861 caggctaaat gcggccagac gaacgcccag ccgaccaaca ccagccacca ccagcccggc
    16921 ggcgggtgcg aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag gccaaccgcg
    16981 cggggtagtc tgacgcgacg ggcgaagcgc agcccggatc gctgatgttc gttttgagga
    17041 gttaaaggag gttcctcccc atgcgccgcc gcttttttga aaaagttttc atcattcatt
    17101 atttttggga acattcagaa acaattttcc caaattatag agacggggct aaaggcagaa
    17161 gtatgaaatt tcgggtgagc cattttcgta gctcacccgt tagcactatc aggtatcagg
    17221 cggctttctt gaggcaggcg ctcataaact tattacgatc atcacctttc agagattgtt
    17281 gcgttgcttg attattacat tcgcgcatct tttgctgctg tggcgtcaaa cttttttcac
    17341 caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga gcatcccctt
    17401 tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt tgcggggtta
    17461 aggttctctc ggcagcgccg acggtggtta aaaaaaccag accgaaaagc aaggtaacca
    17521 gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg tatgcgcccg
    17581 tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc gaaaattcac
    17641 tgccgggcgc ggttttattt cagccccagg gcggctttca tggtgtagaa gagatcggtc
    17701 tggtcggtta gtccaacaac attggcggca tgcgggccat acgccgcaat acgcaactga
    17761 ctgccggtat gttcttgtga atcctcttcg gagttcccgt aactcatcac catcactgcg
    17821 ccatctttgg tatttagtgc ctgggtgagg cccggcgctt tggtgtccgg cgcgacaatc
    17881 tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttgccatc ctttttagcg
    17941 aattccagcg cacgttgtac ggcttcatcg agatcgaccg tctcgccaat ttgcccacaa
    18001 ggattcgcag cgtgatcctg tttatcgatg gatgcacctt caacttgcag gaaaaagcct
    18061 ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgcgccag ggtcggtacg
    18121 ctgtcattac gttgcggatt aggcgtacag gtaactgcgg gcttgtcgat attgccgtgg
    18181 tacgttgctt tcggtcctag ccagcgcact ggcatattgc cgtcagcaaa caggccaagc
    18241 aggggttttt gctgattcgc ttccgtcacc gcatttagcg atgcagcatc actcaccagc
    18301 tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc gccagcggtt
    18361 gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa catcggcacg ggcgttaagc
    18421 agctgttcgg taatcgatcc tctcccgcct ttttccagag cgttatccgg acatttttca
    18481 ctggtcgcgc tcggaccgta gcatttgcgc gaggtcacat gcgccaccag cgcggcgggc
    18541 gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc ggcttttgcc
    18601 atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc accgttatag
    18661 gttttgacac cagttgacca ggcggttgct gatgcagccg agtcggtaac gtagtccggt
    18721 ttgccggttt ttttattcag cgcatagtgt gtgtattgtc cggtaagcgg taaggcatcg
    18781 atacctttaa aaaagccgcc cgcaccttcg gcataattgc gtgcagcagt aatttccgag
    18841 tcccccatcc catcgccaat cagcaaaata atatttttgg caggtttatc gctaagagaa
    18901 tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg tgcagtaata
    18961 tctccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc ttttgtcaca
    19021 ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac tttattttct
    19081 ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg ccgtgacagc
    19141 tttatgacag ctgttgaaaa gattaacttt ttactgacga ggatgcttca ataacttctt
    19201 tacataattg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg gctttacctc
    19261 gtacagcgcc ccctctacct gatcaatcaa cctttgttgg tcattttgcg ccatattgcg
    19321 aagcattgca gtgacgataa tctccaaagc ttctacctgc gcgcacagtt ctttcgactc
    19381 ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca tgtctgtatt
    19441 tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag tgtctggaag
    19501 tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgttt
//
LOCUS       DAOTRK010000087        19532 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.087, whole genome shotgun sequence.
ACCESSION   DAOTRK010000087 DAOTRK010000000
VERSION     DAOTRK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.087"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            43..147
                     /locus_tag="QIE41_002959"
     CDS             43..147
                     /locus_tag="QIE41_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDS3531827.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            458..2656
                     /gene="speF"
                     /locus_tag="QIE41_002960"
     CDS             458..2656
                     /gene="speF"
                     /locus_tag="QIE41_002960"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDS3531828.1"
                     /translation="MSELKIAVSRSCPDCFSTHRTCVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIIEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENGPGILVTQSVHKQQAGFSQTSQIHKKDNHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2653..3972
                     /gene="potE"
                     /locus_tag="QIE41_002961"
     CDS             2653..3972
                     /gene="potE"
                     /locus_tag="QIE41_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDS3531829.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCVIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4029..5669)
                     /gene="pgm"
                     /locus_tag="QIE41_002962"
     CDS             complement(4029..5669)
                     /gene="pgm"
                     /locus_tag="QIE41_002962"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDS3531830.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASISGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5695..6240)
                     /gene="seqA"
                     /locus_tag="QIE41_002963"
     CDS             complement(5695..6240)
                     /gene="seqA"
                     /locus_tag="QIE41_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDS3531831.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6425..7189
                     /gene="ybfF"
                     /locus_tag="QIE41_002964"
     CDS             6425..7189
                     /gene="ybfF"
                     /locus_tag="QIE41_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDS3531832.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWEQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7329..7622
                     /gene="ybfE"
                     /locus_tag="QIE41_002965"
     CDS             7329..7622
                     /gene="ybfE"
                     /locus_tag="QIE41_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDS3531833.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7762..8292
                     /gene="fldA"
                     /locus_tag="QIE41_002966"
     CDS             7762..8292
                     /gene="fldA"
                     /locus_tag="QIE41_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDS3531834.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8502..8588
                     /gene="uof"
                     /locus_tag="QIE41_002967"
     CDS             8502..8588
                     /gene="uof"
                     /locus_tag="QIE41_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDS3531835.1"
                     /translation="MIRIISRANSVTSRNEVNRLVTGQIPHD"
     gene            8581..9027
                     /gene="fur"
                     /locus_tag="QIE41_002968"
     CDS             8581..9027
                     /gene="fur"
                     /locus_tag="QIE41_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDS3531836.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9112..9438)
                     /locus_tag="QIE41_002969"
     CDS             complement(9112..9438)
                     /locus_tag="QIE41_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531837.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9488..10894)
                     /gene="chiP"
                     /locus_tag="QIE41_002970"
     CDS             complement(9488..10894)
                     /gene="chiP"
                     /locus_tag="QIE41_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDS3531838.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGEAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11343..13007)
                     /gene="glnS"
                     /locus_tag="QIE41_002971"
     CDS             complement(11343..13007)
                     /gene="glnS"
                     /locus_tag="QIE41_002971"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDS3531839.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13210..15156)
                     /gene="nagE"
                     /locus_tag="QIE41_002972"
     CDS             complement(13210..15156)
                     /gene="nagE"
                     /locus_tag="QIE41_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDS3531840.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15489..16289
                     /gene="nagB"
                     /locus_tag="QIE41_002973"
     CDS             15489..16289
                     /gene="nagB"
                     /locus_tag="QIE41_002973"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDS3531841.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16349..17497
                     /gene="nagA"
                     /locus_tag="QIE41_002974"
     CDS             16349..17497
                     /gene="nagA"
                     /locus_tag="QIE41_002974"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDS3531842.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            17506..18726
                     /gene="nagC"
                     /locus_tag="QIE41_002975"
                     /gene_synonym="nagR"
     CDS             17506..18726
                     /gene="nagC"
                     /locus_tag="QIE41_002975"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDS3531843.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18774..19526
                     /gene="nagD"
                     /locus_tag="QIE41_002976"
     CDS             18774..19526
                     /gene="nagD"
                     /locus_tag="QIE41_002976"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDS3531844.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         4765 a         4962 c         4924 g         4881 t
ORIGIN      
        1 aataactcta ccttcaactg acacagcaag aggtaaaggt aaatggaaaa taataaccgc
       61 ttaatgcctc atataaggcg gacaacccat atcatgaagt ttgcccatcg taatagtttt
      121 gactttcatt tctttaatgc ccgctagtct tctgactaaa gggcacccca acgtacaggt
      181 ctccctgact ttaagcatta caggttaata cctgtatccc tcggtgctca tatactgcta
      241 acccttttaa actctaaata attcgagtcg cagcacttgc aacttgaggt atgacgagta
      301 tagccagtta ccgggctggt ctgggttatt gcatctacaa aaagcaaact actgatttat
      361 ttatcagcgg tggggttttg ctttttttcc ggcgtgatcg atttctcctt tgagaaattg
      421 aggacctgct attacctgaa ataaagagat gaacaaaatg tcagaattaa aaattgccgt
      481 tagtcgttct tgcccggatt gtttttcaac tcatcgcaca tgcgtgaata ttgacgaaag
      541 taattatatt gacgttgccg ccattatttt atcggtcaat gatgtggaac gtggaaaact
      601 cgatgaaata gacgctactg gctatggcat tcctgttttt attgcaacgg aaaatgaaga
      661 acgtgtaccc gcagaatatc tgccacgtat ttctggtgtc ttcgaacatt gcgaatcacg
      721 taaagagttt tatggacgtc agttagaaac cgctgcaagc cattatgaaa cgcaactgcg
      781 cccgcccttc ttccgcgcac tggtcgatta tgttaatcag ggcaatagtg cttttgactg
      841 ccctggtcat caggggggtg agtttttccg tcgccatccg gcaggcaatc agttcgtgga
      901 atactttggt gaggcgctgt tccgtgctga cttgtgcaac gccgacgtag cgatgggcga
      961 tctgctgatt cacgaaggcg cgccatgcat tgcacagcaa catgcggcta aagtctttaa
     1021 tgccgataaa acctatttcg ttttaaacgg tacttcatct tctaataaag tggtcttaaa
     1081 cgcattgctg acgccgggcg atctggtgtt gtttgaccgc aataaccata aatctaacca
     1141 ccacggtgcg ttgctacagg ctggcgcgac accggtttat ctggaaaccg cacgtaaccc
     1201 gtatggtttt atcggtggta ttgacgctca ctgttttgaa gaaagttacc tgcgtgagct
     1261 gattatagaa gttgcaccgc agcgggcaaa agaggctcgt cctttccgcc tcgccgttat
     1321 tcagctaggc acttacgacg gtacgattta taacgcccgt caggtggtgg ataaaattgg
     1381 tcatctgtgt gactacatcc tctttgactc cgcatgggtc ggctatgagc agtttatccc
     1441 gatgatggcg gactgttcac cactgttgct ggatcttaat gagaacggtc cgggtattct
     1501 ggttacgcaa tccgtacata aacagcaggc tggtttttct caaacttcac aaatccataa
     1561 aaaagacaac cacatcaaag ggcaacagcg ttatgtaccg cacaaacgca tgaacaacgc
     1621 ctttatgatg cacgcctcca ccagcccgtt ctatccgctg tttgccgcac tggatatcaa
     1681 cgccaaaatg catgaaggtg tcagcggtcg taatatgtgg atggattgtg tggtaaatgg
     1741 cattaatgcc cgcaaactga tcctcgataa ctgtcagcat attcgtccgt tcgtacctga
     1801 actggtggat ggtaaaccct ggcagtcgta tgaaacagcg caaattgcgg ttgatctgcg
     1861 cttcttccag tttgtaccag gggaacactg gcattctttt gaaggctatg cagagaatca
     1921 atactttgtt gatccgtgca aactgttgct gacaacccca ggtattgatg cacgtaacgg
     1981 cgaatatgaa gcgttcggtg tacccgcgac gattcttgct aatttcctgc gcgaaaatgg
     2041 cgtagtgccg gaaaaatgcg atcttaactc catcctcttc ctgctgactc cagcagaaga
     2101 tatggccaaa cttcagcaac ttgttgccct gctggtacgc ttcgaaaaac tgctggaatc
     2161 tgatgcgccg ctggcggaag tgctaccttc catctacaaa cagcatgaag agcgctacgc
     2221 cggttatacg cttcgtcagt tgtgccagga aatgcatgat ttgtatgccc gccacaacgt
     2281 gaaacaactg caaaaagaga tgttccgtaa ggaacacttc ccacgcgtca gcatgaatcc
     2341 gcaagaagct aactacgcct atttacgcgg tgaagtggag ctggttcgtc tgccggatgc
     2401 agaaggccgt atcgctgcag aaggtgcgct tccttatcct ccgggtgtgc tgtgtgttgt
     2461 tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg aagaagggat
     2521 caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag aacatgatgg
     2581 tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca ccctgttgaa
     2641 aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca gttaaccata
     2701 ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa gcttgccgaa
     2761 gttgggacaa tttcaattat ctcctggctg gtgacagccg taggctcaat ggcactggca
     2821 tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gtggtatggg cggttatgcc
     2881 gagtatgctt tcggtaaatc cggtaacttt atggcgaact atacctacgg cgtctcactg
     2941 ctgattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga actgctcggc
     3001 gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg gatttgtacc
     3061 gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac cgtgtggggg
     3121 gtcattattc cggtggtagg cctgtgcgtt atcggctggt tctggtttag cccgacgctt
     3181 tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcggtagg ttcttccatc
     3241 gctatgacgc tgtgggcttt tcttggtctg gagtctgcgt gtgcgaatac tgatgtagtg
     3301 gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg tgcggcggtg
     3361 atttatatcg tctccaccaa cgtgattgcc gggattgtgc ctaatatgga gctggcaaat
     3421 tcaacggcac catttggcct ggccttcgca cagatgttca cgccagaagt gggtaaagtc
     3481 attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca gttcaccatt
     3541 gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt ctcccgtgta
     3601 accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca gagcgggttg
     3661 gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt taacctggcc
     3721 gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat tattcagaag
     3781 gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt tgttggcgcg
     3841 atgtacagct tttatgcgct gtactcatcc ggggaagaag ccatgctata cggttccatc
     3901 gtgaccttcc tcggttggac actgtatggt ctggtctcac cacgctttga actgaaaaat
     3961 aaacacggtt aattaagcaa agcaaaaaag ggcgatcttc cgaccgccct ttttcttatt
     4021 taatgtgttt acgcgttttt cagaacttcg ctaacaatct caaccgcttc tttctcgatc
     4081 tgcttgcgat gctcttcacc gaggaagctt tcgcaataaa tcttgtacgc gtcttccgtg
     4141 cctgacggac gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc gctgatagaa
     4201 gcaccgttgc ccggtgccgc cgtcaggcgc gcggtgatcg ggtcgcctgc cagggtgctg
     4261 gcgctcacca tttccggaga cagcttagac agcgccgctt tttgtgcgga agtcgcggct
     4321 gcctgcaaac ggttgtagct cggcgcacca aagcgttttg ccagttcgtt gtagtgttcc
     4381 tgcggattct taccggtgac agcggtgatt tccgccgcca gcagacacat aatgatgccg
     4441 tctttatcgg tggaccacgg cgtgccgtcg aaacgcagga aggaagcgcc tgcactctct
     4501 tcgccgccaa agccgaagct gccatcaaac aaaccatcaa caaaccattt gaaacctacc
     4561 gggacttcta ccagcttacg gcccaaatca ttgaccacac ggtcgatcat cgcagatgaa
     4621 accagcgttt taccgacagc aacatctttg ccccactgcg gacggtgctg gaacaggtag
     4681 ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac ctgccggagt gacgataccg
     4741 tgacggtcat aatccgggtc gttagcaaac gccagatcga acttatcacg cagtgccagc
     4801 agacccgcca tcgcacactc ggaggagcag tccatacgga tcgcgccgtc tttatcgagg
     4861 tgcataaagc ggaaggtttg atcgacctga tcgttaacga tggtcaggtt gagattgtaa
     4921 tactcgccaa tgcgcttcca gtattcgata ccggaaccgc ccagcggatc aacgccaagc
     4981 gtcaggcccg ctttctgaat cgccgccata tcaacgatat cggccagccc ttccacgaac
     5041 ggctgcacca gatcctgctc tttcacatga ccggatgcca tcgcttcgtc gagggagata
     5101 cgcttcacac ctttcaggcc atcggccatc agtgcgttgg ccctgtcttc caccactttg
     5161 gtgacgttgg tatcagccgg gccaccattt ggcgggttgt acttgatacc accatcttcc
     5221 ggcgggttat gggacggtgt aatcacgata ccgtctgcca gcgggccacc ttttttattg
     5281 tgaaccagga tggcattgga aacggcaggc gttggagtga agccattgtt ttcctgcaca
     5341 ataacatcaa cgccgttcgc tgccagcact tccagaacgg aaatgaacgc aggctcggac
     5401 agggcgtgag tatctttacc cacatagcaa gggccagtga tgccgttttt cgcacgttct
     5461 tcagcaattg cctgagcgat cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca
     5521 ctgccacggt gaccggaagt accgaatttc accgcgtgct ccgcattccc tgcttctggt
     5581 ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca
     5641 ggttggcctg cacgattgtg gattgccatt gctttgtcct ttgtctgcaa cgttttagat
     5701 agttccgcaa accttctcaa tcaattccgc cgggaattgc atcgactgca tgatgtgttc
     5761 gatcatgctg catttacggc cggtgttggt gttggtgatc acccaatacg gcgtgccagg
     5821 cacatgtttc ggcttggtct gattaccatt tttcagcagc gtttgttcat ctgccgcaaa
     5881 gtaaacgcgt gtacgaccgt gcaacgattc cgttgcctcg gcaaacgcct gggcgtcaag
     5941 agaatatagt gtagacaaca gcagcataaa gcgattgacc gctcgctttt gctctgcgta
     6001 ttcatccgaa agcagaagtt cacgcattgc gcgaaccttg tctttaatcg ttttgaccgg
     6061 cttcgcttcg acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg
     6121 ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat
     6181 atgcttagtg tggctggcaa tatagctgta gagttcatca tcaacttcaa tcgttttcat
     6241 cttaatccag tgcagtgtct tagctgaata caagtcgcca ggagtatatg aataaatccc
     6301 ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg gaataaacct tatttgttgc
     6361 agccctgtgg cacaatggtc aacataatac cctaacccga caacgcgaaa aaaagaactt
     6421 tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata attctcccat
     6481 cgttcttgtc catggtctgt ttggcagcct cgacaacctt ggcgtactag ctcgcgatct
     6541 ggtaaacgat cacaatatca tccaggttga tatgcgtaac cacggtcttt caccgagaga
     6601 tccggtaatg aattacccgg cgatggcgca ggatcttgtt gataccctgg atgcactgca
     6661 gatcgataaa gcaacattta tcggtcactc catgggcggt aaagcggtaa tggcacttac
     6721 tgcactggcc cccgatcgca tcgataaact ggtggcgatt gacatcgcgc cggtcgacta
     6781 tcacgtacgc cgtcatgatg agattttcgc ggctatcaac gcagtcagtg aatcggacgc
     6841 acaaactcgc cagcaagcag cagcaataat gcgccagcat cttaatgaag aaggggtgat
     6901 tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc cggtattgtg
     6961 ggaacagtat ccgcatattg tgggttggga gaaaatcccg gcatgggatc accctgccct
     7021 gtttatccca ggcggcaatt ctccgtatgt tagcgagcag tatcgtgatg atttactggc
     7081 gcaatttcca caggcgcggg cgcatgtgat tgctggcgcg ggtcactggg tccatgctga
     7141 aaaaccggat gcggtattac gcgctatccg tcgctatctc aatgattaac gcaactgatt
     7201 aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc atcgcgctga
     7261 tgtattatgg cgcgctatcc atccgggcag aggcctggct gattgtttcc cccgaagtca
     7321 ccaagatcat ggccaaagaa caaacggacc gtacgacatt agatctgttc gcgcacgagc
     7381 gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga tgaacagctg cgtattaata
     7441 aacgcaacca gctaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa ctgaagctga
     7501 acgcggaagc tgtcgaggcg ctgaacgagc tggcagaatc gcgcaatatg agccgtagcg
     7561 aactgatcga agagatgtta atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt
     7621 aaatttccac tttcatgtag cacagtgtgc agtcctgctc gtttgctgat tccgcaagac
     7681 tgcctgttct gctatgattg cctttatccg tgggcaattt tccaccccca tttcaataag
     7741 tttcaagagg ttatttcact catggctatc actggcatct ttttcggcag cgacaccggt
     7801 aataccgaaa atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc
     7861 catgacattg caaaaagcag caaagaagat ctggaagcat atgacattct gctgctgggc
     7921 atcccaacat ggtattacgg cgaagcgcag tgtgactggg atgacttctt cccgactctc
     7981 gaagagattg atttcaacgg caaactggtt gcgctgtttg gttgtggtga ccaggaagat
     8041 tacgccgaat atttctgcga cgcattgggc accatccgcg acatcattga accgcgtggc
     8101 gcaaccatcg ttggtcactg gccaactgcg ggctaccatt tcgaagcgtc aaaaggtctg
     8161 gcagatgacg accactttgt cggtctggct atcgacgaag accgtcagcc ggaactgacc
     8221 gctgaacgtg tagaaaaatg ggttaaacag atttctgaag agttgcatct cgacgaaatt
     8281 ctcaatgcct gatgtgatgc ggcgtagact catgtctacg ccgtattaat agataatgcc
     8341 aatcaaaata attgctacaa atttgtaact tttgctgttg tacctgtaca atgtcccggt
     8401 gttcaagtgg ccttgccgtt gtaaatgtaa gcagtgccac gtttttatta acaatatttg
     8461 ccagggactt gtggttttca tttaggcgtg gcaattctat aatgatacgc attatctcaa
     8521 gagcaaattc tgtcacttct cgtaatgaag tgaaccgctt agtaacagga cagattccgc
     8581 atgactgata acaataccgc cctaaagaaa gctggcctga aagtaacgct tcctcgttta
     8641 aaaatcctgg aagttcttca ggagccggac aaccatcacg tcagtgcgga agatttatac
     8701 aaacgtctga tcgatatggg tgaagaaatt ggtctggcta cggtatatcg cgtactgaac
     8761 cagtttgacg acgctggtat cgtcacccgc cacaattttg aaggcggtaa atccgtattt
     8821 gaactgacac agcaacatca ccacgatcac ctgatctgcc tcgactgcgg caaggtaatc
     8881 gaatttagtg atgactccat cgaagcgcgt cagcgtgaaa ttgccgcaaa acatggcatt
     8941 cgcctgacta accacagtct ctatctttac ggtcactgtg ccgaaggcga ttgccgcgaa
     9001 gatgagcacg cgcacgaagg caaataagcc agcctgaatg agaaaaagcc aacctgcggg
     9061 ttggcttttt tatacacggg aaataagaaa agcgagagtt acagctctca cttatttgtt
     9121 attactgcga atttcctgcc aaactttatc acaatcggct ttcaccgcct gatcatttcc
     9181 ggtttgcgtt gcacgcagac actgctgata atccagtacg cgtacacttt cctggtcggc
     9241 aaactgctga tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat
     9301 tttttctggc gaaccttgtg cggtgttgat acaggcgctg tatgcctctt tcagacggct
     9361 gtcttcctgt ggcgcggttg attgcgcaca agccaccagc cccgatgcca tcatggcgat
     9421 gagaatcagt tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg
     9481 tcgggcatca gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct
     9541 ggaaaatgtt gccgtaacca ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg
     9601 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat
     9661 ccaggctgta ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc
     9721 tctgccaggt agcgggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
     9781 ctggaagatt ccagtttttc aagtcataca tcgcaccgaa gaagaccgct ttttcgccgt
     9841 tggcgttgaa gtcagaacgg ttatcccacc agatatccag gcgaccgttc gaggaagcgt
     9901 aggttggagt catacgttgc agaaagtatc cctgctgacc gtcggcttta acccaggtgc
     9961 cttccagacg caaatccact acatcagccg cccggtaacc aaaggtcaac gcttgcagcc
    10021 aggcagtgcc gtcgtaaagg tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccgt
    10081 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    10141 caaagtattg atcgatatac ccttccgcct gaccaaatgc tgcttccagt acgaagttat
    10201 ttttgaagtc gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt
    10261 tctgataaaa ctcatccatt tccagatgcc acggtgcttt gtattcgttg gtccacatgt
    10321 aggagaaact caacgcacca gcttcgccgt aatcaaaatt cgccccggct tccgcaccct
    10381 gataagtacc cggcataaag ctccagtgcg gcgctaacag cgtctgacca gttggctgaa
    10441 tgtaacctgc cctcgcccac accggaccgt atttaaattt ggccgctgct ttatacaggc
    10501 ttataccgct tttgtcgccg gaccagtctt cgtcataggc tttattgctt tttgaaaacg
    10561 cgatttcgtt cgggtgagag ctgtcaccgt tttccgccat ttcaatcgcc gtaaacgcag
    10621 caatatcaag gccgaacata tcagcagcat aaccagactg aaaatcgagg ttggcgttcc
    10681 aggtggagtg agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc
    10741 gttcacgctg ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg
    10801 cgcgagcctc cggcattgcc ataaagcccg acattgctgt aacaccggcg atagccagcg
    10861 ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat
    10921 tgctgcacca aaggtgaaca gcgaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag
    10981 tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta
    11041 accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta
    11101 agtttgcagt tgaaaggctt gcagaagaat aaaaacgtga aattccagca agatgaaagc
    11161 ggttacattt tatttaatta tgttttgtga ttttaaattt gttttatgga taaattgcga
    11221 agcggaatta attcgcgaag aaaagcagat aaaaacgccg cagagcgcgg cgttttggat
    11281 tgtttgcatg tttcatgccg gatgcggcgt aaaagcctca cccggcctgg tagaacttaa
    11341 aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt
    11401 ttttccgccg tagaatggcg gctgtcgagg cagaaataac cttcacgctc aaactggaat
    11461 gctttacccg ctaccgcgtc tttcagagac ggttcagcaa agccctgttt gatcaccagc
    11521 gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttcgg cacgctgaac
    11581 agacgatcat acaggcggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata
    11641 acgcctttca ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta
    11701 cagaaaatgg tggtgatatt gccttcggca tctttctcca cgcgttcagc cttaatgacg
    11761 taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct
    11821 tcttcgcgga aatcggcgcg gtcgatccaa atctcaccgc taaacggcac ctgacggcta
    11881 cccatttccg gtttgttcgg atggttcggc atggtaacca tttcgccttc gccctggtag
    11941 ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggggc gttttcgttg
    12001 agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc
    12061 acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga
    12121 ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc
    12181 aggttcagct tacgcttgga catcacggtg tattccagat tcaggcgcga gaattcgtac
    12241 tggcgcgggt gaacaggaat ggtgatgttg tccagtaccc agtcgtacag acgacggttg
    12301 tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc gctgatgcaa
    12361 tgtgtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca
    12421 aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc catgtcgatt
    12481 ttcgcacgca ggcaggcttt accttcttca aaaccaccgg tacgcatttt ttcgaacagc
    12541 gccaggttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ctgcgtcagg
    12601 gtgccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc caggccttta
    12661 ttgatcagtt cgatcgcata ggcgtggagc tgatcaaagt aatcggagga gtaacggacg
    12721 ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc gacatactcg
    12781 atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc
    12841 tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc
    12901 ggctccggcg ggaaacgggt gtgtactgtg gtgtgcttac cactggccag atcttcatcg
    12961 atgatctgac gaataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca
    13021 aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta
    13081 gttagtgaaa atcgaaaatc tcactgagat tatggggatg actgatgaaa aaaaaagcgg
    13141 cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc gcatcaggta
    13201 taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg
    13261 agctttaatg ataaggccgc tgaaatcgtc gatattgctg caaaccaccg ggctaatcat
    13321 cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt gtcctgcgct
    13381 tacctgcgcg ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc
    13441 gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt
    13501 ggtattgaag attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg
    13561 tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc
    13621 cagtgccaca acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg
    13681 tacagcctgc ggttttgcta caggagcggc agttgccgga gttgcttcag ctgacgcagc
    13741 ggctaccgga ccacgggcaa caactttctt cattgcatcg ccaatggatt ccgctttcgc
    13801 gccaacaatc acctgaatag tctgtttgtt cagtttcact accccagaag cacccagacg
    13861 tttacacatc gcatcgttga cgcgagctga gtcaaccacg gtcaggcgca gacgagtaat
    13921 acaggcgtca attgctttca ggttgtcagt accgcccacc gcagcaatat agttggtcgc
    13981 cagttgagtc agaccttctt ctgtgttgct gttggcttct tcagtaacga tctcgtcttc
    14041 tttatcttca cggcccggcg ttttcaggtt gaacatacgg ataaccaaac tgaacaccac
    14101 gaagtagata gcgaagaaga caacacccat aaccagcagc atccagacgt tctggctggc
    14161 ggccggcagg ttatacatca acgcgtagtc gatagccccc gcagagaagg agaagcccgc
    14221 gtggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag
    14281 gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca caccggtcag
    14341 gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg
    14401 tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa
    14461 gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg
    14521 gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag
    14581 cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc
    14641 cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg
    14701 ccagacgtaa ccaaaaatgg ccgccagcac caggcagaag aagccggtgg caatcggcac
    14761 aaagcgtttg ccgccgaaga aactcaggaa gtccggcagt ttaatatcgg accaacggtt
    14821 ataagctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc
    14881 tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc
    14941 cgccgcacct gcgctgtctt tcgaccagct ggatgccacg ccgatggcga agattaatgc
    15001 gaggttatca aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg
    15061 ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga gctgtaacgc
    15121 cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta
    15181 tttggctcgt ttaaagccgt atttttattt tgctgcaaat tgtactgccg atgttctgta
    15241 atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgta tcgcaaatta
    15301 aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg
    15361 ctaaaccaga aaacttattt tatcattcaa aaaatcaggt cggattgacg cctgtctgcg
    15421 caaatccagg ttacgcttaa agatgcctaa tccgccaacg gcttacattt tacttattga
    15481 ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc
    15541 gccatatcgt caatcgtatc aatgcgttca aaccgactgc tgatcgtccg tttgttctgg
    15601 gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag
    15661 caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc
    15721 cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg
    15781 atattccagc agaaaacatc aaccttctca acggcaacgc cccggatatc gacgccgagt
    15841 gccgccagta tgaagaaaaa atccgttctt acggaaaaat ccatctgttt atgggcggtg
    15901 taggtaacga cggccatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc
    15961 gtatcaaaac actgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg
    16021 tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacattgctg gatgccgaag
    16081 aagtgatgat tctggtgctg ggtagccaga aagcactggc actgcaggcc gccgttgaag
    16141 gttgcgtaaa ccacatgtgg accatcagct gtctgcaact gcatccaaaa gcgatcatgg
    16201 tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat
    16261 tagaagcaga aaatatcaaa ggtctgtaat agttatccct gccctgacac cttgctcagg
    16321 gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac
    16381 cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt
    16441 ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct
    16501 ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtgcagt ttaacgatac
    16561 tgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg
    16621 ctgtactaac tatttgccga cgcttatcac taccagcgac gagctgatga aacagggcgt
    16681 gcgcgttatg cgcgagtacc tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga
    16741 aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc
    16801 tgatgccgcg ctggtcgatt tcctgtgcga aaatgccgat gtcattacca aagtgaccct
    16861 ggcaccggaa atggttcctg cagaagtcat cagcaaactg gcaaatgccg ggattgtggt
    16921 ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc gcgcggggat
    16981 tacctttgcc actcatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct
    17041 ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attattgctg acggcctgca
    17101 tgttgattac gccaacattc gtaacgctaa acgtctgaaa ggcgacaaac tgtgtctggt
    17161 taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac
    17221 aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt
    17281 aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt
    17341 gctacgtatg gcgacgctct atccggcgcg tgcgattggc gttgagaaac gtctcggcac
    17401 actcgccgca ggtaaagtag ccaacctgac tgcattcaca cctgatttta aaatcaccag
    17461 gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga
    17521 caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc
    17581 ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc
    17641 cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat taaagaagtt
    17701 gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat
    17761 ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca ccatcactct gtttgatctc
    17821 agtagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca gcagacgctg
    17881 gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg caagctgcgc
    17941 gagctgatcg ccatttccgt gatcctgcca gggcttgttg acccggacag cggcaaaatt
    18001 cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact ggaagaacgt
    18061 tttaaagtga cctgtttcgt cggtcacgat atccgtagtc tggcgctggc ggagcactac
    18121 ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtacatcg cggaaccggg
    18181 gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt
    18241 ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg
    18301 gaaactatcg ctgccaacgc cgctattgaa caacgggtgt tgaatctgtt aaagcagggc
    18361 taccagagcc gcgtgccgct ggacgactgc accataaaaa ctatctgcaa agctgcgaac
    18421 aaaggcgata gcctggcgtc ggaagtgatt gagtatgtcg gtcgtcatct gggcaaaacc
    18481 atcgccattg ccatcaacct gtttaacccg caaaaaattg ttattgccgg agaaatcacc
    18541 gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca ggcgctgaag
    18601 gcgtttcgca ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc
    18661 gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa
    18721 aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca
    18781 ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg
    18841 gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca
    18901 actatccttc gcagaccgga caagatctgg cgaaccgctt tgccacagcc ggtgtcgatg
    18961 tgccggacag cgtgttttat acctccgcga tggcaaccgc cgatttcctg cgtcgccagg
    19021 aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagcag
    19081 gtttcactat taccgatgtg aaccctgatt ttgtgattgt tggcgaaacg cgttcctaca
    19141 actgggacat gatgcataaa gcagcctatt tcgtcgctaa cggtgcacgc tttatcgcca
    19201 ccaacccgga cacccacgga cgcggttttt atccggcctg tggcgcgcta tgcgcgggga
    19261 tcgaaaaaat ctccggacgt aaaccgttct atgttggtaa gccgagtccg tggatcatcc
    19321 gcgcagcatt aaacaaaatg caggcgcatt cggaagaaac ggtgattgtc ggcgataacc
    19381 tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg gtactctccg
    19441 gcgtttcgtc gctcgacgat atcgacagta tgccattccg ccccagctgg atttacccat
    19501 cggtcgctga aatcgacgtt atctgaatag ca
//
LOCUS       DAOTRK010000088        19493 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.088, whole genome shotgun sequence.
ACCESSION   DAOTRK010000088 DAOTRK010000000
VERSION     DAOTRK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.088"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..408
                     /locus_tag="QIE41_002977"
     CDS             <1..408
                     /locus_tag="QIE41_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDS3531845.1"
                     /translation="DQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVE
                     YYFEPVTIQDSGTSILKNDLENSEDGAGQIGFQLSNDGTHEMQYGKSNYYSFQHPHEG
                     SNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(446..1345)
                     /gene="nhaR"
                     /locus_tag="QIE41_002978"
     CDS             complement(446..1345)
                     /gene="nhaR"
                     /locus_tag="QIE41_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDS3531846.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(1411..2577)
                     /gene="nhaA"
                     /locus_tag="QIE41_002979"
     CDS             complement(1411..2577)
                     /gene="nhaA"
                     /locus_tag="QIE41_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDS3531847.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3163..3315
                     /gene="mokC"
                     /locus_tag="QIE41_002980"
     CDS             3163..3315
                     /gene="mokC"
                     /locus_tag="QIE41_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDS3531848.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3419..4549)
                     /gene="dnaJ"
                     /locus_tag="QIE41_002981"
     CDS             complement(3419..4549)
                     /gene="dnaJ"
                     /locus_tag="QIE41_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDS3531849.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQSDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4638..6554)
                     /gene="dnaK"
                     /locus_tag="QIE41_002982"
     CDS             complement(4638..6554)
                     /gene="dnaK"
                     /locus_tag="QIE41_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDS3531850.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6930..7334
                     /locus_tag="QIE41_002983"
     CDS             6930..7334
                     /locus_tag="QIE41_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDS3531851.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKVARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7360..8073
                     /gene="msyB"
                     /locus_tag="QIE41_002984"
     CDS             7360..8073
                     /gene="msyB"
                     /locus_tag="QIE41_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDS3531852.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8222..8788
                     /gene="satP"
                     /locus_tag="QIE41_002985"
     CDS             8222..8788
                     /gene="satP"
                     /locus_tag="QIE41_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDS3531853.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8823..9410)
                     /gene="mog"
                     /locus_tag="QIE41_002986"
     CDS             complement(8823..9410)
                     /gene="mog"
                     /locus_tag="QIE41_002986"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDS3531854.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(9525..10478)
                     /gene="tal"
                     /locus_tag="QIE41_002987"
     CDS             complement(9525..10478)
                     /gene="tal"
                     /locus_tag="QIE41_002987"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDS3531855.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            10757..12187
                     /locus_tag="QIE41_002988"
     CDS             10757..12187
                     /locus_tag="QIE41_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDS3531856.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMALIWVLT
                     SLVICVMNIGQLPHVIWSIFENAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            12257..13033
                     /gene="yaaA"
                     /locus_tag="QIE41_002989"
     CDS             12257..13033
                     /gene="yaaA"
                     /locus_tag="QIE41_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDS3531857.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(13073..13369)
                     /locus_tag="QIE41_002990"
     CDS             complement(13073..13369)
                     /locus_tag="QIE41_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDS3531858.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(13583..14869)
                     /gene="thrC"
                     /locus_tag="QIE41_002991"
     CDS             complement(13583..14869)
                     /gene="thrC"
                     /locus_tag="QIE41_002991"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDS3531859.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(14870..15802)
                     /gene="thrB"
                     /locus_tag="QIE41_002992"
     CDS             complement(14870..15802)
                     /gene="thrB"
                     /locus_tag="QIE41_002992"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDS3531860.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15804..18266)
                     /gene="thrA"
                     /locus_tag="QIE41_002993"
     CDS             complement(15804..18266)
                     /gene="thrA"
                     /locus_tag="QIE41_002993"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDS3531861.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18292..18411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18347..18412)
                     /gene="thrL"
                     /locus_tag="QIE41_002994"
     CDS             complement(18347..18412)
                     /gene="thrL"
                     /locus_tag="QIE41_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDS3531862.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18626..19312)
                     /locus_tag="QIE41_002995"
     CDS             complement(18626..19312)
                     /locus_tag="QIE41_002995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDS3531863.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         4728 a         5131 c         4722 g         4912 t
ORIGIN      
        1 gatcaatcca tcgacactgg acgatttgta ctgtctcgta tcaataatga aaattatcag
       61 tttcatcata taagatttga ctgtatccag ggtgagcaag gtgaattagt ttttgaacct
      121 agtgatgttg aatattactt tgagccagtg acgatacagg attcaggaac cagcatatta
      181 aaaaatgatt tagaaaacag tgaggacggt gcgggtcaaa taggctttca actctccaat
      241 gatggtacgc atgaaatgca gtatggtaaa agcaattact actcttttca gcatccccat
      301 gaaggaagta atcagattcc tctatttatt cgcccgcgta catatggaaa taatgtctca
      361 agcgggcaaa taatgagccg agtcaaaatc gtggtaatgt acaactaatc tatctccccc
      421 accttaactt tgggggagct gccgattaac gtgccgctgg gctaaaaagc gcagaataat
      481 ccgtattgca gattcgctgt accgccgggt gctgaatcat ccgctcagca aaaatagcat
      541 ggtactcttc catcacattt tcgacgcgac caatttctac gacagtttta tcggcataaa
      601 agtcatatgc ataaagcgtt ggggcaacga agattgcatt gtgcatcgca ccaaaagctt
      661 tcatcaacgc ggcatcatca aactcgccga ggatttctac gtttaatccc tgggagttaa
      721 accagttaag caatttgcgc cctaacattg aacgtcgccc aggaatcaaa agccgccgtt
      781 cttccagaca agccgggaac ggtttttccg gtggtggatt tgtacaccag aaactcacgc
      841 cacattcgcc aattctcacg gagaacagac cttcctgctg cgtagaatct atcggacagt
      901 cggaaatgat catatccagt ttatgctgac ttaattgctc cagcagcatt tcgtgggtgg
      961 attcgaagca gcgaagatga atcggctcgc cttctaccac tgcggcgtta agtacgctac
     1021 tgaccaggcg tttagaaagt gcatcagcca cgccaacgtc aaacaataaa ttggattctt
     1081 tgcgatagtt cacgatatcc agcatttcct ggcttaaggt gaacatttta tcggcatagc
     1141 gatagaccag ctctcccagc tcgctgggtt cgagaccacg tcccttgcgt ttaaataatt
     1201 tgccttgcag gcgctcttcc agcgcccgaa tctgtccggt aatggtttgt ggtgttaaat
     1261 aaagcgcctc cgctgcgcca accacggaac cttctttata gacatgccag aagtaataca
     1321 agtggttgta attgatatga gacatgctca tttctctccc tgataacaat gaaaagggag
     1381 ccgtttatgg ctccccggta aaccgtcctg tcaaactgat ggacgcaaac gaacgcgtaa
     1441 ccagctgtat ccaattaccg ccgaagagat agaaccgacc aggataccga gtttcgccca
     1501 gttaatcagt tctggatcta cgctaccaaa ggccaggctg gcaataaaga tagacatagt
     1561 aaaaccgata ccgcacagga tccccaccgc cataatttgc tgataagtcg ttccctcagg
     1621 cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac agactaatcc ccagcggttt
     1681 gccaatcagc aagccagcga tgatccccaa tggtagaatg gaggtcaagc catccagcgt
     1741 gacgccttgc agtgaaacgc cagcattagc aaatgcaaac agcggtaaaa tcagatacgc
     1801 cacccacggg tgcaacacat gctccagtcg cttcgctgga gaacgcccat gcttctcttt
     1861 caaaggaata aagaagccga caattacccc cgccagggtt gcgtgaaccc ccgatttcaa
     1921 caccgcagtc cacaacacca cgccaaccag aatataaacg cccgtgcggc gtacaccaca
     1981 cagattcaat accgcgagta ccgcaattgc tacagccgcg acaccaagag aggccatcga
     2041 taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt cgataatagc
     2101 cagagccatc aaaaagatct tcagtgctaa cggaacacga cttcccaaca gcgccagcac
     2161 accaagtgca aaagcaatgt cagtagccgc cgggatcgcc cacccttcgc gggtaatcgg
     2221 atcggcatag ttaaaggcca gatagagcaa tgccggcaca atcatcccac caatagcggc
     2281 gataactgga aatgcagcct ggcgtaagct ggctagcgat ccttgcatca gttcacgttt
     2341 aacttccaga ccgactaaca ggaaaaatac cgccatcagc gcgtcattta tccataacag
     2401 catgtttttg ttgatttcga gtgaaccaac ccggagctga accggcgtct ccagaaagtc
     2461 gtgataccat ccactggttg cgccgctgtt ggccataatc atcgccagga tagcggcaat
     2521 gataagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat gtttcacttt
     2581 ttatttctct ttcaggtgaa tagatcgttt ttactatctt accccgcacg aatcatcgtt
     2641 aaaatcagat tatagtgtaa gaataattcg ttttttacga accattgaag gatttgtgcc
     2701 atcagcgatg agatgagttt ttgacggctg taagcgctca cgagtgaccc taaaccctga
     2761 taatcatagc ttaaggtcag gatttctgag ggattaatta ggaaaagtaa taattaattt
     2821 gttgtcagtc ggcgatgttt gttaggccaa tacttcaaaa ataatccagt ttgtcgtcaa
     2881 taattgcgat gaagagataa attaaagtta aataattaaa tcattaaaaa attacgccat
     2941 aaaaatgcga ggcgtcttca ggattagaat cgataaaaca gaattggtta aagagagcga
     3001 tataatagcg gcgggtgctt gaggctgtct gtctcaggca ttagctgaac ggcagataga
     3061 gaaaagcccc gagtgatatt ttaccatcaa cccgaggctc cctatatgct gaacacatgt
     3121 agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca gcataaggcg
     3181 atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct ggtaacgaga
     3241 aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt tttcacggct
     3301 tacgaatccg agtaagagca acggcgggga gtgatccccg ccactttaca ggtgctcgca
     3361 tatcttcaac gcaccctatt tttaccctgg cctgcccaca ggcaggcttt tggggaggtt
     3421 agcgagtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt gaacgcgggc
     3481 tgttgtgctc accggttggg ccaccgaagc tttcctgcag ctcttgcagc agctgtttct
     3541 gcttctcgtt cagacctacc ggtgtttcga cgacaacgcg gcacagcaag tcaccttgcg
     3601 caccgccgcg aacagacttg acgcctttac cgcgcatacg gaacagctta ccggtctggg
     3661 tttcgccagg cactttcagt ttgacgcgac catcaagggt cggtacttcg atttcgccac
     3721 ccagcgctgc catagcgaag ttgatcggga cttcgcaata caggttgtta ccttcacgct
     3781 cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc ggtgcgccat
     3841 gctcgcccgc ttcaccttca cccgcaagac ggatgcggtc tccagtgtcc actcctgccg
     3901 ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga catttgttgc
     3961 acggatcttt gatcagcgta ccgcggccct gacagtgtgg acaggtctgc tgcacggcaa
     4021 agaaaccctg gcgcatctgc acctgaccag aaccatgaca ggtcggacag gtctgcggct
     4081 gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga gtcggaatgc
     4141 ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg ttatagcgta
     4201 aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata tcgccgaaaa
     4261 cgtcaccaaa aatatcgctg aagtctgcgc cgccgccgaa accgccgccg cccatgccac
     4321 cttgctcaaa cgcagcatga ccatactgat cgtatgccgc acgtttttgc gagtcggtca
     4381 gaacttcata agcttccttg atctctttaa atttcgcctc ggcctctttg tcactctggt
     4441 tacggtccgg gtggtatttc atggccaggc gtttgtaggc ctttttgatt tcacgctctt
     4501 ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg cttagccatc ttttttaaat
     4561 tgcccctaga tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt cagtataatt
     4621 acccgtttat agggcgatta ttttttgtct ttgacttctt caaattcagc gtcgacaaca
     4681 tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg ctgggcatgt
     4741 tgctgctggg cgatttccat cagtttctgg gaaacctgtg ccagttcctg cattttcgct
     4801 tcgatagcgg ctttgtcttc acctttcaga gcagtttcca gtgcagtcag tgcagactcg
     4861 atagcagttt tgtcgtcagc cggcagtttg tcgcccgctt cttcaacctg cttacgggtg
     4921 ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa cttacggtca
     4981 gcttcggcgt tagcttctgc gtcgcgtacc attttctgga tttcatcttc gttcagacca
     5041 gaagaagcct tgatggtgat cttctgctct ttaccgctgt ttttatcttt cgcggaaacg
     5101 tgcaggatac cgtcagcatc gatatcgaag gtaacttcga tctgcggcat gccgcgcggt
     5161 gccgggttga taccatccag gttgaactga cccagagatt tgttatcagc tgcacgttta
     5221 cgttcaccct gcagcacatg gatggttacc gcagactggt tgtcttcagc ggtagagaac
     5281 acctggctgt gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt catcacaccg
     5341 cccatggttt cgatacccag agacagcggg gtaacgtcca gcagcagtac gtcttttacg
     5401 tcaccagtca gaacaccacc ctgaacagca gcaccgattg ctacagcttc gtccgggtta
     5461 acgtctttac gcggctcttt accaaagaac tcagcaactt tcttctgaac cattggcata
     5521 cgagtctgac caccaacgag gataacgtcg tcgatatcag atacggacag gccagcgtcc
     5581 tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca gatcttcaac caggctttcc
     5641 agtttcgcac gagtcacttt gatgttcatg tgtttcggac cggtcgcgtc tgcagtgatg
     5701 tacggcaggt taacgtcggt ctgctgagcg gaagacagtt cgattttcgc tttttctgcc
     5761 gcttctttca ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc ctgatctttc
     5821 ttgaattctt caaccaggta gttgatcaga cggctgtcga agtcttcacc acccaggtgg
     5881 gtatcaccgt tggtagccag aacttcgaag gttttttcgc cgtcaacttc gtcgatttcg
     5941 ataatagaaa tatcgaaagt accaccaccc aggtcataaa ccgcgatagt acggttgcca
     6001 gtacctttgt ccagaccgta agccagcgca gctgcggtcg gttcgttgat gatacgtttt
     6061 acttccagac cagcgatacg gcctgcgtct ttggttgcct gacgctgagc atcgttaaag
     6121 tatgccggta cggtgataac agcttcagtt accggttcac ccaggtaatc ttcagcggtt
     6181 ttcttcattt ttttcagcac ttcagcagaa atctgcggcg gtgccatttt ctggccttta
     6241 acttcgaccc atgcgtcgcc gttatcagca gcaataattt tgaacggcat gatggaaaca
     6301 tcacgctgta cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat cgcaaacagg
     6361 gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct gaccaaccag agtttcacca
     6421 tcctgggtat aggcaatgat agaaggcgtg gtgcgatcgc cttcggcgtt ctccagtaca
     6481 cgaggagtgg tgccatccat aatcgctaca caagagttgg tagtacccag gtcgatacca
     6541 attattttac ccatctaaac gtctccacta tatattcggt catcatgtgg ttgtgagtct
     6601 gtaatagggg cgaaacgtct ggtttcaact gcccaatttc atttttttgc agactcactc
     6661 actgcggttg actactaaat ggggtcgtaa cccacgtcat caagggggag atgcaaaaat
     6721 tttttgtgct tttgcgcaga aatttcgcga tatgccagaa caagacacga aactgacagc
     6781 aattttattc atatagagaa tgaatgacag ccaagacatt catagaaaaa ttgtaataaa
     6841 aagaaaatat tacgtacctg aatcttctct ttatcagcag taaactagtg ggtattcatc
     6901 ccctacctct ccccactaag agaatcctta tgaaatccgt ttttacgatt tccgccagcc
     6961 tggcgattag cctgatgctg tgctgcacgg cgcaggcaaa cgaccataaa atcctcggcg
     7021 tcattgcaat gccacgtaac gaaaccaacg atctggcgct gaaacttcct gtttgccgaa
     7081 ttgttaaacg catacaactc tcagccgacc atggcgattt gcagttaagc ggtgcatcgg
     7141 tttatttcaa agtcgcccgt agcgccagtc agagtctgaa tattccttca gaaataaaag
     7201 aagggcaaac cactgactgg atcaacatta acagcgataa cgacaataaa cgctgcgtct
     7261 caaaaatcac cttttccggt catacggtta actcatcgga tatggccacg ctgaaaatta
     7321 tcggcgacga ctaacgccag aatatagaag ccacaaaaaa tgaatgttaa ttacctgaat
     7381 gattcagatc tggattttct ccagcattgt agtgaggaac aattggcaaa tttcgcccgg
     7441 ctgctcaccc ataatgaaaa aggcaaaact cgcctctcca gcgtactgat gcgcaacgaa
     7501 ctgtttaaat cgatggaagg acatcccgag caacatcgcc gcaactggca gctgattgcc
     7561 ggagaattac aacattttgg tggcgatagt atcgccaaca aactgcgtgg acacggtaaa
     7621 ttgtatcggg ccattttgct cgatgtttca aagcgattga agctgaaagc cgacaaagag
     7681 atgtctacgt ttgaaattga gcaacagtta ctggaacaat ttctgcgtaa tacctggaag
     7741 aaaatggacg aggaacataa gcaggagttt ctgcacgcag tcgatgccag ggtgaatgag
     7801 ctggaagagc tgctgccgct gctgatgaaa gacaaattat tggcaaaagg cgtgtcgcat
     7861 ttgctttcca gccaattgac ccgcatttta cgcacccacg cagcaatgag cgtacttggg
     7921 catggtttgc tgcgcggcgc ggggctggga ggccctgtag gcgcggcact aaatggggtt
     7981 aaagcggtca gcggcagcgc ctatcgcgtg acgattccag ccgtactgca aatcgcctgc
     8041 ctgcgccgga tggttagtgc cactcaggtc tgataccgga aaccggggaa aattttcatt
     8101 ttcccctcaa aagatcgtag acactgcccc actggctgat tattatgccg cgccctgaaa
     8161 acactacagt tattcaggga aattatttca ccattcattc gatgatgatt tttgaggaat
     8221 tatgggcaac actaagttgg ctaatccggc accgctgggc ctgatgggct tcggcatgac
     8281 caccattctg cttaacctgc acaacgtggg ttatttcgct ctggacggta ttattcttgc
     8341 catgggcatt ttctacggcg gcatcgcgca aatttttgct ggtctgctgg agtacaaaaa
     8401 aggcaacact ttcggtttaa ccgcattcac ctcttacggt tctttctggc tgacgctggt
     8461 tgcgattctg ctgatgccga aactgggtct gaccgatgcg ccaaatgcac agttccttgg
     8521 tgtctacctg ggtctgtggg gcgtatttac gctgtttatg ttcttcggca cgctgaaagg
     8581 cgcacgcgtt ctgcaattcg ttttctttag cctgaccgtg ctgtttgccc tgctggcgat
     8641 cggtaacatt gccggtaacg ccgcgatcat ccactttgcc ggctggattg gtctgatctg
     8701 tggtgccagc gcaatctatc tggcgatggg tgaagtgctg aacgagcagt ttggtcgcac
     8761 cgttctgccg attggtgaat cgcactaatt gttttgagat ccccccgctc ggggggattt
     8821 ttttattcgc taacttcgcg tcttgcactc ttcggtctga atgcagcaac cacttccggt
     8881 gccgtttcaa cgtatggccc ttccagcaac tgaatgcagt acggtacgct ggcaaaaata
     8941 ccgtgcacca caacgttacc ctcagcgtcc tttacacctt ccagcgtctc tttaatcgac
     9001 ttcggctgac cgggtaagtt aaggatcagc gcctgtttgc gaatcacacc cacctgacgc
     9061 gaaaggatcg cagttggtac aaaatgcagg ctgatctggc gcatctgttc accaaagcct
     9121 ggcatctcgc ggtccgctac tgccagcgtc gcatcgggcg ttacgtcacg acgcgccggg
     9181 ccagttccgc ccgtggtgag caccagatgg caactcattt catccaccag ctcacacaac
     9241 gtttgctcga tgatcgcctg ctcgtcgggg attaagcggg tttccagttc aaacggcgtg
     9301 gttagcgccg atgtcagcca ttcttccaac gcagggatgc ctttatcctg ataaacgccg
     9361 ctggatgcgc gatcagagat ggaaactaag ccaatacgta aagtattcat caagattttt
     9421 ccgtttaaac agtctcgtta aacagaatga tacactgcga agggagtgac agacaggctt
     9481 cagaagaggt agcgtgacca acttcccggt cacgctaaga atgattacag cagatcgccg
     9541 atcatttttt ccagtttttc ctggtcaaca gcaaacttac ggataccttc cgccagttta
     9601 tctactgcca ttggatcctg gttatgctgc cacaggaact cggactcagt gatacgcgcc
     9661 ggacgcgctt tcacttcgcc ggtgtaagac agtttacgtt cgatagcccc ttcgctctca
     9721 gccagctctt tcagcagtgc cggtgcgatg gtcagacggt cgcagcctgc cagttccaga
     9781 atttcgccga tgttacggaa gcttgcgccc atcaccacgg tttcataacc gtgctctttg
     9841 tagtactggt agatttcaga tacagaaacc acgcccggat cttctgccgg agcgtactct
     9901 ttcttgtcgg tattcgcttt gtaccagtca agaatacggc caacaaacgg cgagatcagg
     9961 aacacgcccg cttccgcaca agcacgagcc tgagcgaagg agaacagcag tgtcaggtta
    10021 cagttaatac cttctttttc cagctgttct gcagcacgga taccctgcca ggtagaagcc
    10081 agtttgatca gaatacgatc gttgctaatc cctgcatcgt tgtagagttt gatcaggcgt
    10141 tttgctttcg caattgacgc ttcggtgtca taggaaagac gcgcatcaac ttcagttgag
    10201 atacggcccg gaaccagttt caggatttcc agaccaatat ttactgccag tttgtcggtc
    10261 gcgtccacga tctgctgcgc gcgatcgttg ctctgctgtt tcgcccaggc gacagcatca
    10321 tcaatcaact tacggtattc cggaatctgc gctgcgttaa gaatgagaga agggttggtt
    10381 gtggcatcct gcggttgata cagcttcatt gccgcgatgt ccccagtgtc ggccactacg
    10441 gtggtgtact gacgaaggga ggtcaatttg tccgtcatga tagtatttct ctttaaacag
    10501 cttgttaggg ggatgtaacc ggtctgccct gatgatatca cgacgcaacg tcagcgcaac
    10561 cgccgacaag acggttatcg ccaggcgaga ctgtttcgga tttctgacac tgcgttgata
    10621 tcgcccgcca tttttataca aaacctcatg tatgctacgc agaagttatc aagtacctcg
    10681 tagcgtatat acttcttaaa caattggtaa cgtttacaca ggaaagtcat cgcgaccggc
    10741 aataagaggg atatgcatgc cagatttttt ctcctttatt aacagcgtcc tttggggatc
    10801 ggtaatgatt tacctgctct tcggcgcagg ttgttggttc acttttcgca ccggatttgt
    10861 gcagtttcgc tacatccgcc agtttggcaa aagtcttaaa aatagcattc atccacagcc
    10921 aggcggttta acctcatttc agtcattgtg taccagtctt gcggcacgcg tgggtagcgg
    10981 caacctggcc ggcgttgcgc tggctattac cgccggtgga cctggagccg tcttctggat
    11041 gtgggttgcc gcgtttatcg gcatggcgac ctcgtttgcc gaatgttccc ttgcacaact
    11101 ttataaagaa cgtgacgtca atgggcagtt tcgtggcgga ccggcatggt atatggcgcg
    11161 cgggctgggg atgcgttgga tgggcgttct gttcgccgtc tttttgctca tcgcctacgg
    11221 cataattttc agcggagttc aggcgaacgc cgttgcccgc gccctaagtt tttcttttga
    11281 ttttcctccg ctggtgacag gcattattct tgctgtcttt gctctgctgg caatcactcg
    11341 cggtcttcat ggcgtcgccc ggctcatgca ggggtttgtc ccgttgatgg cgttaatctg
    11401 ggtactgacc agcctggtaa tttgcgtaat gaatatcggg caacttcccc acgtcatttg
    11461 gtctattttt gagaatgctt ttggctggca ggaagcggca ggcggcgcgg cgggatatac
    11521 cttaagccag gcgattacta acggttttca gcgcagtatg ttttccaatg aggcgggaat
    11581 ggggtcgacg ccaaacgcgg cagcggcggc tgcgtcctgg cctccgcatc ccgcagcgca
    11641 aggaattgtc cagatgattg gcatttttat cgacaccctg gtcatctgta cggcaagcgc
    11701 catgctgata ttactggcgg gtaacggcac aacctacatg ccactggaag gtattcagct
    11761 tatccagaag gcgatgcggg tgttaatggg ttcctggggt gcggagtttg ttaccctcgt
    11821 ggttattctg tttgccttca gctccatcgt tgccaactac atttatgccg aaaacaatct
    11881 cttcttctta cgcctgaaca accctaaagc gatctggtgt ctgcgaatct gcaccttcgc
    11941 aacggtcatc ggcggcacct tgctaagtct tccgctgatg tggcaactgg cagatatcat
    12001 aatggcctgc atggctatta ccaatttgac cgccatatta ctgctctcgc ctgtggttca
    12061 taccattgcc agtgattatc tacgccagcg taaactcggc gtgcgcccgg tgtttgatcc
    12121 gttgcgttat ccggatatcg gtcgccagct ttctccggac gcgtgggatg atgtttcgca
    12181 ggagtaatca caactatcga tcaactcatt ctcttttttt gctaaagtcg gcataaattt
    12241 cctgcaagga ctggatatgc tgattcttat ttcacctgcg aaaacgcttg attaccaaag
    12301 cccgttgacc accacgcgct atacgctgcc ggagctgtta gacaattccc agcagttgat
    12361 ccatgaagcg cggaaactga cgcctccgca gattagcacg ctgatgcgca tcagcgacaa
    12421 actggcgggt atcaacgccg ctcgctttca tgactggcag ccagatttca cgccggagaa
    12481 tgcccgccag gcgattctgg catttaaagg tgatgtctac accggcttgc aggccgaaac
    12541 cttcagcgaa gacgatttcg attttgccca acagcatttg cgaatgcttt ccggcttgta
    12601 tggcgtactg cgcccgctcg atttaatgca gccttatcgt ctggaaatgg ggatccgtct
    12661 tgagaatgcc cgagggaaag atctgtatca attctgggga gatatcatca ccaacaagct
    12721 gaacgaggcg ctcgcagcac aaggcgataa tgtggtgatt aatctggcgt cagatgagta
    12781 ttttaaatcg gtgaagccga agaaattgaa tgccgagatt atcaagccgg tgttcctcga
    12841 tgagaagaac ggcaaattta agatcatcag cttctacgct aagaaagcac gcggtctgat
    12901 gagtcgtttc attattgaaa atcggctgac caaaccagag caactgactg gttttaatag
    12961 cgaaggttac ttctttgatg aagcttcctc cagcaatggt gaactggtgt ttaaacgcta
    13021 cgagcagcgt taagtttgta gtcaatgccg gatggcaatc cggcattgat atttagcgat
    13081 gatgtttgtc aggccccgga cgatgatcgc cacgatgatc attgtgcttg ttatggcgcg
    13141 gcgatggcgg cggtccatat gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13201 accagccgtg gtcgcgccag tgaccgccat cccagtaata gccacgatta tcacgatcgc
    13261 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgcgct gccatgggag
    13321 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13381 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac ggcggttgtt
    13441 attctccgca atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct
    13501 gtcgtttctc tccatatttt cttcacaaaa aagccgggct gcataaaagc aaacccggcc
    13561 tgattgagat aatgaataga tattactgat gattcatcat caatttacgc aacgcagcaa
    13621 aatcggcggg cagattatgc gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13681 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13741 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgataag
    13801 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13861 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13921 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    13981 acgtcgcctg agtcgctttg ggggaccact gaccgtcgtg caggaaacgt ggcacggtat
    14041 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca
    14101 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14161 cttcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14221 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagctct tcatcatcaa
    14281 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcaa
    14341 tattgccgcc caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14401 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg
    14461 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca
    14521 tttgtgccat aaagcgaccg ccgaaatctt taaatgcaag cgttggcccg tgaaacaact
    14581 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14641 gtacgcgctc ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct
    14701 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14761 ccggcaggtc gtgcgggaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14821 gcgcaaagct gacctgctca ttgtgatctt taagattgta gagtttcatt tagttttcca
    14881 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    14941 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag
    15001 cgaacaaagt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15061 cctgccgcgc ctgccggaag cctggcagta accgttcacg gtagggttca gcgataacat
    15121 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga ataaagcctg
    15181 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15241 gggcttctgc cgtcgagact ttaatccccg gatacgccag cacccacagc cactcatcaa
    15301 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15361 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacggcct tccagctcgc
    15421 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    15481 tcagcgccgc gaccactgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15541 tttccagggt catcgccact ggaatttgct tacccagctc ctggcaaaaa cgctcccaac
    15601 actgataaac gatattttcc cgaggttctg acggcagctt atcggcaaag cgtccgaggt
    15661 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15721 caacaggtgt caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15781 caggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15841 tcggcaaaga caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15901 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    15961 aacagcggat cattaccatc cacttcggca atcttcacgc ggcagacgcc atcttcatca
    16021 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16081 aagagatcat cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16141 aactcagcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16201 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16261 tccggttcgg tataacccat ttcccgcgcc agagtagtcg cctcggagaa actcatgcct
    16321 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcatc
    16381 aattcatcac cagcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16441 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcatgacg caacagatgg
    16501 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16561 cgcaagaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16621 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16681 ttggcttgcg ccagttcttc ctgccagttt tccagattta ggccatgcac attggtgagc
    16741 agtgccttcg agttggcaac accgcagaca cgtaagtcga tatgtttatt cttcagccag
    16801 ctttgttgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16861 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16921 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    16981 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17041 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17101 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc
    17161 cgcacacagt cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17221 atcagcacca cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg
    17281 accattcctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17341 atgcccttga ccggtaattc gtcttcatca cgactggcac caatgagcgt acctggtgct
    17401 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17461 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17521 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17581 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17641 tagtcggaac cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag
    17701 cctgccatca gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac
    17761 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga
    17821 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17881 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    17941 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18001 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18061 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    18121 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18181 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18241 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18301 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18361 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18421 tgtactctgt aatttttatc tgtctgtgcg ctattcctat attggttaaa gtatttagtg
    18481 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18541 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18601 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18661 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgt tgtaaccagt
    18721 cgaccagttt tatgtcatct gccactgcca gagtcgtcag cagtgccatg gctcgttcgc
    18781 gtaaagcttg cagttgatgt ttgtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18841 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18901 ccgccaccat cggcacacca gtaagaacgt cagccaacgc taactcttcg ttagtcaacc
    18961 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19021 acagcggcac cagttctact ggcgtagcgt agtaatgata tttcgcccga ctgcgcgcag
    19081 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19141 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgcg
    19201 cctgactatc gacaatccgc agttcgctaa accccatcgt tttcattgcc cgcgccgctg
    19261 ccccaatatt ttctgctctg gcgggtgcga ccagaataat cgttatacgc atattgccac
    19321 tcttcttgat caaataaccg cgaaccgagt gatcactgtc aacttattac gcggtgcgaa
    19381 tttacaaact cttaacgtaa gtcgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19441 actatttcct gactgtacta acggttgaat tgttaaaaaa tgctacatat cct
//
LOCUS       DAOTRK010000089        19352 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.089, whole genome shotgun sequence.
ACCESSION   DAOTRK010000089 DAOTRK010000000
VERSION     DAOTRK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.089"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(77..2749)
                     /gene="infB"
                     /locus_tag="QIE41_002996"
     CDS             complement(77..2749)
                     /gene="infB"
                     /locus_tag="QIE41_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDS3531864.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(2774..4261)
                     /gene="nusA"
                     /locus_tag="QIE41_002997"
     CDS             complement(2774..4261)
                     /gene="nusA"
                     /locus_tag="QIE41_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDS3531865.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(4289..4741)
                     /gene="rimP"
                     /locus_tag="QIE41_002998"
     CDS             complement(4289..4741)
                     /gene="rimP"
                     /locus_tag="QIE41_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDS3531866.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(4948..5024)
                     /locus_tag="QIE41_002999"
     tRNA            complement(4948..5024)
                     /locus_tag="QIE41_002999"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4988..4990),aa:Met,seq:cat)
     gene            5372..6715
                     /gene="argG"
                     /locus_tag="QIE41_003000"
     CDS             5372..6715
                     /gene="argG"
                     /locus_tag="QIE41_003000"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDS3531867.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(6723..8348)
                     /locus_tag="QIE41_003001"
     CDS             complement(6723..8348)
                     /locus_tag="QIE41_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDS3531868.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQIFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(8799..8885)
                     /locus_tag="QIE41_003002"
     tRNA            complement(8799..8885)
                     /locus_tag="QIE41_003002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8849..8851),aa:Leu,seq:gag)
     gene            complement(8900..9232)
                     /gene="secG"
                     /locus_tag="QIE41_003003"
     CDS             complement(8900..9232)
                     /gene="secG"
                     /locus_tag="QIE41_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDS3531869.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(9460..10797)
                     /gene="glmM"
                     /locus_tag="QIE41_003004"
     CDS             complement(9460..10797)
                     /gene="glmM"
                     /locus_tag="QIE41_003004"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDS3531870.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(10790..11638)
                     /gene="folP"
                     /locus_tag="QIE41_003005"
     CDS             complement(10790..11638)
                     /gene="folP"
                     /locus_tag="QIE41_003005"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDS3531871.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(11728..13662)
                     /gene="ftsH"
                     /locus_tag="QIE41_003006"
     CDS             complement(11728..13662)
                     /gene="ftsH"
                     /locus_tag="QIE41_003006"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDS3531872.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(13762..14391)
                     /gene="rlmE"
                     /locus_tag="QIE41_003007"
     CDS             complement(13762..14391)
                     /gene="rlmE"
                     /locus_tag="QIE41_003007"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDS3531873.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            14517..14810
                     /gene="yhbY"
                     /locus_tag="QIE41_003008"
     CDS             14517..14810
                     /gene="yhbY"
                     /locus_tag="QIE41_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDS3531874.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(14966..15442)
                     /gene="greA"
                     /locus_tag="QIE41_003009"
     CDS             complement(14966..15442)
                     /gene="greA"
                     /locus_tag="QIE41_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDS3531875.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            15690..17123
                     /gene="dacB"
                     /locus_tag="QIE41_003010"
     CDS             15690..17123
                     /gene="dacB"
                     /locus_tag="QIE41_003010"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDS3531876.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNDPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(17163..18335)
                     /gene="cgtA"
                     /locus_tag="QIE41_003011"
     CDS             complement(17163..18335)
                     /gene="cgtA"
                     /locus_tag="QIE41_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDS3531877.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(18351..19316)
                     /locus_tag="QIE41_003012"
     CDS             complement(18351..19316)
                     /locus_tag="QIE41_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDS3531878.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
BASE COUNT         4700 a         5301 c         4544 g         4807 t
ORIGIN      
        1 acgcgcgagc gtcatatcag gccacggctg ccggatgcgg cgtaaaagcc ttatccggcc
       61 tacaacccag acaaccttaa gcgatggtac gttggatttc gatgatctcg aatacttcga
      121 tcacatcgcc agtgcggaca tcgttgtagt tcttaacgcc gataccacat tccataccgt
      181 tacggacttc gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga
      241 taaccacgtt gtcgcgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa
      301 ccatacagcc tgcgatggca ccaaatttcg gtgatttgaa cacgtcacga acttccgcca
      361 gaccgataat ctgttgtttc agttccggag acagcatacc gctcattgcc gctttcactt
      421 cgtcgatcag gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatta
      481 ctttacgtgc agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg
      541 cagccagggt agcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt
      601 taacttcgtc agtagacagt ttcagcaagg agtcggagat agcttccaca gaaccctgta
      661 cgtctgcctt caggacgata ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca
      721 tgttctcgag tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac
      781 gatagagtgc aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg
      841 cagccggaac gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca
      901 cttcctgacc cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa
      961 cgatatcgcc cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac
     1021 ctttatcgag gaaggattcg ataaccgcac cgctcgccat gcctttacgt accgctttca
     1081 gttccagaac ttccgcctgc agcaggatag cgtccagcag ctcatcgata ccagtacccg
     1141 ctttcgcaga cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt
     1201 actgggagag ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca
     1261 ctgcaacgac caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atggtctgcg
     1321 gcattacgcc gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac
     1381 gagcacgcat tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt
     1441 tttcagtttc aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg
     1501 ccactttcgt tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca
     1561 tgatggtcac aaccggcgcg cgcggttcag ccgctgcacc cgtgtcacgg tcgctcatta
     1621 ccgcttcttc cagctcgttt tcacgacgca ggataacttt gtggcccatc tcttcggcaa
     1681 ccagctgtgc ggtttcctgg tcgatgacct ggttgatggt cgccattgcg cccagcttca
     1741 tcatcgcttt gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg
     1801 tgatggtttc gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt
     1861 gctgcagcga agaaccttta cgttttccgc ccttaccgcc acgtactgct gcgcgtgctt
     1921 cttcacgatc agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gcagctttcg
     1981 cgttacgacc acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc
     2041 gagcatgttg agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat
     2101 cagtccattt gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt
     2161 cttcgagttt acgacgcgct tcttcttcgg ctttacgctt gagctctgca gcttcctgct
     2221 cacggcgggc tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttactcactt
     2281 tgtctttttc cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg
     2341 cggcctcacg ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac
     2401 gggcttgctc ttccgcttca cgctgcgctt gctcttccgc agcaaggcgt tcagcctctt
     2461 gcggatcgcg tttcacaaag gtgcgtttct tgcggacttc gatttgtacc gatttgcttt
     2521 ttccaccggt accaggaatg ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt
     2581 ccgggcctga atttttctga ttcaggtggt caatcaaagt ctgtttctct tgtgcggaca
     2641 cagagtcgtc agcagacttc cggatacctg catcagcaaa ttgctgtacc aggcgttcca
     2701 cggaggtctg tcgctctgcg gccagcgttt taatcgttac atctgtcatg ctgttccttc
     2761 ctgctacagt ttattacgct tcgtcaccga accagcaaat attacgggca gccataatca
     2821 gtgctccggc tttttcgtcg gtcaaccctt cgatatcagc cagatcatca atgccctgtt
     2881 cggcgagatc ttccagcgta caaacgccac gggcggccag tttgaatgcc aaatcacgat
     2941 ctatcccttc aaggttcagc agatcgtcag ccggtttgtt atcaccgagg ctttcttcct
     3001 gggcctgtgc aatggtggcc agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg
     3061 gctcatcaag gccttcgatt tccaacagct ctttcatcgg cacataggcc agttcttcca
     3121 gcgtcgagaa gccttcttct accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt
     3181 tggtgaaagt gtcgatcgct gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa
     3241 cggtcatcac gttgagttcc caaccgctca gctgcgaagc cagacgcacg ttctgaccat
     3301 tacggccaat cgcctgcgcc aggttaccgg cttcaacggc gatatccatg gtgtgtttat
     3361 cttcatccac cacgatagaa gcaacgtctg ccggtgccat tgcgttaatc acgaactgcg
     3421 ccgggttatc atcccacagg acgatatcga tacgctcgcc gcccagttca gtagaaaccg
     3481 cctgaacacg cgcgccacgc atacctacgc aagcacctac cggatcgata cgtttatcgt
     3541 tggttttcac cgcgattttc gcacgagaac ccggatcgcg agccgctgct ttaatttcaa
     3601 tcacttcttc gccgatttcc ggcacttcaa tgcggaacag ttcgatcagc atttccggct
     3661 tggaacgggt gacgaacagt tgcgcgccac gcgcttccgg gcgaacggaa tagagcacgc
     3721 cacgaacgcg gtcgccagga cggaagtttt cacgcggcag catatcttcg cgcaggatca
     3781 cggcttcagc gttgttgccc agatccagag agatgttgtc gcggtttact tttttcacca
     3841 cgccggtgat gatttcacct tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg
     3901 cttcacgcac tttctgcacg ataacctgtt ttgccgtctg ggtggtgata cggtcaaagg
     3961 taacagactc aatctgatct tcaacgtaat cgcccaggtt caggctttca tcttcataac
     4021 gtgcggcttc aagggtgatt tccttggtcg gctgggtgac ttcatcaaca actaaccagc
     4081 gacggaaggt gtcaaaatca ccgcttttgc gatcgatctg tacgcgaacg tcgatttctt
     4141 gttcatattt tttctttgtt gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct
     4201 cgcgaggtag cgccttttca ttggatacgg cttcaactac agccaaaatt tctttgttca
     4261 tcgcgggctt ttcacctcat ccagactatt aaaagtgggg aaccaggttc gccttctgga
     4321 tattactcag cgcgaacact tcatctttac cttcgacggt aactgtgatc atttcaccgt
     4381 ctaccgcttt gataacgccc tgccatttac gacggttttg taccgccatg cggagaacca
     4441 gagtcacctc ttctccgacg aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga
     4501 gacccggtga ggagacttcc aggttataag caacggtgat gggatcttca acatccagca
     4561 cagcacttac ctggtggctc acatcagcac aatcatcaac attgatgcca tcttcactat
     4621 caatatagat gcgcagtgtg gatgtgcgac cgcgaataaa ttcgatgcca accagttcaa
     4681 aacccagggc ctcaactggc gcagtaatca tctctgttaa tttttgctct aatgtggaca
     4741 agcccacccc caagacataa aaaaagggcc taaagcccag ttattctgta gtcagataac
     4801 aaaaaacccc gataaatcgg ggctttatat aactgaaccc tataaccgca actgcggtct
     4861 ggagcacttt ccagaaggat tttttcaaat cccactacga aggccgaagt cttcacagta
     4921 tatttgaaaa aggactctaa gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct
     4981 tcgggttatg agcccgacga gctaccaggc tgctccaccc cgcgcctgaa acgtggcaaa
     5041 ttctactcgt tttgggtaaa aaatgcaaat actgctggga tttggtgtac cgagacggga
     5101 cgtaaaatct gcaggcatta tagtgatcca tgccacattt tgtcaacgtt tattgctaat
     5161 cacgtgaatg aatatccggt tcactttcat ttgttgaata cttttgcctt ctcctgctct
     5221 cccttaagcg cattatttta caaaaaacac actaaactct tcctgtctcc gataaaagat
     5281 gattaaatga aaactcattt attttgcata aaaattcagt gagagtggaa atccaggctc
     5341 atcatcagtt aattaagcag ggtgttattt tatgacgacg attctcaagc atctcccggt
     5401 aggtcaacgt attggtatcg ctttttccgg cggtctggac accagtgccg cactgctgtg
     5461 gatgcgacaa aagggagcgg ttccttatgc atatactgca aacctgggcc agccagacga
     5521 agaggattat gatgcgatcc ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct
     5581 gatcgactgc cgcaaacaac tggtggccga aggtattgcc gctattcagt gtggcgcatt
     5641 tcataacacc accggcggcc tgacctattt caacacgacg ccgctgggcc gcgccgtgac
     5701 tggtaccatg ctggttgctg ccatgaaaga agatggcgtg aatatctggg gtgacggtag
     5761 cacctataaa ggaaacgata tcgaacgttt ctatcgttac ggtctgctga ccaatgctga
     5821 actgcagatt tacaaaccgt ggcttgatac tgactttatt gatgaactgg gcggccgtca
     5881 tgagatgtct gaatttatga ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc
     5941 ctactccacg gactccaaca tgcttggtgc aacgcatgaa gcgaaggatc tggaatacct
     6001 caactccagc gtcaaaatcg tcaatccaat tatgggcgtg aagttttggg atgagagcgt
     6061 gaaaatcccg gcagaagaag tcacagtacg ctttgagcaa ggtcatccgg tggcgctgaa
     6121 cggtaaaacc tttagcgacg acgtagaaat gatgctggaa gctaaccgca tcggcggtcg
     6181 tcacggcctg ggcatgagcg accagattga aaaccgtatc atcgaagcaa aaagccgcgg
     6241 catttatgaa gcccctggga tggcactgct gcacattgcg tatgaacgtt tgttgaccgg
     6301 tattcacaac gaagacacca ttgagcagta tcacgcgcat ggtcgtcagt tgggccgtct
     6361 gctgtaccag ggacgttggt ttgactccca ggcgctgatg ctgcgggact ctctgcaacg
     6421 ctgggttgcc agccagatca ctggtgaagt tactctggag ctgcgccgcg ggaacgatta
     6481 ttcaatcctg aataccgtct cagagaacct gacctacaag ccagagcgtc tgacgatgga
     6541 aaaaggcgac tcggtgttct cgccagatga tcgtatcggt caattgacca tgcgtaacct
     6601 ggatatcacc gatacccgcg agaaactttt cggttatgcc aaaactggcc tcctctcctc
     6661 ctctgccgct tcaggcgtgc cgcaggtgga gaatctggaa aataaaggcc agtaattcga
     6721 catcaaccct gcccttcagg ggtggggtta ccttgcttat cctctgtaaa ctcctttctg
     6781 agcatcacat aatcgaaaac atcatggttt gcatccacaa cccgtgagtc tcctttatag
     6841 tgtgcaatca cttcctccag cgttttcggc tgttccggct ttgttacccc catccacgca
     6901 ttaataaggt aattattgta cgcagtggaa aagatatcgt tttccgttgt acgatcaacg
     6961 ccatccccaa gaacggggct ataccagata aacatcggga catgatatgc ctgctggctg
     7021 gcctccctgc ctccatgaaa atagacgttc ttcttcgtag ggtcgcgttc caggccgtgg
     7081 tcggcaaaat acatgaccga ggcgcggcga tcttttaata attcaaaaat ctgacctagc
     7141 aaactatctg tgtaatgaat ggagttgtca tagcaggcat cctgatcgtc ctgcggttga
     7201 aacacggcgc tggattgcgg ataggcgcta caagccggtt catggcttcc atttaaatga
     7261 agaacaatca gtttcttttg ctgcgtcttt tgctgtaacg cttgcgataa atgcggcaac
     7321 aacaattcat caaatcctct gacatagact gtttccatgg cgcgcatggc gatactggta
     7381 actgctgtac cattctgccg aaaagcggat tgcgagctta gccagaaagt ctgaaatcct
     7441 gcttgattag ccatattaat aatgttgtcc gggtaattat gaatgtcatg actcaaaaca
     7501 gaatcagcag ttaaagaaag gggaaccgac agcgcagtgt aaggtgcacc gcttattgct
     7561 tgattaaaca gtttgatctg ttttctttgt gcttcgactt gcggtgtcgt agagcgtgtg
     7621 tatccataca aagacatatt gtcgacacgt acagactccc ccacaatcaa cacgtaggta
     7681 tcaatacctg tatccctgac tgataattga aaatatggca ccgtgtttgc aattgagagt
     7741 aatctttgat gctctttcgc tgctaaagca aaataattga gattgaaaaa cggcgtatag
     7801 gtagcaaatc gcgacgctag tatatatgga ctgaacgctt ttttattttt tgcatcttta
     7861 taagcaaatt gacaagcgga aaataaactg cccgaaatga caatcagcaa taatattcct
     7921 gtcacttttt tcgtcggcaa ggaaacatca tattttatta ttactgccaa aaaaagaagg
     7981 gataaaaagg caaaagcaca tagataaggg atatacatcc ccagcatttt gacaacttca
     8041 tccggatcac tctgcagcac actaatcgca aatccatcat tgaaagttgt accaaaggta
     8101 caccatgaat agaggctaat actcatgtct gccgctgtca gtaacacaaa tggtgcggca
     8161 acaagcaggc gaagcggaag ggatgatata cgtttaacaa ccaagatggt cagaacgaag
     8221 aacagcaatc gctgaaccat atgcgctccg gaagaggcga ctaagttcgt aataccaaaa
     8281 acaataacac aaagatacaa caaccaatga gatttaaaac ttctaacaaa tttgttgaat
     8341 actgtcatcc agaaatccaa gttcacaacc aatataaaca attatcaaca atgttcaatt
     8401 ttatatcaga catgtttttc aactctgatc catatcagac cgaaacactg ttagtttttt
     8461 ctttttaaat gatgcggcca ttgttctcag taattaaata acattatcaa tgcgtctcta
     8521 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
     8581 gaagtaaaca tatccgtacc ggaactggag ttgacacaat aaagtgccaa ttatgtcagc
     8641 agaagggaaa agtttataac taaggcgtac cggcaccatc gtttcaaagt accagctacg
     8701 agtaaagcaa ctggacgaga tacagatacc tgacaaccat tcctcagacc aggaccaaaa
     8761 cgaaaaaaga cgcttttcag cgtctctttt ctgaaatttg gtaccgagga cgggacttga
     8821 acccgtaagc cctattgggc actaccacct caaggtagcg tgtctaccaa ttccaccacc
     8881 tcggcacgga tactactttt tagttcggga tatcgctggt cggcttagcc ggagcagctg
     8941 gctgagtttg ttcggttttc gccggcgcac tcaggttttc ccattcgcta cctttattgg
     9001 ttttattgct attgatgtta cccagcacca gactgatgat gaagaataac gttgccagca
     9061 gcgccgtcat gcgggtcatg aagttaccag aaccacttga accaaacagc gtagcggaag
     9121 cgcctgctcc gaaggaggct cccatatcag cgcctttacc ttgctgcagc atgatcagac
     9181 caacaaggcc aattgccaca ataaggaaaa ctactaaaag agcttcgtac ataatcaacc
     9241 tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa taaagcggga gtttttaatc
     9301 tttcccactg aagcgggtgt gaatactaac caaagcgaat gaccttcgca agggcaattt
     9361 tatcgcattg tatcaactgc gggaaaaaac agcaaaaccg atgtgttcgc cgagaaaaaa
     9421 ggcgacaatc gccgcctttt tagccagtta tctaacgctt taaacggctt ttactgcatc
     9481 ggcgatacgg tgtgcaaatt cagttacctg cgcttcgtct tcaccttcta ccatcacgcg
     9541 aattaacggc tcggtgccag atttacgcag caaaacgcgt ccacggttgc ccagcgcagc
     9601 ttcaacctct gcggtcacgg ctttaactga ctcatgctca agcggatcgc cgctacctgc
     9661 ggtgtaacgc acgttaacca gaatctgcgg gaacattttc atgccgctgc aaaggtcgtg
     9721 caggctcata tggttacgcg ccatcgccgc cagcacctgc aagccagcaa cgataccgtc
     9781 accggtagtg gttttatcca gcaggatcac atgaccggag ttctctgcac cgatacgcca
     9841 gcctttctcc tgcatttttt ccagtacgta gcggtccccc actttcgcgc gcgcaaatgg
     9901 aatacctaac tgtttcagcg ccagttcaag ccccatgttg ctcatcaatg tacccaccgc
     9961 gccaccacgc agctggccct gacgaagacc ttcacgcgcg atgatataca tgatctgatc
    10021 gccatcgact ttattgcctt catggtcaac cataatcacg cgatcgccgt cgccgtcgaa
    10081 ggcaataccg agatccgctt tttcagccag cactcgcgcc tgaagcgcgc gaacgtcggt
    10141 agcccccact tcggcattga tgttcacacc gttcggctca caaccgatag cgataacatt
    10201 cgcccccagt tcgcgcagca cgttcggcgc gatgtgataa gtcgcaccat ttgcacaatc
    10261 caccacaatc ttcagttcac tgaggctaag ttcgttcggg aacgtggctt tgcaaaactc
    10321 gatatagcga cccgcagcat caacgatacg gctggcttta cccagttctg ccgaatcaac
    10381 acagctgatc tctttttcca tttccgcttc gatggcctct tctactgcat ccggtagttt
    10441 ggtgccgtcg atagagaaaa atttaatgcc gttatcgtag aacgggttat gcgatgcaga
    10501 tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga taagccacgg ccggtgttgg
    10561 catcgggcca gtgaagagcg cggaaaggcc cgctgccgcc agacccgctt ccagtgctga
    10621 ctccagcata tagccagaaa tacgcgtgtc tttaccaata atgatcttac gagagccgtg
    10681 gcgcgccagc actttacccg cggcccaacc cagcttaagc acaaaatcag gggtgatcgg
    10741 cgcatcccct acacgaccac gaatcccatc ggtaccgaaa tatttacgat tactcatagc
    10801 gtttgttttc ctttgcagac agagtggctt ccaccacccg catcgcttct acggtttctt
    10861 tgacgtcatg aacacgaatg atatgcgcgc cttgcatagc ggcaatgacc gcacaagcca
    10921 gactaccgct cagacgctcg gacggcccca cgttcagcag ctgcccaatc atcgattttc
    10981 gtgacatacc caccaacagc ggcaggttga aatggtgaaa ttcagccagg cgcgccagta
    11041 atgaatagtt atgggagaga tttttaccga aaccgaatcc ggggtcgagc aacaatttct
    11101 cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc aataaaatag cgattcactt
    11161 ccgcaaagac atcgtcatac ttcggggctt cctgcatggt ctttggattt ccctgcatat
    11221 gcatcaggca aaccggcaaa ccggtttctg cagcagcctc cagagcgcct ggttcggaaa
    11281 gggagcgaat atcgttaata atgtgagcgc caactttcgc tgactcacgg atgacttctg
    11341 gtttggatgt atcgaccgag atccagactt cgaagcgttg agcaattgcc tcaaccacag
    11401 gaataacacg ttgcaactct tcttcaacgc taacttccgc cgcccccggg cgtgtggact
    11461 cgccaccaac gtcaatgatc gtcgcgccag cgttgatcat cagattcgca tgtttcaccg
    11521 catctatcag cgagttatgc gtgccgccat ccgaaaagga atcaggcgtg acgttgagga
    11581 tccccattac gtgaggatgg ctaaggtcca gtgaagtgcc ctgggcaaag agtttcatgg
    11641 tgttatccct ggtataaatt gataagaaaa aaccccggag cacgccccgg ggttttcggt
    11701 acaaatacag tcatctgatg cgggaactta cttgtcgcct aactgctctg acatggtgtt
    11761 acccgggttc ggcgtacgcg gttcatcaac cggacgagga gcctttggac tgccattgtc
    11821 gccagaattg ttagaagcgc ctggttcttc ccagcccgct ggcggacgta catcgcgacg
    11881 tgccatcagg tcatcaatct gcggtgcgtc gatagtctca tatttcatga gagcatcttt
    11941 catcgcatgc agaatatcca tattgtcggt cagaagctga cgcgcacgat tatagttacg
    12001 ctcaatcagt gctttcactt cctggtcgat gatacgtgca gtttcatcag acatatgttt
    12061 cgctttcgct acgctacggc cgaggaacac ttcaccttct tcttccgcgt acagcagtgg
    12121 acccaatttc tcagagaagc cccactgagt caccatgtta cgtgccaggt tggtcgcaac
    12181 tttaatatcg ttggacgcac cggtagaaac atgttccggc ccgtagatga tctcttctgc
    12241 cagacgacca ccgtacagcg tagaaatctg gctttccagt ttctgacggc tggcgctgat
    12301 tgcgtcgccc tcaggcaaga agaaagtcac acccagcgca cgaccgcgtg ggataatcgt
    12361 cactttgtgc accggatcgt gttccggcac caggcgaccg ataatcgcat gacccgcttc
    12421 gtggtaagcc gtcgattctt tctgcgcttc cgtcatcacc atggagcgac gttccgcacc
    12481 catcatgatt ttgtctttcg ctttctcgaa ctcaaccatc gacacaacgc gtttgttgcc
    12541 acgagcagcg aacagtgccg cttcgttcac caggttcgcc aggtcagcac cggagaaacc
    12601 aggagtacca cgggcaatga ttgccgcgtc gatatcgggt gccaatggta cgcgacgcat
    12661 gtgaactttc aggatctgct cacgaccacg aacatctggc aagccaacca caacctgacg
    12721 gtcgaaacgg ccaggacgca gcagggccgg gtcaagaacg tccggacggt tagtcgcggc
    12781 gataacgatg ataccttcgt taccttcgaa gccatccatc tcaaccagca tctggttcag
    12841 agtctgttca cgttcatcgt gaccaccgcc cagaccagcg ccacgctggc ggcctacggc
    12901 gtcgatttca tcgataaaga tgatgcacgg tgccgctttc ttcgcctgtt cgaacatgtc
    12961 acgaacacgg gatgcaccca caccgacgaa catttcgacg aagtcagaac cggagatagt
    13021 aaagaacgga actttcgctt cgcctgcaat cgctttcgcc agcagcgttt taccggtacc
    13081 cggaggaccg accatcagga cgcctttcgg gatcttaccg ccgagtttct ggaagcggct
    13141 cggctcgcgc agatactcaa ccagttcagc aacttcttct tttgcttcgt cgcagcccgc
    13201 aacgtcagca aaggtcgttt tgatctgatc ttccgtcagc atgcgcgctt tgctcttacc
    13261 aaacgacatg gcacctttgc caccgccgcc ctgcatttga cgcatgaaga agatccagac
    13321 accaatcagc aacagcatcg ggaaccagga gatgaagata gaagccagca ggcttggttc
    13381 ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac aggttatcca gtaatttcgg
    13441 atcctgaacc ggaatgtaag tggtataacg gttactatct ttcttggtaa cgttgatttc
    13501 acgtccgttg atacgcgctt cacgaacctg gtcgttattg acctcttgta ggaaggtaga
    13561 gtaatccacc ttacggccat tagactcgct gggcccaaag ctctggaata ctgacatcag
    13621 cacaacggca atgaccagcc agagtattag gtttttcgcc atgtcactca agggattaac
    13681 ctcttattac aactgtgtta aaaacagcgt caggatactc tatatccagc atctttcaaa
    13741 ctttcgtctg aaatctcccg gttagggttt acgcccggtc gctacaatat acacttcccg
    13801 cgaacgtgcg cgagaagagt ccggcttacg aactttgacc ttcgtaaaca gggagcgaat
    13861 ttcccttaga tactcatcga aaccttcgcc ctggaacacc ttcactacaa aactgccacc
    13921 tggcgctaat acatcacgac acatttctag cgccagttcc accagataca tggcacgggg
    13981 gatatccacc gccggtgttc cgctcatgtt tggtgccata tcggacatga caacctggac
    14041 tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc agttcatcac gaaaatcgcc
    14101 ctgaagaaag tccacaccaa cgataggatc cataggtaaa agatcgcaag cgatgatgcg
    14161 gcctttgccg ccaatttggg tgaccacata ttgtgaccaa ccacccggcg cagcaccaag
    14221 gtcgacaacc gtcattcccg gtttaaagag tttgtcactt tgctgtattt catcaagttt
    14281 aaaccaggca cgggaacgta accccttttt ctgtgcctgt tgaacatatt tatcgctaaa
    14341 gtgttcctga agccagcggc tggagctggc agaacgcttc ttacctgtca tttaactttc
    14401 ccatggggat aactcatcgt aaccaattgc gtaaattttt acgcgcctat ttggtgatat
    14461 atgggagatg gcggtagaat gacccgtttt caatcccaac gtaagcaaaa aatacgatga
    14521 atctgagtac taaacaaaaa cagcacctga aaggtctggc acatccgctc aagccagttg
    14581 ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc cgagattgaa caagcgttag
    14641 agcaccatga actcatcaag gtgaaaatcg ccaccgaaga tcgcgaaact aaaaccttga
    14701 tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt acaggtcatc ggtaaaacgc
    14761 tggtgcttta tcgcccaact aaagaacgta aaatctcgct gccacgctaa gattatccta
    14821 aagttacaca catttgctgt gtaaaacgag gggttttccg caggcaggag agcaaaatgc
    14881 cacgctctgt tcgttgataa aaggccgcat agcggccttt ttcctttctt tacaatacat
    14941 caacatcttg agtattgggt aattcttaca ggtattccac cttaattact tcaaattcta
    15001 cttcgccgcc cggcgttttg atgaccacaa catcatcttc ttctttgccg atcaggccac
    15061 gagcaatagg cgagtttaca gaaatcaggt tttgtttaaa gtcagcttcg tcatcgccaa
    15121 cgatgcgata agtctgttct tcgtcagaat cgagattcag caccgttacg gtagcaccaa
    15181 aaataacgcg cccattgttg ggcattttgg tgacatcaat cacctgcgcg ttcgacagct
    15241 tggcttcgat gtctttgata cggccttcgc agaagccctg ctgttcacga gctgcgtggt
    15301 attcggcgtt ttctttcagg tcgccatgct cacgcgcttc cgcgatagca gcaatgattt
    15361 caggacggcg cacagatttc agaaaatcca gctcttcgcg taatttttca gcgccgcgta
    15421 aggtcatcgg aatagcttgc atttgttata cctcttgaat attcctgatg gggcaagtct
    15481 tcacccaccc cggctgttcg gcctgcccgg cataactcct caccgggacc agaagcaaaa
    15541 aaataccgac ccgggtacaa gtcccaggtc agctacaatt cacattttga tagtcatttt
    15601 accctgaagt tcccgaaggg tcatcgttta ctttataggg cgttgcgccg tagtatgacg
    15661 gctcgattcc aggttgttag cgcgagatta tgcgattttc cagatttatc atcggattga
    15721 ccagctgtat agcgttcagt gttcaggccg caaatgttga tgagtacatt actcaactcc
    15781 ccgctggtgc caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg
    15841 attaccacag tcagcagatg gcgctgcccg ccagtacgca gaaagttatt actgcgctgg
    15901 cggcgttgat tcaactcggc cccgattttc gttttaccac gacgcttgaa accaaaggta
    15961 atgtggaaaa cggcgtactt aagggtgact tagtggcgcg atttggtgcc gatccgacgt
    16021 taaaacgtca ggatattcgc aatatggtcg cgactttgaa aaaatctggc gtcaaccaaa
    16081 tcgatggcaa tgtgttgata gatacctcca ttttcgccag ccacgataaa gcccccggct
    16141 ggccatggaa tgacatgaca caatgcttta gcgctccgcc tgccgccgcc atagttgacc
    16201 gcaactgttt ctccgtctcg ctctacagtg ccccaaaacc tggtgatatg gcttttatac
    16261 gcgtggcatc ttattacccc gttacgatgt tcagccaggt acgcaccctc ccccgtggtt
    16321 ctgccgaagc gcaatattgc gagctggatg tggtgccagg cgacctgaac cgctttacgc
    16381 tgaccggatg cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg
    16441 gagccagcta tgccggtgca attctgaaag atgagttaaa acaggcaggt atcacctgga
    16501 gcggaacact gctgcgtcag acgcaggtta acgaccccgg aacggtagtt gccagtaaac
    16561 agtcggcccc gctgcacgat ctgcttaaga ttatgctgaa aaagtcggac aacatgattg
    16621 ccgatacggt tttccgcatg atcggccatg cgcgcttcaa tgtgcctgga acatggcggg
    16681 ccgggtcgga cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat attggtaaca
    16741 ccattattgc cgatggttca gggctttcgc ggcataacct gattgccccc gccaccatga
    16801 tgcaggtgct gcaatacata gcccaacacg acaatgaact taactttatc tctatgctgc
    16861 cgctggcggg ctatgacggc tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg
    16921 atggaaaagt ctcagcgaaa accggttcgt tgcagggggt atataacctg gcgggattca
    16981 ttaccacagc gagcgggcaa cgaatggcgt ttgtgcaata tctttctggc tatgcagtag
    17041 aaccggcgga tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata
    17101 aagatattta tcagaacaat tagtcaataa gaaaccccgg cgcatggccg gggttttgct
    17161 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    17221 cagtcttcat catcctcttc agcaatctct tcaagctgct ggcgatgata atcatcccac
    17281 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    17341 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccactcgc cgcagagatc
    17401 agataatatt tatcttccca gcccagcgcc tcagcgatcg ctttcgcttt ctcttcggct
    17461 tctaccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    17521 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    17581 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    17641 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    17701 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    17761 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    17821 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    17881 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    17941 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    18001 ccgtgtttgg tcatatcgcc catggtttca ccagtaccct ggtcgattac acgcgtacct
    18061 accggcactt taattgtgac gtctttaccg cgcttaccgg tacagtcgcg gcttgcgcca
    18121 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    18181 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    18241 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    18301 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    18361 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    18421 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    18481 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    18541 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    18601 gcttccgcca gggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    18661 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcaatt
    18721 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    18781 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    18841 caggtgtaat cggtgagctt tgtaaatatc tcaaccagac tggtgttaaa aaacatcacc
    18901 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    18961 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    19021 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttaccaaa cagcccggcg
    19081 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    19141 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    19201 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    19261 cagcaaattg ctgtggtgag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    19321 tattccctgc aaagcgcatt tgcgaattaa cg
//
LOCUS       DAOTRK010000090        19127 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.090, whole genome shotgun sequence.
ACCESSION   DAOTRK010000090 DAOTRK010000000
VERSION     DAOTRK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.090"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(10..97)
                     /locus_tag="QIE41_003013"
     tRNA            complement(10..97)
                     /locus_tag="QIE41_003013"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Ser,seq:gga)
     gene            complement(351..569)
                     /gene="infA"
                     /locus_tag="QIE41_003014"
     CDS             complement(351..569)
                     /gene="infA"
                     /locus_tag="QIE41_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDS3531879.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(854..1558)
                     /gene="aat"
                     /locus_tag="QIE41_003015"
     CDS             complement(854..1558)
                     /gene="aat"
                     /locus_tag="QIE41_003015"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDS3531880.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1600..3321)
                     /gene="cydC"
                     /locus_tag="QIE41_003016"
     CDS             complement(1600..3321)
                     /gene="cydC"
                     /locus_tag="QIE41_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDS3531881.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3322..5088)
                     /gene="cydD"
                     /locus_tag="QIE41_003017"
     CDS             complement(3322..5088)
                     /gene="cydD"
                     /locus_tag="QIE41_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDS3531882.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEELFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFSTLLAHRQEEI"
     gene            complement(5211..6176)
                     /gene="trxB"
                     /locus_tag="QIE41_003018"
     CDS             complement(5211..6176)
                     /gene="trxB"
                     /locus_tag="QIE41_003018"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDS3531883.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6721..7215
                     /gene="lrp"
                     /locus_tag="QIE41_003019"
     CDS             6721..7215
                     /gene="lrp"
                     /locus_tag="QIE41_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDS3531884.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7350..11417
                     /gene="ftsK"
                     /locus_tag="QIE41_003020"
     CDS             7350..11417
                     /gene="ftsK"
                     /locus_tag="QIE41_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDS3531885.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAQEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQSAQQSYYAPA
                     PEQSVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPVAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKTPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11576..12187
                     /gene="lolA"
                     /locus_tag="QIE41_003021"
     CDS             11576..12187
                     /gene="lolA"
                     /locus_tag="QIE41_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDS3531886.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGTVDAAKFTFTPPQGVTVDDQRK"
     gene            12198..13541
                     /gene="rarA"
                     /locus_tag="QIE41_003022"
                     /gene_synonym="mgsA"
     CDS             12198..13541
                     /gene="rarA"
                     /locus_tag="QIE41_003022"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDS3531887.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13632..14924
                     /gene="serS"
                     /locus_tag="QIE41_003023"
     CDS             13632..14924
                     /gene="serS"
                     /locus_tag="QIE41_003023"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDS3531888.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15163..17607
                     /gene="dmsA"
                     /locus_tag="QIE41_003024"
     CDS             15163..17607
                     /gene="dmsA"
                     /locus_tag="QIE41_003024"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDS3531889.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17618..18235
                     /gene="dmsB"
                     /locus_tag="QIE41_003025"
     CDS             17618..18235
                     /gene="dmsB"
                     /locus_tag="QIE41_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDS3531890.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18237..19100
                     /gene="dmsC"
                     /locus_tag="QIE41_003026"
     CDS             18237..19100
                     /gene="dmsC"
                     /locus_tag="QIE41_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDS3531891.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
BASE COUNT         4774 a         4841 c         5106 g         4406 t
ORIGIN      
        1 ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt atacacactt
       61 tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct accaaacctc
      121 atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag aagaatttta
      181 atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta catcgagaca
      241 agttatggac ttatacttcc aaagtacttc atacatatca caaaataaaa aggccggtta
      301 aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa tcagcgacta
      361 cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt cactttgtcg
      421 cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc agtaaccacg
      481 tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag aacggtacct
      541 tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact accgtaattt
      601 gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg ctaaatcgca
      661 acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca aaagcgcaac
      721 gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat tccttatcgg
      781 cagcggggta atgagcgtaa ccaactctgc gaccgcaatt ataacactct ggggagaaat
      841 gtgccgaaaa cattcattct tgtggtgaaa acaagcaccg tggtacccag aaattattcg
      901 gcaatcgtcc gaggcgcatt tgattgagat aattgaggta atcccggcgg ggaatttcgc
      961 aggcaccaag cgacgctgtg tgatcgttaa gtacctggca gtcgataagc ttaccgccat
     1021 gaccaataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt tccatccggc
     1081 tgaacatgga ctcaccacaa aatagcgttc cctgggccac gccgtacata ccgccgacaa
     1141 gctcatcttc acgccagact tcaatggagt gggcatgacc gagttcgtga aggcgatggt
     1201 aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg gcacagcctt
     1261 caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt ttatgaaatc
     1321 gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcgg ggatcgggcg
     1381 accaccagag gatggggtcg ccgggagaaa accacggaaa aataccacgc tggtaagcca
     1441 ttaacaggcg tgcagggcta agatcgcccc caagtgccag caggccgtta ggctcacgta
     1501 atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc aggcgcatga
     1561 ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct gcttaaactg
     1621 gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa taatttgccc
     1681 gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat gggtgaccat
     1741 taacaccgtt ttctcatgca tcatttctgc aagcaattca aggatctgac tttcggttgt
     1801 ggcatctaag ccttctgtag gttcatccag caataccagc ggcgcatcat gtaacagcgc
     1861 acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc cttcacctaa
     1921 ccaactgttg agacctgcat cctcgagcag cttctccaga ccaacgcgac gtaagatctc
     1981 cgccagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac gcagcgtggc
     2041 gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag tcgcttcatt
     2101 caggctggct atggggctac cgttaagcaa aatctcgccc tgttgcgggt cccatgcgcg
     2161 ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa tcgctatatg
     2221 ttctccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt gctccggata
     2281 agtgaactga acatcccgta acgtcaacga aacgcgatcg gcaacacgag tttgggtatc
     2341 agggaaggtg acttccggtt tttgatccgt taagtcagtg atccgaacgg cagaggcaat
     2401 gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa acgcggctaa
     2461 cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc caacgccgcc
     2521 agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca ttatcgcttg
     2581 cgacaatgcg gtgagttcag attgacgtcg ttgcgcttcc agccactgaa tttcggtatt
     2641 ctctaactgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag cttgcccttg
     2701 cagccaggcc gtcagttgtt ggcgatactg cccgcgaaga tgagtcagat tttgcccggt
     2761 gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta acataatgcc
     2821 gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgattgtca ccaccataat
     2881 caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat cgagcgtatc
     2941 aacatccgcc accacgcggt tgagcaattc gccctgacga tagcgcgcca gtccggcagg
     3001 ggagaggggc agcaatttgc tgaaggtata aatgcgcaga tgctgcaaca cgcggaaagt
     3061 cgcgtcgtga cttaccagac gttcaaaata gcgcccggca gtacgggtga ttgctgcacc
     3121 acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa cccccgctac
     3181 cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg cgagcagcgt
     3241 gacaattgcc agcacaatac caagacttaa catccattta tgacgtttat acagtgccag
     3301 atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagca atgtggagaa
     3361 aggaccacca gccacgctta attccgcgta acgtccttgc tcaataatct gaccatcctg
     3421 cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga ccattaacgt
     3481 tgtctggcgc agagaggcgg cattaagcgc ctccattacg cgctgttcac tgtgagcatc
     3541 aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta atgctcgggc
     3601 caccgccacg cgctgtgcct gccccacgga aaggcgggca gcctggtcgc caacaggcgt
     3661 atcaacgcct tgtggcagga gcggtagaaa ctcactgacc caggcgttat ccagcgctgt
     3721 ttgtaactcg tgttcgctgg catcaggtcg tgccagtagt acgttatccc gtagtgttgc
     3781 tgccggtaat tgtgggtttt gcccaaccca ggagagatgt ttacgccatg attccggtga
     3841 taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga gaaaaccaga
     3901 aagcgcgttc agcagtgagc ttttacctga accgctgcga ccaaccaaca cggcacgttg
     3961 gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttctg gtgatgtaat
     4021 aaacagctcc tcggcttcaa tggtcaccgg atcggtcgat gctaattccg cctcaccacg
     4081 ctgcggatgg gcaagcgggg tttccataaa cgttttcaaa ctgtcagctg caccaacagc
     4141 ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa actccggcgc
     4201 aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcatagt gaccaaaatc
     4261 cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg acagcgaggt
     4321 aaaaaattcg agaatgccgg aggataaaaa cgccagccgt agcacttcca ttgtccgttg
     4381 gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac gaccaaaaat
     4441 acgcaatgtt tccatgccgc gcaggcgatc gagaaaatgc ccacttaagc gagcaagagc
     4501 gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca taaacaacgg
     4561 aattaacgga gcagtgccca gcagaatgag cgccgcagcc cagttagagg ggaagatagc
     4621 caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt agcgtgcata
     4681 gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc ccgccggttt
     4741 accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac ggcggatggc
     4801 aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga ccacccatgc
     4861 gcgaagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt cacggggaat
     4921 attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg caatgatcaa
     4981 tatgccgctc acaaagccca gcagacgaga aatattcagc caacgttggg agatgacgct
     5041 ttgctgtttt aaccagcggg ttaactcttt ttgacgggat ttattcattg cacgcttagc
     5101 aggtgagtta tcagaattat ttgcagagca atgttacaac gaggaaaaaa taaaggcgac
     5161 ccatagtcgc atggtgtcgc cttctttact tttgttactg atttgtaaaa ttattttgcg
     5221 tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc tgtaccagca
     5281 gatgtaatag cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa gacgccaggg
     5341 atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta gccgttttcc
     5401 agttccagct gcccttcgaa aatggcagta ttcgggctgt gaccgatagc aacaaacaga
     5461 ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag acgaacgcca
     5521 gtgacgccca tctgatcgcc ggtcacctct tccagcgtac ggttggtgtg tagaatgatg
     5581 ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc gcggaaaccg
     5641 tcacggcggt gaatcagatg aacttccgaa gcgatgttag acagatacag cgcctcttca
     5701 accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa gaaaccgtcg
     5761 caggttgcac aagcagaaac cccacggcct ttaaacgctt cctcagaagg aaggccgaga
     5821 tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc gccgttatcg
     5881 ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc aaaaatgatc
     5941 tcagtttcaa atttggtggc atgttcgtgc atgcgctcca ttaataacgg accggtcaga
     6001 tcgtttggat cgccaggcca gttttccact tccgtggtgg tggtcagttg gccgcctttt
     6061 tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcatagac agcagcggtg
     6121 tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt gcccatgaga
     6181 tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga cgtaaaaaaa
     6241 gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc aggtcaaatt
     6301 ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga ttaaatgatg
     6361 aataaacgcc cctagtaatg aatatctggc atgttgtact aaaaatcgat gttttgcttt
     6421 gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt attcttatat
     6481 gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta cgtatggcgt
     6541 ggacagacgc cattcgtgat gtcgatagca gccacaaggc aacggtcttc tcaccgtaga
     6601 cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg gtagcgactc
     6661 tgaacagtga tgtttcaggg tcagacagga gtagggaagg aatacagaga gacaataata
     6721 atggtagata gcaagaagcg ccctggcaaa gatctcgacc gtatcgatcg taacattctt
     6781 aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg tgtgggactt
     6841 tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt tattcagggc
     6901 tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt cgttgagatt
     6961 actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt acaaaaactt
     7021 gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt gaaaacacgc
     7081 gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg tctgcctggc
     7141 gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa tcgtctggtt
     7201 attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta cactcctgtt
     7261 aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta gcatcgggca
     7321 ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa gacaaagaag
     7381 tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg atccttattg
     7441 tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg gaccccagct
     7501 ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc ggtgcatggc
     7561 tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc gtcattattg
     7621 tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt gattattttg
     7681 ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt ggtctggcgg
     7741 caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc agcttactaa
     7801 gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg ctctgcgttt
     7861 gggctgcggg tctgacgctg ttcaccggtt ggtcatgggt gaccattgct gaaaaactcg
     7921 gcggctggat tttaaacatt ctcacctttg ccagtaatcg tacccgtcgc gatgatacct
     7981 gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat cacggcaaac
     8041 agcatgaatc acgccgtgcc cgtattcttc gcggcgcgtt agcgcgtcgt aaacggttgg
     8101 cggaaaaatt cattaatccg atggggcggc aaacagatgc tgcgttgttc tccggtaagc
     8161 ggatggatga tgaagaagag attacctaca ctgcacgcgg tgtggctgcc gacccggacg
     8221 acgtcctctt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac gatccattat
     8281 taaacggtgc gccaattacc gaacctgttg ctgtagcagc tgctgctacc acggcgacac
     8341 aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc tctgttgatg
     8401 ttccacctgc acaacctaca gtagcctggc agcctgtacc ggggccacaa acgggagagc
     8461 cggttattgc tcctgcacag gaaggttacc cacagcagcc acaatatgcg cagcctgcag
     8521 tgcaatataa tgagccgctg caacaacctg tacagccaca gcagccgtat tatgcgccag
     8581 cagcggaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa cctgttcagc
     8641 agccgtatta cgcaactgcg ccggaacagt ctgcgcaaca gtcgtattac gcccctgcgc
     8701 cagaacaatc ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc
     8761 cacagtctac ataccagact gagcaaactt atcagcagcc agtcgctcag gagccgttgt
     8821 accaacagcc gcaacctgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag
     8881 agacaaaacc cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgtgccc
     8941 gtgaacgtga acaacttgcg gcctggtatc aaccgattcc tgaaccggtt aaagaaccag
     9001 aaccgatcaa atcttcgctg aaaacacctt ctgttgcagc agtacctcca gtggaagccg
     9061 ctgccgctgt ttcccctctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg
     9121 ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag cgctggaccg cgtccacagg
     9181 tcaaagaggg gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc
     9241 gtgaactggc gtcttacggt attaagctgc cctcacagcg tgcggcagaa gaaaaagccc
     9301 gtgaagctca gcgcaatcag tacgattccg gcgatcagta taacgatgat gaaatcgatg
     9361 cgatgcagca ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg
     9421 aacagtatca acatgatgtg cccgtgaacg cagaagatgc agatgctgcg gcagaggctg
     9481 aactggctcg tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg
     9541 gggcgaatcc gttcacgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg
     9601 atggtccaca cgaaccgttg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac
     9661 cgattgcacc gcagcaacag tatcagcagc cgcagcaacc agttgcgccg cagccgcagt
     9721 atcagcagcc gcaacagccg gttgcaccgc agcagcaata tcagcagcca caacaaccgg
     9781 ttgcgccgca gcagcaatat cagcagccgc aacagccggt tgcacagcag ccgcaatatc
     9841 agcagccgca acagccagtt gcgccgcagc cgcatgatac cctgcttcat ccgctgttga
     9901 tgcgcaatgg tgacagccgt ccgttgcata aaccgacgac gccgctgcct tctctggatt
     9961 tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt gaacaaatgg
    10021 cgcgtctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtc gtcaattact
    10081 ctccggggcc ggttatcact cgctttgaac ttaacctggc accgggcgta aaagcggcgc
    10141 gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg gtgcgtgtcg
    10201 ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa aaacgacaaa
    10261 ccgtttatct gcgcgaagta ttggataacg ccaaattccg cgataatccg tcgccattaa
    10321 ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg gcgaaaatgc
    10381 cgcacttgtt ggttgcgggg actaccggtt ccggtaagtc tgtcggggtg aacgcgatga
    10441 tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg atcgacccga
    10501 aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa gtcgttaccg
    10561 acatgaaaga tgctgccaac gctttgcgct ggtgcgtaaa tgaaatggaa cgtcgttata
    10621 agctgatgtc cgccctaggt gtgcgtaatc tggcgggtta taacgaaaaa attgctgaag
    10681 ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac agtatggatg
    10741 cccagcatcc ggtgctgaaa aaagaaccgt acattgtggt gttggtggac gaatttgccg
    10801 acctgatgat gacggtaggt aaaaaagtgg aagagcttat agcacgtctg gcgcaaaaag
    10861 cccgtgccgc gggtatccac ctcgtactgg caacgcagcg tccatcggtt gatgttatta
    10921 ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc agtaagattg
    10981 actcacgtac cattcttgat caggctggcg cggaatcact gctgggtatg ggggatatgc
    11041 tttactctgg gccgaactcc acgttgccgg tacgtgttca tggtgctttt gttcgcgatc
    11101 aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag tatgttgatg
    11161 gcatcacctc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc gctgaagagc
    11221 tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa gcatcaattt
    11281 ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt atcgaacaga
    11341 tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa gtgctggccc
    11401 caccgccgtt tgactaatta atgcatcgta ggccggataa ggctcggtag cgtcgcatcc
    11461 ggcactctaa caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg
    11521 gaatagagag tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa
    11581 aaaaattgcc atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga
    11641 tgccgcaagc gatcttaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac
    11701 acaaaaagtg actgacggta gcggcgcggc ggtgcaggaa ggccagggcg atctgtgggt
    11761 gaaacgtcca aacttattca actggcatat gactcaacct gatgaaagca ttctggtttc
    11821 tgacggtaaa acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct
    11881 gaaagatgcc accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg
    11941 gcagcagtac aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa
    12001 tggcaatctg aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag
    12061 cgcggtggag caggacgatc agcgcagcag ttatcagctg aaatcccagc aaaatgggac
    12121 tgtggatgca gcgaaattta ccttcacccc gccgcaaggc gtaacggtag atgatcaacg
    12181 taagtagagg cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac
    12241 ctctggccgc gcgtatgcgg ccagaaaatt tagcacagta tatcggccag caacatttgt
    12301 tggctgcggg gaagccgttg ccgcgtgcta tcgaagccgg gcatttgcat tctatgatcc
    12361 tctgggggcc accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga
    12421 acgctgatgt ggaacgtatt tctgccgtta cttctggcgt gaaagagatt cgcgaggcga
    12481 tcgagcgcgc tcggcaaaac cgcaatgctg gtcgccgcac tattcttttt gttgacgaag
    12541 ttcaccgatt caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca
    12601 tcacttttat tggcgcaacc actgaaaacc cgtcgtttga acttaactcg gcactgcttt
    12661 cccgtgcccg tgtctatctg ctgaaatccc tgagtacaga ggatattgag caggtgctta
    12721 ctcaggcgat ggaagacaaa acccgtggct atggtggtca ggatatagtt ctgccagatg
    12781 aaacgcgacg cgccattgct gaactggtga atggcgacgc gcgccgggcg ttaaatacgc
    12841 tggaaatgat ggcggatatg gccgaagtcg atgatagcgg taagcgggtc ctgaagcctg
    12901 aattactgac cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct
    12961 tttacgatct catttccgca ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc
    13021 tgtactggta tgcgcgaatt attactgccg gtggcgatcc gttatatgtc gcgcgtcgct
    13081 gtctggcgat agcgtcggaa gatgtgggta atgctgatcc acgagcgatg caggtggcaa
    13141 ttgcggcctg ggattgcttt acccgagttg gcccggcgga aggagaacgc gccattgctc
    13201 aggcgattgt ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag
    13261 ccgcgctggc ggatgctcgc gaacgtccgg attatgacgt gccggttcat ttgcgtaatg
    13321 cgccgacgaa attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg
    13381 aagcaaacgc ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct
    13441 attatttccc gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg
    13501 ctgaacagga tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg
    13561 ttgttactgt atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc
    13621 acaggataag catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa
    13681 aactggcacg ccggggcttt aagctggatg tagataagct gggcgctctt gaagagcgtc
    13741 gtaaagtatt gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat
    13801 ccattggcca ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca
    13861 aactgggcga agagctggat gcagcaaaag ccgagctgga tgctttacag gctgaaattc
    13921 gcgatatcgc gctaaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg
    13981 aaaatgacaa cgttgaagtc agccgctggg gcaccccgcg tgagtttgac tttgaagttc
    14041 gtgaccatgt gacgctgggt gaaatgcact ctggcctcga ctttgcagcc gcagttaagt
    14101 tgaccggttc ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactat
    14161 cgcagtttat gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt
    14221 acctggttaa ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc
    14281 tgttccatac tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa
    14341 cggcagaagt tccgctgacc aacctggtgc gcggtgaaat catcgatgaa gatgatctgc
    14401 caattaagat gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg
    14461 acacccgtgg tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc
    14521 gcccggaaga ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc
    14581 agttgctggg cctgccgtac cgtaaaatca tcctttgtac cggcgacatg ggctttggtg
    14641 cttgcaaaac ttacgacctg gaagtatgga tcccggcaca gaacacctac cgtgagatct
    14701 cttcctgctc caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgccgcagca
    14761 agtcggacaa gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc
    14821 gtacgctggt tgcagtaatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag
    14881 aagttctgcg tccgtatatg aacggactgg aatatattgg ctaataccca atttttctga
    14941 atctaaaaag cgcctgcggg cgcttttttt gtctcccttt gataccgaac aataattact
    15001 cctcacttac acgtaatact actttcgagt gaaaatctac ctatctcttt gattttcaaa
    15061 ttattcgatg tatacaagcc tatatagcga cctgctatag aaataattac acaatacggt
    15121 ttgttactgg aatcaatcgt gagcaagctt gagtgagcca ttatgaaaac gaaaatccct
    15181 gatgcggtat tggctgctga ggtgagtcgc cgtggtttgg taaaaacgac agcgatcggc
    15241 ggcctggcaa tggccagcag cgcgttaaca ttacctttta gtcggattgc gcacgctgtc
    15301 gatagcgcca ttccaacaaa atcagacgaa aaggttatct ggagcgcctg tacagttaac
    15361 tgtggtagtc gctgcccgct acgtatgcac gtcgtggacg gtgaaatcaa atatgttgaa
    15421 acggataata ctggcgatga caactatgac ggcctgcacc aggtgcgcgc ctgtctgcgt
    15481 ggtcgttcca tgcgtcgccg tgtctacaat ccggaccgcc tgaaatatcc gatgaaacga
    15541 gtcggagcgc gcggtgaagg caaattcgag cgcattagct gggaagaagc ctacgacatc
    15601 atcgcgacca atatgcagcg cctgatcaaa gagtacggca acgaatccat ctatctgaac
    15661 tatggcaccg gtacgctggg tggcaccatg acccgctcct ggccgccggg aaataccctg
    15721 gtcgctcgcc tgatgaactg ctgcggcggt tatctgaacc attacggtga ctactcttcc
    15781 gcgcaaattg cggaaggttt gaactatacc tacggcggct gggcagatgg taacagcccg
    15841 tcggatatcg aaaacagtaa actggtagtg ctgtttggta ataaccctgg cgaaacgcga
    15901 atgagtggcg gtggggtgac ttactatctt gaacaggcgc gtcagaaatc taatgctcgc
    15961 atgatcatca tcgatccgcg ctataccgac accggagccg ggcgcgaaga tgagtggatc
    16021 cctattcgtc cgggaacaga tgccgcactg gttaacggtc tggcgtatgt catgatcacc
    16081 gaaaacctgg tggatcaggc attcctcgat aaatattgcg ttggctacga cgagaaaaca
    16141 ctgcctgcca gtgcgccgaa aaatggtcac tataaagctt atattctggg tgaagggcca
    16201 gatggcgtag ctaaaacgcc ggaatgggcc tcgcaaatca ccggtgttcc ggcagacaaa
    16261 atcatcaaat tggctcgtga aatcggtagt accaaaccgg cgtttatcag ccagggatgg
    16321 ggcccgcagc gtcacgctaa cggtgaaatc gcaacccgtg ctatctcgat gctggcgatt
    16381 ctgaccggta acgttggtat taatggaggg aacagcggcg cgcgtgaagg ttcatacagc
    16441 ttaccgtttg tgcgtatgcc gaccctcgaa aacccgatcc agaccagcat ttctatgttt
    16501 atgtggaccg atgccattga acgtggcccg gaaatgacag cgctgcgtga tggtgtgcgc
    16561 gggaaagata agctggatgt gccgatcaaa atgatctgga actatgccgg taactgcctg
    16621 attaaccagc attctgaaat caaccgtacc catgaaatcc ttcaggatga taagaagtgc
    16681 gagctgattg tggttatcga ctgccacatg acctcatcag cgaaatatgc tgacatcctg
    16741 ctgcctgact gcaccgcttc cgaacagatg gactttgcac tggatgcatc ctgcgggaat
    16801 atgtcttacg tgattttcaa cgatcaggtg attaaaccgc gctttgaatg taagaccatc
    16861 tatgaaatga ccagcgaact ggcaaaacgt cttggcgttg agcaacagtt tactgaaggc
    16921 cgtacacagg aagagtggat gcggcatctg tatgcccagt cgcgggaagc gattcctgaa
    16981 ctgccaacgt ttgaagagtt ccgcaagcag gggatcttta aaaagcgcga cccacaaggg
    17041 caccacgttg cttataaagc tttccgtgaa gatcctcagg caaatccact gaccacgcca
    17101 tcgggtaaaa ttgagattta ttcgcaggcg ctggctgaca ttgccgcgac ctgggaattg
    17161 ccagaaggcg atgtgatcga tccacttccg atctatacgc cgggctttga aagttatcag
    17221 gatccgctga acaaacaata tccgctgcag cttactggct tccactacaa atctcgcgtt
    17281 cactcaactt acggcaacgt tgatgtgctg aaagcggctt gccgtcagga aatgtggatc
    17341 aacccgcttg atgcccaaaa acgcggtatc aacaacggcg ataaagtcag gatctttaac
    17401 gatcgtggtg aggttcatat tgaggcgaaa gtgacgccac gaatgatgcc gggtgtggtc
    17461 gcactgggtg aaggtgcctg gtatgacccg gatgcaaaac gtgtcgataa gggaggttgt
    17521 atcaacgtac tgaccactca acgtccgtct cctctcgcta aggggaatcc gtcacataca
    17581 aaccttgttc aggttgaaaa ggtgtaagga gtaaccgatg acaacccagt atggattttt
    17641 tattgattcc agccgttgca ccggttgcaa aacctgcgag ctggcctgta aagactataa
    17701 agatctgacg ccggaagtca gcttccgccg catttatgaa tatgctggtg gcgactggca
    17761 ggaagataac ggtgtctggc accagaacgt gtttgcctac tatctgtcga tttcgtgcaa
    17821 ccactgcgaa gatccggctt gtactaaagt ctgcccgagt ggtgcgatgc ataaacgtga
    17881 agatggtttt gttgtagtcg atgaagatgt gtgcattggc tgccgctact gccatatggc
    17941 ttgcccgtat ggcgcaccgc aatataacga aacgaaaggc catatgacca aatgcgatgg
    18001 ttgttatgac cgtgttgcgg aggggaaaaa gccgatctgt gttgaatctt gcccgctgcg
    18061 ggcgctggat ttcggcccta tcgacgagct gcgtaaaaaa catggcgatc tggcggccgt
    18121 tgcgccgttg ccgcgagctc actttaccaa accgaatatt gtgatcaaac ccaatgccaa
    18181 cagccgcccg accggggata ccactggcta tctggcaaac ccgaaggagg tgtgagatgg
    18241 gaagtggatg gcatgaatgg ccgctgatga tcttcacggt cttcgggcaa tgtgtagcgg
    18301 gtggttttat cgttctggct ttggcgctgc tcaaaggcga cctgcgagca gaagcccagc
    18361 agcgtgttat tgcctgcatg tttggtttat gggtgctgat gggcattggc tttatcgcct
    18421 ctatgcttca tcttggttca ccgatgcgcg cctttaactc gctcaaccgg gtaggggctt
    18481 cagcactcag taacgaaatc gccagcggtt cgatcttttt tgctgttggt ggcatcggct
    18541 ggttgctggc aatgctgaaa aagctgtcac cggcattgcg tacgctctgg ctgatagtga
    18601 cgatggttct tggcgtcatc tttgtctgga tgatggtgcg tgtgtataac agcattgata
    18661 ccgttccgac ctggtacagc atctggacgc cgatgggctt cttcctgacg atgtttatgg
    18721 gcggcccgtt actgggttac ctgttgctga gtctggctgg cgtcgatggc tgggcgatgc
    18781 gtctgctgcc agccatttct gtactggccc tggtagtgag tggcgtggtg tcagtgatgc
    18841 agggcgcaga gctggcgact attcatagct ctgtgcagca ggccgcagcg ctggtgccgg
    18901 actatggtgc gctgatgtcc tggcggatcg tgcttttggc tgttgccctg tgcttgtgga
    18961 ttgcaccaca gctaaaaggt tatcagcctg cggttccgct actttccgta tcattcattc
    19021 tgcttctggc aggggaatta atcggtcgtg gcgtattcta tggtttgcat atgaccgtgg
    19081 ggatggccgt cgcaagctaa tcataacaac cggggtttcg gccccgg
//
LOCUS       DAOTRK010000091        18816 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.091, whole genome shotgun sequence.
ACCESSION   DAOTRK010000091 DAOTRK010000000
VERSION     DAOTRK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.091"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(33..992)
                     /gene="ybtA"
                     /locus_tag="QIE41_003027"
     CDS             complement(33..992)
                     /gene="ybtA"
                     /locus_tag="QIE41_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HDS3531892.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            1249..2961
                     /gene="ybtP"
                     /locus_tag="QIE41_003028"
     CDS             1249..2961
                     /gene="ybtP"
                     /locus_tag="QIE41_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HDS3531893.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSEN
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            2948..4750
                     /gene="ybtQ"
                     /locus_tag="QIE41_003029"
     CDS             2948..4750
                     /gene="ybtQ"
                     /locus_tag="QIE41_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HDS3531894.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGSASGEWVHE"
     gene            4743..6023
                     /gene="ybtX"
                     /locus_tag="QIE41_003030"
     CDS             4743..6023
                     /gene="ybtX"
                     /locus_tag="QIE41_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HDS3531895.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            6051..7355
                     /gene="ybtS"
                     /locus_tag="QIE41_003031"
     CDS             6051..7355
                     /gene="ybtS"
                     /locus_tag="QIE41_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HDS3531896.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(7549..8811)
                     /locus_tag="QIE41_003032"
     CDS             complement(7549..8811)
                     /locus_tag="QIE41_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDS3531897.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLQKGIEEKGLLEV
                     ASRTRQHLSNIIRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVHYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQEHNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(8973..9048)
                     /locus_tag="QIE41_003033"
     tRNA            complement(8973..9048)
                     /locus_tag="QIE41_003033"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9013..9015),aa:Asn,seq:gtt)
     gene            complement(9149..9946)
                     /gene="mtfA"
                     /locus_tag="QIE41_003034"
     CDS             complement(9149..9946)
                     /gene="mtfA"
                     /locus_tag="QIE41_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDS3531898.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            10040..10129
                     /locus_tag="QIE41_003035"
     tRNA            10040..10129
                     /locus_tag="QIE41_003035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10074..10076,aa:Ser,seq:cga)
     gene            complement(10799..11449)
                     /gene="zinT"
                     /locus_tag="QIE41_003036"
     CDS             complement(10799..11449)
                     /gene="zinT"
                     /locus_tag="QIE41_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDS3531899.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRHNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDTQQSLLNEMENWPTYYPYQLSSKEVVEEMMSH"
     gene            complement(11706..12341)
                     /gene="msrQ"
                     /locus_tag="QIE41_003037"
     CDS             complement(11706..12341)
                     /gene="msrQ"
                     /locus_tag="QIE41_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDS3531900.1"
                     /translation="MRLTAKQVTWMKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            complement(12342..13346)
                     /gene="msrP"
                     /locus_tag="QIE41_003038"
     CDS             complement(12342..13346)
                     /gene="msrP"
                     /locus_tag="QIE41_003038"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDS3531901.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(13455..13868)
                     /gene="hiuH"
                     /locus_tag="QIE41_003039"
     CDS             complement(13455..13868)
                     /gene="hiuH"
                     /locus_tag="QIE41_003039"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDS3531902.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            14001..14672
                     /gene="hprR"
                     /locus_tag="QIE41_003040"
     CDS             14001..14672
                     /gene="hprR"
                     /locus_tag="QIE41_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDS3531903.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            14672..16030
                     /gene="hprS"
                     /locus_tag="QIE41_003041"
     CDS             14672..16030
                     /gene="hprS"
                     /locus_tag="QIE41_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDS3531904.1"
                     /translation="MKRLSITVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRILITSFLDTNGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKLNVFRITLPQ
                     RN"
     gene            complement(16137..16988)
                     /gene="hchA"
                     /locus_tag="QIE41_003042"
     CDS             complement(16137..16988)
                     /gene="hchA"
                     /locus_tag="QIE41_003042"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDS3531905.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPNKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYSS"
     gene            complement(17580..18695)
                     /locus_tag="QIE41_003043"
     CDS             complement(17580..18695)
                     /locus_tag="QIE41_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDS3531906.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADREGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
BASE COUNT         4558 a         4409 c         4933 g         4916 t
ORIGIN      
        1 tcagggagga gtttaggggg gcgcgacccc ggttacatcc cgcgtttaaa ggtcgaagga
       61 gttacgccaa actgtttctg gaaggcggca gagaaatggc tggcgttggc atagcccaga
      121 tcggccgcca ccgtcatgac ggaggtattg ccgttggcca ggcgtcttct ggcttcctgc
      181 attcgttcag cctgaaacag gccatagacg ctgttgttga acagaacgcg gaagcctcgc
      241 ttaattttga gaacgctcag accggactct ctggccagct ccgcgacgcc cggcgcttgc
      301 gtcagatcag ccagcagcag atccttcgcc cgcagcagtt tctggcgttc catcgggttg
      361 aagtggcagg agaggcaact ggcgtcttcg cgatgttcat ccagcaccag gctgagcatg
      421 accagacttt gcccctgcaa ccatagcgtc gacggctttt gttcatgact gaagccgccg
      481 tgcatgcgca tcagcgcgtg acgcaaggcc tgagccgtta agtgggtttc agcattacaa
      541 cgctgctgct ggcaacagca gtgggagtcg atcttattct taatgaccgc gctaatatcc
      601 ggcacccaca gcgcgagggt ttcagggctg aaggagaggg taatgctctc gaatcggcca
      661 caataggagg cgctgccgcg acagtctggc gtatgggtaa tgtaatggcg attgcgcgca
      721 agctcccagt cggtgtggcg ccggagtccc tgaatcgcaa agcgcgatgt gccttccagg
      781 atgcacgaga agttaatcct gcccagatcg tcgtgtagct gcaacgtctc tggccgcgca
      841 aaatggcccc tccagagcaa aatatccata ccttcctgca acgaacagcg cagcagttca
      901 cactgagcag gaatattgcc atcgtttgca ggtttcccga ccaccaactg gtgaatagag
      961 atttcagatt gcgtttgcgg tgactccgtc atgacctggt tatctccctg tggcttagac
     1021 caatgcgggt ataaatagaa ccgaatgggg tagatgagtt attgataatg gttattattc
     1081 acattaattc tcagaacgcc accctgaatt gtcgtttttg gcgtccgtaa aaattcatca
     1141 ggaaattcag ttactcccat gtcatctcaa tcatccaata ctgaatcgct gtcccgattt
     1201 cctctctggc aggtgattac accggttcgc aggaaggtta ttttagctat ggcgctggcg
     1261 gggctggcgg cattaaccag cctgggggcg ctcctttttc tggcgtggag cctgcgtgac
     1321 attcgcgcga cgcctgacgc tattccggcc tggccgctgg gcggcgtgat cggctgcgtg
     1381 gtcttaactt ttgttctgcg cttacaggcg ttcaacacct ctcattacgc ggcttttcat
     1441 ctggagaaca ttctgcgcag caggttagcc cgtaaagcat tgcagcttcc gcctggcgtg
     1501 ttacagcaaa tgggcagcgg gtcagtggcg aaagtgatgc tggatgacgt gaagtcgtta
     1561 catatttttg tggccgacag cacgccgctc tatgcccgcg cgatcatcat gccgctggcg
     1621 acaatcgtta tcttgttctg gctggactgg cggctggcaa tcgcgacgct gggggtactg
     1681 gcgtttggat cggttgttct cgtgctcgcc cgccagcgtt cggaaaatat ggctcagcgt
     1741 tatcataagg cgcgcgagca ggttagcgcg gcggttattg agttcgtgca ggccatgccc
     1801 gtggtgagaa cgtttgatag cggcagcacc agttttttgc gctatcaacg cgcccttgaa
     1861 gagtgggtcg atgtgctcaa aacctggtat cgcaaagccg gtttttcagc gcgtttttcc
     1921 ttctcgattc tgaatcctct cccgaccctg tttgtcctga tctggtcggg atacggcctg
     1981 ttgcactatg gcagtttcga ttttatcgcg tgggtggccg ttttactgct gggcagcgga
     2041 atggccgaag ccgtaatgcc aatgatgatg ctcaataacc tggtcgcgca aacgcgttta
     2101 agcattcagc gtatttatca ggttctcgcg atgccggagt tatcgctgcc gcagtctgac
     2161 cagcagccgc aagaggcgag cattaccttt gagcaggtga gctttcatta tccgcaagcg
     2221 cgtactggcg ccgcgttgca ggaggtgagc tttcatgtgc ctgccgggca aattgtggcg
     2281 ctggtcgggc caagcggcgc cggaaaaagc accgtggcgc gcttgctgct gcgttacgcc
     2341 gacccggaca aaggccatat ccgtattggc ggcgtggatc tgcgtgatat gcagacggac
     2401 accctgatga agcaactctc gtttgtgttt caggacaact tcctttttgc cgacacgata
     2461 gccaataaca ttcgtctggg cgcgccggat acgccgctgg aggcggttat agcggcggcc
     2521 agagtggcgc aggcccatga ttttattagc gctctgccag aaggttacaa cactcgagtc
     2581 ggggaacgtg gggtatttct ctccggcggc cagcggcagc gcattactat cgcccgggcg
     2641 cttttgcagg atcgccccat cctggtgctc gatgaggcga cggcgtttgc tgacccggaa
     2701 aacgaagcgg cgcttatcaa ggcgctcgcg gcggccatgc gtggccggac ggtcatcatg
     2761 gtcgcgcatc gtctctcaat ggtgactcag gccgatgtga tattgctgtt ttccgacgga
     2821 caactcaggg aaatggggaa ccatacgcaa ctgttggcgc agggcggcct gtatcaacgg
     2881 ctctggcaac actatcagca ggcgcagcat tgggtgccgg gtggaacaca ggaagaggtg
     2941 gtggaaaatg aaagacaata atcctgcgga taacctggcc tggcgcgtca tctggcgcca
     3001 gcttatctcc agcgttggca gtcaggccag gatgctgcga cgcagtatgc tggcgctgtt
     3061 gctggcggca ttcatgcagg ggatcgcctt tgcctgtctt tatccgatca ttgatgcgct
     3121 gttacgggga gacgcgccgc aacttcttaa ctgggctatg gccttcagcg tcgccgcaat
     3181 tgtgacgctg gtgctacgct ggtatggcct gggctttgaa taccgtggtc atctggcgca
     3241 ggctacccat gagttgcgcc tgcgacttgg cgagcagtta cgccgcgtgc cgctggagaa
     3301 gctccagcgc ggcagggcgg gtgaaatgaa cgccttgctg ctgggcagcg tggatgaaaa
     3361 cctcaattat gttattgcga tagccaatat tttgctgctc accattgtca cgccgctgac
     3421 ggcgtcgctg gcgacattgt ggatagactg gcggctgggg ctggtgatgt tgctgatctt
     3481 ccctctgctg gtgccgtttt attactggcg ccgcccggcg atgcggcgac aaatgcagac
     3541 gctgggggaa gcgcaccagc gtctgagcgg cgatatcgtt gaatttgctc aggggatgat
     3601 ggtattgcgc acctgcggca gcgatgccga taaaagccgg gcgctgctgg cgcatttcaa
     3661 tgcgctggaa aacttacaga cccgcactca ccgtcagggc gctggcgcaa cgatgctgat
     3721 cgccagcgtc gtggagttgg gcctacaggt ggtggtgtta tccgggatcg tctgggtagt
     3781 gacgggcact ctgaacctcg cctttttgat tgccgccgtc gcgatgatta tgcgcttcgc
     3841 agaaccgatg gcgatgttta tcagctacac ctcggttgtg gaactgatcg ccagcgccct
     3901 gcaacggatt gagcagttta tggcgatagc accgcttccc gtcgcagagc aaagcgagat
     3961 gccggaacgt tacgatatcc gctttgacaa cgtcagctat cgctacgaag aaggtgacgg
     4021 ccacgcgctt aatcatgttt ctttgacgtt cccggcagcc agtatgagcg cgctggtggg
     4081 tgcctccggc gcaggcaaaa ctacggtcac caaactgtta atgcgctatg ccgatccgca
     4141 gcaagggcag atttctattg gcggcgtcga tattcgccgc ctgacgccgg aacagctcaa
     4201 tagcctgatt tctgttgttt tccaggatgt ctggctgttt gatgacacgc tgctggcgaa
     4261 tatccgtatc gcgcgcccac aggcgacgcg gcaggaggta gaagaggccg cccgcgcggc
     4321 gcagtgcctt gagtttattt cccgtcttcc gcaaggctgg ctgacgccaa tgggagagat
     4381 gggcggccag ctatcgggcg gcgagcgcca gcggatttcc attgccagag cgttattgaa
     4441 aaacgcgccg gtcgtcattc tcgatgaacc gactgccgcg ctggatattg aaagcgagct
     4501 ggcggtgcaa aaagcgatcg ataacctggt tcacaaccgg acggtgatta tcatcgctca
     4561 ccgtttatcc accatcgccg gggccggaaa cattctggtg atggaagagg gacaggtggt
     4621 tgagcagggt actcatgcgc aattgctctc acatcatgga cgttatcagg cgctgtggca
     4681 ggcgcaaatg gccgcgcgcg tgtggcgcga cgacgggggt tccgcgtctg gagagtgggt
     4741 gcatgagtga tgttcagtcg aatgtgaaac ctctgacgtt gacgaccggg cgggtgattt
     4801 ttgctattgc cggcgtctat gtgacgcaga gtctggtatc ggcgctgtct atgcagtcct
     4861 tacccgcgct ggtgcgcgct gctggcggtt cgctggcgct tgccggtgcg acaaccctgt
     4921 ttatgctgcc ctgggcgctg aagtttattt gggcgccgtg gatcgagcgc tggcggcttc
     4981 cgcccggtag ccaggaacgc cgttcccgca tgttaatcct gcgtggtcag gtcgcgctag
     5041 cggcgatcct gactattgcc gcagcgattg gctggtttgg gcgagaaggg ggattccccg
     5101 atacgcaaat cgtcgcgtta tttgttctgt ttatggtggc aggcacggtc gcctccacca
     5161 ttgatatcgc cagcgacggc ttttgtgtcg atcaactgac ccgcacgggt tacggctggg
     5221 gaaacagcgt gcaggtcggc ggcagctatc tgggaatgat gtgcggcggc ggggtgttcc
     5281 tgatgctgtc ggcggcatcc ggctggcctg tcgccatgct gatgatggcg gtgctgatta
     5341 tggcgctgtc actcccgctg tggcgcatta cggagccgac gcgaacagcg actatcccgc
     5401 atgttccggc gttaggttat gcgctaagga ggaagcaggc gcgcctgggc ttactgctgg
     5461 tattgatgct gaattcaggc atgcggtttg tgctgcctct tctggcaccg ctgttgttgg
     5521 atcatgggtt gagcatgtcc gcattgggcg cgctgttcag cggcggcaat attgcagcgg
     5581 gcatagcagg aacgctggcc ggcggattgc tgatgaaata cacctcaccc ggcagagcgc
     5641 tgttgacggc ttatggcgtc caggggatcg cgctgctggc ggtggtgatg acgctcatga
     5701 tggcgccggg tcatctgctg ctgcagattc tccagtgtct ggtcattgtc cagtccattt
     5761 cgctggcctg cgcgctggtc tgtctttacg ccacgctgat gtcgctttca tcgcctttgc
     5821 aggccggtgt cgacttcacc ctctttcaat gtactgacgc ggcaatcgcc attctggctg
     5881 gcgttatcgg tggcgttgtt gctcaacatt ttggctatgc ggcctgcttc ctgtttgccg
     5941 gggcattcac gttgctggcg gcgtgggttg cttatatccg gctgcattcg gcaagagaac
     6001 tgatgacaag cgcaattgat tgatccggga ataacacaga gaggtaaggg atgaaaatca
     6061 gtgaatttct acatctggcg ttaccagagg aacaatggct accgacgatt tctggcgttt
     6121 tacgccagtt cgcagaagaa gagtgttatg tctatgagcg tcaaccctgt tggtatttag
     6181 gcaaagggtg ccaggcacgg ctgcacatta atgccgacgg aacgcaggcg acatttattg
     6241 atgatgctgg ggagcaaaaa tgggcggtgg attccattgc cgactgcgcg cgtcgtttta
     6301 tggcgcatcc tcaggtgaaa ggacgtcggg tatatggaca ggttgggttc aactttgcgg
     6361 cgcatgcgcg ggggattgcc tttaacgccg gggagtggcc gctgctgacg ttaaccgttc
     6421 cccgtgaaga acttattttt gaaaagggaa atgtcaccgt ttatgcggac tccgccgacg
     6481 ggtgccgacg tttgtgcgag tgggtaaaag aggccggtac aacgacgcag aacgcaccac
     6541 tggcggtgga taccgccctc aatggtgagg catataaaca acaggttgcg cgcgccgttg
     6601 cggagatccg ccgtggcgag tatgtcaaag tgattgtctc gcgcgccatt cccctgccat
     6661 cgcggattga tatgcccgcc acgctgttat acgggcggca ggcaaacacg cctgtgcgct
     6721 cgtttatgtt ccgtcaggaa ggacgcgaag cgctgggctt tagcccggaa ctggtgatgt
     6781 cagtgacggg caataaagtg gtcactgaac cgcttgcggg cacccgagat cgcatgggaa
     6841 acccggagca taataaggcg aaagaggcag aactgctgca cgacagtaaa gaggtgcttg
     6901 agcatatcct ttctgtcaaa gaagctattg ctgaactgga ggctgtttgc cagccgggca
     6961 gcgtggtggt tgaagattta atgtcggttc gccagcgcgg cagcgttcag catctggggt
     7021 ctggcgtgag cggtcagctt gcggaaaaca aggatgcctg ggatgcgttt accgtgctgt
     7081 ttccgtcgat taccgcctca ggtatcccta aaaatgctgc tctgaacgcg attatgcaaa
     7141 ttgagaagac gccgcgagag ctttattccg gcgcaatcct gctgctggac gatacgcgct
     7201 ttgatgcggc gctagttctg cgttccgtat ttcaggatag ccagcgctgc tggatacagg
     7261 cgggggcggg aatcatcgcg caatctacgc cggaacgcga actgacagaa acccgggaga
     7321 aattagcgag cattgcgccc tatttaatgg tgtaggagta actgttttct aacctcacaa
     7381 ccagactgtc caaagacaat tgagtcgctc tttgattcct ctgtgactct attgtcttct
     7441 tttgggataa aataggtgtt tttcgtcatg aagttgataa aagtctatat agaaccacct
     7501 cctgtggagg tggtggtaat ttgccagagt gtgggattcg ttagtatact atgggataaa
     7561 cttattttta ccaattgtat aaggcggtgc ataagattct cggcatgctt ccagataatc
     7621 cgaccaccac tgcatcatcg ctttgcgggc ttccaggtgc tctgccttat gaatataagc
     7681 catgcgcacg gtattgtgct cctgatgact catctggcgt tctactgcgt cctttgccca
     7741 taaacccgat tccatcagcg cactgcatgc cattgcccgg aagccgtgac cgcagatatc
     7801 ctttttcgtg tcgtaaccca tcacccgcag tgccttattg accgtgtttt cacacattgg
     7861 cttatacggg ttatggtcgc cggggaagat cagttcatta ttaccggtga tatccttaat
     7921 ctgtttcaga atggcgatgg actgttctga gagggggacg atatgcggca ttcgcatttt
     7981 tgccccgcgg ccggaataat gcacgccaat aatgggttct cgcgtcgcgg gtatcgtcca
     8041 gactcggttt gtgaaatcaa tctctgacca gcgggcgaaa cgcagttcac tggagcgaat
     8101 gaacacatgc agcatcagca gaacggcatg ccgggtcagt tcacggccct gatgatatgc
     8161 cccaatacgt tcaagcagtt caggcagccg ctccagcggc agggcaggat agtgccgtct
     8221 gacaggaggt gtggtcacgc cgccaaggtt tgctgcaggg ttcgtatcga ttaactcctg
     8281 atggaccgca tggcgtatta tgttactcag gtgctgccgt gtgcgggacg caacctccag
     8341 cagacctttt tcctcgatcc ctttctgcag gtcaatgaaa tgacggggtt taagttctga
     8401 tacaggtagg ttcccgatga ccggaaagat gtgattgttc aggctggcaa gcagacggtc
     8461 ggcggtgttc tgcgaccatt tcctgttact tttatgccac gccagcgcca cgtttttaaa
     8521 aactttctcc ggtgttcgtg agccacgtac agcagcccgc tgctgtaccg ggttgatatt
     8581 cagcgccagc attttacgga taccttcacg ttgctgtcgc gcatcagaca gggagatggc
     8641 tggataggca cccagcgcaa tgcgggattc tttaccgcta atacggtatt tgagatacca
     8701 gtggcgggag ccacccggct tgacccgcag atacagaccg tgagaatcgg agactttaaa
     8761 gggtttatca gaaggcttga gggtgcggat ttttgcgtcg gtaagggata tatgggggtc
     8821 actccattat cgaactaacc tgaccccaga tttgaccacc aatttttccc gatgcgtagg
     8881 gtaaaatcaa aatgcaccga gaagagtttt cacactaacc tgttgaatca acatcagata
     8941 aagattcgca aggatgcaag aaaacagaaa attggctcct ctgactggac tcgaaccagt
     9001 gacatacgga ttaacagtcc gccgttctac cgactgaact acagaggaat cgtgagaacg
     9061 gggcgcatat tacttagcgg taccttgtct gtcaacacta aatttcataa gccatttcaa
     9121 ttggttaatt aatctgcaaa gttgttaatt aatgaacatt cgtcgccgaa aacgagtctg
     9181 tatcattagc gtgatgcagt ctctgcaaag gatcttgttg ataaaattgg cagaaacgtt
     9241 gccacaatga agggaaacga ggagcaaaaa gttctggggc gctaaagaaa tattcagaaa
     9301 gtacggcaaa acattcagca ggatcactgg cagcataagc atcgatgctc gccgcattct
     9361 cgccaaccaa ctcgatttct tcctgaatgt tgttcattgc agcatgaaga tcgtgttccc
     9421 agccagcaac ctcacgcaac ggaataaagg gaactccgct ggcgcgatcg ccgttacggg
     9481 tgtccagctt atgagcgact tcatgaataa tcaggttaaa accagaagca tcaaaagaat
     9541 cttgtatatc caaccagttc aaaacgatag gcccttgctg ccagctctga cctgactgaa
     9601 caatacgttg gttatgcacc agaccgatat cgtcttccca ttcatcatcg accacaaatg
     9661 gcgcaggata aattaagact tcatgaaaac catccagcca ttccagtcct aactccagaa
     9721 cgggcaggca aaatagaagt gctatccggc agcttcttaa tgaattcagc tcaaagccct
     9781 gtaaaggaac aagccgtttt tgctgtaaaa aacgttcggc aagagtgact aatttgcttt
     9841 gttcctgttc cgtcagacac gttaaaaggg ggatcgatag tgcttcctgc cagggaaggg
     9901 cagtttgatg tgctgattct tgtactttcc agggccactt aatcatcgtt ttgctcgcaa
     9961 actcgtcact tgaacaaaat tgcacggaca gggactgtta aaatgccaaa tttcctggca
    10021 tcatggcaac catctgaacg gagagatgcc ggagcggctg aacggaccgg tctcgaaaac
    10081 cggagtaggg gcaactctac cgggggttca aatccccctc tctccgccac tttatcaatg
    10141 acttatctcc cgacttcccg ccttgctttt cctaaacaga acaatcgtag aatattcttg
    10201 aagggttaga tcgtcactgt tttctgttcg atactgtgac attcagcact tgattcgcta
    10261 tgggtctgac aggaaggttt cgagcgaaaa tctacagtta ttcagtcgtt ttcttatcgg
    10321 tcaccattat tcttttagac attgatccta tgaaactgcc gcaaagttgc tggcgggaac
    10381 tgaagttgcg tagagaaggg gtcaataatt gagccaaaaa aggaacgcgg ccgtcagatt
    10441 aaagccgcgg gacaaagtcc ataatttcca agagttgttg aatttcccag cttttttgaa
    10501 agcctttgta tcatactaac aagtaaaaaa ggtttaaata ccaaaattaa cctatatttc
    10561 tggttagctt taccatgaca gatgacttgc tcctggagac attggcgcag ccagtctgta
    10621 ttggtgagtt gtgtatttat tgttcgccgt tttctcagtt tttttctaca gacataataa
    10681 aaaagacacg tctcaagcat gagcgtgtct gagtttgtct tatttatttg tcttggtaaa
    10741 ttattgtcgt tactaccagg cactgttctg ttaaataatt gaagtcagcg caaaaaaatc
    10801 aatgagacat catctcctcg accacttctt tgctactcaa ctgatatgga taatacgttg
    10861 gccagttttc catttcattc aataaagact gttgggtatc attacccata aaaatgtgaa
    10921 aatgagatga ttttcgtggt gcaattatat gatcgctaaa ttgaatatat ttaggggctt
    10981 tgctttcagg atctttacat tcgaataagt aacgaacgcc tttcttgcct gatttatagg
    11041 tgagtatttt gtatccatcg taatcatatt tacaggatgt tgtttcatta tgtctatgga
    11101 attcaacaat gccgtcctca atgccaatca tctcgatatc tgtcacataa cctttgcgat
    11161 aataatcttt aatttcagca aatgttttgg ttttatctgc atccgctttc ttctgaaaga
    11221 cggggtcaag tttgccactt tgcagtaaag gataaacgga ttgccagact ccatcccagt
    11281 cactgagcgt tcggttttgt acattggtat catcaaaaac accattagca gctttttgtt
    11341 cgacctctgt taagggtttg ccgtgtgagt gatgaccatg cgaaaaggca ggagcgctaa
    11401 caataaagac gcctaaagca acagccagtt tatgaagacg aatcgccaaa acagtttcct
    11461 ccagagttaa tgtatataat gtgtaatgtt atattgtaac atataacaaa atgcaacaac
    11521 tcttaggaaa cgagtgcaaa gttacctcag gtatattttc ctatcttatt gaaatgacat
    11581 aaattatgtc gatgatttga ggttgatgac aaacgaaaca taacctgatg tgatctgcgt
    11641 aggccggata gggcgctcgc gccgcatccg gcagggataa cgcgtacttt gtcggtaata
    11701 tgaagttata gagataattt gttgtgaacc tgcttgcgta accggttgaa aagggaaagc
    11761 aacttcttat accgtaaggc taaaagcagt accgccagcc cagcgtagat gagtggctgc
    11821 ggtgaaataa tcttcactga ccacagataa tgtattgggg ccaggatcgc gacaagatag
    11881 acgaaattat gcaactgttg ccagtgcttg ccgagttttc gctgcatcgc ctgggttgaa
    11941 gtaaacgcta aagcaagcaa aataacccag ctgataattc ctaacgttaa ataaggccgg
    12001 gtaattaact ccttacctaa taacgccaga ttattcacgc ccagctccag taatgcgtaa
    12061 ctggttaaat gcagtgtcgc ccaggcaaag caccataagc ctaacaggcg gcgagtgcgt
    12121 atcaataacg gctgttttgc gtagcgtgcc agaggtgtga ttaacaaggt cgccagcaaa
    12181 aatttcagtg cagtgcgacc agtaaaatgc tgaatatctt tcactggatc ggcacccagt
    12241 ccaccgtgat tgatcgccca gaccagccag agaaacggca acaatccggc aagatgcagg
    12301 caaactttca tccatgtcac ctgttttgct gtcagacgca tttagaaatt ctcccgcaaa
    12361 tccaggccac gatacagcga tgccacctgc tcggcgtaac cattaaacag tagcgttggc
    12421 tggcgctgta catcgaggat gccgcctgaa ccaataaatc gttcggtagc ctgtgaccag
    12481 cgcgggtgat caacatgcgg attaacgttg gcgtaaaaac cgtattcgtc aggcgctgcc
    12541 agattccagg tggttggcgg acgctcgcgg gtcagcttaa tactgactat cgatttaatc
    12601 cctttaaagc catatttcca cggcacaatc agtcgcaccg gcgcgccatt ttgtggcggt
    12661 aacgccttgc cataaacacc cacggtcatc agtgtgagcg gatgcattgc ttcgtcgaga
    12721 cgcaatcctt cgacataagg atatttcagc ccgccgccga taaagcggtc ctgctggcct
    12781 ggcatctgtt ccggtgcata aattgtttcg aaagcgacat acttcgcatt gctggtgggt
    12841 tcggcaagcg ccagtaattt gtgcagcgga aaaccaatcc acggcaccac catcgaccat
    12901 gcttccacgc agcgcatacg ataaatacgc tcttccagcg ggaagcgacg ggttaaatca
    12961 tcgtgatcga gggtcaatgg ttttgccact tcgccgctga ttttcaatgt ccatggatcg
    13021 gttttcaggc taccagcatt agcggcggga tcggctttat ccagcccgaa ttcatagaag
    13081 ttgttatagc cggaaacttt atctgctggc gtcagtggca ggtcattttg ccaggcggca
    13141 ggcttgctga actccaacgg ttttccggcg ggggcaggtg ggcgatcgtt ccctttaaac
    13201 cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa gtgcagccgc gctgatgccc
    13261 agtgctttta acacctgccg acgcttcata aagaatactg actcggccgt aacatctgat
    13321 tcttttaaaa attgattctt tttcataagc catcacactt ttttgtcgtt agccagcaag
    13381 agccgtttac tgttaatctg tcagggagtg tcctccctaa cagtagcaga gatctgcttt
    13441 tacatattta tgcattaact gccacgatag gttgaatacc catattggct aagtaataaa
    13501 ggcacatgat aatgctcgtt cactttatta atatgaaatt caacggggat ctcagggaag
    13561 aaactttcaa gattttgttt cttgaaatag tccccggttt taaatacgac acggtaatcg
    13621 cccgtagttg cagtttgctc gggccacagt gccttaattc gtccatcctt atctgttttg
    13681 gcggtattaa gttgtaacca gccgttgtcc gctttctttt caagagtgac tgtcacgtcg
    13741 gcagcgggtt ttcctgtttg ctggttcaaa atgtgcacgc taagaatgtt ttgttgtgca
    13801 gcatagacca aagaaggtaa tgaaaatgca gccgttacta cggagagaac taaataacgc
    13861 tttaacatgt ttatatcctt gtcatgtgaa tgagttttga ggatagcgaa aaagcgtcca
    13921 gggtaacctt acagcagcat tacaattttg taatgaacca ggctgtttct ataacatatg
    13981 atttatggca tattattttc atgaagattt tacttattga agataatcaa aggacccagg
    14041 agtgggtaac gcaggggctt tccgaagcgg gttatgtcat tgatgccgtt tctgatggca
    14101 gagatgggct ttatcttgcg ctgaaggatg attatgcatt gatcattctg gatattatgc
    14161 ttccgggtat ggatggctgg cagatcttac aaacgttaag aacagcaaag caaacccctg
    14221 ttatttgcct tactgcaagg gattctgtcg atgacagagt cagagggctg gacagtggag
    14281 caaatgatta tctggtaaaa cctttttcat tttctgagtt gctggcaagg gttcgggcac
    14341 aattaaggca acatcatgct ttgaattcaa cattagaaat cagtggctta agaatggact
    14401 ctgttagtca aagtgtgagc agggacaata tcagtattac actgacgcgc aaggagtttc
    14461 agttactttg gctactggcc tccagagctg gcgaaattat acccagaacg gttattgcga
    14521 gtgaaatttg gggaatcaac tttgatagtg ataccaatac ggtggacgtc gccattcgca
    14581 ggctccgcgc aaaagttgat gatccttttc ctgaaaagct aatcgccaca atccggggga
    14641 tgggctattc attcgtagcg gtaaaaaaat aatgaaaaga ttatctataa ccgtccgttt
    14701 aaccttgctt tttatattgg tgctgtctgt tgctggcgct ggaattgtct ggactctcta
    14761 taatggcctg gcaagtgagt tgaaatggcg cgatgataca acactcatta accggacagc
    14821 gcagatcaag cagttgttaa ttgatggggt aaatccagat acgttacctg tgtactttaa
    14881 ccggatgatg gatgttagtc aggatatctt gatcattcat ggtgatagca tcaacaaaat
    14941 tgttaaccgg acgaatgtca gtgatgacat gttaaataac atacctgcta ctgagacaat
    15001 cagcgcagct ggcatttaca gaagcattat taatgataca gagatagatg ctttacgaat
    15061 taatattgat gaagtttcgc catcattaac ggttactgtg gctaaattgg cttcagccag
    15121 acataacatg cttgaacagt ataaaattaa tagcattata atttgcattg tcgccattat
    15181 actttgctca gtattaagtc cgctgttaat cagaacgggg ttacgagaga tcaaaaagtt
    15241 gagtggtgta acggaagcgc tgaattataa cgataaccgg gagcctgttg aggttagcgc
    15301 attaccgaga gaactaaaac ctcttgggca ggcgttgaat aaaatgcacc aagccttagt
    15361 caaagatttt gaacgcctaa gtcaatttgc tgacgatctc gctcatgaac ttagaacgcc
    15421 aattaatgca ttactgggtc agaatcaggt tacgctcagt caaaccagaa gtatcgctga
    15481 atatcaaaaa acaattgccg gaaacattga agagctggaa aatatttcgc ggttaacaga
    15541 gaacatactg tttcttgcca gggcagataa aaataatgtt ttggtgaaac tggactcgct
    15601 ttctctcaat aaggaagtcg aaaatttgtt ggactatctt gaataccttt cagacgagaa
    15661 agagatttgc tttaaggtcg agtgcaatca gcaaatcttt gcggataaaa ttttactaca
    15721 acgaatgtta tcgaatctta ttgttaacgc tattcgatat tcgccagaaa aatcgcgcat
    15781 tcttataacc agttttcttg ataccaacgg ctctcttaat attgatatcg ccagccctgg
    15841 tacgaaaatt aatgagcctg aaaaactctt ccgtagattt tggcgcggag ataattcgcg
    15901 tcattccgta ggtcagggac ttggtctttc tttagtcaaa gcgattgccg aattgcatgg
    15961 gggaagtgct acgtatcact atctcaataa gcttaatgtg ttccggatta cgttaccgca
    16021 aagaaattaa catagggctt cagtacgcca taaattaacc gtgaaaaacg cagccacgtc
    16081 gtgagtatta acgtggctgc gattgattat gcgcttacat tacaatgtaa cagggattaa
    16141 ctcgagtaag cggccagcat ttcctgcgcc gccagtttac ccaacgcatt cgctgcgaaa
    16201 ggactgtcgc cggtgagaac tttacggtcc ttatgtactc gcccggtgat gtcgtcatta
    16261 ataatgttca tgcccatttt cttcagctct tcgccgaagt accaggtgag atgacccggc
    16321 atatagccta tctctggcgt ttgtttgtct gcggcgtctg ggaatgcgca aatggaataa
    16381 ccattcagtg ggttatcgcc gtggcgaagt gccagaaaag ccgccggacc gtggcaaagg
    16441 gatataacaa aacggtcatt tttgattgcc cactgtaaag cggcagccac gtcctggctt
    16501 tctggtaaac caataagtgc gccatgacca ccaggaacaa agattgctgc atattcgctg
    16561 tcagcgttga ggctggcgac aacatccgcg agtttattcg gattgcggaa caacgattta
    16621 tgctgctcaa agaatggcat tactttttca tctttgtgtg gcatagccca gtattcaaac
    16681 ttggtcatca gaccggaaat ggtcgccact tcgaattcga aacctgcagc atggagatga
    16741 tacaacggca gcaacgtttc aatcggatgg ttaccggtcg aaaacagttt gccgttatcg
    16801 gtcggcaaat aacgttcgtc cgcggcgatc accagaattt tatgtttacc acgataaggt
    16861 tttgggtagt ccacgccatc aagatcagag acaggactag tatattggct aagcgaatat
    16921 tctgaaggga agaatgcatt atcttcagca atatcgacct gcggattttt acttgtttga
    16981 acagtcatag tgtattcctt attgttgctt agttagggta gtcactatat ttgcgactga
    17041 gctggtcgct atttttaacc aggctggaaa attgagtcaa tatatcgaaa gcggtagtcg
    17101 gcgggtgtaa gtatgtaatg atgatgctgc actggtgttg aatattattc acgccaaatt
    17161 ttccttattg agcaacgtcg tcgcaagtgg cctcaatgat tttagaccat tatcagaggt
    17221 ccggggcggc agggagagat ttagtgctgt ggaatattgt ggtgctgatg aatatttata
    17281 tcccggattg gtagagaaaa agctttttcc atgcactttt tttgcaaata tttctgtatg
    17341 ggaggcggtt ttgttgcaga ttaattcata aagaaatgca tttgggtgtt ggctggaaga
    17401 gtcgcgggta gatgttattg tgccagcagg ggcagggtaa ggtactgtat cgatatgctt
    17461 tggcaattgc atgtgccgca gagggagatg aaatgccaat aaaaaagcct gagttcgatg
    17521 agggacgaac tcaggctagt gttaagtttt acagtaacac gccgcagcga gttggctgat
    17581 tagaactggt agacgatacc aactgcaaac tggtcatcag ctgctgcgcc agtaatggcg
    17641 gcgtcatctt tatccagcat gttgaatttg tatgcagtat aaacgttcat gttcttatta
    17701 aagtagtacc aggtaccaac ttcaatgtag ttcacacggt cagcgtcaac atatctatgt
    17761 ccaggtaccc ctttgatatc tttaccttta gagtacacat aacctaagga cggacgcaga
    17821 ccaaagtcga attgatactg aacaaccgct tcaaagttct gggtttcatt gataagttga
    17881 tcacgcatta ttgtattgcc ttctcgatcg gcaacacctg tatcaataga acccggagtc
    17941 atattacgag tttcgccgta cattacagct gcgtagacat tgttcgcgtc atactttgca
    18001 gccaatgacc acagttcagc atttgaaccg tttccatcgg caacttgatc gttggtgcga
    18061 tcagatttgc tatatgcacc aacgatacca aaaccattga tgttatagtc tacagacaac
    18121 gcatagccgt caccattttg tttcttagtg ctacggttgc tgtttttgcc ttggtactga
    18181 atcgcaaaat caagaccatc aaccaggcca aagaaatcat agttacggta ggttgcaaca
    18241 ccgttggtac gaccattcat gaagttgtca gcggaagccc aggaatcgcc accccattca
    18301 actaacatat cggtataagc tgcggcatca taagcaacac cgtagttacg gccatagtca
    18361 aatgatccta actcgttata tttcaaaccg gcgaaggtta aacgggtttt ttcctggttt
    18421 tcaccttcgg gcttgctggc gtcgaggttg tattcaaact gaccaaaacc agtcagttcg
    18481 ctggtgattt gggtttcacc tttaacaccg aaacgagcat aggaggtatc ttcgttttcg
    18541 ctgttattat catcagtatt tgaccagatg cgttcgccaa ccatcttgcc gtagaaatca
    18601 actttattgc catctttatt ataaatttca gccgcatttg ctgcgccagc aactaataac
    18661 gccgggacca gcattgccag aacttttctt ttcattatgt attcccttgt gattataatc
    18721 ttcatgaata tatcaataag tgccgttatc caaaaaaaag cacatttgga tactattcta
    18781 tgaagttcat tttattttaa agattacatg taacaa
//
LOCUS       DAOTRK010000092        18488 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.092, whole genome shotgun sequence.
ACCESSION   DAOTRK010000092 DAOTRK010000000
VERSION     DAOTRK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.092"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(4..3483)
                     /locus_tag="QIE41_003044"
     CDS             complement(4..3483)
                     /locus_tag="QIE41_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDS3531907.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFLAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNARGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHERENNDIYSSCRLI
                     VRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            complement(3544..4191)
                     /locus_tag="QIE41_003045"
     CDS             complement(3544..4191)
                     /locus_tag="QIE41_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDS3531908.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPSFRQKLNEGWYQVRISGRDAGENELSARLNEPLANGAVIHVVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(4089..4832)
                     /locus_tag="QIE41_003046"
     CDS             complement(4089..4832)
                     /locus_tag="QIE41_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3531909.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     EYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVHSDLPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            complement(4838..5536)
                     /locus_tag="QIE41_003047"
     CDS             complement(4838..5536)
                     /locus_tag="QIE41_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDS3531910.1"
                     /translation="MQDIQQETLNECTKTEQSALIVLWEVDLTEVGGERYFFCNEQNE
                     KGESVTWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWIYRGDECGYDGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGYLSINKLSQ"
     gene            complement(5536..5892)
                     /locus_tag="QIE41_003048"
     CDS             complement(5536..5892)
                     /locus_tag="QIE41_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3531911.1"
                     /translation="MEDCTVPEPEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGG
                     LNADLKTFKPVFRVTDEYSRAALDSFLSRHAGIRAFLWRPPKHNRTVRVVCREWSISD
                     NAMYTDFNCTFEEVTH"
     gene            complement(5870..9097)
                     /locus_tag="QIE41_003049"
     CDS             complement(5870..9097)
                     /locus_tag="QIE41_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDS3531912.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQVSQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNSVNESGDRVIRALAAGMGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLSGVLNGLAGNIDTVATAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVVAARGTERQAAAEAKLTAAQASLTRNIAART
                     AAQTTLNTVTSVGSRLLSGALGLVGGVPGLVMLGATAWYTMYQNQEQARESARQYAAT
                     IDEIRQKTSAMSLPEASDNEEKTRQALDEQNRLIDEQKSKIKSLQEKIAGYQYVLANP
                     GWTTDNGFMINHMTSVKTVTEGLAEATNQLAVEQSRLTQMQGKAQSIQDVLAGLEERR
                     VALIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQTALNNSERELALSRLKGEARERARLGYAADDLGFVGEAYQTARQNYINNSLDAW
                     RNNQANKPKAHKKTEAEKTEDIYKRLIKQQKEQIALAGQNTELAKMKYQVSQGELSTL
                     SEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASARASDDAQLLGYGEGSRMRER
                     LQEMWSIRHEFEQKNNELLRQYQAGEIEEALWKQEKELNKKYLEERLSDQQDYYAKAD
                     ALRNNWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVVDWRNWG
                     SSVLREVSKILMNAAIVNGLKSLSGAGGWLGTVGGWISGAVANAKGGVYTSANLSAYS
                     NTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNSGGGVVYSPVY
                     HISIQNQGSNGEIDARSARGLVDLIDSRVVSIMQSSRRDGGLYSA"
     gene            complement(9144..9422)
                     /locus_tag="QIE41_003050"
     CDS             complement(9144..9422)
                     /locus_tag="QIE41_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDS3531913.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERADFRA
                     AQIVSAVYGAQGVKVSLNDALLQWEKEQTEGASDPFAGLENALLIVSQ"
     gene            complement(9446..9817)
                     /locus_tag="QIE41_003051"
     CDS             complement(9446..9817)
                     /locus_tag="QIE41_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HDS3531914.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(9832..10536)
                     /locus_tag="QIE41_003052"
     CDS             complement(9832..10536)
                     /locus_tag="QIE41_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HDS3531915.1"
                     /translation="MSSNFERSQLTKIMISSAPVTAETLDSASYLGLSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSAGTNGVVAATFSLRLKGKPVLTTASLKVKVDLKSTLRVA
                     SGAKLEMAVEAAGGVPPYSYVWKKGGSPVSGQTAATFSKASASSGDAGAYTCEISDSA
                     SPVNKVTSTSCTVTVS"
     gene            complement(10596..10940)
                     /locus_tag="QIE41_003053"
     CDS             complement(10596..10940)
                     /locus_tag="QIE41_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDS3531916.1"
                     /translation="MREATLYSLLSQLAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TASDVLDGQAESRITVQIDVWATVPDDADNIREQALDAVRKLAPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(10937..11386)
                     /locus_tag="QIE41_003054"
     CDS             complement(10937..11386)
                     /locus_tag="QIE41_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDS3531917.1"
                     /translation="MMDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDA
                     VVERAPERTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAF
                     YWRFVELGTINMPAHPFIRPAFDTTEELAAQVAIQRMNQAIDEVLSK"
     gene            complement(11383..11721)
                     /locus_tag="QIE41_003055"
     CDS             complement(11383..11721)
                     /locus_tag="QIE41_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDS3531918.1"
                     /translation="MQAGRLRDRVIILNVTTARSPSGHPVETVTEGATVWAEVKGISG
                     REIISGGAETAQATVRVWMRFRRDVTATSRLKVLTGAFKGAILGIEGPPIPDARATRL
                     EILCSLKGNV"
     gene            complement(11730..12047)
                     /locus_tag="QIE41_003056"
     CDS             complement(11730..12047)
                     /locus_tag="QIE41_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDS3531919.1"
                     /translation="MAAIVEKLRAQCRIDTDDATDDELLMLYFRAACRKAENFINRKL
                     YEETVPEGDPEGVLIADDVLLALMLLVGHWHENRENSSDVSKAPVPFGFSSLLEPYRF
                     IPL"
     gene            complement(12124..13341)
                     /locus_tag="QIE41_003057"
     CDS             complement(12124..13341)
                     /locus_tag="QIE41_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDS3531920.1"
                     /translation="MKLHEMKQKRNTIAKDMRALHEKIGDNAWTDEQRAEWNRAKAEL
                     DALDEQIAREEELRRQDQAYVDESGPEERQNNEAENGKKAVEEKRAAAFNRFLRAGFA
                     ELNAEERNLMRELRAQSVTTDSQGGYTVPTQMRNKIIDTMKAYGGIASVAQLLTTSTG
                     QDITWSTSDGTTEEGELLAENTAATEQDVTFGTAILGAKKLSSKIIRVSNELLQDSGV
                     DIESYLANRIAQRIGRGEAKYLVQGTGTGSPLQPKGLAASVTGTIQTAASAAFTWKEM
                     NALKHAIDPAYRGGPKYRWAFNDATLQTIEEMEDGQKRPLWLPDIAGGTPATVLGIPY
                     VIDQAIDGIGTGKKFIFLGDFNRFIIRRVTYMELKRLVERYAEFDQVAFLAFHRFDCV
                     LEDVAAIKALTGK"
     gene            complement(13356..13955)
                     /locus_tag="QIE41_003058"
     CDS             complement(13356..13955)
                     /locus_tag="QIE41_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDS3531921.1"
                     /translation="MNDRETRCYSGEVRAEQYDNAPTHILGYGSVFNSRSEPLWGFRE
                     IIKPGAFDDVLNDDVRGLFNHDPNFILGRSSAGTLSLSVDERGLRYDIVAPDTPTICD
                     LVLSPMLRGDINQSSFAFRVARDGESWYEDDEGIVIREITRISRLYDVSPVTYPAYQD
                     ADSGVRSMKAWQEARASGALKKAVNERMARERLLTLLNA"
     gene            complement(13948..15174)
                     /locus_tag="QIE41_003059"
     CDS             complement(13948..15174)
                     /locus_tag="QIE41_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDS3531922.1"
                     /translation="MLLDALFRSEPLENPSVPVTGEAAETDNIFARDVYVSPETSMKL
                     AAVYACIYVISSSVAQMPLHVMRKTNEHVQPARDHPLFWLVHDEPNAWQTSYKWRELK
                     QRHVLGWGNGYTWVKRNRRGEVTSLECCMPWETTLLNTGGRHTYGVYNEEGAFAVSPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQQYTSAFFNGNARPAGIISVKNELNE
                     QSWGRLKNMWQRAVTALRSQENKTMLLPAQLDYRALTVSPVDAQIIDMTKLNRSMIAG
                     IFNVPAHMINDLEKATFSNITQQAIQFVRYTMMPWVANWEQELNRRLFTRTERAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVDGLDEMLVSVNAANP
                     LNNFKDTKGKEEKNDE"
     gene            complement(15164..15325)
                     /locus_tag="QIE41_003060"
     CDS             complement(15164..15325)
                     /locus_tag="QIE41_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000811487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531923.1"
                     /translation="MKQLITDMTGLIGFGLLTAGVYLYAGLPASLMLSGCLLLLYALV
                     VSMRRKHAS"
     gene            complement(15322..17079)
                     /locus_tag="QIE41_003061"
     CDS             complement(15322..17079)
                     /locus_tag="QIE41_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDS3531924.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPQHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVALFTAMSRFLVNG
                     GGVNDFLSTLDPDEDLLIL"
     gene            complement(17079..17561)
                     /locus_tag="QIE41_003062"
     CDS             complement(17079..17561)
                     /locus_tag="QIE41_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDS3531925.1"
                     /translation="MSGPLKTPPRLHLIRGNPSKRPVKDPKKTAKKDEKCLPKIPQHL
                     GSQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNTAGPDDVDPLAELLKARD
                     "
     gene            complement(17709..18059)
                     /locus_tag="QIE41_003063"
     CDS             complement(17709..18059)
                     /locus_tag="QIE41_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDS3531926.1"
                     /translation="MPPRIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
BASE COUNT         4167 a         5552 c         4267 g         4502 t
ORIGIN      
        1 aattcagcta atcgtgatgc ctgcagtggc tttcttcatc accacaacca gcaaatcgct
       61 gatacttgct gtgggatacc agttatttac cagccatgct gacaccgaaa actccagtgt
      121 catgtgaccg tgaccggcag gcatatcaat aacaccactg taaatcaacg tattatccag
      181 cgcggtacgg ttataaattt cagcaccgtt tttccgtact atcaggcggc atgacgaata
      241 aatatcgtta ttctcccgct catgtttagc gccgctgaat gccaccgccg gaataacaat
      301 ctgccggtca aacggctgat cgtcataaac cctgacggtg atggtccctg atggccaccg
      361 ttccggtgca cgggagtccc gggggaaagc tttgcccact gttttaacga gatcgccttc
      421 aatctggttg gcggacaatt ttcccagaac ccgacagttc tcgttaatcg tgacgttgtt
      481 gagcgtcccg gagttcgcat tcacgttacc gctgatatcg gcatttttcg ccgtcagccg
      541 cccgtccggt gtcagggaaa atgccggagg attaccgccg ctggtaatgg tcggagccgt
      601 caggcgtttc aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg
      661 ctttgtgttg ccattcgcag gattaatcat cgcaatccgg tcagcagcca gaagcacctg
      721 actctgcata cctgctggcg tattctcaat accggctccg atacccgcaa tataaaggcg
      781 tccgtccttc atctgctgca gtttcacggc ccacatgctg ttcaggttat tatttgtatc
      841 aacctgaact ttctgtatct gctggattgc cgcactctgg tcttccagtt ttttattgac
      901 ggtctgcgtg atttcattgc tgacattcgt aatggacgtc ctgatttcag ccaggtccgg
      961 cgcaagctga ccgttatcaa tctgcgtcca caactcctgg gccagatgtg ttttcccgat
     1021 ttctcctttg aaaaaatcca ggtaaccttc cgcatcatcg ctcgcccgac cgacagcctc
     1081 cacgaatgcc gatttgccaa cggtgttcac actgcgaacg taaaaataat aatcatggcc
     1141 cggtttgata ttgatactgg cggctatcca gtacagcgcc gtgccaagat agcgggctgt
     1201 ggtttcaacc tgcctgatat cggtaatccg cttttccgag aaccagaact caaactgtac
     1261 cgtcgggtca taaacagcaa gatgcggcgt ggcggttatc tgaaaatagc ccggcgtcag
     1321 ctcaatcctc gacggtgctg ccggtgcggc aatccggaac gataccgacg ccggatcgcc
     1381 ctgctgcccc cgggcattta ccgcccggac tgtcagcctg tagttcccca gcgccagttg
     1441 cgtgaagcgg tatgtggttt ccgtcgtccg ggccgtgctg accagtcgct cactgccgtc
     1501 gtccgctgtt acggtcagac ggagcaggaa gctcacgccc ttcaccacct tcggtgtgtc
     1561 ccatcgcgcc agcacctgat attccccgct gtctgcagtg acttcggcgg tcaggtgctg
     1621 cactgctggc ggcgtgacac cattcaccgt gccgctctgg tcaccgtcaa agtgcgcccc
     1681 gttatccacg atggcttctt tctccggtac atgctgcacg gcggtgatgg catacgtgcc
     1741 gtcatcgttc tcacggatac tcacgcagcg gaacaggcgc tggcgcagcg tcggcagctt
     1801 cagcccccac acgctgtatt ctgcaacgcc gtcaggaaca cggctcactt tcaccttcac
     1861 gccgtcggtg acggactgga cctccacgct gaccggatta ccctgaccgt caaccaggct
     1921 tatcagcgtg gtaccggagg atggcagcgt gatttcacgg tcgagcgtca gcgtccgggt
     1981 ctggctgttc accgccagca cgcgcccgcc ggtgctgata cccgcatagt catcatcgca
     2041 gatttcaatg acatcccccg gtacatggcg aagcccttcc gcacccacgc tgaagtccac
     2101 ggtctgcgtt tccagcagtt ccgttttaat cagccacagc ccggcgcggt gcgcctgccc
     2161 ccggctggta cagccaaagg catccatctt cgtgacgtta cgaccgtaac gggcaatggc
     2221 ctgcgtgtcc tccacaagct ctgtcgccgt ctcccagccg ttatccgggt caatccagtt
     2281 cacctcaacg gcattatggc ggtctttcag ggcgctgaaa ctgtagcgga acggcgcgcc
     2341 atcatccggc atcaccacat tactgcggtt ataggcccac accttatccg acggtcggtc
     2401 ctgcacgaac gtcagcgtct gcccgttcca taccggcata cagcgcatcg ccgagcagaa
     2461 atcactgagc acatcccacg ccttgcgctg tgtggtcagg tacgcattac aggtgatgcg
     2521 cggctccgtg ccgccaaagc cgtccggcac tgactggtcg cagtactggc cgatgacata
     2581 cagcgcccat ttgtccacat ccgccgcacc aagacgtttt cccatgccgt agcgcgggtg
     2641 ggtcagcata tcccacagac accaggccat gttgttgctg tatgccggtt ttaacgttcc
     2701 gtcccagata ccgctgtatt gtcgcgtctg cgggttatag ttcgacggca cctgcagaat
     2761 gcgcccgcga agatgataat tacgactcac ctgctggctg ccgaactgct ccgagtccac
     2821 ctgtacgccg accagtgccg tgttcgggta gcactgtttc acatcgatga tttcggtgta
     2881 tgacgaccag agcgttttgt tctgcagctg gtctgtggtg ctgtccggcg tcatcctgcg
     2941 catccggata ttaaacgggc gcggcggcag gttatccacc accaccgagg ccagatactg
     3001 tgaagtggtt ttacccttaa tggtgatgtc tttttccgtc acccagccac cgttacgttg
     3061 tatctgaacc agcaggcgga cttccgatgg attcctgtcc ccctttgagg tggtttccac
     3121 cagtgcctgc acgccgaaag taaaacgcag acggtcgatg tttgccgacg tgatggtccg
     3181 ggtgatcggc gtgtcatatt tcacttctgt accgagcacc gtctcggagc cggaggattc
     3241 aaatccctcc ggcggtgtct gctcctgctc accggccagg aacaccaccg tgacgccgga
     3301 tatattggta ttcccctcac tgtccagcac cggcgtactg ttcagcagca cgctttttaa
     3361 tccatccacc ggaccttcaa ccggcccttc gctgatggca tcgatcacac tcagcagctg
     3421 cgtggacttc aggttgtcct tcgcttcgcg cggggtatgc cccttactgc tgcctttacc
     3481 cattcgtcac gctccataaa cgacaaaacc gcccgcaggc ggtttcacat aaaatgtttt
     3541 gcatcagcga ccaatcacca caacctgacc accatcccct tcgtctgccg tgctgatctc
     3601 ctgagaaacc acacgtgatc ccacgcgcat ttccccgtac agaacaggca gaacattgcc
     3661 ctgggcaacc atgttatcca gtgacgagaa atacgtgttc tgtttgccgt tatctgtact
     3721 ggctgccgtg ggcgtcctgg ctttcggtgc cagcatctgc gccacaccac ccaggatcat
     3781 actggcccct gctgcataca tacccgatac agccgcggcc cccagccagc caacagggtt
     3841 ccaccatgcc accgcaatca gcgccgcacc cagcaccgcc tgaaacacac cgccactttt
     3901 ggcacccgcc agacgcggaa cgacgtggat cacggcacca tttgccagcg gttcattaag
     3961 acgggcagat aattcatttt cgcctgcatc acgcccggaa atgcgcacct gataccagcc
     4021 ctcattcagt ttctggcgaa acgacggaag ctgtgtggcc agcgcccgga tggcttcagc
     4081 ccccgttttc acacgaaggt cgatgcggcg gccaaatcgt tgtaaatccc cgtaaaggca
     4141 gatgcgtgcc atgcccggtg acgccagagg gagtgtgtgc gtcgctgcca tttgtcggta
     4201 tacctctctc gtttactcag ttgttcagga atatggtgca gcagctcgcc gtcgccgcag
     4261 taaatagcgg catgattcgg caccgatgaa ccaaaacagc acagcagcac atcgcccggc
     4321 tgcgccgctg acaacggcac ctgatatagc cccgttgcct ccagattatc cagatagaga
     4381 ttctgaccgt gacgccacca gtcatcctca cgatgaaaat ccggcatctc aatccccgcc
     4441 agatggtaag catcccggaa cagcgtgtaa cagtccgtca ccccgtgctc aaagcgccgc
     4501 ccggtgagat gcggcacaca gcggaattta tgaatctcac cccggcagac cagccaccac
     4561 ggcaaatcac tatgcacctg cagccgccgg tcggcctcac tcagccaggg cagaccaccg
     4621 gggtggctgt ggaccagcgc cacaatctca ccctgcatct ctgcccgcag ccagtcctcc
     4681 ggcgacatcc ggaaatactc ctccggctca ccggagatat tcacgcaggg aaaatatctt
     4741 tccccttccg gcgttctcac cacgaagccg cacgactccg ctggcgcaca tcgccgggcg
     4801 tgcgccagaa tcgctgattc tgtctctgtc atgggattta ctgcgaaagt ttgttaatgg
     4861 aaaggtagcc gccaaaattg ccgacgttat tgcggaactt gcaaccactc aggcatttgc
     4921 tgcacttatc cttcgtgata tcggacgttg gctggtcata ttcatccgcg acagccgggc
     4981 catcataacc gcactcatcg ccgcggtaga tccaggtgca ggtgttggcc agcatgatgc
     5041 gccccggaaa aacggcacca tccgtttccg tcggtgtgga caacacaaag gaggcactga
     5101 ccgcgctcag ttcgctgcac tgctcgatgc gccagcggct gatcacctcc tgctccggat
     5161 cggcgtcact gtttccgttg acgaagttca ccgcatccag aaaacgggcg taaaccttac
     5221 gcctgaccac cgttccgccg accagactct gcaggtcttc cgccatcccg gtgaccatgc
     5281 cgtgcaggtt agagactttc agcgttggtc ttgcactggc tcctttgccg ttcatctcaa
     5341 agccgcttcc ctgaataggg tacgcctgat actgtcgccc ctgccaagtg accgactcac
     5401 ctttttcgtt ctgctcatta cagaaaaaat aacgttctcc gccgacctct gtcagatcga
     5461 cttcccagag cacgatcagc gcggattgct ccgttttagt gcactcattg agtgtttcct
     5521 gctgtatatc ctgcatcagt gagtgacctc ttcaaaggta cagttaaaat cggtatacat
     5581 ggcattatcc gaaatgctcc actccctgca gacaacccgg acagtcctgt tgtgttttgg
     5641 cggacgccac aaaaaagcac gaatcccggc atgacgggat aaaaaactgt ccagcgcggc
     5701 acgggaatat tcatctgtga cacgaaatac cggtttaaac gttttcagat ccgcattcag
     5761 accaccagcc cgtcgctgtt catatccgtc accaaacttt accgtaataa ctgatggctt
     5821 tcgtgtcgtc tccatcccct cacgggggat ccagttaaaa acttcaggct caggcactgt
     5881 acaatcctcc atcccgacgc gatgactgca taattgacac aaccctgctg tcgatcagat
     5941 ccaccagtcc cctggctgag cgcgcatcta tctcgccatt gctcccttga ttctgaatgc
     6001 tgatgtgata cacgggagaa taaacaaccc ccccgccact attcacattg ccaatggctc
     6061 tgaccccaag agagccgtcc gctgcccgtg tcagtggcat gatagcttca ggcccggcct
     6121 cgcccatcag cccggcacct ttcgcaaaag caaaatacgt cggtgtatcc acaatagtgt
     6181 tactgtaagc actcagattt gccgatgtgt aaacaccacc ttttgcgttt gccaccgccc
     6241 ccgaaatcca tccgccgacc gtaccaagcc accctccggc accggagagt gacttcagtc
     6301 cgttaacaat ggccgcattc atcagaattt ttgaaacttc ccggagaact gaactccccc
     6361 agtttctcca gtccacaaca tttccggcca gtgcatcgga aatatttgat accagtccgt
     6421 ccatagtgga aacgacagca tctgccgcct gcgaagcata atcggtggca ctgtctgccc
     6481 agttggtcag cccctcctgg agtcctgcat tccagttatt acgtaaagca tcggcctttg
     6541 cataataatc ctgctgatcg ctgagacgct cttccagata ttttttattc agttctttct
     6601 cctgtttcca cagggcttct tcaatttctc cggcctgata ctgtctcaga agctcgttat
     6661 ttttctgctc aaactcatgc cggatactcc acatttcctg gagtcgttca cgcatccgtg
     6721 agccttcacc atatcccagt aactgcgcgt cgtcagatgc ccgggcactg gcattactgt
     6781 ccgccaggct gctttcatac gcagcaagct gctcacgaat ctttttctgg tcgatgagtg
     6841 ctgcattctg caaaagcgtt tttttctgcg cttctgacag ggttgataat tcgccctgac
     6901 tgacctgata tttcatctta gccagttcag tattctgccc tgccagtgct atttgttctt
     6961 tttgctgttt aatcagccgt ttataaatat cttctgtttt ttccgcttcg gtctttttat
     7021 gcgctttggg tttatttgcc tggttatttc gccaggcatc cagtgagtta ttgatataat
     7081 tctgtctggc tgtctgatac gcctctccca caaagccgag atcatccgca gcataaccca
     7141 ggcgggcacg ctcacgggct tcccccttca ggcgggacag agccagttcg cgctcgctgt
     7201 tattcagtgc agtctgctgt ttatcatcca gggttgcctg tggtagccgt aacggtacat
     7261 tcaccagccc ctgtcgctgc tgaagtaatt cattaccgag cccgagaagg cgattaaact
     7321 cggtatgctg cccattcatg atcaacaggg actgatacgc tttgttttgt tccgcggcct
     7381 gttgacggat caacgccacc cgtcgctcct ccagcccggc aagcacatcc tgaatggatt
     7441 gcgctttgcc ctgcatttgt gtgagacggg actgttcaac tgccagttga tttgttgctt
     7501 ctgcaagccc ttctgtgaca gtttttaccg acgtcatgtg gttaatcata aaaccgttat
     7561 cggttgtcca gcccgggttt gccagcacat actgatagcc agcaattttt tcctgtaagg
     7621 atttaatctt acttttctgc tcgtcaatta acctgttttg ctcatcaagt gcctgccgcg
     7681 tcttttcctc attatctgac gcttcaggaa gcgacattgc cgacgttttc tggcgaattt
     7741 cgtcgattgt tgcggcatac tggcgtgcag attctctggc ctgctcctga ttctgataca
     7801 tcgtgtacca ggccgtcgcc cccagcatga cgagtcccgg cacaccacca accaacccca
     7861 gcgcaccact taacagacga ctccccactg acgtgacagt attcagcgtt gtctgtgccg
     7921 ctgttctggc cgcaatatta cgggtaagtg acgcctgagc agctgtcagc ttcgcttctg
     7981 ctgcggcctg cctttcggta ccgcgagcag caacaaccgc ctgttgcgca cgataaaccg
     8041 ccgcacgcgc cctggcggtt gctatctgtg tcccccgaag ttgcgcttca gcaagagcca
     8101 cttcgtttct ggctgcagta attaatccgg cagttgcaga tccagcagac gacgccatat
     8161 tgccaaaata tcgggctacc ccgacggcaa ccagagcacc ggcagcggtt gccacggtat
     8221 caatattgcc tgcaagacca ttcagcaccc cggagagcgt cttcgtcact ccgcttgcct
     8281 cgttcgcacc accaacccag gccataaagg cgttttcaac tttggttgca gaggatgaaa
     8341 ccgtatcagg cattgctgca tattcatcac gcaacgcccc aagctgacta atcagtgcag
     8401 gaacaacctt atcggcggtt agttttccgt tatccgccat ggccttcaga tccttacggg
     8461 caacgcccat tcccgcagcc agcgcacgaa taacacgatc accgctctca ttcaccgagt
     8521 taaactcttc accgcgcagc actccctgcg ccagtgcctg actgaactgc gtgatcaccg
     8581 aactggcttc tgctgtactg gcaccggata atttcaggcc cgtggagatc gcctcggtga
     8641 ctttcagtac ctcctcagaa ctgtaaccat actcccgcat ggaagctgca gaacgggcaa
     8701 aaaggctggc gttatcagaa aacgccgtcc ccgttctctg gctgatcgcc attaattcac
     8761 gttgtgatac ctgaaaatca tcactggact gtgaggcctg cttcagacgg gcatttactg
     8821 aattccactc atcggcgaga gaaataagat gaccggtagc aaaagccccg gcaaatgccc
     8881 ccgccatatt cagtgccgaa gatttagctg tatttatctg atccgtcact tctgccagtg
     8941 cacgccgcat ttcacgggat gcagcagcgg actgccggcc tccgttctgc atggtacggt
     9001 agtaatcctg ccccatacgc gaagcccggg agatctctga ctggaatgac cgggaatttg
     9061 ccgagatttt aataatcagt tcacgtaatg tcgccacact cattctccgg acgaaaaaaa
     9121 accgccgaag cggttatgtt gactcactga gacactatta aaagcgcgtt ttccagtccg
     9181 gcaaatggat ctgaagcgcc ttctgtctgc tccttctccc actgaagaag cgcatcattc
     9241 agtgacactt tgaccccctg cgcaccgtaa acagctgaaa caatctgggc agcccggaaa
     9301 tcagcccgtt cgtcccccag cgggctgaac ctgtcaaatt ctgcccacat catgatttct
     9361 gatgcggaca tttcccggcg taactctgac aatgtgcgcc ccatcctgag cgccagcatc
     9421 atcagaaaac gcatccccgg aagcgctact tttttttaac ctcgccggca tcactgatca
     9481 gttccagaga ctgccgaaga agccgcgcat gcaccgggcc atacacggca atcacctgtt
     9541 cacgatcatc ctctgaaaat acgggttgca gtccggtatc acacagaaca tcaatgaaca
     9601 gttcaacatc tgcctccaga tttcggcggg cgcgctccgc aacggataac ggtgtctcat
     9661 catcttttgc tttaacgatc tcctgccagc gcaaccaggc ttctgcagaa ggttcccgta
     9721 acacaaccgt tgctccctcc cattcaggca catcaacggt tttatggcga aaccccgaca
     9781 tcgttgccag tgccagatta cggatatttt tagtcatcac atccatcctc attaactgac
     9841 ggttacagtg caggaagtgg aggtcacttt gttaaccggg ctcgctgaat cagaaatctc
     9901 gcaggtatac gcacccgcat caccggatga tgctgatgcc ttactgaacg ttgccgccgt
     9961 ctgtccggaa acaggagaac cacctttctt ccagacataa gaataaggcg gcacaccacc
    10021 cgcagcctca accgccattt caagtttcgc tccggaagca acccgcagcg tgctttttaa
    10081 atcaaccttc actttcagcg aagctgtcgt cagcacaggt ttgcctttca gacgcaggga
    10141 aaacgttgca gccacaacac cattggttcc tgcagaccag gtatgctgac gcacttctgc
    10201 cataaaggta aatccgttgc ctgacggaaa aataacttta aagccatacg tggtgtcatt
    10261 gtcataggca ctgcgcaacg cattctgggc agcattgagg taaaagttgc ctgacatgga
    10321 aatctctgac gcggcaccaa gaccgttaat attttcctgc tcaacagaac acagcgtggt
    10381 gacatcaata tcctgctttt gtccagcggt aaactgcact tctttgattg tacagctcag
    10441 gccaagatag ctggcagaat ccagggtttc tgctgttacc ggtgcagacg aaatcataat
    10501 tttcgtcagt tgcgaacgct caaaattaga ggacatactc gtctcctgaa aataaaaaac
    10561 ccgccagcgg cgggtgggta aaatcattaa cgacctcagg ctattacctg aaattcaagc
    10621 gtggctctgc tcagccggga atcaggatca taaccctgcg ttttagaaat aacggagggt
    10681 gccagtttcc ttaccgcatc aagcgcctgc tcacgaatat tatctgcgtc atcaggtact
    10741 gtcgcccaga catcgatctg cacggtaatt ctggattcag cctgcccatc aagcacatca
    10801 gatgccgtgt cagacaccac agaaaacacc agccacggcg gagataccgc aggctttccc
    10861 tccgtcagcg ggaccacata aggataaacc tgtcctccgg ccagctgaga caacagggaa
    10921 tacagtgtgg cctccctcat ttacttaaga cctcatcaat agcctgattc attcgctgta
    10981 tggcaacctg cgccgccagc tcctctgtcg tatcgaaagc cgggcgaatg aatggatgcg
    11041 cgggcatgtt tatcgttccc agctccacaa agcgccagta aaatgcattt cggggatcgc
    11101 tggctttcat gctgttatca ctgtttccgg ttcgcaggtt ccgtccgcga atgtggacac
    11161 ccgagataat ttccccccga cgctttgaac gctgagtgag aacaaccaca tttttcttca
    11221 gtttcccggt tcgctccggc gcacgttcaa caactgcatc ccgcataact tcagcaccgg
    11281 cacgggtggc atcgcgcaga accttattgt tttctgccct gctgagcgtc tccaaatccc
    11341 gtgcaatatc cgccaggccg gaaaaatcaa gactgaaatc catcacacat tccccttcag
    11401 gctgcagagt atttcaagcc gggtagcgcg tgcatccggt attggtggac cttctatacc
    11461 cagaatggcc cctttaaatg caccggtcag cactttcaga cgtgaagtcg ctgtcacatc
    11521 gcgccggaat ctcatccaga ctctgaccgt agcctgagcg gtttctgctc cgcctgagat
    11581 tatctccctc ccgctgatac ccttaacttc tgcccatacg gtagctccct ccgtcaccgt
    11641 ctccaccgga tgccctgacg gagagcgggc ggtggtgaca ttcagaataa ttacgcgatc
    11701 acgtaatctg cccgcctgca tctctcctcc tacaaaggaa taaaacgata aggctccagc
    11761 agagaagaaa aaccaaacgg gactggtgcc ttgctgacat ctgaggaatt ttcccggttt
    11821 tcgtgccagt gcccgaccag caacatgagc gccagcaaaa catcatcagc tataagcacc
    11881 ccttcaggat caccttccgg caccgtctcc tcataaagct tacggttgat aaaattttct
    11941 gccttgcggc aggcagcccg gaaatacagc atcagtaact catcatcagt tgcatcatct
    12001 gtatcaatac ggcactgcgc cctgagtttt tccactattg ctgccatcag aaactcctgc
    12061 ccgcaacact gtgcgggcat aaaaaaaccg cgtcggcgcg gtctgtaact gaacaacgag
    12121 tggttatttg ccagtgagcg ccttgatggc tgccacatct tccagcacac agtcaaaacg
    12181 atggaaagcc agaaatgcca cctgatcaaa ctcagcataa cgctcaacca gacgtttcag
    12241 ttccatataa gtaacgcggc gaataataaa gcggttgaaa tcccccagga aaatgaattt
    12301 ttttccggta ccaatcccgt caatagcctg atcaataaca taagggatcc ccagcacagt
    12361 agccggcgta ccgcctgcaa tatccggcag ccataacggg cgtttctgtc catcctccat
    12421 ctcttcaata gtctgcaatg tggcatcatt gaatgcccag cggtatttcg gcccaccacg
    12481 atatgccgga tcaatagcat gtttcagggc attcatttct ttccaggtga aagcggcaga
    12541 ggctgcagtc tggatggttc ccgtcaccga cgctgccagc ccttttggct gtaacggtga
    12601 tcccgttccg gtcccctgaa ccagatattt tgcctctcca cgaccaatac gctgggcaat
    12661 acggtttgcc agataagatt caatatccac cccactgtcc tggagcagct cattggacac
    12721 acgaattatt tttgatgaca gctttttagc ccccagaata gcggtcccga acgtcacatc
    12781 ctgttccgtt gcggctgtat tctccgccag cagttcgccc tcttcagtcg tgccatcaga
    12841 cgttgaccag gtgatatcct gcccggttga tgtggtcaga agttgcgcca cactggcaat
    12901 cccgccataa gccttcatgg tgtcaatgat tttgttacgc atctgcgtgg gcaccgtata
    12961 tccgccctga gaatccgttg ttacactctg agcccgcagt tcacgcatca gattacgctc
    13021 ttcagcattc agttctgcaa atccggcacg cagaaaacgg ttaaatgccg cagcgcgctt
    13081 ctcttccacc gcctttttcc cgttctccgc ctcattattc tggcgctctt ccggcccgga
    13141 ctcatccaca tatgcctgat cctgacggcg caactcttct tcacgggcga tttgctcatc
    13201 cagcgcatcc agctcagctt tcgccctgtt ccactctgcc cgttgctcat cagtccatgc
    13261 gttatcacca attttttcat gcagtgcacg catatccttt gcaatggtgt ttcgtttttg
    13321 cttcatctca tgaagtttca tcgtcagtag tatccttatg cattaagaag ggtcaaaaga
    13381 cgctcacgcg ccattcgttc gttaacagct ttcttcagcg caccactcgc ccgcgcttcc
    13441 tgccaggctt tcattgagcg gacaccagag tctgcgtcct gataggccgg atatgtcacc
    13501 gggctgacgt catacagacg agaaatgcgc gtgatttccc ggataacaat cccctcgtcg
    13561 tcttcatacc agctctctcc gtcacgggcg acacgaaacg cgaacgagga ctgattaatg
    13621 tcaccacgca acattggaga cagcaccagg tcacaaatag tcggagtatc cggtgcaaca
    13681 atgtcataac gtaaaccgcg ttcatccacc gacaatgaca acgtgccggc agaacttcgt
    13741 ccgagaatga aattaggatc atgattaaac aagccacgta catcatcatt cagtacatca
    13801 tcaaaagccc ccggcttgat gatttcacga aatccccaca gaggttctga acgactgtta
    13861 aataccgagc cataccccag aatgtgggtc ggggcattat catattgttc cgcccgcacc
    13921 tccccgctgt aacagcgcgt ttcacggtca ttcatcgttc ttttcctctt tgcctttcgt
    13981 atctttaaag ttattcaacg gatttgctgc atttacgctg accagcattt catccagacc
    14041 gtcaaccggg ttcatatcct caaatgccct ggcttcattc cgactcatcc agccatctgt
    14101 aatggcaaag tgatagaact gcgcacgctc ctgtggggtc ccacggagca accccgtgag
    14161 gttgaaacga acgtaatacc cggcagcccg ttctgtacgg gtaaacaggc gacggttaag
    14221 ctcctgctcc cagttcgcaa cccagggcat catcgtgtag cgaacaaact gaatcgcctg
    14281 ctgcgtaata ttcgaaaatg tggctttttc caggtcatta atcatgtgcg ccgggacatt
    14341 aaaaatcccg gcaatcatcg accggttcag cttggtcata tcaatgatct gagcatccac
    14401 cggagaaact gtcagagcgc ggtaatccag ttgcgcaggc agcaacatgg ttttattttc
    14461 ctggctgcga agcgctgtca ccgcccgctg ccacatattt ttaagcctgc cccagctttg
    14521 ttcgttcagt tcatttttca cagaaataat accggcagga cgggcattac cgttaaaaaa
    14581 ggcgctggta tactgctggc cactcattcc cataccaatg gtttcagcat gctgcatgat
    14641 cgggctcagt cccattttct gattgtttcc cagcgccctg atatggatca tgtcgtccgg
    14701 acttaccgca aatgcaccct cttcgttata caccccgtaa gtatgacgcc caccggtgtt
    14761 aagtaacgta gtttcccatg gcatacagca ttcaaggctg gtaacctctc cacgacgatt
    14821 acgttttacc cacgtataac cattgcccca ccccagcaca tgacgctgct tcagttcccg
    14881 ccacttatag ctggtctgcc aggcattcgg ttcatcatga acgagccaga acaacggatg
    14941 atcgcgtgca ggctgaacat gctcattcgt ttttcgcatc acatgcaggg gcatctgagc
    15001 cacactggat gaaataacat aaatacaggc atagacagca gccagcttca tggatgtttc
    15061 cggactgaca tacacatccc gggcaaaaat attatccgtc tcagcggcct ctccggttac
    15121 cggaaccgag ggattttcca gaggctcact gcgaaacaga gcatcaagaa gcatgttttc
    15181 tcctcatgga caccaccagt gcataaagca gcaacaaaca gcccgacagc atcagagacg
    15241 ctggcagacc tgcatacaga taaacgccag cagtgagcaa accgaaaccg atcagcccgg
    15301 tcatatcagt aataagctgt ttcacagaat taacaggtcc tcatcaggat caagcgtgga
    15361 cagaaagtca ttcacgcccc cgccatttac cagaaagcgg ctcatggccg taaaaagtgc
    15421 aacagggccg tcgattttgg cttcaggcgt ggatttattc gggaagatgt tgtcgttttt
    15481 gtccggtttt accgtaacgt tagacatcat ccagttcatg accggatgat tgctgtgatg
    15541 gaaacgcccg gcataaacca gtgattccgt ttccttcatg gcctctgaca gattgcggac
    15601 cgtctgcgga acctccacca gcggtatccc ttcttcagcc agtgccaggc tgaactgcat
    15661 tgcgctccac gggtcaaatc ccagttccct gaggttttca ccgccaatcc attccagtaa
    15721 gtcacttttt atctgagcat gatcgataac atcaccatcc gtcaggatga gcttatccat
    15781 ctccgcccac ttccggtaaa gttctgcctg ctgccgcgag catcgctcca gccgtccttc
    15841 cggaagccag aatttaaaat cagcatgaac atgtccgtta tcggttcgcc agagttttgc
    15901 cgccgcacag atatcaatct tatgagcaag gtctacgccg acccacatgg gatatgtttt
    15961 cagctcatgt tgtggagcaa tgtattcgca cttctcccac ttaatcatat ccatccaggc
    16021 agattcggca gtgacccaca cattcatgtg tttggtaaaa aaattcaccc gcgcagagac
    16081 ctgctccttc gcttttttcg ccagacgacg cagatcatcc cagcgtttac agatgcccag
    16141 gccaggattc gctttctgcc agaccgtttc atcaaacgga tcatctccct catcgagcgt
    16201 gtaaatgatc gcaaagtagg agtcgtcttt taccgcgccc tccacgtcgc tgttatagcc
    16261 tcgcaatacc ttaatggcgt aatcgcgctg ctcgtagcaa atcccttcct tgttaaagcc
    16321 agccgtggtg atgccaaata acagagactg cagacgggca ccggttgccg tttccagaac
    16381 gtcccacacg tcacgggttt tatgagcatg cagctcatca ataatggcgc agtggatgtt
    16441 cagaccgtcc aggttgtttg catccgagga aagcggttca aattttgatg cgctctgctc
    16501 ctggtaaatc gccagcttgt tgaagtcaaa caaccgcccg agtgtcgacc gggcttttct
    16561 gaccatattt ttggcgtctt caaacacgat tctggcctga tcacgcgtgg ttgcggctga
    16621 atacacctca gcaccacctt caccatctgc ccccgtcata tacagaccga tacccgatga
    16681 cagggttgat tttgcgtttt tacgggcgac ttcgttgtac gccgtccgga accggcgcac
    16741 catcaccggg cgtccgctgc catcgctgcg catgacaact tccccggtct cttcattcac
    16801 cagcggaatg acaaaaccaa aaatattaat gaggataaat acatgccagt ccatcagttc
    16861 aatgggctgg cctgccagcg cccctttcac atggggcaca aatttgtaga aattaaggat
    16921 gtgctgcgca cggggttcac taaaataaat cccccgctct tcgccgtact tcagatcatc
    16981 aagaaaacgc tggcaggcca gacggacaaa ttcgccagcg acaatttctc ctgcaacaac
    17041 acgttcggcg tagcggatcc cgtcagccac ttttgccatc agtctctcgc ttttaaaagc
    17101 tccgccagcg gatcaacatc atccggtccg gcggtattta ctttcgcccg gcttgccggt
    17161 gacataccaa attctgcaag catcgcccgg atccgcttcc aggcatccgc tttcatcgca
    17221 gcagccgggt gtgccttaat cagcacatca ccgttctgcg tttccgtgcg gtaggtatac
    17281 ccctcaacat cgagtgtttc gcagtgatgt cggtattcgg tgtaggcttc caccagtaac
    17341 tcgagtgcac gcgcatcaag ctgagaaatg atcccttccg cattcagttc ttccgccatt
    17401 cgcctgaacc agtacttccc ctgcgaccct aaatgttgcg gaattttagg gagacatttt
    17461 tcatcctttt tagcggtttt ttttgggtct ttaacggggc gctttgaggg gttgcctcgt
    17521 atcaaatgca ggcgtggcgg ggttttcagg ggtcctgaca taatcggttt tacctatcaa
    17581 tcatttaatc acattccaaa aaaaagtttt cgaacctgcg gcgatgcgag gaaaggtcag
    17641 gcggcggtac tgagcagcca gggttgcaga gatttgacct gcccctcccc tacagatggg
    17701 aactgttatc aattgatgcg ttcgcgcgct gtttttgctt tatgacaggg ccagcacaga
    17761 ctctgcaggt tactgtctgc atccgtgcca ccatgagctt tcggaatgat gtggtccaca
    17821 gttctggctt caacggctct cccattgcgc aggcagttct gacacagatg attatcacgc
    17881 ttcagtatgc gcgcacgtat ggcatcccat ttcgagccat agccacgctg gtggcggctc
    17941 agtccgcgtt gatgctgtac ccatccttcg ccacgatgtt tatcgcagta accagaactg
    18001 tctgtggttg tacctgcaca tccacgctta cggcaggcgc gtgggattcg tgggggcata
    18061 tgtactccaa tgaagaagcc accgacatag cctcctccat tcatagtgaa actattttca
    18121 tctacccagt aatgaattct ttgtagagtt gtgatcaata caactcacta atggagaggc
    18181 ttgtccaaca cgttggacaa gtttcctgtt tgatttactg gacactatag aaggacagaa
    18241 tgccttcatc actcgaataa catcaattaa ggaggttcaa catgtttcat tccacaaatc
    18301 atcaggctgt aattatggct gcatcagctt gtgccacaga ccttttccgc ttcactttga
    18361 gcctgattca tttctacctg accggctcgc ctctatcttt ttaatccccg ctttatccaa
    18421 attgcattgc cagaatgccg acaacagact gacattcaaa tcctgactac ctccgatagg
    18481 ctgagcat
//
LOCUS       DAOTRK010000093        18225 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.093, whole genome shotgun sequence.
ACCESSION   DAOTRK010000093 DAOTRK010000000
VERSION     DAOTRK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.093"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..743)
                     /gene="aroD"
                     /locus_tag="QIE41_003064"
     CDS             complement(<1..743)
                     /gene="aroD"
                     /locus_tag="QIE41_003064"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDS3531927.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVDSVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLT"
     gene            complement(774..1640)
                     /gene="ydiB"
                     /locus_tag="QIE41_003065"
     CDS             complement(774..1640)
                     /gene="ydiB"
                     /locus_tag="QIE41_003065"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDS3531928.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1652..2917)
                     /locus_tag="QIE41_003066"
     CDS             complement(1652..2917)
                     /locus_tag="QIE41_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3531929.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIALDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3144..4358)
                     /locus_tag="QIE41_003067"
     CDS             complement(3144..4358)
                     /locus_tag="QIE41_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3531930.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRATT
                     LLMLYTFISFTALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(4457..4813)
                     /locus_tag="QIE41_003068"
     CDS             complement(4457..4813)
                     /locus_tag="QIE41_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDS3531931.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKYAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4949..5056)
                     /gene="rprA"
                     /locus_tag="QIE41_003069"
     ncRNA           complement(4949..5056)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="QIE41_003069"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5242..6354)
                     /gene="ydiK"
                     /locus_tag="QIE41_003070"
     CDS             complement(5242..6354)
                     /gene="ydiK"
                     /locus_tag="QIE41_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDS3531932.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVGQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGETEKPNK"
     gene            6743..9799
                     /locus_tag="QIE41_003071"
     CDS             6743..9799
                     /locus_tag="QIE41_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDS3531933.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCSEL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTYDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPVKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALSAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRVAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9796..10206
                     /gene="menI"
                     /locus_tag="QIE41_003072"
     CDS             9796..10206
                     /gene="menI"
                     /locus_tag="QIE41_003072"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDS3531934.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10306..10494
                     /locus_tag="QIE41_003073"
     CDS             10306..10494
                     /locus_tag="QIE41_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDS3531935.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11043..11411
                     /gene="sufA"
                     /locus_tag="QIE41_003074"
     CDS             11043..11411
                     /gene="sufA"
                     /locus_tag="QIE41_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDS3531936.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11420..12907
                     /gene="sufB"
                     /locus_tag="QIE41_003075"
     CDS             11420..12907
                     /gene="sufB"
                     /locus_tag="QIE41_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDS3531937.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGLFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGVVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12917..13663
                     /gene="sufC"
                     /locus_tag="QIE41_003076"
     CDS             12917..13663
                     /gene="sufC"
                     /locus_tag="QIE41_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDS3531938.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13638..14909
                     /gene="sufD"
                     /locus_tag="QIE41_003077"
     CDS             13638..14909
                     /gene="sufD"
                     /locus_tag="QIE41_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDS3531939.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTSDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAHSVTHIAVKRGQRPVKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14906..16126
                     /gene="sufS"
                     /locus_tag="QIE41_003078"
     CDS             14906..16126
                     /gene="sufS"
                     /locus_tag="QIE41_003078"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDS3531940.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGTKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16139..16555
                     /gene="sufE"
                     /locus_tag="QIE41_003079"
     CDS             16139..16555
                     /gene="sufE"
                     /locus_tag="QIE41_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDS3531941.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSS
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16704..17708
                     /gene="ldtE"
                     /locus_tag="QIE41_003080"
     CDS             16704..17708
                     /gene="ldtE"
                     /locus_tag="QIE41_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDS3531942.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17772..18008)
                     /gene="lpp"
                     /locus_tag="QIE41_003081"
     CDS             complement(17772..18008)
                     /gene="lpp"
                     /locus_tag="QIE41_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDS3531943.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4780 a         4397 c         4695 g         4353 t
ORIGIN      
        1 atagttaata ctgtgcgcaa atcatttacc gagatttgcc ctggtgcaga ggcctttttt
       61 accgcaccaa aagttgccgc cgagccaaat acttcaccag ccagacgaga aattacgcca
      121 gtttttgcca ttgacatcgt gatgattgga cgatcggcat actgctcctg catctccagg
      181 gtcgcggcaa gcaacgtcag cacatcgctg gtactttgcg gcatcagcgc aatcttagga
      241 atatcggcgt cgaaggactg cattttgcgc agacgggcaa tgatttcttc ggcttccggc
      301 gttttatgga agtcatggtt ggacatgaca actttcacat catgcgcgtg ggcgtaggcg
      361 acggtttctt taacctgatc atcgccggta aataactcca gatcgatcat atcaaccagg
      421 ccgctgtcga tggctgcacg attgagtgca atataagcct cggtggaaat cgcctgctcg
      481 ccgccttctt tggcactgcg gaaggtaaac agcagcggtt tttctggcat ggtttcacgg
      541 agaatttttg ccgccgccat gacagaatcc acattggaga ggtcggcata gtggtcaaca
      601 cgccattcca gaatatcaaa gtccgcttca cgataggcga gagcttcaga tttcacgctg
      661 gcgatatctt tcgccatcag cgagacgatg attttaggtg cgcccgtacc aatgacgaga
      721 tcttttacag ttacggtttt cattttttac cctttctgca cgcggtcagc ctgtcaggca
      781 ccgaacccca tgacctgttt aacatattcc agagggaaat ctttgccagt ccataacgtg
      841 aactgttcag ccccttgcca caacaacatg ccgtatccat caatcgtttt gcaaccagct
      901 tgttgcgcct gctgcaataa cttcgtcata tgcgggttat acacgcattc agtgaccaga
      961 agtcccggat gtaacagact gatatcatta accaatgatt cattctcaag gggtttcata
     1021 cccacttttg tgccattggt taaaatgtcg gcggaagcca gggcttcagc aaaggcttgc
     1081 tgatcggcga gatcggtaac cgtgacgaca caatcggtgt tttcgttaac ccgctgcgcg
     1141 aaggcgaggg ctttatcgaa gaactcatcc cgacggttaa agagtttaat ttcttttaaa
     1201 ccttcaattg ccccctgcgc gccaatcgcc gttgaggcac ctccggcccc taacagcacc
     1261 atcgttttgc ctttgatatc aaaaccgctc tctttaatgg cgcgaatatg gcccgtgccg
     1321 tcggtgttat agccacgcag atagccatca tcattaacga tggtgttgat ggcacccacc
     1381 agtttggccg ctggcgttaa ttcatcaaca tattcacacg ccagttgttt gttcggcatt
     1441 gatacaccag ttccgcgcat tttgagggct tttaatcctt caattgctcc gggaaaacta
     1501 tcgttatcca cttcaaaagc catataggta aatggcaatc ccgctttttc taaggcttta
     1561 ttctgcattt caggcgataa actgtggcgg atgggatatg ccatcagccc aatcaattcg
     1621 tattttgcgg taacatccat aattaactcc tttaacctct atgcttaatt gtgtttaacc
     1681 gggtgctaaa tttacgctcg ccaaaccgca catcattttc aggaataatg aaaacgcggt
     1741 aatagcggat aaagacaata attgcggtaa tcagggccag cagcgcgaaa gtaaaatcga
     1801 gagcaatgat atattgcagg ccgatgttcg acagataacc ggtaatcagc ggaataacaa
     1861 aattagccag tccacccatc atcatatata tactggtgac tttggctttg ctttttggga
     1921 aaaactctga catgaccgaa acgccgagct gtaaaatgcc gccagctgct gagaaaccga
     1981 taacaaaggc tccggcatta cacaccaatg gagaagggta caggtagata atggcagctg
     2041 ttattgttgc cagtgcagag ttaaatacat tagcccagat gggccggacc atttttttca
     2101 gcagtgcggc aaaaataaag acacagacca acgagcccat actgtaataa gagatggttt
     2161 ttaatgcctc agcttctgac ataccagcaa aagccatcgc atatttgggc atccacacca
     2221 caatcacata aaaggtcgag aatgcggcta caccgaacag tactgatgaa acaccttcca
     2281 gccagactaa cggtttgctg ttcatttgcg gtaattcatt agctacgctg gcgtccacca
     2341 actggctggg gaatttgctt ttcaacagca tcagtgtgat cagtacaaac agaatacccg
     2401 gaataatcag cccatagccg taccagatgt tattgagcaa catatagctc accagcattg
     2461 ggtagaacat ttgcccgaat gacaccatcg ctttaaccag tatgaccgcc gaaccagagg
     2521 ctttcggaaa gcattccatg agcgcggggt agccacccgt atccagcgct gagttagcga
     2581 tacctacgca cactgccaga cagtaggcga gagttaaatt cgggcaagcg ggaataccaa
     2641 agaagaatag cagatacatt attactgcca ttaatatcac cgcccgacga ccaaacttat
     2701 cggagatcac accgaagaat aaaatactga tcaatcgccc caaaccgata ccggaaatta
     2761 aataggcaat gcccgcgttg tcagtggaaa acttttctgc cagagatgac atattttggg
     2821 caagcgtaat aacactaatg ccgtgcagga aatagctgaa gtaaatacaa agaacagcca
     2881 ggataaatgg cgtgctgaaa gccttatttt gagacatttt cacagctcct tgctgaaaga
     2941 taatttcgtt accgtacgta ttgttctccg acggttcaca taattactat actgaagatg
     3001 attttccctg ggtacatggc ataaagttta tgtttccagg aatattgcat gtcatagttt
     3061 gttttttatc gcgattttcg ttagttggcg cagaaagagt tatttatccg taagatcttg
     3121 catgacctga aaagaatatt gcattaccca cccggagcga cattttcctt tagcgagtga
     3181 tgccgcgttt ctttgcggct gcgtaagccg ataaacagtg ccagtaaaaa gccgatggca
     3241 gcgatggcgg tatcgaacca cataatatcg gcaatacttc tttgggacag atgagccgta
     3301 atcaacggaa tagtaaaggt cgcaatactg cccgcactgt aatagatccc tgtagcttta
     3361 cctttagcgt aggggaaacg ttcagccatt aatgtcaggc caatttgcac aacacctcca
     3421 gcagaggtaa aaccaatgac aaaagcaaat attatcacca cataaaatgt gggatgcagg
     3481 cagacggtaa acagagcagt aaatgagata aaggtgtaca gcatgagtaa tgttgttgct
     3541 cgaacggtat tacgaatgag tggagcggta ataaatacac aaagcagcga acccacggtg
     3601 tagatactga gtagtttgat cgacatagtg tatgacatgc ctgcaacaaa ttgtccgtac
     3661 tgtgccagcc actggctaac cagataaaac gttgccattg agatatagcc atataaggta
     3721 taactggcta aatcgattat tgagcagcga tgttccgtag agctggtggt tttctttatg
     3781 acaggtaagc gacgacccgg atggggtggg aacgtacaac ggtataaaaa cagagcgtta
     3841 ataaacataa tgcctgcggc aatcataaag gaccaaccga accacagttc agcccacact
     3901 aacaggctaa tgattagcgg taataaaaat tgcccgctgg aaacaaatgc tttaattaaa
     3961 atattggctg tacccggtga gcgtggaaaa gcttccatca aactgggata ggtgcctgca
     4021 tcgagaaaac tgtttgccat tcccgccaga aagccaaaaa cataagcgat aatgatgtta
     4081 ttggtgtgca ggatgccaaa aaagaaggcc atatagcaac acatcccgag catgataaaa
     4141 gggcggcgac caaagcgatc ggataataat cctgcaaaaa gcaagacact taatcgacca
     4201 atacccagcg atgagataac tattgagacg cctgcggcat tagtctgcca aagcgtctcc
     4261 agcgaggcca tattcaggct catcaaaatg acgcccatac catgcaccag gtaattaaaa
     4321 tacaacccca gtgcggtagg gtaataggga tttttcatag gttctactta gcaaaggtgc
     4381 tttaaaaagt gaagcgatgt tgtgtcagca tccgttcatt attgttgtaa caacgacaat
     4441 tcctttcttt ggaaaattaa caaaggcgct ccagatcgtg tgcatataat tcagcggcta
     4501 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     4561 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     4621 tggcatgaag atgttttttt ctttgctgac gcatagccaa cagttgctcg actacacgat
     4681 caggaatggc gtatttgcca ttttcccatt gacgccagct ttcactgtca ccggtctggg
     4741 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     4801 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     4861 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagcgtg
     4921 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     4981 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5041 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5101 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctaccagctg aatggcggta
     5161 agcaggaaaa tatggggaag agcaagcaaa tatcaattag cggtggttaa caatcaggcg
     5221 gcagagccgc ctgatgagaa attacttatt cggtttctcg gtttcgccaa gttcttcgag
     5281 aatttcttcc ggttggtccg tcggcggtgg gacctcttcc acccacgcgg caaacagacg
     5341 ccaggaaacg gctaaaagaa ccggaccaat aaatagaccg atcatcccga aagcaatcaa
     5401 accaccaata acaccagaga ggatcaggat cagcggtaaa tcggcaccca tgcgaattaa
     5461 cattgggcgg atgacgttat ccagcgtgcc aaccacaccg ctccacacta acaataccgt
     5521 tccccaggtg gtatcaccgg tccagtagag ccagataatc gccggaatca gtaccggcaa
     5581 cggtccaagc tggacaaggc aggagaggat cattaacacc gtgagcaacg ttgcataagg
     5641 tacgccggat acggcgaggc cgataccgcc aagcactgcc tgtactaacg ctgtcaccac
     5701 aacacccagc gccaccgcgc ggatagcttg tgccgctagc agcacggcgg catctccacg
     5761 aacgcctgcc agacgggttg caaaatggcg aatgccttgc cccacttgtt caccgcgcca
     5821 gtacagcagg gcgctgaaga gaagcatcag cgcacaatgc accataaagc gcccgatatg
     5881 cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata taaggacgga cttttgccat
     5941 gatcgccgtc ccccccatat ccagcaagtt gtgccagcct gcatacagct tcgcgccaat
     6001 caccggaatg gtattaagcc acgccagatc gggtaacgtc atgtcaccgc tggaaatggc
     6061 tttaattagc gggccgctgc cgtcgacgat actgttaacc agcaaagcaa tagggatgat
     6121 aaacaccatc actaataaca gcgtcatcac cagaacggcg agagagcggc ggccaaacat
     6181 gatcttttgc aaacgtaaca ataccggcca ggtggcgata accaccgtac cggcccatgc
     6241 aaagccgaga ataaagggtt gaacaatcca cagacatgcc acaatcatga tggctaaaaa
     6301 cagcaccgaa agcagaattt gtgcgacatc cctgggctga cgaacattta ccataacaac
     6361 ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg aacacgcgaa actcacctgc
     6421 ataatgatga gcaatttcag cggtttttaa caggccgatt ctgcccttaa ttctgatggg
     6481 cgtgttagaa aaaaatgtgg tacaacaact caggcaacac gcaaacgatt tactcgtcgt
     6541 atttcaagtt gcatgtgctg cggctacgtt cgttcacccc agtcacttac tattgtaagc
     6601 tcctggagtt cactcgcttg ccgcctttct gcaacacgaa atccatagag taaacaaaca
     6661 tcacctttca gtgcgctatt gcagcagcct gaaagaatag tgtatcgacc tacatcacag
     6721 accgcaggaa agggtcaata taatgattcc acagatttcc caggcacccg gcgtcgttca
     6781 actggtgctt aattttttgc aagagctgga gcaacagggg tttaccggcg atacggcgac
     6841 aagttatgcc gatcgtctga caatgtcgac cgacaacagt atttaccaac ttctccccga
     6901 tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg atcgcccgtc ttgccgcgca
     6961 ggaacgctat tcatcgctga tctttacccc ccgcggcggc ggcaccggca ctaacggtca
     7021 ggcgctcaac caggggatta ttgttgatat gtcccgccat atgaaccgca tcatcgaaat
     7081 taaccctgaa gagggctggg tgcgcgttga ggccggggtg ataaaagatc aactcaatca
     7141 gtacctgaaa ccgttcggct acttttttgc gccggaactt tcgaccagca accgggcaac
     7201 gctcggtggg atgatcaata ccgatgcatc cggtcaggga tcgctggtct atggcaaaac
     7261 gtcagatcac gtacttggcg tacgcgcggt gttgttgggg ggcgatattc tcgatacgca
     7321 acctttaccc gtcgagctgg cggaaacgct gggtaaatcc aataccacaa tcgggcgaat
     7381 ttataacacg gtttatcaac gttgccgtca gcaacgccag ttaatcatcg acaacttccc
     7441 caaacttaac cgctttctta ccggttacga tctgcgtcat gtctttaacg acgagatgac
     7501 cgagtttgac ctgacgcgca ttctgacggg ttcagaaggg acgctggcct ttattaccga
     7561 agcgcggctg gatattacgc ccttgcctaa agtgcgccgt ctggtgaacg tcaaatatga
     7621 ctcttttgac tccgcgctgc gtaacgcgcc gtttatggtt gaggcgcggg cgctttcggt
     7681 agagacggtg gactcaaaag tgctgaatct ggcgcgggaa gatattgtct ggcattccgt
     7741 cagcgagttg attaccgatg tgcctgacaa agaaatgctc gggctgaaca ttgtggaatt
     7801 tgctggtgat gatgagacgc tgattgatga gcgagtaaat gcactctgtg cgcggctgga
     7861 tgaacttatc gccagtcagc aagcaggtgt gatcggctgg caggtatgca gtgagctggc
     7921 aggtgttgag cgtatttatg cgatgcgcaa aaaagctgtc ggcctgctcg gtaatgccaa
     7981 aggagcagcc aagccaattc cgtttgctga ggatacctgc gtaccgccgg aacatctggc
     8041 agattatatt gctgaatttc gcgcgctgct cgacagccac ggcttaagct acggtatgtt
     8101 tggtcacgtc gatgcaggtg tcctgcacgt ccgtccggca ctggatatgt gcgatccgca
     8161 acaagagatt ttgatgaagc aaatctctga tgatgtagtg gcgcttaccg cgaaatacgg
     8221 tggtttgttg tggggcgagc acggcaaagg ttttcgcgcc gaatacagcc cggcgttttt
     8281 cggtgaggaa ctttttgcag aactgcgtaa agtgaaagcg gcatttgacc cgcataaccg
     8341 actcaaccca gggaagattt gcccgccaga aggtctcgat gcaccgatga tgaaagtgga
     8401 cgcggtgaag cgtggcacat atgatcgaca gatccccatt gcggtacgcc agcagtggcg
     8461 tggtgcgatg gagtgtaacg gcaacggttt atgcttcaac tttgatgccc gcagtccgat
     8521 gtgcccgtcg atgaagatta cccagaaccg gattcattca ccgaaagggc gcgcaacgct
     8581 ggtgcgtgaa tggctgcgtt tgttggcaga tcgcggtgtt gacccagtca aactggaaca
     8641 agagctgcct gaatcaggcg tcagtttgcg gacgttaatt gcccgcacgc gcaatagctg
     8701 gcatgcgaat aaaggcgaat atgacttctc ccacgaagtc aaagaggcga tgtcgggctg
     8761 tctggcctgt aaagcttgtt cgacccagtg cccaatcaaa attgacgtgc ctgagtttcg
     8821 ttctcgcttt ctgcaactct atcacacccg ttatttgcgc ccgctgcgcg accacctcgt
     8881 cgccacggtc gagagctacg caccgctgat ggcacgcgcg ccgaaaacct ttaacttctt
     8941 cattaaccag ccgctggtgc gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc
     9001 gctgctgtcg gtcccctcgc tacaacaaca aatggtgggg catcgctcgg caaacatgac
     9061 gctggaacag cttgaagctc tcagtgcaga gcagaaagcg cgcacggtgc tggtggtgca
     9121 ggaccccttt accagctatt acgatgcgca agtggtggcg gattttgtcc gtctggtcga
     9181 aaaattaggt ttccagccgg tgttactgcc attttcgcca aatggcaaag cccagcatat
     9241 caaaggcttt cttaatcgtt ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg
     9301 tgtggcgaag ctggggatgc caatggtggg agtcgatccg gcgctggtac tttgttatcg
     9361 cgatgaatat aaactggcgc tgggcgagga acgtggcgag tttaacgtct tactggcgaa
     9421 tgaatggctg gcaagcgcac ttgactcaca gccagtggct acagtcagcg gtgaatcatg
     9481 gtatttcttt ggtcactgta ccgaagttac cgctttgccg ggcgcgccag cacaatgggc
     9541 cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc agcgtgggtt gctgcggcat
     9601 ggcagggact tacggacatg aagcgaaaaa ccataaaaat tcgctcggga tctatgagtt
     9661 atcctggcat caggcgatgc agcgactgcc gcgtaatcgc tgtctggcga ccggatattc
     9721 ctgccgtagc caggtaaaac gggtcgaagg cacgggggta cgccatcctg tgcaggcttt
     9781 actggagatt attaaatgat atggaaacga aaaatcaccc tggaagcact gaatgccatg
     9841 ggtgaaggaa acatggtggg gttgctggat attcgctttg aacatattgg tgatgacacc
     9901 cttgaagcga caatgccagt agactcgcgg acaaagcagc ctttcgggtt gctgcatgga
     9961 ggggcatccg tggtactggc cgaaagtatc ggttccgttg ccggttattt atgtaccgaa
    10021 ggtgagcaaa aggtggttgg tctggaaatc aatgctaacc acgtccgctc ggcacgagaa
    10081 gggcgggtgc gcggcgtatg caaaccgttg catctcggtt cgcgtcacca ggtctggcag
    10141 attgaaatct tcgatgagaa agggcgtttg tgctgttcgt cacgattgac gaccgccatt
    10201 ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt ttggtcatta tctgcacagt
    10261 aaacggtggt atactgtgca ggtcttgtct aaaagcgagg atgctatgtc tactcaactt
    10321 gatcccaccc agctggccat tgaattttta cgccgtgacc aaagcaatct ttcacctgcc
    10381 caatatctca aacgtctgaa acaactagaa cttgaatttg ctgacctgct tactctctct
    10441 tctgctgaat taaaagaaga aatctacttt gcctggcgtc tgggcgtgca ttgatcctga
    10501 cttgatgccg caatttcgcc aaatcgacct ggattaacct ccgtccaggt tcgatggcct
    10561 tcttaatact gcttatctac ccgcctgata ttgttcaaaa ataatactaa tcgcagtttg
    10621 tgttaaaacg gcgggttagc tttatgagat tattcttatc gccccttcaa gagctaagcc
    10681 actgagagtg ccggagataa gcgccggatg gggtagaaac ccttaagcct gtgtcgcaca
    10741 gacttaaggg ttttcttatt tctgaacgct gcactttttt acctatctct taaatagtca
    10801 ttttcaatac aactttttct ttttcatcac agccttaatg ctccttagat aataatcatt
    10861 atctaacaat gagataccta attcttagcg tgcctgttaa cccacacatc agggtctatg
    10921 cttattaaaa gcaggaaaaa agcatgtttt acggtaaagc ccctgcgttt gctgggttga
    10981 actgataatc attatcacta acatgctgtt atacgctgaa agcgatgaag tgaggtaaat
    11041 cgatggacat gcattcagga acctttaatc cacaggattt cgcctggcag ggcttaacgc
    11101 tgacacccgc agccgctgtc cacatccgcg agctggtggc aaagcagccg ggtatggtcg
    11161 gcgtgcgctt aggcgtgaag caaacgggct gcgcgggctt tggctatgtg ctcgacagtg
    11221 ttagcgagcc ggacaaagac gatctgctgt ttgaacacga cggcgcgaag ctgtttgtcc
    11281 cgctgcaagc gatgccgttt attgatggca cggaagtcga ttttgttcgt gaaggactta
    11341 atcagatatt caaatttcac aaccctaaag cccagaatga atgtggctgt ggcgaaagct
    11401 ttggggtata ggcggtacta tgtctcgtaa tactgaagca actgacgatg tcaaaacctg
    11461 gaccggcggc ccgctgaatt ataaagaagg attattcacc cagttagcca ccgatgagct
    11521 ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg gcgaagcgta atgagccgga
    11581 gtggatgctg gagtttcgtc tcaacgccta tcgcgcatgg ctggagatgg aagaaccgca
    11641 ctggttgaaa gcgcactacg acaagctgaa ttatcaggat tacagctact actcagcacc
    11701 atcgtgcggt aattgtgacg acacttgtgc gtctgaaccc ggcgtggtgc agcaaactgg
    11761 cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt gagcagttgg gcgttcccgt
    11821 gcgggaaggc aaagaggtgg cggtggacgc cattttcgac tctgtttcgg ttgccaccac
    11881 ttatcgcgaa aaactggcgg agcagggaat tattttctgt tcctttggcg aggcgatcca
    11941 cgatcacccg gaactggtgc gtaaatatct cggcaccgtg gtgccgggga atgacaactt
    12001 ctttgccgca cttaatgcgg cggtagcctc tgacggtacg tttatttatg tgcctaaagg
    12061 cgtgcgctgc ccgatggaac tttccaccta ttttcgtatt aacgcggaaa aaaccgggca
    12121 gtttgagcgc accattctgg tggccgacga agacagctac gttagctaca ttgaaggctg
    12181 ttccgctccg gtgcgtgaca gctatcagtt acacgcggcg gtggtggaag tcatcatcca
    12241 taaaaacgcc gaggtgaaat attccacggt acaaaactgg ttccctggcg ataacaacac
    12301 cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc gaaggcgaaa acagcaaaat
    12361 gtcatggacg caatcagaaa ccgggtcagc gattacgtgg aaatatccca gctgtatttt
    12421 gcgcggcgat aactccattg gtgagtttta ctcagtggcg ctgaccagcg gtcatcagca
    12481 agcggatacc ggcaccaaga tgatccacat cggtaaaaac actaaatcga ccattatctc
    12541 gaaagggatc tctgccggac atagtcagaa cagttatcgc ggcttagtga aaatcatgcc
    12601 gacggcaacc aatgcgcgca atttcactca gtgcgactca atgctgattg gcgctaattg
    12661 tggggcgcat accttcccgt atgtcgaatg tcgcaataac agcgcacaac tggagcacga
    12721 ggcaacaaca tcacgtattg gtgaagatca actgttttac tgcctgcaac gcgggatcag
    12781 cgaagaagac gccatctcga tgattgttaa cggtttctgc aaagacgtgt tctcagaact
    12841 gccgctggaa tttgccgttg aagcacaaaa actcctcgcc atcagtcttg aacacagcgt
    12901 cggataagga ataaacatgt taagtattaa agatttacac gtcagcgtgg aagataaagc
    12961 tatcctgcgt ggattaagcc tcgacgttcg tcccggcgaa gttcacgcca ttatggggcc
    13021 aaacggttct ggcaaaagta ccttatcggc aacgcttgcc gggcgagaag attatgaggt
    13081 gacgggcggc acggttgagt tcaaaggcaa agatttgctt gcgctgtcgc cggaagatcg
    13141 tgcaggtgaa ggcatcttta tggccttcca gtatccggtg gaaattcccg gcgtcagtaa
    13201 ccagtttttc ctgcaaacgg cacttaatgc ggtgcgcagc tatcgcggcc aggaaacgct
    13261 cgaccgcttt gattttcagg atttgatgga agagaaaatc gctctcctga agatgccgga
    13321 ggatttatta acccgttcgg taaacgttgg tttttccggc ggcgagaaaa agcgcaacga
    13381 tattttgcaa atggcggtgc tggaaccgga gttatgcatt cttgatgagt cggactccgg
    13441 gctggatatt gacgcattaa aagtggttgc cgatggcgtg aactcgctgc gtgacggcaa
    13501 gcgctcattc atcattgtta cgcactacca acgcattctc gactacatca agcctgatta
    13561 cgtccatgta ctatatcagg gacgaattgt gaaatccggc gatttcacgt tggtcaaaca
    13621 actggaggag cagggttatg gctggcttac cgaacagcag taacgcgctg caacagtggc
    13681 atcacttgtt tgaagctgaa gggacaaaac gctcaccgca agcacagcag catttacaac
    13741 aattgctgcg taccggactg ccgacacgta aacatgaaaa ctggaaatat acgccgctgg
    13801 aagggctgac caatagccag tttgtcagca ttgcgggaga gatatcccca cagcagcgtg
    13861 atgccttagc gttaacgtca gacgccgtgc ggctggtgtt tgttgatgga cgttacgtgc
    13921 ccgcgctgag cgatgcaact gaaggcagcg gatatgaagt gagcattaac gacgaccgtc
    13981 aggggttacc cgacgctatt caggcggaag tgtttctgca tttgacggaa agccttgcgc
    14041 atagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc ggtaaagcca ttgctgttaa
    14101 tgcatatcac ccagggcgtg gcaggtgaag aggtgaacac tgcccattac cgacatcatc
    14161 tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca ttttgtcagc cttaatgatg
    14221 ctcgtcattt taccggggca cggttcacta tcaatgtcgc agcgaacgcc cacttgcagc
    14281 atatcaagct ggcgtttgaa aacccgctca gtcaccactt tgcccataac gatttgttgc
    14341 tggctgacga tgccaccgca tttagccaca gtttcctgct gggtggcgca gtgttacgac
    14401 acaacaccag tacgcaactc aatggcgaaa acagcacgct gcggatcaat agcctggcga
    14461 tgccggtgaa aaacgaggtg tgtgatactc gtacctggct ggaacacaat aaaggttttt
    14521 gtaacagccg acagttgcac aaaactatcg tcagcgacaa aggccgcgcg gtatttaacg
    14581 gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg tcagatgacc aacaataatc
    14641 tgctgatggg caaactggcg gaagtggata cgaaaccgca gctggaaatc tatgcagatg
    14701 atgtgaaatg cagccacggc gcgacggtgg ggcgcattga tgacgaacag atgttttatc
    14761 tgcgctcgcg cgggatcaat cagcaagatg cccagcagat gatcatctac gcctttgctg
    14821 ctgaactgac ggaagcactg cgtgatgagg ggcttaaaca gcaggtgctg gcccgaatcg
    14881 gtcaacggct gccaggaggt gcaagatgac tttttccgtc gacaaagtgc gggccgactt
    14941 tccggtgctt tcgcgtgagg taaacggttt gccgctggct tatctcgaca gcgccgccag
    15001 tgcgcagaaa ccgagccagg tgattgatgc cgaggctgag ttttatcgtc atggctacgc
    15061 ggcggtgcat cgcggtattc ataccttaag cgcccaggcg accgagaaaa tggagaacgt
    15121 gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg gaagagctgg tgttcgtccg
    15181 cggcacgacg gaagggatca atctggtcgc caatagctgg ggtaacagca atgtgcgggc
    15241 gggcgataac atcatcatca gtcagatgga gcaccacgct aacattgttc cctggcagat
    15301 gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg ctcaatcccg acggtacgtt
    15361 gcaactggag acgctaccta cgctgtttga tgagaaaact cgcctgctgg caattactca
    15421 tgtctccaac gtgctgggaa cggaaaatcc gctggcggaa atgatcacgc ttgcgcatca
    15481 gcatggcaca aaagtactgg tggatggcgc tcaggcggtg atgcatcatc cggtggacgt
    15541 tcaggcgctg gattgcgatt tttacgtgtt ctccgggcat aaactgtatg gccccactgg
    15601 gattggcatt ctttatgtca aagaagcctt gttgcaggag atgccgccgt gggaaggggg
    15661 cggttctatg atcgccaccg tcagcctgag tgaaggcact acctggacca aagcaccttg
    15721 gcggtttgaa gccggtacac ccaataccgg gggcatcatt ggtcttggcg cggcgctgga
    15781 gtatgtgtcg gcgctggggc ttaataatat agccgagtat gaacagaatc tgatgcacta
    15841 cgcgctatca cagctggaat ctgtaccgga tctcactctc tatggtcccc agaacagact
    15901 tggcgttatt gcttttaatc ttggtaaaca ccacgcctat gatgttggca gttttctcga
    15961 taattacggc attgctgtgc gtaccggaca tcactgcgct atgccattaa tggcctatta
    16021 caacgttcct gccatgtgtc gggcgtcgct ggctatgtat aacacccatg aagaagtgga
    16081 tcgtctggtg accggcctgc aacgtattca ccgtctgctg ggataacagg gaggcactat
    16141 ggctttattg ccggataaag aaaaattgct gcgtaatttt ttacgctgcg ccaactggga
    16201 agagaaatat ctctacatta ttgagctggg ccagcgtctg ccagaattac gcgacgaaga
    16261 cagaagctca caaaatagca ttcagggctg ccagagtcag gtgtggattg tcatgcgcca
    16321 gaatgcgcag ggaattattg aattgcaggg cgacagcgat gcggcgattg tgaaagggct
    16381 tattgcggtc gtctttattc tctacgatca gatgacgccg caggatattg tcaatttcga
    16441 tgtgcgtccg tggtttgaaa aaatggcgct cactcaacat ctcaccccat ctcgttcaca
    16501 aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc gctgcactta gctaaactaa
    16561 aggaacagct ttcatccggt tggctcacat tgccggatgg tcaatgttta cactgacctg
    16621 cacaggagtg ttttggtttc aggtgaacat aagcgccatc cgacgtttca gcgtgagtct
    16681 ccggcaaata caggaggttt acaatgaaac gcgcgtcttt gcttacactc acgcttatcg
    16741 gcgcttttag cgccatccag gctgcctggg cggttgatta tccgctacct ccaaccggaa
    16801 gccgactggt tgggcaaaat caaacgtata cggtgcaaga aggggataaa aaccttcagg
    16861 ccatcgcccg acgttttgat actgcggcaa tgttgatcct tgaagccaat aacactatcg
    16921 ccccggtgcc aaaacctggt acgacgataa ctattccttc gcaactgtta ttacctgatg
    16981 cgccgcgtca ggggattatc gttaacctgg cagagctgcg cctttattat tatccgccgg
    17041 gagaaaatat cgtgcaggtc tatccgatag gtattggttt gcaggggctg gaaacgccag
    17101 tgatggaaac gcgtgtgggg cagaaaatcc ctaacccaac ttggacgcct acggcgggca
    17161 ttcgtcagcg ttcgctggag cgtggcatta aattaccgcc agtcgttcct gccgggccaa
    17221 ataacccgct aggacgttac gcactgcgcc tcgcgcatgg taatggcgaa tacctcattc
    17281 atggcaccag tgcgccggac agcgtcggtt tgcgcgtcag ttcgggctgt attcgcatga
    17341 atgcgccgga tattaaagcc ttattctcca gcgtgcggac gggaacgccg gtgaaagtga
    17401 tcaacgaacc ggtgaaatat tccgtggaac cgaacgggat gcgttatgtt gaagtacatc
    17461 gaccactatc agcagaagaa cagcagaacg ttcagacaat gccatacaca ctgccagcag
    17521 gctttacgca atttaaagac aataagggtg tagatcagaa attagtcgat aaagcgttgt
    17581 atcgtcgggc agggtatccg gttgcggtga gcagtggagc aactcccgca gccagcaatg
    17641 cgccttcagt agagtcagcg cagaatggtg aaccagagca agggaatatg ttacgcgcga
    17701 cgcagtagcg gtaaatggca ggcaaaaaaa atggcgcaca atgtgcgcca tttttcactt
    17761 cacaggtact attacttgcg gtatttagta gccatgttgt ccagacgctg gttagcacga
    17821 gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg cgttcacgtc gttgctcagc
    17881 tggtcaactt tagcgttcag agtctgaacg tcagaagaca gctgatcgat tttagcgttg
    17941 ctggagcaac ctgccagcag agtagaaccc aggattaccg cgcccagtac cagtttagta
    18001 gctttcatta ttaataccct ctagattgag ttaatctcca tgtagcgtta caagtattac
    18061 acaaactttt ttatgttgag aatatttttt tgatgggaag gcacttattt ttgatcgttc
    18121 gctcaaagaa gcatcgaacg ctgggtttta gataaaaaaa ttgagagaaa acaggatgct
    18181 tccatcggat tcatcttaga taaagtgaga ttatatagtg aaatc
//
LOCUS       DAOTRK010000094        18150 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.094, whole genome shotgun sequence.
ACCESSION   DAOTRK010000094 DAOTRK010000000
VERSION     DAOTRK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.094"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            130..669
                     /locus_tag="QIE41_003082"
     CDS             130..669
                     /locus_tag="QIE41_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3531944.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQNNGQVSGVQTDVPIELKDCDITVTKNATFTFNGTADT
                     TQITAFANQASTDAATNVALQMYMNDGTTTITPDTETGNILLQNGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            752..1453
                     /locus_tag="QIE41_003083"
     CDS             752..1453
                     /locus_tag="QIE41_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDS3531945.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGP
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLAEAPAKLR
                     CTSLADMVMVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFPLPKGAQNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1478..4078
                     /locus_tag="QIE41_003084"
     CDS             1478..4078
                     /locus_tag="QIE41_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDS3531946.1"
                     /translation="MPRTHRQHSLLTSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFERGQKITPGVYRVDIVLNQTMVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACAPLAEIIPDASVTFDVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSSDSDSTDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSINSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNDQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMLGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGDYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDSGSQQQLNY
                     ALSGSLVAHSRGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIVLDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINA
                     AITRMDAICR"
     gene            4069..5139
                     /locus_tag="QIE41_003085"
     CDS             4069..5139
                     /locus_tag="QIE41_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3531947.1"
                     /translation="MQIIYGEKCVSLLRLFFAAILMLWCAQTMAYTGQCHTAQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDSWYKINDYLAARVLLQVKGNSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFIGSVVIPPMTLARLFECYNIPAGDSCTSSGDPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVKVNLSL
                     TATSDPSYPQAIKTSRPGVGIVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5151..5693
                     /locus_tag="QIE41_003086"
     CDS             5151..5693
                     /locus_tag="QIE41_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3531948.1"
                     /translation="MKKKTIFQYVILFFSFLNTHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5701..6216
                     /locus_tag="QIE41_003087"
     CDS             5701..6216
                     /locus_tag="QIE41_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDS3531949.1"
                     /translation="MLKRIIWILCLLGLTGSCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGMQSEMLSI
                     ESGTDAASGLAIAILDDVKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6182..6919
                     /locus_tag="QIE41_003088"
     CDS             6182..6919
                     /locus_tag="QIE41_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDS3531950.1"
                     /translation="MPARHLYFIMTNTWNRLTLSIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISISLTNTSQDSWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DLLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVSWRTINDYGGLSAKKEQNLL"
     gene            7030..8040
                     /gene="pyrD"
                     /locus_tag="QIE41_003089"
     CDS             7030..8040
                     /gene="pyrD"
                     /locus_tag="QIE41_003089"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDS3531951.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8214..8756
                     /gene="zapC"
                     /locus_tag="QIE41_003090"
     CDS             8214..8756
                     /gene="zapC"
                     /locus_tag="QIE41_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDS3531952.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8753..9862)
                     /gene="ycbX"
                     /locus_tag="QIE41_003091"
     CDS             complement(8753..9862)
                     /gene="ycbX"
                     /locus_tag="QIE41_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDS3531953.1"
                     /translation="MATLTRLFVHPVKSMRGIGLTHALADISGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            10106..12214
                     /gene="rlmKL"
                     /locus_tag="QIE41_003092"
     CDS             10106..12214
                     /gene="rlmKL"
                     /locus_tag="QIE41_003092"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDS3531954.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12226..14133
                     /locus_tag="QIE41_003093"
     CDS             12226..14133
                     /locus_tag="QIE41_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3531955.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14263..15516
                     /gene="pqiA"
                     /locus_tag="QIE41_003094"
     CDS             14263..15516
                     /gene="pqiA"
                     /locus_tag="QIE41_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDS3531956.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15521..17161
                     /gene="pqiB"
                     /locus_tag="QIE41_003095"
     CDS             15521..17161
                     /gene="pqiB"
                     /locus_tag="QIE41_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDS3531957.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17158..17721
                     /gene="pqiC"
                     /locus_tag="QIE41_003096"
     CDS             17158..17721
                     /gene="pqiC"
                     /locus_tag="QIE41_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDS3531958.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17977..18144
                     /gene="rmf"
                     /locus_tag="QIE41_003097"
     CDS             17977..18144
                     /gene="rmf"
                     /locus_tag="QIE41_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDS3531959.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
BASE COUNT         4677 a         4167 c         4789 g         4517 t
ORIGIN      
        1 ataaattaaa gatttgtcac ttattggatt tagtatgctt gatttaaatg gcggacaatt
       61 attgttatta ttaagtctaa tgtcatttaa aggcattatc atatttacga agagatcagg
      121 atgataacga tgaaaaaaag tgtattgacg gcgtttataa ctgtggtatg tgcaacgtcc
      181 agtgttatgg cagctgatga taatgctatc acggatggct cagtaacatt taatggtaaa
      241 gtcattgccc cagcgtgtac gctggtagct gcgacgaaag attccgtggt gactttgcca
      301 gatgttagtg ctacgaagtt gcaaaataat ggccaggttt ctggggtgca aactgatgtg
      361 ccaattgaat taaaagattg tgatattacc gtaacaaaaa atgcaacgtt cacctttaat
      421 ggcactgcgg atactacgca gattacagcg tttgctaatc aggcctctac tgatgctgct
      481 acaaacgtgg ccctgcaaat gtacatgaat gatggcacaa cgaccatcac gccagacaca
      541 gaaaccggga acatcttgtt gcagaatgga gatcagacgt tgacctttaa agttgattat
      601 atcgctacgg gtaaagccac ttcaggtaat gttaatgcgg taacaaactt ccatattaac
      661 tattattaat agaacacatt aattgtttta ttaattattc cccctccagt gttctggagg
      721 ggatatccat attttttaag agtgactatt tatgaaaact tgcataacaa agggaattgt
      781 gaccgtaagt ttaacggcaa tattactcag ctgttcgtcc acatgggcgg caggtaaagg
      841 cggggttgga cttgcagcaa cacgtcttgt ttattcggaa ggtgaagagc aaatttcact
      901 gggtgtacgt aacaccagtc cggatgttcc ttatctgatt cagtcatggg tgatgacacc
      961 tgataataaa aagtcagcgg actttattat tacaccaccg ctatttgtgc tgaatccggc
     1021 aaacgagaat ctgttacgca ttatgtatat tggtccgccg ttggcgaaag acagagaaac
     1081 tcttttcttc accagcgtac gggcagtccc ttcaacaacg aagcgggaag aaggcaatac
     1141 cctgaagatt gccacacaaa gcgtcatcaa acttttctgg cgaccaaaag gtttagcata
     1201 tcccttagcc gaggctccgg cgaaactgcg ttgcacttcg ttagctgaca tggttatggt
     1261 cagtaaccca acaccttatt tcattaccct gacagacctg aaaataggtg gaaaagtagt
     1321 taaaaatcaa atgatctccc cctttgataa ataccaattc cctctgccaa agggtgctca
     1381 aaacagtagc gtaacgtacc gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa
     1441 ctgtaaatcg taagccgtct tcagttaaga gagcgagatg cctagaactc accgacaaca
     1501 cagcctgtta acctctggtg gagtgccatc gtttattggt gggctggtgg tgtttgtgtc
     1561 ggcggcgttc aatgcacaag ctgaaacctg gttcgatccc gcctttttca aagatgatcc
     1621 ctcaatggtg gccgatttgt cccgtttcga aagagggcaa aaaataacgc caggcgttta
     1681 tcgtgtcgat attgttctga atcagacaat ggtagatacg cgcaacgtta attttgttga
     1741 gataacgcca gagaagggga ttgccgcctg cttgactact gaaagcctgg atgcaatggg
     1801 cgtgaatact gatgcgtttc cggcttttaa acaactggac aaacaagcgt gtgcgccatt
     1861 ggcagagatt attccggatg ccagcgtaac ctttgatgtg aataaactcc gtctggaaat
     1921 ttcagtaccg caaattgcta taaaaagtaa cgcccgtggc tatgttcccc ctgaacgctg
     1981 ggatgaaggg atcaacgcac tattactggg atattctttt agcggggcca acagtattca
     2041 tagcagttca gatagtgatt ccacagatag ctattttttg aatttaaaca gtggcgtgaa
     2101 tttaggtccg tggcgattgc gcaataattc cacgtggagt cgtagtagcg gccaaaccgc
     2161 agaatggaag aatctcagca gctatttgca gcgggcggtt attcctctga aaggcgaact
     2221 gaccgtcggt gatgattata ccgcaggcga ttttttcgac agcgtcagct ttcgtggcgt
     2281 acagctggcg tcagatgaca acatgctgcc agacagcctg aaagggtttg cgcctgtggt
     2341 gcgtggtatc gccaaaagca atgcccagat aacgattaag caaaacggct acaccattta
     2401 ccaaacttat gtctcgccag gcgcttttga gattagtgat ctctactcca cgtcgtcgag
     2461 cggtgatttg ctggttgaaa taaaagaagc tgacggcagc atcaatagtt acagcgtgcc
     2521 cttttccagt gtgccattac tccagcgtca ggggcgaatc aaatacgcgg tgacgctggc
     2581 gaaatacaga accaatagta atgatcagca agaaagtaaa tttgctcagg ccacgctgca
     2641 atggggcgga ccgtggggaa cgacctggta tggcggtgga caatatgctg aatattaccg
     2701 tgccgctatg ttggggctgg gttttaacct cggcgatttc ggagcgattt cgttcgacgc
     2761 gacccaggcg aaaagtacac tggcagacca aagtgaacat aaagggcagt catatcgttt
     2821 tctgtatgcc aaaacgctca accaattggg cactaacttt caattgatgg gctatcgcta
     2881 ttcgacgtca ggcttctaca ccctttccga caccatgtat aaacacatgg atggctacga
     2941 atttaatgac ggtgatgatg aagatacgcc gatgtggtcg cgttattaca atttgtttta
     3001 caccaaacgt ggcaaactgc aggtcaatat ctcccagcaa ttaggcgatt acggttcgtt
     3061 ttatttaagt ggtagccagc aaacatactg gcataccgat caacaggatc ggctattaca
     3121 gtttggctac aacacgcaaa ttaaagatct ctcactgggc gtttcctgga actacagtaa
     3181 gtcccgtggt caacctgatg ctgatcaagt atttgcactt aatttttccc tgccgctcaa
     3241 tctgttgctc cccagaagta atgatagcta taccaggaaa aaaaattacg catggatgac
     3301 ctctaatacc agtatcgata acgaagggca cactacacaa aatcttggtt taacggagac
     3361 actgctcgat gacggtaacc tgagctacag cgtgcaacag ggatataaca gcgaggggaa
     3421 aacggcgaat ggtagcgcca gcatggacta caaaggggcg tttgcggatg cccgagtggg
     3481 ctacaactac agcgatagcg gtagccaaca acaacttaac tacgcccttt caggcagttt
     3541 agttgcccat tcacggggca ttaccctggg gcaatcactg ggggaaacca acgttctgat
     3601 tgcagcacca ggcgcggaaa atactcgtgt ggcgaatagc accgggctga aaactgactg
     3661 gcgcggatat accgttgtcc cctatgccac ttcttatcgt gaaaatcgaa tcgtacttga
     3721 tgctgcatcg ttaaaacgta acgtggatct tgaaaatgca gtagtaaatg tagttcccac
     3781 caaaggggcg ttggttctgg cggagttcaa tgcccatgcg ggggcgaggg tattaatgaa
     3841 aacatcaaag cagggtatac cgctgcgatt tggcgcgata gcaacgctgg atggggtaca
     3901 gaccaatagc ggcataattg atgacgatgg ctcgctctat atgtccggtt tgcccgctca
     3961 gggagcgata actgtacgct ggggtgaagc tcccgatcaa atttgtcata tcagttacca
     4021 gcttaccgaa caacaaatta acgctgcgat tacgcgaatg gatgccatat gcagataatc
     4081 tatggagaaa aatgcgtgtc attactacga ctattttttg ccgccatctt aatgctatgg
     4141 tgcgctcaaa ctatggctta taccgggcag tgccataccg ctcaggggaa tccgtatatt
     4201 ggcgtcaatt ttggcgttaa aaccctggat gaagaagaaa atacggccgg agtagttaaa
     4261 gacaaatttt atcagtggaa cgaatcgaat gattactatg tttcttgtga ttgcgataaa
     4321 gacaatgtca ggagtggcag atgggcattc gctgcggatt caccgttagt ctatttaggc
     4381 gacagctggt acaaaattaa tgactatctt gccgccaggg ttttattaca ggttaaaggt
     4441 aattctccta cggcggttcc attcgaaaac gtgggcaccg ggggggatac ccgatggcat
     4501 atttgcgatc ccggcggtca acgtttaggc ggccaggggg ctagcggtaa tagcggtagc
     4561 ttttccctga aaatattgca gccgttcatt ggctcggtcg tcattcctcc tatgacgctg
     4621 gcacgattgt ttgaatgcta caacataccc gcaggggatt cctgtacgtc gtcaggcgac
     4681 ccggttttag tgtattacct gtctggcacg atcaattcac ttggctcatg ttccgtcaat
     4741 gccggagaga caatcgaggt cgatctgggc gatgtatttg cggctaattt tcgtgttgta
     4801 gggcataagc ctcttggggc cagaacggct gaactcgcaa tccctgtcag gtgtaacact
     4861 ggaaacgctg ggttagttaa ggtcaacctg agtctgacgg caacttcaga ccccagctat
     4921 ccccaggcga ttaagacttc acgtcctggc gtgggcattg tggtgaccga tagccagaac
     4981 aacattattt ccccggcggg tggaacacta ccgctctcta ttcctgatga tgcagacagt
     5041 atcgcgcgaa tgaatgtcta tccggttagc acgacaggcg taccaccaga aaccgggcga
     5101 tttgaagcca cggcaacggt gagaataaat tttgattaac gcgtgaacgt atgaagaaaa
     5161 aaacaatatt tcagtacgtt atcttgttct ttagctttct taacacccat gtcgggatgg
     5221 ctgggcctga acaagtcagt atgcatattt atggtaatgt ggtcgatcag ggctgtgatg
     5281 ttgccaccaa aagcgcatta caaaatattc atattggcga ttttaatatc agtgattttc
     5341 aggctgcgaa taccgtaagc acagctgctg atttgaatat cgatatcacc ggttgtgccg
     5401 ctggtattac tggcgcggac gtccttttta gcggcgaggc tgacaccctt gcgccgacac
     5461 tactcaaact aactgacaca ggcggaagcg gtggtatggc aacggggatt gccgtgcaaa
     5521 ttcttgatgc gcaaagtcag caagaaatcc cgctcaatca ggtccagcct cttacgccct
     5581 taaaagcggg ggataacaca ctcaaatatc aacttcgtta taagtccaca aaggcggggg
     5641 ctacgggcgg taatgcgacg gcggttctct attttgatct ggtttaccag tgaaggagtg
     5701 atgttgaaac ggataatctg gattctgtgc ttactgggtt taacggggag ttgtgagcta
     5761 tttgcccatg atggcacggt caacatcagc ggatcgtttc gccgtaatac atgcgtgctg
     5821 gcacaggata gcaagcaaat caatgtgcag ttaggcgatg tctcgctgac acgtttttct
     5881 catgggaact atggcccgga gaaatctttc atcattaatt tgcaggattg cggaacagat
     5941 gtcagcacgg ttgacgttac cttttcagga acacccgacg gaatgcagag cgaaatgctg
     6001 tccattgaaa gtggtacgga tgcagccagt gggctggcaa ttgctattct ggatgatgtg
     6061 aaaatattga ttccgcttaa ccaggcgagt aaagactaca gcctacatag cggtaaggtg
     6121 ccattaactt tttatgcgca attgcgacct gttaatagcg atgtgcagtc gggtaaagtg
     6181 aatgccagcg cgacatttgt acttcattat gactaatacg tggaacagat tgacgctctc
     6241 gatttttgcc gttttatcgc tgctggtggc gggggaattg caggcaggag tggtggttgg
     6301 cggaacgcga tttatcttcc cggcagacag agaatcgata tctatttcac tgactaatac
     6361 cagtcaggat tcctggctta ttaacagtaa aatcaatcgc ccaacgcgtt gggccggggg
     6421 ggaagcgtcg acagtgccag caccattact ggccgctccg ccacttattc tcctgaagcc
     6481 cggtactaca ggcacgttgc gcttgctgag aacggaaagc gacatcttgc ctgtggatcg
     6541 cgaaacgcta tttgagttaa gcattgccag cgtaccatcc ggcaaagttg aaaatcagag
     6601 cgtaaaagtg gcgatgcgct cggtatttaa actgttctgg cgacccgaag gtttgccggg
     6661 cgacctgctg gaagcttatc aacaattacg ctggacacgg aattcgcagg gtgtacaact
     6721 cactaaccca acgccttact acattaacct gattcaggtg agcgtaaatg gtaaagcgtt
     6781 aagtaatgcg ggagttgtgc cacctaaaag ccagcgtcag acaagctggt gtcaggcaat
     6841 agcaccgtgt catgtctcct ggcggacgat aaatgattac ggcgggttga gtgcaaagaa
     6901 ggagcaaaat ctgctctgaa acaggctcgg aaaacgtttg cgcttttttt gccacaggtc
     6961 aattcccttt tggtccgaac tcgcacataa tacgcccccg gtttgcacac cgggaatcca
     7021 ggagagttca tgtactaccc cttcgttcgt aaagcccttt tccagctcga tccagagcgc
     7081 gctcatgagt ttacttttca gcaattacgc cgtattacag gaacgccgtt tgaagcactg
     7141 gtgcggcaga aagtgcctgc gaaacctgtt aactgcatgg gcctgacgtt taaaaatccg
     7201 cttggtctgg cagccggtct tgataaagac ggggagtgca ttgacgcgtt aggcgcgatg
     7261 ggatttggat cgatcgagat cggtaccgtc acgccacgtc cacagccagg taatgacaag
     7321 ccgcgtctct ttcgtctggt agatgccgaa ggtttgatca accgtatggg ctttaataat
     7381 cttggcgttg ataacctcgt agagaacgta aaaaaagccc attatgatgg cgtcctgggt
     7441 attaacatcg gcaaaaataa agatacgccg gtggagcagg gtaaagatga ctatctgatt
     7501 tgtatggaaa aaatctatgc ttatgcggga tatatcgcca tcaatatttc atcgccgaat
     7561 acaccgggat tacgtactct gcaatatggc gaagcgctgg atgatctctt aaccgcgatt
     7621 aaaaataagc aaaatgattt gcaagcgatg caccataaat atgtgccgat cgcagtgaag
     7681 atcgcgccgg atctttctga agaagaattg atccaggttg ccgatagttt agttcgccat
     7741 aatattgatg gcgttattgc aaccaatacc acactcgatc gttctctggt tcagggaatg
     7801 aaaaattgtg atcaaaccgg tggcttaagt ggtcgtccgc ttcagttaaa aagcaccgaa
     7861 attattcgcc gtttatcaca ggaattaaat ggtcgcttac cgatcatcgg tgttggcggc
     7921 atcgactcgg ttatcgctgc gcgtgaaaag attgctgcgg gggcctcact ggtgcaaatt
     7981 tattctggtt ttatttttaa aggtccgccg ctgattaaag aaatcgttac ccatatctaa
     8041 ttatttcttc gaattcctct ttataagata atcagggctt tattttaagc cctggttgtt
     8101 ttatattcat cgctgttgct tatttagaca ttttgtactt ttattgttga ggttattaag
     8161 cgaagcgaca atggattgtg ttgctgcggt tttatagggt aggggagagg caaatgcgaa
     8221 ttaaaccaga tgataactgg cgttggtatt acgatgaaga gcacgatcga atgatgctcg
     8281 atttagccaa tggtatgcta tttcgctcac gttttgcgcg caaaatgttg actccagatg
     8341 ccttttctcc cgcaggcttt tgcgttgacg acgccgcgct ctatttctca tttgaagaaa
     8401 aatgccgcga ttttaattta tctaaagaac aaaaagccga actggtgctt aatgcgctgg
     8461 tagcaattcg ttatctcaaa ccgcaaatgc cgaaaagctg gcattttgtt tcccatggtg
     8521 aaatgtgggt gcccatgccg ggcgatgcgg cctgcgtttg gttgagcgac actcacgagc
     8581 aagtcaattt gctggtcgtt gaatctggcg aaaatgccgc actatgcctg ctggcacaac
     8641 cctgcgttgt tattgcgggt cgcgcgatgc agctgggcga cgccatcaaa atcatgaacg
     8701 acaggctgaa accgcaggtt aatgttgaca gcttcagcct cgaacaggca gtctaacgcg
     8761 tcagcttaag cgcagtcttc ggtacacagc tacagcaaag aatggtgcca tcatcgccca
     8821 ttgctgattt tttcagcggc gtgacttcgc cttctaaaag ctgaacacgg caacttccac
     8881 aaatgcccgc gcggcaagaa taagggatac gaattccctg attttctaat tgctccagca
     8941 acacttgttg gttatttcca cgaaatgcct gtccctgcca gtcaatatct acatttgcgt
     9001 ccggttgttg cgtgatgttg gcggtatcat cagcggcagc tgcgccgtaa atttttgccg
     9061 gagccgttgc cagaatttcc acctcatcgc caacgcgaat cacgccgcta ttacgggcaa
     9121 ttaaattctg accaaaatcg acatcgccgt tatcctgggc agtgcggaaa gattgcaatg
     9181 tttttaatgg ttcgcctgcc ggatgttttt gccctttttc tgggctgacg gtggtgaaaa
     9241 tacagcggct acaaggttta accacatcaa ataccacatc accaatgcga atcactttcc
     9301 agcgatcttc ttcccaggct gacgcgccgg aaaccaccag attagggcgg aattgctcca
     9361 tttttacact ggccggacaa cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa
     9421 ggtaaggata gccatcagca aatgacagag gtacagtgtt gtggcgtttc acgcgccggg
     9481 tcatttgtgg ccccacccag cgcaactgca cttcgcggga gaaaaatcca cttagccatt
     9541 tgttgatcgc gtctggtgca attcgcgcgg taaaatgtgt gccccaaact tcggttggtg
     9601 cgtcttgtgt ggcgaaatca gcaaaacgaa catatgcgct actgccatct ggtgcggtga
     9661 gatgcaagcc atcatgcaca ggtgaaggag taaaccgtac catctgggga aactggcgag
     9721 cggtaataaa cgtaccgtca ggttccgtga tcataaagat gcgatcgaag gccaggccac
     9781 tgatatctgc cagggcatgt gtaagaccaa tgccgcgcat cgatttaaca ggatgaacaa
     9841 aaagccgggt taatgtcgcc acgatgcggt cctcaaatga aaataagccc tcaactttat
     9901 gacatgacgc gcttattagc tataatgcgc aacaattttc ttagcgcctg tcccattagg
     9961 ctattttatt tgtcagtttg gcccagggaa gtgctcgaaa tcctcacgta cttgtgtacg
    10021 ctccggtttc tccgcgctgt ccatgtccag actgactgca acaattacgc ctactgcggt
    10081 aggctcttag agtaaaagtg acgatatgaa ttctctgttt gccagtacgg cccgtgggct
    10141 ggaagagctg ttaaaaactg aactggaaaa cctgggggcc gttgaatgcc aggtggttca
    10201 gggtggggtc catttcaagg gcgacacacg gcttgtttac cagagcctga tgtggagccg
    10261 cctggcctcg cgtattatgt tgccgctggg cgagtgtaag gtttacagcg atttagacct
    10321 ctatctcggt gttcaggcga tcaactggac agagatgttt aatcctggcg cgaccttcgc
    10381 tgtccacttc agtggtttga atgacaccat ccgcaacagt cagtacggtg cgatgaaagt
    10441 gaaagacgcg attgtcgatg ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg
    10501 cgatgcgccg gatattcgcg ttaacgtctg gctgcataaa gaaaccgcca gtatcgccct
    10561 tgatctcagt ggtgatggtt tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc
    10621 gatcaaagaa accctggcag ccgcgattgt tatgcgatcc ggctggcagc caggaacacc
    10681 gctgctcgat ccgatgtgtg gttccggtac gttgttgatt gaagcggcga tgctggcgac
    10741 cgatcgcgca ccaggcttgc accgtggacg ttggggcttt agcggctggg cacagcatga
    10801 tgaagccatc tggcaggaag tgaaagcaga agcgcaaacc cgcgcccgta aaggcctggc
    10861 tgagtatagc tctcattttt acggttcgga cagcgacgca cgggtgattc aacgtgcgcg
    10921 cactaacgcc cgtcttgcgg ggattggtga actgatcacc tttgaggtga aagatgtcgc
    10981 gcaactgacc aatccgctgc cgaaagggcc gtacggtaca gtgttgagca acccgcctta
    11041 cggcgaacgt ctggacagcg aaccggcgct gattgcgctg catagcctgc tgggccgaat
    11101 catgaaaaac cagttcggcg gctggaatct ctctttattc agtgcctcgc cggatctgtt
    11161 aagttgcctg cagttgcgtg cagacaaaca gtacaaagcg aaaaacggtc cattagactg
    11221 cgtgcagaaa aattaccacg ttgccgaaag cacaccagac agcaaaccgg caatggtggc
    11281 agaagactac gccaaccgtc tgcgtaagaa cctcaaaaaa ttcgagaagt gggcgcgtca
    11341 ggaaggcatt gaatgctacc gcctgtacga cgccgatcta ccggaataca acgttgccgt
    11401 tgatcgttat gccgactggg tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc
    11461 gcacaaagcg cgtcagcgtc tgttcgatat tatcgctgca accatttcgg tactggggat
    11521 tgcgccaaac aaactggtgc tgaaaacccg tgaacgccag aagggcaaaa atcagtacca
    11581 gaaactgggc gagaagggcg agtttctcga agttaccgaa tataacgctc acttgtgggt
    11641 gaacctgacg gattacctcg ataccggtct gttcctcgat caccgtatcg cccgtcgtat
    11701 gctcggtcag atgagcaaag gcaaagattt cctcaacctg ttctcttata ccggcagcgc
    11761 caccgtgcac gcgggattag gcggtgcacg cagcactacc accgtggata tgtcgcgtac
    11821 ttatctggag tgggcagaac gcaacctgcg tctgaatggc ctaaccgggc gtgcgcatcg
    11881 cctgattcag gccgattgcc tggcgtggct gcgtgaggct aatgaacagt tcgatctgat
    11941 ctttatcgat ccaccaacct tctctaactc gaaacgaatg gaagatgcgt ttgatgttca
    12001 gcgcgatcat ctggcgctga tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat
    12061 catgttctcg aacaacaaac gcggtttccg tatggatctt gacggactgg caaaactggg
    12121 actgaaagca caagaaatta cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca
    12181 gatccacaac tgctggctga ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt
    12241 atgcatggcg catggctgtc gttcagcgac gcgccgcttc tcgataacgc agaactgcat
    12301 atcgaagata acgaacgtgt ttgtctggtg ggccgcaacg gcgcaggcaa atcgacgtta
    12361 atgaaaatcc tcaaccgtga acaagggctg gatgacggtc gcattattta cgagcaagat
    12421 ttgattgtag ctcgtctgca acaggatccg ccgcgtaacg ttgaggggag tgtttatgat
    12481 ttcgttgccg aaggcattga agaacaagcg gaatacctga aacgctatca cgatatttcg
    12541 cgcctggtga tgaacgaccc gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa
    12601 cagctggatc accacaacct gtggcagctg gaaaaccgca ttaacgaagt gctggcgcaa
    12661 ctggggttag atcctaacgt tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg
    12721 gcattaggac gcgcgctggt gagcaatccg cgcgtattgc tgctcgacga accgactaac
    12781 cacctggata tcgaaaccat cgactggctg gaagggtttc tcaaaacctt caatgggacg
    12841 attattttca tctcccacga ccgttcgttt atccgcaata tggcgacgcg tattgttgat
    12901 ctcgatcgcg gcaagctggt gacctatcca ggaaattacg accagtacct gctggaaaaa
    12961 gaagaagccc tgcgcgtgga agaactacaa aatgccgagt tcgatcgcaa actggcgcag
    13021 gaagaggtgt ggatccgcca ggggatcaaa gcacgccgta cccgtaatga aggccgcgta
    13081 cgcgccctga aagcaatgcg tcgcgagcgt ggcgaacgtc gcgaagtgat gggtaccgca
    13141 aagatgcagg tggaagaggc cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt
    13201 tgctaccagg tcgacggtaa gcaactggtg aaagattttt ctgcccaggt tctacgtggc
    13261 gacaaaattg ccctgattgg tccgaatgga tgcggcaaaa ccacgctgct gaaactgatg
    13321 ctcggtcagc ttcaggcgga cagcgggcgt attcacgttg gcaccaaact ggaagtggct
    13381 tatttcgatc agcaccgtgc ggaactggat cccgataaaa cggtgatgga taaccttgct
    13441 gaaggtaagc aagaggtgat ggttaacggc aagccacgcc atgttttggg ctatttgcag
    13501 gacttcctgt tccatccgaa acgggcgatg acgccggtac gtgcgctttc tggcggtgag
    13561 cgtaaccgct tgttgttggc gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac
    13621 gaaccgacca acgatcttga tgttgaaacg ctggaactgc tggaagaact gatcgacagc
    13681 taccagggca cggtattgct ggtgagccac gatcgtcagt ttgtcgataa caccgttaca
    13741 gaatgctgga tcttcgaagg cggcggtaaa attggtcgtt atgtcggcgg ttatcatgat
    13801 gcccgtggtc agcaagaaca gtatgtggcg ctcaaacagc ctgcggtgaa aaaaatcgaa
    13861 gaagccgccg cgccaaaagc agaaactgta aaacgcagca gtagcaaact aagctataaa
    13921 ttgcagcgcg aactggagca gctaccgcaa ttgctcgaag atctggaggc gaagctggaa
    13981 gccctacaga cgcaggtggc ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa
    14041 aaagtgctcg ccgatatggc tgctgcagag caggagctgg agcaagcctt tgaacgctgg
    14101 gagtatcttg aagcgttaaa aaatggtggc tgatcgcaaa atagtcgatt taaacagaga
    14161 cagctgacaa cggtaaaatt gcctgatgaa ctgggtttat caggcatgaa aaccaacact
    14221 ttgttagtca tctgtaaaat agcgcatcat taaggagtac caatgtgcga acatcatcat
    14281 gccgcgaagc acatcctgtg ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag
    14341 catggtcaga aagcggcatg tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc
    14401 cctcggcagc gtcccaccgc ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac
    14461 ttgtttcctt ttgtgaatat gaacgttgca ggagttacca gtgaaattac attactggaa
    14521 attcccggcg tgcttttttc tgaggactac gccagcctcg gcaccttttt cctgttgttt
    14581 gtgcaactgg ttcccgcgtt ttgtctgata accattctgt tactggtgaa tcgcgcggaa
    14641 ttaccggtcc gtttaaaaga gcaactggca cgggtgcttt ttcaactcaa aacctgggga
    14701 atggcggaga ttttcctcgc gggtgtgctg gtcagtttcg ttaaactgat ggcttacggc
    14761 agcattggtg taggcagcag ctttctccca tggtgtttat tttgtgtcct gcaactgcgc
    14821 gcttttcagt gcgttgatcg tcgctggtta tgggatgaca tcgccccgat gccagaactg
    14881 cgccagccgc taaaaccagg cgtcacgggg atacgtcagg ggctgcgttc atgctcctgt
    14941 tgtacggcaa tccttcctgc tgacgaaccc gtatgcccgc gctgtagtac caaagggtac
    15001 gttcggcgta gaaacagcct gcaatggaca ctcgcgctgc ttgttacgtc catcatgctg
    15061 tatcttccgg ctaatatttt gcccatcatg gtgacggatt tattaggctc gaagatgccg
    15121 tcgacgattc tcgctggggt catcctgtta tggagcgaag gatcttatcc cgtcgctgcg
    15181 gttatctttc tggccagtat tatggtgcca acgttaaaga tgatcgccat agcgtggttg
    15241 tgttgggatg ccaaagggca tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa
    15301 gttgttgagt ttgtaggccg ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg
    15361 gcgctggtgc gtatgggagg tttaatgagt atttatccgg caatgggtgc attaatgttt
    15421 gctttagtcg tcataatgac aatgttttct gctatgacgt ttgacccacg tttgtcgtgg
    15481 gatcgtcaac ctgaatcaga gcatgaggag tcctgacagt atggaatcta ataatgggga
    15541 agccaaaatc cagaaagtga agaactggtc tcccgtgtgg atatttccta tcgtcacggc
    15601 gctcattggg gcctgggttc ttttttatca ttacagccat caggggccgg aagtgaccct
    15661 gatcaccgcg aatgcggaag gaatagaagg tggtaaaacc accattaaaa gccgtagcgt
    15721 tgacgtcggc gtggttgaaa gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa
    15781 agcgcggctg aattccggta tggaaaaatt gctgcataaa gataccgtct tttgggtggt
    15841 taaaccgcag attggtcgag aagggattag cggcctggga acgctgctgt ctggtgttta
    15901 tatcgaactg cagccaggcg cgaaaggcag caaaatggat aaatacgatt tgctggactc
    15961 accaccgttg gccccgcctg atgcgaaagg tatccgtgtg attctcgata gcaaaaaagc
    16021 cgggcagctc tcgccaggag atcccgtgct gttccgtggc tatcgggtag gttcggttga
    16081 aaccagcacc ttcgatacgc aaaaacgcaa tatcagctat caactgttca tcaatgcacc
    16141 ttatgaccga ctggtgacca gcaatgttcg cttctggaaa gatagtggca ttgcggttga
    16201 tctgacgtca gcggggatgc gtgtggagat gggctcattg acaacgctgc ttagtggcgg
    16261 tgtcagcttt gatgtgccgg aaggtctgga tttagggcag ccagtggcac cgaaaacagc
    16321 ttttgttttg tatgatgatc agaagagcat tcaggattcg ttgtacaccg atcacattga
    16381 ttatctgatg ttctttaaag attcggtacg cggtctgcaa ccgggagctc cggtagaatt
    16441 ccggggtatt cgcctgggta ccgtaagcaa agtgccattc tttgcgccga atatgcgtca
    16501 gacatttaac gatgattacc gtattccggt actgattcgt atcgagccag agcggctgaa
    16561 aatgcagctt ggcgaaaatg cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg
    16621 tttacgcgga tcgctgaaaa ccggaaacct ggtcactggt gcactgtatg ttgatctcga
    16681 tttctatcca aatacgcctg caataaccgg tattcgtgaa tttaatggtt atcagattat
    16741 cccgaccgtt agcggcggtc tggcgcaaat ccagcaacga ctgatggaag cgttggataa
    16801 gatcaacaaa ctgccattga atccgatgat tgaacaggca accagtacgc tttctgaaag
    16861 tcagcgcaca atgaaaaacc tgcaaacgac gctggatagc atgaacaaga tcctcgccag
    16921 ccagtcgatg cagcagttac cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag
    16981 tatgcagggc ttccagcctg gctccgcagc ctacaacaag atggtggcgg atatgcagcg
    17041 acttgatcag gtgttgcgag aactgcaacc ggtgctgaaa acgctcaacg agaagagtaa
    17101 cgcgctggta tttgaagcga aggacaaaaa agatccagag ccgaagaggg cgaaacaatg
    17161 aaaaagtggc tagtgacgat tgcagcactg tggctggccg gatgcagctc cggcgaaatt
    17221 aataaaaact attaccagtt acctgtggtg cagagcggta cacaaagtac cgccagccag
    17281 ggtaatcgcc tgttatgggt agagcaggtc actgttcctg actatctggc gggaaatggt
    17341 gtggtttatc aaaccagtga tgtgaagtat gtgattgcca acaacaactt gtgggccagc
    17401 ccattggatc aacagttgcg caacaccctg gttgccaacc tgagcacgca actgcccggc
    17461 tgggtggttg cctcccagcc tctgggaagc gcccaggaca cgctcaatgt taccgtaacg
    17521 gagtttaacg gtcgctatga tggcaaggtc attgtcagtg gggagtggct gttgaaccac
    17581 cagggacaac tgatcaaacg tccgttccgt ctggaagggg tgcaaactca ggatggttac
    17641 gatgaaatgg ttaaagtgct ggccagtgtc tggagtcagg aagccgcttc tattgcgcaa
    17701 gagataaagc gtctacctta attataaaga tttgtaaata taaccgcctc cggtatgttg
    17761 cctgaggcgg tttttttgtt tctaaggtgc ggaaaaagtt gttcctcttc acattttttg
    17821 tacaactgac atgcccgtgt agctcacaaa tatgacagtg gcgtgaattt tgcgcattga
    17881 cggcagttat gattcgcggt attgcttaac tgtgattgca catttagtaa tcactgtttt
    17941 cttttccacc agaaaccagt atgagggaaa cgaggcatga agagacaaaa acgagatcgc
    18001 ctggaacggg cacatcaacg tggttatcag gccggcatcg ccggacgctc aaaagaaatg
    18061 tgtccctatc agacgctgaa tcaaaggtca caatggctgg gaggctggcg agaagccatg
    18121 gcggacaggg tagtaatggc ctgattctgt
//
LOCUS       DAOTRK010000095        18094 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.095, whole genome shotgun sequence.
ACCESSION   DAOTRK010000095 DAOTRK010000000
VERSION     DAOTRK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.095"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..470)
                     /locus_tag="QIE41_003098"
                     /pseudo
     CDS             complement(1..470)
                     /locus_tag="QIE41_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            512..5005
                     /locus_tag="QIE41_003099"
     CDS             512..5005
                     /locus_tag="QIE41_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HDS3531960.1"
                     /translation="MAALPEYSLVGVQDGSHGVVNVTDKGHWNFLGTGEAFRYIYIGD
                     AGDGELNVSSEGKVDSGIITAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNIS
                     NEGLVVSNGGSSLGYGETGVGKVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFV
                     SQNITFLGDKASGIGTLNLMDATSLFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIG
                     GNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGAGELNITTGGIVKARDTQIALND
                     KSKGDVRVDGQNSLLETFNMNVGTTGTGTLTLTNNGTLNVEGGEVYLGVFAPAVGTLN
                     IGTAHGEAAADAGFITNATKVEFGLGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVM
                     HDAGHTVFNAGNTYSGKTLVNGGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTN
                     SAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHAAGTEFAGVAQLKDSTFALERDNTAA
                     LTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLTFDTDIPAATLAEGYISVDTLVVGA
                     GDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLNLLEHDDSHVGVQLVKAQTV
                     IGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKAL
                     NIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATYVQM
                     GTLRTDADGALGNTRELNISNAAIVDLNGSEQTVEAFTGLIGSTVLFKEGSLTVNKGG
                     ISQGELTGGGNLNVTGGTLAIEGLNARYNALTSVSPNAEVSLDNTQGLGRGNIANDGL
                     LTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVGQFNIDTGSALSVNEQKNLG
                     DASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILTLNKDSAAYQGTTDIVGGEI
                     AFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGGTLRVAKTTVGGNLENGGTV
                     QMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDN
                     IGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKW
                     DGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYT
                     DSLHSQGSASSMWMRHVGGHERFRTGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHL
                     GVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWF
                     DNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWYVQPQVQITWMGVK
                     DSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKV
                     YAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVK
                     YSW"
     gene            5189..5455
                     /gene="hipB"
                     /locus_tag="QIE41_003100"
     CDS             5189..5455
                     /gene="hipB"
                     /locus_tag="QIE41_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDS3531961.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5455..6777
                     /gene="hipA"
                     /locus_tag="QIE41_003101"
     CDS             5455..6777
                     /gene="hipA"
                     /locus_tag="QIE41_003101"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDS3531962.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            complement(6977..8329)
                     /locus_tag="QIE41_003102"
     CDS             complement(6977..8329)
                     /locus_tag="QIE41_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003862286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDS3531963.1"
                     /translation="MDFSEYQTEIIEDIKSCLEGLQVQPILFMGAGVSQRYLKAPDWE
                     NLLKYLAETCPLITRPYGYYSQTRNDNKPMIASDFSESYAEWAWDVGTSRYPEQYFNG
                     KSSKDIYIKHEIATLLNNLSDSVDFSAMEYTEEIEKLKKVRPHAIITTNYDDLLEKIF
                     DNYQPIVGHNVVTVNYTTYGEIMKIHGSSKEPESIIITNEDYVEFYKRKKYISAKLLT
                     YFVEHPLFFIGYSINDENIKAILSDIDEIIAPNNALIPNIYLVSFSPECEGTGSHQKE
                     ILIGVGENKSIRIKVIYANDFGWIYDALSSNTPEISVNPKLVRALLARTYTFASQSLV
                     KQELPYDFEMLKNIAEQDNVLAKLYGIAELNNGQALNASYPYTISELAKLLDMGSWHY
                     VHKVLERIHKETGFNIKSSDNNYHVLVKTGKEGVHKYSPAAFELIKKIINGQKYKLKP
                     "
     gene            complement(8544..9576)
                     /locus_tag="QIE41_003103"
                     /pseudo
     CDS             complement(8544..9576)
                     /locus_tag="QIE41_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(9943..10842)
                     /locus_tag="QIE41_003104"
     CDS             complement(9943..10842)
                     /locus_tag="QIE41_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3531964.1"
                     /translation="MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALKLLEE
                     YIGHPLFVRTTRRIELTETGQLLLEQTAAAVESLENSLESIRDQHQEPSGMVRITLSR
                     FAYLLILKPAMAEFCQQYPGIQLEISVYDGTVNIIKERFDLGIRFGDILEGGVVARPL
                     MKPFREGLYASSAYLSRHGTPEVPADLCHHQLIGYRFITNNRILPLLLNDRGEQLTVE
                     MPGQLISNDIDVMADGIRNGLGIGRLFEPVWQLQPDRERFIPVMERYWKTYPPVYLYY
                     PKNAGKTKRVKALIDFLISTTGR"
     gene            10981..11289
                     /locus_tag="QIE41_003105"
     CDS             10981..11289
                     /locus_tag="QIE41_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDS3531965.1"
                     /translation="MRILCLDIPAPGASLEKYAPHLNAEALHAWGLYKSGFIRDIYFR
                     QDRPGVAIFLECDSVDEAMNVMAEFPLAKAGLLSFECIPLGSFINWENLFADEFKNKE
                     "
     gene            11298..11987
                     /locus_tag="QIE41_003106"
     CDS             11298..11987
                     /locus_tag="QIE41_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDS3531966.1"
                     /translation="MKPAYKPVLCVVSSHPIKGASGVPTGFFLAELTHPLKVLEDAGI
                     KTTIASIRGGQPPVDGFDLSDPVNAWYWNETDFQQRLATTPALSELKGADYSAVFFAG
                     GHGTMWDFRDNQDAQRIIREVYESDGIVSAVCHGPAALVDAKLSSGEYLIKGKNVAAF
                     TNKEEEEVHTTDVVPFLLETALREHGALYHEAPNWSENVVTDDRLITGQNPASAHGVG
                     VALLNALRRTA"
     gene            12061..13008
                     /locus_tag="QIE41_003107"
     CDS             12061..13008
                     /locus_tag="QIE41_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS3531967.1"
                     /translation="MKTLPPVALGTWSWGTGFAGGDTVFGNHLSDTQMAEVFTAAMSK
                     GLNLWDTAAVYGMGSSETALGALVRQFPRENIILSTKFTPQIADKQSAQPVSDMLEAS
                     LGRLGVDAIDIYWIHNPLDVEKWTPGLIPLLQSGKVKRVGVSNHNLAQIRRANEILNA
                     SGYSLSAVQNHYSLLYRASEEAGILDYCRQNNITFFAYMVLEQGALSGRYDSNHPMPA
                     GSGRAESYNAVLPQIERLTAAMKKMGAERNASVAQIAIAWAIAKGTLPLVGATKVHHV
                     LDAACASDIQLRDEEIILLEQLAAETRVDTRGAWEKPMV"
     gene            13111..13788
                     /locus_tag="QIE41_003108"
                     /pseudo
     CDS             13111..13788
                     /locus_tag="QIE41_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            13939..14511
                     /locus_tag="QIE41_003109"
     CDS             13939..14511
                     /locus_tag="QIE41_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDS3531968.1"
                     /translation="MKNILVISGHPELTHSVANATILDEVETALPDVEIRRLDRLYPD
                     GKFNIAAEQKSLLRADVIVWQFPFSWYGLPGLMKKWLDEVFVHGFAHGSTAKLGGKKL
                     ILSFTTGAPEELYTVDSFFGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDAD
                     KIAQQKTLAREHASRLVELLNSVVNNPEGE"
     gene            14514..15533
                     /locus_tag="QIE41_003110"
     CDS             14514..15533
                     /locus_tag="QIE41_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS3531969.1"
                     /translation="MQYTRLGKSDLLVSRICMGCMGFGDPSTGQHSWTLDETASRDII
                     RYGLEKGINFYDTAIAYQNGSSERYVGRALREMAKRDDIVLATKFLPRTPAQIAGGIS
                     GKEAIAQSLDQSLKNLGMDYIDLYIYHIWDYNTPIIDVLEALHTAVTAGKVRSVGISN
                     CYAWQLAKANALAEREGLMPFVSVQSHYNLIMREDERELFGLCTEDNIAMTPYSALAS
                     GRLSRKEGYTRRATEDDYARGKYDSTAEQDRSIIERVAELAERHLVSMTEISLAWLLT
                     KVTSPVVGATKKAHVDGAVNAVNLQLSPEEIQFLEETYQPHVLTGVMAQNTPQTKDVK
                     QVWMR"
     gene            15679..16374
                     /locus_tag="QIE41_003111"
     CDS             15679..16374
                     /locus_tag="QIE41_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDS3531970.1"
                     /translation="MQISSPVFATKPDAPNKPEMNIFELGIRPDRDNDFADVARQTIS
                     ASVDHEAGTLAMYALQRSDNSGQAFMFELYENERAYRKHLNAAPYKAFAARAPDIIDR
                     KKKITLEPQFLGDKHIIPNERTINNLVIVEVKPEFQSEFRNIVLPEMVESLKVEKGVL
                     AMYAGTDSETPKRWYFYEIYASEEDYQLHRQTLHFLDYLRQTAHMSARKDAIPVKPVF
                     LRNKGGIKQEPQR"
     gene            16390..17193
                     /locus_tag="QIE41_003112"
     CDS             16390..17193
                     /locus_tag="QIE41_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDS3531971.1"
                     /translation="MKSVLAAAAMSLVISDFANAEETRGKAMMKIEPSAISEADIRSV
                     SPALARFGREAITEDLWTRDALSPRDRSVVTVAMLIARNQPAELKHYIDVALDSGVTP
                     AELSEIITHLAFYSGWPNAMSAVSVTKAVFETRGVTPDALPDASPDLLPLNQEAEKQR
                     SETVEKNVGPISPGLVKFTADPLFLDLWQRPALKPRDRSLITVSALIASGQSAQIGYH
                     LNRAMDNGLSVEEAGEIVTQAAFYAGWPNAFTAAPVVGEVLNNRSSSKR"
     gene            17596..17862
                     /locus_tag="QIE41_003113"
                     /pseudo
     CDS             17596..17862
                     /locus_tag="QIE41_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         4770 a         4260 c         4555 g         4509 t
ORIGIN      
        1 ggccgacagc aggctcagaa gacgctccag tgtggccaga gtgcgttcac cgaagacgtg
       61 cgccacaacc gtcctccgta tcctgtcata cgcgtaaaac agccagcgct gacgtgattt
      121 agcaccgacg tagccccact gttcgtccat ttcagcgcag acaatcacat cactgcccgg
      181 ttgtatgcgc gaggttaccg actgcggcct gagtttttta agtgacgtaa aaccgtgttg
      241 aggccaacgc ccataatgcg tgcactggcg cgacatccga cgccattcat ggccatatca
      301 atgattttct ggtgcgtacc gggctgagag gcggtgtaag tgaactgtag ttgccatgtt
      361 ttacggcaat gagagcagag atagcgctga tgtccggcag tgcttttgcc gttacgcacc
      421 acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac gcaagcacct
      481 taaaatcacc atcatacact aaatcagtaa gttggcagca ttaccagaat attcattggt
      541 gggcgttcag gacggttccc acggtgtcgt caacgtgact gataaagggc attggaattt
      601 cctcggaacg ggcgaagctt tccgctatat ctatatcggt gatgctggcg atggtgaact
      661 taatgtctcg agtgaaggca aagtagattc gggaattatc actgcgggga tgaaagaaac
      721 aggcacaggc aacattactg ttaaggataa gaactccgtt atcactaatc tcggaactaa
      781 tcttggttat gacggccacg gcgaaatgaa tatcagtaat gaggggcttg ttgtcagcaa
      841 cggaggaagc tcactcggtt atggagaaac cggcgtcggg aaggttagca tcaccacggg
      901 ggggatgtgg gaggtcaata agaatgtcta taccactatt ggtgttgcgg gcgtcggaaa
      961 cctcaatatc agcgatggcg gtaagttcgt atcgcaaaat attacttttt tgggcgataa
     1021 agcaagcggt atcggcacac tgaacctgat ggatgcgaca tcattgttcg atactgtagg
     1081 tatcaatgtc ggtaattttg gtagcggcat cgtaaatgtc agtaatggtg ccactcttaa
     1141 ttcaacgggc tatggattta tcggaggaaa tgcctccggt aaggggatag ttaatatttc
     1201 aacggatagt ctctggaatt taaaaacgtc atctactaac gcccaattgc tacaggtcgg
     1261 tgtattaggc gcgggtgaac tgaatattac caccggaggt atagttaaag cgcgtgatac
     1321 acaaatagct ctcaatgaca aaagtaaggg cgacgtgcgg gtggatgggc agaactctct
     1381 tcttgaaaca ttcaatatga acgtagggac gactggcaca ggtacgttaa ccctgacgaa
     1441 taacggtacg ctgaatgtcg aaggtggaga agtttactta ggtgtttttg cgcctgctgt
     1501 aggaacgcta aacattggta ctgctcacgg tgaggcggcg gcagatgccg gatttatcac
     1561 caatgcgacg aaagtggagt ttggtcttgg cgaaggcgtt tttgtcttta atcataccaa
     1621 taacagtgat gccggctacc aggtcgatat gctgattacg ggtgacgata aagacggaaa
     1681 agtgatgcat gatgcaggcc atacggtgtt caatgcaggg aatacttata gcggtaaaac
     1741 gttggtcaat ggcggcctcc tgaccattgc gtcacatacg gcagatgggg taactggcat
     1801 gggttcgagt gaagtaacca ttgcaagccc cggtacgctc gacattctcg catcaacgaa
     1861 cagtgcggga gattacacgc tgaccaatgc gctcaaaggc gatggcttga tgcgagtgca
     1921 gctgtcatcc tccgacaaaa tgtttggctt tacccatgca gcagggactg aattcgccgg
     1981 tgttgcccaa ctgaaagaca gtaccttcgc actggaacgc gacaacaccg ctgcgcttac
     2041 tcacgcgatg ctgcagtctg acagtgaaaa taccacatcg gtaaaagtgg gagagcaatc
     2101 cattggtgga ctagccatga atggcggtac gctcactttc gatacggata tacctgctgc
     2161 gacgcttgcg gagggatata tcagcgtcga tacactggtt gtcggcgcgg gtgactacac
     2221 ctggaaaggc cgtaactatc aggtaaacgg gacgggcgac gtgcttatcg acgtgcctaa
     2281 accgtggaat gatcccatgg cgaataaccc tctgacgacg ctcaatttgc tggaacacga
     2341 tgatagtcat gtcggcgttc aactggtgaa ggcgcaaacg gttattgggt cgggtggctc
     2401 attaacgtta cgtgatttac agggcgacga ggtggaagcg gacaaaacgt tacatattgc
     2461 gcaaaacgga acggtggtcg ccgagggtga ttatggattc cgcctcacga ccgcaccagg
     2521 tgatggtttg tacgttaact atgggctgaa agcgctgaac atccatggtg ggcaaaagct
     2581 gacgttagcc gaacatggcg gagcctatgg cgcaacggcc gatatgtcgg caaaaatcgg
     2641 tggtgaaggg gatctggcaa tcaatacggt gcgacaggtt tcgctttcca acggtcagaa
     2701 cgactatcag ggggcaacct acgttcagat ggggacatta cgtaccgatg cggatggtgc
     2761 acttggcaac acccgggaac tgaacatcag caacgcggcc atcgtcgatc ttaatggatc
     2821 ggagcagacg gtagaggcat tcaccggact gataggttcg actgttttgt tcaaagaggg
     2881 ttcgctgacg gtgaataaag gtgggatcag tcagggcgaa ctgacaggtg gcggaaacct
     2941 gaatgttaca ggtggaacac tggctatcga ggggcttaat gcacgctaca atgcgttaac
     3001 cagcgttagc ccaaatgcgg aagtcagcct cgataatacg caggggttag gcagaggaaa
     3061 tattgccaat gacggtctgt taacgctaaa aaacgtgact ggcgaactgc gtaatagcat
     3121 aagcgggaag ggtatcgtga gcgcaacagc caggacagac gtagagctgg atggcgataa
     3181 tagccgcttt gtggggcaat tcaacattga tacaggcagc gcgctcagcg tcaacgagca
     3241 gaaaaacctg ggtgatgctt ccgttatcaa taatggcctg ctcaccatct ccactgagcg
     3301 tagctgggcg atgacgcaca gtatcagcgg tagcggtgat gtgacaaaac tgggtaccgg
     3361 gatcctgact cttaacaaag attccgcggc gtatcagggt acgacggata tcgtgggtgg
     3421 tgaaattgct ttcggttccg actctgccat taatatggca agtcagcaca ttaatatcca
     3481 taacagcggt gtgatgtcgg gaaatgtcac cactgcaggt gatgtgaacg ttatgccagg
     3541 ggggacactg cgtgtcgcta aaaccactgt cggcggtaac ctggagaatg gtggcacggt
     3601 tcaaatgaac agcgaagggg ggaaaccggg gaatgtactg accgttaacg gtaactatac
     3661 cggaaacaat ggcctgatga cgttcaacgc gacgctgggc ggcgataatt cgcccaccga
     3721 taagatgaac gtgaaaggcg atacccaagg gaatactcgc gttcgggttg ataacattgg
     3781 cggcgtcggt gcacaaacgg tcaacggtat tgaactcatt gaggtcggcg gtaattctgc
     3841 aggtaacttc gcgctgacca ccggaactgt cgaagctggg gcttacgtct acacgctggc
     3901 taaagggaag gggaatgacg agaaaaactg gtatctgacc agtaaatggg atggcgtaac
     3961 gccagcggat acacccgatc ccatcaataa tccccctgtt gtggatccgg aaggcccatc
     4021 agtttatcgc ccggaggccg gaagctatat cagcaacatt gccgcagcca actcgctgtt
     4081 tagccatcgc ttacacgacc gtctgggtga gccgcagtat acagattcac tgcattctca
     4141 gggttcggca agcagtatgt ggatgcgtca tgtcgggggg cacgaacgtt tcaggaccgg
     4201 tgacggccag ctaaatactc aggctaaccg ctatgtattg cagctaggcg gcgatttggc
     4261 gcagtggagt agcaacgcgc aggatcgctg gcatcttggc gtgatggcag gctacgccaa
     4321 tcagcacagt aatactcaga gtaatcgtgt gggttataaa tcggatgggc gcatcagcgg
     4381 ttacagcgca ggactgtacg cgacctggta tcagaacgat gcgaataaga ccggcgctta
     4441 tgttgacagc tgggcgctgt ataactggtt tgataacagc gtcagttccg ataaccgttc
     4501 tgctgacgac tatgattctc gcggtgtgac ggcctctgtt gagggtgggt atacctttga
     4561 agcgggaact tttagcggca gcgaagggac gctgaatacc tggtacgtcc agccacaggt
     4621 acaaatcacc tggatgggtg tgaaagattc tgaccatacc cggaaagacg gaacacgcat
     4681 tgaaacggaa ggcgacggaa acgtgcaaac gcgacttggg gtgaaaacct acctgaacag
     4741 ccatcaccag cgtgacgatg gtaaacagcg tgagttccag ccttacattg aagcgaactg
     4801 gatcaacaac agcaaagtct acgcagtgaa gatgaatggt caaaccgtaa gccgtgatgg
     4861 tgcgcgtaat ctcggtgaag tacgtaccgg ggttgaggcg aaagtaaata acaaccttag
     4921 cctgtggggg aatgtcggtg tgcaactagg tgataaaggc tatagcgata ctcagggcat
     4981 gctgggagtg aaatatagct ggtaaaccgt ataagccgca tgtcgagatg gcatgcggct
     5041 taagccttcc ttttatccgc gatcgcggat atcgcagcgt ttatcccgta cagcggataa
     5101 gatgtgtttc ctgattgact ttatccccac taaaggataa aactgataat atccccttga
     5161 gcggataaat atgctgtgga cgtatgacat gatgagcttt cagaagatct atagcccaac
     5221 gcaattagcg aatgcaatga aactggttcg tcagcaaaat ggctggacgc agagcgagct
     5281 ggcgaaaaaa atcggcatta agcaggcgac aatttccaat ttcgaaaaca accctgacaa
     5341 taccacgctg acgacatttt ttaagatttt acagtcgctt gaactctcaa tgacgctatg
     5401 cgacacgaaa aatgcctcgc cagaatcaac agaacagcaa gatctggagt ggtaatgcct
     5461 aaacttgtca cttggatgaa caaccagcgg gtaggcgagt taacgaagtt agccaatggc
     5521 gcgcacacct ttaagtatgc accggagtgg ttagccaacc gttatgccag accgttatca
     5581 ctttcgctgc cattgcagag ggggaatatc acctctgatg ccgtatttaa cttcttcgat
     5641 aacctgttac ccgatagccc gattgtacgt gaccggatcg ttaaacgtta tcatgccaaa
     5701 tccagacaac cgtttgattt attgtcagaa atagggcgag acagcgttgg tgccgtgacg
     5761 ttaatacccg aagatgaaac cgtaacgcat ccgataatgg catgggaaaa gcttactgaa
     5821 gccagacttg aagaagtatt aacggcttat aaagcagata tccctctggg catgattaga
     5881 gaagaaaatg actttcgcat ctcggttgct ggcgcgcagg agaagacagc gctgctcaga
     5941 ataggcaatg actggtgcat tccgaaagga ataacgccga cgacgcacat cattaaatta
     6001 ccgattggcg aaatcaggca gcccaatgcg acgctcgatc tcagccaaag cgttgataat
     6061 gagtattact gtctgctgct ggcgaaagaa cttgggttga atgtgccgga cgcagaaatc
     6121 attaaagcgg ggaatgtgcg cgcgttagcg gtcgaacgtt ttgacaggcg ttggaatgct
     6181 gagcgaacgg ttttacttcg cttgccacag gaggatatgt gtcagacatt cggtttacct
     6241 tcatcggtga aatatgaatc agatggaggc ccaggcatcg cgcggatcat ggcttttttg
     6301 atggggtcca gcgaggcgct gaaagatcgc tatgatttta tgaagtttca ggtgttccag
     6361 tggttgattg gcgcaacgga tggccatgca aaaaacttct ccgtatttat tcaggctggc
     6421 ggcagttatc gactcacgcc attttacgac atcatttcag catttccggt attgggtggc
     6481 gcaggaatac acatcagcga tctcaaactg gcaatggggg ttaacgcatc caaaggcaaa
     6541 aaaacggcaa tcgataaaat ttatccgcga cattttctgg cgacagcaaa ggtgctgaaa
     6601 tttccggaag tgcagatgca tgaaatcctg agtgactttg ccagaatgat tccggcagca
     6661 ctggataacg tgaagacttc attaccgaca gattttccgg agaacgtggt gacggcagtt
     6721 gaaaccaatg tgttgaggtt gcacggccgg ttaagccgag aatacggtat taagtaagat
     6781 atggggtttt ggtcgttgtt aatgagcagg acaatcatga ccgccaacga ctggattctc
     6841 ccttggttta tgtttgtcaa agctcagtaa aaatgtctaa tcactggctc attagaaatg
     6901 tccatatgtt gaaaaggccg ctgcgaaaat ccctcccgca acggcctctt tagcataacg
     6961 tcattatgcg aaccaattat ggtttgagtt tatacttctg accattaatg atttttttga
     7021 ttaactcgaa tgcagcgggg gaatatttat gaacaccttc cttccccgtt ttcactagca
     7081 catgatagtt attatctgag gatttaatat taaaaccagt ttccttgtgt attctttcta
     7141 atactttatg gacgtaatgc caagatccca tatcaagtaa ttttgcaagt tcagatatgg
     7201 tgtagggata gcttgcgtta agtgcttgcc cattatttaa ctctgcaatt ccataaagtt
     7261 tcgctaacac gttatcttgc tcagcaatgt tcttaagcat ttcaaaatca tatggaagct
     7321 cttgttttac aagagattga gaggcaaatg tataggttct ggctaatagc gccctgacta
     7381 attttgggtt aacactgatt tctggcgtat ttgacgatag ggcatcataa atccaaccga
     7441 aatcattggc atagatcact ttgattcgga tgcttttatt ttcaccaaca ccaatcaaaa
     7501 tctctttctg gtgtgaacct gtaccttcac attctggact aaaggaaacc aaataaatat
     7561 tcggaatcaa agcgttattt ggcgcaatta tctcatcaat atcagacagg atggctttaa
     7621 tattctcatc attaatacta tagccaatga agaacaacgg atgctcgacg aaatatgtta
     7681 aaagttttgc actgatatac tttttacgct tataaaactc aacatagtct tcatttgtta
     7741 taatgatgct ttctggttct ttactactgc cgtgaatctt catgatttca ccgtatgtag
     7801 tatagttaac agtcacaaca ttatggccaa ctatcggttg gtagttatca aatatctttt
     7861 caagcagatc gtcatagttt gttgtaataa tggcatgggg ccttactttt tttaactttt
     7921 caatttcttc agtgtactcc attgctgaaa aatcaaccga gtctgaaagg ttattcaaca
     7981 atgttgcaat ctcatgcttg atatatatat ctttagagct ttttccattg aaatactgtt
     8041 caggataacg agatgtcccc acatcccaag cccactcagc gtatgactca gaaaaatcac
     8101 ttgctatcat tggtttattg tcatttcttg tttgactgta gtaaccatac gggcgagtga
     8161 ttaatgggca agtttcagcg agatacttta acaggttttc ccaatcagga gctttcaagt
     8221 agcgttgaga tacccctgca cccataaaga gaattggctg tacctgtaaa ccttcaagac
     8281 atgatttgat atcttcaatt atttctgtct gatattcaga gaagtccata ctttagcctt
     8341 taggatgtga gatgctggag tagtaaatcg ccgcggagcg gttcggggtt ctgtgacacc
     8401 cgaattttac ctcggtttcc tttgtatata atatcattga agttatttgc atttctcaga
     8461 aatcttctct ggctttgatt gctatagccg aaagatagcg cacacttcgc cgttggtcag
     8521 ttagtacgag gtttccgcca agttcacctt ttaccaaaga tggcctcaga gcttccctga
     8581 agctttatca tggtttcaag taggtcatct tcgttcagtg agcgctgaga tttgttgatg
     8641 ctaggcgcgt gccgtctgcg ggaaggctca tcaccagaac aaggtaacga tagtgagcga
     8701 ttattgtccc gttttctctg gacctgacgg gcaacatcca acacatggcc aagtctcttg
     8761 ttatctacaa tggcgacggg ctcaacctca gaaagccggt cataggtgga gtagggtagc
     8821 agcacgccat ttagtcgcag ctccttatgt ccgtcaggat aatgccagac atcaatatac
     8881 ttgccgatgg ctcttcgact gtactcgttg tcctcaatca gatagagcat tttatcgtat
     8941 tgtacagtca gattttttga tacccggcga ggctctcgcc aggtaaattc tgcatcaagg
     9001 tcatcgtcta atgccagcgg ccggtacgcg ttaaaatcat gcaagggttc tttggcaaag
     9061 cgccggttat aatccgccat gtattcttct gcaaatgcat tggccgcttc tattgtacag
     9121 atccccttaa gcctgagctc cttaacgagt cggtcctgaa gtgtcaaatg agcacgttca
     9181 acgcggcctt ttgcctggct ggtttcagca cacaaaggag taatattaag gcagcgcatg
     9241 gccctggcaa actgggtatc gccgagaccg gtggtggcgt gcttattatt gactctgaat
     9301 acgctggctt tatcgctgta gaggatcatc ggtttaccgt attcagtggt ggtcgcagcc
     9361 atcaatttgt atcagctcgc cagtacaagg acgccggtaa cgaggctgat gaattttcgg
     9421 tgcgcgttgt ctgcggggga tccagagtcc cgcgtttatc atgagacgtc ggatggtttc
     9481 ctttccaagg accagaccgt gaacttcctc cagcttttcc cgcgccagcg tggggccaaa
     9541 atcacggtag tgctcccgga ttatactgag tgcctccaat gagccacaaa tagaacatta
     9601 gcattgagcc tctataattt tggtgcgcat aatgtatatt atgttaaatg tggtgggttt
     9661 gttcttaagt tcatgtgttc cgctctttca gtagcttaac tgatgaatca gtcagcagct
     9721 cttgccgtgc aagctacgcg gcggttcgta ccagattggc cacctccatc tcctggttcg
     9781 ctttcccctc cccccggtaa acctcaccca acgttaatta atgatgtcag tatgtgggcg
     9841 ctatccttgt atgtaaaatc cactgacgat tacgcccatc tgtgaaaaaa aactcacaat
     9901 gccattcgat gcatctccat actgaacgag gcgttatcgt ctttatcgcc ccgtagtgga
     9961 tatcagaaaa tcaatcaggg ccttcactct tttcgtttta cccgcgtttt tggggtaata
    10021 gagatacact ggcgggtagg ttttccagta tctctccatc accggaataa agcgctccct
    10081 gtccggctgt aactgccaga caggctcaaa caagcgccca attcccagcc cgttacggat
    10141 cccgtctgcc ataacgtcaa tatcgttgct gattaactgc cccggcatct caacggtcaa
    10201 ctgctcaccg cgatcgttca ggagcagcgg aaggatgcgg ttgttggtga taaaacggta
    10261 accaatcagc tggtgatgac agagatctgc cggcacttca ggcgttccat gtcgggagag
    10321 atacgctgag gatgcatata atccttcacg aaacggtttc attaacggtc gggccaccac
    10381 ccctccctca agaatatcgc caaaacggat ccccagatca aaacgctctt tgatgatatt
    10441 cacggtaccg tcgtagaccg aaatttctaa ctgtataccc ggatattgct gacagaattc
    10501 tgccatcgca ggcttaagaa ttaacaggta ggcaaaacgc gagagcgtaa ttcgcaccat
    10561 cccagagggc tcctgatgct ggtcgcgaat gctttcaagt gagttttcca gcgactccac
    10621 cgccgcagcg gtctgttcca gcaataattg accggtttcc gtcagctcta tacggcgggt
    10681 cgtacgcaca aaaagcggat ggccgatata ctcttccagc agctttagtg cattgctgac
    10741 ggagggaggc gtaatttcca gtttccttgc ggctgccgaa atattgccct cccgcgcaat
    10801 gctctggaag atgcgaatct ggttataaag agcgttattc ataatgtttt cctaagcaat
    10861 tctcgcctga ttattaagta cagtctaaaa atgcttaagt caatgaccct ctaatcttcc
    10921 tgaaatacgc ctcttatact ttagtctctt tctactacga cctgagttca ggagactatg
    10981 atgcgaattt tatgtctgga tatccctgcc cctggagcat cgctggagaa atatgctccg
    11041 cacctcaacg ctgaagcgct gcacgcctgg ggattgtata aatcaggctt catccgcgac
    11101 atctacttcc gtcaggacag acctggcgtc gctatttttc ttgaatgtga ctctgtcgat
    11161 gaagcgatga acgttatggc cgaattcccg ttggcaaaag caggcttact aagcttcgag
    11221 tgtataccgc ttggctcctt tattaactgg gaaaatctct tcgccgatga atttaaaaat
    11281 aaagagtgag gaccgatatg aaacctgcat ataaaccagt gctatgtgtc gtttccagcc
    11341 atccgattaa aggcgcatcc ggtgtaccaa ccggtttttt cctggccgag ctgactcatc
    11401 cgctgaaagt actggaagat gccggaataa aaacaacaat agccagtatt cgcggggggc
    11461 agccaccggt ggatggattt gacctcagcg accctgttaa cgcctggtac tggaacgaaa
    11521 ccgattttca gcagcgcctg gcaacaacac ctgcgttatc cgagctgaaa ggtgccgatt
    11581 acagcgcagt tttctttgca ggtggtcatg ggaccatgtg ggatttccgt gataatcagg
    11641 atgcccagcg tattatccgc gaagtgtatg aaagtgatgg gattgtctca gccgtctgcc
    11701 acgggcctgc ggcattggtt gacgcaaaac tcagtagcgg cgaatacctg ataaaaggga
    11761 aaaatgtggc ggcctttacc aacaaggaag aggaagaagt tcacacaaca gatgtggttc
    11821 ccttcctgct tgaaaccgca cttcgcgagc atggtgcgct ttatcatgaa gctccaaact
    11881 ggtctgaaaa cgtggttacc gacgatcgcc ttatcacggg gcagaatccc gcttcagcac
    11941 atggggttgg tgtggcgctg ttaaatgcac tccgccggac agcttaatta attcatcgtt
    12001 actcacctta cttggccggt ttaagtcagc cacgatccgg ccttttttca ggagctgaat
    12061 atgaaaacac tacctccagt cgcactaggt acatggtcct ggggcacagg ctttgccggc
    12121 ggcgacaccg tctttggtaa tcatctttct gatactcaga tggcagaagt gttcactgcg
    12181 gccatgagta agggcctcaa tctctgggat accgcagcag tatatggtat ggggagttcc
    12241 gagactgcgc tgggagcatt agtccgtcag tttccccgcg agaacataat tttatccacc
    12301 aaattcacgc cgcagattgc ggacaagcag tctgcgcagc ctgtcagcga tatgcttgag
    12361 gccagccttg ggcgcctggg cgtggacgcg attgatatct actggatcca taatcccctc
    12421 gacgtcgaaa aatggacacc aggactgatc ccacttttac aaagcggtaa ggtaaaacgc
    12481 gtaggtgttt ccaaccacaa tctggcacaa atcagacgcg ccaatgaaat ccttaacgct
    12541 tccggttatt ccctcagtgc agtacaaaat cactacagcc tgctctatcg cgcttctgaa
    12601 gaggccggga tccttgatta ttgccgacaa aataacatca cgttctttgc ttacatggtc
    12661 ctggagcaag gcgcgctcag tggtcgttat gattcaaatc atcccatgcc tgccgggagt
    12721 ggccgggccg aaagttataa cgcagtactg ccgcagatag aaagactaac cgccgccatg
    12781 aaaaaaatgg gagccgaaag gaatgccagc gtggcacaga tagccatcgc atgggctatt
    12841 gcgaaaggta cccttcccct cgttggtgcg actaaagtcc atcacgttct ggacgccgcc
    12901 tgcgcttcag acatccagtt acgcgatgag gaaatcatcc tgctggaaca actcgccgca
    12961 gagaccagag tggatacacg aggcgcctgg gaaaaaccga tggtgtaaag cccgtccgga
    13021 acagactgtc atgaatgaca cacacgaacg ggctgttccg ggtgattcta atctgtatca
    13081 cttttactgc tttgtatgaa tggtgagaat atgaaaatca tttgcctcga agaacattat
    13141 ctcgacagcg agttagggcg agcgtgtatg cctgttgcgc ttgagcaggc acctttcctt
    13201 ggcgacaggg ggaaaaccgt cgctgatggt cataatcctg accgactccc tgctttcccg
    13261 ctttaataaa ctgaatgtgc cactctatct tcatcccggg ttaccgctta aaagcgtaca
    13321 gcaggcctac ttcacgggct tcagcgcaga ggtcaacgcc aggctttcta tgtttgcctg
    13381 gggctggcat cacgaagcgg ggatccattt gttgcggctg atgttatcgg gcgcatttga
    13441 taaatatccc aacctgcagg taatcagtgg ccactggggg gagatgttac ccttctggct
    13501 ccagcgtctt gatgacagcc taccactggc tgcaacgggt ctgtcacgca cgctaacgag
    13561 aaccttccag gaacacgttt acgttacccc gtcatatgct aacactgccg cacttccagt
    13621 ttatctacgc gttaatgggt gctgaaagga tcctgttctc cgtcggttat ccctatcaga
    13681 cccctggacg gtgtaaaaac ctttatagac agtctgcccg tcaacaaggc tgaaaaagag
    13741 gccatcgcgt ttcgcaatgc agaacgttta ctgggcatca cggtttagtt caccgtttca
    13801 tgatgactga gcacattgtg atcagccgac aattattagt ttttgcctaa aagtgaatga
    13861 gcctttactg ctctaaaaaa ataatgttgt cactctatac tggatttaat attcaccaaa
    13921 gatgtttaac tgattgaaat gaaaaatatc cttgttattt caggtcatcc tgagcttacc
    13981 cattccgtcg ccaatgccac tattcttgat gaagtggaga ccgcccttcc cgatgttgag
    14041 attcgtcgtc tggacaggct ctatccggat ggcaaattca acatcgcggc ggagcagaaa
    14101 agcctgctca gggccgatgt gattgtctgg cagttccctt tttcctggta tgggctgcca
    14161 ggattaatga aaaaatggct ggacgaggtc tttgttcacg gcttcgcgca tggctcaacg
    14221 gcgaaactgg gcggtaaaaa gctgatcctc tcctttacaa cgggagcgcc agaggagctc
    14281 tataccgtcg acagtttctt tggccatacc attgaagagt atcttattcc gttcgaaacc
    14341 acggcaaggc tgtgcaatct tgagctgctg gcgccggttt atacctgcgg tatcagctat
    14401 gccgaccggg atgccgacaa aatcgcgcag caaaaaacgc ttgcccggga acacgcttca
    14461 aggcttgtcg aactgctcaa ttccgtcgtg aataatccag agggagaata acaatgcagt
    14521 atacccggct cggtaaaagt gacttacttg tttcccgcat ctgcatggga tgcatggggt
    14581 ttggcgatcc gtcgacgggt cagcatagct ggacgctgga cgaaacagca agccgcgaca
    14641 tcattcgcta tggccttgaa aagggcatca acttttacga taccgctata gcctaccaga
    14701 atggctccag tgagcgatac gttggccggg cattacggga gatggcgaaa cgcgatgata
    14761 tcgtcctggc caccaagttt ctgccgcgaa cccctgcaca aattgccgga gggatcagcg
    14821 gaaaagaggc aatcgctcaa tcccttgacc agagcctgaa gaatctgggg atggactata
    14881 tcgacctcta catctaccac atctgggatt acaacacgcc cattatcgac gtgcttgaag
    14941 cgttacatac cgctgttact gctggcaaag tacgatctgt tggtatttca aactgctatg
    15001 cctggcagct ggcgaaggcg aacgcccttg ctgaacgcga agggctgatg ccctttgttt
    15061 ccgtacagag ccactacaat ctaattatgc gcgaagatga acgggagctc ttcggcctgt
    15121 gcactgagga taatatcgca atgaccccct atagcgcgct ggccagcggc cgcctctctc
    15181 gtaaagaagg ctatacacgg cgagccacag aggatgacta tgcgcgtgga aaatatgaca
    15241 gcacggctga acaggatcgc agcattattg agcgtgtagc cgaactggcc gaacgccatc
    15301 tggtgtcaat gacggaaata tctctcgctt ggctgctgac aaaagtcaca tcacctgtcg
    15361 taggggctac aaaaaaagct catgtcgatg gcgcggtaaa cgcagtaaat cttcaactaa
    15421 gtccggagga aattcagttt ctggaagaaa cctaccagcc gcatgttctg acgggggtca
    15481 tggcgcaaaa tacgccgcaa acgaaagatg ttaagcaggt ctggatgcga tagcccttat
    15541 tcacttttcc tcctctcgtc tttgagagcg ccagtagcgg tcggttacgt tcttaccagc
    15601 accagttgat cgaacgtctt aaacagtaac gtcagtaaaa agatcatcgt gaaaaagact
    15661 tttatgcttt taatttgcat gcaaataagc agccctgtct ttgcaacaaa accggacgcg
    15721 ccgaataagc cggagatgaa tatcttcgag ctcggcatca gacccgatcg ggacaacgac
    15781 tttgcagacg tggccagaca gacgatttcc gcttcggttg atcatgaagc aggtacactg
    15841 gcgatgtatg ccctgcaacg cagcgacaat tcaggtcagg cattcatgtt cgaactctat
    15901 gaaaatgagc gtgcttatcg caaacatctc aatgccgccc catacaaagc attcgctgct
    15961 cgtgcacctg acattattga caggaagaag aaaattaccc tggagccaca gtttctgggc
    16021 gacaaacaca tcataccgaa tgaacgaact atcaataatc tggtgatcgt cgaagtaaag
    16081 cctgaatttc agagcgaatt tagaaacatc gtccttcctg aaatggtcga gtcgctcaaa
    16141 gtagagaaag gcgtgttagc catgtatgcc ggtacagatt cagagactcc aaaacgctgg
    16201 tatttctatg agatttacgc cagtgaggag gattatcaac tgcaccgaca aacgctgcac
    16261 ttcctggatt acctcaggca aacggcgcat atgagcgcca ggaaggacgc aatcccggta
    16321 aaaccggtat ttcttcgtaa caaaggcgga atcaaacagg agccgcaacg ttgaccagta
    16381 aggattaaca tgaaaagcgt acttgcagca gcggctatgt cgcttgttat ttctgatttt
    16441 gctaatgctg aggaaacaag aggtaaagct atgatgaaaa tagaaccgtc agcgatttcg
    16501 gaagctgata ttcgttccgt ctcaccggct ctcgcgcgct ttggtcgcga agctattact
    16561 gaagatctgt ggacacgcga cgccctctca ccgagggatc gtagtgtggt gaccgtcgcc
    16621 atgctgattg cccgcaacca gcctgccgag cttaagcact atattgacgt cgctctcgat
    16681 agcggcgtga cgccagcgga attatcagaa atcattaccc atctggcgtt ctactcaggt
    16741 tggccaaatg caatgtcagc cgtcagcgtg accaaagcag tatttgaaac tcgtggcgtc
    16801 acgccagatg cgcttcctga cgcttctccg gacctgcttc ctctcaatca ggaagcagaa
    16861 aagcagcgtt cagagacagt ggaaaaaaat gttggcccaa tatctccagg cctggttaaa
    16921 ttcactgctg atccactctt cctggatcta tggcaaagac ccgcgctgaa accccgcgac
    16981 agaagcctga ttacggtcag cgccttaatc gcatccggac agagcgcgca gattggctat
    17041 cacctcaata gagcaatgga taatggactt tctgttgagg aagctggtga gatcgttacc
    17101 caggccgcct tctatgctgg ctggccaaac gcatttaccg ctgctccggt tgtgggagag
    17161 gtgctaaaca atcgatcatc gagtaaaagg taacattacc gaacactaaa acctgacgca
    17221 catcacaaca ttagaacgaa cagttcatga accaataagc gtaacatcga gtgatttatg
    17281 gacagcgttt aacacagtta tgaaccaaaa ctgagttcat aactgtgagt ttgaatcgcc
    17341 atttgattaa tatccattta aaacagtttg ttatgctgtt tctggtgctg aagtcaatta
    17401 tgtatatccc tcaaacccag aatagttaaa tggaatttga gtgatgcgaa gcgattttcc
    17461 agtcgtcgtt attacttcag ggttccagaa cataaagcct ctcctctttc atgattggcc
    17521 tgtgcatacc ggatataggc tggctggtat gcaggtttgc cgtttgccgc atcttccctg
    17581 cgttgaagag tcaggctggc aaaccgcgtg caaatgaaca cccccacttt ccatcaacac
    17641 ttcagacagc ttgcgggaat gagtccactc cggtatcaga aatggctacg cctcaatgaa
    17701 gccaggcgac tgatgctgaa tgaacattac gatgtcacca ctgcagccta tgctgtcggt
    17761 tacgaaagct tatcccattt cagtcgggaa tattcgcgga tgtttggaga gtcacctaag
    17821 agagatatta ccgggttaag aaagtctgtc ggtcagcttt aataaaccct gcacttatct
    17881 gtttagagca attccgtgtt agttacacca gtcaaacatt aaccatagct aatattttat
    17941 agccatataa accattggcc gctcttgacc tttcctgaca cttcccttgt tcgcaataat
    18001 attctgttta gtcgctgttt ataaataaca tgcataatga aatcgtaatg ttatttagcg
    18061 acataaaatg tctttgcaaa taccctctac aaca
//
LOCUS       DAOTRK010000096        17985 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.096, whole genome shotgun sequence.
ACCESSION   DAOTRK010000096 DAOTRK010000000
VERSION     DAOTRK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.096"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..257)
                     /locus_tag="QIE41_003114"
     CDS             complement(<1..257)
                     /locus_tag="QIE41_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDS3531972.1"
                     /translation="MQDIQQETLNECIRAEQSASVVLWEIDLTEVGGDRYFFCNEQNE
                     KGESVTWQSRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHG"
     gene            complement(257..595)
                     /locus_tag="QIE41_003115"
     CDS             complement(257..595)
                     /locus_tag="QIE41_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3531973.1"
                     /translation="MANTEEFHWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFKVEANERDSFEKFLARHEGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            complement(588..3821)
                     /locus_tag="QIE41_003116"
     CDS             complement(588..3821)
                     /locus_tag="QIE41_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDS3531974.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLTSSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKKFFDKGLIDKEEYEFLVRKLN
                     ETTEELSGVAQAQREAEKAGKLAAAQQEAQAQAFQRMLDKIDPLAAALRNLEQQQDEL
                     NAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQVAALQRLIAQLDP
                     VGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLRAMRSELEVTQSQLSKTGMSAK
                     QTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGPAIKGVGSYVLGL
                     INPFTLAAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQLADIAQRAGNAAD
                     STTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLVNDFNEIAKDPVS
                     AISKLNDQYHFLTIATYNQIKALQDEGNQQEAARIATEAYSSSMIQRTNQIKENLGYL
                     ETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTRPGVFGLGGIGDG
                     GAQNKRLARLKQQLGVLQAEKIAQDVLNSSINDYNKRQQEGIELRQRADAFSKQYQTR
                     EQQRASELAKLEKLKSQYSKEEYNNFIAQINERYKDPKQPKAKGYSDDAAQRMIDHLN
                     QQNALLSSQTELTVKLSSSEQELVKWRQQIADLESRPSSKLTQDQKSLLLHREEITAL
                     MEKNVAIEKNNRLIKESAEIAAWRDLLQASIDNRQQGYDIQIAGYGVGDKNQQRQQEL
                     LRIEREYNNQRLQLERDYADKSRGMSNHVFQEKMQALNDALEREKEIVRQKNEQLDIQ
                     AGDWVSGASQGFNNWLDDTKDISEQIKSTTTQMFDGMTDALGDFVTTGKANFRSFATS
                     VISDLSRIALKASITGIFDSISNSSSGGILGTIGSAISKFIPNAKGGVYESPSLNTYS
                     NGIYDSPQFFAFAKGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSKSGNGGGDITYAP
                     VYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGNLLNMRRDGGMLSG"
     gene            complement(3985..4185)
                     /locus_tag="QIE41_003117"
     CDS             complement(3985..4185)
                     /locus_tag="QIE41_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531975.1"
                     /translation="MLKELLYAYSVISRARRYAGMTGVPLPLSLTEINEYLATHPVLI
                     ERDEFEAVIFALDDQYFQDQCV"
     gene            complement(4293..4652)
                     /locus_tag="QIE41_003118"
     CDS             complement(4293..4652)
                     /locus_tag="QIE41_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531976.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDISKVSEVD
                     SVDDLLIDNAARYLLKDWKGVGELVNGAEVALEYTPERGIALLKQNPELYWQILAEAA
                     SIAQGKEQQKQDTIKKP"
     gene            complement(4803..5765)
                     /locus_tag="QIE41_003119"
     CDS             complement(4803..5765)
                     /locus_tag="QIE41_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531977.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKGE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLGWETKADDTNFIKGK
                     PVDSAGKLRYSFKEVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNTDIIQVQLSITAADEAPTITRKKCSPTAVIAKASADAIS"
     gene            complement(5792..6184)
                     /locus_tag="QIE41_003120"
     CDS             complement(5792..6184)
                     /locus_tag="QIE41_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HDS3531978.1"
                     /translation="MKFTEIRNTVISRMTAQTIIDGKDVLYPNGPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPVVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQAVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            complement(6181..6561)
                     /locus_tag="QIE41_003121"
     CDS             complement(6181..6561)
                     /locus_tag="QIE41_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDS3531979.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAMQILTAIDIAAPVDTG
                     RFRNNNLVSLQHPDFGISDNVDPNGTIAVQRGIGVISKAANYGVIYIQNNLPYAEALE
                     NGHSQQAPTGVYANAFYGVLQAYK"
     gene            complement(6562..6945)
                     /locus_tag="QIE41_003122"
     CDS             complement(6562..6945)
                     /locus_tag="QIE41_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDS3531980.1"
                     /translation="MGLREEIQSEVAAAFDEDLADAVSDFSGYYVTHRHWDPVTETGG
                     ESTAIYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVDHIIEIYDPVSRQ
                     LQRYEVITASLDPSASVYSIQLRRA"
     gene            complement(6945..7340)
                     /locus_tag="QIE41_003123"
     CDS             complement(6945..7340)
                     /locus_tag="QIE41_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HDS3531981.1"
                     /translation="MITYVTCDDVDNAFGNAWTSENAKNKAVLMANAWLNGFNLKINP
                     SRIPEEVKLAGAYAARIASFGKLFQQKNDSGVVISKAVSADGVSVSKSFSELPANSTA
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            complement(7344..7520)
                     /locus_tag="QIE41_003124"
     CDS             complement(7344..7520)
                     /locus_tag="QIE41_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531982.1"
                     /translation="MAGLTKEQRAQREAAQKTQIELVVMVTDYQMFPGAPTIANVHPD
                     EVDNWKAMGWKTQE"
     gene            complement(7563..8702)
                     /locus_tag="QIE41_003125"
     CDS             complement(7563..8702)
                     /locus_tag="QIE41_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HDS3531983.1"
                     /translation="MAGNTLTGLIPTIYTALDVVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTAPVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGAPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGSISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVVGAFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            complement(8801..9565)
                     /locus_tag="QIE41_003126"
     CDS             complement(8801..9565)
                     /locus_tag="QIE41_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531984.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLATKKIT
                     ELNSEAKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLLDAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLEKQLYDSMIGGSFAGSKYIADKIAIPADLLQSRFGQAF
                     KVEEGRIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDIGQKTMKRSAFDALDVAGKQNALKDGITIVD"
     gene            complement(9670..10782)
                     /locus_tag="QIE41_003127"
     CDS             complement(9670..10782)
                     /locus_tag="QIE41_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531985.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKILNDSDAELTAR
                     LLIALDSLDPGSFTVKRLESLLASVREVNRTAINSMFTSLSGELNELAIYEAGYQLSL
                     FDSMLPDFVADVHPLVGISPDALYAAAMARPFQGRLLSEWASDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLNNKPVGHSVPYLQGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKQIVGETRAKLI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQVFRKAGI"
     gene            complement(10766..12172)
                     /locus_tag="QIE41_003128"
     CDS             complement(10766..12172)
                     /locus_tag="QIE41_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDS3531986.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPAKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMKEIRTKADENAAGSRPYVTMLNPRQVIGW
                     KSKVEKGKVVLTDLRIKEVIIIDGDDFGQKKVEQIRHIMPRRVEIYRRSEGTNGESVW
                     TLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVARV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQENSPLYTMANSLEDALDNILQIMAEWIGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAIRVLQALKFIDQDSRPEEVID
                     ELKNQSVMLMEINDANRE"
     gene            complement(12175..13476)
                     /locus_tag="QIE41_003129"
     CDS             complement(12175..13476)
                     /locus_tag="QIE41_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDS3531987.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPGINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLRGQFTNLTSGTVYHQFDRKLNNCEEVEQPGEPIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRIIKERFWLYDGNDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPLYAESLEQQVW
                     DEKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            complement(13457..14551)
                     /locus_tag="QIE41_003130"
     CDS             complement(13457..14551)
                     /locus_tag="QIE41_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDS3531988.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     TPKNAQTEMCKTAQKSAQKKSAQTAQKRNEKSQEKKPVFDAGLNEGDAEEFSFCPDEF
                     GISDQQAKFAMLVAQGKKPTEAYRLAGYEGQGATANSNASRMLRNARVFRAISYFRNQ
                     YQKRYTADLDLLVSQLMAIVLADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGAK
                     FGKFGVEILLEDKAAARRELLRLLSAGGDLCADKRLQELEIERRRMENQKLRKEIETV
                     EDNEHPQPVAININVVDARVRSDEDDLSDA"
     gene            complement(14555..14806)
                     /locus_tag="QIE41_003131"
     CDS             complement(14555..14806)
                     /locus_tag="QIE41_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531989.1"
                     /translation="MIPYSKVESLAACRMTAQQIADVLDVDLNRLKENREAMTDFYAA
                     IRKGRAKGEAELRAALFKLARKGDAFALRELLRVDKNQD"
     gene            complement(14742..15674)
                     /locus_tag="QIE41_003132"
     CDS             complement(14742..15674)
                     /locus_tag="QIE41_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3531990.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     NQYMAEFGEQVLVFSKADIASRFDEADNFCDRRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNLGRCGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYAPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            complement(15667..16458)
                     /locus_tag="QIE41_003133"
     CDS             complement(15667..16458)
                     /locus_tag="QIE41_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDS3531991.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSPEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFIPTEIDELLTDVLPGTGNEEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLEPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     SEIQCFFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEYA"
     gene            complement(16596..>17985)
                     /gene="trkG"
                     /locus_tag="QIE41_003134"
     CDS             complement(16596..>17985)
                     /gene="trkG"
                     /locus_tag="QIE41_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HDS3531992.1"
                     /translation="MLPPMVVALFYKEKSLFVFFITFVIFFCIGGGAWYTTKKSGIQL
                     RTRDGFIIIVMFWILFSVISAFPLWIDSELNLTFIDALFEGVSGITTTGATVIDDVSS
                     LPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGGAKLYQSEMPGPFKDDKLTPRLAD
                     TSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGISTVSLGGFSTHSESIGYFNNYLV
                     ELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIELRFFLLIALGVIIVTSFQVWHIG
                     MYDLHGSFIHSFFLASSMLTDNGLATQDYASWPTHTIVFLLLSSFFGGCIGSTCGGIK
                     SLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVTDRVMRSVWSFFFLYTLFTVFFIL
                     VLNGMGYDFLTSFATVAACINNMGLGFGATASSFGVLNDIAKCLMCIAMILGRLEIYP
                     VIILFSGFFWRS"
BASE COUNT         4658 a         4739 c         3637 g         4951 t
ORIGIN      
        1 ccgtgcagat tagagacttt cagcgttggc cttgcactgg cacctttgcc gttcatctca
       61 aatccactcc cctgaatggg ataggcctga tactgccgac tctgccaggt gacagactct
      121 cctttctcgt tctgctcatt gcagaaaaag tagcgatccc cgcctacctc ggtcaggtcg
      181 atttcccaga gcaccacgct ggccgactgc tccgcacgaa tgcattcatt cagcgtttcc
      241 tgttgtatgt cctgcatcag atcaccactt cgtcaaactg acacgaaaaa tcggtatagg
      301 tgatatgttc tgtcgctgac cacgttcgac acaccactct gatttttctg ttaataccag
      361 gcgggcgcca aaaaaaagat ttatagcctt catgccgggc taaaaatttt tcaaatgaat
      421 cacgttcatt cgcctcaact ttgaaatcac aggtgaaaac gcgcagagaa tgattaagtc
      481 catttggact tcgttgctca taaccatcgc caaatcttac agtttttatc gatggtttat
      541 tttctgtttt cattccatcc tctggtaacc agtgaaattc ttccgtatta gccacttaac
      601 attcccccat cacgtcgcat atttaacaga ttgccctgca cccgctgatc aaccatcccc
      661 ataagtgctt ttattgcctg agggccaatc tctccattct ggccgtcatt ttgaatagtg
      721 atttggtata caggggcata ggtaatatct ccgcctccat taccactttt actattaata
      781 gctctgacac caagagaacc atcggatgtt cgtgttaatg gcataatggc ttccggacca
      841 gcctcaccaa aaacaccagc cccttttgca aaagcaaaaa attgcgggga atcataaata
      901 ccgttcgaat acgtgttcaa tgacggagac tcataaacac cgccctttgc attcggaata
      961 aatttactaa tagcactccc gatagttcct aaaatccccc ccgaagaact gttactaatg
     1021 ctgtcgaaaa tcccagtaat tgaagccttt aatgctattc gactaagatc cgaaatcacg
     1081 gaagtagcga aagaacgaaa atttgccttg cctgtcgtga caaaatcacc cagcgcatcg
     1141 gtcattccat caaacatctg agtcgtggtt gattttatct gctcactgat atccttagtg
     1201 tcatccagcc agttattgaa tccctgggag gcaccactaa cccagtctcc tgcctgaata
     1261 tcgagctgct cgtttttctg tctgacaatt tctttttctc gttccagagc atcattcaga
     1321 gcctgcattt tctcctgaaa aacatgattt gacatcccac gggatttatc tgcatagtca
     1381 cgttcaagtt gcagacgctg attgttatat tcacgttcaa tccgcagtaa ttcctgctgg
     1441 cgttgctgat ttttatcgcc aaccccataa ccagcaatct gaatatcata cccctgttga
     1501 cgattatcaa tcgaagcctg caataaatca cgccatgcag ctatttcggc ggattccttg
     1561 attagcctgt tatttttttc aatcgcaaca tttttctcca tcaacgcggt tatttcttcc
     1621 cggtgtaaga gaagcgattt ctgatcctgg gttaatttcg atgacggtcg tgactccagg
     1681 tcggcaatct gctgacgcca cttaaccagt tcctgttcag aggaacttaa tttaacggtt
     1741 aattcagttt gtgaacttag taacgcattc tgttgattca gatgatcaat cattcgttgg
     1801 gcagcatcat ccgaataacc tttcgccttt ggttgctttg gatctttata acgctcattt
     1861 atttgagcga taaaattatt atattcttcc tttgaatact gactctttag cttttccagt
     1921 tttgcaagtt cactagctct ctgctgctcc cgggtctgat attgttttga aaaggcatct
     1981 gctctctgtc tgagttcaat tccttcctgt tgtcgcttgt tgtaatcgtt tattgatgaa
     2041 tttaggacgt cctgagcaat tttttctgct tgaagtacgc ccaattgctg ctttaatcgt
     2101 gccagccttt tattttgagc tccgccatct ccaatgccac ctaacccgaa aactccgggt
     2161 cgggtatttt tttctatttc atcaatttga cggagaacat ctgagatttt ttgatcaagg
     2221 gaggcctcac ggccaatgtc cagcatggaa tcccatgccc attttgcgga gtctgcgaca
     2281 gctttccatg cagtctcaag ataaccaaga ttttctttaa tctggttggt gcgctggatc
     2341 attgaggatg agtatgcttc tgtcgcaatg cgggcggcct cctgctggtt cccttcatcc
     2401 tgtagcgcct taatctggtt ataagtcgca attgtcagaa aatggtactg atcattaagt
     2461 tttgatatgg cactgacagg atcctttgca atttcattga agtcattaac cagttgttcg
     2521 gttgatattc ctgttacttc gctcgttttt actatcgctg tcgtcacttg ctccagcgaa
     2581 ctgctcgcta cctttcccga acgcacaagc tggtttaata ctgccgccgc agcgccagtt
     2641 gtcgaatcag ccgcattccc ggcacgttga gctatatcgg ccaattgccc gctggttgtc
     2701 cccaactgat ttccggtaag aataagagat ttattaaatt cgtcctgctc ctgagagcct
     2761 ttatagtaag ccagccctaa gacgccaacg gctgctgcgg ccagggtaaa aggattaatt
     2821 aatcccagca cataagaacc aacacctttg atcgccgggc caatcccacc gaacatatct
     2881 tttagctggc cgccctgctg cattaacacc ataaaaggcg actgaccagt ggacaaccca
     2941 acaacaatat ccgtcatttg tgcaggcaac atgcgcatag caaaagccgt ttgttttgcc
     3001 gacattccgg ttttgcttaa ttgagattga gtaacctcaa gctcactccg catagcacga
     3061 agttttccag aaagttcctc atacatttca ggagagagca tccccttagc ttttgcttca
     3121 ttgagctgtt tctgttgttc taccagacga ttaaaagcag ttccgacagg gtcaagttga
     3181 gcaatcagac gttgcaaagc agcaacctgt tcatcatgcg cttttgctgc ttctcgctct
     3241 gcctgagcct ctccggtaag ctctcgccgt gtttcctgta tttttcggct ataattctca
     3301 aactgagaac catttatttt cccggatgca aacgcagcat taagttcatc ctgctgttgt
     3361 tcaagatttc ttagcgccgc agccagaggg tcgatcttgt ccagcattct ttgaaaggcc
     3421 tgagcctgcg cttcctgctg ggcggcagca agttttccgg ccttctcggc ttctctctgc
     3481 gcttgcgcaa ccccgctcaa ttcctctgtg gtttcattaa gtttacggac aagaaattca
     3541 tattcttctt tatcaataag ccctttatcg aaaaatttct ttaattcaga atagcgtcga
     3601 ccgacagtat caattgcggc accaactgga tcaatagctg cttttaattt tgcgagcgcg
     3661 ttcttctcat cttctgttgc cttagtcact ttccctgcgc tatttgcagc agtttccccg
     3721 gcctgcgtca ttttgactaa tgaggaggtc agattgtcag cattattttt tgctccagtg
     3781 ctatcaataa ttattgcgag acgcgaggtt tgctctgcca tttattaaaa ctccagacaa
     3841 caaaaagccc accgaagtgg gtttcaggcg acataatagt agatatagcg attacgaggc
     3901 cacgcaatgc ttttctccag gagcatcatc gatttaatta aagacaccat cacatctctg
     3961 taacagagtg tacgtaatta acaactacac acactgatcc tgaaaatact ggtcatccag
     4021 tgcaaagatc actgcttcaa actcatcgcg ctcaatcaat accggatgag tggctaaata
     4081 ttcatttatc tctgtcagag ataaaggcaa aggcaccccg gtcattccag cataacgtcg
     4141 ggcacgggat attaccgaat aggcgtacaa caattcctta agcaccgggt ctatttctgg
     4201 ttccggtatc ggtggcaacc tgagtttttc tcgcttccat cttgcctttt cccccctttc
     4261 tcccccgaac tccgataacc accgctgggc ggctatggct tttttatcgt atcctgcttc
     4321 tgctgctctt taccctgggc gatgctggct gcttctgcaa ggatctgcca gtacaactct
     4381 ggattctgct taagcagcgc gatccctcgt tctggcgtat attccagtgc aacctcagca
     4441 ccattaacca gttcaccaac ccctttccag tctttcagca gataacgagc agcattatca
     4501 atgagtaaat catcaacaga atctacctcg gaaacctttg aaatatcaaa ctccttcgtt
     4561 ccgacgtgca aactggcatc cattttctca atgtgacgac ggattaatgc attacgggag
     4621 cgatactgat cgttatcgct gcttgccacc aacagtttta acccgtctac aggttttaag
     4681 tccttcattg gcgtaaacca gcgttctcca ccgatgataa ttttctgatt aagaataaac
     4741 atccataacc tcattcaatg cgtccccctg tagtgcagta ccacaggggg aataacggaa
     4801 aatcaactaa tcgcatcagc actggctttt gcgatcacgg cagtcgggga gcattttttt
     4861 ctggttatcg taggtgcttc atctgctgcg gtaatgctca attgaacctg gataatgtca
     4921 gtattacccc catcaggcca ttcacctgac acctgaacct tcgggaaact gaaagtgtat
     4981 gccccctctc cattcgaaag cgtgaagctg aacggaactg tttctccagt cagtgtttta
     5041 ctccagattt cccacgcggc tttagaccat gaaagcgtca ccgtaccgga cggtgtaaaa
     5101 gtagtcggaa tatttgctcc tgcataaggc gagccagtcc cgatacaacg ctgtgtctgg
     5161 agtttgttat cgaactgaat atcaaaactg tcgatacaaa aaccgttacc tccggcaaca
     5221 ccattcaggc ttactgctga aacctcctta aacgaatagc gtaactttcc tgcactatcc
     5281 acaggtttgc ctttgataaa attcgtatca tcggcctttg tttcccagcc cagcccggca
     5341 aaggttacgg tcgctgtaat atcgccgtcg ttagggatct gcattttcca tgagccaacc
     5401 tgcgctcctc tgacgacaga ggcaattccg acatcggacg cataggtcgc cagagaaaat
     5461 gttattcgct cattccccat tgtcagagaa tcgcctgacc attccgcgcc gaaacaggat
     5521 gcaagaaaat catcatgttg accccagcga aatttggtac caacatcacc gccgacatcc
     5581 acagtgccag gcgtcgcccc ctgagccatc cgggagccac caatctcatt attttcgcct
     5641 ttattctggg tgggttttac tccccagctt gtgcgtttta ataaactcca gtcaccactt
     5701 gctggcgtag tgcctgcaac tgtctcccgg ataaatgccg agataacctt tgcacctgaa
     5761 ctcacagaag cctcctatgt cattaattgc gctagagcgc gcgatatgga atttgaagat
     5821 taagctggaa ccagccattc ttttcgccga cggctattga ggaaacagcc tggtaactga
     5881 gacgatcgtc atcctgaaac tcaaacagtt cccgcaattt atcggctgtc tcagtaatga
     5941 gttttgaacc agaacctgcg gggacaaata actgaataat gattatcccc gtgcgataaa
     6001 caaccggccc cgcgccaatt tcattaaccc cagcctgccc gggaatatta cttaaccgcg
     6061 cccagattaa cttaccggaa ggatcgaacg ttggcccgtt cggatacaat acgtcttttc
     6121 catcaataat cgtctgtgcc gtcattctgg aaataaccgt atttctgatt tcagtaaact
     6181 tcatttgtaa gcctgtaaaa caccataaaa agcgttggca tacacgccag ttggcgcttg
     6241 ttgtgaatga ccgttttcaa gagcctctgc ataaggaagg ttattctgga tataaataac
     6301 cccgtaattt gcagctttcg aaataacccc gatcccacgc tgaacagcaa tcgtaccgtt
     6361 cggatccacg ttatcagata taccaaaatc gggatgctgt aacgacacca ggttgttatt
     6421 tctgaaacgt ccggtatcaa ccggagcagc aatatcaata gcagtaagaa tctgcatagc
     6481 gatgtagcga atcttcagcc ctacatcttc ctcaatcatc ccggcaaata ttgacggttc
     6541 tatatcccat gcctttgcca tttacgctct ccttaactgg atagagtaaa ctgatgcgga
     6601 aggatctaaa cttgctgtaa ttacctcgta tcgttgtaac tgccttgata caggatcata
     6661 aatctcaata atatggtcga cagctggttt atccgtaacc tcgcatacca gagcggttaa
     6721 tttaaggtca ccatgcaaaa tattaatccc atcaattctg cccagcttat aacgtgtcaa
     6781 cacgcctcgc ccggtataga ttgcggtgga ttcgccgcca gtttccgtca caggatccca
     6841 gtgccggtgc gtaacgtaat aaccagaaaa atcactcacg gcgtccgcta aatcctcatc
     6901 aaaagcagcg gcaacctctg actgaatctc ttcacgaagc cccattatcc ccccctcaca
     6961 accctgactt gtgagcgact aagtccgtac ggtttcagca gtgctatcgc aagctgtaaa
     7021 tcgggttcaa gcaatgcagt gctgtttgct ggcaactcag aaaatgattt cgatacactg
     7081 accccgtcag ccgacacggc tttactgata acaacgccag aatcattttt ctgctgaaac
     7141 aacttaccaa atgaggcaat tctggctgca tatgctcccg caagttttac ctcttccgga
     7201 atacgggatg ggttaatttt caggttgaag ccattaagcc aggcattagc cattaaaaca
     7261 gctttatttt tagcgttctc actcgtccag gcgttcccaa acgcattatc aacgtcatca
     7321 caggtcacgt aagtgatcat gtgttactcc tgagttttcc agcccatagc cttccagttg
     7381 tcaacttcat caggatgaac attagcgata gttggtgcgc cgggaaacat ctgataatcg
     7441 gtcaccataa ccactaactc aatttgcgtt ttttgtgcgg cttcacgctg tgctctttgc
     7501 tctttagtta atccggccat atgcccccca ttaaaaatgg ggccgaagcc ccgtttgaat
     7561 gtttaaccaa gaatcagaca accatgtgcc ggcttcactg atgaaacacc ccatgccagt
     7621 ccaacttcat agcgcacctg gcgatactga cgatacagtg caatctggaa cgtaatccct
     7681 gaaatcgggt ccgtaacatt cattacatca tcagcattat cgccaccttc cggcattgcc
     7741 ggagtacggg atgccagcag aaacgcgttg cgatcaaacg ccatatttgc agtaaaggcc
     7801 ccaacaactg tgatggcagt atcatcggcc agatcctgac gcagtccggg cgctgcaaga
     7861 gtaatcagat tgctggtcgc tgctgccaca acatactgat tcggatcacc agcgaacgta
     7921 acaatctgac ctgcagaaat actccccgaa ccagtatcaa tggaaataag aacatcgcct
     7981 tcttttttct ctccattcac gagataacca gttgcagcaa cctttggcgc tcgtttcaca
     8041 cctgccgaac tgtgaatatt gaatccctcc agacgcccca acacgccctc acgcagtagc
     8101 tgttcagtgc cggattcatt cactttaaac aatacagact gttttccgcg caaattagca
     8161 atggcagtgg agccaagcac catctgcaga tcggttgtcg gtgcgccgtt atcctccaga
     8221 acctgacgag ccagagcagc atcagaaaga tcatctttaa caccaaacgg cgttgtccct
     8281 gcggttccca cggcgcggga agcaccgaaa tacaacgcac caagatcagc ctcaacctcg
     8341 tttgcaaggg cgcgaaaagc ctgcttgaac tgatcagcca gaatggtgtt gtaagtccct
     8401 gaaggaccaa gagccagttg ttcttcacca ttccatttaa ccggtgccat tttggattta
     8461 gtaattttta catcaacagt gccaatattt tgatcaccgg tattcggagc tgacggcccc
     8521 ggtacgatat cttcggtttt cgcctcaggc gcaactggcg cggttaccgt ctgatctttt
     8581 gctgcggcgt cagcttttgc gttttttgct accgcaggga taaaacctac ctgctcacgg
     8641 gatacaacat ccagggcggt ataaatagtc gggatcaacc cggtcagggt atttccagcc
     8701 ataattaaat attccttaaa aatttgcgta attgtgaata gatggagtaa tgagctatcc
     8761 agccctgaca ccagctccca tccggaagct gacaaatgtg ttaatcaacg attgtgatac
     8821 cgtctttcaa tgcgttttgc ttacctgcaa catccagtgc atcaaaagca gagcgtttca
     8881 tcgttttctg accaatatca tgctgtgtcg gacgggagcc gccgccattg ttgccactgg
     8941 ctttcaggat gtagtctttc tgagggtaat tttcgacaag gaactccagc gcctcatcaa
     9001 actgcgccag ttcgccaggc ttcgcgcggg aataaatttt gttgccggaa gcgtcataag
     9061 caacgatcct cccttcttcc actttgaatg cctgcccgaa gcgtgattgt aataaatctg
     9121 ccgggatcgc aattttatcg gcaatatatt ttgaacccgc aaaactaccg ccaatcatag
     9181 aatcgtaaag ctgcttctcc agcatctgag agcgttgctt ttcttcatct aattgctgct
     9241 gaaaattttt cgtaatttct gccttaacct ggtcaacctg tcccgcatcg agcagctttt
     9301 tctggtcgat ttttgacagc atttccagtg cctcgatcgc cttcttcggg tcttcgatag
     9361 cggcaaactt agccagtttt tcctctgcag cttctttagc caggcgatga ttttttgcct
     9421 cgctattaag ctctgtaatt ttttttgtcg ccagcggtgc atcgaagcca atttctttac
     9481 catcgtcgtg cacataaact ggcaggccag cagtatcaat ttctgcgtat tgttttccgt
     9541 taatctcgac cgttttcagt ttcatattag tacctggttt aagtcttccg acagttacgc
     9601 tgctcaccat ccggatcgca gcaataaaaa aggccacccg aaggtagcct gttgtaataa
     9661 atgatttatt taaatccctg cttttctgaa tacctgtgca tcacgctcac ggagctgctt
     9721 cagcgtcagc cattcgcctt tatcggtgta aaattcatct ggcgacatac caccatcccg
     9781 aatcagcttt gctcgggttt cccccacaat ctgtttttgt ctggtgtaag gctgacgcaa
     9841 aaaccattcc ctgtaggttg tatctccggc caccacgcca tccatgctgg cccgctcagc
     9901 cggggaaata tcgcgaacat caatacccag ttccttcgct gatttcagaa tgaacgtttc
     9961 cgttgagcgg cagcagaaat gaatttttcc cggcccctgc aaataaggca cgctgtgacc
    10021 tacaggttta ttattcagcg tatatttgag gcgatccctg attcgacatt gtggcgtagt
    10081 acgattatca agcgttgata accattgctt acccttaatc aaatcattat tcgcgctggc
    10141 aaaactctca cgggcagtag cagcaagatg tccaaccgct gtttttgcaa tgctggccgc
    10201 attagcccgg ctcatctgca atgcaccatc ctgaaatccc ttactgacat gtccccgaat
    10261 ttttcttgcg atctgctcat tggtatcccc cagcaaaaaa ccctgacgca ctgtatttgt
    10321 tatgcgtctg agccgatccg cctcaagatc tgaggcccac tcactgagca gtcgtccctg
    10381 aaatggtcgc gccattgcag cggcataaag tgcatcagga gagataccaa ccagagggtg
    10441 aacatcagca acaaaatcag gtagcataga atcaaacaga cttaactgat aaccagcctc
    10501 ataaattgcc agctcgttca gctctccgga gagactggta aacatgctgt taatagcagt
    10561 gcggttaacc tctctgacgc tcgccagaag tgactccagg cgcttaacgg taaaactacc
    10621 tggatcgagg ctgtccagtg ctatcagcag gcgagcagta agttccgcat cgctgtcatt
    10681 cagtattttc accattctgg cagccacgcc agtgctatag cgggatatcc agactgcatg
    10741 agcaattgat tcatcacgca gccgttcatt cacggtttgc atcattgatt tccatcagca
    10801 ttacactctg attttttaat tcatcgatca cttcctctgg acgggaatcc tgatcgataa
    10861 atttcaacgc ctgcaacacc cgaatcgcat caatctgacg tatatcaccg ccctgacgca
    10921 atgactgaac agccagcgcg gaggatgagt caaacacctg ggcagataca tccagttcag
    10981 tgcgcacatc cacattgcca ccgcaactct ctccgatcca ttccgccatt atctggagaa
    11041 tattatcgag ggcatcttcg agggagttcg ccattgtata aagcggcgag ttttcctgca
    11101 ttcgctcttc attagtctga tcaacagatt tggtggatgt attttcagca cgcagaagtt
    11161 tagcgccggc atgacgcatc tgattttcca gcttctcaag tgatgtttcg ccagattcta
    11221 tcgctgcgcc actatgttca acatattcga ggccattttt tgttctgtcc tcaaaaatcg
    11281 tagcggtgga tgcaccaacc gtcagttctt cattcctgtc cagcccgtag gccaccagca
    11341 atggaacgcg ggcaacatga agaatattgt cctgctcgct ctggctttgc cagtgcttga
    11401 tattcagcaa gccaagatta agcaatggcg gtgtaccacg cataaatcct gttttcttcg
    11461 tgtacagtgt taccagagga atatcatcac ggctggtatt ccatgactca tgaagcgtcc
    11521 agacagattc gccattagtg ccttcgctac gtcgataaat ttcaactcga cggggcataa
    11581 tatggcggat ctgctccacc ttcttctgcc cgaaatcatc accatcaata atgatgacct
    11641 cttttatacg caaatcagtg agaacaactt tccctttttc aactttcgat ttccatccaa
    11701 taacctggcg tggattcagc atcgtaacgt acggacgact accggccgcg ttttcatcgg
    11761 cttttgtccg aatctctttc atatccgttc gtggatagtc caccagcgca tgtgccacac
    11821 catactgaaa tgcgaggctg aaaaattgct gcgcccacac atccagtcgg cttccctcca
    11881 tgtcgatatt ttctgcatat tccctgattt ttgccggcgt ttcctcactc aatactgtcg
    11941 gctctgcaaa tatgcgccca atattttgct tgatgctttc ttcatacaca ggaagtagcg
    12001 tagccacaga caggcgtttt ttataagcgt cttcatcttc gttaggccat tttgggagat
    12061 aattttcccc ctgcctgcgc atttcaagcg taccgcccat cagtgcgtcg ttaatatccc
    12121 acgcctccag catatcgtta tagtcgaggt tgggtgttga tatatcagcc ataattaaat
    12181 ccgaagtgat gtgactcttc cggtcggttt gacaataggg aattgcttaa cgatgaaata
    12241 acctccggca tcattcgggt gatcattgcc agatttttta tcaggctccc ccttctcatc
    12301 ccagacctgt tgctccagag attcggcata taacgggcaa cgcttcacat taactttata
    12361 gcgacgctca ccattggcat tgcagaacat tgcattcatt gagttaacgc gatcttttac
    12421 tggcgggttc gagctgttca ccacaacgtt aaaaccagcc tgcttaagct gggctatatc
    12481 cgtcgtactt gcgttacttg attttctgga atccccggaa gcatctggat aaatataaat
    12541 ttccctcact ttccggtaat cattcccatc atacagccag aagcgttctt taatgatgcg
    12601 gatcatatcc ggcgtatcgt aggcattgat gatttcagtt accgcacatg gaagccccaa
    12661 acgcagcaca tggacgattc ccgccatctt tccaacgtta aaatccatcc caatataaat
    12721 cggctcccct ggctgctcca cttcttcgca attattcagt ttccggtcaa actgatggta
    12781 aacagtacca cttgtcaggt tagtaaactg tcctcgcaga taggctttaa tcagctctgg
    12841 agggtatgat tcaagaagcg aaggaatgta atctgctggc aggttctttt cattatcgaa
    12901 agtagatgcc tgcaccagac catacagtga ggccagctct gttttttcac gcacggcttt
    12961 aacaaactgc tcgtagacaa atttgaatcc ttccggcgtg gttgtaacgt caataccgtt
    13021 gcgaagtcca tcaatcttat aacgcatacg cgcaattatc ttgcgccacg ccgttctggc
    13081 tttttccttc ggcaaaatgt ccagttcatc caccaacgca ttaccaattt tgaaaccgac
    13141 gatcgtttgt ggcttctcca tcgatctgca gatagtggtt ccccgatact ggcgtccgta
    13201 ataaaagtga acctctttat ttccctcatt aatttttacg ttcaatcccc agtcagcagc
    13261 aacttcttcc actgtagggt aaaaaatatc gcgaatttgg ggatacgttg gcgcaaaata
    13321 tccctgattt atacctggat gctcccaaat ccccttgcat atgccgccac acccaaccca
    13381 tgttttgccc gagccaaaac cagcaatata ggctttaaat ttatggggca ttgaaagaaa
    13441 tcgcgcctga ggcacattaa gcgtcggaga gatcatcttc atcactcctt actctggcat
    13501 caaccacatt aatattgatc gccacaggct ggggatgttc attatcttcc accgtttcga
    13561 tctctttgcg cagcttctgg ttttccattc tgcgccgttc aatttccagt tcctgtagcc
    13621 gcttatctgc acataaatcc ccgccagcag aaagcaaacg caacaattca cgccgggcgg
    13681 cagccttatc ctccagcagg atctcaacgc cgaattttcc gaattttgcc cctgcatata
    13741 attgccgcgc atccccatca agcagagtgg tatcagccat ataaagctgt cccgttccct
    13801 caccgcagca cttcgggcag tccggattgg gtatggcgtt atcaacaaag ccgaggcctc
    13861 catattccgg ttcgggtttg ccatctctgg aagcctgcgc cgctgccttg tcgaattctg
    13921 ctatatcgcg ccactggtag agatgattct cgccccagca ataacggcag ttaacacggc
    13981 gaaattgtgc aagctgattg gggtcggcca ggacaatagc catcaactga ctcactagta
    14041 aatccaggtc tgcggtatag cgtttctggt actgattgcg gaagtagctg atagcgcgaa
    14101 aaaccctggc atttctaagc atacgactgg cgttgctgtt agctgtcgca ccttgcccct
    14161 cataaccggc tagtcggtac gcctctgtcg gctttttacc ctgagcaaca agcatcgcaa
    14221 acttagcctg ctggtcagaa atgccgaatt catcgggaca gaacgaaaat tcctccgcgt
    14281 cgccctcatt caggcccgca tcgaatactg gctttttttc ctgagatttt tcgttccgct
    14341 tttgtgcagt ctgcgcagat tttttctgcg cacttttttg cgcagttttg cacatttctg
    14401 tctgcgcatt tttcggagtt tttttgatgt aacgacgggc tgttgcgtaa ttcagtcccc
    14461 ttgcttcaca ccatgccacc ggagatatac cggagcgggt gtattcagca atatactcct
    14521 gctgcaacgc cccccagtcc ggtctgctca tcagttagtc ctgattttta tccaccctga
    14581 gtagttcgcg cagagcaaag gcatcccctt ttctggcaag cttaaacaat gccgctcgta
    14641 actcggcttc acctttcgct ctgcccttac ggatggccgc ataaaaatct gtcattgctt
    14701 cccgattttc tttcagtcgg ttcagatcaa catccagaac gtcagcgatt tgttgtgcag
    14761 tcatccggca cgctgccaga gactcgactt tcgaatacgg aatcatttgt cacccccatt
    14821 gatatgcagg gtgtcttctt cctgtatttt tcgtgaagga tttttactgc agcgttgttc
    14881 caggtgacct gatggtgaat gcgtttatgg ctggcaccca tcagtgagat ttttacgcac
    14941 gacggcgcat acatgacgga gtaaaaactt ttaacgtagg ttccggaatc cagatacagc
    15001 tcggtcattc cgccgctgtt tttctgcgtc tgtttctgcc ctaactggac agcaccgatc
    15061 gtcataaaca attcaccaca gcgaccgaga ttcgtgtacg tattcacatc ctcgttaatg
    15121 cgccccatga atgagaacgg tcgatcaacc gaacagataa agctgttcat tgccttgcgt
    15181 ttcacccacg aagcatggcc gccattgtca ccaagaaaat ccccgccctg cgacatagcg
    15241 atggaaagag caggtattga ttcgtagtac gccagcattt cagaaaggat cgcatccagt
    15301 ttccttatcg gaaaataggc ctggtcatag ttgcgatcca cccgaaactg gaactcgtga
    15361 taatcatcat cgagctgaat gaagtattta cacccgacca gttttgccag gtcgaaacag
    15421 gcattacggg cgtaaaaaat tgagcggcgg tcacagaaat tatcggcttc gtcaaaacga
    15481 ctggcgatat cggctttgga aaacaccagc acctgttcac caaattcagc catgtactga
    15541 ttccgtgtct tatcttcatc atcaacaacg ataaaaattt tcccggtata gccagcacga
    15601 cgcaacgtcc ggtaagtcag aactttgtcc ggtcgcccgt gagtcagaat aaaggcgcaa
    15661 aaatcatcac gcatattcct cctcctcacc accatgcatg atctccacca tgcgctgcgt
    15721 catccggaca aatccatttt caatagcctg ctgataatca atgatcacca gcgccgactc
    15781 ctcgaaaaaa cactgaattt cagagggggc gtgagcgtaa tagtccgcaa ttctgctgaa
    15841 attaaacacc gtgtgacgtt ctgccgcaca caggaggaat ttctcaatat caggctcaag
    15901 ggacgccgaa cgtatccggc tgatcagctc ctgagttttc gtatcgtcgt acagttcact
    15961 gatatccggt ttaccgcccg acggctcata aacaggcgta tcaattttcg tcgtatacgg
    16021 ctcctcctca tttcctgtac cgggcaaaac atccgtcaac agttcatcaa tttctgtcgg
    16081 gatgaagcct gtcagggaga catcaaaatc agcattgatt aggtccgaca gctccatccg
    16141 caacagatct tcatcccagc cagcattcat cggcaggcga ttatctgcca ggcggtacgc
    16201 ctttttctgc tcatccgtca ggccagacag aacaatgacc ggaacagaat ccattttgag
    16261 catttcagcc gccataacac gaccgtgacc cgcaataatt tcgccctttt cgtcaatcag
    16321 caccggatta gtccagccga attgcttaat actttctacc agttgtgcca cctgctcagg
    16381 gctgtgcgtc ctggcgttgt gcgcatacgg tgacaattct tgtaatgggc gatagactat
    16441 ctttaatttc tcgctcatac agccttgctt tatgaataaa acgcacccca gcagccagtg
    16501 ctactgggga cggaggggtt gctggtaaag ttaggtattg gatcaatgag tgagtcaaca
    16561 taatattaaa ctcacaatta taaatcagcc atatattagg agcgccaaaa aaaacctgaa
    16621 aacaatataa taacaggata aatttcaagg cgaccaagaa tcatagctat gcacattaaa
    16681 cattttgcaa tgtcattaag cactccgaat gacgatgcag tagccccaaa acctaatccc
    16741 atattattaa tacatgcagc cactgttgca aatgatgtaa gaaaatcata tcccatacca
    16801 tttaacacca gtataaaaaa caccgtgaag agagtataaa gaaaaaagaa actccataca
    16861 gacctcatta cacgatctgt aactatcttc cctcctacat ttacactcaa caacgctctg
    16921 ggatgagaaa gctgatttat ctcgtgtttg ctttgtttga aaagtataag aaatcgaagt
    16981 gacttaattc caccacaagt tgaacctata catcccccaa agaaacttga caacagcaaa
    17041 aacactatcg tgtgcgtggg ccaacttgca taatcctgcg tagctaaacc attatcagtg
    17101 agcatggagc tggcaagaaa aaacgaatga ataaaacttc catgcaagtc atacatacct
    17161 atatgccaga cctggaaaga ggtaacaatg atcaccccta aggctattaa cagaaagaaa
    17221 cgaagttcaa tatctctgat taaaggtttt atcgttttcc tgctaataac aatataccaa
    17281 agagtgaagt tgaaagccga tagcagggaa aaagaaccag ccaccagctc aaccaaatag
    17341 ttattaaaat atccgatact ctcgctatga gttgagaaac caccaagcga aactgtggaa
    17401 atcccgtgac aaatagcatc aaacaaaggc attcctgcaa gtctataaca gacaatacaa
    17461 gcaataccta ataaagaata agttatccac agtgtccgtg acgtatcggc caggcgggga
    17521 gtgagtttgt catccttaaa tggccccggc atttctgact gataaagctt tgcaccacca
    17581 atacccaata atggcaatac agcaaccgcc agaacaataa ctcctaaacc acctataaaa
    17641 tttaactgtg accgatagta caaatatgcc cgaggtaatg aactaacatc atcaattaca
    17701 gttgctcctg ttgttgttat tccagaaacc ccttcaaaca gagcatcaat gaacgttaaa
    17761 ttaagttctg agtcaatcca taaagggaat gcactaataa cagaaaacaa aatccaaaac
    17821 attacaatta taataaaccc atcacgggta cgtaattgaa tgccagattt cttagttgta
    17881 taccacgctc cgccaccaat gcaaaaaaat ataacgaaag ttataaagaa aacgaacagg
    17941 cttttttctt tataaaacaa tgctacaacc attggtggca acatt
//
LOCUS       DAOTRK010000097        17854 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.097, whole genome shotgun sequence.
ACCESSION   DAOTRK010000097 DAOTRK010000000
VERSION     DAOTRK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.097"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(51..398)
                     /gene="rplS"
                     /locus_tag="QIE41_003135"
     CDS             complement(51..398)
                     /gene="rplS"
                     /locus_tag="QIE41_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDS3531993.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(440..1207)
                     /gene="trmD"
                     /locus_tag="QIE41_003136"
     CDS             complement(440..1207)
                     /gene="trmD"
                     /locus_tag="QIE41_003136"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDS3531994.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKKEHAQQQHKHDGMA"
     gene            complement(1238..1786)
                     /gene="rimM"
                     /locus_tag="QIE41_003137"
     CDS             complement(1238..1786)
                     /gene="rimM"
                     /locus_tag="QIE41_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDS3531995.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(1805..2053)
                     /gene="rpsP"
                     /locus_tag="QIE41_003138"
     CDS             complement(1805..2053)
                     /gene="rpsP"
                     /locus_tag="QIE41_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDS3531996.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(2302..3663)
                     /gene="ffh"
                     /locus_tag="QIE41_003139"
     CDS             complement(2302..3663)
                     /gene="ffh"
                     /locus_tag="QIE41_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDS3531997.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRYITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            3830..4621
                     /locus_tag="QIE41_003140"
     CDS             3830..4621
                     /locus_tag="QIE41_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDS3531998.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            4642..5928
                     /locus_tag="QIE41_003141"
     CDS             4642..5928
                     /locus_tag="QIE41_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDS3531999.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5982..6575)
                     /gene="grpE"
                     /locus_tag="QIE41_003142"
     CDS             complement(5982..6575)
                     /gene="grpE"
                     /locus_tag="QIE41_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDS3532000.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPDASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            6698..7576
                     /gene="nadK"
                     /locus_tag="QIE41_003143"
     CDS             6698..7576
                     /gene="nadK"
                     /locus_tag="QIE41_003143"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDS3532001.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            7662..9323
                     /gene="recN"
                     /locus_tag="QIE41_003144"
     CDS             7662..9323
                     /gene="recN"
                     /locus_tag="QIE41_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDS3532002.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            9472..9813
                     /gene="bamE"
                     /locus_tag="QIE41_003145"
     CDS             9472..9813
                     /gene="bamE"
                     /locus_tag="QIE41_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDS3532003.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(9875..10165)
                     /locus_tag="QIE41_003146"
     CDS             complement(9875..10165)
                     /locus_tag="QIE41_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDS3532004.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDTVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10155..10592)
                     /gene="ratA"
                     /locus_tag="QIE41_003147"
     CDS             complement(10155..10592)
                     /gene="ratA"
                     /locus_tag="QIE41_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDS3532005.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTNNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            10763..11245
                     /gene="smpB"
                     /locus_tag="QIE41_003148"
     CDS             10763..11245
                     /gene="smpB"
                     /locus_tag="QIE41_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDS3532006.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            11460..11822
                     /gene="ssrA"
                     /locus_tag="QIE41_003149"
     tmRNA           11460..11822
                     /gene="ssrA"
                     /locus_tag="QIE41_003149"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11983..16557)
                     /locus_tag="QIE41_003150"
     CDS             complement(11983..16557)
                     /locus_tag="QIE41_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDS3532007.1"
                     /translation="MNRTSPYYFRRSVLSLLISALIYAQPGMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGRQEVYGGISNSSIIETGGEQLVSIHADINGQANNTTI
                     NGGRQSIEYGGISTGTIIESGNQYVYKGGTSNDTTIKGGTSRIEGGTANGTIIDGGGQ
                     SVSTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTAIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSSSDVIEVYSGGVLDVRGGT
                     ATNITQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVAENVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSEKANIKGGKQTVYGLATEANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMVNGTAEGSIINGGSQVVNEGGLAENSVLNEGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQDAANNILLADGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAVKTTINEGGTL
                     TVNDNGKATDIIQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQALARAEDLLIAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGVVRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DTSFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDP
                     KPDPTPAPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     TLSGHLGVGYSHGAGVESPWNAVAGANWSF"
     gene            complement(16622..16741)
                     /locus_tag="QIE41_003151"
     CDS             complement(16622..16741)
                     /locus_tag="QIE41_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDS3532008.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(16895..17038)
                     /locus_tag="QIE41_003152"
     CDS             complement(16895..17038)
                     /locus_tag="QIE41_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532009.1"
                     /translation="MWHLVVLLEELCKRGINFRALAQSIFPQQWGDECCKSKTICDLK
                     VIV"
     gene            complement(17492..>17854)
                     /locus_tag="QIE41_003153"
     CDS             complement(17492..>17854)
                     /locus_tag="QIE41_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532010.1"
                     /translation="PKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDLDT
                     PCIFNAPAYNKSNEKSLERVSENGLSGVLSDRNKKIKLITGVAPFDDILFMDDDFDDN
                     SPDDGPVENSPVVNSPLV"
BASE COUNT         4343 a         4748 c         4133 g         4630 t
ORIGIN      
        1 gccagccaat tggccagccc ttcttaacag gatgtcgctt aagcgaaacc ttagttaaga
       61 cgctctttga tacgagcagc cttaccagta cgctcacgca ggtagtacag tttagcttta
      121 cgaacagcac cacgacgttt gacagaaatg ctgtcaacta ccggagagtg agtctggaag
      181 acacgctcaa cgccttcgcc gttggaaatt ttacgaacag tgaatgcaga gtgcagaccg
      241 cggttacgaa tagcgataac cacgccctcg aatgcctgca gacgtttttt ggaaccttca
      301 acaacccata ctttcacttc cacggtatca cccggacgga aggaaggtac gtcctgcttc
      361 atctgctctt gttcaagttg cttaataatg ttgctcataa tttaatctct tatcctgggt
      421 aaactgatat ctcgggggct tacgccatcc catcatgttt atgttgctgt tgtgcgtgtt
      481 cctttttgaa ctccgccagc aaccttgctt gctcttcagt cagagccagg ttttccagaa
      541 gttcaggtct tctaagccag gtacggccca gcgactgttt caaacgccag cgacgtatct
      601 cggcatggtt gcccgacagt aacactggcg gaacttccat cccttctaac acctcaggcc
      661 gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga atcttccgtt gctgaggctt
      721 catgtcccag tactcccgga ataaaccggg aaacggagtc aatcagcgtc attgctggta
      781 actcaccacc actgagaacg taatcgccga ttgaccattc ttcgtcaatt tcggtttgga
      841 tcacgcgctc atctatacct tcgtagcgac cgcacaccag aatcaatttt tgattcgttg
      901 ccagttcgct gacgcccgct tgatcaagct tgcgcccctg tggtgaaaga taaatcacct
      961 ttgcgccttc acccgccgcg gcttttgctg catgaatggc gtcccgcaaa ggttgcacca
     1021 tcattaacat ccccggtccg ccgccgtaag gacgatcgtc cacggtacgg tgccggtcat
     1081 gcgtgaagtc gcgaggactc cagctctgga tgctcagcag gccattttta actgcccggc
     1141 cagttacccc gtaatcggta attgcgcgga acatttcagg aaacaggcta attatgccaa
     1201 tccacatagc gccgtctttt accgtttatc cggtggttta aaaaccagga tcccaatcta
     1261 cttcgattga acgagtagtg agatcgactt tcttgataac ctgcccatcg aggaacggta
     1321 cgagacgttc cttgatacca aacgcatctt tcaggtttgc cttaatgacg agaacgtcat
     1381 tagatccggt ttccatcata tcgacgactt taccgagatc gtagccttca gtggttacta
     1441 cctggcagcc catcaggtct ttccagtagt agtccccctc ttcaagctga ggcagctgcg
     1501 atgaatccac gacaatttca caattcgtca gcaggttcgc cgcatcacga tcgtcaacgc
     1561 ctttcagctt gatgatcatg tcctgattgt ggtgcttcca gctttccagc tggacttgct
     1621 gccactgacc cgccttctgg ataaaccagg gctgatagtc aaaaatgctt tcggcgtctt
     1681 cggtggaaga aaacactctg agccacccac gaataccgta agacgaaccc atttttccca
     1741 aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt gctcatcatg accaccgtga
     1801 cagattaagc tgctttgttt acttctttga tcagcgcagc aacgcgatca gaaatagttg
     1861 cgccctggcc aacccagtga gcgatgcgat ccagatccag gcgagtgcct tcttcttttt
     1921 cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa gcgaccgttg cgtgcattac
     1981 ggctgtcagc gacaacaacc tggtagaacg gacgcttttt agcgccgtga cgtgctaaac
     2041 gaatagttac cataacatcc tcttgtgtga ataaaacaac cggaccccat cgaggaacgg
     2101 agtccggtgt catattaaaa gcccgaaaat tttactcatt tttgcggaaa ttgcaatcaa
     2161 cagttgctaa ctctgctgta aaaggccgtc ggcggtgcag ccagtttggt gccggagtgc
     2221 gcgcagtcac cggagcgtac acgcagtacg tgaggatgac gagcacaccc cggtgccaaa
     2281 atgacaaaca agccaggccg attagcgacc aggaaatccc ggcggcatca tccccttcat
     2341 gcttctcatc atcttcgcca ttccgccctt cttcattttc ttcatcatgc gctgcatgtc
     2401 gtcgaactgc ttcagaaggc ggttaacgtc ctgtacctgc atcccgcaac cggcggcgat
     2461 acgacgctta cgcgaacctt tgatgatttc tggcttagcg cgctctttca tcgtcatcga
     2521 gttgatgatg gcttccatac gcaccagcac tttatcgtcc atctgcgact tgacgttatc
     2581 cgggatctgc cccatgcccg gcagcttgcc catcaggcta gccatgccgc ccatattttt
     2641 catctggcgc agctgctcaa gaaagtcgtt gagatcgaag ccgtcgcctt ttttcagctt
     2701 gctggctaat ttctctgcct gcgcgcggtc aactttgctt tcaatatctt cgatcagtga
     2761 cagtacgtcg cccatgccga gaatacgcga cgcgatgcgg tccggatgga acggctccag
     2821 cgcctcagtt ttctcgccaa caccgaggaa cttgatcggt ttgccagtga tgtaacgaat
     2881 agagagcgcc gcaccaccgc gggcatcgcc gtccactttg gtcaacacta cgccggtaag
     2941 cggtaacgct tcattaaatg cttttgccgt attggccgca tcctgaccgg tcatggcgtc
     3001 aaccacaaac agggtttcaa ccggattaat cgacgcatgg acttgtttga tctcgtccat
     3061 catcgcttcg tcaacgtgca gacgaccagc ggtatccacc agcagcacgt cgtagaattt
     3121 cagtttggct tctttcagcg ccgcgttaac aatatcaacc ggcttctgac caacatcaga
     3181 agggaagaaa tcaacgccca cctgctctgc cagcgtctca agctgtttga ttgccgccgg
     3241 gcgataaacg tcggcggaaa ccaccagcac tttcttcttg tgcttctcgc gcaggaactt
     3301 accgagttta ccaacgctgg ttgttttacc ggcaccttgc aggcctgcca tcagcacgac
     3361 cgcaggcggt tgcgcagcca ggttcagggt ctggttctct tcgcccatcg ccgcaaccag
     3421 ttcgttacgg acgattttga cgaactcctg ccccggcgtc aggctcttat taacttcatg
     3481 accaaccgct ttctctttta cgcgattgat aaactcacgc actaccggca gagcgacgtc
     3541 cgcctccagc agcgccatgc gcacttcgcg cagcgtatct tttacgttgt cttcagtgag
     3601 gcgtccacgg ccactgatat tgcgcagcgt gcgcgacaaa cgatcggtta aattatcaaa
     3661 cattgtctct cgcctggggt ggaaacggtt ggccgcaatc gcgacacatc atcagtattt
     3721 tgccgcagta taacatgaag gcgtctttgt tgttatgcaa cggttggagc agcgttcacc
     3781 tgacgctata ctgcttctct ttcttattgc tcaaactgtc gacattacta tgcccgtttt
     3841 tgctctgctc gcgcttgtcg cctactccgt cagtcttgcg ctgattgttc ccggtctgct
     3901 gcaaaaaaac ggcggctggc ggcgcatggc tattatttct gcggtcattg cgctggtctg
     3961 ccacgcaatc gctctggaag cccgcatcct gcccgacggt gatagcggac aaaacctcag
     4021 cctgctgaac gttggttcat tggtcagttt gatgatctgt acggtaatga ccattgtggc
     4081 ttctcgcaat cgtggctggt tgctgctacc cattgtctat gcctttgcgc ttatcaacct
     4141 ggcgctggcg accttcatgc ccaatgaata catcacccat ctggaagcaa cgcctgggat
     4201 gctggtgcac attggcttat cgctcttttc ctatgccacg ctaattatcg ccgccctgta
     4261 cgcgctgcaa ctggcgtgga ttgattacca actgaagaac aagaaactgg cgtttaacca
     4321 ggaaatgccg ccattgatga gtatcgagcg taaaatgttc cacatcacgc agattggcgt
     4381 ggtgctgcta acgctcacgc tttgcactgg cctgttctac atgcacaact tatttagcat
     4441 ggaaaatatc gacaaggctg tgctctctat cgtggcgtgg tttgtctata ttgtgctgct
     4501 gtggggacat tatcatgaag gatggcgtgg acgccgcgtc gtctggttta acgttgcggg
     4561 tgcagtcatt ctgacactgg cctacttcgg cagtcgaatt gtccagcagt taatcagcta
     4621 aacccagaaa aggagtttcc cctggaacac atttctacta ctacgttgat cattattctg
     4681 atcatcatgg tggtcatttc agcctatttt tccgggtccg aaaccggaat gatgaccctc
     4741 aaccgctatc gtctgcgaca tatggcgaaa cagggtaatc gctcggccaa acgcgtcgaa
     4801 aaattgctgc gtaagccaga ccgcctgatt agcctggtgt taatcggcaa taacctggtc
     4861 aatattcttg cctccgcgct cggcactatt gttgggatgc gtttgtacgg cgatgcgggc
     4921 gtggcaattg cgactggtgt gctgactttt gtcgtactgg tttttgctga ggtattgccg
     4981 aaaaccattg ccgcgctgta cccggaaaaa gtcgcttatc cgagtagttt tctgctggct
     5041 ccgctgcaaa ttttgatgat gccgctggtc tggttgctga atgctatcac ccgtatgctg
     5101 atgcgcatga tgggtatcaa aaccgatatc gtggttagcg gctctttgag caaagaagag
     5161 ttgcgcacta tcgtgcacga atcgcgctca caaatttccc gtcgcaatca ggatatgctg
     5221 ctgtcggtgc tcgatctgga aaagatgacc gttgatgaca tcatggtgcc gcgcagtgaa
     5281 attatcggta ttgatatcaa cgatgactgg aaatcgattc tgcgccaact atcccactca
     5341 cctcacgggc gcatcgtgct ctaccgtgat tcgctggacg acgccatcag tatgctgcga
     5401 gtacgtgaag cctggcggtt gatgtcggag aaaaaagagt tcaccaaaga aaccatgctg
     5461 cgcgccgcgg acgagatcta ttttgtcccg gaaggtacgc ccctcagcac gcagttggtg
     5521 aagtttcagc gcaacaaaaa gaaagtcggc ctggtcgtca acgagtatgg agacattcaa
     5581 gggctggtga cggttgaaga tattctggaa gagatagttg gtgatttcac cacgtcgatg
     5641 tcgccaacac ttgccgaaga agtcactccg caaaacgacg gttcggtgat tatcgatggc
     5701 accgccaacg tgcgggaaat caacaaagcc tttaactggc atctaccgga agatgatgcc
     5761 cgcacggtta atggcgtcat tcttgaggca ctggaagaga tccctgtcgc aggcacccgc
     5821 gtgcgtattg gcgagtacga tatcgatatt ctcgacgtac aggacaatat gattaagcag
     5881 gtaaaagttt ttcctgtaaa accgctgcgc gagagtgtgg cggagtaaaa aaacggcccg
     5941 gcattcgcat gctgggccgt tatttattac gaaaacagaa attaagcttt tgccttcgct
     6001 acagtcacca tcgccgcacg aattgtacga ccgttcagcg tataaccctt ctgcataatg
     6061 cccagtacgt tacctggcgc aacgtcatca gattccacca ttgcgatggc ctgatgcaca
     6121 ttcgggtcca gcgggacgtt agtttcggcg atcacttcaa cgccaaactt acgcacaaca
     6181 tccagcatcg acttcagcgt cagctcaata ccttcaacca tcgcggacat atccgggtta
     6241 gctttatcag ccacttccag cgcacgatcc aggctatcaa tcaccggcag caattcgttg
     6301 atgaatttct ccagcgcgaa tttgtgggct ttttcaatat ccagttcagt acgacgacgc
     6361 aggttttcca tttcggcttt tacgcgcaaa atgccgtcac gttcacgagt ctgggcttca
     6421 gccagctgag cttcgagatt cgcaattttt tcatcgcgcg gatccacctg ctcagcagaa
     6481 gcgtctggct caactgcctc aatctcttcg tgctgatcca tgataatttc ttccggggct
     6541 tgcccctcag gcgttttctg ttctttacta ctcatgaatt tctccgcgtt tttttcgcat
     6601 tcatctcgct aacttcgctt attatgggga tcagtttcag ggtttcaagg gaagcactca
     6661 cattgtcatc aatcttcgca acaaggacct cggaaaaatg aataatcatt tcaagtgtat
     6721 tggcattgtg ggacacccac ggcaccccac tgcactgaca acacatgaaa tgctctaccg
     6781 ctggctgtgc acaaaaggtt acgaggtcat cgttgagcag caaatcgctc acgaactgca
     6841 actgaagaat gtgaaaactg gcacgctcgc ggagattggg caactggctg atctcgcggt
     6901 agtcgttggt ggcgacggta atatgctggg cgcggcgcgc acactcgccc gttacgatat
     6961 taaagttatt ggaatcaacc gtggcaacct gggtttcctg actgaccttg accccgataa
     7021 cgcccagcaa caattagccg atgtgctgga aggccactac atcagcgaga aacgtttttt
     7081 gctggaagcg caagtttgtc agcaagattg ccagaaacgc atcagcacgg cgattaacga
     7141 agtggtactt catcctggca aagtggcgca tatgattgag ttcgaagtgt atatcgacga
     7201 gatctttgcg ttttctcagc gttctgatgg cctgattatt tcgacgccaa caggctccac
     7261 cgcctattcc ctctctgcgg gcggtcctat tctgacaccc tctctggatg caattaccct
     7321 ggtgcccatg ttcccgcata cgttgtcagc gcgaccgctg gtgataaaca gcagcagcac
     7381 gatccgtctg cgtttttcgc atcgccgtaa cgacctggaa atcagttgcg acagccagat
     7441 agcactgccg attcaggaag gtgaagatgt cctgattcgt cgctgtgatt accatctgaa
     7501 tctgattcat ccgaaagatt acagttattt caatacatta agcaccaagc tcggctggtc
     7561 aaaaaaatta ttctaatttt acgccagcct ctttactgta tataaaacca gtttatactg
     7621 tacacaataa cagtaatggt ttttcataca ggaaaacaac tatgttggca caactgacca
     7681 tcagcaactt tgctatcgtt cgtgagcttg agattgattt tcatagcggc atgaccgtaa
     7741 taactggcga gaccggcgcg ggtaaatcta ttgcaataga tgccctcggc ctttgtctcg
     7801 gtggtcgcgc tgaagccgac atggtgcgta ccggcgctgc tcgtgccgac ctgtgcgccc
     7861 gtttttctct gaaagatacg ccagcggccc tgcgctggct ggaagaaaac cagcttgaag
     7921 acgggcatga atgtttgctt cgtcgcgtaa tcagcagcga tggtcgctcc cgtggtttta
     7981 tcaacggtac agcggttcct ctttcacaac tgcgcgaact gggtcagttg ctgattcaga
     8041 tccatggtca gcacgctcat cagttactca ccaaacccga gcaccaaaaa ttcctgcttg
     8101 atggctatgc caatgaaacc tctctactcc aggaaatgac cgcacgttat cagttgtggc
     8161 atcaaagctg ccgtgacctc gcgcatcatc aacagttaag tcaggaacgc gccgcccgtg
     8221 cagaactgct gcaataccaa ttaaaagaac ttaacgaatt taatccgcag cccggagagt
     8281 ttgagcaaat cgacgaagag tacaaacgtc tggcgaacag cggtcaattg ctgactacca
     8341 gccagaatgc attggcagta atggccgacg gtgaagacgc aaacctgcaa agtcagcttt
     8401 acacggctaa acaactggtg agcgaattga ttggcatgga cagcaaactg tccggcgtac
     8461 ttgatatgct ggaagaagct accatccaga ttgctgaagc cagcgatgaa ctgcgccact
     8521 actgcgatcg tctggacctc gatcctaacc gactgtttga acttgaacag cgcatctcaa
     8581 aacagatttc gctggcacgt aaacatcacg tcagccctga ggcattgcca cagtattacc
     8641 agtcgctact ggaagaacag cagcaactgg acgatcaggc cgactcacaa gaaacgctcg
     8701 cgctggcggt aacgaaacat catcagcagg cactggaaac ggcgcgcgcg ttacaccagc
     8761 aacgccagca ctatgcagaa gaacttgcac agctgatcac cgacagtatg catgcactct
     8821 ctatgccaca tgggcagttt acgattgatg ttaaatttga cgagcatcac ctgggcgctg
     8881 acggtgccga tcgtattgag tttcgggtaa ccaccaaccc aggtcagcca atgcagccta
     8941 ttgccaaagt tgcatccggt ggtgaattgt cccgcatcgc attggcaatt caggtcatca
     9001 cggcgcgtaa aatggaaacc ccggcactga tttttgatga agtggatgta gggattagcg
     9061 gcccaacagc ggcagttgtt ggcaaactgc tgcgtcaact cggcgaatca actcaggtga
     9121 tgtgtgttac ccacctgcca caagtcgcgg gatgtggtca tcaacactat tttgtcagca
     9181 aagaaaccga tggtgcgatg acagaaacgc atatgcaatc cctggataaa aaagcgcggt
     9241 tacaagagct ggcgcgcctg cttggtggca gtgaagtcac acgtaacaca ctggcgaatg
     9301 cgaaagaact gcttgcggcg taaacttttt tcctgcttca cggtcagagt aaacagcaaa
     9361 acgccgtaag accggaaagc aaaaggtttt aaagtgatga aaggtctatt atcatcggca
     9421 tattacagat gagccacgta ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt
     9481 aaaacgctga ctgctgcagc agcagtacta ttgatgttga ccgcaggctg ttccactctg
     9541 gagcgagtgg tttaccgtcc tgacatcaac caggggaact atctgaccgc taacgacgta
     9601 tccaaaatac gtgttggcat gacgcaacaa caagttgcgt acgcattggg tacaccgctg
     9661 atgtccgatc catttggtac gaatacctgg ttctatgtct tccgccagca accaggtcat
     9721 gaaggtgtaa cgcagcaaac gctgacgctg acctttaaca gtagcggagt gttgaccaac
     9781 attgataaca aacccgcgct gagtggtaac taatcataaa gttgccctca aagacgttaa
     9841 aaaaggtgct caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc
     9901 gttgcctgcg cagctctttc ggatcggcaa tgagaggacg ataaatctcc acccgatcgc
     9961 catcatgcac ggtatcgctg agttttgccg gacggctgta aatgccgact ttgtttttag
    10021 ttaaatcgat atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg
    10081 tcgcgccctc ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca
    10141 cctcaacggc aattttacct ggcactgtaa acctctttcg cccgaaccgt aaaagcctgg
    10201 accatattag ccgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg
    10261 gtaaactcaa agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac
    10321 ttccagccac cgatcaattt cttgaacggc ccatccacca gattcataag aatactttgg
    10381 ttattggtca actggttgcg ggtagtaaac gttttgctga tcccggcctt agagacatct
    10441 accgccgcag tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc
    10501 aaaaactgag gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag
    10561 ggtaccagtg cggtccggct aatctgaggc ataacaattt ccatcaacaa aaatccaaca
    10621 aataatatca tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt
    10681 tcatgctaag atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga
    10741 ttaccgatga ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac
    10801 catcgcgctt aacaagcgcg cccggcacga atactttatc gaagaagagt tcgaagcggg
    10861 acttgccctg caaggctggg aggttaaatc cctgcgcgca ggaaaagcca atatcagcga
    10921 cagctacgtc cttctacgtg atggagaagc atttctgttt ggcgctaaca tcacgccaat
    10981 ggctgtggcc tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa
    11041 ccagcgcgaa ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc
    11101 gctctccctg tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa
    11161 gaaacagcac gataaacgtt cagatatcaa agagcgcgaa tggcaggtgg ataaagcgcg
    11221 tatcatgaaa aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc
    11281 gcctccctct ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt
    11341 tgccatccca ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga
    11401 taacgccatt gaggctggtc atggcgctca taaatctggt atacttgccg ttacacattg
    11461 gggctgattc tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg
    11521 gcctcgtaaa aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta
    11581 ataacctgct tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg
    11641 tcaaacccaa aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc
    11701 aggctagttt gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta
    11761 agcatgtagt accgaggacg taggaatttc ggacgcgggt tcaactcccg ccagctccac
    11821 caatcatgat tggacggtgc aaggacaaca ccaacaaaaa caggaagtta gaagtctcag
    11881 caagacaccg accagacggt gaggagacat aaaaggatac gcaaaggagc cgcggctctt
    11941 ggtgatatga aagcccacag atgtgggctt tttcgttgat ggtcagaacg accagttcgc
    12001 accagccacc gcgttccacg gggattccac accggcacca tggctatacc ccaccccgag
    12061 atgtccgctt aacgtactgc tgaatgaggc tttaatacct gcctggtata ttccacgtct
    12121 gcccgacaaa tcattgacga aattaccgtc attattcacc ttcacccggt tatcatcgac
    12181 aaattctttg cgcacagccg ccttcagcca cggctcaatt tccataccgt tccccagacg
    12241 catgttgtaa ctcagcgttg cgcccagttc acgatatata ctgcgggtat cgactgattt
    12301 cgattccatg ccattggata aatgatattc ggggttatca gcggtgaacc ccgttaacga
    12361 tgcatacggc gtcaggttcc agttaccatc cgtaaatcgc atcccggttt caatgtgacc
    12421 gcccagcccg ttgctgcgat aactgccgtt ggcggctcca ccgctgctca ttttaccggc
    12481 tacgttactt tcaaagcggt tcagcttcac gataccgtcc agatagaaac cactttcatg
    12541 ttcccagctg gcatagcccc ccagagaata actgcccaca ctgccatgtc ctccacgatc
    12601 aaaaccaata tgtgaatggg aataacccat aaaagcgccc agcgtcgcaa tcccctcagg
    12661 aatatcatta cggctatcga tccccaccgt cattccggtc agcgtctgct caaacccggc
    12721 acccgcatcg gtggtgacat tattacgggt gttatacgtc gtcccccaga cattattgtt
    12781 gtgtggactc gctttcatta tgttcaaccg ctcgcgaata ctgtttagct cagcatcaaa
    12841 gaccaacggt aatgttgctg ccatattgag tacggctgct gtagaaggcg tgatgcgttt
    12901 ctccgggacg ggtgtcggcg ttggagcggg agtcgggtct ggtttcggat ccggttttgg
    12961 gtctggtttt ggatccggtt tcggatttgg gttggggtcc gggttgggtt tgacatcatt
    13021 ggtcaggttc cagttgctgt tgccgtcgct tttcaggaca tactcatagg tcccaagatc
    13081 aacgaaaccg ccggtattac ccagcgtaaa cgaagtatcc cctccccctg ttttcaccag
    13141 cgtcatcgcg tcgtcagact gagggctgac gccggtatcc tgaacaaaga ttttaaaatt
    13201 gccagtggcg ttgttgttga cgaccagttg atcgccccgg gagcctgcaa cgttggtatg
    13261 caggtagaaa ttaccgctgc cggaaagttc actggtggtc agcgtattgt agataccgtt
    13321 agttgtggca ggcgctgctg tttgtgctga taaataaaca tcaccgtcgt tgaggagtaa
    13381 actgtttact ctatattcgc agttgctggt gcctgcacag gaattattgc tgttaagcca
    13441 gacactgccc cgactggcag ccgtcaggtc ggcaagcgtc gtatcgacgc catttccgat
    13501 agttaatgtc gcgctatcgg taatccggac caccccttca agtttaactg gcgtaacatt
    13561 atcccgtggc gtcattaacg acaggcttcc tgtcgcgcca ctgaccgatg catccgccag
    13621 cgtacctgcg tagacgattg ccgtcccgcc agcgatcagt agatcttctg cccgggccag
    13681 agcctgaaac tcatcttttg accgaccaag ggtatattgc ccaccggaat taacctttgt
    13741 ggcgaagtcc tgacccaggt tttgcattgc cccaccgttg ccaaccgtgg aatcgcgagc
    13801 ttcggtacct gctaatacca ataaattacc gccattttcc agcagcacat tggtcgctaa
    13861 atttccggca atggaaaagg tgccgtactg gtgagtaccg ctgatttcaa taccgttagc
    13921 cgtgctcgtc tggagagcgg caccgctgtt ctggatgata tctgtcgctt tgccattatc
    13981 gttgactgtc agcgtaccgc cttcattaat cgttgttttt actgcctcac cgttagccga
    14041 aactgtttgt attccgccgt cgttaatcgt tgtctcattc gccaccccct cgacgatttg
    14101 ttcgccgccg gtgagcgtcg tgcctgtcgc agtggctttt gttttgacga tctcccgccc
    14161 gcccgcattg acctgtgttt tggcggaagt ggtgtctgac tccacggtta acacgccgcc
    14221 atctgccagc aggatattgt tcgccgcatc ctgctcaatg ctgaacgcga cgccatcagc
    14281 gcgtgtccct gtgacccgcg tcgctctggt ggttgcaacc aacgcgccct ggctactttg
    14341 ctgtatcccc gttgcgctgc ctttctcccg cacatcgaga gtgccgcctt cattaagcac
    14401 cgagttttca gccagaccgc cctcattaac tacctgtgaa ccgccattaa taatggaacc
    14461 ttccgctgtc ccgtttacca taatttgttg taggccagag acgatatcgg tattgatcgc
    14521 cttaccataa ttctgaacgg tttgcgtacc gccattgatg tgcgttttgt ctgttgaccc
    14581 accatcaaca atttgttcac cactttcgat atttgcctca gtggctaaac catataccgt
    14641 ttgcttgcca cctttgatat ttgctttttc agaagtggca ctggcatata ttgtttgggt
    14701 accagcacta ttaagtacag taccaacatc ttttccataa acatccattt tgccgttggc
    14761 attaataatc gtgtcaactg cccgggaacc agtgacgact gttaatgagc cagcgttttc
    14821 cagcactaca tttttagctt ctgaattcct gatgtagaaa gcatcaccat aactgttggt
    14881 tccttcgata agtgttccgg aggtcgtgga agcaattaat gcgccgccgg attgttgctc
    14941 aacatgctta gcctcaccac cgtcctgaac ctccagaacg ccaccattat taagtctggt
    15001 gctatctgtt ttagcctcct tctggacaat cagcttaccg ccagcatcaa tggtagtatt
    15061 tttcgccgag gttttagcca ttaccgtcag ttcgccggta ttttccagca caacataatt
    15121 agcctctcct ccggtaatag tgaagtgaga gagcttgttg tatccttcga tatcagtccc
    15181 tgcgcccgtg ttggcaacta aagcactgcc cgtctcctgg ttaaccccat gtgcaatacc
    15241 gccggtatag acaatcagcg agcctccggc gctaatattg ctgccagttg ccgtaccatc
    15301 tttctcaaca acctgccggc tcccagagga tatatttgtc gtgtcagctt tcccgccgct
    15361 cttgatattt tgcgttccac tgttgatatt ggtgcccgtg gcaataccat gattattaat
    15421 attctgtgtg ccaccattaa ttatggtatt tgtcgcgttt ccggcaacat ccataacgcc
    15481 gccattatct attcgggtcg catcagcttt agcattcgtt aaaactgaca ttgttccttt
    15541 atctttaata atcgtcttat ttgccgaacc atatgcgttt atgtctaaat gaccaccgtt
    15601 ttccagcaac acattctctg ccacgttatt gtggatggag aatgcccctt cactattcgt
    15661 accgctcacc gtcaaatcgt aagtcgttac ttttaaagcg gctccatcgt gctgagtaat
    15721 attagttgcc gtaccacctc taacatcaag cacgccgccg gaataaactt caataacatc
    15781 cgaggagctc gtgttatcaa caatttgcgt accaccagaa tagatctgcg tattttttgc
    15841 cgttgactta ctattcagag actgagttcc gccttcaatc gtcgtgtcca gcgcacggct
    15901 ctcatatact ctttgctcac cgccattttt aatggcggtt gtttctactg tgctctgttc
    15961 aacatactgt cgcccaccat ttatggttgt gttcgtcgcc agacttcctt gcgtgatgtc
    16021 ctgataacca gacttattta tcgtcgtacc gtcaacatgc ccctgagttg atactgactg
    16081 gcctccacca tcaatgattg ttccatttgc agtccccccc tctatgcgtg aagtaccgcc
    16141 cttaattgtc gtgtcattgc tggtaccacc tttataaaca tattgattgc cactttcgat
    16201 tatcgtgcca gtagaaattc ctccgtactc aattgactgt ctgcctccat taatggttgt
    16261 attattagct tgcccgttaa tatcggcatg gattgatact agttgctcgc caccagtttc
    16321 aataatacta ctattagata tcccaccata gacctcctgc cggccatggt tgataatatg
    16381 agtgttattt gttgtacctc gttcatctac tcgttggttg ccatctacag tctcatcgtt
    16441 taccacacca ataacattag tagtgaaggc cgccatcccg ggctgggcat atattaaggc
    16501 agatatcaat aaggaaagta ctgagcggcg aaaataatag ggactggtcc tgttcataaa
    16561 tttcatcctc tgaaaagtga atactgagta gcgtttaagc gaccttagct ttgctgcaac
    16621 atcagcccac aggcaccaga ccaggggatt catcctgaag agacagcgca agtgtattgt
    16681 gttcaccgct catcaaagac atcatgatga aatgatgata ttccgcataa gaatgaggca
    16741 tttttttaag acattgtgct gaaggatgat tggataaaaa agtcaatctt ttcaggaaat
    16801 acggcgtatt cttttctttc gacattgaag ccgtcggaaa aataaaatga tttacataat
    16861 cgtttctgat gaatatcttc tgctcacata aaaatcacac aataactttg agatcgcaga
    16921 ttgttttact tttacagcat tcgtcccccc attgttgggg aaatatagat tgggccagag
    16981 cacgaaagtt aataccacgt ttgcacagct cctccaacag cacgacaaga tgccacatac
    17041 tgcgccccag tcggttcagt ttacagacta gcagtgcgta ccctgccgat aatgtcctga
    17101 gcagtttttc agtccgggtc tgctgccacg gttatgcgct gaagtggtaa ctctgagcac
    17161 gcagttcatc agtcaggcgt ggtacaccgt aacgctgtta ttgctgggta atatcaaaaa
    17221 aactttcagg cagctaagga aagttgaacc agacattaga tgaaatattt caaccaatta
    17281 cagcaccaat tcagtcactg ctagcccacc aaataaatca aggggttaca tgaaaacgta
    17341 gccccttttt ctttggtagt gacactaaaa tggatgtagt gtgaagaata accccattta
    17401 cttaatcaat aatacatatt gtttcaatct acgttattat ctcttcgtaa aaatagccat
    17461 ttatttatca ttgaaaacta cttttagaac ttcatacaag gggactattc acaacaggac
    17521 tattctcaac gggaccatca tcaggggagt tatcatcaaa gtcatcatcc ataaataaaa
    17581 tatcatcgaa tggtgccaca cccgtgatta gttttatttt tttattacga tcagacaaga
    17641 ctccacttaa accgttttcg ctgacgcgtt ctaatgattt ttcattactc ttgttgtaag
    17701 caggcgcatt aaaaatacat ggagtatcaa gatcaaacaa tgacgttccc cagttcacat
    17761 attgaatatc atagttactg aagttctgtc cagaaaagaa gcatccctta aaatccaatc
    17821 cacgtaaatt atataaacca ccctcttctt ttgg
//
LOCUS       DAOTRK010000098        17849 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.098, whole genome shotgun sequence.
ACCESSION   DAOTRK010000098 DAOTRK010000000
VERSION     DAOTRK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.098"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..2415
                     /locus_tag="QIE41_003154"
     CDS             <1..2415
                     /locus_tag="QIE41_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDS3532011.1"
                     /translation="AAGILSECRTQLVAEECEYATADMLPELFVAPPWVINKKTNVIP
                     VFDLPVLPIPAVTDITPGITELISHTDISRFSEIAQYQSSQQTLFTDLPLIEKESWET
                     SFIPFTPEQQILWQLGFNEWLHCEDDLHEKKYIPQSAVDALLRFDFPALKAEFAKYHN
                     NANKSWNLSALCYLPGQQAISFLNQIIIEERYSGEKEILAVFGSTAIPAFMTCLQRDH
                     QRLWIFTLFIGASELALPMAQRLQKKMAYKDAINWLANNPRHAAAGLLPLALGKPCQN
                     REYARQAFRLLVKLNQRETIEEIGRRYNQPDVLAALATLFDSDPLEEYPAKIAPLPGF
                     YQFTLWRRPRLKSNNLPLPDDAMRHLGTMLSFPRDITSYAGLATIKETFTHESLADFG
                     WDLYTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRAWPGESQHKRAVYGLDVL
                     ADIGSDVALMLLNGIARKIKFKALQEHAREKINIVAENRGLTMAELEDRLAPDLGLDN
                     SGSLVLDFGPRQFTVGFDETLKPVVHDANGKVLKDLPKPNQSDDQTLATDSVNLFKQL
                     KKDVHAIASQQIDRLEQDMCQRRRWTAEQFRLFLVEHPLVRHLTRRVLWGVYTEENTL
                     IACFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESATAFGQIYADYEKLPPFRQ
                     LDRGYYHLTDNERDTHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYG
                     ALELETEHFSLIYGETGYSDLLPVESVKITSPGERYSTQPSLTFSALDAITASELIND
                     IESLFD"
     gene            2428..6222
                     /locus_tag="QIE41_003155"
     CDS             2428..6222
                     /locus_tag="QIE41_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDS3532012.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELNISWGKVGTNGQSQVKSFAD
                     AAAAEKAELKLIAEKTKKGYVEHAENATSKHTRTVTKSLELSDFSTIIQEQPSVTPEF
                     YVADKNTDVILPWLAKDIAVVFPLEVVPTTLSHRRFPGDPVTPVDKLTQLRRLACGVS
                     QRDNKTATFDFSACTLEWQNFIAQAIDLINSPKTTKLPSPVMAVLAALELHCTRYKER
                     ADVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAVPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     VASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPISYLTEEISNQLLAKYIWYSKHITVSHEESTA
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLISEFKVFHSPTGHYWHLGILTTL
                     PLEKAVRAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARTWLLKYPEHAITGLLSAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNAMLALDPLDNHPTKIPTLPAFYQPSIWTRPVLKAN
                     AQSLPDSALQHLGEMLRFHQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAISSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPALGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYST
                     ADDDLFTLQEGDISVGIPHVLEISPTDAVAFGQLFADYELLPPFRQLERNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPADLSAEQVLSKLWLWEGKAESYGWGSNSTQEAQFSVLDDITASELINDIEAL
                     FE"
     gene            6232..9864
                     /locus_tag="QIE41_003156"
     CDS             6232..9864
                     /locus_tag="QIE41_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDS3532013.1"
                     /translation="MDKELPWLADNAQVELKYKKGKTPLSHRSWPGEPVPVITESLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPWTHFHSKEWLKVVAIDPRAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPTRPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMPWLSTQARSVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTVPRLDLAPF
                     ELTPQVYWQPGERERLAATESARYFSTESLAERMEHKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRREKSIWNLYLLAQLPREIALTFWLRINEKKHLFAGEDYFLSIFGLDALPGLL
                     LAFSYRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPT
                     RIPKAPDFWQPAMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTQMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEQLADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYENAF
                     VYDLGEYRLVLKFSSGFNHYNVDSKALMSFRSLHVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            9925..10245
                     /locus_tag="QIE41_003157"
     CDS             9925..10245
                     /locus_tag="QIE41_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532014.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDVDPLINDALIYIRDEFIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIEK"
     gene            10821..11898
                     /locus_tag="QIE41_003158"
                     /pseudo
     CDS             10821..11898
                     /locus_tag="QIE41_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            11909..14188
                     /locus_tag="QIE41_003159"
                     /pseudo
     CDS             11909..14188
                     /locus_tag="QIE41_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14181..15317
                     /locus_tag="QIE41_003160"
     CDS             14181..15317
                     /locus_tag="QIE41_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDS3532015.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGCSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            15314..17314
                     /locus_tag="QIE41_003161"
     CDS             15314..17314
                     /locus_tag="QIE41_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDS3532016.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQTTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPSEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     DACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNNRLNCAQEMARRDSVGEVPPMPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     VFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            17439..>17849
                     /locus_tag="QIE41_003162"
     CDS             17439..>17849
                     /locus_tag="QIE41_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDS3532017.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITM"
BASE COUNT         4543 a         4582 c         4506 g         4218 t
ORIGIN      
        1 gcggcaggca tcttatctga atgtcgtact cagttagtcg ctgaagaatg tgaatacgcc
       61 actgcggata tgttgccgga gctgtttgtc gctccaccgt gggtaataaa taagaaaacg
      121 aatgtaatac ccgtatttga tttaccggtg cttcccatcc ctgcagtcac tgacatcacg
      181 ccaggaatta cagagttaat cagtcatact gacatatcca gattcagtga aatcgcgcaa
      241 tatcagtcat cacagcaaac cttatttacc gacctgccac taattgaaaa agaaagctgg
      301 gagacatctt tcattccttt tacaccagaa caacaaatcc tttggcaatt aggttttaat
      361 gaatggctgc attgcgaaga tgacttacat gaaaaaaaat atataccgca aagcgctgtt
      421 gatgccttgt tgcgctttga ttttccagca ctaaaagctg agtttgcgaa atatcacaac
      481 aatgccaaca aaagctggaa tctatctgcg ctttgttact taccgggaca acaggcgatt
      541 tcatttctca accaaataat cattgaggag cgatattcag gtgagaaaga gattctggca
      601 gtttttggta gtacggccat tcctgcgttt atgacctgct tgcaacgaga tcatcaacga
      661 ctgtggatat tcaccctctt tattggggcc agtgaactag ccttaccaat ggcgcagcgg
      721 ttacagaaaa aaatggctta caaggatgcc attaattggc tggcaaataa cccgcgccat
      781 gccgccgccg ggctactgcc tttggcgctg ggcaaaccgt gccaaaatcg ggaatatgcc
      841 cgccaggcat tccgcctgct agtgaaatta aaccagcgag aaacgattga agaaatcggc
      901 cggagataca accaacctga tgttttagct gcgctggcaa cattgttcga tagcgatccg
      961 ctggaagaat atcccgctaa aatcgcccca ctgcccggct tttatcagtt caccttatgg
     1021 cgcagaccac ggcttaaaag taacaacctg cctctgccag atgacgctat gcgccacctg
     1081 ggcacaatgt tgagctttcc acgcgacatt acctcctacg ctgggctggc gaccatcaaa
     1141 gagaccttca cccacgaatc actggctgac tttggctggg atctctacac ggcatggaca
     1201 gaagctggcg cacccgcaaa agaaaactgg gcatttactt cgcttgggat tttaggcaat
     1261 gacgacaccg cacgtaaatt aaccccgctt atccgtgcct ggccgggtga atcccagcat
     1321 aaacgcgcgg tttacgggct ggatgtatta gctgatattg gcagcgatgt tgcgctaatg
     1381 ctgcttaatg gcatcgcgcg aaaaattaaa ttcaaagcat tacaggaaca tgcccgcgag
     1441 aaaatcaaca tagttgctga aaaccgtggg ctgactatgg ctgaactgga agaccgcctg
     1501 gctccagatt tagggcttga taacagtggc tcgctggtac tggatttcgg cccacgtcaa
     1561 ttcaccgttg gttttgacga aaccttaaaa cctgtagtgc acgatgcaaa cggcaaagtc
     1621 ctgaaagatt tacccaaacc aaatcagagc gatgatcaaa cactggcaac tgactccgtt
     1681 aacctcttca aacaattgaa gaaagatgtg cacgccattg ccagccagca gattgatcgt
     1741 ctggagcagg atatgtgcca gcgcagacgc tggacggcag agcagttccg cctgtttctg
     1801 gtggagcatc cgctggtacg tcacttaacc cgacgggtgt tatggggggt atatactgag
     1861 gaaaacacgc tgatcgcctg cttccgggtc gcagaagaca gcacttacag cgatgcgcag
     1921 gatgagttat tcacgctgcc agcaggaaac atcggtatac cgcatgtgct ggaaatatct
     1981 cctgaatccg ccactgcatt tgggcaaatt tatgctgatt acgaaaagct tcctcccttt
     2041 cgccaactcg atcgtggtta ttaccacctt actgataatg aacgtgatac ccatgaattg
     2101 atacgctggc aaggacgtct ttgccaggcc gggcgcattg tcgggctgga acgtcgaggc
     2161 tggcaacgtc tggaagaaag cggcagcgtc tatgcgatgc gcaaatctac cccttatggc
     2221 gctctcgaac ttgaaacaga acatttttca ttaatttatg gcgaaacggg gtatagcgac
     2281 ctgctacctg ttgaaagcgt aaaaatcact tcaccagggg aacgctacag tacacagccc
     2341 tcgctcacgt tctccgcgct tgatgccatc accgccagcg aactcattaa cgacatcgaa
     2401 tcactttttg attaaaagga ctttgcaatg aaaacctata tttatcagga cgaaaaatca
     2461 cataaattct gggcggtgga gcagcaggga aacgaactga atatcagttg gggcaaagtc
     2521 ggcaccaacg ggcaaagtca ggtaaaaagc tttgcagatg ctgcggcggc ggaaaaagcg
     2581 gaactcaagc tgattgcgga gaaaacgaaa aaggggtatg tggaacatgc ggagaatgct
     2641 acatcgaaac atactcgaac ggtaacgaaa tctctcgaat tatctgactt ttctaccatc
     2701 attcaggaac agccctcagt tacaccagaa ttctacgtgg ctgacaaaaa tacagacgtt
     2761 attttaccgt ggctggcaaa agatattgct gtcgtttttc cgcttgaagt cgtacccacc
     2821 accttaagcc atcgccgctt tcccggagat cctgttacac ctgtagataa attaacccag
     2881 ttacgtcgct tagcctgtgg cgtgtcgcaa cgggataaca aaacagctac atttgacttc
     2941 agcgcctgta ctttagagtg gcaaaacttc atcgctcagg cgatcgatct gattaacagc
     3001 ccgaaaacga caaaattacc atcaccggta atggctgtac tcgcggcact tgaactgcac
     3061 tgcacgagat ataaagagcg tgcggatgtt atggatcaga ttattcagga aggcggtctg
     3121 gaatatgcta ctgatgtaat aatacatctt caacaaatat ctattgaatg ggattatgtc
     3181 aacaataata ttgttttcct gtcgtctggc atttcacctg attatttgca gcaatattcc
     3241 agctttgaat tacgcctacg taaacattta tcactggcgg aagagtctct ctggcaaaaa
     3301 tgtgcacaaa aacttattgc cgcagttcca catattccag aatggcggca accattaatt
     3361 gctttgttat tgcccgaaaa accagaaatt gcacatgaaa ttgcccagcg tctactgggg
     3421 caaaagaaat taccctcgct ggagtggcta aaaatagtgg cgactgatga gcacattctt
     3481 gcctcattag aaaaatatca cgagccatat gccatttttg atgattacta ttgtggtgcg
     3541 atatggtcag ccaccgtatt acaggagcaa ggtgttgcgg ccctgccccg atttgctccc
     3601 tatgtcgcaa gtgactactg cgccgatgtg ttgcgtcata tcaatcatcc gttcgcatta
     3661 acactgctta tacgtgtagc agggcatact aaacgctgtc acgatcgaat gacgaaagcc
     3721 tgcgctgcgt tcccacacgc agcactggca gcactggcgg aacttctagt gcaaaaagaa
     3781 gagaatagtt ggcgcattat gctaatgacg atgcttatct cgcaaccgac actagcagaa
     3841 caggtcattc cctggctttc gacacccgca gttgcagtgc tgaaatcatg ccagcaacaa
     3901 ctgacacagc cctcaaacca tgccagcgcc gatctactgc cggccatagt agtctcccca
     3961 ccatggcttt cgaaaaagaa aaaatcgccg attccagtgc ttgatttagc accattaggt
     4021 attgagccaa tcagttatct gacagaagaa atcagtaatc aacttttggc gaaatatatc
     4081 tggtattcaa aacacatcac ggttagtcat gaagaaagta ctgccaatct gttggcaagg
     4141 atgggttttc aacgacggat cgctggtaca tatattaaag ctcccgaagc ggtagttgag
     4201 gcatggctaa atgaagatta ttcaacttta ataagtgaat ttaaggtgtt tcattcacct
     4261 accgggcatt attggcattt ggggattttg acaacattgc cgctggagaa agcagtaaga
     4321 gcatggaatg cccttactct atctccgcat accgataccg aatacgccat gttacatttt
     4381 ggactcaaag gattacctgg gttagtaaac tcacttgcac gctatccaca ggaagccttg
     4441 cccataacga attacttcgc agcgagtgag ctggcacctg ccgtcgcccg cgccttcaac
     4501 aaactcaaaa ccctacgaca agacgcccgc acctggctgc tgaaataccc ggaacacgcc
     4561 attaccggcc tgctgtctgc ggcgctcggc aaagccggtg aagcacagga taacgcccgc
     4621 gctgcattgc gtatgcttac cgaaaacggt catcaaccat tactgcaaga aatcgcccgg
     4681 cgttataacc agccggaagt aacagatgcg gtgaacgcta tgcttgcgct cgatccctta
     4741 gataatcacc cgacaaaaat ccccactctt ccggcctttt atcagccatc gatctggacc
     4801 cgcccggtat taaaagcaaa tgcccaatca ctgcccgata gcgcactcca gcacctcggt
     4861 gaaatgctcc gcttccatca ggaggaggcg ctgtatccgg gattattgca ggtgaaagat
     4921 gcctgtaccg ccgactcact ggctgaattt gcctgggatc tgtttaccgc ctggcagacc
     4981 gctggcgcgc cgtcgaaaga gagttgggcg ttcactgcgt taggcgttct cggtaacgat
     5041 gacaccgccc gcaaactgac gccattaata cgcgcctggc ctggtgaatc ccagcataaa
     5101 cgcgccaccg tcgggctgga tatcctcgcc gctatcagta gtgatatcgc cctgatgcag
     5161 cttaacggca ttgcccagaa actgaaattc aaagcattac aggagcgggc gaaagagaaa
     5221 attgccgaca ttgccgaaag tcgcgaactg acggtagcgg agcttgaaga tcggttagca
     5281 ccggctctcg gtctggatga taacggttcg ctgctgctgg attttggccc acggcagttc
     5341 accgtcagct ttgatgaaac cttaaaaccg tttgtgcgcg atgtttccgg cagccgcctg
     5401 aaagacttac ctaaacccaa taaaagcgat gatgaatcac aggcaaacga tgctgttaac
     5461 cgctacaaat tgctgaaaaa agatgcgcgt accgtcgccg cccagcaggt agcaaggctg
     5521 gaatccgcca tgtgcctgcg ccgccgctgg tcgccggaaa actttcagct cttcctggtt
     5581 gagcatccgc tggttcgtca cttaacccgc cgtctgattt ggggcgttta tagcgccgaa
     5641 aaccagctac aggcgtgctt tagggtggcg gaagataaca gctacagcac cgctgacgat
     5701 gatcttttca ccttgcagga aggcgatatc tctgttggca ttcctcacgt tctggaaata
     5761 tcaccgacgg atgctgtcgc ctttggtcag ctttttgccg actacgaact gctaccaccg
     5821 ttccgtcagc ttgaacgtaa cagttacgcc ctgacagaag ccgagcgcaa cgccagcgaa
     5881 ctgacccgct gggcaggcag aaaatgcccg agcggccggg tcatggggct ggcaaataaa
     5941 ggctggataa agggcgaacc acaggatgga ggctggatcg gatggatgat caaacctctc
     6001 ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat gtcaccagcc
     6061 gatcttagcg ctgagcaggt cttaagcaag ctgtggctat gggaaggcaa agcggaaagc
     6121 tatggctggg ggagtaattc aactcaggaa gcgcagttct ccgtgctcga cgacatcacc
     6181 gccagcgaac tcattaacga tattgaagcc ctgtttgaat aaggaaagag catggacaag
     6241 gaattaccgt ggctggcgga taacgcccaa gtggaactga aatataaaaa aggcaaaacg
     6301 ccgctcagcc atcgcagttg gccgggcgaa ccggtgcctg ttatcactga aagcctcatc
     6361 cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat tgtctggcgt
     6421 tatgaaaatt tttcactgga gtggcagtcg gccattacgc aggccatcaa cttgattggc
     6481 gaacacaaac cctcagttcc ggcccggaca atggcggcgc tagcctgtat cgcgcaaaat
     6541 gatagccaac agttgctcga cgaaatcgtc caacaagagg gactggaata tgcgactgag
     6601 gtggtgattg cacgccagtt tattacacga tgttatgaaa gtgatcctct gctagtgaca
     6661 ttgcagtatc agaacgagga ttatggctat ggttatcgat cagaaaccta taacgaattc
     6721 gatctccgac tgcgtaagca tctctctctg gcagaggaaa gcagctggca gcgttgcgcc
     6781 gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt tattgctctg
     6841 atcctcccgg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg cccgtggact
     6901 cattttcatt ctaaggagtg gttaaaagtt gttgctattg accccagagc ggtgagaaaa
     6961 ctcgaacgct actggagcca ggatatattt agcgatcggg aagccagcta catgtcgcat
     7021 gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg actggcagcc
     7081 gttccacgcc tggcaatgta tgcccataaa gaagattgcg gcagtctgct ggtacaaatt
     7141 aaccatccgc aggtcatccg caccctgctg ttagttgctg ataaaaacaa acccagcctg
     7201 caacgtgtag ctaaatacag taaaaatttc ccccatgcga cgcttgccgc actggcagaa
     7261 ctgctggcgc taaaagaacc acctacgcgc cctggttatc caatcatcga agacaaaaag
     7321 ctgcctgcac agcaaaaagc acgtgatgaa tactggcgta cgctgttaca aacgctgatg
     7381 gcatcgcagc cacaactggc agaagaggtg atgccgtggt taagtactca agcccggtca
     7441 gtgctgaata gttatttatc ggcaccgcct aaaacggtta ttgatagcac cgataacagc
     7501 caaatgcctg aaatcctcgt ttcaccaccg tggcgtagta agaaaaaaat gacagttcca
     7561 cgtcttgatt tggcaccgtt cgaattaacg ccgcaagttt actggcaacc aggcgaacga
     7621 gagaggcttg ccgccactga gtctgcccgt tatttcagca cggaatctct tgcggaacgc
     7681 atggaacata aaagtggacg agttgtacta caggagctgg gttttggaga tgatgtatgg
     7741 ctgtttctga attatatact ccccggaaaa ctggatgctg cacgcaattc actcattgtt
     7801 cagtggcatt actaccaggg gcgggttgaa gagatcctga atggctggaa ctccccggaa
     7861 gcacaattag cagaacaggc gcttcgcagc ggtcacgtag aagtgttaat taacatatgg
     7921 gaaaatgaca gctactcacg ctatcgtcgg gaaaagagta tctggaacct gtatttattg
     7981 gcacagttgc cgcgtgagat agctttgact ttctggctac gtatcaatga gaaaaagcat
     8041 ctgttcgcgg gtgaggacta ttttctcagt atcttcggat tggatgctct accaggtctg
     8101 ctgttggctt tttcatatcg tccaaaagaa acatttccgt taattttaaa ttttggcgca
     8161 acagaactgg ccctgcccgt tgcccgcgtc tggcgacgtt tcgcggcgca gcgtgatctg
     8221 gctcgccagt ggattttaca atggccggaa catacggcta ctgcacttat ccctcttgtc
     8281 ttcaccaaat ccagcgataa tagcgaagct gcattacttg ccctacgttt actttacgaa
     8341 cagggacatg gtgaattgct gcaaaccgcg gcaaaccgct ggcagcgtac agatgtatgg
     8401 cctgccctgg agcatttact taaacagagt ccaatagaga tttacccgac acgcattcca
     8461 aaagcccctg atttctggca gccggcaatg tggtccaggc cgcgccttat cacgaataat
     8521 cagcctgtta ccgatgacgc actggaaatt atcggcgaaa tgctgcgctt tacccagggc
     8581 ggacgttttt atagcggact ggaacaactg aaaactttct gccagccgca aacgctggca
     8641 gcttttgcct gggatctttt caccgcgtgg caacaagctg gtgctcccgc aaaagacaac
     8701 tgggcatttc tggcgttaag tctctttggc gacgaaagca cggcacggga tctgacgaca
     8761 cagatccttg cctggccaca ggaaggcaaa tctgcccgtg ccgtcagcgg cctgaacata
     8821 cttactcaga tgaataatga tatggcactg atacagctgc atcatatatc gcaacgggcg
     8881 aaatcccgcc ccttacgtga taacgcggcg gaatttcttc aggtggtcgc agaaaatcga
     8941 gggctaagcc aggaacagtt agcggacaga ttagtcccaa cactgggtct tgatgatccg
     9001 caggcgttga tttttgattt tggtccacgg caatttaccg ttcgcttcga tgaaaacctt
     9061 aacccggtta tctttgatca gcaaaacgtt cgccagaaaa gcgttccccg gttgcgcgcc
     9121 gatgacgatc aactgaaagc acccgaggca ctggcccgac taaaagggct aaaaaaagat
     9181 gccactcagg tgagcaaaaa cctgctcccg cgtcttgaaa ctgccctgcg caccacccga
     9241 cgctggtcgc tggcagattt tcattctctg tttgttaatc atccctttac ccgtctggtt
     9301 actcagcgat taatatgggg tgtttatccg gcaaatgaac cgcgtcgttt actcaacgcc
     9361 tttcgtgtgg ccgctgaggg ggagttctgc aatgagcaag atgagccaat tgacctgcct
     9421 gccgatgctc tgattggcat tgcccacccg ttagaaatgg cagcagaaat gcgcagtgag
     9481 tttgcacagc tttttgccga ttacgaaatt atgccgcctt ttcgccaatt ggcacggcgc
     9541 acagtactgc tcacgcctga cgagtcagcc agtaatagtt tgaatcgctg ggaaggtaaa
     9601 tctgctaccg ttgggcaact tatgggaatg cgatacaaag gctgggagtc aggctatgag
     9661 aatgcatttg tctatgacct gggcgagtac cggctggtcc ttaagttttc atccggtttt
     9721 aaccactaca atgttgatag caaagcgcta atgagcttcc gttctcttca tgtataccgt
     9781 gacaataaat ctgtcacttt tgccgaactt gatgtgtttg atttgagtga agcgttaagc
     9841 gcacccgacg tcattttcca ttaaaacaca gcgggcagtg atgcgactgc ccgttattaa
     9901 cacgacctgc caccgggaaa agcgatgatc gtgcaaaaag agctggttgc tatttacgat
     9961 tatgaggtcc ctgtacctga agatccgttt tccttcagac ttgagatcca taaatgctct
    10021 gaattattta caggttccgt ctaccgactg gagcgattcc ggctacatcc aacatttcat
    10081 caacgtgatc gagaggatgt cgacccgcta ataaatgatg cgttgattta tataagagat
    10141 gagtttattg atgagcgaaa attacggggc gaatcaccag aaacggtaat agccattttt
    10201 aatcgcgaac tacagaatat attcaaccaa gaaatagaaa aatagcttta ttaaaggggg
    10261 aaacggtatt cccccttaga tgcgtttcaa tttgcccatt acttcaacaa atcaatcaac
    10321 cgtaacagag ccactaatag ctccagtacg gcaatgagca cggttaactt ttgtgtcagc
    10381 gtgtccatcc gctctcctta ttaaggagcg caacaccagc accttaccct tacactgcct
    10441 gtcgatactg gtttacgtat gttggaccac gatgttgcac tccggatcag ggcaccacct
    10501 gcagcaccac ccgtccggta gccaggactt aaaattggtc cgatccggcg caacgtccca
    10561 accgtctgga ttataaatct cattatctta attgcaacgg ggtccaccga tggttataat
    10621 tcgttcgttg gaacacgatg ttgcacaggc tgcggtgttg ggcctgaaaa tagtaaataa
    10681 aagaacctgc ctcaccagca ggttttttta tttactgtga tctgctttcc agatattttt
    10741 cgctcaaaca actaatgcgc caaacattta ttgcgcgtaa aatatcgttt atttcattaa
    10801 tacatttcag ggatgaatat atgtcaccgc agaataatca tctgcagcgt ccgcctgctg
    10861 ctgtgttata cgccgatgaa ctggcaaaat taaaacaaaa tgataacgca ccttgcccgc
    10921 ccggttggca gttaagtttg cctgcggccc gtgcttttat ccttggcgat agcgcgcaaa
    10981 atattagccg taaagtggtg attagtccct ccgctgtcga acgtatgtta gtcactcttg
    11041 ccaccgggcg cggtttgatg ttggtggggg aaccgggcac cgcaaaatct ctcctgtctg
    11101 aattactggc aaccgctatt agcggcgatg ccgggttaac catccagggc ggcgcatcta
    11161 ctactgaaga tcaaatggaa ctacgccctg cttatcaacc atggtccgtc tacggaagca
    11221 ctggtccctg ccccgcttta ccagggaatg cgcgacggca aaatcgtccg ttttgaagag
    11281 ataacgcgta caccgctgga agtacaagac tgtctgttag gtatgctttc tgacagggtg
    11341 atgacggtgc cggaactcac cggtgaagcc agtcagctct atgcgcggga aggctttaat
    11401 attattgcca ctgccaatac ccgcgatcgc ggcgtcaatg agatgagtgc cgcgctaaag
    11461 cgtcgcttcg attttgaaac cgtctttccg attatggatt tcgcccagga gctggaactg
    11521 gtcgccagtg cttcggcgcg tttgctggcc catagcggta ttccgcataa agtgcctgac
    11581 gcggtactgg agttactggt ccgcaccttc cgcgatctgc gtgccaacgg cgaaaagaaa
    11641 acgtcaatgg atactctgac ggcaattata tccaccgctg aagccgtgaa cgtggcccac
    11701 gcagtgggcg tccgcgcctg gtttttagcg aatcgcgcag gtgagccagc cgatctggtg
    11761 gactgtattg ccggaaccat cgttaaagat aacgaagaag atcgcgcgcg tctgcgccgt
    11821 tacttcgaac agcgtgtcgc cacacataaa gaagctcact ggcaggctta ttatcaagcc
    11881 cgccaccgcc tgccgtgagg aaagatgcat gagcgagccg ttaattgtcg gcatccggca
    11941 tcatagtccg gcctgcgccc ggctggtgaa atcgttaatc gaaagccagc ggccacgata
    12001 cgtgttgatt gaaggcccgg ctgattttaa tgaccgggta gacgaactgt ttttagccca
    12061 ccagcttccg gtagctattt acagttattg ccagtatcag gacggtgcag cccccgggcg
    12121 tggtgcctgg acgccatttg ctgaattttc gccggagtgg caggcgctac aagccgcacg
    12181 tcgtattcag gcacaaactt acttcatcga tttgccttgc tgggcgcaga gtgaagaaga
    12241 agacgaatca cccgatacgc aagatgacag ccagacctta ctgctgcgtg ccacccgcat
    12301 ggataacagc gacaccctgt gggatcactt gttcgaagat gaaagccagc aaactgcatt
    12361 accctctgcg ctggcgcact attttgcaca actgcggggc gattcccccg gagatgccct
    12421 caatcgtcag cgcgaagcct ttatggcccg ctggattgga tgggcgatgc agcaaaacaa
    12481 tggcgacgtg ttagtcgtct gcggcggctg gcatgctccg gcactggcaa agatgtggcg
    12541 cgaatacacg caggaaatta acacgccgga attgccctag ctggcagatg ccgttacagg
    12601 ttgttatctc accccctaca gtgaaaagcg ccttgatgtg ctggcaggat acctttctgg
    12661 tatgcctgcc ccggtctggc aaaactggtg ctggcagtgg ggcctacagc aggccggtga
    12721 acaactgcta aaaactatcc ttacccgttt gcgccagcac aaattgcccg cttctaccgc
    12781 tgatatggct gccgctcatc tgcatgcgat ggcgctggca cagttgcgcg gtcatacact
    12841 accgttacgc actgactggc tggatgccat agcaggctcg ctgattaaag aagccttgaa
    12901 cgcgccgttg ccgtggagct atcgcggcgt tattcatccc gataccgatc cgattctgct
    12961 aacgttgata gacacattag cgggtgacgg attcggtaaa cttgcccctt ctacgccaca
    13021 accgcctctg ccaaaagatg tcacctgcga actggaacgt accggaatct ctcttccggc
    13081 ggagcttacc ttaaatcgct ttacccccga tggactggcg caaagtcagg tgttacatcg
    13141 gttggcaata ctggagatcc caggggttgt gcgccagcag ggaagtacac tgtcacttgc
    13201 aggcaacggt gaagaatgct ggaaattaac ccgcccgctt agccagcatg cggcattgat
    13261 tgaggccgcc tgctttggtg ccacactcca ggaagccgca cgccataaat tagaagccga
    13321 tatgctggac gcgggtggaa tcagcatcat caccacatgt cttagccagg cggcgttagc
    13381 gggtctggcg tctttcagtc aacaattact ggagcaactt acattattaa tcgcccagga
    13441 aaatcaattt gccgaaatgg gccaggcgct ggaagtgcta tatgccttat ggcggttaga
    13501 tgaaatcagc ggtatgcaag gcgcgcagat attgcagacg acgttatgcg cggctatcga
    13561 tcgcacactc tggctgtgtg aatccaacgg caaaccggag gaaaaggagt ttcacgctca
    13621 cctgcatagc tggcaggcgc tttgccatat cctgcgcgat ctacatagcg gcgttaattt
    13681 acccggcgtt tcgctttctg cggcggtagc cttactggag cgccgcagtc aggcgattca
    13741 tgccccggcg ctggatcgcg gcgcggctct tggcgcacta atgcgtctgg aacatcccaa
    13801 cgccagtgcc gaagcggcgc tgacaatgct ggcgcaatta tccccggcac aatccggcga
    13861 agcgctgcac ggtttgctgg cgctggcccg ccatcaactg gcctgtcagc cggcatttat
    13921 cgccggtttc agtagtcatt taaatcaact gagtaacgac gattttatta acgccctgcc
    13981 cgatttacgc gcggcgatgg cctggctacc accacgagaa cgcgggacgc tggcgcatca
    14041 ggtgtttgag cattatcaac tggcgcaact tcccgtttcg gcactgcaaa tgccgttgca
    14101 ttgtccaccg caagccattg cacatcatca acaactcgaa cagcaggcac tggcatcgct
    14161 gcaacactgg ggagttttcc atgtctgaac tgaacgatct tctgaccacc cgtgagctac
    14221 aacgctggcg attaattctt ggcgaagcgg cagaaacgac cctttgtggg ctggatgaca
    14281 atgcccggca gatagaccac gcgctggagt ggctgtatgg gcgcgatcct gaacggctcc
    14341 agcgtggtga acgctccggt ggattaggtt gctcaaatct caccacccct gagtggatca
    14401 acagtattca cacgctgttt ccgcaacagg tgatcgagcg gctggaaagc gatgccgtgc
    14461 tgcgctatgg cattgaagat gtggtgacga atctcgacgt actggaacgt atgcagcctt
    14521 ctgaaagcct gctacgcgcc gttttgcaca ccaaacatct gatgaatccc gaagtgctgg
    14581 ctgccgctcg ccggatagtg cgccaggttg ttgaagagat tatggcgcga ctggcaaagg
    14641 aagttcgcca ggctttttct ggtgtccgcg atcgccgccg tcgctcattt attccactgg
    14701 cgcgtaattt cgatttcaaa agtacgctgc gtgccaacct gcaacactgg catccgcaac
    14761 acggcaagtt gtatatcgaa tccccccgct ttaacagccg cattaagcgc catagcgatc
    14821 aatggcaact ggtcttactg gttgatcaaa gcggatcgat ggttgactcg gtgatccact
    14881 cagcggtgat ggcggcctgt ttatggcagt tacccggtat tcgtacccat ctggtggcgt
    14941 ttgacaccag cgtggttgat ctcacggcag acgttgccga tccggtagag ttattaatga
    15001 aagtgcaatt aggcggcgga accaatatcg ccagtgccat ggaatatggt cggcaactta
    15061 ttgaacaacc agcgaaaagc gtcattatcc tcgtgagcga tttttacgaa gggggttcat
    15121 catcattact gacgcatcag gtgaaaaagt gtgtccagag cggcatcaaa gtgctgggac
    15181 tggcggcgct cgatagcacc gcaacacctt gctatgacca cgatacggcc caggcgctgg
    15241 ttaatgtcgg cgcacaaata gccgccatga caccgggcga gctggcatca tggcttgcgg
    15301 agaatcttca gtcatgaatt cactacgtcc ggaattatta gaactgacac cgcaggccct
    15361 gacagcgtta agcaatgccg gttttgttaa gcgcagtctt aaggaactgg aaaatggcaa
    15421 cgtcccggag atcagccatg agaacggcgc tttaatcgcc accttcagtg acggtgtccg
    15481 tacccagctg gcgaacggtc aggcactgaa agaggctcag tgcagctgcg gggccagcgg
    15541 catgtgccgc catcgcgtga tgctggtttt aagttatcag cgactttgcg ccaccaccca
    15601 gactacggaa aaagaagaag agtgggatcc ggcaatctgg ctggaagaac tggctaccct
    15661 gcccgatgcc acccgcaaac gcgcacaggc actggtcgct aaaggcatca ccattgagtt
    15721 gttctgtacg ccgagcgaaa ttccctctgc ccgcttaccg atgagcgatg tacgttttta
    15781 ttcccgcagc agcattcgtt tcgctcgctg tgattgtatt gaaggaacac tttgcgaaca
    15841 tgtcgtactg gcagtacagg cctttgtcca ggccaaagcg cagcaagcgg aatttactca
    15901 tttaatctgg cagatgcgca gcgaacacgt cacatcatct gacgatccgt ttgccaacga
    15961 tgagggggac gcgtgtcgtc aatatgttca gcaattaagc caggcgttat ggcttggcgg
    16021 catcagccag ccgcttattc attacgaggc tgctttcagc cgcgcgcagc aagcggcgga
    16081 acgctgcaac tggcgctggg tgagtgaatc gctacggcaa ctgcgcgcca gcgttgatgc
    16141 cttccacgcc cgcgccagcc actatcatgc cggagaatgc ttacgtcagc ttgcggcatt
    16201 aaacaatcga ttaaattgtg cacaagagat ggcccggcgc gacagtgttg gtgaagttcc
    16261 tcccatgccg tggcgcacgg tcgttggcgc tggcattgcc ggagaagcaa agcttgatca
    16321 tctgcggctg gtgtctttag gtatgcgttg ctggcaggat attgagcagt atggtttacg
    16381 catctggttt accgatcccg acaccggcag tattttgcat ctttcgcgta gttggccgag
    16441 aagcgaacag gaaaactcac cggcagcgac gcgtcggctg tttagttttc aggctggcgc
    16501 actggctggt gggcaaattg tttcacaagc agcaaaacgc agtgccgatg gcgagctgct
    16561 gttagctacc cgcaaccgct tgagtagtgt tgttccactg tcgccagacg cctggcaaat
    16621 gttgagtgcg ccgttgcgcc agccgggcat tgtggctttg cgtgaatatt tacgccagcg
    16681 tccccccgcc tgcatacggc ctcttaatca ggtggataac ttatttattc tgccagtcgc
    16741 tgagtgtatt tcactcggtt gggatagcag ccgccagacg ctggacgcgc aggtaatcag
    16801 cggcgaaggt gaagataatc tgctgacgtt atcccttccg gcatctgcct gttctccttt
    16861 tgccgttgaa cgcatggcgg cgcttttgca acaaacagac gaccccgtga gtctggtttc
    16921 tggctttgtc agctttgtag aggggcaatt gacactggaa ccacgggtaa tgatgaccaa
    16981 aacccgcgcc tgggcgctgg acgcagaaac tgcgcctgtg gcaccgctac cttctgccag
    17041 cgttttgcct gtgccgtcta ccgctcatca gttgctgatg cgctgccagg cgttacttat
    17101 tcaactgctc cataacggct ggcgctatca ggaacagagt gctattggtc aggcagagtt
    17161 gctggcgaat gacctcaccg cggtggtttt ttatcggctg gcacatgtgt tggcacaatt
    17221 tcgtaataca gaaagcgagg cacgggtaga agcaatgaat aacggtgttt tactttgcga
    17281 acaattattc cccatgcttc agcaacaagg atgaaatagt gctttttact aagagttcta
    17341 ctccagttcc ggactgctca cgtcacggta ttaggcatat cctatatagc tcctggtgag
    17401 agtcaccagt ttcttgatta aataaaatgg agttttacat gaaggctttc aataagctgt
    17461 tttccctcgt tgttgcatct gttctggttt tctctcttgc tggctgcggt gacaaagaag
    17521 aatcgaaaaa attcagcgcc aatctgaacg gtactgaaat tgccattacc tatgtctaca
    17581 aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat tcaatttgcc tccattggtg
    17641 caaccaccaa agaagatgct gccaagacac ttgagccgtt aagcgccaaa tataaaaaca
    17701 tcgcgggtgt tgaagaaaaa ttaacctata ccgataccta cgcgcaggaa aacgtgacta
    17761 tcgatatgga aaaagtggat ttcaaagccc tgcagggtat ttcaggaatc aacgtttctg
    17821 ctgaagatgc caaaaaaggt atcactatg
//
LOCUS       DAOTRK010000099        17786 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.099, whole genome shotgun sequence.
ACCESSION   DAOTRK010000099 DAOTRK010000000
VERSION     DAOTRK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.099"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1351
                     /locus_tag="QIE41_003163"
     CDS             <1..1351
                     /locus_tag="QIE41_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDS3532018.1"
                     /translation="ISVKFYRNEEIECACDFLMDKDAQGYTDLSDLDLTSCHFKGDVI
                     SKVSFLSSNLQHVTFECKEIGDCNFTTATVDNVIFKCRRLHNVIFIKASGEYVDFSQS
                     ILDTVDFSRSQLTHSNFRECQIRNSKFNNCYLYASHFTRAEFLSDKEISFIKSNLTAV
                     VFDHVRISTGNFKDSVTQRMVLSIDYSDIFGNEDLDDYINNIIKMIDTLPDDPAILKS
                     VLAVKLVMQLKILNIVNKNFIENMKKIFSHCPYIKDPIIRSYIHPDEDNKFDNFMRQN
                     RFSKMHFDTQQMIDFINRFNMNKWLIDQNNNFFIQLIDQALRSTNDTIKENAWHLYKE
                     WIRSDDVSPLFIEIEDNLRTFNTNELTRNDNIFILFSSVYDGPVMVVSSQRLHDMLNP
                     TKDTNWNSTYIYKSRHEMLPVNLTPETLFGTKSQDKHALFPIFTASWRANRIKNKGI"
     gene            1574..2071
                     /gene="ubiC"
                     /locus_tag="QIE41_003164"
     CDS             1574..2071
                     /gene="ubiC"
                     /locus_tag="QIE41_003164"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDS3532019.1"
                     /translation="MSHPALTQLRALRYFTEIPALDTQLRDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            2084..2956
                     /gene="ubiA"
                     /locus_tag="QIE41_003165"
     CDS             2084..2956
                     /gene="ubiA"
                     /locus_tag="QIE41_003165"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDS3532020.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(3111..5534)
                     /gene="plsB"
                     /locus_tag="QIE41_003166"
     CDS             complement(3111..5534)
                     /gene="plsB"
                     /locus_tag="QIE41_003166"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDS3532021.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5705..6073
                     /gene="dgkA"
                     /locus_tag="QIE41_003167"
     CDS             5705..6073
                     /gene="dgkA"
                     /locus_tag="QIE41_003167"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDS3532022.1"
                     /translation="MANNTTGLTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6183..6791
                     /gene="lexA"
                     /locus_tag="QIE41_003168"
     CDS             6183..6791
                     /gene="lexA"
                     /locus_tag="QIE41_003168"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDS3532023.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6864..8189
                     /gene="dinF"
                     /locus_tag="QIE41_003169"
     CDS             6864..8189
                     /gene="dinF"
                     /locus_tag="QIE41_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDS3532024.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            8305..8514
                     /locus_tag="QIE41_003170"
     CDS             8305..8514
                     /locus_tag="QIE41_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDS3532025.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(8556..9071)
                     /gene="zur"
                     /locus_tag="QIE41_003171"
     CDS             complement(8556..9071)
                     /gene="zur"
                     /locus_tag="QIE41_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDS3532026.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKSR"
     gene            9389..10351
                     /locus_tag="QIE41_003172"
     CDS             9389..10351
                     /locus_tag="QIE41_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDS3532027.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKGKVNSIYGKSIDYSAM
                     RHRDIIIAKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEGLNDRE
                     FIASVVDRAIFHFAINSICNPEDNKDATPIERCTFDVETKNGLPSTVQLFYEESKDNE
                     PLANIHLQAIGSGFLTFVNACQEYDDNSLKLFASLLISLSYSSAYVDLAGAERVKINE
                     YNENYLTAQFEELSQRDMKKYLGEMKRLADGGEMNFDDYLDKMSLLVNEGKLEPDILS
                     KMRDAAPKLIDFAKSFDPNSKEKIKILTDTSKLIYDLFGVKSEK"
     gene            10714..11751
                     /gene="dusA"
                     /locus_tag="QIE41_003173"
     CDS             10714..11751
                     /gene="dusA"
                     /locus_tag="QIE41_003173"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDS3532028.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            11885..12127
                     /gene="pspG"
                     /locus_tag="QIE41_003174"
     CDS             11885..12127
                     /gene="pspG"
                     /locus_tag="QIE41_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDS3532029.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(12293..13276)
                     /locus_tag="QIE41_003175"
     CDS             complement(12293..13276)
                     /locus_tag="QIE41_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDS3532030.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            13359..14774
                     /gene="dnaB"
                     /locus_tag="QIE41_003176"
     CDS             13359..14774
                     /gene="dnaB"
                     /locus_tag="QIE41_003176"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDS3532031.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            14827..15906
                     /gene="alr"
                     /locus_tag="QIE41_003177"
     CDS             14827..15906
                     /gene="alr"
                     /locus_tag="QIE41_003177"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDS3532032.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGLRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            16159..17352
                     /gene="tyrB"
                     /locus_tag="QIE41_003178"
     CDS             16159..17352
                     /gene="tyrB"
                     /locus_tag="QIE41_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDS3532033.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGIYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(17574..>17786)
                     /locus_tag="QIE41_003179"
     CDS             complement(17574..>17786)
                     /locus_tag="QIE41_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532034.1"
                     /translation="KGDYSHYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQ
                     KVTGNKAKHILEDIEIFYRNKPDTYY"
BASE COUNT         4362 a         4124 c         4597 g         4703 t
ORIGIN      
        1 tataagcgta aaattctata gaaatgaaga aatagaatgt gcttgtgatt ttctgatgga
       61 taaagatgcg caggggtata ctgacctgtc tgatttggat ttaacaagtt gtcattttaa
      121 aggtgacgtt atttcgaagg tgtctttttt atcgtcaaat ctacaacatg taacattcga
      181 atgtaaagaa ataggggatt gcaattttac tactgcaaca gttgataatg tcatatttaa
      241 atgtcgacgt ttacacaatg tgatttttat caaagcgagt ggcgaatatg tcgattttag
      301 ccaaagtatt cttgatacag ttgacttctc gcggagccag cttactcata gtaattttcg
      361 tgaatgtcag attagaaatt caaagttcaa taattgttat ctttatgctt cgcacttcac
      421 cagagcagaa tttctttctg acaaagaaat atcatttatt aaatcgaatt tgacagctgt
      481 cgtgtttgat catgtgcgaa tatcgacagg gaattttaaa gacagcgtta cacaacgaat
      541 ggtattatct attgattact cagatatatt tggaaatgaa gatctcgatg actatatcaa
      601 taatattata aaaatgattg atacattgcc agatgatcca gcgatattga aatccgttct
      661 ggcagtaaaa ttggtgatgc aattaaaaat tcttaatatt gttaataaaa actttattga
      721 gaatatgaag aaaatattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      781 tatccatcct gatgaagata acaagttcga taattttatg cgtcaaaatc gattcagtaa
      841 gatgcatttc gatacccaac agatgatcga ttttattaac agatttaata tgaataaatg
      901 gctgattgat caaaataaca atttttttat ccaacttatc gatcaggctc tacgatcaac
      961 gaatgatacg atcaaagaaa atgcctggca tctttataaa gagtggattc gtagtgatga
     1021 tgtttcacct ttatttatag aaattgaaga taatttaaga acctttaaca cgaatgaatt
     1081 aacacgaaac gataatatct ttatcctgtt ctcctctgtc tatgatgggc cagttatggt
     1141 ggtaagctcc cagcgcttac atgatatgtt gaatcctaca aaagatacca attggaattc
     1201 cacgtatatc tacaaatcca gacatgagat gttgcctgtt aatcttactc cggaaacact
     1261 tttcggcacc aaatctcagg ataaacatgc tcttttccca atttttactg cgagttggcg
     1321 agctaatcgt ataaagaata aaggtattta agtaaaggaa aacatcatcg ttcctggcat
     1381 cctgggcggt gatgtcccta tggttgccct cgccagcacg ggcatcggca aagcgtaagg
     1441 ttcaacagcg ttttaccact tcatgcgatt gttgcgtttt tgttgcgcat tagatcactt
     1501 aatttgcttt acatctcccg taaacacttt tctgcgatac aatgccttta cgttatgtaa
     1561 cggagagttc ggcatgtcac accccgcgtt aacgcaactg cgtgcgctgc gctattttac
     1621 agagatccct gccctggata cgcaactgcg cgactggctg ttgctggaag attccatgac
     1681 aaaacgtttt gaacagcagg gaaaaacggt gagcgtgacg atgatccgcg aagggtttgt
     1741 cgagcagaat gaaatccccg aagaactgcc gctgctgccg aaagagtctc gttactggtt
     1801 acgtgaaatt ttgttatgtg ccgatggtga accgtggctt gccggtcgta ccgtcgttcc
     1861 tgtgtcaacg ttaagcgggc cggagctggc gttacaaaaa ttgggtaaaa cgccgttagg
     1921 acgctatctg ttcacatcat cgacattaac ccgggacttt attgagatag gccgtgatgc
     1981 cgggctgtgg gggcgacgtt cccgcctgcg attaagcggt aaaccgctgt tgctaacaga
     2041 actgttttta ccggcgtcac cgttgtacta agaggaaaaa aatatggagt ggagtctgac
     2101 gcagaataag ctgctggcgt ttcatcgctt aatgcgtacg gataagccaa ttggcgcgtt
     2161 actgctgctc tggccaacat tatgggcgtt gtgggtggcg acaccgggcg ttccccagct
     2221 ctggatcctg gcggtgtttg tcgcgggtgt ctggctgatg cgcgctgccg gatgtgtggt
     2281 gaatgattat gctgaccgca agtttgatgg tcatgttaag cgcacggcga accgaccgct
     2341 tcccagcggc gcggtaacgg agaaagaggc gcgcgcgctg tttgtcgtgc tggtactgat
     2401 ttcgttttta ctggtgctga cgctgaatac gatgaccatt ctgttgtcga ttgccgcgct
     2461 ggcgctggcg tgggtgtacc cgtttatgaa gcggtatacc catctaccgc aagtggtgct
     2521 gggcgcggcg tttggctggt cgattccaat ggcttttgct gctgtgagtg agtcggtgcc
     2581 attgagttgc tggttaatgt tcctcgccaa tattctctgg gcggtggctt acgacacgca
     2641 gtatgcgatg gttgaccgcg atgatgatgt gaagattggc attaaatcca ccgcaatcct
     2701 gttcggccaa tacgataaat tgattattgg tattttgcag attggcgtac tggcgctgat
     2761 ggcgatcatc ggtgagttaa atggcttagg ctggggatat tactggtcaa ttctggtggc
     2821 tggcgcgctg tttgtttatc aacaaaaact gattgccaac cgcgagcgtg aagcctgctt
     2881 taaagcattt atgaataata actatgttgg tctggtactg tttttagggc tggcaatgag
     2941 ttactggcat ttctgatgat gtaaaaaagc cggatgatca tccggcttcc ttctgggttg
     3001 cctgatgcgc tgcgcttctc aggcctacac aacatatcgc aatttattga atttgcagat
     3061 catggtaggc cgaataaggc gtttgcgccg catccggcaa ttctccctga ttacccttcg
     3121 ccctgcgtcg cactctcaat cgtcaaacgc acgtctgatg taatcaactc cgccagcaac
     3181 tgatacacct tcatcgtttc tgccggttcg gcgtcgccgc tatcgctgat atacccttca
     3241 tcacgcagtg ttagcaccag agaactgaac accgccttgt cgaagaactc cggcgcgttg
     3301 ataccgtgca gcacggagag acgttgcgcg acggtgcggc tctctttctc cagcgtaccg
     3361 cggttgatcg acgggttggc gctcaacagc cagaaggtaa tggcataacg ttgcagcgtt
     3421 tcgcgcgcgc ctgcggccag cagctgtagc gtgcgagaat gcgccgggtt gatatgcaac
     3481 tcatcatctt gcagggtaat cagcccctga cgttgcatct catttgccag tgcatcaata
     3541 acgtccggca actcgtcgcg atcccagcgc aggaacagct ccgctttcag cattgggtaa
     3601 agcacattga cgtgctccat caatacgtcg cgggagatgt ggcgatgctg ggtgacaatt
     3661 gccgccatca gcgaaggcag caccaacata tgcgcaatgt tgttgcgata gtaggtcatc
     3721 agcaccgctt gctcgcgcgg cagaatgatg atgtcgccga tagtgtcttt ctcaacttca
     3781 aacttgttca tttgcagcgc gtgatcgata agctcgctgg cgctggctga aggaacggta
     3841 gagtccgtgg agtaaggcac gttgcgcatc agatccaggt agcagttgag ttgctcggtt
     3901 aactgctcgc gggtgagtga gcgctgacgt gacgccagaa gcgcagtaca gcacaggttc
     3961 atggcgtttg ccgcgcctgc gttgttaatg cgtaccatca gatcggcagc aatattattg
     4021 accgtcggcg ttaaccaggc cggacgcacc gcttcgatgg gatcgataga ttcacgccag
     4081 tctggtacgt gctggttaag gtaggtcatc aacggcattg gttcaccgaa gttgacgtaa
     4141 ccctgaccga gattacgcag cttgcttaaa ccgcgcagca tctgcggcag gctctctttc
     4201 tctttcgttg caccgcgcag ttctttggcg taagtaccca cttccatgac gtgctcataa
     4261 ccgatgtaga tcggaatcag cgtaatcgga cgcgtgccgc cacgcagcat cgcctgaatg
     4321 gtcatcgata gcgttccggt tttcggatcc agcaaacgcc ccgtacggga acgaccgcct
     4381 tccacgaaat actcgacgga ataaccacgg ctgaacagtt cgccgagata ctcacggaaa
     4441 acggtggaat agagcttatt gcctttaaac gtacggcgaa taaagaacgc ccccagacgg
     4501 cggaaaatcg gcccggcagg ccagaaattc aggttgatcc cagcggcgat atgcggcggc
     4561 accagcccct ggtgatacag cacgtaagaa agcagcaggt agtccatgtg actgcggtgg
     4621 caaggcacat acaccagctc atgaccgtcg tgggccaact ggcgaacgcg ctcggcgtta
     4681 tggacgttga taccctggta aagtcggttc caggtgaagc ccagaatacg gtcagtcagg
     4741 cgaatcatct cgtaagagaa attcgccgca atctcttcca tcagtgcaat cgcgttctgc
     4801 tgcgcttttt catgggagat ttttttgctg cgcgcttcat cttctaccgc tttcgcaatg
     4861 gcgcgggagg cgagcagctt attaaacaga tcctgacgag caggcaaacg tgggcctacg
     4921 gcagccagac gttgacgggc aaagtgcata cgcgccacgc gcgccagttt ctgcgcgata
     4981 gttttatccg tgccgtgttc atccgccata cggcgcagcg aaactgacgg tgagaaacgc
     5041 acaaaactgt cgcgaccgag ccacagtaca gcgaaaaatt tctgtacgcc gttaagcata
     5101 cgcagcggcg ggttcacttc gcctttttca cgccccggcg cgcgaccaaa catcaccgac
     5161 actggcacca tctgcacatc cagatttggg ttgctacggt gcaaatcgag atagtcgtgg
     5221 aacagcttaa tagactcttc tttcggcgtg taataggtga atacacgcgg cccgccgtga
     5281 atgaacacat agcgcggcag tagcgtgccg tcgatttcca gcggctctaa cgggtcaggc
     5341 aggtcatgtg ccagacactg ggcgcgcaac gtcagcaagt ctgctttcga gttgtacggt
     5401 aaaacgtaca taattggacg agaggtatcc agccccagtt ccggggcagg atctgccgga
     5461 atagacttgc tttttaccag gatgcttaat ggtaaattca gtaatttgta gtaaattcgt
     5521 ggccagccgg acataaacga tgtaaagcct ctggttaata atgcaaatgc gcggcaagga
     5581 tagcagaaag tcatgggaaa ttctgtggta tccgctcatg tttcgcgcgg cgttacgcaa
     5641 acccgaatca tcggatttaa cggtacactg atattgacgc tcataatgta aaaaggttct
     5701 ttcaatggcc aataatacca ctggactcac ccgaattatc aaagctgctg gctattcctg
     5761 gaaaggttta cgcgctgcat ggatcaacga agcggcattc cgtcaggaag gcgtagcggt
     5821 attgttggcg gtggtcatcg cctgctggct ggatgtggac gcaattaccc gcgtgctgct
     5881 tatcagctcc gtgatgctgg tgatgattgt ggaaatcctc aatagcgcca tcgaagcagt
     5941 ggttgaccga attggctctg aataccatga gctatccgga cgcgctaaag atatgggatc
     6001 cgccgcggtg ctgattgcga ttatcgtcgc cgtgattacc tggtgcatcc tgttatggtc
     6061 gcattttgga taacccttcc agatttcgat aaatctctgg tttattgtgc agtttatggt
     6121 tccaaaatcg ccttttgctg tatatactca cagcataact gtatatacac ccagggggcg
     6181 gaatgaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt gatcacatca
     6241 gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc
     6301 caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt
     6361 ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg ccgctggtag
     6421 gtcgtgtggc tgccggtgaa ccgcttctgg cgcaacagca tattgaaggt cattatcagg
     6481 tcgatccttc cttattcaag ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga
     6541 tgaaagatat cggcattatg gatggcgact tgctggcagt gcataaaact caggatgtac
     6601 gtaacggtca ggtcgttgtc gcacgtattg atgacgaagt taccgttaag cgcctgaaaa
     6661 aacagggcaa taaagtcgaa ctgttgccag aaaatagcga gtttaaacca attgtcgttg
     6721 accttcgtca gcagagcttc accattgaag ggctggcggt tggggttatt cgcaacggcg
     6781 actggctgta acatatctct gagaccgcga tgccgccagg cgtcgcggtt tgtttttcat
     6841 ctctcttcat caggcttgtc tgcatggcat tcctcacttc atctgataaa gcactctggc
     6901 atctcgcctt acccatgatt ttctccaata tcaccgttcc gttgctggga ctggtcgata
     6961 cggcggtaat tggtcatctt gatagcccgg tttatttggg cggcgtggcg gttggtgcaa
     7021 cggcgaccag ctttctcttt atgctgttgc tgtttttgcg catgagcact accgggctga
     7081 ctgcgcaggc ttatggtgcc aaaaatcctc aggcattagc ccgtgcgctg gtgcaaccgt
     7141 tgctgttggc gttgggggcc ggggcgttaa ttgcgctgct gcgtacgccg attatcgatc
     7201 tggcgctgca tattgttggc ggcagcgaag cggtgctaga acaggcgcgg cgctttcttg
     7261 aaatccgctg gttaagcgca ccggcgtcgc tggcgaatct ggtattactt ggttggttac
     7321 tcggcgtgca atatgcccgt gcaccagtaa ttttgttagt ggtcggcaat atcctcaaca
     7381 ttgtgctgga tgtctggctg gtgatggggc tgcatatgaa cgtgcagggc gcggcgctgg
     7441 cgacggttat tgcggaatat gcaacattgc tgattggtct gctaatggtg cgtaaaatcc
     7501 tcaaacttcg cggaatatcc ggcgaaatgc tgaaaactgc ctggcgagga aacttccgtc
     7561 gcttgctggc gcttaaccgc gatatcatgc tgcgctcgct gttgttgcaa ctctgtttcg
     7621 gcgcgatcac cgtacttggc gcgcgactgg ggagtgacat tatcgctgtt aacgcggttc
     7681 tgatgacgtt actcaccttt accgcctatg cgctggatgg ttttgcctac gcggttgaag
     7741 cacattctgg tcaggcgtac ggtgcgcgcg acggtagcca gttactggat gtctggcggg
     7801 cagcgtgccg ccagtcgggg attgtagcgt tactgttttc ggtggtttat ttgctggcag
     7861 gggaacacat cattgcgtta ctgacgtcgt taacccagat tcagcagctg gccgaccgct
     7921 atcttatctg gcaggtgatt ttgccgttgg ttggcgtctg gtgttatctg ctggacggca
     7981 tgtttatagg cgcaacgcgt gccgccgaaa tgcgtaacag tatggcggtg gccgccgcag
     8041 gttttgcgct gacgctcctt acgctgccgt ggctgggtaa tcatggtttg tggctggcat
     8101 taaccgtctt tctggctttg cgcgggcttt ctctggcggc tatctggcgg cgtcactggc
     8161 gcaatggtac ctggtttgcc gcaacgtgac ggttaaaaat tctgaataaa taatcctaag
     8221 ccaaattgct gactacactt aatctcacgt tcagaagaaa agtgaacgta ctctcattca
     8281 caacctaacg atgaggtctt gattatgaat aaagatgaag ccggcggtaa ctggaaacag
     8341 tttaaaggta aagtgaaaga gcaatggggc aaactgaccg atgatgatat gacgatcatt
     8401 gaaggtaaac gtgatcaact ggtcggtaaa atccaggaac gttatggtta tcagaaagat
     8461 caggcagaaa aagaggtcgt ggattgggaa acccgcaatg agtatcgctg gtaattaatc
     8521 cctcctgccc gacgtgtaca aggatgtacg ccctcttaac gtgatttctt tttcacctgt
     8581 acagagtgat catgctggca ctgttcagga tgacgacacg cttccacttc cacgcatgcc
     8641 gcacagagtc catgtgcttc aatcacatta tgccgcaggg caaaccccat tttggccgcc
     8701 agcgtatgca taatgtcttc cacgccttct gcacattctt ctttcaccgc gccgcagcga
     8761 tcgcaaataa acatggctga agtatgggtg ggctggtcga acagatgaca gagcacatag
     8821 ctgttggtgg attccacctt atgcacaaaa ccttgctcaa gaagaaaatc cagcgcgcga
     8881 taaaccgttg gcggcttggc ttgcggctca gcttcgcgca gcaaatcaag cagatcataa
     8941 gcgctgatag cgccatcctg cagactcatc aggcgcaaca cttccaggcg ctgtggggtc
     9001 aggcgcacat tacgctgcgc gcagattttt tcagcctgcg ctaataactc ctgcgttgtg
     9061 gtcttttcca tcgggcacct caaagtgggg gttaagaatg tccttacttt accatgttcc
     9121 aggaaaaaca ccgagaaccc ttattgttgc cgtaatgttg attttctgtt ttgtacgtaa
     9181 ggtgttgttt tgcctggtag taccattatc aacacgataa taattaataa ttcattttta
     9241 aataaattgc ttcaagttaa atcgctattt atttagctgg gtttgattgc atacctgtac
     9301 ttcagagatt atgagcgatt tttgatagtg agcagatggc aaaagaatta taaggaggac
     9361 acggtacgac aaaaaaggta gatgaatcat gttgaagata atacccggtg caactggata
     9421 ctttaataaa acactcaatt caaatcaatt cgataatgaa gatgcaatca aaggtaaagt
     9481 aaatagtata tatggtaaat ctattgatta ttcagcgatg cgtcatcggg atataattat
     9541 tgcaaaaata gatttgttta ttcaaagaat cactcataat ttatggactg cacgtgaaaa
     9601 aaatgtgacg ttaattaagc aaataaatga tctcaaaatg tgtgtcaatg agtatattgg
     9661 agattgtact gatgagggat taaacgatcg tgaatttatt gcatcagttg ttgaccgggc
     9721 tatttttcat ttcgcgatta atagtatatg taatcccgag gataataaag atgcgacgcc
     9781 catagaacga tgtacttttg acgtagaaac taagaatggc cttccctcca cggttcagct
     9841 tttttatgag gaatctaaag ataatgaacc tttagcgaat atacatttgc aagcaatagg
     9901 ttctggtttt ttaacgtttg ttaatgcatg tcaggaatat gatgacaaca gcttaaaatt
     9961 attcgcttcg ctgttaattt cactttcata ttctagtgcc tacgtagatt tagcaggagc
    10021 agaaagagtg aagattaatg aatataatga gaactatctg acagctcagt ttgaagaatt
    10081 atctcaacgt gatatgaaga agtacctggg agagatgaag cgtctggcag atgggggaga
    10141 aatgaatttt gatgactatc tggataaaat gtcacttctg gtgaatgaag gaaagctcga
    10201 acctgatatt ttaagcaaaa tgcgagatgc tgcaccgaaa ttaattgact ttgctaagtc
    10261 gtttgaccca aactcaaagg aaaagattaa aatacttaca gacacttcta aattaattta
    10321 tgatttattc ggggttaaat cggagaaata atatgtgaag ttcttcgata gtatggaagg
    10381 cattatataa aaggacccaa tgcttattgg gttctttttt tctctatcag tgctattagc
    10441 agggagagat atcaccgtag tttaatgtgt gatttttatt tatcgtcgaa cctggattgt
    10501 ttatcattgg ccttaacaaa gctaacggct aataagatta tttccatcac ttcgtgagag
    10561 cttcatgcct tgagaggatc tcaattttct tttgcaatga gacaggcgct tcctgttgtt
    10621 atggtatagt accccgctat tgagcctcct gaacagtgat gctgaataac ataaccccat
    10681 gatatatcga taaaataatc tctacattta aaaatgcacg gtaattctga aatgcaaaaa
    10741 atcaaccaaa ccagcgcaat gcctgaaaaa actgacgttc actggagtgg tcggtttagc
    10801 gttgcaccaa tgctcgactg gacggacaga cattgccgct atttcttgcg tctgctttcc
    10861 cgcaatacgt tgctgtatac cgaaatggtg actacagggg cgattattca cggtaaaggt
    10921 gattacctgg cgtacagtga agaagaacat ccggtagcat tgcaactggg cggtagcgat
    10981 ccggcggcgc tggcgcagtg tgcaaagctg gcagaagcgc gcggatatga tgagatcaac
    11041 ctgaatgtcg gctgcccgtc tgaccgggtg cagaacggca tgtttggggc gtgtctgatg
    11101 ggtaatgcgc agctggttgc cgactgcgtg aaagcgatgc gcgatgtggt gtcgattccg
    11161 gtgacggtga aaacgcgtat tggcatcgac gaccaggaca gctatgaatt tctctgcgat
    11221 ttcatcaaca ccgtttccgg caaaggcgag tgtgagatgt tcatcatcca cgcacgtaaa
    11281 gcctggcttt cggggttaag cccgaaagaa aaccgtgaaa tcccgccgct cgattatccg
    11341 cgagtgtatc aattgaagcg tgactttccg catctgacaa tgtcgattaa cggtggtatc
    11401 aagtcgctgg aagaggctaa agcgcatttg caacatatgg atggcgtgat ggtcgggcgc
    11461 gaagcgtatc agaatccggg tattctggcg gcggtagacc gagagatctt tggttcctcg
    11521 gataccgatg ccgatccggt ggcggtagtg cgcgccatgt atccgtacat tgagcgtgaa
    11581 ctcagccagg ggacgtatct tggccatatt acccggcata tgctgggctt gttccagggt
    11641 attcctggcg cgcggcagtg gcggcgttat ttaagtgaaa atgcccataa agcgggtgca
    11701 gacattaatg tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc
    11761 aaaaaatagt caaattcacc acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc
    11821 aatagattat ttttggcatg attcttgtaa tgccagcaag agatttcata tttgggagag
    11881 catcatgctg gaactacttt ttgtgattgg cttttttgtc atgttgatgg tcaccggcgt
    11941 ttcgttgctg ggcattatcg ccgcgctggt tgtggcgacg gccattatgt tcctcggcgg
    12001 tatgctggca ttgatgatta agttgctgcc gtggttactc ctggcgattg cggtggtgtg
    12061 ggttattaag gcgattaaag caccaaaagt gccgaaatat cagcgttatg accgctggcg
    12121 ttactaaggg attgtgcgga tgatcacaac ctaaggtttt atccttagaa caaaatagga
    12181 attgataatc aaatctgtca ctattgcgcc tctaacagat tcatcgtgct gtaccctaca
    12241 tacagccgaa ctataaaaag aaagggcttc ccaggtggaa gccctatttc ttttatggaa
    12301 tcaacaggct ggaaccttgc gtcgcccggc tttccagaat ctcatgcgca cgctgcgcat
    12361 ccttcagcgg atatttctgc tgctcggcga catcgacctt aatcacaccg ctggcaatca
    12421 aagagaacag ttcattactg gcctcggtta attcctcccg cgtggtgata tagccttgca
    12481 gggaagggcg tgtcacatac aacgagcctt tctgattgag aatgcctaag ttcacgccgg
    12541 taaccgcacc tgatgagttg ccaaaactca ccattaagcc gcggcgttgc aggcaatcca
    12601 gcgaccgttc ccaggtgtct ctgcccacgg aatcgtacac cacgcgcact ttcttgccac
    12661 cggtgatctc ttttaaccgc tcgaccagat tctcttcgcg atagttaata acctgccatg
    12721 cgcccgcttt tagcgcactc tgcgcttttt gcgcggttcc cacagtgccg ataagtttcg
    12781 ccccaagggc ttttgcccac tggcaggcaa ttaagccaac gccgccagcc gctgcgtgga
    12841 acaggaactg ctcatcgggt ttaatttcat aggttttgcg cagcagataa taaaccgtta
    12901 agcctttcag gaaggatgcc gcagcttgct caaaagaaat tgccgcaggc agaatcgccg
    12961 ctttatccgc attaatgtta tgcaccgagc tgtaagcgcc taacgccgac tgcgcataga
    13021 ctacacgatc gcctgcctta atatgcttta caccactgcc gactttactc acgatgcctg
    13081 ccgcttcggt acctaatccg ctgggtagtg atggcggcgg gtaaaggccg ctgcggatat
    13141 acgtgtcgat aaaattgatg ccgatggctt tattttcgac ctggatttca ttctccgccg
    13201 gatcggcagg agtgaactct acggcttgaa gtacttccgg gccaccgtgc ttgtgaaatt
    13261 caattcgtgt tgccatgtgt cctccagaac gtatcgtcag ggtctgcttc atatggtaaa
    13321 gtttcgaccc attctttatc tcggtaactc cattcactat ggcaggaaat aaacccttca
    13381 acaaacagca ggctgaaccc cgcgaacgcg atccacaagt tgccgggctg aaagtgcctc
    13441 cgcactcgat cgaagcggag cagtcggtgt tgggcggttt aatgctggat aacgaacgct
    13501 gggacgatgt agccgagcgt gtggtggcag acgattttta cacccgccca caccgtcata
    13561 tctttactga aatggcgcgt ttacaggaaa gcggtagtcc tatcgatctg attacccttg
    13621 cggaatcgct ggaacgccag gggcaactcg atagcgtcgg tggtttcgct tatctggcgg
    13681 agctgtcaaa aaatacgcca agtgcggcga acatcagtgc ttatgctgac atcgtgcgtg
    13741 aacgtgccgt tgttcgcgag atgatttcgg ttgcgaatga gattgccgaa gccggttttg
    13801 atccgcaggg gcgcaccagc gaagatctgc tggacctggc tgaatcccgc gtctttaaaa
    13861 ttgccgaaag tcgtgcaaac aaagacgaag ggccgaagaa catcgctgat gtgctcgacg
    13921 ccacggtggc gcgtattgag cagttgtttc agcagccaca cgatggcgtt accggggtaa
    13981 acaccggtta tgacgatctc aacaaaaaaa ccgctggctt gcagccgtcg gatttgatca
    14041 tcgtcgccgc gcgtccgtcg atgggtaaaa ccacatttgc gatgaacctc gtcgaaaacg
    14101 cggcgatgtt gcaggataaa cctgtactta tcttctcgct ggagatgcca tcagaacaga
    14161 ttatgatgcg ttctctggcg tcgctgtcgc gcgttgacca gactaaaatc cgtaccgggc
    14221 agctcgatga tgaagactgg gcacgtattt ccggcaccat gggtattttg ctcgaaaaac
    14281 gtaatatcta tatcgatgat tcctccggcc tgacgccaac ggaagtgcgt tcccgcgcgc
    14341 gccgtattgc ccgtgaacac ggcggcatcg ggcttatcat gatcgactac ctgcaactga
    14401 tgcgcgtacc ggcgctttcc gataaccgta cgctggaaat tgcagaaatc tcccgctcgc
    14461 tgaaagcact ggcgaaagaa ctgaacgtgc cggtggtggc gctgtcccag ttgaaccgtt
    14521 ctctggaaca acgtgccgac aaacgtccgg tcaactccga cctgcgtgaa tctggctcta
    14581 tcgagcagga tgcggacttg atcatgttta tctatcgtga tgaggtgtat cacgaaaaca
    14641 gtgatttaaa aggcatcgcg gaaattatta tcggtaaaca acgtaacggc ccaatcggga
    14701 cggtacgcct gacctttaac ggtcaatggt cgcgcttcga caactatgcg gggccgcagt
    14761 acgacgacga ataataatta ttttatgaat taggtaatta aagcaaacac ttatcaagga
    14821 acacaaatgc aagcggcaac tgttgtgatt aaccgccgcg ctctgcgaca caacctgcaa
    14881 cgtcttcgtg aactggctcc tgccagtaaa atggttgcgg tggtgaaagc gaacgcttat
    14941 ggtcacggtc ttcttgagac cgcgcgaacg ctccccgatg ctgacgcctt tggcgtagcc
    15001 cgtctcgaag aagctctgcg actgcgtgcg gggggaatca ccaaacctgt actgttactc
    15061 gaaggctttt ttgatgccag agatctaccg acgatttctg cgcaacattt tcataccgcc
    15121 gtgcataacg aagaacagct ggctgcgctg gaagaggcta gcctggacga gccggtaacc
    15181 gtctggatga aactcgatac cggtatgcac cgtctgggcg taaggccgga acaggctgag
    15241 gcgttttatc atcgcctgac ccagtgcaaa aacgttcgtc agccggtgaa tatcgtcagc
    15301 cattttgcgc gcgcggatga accaaaatgc ggcgcaaccg agaaacaact cgctatcttt
    15361 aatacctttt gcgaaggcaa accaggtcta cgttccattg ccgcatcggg tggcattctg
    15421 ctgtggccac agtcgcattt tgactgggtg cgcccgggca tcattcttta tggcgtctct
    15481 ccgctggaag atcgctccac cggtgccgat tttggctgtc agccagtgat gtcactaacc
    15541 tccagcctga ttgccgtgcg tgagcataaa gccggagagc ctgtcggtta tggtggaacc
    15601 tgggtaagcg aacgtgatac ccgccttggc gtagtcgcga tgggctatgg cgatggttat
    15661 ccgcgcgccg cgccgtccgg tacgccagtg ctggtgaacg gtcgcgaagt gccgattgtc
    15721 gggcgcgtgg cgatggatat gatctgcgtc gacttaggtc cacaggcgca ggataaagcc
    15781 ggggacccgg tcattttatg gggcgaaggt ttgcccgtag aacgtatcgc tgaaatgacg
    15841 aaagtgagcg cttacgaact tatcacgcgc ctgacttcaa gggtcgcgat gaaatacgtg
    15901 gattaatcgt tctgtaatat ttgattgttt gtgccggatg cggcgtgaat gccttatccg
    15961 gccaataaaa tcctaccaat tcaataagtt gatattcttt catgctctta tgaaggccgt
    16021 gcctctggcg gatgtacgtt tgtcatgagt ctcactctgt tgctaattgc cgttcgcccc
    16081 tgaacatcca ctcgatcttc gccttcttcc ggtttattgt gttttaacca cctgcccgta
    16141 aacctggaga accatcgcgt gtttcaaaaa gttgacgcct acgctggcga cccgattctt
    16201 acgcttatgg agcgtttcaa agaagaccct cgcagcgaca aagtgaattt aagtatcggt
    16261 ctgtactaca acgaagacgg aattattccc caactgaaag ccgtggcgga tgcggaagcg
    16321 cgcctgaatg cgcagcctca tggcgcttcg ctttatttac cgatggaagg gcttaatagc
    16381 tatcgccatg ccattgcgcc gctgctgttt ggtgcggacc atccggtact gcaacaacag
    16441 cgcgtagcaa ccattcaaac ccttggcggc tcaggggcat tgaaagtggg cgcagatttc
    16501 ctgaaacgct acttcccgga atcaggcgtc tgggtcagcg atcctacctg ggaaaaccac
    16561 gtagcaatat tcgccggggc tggattcgaa gtgagtactt acccctggta tgacgaagcg
    16621 actaacggcg tgcgctttaa tgacctgttg gcgacgctga aaacattacc tgcccgcagt
    16681 attgtgttgc tgcatccatg ttgccacaac ccaacgggtg ccgatctcac taatgatcag
    16741 tgggatgcgg tgattgaaat tctcaaagcc cgcgagctta ttccattcct cgatattgcc
    16801 tatcaaggat ttggtgccgg tatggaagag gatgcctacg ccattcgtgc cattgccagc
    16861 gctggattac ccgctctggt gagcaattcg ttctcgaaaa ttttctccct ttacggcgag
    16921 cgcgttggcg gactttctgt tctgtgtgaa gatgccgaag ccgctggccg cgtactgggg
    16981 caattgaaag caacagttcg ccgcaactac tccagcccgc cgaattttgg tgcgcaggtg
    17041 gtggctgcgg tgctgaatga cgaggcattg aaagccagct ggctggcgga agtagaagag
    17101 atgcgtactc gcattctggc gatgcgtcag gaactggtga aggtattgag cacagagata
    17161 ccagaacgca atttcgatta tctgcttaat cagcgcggca tgttcagtta taccggttta
    17221 agtgccgctc aggttgaccg actacgtgag gagtttggta tttatctcat cgccagcggt
    17281 cgcatgtgtg tcgccgggtt aaatacggca aatgtacaac gtgtagcaaa ggcgtttgct
    17341 gcggtaatgt aatgcaggaa agcaggctgg agttatccag cctgcagtga aattaaactg
    17401 tcgtcgcttt cactcttgct ttatagatga tttttttgat gccatcgttc tacgtgagag
    17461 gtaataaaac gttgttagtt ctaacttgtt aagtttatcc caatgttctg gagtgctttt
    17521 ccctgtttta ccgtgtggag taataatttc tgcacatcta accattatta aatttaataa
    17581 taagtatcgg gtttgttacg atagaatatt tctatatcct caagaatatg ctttgcctta
    17641 ttgcctgtga ctttctgaga ttcaattata gtatcgttaa attctaatgt taaagagaac
    17701 tctttttttc cgctttgtgt aagtttaacc ttatattgat taccaatatc aaataaaaag
    17761 gttctgtaat gagaataatc accttt
//
LOCUS       DAOTRK010000100        17603 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.100, whole genome shotgun sequence.
ACCESSION   DAOTRK010000100 DAOTRK010000000
VERSION     DAOTRK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.100"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            58..1197
                     /gene="arnB"
                     /locus_tag="QIE41_003180"
     CDS             58..1197
                     /gene="arnB"
                     /locus_tag="QIE41_003180"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDS3532035.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARLLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIALQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVNEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1201..2169
                     /gene="arnC"
                     /locus_tag="QIE41_003181"
     CDS             1201..2169
                     /gene="arnC"
                     /locus_tag="QIE41_003181"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDS3532036.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2169..4151
                     /gene="arnA"
                     /locus_tag="QIE41_003182"
     CDS             2169..4151
                     /gene="arnA"
                     /locus_tag="QIE41_003182"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDS3532037.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPEDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHSHAPAAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMHETIDETLDFFLRTVDLTDKPS"
     gene            4148..5038
                     /locus_tag="QIE41_003183"
     CDS             4148..5038
                     /locus_tag="QIE41_003183"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDS3532038.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTLVDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGMPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5038..6690
                     /gene="arnT"
                     /locus_tag="QIE41_003184"
     CDS             5038..6690
                     /gene="arnT"
                     /locus_tag="QIE41_003184"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDS3532039.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVASQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6687..7022
                     /gene="arnE"
                     /locus_tag="QIE41_003185"
     CDS             6687..7022
                     /gene="arnE"
                     /locus_tag="QIE41_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDS3532040.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7022..7408
                     /gene="arnF"
                     /locus_tag="QIE41_003186"
     CDS             7022..7408
                     /gene="arnF"
                     /locus_tag="QIE41_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDS3532041.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7402..7668)
                     /gene="pmrD"
                     /locus_tag="QIE41_003187"
     CDS             complement(7402..7668)
                     /gene="pmrD"
                     /locus_tag="QIE41_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDS3532042.1"
                     /translation="MEWLVKKSCCDKQDNRHVLKLCDAGGTIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7778..9133)
                     /gene="menE"
                     /locus_tag="QIE41_003188"
     CDS             complement(7778..9133)
                     /gene="menE"
                     /locus_tag="QIE41_003188"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDS3532043.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQDVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQLVALVNDEGWYAT
                     RDRGELHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9130..10092)
                     /gene="menC"
                     /locus_tag="QIE41_003189"
     CDS             complement(9130..10092)
                     /gene="menC"
                     /locus_tag="QIE41_003189"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDS3532044.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDALPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPRKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVEVDALERLL"
     gene            complement(10092..10949)
                     /gene="menB"
                     /locus_tag="QIE41_003190"
     CDS             complement(10092..10949)
                     /gene="menB"
                     /locus_tag="QIE41_003190"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDS3532045.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10964..11722)
                     /gene="menH"
                     /locus_tag="QIE41_003191"
     CDS             complement(10964..11722)
                     /gene="menH"
                     /locus_tag="QIE41_003191"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDS3532046.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFANYSRLY
                     VDLPGHGGSAAIRVDGFDDVTGLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVIVEGGHPGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDEQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11719..13389)
                     /gene="menD"
                     /locus_tag="QIE41_003192"
     CDS             complement(11719..13389)
                     /gene="menD"
                     /locus_tag="QIE41_003192"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDS3532047.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWHDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTNGAQTLQQLLAQVSHL"
     gene            complement(13478..14773)
                     /gene="menF"
                     /locus_tag="QIE41_003193"
     CDS             complement(13478..14773)
                     /gene="menF"
                     /locus_tag="QIE41_003193"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDS3532048.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPKHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQRLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14852..15157)
                     /gene="elaB"
                     /locus_tag="QIE41_003194"
     CDS             complement(14852..15157)
                     /gene="elaB"
                     /locus_tag="QIE41_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDS3532049.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15212..15673)
                     /locus_tag="QIE41_003195"
     CDS             complement(15212..15673)
                     /locus_tag="QIE41_003195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS3532050.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMNKTLET
                     CTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15738..16655
                     /gene="rbn"
                     /locus_tag="QIE41_003196"
     CDS             15738..16655
                     /gene="rbn"
                     /locus_tag="QIE41_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDS3532051.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIVQPLTIYGPQGIREFVE
                     TTLRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16846..>17603
                     /locus_tag="QIE41_003197"
     CDS             16846..>17603
                     /locus_tag="QIE41_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDS3532052.1"
                     /translation="MVTVVSNYCQLSQTQLCQTFAEKFTVTEELLQSLKKTALSGDEE
                     SIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATKH
                     PLKSHDSDDLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
                     KNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPLWPK
                     AVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCI"
BASE COUNT         4162 a         4736 c         4428 g         4277 t
ORIGIN      
        1 caacactctt tatagcaaat ataagtggac aggtattcaa tggcggaagg aaaagcaatg
       61 tcagaatttt tgcctttttc gcgaccagca atgggcgtgg aggaactcgc tgcagttaaa
      121 gaggttctcg aatccggttg gatcacaacc ggtccgaaga atcaggcgct tgaacaagcc
      181 ttttgccagt tgacgggaaa tcagcatgcc atcgcggtca gttcagccac cgccggaatg
      241 catatcacgc taatggcgtt ggaaattggc aagggcgatg aagtgattac gccttccctg
      301 acctgggttt caaccctcaa tatgatttcc ttgttgggtg caacgccggt aatggtggat
      361 gtcgaccgcg atacgctgat ggtcacgcct gaagcgatcg agtcagccat tacgccacgc
      421 actaaagcca tcattccggt gcattatgcc ggtgcgccag cagatattga cgccattcgc
      481 gccattggcg aacgttacgg catcgcagtt atcgaagatg ctgcccatgc cgtcggtacg
      541 tattacaaag ggcgacatat tggcgcaaaa ggtaccgcta ttttttcgtt tcacgccatc
      601 aaaaatatta cctgtgcgga aggtggcctg attgttacag ataatgaaaa ccttgcccgc
      661 ctgctacgga tgctgaaatt tcacggtctg ggtgtcgatg cctatgacag acaaacctgg
      721 ggccgtgcac cgcaagctga agtcttaacg ccgggctata agtataatct gaccgatatt
      781 aacgccgcga ttgccctgac ccagttagcc aaattagagc acctcaacac ccgtcggcgc
      841 gaaattgctc tgcaatatca gcaagcactg gcagctctcc cctttcagcc attaagcctt
      901 cccgcctggc cgcacgttca cgcctggcat ctgtttatta ttcgtgtcaa tgaacaacgt
      961 tgtggtatca gtcgcgatgc gttgatggaa gcgttaaaag aaagaggcat tggtaccggg
     1021 ttacatttcc gcgccgctca cacacaaaaa tattatcgcg agcgttttcc cacgctgtcg
     1081 ttaccgaata ccgaatggaa tagcgaacgc atctgttcgt tgccgctgtt cccggatatg
     1141 accaccgccg atgccgaccg cgtcatcaca gcccttcagc aactcgcagg acaataagcc
     1201 atgtttgaaa tccaccctgt taagaaagtc tcggtggtta ttcccgttta taacgagcag
     1261 gaaagcttac cggaattaat caggcgcacc acaaaagcct gtgaatcact ggggaaagag
     1321 tatgagatcc tgctgattga tgacggcagt agcgataatt ccgcgcatat gctggtcgaa
     1381 gcctcacaag cggagaacag ccatattgtg tctattttgc ttaaccgcaa ttacgggcaa
     1441 cattcagcga ttatggcggg attcagtcac gttactggcg acttaattat tacccttgat
     1501 gccgatctcc agaatccgcc agaggagatc ccccgcctgg tggcaaaagc cgatgaaggc
     1561 tacgacgtgg taggaactgt gcgtcagaac cgccaggaca gctggtttcg taaaacggca
     1621 tcgaagatga ttaaccggct tattcagcgc accaccggca aagcgatggg tgactacggt
     1681 tgtatgctgc gcgcctatcg ccgtcatatt gtcgatgcga tgttgcactg ccatgaacgc
     1741 agcaccttta tcccgattct ggcgaatatc ttcgcccgcc gtgccattga aattccagtg
     1801 caccatgccg agcgtgagtt tggtgaatcc aaatacagtt ttatgcgcct gattaatttg
     1861 atgtacgacc tggtgacctg ccttaccaca acgccgctac gtatgctgag tctgcttggc
     1921 agcattattg cgattggagg ttttagcatt gcggtgctgc tggtgatttt acgcctgacc
     1981 ttcggaccac aatgggcggc agaaggcgtc tttatgctat ttgccgtgct gtttactttt
     2041 attggcgctc agtttatcgg catgggattg ctcggtgaat atatcggcag gatctacacc
     2101 gatgtccgcg cccgcccccg ctattttgtt cagcaagtta tccgtccatc cagcaaggaa
     2161 aatgaataat gaaaaccgtc gtttttgcct accacgatat gggatgcctc ggtattgaag
     2221 ccctgctgtc tgccggttac gaaattagcg ccatttttac ccataccgat aatcccggtg
     2281 aaaaagcctt ttatggttcg gtggctcgtc tggcggcgga aagaggcatt ccggtttatg
     2341 cgccggataa cgttaatcat ccgctgtggg tggaacgtat tgcccaactc tcaccagagg
     2401 tgattttctc tttttattat cgccatctta tttgcgacga gattttgcag ctcgctcccg
     2461 caggtgcatt taatctgcat ggttcgctgt taccaaaata tcgtggtcgc gcgccgctga
     2521 actgggtgct ggtgaacggt gaaacggaaa ctggcgttac attgcaccga atggtgaaac
     2581 gtgccgatgc cggggccatt gtggcgcaac tgcgcattgc cattgcgcca gacgatatcg
     2641 ctattacgct gcatcataaa ttgtgccatg ccgcgcgcca gctactggaa cagacattac
     2701 ccgccattaa acacggtaat attctggaaa tcgcccagcg cgaaaacgaa gccacctgtt
     2761 ttggtcgcag aacaccggaa gacagtttcc tcgaatggca taaaccggca tccgtgctgc
     2821 acaacatggt gcgtgccgtt gccgatccgt ggccgggtgc cttcagctat gttggtaatc
     2881 agaaatttac cgtctggtcg tcacgcgtgc attctcatgc gcccgcagca caaccgggga
     2941 gcgtgatttc tgttgcgcca ctgctgattg cctgtggcga tggcgcgctg gaaatcgtca
     3001 ccggacaggc gggcgacggc attactatgc agggctcgca attagcgcag acgctgggcc
     3061 tggtgcaagg ttcacgcttg aatagccagc ctgcctgtgc cgcccgacgc cgtacccggg
     3121 tactcatcct cggggtgaat ggctttattg gcaaccatct gacagaacgc ctgctgcgcg
     3181 aagatcatta tgaagtttac ggtctggata tcggcagcga tgcgataagc cgttttctga
     3241 atcatccgca ttttcacttt gtcgaaggcg atatcagtat tcattccgaa tggattgagt
     3301 atcacgtcaa aaaatgtgat gtcgtcttgc cgctggtggc gatagccacg ccgattgaat
     3361 atacccgcaa cccactgcgc gtatttgaac tcgattttga agagaatctg cgcattatcc
     3421 gctactgcgt gaagtaccgt aagcgaatca tcttcccgtc gacttcagaa gtttatggga
     3481 tgtgtagcga taaatacttc gatgaggatc attctaattt aatcgtcggc ccggtgaata
     3541 aaccacgctg gatttattcg gtgtctaaac aattacttga tcgggttatc tgggcctatg
     3601 gcgaaaaaga gggtttacag tttaccctct tccgcccgtt taactggatg ggaccacgac
     3661 tggataacct taatgcggca cgaatcggca gctcccgcgc tattacgcaa ctcattctca
     3721 atctggtaga aggttcacca attaagctaa ttgatggcgg aaaacaaaaa cgctgcttta
     3781 ctgatatccg ggatggtatc gaggcgttgt accgcattat cgaaaatgcg ggaaatcgct
     3841 gcgacggcga aattatcaac attggcaatc ctgagaacga agcgagcatt gaggaactgg
     3901 gcgagatgct gctggcgagc ttcgaaaaac atccgctgcg ccatcacttc ccaccgtttg
     3961 cgggctttcg tgttgtcgaa agtagcagct actacggcaa aggatatcag gacgtagagc
     4021 atcgtaaacc gagcatccgc aatgcccgcc gctgccttga ctgggaaccc aaaattgata
     4081 tgcatgaaac catcgacgaa acgctagatt tcttcctgcg caccgttgat cttacggata
     4141 aaccatcatg accaaagtcg gcttacgcat tgatgtcgat acctttcgtg gcacccgtga
     4201 aggcgtgccg cgtctgctgg aaatcttgag taagcataat attcaggcca gcattttttt
     4261 cagcgtcggc ccggacaata tgggccgcca tctctggcga ctggtgaagc cacagttttt
     4321 gtggaagatg ctgcgctcaa acgcggcatc gctttatggc tgggatattt tactggcagg
     4381 tacggcctgg ccgggtaaag agattggtca tgccaatgcc gatatcattc gtgaagcagc
     4441 taaacatcac gaagtcggcc tgcacgcctg ggatcaccat gcctggcaag cccatagcgg
     4501 taactgggat cggcaaacat tggtcgacga tattgcacgc ggccttcgca ccctggaaga
     4561 gatcataggt caaccggtaa cctgttctgc cgctgcgggc tggcgtgccg accagcaggt
     4621 gatcgaagca aaagaagcgt tccatttgcg ctacaacagc gattgtcgtg gggccatgcc
     4681 gtttcggcca ctgctcgaat caggaaaccc tggcactgcg caaattccgg tgaccttacc
     4741 cacctgggat gaagtgattg gtcgggatgt gaaagcagaa gattttaacg gttggttact
     4801 caaccgcatc ctgcgagata aaggcatgcc ggtttatacc attcatgcag aagttgaagg
     4861 ctgcgcttat cagcataatt ttgtggatct cctgaaacgg gcagcccagg aaggcgtgac
     4921 attttgccct ttaagcgaac tgttatcagg gacgttgccg ctcggacaag ttgttcgcgg
     4981 aaatattgcc ggacgtgaag gctggctggg ttgccaacaa attgcgggta gtcgttgatg
     5041 aaatcggtac gttaccttat cggcctcttc gcgtttattg cctgctatta cctgttaccg
     5101 atcagcacgc gtctgctatg gcaacccgat gaaacgcgtt atgcggaaat cagtcgggaa
     5161 atgctggcat ccggcgactg gattgtgccc catctgttag ggctacgtta tttcgaaaaa
     5221 cccattgccg gatactggat taacagcatt gggcaatggc tatttggcgc gaataacttt
     5281 ggtgtgcggg caggcgttat ctttgcgacc ctgttaactg ccgcgctggt gacctggttt
     5341 actctgcgct tatggcgcga taaacgtctg gctctactcg ccgcagtgat ttatctctca
     5401 ttgtttattg tctatgccat tggcacttat gccgtgctcg atccgtttat tgcattctgg
     5461 ctggtagcgg gaatgtgcag cttctggctg gcaatgcagg cacagacgtg gaaaggcaaa
     5521 agcgcgggat ttttactgct gggaatcact tgcggcatgg gggtgatgac caaaggtttt
     5581 ctcgcccttg ccgtgccggt attaagcgtg cttccatggg ttgcatcgca aaaacgctgg
     5641 aaagatctct ttatttacgg ctggctggcg gttatcagtt gcgtactgac ggttctcccc
     5701 tggggactgg cgatagcgca gcgggagcct gacttctggc actatttttt ctgggttgag
     5761 catattcaac gctttgcaat ggatgatgct cagcatagag ctccgttctg gtactacttg
     5821 ccggtcatca ttgccggtag cctgccgtgg ctgggattac tccccggagc actgtacgca
     5881 ggctggaaaa accgcaagca ttccgcaacc gtctatttgt tgagctggac gataatgccg
     5941 ctgctgtttt tctccgtcgc taaaggcaaa ttgcccacct atattctttc ctgctttgca
     6001 cctctggcaa tgctgatggc gcattacgct ttgctggcag caaaaaataa tcctctggcg
     6061 ctgcggatta atggctggat taacatcgct tttggtgtca ctggcattat tgccacattt
     6121 gtggtctccc catggggacc aatgaacacg ccagtgtggc aaaccttcga gagctataaa
     6181 gtcttttgtg cctggtcgat tttttcgcta tgggcatttt tcggctggta caccttaaca
     6241 aacgtcgaaa agacctggtc ttttgccgcg ctttgcccgc tggggctggc gttgctggta
     6301 ggattttcaa ttcctgacag agtcatggaa ggaaaacatc cgcaattttt tgtcgagatg
     6361 acacaagaat cacttcagcc aagccgctat attcttactg atagcgtcgg tgttgccgca
     6421 ggtctggcat ggagcctgca acgcgatgac atcatcatgt atcgccagac aggtgagttg
     6481 aaatacggcc ttaattatcc ggatgcgaaa gggagatttg tcagcggtga tgagttcgca
     6541 aactggctta atcaacatcg tcaggagggg attattacac tcgtgctttc ggttgaccgc
     6601 aatgaagata tcaacagtct cgccattccg cccgcagatg ccatcgatcg tcaggagcgt
     6661 ctggtgctga ttcagtatcg tcccaaatga tctggctaac attagtcttt gccagcttac
     6721 ttagcgttgc cgggcagttg tgtcagaaac aggcaacctg ctttgtggcg ataaacaaac
     6781 ggcgcaaaca tatcgtgctg tggctgggac tggcgctggc ttgtcttggt cttgccatgg
     6841 tgctctggct gctggtcttg cagaacgtac cggtaggcat tgcctacccg atgttaagtc
     6901 tgaattttgt ctgggtgacg ctggctgcag taaaactgtg gcacgaaccg gtatcgccgc
     6961 gtcactggtg tggggtggcg ttcattattg gtggcattgt gatcctcggg agtaccgtgt
     7021 aatgggcctg atgtggggat tattcagcgt cattatcgcc tccgctgcgc aattaagcct
     7081 gggttttgcg gcgagtcatc tgccgccgat gacgcactta tgggatttta tcgcggcgct
     7141 gttagctttt ggcctggatg cccggatact gttactcggg ctgctgggtt atctgctttc
     7201 cgttttttgt tggtataaaa cactacataa acttgcacta agcaaagcct atgccttgct
     7261 cagtatgagt tatgtgctgg tatggattgc ttcaatggta cttcccggct gggaagggac
     7321 tttttcgctg aaagcactac tgggagtagc ctgtattatg agcgggttga tgctgatttt
     7381 cctgcccacg acaaaacaac gttactgagt tttccctgcc actttacact gccgttccca
     7441 ctcatcgggg ctataactgc ttgccgagag gactttcacc gtatgcagct tttcacgatt
     7501 aatacaataa agcgcattct gcaaaggcga gagtaaatcc cccactttca cggcgaagtc
     7561 actcttcact tcggcgatca tttttatcgt gccaccagca tcacaaagct taagtacatg
     7621 tctgttatct tgtttatcac agcatgactt tttaaccagc cattccattg caccaccctg
     7681 cttgataaga attttcacat taatttacag attaacagat aaacctatta tcaacggcaa
     7741 tggaatggca aaattgcgaa gtgaaactaa tgatttctta atcctggcgt tgcacccact
     7801 cttttagcgc ctgacgtgaa atcttaatgc cgccgttttt cagttcaggc ggcagcgtta
     7861 accaacgcac cggttgctga aaacgggcca gcttatcttt cacccattca ctaagatcaa
     7921 cggtttgctg gtcatactcc accaccgcta ccggccgatg accaaactcc ttgtcggcaa
     7981 cggggacgat aaacacctgc aaaaccgcag gatgtgcagc aataacgcgc tcgacttctt
     8041 ccggctgaat accctcaccg ccactgaaga acagattgtc taaacgcccg acaatggtca
     8101 gcttgccatt atgcagctca ccgcggtcgc gcgtagcgta ccagccttcg tcattaacca
     8161 gtgccaccag ttgcccgtta cgccagtaac cttcagccat actggcagcc cgcagccaca
     8221 cttcatcatt aacgattttc acttcccgac ccggcagcgg cgaaccaacg tctgccagac
     8281 cgtcggcttc tttcgcacac accgtggagg caaactcggt cagaccatag ccgcaaaagc
     8341 aacgaatccc ctgctcgcgc gcctgttccg tcaactcgac cgggatagcc gcgccgccaa
     8401 gtagcaccgc tttcagggaa acggaactac ggttaaccag caaacgccag agttgtgttg
     8461 gcaccagtga agcgtgagta cagcctgcca gcatttgctc caatggctgt ttatctcgta
     8521 ccgtcatccg cgcaccagcg tataaccagc gccacataat tccctgaccg gagacgtgaa
     8581 acagcggtaa agagagcaac cagtcatcgt gctcgccaaa cggtatcagc gataacacac
     8641 cttgcgcact ggcaagatgg gcctgataag tatgaacagc ggctttcggc aaaccggtag
     8701 agccggaggt caacgtcatt gagcacagac gcgtcggctg ccacgcagcg gcatgtgcgc
     8761 cttcaaccag ctgaatgtgc agcgacgtta acgccggaaa cgtgttttcc ccatccggca
     8821 ccagcgcaaa ttgcagcgtc agattgggca gcaattcttc aagcaacggt tgcggcagct
     8881 gagggttcac gggcagcacc cgcgccccgc attgcagtaa cgccagccag gcgagcagcg
     8941 tttgcggcgt attccacgcc cgcaacatca cgccgctgcc ctcaacaacg tcctgcgccg
     9001 caaagccgga ggctaattca tcgacgcgag cacaaagctc gcgccagtta agttgctcgt
     9061 cattaagacg taaggcgatg gcttctcccc gcacttgccg ccagtgacgc cacggccagt
     9121 cagagaagat cataacagcc gctccagtgc atcaacttcc actaacggta gcgggctacc
     9181 cggccagcga cgaacctgct gcgcctgcat cagatccagc gtgtccagcc ccggaatggt
     9241 gtctggcgtt aaccaggcgg caatccgcgc cagttgcgtt aagcctaagc tcgactcaat
     9301 agaagaactg atcaccgccg tcagccccaa cgcgtgcgcc gcctgcacct gctcgcgtac
     9361 tttatccagg ctgcccgtga gcgtgggctt gataaccacc gcgcgcacgc cctcttcagc
     9421 tacaaaggca aaatctggct cgcgcaggct ttcatcccag gcaatggcaa tgccggtttc
     9481 acgggcaaac gctcgcgaat catcgcgggt tttgcacggc tcttcgagaa aagcaatgcg
     9541 gtggcgataa tccgggttaa cgtatttggc aaactgctgg cctttccgcg gcgtccaggc
     9601 gcggttcgcg tcaagacgca gatgcagatc cggaattgcc tccagcaaca gattgaccac
     9661 catgccgtcg cgcacggctt cgtacaatcc cactttgacc ttcgccactt tctcgccagg
     9721 catatcggca agtttgagga tcagatcgtc cggatcgcca ttacacagcg gtgccgcacg
     9781 gtagttggct gcttgcggca acgcatcggt cagttctgcc aatgcacagc ttacgccaaa
     9841 ggccacggaa ggcatctgcg gtagctcgca atcgcctgcc agccagttat ttacccaggc
     9901 aagcagcacg ctttgcgcct cttcccaggt ttcctggctg aagcccggca gtggggagat
     9961 ctccccccac ccttcgcgct cgccctcacg caggcaaacg tacagcccgt cgcgggtttt
    10021 taaccgcctg tcgcgcagaa ccacccccgc gtccatgggg atctgccagc ggtatacctg
    10081 cgcgctacgc attacggatt ccgtttgaat ttgctgaagt caggctgacg tttctggttg
    10141 aaagcgttgc gaccttcctg accttcttcc gtcatataga acagcatggt ggcgttacct
    10201 gccagctcct gcagacctgc ctgaccgtca cagtcggcgt tcagtgcagc tttcaggcag
    10261 cgcagcgcca tcgggctgtt ttgcagcatt tcgcggcacc aacggacggt ttctttttcc
    10321 agatccgcca gcggtaccac ggtgttcaca aggcccatat ccagcgcctg ttttgcgtcg
    10381 tactgacggc acaggaacca gatttcacgc gcttttttct gcccgacgat gcgagccatg
    10441 taggaagcgc cccagccgcc gtcgaaggaa ccgactttcg ggccagtctg accgaagatg
    10501 gcattatctg ccgcgatagt caggtcgcac atcatgtgca gaacgtgacc gccgccgatg
    10561 gagtagccag ccaccatcgc gacaaccggt ttcggacagg tacggatctg acgctggaag
    10621 tccagcacgt tcaggtgatg tacgccggaa tcatctttat agccgccgta atcaccacgc
    10681 actttctggt caccaccgga gcagaacgct ttatcgcctg caccagtcag aatgatcacg
    10741 ccgatgttgt cgtcataacg cgcatctgcc agcgcctgga tcatctcttt taccgtcaga
    10801 ggacggaagg cattgcgcac ctgcggacga ttaatggtga tttttgcgat accgtcggtg
    10861 gatttttcat aacgaatgtc ctcgaaacct tcggagcagt cgtgccattc aaccggtgcg
    10921 taaagcattg cttcatcagg ataaatcata ttgtgtcctt tagtcagaaa cgcaagatct
    10981 gcgccagact tgcgattacg ccagcgggat tttcccgatg cgcgttatgt ccggcgcgag
    11041 gaatgacatg gcattcggca gccagttccg ccgccagggc gcggaatttg ctgtcacgtt
    11101 caccacataa ataataaaac gcaaatgtgc gggcgctaag gttagcacgt aaatcgggct
    11161 ggacggcgag agaagtcgcc tccagcatgg cggcaagcgt tgcgccatta ttgttgctgc
    11221 gcagcgcaac cagctcccgg cgttgttcgt cattgagtga ggcaaaaaca ggctgttgat
    11281 accagtcagc aaataccgcc gttaacggtt ctgtgcgaaa acgctgcgcc cattggcgat
    11341 cggaacgctg acgttccgtg cgttgttcag cattttgcaa cccagggtgc ccgccttcga
    11401 caataacccc acaaagcccc ggcatcccct gacaagccgc catcatcgcc acccgtccgc
    11461 caagcgagta ccccaccagc cagaagttaa ggatgttgta actaaccaga gttttgcgca
    11521 gtaagccggt gacatcatca aatccatcga cgcgaatcgc cgccgaacca ccgtgacctg
    11581 ggagatcaac atacaaccgc gagtagttgg caaacacctc gcccacttct tgccattcat
    11641 ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt ttaccgtgtt
    11701 ttgcctgcgc gtgcaggatc ataaatggct aacctgcgcc agcagttgct ggagggtttg
    11761 cgcaccattg gtgtcgttaa ccaccatttc aattaccgtg gtggtcggcg tgcgccaggc
    11821 gtcggcaagt gccgtttcaa gttcctgcca gttttgcgga cgatgatatt tcagctcgaa
    11881 catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac gctcgcgctc
    11941 gtttttcggc gttggcaaca gcgagaaaat ttgcccgcca ttgttgttca ccacaattaa
    12001 taccagcggc gcggaaacct gacgtaataa cgccagcgcg ttgagatcgt aaagtgctga
    12061 gagatcgccc acaatcgcca gcgtcggttt gccactggcc cgctgaacgc cagcagcagt
    12121 tgagagcagc ccgtcgatac cgctggcacc tcggttgctg tacaccgggt aacctgccgg
    12181 aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccgacaa acaattgccc
    12241 ctgttcaggc aaatagtcgc tgatgcgatg cgccagttgc gcttcgccaa acgcatcgcg
    12301 acgggcaata accgcctgca ttgcctgttc agccaggcgc gggatttcaa cgcaccaggg
    12361 ctggcgtttt tctgccggat gttgctccag ccagtcggca atattggcaa ttaagcgacg
    12421 tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc agtactcttc
    12481 gggttcacag cttgcctgcc attgcaggag ccgtttgccc gtcaggctgc ttcccagttg
    12541 caccacaatt tgcgcctgtt gcagttcgct ggtcgctttg gcattgccta accagagatc
    12601 ggcacacggc agcggctgtc cggtttgcga cagcacgtca ccgatcagcg gccagccaag
    12661 agtttgcgcc cacagtgcga cttttttgcc ctcttccgca ctcatgcgcc cggcaaccac
    12721 cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt ccagacgagg
    12781 cgcttcacgc agccacggtt tgtcgtcatg ccaccagtcg cccagccgct gttgccagct
    12841 aagcccggtg tcgtccattt cgccatacag cggttcagca aacgggcagt tgatatggac
    12901 tccaccagca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc aacgtgcggg
    12961 gatatcctgg gtcgggcgcg gcagtgaaat actgtgcgtg gggtgagagg cgaacattcc
    13021 cggctgacga atcgcctgat tcgcgccgca gtcaattagc tccggcgggc gatcggcggt
    13081 taacaggatc agtttttctc cggttaaccc agcttcaatc agtgcaggat agagatttgc
    13141 taccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt tcgccagccc
    13201 cagcgccaga tgccccagtc cacgctcatc gaaatgggtg tggtgaatga atgcggaatt
    13261 ctccgccgcc gctaacgtta acggtgtaga acgcgagcca ggggcgatac agatgtgtct
    13321 tacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt taaatgcgct
    13381 tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc tacaaaaata
    13441 gattattgat atgaatcggt aatgatgcga ctcattacta ttccatttgt aataaagtac
    13501 gcagccctgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg gaaccacgga
    13561 caatgcccgc gccagcatat agtcgcacga cattgccact gatttttgct gagcgcaggg
    13621 aaacgcagaa ttcgctttgt tgtaaagaga gatagcccgc agaaccggcg taccattctc
    13681 gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt aagccagcaa
    13741 ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg ttgagtgaag
    13801 tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc ggcggtaaaa
    13861 catccagcgt ttgggtatcg gcctgtaatc gttggcagat atcttccacc accaacatgt
    13921 tctcacgctg gtttttatca tccgccatca gccactcacc caaccgctgc gcctgcttat
    13981 catcaggatg atttgctacc gttcccgcca gcgcttcagt acgcagcgct ttgtcacgcc
    14041 gccgccatag ccgttccggt gaagagccaa gaaaagcatt ttcgccatca aaggccatat
    14101 aaaaatgata gcagttcaga ttaagccgac gactggcagc catcatcgct gccgcattga
    14161 ccggacttgc gaaatgcagg tcagttgccc gagcaagcac cactttgtcg agctcacctt
    14221 cggcgatggt tttcgttgcc agttcgatta attgcgtcca ccccgttttg tccggccagt
    14281 gttgttctcg ggtggtggtt aaatgtaacc caggcaaggg cttgatactc accagtgtgg
    14341 cgataaattc ttttgcctta atcgcatcat gctgaaggga gctttcgctg aatagcgtca
    14401 gcctcagcgt ggctttaccg ccacagcgtc gccattccag gcgtggtaaa agtaaattgc
    14461 cctgcgacgg gtcaaacgca ttcagccccc agatgcgtaa gtcggcgtgt ttcgggtgct
    14521 gacgaagaaa cgtttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg cccaggacgg
    14581 cagcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt tgctgactgg
    14641 ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatca ataacccgaa
    14701 tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc agcgccgtag
    14761 taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg ggtactacaa
    14821 aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac agtcctagta
    14881 ccagcccaac ggccgcgccc acaccaattc cttgccaggg tttttcgtgg acgtagtcat
    14941 cagcacgata aacagcctgc ttcgcccgat agtaataact gtctgaagcc tggctaaccc
    15001 gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca tatttctgat
    15061 cggcgggatc gccagaggag cggagcacct cttccagtgt ttcactaagc agcgtcaggt
    15121 catcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat ttgcgtaaaa
    15181 cctgtcacta actatagaca agggttattg attacgcctg aattacctcg cgtgccatcc
    15241 caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaag ccaaaactct
    15301 ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggtttatca ggccagtgac
    15361 gcgtacatgt ttcgagtgtt ttattcatca gctgctgccc cactttttcg ccacgcaacg
    15421 cttcactaac aatcacccga ccaataacga ccggctcaag atcatcatca cttttcagaa
    15481 tcctcgcata cgccaccagt tcatcatttt tccaaccgag aatatggcga ttatcccccg
    15541 tcaaatcatc accatcgata tcctgataag ggcaattctg ctcaacaaca aacaccgcgc
    15601 aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga tgcagatctt
    15661 gccattcaat cataatgctc tccttttaga tgtatgcctt atactagccg gaatattttt
    15721 tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcagccggt gtgccaaccc
    15781 gtacgcgcaa tgtcacggca atattgctta acctgcaaca tccgacccag agcggtcttt
    15841 ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc tttaaccctg
    15901 gaaaactgga caagattttt atcagtcacc ttcatggcga tcatctcttt ggtttacccg
    15961 gcttgctgtg cagtcgttct atgtctggta ttgtccaacc cttaacgatt tatggtccgc
    16021 aaggtatccg tgaatttgtg gagaccacgc tgcggattag cggctcatgg accgattatc
    16081 cgctggaaat tgtcgaaatt ggcgctggcg aaattttcga tgatggcctg cgcaaagtga
    16141 ccgcttatcc gctggaacac ccactggaat gctatggcta tcgtattgaa gaacatgata
    16201 aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtaccgcct ggcccactgt
    16261 ttcaggaatt aaaagcgggc aaaacaatca tgctggaaga tggaaggcag attaacggcg
    16321 cagattactt agccgctcca gtgccaggta aagcgctcgc tattttcggc gataccggcc
    16381 cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt catggtgcac gaagcgacgc
    16441 tggatataac catggaagcc aaagccaata gtcgcggcca cagctctaca cgccaggctg
    16501 cgacactggc ccgtgaggct ggagtcggta agctaatcat tacccacgtc agctcgcgct
    16561 atgatgacaa aggttgtcag cacctgttac gtgaatgcag gtcaattttc ccggcgactg
    16621 aactggcgaa tgatttcacc gtgtttaacg tttaactctg cttattatta aaaacagggc
    16681 gaaacttgcc ctgttatcgc aacccgcgct acatatatcc ccccacacgg gcatattgag
    16741 cgatttttga tatttattca tctccttacg cactatattc agagcaataa gttgcctctt
    16801 tttatcttca ttcagcacaa cctttgcaac taaaaggagc ctatgatggt tacagttgtc
    16861 agcaattatt gtcaattatc tcaaacccaa ctctgtcaga catttgcaga aaaatttact
    16921 gtgaccgagg aattactgca gtctttaaaa aaaacagcgt tatccggaga tgaagaaagt
    16981 attgagttac tgcataacat tgcgttaggt tatgatgaat ttgggaaaaa agctgaagat
    17041 attctttacc atattgttag aaacccaaca aatgagaccc tatcgattat ccgacttatt
    17101 aaaaatgcct gtttaaaatt atataatttg gcacataccg caaccaaaca ccccctcaaa
    17161 tcacatgatt cagatgatct cctgttcaaa aaactattct ccccttcgaa attaatgaca
    17221 attatcggtg aggacattcc tcttatatca gaaaaacagt cgctttcaaa ggtactttta
    17281 aatgataaga ataatgaact gagcgatggt acaaacttct gggataaaaa tcgtcaatta
    17341 accacagatg aaatagcttg ctatcttaaa aagatagccg ctaatgcaaa aaatactcaa
    17401 gtcaattatc ctactgattt ctacctcccc aattccaaca gcacttacct ggaagtcgct
    17461 ctcaatgata atattaagag cgatccatta tggccgaaag cagtccagtt attccccata
    17521 aatactggcg gacactggat attagtttcg ctacaaaaaa tagttaatga aaaaaataac
    17581 acacaacaaa taaaatgcat cat
//
LOCUS       DAOTRK010000101        17571 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.101, whole genome shotgun sequence.
ACCESSION   DAOTRK010000101 DAOTRK010000000
VERSION     DAOTRK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.101"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            272..1171
                     /gene="yegS"
                     /locus_tag="QIE41_003198"
     CDS             272..1171
                     /gene="yegS"
                     /locus_tag="QIE41_003198"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDS3532053.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VRIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1253..2026)
                     /locus_tag="QIE41_003199"
     CDS             complement(1253..2026)
                     /locus_tag="QIE41_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDS3532054.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2132..3172)
                     /gene="gatD"
                     /locus_tag="QIE41_003200"
     CDS             complement(2132..3172)
                     /gene="gatD"
                     /locus_tag="QIE41_003200"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDS3532055.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKFFGAMQTFNSLEMSAP
                     QMQGVLRELRFNQLILETAGVPKTVELAVEIAGPHAQLALVGMLHQDLHLTSTTFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3220..4575)
                     /locus_tag="QIE41_003201"
     CDS             complement(3220..4575)
                     /locus_tag="QIE41_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDS3532056.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYLGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(4579..4863)
                     /gene="gatB"
                     /locus_tag="QIE41_003202"
     CDS             complement(4579..4863)
                     /gene="gatB"
                     /locus_tag="QIE41_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDS3532057.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4894..5346)
                     /gene="gatA"
                     /locus_tag="QIE41_003203"
     CDS             complement(4894..5346)
                     /gene="gatA"
                     /locus_tag="QIE41_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDS3532058.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDKDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5356..6618)
                     /gene="gatZ"
                     /locus_tag="QIE41_003204"
     CDS             complement(5356..6618)
                     /gene="gatZ"
                     /locus_tag="QIE41_003204"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDS3532059.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENANAA
                     MEKSVELVKAYVRAGFSKIHLDASISCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITRVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQEIIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMLNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6647..7501)
                     /gene="gatY"
                     /locus_tag="QIE41_003205"
     CDS             complement(6647..7501)
                     /gene="gatY"
                     /locus_tag="QIE41_003205"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDS3532060.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHGFDVSVEAELGQLGGQEDDVQVNEADALYTNPIQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7732..8784)
                     /gene="fbaB"
                     /locus_tag="QIE41_003206"
     CDS             complement(7732..8784)
                     /gene="fbaB"
                     /locus_tag="QIE41_003206"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDS3532061.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYLGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9041..10318
                     /locus_tag="QIE41_003207"
     CDS             9041..10318
                     /locus_tag="QIE41_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532062.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDITLTQGEVK"
     gene            10315..11319
                     /locus_tag="QIE41_003208"
     CDS             10315..11319
                     /locus_tag="QIE41_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDS3532063.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11316..12281
                     /locus_tag="QIE41_003209"
     CDS             11316..12281
                     /locus_tag="QIE41_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDS3532064.1"
                     /translation="MSGARLHTLLPELATRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12255..13001)
                     /locus_tag="QIE41_003210"
     CDS             complement(12255..13001)
                     /locus_tag="QIE41_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDS3532065.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSNVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13053..13871)
                     /locus_tag="QIE41_003211"
     CDS             complement(13053..13871)
                     /locus_tag="QIE41_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDS3532066.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQQVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFAHGDLPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(13936..14736)
                     /gene="thiD"
                     /locus_tag="QIE41_003212"
     CDS             complement(13936..14736)
                     /gene="thiD"
                     /locus_tag="QIE41_003212"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDS3532067.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14733..15521)
                     /gene="thiM"
                     /locus_tag="QIE41_003213"
     CDS             complement(14733..15521)
                     /gene="thiM"
                     /locus_tag="QIE41_003213"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDS3532068.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGRRTVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15556..15652)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15744..16016)
                     /gene="rcnR"
                     /locus_tag="QIE41_003214"
     CDS             complement(15744..16016)
                     /gene="rcnR"
                     /locus_tag="QIE41_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDS3532069.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16137..16961
                     /gene="rcnA"
                     /locus_tag="QIE41_003215"
     CDS             16137..16961
                     /gene="rcnA"
                     /locus_tag="QIE41_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDS3532070.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17180..17518
                     /gene="rcnB"
                     /locus_tag="QIE41_003216"
     CDS             17180..17518
                     /gene="rcnB"
                     /locus_tag="QIE41_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDS3532071.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLINDTDGKIIK
                     AYDGEIFYHR"
BASE COUNT         4329 a         4552 c         4253 g         4437 t
ORIGIN      
        1 ttccggataa ccccatgggg ggttaatata tttttaatag tatattgaag atgccactgt
       61 ttagttgaat attaggtata tgctcttttt tgaaatttac cggtggctac cgttaacatt
      121 cactgtcccc attgcaagct cctggtggta accactgaat cctctatact tgaactgact
      181 ttttatcctc cgactttcat cctgttctga ctccaccttt tcttttctgc tctacactat
      241 ctacagacca atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat
      301 tcttaatggc aaaagtactg acaatttacc attgcgcgaa gcaattatgc tgttgcgtga
      361 ggaaggaatg acgatccatg tgcgggtcac ctgggagaaa ggcgatgccg cacgatatgt
      421 agaggaggcc cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat
      481 taatgaagtt tctacggcgt tgattcagtg tgagggggat gacatacccg cgctaggaat
      541 tttgccatta ggaaccgcca atgattttgc caccagtgta aggattcctg aggcactgga
      601 taaggcgctg aaactggcaa ttgccggtga cgccattgcg atagatatgg cgcaggtcaa
      661 caaacaaacc tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga
      721 aacgccggaa aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat
      781 gcgcatggat actctgcaac cggaccgttg tgagatccgc ggtgaaaact ttcactggca
      841 aggtgacgcc ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcagcaatt
      901 gtgcccgaac gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga
      961 aattcttccg gctctcgtat caacattaaa atctgacgaa gataacccga atattatcga
     1021 aggcgcttcg tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg
     1081 cgaaccgttg agcggacaaa actttcatat tgaaatactt ccggcggcgt tgcgttgtcg
     1141 attaccacca gattgcccat tgttgcgtta atcacactat tctctcttga accagggaga
     1201 atccgtaatg ccggatgcgg tgtaaaaact ttatccggca tacagagcaa tactacaccg
     1261 taattaattt cacccgtgtc ttctgcaatg ccagtttatc cacctctgac acggcatcat
     1321 cggtaataat aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa
     1381 atttcgatga atcgagaacg gcaatcactt tatttgcggc tgtaaccatc gccccgctaa
     1441 tcgaataacc ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga
     1501 caaacattaa atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg
     1561 agcgtgtttt atggcggacg gtcccaccac agacgaccag agtaatgtct ttattttcgg
     1621 aaagggcaaa tgctgccggg agactgttgg taatcaccgt gatatttttg gcggtcatta
     1681 atccttcggc gataagcatt gtcgtacttc cgctgtcgag aataacggtc atcccttcgt
     1741 ggatcatttt gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc
     1801 gctctttaaa cccaacttcc tgggtctcgg tttcactatt accggacatt attttcgccg
     1861 caccgccatg aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga
     1921 ttgtcgcttc cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt
     1981 cattcactaa ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat
     2041 attcaattgt ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa
     2101 taccgggcca gcatcacgct ggcccgcggt ttcagggaat gagcaacact ttgcccggca
     2161 tagcattacg agcgatgtca cgcaccgcct gggcgaagct ttcaaagctt ccacggtgag
     2221 cgattaatgg ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact
     2281 cctgccccgg ccaagggctg gagtagttca tccagctgcc gataaccgtc agctctttac
     2341 gtaatatttt gccaaacgtt gtcgatgtta aatgcagatc ctgatgcaac atgcccacca
     2401 gcgccagctg ggcatgaggc ccggcaatct ctaccgccag ttcgacggtt ttcgggacac
     2461 cagccgtctc gaggataagc tgattaaagc gcagctcgcg taaaacgccc tgcatttgcg
     2521 gcgcgctcat ttcaaggctg ttaaatgttt gcatcgcacc gaaaaatttt gccagtgcca
     2581 gtttttctga gctaatgtcg atagccgtca cactctttgc tcccagcgcg acagcgcact
     2641 gaatagccag caggccaatg gttccggcac caataataat aacgttttta ttctcacaac
     2701 cttgcgctaa atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct
     2761 caataggcat atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag
     2821 caaatccacc atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc
     2881 ctttcaaaca ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc
     2941 caggatgtaa atcatcaaca ccggatccca cagcatcaat atagccgcta aattcatggc
     3001 ctaacgttat tggataataa tgtgcaccat ttttaaaaat tctgggtaaa tcggaaccac
     3061 ataatcccga gctggcaatt ttgacccgca cctcatcctg atgtttaatt tcaggaatga
     3121 cgctttctgc aacgcgcacg ataccatcag tatcattcac cactgatttc ataaaaactc
     3181 ctgattgtta agggggatta cctccccctc aggtaaaaat tattctgcga gaacggcttt
     3241 ctcttgttta ataaaaccac gcgctctacg ccaggtcatg aaaataccgg tcagataaat
     3301 tgcgccgata ataatgaaac cgggaatatt ttgcggggag aaaacctgaa tcagtaacca
     3361 ggtaatggga gaaccgccct gatccattga agccaccata cccccggctt ttaacgcccc
     3421 agcattagcc gccagttggg tgtgcaaacc aatagtttgc gtcgcgatcc acagggtgat
     3481 gctcataatg atgacacccg agattaaggt gcggaacaga tttccacgat gcacggcaac
     3541 cgccatcgcc acgaagaagc cgatagttgc aagatcgcca aacggcagca cctgattacc
     3601 cggaacacaa acagcaatta aaatggtgag agggataaaa atcaggcttg ccgataccac
     3661 cgccgtatgc cccagcagca acgccggatc aaggccaatc aggaactcct gaccgccgaa
     3721 cttcgcctgt aaacgactac gggcctgctt agcgataggc gttaaaccat ccatgatggg
     3781 tttaatcact cttggcatca gcagcataac tgccgccgtt tttaccgcca gctgcaatac
     3841 acctttgaca tcgtaacccg cgaggatgcc gataatcagc cccatcacaa aacccacggt
     3901 gacaggctcg ccaaacggac caaatttgcg ctgaatatcg tcggcgctaa atttaatacg
     3961 gttaacgcct gggattttct cgatgatagc atccaccagc accgcaattg gccccagata
     4021 cgccgacgta ccgtgcggaa tagcaatgcc ttccagctca aagaaatttc gggtatcgcg
     4081 ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga attaccacac ccgccatccc
     4141 tatcatccat gaaccggttg ccagatgcag caatgcgccg gtgaaggtca tatgccagat
     4201 attccagata tcaacattta ccacccgcgt catacgggtc agtagcatcg ccacgttaac
     4261 cagaatcgca atcggaatcg ccaccagcgc aatttgcgac gcccaggtca ttggtgaaga
     4321 gcccggccaa ccgacatcga ccacatgcag attcaggtcg aaattttccg ccatcgcttt
     4381 cgccgccgga ccaatggaat ccagcattaa gccaatcaca aggccaatgc caacaaagcc
     4441 aatcccgata tgcagacccg ctttaaagca atcgcctgcc ttcatgccta atattttaga
     4501 aaaaataatg atgacaatcg gcagcatcac cgtagggccg aggtcgagaa tataacgcat
     4561 gacttctgaa aacataggtc acccctgtaa gatagtcaga attttatttt gtaatgcttc
     4621 gataccgaca ccagaaacaa aaggcatgcc gtgaactaac ggaatatcgc caaaactacg
     4681 gtcaactttg gcggtagtac atatcaaatg cacaccatcc atataggttt ctatttcatt
     4741 aacccgacac tggattaatt caacaggaat attatgactc tgacacaact ctttaatttc
     4801 ttccgccgcc atcgtagagg tcgcaaccgc gcctccgcaa gcgacaataa tcttgcgttt
     4861 cataaatagt cctttttatt gttacagagg gatttattcg tctgaatcta aaacatactt
     4921 tgtgaagtat tcctttaact gggtttcagg aagagtgatt agtgtctcga cgatatcggg
     4981 ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat tgctgctgcg gattttccac
     5041 aatcaacgcg ataaccaacg ataccgccac gtccttatca tcatccgctt gctgaaaatg
     5101 aactttattt gttggcctta acagataaat ggctgacgac ttagcatgaa tcgcctcaca
     5161 atgcggtatt gcgatggcgt gctgctcaag cattatcccg gtagggaatt ctgcttctct
     5221 ggcaattaat gcctgtggcc aggtatcatg aaccacacct ttcgcgagca tctcattacc
     5281 gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt ccgctacgaa caaacaggtt
     5341 agtcatatac cgtccttatt ccgcacagcc gtagcgatag gcgcgcaaaa catcataaat
     5401 tttatccata atcagctgat gcggtattgc tgataattcc ccggactgaa tgcgttcaaa
     5461 ttgtttgggg agatactgac taatcatgcc cagtgggatg tccacgcctt gcagattcaa
     5521 catcatcgtt tcgacgctat ttttaatccg actatgcggc cagtaataac gaatacgatc
     5581 cgacaggctg taacgaatat ccagtaatga atcgttaaaa cccgtacgat aatatttttt
     5641 ccagtattgc ggttcgtcga gcatcacttc ttcaattaca gccaggcaac cgctgcgatt
     5701 ttcgggggcg ataatttcct gctcaatttg tgctaacgca aatatcgcct cacgtagagc
     5761 aaaggttaat gctggcccga ctttcaaaat tgcaaagtga tcgcggacta attcccaata
     5821 agccgtccgg gtctggtaat cggtagaatg tgcttcataa accattcggg tgttttctat
     5881 ccattgcgcc agcgcctgcg cttcttgcgg ctgataatgg ataatattgc tgtgatcaaa
     5941 ttccacaccc ggctgcacca cgatggcaat cacgcgtgtt aacgcctctg tcagcccacg
     6001 agcaataaag gccttttgat gcgtacgtaa agtattggcg gcatcttcaa cccgggtaat
     6061 gtgtactgac tgaatggcgc tggcctcacc gcccggaacc ggcacttcgg tgccaatgac
     6121 atagctcagt tgctcacgct ggcaatctgt cgccacactt tccgcagcaa agcaaagcac
     6181 agcagctcgt tccgcaaccg tttctggtgc taacggtatg ggatcccccg cgcaggatat
     6241 tgacgcatca agatgaattt tactgaagcc agcacgaaca tatgccttta ccagctcgac
     6301 ggatttttcc atcgccgcat tcgcattttc ttgctgccag cagtttggcc ccagatgatc
     6361 gccgccgaga ataatgcgct cgcgcgcaaa tcccacttta tcggcaatcg taaaaacaaa
     6421 ttcgcgaaag tctgccggtg tcattccggt ataaccgcca aattgattga cctggtttga
     6481 cgttgcttca atcagcactt tgcgcgtgct gttgcgatca aatgccagcg ccgcttcgat
     6541 aaccaacgga tgggcagaac agactgagca tatgccgata tgttcaccag ctttatgccg
     6601 ggcaattaac gttttcatgt tttttccttg ttaaatggca ggtgcgttat gccctgccct
     6661 cgcagccaca atcggcaatc actttgctca ccacatcgcg cattgcggat ttagccgact
     6721 gcaaataatc ccggggatcg gtcgcttcgg ggtgctcggt caggtaattt ttcaacgcct
     6781 gcgagaaggc atttttcagc tccgttgcaa cgttgatttt gcatatcccc agtttgatgg
     6841 tttgctgaat atccttagtc gataaccctg acgcgccatg cagcaccagc ggtaagttca
     6901 cccactggcg aatgttctcc agccgagaaa aatcaagcgc cggtgcgctg gcatacatac
     6961 catgagccgt gccgatggcg accgccaggg aatcaattcc ggttgcctcg gcaaattcac
     7021 gcgcctgaat ggggttggtg tacaacgcat cggcttcatt gacttgcaca tcatcttcct
     7081 ggccgccaag ttgccccagc tccgcttcga cgctgacatc aaagccatgg caaaaatcca
     7141 ccacctcttt gacccgtgaa atattttgcg caaaaggcaa atgcgaggcg tcaatcatga
     7201 ctgagcgcac gccagaacga accttctgag cgatatcgtc aaatttcgtg tgatggtcga
     7261 gatgaatcgc cagtggatgg tgatattgct tcgccatcgc gctgaccagc gccaacaaat
     7321 tttctgtacc tgcatgagta aatgtgccgg gcgttccggc gatgatgacc ggcgcatgca
     7381 tgctggcagc ggtttctacc accacttgca tcgtttcgag attgtgaata ttgaatgccg
     7441 gaaccgcata accgccgcgc tgcgcgttgt tcagcatctg ctttgtcgat accacgtaca
     7501 ttttcatatc ctgtcgtttg ctttcgattt caaaatataa tgaaattatt tgtttttaaa
     7561 tgtcgagata acgatcacaa aaacgacaat atgaaaattg ttcgaggagt gaaaggcaaa
     7621 aaaaacggcc tcccgatagg gaagccgtag caaagtgcgc gttattttat gccggatgca
     7681 gtgtaaacgc cttatccgtc atacgtggca gtgctggtcg tacaaagcac atcaggcgat
     7741 agtaattttg ctatcgagat aaacgtcctg cacggcgtta atcagtttca cgccgtcagc
     7801 catcgatttc ttgaacgcct tacgtccaag aatcagcccc attccgcctg cgcgtttgtt
     7861 gataaccgca gtacgcactg catcgctgag gtcagtttca ccgcctgcag caccgccgga
     7921 gtttatcaac cctgcgcggc ccatatagca gttagctaac tgataacgca ccagatcaat
     7981 cgggttttcg ctggtcaact tgctgtacac gcgatcgtcg gtataaccgt aattaatcgc
     8041 tttatagccg ccgttatttt ccgccatttt ttgtttaacg atatctgcac ctatggtcgc
     8101 cgccagatgg tttgcctgac cggtcaggtc ggcggaaaca tggtaatcaa cgccatcttt
     8161 cttaaaggcg gagttacgca aataggccca cagcactgtc accatgccca gctcgtgcgc
     8221 acgttcaaaa gccgcagaaa tttcttcaat ctggcgacgt gactcttccg aacccaaata
     8281 gatagtcgca ccaaccgcca ccgcgcccat gttgaaggcc tgctccacgc tggcatacag
     8341 cgtttgatcg taggtattcg ggtaacttag cgtctcgttg tgattaagtt tgacgaggaa
     8401 tggaatgcga tgcgcgtaac gccgcgatac cgatgccagc acgccgtaag ttgacgccac
     8461 acagttacag cccgcttcga tcgccagttc aacaatgttt ttcgggtcaa agtagagcgg
     8521 gttagcagca aatgaagctc cagcagagtg ctcaacgccc tggtcaaccg gcagaataga
     8581 aagatatcct gtgccagcca gacgcccggt gttgtacaac gtctgcatat tacgtaacac
     8641 cgctggcggg cgattattgt caatcatcac gcggtctacg taatcatgcc cggggagata
     8701 aagctggtca gaaggaatag tcatacaacg gtgctgtaaa aggttgtcgg cgtctttgcc
     8761 aagcaactgc gcaatatctg tcattactat gctcccgtaa attccgattg gatatcggct
     8821 atggattgtc ctggcccgcc ttttgcgggc aatcataatc ctggtcgtta caggcgagtt
     8881 tttccatctt ttggatgttt tttcagcgtt ttctgttggc tcgattcatc agaaaaaatg
     8941 ttaccgcggt caagttttca ccgcaaaggt atttaaaagg tattattaaa tggtattgtc
     9001 atcgcgtacc ttacattacc tgtcatgaag gaattaaaag atgaaaacaa cagcaaagct
     9061 gtcgttcatg atgtttgttg aatggtttat ctggggcgcg tggtttgtgc cattgtggtt
     9121 gtggttaagt aaaagcggtt ttagtgccgg agaaattggc tggtcgtatg cctgtaccgc
     9181 cattgcggcg atcctgtcgc caattctggt tggctccatc actgaccgct ttttctcggc
     9241 gcagaaagtg ctggcggtat tgatgttcgc tggtgcggcg ctgatgtatt tcgctgcgca
     9301 acagaccact tttgccgggt tcttcccgtt actgctggcc tactcgctaa cctatatgcc
     9361 gaccattgcg ctgactaaca gtatcgcttt tgccaacgtg ccggatgtgg agcgtgattt
     9421 cccacgcatt cgtgtgatgg gcactatcgg ctggattgcc tccggtctgg catgtggatt
     9481 tttgccgcaa atgctgggtt atgccgatat ctctccgact aacatcccgc tactgattac
     9541 cgccggaagt tctgctctgc tcggtgtgtt tgcgtttttc ctgcccgaca cgccgccaaa
     9601 aagcaccggc aaaatggaca ttaaagtcat gctcggcctg gatgcgctga tcctgctgcg
     9661 cgataaaaac ttcctcgtct ttttcttctg ttcattcctg tttgcgatgc cactggcgtt
     9721 ctattacatc tttgccaacg gttatctgac cgaagttggc atgaaaaacg ccactggctg
     9781 gatgacgctc ggccagttct ctgaaatctt ctttatgctg gcattgccgt tttttaccaa
     9841 acgctttggt atcaaaaagg tattattact tggtctggtc accgctgcga tccgctatgg
     9901 cttctttatt tacggtagtg cggatgaata tttcacctac gcgttactgt tcctcggcat
     9961 tttgcttcac ggcgtaagtt acgattttta ctacgttacc gcttacatct atgtcgataa
    10021 aaaagccccc gtgcatatgc gtaccgccgc gcaggggctg atcacgctct gctgccaggg
    10081 cttcggcagt ttgctcggct atcgtcttgg cggtgtgatg atggaaaaga tgttcgctta
    10141 tcaggaaccg gtaaacggac tgactttcaa ctgggccggg atgtggactt ttggcgcggt
    10201 gatgattgcc attatcgccg tgctgttcat gatttttttc cgcgaatccg acaacgaaat
    10261 tacggctatc aaggtcgatg atcgcgatat tacgttgaca caaggggaag ttaaatgaaa
    10321 acagaacgta ttctcggtgc tctttatggg caggcgttag gggatgcgat ggggatgccc
    10381 tccgagcttt ggccgcgcag tcgcgtcaaa gcgcactttg gctggattga ccgttttctg
    10441 cctgggccaa aggagaataa cgcagcctgt tattttaacc gcgccgaatt caccgacgat
    10501 acctcgatgg cgctgtgtct ggcggatgcg ttactggaac gtgaaggcaa gatcgatccg
    10561 gatctgattg ggcgtaatat tctcgactgg gcgctgcgtt tcgacgcctt taacaaaaac
    10621 gtactaggtc cgacctcgaa gattgcgctt aacgccattc gcgacggtaa acccgttgct
    10681 gaactggaaa ataacggcgt gaccaacggc gcagcgatgc gcgtctcgcc attaggttgt
    10741 ttgcttccgg cgcgtgatgt tgattccttt attgatgatg tggcgctggc ctccagcccg
    10801 acacataaat ccgatctggc ggttgcgggg gcggtagtca tcgcatgggc gatttctcgt
    10861 gccattgacg gagaaagctg gtcagcgatt gttgattcac taccctcgat tgcgcgacat
    10921 gctcaacaaa aacgcatcac taccttcagc gcctcactgg cagcacgtct ggagattgcg
    10981 ctgaaaattg tgcgcaatgc cgacggcacc gaatccgcca gcgaacagct ttaccaggtc
    11041 gttggcgcag gtaccagcac tattgagtcc gttccgtgcg ccattgcgct ggttgaactg
    11101 gcacaaaccg acccgaaccg ttgcgccgtc ctgtgcgcta accttggcgg cgacacagac
    11161 accatcggtg ctatggcgac agcaatttgt ggcgcgttgc atggcgttaa cgctatcgat
    11221 cctgcgttaa aggcggaact ggatgcggta aatcagcttg atttcaaccg ctatgccaca
    11281 gcgctggcga aatatcgtca acaacgggag gcggtatgag cggcgctcga ttacacacgc
    11341 tgctgcctga attagccacg cgtcagccag ttatggtgat tggcgcggcg gtcattgatg
    11401 tgatcgccga cgcttatgcc ctcccctggc gtgggtgcga tatcgaactg aaacagcaga
    11461 gcgttaacgt tggcggctgc gcactgaata ttgccgtggc gttaaagcgc ctcggcatcg
    11521 aagcgggtaa tgccttgccg ctcggtcagg gggtttgggc ggagattatt cgcaaccgga
    11581 tggcaaaaga gggcttaatc agcctgatcg ataacgccga aggtgataac ggctggtgtc
    11641 tggcgctggt tgagccggat ggcgaacgca cttttatgtc attcagcggt gtggaaaatc
    11701 agtggaatcg ccagtggctg gcgcgattaa ccgttgcgcc tggcagcctg ctctattttt
    11761 ccggttatca actggcctcg ccctgcggcg aattattagt ggaatggctg gaaaagctgc
    11821 aagacgtgac gccgtttatc gattttggcc cacgtattgg cgatatcccg gatgcattgc
    11881 tggcgcggat catggcctgt cgacctttag tgtcactcaa tcgtcaggag gctgagattg
    11941 ccgccgaacg ttttgcttta tccgcagaga taacaacact tggcaagcaa tggcaggaga
    12001 aatttgccgc gccgttgatc attcgtctcg ataaagaggg cgcatggtat ttcagcaacg
    12061 acgcttctgg ctgcattccg gcatttccaa cgcaagttgt agacaccatt ggggcgggcg
    12121 acagtcatgc aggtggcgta cttgccgggc tagcctctgg tctgccactg gcggatgccg
    12181 tattactggg caatgcagtg gcgtcgtggg ttgtcgggca tcggggcggt gattgtgcgc
    12241 caacgcgcga ggaactactc ctcgcacaca aaaacgtata gatcgctgcg acagtggctg
    12301 atgctgtact caataggccg ttgctgttgg tcaagcgcca cttgcttgat caccagcacc
    12361 ggtattttgc tatctaactg aatatgtgac tgaaactcgg catccggcat ccgggcgcta
    12421 acgcgggaac gcgtacgttg tgggtaaatg tgctggctgc ggaagtagtc ataaagagaa
    12481 attccgatgg catcaacatc atgaattaaa tgcgcaggaa cccacgattc ctcaattgat
    12541 accgcttctt catcgacata gcgaatacgc ttaagcaaaa agacattact tcctgcttcg
    12601 accgctagct gctgcgcgac ttcttcggga catttcacga cacgcttgtt aacccatagt
    12661 gtatcgggct ttttcccgcg caataccacc tgctgagaaa aacctcgcgc ttctttcaac
    12721 gagtattcga agatgttgtt gatctgcgta ccgtaaccac gcgaacgcgt caccacgccc
    12781 tcttcttcca gcgcctgcat cgccttgcgc accgtaatgc gcgaaacgcc ggttaactga
    12841 cttaaatcac gctcaccggg caaaatattg ccatgctcca gcaccccgct gcgcacggca
    12901 ttttttaccg tttcggcaaa cttaatataa agcggcgtgt tatccgccgc taaaatacgt
    12961 tcgttcagtt gggcaatcag ctgggtatgc gcttgttcca tttgtctttt tcctgccagg
    13021 gagtctggtg ggagtatatc actgcttctt atttaaggcg tttctttaat cccatcaacg
    13081 aatgcctgca actcctccac cgtgccatta aacacattaa aatccaccgg accattaatg
    13141 ccatctacct gtccacggtc ggaatgctgc cagaagcgcc aggccatgcc gtcattgtcc
    13201 gggcgacgtt gataatagtg agccacccac cacggatatt cattgaaata gcccgccaga
    13261 ttggtgtgat aaaaaacggc tcctgagtaa ataattggct tttttcccgt gcgtttttcg
    13321 accattttta gccactgact tacccgcttg cgtaattctt ttgccgataa ttttccccgt
    13381 tcttctacgt ccagcacggc agggagatcg ccgtgggcaa aatccaccgt ttgcagaaat
    13441 aatcgcgcct gaactgaagc ggataccgac ggggagaaat aatgatacgc cccgcgcagc
    13501 aggccatttt cgcggcttag ttgccagtta cgcgaaaaat aggggtccac cagcttttcg
    13561 ccttccgtcg ccttgataaa ggcaaactgc aagcgaatac cgttatcgcg catttttgcc
    13621 acctgctgcc agtcgattcg ctcctgccag cgtgaaacat caataccatg aatggtgtaa
    13681 ctggcaggaa tgcgaatagc aaaagatttg accggacgat aaccgtagaa gtggatgtag
    13741 tctttaatct gaattgctgt cgagtagaaa aaattcaccg tctgacgcgg ataaattgca
    13801 acgatggaaa ttagcccaag agcgcataat aaagcggtga atttttttcg actggtgatt
    13861 ctcagttgca ttggtttcat ccctgaatgt cagcgccaaa gactgacaat aaccaaagca
    13921 gctatagtaa ggtgctcacc accacgcgtg gaagtggtga accggaccaa taccgtgacc
    13981 aacttccagc gtgtcggcct gggctaacgc cgatgaaagc cagctttttg cctcctgtac
    14041 ggtgtcagcc cagtttgtat ggcgcgggcg tagtgcagcc aacgccgcag agagcgtaca
    14101 accagtgccg tgggtgtttt tggtcataat gcgcggtgca gtaaatcgtt gttctccctc
    14161 acgagtaaac agccagtccg ggctttgttc atcatccaga tgaccacctt tcattagcac
    14221 tgcgccacag cccatcgcca acagcgatcg cccttgttcc agcatttcct gttcggtgcg
    14281 cgcgtgtggc gcgtcgagca aggcagcagc ttcgggcaag tttggcgtta ttaatgaaac
    14341 ctgtggcaat aatcgactgc gcagcgtagc aaccgccgaa ggtgaaagca gcgggtcgcc
    14401 gctttttgcc agcataacgg tgtcgagtac cacgttttgg atctgataac gttgcaaccg
    14461 ttctgccacc gcttcaacaa tatcggtttc cgccaacata ccgattttag tggtgtcgat
    14521 tcgcacatcg ctgaacaccg aatcgagctg ggcggcgaca aaatcaggct caatgcgata
    14581 caccgactgt acaccacggg tattttgcgc caccagtgca gtaataactg agcaaccata
    14641 agcgccaagt gccgagaagg ttttaagatc ggcctgaatc cccgcaccac cactcggatc
    14701 agtaccggca atcgtcagag cgttaattcg tttcacgctt gcacctcctg cgtcaattgc
    14761 cagagcgcat caaggaaatg tggaacaaaa ctgcctggcc cctcgctact ggcgactgcg
    14821 cgttctccgg cttgtttcat ccagtgacag gcagatgcga tattttccag cgtatcgcct
    14881 ggtagcgcac aacaggcagc gacaaccgcc gataatgcac agccagttcc taccactttg
    14941 gtcattaacg gatcgccgcc gtgaatgcca acggtgcgac gaccatcggt aacataatcc
    15001 acctcgccag tgaccacgac gattgcgcca gtttcccgtg ccagtgtttg tgcagcgggt
    15061 atcgcattag ctgcggcgtc agtggtatcc actccccgtc cgccattagc aatgccagct
    15121 aatgccatga tttccgaagc attaccacgt atggcggttg gcttatgaga cagaagttcc
    15181 agacaaaaac ggcggcgata gtcgagtgca ccaaccgcaa ccggatcaag cgtccagggt
    15241 gtttgagagc gttttgcttg ctcaacggcg gcacgcatcg cctgtgcgcg tggctgcgtc
    15301 agtgtgccaa cgttaatcag caacgcactg gcgatagccg caaactgact ggcctcttcg
    15361 gtttcgataa ccatcgctgg cgatgcaccg agcgccagca aggtattggc agtaaaggtt
    15421 tgcaccacat cgttggtcat gcagtgcaca agaggggaat gttggtgaaa aaggtgtaac
    15481 gcgtgcgcag attgcgcgga actcagcagg tcgacttgca tagtttgctc ctgccataac
    15541 gtgaagaagc aatgacctgg tggtccgtga cttccctacg ctggcattat ccagatcagg
    15601 tgatacgggt atttctcagc cttcacgcag aagggcaccc cgagtcgttt gattgcgatg
    15661 ataaatcgca gaggaggatg gtaatgtcca gcgcacgcgt tgtaaacggg gaaaaacggc
    15721 agttttacaa tcgcgttaat aaattatttg atataagaat ccagcacctt cagaacgaca
    15781 tccagatctt cttcacgttt tagctcatcc ccctggtgaa cgatgtgttc cgtcagatga
    15841 cctttaatca cttcccgcat cagaccgttt accgcgccac ggatagcagc aatctgttgt
    15901 aaaactgcag cgcattcgtg cggctcgtcg agcattttct tgagcgccac gacctggccc
    15961 tgaatcttac tggcacgcgc tttcagtttc tgtttatcac ggatggtatg agacatggca
    16021 acacctggtt aacaagaata tgaaaaatca tagcactatt aatctactgg ggggtagtat
    16081 caggtactgg gggggagtag aataagattg ccgaattaat actaagaatt attatcatga
    16141 ccgaatttac cactcttctt cagcaaggaa acgcctggtt cttcatcccc agcgccatct
    16201 tacttggtgc gcttcatggc ctggaaccag ggcactcaaa aacgatgatg gcggcgttta
    16261 tcatcgccat caaaggcacc attaaacagg cggtgatgct cggactggca gcaaccattt
    16321 cgcataccgc agtggtctgg ttgattgcct ttggcgggat ggtgatcagc aagcgcttta
    16381 ctgctcaatc agcagaaccg tggctccagt tgatttccgc agtgatcatt attggcaccg
    16441 cgttctggat gttctggcgt acctggcgcg gcgaacgcaa ctggctggag aatatgcacg
    16501 agcatgatca tgagcatcat caccacgatc acgaagatca ccacgaccat ggacatcatc
    16561 accatcacga acatggcgag tatcaggatg cccatgcacg agcccatgcc aatgacatta
    16621 aacgacgctt tgatggtaga gaggtcacca actggcaaat tttgttattt ggcttaaccg
    16681 gtggccttat cccctgcccg gcagcaatta ccgtgctgtt gatttgcatt cagttgaaag
    16741 ccctgacact gggcgcaaca ctggtcgtca gtttcagcat tggcctggcg ttaacgctgg
    16801 tcaccgtagg cgttggcgca gcaatcagcg ttcagcaggt cgcaaaacgc tggagcggat
    16861 ttaacactct cgctaaacgc gccccctatt tttccagtct gttgattggc ttagttggtg
    16921 tgtatatggg cgtacatggc ttcatgggca taatgcgata actcaatcct ttaggcttgc
    16981 atgttatctt cagcccagga tgattcttaa atcagctatt tccgctgact ctctgctcgc
    17041 taaggatgct tttagggcat cctttcattt acacttttta cgaaatcatg ggatcactaa
    17101 caaaatatcg cttgtcagtt atattgtatg gcaggaaaga tatgcgactg atattacaga
    17161 tccccaaagt ggagagttta tgaccattaa aaataagatg ttgctgggtg tgcttttgct
    17221 ggttaccagt gccgcctggg ccgcaccagc caccgcgggt tcgaccaata cctcgggaat
    17281 ttctaagtat gagttaagca gtttcattgc tgactttaag catttcaaac caggcgatac
    17341 cgtcccagag atgtaccgca ccgatgagta caacattaag cagtggcagt tacgtaacct
    17401 gcccgcgcct gatgccggga cacactggac ctatatgggt ggcgcgtacg tgttgatcaa
    17461 cgacaccgac ggtaaaatca ttaaagccta cgacggtgag attttttatc atcgctgaaa
    17521 aaaagccccc tcgaagagag ggggaaatgc agacacctta ttatttttat t
//
LOCUS       DAOTRK010000102        17519 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.102, whole genome shotgun sequence.
ACCESSION   DAOTRK010000102 DAOTRK010000000
VERSION     DAOTRK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.102"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..725)
                     /locus_tag="QIE41_003217"
     CDS             complement(<1..725)
                     /locus_tag="QIE41_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3532072.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDVKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVDENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGKMRWVAEIYGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLGEAYL
                     PGVDMRDCYFGKKTIIDKKALYLEPGWNANLDGATLDGAILDGATVDGATRLYAEVII
                     INKITPKKIVTEGVATKQSTAEK"
     gene            complement(1020..1835)
                     /gene="djlA"
                     /locus_tag="QIE41_003218"
     CDS             complement(1020..1835)
                     /gene="djlA"
                     /locus_tag="QIE41_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDS3532073.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            2090..4444
                     /gene="lptD"
                     /locus_tag="QIE41_003219"
     CDS             2090..4444
                     /gene="lptD"
                     /locus_tag="QIE41_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDS3532074.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAADTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            4497..5783
                     /gene="surA"
                     /locus_tag="QIE41_003220"
     CDS             4497..5783
                     /gene="surA"
                     /locus_tag="QIE41_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDS3532075.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5783..6772
                     /gene="pdxA"
                     /locus_tag="QIE41_003221"
     CDS             5783..6772
                     /gene="pdxA"
                     /locus_tag="QIE41_003221"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDS3532076.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPTSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6769..7590
                     /gene="rsmA"
                     /locus_tag="QIE41_003222"
     CDS             6769..7590
                     /gene="rsmA"
                     /locus_tag="QIE41_003222"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDS3532077.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            7593..7970
                     /gene="apaG"
                     /locus_tag="QIE41_003223"
     CDS             7593..7970
                     /gene="apaG"
                     /locus_tag="QIE41_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDS3532078.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7977..8825
                     /gene="apaH"
                     /locus_tag="QIE41_003224"
     CDS             7977..8825
                     /gene="apaH"
                     /locus_tag="QIE41_003224"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDS3532079.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(8822..9123)
                     /locus_tag="QIE41_003225"
                     /pseudo
     CDS             complement(8822..9123)
                     /locus_tag="QIE41_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
     gene            complement(9126..9359)
                     /locus_tag="QIE41_003226"
     CDS             complement(9126..9359)
                     /locus_tag="QIE41_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDS3532080.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(9445..9924)
                     /gene="folA"
                     /locus_tag="QIE41_003227"
     CDS             complement(9445..9924)
                     /gene="folA"
                     /locus_tag="QIE41_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDS3532081.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(10116..11978)
                     /gene="kefC"
                     /locus_tag="QIE41_003228"
     CDS             complement(10116..11978)
                     /gene="kefC"
                     /locus_tag="QIE41_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDS3532082.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11971..12501)
                     /gene="kefF"
                     /locus_tag="QIE41_003229"
     CDS             complement(11971..12501)
                     /gene="kefF"
                     /locus_tag="QIE41_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDS3532083.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(12609..13940)
                     /locus_tag="QIE41_003230"
     CDS             complement(12609..13940)
                     /locus_tag="QIE41_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532084.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMSKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13999..14286)
                     /gene="fixX"
                     /locus_tag="QIE41_003231"
     CDS             complement(13999..14286)
                     /gene="fixX"
                     /locus_tag="QIE41_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDS3532085.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(14283..15569)
                     /locus_tag="QIE41_003232"
     CDS             complement(14283..15569)
                     /locus_tag="QIE41_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS3532086.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTN
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(15620..16561)
                     /locus_tag="QIE41_003233"
     CDS             complement(15620..16561)
                     /locus_tag="QIE41_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDS3532087.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMVEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(16576..17346)
                     /locus_tag="QIE41_003234"
     CDS             complement(16576..17346)
                     /locus_tag="QIE41_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDS3532088.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         4336 a         4859 c         4324 g         4000 t
ORIGIN      
        1 aatttttcag cagtactttg tttagtagca actccttcag taacaatttt ttttggggtg
       61 attttattaa taataattac ctcagcatat aggcgggtag caccatccac ggtagcaccg
      121 tccaggatag caccgtccag ggtagcaccg tccaggttag cattccaccc aggttccaag
      181 taaagggctt ttttatctat tattgttttt ttaccaaagt aacaatccct catgtcaacg
      241 ccgggaaggt aagcctctcc aagatcaacg cccaagttac ctgggccttt atgcagctgc
      301 aacgtctgag tacatatctc cttattttca atgaaaaaag tgacacttaa ctcgatatca
      361 ccgtagatct cggcgaccca gcgcatcttg ccctttgcga ccatctcatc cggatttttt
      421 gcgcatgttt tgagttcatt gaataattcc agactatgga taaacttttc tttctcattc
      481 tcgtcgacag ccccagtaaa gtattttgag gcgttgatgt tgccagcctt tttattcacg
      541 aaattgaaca gaagtttata agcctcagtc tgtttatctg accagaacag atgacgtaac
      601 gtctcccaca agcgttgaaa gatattcatc ttcacgtcca tcttgtcccc gctcaccatc
      661 ttataaatat cgttattgct catatttaaa agtgtagccg gcatgcctgg aactgatact
      721 ttcatggtaa tagcctcgca ttgagttaaa aactcatgat gcgcgctctg aataaatata
      781 ctttcaaaaa tcgctcattc agatatgtgt tttttatttt taagacgtaa aaatataatt
      841 ccgctcgtcg taaagctctc aaccttaagc agggtcaacg cgataaaacc aacagaaact
      901 ttcccataaa ccgtgtattg agcctgtcca ccactctgag taaacgcctt ttctcaaaga
      961 agtgaccgcc agggcggcca cctttaatcg tgggcagtta ctcagcatca tttacagggt
     1021 catttaaacc ctttctgctg ttttatcagc tcataggcct gctgaatttc ctgcgctttc
     1081 tgcttcgcca tctccatcat ctcaggcggc aaacctttcg ccaccagctt atcgggatgg
     1141 tgttcactca tcagcttacg gtaggcacgt ttgatggtgg tcgcatcatc cgtcggcttc
     1201 acgcccagca cattacaggc atcttccagc gtcgggccac gctgcgcttg ctgccagtta
     1261 ccaccgccag attgctgctg ataaccgccg ccaaactgtg caccgccctg catcatgcgc
     1321 aaaaactggt caaactgggc gcgggagatg cctaattctt cagcaataac atacagcacc
     1381 gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg cctgaatctg gatctccaga
     1441 aacatacgaa ttaagtcaaa acgaccaaag caaacactgc gaaactggcg catcttttcg
     1501 cgcagcgggt aattgtctga ttttcccacc cggaacgcat tttgcgccgc agtacgggaa
     1561 gcgccatgaa ggttcattcg gtccatcaac tggctggcga tatgaatatc agcctccgtg
     1621 acacgacctt tggatttggt taaatggccc atcacttcaa aagtggtggc aaaaaacagc
     1681 gcctgacgct cacgctggtt ggcaaaccac gccattttac ggctacgggc tttatcaaac
     1741 atatggccaa ttaacaggcc taacactacg ccccaaaagc cgccgcccat cagtaaggcc
     1801 acggccacgc caatgatttt tccccaatac tgcatatatt ccccaaatcg acacacggat
     1861 atcagggcta tctcccacaa tataaaggtg cttttaccgt tttccggctg cggtcaattg
     1921 tgggacatcg cctataattt gcattatcat acctgtcatt caatgccgtg cctaacacca
     1981 ctgacgctat tcggacagga ttaacactag cgtagagatg acgagtacgt tagtctctga
     2041 ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa aatcaacgta tgaaaaaacg
     2101 tatccccact ctcctggcca ccatgattgc caccgccctt tatagtcaac agggactggc
     2161 agccgacctc gcctcacagt gcatgttggg cgtgccaagc tatgaccgtc ctctggtaca
     2221 gggcgatacc aatgacttac ccgtgactat caatgctgac cacgcgaaag gggactaccc
     2281 ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag ggtaacagcc gtctgcaggc
     2341 cgacgaagtg cagctccatc aaaaagaggc accaggacaa ccggagccgg tacgtaccgt
     2401 tgatgcgctc ggtaatgtcc attacgacga taaccaggtg atcctcaaag ggccgaaagg
     2461 ctgggcgaat ctgaacacca aagataccaa tgtctgggaa ggtgattacc agatggtggg
     2521 tcgtcagggc cgcggtaaag cggacctgat gaaacaacgt ggcgaaaacc gctataccat
     2581 tctggataac ggtagcttta cctcctgtct gccgggttct gacacctgga gcgtggtagg
     2641 tagcgaaatt attcatgacc gcgaagaaca agttgcggag atctggaacg cccgctttaa
     2701 ggtgggtccg gtaccgatct tttatagccc ctatttgcag ttgccggtgg gtgacaaacg
     2761 tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc accaactact ttgagttcta
     2821 ccttccatat tactggaaca tcgcgccaaa tatggatgcc accatcacgc cgcattatat
     2881 gcatcgtcgt ggcaatatca tgtgggagaa cgaattccgc tacctctccc aggcgggcgc
     2941 tggcttgatg gaactggact atctgccttc agataaagtc tatgaagatg aacacccgaa
     3001 cgatgacagt tcacgtcgtt ggttattcta ctggaaccac tccggggtca tggatcaggt
     3061 gtggcgtttc aacgtcgact acaccaaggt cagcgatcct agctacttca atgatttcga
     3121 taacaagtac ggttccagta ctgacggcta cgcaacgcaa aaattcagcg ttggctatgc
     3181 ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc caggtcttca gcgagcagaa
     3241 caccagtagc tactcggcag agccgcagtt agacgttaac tactaccaga atgatgttgg
     3301 tccgtttgat acacgtattt acggccaggc agtgcacttt gtaaacacca gagacgacat
     3361 gcctgaagca acccgagttc acctggaacc gaccatcaat ttgccgctct ctaataactg
     3421 gggcagcatc aataccgaag cgaagttgct ggcaactcat tatcagcaaa ctaatcttga
     3481 ctggtataac tccagaaaca caaccaagct ggacgaatcc gttaaccgcg taatgccgca
     3541 attcaaagtt gacggcaaaa tggtctttga acgcgatatg gaaatgctgg ctccgggtta
     3601 tacccaaacg ctggaaccgc gcgcgcagta tttatacgtg ccgtatcgcg atcagagcga
     3661 catctataac tacgactcgt ctctgctgca atctgactac tctggcctgt tccgggaccg
     3721 gacttacggc ggtcttgacc gtattgcctc cgctaaccag gtgacgaccg gtgtcacatc
     3781 tcgcatatat gatgatgctg ccgttgaacg ttttaatatt tccgttggtc aaatctacta
     3841 tttcacggag tctcgcactg gcgatgacaa cataacatgg gagaatgacg acaaaacggg
     3901 ctcactggtg tgggcagccg atacttactg gcgtatctcc gagcgttggg gattgcgtgg
     3961 cgggattcag tacgatacac gtctggataa cgtagcgacc agtaactcca gcattgaata
     4021 ccgtcgggat gaagaccgtc tggtacagct gaattaccgt tacgccagcc cggaatatat
     4081 tcaggctacg ctgcctaagt actattccac agctgagcaa tataagaatg gtatttcgca
     4141 ggtaggtgcc gtcgccagct ggccaattgc cgatcgttgg tcaattgttg gggcctacta
     4201 ctacgacacc aatgcgaaca agcaagccga ctctatgtta ggtgtgcaat acagctcctg
     4261 ctgctatgca attcgcgtcg gttacgagcg gaagctgaac ggttgggata acgataaaca
     4321 acatgcggta tatgacaacg caatcggctt taacatcgaa cttcgcggcc tgagctccaa
     4381 ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt ctgccgtatc aaaacacttt
     4441 gtgatttgtt gatttaccac gtaatccgca gtgcggttaa ttgaaatgga aaaagtatga
     4501 agaactggaa aacgctgctt ctcggtatcg ccatgatcgc gaataccagt ttcgctgccc
     4561 cccaggtagt cgataaagtc gcagccgtcg tcaataacgg cgtcgtgctg gaaagcgacg
     4621 ttgatggatt aatgcagtcg gtaaaactga acgctgctca ggcaaggcag caacttcctg
     4681 atgacgcgac gctgcgccac caaatcatgg aacgtttgat catggatcaa atcatcctgc
     4741 agatggggca gaaaatggga gtaaaaatct ccgatgagca gctggatcag gcgattgcta
     4801 acatcgcgaa acagaacaac atgacgctgg atcagatgcg cagccgtctg gcttacgatg
     4861 gtctgaacta caacacctat cgtaaccaga tccgcaaaga gatgattatc tctgaagtgc
     4921 gtaacaacga ggtgcgtcgt cgcatcacca tcctgccgca ggaagtcgaa tccctggcgc
     4981 agcaggtggg taaccaaaac gacgccagca ctgagctgaa cctgagccac atcctgatcc
     5041 cgctgccgga aaacccgacc tctgatcaag tgaacgaagc ggaaagccag gcgcgtgcca
     5101 ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct ggcgattgct cattctgccg
     5161 accagcaggc actgaacggc ggccagatgg gctggggccg tattcaggag ttgccgggga
     5221 tcttcgccca ggcattaagc accgcgaaga aaggcgacat tgttggccca attcgttccg
     5281 gcgttggctt ccatattctg aaagttaacg acctgcgcgg cgaaagcaaa aatatctcgg
     5341 tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc gccgatcatg actgacgaac
     5401 aggcccgcgt gaaactggaa cagattgctg ccgatatcaa gagtggtaaa acgacttttg
     5461 ctgccgcagc gaaagagttc tctcaggatc caggctctgc taaccagggc ggtgatctcg
     5521 gttgggctac accagatatt ttcgatccgg ccttccgtga cgccctgacc cgcctgaaca
     5581 aaggtcaaat gagtgcaccg gttcactctt cattcggctg gcatttaatc gaactgctgg
     5641 atacccgtaa tgtcgataaa accgacgcgg cacagaaaga tcgtgcgtac cgcatgctga
     5701 tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca ggaacaacgt gccagcgcct
     5761 acgttaaaat cctgagcaac taatggttaa aacccaacgt gttgtgatca ctcccggcga
     5821 gcccgccggg attggcccgg acttagttgt ccagcttgca cagcgtgagt ggccggtcga
     5881 actggttgtt tgtgccgatg ccactctcct taccgaccgg gcagcgatgc tcggtttgcc
     5941 gctcaccctc cgcccttatt cccccacctc ccctgcacaa ccgcaaactg cgggcacatt
     6001 aacgctactt cctgtcgcgc tacgtgaatc tgtcactgcg gggcagttag cggttgaaaa
     6061 tgggcattat gtggtggaga cgctggcgcg agcgtgtgat ggctgtctga acggtgaatt
     6121 tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt aacgacgcag gcattccgtt
     6181 taccggtcat accgagtttt tcgaagagcg ttcgcaggcg aaaaaagtgg tgatgatgct
     6241 ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat ttaccgctgc gcgatatcgc
     6301 agatgctatc acccctgcgc ttttgcacga agtgattgct attttgcatc acgatttgcg
     6361 gaccaaattt ggtattgccg aaccgcgcat tctggtctgc gggctgaatc cgcacgcggg
     6421 cgaaggcggt catatgggta cggaagagat agacaccatt attccggtgc tcgacaagct
     6481 gcgggcgcag gggatgaaac tcaacgggcc gctgcctgcc gataccctgt ttcagccgaa
     6541 atatctcgat aacgccgacg ccgtgctggc gatgtaccac gatcagggtc ttcccgtgct
     6601 aaaataccag ggcttcgggc gcggtgtgaa cattacgctg ggcctgccct ttattcgcac
     6661 atcagtggac cacggcaccg cgcttgaatt ggcgggacgt ggcgaagccg atgtcggcag
     6721 ttttattacg gcgcttaatc tcgccatcaa aatgattgtt aacacccaat gaataatcga
     6781 gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa actttctcaa cgatcagttc
     6841 gtgatcgaca gcattgtttc tgccattaac ccgcagaagg gccaggcgat ggtcgaaatc
     6901 ggtcccggtc tggcggcatt gaccgaaccg gtcggcgaac gtctggacca gctgacggtc
     6961 atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc atccattctt aggcccgaaa
     7021 ctgacgattt atcagcagga tgcgatgacc tttaactttg gcgaactggc cgagaaaatg
     7081 ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca tctccacgcc gttgatgttc
     7141 catctgttta gctatactga tgccattgcc gacatgcact ttatgttgca aaaagaggtg
     7201 gtgaatcgtc tggttgcagg accgaacagc aaggcgtatg gtcgattaag cgtcatggcg
     7261 caatactatt gcaacgtgat cccagtgctg gaagtaccgc cgtcagcctt tacaccacca
     7321 cccaaagtgg attccgccgt cgtgcgcctg gttcctcatg caacgatgcc tcacccggtt
     7381 aaagatgttc gcgtgttgag ccgcatcacc accgaagcct ttaaccagcg tcgtaaaacc
     7441 attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt taacaggaat ggggatcgac
     7501 ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt gccagatggc gaactatctg
     7561 gcggagaacg cgcctttgca ggagagttaa cgatgatcaa ttcgccccga gtgtgtattc
     7621 aggttcaaag cgtctacatt gaggcacaat cttcacctga taatgaacgt tacgtttttg
     7681 cttataccgt aaccatacgc aatctggggc gagcgccagt gcagttgttg gggcgttact
     7741 ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca gggcgaagga gtggttggcg
     7801 tccagccact tatcgcgcct ggcgaagagt accagtacac cagcggtgca atcattgaaa
     7861 ccccgctggg caccatgcag ggtcactacg aaatgatcga tgaaaatggc gtccctttca
     7921 gcatcgacat tcccgtattc cgactcgccg ttcccacact cattcattaa aagaatatgg
     7981 cgacatacct tattggcgac gttcatggtt gttacgatga actgatcgca ttgctgcata
     8041 aagtagaatt tacccctggg aaagataccc tctggctgac gggcgatctg gtcgcgcgcg
     8101 gcccgggttc gctggatgtt ttgcgctatg tgaaatcctt aggcgacagc gtacgtctgg
     8161 tgctgggcaa tcacgatctg catctgctgg cggtatttgc cgggataagc cgcaataaac
     8221 cgaaagatcg cctgacaccg ctgctggaag cgccggatgc cgacgagctg cttaactggc
     8281 tgcgtcgcca gcctctgctg caaatcgacg aagagaaaaa gctggtgatg gcccacgccg
     8341 ggatcacgcc acagtgggat ctgcagaccg ccaaagagtg cgcgcgcgat gtagaagcgg
     8401 tgctgtcgag tgactcctat cccttctttc ttgatgccat gtacggcgat atgccaaata
     8461 actggtcacc ggaattgcgg gggctaggaa gactgcggtt tatcaccaac gcctttaccc
     8521 ggatgcgttt ttgcttcccg aacgggcaac tggatatgta cagcaaagaa tcgccagaag
     8581 aagcccctgc cccactgaaa ccgtggtttg cgattcctgg ccctgtcgct gaagaataca
     8641 gcatcgcctt tggtcactgg gcatcgctgg aaggcaaagg tacgccggaa ggtatttacg
     8701 cgctggatac tggctgctgc tggggtggta cattaacctg cctgcgctgg gaagataaac
     8761 agtattttgt ccagccgtcg aaccggcata aggatatggg cgaaggagag gcggtcgcgt
     8821 cttaaatccc gtcgagcata aaatctaatg cccctttaat caacgttcgg tgcgcgctgg
     8881 catcgcaaaa tttcgttccc tgcgcgttaa ctggaacagt tgcagtctca tgcgtcatga
     8941 gccgtctacc agtaaaagga ttgtgttaag ccgttctgga gggcgaatac ggataaatcg
     9001 ttcaataggt gttaatggaa taacaatacg gcgattaatc tcaccaataa tgtcgctggt
     9061 aacatcgata acaaagggaa acgcggcgtt cctgccgcga ctgcggtata ccgtaaattg
     9121 catcgctaga agctccggta ctcatcagag aaacatccgg tttcatcagc caattgattt
     9181 aacgcagcga tagcttctgc gttctcttca cgccaacgtg ttgctgcatg ttttttaagt
     9241 tcagcattga gcgcaaccgt aagggtggcg ctcatattta agcctgcatc acgagcgcga
     9301 ttgactaact cacggtcgac ggtgacggtc acactttgtg tggtacgttt tgcagtcatg
     9361 atatcgtgct cctgtacaat ttatacgtct agtaaacacg gatatctgca cgtatcagtg
     9421 atgccggaat tctaatatac aaacttaccg ccgctccaga atctcaaagc aatagctatg
     9481 agagttctgc gcatcagcgt cgtggaattc gctgaatacc gattcccagt catccggctc
     9541 gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt
     9601 ttgcgctttg ggcaagaact gctcataaac gcgaccaccg ccaatcacca tgatttctgg
     9661 tacgtcacca cacgccgcga tggcttcatc aaccgatttc acccacgtaa cgcgatcgtc
     9721 agtacccggt tgactgctga ggataatatt tttgcgtcct ggcagcggac gaccgatgga
     9781 ttcccaggta tggcggccca taattacggg tttatttaaa gtgttgcgtt taaaccaggc
     9841 gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc
     9901 taacgccgca atcagactga tcattgagat ttcccgataa aaaaaattgt cgccactata
     9961 cgtaaagcgt aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa ttttatattc
    10021 tgctggcgag tccacgctct ctccctggac tcgccgcatt acaatgaaac aaaaacaaac
    10081 agttagctgt aaagtgtgat ttacgtcact ctttattagg atgagggttt cgtttccggt
    10141 tcatccgcca tgttgccggt atgtttacct tcttcggttc cctgccagcc atgtcgttga
    10201 attaatgaca gatgttcgcg gtcctcggta atgatctcac ttaacatcgc gctggtgcgt
    10261 ttatagaccg ccgcgcgggc tttggtgtcg ttctcaacca ttgccatctc ttccaccatc
    10321 tgaatattaa agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct
    10381 aaactttcca gtgccagacg cccggttttc agcgcacctt cgaaggtttc acgctccggc
    10441 ttttcaacgc ctgcctgacg caaacgaatg tagtggtcga catcgcgggc gcgggcaata
    10501 atctgcaaat gcgggaaatg ttctttcacc atctccgtca gttgcaggtt ggtttgcgga
    10561 tcgtcgatgg cgttaatcag cacttccgct ttcgccgctc ccgcagattc cagtaaatcc
    10621 atccgcgtgg catcgccgta aaacactttc ataccaaatt tacgcaaggt ttcgatatgg
    10681 tccggatcgt gatcgagcac caccattttc accccgctgg agagcagtaa acgtccggtg
    10741 atctgcccaa aacgaccgaa tccggcgatg attacccgcg gctgttcttc gtcgatctca
    10801 tcggcttcac gcgcctcttc actagaagat tgctcaaggc gattgaggat caccagcaga
    10861 atcggcgttg ccgccatcga cagcgccacc gccagggtca gcgatttcgc ccactccggc
    10921 tccagcacat tcgccatctg cgccgcacca aataccacaa aggcaaactc actaccctgc
    10981 cctaacaaca ccgcaaacca gcgacgctgt ttatttggca cctgcaacgg tcgggcaatc
    11041 agccacagca tggcgatttt gatgatgagg aaaccgagca gcagaatgac aatacgcaat
    11101 gggttttcaa tcagcgtgcc aaagtctatc gacatgccaa cgccaatgaa aaacagcccc
    11161 agcagcaaac ctttaaaggg ttcgatatcg ctttccagcg catgacgata ttccgagctt
    11221 gccagcagta cgcccgccag aaacgcgccc atcgccatcg acaagccaac ctcttccagc
    11281 agcaaaccaa agccaaacac gaggaataac gccacggcac taaacacttc ccgcaagcca
    11341 gagcgggcga caaagcgcag cgccggacgc gtgacatagc gccccagcag taccaccagc
    11401 accagcgcac ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca
    11461 ctgctcgccg ccagtagcgg gatcatcgcc accagcggga tcgccgcgat atcctggaac
    11521 agcagcaccg caaaggcact gcgacccatt tgcgtcacca tcagattgcg ttcattcatc
    11581 gcctgcatgg caatcgccgt agaggagagc gccagcgtca tgccgatcaa ttccgcgacc
    11641 tgccagcgca gcccaagcaa catacagaac agccccagca ggccgccgca aatcaccatc
    11701 tgcaatgcgc caccaccgaa caccgccgca cgcagcttcc acagcctttg tggatcgagt
    11761 tcgaggccga taataaacag catcagcacc acgccaatct cggcaaagtg cagaatagat
    11821 tcggcatcgg tcaccagtcg cagcccccac gggccaataa tgcagccagc gatcaggtag
    11881 ccaagtaccg atcccagacc aagacgtacc gcaatgggta caatcagcgc cgccgaaccg
    11941 agataaatca gcgcctgaat cagcgtatgg ctatccatga tgtgcctcct gccattccag
    12001 cagacgttgc ttatagtgcc gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt
    12061 gcagtgcatg gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag
    12121 cggctgcgat agcacatcaa agcccggatg cgcaccaatt tcaaaatggc tttccccgcc
    12181 gccggtcgtc accgcccaca gcaaatgttt gccatgcagc gccgtgccgc cgtgaccgta
    12241 agcccagccg tgtgagaaga ctttatcgat ccaaagttta agaagcggag gaatgctgta
    12301 ccactgcatc gggtgctgcc agacgatcag atcggcgcga gacagcgcct cctgctcggc
    12361 ggcaatatcg atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc
    12421 cagcgtcctt gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg gatacggatg
    12481 cgcataaatt ataagaatca taggtagccc gcgtgattac gcctttgttt tcccacaaag
    12541 tgtagtcata tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc
    12601 tgaacaaatt accccattct ctgcccgcgg atggtcatat tgctggtctg cgccagtgac
    12661 atccctcgag tctccggggc aaacgctacg gaaatcaaca agccaaacag cgagataccc
    12721 gcccccatta gcatcgtgtt actgataccg taattgttga taaagatcgg cagtgcccag
    12781 gtcgaaacaa tagtgccaat acgacttaag gacataatca cgcccacggc agaggcgcgg
    12841 atatctgtcg ggaagagttc attaggatag agccactgca aattacctgg cccgccagag
    12901 aaaaaggcat acaccgcaaa ggccatcact accagccaga tccccatatc cgggatcagc
    12961 cccaaaaccg ccagcgccag cgtcatcatg gcaaagctgc caatcaacaa tggacgccgt
    13021 ccggcagtgt ttaaccacag catcggcgga atacagccaa gcataaagaa caggctaatc
    13081 accacgttcc ccagtgccgc gtttttgcca acacccaatc ccaacaaacc aacgatttgc
    13141 gggccaaagg tgtaaatggc gaacattggg atcacctggc aggtccagat ggcggcaaca
    13201 aacagaacaa aaggaaaatg gcggcgatta aacagatcgc gaaaacgggt ttgctgcggc
    13261 tgctcttcat cgaaagccac cggttcgcca aacagtttgc tcatcatctc ttcgcactct
    13321 tttactcgcc ctttgcgtaa caaccagcgg ggagattcag gcagttcgaa tcgaccaatc
    13381 aaaatcaaca aacaagggat cgccgcgcta cccagcatcc agcgccagcc gccttccaca
    13441 tcataaagcc agtagccgac cagatcggcg caggtcgcac cgacatacca catggcggca
    13501 ataaagctga tggaaaacgc ccgctgacgg gtactggaga actcggtgat catcgaagtg
    13561 gcgatgggat aatctgcacc gatgacaata ccgatgagta cccgcatcac caacagttcg
    13621 acgggggatg agacaaacat cgtcgccacc gatatcacgc cgatggcgat gatatcaatg
    13681 aggaacattt tgcgccgtcc gactttatcg gagatataac caaacagcga tgtgccaacg
    13741 aacagcccgg cgagcgttcc cgcgcccagc aagccaatcc agtcagcgtc cagcttcagc
    13801 gccggagtca gttgctccag cgccacgcca atcattacca gtacataacc atccagaaac
    13861 ggaccaccgc ttccccacag caaaatgcgg cggtgaatag aggaaaattt aagatcgtca
    13921 aagtttctgg acggttgcat gggttattcc tgtttttttg caatgagagt cagtacggcg
    13981 ttacgtatca aaccaacatc agccgtaacg gaactccaca ccaaaggtgc cgcgcgggta
    14041 ttcccactgt tccagcgccg accccagccc cagaatgcga caggtgccgc actccagaca
    14101 tccggcgtaa tcgaagcgca cactgccgtc atcctgcttc ttgtacagac ctgcggggca
    14161 cgctttcacc agcagctcca gcgcctgttt atcggcatcg gccttcacaa caatgtgcgg
    14221 atgctcttca tcgacattga atttattgac gcccagtttg acgtccacat tgacgggaga
    14281 agtcataaaa cggtcactcc tttcatgcca tccttgatca gattgatgaa gcccactttc
    14341 ttgccgtggc ggaggatttt cttgcgcatc agttccggcg cactgccgtc aatggtgaac
    14401 aggtcacgcg ccacgcccac cgccagttcc gggtagccgc taaacatgcg tgggttatca
    14461 aggaacgccg gtagtttctg gtacatacgc atatcgcgca gcgggccact ctcaagatgc
    14521 tgacgatatt ccgccagttt ttgcttactg aaatcgtcgc ttttcatcgc tgaaagcacg
    14581 gtttttgctg cggcttcccc ggcggcaatc gccagatcca taccgcgaat ggtaaaaccg
    14641 aggttcatac acattccggc ggcatcaccg gcaatcaata cgccgtcacc aaccaactcc
    14701 ggcagcatgt tgatgcctgc ttccggcact acgtgagcgg aatattccac cagcttgccg
    14761 cctgcgatca gcggtgcaac ggccggatgt tgtttgaaat cttccagcat ttgcggcacc
    14821 gattttttcg cgtcatgcag atgatgcaga ccacaaacca accccagcga cagagtgttt
    14881 tcattggtat aaagaaagcc gccgcccatc aggccattgg tgggtgatcc cgcaaacagg
    14941 caagccgcgc cctgattacc ctgcaattga aaacggtctt caataaccga cttcggtaac
    15001 tcgatcagtt ccttcacgcc aaccgccaca tccgtcggtt tgacgcgttt tgccatcccc
    15061 agcttttcgg caaggatgga attcaccccg tcagcaagga tcaccgtttt cgcttcaatt
    15121 acatcgccat cggcttctac gccgacaact ttgccatcac gctgtacgag gttatcgacg
    15181 cgaatcccgg taattaactg cgcgcccgct tcttcggcct gctccatcaa ccaggcatca
    15241 aatttactgc gcaaaacgga gtaagaacgc tgggatggcg aggtttcgtc accgttgcag
    15301 taatccatag tcatcgctga cttttccgtc ataaacgcga gtttttcatg ggtgatcagg
    15361 cgttctacgg gggcggattc ggcgaaacct ggaataatgt gttccagact gtgggcatag
    15421 agacgcccgc cggtgacgtt cttggcacct gcggaattgc cacgctcgat aactaacact
    15481 tgcgcccctt cgcgggcgag caccagtgcg gcaaccgaac cggcaagccc tgcaccgacg
    15541 atgatggcgt caaagatatc ttcggacata agagctccct gtcagcggca gggccgaaat
    15601 gcagccctgc cagagtggat caacgcgcta aagctgcggt cagagccgga aggatcttca
    15661 cggcgtcgcc aacaatgccg taatccgcgt actggaagat cggcgcattt ttatctttat
    15721 tgatggcgaa aatggtttgc gatgcattag caccaaccat gtgctggatc tgcccggaga
    15781 tccccaccgc caggtacagt tcaggtttca gcatcaggtt ggagataccg acatagcgtt
    15841 cgtgctccat ccatttttcg ttttccgcca ccggacgaga acaggccaac tccgcaccta
    15901 tcgccttaca aagctgttct gccagcgcaa tgttctcttt gctgccaatg ccgcgaccga
    15961 cgctgaccac cagacgggct ttgtcgagat cgacgctgtt gctctggcgc gcctgggttg
    16021 ccgtgcgggt aatcgccaca gccggagcct gccactccac ggtgtgcgtt tcgccagtgc
    16081 gtgacgcgtc tggctgagcc gcatcaaacg tgccgctgct gatggtcagt accgcatacg
    16141 gcgtggcaat gcgttcttcg ccaatcgcca gaccgccgta aaccatgtgt ttcactgtcg
    16201 ctttcccgtc ctgtacgctg acggtgctgg catcgttaga caccgccgct ttaaggcgat
    16261 aacccagttt tgccgccagt aatttgccgc gacgggtatt cggcaacagc accagaccgt
    16321 ctgcgccgtg ctggcgaata gtgtccgcca tgacaccggc gtaatcttcg accatccgat
    16381 cgtccggttt gccgtttaat ttccagacat gattagcgcc tagctggatt gcctgtgcgc
    16441 cgtcggcatc attgaggaca aaggcattga tttgattagc taaagcctgc gcaccgttca
    16501 tcagttccgg cagacgagaa ggggtatcgc tgaataccca gacttgagaa aacgtgttca
    16561 tagcatcccc tgtaattaaa tgactttgcg aagattttca gcaaatgcgg cgatctgttc
    16621 ttcgccgtcg ccttcaatca cgatgcgctg acgctcgcgc tgtttcggcg cggcaacctg
    16681 ttgttctgac caggctgcct ctgcgttaaa accgatatcc gccgccgacc atacctggac
    16741 tggctttttc gccgcgccga gaatggcttt catcgaagga atttgtgggg agttgatatc
    16801 agtggaaaca gcaacaaccg caggcagcgg aatgcttaag gtttctgttt catcttccag
    16861 ttcgcgctca acggtgaggg tatctgccgt cagggagata attttgctga cgccgttaac
    16921 tgccggaata ttgaggattt cgcctaccag caaaccaacc tgctgggcat aaaggtcgga
    16981 agaaccatcg ccacagagga tcaaatcaaa gcctgctttc tgggcggctg cagcaagtac
    17041 gctcgccgtt tgttgcggca gtgcctgctc gaactggtca tcaatcacca caatcagttc
    17101 atccggaccg cgcgatagca catctttacg ccctttggcg ttggtcaggg ctttaccgcc
    17161 cacacttagg gccgtcacct gcgcctctgc tgcctgttgc tttagctggc aagccgcttc
    17221 aatagcgttg agatcgtatt ggcttatttt ggcatcggct ttactgaagt ctaacgaacc
    17281 atcagcatta ttgaccgcaa tatcctgttc atcaggcacg cacttatagc aagtaataat
    17341 cttcattgca tctccagaaa tcatgaagga aacgtcgttc atatcggtgg tgttaattac
    17401 agatatcacg ccctttaatt tggaaataaa actttaatca ccaatattga aaatgtcaca
    17461 cgcagataaa tccaactttc aatattgtta agtccctcac caatattgaa aacacggcg
//
LOCUS       DAOTRK010000103        17043 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.103, whole genome shotgun sequence.
ACCESSION   DAOTRK010000103 DAOTRK010000000
VERSION     DAOTRK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.103"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(10..312)
                     /locus_tag="QIE41_003235"
     CDS             complement(10..312)
                     /locus_tag="QIE41_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532089.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(309..935)
                     /locus_tag="QIE41_003236"
     CDS             complement(309..935)
                     /locus_tag="QIE41_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HDS3532090.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            complement(1131..2402)
                     /locus_tag="QIE41_003237"
     CDS             complement(1131..2402)
                     /locus_tag="QIE41_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDS3532091.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIKMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPDFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGTVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(2402..2839)
                     /gene="umuD"
                     /locus_tag="QIE41_003238"
     CDS             complement(2402..2839)
                     /gene="umuD"
                     /locus_tag="QIE41_003238"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDS3532092.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(2836..3084)
                     /locus_tag="QIE41_003239"
     CDS             complement(2836..3084)
                     /locus_tag="QIE41_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDS3532093.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            3502..4404
                     /locus_tag="QIE41_003240"
     CDS             3502..4404
                     /locus_tag="QIE41_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDS3532094.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(4499..4834)
                     /locus_tag="QIE41_003241"
     CDS             complement(4499..4834)
                     /locus_tag="QIE41_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532095.1"
                     /translation="MRTITTREQLLVNGKVRERIATHIVTGAHGYETLCTSGYNLQYN
                     KERVLIENCEKVADGELPVTCHTCFSIWQDVHRFKPGDFDTESGKGNFTDTELTKITI
                     GQEKTPNAC"
     gene            5172..6152
                     /locus_tag="QIE41_003242"
     CDS             5172..6152
                     /locus_tag="QIE41_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HDS3532096.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ATVPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVD
                     IVVTLPLGEYLDENDQPNLANIERKKNNVRRTVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQGRDDNEFLTRYIPNAEQRERILTVLRDREKKLHYRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            6155..6577
                     /locus_tag="QIE41_003243"
     CDS             6155..6577
                     /locus_tag="QIE41_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDS3532097.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNN
                     AKAILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            6581..6892
                     /locus_tag="QIE41_003244"
     CDS             6581..6892
                     /locus_tag="QIE41_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532098.1"
                     /translation="MNSVKGRCCVLFSFPTKDVAGLLFLLRRLTRRGRNAACGQYPAS
                     GRARDGRFYRSRLASVAVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            6968..7651
                     /locus_tag="QIE41_003245"
     CDS             6968..7651
                     /locus_tag="QIE41_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDS3532099.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVQGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            7652..7873
                     /locus_tag="QIE41_003246"
     CDS             7652..7873
                     /locus_tag="QIE41_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532100.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            7887..8321
                     /locus_tag="QIE41_003247"
     CDS             7887..8321
                     /locus_tag="QIE41_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDS3532101.1"
                     /translation="MYGTCETLCRELAAQYPGDAPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVVNQARTLLKNNTREND"
     gene            8366..9136
                     /locus_tag="QIE41_003248"
     CDS             8366..9136
                     /locus_tag="QIE41_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532102.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRSARFSSAEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRDVLREVVREARAEIRSMADALAKIRG"
     gene            9672..9974
                     /locus_tag="QIE41_003249"
     CDS             9672..9974
                     /locus_tag="QIE41_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDS3532103.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            10021..10443
                     /locus_tag="QIE41_003250"
     CDS             10021..10443
                     /locus_tag="QIE41_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDS3532104.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            10440..10631
                     /locus_tag="QIE41_003251"
     CDS             10440..10631
                     /locus_tag="QIE41_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532105.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            10769..11032
                     /locus_tag="QIE41_003252"
     CDS             10769..11032
                     /locus_tag="QIE41_003252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532106.1"
                     /translation="MPGTDVIFRDTVTVYGCLLLRPLRLPAAWRHPVVRPDSDRQLLA
                     HPPVSAEPAGAVSGCRYGPMRENPGLQRPLRSRRPCRAAPVAP"
     gene            11416..11943
                     /locus_tag="QIE41_003253"
     CDS             11416..11943
                     /locus_tag="QIE41_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDS3532107.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNVQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            12001..12234
                     /locus_tag="QIE41_003254"
     CDS             12001..12234
                     /locus_tag="QIE41_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDS3532108.1"
                     /translation="MSEYFRILQRLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            12293..14251
                     /locus_tag="QIE41_003255"
     CDS             12293..14251
                     /locus_tag="QIE41_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDS3532109.1"
                     /translation="MPVTKCEPETTRKASRKYAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYLPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLDSAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            14304..14738
                     /gene="psiB"
                     /locus_tag="QIE41_003256"
     CDS             14304..14738
                     /gene="psiB"
                     /locus_tag="QIE41_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDS3532110.1"
                     /translation="MKTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMVEGGQA"
     gene            14735..15454
                     /locus_tag="QIE41_003257"
     CDS             14735..15454
                     /locus_tag="QIE41_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDS3532111.1"
                     /translation="MSVRSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            15451..16047
                     /locus_tag="QIE41_003258"
     CDS             15451..16047
                     /locus_tag="QIE41_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532112.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            16509..17009
                     /locus_tag="QIE41_003259"
     CDS             16509..17009
                     /locus_tag="QIE41_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDS3532113.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         3704 a         4419 c         4876 g         4044 t
ORIGIN      
        1 agtaaggtgt tatctttgtt ttacttctgc tatcaatcgt gatacctctg tttttgatag
       61 tgttttaagt gcttctaccg ccgccctgac aacatcagaa cgattcactc tgcctttacc
      121 tgaaaccatc tcagccgtta taagggcatc aatgtctttt aggttttctt caaaaaaaga
      181 aatactcact ggttttgctt tgcttacaac cgatgattta tctgatgact ttttggtatt
      241 atcaaggcca gccgtgccac cgttaataaa tgctgcctcg tcacgaggtg aagtgtttgc
      301 tctatttctc acagaataac ctctttcact aacgcttcga tttcttgctg ggctttatca
      361 ttttgcgttt caaaaattgt cattgattcg gcccaagcat ctcgatgtgc tttacgatca
      421 catacccggg agtttgccag atgcatttca gggtaatcct taagtacttc agctgcctca
      481 ttggcttcat taacaaacat atttgtgggc gtcatgttta aaacgagata tccccgaaca
      541 tcctcattaa aatcttttgc tgctgtaaaa acggagcatg tatgaggcac aacatccagg
      601 tccatctgag atgggcgcaa aggagaaatg aatacatctg cggccatcaa tccactacgc
      661 atttctgcgc tatcacggcc agcacagtct gcaataacaa aatcatagga tttttcgtgt
      721 tcctttaaca tggctttgat gttacctgtc gcgcctgtaa ccggaatatg agggagatct
      781 tcagggcgat tgttgtacca ggttaatact gatttctggt catctgcgtc gacaataatc
      841 actctattgc ctagtgacat cagatacgca gctatagata ccgccagtgt acttttacct
      901 accccaccct tttgactacc taatactatt atcattatag cctcatatta agctgttaag
      961 ttaacaactt aatattgcca tctgaacgcg aaccatgcaa ttatttttgt tatgttaaca
     1021 acttaacaag ctgttaagta tttgatttat agattaatta agataacaag ataacaagat
     1081 aacaagataa caagataaca agataacaat attatgggtg gattacatat tcataactgg
     1141 gctactggca aatctgtcca tctggtggta tacgcaggag aaagcatttc ccgtttcatt
     1201 tgccactcag gggcggttcc tcgccccgca aaccacactg tcccttttcc cgactggttc
     1261 agttcatcga gtgttttcat taacttttca ctgtttttac ggggctggat ttcatcaaat
     1321 aatcccggct gtgctatacc cgatggtgtg aaatcagcca gcatgactcc tgccttcata
     1381 tagcggtacc cttcacgcca gacatggttt aaggctctgc atgcggcggc aataatgtcc
     1441 cggctgtcct gtgtgggcaa tggaagcttt tccacagcgg cattgctgta acagggttct
     1501 tttactgcaa agggggatgt ccgtacaaat gtcgtcacct gccggcaata ctgacgctcc
     1561 ccacgtagtt tctctgcggc ccgctctgca tactgaacaa cagcctggtg catggcatct
     1621 ttgtctgtga ttcgttcacc aaaactgcga ctacagacaa tctgctgttt tgccggtggt
     1681 gcttcttcca gggatatgca ggactcgccg ttgagttcgc gtaccgtacg ctcaagaatg
     1741 acgctgaagt ttttccggat gaatgccgtg ttagcctgcg ccagctgcag tgctgtgtta
     1801 atacccagcg cattcagctt ttccgtcagt ctgcgtccta ctccccagac ctcaccaact
     1861 ggctgcagcc ccagtagctt caaggtccga ttacggtttt ctgccgtcag cgcgaccact
     1921 ccggaaaact gtggccattg ctttgttgcc cactgtgcac ttttagccag cgtttttgta
     1981 ggcgcaatgc ccacccccat ggtgagtcct gtccagctct ttacctgttc cctgagctga
     2041 tgaccaaaaa aatccggaga gatgcaatga tttatccccc gcaaatcaat aaacatttca
     2101 tcaattgagt agggctcaac tgcgggagaa agcgactcca gaacagccat gacccgttgg
     2161 ctcatgctgt ggtacagcgc ataattgctg gaaaatacat gtattttctt ctccaggcgc
     2221 atctgtctca cctgaaacca gggctgcccc attttgatgc caagggcttt tgcctccggg
     2281 ctgcgcgcga tcacacagcc atcgttattg ctgagtacga tgaccggttc gttgcgaagg
     2341 tccgggcgga aaactttttc acatgaggcg tagaaactgt tgatatcagc cagtgcaaac
     2401 atcagcgtaa ctcccgcgtc ctgtgtatca cgtgagtgac aacaccaaaa atacagatgt
     2461 tttccggata cagtgtgcgg aactccgggc tgtctgaaac cggctccagt gccgggcgtg
     2521 ggcgcaacag cagtcgtttg acggtgaact caccgtcgat ctcagcgata acgatgtccc
     2581 cgtgttgtgg tttttcggcc ctgtccacta ccagcagatc accattctgc acgccagcct
     2641 ggttcatcga ttcaccgctg gcgcgcagaa agaaggtggc tgcaggtctg ctgatgcaat
     2701 agctgttcag atccagttcc tgctcagcat aatcagtggc aggcgaagga aaaccggcct
     2761 ggcaacgatc ggcaaacaac ggtcgcacgt aactgtcatc gcctgaaggg tcagccggac
     2821 ggtgataaac ggtactcatg ctgcaggcag ccagctgtcg tcctgccaga tttcttccag
     2881 cagagacagg atacgttctt tatccttgtc gttgcgggct cctgtgacag atacagacgg
     2941 ggcgctgccc tggtctatac gcagccagat ttccgggtag tgtggtttca gacgttgttc
     3001 gatttcattc tgtagcgcct gaagtgtgcc tgatttgagg ttttttgtgc tctggcggtc
     3061 gaaaagaatt tcaatgcgga tcatgttctc atctcccttt tctattgctg tatgcatata
     3121 cagtattcta ataactgaat ccacacacag tcaaatgtca ttattgtgac taaaaaacgc
     3181 tcaattctct gggagtgtgg ctgtatgcct gctgtggact gctgtgtcca gcttatccac
     3241 agcattttgc gcacggttct gtgaataaaa tacctggtta cccaggccgt gccggcacgt
     3301 taaccgggct gcatccgatg caagtgtgtc gctgtcgccg gcctcctcac ccggtcacgt
     3361 ttcgtcgttc ctcctccacg cgctgcggct tcggggccgc acctgcattc gtatgcggtc
     3421 gcccggttac aggtgcggca cggcctgatg gaggccgcat gtgagaggag aattcccatg
     3481 ccaaactggt gctcgaatcg tatgtatttt tctggtgaac cagcacagat cgctgagatt
     3541 aaacgactgg ccagcggtgc agtcacaccg ctttatcgcc gcgccacaaa tgaaggtatt
     3601 cagctgtttc tggccggaag tgccggactt ctgcagatca ctgaaaatat ccgctctgag
     3661 cagtgccccg gagtgacggc tgccggacgt ggtgctgtat caccggaaaa tatcgcgttc
     3721 acccgctggc tgacacacct gcagaacggt gtactgctgg atgagcaaaa ctgcctgatg
     3781 ctgcatgaac tctggctgca gagcggtact ggacagcgtc gctgggaaga gttaccggat
     3841 gatgtcaggg aaactatcac tgtgcatttc accgcaaaaa gaggtgactg gtgtgacatc
     3901 tggggtaacg aggatgtatc ggtgtggtgg aaccgtctgt gtgacaacgt actgccggaa
     3961 aaaaccatgc cgtttgacct gctgacggtt ttgccgaccc gcctggatat tgaagtgaat
     4021 ggctttaacg gcggtgttct gaacggtgtt ccttctgcat atcactggta tacagaacgg
     4081 tatggcgtga agtggccttg tgggtatgac ctgaatatca gtagtcaggg agacaacttc
     4141 attcaggttg atttcgacac gccgtggtgt cagccggaaa gcgacgttat tgcagaatta
     4201 agccgccgtt tcagctgcac gctggagcac tggtatgccg aacagggctg tgatttctgt
     4261 ggctggcagt tgtatgagcg cggagagctt gttgatgtgc tgtgggggga gctggaatgg
     4321 tcttccccga cagatgatga tgagctgccg gaagtcaccg gacctgcgtg gatagtcgac
     4381 aaggtggcgc attacggtgg atgaagtatg acggagacgg gcgggcaacc gcccgtttct
     4441 tttccggtaa aggatgtcgc catgctcctc tttttactgc gccggctgac gcggcacatc
     4501 aacacgcatt cggcgttttt tcttgtccaa ttgtgatttt cgtgagctcc gtatcagtga
     4561 aatttccctt tcctgattct gtgtcaaagt caccaggttt gaaccggtgt acatcctgcc
     4621 atatagagaa acaagtgtgg caggtgacag gcagttctcc atcagccacc ttttcgcagt
     4681 tttcaatcag tacccgttcc ttattgtatt gtaagttata accacttgtg caaagcgttt
     4741 catacccgtg agcgcctgtc acgatatgtg tggcaatccg ttccctgact tttccgttca
     4801 ctaataactg ttcacgtgtg gtgatcgttc gcattctctt cctcatcagc tttcctgtag
     4861 aagcctcctg actataaggc tccggatatc ttacccgctc attttggctg gaggtagtgg
     4921 tggcgtcaga ggatgaatta ctgacattgt atatgaagct ttgagttttt tctggatttc
     4981 atcagtgttt atctttattt gttcttgttt gttctctttt gtgctgttgg agtcggtttt
     5041 tgtctgtgtt tttctttatt tttccatttg tggccgatat caggtcttta ccgatacgtt
     5101 gttttaggtg ataatcgttt ttgatctcgt atttttctaa aaaaatccat tttatttatg
     5161 tgaggttcta gatgcgtatc tttgtagacg acggctctac caacatcaaa ctggcctggg
     5221 ttgaagacgg tgcagtgaaa acgttaatta gtccgaatag ttttaagccg gaatggtcta
     5281 tgacgctgca gggactgact gatgccactg tacccgcttc cgcaaactat gagattgatg
     5341 gagaaaaata cagctttgat caattgagtc cggatgcagt gcgcaccaca gaaacgcgtt
     5401 atcagtacag cgacgttaat gtggttgcta ttcatcatgc gttgatgcag tcaggtattg
     5461 ctccacaacc tgttgatatt gttgtgactt tacctttagg tgagtatctg gatgagaacg
     5521 atcagccaaa tctggctaat atcgaacgta agaaaaataa tgtccggcga actgtaacag
     5581 ttcaggggcg tgaaaacttt accattcgta aagtcagtgt cttgcctgag tccgtgcctg
     5641 ccggttttga cgtgctgaaa gatctgaatg atctggagtc gctgttgatt gttgatatcg
     5701 gtggaacgac ccttgatatt gcacatgtcc gcagcaaaat gtcaggtttc acaaaaacct
     5761 attgtgaccc caagactggc gtctccatca ttactgaagc agttaagcat gcgatggatg
     5821 tgaatgtgag tacacgaacc agttcttatt ttgcagatcg tctaatccag ggccgggatg
     5881 acaatgaatt cctgacccgt tatatcccga atgcagaaca gcgtgagcgg atcctgacag
     5941 tattacgtga ccgggaaaaa aaactgcatt atcgtgtggc ggattcagta ggacgctttg
     6001 ctggttttac tcatgttatg gttgttggcg gtggtgccag ccttgtggct ggggcagtaa
     6061 aaaaagcgac aggagtgggt gataaccgtt tctttgtcag tgacaatcct cagttcgatc
     6121 tggtgttggg aatgatggca atgaagggat gagtatggct gataatcgta agtgctcttt
     6181 ttatatttat ccggaacgaa atgcagctga tcgggtggct gacaggtttc ttgaaaaatt
     6241 gcctcagaaa gaacgtggtc gggcaatgcg ggccatgatg ttgtgtggtg ctgcattgat
     6301 gaagcaggat gaacgattgc cgttcctgat agctgaattc ctgacagaca gcacgtctat
     6361 gcaggatatt caacgaatta tcagtagtac gctaccacaa caggaaaatg gtgaagtcgt
     6421 tcgtttgctg gaggcatttc tgcagtctgc tggtaataat gcaaaagcga tcctgcctgc
     6481 ggttgattct gctacgcaag aaatatctgc tccagtcgat cagaatctcc tggaaacaag
     6541 aaacaacatt aagaacttgt ttccggatga tgaataagaa ctgaactctg ttaaaggacg
     6601 ctgttgcgtc cttttttctt ttccgacaaa ggatgtcgcc gggctcctct ttttactgcg
     6661 ccggctgacg cggcgcggca ggaacgctgc ctgtggtcag tatcccgcgt ccggccgggc
     6721 acgggacggg cgcttttacc gctcccggct ggcgtcggtg gcagtgtacg ccagcccgtc
     6781 gcccttttct gatgaacgcc cttcaagccg ctttcgcggc atagccttgc cgtcaaaaag
     6841 acgacggctg cggtattcca cggtcggcct gacccgttac cggacgcggt gaacagccca
     6901 caggcagcgg ggaacgggca ccacaggggt gccctcccgg tgccctctgt aagagaagga
     6961 gttttgtatg tcccgttttg tcctcggtaa ctgcatcgat gttatggccc gtatccctga
     7021 taacgccatt gatttcatcc tcaccgaccc gccgtacctc gtcggttttc gtgaccgtca
     7081 ggggcgtacc attgccggcg ataaaaccga cgaatggttg caaccggcct gtaatgagat
     7141 gtaccgcgta ctcaaaaaag acgcgttaat ggtgagcttc tatggctgga atcgcgtcga
     7201 tcgctttatg tctgcctgga aaaatgcggg attcagcgtt gttggtcacc tggtcttcac
     7261 caaaaactac acatcgaagg ccgcatatgt gggctatcgc cacgaatgcg cctacatcct
     7321 ggcaaaagga cgtccggcgc tgcctcagaa gccattgccc gacgtgcagg gctggaaata
     7381 ttcaggcaat cgtcatcacc cgactgaaaa gcccgttacc agcctgcaac cgctcattga
     7441 gagcttcaca cacccgaacg cgattgtgct ggacccgttt gcaggcagcg gctcaacctg
     7501 tgtggccgcc ctccagtccg gacgccggta tatcggtatc gagctgcttg aacagtatca
     7561 ccgtgccgga cagcaacgac tggccgccgt gcaacgggcc atgcagcagg gggccgcgaa
     7621 tgataactgg tttgaaccgg aggctgcgta aatgaactat gccggacacg aaaaacttcg
     7681 cgccgacgtg gcggaggtgg ccaacaccat gtgtgacctt cgggccaggc tgaacgatat
     7741 ggagcatcgc tgtcgctttg attccgatgt gcttgttgaa cgcctgacac gccagacgct
     7801 gtatcgcgcc aatcgcctgt ttatggaggc gtataccgaa attctggaac tggattcctg
     7861 cttcaaagat tgagaaggag acgagaatgt acggaacatg cgaaacgctc tgccgggagc
     7921 tggcagcgca gtatccggga gacgcgccgc tgatgctggt tgtctggtcc ccggaagaga
     7981 tccaggccct cgctgacgga atggatattt ccctgtccga tcatgaaatc aggaccgtcc
     8041 tggcgcgcct ggaggacatc ccggaagacc agcggattga gtccggtatt tcttccgccg
     8101 cggcgatgga gattatcagc aacgtgagcg aaaaccgcca ggtgaccgtt ccggctgaac
     8161 tgctggcgtc cctgattcag accgctgaac aggcattgtg gaaacgtgaa tgggccgccc
     8221 gggatcatgg cctcgccgtc ccggaatgcg tcacccgccg tcaggcggtg gttaatcagg
     8281 cccgcaccct gctgaaaaac aacacacgcg aaaacgactg atgttatcgc cgcctgcggg
     8341 cggcgattca gggagaccat gaatcatgaa cgaaacgctg aatgcactga tttgtcgcca
     8401 cgcccgtaac ctgctgctgg cgcagggctg gccggaagag acggatgttg accagcgaaa
     8461 tccgaaccat ccgggctgga tcagcattta cgtgcggctg gatgcgcccc ggctggcgac
     8521 gttactcgtt aaccgccacg atggcgtgct gccgccgcac ctggcttccg ccattcagaa
     8581 actgaccgga accggggcgg aactggtact gtccggcagt cagtggcagt cgctgccggt
     8641 acttccggca gacggaacgc aggtgtcttt cccgtatgcc ggagaatggc tgaccgaaga
     8701 cgaaatcagg gcagttcttg atgcggtgcg agatgcagta tgcagtgtca gttgccgggt
     8761 ggcagaagat gcgcggcgta tcagggcggc gctgaccaca accggtcaga cgttgctgac
     8821 ccgccagacg cgccgctttc gcttggttgt gaaggaaagc gatcacccct gctggctcga
     8881 tgaagatgac gaaaacctgc ccgtggtgct cgatgccatc ctgaaccgga gcgctcgttt
     8941 ttcgtcggcg gaaatgtacc tggtcagtga gtgtgtggag catatcctgt ccagtgggct
     9001 ggcctgcgat gtgctgcgta taccggatga accgccccgc cgctggtttg accgtgatgt
     9061 tctgcgggag gtggtccggg aggcccgggc cgaaatccgc agcatggcgg atgccctcgc
     9121 aaaaatccgg ggctgatgat ggcggggaaa atccccgcct tttcctgcca gtccccggac
     9181 agagcacaaa ggaagtcgcc gccggctccg ctttacccgg ccatgcggtg catggccttg
     9241 tgggttttca gttatgtggc cgccgtgtcg tgtgcgggct gtgccgtgcc tccatcttag
     9301 ccgggctggc ggggatgcaa gggtaaaatg aacgcggcgg tcacccggtc cctccttccc
     9361 gtctgccgtg attttccggt cgttcacccg ctcagatttc gcggtctcac cctccaactc
     9421 ccgccagccg tgtgcgtcag gctgcggctt cccttgcgcc ctgtgcatcc ccgccttatc
     9481 gcccggcttt tatggaggca cggcaccgcc cggtgtcgcg gaatatgtga acaacggagg
     9541 aaaacatcat gcaatatgcg aaacctgtca ctctgaatgt tgaagaatgc gaccgcctct
     9601 tctttctgcc ttacctgttt ggccaggatt ttctgtatgt cgaggcgtct gtgtacgcgc
     9661 tggcgaaaaa aatgatgccg gaatacgaag gcggattctg gcacttcatc cgcctgccgg
     9721 acggtggcgg ctacatgatg ccggatggcg accgtttcca catggtgaac ggtgcaaact
     9781 ggtttgaccg taccgtgagt gctgatgccg caggcatcat ccttacctcc cttgtgatta
     9841 accgccagtt gtggctgtac cacgacagcg gggatgcagg actgacccag ctttaccgga
     9901 tgcgcgatgc gcagttgtgg cgtcacatcg aatttcaccc cgaatgcaac gcgatttacg
     9961 cggcgctgga ctgattaacg gacggggcgg caatgccgcc cctgtaaagg agacgagaac
    10021 atgtactgta ctgttaaaga aatcatccgc gatgtactgg atacagacgt gccggacagt
    10081 gaatgcgttt ttgccgtggt gctgacccgt ggggatgtgc gtcacatagc ccaggactgg
    10141 agtctgacag acgatgagct ggaaaccgtc atgcagcggc tggacgatgc ctttgaatat
    10201 ggtgcggatg tcagcattgt tcacgacgtt gttcgtgaac tgatggaaga aaagcgcgcc
    10261 agccgtcatg tgacagtccc ggcggtgatg ctggaaaaag tgatggcgct ggcaggcagt
    10321 gaaatgaagc gcctgtatgc cgtcgggagt gagaacgggg gcgacggtga cgcattcgtc
    10381 agggaggaac gcgaagcaat ggacgttgtg ttacaggcgc tggacgggga gacgatgtca
    10441 tgaatatcag cacagaaacc cgcgaaattc tgcgtaatta cagggccgtg attaatgcgc
    10501 ggcgtcgtga aatggggcag aaaccgctca ccaccgcgca gattgttgat gaaatctgcg
    10561 attttgtggc gaatcagcag gcggttttcc tgggcggtca ctacatctta cagggcagca
    10621 gaaacaggtg atacaggggc aggcggtgga ggccgcctgt cagcccgcgc gaaaacccct
    10681 ggcaggctga cgcctgccgg aaacgcgggg tgcagcgcct gcggcgctgc cggcccggct
    10741 ttccggtcag aaggtagtgt atggctgtat gccaggaaca gatgtgatat tccgtgacac
    10801 tgtcaccgtt tatggttgtc ttttattgcg cccacttcgg ctcccggctg cctggcggca
    10861 tccggtcgtc aggcccgact ccgacaggca gctgctggcg cacccgccag tctccgccga
    10921 acccgctggc gcggtttcgg gctgccgcta cggtccgatg cgtgaaaacc cgggcttgca
    10981 gcgaccgtta cggtcgcgcc gcccctgtcg cgccgcccct gtcgcgccgt gacctgcgct
    11041 ccctgcacac ctgacggtgt gtgaggaaac tgcgcctgat ggcgcagcag tcctccctcc
    11101 tgccgccacc cctgcggcct gtttcccgct gtggactttc cggggggcag acgtaacccc
    11161 ctcccgttcc atctgtccag ggtgagatgc accggccaca aaatttttag ccatgaaccc
    11221 ggcaaaaaat tttgcgtccg cccccggaca gctgtcccgt gaggcggtaa acgggccccc
    11281 atcggaaagg tccactgcgg ggagaaaccc cgaagaagtg gacggcagga ggagctttcg
    11341 ctcctctgaa ggggcacatg aacgtcccgt cgccggtatc cggtttatct cacatctttc
    11401 agtcaggagg ttcttatgag tgcacgtggt atcaacaagg tcatcctcgt cgggcgtctg
    11461 ggcaatgatc cggaagtccg ttacatcccc aacgggggcg cagtggcaaa cctgcaggtg
    11521 gccacatcag aaagctggcg tgacaaacag acgggggaga tgcgggagca gacggaatgg
    11581 caccgcgtgg tgctgttcgg caagctcgcg gaagtggcag gtgaatatct gcgcaagggt
    11641 gcgcaggtct acatcgaagg tcagcttcgc acccgtagct gggaagataa cggtatcacc
    11701 cgttacgtca ctgaaattct tgttaagacc acgggcaccg tgcagatgct gggacgtgca
    11761 ccacagcaga acgttcaggc gcaaccgaag cctcagcaga atgggcagcc acagagtgct
    11821 gacgcgacga aaaaaggtgg cgcgaaaacg aaaggccgtg gacgtaaggc cgcgcagcca
    11881 gagcctcagc cgcaaccgcc ggagggggag gattacgggt tttcagacga catcccgttc
    11941 tgatcgggct gactgtgaca accgccccgc cctgtgcggg gcatcaccgg agatatgagg
    12001 atgagcgaat atttcagaat acttcagaga ctgccggacg gctcctttac ccgcgaacag
    12061 gcggaagccg ttgccgcaca gtaccggaac gtctttatcg aggatgatca gggaacgcat
    12121 tttcgcctgg ttgtccgtca ggatggcacg ttgatctggc gctcctggaa ttttgaggac
    12181 tgtgccgggt actggatgaa ccagtacatc agggatttcg ggattcttaa gtaagagagg
    12241 tgccggacgc gcgaacgtcc ggcaggacat aagcaattat aaggggatga ttatgcctgt
    12301 aacgaagtgt gaaccagaaa ccacccgcaa agcaagccgt aaatatgcaa aaacgcagga
    12361 aacagtcctg tctgccctgc tggcgcagac ggaggaagtg agcgtgccgc tggcctcgct
    12421 gattaaatca ccgctgaatg tgcgcacggt gccgtattct gcggagtccg tcagcgaact
    12481 ggcggagtcc attaagggcg ttggcctgtt gcagaatctg gtcgtgcata ccctgcctgg
    12541 tgaccgttac ggtgtcgccg caggtggtcg ccgactggct gcactcaaca tgctggcaga
    12601 gcgtggcatc attccggctg actggcctgt ccgtgtgaag gtcattccgc aggagctggc
    12661 gacggcggca tcaatgactg aaaacggtca tcgtcgggat atgcaccctg ctgaacagat
    12721 tgccggattc cgcgcaatgg cgcaggaagg caaaacacct gcacaaatcg gtgatttgct
    12781 gggctattta ccccgccacg ttcagcgaat gctgaaactg gctgaccttg cgcctgtcat
    12841 cctcgatgcg ctggcagaag accgcatcac caccgaacac tgtcaggcgc tggcgctgga
    12901 gaacgacacc gcgcgtcagg tgcaggtgtt tgaagctgcc tgtcagtcgg gatggggcgg
    12961 taaaccggat gtgcgggtta tccgcaatct gattacggaa agtgaagtgg cggtggcggg
    13021 gaacagtaaa ttccgcttcg tgggggctga tgccttctcg ccagacgaac tgcgcaccga
    13081 tttgttcagc gacgacgagg gtggctatgt ggactgcgtg gcgctcgatg ccgccctgct
    13141 ggaaaaactc caggctgtcg cagagttcct tcgggaagcc gaaggctggg aatggtgcgc
    13201 cggacgcatg gagcctgtcg gtgagtgccg tgaggatgcc ggaacatacc gctgtctgcc
    13261 ggagccggaa gcggtgctga ccgaggcgga agaagaacgt ctgaacgaac tgatggcacg
    13321 ttacgacgcg ctggaaaacc agtgtgagga atctgacctg ctggaagcag aaatgaagct
    13381 aatgcgctgc atggcgaagg tcagagcgtg gacgccggag atgcgtgccg gaggtggagt
    13441 ggtggtgtcc tggcgttatg gaaacgtatg tgtccagcgt ggtgtgcagt tgcgcagtga
    13501 agatgacgcg actgatgacg ctgaccgcac ggaacaggtg caggagaaag cgtcagtgga
    13561 ggaaatcagt ctgccgttgc tgacgaaaat gtcttccgaa cgcacgctgg cagtccaggc
    13621 agcactgatg cagcaaccgg acaaatctct tgcactgttg gcatggacgc tctgcctgaa
    13681 tgtgtttgac agcggagcgt acagtaaacc agcacaaatc agcctggaat gtaaacatta
    13741 ttcgctgacc agcgatgcgc cgtcagggaa ggaaggtgcc gcattcctgg cgctgatggc
    13801 agaaaaagcc cgtcttgcgg ccctgttacc ggagggatgg tcacgggaca tgacgacgtt
    13861 cctgtcactc agtcaggagg tgctgttatc cctgctcagt ttctgcaccg catgcagcct
    13921 taacggtgtc cagacccgcg agtgtggtcg cacgtcacgc agtccgcttg actcactgga
    13981 tagcgccatc ggttttcaca tgcgcgactg gtggcagccg acgaaagcaa acttcttcgg
    14041 acacctgaaa aagccgcaga ttatcgcagc cctgaatgat gccggactgt ccggtgccgc
    14101 acgggacgca gagaagatga agaaaggcga tgccgctgaa catgcagagt tccatatgaa
    14161 agacaaccgc tgggtgcctg gctggatgtg ttcaccacgt ccacagacgg atgccactga
    14221 acgcgccgat aacctggctg atgccgcctg atgaacaacc acaccgcccc gccggagacg
    14281 gggcggcaga gggagatacc gtgatgaaaa ctgaactgac actgaatgcc ctgcagtcca
    14341 tgaatgccca ggaatatgaa gaaatccgtg ctgcgggaag cgatatgcgc cgtaatctca
    14401 ctcacgaggt gatgcgtgaa gtggacgcac cggctaactg gatgatgaac ggagagtatg
    14461 gcagcgagtt cgggggcttt ttccccgtcc aggttcgttt cacgccagcc cacgaacgtt
    14521 tccacctggc attatgttcg ccgggagacg tctctcagct ctggatgctg gttctggtga
    14581 atggtggtgg acagcctttc gccgtcgttc aggtgcaaca tatcttcacg cctgtcgcga
    14641 tcagtcacac gctggcgctt gccgcgacac tggatgcgca gggatacagt gttaacgaca
    14701 tcatccatat cctgatggta gaaggaggtc aggcatgagc gtacgttcac gggcactgat
    14761 ccccctcagc gcagaacaac aggccgccat gcaggcggtg gctgtcacag aacaacgtcg
    14821 tcgtcaggga cgcacacttt cagcatggcc ttatgccacc gctttctttc gctgcctgaa
    14881 tggcagtcgc cggatttcgc tgaccgatct ccgctttttt gcccctgcgc tgacgaagga
    14941 ggaatttcat ggcaaccgtc tcctgtggct ggctgccgtg gataaactga ttgaaagttt
    15001 tggggaagtc tgtgttcttc ccctgccatc cgatgcgggg caccgtctgt tcccgtccgt
    15061 gccttttcgt gaaggtgagc gtcgtcgtca gaaaaccacg ctgacagagc agaaatacag
    15121 ccgccagcgg gaacgtgagg cagaacgacg ggaactggaa taccagacat gttttgctca
    15181 ggcgcagatt gaccttgcgt ttcatactcc cgccacggtc ggaagctggt tgtcccgctg
    15241 gtctggtgtt gttgaggagc atgatctgga aacgattttc tgggggtggt gcgggcgttt
    15301 tccatcactg tcatcatttg accggttttt ctggcaggag gaaccactct ggcggctgat
    15361 ttttgaagcc ggtgaggccg gtcgtggtgc accggtacag gtacgtgcac ttgagcagtg
    15421 gatgatcccg aacaagctgg agaacgtaat atgatgaaat cagacgaaaa ataccaggtt
    15481 cccgcgtgga tgcgacctct gttgccgttg ctctgcaaca ccggggggaa cgatccggaa
    15541 gaactgctga atgatacaga aaccactgcc agtgcgaatg ttgtccgtta tgtactgata
    15601 gttgctgtgc ggtcgcaggt tgatctgctg cagcttctgt acaggaaagg gctgttgcgc
    15661 acagagatac caggtggctt ttcaccggaa gaagcgcagg cactcctgga taatctggtg
    15721 cgcagccata tcagcaaggc gctgtcgggc gagcgaatgg cagcccgtga cagaaatgcc
    15781 gatctggcct ggattggaca gcaactggtc gatgccgcct ggtttgtccg tgccacactg
    15841 gaagcgcatg ggatgagcgt cggaaatgag agtccccctg ctccgccgga gacaatgccg
    15901 gacatacaga cacgggaact ggtgatgttg atcaagcgac tggcatcatc gctgaaagcg
    15961 gtgaaaccgg acagttgtgt ggtgcgtgaa gcgcaggact ggctgcgcga cagaaaactt
    16021 gtggatatca cagatattct ccggtgaacc aggcatgtat attttacgca gggacacgcc
    16081 gatcatgatc gtttcatatc aggggtggtc tggaggtcat gcggcgtgtc ctctgcactc
    16141 gccggaataa ggaagtcgcc ggcggctccg cttttacccg gccatgcggg gcatggcctt
    16201 gtgggttttc agctctgtgg cctcagcgtc gtgtgcgggc tgtgccgtgc ctccatctta
    16261 gccgggctgg cagggatgca agggtacgct tcgccgctgc ggtcacccgg tccctccttc
    16321 ccgtctgccg tgattttccg gtcgttcgtc cgctcaggtt tcgcggcctc accctgcaac
    16381 tcccgtcagc cgtgtgcgtc aggctgcggc ttcccttgcg cccctgcatc cccgccttat
    16441 cgcccggctt ttatggaggc acggcaccgc ccggtgtcgc agaacatgtg actatggagg
    16501 attcgggaat gtctgttgtt gcacctgctg tatacgttgg aacctggcac aaatacaact
    16561 gtggaagcat cgccggacgc tggtttgacc tgaccacgtt tgatgatgag cgcgactttt
    16621 tcgccgcctg ccgtgctctt caccaggatg aagccgatcc tgaactgatg tttcaggatt
    16681 atgagggatt cccggggaat atggcctctg aatgtcatat caactgggcc tgggttgaag
    16741 gcttccgccg ggcacgggat gaaggctgcg aagaggctta tcgtctctgg gtggatgata
    16801 ccggtgagac ggattttgac accttccgcg atgcctggtg gggcgaggct gacagtgagg
    16861 aggcttttgc ggttgagttc gccagtgata ccggcctgct ggctgacgtg ccggagacgg
    16921 tggcgctcta ttttgactat gaggcgtatg cgcgggattt attcctggac tccttcacct
    16981 ttattgacgg tcatgtgttc cgtcggtgat ttactccccc gctccggcgg gggtatggcc
    17041 tgc
//
LOCUS       DAOTRK010000104        16977 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.104, whole genome shotgun sequence.
ACCESSION   DAOTRK010000104 DAOTRK010000000
VERSION     DAOTRK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.104"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..4448)
                     /gene="mukB"
                     /locus_tag="QIE41_003260"
     CDS             complement(<1..4448)
                     /gene="mukB"
                     /locus_tag="QIE41_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDS3532114.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPS"
     gene            complement(4448..5152)
                     /gene="mukE"
                     /locus_tag="QIE41_003261"
     CDS             complement(4448..5152)
                     /gene="mukE"
                     /locus_tag="QIE41_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDS3532115.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5133..6455)
                     /gene="mukF"
                     /locus_tag="QIE41_003262"
     CDS             complement(5133..6455)
                     /gene="mukF"
                     /locus_tag="QIE41_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDS3532116.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6452..7237)
                     /gene="cmoM"
                     /locus_tag="QIE41_003263"
     CDS             complement(6452..7237)
                     /gene="cmoM"
                     /locus_tag="QIE41_003263"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDS3532117.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7373..8152
                     /gene="elyC"
                     /locus_tag="QIE41_003264"
     CDS             7373..8152
                     /gene="elyC"
                     /locus_tag="QIE41_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDS3532118.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8129..9022)
                     /locus_tag="QIE41_003265"
     CDS             complement(8129..9022)
                     /locus_tag="QIE41_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDS3532119.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9176..9922)
                     /gene="kdsB"
                     /locus_tag="QIE41_003266"
     CDS             complement(9176..9922)
                     /gene="kdsB"
                     /locus_tag="QIE41_003266"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDS3532120.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9919..10101)
                     /gene="ycaR"
                     /locus_tag="QIE41_003267"
     CDS             complement(9919..10101)
                     /gene="ycaR"
                     /locus_tag="QIE41_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDS3532121.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10153..11385)
                     /locus_tag="QIE41_003268"
     CDS             complement(10153..11385)
                     /locus_tag="QIE41_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDS3532122.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSNFRLIRH
                     RMLAPEKMGWKYKNAWMQEHEAEIAQLIQHIHDKGPVRSADFEHSRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLERC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11422..12408)
                     /gene="lpxK"
                     /locus_tag="QIE41_003269"
     CDS             complement(11422..12408)
                     /gene="lpxK"
                     /locus_tag="QIE41_003269"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDS3532123.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVIWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCLPFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12405..14153)
                     /gene="msbA"
                     /locus_tag="QIE41_003270"
     CDS             complement(12405..14153)
                     /gene="msbA"
                     /locus_tag="QIE41_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDS3532124.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14190..16454)
                     /locus_tag="QIE41_003271"
     CDS             complement(14190..16454)
                     /locus_tag="QIE41_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDS3532125.1"
                     /translation="MKITTVGVCIICGIFPLLVLPQLPGTLTLAFLTVFACVLAFIPV
                     KAVRYIALTLLFFLWGISAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQVTHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGSFDSQRYAIAQHQPL
                     TGRFLQASVIEPDCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPAFRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYKWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16661..16945)
                     /gene="ihfB"
                     /locus_tag="QIE41_003272"
     CDS             complement(16661..16945)
                     /gene="ihfB"
                     /locus_tag="QIE41_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDS3532126.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
BASE COUNT         4067 a         4727 c         4188 g         3995 t
ORIGIN      
        1 gaaggcgctt cgtcacttcc tggaagcgtt tcagggagtt gcggcgcaaa gccacgcagg
       61 ccgacgacgt gaacgtgttc ggtattctgg aagactttac gcaccagttt ataggtggtg
      121 cctttctccg ggctgatatt ttccggcgct gcgatgatga gctgcatttg caaacgttca
      181 cacaattcaa acagcgtggc gatagaacga gcatccagtc gcgctgcttc atcgaggaac
      241 agcaggcggc aaggagagat atctttaccg cgcaggcggc gagattcatc ttcccagctt
      301 tgtaccacca tcaccagaat cgacataccg gtaccaatcg cctcaccggt cgacaatgca
      361 ccagactctg cgcgcaacca gccatcagaa ccacggttaa cctcaacttc catttccaga
      421 tagttgcggt aatccagcag ttcttcgcca atggtctgcg gcgtgcgctg ccccatatca
      481 atctgcggat taagacgctg atacaatttc gccagcgctt ccgagaaggt cagacggttg
      541 ctgttaaaca gatcctgatg ctgctcatgc tgttcagaga gcacatccag cagcatggcg
      601 tgcgtttcac gcacgttcac gttgaggcgc acgctgttca cctgaccaaa cgatacgttc
      661 tgcaacccct ggttgagcat acggatacgg ttctgctcgc gctgaatggt ttttcgaata
      721 atgttcgcca cgctgcggga actgatcgcc agtttctgtt cacgggaggt taactcttcg
      781 gtcagacggc taagttcgat ctccatctgt tcgatagctt ccaccggatc atcggtacga
      841 ataatatcct gacgaatacg ttcacgcaga tgctgataaa ccgccacgaa gaactgaatt
      901 ttacgttccg gacgttttgg atcttccgac atgcgcagca catcgcgcag atgttcgtta
      961 tctgccactg ccagacgcag cgcacctaac gccttatccg acatggaacg caaatcatca
     1021 gcggagagat aagccagctc acgacggtgt aagcggcgct caacgccgtt atctttcacc
     1081 atgcgcatga ctgcacacca gcccgctttc gcggtcacta cctgctcgcg catctcaaag
     1141 taatcccgct ccagcttgcg cagtttgcgg gtcaggttgt ccatctctgc ttcgcagaag
     1201 gtaagcgctt tttccagttg attgcggcgt gaacggttat tgctcagttg cgcgtgcaac
     1261 tcgtcacggc gaatacgcgc ccgctcttct gccccgctat cagctcgcac gccgatatcc
     1321 tgcaattcac gttgcagatc attgagtagc tcttttttgg tgtcgtaaga acttttcagc
     1381 gaagccagca cctggttgta ctgactcaac tgcgctgcgt gtccgcgcaa cgcttcacgc
     1441 gcgcgggtac gctccgcttc cgcctgttcc agacgttcac gcagtttttc gttgagatcg
     1501 ctgttaccgc taagcatttc tgccgagtca gagtagctaa agtgcgcacg acgctgcacc
     1561 acttccgtca gggcaaacgc ctgctgacgg gcatcgcgct gcatctgctg agagtacgcg
     1621 taatcttctt ttaactgttc gaactgttcc gggtcactct gcaataccga aacgatcggt
     1681 tccagtttcg ccagttgatt accgaactgc tgaacaaaac gcgcggcttc ctgggcttca
     1741 tccagacgtt cgcggatttc atcgacgcga tccgccaggc tgtcatcagc caacaagttg
     1801 agacgcggca gaatgcggtt cagcgccgta acaccctctt tcgcctgctc aaactgaata
     1861 cgctgctgct ggttatcatt ttcatgatta ctcaacgccc gctccagttc gacgcgacgg
     1921 ctgttcagtt gacggatttc tgcttccggg tcagactcaa acgccaccgc cagatgacta
     1981 cctataaagc ggctgaatgc ctgatgcaga cgctgggttt tctgtacatc aaaggagagc
     2041 gtggcgaagc gttcggaaag cacttcacgc tcggcatgga ggctttcaat acggctttca
     2101 cgcgcagcac gaccaaacag cggcacttcc gggaaacgtg aataacgcca ctgacgatcg
     2161 gcgattttca ccactacagc tttttccagc tcatcaacgc tgaacacgct gtcatcgaat
     2221 gactgcggat ccccttcgat cagatagaga tcttccgggc aatcggtcaa gccttccagg
     2281 tgctcggtta cctgtgacag atctggcacc acgatggcgt ggcgtgacgg gccatacagc
     2341 gctgagaagt acggcgcatc ttccaggcta acgtcatcat aaatttctga cagcagcaca
     2401 ccaccaaaac gctccgccag cgcgttcaga cgctgatctt cagagccgcc tggctggctt
     2461 aaacgttcga tctcttcatc gacggcgttt ttgcgtgcgc caacttcgtc acgttcaaca
     2521 atcgcttcgc gctcacgctc aagcaactgt tgcagatatt cggtgacgtc ctggctggag
     2581 gtgaactctt cgccgcactg ttcgctcaac tggttgagac tgttttgcgc tgccagccaa
     2641 accggcgcac gctgcatcaa actctgaatg cgagactgca gctgttcctg ttcctggcgc
     2701 agtgccatgc gctcttcgcg ggcgttagac acgctatcgg aaagagaggc aatgcgtgct
     2761 tccagctcct gatgcagagc ttccagttca tcaatatcaa aattcttgcc ctgacgtttg
     2821 cagaaatctg ccagcaggcg ttcggcttct tgctgctcgc gcagacgctg ttccagttcg
     2881 cttaagcgca tccgcaacgg ttgaacctgc tctgccaggt gacgctgatc gaccccttcg
     2941 cgcaatagtt cgcgagcgac atcccacgcc tcgttacgcg ccagcgggcc attaattgcc
     3001 accaccagct gataagcctg ctcaaactgg ctgtgcgcgg tttgcgccat gctcattttc
     3061 tgctcaagag agagcatttt ttcagtcgct tccagctctt tcgcctggaa ggtttccagc
     3121 cattcggcgg cgctgtcggc ggttaagtcc ggcagatggc acagttcttt ggcacgatta
     3181 agcgcagcaa tcgcctggtt atactggatc gcgcgcgttt gctggacgtc cagcgcctgc
     3241 tgatagtcag caagctggct tttcagctcg tccacttcca gttcggcagc ttccgcacga
     3301 gcctcgttct cttcctggcg ttcgatggct tctgccacca cttcgttttg ctcttccaga
     3361 cggatctgca gctcatcgag atccgcttcg taacgttcga tcttctcctg ctgacgcagt
     3421 gcggtttgca ccaggttcag gtgatcgctg gccgcctgat aatccgcttc cagatcgcct
     3481 tcggcaccgt tgtgctctgc cagctcacgc gccatatcga cgtgtttgta ctgctcagcc
     3541 gccaattgct gacgcgaagt atgtagctcg cgacgaaatt ccagggcttt gtcgagatgg
     3601 acacggcgct cgttggcgtg acgcatgtag tccgccgcca cgtagttggt ggcttcgctg
     3661 atcagatgct taaacaggtc acggtccgac tgggtgacac gaatcgcttc cagcgtcatg
     3721 cgattttcac gcagtgccgc ttccatgtcc tggaacgctt tacgcacccc gctgttttcc
     3781 ggcaacaggt agtcgcgcag agaacgagta atggcgctgg agatcccgcc atacagcgag
     3841 gcttcgatca gacgatagaa tttgctacgg tcagatgcgg agcgcagacg acgcgcgatg
     3901 atgcccagat cgaacatcag cgagtggtaa tcggtaatgg agttgaactg tttaaactgc
     3961 acgccttcca tcgcctcgag cttgtctttc agctcgttaa gcggcagcac gcgcgcctgg
     4021 cgttcgttca gggtttcggt caccaactgc gtcggcagca ccgacatcgg caggccctga
     4081 atggcaaatg gtttgatatc cactttacga tcgcgtccgg caacctgttg cagacgcaca
     4141 ccgaccacca cgcgctggtg gcgcgagtta atggtgtcga gcatcgaata acagacaccc
     4201 gctttcagct taccgtgcag acctttatca cgcgaaccgc tggtggcccc ggcttccgta
     4261 gtgttacgga aatgcagcag ggtcaggtcg gggatcagcg ccgtaacgaa cgccgccatg
     4321 gtggtggatt tacccgcccc gttaccgcca gaaagcgtcg tgaccagctc gtcaaggtca
     4381 aaagttcggg caaaaaagcc gttccagtta atcagcgtca gtgagcgaaa tttaccgcgt
     4441 tcaatcatta ttcttcctct ccgctatctg gctgactctc ttcggtttca tcgttgagtt
     4501 gcagatgatt ttcaatcggc attgcttcgc catcacgaat caggcgacgc tgtgcttcac
     4561 ggggatcgtc gccagcacgc acatcggctc cgaagcggaa caccgattcg gtaatgcgga
     4621 acttgctgct gtcgtggccc ataaaccaca ccatgcccaa acgacgcaga cggttgagcg
     4681 aagaacgtac tttctcctgc aacttctgcc ggtcaacgtc tgaaccggtt gaacggttgt
     4741 tcaccagttt cagcagtttc gcttcatcgg ccagggtgag cagttcgtcg tacagttcct
     4801 gttgagtaaa aatcccctca ttcgccagcc gttccgggct gagatagaga taacagagga
     4861 ttttcccgac catcatatcc agttccgaca agacggaacg agggatcagc gtggtggaac
     4921 gtgggcgtaa atagaagaac ccttccggtg cacgaataag ctcaacgtta taacgcgcgt
     4981 aaaactcttc cagatattcc tgaaaatcca tcaggaatgc atgattatcc agttcgtcga
     5041 ggccaatatg gcggcctgaa cgtaaggcgc tatccagcgc cggaaataac ggattcgcca
     5101 gcgcctgcgc cagcttaacc ggcatcactt gttcaatatt tgtcgatgac atgcgcctgt
     5161 accttggctc cgtaatcatt aatcggctgc catttcgctg gcagtccggt gaaatctgct
     5221 tgcgctacgc caagacgtac cgcctgatca ataacaatgc gcgcaacgtc aaagtgacgt
     5281 gcacgcggat actgtgacag atattcgcgt accaccagac caagatccag cggcacttgt
     5341 ctggttttgt acacggcgag ttgttcttcg atgatcgccg ccagctgttc gcggatctcg
     5401 ttaaactctt cgtactccag atcctcagga agttctcctg tcacttcttc atcgcgcaga
     5461 gccatctctt cgtcacgcat atccaacaga cgatcggcat tggcataagt tagcgcccac
     5521 ggctcatcaa aataggtttg taccgactga cgtaaccgct gagcaaagac gcggttttta
     5581 tccatatcga ttgcggtacg gataaatttg tgtacgtggc ggtcgtagcc aatccacaag
     5641 tctatggatt gctggcccca gctgataata cggtcgagtt tgctctgcag atcgaatacc
     5701 agacgatcga caaaatgcag atcgtcatgg gtcatcgtcg catcctgaat gcgcaacaga
     5761 ttagcctgca atttgtcgcc tgccgcttcc agcgtatcct gcaattcacg cagcgttccg
     5821 gaagtttccg aaagcaacaa ttcacagctg gagatcgccg cccgccagtc tttgttcagc
     5881 aactgggcga tatcgtcctt cacctgctgc tgctgttcgt ccatcagacg ttgcgtcagg
     5941 tcgatactgt caaaaatttc tgctaccgaa tatttcagcg gcgcatagac attacggtgc
     6001 cagtgaaatt catcaccgcc ctcttcggcg gcatctgctg cgcgtttgag ctcacccgcc
     6061 acaatcgaca actgcataga aaggcgcagc gtagaaaact cgcgctgacg gatgtaatag
     6121 tcagtaatgc cgatgccgag cggcgtcaga cggtaaattg cgttcccttc cgcctgctcg
     6181 ctggtaaagc ggttcagcag acgttgacgc accatgtcgt tgatcgcgtt gttggcgcgc
     6241 acgccgatgg tttcgctggt ttgctcaaac gcatcactca catggcggaa tgcatccacc
     6301 agctcgcctt cactcatctc accatccaga cgctcgccgt tcagcgtggc aaccgccagc
     6361 agaaaagaga gtcggtctac cggcagcgag atggagaagt cattttttct ggcccaggca
     6421 accagttcgg ggactgtctg ggaaaattca ctcatacttt atccttgctc tgcggtttgc
     6481 gcgcggtgac atgaatataa cgccccaggg taatatacgg ttcctgacgg caataacgcg
     6541 tttctaattc aagtaatgct tcatagcagt cgcgctgctg gtgcttctcg cgcagataat
     6601 catgaaacac gcgaacgcct gtcttaccca taatttgcca accagcttct tccagccaca
     6661 gataaacctg cgccgggtcg cgtggataat ctggcgaaag cgtccgtttt ttctttttcg
     6721 gcattcccgc ctgcacgtaa tcaaaattcc cggcgaccat gttatgcatc agcaagccgt
     6781 gcgcattgta gaacattaac gacaacacac cgcctggacg caacactgac cacagggtct
     6841 gtaatacgct gcggggatcg gccacccact cgagcaccgc atggaacaat atcagatcaa
     6901 cgggcgtttc caaatgcgaa gcaacatcct gagcggcgca atgtataaat tgcatgttgt
     6961 cgctcacacc ttttgcttct gccgcctgtt ttgcgcggtc gatcatctgc gcagaaagat
     7021 cgcataaaat gacctgatgc ccacgctcgg ccattttgat tgcggtctgc ccttctccac
     7081 cgccagcatc cagcacacgc agtttttgcg ggcccatttc cgccagcacg cgatcgagat
     7141 cctgccacag aatagcctgt cgaagctgcc ctttggtggt gccgtaaatg ttacgggaaa
     7201 acttttccgc aatatcatca aaattgcgat cctgcattgc tttctccacc ggttggctaa
     7261 gacgttttct gataaagacg ctattttgtc acagccacgg cgagaatgaa cctgtctggt
     7321 gtaatatcgc tggcgatccc ttgctatatg gttaaaaaag gaaccagaaa ggatgctttt
     7381 tacactgaaa aaagtgattg gcaatatgtt gctgccactt ccgctgatgt tgttgattat
     7441 tggcgctggc ctggcgctgc tgtggtttag ccgttttcaa aaaaccggga agatttttat
     7501 tagcattggg tggctggcgc tcttgttatt gagtttgcaa ccggtagccg accgcctgct
     7561 gcgtcccatc gaaagcacct atccgacatg gaataattcg caaaaagtgg actatatcgt
     7621 ggtgctcgga ggtggttata cctggaaccc gcagtgggca ccaagctcga atttaatcaa
     7681 taacagtctg cctcgcctga atgaaggtat tcgcttatgg cgcgaaaatc cgggatcaaa
     7741 actgatcttc acgggaggcg tagcaaaaac caatacggtg agtacagcgg aagtaggtgc
     7801 cagagttgcg caatcgctgg gcgtgccgcg cgagcaaatt atcactctgg atttgccaaa
     7861 agataccgaa gaagaagctg cggcagtgaa acaggcgatt ggtgacgccc ctttcctgct
     7921 ggtgacctcc gcatcacatc tgccgcgggc gatgatcttt tttcagcagg aagggttaaa
     7981 tccgctacca gccccagcga atcagttggc catcgactcg ccgcttaatc cgtgggaacg
     8041 ggcaatccct tctccggttt ggttgatgca tagcgatcgt gttggctatg aaacgcttgg
     8101 ccgcatctgg cagtggctga aaggtccgtc aggcgagcca cggcaagagt gattttgatg
     8161 ccagatcgaa gttgcgccga ctaaatcgtc cggtattaac cagttgcgca acttcatccc
     8221 ataacacata caaccaacgc cgccagatga acgactccgc cactggcgcg cgttgcaggt
     8281 aactccagag caaatcttcc gccagagaat tatccattag tcggaacagt tcgtattctc
     8341 gcggtgccca aagcattagt cccggcccga ccatcgccag taactgatcg ctgcgcgaat
     8401 ctttcagcat gctgcgtaaa cagaagttac cgtgaatcag cacacaattg tcgttaaagc
     8461 cttcaaataa tgccggaaga cattcgcgag tacgaaacag gatccgctta tcctgcatcg
     8521 ttagaccggt gttattgaac tgattgagcg tggtccatag cacttcaaca tgttgccggt
     8581 accatgaggg ccagaaattt tcctgagtat tgtcgaccgc gccgacgcaa ccgcgactgt
     8641 cctgacggtg ccaggccagt aaggcttcaa cgatttgatc tttgagctgt tcccagcgtt
     8701 ctggtgttcg ggctggtgcc tccaccgaaa caccacgcat ccgctccagt agcaggacat
     8761 cggggccggg gtgttcttca tgcgtcatca caccataaat agtcggcata cggacagttc
     8821 cacttcttgc cagcatggtg gttttccatg ccagttgtcg ggcttttccg ggcgtactaa
     8881 agcttcttgc cattaacggc attgggtttc cctggctgtc atacaaagcc cacaacgccg
     8941 tatccgcttt ttcattgacg cactcaatac gactgagttt ttcgcccagt aaatggctta
     9001 attcggcacg cagctgttcc attcagatta ccctcataaa agctactgct ttcataatga
     9061 gcgccaatat aaaagatgtc acctgaatga gatcaattgt tgaaaagaaa aattgaggaa
     9121 aatcgtaacg gtacgacact cctcccaaaa ttggctggag tgtcgtgaag tgaaattagc
     9181 gcatttcagc gcgaacgcgc tcgagatctt caggggtatc cacacctgtg ccaggaactt
     9241 cctgggcgac agcaacatgg attttttcgc cgtaccacag aacacgaagc tgctctaaca
     9301 tttcgatgtg ttctaacgga cttggctgcc agttgacgta acgacggata aagcctgcac
     9361 ggtagccata aataccaaga tgacgcagga agttatcgcc aacggtttca aggccttttg
     9421 caaaacgatc acgatcccaa ggaatggtag cgcgagagaa gtacagtgca tacccttcag
     9481 cgtcgaggac cactttcacc gcattcgggt taaacgcttc ctccgcattg tggattggca
     9541 ccgccagagt cgccataccc acctgacgct gggcgagatt atcagcaacc tgacgaatga
     9601 ttgtcgcagg gatcatcggt tcatccccct gcacattaac gatcaccgtg tcgtcgctga
     9661 atgcgcattt ttcgacaact tccgccagac gttctgttcc tgactgatga tcggcgcgcg
     9721 tcatacatac ttcaccgcca gcggcttcaa cagcgcgagc aacatcctca tgatcggttg
     9781 ccacgatgat gcgctcggca cctgattcgc gcgcgcgttc aagaacatga acaatcatgg
     9841 gtttgccgtt aatatcaacc aatggtttac cgggcagacg cgtggacgcg tagcgcgcgg
     9901 gaataatgac cacaaaactc atgatttact ctcatcagca gtcagcacgc gggcttccgt
     9961 ttccagcaac actggaatgc catcgcgcag cgggaaagcc aggttgtcca gtttgcaaat
    10021 aagttcttgt ttttcctggt tataccaaag ttttccgttg caaaccgggc aggcaatgat
    10081 ttcaagcaga cgatgatcca tagttcctcc agatggatcg ggttatgaat gcataaatct
    10141 tatcataatc atttatgcga cggggtctat ttcccagccg gtgcggcaat cagtaaagag
    10201 gccttgcggg cagcgttcta atgtcacccg cgttgcctgc tgccagttag cgaaatcagt
    10261 aatcgcctga cgtaaccctt tttgcagcgt tgtcgttggt ttaatgcctt cttgtaacca
    10321 cagagagata acttcaagga tgcctgtctg gcgatgcatt ttggcatcca ttcgcccaac
    10381 taattgccca cgatgtaata acggcagaac aaaatagcca tactggcgtt tcggcgctgg
    10441 ggtatagcac tccagccggt agctaaaatc aaaaagctgc tctgcgcgtt tgcgatccca
    10501 gacaacagga tcaaaaggcg aaagtaccgc gctgtgcgtt gcagtgagct ttccggccag
    10561 cgcacgctcg agtagcggca gcaaatcagc atgcagccaa agattgccca atttttcaac
    10621 atgcacagca atgatttgct gctgttcagc cctcgcttcg cgccacgccg ccagtgccgg
    10681 acgtttcagc cgatagtaat ctgccagcca ctgttcgcgg aatattccca ggctacgcgc
    10741 actgttatcc agcatgatga tttctgcttc tgtttgcgaa acgagatcgc gctcatcatc
    10801 ccagtcaggc atgactcggt gggttaaatc ataaacgcgc tggaagttgc gccgttcaat
    10861 caccatcacc tttccggcag taaataaacc ttccagatgc cgtttatgcg gcttccattc
    10921 ccaccagccg cttgcacctt tacgagaatg ctcgaaatcg gctgaacgta ccggcccctt
    10981 atcatgaata tgctgaatta actgtgcaat ttccgcctca tgttcctgca tccaggcgtt
    11041 tttgtatttc cagcccattt tttcaggtgc cagcatgcgg tggcgaataa gacgaaagtt
    11101 gctacgcggc ataaagcagg cttcatgcgc ccagtattcc attaattcgc cacgcgccag
    11161 agactcatcc agccactggg caggataatt tcccagacga ctgaaaagca ccagatatgg
    11221 actacgggca acaatattaa tggtatcgat ttgcagcaag gacatgcggg agatcgttgc
    11281 cggaatatcc tccaacgatg ctcgacggcg gggtttgttt aacaggcctt gcgcggcgag
    11341 gtgaagatta cgcgcatcag caagagagag gtgcggcagc gacattcatg actccatcaa
    11401 tcgaacgctg ccgcggcgta actagttgcc agaagccagc gaggttagtt gcgcaagcag
    11461 tttcgctggt tcatcacctg aaagctgtgc gtcaacaggc aaataccacc aatttccttc
    11521 ggcaaagggc aggcatttca ccgcatcttt ttcagtcatt accagcgttt gcccggcgct
    11581 taccaacgca ctaacatccg catggttcaa agactgatga tcggccagtg gtacacattt
    11641 ttccggttgt acgccgcaca tcttcagcgt ggcaaaaaag cgcggcggat gtccaatccc
    11701 cgccatcgcc actacatgtt caagctgagc aacgtcacaa cgcgtaccgg tacgtaaatt
    11761 caccgcctga cccggcagca gatgcatggg gatttcaccg ctgcggggga caccgccgtt
    11821 gacgattacc gcatcaaccg actttaagcg ccccgctcgc tcacgcattg gccccgccgg
    11881 caaccaccaa ccattgccaa agcgacgcac accatcaata acgacaattt ccacatcacg
    11941 cgccagacgg taatgctgta aaccgtcgtc ggttacgatg atctgcacat cagggtgttg
    12001 cgccagaatg gcttttaccg catcagaacg cacgggagaa accgcaacgg gcgcgccagt
    12061 gcgttgataa atcaacacag gttcatcacc cgcctgtgct gtggtggtat ctgccgacaa
    12121 taacagcgga taagattcag ccttaccacc atatccccgc gatacgaccc ccacgcgaat
    12181 accgcgctgt tgcagctgtt ccaccagcca gataacgacc ggggtttttc cgttgccgcc
    12241 tgcggtgaga ttaccaacca cgacaaccgg tacgggggca cgccaggcgc gcttcagttt
    12301 tagtttatag caaagacgga tcgcgccact caccaggcca tacaaccagg agagtggcag
    12361 caatagccgc cacaaagggg attcaccaga ccagattttt tcgatcattg accaaactgc
    12421 attttgtgaa gttgcgcgta aacgccacgg tgctcaagca aatcgttatg cgtaccgcgt
    12481 tccacaatga caccatcctc gacgaccacg atttcgtctg ccttttcaat ggtagacaag
    12541 cggtgggcaa tcaccagaga ggtacggttt ttctgcaact catccagtgc cgcctgaatc
    12601 gcacgttcgg attcggtatc caacgccgag gtagcttcgt ccagaatcag aatcgggcta
    12661 tcacgcaaca aggctcgagc gatggcaata cgctgacgct gaccgccaga gagcagcacg
    12721 ccgttttcac caatcactgt atcgagaccg ttatccatct tattgatgaa gtccatggcg
    12781 taggccatac gcgccgcttc ttcaatttgc tcacggctgt actgttcagt ccgtgcgtaa
    12841 gcaatgttgt tggcaaccgt atcgttaaac agatggacat tctgcgacac cagagcaacc
    12901 tggttacgta acgacgccag ggtatactcg cgcagatcgt gaccatccat caggatttcg
    12961 ccttcatcaa tatcgtaaaa acgtgtgatt aggctggcga tggttgattt acctgaacca
    13021 gagcgtccaa ccagagcaac cgtcttccct gccggaattt tcaggttgat gttacgcaat
    13081 gcgggcacgt cacgtcccgg ataagtaaag gtgacattgc ggaattccac gtcgccagtc
    13141 gcacgctcga tcacgcgctt accttcgtct ttctcctgct cactgtccag aatggtaaac
    13201 agcgtctgac aagccgccat accgcgctgg aactgggcgt taacgttggt cagcgatttc
    13261 agcggacgca tcagtgcaat cattgaagag aaaacaacgg taatcgtacc ggcagtcagg
    13321 ctatccatga cacttgggaa gctcgccgca tacagaacaa acgccagcgc caaagaggcg
    13381 atcagctgaa tgatcggatc agagatggaa gaggctgaaa ccattttcat cccctgaaga
    13441 cgcattcggt tactgacttt atcgaagcgt ttcgtttcca cttcctgacc accgaaaatc
    13501 aatacttctt tgtggccctt cagcatttgt tctgcgctgg tggtcacctg ccccatggtg
    13561 ttctgcatgt ttttactgat gttacgaaaa cgcttcgata caacgcgaat cgcaatcgaa
    13621 acaatcggtg ccagcacaat caaaatgatc gacagttgcc agctgtaata gaacatcatg
    13681 atgaacaggc cgatgatcga cgcaccttca cgcacaacag taatcagtgc gccggaagaa
    13741 gaagaagcaa cctgttcgga atcgtaggtg atacgtgaca acagcgtacc cgttgactgt
    13801 ttgtcaaaga atgaaactgg cattcccatc atgtgaccaa acaggcggcg acgcatggtc
    13861 attaccacct ttcctgatac ccaggagata cagtagctgg agacatagct ggtgatacca
    13921 cgtaaaatca ttagcccgat caccaccagc ggcatccaca ccagcacgga gcgatctgtt
    13981 ttaccaaagc catcatcaag aagtggctta aggagcgata acatgaaggt atcgctggct
    14041 gcgttgagga ttaacgctac gcccgccacg atcagacccg ctttgaaagg cgcaatggtt
    14101 ggccacagtc ggcggaatgt ctgccacgta gagagatctt tgtcgttatg cattcaaaaa
    14161 accagcattt gttgaaatag ccgcatattc tacccgttat ccactggcac gccaaaccac
    14221 tgatgatacc aacgaggtaa aatttgctcc ctgaggctgc taatccgcca gccttgcgct
    14281 gaaaaattga ccgtcacttg accctgatgt ggagtatcaa gccacttata accttgttgt
    14341 tgatagcgat gcttaacttt gttggagggc aatcgccatg cgttatagcg cgatgccgat
    14401 gcgagtgcca cttttccatt cactcgctga attaatggca acgatgatga ggtattactg
    14461 ccatggtgag gtacctgaag caatgttgcc tgcacttgct gccagtaacg gcttagcatc
    14521 ttttgttcag cgggggcttc aatatcaccg gttagaagaa tgctattcgt cccgtcatca
    14581 accttaacca cacaggaatg gttatttcct ttatcgttgc tagcttgtaa aggccagtgc
    14641 gcgctgaaac gcaatccttg ccattgccac gcttcgccac gcacacaggg ctgatgatgt
    14701 tcccagttta acggacttct gatccataac atcggccatg tgtgcaatat tgagtccagc
    14761 cctccccggt gatccagatg ttcatgactc agaataacgc cttccggttc aagattatgc
    14821 cagtggagcc aggggataat cagttgttgc ccactatccc cttcgggcca ggccagtccc
    14881 gtgtcataga gaatcgcttt gccgtttcgc gcaatgacca ttgccagccc ttgcccgaca
    14941 tcaagcatgt acacttgcca ctcgtcaggt cgaggttttt gccacagcgg ccagcacatc
    15001 agcaagcctc cagccacaca cattgctggc aacgttcgcc aggcgtttaa tcgccatacc
    15061 accagtaaga accatgggga aaatgttagc cattgccaac gttcagcaat gttgatccac
    15121 ccttccggca aactctttaa cccccagaaa agtaaagcca aagaccggtc ggcaagaaac
    15181 cataacccct gttccaggat taacggcccg cttaaatgca caaccatcgc ggcgaggatc
    15241 aatggaaccg tgataaatgt caccagggga attgcaaata gatttgcaat aaacgaggtc
    15301 agactaatgc catgaaatat gacgatttgc acgggcataa gcaggagggt gattcccagt
    15361 tgcagatgga tgagggaaac aaccgcacgc aataccggcg gcagctgcca ctcaggacag
    15421 ggaaaccact gataccaaaa aatcagtgcc gcgaccgcag cggcagagag ccataaactt
    15481 tgcgagagaa tggcaacagg atccatcagc aaaattgccg ccagacaaca tatccataca
    15541 tcccagccac tccattgtcg cccacttaac ttaagcattc cccacatggc aagcgccacc
    15601 acggtacgaa atgcaggagg ttgcattccc gtcagccagg cataaaaagc agcacagcag
    15661 attccgccaa ttaatggcat ttgccagtgg atccagcgcc caggcagaaa aatttgtcca
    15721 ctgcgaatga gtccggcagc cagcaacgcc gcaaaagcga tatgcaatcc cgatatcgcc
    15781 attaaatgcg ccgttccagt atcgcgcatg atatttttga tttctttggg aacggataac
    15841 cgttccccca tacctaaacc aagaataacc gcattccacg gatagggttg cagcgttgtt
    15901 tgtaatgacg ccagatactg tgcacgcaag ctacaatccg gttcaatgac acttgcctgc
    15961 agaaagcggc cggtcagcgg ctgatgctgg gcaatggcat aacgctggct atcaaagctg
    16021 ccatcattaa gttggccatg aactgcacga actttgagtt tcattgacca tagttgtccg
    16081 gcacaaaccg cttgcgggag atattcgcca tacagcacga ggcctggcgc agggaatata
    16141 cgtcgacctt gtagatgagt gacttgaccg taatgagtgg tcatgccgtc agtagcagtg
    16201 atctcaacta ttgcatcctg cgtcgcgcca gttaaggttt ctcctgccca caaaatttgc
    16261 tttgccgctg atatgcccca aagaaaaaac agcaacgtca gcgcgatata acggacggct
    16321 ttaacaggaa taaatgccag tacgcaggcg aagacagtca gaaacgcaag ggttaatgtc
    16381 ccaggcaatt ggggcaaaac cagcaacgga aaaattccgc aaattatgca tacgccgact
    16441 gtcgttattt tcatgcaccc ctccgtattt atggaaagtg tgcaactttg ttgagcggtt
    16501 gtctggtggg agttttgcgt tccccgaggc acgttcagaa gaatttaacg agagggtgaa
    16561 aactgttcaa aactctgcga ggcagcttcc agttccagat tagagaaatg aaaaaagcac
    16621 ccgacaggtg cttttctcgc gttcaagttt gagtaaaaac ttaaccgtaa atattggcgc
    16681 gatcgcgcag ttctttacct ggtttaaagt gaggaacgta ttttccttcc agttctactt
    16741 tatcgccagt cttcggatta cgtccggtac gtggtgcgcg gtagtgcaaa gagaaactgc
    16801 cgaaaccgcg gatttcaata cgctcgccct gcgcaagagt cgaggccata tgctccagca
    16861 tctcttttac tgcatcttca accgtcttgg cgggaatgtg cgattgctgg gtggcaagtc
    16921 tttctatcaa ttctgacttg gtcatgattc ctccggttcc ttaaaggcaa attaagc
//
LOCUS       DAOTRK010000105        16576 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.105, whole genome shotgun sequence.
ACCESSION   DAOTRK010000105 DAOTRK010000000
VERSION     DAOTRK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.105"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            626..816
                     /locus_tag="QIE41_003273"
                     /pseudo
     CDS             626..816
                     /locus_tag="QIE41_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(886..1026)
                     /gene="ykgO"
                     /locus_tag="QIE41_003274"
     CDS             complement(886..1026)
                     /gene="ykgO"
                     /locus_tag="QIE41_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDS3532127.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1026..1289)
                     /locus_tag="QIE41_003275"
     CDS             complement(1026..1289)
                     /locus_tag="QIE41_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDS3532128.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1648..1749)
                     /locus_tag="QIE41_003276"
     CDS             complement(1648..1749)
                     /locus_tag="QIE41_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532129.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2222..3364)
                     /locus_tag="QIE41_003277"
     CDS             complement(2222..3364)
                     /locus_tag="QIE41_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDS3532130.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASNVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRLEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            complement(3538..4458)
                     /locus_tag="QIE41_003278"
     CDS             complement(3538..4458)
                     /locus_tag="QIE41_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS3532131.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALEKLIPFYKTHL"
     gene            4615..5541
                     /locus_tag="QIE41_003279"
     CDS             4615..5541
                     /locus_tag="QIE41_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3532132.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            5741..6634
                     /locus_tag="QIE41_003280"
     CDS             5741..6634
                     /locus_tag="QIE41_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3532133.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARTLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPQTPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(6665..7654)
                     /locus_tag="QIE41_003281"
     CDS             complement(6665..7654)
                     /locus_tag="QIE41_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS3532134.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFATQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(7681..8532)
                     /locus_tag="QIE41_003282"
     CDS             complement(7681..8532)
                     /locus_tag="QIE41_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS3532135.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNKVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIASLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            9097..13347
                     /gene="fdeC"
                     /locus_tag="QIE41_003283"
     CDS             9097..13347
                     /gene="fdeC"
                     /locus_tag="QIE41_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDS3532136.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTGDNNVEKNVASFAANAGTFLSSQPDSDATRNFITGIATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKEQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVLRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTLTAIWSTKDANDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASRAVISDMAVKVNNQLANGQSANQVTLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGTLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSDAQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLLKDSEVTLTASPANLALDPDGTATTNEQGQA
                     IFTATTSVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(13472..14329)
                     /locus_tag="QIE41_003284"
     CDS             complement(13472..14329)
                     /locus_tag="QIE41_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3532137.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            14577..15446
                     /locus_tag="QIE41_003285"
     CDS             14577..15446
                     /locus_tag="QIE41_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS3532138.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAKAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(15606..16199)
                     /gene="rclC"
                     /locus_tag="QIE41_003286"
     CDS             complement(15606..16199)
                     /gene="rclC"
                     /locus_tag="QIE41_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDS3532139.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(16211..16447)
                     /locus_tag="QIE41_003287"
     CDS             complement(16211..16447)
                     /locus_tag="QIE41_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDS3532140.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         4503 a         3595 c         3998 g         4480 t
ORIGIN      
        1 agtgtcaata tgtgaacaga aaaggaattc catggttaag tcaatattta caaaaaatga
       61 aaatattgct tttttgataa gaagaaaaag acattcagtt tcataaggtt attggcaata
      121 taacgttcaa ttcttacttg cattttatat tagccaggaa ctttgccctg aggtgttagg
      181 aatagtctta atgtgttgtg taatggacta gtaaaaaaac aagtttaaat gataagaaat
      241 agaatataaa actatcatga gtcataatta aagatgattt gccaatcagt aaggtgtttt
      301 aatggattaa catgccgcac aaaatggtta tgggtgttca tcccaataag tgatgaaaaa
      361 ttaaacaggt gtaagtacgt catctgtccg gtaagccccc taagagccaa attaagtgaa
      421 gttaaacgaa gactgccagt tgtcacagga acgatggcct ctttgccagg gcactgcatt
      481 cagtcatctg tgcgcgttac tctaactggc actcgaacgt cggcgatggg gttaccggtg
      541 gatcaggccg ttcctgtggc gtaaacgttt ttatgtcagt accaacagat ttaccacatt
      601 tatcatctta accgtttgag tgtaaatgca ggctgcgtcg caaagggagg aaactgagtg
      661 gcgtgttcag tctaaaagag agttgatgcc agcatacagg gcgcacctgg tcaacaagtc
      721 aacattaata taatggaata ttcggagaga aacgtcaggc agttgacctt aaatgaacag
      781 gaagatacat cctcaggaaa attaatgctg gtgtaataaa tacaccaaca ttaatccggt
      841 ctgattatta atcagacaaa tatcgttaat tcgtcgaatt ttgaatcaac gttttttctt
      901 acgaccctga acggccttaa aacgtggatt agatttacaa atcacatata gccgtccttt
      961 tcgcttcaca atctgacagt ctggatggcg ttcttttgcg gtacgcagag agttaaggac
     1021 tttcatcatg cccccttttt cgtgctaaca aaacgaccaa aacgttgggt gaatcgtgca
     1081 acatttcctt ctgatgccac tgttctcagc ttccctgtat agaacgggtg cgatttagaa
     1141 gagacatcaa ttgtcacgta cggatacgtt acgccatcca gctcaatctc acggtctgtt
     1201 ttgatagtcg agccgatttt aaagtactca tcaacactgg tgtcgtggaa caccacagta
     1261 cgatactcag gatggatatt gggcttcatc atttttacct gttatgttat aacataacca
     1321 taaagataca tgctcatccg gatcatttca acacccaatc cctgtgacca tttgtggtat
     1381 gtggaatttc ggcaacgggg aagtctggca ttgttagcag ccattggcga cgctcccata
     1441 aacgccttga tgagtgatgg tttcgctgag agtatgaatt tttcgagaat ggaggctatc
     1501 tccattttac tggccgatga tttatcgcag tttgattgat tgccggacag cgtgtattcc
     1561 tctcccacgg atataataat tacttacaaa aaagggagag gatgcatatt ttttaatatc
     1621 actgaagtga acagtttatt gccgttatta atagaaatgg agaaataaat aggcgtattc
     1681 tacaattgcg acaaaaacaa cgatattaat cagtttatga ctgatttgtt gtactttatt
     1741 ctctttcatt ggtacttcct cgctttaaaa aagagtgcac ttcgtaagtg cccttatata
     1801 aataacgagt ttggtcaacc aattttttga catgtatcac aaatttgaat agatgtatta
     1861 catcggctat cttttattgc cccaacgtca ttgatatatg tcgcctgaag tcagtccggg
     1921 aatgagtctg atctcaaaac tggcccagcc cgggcgttga ttggcgctga ggagcatatc
     1981 gcatctcatc ataatgtcgt atctcctggg gtgttataca agatatcgtt gttggtgacc
     2041 tgggagagga attgagttct attaaaccgt caactatgcc ggatacatac tggattacac
     2101 agcaggcacg ccttacgaga gaacgtgccg cagtgacgag ttaattatct gaaagaattt
     2161 gcgaggctgt atcggttact cattggttta atagttttac tctcgggaga gtaatagata
     2221 tttaatccat taacggaaac cagccagttc ctttcgatgc ctgaatttga tcccatagtt
     2281 tatccggaat ggttaattca ggaacaagtt gtggattaag ggttgtttga atttcatggc
     2341 ttctacctga ttcagctaaa gtcacccatt ctggatttat caccagccct ttaccgatcg
     2401 caacaagtgg caggccagcg gaaattgctt cttgagcctg agacggtgtg cgtattttac
     2461 ctgcagcgat taccggcact ctgcctgcaa tatgatttaa aatcaattca atagttcggg
     2521 ggccatttgg cgactcaaca gggtagctat cgttaatact gaccagcgag gtatgtatat
     2581 acgaaatacc ggatgaaatg agtcgatcaa ggagtttata agtatcctct aaccgcagcc
     2641 cccctgttac agattcttca ggagatattc tgtaaccgat agcaaagggc tttgtcgcat
     2701 attcatagac tacatttttt acttcctgta atacggccag cgggaagcgc atacgtcctt
     2761 caagatctcc tccccatcgg tcattacgct ggttaaatag aggggagaag aaattctgta
     2821 gaaggaatcc gtgcgcacca tgaagttcaa tgccatcaaa acctgcttta atcgcccttt
     2881 ttgtgacatc tccgaatgct ctaatggttt cctgaatttc gttttcagtc atttccctgc
     2941 tttgtaccac tctcttcata aagtcaccag attttacgtt tgatgcgctg gcactaataa
     3001 catcgttatt tggcacaagc tccgggatgg ctttattacc agcgtggaaa atctgcaaaa
     3061 tagccggagc accgccactt ttggctgcag ctgctaactt ttcaaggctg ttaatgaaac
     3121 ggtcgtcata cgcggcaaat tcatgagtaa acccaatgcc gcttggcgtg acataggtgc
     3181 atcctgttat tactaaacca acattttgtg agcgacgttt ataaaattcg agttcttgct
     3241 cagagatggt tccatcagga ttagctgacc aggttgtcat aggagccata acgatacgat
     3301 tacgtagctt tattttttct gtcagcataa aaggagaaaa caatgatggg tgtttatttg
     3361 tcataacaat acctcaaatg tttatctgat tttaatgcgt ataatcttaa taagttgagt
     3421 tgctactttt tgatgctttt tttcctttaa tagaattctt tttgagtaat tttattattg
     3481 ttgtgtagtc aataaaggat aaatgtcagg gagataagga atctccctgt aagattatta
     3541 caaatgtgtt ttataaaaag gaattaattt ttcgagtgcc tgtttcacag gttctggctt
     3601 atcatacagg tcgtaatgtg aataaccttc aaccacgacc agctctttat gttttgaggc
     3661 agcgcggcca ataatttcgc agccgtcacg gtaggcacca aatgcgccca ctctatcgcc
     3721 caccacaacc attattggtt gagttaacag gacttctgca agatgaaatg catcccaacc
     3781 cacggcaaca gtctgatgtg agaatagcga acgatttacc ccattaggtg cacagcctcg
     3841 tggcgaacga taatattctg tggcttcaaa aaggtctatt tctgttaatc ccgcttcaag
     3901 agctgcatcc ggagaagagg ggagtaaatc atctacgcga agttttgcgc cgttagcttc
     3961 gtctgttctt tgttgcgcca tcgcttcgag tgctccgata ggattaaacg ctgtaaatcc
     4021 ctcacgcata agacgcccgt agtttgcacc cgtgacagta ccaatagctt taattcgacg
     4081 ttctgtcatt gcggcattaa tggcatatcc acctccacca caaataccca gtacaccaat
     4141 acgcccggca tcaacgtaag gtaaggtggt aaggtaatca acgacaatgc tgaagtcttt
     4201 tactcgcatt gttggatctt ccagataacg cggttctcca ccgctgcttc cctgaaaact
     4261 ggcatcaaaa gcaatgacga taaaaccagc ttttgccagg gcggcaccat acacactacc
     4321 cgatgtctgt tctttgcagc ttccaattgg atgggcgctg ataatggcag gatacttttt
     4381 ttcactatta aaatcgtcag ggaaatagat atcagcagct atatcccagt aagtatgttt
     4441 tatcgaaaca gttttcattt caagctccgg ggatttgcct aaacagattt aaatatcatt
     4501 tctattatcc gcaactggca ttcgtgttca tatcgagaac ggcgtaatag tatgacttgt
     4561 attggttatc atcaatggtc taatatctat cacagtgtta agttaaggtt tacaatgatg
     4621 aaaatagagc cttcaatttt gccttctctt gcctggtttg cgcttattgt tcgtgccggc
     4681 agtttttcca gagccgcttc tgaaatgggt attacgcggg ccgcattgtc ccagaattta
     4741 aaatctcttg aggaacgtct taacaccaag ctgatatacc gtacgacccg caatatgtcg
     4801 cttactgagg aaggacagcg tctgtatgaa gtgctggtgt cggctttggg gcaaattgat
     4861 gatgctttga aagatgttgg tgatacccaa cttgaaccta caggcctgtt gagaataaat
     4921 tcctcccgtg ttgcagcacg gatgcttgtt gagcctcata ttggcgagtt tcttactcgt
     4981 taccccaaaa caaagataga actcattatg gatgacggtt tatctaacat tattgcggac
     5041 ggctgtgatg tgggcatacg tcttgagcaa ggactggatg agcatatgac tgccgttcct
     5101 gtttctccgc ttattaaact ggttactgtg gcatcgccag attatttaaa agaacacggt
     5161 attcctgaaa cgcctcagga actgagtaac cataattgcc ttcggttgcg gcataaaagt
     5221 agcggtgcac tctctgcatg ggagttttct aatgttgtgg ggggtaatga agagttcgaa
     5281 atagaggttt caggtaagta tatttctaat gatgacgaaa gcatgatacg aatggcgctg
     5341 aatggaacgg gtatcattca acatctggat tttgcaatcg ctgaacatat caatgcgggc
     5401 aagttacagc cgattcttga ggattgggcc gtttcatttc ctggatttta tatatatgtt
     5461 tcatcacggg tgagaatgcc ctctaaagtt cgtgctttta ttgattttat ggtggagaaa
     5521 agagtaaaaa tagaaagtta atgatgtgtg tcagaatatc ggtaagaaga atggcgcaat
     5581 cgtgaggata cgtttattca taataaatct cctgaaagtt tagaattatt ttcgatacag
     5641 ccagattatc attgttacta ataatgtggt taaaataata gcatctatgc cccccttcat
     5701 gagtgtctgt gaagaggatg cttctacaag gaaatcgcca atgctcaaag aaaacttcaa
     5761 cgaactgcaa atctttcttg tggtggcaag agagcgaagt tttaccaaag cagcgggcaa
     5821 actcggcgtc tctcagtctg cacttagcca tgcaatgaag gctctggagg aaaggctcaa
     5881 tatccgcctc ctgacacgga ctacccgcag tgtggctccc acagaagcgg gggagagaat
     5941 cattgcctgc cttgaaccgc gtattgacga gcttgaacag gaactggagt cacttattca
     6001 acagaacggc acgccctctg ggaatattcg gttatctgcc ggggaacatg ccgcgcgaac
     6061 tctggtctgg ccgaagctaa aaccttttct cagagaatat cccgaaatca atgtcgaact
     6121 ggtggtggat aacggttttg tcgatattgt tgaaggccgt tttgatgccg ggatacgact
     6181 gggtgaaaat gttgataaag atatggttgc agtcaggatt ggaccagata tgcggatggc
     6241 tgtggtggga gctccggcgt attttgctgc aaaccctgcg cctcaaacgc ctcatgagct
     6301 acaaaatcat cgctgcatca acatgcgttt gccaactgcc ggtgggcttt accactggga
     6361 gttcgagcga gaagggaaac cgcttcgcgt taaggttgat gggcagctga cgtgtagtcc
     6421 gttgccagaa agaattgatg ccgccttatc agggtttggg atcgcttgtg tccctgaaga
     6481 catggttcag gagtatataa agtcaggcaa gcttattcag gtgttacagg agtggtgtcc
     6541 aacttttcct gggtattatc tttactaccc cagccgcaag cagcatccgc cagcttttgc
     6601 gctgttgatc gatgcgcttc gctacaccga ataacgggtc ctttatcctg gacccgtttg
     6661 ggtattagcg gcctacgcgt gcctgatgtt caggcgagta acgttcaccg acgattttaa
     6721 ttgtttccag cgcctcggtt atctgctgcg tatctttctg cgaaagaaca atgtcagcag
     6781 cggccaggtt ttcttccagc cggtttagtt tagtggtgcc agggatggga acaatccacg
     6841 gcttttgtgc taatagccat gccagtgcga tttgtgcaga cgttacccct ttctccgcag
     6901 ccagttcacc taacaaggtg accagttttt cattggcttc aatggcttgt gttgcgaaac
     6961 gcggtacggt gctgcggtaa tcatctttgc cgaaagtggt tcccggctta atcgcccctg
     7021 tcaggaaacc tttacctagt gggctgaagg ggacaaaacc aattcccagt tcctccagca
     7081 acggcagaat ttcctgttca ggctcacgcc accacatgga atattcgctt tgcagggcgg
     7141 tgacaggttg taccgcatgt gcacgacgaa tggtttgtgc tcctgcttca gacaggccga
     7201 aatgtttgac cttaccttct gcgatgaggt ctttcaccgt tcctgcaaca tcttcaatag
     7261 gaacatccgg atcaacacgg tgttgataca gcaggtcaat aacatctgtt ttcagacgac
     7321 gtaatgatcc ttcaacggct tcacggatat gttccggacg gctgtttaaa atctgctgct
     7381 tattatcatc gccgaaggta aaaccgaatt tagtggcaat taccacgcgg tcacgaaacg
     7441 gttttaaggc ttcgccgacc acttcttcat tcagatacgg accatacact tcggcggtat
     7501 cgaagaaggt gacaccacgt tcaaccgcag cacgaataag ttcgatagcc tgacgggtat
     7561 cggtcgccgg gccgtagccg tggcttaagc ccatgcaacc gagcccaagg gcggagactt
     7621 cgagtccgga tttacccaga taacgttttt gcattgaatg aatacctctt ttatcgcggc
     7681 ttaaacatca agtttgcggc cagtcagcca ttccaccatt gcgggatcgc ggtgagagaa
     7741 gaacgcgctg gttgcggtat ccagggcggt aatttgcagc atatcttcag ggctgagttc
     7801 aaaatcgaga atgttgatgt tctcttccat gcgttctttt cgcactgatt ttgccagtga
     7861 agcaatgcct cgttggtaga tccagcgcag aacaacctgg cccacgcttt tgccatactt
     7921 ctcgccaatt gccgtgagaa caggatgctg gaacagacca tttttccctt ccgcaaacgg
     7981 tgcccaggct tctggctgaa tgccacgact ttgcatccag ggaacggcct gtagctgctg
     8041 attgaaaggg ttaacttcaa cctggtttac cgccggaacg actttattaa aggcgataag
     8101 gtcagccagt cggtcaggat ggaagttact gacgccaata gcgcgaattt taccggcctg
     8161 ttgcagctct tccatggcac gccaggctcc gtggacatcg ccgtatggct ggtgaatcaa
     8221 atacaggtca acgtaatcaa gttgcagtcg attcagagag cgttcgaact gggctttggc
     8281 cccttcgtaa ctggtatcct gcaaccagag tttcgtggtg acaaagagtt cgttacgggc
     8341 gataccgctt tgtttcagtg cgttcccgac ctgggtttca ttttgatagg aggcggcggt
     8401 gtcaatcagt cgataaccgg tgttaatagc gtcaatgacc gctctttcgc attcagcggc
     8461 atcggtcatc tgaaatacac caaagcccag caggggcatt tcaataccgt tgttcagttt
     8521 tactttttgc atggtgttat ccttccgtta tcgttcatga gaagcataac gtaaagagaa
     8581 gagctcgatt agacggcagg atttgtcagg ggttatgaac gaaattcata aatctgtttg
     8641 agtgttgcga tgagtagtgc aagttcgata tccccgcaat ttacagtccg ataaaggaaa
     8701 atgaatatcc ataaaaaata tatcggttta tcctggcgta tatacctact tcgacgtatt
     8761 tccaatagtt gttaattaaa ggcaggccat tattattcac tctgaataat gaattattca
     8821 ctgtccgcag agtaaaaata taacttaggt atctatttaa taacttgcac aaaaagctaa
     8881 atttttaccc ataaataaaa atataatccc gcgcccaacc acctgatgag tggctacaga
     8941 cactggatat attaggtggc ggtgtacttt cttacataaa ggtatttcct tttctgcgga
     9001 aaaggaaatc ggggatttcc cggtttttct gacaagcaga cgccactatt tgtgtctgcc
     9061 tatgttcgtt aattcgttca tcaggaaatt atctcaatgt cacgttataa aacaggtcat
     9121 aaacaaccac gatttcgtta ttcagttctg gcccgctgcg tggcgtgggc aaatatctct
     9181 gttcaggttc tttttccact cgctgtcacc tttaccccag taatggcggc acgtgcgcag
     9241 catgcggttc agccacggtt gagcatggga aatactacgg taactggtga taataacgtg
     9301 gagaaaaatg tcgcgtcgtt tgccgcaaat gccgggacat ttttaagcag tcagccagat
     9361 agcgatgcga cacgtaattt tattaccgga atagccaccg ctaaagctaa ccaggaaata
     9421 caggagtggc tcggaaaata tggtacagcg cgcgtcaaac tgaatgtcga taaagatttc
     9481 tcgctgaagg attcttcgct ggaaatgctt tatcctattt atgatacgcc gacaaatatg
     9541 ttgttcactc agggggcaat acatcgtacc gacgatcgta ctcagtcaaa tattggtttt
     9601 ggctggcgtc atttttcagg aaatgactgg atggcggggg tgaatacttt tatcgatcat
     9661 gatttatccc gtagtcatac ccgcattggt gttggtgcgg aatactggcg tgattatttg
     9721 aaactgagcg ccaatggtta tatccgggct tctggctgga aaaaatcacc ggatgttgag
     9781 gattatcagg aacgcccggc gaatggctgg gatattcgtg ctgagggcta tttacctgcc
     9841 tggccgcagc ttggcgcaag cctgatgtat gaacagtatt atggcgatga agtcgggctg
     9901 tttggtaaag ataaacgcca gaaagatcca catgcgatta ccgctgaagt gaattacacg
     9961 ccagtgcctc ttctgaccct gagtgccggg cataagcagg gcaagagtgg tgagaatgac
    10021 actcgctttg gcctggaagt taattatcgg attggcgaac ctctggaaaa acaactcgat
    10081 acagacagca ttcgcgagcg tcgaatgctg gcaggcagcc gctatgacct ggttgagcgt
    10141 aataacaaca tcgttcttga gtaccgcaaa tctgaagtga ttcgtattgc tctgcctgag
    10201 cgtattgaag gcaagggcgg tcagacactt tccctggggc ttgtggtcag caaagcaact
    10261 cacggactga aaaatgtgca atgggaagcg ccgtcattac tggctgaagg tggcaaaatt
    10321 accggtcagg gtagtcagtg gcaagtaacg ctcccggctt accgtccagg caaagacaat
    10381 tattatgcga tttcagcagt tgcctacgat aacaaaggta atgcctcgaa acgtgtgcag
    10441 acagaagtgg ttattaccgg agctggtatg agcgccgatc gcacggcgtt aacgcttgac
    10501 ggtcagagcc gtattcaaat gcttgctaac ggtaatgagc aaaagccgct ggtgctgtct
    10561 ctgcgcgacg ccgagggcca gccagtcacg ggcatgaagg agcagatcaa gactgaacta
    10621 accttcaaac cggctggaaa tattgtgact cgttccctga aggccactaa atcacaggca
    10681 aagccaacac tgggtgagtt caccgaaact gaagcagggg tgtatcagtc tgtctttacg
    10741 accggaacgc agtcaggtga ggcaacgatt actgttagcg ttgatggcat gagcaaaacc
    10801 gtcactgcag aactgcgggc cacgatgatg gatgtggcaa actccaccct gagcgctaac
    10861 gagccgtcag gtgacgtggt tgctgatggt cagcaagcct acacgctgac gctgactgcg
    10921 gtggactccg agggtaatcc ggtgacggga gaagccagcc gcctgcgact tgttccgcaa
    10981 gacactaatg gtgttaccgt tggtgccatt tcggaaataa aaccaggggt ttacagcgcc
    11041 acggtttctt cgacccgtgc cggaaacgtt gttttgcgcg ccttcagcga gcagtatcag
    11101 ctgggcacat tacaacaaac gctgaagttt gttgccgggc cgcttgatgc agcacattcg
    11161 tccatcaccc tgaatcctga taaaccggtg gtcggcggta cgcttacggc aatctggtcg
    11221 acaaaagatg ccaatgacaa ccctgtgacc agcctcacgc cggaagcgcc gtcattagcg
    11281 ggtgccgctg ctgttggttc tacggcatct ggctggacaa ataatggtga tgggacgtgg
    11341 actgcgcaga ttactctcgg ctctacggcg ggtgaattag aagttatgcc gaagctaaat
    11401 ggacaggatg cggcagcaaa tgcggcaaaa gtaaccgtgg tggctgatgc gttatcttca
    11461 aaccagtcga aagtctctgt cgcggaagat cacgtaaaag ccggcgaaag cacaaccgtg
    11521 acgttggtgg cgaaagatgc acatggcaac gctatcagtg gtctttcgtt gtcggcaagt
    11581 ttgacgggga ccgcctctga aggggcgacc gtttccagtt ggaccgaaaa aggtgacggt
    11641 tcctatgttg ctacgttaac tacaggcgga aagacgggcg agcttcgtgt catgccgctc
    11701 ttcaacggcc agccagcagc caccgaagcc gcgcagctga ctgttattgc cggagagatg
    11761 tcatcagcga attctacgct tgttgcggac aataaaactc caacggttaa aacgacgacg
    11821 gaactcacct tcaccgtgaa ggatgcgtac gggaatccgg tcaccgggct gaagccagat
    11881 gcaccagtgt ttagcggtgc cgccagcacg gggagtgagc gtccttcagc aggaaactgg
    11941 acagagaaag gtaatggggt ctacgtggcg accttaacgc tgggatctgc cgcgggtcag
    12001 ttgtctgtga tgccgcgagt gaacggccaa aatgccgttg ctcagccact ggtgctgaat
    12061 gttgcaggcg acgcttcccg ggcagtgatc agtgatatgg cggtgaaggt gaataaccaa
    12121 ctggcaaatg gacagtctgc taaccaggta accctgaccg tcgtggacag ctatggtaac
    12181 ccgttgcagg ggcaagaagt tacgctgact ttaccgcagg gtgtgactag caagacgggg
    12241 aatacagtaa caaccaatgc ggcaggtaaa gcggatattg agttgatatc aaccgttgca
    12301 ggggaacttg aaatcgcggc cgcggtgaaa aactctcaga agacggtcac ggtgaaattc
    12361 aacgcggatg ccagcaccgg tcaggcaaac ctgcaggtag acaccgccgt tcaaaaagtg
    12421 gcaaacggca aagatgcctt tacgctgacg gcgaccgttg aggataaaaa cggtaaccct
    12481 gttccaggca ctctggtgac ctttaatctg ccccggggtg tcaagccgct tactggcgat
    12541 aatgtctggg tgaaagccaa cgatgagggg aaagcagagt tgcaggtggt ttccgtgact
    12601 gccggaacct atgagatcac ggcatcggca ggaaatgacc agccttcgga tgcgcagact
    12661 ataacgtttg tggccgataa ggcgaccgca accgtctccg gtattgaggt gattggcaac
    12721 tatgcactgg cggatggcaa agccaaacag acgtataaag ttacggtgac tgatgccaat
    12781 aacaacctgt tgaaagatag cgaggtgacg ctgactgcta gcccggcaaa tttagctctg
    12841 gatcccgatg ggacggcgac aacgaatgag caaggacagg ctattttcac cgccacgacc
    12901 agtgtcgcag cgacatatac actcacggcg aaagtggaac aggccgacgg tcaggaatcg
    12961 acgaaaactg ccgaatctaa attcgtcgcg gatgataaaa acgcggagct cgctgcatct
    13021 ccagagcgtg tagattctct ggtggcggac gggaagacta ctgcaacact gacggttact
    13081 ctgatgtcgg gtgtcaaccc cgtaggagga accatgtggg tcgacattga ggctccggaa
    13141 ggggtgacag aggcggatta tcagttcctg ccgtcgaaaa atgaccattt cgcgagcggg
    13201 aaaatcacgc gtacatttag taccaacaag ccaggtacat acacattcac attcaactct
    13261 ttgacatatg gagggtatga aatgaaacca gtgactgtga caattaacgc cgttcctgca
    13321 gatactgaag gtgctgagga gaaataaaaa cgacgatgta ataaataata atggcgttga
    13381 catgcgcagt gccattattt actgaatacc taccctggta ccgattagac ggtggcaggg
    13441 tagggtaaat cttatgtgat ggatttacag gctaaaccgg gcgaacgggg ataaaaattt
    13501 cgcaaacaat gttattgcta tcaatttcga cgaagttttc attgtaataa aatcgctcaa
    13561 tatcactgcc gttcactctg gctaaccccc tggcaggtaa ttccatcaga tagaggtttc
    13621 gcgcccattc agggtagtgc tcccactcac cttcatagcg gaaagaggca tacagtcctc
    13681 ggttaattgt ccactctttc gacatctctg aatcggtttg tttgcatacc gtaccggcaa
    13741 aagtttcaac tgtgagcaaa tcgaccgatt ttgttgacgg ttcgaatgtg gagacgaaat
    13801 agatatccgt cttaaaattc ttcatacatc tggctaaatg atgacgtcgc agtttgactt
    13861 cttcatccgg tgatttattc gatacttcaa tatcatattt aaatgaattg ccaaaaatag
    13921 gtgtctcagg aaagttgact aatctacact ccgtcttttg ctgttgacgt attaaaaaag
    13981 aagggaagat atttgcgaga tcccaataat cacggtggcg gtattcacgg ggagagcagc
    14041 cgaataactt tttaaattcg cggctgaatg actgctgtga gtcaaaatgt aaagagagtg
    14101 caatatcaag catagatttc gcggtgagtc ggacaagaat ggcggcacga caaagccttc
    14161 gtttgcgaat gtattctccc aaaggcacat gcatgaaatt tcggaacaga agctggatgt
    14221 tgcgtctgct gtagccagat ttctgtgcga tatcttcgat tgaaataggg tgctcaagat
    14281 tgcactcaat ccactccagg agctgctgta gaatcttctg cctgatcatc acattctccg
    14341 ttaattcttt gtaatatcgc acattttcgt tatgtcctgg gccttaagtg tatcaaaaac
    14401 agcaagtccc aaggcaaaag cactgatagc gtctgcttca ttgccccaaa aatagagagg
    14461 tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc cacatactaa
    14521 tttgaggtta cgttttaacg tagactcatt gttcatacct aatggaggga ctgacagtgg
    14581 aattttccgt attaagtaac aatctgaaaa tgccgatgat gggatttggt gtttttcagg
    14641 ttaccgataa agacgtgtgc aaacagtcag tgttgaatgc tatccgcacg ggttatcgac
    14701 tcatcgacac tgcagcggta tacggtaatg aagatgccgt cggtgaagcc gttcgtgaag
    14761 ctatatctga aggtttatgt acccgagatg agttatttat aacgtctaaa ttgtgggtgc
    14821 aggatatgtt gaatcaggat acggcaaaag caggtattga agcatcatta aaaaaatcgg
    14881 gactagaata cttcgacctc tatttattgc accaggctat gcgcgattat ttcagtgcgt
    14941 ggcgtgcact cgaagatgcc tatgaagagg gcaaattaaa agcaattggg gtttccaatt
    15001 tctatcctca tgttctggcg aacttttgtg aaacggtaag agttaaaccg atggtcaacc
    15061 aggtcgagtt gcatccctat tttgcccaac cagaggcgct ggcaaccatg aagtattata
    15121 acgtgcagcc tgaagcatgg gctccgttag gtggtggacg acataaaccc tttgaaaata
    15181 atctgcttca gagtattgca gatgcccatc aaaaatcgat ttctcaagtc attctgcgtt
    15241 ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag cgtatcgaag
    15301 aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcacaa attagttcgc
    15361 ttgatttggg ttatgttggg gagtcggtaa aacattttaa tcctgaattt gttcgtggtt
    15421 gtcttgctgt aaaaatacat gattgatatt aatcatgtat tttacctgag acgacaagaa
    15481 tcttttaaca ggggagtgat attgatcttc actctgtcat atctccggta atatggcgtc
    15541 aggcttcatc atcagaaagg gggcctgacg cctgaaaaag tgaacaacag acagtgttcg
    15601 gattatcaat attcagtttt taacgttgaa ctggattcat tactgcgttg tttaagaatt
    15661 tcccgcgccg aatctgccat tatcatgaca gcacctgcca gcatcagagt atctttcaat
    15721 accaggcgac cagcaccgga taaataaggg aaaccatgat gagcgtcacc caatgcgggt
    15781 acccatgcct ccggggtggt gattaaaaat gagagtgtta ccaacggtgt ggtaaatgcc
    15841 atcagccctc ccaataaacc taaccagcga ttgacaggat tagccaaaac cagcagagca
    15901 ataatcacct ccacgacgcc aagaccgttg gaaaaaccat aggtattatt ggccgattgc
    15961 catgcccttg cttctggttt gtattcgcct tcgtgagtca gatactgttt atagtcttcc
    16021 gggtgttcat aaaagaacga cattagtgga ctgtttgcga cgaatggtgt aatgctgtct
    16081 gcctcgtaag ggacaaactt taataaccca atccacataa aaacaatcgc aatactcaga
    16141 cgaatcaatg tcaggccaat tttatctccc cgacttaaca gatgcaggta tttttccatt
    16201 gtgaacatcc ttatttatac aatactgctg ttgcggttga attattatca attcgcatcg
    16261 atgtgacttt ccaggatgaa gcattatatt ttctggctat atcagcaatg gcatcctcca
    16321 gaactgtcgg tgatgctgat actgacgaga cgctgatatg tttcagaatt attttatcat
    16381 ctgcattggt ggaaaatgcc ataacgccag ataatagtgt tgcaaataaa acggattttt
    16441 taaacatata aaataccatc aatgaagtga ttaagagttt atagagttac agagaattaa
    16501 taagattctt ttctctgaga cgccagaata tttgttctgg cgtctgattt tgagtttatt
    16561 tgactaatga aaatag
//
LOCUS       DAOTRK010000106        16295 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.106, whole genome shotgun sequence.
ACCESSION   DAOTRK010000106 DAOTRK010000000
VERSION     DAOTRK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.106"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1208)
                     /locus_tag="QIE41_003288"
     CDS             complement(<1..1208)
                     /locus_tag="QIE41_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532141.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQHQLQQINACHTNTTYTNADYSKIVAQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKNSECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFNAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFSHKEHPTPKLYKDEQYLDKQIEG
                     LFSTLLTINDNFLRAKAEIASTIIKFLEARITNLSYNDILKYQQEFQKQCYKQVKAFT
                     TLSRYNKIQTWAEMSEYQFEVFQYETLNPKKMSRTPYLKRSLPDEKTINYGVEIEIPS
                     GKRIRLSNHY"
     gene            complement(1788..2789)
                     /locus_tag="QIE41_003289"
     CDS             complement(1788..2789)
                     /locus_tag="QIE41_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDS3532142.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQMLF"
     gene            2897..3193
                     /locus_tag="QIE41_003290"
     CDS             2897..3193
                     /locus_tag="QIE41_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3532143.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(3316..3741)
                     /gene="arsC"
                     /locus_tag="QIE41_003291"
     CDS             complement(3316..3741)
                     /gene="arsC"
                     /locus_tag="QIE41_003291"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDS3532144.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(3754..5043)
                     /gene="arsB"
                     /locus_tag="QIE41_003292"
     CDS             complement(3754..5043)
                     /gene="arsB"
                     /locus_tag="QIE41_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDS3532145.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALLTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYALVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLAENGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(5098..5451)
                     /gene="arsR"
                     /locus_tag="QIE41_003293"
     CDS             complement(5098..5451)
                     /gene="arsR"
                     /locus_tag="QIE41_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDS3532146.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSEDSKNICS"
     gene            6194..6277
                     /locus_tag="QIE41_003294"
     CDS             6194..6277
                     /locus_tag="QIE41_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDS3532147.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(6331..7683)
                     /gene="gorA"
                     /locus_tag="QIE41_003295"
     CDS             complement(6331..7683)
                     /gene="gorA"
                     /locus_tag="QIE41_003295"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDS3532148.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7755..8597)
                     /gene="rlmJ"
                     /locus_tag="QIE41_003296"
     CDS             complement(7755..8597)
                     /gene="rlmJ"
                     /locus_tag="QIE41_003296"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDS3532149.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8800..10842
                     /gene="prlC"
                     /locus_tag="QIE41_003297"
     CDS             8800..10842
                     /gene="prlC"
                     /locus_tag="QIE41_003297"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDS3532150.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10850..11602
                     /gene="rsmJ"
                     /locus_tag="QIE41_003298"
     CDS             10850..11602
                     /gene="rsmJ"
                     /locus_tag="QIE41_003298"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDS3532151.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11651..13120)
                     /gene="dtpB"
                     /locus_tag="QIE41_003299"
     CDS             complement(11651..13120)
                     /gene="dtpB"
                     /locus_tag="QIE41_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDS3532152.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(13436..13870)
                     /gene="uspA"
                     /locus_tag="QIE41_003300"
     CDS             complement(13436..13870)
                     /gene="uspA"
                     /locus_tag="QIE41_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDS3532153.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            14261..14596
                     /gene="uspB"
                     /locus_tag="QIE41_003301"
     CDS             14261..14596
                     /gene="uspB"
                     /locus_tag="QIE41_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDS3532154.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14667..16166)
                     /gene="pitA"
                     /locus_tag="QIE41_003302"
     CDS             complement(14667..16166)
                     /gene="pitA"
                     /locus_tag="QIE41_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDS3532155.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT         4210 a         4012 c         4113 g         3960 t
ORIGIN      
        1 tgataatgat ttgataagcg aattcttttg ccggatggta tttctatttc caccccataa
       61 tttatagttt tttcatcagg tagcgatctc tttaaataag gcgtgcgtga catttttttt
      121 gggttaagtg tctcgtattg aaatacttcg aattgatatt cagacatttc tgcccatgtt
      181 tgaattttat tgtatcggga gagtgtcgta aaggctttaa cttgcttata acattgtttt
      241 tggaattctt gctgatattt taatatgtca ttgtatgaaa gattggttat cctggcctct
      301 aagaatttaa ttattgtcga agcaatttcc gcttttgctc gtaaaaagtt gtcattaatg
      361 gttaataatg ttgaaaataa accttcgatt tgtttgtcaa gatattgttc gtctttatat
      421 aattttgggg tagggtgttc tttatgtgaa aaaatagtgt cacctaaatt tgcgtcagtg
      481 aaatttatat taagtagatc ttcgaaataa aaccttgaca aggttagaac gaacccggac
      541 aggttctgtc ctgtcatatt gaaatcaata aagttggttc cggcaaagac tttcatcctg
      601 tttttatttt cactgtgttc aatatataac ttcgtcattt tttgatacag gctttgaata
      661 tcttttatat tagtcggtaa gtcagctcgt tgttcagcat taaaattagt atctgaaaac
      721 atggtttgga cactgaatct tactggtatt ccctcttcta cgctaatttt agttatgatt
      781 gccttgtcgg gtttaggatt agcgacaaca atgattcgac attcagaatt tttattcttt
      841 tcgtatctaa tgttattaat ctgattaagt attgattgtg tctgttgcgt aagtggtgtc
      901 gaacttatcg tttcaatgtt attaataaga cttacaagtt gagccacaat ttttgagtag
      961 tctgcatttg tatatgtagt gttcgtgtgg cacgcattaa tttgttgaag ttggtgttgc
     1021 gctaaatcat attgatcaga ggtatatcct tctccagaga atgtggaatt aatgggttgt
     1081 tgttcttccc aattatcgca aggccacttc ggtgtatctg gcgttttagc attatcggta
     1141 tttgttgtgc agttataaat atcgccgtga tatgtattta ttatacctgg ggtaaagtca
     1201 attgacataa aattccttat tcatcatttg ttttatgcta agatgtttaa attggataat
     1261 tagaacagga atggattttc ttctacacac aataaatgta atgcgttatc tcttcctgat
     1321 ataattacct ttgatttccc ttttatctcc acccggtaat atttgtgtat aaaaaaaggg
     1381 cagtgatagt atcgtaaagg aaaacgttaa caactatcaa aaagcgctca ttttaaaaag
     1441 ttatgatgtt caccgatagt gtgtgaagtg atatttttgt gatgttttag atgtgtaata
     1501 atgaaccagg ttaaagtgta tttaaaaatg tgttgtattg gcgactgaaa tctgcccttt
     1561 aaaaggggga tagcactgaa acaaagagac attgcttctc cgataaaatg tcagagaagc
     1621 atgattgcgt ggtgattgtc gattgatgac ttactgacgg atccgtcatc attctcaaag
     1681 cattagttaa gttatttgtg atttttaagg attatcctat ctatgatttt ctactcagaa
     1741 ggataaggca ctcacaatgc tccttatagt ttactgctgg catctattta aaatagcatt
     1801 tgagtcaaat agcccatcaa gatagccata cctaagataa ccgtcaggaa tgcagctatc
     1861 atcggcattc tgaacattga tttaagtaat atcacttccg tcaggcttgc tccggcactg
     1921 ccgatgatta atgccattaa tgctcccagc cccattcctt ttgtcatcag aacagatgcc
     1981 agagggataa ccgcctctgc ccggatatac agcggaattc cgacaacggc gctcaatggg
     2041 atggcgaagg gattatctgc ccctgcatga gcggcaatcc actctgaggg aataaagcca
     2101 taaataaaag agcctattaa tacccctaac aaaagatatg gcagtacatc tttaaactgt
     2161 tgcaacgcat ctttggtggc tagttttatg ggattaatac ttaccacagc tttggtactg
     2221 caacagttta ctacaggttt ctcaatggct ttagccgctg gactacagca actcactttt
     2281 attggcgtat atgtcgtccc cggcgaagtt ttggctggag cacaacaatt tgctgatgag
     2341 cttttactgg caatgatatg acgttcaaat cccagggaat ccaggataat actggcaagt
     2401 acggagacgc cggcggcgat aatcgcgtac aacaaggtaa ctttccagcc aaaggtcacc
     2461 cacattaacc cgacgataat gggattaagt aatggggaaa caaataaaaa agtgagagtc
     2521 ggaccaaacc cggctttcgc tgataacaat ccacgtaaca tggggattgt cgagcaacta
     2581 cagaacgggg taacggctcc caacagagca gccaggagat agcctttccc ttttcttgcc
     2641 cccatcatct gctggatttt atggtctggc accttttgtc ttatcaggct gacaccggca
     2701 ctaatcacta taaacagcaa agaaagctcg acggcgagaa acacaaacat ctctgcggca
     2761 tcttgcagca tcgcaagcca actactcata cgtaacctca ttgaatgaat ccatatttcc
     2821 agaataatcg aaatgaaatt cagatcaaga tatttctggt attatcgaaa tatcaaccag
     2881 acaaaggggg tgtcatatgc agttggaaga agttgctaaa gcgctgaaag agctaggtca
     2941 cccgacccgt ttgttcatat tcaaacacct ggtcaaagcg ggggaacaag gtttgccggt
     3001 cggtgagttg caaaagcagc ttgggatccc cggctccaca ctcagtcacc atatttcagc
     3061 actggtatcg gtagggctgg tcaaacaaaa ccgtgagagt cgaacgctga tgtgtgtttc
     3121 gcaatacgca atgctggaag cgataattga attcttacgt gaggagtgct gcgtaaatag
     3181 caaaattgcc tgatgcgcta cgcgctacag atttcatgcc atgcacggtt ttgtaggtcg
     3241 gataagacgc gttagcgtcg catccgacat aaacaaagcg cactttacca actatgggca
     3301 gtggatatcg ccgctttatt tcaagcgttt acccgcttca tcaaccactt tctcgccatc
     3361 ttccttggtg aacgcgcctt gttgcgcatc tggcagaatt tccagcacga cttctgaagg
     3421 gcggcacagg cgagttccca gcggcgtcac cacaatcggg cgattaatca gaatcgggtg
     3481 ctgaagcata aagtcgatta accgatcgtc agtaaattta tcttctgcaa ggcccagttc
     3541 ctcatacggc tcgacgtttt tacgcagcag cgcgcgtacg gaaatcccca tatcggcaat
     3601 gagtttgacc agttcatcac gcgtcggtgg ggtttccaga taatagacaa tggtcggttc
     3661 tgtgccgctg ttgcggatca tctccagcgt atttcgcgac gtgccgcagg ctgggttgtg
     3721 ataaatggta atgttgctca tatcaatatc tcattacaaa gtgaaagaga gacgtagcgc
     3781 cagcgcagcc agcgtcacaa aaagcaccgg cagggtcatg ataatccctg tacggaagta
     3841 atatccccag ctgatagtca tattcttctg cgaaagtacg tgcagccaga gcagcgtcgc
     3901 caggctacca attggggtaa ttttcggccc caaatcgcag ccaatcacgt tggcataaac
     3961 catcgcttct ttgataacgc cagatgccgt gctgccatca atggacaacg cgccaaccag
     4021 taccgtcggc atattgttca taatagaaga gaggaaggcg gtgaggaatc cggtgccgag
     4081 cgtcgcggcc cacaggccgt tttccgccag cacgttgagt acgccagaaa gatattccgt
     4141 tagtccggca ttgcgcaggc cataaaccac cagatacatg ccgagcgaga agatgacaat
     4201 ctgccagggc gcaccgcgta gcaccttacc cgtattaatt gcatgaccgc gtttagcgac
     4261 ggcaaataat atcagcgcgc ccactgccgc aatggcgctg accggaatgc cgagcggttc
     4321 cagaacgaaa aatcccacca gcagaagcag taaaacaacc cagccagttt tgaacgtagc
     4381 aggatcttta atcgcttctg cgggagattt cagcagcgcc atgtcgtagt tctgcggaat
     4441 atctttgcgg aaatagagat gcaacatcac cagcgtggca acaatcgcgg cgatatccac
     4501 cggcaccatc accaaggcat attcgcgaaa gccgaggcca aagaaatctg cggaaacgat
     4561 attcaccagg ttggagacaa taagcggcag gctggcggta tcggcaatga acccggccgc
     4621 catcacgaac gcaagcgtcg tgcctttact gaaccctaaa gccagcagca tggcgatgac
     4681 aatcggtgtc aaaataagcg ccgcgccatc attggcaaac agagctgcaa cggcagcacc
     4741 gagcaggaca atccaggtaa acagcaagcg accgcgtcca ttgccccagc gtgagacgtg
     4801 cagcgccgcc cattcaaaaa agccggactc atccagcagc aggctgatga taatgacggc
     4861 aataaacgcc gccgtcgcgt tccagacgat attccacacc accggaatat cacccggatg
     4921 aaccacgccc gtaagcaacg ccagtaccgc gccgagcgtt gcactccagc cgatgcctaa
     4981 acctttcggc tgccagataa ccaatacgat ggtcaggaca aaaatagcgc ctgccagtaa
     5041 cataatgcct cccggataaa acacatctga aaattcatat gtgtttagat aaatttttta
     5101 actacaaatg ttcttactgt cttcggaaca attttgtcga gccaggttgc ggacaatcgc
     5161 ctgaaccttt tcctgttcac atcgccaggc ctcatcaata attttcgcgg cccatgatgg
     5221 aatatgcggt gataagcggt aatgaaccca cttaccctgc ttgcgatcca gcaatagccc
     5281 actttcacgc agcaatgcca ggtggcggga tatcttgggc tgcgactgat cgagggcagt
     5341 acagagatcg cagacgcata attctcccag ctcgctgagc agtaaaacga tgcccagacg
     5401 ggtttcatca gcaagaattt tgaacagttg gatgggtaac agaaatgaca tattgcgctc
     5461 ctgattgttg caggtagtgt ctctcttcga agcggataag tcaaaaacat atatgactta
     5521 acgaatgtgt aagcgcagaa gaaggtaata ggtgtgaatt ttgagttggg tattcatttg
     5581 aaaggaggtc tgaatcagga cgtaaaaaaa ccgactttgc gtcggttttt tactttccag
     5641 ccctgagttg gtggctctgg ccggagtgag aatgcccaca tctaacggtt caatactatt
     5701 agccagtggc taacattgca agcgttagcg atttcttcag ctggcgaagg gacagtttgc
     5761 tatacatgtt ggtgaaagag gatttatata ctttcatcgg gtcgctgccg taccaggcct
     5821 tgtccaaatc cggacgtaaa aaaaccgact tcgcgtcggt tttttacttt gcagcccaga
     5881 gttggtggct ctggctggag tgagaatgcc atcatctaac gcatcaatgc taataccata
     5941 ctgaaactat tgcaaggaca tgctggtttt ataacctgta tgtactgtat gattatccag
     6001 ttagttctga ggcattttca ctctggtaat gcgcataaac gctttcaaag tcctggtcag
     6061 aagtacgggt ggtgccgtta actgatgctc tggtcggagt gagaatgccc acatctaaca
     6121 atggggcatg cgccgtgaca ggcagtggat gagtaagcgg atgcattctc actccatcgc
     6181 atggagaaaa cgggtgattg ataaagcaat catcgttctt ggggcgttaa ttgcgctact
     6241 ggaactgatc cgcttcctgc ttcagcttct gaactgatag cggaaacgta gttaagggca
     6301 aagagcacac cactctttgc cctttaacat ttaacgcatg gtcacgaact cttctgccgc
     6361 cgtcgggtga atggcgacgg tattgtcgaa gtcttttttg gtcgccccca tcttcagcgc
     6421 caccgcgaag ccctgcaaca tttcatccat accaaaacca atgccgtgaa ttccgacaat
     6481 cttctcttcc ggtccaacgc ataccagttt catgcggcac ggctggcggt gagtggtgac
     6541 ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt cgccgtactg
     6601 ctcgcgcgcc tgcggttccg ttaaaccaac agtaccaatc ggcggatggc tgaagaccac
     6661 ggtcggaatg ttgctgtaat ccagatgctc atcaggcttg ttattaaaca ggcgttcaga
     6721 gagacggcga cccgctgcaa ctgccaccgg tgtcagttcc actgcccccg tgttatcgcc
     6781 cacagcgtaa ataccttcaa cgttggtatt ttgatattta tcgacgacga tatagccttt
     6841 ttcgttagtt ttaacgccag cggcttccag gttgatgttg tcattggcag gctcgcgacc
     6901 aatcgcccaa atcaggcaat ctaccgtttc actgcgacca tcttccagct ccagcgtcag
     6961 gctgccgtcg gcatttttca ccaccgcttt cgggatggcg ttggtgtgca gctgcgggcc
     7021 ttcggcgttc atcacttcga ccagcgtttc agagatcatc gggtcgaagc tgcgcagcgg
     7081 cgcatgttta cgcacaaaca gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag
     7141 ctcaacggcg atgtaacccg cgccaacaac cgccacgcgc tctggcaaag caggaagggc
     7201 gaagaagcca tcagaatcaa taccgtattc cacgcccgga atatccgggt ggctcggacg
     7261 accgccggtg gcgatcagaa tatgatcggc cgtgatggtt tcgccgttta cctccagcgt
     7321 tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt taccgagcac
     7381 gttttcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga tcaacgtttc
     7441 ccagttgaat ttatttatag tggtatcgaa accataatcc ggaccgtaca tgtggatcgc
     7501 ttcacggatt tgcgccgcgt gccacatcac tttcttcggt acgcagccga cgttcacgca
     7561 ggtgccgccc agctctttgg cttcaatcag cgcgcatttc tggccgtaca tagccgcgcg
     7621 gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat agtgtttagt
     7681 catgacagag tgtccttatc gttgattacc gcgattgtag cgcgagacgt aataggtgcc
     7741 agcaatggct gcaattactc cggcacgatc cagcttacgg tggcgtgccc ggtgcctgcc
     7801 ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgctgttc cagcttccac
     7861 ggcgggttaa tcacaatcat gccggaagcg gtcatgccac ggcgatcgct gtctggcagt
     7921 accgccagtt caatttgcag aattttgcga atgccggtcg cttccaggtc gtggatcatg
     7981 cgcttaattt gctggcgcaa caccactgga taccacagtg cgtaagtgcc agtggcgaaa
     8041 cgtttatagc cttctgctat cccgctgacc accgcctgat agtcggtttt catttcatac
     8101 ggcgggtcga taaggattaa accacggcgg gaaaccggcg gcagtttggc cttaagctgc
     8161 tggaaaccat cggctttttc gacgcgcgca cgattgtctt tctgaaattc agaacgtagc
     8221 aacgggtaat cgctcgggtg cagttcggtc agttgcaggc tgtcctgttc acgcagtaac
     8281 tgacgagcaa tcaacggcga accggggtag taacgcaact gaccgctacg gttgaagtgt
     8341 tttaccacat tgatgtacgc ctccagctct gcaggcaaat cgtcctgctg ccagatacgg
     8401 gcgatacctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc
     8461 ccggcccctg cgtgggtatc gagatagaga aacggtttat ctttctcttt cagcgactcg
     8521 atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg
     8581 tggcgataac tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg
     8641 gcgcacaaaa gcctatcagg acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac
     8701 gtttctcatt gaaattcact acacttaacc ccatgctaca aacattatgt aaagcgcctg
     8761 ttgagcgctt ccttaacctc tttaaccagg actgcgctaa tgacgaatcc gttactgact
     8821 ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc agccgtgact
     8881 aaggcgctga acgactgccg cgaaaatgtg gagcgcgtag tagcgcaagg ggcaccgtac
     8941 acctgggaaa atctctgcca gccgttggcg gaagtggacg atgtgctggg gcgtatcttc
     9001 tccccggtca gccacctgaa ctcggtgaaa aatagcccgg aactgcgtga agcgtacgaa
     9061 caaaccctgc cgctgctgtc agaatacagc acctgggtag gacaacatga agggctgtat
     9121 aaagcatatc gcgacctgcg cgatggcgat cattacgcca cgctgaacac ggcgcagaag
     9181 aaagcggttg ataacgcact gcgcgacttt gaactctctg gcatcggtct gccgaaagag
     9241 aaacagcagc gttatggtga gattgccact cgtctttccg agctgggcaa ccagtacagc
     9301 aacaacgtcc tcgatgcgac aatgggctgg accaaactcg ttaccgacga agcggagctg
     9361 gcggggatgc cagaaagcgc gctggctgcg gcaaaagccc aggctgaagc gaaagaactt
     9421 gaaggctacc tgctgacgct ggatatccca agctacctgc cggtaatgac ctactgcgac
     9481 aaccaggctc tgcgcgaaga gatgtatcgc gcttacagca cccgcgcctc cgatcaaggt
     9541 ccgaacgccg gtaagtggga caacagcaag gtgatggaag agatcctcgc gctgcgtcac
     9601 gaactggcgc aactgctggg ctttgaaaac tacgccttta aatcccttgc tactaaaatg
     9661 gcagaaaacc cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg cgcgcgtcca
     9721 caaggcgaaa aagagctggc gcaactgcgt gccttcgcta aagccgaatt tggcgtggat
     9781 gagttgcagc cgtgggatat cgcgtactac agcgaaaaac aaaaacagca cctctacagc
     9841 atcagtgacg aacagctgcg tccgtacttc ccggaaaaca aagcggttaa cggcttgttt
     9901 gaagtggtga aacgtattta cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg
     9961 catccggatg tacgtttctt cgaactgtat gacgagaaca acgaactgcg cggcagcttc
    10021 tacctcgacc tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga tgactgtgta
    10081 ggccagatgc gtaaagccga cggttcgctg caaaaaccgg tcgcgtatct gacctgtaac
    10141 ttcaaccgcc cggtaaatgg taaaccggcg ctgtttaccc atgacgaagt gatcaccctg
    10201 ttccacgagt tcggtcacgg cctgcaccat atgctgaccc gcattgaaac tgctggtgta
    10261 tctggtatca gcggggtgcc gtgggatgcg gtcgaactgc cgagccagtt tatggaaaac
    10321 tggtgctggg agcctgaggc gctggcattt atctccggtc actatgaaac cggcgaaccg
    10381 ctgccgaagg agctgctgga taaaatgctg gcggctaaga actaccaggc ggcgctgttt
    10441 attctgcgcc agctggagtt cggcctgttt gatttccgcc tccatgccga gttccgcccg
    10501 gatcagggag cgaaaatcct cgaaactctg gcagaaatca agaaactggt tgccgtagta
    10561 ccgtctccat cctggggccg tttcccgcac gctttcagcc atattttcgc cggtggttat
    10621 gccgcaggtt actacagcta cctgtgggcc gacgtgctgg cggcagatgc cttctcgcgc
    10681 tttgaggaag agggcatttt caaccgtgaa accggacagt cgttcctcga caacattctg
    10741 agccgtggcg gttcagaaga gccgatggat ctgttcaaac gcttccgtgg tcgtgaaccg
    10801 cagctggatg cgatgctgga gcattacggc attaagggct gatcattcag tgaaaatctg
    10861 cttaattgat gaaacaggca ccggagacgg tgccttatct gttctggcgg cccgctgggg
    10921 gctggagcac gatgaagaca acctgatggc gctggtatta acgccggagc atctggaact
    10981 gcgcaagcgt gatgagccaa aacttggcgg catcttcgtt gattttgttg gcggagcgat
    11041 ggcgcaccga cgcaaattcg gtggtggtcg cggtgaggcg gtggcgaaag cggtgggcat
    11101 taaaggcgat tatttgccgg atgtcgtcga tgctaccgca gggctggggc gcgatgcctt
    11161 tgtgctggct tcagtcggct gccgcgtgcg gatgctggag cgtaatccgg tggttgctgc
    11221 attgctcgac gacggcctgg cgcgtggtta tgcggatgcg gaaatcggcg gctggttgca
    11281 ggagcggttg cagttaattc acgcctccag cctgacggcg ctgaccgata ttaccccacg
    11341 cccgcaggtg gtctatctcg atccgatgtt cccgcataag cagaaaagcg ctctggtgaa
    11401 aaaagagatg cgtgtctttc agtcgctggt ggggccggat cttgatgccg atggattact
    11461 agagcctgca cgcttgctgg cgaccaaacg cgtggtggta aagcgcccgg actacgcgcc
    11521 gccgctggcg aatgtcgcca cgccaaacgc ggtagtgacc aaagggcatc gctttgatat
    11581 ttatgccgga acgccggtgt aaaagtaaag cagggtagcg ttatcgctac cctgctttct
    11641 gcaagaataa ttaatggctt tcgggcgtcg caatcattcg tttcagccac ggcaccatca
    11701 gcagcatcac caccgcaacg cccagcgtaa ccaggccaat cttaccaaac acgttggtat
    11761 agacgggcaa cgtctcaagc ggatcggtaa tgttgtctgg tactgcggta aatgttgcca
    11821 catagccgcc cagcaagaac gcggcagcct gcgtcaggaa ccacatcccg agaataaagc
    11881 ccatcaaatg ctgcggcacc agggcagcaa tcatcgccag acccagggcg ctaataaaca
    11941 attcacccag gctctggaat aagtaaacca gcacaataaa ccacggcgat gtcagtcctt
    12001 gggcatccgc aaaccacatt cccgcagctg cagccgtcaa aaagcccagt gagcacataa
    12061 acatgccgag agtaaatttc atcggcatag agaggtcttt gcctttgtta cccagatgcg
    12121 tgtaaatgcc tgccagtatt gggctggcga gtaccaccca gaacgggtta agcgcctgga
    12181 agctgaccgg gttgatggaa aagccgagaa tttcatgatg cacgttgttg atggcaaaga
    12241 agttcagcga cgttggcatc tgggcgtaga gaatgtaaaa caccaccgct tcgagcatca
    12301 ggacaaaggc gacaaacatt ttattgcgcc cggttttatc cagcttgaat gcctgacgaa
    12361 agaaaatgat ggtgacgacg atagagagaa caatcagcac cagattggcg acttctacgt
    12421 tgtgcatcag ccatgcacag acgaagatca tcaccacgct gccaagtaac acgtacaaca
    12481 gtttgctgaa gctcatcggc cggaagtcgg gttcagaacc aatgtctttc accattccgc
    12541 gacaggcgat gtaaaccaat agcgcgataa ttaaccccgc accgcacagg ttgtaggtga
    12601 ctgaataacc gaatctatca gcgatcacgg gggccagcga taacgctatc aacgagccga
    12661 tgttgatcga catatagaac agggtaaatg cgccatcaag ccgcggatct ttcggcggat
    12721 agcacttcga aagcaagctg gctgggttag ctttaaacag gccgttaccg acagcgatag
    12781 tccccagggc gatgaaaatc aggtcaggct taagtagcga caggccggtc atgaagtagc
    12841 caatcgccag cacaagtgct ccgagaacaa tggtgcgttt ggtccccagc aggtggtcgc
    12901 cgacatagcc gccaatggaa atgaggccat agaccagcgc agcaaaagca ccaaaagtga
    12961 caaaagcctg ttcttgcgag aatccaagct gcttaacgaa gaaaaccgcc agtacgccct
    13021 gcacgccgta gtagccgaat cgctcccata actcgacaaa aaagatcatg aaaaatgggc
    13081 gaggttgctg cagcatcccc atgggtgttg ttgtattcat atttattaac cttccaatac
    13141 catcctgaaa agtatgaacg cgtaagggat aacgcgcgaa aaagttttca tctgtcagag
    13201 attcttaata ttgatgcggt gaatagatta atacactatt acaacagaaa ataaccagct
    13261 gatcacacta atgatggaaa atatgatggt gttaaatgtt gtattggcag gggattttga
    13321 ttgcgtacgc agaccgtagg ccagataagg tgtttacgct gcaaggtaac aaggcgcgcc
    13381 aaaaagcaaa acgcccgcat atgcgggcgt tcaggaacac gtcgtagagg gaagattatt
    13441 cttcttcgtc gcgcagcgga acaatcagca tatcaacgtg aacggtgttg atcagctgac
    13501 gtgcggaaga catcagtttg ctccagaagt cctggtggtg accacaaacc accagatcca
    13561 tatcgtattt cttgattgca tcgaccagaa cctggcccag atcgccgctg ccgctcaggg
    13621 tttcagtgat tgggtagcct gcattagtgg aaagctcggt cagcgcatga tgtgtctctt
    13681 cagagatgcg tttctgcata tcacccagat tcacatcaat aagcccggtg tataggtcag
    13741 agtagtttac atctacgtgg atcagagaaa ctttcgcatt gtaggggcga gccatagaga
    13801 ctgctttctc taccagaact ttgctttccg gggagagatc gaccgcgatg agaatgtgtt
    13861 tataagccat agtgttactc cttccataaa gttgtcgatg actggccagc tagcgtttct
    13921 tgtgccgtta cgtcacccgc gtcctgcgaa ctacgtgcgc ggggctaacc cagccgtaaa
    13981 gattcagtgt taagaccatc cttaccttat agcgacccgg atgatccgtc aatccgcctt
    14041 gcttacccgt tagataaaca aattttcgac atgaatatat tgatagtggt taaccttctg
    14101 gaaaaaaaac aacctgatct cctacactat ctatagagcc gctcggatgt tggtcaccac
    14161 aaaagcggtt tctccaggac ctggtgttaa ctgattggca gcgtatcggg agcggtagcc
    14221 ccggggtgga actccgcggg caggtcgccg gggaggaggt atgataagca ccgtcgcatt
    14281 attttgggct ttatgtgtcg tttgcattgt taacatggcg cgctatttct catcactacg
    14341 cgcgttgtta gtggtactgc gtaactgcga tccattgctc tatcaatatg ttgatggagg
    14401 gggctttttt acctcacatg gccaacccaa caaacaggtg cgtctcgttt ggtatatcta
    14461 tgcccaacgt tatcgcgatc atcacgatga tgagtttatt cgccgctgtg agcgggtgcg
    14521 tcggcagttt attctgacca gcgcattgtg tggtctggtg gtggtcagtc tgattgcatt
    14581 gatgatttgg cattaagcac ttcagcaaaa ataacgcata aaaaaagcgg gccagtaaac
    14641 tgacccgctc gttttggtgc gtgcgattac aggaactgca aggagagcca gtaaagcccg
    14701 ccggaaagca gtaccgcagc cggaagggta aacacccagg ccatcagaat actggtcacg
    14761 gttttacgct gtaagccgcc gccatctacc accatcgtcc ccgcgacaga agaggagagt
    14821 acgtgggtag tggaaaccgg catcccggta taactcgcca ggccgataga cactgccgca
    14881 gtcatctggg cagacatccc ctgagcgtag gtcatgcctt tcttaccgat tttctcaccg
    14941 atagtcgttg ccacacgacg ccagccaatc atcgtaccga tacctaacgc cagcgcgacc
    15001 gccatgatga tccacaccgg cgcatactcg atggtgctaa gcatatcgga tttcagtttc
    15061 ttcaacaggc gctgatcgtc agcactcacg ccaggcatct tcaccacttt gtcgatagtg
    15121 tcagaaacgc acagcataat gcggcgcatc tggctacgtt gatcaagcga cagcttgtcg
    15181 tagctttcca catcggtagt caacatgcct ttcaggcggt tgagcgcgtt aatggtattc
    15241 gacggatggc aatggaactc cgcaggttgc gttgcgccag cttccggagc cggtactaac
    15301 tgatcagcac cggtagcctg tttcagcagc gcagggtgct gctcaaagta agcttccacg
    15361 ttgttgatgg catcacgggt acgggtgatt tcgtagccag tggcattcat gttcaccacg
    15421 aagcctgctg gtgcgacgcc aatcaatacc aacataacca gaccaatgcc tttctgacca
    15481 tcgttcgcgc cgtgcgaaaa cgccacgccg atagcggaaa ggatcagcgc aatacgcgtc
    15541 cagaacggcg gctttttctt gccgtctttc ttttcacgct ccgctggggt caggtggata
    15601 cgggcgcgtt tcttggtgcc gctccagtag cgacgcagca agaaaatcag accgccagca
    15661 aacaccaggc cgacaatagg ggaaacgatc agagaaccga aaatacttaa tactttcggg
    15721 atattgagtg catccaccac tgacgtcccg gtcatcaacg cattggttaa accaatcccg
    15781 atgatcgcgc caatcagcgt atgagagctg gatgcaggta aaccaaagta ccaggtaccc
    15841 aggttccaga taatcgccgc cagcaacata gagaacacca tggcaaggcc atgagacgat
    15901 cccatattaa gcagcagatc cgtcggcagc atatgcacaa tggcataggc aacactcaga
    15961 ccacccagca aaacacccaa aaagttgaac accgccgcca taaccacggc gagctgagaa
    16021 cgcatcgcgc gggtatagat aacggttgcc acggcgttgg ctgtgtcatg gaaaccattg
    16081 atggcttcgt agaacagcac aaaagccagt gcaagcaata ataacagccc ggtatgcaaa
    16141 tccaggccag caaacaaatg tagcatagga cgttacgcca ttttgaggac atgaacgcgg
    16201 cgcattatca gtgactttcg cggcgcgggc aaagtgaaat atagactttt tttgatttgc
    16261 ctcctgtatg gatttcactc aaaaaataat tatct
//
LOCUS       DAOTRK010000107        16122 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.107, whole genome shotgun sequence.
ACCESSION   DAOTRK010000107 DAOTRK010000000
VERSION     DAOTRK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.107"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(28..404)
                     /gene="rnpB"
                     /locus_tag="QIE41_003303"
     ncRNA           complement(28..404)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="QIE41_003303"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(437..1582)
                     /gene="garK"
                     /locus_tag="QIE41_003304"
     CDS             complement(437..1582)
                     /gene="garK"
                     /locus_tag="QIE41_003304"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDS3532156.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNVG"
     gene            complement(1679..2569)
                     /gene="garR"
                     /locus_tag="QIE41_003305"
     CDS             complement(1679..2569)
                     /gene="garR"
                     /locus_tag="QIE41_003305"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDS3532157.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIAEVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2599..3369)
                     /gene="garL"
                     /locus_tag="QIE41_003306"
     CDS             complement(2599..3369)
                     /gene="garL"
                     /locus_tag="QIE41_003306"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDS3532158.1"
                     /translation="MNNNVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3385..4719)
                     /gene="garP"
                     /locus_tag="QIE41_003307"
     CDS             complement(3385..4719)
                     /gene="garP"
                     /locus_tag="QIE41_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDS3532159.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5094..6665
                     /gene="garD"
                     /locus_tag="QIE41_003308"
     CDS             5094..6665
                     /gene="garD"
                     /locus_tag="QIE41_003308"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDS3532160.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6814..7149
                     /gene="prlF"
                     /locus_tag="QIE41_003309"
     CDS             6814..7149
                     /gene="prlF"
                     /locus_tag="QIE41_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDS3532161.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDTQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7149..7613
                     /gene="yhaV"
                     /locus_tag="QIE41_003310"
     CDS             7149..7613
                     /gene="yhaV"
                     /locus_tag="QIE41_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDS3532162.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH"
     gene            complement(7668..8477)
                     /gene="agaR"
                     /locus_tag="QIE41_003311"
     CDS             complement(7668..8477)
                     /gene="agaR"
                     /locus_tag="QIE41_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDS3532163.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8726..10006
                     /gene="kbaZ"
                     /locus_tag="QIE41_003312"
     CDS             8726..10006
                     /gene="kbaZ"
                     /locus_tag="QIE41_003312"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDS3532164.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10029..10502
                     /gene="agaV"
                     /locus_tag="QIE41_003313"
     CDS             10029..10502
                     /gene="agaV"
                     /locus_tag="QIE41_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDS3532165.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10513..11292
                     /locus_tag="QIE41_003314"
     CDS             10513..11292
                     /locus_tag="QIE41_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDS3532166.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11282..12160
                     /locus_tag="QIE41_003315"
     CDS             11282..12160
                     /locus_tag="QIE41_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDS3532167.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12178..12612
                     /locus_tag="QIE41_003316"
     CDS             12178..12612
                     /locus_tag="QIE41_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDS3532168.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12609..13742
                     /gene="nagA"
                     /locus_tag="QIE41_003317"
     CDS             12609..13742
                     /gene="nagA"
                     /locus_tag="QIE41_003317"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDS3532169.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRDGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTDVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14093..15247
                     /locus_tag="QIE41_003318"
     CDS             14093..15247
                     /locus_tag="QIE41_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDS3532170.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRVILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMVSCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15260..16120
                     /gene="kbaY"
                     /locus_tag="QIE41_003319"
     CDS             15260..16120
                     /gene="kbaY"
                     /locus_tag="QIE41_003319"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDS3532171.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         3848 a         4311 c         4157 g         3806 t
ORIGIN      
        1 ccgccgaagc gggtttttac gtaaatcagg tgaaactgac cgataagccg ggttctgtcg
       61 tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct acccgggttc
      121 agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag tttaccgtgc
      181 cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc ttacctgatc
      241 ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac tggtcgtggg tttccccccc
      301 aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc gcccgtctcc
      361 cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg cagtatagag
      421 ggtttgcgcg cccttgtcac cccacgttgc gcattccaat agccagtgtc gcggcgatat
      481 tacgtgaagc acggtaaata ttgtcataag ccccgcggaa tgcttcgtcc aacgtaccaa
      541 tgctggtcaa tacgctgaag accgcatcaa tgccatgctg atgtacaacg ccaacatcat
      601 tggtcaggct acccgcaatg ccaatcaccg gtttatggta ctttttcgcc acgtttgcga
      661 cgccaatcgg aaccttgccg tgaatactct ggctgtcaat acgcccttca ccggtgatca
      721 ccagcgtaca atcgtgaata tgttcctcca gattcagcgc cgtcgtgacg atttcaatac
      781 cacttttcag ttccgcacca agaaacgcca tcagcgccgc gcccatacct cccgcagctc
      841 ctgcgccggg gacatctttc acatcaacat gcagcgcttt tttaatgaca tcggcatagt
      901 gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt tgtgggccaa
      961 agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa gcgacgcgaa
     1021 tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc agagtattaa
     1081 gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc gcccccagcg
     1141 cctgtaccat gcctgcaccg ccatcatttg tagcgctacc gccaatgccg ataataatgt
     1201 ttgtcgcgcc gctctccagc gcctgcagaa ttaactcgcc tgtgccgcgt gaagtggtca
     1261 cgagtggatc acgtttttcc gcaggtacca gctccagccc actggccgcc gccatttcaa
     1321 taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc tcgcccagcg
     1381 gccctgtaac ccaggcgtga cgttcggaac cctgggtggc tgcaatcatc gcttccaccg
     1441 ttccttcgcc accgtcggca acaggaacag aaacgtactg tgcatcagga aaaatttccc
     1501 gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct ttataagagt
     1561 ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg agggcgtttc
     1621 tcttattcgg cctggattcc aggcccggat tgcaatacgc catccgggca cgacgtcatt
     1681 aacgagtaac ttcgactttc gccagttttt cgtagtagca cgccagggcg ctatgatctg
     1741 ccgttcctaa accatctgct cgcagtgcct gcatcatctc cataaccgca gctgtgagcg
     1801 gcagttgcgc gccgacgccg tgggaagtat ccagcgcatt tgccagatcc ttaatatgca
     1861 gatcaatacg gaagcccggc ttgaagttgc ggtccatcac catcggcgct ttggcatcca
     1921 gcacggtact gcccgccagc ccaccgcgaa ttgcctgata caccagatcc gggttaacgc
     1981 ccgctttggt tgccagcgtt aacgcttctg acatcgcggc aatattcagc gccacaatga
     2041 cctgatttgc cagtttggta acgttacctg caccaatttc cccggtatgc accacggaac
     2101 ccgccattgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg ccgcccacca
     2161 tcactgacag cgtaccgtcg atagctttcg gttcaccgcc gctcaccgga gcatccagca
     2221 tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc ggtgcgatag
     2281 aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca ttctcaccca
     2341 gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg tcgcactgtt
     2401 cagcgatcgc tttagccgta gaagctgttt ctgcacctgc agcaatcact tcagcaatag
     2461 cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg tttttactca
     2521 ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata tcaatcatct
     2581 ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttctgag tggcagagcg
     2641 gaagacgccg agatcgctgc cgacagccac aaacgtcgcg ccccattcca gataacgacg
     2701 cgcatcggct tcgaccggtg cgaggatacc gctgggtttg ccgtgcgcac tggcacggtt
     2761 aaaaatatgc tggattgctt tttgtacatc cgggtgcgag gcattgccga gatggcctaa
     2821 tgccgcagcc agatcgctgg ggccgacgaa gataccgtct acgccttcgg tagcggcaat
     2881 ggcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatgg tgatgttctt
     2941 gttcgactga gcgaaataat ccgccacggt gccaaacata ttggcgcggt gagaaacgga
     3001 gacgccgcga atgccttccg gtgggtaacg ggttgatgcc accgcctgct ctgcttcctc
     3061 ttttgtttct acaaaaggaa tcaggaagtt ataaaaaccg atatccagaa gacgcttaat
     3121 aattaccggc tcgttggtcg gcactcgcac tactggcgcg ctggcgctgc ctttcagggc
     3181 cattaactgc ggaataaacg tggagatatc gtttggcgca tgttcgccat ccagcaccag
     3241 ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga gtgctgacca
     3301 gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg ggaaaacatt
     3361 gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa tgtcgccaac
     3421 tacgaagagg tagcagacca tcgccatcag cgctgaacat cccacgaaaa ccagtgctgc
     3481 attgaaggag tgcagttcac ttaccagata gccaatcacc agtggagtga caatggaggc
     3541 aacattgcca aagacgttaa agacgccgcc gcagagacca acaatctctt tcggcgcggt
     3601 gtcagaaatc actggccagc ccagcgcacc aaatcctttg ccaaagaaag ccagcgccat
     3661 cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg tggaagccag
     3721 caacattccc agcacaatcg gtagcttacg tgccagggtc agggacaaac cgcgtttgat
     3781 cagataatcc gagaagacac ctcccagcac gccgcccgca aagccacaca gtgccggaat
     3841 cgaggcgacc agacccactt tcagaatcga catgcctttt tcctgcacca gataaattgg
     3901 gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga aaaatacgcc
     3961 cagcatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggac cgcttgctgc
     4021 cgcactgccc ggctttttgt ggtccatatc gaccaccgcg ccattttctg agataaactt
     4081 cagctcttcc gcagacatac gtgggtgatc tgtcgggtta tgaatcaact tgatccacag
     4141 cgccgtcagc acaaaaccaa tcacccccat aacggtgaag acgtgctccc agccccaggc
     4201 gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc gagaaatatt gcgccgagtt
     4261 aaagatggcg gaggcagtac cacgttcttt tgtcggaaac caggcggcga caatacgggc
     4321 gttcgccggg aatgatggcg cttccgagaa gccgagcata aagcgcataa agaacatgga
     4381 gatccctgcc caggccagcg ggaacatatc aacaaagcct tgcaggaaag tgaacagcga
     4441 ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa gcaaccagcc
     4501 gccggggatt tgcatcagca ggtaggccca gccaaaagcg gagaagatgt aacccatcga
     4561 aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca gcgttgcacg
     4621 atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat agcgggtatg
     4681 cacgcctttc ttttttacgt caacggtgtc cagaatcatt ttatttacct cgggtactta
     4741 tgctgatttt tattattatg gggaaggtgt tatttatgag ttttatttat gccgttacgg
     4801 caatgaactc gggaattagt ataagcagcg cgagaataat aatcattgtg caaatgctaa
     4861 tttaattaat actatttaaa tattattttg agcatatgca cataaggttg cgcgctaaag
     4921 cacagatttg cgctttacct taccgtgcgg cactgcaatc cctgaaatga ttgacattga
     4981 tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaagt catttatttt
     5041 taaatgagac caggtcctca ttttaataac ccctggctgg agaatattgc acaatggcca
     5101 acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac gacacagata
     5161 atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt ccggatgggc
     5221 tggaattaat tgaacatatt ccccaggggc ataaagtcgc attgctggac attccggcta
     5281 atggtgaaat tattcgttat ggcgaagtga ttggttacgc cgtgcgcgca atcccacgcg
     5341 gaagctggat cgacgaatca atggttgtac taccggaagc gccgccgtta cacacgctgc
     5401 cactggcaac caaagtcccg gcacccttac cgccactgga aggatacacc tttgagggct
     5461 atcgcaatgc cgatggcagc gtgggcacta aaaacctgct cggcatcacc accagcgtcc
     5521 actgtgtggc aggcgtggtg gactatgtag taaaaatcat tgaacgcgat ctgctaccga
     5581 aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggttgt ggcgtggcga
     5641 ttaacgcacc ggcggcagtt gtgcctattc gtaccattca caatatttcg ctgaacccta
     5701 actttggcgg cgaagtaatg gtgattggtc tgggttgtga aaagttgcag cctgaacgcc
     5761 tgctgactgg aacggatgat gtgcaagcta ttccagtaga aagcgccagc attgtcagtt
     5821 tgcaggatga aaagcatgtc ggttttcagt ccatggtcga ggatattttg caggtcgccg
     5881 aacgccattt gcacaaactg aatcaacggc agcgagaaac ctgtccggct tcagaactgg
     5941 ttgtcggcat gcagtgcggc ggtagcgatg cgttttctgg cgtaacggca aacccggcgg
     6001 ttgggtatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttt tcagaagtca
     6061 cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa gaggtcggta
     6121 aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga aaaaccgacc
     6181 gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct ggcaaacgtg gtggagaaag
     6241 cgctcggctc cattgctaaa tcgggtaaaa gtgcaattgt tgaagtgctg tcgcctggtc
     6301 aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg
     6361 gcacgcaaca ggtggcttcg ggtatcaccg tgcaagtgtt tacgaccggt cgcggtacgc
     6421 cgtacggcct gatggcggta cccgtcatta aaatggcgac ccgcaccgag ctggcgaacc
     6481 gctggtttga tttaatggat attaatgcgg gcaccatcgc taccggcgaa gaaactattg
     6541 aagaggtggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg aagaagaaaa
     6601 ccttctcaga tcaatgggga ctgcataacc agctggcggt gtttaacccg gcaccggtga
     6661 cctgatttct tttggaacgt ccctcgcaaa acatggcctt agtgccatgt ttttattgtt
     6721 taaagccccc acgtacatta ataatgcatt tgcattacct tagttcaagc ttataatttg
     6781 agcagaaaac aggctgtaaa aggacagtga atcatgcccg ctaatgctcg ctctcacgct
     6841 gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa caactatccc cgcgccagtg
     6901 cgtgaggcct taaaactgaa gccaggccag gacagcattc attacgaaat tctgcctggt
     6961 gggcaagtat ttatgtgccg actgggagat gaacaggagg atcatactat gaatgcattt
     7021 ttgcgttttc tggatgcaga tatccagaac aacccgcaaa aaactcgtcc attcgacact
     7081 caacaaggaa agaaacttgt cgctggcatg gacgtcaaca ttgatgatga gattggcgac
     7141 gacgaataat ggattttcca caaagggtta atggttgggc gctatatgct catccctgtt
     7201 ttcaggaaac ctacgacgct ttagttgccg aagtcgagac attaaaggga aaagatcctg
     7261 aaaattatca gagaaaagcc gccacaaagt tattggcggt agtccataaa gtgattgagg
     7321 agcatatcac ggtcaatcca tcatcaccgg cattccgtca tggcaagtcg ttaggctctg
     7381 ggaaaaataa agactggtca cgggtaaaat ttggtgctgg tcgttatcgt ctcttctttc
     7441 gttacagcga aaaagagaaa gtcatcattc tgggatggat gaacgatgaa aacacgctgc
     7501 gcacctacgg taaaaaaaca gatgcctata ccgtattcag caaaatgtta aaaagaggac
     7561 atcctcctgc cgactgggaa accctcaccc gagagacaga agaagcccat tgatggtgtt
     7621 cacaatgccc gccgaacgca ttcgtgttga ccagcgggca ccctttctca ctccccgacc
     7681 agaatcactt caaccccagc ctttcgcagg ccttccagac tatccgcagg aatgccttca
     7741 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacggttg
     7801 aacttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggttt
     7861 aaacgggctt catcttcatt atgcgtgctg acgccgcgct ccagatcgat cgcatctacg
     7921 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gttcagcctg atcgccgtaa
     7981 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     8041 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtgcttg
     8101 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     8161 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcattgcggt gttcagcgcg
     8221 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgccg
     8281 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcgtt gcggatcgtc
     8341 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacacttcc ttgctggcgc
     8401 aggcgttgaa tgatctgctc tcgtcgctca ctggtgcctg tcactcgctt ctcgcctgaa
     8461 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     8521 attaacgcct ttctattaag caaatgcaag cccaccttac ccattggcgc aagctactct
     8581 cgtttcagtg gctttcatta tgtttctttt gtgaatcaga tcagaaaacc attatctttc
     8641 gttttatttt tatctcacca tggcgcagta tcaactgaaa caaaacgaaa gattaatatc
     8701 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     8761 agcgggcaaa acaaatggaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     8821 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     8881 ggtagaccag ttcggcggct ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     8941 tcagctcgcc gactcgttga atttcccgca ggatgcgttg attctgggtg gtgaccatct
     9001 gggtccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     9061 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     9121 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     9181 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     9241 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     9301 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaggg
     9361 tctgagtgcc atctggccac gcatcattgc tctggtggtt caacccggcg tcgaattcga
     9421 tcacaccaac gttattgatt atcagcccgt caaagcgacc gccttaagcc agatggtcga
     9481 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cacaatcgtt
     9541 gcgccagctg gtgattgacc actttgccat cctgaaagtt ggcccggcgc tgaccttcgc
     9601 cctgcgtgaa gctctgttct ctctggcggc gattgaagaa gaactggtac cagcaaaagc
     9661 ctgttctggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     9721 aagccactac cacggtgacg gcaacgcacg tcgcctggcg cgtggttaca gctactcaga
     9781 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     9841 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatttgc cgctgcaata
     9901 cgtgaaagtt cgctccggcg agctgcagcc aacaccacgg gaactcatta tcaaccatat
     9961 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
    10021 aacacgctat gccaaatatt gttttaagcc ggattgatga gcgtttgatt cacggtcagg
    10081 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
    10141 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
    10201 tacgtttctg gacgctgcaa aaagttatcg acaacatcca ccgcgccgcc gatcgacaga
    10261 aaatcctgct ggtttgtaaa acacctgccg atttcctgac gctggtgaaa ggtggcgttc
    10321 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaagcaa caaatcgcca
    10381 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
    10441 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
    10501 gagggattca tcatggaaat cagtctgttg caggcattcg cgttgggcat tatcgccttt
    10561 atcgctggcc tggatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
    10621 ccgttagtcg ggctggtact tggcgatctg cataccggta ttttaaccgg cggtacgctg
    10681 gaactggtgt ggatggggct ggccccgctg gcaggcgcac aaccgcctaa cgtgattatc
    10741 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
    10801 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
    10861 atgtctggcg taatgtcccg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
    10921 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10981 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    11041 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    11101 ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca tcctgggctt tgttgccgca
    11161 gcctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    11221 gacctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    11281 gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa aacgaagaaa cactgctgac
    11341 tggcgtcaat gaaaacgtgt atgaagatca gagcattggc gcagagctga caaaaaaaga
    11401 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    11461 gcaggcttcc ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    11521 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    11581 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    11641 tattcagagc accaaaattg ccgtcggtgc gccgctcggc gggattggcg atgcaatgtt
    11701 ctggctgacg ctactgccga tttgtggcgg gattggtgca agcctcgcgc tacaaggctc
    11761 tattcttggc gctgtcgtct ttattgtgct gttcaacgtg gtacacctgg ggctgcgttt
    11821 tggtctggcg cattatgctt atcgcatggg cgtggcggcg attccactca ttaaagcgaa
    11881 taccaaaaaa gtcggccatg ctgcgtcaat cgttgggatg acggtaatcg gcgcgctggt
    11941 agcaacctat gttcgtttaa gcaccacgct ggaaatcacc gctggcgatg cagtggttaa
    12001 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    12061 gaccatgttc tggctggtac gccgcggctg gagtccgctg cgcctgattg cagttaccgt
    12121 ggttctcggt atcgtcggta aattctgtca tttcctttaa atacaatgag gtattagatg
    12181 ttaagtatta ttttgacagg gcatggcgga tttgccagcg gcatggaaaa agcaatgaag
    12241 caaatcctcg gcgagcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcacc
    12301 gcgcagctta catcgcagct tgaagaggcc atcgcccaac tcgattgtga agacggcatt
    12361 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    12421 caaaaaccgg gctgtgaagt gatcaccggc accaatttgc aactgctact ggagatggtg
    12481 ctggagcgcg aagggttaag cggtgaagag ttccgtgtgc aggcgctgga gtgtggacac
    12541 cgtgggctga ccagtttggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    12601 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggctcg
    12661 atgaccatca gttgcgtatt gctgacggtg taatcgcagc aatcgaaccg attccagtgg
    12721 gcgtgactga acgcgatgcg gaactgctct gccccgctta catcgacacc catgtacacg
    12781 gtggtgcggg cgttgatgta atggatgacg cgccggatgt gctcgacaag ctggcaatgc
    12841 acaaagcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    12901 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcgacgga cctggtgcgc
    12961 aagtgctggg gagttatctc gaaggaccat acttcacgcc gcagaataaa ggcgcgcatc
    13021 cgccggagct gtttcgcgag ctggaaattg ccgagctgga tcaattgatt gccgtttctc
    13081 agcacacctt gcgcgtggta gcgctggcac cggaaaaaga aggggcattg caggccatcc
    13141 gccatcttaa acagcaaaac gtacgggtga tgctggggca tagcgcggcg acctggcaac
    13201 aaacccgtgc cgcgtttgac gctggtgccg acggcctggt gcattgctat aacgggatga
    13261 caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg attaacggac aagcgcgcct
    13321 ggctggaact gatagccgat ggtcatcatg tgcatccggc ggcgatgtcg ctgtgttgtt
    13381 gctgtgcgaa agagagaatc gtgctcatca ccgacgcgat gcaggcagcc gggatgccgg
    13441 atggtcgcta tacgttatgt ggcgaagaag tgcagatgca cggtggcgtt gtccgtaccg
    13501 cgtccggtgg actggcgggc agtacgctgt ctgttgatgc ggcagtgcgc aatatggtcg
    13561 agttgacgga cgtaacgcct gcggaagcca tccatatggc gtcgctgcat ccggcgcgta
    13621 tgctgggtgt tgatgatgtt ctgggatcgc ttaaaccggg caaacgcgcc agcgtcgttg
    13681 cgctggatag cgggctgcat gtgcaacaaa tctggattca gggtcaatta gcttcgtttt
    13741 gatggtttgc tcctttattg ggccttcact tcccccgtaa ggcctttctt tttctttctt
    13801 tttgatctgt acagcggtgt cggatgcgac gctgacgcgt cttatgcgac ctacggtacg
    13861 ccgcatccgg caatcggtgc acatagcaac gtcatcatgc aattctttaa tcttcccttg
    13921 tcttttcttt caacgatcac aaatttcgtt ttatttcttt tttctccatt gaactttcag
    13981 tttcttttct atagatttta atcaacgaaa gacatcacca agtgaaatga aacgaaaggt
    14041 aagcgaaagc gacaacgccc gacgtcaagt tcatcagact aaggattgag ttatgccaga
    14101 aaattacacc cctgctgctg ccgcaaccgg tacatggact gaagaagaga tccgccatca
    14161 gcctcgcgca tggatccgtt cactcaccaa catcgacgcg ctacgttccg cgctcaataa
    14221 cttccttgaa ccgttactgc gcaaagagaa tctgcgggta atcctgaccg gagccggaac
    14281 ctcggcattt atcggtgaca tcatcgcgcc gtggctcgcc agccatacag gtaaaaactt
    14341 cagcgccgta ccgaccaccg atctggtcac caatccgatg gactacctga atccagctca
    14401 tccactgctg ttgatctcct tcggtcgatc cggcaacagc ccggaaagcg tcgccgccgt
    14461 ggaactggca aatcaatttg tacccgagtg ctatcacctg ccgatcacct gcaacgaagc
    14521 gggcgctctt taccaaaacg cgatcaacag cgacaacgcg tttgccctgc tgatgcccgc
    14581 agaaacgcac gaccgcggct ttgcgatgac cagcagcatt accaccatga tggtcagctg
    14641 cctcgcggtt ttcgcacctg agacgatcaa cagccagagc ttccgcgacg tggcggatcg
    14701 ttgccaggcg atcctgacct cactgggcga tttcagcgaa ggtgtgtttg gttacgcacc
    14761 gtggaaacgg atcgtttatc tcggcagcgg tggcttacag ggcgcagcac gcgagtcggc
    14821 gctgaaagta ctggaactga cggcgggtaa actggcggcc ttttatgatt ccccgaccgg
    14881 attccgtcat ggcccgaaat cgctggtcga taacgaaacg ctggtggtgg tatttgtctc
    14941 cagccaccct tacacccgtc agtatgatct tgatctgctg gctgaacttc gccgcgataa
    15001 ccaggcgatg cgcgtaatcg ctatcgccgc ggaaagcaac gacgttatta ccgccggtcc
    15061 acatatcatc ctgccgccgt cccgtcactt tatcgacgtt gagcaggcat tttgcttcct
    15121 gatgtacgcc cagacgtttg cactgatgca gtcgctgcac atgggcaata cgccggatac
    15181 cccatcagcc agcggcaccg ttaaccgcgt ggtgcaaggc gtaatcattc atccgtggca
    15241 ggcataagag gatcgcatta tgagcattat ctccaccaaa tatctgttac aggacgccca
    15301 ggccaatggc tacgcggtgc ctgcttttaa cattcataac gccgagacga tccaggcgat
    15361 cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc gccgggacgc cggggacctt
    15421 taaacatatc gcgctggaag agatctacgc cctgtgtagt gcctattcca caacctacaa
    15481 catgccactg gcgctgcatc tcgaccacca cgaatcgctg gatgatattc gccgtaaagt
    15541 ccacgcaggc gtgcgcagtg cgatgatcga cggcagccac ttcccgtttg ccgagaacgt
    15601 aaagctggtg aaatcggttg tcgacttctg ccactcacag gattgcagcg tggaagcaga
    15661 actgggccgc ctgggcggtg ttgaagatga catgagcgtt gacgccgaaa gtgcattcct
    15721 gaccgatcca caagaagcta aacgctttgt cgaactgact ggcgtcgaca gcctggcggt
    15781 agcgattggt acggcgcacg gcttatacag caaaacgccg aagattgatt tccagcggct
    15841 ggcggaaatt cgtgaagtgg tggatgttcc tctggtgctg catggtgcca gcgatgtccc
    15901 ggatgaattt gtccgtcgca ctattgaact tggcgtcaca aaagtgaacg ttgccacaga
    15961 attaaaaata gccttcgctg gcgcggttaa agcctggttt gcggaaaatc cgcagggtaa
    16021 tgatcctcgt tattatatgc gcgtcggtat ggatgcgatg aaagaagttg tcagaaataa
    16081 aattaatgtc tgtggttcag cgaatcgaat ttcagcataa tc
//
LOCUS       DAOTRK010000108        15981 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.108, whole genome shotgun sequence.
ACCESSION   DAOTRK010000108 DAOTRK010000000
VERSION     DAOTRK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.108"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            96..662
                     /locus_tag="QIE41_003320"
     CDS             96..662
                     /locus_tag="QIE41_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDS3532172.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            666..1241
                     /locus_tag="QIE41_003321"
     CDS             666..1241
                     /locus_tag="QIE41_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDS3532173.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PLATFTSTSVKKDGDDLDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1283..2335)
                     /locus_tag="QIE41_003322"
     CDS             complement(1283..2335)
                     /locus_tag="QIE41_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDS3532174.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2560..3480
                     /gene="lpxL"
                     /locus_tag="QIE41_003323"
     CDS             2560..3480
                     /gene="lpxL"
                     /locus_tag="QIE41_003323"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDS3532175.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3652..4878
                     /gene="mdtG"
                     /locus_tag="QIE41_003324"
     CDS             3652..4878
                     /gene="mdtG"
                     /locus_tag="QIE41_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDS3532176.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4961..5335
                     /locus_tag="QIE41_003325"
     CDS             4961..5335
                     /locus_tag="QIE41_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HDS3532177.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWGEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5336..5563)
                     /locus_tag="QIE41_003326"
     CDS             complement(5336..5563)
                     /locus_tag="QIE41_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDS3532178.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5736..8279)
                     /gene="mdoH"
                     /locus_tag="QIE41_003327"
     CDS             complement(5736..8279)
                     /gene="mdoH"
                     /locus_tag="QIE41_003327"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDS3532179.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDADHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKSWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8272..9807)
                     /gene="mdoG"
                     /locus_tag="QIE41_003328"
     CDS             complement(8272..9807)
                     /gene="mdoG"
                     /locus_tag="QIE41_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDS3532180.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYDAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10201..11358
                     /gene="mdoC"
                     /locus_tag="QIE41_003329"
     CDS             10201..11358
                     /gene="mdoC"
                     /locus_tag="QIE41_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDS3532181.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTFAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11366..12847)
                     /gene="clsC"
                     /locus_tag="QIE41_003330"
     CDS             complement(11366..12847)
                     /gene="clsC"
                     /locus_tag="QIE41_003330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDS3532182.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12789..13322)
                     /gene="ymdB"
                     /locus_tag="QIE41_003331"
     CDS             complement(12789..13322)
                     /gene="ymdB"
                     /locus_tag="QIE41_003331"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDS3532183.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENTHLYERLLTQQGDE"
     gene            complement(13417..13728)
                     /locus_tag="QIE41_003332"
     CDS             complement(13417..13728)
                     /locus_tag="QIE41_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDS3532184.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSSAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13849..14181)
                     /gene="csgC"
                     /locus_tag="QIE41_003333"
     CDS             complement(13849..14181)
                     /gene="csgC"
                     /locus_tag="QIE41_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDS3532185.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14239..14694)
                     /gene="csgA"
                     /locus_tag="QIE41_003334"
     CDS             complement(14239..14694)
                     /gene="csgA"
                     /locus_tag="QIE41_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDS3532186.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14735..15190)
                     /gene="csgB"
                     /locus_tag="QIE41_003335"
     CDS             complement(14735..15190)
                     /gene="csgB"
                     /locus_tag="QIE41_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDS3532187.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4012 a         4039 c         3760 g         4170 t
ORIGIN      
        1 aaaaataaat atcattcaat ttgttatttt tgtgttgcaa ttcattctcc gacacgccat
       61 tttctttctc tcgcaaattc gttttggatg cacttatgtc attcacaaat acccctgaac
      121 gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt
      181 ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac
      241 ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt
      301 ggcgcgtcat atctccaccg ccgggtccgc tgcagagtta ttcgcccatg actcgtcttg
      361 gcgcaaaggc tggacatctc gcattgtact tattgctgtt cgccatcggc atcagcggct
      421 atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg
      481 cgaccctcgc cgacgctggc gcacaggctg acttcgccgg tgccctgcat ttttggctcg
      541 cgtggagcgt cgtagtactg tccgttatgc atggatttat ggccctgaag catcatttca
      601 tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat
      661 aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcttc cctgatgttc tctgccggtt
      721 cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaacttcc
      781 gcatccagca tcttggctat agctggttat atggcacctt taaagatttc gacggtactt
      841 ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca
      901 gcgtcgatac taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata
      961 ctgcaaaata tccactggca acattcacct ccaccagcgt gaagaaagac ggtgacgacc
     1021 tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga
     1081 aattaattgg tcaaggcgac gacccgtggg gtggtaaacg tgcaggcttc gaggccgaag
     1141 gcaaaattaa gctcaaggac ttcaatatca aaacagattt aggtccagct tctcaggaag
     1201 tggatctgat tatttcagtg gaaggcgtac agcagaagta agcgtgaaat gttgccggat
     1261 ggcacgcatc cggcaatgat atttattctg ttggatcagg aatgcccagt gttgtattca
     1321 gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc
     1381 gctgttcttc aggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt
     1441 atttttccgc acatactgga cactgaataa aaagcaggtg gcagccatca tttttacagt
     1501 tggtatggct gtcgcacggc gcaccgcact ggtggcaatg cgcgataatc tcatccgata
     1561 tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgt accggtaaac
     1621 cctgctcgcg cgccttgcgg gcgtattcaa taattccgcc ctcgatatgc cagactttat
     1681 tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt
     1741 acataacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct
     1801 cacggaaggt atctgccgga atttccagcg cgttttcaaa gtgccccact tcatactcat
     1861 agtggttacg catgtcgata aacaatgcat cgggatcgtc aagcatggcg ttcacttccg
     1921 ccgcttgcag atactcaccc acattgctgg catcaaagtg aggatcgtca ataccgtcgg
     1981 caacgatacg atcgcggacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca
     2041 acgcgatatt caggcgtaag ccctctaaag ccgggtcgaa ggcataaagc tgcgcgcgaa
     2101 atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat
     2161 acactcgccc aaaaacatcc agcgcggtaa acagctgata taaagcgtca cgggtcgcct
     2221 taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcag
     2281 ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt
     2341 acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat
     2401 taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg
     2461 cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat
     2521 acatacaatt gcccgtatag gttgaaaaac aggattgata tgacgaatct acccaagttc
     2581 tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg
     2641 ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactt
     2701 gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc
     2761 ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc
     2821 atgggactga tggaaactgg catggcgtgg ttctggccgg accgcagaat cgcccgctgg
     2881 acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg
     2941 ttagttggca tccattttct gacactggag ctgggtgcgc ggcagtttgg tatgcaggaa
     3001 ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg
     3061 ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa
     3121 gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca
     3181 agcgttttcg tcccgttgtt tgccgttgag caggctgcga ccacgaccgg aacctggatg
     3241 ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc
     3301 aaagggtatc agttgattat gctgccgcca gagtgttctc cgccgctgga tgatgccgaa
     3361 accaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag
     3421 tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa
     3481 atctcccatg ccggatgctt cagaagggta tccggcatta ccacagcaaa tccccctgat
     3541 ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct
     3601 tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc
     3661 tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt
     3721 tttcttacgg gtgccgcctt cagcctggta atgcccttct tacccctcta cgttgagcag
     3781 cttggcgtta cgggccactc cgccttgaat atgtggtccg gtattgtctt cagcattaca
     3841 tttttatttt ctgccatcgc ctcaccgttt tggggtggac tcgccgaccg taaaggccgc
     3901 aaactcatgc tattacgctc tgccctcggc atgggcatcg tgatggtgtt gatggggctg
     3961 gcacaaaata tctggcagtt tttgatccta cgggcgcttc ttgggttact tggcggattt
     4021 gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag cggctgggcg
     4081 ctgggtacgc tctccacagg cggcgttagt ggtgcgttgc tcggcccaat ggctggcggc
     4141 ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag tgtgctcata
     4201 ctctgctttt tcgtcaccct gttttgcatc agagaaaaat tccagccggt cagcaaaaaa
     4261 gagatgctgc acatgcgtga agtggtgaca tcacttaaaa acccgaaact ggtactcagc
     4321 ctgtttgtca ctacgttaat catccaggtg gcaacgggct caattgcccc cattctgacg
     4381 ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag tggcatgatc
     4441 gcctcggtgc caggcgtggc ggctctgcta agtgcaccac gactcggcaa acttggcgat
     4501 cgaatcggac ccgaaaagat cctgattacg gcgctgatct tttctgtact gctgttgatc
     4561 ccaatgtctt acgttcagac gccattacaa cttgggattt tacgtttttt gctcggtgcc
     4621 gccgatggtg cactactccc cgccgtacag acactgttgg tttacaactc gagcaaccaa
     4681 atcgccgggc gtatcttcag ctataaccag tcgtttcgtg atattggaaa cgttaccgga
     4741 ccattgatgg gagccgcgat ttcagcgaac tacggtttca gagcggtatt tctcgtcacc
     4801 gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg tcgtcgaata
     4861 ccccaggtat cgaactgatt tttcgccttt catacttgta aaagcggaga atcagctatc
     4921 cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt acgcaacgct
     4981 tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg gcatcgacgc
     5041 cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg acggcgacat
     5101 catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt tacctatgct
     5161 tagcggtgaa gccgcgcaaa gtgtctttga tggcgactat gatgagatag agatccgcca
     5221 ggagtggcag gaagagaata cattacatga atggggcgag ggggaatttc agcttgagcc
     5281 accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac gttaattact
     5341 catacgggcc atgatgaatg tcgatcggca gcagtaaagt atcaaaaacc agggagaatg
     5401 gcagatcaag gattgtgaca taacgccagg cggagtcacg cacatcccat tgcacaccgg
     5461 gataatattg gttgccatgc ccctgccccg gtatagtgcg gctaatgatg ctgccacagc
     5521 cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac ctcttccgta
     5581 aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc agatgtggcg
     5641 tgaacgcctt atccgaccta caaaatcttg caaaatcaac acattgcagg agcgatgtag
     5701 gcctgataag cgtagcgcat caggcaacta cgtttttatt gcgaagccgc atccggttta
     5761 cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga agaatatctc
     5821 tcaggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc gcttagcagc
     5881 accaggcgac gatcgcgatt cagcttctct ggcgtctcgt tcagcgcctg ttcaacgtgg
     5941 cggtcacggg cgatttccag caccttactg gcgcggtgac gcgcggtcgc cattgcggtt
     6001 gccagagcgt taaatgacgg gttaaacact gcgtgcataa agccatcgtc aagggagcgt
     6061 tggcgattca tctcaaggaa ccgatcggta tcaaccagca cctgcggcgg tgaatactct
     6121 tccgggatca ggaacagttt ccagcttttg gtgcgcagac caacggtggc acggctggaa
     6181 atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggtgccag ccagaacagg
     6241 aaacgcagat ccaaccacgc catcccaaca gcccacacta accctaacag cagctgtgag
     6301 ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg cggtgaattc
     6361 cacaccactt cccagccaag gaacgcgctg acgacgaaga ccgtatggaa cagcatgcgt
     6421 accggagcca gcagcacgga aaacagcact tccagcagca acgataatgt aacgcgccag
     6481 aagccgccat attctttcgt tcctttgcac caaataagca aaatgctcaa tagctttggc
     6541 aggaacaaca gcaccatggt cgaagcaaaa agtgcaatcg ccagctcagg acgccactgc
     6601 ggccacaccg ggaacaactg ccgtggttgc aggaagtatt gcggttcggt caacgcatgc
     6661 actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc ggagagataa
     6721 gacatcacgc ccgtcagaaa taccgcacgg tgaaccgggt gcataccctt caccaggaac
     6781 agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag ctcatcaagc
     6841 aagttaggcg gtaattcttc ataagaaccc gggagatcgt aagcaatcca gactccccaa
     6901 cctgcacggc gcatcaacgc agcttccacg aagtcatgtg acaggattga accggcaaag
     6961 gaaccttcgc ccggcagcgg agccagtgcg cagtgctcga taaacggttt cacgcggata
     7021 atcgcgttat gtccccagta gtgcgactcg ccaagttgcc agaagtgcaa accggctgta
     7081 aacagtggcc catacacgcg agtcgcgaac tgctgacagc gtgcatacag cgtatccata
     7141 ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc catcaaacgc
     7201 accaggccgc ataaacaatc accggtcatt accgagtcag catccagcac caccatgtag
     7261 ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt acgcttcacg
     7321 cggcgacggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc gataagctcc
     7381 atccaggctt tttgctctgc gacgcagata tccgggttat aactgtcact cagaatgtag
     7441 acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc acgcaagcca
     7501 gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt gcgatgctcc
     7561 gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc gcgaccaata
     7621 agcagttgca ggaaccccat caacgccgtc cagaatccgg cggacaccca acagaacagt
     7681 accgcaaaga ggatcaggat accggtttgc agcatataag gcagaagctg cataaaggaa
     7741 acccacaaat cctgaccaac catatccata ggattaatca gcgcccaacc ctgataagga
     7801 agaatggtct tcatatacca ggtcgcgacg acagtttgcg cgagcgtcag gatcaacaga
     7861 atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctctcctg ctcttctttg
     7921 gtcaaacgag ccagatagcg cggcgttaca tcgcgtccac gcaggcgatc ccagaaacgg
     7981 cctaccgggt tggtacgcca cgggtcggga aacatcgagg agcgttttgc ttctggcatc
     8041 gccttaagct gatcgcgccc ttcgtcgtct ttaattaact gtccatcagc aagtgaatct
     8101 ggccaggctt gttccagacg cgcctttacc gagccttgcg gggagtcatc ctcccgcgcc
     8161 caggtacggt ggtcggcatc cagcgcctga tgaacggcgc ggatatcagt cttcggcaat
     8221 gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt cttattcatt
     8281 ggcaggtaac tggtagctcc aggtttcact caacgtctga tcggcattca ccagcgcagc
     8341 acgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca gacgccagcc
     8401 tttggtaacc gggttatagc gcaccgtgct ttcaactatc tcaccattat caccaatgct
     8461 ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc cggtaaaatc
     8521 gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg ttcgactgct tcacatcccc
     8581 cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt tgtcttcatc
     8641 acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg gcagctgatc
     8701 cggcgtccag taagcgacga tgttatcgtt ggtttcatcg ttggttggaa tttccaccag
     8761 ctcaacgctg cctttgcccc actccccttt cggagtcacc catgcgcttg gacgaagatc
     8821 gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca acagaccgaa
     8881 gccttgcggg ttttccatgg agaagctgct gaccgctaaa tgtttcgggt tattcaacgg
     8941 acgccagatc cattcgccat taccagcatg gatagagaga ccgttagagt cgtgcaactc
     9001 cggacgatag ttatttgcag gcgacggttg gttcggccca aacaggaaca tactggttaa
     9061 cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact gcacatccac
     9121 aaccgtgtca cgccctggca taactacgaa tttgtaagca cctgtcgcgc gcggcgagtc
     9181 aagcaatgca taaatggtta aacgtttatc agtcggtttt ggacgctcga tccagaactc
     9241 tttgaagcgt ggaaattctt cacccgatgg caaggcggta tcaattgcca ggccgcgtgc
     9301 agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc cgagcatgct
     9361 gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa aaccggcaaa
     9421 accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg ccgaaagtga aataatccgg
     9481 gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga ccggggtatc
     9541 gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat tgttccagta
     9601 cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatgt cgcggaaaac
     9661 ggagggcaag ttgcttttgg gcgcatcata gcctttcccg gctaaggatt gagcttgctt
     9721 tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta acattactgc
     9781 agcactcaac caacgcattt tcatcatttg tagtttatgt ttcataatta gtaagcactt
     9841 ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc cattccgaca
     9901 acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga ttgcaaccgt
     9961 tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg gtttgccagc
    10021 aaatttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta gcgatgatgc
    10081 tatgcataaa aaccttgaag cctgcgcctg gcgcggtcag gtcggtgtcg ctatggcaac
    10141 aagcctaaag gcataacccg acagggataa gacgaaaaat ccgagattag ttaaccatat
    10201 atgaatccag tacccgcgca acgtgaatat ttcctcgact ccatccgcgc ctggctgatg
    10261 ttgttaggga tcccttttca tatttcttta atctattcga gccatacatg gcatgtgaat
    10321 agcgccgaac cgtcattatg gctgaccctt tttaatgact tcatccactc gttccgcatg
    10381 caggtatttt tcgttatatc cgggtacttt tcctacatgc tttttttacg ctatcccttg
    10441 aaaaaatggt ggaaagtacg tgtcgaacgt gtaggtatcc cgatgttaac agccatcccc
    10501 ctactgacat tgccgcaatt tattatgctg caatacgtca aagggaaagc ggaaagttgg
    10561 cctggactgt cattgtatga caaatataat acgttggcct gggaattaat atcacacctg
    10621 tggtttttac tggtgttagt ggtcatgacg acgctgtgcg tctggatatt caaacgtatc
    10681 agaaataatt tagaaaattc tgataaaacg aataaaaaat tctcgatggt aaaactatcg
    10741 gtgatttttt tgtgcctcgg catcggttat gcggtaataa gaagaacaat ttttattgtg
    10801 tatccgccca ttctgagtaa cggcatgttc aattttattg tcatgcaaac gctattttat
    10861 ttgccgttct ttatcctcgg cgcactggct ttcattttcc ctcatcttaa agccttgttt
    10921 accacgccgt ctcgtggctg taccttcgca gcagcattgg catttgtcgc ttatttactc
    10981 aaccagcgct atggcagtgg cgatgcctgg atgtacgaaa ccgagtcggt gatcaccatg
    11041 gtcctcggtc tgtggatggt gaatgtggtc ttctccttcg gccaccgttt gcttaacttc
    11101 cagtcagcgc gggtgactta ctttgttaat gcatcgctgt ttatctatct ggttcaccac
    11161 ccgttaacgc tgtttttcgg cgcgtacatt acaccgcaca tcacatccaa ctggcttggt
    11221 tttctctgtg gcctgatatt cgtagtaggg attgcgataa ttctgtatga aattcattta
    11281 cgcatcccgt tactgaagtt tttgttctct ggtaaaccgg ttgttaagcg tgagaacgat
    11341 aaagcaccag cccgttaagc cgcgtttata ataaccattc cacaggcaat atcgacgcca
    11401 gtctgaccat aacccgcttc cagaaactgg tggcgggttc ttttttgaga acaatttctt
    11461 tctttgcatg acgatcaacc cagttgatcc gtccccacct gtccagacgg agctgccagg
    11521 ccgcatcata ctggctctga ataaagcgtt tatcaattaa ctgtgccagc gtttcgctct
    11581 ctatcacgaa ccccatttca gtattgagca atgtcgaacg cggatcgaaa ttgaaagagc
    11641 cgataaacac cgttttacca tcgatgctaa aggttttagc atgcaggctg gcaccggaat
    11701 taccggttat gccgcgatcg tgtaacgtac tactttgttc acgcgtcggc ttgagttcat
    11761 ataattccac gccgtagcgg agcaattttt tgcgccagcg cgcgtatccg gcatggacga
    11821 cagcaacatc gttagcggca agagaattgg ttaggatcgc aatctttacc ccttttctca
    11881 ccatccgtaa gagttgcgcc acacctgcgc gtgtcggtac aaaataggaa gagataatat
    11941 cgatgcgttc actgggtgag cccatgatat cgaacaggcg ctgcggtaac agtgaatgcc
    12001 gttttgcctt gccctcccct ttcgccggat catcacttaa taaacgtgtt ttcgcccaga
    12061 taagcggcaa cgttccatca accagatgat ttataaacgg gctggattcc attttgcgta
    12121 aataacgatg cgtcatggca tcgttgtgcc aggatgctgg taactcgatg cgatccgcca
    12181 tttcaccttc cgggacatcc agcacctgct gtaggggtga aaccgatttg caataccagt
    12241 agcgggcgaa atcatcggca acgtcctcta caactggtcc tattgccatg acatctaaat
    12301 ccgaaaaaag tggctcttcc cctgcaccaa aataggcatc accaatattt cgccccccta
    12361 ccagcgtcac cacgccatca acagtgaaac ttttattgtg catacggcga ttgagacggg
    12421 aaaagtcggt gatataacca agcggacgta gcaagcgaaa cgagaaagga ttaaaaagcc
    12481 ggacttcaat gcggggatga ctgtcaagca agcgtaaaat gtcgtcaagt ccgggtgtat
    12541 tgttgtcgtc cagcaacaaa cggacgcgaa cgccacgctt tgctgcggct aacagggcgg
    12601 aaaagagtaa ccgacccgac atatcgtcct gccagatgta atactgaaca tccagcgtat
    12661 gctctgccat ttcggccaga cgataccggg cggcaaacgc atccagactt ttctccagag
    12721 ggaaaaggcc acactgaccg ggatgttgcg aacacagtgg cagcaccgcg ctcgccagcc
    12781 ggggcaaatc attcatctcc ttgttgggta aggagtcttt cgtagaggtg ggtgttttct
    12841 tcatcataac agacaaaata tacctgttcg ggtaaagcgt gacgggtaat aaattctgaa
    12901 acggttttta ctgcgatttc agccgctgcc gcacgagggt aaccataaac cccagtactg
    12961 attgcaggaa atgccactga cgtatagctg tttgccgcca ccagtcgcag actattgaga
    13021 taggcatcct gcaaaagctg gtcttcgttt tgttcaccac cacgccagac tggcccgacg
    13081 gtgtgcacta cggctttagc gggaagatcg cctgcaagcg taataacggc atgccccgta
    13141 gggcaatcgc cctgctgttg cctgactttt aaacaggcat ccagcagagc cggacccgct
    13201 gcgcgatgaa tggccccatc gacgccgccg cctcccatta atgacggatt agccgcattc
    13261 acaatcacat caacggccag tttggtaata tcaccctgca caacatgaat acgcgttttc
    13321 ataacgcctc cttacacctt cttgtttctt aagtgtacag caggcttttt ggttaaatcg
    13381 catttttctg ctcagcaagg tgagttgttc agaaacttag ttatattcga tgttcattac
    13441 agccagcttt ttctgtttat caagatactg cacttttact gaggctattt gcgcgttttt
    13501 gacattgccg ctcattaacg ctttgctgga ataggttctg cccggaacag agccattctg
    13561 tgggcagttc atttctacgg gtgatgactg ggtgctgacg tcacatgctg gttccacaat
    13621 ttggccataa aaatggatca cgcccccttg cgcgctgctt cctgcgatgg caataaaagg
    13681 aaaaaacaaa ctgccgagca taagagagtt taaaaaaggt ctgaacattt attctatttc
    13741 ctcaatgatt agtcatcctt gagggttgtg ttatccatac tttcaggttg gctgatccgc
    13801 agcagaccat tctctccaga ttcatcttat gctcgatatt tcaacaaatt aagacttttc
    13861 tgaagagggc ggccattgtt gtgataaatg aagtgactgt ccatcagaaa cagtaacaac
    13921 tattttcacc cgatcgtccg gggaaatatt taaactcaac ttcgtcaaag caatgggttg
    13981 attagcaggc aatgagaggg tcttttcttg cttcgtctga ctttgccctg aactgccttc
    14041 gcgcagggac aatatttgta ctctgcacag acaagattga gtaagagtga cttcaggaat
    14101 aatggtgtac atatcccctt gctgggtcgt attaaaggtt atctgactgg aaagtgccgc
    14161 aaggagtaat aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt
    14221 ctttaataca gatgtgtatt agtactgatg agcggtcgcg ttgttaccaa agccaacctg
    14281 agtgacgtta acgctggagt tagatgcagt ctggtcaaca gcagcaccgt taccgccacc
    14341 gaactgttta acagtcatag tagaatcttt accattccac tgatcaagag tagcgctgtt
    14401 gccgaaacca cgctgagtca gatcgattga gctgtcatca gaaccttggc caacatctgc
    14461 gccgttaccg ccgccgtgct gggtaatggt cagatcagag ttacgggcgt cagcttgcag
    14521 agcaagtgca gagttaccac caccgtactg gtaaatgttc agctctgaat ttgggccgct
    14581 attattaccg ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag
    14641 agcgctaccg gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa
    14701 cccccatcgg attgatttaa aagtcgaata gtaattaacg ttgtgtcacg cgaattgcca
    14761 tttgcgactg tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg
    14821 ctttattacc agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa
    14881 tactggcatc gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct
    14941 ggtcaatctt tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc
    15001 ctccctgccg taactgagca ctattattag tcccagcttg accaattatg gctgcctgat
    15061 taaatgaaga cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat
    15121 aacctgctgc ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt
    15181 tgtttttcat gttgtcaccc tggacctggt cgtacactta agaaattaaa tcatttcaac
    15241 ttggttgtta acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc
    15301 ggaaatattt ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag
    15361 tattaaaacc cacgtaaata cgctgttatc tacgcaaaaa aatattttgt tttcttttaa
    15421 atctccgttt tccgcaatca aaaatcacca gacagccatt cttcttgccc gtcgctgatt
    15481 gctgcgatat gtcttgcgca caagccgtga caaacctcgt ctttttgcag taaaaaattg
    15541 tccacggagg tgtgaagaaa aaacaagaac gttttacatg acgaaaggac tacaccgaaa
    15601 tattttttat atgcattatt agtaagttag caccatttgt atgatttttt aaaattgtgc
    15661 aataaaaacc aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaagttta
    15721 acattttaat ataacgagtt acatttagtt acatgtttaa cacttgattt aagatttgta
    15781 atggctagat tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga
    15841 tttattacta cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag
    15901 gcagctgtca ggtgtgcgat caataaaaaa agcggggttt catcatgttt aatgaagtcc
    15961 atagtattca tggtcataca t
//
LOCUS       DAOTRK010000109        15835 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.109, whole genome shotgun sequence.
ACCESSION   DAOTRK010000109 DAOTRK010000000
VERSION     DAOTRK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.109"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            132..734
                     /locus_tag="QIE41_003336"
     CDS             132..734
                     /locus_tag="QIE41_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDS3532188.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(773..1198)
                     /gene="nudI"
                     /locus_tag="QIE41_003337"
     CDS             complement(773..1198)
                     /gene="nudI"
                     /locus_tag="QIE41_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDS3532189.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            1477..2019
                     /locus_tag="QIE41_003338"
     CDS             1477..2019
                     /locus_tag="QIE41_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532190.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2119..3321
                     /locus_tag="QIE41_003339"
     CDS             2119..3321
                     /locus_tag="QIE41_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDS3532191.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATARGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TSGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3541..4323
                     /locus_tag="QIE41_003340"
     CDS             3541..4323
                     /locus_tag="QIE41_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDS3532192.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4338..5543
                     /gene="rhmD"
                     /locus_tag="QIE41_003341"
     CDS             4338..5543
                     /gene="rhmD"
                     /locus_tag="QIE41_003341"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDS3532193.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIHKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5600..6889
                     /locus_tag="QIE41_003342"
     CDS             5600..6889
                     /locus_tag="QIE41_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532194.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKMGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6906..7709
                     /gene="yfaU"
                     /locus_tag="QIE41_003343"
     CDS             6906..7709
                     /gene="yfaU"
                     /locus_tag="QIE41_003343"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDS3532195.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7750..8652)
                     /locus_tag="QIE41_003344"
     CDS             complement(7750..8652)
                     /locus_tag="QIE41_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDS3532196.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMREQAIKIALRMLE
                     QGIDRDLVLAATQLSEADLAANNH"
     gene            complement(8845..10035)
                     /gene="glpC"
                     /locus_tag="QIE41_003345"
     CDS             complement(8845..10035)
                     /gene="glpC"
                     /locus_tag="QIE41_003345"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDS3532197.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10032..11291)
                     /gene="glpB"
                     /locus_tag="QIE41_003346"
     CDS             complement(10032..11291)
                     /gene="glpB"
                     /locus_tag="QIE41_003346"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDS3532198.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDSQAVTDIHHGLESLCQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11281..12909)
                     /gene="glpA"
                     /locus_tag="QIE41_003347"
     CDS             complement(11281..12909)
                     /gene="glpA"
                     /locus_tag="QIE41_003347"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDS3532199.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYS
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13182..14540
                     /gene="glpT"
                     /locus_tag="QIE41_003348"
     CDS             13182..14540
                     /gene="glpT"
                     /locus_tag="QIE41_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDS3532200.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            14545..15621
                     /gene="glpQ"
                     /locus_tag="QIE41_003349"
     CDS             14545..15621
                     /gene="glpQ"
                     /locus_tag="QIE41_003349"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDS3532201.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         3651 a         3884 c         4286 g         4014 t
ORIGIN      
        1 tggcaaaatt ttgaggcatt ttgccggcat cgcaggattt ttaatattac accaactgct
       61 aattatcgcc agaaatgtcg ctcaatttca cggtaattgt ctggttgcgc ttgtctatag
      121 gtggagttta cgtgttagct ttttgccgct cttcgttgaa gtcaaaaaaa tatttcatca
      181 ttttactggc gctcgctgca attgccggac tgggtactca tgccgcctgg agtagcaatg
      241 gtttgccacg tatcgacaat aaaacgctgg ccagactggc acagcagcat ccggttgtcg
      301 ttttgtttcg ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa
      361 caggtattac ggtcaaaggt acccaggatg cccgtgaact gggcaacgct tttagtgctg
      421 atatccctga tttcgatctt tattccagta ataccgtccg gaccattcag tcggcgacct
      481 ggttttcagc gggtaaaaag ctgacggtag ataaacgact tcttcagtgc ggtaatgaga
      541 tttatagtgc aattaaggac ttacaaagca aagcgcctga taaaaatatc gttattttca
      601 cccataatca ttgcctgaca tatattgcta aagataagcg tgatgcgaca tttaaacctg
      661 attatctgga tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg gatggggaat
      721 tcgttaacca ctaaattttc aatctgacag ccagtaatgg ctgtcatcat tattacagaa
      781 gacctttcaa acgtaacgtt tttcgggtgg cgacattcaa atcatagtgc gccagatctt
      841 caggttttac ccacgcgtag tcctgaaact cttcgtttat tttcacttct cggttggcag
      901 aaacgcagtc aaaaatcagg taaatcatat aaatctcttc cttgcgacca tctgcatacg
      961 tcttggtgcg aatatcatcg ctgaaggtcc acggcgtgat ttctgtcaaa agcagctgtt
     1021 ctcccagttc ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg ccaggctcca
     1081 cgccgccacc cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac
     1141 acagcaaata agcaccatca ttttgaatca aagggcatac aatagtccgt tgtcgcacag
     1201 gggtattcct tataaataaa aagaaaccat gagaatacgg taactcagaa atatgtgaaa
     1261 gtgacagtaa tctcttttgc gatgataact acatttatta ttatttttgt gtgtatcgat
     1321 aaagtgtgtt tattttttcg gcgtttaatt atttaagaag tatttagtta caataagtga
     1381 ctagatccct tttttcgctc attgaatcct ctgcgccagg cagtacaatc ccgcagcttt
     1441 gggaattttg attaattgct tgtgggaaag aggataatga aaaaaattgc gctagcaggt
     1501 ctggccggaa tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg
     1561 ggtaaagaat acaccaatat tggtgtcggt tttgggactg aatcgacggg cctggcgtta
     1621 agcggtaact ggacacataa cgacgacgac ggtgatgccg cgggcgtggg gctggggttg
     1681 aatctgcctc tcgggccatt aatggcgacc gttggcggaa aaggcgttta caccaacccg
     1741 aattacggtg atgaaggtta tgcagcggca gttggcggtg gtttgcagtg gcaaattggt
     1801 gacagcttcc gcttgtttgg tgagtattac tactctccgg attcgctctc cagcggtatt
     1861 caaagttatg aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagcatt
     1921 gaggcgggtt atcgctacct gaatctgtcg ggtaaagatg gtaaccgcga caacgccgtg
     1981 gctgacggcc cgtatgttgg ggttaacgcc agtttctgat cccttccggc gcggcattct
     2041 gccgcgccat tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa
     2101 caaaagcagg agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta
     2161 cacgggcaaa tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg
     2221 ttgccattat ctcgccgcaa tacggtgggg gataaccttg atgacttagt gaccattctt
     2281 cgcgaacgga gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg gccgaccagt
     2341 gatgatttaa gcgcactcgc cgctgcgaca gcaagaggtg aaggcctggt gttgcatgaa
     2401 gcctggctca aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc
     2461 aaccgtaaac aagcggagct acctgccagt gctgaattta tcaataaccc ggtaggcacc
     2521 gcctgtggtt ttgccgtgca gcttaatcgt tgcctgatgt tctttacccc cggcgtaccg
     2581 tcagaattta aggtgatggt cgagcacgaa atcctgccgc gtctgcgtga gcgtttttct
     2641 ttaccacagc cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg
     2701 gcacaaagcc tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcctca
     2761 atgcctatca tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa
     2821 ctgtggctgg acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga
     2881 ctgcccgcgc agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag
     2941 caattcacca gcggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg
     3001 tgtgaagtgg ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa
     3061 cggcgggcca accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat
     3121 ctcaactttg cgctagccac gccagatggc actttcgctc tgcgtgtgcg tttcagcact
     3181 acgcgctaca gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgctg
     3241 cgccgttggt taaacggcca ggacattgcc agtgagcatg gctggattga ggttgttgag
     3301 tccatgacct tatccgtctg attccgccac tctgtgccgg tttactgcac cggcacattc
     3361 cccctccggc aaaatttgtc caacctgttg tactcaataa gaaaaaatcc gtgcgctgat
     3421 tcacaaattt gtgcgtaatt tctccagtcc gctggacgag gtgcgggatt tgatgcgctt
     3481 cacaatttta ctccccctga gcaacgagac tgattccagt atctaagact ggagtccagt
     3541 atgttggaat caagcaaagt tcccgcccta acacgggcta ttgatattct caatttgatt
     3601 gcccgcattg gcccctgtag tgctgcgacg atcattgaca cattggggat ccctaaaagc
     3661 acggcctatt tgctgcttaa tgaactcagg cgtcagcgtt ttcttagcct tgatcaccag
     3721 gaaaattttt gtttgtggac caagctggtg gagctttccg gtcatgcgtt aagcaagatg
     3781 gatctccggg aactggctcg accgcgtttg acgcaactga tggataccac cgggctgtta
     3841 tgtcacctgg ggattattga taacggcagc gcttattaca ttttgaaagt ggagtcatca
     3901 gccactatca gcgtgcgttc ccatgaagga aaaagccttt cgctttatcg ctccggaata
     3961 ggtaaatgcc tgcttgcctg gcaacctgca gctgtacagc aaagtattat cgaagagctg
     4021 gtctgggagc agatgacacc caccaccatt acccatccac aacggctcta cgaggaactg
     4081 gcgcgtattc gccgtcaggg ctggagttat gacaacggcg aagattatgc tgatgtccgt
     4141 tgtgtcgctg cgccggtgtt taacgccaat aacgagctta ccgctgcgat ttcggtggtc
     4201 ggtacccgct tacaaatcaa tgaagaatac cgtgattatc tggcgggcaa ggccattgcc
     4261 tgcgccaggg atatttcacg tctgttggga tggaaaagtc cctttgactt acaggcctca
     4321 taacaatgga gaacattatg accctaccaa aaatcaaaca ggttcgcgcc tggtttactg
     4381 gcggtgcgac agcagaaaaa ggcgctggcg gcggtgatta tcacgaccag ggggcaaatc
     4441 actggattga tgatcatatt gccaccccga tgagtaaata ccgcgattac gagcagtcac
     4501 gccagtcatt tggcattaac gttcttggca cgttgattgt tgaagtcgaa gcagaaaacg
     4561 gccagaccgg attcgccgtt tcgacagccg gtgaaatggg ctgttttatt gtcgaaaaac
     4621 atcttaaccg tttcattgag ggtaaatgtg tcagtgatat caaactgatc cacgatcaaa
     4681 tgctcaatgc caccctgtat tactccggct ctggtggcct ggtgatgaac acgatttcct
     4741 gtgtcgatct ggctctgtgg gatctgttcg gcaaagtggt cgggcttccg gtttataaac
     4801 ttttaggcgg cgcagttcgt gatgagattc agttctacgc cacaggtgcg cgtccggatc
     4861 tggcaaaaga gatgggcttt atcggtggca aaatgccgac gcactggggg ccacatgatg
     4921 gcgatgcggg gatccacaaa gatgccgcta tggtcgcgga tatgcgtgaa aaatgcggtg
     4981 aggatttctg gttaatgctc gactgctgga tgagtcagga cgtgaactat gccaccaaac
     5041 tggcccacgc ttgcgcgccc tataacctga aatggatcga agagtgcctg ccgccacagc
     5101 agtatgaagg ttatcgcgaa ctgaaacgca acgcgccagt cggaatgatg gtcaccagcg
     5161 gtgagcatca cggcacactg caatctttcc gtacgctttc agaaaccggt atcgacatta
     5221 tgcagccgga tgttggctgg tgcggtggct taaccacgct ggtggaaatt gccgcaatcg
     5281 ccaaatccag agggcaactg gtggtgccgc acggttcgtc tgtttactct caccatgcgg
     5341 tgatcacctt caccaatacg ccattcagcg aattcctgat gaccagcccg gattgttcaa
     5401 cgatgcgtcc gcaatttgat ccgattctgc ttaatgagcc agtgccggtg aatggtcgta
     5461 ttcataaatc agtgcttgat aaacccggtt tcggcgtcga actcaatcgt gactgcaatt
     5521 tgaaacgccc ctacagccac taatcactcc gggcgttgct gtgacaacgg taacgcccac
     5581 atattctaag gagcttttta tgagcaccgc tttgcttgac gccgtggtga agaaaaaccg
     5641 agcgcgttta attccgttta tgttggcgct gtatgtgctg gcatttctcg accgttcgaa
     5701 tattggtttt gccaaacaaa cctaccagat tgataccggg ctgagtaatg aagcttatgc
     5761 gctgggagca ggcattttct ttgtggtata tgcgtttctg ggtgttccgg cgaatctttt
     5821 gatgcgcaaa atgggggcca gaacctggat tggtacgaca acactgctgt ggggatttct
     5881 ttcggcagcc atggcatggg ccgatactga agcgaaattt ctgatcgttc gtactctgct
     5941 tggtgctgcg gaggccgggt tcttccctgg tatgatttat ctcacctcgc aatggtttcc
     6001 gcagcgtaat cgcgccagca ttatggggtt gttctatatg ggcgcaccgc tggcgttaac
     6061 actgggatca ccgctttccg gcgcgctgct ggagatgcat ggatttatgg ggcatcccgg
     6121 ctggttctgg atgtttgtga ttgaaggatt gctggcagtt ggcgctgggg tattcacatt
     6181 cttttggctt gatgacacac cggagcaggc acgttttctg agtaaacaag aaaaaacgtt
     6241 gcttatcaat caactggcaa gtgaagaaca acagaaagtg acttctcgat tgagcgatgc
     6301 gctgcgtaat ggccgagtct ggcaactggc gattatctac ctgaccattc aggtggcggt
     6361 ttacggatta attttcttcc tgccgaccca ggttgctgca ttgctgggga caaaagtggg
     6421 ctttacggcg tcggtggtca ccgccattcc gtgggtcgcg gccttgtttg ggacctggct
     6481 tattccgcgc tattccgata aaaccggcga acggcgtaat gtcgcggcgc tgacattact
     6541 ggcggcgggc attggtattg gtctctccgg gctgctttct ccagtactgg cgatcgtagc
     6601 gctgtgtgtt gcagccatcg ggtttattgc cgtgcagccg gtgttctgga cgatgccgac
     6661 acaactgctt tccggtacgg cgctggcggc ggggattggt tttgtaaacc tgtttggtgc
     6721 agtgggcggg tttattgccc cgatcctgcg cgtgaaagca gaaacgttgt ttgccagcga
     6781 tgcggcggga ttactgacgc tggcagcggt ggcggtcatc ggttcgctga ttattttcac
     6841 tctgcgtgta aatcgcactg ttgcgcagac cgacgtggca catcattaat aggttaagga
     6901 acaccatgaa cgcattatta accaatccct ttaaagaacg tttacgcaaa ggcgaagtgc
     6961 aaattggtct gtggttaagc tcgactactt cttatatggc agaaattgcc gccacctccg
     7021 gttatgactg gttgctgatt gacggggagc acgcgccaaa caccattcag gatctttatc
     7081 accagctaca ggcggtagcg ccctatgcca gccaacccgt gatccgtccg gtggaaggca
     7141 gtaagtcgct gattaaacaa gtcctggata ttggcgcgca aactctacta atcccgatgg
     7201 tcgatactgc cgatcaggca cgtcaggtgg tgtctgccac gcgctatcct ccctacggtg
     7261 agcgtggtgt cggggccagt gtggcacggg ctgcgcgctg gggacgcatt gagaattaca
     7321 tggcgcaagt caacgattcg ctttgtctgt tggtgcaggt ggaaagtaaa acagcactgg
     7381 ataacctgga cgaaatcctc gacgtcgaag ggattgacgg cgtgtttatt ggacctgcgg
     7441 atctttctgc ctcgttgggc tacccggata acgccgggca cccggaagtg cagcgaatta
     7501 ttgaaaccag cattcggcgg atccgtgctg cgggtaaagc ggctggtttt ctggctgtgg
     7561 ctcctgatat ggcgcagcaa tgcctggcgt ggggagcgaa ctttgtcgct gttggtgttg
     7621 acacgatgct ctacagcgat gccctggatc aacgactggc gatgtttaaa tcaggcaaaa
     7681 atgggccacg cataaaaggt agttattgat atcaaaggcc catggggatc ggctgtgggc
     7741 ctgtgttaat tagtggttat tcgctgccag atcggcttcg cttagctggg tggccgcgag
     7801 caccaggtca cgatcaatgc cctgttccag catgcgcaag gcaattttaa tggcttgttc
     7861 acgcatacct tcctgccagc caatttggtg tccttcctgc cgtagccgtt cagcaatagt
     7921 cattaatctc tccttttgta gtggtgaacg ttcggcaatc tcctcaataa aacgggtgaa
     7981 acgggaggta tcgccgcatt gcagcagata attaaacagc gtttgtagct ggctgtcatt
     8041 agtgaagcct ctaaccaaaa gcgtggtgat cctgtcgacc ataccgatta aatcgcggtc
     8101 gcgaatatgc ttttgaatca gttcaagcag agctatacgc cgatgttgca taatctcatc
     8161 gtcaggtacg atggtgatat ccaccaacgg aaaagcttcg gtgtacaact gccgggcaag
     8221 ttgcggatcg tcaaactcat ccagccagtt gagtgagtac gggtagggcg aggtttcgcc
     8281 atgataaaac agcaatggca ccaccagcgg aactctgtca tagcctttat ccaggtgacg
     8341 ctgcatggcg gcagtggcat agcgcattag ccgaaaagcc atattctttt ctgcagagct
     8401 ttgatgttca atcacgcagt agatataacc gtcaccgtcg ctggtttcca cggaccacaa
     8461 aacatccgag tagtaagcgc gtaaactttt ttcaataaaa ctggtgggtt ccaggcgtaa
     8521 ggtttgcagg ttgcaaagct tgcgcagcgg ctctggtaga tgtatttcca gaaaatcccg
     8581 tgcggtttcg ggtgtgaaca taaaggtttt aaacaccgca tcatgcgggg aggaggttgt
     8641 tgattcggtc atggttgtcc gtcacctgat atttatgatg acgcgacatt acggttatcc
     8701 caggcctttg acatctgcca gttttagcct ttgcgagacg ttttcagagg aattttcact
     8761 gcgttgcaac gcatacattg ggcacggaat cgtgtacgca aaaatgcccg gtaacaacat
     8821 gcttaccggg catcagaaag gagtttaagc cagcgcctgg gccagcagcg taatcggatg
     8881 ttcgcagcga agacttgtgg acatctcaat ctgccatttg caggtttcgc agtcggtaac
     8941 caccagatct gcgccgcttt cttctatctg acggaacagt ggtgcgccga tggcttgtga
     9001 ggtggggtag ttctcttttt tgaaaccgta agtacctgca ataccgcagc actgggaatc
     9061 cagcaccgtt aactcaagtc ccgggatttt acgcaacagc tccagggtgt agagcgtcca
     9121 gcccattttt tccatatggc acggagtgtg ataaaccact ttcagcggca gcggtttcag
     9181 cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag cgggtcgcca gttcgatgtg
     9241 atcgcgcaag cctttgttgt cgacattcag cacttctggg tattcgtcgc gcagggcaaa
     9301 tgtacaggtt gaggaggtgg caatcaccgg aatgcctttt actcccactg cttcgcggat
     9361 cgactctaca tttgtgattg cctgtttacg tgctttatcg gtaaagccgt tggcgatcag
     9421 cggtacgccg cagcattttt ctttgctgag cagttgtaca ccggtaccca ttgcgttgag
     9481 cactttaatt aaatctttgc ctaactgcgg atggttgtag ttaacgaagc agccgtgaaa
     9541 gaaagccacc tggtctttat attgcgcttg ttgggctgcc acgctgcgat accagcgacg
     9601 gaacgtaccg aaggagtatt tcggtagcgt gcggcgatga tcgattttta acgccgcatc
     9661 aagcagctgc cgcactggtt tcagcgaggt agcggtattg acgattggtg cgaatggcgt
     9721 ggaaacgcta cccatcaggt cggtatgact caacacaaaa ttgcgcagcg acgggcgtgt
     9781 ggtgtcatat ttcgcccgtg cgcgctggat gatatcgcca atcttcacat cggacgggca
     9841 ggcgacttcg caacgtttgc agttgatgca atatttcagt gcctcgtcat acagtgcgcc
     9901 atccttcaaa cgcagacgct cgccatccgg cccggcttgt tttggccccg gatatcccgg
     9961 attcacccgg ctcaccgggc aggcggtggt gcagacggtg cacttaatgc agttttcgaa
    10021 gctggtgtcg ttcattgttg acctcctgcg cgttgggcaa tctgttgagc ggcatgtaaa
    10081 gcactgacgg cacaaacacc gccgccgcat ccctgggcga tgggatcaaa tccgcccagc
    10141 accgaaccga tggcaaacag gttctcaatg gtttgccctg cctgcgacgg gcgtagtgcc
    10201 tcatcagtgg ttacaccgaa cagctgccac ggttgcggcg caaaaaaatc gcccttatac
    10261 cattcacccc gcgtggcggt ttgtagcaca tcaaggccga gaatcggctc gcgaatgccg
    10321 tcacgttctg ccaccagtcc gccgctaaag aaactgccgc tcgcaagcac cgcaaatcgt
    10381 gggcgcagcg ggatatcggc gtgattgcgc gtccagattt cattcactac gccattttta
    10441 caggtcactt ttttcacttc atcgcctggc atccacacgc caccctggcg cacaaactgg
    10501 cgctgtaact ggttttgcag acgaatgccc agcacggaag gcggcagcgt tggcaaaagc
    10561 atcagtgaac aaggtagttt ttcattcaac caacgccaca gtttgtcatc ggccaggccg
    10621 aagcaggcgg gcatcaggat catttcgcag gtattggcga caggaataag cgcatcaagt
    10681 aacagcggcc agttttcttc attatcaagg aaacgggcga tattcaccgc gcggaactcg
    10741 gtggcgttat tgcgcaggac atccagttcc ggcagctcta tttctgcggt ttcaacggca
    10801 aggccgagtt cacgcaacga agctgccgca aggtgagcct gaaaatccat caggccgcta
    10861 attcccacta cacatatttt cttcgcgggc agtggccaga cggggacttc tggcgaactt
    10921 agccaggtag aacgcagagt gccgagcggc gtaatccgct ggtgagccag ttctacgctg
    10981 ccctgcaatt gcgcaccgct ttcagcgatt aatgcctgcg cctggcaagc gagatcgagt
    11041 acgcgttgtg gcccgagaag agagtaagga tgggcagggg cctgctgaca taacgattcc
    11101 agcccgtggt gaatgtctgt cacagcttga ctatctggca gatggctcag caaatccagc
    11161 gaaccggatg agaaatgcag cgcgctttga ccacgcgtga caatggcaca gcgcaggccg
    11221 tgtttttgca gttgcaggcc acagagtaag ccggcgaggc cgccgcccat aatgacagta
    11281 tcaaagcgca tctttctgct ccttctccag accacacagt ccctgataaa cccagcgggt
    11341 aaattcgctt tcgcgcagtg cgtctcccca tgctatgggt tgcacgcctt tccagcgttc
    11401 gttaaggaag gtggaaagtt gctcgataga ttgcgcggac gtcgtgacgt taaaacgttg
    11461 cagcagtccg gcagcccggc aggcgcagag ttcgccctgg caggtaccca tccctacacg
    11521 ggtacgacgg cgtaaatcca gcaggctgtt aacgtttaaa ttttctactg cgtactgcac
    11581 ttcacccgca gttaccgctt cgcactcaca taccaggctg cggtgcaggc ggccttcgct
    11641 cagccaggct ggtgtgcgat cgccgtgccg gtaaactgcg gaaccgcgca gcggagcggg
    11701 cagggagatc actttgcgca gcgtgacttc tgccggttcc tgtgagccag gcaacggcgt
    11761 gtcggcagtg gtacaggggc gcgtgttgcc cagcttgcga cacaccgcgt cagtagccca
    11821 ttccgccatc agccgatagg tcatcagttt gccaccggtg atggtgataa atccgtcgag
    11881 accatcgcgt tcagcatggt cgagcagcac gatgccacgg ctgacgttac gtccgctcgg
    11941 gtcgtcatcg ctggcaacca gcggacgcac gccagaatag gcccgcaaaa tgcgtgtttt
    12001 cgccatcacg ggggccagtt tttccccttc acgcagcaga atatcaactt cttctgccgt
    12061 cactcgatta tcgtcaatct cgctgtaatc aatacgtaaa gaggtggtgc caatcagcga
    12121 aatggtatcg ccaggcacca gaatatcggc gtcggaaggt ttgcggcagc ggttgatcac
    12181 atgctgatta atgcggtgat ccataatcag cagcgatcct ttcgccggga acatgcgaat
    12241 gcgcagatcg gcatattcgg caatgtgttg cccccaaatc ccggcggcat taactacgac
    12301 aggcgcatga agtgcctgaa tttcgccggt gagatggtta cgtacacgaa caccgcacac
    12361 cgtcgcgcct tcacgaatca gccccgtgac ttcatgagcg gtaaggataa ccgcaccgtg
    12421 ttctttggca tcgagcatgt tcgctgcggt cagacgaaat ggatcaacgg tgccatccgg
    12481 aactttcacc gcgccaatca gtgccgggtt aacggcaggt tcgataatgc gcgcttgctg
    12541 cgggtctata gcttctgcgc tgatccctgc ttcttcgcag gcgcgaataa aagtggcctg
    12601 gaaggagagg tcatcttccg gcagggtgat aaacaggccg ttggtcggtt caacgcagtg
    12661 acgtgcaatg cgtttcagga tctggttttc actaatacat tcgcgggccg attccgcatc
    12721 ggttaccgca tagcgcgcac cactgtgcag caggccgtgg ttacgcccgg tggcaccggt
    12781 tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg cgcagggcac agtcgcgggc
    12841 aatcccggct cccgttgcgc cgccgccaat gataatcacg tcacttgatt gcgagtcgcg
    12901 agttttcatt gttatccctc tgaagttcgt tttttaccat ttagccatag taaaaacatg
    12961 aattgtttga tttcgcgcat attcgctcat aattcgaaag tgaaaagtga tttcatgcgt
    13021 cattctgaac atttcaacca tcgtatttaa taatgtgtgc ggtaattcac atttaattta
    13081 tgaatgtttt cttaacatcg cggcaactca agaaacggca ggttctccca ctgaatcagg
    13141 ctgttaatca taaataagac cacgggccac ggaggctatc aatgttgagt atttttaaac
    13201 cagcgccaca taaagcgcgc ttacctgccg cggagatcga tccgacttat cgtcgattgc
    13261 gctggcaaat tttcctgggg atattctttg gctatgctgc ttactatttg gttcgtaaga
    13321 actttgcgct cgctatgcct tatctggttg agcagggatt ctcacgcggt gatttaggtt
    13381 ttgccctttc ggggatctcg attgcttatg gattttcgaa attcatcatg ggttcggtat
    13441 cggatcgctc gaatccgcgc gttttcctgc ccgcaggttt gattctggcg gcggcagtga
    13501 tgttgtttat gggctttgtg ccatgggcga cgtcgagcat tgcggtgatg tttgtgctgc
    13561 tgttcctctg cggttggttc caggggatgg gatggccgcc gtgcggtcgt actatggtgc
    13621 actggtggtc gcagaaagaa cgtggcggca ttgtgtcagt gtggaactgt gcgcacaacg
    13681 tcggtggtgg tattccgccg ctgctgttcc tgctggggat ggcctggttc aatgactggc
    13741 acgcggcgct ctatatgccc gctttctgcg ccattctggt ggcactgttc gcctttgcga
    13801 tgatgcgcga taccccgcaa tcctgtggcc tgccgccgat cgaagagtac aaaaatgatt
    13861 atccggacga ctataacgaa aaagcggaac aggagctgac ggcgaagcaa atcttcatgc
    13921 agtacgtgct gccgaacaaa ctgctgtggt acatcgctat cgccaacgtg ttcgtttatc
    13981 tgctgcgtta cggcatcctc gactggtcac cgacttatct gaaagaggtg aaacacttcg
    14041 cgctggataa atcctcctgg gcctacttct tctatgagta tgcgggcatt ccgggcaccc
    14101 tgctgtgcgg ctggatgtcg gataaagtct tccgtggcaa ccgtggggcc accggcgtct
    14161 tctttatgac actggtgacc atcgcgacta tcgtttactg gatgaacccg gcaggtaacc
    14221 caaccgtaga tatgatttgt atgattgtta tcggcttcct gatctacggc cctgtgatgc
    14281 tgatcggtct gcatgcgctg gaactggcac cgaaaaaagc ggcaggtacg gcagcgggct
    14341 ttaccgggct gtttggttac ctgggcggtt cggtggcggc gagcgcgatt gttggctaca
    14401 ccgtggactt cttcggctgg gatggcggct ttatggtaat gattggcggc agcattctcg
    14461 cggttatctt gttgattgtt gtgatgattg gcgaaaaacg tcgccatcag gaattactgc
    14521 aaaaacgcaa cggaggctaa tggcatgaaa ttgacgctaa aaaaccttag catggcgatc
    14581 atgatgagtg gaatgatcat gggaagcagt gcaatggcgg cggatagcaa cgaaaaaata
    14641 gtcatcgccc atcgcggtgc cagtggatat ttgccggagc atacgctgcc agcaaaagcg
    14701 atggcgtatg cgcagggagc ggattatctg gaacaggatt tggtgatgac gaaggacgac
    14761 catctggttg tcctgcatga ccattatctc gatcgtgtta ctgatgttgc cgatcgtttc
    14821 ccggatcggg cgcgcaaaga cggtcgttac tacgcgatag atttcacgct cgatgaaatt
    14881 aagtcgctga aatttaccga aggtttcgat attgaaaacg gtaagaaagt acagacttat
    14941 ccggggcgtt tcccgatggg taaatctgac ttccgggtac acactttcga agaagagatt
    15001 gaatttgttc aggggttaaa tcactctacc gggaaaaata tcggtatcta tccagaaatt
    15061 aaagcgccgt ggttccatca tcaggaaggg aaggatattg cggctaaaac gctggaagtg
    15121 ctgaagaaat atggttacag cggtaaggac gacaaagttt atttgcaatg ttttgatgcc
    15181 gatgaactga agcgtattaa gaatgagctg gaacctaaaa tgggcatgga cctcaatctg
    15241 gtacagctga ttgcctatac cgactggaac gaaactcagc agaaacagcc ggacggaagc
    15301 tgggttaatt acaactacga ctggatgttt aagccggggg ccatgaaaca ggtggcggaa
    15361 tatgctgatg gtattggtcc ggattatcat atgttgattg aggagacatc gcagccaggt
    15421 aatatcaaac tcactggcat ggtgcaagat gctcagcaga acaagctggt agtgcatcct
    15481 tacaccgtgc ggtcagataa gctgcctgaa tacactactg atgtgaatca gttatatgat
    15541 gctctgtata acaaagcggg tgtaaatggg ctgtttactg atttccctga taaggcagta
    15601 aaattcctta ataaagagta atgctattgc aggatggcgt tcatgcgcca tcctctgcta
    15661 aagcgaaata atgctactcc cggttgttct attcattaac cggtgcatcc tgttaaaaaa
    15721 gatgccaaca tatttgcgta aaactcattc gttaagaaag ctaacactgg gcattgttat
    15781 tccttaaaca gtatgaattg ggttcaataa taaattgtat tgcagtttat actgt
//
LOCUS       DAOTRK010000110        15757 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.110, whole genome shotgun sequence.
ACCESSION   DAOTRK010000110 DAOTRK010000000
VERSION     DAOTRK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.110"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            39..929
                     /locus_tag="QIE41_003350"
     CDS             39..929
                     /locus_tag="QIE41_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDS3532202.1"
                     /translation="MNKKYELVVKGINNYGDKVTVTVKPEGDGQASLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYKGKLPG
                     RITCSLKMPPSTLRSEKDDVESRIEKLESYVVELNKKWSILVPSGDEKQFAAFDDYCR
                     KVMSRNLAECFSIHNDNFSDPEWECNRPSFVVSGDAGKITISENGKVTPPSHQHSEEL
                     IEFAIDYLKNNKKQGLMKRVGRCMGYLQVAAEIEALASGADKDAIVREALLRDFNTPP
                     FKKVPAYWLHPGLTYLKVRI"
     gene            966..1499
                     /locus_tag="QIE41_003351"
     CDS             966..1499
                     /locus_tag="QIE41_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDS3532203.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGKHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1656..1838
                     /locus_tag="QIE41_003352"
     CDS             1656..1838
                     /locus_tag="QIE41_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532204.1"
                     /translation="MAKCKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1853..1984
                     /locus_tag="QIE41_003353"
     CDS             1853..1984
                     /locus_tag="QIE41_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532205.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVALFLSMAWMMLSSSGLLG"
     gene            1987..2454
                     /locus_tag="QIE41_003354"
     CDS             1987..2454
                     /locus_tag="QIE41_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDS3532206.1"
                     /translation="MNRMLSAFIVILLVACGALWMATDHYRDNAITYKAQRDNKASEL
                     KLANATITDMQVRQRDVAALDAKYSRELADARTENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPQLADTVTRDYFTLRERLMTMHKQLEGAQDYIRTQCLK"
     gene            2766..3092
                     /locus_tag="QIE41_003355"
     CDS             2766..3092
                     /locus_tag="QIE41_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HDS3532207.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            complement(3215..3568)
                     /locus_tag="QIE41_003356"
     CDS             complement(3215..3568)
                     /locus_tag="QIE41_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532208.1"
                     /translation="MPHTVHYPEQPSQGHVTPPPSRDAPVRFVLCCVAKTDNIRIYLV
                     LHDEFTQRLIEEGKMVSKSKAHCRRMLQALQQTRAGIFDQLENCQHTLPEYIAISSET
                     SATLIHRVPPEKKKK"
     gene            4051..4560
                     /locus_tag="QIE41_003357"
     CDS             4051..4560
                     /locus_tag="QIE41_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDS3532209.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            4532..6460
                     /locus_tag="QIE41_003358"
     CDS             4532..6460
                     /locus_tag="QIE41_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDS3532210.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAADNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKAEPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            6444..6650
                     /locus_tag="QIE41_003359"
     CDS             6444..6650
                     /locus_tag="QIE41_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3532211.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAELVLGTTRRRRRPLGVRL"
     gene            6647..8239
                     /locus_tag="QIE41_003360"
     CDS             6647..8239
                     /locus_tag="QIE41_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDS3532212.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQEQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            8229..9734
                     /locus_tag="QIE41_003361"
     CDS             8229..9734
                     /locus_tag="QIE41_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDS3532213.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGILLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAVQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFEHFMANHSPAAVQGGVSQASEDGDADVKMLMAMP"
     gene            9771..10118
                     /locus_tag="QIE41_003362"
     CDS             9771..10118
                     /locus_tag="QIE41_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDS3532214.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     GATGKLVVWDGQKAGSAVGILVLPLEGTEAVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFTGSALSHAALP"
     gene            10176..11204
                     /locus_tag="QIE41_003363"
     CDS             10176..11204
                     /locus_tag="QIE41_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDS3532215.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPIVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVELLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            11256..11639
                     /locus_tag="QIE41_003364"
     CDS             11256..11639
                     /locus_tag="QIE41_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDS3532216.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVMVPSPLATAMIQHGTAEHA"
     gene            11632..11985
                     /locus_tag="QIE41_003365"
     CDS             11632..11985
                     /locus_tag="QIE41_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3532217.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQRGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            12001..12534
                     /locus_tag="QIE41_003366"
     CDS             12001..12534
                     /locus_tag="QIE41_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3532218.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRCERLP
                     GELASALKQQLRIAIKR"
     gene            12531..12926
                     /locus_tag="QIE41_003367"
     CDS             12531..12926
                     /locus_tag="QIE41_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3532219.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            12934..13680
                     /locus_tag="QIE41_003368"
     CDS             12934..13680
                     /locus_tag="QIE41_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3532220.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDGWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTGTVEKGKTTTLTVTVEPENATDKTFRAISADPSKATISVKDMTITVTGV
                     KDGKVSIPVISGNGQFAAVAEITVNNVPGG"
     gene            13699..14130
                     /locus_tag="QIE41_003369"
     CDS             13699..14130
                     /locus_tag="QIE41_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDS3532221.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            14157..14570
                     /locus_tag="QIE41_003370"
     CDS             14157..14570
                     /locus_tag="QIE41_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDS3532222.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            14551..>15757
                     /locus_tag="QIE41_003371"
     CDS             14551..>15757
                     /locus_tag="QIE41_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDS3532223.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAAMVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMAKQFHNVTAEQIAYVAQLQRSGDETGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQ"
BASE COUNT         3774 a         3624 c         4794 g         3565 t
ORIGIN      
        1 agaggaccgg cgtaaggcgg cgcggggaga gtaaagtgat gaataaaaaa tatgaactgg
       61 ttgttaaggg gataaataat tacggggata aggttactgt tactgtgaag ccggaaggtg
      121 acgggcaagc gtcgctgttg ttgccagatg tggcgattag tcttgaccgt actgaaggtg
      181 ccacgctgga gttttacgaa gctgaggcga aaaagcaggc gaagcagttt ttcatggatg
      241 ttgctgccgg gttatgtgaa ggggatggtc cgttgccgga aaagcgcccc gtaattttag
      301 aggcgcagga tgtgttgata acctacaaag gaaagctacc gggaagaatt acttgttctc
      361 tgaagatgcc gccgtcaaca ctgcggtcag aaaaagatga tgttgaatca cgtattgaaa
      421 aactggagag ctacgtcgtt gagctgaata agaaatggtc gatattggtg ccttctggcg
      481 atgaaaagca gtttgctgcg tttgacgatt attgtcggaa agtgatgagc agaaatctcg
      541 cagagtgttt cagtattcat aatgataatt tcagtgaccc ggaatgggag tgtaaccggc
      601 catcctttgt tgtatccggt gatgctggga aaataaccat ctcagaaaat gggaaagtaa
      661 cacctccatc gcaccagcac agtgaggagc tcattgaatt tgccattgat tacctgaaga
      721 acaataaaaa gcaggggctg atgaagcgcg ttggccgttg catgggatat ctgcaggtag
      781 ctgctgagat tgaagcgctg gccagtggtg ctgataagga tgcaattgtg cgggaggctc
      841 ttcttcgtga ttttaatact ccacccttta aaaaagtgcc ggcttactgg cttcatccgg
      901 ggctgactta tcttaaagtg cgtatttagt gggccaggga cagcggctga atatttaata
      961 tatccatgaa caccaaaatc aaatacggcc tgtcggctgc cgttctggcg ctgattgccg
     1021 ctggtgcgcc tgcgcctgac attctcgacc agtttctgga tgaaaaggaa ggcaagcaca
     1081 ccacagcata ccgtgatggt gcaggtatct ggaccatctg ccgtggtgcc atcatggtgg
     1141 atggcaaacc tgtcgttccg ggcatgaagt tgtcgaagga aaaatgcgac caggttaacg
     1201 ccattgagcg tgataaagcg ctggcgtggg tggagaaaaa catcagagtg ccgctgaccg
     1261 aaccccagaa agcggggatc gcgtcattct gtccgtacaa cattggtccc ggtaagtgtt
     1321 tcccgtcgac gttttacaga cgaattaatg ctggtgatcg aaaaggtgcc tgcgaagcga
     1381 ttcgctggtg gattaaggac ggtggcagag actgccgtat tcgttcaaac aactgttacg
     1441 gtcaggtatc ccgtcgtgac caggagagcg cgctggcgtg ctggggtatc gacagataag
     1501 cagaatattt tgctgaaaaa tgaggaatgg ccacgcgggc ggataacacg aaatcctgcg
     1561 aactggcgaa acgtaagtga ataaaagtaa aaaccccgtt tgttggcacc aagcgaggtt
     1621 ttgtgtttct gaccttgagt aaggcaaggg agaacatggc gaagtgtaaa cgaattctgt
     1681 tgaggttgac tatgaaaaat ggccttgaac tgaaagcgcc tgtaactgat gacatcagca
     1741 gagcactggc ttttgccatt aagtgggtgg cggtcggtgt tgctgtgtcc ccgatgctgt
     1801 atgggctggc aaaactggtc attgcgttga aatcgtgaag ggaggattaa gcatgtcaga
     1861 caaactcata acgctggcga agatcctctg tgtaattgtc ggcatttcat tttcactaat
     1921 gctggttgct ctttttcttt ccatggcctg gatgatgttg tcttcgtcgg ggttgctggg
     1981 gtgaacataa accgaatgct ttccgcgttt atcgttattc tgctggtggc ctgtggtgcg
     2041 ctgtggatgg caacagacca ttaccgtgat aacgcgatta cctacaaagc gcagcgcgat
     2101 aacaaagcca gtgaactgaa gctggcgaac gcaaccatta ctgatatgca ggtgcgccag
     2161 cgcgatgttg ctgcgctcga tgcaaaatac tcgagggaat tagccgatgc gagaactgaa
     2221 aatgaaactc ttcgcgctga cgttgccgct ggtcgtaagc gcctgcggat caacgccacc
     2281 tgtccaggct ccgtgcgtga agcccccacc acctccggcg tggataatgc aaccggcccc
     2341 caactggcag acaccgttac acgggattat ttcaccctca gagagcggct gatgacgatg
     2401 cacaagcaac tggaaggggc acaggactat atccgcactc agtgcctgaa ataagttttg
     2461 ttgatgcgcc gtatcgtcgc tatattccct cattaacaga gaccgcagcc cgacagggag
     2521 actcctctgc gcgagtgtgc ggggataatt aaaaacgatg cacaccgggt tttttaccgc
     2581 gttaatgatt cgcgggttta tcccggtgcg atggtggaag aaacaggaag ctgtattaca
     2641 gaaagtgcta ctactgtatc ccgatgcgat gtatgtaatg tgagtcagat aatggcacag
     2701 gatgtggtga tgtggcagtc tggaacacag gatatattgt cagaataaga cccgtaggaa
     2761 taaaaatgaa aagacgcctt ttactacttt ttctgttatc tgtcctggca gtgggatgct
     2821 cgcagcaaaa agctgatgag ccccggcaat tagtgacggt gtatccacga tatccggaat
     2881 atgctgcagc aaattatatc aaggggctgg ttgaggttaa gttcgatatt ggtgctgatg
     2941 ggactgtgac acggatcgtt tttctccgct cagagcctca taatttgttt cgtgatgaag
     3001 tggtgaaggc catggcgaaa tggcgatttg aaaagaatcg cccctgtcag ggagtgaaga
     3061 gacaatttat ctttacgccg tcacgtcctt gatgcttcca ggtagagagg ggctggacgc
     3121 aggagaaaaa tgaaagagcc agcggttata tttttgtcat ggctgacgag gaatgatgga
     3181 agaaggcgtt gtatgccaca caacgcctca ctgttcattt cttctttttc tctggtggaa
     3241 cccgatgaat aagagttgca ctggtttccg atgagatggc gatatactcg ggcaaagtat
     3301 gctggcagtt ttccaactgg tcaaaaatac ctgctctcgt ctgttgcaat gcctgcagca
     3361 tgcggcggca atgcgccttg cttttactaa ccatctttcc ttcctctatc agtcgctgcg
     3421 tgaactcatc atgtaatacc aggtaaatgc ggatgttatc ggttttggct acgcagcata
     3481 gtacaaaacg gacaggtgca tcccgggacg ggggaggcgt cacatgtccc tgtgatggtt
     3541 gttccgggta atgcactgtg tggggcataa aaatgtccga taattttact ttctaccgca
     3601 gttagttgat tcgttggtcc tggtagcaca ttgggcgagg atttaaatgc caggcaactg
     3661 aaggatgatg ttgcaaggga gatagcgaga atatttctga ttttcatttg atgatgcctc
     3721 tgtgtgaaat gacggtaaac gacgcacttg tgccggcaca taatagcaag caccataata
     3781 gatcagattc gattcttgct gtaagtgata attattctcg ttttcgggtc ctttccgtcg
     3841 atccaacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg
     3901 gaaccatgtc cggtttctct tcaagttaac tatatgaaaa ataaaaaaag aggtcttctg
     3961 tgaaccgggc atgcacaaaa aatagacatg taagccggac atgaccggtt ttgttgtgat
     4021 tgtgaagtga gagtttttgc gaggtgagga gtggctacgc agactgaagt tgccaggcat
     4081 ttaagtctga ccgatcgcca gcttcgcaga ttgcagaaat tgccgggtgc cccgatatcg
     4141 aataagcgag ggcaactgga tctggatgcc tggcgcgatt tttacatatc gtatctgaga
     4201 agaagtaaaa acgatgtgcc tgatggcgat agcgaagacg actatgagga gaaattgctt
     4261 attgccagat gggaactgac agcagaacag gctgttacac agcagttaaa aaatgaggtg
     4321 tcaaaaggaa aacttattga taccgggttc tgtatttttg ctctcagcaa gctggcaatg
     4381 gcgttatcca gtacgcttga ttccatccct ttatccatgc agcgacagtt tcctgattta
     4441 acaccgcgcc atcttgacca tctgaaaacc cttattgcga agggggcaaa tcagtgtgcg
     4501 cgggcggggg ataaattacc ggatttactc gatgaatata tcagagcaac aactgaataa
     4561 tatgatgagt gctgtcacaa cagcattaca gcccctgata agggcattgc cggtgacgcc
     4621 agttgaatgg gctgatcaaa attattatct gcctaaagaa tcttcatatg gtgagggaga
     4681 atggaaaacg ctgccgttcc agatcgccat catgaacagc atggggaatg atcagatccg
     4741 gactgttaat ctgattaaat ctgcccgtgt tggctataca aagatgttgc tgggggtggt
     4801 cgggtatttt attgagcata aatcccgaaa cagtctgctt tttcagccca cggattctgc
     4861 cgctgaagat tttatgaagt ctcacgtgga ggcgacgatt cgggatgttc cctgcctgaa
     4921 aaaactttct ccatggctgg ggcgtaaaca tcgtgacaat actctcacgc tgaaacgctt
     4981 ttcatcgggt gtgggcttct ggtgcctggg cggcgctgcc gccaaaaact accgtgaaaa
     5041 atccgtggac gtggtctgct atgacgaact ttcctcgttc gaaccggatg tcgaaaaaga
     5101 gggttcgcca accctgcttg gggataaacg tattgagggc tctgtatggc ccaaatccat
     5161 tcgcggctcg acgcctaaaa tcaaaggcac ctgccagatc gaaaaagcgg ccaacgagtc
     5221 ggcgcatttc atgcgttttt atgtgccctg cccacactgt ggggaggagc agtatctgaa
     5281 atttggcgat gaatccacgc cttttggcct taaatgggag aaggacagcc ccgaaagcgt
     5341 tttctacctc tgtgaacatc atggctgcgt gatccatcag tctgagcttg accagagcaa
     5401 cgggcggtgg atctgtgaaa acacggggat gtggacccgt gacggtctga cgtttttcag
     5461 cgccgcggat aatgaaattc cgccgccgcg ctccatcacg ttccatatct ggacagcgta
     5521 cagtccattc accacctggg tacagatagt ctatgactgg ctggatgcac tgaaagatcc
     5581 caacggcctg aaaacctttg tgaacaccac gctgggcgag acctgggaag aggccgtggg
     5641 cgaaaaactc gatcaccagg tactgatgga taaggtcgtg cattacacgg cggcggtgcc
     5701 tgcccgggtg gtttatctga cggcgggcat tgactcgcag cgaaaccgtt ttgagatgta
     5761 tgtctgggga tgggcaccgg gagaggaagc ttttctggtg gataaaatca tcattatggg
     5821 gcgtcctgat gaggaagaga cgctgttacg tgtggatgcg gcgatcaaca aaaaataccg
     5881 ccatgcggat ggcaccgaaa tgaccatttc ccgtgtctgc tgggacaccg gggggatcga
     5941 tggtgaaatc gtctaccaga ggtcaaaaaa acacggtgtt ttccgtgtgc tgccggtaaa
     6001 aggcgcgtct gtctatggca agccggtgat caccatgccg aaaacccgca atcagcgggg
     6061 cgtttatctg tgtgaagtgg ggacggacac cgcaaaagaa attctctatg cccgtatgaa
     6121 agccgagccc acgcctgcgg atgaagccac gtcgtatgcc atccgttttc ctgatgatcc
     6181 ggagattttt tcgcagacag aggcgcagca actggtcgcg gaagagcttg tggagaagtg
     6241 ggaaaaagga aagatgcgtc tgctgtggga taacaaaaag cggcgtaacg aagcgctgga
     6301 ctgcctggtg tatgcctacg cggcattacg tgtgtccgtg caacgctggc agcttgatct
     6361 ggctgtactg gcaaaatccc gggaagaaga gacgacccgg ccaaccctta aagaactggc
     6421 agcgaagctg tccggaggag tgaatggtta cagtcgctga actgcaggcg ctgcgtcagg
     6481 cgcgccttga tttattaacc ggtaaacggg tggtgtctgt ccagaaagat ggacgaagaa
     6541 ttgaatatac ggcggcctct ctggatgagc ttaaccgggc gatcaatgat gcggagttgg
     6601 tactggggac aacccgccgt cgccgtcgtc cgctgggagt gaggttatga aacgaacgcc
     6661 tgtcctgatt gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acgggggcgg
     6721 cgcaggattt ggcgggcaaa tggcggagtg gttgccacca gcgcagagtg ccgatgcagc
     6781 cctgctgcct gcgttgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg
     6841 gatagcggct aatgcggtgg ccctgcataa ggatcatatt gtcgggcata tgtttctgat
     6901 cagctaccgt ccgaactggc gctggctggg gatgcgggag actgcggcaa aaagttttgt
     6961 cgatgaggtg gaggcggcct ggtcggaata cgccgaaggg atgtttggcg agatcgacgt
     7021 ggaagggaaa cgcacgttta cggaatttat ccgtgaaggt gtgggcgttc atgcgtttaa
     7081 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg
     7141 ttttaaagcc gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccgttt
     7201 tctgcgggcc ggtgtggagg tcgatcgata tggtcgtgcc gttgcgtacc atatctgtga
     7261 ggatgatttt ccgttctctg gtagtggacg atgggaacgg atcccgcgtg aacttcccac
     7321 cgggcgtccg gccatgctgc atattttcga gccggtggag gacgggcaga cccgtggggc
     7381 taatcagttt tacagcgtca tggaacggct gaagatgctc gattccctgc aggcaacaca
     7441 gcttcagtcg gccatagtga aggcgatgta tgcagcgacg attgaaagtg aacttgatac
     7501 cgaaaaggcc tttgaatata tcgccggcgc gccacaggag cagaaggata atccgcttat
     7561 taatattctg gagaagttct ccagctggta tgacacgaat aacgtgacac tgggcggtgt
     7621 caaaattccg caccttttcc ctggtgatga tctgaaacta cagacagcgc aggattcaga
     7681 caatggattt tctgcgcttg aacaggcgct gctgcggtat atcgccgccg gtcttggcgt
     7741 ttcctacgaa cagttgtccc gtgattactc gaaggtcagt tactcaagtg cccgcgcctc
     7801 cgccaatgag tcgtggcgct attttatggg gcggcgaaaa tttattgcgt cccggctggc
     7861 cacgcagatg ttttcctgct ggctggaaga ggcacttctt cgggggatta ttcgtccgcc
     7921 acgggcacgt tttgattttt atcaggcgcg atcagcctgg tcacgggctg agtggattgg
     7981 agccggaaga atggccattg acgggctcaa ggaggttcag gaatcagtga tgcgcattga
     8041 ggccggactg agcacgtatg agaaagagct ggcgctgatg ggcgaggatt atcaggacat
     8101 tttccgccag caggtcaggg aatctgcaga gcgggaaaaa gccggactct cacgtccggt
     8161 gtggatagcg caggcgtatc agcagcagat agcggagagt cgcaggccgg aagaggagac
     8221 aacaccacgt gagacgtaat ctttcacaca ttattgcagc agcattcaat gaaccgctgc
     8281 ttctggagcc cgcctatgcg cgggttttct tttgcgcgct cgggcgcgag atgggggcag
     8341 caagtctttc ggtaccacaa cagcaggtac agcttgatgc acccggaatg ctggctgaaa
     8401 cggacgagta catggccgga ggtaaacgac cggcccgtgt ttaccgggtg gtgaacggta
     8461 ttgcggtact gccggtgacc ggcacgctgg tgcaccggct gggggggatg cggccatttt
     8521 ccggaatgac tggctatgac ggcattgtcg cctgtcttca gcaggcaatg gcagatagcc
     8581 aggtgcgggg catactgctg gacattgaca gtccgggcgg gcaggccgcc ggcgcgtttg
     8641 actgcgctga catgatttac cgcctccggc agcagaagcc ggtctgggca ctgtgtaatg
     8701 acacggcctg ttctgcagcc atgctgctgg cgtcggcctg ctcccgacgg ctggttaccc
     8761 agacatcccg tatcggctcc attggcgtga tgatgagcca tgtcagctat gccggtcatc
     8821 tggcgcaggc cggtgtggat atcacgctga tttatgccgg ggcgcacaag gtggatggca
     8881 atcagtttga agcgttgccg gcagaggttc gccaggacat gcagcagcgg attgatgcgg
     8941 cgcgccggat gtttgccgaa aaagtggcga tgtataccgg tctgtctgtt gatgccgtca
     9001 cgggaacaga ggctgccgtt tttgaaggtc agtccggcat tgaggccggg ctggcggatg
     9061 aattaatcaa tgcgtcggat gccatcagtg tgatggccac ggcgctgaac agtaatgtca
     9121 gaggaggcac tatgccgcaa ttaactgcaa cggaagccgc cgtgcaggag aaccagcgag
     9181 tgatggggat cctgacatgc caggaagcga aaggacgtga acagcttgcc acgatgctgg
     9241 cagggcaaca gggcatgagc gttgaacagg cccgggcgat tctggccgcg gcggcaccgc
     9301 agcagccggt ggcatccgca cagagtgaag ccgatcgcat tatggcgtgt gaagaagcga
     9361 acggtcgtga acaactggca gcaacgctgg cggcgatgcc ggagatgacg gtggaaaaag
     9421 cccgcccgat cctggctgct tcaccgcagg cgaatgccgg gccctcactt cgtgatcaga
     9481 tcatggcact ggatgaggca aaaggggctg aggcgcaggc tgaacagctg gctgcctgcc
     9541 cgggaatgac tgtggagagc gcccgggctg tgctggctgc gggatcaggt aaggcagaac
     9601 cggtctctgc atccacaacc gccctgtttg aacatttcat ggcgaaccat tcaccggctg
     9661 cggtccaggg gggcgtgtca caggcgtcag aagacggtga tgcggacgtg aaaatgctca
     9721 tggccatgcc atgaagtcag tgctgaacat caatacgagg ttttaacaat atggtgacga
     9781 aaaccatcac tgaacagcgt gcggaagtac gtatttttgc cggtaatgat ccggctcaca
     9841 ccgccacagg cagcagcggg atttcctcgg caacaccggc actgacaccc ctgatgctgg
     9901 atggggccac cgggaaactg gtggtctggg acggacagaa agccggtagt gcggttggca
     9961 tactggtact gccgcttgaa ggcacagagg cggtgctgac gtattacaag tcggggacct
    10021 ttgcgacgga ggcaatccgc tggcctgaaa gtgtggatga acacaaaaag gccaacgcct
    10081 ttaccggcag tgccctgagt cacgcggcgc tgccgtaaca cgttatcagg ccaccgcgtt
    10141 ggcctgactg atttcttaat gaaaggaact gatttatggg attgtttacg acccgccagt
    10201 tactcggtta taccgaacaa aaagttaaat tccgtgcgct gtttctggag ctgtttttcc
    10261 gccgcacggt gaatttccac accgaagagg tgatgctgga caaaattacc ggaaaaacgc
    10321 cggtggcggc ctatgtctcc ccgatcgttg aaggaaaagt gcttcgccat cgcggtggtg
    10381 aaacccgcgt gttacgtccg ggctacgtca agcccaaaca cgaatttaat taccagcagg
    10441 cggttgagct ccttcctggt gaagatccgg ctcagctgaa cgacccggcc taccgtcgtc
    10501 tgcgtatcat taccgataac ctcaaacagg aagagcacgc cattgtccag gtggaagaaa
    10561 tgcaggcggt gaatgccgtg ctgtatggca aatacaccat ggaaggggat cagtttgata
    10621 ctgtcgaggt ggatttcggg cgctctgaag gaaataacat tgagcaggct gacggtaaaa
    10681 aatggtctga gcaggaccgt gatacgtttg atccgacgca tgatattgac ctctactgcg
    10741 atcaggccag cggccttgtg aatatcgcca ttatggacgg tacggtctgg cgtctgctga
    10801 atggctttaa gctgttccgc gaaaaactgg atacccgtcg cggctcaaat tcacaactcg
    10861 aaacggcagt gaaagacctg ggggcggtgg tgtccttcaa ggggtattac ggcgatctgg
    10921 ccattgtggt ggcgaaaacg tcttatgtgg cagaggacgg taccgaaaaa cgttatctgc
    10981 cggagggcac actggtcctg gggaatacgg cagcagaggg cattcgttgc tatggtgcca
    11041 ttcaggatgc gcaggcgttg tccgaaggtg tggtggcctc ttcccgttat ccgaaacact
    11101 ggctgactgt gggcgatccg gcccgtgaat tcaccatgac gcagtccgca ccgctgatgg
    11161 tgctgccgga tccggatgag tttgtggtgg tacaggtgaa ataatccgtg agcgggggcg
    11221 aaatgccccc gtgtcttttt tcacaggagg ctgagatggc aacaaaagaa gaaaatctga
    11281 atcgtcttcg tcaactggct ggcctgctgg ggcgcgaggc ggatatgtcg gggagtgctg
    11341 cggatattgc tcaacgtgtg tctgagtggg aagaggagct tgctgtttcc ccggagggca
    11401 ttatgcactc tgatgagagc ggggctgatc aaaatcacac agacgatggt gagcagttgc
    11461 acaacactga tgctacggat gatgttaaag cggtccgtgt gcggaaatgc ctgcatgtga
    11521 tggggtattg cccggagaca ggccgtcccg ttgaactgac gtaccggggc atgcgtgtta
    11581 tggtgccatc accactggcg acagccatga tacagcacgg aacggctgag catgcgtgat
    11641 tttcagaatg cctttgatgc tgcccttgcc ggggtggaca gcacgattgt tgaagtgatg
    11701 gggctctgtg cgcagttcac ctcgggagca cagcgtggaa gcgaagttca gggggttttt
    11761 gacgatccgg agtcgctggg ttttgccggt ggcggggtcc gtattgaagg aagcagcccg
    11821 tcattatttg tgcggacgga tacggtgcgt gccgtgcggc gtggtgacac gctgaccatt
    11881 aacggcgaga tgttctgggt ggatcgtgtt tctccggatg acgggggcag ctgttatctc
    11941 tggctcaacc gtgggcaacc acccgctgtt aaccggcgac gataaacgca gggtgaaatt
    12001 atggcgataa aagggcttga tcaggcgatt gataatctga gccgggttcg taaaaacgcc
    12061 attccggcgg cttcagcaat gacgattaac cgcgtggcca caacggcgat taatcagtct
    12121 tcatcacagg ttgcccggga gacaaaggta cgccggaaac tggtaaagga acggtccaga
    12181 ctgaaacggg cgacggtcag aaatccgaac gcaaaaatta tcgttaaccg cggtgatctt
    12241 ccagtgatta agctggggat caggatgctg ggccgtcgtc cgaacagcat acttaaagcc
    12301 ggtcagcatc ggtatcagcg ggcattcatt cagcgattaa aaaacggtcg ctggcatgtc
    12361 atgcagcgtg tggccgggaa aaaccgttac cctattgatg tggtgaaaat cccgatggcg
    12421 gccccactga aacaggcgtt tgatgagaat gttgaccgta tccggtgtga acgcctgccc
    12481 ggagaactgg catccgcgct gaaacaacaa ctgaggattg cgataaaacg atgaaacaca
    12541 ctgacattcg tgccgcagtg ctggatgcac tcgagcagca tgaacacggg gcgacgctgt
    12601 ttgatggtcg ccccgttgtt tttgacgaag aggattttcc tgcgatcgcg gtttatctga
    12661 cggatgcaga gtataccggt gaagagctgg atgcagatac ctggcgggcc acgctgcata
    12721 ttgaggtgtt tttaccggca caggtaccgg attcagagct tgatcagtgg atggaaagcc
    12781 ggatttaccc ggcgatgacc gcgatcccgg cactggcagg actgattacc acgatggtta
    12841 cgcagggcta tgagtatcgt cgtgatgacg atatggcgtt atggagttct gcagatctga
    12901 cttattccat tacatacgag atgtgaggac gatatggcaa caccaaatcc ccttgagccg
    12961 gtaaaaggtg ccggtaccac tctgtgggtt tacaacggca aggctgatgc ttatgcaaac
    13021 ccgttgtcag acgatggctg gcagcgactg gctaaggtga aggatctgac gccgggcgag
    13081 atgacggctg aaccctacga tgataactac ctggatgatg aagacgcgga ctggaccgcg
    13141 accgggcagg ggcagaaatc tgcaggtgat accagtttta cgctggcctg gaaaccggga
    13201 gaggaaggtc agaaagggct tataggctgg tttgaaagcg gcgatgtccg ggcctataaa
    13261 atccgttttc cgaatggcac ggtggatgtg tttcgtggct gggtcagcag tatcggtaag
    13321 gccgtgacgg cgaaagaagt gatcacccgc acggtgaaag tcactaacgt gggtaaacct
    13381 tctgtagcgg aagaacgcag caaaattacg ccggtcactg cgattaaggt aacgccgaca
    13441 ggtacggttg aaaaagggaa aacaaccacc ctgaccgtta ctgtggaacc ggaaaatgca
    13501 acggataaga cattcagggc gatttccgcc gatccatcaa aagccaccat tagcgtgaaa
    13561 gatatgacga ttactgtgac gggggttaag gatggaaaag tcagcatccc tgtgatttcc
    13621 ggtaatggtc agtttgctgc ggtggctgaa attaccgtta ataatgtgcc gggtggctaa
    13681 agagctgaga gataagcgat gttcctgaaa acagaacaat ttgaatataa cggtgtatcc
    13741 gtcacgcttt ctgagctgtc tgcgctgcag cgtattgagc atcttgccct cctgaaacgg
    13801 cgggcagaag aggctgaagc cagcggcaac ctgcaggtga gtgtggaaga tcttgtcaga
    13861 accggcgcgt ttctggtggc gatgtccctg tggcataacc atccacagaa aacgcagtca
    13921 ccgtcaatga atgaggccgt gatgaagata gagcaggaag tgctcaccac ctggcctgcc
    13981 gatgccattg cccgggcgga agacgtggtg ttgtgcctgt ccgggatgat cgaagctgtt
    14041 cgtccggata ctgatattac tgaagtggcg aaaaataaca cgctgactga tgatgatttt
    14101 tctgcgggaa agtcttcgac ggcgagctga actttgccct cagactggcg cgtgagatgg
    14161 ggagacccga ctggcgcgcc atgcttgccg ggatgacatc caccgaatat gccgactggc
    14221 accgttttta ccgcacgcat tattttcagg atacccagct ggatatgcat ttttccgggc
    14281 tgacgtacgc tgtactcagc ctgttttttt gcgatccgga tatgcatccc tctgatttca
    14341 gtctgcttgt cccccggcat gaggaagaac aggtggagag gccggatgag gacaaaatgc
    14401 tgatgcagaa agcggcagga cttgccggag gcgtccggtt cggtggggac ggagggcgcg
    14461 atattttatc gtctgcggat gtggcggatg tcatggtgga tgatgccgca ttaatgatgg
    14521 cttcagcggg gattccggga ggtgtgagat atgtcccagc cggttggtga tcttgttatt
    14581 gaccttagtc tggatgctgt ccgtttcgat gagcagatga gccgggtaag gcgtcatttt
    14641 tcaggtctgg ataccgacgc cagaaaaacc gccagtgctg ttgaacaggg cctgagccgc
    14701 caggcgctgg ctgcacaaaa agcagggatt tccgtcgggc agtataaagc ggccatgcga
    14761 accctgcccg cacagtttac ggatatcgcc acgcagcttg ccggtggtca gaatccctgg
    14821 ctgatcctgc tgcaacaggg cggtcaggtg aaggactcct tcggcgggat gatccccatg
    14881 ttcagggggc ttgccggtgc gatcaccctg ccgatggtcg gggtcacctc gctggcggtg
    14941 gcgacaggtg cgctggtgta cgcctggtac cagggagatt ccacgctttc agcgtttaat
    15001 aaaaccctgg ttctttccgg taatcagtcc ggactgactg ccgatcgcat gctgacgctc
    15061 tccagagccg gacaggccgc agggctgacg tttaaccagg cgagtgagtc actggcagcc
    15121 atggtgaatg ccggtgtgcg tggtggtgaa cagtttgatg ccatcaacca gagtgtcgcg
    15181 cgttttgctt ctgcatccgg tgtggaggtg gacaaggttg cagaggcttt cggaaaactg
    15241 accactgacc cgacgtcggg gctgattgcg atggcgaagc agttccataa cgtgacggcg
    15301 gagcagattg cgtatgttgc tcagttgcag cgttccggcg atgaaaccgg ggcattgcag
    15361 gctgcgaacg aggccgcaac gaaagggttt gatgaccaga cccgccgcct gaaagagaac
    15421 atgggcacgc tggagacctg ggcagacagg acagcgcggg cattcaaatc catgtgggat
    15481 gcggtgctgg atattggtcg tcctgatacc gcgcaggaga tgctgattaa ggcagaggct
    15541 gcgtttaaga aagcagacga catctggaat ctgcgcaagg atgattattt tgttaacgat
    15601 gaagcgcggg cgcgttactg ggatgatcgt gaaaaggccc gtcttgcgct tgaagccgcc
    15661 cgaaagaagg ctgagcagca gactcaacag gacaaaaatg cgcagcagca gagcgatacc
    15721 gaagcgtcac ggctgaaata taccgaagag gcgcaga
//
LOCUS       DAOTRK010000111        15507 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.111, whole genome shotgun sequence.
ACCESSION   DAOTRK010000111 DAOTRK010000000
VERSION     DAOTRK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.111"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..680
                     /locus_tag="QIE41_003372"
     CDS             <1..680
                     /locus_tag="QIE41_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3532224.1"
                     /translation="IIFGLINAILIYYLNVVSIIITISMQSLLFGMLMWLTNGHSIYD
                     LPDWWITQRSFLSFEFGGEDYQIGLPLVVMLGMAFLTWVIMNKTHIGRQLYAVGGNTE
                     SARRIGIRVSLIYLFAYGYLGAAAAIGGMLQAYRMSEVVPNALVGGELDVLAAAVLGG
                     ASLSGGRGTVIGTLMGVFLIGILKNGLNLIGVSSYFVNIVIGVVIVSAICVTHYKKRK
                     ETDVGFV"
     gene            694..1740
                     /locus_tag="QIE41_003373"
     CDS             694..1740
                     /locus_tag="QIE41_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3532225.1"
                     /translation="MNKRIFFPLDGTRSGLLSICFIVVVLFSLAMPGRFFTDNTFLSI
                     AFQLPELGLLTFAMFIPMLSGGLNLAIIGTANLTSLFMAWVLIQYVPADASTGSQLFW
                     LFLAIVGAMLIAIIIGAITGLIISHIGAHPILVTLGSMTIISGIGIYLTKGAALSGMP
                     PVVRSIGSEVIMGIPVPMMIFIVTTVALALFLGKTRTGKTIYMCGSNINATWFSGIRT
                     DRVLIAIYAISSMLCVLAGLIMLARFNSARMGYGDSYLLLTVLAIVLGGTDPFGGAGK
                     VSHVFCALLVLQVIATGLSLLGLSLHFNLAVWGITLIAALAFKFIKQKWQLQRDMNKS
                     RKIFQQQLITREGK"
     gene            1740..2603
                     /locus_tag="QIE41_003374"
     CDS             1740..2603
                     /locus_tag="QIE41_003374"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDS3532226.1"
                     /translation="MRRHPLGIYEKALPRNTSWVEKLALAKSCGFDFVEMSVDENDER
                     LARLDWSLTERMELISAIQQTGIRIPTMCLSAHRRFPFGSHDRDTRQQARTIMLKALR
                     LAQDLGIRTIQLAGYDVYYEPQNEETIAWFEDAMAWTAEQAAAAQVMCAVEIMDTPFI
                     NSISKWKVLDTKINSPWFTVYPDVGNLTAWGNELESELVKGIDRIAAIHLKDTYPVTP
                     DSPGQFRDVPFGEGCVDFIKLFKILRNLNYRGTFLIEMWTEKATEPLLEIIHARQWIE
                     EKMRLAGWDNI"
     gene            2620..4098
                     /locus_tag="QIE41_003375"
     CDS             2620..4098
                     /locus_tag="QIE41_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDS3532227.1"
                     /translation="MERYFIGVDLGGTETKAGIYTAEGREIGVSSQVLPLLSPCPGFC
                     ERDMEALWKVTCQVISLSMSENNITASQIDGISFSAHGKGLYLLDRQGKPLRHGIVSS
                     DSRAQQLVASWKEQGIDRESYHRSLQQLWPSHPVALLHWLKKHETDNYGRIGTILMVH
                     DYIRSRLTGKLACEETNISGSNFYNQYTSAYDAELFRLFEIKEVAGKVPDIIGSADLA
                     GVVTSEAASQCGLLAGTPVFGGVFDVVGAALTSGIYDSTTLSAVAGTWSIATRIFDRI
                     IPSDYPYVWGKFSIPGTFFAHEGSPTSASNLTWFLQQFLPDLPDSYRVINEWVERGYE
                     KQNDILFFPWLFGSNYHEALSGGFLGLCGHHKREDLVYAIYQGIVFSHLLHQDRLIEI
                     DKSLQTIRFSGGPTHSRIWMQMFCDASNMPLEIVDIEQSGCRAAAMCAAVGGGAYSGF
                     KQAMKATHLNVIRLEPNVEKAHMLRRRFARFKQVASALSSVV"
     gene            4587..5801
                     /locus_tag="QIE41_003376"
     CDS             4587..5801
                     /locus_tag="QIE41_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HDS3532228.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDANRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            5868..7151
                     /locus_tag="QIE41_003377"
     CDS             5868..7151
                     /locus_tag="QIE41_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532229.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRSELGLSATEFGFLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRAMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            7404..8321
                     /gene="oxyR"
                     /locus_tag="QIE41_003378"
     CDS             7404..8321
                     /gene="oxyR"
                     /locus_tag="QIE41_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDS3532230.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPSERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8304..9704)
                     /gene="sthA"
                     /locus_tag="QIE41_003379"
     CDS             complement(8304..9704)
                     /gene="sthA"
                     /locus_tag="QIE41_003379"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDS3532231.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            10036..10686
                     /gene="fabR"
                     /locus_tag="QIE41_003380"
     CDS             10036..10686
                     /gene="fabR"
                     /locus_tag="QIE41_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDS3532232.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            10686..11045
                     /locus_tag="QIE41_003381"
     CDS             10686..11045
                     /locus_tag="QIE41_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDS3532233.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(11085..12185)
                     /gene="trmA"
                     /locus_tag="QIE41_003382"
     CDS             complement(11085..12185)
                     /gene="trmA"
                     /locus_tag="QIE41_003382"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDS3532234.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      12298..12488
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12553..14397
                     /gene="btuB"
                     /locus_tag="QIE41_003383"
     CDS             12553..14397
                     /gene="btuB"
                     /locus_tag="QIE41_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDS3532235.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGEMIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVTVGHGNIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            14342..15199
                     /gene="murI"
                     /locus_tag="QIE41_003384"
     CDS             14342..15199
                     /gene="murI"
                     /locus_tag="QIE41_003384"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDS3532236.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIITAVQKRYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         3648 a         3463 c         4093 g         4303 t
ORIGIN      
        1 gcatcatttt tggtttgata aacgctattc ttatttatta ccttaatgtc gtttccatca
       61 tcattacgat tagtatgcag tcgttactgt tcggtatgtt gatgtggttg actaatggtc
      121 acagtattta cgatttaccg gattggtgga ttacgcagcg ttctttcctg agttttgaat
      181 ttggcggaga agattaccag atcggattac cgttagtggt tatgctggga atggcatttc
      241 tgacgtgggt gataatgaac aaaacccata ttgggcgtca gctttatgcc gtgggaggga
      301 ataccgaatc agcaagacgt attgggattc gggtgtcgct aatttatctg tttgcttacg
      361 gttatcttgg tgccgctgca gcaattggtg gaatgctgca agcttaccgt atgagtgaag
      421 ttgtgccgaa tgcattggtt ggcggagagt tggatgtgct ggctgcagcg gtattgggcg
      481 gtgccagttt atcaggcgga cgtggtacgg tcattggtac gcttatgggc gtatttctta
      541 tcggtattct taaaaacggc cttaatttga ttggtgtctc gagttatttc gtcaatatcg
      601 ttattggggt ggttattgtc tcggcgattt gtgtgaccca ttacaaaaag cgtaaagaaa
      661 ctgatgttgg tttcgtctaa cgggaggatc gaaatgaaca agcggatatt ttttccacta
      721 gatggtaccc gttcaggctt attatcaata tgttttattg tggtggtgtt atttagtctg
      781 gcaatgccgg ggcgtttttt caccgacaat acttttctga gcattgcatt tcaattgcca
      841 gagcttgggt tgctcacgtt tgcgatgttt ataccgatgt tgagcggggg gcttaatctg
      901 gcaatcatcg gtactgcgaa tctgaccagc ctgtttatgg cctgggtatt gattcaatat
      961 gtacccgcag atgcatctac aggtagtcag ttgttttggc tcttcctggc tattgtcggg
     1021 gcgatgctta ttgccatcat cattggagct attactgggt taattatttc tcatataggc
     1081 gctcacccta ttttggtcac attgggtagc atgaccatta ttagtggtat cggtatttac
     1141 ctgactaagg gggcagcttt aagtggtatg ccaccagtag tccgcagtat tgggtctgaa
     1201 gttatcatgg ggatcccagt accgatgatg atttttatcg tcaccaccgt tgctttagct
     1261 ctgttcctcg gcaaaacccg tacgggaaaa acgatttata tgtgtggcag taatattaac
     1321 gccacatggt ttagcggtat tcgcaccgac cgcgtattaa ttgctatata tgctatatcc
     1381 agcatgttgt gtgtattagc aggtcttatt atgctcgcgc gatttaattc ggcgcgtatg
     1441 ggctatggcg attcctattt gctcttgacg gttcttgcaa ttgtattggg aggcacagat
     1501 ccattcggtg gggcgggtaa agtcagtcat gttttttgtg ccttacttgt gcttcaggtt
     1561 attgctaccg gccttagtct gttgggactt agtttgcatt tcaatctggc ggtatggggg
     1621 atcacattga tcgccgcatt ggcatttaaa ttcatcaaac aaaaatggca gttgcagcgt
     1681 gacatgaata agagccggaa aatctttcaa caacaattga tcactcgtga gggaaaataa
     1741 tgcgacgcca cccgttaggt atttatgaaa aagcgctacc caggaatacc agttgggtag
     1801 agaaactagc cctggctaaa tcttgcggtt ttgattttgt tgagatgtcg gtagatgaga
     1861 atgatgaacg actcgcccgc ctggactggt cgctcaccga gcgtatggag cttattagcg
     1921 ccattcagca aacggggatc cgtattccga caatgtgcct gtctgctcac cggcgttttc
     1981 catttggtag ccatgataga gatactcgtc agcaggcgcg tacgattatg ttgaaggcgt
     2041 tacgcctggc gcaggatttg gggattcgaa caatccaact ggcaggctat gacgtttatt
     2101 atgagccgca gaatgaagaa actatcgcct ggtttgaaga tgcgatggcc tggacagctg
     2161 aacaggccgc agcggcacaa gttatgtgtg ctgtagaaat catggatacg ccatttatta
     2221 attctattag taaatggaaa gtcctggata caaaaattaa ttccccttgg tttaccgttt
     2281 atccggatgt tggcaatctg accgcatggg gtaatgaact ggaaagtgag ctggtaaagg
     2341 ggattgatcg aattgccgca atccatctta aagatacgta cccagtaacg cctgattctc
     2401 cagggcaatt tcgtgacgtt ccttttggtg aagggtgtgt agattttatt aaattattta
     2461 agattctgcg taatcttaat tatcgcggaa cgtttctgat agaaatgtgg acagaaaaag
     2521 cgacggaacc tctattagaa attattcatg cacgtcaatg gattgaagag aaaatgcgcc
     2581 ttgccggctg ggataatatt tagaaactaa ggaggggtca tggagcggta ttttattggt
     2641 gtcgatttag gtggtacaga aaccaaagcg ggaatttata cggcagaagg acgtgaaata
     2701 ggtgtctctt cgcaagtgtt acctctgtta agcccatgtc ctggtttttg cgaacgcgac
     2761 atggaggcgt tatggaaggt aacgtgccag gtcatttcac tgtcgatgtc agagaacaat
     2821 attactgcga gtcagattga tggcatcagt ttttctgctc atggtaaagg tttgtacctg
     2881 ctcgaccggc aaggtaagcc cttacgacac ggcattgttt cctctgattc tcgcgcacaa
     2941 caactggttg caagctggaa agaacaagga attgaccgcg aaagctatca ccgtagtctg
     3001 caacagctct ggccatcgca ccctgtagca ctattgcatt ggttgaaaaa acatgagact
     3061 gacaattatg gccgcatcgg aaccattttg atggttcatg attacatacg ttcacggctt
     3121 actggcaaac tggcttgtga agaaaccaat atttcaggca gtaattttta taatcaatat
     3181 accagtgctt atgatgctga actttttcgc ctatttgaga ttaaggaagt tgcgggtaag
     3241 gttcccgata ttattggctc cgctgattta gcaggggttg tgacatctga agcagccagc
     3301 caatgtgggc ttctcgccgg aacgcctgtt tttggtggtg tttttgatgt cgttggtgcg
     3361 gcgctcactt ctggcatcta tgacagtaca acgttaagtg cggttgctgg cacctggtca
     3421 attgcaacgc gaatctttga tcgcattatt ccttctgatt atccctatgt atgggggaaa
     3481 tttagcattc caggtacatt tttcgcccat gagggaagcc ctacatcagc cagtaatttg
     3541 acatggttct tgcagcaatt tttaccggat cttcctgaca gctatcgggt gattaatgag
     3601 tgggtggaac gagggtatga gaagcagaat gatattctct ttttcccgtg gttgtttggc
     3661 tcgaattacc atgaggcatt aagcggggga tttttggggc tgtgtgggca tcataaaaga
     3721 gaagatttgg tgtacgcgat ttatcagggc attgtctttt ctcatttgtt gcatcaggac
     3781 cgtcttattg agattgataa gtcattgcaa acgattcgtt tctctggcgg gccaacgcat
     3841 tccagaatct ggatgcagat gttttgtgat gccagcaaca tgcctcttga aattgtcgat
     3901 attgaacagt caggttgtag agcggctgca atgtgtgcgg cagtaggagg aggggcttat
     3961 tctggattca aacaggccat gaaggccacg catctcaacg tgattcgtct tgaaccgaat
     4021 gtagaaaaag cacacatgct caggcgacgg tttgctcgct ttaaacaggt tgccagtgcg
     4081 ctgagttccg ttgtttgatg gaaaacaaag cggattctgg agagccacaa agttcacatt
     4141 ggccttcgtt attttgagtt gagaaatgcc tgaaatagac tgtgacttca agggttaatg
     4201 ggttgcggaa gggaggaacg gctaccctta ccgcgaccaa tattaatcgt atttcttcct
     4261 gtttgtgtat cttcttttca tctctcattc cgaaaaatct ttttaaaata aacatatcga
     4321 tacgtattga attaagtaaa atatgattat catttgttat tgttatgact ctcttcctta
     4381 gaggaatttg ttatgtgatt atttaacgtg ttgttttaac aatagtaatt aatagtgtgg
     4441 atgaaatttg aggatattct tgttcctcgg cggaaatttt tatttttaat gtgatcacgg
     4501 ttgaataatc gccctttctc tcttgtatgg tagtaggcgt aaaattaagt ttgaagcgtc
     4561 tatttacaac acaggagatt gcaataatga aaattattgc tgcagatgtt tttgttactt
     4621 gcccagggcg taatttcgtt acgcttaaaa taaccactga aagcggactt tgtggattgg
     4681 gagatgccac attaaatggt cgcgaattgt cggtcgcttc ttatctgaaa gatcatcttt
     4741 gtccacaatt aattggccgc gatgccaacc gtatcgaaga tatctggcaa ttcttttata
     4801 aaggtgctta ctggcgacgt ggccccgtca cgatgtcagc catttctgcc attgatatgg
     4861 cgttatggga tatcaaagct aaagcggcaa atatgccatt gtatcaactg cttggtggtg
     4921 cttctcgtga aggcgtgatg gtttattgcc acaccaccgg gcgtacgatt gatgaagtcc
     4981 ttgaagacta tgcaaagcac cagcagatgg gctttaaggc gattcgtgtg cagtgcggtg
     5041 taccgggtat gcaaaccact tatggcctgg cgaagggcaa aggactggcg tatgaaccgg
     5101 caaccaaagg cctgtggcca gaagagcagt tgtggtcttc agaaaagtat ctcgatttta
     5161 cgccgaagtt attcgaagcg gtgcgtaata agttcggttt taacgaacat ctgttgcatg
     5221 acatgcacca tcgcctgacc cccatcgaag cggcgcgctt tgggaagagc attgaagatt
     5281 accgcctgtt ctggatggaa gatccgacgc ctgccgagaa tcaggaatgt tttcgtctga
     5341 ttcgtcagca taccgtgacg ccgattgcgg tcggtgaagt gtttaacagt atctgggact
     5401 gcaagcagtt aatcgaagag cagctgattg actatattcg cacgacgatt acccatgcag
     5461 gcggcattac cgggatgcga cggattgctg attttgcatc gctttatcag gtacgtaccg
     5521 gttcgcatgg cccttcagat ttatcgcctg tttgccatgc ggcggcgctg cattttgacc
     5581 tctgggtgcc aaactttggc gttcaggagt acatgggcta ttccgaacaa atgcttgaag
     5641 tcttcccgca tagctggcgc ttcgatgaag gttatatgca cccaggcgat gaaccggggc
     5701 tgggtatttc tttcgatgag aaactggcgg cgaaatatcc gtatgacccg gcgtatcttc
     5761 ccgtcgcacg actggaagat ggcacattgt ggaactggta ataacggttc gcttaattat
     5821 tttctgaata gtcatcaaat cgattaattc cgctcaagga gctaaaaatg gacgttgatg
     5881 tgtcaaccag tgttgcgggt aataaaccgc aacggattcg tcgtattcag acagtgaccc
     5941 tggttttatt atttatggcg gggatcgtta attttctcga ccgttcgtca ttgagcgtgg
     6001 caggcgaagc gattcgtagc gagttaggat tatcggcgac agagtttggt tttttgcttt
     6061 ctgcattttc tctgtcttat ggtttttcac aacttccttc cggtattttg ctagaccgct
     6121 ttggcccacg aatagtttta ggcgcaggct taatattctg gtcattaatg caggcattaa
     6181 caggaatggt taattctttt agccacttta ttttaatgcg tatcggtctg ggtattggcg
     6241 aagcgccatt tatgcctgcg ggagtaaagt cgatcaccga ctggtatgca caaaaagaac
     6301 gcggcacggc gctggggatt tttaactcat ctaccgttat cggtcaggct atcgcgcctc
     6361 ctgctcttgt gttgatgcaa ctggcatggg gctggcgggc aatgttcgtc atcatcggcg
     6421 tggcggggat tctggttggg atctgttggt acgcgtggta tcgcaaccgg gcgcagtttg
     6481 tcctgactga cgaagaacgg acgtatctct ccgcgccggt taaaccgcgt ccacagctgc
     6541 aatttagcga gtggctggcg ctgtttaagc atcggacaac ctgggggatg attttaggtt
     6601 tctctggcgt caattatacc ggttggctct acatcgcgtg gttacccggt tatttgcagg
     6661 ccgagcaagg tttcagtctg gcgaaaaccg gttgggtggc ggcgattcct ttcctcgcgg
     6721 cggcagtcgg gatgtgggta aacggtattg ttgtcgatcg actggcgaaa aaaggctacg
     6781 acctggctaa gacgcgtaaa acggctattg tctgcggttt gatgatgtcg gcattaggca
     6841 cgttgctggt cgtgcaatct tcctcgccag ctcaggctgt agcgtttatc tcaatggcgc
     6901 tattctgcgt gcatttcgcg ggaacgtctg catggggact ggtgcaggtg atggtgtcag
     6961 aaacaaaagt ggcttccatc gccgggattc aaaactttgg cagttttgtt tttgcttcct
     7021 ttgctccgat cgtaaccggt tgggtagtgg ataccacaca ctcgtttaat ctggcgttgg
     7081 ttatcgcggc ctgtgtgacg ttcaccgggg cgttgtgtta cttctttatt gtcaaagatc
     7141 gcattgagta accgttacta tcaggctctc ttgttgtggg cctgtagaat aaaaaaaaag
     7201 cggatcctgg agatccgcaa aagttcacgt tggctttagt tattcgagtt gagaaactct
     7261 cgaaacgggc agtgacttca agggttaaaa gaggtgccgc tccgtttctg tgagtaatta
     7321 tcagtcagaa tgcttgatag ggataatcgt tcgttgctat gctacctatc gccgcgaact
     7381 atcgtggcaa tggaggatgg ataatgaata ttcgtgatct tgagtacctg gtggcattgg
     7441 ccgaacaccg ccattttcgg cgtgcggcag attcctgcca cgttagccag ccgacgctta
     7501 gcgggcaaat tcgtaagctg gaagatgagt tgggcgtgat gttgctggag cggaccagcc
     7561 gtaaagtgtt gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt accgtactgc
     7621 gtgaggtgaa agtccttaaa gagatggcaa gccagcaggg cgagacgatg tccggaccgc
     7681 tgcatattgg tttgattccc acggttggac cgtacctgct accgcatatt atcccgatgc
     7741 tgcaccagac ctttccaaag ctggaaatgt atctgcatga agcacagacc caccagttac
     7801 tggcgcaact ggacagcggc aagctcgatt gcgtgatcct cgcgctggtg aaagagagcg
     7861 aagcattcat tgaagtgcca ttgtttgatg aaccaatgct gctggctatc tatgaagatc
     7921 acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct ggcaggggaa aaactgctga
     7981 tgctggaaga cggtcactgt ttgcgcgatc aggcaatggg tttctgcttt gaagccgggg
     8041 cggatgaaga tacacacttc cgcgcgacca gcctggaaac actgcgcaac atggtagcgg
     8101 caggtagcgg gatcacttta ttgcctgcgc tggctgtgcc gtcggagcgc aaacgcgatg
     8161 gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc ctggtttatc
     8221 gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc cgcgcaagaa
     8281 tggatggcca tttcgataaa gtgttaaaac aggcggttta aaccgtttaa cgcagctacc
     8341 cgataggctt ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg
     8401 ttgccgccac ctttctgttc cataatcgcc tgaccgatat gaataatttc ggcagcgcgc
     8461 tcgccaaagc agtgaatgcc cagaatctct ttcgtttccc gatggaacaa aattttcagc
     8521 gtgcccacgt tcatgccgac gatttgtgca cgtgccagat gtttaaactg ggcgcggccc
     8581 acttcatatg gcactttcat cgcggtcagc tgctgttcgg ttttgcccac agagctgatt
     8641 tccgggatgg tgtaaatgcc ggtagggata tcttcaatca gatgtgcggt ggcttcacct
     8701 ttcaccagcg cctgcgcggc aatgcgcccc tggtcatagg ccgccgacgc caggctcgga
     8761 tagccaatca cgtcgcccac cgcgtaaacg tgcggctgcg cggtctgata catgctgttg
     8821 accttcagct gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc cagcgaatcg
     8881 gtattacccg tgcgaccgtt ggcatagagc agacagtcag ctttcagttt ttttcccgac
     8941 ttcagatgca tgatcacacc gtcgtcacag ccttcgatct tctcgtactc ttcgttgtga
     9001 cgaatcacta cgccactgtt ccagaagtga taggagagag aatctgacat ctcttgatcg
     9061 agaaatgcca gcaggcgatc gcgggtgttg atcagatcca cttttacatc cataccgcgg
     9121 aagatcgacg catattcaca gccgatcact ccagcaccat agataagtac atggcgcggt
     9181 tcgtggtgca tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca
     9241 tctgttggat gatatggacg agagccgcaa gcaataacaa atttttcagc ggttagtgtc
     9301 tcgacgctgc cgtccgggca atccagcgcc agcgtgtgct cgtcaacaaa gcgagcgttt
     9361 ccctgcaata tttcacagtg attacgttcg taaaatccct gacgcatgcg cgtttgttga
     9421 ttaatcacgt tatcggcatg gttaaggata tcggcaaaag aagagcggag cagtcgcgaa
     9481 tggtcgctgt aaagtgggtt ttgattgaat tctataatgc ggctgacggc gtgacggaga
     9541 gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc caacattttg ataacgctcg
     9601 ataactgcga cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc gccgccgggg
     9661 ccggaaccta tcactatggc atcgtaatcg taggaatgtg gcatggtagg gcttacctgt
     9721 tcttatacat aaaagcaaca gaatggtaac attttatcgc gggtaagcca attgatcccc
     9781 gtcatttatc tggctatatc gtgagcggcc tttgctttgt ctgtttctta cttttgacct
     9841 gaacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg tttccgatgt
     9901 acgtaaaaga accggccaaa gaattgcagt aaatatgttt tattgcgtta ccgttcattc
     9961 acaacactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
    10021 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
    10081 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
    10141 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
    10201 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
    10261 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgcacctc ggtctccaca
    10321 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
    10381 ggcacctccg ctgcgtttcg tgctgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
    10441 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
    10501 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
    10561 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
    10621 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
    10681 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgtta gttgctggct
    10741 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
    10801 cgattatttc cctggtgctg acggtttatt gcctgcatca acgttatctt aatcgcacca
    10861 tgccggtagg cttgccgggt ctggctgctg cctgttttat tcttggcgta ctgctgtaca
    10921 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactgt
    10981 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
    11041 agtaatcggt atcatgccgg atgctggcgc acccggcatg gattttactt cgcggtcagt
    11101 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttca
    11161 accttgtgcg tctggcttaa tgtttccaga ttcttgcata acgtttccgg attgcaggag
    11221 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcaccgtc cagaccgctg
    11281 cgtggtgggt cgacaaaaat ggtttcgcac tgataactct ttaaatcgat cccttgcagg
    11341 cggttaaact cgcgtacgcc attcatcgcc tgagtaaatt cttctgccgc catacgaata
    11401 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gaccgacggc
    11461 ttagcgattt cggtggctaa tacccgatca aaattgcgcg ccagtgctaa tgaaaagtta
    11521 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggtgac gtccagcgcc
    11581 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
    11641 cggtagatca tctctttccc tgcgactggc agacgttcat cgatgtaatc ctggtccagc
    11701 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgctcgcagt
    11761 gcatcgcgca gggcttccgc ttcctgacgc cactcatcat ccagcttctt atggtacagc
    11821 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
    11881 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgataagt
    11941 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
    12001 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcacgcat ccggtaatga
    12061 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
    12121 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
    12181 gtcatgatgt atcctgaaaa attaaagtac gcgcgattgt agggattgct catcagatgt
    12241 ccagatcttg atgtattcct atttgtgagc tacgtctgga cagtaacttg ttacaacctg
    12301 tagcatccac ttgccggtcc tgtgagttaa tagggaatcc agtgcgaatc tggagctgac
    12361 gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg acactgccat ccggtgggaa
    12421 gtcatcatct cttagtatct tagatacccc tccaagcccg aagacctgcc ggccaacgtc
    12481 gcatctggtt ctcatcatcg cgtaatattg atgaaacctg cggcatcctt cttctattgt
    12541 ggatgcttta caatgattaa aaaagcttcg ctgctgacgg cgtgttccgt cacagccttt
    12601 tctgcttggg cacaggatac cagcccggat actctcgtcg ttactgctaa ccgttttgaa
    12661 cagccgcgca gcactgtgct tgcaccaacc accgttgtga cccgtcagga tatcgaccgc
    12721 tggcagtcga cctcggtcaa tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa
    12781 aacggcggtt caggtcagct ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg
    12841 ttggtgttaa ttgatggcgt acgcctgaat ctggcagggg tgagtggttc tgccgacctt
    12901 agccagttcc ctattgcgct tgtccagcgt gttgaatata tccgtgggcc gcgctccgcc
    12961 gtttatggtt ccgatgcgat aggtggggtg gtgaatatca tcacgacgcg cgatgaaccc
    13021 ggaacggaaa tttcagcagg gtggggaagc aatagttatc agaactatga tgtctccacg
    13081 cagcaacaac tgggggataa gacacgggta acgctgttgg gcgattatgc ccatactcat
    13141 ggttatgatg ttgttgccta tgggaatacc ggaacgcaag cgcagacaga taacgatggt
    13201 tttttaagta aaacgcttta tggcgcgctg gagcataact ttactgatgc ctggagcggc
    13261 tttgtgcgcg gctatggcta tgataaccgt accaattatg acgcgtatta ttctcccggc
    13321 tcaccgttgc tcgatacccg taaactctat agccaaagtt gggacgccgg gctgcgctat
    13381 aacggcgaaa tgattaaatc acaactcatt accagctata gccatagcaa agattacaac
    13441 tacgatcccc attatggtcg ttatgattcg tccgcgacgc tggatgagat gaagcagtac
    13501 accgtccagt gggcaaataa cgttaccgtt ggccacggta atattggtgc gggcgttgac
    13561 tggcagaaac agactacgac gccgggtact ggttatgttg aggatgggta tgatcaacgt
    13621 aacactggta tctatctgac cggattgcaa caagtcggcg attttacgtt tgaaggcgca
    13681 gcccgcagcg acgataactc acagtttggt cgtcatggaa cctggcaaac gagtgccggt
    13741 tgggaattca tcgaaggcta tcgttttatc gcttcttatg gcacgtcgta caaggcacct
    13801 aatcttgggc aactgtatgg tttctacgga aatccgaatc tgaatcctga gaaaagcaaa
    13861 cagtgggaag gcgcgtttga aggcttaacc gctggggtga actggcgtat ttccggatat
    13921 cgtaacgatg tcagtgattt gatcgattat gatgatcaca ccctgaaata ttacaacgaa
    13981 gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt ttgataccgg accactgacg
    14041 catactgtga gttatgatta tgtcgatgcg cgcaatgcga ttactgacac gccgttgtta
    14101 cgccgtgcta aacagcaggt gaaataccag ctcgactggc agttgtatga cttcgactgg
    14161 ggtattactt atcagtattt aggcactcgc tatgataagg attactcatc ttatccttat
    14221 caaaccgtta aaatgggtgg tgtgagcttg tgggatcttg cggttgcgta tccggtcacc
    14281 tctcacctga cagttcgtgg taaaatagcc aacctgttcg acaaagatta tgagacagtc
    14341 tatggctacc aaactgcagg acgggaatac accttgtctg gcagctacac cttctgaacc
    14401 acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct atgacgagat
    14461 ccggcatctc ttaccggatc tccattacat ttatgctttc gataacgtcg ctttcccgta
    14521 tggcgaaaaa agcgaagcgt ttattgttga gcgagtggtg gcaattatca ccgcagtgca
    14581 aaaacgttat ccccttgcgc tggctgtggt cgcttgcaac actgccagta ccgtttcact
    14641 tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc
    14701 tgctgcacgt ctgacggcaa atggcattgt cggattactg gcaacccgcg gaacagttaa
    14761 acgttcttat actcatgagc tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct
    14821 gggctcggca gagatggttg agttggctga agcgaagctg catggcgaag atgtttctct
    14881 ggatgcgcta aaacgtatct tgcgtccgtg gttaagaatg aaagagccgc cagataccgt
    14941 tgtactgggg tgtacccatt tccctctact acaagaagaa ctgttacagg tgctgccaga
    15001 gggaacccgg ctggtggatt ctggcgcagc gattgctcgc cgaacggcct ggttgctaga
    15061 gcatgaagcc ccggatgcaa aatctgccga tgcgaatatt gccttttgta tggcaatgac
    15121 gccagaagct gaacaattat tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa
    15181 actggcagtt ttagactgat ttggttgaat gttgcgcggt cagaaaatta ttttaaattt
    15241 cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg aacaacggca
    15301 aacacgccgc cgggtcagcg gggttctcct gagaatctca acagagaaaa gcaaaaaaat
    15361 gcttgactct gtagcgggaa agcgtattat gcacaccccg cgccgctgag aaaaagcgaa
    15421 gcggcactgc tctttaacaa tttatcagac aatctgtgtg ggcactcgaa gatacggatt
    15481 cttaacgtcg caagacgata aatgaat
//
LOCUS       DAOTRK010000112        15495 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.112, whole genome shotgun sequence.
ACCESSION   DAOTRK010000112 DAOTRK010000000
VERSION     DAOTRK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.112"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            3..364
                     /locus_tag="QIE41_003385"
                     /pseudo
     CDS             3..364
                     /locus_tag="QIE41_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349338.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            469..1311
                     /locus_tag="QIE41_003386"
     CDS             469..1311
                     /locus_tag="QIE41_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDS3532237.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCKDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1339..1808
                     /locus_tag="QIE41_003387"
                     /pseudo
     CDS             1339..1808
                     /locus_tag="QIE41_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            2332..2811
                     /locus_tag="QIE41_003388"
     CDS             2332..2811
                     /locus_tag="QIE41_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDS3532238.1"
                     /translation="MKCFLFILIANVFFFSCCSYGGEIPPPIKLHNIEISSTSESFNL
                     SENQRYELDIKSEGGDGDASYRLHQYYTYVCFNIPKQIKYLARSASQGNIIAQYNYAM
                     ILSSEEDDSYLKYYNLDNAINWLKMAASHGYIKAEVELLRLERLKETIRLGKDHPMP"
     gene            2877..3005
                     /locus_tag="QIE41_003389"
                     /pseudo
     CDS             2877..3005
                     /locus_tag="QIE41_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3141..3530
                     /locus_tag="QIE41_003390"
                     /pseudo
     CDS             3141..3530
                     /locus_tag="QIE41_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(3729..4853)
                     /locus_tag="QIE41_003391"
     CDS             complement(3729..4853)
                     /locus_tag="QIE41_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3532239.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(4853..7588)
                     /gene="rbbA"
                     /locus_tag="QIE41_003392"
     CDS             complement(4853..7588)
                     /gene="rbbA"
                     /locus_tag="QIE41_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDS3532240.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPTRCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSSEAEAPPVVHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSALALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(7585..8652)
                     /locus_tag="QIE41_003393"
     CDS             complement(7585..8652)
                     /locus_tag="QIE41_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDS3532241.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(9018..10640)
                     /locus_tag="QIE41_003394"
     CDS             complement(9018..10640)
                     /locus_tag="QIE41_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDS3532242.1"
                     /translation="MKLGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSFQDDKISFTIETNHSEKYSMLSGMRAHILRR
                     NNSYQFIVTINSKNYGCPLDDTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLGQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNKNGLMPLIRRRTMLQL"
     gene            complement(10902..12491)
                     /locus_tag="QIE41_003395"
     CDS             complement(10902..12491)
                     /locus_tag="QIE41_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDS3532243.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKHRSKIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKLWFYNSEPSIDIKEYNSFADNT
                     TDTFIFTIIPDNNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIIERNF
                     FTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIRGEGWSLLCDLLKHEDMASFAYENKKLKKLFTLLDTRD
                     MILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYDLIKTLDVCNYDSETKVLTSHHGI
                     IRNEENECYCLGALQIPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAIAST
                     PVYDNYFNNTTAFRPKPEDIFKCGQTLKKSKQKYGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQSH"
     gene            12874..13926
                     /locus_tag="QIE41_003396"
     CDS             12874..13926
                     /locus_tag="QIE41_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDS3532244.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGIPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIILGSITVLLGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(14241..15443)
                     /locus_tag="QIE41_003397"
     CDS             complement(14241..15443)
                     /locus_tag="QIE41_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDS3532245.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT         4223 a         3595 c         3467 g         4210 t
ORIGIN      
        1 acatgacaac gtataagaaa atattattca gaacctatgt gattgtatct ttgtttttac
       61 atacaacaca tgggcgcagt gtaataatat tattataatg cgcaagtatg aatgtgaggg
      121 gaaatacacc gttagaaatt tggtaaaaaa taaagctata gcattggaat tagctgagat
      181 gtatgttaaa aatcgttatg ggcatgatgc cgcagaagaa gaaaaaccat acaaaatcat
      241 ggaattacca acaagttgga ttgttgaagg caccattgaa tcagaccaaa ttgctggtag
      301 ggtttttatt atagaaatag gcaaaaatga cgggagaatt ctaaattttg gccacggaaa
      361 ataagtatgt aaggataaac aaacatcgtg tgtgctgatg gcagagtagg tggaggactc
      421 cagacagtca aacgatagaa aaagatagtc tttatggagg ttcctgcaat gtcaaataca
      481 taccagaaaa gaaaggcaag taaagagtat ggtttatata atcaatgtaa gaaactaaat
      541 gatgatgaat tatttcgctt acttgatgat cacaattcct tgaaaaggat ttcatctgcc
      601 agagtattac agttaagagg tggtcaagac gctgttagat tggcaattga gttctgctct
      661 gataaaaatt atatccgtag agatatcgga gcatttatac tcgggcaaat aaaaatttgc
      721 aaaaaatgca aagataatgt gtttaatatt ttgaacaata tggcattgaa tgataagagc
      781 gcttgcgttc gagctacggc aatcgagtca acggctcagc gatgcaagaa aaacccaatt
      841 tattcaccta aaatagtaga acaatctcaa attactgctt ttgataaatc gactaatgtc
      901 agacgtgcta cagcatttgc tatttctgtt atcaatgata aagcaacaat tccactattg
      961 attaatctgt taaaagatcc aaatggagat gtcagaaact gggccgcatt tgcaataaat
     1021 atcaataaat atgataatag tgatattagg gattgttttg tggagatgct tcaggataaa
     1081 aatgaggaag tccgtattga agcaataatc ggactttcct acagaaaaga taaaagggtt
     1141 ttatctgttt tatgcgatga gttaaaaaaa aatactgttt atgatgatat cattgaagct
     1201 gcgggtgaat taggtgataa aacgctactt cctgttttag atactatgtt gtacaagttt
     1261 gatgacaatg aaattataac ttccgctatt gataagctga agcgttcata gcgtgagttg
     1321 cctatgcaca gtgggggatt cccgccggca cggtgcagat gctcgaccgg ctggaaagtg
     1381 aaatcctggc tgaccgggtg agtgaggaaa gccgccgctg gctggcatcg tgcggcctga
     1441 cggtgaagca gatgcaaaac cagatggacc cggtgtacac gccggcgcga aaaatccacc
     1501 tgtaccactg cgaccatcgc ggcctgccgc tggtgcttat cagcacggaa ggggcaacag
     1561 aatggtgcgc agaatacgat gaatggggca acctgctgaa tgaagagaac ccgcaacagc
     1621 tgcagcaact catccgcctg ccgggtcagc agtatgatga ggagtccggc ctgtattaca
     1681 accgccaccg ctattatgac ccgctgcagg ggaggtatat cactcaggat tcgattgggc
     1741 tgaagggggg atggaatttt tatgagtatc cgttgaatcc ggttcagtat atagaccctt
     1801 ttggattagc tgatatgaat ttctatgatg aggggatacg cagaaatgta tcactgcatg
     1861 ctgcaacaga taaaataaat attcctggcg tttttactat tggaggacat ggatatcctg
     1921 gttttattac agatgaaaat ggaaactatg aaactgctga gggattagcc aagagaatca
     1981 gagctaatcc acgctataaa aagggaatgt ctgtttgatt attctcatgt ggtacagcca
     2041 gagagggaga taaatctttt gccagcaagt tagccagagc acttcatgca aatgtcactg
     2101 gagctgatac acaatggcaa gtatggatgg cgtgggataa taatactcct ctttttaata
     2161 cgatccaaac aggacttacg gataatatct cggcaattga cacaagtaat aatgtcataa
     2221 ctggtaataa tatgggccgc tggataacgt ttggaccttc aggtcaaccc atttcttcca
     2281 ctcctggcaa agttatgaga ccaagtgata taaaataaca aaggtggcaa catgaagtgc
     2341 tttttattta ttctgattgc aaatgttttc tttttctctt gttgttcata tggaggtgaa
     2401 atcccccccc ctattaagct ccataacatt gaaataagct caacatctga atcgtttaat
     2461 ttaagtgaaa atcaaagata tgaacttgat attaaatctg agggcgggga tggtgatgcg
     2521 tcatatcgat tacatcaata ttatacatat gtttgcttta atatacccaa gcaaattaaa
     2581 tatctggcaa ggtctgcatc tcaaggaaat atcatagcgc aatataatta tgctatgatt
     2641 ctatcctcag aggaggatga ttcctattta aaatattata atttagataa tgcaattaat
     2701 tggctgaaaa tggctgcctc acatggctat attaaagccg aggttgaatt gctaaggctt
     2761 gaaaggctaa aagaaactat aaggttaggg aaagatcatc caatgccgta ggcacaaaga
     2821 tgcaaggcaa gatatttttc atattataag cataatgttg tgaaaagcgg ggctgcgatt
     2881 gtattaaagc catttttctg gcttctgaag gctgaaattc agtcatgatt actcaggctc
     2941 tgcgcctcca tcagaccacc gttgatcacc atatccatgt gtttatgaat aaagggcagg
     3001 gaaagatcac gcaacgctgc ttttttgtac taaataattc gcactaaata ttcaataatt
     3061 gagatattcc ttattaccta aagctgtttt ccattgctta tacatgatca aatactcctt
     3121 acataattaa ggagaacaaa atgaaactta aaaaattgat ggaacatatt tctattatcc
     3181 ccgattacag acaagcctgg aaagtggagc ataaattgtc agatattcta cttttgacta
     3241 tttgtgccgt tatttctggt gcagaaggtt gggaagatat agaggatttt ggggaaactc
     3301 atctcgattt tttgaagcaa tatggtgatt ttgaaaatgg tattcctgtt cacgatacca
     3361 ttgccagagt tgtatcctgt atcagtcctg aaaaatttca cgagtgcttt attaactgga
     3421 tgcgtgactg ccattcttca gatgataaag acgtcattgt aattgatgga aaaacgctcc
     3481 ggcactctta tgacaagagt cgccgcaggg gagcgattca tgtcatttct cgcgtcagtc
     3541 cttgcgggga gcaggctttc gtaatcttgc cctgaatacc ggactgagta tcaaaaagcc
     3601 ggttaactga aactgtccag gttttggtgg tcagttcata aacgccttat ccggcctaaa
     3661 aaaaactaaa attcaataaa ttgcaccaat gcgtagaccc tataagctta cgcatcgggc
     3721 aactgtaatt acgccattgt cccaatcgtc ttcctaaatc gcagcagtgc gatggtgaaa
     3781 aacgcgccgc caattgccat cagcgtcagg aactgcggcc agacgatttc gaatccggca
     3841 ccccggtaga ggatggcctg cgcgaggcta acaaagtgtg tcgtcggcat ggtcagcata
     3901 atgtcctgca ccatctgcgg catactttcg cgcggcgtgg atccaccgga aagcatttgc
     3961 agcggcagca gcaccagaat caccagcagc cccagttgcg gcattgaacg cgctatcgtc
     4021 cccataaaaa tgccgattga cgtggtggca aacagactga gcgccacgcc cagcataaac
     4081 agcgggatcg agccttcaat cggtacgccc agcacgcctt ttaccatcag caccagcgat
     4141 aatcccgata ccaccagcac caccagcccc atcgaccaga tcttcgccat catgatctca
     4201 aacggcgtta tcggcatcac cagtaagtgt tccaccgtgc cgtgttcacg ctcgcggatc
     4261 agcgccgatc cggtcaatac aatcgccagc atagtaatgt tgttgatgat cgccatcacc
     4321 ccgccaaacc acgcgggatc gaggttcggg ttaaagcgca tccgggtttc cagcgatacc
     4381 aacggttcgc tattatcacg gtagcgcgcg acaaagctgt tcacttcacc gttgataata
     4441 ttctggatat acccattgcc ggtaaatgcc tggctcatgc gcgtggcatc gacgttcacc
     4501 tgaatatctg gctggcgtcc ggcgaggaca tcacgctgaa aattaggcgg aatatttatc
     4561 gcgaaggtat agcgtccggc gtccagcccg gcatccatct catcggcggt gatcatctcc
     4621 ggtggcaaaa accacgggcg atagaagctg ttaacgatcc tgttcgataa ctgcgattga
     4681 tccatatcgg caatggcgat cggcgcgagg ttcaacgatc ctggcgtaac ggtcgctgac
     4741 gaatacaccg acaccgtaaa cgagaagaca atcagcgtca gcatcgcttt atcaccgagc
     4801 aaactgcgca actctttgat acccagatta aaaatattgc gtaaatggcg catcatccct
     4861 cctgtttttt cagcagcagg atgcttaagc ccatcaccag cgggatggct atcagcaacg
     4921 ggataaaaag ttgccacaaa tcagtcaaat ccagcgcctt cgagaacgtc ccgcgggcaa
     4981 tagtcagaaa atgactggtc gggtaaacct cgccgatcca gcgtcctgga ccttccagcg
     5041 aagctaccgg atcaatcatc ccggaaaact gtgtcgccgg gatcaacgtg ataatcgccg
     5101 taccgaaaat agcggcaatc tggcttttca taaaggtgga gatcagcagc cccattccgg
     5161 tggcgatgat gatatacagc agcgccgcca gggtgagcgt caggaaactg cctttatgcg
     5221 gtacgccaaa cacaaacacc gacaggccgc agagcaggaa aaagttcagc atccccagcg
     5281 cgatgtacgg tagctgtttg ccaagcaaaa attcgctacg ggtggtggga gtcacgtaaa
     5341 ggttgataat cgaccccaac tctttttccc gcaccacgct aagggcgctt agcattgacg
     5401 ggatcatcat cagcagaagc gggatcaccg ccggaacaat cgctggcagg ctttttacgt
     5461 ccgggttata gcgatagcgc gtctcaatgt tcatcagccc gctttggctg gagggtgtgg
     5521 attgtcggct cgccacatcc tgtagccagc tctggtgcat agcctgcacg taacctttta
     5581 ccgtttcggc acgactgggc atcgcaccgt cgatccagac accgagttcc acaggcgtac
     5641 cacgcgcgat atcgcgcccg aaattgggtg ggatctcaat cgccaccgtg atatcgcccg
     5701 cacgcatccg gcgatccagt tcgtcataac tggtgagcgg tggttgttcg ataaagtaac
     5761 gggaaccgga gagattgagc gtccacgcct ggctactgac ggtctggtcg cggtcaagca
     5821 ccgcaaagcg caggttttcc acatccatac tgatgccgta acccattatc agcatcagga
     5881 tcaccgttcc catcagcgcc agcgccgaac gtaccggatc gcgtcgcagt tccagtgctt
     5941 cgcgacggct gtagctaaac agacggcgca gactaaatcc ctgacgcggc gcgtgggtgg
     6001 cgtcgtgtac cacgggcggc gcttcggctt cgctgctctg ccctgccgct tcctgcaaat
     6061 aggcgataaa tgcctcttcc agactggcgg ctccgcgttt ctcaaccaac tcctgcggtg
     6121 taccgctggc gagcactttt ccggcgtgca tcagtgagat acggtcgcaa cgttccgctt
     6181 cgttcataaa gtgggtggag atgaagatag tcactttgtc ctgacgcgag agatcgacca
     6241 tcaactgcca gaacatatcc ctcgccaccg gatcgacacc agaagtaggc tcatcgagga
     6301 ttaacatctc cgggcgatga atcaccgcca ccgccagcga aagccgctgg cgaatgccga
     6361 gcggtaatga ctccggcaga acatcttcaa cgtcgttgag cttaaaacgc tcgctcattt
     6421 cagccactcg cgcgggaatt tccgcttccg ggatgtgaaa caaacgggca tgtaactcaa
     6481 ggttttgccg cacggtgagt tcgttataga gcgaaaacgc ctgcgacata tagcctaccc
     6541 gacggcgagt atcgatatct tttggatcaa ccggttgccc gaacagccac gcctcacctt
     6601 cgctggcggg cagcaatccg gtaagcattt tcatggtggt ggatttaccg cagccgttcg
     6661 aaccaagaaa accaaaaatc tccccgcgtg gaatgcggaa attgacgtga tcaacggcaa
     6721 cgaaggaacc aaaacgcatg gtcagatcgc gcgcttcgat ggcaatctct gcgttttcag
     6781 gttgatatgg tgggatcact accgcctgat gcgcctggcg ttgcgcttgc ggtaacagat
     6841 ttataaatgc ttcttccagc gtggcgcttt gcgtttgctg ccgcagctct tcggcgctgc
     6901 cggttgccag cacttctccg gcattcatcg ctaccagcca gtcgaagcgt tcggcctctt
     6961 ccatgtaggc ggtggcgacc agcacgctca tattgctctg ccgctggcga atactgtcga
     7021 tcagatccca gaactgggcg cgggagagcg ggtcaacccc cgttgttggc tcatcaagaa
     7081 tcaacagttc cgggtcgtgg attaacgcac agcacagccc cagtttttgc ttcatcccgc
     7141 cggagagttt ccctgccgga cgatcacgaa acggtgctaa cccggtgctg gtcagcagct
     7201 cattaattcg cacttctcgc tccgctttgt cgtgaccgaa caggcgggcg aaaaaatcga
     7261 cgttttcata caccgacaag gtgtggtaga ggtttttacc cagcccctgc ggcatccagg
     7321 cgatgcgcgg gcaaacgtcg cgtcgatgct tcgggtcgcg catatcgccg cccagcacga
     7381 tcacattgcc ctgctcaatg acacgagcac cggaaatcaa tgacaataaa ctcgacttcc
     7441 cgacgccatc cggaccaatc agcccaacca tgcagcgggt cggaatatcg agagtgatat
     7501 tgttcagcgc aacggttttt ccataatgct ggctcacgcc cgccagttgc gcgacaggcg
     7561 ggacgggaac cagttccaga tgcgtcattg cggcaacctc accaccaggt cgtcaggcca
     7621 cggaagttct tcattcaccc gcacccacgc tacgcccggc aaaccggttt tgacatattc
     7681 cagatgctgc tggagtaatt ccggtgggat acgcgctttg acgcggaaca tcagtttcag
     7741 ccgctcatcg ctggtttcga cggtttttgg cgtgaactgg gcgacgctgg cgacaaaact
     7801 gatggttgca ggaatacgca gatctggcgc ggcatcgagg atcagccggg cttcaccgcc
     7861 cagtttcagc gtgcccgcct gttcggttgg caggaagaag gtcatataga cgtcgctcag
     7921 atcgaccata ttcagcaccc gaccgcctgc cgccagcact tcccctggct cggcaacccg
     7981 atactgcacg cgtccgtcac gcggggcttt cagttcgctg tcatcgatat ctgcggcaat
     8041 gcgccgttca gtggcttgtg cggcttcgac gcgtgtttgc gcctgaatga tattggtgcg
     8101 tgccgcttct atagcggctt tagaagccga aacctgggct tttgctgatt ccagcgcagc
     8161 tcgggcgctc tcagcggcgg cgcgatcgtc atccagctgt tgcgcagaaa tagcccctcg
     8221 ttgggcgagc gaacgggaac gcgtatgacg tttggcaacg gagtccagtt cggcctggcg
     8281 ttgattgacc agcgactgtg cggcacgagt ttcgctttgg cgttgctcca gcaaagcctg
     8341 cgcggcggca acggcgcttt gtgcttcttt gatttgcgcg atggcttcca gtcgctgttc
     8401 ctgcaacacg cgagtatcca tcttcgccag cacttcacct tcgcgaacaa actggccttc
     8461 tttcaccaga atggtgtcga tacgcccggc aattttgctg gcgatatcca cttccgtcgc
     8521 ttcaatgcgc ccattgctga cggcaaagcc ttccggcaca cccgctgggc gcaacagcca
     8581 ccacgccacg atagcggcca ccgccagtaa cccgacaacc caccacgcca gatggcgctt
     8641 actcttatcc ataatcgacc cgccataatc cctgtaagca aacgacgcgt gttacaactg
     8701 ccgccgaacg acgttcagcg gattcttcca tccgggataa gaaggaaacg gcaaatacga
     8761 ccagcgcgca gagcattgag cgccaacgtg gaaggcttag tgaaaacggg tagtaatgtt
     8821 gtcatcatca gcattcctgg ccgtaaatga aacacgcata ctcataagtg tgatctataa
     8881 aactggctga caaaacggta tccatcctgt tggatagcgt ttcacgcctg ttcagttgca
     8941 ggaacagagc attccttatt acgttttatc aacagtctca accagatagt aacattaata
     9001 ttcatgttgt ctatggttca gagctgtaac attgtgcgcc ttctaatcaa aggcataagt
     9061 ccatttttgt ttttttcatg ctggtaagac ttccagggag tggttatctc aggccccgca
     9121 ccagggttaa tttggcaatt atgcatgctg aacattcttc cagcatgttt actgtcacgg
     9181 gcgttaagac ctaaaactgt ccccgtttca cctatgcgtt ctctcccacc atttccgtta
     9241 tgtgcaacaa cttgatttat acctaacatt tgtgagcatg ctaatagtgc ttcaagttta
     9301 ggtctgtaat ctgtcgaaac agaaaaataa cgattaaagg atcgacaaga gatagcccgg
     9361 ctagttttaa actttttacc attaattata gtatgatgct ttttattaat aattgccgct
     9421 aattcgaccg gatcgagagc attggtcatt tcatcaatag agactgttat cggtcctata
     9481 atgtaacgtt ttcgctgatc atcaaaagtt atcccatgat gatttgccat tattttgtat
     9541 ttagggtcat agaaacatac aggataatct tttatcccag cgtaggtatc ctcatcaagt
     9601 ggtttcatac gcgtgaagtt ctgcatatag ttaccattgc aattcgtttc atgatttcca
     9661 gctaatacat gaacgttttc attgatactc attatacgtt ttaacaaatc gatgataaat
     9721 tgatcgccac agatattact aaatcgatcg ccggtttggt cacccagaaa cgtacatggc
     9781 gtcacagcgt ctttattaat aactaaataa ggctgaagac tttctaaaac tatatctctt
     9841 ctgttcagaa tactgattat tgtattaaca ttagtgtcaa gttgctcatt aaagttctcc
     9901 cttagcaata cgttgagaac ttcatcttct atattaagca actggccaag gaaattttct
     9961 ccttcagcca tgattcccaa atgtcccgtt gcgaaaagcg cgtacaaaac tgcaccaact
    10021 gagccatcgg tatcgccaaa ataggttggt ctggtacttg tgggatcaac aggtgaacaa
    10081 ttggagaaat aggttctgtc agcgaactgg ggttttatca ttgtttcgaa tttaatatca
    10141 gatacattgt attgcgatat tttccagttc cagatgggtt tgtacgactc agtaactgca
    10201 acatcattag cattatcatt tactgtcata ttattaagta aataaacaat gctgcaccaa
    10261 ttaatatcgg tatcatcaag agggcaaccg taatttttac tattaattgt cacaataaac
    10321 tgataactat tattcctacg tagaatatgg gcacgcatgc ccgatagcat actatatttt
    10381 tctgaatgat tcgtttctat ggtaaatgaa attttgtcat cttgaaaaga ttgttggaaa
    10441 cgttctattt gccaatcatc aattttttcc acaacttcac cgagcattgt cctggcaata
    10501 cttgatatat catcacctat accatttgca atatacccaa acagttcttt agtgaagtta
    10561 gtaacatgct cttgcaccat atcatttgtt gcgattgtcg ttgtactgtc gttagtgcct
    10621 gccacagttc caagtttcat cttttacctc atataattca ccgcaaggaa tacatagtta
    10681 tcatttaatc ttatgacata cacctcatgt tcgacgggga aataattata ttttgcacaa
    10741 atcattcagc cttaaagcat aatatgaaat ttcttatctc atagtagaga aagtccctaa
    10801 agattgtaga ggcgtcatca gtacatgcct ctacaatcga ttaagaaaaa ctatgaaata
    10861 ctggcaggat taaaaaaata taccaccgta ttcatggagt tttaatgaga ttgaaaacaa
    10921 ctaagccctg ggacaccttt tttatcacca cgttcgttag gtgctatctt ccaggaattt
    10981 agccccatgg tataattatc aattttttga tgctgatcta cccctaatcc ataatggcca
    11041 tatttctgtt tacttttttt gagcgtctgt ccacatttga agatatcttc aggttttggt
    11101 ctgaaagcag tagtgttatt aaaataattg tcatatacag gtgtggatgc tatagcattg
    11161 cttctaatta gatggataag acgatcatcc atgtgttgtt tatgtttttt attaaatata
    11221 tttgctaatt cctcaggatc aattgggtct ttcatctgat taaatggaat ctgcaaagca
    11281 cctagacaat aacattcatt ttcttcattt cttattattc cgtgatgact ggttaaaact
    11341 ttcgtttctg agtcataatt acaaacatcg agcgttttaa ttaagtcata tgtatcaccg
    11401 gaagagagtt tatgatttgc tagtcgtgca ctatagttac cattaaaatt gatttcatga
    11461 tttccagcca gaacgacgac atttttattt atacgactat ctttattacc ctccatattt
    11521 cgcatagaat ttagcaaagt aaggatatat ttatcaccaa aaatagtact aaatctatcg
    11581 cctgtatgat ccccaaggaa gatacaaggg gtaatcgcat cgccttttaa aaaaacatgt
    11641 tgatgcaact cattcaagat catgtctctc gtatctaata aagtgaagag ttttttcaat
    11701 tttttatttt catatgcgaa tgaagccatg tcttcgtgtt ttaataaatc acataataaa
    11761 ctccacccct ctcccctgat gcctatatgc cctgaaacga gcaatgcata aagtacagcg
    11821 cctactcttc catctgtatc gccaaaataa gccgtgttgg cattttgtgg attaatcaca
    11881 ccacactgtt tataaaagtc tatttttgaa aaaacagatt caacatagct atcaaacttt
    11941 tccttagtaa acgtctcttt agagacagcc caacgccagt caggtttgaa attaccacta
    12001 agaagagtaa agaaatttct ctctatgata gaaaacccat caactttata aattatgtct
    12061 gatttatctc cggagaaatt aataaaataa taaccgtcct tacgctcaat cgtaacggta
    12121 atgggagacg ataattttat cacatggtta ttgtctggta taattgtaaa gataaacgtg
    12181 tcagtagtat tatcggcgaa actattatat tctttaatat ctatagatgg ctcactattg
    12241 taaaaccata gtttttctat ctgaggacta tgttcagtta cgttccagca gccatgttgc
    12301 aggaaataga tgatatgcag gccaaaatcg ttatctattt gcgaatgttt atccagctta
    12361 tcaatttcaa ctatctttga acgatgtttt ttatcaacct tgccatggtg ttgatgactg
    12421 ttaactttat ctgaatgctt atcttgatgg tttgaagaga agtgcttaaa tatattgtga
    12481 aatactttca taaataaccc gtgatatttt tgacttgtta gtaacaaaat aaatgacaca
    12541 tgtctgaaag acatatttac gatgatacaa agcaataata cttatatgtt cgatgatttt
    12601 aagcaccgat accatccggt cttttaccaa ataatgaaaa agaatccctc cctggaagat
    12661 aattgtaaaa aacaacagaa taagaattaa tcttaggata aatatttatt taccatggcc
    12721 ttttgaacgc tgaaaagtag cgctctgtaa agtaaatccc gctatagtta tgctatgcat
    12781 ttgtctttaa tgatatctcg tcattatatt aacaggctga aattatcatt aatgcacatt
    12841 tcaatattag cagggatacc tgaggagttt aaagtgaaca tatatatcgg gtggcttttc
    12901 aaattaatcc ctttaattat gggcttaatt tgtatcgcgt taggtggctt tgtgctggaa
    12961 agttctgggc aaagtgagta tttcgtcgcg ggtcatgtgc tgatttctct ggcagccata
    13021 tgcctggcat tattcactac cgcatttatt atcatttcgc agctcacgcg cggcgttaat
    13081 acgttttaca atacattgtt ccccattatt ggctatgcgg ggtcaattat caccatgata
    13141 tggggttggg cactgttagc aggcaatgat gtgatggcag acgagtttgt tgccggccat
    13201 gttatttttg gcgttggtat gattgccgcc tgtgtatcga cagtggcagc gtcatccggt
    13261 cactttctgc tcattcccaa aaatgcagca gggagcaaga gcgatggaat accggtacag
    13321 gcttattcgt cattaatcgg taattgcctc attgccgttc ccgtgttact caccctgctc
    13381 ggtttcattt ggtctattac gctgttacgt agtgccgaca taactccgca ttatgtcgcc
    13441 ggtcacgtat tgcttgggtt aaccgcaatc tgtgcctgtc taattggcct tgttgccaca
    13501 atcgtccatc aaacacgtaa tacgttctca tccaaagaac actggctgtg gtgttattgg
    13561 gttattattc tcggctcaat cacggtgctg ctgggcatat acgtgttagt cagctccgat
    13621 gcaagcgccc gactggctcc cggcattatc ctcatttgcc tcggaatgat ctgttacagc
    13681 atattctcaa aagtctggct actggcactg gtctggagac gtacctgttc gttagccaac
    13741 agaataccga tgattcccgt cttcacctgc ctgttttgcc ttttcctggc atcgtttctt
    13801 gcggaaatgg cgcagaccga tatgggatat tttattcctt cgcgggttct ggtcggtttg
    13861 ggagcggtat gctttacgtt gttctcaatc gtttcaatat tagaagcggg ttctgctaaa
    13921 aaataattgc aacgtaccgg ataaaaccag cgttgattat ttgcgtaacg ctggtttttc
    13981 ttaggtatca tgaaataacg cagcattaat gcatagtggt taagtataaa taaacagcta
    14041 agttgctgat tttttcaacc tgttcatatt ttataaagat ctggccactg ggggagtttt
    14101 aatcaccttt ccctccacct tatattaagc atggagggtc tcagttcaca ggctcattag
    14161 aaaataatca caagataacc actatcaaaa ttagctcatt tttaatgcga ctctaataat
    14221 tttcatcttt aggaaatagc tcaggacgac ttcgctgcaa tcaaatcctg cgcacaagcc
    14281 cacgcactcg accacgccca ctggaagtta tagcccccca gccaaccggt gacgtccatc
    14341 acttcgccga tgaagtacag cccaggcact ttgcgcgctt ccatcgtccg tgaagagagt
    14401 tcgttggtgt ccacgccgcc gagcgtcact tcggcagtgc gatagccttc agtgccgttg
    14461 ggttgtacgc gccagtcggt caatgtgcta atcagtgcct gttggtcacg cacgttgagc
    14521 tgttttagcg aaacatccgg gatttgcccg agttgttgta aacgttcaac caaccgcttc
    14581 ggtagatgaa ccgccagtgt gtttttcagg ctctgattcg gatgtgcatt acgctgctca
    14641 ttcaggaagg tttcgaggtc cacatccggt agcagattga tactgacaaa ttcacccggt
    14701 tgccagtagc tggaaatttg caacaccgcc ggtccagata agccgcggtg ggtgaagagt
    14761 aagttctcac ggaaaacggt gccgttttca gcggtaatca cggaaggcac cgccacgcct
    14821 gccagcacct gtaactcttc gagcaacggt ttatgcagag tgaatggcac cagacccgcg
    14881 cgggtcggca gcacgttgag gccaaattgt tcggcaatct tataaccaaa cggcgacgcg
    14941 cccagccccg gcattgacag tccaccggtc gcgatgacta gcttttcgca accgacagtc
    15001 atgccgttca gttcaagcgt gaagcctgta tcatccttct ccacgctcag cacttcgcta
    15061 cgcaatctga aggtcacatt gcccttctcg cactcatcca ccagcatgtc gacaatctgc
    15121 tgagcggagt catcgcagaa gagttgccct aacgttttct cgtgccaggc gatgccgtgt
    15181 ttattgacca gatcaatgaa atcccactgg gtaaaacgcg cgagtgcaga cttacaaaaa
    15241 tgcggattct ggctcagata agcgcccggt tcgacataaa gattggtaaa gttgcagcgc
    15301 ccaccgccag acatgaggat tttgcgccct ggttttttac cattatcgat cagcagaacc
    15361 cggcgtcctg cctgacctgc cagcgcagaa cagaacatac ccgccgcacc agcgcctata
    15421 ataatggcat caaacctttc cacgttgcgc tcctctaaga aaaaacgggc gtgaattgta
    15481 aagattcctc agtgg
//
LOCUS       DAOTRK010000113        15411 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.113, whole genome shotgun sequence.
ACCESSION   DAOTRK010000113 DAOTRK010000000
VERSION     DAOTRK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.113"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="QIE41_003398"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="QIE41_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDS3532246.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="QIE41_003399"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="QIE41_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDS3532247.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="QIE41_003400"
     CDS             complement(941..1408)
                     /locus_tag="QIE41_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDS3532248.1"
                     /translation="MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="QIE41_003401"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="QIE41_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDS3532249.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="QIE41_003402"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="QIE41_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDS3532250.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="QIE41_003403"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="QIE41_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDS3532251.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="QIE41_003404"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="QIE41_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDS3532252.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="QIE41_003405"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="QIE41_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDS3532253.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="QIE41_003406"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="QIE41_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDS3532254.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="QIE41_003407"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="QIE41_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDS3532255.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="QIE41_003408"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="QIE41_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDS3532256.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="QIE41_003409"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="QIE41_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDS3532257.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="QIE41_003410"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="QIE41_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDS3532258.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="QIE41_003411"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="QIE41_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDS3532259.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="QIE41_003412"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="QIE41_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDS3532260.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="QIE41_003413"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="QIE41_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDS3532261.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="QIE41_003414"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="QIE41_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDS3532262.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="QIE41_003415"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="QIE41_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDS3532263.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="QIE41_003416"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="QIE41_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDS3532264.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="QIE41_003417"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="QIE41_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDS3532265.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="QIE41_003418"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="QIE41_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDS3532266.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="QIE41_003419"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="QIE41_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDS3532267.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="QIE41_003420"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="QIE41_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDS3532268.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="QIE41_003421"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="QIE41_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDS3532269.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="QIE41_003422"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="QIE41_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDS3532270.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="QIE41_003423"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="QIE41_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDS3532271.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="QIE41_003424"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="QIE41_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDS3532272.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="QIE41_003425"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="QIE41_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDS3532273.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="QIE41_003426"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="QIE41_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDS3532274.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="QIE41_003427"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="QIE41_003427"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDS3532275.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..>15411
                     /gene="rplQ"
                     /locus_tag="QIE41_003428"
     CDS             15058..>15411
                     /gene="rplQ"
                     /locus_tag="QIE41_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDS3532276.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDR"
BASE COUNT         3967 a         3632 c         4104 g         3708 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagtttcgaa ttaaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaatttca ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc tatcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc taaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtctacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccagcc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccatgtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaact ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggta
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctattgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa accaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgtt gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggta
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat tccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct ttctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcatacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgtactgg
    10201 ctaaagcgta tggttccacc aacccgatta acgtggttcg tgcaactatc gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag gggggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaactac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aatcaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gctacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 accatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtaca ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgatttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg t
//
LOCUS       DAOTRK010000114        15398 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.114, whole genome shotgun sequence.
ACCESSION   DAOTRK010000114 DAOTRK010000000
VERSION     DAOTRK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.114"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1..398
                     /locus_tag="QIE41_003429"
                     /pseudo
     CDS             1..398
                     /locus_tag="QIE41_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(503..1552)
                     /locus_tag="QIE41_003430"
     CDS             complement(503..1552)
                     /locus_tag="QIE41_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated sensor domain-containing
                     protein"
                     /protein_id="HDS3532277.1"
                     /translation="MVIRGMTISLPWFAFINVSFALIILLRRVLFNDLTPPWLNEKSL
                     IHSIDISATGILLICSGLLLIPRQKTLPIQVLLVALSLLWSWCSYHFIAYWTLQFAYP
                     LCVLLMLSGVVALYFHTPSLLAFVIPLWFTTPIASLMLNQQINIHFAVVWCIFSLALY
                     GGRLILLRWFEEAWVQNSYNNQLINRLDALAHRDPLTGIANRRAMNSILHDAMDNGGS
                     FALIMLDVDFFKRYNDTYGHPAGDRCLIQVADALQRSTRQSEDVVVRYGGEEFAIILF
                     NATLPEAETVAARVKQELKLAAIPHQASAVNAFVTVSQGITCSAPAKTAEQIISDADA
                     ALYRAKESGRNRWAL"
     gene            complement(1647..3623)
                     /locus_tag="QIE41_003431"
     CDS             complement(1647..3623)
                     /locus_tag="QIE41_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS3532278.1"
                     /translation="MQLNHIVKSLLITGLFTTSSLPLLAAEAPKDATAATQQANNLLY
                     NQLPFADNTDFTDAHKGFIAPIPQDVIKGEQGNVIWDPQHYAFIKEGDKAPDTVNPSL
                     WRQAQLINISGLFEVTEGVYQIRNLDLSNMTIIEGKEGITVVDPLVSAETAKVGMDLY
                     YKNRGKKPVVAVIYTHSHVDHYGGVRGVIDEADVKSGKVKIYAPAGFLEEAVSENIMA
                     GNVMSRRASYMYGNLLKPDVKGQVGAGLGTTTSAGTVTLIAPTNYITKTGQIETIDGL
                     TYDFLMAPGSEAPSEMLWFIEEKKLIETAEDVTHTLHNTYSLRGAKIRQPLPWSKYIN
                     EALNLWGDKAEIILAQHHWPTWGNDNVVKLLKSQRDLYRYINDQTLRMANQGMTRDEI
                     AANFKLPSSLANTWANRGYYGSVSHDVKATYVLYLGWFDGNPATLDELPPEEGAKKFV
                     EYMGGADAILQKATQDYDQGNFRWVAQVVSKVVFADPNNQAARDLEADALEQLGYQAE
                     SGPWRNFYLTGAQELRNGVQKLPTPNTASPDTVRAMTPEMFFDYLAVHINGEKAADAK
                     TVLNFDFGEDGGTYKVELENGVLNHTAGVEASDADATITLSRDVLNKIVLKEETLKEA
                     TAKGDVKITGNEEKLNELLGYMDNFEFWFNIVTP"
     gene            3929..4156
                     /gene="pptA"
                     /locus_tag="QIE41_003432"
     CDS             3929..4156
                     /gene="pptA"
                     /locus_tag="QIE41_003432"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDS3532279.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(4160..4729)
                     /locus_tag="QIE41_003433"
     CDS             complement(4160..4729)
                     /locus_tag="QIE41_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDS3532280.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            4904..5749
                     /gene="nhoA"
                     /locus_tag="QIE41_003434"
     CDS             4904..5749
                     /gene="nhoA"
                     /locus_tag="QIE41_003434"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDS3532281.1"
                     /translation="MTPILNHYFARINWLGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVEVEGEKWIADVGFGGQTLTAPIRLVPELVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHTVEQRNLPDVASLYAVMQEQFGLGVDDVKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            complement(5833..6726)
                     /locus_tag="QIE41_003435"
     CDS             complement(5833..6726)
                     /locus_tag="QIE41_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDS3532282.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGPPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLHVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEF"
     gene            complement(6805..7485)
                     /gene="narI"
                     /locus_tag="QIE41_003436"
     CDS             complement(6805..7485)
                     /gene="narI"
                     /locus_tag="QIE41_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDS3532283.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMIILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            complement(7482..8177)
                     /gene="narW"
                     /locus_tag="QIE41_003437"
     CDS             complement(7482..8177)
                     /gene="narW"
                     /locus_tag="QIE41_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDS3532284.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREASWYALFDTLLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            complement(8177..9721)
                     /gene="narH"
                     /locus_tag="QIE41_003438"
     CDS             complement(8177..9721)
                     /gene="narH"
                     /locus_tag="QIE41_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDS3532285.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRAHDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            complement(9718..13458)
                     /gene="narZ"
                     /locus_tag="QIE41_003439"
     CDS             complement(9718..13458)
                     /gene="narZ"
                     /locus_tag="QIE41_003439"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDS3532286.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKNNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMHYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKIS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKREGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            complement(13553..14941)
                     /gene="narU"
                     /locus_tag="QIE41_003440"
     CDS             complement(13553..14941)
                     /gene="narU"
                     /locus_tag="QIE41_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDS3532287.1"
                     /translation="MALQNEKTSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(15256..>15398)
                     /locus_tag="QIE41_003441"
     CDS             complement(15256..>15398)
                     /locus_tag="QIE41_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDS3532288.1"
                     /translation="RNIKIKNMTLDEINNTYCNNDYYNEAIREEPIDFLDRTFSSSSW
                     PF"
BASE COUNT         3901 a         3869 c         3815 g         3813 t
ORIGIN      
        1 gaaggctggg aagatataga ggattttggg gaaacacatc tcgatttttt gaagcaatat
       61 ggtgattttg ataatggtat tcctgttcac gataccattg ccagagttgt atcctgtatc
      121 agtcctgaaa aatttcatga gtgctttatt aactggatgc ttgactgcca ttcttcagat
      181 gataaagacg tcattgcaat tgatggaaaa acgctccggt actcttatga caagagtcgt
      241 cgcaagggag cgattcacgt tattagtgtg ttctcaataa tgcacagtca gattccttgg
      301 aagatcaaga cgaatgaaaa aaacaatgag attacagctg tttctgaatt tcttaacata
      361 atgggtatta aaggaaaaat aattgcaccg atccaatgtg ataccagaaa gacatcgcta
      421 agaagatagc tatagacaat tattaccctc cgtcagccct tgttaagcga ggagagacgt
      481 aatctgtcca gtcccctaat gcttataaag cccatcgatt tcgcccactt tctttcgccc
      541 ggtataacgc agcgtcggca tctgaaataa tttgttcagc cgtttttgcg ggagcagaac
      601 aggtaatccc ctgactaacc gtaacaaatg cattaacagc agatgcctga tgtgggatag
      661 ctgccagttt aagttcttgc ttcactctgg ctgctaccgt ctctgcttcc ggcaatgttg
      721 cattaaaaag tatgatggca aactcttcgc caccataacg gacaacaaca tcctccgact
      781 ggcgagttga acgctgtaga gcgtcggcaa cttgtattag acaacgatcc ccagcaggat
      841 gcccgtaagt gtcgttgtaa cgtttaaaaa aatcgacatc cagcataata agagcaaatg
      901 acccgccgtt atccatcgcg tcgtgtaaga tactgttcat tgcacgtcgg tttgctatcc
      961 cagttaaagg atcacgatgt gctaatgcat caaggcgatt aattaactgg ttgttataac
     1021 tgttttgtac ccatgcttct tcaaaccaac gtaacaaaat gagtcgccca ccatatagcg
     1081 ccagtgaaaa aatacaccat acaacagcaa aatgaatatt gatctgttga ttaagcataa
     1141 gactcgctat tggagtggtg aaccataagg gaatgacaaa tgcgagcaat gatggggtat
     1201 ggaaataaag tgcgacaacg ccactaagca ttaataatac acatagtgga taagcaaact
     1261 gcagcgtcca gtacgcaata aagtgataac tacaccatga ccatagcagg ctcagggcaa
     1321 ctaatagcac ctggataggt aatgtttttt gccgagggat gagtagtaaa ccactgcaaa
     1381 tcagcagaat acccgttgca gatatatcaa tagaatgtat aaggcttttt tcatttaacc
     1441 agggtggagt aaggtcatta aacagaacac ggcgcagcaa aattatcaat gcaaaactga
     1501 cattgataaa agcaaaccat ggcaaactaa tcgtcatgcc gcgaataacc atatcgcggc
     1561 gggttttcca tgagtgatga gtttttcttc gttcagccat tttttaacca aaaaagggca
     1621 ggatacctgc ccggtatgca gtaaatttat ggtgtcacta tattgaacca aaattcaaaa
     1681 ttatccatat aacctaacag ctcgttgagt ttttcctcat tgccagtaat tttgacatct
     1741 cctttggccg tggcttcttt cagcgtctct tctttcagta caattttgtt caatacatca
     1801 cgagacagag tgatagtggc atcagcatcc gaagcctcta caccagcggt atgattgaga
     1861 acaccatttt caagctccac tttataggtg ccgccatctt caccaaaatc gaaattcaac
     1921 acggttttgg catctgccgc tttctcacca ttaatatgga cggcaagata atcaaagaac
     1981 atctccggcg tcatcgcacg cacggtgtct gggctggcag tatttggtgt tggtaatttt
     2041 tgtacgccat tacgcaattc ctgtgcacca gtgagataga aattacgcca cggcccagat
     2101 tcagcctgat agcctaactg ctcaagcgca tcagcttcca gatctctcgc tgcctggttg
     2161 tttggatcgg caaacaccac cttactaaca acttgggcaa cccagcggaa attaccctgg
     2221 tcataatcct gtgtggcttt ctgtaaaatg gcatcagcac cgcccatata ctcgacgaat
     2281 ttttttgcgc cttcttctgg cggcagttca tcaagcgtcg cggggttacc atcaaaccag
     2341 ccgagataaa gcacgtaagt tgctttcacg tcatgactta ccgaaccgta atagccgcga
     2401 ttcgcccagg tgttagccag tgaagagggc agtttaaagt ttgcggcaat ttcatcacgg
     2461 gtcatccctt gattagccat gcgcagagtt tgatcattga tgtaacgata caaatcacgc
     2521 tggcttttaa gcagtttgac cacgttatcg ttgccccagg ttggccagtg atgttgagcg
     2581 agaataatct cggctttatc accccacaaa ttgagagctt cattaatata ttttgaccac
     2641 ggcagcggtt ggcgaatttt agccccacgc agcgaatagg tgttatgaag ggtgtgcgtg
     2701 acatcttcag cggtttcgat cagtttcttt tcttcaataa accacagcat ttcagaaggc
     2761 gcttcagaac ctggcgccat caggaaatca taagtcaacc cgtcaatagt ttctatttgt
     2821 ccggttttgg tgatgtagtt agtaggagca atcaacgtga ctgttcctgc tgaagtagtc
     2881 gttcccagtc cagcccccac ctggccttta acatcaggtt tcaataagtt gccatacata
     2941 taactggctc gtcggctcat aacattgcca gccatgatgt tttcggaaac ggcttcctcc
     3001 aaaaatccgg ctggcgcata gattttaact ttgcctgatt taacgtcagc ttcatcaatt
     3061 acgccacgaa caccaccgta gtgatcgaca tgactgtgtg tatagataac cgccactacc
     3121 ggttttttgc cgcgattttt gtaatagaga tccataccca ctttggctgt ttctgctgaa
     3181 acaaggggat caacgacggt aataccctct ttgccttcaa tgatggtcat atttgataga
     3241 tcgagattgc gaatctgata gacaccttca gttacttcga ataacccgct gatatttatc
     3301 agttgcgcct gacgccataa actcggattc accgtatcag gggctttatc gccttctttg
     3361 ataaaagcgt aatgttgtgg atcccagata acattccctt gttcgccctt aatcacgtct
     3421 tgagggatag gggcaataaa acctttatgg gcatcagtaa agtcagtgtt atcggcaaac
     3481 ggcaactggt tgtagagtaa attgtttgct tgttgcgtgg ctgcggtggc gtctttagga
     3541 gcttccgccg cgaggagagg taaagaactg gtggtgaata atcccgttat tagcagactt
     3601 ttaacgatat gattaagctg catcttaagc gtctccagaa tggattttta ttgggggtaa
     3661 ttacggatga aaatttttag tgagggcaag aataactata atgggcgtta acaaattggc
     3721 aagccgggaa attgatgttt tcttaatggc atgttggcga agaatatatt ctgcaagtta
     3781 acgttttcat tggcgcttta tttaaaagtg tgtagtacaa atatgtacaa tttcaagtag
     3841 gcattattgc cgtaaaaacg cattaccgga cacacgacat tcacaacgtg tgttaatttt
     3901 actgacatct ttcaaaagga gcacaatcat gccgcacata gacattaaat gttttccgcg
     3961 tgaactggac gaacaacaaa aagcagcact tgctgcagat attaccgacg ttattattcg
     4021 ccatctgaac agtaaagata gctcgataag catttctcta cagcagattc aaccagaatc
     4081 ttggcaagct atctgggatg ccgaaatcgc gccccaaatg gaggctttga taaagaaacc
     4141 cggttacagc atgtaatgct taacctgccg taacacgctt gccgctggta acaaacgtgc
     4201 cagcacctaa atgatgcaac gtattgagtt tgtcatcatc aaactgccag cggccttcga
     4261 caaatactcg tgcgtctgcc gctgcagata ccacttcgcc aaatagcgta tcgtattttt
     4321 cttgcgcgga agttgcaggt agcaggcgac actccatcca cgccagacat ttttcctcga
     4381 ccagcggcaa accaagcacc ggacctttca caaccggaat accatagcaa ttaaacttat
     4441 cttcatcacg ccccgacaca cttcccaccg cccacgtcca gttagttgct gcaacacccg
     4501 ggataacgat gccaaactta ccattacgtt caatcaactc cctggtccat gtcgatttat
     4561 ctaccacaat cgccacgcgt ggcggttcaa actccaccgg cattgaccag gcggctgcca
     4621 taatgttacg ccgctgggat tgctcatcga aactggtgat caagacagtt gggccatgat
     4681 ttaacagacg gctggcatgg tgtaattcga cagggatgaa tcggctcacg gtatcgcctc
     4741 gctatgaagg gtgttttaaa agcgtagctc gtaacgcaat aagtaacgaa attaacggga
     4801 ttggcgattt gcgaacgtga tgcatgtccg cgatcgcaca aaatagccgg tgcagcgtct
     4861 attccaggtt ataagttgag aaaaccacta agggaaacgc ctgatgacgc ccattctgaa
     4921 tcactatttt gcccgtatta actggttggg agctgctgcg gtcaatatag atacgctgcg
     4981 tgcattgcac ctgaaacaca attgcaccat tccgtttgaa aacctcgacg ttttgctgcc
     5041 gagggaaata cagcttgatg atcaatcgct ggaagagaaa ctggtgacag cccgtcgtgg
     5101 tggttactgt tttgagcaga atggcgtgtt tgagcgggtg ttacgcgagc tggggtttaa
     5161 cgttcgcagc ttgttagggc gcgtagtgtt atcaaatccg ccagcattac cgccgcgcac
     5221 ccatcgtttg ttgttggtgg aagtggaagg ggaaaaatgg attgctgatg tcggtttcgg
     5281 tggacagacg ctaaccgcgc cgattcgatt agttcccgag ctcgtgcaga ccacgccaca
     5341 cggagagtat cggttgttgc aggagggtga tgattgggtg ttgcaattta atcatcatca
     5401 gcactggcag tcgatgtacc gttttgatct ctgcgagcag caacaaagcg attatgtaat
     5461 gggcaatttc tggtctgcgc actggccgca gtcgcatttt cgccatcatt tgctgatgtg
     5521 ccgccatttg ccggacggcg gcaagctgac actgaccaat tttcatttta cccattatga
     5581 aaatgggcac acggtggagc agcgaaatct accagatgtg gcgtcgttat atgccgtgat
     5641 gcaggaacag tttggtctgg gcgtggatga tgtgaaacat ggctttactg tggatgagtt
     5701 agcgctggtg atggctgcgt ttgatacgca cccggaggcg ggaaaataat ttatgtcagg
     5761 ttgccggatg cggcgtaaac gccttatccg gcatacatag acctgataag cgaagcgcat
     5821 caggcaacgg tttcagaatt caattgccca ttcggcatga aacaaaatca ccgctgtgcc
     5881 agaaatggtg actttctccg gttggttatc gcgaattgtc accgtcactt caatcacgcc
     5941 gtcacgccct aatgcgcgcc cctgatggcc tttaacatgc aacacgttgc catcgtgggg
     6001 caatacgttg tgatgtacca accatgcacc catcgggcca ttggcattgc cggtcaccgg
     6061 atcttccaca ataccaatgg caggcgagaa catacgaccg tcggtttcgt ttttgcctgg
     6121 gcggatctgg aacgggaaga aaccattgca gccaatctgt ttgctgatag cggtcagcgc
     6181 attaaggtca ggcgaaaggg cgtcgatatc cacttccggt ttcagtggga tcatcacttt
     6241 tgagtgtcct gtggttgcca cctgaattgg caagcctggc agaatatcgt cttcagtgag
     6301 atgaagcgcg ttgataatcg ccgcacgtgt ttcaccttcc agcggcggtt caaagcccgg
     6361 cggaccttgt tccagcgaaa tacgataatc atcgttgtgc ttttcgatag tcacgcgatg
     6421 ttttcctgcc agtgacgttt gccagatcgt gcaatttcct aaacctaaca ccttcgcacg
     6481 tacatagtgc gcagctactg tcgcgtgacc gcaaatcggc acttcaaccg ttggcgtaaa
     6541 gtagcggatg cgtacatcgc tgtcgtcgct gtgcagcaga aaagcagttt ccgaatgacc
     6601 taattcgcgg gcgataagct gcatttgcgc ttcactgaga ttatcggcgg ggaaaacaac
     6661 accggcagaa ttgccgcgaa acggttgtga ggtaaaagca tcgacgtggt acacctgcgg
     6721 tttcattaac gttctcctgt ggctggagaa ctatcatagc ctgcaagtgg ccggagagcg
     6781 aagggctatc cggccagggt gaaattatcg ccgcgagcgc acaatctgat accgacgagt
     6841 aaagtactca aacggcgcgc tccacacatg caccagtcgg gtgaacggga agagcaggaa
     6901 gatggtcatt cccaacacca gatgcaggcg gaaaacgaac gctacgccgt taagcatttc
     6961 tgacgatcca ccacggaaag tgacaatgct ttgcgcccaa cccaccagct tcatcatttc
     7021 gctaccatca ggatactgtg ctgaaaacgg tatggtactt agccccagca gacactgaat
     7081 cagcaaaatg atcatgatga taatatccgg cgtagtggaa gtggcacgca cgcgctgatt
     7141 tgtcagcctg cgccacagca gtcctgcgcc gccaatcagc gtcaatacac cgcaaatacc
     7201 accgagcacc atcgccatct gctgttttac cgccaccggc agaaaccacg cgtacatcca
     7261 gtgcggcgtt aacatgccga acaggtgccc gaagaaaatc cccaaaatgc cgatatggaa
     7321 cagattcgac catatcacca tcccgcgttt atcgagcatc tgactcgagg aggcgcgcca
     7381 ggtatactgc ccgtagtcgt agcgcagcca gctgccgagg aaaaacaccg tcgcacaaat
     7441 gtacgggtag atatcgtaaa aaaagacgtt cagatactga atcatttccc acctcccgca
     7501 ctgatgtcga catactgcgg cgcgacatcc tggctaaagc gtcgctgata ttgattaagc
     7561 ggcgagctgt cacacgccgt ggcgttatct tcaataaact tcacctgttc ctcttcccac
     7621 accgcatcca gtgcctggcg ggtgtcatcg cgctcctcac tgttcacctg ttttgtgaca
     7681 ctgtcacttg aaagtgtact tcctgccagt tgcaacaggg tgtcaaataa cgcgtaccac
     7741 gatgcctcgc gttgttttaa gcgtccgcca agcagggcga ggatcggcgc aacgttgagc
     7801 aagccttctt tcgcctgatc gtccggcagt acgcttaagt actccagata cagcggtaga
     7861 taatcgggca gttctcgaca atccaactgc aggccgacct tttcatactc cgccagcagg
     7921 tccaccattg cctggccgcg atcgcgggac tcggcatgaa catgttcgaa cagcagcagc
     7981 gatgtggtac gtccacggtc aaacacttcg caccattcgg cctgtttatc cagcagcggc
     8041 gcgttaagca ggttgcgggt aaaatccgta agcatcggtg cgtcgcggcg gatcaacgcc
     8101 agcgcgtcct ccttacattc ccacaacagc tcgtctggat actccatcag caggccgatc
     8161 actttgagga tctgcattat tcgccctccg ctttgtcgcg cacttcggtg atgttgatgg
     8221 catcgatacg gctactgttg aacaggttga atttactgtc cgaaccgtgg caaccgtcgc
     8281 caaaggtaaa accgcagcca ttgcgttctg cgaaggcatc gcccgccatt tcccgatggc
     8341 tggtcgggat aacaaaacga tcttcatagt tggcaatggc gagatagcga tacatttctt
     8401 cgacctgggc gacgctcagg cctacttcgt cgatggcacg agtatcagta acgccttcca
     8461 cggtttgtga acgcatatag tggcgcatcg ccatcatccg tttcagcgcc cgcagtaccg
     8521 gaccggtatc gccggcactc aacatattgg cgagatattg caccggaata cgcaggcttt
     8581 cgatggcggg cagcacgcct tcgcttttcg gcaaaccgcc cgcatctgcg taggactgaa
     8641 tcggtgacag cggaggaacg taccagacca tcggcagggt gcggtattca gggtgcagcg
     8701 gtaacgccag tttccagtcc atcgccattt tgtagactgg cgaacgctgg gcagcgtcaa
     8761 tcacattttg tggaatacct tgtttcaggg cttcttcgat cactgaggga tcgtgcggat
     8821 cgaggaacac ttcgcactgg cgttcataga ggtcaacttc gcgctcggtg ctcgccgctt
     8881 cctcgatgcg gtcggcgtcg taaagcagca cgcccagata ccggatgcga cccacgcagg
     8941 tttctgagca cacggtgggt tggcctgact caattcgtgg gtaacagaag atacattttt
     9001 ctgacttgcc gcttttccag ttgaagtaga tttttttgta cggacaaccg ctaatgcaca
     9061 aacgccagcc acggcattta tcctgatcga tcagcacaat gccgtcttct tcgcgtttgt
     9121 agatagcgcc gcttgggcag gtcgccacgc aactgggatt gaggcagtgt tcgcacaagc
     9181 gcggcaggta catcatgaag gtgttttcaa actgcccgta catctccttt tgcatggcct
     9241 cgaagttgcg gtcatgggcg cgtttttcga attcgccgcc caacaattct tcccagtttg
     9301 gcccccagat cactttgtcc atccgtttgc cgtcaatcag tgaacgcggg cgagcagtag
     9361 ggatatgttt gccttccggt gcgctatgca aatgttcgta gtcgaaggtg aaaggttcgt
     9421 agtaatcatc aatctgcggc accaccgggt tggcgaagat tttggttatt acgcccatct
     9481 tgctgcccag acgcgggcgg atcttgccat tcacatcacg cacccagccg ccttgccact
     9541 cttcctgatc ttcccagttt ttcggataac caatgcccgg tttggtttcg acgttgttaa
     9601 accatgcgta ctccatgcct tcgcgaccgg tccagacgtt tttacaggtc accgaacagg
     9661 tatggcagcc gatacattta tcgagattaa gcaccatgcc gacttgcgaa cgtattttca
     9721 ttttttcgcc tcctgtacct gatcgcgacc ttcatcatcc agccagttaa cgttcttcat
     9781 cttgcggatc atgatgaact cgtcacggtt cgagccgacg gtaccgtagt agttaaagcc
     9841 ccaggccagc tgcgcgtaac cgccaatcat atgcgttggt ttcgggcaaa cgcgggtgac
     9901 cgagttatga atgccgccgc gcatgccagt tacttccgaa ccaggaatat tcataatgcg
     9961 ttcctgggcg tgatacatca tggtcatgcc cggcggtaca cgttggctga ccaccgcgcg
    10021 ggccgtcagc gcgccgttgg cgttaaatac ttccacccag tcgttatcga caatggtcag
    10081 ttctcgggca tcggtttcgc tgatccagac aatcggtcca ccgcgagaga gcgtcagcat
    10141 tagcaggttt tcactgtagg ttgagtgaat gccccatttc tggtgcggcg tcaggaagtt
    10201 aagtgctttt tccgggaagc cgtttggcgg gatctggcgc atctcactga cgctacgggt
    10261 gtcgataggc gggcgataag ccaccaggct ttcaccaaaa gcacgcatcc acggatgatc
    10321 ctgatagagt tgctggcgtc ccgacagcgt gcgccacgga attaactcat gaacgttggt
    10381 gtatcccgcg ttataggaga cgtgatcgct ttccagacca gaccatgtgg ggctggagat
    10441 aattttacgt ggctgcgcct gaatatcgcg aaagcgaatc ttctcgtcct ctttgtgcag
    10501 cgccagatgg gtatgttcgc gtccggtgat ctcgcccagc gcctgccacg ctttgactgc
    10561 aacatgaccg ttggtttccg gtgccagagc cagaatcact tctgatgcgt caatggcggt
    10621 gtcaatcagc ggacgcccct gtgccgggcc ttcacgcttg gtgtagttga gtttgccgag
    10681 gaaatcgatt tcatcctggg tattccacga aatcccttta ccgccgttgc caagtttgtc
    10741 catcaatggc ccgagcgagg taaagcgttc ataggtagca gggtagtcgc gttccaccgc
    10801 cacaatattg ggcgcagttt tacctggaat cagatcacat tcgcctttgc gccagtcgag
    10861 cacttcacac ggctgtgaga gttctgccgg agagtcatgc agcagtggtt gtaataccac
    10921 gtcggtttct ttgccaagat ggcccacgca cacttgcgaa aaggctttgg caataccttt
    10981 gtagatttcc cagtcgctgc gtgattccca cgccggatcg accgccgcag aaagcggatg
    11041 aataaacgga tgcatatccg aggtgttcat atcgtctttt tcgtaccagg tggcggtggg
    11101 cagaacgata tcggagaaca ggcaggtact ggacatacgg aagtcgagcg tcaccagcag
    11161 gtcgagcttg ccttcaatcg ccgcagtttg ccactcgact tcttctggtt tgatcccgtc
    11221 gctggcaccg agttcctgac cctgaatccc actttcggtt cccagcagat acttctgcat
    11281 atactcgtgg cctttgccgg aggagccaag caggttagaa cgccagacaa acaaattacg
    11341 cggatgattg ctgccgctat ctggttgttc gcaggccatg cgtaaatcgc ccgatttcag
    11401 cgcctgggcg gtaaattctg tgggggataa tccggccttg tcggcttcag ctttaatccc
    11461 gagcgggtta cgccccagct gcggcgcaga aggtagccag cccatacgtt cggcacgaac
    11521 gttgaaatca atcagatgac cgctgtactt actggcatcg gcgagcggtg aaagtaactc
    11581 ctgagcagag attttttcat agcgccattg gctggaatga ttgtagaaaa acgaggtgct
    11641 gttcatctgg cgcggtgggc ggttccagtc gagcgcaaag gccagcggca accagccggt
    11701 ttgtgggcgc agtttttcct gaccgacata gtgtgcccat ccgccaccgc tttgcccgac
    11761 acaaccgcag aagatcagca tgttgatcat cccacggtag tgcatgtcca tgtggtacca
    11821 gtggttaaca cctgcgccga ggataatcat cgagcgccca tgcgttttat gggcagtatc
    11881 ggcaaattca cgagcgatgg tttcaatata ctggcgcggc acaccggtaa tttgctcacc
    11941 ccaggctggg gtgtacggtt tgatttcagc gtaatctttc gcgctgtttt catcttccag
    12001 cccgcgatcg aggccgtaat tcgccagtac caaatcataa acgctgacca ccggacaggt
    12061 gctgccatcg gctaatgtca ggtttttaac gggtaattga cgcaccagga caggattgtt
    12121 ttttacgctg cgaaaatgcg gattttcaat gccgccaaag taggggaagg ccacgcctgc
    12181 aacggcatca tgttgaccga gcagagttaa cgacaattcg gtttccgtac cggcggcaat
    12241 ggattccaga ttccatttgc ctttttctcc ccagcgaaaa ccaatcgaac cgttcggcac
    12301 caccagttca cctgcggtat taaccgccac ggttttccac tgcggattgt tgctttcgcc
    12361 cagtccatcc accaggtcag atgcgcggac catgcgcccg ggaacgtagc tgccgtcgtc
    12421 gcgaggttcc agcattacca gcatcggcat gtcgctgtag cggcggcagt agttgataaa
    12481 ataatcgctg ggattatcga gatgaaactc ttttaaaatg acatggccca ttgccattgc
    12541 cagggcacta tcagtgcctt gtttcggtgc cagccactgg tcgcacaatt tggccacttc
    12601 agagtagtca ggggtaatgg cgatggtttt agtgcctttg tagcgtactt cggtaaagaa
    12661 atgggcgtcc ggcgtacgtg tctgcggtac gttagacccc caggcgatga tgtaactgga
    12721 gttataccag tcggcagatt ccggcacgtc ggtttgttcg ccccaggtca tcggcgaggc
    12781 gggcggcagg tcgcaatacc agtcgtagaa gcttaaacag gtgccaccta atagtgaaag
    12841 ataacgtgtt ccggcggcgt aagaaaccat cgacatcgcc gggatcggcg agaaaccggc
    12901 aacgcgatcc gggccgtaag ttttgatggt ccagacgtta gcagcggcaa tcagctggtt
    12961 tagctcctgc cagttggagc ggataaagcc gccgcgtcca cgtacttgtt tgtagcttag
    13021 gcacttttgc ggatcgttca taatcgatgc ccacgccagt accggatcgc tgtgttgctt
    13081 gagggcttcg cgccacagtt caatcagtcg tttacgaatg agagggtatt tcaggcggtt
    13141 agcgctgtag agataccagg agtaacttgc gccgcgcggg cagccgcgag gttcatgatt
    13201 gggcaggtca gggcgagtgc gcgggtagtc ggtctgttgg atttcccagg tcaccagacc
    13261 atttttaacg tagattttcc agctacagga gcctgtacag ttaacaccgt gggtggaacg
    13321 cacgattttg tcgaactgcc aacgctggcg atagctgtcc tcccagtcgc ggttgctatg
    13381 catcacctgt ccgtgaccat cggcaaaggt ttcgcccttt tgtttgaagt agcgaaagcg
    13441 atccaaaagt ttactcatga cataactcct gttccaggaa tgaattggga tctgatgcct
    13501 tgtcggatgc gacacggttg tagcctgata agacgtgtca ggcatcgatc tcttattttt
    13561 ggctgaactt ccgccgacca taaaccagcc aggtcagcag cacacaaacg atgtagaaga
    13621 ttaaaaacac tttcatcgcg cctaccggag agccggtcat attgagcgac atgccaaacg
    13681 cctgcggaat gaaaaagccg cccactgcgc caatggctga gataaagccc agagccgccg
    13741 ccgtttcggt gacggcttct ttctgagctt gctcatcact accgcctttc atcttcaccc
    13801 gataaatggt tatctggcga aagatgacgg cgatcatctg gaaagtagaa ccacttccca
    13861 gacctgcggt cagaaacaac cccataaata cggcgtaaaa tgcgatgaaa ttaccggagc
    13921 ctgtgcccgg taaggtaagg aacagcaggg cgctgaaaat cgccataaaa atgaagttga
    13981 tcaacgtcac ccgcacgccg ccgaatttat cggaaatagc accaccaacc gaccgcgcga
    14041 tggcaccaat aaatgggcca aagaacgcca ggcgcagaat attcacatca gggaactgag
    14101 ttttcgccag catggcaaaa cccgcagaaa aaccgataaa cgaaccgaag gtggcaaggt
    14161 aaagcaggct cagcagccag agatgcaggc gttgtaacac tggcagctgg tcggcaattg
    14221 acgcgcgtga actggcgata tcattcatcc ctgaccaggc ggcgatcgtg gcaatcgcca
    14281 gtaatggcac ccaaatccat gcggcattcg ccagcgacat caccgaaccg tcggcctgcg
    14341 gtacgccatt gacgccgaga aaggcaaata cgggtacaaa aatgaccagc ggtgcaacca
    14401 gctgcatcac acttacacct aagtttccta acccgccatt aatcccaagt gcgctccctt
    14461 gtttggcttt tgggaagaag aaactgatat tgcccatgct cgaagcaaag ttcgcacctg
    14521 caaaaccgca tagcaaagcg ataacgataa atatcccaaa aggagtattc ggattctgca
    14581 cggcaattcc gagccagacg caaggaataa tcaggattgc agtactaaaa accgtccatc
    14641 ggcgtccacc aaatataggc accataaagg agtaggggac acgcaataat gcgccagaaa
    14701 cggagggtaa tgcggttaat aaaaagagtt gatcggtagt gaaattaaaa ccgattttat
    14761 tgagattaac agtaactgcg ctaaatagca tccagacaca gaaggcaaga agtagacaac
    14821 tgactgatat ccagaggttt cttcgagcaa tatgttttcc tttattttcc cagaaggccg
    14881 gattttctgg tttccagtcg cgcaaaagat aacgactagt tttctcattt tgcagtgcca
    14941 tattgttcct cacatgtaca cattggtaat gaaaaaaaga caaaacagga ggtaaggcgc
    15001 aatagccagt tattagaatt aaggatgaat taggtgaagt gctgattgaa agaatagata
    15061 agaaagcgta accgcggggg cagaatgggg attaaacaga cagatatgtg ttactaaatg
    15121 taactaacaa gctaaccatt aaacggatgt taagctatgt atgcatgtca tgatccaacc
    15181 cttcagatga tgaatccaaa gggtttatat ttttaacaag accagacact tatagaatta
    15241 gtgataattt attcactaaa aaggccatga gctggaggaa aacgttctgt ccagaaagtc
    15301 tatcggttcc tctcttatcg cctcattgta ataatcgtta ttacagtaag tattgtttat
    15361 ttcatctaag gtcatatttt taatttttat attcctct
//
LOCUS       DAOTRK010000115        15048 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.115, whole genome shotgun sequence.
ACCESSION   DAOTRK010000115 DAOTRK010000000
VERSION     DAOTRK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.115"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            102..437
                     /locus_tag="QIE41_003442"
     CDS             102..437
                     /locus_tag="QIE41_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDS3532289.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            597..1040
                     /gene="yjdN"
                     /locus_tag="QIE41_003443"
     CDS             597..1040
                     /gene="yjdN"
                     /locus_tag="QIE41_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDS3532290.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AKNCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1173..1961
                     /gene="phnC"
                     /locus_tag="QIE41_003444"
     CDS             1173..1961
                     /gene="phnC"
                     /locus_tag="QIE41_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDS3532291.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            1986..3002
                     /gene="phnD"
                     /locus_tag="QIE41_003445"
     CDS             1986..3002
                     /gene="phnD"
                     /locus_tag="QIE41_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS3532292.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3063..3842
                     /gene="phnE"
                     /locus_tag="QIE41_003446"
     CDS             3063..3842
                     /gene="phnE"
                     /locus_tag="QIE41_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDS3532293.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3863..4588
                     /gene="phnF"
                     /locus_tag="QIE41_003447"
     CDS             3863..4588
                     /gene="phnF"
                     /locus_tag="QIE41_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDS3532294.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4589..5041
                     /gene="phnG"
                     /locus_tag="QIE41_003448"
     CDS             4589..5041
                     /gene="phnG"
                     /locus_tag="QIE41_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDS3532295.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRATVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5038..5622
                     /gene="phnH"
                     /locus_tag="QIE41_003449"
     CDS             5038..5622
                     /gene="phnH"
                     /locus_tag="QIE41_003449"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDS3532296.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5622..6686
                     /gene="phnI"
                     /locus_tag="QIE41_003450"
     CDS             5622..6686
                     /gene="phnI"
                     /locus_tag="QIE41_003450"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDS3532297.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSAQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLITECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6679..7524
                     /gene="phnJ"
                     /locus_tag="QIE41_003451"
     CDS             6679..7524
                     /gene="phnJ"
                     /locus_tag="QIE41_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDS3532298.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVLDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7521..8279
                     /gene="phnK"
                     /locus_tag="QIE41_003452"
     CDS             7521..8279
                     /gene="phnK"
                     /locus_tag="QIE41_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDS3532299.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPSNRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8390..9070
                     /gene="phnL"
                     /locus_tag="QIE41_003453"
     CDS             8390..9070
                     /gene="phnL"
                     /locus_tag="QIE41_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDS3532300.1"
                     /translation="MINVQNVSKTFILHQQNGVHLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9067..10203
                     /gene="phnM"
                     /locus_tag="QIE41_003454"
     CDS             9067..10203
                     /gene="phnM"
                     /locus_tag="QIE41_003454"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDS3532301.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRRHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLTQAVKLVTKN
                     PAQALNLQDRGVIGEGIRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10203..10760
                     /gene="phnN"
                     /locus_tag="QIE41_003455"
     CDS             10203..10760
                     /gene="phnN"
                     /locus_tag="QIE41_003455"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDS3532302.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGIGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10747..11181
                     /gene="phnO"
                     /locus_tag="QIE41_003456"
     CDS             10747..11181
                     /gene="phnO"
                     /locus_tag="QIE41_003456"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDS3532303.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNIGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11183..11941
                     /gene="phnP"
                     /locus_tag="QIE41_003457"
     CDS             11183..11941
                     /gene="phnP"
                     /locus_tag="QIE41_003457"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDS3532304.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12100..12429)
                     /gene="yjdP"
                     /locus_tag="QIE41_003458"
     CDS             complement(12100..12429)
                     /gene="yjdP"
                     /locus_tag="QIE41_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDS3532305.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVNDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12498..12815)
                     /locus_tag="QIE41_003459"
     CDS             complement(12498..12815)
                     /locus_tag="QIE41_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDS3532306.1"
                     /translation="MQPVFFIKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEGIDKET
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13068..>15048
                     /gene="yjcS"
                     /locus_tag="QIE41_003460"
     CDS             13068..>15048
                     /gene="yjcS"
                     /locus_tag="QIE41_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDS3532307.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTLRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMA
                     DLVKAKKAKIIGNGAILEEIIACLDNFDLWVNIVTP"
BASE COUNT         3385 a         4443 c         4171 g         3049 t
ORIGIN      
        1 tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc aatctgccgt
       61 tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta ccacactgcc
      121 caaaatgcaa ctccgaatac acctacgaag ataacggcat gtacatctgc ccggaatgtg
      181 cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc gttaaagatg
      241 ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat ctgaaggtga
      301 aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc ctggttgaag
      361 gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg aaatctgagt
      421 ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct cgcgccgcat
      481 ccggcaacga tgccgactgc ctgatgcgac gctgaccgcg tcttatcagg cttccctctc
      541 atctgtataa atttcgaact acacttaagt ggcttctctt aactgaggtc accatcatgc
      601 cgttaagtcc ctacctctct tttgccggta actgtgccga cgcaattgct tattatcaac
      661 gtacgttggg cgctgaactg ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc
      721 aggacagcgc caagaactgc ccttccggaa tgcaatttcc cgataccgcc atcgcccacg
      781 ccaatgtgcg cattgccgga agcgatatca tgatgagcga tgctataccg tcaggaaaag
      841 ccagctactc cggctttacg ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct
      901 ggtttgacaa ccttgccgct aacggaaaaa tcgaaatggc ctggcaggaa actttctggg
      961 cgcatggctt cggcaaagtc accgataaat ttggcgtacc gtggatgatt aatgtcgtca
     1021 aacaacaacc aacgcactaa cccgccggga gggccgccct cccgcactgt catcgaaatc
     1081 tcgttaactc ttcatccgtt agtcactttt agttaacaaa atcgtcacaa taatccgcca
     1141 cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt gtcgagaagc
     1201 tcgccaaaac cttcaatcag catcaggcgc tgcacgcggt tgatctgaac attcatcacg
     1261 gtgaaatggt ggctctgctt gggccgtcgg gttccggcaa atccaccctt ttacgtcact
     1321 taagcggttt gattaccggc gataaatctg ccggtagcca tatcgagctg ctgggccgca
     1381 cagtccagcg cgaaggccgc ctggcccgcg atatccgcaa aagccgcgcc aacaccggct
     1441 acatcttcca acaattcaac ctggtgaatc gcctgagcgt actggagaac gtgctgattg
     1501 gcgcgctcgg cagcacgcca ttctggcgca cctgttttag ctacttcact cgcgagcaga
     1561 aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcactttgcc catcagcgcg
     1621 tttccaccct ctccggcgga cagcagcagc gtgtggcgat tgcccgtgcg ctgatgcagc
     1681 aggcgaaggt gattctggcc gatgaaccca tcgcctcgct ggacccggaa tcagcgcgca
     1741 tcgtgatgga caccctgcgc gacatcaacc agaacgacgg catcaccgtg gtcgtcacgc
     1801 tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg cgtcaggggc
     1861 acgtcttcta cgacggcagc agccaacagt ttgataacga acgctttgat catctctatc
     1921 gcagcatcaa ccgcgtcgaa gagaacgcga aagctgcctg acatccccat cattgaggaa
     1981 aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc agcctcagca
     2041 ccctgttaag cccggcgcac gccgaagagc aggaaaaggc gctgaatttc ggcattattt
     2101 caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gttcttacag gatatggaga
     2161 agaagctggg cgtaaaggtc aacgccttct ttgccccgga ctacgcgggc attatccagg
     2221 ggatgcgctt caataaagtg gatatcgcct ggtacggcaa tctgtcggcg atggaagcgg
     2281 tggatcgcgc caacggtcag gtcttcgccc agacggtcgc agcggatgga tcgccgggtt
     2341 actggagcgt gttgatcgtc aacaaagaca gtccgatcaa caacctgaac gatctgctgg
     2401 cgaagcgtaa agatctcacc ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg
     2461 tccccggcta ctacgtcttc gccaaaaaca atatctccgc cagcgacttc aagcgcaccg
     2521 tcaacgccgg gcatgaaacc aacgcgctgg ccgtcgccaa caagcaggtg gatgtggcga
     2581 ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccagagaag ctcaaagaac
     2641 tgaaggtgat ctggaagtcg ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc
     2701 tttccgaaac caccaaagac aagatctacg acttctttat gaattacggc aaaacgccgg
     2761 aagagaaagc ggtgctggaa cgcctgggct gggccccgtt ccgcgcctcc agcgacctgc
     2821 aactggtgcc gattcgccag ctcgcgctgt ttaaagagat gcagggcgtg aaaggcaata
     2881 aagggctgaa tgagcaggac aagctggcaa aaactagcgc cattcaggcg caactggatg
     2941 acctggaccg cctgaacaac gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt
     3001 aatgtgctcg atcggtcccc tctcccacag ggagagggag aaaacattaa acggagcaat
     3061 atatgcaaac catcaccatc gccccaccca agcgcagctg gttctcgctt ctgagctggg
     3121 ccattgttct cgccgtgctg gtcgtctcgt ggcagggcgc ggaaatggcc ccgctcacgc
     3181 tgattaaaga cggcggcaac atggcgacgt tcgctgccga cttcttcccg cccgatttca
     3241 gccagtggca ggattacctc accgaaatgg ccgtcacgct gcaaatcgcc gtctggggca
     3301 ccgcactggc ggtggttctc tccatcccct tcggtctgat gagcgccgaa aacctggtgc
     3361 cgtggtgggt ttaccagccc gtacgccgcc tgatggacgc ctgccgcgcc attaacgaaa
     3421 tggtcttcgc catgctgttc gtggtcgccg tcggccttgg cccgttcgct ggcgtgctgg
     3481 cgctgtttat ccacaccacc ggcgtgctct ccaagctgct ttccgaagcg gtggaagcga
     3541 ttgaacccgg cccggtggaa ggcattcgcg ccaccggtgc caacaagctg gaagagatcc
     3601 tctacggcgt gctgccacag gtgatgccgc tgctgatctc ctactccctc tatcgcttcg
     3661 aatccaacgt ccgttcggcc accgttgttg gcatggtcgg cgctggcggg atcggcgtca
     3721 ccctgtggga agcgattcgc ggtttccagt tccagcaaac ctgcgccctg atggtgctga
     3781 ttatcgtcac ggtcagcctg ctggatttcc tctctcaacg gttgcgtaag cactttatct
     3841 gataagcgag gcattgatat ctatgcactt gtctacacat ccgaccagct acccaacacg
     3901 ctatcaggag atagccgcaa aacttgagca ggagcttcgt caacactacc gctgcggcga
     3961 ctatcttccc gccgagcagc aactggcggc gcgctttgag gtgaaccgcc acaccctgcg
     4021 ccgcgccatc gaccaactgg tggaaaaagg ctgggtacag cgccgtcagg gcgtcggcgt
     4081 gctggtgctg atgcgcccgt tcgattaccc gctcaacgcc caggcgcgtt ttagccagaa
     4141 cctgctggat cagggcagcc atcccaccag cgaaaaactg ctgtctgtgc tgcgcccggc
     4201 ctccggtcac gtcgctgacg cgctggggat aaccgagggc gagaacgtta tccacctgcg
     4261 caccctgcgc cgggtcaacg gcgtcgcgct ctgtttaatc gaccactact ttgcggacct
     4321 caccctctgg ccgacgctgc aacgcttcga cagcggctcg ctgcacgatt ttctgcgcga
     4381 gcaaacgggg attgcgctgc gccgcagcca gacgcggatc agcgcccgcc gcgcccaggc
     4441 caaagagtgc cagcgtcttg aaatcccgaa tatgtcgccg ctgctgtgcg tgcgcaccct
     4501 taaccaccgt gacggtgaaa gcagcccggc ggagtactcc gtcagcctga cgcgcgccga
     4561 catgattgaa ttcactatgg agcactgaat gcacgcagat accgcgactc gccagcactg
     4621 gatgtccgta ctggcacaca gccaaccggc tgaactggca gcccgcctga aggcgctgaa
     4681 catcactgcc gactatgaag taatccgcgc cgctgaaacc ggcctggtgc agattcaggc
     4741 gcggatgggt ggcaccggcg aacgcttttt tgctggcgac gccacgctca cccgcgccac
     4801 cgtacgcctg actgacggca cgctcggcta cagctgggtg ctggggcgcg acaaacagca
     4861 cgccgaacgc tgcgcgctga ttgacgcgct gatgcagcaa acccgccatt ttcaaaactt
     4921 atcagaaacc cttattgccc cgctggacgc tgaccgtatg gcacgcattg ccgcacgcca
     4981 ggccgaagtg aacgccagcc gggtcgactt ctttacgatg gttcgcggag ataacgcatg
     5041 accctggaaa ccgcttttat gcttcctgtg caggatgccc agcacagttt tcgtcgcctg
     5101 ttaaaggcca tgagcgagcc gggcgtgatt gtcgccctgc atcagctcaa acgcggctgg
     5161 cagccgctga atatcgccac caccagcgtg ctgctgacgc tggccgataa cgacacgccg
     5221 gtgtggcttt ctgcgccatt aagtaacgat atcgtcagcc agagcctgcg ttttcatacc
     5281 aacgcgccgc tggtcaatca gccggaactg gcgaccttcg cggtaacgga tgaggcgatt
     5341 tccagcgaac agcttaacgc cctttccagc ggcaccgctg ttgcgccaga agcgggcgcg
     5401 acgctgattt tacaggtcgt cagcctgagc ggcgggcgca tgctgcgcct tactggcgcg
     5461 ggtattgccg aagaacgaat gatcgctccg cagctgccgg agtgcattct gcacgaactt
     5521 accgagcgcc cgcacccgtt cccgctcggc atcgacctga tcctgacctg cggcgagcgc
     5581 ctgctggcta tcccgcgaac cactcatgtg gaggtgtgct gatgtacgtt gccgtgaaag
     5641 ggggcgaaaa ggcgatcgac gccgcccacg ccctgcaaga gagccgacgc cggggcaata
     5701 ccgatttgcc tgaactgagc gtcgcccaga ttgaacagca gcttaatctt gcagtagatc
     5761 gcgtgatgac cgaaggcggc attgccgacc gcgaactggc ggcgctggcg ctgaaacagg
     5821 ccagcggcga taacgtcgaa gcgattttcc tgctacgcgc ctaccgcacc acgctggcga
     5881 agctggcggt aagcgagccg ctcgacacca cggagatgcg tctcgaacgg cgtatttcag
     5941 cggtttataa agatattccc ggcggccagc tgcttggccc aacctacgac tacacccatc
     6001 gcctgctcga tttcaccctg ctggcaaacg gcgaagcgcc gacgctgacc accgccgaca
     6061 gcgcacaaca gccgtcgccg cacgttttca gcctgctggc gcgtcagggg ctggcgaagt
     6121 ttgaagagga tagcggcgca cagccggatg acatcacccg cacgccgccg gtttacccct
     6181 gctcacgttc ttcccgtttg cagcagctca tgcgcggaga cgaaggctat ttgctggcgc
     6241 tggcctactc cacccaacgc ggttacggac gcaatcatcc gttcgcaggc gaaatccgca
     6301 gcggctatat cgacgtgtcg attgtgccgg aagagctggg atttgcggtg aacgtcggcg
     6361 aactgctgat aactgagtgt gaaatggtca acggttttat cgacccgccg gatgagccgc
     6421 cgcacttcac gcgcggctac gggctggtgt tcggcatgag cgagcgcaaa gcgatggcaa
     6481 tggcgctggt tgaccgcgct ctacaagccc cggagtacgg cgagcacgcg acaggcccgg
     6541 cacaggatga agagttcgtg ctggcgcatg ccgacaacgt cgaagccgca ggctttgtct
     6601 cgcacctcaa actcccccac tacgtcgatt tccaggccga actggagcta ctcaaacgtc
     6661 tgcaacagga gcagaaccat ggctaatctg agcggctaca actttgctta cctcgacgag
     6721 cagaccaaac gcatgatccg ccgcgccatc ttaaaagcgg tggcgatccc cggttatcag
     6781 gtgccgtttg gcgggcgcga gatgccgatg ccatacggct ggggaactgg tggtatccag
     6841 cttaccgcca gcgtgattgg cgaaagcgac gtgttgaagg tgcttgacca gggtgcggat
     6901 gacaccacca acgccgtgtc gattcgcaac ttctttaagc gcgtgaccgg ggtaaacacc
     6961 actgaacgta cggacgatgc gacgcttatc cagacgcgtc accgcatccc cgaaacgccg
     7021 ctgaccgaag atcagatcat tatcttccag gtgccaatcc cggaaccgct gcgctttatc
     7081 gagccgcgcg aaacggagac ccgcactatg cacgcgctgg aggagtacgg cgtgatgcag
     7141 gtgaaactgt atgaagatat cgcccgcttc ggtcatatcg ccaccaccta tgcttatccg
     7201 gtgaaggtga acgggcgcta cgtgatggac ccgtcgccga tcccgaaatt cgataacccg
     7261 aaaatggaca tgatgcccgc cctgcagctg ttcggcgcag ggcgcgagaa gcgcatctat
     7321 gcggtgccgc cgtttacccg cgtggaaagc ctcgatttcg acgatcaccc gttcaccgtt
     7381 cagcagtgga atgagccatg cgccatctgc ggatcgaccc acagctatct tgatgaagtg
     7441 gtgctggatg acgccggaaa ccgcatgttt gtctgctccg ataccgatta ttgccgccaa
     7501 cagagcgagg caaaaaacca atgaatcaac cgttactttc ggtcaataac ctgacccacc
     7561 tttacgcgcc gggcaaaggc tttagcgatg tctcttttga tttatggccg ggggaagtgc
     7621 tgggcatcgt cggggaatcc ggctccggga agaccacgct gctgaaatca atttccgcgc
     7681 gtctgacgcc gcagcaaggg gagattcact atgaaaaccg ttccctctat ggcatgagcg
     7741 aggccgaccg ccgtcgcctg ctgcgcaccg aatggggcgt ggtgcatcag catccactcg
     7801 acggcctgcg ccgccaggtg tcggcaggcg gcaatatcgg cgagcggctg atggcgaccg
     7861 gggcgcgtca ttacggcgat attcgcgcca ccgcgcagaa gtggctggag gaggtggaga
     7921 ttccctccaa ccggatcgac gacctgccga ccaccttttc cggcggtatg cagcaacgtt
     7981 tgcagattgc ccgcaacctg gtgacgcatc cgaagctggt gtttatggat gaaccgaccg
     8041 gcgggctgga tgtatcggta caggcgcgcc tgctcgatct gctgcgcggt ctggtggtgg
     8101 agctgaatct ggcggtggtg attgtcaccc acgatctggg cgtcgcccgc ttgctggcgg
     8161 accgtttgct ggtgatgaag caggggcaag tagtggagag tgggttaacc gaccgcgtgc
     8221 tcgacgaccc tcaccatcca tatacccagc tgctggtgtc atcggttttg cagaattgag
     8281 ccagtgccgg atacggcgta aacgccttat ccggccgaca aatgcgctcc ccgtaggtcg
     8341 gataagacgc gtcagcgtcg catccgacac ccgaacaacg aggcgaaaaa tgattaacgt
     8401 acaaaacgtc agtaaaacct tcatcctgca ccagcaaaac ggcgtgcacc tgcccgtcct
     8461 caaccgtgcc tcgctcaccg tcaacgcagg cgaatgcgtg gtgcttcacg gccactccgg
     8521 cagcggaaaa tcaaccctgc tgcgctcgct gtacgccaac tatctgcccg acgaagggca
     8581 aatccagatc aaacacggtg aggaatgggt agacctggtc accgcgccag cccgcaaagt
     8641 ggtggagatc cgcaaaacca ccatcggctg ggtgagccag tttctgcgcg tcatcccgcg
     8701 tatttcggca ctggaagtgg tgatgcagcc tctgctcgat accggcgtac cgcgtgaagc
     8761 ctgcgccgct aaagccgcgc gtctgctcac ccgcctgaac gtgcctgaac gcctgtggca
     8821 cctggcacca tcgaccttct ccggcggcga acagcagcgc gtcaacatcg cccgtggctt
     8881 tatcgtcgac tatccgattc tgctgctcga cgaacctacc gcctcgctgg acgctaaaaa
     8941 cagcgccgcc gtagtggaac tgatccgcga agccaaagcc cgtggcgcgg ccatcgtcgg
     9001 tatcttccat gacgaagccg tacgcaatga cgtcgccgac cgcctgcacc cgatgggagc
     9061 ctcttcatga ttatcaataa cgtcaaactg gtgctggaaa atgaagtggt gcacggttca
     9121 ctggaagtac aggatggcga aatccgcgcc tttgccgaaa gccagagccg ccagccggaa
     9181 gcgatggacg gcgaaggtgg ctggctactg ccggggctga ttgagctgca taccgataat
     9241 ctggataaat tcttcacccc gcgcccgaag gtcgactggc ccgcccactc ggcgatgagc
     9301 agccacgatg cgctgatggt ggcgagcggc atcaccaccg tgctggacgc ggtggcgatt
     9361 ggcgacgtgc gcgacggcgg cgatcggctg gagaatctgg agaagatgat caacgccatt
     9421 gaagagacgc agaaacgcgg cgtcaaccgc gccgagcacc gcctgcacct gcgctgcgaa
     9481 ctgccgcatc acaccacgct gccactgttt gaaaaactgg tgcagcgcga gccggtgacg
     9541 ctggtgtcgc tgatggatca ctcgccggga cagcgccagt tcgccaaccg cgagaagtat
     9601 cgcgaatatt atcagggcaa atactcgctc accgatgcgc agatgcagca gtacgaagaa
     9661 gagcaactgg cgcttgccgc acgctggtca cagcctaatc gcgaatccat cgccgccctg
     9721 tgccgcgcgc gacaaattgc cctcgccagc cacgatgacg ccacgcacgc ccacgttgcc
     9781 gaatcccacc agcttggcag cgtgatcgcc gaatttccca ccacgtttga agcggcggaa
     9841 gcttcgcgca ggcatggtat gaacgtgctg atgggcgcgc cgaatatcgt gcgcggcggt
     9901 tcgcactccg gcaacgtggc ggccagtgaa ctggcgcagc ttggcctgct ggatatcctc
     9961 tcttccgact actaccccgc cagcctgctc gatgcggcat ttcgcgtcgc cgacgacgag
    10021 agcaaccgct ttacgctgac gcaggcggtg aagctggtga ccaaaaatcc ggcccaggcg
    10081 ctcaatcttc aggatcgcgg ggtgattggg gagggcatac gcgccgacct ggtgctggcg
    10141 catcgcaagg gcaaccacat tcatatcgac cacgtctggc gtcagggtaa aagggtgttc
    10201 tgatgacggg aaaactgatt tggttaatgg gggcctctgg ctccggaaaa gacagtctgt
    10261 tgacggaact ccgccagcgg gaacaaactc agctactggt agcgcatcgc tacatcacgc
    10321 gcgccgccag cgccggaagt gaaaaccata tcgccctgag cgagcaggag ttttttaccc
    10381 gcgcggggca gaaccttctg gccttaagct ggcacgccaa cggcctgtat tatggcatcg
    10441 gcgtcgagat tgacctctgg ctgcacgctg gatttgacgt ggtggtcaac ggctcacgcg
    10501 cccatctgcc gcaggcgcgg gcgcgctatc aatcggcgct gctgcccgtt tgtttacagg
    10561 tttcgccgga gatcctgcgc cagcgcctcg aaaaccgtgg tcgtgaaaat gccagtgaaa
    10621 ttaacgcccg actggcacgc gctgcccgct atactcctca ggattgtctt acgctcaata
    10681 atgacggcag cctgcgccag tcggtcgaca agctgctgac gctgattcat cagaaggaga
    10741 aacaccatgc ctgcttgtga gcttcgcccg gctacgcagt acgacaccga cgcagtttac
    10801 gcgctgattt gtgagctaaa acaggcggag tttgaccacc acgcgtttcg cgtggggttt
    10861 aacgccaatc tgcgcgaccc gaacatgcgc taccatttgg cgctgctgga tggcgaagtt
    10921 gtcggcatga tcggcctgca tttgcagttt catctgcatc atgtcaactg gatcggcgaa
    10981 attcaggaac tggtggtgat gccgcaggcg cgcggtctga acatcggcag taaattactg
    11041 gcatgggcag aagaagaagc ccgccaggcc ggggccgaaa tgaccgagct ttccaccaac
    11101 gtgaagcgcc acgacgcgca ccgtttctat ctgcgcgaag gctacgagca gagccatttc
    11161 cgcttcacca aggcgctgta acatgagcct gaccctcaca cttactggca ctggcggcgc
    11221 acagggcgtt cccgcatggg gctgtgagtg cgcggcctgc gccagagcgc ggcgctcacc
    11281 gcagtatcgc cgccagccat gcagcggcgt cgtgaagttt aacgacgcga tcaccctgat
    11341 cgacgccggg ctgcacgatc tcgccgatcg ctggtcgccc ggatcgttcc agcagttttt
    11401 gctgacgcac taccatatgg atcacgtgca ggggctgttt ccgctgcgct ggggcgtggg
    11461 cgatcagatc ccggtctacg gcccgccgga tgaacagggc tgcgacgatc tgtttaaaca
    11521 tccgggcctg cttgatttca gccacacgat ggaaccgttt gtggtgtttg atttacaggg
    11581 gttacaggtc acgcccctgc cgctcaacca ctcaaaactc accttcggct atttgctgga
    11641 aaccgcgcac agccgcgtgg cgtggctgtc tgacaccgca ggcctaccgg aaaaaacgct
    11701 gaaattttta cgcaataatc aaccgcaggt gatggtgatt gattgcagcc atccgccgcg
    11761 cgaggatgcg ccacgtaatc actgcgattt aaacaccgtg ctggcactga atgaggttat
    11821 ccgctcgcca cgggtgattc tgacgcatat cagccaccag tttgatgcgt ggctgatgga
    11881 aaacgcactg ccgtcggggt ttgaggcggg gtttgatggg atggagattg gggtggcgtg
    11941 atgggaggga atgtgcgcgc tggccccctc accctaaccc tctccccaga ggggcgaggg
    12001 gaccgattgt gctcgatatt gaatattgcg ctggttttct ccctcgcccc attggggaga
    12061 gggccggggt gaggggcgat gcctgctcca tacccaacat caacgcccat actcatcttc
    12121 cattctccgc tcttcatctt ctagctgccg acgctcctga tcaagctggc gctggcgatc
    12181 gtccagctgc ctgcggcgat cttcaaactg gcggcggcga tcgtcatatt gtctgcgccg
    12241 atcgtcgttc acttcacgct gccatccgtc gtcgcgcgaa tcttcatagt ctcgcccacg
    12301 gtcagggtta taagcatcgt taatcgcctg ctgaatattg ccaatggtgt cgtcgataat
    12361 atcggcctgg gccggaacgg tggacagcgt gagcagggtg aataaaagaa atagcggaaa
    12421 gcgtttcatt agccaacctc aaaaagaaac tctatccaca ttagtcatta ctcatccatg
    12481 caagtagtgg atgaatctca atttctccgc tgctctattg ccgtaatcgc ctccacgcgt
    12541 tgttgatgac gaccgccttc gtactgcgcg cccagccacg catccacaat catttttgcc
    12601 agttcgaggc caaccactcg tgaaccaaaa gccagtgcaa cttcactggc gtaatgcgga
    12661 taatcagtgc gctctgacga cctggtttct ttatcaatcc cctcactcat tgaccatgaa
    12721 taaaaggctg gcacagcaac gcagaatcct gcggtgccag cgacatgacc gcccattgct
    12781 ccggtacgtt ttttaataaa aaacaccggt tgcattgatt aattgtgctc agtatttgcc
    12841 gaagtacaat atatataata aatatataaa acacctcata ttatttacag agataataaa
    12901 ttcgcgtact tttccctctt cagcacaaat gcctaataaa atggcgcgtt cagctcacac
    12961 ttctacttat taaagtcgca atttcccaat tatgataacg ccagcttcaa atgacgtatt
    13021 ttgcatgggt tacatattta cgacataaat gataaatgga gatttatatg aataactctc
    13081 ggttattccg tttgagcagg attgttattg cgttaactgc cgccagcagc atgatggtaa
    13141 ataccgctaa tgcgaaagag gaagcgaaag ccgccactca atatacccaa caggttaatc
    13201 agaattacgc caaatcatta ccgtttagcg atcgtcaaga ttttgacgat gcccagcgtg
    13261 gatttatcgc cccgctgctg gatgaaggta ttctgcgtga tgcgaacggt aaagtttact
    13321 accgcgcaga cgattacaaa tttgatatta atgccgctgc gccggaaacc gtaaacccca
    13381 gcctgtggcg tcagtcgcaa atcaacggta tttctggcct gttcaaagtc accgataaaa
    13441 tgtatcaggt gcgcggccag gatatctcta acattacgtt cgttgagggc gagaaaggca
    13501 ttattgttat cgacccgctg gtgacgccgc ctgccgcaaa agccgcactt gatctttact
    13561 tccagcatcg tccgcaaaaa ccgattgttg ctgtgattta cacccacagc cacgccgacc
    13621 attatggcgg tgtgaaaggc attatctctg aagcggatgt taaatccggc aaagtgcagg
    13681 tgatcgctcc ggcaggcttt atggacgaag ccatcagcga aaacgtgctg gcgggtaaca
    13741 tcatgagccg ccgtgcgctc tactcttacg gtctgttact gccgcacaac gcgcaaggca
    13801 acgtgggcaa cggcctgggc gtgacgctgg caacgggcga cccgagcatt attgcaccga
    13861 ctaaaactat cgtcagaact ggcgagaaga tgattatcga cggcctggaa tttgacttcc
    13921 tgatgactcc gggtagcgaa gcgccagccg aaatgcactt ctatattccg gctctgaaag
    13981 ccctgtgtac cgccgagaac gccacgcata ccctgcacaa cttctacact ctgcgcggcg
    14041 cgaaaacccg cgacaccagc aagtggaccg agtatctgaa cgaaacgctg gatatgtggg
    14101 gtaatgacgc ggaagtactg tttatgccgc acacctggcc ggtctggggc aataagcata
    14161 tcaatgatta tattggtaaa tatcgcgata ctatcaagta cattcacgac cagaccctgc
    14221 acctggctaa ccagggctac accatgaatg aaatcggcga catgattaag ctgccgcctg
    14281 cgcttgccaa taactgggcc agccgcggct attacggttc tgtcagccac aacgcccgcg
    14341 cggtgtataa cttctatctt ggctattacg acggtaaccc ggctaacctg catccgtatg
    14401 gtcaggtgga gatgggtaaa cgttacgtga aagcgctggg cggttctgcc cgcgtcatca
    14461 acctggcgca agaagcgaac aagcaaggtg attaccgctg gtcggcagaa ctgctgaaac
    14521 aggtgattgc cgccaaccct ggtgaccagg tcgcgaagaa tctgcaagcg aataactttg
    14581 aacagctggg ctatcaggcc gagtccgcca cctggcgcgg tttctacctg accggcgcga
    14641 aagagctgcg cgaaggggtg cataaattca gccacggcac caccggttcc ccggacaccc
    14701 ttcgcggaat gtcggtcgaa atgctgttcg actttatgtc cgttcgcctc gatagtgcga
    14761 aagccgcggg taaaaatatc agcctgaact tcaatatgag caacggcgat aacctcaacc
    14821 tgacgctgaa cgatagcgtg cttaactacc gtaaaacgct gcaacctgaa gccaatgcct
    14881 ctttctacat cagccgtgaa gatcttcacg ccgtgctgac cggacaagtc aaaatggcgg
    14941 atctggtaaa agcgaagaaa gccaaaatta ttggcaatgg cgcgatactg gaagaaatta
    15001 ttgcctgtct ggataatttc gatttgtggg tgaatatcgt aaccccaa
//
LOCUS       DAOTRK010000116        15010 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.116, whole genome shotgun sequence.
ACCESSION   DAOTRK010000116 DAOTRK010000000
VERSION     DAOTRK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.116"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..794)
                     /gene="fkpA"
                     /locus_tag="QIE41_003461"
     CDS             complement(<1..794)
                     /gene="fkpA"
                     /locus_tag="QIE41_003461"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDS3532308.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAA"
     gene            1015..1233
                     /locus_tag="QIE41_003462"
     CDS             1015..1233
                     /locus_tag="QIE41_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDS3532309.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(1283..1873)
                     /gene="slyD"
                     /locus_tag="QIE41_003463"
     CDS             complement(1283..1873)
                     /gene="slyD"
                     /locus_tag="QIE41_003463"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDS3532310.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(1968..2168)
                     /locus_tag="QIE41_003464"
     CDS             complement(1968..2168)
                     /locus_tag="QIE41_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDS3532311.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(2178..3983)
                     /gene="kefB"
                     /locus_tag="QIE41_003465"
     CDS             complement(2178..3983)
                     /gene="kefB"
                     /locus_tag="QIE41_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDS3532312.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGAADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVLSVIVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(3983..4534)
                     /gene="kefG"
                     /locus_tag="QIE41_003466"
     CDS             complement(3983..4534)
                     /gene="kefG"
                     /locus_tag="QIE41_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDS3532313.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            4665..6584
                     /locus_tag="QIE41_003467"
     CDS             4665..6584
                     /locus_tag="QIE41_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3532314.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDHKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKEKENANSAQARKDQKRREAELRAQTQPLRKEI
                     ARLEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLE
                     AQEQLEQMLLEGQSN"
     gene            6584..7606
                     /locus_tag="QIE41_003468"
     CDS             6584..7606
                     /locus_tag="QIE41_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDS3532315.1"
                     /translation="MAQITTTNANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQRESGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            7600..7818
                     /locus_tag="QIE41_003469"
     CDS             7600..7818
                     /locus_tag="QIE41_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDS3532316.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            7872..8741
                     /locus_tag="QIE41_003470"
     CDS             7872..8741
                     /locus_tag="QIE41_003470"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDS3532317.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(8796..9200)
                     /locus_tag="QIE41_003471"
     CDS             complement(8796..9200)
                     /locus_tag="QIE41_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDS3532318.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            9502..10134
                     /gene="crp"
                     /locus_tag="QIE41_003472"
     CDS             9502..10134
                     /gene="crp"
                     /locus_tag="QIE41_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDS3532319.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            10185..12275
                     /locus_tag="QIE41_003473"
     CDS             10185..12275
                     /locus_tag="QIE41_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDS3532320.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12338..13561)
                     /gene="argD/dapC"
                     /locus_tag="QIE41_003474"
     CDS             complement(12338..13561)
                     /gene="argD/dapC"
                     /locus_tag="QIE41_003474"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDS3532321.1"
                     /translation="MATEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGGK"
     gene            complement(13647..14210)
                     /gene="pabA"
                     /locus_tag="QIE41_003475"
     CDS             complement(13647..14210)
                     /gene="pabA"
                     /locus_tag="QIE41_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDS3532322.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPVCFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(14242..14844)
                     /locus_tag="QIE41_003476"
     CDS             complement(14242..14844)
                     /locus_tag="QIE41_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDS3532323.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKEKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(14834..15001)
                     /locus_tag="QIE41_003477"
     CDS             complement(14834..15001)
                     /locus_tag="QIE41_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDS3532324.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
BASE COUNT         3659 a         4205 c         3755 g         3391 t
ORIGIN      
        1 gcggctttcg catcagcttc cggctttgca tcagccttcg gcgctggttt cacatccagc
       61 agctctacgt caaacaccag ggtagaattc ggtgggatcc ccggaacacc cgctttgccg
      121 taagccagtt ctggtggaat aaccagtttg atcttaccgc ctttcttgat gttcttcaga
      181 ccttctgtcc aacccggaat aacaccgtcc agacggaaag agagcggttc accacgggtg
      241 taagagttgt cgaactcttt accgtcgatc agcgtacctt tgtagttcac gacaacagta
      301 tcgctgtctt tcggtgcttc gcctttaccg gcttcgacta cctgataaac cagaccagtt
      361 gaagaggttt tcacaccttt ctctttggca aatttctcgc ggtactcttt acctttcgct
      421 tcgttatctg ccgcgtcttt ttccatcttc gcctgagcag aagacttcac gcgagcttcg
      481 aatgcttgca gtgtctgttc gatctcttgg tcggagagtt tgctcttatc agcaaatgca
      541 tcctgaacac cagcgatcag ctgatcttta tccagtttga tgcccagttt ttcttgttct
      601 ttaagagagt tttccatgta acgacccagc gaggcaccca gtgcataagc tgatttctga
      661 tcgtcatttt tgaacgctgc tttgctgtca gcagttgtag caggttttgc agcttcagca
      721 gcaaaagtga ttggtgcatg cagggcaacg gccattgtgg tcgccagcag cgttacttta
      781 aacagtgatt tcatccatat ctccaggatc ggggcatctc accccagggt taactattat
      841 cagaagggta ctataaagcg ttgtcgaaca aatctacata cagacacgcc ctattatcat
      901 ctattttcag actctttttg tttaaattag tttcgatgac cgcgaaatga gtgctgtctc
      961 gggcagcaaa gttaagtaga atccgcggcg accattcgac aaaagaggtg aatcatgcag
     1021 gatttatcat tggaagcacg cctggcagag ctggagagcc gactggcttt tcaggaaatc
     1081 accattgaag aactgaacgt cacggtgacc gctcatgaaa tggagatggc gaaactgcgc
     1141 gatcatctgc gtctgctgac cgagaagcta aaagccagcc agccgtcgaa catcgcgtcg
     1201 caggctgaag aaacgccacc gccacattat tgaggcgtaa aaaaagcggg gattcccccg
     1261 cttttttgtc actttttcgg tattagtggc aaccgcaacc gccgttacct ttaccgccgc
     1321 agcagccttc gccaccgtgt tcatgaccgt gatcgtggcc atgaccaccg cagcaaccgt
     1381 cgtggtcgtg atcgtggtga tgatcgtgcg caccgtgaac gtggccatga gccagttctt
     1441 cttcagtcgc ttcgcgaatc gccacaactt caacgttgaa tttcaggttc tgaccggcca
     1501 gcatgtggtt accatcaacc acgacgtgat cgtcttcaac cgcagtgatt tcaaccggta
     1561 ccggaccctg gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca
     1621 taaatacgtc tttaggaacg cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt
     1681 tcgcgccaac agcgacatca aatttgtcgc caacttcgtg accttccagc gccgtttcca
     1741 ggccagagat cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact
     1801 catcaaccaa cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt
     1861 ttgctacttt catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa
     1921 ttctgcagcc gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga
     1981 aaaatcccga tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtccgcttct
     2041 cgcatctgat gtccgcactt aacacattca acaatatcaa tattattttc gcgccacatc
     2101 gccattgaat cctgcgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg
     2161 attgccatct ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt
     2221 tgcatttcac gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt
     2281 tgtacggtat cggtatgcat tgggatgagc tctcgcagca tccgcatatc caggcgacga
     2341 aaatgcagtt gcgcccgctg tgcctgatgc ggatgcatgc caagcgtgac cagcgtcttg
     2401 cgccccagct ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt
     2461 aataactcat gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag
     2521 tgctgctggc atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg
     2581 atagactcag cggcctctgc tcccgcagaa cgtaaaagat cgacctgcgt ggcgtcgccg
     2641 taataaactt tgtagccata tttacgcatc aggttaacgg cactgatatc ccgctccagc
     2701 acagtaatgc gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgccca
     2761 aagcccacga caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct
     2821 tccggtccgt taaactggcg ggatagccat ttatccacca gcttcatcag cagcggcgtg
     2881 gtcatcatgg aaagcgtcac cgtcaccagc aacagcgcca tctggtcacc ctggaataag
     2941 cgttgtgaag aagcggtaga aaagagcaca aaggcaaact caccgccctg actcaacacg
     3001 ccagcaaact gcatccgctc tgagctacgc acgccataca gtcgcgccag cagatacagc
     3061 acgagaattt tcaccgccac caacacaata acgcttaaca ccacccacaa cagatgggta
     3121 taaagcacac cgaggttgag cgacattccg accgagataa aaaatagccc gagcagcaac
     3181 cccttaaacg ggtcgatagc tgtttccagt tcgtggcgat attcgctttc tgccagcaac
     3241 acgcccgcaa taaacgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg
     3301 gaacccaaca ccagcagcag cgtcgcggcg gtgaacactt cccgcacacc agaagctgca
     3361 ataaaacgga ataccggacg cagtaaataa cgcccgccaa tcagcatgcc gacaaacgcc
     3421 agcaccttca tgccgatttt catccagtcg aaatgttcgt ctgccgcccc cgccaacaac
     3481 ggcactaacg ccagtgccgg gatcaccgcc agatcctgaa acagcagtac cgagaaacct
     3541 aactggccgg attcgctgcg attcatccct ttctcacgca tcagttgcaa cgccattgcg
     3601 gttgaggaca tcgcaaggcc aataccaccg accaccgccg cctgccaggc gaaatccgtc
     3661 agcatcaata atcccgccag caacgccgcg cttaacagca cctgtgccgc acctacgcca
     3721 aaaatcgaac gccgcaattg ccaaagcttg gagggattca actccaggcc gatgataaac
     3781 atcaggaaca ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaaa
     3841 cccagccccc acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaata
     3901 cccagccgcg atgccagcgg caccgcagcc accgccgcga agagaaacaa cactcctgcg
     3961 agtaaaaaat cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt
     4021 caccgtaggc tctggcgtga ctcgccagct cctgtgcgct ttgccgtctc gcccagtaaa
     4081 taatgatggg actcaaccaa tgcatccggc acatgcccgc cgctagttca aaggggcgca
     4141 gaacatcgct catcgggtag cgattcaagg cgtcataacg gtaagcactt tccggctcgc
     4201 cggtggtaat cacgctacgc cagtactttc ccgccagttg gtttcccccc ggcccgctgg
     4261 caaaaccacg acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg
     4321 tataaagagg atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg
     4381 ggatatcaat aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga
     4441 gctgcgtggc cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg
     4501 catacagcag caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt
     4561 gtatagtcgc tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca
     4621 ttatcataat gataagttaa catagtctga acatacggca ccttatgatt gttttctcct
     4681 cgttacaaat tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc
     4741 ccgggcagaa agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat
     4801 tgctgaaaaa tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac
     4861 tggcgtgggt gaatcaggaa acgccggcgt taccgcaagc ggcgctggaa tatgtcattg
     4921 acggcgaccg tgaatatcgt caactggaag cgcagctaca cgacgccaac gaacgtaacg
     4981 acgggcacgc catcgccact attcatggca agctggatgc tatcgacgca tggagtattc
     5041 gctcccgtgc cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc
     5101 cggtaagtga tttctctggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt
     5161 gccgttcaga cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta
     5221 tctggctgga aaaatggctg aagagctatc agggcacgct gatcctgatc tctcacgacc
     5281 gcgacttcct cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg
     5341 agtacaccgg caactacagt tcgtttgaag tgcagcgcgc cacccgtctg gcgcagcaac
     5401 aagcgatgta cgaaagccag caggaacgcg tggcgcatct gcaaagttat atcgaccgtt
     5461 tccgtgccaa agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta
     5521 tggagctgat tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg
     5581 aaagcctgcc aaatccgtta ctgaagatgg aaaaggtcag cgcaggctat ggcgatcgca
     5641 ttattctcga ctcgattaaa ctgaatctgg tccccggctc gcgcattggt ctgctaggcc
     5701 gcaacggcgc gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca
     5761 gcggtgaaat tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg
     5821 aatacctgcg cgccgacgaa tcgcctattc aacatctggc acgtttagcg ccgcaggaac
     5881 tggagcaaaa actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtgacgg
     5941 aagaaacgcg ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct
     6001 ggcagcgtcc gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc
     6061 gtcaggcact gactgaagca ttaatcgagt tcgaaggcgc gctggttgtc gtctcgcacg
     6121 accgtcattt gctgcgttcc accactgacg atctctacct ggttcacgat cataaagtcg
     6181 aaccgttcga cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg
     6241 aaaaccagac cgacgaagcg ccaaaagaga aagagaacgc gaacagcgcc caggcacgta
     6301 aagatcagaa acgtcgggaa gcggagctgc gtgcgcaaac ccagccactg cgtaaagaga
     6361 ttgcccgtct ggaaaaagag atggagaagc tgaacgcaca gctggcgcag gcggaagaga
     6421 aactcggcga cagcgaactg tacgatcaaa gccgtaaagc ggagttgacc gcctgcctgc
     6481 aacagcaagc cagcgccaaa tccggcctgg aagagtgcga aatggcgtgg ctggaagccc
     6541 aagagcagct tgagcagatg ctgctggaag gccaaagcaa ctgatggcgc agataacgac
     6601 gaccaatgcc aatgaattca gcagcagtgc tgaattcacc cctatgcgcg gctttagcaa
     6661 ttgtcatcta caaaccatgc tgccgcgtct gtttcgtcgc aaggtgaaat tcaccccgca
     6721 ctggcagcga ctggagttgc ccgacggcga ttttgtcgat ctcgcgtgga gtgaagatcc
     6781 tgcacaggcg cggcataaac cgcgtttagt cgtgtttcac gggctcgaag gcagtctcaa
     6841 tagcccttac gcccacggtc tggttgaggc ggcgcaaaag cgtggctggc tgggcgtggt
     6901 gatgcatttt cgcggatgca gcggtgaacc aaaccgtatg caccgcattt accattcggg
     6961 cgaaaccgaa gacgccagtt ggtttttacg ctggctgcaa cgcgagtctg gtcatgcgcc
     7021 aacggctgcc gtcggctatt cgctcggcgg taatatgctg gcctgtttgc tggcaaaaga
     7081 aggcaatgat ctcccgattg atgcggcggt gattgtctct gcgccgttta tgctggaagc
     7141 ctgtagctat catatggaaa agggcttttc ccgcgtctat cagcgttact tgctgaacct
     7201 gttaaaagcc aatgccgcac gcaagctggc tgcctacccc ggaacgctgc cgattaatct
     7261 cgcgcagtta aaatcggtac gtcgcatccg tgaatttgac gatctaatca ccgccagaat
     7321 tcacggctac gctgacgcta tcgactatta tcgtcagtgt agcgccatgc cgatgctcaa
     7381 tcggatagcc aaaccgacgc tgattattca cgccaaagac gatccgttta tggatcatca
     7441 ggtgatcccg aaaccggaaa gtctcccccc gcaggtggag tatcagctga ctgaacatgg
     7501 cggtcatgtt ggctttattg gcggtacatt acttcatccg caaatgtggc tggagtcacg
     7561 cattcctgac tggttaacaa cgtatctgga ggcgaaatca tgttgattcc gtggcaagac
     7621 ctctcccccg aaacgctgga aaatttaatt gaaagctttg tgttacgtga aggcaccgat
     7681 tatggtgaac atgagcgtac acttgaacag aaagtcgccg acgtcaaacg ccagctacag
     7741 tgcggagaag cggtgctggt atggtcggag ctgcacgaaa cggtcaatat catgccgcgc
     7801 agtcagtttc gcgaataacc tgcaaccgtc ggcggacgag ataataatga tagctaagtc
     7861 atggagttac tatgtctgcc aaacatccgg tcattgcggt aacaggatcc agcggcgcgg
     7921 ggaccaccac caccagcctc gcgtttcgta aaatattcgc gcagttaaat ctgcatgcag
     7981 ctgaggtgga aggcgacagt tttcaccgtt acacccgccc ggaaatggac atggcgatcc
     8041 gcaaagcgcg cgacgccggg cggcatatca gctacttcgg ccccgaagcg aacgactttg
     8101 gcctgctgga acaaaccttc attgaatatg gtcagagcgg caaagggaaa tctcgcaaat
     8161 atctgcatac ctacgatgaa gccgtaccgt ggaatcaggt accggggaca ttcaccccct
     8221 ggcaaccttt accggaaccc acggatgtac tgttttatga aggtttacac ggcggcgtag
     8281 tcacgccaca gcataacgtt gcgcagcatg tggatttact ggttggcgtg gtgcctatcg
     8341 ttaaccttga gtggattcaa aaactgatcc gcgacaccag cgagcgcggg cactcacgag
     8401 aagcagtgat ggactcggta gtgcgttcaa tggaagacta tatcaactac atcacaccgc
     8461 agttttcccg cacccatctt aacttccagc gcgttcccac cgtcgacacc tcaaacccgt
     8521 tcgcggcaaa aggtatcccg tcgctggatg aaagctttgt ggtgatccat tttcgtaatc
     8581 tggaagggat cgattttccc tggctgctgg cgatgttgca aggctcattc atttcccaca
     8641 tcaatacttt agtggtgcct ggcggcaaaa tgggtctggc aatggaatta attatgctgc
     8701 cgctggtgca acgattgatg gaagggaaga aaatcgagta actctgctat tacgccggat
     8761 aaaatactat ccggcttcac aacgggatag ttaaatcacg cggcaaccac ttcatacgag
     8821 tgagtaatat tcaccgcttt ttccagcatc aacgccactg aacaatattt ctcggcagag
     8881 agatctaccg cacgcgcaac cgctgcgtct ttcaggtcgc gaccggtgac gataaaatgc
     8941 agattaatgt gcgtaaacag gcgtggtgcc tcttcgcggc gttcagaggt caattttact
     9001 tcacaatcga ccacatcctg acgccctttt tgcagaatag aaaccacatc gatggcactg
     9061 caaccacccg ccgccatcaa caccatttcc atcggacttg gtgctttatc gcctgagttg
     9121 ccgtccatta aaatctggtg accagaggcg gattcgccca gaaaagttaa cccttcgacc
     9181 cacttcactc gcgcttgcat ttttgctact ccactgcgtc aattttcctg acagagtacg
     9241 cgtactaacc aaatcgcgca acggaaggcg acctgggtca tgctgaagcg agacaccagg
     9301 agacacaaag cgaaagctat gctaaaacag tcaggatgct acagtaatac attgatgtac
     9361 tgcatgtatg caaaggacgt cacattaccg tgcagtacag ttgatagccc cttcccaggt
     9421 agcgggaagc atatttcggc aatccagaga cagcggcgtt atctggctct ggagaaagct
     9481 tataacagag gataaccgcg catggtgctt ggcaaaccgc aaacagaccc gactctcgaa
     9541 tggttcttgt ctcattgcca cattcataag tacccatcca agagcacgct tattcaccag
     9601 ggtgaaaaag cggaaacgct gtactacatc gttaaaggct ctgtggcagt gctgatcaaa
     9661 gacgaagagg gtaaagaaat gatcctctcc tatctgaatc agggtgattt tattggcgaa
     9721 ctgggcctgt ttgaagaggg ccaggaacgt agcgcatggg tacgtgcgaa aaccgcctgt
     9781 gaagtggctg aaatttcgta caaaaaattt cgccaattga ttcaggtaaa cccggacatt
     9841 ctgatgcgtc tgtctgcaca gatggcgcgt cgtctgcaag tcacttcaga gaaagtgggc
     9901 aacctggcgt tcctcgatgt gacgggccgc attgcacaga ctctgctgaa cctggcaaaa
     9961 caaccggatg ctatgactca cccggacggt atgcaaatca aaattacccg tcaggaaatt
    10021 ggtcagattg tcggctgttc tcgtgaaacc gtgggacgca ttctgaagat gctggaagat
    10081 cagaacctga tctccgcaca cggtaaaacc atcgtcgttt acggcactcg ttaatcccgt
    10141 cggagtggcg cgttacctgg tagcgcgcca ttttgtttcc cccgatgtgg cgcaggctga
    10201 tttatcaccc cgatatcaac tatgcacttc gacaaacgct ggtgctatgt ttgcccgtgg
    10261 ccgttgggtt aatgcttggc gaattacgat tcggtctgct cttctccctc gttcctgctt
    10321 gttgcaatat tgcgggcctt gatacgcctc ataaacgttt tttcaaacgc ttaatcattg
    10381 gtgcgtcgct gtttgccacc tgtagcttgc tgacacagct actactggca aaagatgttc
    10441 ctctgccctt tttgctgacc ggattaacgc tggtacttgg cgtcactgct gagctggggc
    10501 cattgcacgc aaaattgctt cccgcatcgc tgctcgccgc catttttacc ctcagtctgg
    10561 cgggatacat gccggtctgg gaaccgttgc tcatctatgc gttgggcact ctctggtacg
    10621 gattgtttaa ctggttttgg ttctggatct ggcgcgaaca accgctgcgc gagtcactaa
    10681 gtctgctgta ccgtgaactg gcagattatt gtgaagccaa atacagcctg cttacccagc
    10741 acaccgaccc tgaaaaagcg ctgccgccgc tgctggtgcg ccagcaaaaa gcggtcgatc
    10801 taattaccca gtgctatcag caaatgcata tgctttccgc gcaaaataat accgattaca
    10861 agcggatgct gcgtattttc caggaggcgc tggatttgca ggaacatatt tcggtcagtt
    10921 tgcatcagcc ggaagaggtg caaaagctgg tcgagcgtag ccatgcggag gaagttattc
    10981 gctggaatgc gcaaaccgtc gccgctcgcc tgcgcgtgct ggctgatgac attctttacc
    11041 atcgcctgcc gacgcgtttt acgatggaaa agcaaattgg cgcactggaa aaaatcgccc
    11101 gccagcatcc ggataatccg gttgggcaat tctgctactg gcatttcagc cgcatcgccc
    11161 gcgtgctgcg cacccaaaaa ccgctctatg cgcgtgactt actggccgat aaacagcggc
    11221 gaatgccatt acttccggcg ctgaaaagtt atctgtcact aaagtctccg gcgctacgca
    11281 atgccggacg actcagtgtg atgttaagcg ttgccagcct gatgggcacc gcgctgcatc
    11341 tgccgaagtc gtactggatc ctgatgacgg tattgctggt gacgcaaaat ggctatggcg
    11401 caacccgtct gaggattgtg aatcgctccg tgggaaccgt ggttgggtta atcattgcgg
    11461 gcgtggcgct gcactttaaa attcccgaag gttacaccct gaccttgatg ctgattacca
    11521 ccctcgccag ctacctgata ttgcgcaaaa actacggctg ggcgacggtc ggttttacca
    11581 ttaccgcagt gtataccctg caactgttgt ggctgaacgg tgagcagtac atccttccac
    11641 gtcttatcga taccattatt ggttgtttaa ttgctttcgg cggtactgtc tggctgtggc
    11701 cgcagtggca gagcgggtta ttgcgtaaga acgcccatga cgctttagaa gcctatcagg
    11761 aagcgattcg cttgattctg agcgaggacc cgcaacctac gccactggcc tggcagcgaa
    11821 tgcgggtaaa tcaggcgcat aacactctgt ataactcatt gaatcaggcg atgcaggaac
    11881 cggcgtttaa cagccattat ctggcagata tgaaactgtg ggtaacgcac agccagttta
    11941 ttgttgagca tattaatgcc atgaccacgc tggcgcggga acaccgggca ttgccacctg
    12001 aactggcgca agaatattta cagtcttgtg aaatcgccat tcagcgttgt cagcagcgac
    12061 tggagtatga cgaaccgggt agttctggcg atgccaatat catggatgcg ccggagatgc
    12121 agccgcacga aggcgcggca ggtacgctgg agcagcattt acagcgggtt attggtcatc
    12181 tgaacaccat gcacaccatt tcgtcgatgg catggcgtca gcgaccgcat cacgggattt
    12241 ggctgagtcg caagttgcgg gattcgaagg cgtaatgcag gctaaaccat cggatacagc
    12301 gttcacgccg catccgacat ctttggcctg atgcgtctta tttaccccca atcaccttcg
    12361 ccaccgcgtg ggcgaaacga tgcatccctt catcgatatc cgcatcttcc accaccagcg
    12421 acggcgcaaa acgcatcaca tcaggcccgg cattcagcac cattacgcca gcctctgcgc
    12481 ccgcatacag gaaatcacgc gcctgacctt tgtactgtgg tttcagctcc gcgccaatta
    12541 acagccccat accgcgaata tcgctaaaca catcgtactg ctgatcgatc ttctgcaaat
    12601 ggtcaacaaa acgctggcgt ttcgcctgaa tgccttccag cacttcaggg gtattgatga
    12661 tatcaaacgc cgcgcccgct actgcacagg ccagaggatt accgccgtag gtggaaccgt
    12721 gagaaccagg atgaaacgca gaagcaattt ccgccgtggt cagcatggcg ctaatcggga
    12781 agccgccgcc taacgctttc gcagaggtca gaatatccgg cgtcacaccg tagtgcatgt
    12841 aagcaaacaa atcgccagtc cgccccatcc cgcactgcac ttcatcaaac accaataatg
    12901 cctgatgttg atcgcacagc tcgcgcaagc cctgcaaaaa ctctggcgtc gcagccgtca
    12961 cgccgccctc gccctggatc ggctcaacca ccaccgcaca ggtgtgatca tccatcaccg
    13021 ctttcactgc gtggagatcg ttaaagggaa cgtggatgat gtctgccggt ttcggcccaa
    13081 agccgtcgga atattttggc tgcccaccca ccgaaacggt aaacagcgag cgaccatgaa
    13141 aagcgttatg gaaggcaata attttggttt tgaacgggct atgacgcaca caggcgtaat
    13201 ggcgtgccag tttaaaggcg gtttcgttag cttccgtgcc ggagttcata aacaccacgc
    13261 gttcggcaaa cgttgcctca atcagtttac gaccaagacg cagcgccggt tcattggtga
    13321 aaacgttact gatatgccac agagtttcgc cctgggtttt taacgcgttc accagcgcag
    13381 gatggcaatg gcccaacgcc gtaactgcaa tgccacccgc gaaatcgaca tactccttgc
    13441 cttgctgatc ccagattcgg ctcccctgac cttttaccgg aataaactct gccggagcat
    13501 aaatcggcag gatcacttca tcgaaagtcg cgcgtgtaat tgctgtttgt tcagttgcca
    13561 tctcatgatc accctgttac acataaataa atgtgaaatt ataatcacaa aatatgcata
    13621 aaaaatcact taatagcaat cagaaatcag cgatgcagga aattagccag cagttgatgt
    13681 ccttgttcgc tgagaatact ttctggatgg aactgcacgc cttccagatc ccactggcga
    13741 tggcgaatac ccataatctc gcgggtttcg ctccaggccg tcacttcaaa gcacacaggt
    13801 aatgagtcag gttccaccac cagcgaatgg tagcgtgtca cggtaagtgg atttgccagc
    13861 cccttaaata cgcccacacc attatgtgta atcggcgagg ttttgccgtg catgaccttt
    13921 gcggcgcgca caactttgcc gccaaatgcc tgcgccattg cctggtgacc gaggcaaacg
    13981 ccaagaatcg gcaagcgccc ggcatagtgg cgaataacgt caagggagat cccggcttca
    14041 tctggcgtac agggtccggg tgagatgaca attttttgtg gtttaagggc gtcgatatcc
    14101 gccagcgtca acgcatcgtt gcgcttaacc agcacatccg cccccagttc acaaaagtac
    14161 tggtagaggt tccaggtaaa agaatcgtag ttatctataa gcaggatcat ggcggctccg
    14221 gtacgaaaga accgcgctat tttactcaga ttccccggct tcgcttacca ctttgctaaa
    14281 tatcatctgc agtgcggtga gatcccccat tgccccactc tgatttgcct gattccaggc
    14341 ctctttttcg ataccctgcc agctcagttg gtagcccgca tgaatcgcca gttgctcgaa
    14401 gaagatccgc tgtgccagtc cgcttcccac acggaaggga tgcagcacgt tgatttcaca
    14461 atagtaatgt gccagccgct cgacgaactt ctctttctcc aggccaacca gataaccttc
    14521 ttcctccaga tcctgcatca gggcattgcc ctctttttcg atataggcaa agtggcagaa
    14581 cggcgtatca ccctgataaa tatcaacttc acgcaattgc cccgcccagt cgaaaatatc
    14641 ctgatacagc tggcgatgga tagtgcgcaa atgcggtaaa ccgcgcacca gcggaccaag
    14701 ctcaatggtc gcagcacgca gcgccgtcat ttcgtaagcg gcctgttcca gccgctgctg
    14761 ctggcggatg ttcagccggt tacgcatgat atcaaggcca ggataaagat acggatcgcg
    14821 cccttcgccg aatttatcgc tcatagtgcc tcctcagctc ttcaaggcgc gctaaagcct
    14881 ctgcggcagt aagagtgact aaaggcatct cgacgccttc aagacggcga ctggcctgga
    14941 aattacgatt acgctgaagc tcccagagac gggacttttg cttatcggtg agtttcttca
    15001 cttgatgcct
//
LOCUS       DAOTRK010000117        14809 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.117, whole genome shotgun sequence.
ACCESSION   DAOTRK010000117 DAOTRK010000000
VERSION     DAOTRK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.117"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            81..1214
                     /gene="hcp"
                     /locus_tag="QIE41_003478"
     CDS             81..1214
                     /gene="hcp"
                     /locus_tag="QIE41_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDS3532325.1"
                     /translation="MSDIIYLNITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSYLAFPDNYNRLFI
                     PRPKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESLEHFGARGAVSGRPFNPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPKGLDGYELWNNVHTATLEDFKIKDTDLFHPDATK"
     gene            1211..1468
                     /locus_tag="QIE41_003479"
     CDS             1211..1468
                     /locus_tag="QIE41_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDS3532326.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSRPRYRVSQYGLNLIKEQQDNLF"
     gene            complement(1957..4218)
                     /gene="metE"
                     /locus_tag="QIE41_003480"
     CDS             complement(1957..4218)
                     /gene="metE"
                     /locus_tag="QIE41_003480"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDS3532327.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPIYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRGDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4336..5289
                     /gene="metR"
                     /locus_tag="QIE41_003481"
     CDS             4336..5289
                     /gene="metR"
                     /locus_tag="QIE41_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDS3532328.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5177..6076)
                     /gene="bioP"
                     /locus_tag="QIE41_003482"
     CDS             complement(5177..6076)
                     /gene="bioP"
                     /locus_tag="QIE41_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDS3532329.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWIGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6152..6952)
                     /gene="yigL"
                     /locus_tag="QIE41_003483"
     CDS             complement(6152..6952)
                     /gene="yigL"
                     /locus_tag="QIE41_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDS3532330.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(6960..7982)
                     /gene="pldB"
                     /locus_tag="QIE41_003484"
     CDS             complement(6960..7982)
                     /gene="pldB"
                     /locus_tag="QIE41_003484"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDS3532331.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8093..8713
                     /gene="rhtB"
                     /locus_tag="QIE41_003485"
     CDS             8093..8713
                     /gene="rhtB"
                     /locus_tag="QIE41_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDS3532332.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8775..9395)
                     /gene="rhtC"
                     /locus_tag="QIE41_003486"
     CDS             complement(8775..9395)
                     /gene="rhtC"
                     /locus_tag="QIE41_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDS3532333.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9459..11288)
                     /gene="recQ"
                     /locus_tag="QIE41_003487"
     CDS             complement(9459..11288)
                     /gene="recQ"
                     /locus_tag="QIE41_003487"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDS3532334.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGIGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11421..12290)
                     /gene="pldA"
                     /locus_tag="QIE41_003488"
     CDS             complement(11421..12290)
                     /gene="pldA"
                     /locus_tag="QIE41_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDS3532335.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12455..12922
                     /locus_tag="QIE41_003489"
     CDS             12455..12922
                     /locus_tag="QIE41_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDS3532336.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12974..13864
                     /gene="rarD"
                     /locus_tag="QIE41_003490"
     CDS             12974..13864
                     /gene="rarD"
                     /locus_tag="QIE41_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDS3532337.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPISLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            14175..>14809
                     /locus_tag="QIE41_003491"
     CDS             14175..>14809
                     /locus_tag="QIE41_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDS3532338.1"
                     /translation="MNNILGSSINMPCTLFETITLFDDFSADDMQYGDMEEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFS
                     FFGQYKTLIQDLIDHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDIS
                     STPTTGYRPLLLQKIKIELLSSRLNKFNDFKDNFNGLGISIHDISAQKISL"
BASE COUNT         3804 a         3767 c         3593 g         3645 t
ORIGIN      
        1 cccttttagg cgtaaataaa ggtaaaaaat aacaatacta tttttctttc acaaaagaat
       61 taacaaaagg tttttcaagg atgagtgata ttatttacct gaacataacc ggagaacaac
      121 aaggatgtat ttcctctcgt tgtggaacga gcgcatcaat aggtaatcgc tggcagattg
      181 gccatgaaga tgaaatcttc gcgttctcgc tctctaatag tattacgaat acaggcaaag
      241 ggtcacaatt gcatggcctt agtttctgta aattgattga caaaagctcc ccgttactta
      301 ttaacgctat taacaataac gagcagttgt tcatggagtt tgacttctat cggatcaata
      361 gatttggtcg atgggagaaa tattacaaca ttcaacttcg cggcgcgctc ttatcagcaa
      421 tcaatcacct gtttactgag aacaatctgg atacagaaac catcactgtc agttatgaat
      481 atatactctc caggcatctg attgctaata ctgaatttag ttatttggca tttcccgata
      541 actacaaccg tctgtttata ccacggccaa aaactaaaga tgataatggc ctcaaaacac
      601 tcaatagtaa agcggttggc agactgcttg ctgctggcgg catatacaat gggaatatcg
      661 aaggattcag ggaaacagca aacaagttgg gaggagatgc acctgctggt tacgatcaag
      721 ttatggataa caaagggtta ctcatcgctg gtgcttcaat agttgctgga gttgttctgg
      781 gaagaatgag attccctgat ttggaatcac ttgagcattt cggagccaga ggagcagtat
      841 caggaagacc atttaatccc gaccttgccg gtggtccaat tgaaaacctg acgatagatg
      901 gcgtaacaat caacagggaa ggtatcgcta ttgttgaaaa gcatatctcc agatttgatc
      961 atgatccagc taatgatgta atgatttcaa ggctaaagaa aatagcaaat aaagaattgc
     1021 ttcctgaaaa atacgatctg aactactata ctcatgaatg tcgcgagtat cagcgttatt
     1081 gtaatttagg atgggaaaca ggtgaaccga aaggtttaga tggatatgaa ttatggaata
     1141 atgtacatac tgcaactcta gaagacttta aaattaaaga tactgactta tttcatccgg
     1201 atgcaacaaa atgacgatta cgatagttga atttaacgtg ctgaaagtta tggctgagaa
     1261 agatattgac tggagctgga tggtgctgga tcgtacttta gctatacgaa atattccagg
     1321 atttggcaac gtagcgaata tcgtaacaaa gctggttaat catgggttgg ttgatatcgt
     1381 aaatggtgag ggcaactcta gaccacgcta cagagtttct caatatggat tgaatcttat
     1441 caaagaacaa caagacaacc tcttctgatc cagccattac aggagcatga tttacattgt
     1501 gatctttcaa taagatactc atcaattttg gtacagaata tcgtgatcac taaaaatgaa
     1561 cttaacgtgc tgaacgttat gactgagaaa gatataaact ggaactggat gaatctcgat
     1621 cgcacactat ccataaggca aattccagga ttcgggaatg ttgttaacat tgttaacaaa
     1681 ttagctaacg aaggtttggt tattattgag gatagcggga ataagtctat gcctcactac
     1741 cacgttagtg aaaagggata caatctcgtg caacgtgaga ataaataacg tagcgttgat
     1801 tactgaacgg aatcactcaa aactgtgttt tgcgaaaaaa acgattcggt tatttctgct
     1861 cctgaagatc agcttcaatc tggcactcat gcaaaatggc ttccagtgct ggaagccatt
     1921 atcgtactgc aatttacagt gactcgttaa tattcgtcat ccccgacgca aattctgcgc
     1981 cgcctgcacc atgttcgcca gtgccgcgcg ggtttctggc cagccgcgcg ttttcaggcc
     2041 acagtccggg ttgacccaca gacgctctgc cggaatgcgt tttgccgctt tcttcagcaa
     2101 ggcttcaatc cattctacgc tcggtacgtt tggcgagtga atgtcataga cgccaggacc
     2161 gatttcattt ggataatcaa actcttcaaa cgactccagc aactccatgt cggaacgcga
     2221 ggtttcgatg gtgatgacgt ctgcgtccag cgccgcaatc gaatccatga tgtcgttgaa
     2281 ctcgcagtaa cacatgtgag tgtggatttg tgtgtcatct ttcgccacgg cggcgttgat
     2341 acggaaagcc tccacgcccc actggagata ggcatcccag tcgccgcgac gcagcggtaa
     2401 accttcgcgc agtgccggtt catcaatctg gatgatgcca attccagcgg cttccaggtc
     2461 cgccacttca tcacgcagcg ccagcgcaat ttgtttggcg atggtttcac ggctgacatc
     2521 ttcacgcggg aacgaccagc agagaatagt caccgggccg gtcaacatcc ctttcaccgg
     2581 tttatcagtc agggattgcg catacttcgc ccactcaacg gtaatcggtg ccgggcggct
     2641 aacgtcacca ataacaatcg gtggcttcac gcagcgtgaa ccgtagctct gtacccaacc
     2701 gttttgcgta aagacaaaac cgtcgaggtg ctcgccaaag tattccacca tgtcattacg
     2761 ctcagcctcg ccatgtacca gcacatccag tcccaaacgt tcctgctcaa caatggcctg
     2821 cttgatatgt tccgcaatgc ccgtgcggta gttattggcg tcgagattac cctttttaaa
     2881 atccagacgc agggtacgaa tctccgtggt ttgcgggaag gaaccaatcg tggtggtcgg
     2941 ccacgcgggc agtttaaaac gcgcacgctg ggcttcagca cgcacttcat agacattcgc
     3001 acgctgactg tcctgggcgg tgatcgccgc cagacgcttt tctactgccg gattatgaac
     3061 acgagtagag tgacgacgcg cctgaatcgg agcgctccac tctgccagag cagccgtgtc
     3121 accactgttc agcgcatcgc gcagcagtgc cagttcatga catttttgca gggcgaaggc
     3181 aaaccagctt ttcacttctg catcaagacg cgtttccacg ctcaggtcga tggggctgtg
     3241 cagcagtgag caggaagatg ccacccacaa atcgcgtttg ccgacaatgt ccttaatttg
     3301 cgcatacttc tcggtaagat cggcgcgcca gacgttacga ccattgatca gacccgcaga
     3361 cagcagccag tcagaaggca gacgcttgtg cagttcagca acgtcatctt taccatgtac
     3421 aagatcgaca tgcagaccct gaacaggcag cgcagtaatc gtgtcgagat ttggcgttac
     3481 gccttcaaaa taggtggtca gcagcagttt cacctgtccc tggagcgcgt cgtaagcggg
     3541 tttgtatgcg tccagccacg cctgcggcag ttccagtacc agcgcgggtt catcaatctg
     3601 tacccactca atgccgcgtt ttgccagttc tgccagcact tgctgataaa tcggaagaat
     3661 gtcgttcagc aggctcaggc gatcaaactg ttcacctttc actttaccca gccacaggta
     3721 ggtaaccggc cccagcagca caggtttcac cttgtggccc agcgccagcg cctcgtccac
     3781 ttcgtccagc agctgcgtcc aggtcagttt gaactgttgg cctttaacga attccggcac
     3841 catgtagtga tagttggtat taaaccattt ggtcatttcc gctgccgccg caggttcgcc
     3901 agtcggcgca cgtccacgac caatacggaa cagggtgtcg atatctaccg aaccatcttt
     3961 gttctgatga cgcgccggaa cgttacccag cagcagactg gtggtcagta catgatcgta
     4021 ccaggcaaaa tcgcccaccg gcagcaggtc gatacccgct tgcttttgtt gatcccagtg
     4081 acgagcacgc aattcacgcc ctaccgccag cagttcttca cgcgtggagt tccccgccca
     4141 ataactctct tgcgcttttt tcagctcgcg acgcaggcca acgcgaggga aaccgagggt
     4201 gtgattaaga attgtcatta tatgcccctt atggaatttt tagtatttag ccgtccagat
     4261 gtttacacat ccataattgg cggttactgt atattcctca agcgcaaatt attcatgccg
     4321 aagtgaagga ctttcatgat cgaagtaaaa catctgaaaa cgctacaagc gttgcggaac
     4381 tgcggctcgc tcgcagccgc tgcggcgacg ttgcatcaga cgcaatccgc cctgtctcac
     4441 cagtttagcg atctggaaca acgccttggc ttccggctat ttgtgcgtaa gagccagccg
     4501 cttcgcttta caccgcaggg agaaatcctg ttgcaactgg caaaccaggt gctgccacaa
     4561 attagccagg cactgcaagc ctgcaatgaa ccgcagcaga cgcgtctgcg catcgccatt
     4621 gagtgccata gctgtattca gtggctgacg cccgcgttag aaaatttcca taagaactgg
     4681 ccgcaggtcg agatggactt taaatcgggc gtgacgtttg acccgcagcc tgcgttgcaa
     4741 cagggcgagc tggatctggt gatgacgtca gatattctgc cgcgcagtgg cctgcattat
     4801 tcgccgatgt tcgattatga agtgcgtctg gtgttggcac ctgaccatcc gctggcggca
     4861 aaaacgcgta ttacaccgga agatctcgcc agcgagacgc tgttgattta tccggtgcag
     4921 cgtagccgac tggatgtctg gcggcatttt cttcagccgg caggcgtcag cccgtcactg
     4981 aaaagcgtcg ataacacctt attgttgatt cagatggttg ccgcgcggat gggtattgcc
     5041 gccctgccgc attgggtagt agagagtttt gagcgccagg gtctggtggt gaccaaaacc
     5101 ctgggcgaag gcttgtggag ccgactgtac gccgccgtgc gcgatggcga gcagcgtcag
     5161 ccgattactg aagcgtttat tcgctcagcg cgcaatcacg cctgcgatca tctgccgttt
     5221 gtgaagagcg cggagcgacc cacttacgat gcacccacag tgaggccagg atcaccagcg
     5281 cgcctgtaat gaacgttggc cagtgcggct gttggtgcca gatagccagg tttaccagca
     5341 gccctgccgg aacgtgcata ttattcataa tacccagcgt tccggcgtct acttgggttg
     5401 cgccgtagtt ccacataaag tagccaatcc cggaagccac cacgccaaga aacaccagaa
     5461 tgccccattg caacgtggtt tgtggcattt tctgcgcatt tcccagcaag aaccatgcaa
     5521 tcaccgccac caggaacgcg ccaagataga accacgcaaa ggcgttatgc tgcggcatcg
     5581 ggcgagtttc catcagacgt ttgtaaccca ccatgccgat ggcaaaagtg atattggaga
     5641 gctgcaccag cagcaggcca atccagaagt ggtcggtgac ctgatcatag cgaataatcc
     5701 cggcaccaat caccgccaac aaggcgctaa aagcatagcc ccagcgcaga cggcgcttac
     5761 tcatgatgtc ataaatcaac gtaatgtaga gcggcgtcag tacggtgaac agcaacaatt
     5821 cggagaccgt caggtagaga taggcgcgaa aactcagcat atacatcacg ccgagctgca
     5881 tcgcgcccac cagcatatac aggccgaccg ttttcaggct attgccacgg gtacgcagaa
     5941 acggcagaaa aacgagtgcc gccaggccaa cgcgcaccag caccgcaaaa tagctatcga
     6001 cgtgccccgc aaggtactcg ccataaaagc taaaggagaa ggcccacaga atcgtggtga
     6061 tgataagtag cgccacaatg gaaaactctt tgattaacga gtatccattg tagcgaagta
     6121 tcaggttgac aactggccaa ataaagaacg attacgataa atagagctta cgcagataat
     6181 gcggcaccgc gtcgtcggca ttagtaccaa tcacttccag ctccggatgc agatctttca
     6241 gacgctggtg cgcactgccc ataatgcagc ctttccccgc catcgacagc atttcggcgt
     6301 cgttcatccc gtcaccaaag gcaatacaat ccttcaggct gtagcccagt ttcttcgcca
     6361 ccgcttccag cgcatgacct ttcgacacgc cgcccgccat cacttccaga caggttaagg
     6421 tagagaaact aacgttgacg cgatcgcccc agcgagcgtt gatcgcctgc tccagcggca
     6481 gaagttgttc atgggaatcg caggtgaaga acactttgct gacgccttcc ggctccagta
     6541 atcccggctc atacagcgca tatttgaaca ccgcttcttt aaaaaagcgc atctcttccg
     6601 ggcgatggcg attcataaac cattcgtcgt cgcgataaac gttagtaatg atgtccggat
     6661 tgtcgttgac tacgccaaac agatcgctgg caatgtcgcg atccaggtta tgagcaaaaa
     6721 tcagattgcc atccagatcg tgaacgcgcg cgccattgga ggtaatcatg taagacttaa
     6781 tctccagatt atcgcgaatt tgccccacat caacgtggtg acgaccggtc gcaaacacaa
     6841 agttgatgcc gcgcgcggtg agcagcttca gagtttcttt ggcgtaaggg gataacgtat
     6901 ggtcgggaga aagtaacgtg ccatctaaat cagacgcaac aacctggtac ataagaaatt
     6961 taaacctctg tagagcggtt tccgctgggt gagttatgcc tgttgaaaaa atcaacgatg
     7021 gcgtggagcg cgactgagcg cattgcgtcc ttttcaaaaa ggatctcatg gtaagcacct
     7081 ttaattacca acggccgtcc tccttcgaca ggatggcccg cggcggtgcg gagttcacaa
     7141 aaacggtcgt gcatgcggtt atccaccacg cgttcctctt cagcttgtaa aagaagcgtt
     7201 ggcgtggcgt catcacccgc accggctaac acctgttcgc cagccagaat actttcgcgt
     7261 acccaatggt aggttggccc accgacgcga atcgttgggt catcagcata gaagcgtaag
     7321 ttacgtcgat atcgctgtct gctgtgggtc agtacgttga tagcaaacgg caacgcgcgc
     7381 cagcgcccgg tgcctattgc atagccatca cggaaacgtg gatgcgcttc ggcccaattg
     7441 aggatctgcc gtgccataaa tgacggcata cgaatcacga tcccaaacat tggcgcagtt
     7501 agcgcaatag cgtcacatac acctggatgg cgttgcagaa ataatgtgga gatcgcaccg
     7561 cccatcgaat gtgccagtat atagcgttta cgccacggac cgggctggac ctcctgttgc
     7621 cagaatgccg ccagatcatc aacataatca ttaaagcgat taacatgccc gagatgcgga
     7681 tcggctaaca ggcgaccgga acgtccctgc ccgcgatggt cgatgattaa gacatcaaac
     7741 cccaaatgga acaggtcgta ggccagttcc gcatatttta cgtagctctc aatacgcccc
     7801 gggcagatga ctaccacccg gtcatggtgc tgtgcgcgaa aacggacaaa gcgcaccgga
     7861 atgtcatcca caccagtaaa ctctgcttca tctcgctgac gccagaaatc agtcagcggt
     7921 cccatggtaa aagcagcaaa cgcgttttct cttgtttccc agtctttttg ctgctgaaac
     7981 atcgggtaat ctgcctctta aaccacgtaa aatcgttttt ttaagcgtgc ctgacacaac
     8041 gctgcgacag tagcgtattg tggcacaaaa atagacacac cgggagttca tcatgacctt
     8101 agaatggtgg tttgcctacc tgctgacatc gatcatttta agcctgtcgc caggctctgg
     8161 cgcaatcaac accatgacca cctcgctcaa ccacggttat cgcggcgcgg tggcgtctat
     8221 tgctgggctt cagaccggac tggcgattca tattgtgctg gttggcgtgg ggttggggac
     8281 gctattttcc cgctcagtga ttgcgtttga agtgttgaag tgggcaggcg cagcttactt
     8341 gatttggctg ggaatacagc aatggcgcgc cgctggtgca attgacctta aatcgctggc
     8401 ctctactcaa tcgcgtcgac atttgttcca gcgcgcagtt tttgtgaatc ttaccaatcc
     8461 caaaagtatt gtgtttctgg cggcgctatt tccgcaattc atcatgccgc aacagccgca
     8521 gctgatgcag tatatcgtgc tcggcgtcac gactattgtg gtcgatatta ttgtgatgat
     8581 cggttacgcc acccttgctc aacggattgc tctatggatt aaaggaccaa agcagatgaa
     8641 ggcgctgaat aagattttcg gctcgttgtt tatgctggtg ggggcgctgt tagcatcggc
     8701 gaggcatgcg tgaaaaataa tgtcggatgc ggcgtaaacg ccttatccga cttactctga
     8761 tgacgcgtct ggcatcaacg cgaaataatc aaatgaatgc caaatccggc aaataacgcc
     8821 ccggcaaaac catcaatcca cttcgccaga cgttgataac cacggcgcat ttgcggcagg
     8881 gcaaacaggc tggcaacgac ggtaaaccac gccagtgttt cgataataat caacgcgaaa
     8941 atgccccagc gctcagtggt gccgacgtta tcgccgacaa acagcgagaa taccgaacca
     9001 aagtagataa tcgctttcgg attggcgaga ttggtcagta agcctttcag gaaactgcgt
     9061 ccgctttttg ccagttctac ctgtggcgca ggcgcagaaa cgacctcttt tttcagtgcg
     9121 ctacgcagca tctgataacc catccagcag agatacaggc caccgcccac cataatcagc
     9181 gtatgcagcc aggccatttt ttcgataatc aaatgtaggc caagcaacgc aatcccagcc
     9241 caaaccatta cgccgcaggt aatgcccagc acgcccatca tcgcttcttt acgggaacga
     9301 ctaacagcgg tctgagagac aaaaaagaaa tcgggaccgg ggctcataag cgccacaatg
     9361 tgcaccatgg cgacggtgag gaataacatc aacatacatt agactcgcgg gggaaacgtt
     9421 tacggagtct tcatcctggc acttttttat gctgctgact actcttcgtc atcgccatca
     9481 acatgcgcac gaatcagcgc cataaacggt ttgccaaagc gttccagctt gcgcatccca
     9541 atgccgttaa cgctgagcat ttcgctggca gtgatcggca tctgttccgc catctcaatc
     9601 aaggtcgcgt cgttaaacac cacgtacggc gggacattgc tttcatcggc aatcgattta
     9661 cgcagtttgc gtaatttggc gaacagtttg cgatcgtagt tgccgccgaa ggatttctgc
     9721 atcgctttcg gtttgagcgc cacgatacgt ggcacggcaa gttgcaaaga ggattcgccg
     9781 cgcagcaccg gacgcgcagc ctctgtcagt tgtagggcag aatgctgggc aatattttgc
     9841 gtcaccaggc cgaggtgaat cagctggcgg atcacgctca cccaatgttc atggctttta
     9901 tcacggccca tgccatagac tttcagtttg tcatgaccat agtcgcggat acgctggtta
     9961 ttagcgccac gaatcacttc caccacataa cccatcccaa accgctgatt cacacgacca
    10021 atggtggaaa gggcaatctg agcatcggtt gaaccatcgt attgtttcgg cggatcgagg
    10081 cagatatcgc agttcccgca cggctcctgc cgcccttcgc caaaatagtt tagcagcacc
    10141 agacggcgac aggtttgcgc ttcggcaaac gcgcccatcg cattgagttt atgacgttcg
    10201 atatcctgta actgcccctg cggtttctct tccagacaac gacgcagcca cgccatatcc
    10261 gccggatcgt aaaacagcat cgcttccgcg ggcaggccat cacgcccggc gcgtccggtt
    10321 tcctgataat aggattcaat gttgcgcgga atatcaaaat gcaccacgaa gcgaacgttt
    10381 ggtttattga tgcccatgcc gaacgccacc gtcgccacca caatttgcag gtcatcgcgc
    10441 tggaatttct cctgcacatc agcgcgaaca ttattttcca gcccggcatg ataggccgcc
    10501 gcgctaattc ccttgctttg caggcgcgca gcggtgtctt ctactttcgc acgactatta
    10561 cagtaaataa tccccgactt accgcgttgt tcctgtacat aacgcatcaa ctgatcgagc
    10621 ggtttgaatt tctccatcag catgtagcga atattcggac ggtcaaaact gctgatttga
    10681 atcagcggat cgttcagccc cagcaggcgc acgatatcct ggcgcgtggt gtcatcggct
    10741 gtggcggtca gcgccataaa cggcagcgtc gggaaccgct ggcgcaactg accgagcgcg
    10801 gcatattccg ggcggaaatc gtggccccat tgggagatac agtgcgcttc atcaacggct
    10861 aataacaccg gattccagtg cgccagatgc tcaagaaagt tatccagcat caggcgttcc
    10921 ggcgcgatat aaagcaggcg gatttgcccg gtgcgacagc ctgtcataac ttcaagttgc
    10981 tgctcgcggg tttgcgtcga gttaaggcac gccgccgcca cgccgttggc ttgcagttga
    11041 tccacctgat ctttcatcaa cgaaatcagc ggtgaaacaa ccacggtaag gccgtttagc
    11101 aataaagcag gaatttgata gcaaagggat tttccgccac cagtgggcat gacgaccagg
    11161 caatcgcgac cggaaagcac agtgtcgata atctcttcct ggccggggcg aaactgttgg
    11221 tagccaaagg tttcttgtaa aacctgttta gctccggact ccagattcaa cacttccgcc
    11281 tgcgccacat tcaccccgat tgcctgaaat aaaaacaggc gctattttca gcgcctgcgc
    11341 gagaaactgc aatgctttta gtctctgaga gggtaatcct ggatactgga ttccagattt
    11401 gccaaccact caaccgttca tcaaaacaaa tcgtttagca taacccccac accgacacgg
    11461 gtctggttga agttatagtc gatgagcgat tcgccatagc cgctgtaaac ctgagtataa
    11521 aggcgcacat gtttggtgat cgggtaactt aagcctaact ccgcgccgcc gtagccggtg
    11581 ttccagttgt actgtccttt cgcactcaac accgcatcac cgaggtgata gccgatttta
    11641 agctggtaat agcccatata tttggtgatg tccgggttat cgtccgtatt ccccaccaca
    11701 taccacggct tcacctctac cagccagtta ccgttttctg ccatcaggcg ggtataaagg
    11761 cggttccagc tgcgggaagt cgggtctgaa cgcccgtttg agtcgtggtt gtagcccatt
    11821 tccacatcgc gcagcgtcca gccagcaaag ttgtaatccg tggcaaaacc gaggaacaat
    11881 tgcggttcgt agttagtttc acgaaacggt gaagactctt cgctattgga cagttgccac
    11941 caggattttt gcgtataaga cgcacccaac accgagttcg ggcctaaaat cccacgccac
    12001 agcggaaacg ccaggctcaa ctgaaacttt acttcatcct tacgcgcatt ttccgcccag
    12061 tcgtaactgg caatcgcttc tttattcaga tcgctggttt gggtatagat gatgtagttg
    12121 gtgtcataag gataaagggt gaacggattg tcatgctcct gcagcatatt ggcgataata
    12181 ctgccacgca ccgctggcgc gtcatgcacc tctttcaccg ttgcctcttg tgcatatact
    12241 gccataggca acataaacac cggcaacaac cagccctgca gagtccgcat cgctcgtgtt
    12301 ctccgtaacg aattattgat aaggtgaata tttttctgcg ggctatttta catacttaac
    12361 tccatagccg ttagttatcc cttcctaaag tagaaaccaa caacaaagaa gcataaaatc
    12421 aacatttcat taactaatga agtgtaagga tgatatgtct gccgtactga ccgctgaaca
    12481 agccctgaaa ttagtgggtg agatgtttgt ttatcacatg ccatttaacc gcgcattggg
    12541 gatggaactg gagcgttacg aaaaagagtt cgcacagctg gcctttaaaa atcagccaat
    12601 gatggtgggt aactgggcgc aaagcatttt gcacggcggg gtcattgcgt cggcgctgga
    12661 tgtcgccgcc ggtctggtgt gcgtgggaag taccttaacc cgccacgaaa ccatcagtga
    12721 agatgaacta cgtcagcggc tatcgcgaat ggggaccatt gatcttcgcg tcgattatct
    12781 gcgcccaggc agaggcgagc gttttactgc taccagtagc ctgttgcggg caggcaataa
    12841 agtcgccgtc gcccgcgttg aattacacaa tgaagaacag ctttatattg ccagcgctac
    12901 agccacctat atggtaggtt gataacgtaa aagcaggtaa actgctgtta ccaattaaaa
    12961 atgagtattc ccgatggatg caaaacaaac gcggcagggc gtattactcg ctcttgccgc
    13021 ttattttatt tggggtattg cgccagcgta cttcaagctg atttactacg tgcccgccga
    13081 tgagatcctg acgcatcgcg tgatctggtc gtttttcttt atggtggtgc tgatgagcat
    13141 ctgccgccag tggtcctatt taaaaacgct gattcagacg ccacagaaaa tttttatgct
    13201 ggcagtctct gccgtgctga ttggtggcaa ctggctactg tttatctggg cggtgaacaa
    13261 tcaccatatg ctggaagcga gccttggtta ctttattaac ccgctggtga acattgtgct
    13321 ggggatgatt ttcctcggcg agcgattccg ccggatgcaa tggctggcgg tgattctggc
    13381 gatatgtggc gtgttggttc agttgtggac ttttggttca ctaccgatta tcgcgctggg
    13441 gctggcgttt agttttgcct tctacggcct ggtacgcaag aagattgctg ttgaagcgca
    13501 aaccggcatg ttaatcgaaa ctatgtggtt gctgcccgtg gcggcgattt acctgtttgc
    13561 tattgccgac agctcaacca gccatatggg gcaaaacccg atttcactga atttactgct
    13621 gatcgccgcc gggattgtca ctaccgtacc gctgttgtgt tttactgcgg ctgcgacacg
    13681 cctgcgtctc tcaacgttag gcttttttca gtacattggc ccgacgctga tgttcctgct
    13741 ggctgtgacg ttttatggtg aaaaaccggg tgccgataag atggtgacct tcgcctttat
    13801 ttgggtggcg ctggcaattt ttgtgatgga tgcgatttat acgcagcgta gaacgtccaa
    13861 ataaaaaata aatatattct gaaaaaatat gccgactctt gcatgagagt gggcatattg
    13921 ccatgctgac aggagactca cataaggaaa ggcagaattt ccagacgtcg ttgtatattt
    13981 tcatttgctt taataaattt catttttagt aaattcagca ttatgttatt agcgataagc
    14041 aagaccaatc attaaccaag ctctcttcag aatgtaatga aaaatgtgaa gtgcatttct
    14101 tttttaaaat ttttttaccc tattatttct tatcaaatta acattgccct taattataag
    14161 aaaggagaat ataaatgaac aacatattag gctcatcaat taatatgccc tgtacacttt
    14221 tcgaaactat tactttattc gatgattttt ctgctgatga tatgcaatat ggggatatgg
    14281 aagaacaaga ttttctctca ctcggcttaa gcgacatctc tgcaaaagtt gatccttacc
    14341 gcctcattaa ataccatttt cctgggccga attctactta tggtgctttt agtgttccaa
    14401 cttccggaac aaaaatatca caaagtgaat gtattgatat tttatttgca gaaatgaaag
    14461 atctggcaaa aatgttttcc ttttttggac aatacaaaac actcatccag gatctgattg
    14521 atcattttag atatggaaat ggcaacagtt tccatagcca ggaattgaac ttatcttttc
    14581 atgaacgaat aaacaaatat gattataata gcccaatacg tgtaatcaaa gaatgtatcg
    14641 aaaatgatat ttcttcaaca cctacgacag gataccggcc tcttctccta caaaagatta
    14701 agatcgaatt actttcatca agactaaata aattcaatga ttttaaagat aattttaatg
    14761 gcctgggaat cagcattcat gatatctcag ctcaaaaaat atctctttt
//
LOCUS       DAOTRK010000118        14759 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.118, whole genome shotgun sequence.
ACCESSION   DAOTRK010000118 DAOTRK010000000
VERSION     DAOTRK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.118"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            146..1288
                     /locus_tag="QIE41_003492"
     CDS             146..1288
                     /locus_tag="QIE41_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS3532339.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQQNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1295..2059)
                     /gene="glcC"
                     /locus_tag="QIE41_003493"
     CDS             complement(1295..2059)
                     /gene="glcC"
                     /locus_tag="QIE41_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDS3532340.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNQS"
     gene            2310..3809
                     /gene="glcD"
                     /locus_tag="QIE41_003494"
     CDS             2310..3809
                     /gene="glcD"
                     /locus_tag="QIE41_003494"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDS3532341.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3809..4861
                     /gene="glcE"
                     /locus_tag="QIE41_003495"
     CDS             3809..4861
                     /gene="glcE"
                     /locus_tag="QIE41_003495"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDS3532342.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTSWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4872..6095
                     /gene="glcF"
                     /locus_tag="QIE41_003496"
     CDS             4872..6095
                     /gene="glcF"
                     /locus_tag="QIE41_003496"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDS3532343.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLRKEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALDKE"
     gene            6100..6504
                     /locus_tag="QIE41_003497"
     CDS             6100..6504
                     /locus_tag="QIE41_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDS3532344.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6526..8697
                     /gene="glcB"
                     /locus_tag="QIE41_003498"
     CDS             6526..8697
                     /gene="glcB"
                     /locus_tag="QIE41_003498"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDS3532345.1"
                     /translation="MSQTITQGLLRIDADFKRFVDEEVLPGVELDAAAFWRNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFSRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9052..10734
                     /gene="glcA"
                     /locus_tag="QIE41_003499"
     CDS             9052..10734
                     /gene="glcA"
                     /locus_tag="QIE41_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDS3532346.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSACAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            10888..12219
                     /locus_tag="QIE41_003500"
     CDS             10888..12219
                     /locus_tag="QIE41_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDS3532347.1"
                     /translation="MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTL
                     RKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSE
                     AVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
                     YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLL
                     NAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARL
                     LTVTRKGKVCHLLTSMTDAMRFPGGEMAELYSHRWEIELGYREIKQTMQLSRLTLRSK
                     KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGAS
                     PGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPGNTPQPRKRASQLLN"
     gene            12396..12812
                     /locus_tag="QIE41_003501"
                     /pseudo
     CDS             12396..12812
                     /locus_tag="QIE41_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
     gene            12809..13986
                     /gene="gspL"
                     /locus_tag="QIE41_003502"
                     /pseudo
     CDS             12809..13986
                     /gene="gspL"
                     /locus_tag="QIE41_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
     gene            13988..14524
                     /gene="yghD"
                     /locus_tag="QIE41_003503"
     CDS             13988..14524
                     /gene="yghD"
                     /locus_tag="QIE41_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDS3532348.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSAAPNIIAARKSGTTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLNWLKALDEKYALRVAQIDVS
                     AGEKPGMVNVQRLEFGRG"
BASE COUNT         3480 a         3712 c         4160 g         3407 t
ORIGIN      
        1 gcttctgtgt agtctctgat cttatcttaa ttattaataa atactattta ttctttgttt
       61 gtattcattg ggttgattat taaggtgccg ttcgttgctg ttgcaacctg tttgtcaccg
      121 ttaattattt gttacccaca gaactatgga atgcgatctg ctgatgatta aaattgaaaa
      181 agtaattaat aaaaatgacc ttaaggcatt tatcgctttt ccgtcatcac tttatcccga
      241 cgatccaaac tggatacccc ctttattcat tgaacgcaac gagcatttgt ctgcgaaaaa
      301 tccggggacg gaccatatca tctggcaggc gtgggtggca aaaaaagcgg ggcagatagt
      361 tgggcgtatt accgcgcaaa tagatacctt gcaccgcgag cgttacggtg aagataccgg
      421 tcatttcggc atgattgacg ccattgatga tccgcaggtt tttgccgcgt tgtttggcgc
      481 agcggaagcg tggttgaagt cacaaggagc aagtaagatc agcgggcctt tcagcctgaa
      541 tatcaatcag gaaagcggat tactgattga aggtttcgat acaccaccct gtgcgatgat
      601 gccgcacggt aaaccgtggt atgccgcgca tattgaacaa ttgggttatc acaaaggcat
      661 tgatttactg gcgtggtgga tgcagcgaac cgatctcact ttctctccgg cgctaaaaaa
      721 actgatggat caggtgcgta agaaggtgac cattcgctgc atcaatcgcc agcggtttgc
      781 cgaagagatg cagatcctgc gtgagatttt caactctggc tggcagcaaa actggggatt
      841 tgtgccgttt accgaacatg aattcgcgac catgggcgat caacttaaat atctggtgcc
      901 ggacgatatg atctacatcg ctgagattga ttctgcaccc tgcgcgttta ttgtcggctt
      961 gccaaacatc aacgaggcga ttgccgatct gaacggatcg ctctttccct tcggctgggc
     1021 aaaattgctg tggcgtttaa aagtcagcgg tgtgcgaact gcgcgagtac cgctgatggg
     1081 cgtgcgggac gagtatcagt ttagccgcat cggcccggtg attgcgctgt tattgattga
     1141 agccttacgc gatccgtttg cccgccggaa gatcgatgcg ctggaaatgt catggatcct
     1201 cgaaaccaat acaggcatga ataacatgct ggaacgcatt ggggcggagc cgtacaagcg
     1261 ttaccgattg tatgaaaagc agatttaata ccagctaact ctggttcatc tccagcggca
     1321 ccgccgagcg aatcaaatgg tggccttcca gctcgatatc gtggagattc tttttcacgg
     1381 tccgcacatg atcgcgtgct gcgcgctggg cgacgtgcgg cagccgctgc aacaccgcgt
     1441 tgtagatccg cgcatgctgg cgatcgatct gctttttttg ctgtggtcga tggtagagat
     1501 tatttacgct ggcaaacact gaattaaaca tcaggtcggt caatgattgc agtgtaaaca
     1561 ccaacacctg attgtgagaa gcctgacaaa tggcgagatg gaaagcgtga tccaactgcg
     1621 catgttcgat cagcgaaatc tctttgttgt tctcactggc ggcgagcatt ttttcataac
     1681 agcgggttat cacaacaaaa tcagcctgcg ttcccagcgt tgccgccagc cttgccgatt
     1741 cgccctccag taatgcgcga acgtcgagca gatcgtacag cgttcgcggc tgcgtactga
     1801 acagatggat cagcgggctg gtgtcctgca cccgattaag ccgtgcgaca cgagaatcgc
     1861 gaccctgcgc cgtttcaata atcccgcgcc cgcgcagcac ggtcagccct tcacgcagtg
     1921 cggagcgtga gaagccgagc ttttcacaca gtcgacgttc cgagggaagc ggctgaccga
     1981 ccttcagtac gccgtcgata attaaccgtt cgatactctc tgcaaccact tcgcaaatag
     2041 ggcgacgttc atctttcatt cccggacctc gtgcacaggt aggaccaatt tttctgttct
     2101 tgactcgcaa aacacgcaca tcacgtaagt gattatgtta catcaattta acattgagtt
     2161 aaccaagaca aggtcacaga gctggaaaaa aaatggtctg accggtagct aaagagatag
     2221 acgaaaacga aaagcccgct taataactgt tcacagaagc agcgcgcaaa aatcagctgc
     2281 cacacaacac aacaaagcga agcctactca tgagcatctt gtacgaagag cgtcttgatg
     2341 gcgctttacc cgatgtcgac cgcacatcgg tactgatggc actgcgtgag catgtccctg
     2401 gacttgagat cctgcatacc gatgaggaga tcattcctta cgagtgtgac gggttgagcg
     2461 cgtatcgcac gcgtccatta ctggttgttc tgcctaagca aatggaacag gtgacagcga
     2521 ttctggctgt ctgccatcgc ctgcgtgtac cggtggtgac ccgtggtgca ggcaccgggc
     2581 tttctggtgg cgcgctgccg ctggaaaaag gtgtgttgtt ggtgatggcg cgctttaaag
     2641 agatcctcga cattaacccc gttggtcgcc gcgcgcgcgt gcagccaggc gtgcgtaacc
     2701 tggcgatctc ccaggccgtt gcaccgcata atctctacta cgcaccggac ccttcctcac
     2761 aaatcgcctg ttccattggc ggcaatgtgg ctgaaaatgc cggcggcgtc cactgcctga
     2821 aatatggtct gaccgtacat aacctgctga aaattgaagt gcaaacgctg gacggcgagg
     2881 cactgacgct tggatcggac gcgctggatt cacctggttt tgacctgctg gcgctgttca
     2941 ccggatcgga aggtatgctc ggcgtgacca ccgaagtgac ggtaaaactg ctgccgaagc
     3001 cgcccgtggc gcgggttctg ttagccagct ttgactcggt agaaaaagcc ggacttgcgg
     3061 ttggtgacat catcgccaat ggcattatcc ccggcgggct ggagatgatg gataacctgt
     3121 cgatccgcgc ggcggaagat tttattcatg ccggttatcc cgtcgacgcc gaagcgattt
     3181 tgttatgcga gctggacggc gtggagtctg acgtacagga agactgcgag cgggttaacg
     3241 acatcttgtt gaaagcgggc gcgactgacg tccgtctggc acaggacgaa gcagagcgcg
     3301 tacgtttctg ggccggtcgc aaaaatgcgt tcccggcggt aggacgtatc tccccggatt
     3361 actactgcat ggatggcacc atcccgcgtc gcgccctgcc tggcgtactg gaaggcattg
     3421 cccgtttatc gcagcaatat gatttacgtg ttgccaacgt ctttcatgcc ggagacggca
     3481 acatgcaccc gttaatcctt ttcgatgcca acgaacccgg tgaatttgcc cgcgcggaag
     3541 aactgggcgg gaagatcctc gaactctgcg ttgaagttgg cggcagcatc agtggggaac
     3601 atggcatcgg gcgcgaaaaa atcaatcaaa tgtgcgccca gttcaacagc gatgaaatca
     3661 cgaccttcca tgcggtcaag gcggcgtttg accccgatgg tttgctgaac ccaggaaaaa
     3721 acattcccac gctacaccgc tgtgctgaat ttggtgccat gcatgtgcat cacggtcatt
     3781 taccttttcc tgaactggag cgtttctgat gctacgcgag tgtgattaca gccaggcgct
     3841 gctggagcag gtgaatcagg cgattagcga taaaacgccg ctggtgattc agggcagcaa
     3901 tagcaaagcc tttttaggtc gccctgtcac cgggcaaacg ctggatgtgc gttgtcatcg
     3961 cggcattgtt aattacgacc cgaccgagct ggtgataacc gcgcgtgccg gaacgccgct
     4021 ggtggcgatt gaagcagcgc tggagagcgc cgggcaaatg ctcccctgcg agccgccgca
     4081 ttatggtgaa gaagccacct ggggcgggat ggtcgcttgc gggctggcgg ggccgcgtcg
     4141 cccgtggagt ggttcggtcc gcgattttgt cctcggcacg cgcatcatta ccggcactgg
     4201 aaaacatctg cgttttggtg gcgaagtgat gaaaaacgtt gccggatacg atctctcacg
     4261 gttaatggcc ggaagctacg gttgtcttgg cgtgctcact gaaatctcga tgaaagtgtt
     4321 accgcgaccg cgcgcctccc tgagcctgcg tcgggaaatc agcctgcaag aagccatgaa
     4381 tgaaatcgcc cagtggcaac tccagccgct acccattagt ggcttatgtt acttcgacaa
     4441 tgcgttgtgg atccgccttg agggcggcga aggatcggta aaagcagcgc gtgaactgct
     4501 gggtggcgaa gaggttgcag gtcagttctg gcagcaattg cgtgaacaac aactgccgtt
     4561 cttctcgtta ccaggtacct catggcgcat ttcattaccc agtgatgcgc cgatgatgga
     4621 tttacccggc gagcaactga tcgactgggg cggggcgtta cgctggctga aatcgacagc
     4681 cgatgataat caaatccatc gcatcgcccg caacgcgggc ggtcatgcga cccgctttag
     4741 tgccggagac ggtggctttg ccccgctacc ggcaccttta ttccgctatc accagcagct
     4801 taaacagcag ctcgatcctt gcggcgtgtt taaccccggt cgcatgtacg cggaactttg
     4861 aggagcaggc tatgcaaacc cgattaacag aagagatgcg gcagaacgcg cgcgcgctgg
     4921 aagccgacag catcctgcgc gcctgtgttc actgcggatt ttgtaccgca acctgcccaa
     4981 cctatcagct tctgggcgat gaactggacg ggccgcgcgg gcgcatctat ctgattaaac
     5041 aggtgctgga aggtaacgaa gtcacgctta aaacacagga gcatctcgat cgctgtctca
     5101 cttgccgcaa ttgtgaaacc acctgtcctt ctggtgtgcg ttatcacaat ttgctggata
     5161 tcgggcgtga tattgtcgag cagaaagtga aacgcccact gccggagcga atgctgcgcg
     5221 aaggattgcg ccaggtagtg ccgcgtccgg cggtcttccg tgcgctgacg caggtagggc
     5281 tggtgctgcg accgttttta ccggaacagg tcagagcaaa actgcctgct gaaaccgtga
     5341 aagctaaacc gcgtccaccg ctgcgccata agcgtcgggt tttaatgctg gaaggctgcg
     5401 cccagcctac gctttcgccc aacaccaacg cggcaactgc gcgagtgctg gatcgtctgg
     5461 ggatcagcgt catgccagct aacgaagcag gctgttgtgg cgcggtggac tatcatctta
     5521 atgcgcaaga gaaagggtta gcgcgggcgc gcaataatat tgatgcctgg tggcccgcga
     5581 ttgaagcagg tgccgaggca attttgcaaa ccgccagcgg ttgcggcgcg ttcgttaaag
     5641 agtatgggca gatgctgaaa aacgatgcgt tgtatgccga taaagcgcgt caggtcagtg
     5701 aactggcggt cgatttagtc gaacttctgc gcaaggaacc gctggaaaaa ctggcaattc
     5761 gcggcgataa aaaactggcc ttccactgcc cgtgtaccct gcaacatgcg caaaagctga
     5821 acggcgaagt ggaaaaagtg ttacttcgtc ttggatttac cttaacggac gttcccgaca
     5881 gccatctgtg ctgcggttca gcgggaacat atgcgttaac gcatcccgat ctggcacgcc
     5941 agctgcggga taacaaaatg aatgcgctgg aaagcggcaa accggaaatg atcgtcaccg
     6001 ccaacattgg ttgccagacg catctggcga gcgccggtcg tacctctgtg cgtcactgga
     6061 ttgaaattgt agaacaagcc cttgataagg aataacaaaa tgaaaactaa agtcattctt
     6121 agccagcaaa tggcgagtgc aattattgcc gcaggtcagg aagaggcgca gaaaaataac
     6181 tggtctgttt ccattgctgt tgccgatgac ggcggtcatc tgctggcgtt aagtcgcatg
     6241 gatgattgcg cgccaattgc ggcttacatc tcccaggaga aagcgcgtac cgccgcgctg
     6301 ggacgtcgtg aaactaaggg ctatgaagag atggtgaaca acggacgtac cgcgttcgtg
     6361 actgcgccgt tactgacgtc gctggaaggc ggcgtaccgg ttgttgtgga tgggcaaatt
     6421 attggtgccg tgggcgtttc tggtttaacc ggagcacagg atgcgcaggt cgcgaaagcg
     6481 gcagcagcgg tgttggcgaa ataagcgaaa acgaggagat aaacaatgag tcaaaccata
     6541 acccagggcc ttttacgcat tgacgccgac tttaaacgtt ttgtggatga agaagttttg
     6601 ccgggcgtag aactggatgc tgccgcgttc tggcgcaatg ttgatgagat tgttcacgat
     6661 ctggcaccag aaaatcgtca gttgctggca gaacgcgatc gcattcaggc agcgcttgat
     6721 gagtggcatc gcagcaatcc ggggccggta aaagataaag cggcctataa atctttcctg
     6781 tgtgaactgg gctacctggt gccgcaaccg gagcgcgtga cggtggaaac cacgggcatt
     6841 gacagcgaaa tcaccagcca ggcggggcca cagctggtgg ttccggcaat gaatgcccgc
     6901 tacgcgctga acgcggcgaa cgcgcgctgg ggctcactgt acgatgcgtt atacggcagc
     6961 gacatcatcc cgcaggaagg ggcgatggtc agcggctacg atccgcagcg cggtgagcag
     7021 gttatcgcct gggttcggcg tttcctcgat gaatcactac cgctggaaaa tggcagctat
     7081 caggatgtgg tggcgtttaa ggtggtcgat aaacaattac gcatccagtt gaaaaatggt
     7141 aaagaaacca cgttacgtac tccagcacag tttgtcggtt accgtggcga tactgctgcg
     7201 ccgacctgca ttttgctgaa aaataacggc ctgcatattg agctacaaat cgatgccaac
     7261 gggcggattg gcaaagacga ttcggcgcac atcaacgatg ttatcgtcga agcggccatc
     7321 agtaccattc tcgactgcga agattcggtt gcggcggttg atgcggaaga taaaatcctg
     7381 ctgtaccgca acctgctggg cctgatgcag gggactctgc aagagaaaat ggagaagaac
     7441 ggtcggcaaa tcgtgcgtaa actgaatgac gatcgtcagt acactgccgc cgatggatct
     7501 gaaatttctc tgcacggacg ctcgttgctg tttatccgca acgtggggca tttgatgacc
     7561 attcctgtga tttgggacag cgaaggcaat gaaatcccgg aaggcattct tgatggcgtc
     7621 atgactggcg cgattgccct ctatgattta aaagtgcaga aaaactcgcg cactggcagc
     7681 gtctatattg tgaaaccgaa aatgcacggt ccgcaggaag tggcgttcgc caacaaactg
     7741 ttttcccgcg ttgagacaat gctcggtatg gcaccgaata ccctgaaaat gggcattatg
     7801 gatgaagaac gtcggacctc gctgaacttg cgtagctgta tcgctcaggc gcgcaaccgc
     7861 gtggcgttca tcaataccgg tttcctcgac cgtaccggcg atgaaatgca ttcggtgatg
     7921 gaagctggcc cgatgctgcg taaaaaccag atgaaatcga cgccgtggat caaagcctac
     7981 gagcgtaata acgtgctttc cggtctgttc tgtgggctgc gcggtaaagc gcaaattggt
     8041 aaaggcatgt gggcaatgcc ggacctgatg gcagacatgt acagccagaa gggcgaccaa
     8101 ctgcgtgccg gggcaaacac agcctgggtt ccgtcaccaa ccgctgctac gctccatgcg
     8161 ctgcactacc accaaaccaa cgtacagagc gtgcaggcca acattgccca gagcgagttc
     8221 aatgctgaat ttgaaccgtt gctggacgat ctgctgacta ttccggtggc tgaaaacgct
     8281 aactggtcgg tggaagagat ccaacaagag ctggataaca acgtgcaggg gattctgggg
     8341 tacgtggtgc gttgggtgga gcaggggatt ggttgttcaa aagtgccgga tattcacaac
     8401 gtggcgctga tggaagaccg cgcaacgctg cgtatctcca gccagcatat cgccaactgg
     8461 ttacgtcacg gtattctgac caaagaacag gtgcaggcgt cgctggagaa tatggcgaaa
     8521 gtggttgatc agcaaaacgc tggcgatccg gcttatcgtc cgatggcggg gaatttcgct
     8581 aactcgtgtg cttttaaagc tgccagcgat ttaatcttcc tcggcgtgaa acagccaaac
     8641 ggctataccg aaccgttatt acacgcctgg cgtttacgcg aaaaagaaag tcattaatag
     8701 cgtcaaaaaa gccccggcaa gttttattgt cggggctatg atagtaactg cggtagtaca
     8761 tttatcccgg aaataaccgg agcgattatc agggtttaac aatattaaat acccgtagcg
     8821 cattactagc gggcctaaaa caacaatatg gacatcttaa cgatattctg cgcctgcgtg
     8881 tgcggaacag aaacacttac ccgatatact tgttatgcca gaggtagcaa ttgcgttagc
     8941 agcgtactgt tgctgtaaag tataacgcct atattttaca gttcagtcct cttttgctta
     9001 ttacaacctt aagtatatca agcatataaa gataataaga gactgaacaa tatggttacc
     9061 tggacccaaa tgtatatgcc gatgggagga ctggggctat ccgctctggt cgccctgatc
     9121 ccgataatat tcttcttcgt tgcactcgcg gtattacgtc tgaaaggaca tgtcgccgga
     9181 gcaataaccc ttatattatc tatcctgatt gcaatattcg cctttaaaat gccgattgat
     9241 atggcatttg ctgctgcggg ctatggcttt atttatggat tatggcctat tgcgtggatt
     9301 attgttgcgg cggtgttcct ctataaatta accgttgcca gcggtcagtt cgatattatc
     9361 cgcagctcgg ttatctccat cactgacgat cagcgtttgc aggtgttact gattggtttc
     9421 tcctttggcg cgctgctgga aggggcggct ggctttggtg cgccggtggc gattaccggt
     9481 gcgctgctgg tgggcctggg cttcaaaccg ttatacgcgg cggggctgtg tctgattgcc
     9541 aatactgcgc cggtggcgtt tggtgcgttg ggcgtgccga ttctggtcgc cggtcaggta
     9601 acgggaatcg atccgttcca cattggcgca atggcgggac gtcagttacc gttcctgtcg
     9661 gttcttgtgc cgttctggct ggtagcaatg atggacggct ggaaaggggt gaaagagacg
     9721 tggccagcgg cgctggttgc tgggggaagc ttcgctgtca cgcagttctt tacctctaac
     9781 tatattggtc cggaactgcc ggatattact tcggcgctgg tgagtatcgt ctcactcgct
     9841 ttattcctta aagtctggcg gccgaaaaat accgaaacgg caatcagcat gggacaatcc
     9901 gcatgtgcga tggtggtgaa taaaccatct tctggcggtc ccgtgccttc agaatatagt
     9961 ctggggcaaa tcattcgtgc gtggtcaccg tttttaatct taacggtgtt ggtcaccatc
    10021 tggaccatga agccgtttaa agcgttattt gctccgggcg gcgcgtttta ttcactggtg
    10081 attaatttcc agatccctca tttgcatcaa caagtcttga aagcggcccc cattgtcgcc
    10141 cagccgacgc caatggacgc ggtgtttaaa ttcgaccccc tctctgctgg tggcaccgct
    10201 atttttattg cggcgattat ctctatcttc atcctcggcg tggggatcaa gaaaggtatt
    10261 ggcgtctttg ccgaaacgct aattagtctg aagtggccga tactgtcgat tggcatggtg
    10321 ctggcatttg ccttcgtcac caactattcc ggtatgtcca ccacgctggc gctggtactg
    10381 gctggtacag gcgtgatgtt cccattcttc tcaccgtttc tcggctggct gggcgtattc
    10441 cttaccggct cggacacttc ttcaaacgcc ctgtttggtt cactgcaatc gaccacggcg
    10501 cagcaaatca acgtctctga caccctgctg gtggcagcaa acaccagcgg cggcgtaact
    10561 ggcaagatga tctccccgca atctatcgcc gtggcctgcg ccgcgacggg catggtgggt
    10621 cgagaatctg aactgttccg ctacaccgtg aagcacagtc tgatttttgc cagcgttatc
    10681 ggcattatca cgctgttgca ggcgtatgtg tttaccggga tgttagtttc gtaagtcaat
    10741 aaagccggat gatcgttaat atcatccggc ttcgttgtgc acagaaaacc cccagctagg
    10801 ctgtaatgcc gatcagttaa ggatcagttg accgatccag tggctgtgta agaatccgga
    10861 aacgctcact tgtttccgga tttttttatg cacattggac aggctcttga tctggtatcc
    10921 cgttacgatt ctctgcgtaa cccactgact tctctggggg attacctcga ccccgaactc
    10981 atctctcgtt gccttgccga atcaggtact gtaacgctac gcaagcgccg tcttcccctc
    11041 gaaatgatgg tctggtgtat tgttggcatg gcgcttgagc gtaaagaacc tcttcaccag
    11101 attgtgaatc gcctggacat catgctgccg ggcaatcgcc ccttcgttgc ccccagtgcc
    11161 gttattcagg cccgccagcg cctgggaagt gaggctgtcc gccgcgtgtt cacgaaaaca
    11221 gcgcagctct ggcataacgc cacgccgcat ccgcactggt gcggcctgac cctgctggcc
    11281 atcgatggtg tgttctggcg cacaccggat acaccagaga acgatgcagc cttcccccgc
    11341 cagacacatg ccgggaaccc ggcgctctac ccgcaggtca aaatggtctg ccagatggaa
    11401 ctgaccagcc atctgctgac ggctgcagcc ttcggcacga tgaagaacag cgaaaatgag
    11461 cttgctgagc aacttataga acaaaccggc gataacactc tgacgttaat ggataaaggt
    11521 tattactcac tgggactgtt aaatgcctgg agcctggcgg gagaacaccg ccactggatg
    11581 atacctctca gaaagggagc gcaatatgaa gagatcagaa aactgggtaa aggcgatcat
    11641 ctggtgaagc tgaaaaccag cccgcaggca cgaaaaaagt ggccgggact gggaaatgaa
    11701 gtgacagccc gcctgctgac cgtgacgcgc aaaggaaaag tctgccatct gctgacgtcg
    11761 atgacggacg ccatgcgctt ccccggagga gaaatggcgg aactgtacag tcatcgctgg
    11821 gaaatcgaac tgggatacag ggagataaaa cagacgatgc aactgagcag gctgacgctg
    11881 agaagtaaaa aaccggagct tgtggagcaa gagctgtggg gtgtcttact ggcttataat
    11941 ctggtgagat atcagatgat taaaatggcg gaacatctga aaggttactg gccgaatcaa
    12001 ctgagtttct cagaatcatg cggaatggtg atgagaatgc tgatgacatt gcagggcgct
    12061 tcaccgggac gtataccgga gctgatgcgc gatcttgcaa gtatgggaca acttgtgaaa
    12121 ttaccgacaa gaagggaaag ggccttcccg agagtggtaa aggagaggcc tggaaatacc
    12181 ccacagcccc gaaaaagagc cagtcagttg cttaactgac tggcattaca gctaggctgg
    12241 gggttccgga aagctttcag ctttgagcca gttattaaaa ccccttttga tttgttaaaa
    12301 caccttgcgg tctggcaact gcaagtgtca aacaagaaat caaaaggggg tcccaatggg
    12361 gaacgaaaag agcttagcgc acacccgatg gaacgcgcgt ggtacaggga atggacgccg
    12421 ggctttatca aaaactgaaa ccgttggtct gtgcgctgcc gatggcccgc cagcaaatca
    12481 acatcaatac attagatgtc acgcaaagtg tgattcttga ggcgctgttt gacccgtggt
    12541 taagccctgt tcaggcgcgg gcgttattac aacaacgtcc ggcgaagggc tgggaagatg
    12601 tcgatcagtt tcttgcacag ccgctacttg cagacgtcga tgagcgtact aaaaaacagc
    12661 taaaaaccgt cctgagcgtg gacagcaatt acttctggct gcgttcagat atcaccgtga
    12721 atgagattga actgacgatg aattcgttaa ttgtccgcat gggcccacaa cacttttctg
    12781 ttctctggca tcagacagga gaaagtgagt gagtttcatg cttgagattt ttttcccgct
    12841 ttgcgccgct gatcccatcc gttggcagcg ccatacaccc gacgtggagc acggtatctg
    12901 gcctgacgtt gctgatgaat gtctccagca atggctgcaa actgatgcga ttcgactcta
    12961 cattcccggc gaatggatca gcgtctggca ggttgaactg cctgatgtgc cccgcaagca
    13021 gataccgacg attctgcccg ccttgctgga agaagagctg aaccaggata tcgatgaact
    13081 gcattttgcc ccgttgaaaa tcgaccagca actggcaacc gtagctgtga ttcaccagca
    13141 gcatatgcgc aacattgcgc agtggttgca ggcaaacggc atcacccgcg ctaccgtcgc
    13201 gccggactgg atgtccattc cttgtgggtt tatggctggc gatgcgcaac gggttatctg
    13261 ccgcatcgat gaatgccggg gatggagcgc cgggcgggcg ctggctccgg tcatgttccg
    13321 cgcacagctc aatgagcaga atttaccgat ttcactaacc gtggtcggca ttgcaccgga
    13381 agaactgtct gcatgggctg gtgcggacgc gaacgcctga ccgttacggc tctgcccgcc
    13441 attaccactt atggcgaacc ggaagggaac ctgctaacag ggccgtggca gcctcgtgtc
    13501 agctaccgaa aacagtgggc gcgctggcgg gtgatgattc tgccgatatt gctgattctg
    13561 gttgcgctgg tagtggaacg gggcgtgacg ttatggagcg tcagcgaaca ggtggcgcaa
    13621 agccgcaccc aggcggagaa acagttctta acgctattcc tggagcagaa gcggattgtg
    13681 aatttacgct ctcagatgac gatggcgctg aaaaaatatc gcccacaggc cgacgatacc
    13741 tggctgctcg cagaattgtc agtgatcgcc agtaccctga aatcagcgtc actttccgac
    13801 atcgaaatgc gtggtttcac ctttgatcaa aaacgccaga cgcttcacct tcagctacgg
    13861 gctgcgaact ttgccagctt cgacaaactg cgtagcgcac tggcggcaga ttatgttgtg
    13921 caacaggacg cgttacagaa agagggtgat gcggtttccg gcggcgtaac gttgcggagg
    13981 aaataacatg ttacgcgata aatttattca ctattttcag caatggcgtg aacgccagtt
    14041 aagccgtggc gaacactggc tgacacaaca cctggcgggg cgttctccgc gtgaaaaagg
    14101 catgttactg gcagcggtag tgttcctgtt tagcgccgga tattacgtcc tcatctggca
    14161 gccgttgagc gaacggatag aacaacagga gacgatgttg cagcagctgg tggcgatgaa
    14221 cgcgcgactg aagagcgccg cgccgaatat tattgcggcg cgaaaatccg gcacgacaac
    14281 gccagcacag gtatcgcggg tcatcagcga cagcgcttcc gcgcactctg tggtcatcaa
    14341 acggattgcc gagcgtgggg agaatattca ggtctggata gaacctgtgg tgtttaatga
    14401 cctgctgaac tggttaaagg cgctggatga aaagtacgcg ctgcgggtgg cacaaattga
    14461 tgtcagtgcg ggtgagaagc ctgggatggt gaatgtgcag cgactggagt ttgggcgggg
    14521 gtaggtattg ccatttcctt aaccccacct gataaccctt agcccaatgg tgagtgcgga
    14581 actgtcaaac ccgctcaccg ggctgagttt cgatgtagca agaattatct ccctccagac
    14641 caccacggaa tattcggtaa cactgaagga acctgttttt caccatgtag ccttcgttaa
    14701 tcgacacctt tctcagactg taaagttgct taagtgtttt gacacattat gattattaa
//
LOCUS       DAOTRK010000119        14595 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.119, whole genome shotgun sequence.
ACCESSION   DAOTRK010000119 DAOTRK010000000
VERSION     DAOTRK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.119"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..465
                     /locus_tag="QIE41_003504"
     CDS             <1..465
                     /locus_tag="QIE41_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDS3532349.1"
                     /translation="FAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADE
                     IAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGE
                     ATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            621..2156
                     /gene="gadC"
                     /locus_tag="QIE41_003505"
     CDS             621..2156
                     /gene="gadC"
                     /locus_tag="QIE41_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDS3532350.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAVVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKGNTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2287..3606
                     /locus_tag="QIE41_003506"
     CDS             2287..3606
                     /locus_tag="QIE41_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDS3532351.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPASRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3904..3945
                     /locus_tag="QIE41_003507"
     CDS             3904..3945
                     /locus_tag="QIE41_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532352.1"
                     /translation="MVQLVDLARCVSF"
     gene            3983..5365
                     /gene="dosC"
                     /locus_tag="QIE41_003508"
     CDS             3983..5365
                     /gene="dosC"
                     /locus_tag="QIE41_003508"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDS3532353.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5390..7789
                     /gene="dosP"
                     /locus_tag="QIE41_003509"
                     /gene_synonym="pdeO"
     CDS             5390..7789
                     /gene="dosP"
                     /locus_tag="QIE41_003509"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDS3532354.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKIYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDSLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGINGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8047..8628
                     /gene="ddpX"
                     /locus_tag="QIE41_003510"
     CDS             8047..8628
                     /gene="ddpX"
                     /locus_tag="QIE41_003510"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDS3532355.1"
                     /translation="MSDTTVLVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVAVGSNHSRGT
                     AIDLTLRDEHGNILDMGAEFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHAS"
     gene            8642..10192
                     /locus_tag="QIE41_003511"
     CDS             8642..10192
                     /locus_tag="QIE41_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDS3532356.1"
                     /translation="MKRSISFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVRFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10194..11216
                     /locus_tag="QIE41_003512"
     CDS             10194..11216
                     /locus_tag="QIE41_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3532357.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11213..12109
                     /locus_tag="QIE41_003513"
     CDS             11213..12109
                     /locus_tag="QIE41_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDS3532358.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12106..13092
                     /locus_tag="QIE41_003514"
     CDS             12106..13092
                     /locus_tag="QIE41_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3532359.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNHRCACWYPQQEVISV"
     gene            13085..14011
                     /locus_tag="QIE41_003515"
     CDS             13085..14011
                     /locus_tag="QIE41_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3532360.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDVQQVLTAPAHPYTRLLLDSLPAINKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(14067..14498)
                     /gene="osmC"
                     /locus_tag="QIE41_003516"
     CDS             complement(14067..14498)
                     /gene="osmC"
                     /locus_tag="QIE41_003516"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDS3532361.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3573 a         3647 c         3716 g         3659 t
ORIGIN      
        1 ttcgccatca acttctcccg cccggcgggt caggtaattg cacagtacta tgaattcctg
       61 cgcctcggtc gtgaaggcta taccaaagta cagaacgcct cttaccaggt tgccgcttat
      121 ctggcggatg aaatcgccaa actggggccg tatgagttca tctgtaccgg tcgcccggac
      181 gaaggcatcc cggcggtttg cttcaaactg aaagagggtg aagatccggg atacaccctc
      241 tacgacctct ctgaacgtct gcgtctgcgc ggctggcagg ttccggcctt cactctcggc
      301 ggtgaagcca ccgacatcgt ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac
      361 tttgctgaac tgttgctgga agactacaaa gcctccctga aatatctcag cgaccacccg
      421 aaactgcagg gtattgccca gcagaacagc tttaaacata cctgataacg tttaacggta
      481 acggtgtccc gaaacacacc cgtttcggga caatttccaa agtctgttca ctggcattag
      541 caacggaaaa tattgttctg aatacgtttc agaacaaaac aggtgcggtt ccgacaggaa
      601 taccgtttta gggggataat atggctacat cagtacagac aggtaaagct aagcagctca
      661 cattactcgg attttttgcc ataacggcat cgatggtaat ggctgtttat gaatacccta
      721 ccttcgcaac atcgggcttt tcattagtct tcttcctgct attaggcggg attttatggt
      781 ttattcccgt gggactttgt gctgcggaaa tggctaccgt cgacggctgg gaagaaggtg
      841 gtgtcttcgc ctgggtatca aatactctgg ggccgagatg gggatttgca gcgatctcat
      901 ttggctatct gcaaatcgcc attggtttta ttccgatgct ctatttcgtg ttaggggcac
      961 tctcctacat cctgaaatgg ccagcgctga atgaagaccc cattaccaaa actattgcag
     1021 cactcatcat tctttgggcg ctggcattaa cgcagtttgg tggcacgaaa tacacggcgc
     1081 gaattgctaa agttggcttc ttcgccggta tcctgttacc tgcatttatt ttgatcgcat
     1141 tagcggctat ttatctgcac tccggtgccc ccgttgctat cgaaatggat tcgaagacct
     1201 tcttccctga cttctctaaa gtgggcaccc tggttgtatt tgttgccttc attttgagtt
     1261 atatgggggt agaagcatct gcaacccacg tcaatgaaat gagcaaccca gggcgcgact
     1321 atccgttggc tatgttattg ctgatggtgg cggcaatctg cttaagctct gttggcggtt
     1381 tgtctattgc gatggtcatt ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa
     1441 cctttaccgt tctgatgtcc catgtggcac cggaaattga gtggacggtt cgcgtgatct
     1501 ccgcactgct gttgctgggt gttctggcgg aaatcgcttc ctggattgtt ggtccttctc
     1561 gcgggatgta tgtcacagcg cagaaaaacc tgctgccagc agcattcgct aaaatgaaca
     1621 aaaatggcgt accggtaacg ctggtcattt cgcagctggt gattacttct atcgcgttga
     1681 tcatcctcac caataccggt ggcggtaaca acatgtcctt cctgatcgca ctggcgctga
     1741 cggtggtgat ttatctgtgt gcttatttca tgctgtttat tggctacatt gtgttggttc
     1801 ttaaacatcc tgacttaaaa cgcacattta atatccctgg tggtaaaggg gtgaaactgg
     1861 tcgtggctgt tgtcggtctg ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc
     1921 cggataacat ccagggtgac tctaccgata tgtatgttga attactggtc gtgagtttcc
     1981 tggtggtact tgccctgccc tttattctct atgctgttca tgatcgtaaa ggcaaaggga
     2041 ataccggcgt cactctggag ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc
     2101 tgcacccgcg tgcacgttca ccacactata ttgtgatgaa tgacaagaaa cactaaccat
     2161 gtctgaatgg ttataagata agggagcgat aattcatcgc tccctttttc gtgcttgctg
     2221 tcgtattgac ttaaccggga taagtacgag aatgagcgca catctgttta ccggaaacca
     2281 gcacatatgg atatctgctc ccgaaacgag aaattagcga ttagaagacc agcgatactg
     2341 gttgcactgg cacttttact gtgtagttgt aaaagcacgc ctccagagtc catggtgaca
     2401 ccaccagcgg gttcaaagcc accagccacg acgcaacaat cgtcacaacc gatgcgtggc
     2461 atctggctgg ctacggtgtc tcgcctcgac tggccaccgg tttcctcggt taacattagt
     2521 aaccccacca gccgggcccg tgtacaacaa caggcgatga tcgacaaact ggatcatctg
     2581 caacgtctcg gcataaacac ggtctttttc caggtcaagc cggacggtac cgccctgtgg
     2641 ccatcaaaaa ttttgccgtg gtccgatctt atgaccggaa agattggtga aaatccaggt
     2701 tacgatccgc tgcaatttat gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc
     2761 tggtttaacc cctatcgcgt atcggttaat acgaagcccg gtactatcag ggaactgaat
     2821 agcactctgt ctcaacaacc agcgagcgtc tatgtgcaac accgcgactg gatcagaacg
     2881 tccggcgatc gctttgtcct cgacccgggc atccctgaag ttcaggactg gatcacatca
     2941 atagtcgcgg aagtggtttc ccgctatccg gtagatggcg tgcagtttga cgactatttc
     3001 tatactgagt caccagcttc acggctaaat gataacgaaa cgtaccgcaa atacggaggc
     3061 gcatttgcgt caaaagcaga ctggcggcgc aacaatactc agcagttaat tgcgaaggta
     3121 tcgcacacca ttaaaagcat taagccggaa gtcgaatttg gcgttagccc ggcaggcgtg
     3181 tggcgtaacc gatcacacga tccgctcggt tccgataccc gaggcgcggc agcctatgac
     3241 gaatcctacg ctgacacccg tcgatgggtg gaacaaggat tgctggatta cattgctccc
     3301 caaatttact ggccgttctc acggagtgcc gcacgttatg acgttttggc aaaatggtgg
     3361 gcagatgtcg ttaaaccgac caggacccgc ctgtatatcg gtatcgcctt ctataaagtg
     3421 ggtgaacctt caaagataga gccagactgg atgattaacg gcggcgtacc ggaactgaaa
     3481 aaacagctcg atcttaacga tgccgtgcca gaaattagcg gcacaatctt gttccgtgag
     3541 gactatctga ataaaccgca gactcaacaa gcggttagct atctgcaaag tcgttggggc
     3601 agttaaactc ccattcatac tttgtgacaa gccggtggtt tacccaccgg cttgctaccc
     3661 tcttgaaata tcccactagt aatcatgctt acttaagtca aattaactac gcaattaacc
     3721 acacttagtt aaacagtcat aaatccccta ataaaaatgt atgaacatga aaaaacatac
     3781 gatattcgct gtcaccgaaa aataattacg atgggctata ctcttctgaa gaacagtagc
     3841 gtagattgaa actgtcctaa gtcgcttatt tcttttcagt atatcttcat atttcaggag
     3901 aatatggtgc agttagtcga tctcgcacgt tgcgtttcgt tttgattccg tctgctttcc
     3961 cagccttata agggtgtgaa ttatggagat gtattttaaa agaatgaaag atgagtggac
     4021 cgggcttgtc gaacaagcag atccgctcat tcgtgctaaa gccgcggaaa ttgccgttgc
     4081 gcatgcccat tatctgagta ttgagtttta ccgaattgtc cgtatcgacc cgcatgccga
     4141 agaattcttg agtaatgaac aagttgagcg gcagttgaaa agtgcgatgg aacgctggat
     4201 tattaacgtg ctttctgccc aggtggacga tgtcgaaagg ctaatacaaa tccagcatac
     4261 cgttgcggaa gtgcatgccc gcataggaat tccggtagaa attgtcgaga tggggtttcg
     4321 ggtgctgaaa aaaatcctct atccagtcat cttctcttcg gattattccg ccgcagaaaa
     4381 acttcaggtc taccatttct ctattaacag tattgatatc gctatggaag tgatgacccg
     4441 cgcgtttacc tttagtgaca gtagtgcctc aaaggaagat gaaaactatc gtatcttctc
     4501 gttactggaa aacgccgaag aagaaaaaga acggcaaata gcctcaatac tttcatggga
     4561 aatagatatt atctataaag tcctgctgga ttctgattta ggcagtagtt tgcctttaag
     4621 ccaggctgat tttggcctgt ggtttaacca taaaggtcga cattatttta gcggcatagc
     4681 cgaagtgggc catatctccc gtctgattca ggatttcgac ggtattttca atcaaaccat
     4741 gcgtaacacc aggaatttga ataacagaag tttacgggtg aaatttttat tacagataag
     4801 aaataccgta tcgcaaatta ttaccttgtt gcgtgaattg tttgaagaag tatcgcgcca
     4861 cgaagtcggt atggatgtac tgacgaaatt acttaaccgc cgtttcctgc cgacgatctt
     4921 caaacgcgaa attgcccatg ccaaccggac cggtacaccg ctgtcagtgc tgattattga
     4981 cgttgataaa ttcaaagaga tcaacgatac gtggggccat aacactggcg atgaaattct
     5041 gcgtaaagtc tctcaggcct tttatgacaa cgtccgcagt agtgattatg ttttccgcta
     5101 cgggggcgat gaatttatca ttgttttgac tgaagcatct gaaaacgaaa cgttacgtac
     5161 cgcagaacgt attcgcagtc gggtagagaa aaccaaactg aaagccgcga acggcgaaga
     5221 tattgccctc tcactttcca tcggtgccgc catgtttaat ggtcatcctg actatgagcg
     5281 cctcattcaa atagccgatg aagctctgta tatcgccaaa agacgaggta gaaaccgtgt
     5341 tgaactctgg aaagccagtc tttagatgcg ccaggatgca gaggtaatca tgaagctaac
     5401 cgatgcggat aatgccgccg atggcatttt tttccccgcc cttgagcaaa atatgatggg
     5461 cgcggtgtta attaacgaaa atgatgaagt aatgtttttc aaccccgccg cagagaagct
     5521 ctggggatac aaacgtgaag aagtcattgg caataacatt gatatgctga ttccgcggga
     5581 tttgcgtccc gcgcatcctg aatacatccg tcacaaccgt gaaggcggta aagcgcgcgt
     5641 cgaggggatg agtcgggagc tgcagctgga gaaaaaagac ggcagtaaaa tctggacccg
     5701 ttttgcgcta tcgaaagtga gcgccgaggg taaaatttat tacctggcgc tggtacggga
     5761 tgccagcgta gaaatggcgc aaaaagaaca gacccgacaa ttgattattg ccgttgacca
     5821 tctcgaccga ccggtgattg tcctcgatcc ggaacgccat attgtgcagt gcaatcgcgc
     5881 atttaccgaa atgtttggtt actgcattag cgaagccagc ggtatgcagc ccgattcact
     5941 ccttaacatt ccagaattcc ctgctgataa ccgcattcgt ttacaacagt tgctatggaa
     6001 aaccgcccgc gatcaggacg aatttctgct gttgacgcgc accggtgaaa aaatctggat
     6061 taaagcctct atcagcccgg tctatgatgt gctcgcgcat ctgcagaacc tggtaatgac
     6121 tttctcggat atcaccgaag aacggcaaat ccgccagctt gaaggcaata ttctcgccgc
     6181 catgtgcagc agcccgccat ttcatgaaat gggggaaatc atttgtcgta acatcgaatc
     6241 tgtactcaac gaatcgcatg tttcgctgtt cgcgctgcgc aacgggatgc cgatacactg
     6301 ggcgtcatct tcgcacggtg cagaagttca aaatgcgcaa agctggtcag cgaccattcg
     6361 tcagcgtgat ggtgcgccgg cggggatcct gcaaattaaa acctcgtcag gagcagaaac
     6421 cagcgccttt atcgaacgcg tggcagatat cagccagcat atggccgcgc tggcgctgga
     6481 acaggaaaaa agccgtcagc atattgaaca actcatccaa tttgatccga tgaccggtct
     6541 gccaaatcgc aataacctgc acaattacct cgatgacctg gtcgacaaag ccgtctctcc
     6601 ggtggtctat ctcattggtg ttgaccatat tcaggatgtg attgatagcc ttggctatgc
     6661 gtgggccgat caggcattgc tggaagtggt caatcgcttt cgtgaaaaac tcaaaccgga
     6721 tcaatatctc tgtcgtatcg aaggtacgca gtttgtcctc gtgagcctgg aaaacgacgt
     6781 cagtaacatt acccaaatcg ccgatgagct acggaatgtg gtcagcaagc cgataatgat
     6841 tgacgataag ccattcccgc tcaccttgag cattggcatc agctacgacg tgggtaaaaa
     6901 ccgcgattac ctgctctcca ctgctcacaa tgcaatggat tttattcgca aaaatggcgg
     6961 taacggctgg cagttcttca gcccggcgat gaacgaaatg gtaaaagagc gtttggtttt
     7021 aggcgcggcg ctgaaagaag caattagcaa taaccaactg aaactggttt accagccgca
     7081 aatcttcgca gaaacgggtg aactgtacgg catcgaagcc cttgctcgct ggcacgatcc
     7141 cctgcatggt catgtgcccc cttcacggtt tattcctctc gcagaagaga tcggtgaaat
     7201 cgaaaatatt gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag
     7261 ccagaatatt catatcccgg cgctttccgt taacttgtcg gcgctgcact ttcgcagtaa
     7321 tcagctgcct aatcaggtgt ctgatgcaat gcaagcctgg ggtatcaacg gccaccagct
     7381 gacggtggaa atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat
     7441 tcagatcctg cgcgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc
     7501 cggattatcc cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagttttgt
     7561 cgatcgttgt ctgaccgaaa aacgcatcct tgccttactt gaagccatta ccagcattgg
     7621 gcaaagcctt aatttaaccg tcgtggcgga aggcgtcgaa accaaagagc aatttgagat
     7681 gctacgcaag atccactgtc gcgttattca gggatatttc ttttcccgcc ccctacccgc
     7741 cgaagaaatt ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct
     7801 cgcccgcact cgcgggtttt cctttaacgt gacactgtca cttaaatact gtgatttcag
     7861 ctacgattcc gggagattcc ttccttaact tcccctcttg caccaaaact gcactacgct
     7921 gcacaaatgt tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc
     7981 ccggcatggc atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa
     8041 ccgtttatgt cggataccac cgtactggtt gatttagccg tgatcttccc tgatctggag
     8101 atcgaactga aatacgcctg cgctgataac atcacaggca aagctattta tcagcaagcg
     8161 cgttgtctgt tacacaagga tgcgattact gcgctggcga aaagtatcag tatcgcccag
     8221 ctgtcaggct tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg
     8281 ttgtggcaag cctgcccaga cccgcaatat gttgttgatg tggcggtcgg ttctaatcac
     8341 agccgtggca cggcgatcga cctgacgctt cgtgatgagc acgggaacat cctggatatg
     8401 ggggcagagt tcgatgaaat gcacgaacgt tcccatgctt atcacccttc cgtcccgcca
     8461 gccgctcagc gcaatcgcct gttgctgaat gcgattatga ctggtggcgg ttttgtcggt
     8521 atcccgagcg aatggtggca cttcgaatta ccccaggcag cgagttaccc acttctcgct
     8581 gatcaattca cctgttttat atcccctggc acacagcacg ccagttaatc aggaaactgt
     8641 catgaagaga tcgatttcgt ttcgtcctac attgctcgcg atcgtccttg ccacaacaat
     8701 gccggttgcg cacgccgccg taccgaaaga tatgctggtg atcggtaaag ctgccgaccc
     8761 acaaaccctc gacccagcgg tgacaattga taataacgac tggacagtga cctacccgtc
     8821 ttatcagcga ctggttcagt acaaaacgga cggtgataaa ggctcaaccg acgttgaagg
     8881 cgatctggcg agtagctgga aagcgtctga cgatcaaaaa gagtggacgt tcaccctgaa
     8941 ggacaatgct aaatttgccg atggcacacc tgtcactgcc gaagcagtaa aactctcttt
     9001 tgagcggtta ctaaaaatcg gccaggggcc agcagaagca tttcccaaag atttaaagat
     9061 tgatgctccc gacgaacata cagtgaggtt tacccttagc cagccattcg caccgttcct
     9121 ctacacgctg gcgaatgacg gtgcatccat tatcaatccg gcggtcttaa aggagcatgc
     9181 ggcggatgat gcccgtggtt tcctcgcgca aaataccgct ggctccggac cgtttatgct
     9241 gaaaagctgg caaaaaggtc agcaattagt tctggtgcca aatccgcatt accccggcaa
     9301 taaaccgaat ttcaaacggg tatcggtaaa aattatcggt gaaagtgcct cccgtcgcct
     9361 gcaactctct cgtggcgaca ttgacattgc cgatgcgctg ccggtggatc aactcaacgc
     9421 actgaagcag gaaaacaaag tcaatgtggc agagtatccg tcactgcgcg tcacctatct
     9481 gtatctgaat aacagcaaag cgccacttaa tcaggcggat ctgcgtcggg ccatttcctg
     9541 gtctaccgat tatcagggta tggttaacgg cattctgagt ggtaacggaa aacaaatgcg
     9601 cggcccgatt ccggaaggca tgtggggata cgatgcgacg gcaatgcaat acaaccatga
     9661 cgaaacgaaa gccaaagccg aatgggataa agtgacgagc aaacccacca gcctgacgtt
     9721 tctctattct gataatgatc cgaactggga gcctattgct ctggcgacac aatccagtct
     9781 caacaagctg ggcatcaacg tgaagctgga aaagctggcg aacgccacca tgcgcgacag
     9841 agtgggtaaa ggtgattacg acattgcgat tggcaactgg agcccggatt ttgccgaccc
     9901 gtatatgttt atgaattact ggtttgagtc cgacaaaaaa ggtctgccgg gtaaccgctc
     9961 gttctatgaa aacagtgagg tcgataagct actgcgcaat gcgctagcga ccaccgacca
    10021 gacgcagcgt acccgggact accagcaggc acagaaaatc gtcattgatg acgctgctta
    10081 tgtgtacctg ttccagaaaa actaccaact ggcgatgaac aaagaggtga aaggctttgt
    10141 gttcaatccc atgctggaac aggtcttcaa tatcaatacc atgagtaaat aacgtgacct
    10201 tctggagtat tttacgccaa cgctgctggg ggctggtgct cgtggtggcg ggcgtctgtg
    10261 tgataacgtt tattatctcg cacctgatcc ccggcgatcc ggcgcggtta ctggcgggtg
    10321 accgcgccag cgatgctatc gtggataata ttcgccagca actgggactg gaccagccac
    10381 tgtacgtaca gttttaccgc tacgtcagcg acctgtttca gggtgacctg ggaacatcca
    10441 ttcgtaccgg gcgtccggtg ctggaagagt tgcgcatatt tttcccggcg acgctggaac
    10501 tggctttttg tgccctgctg ctggcactcc tgattggcat cccgctgggc atactctctg
    10561 cagtctggcg aaatcgctgg ctggatcatc tggtgcgaat aatggccatt accggaatct
    10621 ccacacctgc gttctggctt gggctgggcg tcattgtgct gttttatggt catctgcaaa
    10681 ttcttcccgg cggcggaagg cttgatgact ggctggatcc acctacgcac gttaccggct
    10741 tttatctgct cgatgcgctg cttgaaggca acggtgaagt cttcttcaat gcgctgcaac
    10801 atctcatctt accggcatta acgctggcgt tcgttcacct gggaattgtc gctcgccaga
    10861 tccgctcagc tatgctggaa caattgagtg aagattacat tcgtaccgcc cgggccagcg
    10921 gcttgcccgg ctggtatatc gttttatgtt atgcgctacc caatgcgttg atcccctcga
    10981 ttaccgtatt ggggctggcg ctgggcgatt tgttgtatgg cgcagtgctc accgaaaccg
    11041 tttttgcctg gcccggaatg ggtgcatggg tagtaacatc aatacaggcg ctcgacttcc
    11101 cggcagtgat gggctttgcc gtcgtggttt cgtttgctta tgtgctggtg aacctggtgg
    11161 tggatttgct ctatttgtgg attgatccgc gtatcggacg tggaggtggt gaatgatgct
    11221 aagcgaggaa acgtcagccg tgcgcccacc aaaacaaacg cgatttaacg gtgcaaaact
    11281 ggtatggatg ctgaaaggca gcccgcttac cgtgaccggc gcagtcatca ttgtattaat
    11341 gctactgatg atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt
    11401 aaccgcccgc cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg
    11461 cgatctgttt agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt
    11521 ggtcgccatt gcaggtatga tggggtcgct actcgggtgt ctatccggtg tgcttggcgg
    11581 acgcgcagac gcgattatca tgcgcatcat ggacattatg ctgtcgattc cttcgctggt
    11641 gctgacaatg gcactggcag ccgctctcgg gccgagtttg tttaacgcca tgctggcgat
    11701 tgctattgtg cgaattccct tttatgtgcg cctggcgcgg gggcaaacat tagttgttcg
    11761 ccagtatacc tatgttcagg cggcgaaaac ctttggtgcg tcgcgttggc atctgatcaa
    11821 ctggcatatt ttacgtaact ccctgccgcc gctgattgtg caggcatcgc tggatatcgg
    11881 tagcgcgatt ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag
    11941 tgctgaatgg ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta
    12001 ttgcgcattt ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga
    12061 tggtattcgc gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt
    12121 ctggacattc aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc
    12181 aacaatgtgt ccttgcagat taaccgcggt gaaattgtcg gtctggtggg agaatccggc
    12241 tcaggtaaat cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc
    12301 gtacatcggg ggcagatttc actgctggga gacgatgttc ttaacgctcg ggaaaaacag
    12361 cttcgtcagt ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt
    12421 aatccgacac gtcgaatagg tcttcagatg atggacgtga tccgccatca tcaaccaata
    12481 agtcgtcggg aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat
    12541 gccgtggaag ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta
    12601 atgattgcgc tggcattctc ctgcgagccg caattgatta ttgccgacga accgactacg
    12661 gcgctggacg tcacggtaca attgcaggta ctgcgtctgc ttaaacataa agcccgcgcc
    12721 agtggaactg cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat
    12781 agcgtttacg tgatgtatgc cggaagcgta attgaaagcg gcgtgacggc agacgttatc
    12841 catcatcccc ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta
    12901 ccacgccagc cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac
    12961 ggctgcgctt ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg
    13021 ctaacagcgt gtggtgacaa caaccaccgc tgcgcctgtt ggtatcctca gcaggaggtc
    13081 attagtgtct gacacgttat taacgttacg cgacgtccat atcaatttcc ctgcccgaaa
    13141 aaactggctt ggtaaaacta cggaacatgt tcatgccatc aatggtattg atttacagat
    13201 ccgtcgtggt gaaaccttag ggatcgtcgg tgagtcaggc tgcggcaaaa gcaccctcgc
    13261 acagctttta atgggtatgc tgcaacctag ccacgggcag tacatccgtt caggctcaca
    13321 acgcattatg cagatggtgt ttcaggaccc gctctcttcg ctcaatccgc gcttaccggt
    13381 gtggcgcatc atcacagaac cgctctggat agctaagcgt agtagtgaac aacagcggcg
    13441 agcgttggca gaggagctgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgctt
    13501 gcctcatgcg ttctcaggcg ggcagcggca acgtatcgcc attgccagag cactctcttc
    13561 gcagcctgac gtgattgtgc ttgatgaacc aacctcggcg ctggatatct ccgtgcaggc
    13621 gcagatcctc aatttactgg taacgctaca ggaaaatcgc gagctgactt atgtgctgat
    13681 ttcacacaat gtttcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg
    13741 acagattgtg gaactaggag acgtgcaaca ggtgctgaca gctccggcac atccatacac
    13801 ccgattattg ctggattccc tccccgccat taataaaccg ctggaggaag aatgggcatt
    13861 acgtaaaacg gatctaccag gaaaccgcac gttgccacaa ggctgttttt tccgcgaacg
    13921 ctgccctctg gcaacccacg gatgtgaagt ccggcaatca ttaacaataa gggaggacgg
    13981 acgtgagatc cggtgctggc gggcgctgta gagacgctaa caacgcatca ggcaatcaca
    14041 cgcctgacgc gtcatccggc aacgctttac gatttcaact ggtaatcaag cgtaatttca
    14101 gctttcagta cctgagaaac cgggcatcct gcttttgctt tctggataat gccgtcaaaa
    14161 gtagaggcat caatacccgg caccgcaact tcgctcttca gtgcgatttt agtaatcgca
    14221 aaacctgcat ccactttatc cagcgacaca tcggcggtgg tatcaatcga tgttggcgtg
    14281 aatcccgctt cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg
    14341 ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac
    14401 ggctgttggt tcagcacgcc gctctcggtg gatacagttc ccttcccgcg tttgatatcg
    14461 ccttcccagt gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa
    14521 aaaggaaatt atagactacg tgagaaaccg cacgaggata agcaggatat tccgaataaa
    14581 atccggcgaa aacat
//
LOCUS       DAOTRK010000120        14271 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.120, whole genome shotgun sequence.
ACCESSION   DAOTRK010000120 DAOTRK010000000
VERSION     DAOTRK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.120"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(236..1201)
                     /gene="alx"
                     /locus_tag="QIE41_003517"
     CDS             complement(236..1201)
                     /gene="alx"
                     /locus_tag="QIE41_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDS3532362.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(1485..2471)
                     /locus_tag="QIE41_003518"
     CDS             complement(1485..2471)
                     /locus_tag="QIE41_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDS3532363.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(2550..3242)
                     /locus_tag="QIE41_003519"
     CDS             complement(2550..3242)
                     /locus_tag="QIE41_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDS3532364.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(3319..3822)
                     /locus_tag="QIE41_003520"
     CDS             complement(3319..3822)
                     /locus_tag="QIE41_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDS3532365.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEQQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            3907..5043
                     /gene="rlmG"
                     /locus_tag="QIE41_003521"
     CDS             3907..5043
                     /gene="rlmG"
                     /locus_tag="QIE41_003521"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDS3532366.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            complement(5161..7179)
                     /gene="fadH"
                     /locus_tag="QIE41_003522"
     CDS             complement(5161..7179)
                     /gene="fadH"
                     /locus_tag="QIE41_003522"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDS3532367.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYPNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSKNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(7484..9025)
                     /locus_tag="QIE41_003523"
     CDS             complement(7484..9025)
                     /locus_tag="QIE41_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532368.1"
                     /translation="MVTPCHIKASAGMTLPLALENQESCRTRLEEMINLINRNTDCST
                     GVPDQVNALVATMADALQPYTVMTNELPEYPNMPERVLYIPEPFSFCFSGHTITFTNT
                     KRDYNGDNLVNIAVSKDGGNARGDADCALFHAFVTKMMSRSRQPGTYDFFPSLKEEKQ
                     GEGMHFMVTPRNSSISAGAIMSRPLLQTQHGCEILNKIINLINADTDEVNELVVAMTN
                     ALFLYQTVVRTELQRTEPFTEPFRFSFFFSSHTITFTKAESNSKLVQIEVSRGGVTVR
                     ECVGSARFRTIAAAMVSCRISEAAATSLALQQTLHSRVILKLMIKLINADTDRSIAVP
                     DRVNELAVAMTNALYRYQTVRGDKQIIPESFSFGFSGHTITVTPGRYSRSVNLKVCGG
                     GVPACATADRTLFRTIAAALVSSNQQGTLDFRSSLPFLVERNLSTENEATPPLPTRPR
                     VQVRRKVAKRTSPHVKNIRSFRSSQPLLMDGNQSAEHAPGPQLPSVLARQKGTKKPPL
                     STKSF"
     gene            complement(9317..11668)
                     /gene="ygjK"
                     /locus_tag="QIE41_003524"
     CDS             complement(9317..11668)
                     /gene="ygjK"
                     /locus_tag="QIE41_003524"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDS3532369.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNAINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     SKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLPNGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(11685..12755)
                     /gene="ygjJ"
                     /locus_tag="QIE41_003525"
     CDS             complement(11685..12755)
                     /gene="ygjJ"
                     /locus_tag="QIE41_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDS3532370.1"
                     /translation="MKLITAPCRALLALPFCYAFSAVAEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALQVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GIMASAMYEALKGVYLSTAYTYAKHRHDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            complement(12889..>14271)
                     /locus_tag="QIE41_003526"
     CDS             complement(12889..>14271)
                     /locus_tag="QIE41_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDS3532371.1"
                     /translation="TFAAVYSFNNVINNNIELGLASAPMFFLATIFYFIPFCLIIAEF
                     VSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTSLLPRVIAYASYAFLGYE
                     YIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLMLLLTLSYILLAGTALVG
                     GVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIIL
                     AGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSF
                     TAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIP
                     MLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIV
                     VSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERH
                     IEATPASNVD"
BASE COUNT         3369 a         3855 c         3701 g         3346 t
ORIGIN      
        1 ccctcagctt atcccttgcg gctaccgggt cgggcgcaag ggaacgtccc tgtaagatgc
       61 gccctcgccg cgcatccatg cgcgtcgccc cggcctccag ggaacgcttc ggcgattttg
      121 acgccaccaa cacccgtgcg gtctttttgt aaagagctat aagccgatta cataattaat
      181 agctcacctt tgtctggaat gagttatctc taggaatggc ttaatataca gcgaatcatt
      241 ctccccgctg cttatcatgc cgataattca cccaggcgtt gataataaat gtcatcacca
      301 gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca
      361 gcatcttgat accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac
      421 gttctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatc gcaaacaggt
      481 ttgaggtcag cacaataaac ggatcggtgg tcacggcgaa gatagccggg atgctatcca
      541 cggcgaaaat cacgtcgctc aattccacca gaatcagcac cagcatcagc ggcgtggcat
      601 acagcaagcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca
      661 aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg
      721 cgagcgccat cttcacgccg gtaaacaaca ggaaggcacc gaagatatac aggatccagt
      781 cgaactgcga aatcagccag ctgccagtga agatcatgat ggtacgcaga acaatcgccc
      841 cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc cggaacagag aaatagctga
      901 acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacccg
      961 tgagaaaggc cagtgcctgt ggatcggcaa cggcgcgacc ttcggtttgc accagatacc
     1021 accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag
     1081 cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca
     1141 gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca
     1201 tagaagttcc ttacacataa aaaaaacggc caacgtcgga agacgtcagc cgctgctttt
     1261 tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga atacgtctgc
     1321 gtattccttt cgggttgccc ggtgaccgga tgtggttttt cacacatcgt aatgacgatc
     1381 gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc attcagtcag
     1441 tcaatggtct gttacatgcc ttttacactg ctttactttg gcaattatag ttttacgcta
     1501 tctgccggaa aaatcacccc agtctggcgg cggatctcgg tcagcagttt ggcggtgatg
     1561 cgactgaccg ccagcccagg atgatccacc agatgttcat ccaccagttc agcaaacagc
     1621 tctgcttcgt agagcatggt attaatatgc tgcggttggg tgagatcctg catctggctg
     1681 ccacgcggaa caaagcaaac tttctggcat tcagacagtt tttcaatcac cagcgatcct
     1741 gcttcgccct gaatctcgct tgccaggaca gaatcgctca cttttgagtg ctgcaacgta
     1801 acgctgaaat caccgtaatc catcaccacc acgccgtggg catccacacc gcttgccagc
     1861 aaactggcgg ttgcctgcac gcttttcggt tcaccgaata atgccaccgc cgacgccagg
     1921 cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa ggtgttggga
     1981 ttctcaccat ccaggtaacg ttgataccgc gaggaatact ggcaatagtt gaaaaagact
     2041 ttacgcagtt tgccgacttt cggcagtgcc tggcgtaaca gatgaaagtt cggcaggctg
     2101 gcggttttaa atgcctcaaa cagcaccacc tgattttccc gcgcacaggc aatggcggca
     2161 tccacttccg ccagattcga tgccagcggc ttctcgcaaa tcacatgaat tttgtggcta
     2221 aggaaaagtt gtgtctggga aaagtgcagg gcattcgggc tggcaatata caccgcgtca
     2281 atggcatcgc tttctgccat cgcttccagc gaggtaaaca gatgctcgac agaaaaatca
     2341 ttggcgaagt gctgggcctg ctcaaggctg cgggaataaa cggcggttaa cttgtattta
     2401 ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc aatcacagcg
     2461 aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg tagcacgcct
     2521 ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc atgctcacta
     2581 aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag gatttttgcg
     2641 tcaatcaccg cgctgacgcg agcgagtttc tcccgcagtt gagtataaaa accgtgcttc
     2701 atattgaggt ctggcgcgtt aaaaccgata gcgtcgatgc cgtactgttt tgccagccag
     2761 atggcgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc cccaaacacc
     2821 tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca gaagatcact
     2881 ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc atcgtaattt
     2941 ttacgtccgt tatcgccact gactaacagc catttcactt ttccggcgtg gtacaatgct
     3001 gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctggctcc cagcaataaa
     3061 cccacgttac gcgccggaac tgcgttgaca tcgctccagg tcagctgctt gctggtgttg
     3121 accataacgc gatcggcaat aaaaatggtc gctcctgcta taagcaacaa taaacagcca
     3181 atcttaagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa ggcgcgcagc
     3241 atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag gaagaaaaga
     3301 taacgcctga catcattttc agattttgtc ctgcccaagt gacaactgcg ttagccacag
     3361 acgagtgtcg aattcaagct ggtggtactg ctgctccata tgacagcaca attgatagaa
     3421 cgctttgtta tgctctttct ccttcaggtg cgccaactcg tgcaccacga tcatgcgcaa
     3481 aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctccgcct ttgctttcag
     3541 cttgccgccc tgcacacgcg aaacagcggt atgtaacccg agcgcgtttt tcagcacatg
     3601 gatcttgttg tcatacatca ctttagtgat cggcggggca ttgcgcagaa actgattttt
     3661 cagatcctgg gtatattgcc agagggcttt atcggtggcg tagtcgtgtg ttcccggata
     3721 acgttttgcc agcacatcgc ccagacgctg ttcgttaatc agcgttcgca cctgggaaag
     3781 tagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga
     3841 gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta tcatccagga
     3901 gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg
     3961 acggatgacg ttaacccgct acaggcgtgg gaagcggcgg atgaatattt gctgcaacag
     4021 ttggacgaca cagaaatccg cggcccggtg ttgatcctga atgatgcctt tggtgcgtta
     4081 agttgtgcgc tggcggaaca taagccatac agcattggcg actcatacat cagcgaactg
     4141 gcgacgcgcg agaatttacg cctcaacggg attgatgaat cgagcgtgaa gtttctcgac
     4201 agcaccgctg actacccgca acagccggga gtggtgctga tcaaagtacc gaaaacactg
     4261 gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgccaga tacgcgtatt
     4321 attgccggtg ccaaagcccg tgacatccac acttccacgc tggaactgtt cgaaaaagtg
     4381 ctcggcccga ccaccaccac gctggcatgg aagaaggcac gcttaattaa ctgtactttc
     4441 aacgagccgc cgctggccga tgcgccgcag accgttagct ggaaactgga aggtactgac
     4501 tggactatcc acaaccatgc gaatgtcttc tcccgcaccg ggctggatat tggcgcgcgc
     4561 ttctttatgc agcatctgcc agagaatctc gaaggtgaga ttgtcgatct cggttgtggt
     4621 aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc
     4681 gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgcccgag
     4741 gcattggatc gctgcgaatt tatgatcaac aacgcgctct ccggcgttga gcctttccgc
     4801 tttaatgctg tgctctgcaa cccgccgttt catcagcaac atgcgctgac cgataacgtc
     4861 gcctgggaga tgttccacca cgcccgccgc tgtctgaaaa tcaacggcga gctgtatatc
     4921 gttgccaacc gtcacctgga ttacttccat aaactgaaga agattttcgg caactgcacc
     4981 accatcgcca cgaataataa atttgtggtg ctgaaagcag tgaagctggg gcgtcgtcgg
     5041 taagattggc agctacctgc cagataaggc gtaaacgcct tatctggcat acgttcagca
     5101 cattaccccc aggtcggata agacgcggca agcgtcgcat ccggcacttc aatgcacgat
     5161 ttaaatctcc agcgccagcc gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat
     5221 agctacatcg cagccgccaa ttaaatgcac agttttcccg ctatcaatca gcggttgcgc
     5281 cagcgcgcgg tttggctctt gccctgcgca gatcaccaca ttgtccactg ctaatacctg
     5341 cgtttcgccg ttgatcacca catgcagccc gtcatcgtcg atcttctgat aacttacgcc
     5401 tgggatcatt ttcacgccgc gcgagagcag ggtggtgcga tggatccagc cggtggtttt
     5461 gcctaacccc tgtcccggtt tgctggcttt gcgctggagc atcacaatct gccgtgggct
     5521 acgcgggatc tgcattccct gcgggcttaa gccaccagcc tgttgcaggc tgctgtcgat
     5581 cccccactca ttacagaact cggcaatgtt cttgctggtg gactcacccg gctgacttaa
     5641 aaacatcgcc gtatcaaaac caatcccgcc acaaccgatg attgctacct ttttgcccac
     5701 aggcgctttg tcgcgcagta cgtcgagata actcaacacc ttcggatgat cgattccgtc
     5761 gataggtgga atgcgcggta cgatcccgct agcgaggatc gtttcatcga acgactgtaa
     5821 ctgatccgcc gtcacggtgt gattgagttt cagcgtcacg cctgttactt cgatcatccg
     5881 gcggtaatag cgcagcgttt cgtaaaactc ctctttgcca gggatctgtt tggcgatatt
     5941 aaactgaccg ccgatctcac tgtgagcatc aaacaacgtt acctgatgac cacgtgccgc
     6001 cgcgttaatg gcaaacgcca gcccagccgg accagcgccg accaccgcca gatttttttt
     6061 ctgcacggcg ggaaggatcg gcattttggt ttcgtggcag gcgcgaggat tcaccaggca
     6121 cgaggtgact ttgcccacga agatctgatc gagacaggcc tggttgcagc caatacaggt
     6181 gttgatctca tcggctcgcc ccgattgcgc ttttgacagc agctccgcat cagcaagaaa
     6241 cggtcgtgcc atcgatacca tatcggcatc accgcgcgag agaatgtcgt cggcaacctg
     6301 cggatcgtta atccggttgg tggtcaccag cggcagcgag acgtggcctt tcagtttgcg
     6361 cgtgacccag ctaaaggcac cgcgcggcac gggcgtggca atggtcggaa tacgtgcttc
     6421 atgccagcca atgccggtgt tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag
     6481 ccctaccgtt tcggcaaaag taccgccgcc ttctaccagg tcgagcatcg acagacggta
     6541 gataataatg aagtcattgc cgacgcgttc gcgcactgct cgcaccactt caactgcaaa
     6601 gcgcatccgg ttagggtaat cgccgcccca ctggtcgcta cgctggttag tgcgcagggt
     6661 cagaaactcg ttgatcaaat acccttcgga acccatcact tctacgccat cgtatccggc
     6721 ctcccgcgcc agttgcgcgc agcgggcgaa attgtcgatc agttgcagga tctcttcatg
     6781 gcttaactca tggggaacga aacggttgat gggagcctgc aatgcggaag gagcgaccag
     6841 atgcggttgg tagctgtagc gcccggtatg caaaatttgc agggcgattt tgccgccttc
     6901 ctgatgtacg gcttcggtta tggtgcgatg gtgcgggatc tggcttgcgt cattgagcat
     6961 tgcgccgcct tccatgccaa cgcctgttaa atctggtgca ataccgccgc tgacaatcaa
     7021 cgccacgcca tgacgggcgc gttccgcgta aaacgctgcc agccgttcag cgccgtccgg
     7081 gtattcctcc agtcccgtgt gcattgaacc catcagtact cggtttttta acgtggtgaa
     7141 gcctaaatcc agcggggcga acagcgacgg gtagctcata aaattgtcca gtatgtaaaa
     7201 taattgttat gtggtcggat gagttctaat ttagccgtcg ccagagaaaa gggaaaaagg
     7261 gagtgcgggg tttgtgatgg gattcaaaaa gaatacgccc attggaaatg ccggatacga
     7321 cgcttgtcgc gtcttatctg tatatggaca cctctcgttg tgcaagtacg gtgttcgaaa
     7381 aattaatagg taacgtggtg gtcgtcctga caggacggca ggctacggag atagtcaatc
     7441 acgctatctt tagcccgctc atccgggggc atcttcgacc atttcagaat gacttcgtcg
     7501 ataatggggg ctttttggtc cctttctgtc ttgccaggac ggagggcaat tgcgggccag
     7561 gcgcatgttc cgcactctga tttccatcca tcagtaaggg ctgtgaagac ctgaaacttc
     7621 ggatgttttt gacatggggg ctggtccttt tggccacttt ccgcctaacc tggacgcggg
     7681 ggcgggtggg cagtggcggg gtagcctcat tttccgtgct caggtttctt tccaccagga
     7741 aggggaggga ggatctgaaa tcaagtgttc cttgctggtt gcttgatacc agtgctgcgg
     7801 caatggtgcg aaacagagta cggtcagctg ttgcacaagc tggcaccccg cccccacaga
     7861 ccttaaggtt cacagagcga ctataccttc ccggcgtgac ggtgatggta tggccggaaa
     7921 acccgaaact gaacgattca gggattatct gcttatcacc cctcaccgtc tggtaccgat
     7981 atagtgcgtt cgtcatggca actgccagtt cgttaacccg gtcaggaaca gctatgctgc
     8041 ggtccgtatc cgcgttgatt aattttatca tcagcttcag aattacccga ctgtgcaggg
     8101 tctgctgcag ggccagggaa gttgcagcgg cctcactgat acggcatgac accattgccg
     8161 cggcaatggt gcgaaaccgg gcactgccaa cacattcacg gaccgtcacc ccgcccctac
     8221 tgacctcaat ctgcactaat ttgctattac tctccgcctt cgtaaaggtg atggtgtggc
     8281 tggaaaaaaa gaaactgaac ctgaacggct cagtgaatgg ctcagtgcgt tgcagctcag
     8341 tcctcaccac cgtctgatac agaaacagcg cgttcgtcat ggcaactacc agttcattaa
     8401 cctcgtccgt atccgcgttg attaaattta ttatcttatt cagaatttca cagccgtgct
     8461 gggtctgcag caggggccga gacattatcg cgcccgcgct gatgctggag ttgcgtggtg
     8521 ttaccatgaa atgcattcct tccccttgtt tttcttcctt gagtgaggga aagaaatcat
     8581 atgttcctgg ctggcgactg cgtgacatca tttttgtgac aaaggcgtga aacagggcac
     8641 agtcagcatc gccacgggca ttccccccgt ccttgctgac cgcaatgttc acgagattgt
     8701 ccccgttgta atcccttttc gtgttcgtga aggtgatggt gtggccggaa aaacagaaac
     8761 tgaacggctc aggaatgtac aggacacgtt ccggcatgtt cggatactcc ggcagttcgt
     8821 tcgtcatcac tgtgtacggc tgcagagcgt ccgccatggt agccaccagt gcgttaacct
     8881 ggtcaggaac acccgtgctg cagtctgtat tcctgttgat taaatttatc atctcttcca
     8941 gacgtgtcct gcagctctcc tggttctcca gggccagggg caatgtcata cctgcactgg
     9001 ctttgatgtg gcatggtgtt accatgtaat gaattccttt ctctcagggg ggcagatagt
     9061 gccgtgtttg ctcactggcc ccccggagag cggctgaccc gtttgttcaa ctcaacagct
     9121 ccagcaccag tcagtgaaca gggcgcataa ccgtcggacc attaaacgtc agacggcaaa
     9181 tcagtattat tttattaatt tcggtcagtt gcattccggt ttttaccgca gtttaaaaga
     9241 aaagagtgcg tatacccccg atgccgcggg ccagggcgga taaggcgttc acgccgcatc
     9301 cgccagctgt tgcaaattac tgcttacgga aaaaatcgtt atacaacata tacaaatgcg
     9361 cggcgctcca ggagaaattg ggtgcgcctt gctgtgcgcc tgtcagcggg ttgtaatttt
     9421 cctgaattgg tccatcggcg gttaaccctt tggcgtgccg gaagaacgta tccgccagct
     9481 tcagggcatc atcgcgataa ccgtaacgct ccattccttt cagaccaaac cagaactgat
     9541 ccacccatac gcgcccgcgc cagtagatat cagcgccaaa ggccggattg gttaacgccg
     9601 ccgttcccag cggaacaaag gtgttgaact ctttaggatc gagcatcacc ttcaccaccg
     9661 cgtcggcgtt ggcctgcgtt gctgcaccgt taaacagcgg tgaccagcct tctggccctt
     9721 taccgcgctc gacaatcggt ttgcccgcgc agccgttcgg cagcggttta tcttcaatgc
     9781 gcacgtcata gtagaactgg gtagtcgggt cgaacataca ggtgttgatg tagtccgcga
     9841 gctgctgtgc caactggcga tagcgtttgg cctcttccgg cttaccgagt atggttgcca
     9901 tctccgccag ataatggtta tcgctgtaca tatagctggc ctgatccacc gactcctgca
     9961 atagcgagta ccccagcaac gttccgtcct gactgcggtt ttcggcgaat ttcaccgtcc
    10021 agtcgctacg tttgccgcca ttagcgacat atttatccag ctgttctttg tcgataaacc
    10081 caaagacggc ggcgtcatca cgacctgatt cccacgacgc agcaacctgt gccggaattt
    10141 ccagactgtc gtactggcct ttctccacca cacgggcgta gttgttcagc ccagactgcg
    10201 tctcttcttt gtcgcctttt ttcaccgtaa acagcatctc gccgctctcg gtgttgtggg
    10261 ctttgtcgcg ggtcgcgcca tattccggca cgccgttgcc gttgtgatcg cggttacgta
    10321 accaccagtc gtgataggcc accagcttcg ggtacatctc tgccaaccag gctttatcct
    10381 gggtgacgtt gtacacttcc atcaccgacc aggcggcaag gctgggtttg gtattacgtt
    10441 cgttccagtt gccgccatcg ccgccacgct cggggctaag gttccacgct atcaggtcgg
    10501 ggacaaagcc cacatcctgc ggacgcacgc tatcgccggg ctggatctgc caggagaaga
    10561 ccgcgcggat attctctttg gcgatgtccg gattgaaatg cgccatcgca aacgcctgct
    10621 tccaggtatc ccacggccag gtctgattgc cggagaacca gcgcccggtc accgacggcg
    10681 tgacggtgtt atatttcacc gcaccgcccg gtgagcgcca gttaccgttg agcgtttcga
    10741 tcgctttcac cgcgacgcgc gtctgttccg gcgtcgcatc cggattggtt aaccctttct
    10801 tcagatactc ttcccagcgt tgctgcgagg cggtgagata aaacgccgga cgcgccagaa
    10861 tatcgcggat ctgcatttgc tctttgctaa cttcctgagc ggtcagcaga tgggaatagg
    10921 tggtgtagag cgtcgtcgaa ccgttgatat gcgccttact ggtaaagcga ttaccgttga
    10981 tttcagtctg cactggcagg gatttatgca cctgatattc tgattcaccg gaggtcagca
    11041 gatcccaggt ggcgcgcact ttgccaaagg tgactttcag gccatcacgg gtggcgctga
    11101 ttttgcgctg atagtcaggg tactcgccag caatggtttt atcggaaagc ggtttccctt
    11161 ctttcgcttc cagtttttcc agcagctcgc cgtcccacac cagatccagc ggtttattgc
    11221 tgatgatttt agtttccagt agtgacgtgc gcggcgtggc gaagcgcaga gtcatttcaa
    11281 cctgcacatc ttttgctgtc agtttttgca ccagcgcgcc gggaatactg tatgcctcca
    11341 gcgtgaagtc gactttctta ctgtcctgcc agacggtcag gcggtcgaaa ttgctggcca
    11401 taaagttgat gtactcttcc gtcagcagcg caacgcccgg aaagccgccc atggtgttcg
    11461 ggccgtctgg caacagatga ccatgccagg ctccgagatc gaaaaacgga ttaaaacgct
    11521 ggtgatcgtc gtaatcgtaa tccttcatgt actgcggcgc gccagtacgg ttaatcgcgt
    11581 ttttataatt gtcggcgtta gcggcatggg cggaaaaact tatcagcaga gcgcaggtta
    11641 ctggcgttaa aattgttttg attttcatga aattacagtc cttattacca gtagaaatat
    11701 tgcatcagga agagttggtc gctgggatcg tgagaggcat ttttcatgta gaagcggcta
    11761 tcaaaagccg ttgcgaaacg tccgccgccg tattcgtacc agataccaaa ctgcatgaaa
    11821 gaggtggttt catcgtcagc gtggtcatgg cgatgtttgg cgtaggtgta agcggtggag
    11881 agataaacgc cttttaacgc ttcatacatg gcgctcgcca ttatcccgct ttcacttccc
    11941 ggtacgtcca gcccgtcggc gcgtccggta tggtgccagg cgcgagcggc aaagttaggg
    12001 gtaagcagtc ccgtcagata gagttgccct gtgccgtcgg tgatttccag accgttcatc
    12061 caggtgatgc cttcggcaag gtcatactgg atgtaaccgt tcatcattgc cggataggtg
    12121 tatttgtcgt cgtagcgatc gtacttgcca aaatgtagcc aggctttgct ttcgtcatga
    12181 tgtccggcag gcgtggcggt gacgctataa cgaaagttgc cgcttaagtt ctgcacctgc
    12241 agcgcggtca taatgtcgcg ggtattggga atgacgtagc ccagatcggg ggtgaagtcg
    12301 ccccaccatt gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg
    12361 ccatcattgg ttcgataacc gccatagaaa cggttaatcc caccttcgaa accaccccag
    12421 ctatggtcag gcacccaggc attaccgtcg tggtcggcct gtacggtcca gccttcgccg
    12481 taaatcaggc cgaaccagga gccgtacttc atctccagtc caccttcaat ataggtgccg
    12541 tcgctgtatt tatgtttatt ctcaccctct aaatccatat atccaccaac ccctaattcg
    12601 ccgtaaaaac gaaatgaagg agaagatgtc gaggtaatgg cgggtgtttt tgtgtcgtca
    12661 tgttctgccg gacgagcttc ttcagcgacc gcagaaaacg cgtagcaaaa cggcagagca
    12721 agtaatgctc tgcatggtgc agtgattaat ttcattgtgt taatccatga gctaaagaaa
    12781 aagtacagcc ttttatcgtt gtaattataa agatataaag gcagaaacta ttttccctct
    12841 ccccatccag gaagagggaa aatgcctgct cattttttat tagtctgctt aatcaacatt
    12901 gctggctggc gtcgcttcaa tatggcgctc ttcagccgtt aatcccttta tatatttact
    12961 gtatttccac cacgcaaagc cgaggaagat aacaataccg ccgacgttat aaaaaatgat
    13021 ggtcagaata ttcgcgccag tcgggaatgt cgaagcgaca aaccctacgg caaatatcgc
    13081 aatcagcatt gaaacaacga taataccggt gcggcgggaa cccatacgga aatcacgtgg
    13141 caggtgatct aatttggcgc gtaaattcag gtaagccagc atgatgaata acggcggaag
    13201 catggacgct gcggcagtca tattaataat agtattcatc agatcctgca cggtattgga
    13261 accgagcatc gggataatca tcagcgggat gacgatcagg aactggatcc acgctgcgcg
    13321 cgccggaacg ccgttttcgt tcagttcgac ggttttctta ccaaagatgc cttccgggat
    13381 ttcggagaag aaaattttca ccggcgttgc ggtccacatc agcagggaac cgaacatcgc
    13441 ggtaaaggac accagaccga caaagcgatt catcaacgct tccggtagac caaaatacgc
    13501 cgccatgccg tggaatacct gcaccgatcc gccagtaaat ttcaactctt tgctgctgac
    13561 gaagacgtta atcagtaccg aggagacgga atacagcaca ccgataaaaa tcccggcgag
    13621 gatgatcact ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acaccgcgac
    13681 ggactccgcg ccacctgcgg ccataaagat ccaggtggta acgccgagga acgcccagtt
    13741 gaagttcggg atcatcgcgt caacagtgat cggatcggca ggctgtacgc cgccaaccag
    13801 cgccgtgcct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
    13861 ggaggtaatt ggccccaaca ttttcgcccc gttggtagaa acccaggtgg agaaggcgaa
    13921 cagcaccata ctgataatgg tggtggcaac cggtgtcata atatattcgt agccgaggaa
    13981 ggcatacgaa gcataagcaa taacgcgcgg taacagcgag gtgaaaaaga acaggtttac
    14041 gaaccagtag gtataggcag taataaatgc ccaacgcccg cccagcgaac tttttaccca
    14101 cgcgtagaca ccagcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
    14161 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
    14221 ctcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag t
//
LOCUS       DAOTRK010000121        13882 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.122, whole genome shotgun sequence.
ACCESSION   DAOTRK010000121 DAOTRK010000000
VERSION     DAOTRK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.122"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(4..708)
                     /locus_tag="QIE41_003527"
     CDS             complement(4..708)
                     /locus_tag="QIE41_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDS3532372.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(845..1297)
                     /gene="moaE"
                     /locus_tag="QIE41_003528"
     CDS             complement(845..1297)
                     /gene="moaE"
                     /locus_tag="QIE41_003528"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDS3532373.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1299..1544)
                     /gene="moaD"
                     /locus_tag="QIE41_003529"
     CDS             complement(1299..1544)
                     /gene="moaD"
                     /locus_tag="QIE41_003529"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDS3532374.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(1537..2022)
                     /gene="moaC"
                     /locus_tag="QIE41_003530"
     CDS             complement(1537..2022)
                     /gene="moaC"
                     /locus_tag="QIE41_003530"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDS3532375.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2025..2537)
                     /gene="moaB"
                     /locus_tag="QIE41_003531"
     CDS             complement(2025..2537)
                     /gene="moaB"
                     /locus_tag="QIE41_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDS3532376.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2559..3548)
                     /gene="moaA"
                     /locus_tag="QIE41_003532"
     CDS             complement(2559..3548)
                     /gene="moaA"
                     /locus_tag="QIE41_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDS3532377.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDAHQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3536..3680)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3945..4853
                     /gene="yvcK"
                     /locus_tag="QIE41_003533"
     CDS             3945..4853
                     /gene="yvcK"
                     /locus_tag="QIE41_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDS3532378.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLNEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5006..7027)
                     /gene="uvrB"
                     /locus_tag="QIE41_003534"
     CDS             complement(5006..7027)
                     /gene="uvrB"
                     /locus_tag="QIE41_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDS3532379.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7606..8283)
                     /gene="bioD"
                     /locus_tag="QIE41_003535"
     CDS             complement(7606..8283)
                     /gene="bioD"
                     /locus_tag="QIE41_003535"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDS3532380.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8276..9031)
                     /gene="bioC"
                     /locus_tag="QIE41_003536"
     CDS             complement(8276..9031)
                     /gene="bioC"
                     /locus_tag="QIE41_003536"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDS3532381.1"
                     /translation="MTTVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            complement(9018..10172)
                     /gene="bioF"
                     /locus_tag="QIE41_003537"
     CDS             complement(9018..10172)
                     /gene="bioF"
                     /locus_tag="QIE41_003537"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDS3532382.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKLSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDSLLEVLHDNG"
     gene            complement(10169..11209)
                     /gene="bioB"
                     /locus_tag="QIE41_003538"
     CDS             complement(10169..11209)
                     /gene="bioB"
                     /locus_tag="QIE41_003538"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDS3532383.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11296..12585
                     /gene="bioA"
                     /locus_tag="QIE41_003539"
     CDS             11296..12585
                     /gene="bioA"
                     /locus_tag="QIE41_003539"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDS3532384.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRLDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12644..13120
                     /locus_tag="QIE41_003540"
     CDS             12644..13120
                     /locus_tag="QIE41_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDS3532385.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLARASITAMFS"
BASE COUNT         3240 a         3748 c         3530 g         3364 t
ORIGIN      
        1 gaattaacgg cggttgccga agatccgcaa caacatcagg aacaagttga tgaagtccag
       61 atacaaggtt aacgcgccaa gaatggaata tttgcgcagg ttcgacgtgt cgcgggtatc
      121 aatctgctca cccatatttt tcaatttctg agtgtcatac gccgtcaatc cgacaaagac
      181 aatcacgccg atgtaggtaa ctgcccacat caatgcttcg cttttcagcc agaagttgac
      241 cagcgatgcc agcacaatgc cgattaacgc cataaacagc atattgccaa agccgcttag
      301 atcgcgcttc gtggtgtaac cgtacaggct cattgcgcca aacatcccgg cagtaacgac
      361 gaaagtactg gcgatagaag cagcggtata gacaatgaaa atactggaaa gcgtaagacc
      421 cgtcagcgcc gaataaagca taaagagcat cgtcgttaca cctgcgctca gcttttgaat
      481 catcgctgat aacacaataa ccaatgctaa ttgcgcgatg atcagaccga ttaaaaagac
      541 acggttagtg aacaacagct ccatcacggc cgcggaatta gccgcatacc aggcaacaaa
      601 tgcggtcagc aacaagccaa cggtcatcca gccatagact tgcgccatat aagtttgcaa
      661 gccagcccgg ggttgtacaa ttgaatcaga acgtgggaat ctgtccatga cgatctcctg
      721 aagatataag gaatatctta aggatactgc aaaatgatga ggctgtgcat cgacgcagcg
      781 taaacgcatg tactgagcgg tgaaattgcc ggacgcagcg gtgccttatc cggctaacaa
      841 aaaactacca gcgttttgcc gcctgctgat cgctctcccg agcttcgacc cagcggtcgc
      901 cttccggcgt ggcttcgcgc ttccagaacg gtgcgcgggt tttgagataa tccataataa
      961 actgcccggc ttcaaacgca ctgctgcgat gcgcactggt gacaccgaca aaaacgattt
     1021 catcgcccgg ccataattcc ccgatgcggt gaatcaccgt gacgcgcccc agcggccagc
     1081 ggttacgcgc ttcatcgaca atttctgcca gtgctttttc agtcatcccc ggatagtgtt
     1141 cgagggttaa tgctttgacg ctgtcgccaa ggttatggtt gcgcacctta ccggtaaagg
     1201 tgactaccgc accgtcttca tcacgctccg ccagccacgg atactcttct cccacgctga
     1261 atggctgcgg gccgacaaca attttggttt ctgccatctt aacctccggt taccggcggg
     1321 aagaaagcca cttcgtcgcc gtcagtcagc ggatggtcaa gactcaccag cgtctggttg
     1381 acggcagcca gcaatttgcc atcttccagc gccagcgccc agcgatcgct ctgcgcagcc
     1441 agatgttggc gtaacgcttc aacggttggg aaatccgcag ccacttcggt tgcatctgtt
     1501 cccaccaact cgcgcacctg ggcgaaaaaa agaactttaa tcatccgctt ccaccttaaa
     1561 gtcacccgac ttgccgccgc tcttcgccag caaacgtacc ggaccaatta ccatatcttt
     1621 ttgcaccgct ttgcacatgt cataaatggt cagcgccgcc acggaggccg cggttaacgc
     1681 ttccatttcg acaccggttt tcccggtcag gcggcataag gtttctatac gtacccgatt
     1741 gtgctccggc tcggcctgta aattgacttc aactttgctg agcatcagcg ggtgacacag
     1801 cgggatcaga tcccaggtgc gttttgccgc ctgaataccg gcgatacgcg cagtggcaaa
     1861 tacgtcgcct ttgtggtggc gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt
     1921 gacaaaggct tccgcccgcg cttcacgcac ggtttccgct ttggcggaga catccaccat
     1981 gtgtgcttcg ccagcggcgt tgatatgggt cagttgcgac atacttattt cttcaaatgt
     2041 ggatggaaat tgcatggacg tgtacgggca tcgagctgcg gcgcgatgat attttcccac
     2101 gcggtgcggc acgctttggt cgaacccggc atggcgaaaa tcagcgtttt gttagcgacg
     2161 cccgctaccg cacgagattg caacgttgag gtgccaatct cttcaaacga caacatccgg
     2221 aacacttcac caaaaccttc aacttcacgg tcgaacaacg gcagcagcgc ttcgggagcc
     2281 tgatcacctt ccgtcaggcc agtaccaccc gtaatcaata ccacttgtac atcgtcgctg
     2341 gcgatccacg cagatacctg agcgcgaata gcgtagcggt tttctttcac aatggcttta
     2401 tcgacaacgt gatggcccgc ttcctgcgcc gaatcgcgca gatagtgacc ggaggtatcg
     2461 tcttcttcac cacgacgatt agaaaccgta agaatagcaa tacgggtcgg gataaattca
     2521 gtgcttacct gactcatctg atctctcctt ttgacgtttt agccgccaat gtacgataag
     2581 ttttgcgtaa taccggtgtt gttttgatgc aggaaatggg tctgtttctt ctcccgcagc
     2641 gccgctgaaa tacgcgcttc cagcgcctgt tgctgggtat cgtcttccag cagatcgcgc
     2701 aggttaacgc cgccttcacc aaacaggcag agatggagtt taccaatgga ggaaacgcgc
     2761 aggcggttgc aagtggcgca gaagtctttt tcatacggca tgataaggcc aatctctccg
     2821 gcgtaatccg gatgacaaaa gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg
     2881 atccagccgc gacgcagtag ctcgtcacgc agaacctgac cagagatgtg atgcttacgg
     2941 aagagctcgc tgccctcacc cgtttccatc aattcgatga aacgcagctg gatagggcga
     3001 tgctggatcc agttcagaaa ggtgtcgagc tgatgatgat taacatcacg catcagcacg
     3061 gtattgactt tgaccttttc aaaaccggcc tcaaatgcgg catcgatccc tgccatgacc
     3121 tggttgaatt tatcctgccc ggtaatggca tgaaactggt gagcgtcaag gctatcaacg
     3181 ctgacgttga tgccagtaag tccagcatcg cgccagttcg ccacatcgcg ttccagacgg
     3241 taaccattgg tggtgaccgc aatctggcgg atagcgtcgt tttcccgcac agcggcgatg
     3301 atatcggtaa agtcgcggcg taaagacggc tcaccacctg tcagacggac tttttcggtg
     3361 ccaagactgg cgaaggcgcg cgtaacccgg cgaatttcat cgacggtaag aaagccttta
     3421 ttggtgacgc cgctcggttt gtagccatcc ggcaggcagt aggtgcaacg aaagttacac
     3481 acatcggtaa tcgacaggcg caagtagtaa aacttacgcg caaatgcatc agtcagttgt
     3541 gaagccatgt acacctttcc agatacggga ggcgaagtca tttcttcctt cgccctggtg
     3601 gcaatcttta cgagcattgt cacggccaaa gcaccgtatc agttgaccca ggtgcagagg
     3661 ctagagtgtt tagtggttaa gccgatacta gcgtgtaaat gagattttta ccatccacac
     3721 tttcgctatg taatcatgta tatagcgtca tgatcgtgct atttcgctac agaataaggg
     3781 aaaatggcgg ttttactttg atatacatca tcaggaaggc atagcgtaca tcgtccttta
     3841 gaggtagaag aaaatagttt gtatcctcaa tattggcagg ttaattgctg tttccccgca
     3901 atttgcgcta ctgtagcgcg gataatctga ctccaggaaa ctatatgcgc aatcgtacgc
     3961 tggctgacct tgatcgtgtc gttgctctcg gcggagggca tggactggga cgcgttctct
     4021 catcactttc gtctttgggt tctcgtttaa cgggtatcgt caccaccacg gataatggtg
     4081 gctcgacggg gcgtattcgc cgctcagaag gcggtattgc ctggggcgat atgcgtaact
     4141 gcctcaacca gctgataact gaacccagcg tcgcttccgc aatgtttgaa tatcgtttcg
     4201 gcggtaatgg cgaactttcc ggacacaacc ttggaaacct gatgctgaag gcactggatc
     4261 accttagcgt gcgacctctt gaagcgatca atctgattcg caatctgctg aaagtggacg
     4321 cacatttgat tccgatgtcg gagcatcctg ttgatctgat ggcgattgac gatcaggggc
     4381 atgaagttta cggcgaggtc aatatcgacc agttaactgc gccgattcaa gagttattgt
     4441 taacgccgaa tgtacccgca acccgtgagg cggttcacgc tatcagtgaa gcggatctca
     4501 tcattattgg gcctggcagt ttttacacca gcctgatgcc aattctgctg ctgaatgaaa
     4561 tcgcacaagc attacgccgc acgccagcgc cgatggtcta tatcggcaat ctggggcgtg
     4621 agttaagttt acctgcggct aatctgaagc tggaaagcaa gctggcaatt atggagcagt
     4681 atgttggtaa aaaggtcatt gatgcggtca tcgtcgggcc caaagtggat gtctcggcgg
     4741 tgaaagagcg gattgtgatt caggaggtgc tggaggccag cgatattccg tatcgtcatg
     4801 accgccagtt gctgcatagc gcgctggaaa aggcattaca ggcactgggt taatgtggcc
     4861 gagattgctg accaggcttc tggtgcaatg cgacctgttc ccctcaccct aaccctctct
     4921 ccggaggggc gaggggaccg ttcgagcact tttttgactt gatcgttaat ctgagtcgga
     4981 agtacattcc ggctttttca tcttgtcacg acgcggcaat aaacagctca cgcagctgat
     5041 gcaactggtc acgaatttgc gccgcttctt cgaactccag attctgcgcg tgttgcatca
     5101 tcaacccttc cagttcatgg atcttctgct gcaacgcttt aggcgacata tccatcggca
     5161 cattatccgg ctcaacaatc gggcgcgatt ttcctctgcc cttcgctttg gttttggcaa
     5221 tgttctgccc cagcgccagg atatcgacca ctttcttgtt caagccttgc ggcgtaatgc
     5281 cgtgttcctc gttgtacttc tgctgtttct cgcggcgacg ttcggtttcg ccaatcgctt
     5341 tcgccattga tggggtgatc ttatcgccgt agagaatcgc tttaccgtta acgttacgtg
     5401 ccgcacgacc aatggtctgg atcaacgaac gttcggaacg caggaagcct tctttgtcag
     5461 cgtcgaggat cgccaccagc gacacttccg gcatatccag accttcgcgc agtaagttga
     5521 tccctaccaa tacgtcgaac tcacccagac gcaagtcgcg gataatctcc atacgttcga
     5581 cggtgtcgat atctgagtga agataacgca cgcgctcacc gtgttcttcg agatattcag
     5641 tgagatcttc cgccatccgc ttggtcagag ttgtaaccag tacgcgttcg ttaattgccg
     5701 ctcgctgacg aatctccgaa agaagatcat ccacctgtgt tgctaccggc cgcacttcga
     5761 taatcgggtc gagtaagcct gttggacgca ccacctgatc caccacatca ccaccggatt
     5821 tttccagctc gtaattaccc ggcgtcgccg aaacatagat ggtttgcggc gctaatgctt
     5881 cgaactcttc aaatttcagc ggacggttat ccagcgctga tggcaggcgg aagccgtact
     5941 ccaccagagt ctctttacgc gcccggtcac cgcgatacat gccaccaatt tgtggaatgg
     6001 tgacgtgaga ttcgtcgacg accagcagcc catcggcagg caggtaatca aacagcgtcg
     6061 gcggtggctc acccggtcca cgaccggaga ggaagcgcga gtagttttca atccccgaac
     6121 agtagcccag ctcgttcatc atctccagat caaactgggt acgctgggtc agccgctgct
     6181 cttccagcag tttgttgttt tccaacagca ctttgcgtct ggcggccagt tcttctttga
     6241 tctcctccat cgcctgtacg atgcgctcac gtggcgtgac gtagtgcgtt ttcgggtaga
     6301 tggtaaaacg tggaatagtg gaaacaatct gcccggttag cgggtcaaat aacgacaatc
     6361 gttccacttc ctcgtcaaac agttccacgc gaagtgcaat gtcatccgat tctgccggga
     6421 agatatctat cacctcacca cgaacgcgga aagtaccgcg ctggaatgcc tgatcattac
     6481 gagcgtattg cagctccgcc agtcggcgca ggatcgcgcg ctgatcgata atcatgccga
     6541 ccgtgagatg gagcatcatc ttgagatata aatcaggatc gcccagacca taaatcgcgg
     6601 aaacagacgc caccacaacc acatcacgcc gctccagcat cgctttggta gcggacaaac
     6661 gcatctgctc aatatgttcg tttaccgagg catctttctc aatgaaggtg tcggaactcg
     6721 gtacataggc ttccggctga tagtagtcgt agtaggagac gaaatattcc accgcgtttt
     6781 ccgggaagaa ctctttcatt tcgccgtaca gttgggccgc cagcgttttg ttgggcgcca
     6841 gtaccatggt tgggcgctga aggtcagcaa tgacattggc gatggtgaag gttttcccgg
     6901 agccagttac accgagtaac gtctggtgcg ccaggccatc ttccagcccc tcttcgagac
     6961 gtcgaatcgc ctctggctga tcgccagaag gtttaaaagc ggaattcagt ttgaacggtt
     7021 tactcatgag tcgctacctg aaggagttgg gcgggcaggt atgtaatttt actcgtcgta
     7081 cttaattatg ccaacaaatt atactggata aaaaaacagt ttctcaccat aatatttctg
     7141 ttacagcgta aactccgctg tcaatcatga gcaaaattta ctctgtggcg agataaaact
     7201 ccggccttac cgggttatcc ccaaagcaac ggctttttta acatttgtca agatgagtga
     7261 tgacagtttt atggcaagag atgcctcttc agtgactgcc attgcaatta tctaaccagt
     7321 taaaaaataa aagatatttc tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt
     7381 attctctcgc ttgcctcgtg ttttctaact ctaatacaca tggttatcca caggaatagt
     7441 ggataactgt ctgcagcccc tatcccccgc cgcctgggca attcccacat tcaccgcatg
     7501 atgcggaggt aaaatttttt tcgtgatttt tttagaattt aattggttaa attgcagtca
     7561 atcgaagacg cgatctcgct cgcaatttaa ccaagtacag gatgactaca ttaaggcaag
     7621 attgatgtac tttccggttg ccgcattttc tggattttct gcaagccagg gaatttcgcc
     7681 cagcagcggc gcgggaatca tgcgggtgag cgtggtcata tattcagcgt gacgttttcc
     7741 cggaggcgtg acatcgttcg ccacccaacc cgccagagtc agtccggcgt gttgtattgc
     7801 ctgtgccgtc aacatcgcgt gattaataca gccgagtttc acaccaacta ccagtatcac
     7861 cggcagttgt tcctgtgtta cccaatccgc aaaagtgaaa gtgtcagaaa gcggcgtaaa
     7921 ccagccgcca gcaccttcca ctaacaccca gtcagcctgt tgttcaagcg cgcgtaatcc
     7981 ggcgctcatt accaatgatt ctatcggcct gccctcttgt gcgctgatga tgtgcggcga
     8041 agtgggttct gcgaaggtgt aaggatttac cgttgcgtaa tccagctgca ggctgctgtt
     8101 gcgctgtaac gccagcgcgt cgctattacg taagccttcc ggggtctttt cgctgccaga
     8161 ggcgaccggt ttataacctg ccgtccggta gcctactgcc tttgcggctt gtaaaagtgc
     8221 acaactggcg acggttttcc ccacttcggt atccgttccg gtgacaaaat aacgtttact
     8281 cacgagcaat cactcccaaa aaaagatgat acgtcagagg atatcgcccc tgtttttgtg
     8341 gccaggccag ttgcaatcgc tgcaactgcg aacgcgttaa tatccgcggg tcgcgccctt
     8401 catgaagatg cgtggcaccg atgcctttca gcgaacgcat ggcactgagc gcatcatcaa
     8461 accacagcgt gatgggctga atatgatgtt gataatgcac gccgttcagt gactgttcga
     8521 tttcatctgg cggtaaaaag cgattagcat gcggacgctc gtccaccgcc tgccacgcct
     8581 gatgcagttc gggtaacgat ccctgcacca gcgtggtaaa cgcgaccacg cctccggggc
     8641 gcaccacccg atacagctcg cggagtgccg tggataaatt accgcaccac tgcaccgcga
     8701 gattgctcca tgcaagatcg aaggttgcag tcgctaacgg cagggattcg atatctcccg
     8761 ccagataatg gtctgcggca tccttctggc gtgcctgaac aagcattggc ggcgagagat
     8821 ctaacgccgt cacctgcgcg tgacgttccc gccaatgacg gctcatccag ccaggtccac
     8881 aacccgcatc cagtacgtgg gtgtatttac gctgtgggag cattgccagt aagacgtcag
     8941 cactctggcg ctgtagatcc gcatgttgct catagtgcgc ggctgcccga ccaaatgccg
     9001 ctgcaatggc ttgtttatta accgttgtca tgcagcacct ccagcagact atcgatatcc
     9061 tgcatttcat gcgctgcggt tagcgttaag cgcagtcgcg cagtaccagc gggtacggtt
     9121 ggcgggcgaa tcgccgtgac ccagcaacct tgctggcgca gtttttctgc cagttgtaac
     9181 gcacggctgt tatcaccgac gatcaatggc tggatggcgc tccatgaatc agcaagcgta
     9241 aacggcaaat cctgcactcc ggcacgaaaa cgcgtaatga gtgccgccag tttttcgcgc
     9301 cgtgcatcac cctcatcact gcgaatgacc gccagcgacg cacgtaatgc ctgcgcctga
     9361 gcgggcggca tactggtgct gtagataaga tggcgggcga attgcagcag ataatccgcc
     9421 accgtattgg agcaaagcac cgctgccccg ctgacgccaa atcctttgcc aaaagtcacc
     9481 accagcaatt ctggttttac cttttgcagc cagcagctgc cacgcccctg ctccccgata
     9541 acgcccgtgc cgtgggcgtc atcgaccatc aaccagccat cgtgctgttg cgttacctgc
     9601 tggatttcct ccagtggcgc actatcgccg tccatgctga acacgccttc tgtcactacc
     9661 agttgctgcc ccggacaggg ggaagcaagc agtcgcgcca gatgagtgac atcgttatga
     9721 gcaaaacggc gaagcggcga cgggcttaaa cttgcagcct ccagcaatga ggcatggcta
     9781 agccggtcgg caacaatacg gtcctctttc gccatcatcg cggtaataac tgcctggtta
     9841 gcggcaaaac cagagataaa cagcagtgcc cgcgaatagc ccagccactc ggccagttct
     9901 tcttccagcg cctgatgcgc cacgctataa ccgctgacgt gaccggagcc gccgctaccg
     9961 acgccaaatt gctccgcact cagcttccag gcacggataa tttgcggatg atggcttaaa
    10021 cctaaataat cgttactgga aaagttcaga tactggcgat cgtccgccac cagccagcgt
    10081 ccggctcctt gcgccactgg ataacggcga cgcagggcat cggcagcacg ccgcgcatcg
    10141 agcgccgcgt tgattttctc ctgccagctc atagtgctgc cgcgttgtaa tattcgtcag
    10201 tgtccggggt catcagcgcc tgctccagac gctgctgttg ttcgttatcg cccgccagca
    10261 cggcagtttg ctgcggattt agccccagtt tgcggaacag ttgcaggtct ttatcttctt
    10321 ccggattcgg cgtggtcagc agtttgcaac cgtagaaaat cgagtttgcg ccagccataa
    10381 agcacatcgc ctgagtctgt tcgttcatct gctcgcgtcc ggcagaaagg cgtacgtaag
    10441 aggtcggcat catgatccgc gcaactgcaa tggtgcgaat aaaatcaaag gcatcgacat
    10501 catcgttatc agcaagcggc gtgcctttca ccttcaccag catgttgatt ggtacgcttt
    10561 ccggcggcgt tggcaggttt gccagttgca gcaataatcc ggcgcgatct tttactgttt
    10621 cgcctaagcc cacaatgccg cccgagcaga ctttgatccc ggcgtcgcgc actttttcca
    10681 gcgtatcgag gcgttcctga taggtacgcg tggtgatgat attgccgtaa aactccggcg
    10741 aggtgtcgag gttgtggttg tagtaatcca gcccggcgtt cgcaaggcgc tgcgcctgag
    10801 attcactcaa cgtacccagc gtcatacacg cctccagccc catcgctttt actccctgca
    10861 ccatttgttc caggtagggc atatcgcgtt cgtggggatt cttccacgcc gcgcccatgc
    10921 agaagcgcgt cgatcctgcc gctttcgctt tgcgcgccga ctccagcacc tgttcaactt
    10981 ccatcaaccg ctcggcttcc agcccggttt tgtagcgcga gctttgcggg cagtatttgc
    11041 aatcttccgg acaagctccg gtcttaatcg acagcaaagt gctgacctgc acctgacgtg
    11101 gatcgaaatg ctgacgatgc acctgctgcg cttcaaacag cagatccagc aacggttttt
    11161 caaataattc tgtgacttgc gacaatgtcc agcgtgggcg gtgagccata gggcttctcc
    11221 aaaacgtgtt ttttgttgtt aattcggtgt agactcgtaa acctaaatct tttcaatttg
    11281 gtttacaagt cgattatgac aacggacgat cttgcctttg accaacgcca tatctggcac
    11341 ccatacacat ccatgacctc cccgctaccg gtttatccgg tggcgagcgc cgagggttgc
    11401 gagctaattt tgtctgacgg cagacgcctt gttgacggta tgtcgtcctg gtgggcggcg
    11461 atccacggtt acaatcaccc gcagcttaat gcggcgatga aatcgcaaat tgatgctatg
    11521 tcgcatgtga tgtttggcgg tatcacccat gcgcccgcca ttgagctgtg ccgcaaattg
    11581 gtggcaatga cgccgcaacc gctggagtgc gtttttctcg cggactccgg ctccgtagcg
    11641 gtagaagtgg cgatgaaaat ggcgttgcag tactggcagg ccaaaggcga agcgcgccag
    11701 cgttttctga ccttccgcaa tggttatcat ggcgatacct ttggcgcgat gtcggtgtgc
    11761 gatccggaca actcaatgca cagcctgtgg aaaggctatc tgccagaaaa cttgtttgct
    11821 cccgccccgc aaagccgcct ggatggtgaa tgggatgaac gcgatatggt gggctttgcc
    11881 cgcctgatgg cggcgcatcg tcatgaaatc gccgcagtga tcattgaacc gattgtccag
    11941 ggcgcaggcg ggatgcgcat ctaccatccg gaatggttaa aacgaatccg caaaatgtgc
    12001 gatcgcgaag gtatcttgct gattgccgac gagatcgcca ccggatttgg tcgtaccggc
    12061 aaactgtttg cctgtgaaca tgcagaaatc gcgccggata ttttgtgtct cggtaaagcc
    12121 ttaaccggcg gcacaatgac cctttccgcc acgctcacca cgcgcgaggt tgcggaaacc
    12181 atcagtaacg gcgaagccgg ctgctttatg catgggccaa cttttatggg caatccgctg
    12241 gcctgcgcag cagcaaacgc cagcctggcg attctcgaat ctggcgactg gcagcatcag
    12301 gtggcggcta ttgaagggca gctacgcgag caacttgccc ccgcctgtga cgccgaaatg
    12361 gttgccgatg tgcgcgtact gggggcaatc ggcgtggtcg aaaccactcg tccggtgaat
    12421 atggcagcgc tgcaaaaatt ctttgtcgaa cagggtgtct ggatccggcc ttttggcaaa
    12481 ctgatttacc tgatgccgcc ctatattatt ctcccgcaac agttgcagcg tctgaccgca
    12541 gcggttaatc gtgcggtgca ggatgaaaca tttttttgcc aataacggga agttagcgtg
    12601 agggtttctg gctacacttt ctgcaaacaa gaaaggaggg ctcatgaaac tcatcagtaa
    12661 tgatttacgc gatggcgata agctgccgca tcgtcatgtc tttaacggca tgggttacga
    12721 tggcgataat atttcaccgc atctggcgtg ggatgatgtt cctgtgggaa cgaaaagttt
    12781 tgttgtcacc tgctacgacc cggatgcgcc aactggctcc ggctggtggc actgggtagt
    12841 tgttaattta cccgcggata cccgcgtatt accgcaaggg tttggctctg gtctggtagc
    12901 aatgccagac ggcgttttgc agacgcgtac cgactttggt aaaaccggat acgatggcgc
    12961 agcgccgccg aaaggcgaaa cccatcgcta catttttacc gttcacgcgc tggatgtaga
    13021 acgtattgat gtcgatgaag gtgccagcgg cgcgatggtc gggtttaacg ttcattttca
    13081 ctctctggcg agagcctcga ttactgcgat gtttagttaa tcactctgcc agatggcgca
    13141 atgccatctg gtatcactta aaggtattaa aaacaacttt ttgtcttttt accttcccgt
    13201 ttcgctcaag ttagtataaa aaagctgaac gagaaacgta aaatgatata aatatcaata
    13261 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc
    13321 agtcactatg aatcaactac ttagatggta ttagtgacct gtaacagagc attagcgcaa
    13381 ggtgattttt gtcttcttgc gctaattttt tgtcatcaaa cctgtcgcac tccagagaag
    13441 cacaaagcct cgcaatccag tgcaaagctt tgtgtgccac ccactacggc ccgcataacc
    13501 agtaagaaga tagcagtgat gtcaaacgac gcagcagtct tcttttcttt cacactttcc
    13561 cccacccagc atacatacct ttctgccaca gctgaaatga atgtctatta tgagcaagga
    13621 gaggaaattc gtaattgtca tcatcaatgg cgatttgctg gtactatctg tgttaagtaa
    13681 taggaaaaca agtaactgga gaaagatttg atttacacac tattatagca gttaatagtt
    13741 taagctattg acactttaac tattttgcat aaagttaaac ataacaaccc cataaattga
    13801 taatgcaaga gagtactgaa ctaagtattt tagctctacc atattgccca ggcagtataa
    13861 tatatgatac attaaaaaat ta
//
LOCUS       DAOTRK010000122        13483 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.123, whole genome shotgun sequence.
ACCESSION   DAOTRK010000122 DAOTRK010000000
VERSION     DAOTRK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.123"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..507
                     /locus_tag="QIE41_003541"
     CDS             <1..507
                     /locus_tag="QIE41_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3532386.1"
                     /translation="IAPYVNLFRADLSFSKLDLINFEHADLSRVNLNKATLQNINLID
                     SKLFFTRLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKAWMFNTRLYRVNF
                     DEANVQGMGISILREEENIPINSDTLITLQKFFEEDCTSHTGMSQTEDNIHAVAMKIT
                     ADIMRDAD"
     gene            complement(661..2310)
                     /locus_tag="QIE41_003542"
     CDS             complement(661..2310)
                     /locus_tag="QIE41_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDS3532387.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2461..3810)
                     /gene="ghxP"
                     /locus_tag="QIE41_003543"
                     /gene_synonym="yjcD"
     CDS             complement(2461..3810)
                     /gene="ghxP"
                     /locus_tag="QIE41_003543"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDS3532388.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4356..4820)
                     /gene="soxR"
                     /locus_tag="QIE41_003544"
     CDS             complement(4356..4820)
                     /gene="soxR"
                     /locus_tag="QIE41_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDS3532389.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4906..5229
                     /gene="soxS"
                     /locus_tag="QIE41_003545"
     CDS             4906..5229
                     /gene="soxS"
                     /locus_tag="QIE41_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDS3532390.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5232..6818)
                     /gene="pdeC"
                     /locus_tag="QIE41_003546"
     CDS             complement(5232..6818)
                     /gene="pdeC"
                     /locus_tag="QIE41_003546"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDS3532391.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVVVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIGQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7248..7529
                     /locus_tag="QIE41_003547"
     CDS             7248..7529
                     /locus_tag="QIE41_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDS3532392.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7628..8164)
                     /gene="ssb1"
                     /locus_tag="QIE41_003548"
     CDS             complement(7628..8164)
                     /gene="ssb1"
                     /locus_tag="QIE41_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDS3532393.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8419..11241
                     /gene="uvrA"
                     /locus_tag="QIE41_003549"
     CDS             8419..11241
                     /gene="uvrA"
                     /locus_tag="QIE41_003549"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDS3532394.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11276..11632)
                     /locus_tag="QIE41_003550"
     CDS             complement(11276..11632)
                     /locus_tag="QIE41_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDS3532395.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11636..12052)
                     /locus_tag="QIE41_003551"
     CDS             complement(11636..12052)
                     /locus_tag="QIE41_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDS3532396.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12163..12876)
                     /gene="aphA"
                     /locus_tag="QIE41_003552"
     CDS             complement(12163..12876)
                     /gene="aphA"
                     /locus_tag="QIE41_003552"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDS3532397.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            13277..13483
                     /locus_tag="QIE41_003553"
                     /pseudo
     CDS             13277..13483
                     /locus_tag="QIE41_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3544 a         3481 c         3253 g         3205 t
ORIGIN      
        1 atagcgccgt atgttaattt atttagagcc gatcttagtt tctctaaact cgatttaatt
       61 aactttgaac atgctgatct gtctcgcgtc aatctgaaca aagcaactct ccagaatata
      121 aacttaattg atagcaaact cttttttacg cggctgacaa atacattcct tgaaatggtt
      181 atatgtaccg gctctaatat ggctaatgtt aattttaata atgccaattt aagcaattgc
      241 catttcaact gttctatttt aacaaaagcc tggatgttta atacccgtct ctatcgggtt
      301 aatttcgatg aggctaacgt gcagggaatg ggcatttcca ttctccgtga ggaggaaaat
      361 atccccatta atagtgatac cctgataaca ctacagaaat tctttgaaga agattgtacc
      421 tctcatactg gcatgtcaca aactgaggat aatattcatg cagtcgctat gaagattact
      481 gcagatatta tgcgagatgc agattgaggt taagccgaag aacagccaat gaattgactt
      541 taaaagagag ataacttatt tgttttagaa tcatgtatgt tagcccgagc taacctgtta
      601 aaggtgcaaa agattgcacc tttttcaaat tacctcgggc ctggttacgt cgctttcggc
      661 ttactgattt tcctcaatca acaacgcttc aagcaaatcg agatcgtgca gtaatttttg
      721 cagcgtttca ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga
      781 acgcaatgcc gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac
      841 gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg acgcgagaac tgacctccgt
      901 gagcagctgg ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg
      961 gatcgccaca atcgccactt ctgccgttgc cgctcgcgca atacgttcct ctttcaattg
     1021 ttgcgaatgg tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac
     1081 aaacagcgag aagagaatga caccagccgc caggaatacc agctcatagc gcgccgggaa
     1141 gacgttacca tccggcaaga gcagcgggat agagagcaca ccagccagag tgatcgcccc
     1201 acgcaccccg gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat
     1261 cggcttcttt ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg
     1321 gaccagcatc agcgccgcat aaatcagaat gatatcggta aacagcatcc agatttcgac
     1381 gttagggtcg atttctgccg ccatcagcga cgtctccaga atacccggca gctgcagacc
     1441 taacagcagg aataccatgc cgttaaagac aaattccagc atcgcccagg tgctgtttgc
     1501 gcgcaggcgc attgccagcg gcgcacggcg catcacaccg gagcgggtga tggtcatccc
     1561 ggcagcaacc gcagcgagga tgccggaaac gccaatatgt tcggcaatca gataagaagc
     1621 gaatggcagc aggaacagca ggacgatctg cgtcgcgggt tcatcaccgc cccagcggct
     1681 gaggaatcgc agcgagcgac cgtacagcca gctcaccaca aaaccggcga gaataccgcc
     1741 aatggctact ttcataaatt cgaccgtcgc accgccaacg gtgaagatca tcgtccccat
     1801 cgccactgct acggcaaact tcaacgacac caggccagag gcgtcattca tcaacgcttc
     1861 gccctgcaaa atgcccatga tttttttcgg gatgcgccct tcgccaacaa tcccggagag
     1921 cgccacagca tcggtcggag aaagcaccgc cgccagcgca aaggcgggga tcagcggaat
     1981 gcccggcacc acccagtaaa taaggaagcc aatgccgacc acggtgacca ccaccagcgc
     2041 cagcgcgagg ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca
     2101 gccatcagcg aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc
     2161 cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg
     2221 aagcgggatc tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt
     2281 cattatcagt atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat
     2341 ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
     2401 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc atatgaaaga
     2461 ttagatagcc cagccacccg catagaaggt caccagcgcc acagcgataa cgaccgtacc
     2521 gatgttcaac ttgcgccatt cgccggaaac cagacgacca atcaccagag tcgcgaagcc
     2581 gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
     2641 cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttactca gcatcagcag
     2701 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag agagcggaga
     2761 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaacc cggttttacc
     2821 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag
     2881 gccagagaaa acgctgctca tggagtcggt ggtcagtgct ttcccaccgt caatgatctg
     2941 cccatcttta tccagcaggt tcgcctgacc ggcgacggcg cggatagttc cggtggcatc
     3001 aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
     3061 cataatgtcc aggctaccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc
     3121 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
     3181 aatggtcagc agaatgccac cagggacttt cagtttttcc aggccgatga tcaccgccag
     3241 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
     3301 atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca gcaggaacag
     3361 accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
     3421 acggataccc gtggcagaaa ttaccgtaaa cagtacaccc atcaggaaca cagcacccag
     3481 cgcgacaggt acgctaatat gttgccccag caccagactg aatgcggtaa acgccgtcag
     3541 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
     3601 gagtccggca accagacagg tcgcaacgaa aactgccgca ggcgggaagc ctgctttacc
     3661 caacatgcct ggaacgacga taaccgagta gaccatcgcc agaaatgttg ttaacccggc
     3721 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
     3781 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
     3841 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
     3901 gcgacaaatt ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
     3961 gtaaaaaaag caaacgatta tccggcgatc atagcggctt tttcaaccga agtttgctgc
     4021 ttttttctct tttattgtct tgacgatgaa aaaccaggca attgatgcgc aaacgttatc
     4081 gtcgatgcgc aagattgcag cgttatcgag ccatataccg ttcaggattg cgataaaaaa
     4141 tcgtcttaga tgacaaaaat attgcggcta aaaagtgtac ttttactctg gcgaagaaac
     4201 gccggtgaca tacaattaaa gcatcaacac caaccggaac ctccaccacg tgctcgaatg
     4261 aggtgtgttg acgtcggggg aaaccctcct gtgtaccagc gggatagaga gaaagacaaa
     4321 gaccggaaaa caaactaaag cgcccttgtg gcgctttagt tttgttcatc ttccagcaag
     4381 cgtgcgcctg tgccttcttc tcctaagcgg tcgcccgggt tacgcaatgg gcaatcactg
     4441 cgcgaaaggc agccacaacc aatacatccg tccagctcgt cacgcagcgc cactaaggta
     4501 tgaatgcgcc gatccaactc ttctcgccat tgggacgaaa gctgtttcca ctctttcgca
     4561 cttaatgtat gcccttcggg caacacgccg aatgcttcac gaatggtcgc cagcggaatg
     4621 ccaatacgct gagcaatttt gatgattgcg acatatcgca acacatcacg tttatatcgc
     4681 cgctgattgc cactattacg gatactgcta atcaaccctt tgctttcata gaaatgcagc
     4741 gccgataccg ccacaccgct gcgttttgcc acttcgccgg gggttagcag cgctttaatg
     4801 cggggtaatt tcttttccat aaatcgcttt acctcaagtt aacttgagga attatactcc
     4861 ccaacagatg aattaacgaa ctgaacactg aaaagaggca gatttatgtc ccatcagaaa
     4921 attattcagg atcttatcgc atggattgac gagcatattg accagccgct taacattgac
     4981 gtcgtcgcaa aaaaatcagg ctattcaaag tggtacttac agcggatgtt ccgcacggtg
     5041 acgcatcaga cacttggtga ttacattcgc caacgccgcc tgttactggc cgccgttgag
     5101 ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg acctgggtta tgtctctcag
     5161 cagaccttct cccgcgtttt ccgtcggcag tttgatcgca ctcccagcga ttatcgccac
     5221 cgcctgtagt tttattgccc gcgcgttaac tcccgcgcgg gtaattgctc catccattgc
     5281 ataaacacct gcggcggcat cgccttcgca aagaaccatc cctgacaata gcgcacgccg
     5341 cgtttgcgca gccagttaac ctgctcctca gtttcgacgc cttcagcgat cgtttttaac
     5401 cccagactgt gcgccagctc gatgatgtgt tccgcaatca aatgactggt tttgtgggtg
     5461 gtcagcgtct caacaaacga tttatcgatt ttcaaaatat cgacattcaa tgatttaagg
     5521 ttatgcaagt tagagtagcc aataccaaaa tcatcaattg ccacttcgta accagcctgg
     5581 cggaacgcca gaataattgg tgtcattttg tcgacatcaa gaaatgcatg ttcagtcact
     5641 tcaaatttaa tttgctgtgg acgcaccgcg tattgctctg ttttctgatt ggttcgcgct
     5701 atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa tagaaacata gcgatcggca
     5761 tgtgttgcca ggtacgcgcc cagatcgcgg aagacattat caataacata atcagttacc
     5821 tgtccgatca tcccctcctt ttctgccagc ggaataaact ctgccggatt cattacttgc
     5881 ccctgctcac caggccaacg taacaatgct tcagcgccga tacatttttc tgttttgata
     5941 tcgattattg gctggtaata aaggcaaagt tgatgttttt cgagggcgcg ttgcaatttg
     6001 cgtttgggag acaaatagtt ttgccgaata cgtagccaga gtaataacag aacaagactc
     6061 cccaaaatac cggcgggtaa cgcaaaaata agatgattat aaaaatgcgc tataagacgt
     6121 tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg tcgaataaac tgtcgcatat
     6181 aaatagccat ttctttgtac ggttaaatca ttcaaatgaa tcagtggaga aaacgttgct
     6241 gccgaggcct cgttgcttaa cgagaaaaag gttttcgtta ccgtatcata cacaccccat
     6301 tgcaatgtcg gatcatcaga catcacttca ctccagaaga gagggttgat gaccaccaca
     6361 taatttcccc gctgcatata ggtcatttta tagccagaga aaaaaggcgt atcgcggtaa
     6421 taatagatag aaacgttagg ttcacgctta taatcggccg gtgcaatcgt atagccgttt
     6481 acaggcgcta tcagcgatga gcataaaaaa tggttatcac gggcatagat caattcatta
     6541 atataaagat agccacgaat aatattcaac attcgctttt gatgggctgg agtgcaaact
     6601 tgcccctgat agcgttctgc tgcatcgctc actaaatctg cctggcgaat gaccagctcg
     6661 gatttatcca gcgccagctg agcaaaagtt tggagctgac tattcacttc tgattttgcc
     6721 caaaggaagg caatccatag cgacagtatg atgggaaaga gaactaaaaa gataatgccc
     6781 ggcaacgcca gtaattggtg tcgtgcacga tgactcattg ctatcaattt cctgcctgtt
     6841 tatacccggt cattgcaagg cgagaaccct ggttatcgac gtctttagat aaatcttacg
     6901 gtataaatag cacttttgcc tgatttgaaa ttgatacctt tttaatcgtc acctaaataa
     6961 aaataattca ttgatattca aaactaattt cctacaggaa tgcagaggcg gtgggaagat
     7021 atccccttaa tgccgctgaa aaaacacata atctgtacgg caataaaagt cgcggcctaa
     7081 tctggctaac aaacaatcaa ttgtgatata gttcacaaaa ttaatgaaac aaacagagtg
     7141 tttctttttt gtgttctgca agccccgcga tagccagatt gtatcacgcc tcccgtgaac
     7201 aacgccgcgc tgtccgaggc atcggctctt ttcagaggaa ttgtttgatg gctaccctca
     7261 ccactggcgt ggttcttctt cgctggcaac ttcttagtgc cgtaatgatg tttctggcca
     7321 gcacactcaa catccgtttt cgtcggtctg attatgtcgg gcttgcagtg atcagcagcg
     7381 gtctgggcgt agtttccgcc tgctggttcg caatggggtt gcttggcatc acaatggcgg
     7441 atatcaccgc catctggcac aacatcgagt cggtgatgat agaagagatg aatcagacac
     7501 cgccacaatg gccaatgatt ttgacttgat atgtagaagc ctccaaaacg gaggcttctt
     7561 ttttacggct ggcagatgtt ttaatcatcc accttaaaac aatataacct attgttttaa
     7621 tgaaaaatca gaacggaata tcgtcatcaa agtccatcgg cggctcatta gacggtgctg
     7681 ccggagcaga ctgctgcggg cgagactgcg cgccgccgct gaactgattg ccgccctgcg
     7741 gctgctgagg ctgaccccaa ccgccctgcg gctggccacc accgatattg ccacctgcag
     7801 gagcgccacc accctgacga ccacccagca tctgcatggt gccgccaacg ttcaccacca
     7861 cttcagtggt gtagcgatcc tgaccggatt gatcggtcca tttacgggta cgcagctgac
     7921 cttcgatata aacctgagaa cctttacgca gatattcgct ggccacttct gccagtttgc
     7981 cgaacagcac aacgcggtgc cattcagtct gctctttcat ctcgccggtc gctttatcac
     8041 gccaggattc ggaagtagcc agcgtaatgt tggcaacagc gccaccattt ggcatgtagc
     8101 gtacttccgg gtcctgaccc agattaccaa cgagaataac cttgtttacg cctctgctgg
     8161 ccatgttcgt gtctcctgaa aaaaaatcgt tctgaataag tgtaaacgcg cgattgtacc
     8221 attaccaata gcgcttttac tatgttgtga cctcggttcc gggaaacaaa cctggccaga
     8281 cattgttaca caacactccg ggtaatgcat tccaatactg tatattcatt caggtcaatt
     8341 tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat gccaccgggc aaaaaagcgt
     8401 ttaatccggg aaaggtgaat ggataagatc gaagttcggg gcgcccgcac ccataatctc
     8461 aaaaacatca acctcgttat cccccgcgac aaacttattg tcgtgaccgg gctttcgggt
     8521 tctggcaaat cctcgctcgc tttcgacacc ttatatgccg aagggcagcg ccgttacgtt
     8581 gaatcccttt ccgcctacgc gcggcagttt ctgtcactga tggaaaagcc ggacgtcgat
     8641 catattgagg ggctttctcc tgccatctca attgagcaga aatcgacgtc tcataacccg
     8701 cgttctacgg tggggacaat caccgaaatc cacgactatt tgcgtttgtt gttcgcccgc
     8761 gtcggtgagc cgcgctgccc ggaccacgat gtaccgctgg cggcgcaaac cgtcagtcag
     8821 atggtggata acgtgttgtc ccagccggaa ggtaaacgcc tgatgctgct cgcaccaatc
     8881 attaaagagc gcaaaggcga acacaccaaa acgctggaaa atctggcaag ccagggttac
     8941 atccgtgctc gtattgatgg cgaagtctgc gatctttccg atccgccgaa actggaactg
     9001 caaaagaaac ataccattga agtggtggtt gatcgcttca aggtgcgtga cgatctcacc
     9061 caacgtctgg cagagtcgtt tgaaaccgcg ctggagcttt ccggtggtac cgcggtagtt
     9121 gccgatatgg acgatccaaa agcggaagag ctgctatttt ccgccaactt cgcctgccca
     9181 atttgcggct atagtatgcg tgaactggaa ccacgcctgt tttcgtttaa caacccggcg
     9241 ggggcctgcc caacctgtga cggccttggc gtgcagcaat atttcgatcc tgaccgcgtg
     9301 atccagaatc cggaactgtc gctggctggc ggtgcgatcc gaggctggga tcgccgcaac
     9361 ttctattact tccagatgct gaaatcgctg gcagatcact ataagttcga cgtcgaagcg
     9421 ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt tgtacggttc tggcaaagaa
     9481 aacattgaat tcaaatacat gaacgatcgt ggcgatacct ccattcgtcg tcatccgttc
     9541 gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga cggaatccag tgcggtacgt
     9601 gaagaattag ccaagtttat cagcaatcgc ccgtgcgcca gctgcgaagg aacccgtctg
     9661 cgtcgggaag cgcgccatgt gtatgtcgag aatacgccgc tgcctgctat ctccgacatg
     9721 agcatcggtc atgcgatgga attcttcaac aatctcaaac tcgcaggtca gcgagcgaag
     9781 attgcggaaa aaattcttaa agagattggc gatcgcctga aattcctcgt taacgtcggc
     9841 ctgaattacc tgacactttc ccgctcggca gagacacttt ctggcggtga agcccagcgt
     9901 atccgtctgg cgagccagat tggtgcgggc ctggttggcg ttatgtacgt gctcgatgag
     9961 ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt tgggtacgct tatccatctg
    10021 cgcgatctcg gtaataccgt gattgtggtg gagcacgacg aagacgcgat tcgcgccgct
    10081 gaccatgtga tcgacattgg tcctggtgcg ggtgttcacg gcggtgaagt ggtcgcggaa
    10141 ggtccactgg aagcgattat ggcggtgccg gagtcgttga ccgggcagta catgagcggt
    10201 aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga atccggaaaa agtgctgaag
    10261 ctgacaggcg cacgcggtaa taacctgaaa gacgtgacgc tgacgctgcc agtcggtctg
    10321 tttacctgca tcacaggggt ttcaggttcc ggtaaatcga cactgattaa cgacacactg
    10381 ttcccgattg cccaacgcca gttgaatggg gcgaccatcg ccgaaccggc accgtatcgc
    10441 gatattcagg gactggagca tttcgataaa gtgatcgata tcgaccaaag cccaattggt
    10501 cgtactccgc gttctaaccc ggcgacctat accggcgtgt ttacacctgt gcgcgaactg
    10561 tttgcgggcg taccggaatc ccgtgcgcgc ggctatacgc cgggacgttt cagctttaac
    10621 gtccgtggcg ggcgctgcga agcctgtcag ggcgatggcg tgatcaaagt ggagatgcac
    10681 ttcctgccgg atatctacgt gccgtgcgac cagtgcaaag gtaaacgcta taaccgcgaa
    10741 acgctggaaa ttaagtacaa aggcaaaacc atccacgaag tgctggatat gaccatcgaa
    10801 gaggcgcgtg agttctttga tgcggtgcca gctctggcgc gtaagctgca aacgttgatg
    10861 gacgttggcc tgacgtacat tcgcctcggg cagtccgcaa ccacgctttc tggtggtgaa
    10921 gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg gcaccgggca gacgctgtat
    10981 attcttgatg agccgaccac cggtctgcac ttcgccgata ttcagcaact gctcgacgta
    11041 ctgcataaac tgcgcgatca gggcaacacc attgtggtga ttgagcacaa tctcgacgtg
    11101 atcaaaaccg ctgactggat tgtcgacctg ggaccagaag gcggcagtgg cggcggcgaa
    11161 atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg aagcttcgca tacggcacgc
    11221 ttcctcaagc cgatgctgta atcgttaagg ccgctttccg agcggccttt tcctttcaga
    11281 gttgcaccag caatttacgt ttttcttccg gcagcaaatt caccgcctgc tgataagacg
    11341 catccaccag ataatagatt tgcgaatctg gcagcgaacc gtcgagatac acggtgctcc
    11401 agtgcgcttt attcagatgg cggcttggac gcacatcgct gtgctgctga cgtagcagct
    11461 ccgccagctc cgggctggtt ttcagcgaaa ccgccgggcg attttctact tctttcacca
    11521 tcgcaaacag tacatcttcc actttgatct gcgtcgcttt ccagtcgtta tgcacgctct
    11581 gttctgcgcc tggttttgcc atgcaatatt gtagcaactc cgaaatggtc attttttact
    11641 ccccttgtag tgtcgcgata atgcgacgcg atccgccgtg gatgcgatgt tcccccagcc
    11701 agatgccttg ccaggtgccg gtctgaatac gccctttatg caccggcaat acaagcgatg
    11761 ttcccagcat tgaggatttg atatgagaag gcatatcgtc tgctccctca tagtcatgct
    11821 cataatttcc gttgtcggga acggtgcgga ggaaaaaacg ctccatgtcg tggcgtacgg
    11881 tgggatcgca gttctcatta agtgtcagag aggcggaggt atgttgcagc aacagatgca
    11941 gtaaaccgat gttaacgcgc ggcatatcag ccagctgatt cagaatttca tccgttacca
    12001 gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt ttgataccac atgtgctgct
    12061 ccttgataaa actctcttaa tcagtttgca gcaagacaga gaaaggataa aggtgtgatt
    12121 aaaaaaacag cattcgggag agtgtcacgc tctcccgctc tgtcagtatt ctgaattgac
    12181 gatcacctct tcaccaaacg cacccgcttg tggcaagggt ttgtaggtag agttggaggc
    12241 gcgcagaatg cggataccac gagcgccgac atcgcgtgcg gcggtaatat cgttatcgga
    12301 atcgccataa aaaattcgga tatttttatc ctgcagccat tgcgattttg tattttgccc
    12361 tggtttatca cccgcaaaga tcaccggatt catattggtg gcaggaatat gaaaattatc
    12421 cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta cgaccagtca caaagaagat
    12481 cgcgtcaccg cggcgtacat gcatatcaat cagctggcga gcgacctctt ttggaatgct
    12541 gaattcatcc cagccattgt tcattttttc ccagaacaca ggatttttca gataatcttc
    12601 gctttctggc gagaaggttt ttttgccgcg ccagaagccc ggactggaaa aaagtaccgt
    12661 gtcatcgata tcaaacccca ccgccattgg cggacgccct gcgaggctat tttcaatttg
    12721 tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc ctggcaacgt tagtaccagg
    12781 gttaagcggc gaaggagatg aggccagggc aacagcggaa ctgtttagag cgaacaataa
    12841 gcaaacggca ctgattgcct gtgtgatctt gcgcatattt ttccctaaat agtcagtatg
    12901 ttgaaacttt ttattgtgag atttgttgca aaaaccatct gaccataacg ccagcagcag
    12961 gggaaaggaa ggattatctg cggtttttgt gatgaaaagc aggatgatta tatttaaccc
    13021 agtaaatacg ttcctgtcgt ttttttgtaa aaaaatgttt attcctcaat gaaggataca
    13081 ttaattttac gtttattatg atgaatcctc tgcaaatgag cgaaatttga tagaacattt
    13141 atctgctgta cgatttaatg aacttaatga tacagcatta caaaaacaat cgaagtttat
    13201 aaagatgatt tctgattgac cagaacccta ccatacaatg gtgactaatt ttattactct
    13261 cgaaggagat gatgatatga atatatcata tgtgaatagc aacaaaatga catctttacc
    13321 tgttgagcta gatgcactaa ataataaaga tatctcttat tccaaagatt tttcttatgc
    13381 caaagatctc tttttatata tagaaacaca gttaaaaatt gcaaaagatt tttgtagacc
    13441 aggagaggaa gtatcaagat ctattgcaaa taaagttttt cat
//
LOCUS       DAOTRK010000123        13463 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.124, whole genome shotgun sequence.
ACCESSION   DAOTRK010000123 DAOTRK010000000
VERSION     DAOTRK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.124"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1089
                     /gene="cpsG"
                     /locus_tag="QIE41_003554"
                     /gene_synonym="manB"
     CDS             <1..1089
                     /gene="cpsG"
                     /locus_tag="QIE41_003554"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDS3532398.1"
                     /translation="VTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPV
                     DETKRGRYQQINLRDAYVDHLFGYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKAL
                     GAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLF
                     DEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHA
                     FIKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRM
                     AAFPASGEINSKLAQPVEAINRVEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEP
                     VVRLNVESRGDVPLMEARTRTLLTLLNE"
     gene            1144..2538
                     /gene="wcaJ"
                     /locus_tag="QIE41_003555"
     CDS             1144..2538
                     /gene="wcaJ"
                     /locus_tag="QIE41_003555"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDS3532399.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            2540..4018
                     /gene="wzxC"
                     /locus_tag="QIE41_003556"
     CDS             2540..4018
                     /gene="wzxC"
                     /locus_tag="QIE41_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDS3532400.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVISYALGIVLKGQMALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            4090..5370
                     /gene="wcaK"
                     /locus_tag="QIE41_003557"
     CDS             4090..5370
                     /gene="wcaK"
                     /locus_tag="QIE41_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDS3532401.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQQNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRTLDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACQLTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGH
                     LPALNARLSEAVSRERQMGMRMVQSVLERIGEAK"
     gene            5367..6587
                     /gene="wcaL"
                     /locus_tag="QIE41_003558"
     CDS             5367..6587
                     /gene="wcaL"
                     /locus_tag="QIE41_003558"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDS3532402.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            6598..7992
                     /gene="wcaM"
                     /locus_tag="QIE41_003559"
     CDS             6598..7992
                     /gene="wcaM"
                     /locus_tag="QIE41_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDS3532403.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALSARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPGGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLHGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVAKINFRLPERRE"
     gene            8167..9060
                     /gene="galF"
                     /locus_tag="QIE41_003560"
     CDS             8167..9060
                     /gene="galF"
                     /locus_tag="QIE41_003560"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDS3532404.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            9433..10518
                     /gene="rfbB"
                     /locus_tag="QIE41_003561"
     CDS             9433..10518
                     /gene="rfbB"
                     /locus_tag="QIE41_003561"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDS3532405.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGQAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LANAKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            10518..11417
                     /gene="rfbD"
                     /locus_tag="QIE41_003562"
     CDS             10518..11417
                     /gene="rfbD"
                     /locus_tag="QIE41_003562"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDS3532406.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLEMDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARNAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            11475..12353
                     /gene="rfbA"
                     /locus_tag="QIE41_003563"
     CDS             11475..12353
                     /gene="rfbA"
                     /locus_tag="QIE41_003563"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDS3532407.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMVVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFVDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            12358..12906
                     /gene="rfbC"
                     /locus_tag="QIE41_003564"
     CDS             12358..12906
                     /gene="rfbC"
                     /locus_tag="QIE41_003564"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDS3532408.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSSTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPQSDRGIKWDDPSINISWPVD
                     SQVLLSAKDNKHPPLTKIEMYS"
     gene            12950..>13463
                     /gene="wzx"
                     /locus_tag="QIE41_003565"
     CDS             12950..>13463
                     /gene="wzx"
                     /locus_tag="QIE41_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157658977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O1 family O-antigen flippase"
                     /protein_id="HDS3532409.1"
                     /translation="MSKSEIRNVIHNAGYMMITQIALYVAPLFILSYLLKTLGVAQFG
                     NYALILSIVAYLQIITDYGFSFSASRAISQNREDKEYISKIYLSTMTIKLAICAFLFL
                     LLMLFLNLLPVQAELKQGILYGYLLVIGNTFQPQWFFQGIEKLKIIALSNVISRCAAC
                     LLVFIYVRNSE"
BASE COUNT         3438 a         3089 c         3530 g         3406 t
ORIGIN      
        1 gttactgcca gccataatcc gatggattac aacggcatga aactggtgcg cgaaggggct
       61 cgcccgatca gcggtgatac cggactgcgc gatgtccagc gtctggcaga agccaatgac
      121 ttccctcctg tcgatgaaac caaacgcggt cgctatcagc aaatcaatct gcgtgacgct
      181 tacgttgatc acctgttcgg ttatatcaac gtcaaaaacc ttactccgct gaaacttgtg
      241 atcaactccg ggaacggcgc ggcgggtccg gtagttgacg ccatcgaagc ccgctttaaa
      301 gccctcggcg caccggtgga gttaatcaaa gtgcataaca cgccggacgg caatttcccc
      361 aacggtattc ctaacccgct gctgccggaa tgccgcgacg acacccgcaa tgcggtcatc
      421 aaacacggcg cggatatggg cattgctttt gatggcgatt ttgaccgctg tttcctgttt
      481 gacgaaaaag ggcagtttat tgagggctac tacattgtcg gcctgctggc agaagcattc
      541 ctcgaaaaaa atcccggcgc gaagatcatc cacgatccac gtctctcctg gaacaccgtt
      601 gatgtggtga ctgccgcagg cggcactccg gtgatgtcga aaaccggaca cgcctttatt
      661 aaagaacgta tgcgcaagga agacgccatc tacggtggcg aaatgagcgc ccaccattac
      721 ttccgtgatt tcgcttactg cgacagcggc atgatcccgt ggctgctggt cgccgaactg
      781 gtatgcctga aaggaaaaac gctgggcgaa ctggtacgcg accggatggc ggcgtttccg
      841 gcaagcggtg agatcaacag caaactggcg caacccgttg aggcgattaa ccgcgtggaa
      901 cagcatttta gccgtgaggc gctggcggtg gatcgcaccg atggcatcag catgaccttt
      961 gccgactggc gctttaacct gcgttcctcc aacaccgaac cggtggtgcg gttgaatgtg
     1021 gaatcgcgcg gtgatgtgcc gctaatggaa gcgcgaacgc gaactctgct gacgttgctg
     1081 aacgagtaat gtcggatctt cccttacccc actgcgggta aggggctaat aacaggaaca
     1141 acgatgacaa atctaaaaaa gcgcgagcga gcgaaaacca atgcatcgtt aatctctatg
     1201 gtgcaacgct tttcagatat caccatcatg tttgccggac tatggctggt ttgcgaagtg
     1261 agcggcctgt cattcctcta catgcacctg ttggtggcgc tgattacgct ggtggtgttc
     1321 cagatgctgg gtggcatcac cgatttttat cgctcatggc gcggtgttcg ggcagcgaca
     1381 gaatttgccc tgctgctaca aaactggacc ttaagcgtga ttttcagcgc cggactggtg
     1441 gcgttcaaca atgatttcga tacgcaactg aaaatctggc tggcgtggta tgggctgacc
     1501 agcatcggac tggtggtttg ccgttcatgt attcgcattg gggcgggctg gctgcgtaat
     1561 catggctata acaagcggat ggtcgcggtg gcgggggatt tagccgccgg acaaatgctg
     1621 atggagagct tccgtaatca gccgtggtta gggtttgaag tggtgggcgt ataccacgac
     1681 ccaaaaccgg gcggcgtttc taacgactgg gcgggcaatc tgcaacagct ggtcgaggat
     1741 gcgaaagcgg gcaagattca taacgtctat atcgcgatgc aaatgtgcga cggcgcacga
     1801 gtgaaaaaac tggtccatca actggcggat accacctgtt cggtgctgct gatccccgat
     1861 gtctttacct tcaacattct ccattcccgt atcgaagaga tgaacggcgt accggtggtg
     1921 ccgctgtatg acacgccgct ttccggggtt aaccgtctga taaaacgtgc agaagacatt
     1981 gtgctggcga cgcttatcct gctgctgatc tccccggtgc tgtgctgcat tgcgctggcg
     2041 gtgaaactga gctcaccggg gccggttatt ttccgccaga ctcgctacgg catggatggc
     2101 aagccgatca aagtgtggaa gttccgttct atgaaagtga tggagaacga caaagtggtg
     2161 actcaggcga cgcagaacga tccgcgcgtc accaaagtgg ggaactttct gcgccgcacc
     2221 tcgctggatg aattgccgca gtttatcaat gtgctgaccg gggggatgtc gattgtcggt
     2281 ccacgtccac acgcggtggc gcataacgaa cagtatcgcc agctcattga aggctacatg
     2341 ctgcgccata aggtgaaacc gggcattacc ggctgggcgc agattaatgg ctggcgtggc
     2401 gaaaccgaca cgctggagaa aatggaaaaa cgcgtcgagt tcgaccttga gtacatccgc
     2461 gaatggagcg tctggttcga tatcaaaatc gttttcctga cggtattcaa gggtttcgtt
     2521 aacaaagcgg catattgata tgagcttacg tgaaaaaacc atcagcggcg cgaagtggtc
     2581 ggcgattgcc acggtgatca tcatcggcct cgggctggtg cagatgaccg tgctggcgcg
     2641 gattatcgac aaccaccagt tcggtttgct taccgtgtcg ctggtgatca tcgcgctggc
     2701 agatacgctt tctgacttcg gcatcgctaa ctcgattatt cagcgaaaag aaatcagcca
     2761 ccttgaactc accacgctgt actggctgaa cgtcgggctg gggatcgtgg tgtgcgtggc
     2821 ggtgtttttg ttgagtgatc tcatcggcga cgtgctgaat aacccggacc tggcaccgtt
     2881 gattaaaaca ttgtcgctgg cgtttgtggt gatcccccac gggcagcagt tccgcgcgtt
     2941 gatgcagaag gagctggagt tcaacaaaat cggcatgatc gaaaccagcg cggtgctggc
     3001 gggcttcact tttacggtgg ttagcgccca tttctggccg ctggcgatga ccgcgatcct
     3061 cggttatctg gtcaatagtg cggtaagaac gctgctgttt ggctactttg gtcgaaaaat
     3121 ttattgcccc ggtctgcatt tctcgctggc gtcggtggca ccaaacttac gttttggtgc
     3181 ctggctgacg gcggacagca tcatcaacta tctcaatacc aacctttcaa cgctggtgct
     3241 ggcgcgtatt ctcggcgcgg gcgtagcagg gggatacaac ctggcgtaca acgtggccgt
     3301 tgtgccgccg atgaagctga atccgatcat cacccgcgtg ttgtttccgg catttgccaa
     3361 aattcaggac gacaccgaaa agctgcgcgt taacttctac aagctgctgt cggtggtggg
     3421 gattatcaac tttccggcgc tgctggggct gatggtggtg tcgaataact ttgtaccgct
     3481 ggtctttggt gagaagtgga acagcattat tccggtgctg caattgctgt gtgtggtggg
     3541 gctgctgcgc tcagtgggga acccgattgg ttcgctgctg atggcgaaag cgcgggtcga
     3601 tatcagcttt aaattcaacg tattcaaaac ctttctgttt attccggcga ttgttattgg
     3661 tgggcagatg gcgggcgcaa tcggcgtcac gcttggcttc ctgctggtgc agattatcaa
     3721 caccattctg agttacttcg tgatgattaa accggtgctc ggttccagtt atcgtcagta
     3781 catcctgagt ttgtggctgc cgttttatct ctcgctgccg acactggtga tcagttatgc
     3841 gctgggcatt gtactgaaag ggcaaatggc tctgggcatg ctgctggcgg tgcaaatagc
     3901 cgcgggcgtg ctggcgtttg tggtgatgat tgtgctgtcg cgccatccgc tggtggtgga
     3961 agtgaagcgt cagttttgtc gcagcgaaaa aatgaaaatg cttttacggg cggggtgaat
     4021 ggttgtttta ggctacggca ataaaaacaa caacataacc tcacctggca tcaaatttat
     4081 gaggaaaaaa tgaaattact gattctgggc aaccacactt gcggcaaccg cggcgacagc
     4141 gccatcctgc gcggcttact tgatgccatc aacatcctca atccacatgc cgaagtggac
     4201 gtgatgagcc gctatccggt cagttcttcc tggctgctca accgcccggt aatgggcgat
     4261 ccgctgttcc tgcaaatgaa acaacaaaac agcgcggcgg gcgttgtcgg gcgcgttaaa
     4321 aaagtcctcc gtcgccgcta ccagcatcag gtattgctct cacgcgtcac cgacactggc
     4381 aagctgcgta atatcgccat cgcccaggga ttcaccgact tcgtgcgctt gctgtcaggt
     4441 tacgacgcca ttattcaggt cggcggatcg ttttttgtcg atctctatgg tgtgccgcag
     4501 tttgaacacg cactttgcac gtttatggcg aaaaagccgc tgtttatgat tggtcacagc
     4561 gtcggtccct tccaggatga gcaatttaac caactggcaa actacgtttt tggtcactgc
     4621 gacgcgctga tcctgcgcga atcggtcagc cttgaactga tgaaacgcag caatatcacc
     4681 actgcaaaag ttgaacatgg cgtcgatacc gcgtggctgg tcgatcacca cacagaagat
     4741 ttcaccgcca gctatgccgt tcaacactgg ctggacgttg ccgcacaaca gaaaacggtg
     4801 gccattaccc tgcgcgaact ggcaccgttc gacaaacgtc tcggcaccac tcaacaagcg
     4861 tatgaaaaag cctttgccgg ggtggtcaat cgcaccctcg acgaagggta tcaggtcatt
     4921 gcgctctcca cttgtacggg catcgacagc tataacaaag acgaccgcat ggtggcgctc
     4981 aacctgcgcc agcacatcag cgatcctgcc cgttaccacg tagtgatgga tgaactcaat
     5041 gatctggaaa tgggcaaaat tcttggagcc tgtcaactca ccgtcggtac gcgtctgcac
     5101 tcagccatta tctcgatgaa tttctccact ccggcaattg ccatcaacta cgaacataaa
     5161 tccgccggga ttatgcagca gctggggcta ccagagatgg cgattgatat ccgtcattta
     5221 ctggacggta gcctgcaagc gatggttgcg gataccttag gccatcttcc ggcgctgaac
     5281 gcacgactta gcgaagccgt cagtcgcgaa cgtcagatgg gaatgcggat ggtgcaatct
     5341 gtgcttgaac gcattgggga ggcgaaatga aggtcggctt ctttttactg aaatttccgc
     5401 tgtcgtcgga aaccttcgtt cttaaccaaa tcactgcgtt tattgatatg ggctttgaag
     5461 tggagattgt cgcgctgcaa aaaggcgaca cccagaacac ccacgcggca tggacgaaat
     5521 acaaccttgc cgccagaacc cgctggttac aggacgaacc tacgggcaaa gtggcgaaac
     5581 tgcgccaccg agccagtcag accttacgcg gcattcatcg taaaaatacc tggcaggcgc
     5641 ttaacctcaa acgctatggt gccgagtcgc ggaacctgat tttgtctgcc atttgcggcc
     5701 aggtcgcaac accgtttcat gccgatgtgt ttatcgctca ttttggccct gctggggtaa
     5761 ccgcggcaaa actacgcgaa ctgggtgtca ttcgcggcaa aattgccact atcttccacg
     5821 gtattgatat ctccagtcgg gaagtgctca accactacac tcccgaatat caacaactgt
     5881 ttcgccgtgg cgacctgatg ttaccaataa gcaatctgtg ggccggaagg ctgcaaaaaa
     5941 tgggctgccc gagggaaaaa atcgccgtat cgcgcatggg cgtggacatg acgcgtttta
     6001 gcccgcgtcc ggtgaaagcg cccgcaacgc cgctggaaat catttccgtc gcacgcttaa
     6061 ccgagaaaaa aggcctgcat gtggcgatcg aagcctgccg tcagttgaaa gagcagggcg
     6121 tggcatttcg ctatcgcatc ctcggcattg gcccgtggga acgacgcctg cgcaccctca
     6181 tcgaacaata tcaactggaa gatgtggtgg agatgccggg ctttaaacca agccacgaag
     6241 tgaaggcgat gctcgacgac gcggatgtct tcctgttgcc ttcggtaacg ggtgcggacg
     6301 gcgatatgga aggcatcccg gtggcgctaa tggaagcgat ggcggtcggc attccggtgg
     6361 tttctactct gcatagcgga ataccggaac tggtggaggc cgataaatcc ggctggctgg
     6421 tgcctgagaa cgatgctcgc gcactggcgc aacgactggc ggcgtttagc caactggaca
     6481 ccgacgaact ggctcctgtc gtcaaacgtg ctcgcgaaaa agtcgaacac gattttaacc
     6541 agcaggtgat taatcgagaa ctcgccagct tgctgcaggc tttatagagg agaacgtatg
     6601 ccatttaaaa cactctcccg acgcaccttc ctgacggcaa gctccgcgct tgccttcctt
     6661 catacccctt tcgctcgcgc actatccgcc cgacaaagcg taaacattaa cgactacaac
     6721 cctcacgact ggatcgcctc atttaaacaa gccttcagcg aagggcaaac ggttgtcgtg
     6781 cctggcggat tggtttgtga caatatcaac accggcatct ttatccctcc cggtaaaacg
     6841 ttacacattc ttggaagctt gcgcggcaac ggcagagggc gatttgtctt acaggacggc
     6901 agccaggtga caggggagga gggcggcagt atgcataaca tcaccctgga tgtgcgcggc
     6961 tctgactgca ccatcaaagg gctggcgatg agcggctttg gtccggtaac gcagatttat
     7021 atcggcggca aaaacaaacg ggtcatgcgc aacctgacca tcgataacct cactgtcagc
     7081 cacgctaatt acgccatctt acgccaggga tttcataatc agattatcgg tgccaacatc
     7141 accaactgta agttcagtga cttacagggc gacgctattg aatggaacgt ggcgattaac
     7201 gaccgtgata ttttgatatc tgaccatgtc atcgagcgca tcaactgtac taacggcaaa
     7261 atcaactggg gcattggcat aggtcttgcg ggaagcactt atgataacaa ctacccggaa
     7321 gaccaggcag tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg tcggcaactg
     7381 atccatgttg aaaatggtaa acattttgtt attcgtaata tcaaagcccg taatatcacg
     7441 ccggatttca gtaagaaagc aggtattgat aacgccacag ttgctattta tggttgtgac
     7501 aactttgtga ttgataatat tgaaatgatt aatagtgccg ggatgttaat cggctatggg
     7561 gtaattaaag gcaaatatct ctcgataccg cagaatttcc gggtgaataa tattcaactg
     7621 gataatactc atcttgctta taaattgcac ggcattcaaa tctccgccgg gaatgctgtc
     7681 tcctttgtgg cgctgactaa cattgagatg aagcgtgcgt cgctggagtt acacaacaaa
     7741 ccgcaacatc tttttatgcg taatatcaat gtgatgcagg aatcctcagt tggacccgca
     7801 ttgagcatga acttcgacat gcgcaaagac gttcgcggcg tgtttatggc gaaaaaagaa
     7861 acactgctgt ctcttgcaaa tgttcatgcg gtgaatgaaa gagggcaaag ctccgtcgat
     7921 atcgacagga ttaatcacca tattgttaat gtggcaaaga ttaactttag attgccggaa
     7981 cggagagagt agatttgcga ccattcctgg aaaaatggag ccatacttag gaacaatgct
     8041 actgcaatcc acaacgaagc ggcgtaacat cacaagtaat tcagtaatca attcagggta
     8101 attgatgctg gcgaaaaaaa tcgaacaagc tataattcag caaccatttt acaggtggat
     8161 gaaataatga cgaatttaaa agcagttatt cctgtagcgg gtcttgggat gcatatgttg
     8221 cctgccacta aggcgattcc caaagagatg ctaccgatcg tcgacaagcc aatgattcag
     8281 tacatcgttg acgagattgt ggctgcaggg atcaaagaaa tcctcctggt aactcacgct
     8341 tccaagaacg cggtcgaaaa ccacttcgac acctcttatg aattagaatc tctccttgaa
     8401 cagcgcgtga agcgtcaact gctggcggaa gtacagtcca tttgcccgcc gggcgtgaca
     8461 attatgaacg tgcgtcaggg cgaaccttta ggtttgggcc actccatttt atgtgcacga
     8521 cctgccattg gtgacaatcc atttgtcgtg gtgctgccag acgttgtgat cgacgacgcc
     8581 agcgccgacc cgctgcgcta caaccttgct gccatgattg cgcgcttcaa cgaaacgggc
     8641 cgcagccagg tgctggcaaa acgtatgccg ggtgacctct ctgaatactc tgtcatccag
     8701 accaaagagc cgctggaccg cgaaggtaaa gtcagccgca ttgttgaatt catcgaaaaa
     8761 ccggatcagc cgcagacgct ggactcagac atcatggccg ttggtcgcta tgtgctttct
     8821 gccgatattt ggccggaact tgaacgcact cagcctggtg catgggggcg tattcagctg
     8881 actgatgcca ttgccgaact ggcgaaaaaa cagtccgttg atgccatgct gatgaccggc
     8941 gacagctacg actgcggtaa aaaaatgggt tatatgcaag cgttcgtgaa gtatggacta
     9001 cgcaacctca aagaaggggc gaagttccgt aaagggattg agaagctgtt aagcgaataa
     9061 tgaaaatctg accggatgta acggttgata agaaaattat aacggcagtg aagattagcg
     9121 gcgaaagtaa tttgttgcga attttcctgc cgttgtttta tataaacaat cagaataaca
     9181 acgacttagc aataggattt tagtcaaagt tttccaggat tttccttgtt tccagagcgg
     9241 attggtaaga caattagcgc ttgaattttt cgagttaagc gcgagtgggt aacgctcgtc
     9301 acatcgtagg aatgcatgca gtgctctggt agctgtaaag ccaggggcgg tagcgtgcat
     9361 taatacctct attaatcaaa ctgagagccg cttatttcac agcatgctct gaagtaatat
     9421 ggaataaatt aagtgaagat acttgttact gggggcgcag gatttattgg ttctgctgta
     9481 gttcgtcaca ttataaataa tacgcaggat agtgttgtta atgtcgataa attaacgtac
     9541 gccggaaacc tggaatcact tgctgatgtt tctgactctg aacgctatgt ttttgaacat
     9601 gcggatattt gcgatgctgc tgcaatggcg cggatttttg ctcagcatca gccggatgca
     9661 gtgatgcacc tggctgctga aagccatgtg gatcgttcaa ttacaggccc tgcggcattt
     9721 attgaaacca atattgttgg tacttatgtc cttttggaag cggctcgcaa ttactggtct
     9781 gctcttgatg gcgacaagaa aaatagcttc cgttttcatc atatttctac tgacgaagtc
     9841 tatggtgatt tgcctcatcc tgacgaagta aataataaag aacaattacc cctctttact
     9901 gagacgacag cttacgcgcc tagtagtcct tattccgcat caaaagcatc cagcgatcat
     9961 ttagtccgcg cgtggaaacg tacctatggt ttaccgacta ttgtgactaa ctgttcgaat
    10021 aactacggtc cttatcactt tccggaaaaa ttgattccac tagtaattct taatgctctg
    10081 gaaggtaagg cattacctat ttatggcaaa ggggatcaaa ttcgtgactg gctgtatgtt
    10141 gaagatcatg cgcgtgcgtt atataccgta gttactgaag gtcaagcggg tgaaacctat
    10201 aacattggcg gacacaacga aaagaaaaac atcgatgttg tgctgactat ttgtgatttg
    10261 ttggacgaga tagtcccgaa agagaaatct tatcgtgagc aaattactta tgttgctgat
    10321 cgcccagggc atgatcgccg ttatgcgatt gatgctgaga agattggtcg cgaattggga
    10381 tggaaaccac aggaaacgtt tgagagtggg attcgtaaaa cggtggaatg gtatttggct
    10441 aatgcaaaat gggttgataa tgtgaaaagt ggtgcctatc aatcgtggat tgaacagaac
    10501 tatgagggcc gccagtaatg aatatcctcc tttttggcaa aacagggcag gtaggttggg
    10561 aactacagcg tgctctggca cctctgggta atttgattgc tcttgatgtt cactccactg
    10621 attactgtgg tgattttagt aaccctgaag gtgtggctga aacagtcaaa agaattcgac
    10681 ctgatgttat tgttaatgct gcggctcaca ccgcagtaga taaggctgag tcagaacccg
    10741 aatttgcaca attactcaat gcgactagcg ttgaatcaat tgcaaaagcg gcaaatgaag
    10801 ttggggcttg ggtaattcat tactcaactg actacgtatt ccctggaaat ggcgacacgc
    10861 catggctgga gatggatgca accgcaccgc taaatgttta cggtgaaacc aagttagctg
    10921 gagaaaaagc attacaagag cattgtgcga agcacctaat tttccgtacc agctgggtct
    10981 atgcaggtaa aggaaataat ttcgccaaaa cgatgttgcg tctggcaaaa gagcgtgaag
    11041 aactagccgt tattaatgat cagtttggtg cgccaacagg tgctgaactg ctggctgatt
    11101 gtacggcaca tgccattcgt gtcgcactga ataaaccgga tgtcgcaggc ttgtaccatt
    11161 tggtagccag tggtaccaca acctggtacg attatgctgc gctggttttt gaagaggcgc
    11221 gcaatgcagg cattcctctt gcactcaaca agctcaacgc agtaccaaca actgcctatc
    11281 ctacaccagc tcgtcgtcca cataactctc gccttaatac agaaaaattt cagcagaatt
    11341 ttgcgcttgt attgcctgac tggcaggttg gtgtgaaacg catgctcaac gaattattta
    11401 cgactacagc aatttaatag tttttgcatc ttgttcgtga tggtggagca agatgaatta
    11461 aaaggaatga tgaaatgaaa acgcgtaaag gtattatttt agcgggtggt tctggtactc
    11521 gtctttatcc tgtgactatg gtcgtcagta aacagctatt acctatatat gataaaccga
    11581 tgatctatta tccgctttct acactgatgt tagcgggtat tcgcgatatt ctgattatta
    11641 gtacgccaca ggatactcct cgttttcaac aactgctggg tgacggtagc cagtggggcc
    11701 tgaatcttca gtacaaagtg caaccgagtc cggatggtct tgcgcaggca tttattatcg
    11761 gtgaagagtt tattggtggt gatgattgtg ctttggtact tggtgataat atcttctacg
    11821 gtcacgacct gcctaagtta atggatgccg ctgttaacaa agaaagtggt gcaacggtat
    11881 ttgcctatca cgttaatgat cctgaacgct atggtgtcgt tgagtttgat aaaaacggta
    11941 cggcgatcag cctggaagaa aaaccgctac aaccaaaaag taattatgcg gtaaccgggc
    12001 tttattttta tgataacgac gttgtcgaaa tggcgaaaaa tcttaagcct tctgcccgcg
    12061 gtgaactgga aattaccgat attaaccgta tctatatgga acaagggcgt ttatctgttg
    12121 ccatgatggg gcgtggttat gcgtggttag acacggggac acatcagagc ctgattgagg
    12181 caagcaactt tattgcaaca attgaagagc gtcaggggct gaaagtttcc tgcccggaag
    12241 aaattgctta ccgtaaaggg tttgttgatg ctgagcaggt gaaagtatta gctgaacctc
    12301 tgaaaaaaaa tgcttatggt cagtatctgc tgaaaatgat taaaggttat taataaaatg
    12361 aacgtaatta aaacagaaat tcctgatgta ctgatttttg aaccgaaagt ttttggtgat
    12421 gagcgtggtt tcttttttga gagctttaac cagaaggttt ttgaggaagc tgtaggccgc
    12481 aaagttgaat ttgttcagga taaccattcg aagtctagta aaggtgtttt acgcgggctg
    12541 cattatcagt tggaacctta tgcacaagga aaattggtgc gttgcgttgt cggtgaagtt
    12601 tttgacgtag ctgttgatat tcgtaaatcg tcatcgactt ttggcaaatg ggttggggtg
    12661 aatttatctg ctgagaataa gcggcaattg tggattcctg agggatttgc acatggtttt
    12721 ttagtgctga gtgagacggc ggagtttttg tataagacga caaattatta tcatcctcag
    12781 agtgatagag gaataaaatg ggatgatcca agtatcaata tttcatggcc agtcgattca
    12841 caagtgctgc tatcagctaa agataataag catcctccat taacaaagat tgaaatgtat
    12901 agttaagatc acgataaatc ttggaagggt tgcaaaattg aataaaatag tgagcaaaag
    12961 tgaaataagg aacgtaatcc acaatgctgg ctatatgatg attactcaga tagctttata
    13021 tgttgcacca ttatttatac tgagttatct gttaaaaaca ctgggggttg cacagtttgg
    13081 taattatgcc ttaatactat caatcgttgc atatttacag attataacgg attatggttt
    13141 ttcttttagt gcaagtcgtg cgatctcaca gaatagagag gacaaagaat atatatcaaa
    13201 aatttatctg tcaactatga ctatcaagtt ggcgatatgc gctttcttat tcttattgct
    13261 catgctattt ttaaatcttt tgcctgtgca agctgaatta aaacaaggaa tattatatgg
    13321 atatcttctt gtaataggaa atactttcca accacaatgg tttttccaag gtatcgaaaa
    13381 attaaaaatc atagcccttt ctaatgttat atcaagatgc gccgcgtgtt tacttgtatt
    13441 tatctatgtg aggaatagcg agg
//
LOCUS       DAOTRK010000124        13287 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.125, whole genome shotgun sequence.
ACCESSION   DAOTRK010000124 DAOTRK010000000
VERSION     DAOTRK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.125"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            72..197
                     /locus_tag="QIE41_003566"
                     /pseudo
     CDS             72..197
                     /locus_tag="QIE41_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            complement(257..763)
                     /locus_tag="QIE41_003567"
     CDS             complement(257..763)
                     /locus_tag="QIE41_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDS3532410.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKEMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(809..1309)
                     /locus_tag="QIE41_003568"
     CDS             complement(809..1309)
                     /locus_tag="QIE41_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDS3532411.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(1395..1574)
                     /locus_tag="QIE41_003569"
     CDS             complement(1395..1574)
                     /locus_tag="QIE41_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDS3532412.1"
                     /translation="MTEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(1955..2761)
                     /gene="trpA"
                     /locus_tag="QIE41_003570"
     CDS             complement(1955..2761)
                     /gene="trpA"
                     /locus_tag="QIE41_003570"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDS3532413.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(2761..3954)
                     /gene="trpB"
                     /locus_tag="QIE41_003571"
     CDS             complement(2761..3954)
                     /gene="trpB"
                     /locus_tag="QIE41_003571"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDS3532414.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(3966..5327)
                     /gene="trpCF"
                     /locus_tag="QIE41_003572"
     CDS             complement(3966..5327)
                     /gene="trpCF"
                     /locus_tag="QIE41_003572"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDS3532415.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEIQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDARAAYDAG
                     AIYGGLIFVTTSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(5328..6923)
                     /gene="trpD"
                     /locus_tag="QIE41_003573"
     CDS             complement(5328..6923)
                     /gene="trpD"
                     /locus_tag="QIE41_003573"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDS3532416.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6923..8485)
                     /gene="trpE"
                     /locus_tag="QIE41_003574"
     CDS             complement(6923..8485)
                     /gene="trpE"
                     /locus_tag="QIE41_003574"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDS3532417.1"
                     /translation="MQTQKPTLELLTCEGTYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(8507..8600)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(8577..8621)
                     /gene="trpL"
                     /locus_tag="QIE41_003575"
     CDS             complement(8577..8621)
                     /gene="trpL"
                     /locus_tag="QIE41_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDS3532418.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            8759..9640
                     /gene="yciV"
                     /locus_tag="QIE41_003576"
     CDS             8759..9640
                     /gene="yciV"
                     /locus_tag="QIE41_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDS3532419.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            9637..10257
                     /locus_tag="QIE41_003577"
     CDS             9637..10257
                     /locus_tag="QIE41_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDS3532420.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10285..12180
                     /locus_tag="QIE41_003578"
     CDS             10285..12180
                     /locus_tag="QIE41_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDS3532421.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QLDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGVPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNKWPF
                     EIYKTRFSLQISNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPALQTTNIFSHLLLPSTSSLLIWFPCLLMVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVVLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAERITPSLTAAVSAPEIARSGVLFSLQTHLSCGAYTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            12391..13266
                     /gene="rluB"
                     /locus_tag="QIE41_003579"
     CDS             12391..13266
                     /gene="rluB"
                     /locus_tag="QIE41_003579"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDS3532422.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
BASE COUNT         3171 a         3290 c         3448 g         3378 t
ORIGIN      
        1 aaaaaggtac aaaaaaacat ctcaaagtct tgttttttgc ttcaaataca attggttatt
       61 gtgttttttg aaacctacca gtgtggtaca tggatattga taccaccgcc aattataaac
      121 tgggcggtgc acagcaacac gatagcgtac gcctcgatcc gtgggtgttt atgttctcag
      181 caggatatcg tttttaattc cgcacaaaaa cgaccccgta atatacgggg tcaataagga
      241 catggtataa agggtattat ttcttcgctt ctacgccatc agtttcagag cgaattaaaa
      301 atttctcagt tgtttgcgga atatgctgaa tcagccagtc ggccatttct ttttcctcat
      361 ttaaaatcgc ttcgatggtt ggaattgaag ctgtatcacc ggcatttttt gctgctgcta
      421 atagtgaggt gtaacaggcg atttcaaatt gctcgaagac atatccgcta atagagcctt
      481 tgactatttc atcagaaggg aatataccac cgattgactg cccaagcgca gccattttac
      541 tcatggaatc tttaatgact gaacgtgaaa tgtcattacg atcaagaata gtttccagtt
      601 gaacaatctg gtttttggtt tcactaagat gttgttcaat acgagcgcgt agttcaggat
      661 aattatctat acgactagcc atggattcaa gcatagattc ggcttgcttt tccattgcgt
      721 gggcgtcacg taaccagtca tgataatgtt caatacgatt catgaaaatg tcctctttat
      781 aaaatgacta aaagttaaag ttcatatttc aggctttatt ttcggctttc ttatttacgt
      841 tattaagtgc cagatcagtc agtttgatgt cggtggcctt ttcttcttca agagtttctt
      901 tcagaagctt cgctgcttta cggtaaccta attgttcagc cagcgtcgct aatgtcccgt
      961 aactggcgat ctcatagtgc tcgactttct gcgctgcggc aatcagtgcg gcatcacgca
     1021 cttcgttttt ctcggtgctt tcgataacct cattagcttc ttcaatgaga ccttccattg
     1081 ccacacattt catgcgctta attttcaggt tcgattcaga ttccacaact tgatcaatac
     1141 gttcaatctg tccatgagtt tcctcgaggt gcgcatgaaa agcctgactt aatttttcgt
     1201 ttgatgttgc tcttgcgagt tttgccagtg cccgggttaa ttgtttttct gcgctgtagg
     1261 tatctgaaag caggtgaata aatacatctt caatggtctt catattcata tttttctcca
     1321 gtgaaatcac tgcgagccat acggcccgca gatgcaatta tctttatcag ctgatattaa
     1381 taattaacgg tgaatcagga tttgcctgat ttattaccac cgctttgttg accgcctttt
     1441 ttacccgctt cagatgcgcg ttgcggatca tttttaaaat taccgccgct atgctgaccg
     1501 cctttacggc cagcgtcgga tgccttttca cggtcttcgg cgaaatttcc tgaaccacca
     1561 cgatgttcgg tcatgttatt tctcccgttg cgttgcattg tttcattaat atgagtgttg
     1621 tgtgtcgaca ctcattaaaa ttagtcgcta atgaaaatta gtcaaattaa gcgcaacgag
     1681 aagaaagagg gaaaatatat tatgaggaac attctggata tattaacaat tgcctgagga
     1741 ataagtgact tagagaaaat tattgacttt actggtgtta tgttgcggga ttaatttgtt
     1801 tgattatatt actgttgggc tgaaaaaaga cgtaagttta cgttcgacag gggtaagcga
     1861 aacggtaaaa agataaataa taaatgaatt taggattttt ccagcttcgt taaagacagt
     1921 taaaatgccg ccagcggaac tggcggctgt gggattagct gcgcgtcgcc gctttcatcg
     1981 gttgtacaaa agctttcagt gccgccagca ttttctctgg ctcattaata tgttgctcga
     2041 tgattttaac gatggccgaa ccagaaatcg cgcccgcagc tcctgcatca atcgctgctt
     2101 ttacctgatc cggggcggaa ataccaaagc cctgcaatgg aggcgcagca ttgtattctt
     2161 tcagcttcgt gaccagatga ttgaggggta acgcggcgcg gttttctgcg ccggtcacgc
     2221 ccgcacgtga caataaatag gtgtatccac gaccgtagga ggctatctgg cgcagcaaat
     2281 catcgtcggc attcggcggg caaataaaga taggtgcgac attatgacgc aacgcggcct
     2341 ggcggaaggg cgcggactct tccactggca catcggcaac cagcaccgaa tcgacgccga
     2401 ctttctcgca ctgggcataa aactcatcaa tgcctttgtt aaacaccagg ttggcataca
     2461 tcaaaaggcc gatgggaatg gtcgggtgct tctggcgaat cagtgccagc atctcaaagc
     2521 actgtgccgg ggtcacacct gccgcaaagg cacgcagtgt ggcgttttga atcgtcgggc
     2581 catccgccag tgggtcggag aaggggatac ctaactccag cgcgtcagca ccggcttcaa
     2641 ttagcgtgtc gataattttc agcgactgct caatgcccgg atcaccgagc gtgacgaaag
     2701 gaacgaatgc gccttctttg cgctccttca actgggcaaa cagagattcg tagcgttcca
     2761 tcagatttcc cctcgagctt tcaaaatatc gtgaacggtg aagatgtctt tatcgccgcg
     2821 accggaaagg ttaaccacca gtagctgctc tttttccggg ttttcgcgca tcattttcag
     2881 cgcatgggcc agggcgtggg aggactccag cgccgggatg atcccttcgt gcaggcagag
     2941 cgttttgaag gcttccagtg cttcatcatc ggtaattgac acgtaatcag cgcgtccagt
     3001 gctgttaaga tacgcgtgtt gtgggccgac agacgggaaa tccagtccgg cggagatgga
     3061 gtaagattct tcaatctgcc cgtcttcggt ttgcatcatc ggcgctttca taccgaaata
     3121 gatgcccaca cgaccatgtt ttaacggtgc gccgtgctcg ccagtttcga taccgtgacc
     3181 gccaggctcc acaccaatca ggccgacgtc ggtttcgttg atgaaatcag caaacatgcc
     3241 gatggcattc gaaccgccgc caacacaggc gataaccgca tccggcaggc gaccttctct
     3301 ttccagaatc tgcgctttcg tttcttcgcc aatcatccgc tgaaactcac gcacaatggt
     3361 cgggtaagga tgcgggccag ccgcggtgcc cagcatatag tgcgcggttt cataactgcc
     3421 ggaccagtcg cgtagcgcct cattacaggc atctttcagc gtcgcggaac cgctatgcac
     3481 cgggatcact tccgcaccca ttaagcgcat acgaaaaacg ttaggcgact ggcgctcgac
     3541 gtctttggca cccatataaa tgcgacattt caggccgagc agggcgctgg caagggccga
     3601 cgccacgcca tgctgaccgg ctccggtttc ggcgatgatt tccgttttac ccatccgctt
     3661 cgccagcaaa gcctggccga gcacctggtt ggttttatgc gcgccgccgt gcagcaaatc
     3721 ttcgcgcttg agatacagcg tggtgttagt gccagccgta atgttctggc atttggtgag
     3781 tgcggttgga cgcccggcat agtttttcag caggtcgttg aactgagcct gaaattcagg
     3841 atctttttgc gcgctgacaa aagcttcttc cagctggcgc agagcaggca tcaggatttg
     3901 tggcacgtac atgccgccaa actcaccaaa atacgggtta agtaatgttg tcattgttcc
     3961 tttccttaat atgcgcgcag cgtctggaaa accgaggcca aaagacgtgc gtctttgatg
     4021 cccggttgcg actctacagc agaattaaaa tcaagtccgg cgcagccggt ttgtgccgct
     4081 tccacgcagt tatctgcgcc taagcccccc gccagcagaa cgttgccaag cgattgaccg
     4141 tttaacagtg accagtcgaa acgttgaccg cttccaccct ggccgttgtc taaaacatat
     4201 ttatcaacgt gctgaagctc gcgggcgggc agggtttcac cgacgcttaa tgctttccag
     4261 atggcaacgt gtgctggcag agcttcacgc agcgtatcga tatacagctg atcttcatta
     4321 ccatgcagtt gcactgccgc cagcgataac accttagctt tgtccaccac atcggcaata
     4381 tcgtgattgc ggaacacgcc aacatactgc aacggtgccg cagccatcac ttcctgcgcc
     4441 tgttcaacgt tgacgcaacg cggtgacgtt gtaacaaaaa tcaacccacc gtaaatcgcg
     4501 cccgcgtcat aagcggctct agcatcttgc ccacgcgtca ggccacatac tttattctca
     4561 cccagcaaca cccggcgcac ggcggcgttc aaatcgtcat gggccatcaa cgccgaacca
     4621 atcagaaaac cgttagcgaa gtggcttaac tcgcgcacct gagcgtaagt attgatgccg
     4681 gattcgctga ttaccgtcac gttgtgcccc agtttcggcg caagctcgcg ggtacggttg
     4741 agatcaatcg acaaatcacg tagatcgcgg ttgttgatgc caacgacctt tgcccccaat
     4801 gcaatggcgc gctccagttc ctcttcatta ctgacttcgg taagcacgcc catctccaga
     4861 ctgtgggcga cggcggcaag gtggcgatat tgttcgtcat ccagtactga aagcattaat
     4921 aagcaggcat cggcctggta atagcgcgcc agatagatct ggtaagggtc gataatgaag
     4981 tctttacata aaatcggctg cggggcgatt tggctgacga ttgggaggaa atcaaagctc
     5041 ccctgaaaat atttctcatc agtcagcacc gaaattgccg aagcgtaatg tttataaatg
     5101 gcggcaatgc gtgccggatc gaaatcatca cggatcacgc cttttgacgg cgaggctttc
     5161 ttgcactcaa gaataaacgc cgtgcgtgca ccctgtagcg catcataaaa atgtcgcgtg
     5221 ctcggctgaa tctcattctg aaaactggcc agcggttgct gctgtttgcg ggtttctacc
     5281 caaatcgcct tgtctgcgac gattttcgct aaaacggttt gcatcattta ccctcgtgcc
     5341 gccagtgcgg tgactctgtc gtaagcggaa ccactgcgca gtacctcaag aacagtttgc
     5401 gcattggctt gcagatcttc atggccatgc aggcgcatta acatggcgac attcgccgcg
     5461 acggctgctt catgggcggc gtcgccttta ccttgtaaca agcgtgttaa aatgtcacgg
     5521 ttttcttccg gtgttccacc tgccagttgc tcctggtggt agggggtcag gccaaaatct
     5581 tcagcggtca attgatagct cttaatttcg ccgtcatgca gttcggcaac gattgtcggc
     5641 gcgtgtaatg aaacttcatc catcccgccg ctgtgcacca ctgccgcgcg ttgatacccc
     5701 agcacgcgca aggtttcggc aatcggtagc accagttccg gactataaac accaattaac
     5761 gccagcggag gatgcgccgg gttaatcagt ggccccagca cgttgaacag ggtgcgggtt
     5821 ttcagttgct ggcgaaccgg catcgcgtgg cgaaatccgg tgtgatattt cggcgcaaag
     5881 aggaaacaga cgcctaactc atccagcgcc tggcgcgatt tatcggcgtt catatcaaga
     5941 ttaataccga atgccgccag cagatccgac gaaccggatt tactggagac actacggttg
     6001 ccgtgtttcg ccactttcag cccacaggcc gcggcgacaa acgcactggc ggtagaaata
     6061 ttgatactgt tgctgccgtc accaccggtg ccgacgatat cggcaaacag ataatccggg
     6121 cgcgggaatg gcgcagcatt ttccagaagg gcagttgctg ccccggcgat ttcgttcggg
     6181 tgctcgccgc gaattttcat gctcaccagc gccgccgcga gctgttctgg cttcagctcg
     6241 ccacgcacca ccgctgaaaa cagctggtgg ctttcttgct ggctaagcgt ctgcgcctga
     6301 tacagttttt ccagaatcgg ttgcagcgtg ttagtcggtt ccagtttctg ctgcgcccag
     6361 gccaatgttt gttccagcag gcgagcgccc tgggtagtaa gaatggattc cggatggaac
     6421 tggaatccac aaacgcgatc tgcatcgtga cgcaccgcca tcaccatgcc attaaaatgg
     6481 gcgttgatgg ttaaaccggc cggaatgtta ctgccaacca gcgagtgata acgcgccact
     6541 ggcagcgggt ttgttaatcc ggcaaacatc gcctgaccgt catgttcaat gctggaggct
     6601 ttaccgtgaa gaatttcgcc cgcttgaccg acataccccc cataagcttc gacaatcgcc
     6661 tgatgaccga ggcaaatgcc aataattggc agcttgccgc gcaggcgggt gaggagttcc
     6721 ggcatacaac cggcttcact tggcacaccg gggccaggag aaagcatcag caccggattg
     6781 ctcattgtcg ccaggcgttc aattaaggtc tgcgccggaa tatggttgcg gtaaatcacc
     6841 acgttatgac cattgctgcg caactgatct gccaggttgt aagtaaaaga gtcgatatta
     6901 tcgagcagca gaatgtcagc catcagaacg tctcctgtgc atgatgcgcg gtggcaatag
     6961 cgcgcagtac agcgcgggct ttattacggg tttcgtcggc ttccgactgc ggaacagaat
     7021 caagtaccac gccagcaccg gcttgcacgg tggcgatacc gttttccacc agcgccgagc
     7081 ggatcacaat gcaggtgtcg agatcgccat gcgcggtgaa ataacctacc gcgccgccgt
     7141 agctgccgcg gcgacgacct tccgcctcgg caattaactg catagcgcgt actttcggcg
     7201 caccgcttaa cgtccccata ttcatacagg cgcggtaagc gtgcagggcg tcgagatcgt
     7261 ggcgcagctc accgaccacg cgggagacca ggtgcatcac gtaagagtaa cggtcaactt
     7321 tggtgagatc ggcgacgtag cggctgccgg gggtgcaaat gcgtgccaga tcattacggg
     7381 cgagatccac cagcatcaga tgttcagaaa gctctttatg atcggtacgc atttccagtt
     7441 cgatgcggct gtcgaggtct ctgtccagcg agccatcggc gcgacgaccg cgcgggcgtg
     7501 ttccggcaat cgggtagatc tcaatctggc ggctggtggc gtcatacttg agcgaacttt
     7561 ccggcgacgc gccaaacagg gtgaaatcat tatcctgcat aaaaaacatg tacgggctgg
     7621 gattactctt tttcagcacg taataggccg ccagcggtga cgggcagggc agagagaaac
     7681 ggcgagatgg caccacctgg aaaatttctc cagcgcgaat cgctttttgc aacaaacgca
     7741 ctacgccacc gaactcttca tcgctctgat tacattcaca acgcatatgc ggcacggaaa
     7801 ccaccggcag cggtggcgcg gcttcggtca gctgctggcg aagatcgttc aggcgagcag
     7861 tgagacgttg tttttcttct tcattcggag caaacaggct ggcctgaatg cgggtgcttt
     7921 ttttctgatg gtcaatcacc atcagcgttt ctgcgagata aaagcagaaa tcaggacagt
     7981 tattttccgc tgccagttgc ggtaaatctt caaatcccgc cacaaggtca taagagaaca
     8041 ggccgccgaa gaacatggct tctcgttctt ccttcggtac attcaacaga ttctgcaata
     8101 aacggaaagc gtcaaaaaca gaaagggagc ataagcgggc gtcttcatcc agcagtggac
     8161 tgacaggggg gaagcgcagc acacggcagt ttggtgattg ttcactttcc acacccgcag
     8221 gcagggcgtt atccagtagt gccagcaggg cttcgccatt gccggaaagt gcctgaattg
     8281 tgacagtgtc acctaatgct gtaatgcgca gcgcactgtc tatcagcagc aggcttttta
     8341 aatcatcttt gctgtcgata tctgcggatt ccagcagcag cgttgctgga cgattcccac
     8401 acaactggtg aaaaagcgca gtcgggttgt cgcgataagt gccttcgcag gttagcagtt
     8461 cgagagtcgg tttttgtgtt tgcattgtta ttctctaatt ttgttcaaaa aaaagcccgc
     8521 tcattaggcg ggctgggtat ctgattgctt tacgcatggt gaatacactg cccgtttcag
     8581 gaagtgcgcc accaaccttt cagtacgaaa attgctttca ttgtcgatac cctttttacg
     8641 tgaacttgcg tactagttaa ctagttcgat gattaattgt caacagctca tttcagaata
     8701 tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt
     8761 gagcgacacg aattacgcag tgatttacga cctgcacagc cataccacag cttccgatgg
     8821 ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgag atgcgcgtcg gcaccctggc
     8881 gatcaccgac catgacacca cagctgcaat ctcaccagca agagaggaaa tttcacgttc
     8941 gggactggcg ctgaatctta ttcccggcgt ggaaatttcc acggtctggg aaaatcatga
     9001 aattcatatt gtcgggctga atattgatat tactcatccg ctaatgtgtg agttcctcgc
     9061 gcagcagaca gaacggcgca atcagcgggc gcagctgatt gctgaaagac ttgaaaaagc
     9121 gcaaatacct ggggcgctgg aaggcgcgca acgactggcg cagggcggtg cggtgacgcg
     9181 cggtcatttt gcgcgtttcc ttgtggagtg cggcaaagcc agttcaatgg cggatgtctt
     9241 taaaaaatat ctggcgcgcg ggaaaaccgg atacgttccg ccacagtggt gtacaataga
     9301 acaagctatt gatgtcattc atcattctgg cggtaaggcg gtgctagctc atcctgggcg
     9361 gtacaatctt agtgctaaat ggctgaaaag attggttgcg cattttgccg aacaccacgg
     9421 cgacgcgatg gaagtcgcgc agtgccagca atcgcccaat gaacgcaccc agctggccac
     9481 acttgcgcgt cagcatcatt tatgggcatc acaaggatct gattttcatc agccgtgccc
     9541 gtggattgaa ctgggtcgta aactctggct gcctgcaggc gttgaaggcg tctggcagct
     9601 atgggaacag ccgcagaaca ccacagagag ggaattatga gccagttttt ttatattcat
     9661 cctgataacc cgcagcaacg tctgatcaac caggcggtgg agatcgtgcg taaaggcggg
     9721 gtgattgttt atccaactga ttccggctat gcgctcggct gtaaaattga agataaaaac
     9781 gccatggagc gtatttgtcg tattcgccag ctgccggacg gtcacaactt taccctgatg
     9841 tgtcgcgatc tttctgagct ctcgacctat tcattcgtgg ataacgttgc gtttcgttta
     9901 atgaaaaaca acacgccggg caactacacc ttcatcctga aagggacgaa ggaggtgcca
     9961 cgccgtctgt tgcaggaaaa acgcaaaacc atcggtatgc gtgtgccttc taacccgatt
    10021 gcgcaggcgc tgcttgaagc attgggcgaa ccgatgcttt ccacatcact gatgctgcca
    10081 ggtagcgaat ttaccgaatc cgatccggaa gaaattaaag accgtctgga aaaacaggta
    10141 gatttgatta ttcacggcgg ctatctcggg cagaaaccaa caacggttat tgatcttact
    10201 gatgatacgc cggtcgtggt gcgtgaaggc gtaggtgatg tgaagccttt cttataacaa
    10261 attgcgttcc acggatggaa gactatggca gggaagtttc gctgcatttt gctgttgata
    10321 gcagggctgt ttgtatcatc tctaagttat gcagaaaaca cggagatccc ttcttatgaa
    10381 gaagggatct cgctctttga tgttgaagcc accctgcaac tggatggggt gctcgacatc
    10441 aaagagaaca ttcattttca ggcgcgaaat cagcagatta agcacggatt ttatcgtgat
    10501 ttaccacgac tatggatgca gcctgatggt gacgctgcac tgctgaacta tcatattgtt
    10561 ggcgtcaccc gtgatggtgt tcctgaaccc tggcatcttg actggcatat cggtttaatg
    10621 agtattgtcg tgggcgataa acaacgtttc ttgcctcaag gtgactatca ttatcaaatt
    10681 cattatcagg ttaaaaatgc tttcctgcgt gagggagatt ctgatctgtt aatctggaac
    10741 gtgaccggta acaagtggcc gtttgaaatt tataagaccc gattttctct ccagatctct
    10801 aatattgcgg gtaatccatt tagcgaaatt gatctcttta ccggagaaga gggcgacaca
    10861 tatcgaaatg gccgcatcct tgaggacgga agaattgaat cccgcgatcc gttttatcgt
    10921 gaagacttca cggtcctcta tcgctggcct cacgctttac ttagcaatgc cccggctcta
    10981 caaacgacga atattttcag ccatcttctt ttaccctcca cgtcatcgtt gttaatttgg
    11041 tttccgtgtc tgctcatggt ttgtggatgg ttatatctct ggaagcgcag gccgcaattt
    11101 acgccggtag atgtgattga aaccgatgtc attccgccag attacacgcc cggcatgtta
    11161 cgtctcgatg cgaagctggt ttacgacgat aaaggttttt gtgccgatat cgtaaatctg
    11221 attgtgaaag gcaaaattca tctggaagat cagtatgaca agaaccagca aatcctgatt
    11281 cgtgttaatg aaggcgcgac cagaaataat gcggtattac tgcccgcaga gcagttatta
    11341 ctggaagcgt tatttcgtaa aggcgataag gtcgttctta cggggagacg caacagagtg
    11401 ttacgcaggg catttttacg gatgcagaaa ttttatctgc cgcgtaaaaa gtcttcgttt
    11461 taccgacctg atgcgttttt gcaatggggc ggaatggcaa tattggcggt cattctctac
    11521 ggtaacctga gtcccgtagg ctgggcagga atgagtctgg tgggcgatat gtttattatg
    11581 atctgctggc ttcttccttt tttattttgt tcccttgagc ttttgtttgc ccgcgatgat
    11641 gacaagcctt gcgttaatcg tgtaatcatc actttgtttt taccactgat ttgttcaggt
    11701 gtggccttct attctctcta tatcaatgtc ggagatgtat tcttttactg gtatatgcca
    11761 gcgggttatt ttagcgctgt tgtcctgacc ggttatctca ctggcatggg gtatattttt
    11821 ctgccaaagt ttacccaaac tgggcagcaa cgttatgccc acggtgaagc tatcgttaac
    11881 tatcttgcgc gtaaagaggc ggcaacacac agtgggcggc ggcggaaagg ggaaacacgg
    11941 aaactggatt acgcgttgtt aggttgggcg gtctcggcaa accttggtag agaatgggca
    12001 gaacgtatca ccccatcact cacagcggct gttagcgccc cggaaattgc ccgtagtggc
    12061 gttttattct cattgcagac gcacctgagt tgcggggcct ataccagttt gttggggcga
    12121 agctattccg gtggtggtgc tggcggcggt gcgggtggcg gaggcggtgg tggttggtaa
    12181 ccaaatcgac gttaatcgaa acaagcttta accataaaga tagtgtagta tgttgcgcct
    12241 caaagccagg ccgataaacg tcgagtcgtt tacttaaggc ctgaagagtt caaacagact
    12301 ttactatcag tgagtcagat gtcagttctg actcactgtt gcacaaaagc atatcagacg
    12361 cctgggaagg cgacaccaaa aggaagctct atgagcgaaa agctacagaa agtgctggcg
    12421 cgtgccggcc acggttctcg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt
    12481 gttgatggca aaattgctaa actcggcgat cgcgttgaag ttacccctgg attgaaaatc
    12541 cgtatcgatg gtcaccttat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg
    12601 gcctattaca aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccaacg
    12661 gtgtttgacc gtctgccaaa gctgcgcggt gcgcgctgga ttgccgtggg tcgtctggac
    12721 gttaatacct gcggtctgct gctgtttacc accgacggtg aactggcaaa ccgcttaatg
    12781 catccaagcc gtgaagttga acgtgaatat gccgtgcgtg tatttggtca ggttgacgac
    12841 gcgaaactgc gtgatttgag tcgtggcgtg cagttggaag atggtccggc agcctttaaa
    12901 accatcaagt tcagcggcgg cgaagggatc aaccagtggt acaacgttac tctgaccgaa
    12961 ggccgtaacc gtgaagttcg tcgtctttgg gaagcggttg gtgtgcaggt aagccgcctg
    13021 atccgtgttc gttacggtga tatcccactg ccgaaaggtc tgccacgcgg tggctggacg
    13081 gagctggacc tcgcccagac taactatctg cgcgaactgg tggagctacc gccagaaacc
    13141 agctctaaag tcgctgtaga aaaagaccgt cgtcgtatga aggcgaatca gattcgtcga
    13201 gcggttaagc gtcacagtca ggtgagcggc ggtcgtcgtt ctggtggacg taataataac
    13261 ggttaattta agataagtat aaaataa
//
LOCUS       DAOTRK010000125        13245 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.126, whole genome shotgun sequence.
ACCESSION   DAOTRK010000125 DAOTRK010000000
VERSION     DAOTRK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.126"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            137..1636
                     /gene="pitB"
                     /locus_tag="QIE41_003580"
     CDS             137..1636
                     /gene="pitB"
                     /locus_tag="QIE41_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDS3532423.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1685..2377)
                     /locus_tag="QIE41_003581"
     CDS             complement(1685..2377)
                     /locus_tag="QIE41_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDS3532424.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLERKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2551..3264
                     /locus_tag="QIE41_003582"
     CDS             2551..3264
                     /locus_tag="QIE41_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDS3532425.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3296..4054
                     /locus_tag="QIE41_003583"
     CDS             3296..4054
                     /locus_tag="QIE41_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532426.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4100..5449
                     /locus_tag="QIE41_003584"
     CDS             4100..5449
                     /locus_tag="QIE41_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDS3532427.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVSLAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVFSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5449..6273
                     /locus_tag="QIE41_003585"
     CDS             5449..6273
                     /locus_tag="QIE41_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532428.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKKYKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPL"
     gene            6285..6845
                     /locus_tag="QIE41_003586"
     CDS             6285..6845
                     /locus_tag="QIE41_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDS3532429.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKYGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6876..7946
                     /gene="lptF"
                     /locus_tag="QIE41_003587"
     CDS             6876..7946
                     /gene="lptF"
                     /locus_tag="QIE41_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDS3532430.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7862..8890
                     /gene="lptG"
                     /locus_tag="QIE41_003588"
     CDS             7862..8890
                     /gene="lptG"
                     /locus_tag="QIE41_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDS3532431.1"
                     /translation="MVSTRTHANGPADAGRTRLLFAAEIFPMNVFSRYLIRHLFLGFA
                     AAAGLLLPLFTTFNLINELDDVSPGGYRWTQAVLVVLMTLPRTLVELSPFIALLGGIV
                     GLGQLSKNSELTAIRSTGFSIFRIALVALVAGILWTVSLGAIDEWVASPLQQQALQIK
                     STATALGEDDDITGNMLWARRGNEFVTVKSLNEQGQPVGVEIFHYRDDLSLESYIYAR
                     SATIEDDKTWILHGVNHKKWLNGKETLETLDNLAWQSAFTSMNLEELSMPGNTFSVRQ
                     LNHYIHYLQETGQPSSEYRLALWEKLGQPILTLAMILLAVPFTFSAPRSPGMGSRLAV
                     GVIVGLLT"
     gene            complement(9057..10229)
                     /locus_tag="QIE41_003589"
     CDS             complement(9057..10229)
                     /locus_tag="QIE41_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDS3532432.1"
                     /translation="MGLYDKFARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10229..10477)
                     /locus_tag="QIE41_003590"
     CDS             complement(10229..10477)
                     /locus_tag="QIE41_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDS3532433.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10509..13163)
                     /locus_tag="QIE41_003591"
     CDS             complement(10509..13163)
                     /locus_tag="QIE41_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDS3532434.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFSCQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPDAETRAVSTFVNCGKALPEH
                     GIEIRNEAGIPVAERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHARAAYPRAGSTDPKRIWRDRGYRSVAAPQYSPNVLRQACPCGSEKTLS
                     EGLCCKPSCAGIPGMNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAADARCGSVRMVGIPLSVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
BASE COUNT         3187 a         3267 c         3450 g         3341 t
ORIGIN      
        1 gaatgaatgc ttattatctg atacacaaga aataacactc tttttatcgt taaaaaatga
       61 tatttcactt tgcccacgcc gttaaagtca ctgataatgc gtccgttcgt aaattcaaaa
      121 tggcgtaatc taatatatgc taaatttatt tgttggcctt gatatataca cggggctttt
      181 gttattactt gctctggcat ttgtgttgtt ctacgaagca atcaatggtt ttcatgatac
      241 ggcgaatgcg gtggcaaccg ttatttatac tcgtgccatg caaccacaac ttgctgtggt
      301 gatggcggca ttttttaact tttttggcgt attgctaggc ggacttagcg ttgcctatgc
      361 cattgtccat atgttgccaa ccgatttgtt gctgaatatg gggtcaactc acggcctggc
      421 gatggtcttt tccatgctgc tggcggcgat tatctggaac ctgggaacgt ggttctttgg
      481 tttaccggcc tccagttcgc ataccttgat tggtgcgatt atcggcatcg gtttaaccaa
      541 cgcgctgtta accggctcat cggtgatgga tgcgttaaac ctgcgtgaag tgaccaaaat
      601 tttctcctcg ctgattgttt cccctatcgt cggcctggtc attgcgggag gcctgatatt
      661 cctgctgcgc cgctactgga gcgggacgaa aaagcgtgac cgtattcacc gcattccgga
      721 agatcgcaaa aagaaaaaag gcaaacgtaa gccaccattc tggacgcgta ttgcgctgat
      781 tgtttccgct gcgggcgtgg cgttttcgca cggtgctaac gacgggcaga aagggatcgg
      841 cctggtgatg ctggtactgg tggggattgc ccctgctggc ttcgtcgtca atatgaacgc
      901 gtccggctat gaaattaccc gtacccgcga tgccgtcacc aacttcgaac actacctgca
      961 acagcatcct gaactgccgc agaagttgat tgcgatggaa cctccattgc ctgcggcatc
     1021 gactgatggc acgcaagtaa cggagtttca ctgtcatccg gcaaatacct ttgatgcgat
     1081 tgcgcgcgtt aaaacgatgc tgccaggcaa tatggaaagt tacgagccgt taagcgtgag
     1141 tcagcgcagc cagctgcgcc gcattatgct gtgcatctct gatacctccg cgaagctggc
     1201 gaaactgcca ggcgtcagta aagaagacca gaacctgctg aaaaaactgc gcagcgatat
     1261 gttaagcacc attgagtacg ctccggtgtg gatcatcatg gcagtagctc tggcgctcgg
     1321 cattggcacc atgattggct ggcgtcgtgt agcgatgacc atcggtgaga agattggtaa
     1381 gcgcggcatg acgtatgcgc aaggcatggc ggcacaaatg acggcggcag tgtctatcgg
     1441 ccttgccagt tatattggga tgcccgtctc cacaacacac gtcctctcgt ctgcggtagc
     1501 agggacgatg gtggtggacg gcggtgggtt acagcgtaaa acggtaacca gcatcctgat
     1561 ggcgtgggtg tttactttac cggcggcgat ttttctttct ggtgggttgt actggatagc
     1621 attgcagttg atttaacctt cctgaaaatg cccggtcctg gcgatcgggc atttccattt
     1681 ttgactagtg ataaccacgc gcggtcataa aatccgtaat cgctttttcc gcatcaacca
     1741 gcacttgttc cagcggttga ttggcatcga tatcaaccag ttgcgcgcct ccaaaggtta
     1801 actgtggcgt tatggctatc ttcctttcca gcgattctct tttgtggtca ggcttacgcg
     1861 cgcaggccac gtccagatcg acattgagtt tgatgaccag atcgggctta tggctcgcca
     1921 tccagtgaaa cgctttacgt tcctggcttg ccagccatga gacaaaacga ccaccttcaa
     1981 cgttaggtgg gaacaccgta ccatcgtaag caccaggaat ttggtcctga ggataacggt
     2041 cggttagaac aattaacccg cgacgacgac aggcaagcat atgacgaaag cgcagtaagc
     2101 gacgggcgac aaacgctgta attaccagcg ccggaacagg gccaggcaat ttttttgctg
     2161 ttttcacctg atttcgttca attgttttat gtaaggattt tcccatcaac ggtaattttg
     2221 tcactgcacg accgacattt ccggcctgtt ttcctaaatg aactctttcg gcagcaccat
     2281 atttttcgac aacggtaatg agatgttcac acaccgttga cttgcctgaa ccatcgctac
     2341 caataacggc aattaatgga ggtgttattg actgcatgaa tatatccttt aatttaaata
     2401 tccattaaaa atatttattt ggttaatatg tttttatgaa agcgtaattc aggtcaatgt
     2461 cacaattaac cattgtcaca ataaggttga acggatattc tgagcacgga ccacgtaata
     2521 ttcaatacat tatttgctgc cgggtttttc gtgaatcaac gtaatatgtc aataattaat
     2581 tccacgccag tgcgtgttat tgccattgta ggatgtgatg gttcaggtaa atcgaccctc
     2641 acggcaagcc tggtaaatga actggcagca agaatgccaa cagaacacat ttatctcggg
     2701 caatcgtccg ggcgaattgg cgaatggatt tcacagctcc ctgttattgg cgcacctttt
     2761 gggcgttatc tgcgaagtaa agcggcacat gtgcacgaaa agccctcaac accgcctggc
     2821 aatattactg cgctggttat ctatctgctt tcctgctggc gggcgtataa gtttcgcaaa
     2881 atgttgtgta aaagccagca aggctatctg ctcatcaccg accgctaccc gcaggttgaa
     2941 gtgccgggtt ttcgctttga tggcccgcaa ttggcaaaaa ccacgggcgg taacggttgg
     3001 ataaaaatgt taaggcagcg cgagctgaag ctgtaccaat ggatggcgtc ttatttgccc
     3061 gtattgttga ttcgtcttgg cattgatgaa caaaccgcgt ttgcgcgtaa acctgaccac
     3121 caactggcag cgttacagga aaaaatcgcc gttacgccgc aactgacatt caatggcgca
     3181 aagatccttg aactggatgg tcggcaacca gccgatgaaa ttatgcaagc gtcactacgc
     3241 gcaattcacg ccgcgctttc ctgatcacca aaatggatag gggatgattt aacgaatgga
     3301 tgcactacaa acacaaactg ttaatagtac aaccgcgccg cagcccaact acattccggg
     3361 gctgattgcg gtggtcgggt gtgatggcac cggtaaatcc acgctgacca ccgacctggt
     3421 gaaatcgctg caacaacact ggcaaaccga gcgccgctat ttggggctgc tctccggcga
     3481 agacggcgac aaaatcaaac gattgccgtt ggttggcgtc tggctcgaac ggcgactggc
     3541 ggccaaatcc tcgaaaaccc aaagcatgaa aaccaaatct ccagcgctat gggcggcggt
     3601 gattatgtac tgcttctcgc tgcgaagaat ggcgaatcta cgcaaggttc agcgactggc
     3661 gcaaagtggc gttctggtgg tcagcgatcg cttcccgcag gctgaaattt cgggctttta
     3721 ttatgacgga ccggggatcg gcgtcgaacg tgcgaccggg aaaatcagca tgtttctggc
     3781 gcagcgcgaa cggcgtttat accaacaaat ggcgcaatac cgtcctgaat taatcattcg
     3841 tttggggatt gatattgata ctgccatctc ccgcaagcct gaccatgact atgccgagct
     3901 gcaggacaaa atcggcgtca tgtcgacgat tggctataac ggcacaaaaa ttcttgagat
     3961 tgattccaga gcgccctaca gcgaagtgct ggaacaggca cagaaggcgg tttctctggt
     4021 tgccatcgtt tctgaccgcc gaagtttaac ttaaggctgg cgggatactt tttactatca
     4081 acgataaggt caggctgaat tggcgggttt taacatcaaa cattggtttg cagatggcgc
     4141 gtttcgcacc attattcgca atagcgcctg gttaggctcc agtaatgtcg tgagcgcttt
     4201 gctgggtctg ttggcgctct cgtgtgccgg taaagggatg acgcccgcca tgtttggcgt
     4261 actggtgatt gtgcaatcgt acgccaagtc gatcagcgat tttattaagt ttcagacatg
     4321 gcagttggtg gtgcagtacg gaacgccagc cttgaccaac aataatccgc agcaatttcg
     4381 caatgttgtc tcattttcct tctcgctgga tatcgtcagc ggcgcggtgg cgattgtcgg
     4441 cggcattgcc ttactgccat tcctttccca ttcattaggt ctggatgacc aaagcttttg
     4501 gctggcggcg ctctattgca cgctcattcc ttcaatggct tcctccacgc cgaccggcat
     4561 tctgcgtgcg gtagatcgct ttgatttgat tgctgtacag caggcgacga aaccttttct
     4621 gcgcgcagcg gggagcgtcg tagcctggta ttttgacttt ggttttgcgg gttttgttat
     4681 tgcctggtac gtgtcgaatc tggttggcgg caccatgtac tggtggtttg ccgcgcgcga
     4741 attacgccgc agaaatatcc ataacgcctt caaattgaat ctgtttgagt ctgcccgaca
     4801 catcaaaggc gcgtggagtt ttgtctggtc aaccaacatt gcccattcca tctggtcggc
     4861 gcgtaattcg tgcagcacag tgttggtggg gatcgtgtta ggacccgctg ccgccgggtt
     4921 atttaaaatc gccatgacat tcttcgacgc cgccggaacg ccagcgggtt tactgggtaa
     4981 aagtttttac ccagaggtga tgcgtttaga tccgcgcacc accagaccgt ggttgttagg
     5041 cgtgaagtcc ggtttactgg cgggcggaat cggtattctg gtgtcgctcg cggtgttgat
     5101 cgtcggcaag ccgctcattt cgctggtgtt tggcgttaag tatctcgaag cgtatgacct
     5161 gattcaggtg atgctgggcg caattgtgat ttcgatgctg ggcttcccac aggagtcatt
     5221 gctattgatg gcgggaaaac agcgcgcttt tctcgtggcg caaaccatcg cctcaatcgg
     5281 ctacatcgta ttgctgttca tgttctgtca tctatttggc gtgctggggg ctgcatttgc
     5341 ttacttcggt gggcaatgtc tggatgtggt gttctcgcta attccgacct taaaggcatt
     5401 ttttcagcgc cattccttgc tttataacgc agccggagag aaatcctgat gatgagaaaa
     5461 tatttcccgc ttgaggcgag cgagcgtcta tttgttgcga ttgaagaaga tgatgtcgtt
     5521 gatgcacagg tcagtttgcc gccgactatc gcactaagtt gtaccaccga aatcatccat
     5581 gataattacg cgttatgcct gaaattctgg ctggatggtg ttaatcgtca ggagctactg
     5641 cgtttaatcc gcaaacaggc gaaaggcgat gagctgacga cagacgagcg caaaaagtat
     5701 aaatacatgc gagcgcgcta caagcatctg cgctttgcgc aacggctgta tttaaagaag
     5761 catcaggccg gatttttatt cggcaaaacg accgtttttt tggggcgttt tcaggacggc
     5821 tttcgtaacg gtaagaaaaa tattgtgtcg tattacggca acctgttacg ggtttattta
     5881 agctctccgg tctggtcgtt ggtgaactat tcattacgcc atagccagtt agaaagcgta
     5941 agcggtttta ttgcctatcg gcaaaagcaa atgcataccc tgaaagagat aattgccaaa
     6001 ccgcgactaa ctggcaggga atttcatgat gtgcgaaaaa tcatcagcca gcaggtttct
     6061 tattacgaca ccctaagatc gcttgatcct gaaaataaag aggcgttaca gatttcccgc
     6121 ttcctcgccg ccattaatgg cctgatgggg gataaacacg acgacatggt ggcggatgat
     6181 atggaaaacc gccagtcgta tgatgcaccg atggcgctgg atagcgatat tcgtcagcgc
     6241 ttagaactgc tgatttcgcg ttttccgcta taaccggagc aatgatgtca tctcgtttga
     6301 ttattgcgct gattatcatg ctcttagccc cgggtgtgca ggcgcacaat ttcgtcactg
     6361 gtaaaacagt tacccctgtt tatatccagg aaggcggcga gctgcttctg aatagcgacg
     6421 acgaaattca ctaccaaaag tggaacagca cacagttggc gggaaaagtc cgcattattc
     6481 agtatatcgc cgggcgtaag tcggcgaaga aaaagaactc actgttaatt aaagcggttg
     6541 aagcggcaaa ctttccgcag gatcgttttc agcccaccac cattgtgaac accgatgatg
     6601 ccatctttgg taccggctat tttgtcgttg gcaaaatcga aaaaaataaa cgccgctatc
     6661 catgggcgca attcgttatt gacggcaatg gacagggacg tgttgcctgg cacttgcagg
     6721 agcagagttc aaccattctg gtgctgaata aatacgggca aattcagtgg gcaaaggacg
     6781 gcagcctgac gccggaagag gtagatcatg tcattgcgct ggcgcagaaa ctgatcaatg
     6841 aataaaaaat tcaaaggatt gagggcgtta gcgagatgaa gctggttgaa cactacatca
     6901 tgcgtggtac gcgccgtctg gtgttgatta tcgtcggttt tctgatcttt attttcgcca
     6961 gctactccgc acagcgttat ctgaccgaag cggcaaatgg gacattagct cttgatgttg
     7021 tgctggatat cgttttctac aaagtgctga ttgcactgga gatgttgtta cctgttggac
     7081 tgtatgtgtc agttggcgtg acgctagggc agatgtatac cgattcggaa attaccgcta
     7141 tttctgcggc ggggggcagt ccgggacgct tgtacaaagc cgttctttat ctggcgatac
     7201 cgctaagtat ttttgtcacc cttctgtcga tgtatggtcg tccgtgggct tatgcgcaga
     7261 tttatcaact ggagcaacag tcacagtcgg agctggatgt tcgccagttg cgggcaaaga
     7321 aatttaacac taacgataac ggacgaatga tcctttcgca gacggttgat caggataata
     7381 atcgcctgac tgacgcgctg atttatactt ctactgccaa tcgaacccgc attttccgcg
     7441 cccgttcggt tgatgtggtt gacccatcac ctgagaaacc gaccgttatg ttgcataacg
     7501 ggaccgccta tcttctcgat catcaggggc gtgacgacaa cgaacagatc taccgtaatc
     7561 tgcaattaca tctgaatccg ctggatcaaa gccctaacgt caaacgcaaa gcaaaatcgg
     7621 tcacggagct ggcgcgctcc gcctttcctg ccgatcatgc cgaactgcaa tggcgacaaa
     7681 gccgtggcct gacagcgttg ttgatggcgc tgctggccat ttcattaagt cgggtaaaac
     7741 cgcggcaagg gcgattttca acgttattgc cactgacgtt gctgtttgtt gccatttttt
     7801 atggcggcga cgtctgccgt acgctggtgg ctaacggcgc gattcccctc attcctggtt
     7861 tgtggttagt acccggactc atgctaatgg gcctgctgat gctggtcgca cgcgacttct
     7921 ctttgctgca gaaattttcc cgatgaatgt tttcagccgc tatttaatcc gtcatctctt
     7981 tctcggtttt gccgccgccg cagggctatt gctgccgctt tttaccacct tcaacctgat
     8041 taacgaactg gatgatgtca gcccgggcgg ttatcgctgg actcaggcgg tgctggtggt
     8101 gctgatgacc ttaccgcgca cactggtcga actttcgccg tttatcgcct tattgggagg
     8161 gattgtcggc ctggggcaac tatcgaaaaa cagtgagctt accgccattc gcagcacggg
     8221 gttttctatc ttccgtattg cactggtggc gctggttgcc gggatattgt ggactgtttc
     8281 gttaggcgcg atagatgagt gggtggcgtc gccattgcag caacaggcgc tgcaaatcaa
     8341 atcgactgcc actgcgttgg gggaggacga tgacattacc ggcaatatgc tgtgggccag
     8401 gcgtggtaat gaatttgtga cggtgaaatc gctgaacgag cagggccagc cggtgggcgt
     8461 ggagatattt cattaccgcg acgatctctc gctcgaatcc tacatttatg cacgcagtgc
     8521 caccattgaa gacgacaaaa cgtggatcct gcatggtgtg aatcataaaa aatggctgaa
     8581 tggcaaagaa acgctggaaa cattagataa tcttgcctgg caatcggcct tcaccagtat
     8641 gaatcttgaa gagttatcaa tgccgggcaa tactttttct gtccgtcagc ttaatcatta
     8701 catccattat ttgcaggaaa ccgggcaacc cagcagcgaa taccgccttg cactgtggga
     8761 aaaactgggg caacccatcc tgaccctggc gatgattttg ctggctgtgc cgtttacctt
     8821 tagtgccccg cgttcgcctg ggatgggtag ccgtctcgct gtaggcgtca tcgtgggctt
     8881 acttacctag atcagctatc aaatcatggt caatctggga ttgctatttg cgttgagcgc
     8941 acctgttacc gcgctcggtt taccgatagt gtttgtgctg gtggcgttga gcctggtgta
     9001 ttggtatgac agtcaacatt aaacccgccg tggcgcagca ccacagcggg ctcacattac
     9061 tgcttcagag tagcaaatgc ctgaatgatg gcatcgattt gctcacgact gtgggcagcg
     9121 tttacgctac agcgcagcag cgtgatgcct gcaggcgcgg caggtggcag gataagattc
     9181 acataaacgc ccagcgaaat aagctcgcgc cagatgcgta aaccttcctc tttcgaaccg
     9241 attattactg gcactaccgg gctaatatgt gaacttaact cataacccag tttttgcaga
     9301 ccgtcataca gatggttggc gttatccatc agcttttgcc gcagctctgg ttgtgaggca
     9361 atggttctta acgacgagcg cacggtcgca atgcaggacg gagaaggaga ggcggtaaaa
     9421 atatacgggc ggctgccgta gcgcagcact tccatcgctt ccgaaccgac agcaaaaccg
     9481 ccaatcgagg ccagactttt actgaaggta ccaacgataa tatccacatc atcttcaacg
     9541 ccgacagctt cagccagtcc acgccccgtt gcacctaaca cgccaaacga atgcgcttca
     9601 tcgacaatca aataaccacc gagacgacgt ttgatatcaa caatttccgc cagcggcgca
     9661 acgtcaccga gcatgctgta gatgccctca acgatgataa tcgcctcttt cgcccgttca
     9721 ccgaggcgaa ccatgcgccg ttccagatct ttagcatcgt tatggcggaa acgaataatt
     9781 tccgcgccac cgagagaaca ggcgtcataa atactggcgt ggctgtcggc atccagtaac
     9841 accacggcgt tatggtcggc aagtgcgctg atgacgccta aattagcggt ataaccggtt
     9901 gaaaaaacga tagcggtagg gcgattaaaa aactcggcga tctctttctc taacgccaga
     9961 tgcggcgcat aactgccgtt tgccatacga gaaccggtgg tgccggtgcc ttgggccgct
    10021 aatgctgcct gaccttcggc gatggcatca tgattaaagg ttaaaccgag ataattattg
    10081 gtcccggcca gaatcacttt tttattgccg atccgacctt cggtggcgga atacacttca
    10141 tcaatacagg tgccaaacgg tgttaacccg ttgtcactaa attgcagtcg ctcgccagca
    10201 aggcgcgcaa atttatcgta tagccccatt atttattctc cagccatgga agtagtgttt
    10261 ccattaattg cgcaggggtt ttgacatcca gcagaatatt aataggaatg gaaatatcga
    10321 atctgtcttc cagcatcatt aagagatcca tcactttgat cgactccagg ccaagatcgt
    10381 tgacgagatc gctgtcgggc ttaatttcca caccgttttc gaccaaatcc tgtaaacagg
    10441 aaaggatgta atccattact atttcacgat ttaccataga aacgttaaca cacctttaaa
    10501 caagacaatc aaaatagccc gttgcgtaat gcgtgttcca ggctaatccc gggaaaccaa
    10561 ttaatatctt cagaaatacg gttattattt gccgaccagt cggcgtgggt taattcacgg
    10621 attttcgagc gggtcagcat aggttcttta cctgccaggc gactcaacgc ggtactgata
    10681 tccgcaaggc aggtgagtac cgacagagga ataccaacca ttcgaacgga accgcaacgg
    10741 gcgtcggcgg caagctgtct tacgcgttgc cagtcatagc cgccggcgac gccatcgcat
    10801 aattcatagg tttgcgtctg tacagtttcg gcgcttaacc actgccccac ggcttgcgcg
    10861 aaatcggtga cgtgaagaaa agagagctgt gtctctggcg tgccaagtcg tggcagcagg
    10921 ccgcgcagca tccagtcaaa cagcggtttt aactctttat cgccgggacc atacacggct
    10981 gtcgggcgaa aaacgcccag cgtaatttca tcagccattg cagtcagccg ttgttcggcg
    11041 acgtgtttgg aatttgcgta ccaggagagt tcgggatggc gcgccgccag cgaagagata
    11101 aacagaaaac gttggcaaaa gccgctctct tttgccgctt gcatcagtcg caggctgccg
    11161 tcaacgttac agcgggtaaa aatctcttct ttgtgcccgc gcacttgccc ggcgcaatgg
    11221 accaccgcgc tggctccggc aaccagttcg ctaagtgaat gagtatcttc cagcgaaccg
    11281 cgcacccaga tgagattatc attgacgtga gcgcgggcag tacgcgtcaa tgcgcgaaca
    11341 tgaaagccgc gggcgagcag gttatcgata atatatttac cgataaaccc ggtagcgccc
    11401 gtcaccgcga cagtttgatt catgccaggg attcctgcac atgaaggctt gcagcataag
    11461 ccttctgata acgttttttc gcttccgcac gggcaggctt gccggaggac gttcggggaa
    11521 tactgtgggg cggcaacaga tcgatagccg cggtcacgcc aaattcgctt tggatccgtg
    11581 ctgccagcgc gtggataagc tgcccgcgcg tggataagct gcccgcgacg ttcttcatcg
    11641 ctgatccgac actggatctg caaaatgatt ttttcctggg cggtaacaaa agcaatcgca
    11701 tcgccagaat gaatttccgg ttcctgttcc gcaatgtatt caatatcctg cggccagata
    11761 ttacggccac gaataataat cagatcttta atgcgtccgg tgacatacag ataaccgtcc
    11821 agcagataac cgaggtcgcc ggtgtctaac cagcccgtcg cggcaatctc gtcttgtgaa
    11881 atctggtcgc caaagtaacc gctcatcagg ctagggccgg agatgcaaat atggcctacc
    11941 acacgttccg cgacaggtat acctgcttca ttgcggattt caatgccatg ttccggcaac
    12001 gctttaccgc agttgacgaa agtcgatacg gcgcgtgttt cagcatccgg cgcgacggct
    12061 ttgccctgat attcaaggat gtcgcgatcc acttcgttaa ccacaacccc ggaagcttca
    12121 tcagagaagc tgacagccag cgcattttct gccagtccgt agcacggcat gaaagtttta
    12181 tcgtcaaagt tgacctgacg gaaacattca gcgaattgat ggagttgttc tgcggagatg
    12241 ggttctgcgc caataccagc gacgcgccag caggaaagat ccagttcagc gagatctttt
    12301 tcattcacgc ggcgctggca caattcatag ccaaacggcg gcgcaacgga aacggtgccg
    12361 cgatttttac tgatcaattt aagccattgc agaggacgca tggcaaaatc ctgagtgcgc
    12421 aaataatcta ctgaaagctg cgtggcgacg ggggtcagga gaaagccgac cagtcccata
    12481 tcatggtaga aaggcagcca ggagacgcag cggtcgccag ggcgtaattt aatgccatcg
    12541 tggcttatag cacgtagatt agccatcact tcgcgatggg tgataatgac gccacgggga
    12601 aaacgggtgc tgccggaggt gtactggagg taggcgatat cgttcggaac tggacgctgg
    12661 agcgcaacat cggcttccgg taatgcctta aaccaggcgt ggcttaaaac atgtaattcg
    12721 gggttgttgt tatgcgtcgc ggcattgacc agcggtaacc actcatcacc agtgataatg
    12781 gctgcgggct ggcaacttgc cagtaaaccc tgcagtttgg cgctccagga atcccgctga
    12841 ccgacgccca ttggaatcgc caacgggacg gcgactaagc cggcatactg gcaggaaaaa
    12901 aaagcctcta caaacccgct actcgtttcg gcaatcagcg ccacgcgatc gccttttttc
    12961 aggttcagcg ataacaaccg cttcgcacca gcttcggcac gcgcttttaa cgtctgatat
    13021 tccagttgat cttcaagttg gcaacgtctg tcataaaaat tcattccggc gctactcaga
    13081 gcggcgtagt ccaaagcatc aaccagcgtt ggaaaatcgg catagcgcat aggtagggaa
    13141 tgcgtaaaga ttttattaga catatacacc acgaaaaaat tggatatcaa aaataaaatg
    13201 acaataatgc ggtggattat ataggcataa aagataaatg agata
//
LOCUS       DAOTRK010000126        13187 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.127, whole genome shotgun sequence.
ACCESSION   DAOTRK010000126 DAOTRK010000000
VERSION     DAOTRK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.127"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(339..911)
                     /gene="hdeD"
                     /locus_tag="QIE41_003592"
     CDS             complement(339..911)
                     /gene="hdeD"
                     /locus_tag="QIE41_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDS3532435.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGVALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1166..1498
                     /gene="hdeA"
                     /locus_tag="QIE41_003593"
     CDS             1166..1498
                     /gene="hdeA"
                     /locus_tag="QIE41_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDS3532436.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1614..1940
                     /gene="hdeB"
                     /locus_tag="QIE41_003594"
     CDS             1614..1940
                     /gene="hdeB"
                     /locus_tag="QIE41_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDS3532437.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2004..2651
                     /locus_tag="QIE41_003595"
     CDS             2004..2651
                     /locus_tag="QIE41_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDS3532438.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLNHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2703..3473)
                     /locus_tag="QIE41_003596"
     CDS             complement(2703..3473)
                     /locus_tag="QIE41_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDS3532439.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPASHSPLIVLDH"
     gene            complement(3470..4426)
                     /gene="chuU"
                     /locus_tag="QIE41_003597"
     CDS             complement(3470..4426)
                     /gene="chuU"
                     /locus_tag="QIE41_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDS3532440.1"
                     /translation="MGLSLLLLALVLFGASQGALRISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIMATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLISSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4511..5134)
                     /gene="chuY"
                     /locus_tag="QIE41_003598"
     CDS             complement(4511..5134)
                     /gene="chuY"
                     /locus_tag="QIE41_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDS3532441.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVVRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGQDALIISTMGGTQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVHRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5134..5628)
                     /gene="hutX"
                     /locus_tag="QIE41_003599"
     CDS             complement(5134..5628)
                     /gene="hutX"
                     /locus_tag="QIE41_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDS3532442.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVKNLPSSTIVSG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(5641..6978)
                     /locus_tag="QIE41_003600"
     CDS             complement(5641..6978)
                     /locus_tag="QIE41_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDS3532443.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIINILREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(6998..7912)
                     /gene="shuT"
                     /locus_tag="QIE41_003601"
     CDS             complement(6998..7912)
                     /gene="shuT"
                     /locus_tag="QIE41_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HDS3532444.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATYQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            8595..10577
                     /gene="chuA"
                     /locus_tag="QIE41_003602"
                     /gene_synonym="shuA"
     CDS             8595..10577
                     /gene="chuA"
                     /locus_tag="QIE41_003602"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDS3532445.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSASDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            10626..11654
                     /gene="chuS"
                     /locus_tag="QIE41_003603"
     CDS             10626..11654
                     /gene="chuS"
                     /locus_tag="QIE41_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDS3532446.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIRDILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRSERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     TPLELKAGDAPVVQMRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAETWVTRKPASDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(11715..12245)
                     /locus_tag="QIE41_003604"
     CDS             complement(11715..12245)
                     /locus_tag="QIE41_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3532447.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12398..12964)
                     /gene="slp"
                     /locus_tag="QIE41_003605"
     CDS             complement(12398..12964)
                     /gene="slp"
                     /locus_tag="QIE41_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDS3532448.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3528 a         3089 c         3194 g         3376 t
ORIGIN      
        1 atgcaatgta atcaacgcca gttgcaaaaa atgaacccgg attacttatt taaccgccca
       61 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
      121 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggaatg
      181 aaaaaaatta gatgaaattc agtaggttga aataatcatc agcaggtaat tatttcaatg
      241 atagtgcgca attgatctac aacgctgtgt agcggagaga gtattaatcg gatcatagtc
      301 acatcaagtg actatgatcc gggtgacaac aggaataatt attgctgctt aacgaacaaa
      361 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caatacccac cagtgtggaa
      421 accaaagtta cagacaccat cggcgttgca ccaaggaata tccatgcaat gacgatatcc
      481 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
      541 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
      601 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
      661 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa tccaacaata
      721 agcgcaacgc ccgagcagat taataatgca cccactaccg tgcttaaaat atcgccagag
      781 acgaacggga aactgataca cagcaacccg acgataaaca gcaacacggc aataaactgg
      841 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
      901 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
      961 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaatta cagtatctga
     1021 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     1081 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     1141 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     1201 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagccg ataacaaaaa
     1261 accggtcaac tcctggacct gtgaagattt cttggctgtg gacgaatcct tccagccaac
     1321 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     1381 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     1441 agccaacttt aaagataaag ttaaaggcga gtgggacaaa attaagaaag atatgtaatt
     1501 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     1561 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     1621 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     1681 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
     1741 ttgatctgaa tccaaaagca atgacaccgg ttgcatggtg gatgctgcat gaagagacag
     1801 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     1861 tgatcgaata ctgcaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     1921 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     1981 gctgctcctt tttgcttttt tatatgacag cagaatttat tatacgtctt atactcgcag
     2041 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     2101 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     2161 ttgctgacgt gctgtcttta aatcacgtcg gactggaccc cagccgtatt gctgctcagg
     2221 tggtgacggg cgtcgggttt atcggcgcag gtaacattct cgttcgtaac caaaatattg
     2281 taggtctgac gacggcagcg gatatctggg tgaccgccgc cattggtatg gttattggta
     2341 gcggtatgta cgaactgggc atttatggtt cagtgatgac cttgctggtg ctggaagtct
     2401 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
     2461 acggtaatac ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
     2521 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
     2581 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
     2641 caattagcta atctttatag ttatttattc acaggagtac cagagcggta ctcctgttgg
     2701 ttttaatgat cgaggacgat caggggtgaa tggctggcag gatctttaag tacggtaata
     2761 tccgcgccgt aaagcatggt cagcgcctgc tgtgtcaata cgtcctgtgg tttaccattt
     2821 gcgataacct ttcctttttg cattaataca acacggtctg cataacgcgc ggcaagattt
     2881 agatcgtgca atacgcagca aacattgaac tgccgttcgt gaactagctg gcgtaataaa
     2941 cgaaacaagt gctgctgatg gtgaatatcc agcgccgaag ttggttcatc aagaaataac
     3001 catttcggtg agggagtagg ttcccagagt tgcaccagca ggcgcgctaa ttgcacgcgc
     3061 tgttgttcac caccggataa ttgccgatag tcacggtttg ctaacgcctg acagtcacat
     3121 aacgccatta tttgtgctgt ttcattatcc tgattaccag tgcgatgcgg atgccgcccc
     3181 atctggataa cttcttgtac gctgaatgga aacgccatgt ggctattttg acgcatcacg
     3241 gcccggtgtt tcgccagttc agtaatcgac cattcattta atggcttatt aaacaaccga
     3301 cattcgccgc tatcaggttg aagatagccg gttaactggc gaagcagcgt agattttccc
     3361 gcaccattag ggccgagaat ggcaacaatt tccccaccgg gaaaagtcag cgagacattg
     3421 tcagtcagac gccgcccctg aaggctataa accagattct gggcgctgat catgtgatcc
     3481 tccggttgcg cagaatcaac cacataaaat aaggaccgcc aagcaggctg gttaataatc
     3541 ccacgggcat ttctgctggc tgtaccagcg tgcgggcaag cgtgtctgcc atcagcaata
     3601 aacaggctcc ggcgagggcg gagcaaggga ttagccagcg gtgatttgcc ccggtagtca
     3661 tacgaatcag atgcgggatc accaggccaa taaagccgat aatgccgctt accgatacgg
     3721 cggcaccaac gagcagtgag ctaatcaaca gtaattgttg gcgtttacgc tttacgttca
     3781 cgccgaggta atgggcttct tcatcaccaa gttgcagcaa attcagcgtt ccggcgagac
     3841 acgttgttgc cataatggcg ggcagaatga aggaactggc aaccaataac gttggccatt
     3901 gcgcctgacc gagattgccc atcatccaca acgtgagttg tcgtagctgt tgctcatcgc
     3961 cgatatagct caggatgccg atcgctgcgc cgcacagggc attgatggcg ataccggcga
     4021 gtaacaattg catcatgctg ccatcgcgat ggcgctgcgt gatgagaaaa atgatggtac
     4081 agaccactaa acttccggcg atggcaaaca ccatttgctc atagagcacc agcaccactg
     4141 ggaaggagaa gggcagcaca atagcaacgc caaccattaa tgcagaaccg ctactgacgc
     4201 caagtaatcc tgggtcagcc attgggttgc gaaaaagccc ctgcataatt accccggcgg
     4261 ttgccaacgc tgcacctacc agcaccgcca gcaaaacccg tggtagacga atattgagcc
     4321 agatatcacg gtattcctca tcaaaaagag catcaaaact gatccttaac gctccctgac
     4381 tggcaccgaa cagcaccaga gctagcaata atagtgatag ccccataaag accctggcgg
     4441 aagagagtga atccttgagc acgcaaaact ccgttacgac attacggcgc ggacatgccg
     4501 cgccggggta tcacgccggt ttcaggtccg gttcaatcag actgtagacc tgctggttaa
     4561 gtgctggcgc atttgccagt tcatggatat gtgcggcgac atcggcacgg tgaacaaaac
     4621 catgacactc ctgattttgt atccgttgtg ctttgcccgt tgccgcccca tcaagcagac
     4681 cgccagggcg gagaatggcg taatcaagct ggctggtttg caaccagctt tccgccagcg
     4741 ttttttcccg taccgcctga ccaaacgcag cctttgcccg ctccgataaa aacggccagc
     4801 tatcgccaca tcccaaagag gtcaccagaa tcatgcgact gatgccggcc ttttcggcct
     4861 catcaatcac tgttctgtgt gccagataat cctgagttcc gcccattgtt gagatgataa
     4921 gcgcatcctg gccagctgcg cggcaagcag ccgcgactac gctggcatca caggcgtcgc
     4981 ctgtaaaaac ctgtacgcct tgttgcgcca gtttcgtggc ggcatcagca tgacgaacga
     5041 cagcaacgac cggacgttgt tccgccagcg ccagttcaag cgttctggca cccacacctt
     5101 ttccgcctgc gccaaagagt agccacggcg tcatcaggcg tgttccttta acgatgctgc
     5161 cagagcatgg aaggcgctga cttgttcgct caggagctga cgatggtcat ccctgccgag
     5221 gaagattttc agcattgcgc tgccttcttt gttgaaaaag agaatggatg cggtgtccat
     5281 gcccataaat ttacgttcga tcaaggcaat atgtgtgcag ttttctgctt tgatatgccc
     5341 ggacatgccg tgctttccgc gtaggttgaa atagccgtgg cggtggaaac ccgaaggcag
     5401 ttcgccgcta aattcgagga taacatcggc agtatgtact aacgtggtga cgttgcccca
     5461 ttcgcagacg gtatcccaga cagtatcgaa tttatcgcct gacactatgg ttgaggaggg
     5521 caggtttttc actacttcca gcagtgtggt gttgtattgt tcggctacca cctccagcgt
     5581 accgtccggc tccgttttca aaaattcttg taacgagaca tggctcataa atcactcctg
     5641 ttatggtttt tcacgcacaa tcgctggcgc atttagtacc tgaattagtt cattaagaga
     5701 ctgcaaaata ttgcttgccc agaaacgacc ttcattagtc agccgcaggc aagttgacgc
     5761 atcgcggctt aagccttttt ggtgccattg agccagtaac ggcgcgagtt tttcggcatg
     5821 tggcgtaagt tcatccagcg gtacacgcgc tgtttcaaca cctgactgca aggtatgacg
     5881 ccattgcgca ttacgttcgg cgttacgcat gatcatcatc agcggttttt tgcctgccgc
     5941 gacggattca tgccaggtct gaagattgcg ttcgttcatc caggagtaac cattaatcga
     6001 accgccggct ccggaaccaa aggccagaca atcggcacct tgtttaatca gcaggttata
     6061 gagattgcgt tcgcgtgtgg tacggcccca gtggctgtta ctgatgcagc gccaaccggc
     6121 atcgtccata aaatcacacc cttgcagata aagatcgcga cgttctgccg gagagggcac
     6181 ggtagtacgc ccattttcca cggctttgcc cagcggtgta ttgggcagga cattgagcgc
     6241 atagagatcg acgccgtcga gaccgatatc gcgggcaata gccagatctt ctccccaggt
     6301 ttgcgcatcc tgacccggca ggccaaacag caggtcacag accaccgcag cacggtcacg
     6361 tttgaccagg ctttccataa acgcaatggc ggttgggcca tccgaggtgc gggccatttt
     6421 cttgcggatt ttgctgttaa acgactggat accaattgag aagcggtttg ctcctgcatc
     6481 aagacaagca tcgattcgct cagcgtcaaa attcagtacc cggccttcga tagtgatttc
     6541 acaatcaggg gccagtggca gcttttctcg taatatattg ataattctgg ctaaatcgtg
     6601 tgcagaaagc gcggaaggcg tgccgccacc gaaatagacc gcgtggatgg gggcagactg
     6661 atgcaatacg ctgtccgctt ccatttctat ttcacgaatc agggcgtcgg tataatgagc
     6721 acacgcatct tcattaaaac ggttctgata aaaaccacaa aacgtgcagt gtgttgcaca
     6781 aaaaggaatg tgtagataaa ccagtcgctt acgtggtgat accgtttgat tgagcatctc
     6841 ttgccaggtc tgtgccagtt gctctttggc aaccggaatg gccccacgaa acggcatcat
     6901 ggcgcgcctg tctttaaatg gctgatcacc gtctaaagca aaatgtggcg tgagatccag
     6961 ggtgttattt gcgttcattc gtctgattta aatccattta ttgcggccaa agctgctggt
     7021 gtaaagactc aacgacatca gcaatacgcg gacccattcc cagaatcagg ttttgatcaa
     7081 cggtgataat gcgctggttt ttccatgcgg cagtgtgggt aattccggca atagaacgta
     7141 gccgattaat atcgccatcg accatttgtg aggttacgac aatcacttct ggattggccg
     7201 caatcagtga ttctgcactg tagcttttgt actgctggta ggttgcgacg ttttctgctc
     7261 cggcaagcga aagaatggcg tccgcgacgc tgcctttacc ggcaacctgt ggcgcacttc
     7321 cgcctgcgga gagaataaac atggctttaa ccggggcttt tttggccgcc acgttttgct
     7381 gtactcgctc caggcgttgg ttgatctgtg tcaccagcgc ctcgccttgt tcaggaacct
     7441 gtagcgtttt tgccagttgg cgaatgttgg cgtacatctg ctcaagggtg gcaggcacac
     7501 gcggcagggt gacgacgttg actttctgcg ctcgtaattg gtcgagcaca atttgtggcc
     7561 cggcatcctg ccaggtaatt acgctatccg ggcgaagtga caaaatgcct tcgctgctga
     7621 gttgtttcca gtagccaata tgcggcagtt tggcggtttc tggtggataa gatgtcgttt
     7681 catcgacacc aaccacgcgt tcgccagcgc ccatcgcgta gattagctcc gtcagcgatc
     7741 ctcctgcgac cacgatacgt tcggcagccg ttacgctaaa agcccagcta agagccagta
     7801 ctgccgtaaa caattttttt cgttttaccg cggtcttcgc ggcagaggcc acggctgaaa
     7861 tacataacgt cagtggggaa aaaagaaacg gcctggtgat gatccttggc ataatatgag
     7921 aactcttatc aagttgagaa tgatcatgat tatcatttga gttatatatg aaatacaacc
     7981 gtcgattcaa tacgcacggc gttacaagcg tatttatttt tgatttttat caagatgctt
     8041 cgtcagctaa cgttcttgtt accgactaaa tttcagcgcg ggaaaaagct gagaacccag
     8101 agatatcgag gcttgcagag caaagcgaag aggaaattat cactgttaac gacaaagttt
     8161 agtgatgagg tcatgggtga ataaccataa aaataaagtg gttttcaggg cgcgggatag
     8221 gtttcataac cccctgttta ttgtgttaat ggcggttaaa aaaagcttga tcataaaaaa
     8281 ttgatcgata ataattctca ttatcacgtt tgcgcttaac aaaacacgac catatggata
     8341 acaaaaccat agcatttgca agggataacg cacagcgctc ttcactcctg atggggcata
     8401 tcgttttgcc tgacaaagag ggcgtgcttt tgcgtatttg ccgagatacc aggcaatttc
     8461 atgggatata aacgcctgta aactcacccg gcgaatatta tcgctctgaa ttttaaataa
     8521 atttattttt cgccagccac cccggtatta acccaggcag ccagcatttt ttattttcct
     8581 catggagaat cgctatgtca cgtccgcaat ttacctcgtt gcgtttgagt ttgttggctt
     8641 tggctgtttc tgccaccttg ccaacgtttg cttttgctac tgaaaccatg accgttacgg
     8701 caacggggaa tgcccgtagt tccttcgaag cgcctatgat ggtcagcgtc atcgacactt
     8761 ccgctcctga aaatcaaacg gctacttcag cctccgatct gctgcgtcat gttcctggaa
     8821 ttactcttga tggtaccgga cgaaccaacg gccaggatgt aaatatgcgt ggctatgatc
     8881 atcgcggcgt gctggttctt gtcgatggtg ttcgccaggg aacggatacc ggacacctga
     8941 atggcacttt tctcgatcca gcgctgatca agcgtgttga gattgttcgc ggaccttcag
     9001 cattactgta tggcagtggt gcgctgggtg gagtgatctc ctacgatacg gtcgatgcaa
     9061 aagatttatt gcaggaagga caaagcagtg gttttcgtgt ctttggtact ggcggcacgg
     9121 gggaccatag cctgggatta ggcgcgagcg cgtttgggcg aactgaaaat ctggatggta
     9181 ttgtggcctg gtccagtcgc gatcggggtg atttacgcca gagcaatggt gaaaccgcgc
     9241 cgaatgacga gtccattaat aacatgctgg cgaaagggac ctggcaaatt gattcagccc
     9301 agtctctgag cggtttagtg cgttactaca acaacgacgc gcgtgaacca aaaaatccgc
     9361 agaccgttga agcttctgat agcagcaacc cgatggttga tcgttcaaca attcaacgcg
     9421 atgcgcagct ttcttataaa ctcgccccgc agggcaacga ctggttaaat gcagatgcaa
     9481 aaatttactg gtcggaagtc cgtattaatg cgcaaaacac ggggagttcc ggcgagtatc
     9541 gtgaacagat aacaaaagga gccaggctgg agaaccgttc cactctattt gccgacagtt
     9601 tcgcttctca cttactgaca tatggcggtg agtattatcg tcaggaacaa catccgggcg
     9661 gcgcgacgac gggcttcccg caagcaaaaa tcgattttag ctccggctgg ctacaggatg
     9721 agatcacctt acgagatctg ccgattactc tgcttggcgg aacccgctat gacagttatc
     9781 gcggtagcag cgacggctac aaagatgttg atgccgacaa atggtcatct cgcgcgggga
     9841 tgactatcaa cccgactaac tggctgatgt tatttggctc atatgcccag gcattccgcg
     9901 ccccgacgat gggcgaaatg tataacgatt ctaagcactt ctcgattggt cgcttctata
     9961 ccaactattg ggtgccaaac ccgaacttac gtccggaaac taacgaaact caggagtacg
    10021 gttttgggct gcgttttgat gacctgatgt tgtccaatga tgccctggaa tttaaagcca
    10081 gctactttga taccaaagcg aaggattaca tctccacgac cgtcgatttc gcggcggcga
    10141 caactatgtc gtataacgtc ccgaacgcca aaatctgggg ctgggatgtg atgacgaaat
    10201 ataccactga tctgtttagc cttgatgtgg cctataaccg tacccgcggc aaagacaccg
    10261 ataccggcga atatatctcc agtattaacc cggataccgt taccagcacc ctgaatattc
    10321 cgatcgctca cagtggcttc tctgttgggt gggttggtac gtttgccgat cgctcaacac
    10381 atatcagcag cagttacagc aaacaaccag gctatggcgt gaatgatttc tacgtcagtt
    10441 atcaaggaca acaggcgctc aaaggtatga ccactacttt ggtgttgggt aacgctttcg
    10501 acaaagagta ctggtcgcca caaggcatcc cacaggatgg tcgtaatgga aaaattttcg
    10561 tgagttatca atggtaatca tctgccccga tatttcgggg catttatctg gaaggaagag
    10621 agacaatgaa ccactacaca cgctggcttg agttaaaaga acaaaatccc ggaaagtacg
    10681 cgcgtgacat cgcaggttta atgaatatta gcgaagcaga actggcattt gcacgcgtca
    10741 cgcacgatgc gtggcggatg cgcggcgata tccgtgacat tctggcggcg ctcgaaagtg
    10801 ttggcgagac caaatgtatt tgtcgtaatg aatatgcagt ccatgagcaa gttggtgcgt
    10861 tcacaaacca gcatttgaac ggacatgccg gattgatcct caatccacgc gcgctggatt
    10921 tgcgtctgtt tctcaatcaa tgggccagtg tttttcacat taaagaaaat acgactcgta
    10981 gcgaacgcca gagtattcag ttctttgatc atcagggcga tgcattacta aaagtttatg
    11041 ccaccgacaa taccgatatg gcggcatgga gtgagcttct ggcacggttt atcgccgatg
    11101 agaatacgcc gcttgagtta aaagccggtg atgcgccagt cgttcaaatg cgagccgatg
    11161 ccagtgtggt tgagcaagag tggcgggcga tgaccgacgt tcatcagttt tttacgttgc
    11221 tcaagcgcca caacctgacg cgccaacagg cgttcaatct ggtggcagac gatttggcct
    11281 gcaaagtatc caacagtgcg ttggcgcaaa ttcttgaatc tgcacagcag gatggcaatg
    11341 aaatcatggt gtttgttggc aaccgtggct gcgtacagat tttcactggc gtggtagaaa
    11401 aagtggtgcc aatgaaaggt tggctgaata tttttaaccc gacgtttact cttcatctat
    11461 tagaagagag cattgctgaa acctgggtaa cccgtaaacc tgctagtgac ggttatgtga
    11521 ccagtctgga attgtttgcc catgatggta cgcagatagc gcaactttat ggtcaacgta
    11581 cagaaggcga acaggagcaa gcgcaatggc gtaagcaaat tgcttcgctg ataccggaag
    11641 gcgttactgc ataacgtata attaacggga ggtctggcta tttattgctg ggcctcttta
    11701 tattaatcaa tcgatcacac cagataatca atatgctgat ggcgtaccaa ctcattgatc
    11761 cgcttaagat tgaggatcat catgatattg tgtttatggc agtagaacgt ttttagcgat
    11821 attttcagtt tctccagaat atcttccgtt gtcatttgat tcgcaatgca gctcagaacc
    11881 tgatgctgct tgcgcgaaag ggacacatcc gcttcaggca cagagcagta actgccattg
    11941 atgagtgcga caaagtcgat gatggtgata gttcgcggaa caaaggtcac cggtactttc
    12001 cccaggcagc gtttaatatt aaagggggag aaaataatca catggacagg ttttaatcgt
    12061 tcaatttgag tgagcaaatt agaatgaaat acattattca ttaatgtatc aataatgacg
    12121 aaggcatgct gatgcaacat tacgtcgatc tcttcttcgt tctgtatatg aacgacatta
    12181 cctttactca gaatgttttt catcgcagtg aagaacatcg tatccctggt aattataaga
    12241 aacataaccg actcctggtg cggacattaa ctttataatc agtttgccaa aggtaattaa
    12301 caggacaggg cttataataa tagttagctg gctgtgtatt aatttgatgt aatgcatgat
    12361 gcagatgctg agaaagcatc tgcatctctc ggtggtgtta tttgaccagc tcaggtgtta
    12421 cctgactcac cgcattggtg tagtaaggcg cgccccagcc cggttccggc cagaatgcac
    12481 catagccgta atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct
    12541 ggatgccctg catattcact tcgaggaagt tatacgggac tttattgata aagccaggtt
    12601 gttcaccctg aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa
    12661 ggaagccgct ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg
    12721 catagctatc caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa
    12781 cgttgataac cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat
    12841 gaacagcaac aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa
    12901 ttgaactaca tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt
    12961 tcatgttact atccttatca acaaatatta aaaccataat agatatttct ttatagtttt
    13021 catctgattc tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc
    13081 ttctgtttag tttaattctg ttaattttgg ggcgtgacat gagtatccat cgccccataa
    13141 aatagccttt ttttaaaggt acataaaatc tatttttatt caatgct
//
LOCUS       DAOTRK010000127        13088 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.128, whole genome shotgun sequence.
ACCESSION   DAOTRK010000127 DAOTRK010000000
VERSION     DAOTRK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.128"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            151..393
                     /locus_tag="QIE41_003606"
     CDS             151..393
                     /locus_tag="QIE41_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532449.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            507..1145
                     /locus_tag="QIE41_003607"
     CDS             507..1145
                     /locus_tag="QIE41_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDS3532450.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     DVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEDVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1142..1879
                     /locus_tag="QIE41_003608"
     CDS             1142..1879
                     /locus_tag="QIE41_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDS3532451.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSSADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSKTPDALNADILQTLTQRIPQ"
     gene            1879..3975
                     /locus_tag="QIE41_003609"
     CDS             1879..3975
                     /locus_tag="QIE41_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDS3532452.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4022..4300)
                     /locus_tag="QIE41_003610"
     CDS             complement(4022..4300)
                     /locus_tag="QIE41_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532453.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKTDLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4301..4444
                     /locus_tag="QIE41_003611"
     CDS             4301..4444
                     /locus_tag="QIE41_003611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532454.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFAVPAMVRFVLPDAPPGAAYP
                     AYG"
     gene            4572..4982
                     /gene="psiE"
                     /locus_tag="QIE41_003612"
     CDS             4572..4982
                     /gene="psiE"
                     /locus_tag="QIE41_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDS3532455.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5076..5966)
                     /gene="malG"
                     /locus_tag="QIE41_003613"
     CDS             complement(5076..5966)
                     /gene="malG"
                     /locus_tag="QIE41_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDS3532456.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5981..7525)
                     /gene="malF"
                     /locus_tag="QIE41_003614"
     CDS             complement(5981..7525)
                     /gene="malF"
                     /locus_tag="QIE41_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDS3532457.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSTLFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7679..8869)
                     /gene="malE"
                     /locus_tag="QIE41_003615"
     CDS             complement(7679..8869)
                     /gene="malE"
                     /locus_tag="QIE41_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDS3532458.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGHPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9234..10349
                     /gene="malK"
                     /locus_tag="QIE41_003616"
     CDS             9234..10349
                     /gene="malK"
                     /locus_tag="QIE41_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDS3532459.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10421..11761
                     /gene="lamB"
                     /locus_tag="QIE41_003617"
     CDS             10421..11761
                     /gene="lamB"
                     /locus_tag="QIE41_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDS3532460.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11913..12833
                     /gene="malM"
                     /locus_tag="QIE41_003618"
     CDS             11913..12833
                     /gene="malM"
                     /locus_tag="QIE41_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDS3532461.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3141 a         3422 c         3477 g         3048 t
ORIGIN      
        1 tgaaacttat atttgatatt cattccaatt atcctaaaac cccatcgcta attccccaca
       61 ccgtaattcg catgctttag ttgtgtatac tcgatcccgc ccgaaatgtt tttgggtaaa
      121 tctccattca ttcaatgaag ggaaattgtt atgaaaaaag ttctgtatgg catttttgcc
      181 atatctgcgc ttgcggcgac ttctgcgtgg gctgcacctg tacaggtggg cgaagcggca
      241 gggtcggcag caacgtcggt ttcggcgggg agttcctccg cgaccagcgt cagcaccgta
      301 agctcggcgg tgggtgtcgc gctcgcggca accggtggcg gtgatggttc taataccggg
      361 accacaacaa ccacgaccac cagtacccag taataaagta tgtatcccca aaataattcg
      421 agtcattgca tctgtggctt gaagtatgaa gggatttacc ataaccacac tccggtgtgg
      481 ttattctgcc cctctggaga agagtcgtga agcgacctgc actcattctt atctgcctgc
      541 tattacaggc ctgttcagcc acgactaaag agctgggcaa ttcgctgtgg gacagtctgt
      601 tcggcacgcc aggcgtacag ctgacggacg atgatattca aaatatgccc tacgccagcc
      661 agtacatgca gcttaatggc gggccgcaat tgtttgtggt gctggccttt gctgaagacg
      721 gacaacaaaa atgggtcact caggatcagg ccactctcgt cacacaacat ggtcgtctgg
      781 tgaagacttt gcttggcggc gacaacctga ttgacgtgaa taaccttgcc gccgacccgc
      841 tgattaaacc cgcacaaatt gttgatggtg caacatggac gcgcacgatg ggctggaccg
      901 agtaccagca ggtacgctac gccaccgcac gctcagtctt caaatgggat ggcaccgata
      961 ccgtcaaagt cggcagcgat gaaactccgg ttcgcgtgct ggacgaagac gtctccaccg
     1021 accaggcgcg ctggcataac cgctattgga tcgacagcga agggcaaatt cgccagtcgg
     1081 aacagtatct cggcgcggat tatttcccgg tgaaaaccac tctcatcaag gcggcaaaac
     1141 aatgattaaa caaactattg tcgcattgat tttgagtgtg ggggcgtcat cggtcttcgc
     1201 ggcgggaacc gtcaaggtat tcagcaatgg cagcagtgag gccaaaacgc tgacgggggc
     1261 agagcattta atcgatctgg taggccaacc gcggctggca aacagttggt ggcccggtgc
     1321 ggtgattagc gaagagctgg caacggcggc agcattgcgt cagcagcagg cgttgctgac
     1381 acggctggca gaactagccg cagattccag cgctgacgat gccgccgcca ttaacgcctt
     1441 acgccagcaa attcaggcgt tgaaggtgac gggcaggcaa aaaatcaatc ttgatcccga
     1501 tatcgtccgc gttgctgaac gcggtaaccc gccgttgcag ggcaactaca cgctgtgggt
     1561 cggaccaccg ccgtccacgg tcacgttatt cgggcttatc agccgtcctg gcaagcagcc
     1621 attcactcca ggtcgcgacg tcgcaagcta tctctccggc cagaacctgc ttagcagtgc
     1681 ggatcgcagc tacgcgtggg tggtttaccc ggacggacgc acgcaaaaag cgccggtggc
     1741 ttactggaac aagcgtcacg tagagccgat gcccggcagc attatttatg ttggcctcgc
     1801 ggactccgtc tggagtaaga cgcctgatgc cctgaacgcc gacattcttc agactctgac
     1861 gcagcggata ccccaataat gaaaaaaaga catctgctta gcttactggc gctgggcatt
     1921 agcacagctt gctacggcga aacatatcct gcgcccattg gtccgtcgca gtcggatttc
     1981 ggtggcgtag gattattaca aacgcccacc gcacgtatgg cacgggaagg ggagctgagc
     2041 ctgaactatc gcgataacga tcagtaccgt tattactcag cttcagtgca actcttcccg
     2101 tggctggaaa caacgctgcg ctacaccgac gtgcgcaccc ggcagtacag cagcgtcgaa
     2161 gcgttctctg gcgatcaaac gtataaagat aaagccttcg atctcaaact gcgtttgtgg
     2221 gaagagagtt actggctgcc gcaagtggcg gttggtgcgc gggatatcgg cggtacgggg
     2281 ctgtttgatg cggaatatct tgttgccagc aaagcctggg ggccgttcga ttttacgctc
     2341 ggcctgggat ggggatatct gggcaccagc ggtaatgtga aaaatccgct ctgttcagcc
     2401 agtgataaat attgctatcg cgataacagc tacaaacagg cgggatctat cgacggtagc
     2461 cagatgttcc acggtcctgc ctcactgttt ggcggcgtgg aataccagac gccctggcaa
     2521 ccgctgcgcc tgaaactgga gtatgaaggc aataattatc agcaggattt tgccgggaag
     2581 ctggagcaaa aaagtaagtt taacgtcggg gcgatttatc gcgttaccga ttgggccgac
     2641 gttaacctta gctatgaacg tggcaacacc tttatgtttg gcgtcacgct gcgcaccaac
     2701 tttaacgatc tgcgcccgtc ctacaacgat aacgcccgcc cgcaatatca accgcagccg
     2761 caggatgcca ttttgcagca ttcggtggtg gcaaatcagt taacgctgtt gaaatacaac
     2821 gccggactgg ccgatccgca gatccaggcg aaaggcgata cgctgtatgt caccggcgag
     2881 caggtgaaat atcgtgattc gcgcgaaggg atcatccgtg ctaatcggat cgtgatgaac
     2941 gatctgccgg atgggatcaa aacgatccgc attacggaga atcgccttaa catgccgcag
     3001 gtgacgacgg aaaccgatgt cgccagcctg aaaaatcatc ttgccggaga gccgttgggc
     3061 cacgaaacga cgctggcgca aaaacgcgtc gagccagtgg ttccgaagtc caccgagcag
     3121 ggctggtata tcgacaaatc acgctttgat ttccatatcg atccggtgct gaaccagtcg
     3181 gtcggtggcc cggaaaactt ttacatgtat cagctgggcg tgatgggaac ggcagatttg
     3241 tggctgacgg accatctgct gaccactgga agcctgtttg caaatcttgc caacaactac
     3301 gacaagttta actacactaa tccaccgcag gactcgcact taccgcgcgt tcgtacccat
     3361 gtgcgcgagt atgtgcagaa cgatgtctat gtgaataacc tgcaagccaa ctacttccag
     3421 catctgggca acggcttcta cggtcaggtc tacggtggtt atctcgaaac catgtttggc
     3481 ggtgcggggg cagaagtgtt gtatcgcccg ctggacagca actgggcgtt tggtctggat
     3541 gccaactacg ttaaacagcg cgactggcgt agcgcaaaag atatgatgaa attcaccgac
     3601 tacagcgtga aaaccggaca tctgaccgca tactggacgc catctttcgc tcaggatgtg
     3661 ttagtgaaag cgagcgtcgg gcagtatctg gcaggggata aaggcggcac gctggagatc
     3721 gccaaacgct ttgatagcgg cgtggtggtg ggtggctatg ccacgatcac caatgtttcg
     3781 aaagaggagt acggcgaagg ggacttcacc aaaggcgtgt atgtctctgt accgctggat
     3841 ctcttctcgt ctggcccgac acgcagccgt gcggcaattg gctggacgcc gctgacgcgt
     3901 gacggtggtc agcaacttgg gcgtaagttc cagttgtatg acatgaccag cgaccgtagc
     3961 gtcaatttcc gctaagtcat gggaaaggtg ccagttttcg cactcactgg cacctttatt
     4021 cttaatggcc tgtcgagcca tcaggaacgc agttcacccc cggtttgcga tctggtgtgt
     4081 aaaggcgaaa acattctgtc ttcctgtccg tcgatattgg ttgcaggtct ggttgcctga
     4141 gcgactgggc catcattggg ctggagacat tgctttgcgg taggtcggtt ttatcagcag
     4201 taccgagcgg accagcataa gcaggaagaa cagagactga taacatcaaa gcagcaaaat
     4261 aaggcttcat ttttaccacc tttatcaggt tacgtttcat ttgttccaga ggaacattgt
     4321 cgatttttcg cgcattgctg gtggctggga atcacctgaa tgggtgattt ttgcagtacc
     4381 ggctatggtg cggtttgtct tgccggatgc gccgccaggc gcggcttatc cagcctacgg
     4441 gtaggccgca tccggcgttg atgggagatg ctctctaaat ccaggttgaa caaaacatac
     4501 acaaaaaata tagatcaccg tcacattttt gcgttataca ggaagctcgc cattgtgaag
     4561 gaggtactgc tatgacgtca ctctctcgtc cgcgcgtgga gtttatctcc accattttgc
     4621 agaccgtact caatcttggc ctgctgtgcc tcggcctgat tttggttgtc ttcctcggca
     4681 aagaaacggt gcatctggct gatgtgctgt tcgcgccaga acaaaccagc aaatatgagc
     4741 tggtagaagg gctggtggtt tactttctct atttcgaatt tatcgcgctg attgtgaagt
     4801 actttcagtc cggttttcac ttcccgctac gttactttgt ctatattggg atcaccgcca
     4861 ttgtgcgctt gatcatcgtc gatcataaat cgccactgga tgtgttgatc tactcggcag
     4921 cgatcctgtt gctggtgatc accctgtggt tatgtaattc gaaacggttg aagcgggagt
     4981 aaaaaaaggc gctccgtgga gcgccgaata acagtcacaa gttgggataa cgtaagttga
     5041 gggtgcagcg gcataacatt ggcagaacaa catctttaac ctttcacacc acctgccgtc
     5101 aggccgttga ccagccagcg ttgagccagc aagaagacga tggtgatcgg taatgcagac
     5161 atcactgcag cggcggcaaa gtcaccccac aggtagtttt gcgggttgag gtattgctgc
     5221 attcccacgg ccagggtgta gctgtttacg tcacgcagta acagcgacgc gaccggaact
     5281 tcagtaatgg cagcgataaa cgacaggatg aacaccaccg ccagaatcgg tactgacagc
     5341 ggtaacaaga caaggcggaa ggcttgccac ggtgtcgcac catccagcgc cgccgcttct
     5401 tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg tccagacatg cagcgcaatc
     5461 ccacccagat acgcgaaaat tacgccgccg tgagtattca ggccaatgaa tggaatgtac
     5521 tcacccagac gatcaaacaa cgcatacaac gcgaccagtg aaagtactgc cgggaacatc
     5581 tggaaaatca gcattccttt cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa
     5641 gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg cggaaatccc ggcgaccttt
     5701 accgagttcc acagccacag cagtaccggg aatggcggtg gcgtaatgcg accatcagcc
     5761 tgttccacac taaaacctaa cgccagtttc cagtgatccc aggagatttg ctccgggatc
     5821 agactaccgg tcgcgaagtt cccctgacgc agtgagatag cgacgaccat cagcagcggg
     5881 aacataatcg ccgcgataaa aagtagcagt agcaggtgag taataaacaa acgtgctttt
     5941 tgcgatttcg gttggaccat tgccattttt gttatctccc ttaatcaaac ttcattcgcg
     6001 tggctttcag gttcactatc gccagcgcgc ccaccagcag gaagatcagc gtggcaattg
     6061 ctgctgccag accgaagtcc tgacccccgc cgccttcaaa agcgatgcgg taggtgtagt
     6121 taacaagcag gtcggtataa ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt
     6181 tggttaacag ttgaatcagc acgaagttgt taaagttaaa ggcaaagctg gcgatcatca
     6241 gcggcgtcag cggtttaatc agcagcggca gcgtaatctt aaagaagttc tggaatggac
     6301 ctgcgccatc cattgctgag gcttcataca aatcgtccgg aatcgctttc agcaagccca
     6361 tgcagaggat catcatgtac ggataaccca gccaggtgtt gacgataatc agcatcgtgc
     6421 gggcggtggt cggatcgctg aaccaggcgg gcttcacgcc aaacagcgtg ctcaacatca
     6481 tgttgatctc accgaagctc tggttaaaca accctttgaa aatcaagatt gaaatgaacg
     6541 atggcaccgc atagggcaga atcagcagga cgcgatagac cgcttttccg cgcaacgctt
     6601 cccactgcac cagacacgcc agaaccatgc cgaccgccac cgttaaaaag acggtgatca
     6661 gcgagaacac cacggtccag acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg
     6721 tgaagacgcg ggtaaagttt ttccagccag tggtcacggt gtaaccgggg cttagctttt
     6781 catcacccca gttgccgtcg gcggtaatgg actggtaaaa gccaatttgg ttattcggac
     6841 gatatttcac gccgctctga ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg
     6901 gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac tttgttgcca tctggcagaa
     6961 tggcggtaat gtcactcagc gcctgacgat tctgggtaat cacgcgcaga ttcgcgcgtt
     7021 cgccttcggg ctgggcggtc gtttctttca gttgcagttt ttgctcgccg ccaaatttaa
     7081 acgcgtcgga gaggtaattt ttgccggttt cgccgtcgct gagcgccagt tgccactcat
     7141 cgcccgccgg gtaaagacca aaattatagg ttttgcctgc ttgccaggag cgatctaaca
     7201 acacttcctg cgcacgttca aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa
     7261 tggcgatggt gcagaccaaa gggaagagga cgaataatcc cattccagcc attcccgggt
     7321 aaacatagcg ccaggcgtag gctttacgat tggcgaaaat atacagcccc gctgaactca
     7381 atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc gtacattaaa acaacaaggt
     7441 aacccaccag caggccgagc agacctaaca ctgaccattt cagcgcgtcg ctttgccacc
     7501 aatgtttctt tttaatgaca tccatggggt tcttcctcat tccaggacgg ataaggcttt
     7561 cacgccttat ccgacaacaa ctgcctgatg cgacgctgac gcgccttacc aggcctacat
     7621 acgtttcaat ttcgtaggcc ggataaggcg ttcacgccgc atccggcatt tcacagcatt
     7681 acttggtgat acgagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg
     7741 cggcgttgat caccgcagta cgcacggcat accagaaagc ggacatctgc gggatgttcg
     7801 gcatgatttc acctttctgg gcgttttcca tggtggcggc aatacgtgga tctttcgcca
     7861 actcttcctc gtaagacttc agcgctacgg cacccagcgg tttgtcttta ttaaccgctt
     7921 ccagaccttc gtcagtcagc agatagtttt cgaggaactc tttcgccagc tctttgttcg
     7981 gactggcggc gttaatacct gcgctcagca cgccaacgaa cggtttggat ggatgaccct
     8041 tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg
     8101 cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg
     8161 agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac
     8221 ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat
     8281 acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca
     8341 ggttgaacat cagcgcgctc ttgcctttcg ctttcagttc tttatccagc gccgggatct
     8401 cttcccaggt ttttggcggg ttcggcagca gatctttgtt ataaatcagc gataacgctt
     8461 caacagcgat cgggtaagca atcagcttgc cgttgtaacg tacggcatcc caggtaaacg
     8521 gatacagctt gtcctggaac gctttgtccg gggtgatttc agccaacagg ccagattgag
     8581 cgtagccacc aaagcggtcg tgcgcccaga agataatgtc agggccatcg ccagttgccg
     8641 caacctgagg gaatttctct tccagtttat ccggatgctc aacggtgact ttaattccgg
     8701 tatctttctc gaatttctta ccgacttcag cgagaccgtt atagccttta tcgccgttaa
     8761 tccagattac cagtttacct tcttcgattt tggcgagagc cgaggcggaa aacatcatcg
     8821 tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt tattttcata atctatggtc
     8881 cttgttggtg aagtgctcgt gaaaacacct aaacggactc tagtttcttt atacagcaac
     8941 ctctttccat cctccttgcc cctacgcccc accgttgctt tgtgtgatct ctgttacaga
     9001 attggcggta atgtggagat gcgcacataa aatcgccacg atttttgcaa gcaacatcac
     9061 gaaattcctt acatgacctc ggtttagttc acagaagccg cgttctcatc ctcccgcctc
     9121 ctcccccata aaaaagccag ggggtggagg atttaagcca tctcctgatg acgcatagtc
     9181 agcccatcat gaatgttgct gtcgatgaca ggttgttaca aagggagaag ggcatggcga
     9241 gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt cgtggtatcg aaagatatca
     9301 atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat
     9361 cgactttact gcgcatgatt gccgggcttg agacgatcac cagcggcgac ctgttcatcg
     9421 gtgagaaacg gatgaatgac actccgccag cagaacgcgg cgttggtatg gtgtttcagt
     9481 cttacgcgct ctatccccac ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg
     9541 caggcgcaaa aaaagaggtg attaaccaac gcgtcaacca ggtggcggaa gtgctgcaac
     9601 tggcgcattt gctggatcgc aaaccgaaag cgctctccgg tggtcagcgt cagcgtgtgg
     9661 cgattggccg tacgctggtg gccgagccaa gcgtattttt gctcgatgaa ccgctctcca
     9721 acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc
     9781 tgggccgcac aatgatttac gtcacccacg atcaggtcga agcgatgacg ctggccgaca
     9841 aaatcgtggt gctggacgcc ggtcgcgtgg cgcaggttgg gaaaccgctg gagctgtacc
     9901 actatccggc agaccgtttt gtcgccggat ttatcggttc gccaaagatg aacttcctgc
     9961 cggtaaaagt gaccgccact gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc
    10021 gtcagcaagt ctggctgccg gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc
    10081 tgggtattcg cccggaacat ctactgccaa gtgatatcgc tgacgtcatc cttgagggtg
    10141 aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat ccatatccag atcccttcca
    10201 ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat
    10261 tcgctatcgg cctgccgcca gagcgttgcc atctgttccg tgaggatggc actgcatgtc
    10321 gtcgactgca taaggagccg ggcgtttaag caccccacaa aacacacaaa gcctgtcaca
    10381 ggtgatgtga aaaaagaaaa gcaatgactc aggagataga atgatgatta ctctgcgcaa
    10441 acttcctctg gcggttgccg tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga
    10501 tttccacggc tatgcacgtt ccggtattgg ctggacaggt agcggcggtg aacaacagtg
    10561 tttccagact accggtgctc aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc
    10621 tgaattaaaa ttgggtcagg aagtgtggaa agaaggcgat aagagcttct atttcgacac
    10681 taacgtggcc tattccgtcg cacaacagaa tgactgggaa gctaccgatc cggccttccg
    10741 tgaagcaaac gtgcagggta aaaacctgat cgaatggctg ccaggctcca ccatctgggc
    10801 aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc gacttctact actgggatat
    10861 ttctggtcct ggtgccggtc tggaaaacat cgatgttggc ttcggtaaac tctctctggc
    10921 agcaacccgc tcctctgaag ctggtggttc ttcctctttc gccagcaaca atatttatga
    10981 ctataccaac gaaaccgcga acgacgtttt cgacgtgcgt ttagcgcaga tggaaatcaa
    11041 cccgggcggc acattagaac tgggtgtcga ctacggtcgt gccaacctgc gtgataacta
    11101 tcgtctggtt gatggcgcat cgaaagacgg ttggttattc actgctgaac atactcagag
    11161 tgtcctgaag ggctttaaca agtttgttgt tcagtacgct actgactcga tgacctcgca
    11221 gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa
    11281 tatcaacaac aacggtcaca tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga
    11341 taactgggac atgatgtacg tgggtatgta ccaggatatc aactgggata acgacaacgg
    11401 caccaagtgg tggaccgtcg gtattcgccc gatgtacaag tggacgccaa tcatgagcac
    11461 cgtgatggaa atcggctacg acaacgtcga atcccagcgc accggcgaca agaacaatca
    11521 gtacaaaatt acccttgcac aacaatggca ggctggcgac agcatctggt cacgcccggc
    11581 tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa tggggttatg actacaccgg
    11641 taacgccaat accaacacta acttcggcaa agccgttcct gctgatttca acggcggcag
    11701 cttcggccgt ggtgacagcg acgagtggac cttcggtgcc cagatggaaa tctggtggta
    11761 atagcaaaaa ctgggccgga taaggtgctt gcgccgcatc cggcataaaa acaggttgtc
    11821 attatctgaa aggggcgcaa gcccctctga ttatcgggtt tagcgcgcta ttgcctggct
    11881 accgctgagc tccagatttt gaggtgaaaa caatgaaaat gaataaaagt ctcatcgccc
    11941 tctgtttatc agcagggttg ctggcaagcg cgcctggaat cagccttgcc gatgttaact
    12001 acgtaccgca aaacaccagc gacgcgccag ccattccatc tgccgcgctg caacaactca
    12061 cctggacacc ggtcgatcaa tctaaaaccc agaccaccca actggcgact ggcggccaac
    12121 aactgaacgt tcccggcatc agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg
    12181 gcgaactgac cctaacgctg accagcgaag tgaacaaaca aaccagcgta tttgcgccga
    12241 acgtgctgat tcttgatcag aatatgaccc catcagcctt cttccccagc agttatttca
    12301 cctaccagga accaggcgtg atgagtgcag atcgtctgga aggcgttatg cgcctgacac
    12361 cggcgttggg gcagcaaaaa ctttatgttc tggtctttac cacggaaaaa gacctccagc
    12421 agacgaccca actgctcgac ccggctaaag cctatgccaa aggcgtcggt aactcgatcc
    12481 cggatattcc tgacccggtt gctcgtcata ccaccgatgg cttactgaaa ctgaaagtca
    12541 aaacgaactc cagttccagc gtgttggtcg ggccactatt tggttcttcc gctccagctc
    12601 cggttacggt aggcaacacg gcggcaccag ctgtggctgc acccgctccg gcaccggtga
    12661 agaaaagcga gccgatgctc aacgacacgg aaagttattt taataccgcg atcaaaaacg
    12721 ctgtcgcgaa aggtgatgtt gataaggcgt taaaactgct tgatgaagct gaacgcctgg
    12781 ggtcaacatc tgcccgttcc acctttatca gcagtgtaaa aggcaagggg taattacgcc
    12841 ccacagtgct gattttgcaa caactggtgc gtctcctggc gcaccttttt ttatgcttca
    12901 ttccagggat atgagcgatt ttttatagta cttcacttct tcttcactaa gaatatccat
    12961 tatctcaatg cattatcaga gattcttttc ctttcgccgg tagtgtctgg acgctcaggc
    13021 tactttttcc agttatttca tttctgtaat gcagaggttt tatgttaggt catatctcaa
    13081 agtttgac
//
LOCUS       DAOTRK010000128        12792 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.129, whole genome shotgun sequence.
ACCESSION   DAOTRK010000128 DAOTRK010000000
VERSION     DAOTRK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.129"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            65..799
                     /gene="nanR"
                     /locus_tag="QIE41_003619"
     CDS             65..799
                     /gene="nanR"
                     /locus_tag="QIE41_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDS3532462.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPNNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(835..1770)
                     /locus_tag="QIE41_003620"
     CDS             complement(835..1770)
                     /locus_tag="QIE41_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS3532463.1"
                     /translation="MGFIMNKDRSLNEMMLSLFNKSTIVDLSSETISESKSLSGRIYQ
                     DPEHNGEFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYQAVMLDKEGTALAPE
                     FLSQGYTLFVLSYRLPSPAEGENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFS
                     AGGHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLG
                     DNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHL
                     YEKGGHGFSIQWATDLPVGGWIKLFMDWMYSHGFK"
     gene            complement(1820..2929)
                     /locus_tag="QIE41_003621"
     CDS             complement(1820..2929)
                     /locus_tag="QIE41_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDS3532464.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYFSKPADDYFLNNHIISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVSLP
                     WQGGMLILGGEKKDGKAVSDVIYLKKNDKQIKIVK"
     gene            complement(2942..3652)
                     /locus_tag="QIE41_003622"
     CDS             complement(2942..3652)
                     /locus_tag="QIE41_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HDS3532465.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            complement(3698..4528)
                     /locus_tag="QIE41_003623"
     CDS             complement(3698..4528)
                     /locus_tag="QIE41_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS3532466.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTGDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            complement(4532..6004)
                     /locus_tag="QIE41_003624"
     CDS             complement(4532..6004)
                     /locus_tag="QIE41_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532467.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWKKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAEE"
     gene            complement(6053..6928)
                     /locus_tag="QIE41_003625"
     CDS             complement(6053..6928)
                     /locus_tag="QIE41_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDS3532468.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYVQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            complement(6962..7879)
                     /locus_tag="QIE41_003626"
     CDS             complement(6962..7879)
                     /locus_tag="QIE41_003626"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDS3532469.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLFEEHDRNKKMKII"
     gene            7997..8395
                     /locus_tag="QIE41_003627"
                     /pseudo
     CDS             7997..8395
                     /locus_tag="QIE41_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547016.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            8541..8966
                     /locus_tag="QIE41_003628"
     CDS             8541..8966
                     /locus_tag="QIE41_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDS3532470.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLVASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            8963..9313
                     /gene="tnpB"
                     /locus_tag="QIE41_003629"
     CDS             8963..9313
                     /gene="tnpB"
                     /locus_tag="QIE41_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDS3532471.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLL
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            9344..10441
                     /locus_tag="QIE41_003630"
                     /pseudo
     CDS             9344..10441
                     /locus_tag="QIE41_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            10441..11979
                     /locus_tag="QIE41_003631"
     CDS             10441..11979
                     /locus_tag="QIE41_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDS3532472.1"
                     /translation="MNDISSDDIFLLKQRLAEQEVLIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDIAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRQLSPLEEALHGYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTCVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            complement(12018..12203)
                     /locus_tag="QIE41_003632"
                     /pseudo
     CDS             complement(12018..12203)
                     /locus_tag="QIE41_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12200..12583)
                     /locus_tag="QIE41_003633"
     CDS             complement(12200..12583)
                     /locus_tag="QIE41_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532473.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSDEGVAE"
BASE COUNT         3686 a         3163 c         2623 g         3320 t
ORIGIN      
        1 tttcattatt aaaagaaagt gttaaattaa aaactcattt gctctaatcc atataaatca
       61 atatatgtcc gacaacaaaa aaaccgtgtt acccgggcta acaacgacca gaaagcccat
      121 tgttacaaaa aaactttcag aaatcgttgc cgaagagctt gaaagaatga tccgccagga
      181 ggagataagg gaaggtgaat acctgccttc tgaacgtgaa cttatggcat tctttggcgt
      241 tggaagacca tctgtcagag aggctttatc atctctcaga cataaagggc taatccggta
      301 taacaaggga gaaaaagcca gtgtctgccg tccatcacca gaaaccatca ttaatgggtt
      361 atcaggcatt gttaaagatt ttttatctac agctgatggc atcaaggatt ttgaaagact
      421 acgcatcttt tttgaaacca accttgtaag atatgcagca aaaaatgcaa cgcctgagga
      481 tattcacagg cttgagaagg tgctcagtat ccatcataac tcactgagaa cccccgaagt
      541 gtatgctcaa tatgatattg aatttcatag tatacttgct gatataccta acaataaagt
      601 attaaaaaca atacactcag cattttctga atggctgata atggccaaac ctctggaaaa
      661 gaataaaatg acatatgaaa ataacaaaaa aacccatcag cagcatattg aaatacttac
      721 ggccataaaa gagcatgatt gtgataaagc tgaacagcta atgtataatc acctttataa
      781 acgaattgaa gcatcatagt tactcgacgc ttcaccaggt agtcaccata aatattattt
      841 aaatccatga gaatacatcc agtccataaa cagttttatc catcctccaa caggaagatc
      901 tgtggcccac tgaatactaa aaccatgccc tcctttctca tagagatgca tttcagcaga
      961 aacgcctttc tctattaggc cacggtacat gagcagactg ttttcaattg acacaagatc
     1021 atcatcacaa caatggagta aaaacacagg aggaaaacat gatgtgacat taagatcagc
     1081 actatattgt attatatttt ctgaggtact attgtccccc agtaattgct tcctggagcc
     1141 tggatgtgtc acatcatctt tcattgatat tacaggataa attaacccga caaaatcggg
     1201 aatagcgaaa atctcttgtt ctttactgtc agaagaacgt ttgaaatcac aaatctgatt
     1261 cacaaacgtt gaggcgatat gccccccagc agaaaatcct aatacaccaa tacgatgacg
     1321 ctgtagatgc catttatcag cattcttacg aatataaagc attgcagata ttgcatcaat
     1381 aaaagttttg tttatatttt ctccctcagc tggcgatggt aatctgtaac taagaacaaa
     1441 taatgtatag ccctgagata aaaactctgg agcaagagct gtaccttcct tatctaacat
     1501 aacagcctgg tagccccccc cgggaataat caatattcct gttccatttg gtttatctgg
     1561 taaaaaaata attaactgag gagcataaat atctgtcaat acacgatcaa gttcaaattc
     1621 cccgttatgt tcaggatcct gataaatacg gcctgaaaga gatttactct cagatattgt
     1681 ctcacttgac aagtcaacaa tagttgattt attaaacaag cttaacatca tttcatttaa
     1741 agatctatct ttattcatta taaaccccat taaaataact caggtgactg aacaactaat
     1801 acagtcacct gcattacagt tatttaacaa tctttatttg tttgtcattt tttttcagat
     1861 aaataacatc tgaaacagct ttaccatctt ttttctcccc ccccagaatg agcatccccc
     1921 cctgccaggg caggcttaca ccataagcaa gtccttccgg caaagaacca acttttttcc
     1981 agtcattttt tattagctga tatatctctt ttgaataggc tttatccagt cctttatgag
     2041 accagtattc accattgcta tacttttctg ccgcccccgg gaaatatgca ccaccggcca
     2101 gaaagatatt tccatcaata acagaggcat atgctcctga caccccctcg ggtgaagcta
     2161 ctggtggaag ttcagaccat ttaattcgat catgcgaaag ctcccccgtc caggatctaa
     2221 cagaacgaag ccccggtttt ctctcccctc ccagtatata aatttgctgt tctgcaaaaa
     2281 tcaccgatga accagcagtt cccggaaatg gagtcgttcc caggttcttc cactggtttt
     2341 ttgacgggtc ataagaaatt atatgattat ttaaaaaata atcatcagca ggtttgctaa
     2401 aataatccct gttgactttc tctgtcagcg cggaattccc ttttgataac tcgacatctg
     2461 aaaagtatcc attaaaaata ttctcattta caccaccagt agataccgca tgaccattat
     2521 ggataaaaga aacatgaccg gcaagagata cagggggacg ggtcatcaat ttttcccatt
     2581 tatctttagc aatatcatag gaatagacat ctttttgtaa ggttatgact ccacttttat
     2641 cttttccaat tcctccaaat acatagatct taccatcaat aatagtagct gttgcctgct
     2701 ctctgggaac tgtcggccat tgcgctatct tagtccattt tttttcactc tggtttgtat
     2761 ctatcatata ccagttattt cccagacttc ctagtcctat atatatttta ccattctcga
     2821 tccctcctac accatattta aatggttcag gaatttccgg taaagctccg gctaccgacg
     2881 ttccaataaa caagcagaag ggaagatata taaagttttt cactttcatt gtaatcacct
     2941 tttaaaaatt aatcagaaca ccgacacggt aactattttc atgtttatta tctttcccct
     3001 tatacacccc ttgtttatcc aagtaatcat attctatata tggggtgaac accggtgaca
     3061 ttttatattg cagaacaaat gcattttcag ttgctgtttt tttatgattc ccatatttga
     3121 aattattaac cttcgtatat accgtggtct gccatgcaaa caaccatgaa tcattaattt
     3181 tgtatgtcac ataaccatca aactgatgct gatcattacg cgccctgttg ccatctaaat
     3241 ccgtctgtcg gaaagctttc cagtcatagc gatagcgtaa ggccagatta agatctggcg
     3301 ttgcctgata cgaaattttc agataagggt tatactgact tcctgctgac atccattgtg
     3361 tctgaatccc tgggattaga gcccatttgt catctatttt atatgtttta tttatatcta
     3421 ttttattata attgagtgca aacaattcat tttcattgtt gtgaattgta ttccaggtgt
     3481 ttgactcaaa acttgcccac caaccattat tccacccctg actaacctga atcaacgttt
     3541 cataagcgtg actggcgctt ctgtatcccc cacgtagatt aagagttaat gcctgagagc
     3601 acaaaggtaa accaagaaga attacagtta atagtggata atattttttc ataaatgcct
     3661 catcattaaa tatatttatc acttgtaatt tgatttctca atgaaactta tgttccgcaa
     3721 gccattccag aagcaggctt tgccattttt ctatcggata tccatttttt ccggatacac
     3781 caaatccatg acctccctta tcaaaaaaat gagcttctgc atgatgtccc ttatcccgaa
     3841 tagagttaaa atatctcaga gaatgaataa ctggaacatc cttatcatct gttgcatgca
     3901 caataaagac tggtggcata tactctgtaa cactattttc aagactggct gaatattcac
     3961 tttcacctgc atccagaaga cgtttccggc atccctgatg agcaatactc ctgtcaagag
     4021 atataactgg atatatcaga gccatccagt caggacgcac agaagtctct tcctcataat
     4081 ggagatgagt aatatatcca gcaagatgac cacctgccga aaaccccata catccaacac
     4141 tcttaaatgt tctctccgac attccggccc agtaagaaat aaagttcagg gcattcttcg
     4201 catctgcaaa ggaataataa ccattaacgg agggcattct gtaagaaaga ataaatgccg
     4261 tatatccctg acctgctatc catctgccaa catcaagtcc ctctctttca taaacaatcc
     4321 tcctgtatcc acctccaggt ataatcagca cactatgttg attagataca acaggctcaa
     4381 caactgtaat aaggggcaca atacctgtat atattatgcc atcgccagta ttctcaaata
     4441 gcgaactgtc agttatgtaa cgctcataat tatcaagaga atatatattc tccctgtaat
     4501 gaatcatttt tttattccct gatagcatcc gttattcttc tgcaacattc ttttttcttg
     4561 ctccataaaa aggttttcca tcgacagtat catattcaag cgcagcttct ggatggatcc
     4621 atctctgtac acgtgatggc atatcaaaac caataaggac aagaacaaca aatgttaaga
     4681 taaaagcaag agaacacatt gctgtaccaa gacttgtatg agacgcaaca actgcaccaa
     4741 gaataggcgc acatgctcca ccaagtgaac ctacattata aataaacccc agtcctgccg
     4801 cacgctgctc cacattaaaa taaccactga ttattttcgg caccagagca ccaatcccct
     4861 gaccaaaaac ctgctgagta aagattagaa ggccaagtaa ccatacatag tcccggtcca
     4921 caagaaaaac ggggataata acaagctgac cgataagaag tgatataaca tatgcttttc
     4981 gggttccaag ccagtccccc ataaaaccac ctatacaact tccagctgca gagccaaaac
     5041 ctgccagcat aaatatgaga gcaacagttt ctggtgaata ctgcatatca accttaagcc
     5101 atgtaggaag aaaagcctgc agtggccatc cgtagaggaa acagaacata accacaagca
     5161 tcaacataat gcctgttggc catcttttcc ccataccctg atatataaaa ctgataaaaa
     5221 cagccgcaca gcacaatgcc ataactgtga ttaaagcagc gtttgcagtg acgcctgaaa
     5281 aacaaacata tagacatgca aatgcaatac aagacagaag aatatttatg tatttattct
     5341 tttctctgta cagaatatcc accattgttc tgacgggttt attctcttgt tgcctttttt
     5401 tccagtcatc agactccgga agattcttgc gaagataaaa agcaaataat attggaagca
     5461 tgccgacaaa gaaaagagaa cgccagcccc atagtggtac tacaatgcta taaacctgtg
     5521 cagctaaacc tcccccaata gaaaatccag aaattaaaaa accacttgcc ttgtttctaa
     5581 gatgaacagg ccagctttcg ataacatatg ttacacttga gccatattca cctgccatac
     5641 caagcccaat aacaatgcga gcaataaaca tcactgcata tccaggagcc agaccacagg
     5701 caagggttcc gagagagaat agcactatac ttaaaaccat tgccatcctg cgccccatct
     5761 tgtccgataa agcgccaata gcaagccccc caaaccatcg agaaataaaa gcagcggaaa
     5821 taaggcttgc cgcttctatt gtcgtcaagc cgaattcatg ctgaacctct gtaagaacca
     5881 gagatatcag aacgaaatca aaaccatcaa gtaaataacc tgtccatgcc gcaccgaata
     5941 tcttccactg tctgggtgaa agatgcttat accaaacctg ctttttctga atacaggagt
     6001 ccatattaaa cctcacaata atagcggatt tataatacac tggagtatta ctttaagtaa
     6061 tacaatcccg gtcccaaaga gttgcaccaa taagaccaga gtcacttctg aaatatgccg
     6121 gaaacagcat tacattacaa atttcagaat atatggaaag atgtttcgac actttctgaa
     6181 catacccatc agcaagtcca acacttcctc cgacaacaac aacctgacaa tcaagcagta
     6241 attttaaact tactattaat tcagcaatag tactggcaga acgctctgtc aaaaattctg
     6301 cttccttata tccctgtctc gctaattcaa aaatatattt ggtgctatat cctgcaagtt
     6361 tattttttgc tgcccccatg atggcccgac cagatgaaac cgcttcaacg catccccgac
     6421 gaccacatcc acattctgta tccgttactc cggacaatat atgcccaaca tgtccggcta
     6481 atccagaaac acctgttaat aattttctgt taactacaat tccccctcca acccccgtag
     6541 aaacggttat aaaaaccata tcacaaattt ctttcggcaa aactgtatat tctgcccatg
     6601 cggctgcctg ggcatcattt atcacacttc catttaaacc agttatatct tccattatat
     6661 ttttcagagg atactctttc agccctccaa gattgtctgg attcagagct gtcaatactc
     6721 cattattaat aatccctgtt gatgctactg caaatcgctc cgctgttccc ttcatttcag
     6781 aaaccagtag acgtaaagcc cctgtcattt cgtcaggaca tctttcatga ggtgttgata
     6841 tctgtttttt cagcaaaaat gaaccgtcat cagatatcaa cgccgctgaa attttcgttc
     6901 ctcctatatc aacagccagt gttatcattc attttcacct atataacaat cttgcaatta
     6961 ttcagattat tttcattttt ttgtttctgt catgttcttc gaacagtcgc tctgcaagtt
     7021 ttgataatac atccagattc ttttccttta ccggagaaaa aggacttctg catacaggag
     7081 tatttataat ccccatatag taaagaagag tttttatacc tgccaggaca ccactctgaa
     7141 taagagtgtc aataacctgg ttacaggcta cttgcatttc cttcgcctta atcacatcat
     7201 tgtttttgac tgcttcaaat atttcaagat aacgccaccc cattatatta tatgtactac
     7261 cgattccccc atctgcacct gcaattaacc ctgatgcaaa tatttcatcg taaccattat
     7321 ataacacaag ctctggatga ttccttttga tttgttccat ctggaataaa tcaccggatg
     7381 tctgttttaa tgcacaaacc ctgggtattg tgacaagctc atttatctga tctaaagaaa
     7441 aacgcactcc gctaagagcc ggtatattat aaactaccat cggaagtcca tctgcagaat
     7501 caataatctt cctgtaatgg atacaatgct cttcaaatga aaaaggataa taaaatggag
     7561 taacagcaga tatagcatga tatccgtatt tttttgcagc ttttgcaagt acctcgcttt
     7621 ctgccgtact gattccgccc acatgcgcta taagtgtcag tcttccatct gattcatcgg
     7681 ctactgtttc aagaattttt tctcgctctg caacattctg aagaaatgct tctccagtag
     7741 agcctccaac atacaatccc ttaatatttt gttcgatatt aaaacggatc agttttcgaa
     7801 gactctccat atcgatttgc tgggattggt cataaggagt gggtaatgct gatatgaccc
     7861 ctttaaattc acattgcatt tttaatcacc ttcacttaac tgcataatgg gatacctagt
     7921 acaccatcaa gtacaaagat tatccaacag aaaagaagca caatgtgaag attatcaaaa
     7981 aaaaaccatc agaaagctgt catgccgctc agttaaggat cggttgatcg atccagtgtc
     8041 cagttactat tctctgcgta accctctgcg actatcttgc cccggaactg attacccaca
     8101 gccctgaaga gtccgacact gtcacgctac gcaaacggcg tcttcctctg gaaatgatgg
     8161 cctggtgaat tatcagtatg gcccatgagc gtaaggaacc ttcaccagat tgtgaaccgg
     8221 ctggacacat gctgccaggt agtcgcccct ttgtcgcgcc gtgatccatg cccagcagcg
     8281 gccaggaagc ggggccgtac gccacgtgtt cacgtaaaca gcgcagccct gacataacac
     8341 cacgctgtat ccgctctggt gcaatatgaa cctgctgacc atcgatggcg tgttctaact
     8401 aacacaggac acgccagaac acgacataaa gcttcactcg ccagatacat ggtaagcgta
     8461 aactgaccgc cgtatgtagc cattaagcca gtattggtaa cgtaggtgcc caccttttca
     8521 tcaactagtg gacacctgtt atggaacaga aaatattatc cgcagaaccc cgcagaacat
     8581 tttcaaatga gtttaaactt caaatggtta aactggcttc acaactagta gcctctgttg
     8641 cccgtattgc cagggaacac gatatcaatg ataacctcct gttcaaatgg ctcaggctct
     8701 ggcagaacga agggcgtata tcgcggcgtc ttccggtaac aacctcttct gacgctggcg
     8761 ttgaattatt acctgttgag ataacgcctg atgagcagaa agagcctatg gcggctctta
     8821 ctccgctttt atccactcct tcacaaagca cagtcagtgc cagttcctgt aaagttgagt
     8881 tccgccacgg taacatgacg ctggaaaatc cttcaccaga gctgctcacg gtgttgatcc
     8941 gtgaactgac cgggaggggg cgatgatctc gctcccatca gacactcgaa tctcgctggt
     9001 tgccggcgtt actgatatgc gtaaatcctt caacggtctg ggtgaacagg tacaacatgt
     9061 gctggatgag aaccccttct ccggtcacct gctcatcttc cgtggccgac ggagtgacat
     9121 gattaaaatc ctctgggctg atgctgatgg tctgtgcctg ttcaccaaac gcctggagga
     9181 aggcctgttt atctggcctg cggtgcgtga cggtaaggta tccattaccc gctcgcagct
     9241 ggcaatgctc ctcgataagc tggactggcg tcagccaaaa acatcccgtc ttaacgcact
     9301 gacaatgttg taaaaatgtc atggccggat tataaaaacg gtcatgagtc ggaaatacct
     9361 cattcgtatc actgagctgg aaaggctgct ctctgagcag gctgaagccc tccgtcagag
     9421 agatctgcaa ctgagtctgg ttgaagagac ggaggccttc ctgcgctctg cactggcacg
     9481 tgccgaagaa aagatcgaag aggaggagcg ggaaatagaa tatctgaggg cccagataga
     9541 aaaactgcgc cggatgctgt tcggtacccg ttctgaaaaa ctgcagcgtg aggttgaaca
     9601 ggctgaggcc caactgaaac aacgcgaaca ggaaagcgat cgttacagtg ggcgtgagga
     9661 tgacccgcag gttccccgcc agttgcgaca gtcacgtcat cgtcgtccgt taccagcaca
     9721 tcttccccgt gaaatacacc gcctggagcc tgaagaaagt tgttgcccgg agtgtggcag
     9781 tgagctggat tatctgggtg aagtcagtgc tgagcagctg gaactggtga gtagcgccct
     9841 gaaagtgatc cgcactgttc gggtaaaaaa agcctgtaca aaatgtgact gtatagttga
     9901 agcgccagcg ccgtcccgcc cgatagcgcg cggcatcgcg ggctccggat tacttgcccg
     9961 cgtgttaacg ggaaaatact gcgaacacct gccactgtat cgtcagagtg aaatctttgc
    10021 ccgacagggt gccgaactga gccgtgcatt aatctccaac tgggttgacg cgtgctgcca
    10081 gttaatgacc ccgctgaatg atgccctgta ccgttacgtg atgaacaccc gcaaggttca
    10141 cactgacgac acaccggtaa aagtgctgac accgggcaga aaaaaggcaa aaacaggacg
    10201 catctggacg tatgtccggg atgaccggaa tgcgggctca tcagagccac cggcggtctg
    10261 gttcgcctac tcaccagaca ggcagggaaa acatccggta caacaccttc gtcccttccg
    10321 gggtatcctg caggcggatg cattcagcgg ttacgatcgg ctgttcagtg ccaaacgtga
    10381 aggtggtgca cagacagaag ttgcgtgctg ggtccatgcc cggcgaaaaa tacacgatgt
    10441 atgaacgaca tctcttctga cgacattttc ctgctgaaac agcgcctggc cgaacaggaa
    10501 gtgctgatcc acgccctgca ggaaaagctg agcaaccggg agcgcgaaat agaccatctg
    10561 caggcgcagc tggataaact ccgccggatg aacttcggca gtcgttccga aaaagtctcc
    10621 cgccgtatcg cacaaatgga agccgatctg aaccggcttc agaaagagag cgatacgctg
    10681 actggtaggg tgtatgaccc ggcagtacag cgtccgttgc gtcagacccg cacccgtaag
    10741 ccgttccctg aatcactacc ccgtgacgaa aagcgactgt tgcctgcggc gccgtgctgc
    10801 ccgaactgcg gcggttcact gagctatctg ggcgaggata tcgccgaaca gctggagttg
    10861 atgcgtagcg ccttccgggt tatccggacg gtacgggaaa aacatgcctg tactcagtgc
    10921 gatgccatcg tgcaggcacc tgcaccttcg cggcccatcg agcggggtat cgccggaccg
    10981 gggctgctgg cccgcgtgct gacctcgaag tatgcagagc acaccccgct gtatcgccag
    11041 tcagaaatat acggccggca aggtgtggag ctgagccgtt cactgctgtc gggctgggtg
    11101 gatgcatgct gccggcagct gtctccgctg gaagaggcgc ttcatggcta tgtgctgact
    11161 gacggtaagc tccatgctga tgacacgcct gtcccggtgc tgttgccagg caataagaaa
    11221 acgaagaccg ggcggttatg gacctgcgtt cgtgacgacc gtaacgccgg gtcaacgctg
    11281 gcgccggcgg tgtggttcgc ttacagcccg gacagaaaag gcatccatcc gcagacccat
    11341 cttgcggggt tcagtggtgt actgcaggcg gatgcatacg ccgggttcaa cgagctgtac
    11401 cgggatggcc ggataacgga agccgcctgt tgggctcacg cccgccgtaa aatccacgat
    11461 gtgcacgttc gcaccccgtc agccctgacg gaggaagcgc tgaaacggat cggcgaactg
    11521 tacgccatcg aggcagagat aaggggaatg acggcggagc agcgccttgc cgaacgtcag
    11581 ttgaaaacga aaccgctgct gaaatccctg gaaagctggc tgcgtgaaaa gatgaaaacc
    11641 ctgtcgcgac actcagaact ggcgaaagcg ttcgcatacg ccctgaacca gtggccggcg
    11701 ctgacgtact atgcagatga tggctgggct gaggcggaca ataacatcgc tgaaaatgcg
    11761 ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct tcggttcgga tcatggagga
    11821 gagcggggag cgctgctgta cagcctgatc gggacgtgca aactgaacgg agtggagcca
    11881 gaaagctacc tccgctatgt ccttgacgtc atagccgact ggccgataaa ccgggtcggc
    11941 gaactgctcc cctggcgcgt agcactgccg actgaataac acatccccgt caatacggtt
    12001 cttgctgcac gcttaccctt ctgttgctca ctcagggcat gtaaatcatg ctcaaaccac
    12061 gccccctgag catctgtctg cagaatcaac cgaccatgac aggaaaggca gaaacaatgc
    12121 cggatatttc tgctgagcgc agagtcggca ctgataatgt gctcacccgg cgtgatcccc
    12181 agccccgttt ttataccgtt cattctgcta ccccctcatc actgaaacgc ccggtctggc
    12241 agtgtgtgca gtactgctcc ccataataat cgtggtgaca ttgcctgcag taccagctgg
    12301 ctttacgtac cacgggtaag gcatccggga cgaattgctg cagacgttta atcaaccgta
    12361 tttctctgcg ttccggcctg acataaggac attgctgcgc gtgctccgtc agcccgtcgt
    12421 cagtgtgttc aaaccagggc cgttcggtgt cgtattgcgg gtggtatctg agtgcactgc
    12481 cgcaaaggtg gcaggtgtag tgggcataag gagcagtctg tgcggtacgg gcaccggtca
    12541 gacgtccgtt gccatcaagt gcgagaaaag attttgcgta catagtatat gttcctctgc
    12601 gccagacgac acacaggcaa cagccgtccc ggtacgggca gcgtggtcag gtgtgaatgg
    12661 cggttttcag ttcagtctca gaagggaaaa tcagtgtggt cgctgtgaac acgtcatcac
    12721 actaaggtca gataagtcgt ctgtcgggct cagccacgac tgcagccaaa gttgcgctca
    12781 ccggtgccgt aa
//
LOCUS       DAOTRK010000129        12771 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.130, whole genome shotgun sequence.
ACCESSION   DAOTRK010000129 DAOTRK010000000
VERSION     DAOTRK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.130"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(249..2822)
                     /gene="clpB"
                     /locus_tag="QIE41_003634"
     CDS             complement(249..2822)
                     /gene="clpB"
                     /locus_tag="QIE41_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDS3532474.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2952..3683)
                     /gene="pgeF"
                     /locus_tag="QIE41_003635"
     CDS             complement(2952..3683)
                     /gene="pgeF"
                     /locus_tag="QIE41_003635"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDS3532475.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGRDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADISLLARQRLANVGVEQIFGGNRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3680..4660)
                     /gene="rluD"
                     /locus_tag="QIE41_003636"
     CDS             complement(3680..4660)
                     /gene="rluD"
                     /locus_tag="QIE41_003636"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDS3532476.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4795..5532
                     /gene="bamD"
                     /locus_tag="QIE41_003637"
     CDS             4795..5532
                     /gene="bamD"
                     /locus_tag="QIE41_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDS3532477.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5803..6144
                     /gene="raiA"
                     /locus_tag="QIE41_003638"
     CDS             5803..6144
                     /gene="raiA"
                     /locus_tag="QIE41_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDS3532478.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6247..6372
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6248..6295
                     /gene="pheL"
                     /locus_tag="QIE41_003639"
     CDS             6248..6295
                     /gene="pheL"
                     /locus_tag="QIE41_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDS3532479.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6394..7554
                     /gene="pheA"
                     /locus_tag="QIE41_003640"
     CDS             6394..7554
                     /gene="pheA"
                     /locus_tag="QIE41_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDS3532480.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7597..8718)
                     /gene="tyrA"
                     /locus_tag="QIE41_003641"
     CDS             complement(7597..8718)
                     /gene="tyrA"
                     /locus_tag="QIE41_003641"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDS3532481.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8729..9799)
                     /gene="aroF"
                     /locus_tag="QIE41_003642"
     CDS             complement(8729..9799)
                     /gene="aroF"
                     /locus_tag="QIE41_003642"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDS3532482.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10008..10373
                     /locus_tag="QIE41_003643"
     CDS             10008..10373
                     /locus_tag="QIE41_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDS3532483.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISA
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10523..11041
                     /locus_tag="QIE41_003644"
     CDS             10523..11041
                     /locus_tag="QIE41_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDS3532484.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11031..12257
                     /gene="dgcN"
                     /locus_tag="QIE41_003645"
     CDS             11031..12257
                     /gene="dgcN"
                     /locus_tag="QIE41_003645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDS3532485.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGSAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIVQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTACILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEVAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12273..12755
                     /locus_tag="QIE41_003646"
     CDS             12273..12755
                     /locus_tag="QIE41_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDS3532486.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
BASE COUNT         3307 a         3336 c         2976 g         3152 t
ORIGIN      
        1 cccgctacag agtcaagcat tttttgcttt tctctgttga gattctcagg agaacctcgc
       61 tgacccggcg gcgtgcttgc cgttgttccg tgtcagtggt ggcgcattat agggagttat
      121 tccggcctga caagcgaaaa atataaaaac tttatcgttc gctcattttt caggcaaaac
      181 atcttaaata tagtcttttc cgtctaactt atagacaaaa acgagccccg aagggctcgt
      241 tttatcattt actggacggc gacaatccgg tcttcattaa cttccaggcg aatcacttta
      301 cccggaacca attcaccaga cagtatttgc tgtgccagcg ggttttcaat ctgctgctga
      361 attgcacgtt tcagaggacg tgcgccatag accggatcgt aaccgttctc gctcagcagt
      421 ttcagcgcct cgtcagaaat gtggatttca taaccacgtt cttccagacg tttatacaga
      481 cgtttcaact gaatctgcgc aatcgacgca atgtgctgtt cacccagcgg atggaagacc
      541 accacttcat cgatacggtt aatgaattcc ggacggaagt tatggctaac cacaccgagc
      601 accagctctt tcatgtgcgc ataatccagt tcaccgaagc gttcctgaat cagatcggaa
      661 ccgaggttag aggtcataat gacgaccgta ttacggaagt cgaccgttct cccctgcccg
      721 tcagtcagac gcccatcatc cagtacctgc aacagaatgt tgaagacatc cggatgcgct
      781 ttttccactt catccagcag gataacggaa tacggacgac gacgcacagc ttcggtcagg
      841 tagccacctt cttcataacc aacatatccc ggaggcgcac caaccagacg agacaccgaa
      901 tgtttctcca taaactcgga catatcgata cggaccatcg cctcgtcgct atcaaacata
      961 aagttcgcca gcgccttaca aagctcagtt ttccccacac ccgttgggcc gaggaacagg
     1021 aatgaaccaa tcgggcgatt tggatccgcc agccccgcac ggctacgacg aatagcgtta
     1081 gataccgcat caaccgcttc gttctgacca ataacgcgat ggtgcagttc ttgctccata
     1141 cgcagcagtt tttcgcgctc gctttccatc atgcgagaaa ccggaatccc cgtccaacgc
     1201 gccagcactt cagcaatttc ggcatcggtc actttattac gcaacagacg catagttttg
     1261 ccttcgagct gcgttgcggc ttccagttgc ttttccagtt ccgggatttt gccgtattgc
     1321 agttcagaca tccgcgccag gtcacccaca cggcgagcct gttcaatagc gattttcgcc
     1381 tgttccagtt ccgctttaat ggtctgcgta ccagaaagcg atgctttctc tgctttccac
     1441 tcttcttcta actcggagta ctgacgttct ttgtcgctca gttcttcgtt gagcatatcc
     1501 aggcgttttt tactggcttc atcagactct ttcattaacg cctgttgttc cagtttgagc
     1561 tggatgatac gacgatcgag tcggtcgagt tcttctggtt ttgagtcaat ctgcatacga
     1621 atgctggatg ctgcttcatc gatcaggtcg atggctttat caggcagctg acggtcagca
     1681 atgtagcgat gagacaacgt cgccgctgca acaattgccg ggtcagtaat ttgcacatgg
     1741 tggtgcaatt cgtaacgttc tttcaggcca cgcagaatcg caatggtatc ttcaacagaa
     1801 ggctcggcaa caaacacttt ctggaaacga cgttccagcg cagcatcttt ttcgatgtac
     1861 tggcgatatt cgtcaagcgt cgtggcacct acgcagtgca attcaccacg cgccagcgcc
     1921 ggtttcagca tgtttccggc gtccattgcg ccatcggctt tacccgcgcc gaccatggta
     1981 tgtaattcgt cgataaacag gatgacgttg ccttcctgtt tagcaagatc gttaagtacg
     2041 ccttttaaac gttcttcaaa ctcaccgcga tatttcgccc cagccaccag cgcgcccata
     2101 tccagcgcca gtacgcggcg gcctttcaac ccttccggca cttcgccgtt gataatacgc
     2161 tgcgccagac cttcaacgat ggcagtttta ccgacgccgg gttcaccaat cagtactggg
     2221 ttatttttag tacgacgttg cagcacctga atggtacggc gaatttcttc atcacgacca
     2281 atcaccggat cgagtttgcc ctgttcggct cgttcggtaa ggtcgatggt atattttttc
     2341 aaagcctgac gttggtcttc agcaccttga tcgttcacgc tttcacctcc acgcatttgt
     2401 tcaatcgctt gagtaatgtt ggcggtggtc gcccctgctg ctttcaggat gtcggccagc
     2461 gtgccgcgag actcaagtgc cgccagaacg aacagttctg acgagataaa gttatcacca
     2521 cgtttttgcg ccagcttgtc gcaaagatta agaacgcgca ccagatcttg tgatggctgg
     2581 acatcaccac cagtaccttc aacctgcggt aaacgattta atgcctgatt gatatctgtg
     2641 cgcaactggc cagcatttat gccagcggat gttaataaag gactaaccga acccccttcc
     2701 tgattcagca gggcgctcat taaatgaagt ggttcgatga attggttgtc gtgcccgagt
     2761 gcaagtgatt gggcatcggc aagagcaagc tggaatttat tagtaagacg atccagacgc
     2821 ataactcctc ccataacgga tcaaagttgc tactggagat taaatgaggt catccctcaa
     2881 ttattcaagg ttattgacca gattaatgtg aaaagaaaat cacgcgtacc ggatcgtctt
     2941 gattctttag gttatatcag ccaaatgaaa cttgccatac gaccggtggt cttgtcgcga
     3001 cgataagaga agaaagtctc attttccgta tatgtacaac ggttgccgcc gaaaatttgt
     3061 tcgacaccga cgttcgccag acgctgccgg gcaaggagag aaatatccgc cagatactta
     3121 tcaccatgct gaatgaaagc tgtactcgct ttggcgtcta ctgccataaa cgcctcgcga
     3181 acctccgccc ccacttcaaa cgcgcgtgga ccaattgccg gccctaacca ggcgagaata
     3241 ttttccggat tatcagcaaa acaggaaacc gtctcctcca acacgccagc gcacagtcca
     3301 cgccagccag catgagcggc ggcgacttcc gttcccgctc gattgcaaaa cagcacgggg
     3361 aggcagtcgg cagtcatcac tgcgcaaacc gtgcccggcg tgttgctata agaggcatct
     3421 gctcgttttg aggcataagg ttcgccagta agcttaagca catctctgcc gtgtacctgc
     3481 tcaagccaga ccggttttga aggcaaattg cccgcagcaa aaagtcgctt gcgattctcc
     3541 tcaacgtgat ccgggttatc gccacaatga gcaccgaggt tgagcgagtc atacgggggc
     3601 aagctcacgc cgccgatacg agtagagcta caggccgcaa caccttttgg caggggccac
     3661 tgcgggacaa tcagcttact cataaccagt ccacttcatc tttatgctct tcgaaatcgg
     3721 cgcgcatcac ctcaatcagc tccaccatat cttgtggaat aggcgcatgc cattccattt
     3781 cgatgccgga gattgggtga taaagacgca gcatggttgc atgaagcgcc tggcggtcaa
     3841 acttacgcag cgtggagata aacgcttccg aagcaccttt tggcggacgc ggacggccac
     3901 cataaaccgg atcgcccacc agcggatgag tgatatgggc catatgcacg cggatctggt
     3961 gcgtacgtcc agtttccaga cgcaaccgca gacgcgtgtg cacacggaag tgttccatga
     4021 tgcgatagtg agtgaccgcc ggtttgccca tgggatgcac cgccatgtga gtacgtttgg
     4081 tcgggtggcg gctgattggt tcatctactg taccgccagc ggtcatatga ccaattgcca
     4141 ccgcttcata ctcacgagta atttcacgcc gttgcaaaga ttcgactaaa cgcgtctgag
     4201 ccggaacggt ttttgccaca accatcaggc cggtggtgtc tttatccaga cgatggacga
     4261 tgcccgcacg cggtacatcg gcaatgggcg gatagtaatg aagtaacgca ttcagtaccg
     4321 taccatccgg gttacccgcg ccaggatgta ccaccaggtc gcgtggttta ttaatgacga
     4381 tgatatcttc atcttcatag acgatatcca gcgggatatc ctgcggttca aaacgcgctt
     4441 cttcttcaat ctcagcgttg atggccacct gctcgccacc caatactttt tctttcggct
     4501 tatcacaaac tttgccgtta accagcacgc gctggtcgag gatccattct tttatccgcg
     4561 aacgtgaata atccgggaac atttcggcca aagcctgatc taagcgttga ccgagttggt
     4621 tttcggacac cgttgcagtg agctgtactc gttgtgccat atatactgct tcttcgttta
     4681 tcgttggggt tttacggctt tgccgtttaa tatagtgtgc tattgtagct ggtcttaacc
     4741 gggagcagga acagagaatc tcccgtatta cattttgagg aaagtcaaaa cgtcatgacg
     4801 cgcatgaaat atctggtggc agccgccaca ctaagcctgt ttttggcggg ttgctcgggg
     4861 tcaaaggaag aagtacctga taatccgcca aatgaaattt acgcgactgc acaacaaaag
     4921 ctgcaggacg gtaactggag acaggcaata acgcaactgg aagcgttaga taatcgctat
     4981 ccgtttggtc cgtattcgca gcaggtgcag ctggatctca tctatgccta ctataaaaac
     5041 gccgatttgc cgttagcaca ggctgccatc gatcgtttta ttcgccttaa cccgacccat
     5101 ccgaatatcg attatgtcat gtacatgcgt ggcctgacca atatggcgct ggatgacagt
     5161 gcgctgcaag ggttctttgg cgtcgatcgt agcgatcgcg atcctcaaca tgcacgagct
     5221 gcgtttagtg acttttccaa actggtgcgc ggctatccga acagtcagta caccaccgat
     5281 gccaccaaac gtctggtatt cctgaaagat cgtctggcga aatatgaata ctccgttgcc
     5341 gagtattata cagaacgtgg tgcatgggtt gccgtcgtta accgcgtaga aggcatgttg
     5401 cgcgactacc cggataccca ggctacgcgt gatgcgctgc cgctgatgga aaatgcatac
     5461 cgtcagatgc agatgaatgc gcaagctgaa aaagtagcga aaatcatcgc tgcaaacagc
     5521 agcaatacat aacaaaaacc tgaaacacaa aacggcagcc cttgagctgc cgttttttta
     5581 ttctgttagt tgtgaaactg aagcgattta gtcactatcg atctcatcaa atatggctcg
     5641 ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc gatttctcac aaaaaagatt
     5701 cgttgacaaa aagtgacaaa attatgagat ttccatcaca cattttgaca tcaggaacgg
     5761 tatgctgaat tcaccaagac gggaagacaa gaggtaaaat ttatgacaat gaacattacc
     5821 agcaaacaaa tggaaattac tccggccatc cgccaacatg tcgcagaccg tctcgccaaa
     5881 ctggaaaaat ggcaaacaca tctgattaat ccacatatca ttctgtccaa agagccacaa
     5941 gggtttgttg ctgacgccac aatcaataca cctaacggcg ttctggttgc cagtggtaaa
     6001 catgaagata tgtacaccgc aattaacgaa ttgatcaaca agctggaacg gcagctcaat
     6061 aaactgcagc acaaaggcga agcacgtcgt gccgcaacat cggtgaaaga cgccaacttc
     6121 gtcgaagaag ttgaagaaga gtagtccttt atattgagtg tatcgccaac gcgccttcgg
     6181 gcgcgttttt tgttgacagc gtgaaaacag tacgggtact gtactgaagt cacttaagga
     6241 aacaaacatg aaacacacac cgtttttctt cgcattcttt tttaccttcc cctgaatggg
     6301 aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa taaggcctcc caaatcgggg
     6361 ggcctttttt attgataaca aaaaggcaac actatgacat cggaaaatcc attactggcg
     6421 ctgcgagaga aaatcagcgc gctggatgaa aaattattag cattactggc agagcggcgc
     6481 gaactggccg tcgaggtggg aaaagccaaa ttgctctcgc atcgcccggt acgtgatatt
     6541 gatcgtgaac gcgatttact ggaaagatta attacgctcg gtaaagcgca ccatctggac
     6601 gcccattaca ttactcgcct gttccagctc attattgaag attccgtatt aactcagcag
     6661 gcgttgctcc agcaacatct caataaaatt aatccgcact cagcacgcat cgcttttctc
     6721 ggccccaaag gttcttattc ccatcttgcg gcgcgccagt atgctgcccg tcactttgag
     6781 caattcattg aaagtggctg cgccaaattt gccgatattt ttaatcaggt ggaaaccggc
     6841 caggccgact atgccgtcgt accgattgaa aataccagct ccggtgccat aaatgacgtt
     6901 tacgatctgc tgcaacatac cagtttgtcg attgttggcg agatgacgtt aactatcgac
     6961 cattgtttgt tggtctccgg cactactgat ttatccacca tcaatacggt ctacagccat
     7021 ccgcagccat tccagcaatg cagcaaattc cttaatcgtt atccgcactg gaagattgaa
     7081 tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac aggcaaaatc accgcatgtt
     7141 gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt tgcaggtact ggagcgtatt
     7201 gaagcgaatc agcgacaaaa cttcacccga tttgtggtgt tggcacgtaa agccattaac
     7261 gtgtctgacc aggttccagc gaaaacgacg ttgttaatgg cgaccgggca acaagctggt
     7321 gcgctggttg aagcgttgct ggtactgcgc aaccacaatc tgattatgac ccgtctggag
     7381 tcacgcccga ttcacggtaa tccgtgggaa gagatgtttt atctggatat tcaggccaat
     7441 cttgaatcag cggaaatgca aaaagcattg aaagagttag gggaaatcac ccgttcaatg
     7501 aaggtattgg gctgttaccc aagtgagaac gtagtgcctg ttgatccaac ctgatgaaaa
     7561 ggtgccggat gatgtgaatc atccggcgct ggattattac tggcgattgt cattcgcctg
     7621 acgcaataac acgcggcttt cactctgaaa acgctgtgcg taatcgccga accagtgctc
     7681 caccttgcgg aaactgtcaa taaacgcctg cttatcgccc tgctccagca actcaatcgc
     7741 ctcgccgaaa cgcttatagt aacgtttgat taacgccaga ttacgctctg acgacataat
     7801 aatgtcggca taaagctgcg gatcctgagc gaacagtcgc ccgaccatcg ccagctcaag
     7861 gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc tgaacatttt cttctgccag
     7921 gtgcagcccg taagcaaaag tagcaaagtg gcgcagtgcc tgaataaacg ccatattctg
     7981 atcgtgctcg acggcgctaa tacgatgcaa ccgagcgccc cagacctgaa tttgctccag
     8041 aaaccattgg tatgcttccg gcttacgtcc atcacaccag accacaactt gctttgccag
     8101 gctaccgctg tccggaccga acattgggtg tagacccagt accgggccat cgtgcgccgc
     8161 cagcatggcc tgtaatggtc catttttcac cgatgccaga tcaaccagaa tacaatcttt
     8221 cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg tggattggca cactaacaat
     8281 caccattccg gcatcggcaa caatatcagc cgctcgatcc cagtcatgtt gctccagaat
     8341 ccgcacctga taacccgata gtgtcagcat cttctcgaac aggcgtccca tctgaccgcc
     8401 accgccgacg ataaccactg gacgcagtga cggacaaagt gttttaaatc ctttgtcgtt
     8461 ttcactggag taagattcac gcatcacccg acgcaaaaca tcctcaatca gatctggcgg
     8521 tacacccaga gcttccgcct ctgcacgacg cgaggccaac atagatgcct cgcgctccgg
     8581 aacataaata ggcagtccaa agcggctttt cacctcgccc acttcagcaa ccagttccag
     8641 acgcttcgct aataaattca gcagcgcttt atcgacttca tcaatttgat cgcgtaatgc
     8701 ggtcaactca gcaaccataa taaacctctt aagccacgcg agccgtcagc tgcccgttca
     8761 gatcctgatg aatttcacgc agcaaggcat cggtcatttc ccaactaatg caggcatcgg
     8821 ttacggatac accgtatttc atttcactgc gcggttgctc ggaagactga ttgccctcgt
     8881 ggatattact ttcgatcatc agaccaataa tcgagcgatt gccatctttg atctgagcaa
     8941 ccacggactc tgccaccgca ggctgacggc gataatcttt attggaatta ccgtggctgc
     9001 aatctaccat cagagacggg cgcagtcccg cctgttccat ctctttttca cattgcgcaa
     9061 catccgcagg gctatagttc ggcgctttac caccgcgcag gatcacatgc ccgtccggat
     9121 tcccctgagt ttgtagcaac gccacctgcc ctgcctggtt aatgccaaca aaacggtgcg
     9181 gctgggcggc ggcgcgcata gcgttaattg ctgttgccag actgccgtcg gtgccgtttt
     9241 taaaaccaac cggcatggaa agcccggagg ccatttcacg gtgagtttgc gattccgttg
     9301 tacgagcacc aattgctgac cagctaaaca gatcgcccag gtattgcggg ctatttggat
     9361 ctaacgcttc cgtcgccagt ggcagtccca tattcaccag ctcaagcagc aatttacgcg
     9421 cgatctgcag cccggcttct acatcaaaag agccatccat atggggatcg ttaattaacc
     9481 ctttccagcc gacagtggta cggggttttt caaaatagac gcgcattacc agatagaggc
     9541 tatcgctgac ctctgcggca agggctttaa atcgacgagc atattccaga gcagtttccg
     9601 gatcatgaat ggaacaagga ccacatacta ccaacagacg aggatcgcgc ccggcgataa
     9661 tatctgaaat ggttttacgc gagtcagcaa tctgggcttc ttgttgcagg ctcaatggaa
     9721 aagcggcctt cagttgttcc ggagtcatta aaacctgttc gtcggtaata tgtacgttat
     9781 tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct cgtttgcgat agttgatcct
     9841 cagcgaggat gacgtaacga taacacataa agtaaagttt tcaatccata tttcgtacat
     9901 ttttctttac agaaacaatt tactcgcgct tttgatgctt atttcgtaca aataaattta
     9961 cacctccttt aaaaacaatc cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt
    10021 ttatcgcccc cttgttggct ttactggtta gcggatgtca gattgatcct tatactcacg
    10081 cgccaacctt gaccagcacc gactggtatg atgtcggtat ggaagatgcg atatcggcca
    10141 gcgccataaa agatgacgat gcatttagcg attcacaggc ggatcgcggt ctatacctta
    10201 aaggatatgc cgaaggacaa aagaaaactt gccagaccga ttttacttat gcccggggcc
    10261 tttccggtaa aagctttcct gccagctgca acaatgttga aaatgccagt caactgcatg
    10321 aagtatggca aaaaggggct gatgaaaatg ccagcacaat acgattaaat taattaaccc
    10381 cccacaaata aattaaacat gagattctac tcattgtgtt ttatttctca cattgatgac
    10441 gaccgcatga aagaatgata ttttaatgag cattctgaaa aaatatcgat gcatttcgag
    10501 cgaagatggt gaggatccct gaatgcgttt ttctcaccga ctgttcttac tgctgattct
    10561 cctgctgacg ggagcaccaa tactcgctca ggaaccttct gacgttgcga aaaacgtgcg
    10621 tatgatggtt tccggtattg tcagttacac ccgctggcct gcgctatcag ggccaccaaa
    10681 gttatgtatc ttttcatctt cacattttag taacgcccta caagagaatg gtacgacatc
    10741 tttgccttat ctgcctgtca tcattcacac tcaacaagag gcgatgattt caggctgcaa
    10801 tggtttttat tttggcaacg agtcaccgac attccagatg gaattaaccg aacaatatcc
    10861 gtcaaaggcg ttattattaa ttgccgaaca aaataccgag tgcattattg gtagcgcctt
    10921 ttgtctgatc atccacaata atgacgtcag atttgccgta aacctggatg ccttatcgcg
    10981 tagcggtgta aaggtcaacc cggatgtctt gatgctcgca cggaagaaaa atgatggata
    11041 acgataattc tcttaataag cgccccacgt ttaaaagagc attacgcaac atcagtatga
    11101 ccagcatatt tatcactatg atgctgatct ggttgctgct ttccgtgacc tcggtgctga
    11161 ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc agcaacaatg acttacagtc
    11221 tggaagcagc ggtcgttttt gccgatggct ctgcagcaac tgaaacactg gcagcgctgg
    11281 ggcagcaagg ccaattttca actgcagaag tgcgtgataa gcagcaaaat attctggcat
    11341 cctggcatta cacccgtaag gatccaggcg atactttcag taatttcata agccactggc
    11401 tcttccctgc ccccatcgtt cagccgattc gtcacaatgg tgaaaccatt ggcgaagttc
    11461 gcttaaccgc tcgcgacagt tcaatcagcc atttcatctg gttttcgctc gctgtcctga
    11521 ccgcctgcat tctgctggca tcaggcatcg caattaccct cacccgccat ttgcacaatg
    11581 gtctggttga agcgttaaaa aacatcaccg atgtcgtaca tgatgtgcgt tccaaccgca
    11641 atttttcccg acgagtttcg gaagaacgta tcgcagagtt tcaccgcttc gctctcgact
    11701 tcaacagtct gctggatgaa atggaagagt ggcagcttcg tttacaggcc aaaaatgcgc
    11761 agcttctgcg taccgcgcta catgacccat taaccgggct ggctaaccgc gcagcgtttc
    11821 gtagcggtat caatacgttg atgaacaatt ccgatgcccg aaaaacgtcg gcgttactat
    11881 ttcttgatgg cgataatttc aaatatatca atgatacctg gggtcatgcg acgggcgatc
    11941 gtgtcttgat tgaggtagca aaacggttag ctgaatttgg cgggctgcga cataaagcat
    12001 accgcctggg cggcgatgaa ttcgcgatgg tgctctatga tgtacagtcg gaatctgaag
    12061 tgcagcagat atgctcagca ctgacacaaa tctttaatct cccgtttgat cttcataatg
    12121 gccatcagac caccatgaca ttaagcattg gttacgcgat gaccattgag cacgcctctg
    12181 cggaaaaatt acaagagctt gccgatcaca atatgtatca ggccaaacac cagcgtgccg
    12241 aaaagctggt gagataacaa ggatatatcg atatgataaa gcacctggta gcacccctga
    12301 ttttcacctc actaatactg actggctgcc agtcccctca gggaaagttt actcctgagc
    12361 aagtcgccgc tatgcaatct tatggattta ctgaatccgc cggcgactgg tcgctgggct
    12421 tatcagatgc cattctgttc gcaaaaaatg actacaaatt gctcccggaa agccagcaac
    12481 agatccaaac catggcagcc aaattggcct cgacagggct aacacatgcc cgtatggatg
    12541 gacacaccga taactatggt gaagatagtt acaacgaagg cttatcattg aaacgggcga
    12601 atgtcgtggc cgatgcatgg gctatgggtg gacaaattcc gcgcagcaat ctcaccacac
    12661 agggtttagg aaaaaaatat cccatagcca gtaacaagac cgcccagggc cgcgccgaga
    12721 accgccgcgt cgcagtggtg attactaccc cttaaaacac gagcaaacag a
//
LOCUS       DAOTRK010000130        12592 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.131, whole genome shotgun sequence.
ACCESSION   DAOTRK010000130 DAOTRK010000000
VERSION     DAOTRK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.131"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            167..541
                     /gene="tomB"
                     /locus_tag="QIE41_003647"
     CDS             167..541
                     /gene="tomB"
                     /locus_tag="QIE41_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDS3532487.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            567..785
                     /gene="hha"
                     /locus_tag="QIE41_003648"
     CDS             567..785
                     /gene="hha"
                     /locus_tag="QIE41_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDS3532488.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            957..1508
                     /gene="maa"
                     /locus_tag="QIE41_003649"
     CDS             957..1508
                     /gene="maa"
                     /locus_tag="QIE41_003649"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDS3532489.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1624..2094
                     /locus_tag="QIE41_003650"
     CDS             1624..2094
                     /locus_tag="QIE41_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDS3532490.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2258..3808
                     /gene="pdeB"
                     /locus_tag="QIE41_003651"
     CDS             2258..3808
                     /gene="pdeB"
                     /locus_tag="QIE41_003651"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDS3532491.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(3850..4203)
                     /locus_tag="QIE41_003652"
     CDS             complement(3850..4203)
                     /locus_tag="QIE41_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HDS3532492.1"
                     /translation="MKYVDGFVVAVPVDKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4323..4419)
                     /gene="ffs"
                     /locus_tag="QIE41_003653"
     ncRNA           complement(4323..4419)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="QIE41_003653"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4582..4893
                     /locus_tag="QIE41_003654"
     CDS             4582..4893
                     /locus_tag="QIE41_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDS3532493.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(4924..5496)
                     /locus_tag="QIE41_003655"
     CDS             complement(4924..5496)
                     /locus_tag="QIE41_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDS3532494.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5714..6574
                     /gene="tesB"
                     /locus_tag="QIE41_003656"
     CDS             5714..6574
                     /gene="tesB"
                     /locus_tag="QIE41_003656"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDS3532495.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6623..7909)
                     /gene="amtB"
                     /locus_tag="QIE41_003657"
     CDS             complement(6623..7909)
                     /gene="amtB"
                     /locus_tag="QIE41_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDS3532496.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(7939..8277)
                     /gene="glnK"
                     /locus_tag="QIE41_003658"
     CDS             complement(7939..8277)
                     /gene="glnK"
                     /locus_tag="QIE41_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDS3532497.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADESAL"
     gene            complement(8458..10239)
                     /locus_tag="QIE41_003659"
     CDS             complement(8458..10239)
                     /locus_tag="QIE41_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDS3532498.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVSM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQSRFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10232..12004)
                     /locus_tag="QIE41_003660"
     CDS             complement(10232..12004)
                     /locus_tag="QIE41_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDS3532499.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGTANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12034..12492)
                     /gene="decR"
                     /locus_tag="QIE41_003661"
     CDS             complement(12034..12492)
                     /gene="decR"
                     /locus_tag="QIE41_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDS3532500.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
BASE COUNT         3173 a         3399 c         3033 g         2987 t
ORIGIN      
        1 cctgtcccga ttccttccta attgtaattt ttcgtaataa tgcgatgaaa acctgcaaag
       61 agtggcttat agttaagcta acaaacgaga gggcaagtcc aggtcagtaa gttttttcca
      121 tcccgaaagg tgtccgttag ttcaaccact aagaagggga cgcgttatgg atgaatactc
      181 acccaaaaga catgatatcg cacagcttaa gtttctctgt gaaaccctgt atcatgactg
      241 tcttgcaaac cttgaagaaa gcaatcatgg ctgggttaac gacccaacct cggcgatcaa
      301 cctccagttg aatgaactga ttgagcatat tgcgaccttc gcacttaatt acaaaattaa
      361 gtataatgaa gacaataagc tcattgagca gatcgacgaa tatctggatg acacctttat
      421 gttgttcagc agttatggta ttaatatgca ggatcttcag aaatggcgga agtcaggtaa
      481 tcgactattc cgttgttttg tcaatgcgac gaaagagaat cctgcgagtt tatcttgtta
      541 gaattattac aaccatgggt agaagtatgt ccgaaaaacc tttaacaaaa accgattatt
      601 taatgcgttt acgtcgttgc cagacaattg acacgctgga gcgtgttatc gagaaaaata
      661 aatacgaatt atcagataat gaactggcgg tattttactc agccgcagat caccgcctcg
      721 ccgaattgac catgaataaa ctgtacgaca agatcccttc ctcagtatgg aaatttattc
      781 gctaataaat aattcgcttt cggagctata accggctgtt tattaagaat tttatacttt
      841 ttcgccatga agacgtaccc tatgtgatct ttatcacaca gatgtaatgg gaacgttctc
      901 ttcactgact tttcgtctta ctgtgttgcc gcattttcag caaccggagg tcagtaatga
      961 gcacagaaaa agaaaagatg attgctggtg agttgtatcg ctcggcagat gagacgttat
     1021 ctcgcgatcg cctgcgcgct cgtcagctta ttcaccgata caatcattcc ctggcggaag
     1081 agcacacatt acgccagcaa attctcgctg atttattcgg tcaggtgaca gaggcttata
     1141 ttgagccaac gtttcgctgt gattatggct ataacatttt tctcggcaat aattttttcg
     1201 ccaacttcga ttgcgtgatg cttgatgtct gccctattcg catcggtgat aactgtatgt
     1261 tggcaccagg cgttcatatc tacacggcaa cacatcccat cgaccctgta gcacgtaata
     1321 gcggtgctga actggggaaa cccgtcacca tcggtaataa cgtctggatt ggcggacgcg
     1381 cggtcattaa ccctggtgtg accattggtg ataacgtcgt ggtagcctca ggtgcagttg
     1441 tcacaaaaga tgtcccggac aacgttgtcg tgggcggtaa tccagccaga ataattaaaa
     1501 aattgtaata ggtttttcgc aactgttatg caaaattgtg gtaaatctgt tactttccct
     1561 ctactattcc cacgttaaaa tagggtgttc cctggaaagt tgcagatacc acgaaggcaa
     1621 acgatgaccg aaatacaacg cctgctgacc gaaacaattg agtctctaaa tacccgcgaa
     1681 aaacgcgaca acaaaccccg ctttagtatc agttttatcc gtaaacatcc ggggctgttt
     1741 atcggtatgt atgttgcttt ttttgccacc ctggcggtga tgttgcagtc cgaaacgctt
     1801 tcaggctctg tctggctact ggttgtatta tttatcctgc ttaatggttt cttctttttc
     1861 gatgtctacc cacgctaccg ctatgaagat atcgacgtgc tggatttccg cgtttgctat
     1921 aacggcgaat ggtacaacac gcgctttgta cctgccgcgc tggttgaagc catcttgaac
     1981 tctccgcgtg tcgcggatgt tcataaagaa caactgcaaa aaatgatcgt ccgtaaaggt
     2041 gaactgtctt tttacgatat ttttaccctc gctcgcgccg aatcaacatc ttaagttagg
     2101 gttacatacc aggcgtaaag ctctgcgcct ggtcaaatga caacgttcgt ttccacccat
     2161 cccttcatga aatcccggct ctacctcctt atctcctgca agcctttttc cacaatcaga
     2221 tatactttcc ctacactgtg ttaataagga tatggtggtg agaacacgac atctggtcgg
     2281 ccttatttcg ggagtactga ttctttcagt attgctgcct gtcggcttaa gcatctggct
     2341 ggcccatcag caggtagaaa catcatttat tgaagagctg aatacgtatt cctcccgcgt
     2401 cgctattcgg gccaataagg tggcgacaca agggaaagat gcgctgcaag agctggaaag
     2461 atggcaaggc gctgcctgta gcgaagccca tctcatggaa atgcgtcggg tatcttacag
     2521 ttatcgctat attcaggaag tggtttatat cgataacaac gttccccagt gttcgtctct
     2581 ggagcatgaa agcccgcccg ataccttccc cgagccaggt aaaatttcga aagatggtta
     2641 tcgtgtctgg ttaacatcgc ataacgattt aggcattatc cgttacatgg ttgccatggg
     2701 aacggcacat tatgtcgtca tgatcgaccc cgcttccttt attgatgtca ttccctatag
     2761 ctcatggcaa attgatgccg ccattattgg caatgcccat aacgttgtga taaccagcag
     2821 cgatgaaatt gctcagggaa ttattaccag actacaaaaa acacccggtg agcatatcga
     2881 aaataatgga atcatttacg atatcctgcc cttcccggaa atgaatattt cgatcatcac
     2941 ctgggcttca acgaaaatgt tgcagaaagg ctggcatcgg caagtcttta tttggttacc
     3001 gctcgggttg gtgattggcc tgctggcagc gatgtttgtg ctgcgtattt tgcgccgtat
     3061 ccagtcaccg catcatcggc tgcaggatgc tatcgaaaat cgtgatattt gcgtgcacta
     3121 tcagccgatt gtctccttag ccaatggcaa aattgtcggt gctgaggcac tggcgcgctg
     3181 gccgcagaca gacggtagtt ggttgtcacc agatagtttt attccgctgg cacagcaaac
     3241 gggcctttct gagccattga cgctactgat tataagaagc gtctttgaag atatgggcga
     3301 ctggctgcgt cagcatccgc agcagcatat ttcgatcaat cttgaatcca ccgtgctcac
     3361 ctcggaaaaa atcccgcaat tgctgcgtga aatgatcaat cactatcagg ttaatcccag
     3421 acagatcgcg cttgaactca ctgaacgcga gtttgccgat ccgaaaacca gcgccccgat
     3481 aatttctcgc taccgggagg cgggccatga aatttatctt gatgattttg gtacggggta
     3541 ttcaagttta agttatttac aggatctgga tgtcgacatt ctgaagatcg ataaatcttt
     3601 cgttgatgcg ctggaatata aaaatgtcac gccgcatatc atcgaaatgg caaaaacact
     3661 gaaactgaaa atggtagcgg agggaatcga aaccagtaaa caagaagagt ggttacgcca
     3721 gcatggcgtg cactacggtc agggctggct ctacagcaag gcattaccga aagaagattt
     3781 cttacgctgg gccgagcaac atttgtgaat tgtagggctg acggcacgcc gcacagccca
     3841 tcacgctacc tattcgtcga tgattgactc gaatccgcca tagatcattc gcttaccatc
     3901 aaacggcatg gactcgccga actctttcat ccgtgggtcc gacatcatct tttgattagc
     3961 agcgtcgcgg acctcttttg aagggtattc aatccagcta aagacaacct cttcattctc
     4021 ttcagctttt accgccatac gaaaatcggt cactttgcca tccggtacat cgctggccca
     4081 gcattcgaca atacgaagcg cgccaaactc tttaaacaat ggcgcagcct tagcagccat
     4141 ttcccgatag gcatcctttt tatcgacagg aacggcaacc acaaaaccat caacatactt
     4201 catcagacat acctccgaag cgtactgcgc agccagacaa ttcttgcgac gtcagttgtg
     4261 gttaaagttt agccatattg ggattttttg acgaaacggg gggaggcaga aatgggtggg
     4321 ggccctgcca gctacatccc ggcacacgcg tcatctgcct tggctgcttc cttccggacc
     4381 tgacctggta aacagagtag cgttgcggga gaaccaacag agcccccatt gagagcgttg
     4441 agaaccaacg cgcaggcgca ttatcactgc tgccccggct aattgcaacc cattgcgtgc
     4501 cggatgctgg tttcttgcgc aatgcggctt cactcgggcg tttttccaga ttatgctgaa
     4561 aaattacctc aggaagaaaa gatggaaaaa gaagattcat ttccccaacg cgtctggcaa
     4621 atcgtcgccg ctattcccga aggctatgtc accacttacg gtgatgtggc gaaactggca
     4681 ggatcgcccc gcgccgcgcg ccaggtgggc ggtgtgttaa agcgtctccc cgaaggcagc
     4741 accttaccct ggcaccgggt ggttaatcgc cacggcacaa tttcgctaac cggaccggat
     4801 ttacagcgtc aacgacaggc attactggca gaaggtgtga tggtatcggg aagcgggcaa
     4861 atcgacttgc agcgttatcg ctggaactac taaccctctc cgggcggaga gggttaactg
     4921 cgcttaatat tgcgtaggag ctggaactgc cgaagacgga ttcacctgag ttggtgaagt
     4981 cgaaggtacg gtagtcgttg cgccaccgct ggcctgaacc ggcacggcgg tttgctgcac
     5041 cggcaccaat gtcaggtcgg ctttagttcc gccctggttg atcaccggct gaacggtatc
     5101 ggtaataaat accagtttgt cattcacggt aatcgccgca ctcaacagaa tacgcgcatt
     5161 cggctgaaca tctgccgggt taaatggcag aacaaagctg aatggtgact gtttgccttc
     5221 ggtacgcacc gctttctgcg ccagtacttt tgacggtgca tcggctaacg acgcgtcaga
     5281 aagtgtcacg gtcagcacag catcaggcgg cagtgcgact ttctgacgga tccagacggt
     5341 accggagaca ttcggttgct ggatagcagg ttgttgtgtt gctgaaatag acgtatttgc
     5401 agccggtgct ggcgtctgaa tatccgcgct tttatctgcg caagccgcca acgcaatcgc
     5461 aaccgctaaa ccactggcca tgtgcacgag tttcattcac ttctccttat tatcaatgca
     5521 ccagcgggct aactttcctc gccggaagag tggttaacaa aatagtaacg tcaacaagtg
     5581 tggcacacat cacgcatttc tgcctgtaat tagcccgtaa ttcagaccat tgcacccatc
     5641 ggaccagtgg caaatttgcg ttatactcaa ctcactttgg cttgctgcgg cagctttgtt
     5701 actggagagt tatatgagtc aggcgctaaa aaatttactg acattgttaa atctggaaaa
     5761 aattgaggaa ggactctttc gcggccagag tgaagattta ggtttacgcc aggtgtttgg
     5821 cggccaggtc gtgggtcagg ccttgtatgc tgcaaaagag accgtccctg aagagcggct
     5881 ggtacattcg tttcatagct actttcttcg ccctggcgat agtaagaagc cgattattta
     5941 tgatgtcgaa acgctgcgtg acggtaacag cttcagcgcc cgccgggttg ctgctattca
     6001 aaacggcaaa ccgatttttt atatgactgc ctctttccag gcaccagaag cgggtttcga
     6061 acatcaaaaa acaatgccgt ccgcgccagc gcctgatggc ctgccttcgg aaacgcaaat
     6121 cgcccaatcg ctggcgcacc tgctgccgcc agtgctgaaa gataaattca tctgcgatcg
     6181 tccgcttgaa gtccgtccgg tggagtttca taacccactg aaagggcacg tcgcagaacc
     6241 gcatcgtcag gtgtggatcc gcgcaaatgg tagcgtgccg gatgatctgc gcgttcatca
     6301 gtatctgctc ggttacgctt ctgatcttaa cttcctgccg gtagctctac agccgcacgg
     6361 catcggtttt ctcgaaccag gaattcagat agcgaccatc gaccattcca tgtggttcca
     6421 tcgcccgttt aatttgaatg aatggctgct gtatagcgtg gagagcacct cggcgtccag
     6481 cgcacgcggc tttgtgcgcg gtgagtttta tacccaggac ggcgtgctgg ttgcctcgac
     6541 cgttcaggaa ggagtaatgc gtaatcacaa ttaaaaaaat agccggaggt gaaaaccgtc
     6601 cggctgtttt ttgcagtgct tgttacgcgt tataggcatt ttcgccgtgg ctgttgacgt
     6661 caagcccttc tcgctcctgt tcttccggca cacgcaggcc aaccgtcaga tccgccagtt
     6721 tgtagccgat aaatgccacc acaccggacc agacgatcgt aatggcgatg ctttccagct
     6781 gtaccagcaa ctgatggccc atcgtcacac cttcagcgaa gcccacgccg cccagcgaac
     6841 tggcggcaaa aatcccggtc atgatacagc cgacaatgcc acaaacgccg tgcacaccga
     6901 agacatcgca gggatcatcc acccgcagca agcgtttgag catggtaacg ccccacaagc
     6961 ccgccagacc agctaccacg ccgataatca acgcgccgcc aacaccaatg tagccacacg
     7021 ctggcgtcac gccgaccaga ccggcaatcg cgccagaaca cgctcccagc agtgaaggct
     7081 ttccgcgcag cgcccattca ccgaagatcc agccaagaat tgctgccgcc gttgcgacca
     7141 cagtattcac aaatgccagt gccgcgattt cattcgccgt gcccgctgac ccggcgttaa
     7201 agccaaacca accgatatag agaatggcag tcccggtgaa gaccatcggc aggttgtgcg
     7261 gtttaaacgc ctctttaccg aagcccacgc gtttgcctat cagatatgcg cccaccagac
     7321 cggcgattgc ggcgttaatg tgcaccaccg tgccacccgc gaaatccagc gcaccgtgag
     7381 aagccagcaa accaccgccc cagaccatat gcgcaatcgg aatgtaggag agcgtcagcc
     7441 ataccaccac gaaaatcaac acagctgaga agcggattcg ttccgccagc gccccaacta
     7501 tcaagcctac ggtaatgcag gcaaacgatc cctgaaacgc tacgtggata tactgataaa
     7561 tgctgcccat caccgccgtc agttcgatgt ttttcagcat caaccagtta atgttgccga
     7621 agaagttgtt tccctcacca aacgccagcg agtaaccgta aaccacccag agaatacaga
     7681 ccagtgcaaa cgtcaccgtc acctgcgtca gcatcgacag cacgtttttg ccgcgaatca
     7741 acccaccgta aaacagggca atccccggaa tagtcataaa cagcaccagc gcagtacaaa
     7801 tcatcataaa cgcattgtcg gctttatcgg ccaccgcagg tgcagccatt accagtcccg
     7861 gcagcatcgc cagtgaagca agcccagttt ttatcgtcgc tatcttcatt tttcgttcct
     7921 gttgctgtgt gccagagatt acagcgccga ttcgtcggct tcgccggtac gaatacgaat
     7981 gacgcgttgc aattcagcga cgaagatttt gccgtcgcca atttttccgg tgtaagccgc
     8041 tttgctgacg atatcgatca cttcatcgag ttggtcatca gcaatcgcca catcaatttt
     8101 tacttttggc aggaaattga cgctgtattc cgccccccgg tacagctcgg catgtccttt
     8161 ctgacgcccg aaacctttca cttcggtgac ggtcaggccc tgaataccaa tggaagataa
     8221 cgcttcacga acgtcttcca gcttgaatgg tttgattatc acggtcacca gcttcataga
     8281 tcccctccgg tcagaattcg gtaatggttc tgctacacga agaaggtatt gcaagcggtg
     8341 tgccagaaat gaaaaacgag aaggagagca ggaagttaag aagattgaaa acaaaaacgc
     8401 actatgacag tgcacagtgc gttacatttt tgcaccaacg atgaaatatt gcgctattca
     8461 ggcgctcaat gattcctctt cacgcacgct ggctgccagc tcttcgcccg caagttgcag
     8521 ttgatacatc tgccagtagc gtccctgggc cgccagcagt tgctggtgag taccctgctc
     8581 cacggcttgc ccacgatgaa gcaccagaat ggtgtcggca tcaacaatgg tcgataagcg
     8641 gtgagcaatc accaccagcg tggtatgttc acgcaccgcc gccagagcat gttgaatcgc
     8701 ctgttcagta ccggagtcaa tgctggcggt tgcctcatca aggatcagga tttgcggcgt
     8761 ctccaccaac acgcgtgcca gtgccagcag ttgcttttgc ccgactgaga gattattccc
     8821 ctgctcgcca agcggcgtgt aaataccgtc gctcatgcta cgcgccagct ccgccagttg
     8881 cacggtttcc agcgcctgcc agacgcgttc ttcggagata tcacgcccca gcgtcacgtt
     8941 ggcgaggaag gtatccgcca gcaccaccgg atcttgctgc accattgcca cgccctggcg
     9001 cagcgcgctg tgacttagcg aacttaatgg acgaccatca aggcgaatct caccttccgt
     9061 tagcgggtaa tagcccatca ataaactggc gagggtgctt ttgccactgc cggtatgccc
     9121 gaccagtgcc acaaagttac gcgaaggcac agagagatta atgttcttta gcaccagatt
     9181 gtcatcgcga taagcaaatg acacgttatc gacttcgatg gtgccgctct gtaacgggcg
     9241 atcatcattg ccatattgct ggcgcggtcc gtccatcagt tcaaacacac gctcaccagc
     9301 aactacagcc tgttgcagca tcgcctgctg cgtggtcagt tcgattaatg gttcgttaag
     9361 tcgcccaaga tagctgataa atgcatacaa cacgcccact tcaatggtgc cgctggcgga
     9421 gaagccaaac agcatcaaca acccacaaag aatgagcgat gaaaacagac tcagcagcgg
     9481 acgcagcaga aaaccgtcga ggcgcagggt ttgcatcctc gccatatagt gtgaacgact
     9541 ggcctcaccc atacgttcgc caaatcgcga ctgctgacgg aactgctgga taacgctcat
     9601 gccattgatg atttcgttaa agccgtcgtt gatatctgcc agataagcgc gtacacggcg
     9661 gacgatcggc gtgctgtaac gctgatatat caccattacc accagcacca ccgggaaaat
     9721 cattatcgcc accagcgcca ttcgccagtc gaggctgaac atcgacacca gcatcgcgcc
     9781 caccagtgcg gcgctacgca ggacagttgc cactacggta acgtagaggt cgcggatcac
     9841 ttcagtgtca ttagtgacgc gggaaatcac ctgcccgacg ggttgggtat caaactcgct
     9901 taatggctgg cgtaacgcag catccatcac gtcggtacgc aactgttgca ctacgcctac
     9961 tgccgcccga ttaaacagca gcgactgcgc gtaatgtagc ccggcggcaa acagttgcag
    10021 cccaacatac gccgcagcca gccctgcaac cactttcaac ggcaggttat ttttcgctac
    10081 catattgtcg ataaaatagc tgataagcag cggcccactg acttctgccg ctgccgcaac
    10141 ccacatcatc aggaccgcaa tccccagcgg tttacgccac ggcgaaccgt acgctaacag
    10201 acgcttgaga gtcggccaca gttggctaaa actacgcatc aacggcctcc tcgcgaattt
    10261 ccggagcgtc gtcgagcgcc gcctccagtt gttgatagcg atacatatcg cgataccagc
    10321 cgctttgttg cgccagctca tcatgattgc cacgctgagc gatatgtccg tgctgcatca
    10381 caataatttc actggcttcc gtcagtgccg aaaggcgatg ggcactgatg attaccgttc
    10441 ttccctgccc ccattgacgc aggttatgca ggatctggtg ctctgtgcgt ccgtctactg
    10501 ccgaaagcgc atcatcaagg atgaggattt ccgcgttgac taataacgca cgagcaatgg
    10561 agatacgctg tttttgccca ccggaaagca tcacgccgcg ctcgcccact tctgtatcgt
    10621 aaccttgcgg taaacgcaaa atatcgtcat gcacgctggc taaccgcgcg acatgctcaa
    10681 tctcttgctg ggtggcattc gggcaaccca acgcgatgtt gttcgctacg gtgtcggaaa
    10741 aaaggaacgg tgtctggcta actaccgcca ggcggctacg ccagctatcg agttgtaact
    10801 tcgtcagagg aatatcatga aagcggatat ccccctcgct gacgtcgaaa tgacgctgaa
    10861 tgagcgacaa cagggtactt ttgccggaac cagtcggccc gcaaataccc agcatctgac
    10921 cgggtttcag ggcgaaattg acgttttcca gcgcaggatg gtcagtctgc ggatacgtga
    10981 actggcgaat atttacatcc agttcgccac gaccttccgg cacgggttcg ctaccatcat
    11041 tcaccaccgg cgcttccgcc agcatcgcgc gaatgcggct gtacgcagca ctaccacgtt
    11101 ccactatgtt aaacatccat gccagcgcca gcattggcca aatcatcaga cctaaataca
    11161 tcataaaact ggtgagctgg cccagcgtta aactaccctg caccaccatc caactaccgc
    11221 cgccaatcgc cagcaagttc gccgtgccga tggcgatgta aatggtcgga tcaaaacggg
    11281 catcaatacg tgccacacgc atgtttttct tgccggtatc ttcggcatcc tcggcaaata
    11341 acgccgactg gcgatcttcc agaccaaaag ctttgatcat gcggatactg gtgaggcttt
    11401 cctgggtgcg gtcattaaga ctagaaaacg ccgcctgtgc cagcttaaag cgttcatgca
    11461 aggcatcgcc gttgcgcttg atcatgatcg ccatcactgg catcggcaac agggcaaata
    11521 aggtcaactg ccagctaatt tgcgtagaca tcataatcaa cacagcgcag cccatcacca
    11581 gtgaatccac cagcgtcagc accccttctc cggcggcaaa cacgacgcga tcgacgtcat
    11641 tggtcgcacg agccatgagg tcacccgtgc gatgacgcag gtaaaactca ggatgctgcc
    11701 ggcttagctg acggtaataa tcttcacgca gttcaacagc cagttgataa gacgcaccaa
    11761 acagcaatac gcgccagacg taacgcagga gataaaccac aacggcaatc agcaccatgg
    11821 tggcgatcca catcaggatc tgcccggtag taaagtgttg ttctgtcacg ccatcgacaa
    11881 caataccaac cacttttggc ggaaccagtt gcagcatcgc gataatgaca agcaaggcga
    11941 cagccccgag atagcgacgc cattcccgac ggaaatacca gcttaattga gcaaataatc
    12001 gcacgcgttg tgtcctgacc tgattctgga tatttattcg atgggtaaag aagtggtgta
    12061 tttaatctgt tccatcgcga agctggaagt gacgtccgac agccccggca cgctgtttac
    12121 caggcgctta taaaactcgt cgtagcgttt catgtcggca acctggacgc gcatcagata
    12181 atcgtattca ccagccatgc gccagaaccc cagcacttct ggcatttcgg taaccaccgt
    12241 gacaaagcgg caataccatt cgctgctgtg atgttgcgtt tttatcagca caaaggcggt
    12301 caggccgagg cctatttttt ccggatccag cagggcgact ttgccgataa gaataccgtc
    12361 gtcctccagc cgtttcagac gcttccagca gggggtagtt gtcagattaa cggcttcagc
    12421 cagtgcctgc aaagagaggg tgcaatcctg ctgtagtaag gccagcagct tacggtcaat
    12481 tttatctaac atagcccttc cacggagaat ttttttctct tttaattcta ttttaaaggt
    12541 gaaatcgcga caatttattc tgtgcggttt aaacaccacg gcacaaaatg ac
//
LOCUS       DAOTRK010000131        12587 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.132, whole genome shotgun sequence.
ACCESSION   DAOTRK010000131 DAOTRK010000000
VERSION     DAOTRK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.132"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1507
                     /gene="caiT"
                     /locus_tag="QIE41_003662"
     CDS             <1..1507
                     /gene="caiT"
                     /locus_tag="QIE41_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDS3532501.1"
                     /translation="EKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYV
                     TNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSAAVL
                     FWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYFFFV
                     RKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQW
                     LFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGA
                     SFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIFLAR
                     ISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVARAII
                     ETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRIGWS
                     ILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1538..2680
                     /gene="caiA"
                     /locus_tag="QIE41_003663"
     CDS             1538..2680
                     /gene="caiA"
                     /locus_tag="QIE41_003663"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDS3532502.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLLPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2809..4026
                     /gene="caiB"
                     /locus_tag="QIE41_003664"
     CDS             2809..4026
                     /gene="caiB"
                     /locus_tag="QIE41_003664"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDS3532503.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTSEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGKIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4100..5653
                     /gene="caiC"
                     /locus_tag="QIE41_003665"
     CDS             4100..5653
                     /gene="caiC"
                     /locus_tag="QIE41_003665"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDS3532504.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLIDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIAAHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5762..6547
                     /gene="caiD"
                     /locus_tag="QIE41_003666"
     CDS             5762..6547
                     /gene="caiD"
                     /locus_tag="QIE41_003666"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDS3532505.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6553..7143
                     /gene="caiE"
                     /locus_tag="QIE41_003667"
     CDS             6553..7143
                     /gene="caiE"
                     /locus_tag="QIE41_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDS3532506.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7262..7657)
                     /gene="caiF"
                     /locus_tag="QIE41_003668"
     CDS             complement(7262..7657)
                     /gene="caiF"
                     /locus_tag="QIE41_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDS3532507.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7918..11136)
                     /gene="carB"
                     /locus_tag="QIE41_003669"
     CDS             complement(7918..11136)
                     /gene="carB"
                     /locus_tag="QIE41_003669"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDS3532508.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECESNPSDREKIMVLGG
                     GPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAVE
                     RLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQTA
                     VSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPAY
                     TLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKAT
                     GVPLAKVAARVMAGKTLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRSTG
                     EVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELDA
                     THGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIRR
                     SALQYKVHYDTTLNGGFATAMALNADATDKVISVQEMHAQIK"
     gene            complement(11154..12302)
                     /gene="carA"
                     /locus_tag="QIE41_003670"
     CDS             complement(11154..12302)
                     /gene="carA"
                     /locus_tag="QIE41_003670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDS3532509.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAETYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2918 a         3296 c         3359 g         3014 t
ORIGIN      
        1 tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg
       61 catactttgt tggcttacag tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt
      121 attcagttac gtcaccaatg tatggggatg ggcatttgaa tggtatatgg tagtgatgct
      181 tttcggttgg ttctggctgg tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc
      241 acctgaattt agtaccgcca gttggatctt tatgatgttc gcctcctgta cgtctgctgc
      301 cgtactgttc tggggatcga ttgagatcta ctactacatc tccaccccgc cgtttggctt
      361 agaaccgaac tcgacagggg cgaaagagtt ggggctggct tacagcttgt tccactgggg
      421 acctctgccg tgggccactt acagcttcct ttcagtcgcc ttcgcttact tcttctttgt
      481 ccgcaaaatg gaagtgattc gccccagctc gacactagtg ccgctggtag gtgaaaaaca
      541 cgccaaaggg ttgttcggca ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc
      601 gatgggcacc agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt
      661 tggcattccg cataccctgc aactggacgc tatcatcatt acctgctgga ttatcctcaa
      721 cgccatttgc gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag
      781 ttacctgagc ttcctgatgc tgggttgggt gttcattgtc agcggtgcca gcttcatcat
      841 gaactacttc accgattcgg tggggatgtt gctgatgtat ctgccgcgca tgttgttcta
      901 taccgatccc atcgctaaag gcggcttccc gcagggctgg accgtgttct actgggcatg
      961 gtgggtgatt tacgctatcc agatgagtat cttcctcgcc cgcatctccc gtggtcgtac
     1021 cgtgcgtgaa ctgtgcttcg gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg
     1081 gactgtactc ggtagtaaca ctctgctgtt gatggataaa aacatcatca acattccaaa
     1141 tctgatcgaa cagtacggtg tggcgcgcgc catcattgaa acctgggccg ctctgccact
     1201 cagcaccgcc accatgtggg gcttcttcat cctctgcttt attgccaccg ttacgctggt
     1261 taacgcctgc tcttataccc tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga
     1321 accaccgctg ctggtgcgta tcggctggtc aattctggtt ggcattatcg gtattgttct
     1381 gctggcgctc ggcggcctga aaccgattca aaccgccatt atcgccggag gatgcccgct
     1441 gttcttcgtc aacattatgg tgacgctctc ctttattaaa gacgcgaaac agaactggaa
     1501 agattaatta acccccaaaa tatcaagagg ttgaaagatg gattttaatt taaatgatga
     1561 gcaggaactg tttgtcgccg gtatccgcga acttatggcc agcgaaaact gggaggccta
     1621 ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag cactggcgga
     1681 tatgggtatc gacagtctgc tgctccctga agagcacggt ggtctggacg cggggtttgt
     1741 tactctcgcc gccgtgtgga tggagctggg acgtctgggg gcaccaacct atgtactgta
     1801 ccagttgccg ggcgggttca acaccttcct gcgcgaaggc acacaagagc agatcgacaa
     1861 aattatggct ttccgcggca ccggtaagca gatgtggaac tcagcgatta ccgaaccggg
     1921 cgcgggctcc gacgtgggta gcctgaaaac gacttatacc cgtagaaatg gtaagattta
     1981 tcttaatggt agtaagtgtt ttattaccag cagcgcctac accccgtaca tcgtggtgat
     2041 ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc gaatggtttg ttgatatgag
     2101 caaaccgggc atcaaagtga ccaaacttga aaagctcggt ctgcgtatgg atagctgctg
     2161 tgaaatcacc tttgacgatg tggaactgga cgagaaagac atgttcggtc gggaaggtaa
     2221 cggctttaac cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag ccctgaccaa
     2281 ctacggtacg gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca
     2341 gtttggcgag gctattggtc gtttccagtt gattcaggaa aaattcgccc acatggcgat
     2401 caaattaaac tccatgaaaa acatgctgta tgaagcagcg tggaaagcag acaacggcac
     2461 catcacctct ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg catttgaagt
     2521 ggtggatagc gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag
     2581 ccgcttctgg cgtgatctgc gtgtagaccg cgtctccggg ggatctgacg aaatgcagat
     2641 cctgacgctg ggtcgcgcgg tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc
     2701 gacgctgacg cgtcttatca gacctacggg agcacgaatg taggccggat aaagcgttta
     2761 cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat ggatcatcta
     2821 cccatgccga aattcgggcc gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc
     2881 gccgggccgt tcgccgggca aatgttcgca gaatggggcg cggaagttat ttggatcgag
     2941 aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcagct ctcccgccgc
     3001 aatttgcacg cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc gtttctgaaa
     3061 ttaatggaaa ccaccgatat cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt
     3121 ggcattaccg atgaagtcct gtggcagcac aacccgaaac tggttatcgc tcacctgtcc
     3181 ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa caccatcgcc
     3241 caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat gcctgccttc
     3301 ccgtataccg ccgattactt ttctggtctg accgccacca cggcggcgct ggcagcactg
     3361 cataaagtgc gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg
     3421 ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat gtgcccgcgc
     3481 atgaccaaag gtaaagatcc ctactacgcc ggttgcggtc tatacaaatg cgccgacggc
     3541 tacatcgtga tggaactggt gggcattact caaattgaag agtgctttaa agatattggc
     3601 ctcgcacatc tgctcggtac gtcggaaatc ccggaaggca ctcagcttat ccaccgtatc
     3661 gaatgccctt acggcccact ggtagaagag aaactcgatg cctggctggc tgcacatacc
     3721 atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc
     3781 gtaccggaac tggaaagcaa tccacagtat gtggctcgcg aatcaatcac tcagtggcaa
     3841 acgatggatg gtcgcacctg caaggggccg aatatcatgc cgaaattcaa aaataacccc
     3901 ggaaaaatct ggcgcggaat gccctcacat ggcatggaca cggctgccat tttgaaaaat
     3961 atcggctaca gcgaaaacga cattcaggag ttggtcagca aaggtctggc caaagttgag
     4021 gactaaacgc gtgacgtcag tcggcttgcc tgactagcgc ccgcccaata acaaacgaac
     4081 aacaatggat agaggtgcaa tggatatcat tggcggacaa catctacgtc aaatgtggga
     4141 cgatctggcg gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca gcagcggagt
     4201 cgttaaccgg tatagttatc ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt
     4261 ttatacgctg gggattcgca aaggcgacaa ggttgctcta catctcgaca actgcccgga
     4321 atttatcttt tgctggttcg ggctggcaaa aattggcgcg attatggtgc cgattaacgc
     4381 ccgcctgtta cgcgaggaaa gcgcgtggat cctgcaaaat agccaggcgt gcctgctggt
     4441 gaccagtgcg caattttatc ctatgtatca acagattcag caggaagatg cctctcagtt
     4501 gcggcacatt tgcctgatag atatggcact tcccgctgat gatggcgtga gttcgtttac
     4561 tcaactgaaa aatcaacaac ctgccacctt gtgctatgca ccgccgctat cgactgacga
     4621 tacggcggaa attctcttca cctccggcac cacctcccga ccgaaaggtg tggtgattac
     4681 ccattacaac ctgcgcttcg ctggatatta ctccgcctgg caatgtgcac tgcgtgacga
     4741 tgacgtctac ctgacggtaa tgcctgcgtt tcatattgac tgccagtgta ctgcggcgat
     4801 ggcggcgttt tctgccgggg ccacctttgt gctggtcgag aaatacagcg cccgcgcctt
     4861 ctggggacag gtgcagaagt accgcgccac cattaccgaa tgtattccga tgatgattcg
     4921 tacgttgatg gtccagccgc cttcagccaa cgatcggcaa cactgcctgc gggaagtgat
     4981 gttttatctc aacctgtcgg agcaggaaaa agacgcgttt tgtgaacgct tcagcgttcg
     5041 cttgctgacg tcttatggga tgacggaaac cattgtgggc attatcggcg atcgccctgg
     5101 cgataaacga cgctggccgt cgattggtcg ggcgggcttt tgctacgaag cggagatccg
     5161 cgacgatcac aatcgtcccc tcccggctgg tgagctcggt gaaatctgta ttaaaggcgt
     5221 accagggaaa accatcttca aagagtattt tctcaacccg aaagccactg cgaaagtgct
     5281 ggaagccgat ggctggctac ataccggcga taccggatac cgcgacgaag agggcttttt
     5341 ttatttcgtc gatcgccgct gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt
     5401 ggagctggaa aatattatcg ccgcgcaccc gaaaattcag gacatcgtgg ttgtgggtat
     5461 taaagattcg atacgcgatg aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac
     5521 attgagcgaa gaggaatttt tccgcttctg cgaacaaaat atggcgaaat ttaaagtgcc
     5581 ctcttatctg gagatcagaa aagatctgcc acgtaattgc tcggggaaaa taattagaaa
     5641 gaatcttaaa taaatgaaac ggcagggaac taccctgcca gctaataatc acgctctaaa
     5701 acaatatgca ttttttgcag agatgttgtc gtccctgaaa aagcaaaaat ggagaaaagg
     5761 aatgagtgaa tcattacatc tgacccgcaa tggatcaatt ctggaaatta cccttgatcg
     5821 cccaaaagcg aatgctattg atgcaaaaac cagctttgaa atgggcgaag tatttctaaa
     5881 tttccgtgac gatccgcaat tacgtgtcgc cattattacc ggtgccggag agaaattctt
     5941 ttccgcgggc tgggatttaa aagcggcagc agaaggcgaa gcaccggatg ctgactttgg
     6001 cccgggtggt tttgcgggat taaccgaaat tttcaatctc gacaaaccag ttatcgccgc
     6061 tgtgaacggc tatgcctttg gcggcggctt tgaactggca ctggcggcag attttattgt
     6121 ctgtgccgat aacgccagct tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag
     6181 cggcggcgtg ctgcgtctgc cgaagatcct gccgcctgcc atcgtcaatg aaatggtgat
     6241 gaccggcaga cgaatgggcg cagaagaggc gctgcgttgg ggggtagtca accgcgtggt
     6301 tagccaggcg gaactgatgg ataacgcccg cgaactggcg cagcagctgg ttaacagcgc
     6361 tccgctggcg attgcggcgc tgaaagagat ctaccgcacc accagcgaaa tgccggtaga
     6421 agaagcgtat cgctatattc gcagcggcgt gttgaaacac tatccgtcgg ttctgcattc
     6481 ggaagatgcc attgaagggc cgctggcgtt tgccgagaag cgcgatccag tgtggaaagg
     6541 acgttaacaa ccgtgagcta ttacgccttt gaggggttaa ttccggtggt tcacccgacg
     6601 gcgtttgtcc atcccagtgc agtcttgatt ggcgatgtga ttgtgggagc cggtgtctac
     6661 atcggcccac tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc
     6721 aatattcagg atggctgcat tatgcatggc tactgcgaca ctgacaccat cgttggggaa
     6781 aacggtcata tcgggcacgg agcgatcctg catggttgtg tgattggtcg cgatgcactg
     6841 gtcgggatga acagcgtgat tatggatggc gcggtcattg gcgaagagag cattgttgcc
     6901 gctatgagct ttgtcaaagc gggctttcgc ggcgagaaac gccagttgtt gatgggtacg
     6961 ccagcccgcg ctgtacgcag cgtgagtgac gacgagttac actggaaacg gttgaatacc
     7021 aaagagtatc aggatcttgt cgggcgctgt catgcatcgt tacatgaaac gcagccactg
     7081 aggcaaatgg aggaaaatcg cccccgtttg caggggacga cggatgtgac gccgaaacgg
     7141 tgatcttgcc ggatgcggcg taaacgcctt atccgacctg gctggaaagc aaccgtaagc
     7201 cggataagat gcgccagcat cgcatccggc gatcaggtcg cacggtatca tcgaaatgac
     7261 attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg ttaagttccg
     7321 tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta tcccggctgt
     7381 tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cactttaacc agtcgctgac
     7441 actgacatcc ccgcccttcc agcttattag ggatcatctt aacttcgcag cttatttctg
     7501 tgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca atatcgaaat
     7561 gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat tcggcgatta
     7621 acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt tcacaaatta
     7681 aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa attgtggata
     7741 tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct tatgtcatca
     7801 aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaacggtcc tcatcagaga
     7861 accgtattag ttattcgatc aaattagcgg atgaaaaata tccgccatga cacgctatta
     7921 tttgatctgc gcgtgcattt cctgcaccga aattacttta tcggtcgcat cagcattcag
     7981 cgccatcgca gtagcgaaac caccgttcag ggtggtgtca taatgcactt tatattgcag
     8041 cgcactgcga cgaatcacgc gggagtcttc aatcgcgcga cgacctgagg tggtgttgat
     8101 gatgtaggta tattcgccat tcttgatgcg gtcctgaatg tgcggacggc cttcatgcac
     8161 cttgtttacc agacgcggat tgatacccgc ttcgcccagc acaatcgccg tgccgtgggt
     8221 cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca cgcgttcttt
     8281 gtcgccttcg cgcacggaaa gcagcgcacg accgtgtttc ttcatggtgg agttgctgcc
     8341 cagctgcgct ttggcgaacg cttcagcgaa ggtgcggccc acgcccatga cttccccggt
     8401 agagcgcatt tctggcccta acagcgggtc aacgccgggg aatttattga acggcagcac
     8461 cacttctttc accgagtagt acggcgggat aacttctttg gttacgccct gctcggccag
     8521 cgttttgcct gccatcacgc gcgccgccac tttcgccagc ggtacgccgg tggctttgga
     8581 gacgaacgga acggtacgcg ccgcacgcgg gttaacttca atcaggtaga cttcgttgtt
     8641 tttaaccgca aactgcacgt tcatcaggcc gcgcacctgc aattcgaagg ccagtttctg
     8701 cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtaggctg gcagagaaca
     8761 tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc tccatgatgc cgccaatcag
     8821 caccatttca ccgtcgcaga tggcatccac gtcaacttct actgcgtcat caaggaaatg
     8881 atccagcaac actggcgcat cgttagacac gctaaccgcc gtctggaagt agcgacgcag
     8941 gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag acggacgtac
     9001 caccagcggg tagccaatct ctttcgcttt ctcaaccgcc atttcgatgg tggtaacggt
     9061 ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca tgctggaagc gttcacggtc
     9121 ttccgcacgg tcgatggcat ccgggctggt accgataacc ggtacgccag cagcttccag
     9181 cgcgcgcgcc agtttcagcg gggtctgacc gccgtactgg acgataacgc ctttcggctt
     9241 ctcaatacgc acaatttcca gcacgtcttc cagagttacc ggctcgaagt agaggcggtc
     9301 ggaagtgtcg taatcagtgg agacggtttc cgggttacag ttaaccataa tggtttcgta
     9361 accgtcttcg cgcagcgcca gtgaggcgtg tacgcaacag tagtcgaatt cgataccctg
     9421 accgatacgg ttcgggccgc cgccgaggac catgattttt tcacggtcag atgggttgga
     9481 ttcgcactct tcttcataag tggagtacat ataagcggtg tcggtagcga actctgccgc
     9541 acaggtatcc acgcgcttgt aaaccgggtg caggtcatac tggtcacgca gcttacggat
     9601 ttctgcttca cgcacgcccg cgagttttgc cagacgcgcg tcggcaaagc ctttgcgttt
     9661 caactggcgc aggaagtcag cgttcaggcc agtgatgccc acttccgcca ctttctcttc
     9721 cagacgcacc agctcttcaa tctgtaccag gaaccagcgg tcaatgttgg tcaggttgaa
     9781 gacgccgtct acggacaggc ccgcgcggaa agcatcggcg atataccaaa tacgctctgc
     9841 gccagcgtct ttcagctcgc ggcggatttt agtcagcgct tccgggtcgt cgagactcac
     9901 tttcggatcg aagccggtcg cgcccacttc caggccgcgc agcgcttttt gcagggattc
     9961 ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat ttcatctgag tggtcagacg
    10021 gtcgttcgcc ccggcgaatt tctcgaagtt gaagcgagga attttggtga ccacgtagtc
    10081 gatggacggc tcgaaggagg ccggagtacg cccgccggtg atgtcgttca tcagctcgtc
    10141 gagggtgtaa cccaccgcca gtttcgccgc cactttggca atcgggaaac cagtggcttt
    10201 cgacgccagc gccgaagaac gcgacacgcg cgggttcatt tcgataacaa tcaggcgacc
    10261 gtttttcggg ttcaccgcaa actggacgtt ggaaccaccg gtttcaacgc cgatttcacg
    10321 cagcaccgcc atcgaggcgt tacgcatgat ttgatattct ttgtcggtca gcgtttgggc
    10381 tggtgcgaca gtgatggagt caccggtgtg gatgcccatt gcatcgaagt tttcgataga
    10441 gcagacgatg atgcagttgt cgtttttatc acgcaccact tccatctcgt actctttcca
    10501 gccgatcagc gactcatcaa tcagcagttc tttggtcgga gagagatcca gaccgcgggc
    10561 gcaaatttct tcaaactctt cgcggttata agcgataccg ccaccgctac cacccatggt
    10621 aaaggatggg cgaataatgc acgggaagcc cacatcagcg gcaaccgcca gcgcttcttc
    10681 catcgtgtgt gcgataccgg aacgcgcggt ttccagacca attttcttca tcgctacgtc
    10741 gaaacgacgg cggtcttctg ctttatcaat cgcatcggca gtggcaccaa tcatggtgac
    10801 gccgaactct tccaacacgc cctgacgttc cagctccagc gcacagttca gcgccgtctg
    10861 accgcccatc gttggcagca ccgcgtccgg gcgctctttt tcaataatct tgcgtaccac
    10921 ttcccagtga atcggctcga tgtaggtcgc atcagccatt tccgggtcgg tcatgatggt
    10981 cgccgggttg gagttcacca gaatgacgcg gtaaccctct tcacgcaggg ctttacacgc
    11041 ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata acaatcgggc ccgcacccag
    11101 aatcaggata ctttttatat ctgtacgttt tggcatggct cttttactcc tgattactta
    11161 gcggttttac ggtactgctc aattaactcg ataaagtggt cgaacaacgg cgctgcgtcg
    11221 tgtggcccag ggctggcttc agggtgaccc tggaagctga acgccggttt atcggtgcga
    11281 tgaatgccct gtaacgtacc gtcgaacagg gatttatgcg tgacacgcag gtttgcaggt
    11341 aatgttgctt catccaccgc aaaaccgtgg ttctgggcgg tgatcatcac cacgtttttc
    11401 tccacatctt taaccggatg gttgccgccg tggtgaccaa atttcatttt gacagtcttc
    11461 gcaccgctcg ccagcgccag cagctgatga ccgaggcaga tgccgaagac cggaatatcg
    11521 gtttcgagga atttctggat ggcggtaatg gcgtaatcgc acggggccgg gtcgccagga
    11581 ccgttggaga ggaagatgcc gtctggattc attttcaaca catcttccgc agaagtttgc
    11641 gccggaacga tggtcaggcg gcagcctcta tccaccaaca tgcgcaggat gttgcgcttg
    11701 gcaccaaaat cataagccac aacatggaac ggcagctcgt cttctttctt cgcttctggc
    11761 aggccgccgg tcagcgtcca gctcccttgt gtccagctat acgtttctgc ggtggtcact
    11821 tcttttgcca gatccatgcc gttcagacct gggaacgcgc gggctttttc taacgccagc
    11881 gccgcatccg ggttatcgcc cgcgataatg cagccattct gtgcgccttt ctcgcgcagt
    11941 aaacgcgtca gcttacgggt atcgatatcg gcaatcgcca cgatgttatg gcgcttcagg
    12001 taagaagaga ggtcttcggt attacggaag ttgctggcaa tcagcggcag gtcgcgaatc
    12061 accagacctt gtgcatgtac ctgagaagat tcttcatcgg cgtcattagt gccgacattg
    12121 ccaatatggg gataagtaag agtaacgatt tgacgagaat aggaaggatc agtgaggatt
    12181 tcttgataac cggtcattga agtattgaaa acgacttccc caaccgccga acctgttgcc
    12241 cctatggccc gaccgtgaaa ctgggttccg tcttccagaa ccaatagcgc tgacttaatc
    12301 aaaacaccct ccagagaata ttcactcact ttatttgcat attaattcac aatgatgacg
    12361 taaatcaatg caaatctgct taccgacgaa tttctggcaa acggcggcat tctggagata
    12421 tgggagctaa aagtcaactt aatggcggga ttttttatat tattttgtgg ctcatgcatg
    12481 ttttacaggc attaagcgac aaaaagatca tataacatgt tctggaaaac gcttgcgcag
    12541 caagaagatt gcatgaaatc aaaatgagca gaaaaaagtg gaccaaa
//
LOCUS       DAOTRK010000132        12453 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.133, whole genome shotgun sequence.
ACCESSION   DAOTRK010000132 DAOTRK010000000
VERSION     DAOTRK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.133"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            83..451
                     /gene="cbtA"
                     /locus_tag="QIE41_003671"
     CDS             83..451
                     /gene="cbtA"
                     /locus_tag="QIE41_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDS3532510.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            540..917
                     /locus_tag="QIE41_003672"
     CDS             540..917
                     /locus_tag="QIE41_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDS3532511.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPGAKQ"
     gene            914..1402
                     /locus_tag="QIE41_003673"
     CDS             914..1402
                     /locus_tag="QIE41_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532512.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSATLTGIRCSTAHITETDNAWLFSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIQRYGVSLIHLDASAECLPDQPTF
                     DW"
     gene            1414..1611
                     /locus_tag="QIE41_003674"
     CDS             1414..1611
                     /locus_tag="QIE41_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532513.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDRTDSAALLPC
                     IEQARKDVRALRHLQLLHQNR"
     gene            1696..2541
                     /locus_tag="QIE41_003675"
     CDS             1696..2541
                     /locus_tag="QIE41_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDS3532514.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIQHVVTVRNAALQQTETLIRQ
                     LAEISVLTAGIGGKTALDWAMKQNFRCGCWLMEKPEAAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKNNSPCKFGSKIIVNNLVRWDRWGFHLNNGPQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDEHIRSVQGKECYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     MLPSQ"
     gene            2722..2904
                     /locus_tag="QIE41_003676"
     CDS             2722..2904
                     /locus_tag="QIE41_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532515.1"
                     /translation="MPCNTVNPDIFYCSIFNRLQEYLVKGFVMDDERLKNPPVGSSVL
                     NASAICAPACAVFICR"
     gene            3332..4516
                     /locus_tag="QIE41_003677"
     CDS             3332..4516
                     /locus_tag="QIE41_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDS3532516.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            4627..5550
                     /locus_tag="QIE41_003678"
     CDS             4627..5550
                     /locus_tag="QIE41_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDS3532517.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(5554..6372)
                     /gene="nlpA"
                     /locus_tag="QIE41_003679"
     CDS             complement(5554..6372)
                     /gene="nlpA"
                     /locus_tag="QIE41_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDS3532518.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITENPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            6582..6875
                     /locus_tag="QIE41_003680"
     CDS             6582..6875
                     /locus_tag="QIE41_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532519.1"
                     /translation="MKPTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            7177..7674
                     /locus_tag="QIE41_003681"
     CDS             7177..7674
                     /locus_tag="QIE41_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HDS3532520.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNIKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
     gene            7684..7866
                     /locus_tag="QIE41_003682"
     CDS             7684..7866
                     /locus_tag="QIE41_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532521.1"
                     /translation="MNINGKNASFTPEELIRKYHEEAPVDLKNNHGDKINEKNNNKHI
                     TYFPVDAILDGNKHLP"
     gene            7922..9703
                     /locus_tag="QIE41_003683"
     CDS             7922..9703
                     /locus_tag="QIE41_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HDS3532522.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSNKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAMQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYSSSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVVTQKSAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARSYTTWTKVLNIINEVVTVVTFAVSAALIVIG
                     IVAAVPSAGASFAGALALIGGIAGAVVLGVDIICQITLGTTATGWILGKLTEGLAGII
                     SKYDPTLLAICALLDALGVQQTTIDLVQDIYSSAAASIVMVTLMIGATLICSVAISEL
                     VKALSKTAAEEITKEITDSIKSTVESMIKSVSKKIMSALNFLGDKIKASSQFIMIFAK
                     ITILLEKIASGVCTIITSSMNQYAASKSADIEILQQDISNLSKTREQMLTVMQRIDKT
                     VEQEVAQMVRVLQHRTEALKFASHSIV"
     gene            9798..10847
                     /locus_tag="QIE41_003684"
     CDS             9798..10847
                     /locus_tag="QIE41_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532523.1"
                     /translation="MKQEYHKLKKILQSFAPNDIKVDTNTKFISLIESVRALSPELQC
                     KVLDGLNYAKNTLHNINQNSAIATKSSCQSTESIEKTITFNILDMLDNLTMRQQNIME
                     AFLRVFTSMYNASAKADASMSIVFEQLVHLQSSETKNASWANFAGALAGSAINATGAV
                     FMAGAELAKPAGKIDDIAENQEQNTDKPDSQTRIKSKEDMANAKARLAEIENQQKDLS
                     NNKNIEDNDKQEQSSKLQKEKESITNKIKDKEKINSHNLRYGITSSISHTNNLFQSIA
                     NLSQYGGTVAAEHTRAEKDLAEQAKNVMHSVMSSYLDQINRDVQGRDQALNLLQQIIQ
                     TIIDTDSAIVQGIRS"
     gene            10891..12240
                     /locus_tag="QIE41_003685"
     CDS             10891..12240
                     /locus_tag="QIE41_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HDS3532524.1"
                     /translation="MINKDNNLNIDILRQQNTNIPLKEASSQSEEPHQLENSKSEGNL
                     YNSASMDNNIGAMLKKDVEKLSTLVSERSPRNEDWKCQHDIQLNFVKNMLRNFPTENL
                     SEKGSALLHESYSLIKNVLQLTENVNTIDGKLKDPAKILTLHNSVRKTLANVADNTTG
                     SSAMASTKGTLNHAIASLDSSAPEVRSSATSSTLTASSSNEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNSQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT         3719 a         2766 c         2620 g         3348 t
ORIGIN      
        1 ctgaactgag tgggaattct gatgagcaga attatcgcca ctaccggacc attcttagcc
       61 gattttctgt aaggatttta tcgtgtcaga cacactcccc gggacaacac ttcccgacga
      121 caatcacgac cgcccctggt gggggctgcc ctgcaccgtg acgccctgtt tcggggcacg
      181 tctggtgcag gagggtaacc ggttgcatta ccttgccgac cgcgccggta tcagaggcct
      241 gttcagcgat gcagatgcgt accacctgga ccaggccttt ccgctgctga tgaaacaact
      301 ggaactcatg ctcaccagcg gtgaactgaa tccccgccat cagcataccg tcacgctgta
      361 tgcaaaaggg ctgacctgca aagccgatac cctcagcagt tgtggttacg tttatctggc
      421 tgtttatccg acgcccgaaa tgaaaaatta actctccaga atagccttct gctacggcct
      481 ggtgttttca ccacgccact tttccatttt tatatctgca tatcaggaaa atcttcagta
      541 tgaaaacatt acccgacaca cacgtacggg aggcatcgcg ctgcccgtct cccgtcacca
      601 tctggcagac actgctgtcc cggttgctgg accagcacta cggcctcaca ctgaatgaca
      661 caccgtttgc cgatgaacgt gtgattgagc agcatattga agctggcatt tcactgtgtg
      721 atgcggtgaa ctttctcgtg gaaaaatacg cactggtgcg taccgaccag ccgggattca
      781 gcgcctgtac ccactctcag ttaataaaca gcatcgatat cctccgggcc cgccgggcga
      841 caggcctgat gacccgcgac aattacagaa cggtaaataa cattaccctg ggtaaacatc
      901 cgggggcgaa acaatgaaac tggccctgac gctggaagcc gacagcatta acgtgcaggc
      961 actgaacatg gggcgcattg tcgttgacgt cgatggtgtt actcttgctg aactgattaa
     1021 cgtggtctgc gataacggtt actcccttcg tgttgttgat gaatctgacc ggacatcagc
     1081 agagcgcacg ccaccatctg cgacccttac cggcatacgc tgcagtaccg cacatatcac
     1141 ggaaacggac aatgcctggt tgttctcgct gtcacatcag acgaatgaca ccggcgaatc
     1201 agaatggatt catttcacag gtagcggata tctgttacgt accgatgcgt ggtcatatcc
     1261 ggttctgcgg cttaaacgcc tggggttgtc aaaaacgttc cgtcgtctgg ttgtcacact
     1321 catccaacgt tatggcgtca gtctcattca tctggatgcc agcgctgaat gtctgccgga
     1381 ccagcccact ttcgactggt aaccagaaac aacatgaaat cattaaccac ggaaactgca
     1441 ctggatattc tgattgcgtg gctacaggac aatatcgact gcgaatcggg aattatcttt
     1501 gacaacgatg aggatagaac ggattcagca gcactgttgc cctgcatcga acaggcccgg
     1561 aaggatgtcc gtgctcttcg tcatctgcag cttctgcacc agaaccggtg aatatcactc
     1621 accatacttc attcccctga cgccagcctg aatacagctg gcgttttcgt ttataaacca
     1681 aaaggaaaac cgataatgtc agacatcaca atctcccgtc cggaagtggt caccgggcat
     1741 acggacgtta tctgctccac cagtatccaa cacgttgtga cggtacggaa tgcagcactg
     1801 caacaaaccg aaacgcttat ccggcaactg gcagaaatct cagtgctgac agcaggcatt
     1861 ggcggcaaaa ccgccctgga ctgggcgatg aaacagaatt ttcgctgtgg ctgctggctg
     1921 atggaaaaac cggaagcagc aatgaaagcc atcacccgca atctcgatcg cgaactctgg
     1981 cgtgacctga tgcaacgttc cgggatgctg tcattaatgg atgcgcaggc ccgtgataca
     2041 tggtaccggt cactggagta cgataatttt ccggaaatca gtgaagcgaa cattctgagc
     2101 acatttgaac aactgcacca gaataaggat gaggtgtttg agcgaggggt aattaatgtt
     2161 ttcaggggac tgagctggaa ttacaaaaac aattcaccct gtaaatttgg cagtaaaatt
     2221 atcgtcaaca atctggtgag gtgggacaga tggggatttc atctgaacaa cggcccacag
     2281 gccgatcgac ttgccgacct ggaaagaatg ctgcatctgt tcagcggcaa accgatcccc
     2341 gacaaccggg aaaacatcac tattcatctg gatgagcaca tccggtctgt tcagggtaaa
     2401 gagtgctatg aagatgaaat gttcagcatc agatacttta agaagggctc cgcgcacatc
     2461 acgttcagga aaccagaact ggttgatcgg ctaaatgata ttatagccag acactatccg
     2521 gaaatgttac cttcacaata gagtactaag aattgattca attatgtatt ttagcttcat
     2581 tccttcatgc aacatcattt gggggttctt atgggggtta ctcatttatc caacacatac
     2641 aaattattta agatcaatgc attaatactt atattcgact cctgtgatct tccgccaaaa
     2701 tacctcttct gacgttctcc aatcccttgt aatactgtca atccagatat tttctactgc
     2761 tcaatattca atcggcttca ggaatacctg gtcaaaggtt ttgtgatgga cgatgagcgg
     2821 ctgaagaatc cgcctgtcgg ttcttctgtc ctcaacgcat ccgcgatatg cgcgccagca
     2881 tgtgccgtgt ttatttgcag gtgatttacc ccctgctggc tctcagccaa ctgtatcgcc
     2941 attaaggtga agatgttgtt tcatacgccc cagaacacct tttttatacg acgattctat
     3001 tcctttaaca gctggcgaaa aaaactgacc tgtaaacttc accaataatc agactgtcat
     3061 caagattaat tttttcattt aatatatcaa atcaacttgt tttattttta ctatcaaaaa
     3121 tagctcattg ccttactata attaatatat aaattcggtg attaattctt aacatgcttt
     3181 tgctaataat ctcaattgct ggccctataa tattgcgcta gcattgcttt ctggttgtat
     3241 cagcgatact caaaaattct taatacaata ctcattcgac tggtacttat ttgtaactca
     3301 gttatatttt gtcgcccggt gattcagaag aatgcaaaaa acggctacca ctccatcaaa
     3361 aatacttgat ctcactgccg cggcattttt acttgtcgcc tttctgacgg gtattgcggg
     3421 cgctctccag actcctaccc taagtatatt cctcgcagat gaactgaaag cccgtcctat
     3481 aatggtaggt tttttcttca ccggtagcgc tattatggga attcttgtca gtcaatttct
     3541 ggcaagacac tccgataaac aaggcgaccg taaattactg atcctgctat gttgcttatt
     3601 tggagtgctg gcctgcacgc tttttgcgtg gaatcgcaac tacttcattc tcctctctac
     3661 gggcgtactt ctgagtagtt ttgcttccac cgcaaacccg caaatgttcg ccctcgcccg
     3721 tgaacacgcc gacagaacag gccgtgagac ggtaatgttc agtacatttt tacgtgctca
     3781 gatctcgctt gcctgggtta tcgggccacc gctcgcttat gagctggcaa tgggatttag
     3841 ttttaaagtg atgtatctca ccgcagccat cgcatttgtt gtttgcgggc tgatagtctg
     3901 gttgtttttg ccatcaatac aaagaaatat tcctgtcgtt acccaacccg tagaaatttt
     3961 accctccacc cataggaagc gggatacacg gctacttttc gtggtttgtt caatgatgtg
     4021 ggcggcgaat aatctctaca tgataaatat gccgctattt attattgatg aactgcatct
     4081 aaccgataaa ctggctggag aaatgattgg tattgctgcc ggtctggaaa ttccgatgat
     4141 gttaatcgca ggctattaca tgaaacgtat tggcaagcgg ctattaatgc tcattgctat
     4201 cgtgagtgga atgtgttttt acgccagcgt actcatggcg acgactccgg cagttgagct
     4261 ggaattgcaa attctaaatg ccatcttcct tggtattctc tgtggtatcg gcatgcttta
     4321 ttttcaggac ctgatgcctg aaaaaatagg ctctgcgaca acgttatatg caaatacttc
     4381 acgcgtcggc tggattatcg ccggctctgt tgacggaatt atggttgaaa tctggagcta
     4441 ccacgcgttg ttctggctgg cgatagggat gttgggtatt gcgatgattt gcctgctgtt
     4501 tattaaagat atttagctca cctgtctggg ctaagaaaaa tcggagtggc gcaaacagag
     4561 ccatcctcgc catttttgct atcatgcctg catacataaa cgacaaaaca gtatgcagag
     4621 ggaaaaatgg gttccaccag aaaggggatg ctgaacgttc tgattgccgc cgtgttgtgg
     4681 ggaagttcag gggtctgcgc gcaatacatc atggagcaaa gccagatgtc gtcgcagttt
     4741 ttgactatga cgcgtttgat attcgccggt ttgattctgc tgacgctgtc atttgttcat
     4801 ggcgataaaa tcttttctat tattaacaat cacaaagatg ccattagtct gctgattttt
     4861 tccgtggttg gcgcgctaac cgtacagctc acttttttgc taaccattga aaaatcgaat
     4921 gctgccacgg caacggtgct gcaattcctc tcaccgacga ttatcgtcgc ctggttctca
     4981 ctggtgcgta aatctcgccc gggcattctg gttttctgcg ctattttgac atcgctgatc
     5041 gggacttttt tattggtgac acacggtaat ccgacgtcat tatcgatctc tcctgccgcg
     5101 ttattctggg gcattgcctc ggcatttgct gcggcattct ataccaccta tccctcaacg
     5161 ctaattgccc gctatggcac gttgcctgtc gtcggctgga gtatgctgat cggcggtcta
     5221 attctgttgc ctttttatgc cagacaaggg acaaactttg tcgttaacgg cagtttgatt
     5281 ctggcgtttt tttatttggt ggtcattggt acgtccctga catttagtct gtacctgaaa
     5341 ggagcacaat taattggcgg gccaaaagcc agcattttga gctgtgcaga gccattaagt
     5401 agcgcgctgc tctctttgct gttgctgggg atcacattca cattaccgga ctggctgggc
     5461 acactgctga ttctgtcatc ggtgattttg atttcaatgg attcccgtcg ccgcgccaga
     5521 aaaataaatc gtccggcgcg ggatgagtga gaattaccag ccaggcaccg cgccaccgtt
     5581 aaagatggtt tctgccgctt tagcgacttc ggatgattga taagattgca gaaattcctt
     5641 cacgttttct gcattcttat tatcttcccg tgctaccaga atattcacat acggcgaatt
     5701 cttatcttca ataaatacgc tgtcgtgcac cggagaaagc ccggtctgct gaatgtaagt
     5761 ggtgctgata atcgccacat caactttggg atcatccagt acgcgcggga gctgcgcccc
     5821 ctcgagttcc ataatctgca aatgacgagg attctcggtt atatccagcg cggtaggtag
     5881 taaccctttt ccctctttca gagtaattag cttctctttt tgcaacagta aaagcgcgcg
     5941 accaaggttg gtcgggtcgt tggggatcgc cactgtcgcg ccttctttta tttgcgccac
     6001 ggttttgatt tttttggaat aacccgccat ggggaagaca aacgtatttc ccaccgctac
     6061 cagtttataa ccatgcgcct gattatcttg ttcaagaaag ggtcgatgct ggaagacgtt
     6121 cgcatcaagc tcgccatgat ttgtcgcatc gttcggtagc aacgaaccgc taaatcctac
     6181 caattcaaca tcgaggccat atttctcttt tgccaccttt ttagcgactt ccgcgacatc
     6241 ttgttctgcg ccattaataa cgcccacttt aatgtgcttt tcatcgctgc tgctctggtc
     6301 gcaacctgcc agcagaattc ctgccagtaa taatgcggcc cccgcccgta gatgatgtgt
     6361 tgtcagtttc acttttttat ccttttttta atcgttaact gactataatg gcaagatcac
     6421 tccgatttaa aaaacgaaaa gctatcgaat agaagaaaaa ggtatataaa actggcactt
     6481 cttactgacc acgcaaacac ctttcgtaca gcatgatgag agcggcaaaa gcaaactaca
     6541 cttatgcttc attcgatgca catttgcaga aggttgccgc tatgaagcca acgatgctac
     6601 tcatgattac cgtattcctg actttcccag ccatttctca ggccgaatcg ccattcagtt
     6661 ctctacagtc ggcaaaagaa aagaccacgg tattgcaaga tttacgtaaa atttgcacgc
     6721 cacaggcgtc attatcagac gaagcgtggg aaaaattaat gttgtctgat gagagtaaca
     6781 aacaacatat ccgcgaagct attgtggcga tggagcgtaa taatcagaat aactactggg
     6841 aagcgctggg taaggtggaa tgcccggata tgtgaatttc gcacacctcc gcgctcaggc
     6901 cagcagccgc tgacttaagc tcgcacatca gtaaatcacc tgctttttca aattccaatc
     6961 aaaattgcac cagttcatca ggattgtgga taaatttccc tctacattgt tttcatttca
     7021 tcacatagaa aatcttatct cattgaaaca atacttgttt ttaatgcaga aaaggaagcc
     7081 caacggatgg gcataaaaat gagcgaattt tgatatcacc cccctctaaa aaatggtgaa
     7141 gtctgaacaa agaatgttcg aaaggagaga gtagagatgg aaagcgcaaa tctggattta
     7201 gaagaaaata aagaaattgc cagtaagttc gaacgtgcat taggtatggg agcaacactt
     7261 gcagagctgc atggcattac tccggatacc cttgaaggcg tttatgctta cgcttataac
     7321 ttttatgaaa aaggaagact tgacgaagct gagctctttt ttaaatttct ctgtatttat
     7381 gatttccaaa attataacta tttaaaagga tatgccgccg tttgtcagtt gaagaaagat
     7441 tatcaaaagg cattcgacat gtatcatatc tgtttaatgc tatctcccga taacgatttc
     7501 tctttggttt attatatggg ccaatgccag atgggcttaa aaaacattaa aatggccact
     7561 gagttattta atacagtcgt aacctacagc caaaatgaaa aagttaaaga gatggctact
     7621 gcttatcttg aactcttgac agccaatacc gaagaggaag ttcaaacaga gtgagtcggc
     7681 aaaatgaaca taaacggaaa aaacgcttct tttacgcctg aagagttaat caggaaatat
     7741 catgaggaag ccccggttga tttaaaaaat aaccatggcg ataaaataaa tgagaagaat
     7801 aacaataaac acattactta ctttcccgtt gatgccattt tagatgggaa taaacatctg
     7861 ccatgagtta aataaaaaag aattaattac acccgatgat agcttaaaaa ggaggtgaat
     7921 tatgtcggca ccgattacgg gacaaacgat cacgtttgaa caaatcagcg aaacattgcg
     7981 cacacagtat agcgatgcgg aaaaaagact gcaggacagc aacaaaacgc aagttgatcc
     8041 catgcggctt aataaaaacc cgaaatccct tgataatgat attcgcgctc gtcttgaaaa
     8101 caaaccgatg cttgctccgc ctgcaatgca ggtttcagat tccgacaacg ccactacagc
     8161 aaagacgaac gatgcccgcc tgacaatgat tttgggtaat ctaacaggca ttgccgatca
     8221 ggatattaca acacgtttgc ataataacct tgacagcacc ctgcttcgac acgaaatggc
     8281 acataataag tttcgtgaat taagtgacgc ttactcatcg tcattagatt ccgcgcaaaa
     8341 agccgacgac atcatgcatc aggccaataa taattacaat gcggtcgata aaaaggtgca
     8401 atcgctggag aaaaaagtca acacattgaa tcaagagctc tcgcaactgc aacctggcga
     8461 tccgcaatat aataaagtag tgacgcaaaa aagtgcggct gaaaaaacac tcacgctgtc
     8521 attacaaaaa aaatcgttag ctgagaaatc gttaaataca gccattatgg atgctgatgc
     8581 tgcgatcggc caaagtatgg aaatttttga cgaaattcaa cagcaagaac agattaataa
     8641 cttcaccacc aatatttgcc tgacgcaaga aaaccagaaa aacagaaacg cgacagccac
     8701 atttatcctt ttgattacct cagtaatgga agttattggt gataccaatt gcgactctat
     8761 taaaaatcaa tctgaggtaa tgaaagagat taaccatgtc agggaaaata aactcaatga
     8821 aacagcgcgt agctatacta catggacaaa agttttaaac attattaatg aggtcgttac
     8881 tgtagttaca tttgccgtaa gtgcagcctt aattgtgatt ggtattgtcg cagcagttcc
     8941 aagtgctggg gcaagctttg ctggagcgct ggcactaatt ggaggaatcg cgggggcagt
     9001 agtattaggt gttgatatta tttgtcaaat cactctgggt accacggcta ccggttggat
     9061 tttgggaaaa ctaactgaag gtcttgcggg tattatttct aaatatgacc ccacactact
     9121 cgcaatctgc gctcttctgg acgccttggg tgtccaacaa acgactattg atttagtaca
     9181 agatatttac tcatctgcag ccgcttctat tgttatggta acattaatga ttggtgcaac
     9241 attaatttgt tcagtagcaa taagtgaact tgtaaaggca ctctcaaaaa ctgccgccga
     9301 agaaatcaca aaagaaataa ctgacagtat aaaatccacc gtggaatcta tgatcaagtc
     9361 agtatcaaaa aaaatcatgt cagcattaaa ttttctcggt gacaaaataa aagcatcatc
     9421 acaatttata atgatatttg caaaaataac tattcttctt gaaaaaattg cctcgggcgt
     9481 ttgtacaata attacatcga gcatgaatca atacgctgcc agtaagtctg cagatatcga
     9541 aattctacaa caagatataa gcaatctatc aaaaacgcgc gaacaaatgc ttacagtgat
     9601 gcaaaggata gataaaaccg tcgaacaaga ggtagctcag atggtgcgtg tattacaaca
     9661 ccgaactgaa gctttgaaat ttgcttctca ttctatcgta taagaggatt caaaaatgac
     9721 caataatatt agcaatataa acaatacagc tcataataag aatctcgata aaacaacatt
     9781 cgatgaacaa cctgcttatg aaacaagagt atcacaagct aaaaaaaata ctgcaaagtt
     9841 ttgcgccaaa tgatattaaa gttgatacaa atactaaatt tattagtctg atagagtctg
     9901 tccgtgctct ctctcctgaa ttacaatgta aggtactaga tggtcttaat tatgctaaga
     9961 atacactaca taatataaat caaaattctg ccatagcaac aaaatcatcc tgtcagtcta
    10021 ccgagtcaat agagaaaacc ataacattta atattctcga tatgttagat aatttgacga
    10081 tgagacaaca gaacattatg gaggcctttc taagagtctt tacatcaatg tacaatgcct
    10141 ctgccaaggc tgatgctagc atgagcatcg tttttgaaca gcttgtccat cttcagagta
    10201 gcgaaacaaa aaatgcttct tgggcaaatt ttgcaggtgc attagcaggc tcagctatta
    10261 atgctacggg agctgtattt atggcaggtg cagaacttgc gaagcccgcg ggaaagatag
    10321 atgatatcgc agaaaaccag gaacaaaata cagacaaacc agatagccaa actcggataa
    10381 aatcaaaaga agatatggca aatgcgaaag ccagacttgc cgaaatagaa aatcaacaaa
    10441 aagatttatc aaataacaag aatattgagg ataatgacaa acaagagcaa tcaagtaagt
    10501 tacaaaaaga aaaagaaagt atcactaata aaattaaaga caaagaaaaa attaatagtc
    10561 acaatctaag atatggtatt accagttcta tttctcatac caataatcta tttcagtcta
    10621 ttgcaaatct atctcaatac ggcggaacag tggcagcaga acatactcgt gcagaaaaag
    10681 acttggctga gcaagctaaa aatgttatgc attcagttat gagtagctat ctggatcaaa
    10741 ttaatcgtga tgttcaagga agagatcaag cactcaattt attacagcaa ataatacaaa
    10801 ccattattga tactgatagt gcaatcgttc aaggcattcg ctcataaatt attgcaataa
    10861 tagttagtta tatttaagga ggaatggcac atgattaata aagataataa tctaaatata
    10921 gatattttac gtcagcagaa tacaaacatt cctcttaaag aggcatcctc acaaagtgag
    10981 gagcctcatc aactggaaaa cagcaaaagc gagggtaatt tgtataacag cgccagtatg
    11041 gataataata ttggcgctat gctcaagaaa gatgttgaaa aactatcaac tttagtcagt
    11101 gaacgtagcc cgcgaaatga agactggaaa tgccagcatg acatacaact caacttcgtt
    11161 aaaaacatgc tcagaaattt cccaactgag aacctgtcag agaagggatc tgcgctctta
    11221 cacgaaagtt attcgctgat aaaaaacgtt ctgcagctta cagaaaatgt taatactatt
    11281 gacggaaaat taaaggatcc cgctaaaata ttgaccttgc ataactctgt gagaaaaacc
    11341 ctggctaacg ttgctgacaa cacaaccggg tcaagtgcaa tggcatcgac caaaggtacc
    11401 ttaaatcacg ctatcgcttc tttagatagt tctgcacctg aagtgagaag ttcagcaacc
    11461 agcagtactt taacggccag tagttcaaac gaatttaaaa gtgataaata tatatgcagt
    11521 gatattgctg atttgatgag tgtgctcggt agcgactacc tcgaaattta tgcgaatagc
    11581 gttgagataa tgagcgcata ctggcaggac ttcagtgaac atatccaaag caatatggga
    11641 aaatggactc actctaataa aaaaggcgat gcgatagttt ttgacgtcaa tgcattccaa
    11701 aaagccttga tgcattttta ttatatcgat aaatatccta atggtgattt ccactatcat
    11761 tacaatccag actatgtttt ataccctccg gcacctgcgg acaaaattgg cgttccactg
    11821 gaagaagcag aaaaatggtg cgccgcttta ggacttccgg taattccacc cgatcccaaa
    11881 caccgcacac cttctccgat tgtcgaagtt gaaccgcaag gtagcggtct gtatgttatt
    11941 attcccaacc ctcagattat agacagtatg agtcagtctt cagactcaat ggtgcatagg
    12001 gacgataaag ggaaggaaaa aaatatatct aaagaattca ccggctacga aataagcacg
    12061 gctgaatacc aggcatggct ggcgggttat aacagccagg cagaaaacat gaaaaccgac
    12121 gtgcaggtta tcacgactaa atacagtaca gccaactcca cctacgatac aattatcaaa
    12181 ttattatctt caaccattac tgcgttgttt gattcagcaa aagattattt acgtttctga
    12241 ttaagtaaca tccacaatta tcgcccacta tctggtcccg aaaaatggga ccagcgtttc
    12301 atattattaa gcatcagctt tcattcattt cttcatcaca atcttacaaa atgagcgttt
    12361 tttgatagag caattctgcc aatactttat tttatattga tcaaattaat actatgaata
    12421 gagaaaacgg aaggagcgtt ctatgcaact gca
//
LOCUS       DAOTRK010000133        12414 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.134, whole genome shotgun sequence.
ACCESSION   DAOTRK010000133 DAOTRK010000000
VERSION     DAOTRK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.134"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(5..2185)
                     /gene="katG"
                     /locus_tag="QIE41_003686"
     CDS             complement(5..2185)
                     /gene="katG"
                     /locus_tag="QIE41_003686"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDS3532525.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(2514..3404)
                     /gene="metF"
                     /locus_tag="QIE41_003687"
     CDS             complement(2514..3404)
                     /gene="metF"
                     /locus_tag="QIE41_003687"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDS3532526.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(3786..5339)
                     /locus_tag="QIE41_003688"
     CDS             complement(3786..5339)
                     /locus_tag="QIE41_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDS3532527.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHDFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAKSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMDLTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATIYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQI
                     NGMRVKDIK"
     gene            5472..6602
                     /locus_tag="QIE41_003689"
     CDS             5472..6602
                     /locus_tag="QIE41_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDS3532528.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTFATEKGHFCPVCYGTKTICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITPPEIIETVALLVPFQGASGGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHESNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGVI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGTTYHGWWKNQWQLWVQGKNRKMVCALTGGNNSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCARRLISD"
     gene            complement(6566..8998)
                     /gene="metL"
                     /locus_tag="QIE41_003690"
     CDS             complement(6566..8998)
                     /gene="metL"
                     /locus_tag="QIE41_003690"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDS3532529.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(9001..10161)
                     /gene="metB"
                     /locus_tag="QIE41_003691"
     CDS             complement(9001..10161)
                     /gene="metB"
                     /locus_tag="QIE41_003691"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDS3532530.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            10438..10755
                     /gene="metJ"
                     /locus_tag="QIE41_003692"
     CDS             10438..10755
                     /gene="metJ"
                     /locus_tag="QIE41_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDS3532531.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            10815..11423
                     /locus_tag="QIE41_003693"
     CDS             10815..11423
                     /locus_tag="QIE41_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDS3532532.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVKQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            11568..12243
                     /locus_tag="QIE41_003694"
                     /pseudo
     CDS             11568..12243
                     /locus_tag="QIE41_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1H family transposase"
BASE COUNT         3018 a         3369 c         3041 g         2986 t
ORIGIN      
        1 cagattacag caggtcgaaa cggtcgaggt tcatcacttt cacccatgcc gccacgaagt
       61 ctttaacaaa cttctcgtgg gcatcgctgc tggcgtaaac ttccgccacc gcacgcagga
      121 cagagttaga accaaagacc agatccgcac ggctggcagt gtatttcact tcgccggttt
      181 cgcggtcacg gccttcgaac agctctttcg attcgtcggt cgctttccac tcgtaacgca
      241 tatccagcaa gttcacgaag aagtcattac tcaatacacc aacgcggtca gtgaagacgc
      301 cgtttttgct gccatcgaag ttggcaccca gtacacgcat cccgcccacc agcgcggtca
      361 tttccggcgc ggtcagcgtc agttgctgtg ctttatcaat cagcagtgac tcggtggtag
      421 aaacatccag acgagcgcgg tagttacgga aaccgtcagc aattggctcc agcagttcga
      481 acatttcaat gtcagtctga tcctgacgcg catcaacgcg acccggcgca aacggtacat
      541 gaatgctcaa acctgcggcg cttgcggctt tctcaacgcc aaccacacct gccagcacga
      601 tgatatccgc cagcgaagct ttgccagact ctttctgaat tttctccaga actggcagag
      661 cacgaacggc tgcggcgttc acatcccagt cgcgctgcgg cattaatgcc agacgtgcac
      721 cattggcacc accacgtttg tcgccaccac ggaaggtaga agcagacgcc caggccaccg
      781 ataccagttc gctgacagac agaccagaat ccgcaatcgc gaatttcaga tcgataatgt
      841 cctgctcggt cgggttgtag atcggctgcg gcagcggatc ttgccagatc agatcttctt
      901 tcggtacttc cggcccgatg tagcgagatt tcggccccat atccctgtgc gtcagtttga
      961 accaggcacg ggcaaaggct tcgttgaacg cctgcggatc gttgaggaaa cgacgagaaa
     1021 tcttctcgaa ctcaggatca aaacgcagcg tcaggtcggt caccagcatt gtcggtttac
     1081 gtttcttcga cggatcgaac ggatccggga taatttccgg tgcgtctacc gcttcgaact
     1141 ggattgcgcc agccggactg cgggtctgta cccactcata cttgaacagg ttctcgaaga
     1201 aatagttgct ccactgggtc ggcgtctggg tccagactac ttccagacca gaggtaatgg
     1261 catctgcgcc aacgccgctg ccgtaagtgc tcgcccagcc taaaccttgt tcttcaatcg
     1321 gtgcagcttc tggatcagga cctacatttg atgtcggacc ggcaccgtgg gttttaccca
     1381 gcgtatgacc acccgcaatc agcgccacgg tttcttcgtc gttcatgccc atgttgccga
     1441 aggtcgcgcg gatagctgct gccgcagaaa gcggttcgcc gctgtgatcc gggccttccg
     1501 ggttaacgta aatcaggccc atctcggttg cgcccagcgg tgctttcgcc agtgcttccg
     1561 gatgacggtg ggtcagccag gctttttcat caccccagtt aacatccaga tccggttccc
     1621 agacgtcttc acgaccggca ccaaaaccga aggtacggaa gccggagttt tccagcgcca
     1681 cgttacccgc gaggataaac aggtcggccc aggagatttt ctgaccatat ttctgtttga
     1741 ttggccacaa caggcgacgc gctttatcga ggcttacgtt atccggccag gagttcagcg
     1801 gtgcaaaacg ttgctgacca cgacccgcgc cgccgcgtcc gtcgatggaa cggtaagtac
     1861 ccgcgccgtg ccaggccata cgaataaaca gaccggcgta gctgccccag tcggcaggcc
     1921 accacggctg agattctgtc agcagggctt tcagatcttt tttcagaccg tagtaatcta
     1981 atttgctgaa ttctttgcgg tagtcaaagt cctcacccag cgggttagaa cgattagaat
     2041 gttggtttaa caggtcaaca cggagttgat ttggccacca gtcgcgcgtg gttgtgcccc
     2101 cacctgcgct ctggtcgtga ccgccctgat ggaacgggca tttgccggtg gctgtcgtgt
     2161 tatggatatc gtctgacgtg ctcatcaatg tgctcccctc tacagtgtta ccgttacgat
     2221 acacagcgtt agagagaagt tataattgaa ttaatccacg gatgcgatag ttgagatctt
     2281 acatattgtt ggttaaagag atgtagatca aattgatcgt aattaagact ttttgattat
     2341 gtccgaattc ggacaaatgc tttataaaaa agggttttag cttcatatcc ttcaagtaat
     2401 gagggagaaa acaaggctga caaccttatt ttcccgccct catttcgagg cagcattttg
     2461 tgctctgttt aaaatttgtg atcactgtgt gattttcaca aaagccacac tatttataaa
     2521 ccaggtcgaa cacccagcgt atggcaaatc gcgtaactca tttcagcacg gttaagcgta
     2581 tagaagtgga aatctttcac tccttcacgg cttaaaatct tcaccatatc catcgcaata
     2641 ttcgcgccca ccagtttgcg ggtttcggca tcgtcatcca gcccgtcaaa catttgcgcc
     2701 atccacgccg gaatacgcac gttggtcatg tcggcaaatt tcttcgcttg tttaaagttc
     2761 gagaccggca aaattcccgg aatgatttcc acatcaatgc ccgccgatac gcagcggtca
     2821 cgaaaacgca ggtagctttc gacatcaaag aagaactgag taatcgcgcg gttggctccg
     2881 gcatccactt tgcgtttcag attaagcaaa tccgcctgag cgctttttgc ttccgggtga
     2941 acttccggat acgccgccac ggatatatcg aaatctgcca cttcttttaa cagcgtcacc
     3001 aggtcagaag catacatttc tggcttaccg cttcccggcg gcagatcgcc acgcagcgcc
     3061 acgatatgac gaataccgtt attccagtag tcacgtgcaa tggtgcgcag ctcgtcgggc
     3121 gtcgcatcaa tgcaagtaag atgcggtgcc gcttccagac cagtgcgatc tttaatgcct
     3181 ttaataatgc tgtgcgtacg gtcgcgctcg ccggagttcg cgccataggt caccgataca
     3241 aacttcggtt tcaggctgct aaggcgatcg atggagttcc acagggtctg ctccatttca
     3301 ctggtgcgcg gcgggaaaaa ctcgaacgaa acgttaatct gcccctggac ttctgccaga
     3361 ctctgattca gggcatcccg ctggctggcg tgaaaaaagc tcatacctta cctcatcaat
     3421 cgcttgtagt tatatgttgt gttgtgaaca tctatccatt tagacgtcca gatgtaaaga
     3481 tgaaggaaaa gccgaggggc gtcaaccgaa aaatgaccaa tgatggtgag gaaaaatcag
     3541 ggaagttgaa aaaaactcat cttaattaga ggcgataaaa gatttctcta tagcgcaatc
     3601 cacctcgcac gttttgactg atattctgac aacacagcga cacctgggct tattcgaaaa
     3661 acaacgtcct gccaccggat ggtgatgcta aggcatctga ttcggcctgc gccctcgcgt
     3721 aggccgaata ctggatctgc aaaatcaact ggtaacaaac agatgaatca actcaatacg
     3781 tacttttact tgatatcttt cacgcgcata ccgttgatct gttcatccgt gatggtgtta
     3841 cccgctttga agtaatcaac cacggcgtga taaacgtaat aaccgcccgt tgtgttacgc
     3901 gctttccctt cggtaaaggc ggtaaaacca tctccaccat cagcaaggaa actctgagta
     3961 gcaatgtggt aaatcgtcgc atcttcaatg ggtttgccat tcagagtcag cgtgattacc
     4021 cgctgaccaa ccggcttact gctgtcgtac ttcatttcca ggcctttcga tacctgtaaa
     4081 acgccattac ttaaacttgc gccgtgttcc atcaaactgc gtaattgttt acccgtgaga
     4141 tccatcgtca ccagttcgtt gggaaacggg aaggtactga tgacgccgcc catcgtaatc
     4201 gcccctgcgg ggatctcatt gcgaatgccg ccagagttgg ttaacgccaa ttgggtgttt
     4261 ttacccgccg ctaccagtaa agcatcagcc gccaggttcc cgagggaagc cgattcacca
     4321 taggcacgtt ttaattcaac cggcgatttc gcgaccgttt gttgcaccac ttcatcaagc
     4381 tttttattcc agccgtcgat cacctgtttt gtttgcggat cgggcttcca ctcgtcggcg
     4441 taaatggttt taagctcgaa gtttttcacc gtaaaatcat gcggcttctc tttgtagtcg
     4501 agaaccagtt tgcccacatc aatcccgccg ctgtcagtgg agaggatgag cgtattgccg
     4561 actttaatcg gttccggcgt acccacatgc gcatgtccgg tgatcaaaat atccagccct
     4621 ttcacctgac ttgccgtctg aatatcttta tccagcgcgc gacgcacatc tgtgcctccc
     4681 atactggact ggcgggccgg aacaccttca tggatcaggg cgacggtcaa atcaaccttg
     4741 cctttaagtt catcgatata acgttgtagc catttaattt catcacgcgc ttcaatcccc
     4801 actcgcgttg ccgcagatac cgtgtcatta aaggcaaata caccgtgcaa acctataacg
     4861 cctattttca cgccgtcttt ttcgatgatg gtatatggct tatcccagaa tgatttactg
     4921 ctgttctgat aaaaaacatt gccctgcaca atagggaatt ttgcctgact caactgtaat
     4981 aatgtgttgt cccagccgtg gtcaaattca tggttgccta tcgtgacggc atcaaacggc
     5041 atagtattca taatatcgat aatagcctta cctttggtca ggctgctgat atatggcccg
     5101 gtaaaataat cacccgcatc aaaaaaccat gtcgctttat tttttgcttt ttcttgctta
     5161 accagcgtag tgatatttgc ccagccgcca atatcccgtt taccatcagc gatccacggc
     5221 actttataag gctcgacatg cgcatggagg tcgttggtat aaatgatggt gacatctttg
     5281 gcgcaagccc agaacggcaa tgttaatgcg atacccgcag caagtatttt aattttcata
     5341 aatagtccct ttcagtgatt taaaggattg ctacattacg ttctgaaaga gttatttatg
     5401 tgagagtaat cacatccgca ggggatagtt gccggacaaa atcaacgacc ttgtgaaatc
     5461 gttggtgata tatgtcgact ctgtttacga ttaatgcctg caagtcgttt ggttgtcgta
     5521 atttagggca accaacatcg acagattaca gctggccaga ctatcgactg ggttatccgg
     5581 cactgcactg tcgggcatgt ggaagttacc cgccgttgtt tgacgaacag caatttcgtg
     5641 actggttgtc agttcactta tctacttttg caactgagaa aggtcatttc tgcccggtat
     5701 gttatggcac aaagacgatt tgttatggcc acaatccaca ggggtcgcaa cgcattcaat
     5761 gtcgtaactg caaaaaagtc tggacaccta aacaatatca gaaagaaata acgcctcctg
     5821 aaataatcga aacggttgct cttctggtgc catttcaggg agccagcggc gggcagaaac
     5881 tgtatgtttt aatcagtttt gatgctcttc gcggtaatat tcttcatctt tctactaatt
     5941 acacgcagca tcaggctgga gaaagtctgc attatcgata cagagggaat gcagaaccag
     6001 agttacacga gagtaatatt gtacaacgcg ttgatatgcg cgaagcacaa tttctacgcc
     6061 gcagtcagtt tgatgagatt caatatggca gcgcagcgct caaacgcaat gctaaaggtg
     6121 ttattctgcg tccggttatc actgcacacg ggcatttcag agtactgaat attttattcc
     6181 ctacagtaaa aacacatgta atttcacacg agtgtttttt aagaggtgcc attattacgg
     6241 catgggctga tctgtttcgc caacagcaag gtgagatttg gtttattgaa gaggaaatag
     6301 ctgacgatac cgataatatg ccctggcgtt ttcagggaac aacttatcac ggctggtgga
     6361 aaaatcagtg gcaactctgg gttcagggca aaaatcgtaa aatggtatgt gcattaacgg
     6421 gcggaaataa cagcaaagca gaaatgctct ctttagcaac cagcagacat tttatcgact
     6481 ggctacataa acaagcagta tttactcaca gcgccccgct ttccgcaggg cgcgttacgc
     6541 aaatactgct ttctctggct caggattaca acaactgcgc caggcggtta atatccgact
     6601 gaatcgcccc tgcggtgacg tcgcgccccg cgccaggccc acggatcacc agagggttat
     6661 cgcgatacca gcggctttcg atggcaaaga cgttatcgca cggcagcagt gatgccagcg
     6721 gatggtcttc acgcaccgct tccacgccca cacgcgcttt gccattggca tcgaaacgcg
     6781 ccacatagcg cagcaccagc cccatttcgc gggccgcttc cagtcgttgc accatctgct
     6841 cgttaagttc atcgccgttt tcaaagaaat ggtcgatgct accgccttcg caatgggcag
     6901 gcaccagcga ttccacgcgc acctgatctg gttcgatgtc gtaacccgct tcacgcgcca
     6961 gaatcaccag cttgcgcatc acgtctttgc cggaaaggtc atcacgcgga tcaggctcgg
     7021 ttaagccctg ctgccacgcc tgatccacca gctcggtaaa cggcacgcta ccgtcgaatt
     7081 gcaggaacag ccaggagagc gtgccggaga agatcccact gatcgacaaa atagtatcgc
     7141 cgctgtcgat cagatcgcgc acggtatggt tgatcggcaa tcccgcaccg acggtggcgt
     7201 tgtacagcca gtgacgcccg gttttttcga aggcgtcgtg gatctggcga tatttattgc
     7261 tgtcgctggc tcccgccagt ttgttggcgc tgatgacgtg gaaaccgtgg ctggcaaaat
     7321 cgagatactg atcagcaagc tgctgactgg cggtaacgtc caacaccact aaatcatcat
     7381 acggatgggc gcgcatccac aggaacagcg actcttcatc ctgctcgacc gcttcatcgt
     7441 tgaagaaggc taacgcgcgg ctggcgtcca gcccgtcata gctcaacagg ctgcggcggc
     7501 tgtccaccac gcctgccagc acaaattcaa agccggtacg cgccgaaagc gtgctctgct
     7561 cacgggcgaa cagttccagc cagcgggaac cgatattgcc cttgccgaac aataccaggc
     7621 cgatgcgttt ttctgcgcgg aagacggact gatgcagccc ctgaatcagg ctttcggtcg
     7681 gtccggtgcg cagtactgcc accaggctga tgccgtcatc ggactgccag gtaaactcga
     7741 ccggttggcc tttcagttgc tgccagaagc ggtgacaatg cagcgggtta cgggtgacgc
     7801 ccgccccgac catcgccacc agcgccagcc cctggcgcag acgcagttcg ccaggtaatc
     7861 ccgcttcgtc gaggattttc agcgcactgt cggccacttc tgaggtgtag caaaattgca
     7921 gcaactggcg atcgttatgc acgccaactg ccagtggacg cacctgcgcg cgtttcagga
     7981 tctggtcgat ctctttatgc gccagtttga aatcctggct ggcgggcacc tgaaactcaa
     8041 tcaaacagac atcatcgtgg ctggtgacaa tacgcgcacc agtaccggag gccagcacgc
     8101 gttcaatgcg cgtggagcct tgatccggcg tgtagctaca gcgcagctgc aggtcgattt
     8161 cgctgccaga aactggctgt aaagtacggg cgtgaagaac gggagccgcc agacgcgcca
     8221 gttcgctagc ttcatccaga cgcagcaatg gcagcagaca ggcatctttc actttacgcg
     8281 gatcggcact gtataccccg gcgacgtcgc tccagatggt tacacgagaa acacccgcca
     8341 gcgcaccgat ttgtgtcgcg gaatagtcgg aaccgttacg ccccagcagc accgtttcac
     8401 cagcgttgtt acggctgata aacccggtca ccaccagacg tttacccgga tgttgtacca
     8461 gtagctgttg cagcaacggg taagaaagcc cttcatcaac ctgcggttgc gcagcgcgtt
     8521 cagcgcgtaa aaactcgcgg gcatcaagcc aggccgctgg cagcccttgt tgattaagta
     8581 ccgcagacat caggcgcgcc gaccacactt cgccgtggcc caccacttcc gcatacaccg
     8641 cgtcgttaat accgctgtcg agcagcgccg ccaggcgttc cagatcgctg acaaaagcgc
     8701 taatgaggct atcggcttct tcggcgggta acaggccgct aatcagatcg cactgatagc
     8761 gacgcagcgt ttgttgaacc tgatgcgcag agagacgatc ggtctggctt agtttcaacc
     8821 agttaatcaa ctggttagtg gtgctaccgg cggcggaaac caccatcata tcgtcaggct
     8881 gagagtactc cgccataatg cccgcgacac gcaaataaca cttcacatca gccagactac
     8941 tgccaccaaa tttatgcagt tgacgacctt tcgcccctgc ctgcgcaatc acactcattt
     9001 ttaccccttg tttgcagccc ggaagccatt ttccaggtcg gcaattaaat cttcgccatc
     9061 ttcaataccg gtggagatac gcagcagcgt ctcggagatc ccggcggcgg cacgcgcttc
     9121 tggtgccatg cctgcatgtg tcatggttgc ggcgtgagag attaaacttt ccactccccc
     9181 taatgattcc gccagcgtaa acaacgacag cccgcccagg aaacgacgca gcgtctgctc
     9241 atcgccatcc agttcaaaac tcaacattgc gccaaagcct ttttgctggc gcgcggcaat
     9301 ttcatgcccc tgattttccg gcagcgacgg gtgatacagt tttttcacca acggctgggt
     9361 ttgcagatat ttcacaatcg cctgcgcgtt gcgctgggcc agctccatac gcggcaccag
     9421 cgttcgcaaa ccacgtagca gcagatagct gtcaaacgcg ccgcccgtca cgccaatatt
     9481 gtttgcccac caggccagtt cggtgacaac gtccggatct ttagcaatca ccacgccggc
     9541 caccacgtct gagtgaccgt tcagatattt cgtgcatgaa tgcaacacca gatcggcacc
     9601 taatgccagc ggattttgta atgccgggct taagaaggtg ttatccacca cgctcaccgc
     9661 cccgacttcc cttgccagat ggcagatttt cgcaatatcc acgacgcgta acaatggatt
     9721 acttgggctt tctaccagta ccagtttggg gttttctgcc agcgctgccc gtaatgcctg
     9781 ttcatcgcct tgatcaacaa acaacacgcg atagcaaccg cgtttcgcca ggctgtcgaa
     9841 caggcgatag ctgcctccgt agcagtcgtg cggcgcaacc agcagatcgc caggtttcaa
     9901 aaagacggtc gttaccaggt gaatcgcgga catgccggta ttagtaagta ctgcaccagc
     9961 accgccttcc agttctgcca gcgcacgctg aaccacatcg cgcgttgggt tgccgcgccg
    10021 cgagtaatca tgcgcgcgcg gttcattaaa tccggtaaag ttataggtgc tggaaagatg
    10081 gatgggaggg acaacgcaac catactgttc gtcgtcattt aacccgctac gcactgcgat
    10141 ggtggcctgt ttacgcgtca tgtgatgaag ttccctgggc tttgtcggtg aaatgtcagg
    10201 caccagagta aacattgtgt taatggacgt caatacatct ggacatctaa acttctttgc
    10261 gtatagattg agcaaatccc aaatagccgt taaaattata tgcattatca cgccgacagg
    10321 tgcattacac gatgtcacgg tagcgcctgt acggtaaact atgcgggttt acggtcagta
    10381 cccacataaa ttgtgtggtc tggtctcaat ttattgacga agaggattaa gtatctcatg
    10441 gctgaatgga gcggcgaata tatcagccca tacgctgagc acggcaagaa gagtgaacaa
    10501 gtcaaaaaga ttacggtttc cattcctctt aaggtgttaa aaatcctcac cgatgaacgc
    10561 acgcgtcgtc aggtgaacaa cctgcgtcac gctaccaaca gcgagctgct gtgcgaagcg
    10621 tttctgcatg cctttaccgg gcaacctttg ccggatgatg ccgatctgcg taaagagcgc
    10681 agcgacgaaa tcccggaagc ggcaaaagag atcatgcgtg agatggggat taacccggag
    10741 acgtgggaat actaaagcgg aaaagagcgg cgcggagtta accagccgct tttttcatac
    10801 atggacgttt aacgatgaaa atcaggctac tgatcctcag cctgctggtt tctgtgcctg
    10861 catttgcctg gcaaccacaa accggcgaca tcatctttca gatctctcgc tcatcgcaaa
    10921 gtaaagcgat ccaactggcg acccattccg attacagcca caccggtatg ctggtgatac
    10981 gcaacaaaaa gccctacgtt tttgaagcgg tcggcccggt gaaatacacc ccgctcaagc
    11041 agtggattgc ccatggtgaa aagggcaaat acgttgttcg ccgcgttgaa ggcggactga
    11101 gtgttaaaca acagcaaaaa ctggcgcaaa cggcaaaacg ttatctcggc aaaccgtatg
    11161 atttcagctt ctcgtggagc gacgatcgcc agtactgttc ggaagtggtg tggaaggttt
    11221 atcagaatgc gctgggaatg cgcgtgggcg aacagcagaa gttgaaagag ttcgacctga
    11281 gcagcccaca agttcaggca aagctcaaag aacgttacgg caagaatatt ccgcttgagg
    11341 aaacggtggt ctcgccacaa gccgttttcg atgcgccaca actcaccact gttgccaaag
    11401 aatggccgct gttttcgtgg taatggatga taaaaaaaat aattcccgcc gtagtctgtt
    11461 tgttgctttg tagttgcacg acctttccgc aagctgtcgc tccggtaaac ggtgatgcct
    11521 ccaattagtt gaattaagga tataatactc gcttttaagg ttgtttcatg gtcacagtta
    11581 accttcattg tccccgttgc cagtccgttc aggtttaccg tcacgggcag aaccccaaaa
    11641 gacatgatcg attccgcggc cgtgactgtc accgggtatt tcagctcagt tactgttacg
    11701 agccccgtaa accaggagtt aaagaccaaa tcgctgaaat ggctttcaac ggtgccgggg
    11761 ttcgctatac tgcccgtacg ctgaaaatcg gcattaatac agtcatccga actttaaaaa
    11821 actctcgcca cgaaaaataa cgtcatcccc ggtcgctcat gctgatgtgg cactcatctg
    11881 cgaacttagt cagcaatgga gctttgtcgg cagtaaagcg cgacagcact ggctctggta
    11941 cgtgtacaac acgaaaactg gtggtgttct ggcctacact tttggccccc ggactgatga
    12001 aacctgtcgt gaactgctgg cgctgcttac ccttttacca tcggcatgtt gaccactgac
    12061 gaatggggta gttacgctag agagttacca aaggagaagc atctgactgg taagattttt
    12121 actcagcaca ttgagcgtaa caatctgacc ctgagaaccc gaatcaaacg tctggcccgc
    12181 agaaccatct gtttctcgcg ttctgtagaa cttcatgaaa aagtcatcgg tgcttttatc
    12241 gaaaatatat gttctactaa ttggagtcat cacccttctg atgagctttc cttggatgat
    12301 taccagactg aatacacagc caaagatttt tacctcattc tgctcttttt tctcaaatga
    12361 catatattat attttacatc atattcatag gcatatgaca ttcccccgcc cccc
//
LOCUS       DAOTRK010000134        12345 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.135, whole genome shotgun sequence.
ACCESSION   DAOTRK010000134 DAOTRK010000000
VERSION     DAOTRK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.135"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(171..434)
                     /gene="cedA"
                     /locus_tag="QIE41_003695"
     CDS             complement(171..434)
                     /gene="cedA"
                     /locus_tag="QIE41_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDS3532533.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYIPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            617..2878
                     /gene="katE"
                     /locus_tag="QIE41_003696"
     CDS             617..2878
                     /gene="katE"
                     /locus_tag="QIE41_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDS3532534.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPTAEPTP
                     PGAQPTTPGSLKAPDTRNEKLNSLEDVRKGSENFALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     MWSRIPKIDKIPA"
     gene            complement(2925..3674)
                     /gene="chbG"
                     /locus_tag="QIE41_003697"
     CDS             complement(2925..3674)
                     /gene="chbG"
                     /locus_tag="QIE41_003697"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDS3532535.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRADRQMVFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSAYCL
                     PRLTELDVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3687..5039)
                     /gene="celF"
                     /locus_tag="QIE41_003698"
                     /gene_synonym="chbF"
     CDS             complement(3687..5039)
                     /gene="celF"
                     /locus_tag="QIE41_003698"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDS3532536.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5143..5985)
                     /gene="chbR"
                     /locus_tag="QIE41_003699"
     CDS             complement(5143..5985)
                     /gene="chbR"
                     /locus_tag="QIE41_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDS3532537.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5993..6343)
                     /gene="chbA"
                     /locus_tag="QIE41_003700"
     CDS             complement(5993..6343)
                     /gene="chbA"
                     /locus_tag="QIE41_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDS3532538.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6394..7752)
                     /gene="chbC"
                     /locus_tag="QIE41_003701"
     CDS             complement(6394..7752)
                     /gene="chbC"
                     /locus_tag="QIE41_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDS3532539.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7837..8157)
                     /gene="chbB"
                     /locus_tag="QIE41_003702"
     CDS             complement(7837..8157)
                     /gene="chbB"
                     /locus_tag="QIE41_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDS3532540.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8456..8794)
                     /gene="osmE"
                     /locus_tag="QIE41_003703"
     CDS             complement(8456..8794)
                     /gene="osmE"
                     /locus_tag="QIE41_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDS3532541.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8996..9823
                     /gene="nadE"
                     /locus_tag="QIE41_003704"
     CDS             8996..9823
                     /gene="nadE"
                     /locus_tag="QIE41_003704"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDS3532542.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10053..10940
                     /gene="cho"
                     /locus_tag="QIE41_003705"
     CDS             10053..10940
                     /gene="cho"
                     /locus_tag="QIE41_003705"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDS3532543.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10900..11475)
                     /gene="ves"
                     /locus_tag="QIE41_003706"
     CDS             complement(10900..11475)
                     /gene="ves"
                     /locus_tag="QIE41_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDS3532544.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11678..12163)
                     /gene="spy"
                     /locus_tag="QIE41_003707"
     CDS             complement(11678..12163)
                     /gene="spy"
                     /locus_tag="QIE41_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDS3532545.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         3248 a         3126 c         2829 g         3142 t
ORIGIN      
        1 aaacttaacc attttaaaat aaataaacaa ttaaagaaaa aagttcactt atttatagca
       61 atagatcgtc aaaggcagct ttttgttaca ggtggtttga atgaatgtag caatgaaata
      121 caaaatttca ggccatgtaa ctcccggcaa aaccgggagg tacgtaatct ttactcagtc
      181 acttcccctt cctggcggat ctgatttgcc caacgctggg cggattcagg cacagtaaat
      241 gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt gccacgcagc
      301 atccagacat cacgaaacga atccatcttt atcgcatgtt ctggcggcgc gggttccgtg
      361 cgtgggatat agctaataat ctggcggttt tgctgacgga gcggtttctt cattactggc
      421 ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta gcccctgtac
      481 gtcccgcttt gcgtgtattt cataacaccg tttccagaat agtctccgaa gcgggatctg
      541 gctgatggtc tatagttaga gggttttttg accaaaacag cggccctttc agtaataagt
      601 taaggagacg agttcaatgt cgcaacataa cgaaaagaat ccacatcagc accagtcacc
      661 actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg aagacggatc
      721 tcatcgtcca acggctgaac caacaccgcc aggcgcacaa cctaccaccc ctgggagcct
      781 gaaagcccct gatacgcgta acgaaaaact taattctctg gaagacgtac gcaaaggcag
      841 tgaaaatttt gcgctgacca ctaatcaggg cgtgcgcatc gccgatgatc aaaactcact
      901 gcgtgccggt agccgtggtc caacgctgct ggaagatttt attctgcgcg agaaaatcac
      961 ccactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat cagccgctca
     1021 cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt tcctctcaga
     1081 tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg gtgctggctc
     1141 tgccgatacc gtgcgtgata tccgtggctt tgccaccaag ttctataccg aagagggtat
     1201 ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc ataaattccc
     1261 cgattttgtt catgcggtaa aaccagaacc gcactgggca attccacaag ggcaaagcgc
     1321 ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca acgtgatgtg
     1381 ggcgatgtcg gatcgcggta tcccgcgcag ttaccgcacc atggaaggct tcggtattca
     1441 caccttccgc ctgattaatg ccgaagggaa ggcaacgttc gtacgtttcc actggaaacc
     1501 actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactaaccg gacgtgaccc
     1561 ggacttccac cgccgcgagt tgtgggaagc catcgaagca ggcgattttc cggaatacga
     1621 actgggcttc cagttgattc ctgaggaaga tgaatttaag ttcgacttcg accttcttga
     1681 tccaactaaa cttatcccgg aagaactggt gcccgttcag cgtgtcggca aaatggtgct
     1741 caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc atccaggtca
     1801 tattgttcct ggtctggact tcaccaacga tcccctgttg cagggacgtt tgttctccta
     1861 taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc cgattaaccg
     1921 cccgacctgc ccttaccata atttccagcg tgacggcatg catcgtatgg ggatcgacac
     1981 taacccggcg aattatgaac cgaactcgat caacgataac tggccgcgcg aaacaccgcc
     2041 ggggccgaaa cgcggcggtt ttgaatcata ccaggagcgc gtggaaggca ataaagttcg
     2101 cgagcgcagc ccatcgtttg gcgaatatta ttcccatccg cgtctgttct ggctaagtca
     2161 gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa gcaaagtagt
     2221 tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc tcactctggc
     2281 ccaggcggtg gcgaaaaatc tcggtatcga gctgacggac gaccagctga atatcacccc
     2341 gcctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg ccattcctga
     2401 cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga gatcggcaga
     2461 ccttctggcc attctcaagg cgctgaaggc caaaggcgtt catgccaaac tgctctactc
     2521 ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg ctacctttgc
     2581 cggtgcacct tcgctgacgg tcgatgcggt tattgtccct tgcggcaata tcgcggatat
     2641 cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc ttaaaccgat
     2701 tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg accagggtga
     2761 agaagggatt gtggaagctg acagcgccga tggtagtttt atggatgaac tgttaacgct
     2821 gatggcagca caccgcatgt ggtcacgcat tcctaagatt gacaaaattc cggcgtaaat
     2881 atggataagg tcacgccccc tggggagtga ccttaccacc ttacttacac atcaagataa
     2941 ctacccaacc gataaccacg ctcagcaatc gcatatttca acgacgctga agtcagcaca
     3001 tccagttccg ttaaccgagg taagcaataa gcactctggc gaatcgtatt atcgataaac
     3061 gccggatgac acatcacttc cagagagcgc tcgccgcgat gactggagtc atcaagcact
     3121 tgcaggaaca gcgactcact gatctcttca ccgtagaatg cactgctgaa tccctgggta
     3181 gtgcgcaggt tgaccggcaa atcaaacacc atctgacgat ccgcccgcaa cgcaatgccc
     3241 tcttctgccg caaacctggc aacaatcggg aaaatctgcg ggaacatatg cacatgatga
     3301 tggctatcaa gatgcgtagg tttgcgtcca aatagctcaa tgaaacgcaa atactgactg
     3361 gcaagctcct gagtaatttc ttccagcggt aaagcatctt cttctgccaa ctgccagatc
     3421 cattttccca gcacaccatc gcgggttaac cccggcatag ctgtcagtgg cttgcccata
     3481 gtaaggacaa agtgcatccc tatggccaga ctcggttcat cacgactcaa ctgcaccgca
     3541 tggtcaatag cctgcccatt caccagcgcc gtcgtcgatg tgacaatccc attgcgacag
     3601 gcctcgataa tgccgtagtt ctgacctttg cttaagccaa aatcatcggc attaacaatc
     3661 agtaagcgtt ccataatcag cctcggttaa tgtgcttttt taagctctgc gatgcagtcg
     3721 gcaaaatttg gcagccattt ctcgtgcgcc agaatcatct cgcgtgccag cagctcagca
     3781 tcgcgatcgg aatgcaccaa cggactaagg tttagcgcca gtaacacatc gttaaattct
     3841 ccgctaagtg cagcgttgct ggcagcaatc tcgaagcctt taatggtgtg aatcagcccc
     3901 atcactttat catcgaaatg cgtaatgcgc ggatgtggcg tcgcgccatc gcgccccagc
     3961 gtacaggtca tttctaccgc ccagtctgcc ggaatattat caatatgccc atgatgcggg
     4021 atattaacgt aatgttcagc ttgcttgtcg ttgtagatag cgttgatcac ttcgcacgct
     4081 gcatcagagt aataagcccc accgcgctgt tccagttctt tcggcttaac tttcaactcc
     4141 gggtttttat acagctcaaa aagttgtttc tcgactttct gtactacctg tgctcgtgcg
     4201 ccgcctttgt agtattcgcc catttcaata gccagcatct ctttttgctt gaagtaataa
     4261 agcaaataag aacacggcaa cagattcaga gaacgaatta agccttcgct aaatggcaga
     4321 tcgaaaatat ttttaacgcc agatgctttt aactgcccgg atgccacacc atcaagcaat
     4381 tcggcaaagc gtgatttgcc atttaccagc acatctttaa tgaacaccat atggttgagg
     4441 ccgaacagat cgatagataa atcatcgctg tctttcagca tcagaacatc gcgaataaac
     4501 atcttcatgc cgatcggaat attacacacg ccgataaagc gtttaaatcc ggtatgacga
     4561 taaacggctt cagtgaccat tcccgccggg ttagtgaagt taatcaccca tgcattcgga
     4621 caaagttctt cgacatcttt tacgatgtca aaaatcaccg gaatggtacg cagaccttta
     4681 aaaagaccgc ccgcgccgtt ggtttcctga ccaagataac catgacttaa tggaatacgt
     4741 tcatccagtt cgcgcgccgg taattggcct acgcgcagtt gggtagtaac gaaatcagca
     4801 tctttcaatg cttcgcggcg atccagcgtt ttataaagct tcatcgggac gccagcgtta
     4861 tcaatcatcc gttggcagag atcaaaaata atatccagtt tagctttacc accttcgaca
     4921 tccaccagcc ataattcgct gaccggcaat tcgtgataac gcttaataaa tccttccagt
     4981 aactccgggg tatagctgct cccgccacca atagtgacga cttttaattt ctggctcata
     5041 atttctccct tcagtacaga atactgatat ctggcatatc tgccccccgg acataaataa
     5101 tccagcaaca ggacagatat gtgaattgtc aggtataacg acttactgat taaattcagt
     5161 caatttctta cgatagctct taggcgtaaa ggacgttaat ttcttaaacg ttttaataaa
     5221 caagctcggg ctactataac cggcctcaaa cgcaatatcc gtcacggaat agttggtcat
     5281 ttccagttgt tttttggcaa aattaatacg gatctcatta ataatctgca ttggtgtttt
     5341 gccataatat cgttgagtcg ctcgcgtcaa atattcctgt gatttggctg acaacgtcac
     5401 catattctcc agcgccgatt cactaaactg ctctttatca tgcatctttt ctaccgtact
     5461 tttcagccac tgcggtacat catcaatcac ctgttcttcg cggtaatgac gtaaacggtt
     5521 aatgacataa aaagtaacca tctcaacaaa ctcttccagc cctgtttcgc ggaaattcaa
     5581 tgaagaaatc actgtttcca cataggtgag aaaagcgtta ttggtccggt atacctgcga
     5641 agcgacaaag caataaggca acaacggcag gtaatgctgc tcaaaaaagc gtttactgat
     5701 cccaacattc aatatgcgcg tggcaccaaa ctcataaaaa ctttgatggt gcgaacctaa
     5761 cggaataaaa acaaaatcgc cccgttccag taacacgcgc ttaccgttaa tctcctggaa
     5821 ataacgcccg gttaatacca gagtaaattc ataatagtcg tgctgatgca gtccgctgat
     5881 actctcagtt ttgttgtaga taaacacatg gaagtttttg ccattaaaca actgctgttc
     5941 tcgggcagtg gcaatttccg gcgcgttaat cactggctgc atcatcgact ccttatgcct
     6001 tcagtttttc atgaagctca attaattcgg taatcagttc acgcgcaagc atggacgtca
     6061 ttaaatgatc ctgagcgtgg accagcacca gactcacttt catcttacct tcgcccgcat
     6121 cgccttcaat cagtttcgtc tgtaccagat gcgcttcatt caatgccatc cgtgactgat
     6181 ccatcatggc ttttgctgcg gcaaaatcgc cctgcttcgc ctgtttcagt gcggcatacg
     6241 ccaggctgcg cgcttgtccg gagttgatga taagtcccat caccacttct tccagctctt
     6301 cagcttctgt ttgcgtatcg ggaatgttat cgagatccat catacatcgt tcctctcttt
     6361 tcttaccggc acgattaccc gtaccggcat cgattaaaat ttcagggcgt tagcgatatc
     6421 ttcttcgctc tcttctttat caatcgcgtt ttgtgctttg ttagcgacca caacaaaggg
     6481 cagataaatt aacgttgcga taccaaggtt gaagagtgcg accagcaatg cggcgacgct
     6541 accgttggtg ttaaagaagg ctcccagacc ggttggcatg gtccacggtg caatattggt
     6601 caccggagga ataatgccca tgtagtaagc tgcgagggtg attgccgcca gaatcggttg
     6661 taccagtaca aacggaataa acatcaccgg gttcatgata attggcagac caaacagaat
     6721 cggttcgtta atctggaaga tgccggacgg cagcgccagt tttgccacct gacgataatc
     6781 agcacggcga gaagcgataa agatagccag gatcagaccc aaagtcgcac cactgccacc
     6841 gaggaaaata aaggagtcca gcatcggctt ggcccagata tggaaggtct taccggctgc
     6901 cagcgccgct tcgacggaac catattgctg ataggtcgcg atgttttcca gcgcccacgg
     6961 cgtcataatg ccgttgtcca gtgcggtcag cgccagcgcg ccatgaatac cgaagaacca
     7021 gagcagtgga acaaagatca cataggccca tcccaccacg ctacccaacg atgccagtgg
     7081 ggttgagatg gtatccataa tgatctgatg gaaattggta ccccaggtat tcaacgccca
     7141 ggcaataatc cccatcacgg aaagaataat aaagccgggg attaatgccg agaaggaacg
     7201 cgatactgaa gcaggtacgc tgtcgggcaa tttaatgacc caattgcggt ggacaataaa
     7261 ggtaaacatt tctgccacca ccaggccaat aataatcccg gagatgatat tcgccccacc
     7321 taaccagttt gcgccaaccg cataggcctc accgacactg tatggggtga cggtcataaa
     7381 tgctgcaacg gataacaacc cagccgccag cgcatcgact ttacgctctt ctgccagcgc
     7441 catgccaata aagaacggtg ccattaaaga cattatccct aatgttccgt tatatacgtt
     7501 gccgccaata cctttcagac cattaagtgt ttcaatggtt gaggcatcca ggcgaatacc
     7561 taaggaataa aaaaacgacc cctccccaaa gcttagaaaa acgttgttaa ttaatacaaa
     7621 catggccccc gcaagggtta acggcattaa gcgaataaag ccatttttga ttgcattaac
     7681 gtgtggctgc tttcctattt taactgcaaa agggaggagt accttttcaa gcgatgcaat
     7741 aacattactc atagaaaaat acccttaaaa accgcaattt aaatattgcg gtattgattt
     7801 atgaaataac tctttgacgg gaaaatttaa aataaattaa tttgctgcgg cttttttaat
     7861 cgctgcaacc gcagccttaa gcacgcctaa accatcgact ttgccataaa gcagcgagtc
     7921 aattacttca accggtttgt tgggtaacaa acgctggatt tcgggcaaca tataagcaat
     7981 ctgcggccct aataacacga catcggcatt ctgacctttt tcaccagcca gtgtttccgg
     8041 aaatgcttca ataatgaccg gaacttcata tttttctgcc tgtgcgcgca tttttgatac
     8101 cagtaaagag gtagacatgc ccgcagaaca aaacagataa atgtgtttct tttccataaa
     8161 actgccctca tcgacgatta tctgtcagcc agacactccg caagccttaa cctgcttcca
     8221 tgctctgggt aacttgcgaa accaacatga ctttttttgt ggctgaaagg agtatacggt
     8281 atagacctga gcaatagtat ttctttgacc ttataaattg tctctccttg acctgcggtt
     8341 atgaccctct tcacatttcg ggcaaataat tcgcgaagat aattaagaac tcaggccaaa
     8401 aaaaaccggc gcaatggccg gtttccgttg taactcaagc tttcaggcga agtgattact
     8461 tcgcagcctg tggatcagtg tcgtattcag cacaggtctg ataaccggag ttgatgacat
     8521 gtccggtatc atctaacgcg acaaagtagg tttctgcttt accatcacgt tgacccagga
     8581 tgtaggtctg gcaagtacca cgagcatgga tcatgctcac ttcagacgaa ggtttacccg
     8641 caatttgtgc aacctgcgcc cggctcatgc cttttttgac gtctttcacc acaggctgta
     8701 caaactggtc tttggtacga tcataagccg tacaacccgc cagcatggtt aataccgccg
     8761 ctgcactcag aattcctgcc atattcttgt tcatattccg tcctcttgtt tatcagcgtg
     8821 ttagataagc ctggaataca ttgggcgctt tttcaagccc gtgaacgaaa cggctccgct
     8881 ttcagaggat tcctgtatga cgttttaacc accattcaac ccgctgtcgc ttgtcgtttc
     8941 agtagcaacg ggttagcttt aaggaagttt tgtcttttct gtctggaggg gttcaatgac
     9001 attgcaacaa caaataataa aggcgctggg cgcaaaaccg cagattaatg ctgaagagga
     9061 aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa acttatccgt tcattaaatc
     9121 actggtgctc gggatcagcg gcggtcagga ctccacgctt gccggaaagc tgtgccagat
     9181 ggcgattaat gagctgcgcc aggaaaccgg caacgaatcg ctgcaattta ttgccgtacg
     9241 cctgccctat ggtgttcagg ccgacgaaca agattgccag gatgccattg cctttattca
     9301 accggatcgc gtattaaccg ttaatatcaa gggcgcggta ttggccagcg aacaggcgct
     9361 gcgcgaagcg ggcattgaac tgagcgattt tgttcgtggc aatgaaaaag cgcgtgagcg
     9421 gatgaaagca caatatagca ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca
     9481 tgcagcagaa gccattaccg gattcttcac taaatatggt gacggcggta cggacattaa
     9541 tccactgtat cgtctcaaca aacgtcaggg taaacagtta ctggcagcat taggttgccc
     9601 ggaacacctt tataagaaag ccccaactgc cgatctggaa gatgatcgcc cttctctgcc
     9661 agatgaagtg gcactcggcg tgacctatga caatatcgac gactatctgg aagggaaaaa
     9721 cgtacctgaa caggtcgcca gaacaataga gaactggtat ctgaaaactg aacataaacg
     9781 ccgtccgcca attaccgttt tcgatgattt ctggaaaaag taataatttg cacatattgg
     9841 tttgtgcgaa aaagagtact ttgttcacgc cggatgcggc gtgaacgcct tattcgcccc
     9901 acaaaactat gcaaattcaa tatattgcat gagtcgaata ggcctgacag gcgtagcacg
     9961 tcagacggtg taacctttgt catcgacccg cctctttttt aatcgctccc cgcctgttac
    10021 actggataga taaccagcat tcggagtcaa cagtggtacg gcgtttaact tctccgcggc
    10081 tcgaatttga agctgcggca atttatgaat atcccgaaca tttacgttca ttccttaatg
    10141 acttacccac ccgaccaggg gtgtatctgt ttcatggcga aagtgacacc atgccgctct
    10201 atatcggcaa aagcgttaat atccgcagtc gggtgctttc tcatttacgc accccggatg
    10261 aagccgccat gctacggcaa tcccggcgaa ttagctggat ctgcaccgca ggcgaaatcg
    10321 gcgctctgct ccttgaagcg cgattaatca aagaacaaca accgctgttt aataaacggt
    10381 tgcgccgcaa tcgccagctc tgcgccctgc aattaaatga aaagcgtgtt gatgtggtgt
    10441 atgccaaaga ggtggatttt tcacgagccc ccaacctgtt tggcctgttt gccaataggc
    10501 gcgcagcttt gcaagcattg cagaccatcg ctgatgaaca aaaactttgc tatggcctgc
    10561 tgggactgga accgttaagc cgcgggcgtg cttgttttcg ttctgcatta aaacgttgcg
    10621 ctggggcgtg ttgcggtaaa gagagccatg aggaacatgc gctacgtttg cgccagtctc
    10681 tggagcgttt gcgggtggtg tgttggcctt ggcaaggggc ggtggcgctg aaagaacagc
    10741 acccggaaat gactcaatat catattattc aaaactggct gtggctggga gcggttaatt
    10801 cgctggaaga agcgacaacg ttaattcgga cacccgccgg gtttgatcac gacggttata
    10861 aaattctttg taagccgctg ctttccggta actatgaaat tactgaactt gatccggcga
    10921 atgaccagcg agccagttga tttcactgaa caacagttta ccctgcggtt gtaaaagccg
    10981 cagggtgtgc ctgccatcga accagcacgc gccctggtcg gtagtgagca gtttgtcgcc
    11041 cagttgccac gcgccgttaa ttacaaacac caccccgccg cgtgagccaa aggtagtaaa
    11101 cgtacgttcg gcaattctga ctttcgcctt acagacatcc aggcgtgtca tgatgttgaa
    11161 atccatcgac atctgccctg ccgtcagttt cgcttttacc acctggtccg ccgcaaaggc
    11221 aaaaggctgt aacggtttta aggtatggtt aaagcggtct gcgctttcaa ggaacatctc
    11281 gccgccttcc agcaacgtca ctatcctttc catacctgga aacaaagaaa actccccatt
    11341 cgccgcgatg gatgcaatgc tggcacgcca gtaaaaatca cgttttgccg gtgggaaagt
    11401 gcaaatttcg cgcgtttcac cggcagcatt tcgccacaga ttcaccgaca ttttacgcat
    11461 atcaaagtat tccatactcg ctcccggccc gttcttttca ttgttatcgt tcaggtaacc
    11521 gtggatggtt atcggcaact cgggtgattt acgttagtgg cgatcaggct ttttttgcat
    11581 ctgtttgaga acaaaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg
    11641 gacaagaccg gcggtcttaa gttttttggc tgaaagatta ttcagcagtt gcaggcattt
    11701 taccttttgc cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt
    11761 tctgttccgg cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt
    11821 tagctttgcg ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg
    11881 tatcgctggc aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct
    11941 ggtcacgctg gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca
    12001 ggtctttgaa catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct
    12061 tcgcgtcagc cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa
    12121 gagccagggt agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt
    12181 tcagttattt attacggctt tcttaagaag cgtgccgtgt tgacgagatt aactttactg
    12241 actttagcgt caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg
    12301 cgccaattat ggagaaaaaa agaaaatttg atggagagtg atgag
//
LOCUS       DAOTRK010000135        12335 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.136, whole genome shotgun sequence.
ACCESSION   DAOTRK010000135 DAOTRK010000000
VERSION     DAOTRK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.136"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            306..1250
                     /locus_tag="QIE41_003708"
     CDS             306..1250
                     /locus_tag="QIE41_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532546.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSVP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            1331..1753
                     /locus_tag="QIE41_003709"
     CDS             1331..1753
                     /locus_tag="QIE41_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532547.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFPILSSLIDSAISKFQHQIPVNGSVNPDLLYEDYTD"
     gene            2300..2749
                     /locus_tag="QIE41_003710"
     CDS             2300..2749
                     /locus_tag="QIE41_003710"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HDS3532548.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRECCIERGLGNILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFSPSHDLEDVCQLMAHDINQRHNVDTRILEEAI"
     gene            2749..4137
                     /locus_tag="QIE41_003711"
     CDS             2749..4137
                     /locus_tag="QIE41_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDS3532549.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVSVNFAKQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            4156..5601
                     /locus_tag="QIE41_003712"
     CDS             4156..5601
                     /locus_tag="QIE41_003712"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HDS3532550.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAALGIKKSIEAGTSKLNGLPVVNHGVAACRRLTEALQKPLQIRHGTPDARLLAEI
                     SMSSGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEACGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            5601..6842
                     /locus_tag="QIE41_003713"
     CDS             5601..6842
                     /locus_tag="QIE41_003713"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HDS3532551.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            6846..8216
                     /locus_tag="QIE41_003714"
     CDS             6846..8216
                     /locus_tag="QIE41_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HDS3532552.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALEFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDSEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            8213..8530
                     /locus_tag="QIE41_003715"
     CDS             8213..8530
                     /locus_tag="QIE41_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HDS3532553.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            8691..9971
                     /locus_tag="QIE41_003716"
     CDS             8691..9971
                     /locus_tag="QIE41_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDS3532554.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            10079..11731
                     /locus_tag="QIE41_003717"
     CDS             10079..11731
                     /locus_tag="QIE41_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDS3532555.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGNEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            11741..>12335
                     /gene="cobO"
                     /locus_tag="QIE41_003718"
     CDS             11741..>12335
                     /gene="cobO"
                     /locus_tag="QIE41_003718"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDS3532556.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGI"
BASE COUNT         3298 a         2971 c         2961 g         3105 t
ORIGIN      
        1 actttttata ataccaattg attagagtag ccaatttgtt gctatttcac ataacatatt
       61 tttatcaaag ttaattgatt gttgccttta tgagcaccga ccatatgccc gttatgttat
      121 tgtttcatta gtaataatca cttaaatgta tcgatgaaac aggtatgact tagcgggagt
      181 gattgtttct gagtgaacag aggccacttt cgcctgataa atgaaaaatg agcgattttt
      241 gcaagttagt ttttctatag ccgagtacgc tcagcgcctc gcttgattac aatgaggttg
      301 ctgctatgag tcctgataat atcggttcgg ctgtcaatcg cttctcaggt tggccatcta
      361 atgattctct aaaaacagat aaagaatcaa tgacatcacc cgtgactgtg caggtaataa
      421 agatgattgc cgccatcctt gacccacatc ggatagtcgt tggaatcgat acactttata
      481 caacgcagcg cgaaacattt acctcagaga gtcaacagct cattgaatca gcattgcaaa
      541 cacttcatgt gatgaattta aataacctgg aaacaaatct cgatgaggaa ggtcactatc
      601 tggcttttgg atataacgtt gacactcctg acagaataac cgtttctgct tataatgaaa
      661 aaaagcctgg ctgctatggc caatgtcttt taatacagga tgacgaaaca gacttattta
      721 acctgataaa agttaaaatt tttgaagcca ggcataatta tgtcgctcaa ttgatgagcg
      781 ttccggaatt tatgttcaaa tttagttata gcgctcaaca aaatagatta gaacctcatc
      841 ttctggttcc cacttatttc cctctaaaaa caaacgaacc ggttacgcaa gaggatgtac
      901 tgttacttta tcgtttcttt aaaatggatg gtgattttaa tatattaaca tcagatgaat
      961 acatgagtat catgacacct ctaatgaagt gtgagcgtag catccacgac cacaacaggt
     1021 atgtgacggg caaagatacc cttcttctgg actatcctcc ttctggtaac cagatatatt
     1081 tccatatttt tcctgacgaa tcagcaacac tagtccttta tttttcaaac aatgacattg
     1141 aatgtttcgt ctttgaaaga gatattccga gccagtatcg ctttttcaaa atgtttacca
     1201 atctggcact ggttattgat cagttaaccg atgccaatca aatactttaa tttttatata
     1261 ttccctgtgg caaatattgc caaacaatgc ccggggatta atttttatta ctcacggaaa
     1321 gatttaaata atgatcccat ctcaaatctc gttcaacacc cttcctgtca atgcaacata
     1381 cgcttcagag acaacagttg acacacaaaa attctctgac atcctctact cagcggggtg
     1441 ttccctaaag gatctggctt atctgagcag atgtctggca tcaatacatc cgactatcgc
     1501 caaaaacatt tatgaaacag aaaatctcag cgatcaaaaa ttactgcata ttgattgccg
     1561 tagtacaaat gaaatcaaga taaatatctt ttttggtcaa caacgagaag gattaattga
     1621 aattaattct gacactgtca tttttccgat attgtcttca ttaattgact ccgccatttc
     1681 taaatttcaa caccaaatcc ccgttaacgg ttcagtaaat cctgacctat tgtatgagga
     1741 ttatactgat taaatccctc ctgtcgcgaa aatggcctgc gttattgcca gacgtaataa
     1801 aacttttgat taacgttact cgtactggta gcaccttcat gtacttttct tttttacgaa
     1861 aaatcaaaag agctaacaaa ggtcataatt cgcatgaatc attaattatt tgttattttc
     1921 aacattattt ttacattaag cagagatgaa ttgcagtgaa attcgactta acttttatga
     1981 aatgcattta ctcatggcta aagccacgtc acaaaaatgc aaattagaag ttgatcacaa
     2041 ttatctcaat acatcaaatc aagttaattt taaaaattta acattcatga cattagttga
     2101 cataacacac tgaattaaaa tgagaatttg aaaacacact ttaaacaatc atttcccgaa
     2161 agacattttg aaaatagatc aattgcaaca ttacggtcag ataaagacac tcctgtgata
     2221 ttaaacctgt gccgttatga gcggccataa atacgacgtg tgtaacttat tttactctct
     2281 gtcttacaaa ggattaccca tgaagaaagc aacacttgtg attggcgtta ttggcgcgga
     2341 ctgccatgca gtaggcaata aagttctgga ccgcgttttt agcaaccatg acttcagagt
     2401 tattaatctg ggcgtaatgg tcagccagga cgaatatatt gatgctgcca ttgaaactgg
     2461 tgctgacgcg attgtcgtct cctctatcta tgggcatggc gatatcgatt gtctgggcat
     2521 gcgcgaatgc tgcatcgagc gtgggctggg taacatcctg ctctatgtgg gcggtaacct
     2581 ggtggtcgga aaacatgact tcgctgacgt ggaaaccaag ttcaaagaga tgggatttga
     2641 tcgcgtcttt tccccaagtc atgacttaga agatgtttgt cagctgatgg cccacgatat
     2701 caaccaacgt cataacgttg atactcgcat tcttgaagag gcaatctgat gcaaatcgtc
     2761 tctgtcgata ttggttcgac gtggaccaaa gcagccctct tcacccggga gggggacgcg
     2821 ttaacgttgg ttaaccacgt tctgacccca accaccaccc atcatctggc gaaggggttt
     2881 ttctccagcc tggaccaggt gttaaacgta gatgatgccc tccccctgtt aaacagtggt
     2941 gaggttgcat tgaagtattc ctcctctgcc aaaggtgggc tggcggttgc cgcaatggga
     3001 ttggtgcctt ctatcaccct ggaaaccgcg aaagtaaccg ctcactctgc gggtgctaaa
     3061 attgcgcaat attacgccta caagctgaac cgccgtgata tccaggcact ggaagaaacg
     3121 cagcccgata ttctcttatt caccggcggc accgatggcg gagaagaaag ctacggactg
     3181 aataacgccc gtgtgttggc agaatccaaa ctcgactgcg ccattattta tgccggaaac
     3241 cgggatattc aggacgaggt gcaggagatc ctcgggcata aagacctgac catcgtcgac
     3301 aacgttctgc ccgatcttga ccatccgaac cctttagctg cacgccaggc tatctgcgac
     3361 atattcctta aacgcatcgt taaagggaaa gggctggatg tcattgttga taaaacaggc
     3421 gaagaaccaa tgccgacacc atggacggtc tttgaactgg ttaaggcaat cagtaacgtc
     3481 gatcacagct ggaaagagtt catgctgatc gacatgggcg gtgcaaccac cgatgtctat
     3541 tccgcctgtg ccaacacgct ctcgcccgac actgttttac atggcgtacc ggaacccttc
     3601 gtaaaacgga cggtggaagg cgatctggga atgcgcgtct cggcaatggt ggttggtgaa
     3661 agtgcgaaag agttagtcag tgtgaatttt gctaagcaac ccgcacagtt ggacgctttt
     3721 tatcattacc tccgtcatct agtcgctcat cccgactact tacccgcgaa tgcagaagaa
     3781 aaatatttcg ataccgtcct ggccggactg tgtgtgggtt atgccaccga aagacatgcg
     3841 ggcaccaaaa aagaggtctg cacctgcgtc ggcaatgtcg atttacagat gggacgtgac
     3901 ctgacaaccg tgcgtaaagt cgtgggttcc ggcggttggc tctcgcgtgc cagtcagttc
     3961 gatatgcatc actggttgaa ataccgcgag ttaaacgacg acggtaaacg cattctctta
     4021 ccgaccgaat ttgaatatta ccgcgattcc cgggggctat taccactgct ggcaaacgtc
     4081 gccagagtcg atccactggc cgccgcgcgc accagtattc aatgcttaac cctataacaa
     4141 ttaaggctgc aatcaatgga acttcgaaat aagaaattaa cccatgacga attcatgacc
     4201 gagcggcaac aggtactgaa aacctgggaa accggcaagg atgttgaaaa ctttgaagat
     4261 ggcgttaaat accaacaaac cattcctgag cataaacgct tctctcttgc gctgttgaaa
     4321 gcagacaagg aaggtaaaac ccttagccaa ccgcgcgcag gtgtggcgct gatggacgaa
     4381 cacatcgaac tactgaaaac actacaagaa gagtgcgatc tgctgccgag cactatcgat
     4441 gcatataccc gtctgaatcg gtacgaagaa gcggctttag ggattaaaaa atccatcgaa
     4501 gccggaacct ctaaactgaa cggtttgccg gtagttaacc atggtgtcgc agcttgccgt
     4561 cgcctcaccg aagccctgca aaaaccgcta cagatccgcc acggcacgcc agatgcgcgt
     4621 ctgctggcag aaatctcgat gtccagcggc tttaccagtt atgaaggcgg cggcatttcc
     4681 tacaacattc cgtatgccaa acgcgtaacg ctggaaaaat cgatccgcga ctggcagtac
     4741 tgcgaccgtt taatggggat gtatgaagaa cacgggatcc gcatcaaccg cgaaccgttt
     4801 ggcccactaa caggtacgct gatcccgccg tttatctcgc actctatcgc gataatcgaa
     4861 ggcttgctgg cgctggaaca gggtgtgaaa tctatcaccg ttggttatgg acaggtcggc
     4921 agcctgacgc aggatgttgc ggcgatccag tctctgcgtg aacttgcaca cgaatatttt
     4981 cagagctatg gctataccga ctatgaatta agtaccgtgt ttcaccagtg gatgggcggc
     5041 tttccggaag acgaatcgaa agcgttcgct attatctcct ggggcgcagc cgtcgcgggg
     5101 atgtctggtg ccaccaaagt catcaccaaa agtccgcacg aagcctgtgg aatcccgact
     5161 gccgctgcca acattcaggg gctgaaagcc tcccgccaga tgctcaacat ggtcaatgag
     5221 cagaaattcc cgccgtgccc tgcggttgaa ctggaaattg aactgattaa gagcgaggtt
     5281 cgcgcggtac tcaataaagt gttcgaactg ggtaatggtg atattgcgcg cggtacggtg
     5341 ctggcatttg aagcgggcgt actggatgtg ccgtttgcac cagccgcgtg taacgcgggc
     5401 aaaattcttc cggtccgtga caacaccggc gcgatacgcg tactggaagc tggagccgtt
     5461 ccattgccaa aagatattct cgacctccac cacgattacg ttgctgaacg cgcacgcttt
     5521 gaaggacgcc aaccgacatt ccagatggtt gtagatgaca tcaatgctgt atcccacagt
     5581 aaattaatag gaagaccata atgaaaataa aacaggccct gttcaccgct ggctactcct
     5641 cattctattt cgatgaccag caggcgataa aaaacggagc gggtcatgac ggcttttttt
     5701 ataccgggga gccggtaaca caggggttta acgccgttcg tcaggccggg gagtgcgtat
     5761 cggtacagtt gattctggaa aacggcgcgg tcgccgtagg tgactgtgct gccgtacagt
     5821 attccggggc aggtggtcgc gatccactgt tcctcgctga gcactttatt ccgtttctca
     5881 acgaccatat caagccatta ctggtaggcc gcgatgtgga tgctttcctg ccgaatgccc
     5941 gtttcttcga caaattgcgt attgacggca acttgctgca taccgccgtg cgctacggat
     6001 tatcacaggc gctgcttgat gctaccgcgc tggcaaccgg ccgtctgaaa actgaagtag
     6061 tttgcgatga atggcagttg ccacgcgtgg cggaatccat tccattattt ggtcagagcg
     6121 gtgacgatcg atatatcgcc gtcgataaga tgatccttaa gggcatcgac gtgctgcccc
     6181 atgcactgat taataacgtc gaagagaaac taggctttaa aggtgaaaaa ctgcgcgaat
     6241 atgtccgctg gctgtcggat cgtattctga gcaagcgcac cagcgcacgc taccacccta
     6301 ccctgcacat cgatgtatac ggcactatcg gtctgatctt cgatatggat ccgcttcgct
     6361 gtgcgcaata catcgccagc ctggaaaaag aagcgcaagg cctgccgctc tacattgaag
     6421 ggccggtcga tgccggtaac aagcccgatc aaattcgcct gctgaccgcg attaccaaag
     6481 agctgacgcg cctcggttcc ggcgtgaaaa ttgtggccga tgaatggtgt aacacctacc
     6541 aggatattgt tgattttact gatgccgcca gttgtcacat ggtgcaaatc aaaaccccgg
     6601 atctgggcag tattcacaac atcgtcgatg cggttcttta ctgcaatagc cacagcatgg
     6661 aagcgtacca gggcggcacc tgcaatgaaa ctgatgtcag tgcccgcacc tgtgtccacg
     6721 tcgcccttgc cgctcgccct atgcgtatgc tggtaaagcc agggatgggc tttgacgaag
     6781 gcctcgatat cgtcttcaac gaaatgaatc gtactatcgc gctgttgcag gctaaggatt
     6841 aacaaatggc tcgttctttt aaagtgttat cgccaacggc aatcctcggg tatggattcc
     6901 cggaagagag cttccgtaaa gcgatggcag aatcgcccga tctgatcgcc gttgatgcag
     6961 gctcttccga tccaggcccc cactacctcg gggcaggcaa accgtttacc gatcgcgcag
     7021 gggtcaagcg cgatctgcgc tatatgatca ccgcaggcgt gcaaaataat ataccggtgg
     7081 tgatcggaac ggctggcggt tctggtgccg caccacacct ggagtggtgc cgagaaataa
     7141 ttcatgagat tgcccaggaa gagcacctct cgttctcaat ggcgctgatc ccggcagacg
     7201 ttgacaaagc catcgtccac caggcgctgg ataacggcaa aatcacggcg ctggagtttg
     7261 tcccgccgtt aacccacgac gcgattgacg aaagtacgta tatcgttgcg caaatgggca
     7321 tcgaaccctt ccagcgggcg ctgaaagaag gcgcgcaagt ggtgctgggc gggcgcgctt
     7381 acgacccggc ctgctttgct gcgcttccga tcatgcaagg ctttgatgaa ggtctggcgc
     7441 tgcattgtgg gaaaatcctt gaatgcgcgg caatcgccgc aacgcccggc tcaggctctg
     7501 actgtgcgat gggcatcatt gatgacaacg gctttacgct gaagacattt aatccgaagc
     7561 gtaaatttac cgaaacgtca gcggctgcac acacactgta tgagaaatcc gatccctact
     7621 tcctgcctgg acctggcggc gtgttgaacc tgaaaggctg cacatttaaa gcggtcaatg
     7681 acagcgaagt gtacgtcagc ggttctaagc atgaagaaac gccgtatgcc ctgaaactgg
     7741 aaggtgcgcg acgagtgggc ttccgctgtc tgaccatcgc aggaacgcgc gacccgatca
     7801 tgatcgccgg gatcgataaa atcatcgatg aagtcaaaac cagcgtttcg cgtaacctgt
     7861 cgctcgacga tgacagcatt cgcatcaatt tccacctgta cggcaaaaac ggggtgatgg
     7921 gagaccatga accgatgcaa actgccgggc atgagctggg gattgtcctt gatgtagttg
     7981 caccgaccca ggaaattgcc aacagcgttt gctcgctggt gcgctctacc atgctgcact
     8041 acggctatga aaaccgcatc gctaccgcag gtaatctggc gttcccgttc tccccttctg
     8101 atatccaggg cgggccagta tacgaatttt ccatatatca cctgattgaa gccaacgacg
     8161 ctctgcgttt tgatttccat attgaacagg tgacgccaga aggagttcag gcatgaaaca
     8221 gtcaattgtc tcactggcgc aagtcattcg ttcgaaaaat gccggacctt acgaactggt
     8281 tttagatatt ttatttaaaa cgaaagaaaa ctatgagcga gttaaatcat cagggcaatt
     8341 aaccccagaa ctgatcgcgc gcttatatca tgttgaacct gactttattc atcgtattgt
     8401 ctggtttgat ccttctaacg cagtaaaaat cgtcatgcca cgagatatta tctccgggaa
     8461 tgtaggtgac aatgatgtct atggcgcaca gcaacatgca cctttattaa atatgagttt
     8521 cgatttgtaa aaatccgaac acttttagcc aactgacatc ccgtcactta ttgcagttca
     8581 acacttcagc tatttgtaag gttgtaatag caggtagatg cctgaatctc tattcgggca
     8641 tctacttctc ctgattctga tttatcgcgg tgcacctgat ggaggaaaaa atgaagaaaa
     8701 taagtttaac cacgatgatt cttttggcgc tggtacttgg aatgattatc ggcgtagtgc
     8761 tcaataacac tgcttcaccg gaaaccgcaa aactctatgc acaagaaata tcgatattca
     8821 cgacgatttt cttacgactg ataaaaatga ttatcgctcc gttagtggtc tctaccctgg
     8881 tggtaggtat agctaaaatg ggagatgcca aagcccttgg tcgtattttt tctaaaacac
     8941 tctttttatt tatttgcgcc tcattgctgt caattgcctt aggcttgata acagtaaatt
     9001 tcttcatgcc aggcacagga attaattttg ttgcacacgg agccgaaacc accggagtgg
     9061 tcgcgtcaga accctttacg ctaaaagtat ttatttcgca tgctttcccc accagcattg
     9121 tcgatgccat ggcgcacaat gaaattttgc aaatcgtggt gttctcaatt ttcctcggct
     9181 gtagcctgac ggcgattggc gagaaaggca gcgccatcgt tcacgcctta gattcgctgg
     9241 cacatgccat gttaaagctc actggctacg tcatgctctt cgctcccctg accgtattcg
     9301 ctgctatttc agcattgatt gctgaacgag gactggcagt tatggtgagc gccgggatct
     9361 ttatgggtga attttatttc accatgttat tactttgggt gctgcttatc ggtctggcca
     9421 tcgtttatgt tggcccctgc atcagacgcc tgacccgcgc cctttcggaa cccgccctgc
     9481 tggcatttac cacatccagt tctgaagcgg cttttccggg aacgcttgaa aaactggagc
     9541 aatttggcgt ttcccccaaa attgccagct ttgtcttacc cattggctac tcatttaatc
     9601 tcgttggatc aatggcctac tgctccttcg ccacagtttt catcgcccag gcctgcaata
     9661 tccatttatc catcggtgag caaatcacca tgctgttgat cctgatgttg acctcgaaag
     9721 gaatggctgg cgtaccacgc gcctcaatgg tggttatcgc cgccacgctc aaccagttca
     9781 atattccgga agcggggctg atcttgctga tgggcgttga tccgttcctt gatatggggc
     9841 gttcagcgac aaacgtcatg agcaacgcaa tgggcgctgc gatggtgagt cggtgggaag
     9901 gcgaacattt cggcgagggc tgtcggggta aagcattaaa acccaatgaa tcgaacgttg
     9961 ctctgccctg agggggatct taagcaatca gaagccaatg actacgcatt tttataagcc
    10021 ggatcatgaa gttagacggc aattaagtaa aactaacgtt taaggtgaaa agtgaaatat
    10081 gtccaaacca tttatctggc aagaaccgtt tctacaaaac aaagacggta cagaatatac
    10141 attaattagc gatcaacata ttacagtaac ggaattagac ggcgaagaag tgataaaaat
    10201 cgctcctgaa gcattaacgc tgcttgcccg gcaggcattt tatgaagcct cttttttctt
    10261 acgttccgct catttgcaac aggtcgccag cattcttaac gatccgcagg ccagtagtaa
    10321 tgataaatac gttgcgttgc agttattacg taatgctgaa gtctcagcaa aaggggtatt
    10381 acccaactgt caggataccg gcaccgcaac tattgttgcc agtaaagggc aacagatatg
    10441 gacgggaggt aacgacgctg aagccttaag caaaggcatt tacagtactt tccaggaaaa
    10501 taatctgcgc ttttcacaaa atgcgccgct ggatatgtat accgaagtca atacgcaaac
    10561 caatctcccg gcgcagattg atatcagcgc tgtcgctggt aatgagtacc attttctctg
    10621 tgttaataaa gggggcggct ccgccaataa agccgcactt tatcaggaaa ccaaatcctt
    10681 attgcaacca gaaaaactga ccgcattttt aatcgaaaaa atgaaatcgc tgggcaccgc
    10741 tgcctgcccg ccttaccaca tcgcctttgt cgtcggaggg ctttcagccg atcaaacctt
    10801 aaaagttgcc aagctggcct ctacgaaata ttacgacaat ttgcccacca gcggaaacga
    10861 gcaaggacaa gcattccgtg atattgagct ggaaaaagta ttgctcgagg ccagtcagca
    10921 atttggtatt ggtgcgcaat ttggaggtaa atatttcgct catgatattc gcgtcattcg
    10981 tctgccgcgt cacggtggct cctgcccgat agctatggcg ctctcctgtt ctgccgatcg
    11041 caatatcaaa gcaaaaatta acaagcacgg tatctggctg gaaaaactcg aacacaatcc
    11101 ggggcaatat attccagcct cactgcggga agaaaatcac gcgcaacatg tacaactgga
    11161 tctaaaccgt ccgttgcgtg acgtgatgca ggatctcgcc agattaccgg taggtacccg
    11221 cgtgtcgtta agtggtccca tcgtagtggc gcgcgatatc gcccacgcca aaataaaagc
    11281 gcggctggat agcggcgaac cgatgccgga ttacctgaag catcatattg tctattacgc
    11341 tggcccggcg aaaacaccgg agaacatggc ctgcggttca ctgggtccaa ccacgggcgg
    11401 tcgaatggac ggttatgtcg ataccttcca ggctgctggc ggcagtctgg taatgttatc
    11461 gaaaggtaac cgcagccagc aggtcaccga tgcctgtcac aaacatggtg gcttcaatct
    11521 gggtagtatt ggcggtgcag ctgcgttact ggcgcaagaa tatgtcaaaa gtctgcgttg
    11581 tctggaatac ccggagctgg gtatggaagc ggtgtggatg atggaagtgg aaaatctgcc
    11641 cgcattcatc ctggtagatg ataaaggcaa taatttcttt agtcagtttg agcagcaaca
    11701 ccgctgcgcc agttgccctg cgggacatta aggaaaatga atggaagcgc gaatttcaac
    11761 tgaacggcat gtgcaacgcc agcagaaatt gaaggaacag gtcgatacgc gcgtcgcggc
    11821 ggcgatagag aaaaaaggga ttttgattgt tttcaccggc aatggcaaag ggaaatccac
    11881 tgccgcgttt ggtactgtga cgcgcgcggt gggtcatggc aaaacggtgg gggttgcgca
    11941 gtacatcaag gggcaatggg acaacggcga atacaacctt ctccagccgc tcggggttga
    12001 gtttcatatt atgggaaccg gttttacctg ggagacacag aaccggcaag cggatatcga
    12061 cgctgcgaaa gaggtctggt cggaaagcaa acggatgctg gctgacaagc gttacgactt
    12121 agtggtgctt gatgagctga cctatatgct ggcttaccat tatctggata ccgaagaagt
    12181 gatcgcttct ctgcaaaacc gcccggcaca acaaagtgtc attgtcaccg gacgcggttg
    12241 ccacagtcag atattaaaaa tggcggatac ggtcagcgaa atccgccccg tcaaacatgc
    12301 ttttgataac ggcattcagg cgcagccggg tatcg
//
LOCUS       DAOTRK010000136        12131 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.137, whole genome shotgun sequence.
ACCESSION   DAOTRK010000136 DAOTRK010000000
VERSION     DAOTRK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.137"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..466)
                     /locus_tag="QIE41_003719"
     CDS             complement(<1..466)
                     /locus_tag="QIE41_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDS3532557.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATTEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKK"
     gene            complement(513..1232)
                     /gene="yehT"
                     /locus_tag="QIE41_003720"
     CDS             complement(513..1232)
                     /gene="yehT"
                     /locus_tag="QIE41_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDS3532558.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRLERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1229..2914)
                     /gene="btsS"
                     /locus_tag="QIE41_003721"
     CDS             complement(1229..2914)
                     /gene="btsS"
                     /locus_tag="QIE41_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDS3532559.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3136..3867
                     /gene="mlrA"
                     /locus_tag="QIE41_003722"
     CDS             3136..3867
                     /gene="mlrA"
                     /locus_tag="QIE41_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDS3532560.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            3927..4034
                     /locus_tag="QIE41_003723"
     CDS             3927..4034
                     /locus_tag="QIE41_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDS3532561.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(4015..4746)
                     /gene="yehW"
                     /locus_tag="QIE41_003724"
     CDS             complement(4015..4746)
                     /gene="yehW"
                     /locus_tag="QIE41_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDS3532562.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(4751..5677)
                     /gene="yehX"
                     /locus_tag="QIE41_003725"
     CDS             complement(4751..5677)
                     /gene="yehX"
                     /locus_tag="QIE41_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDS3532563.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(5670..6827)
                     /gene="yehY"
                     /locus_tag="QIE41_003726"
     CDS             complement(5670..6827)
                     /gene="yehY"
                     /locus_tag="QIE41_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDS3532564.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWMTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(6834..7751)
                     /gene="osmF"
                     /locus_tag="QIE41_003727"
     CDS             complement(6834..7751)
                     /gene="osmF"
                     /locus_tag="QIE41_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDS3532565.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7900..10197)
                     /gene="bglX"
                     /locus_tag="QIE41_003728"
     CDS             complement(7900..10197)
                     /gene="bglX"
                     /locus_tag="QIE41_003728"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDS3532566.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLEAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDATRHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10393..12108
                     /gene="dld"
                     /locus_tag="QIE41_003729"
     CDS             10393..12108
                     /gene="dld"
                     /locus_tag="QIE41_003729"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDS3532567.1"
                     /translation="MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
BASE COUNT         2975 a         3410 c         2991 g         2755 t
ORIGIN      
        1 ttttcttcac atgctggcgc gctgccagtc cacgcagaaa ataacgtaaa aattgatcgc
       61 cgcattcgcg gaagttttta tgatccggtg cacgcatcat cgccgtaatt tccggcatcg
      121 aaacgcggaa ctgctgttcg gtgaggatcg ccagaatgtc atcggtcttc agcgaaaacg
      181 cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg acgttccggc tccagtgccg
      241 gagcagactc atccttgccg cgtttttcat aaatcaggcc attgaggaag gaagacaaaa
      301 caatgtccgg acaacgctga aaaccctctt cgtcttcttt acgtagccag acggcgatct
      361 gttccgtggt ggcttcgaca ttacccagcg ccagaatacg caccaggtca ttattattgg
      421 ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact tagcatgagg ccttcaggtg
      481 ttgatgaggc aaaaagccat tttagcagtc ttttacaggc caatcgcctc ttttaagctt
      541 ttcagataac gacggctgac cggcacggtt aagccattac gcaaaatcaa ctcggcctgg
      601 ccgttatctt ccagacgaat ctcctgtaaa tgcgcgaggt taaccagata ctgacgatgg
      661 cagcgtagta gtggtgtacg actctccagg gtacgtaatg tcaattcggt aaagccctct
      721 ttcccttcgt gactggtaac gtagacaccg ctcatccgac tgctgacaaa tgctacatct
      781 ttcatttgca gcaaataaat ccgactatgc cccgtacaag ggataaattt cagcgcctgt
      841 tgattttccg gtaacagcga aacatcctgc ttgctgcgct ccagacgcaa tcgcgccagc
      901 gttttctcca gtcgcgcttc atcaattggc ttcagcagat aatcaaaagc atgttcttca
      961 aaggctttaa tggcgtattc atcaaacgcg gtgagaaaaa cgatatacgg acgatgttcc
     1021 gggtcgagca tccccaccat ttccagacca ctgatgcgcg gcatctggat atcaagaaac
     1081 agcacatccg ggcgcagttt atgcaccgcg ccgatccctt ccacggcgtt tgaacactct
     1141 ccaacgattt caatatcgct ctgctcctgc aaaaatacgc gcaggttctc ccgtgctaac
     1201 ggctcatcat cgacaattaa gactttaatc atgcctcgtc cctccatggt agtcgtaacg
     1261 ttattcgggt gtaactatca ggctcacagg cgacgcttat cccatagtca tcgccaaacc
     1321 gttcacgtaa acgcttatcc accagattca tccccagccc actggcattg gttaccggtt
     1381 gatacaaacc ggcgttgtct tcgatctcca gcatcaaatg ttgcccctca cgtcgggcgc
     1441 tgattgccac tcgccctgta tccagcagtt gtgatgtccc atgtttaata gcgttttcca
     1501 ctatcggttg cagggtaaac gcgggcaatt gctgctggga taattcttgc ggaatagcaa
     1561 tgttgacctg caaccgcgac tggaagcgcg ccttttcaat ttgcagataa gcgttcacat
     1621 gttcaatttc gtcggcgaga gtaacaaact ccgaaggccg ctttaagttt ttgcggaaaa
     1681 aagtggaaag atactgcacc agctggctgg cctgttcgct gtcgcggcgg atcaccgctt
     1741 taatggtgtt aagcgcatta aacaaaaaat gggggttcac ctgggcgtga agcagtttga
     1801 tctctgactg ggtgagcatc gctttttgcc gctcatattg cccggcgagg atctgcgccg
     1861 aaagcagttg cgcaatcccc tcacccagcg tgcgattgat tgaactgaat aaacggtttt
     1921 tggcttcata caatttgatg gtacccatca cccgctgatt ttcaccacgc aacggaatta
     1981 ccagcgtcga ccccagtttg cattgcggat gcaaagagca acgataaggt acttcgttgc
     2041 catcagcgta gaccacttca ccggtttcaa tcgcttttaa ggtgtaagtc gaagaaatcg
     2101 gtttgccggg taaatggtga tcgtcaccaa ttccggtaaa ggccagcaat ttctctcgat
     2161 cggtaatcgc gactgcacca atatccagct cctgatacag cacctgtgcc actttcatgc
     2221 tgttcacttc gttaaacccc tggcgcaaaa tgccttccgt cgaggcagcc acttttagcg
     2281 cagtggcaga aaaagccgaa gtgtattttt caaacatcgc gcgtttatcg agcaatatac
     2341 gcataaacag cgccgcgccg acggtattgg tgaccatcat tggcgcagca atattactca
     2401 ccagacgcac cgcatcttca taaggtcggg cgattgccag gatgatcaac atttgcacca
     2461 tttcagcgac gaacgtgacg gcaccggcgg taatggggtt aaagacttta tcagtgcgcc
     2521 cgcgacggat caggatgctg tgtaccaggc cgccgagcaa cccttcaacg atggtcgaga
     2581 tcatgcaact tagcgcggtc atgcccccca tcgaatatcg atgtaacccg ccggtcagac
     2641 caaccagccc accgacgacc ggaccgccga gtaagccgcc cattaccgcg cctatcgcac
     2701 gggtattggc aatagaatcg tcaatgtgca acccaaacca ggtgcccatg atgcagaaga
     2761 tggaaaagac gatgtagcag agaaatttat gcggcagacg aaccgtgacc tgcattaacg
     2821 gtatgaataa tggcgtttta ctcattaacc acgcaatgac taaaaaaacg cacatctgct
     2881 gaagcagcag caacaccaga ttaaaatcgt acatacccgc aaaccacact tccctttaaa
     2941 acgcgtaaca tacattgcct gcgtttaact ttctttgaac tcttgcagaa aaatgagaat
     3001 tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa atcaccctat agatgcacaa
     3061 aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa agttaaaccg ggacctcgcg
     3121 agcaagggtg agacgatggc gctttacaca attggtgaag tggcgttgct ttgtgatatt
     3181 aaccctgtca cgttacgcgc gtggcagagg cgttacggat tgctgaaacc gcaacggaca
     3241 gacggcggcc atcggctgtt caacgatgcc gatattgacc ggatccgcga gatcaaacgc
     3301 tggatcgaca acggcgtgca ggtcagcaaa gttaaaatgc tgctcagtaa tgaaaatgtt
     3361 gatgtgcaga acggctggcg cgatcagcaa gaaacattac tgacttacct gcaaagcggc
     3421 aatctgcata gcctgcgaac gtggatcaaa gagcgcggtc aggattaccc cgcccagacg
     3481 ctcaccacac atctgtttat tcctctgcgc cgacgacttc agtgccaaca accgactctc
     3541 caggcgctgc tggcgatcct cgacggcgta ctgatcaact acatcgccat ttgcctggct
     3601 tcggcacgta aaaaacaggg taaagatgcg ttggtggttg gctggaatat tcatgatacc
     3661 acccgtctgt ggctggaggg ctggattgcc agtcaacaag gatggcgcat tgatgtcctc
     3721 gcccactcgc tcaatcaact acgccctgaa ctgttcgaag gccgtacatt gctggtgtgg
     3781 tgcggtgaaa atcgaacctc cgcccaacag cagcaactca ccagttggca ggaacaaggc
     3841 catgatattt tcccactcgg catttaatga ttcgttaaca aatgcgcttt actgtacaat
     3901 cctttcgtta acataaggag tgcattatgc gcatagctaa aattggggtc atcgccctgt
     3961 tcctgtttat ggcgttaggc ggaattggtg gcgtcatgct cgcaggttat acctttattt
     4021 tgcgtgctgg ctaagcgcct gcaccagcct ttcaaacagg cggtctgcaa tgatcgccgc
     4081 cagtgccacc agtaacgccc cctggatcac atacgcggta ttaaatccgc taagcccgat
     4141 gatgatcggc gtacccagcg tgctggcccc taccgttgag gcgatcgtcg ccgtaccaat
     4201 gttgataatc accgaagttc gcacgcccgc cagaatcacc ggagccgcca gcggtagctc
     4261 gaccttacgc agtcgctgac cacggctcat tcccatacct ttcgcaactt ctgtcacgct
     4321 ggcatcaatc gctcccagcc cggcaagtgt tgcctgcagg acgggcagca caccataaag
     4381 gatcaaggcg ataatcgcgg gttgcagacc aaacccaatc accggaacgg caatcgccag
     4441 caccgcgacg ggcggaaaag tctgtccaac ggcggcaata gtttccacca gtgggcgaaa
     4501 ttccgcgccc cacgggcgag taacaataat tcctgcacca gtgccaatga tcaccgcaaa
     4561 caaactcgaa attcccacca gccagaaatg agccagtgcc agagcagcaa aactttcttg
     4621 ctgataaacg ggtcgtggca gttgtgggaa caaggcagca aacagcggct ggctgtaagg
     4681 cagccagaaa atcagcgcca caaacagagc aatgagccag aacagcggat cgcgcaacat
     4741 cttcatacgt ttacgcctcc accagcagat cctgaaaatg cagcgtgccg caaggctggc
     4801 cctgcgtgtt caccaccggc agcacctcgc atccccgcgc gacaaacagg gagagcgcat
     4861 cgcgtagagt catctcttct gccagcgcct caccttctgc ccgttcttcg cgacgcacgt
     4921 aatccgccac gctacgtaac gaaagcaggc ggacacccag ttcactatgc ccaaaaaact
     4981 ggcggacaaa atcattcgcc ggacgagtca gcatcgtcag cggattgccc tgctgcacca
     5041 cttcaccgtg atccatcaac accagatgtt ctgccagtcg tagcgcctca tcaatatcat
     5101 gagtgaccag cacaatggta cgccccagca aacggtgtat gcgcgtcatc tcttgttgca
     5161 acgcgccgcg cgttaccggg tccagcgcgc caaaaggttc atccatcagt aagacttgcg
     5221 gatcggcagc cagtgcgcgc gccactccca cacgttgctg ctgaccgcca gaaagctgat
     5281 gcggataacg ctcacgcaaa tttggctcca gccccagtag cgccattaat tcgtcgatac
     5341 gatcgtcaat ccgcgcccgc gaccattttt gtaattgcgg cacggtggca atattttgcg
     5401 ccacgctcca gtggggaaac aggccaatag attgaatggc atagcccatc cggcggcgca
     5461 actccagcac tggcagcgag cgaatttctt ctccggcaaa gcggatcacg ccgctgtcat
     5521 gctccaccag gcggttaatc attttcaggg tggtggattt accggagcct gatgtaccaa
     5581 tcagcaccga aaaactcccc tcctgaaaat tgagattgag atcgttaacg gctttttgtg
     5641 cgccgaacag tttgctgaca tggctaaatt caatcattac gtttcacctt cagcagtgcg
     5701 ataagcaaat cgaacagcgc atcgatcagc accgccagaa caattaccgg gatcaccccc
     5761 agcaacacta aatcaatggc actgcttagc agcccctgga aaaccagcgc accaaaaccg
     5821 cctgcgccga ttaacgccgc aatcaccgcc atgccgacgg tttgcaccat caccacccgc
     5881 aggctacgca gaaataccgg taacgccagc ggtaactgaa catgcaggaa tcgctgcgcc
     5941 ccgctcatgc ccatcgctct ggcgctctcc agcacatcgc gcgggatctg gttcaagccg
     6001 actaccacgc cgcgcaccag cggcagcaag gcatagagca ccagcgcaat cagtgcgggt
     6061 gtcattccgg ttcctgctat gccgagctta cccagccacg gaaaggccgt aaccagtccg
     6121 gcaagcggcg caatcaacaa gccaaagagc gccaccgaag gcacggtctg aatgacattg
     6181 agtagcgaaa aaatcgcccc ctgccgcgca gtggaaaagt agcaccagac gcccaacggc
     6241 acaccaatca ctaacgcagg cagcaccgca ccaaacagca acgtcagatg ttgtgccagc
     6301 gcgtcgtcaa acacatcctg acggttggcg tattctttca ttagcgagag atcgttaagc
     6361 gtgccggagt acagcaacca cagcggaata atggcaatct gcatatgcaa caaccagcgc
     6421 cacagcggat gcgtggagat tcggcggatg gcatcgctac aggccagcaa tgccagcgcc
     6481 gcagccagcc agaaaccact gccgaggctg gtacgcgcca gcgcactgcc attttgtgcc
     6541 agttgtgtcg ccgcctttcc tgctccccac accagcaata cgaagacgaa ttgcgccaga
     6601 atgagtgcaa aaatgctgcc ttttttagcg ggaataaaac aggctgtcat ccaggcgcaa
     6661 ccaacgccca ccagcatcca gatcgtttgc ggccacagct gccagagatg acgcccctca
     6721 cccgaaacta aacgattagg cgcgtaactg ataaatggca gcgccgctgc gattgccgtc
     6781 agcaacagca gcagcgccag aacaggatta atacggaaat aagtcacggg aaattacttc
     6841 gtccaccctt tttgtttcag gtagtcggca gccacttttt tggcatccag tccttccaca
     6901 gcaatgctgg cattcagttg ctgcaatgtt ttttcatcga ggctggcgaa gactggctgt
     6961 agccactgtg ccatttgtgg atactctttc aacaccgact cacgcaccac tggtgcaggc
     7021 gcgtagatag gttgcacgcc ttgcggatca cttaaggttt gcagccccag cgccgcgacc
     7081 gggccgtcag tgccgtaagc catcgccgca ttaacgccag aggtttgctg ggcagcggct
     7141 ttgatcgtca ccgccgtgtc gccaccagcc agtgacagca actgatcctg accgagctta
     7201 aagccatagg ctttttcaaa cgcgggtaac gcatcggcgc gttcaataaa ttctgccgag
     7261 gccgccagtt tgaaggtgcc gccctctttc agataacgac tcaggtcggc aagcgaagtg
     7321 agtttgtttt tctctgccac atcctgacgc acggcgatag tccaggtgtt atttgcaggc
     7381 gcgggcgtca gccagattaa cttgttttgc tctgcatcga gttttttgac tttctcgtag
     7441 ccttgcccgg catttttcca tgccgcatcg ttttcatctt taaagaaaaa cgcgccattg
     7501 ccggtatatt ccggatagat atccagttca ccggaagtaa tcgccccgcg caccacggga
     7561 gtcgttccca gttgcacttt atttaccgta ggaacaccgt gactttccag cacctgcaaa
     7621 atgatattgc cgagcagcgc gccttcggta tcgatttttg aaccgacctt aacgggggaa
     7681 gccgcctgta gcggcaggct cacggctgcc aacaaaacca atgaacctgc ccagagcttt
     7741 gagagtggca tgatgctttc ctcattcttt tactgttgtt ttcagcgaat taagagaaaa
     7801 gcatagttga taatgacagg gttagcctgt ctgggcggat tcagttgcag aatcagataa
     7861 atacttaaag ggcgcagacg tcgcgccctt actaaagcat tacagcaact caaactcgcc
     7921 tttcttaacg cgtgcggaat cagtgccgat aaagacattg aacttgccag gctcggcgtc
     7981 atatttcatc tgttgattcc agaacttcag cgcctcgata tcaatcggga agctgacggt
     8041 ctgggtttcg cccggtttca gggtgatttt ctcaaagcct ttcagctgct tcaccggacg
     8101 actcatggaa gccgtcacat cctgcaagta catctgcact accgtcgccc cttcgcgctt
     8161 accggtgttc gtcacctgca cactggcggt cactttgccg tcacgcttca tggtcggcgc
     8221 agaaagtttc acatcagaga cggtgaaagt ggtatagctc agaccatagc cgaacggata
     8281 aagcgccccg ttagcttcat caaaataacg cgaagtgtat ttgttcggct tatcggcgtt
     8341 atacgggcga ccggtattca gatggctgta gtacacaggg atctgcccga cagaacgcgg
     8401 gaaggacatc ggcagcttgc cggacgggtt gtaatcgcca aacaatacat cggcaatcgc
     8461 attcccccct tcagtccccg caaaccaggt ttccagaatc gcatcagcct gctgatcttc
     8521 tttcaccagc gccagcggac gcccattcat cagcaccagc accagcggtt taccggtggc
     8581 tttcagcgcc gcaatcaagt cacgttggct ttgcggaata gtgatatcgg tacggctgga
     8641 agcctcgtgc gccatcccct gtgcttcacc gaccacagcc accaccacat cagattgttt
     8701 cgcggtttgc accgcttcat caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc
     8761 ttcttcatac tgattcagga aatcgataat gcctttgtca ctggtaacgt tagccccttt
     8821 ggcatacagc actttaccgt tttcaccgac ggcattttta atcccggtca gcacggtcac
     8881 ggattgatcg gcaacccctg ccgccgacca gctgcccatc acgtcacgct tgctgtctgc
     8941 cagcgggcca accaccgcaa tggttgcgga ttttttcagc ggtaacgttt cgagacggtt
     9001 tttcagcaac accaggcttt cgcgcgccac ttcacgcgct tctttgcggt gcaggcggct
     9061 ttcggcattg gtatccaccg ggtcagactc tttcggaccg agatggctgt acgggtcgtt
     9121 aaacaacccc atatcatatt taacgttcag tacatgacgg gtagcgtcat ccagctcttc
     9181 catcgtcact ttgccggatt tgatcaaccc aggcagatac ttcgagtagt actcgtcgct
     9241 catactcatg ttgatgccgg atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc
     9301 cgtgccatgt ttaatcagct ctttgattgc gccgtgatcg gaaacggtga tgcctttaaa
     9361 gccccactgg tcgcgcagaa catctttcag cagccaggag tcggaggtgg ctggcgtacc
     9421 gttcagcgag ttcagcgcca ccatcaccgc gccgctgcct gcctccagcc ccgctttgta
     9481 cggcggcata taatcattaa acaggcgctg cggactcata tcgacggtgt tgtactcttt
     9541 accgccttct accgcgccgt aagcggcaaa gtgtttgacg ctggtcatca ccgagtagcg
     9601 atctgccgga cttttaccct gcatcgcttc caccatggtt ttccccatta ttgaggtgag
     9661 atacgtatct tcgccaaaac cttcggaagc acgtccccag cgcggatcgc gcgagacatc
     9721 gaccattggt gcccaggtca tattcaggcc atcatctgcc gcttcataag cagagacacg
     9781 cccgactgtt ttcaccgcat cgaggttaaa agacgaggcc agaccgaggc taatcgggaa
     9841 caccgtgcgc tgaccgtgca gcacgtcgta agcaaagaaa agaggaattt tcaggcggct
     9901 taattccatc acctgatcct gcatggcgcg gatatcctga cgggttacgg tgttgaaaat
     9961 cgccccaacc tgcccgtctt tgatcatctc gcggatcgcc tctttcgggt tatccgggcc
    10021 gacgctgatt aagcgcagct gaccaatttt ctcatcaact gtcattttct taagcagttc
    10081 ggtgacgaac gcatcccgcg cttcaggcgt taatggatgg ttaccgaata aatcatccgc
    10141 cagtgctggc tgcagagcca gactcaccgc gattcctact gaacatagcc atttcatatg
    10201 gatttttctt cctttgttgc ccgacgtggc agcgaaaatg gtgcaaaaac cgtagtttgc
    10261 cataagcatg atggagagag aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat
    10321 tcactcgctg aattgtcata caaggcgcta ttctagtttg tgatattttt tcgccaccac
    10381 aaggagtgga aaatgtcttc catgacaaca actgataata aagccttttt gaatgaactt
    10441 gcccgactgg tgggttcttc acacctgctc accgatcccg caaaaacggc ccgctatcgc
    10501 aagggcttcc gttctggtca gggcgatgca ctggctgtcg ttttccctgg ctcactactg
    10561 gaattgtggc gggtgctgaa agcctgcgtc accgccgaca aaattattct gatgcaggca
    10621 gccaatacag gtctgaccga aggctcgacg ccaaacggta acgactatga tcgcgatatc
    10681 gtgatcatca gcaccctgcg tctcgacaag ctgcacgttc ttggcaaggg cgaacaggtg
    10741 ctggcctatc cgggcaccac gctctattcg ctggaaaaag ccctcaaacc gctgggacgc
    10801 gaaccgcact cggtgattgg atcatcgtgt ataggcgcat cggtcatcgg cggtatctgt
    10861 aataactctg gtggttcgct ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc
    10921 gcgcgtataa atgaagatgg caagctgacg ctggttaacc atctggggat tgatctgggc
    10981 gaaacgccgg agcagatcct tagcaagctg gatgacgatc gcatcaaaga tgacgatgtg
    11041 cgtcacgatg gtcgtcacgc ccacgattat gactatgtcc accgcgttcg tgatattgaa
    11101 gccgacacgc ctgcacgtta taacgccgat ccggatcggt tatttgaatc ttctggttgc
    11161 gccgggaagc tggccgtctt tgcggtacgt cttgatacct tcgaagcgga aaaaaatcag
    11221 caggtgtttt atatcggcac caaccagccg gaagtactga ccgaaatccg ccgtcatatt
    11281 ctggctaatt tcgaaaatct gccggttgcc ggggaatata tgcaccggga tatctacgat
    11341 attgcggaaa aatacggcaa agacaccttc ctgatgattg ataagttagg caccgacaag
    11401 atgccgttct tctttaatct caaggggcgc accgatgcga tgctggagaa agtgaaattc
    11461 ttccgtccgc attttaccga ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat
    11521 ttaccgccgc gcatgaaaaa ctggcgcgat aaatacgagc atcatctgct gttaaaaatg
    11581 gcgggcgatg gcgtcggtga agccaaatcg tggctggtgg attatttcaa acaggccgaa
    11641 ggcgatttct ttgtctgtac gccggaggaa ggtagcaaag cgtttttaca ccgtttcgcc
    11701 gctgcgggcg cagcaattcg ttatcaggcg gtgcattccg atgaagtcga agacattctg
    11761 gcgctggata tcgctctgcg gcgtaacgac accgagtggt atgagcattt accgccggag
    11821 atcgacagcc agctggtgca caagctctat tacggccatt ttatgtgcta tgtcttccat
    11881 caggattaca tcgttaaaaa aggcgtggat gtgcatgcgt tgaaagaaca gatgctggag
    11941 ctgctacagc agcgcggcgc gcaataccct gccgaacata acgtcggtca tttgtataaa
    12001 gcaccggaga cgttgcagaa gttttatcgc gagaacgatc cgaccaacag tatgaatccg
    12061 gggatcggta aaaccagtaa gcggaaaaac tggcaggaag tggagtaaaa attacggatg
    12121 gcagagtatc g
//
LOCUS       DAOTRK010000137        12065 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.138, whole genome shotgun sequence.
ACCESSION   DAOTRK010000137 DAOTRK010000000
VERSION     DAOTRK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.138"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1..359
                     /gene="cbtA"
                     /locus_tag="QIE41_003730"
                     /pseudo
     CDS             1..359
                     /gene="cbtA"
                     /locus_tag="QIE41_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            356..550
                     /locus_tag="QIE41_003731"
     CDS             356..550
                     /locus_tag="QIE41_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532568.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            563..676
                     /locus_tag="QIE41_003732"
     CDS             563..676
                     /locus_tag="QIE41_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDS3532569.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(965..1111)
                     /locus_tag="QIE41_003733"
                     /pseudo
     CDS             complement(965..1111)
                     /locus_tag="QIE41_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1165..1347
                     /locus_tag="QIE41_003734"
     CDS             1165..1347
                     /locus_tag="QIE41_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDS3532570.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(1448..1777)
                     /locus_tag="QIE41_003735"
     CDS             complement(1448..1777)
                     /locus_tag="QIE41_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDS3532571.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1949..3007)
                     /locus_tag="QIE41_003736"
     CDS             complement(1949..3007)
                     /locus_tag="QIE41_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDS3532572.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRLLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3205..3678)
                     /gene="sbmC"
                     /locus_tag="QIE41_003737"
     CDS             complement(3205..3678)
                     /gene="sbmC"
                     /locus_tag="QIE41_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDS3532573.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(3797..4963)
                     /gene="dacD"
                     /locus_tag="QIE41_003738"
     CDS             complement(3797..4963)
                     /gene="dacD"
                     /locus_tag="QIE41_003738"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDS3532574.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5172..6599
                     /gene="sbcB"
                     /locus_tag="QIE41_003739"
     CDS             5172..6599
                     /gene="sbcB"
                     /locus_tag="QIE41_003739"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDS3532575.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDAERLGIARQHCLDNL
                     KVLRENPQVRDKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDDNEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQLYADDKEKVALLKALWQYAEEIV"
     gene            complement(6642..6869)
                     /locus_tag="QIE41_003740"
     CDS             complement(6642..6869)
                     /locus_tag="QIE41_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDS3532576.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(6883..7941)
                     /gene="yeeE"
                     /locus_tag="QIE41_003741"
     CDS             complement(6883..7941)
                     /gene="yeeE"
                     /locus_tag="QIE41_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDS3532577.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYVAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGSFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(8120..9478)
                     /gene="plaP"
                     /locus_tag="QIE41_003742"
     CDS             complement(8120..9478)
                     /gene="plaP"
                     /locus_tag="QIE41_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDS3532578.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(9468..9530)
                     /gene="yoeI"
                     /locus_tag="QIE41_003743"
     CDS             complement(9468..9530)
                     /gene="yoeI"
                     /locus_tag="QIE41_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDS3532579.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(9745..10674)
                     /locus_tag="QIE41_003744"
     CDS             complement(9745..10674)
                     /locus_tag="QIE41_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3532580.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(10720..11544)
                     /locus_tag="QIE41_003745"
     CDS             complement(10720..11544)
                     /locus_tag="QIE41_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS3532581.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            11971..12021
                     /gene="hisL"
                     /locus_tag="QIE41_003746"
     CDS             11971..12021
                     /gene="hisL"
                     /locus_tag="QIE41_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDS3532582.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    11997..>12065
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
BASE COUNT         3271 a         3133 c         2787 g         2874 t
ORIGIN      
        1 gtattacccg ggcaggcggc cagttctcgc ccgtctcctg ttgaaatctg gcagatactg
       61 ctgtcccgac tgctggacca gcactatggc ctcacacaga atgacacacc ttttgccgat
      121 gaacgtgtga ttgagcagca tattgaggca ggcatttcac tgtgtgatgc ggtgaacttt
      181 ctcgtggaaa aatacgcgct ggtgctaccg accagccggg attcagcgcc tgtacccgct
      241 ctcagttaat aaacagcatc gatatcctcc gggctcgcag ggcgaccggc ctgatgaccc
      301 gcgacaatta cagaacggta aataacatta ccctgggtaa gtatccggag gcgaaatgat
      361 gacactggaa gccgacagcg ttaacgtaca ggcgctggat atggggcaca ttgtcgttga
      421 cattgatggt gttaatatca ctgaactgat taataaggcc gctgaaaacg gttattcact
      481 ccgcgtggtg gatgaccgtg actctaccga aacaccggca acttatgcca gccctcacca
      541 gttgctgtaa gacaatgcag tgatgcaggg tatagtgcca ttaaccacag aaacagcact
      601 gttgtcctgt attaagcagg caagagaaga tgtccttacc ctgcgccatc tgcagcttct
      661 gcaccagaac cggtgaatct cactcaacag gcttcatttc ccctgacgcc agcctgaata
      721 cagctggcgt tttcatttat aaacagaaag gaaaaccgat aataatggaa tggacgcccc
      781 gaccatgaag caacggtagg atattactga taaaccttcc agtggaggtg cgttatggcc
      841 gttttaacga ttagcattga cctgacaaaa aatgaattcc aaatccacgg tctgggtagg
      901 aacagaaaaa tctaagctca gaaaacggat taaagtaagc gtcaacggag caccatattg
      961 acgcttattt attggtgaga actacgttcc atggcaggag ttcgtcaaca cggttggagg
     1021 gccattccgg cagtatgctc agaatatggc gcagatacgc ttccggatcg ataccgttca
     1081 gacggcaggt gcctgctttt ccggcaacaa gcaggaatct cagcgctgaa gcaggaatta
     1141 gaggtaaaaa cgccctaccg ggccatgaat cacccggtca ttggagtagt aaccaaagca
     1201 gatttagcca gcatggaaca aatatctttg gtgaaatgtt ggttacggga agctggagcg
     1261 cacaacgtgt tagtaaccag tgcggttaat aacaatggag ttacagaact ttttgccttg
     1321 ctgcatacag aagaaggctg ccgctaattt cgataggttc tcagatgaga atgatgacgg
     1381 tgatacacag ataagaatca atgagcaatg agggttgccg ggcaaccctc attgaataaa
     1441 acaggaatta cttcgcttcg ccgtttttca tttcgcccat agctttcagc tttttggaga
     1501 tatcgcggcg ttctttggag agctcggcat ttttgatgat gtaatcgtca acgcgatctt
     1561 catagtcacc tttcatgctg gcgatgatgc cctggattgc ttcaacgctc atccctggct
     1621 tgatgtaatc gctcaggttg tccagcaaca ggacgcgctt ctggttgtca cggatctttt
     1681 tctcaacgtc ctgaatttca cgttgcagtt tgttcttacg acggaacaga cgaacaaatt
     1741 ccagtacgtc ctggaatgaa ggcttggtag tttccatttt tatacccctg ataatgtgag
     1801 agtcggattc gtttaagcaa ccgctattcg atagggccaa cattactcat agctgccgca
     1861 gatgacaatg cttttatccc ttttcactat catacccttt atccttgctg aatcgaagca
     1921 gcagcaagat gattctgaag ttcaggaatt atttgcgcaa ggcccggcaa atcagattcg
     1981 atagcaactc aagctgatgc gccgtttcca tgttcagcca cacataacca tagattggtg
     2041 tttccacaac cttcaggtca tggtggttat taagcaacag acgcagctct tccacagcat
     2101 cgttcaattt ttccgtattg gcaaaaaccg gctgcggatt accttcgtac agcgcatgaa
     2161 caaggctcag cagtatttgc tgcatcatgt gctgggtatc acgaagtttt tgcgcgttca
     2221 ataacacgaa atggctgggg cgcgtggccc agtatgcatt gatttgcaac tccagcatac
     2281 aaaccagatt gcggttaatg gtctggatac cttcatatat cgattttgga atacgggttt
     2341 ctttgctggc gggcgcaatc agcccacgca ttttcacggc atcggtcagc agtttttgta
     2401 gatggctttc cagacgtggg cgttcgagta agttcggtga gaatgcagat tgatagaccc
     2461 gattatactc ggtcagactt ttcgccagtt gaatgcgcca gtggatgaac gcccgttgtg
     2521 gccagatacc ggtaaacaac attgccagta aagagccgag gatcacatcg ccgcttcgcc
     2581 ataacgccgt atcaatttca cctgtcgggg aacccacaac aattgccagc gtcaccccaa
     2641 tcaataatcc ttgatacggt ttcttgccca gcgccagcca accgcaaaga aacatcgccg
     2701 ccgcgcacca gactaacatc agcggtaacg aaattaactc cagttgcaga gcgataagac
     2761 ctaaaatcga acccaacacc gtaccgccaa tacgttcgaa ggcacgaggg acaacgttcc
     2821 cccagaacga gattggcccc ataatcacca ccatggtgac cagcggccag gtgctttccg
     2881 ggatagtaaa caggcggata atgagaaaag tgagcaggaa tgccagcgcg acccgagtac
     2941 catgcacaat gcggtaatgg cggtataccc ggatttcaaa cgggcttaat gacttatcgg
     3001 cacgcacgca gaaactccgt aatgaagatt aaaagcgctc ataacacatc tgtcggccag
     3061 aaggcgcgcc tctgagttat ccgacggaca taccgggaac agtgtaattt cccggcagga
     3121 gcgaaaaatt tgaaaggcgc atcttactct tttcgctttc aaaaaaagag tggtcttcgc
     3181 gtcaacacac cgccctgaga tgaattagtg atgttttggc tgcaccgcaa catacatttc
     3241 gatatcccag tacccatctt ccgcgccatt gttcaagtaa acctcgaagc agggctttgg
     3301 taacatttca taggcactgt cctgcaagag gctattaaag aactgatacc agggtttagc
     3361 aaaatcatca ccgactacac gagccaccgc caccgcatac tgaccacctg aaatttctgt
     3421 cagaatgacg ccctcactgt tttcggggag cgtaaagtta ttcggcaccg tcacgacggt
     3481 gtcgcagcgt aatttttcgg cgggtgtttc atctggattg tcgtaataga cagcaaccca
     3541 ctccttcggc acaatatttt tgctatctat ccacatcatc aactgctcaa agcctttctt
     3601 taccgtctgt tcccacgggc caacgagatg gaaacctgca acggtacgtt tatcttcctg
     3661 cttaatctcg tagttcatga cgcccccatt aatactgttt ttatatacag tatagttgca
     3721 atattaaaac cacaaggaat gagtgttgat tatgcgagca gactcgcact cctgccagtc
     3781 tgctgcaaaa gaaaggtcag gccttatggt ggaaataatc actcagccgg gaaaacatgc
     3841 tgccttcccc gacagattcc agggtaacca gcggccagtg cgccacctgt ttatcacggt
     3901 cgtaaagttc aatttcccct acccgctgat gggcgctaat tggcgcggtg agttctttac
     3961 catcaagggt atatttggct ttgatatgtg gaatttcggc tttcggtagc accatccaga
     4021 actcttgttc cgttcccagg gcgatatttt ctttatcgcc ataccagatg cgttccgtac
     4081 caaccttttt cccacggtgc aaaatttgca ccgtagtaaa gttttgttga ccccaacgca
     4141 gtaattttct tgcctcttcc tcacgacctt ttgcgctgtc agcccccatt accactgcaa
     4201 tgagacgacg ctgcccatct acagccgaag caatgagatt aaacccggca ccagaagtat
     4261 gacccgtttt caggccgtca acattcatgg ttttatccca caataacccg ttacggtttt
     4321 gctgggtgat accgttccag gtgaggcttt tctcactgta catatgataa aactcgggct
     4381 cgccgtggat gatagcgcga gaaagcacag ctaaatcata agccgagcta tgctggccag
     4441 gtgcatccag accatgcact gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat
     4501 agttgttcat catttcaaca aactgccgtt gcccaccggc aatatagtca gccagtgcaa
     4561 cacaagcgtc atttccggaa tccacaatta aaccacggct taaatcacgt accgatacgc
     4621 gatcgccctc tttcaaaaac atcagtgaag aaccgacaaa caccggatta tctttcgccc
     4681 acgcatcacg ccccacggtg acaatatcgt ctggcgtaat gcgatgacta tcgatagcac
     4741 gatccacgac atagcccgtc atcagctttg tcaggctggc gggattgcgc tgttgatgct
     4801 cattacccgc ggtgagaatc tgaccggtgg tgtaatccat caatacccag gacccggcat
     4861 gaatctctgg aggctgaggt gaaaaaggaa tgttttccgc cgcaaaacca gacgataaat
     4921 taaaaacgaa caaagaagca gcaataataa gacggcgttt caacagcaaa ccctcaggtg
     4981 tttcaaatag ctgttctttt tacggaaata cttatgaact ggctggaata aagtgcaaga
     5041 aaatgtgact actatctcat ttttatctga catgatctgt tgccactcgc tgccaaattg
     5101 tcgcgctaaa gctgattagc acggtgatat ttgatactct ggcagacagc agaaataacg
     5161 gatttaacct aatgatgaat gacggtaagc aacaatctac ctttttgttt cacgattacg
     5221 aaacctttgg aacgcacccc gcgttagatc gccctgcaca gttcgcagcc attcgcactg
     5281 ataacgaatt caatgtcatc ggcgaacccg aagtctttta ctgcaagccc gcggatgact
     5341 atttacccca gcctggagca gtattaatta ccggtattac cccgcaggaa gcacgggcga
     5401 aaggagaaaa cgaagccgcg tttgccgccc gtattcactc gctttttacc gtaccgaaga
     5461 cctgtattct gggctacaac aatgtgcgtt tcgacgatga agtcacacgc aacgtttttt
     5521 atcgtaattt ctacgatcct tacgcctgga gctggcagca tgataactcg cgctgggatt
     5581 tactggatgt tatgcgtgcc tgttatgccc tgcgcccgga aggaataaac tggcctgaaa
     5641 atgatgacgg tctaccgagc tttcgccttg agcatttaac caaagcgaat ggtattgaac
     5701 atagcaacgc ccacgatgcg atggctgatg tgtacgccac tattgcgatg gcgaaactgg
     5761 taaaaacgcg tcagccacgc ctgtttgatt atctctttac ccatcgtaat aaacacaaac
     5821 tgatggcgtt gattgatgtt ccgcagatga aacccctggt tcacgtttcc ggaatgtttg
     5881 gggcatggcg tggcaatacc agctgggtgg caccgctggc gtggcatcct gaaaatcgca
     5941 atgccgtaat tatggtggat ttggcaggag acatttcacc attactggag ctggatagcg
     6001 acacattgcg cgagcgttta tataccgcaa aagccgatct tggcgataac gccgccgttc
     6061 cggttaagct ggtgcatatc aataaatgtc cggtactggc ccaggcgaat acgctacgcc
     6121 cggaagacgc tgagcgttta gggattgctc gtcaacactg cctggataac ctgaaagtgt
     6181 tgcgcgaaaa cccgcaagta cgcgacaaag ttgtggcaat ttttgcagaa gctgaaccgt
     6241 ttacaccatc agataatgta gatgcacagc tttataatgg cttttttagc gacgccgatc
     6301 gtgcagcaat gaaaatcgtg ctggagactg aaccgcgtaa tttaccggct ctggatatca
     6361 cctttgttga taagcgtatc gagaaactcc tgttcaacta ccgcgcccgc aacttccccg
     6421 gcacgttaga tgacaacgag cagcaacgct ggcttgagca ccgccgccag gtcttcacac
     6481 ccgagttttt acaaggctat gcagaagaga ttcagatgct ggcgcagctg tatgccgacg
     6541 ataaagaaaa agtggcgctg ttaaaagcgc tttggcagta cgcggaagag attgtctaat
     6601 gtgaagcctc cgccgctggt acggcggagg acaattttga gtcaggcttt ttgaacggtg
     6661 atgctccacg ctgcatcgcc aatctgctga taatcggtga tggcatgtcc ttcttctgcc
     6721 gcccactgtg ggattgcttc agttgcctgg gtgcaatcaa actcaattac caactcatcg
     6781 ccgctggcca tttctgccag tgctgctttt gcttctatga gtggaaacgg acagacctgc
     6841 gtcaccacat ccagcttttt aatcgccatc tttacacctt acttaatttg ccgcagcagt
     6901 tgccagtcgc gccttacgct gcggtcgaac atacacaagc caggacgcag tccagactcc
     6961 gagaatcata aatacaaggc caatccagcc ctgccaggtc atcattgcag tcatgaccag
     7021 tccattaccg atagagcaac cacctgcaat gctggcaccg aagcccatca gtacaccacc
     7081 taacccgcta cgtaatgttg tttgtgcatc agcagcgcga acgcggaact cacggctcgc
     7141 tttggcggca ataaatgacc ccacgaagct ccctaacacc aggaaaacgc cccagttaat
     7201 gtatttcacg tcacccgcga ccagaaattg cagaatattg gccgttgggg aagtgatgcc
     7261 taacccaaac atacgtccgg ttgcttcact caggggccag gctaaaagcg caatcaaacc
     7321 gatgagtaca gctgtgacaa agggatgcca gcgtttttca aacagaatat gagcgatccc
     7381 ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc ttcttcaact ctttcatcac
     7441 cacccagagc gttatcacca gcaaaacggc aaccaacggc cacacagaca aattaaaagt
     7501 atcagcaata gagttatgtt cagtagtgta gtgctgcaag gtttgattta aaccactggc
     7561 gtgtggagaa cgcatcaccg cactcatcac catataagtg aaaagcgcga tccaactgcc
     7621 gatcaatccc tcacccgcgc gataccaggt cccggtggca catccacccg ccagaacaat
     7681 tcccagcccg aaaagatacc cacctataac agtaccaagc cacgggaacg ccccggcttc
     7741 gtaagtcagt aggcccgcct gaattaacgc aaaaaccccc acgctttgta ccgaaattgc
     7801 aatcagcaag gcgtaaaaca tgcgattatt tttcgcgaca tacatatcgc gaaaaccacc
     7861 tgtcaggcaa aaacgcccac gctgcatgac aaatcccagc agagcaccac aaattagccc
     7921 gcttaatatc attgaaaaca taaattaatt aaccagatga atgttaataa ggaaattatt
     7981 catgactggt ggaatgcaga ccaataacca aattctctaa attagaacaa atggttatta
     8041 atgaggattt cgataaaaaa gaataaaaaa accggagcca aatattcatt cgactccggt
     8101 tgattattag aaaagatgat tatgctacgt cttcgtactg cggaaccggg ttacggaagc
     8161 tcttggttac gcatgccagg taaatcagac cgatagcagc ccagatcaga cccagaacca
     8221 ttgagctttc ttccaggtta acccacagcg caccaacggt cagcgcacca cacatcggca
     8281 ggaacagata ctggaagtga tctttcagcg tcttgttacg cttctcacgg atccagaact
     8341 gcgagatgac cgacaggtta acgaaggtga acgccaccaa cgcaccaaag ttaatcagcg
     8401 ccgttgccat taccaggtca aagttgattg ccagcagggc aatcgcgcca accaggatga
     8461 tattcatcgc cggagtacgc catttcgggt gtacataacc gaagaagctt ttcgggaaca
     8521 cgccgtcacg acccattacg tacatcagac gcgcaacgcc tgcgtgcgcc gccataccgg
     8581 acgccagtac ggtaatggtg gagaagatca gcgcgccaac ctggaacgct ttacccgcaa
     8641 cgtacagcat gatttcaggc tgtgacgcat ccggatcttt aaagcgagag atatccggga
     8701 agtacagctg caggaagtaa gttgcaaaga tgaagatcat gccgccaatc agcgcggtca
     8761 ggaaaatcgc acgcgggatc acgcgctctg catctttggt ttcttccgac aggttgctga
     8821 tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc cccggtaatc atcgggataa
     8881 catgcgcatc gccagaccag aacggacgag tgctcgccag cgtacccgct ccttcacctt
     8941 caaatacgcc ataaacaacc atgcccagga tcaccgcgat cagcactacc tgcaacacga
     9001 cgattacagt gttgaagttc gctacggatt tcagactacg caggttaaag gcggtcataa
     9061 aggccaccag cgccaccaca aacatccacg atggaatgga aggcaccaga gcttcaaaat
     9121 agattttcgc cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc agagaagacc
     9181 aacccaccat aaagccgaca gtcgggctaa tggatttctg ggcgtaagtg tatgcagagc
     9241 cagcagaagg atagcggcgg accagcttcc cgtagctcag agccgtaaac aggatcgcaa
     9301 tcaatgcgaa cgcataggct gtcggcacat gaccatccgt gaggcctgaa acgataccaa
     9361 aagtatcaaa cagcgtcatc ggctgcatat aggcaagacc catcattaca accggaacta
     9421 acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg
     9481 tcattctcct ttacggtgat aaccgtcgcg taagcaaaaa attgccccat ttttttggat
     9541 tcctcagcga caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct
     9601 cttggtatga atgattggtt tgaagcaaaa aataaccgac gctgatgaaa cgtcggtttt
     9661 tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga
     9721 gaggcttgtc ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata
     9781 aacaccggcg atcgcactat ttgctaaaat ttcatcccgc caccagccgc tggcaagccc
     9841 tgcggtctgt tcattccagc caatccagac gggttcaaag agagtttggg cgaccacttt
     9901 cttctcgatt aacgcgccac tatcgagaaa atgttgcgcc agataacggg gtaaatagcc
     9961 gcagccgagg ccgctaattt gcagctccag cttggtttta aaatcgaaaa cggtaatggc
    10021 ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag tgtcccccac
    10081 cacaatcgcc cgatatcgct taatgatacg acggtttaac ggctcttctt cctgggctaa
    10141 cggatgatga ggcgcgaccg caaagacttg ttccagatcg cccaaccgcg agaaaccaaa
    10201 ttcactggaa gatggtggct catgcatcgc gccaacaata atatccgctc gcccctgagt
    10261 cagggcatcc caggaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg
    10321 atgttgataa aaagcttcaa tcagtggcgc aagcagagaa aaaggaaacg tgtcgtccac
    10381 gccaatcacc agctcgtttt cccagccttc atgcagtttg atcgcctgct tttccagttc
    10441 ccgcacggta tgcagaactt cccgcccttt ctctaataac atttttccgg tgcgggtgaa
    10501 tttagcgcgg tgaccgctac gatcaagcaa ttgaatatta aggtcgcttt ccagcttgtg
    10561 aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg gccgacgccg ccgcaaaact
    10621 gccttctttt tctaaggcat cgaggatcat caacacatcc agcagtggtt tcatacacgc
    10681 ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt tactccagcg gcattaccag
    10741 cggatcggga tactggtatt caaaccccag ttcattacaa atccgactgc catcaataat
    10801 cttacctttg ccgctgtcca gactatttct gaactgcgga ggttccagcc ccagtaaacg
    10861 ggccatctgc ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat
    10921 gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg ccaataacat cttctaaatg
    10981 gactaaatta acaccatgtt caccatcagg cgcggttttt ccggcaaaga agcgtccggg
    11041 atgacgtccc ggtccgacaa ggcccgcaag acgcagaata tcgaccgaag taccgggtaa
    11101 attgtgcagc cagtcttcga gttcttctaa tactcttcca ctgttggtta ccggattacg
    11161 cggggtagtt tctttcaccg tgccttgcgc gtcgccatag acagacgttg agctggtgaa
    11221 aataatgcgg ggaatacgat gagccagcgc gctatccact aactcttgta ccgcttgtaa
    11281 atagaactca tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcatcggc
    11341 atccatcagg gcatccagat cgtcagaatc gcaaactaac tcaggctcca tgcgaagcag
    11401 atagctatca atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc
    11461 ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc ggcatgccta accaccctaa
    11521 cccgacaatt gcgacctttt tcatccgtta tctcctgata cacctaagat ctataaggct
    11581 acgctagcgt atcaaaactg acaattcatt ctatgaatga aatcgaaacg gttagcaaat
    11641 ataccattgc ctgatgcgct ccgcttatca gtcctacaaa aacatcaata tattgaattt
    11701 acgcaacatt tgtagaccag ataaggcatt cacgccgcat ccggcaggaa caaagcccac
    11761 tttagttaca gaacacaatg ttcttgaggg ttttaaagac atattttcac gatttctttt
    11821 ctaacgtagt cactccaggc cccctccttt ccccgctcat tcattaaaca aatccattgc
    11881 cataaaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca
    11941 tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa acaccaccat
    12001 catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat tcagatcttc
    12061 cagtg
//
LOCUS       DAOTRK010000138        12026 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.139, whole genome shotgun sequence.
ACCESSION   DAOTRK010000138 DAOTRK010000000
VERSION     DAOTRK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.139"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..133
                     /locus_tag="QIE41_003747"
     CDS             <1..133
                     /locus_tag="QIE41_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDS3532583.1"
                     /translation="NLLIIESFGEMAYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            123..296
                     /locus_tag="QIE41_003748"
     CDS             123..296
                     /locus_tag="QIE41_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDS3532584.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(345..1418)
                     /locus_tag="QIE41_003749"
     CDS             complement(345..1418)
                     /locus_tag="QIE41_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDS3532585.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWYDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLICVIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1490..4234)
                     /gene="torS"
                     /locus_tag="QIE41_003750"
     CDS             complement(1490..4234)
                     /gene="torS"
                     /locus_tag="QIE41_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDS3532586.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYGDLLALY
                     QQDNEISHRLQTLTQNNIAQFAQFSSEVSQLVDSIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGELWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMSGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVHLDGL
                     RLLLIEDNPLTQRITVEMLNNSGAQVVAVGNAAHAIETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDDIGIARSSQNSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            4317..5345
                     /gene="torT"
                     /locus_tag="QIE41_003751"
     CDS             4317..5345
                     /gene="torT"
                     /locus_tag="QIE41_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDS3532587.1"
                     /translation="MRVLLLLLLSLFMLPGFSADNLLRWHDAQHFSVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WSHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5318..6010)
                     /gene="torR"
                     /locus_tag="QIE41_003752"
     CDS             complement(5318..6010)
                     /gene="torR"
                     /locus_tag="QIE41_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDS3532588.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLTRQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6140..7312
                     /gene="torC"
                     /locus_tag="QIE41_003753"
     CDS             6140..7312
                     /gene="torC"
                     /locus_tag="QIE41_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDS3532589.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAGK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7312..9858
                     /gene="torA"
                     /locus_tag="QIE41_003754"
     CDS             7312..9858
                     /gene="torA"
                     /locus_tag="QIE41_003754"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDS3532590.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRASAAQAAT
                     EAVTSKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYSSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPSVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            9855..10454
                     /gene="torD"
                     /locus_tag="QIE41_003755"
     CDS             9855..10454
                     /gene="torD"
                     /locus_tag="QIE41_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDS3532591.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(10606..10911)
                     /gene="cbpM"
                     /locus_tag="QIE41_003756"
     CDS             complement(10606..10911)
                     /gene="cbpM"
                     /locus_tag="QIE41_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDS3532592.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10911..11831)
                     /gene="cbpA"
                     /locus_tag="QIE41_003757"
     CDS             complement(10911..11831)
                     /gene="cbpA"
                     /locus_tag="QIE41_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDS3532593.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2863 a         3111 c         3212 g         2840 t
ORIGIN      
        1 taacctgttg attattgaat ctttcgggga gatggcttat aacatttctt acctgaccag
       61 ggtaccggga accaacacct tactggcgtg ttgctgtctt ttaagaccag aagaggttaa
      121 cagtgaatat tgaagagtta aaaaaacaag ccgaaacgga aatcgctgac tttatcgcgc
      181 aaaaaatcgc cgagctgaac aagaatacag ggaaagaagt ctctgaaatt cgcttcactg
      241 cacgagaaaa aatgaccggg cttgaaagtt atgatgtcaa aatcaaaata atgtgatttt
      301 gtgaacatca ccccgtgcga ggtgatgttc cgcttgttgc taatttagtg cccaatcatt
      361 ggcgcttgcg gaattaagcg tcggtacaat tcttccggca ccgggctttg ccatacaccc
      421 gcatacattg cgtaaccaat caccgcaaac ataatcccca gaaccagtag cgtcattaac
      481 cagccagaca acgcaaaggc ttttttattt gccgcaggtt tttgcagtga aaaggtcaat
      541 gttgaggcta ccggacatga ctctacgcaa gtcatacaac cggtacattc cacggttcgt
      601 acctgaatta atttatcgac cgggatccgc gatgggcaat tttttgcgca tttgccacaa
      661 tcgatacaac tttcggcatt gcgacgaatc ttaaacggtg ataatagcga aaccacgccc
      721 atcagcgcgc catatgggca aagataacga caccaggcat ggcgaataaa caggctggca
      781 atcagcaaaa cgatcacgca gattaatgtc gctgtcccca tatggcgaaa gaaatcgagc
      841 attttaacgt ccatcaccac gctgtagggc gacaacataa aatagtgaat cgcctgagcg
      901 ggcatcaata acgcaatata gagaaaaaaa ctcaacagca aatacttcac gccgcgcaga
      961 ggaatatcca gccagcgggg aaggacacat tgccgaccaa acagtttgtt accgagatcg
     1021 ccgattaatt cagaaagcgt accaaccggg cataaccatg aacaaaaggc ctttttgagt
     1081 aatagactga tgacgataaa agcgaccaat aacagcatcg cggcggcgtg gacggacggt
     1141 aactgacctg ttgcaaggct atatttcaga ttcatcagac cggcaatcgg tagccagcct
     1201 tcgatgcctc ctggcctggc gacaaatgtc gtactacctg ccgtttcgta atagcgcacc
     1261 caataccaga acgtgatggc aatataaata ttcattgcca acagtaataa ttgcgtcgct
     1321 ttacgccagg tcgtggcatt gcgccagtca taccacggta atttgccgcc cgtcgtgcct
     1381 ggccgccgct gccagcgggt tcttcttttc tctgtcatga ttttgccagt ccgttaagtt
     1441 gcataccaaa tgccactatt ctagttgttc ttaactggct gatattgatt caaatcgcgt
     1501 tcaggtcttt cttattcaac catgcttcca gagcggcaac actgcgtgta atttcttcgt
     1561 gtggaagggg ggcagataat ggctgctgct ccagttgtgc gcatagctgg ctggcgctac
     1621 gcattcccag actcgagcaa ctgcttttta gctgatgtgc ggcacgcttt attttttcgc
     1681 tgttctggct ggagcgggcg atgccgatgt catcgagaag cggcagggca tgttgtttaa
     1741 ataatgccag ccattcgtgg atcttctccg tccccatcaa ctgagcatct tcattgagtt
     1801 gcgatacatc cagcggttga tcgttattga cttgcagttg gagatagtgc gccagtaact
     1861 gaccgagcac ttcacgcggc accggtttag ggataatccc gcgaaataat gaactggtac
     1921 gctggcgcaa cgtttcgtcg atgacatgag cgctaaagcc aatcaaaacc agcgacggat
     1981 attgctgtgc cagttgccgg gcaagcgtaa tgccgtcgat atcgggcaga tcaaaatcca
     2041 ccagtgcggc agcaaacggt tcgctatttt gcaacgtctc tattgcgtgc gcggcatttc
     2101 ctaccgcaac gacctgcgcc ccactgttgt tcagcatctc aacggtaatt cgctgggtta
     2161 gcgggttatc ttcaattaac agcaaacgta aaccgtccag atgcaccgcc tgattgactg
     2221 tttttggcac gggtgcagtg gcaacacgta acggcaagcg taaacaaaaa cagcttccaa
     2281 cctccggcgt gctggtggcg ctcagttcgc cgctcatcgc ctgggccaga cggctactga
     2341 tagtcagccc cagcccggtg ccgccgcgtt tgccgctaac ctgaacaaat ggctggaaga
     2401 tttcagccag tttcgcggga tcaataccgc agccgctgtc ttccacttcg accagccaca
     2461 gctcgccatc agtgcgacta cgcaggataa tgtgcccttc gtcggtgaaa cgcagggcgt
     2521 tgctcaacag gttggttatg acctgacgaa tacgccgcgg atcgcccatt aacgcagtcg
     2581 gtacatcgtc ggcaatttct gttgccaggc gaatcgggcg acctttcacc cttccgctca
     2641 ttaattgcag ggtactttcc agcagcgggc gcggttcaaa gggttcatcg ctgaccgaaa
     2701 cattcttgcc gcctgcttcg atagccgaat aatcgagaat atcgttgagg atggtcagca
     2761 acgattcacc agagtcggta attgcccgca aatcatcacg ctgggcatta agtgcggggt
     2821 tatctgccag cagttgcgcg gtgccaagaa taccgtacag cggtgtgcgg atctcatggc
     2881 tcatcgccgc cagaaacgcc gattttgcct ggctggcttt ttctgcttcc gctcgtgcct
     2941 gtcggtgttc tatcaccagt tcctgcaatt cagctgtacg cgctttgacc tgcgccgcca
     3001 gctgttcgcg gtggcgattc agtgcatgaa cactgctgcg aaacgcatcc atcagccgcc
     3061 cgatggtgtc cagctcccgc acgccagcgg tttccggaaa aggggagtcg atatcaccgt
     3121 ccagtaagcg ttgcagcgcc tgcgtttgtt cggcaagtgg acgcgtgact gagcgataaa
     3181 ccacgcgcca gaggatcaga atcaatgcac aaagtgaaac tatccccagt aataacaggc
     3241 tgtattgccc gcgtgcactg gctttttcca gatgtgccag cccgtgctga ttgcgcagct
     3301 caatactgtc gaccagctga ctgacttcgc tactaaactg cgcgaactgg gcgatgttat
     3361 tttgcgtgag agtttgcagg cgatgactga tttcattgtc ctgttgatac agcgccagca
     3421 aatcgccata ttggcttacg gtagttaacg ttgttgcgac ctgagcacga actcccggat
     3481 cttcaatgcg tatttgccga cgttgcagaa ttttcaccgc attattgagc tgcttttcca
     3541 gcgttggcgc atttttctgg atctgttcca accccagatt catcaccatt tgctgcaccc
     3601 gcagggcgct aaggcgcagt tcattcatct ggttaacata ctcaagatcg atatcaatca
     3661 gccgatcgag tgcactttct gcggcctgac gctgatgttg ttcaatcaaa tcgtaaatcc
     3721 cggcctgggt cgctccggcg gaggttgccg cattattcgc ctgaccttgc gccaggcgtg
     3781 cgatttcatc ggcggcggcg actatctgct gactgagttg ctgttgttgc tggcgcagtt
     3841 gcaaacgctg ccccaccagc tccccttgtt gacgtaacga acgggaaatc tcctgctcct
     3901 gttgttcaat agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa
     3961 tcttcaggct ttgtgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg
     4021 cactggtcag gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg
     4081 ccatattcat ggtaggaatc aatgcctgag tgttgtcttt ttccacctgg ctgataaagc
     4141 gcaggttgta ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc
     4201 ccatccagag tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg
     4261 ttgagtcgca aagcggaatg catctagcat aaagccttat tattgatgag gctatcatgc
     4321 gcgtactgct attgttactt ctttcccttt tcatgttgcc tggtttttcg gctgataacc
     4381 tgttgcgctg gcatgatgcg cagcatttct ctgtgcaagc ctctacgccg cttaaagcca
     4441 aacgcgcatg gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt
     4501 tgaactatgg tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg
     4561 caggcggcta cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg
     4621 gcgcagaggc cattttgctc ggtagtagca cgacgtcatt tcccgatctg caaaagcagg
     4681 tagcaagtct gccggtgatc gaactggtaa atgctattga tgctccccac gtgaaaagcc
     4741 gcgttggtgt gccctggttt cagatgggct atcaaccagg acgatatctg gtgcaatgga
     4801 gccacggtaa accactgaac gtgctgttga tgcccggacc cgataacgcc gggggcagta
     4861 aggagatggt cgagggtttt cgcgcagcca ttgccggaag cccggtacgt attgttgata
     4921 ttgcgcttgg cgataacgat attgaaatcc agcgtaacct gttgcaggag atgctggagc
     4981 gccatccaga aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatggggg
     5041 aagggcgtaa tctgaaaaca ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt
     5101 atcgcgggct gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg
     5161 gggaactggc ggttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg
     5221 tcagcccacc gattttagtt ctgacgccga aaaatgccga ccgtgaacat atccgccgct
     5281 cgctgtcacc agggggattt cgtccggtct atttttatca gcacacatca gcggctaaga
     5341 aataaccttc accatgttgc gtcaccagta aatccgcgct gagtttatgg cgtaaacgac
     5401 gaattaacac atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gagagcatac
     5461 gcagcagacg ttcacggcta agaatttcgc ccggattcgt cacaaatgcc accaacattt
     5521 catactctgc gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca
     5581 cattcaggca ataaccggca aagcgatagc agttgtcctg tgtgtgcggt tgagcttgtc
     5641 gcgtgaggtc gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca
     5701 gcggtttggt gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat
     5761 cgctgcgtcc cgtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtca
     5821 acatcaggcc attttcatcg ggcaaattga tatccagcag aattaaatcc accggctgat
     5881 tctgcataat ttcccgcagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct
     5941 gagtgaagta ggattgtaat cgcgcctggg taaccggctc atcttcaaca ataacaatgt
     6001 gatgtggcat cagagggttt tactcattct gttcatatct gttcatattc tgccgtaagc
     6061 cgttcatcct gaccagtgcc gctgttcata tttactcatt aagatcgctt cactaaacca
     6121 taattttaca ggggctatta tgcggaaact ctggaacgcg ctacgccgac ccagtgctcg
     6181 ttggtcggta ctggcgctgg tcgctattgg gattgtgatt ggcattgcgc tgattgtatt
     6241 gccacacgtt gggatcaaag tcaccagcac aaccgaattt tgtgtcagtt gccacagtat
     6301 gcaaccggtg tatgaagaat ataaacagtc ggtgcatttc cagaatgcct ccggcgtgcg
     6361 agctgaatgc catgactgtc atatcccgcc ggatattcca ggcatggtga agcgcaaact
     6421 ggaagcgagc aacgatatct accagacctt tattgcccac tccattgata cacctgaaaa
     6481 attcgaagcc aaacgcgcgg aacttgccga gcgtgaatgg gcgcgaatga aagaaaacaa
     6541 ctcggcaacc tgccgctcct gccataacta cgacgcgatg gatcatgcca agcagcatcc
     6601 tgaagcggca cgtcagatga aggtggcagc gaaagataat caatcctgca tcgactgtca
     6661 taaaggtatt gcccaccagt tgccggatat gagtagtggc ttccgtaagc agttcgatga
     6721 gctgcgcgcc agtgctaatg acagtggtga cacgctgtac tccattgata ttaagccgat
     6781 ttatgcgggg aaaggcgata aagaagcctc tggttctctg ctgcctgctt cggaagtgaa
     6841 agtccttaaa cgtgacggcg actggctgca aattgaaatt accggctgga cggaaagcgc
     6901 cggacgtcag cgtgttctca cccagttccc aggtaaacgc atctttgttg cctcgattcg
     6961 tggtgatgtg cagcagcagg tgaaaacgct ggagaaaact accgttgccg acaccaatac
     7021 tgagtggagc aaattgcagg ccactgcgtg gatgaagaaa ggcgacatgg taaacgatat
     7081 caaaccgatc tgggcttatg cggattcgtt gtacaacggc acctgtaacc agtgccacgg
     7141 cgcaccggaa atctctcact ttgacgctaa cggttggatc ggcacgctca acggcatgat
     7201 tggctttacc agcctcgata aacgtgaaga acgcaccttg ttgaaatatc tgcaaatgaa
     7261 tgcgtctgac accgcaggta aggctcacgg cgataagaag gaagaaaaat aatgaacaat
     7321 aacgatctct ttcaggcatc acgtcggcgt tttctggcac aactcggcgg cttaaccgtc
     7381 gccgggatgc tggggccgtc attgttaacg ccgcgccgtg cgtctgcggc gcaagcggcg
     7441 actgaggctg tcacctcgaa agagggcatt cttaccgggt cgcactgggg ggctatccgc
     7501 gcgacggtga aggagggtcg ctttgtggcg gcaaaaccgt tcgaactgga taaatattcg
     7561 tcgaaaatga tcgccggatt gccggatcac gtacacaacg cggcgcgtat tcgttatccg
     7621 atggtacgcg tggactggct acgtaagcgc catctgagcg acacctccca gcgcggtgat
     7681 aaccgttttg tgcgcgtgag ctgggatgaa gccctcgaca tgttctatga agagctggaa
     7741 cgtgtgcaga aaacgcacgg gccgagtgcc ttgctgaccg ccagtggttg gcagtcgacg
     7801 gggatgttcc ataacgcttc ggggatgctg gcgaaagcga ttgccttgca tggtaatagc
     7861 gttggtacgg gcggagatta ctctaccggt gctgcgcagg tgatcctgcc gcgcgtagtc
     7921 ggttcgatgg aagtgtatga acagcaaacc tcctggccgc tggtattgca gaacagcaaa
     7981 accattgtgc tgtggggctc cgatttgctg aaaaaccagc aagcgaactg gtggtgcccg
     8041 gatcacgatg tttatgaata ttacgagcag ctaaaagcga aagtcgccgc cggtgaaatt
     8101 gaggtcatca gcatcgatcc ggttgtcaca tccacccatg agtatctggg gcgcgagcat
     8161 gtgaagcaca ttgcggttaa cccgcaaact gacgtgccgc tgcaactggc gctggcgcat
     8221 acgctgtaca gtgaaaacct gtacgacaaa aacttcctcg ctaactactg tgtaggtttt
     8281 gagcagttcc tgccgtatct gctgggtgag aaagacggtc agccgaaaga tgccgcatgg
     8341 gctgaaaaac tgaccggcat tgatgccgaa accattcgtg ggctggcgcg gcagatggcg
     8401 gcgaacagaa cgcaaattat tgctggctgg tgcgtgcagc gtatgcagca cggtgaacag
     8461 tgggcgtgga tgattgtcgt tctggcggcg atgctggggc aaattggcct gccaggtggt
     8521 ggctttggtt ttggctggca ctataacggc gcaggcacgc cggggcgtaa aggcgttatt
     8581 ctgagtggtt tctccggctc tacgtcgatt ccgtctgttc acgacaacag tgactacaaa
     8641 ggttacagca gcaccattcc gattgcccgt tttatcgatg cgatcctcga accggggaaa
     8701 gtgatcaact ggaacggtaa atcggtaaaa ctgccgccgc tgaaaatgtg tatttttgcc
     8761 ggaactaacc cgttccatcg ccatcagcag atcaaccgca ttattgaagg ctggcgtaag
     8821 ctggaaacgg ttatcgccat agataaccag tggacctcaa cctgccgctt tgccgatatc
     8881 gtactgcctg cgaccacgca gtttgagcgt aacgatctcg accagtacgg caaccactcc
     8941 aaccgtggca ttatcgccat gaaacaggtc gtgccgccgc agttcgaggc gcgcaacgac
     9001 ttcgatattt tccgcgagct gtgccgtcgc tttaatcgcg aagaagcctt taccgaaggg
     9061 ctggacgaaa tgggctggct gaaacgcatc tggcaggaag gtgtacaaca gggcaaagga
     9121 cgcggcgttc atctgccagc gtttgatgac ttctggaata acaaagagta tgtcgagttt
     9181 gaccatccgc agatgtttgt tcgtcaccag gcattccgcg aagatccgga tctcgaaccg
     9241 ctgggtacgc cgagtggcct gattgagatc tactcgaaaa ccatcgccga tatgaactac
     9301 gacgattgtc aggggcatcc gatgtggttt gagaaaatcg aacgctccca cggcgggccc
     9361 ggctcgcaga agtatccgtt gcatctgcaa tctgtgcatc cggatttccg acttcactcg
     9421 cagttatgtg agtcggaaac gctgcgtcag caatatacgg tagcgggcaa agagccagtg
     9481 ttcattaacc cgcaggatgc cagcgcgcgt ggtattcgta acggtgatgt ggtacgcgtc
     9541 tttaacgctc gtggccaggt gttggctgga gcagtggttt ctgaccgcta tgcacccggc
     9601 gtggcgcgaa ttcatgaagg ggcatggtac gatccagata aaggcggcga gcctggtgcg
     9661 ctgtgcaaat acggtaaccc caacgtgctg accatcgaca tcggtacatc gcagctggcg
     9721 caggcgacca gtgcgcacac tacgctggtg gaaattgaga agtacaacgg aacagtggag
     9781 caagtgacgg cgtttaacgg ccccgtggag atggtggcgc agtgcgaata tgttcccgcg
     9841 tcgcaggtga aatcatgacc acgctgacag cacaacagat tgcctgtgtt tacgcctggc
     9901 tggcgcagtt gttctcccgt gaactggacg atgaacaact gacgcaaatc gccagtgcgc
     9961 agatggctga atggttttcg ttgctgaaaa gtgaaccgcc gctcactgcg gcggtgaatg
    10021 agctggaaaa ccgtattgcc acgctgacag tacgtgacga tgcccgtctg gaactggccg
    10081 ctgacttttg cggtctgttt ctgatgaccg acaaacaagc ggcgctgcct tatgcatcgg
    10141 cctacaaaca ggacgagcaa gagattaaac gcttgttagt tgaggcaggg atggaaacca
    10201 gcggcaattt caacgaaccg gcagatcatc tggcgatcta tctcgagttg ttgagtcatc
    10261 tgcatttttc gctgggagag gggaccgttc ctgcgcgaag aatcgacaat ttgcggcaaa
    10321 aaacactgac ggcgctgcga caatggttac cagagtttgc tgcgcgttgc catcagtacg
    10381 acagtttcgg tttttacgcg gcactaagcc agttattgct ggtgttagtg gaatgcgacc
    10441 accaaaacag ataacatcgt ttgttggcct gataagacgc gtttagcgtc gcatcaggca
    10501 ttatggcgca gttgccggat gcggcatgaa cgtcttatcc ggcccacagg aactgtaatc
    10561 gttgtagacc ggctaagatg cgtcatcgca tccggcaaac acacatcacg gatgagctac
    10621 aaaccgggaa agccgctggc gcagcaggcg gttttcctgc ttcaggtgcg caatatcatc
    10681 cattaacgtc agcgccaccg cgatccccgg ccagtccaga gcgagttcat gacgcaggcg
    10741 taccgcgcgt tgcaccacaa tggcggcatg gtcgtcaaat acccaggttg tttcctggat
    10801 ctcacgcggt tcaaccaccc ccaaaccgac aatttcattt agctcctctt cagagatgcc
    10861 ggtatgcagg caaaattcgg taatagtaaa agtcaccgta acattagcca ttatgctttc
    10921 ccccaatctt tacgtggatc aaaagacgac tgggcgtctg ccagttgctg ccatagcgcg
    10981 gcagtgtttt catccggttt cggcggcatc acgattttca gtaccgcata cagatcgccg
    11041 gtctgttttt tgctcaccag acctttgcct ttaacgcgca atcgttgccc tgcctggctg
    11101 ccaggcggga tagtcagcaa aatgctttct ttcagtgttg gaacggtgac tttagcaccc
    11161 agcgccgctt cccacgggct aaccggcacc acaatttcca gatcctggcc gacaatatca
    11221 aacagcggat gtggcgcaat atgaatcacc agccacaaat cgccattcgg gccgccattt
    11281 tcgccaggcg tcccctggcc tttcaggcgg atgcgttgac cattgccgac gcccgccggg
    11341 atcttcacat tcagcgtttt cgggatttcc tgttcgatca tgccaaaggc gttataaacc
    11401 ggcaggttat agctgatggt acgcttatgc tcagtaagcg tttcttcgag gaataccgcc
    11461 acttcgattt caatatcgtg gccgcgtgtg gcggggcgtt gacggctctg gcgggcatgc
    11521 tgaccgaaaa ttgacgagaa gatatcgtca aaatcttcgg cgttaaaact ctgaccgtcg
    11581 ccatgctgga actgacggct aaattgcgga tcgttgcgat gttgccacat ctgatcatac
    11641 tcagcgcgac gttgttcatc acttaacact tcccaggctt cagcgacctc tttgaagcgg
    11701 gcttcggcat ccggttcttt gctgacatca ggatggtatt tgcgggccag tcgacgatag
    11761 gcggtcttga ttgtcttgag atcgtccgtc ggtttcacgc ccatgatggc gtaataatcc
    11821 tttaattcca tagcgttatc tcgcgtaaat caactcaaat tgaaggaacc cctgtaaggt
    11881 aactcctata agtgtagggt aatcctcaaa atttcatatg ccaacacaga atatgttatt
    11941 gaaatcatcg cggagaggag gtcgcctcaa ggtggggtgt tgaacatatt ttaaacaggt
    12001 gaaaaagggt gagcgatttt tgatag
//
LOCUS       DAOTRK010000139        11952 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.140, whole genome shotgun sequence.
ACCESSION   DAOTRK010000139 DAOTRK010000000
VERSION     DAOTRK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.140"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..47)
                     /gene="yobI"
                     /locus_tag="QIE41_003758"
     CDS             complement(<1..47)
                     /gene="yobI"
                     /locus_tag="QIE41_003758"
                     /inference="COORDINATES: protein motif:HMM:NF038179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HDS3532594.1"
                     /translation="MYIFITHFFTEYVIL"
     gene            118..720
                     /locus_tag="QIE41_003759"
     CDS             118..720
                     /locus_tag="QIE41_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDS3532595.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(722..1243)
                     /gene="ruvC"
                     /locus_tag="QIE41_003760"
     CDS             complement(722..1243)
                     /gene="ruvC"
                     /locus_tag="QIE41_003760"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDS3532596.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFEPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1278..2018)
                     /locus_tag="QIE41_003761"
     CDS             complement(1278..2018)
                     /locus_tag="QIE41_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDS3532597.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2047..2499)
                     /gene="nudB"
                     /locus_tag="QIE41_003762"
     CDS             complement(2047..2499)
                     /gene="nudB"
                     /locus_tag="QIE41_003762"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDS3532598.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2617..4389)
                     /gene="aspS"
                     /locus_tag="QIE41_003763"
     CDS             complement(2617..4389)
                     /gene="aspS"
                     /locus_tag="QIE41_003763"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDS3532599.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANNPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4699..5265
                     /locus_tag="QIE41_003764"
     CDS             4699..5265
                     /locus_tag="QIE41_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDS3532600.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAVKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5262..6080
                     /locus_tag="QIE41_003765"
     CDS             5262..6080
                     /locus_tag="QIE41_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDS3532601.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKS
                     EVVLRWREQTTDNFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRDLPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6133..6528
                     /locus_tag="QIE41_003766"
     CDS             6133..6528
                     /locus_tag="QIE41_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDS3532602.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6569..7312
                     /gene="cmoA"
                     /locus_tag="QIE41_003767"
     CDS             6569..7312
                     /gene="cmoA"
                     /locus_tag="QIE41_003767"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDS3532603.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HQAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7309..8280
                     /gene="cmoB"
                     /locus_tag="QIE41_003768"
     CDS             7309..8280
                     /gene="cmoB"
                     /locus_tag="QIE41_003768"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDS3532604.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8315..10744)
                     /gene="torZ"
                     /locus_tag="QIE41_003769"
     CDS             complement(8315..10744)
                     /gene="torZ"
                     /locus_tag="QIE41_003769"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDS3532605.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAVDQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLAGKSDNTPKSAAWAAEISGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGRDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNRRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10769..11869)
                     /locus_tag="QIE41_003770"
     CDS             complement(10769..11869)
                     /locus_tag="QIE41_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDS3532606.1"
                     /translation="MRGKKRIGLMFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGVHIYPFKTSRIGELATVNPGTD
                     LIVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2743 a         3166 c         2905 g         3138 t
ORIGIN      
        1 tttaatatta catattcagt gaagaaatgc gtaataaaaa tatacattgc gcctcctgaa
       61 aaaataaatt ttttatgcta ttacgtatat tcgtatctat ttcaatggaa tgacaacgtg
      121 aatattaatt accctgctga atatgaaatt ggagatatcg tctttacatg tataggtgct
      181 gccttatttg gtcaaatatc agctgcatca aattgctgga gtaatcacgt cgggatcatt
      241 atcggtcata acggtgaaga cttcctggtt gcagaaagcc gggttcccct ttcaaccatc
      301 accacgctat ctcgttttat taaacgttcg tctaatcaac gctatgctat aaagcgatta
      361 gacgctgggc tgacagaaca acaaaaacaa cgaattgttg aacaggttcc ctcccggcta
      421 cgcaaacttt accacaccgg ttttaaatac gaatcttcgc gccagttctg ttcaaaattt
      481 gtttttgata tttataaaga ggcgctatgt attccggtgg gtgaaataga gacgtttgga
      541 gaattgttaa atagcaatcc gaatgcaaaa ctcactttct ggaaattctg gttcctgggt
      601 tctattccgt gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca tccgggtttg
      661 gtgttgattc atgcggaggg agtggaaacg cctcagccgg aactgaccga ggcggtataa
      721 cttaacgcag tcgccctctc gccaggttca gccgcgattc gctcatctgc atcgcattct
      781 gactgacgtg acaatgggta atcgcgatag ctaatgcatc ggcagcatcc gcctgtggat
      841 tagcgggcag tttcagcaag gtgcggacca tatgctgcac ctggcttttt tcagcactac
      901 caatacctac caccgtttgc tttacctgac gtgccgcata ttcaaatacc ggcaattcct
      961 gattcaccgc cgccacaatc gccacgccgc gcgcctgacc cagtttcagg gctgagtcag
     1021 cgttcttcgc cataaagact tgttcaatgg cgaaataatc aggctcgaac tgggtgatga
     1081 tttccgtcac gcccgcatag atgagcttca gacgagacgg taaatcatcc actttggtgc
     1141 gtatgcatcc gctacccagg taggagagtt gcctgcctac ctggcggatg acgccatagc
     1201 cggtcacgcg cgaacccggg tcaatgccga gaataatagc catcacgcgt ctccgttttg
     1261 ctgtttagca ggccccatca gagagtcgct gcaacctcat cggagatttc accgttatgg
     1321 tagacttcct gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat cagtttcggt
     1381 gcggtttcag catccatatc agctttggtg gacgggatca tggaaacttc cgcgctgtct
     1441 gctttcagac ctgccgcttc cagagcgtcg cgtactttac ccatttcttc ccatgcagtg
     1501 tagacgtcaa tcgcgccgtc atcataggtc acaacgtctt cagcacccgc ttccagagct
     1561 gcttccatga tggtgtcttc atcgcctttc tcgaaggaga tcacgccttt tttgctgaac
     1621 agataggcta cggaaccatc agtaccgagg ttaccaccac atttgctgaa tgcatgacgc
     1681 acttcagcaa cggtacggtt gcggttgtca gacagacatt caatcatgat tgccgtgccg
     1741 ccaggaccgt aaccttcgta gatgatggtt tccatgtttg catcatcatc accgcccaca
     1801 ccacgtgcaa ttgcgcggtt cagagtgtca cgggtcatgt tgttagacaa tgctttatca
     1861 attgctgcac gcagacgcgg gttagcgtcc gggtcaccac cgcccagctt agccgcggtt
     1921 accagctcac gaatgatttt agtgaagatt ttaccgcgct tagcatcctg cgcagcttta
     1981 cgatgtctgg tgttggccca tttactatga cctgccataa aaatatctcc agatagccct
     2041 gcctgttcag gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc tccaggactt
     2101 agtgagcgcc gccgcagcag gcgcatcaag ccacttgtag gccagatgtt cagtgaagac
     2161 gacctggcgt tcgtgcggga gcgcaagaca gaaccatgat tccgtattac gcgtcacgcc
     2221 cggcgcatag cgatgacgta aatgtgaaaa aatttcaaat tctaccgtgc gctgacagtc
     2281 aattaaggtc agttgttcag cgacaacatc gatggtgacc tcttccttta cttcgcgcat
     2341 ggcagcttgc ggcgcggttt caccctcttc cacgctgccg gttaccgact gccagaaatc
     2401 gggatcgtca cgccgctgca acatcagcac ccgtttcgta tcttgcgcat agatgaccac
     2461 taagatcgaa acgggacgct tatacacttt atccttcacg ctgcctcttc gttgactgcc
     2521 ttcgctcatc ccattcacat agttatctat gctcatggga gttcactcag ttgccgcctc
     2581 gatgcaacgc gaatgatttc gtgtatttga gtcatatcag ttattctctg ccttcttcac
     2641 aacctgaatg ctcagctcag ccagtgcagt cgggttagca aagctcggtg cttcagtcat
     2701 cagacacgcc gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc
     2761 ggtcagcagc atcgtcagac ggtcaagacc gaatgccaga cctgcgtgcg gcggagtacc
     2821 gtatttcagg gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc
     2881 cagaatacca aacaccgtct gctgcatatc accattatgg atacgcacag aaccaccgcc
     2941 cacttcgtaa ccattgatga ccatatcgta agcgttcgcc accgcatttt ctggtgcagc
     3001 tttcagttct gcagcagtca tgtctttcgg tgaggtgaac ggatggtgca ttgccgtcag
     3061 gccgccttca ccgtcgtctt caaacatcgg gaaatcgata acccacagcg gtgcccattt
     3121 gctttcgtcg gtcagaccaa ggtctttacc cactttcagg cgcagtgcgc ccatcgcgtc
     3181 ggcaacaatt ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt gcgcggcagt
     3241 acgatccagg atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat
     3301 accttccaga cctttcgcgc gttcgttaac tttgatgtaa gccagacctt tcgcgccgta
     3361 gattttaacg aagttaccgt attcgtcgat ctgcttacgg gtcagcgatg cgccacccgg
     3421 aacacgcaga gcggcaacgc gccctttcgg attgttcgcc ggacctgcaa atactgcaaa
     3481 ttcaacagat ttcagcagat cggcaacgtc agtcagttcc atcgggttac gcagatccgg
     3541 tttatcagaa ccataacggc gttctgcttc cgcaaaggtc attaccggga aatcgcccag
     3601 atccacgccc ttcacttcca gccacagatg acgcaccagc gcttccatca cttcacgcac
     3661 ttgcggcgcg gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacgatc
     3721 agcgcgcagg tcttcgtcac ggaagcattt aacgatctga tagtaacggt caaagccgga
     3781 catcatcagc agctgtttga acaactgtgg ggattgcggc agtgcgtaga atttaccttt
     3841 gtgcacacga gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat
     3901 cggagtttcg atgtcgagga agccgtggtc atccataaaa cggcgcacca ggctggtgat
     3961 tttagcgcgg gttttcaggc gctgagccat ttccgggcga cgcaggtcga ggtagcggta
     4021 tttcagacgc gcttcttcgg tgttgacgtg gttagagtca agcggcagaa catctgcacg
     4081 gttgatgata gtcagcgagg acgccagtac ttcgatttcg ccagtcgcca tatcgcggtt
     4141 aatatttttt tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga actcattacg
     4201 cagttcagag gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat
     4261 accttcgcgg tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac
     4321 ccaaccacac agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc
     4381 tgtacgcatg agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga
     4441 ccaagtcgca gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg
     4501 aaattctgcc gcaccgtaag agtctggccc gcgctggaac gcctcgttac cactttatat
     4561 cgggcctgaa atcagactct acgccagttt gctataaagg tgttgcccga actcataaaa
     4621 attaacaaaa tttgtcgttc cgccatcggc taatcgcatt aaggtgagag gcacgatttt
     4681 gttttgtcag gagtcatcat gcttgaactt aatgctaaaa ccaccgcgct ggtggtgatt
     4741 gatttacaag aaggcatctt accttttgcc ggaggtccac atactgccga tgaggtggtt
     4801 aatcgcgccg ggaagctggc ggtgaaattt cgcgccagcg gtcagcccgt gtttctggtg
     4861 cgcgttggct ggtctgccga ttacgccgaa gcattaaaac agccggttga tgccccttcc
     4921 cccgctaaag tgttgcccga aaactggtgg caacatcctg ctgcattagg tgcaactgac
     4981 agtgatatcg aaatcatcaa acgtcagtgg ggggcgtttt acggtacaga tctggagttg
     5041 caattacgcc gccggggtat cgatacaata gtattatgtg ggatctcgac caatatcggt
     5101 gttgaatcca ccgcccgcaa tgcctgggaa ctcggtttta atctggtgat tgccgaagac
     5161 gcctgcagcg ccgccagcgc cgagcagcac aataacagca tcaaccatat ctacccacgc
     5221 atcgcccgtg tgcgcagtgt ggaagagatc ctcaacgcat tatgatttac atcggtctgc
     5281 cgcaatggtc gcatcctaaa tgggtgcggt tggggatcac cagccttgaa gagtatgccc
     5341 gccactttaa ctgtgtggag ggcaacacca cgctttacgc cctgccgaaa tccgaggttg
     5401 tcctgcgctg gcgtgagcag accacagata attttcgctt ctgttttaag tttccggcga
     5461 ccatttcgca tcaggcagca ttacggcatt gcgatgattt agtgactgaa tttttgaccc
     5521 gcatgtcacc gttagcgccg cgcattgggc aatactggct gcaactgcct gccacatttg
     5581 gcccacggga tctgcctgcg ctttggcatt ttctcgattc ccttcccggc gaatttaatt
     5641 atggtgtgga agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta
     5701 atcgcggttt acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg
     5761 cagcacgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg
     5821 tacatgctgt actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga
     5881 cgcaaaaccg ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca
     5941 ctacgcctta tcttttttta catacgccag atattgccca ggccccggaa ctggtacata
     6001 ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac
     6061 agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta
     6121 aagggagttt gtatggtaag cgcgctgtat gccgttttaa gtgcgttgtt attaatgaag
     6181 ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg cgacggcggt
     6241 tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc
     6301 gcgattgtgt taatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt
     6361 tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg
     6421 ttccgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg
     6481 gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc
     6541 cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc
     6601 tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat
     6661 gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga
     6721 gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac
     6781 gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc
     6841 tccggcgatg attgaacgct gccgtcgtca tattgacgcc tataaagccc ctacgccagt
     6901 agacgttatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct
     6961 gaattttacc ctgcaattcc tggaaccttc cgagcgtcag gcgttactgg ataaaattta
     7021 tcaagggctg aacccgggcg gtgcgctggt gctttcggaa aaattcagtt tcgaagatgc
     7081 caaagttggt gaattgctgt ttaacatgca ccacgacttt aaacgtgcca acggttacag
     7141 cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctaa ccgattctgt
     7201 ggaaacccat aaagcacgcc tgcatcaagc tggttttgag catagcgagc tgtggttcca
     7261 gtgctttaac tttggttccc tggtggcatt aaaagcagag gacgctgcat gatcgacttt
     7321 ggtaactttt attctctgat tgccaaaaat catctttcac actggctgga aacgctgccc
     7381 gcgcagattg ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac
     7441 gcggtggaat ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtgacg
     7501 gcagaaagcg aagagccact gagcgccggg caaattaaac gtattgaaac gctgatgcgc
     7561 aacctgatgc cgtggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa
     7621 tggcgttccg actggaaatg ggatcgcgtt ctgccccatc tttctgattt aaccgggcgc
     7681 accattcttg atgtcggctg tggcagcggt tatcacatgt ggcgcatgat tggcgcaggc
     7741 gcgcacctcg cggtgggaat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg
     7801 cgtaaactac tgggtaacga tcagcgcgca catttgttac cgttaggcat tgaacagctt
     7861 ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttatca tcgtcgttca
     7921 ccgctggagc atctctggca gttgaaagat caactggtca atgaaggtga actggtgctt
     7981 gaaacgctgg ttattgatgg tgatgagaac acggtgctgg tgccgggcga tcgttacgcg
     8041 caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag
     8101 tgtggttttg ttgatattcg cattgcggat gtgagcgtta ccactacaga agagcagcga
     8161 cgcactgaat ggatggttac cgagtctctg tccgatttcc tcgacccaca tgatccgagt
     8221 aagacagttg aaggttatcc ggcacctaaa cgcgcggtgc tgattgcgcg caagccgtaa
     8281 agattagcgc ccgtttccgg gcgcttttca cgtcttatgc ctgaacagct ggctgatcaa
     8341 acgccgttaa cttcggtgca ttgcccgtat atttttcgat atacaccagc gcagagttac
     8401 cggcacaggc atttgccagc tgagagctgg ggatatccgc cgttaacacg ttcgcactgc
     8461 cgtttttaca caagccattt tccagatctg gccatgcacc ttcatgcacg cataccacgc
     8521 cttttttgat cccgtcagtc accaccgcgc ctgtcagaat ctgaccacgt ctgttccaga
     8581 cacgcaccag atcgccattc gcaataccaa agcgagcagc atcttcggtg tgaatagtga
     8641 tcggttcacg atcggcaacc gcatattttt tacgcagttc cgcatagtta agctgactgt
     8701 gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg gcggaacctt
     8761 tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt
     8821 ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca tttttcaccg
     8881 gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg cggcgcattt
     8941 cgatcagttt attttgctgc cagaaagcat taaacatcgg catagtcacg cgttgcgcac
     9001 gcgcaccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca tctctacctt
     9061 cggtatagat ctcttttccg ccaggtttga gtaattcagc aagatcggca aacacgtcaa
     9121 aatcgttacg cgcttcaaat tgcgcagcga cagcctgctt catcggtaca atatgctggt
     9181 tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg atcggtaata
     9241 cgatatctgc atgtttagca gctgcggtcc agtagcattc agaaacgacg atcatctccg
     9301 gtttctgcca tgctttaatc agacgattag tatcctggtg atgggtaaag ttaccgccac
     9361 ccgcccacca gatcattttg atatttgggt acgtttgctc tttaccgtta tgctgatatt
     9421 tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc gtcattccgc
     9481 catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc ggcagcacgc
     9541 cgccaacacg cgtcgggttg ccaccattgg agtaatgata agagaaacca aacccgccgc
     9601 ccggtgtacc aatctgccct aacatcgccg ccagcgtcac cagcatccag tgtttctgtt
     9661 caccatattg ctggcgctga attccccagc ctgccatcag tattgtgcga tttgccgcca
     9721 tcagttcagc gagtttgact atctgcgctt ccggtacgcc agaaatttcc gccgcccagg
     9781 ccgcgctctt cggcgtatta tcgctcttgc ctgccagata ctcttcaaac tgcggatatc
     9841 cggtagtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg
     9901 cgatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag gtggcattat
     9961 cgccaaagaa ttcgatggtt tcggagcgaa taggatcaat ggcaatcacg ggtttgccag
    10021 attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt
    10081 tcaaggtatt cagcgggttc atcccccaca gcaccacaac ctggctgttt tccagaatca
    10141 gcggccagga ggtctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca
    10201 cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc gcaaggttca
    10261 tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagatcgc cagccgtaag
    10321 atccggcaaa aatggccgat ggtccgttag ctttacgaat acgatcgtgt tgctcatgaa
    10381 tcagttttaa tgcttgttcc cagctcacct gtacataggt atcttcgcca cgacctttcg
    10441 ccggttgcag tggattatcg agatagcttt ttctcaccat cggatgctga atacgcgccg
    10501 tggtgtgtac ctgatccacc gccgtagact gtaaggagtt cggtatagtt ttcgccagcg
    10561 cgcctgttga agaaacaatc ttgccgtcct tcacttctac gttcatcgct ccccaacggc
    10621 ccgcggtgag gattttgcca cccttctctt ctgcccatgc gggtagcggt gcagccgatg
    10681 tcaccaccag cgccccagcg gcaataccgc tgtgtttaat aaattcacgt cttgttaatg
    10741 tcataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct ttggcgttgt
    10801 actggaagta ccgcgtcaaa atgtccagtt cgttttcgct catgctggtt cgcgccccca
    10861 ttcctttggc aatggacggc catgcattga cggtgtaatg gtcggaggca ataggggcat
    10921 ggcaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc ggtttacggt
    10981 ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat tgattgccgt
    11041 attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct tcacttaatg
    11101 tggctagtgc cagccgttga cctgccgcca ggtagagcgt gttttcactg ccctgcattt
    11161 gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg atgagatcgg
    11221 taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga taaatatgta
    11281 cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg ttgtcatcca
    11341 tttttatttc tggcggaaaa tgagcaatgc ctttatgaca atcgatacag gtttcgccgc
    11401 ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca atatccatgg
    11461 catcaaaaga atggcaacta cggcacgttg cagagtcagt ggcttttaat tctttccata
    11521 ctgtttcggc catttcctga cgatgcgctt cgaacttatc gtcactgtct attttgccgc
    11581 taacaaattc atgataaata tctttagatg cttttaattt agcaaataaa taatccatcc
    11641 ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg ttcgaaaagt
    11701 ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg
    11761 ctgtatccga cgttttatgt aaggcttttt gcgccagcaa taacccgccg ccaccaacca
    11821 caaccgctat cagcaaaaac attaacccaa tgcgtttttt ccctcgcata gattcctcta
    11881 attaattcca gaacaccgaa atcattgtat aattaaatat acaacgaatt gaccgcttgc
    11941 ataattcggc ac
//
LOCUS       DAOTRK010000140        11736 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.141, whole genome shotgun sequence.
ACCESSION   DAOTRK010000140 DAOTRK010000000
VERSION     DAOTRK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.141"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1117
                     /locus_tag="QIE41_003771"
     CDS             <1..1117
                     /locus_tag="QIE41_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDS3532607.1"
                     /translation="CALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGMAVAVIY
                     QGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPATKY
                     WPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLY
                     ANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWGYREGKA
                     VHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYWQTGDMY
                     QGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPAPAVRASWVHKTGATGGFGS
                     YVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1205..1738
                     /gene="blc"
                     /locus_tag="QIE41_003772"
     CDS             1205..1738
                     /gene="blc"
                     /locus_tag="QIE41_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDS3532608.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDTKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGDPRRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAIAT
                     REGFDISKFIWVQQPGS"
     gene            complement(1735..2052)
                     /gene="sugE"
                     /locus_tag="QIE41_003773"
     CDS             complement(1735..2052)
                     /gene="sugE"
                     /locus_tag="QIE41_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDS3532609.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2227..2373)
                     /gene="ecnB"
                     /locus_tag="QIE41_003774"
     CDS             complement(2227..2373)
                     /gene="ecnB"
                     /locus_tag="QIE41_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDS3532610.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2484..2609)
                     /gene="ecnA"
                     /locus_tag="QIE41_003775"
     CDS             complement(2484..2609)
                     /gene="ecnA"
                     /locus_tag="QIE41_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDS3532611.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2661..3227)
                     /gene="efp"
                     /locus_tag="QIE41_003776"
     CDS             complement(2661..3227)
                     /gene="efp"
                     /locus_tag="QIE41_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDS3532612.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3269..4297
                     /gene="epmB"
                     /locus_tag="QIE41_003777"
     CDS             3269..4297
                     /gene="epmB"
                     /locus_tag="QIE41_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDS3532613.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4532..5401
                     /locus_tag="QIE41_003778"
     CDS             4532..5401
                     /locus_tag="QIE41_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDS3532614.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(5451..5804)
                     /locus_tag="QIE41_003779"
     CDS             complement(5451..5804)
                     /locus_tag="QIE41_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDS3532615.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(5942..7588)
                     /gene="groL"
                     /locus_tag="QIE41_003780"
     CDS             complement(5942..7588)
                     /gene="groL"
                     /locus_tag="QIE41_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDS3532616.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(7632..7925)
                     /locus_tag="QIE41_003781"
     CDS             complement(7632..7925)
                     /locus_tag="QIE41_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDS3532617.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8201..9457
                     /gene="yjeH"
                     /locus_tag="QIE41_003782"
     CDS             8201..9457
                     /gene="yjeH"
                     /locus_tag="QIE41_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDS3532618.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALGINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(9473..9949)
                     /gene="fxsA"
                     /locus_tag="QIE41_003783"
     CDS             complement(9473..9949)
                     /gene="fxsA"
                     /locus_tag="QIE41_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDS3532619.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            10286..11722
                     /gene="aspA"
                     /locus_tag="QIE41_003784"
     CDS             10286..11722
                     /gene="aspA"
                     /locus_tag="QIE41_003784"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDS3532620.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
BASE COUNT         2953 a         2997 c         2855 g         2931 t
ORIGIN      
        1 ctgcgcctta ttaattaccg cctcttgctc cacatttgct gcccctcaac aaatcaacga
       61 tattgtgcat cgcacaataa ccccgcttat agagcaacaa aagatccccg gtatggcggt
      121 ggcggtaatt tatcagggta aaccttatta ctttacctgg ggctatgcgg acatcgccaa
      181 aaagcagccc gtcacacagc aaacgttgtt tgagttaggc tcggtcagca aaacatttac
      241 gggcgtactt ggtggcgacg ctattgcacg aggggaaatc aagttaagcg atcccgcgac
      301 aaaatactgg cctgaactta ccgctaaaca atggaatggg atcacactat tacatctcgc
      361 aacctacacc gctggcggcc tgccattgca ggtgccggat gaggtgaaat cctcaagcga
      421 cttgctgcgc ttctatcaaa actggcaacc tgcatgggct ccaggaacac aacgtctgta
      481 tgccaactcc agtatcggtt tgttcggcgc actggctgtg aagccgtctg gtttgagttt
      541 tgagcaggcg atgcaaactc gtgtcttcca gccactcaaa ctcactcata cgtggattaa
      601 tgtgccgtcc gcagaagaaa agaattacgc ctggggatat cgcgaaggta aggcagtgca
      661 tgtttcgcca ggggcgttag atgctgaagc ttatggtgtg aagtcgacca ttgaagatat
      721 ggcccgctgg gtgcaaagca atttaaaacc ccttgatatc actgagaaaa cgcttcaaca
      781 agggatacaa ctggcacaat ctcgctactg gcaaaccggc gatatgtatc agggcctggg
      841 ctgggaaatg ctggactggc cggtaaatcc tgacatcatc attaacggca gtgacaataa
      901 aattgcactg gcagcacgcc ccgtaaaagc gattacgccc ccagctcctg cagtgcgcgc
      961 atcatgggta cataaaacgg gggcgaccgg cggatttggt agctatgttg cgtttattcc
     1021 agaaaaagag ctgggtatcg tgatgctggc taacaaaaac tatcccaatc cagcgagagt
     1081 cgccgccgcc tggcagattc tcaacgctct acagtaaaat ttcatcgggt ccgaattttc
     1141 ggaccttttc tgcgcttttc cttgctgtca tctacactta gaaaaaacca gtaaggaaac
     1201 atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg tggttgcctg
     1261 tagttctccc acaccaccgc gtggcgtgac cgtggtaaat aatttcgata ccaaacgcta
     1321 tctgggtaca tggtatgaga ttgcccgttt tgatcaccgt tttgaacgcg ggctggagaa
     1381 agtgaccgcg acgtatagcc tgcgggatga cggcggcctg aatgtcatta ataaaggcta
     1441 taacccggac agagagatgt ggcagcagag tgaaggaaaa gcctacttta ctggagatcc
     1501 gcgtcgcgca gccctgaaag tgtcgttctt tgggccattc tacggcggtt ataatgtcat
     1561 cgcgctcgac cgggagtacc gccacgcgct ggtttgcggc ccggaccgcg actacctgtg
     1621 gatactctct cgcacgccaa ccatttctga cgaagtgaaa caggagatgc tggcaatcgc
     1681 gacccgggaa gggtttgata tcagtaagtt tatttgggta cagcaacccg gtagttaatg
     1741 agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg ccaggcgcat
     1801 cgggttagcg gactcgccga gcagcacaat gccggtgatg gccgcgccga ctgcgccaat
     1861 acccgtccac acggcataag ccgtccctac tggtaacgat ttcatcgccc aggcaagtag
     1921 cgccatactg acaatcatcg ccgtcacagt aataacactt ggcgtcaaac gactaaagcc
     1981 gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa taactaagat
     2041 aatccaggac atatcaggct ccggagaatt ggggccgtcc ccggtgaaaa gaggcgtttg
     2101 caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca ggggaatttc
     2161 aactattacc tttgtgacta tcgcaaaaaa acaaacggca cgacataact gtcgtaccgt
     2221 tattgcttac tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat
     2281 gtcttcacca acgccacgcg tggtgttgca ggcagttaat actgttgaaa gcaccagaac
     2341 agaaaagatc gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt
     2401 tatgtttgcc tatagcaaat taataataga caacatcacc aaaaatgatg gaaatctgat
     2461 gatttttcgg aagagaagaa aaattagctg gcagcgcggg agatggaatt accgagatgt
     2521 ttaatgtctt caccaaaacc gcgagcggta ttacagcccg tgagcagcgc actggcaagc
     2581 aaaacaagaa cgataaggcg tttcatcatc tctggcctga ataagtgatg gtgcagcctg
     2641 caggccgcac cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc
     2701 actttgatga cttcgccgat ctgcacgaac agcggaactt taaccacagc gccagtagac
     2761 agggtagccg gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatcc
     2821 acgatttcca gttcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga
     2881 gttacgatac actctgcctg atccagcagc catttcgcgt tgtcgccaat tgcttttgca
     2941 tcagcagaca gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg
     3001 tacaggtaag tcaggttcat atcgacaaca tcagcgcctt cagcggaatc agtagatttg
     3061 aaggtttttt ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc
     3121 tggcctttac ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac
     3181 atgattttaa gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa
     3241 atttgttaat tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc
     3301 cagagaagat tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg
     3361 tcttttgaat atagacgcgg acgaaaaact gttggccgga cgcagcgcca aaaagctttt
     3421 tgccctgcgt gtgccccgct catttatcga tcgcatggag aaaggcaatc cggacgatcc
     3481 tcttttgcgt caggtactta cctcgcaaga tgagtttgtc gtcgcgcccg gattctccac
     3541 cgacccactg gaagaacagc acagcgtagt gcctggtttg ttgcataaat accacaaccg
     3601 ggcgcttttg ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca
     3661 cttcccctat gccgaaaatc agggcaacaa gcgtaactgg caaactgcac ttgagtatgt
     3721 tgctgcgcat ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa
     3781 agatcacgag ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct
     3841 gcggattcac agccgtctgc cgattgtgat cccggcacgc atcacagatg cgctggttga
     3901 acgcttttca cattctacgc tgcaaatctt gctggtgaat cacatcaacc atgccaatga
     3961 gatagatgaa acattccgtc aggcgatggc taagttgcgc cgggtaggcg ttactttgct
     4021 caaccagagc gttctgttac gtggcgtgaa cgataacgca caaacgctgg caaacctgag
     4081 taatgcgttg ttcgatgccg gcgtgatgcc gtattacctg catgtgctcg ataaggtaca
     4141 gggcgcggcg cattttatgg tgagtgatga cgaagcacgg cagattatgc gtgagttgct
     4201 gacactggtg tcgggatatc tggtgcctaa actggcgcga gaaattggcg gcgaacccag
     4261 caaaacgccg ctggatctcc agctacgcca gcagtaactg attaaacagg gaagtttaat
     4321 ctttgcacgt tgcataacct ttaattattc atatacattg ctgttattac tttttatata
     4381 accaacacca ataattaacc tttcttttct gtttatatag agtgagcacg gctatttatg
     4441 ttattttaac aactattact accaagccca tttatctggc ttataaatta aaattaatta
     4501 tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg gtgttaatac
     4561 cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta aagagcctcg
     4621 caataaagaa tcgttgttct ttctgtctgt catggaactg cgcgaccttc tgattgcgct
     4681 ggaaagccgt ctgcatcaaa agcataaact ggatgctgct gcacgccttc agtatgagca
     4741 agctcgcgat aaagtcatta agaaaatggc agaaaatatc ccagaaattc tggttgatga
     4801 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaggg
     4861 tgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcatcaa
     4921 tgaattgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
     4981 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
     5041 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
     5101 cttgttcaac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
     5161 atttagcggc gcagtggtga aaacgcgtga agcaacgccg ggtaaagaag tggaggcgat
     5221 cactcgccga atgcttgatt tcagcccccg actgaaaaaa ctcgcgggtg tgccatgtca
     5281 ggtctatgtt cgcaccgtag cggcaaataa cgcccaaccg ctaacccagg atcaatgctt
     5341 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
     5401 atcccatctg ttttactacg aggctggaag tatccagcct ctggatccca tcagttcggg
     5461 cacttatata cctggccgat aatctggcta tccgtcggga caaaactgga caacatcccc
     5521 tgcgacgggc tgctgatgcc ataaatcacg ttaccgccca ttgcagccgc ctggttgcgc
     5581 agatcgtttg ctgcgccgcg catagaaccg ccctcttcac cgtgttgccc ggaaagccag
     5641 ttgctttgct tacctgtcgc agtaccaatc agctggcact ctgcgcctgg ctgctcgtcc
     5701 acaatgcgta cactctgacc ggcagcactc aattcgttgc tggagctaca acccgccatc
     5761 agtagcgcgg caccgacaat ccctgctaag tattttacgt gcatgttatt ccccataatc
     5821 aatgagctgg acgccactcg cgtcgtccgt gtctgaatct tatactagaa agatgaccaa
     5881 aagaaaaacc cccagacatt tctgcccggg ggtttgttta tttctgcgag gtgcagggca
     5941 attacatcat gccgcccatg ccacccatgc cgcccatacc gccagcagcg cctaagtcag
     6001 ctgcatcgtt tttcggcagg tcggtaacca tgcactcggt ggtgatcatc aggccagcca
     6061 cagaagccgc gtactgcaga gcagaacggg ttactttggt tggatccagg atacccatgt
     6121 cgatcatgtt gccgtattct tcggttgctg cgttgtaacc gtagttaccg tcgccgcctt
     6181 taacggtgtt agcaacaaca gacggttctt cgccgcagtt caggacgatc tgacgcagcg
     6241 gagcttccat tgcacgcagt gcaactttga tacccacgtt ctggtcttcg ttctgaccac
     6301 gcaggtcagc cagtttagac gctacgcgga tcagcgcaac accaccacca gcaaccacgc
     6361 cttcttctac cgcagcacgg gtcgcgtgca gggcgtcttc aacgcgtgct tttttctctt
     6421 tcatttcaac ttcggtagca gcacctactt tgataactgc aacgccgcct gccagtttcg
     6481 ctacgcgctc ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca atctgctgac
     6541 ggatctgagc aacacggccc tggattgcag cttcttcacc cacgccatcg atgatggtgg
     6601 tagtgtcttt gttgatcaca acacgtttag cctgacccag gtcttccagg gttgcttttt
     6661 ccagctccat accgatctct tcagagatta cggtaccgcc agtcagggtt gcgatatcct
     6721 gcagcatagc tttacgacga tcgccgaagc ccggagcttt aaccgcagca actttcacga
     6781 tgccacgcat ggtgttaaca accagagttg ccagcgcttc gccttctaca tcttcagcga
     6841 tgatcagcag cggtttgcct gctttggcaa cagcttccag aaccggcagc atttcgcgga
     6901 tgttggagat tttcttgtca gccagcagga tgaacgggct ttccagttct actgcgccag
     6961 tttccggctt gttgatgaag taaggagaca ggtagccacg gtcgaactgc ataccttcaa
     7021 ccacgtccag ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg ccttctttac
     7081 cgactttgtc catcgcttca gcgatcagtt tacctacggt ttcgtcggag ttagcggaga
     7141 tggtaccaac ctgagcaatc gctttagagt cagagcacgg tacggacagc gctttcagtt
     7201 cttcaactgc agcggtaaca gctttgtcga taccacgttt caggtccatc gggttcatgc
     7261 ccgcagcaac agctttcaga ccttcagtga tgatagcctg agccagtacg gttgcagtgg
     7321 tggtaccgtc gcctgcagcg tcgttcgctt tagaggcaac ttctttcacc atctgcgcac
     7381 ccatgttttc gaacttatct tccagttcga tttcacgagc aacggaaaca ccatctttgg
     7441 tgatggtcgg tgcaccgaaa gatttatcca gaactacgtt acggcctttc ggaccgaggg
     7501 taactttcac tgcatctgcc agtacgttta cgccgcgcag cattttcaca cgagcgtcgt
     7561 taccgaattt tacgtcttta gctgccatta tctttattcc ttaaattcgt atgttcagtg
     7621 tcgtgcgcgg attacgcttc aacaattgcc agaatgtcgc tttcggacat gatcaacact
     7681 tcttcattgt cgatcttctc agatttcaca ccgtagccat cgttgaaaat aacgatgtcg
     7741 ccaactttca catccagcgg cttcacttcg ccattttcaa ggatacggcc attgccgaca
     7801 gccagcactt cgccgcgggt ggatttagcc gctgcagagc cggtcagaac gatgccgcca
     7861 gcagatttag tttcaacttc tttacgcttg acgatcacgc gatcatgcaa tggacgaata
     7921 ttcattgata actctccttt gagaaagtcc gtatctgtta tgggtgacgc cggagcttac
     7981 gtggtttccc ggctggtgac cagagaaatg gggatgaggc ttcgcccctt caagggggaa
     8041 aaagaaaaaa aattctgatc agcacaaaat cgggtgaaaa ccctgattca cctcacattt
     8101 catcgcaatt tcttcatcac cgtcataagc gaatctgatt gtgctaccat cgaaaatcta
     8161 cgcagttgcc cataatttgg gcgcaatcgg acatcaaccc atgagtggac tcaaacaaga
     8221 actgggactg gcccagggca ttggcctgct atcgacgtca ttattaggca ctggcgtgtt
     8281 tgccgttcct gcgttagctg cgctggtagc gggcaataac agcctgtggg cgtggcccgt
     8341 tttgattatc ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc gccactatcc
     8401 cagcgcaggc ggcgtcgcgc acttcgtcgg tatggcgttt ggttcgcggc ttgagcgagt
     8461 caccggctgg ctgtttttat cagtcattcc cgtgggtttg cctgccgcac tacaaattgc
     8521 agccggattc ggccaggcaa tgtttggctg gcatagctgg caactgttgt tggcagaact
     8581 cggtacgctg gcgctggtgt ggtatatcgg tactcgcggt gccagttcca gtgctaatct
     8641 acaaacagtt attgccggac ttatcgtcgc gctgattgtc gctatctggt gggcgggcga
     8701 tatcaaacct gcgaatatcc ccttccctgc acctggtaat atcgaactta ccgggttatt
     8761 tgctgcgtta tcagtgatgt tctggtgctt tgtcggtctg gaagcatttg cccatcttgc
     8821 ctcggaattt aaaaatccag agcgtgattt tcctcgtgct ttgatgattg gtctgctgct
     8881 ggcaggatta gtctactggg gctgtacggt agtcgtctta cacttcgacg cctatggtga
     8941 acaaatggcg gcagcagcat cgcttccaaa aattgtagtg caactgttcg gtgtaggagc
     9001 gttatggatt gcctgcgtga ttggctatct ggcctgcttt gccagtctca acatttatat
     9061 acagagcttc gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc actacctggc
     9121 acgcctctct tctcgtcata tcccgaataa tgccctcaat gcggtgctcg gctgctgcgt
     9181 ggtgagcacg ttggtgattc atgctttagg gatcaatctg gacgcactta tcatctatgc
     9241 caatggcatc tttattatga tttatctgtt atgcatgctg gcaggctgta aattattgca
     9301 aggacgttat cgactactgg cggtggttgg cgggctgtta tgcgttctgt tactggcaat
     9361 ggtcggctgg aaaagtctct atgcgctgat catgctggcg gggttatggc tgtttctgcc
     9421 aaaacgaaaa acgccggaaa atggcataac cacataatcc ggcgtttcgg cattaatcct
     9481 ggcgatcgtc tttatgatca aggcggtcgc ggtcatcatc ctttcgctgg tactcaccat
     9541 caaaagtatt accgccaccg gtcccggcgc taaaaccgcc gcctggcatg cgagaaaagc
     9601 gcaaatgcgg catcaacttc actgtcagat gcttttgcac cggcggtaat aaaagcagaa
     9661 gaccgaggaa gtcggtgaaa aagcccggta ataaaagcag caaaccggca atgatcagcg
     9721 aaacactttt aatcatctcc gccgctgggt tttcacccgc cgccattttt tgctgcatca
     9781 gcacgaaatt cttaaagccc tggttacgta ccagtgacat accgataact gacgtgaata
     9841 taaccagcac gagggtcagc aataccccca atacatgggc aacctgaata aagattgaaa
     9901 tctcaatata gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa ggtttctcct
     9961 gtaatagcag ccggttaacc ccggctacct gaatgggttg cgaatcgcgt ttagcttata
    10021 ttgtggtcat tagcaaaatt tcaagatgtt tgcgcaacta tttttggtag taatcccaaa
    10081 gcggtgatct atttcacaaa ttaataatta aggggtaaaa accgacactt aaagtgatcc
    10141 agattacggt agaaatcctc aagcagcata tgatctcggg tattcggtcg atgcagggga
    10201 taatcgtcgg tcgaaaaaca ttcgaaacca catatattct gtgtgtttaa agcaaatcat
    10261 tggcagcttg aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt
    10321 gggtaccagg gaagttccag ctgatgccta ctatggtgtt cacactctga gagcgattga
    10381 aaacttctat atcagcaaca acaaaatcag tgatattcct gaatttgttc gcggtatggt
    10441 aatggttaaa aaagccgcag ctatggcaaa caaagagctg caaaccattc ctaaaagtgt
    10501 agcgaatgcc atcattgccg catgtgatga agtcctgaac aacggaaaat gcatggatca
    10561 gttcccggta gacgtctacc agggcggcgc aggtacttcc gtaaacatga acaccaacga
    10621 agtgctggcc aatatcggtc tggaactgat gggtcaccag aaaggtgaat atcagtacct
    10681 gaacccgaac gaccatgtta acaaatgtca gtccactaac gacgcctacc cgaccggttt
    10741 ccgtatcgca gtttactctt ccctgattaa gctggtagat gcgattaacc aactgcgtga
    10801 aggctttgaa cgtaaagctg tcgaattcca ggacatcctg aaaatgggtc gtacccagct
    10861 gcaggacgca gtaccgatga ccctcggtca ggaattccgc gctttcagca tcctgctgaa
    10921 agaagaagtg aaaaatatcc aacgtaccgc tgaactgctg ctggaagtta accttggcgc
    10981 aacagcaatc ggtactggtc tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa
    11041 actggctgaa gtcactggct tcccatgcgt accggctgaa gacctgatcg aagcgacctc
    11101 tgactgcggc gcttatgtta tggttcacgg cgcgctgaaa cgcctggctg tgaagatgtc
    11161 caaaatctgt aacgacctgc gcttgctctc ttctggccca cgtgccggcc tgaacgagat
    11221 caacctgccg gaactgcagg cgggctcttc catcatgcca gctaaagtaa acccggttgt
    11281 tccggaagtg gttaaccagg tatgcttcaa agtcatcggt aacgacacca ctgttaccat
    11341 ggcagcagaa gcaggtcagc tgcagttgaa cgttatggag ccggtcattg gccaggccat
    11401 gtttgaatcc gttcacattc tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa
    11461 cggcatcact gctaacaaag aagtgtgcga aggttacgtt tacaactcta tcggtatcgt
    11521 tacttacctg aacccgttca tcggtcacca caacggtgac atcgtgggta aaatctgtgc
    11581 cgaaaccggt aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga
    11641 acttgacgat attttctccg tacagaatct gatgcacccg gcttacaaag caaaacgcta
    11701 tactgatgaa agcgaacagt aatcgtacag ggtagt
//
LOCUS       DAOTRK010000141        11721 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.142, whole genome shotgun sequence.
ACCESSION   DAOTRK010000141 DAOTRK010000000
VERSION     DAOTRK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.142"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            47..946
                     /gene="hisG"
                     /locus_tag="QIE41_003785"
     CDS             47..946
                     /gene="hisG"
                     /locus_tag="QIE41_003785"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDS3532621.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            952..2256
                     /gene="hisD"
                     /locus_tag="QIE41_003786"
     CDS             952..2256
                     /gene="hisD"
                     /locus_tag="QIE41_003786"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDS3532622.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVAISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            2253..3323
                     /gene="hisC"
                     /locus_tag="QIE41_003787"
     CDS             2253..3323
                     /gene="hisC"
                     /locus_tag="QIE41_003787"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDS3532623.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKAEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLKNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLMAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3323..4390
                     /gene="hisB"
                     /locus_tag="QIE41_003788"
     CDS             3323..4390
                     /gene="hisB"
                     /locus_tag="QIE41_003788"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDS3532624.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4390..4980
                     /gene="hisH"
                     /locus_tag="QIE41_003789"
     CDS             4390..4980
                     /gene="hisH"
                     /locus_tag="QIE41_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDS3532625.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGIDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            4980..5717
                     /gene="hisA"
                     /locus_tag="QIE41_003790"
     CDS             4980..5717
                     /gene="hisA"
                     /locus_tag="QIE41_003790"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDS3532626.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5699..6475
                     /gene="hisF"
                     /locus_tag="QIE41_003791"
     CDS             5699..6475
                     /gene="hisF"
                     /locus_tag="QIE41_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDS3532627.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6469..7080
                     /locus_tag="QIE41_003792"
     CDS             6469..7080
                     /locus_tag="QIE41_003792"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDS3532628.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
     gene            complement(7176..8156)
                     /gene="wzzB"
                     /locus_tag="QIE41_003793"
     CDS             complement(7176..8156)
                     /gene="wzzB"
                     /locus_tag="QIE41_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDS3532629.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8302..9468)
                     /gene="ugd"
                     /locus_tag="QIE41_003794"
     CDS             complement(8302..9468)
                     /gene="ugd"
                     /locus_tag="QIE41_003794"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDS3532630.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(9716..11122)
                     /gene="gndA"
                     /locus_tag="QIE41_003795"
     CDS             complement(9716..11122)
                     /gene="gndA"
                     /locus_tag="QIE41_003795"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDS3532631.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKDEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLDYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11227..>11721)
                     /locus_tag="QIE41_003796"
     CDS             complement(11227..>11721)
                     /locus_tag="QIE41_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS3532632.1"
                     /translation="DYYLSVCRIEPENNVELILKTFSKLKYKIKFIGNWNGSEFGKKL
                     RLHYSNYPNIEMIDPIYDLQQLFHLRNNCIGYIHGHSAGGTNPSLVEAMHFSKPIFAY
                     DCKFNRYTTENEACYFSNESDLAEKIIMHCELSLGVSGTKMKEIANQKYTWRRIAEMY
                     EDCY"
BASE COUNT         2931 a         2956 c         3006 g         2828 t
ORIGIN      
        1 tgcgcggttg ataacggttc agacaggttt aaagaggaat aacaaaatga cagacaacac
       61 tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgattcac gcgaattgct
      121 ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa
      181 catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg taatggatgg
      241 cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg
      301 cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggattttg gcggctgccg
      361 actttcactg gcaacgccgg ttgatgaagc ctgggacggc ccgctctcct taaacggtaa
      421 acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga aaggcatctc
      481 ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg gactggcgga
      541 tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gcaaacggcc tgcgcgaagt
      601 cgaagttatc taccgctcga aagcttgcct gatccaacgc gatggcgaaa tggaagaatc
      661 caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgtgatcc aggcgcgcga
      721 atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca tcgccctgct
      781 gccaggtgcc gaacgcccaa ctattctgcc gctggcgggt gatcaacagc gcgtagcgat
      841 gcacatggtc agcagcgaaa ccctgttctg ggaaaccatg gaaaaactga aagcgctggg
      901 tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc catgagcttt
      961 aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct gttaatgcgc
     1021 ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct cgataacgtg
     1081 aaagcacgcg gtgatgacgc cctgcgggaa tacagcgcga agtttgataa aaccacggtt
     1141 accgcactga aggtgtctgc tgaggaaatt gccgccgcca gcgaacgcct gagcgacgag
     1201 ctaaaacagg cgatggcggt ggcagtaaag aatattgaaa ccttccacac tgcgcaaaaa
     1261 ctgccgccgg tagatgttga aactcagcct ggtgtgcgtt gccagcaggt cacgcgcccg
     1321 gtagcttcgg ttggtttgta tattcctggc ggttccgccc cgctcttctc aacggtatta
     1381 atgctggcga ctccggcgcg tattgcgggt tgcaataaag tggtgctgtg ctcaccgccg
     1441 ccgattgccg atgaaattct ttacgcggcg cagctatgcg gtgtgcagga cgtgtttaac
     1501 gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt gccgaaagtg
     1561 gacaaaatct tcgggccggg taacgccttt gtcaccgaag cgaaacgtca ggtgagccag
     1621 cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct ggtgattgct
     1681 gacagcggcg ctacgccgga tttcgtggct tctgatttgc tttctcaggc tgaacacggc
     1741 ccggattcac aggtgatttt actgacgcct gacgctgata tggcgcatca agttgccgaa
     1801 gccgtcgaac gccagttagc agaactgccg cgtgccgaaa ccgcacgtca ggcactgagc
     1861 gccagccgcc tgatcgtgac caacgattta gcgcagtgcg tggcaatctc caaccagtac
     1921 ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga tagcatcacc
     1981 agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggtga ttacgcctcc
     2041 ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag cctcggactg
     2101 gcggatttcc agaagcggat gaccgtgcag gaactgtcga aagtaggttt ctccgcgctg
     2161 gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa aaatgccgtt
     2221 actttgcgtg ttaacgccct taaggagcaa gcatgagcac cgtgactatt accgatttag
     2281 cgcgtgagaa cgtccgcaac ctgacgccgt atcagtcggc gcgtcgtctg ggcggtaacg
     2341 gcgacgtctg gctgaacgcc aacgaatacc ccacagccgt ggagtttcag cttactcagc
     2401 aaacgctcaa ccgctacccg gaatgccagc cgaaagcggt gattgaaaat tacgcgcagt
     2461 atgcaggcgt gaaggcggag caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac
     2521 tgctgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc ccgccaacgt
     2581 acggcatgta cagcgtcagc gccgaaacca ttggcgtcga gtgccgcaca gtgccgacgc
     2641 tgaaaaactg gcaactggac ttgcagggca tttccgacaa gctggacggc gtaaaagtgg
     2701 tttatgtttg cagccccaac aacccgaccg ggcaactgat caatccacag gattttcgca
     2761 ccctgctgga gttaacgcgc ggtaaagcga ttgtggttgc cgatgaggcc tatatcgagt
     2821 tttgcccgca ggcatcgctg gctggctggc tggcggaata tccgcacctg gctattttgc
     2881 gcacactgtc gaaagccttc gctctggcgg gccttcgttg cggatttacg ctggcaaacg
     2941 aagaagtcat caacctgctg atgaaagtga tcgcccccta cccgctctcg acgccggttg
     3001 ccgacattgc agcccaggcg ttaagcccgc aggggatcgt cgccatgcgc gaacgagtgg
     3061 cgcaaattat tgctgaacgc gaatacctga tggccgcact gaaagagatc ccctgcgtgg
     3121 agcaggtttt cgactccgaa accaactaca ttctggcgcg ctttaaagcc tccagcgcag
     3181 tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa caaccctctt
     3241 taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc gtcattgacg
     3301 ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc gcgatggaac
     3361 cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac tcgcctttga
     3421 accgggcgtg atcccggaac tgctgaagct gcaaaaagcg ggctacaaac tggtgatgat
     3481 cactaatcag gatggtctgg gaacacaaag tttcccgcag gcggattttg atggaccgca
     3541 caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag tgctgatttg
     3601 tccgcacctg cccgcagatg agtgcgactg ccgtaaaccg aaagtaaaac tggtggagcg
     3661 ttatctcgct gagcaagcga tggatcgtgc caacagttat gtgattggcg atcgcgcgac
     3721 cgatattcaa ctggcggaaa acatgggtat taatggttta cgctacgacc gcgaaaccct
     3781 gaactggccg atgattggcg agcaactcac taaacgagac cgttacgccc acgtagtgcg
     3841 caacaccaaa gagacgcaaa ttgacgtcca ggtgtggctg gatcgtgaag gtggcagcaa
     3901 gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca cccacggcgg
     3961 tttccgcatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc acaccgtcga
     4021 agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgata aacgcggtat
     4081 ttgtcgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg cgctggatat
     4141 ctctggtcgc ccgcacctgg aatataaagc cgaatttacc taccagcgcg tgggcgatct
     4201 cagcactgaa atgatcgagc acttcttccg ttcgctctct tacactatgg gcgtgacgct
     4261 acacctgaaa accaaaggta aaaacgatca tcaccgtgta gagagcctgt tcaaagcctt
     4321 tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgac accctgccct cgtcaaaagg
     4381 agtgctgtaa tgaacgtggt gatccttgat accggctgcg ccaatctgaa ctcggtgaag
     4441 tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga cgtcgtgttg
     4501 ctggccgata aactgttttt acccggcgtt ggcaccgcgc aagcggcgat ggaccaggtg
     4561 cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct gggcatctgc
     4621 ttagggatgc aactgctggg gcggcgcagc gaagagagca acggcatcga cttgctgggc
     4681 atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccgctgcc gcatatgggc
     4741 tggaaccgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga agacggcgcg
     4801 tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat cgcccagtgt
     4861 aactacggcg aaccgttcac cgcggcggta caaaaagata acttctatgg cgtgcagttc
     4921 cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct ggagatgtga
     4981 tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat cagggcgatt
     5041 atggcaaaca acgcgattac ggtaacgacc cgctgccacg cttacaggat tacgccgcac
     5101 aaggtgctga agtgctgcac ctggtggatc tgaccggggc aaaagatccg gctaaacgtc
     5161 aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt tccggtgcag gttggcggcg
     5221 gcgtgcgtag cgaagaagac gtagcggcgt tactggaagc aggtgttgcg cgtgtggtag
     5281 ttggctccac cgcggtgaaa tcaccagaaa gggtgaaagg ctggtttgaa cgcttcggtg
     5341 cggatgcctt agtgctggcg ctggatgtcc gtattgacga gcaaggcaac aagcaagtgg
     5401 cggtcagtgg ctggcaagag aattcgggtg tgtcgctgga acaactggtg gaaacctatc
     5461 tgcccgtcgg cctgaaacat gtgctgtgta ccgatatctc gcgcgacggc acgctggcag
     5521 gctctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaagta gcatttcagt
     5581 cctccggcgg tattggcgac attaatgatg tcgctgccct gcgtggcact ggcgtgcgcg
     5641 gcgtaatagt tggtcgggca ttactggaag gtaaattcac cgtgaaggag gccatcgcat
     5701 gctggcaaaa cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt
     5761 acagtttcgc aaccatgaaa tcattggcga tatcgtgccg ctagcaaaac gctacgccga
     5821 agaaggtgcg gacgagctgg tgttctacga tatcaccgct tccagcgatg gacgggtggt
     5881 agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt gtgtggcggg
     5941 tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg ataaaatttc
     6001 catcaactct cctgcgctgg cggacccgac gttaattact cgcctggccg atcgctttgg
     6061 cgtgcagtgt attgtggtcg gtattgatac ctggtacgac gccgaaaccg gtaaatatca
     6121 tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg aaacgctcga
     6181 ctgggtacag gaagtgcaaa aacgcggtgc cggagaaatc gtcctcaata tgatgaatca
     6241 ggacggcgtg cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg aagtttgcca
     6301 cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctcg aagccttccg
     6361 cgatgccgac gttgacggcg cgctggcagc ttccgtattc cacaaacaaa taatcaatat
     6421 tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat gttaacagaa
     6481 caacaacgtc gcgaactgga ctgggaaaaa accgacggac ttatgccggt gattgtgcaa
     6541 cacgcggtat ccggcgaagt gctaatgctg ggctatatga acccggaagc cttagacaaa
     6601 accctcgaaa gcggcaaagt caccttcttc tcgcgcacta aacagcgact gtggaccaaa
     6661 ggcgaaacgt cgggcaattt cctcaacgta gtgagtattg ccccggactg cgacaacgac
     6721 acgttactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac cagcagctgc
     6781 ttcggcgaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact gctcgccgaa
     6841 cgtaaatctg ccgacccgga aacctcctac actgccaaac tgtatgccag cggcaccaaa
     6901 cgcattgcgc agaaagtagg cgaagaaggc gtggaaaccg cgctggccgc gacggtacat
     6961 gaccgctttg agctgaccaa cgaggcatct gatttgatgt atcacctgct ggtgttgttg
     7021 caggatcagg ggctggattt aacgacggta attgagaacc tgcgtaaacg gcatcagtga
     7081 gttgcggggt aagcggatgc gatattgttg ccgcatccgg caaaaaaacg ggcaaggtgt
     7141 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttgta attgcgtaga
     7201 gcattacgcc ccagcacaat ccccgcgcca accatgccac ccagcagcac cgccagaatc
     7261 aaggtaattg cctttttcgg gctatcacga cgaataggta acgtcggttt cattacatag
     7321 cggtaagcat gaatatcaag atcatcaacc tttaagcttt cgatatcaag caggttttga
     7381 cgagtctgat agtagtttgg tgagaacacc aacggacggg tcgcctcatg cttaatcatc
     7441 gactccagcg cttcgctccc caaaaggaac aacgtatctt gtgtgatatc ttcgccagtc
     7501 tgttgaatct gcggttttgt cacctgcgcc tgattcgcat actgcaacgc ttcctgaatc
     7561 tgacggatac gcagatcttt ctgctcctgc gcaaccactt cctgcgttct taaagagtcc
     7621 tgcaagtttt tccgtcccag agcaatgttg tccttgagat ccttttctaa ctcttgattc
     7681 actttatcgt caacttgctg aatgtattgg gccaacttca tttgtgcgcc ctctgcagtt
     7741 tgcccaacat aagaaacagt caatggtaat tgctggttct taacagaagg ttcgatggta
     7801 agtttttctg gttcttcctg attatccagc gtttctgcta atgcagagaa ggcagaactg
     7861 aagcgaccaa ttaacgtctc ctgcaaatcc gatactttcg gtgcagcctg accatagata
     7921 acattcatgg cattgttata gccagcaatt tgccccacat cgggctgagt gataattgct
     7981 gttgacgtcc atttctcctt cgctactgcc aaatatccaa tagccagggc aatagccaca
     8041 atgacggaaa tgatgattgt catcttgcca cgccacaact gcactagtaa atcaatcaaa
     8101 tcaatctgtt ccgggtcatg gttttgccca gaaacattat tattttctac tctcatcatt
     8161 accctaactg acggaaattt ccaagtccgc atagtttagc gataatcctc atgaatgcta
     8221 taggataatt gataaaaata atgaattgtt acgcaagtaa caaaagacaa tcagggcgta
     8281 aatagccctg ataacaggat gttagtcgct gccaaagaga tcgcgggtgt agaccttatc
     8341 tgccacatct ttaagctctt ctgccatacg gttggaaata atgacatcgg cttgttgttt
     8401 gaaagtggcg agatcacgtt ccaggcgaga gttgaagaat gaatcttctt tcatcaccgg
     8461 ttcgtagatg atcacttcaa cgcctttcgc cttgatacgt ttcataatcc cctgaatgga
     8521 agacgcacgg aagttatctg acccgctctt cataatcaga cgataaatac ccaccacttg
     8581 cggtttgcgt gacaaaatgg catcggcaat aaagtcttta cgtgtgcggt tagcatcgac
     8641 aattgccgag atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc
     8701 tttcggcaga caataaccac catagccaaa cgacgggttg ttgtaatggt tgccaatacg
     8761 cggatcgaga caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata
     8821 gctatccagt tcgttaaagt acgccacgcg catcgccagg taggtgtttg caaaaagttt
     8881 aatcgcttct gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgctcc
     8941 ttcctgtaac agagcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg
     9001 tgaaggatgg agattatcgt aaagggcttt accctcacgg agaaattccg gggagaatat
     9061 aatattttca gtgcgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga
     9121 tttgatcacc ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac
     9181 actggatgta ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac
     9241 ataatcagca tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc
     9301 tgattgcaaa aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat
     9361 agcaacgcgt gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag
     9421 cccgtttgac aagccgacat agccagtacc ggaaatggtg attttcattt catgctctca
     9481 gaattaactt aactgtgaat catgatgttt ttagcatcct gataagagtt aaaagtttta
     9541 acgctacgtt gtaagatggt caacgcaacg aaaaacaatt cagaatttct ttataagaat
     9601 agctgatatt tattaagtta atattaagca gtgaaattta gtctataaga taattggtaa
     9661 aaaaaacccg gcataatata cgccgggcct caattttatt gttgattaaa tcagattaat
     9721 ccagccattc ggtatggaac acaccttctt tatcaatgcg cttataagta tgcgcaccga
     9781 aatagtcacg ctgtgcctgg atcaggttcg caggcagaac agcggcgcgg tagctgtcat
     9841 aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtacc gcgtaagcga
     9901 cgacatcgcg cagcgcctgc tggtagtcat cggcgatttg cttgaagtac ggagccagca
     9961 gcaggttagc gatctgcgga ttttcggcat aagcatcggt gattttctgc aggaactgcg
    10021 cacggatgat gcagccagca cggaaaatct tcgcgatttc gccgtagtcc agatcccagt
    10081 tgtactcttc agacgccgca cgcagttgag agaagccctg agcgtaagaa acgattttgc
    10141 ccagatacag tgcacggcga actttttcga tgaactcatc tttatcgcct gctggctgcg
    10201 cttgcgggcc agagagaact ttagatgcgg caacacgctg atctttcaga gaagagatat
    10261 aacgtgcaaa cacagactcg gtaatcagcg acagcggttc accgagatcc agcgcgctct
    10321 ggctggtcca tttaccggta cctttgttag ccgcttcatc cagaatcaca tcaaccaggt
    10381 agttaccgtc ttcatctttt ttagtgaaga tgtctttagt gatgtcaatc aggtagctgc
    10441 tcagttcacc gttattccac tcggtaaagg tctgcgccag ttcttcgttg gaaaggttca
    10501 agccaccttt aagcagagaa taggcttcag caatcagctg catatcgccg tattcaatac
    10561 cgttgtgaac catcttcaca taatggcctg cgccatcggc accaatatag gtaacgcatg
    10621 gctccccgtc ttcagctact gcggcgattt tggtcaggat tggtgcaacc agttcatagg
    10681 cttctttctg cccaccaggc ataatagaag gacctttcag cgcgccttct tcaccaccgg
    10741 agacaccggt accaatgaag ttaaagcctt cggcagaaag ttcacggtta cgacgaatgg
    10801 tgtcctggaa gaaggtattg ccaccatcaa tgatgatgtc acctttatcg agatatggtt
    10861 tgagggaatc aatagcagca tccgtgcctg cacctgcttt caccattaac aggatgcgac
    10921 gaggcgtttc cagagattca acaaactctt tcaccgtata gtaaggaacc agtttcttgc
    10981 ctggattttc ggcaatcact tcttccgtct tttcacggga acggttgaaa atagagacgg
    11041 tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc catcactgcc ataccgacga
    11101 cgccgatctg ttgctttgac attgtttact cctgtcaggg ggtggtcacc gcgagagcgg
    11161 cttgaaaaac aattaatgcg cttatagtca tgccatatat tgtaaaagat ttgaagttta
    11221 acagagttaa tagcaatcct catacatttc tgctattcgt ctccaagtgt atttctggtt
    11281 agcaatttct ttcattttcg tgccagagac acctaatgat agctcacaat gcattatgat
    11341 tttctctgcg aggtcagatt cattagaaaa ataacatgct tcattttcag tagtgtacct
    11401 attaaactta caatcatatg caaatatagg tttactaaaa tgcattgcct cgactaaaga
    11461 agggtttgtt cctccagccg aatgaccatg tatatatcct atgcaattat ttcgtaagtg
    11521 aaataattgt tgaagatcat aaatcggatc aatcatttca atatttggat agttagaata
    11581 atgcagccta agtttctttc caaactcgct gccattccaa tttccaataa attttatttt
    11641 atattttagc tttgaaaatg tttttaaaat taattctaca ttgttttcgg gttcgatacg
    11701 acatacagaa aggtagtaat c
//
LOCUS       DAOTRK010000142        11716 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.143, whole genome shotgun sequence.
ACCESSION   DAOTRK010000142 DAOTRK010000000
VERSION     DAOTRK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.143"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            32..673
                     /gene="pyrE"
                     /locus_tag="QIE41_003797"
     CDS             32..673
                     /gene="pyrE"
                     /locus_tag="QIE41_003797"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDS3532633.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(710..1306)
                     /gene="slmA"
                     /locus_tag="QIE41_003798"
     CDS             complement(710..1306)
                     /gene="slmA"
                     /locus_tag="QIE41_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDS3532634.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(1413..1871)
                     /gene="dut"
                     /locus_tag="QIE41_003799"
     CDS             complement(1413..1871)
                     /gene="dut"
                     /locus_tag="QIE41_003799"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDS3532635.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(1849..3069)
                     /gene="coaBC"
                     /locus_tag="QIE41_003800"
     CDS             complement(1849..3069)
                     /gene="coaBC"
                     /locus_tag="QIE41_003800"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDS3532636.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            3241..3909
                     /gene="radC"
                     /locus_tag="QIE41_003801"
     CDS             3241..3909
                     /gene="radC"
                     /locus_tag="QIE41_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDS3532637.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4126..4362
                     /gene="rpmB"
                     /locus_tag="QIE41_003802"
     CDS             4126..4362
                     /gene="rpmB"
                     /locus_tag="QIE41_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDS3532638.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            4383..4550
                     /gene="rpmG"
                     /locus_tag="QIE41_003803"
     CDS             4383..4550
                     /gene="rpmG"
                     /locus_tag="QIE41_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDS3532639.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            4648..5457
                     /gene="mutM"
                     /locus_tag="QIE41_003804"
     CDS             4648..5457
                     /gene="mutM"
                     /locus_tag="QIE41_003804"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDS3532640.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(5496..5975)
                     /gene="coaD"
                     /locus_tag="QIE41_003805"
     CDS             complement(5496..5975)
                     /gene="coaD"
                     /locus_tag="QIE41_003805"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDS3532641.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(5983..7260)
                     /gene="waaA"
                     /locus_tag="QIE41_003806"
     CDS             complement(5983..7260)
                     /gene="waaA"
                     /locus_tag="QIE41_003806"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDS3532642.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            7673..8731
                     /gene="rfaQ"
                     /locus_tag="QIE41_003807"
     CDS             7673..8731
                     /gene="rfaQ"
                     /locus_tag="QIE41_003807"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDS3532643.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLVCVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            8728..9852
                     /locus_tag="QIE41_003808"
     CDS             8728..9852
                     /locus_tag="QIE41_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDS3532644.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEVE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            9845..10642
                     /gene="rfaP"
                     /locus_tag="QIE41_003809"
     CDS             9845..10642
                     /gene="rfaP"
                     /locus_tag="QIE41_003809"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDS3532645.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            10658..11674
                     /gene="waaO"
                     /locus_tag="QIE41_003810"
                     /gene_synonym="rfaI"
     CDS             10658..11674
                     /gene="waaO"
                     /locus_tag="QIE41_003810"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HDS3532646.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVSYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDAIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
BASE COUNT         2989 a         2770 c         3012 g         2945 t
ORIGIN      
        1 tctgtagaaa agtaagatga ggagcgaagg catgaaacca tatcagcgcc agtttattga
       61 atttgcgctt agcaagcagg tgttaaagtt tggcgagttt acgctgaaat ccgggcgcaa
      121 aagcccctat ttcttcaacg ccgggctgtt taataccggg cgcgatctgg cactgttagg
      181 ccgtttttac gctgaagcat tggtggattc cggcattgag ttcgatctgc tgtttggccc
      241 tgcttacaaa gggatcccga ttgccaccac aactgccgtg gcgctggcgg agcatcatga
      301 cctggacctg ccgtactgct ttaaccgcaa agaagcaaaa gaccacggtg aaggcggcaa
      361 tctggtcggt agcgcgttac aaggacgcgt aatgctggtg gatgatgtga tcaccgccgg
      421 aacggcgatc cgcgagtcga tggaaattat tcaggccaat ggcgcgacgc ttgctggcgt
      481 attgatttcg ctcgatcgtc aggaacgggg gcgcggcgag atttcggcaa ttcaggaagt
      541 tgagcgtgat tacaactgca aagtgatctc tatcatcacc ctgaaagacc tgattgctta
      601 cctggaagag aagccggaaa tggcggaaca tctggcggcg gttaaggcct atcgcgaaga
      661 gttcggcgtt taaagaaact cgccggatga aaagtcatcc ggcgtcatat tactgcaact
      721 gtgcggcaat tagcggccag cgggcgtcaa aatcatccgt cgggcggtat ttaaactcgc
      781 tgcggacaaa gcgtgacagc ataccttcac agaaggccag gatctggctt gccagcaggg
      841 tttcatcggt ggtgtaacct tcaccctcac gcattctctt ttcgcgcaat acctgacgca
      901 actgcgcttc aatacgctcg aacagctggt tgatgcgccc ttgcaggcga tcctgttcaa
      961 acattagtgc atgaccagtg aggatgcggg tcaggccagg attacgctca ccaaaaccga
     1021 gaagcagcaa cacaatcaga cgcaggcgcg ctgtggtgtc cttctcatct ttcagaatca
     1081 gattgatgcg agtaatcagg ctatcttcga taaactcaat caggctatcg aacatgcggg
     1141 tcttactggg gaagtggcga tacagtgccg cttcggaaac gccgacagag gcggccagtt
     1201 ttgccgtcgt gatacgttgg cttccatcgc tggattccag catcagcgcc agagactgaa
     1261 gtatttcctc gcgacggttc cttttcgcag tttgtttttc tgccatgtta caaaataccc
     1321 ctgaaaataa gcacttgcca ggcggcaccc acgctatgac cgcaaacgaa atgtttgcgg
     1381 ctatgttatg acgttattcg gatgcgtatg tgttactgac gaccagagtg accaaagccg
     1441 ccttcaccgc ggtcggtggc gtcgaaatct tccaccagat taaattcagc ctgtactacc
     1501 ggaacaaaaa tcatctgggc gatgcgttcg ccaggttgaa tggtgaagct gtcctgacca
     1561 cggttccaca cggaaatcat caactggccc tgatagtcag aatcgatcaa tcctaccagg
     1621 ttaccaagca cgataccgtg cttatgtccc aatccggagc gcggcagcat cattgccgcc
     1681 agtgaaggat cggcaatatg aatcgccagc ccggtcggaa ccagcgtagt gtcacccgga
     1741 gccagttcta cggcgtcgtc gagacaggca cgcaggtcaa gtccggcaga gccagaggtg
     1801 gcataagtcg ggagcggaaa ttccttccca acgcgcgggt ccagaatctt aacgtcgatt
     1861 tttttcatca taacgggtca cgatctcgtc gagtaataat tggccaagga gctctttgcg
     1921 ctcaagcggt aagactttat ctccgtcctg ccagaaaagg tgtaatgcgt tgttgtcgct
     1981 gttaaatcct tgagttggct gggaaacatc gttcgcgcag atcagatcaa ggtttttacg
     2041 gatacgtttt tgccgggcgt attcttccac attatttgtt tcggcggcaa atccaacgac
     2101 gtagggtcga tggtctttta gtgcggcaac gcctgcgacg atatcggggt ttttaaccat
     2161 ttttattgtt aattcatcac cctgcgtggc ctgctttttg attttctctg gggccacggt
     2221 agctgcgcga taatccgcca cggcggcgca accgataaaa atattttgct gctgtacaga
     2281 agcattcacg gccgcttcca tttccagcgc agtcatcaca tcaacacgtt taacaaacgg
     2341 tggcgtcggt agtgaaaccg gacctgatac cagcgtgacg ttcgcgccac gacgggcagc
     2401 ggcggcggcg atagcaaaac ccatcttgcc ggagctgtga ttagagatat aacgcacggg
     2461 atcgagcggt tcacgcgtcg ggccggcggt aatcataatg ttcagatgtt tcaggtcgtt
     2521 gacgggcgaa aaatgcgcca ccgccatatc cacaatggtt aacggatcga gcattcgccc
     2581 aggaccgata tcaccacaag cctgactgcc actgtctggc ccccagatga gcaaaccacg
     2641 ggaagcaagc acctctaaat tatgctgcgt ggcagcggca cggtacatct gctggttcat
     2701 ggcggggagc acggctacag gcgcaggtgt agccagacaa atcgtcgata ccaggtcatt
     2761 cgccattccg gcagcaacac gggcaatcaa atctgccgtg gcaggggcga gaatcactaa
     2821 atcagcccat ttacccagct caatatggcc catagcggct tcggctgctg ggtccagcag
     2881 actgtcggaa acgggataac cagaaaccgc ctgcaagcta agtggggtga taaaggcttt
     2941 tgccgcttcg gtcatggcta cgcggacgtc ggccccgcgc tcgcgcaaac gacgcaccag
     3001 ttcaggggtt ttataggcag caataccgcc gctgacgccg agaacgattt ttttaccggc
     3061 caggctcatg atgatttttc ctgttgggtg acaccagaag ttggcgattt tatcacaata
     3121 cttttgttgt cgtgccttca cctgagaatc actttgcgag gcgttttcca ggattgaaaa
     3181 ctggccgtcg atttaacgga acggctatga caggatgcga gcaccacaaa ggaggtgaag
     3241 gtgaaaaaca atgcacagct gttgatgccg cgcgaaaaaa tgctgaagtt tggtattagc
     3301 gccttaacgg atgtcgagct gctggcgcta tttctgcgta ccggaacgcg cggtaaagat
     3361 gtattaaccc tggcaaaaga gatgctggag aatttcggct ctctttatgg cttgttaacc
     3421 tctgaatatg aacaatttag tggtgttcat ggaattggcg tggcgaaatt tgcccagtta
     3481 aaggggattg ctgaactagc gcggcgttac tacaacgtgc ggatgcgtga agaaagccct
     3541 ttactcagcc cggagatgac gcgggaattt ttacaaagcc agctcacggg ggaggagcgg
     3601 gagatcttta tggtgatctt tctcgactca caacaccggg ttataacgca tagccgtctt
     3661 ttttccggca cgctaaacca tgttgaagtc catcctcggg aaattatccg cgaagcgata
     3721 aaaataaatg cctcggcgct gatccttgca cataatcacc cttcgggttg tgctgaaccc
     3781 agtaaagcgg ataaactcat tactgaacgg ataataaaga gttgtcagtt catggattta
     3841 cgcgtgctcg accatatcgt gattgggcgt ggagagtatg tttcttttgc cgaacgcggc
     3901 tggatttaac ccgctatgcg cgatccttcg ggatctttgt ctgttcggga cttgagcaca
     3961 tcgctgagtc agcgtatact acgccacctt tgagaatctc gggtttggca tttgggcctg
     4021 gcaatcgaga gttcacatag aactgcgatg accgggctgt aaagcctgac gaggcgccaa
     4081 taccccatac gaagctcgag ctaatttgat ttttggagaa tagacatgtc ccgagtctgc
     4141 caagttactg gcaagcgtcc ggtgaccggt aacaaccgtt cccacgcact gaacgcgact
     4201 aaacgccgtt tcctgccgaa cctgcactct caccgtttct gggttgagag cgagaagcgt
     4261 tttgtcaccc tgcgcgtatc tgctaaaggt atgcgtgtaa tcgataaaaa aggcatcgat
     4321 acagttctgg ctgaactgcg tgcccgtggc gaaaagtact aagtacttag aggaaataaa
     4381 tcatggctaa aggtattcgt gagaaaatca agctggtttc ttctgctggt actggtcact
     4441 tctataccac tacgaagaac aaacgtacta agccggaaaa actggaactg aaaaaattcg
     4501 atccagttgt tcgccagcac gtgatctaca aagaagcgaa aatcaaataa tttccgcttt
     4561 gatgtaacaa aaaacctcgc tccggcgggg ttttttgtta tctgcttgcc cccatattga
     4621 ctgcatctgt tcattcctgg agatgctatg cctgaattac ccgaagttga aaccagccgt
     4681 cgcggcatag aaccgcatct cgttggtgca accattcttc atgcggtggt gcgcaacgga
     4741 cgcttgcgct ggccggtttc agaagagatc taccgtttaa gcgaccaacc agtgcttagc
     4801 gtgcagcggc gggctaaata tctgctgctg gagctgcctg agggctggat tatcattcat
     4861 ttggggatgt ctggcagcct gcgcatcctt ccagaagaac ttccccctga aaagcatgac
     4921 catgttgatt tggtgatgag caacggtaaa gtgctgcgct acaccgatcc gcgccgcttt
     4981 ggtgcctggc tgtggaccaa agagctggaa gggcacaacg tgctggccca tcttggaccg
     5041 gagccgctta gcgacgattt caacggtgag tacctgcatc agaagtgcga gaagaaaaaa
     5101 acggcgatta aaccgtggct gatggataac aagctggtgg taggggtagg gaatatctat
     5161 gccagcgaat ctctgtttgc ggcggggatc catccggatc ggctggcgtc atcactgtcg
     5221 ctggcagagt gtgaattgct ggctcgggtg attaaagcgg tgttgctgcg ttcgattgag
     5281 cagggcggta caacgctgaa agattttctg caaagcgacg gtaaaccggg ctatttcgct
     5341 caagaattgc aggtttacgg gcgaaaaggc gagccgtgtc gtgtgtgcgg tacgccgatt
     5401 gtggcgacta aacatgcgca gcgggcaacg ttttattgtc ggcagtgcca gaagtaattg
     5461 atgcgcgccg gatggcatac catccggcat aaacgctacg ctaacttcgc catcagcgcc
     5521 tgatggacat tctccggcag gaaatgggtg acatcgccct gatggcgcgc cacctctttc
     5581 accaacgatg aagagataaa cgaccactct ttcgacggca tcagaaacac actttccagt
     5641 tccggcatta agtggcgatt catatgcgcc agctgcattt catattcaaa atccgccacc
     5701 gcacgcaggc cacgaatcag caccgtagcg tgttgattac gggcgaagtt cgccatcaaa
     5761 tcactaaacc cgaccacttc cacgttcccc agatgcgcgg ttgcctgctg tgccagcgcc
     5821 acacgctctt ccagagtaaa catcggtttt ttactggggc tggcggcaat cgccagaata
     5881 acgtgatcga acatttgcgt ggcgcgcgtc acgatatcga tatgaccatt ggtaatggga
     5941 tcgaaagtac ccggataaat cgcccgtttt tgcataacaa cctcaatgcg ttttcggtgg
     6001 caggtaaggt tccagcagtt gaagcaggcg ctgtagcgcg ccctggtttt gatacagtac
     6061 ttcaacggca tgacggccat agaaactacg gtaatcggca tcggtgagta aagaggaaac
     6121 ctcttttgca agcgtagtgg catcggtaac ggtaatcagc ccgcttgcct gttccagccg
     6181 cgcgcaaatg tctttaaagt taaaagtatg cggccccatc aataccggaa tagcgtgtgc
     6241 ggcagcttcc agcggattat gcccaccacg ttcaaccagt gaaccgccaa caaaggcgag
     6301 atcggcaatg ccgtacagta acatcagctc gcccatcgta tcgccaacca caacctgcgt
     6361 gctggtggaa gggacttccc ctgaagagcg tgtgatatag cttagtccag cctggcggac
     6421 aaggttaatc gcatccggga agcgttccgg atgacggggt accaggatga gcaataaatt
     6481 agggaattgc tgtaacaatg cctgatgtgc ggcgatcacc acactctctt cgccttcgtg
     6541 agtgctggtg gcaatccata ccgggcggtg tggtgcccac tggcggcgca gcgtcacggc
     6601 tttagcagcc agttgcggcg ttacagaaat atcaaatttc aggctaccgg taacggtcac
     6661 ctgattattt tttgcgccca gcgccacaaa acgcgcgcca tcttcttcat tttgcgcagc
     6721 aatcagtgta atacgacgca gcaagcggcg gacgaattta cccagtttgg cgtaacctgc
     6781 ggccgagcgg gcagagagtc gcgcgttagc gatcaccagc ggaattttac gtttatgtag
     6841 cgccgcaatc aggttaggcc atagttcggt ttccataatc aacaccagtt tagggtcgac
     6901 tttattcagg aaacgattaa gtgcatcggg caggtcatac ggcagataaa cgtgctgaac
     6961 atccttcccg aaagccgatt gtacgcgctc cgaaccggtt ggcgtcatgg ttgtaacggt
     7021 aatcggtaaa tcaggataac gatgacgcag cgcgcgcacc aacgggattg ccgccagagt
     7081 ttcaccgacg gagacggagt gcagcataat gccgcctggt tttagcggat ggcggtaaaa
     7141 accgtaacgt tcaccccagc gttttcgata ggccggagcc ttacgtccgc gcacccagag
     7201 ccgtatccag atcagcggct gaataaggta gagaagggcg gtgtaaagca attcgagcat
     7261 agtaaatagc tgacttatgg atgtgctggg gattctatgt atttagctgt ggctttacca
     7321 ttacttttcc cgtttttgac ttaaatagca atttacccgc caattcttgc tgaacatcta
     7381 tacttttttt gtatttttat gcgattcatt gatttctgca gtacgtgcag gaaggaatcg
     7441 cagagcaaca gtgaaagaat atggttgcta atgattttat ttgattgatt tctttattga
     7501 aaatcaacaa tgtgcttaaa aatcattcgg gtcggattgc cgcgaaagaa gtgatacact
     7561 agcgcgtcaa tatcagtgcg gtatcagtat tcaggtagct gttgagcctg gggcggtagc
     7621 gtgctttttc tgcttcacct taatcggata atctcaacaa aaagagttac ttgtggataa
     7681 gccatttcga agaattttgc tcattaagat gcgttttcat ggggatatgt tattaactac
     7741 tcccgtcatt agttcgctga aaaaaaatta ccctgacgca aaaatcgatg tgctgcttta
     7801 tcaggacacc atcccgatcc tgtctgaaaa tccagagatt aacgcgctct acggcataaa
     7861 aaataaaaaa gcaaaagcct cagaaaaaat cgccaacttt tttcatctca tcaaggtatt
     7921 acgtgccaat aagtatgacc ttatcgtcaa tcttaccgat caatggatgg ttgctatact
     7981 ggttcgctta ttaaatgccc gtgtgaaaat ttcccaggat tatcatcatc ggcagtctgc
     8041 tttttggcgt aaaagtttca cccatttggt gccgttgcag ggtggaaatg tggtggaaag
     8101 taacttatcc gtgctgacac cattgggact tgattcgttg gtgaagcaga caaccatgag
     8161 ttacccgcct gcaagctgga aacgtatgcg tcgcgaactt gatcacgctg gtgttggaca
     8221 aaattatgtg gttatccaac ctacggcgcg gcaaatcttc aaatgctggg acaacgccaa
     8281 gttttccgct gtgattgatg ccttacatgc tcgtggttat gaagtcgttc tgacgtccgg
     8341 cccagataaa gacgatctgg tctgcgtcaa tgaaattgcg cagggatgcc agacgccacc
     8401 agtaacggcg ctggccggaa aggtgacctt cccggaactt ggtgcgttaa tcgatcatgc
     8461 gcagctgttt attggcgttg attccgcacc ggcgcatatt gccgcagcag ttaatacgcc
     8521 gctgatatcg ctgtttggtg cgacagacca tattttctgg cgtccctggt caaataacat
     8581 gattcaattc tgggcgggag attaccggga aatgccaacg cgcgatcagc gtgaccgaaa
     8641 tgagatgtat ctttcggtta ttccggcggc agatgtcatt gctgctgtcg ataaattact
     8701 gccctcctcc acgacaggta cgtcgttatg atcgttgctt tttgtttata taaatatttt
     8761 ccctttggcg gtttgcagcg cgattttatg cgtattgctc agacagtcgc cgcccgaggt
     8821 catcatgttc gggtttatac ccagtcgtgg gaaggcgaat gtcctgatgt atttgaactg
     8881 atcaaagtgc cggttaaatc gcataccaat cacgggcgca atgcggagta ttttgcctgg
     8941 gtgcaaaaac atttacgcga acatcccgtc gataaagtcg ttggattcaa caaaatgccg
     9001 gggctggacg tttattatgc cgctgatgtt tgttatgccg agaaagtagc gcaggaaaaa
     9061 ggctttttct atcgcctgac gtcacgttat cgccattatg ccgcctttga gcgggcaacc
     9121 ttcgaacagg gcaagccgac acagctgctg atgctgacag ataagcaaat cgccgatttc
     9181 cagaaacatt atcagactga agtggagcgt tttcatattc tgccaccggg gatttatcct
     9241 gatcgtaaat atagccagca gccagccaat agccgtgaaa tcttccgtaa gaagaatgga
     9301 ataaccgaac aacaatattt attgttgcag gttggttcag acttcacgcg taaaggtgtc
     9361 gatcgttcca ttgaagcact tgcttcgtta ccggattcgc tgcgccacaa cacattgcta
     9421 tatgttgttg ggcaggataa accgcgaaaa tttgaggcgc tggcagaaaa acgcggcgtg
     9481 cgcagtaatg ttcacttctt ctcggggcgc aacgatgtct cggaattaat ggcggcggcg
     9541 gatttattac tgcatcctgc ctaccaggaa gccgcgggaa ttgtactgct ggaagcgata
     9601 actgcaggat taccggtact aacaacagcc gtttgtggct atgcgcatta tattgtcgac
     9661 gccaattgcg gcgaggctat tgctgagcca ttccgccagg aaacattgaa tgagatttta
     9721 cgcaaagcgt taacgcaatc ttcattgcgc caggcctggg cggaaaatgc gcgacattat
     9781 gctgatacac aagatttata cagtctgcca gagaaagcgg cggacatcat aacgggtggt
     9841 ctggatggtt gaacttaaag agccgtttgc cacgttatgg cgcggtaaag atccttttga
     9901 ggaagttaaa accttgcagg gtgaggtatt tcgtgaactg gaaactcgcc gcactctgcg
     9961 ctttgaaatg gcgggcaaaa gctattttct caaatggcat cgcggcacga ccctgaaaga
    10021 gataatcaaa aatttactct cattgcggat gccagtatta ggcgcagacc gcgaatggaa
    10081 tgcgattcat cgactgcggg atgtcggcgt tgatactatg tatggggtgg cattcggcga
    10141 aaaaggcatt aatccgctca ccagaacctc gtttattata accgaagatc tgacaccaac
    10201 catcagtctg gaagattact gtgctgactg ggcgactaac ccaccagatg ttcgcgtaaa
    10261 gcgtatgctt attaagcgtg tcgcgacgat ggtgcgcgat atgcatgctg cgggcattaa
    10321 ccaccgtgac tgttatatct gtcatttcct gctgcacttg cctttttccg gtaaggaaga
    10381 ggagttaaaa atttcagtaa ttgacctgca ccgggcgcag cttcgcacgc gcgttccacg
    10441 tcgttggcgc gataaagatc ttattggact ttatttttct tcgatgaata ttggcctgac
    10501 tcagcgggat atctggcggt ttatgaaagt gtattttgtc gccccgctta aagacattat
    10561 caagcaggaa caagggctgc tgtcgcaagc agaagcaaaa gccacaaaaa tcagggaaag
    10621 aacgattaga aaatcgttgt aatttccgga gagaaatatg agtgcccact attttaatcc
    10681 acaagagatg atcaataaga caatcatctt cgatgaaagg ccagcggcgt cagtggcatc
    10741 atcattccat gtttcttatg gcattgataa aaactttctt tttggttgtg gtgtttcaat
    10801 cacgtcagtt ttgttacata acaacgacgt gagttttgtt ttccacgttt ttattgatgc
    10861 tatccctgaa gccgatatcc agcgtttagc ccaattggcg aaaagctatc gtacctgtat
    10921 ccagatccat ctagtaaatt gtgaacggct taaggcatta ccgacgacca aaaattggtc
    10981 tattgccatg tatttccgtt ttgtaattgc agattacttt attgatcaac aagataagat
    11041 cctttacctg gatgctgata tcgcctgtca gggaaactta aagccgctga taacaatgga
    11101 tcttgccaat aacgttgctg ctgttgttac tgaacgcgat gctaactggt ggtcgttacg
    11161 gggtcaaagt ctgcagtgta atgaacttga aaagggttac tttaattcag gtgtcctgtt
    11221 aattaataca ctagcgtggg cgcaggagtc cgtttctgct aaagcgatgt cgatgcttgc
    11281 tgataaagcc atcgtttccc gtttaaccta tatggatcaa gatatcctta atcttatcct
    11341 gttagggaaa gttaaattca ttgatgctaa atacaatacg caatttagtt taaattatga
    11401 attaaaaaaa tcatttgttt gtccaattaa tgatgaaacc gtattaattc attatgtcgg
    11461 cccgacaaaa ccctggcatt actgggccgg ttatccaagt gcgcaacctt ttatcaaagc
    11521 caaagaagca tcgccctgga aaaatgaacc gttaatgcgg ccagttaact caaactatgc
    11581 tcgttattgc gccaagcata attttaaaca aaataaacca attaacggga taatgaatta
    11641 tatttattat ttttatttaa agataataaa atgaaattga gaaaagttaa atgaatgaat
    11701 ttataaaaga acggtt
//
LOCUS       DAOTRK010000143        11645 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.144, whole genome shotgun sequence.
ACCESSION   DAOTRK010000143 DAOTRK010000000
VERSION     DAOTRK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.144"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            79..624
                     /locus_tag="QIE41_003811"
     CDS             79..624
                     /locus_tag="QIE41_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDS3532647.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     MAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            599..2524
                     /locus_tag="QIE41_003812"
     CDS             599..2524
                     /locus_tag="QIE41_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDS3532648.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2521..2727
                     /locus_tag="QIE41_003813"
     CDS             2521..2727
                     /locus_tag="QIE41_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDS3532649.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2724..4325
                     /locus_tag="QIE41_003814"
     CDS             2724..4325
                     /locus_tag="QIE41_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDS3532650.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYASYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVTMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAHFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4306..5625
                     /locus_tag="QIE41_003815"
     CDS             4306..5625
                     /locus_tag="QIE41_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDS3532651.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5635..5967
                     /locus_tag="QIE41_003816"
     CDS             5635..5967
                     /locus_tag="QIE41_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDS3532652.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGVLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6023..7048
                     /locus_tag="QIE41_003817"
     CDS             6023..7048
                     /locus_tag="QIE41_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDS3532653.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7090..7488
                     /locus_tag="QIE41_003818"
     CDS             7090..7488
                     /locus_tag="QIE41_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDS3532654.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7500..7853
                     /locus_tag="QIE41_003819"
     CDS             7500..7853
                     /locus_tag="QIE41_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDS3532655.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLIIGEENFWVDRVTPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7865..8443
                     /locus_tag="QIE41_003820"
     CDS             7865..8443
                     /locus_tag="QIE41_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3532656.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8440..8835
                     /locus_tag="QIE41_003821"
     CDS             8440..8835
                     /locus_tag="QIE41_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3532657.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8843..9583
                     /locus_tag="QIE41_003822"
     CDS             8843..9583
                     /locus_tag="QIE41_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3532658.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASASVVKGQSTTLTVAFQPEGVTDKSFRAVSSDKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            9599..10012
                     /locus_tag="QIE41_003823"
     CDS             9599..10012
                     /locus_tag="QIE41_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDS3532659.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLALMKQQAESDSNRKF
                     TVEDVIRTGAFVVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAENVVY
                     RLSGMYEFVVNNAPEQADDAGPAEPVSAGKCSTVS"
     gene            9994..10428
                     /locus_tag="QIE41_003824"
     CDS             9994..10428
                     /locus_tag="QIE41_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDS3532660.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREAGEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPYVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10421..>11645
                     /locus_tag="QIE41_003825"
     CDS             10421..>11645
                     /locus_tag="QIE41_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDS3532661.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSINRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGITSLAVATGALAYAWYQGDSTLSNFNKTLVLSGNQA
                     GLTADRMLALSRSGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDGAGALQAANEAA
                     TKGFDDQTRKLKDNMGTLETWADKTAQAFKSMWDAVLDIGRPDSSADMLAKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEKRENARLGLSAATLQADLEKASEMAAKD
                     RAESEASRLKYTEEAQ"
BASE COUNT         2655 a         2968 c         3613 g         2409 t
ORIGIN      
        1 gtccgacatg aaaacgccta aaaaagtcat tttcgggcac tttcatgtcg gcccctgtat
       61 ttattgtgag actgtttcat gaaggttaat aaaaagaaac ttgccgaaat tttcaacgtg
      121 gatccgcgaa cgattgaacg ctggcagtct cagggactcc cttgcgtctc cggaggtggt
      181 aagggcgttg aatctgtatt tgataccgcc atggcaattc agtggtatgc gcagagggaa
      241 gctgatatcg aaaatgaaaa actccgtaaa gaggttgagg attacagggc tgccagtgag
      301 gcagatctcc agcctgggac tattgagtac gaacgccatc gacttacgcg tgcgcaggcc
      361 gacgcacagg agctgaagaa tgccagagac tccgcagagg tggtggaaac cgcattctgt
      421 actttcgtgc tgtcacggat cgcaggtgaa attgccagta ttcttgacgg gatccctctc
      481 tcggtacagc ggcgttttcc ggaactggaa aaccgacatg ttgatttcct gaaacgggat
      541 atcatcaaag ccatgaacaa agcagccgcg ctggatgaac tgataccggg gttgctgagt
      601 gaatatatcg aacagtcaga ttgatattct gcggcgtgat gtacgcgccg ggctgcgagc
      661 cctgttcagg ccggagccac agactgccgt tgaatgggcg gatgccagtt actatctccc
      721 gaaagaatcc gcataccagg aagggcgctg ggaaacactg ccctttcagc gggccatcat
      781 gaatgcgatg ggcagcgact acatccgtga ggtgaatgtg gtgaagtctg cccgtgtcgg
      841 ttattccaaa atgctgctgg gtgtttatgc ctacttcata gagcataagc agcgcaacac
      901 ccttatctgg ttgccgacgg atggtgatgc cgagaacttt atgaaaaccc acgttgagcc
      961 gactattcgt gatattccgt cgctgctggc gctggccccg tggtatggca aaaagcaccg
     1021 ggataacacg ctcaccatga agcgtttcac caatgggcgt ggtttctggt gcctgggcgg
     1081 taaagcggca aaaaactacc gtgaaaagtc agtggatgtg gcgggttatg atgaacttgc
     1141 tgcctttgat gatgatattg aacaggaagg ctctccgacg ttcctgggtg acaagcgtat
     1201 tgaaggctcg gtctggccaa agtccatccg tggctccacg cccaaagtga gaggcacctg
     1261 tcagattgag cgtgcagcca gtgaatcccc gcattttatg cgttttcatg ttgcctgccc
     1321 gcactgtggg gaggagcagt accttaaatt tggcgataaa gagacgccgt ttggcctcaa
     1381 atggacgccg gatgatccct ccagcgtgtt ttatctctgc gagcataatg cctgcgtcat
     1441 ccgtcagcag gagctggact ttactgatgc ccgttatatc tgcgaaaaga ccgggatctg
     1501 gacccgtgat ggcattctct ggttttcgtc atccggtgaa gagattgagc caccggacag
     1561 tgtgaccttt cacatctgga cggcgtacag cccgttcacc acctgggttc agattgtcaa
     1621 agactggatg aagacgaaag gggatacggg aaaacgtaaa accttcgtga acaccacgct
     1681 cggtgagaca tgggaagcga aaatcggcga acgtccggat gctgaagtga tggcagagcg
     1741 gaaagagcat tattcagcgt ccgttcctga ccgtgtggct tacctgaccg ccggtatcga
     1801 ctcccagctg gatcgctacg aaatgcgcgt atggggatgg gggccgggtg aggaaagctg
     1861 gctgattgac cggcagatta ttatgggccg ccacgacgat gaacagacgc tgctgcgtgt
     1921 ggatgaggcc atcaataaaa cctatacccg ccggaatggt gcagagatgt cggtatcccg
     1981 tatctgctgg gatactggcg ggattgaccc gaccattgtg tatgaacgct cgaaaaaaca
     2041 tgggctgttc cgggtgatcc ctattaaagg ggcatccgtc tacggaaagc cggtggccag
     2101 catgccacgt aagcgaaaca aaaacggggt ttaccttacc gaaatcggta cggataccgc
     2161 gaaagagcag atttataacc gcttcacact gacgccggaa ggggatgaac cgcttcccgg
     2221 tgccgttcac ttcccgaata acccggatat ttttgatctg accgaagcgc agcagctgac
     2281 ggctgaagag caggtcgaaa aatgggtgga tggcaggaaa aaaatactgt gggacagcaa
     2341 aaagcgacgc aatgaggcac tcgactgctt cgtttatgcg ctggcggcgc tgcgcatcag
     2401 tatttcccgc tggcagctgg atctcagtgc gctgctggcg agcctgcagg aagaggatgg
     2461 tgcagcaacc aacaagaaaa cactggcaga ttacgcccgt gccttatccg gagaggatga
     2521 atgacgcgac aggaagaact tgccgctgcc cgtgcggcac tgcatgacct gatgacaggt
     2581 aaacgggtgg caacagtaca gaaagacgga cgaagggtgg agtttacggc cacttccgtg
     2641 tctgacctga aaaaatacat tgctgagctg gaagtgcaga ccggcatgac acagcgacgc
     2701 aggggacctg caggatttta tgtatgaaaa cgtccaccat tcccaccctt ctggggccgg
     2761 acggcatgac atcactgcgt gaatatgcca gttatcacgg cggtggcagc ggatttggtg
     2821 ggcagttgcg ggcgtggaat ccaccgagtg aaagtgtgga tgcagccctg ctgcccaact
     2881 ttacccgtgg caatgcccgc gcggacgatc tggtacgcaa taacggctat gctgccaacg
     2941 ccatccagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa
     3001 gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag
     3061 cggcatggaa agagtttgcc gaggacgact gttgctgcat tgacgttgag cgaaaacgca
     3121 cgttcaccat gatgattcgg gaaggtgtga ccatgcatgc ctttaacggt gaactgttcg
     3181 ttcaggccac ctgggatacc agcccgtcgc gactgttccg gacacagttc cggatggtca
     3241 gtccgaagcg tatcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg
     3301 tgcagattaa tgacagcggc gcggcgctgg gatattacgt cagcgaggac ggctatcctg
     3361 gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcctcgt
     3421 tcattcacgt ttttgaaccc gtggaggacg gacagacccg cggtgcaaat gtgttttaca
     3481 gcgtgatgga gcagatgaag atgctcgaca cgttacagaa cacgcagctg cagagcgcca
     3541 ttgtgaaggc gatgtatgcc gccaccattg agagtgagct ggatacgcag tcagcgatgg
     3601 attttattct gggtgcgaac agtcaggagc agcgggaaag gctgacgggc tggattggtg
     3661 aaattgccgc gtattacgcc gcagcaccgg tccgtctggg aggcgcaaaa gtgccgcacc
     3721 tgatgccggg tgactcactg aacctgcaga cggctcagga cacggataac ggctactccg
     3781 tgtttgagca gtcactgctg cggtatatcg ctgccggact gggtgtctcg tatgagcagc
     3841 tttcccggaa ttacgcccag atgagctact ccacggcacg ggccagcgcg aacgagtcgt
     3901 gggcgcactt tatggggcgg cgaaaattcg tcgcatcccg tcaggcgagc cagatgtttc
     3961 tgtgctggct ggaagaggcc atcgttcgcc gcgtggtgac gttaccttca aaagcgcgct
     4021 tcagttttca ggaagcccgc agtgcctggg ggaactgcga ctggataggc tccggtcgta
     4081 tggccatcga tggtctgaaa gaagttcagg aagcggtgat gctgatagaa gccggactga
     4141 gcacctacga gaaagagtgc gcaaaacgtg gtgacgacta tcaggaaatt tttgcccagc
     4201 aggtccgtga aacgatggag cgccgtgcag ccggtcttaa accgcccgcc tgggcggctg
     4261 cggcatttga atccggactg cgacaatcaa cagaggagga gaagagtgac agcagagctg
     4321 cgtaatctcc cgcatattgc cagcatggct tttaatgagc cgctgatgct tgaacccgcc
     4381 tatgcgcggg ttttcttttg tgcgcttgca ggccagcttg ggatcagccg cctgacggat
     4441 gcagtatccg gcgacagcct gactgccgga gaggcacccg cggcactggc gttatccggt
     4501 gatgatgacg gaccacgaca ggcccgcagc tatcaggtca tgaacggcat cgccgtgctg
     4561 ccggtgtccg gtacgctggt cagccggacg cgggcgctgc agccgtactc ggggatgacc
     4621 ggttacaacg gcattatcgc ccgtctgcaa caggctgcca gcgatccgat ggtggacggc
     4681 attctgctgg atatggacac accgggcggg atggtggcgg gagcatttga ctgtgctgac
     4741 atcatcgccc gtgtgcgaga cataaaaccg gtatgggcgc tggccaacga catgaactgc
     4801 agtgcaggtc agctgcttgc cagcgccgcc tcccggcgtc tggtcacgca gaccgcccgg
     4861 acaggctcca tcggcgtcat gatggctcac agcaattacg gtgctgcgct ggagaaacag
     4921 ggcgtggaaa tcacgctgat ttacagcggc agccataagg tggatggcaa tccctacagc
     4981 catcttccgg atgacgtccg ggagacactg cagtcccgga tggacgcaac ccgccagatg
     5041 tttgcgcaga aggtgtcggc atataccggc ctgtctgtgc aggctgtgct ggataccgag
     5101 gctgcagtgt acagcggtca ggaggccatt gatgccggac tggctgatga acttgttaac
     5161 agtaccgatg cgatcaccgt tatgcgtgat gcactggatg cacgtaaatc ccgtctctca
     5221 ggagggcgaa tgaccaaaga gactcaatca acaactgttt cagccactgc ttcgcaggct
     5281 gacgttactg acgtggtgcc agcgacggag ggcgaaaacg ccagcgcggc gcagccggac
     5341 gtgaacgcgc agatcaccgc tgcggttgcg gcagaaaaca gccgcattat ggggatcctc
     5401 aactgtgagg aggctcacgg acgcgaagaa caggcccgcg tgctggcaga aacccccggt
     5461 atgaccgtgg aaacggcccg ccgcattctg gcagctgcac cacagagtgc acaggcgcgc
     5521 agtgacactg cgctggatcg tctgatgcag ggggcaccgg caccgctggc tgcaggtaac
     5581 ccggcatctg atgccgttaa cgatttgctg aacacaccag tgtaagggat gtttatgacg
     5641 agcaaagaaa cctttaccca ttaccagccg cagggcaaca gtgacccggc tcataccgca
     5701 accgcgcccg gcgtattgag tgcgaaagcg cctgcaatga ccccgctgat gctggacacc
     5761 tccagccgta agctggttgc gtgggatggc accaccgacg gtgctgccgt tggcattctt
     5821 gcggttgctg ctgaccagac cagcaccacg ctgacgttct acaagtccgg cacgttccgt
     5881 tatgaggatg tgctctggcc ggaggctgcc agcgacgaga cgaaaaaacg gaccgcgttt
     5941 gccggaacgg caatcagcat cgtttaactt tacccttcat cactaaaggc cgcctgtgcg
     6001 gcttttttta cgggattttt ttatgtcgat gtacacaacc gcccagctgc tggcggcaaa
     6061 tgagcagaaa tttaagtttg atccgctgtt tctgcgtctc tttttccgtg agagctatcc
     6121 cttcaccacg gagaaagtct atctctcaca aattccggga ctggtaaaca tggcgctgta
     6181 cgtttcgccg attgtttccg gtgaggttat ccgttcccgt ggcggctcca cctctgaatt
     6241 tacgccggga tatgtcaagc cgaagcatga agtgaatccg cagatgaccc tgcgtcgcct
     6301 gccggatgaa gatccgcaga atctggcgga cccggcttac cgccgccgtc gcatcatcat
     6361 gcagaacatg cgtgacgaag agctggccat tgctcaggtc gaagagatgc aggcagtttc
     6421 tgccgtgctt aagggcaaat acaccatgac cggtgaagcc ttcgatccgg ttgaggtgga
     6481 tatgggccgc agtgaggaga ataacatcac gcagtccggc ggcacggagt ggagcaagcg
     6541 tgacaagtcc acgtatgacc cgaccgacga tatcgaagcc tacgcgctga acgccagcgg
     6601 tgtggtgaat atcatcgtgt tcgatccgaa aggctgggcg ctgttccgtt ccttcaaagc
     6661 cgtcaaggag aagctggata cccgtcgtgg ctctcattcc gagctggaga cagcggtgaa
     6721 agacctgggc aaagcggtgt cctataaggg gatgtatggc gatgtggcca tcgtcgtgta
     6781 ttccggacag tacgtggaaa acggcgtcaa aaagaacttc ctgccggaca acacgatggt
     6841 gctggggaac actcaggcac gcggtctgcg cacctatggc tgcattcagg atgcggacgc
     6901 acagcgcgaa ggcattaacg cctctgcccg ttacccgaaa aactgggtga ccaccggcga
     6961 tccggcgcgt gagttcacca tgattcagtc agcaccgctg atgctgctgg ctgatcctga
     7021 tgagttcgtg tctgtacaac tggcgtaatc atggcccttc ggggccattt tctctctgtg
     7081 gaggagttca tgacgaaaga tgaactgatt gcccgtcttc aggagctggg tgagcaactg
     7141 aaccgcgatg tcagtttgac gggaacgaaa gaagaactgg cgcttcgtgt ggcagagctg
     7201 gaagaagagc ttgatgacac ggatgaaact gccggtcagg acacccctct cagccgggaa
     7261 aatgtgctga ccgggcatga aaatgaggtg gtatcagcgc agccggatac cgtgattcag
     7321 gatacggctg aactggtcac ggtcgtggca ctggtgacgc tgcatactga tgcgcttcac
     7381 gccacgcggg ataaacctgt ggcatttgtg ctgccgggaa cggcgtttcg tgtctctgcc
     7441 ggtgtggcag ccgaaatgac agaacgtggc ctggccagaa tgcaataacg ggaggcgctg
     7501 tggctgattt cgataacctg ttcgatgctg ccattgcccg cgctgatgaa acgatacgcg
     7561 ggtacatggg aacgtcagcc accattacat ccggtgagca gtccggtgcg gtgatacgtg
     7621 gtgtttttga tgaccctgaa aatatcagct atgccggaca gggcgtgcgc gttgaaggct
     7681 ccagcccgtc cctgtttgtc cggactgatg atgtgcggca gctgcggcgt ggagacacgc
     7741 tgatcatcgg tgaggaaaac ttctgggtag atcgggttac gccggatgat ggcggaagct
     7801 gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa ccgtcgccgc tgaaaggggg
     7861 atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac ctcagccgta tcagcaaaac
     7921 ggcggtgcct ggtgccgccg caatggccat taaccgcgtt gcttcatccg cgatatcgca
     7981 gtctgcgtca caggttgccc gtgagacaaa ggtacgccgg aaactggtaa aggaaagggc
     8041 caggctgaaa agggccacgg tcaaaaatcc gcaggccaga atcagggtta accgggggga
     8101 tttgcccgta atcaggctgg gtaatgcgcg ggttgtcctg tcccgccgca ggcgtcgtaa
     8161 aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg cttgtggtgg gaaaccgtcg
     8221 tattcccggc gcgtttattc agcaactgaa aaatggccgg tggcatgtca tgcagcgtgt
     8281 ggccgggaaa aaccgttacc ccattgatgt ggtgaaaatc ccgatggcgg tgccgctgac
     8341 cacggcgttt aaacagaata ttgagcggat acggcgtgag cgtcttccga aagagctggg
     8401 ctatgcgctg cagcatcaac tgagaatggt aataaagcga tgaaacatac tgaactccgt
     8461 gcagccgtac tggatgcact ggagaagcat gacaccgggg cgacgctttt tgatggtcgc
     8521 cccgctgttt ttgatgaggc ggattttccg gcagttgccg tttatctcac cggcgctgaa
     8581 tacacgggcg aagagctgga cagcgatacc tggcaggcgg agctgcatat cgaagttttc
     8641 ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga tggagtcccg gatttatccg
     8701 gtgatgagcg atatcccggc actgtcagat ttgatcacca gtatggtggc cagtggctat
     8761 gactaccggc gcgacgatga tgcgggcctg tggagttcag ccgatctgac ttatgtcatt
     8821 acctatgaaa tgtgaggacg atatgccaac accaaatcct ctggcaccgg tgaaaggggc
     8881 cgggaccaca ctgtgggttt ataaggggaa cggtgaccct tatgcgaacc cgctttcaga
     8941 cgttgactgg tcgcgtctgg caaaagttaa agacctgaca cccggcgaac tgaccgctga
     9001 gtcctatgac gacagctatc tcgatgatga agatgcggac tggactgcga ccgggcaggg
     9061 gcagaaatct gccggagata ccagcttcac gctggcgtgg atgcccggag agcaggggca
     9121 gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt gcctataaaa tccgctttcc
     9181 taacggcacg gtcgatgtgt tccgtggctg ggtcagcagt atcggtaaag cggtgacggc
     9241 gaaggaagtg atcacccgca cggtgaaggt caccaatgtg ggacgcccgt cgatggctga
     9301 agatcgcagt acggtgacgg cggcaaccgg catgacggta acgccagcca gtgcgtccgt
     9361 agtgaaagga cagagcacca cgctgaccgt ggcattccag ccggaaggcg taaccgacaa
     9421 gagcttccgt gcggtgtctt cggataaaac aaaagccacc gtgtcggtca gtggtatgac
     9481 catcaccgtg aacggcgttg ctgcaggcaa ggtcaacatt ccggtcgtat ccggtaatgg
     9541 tgagtttgct gcggttgcag aaatcaccgt caccgccagt taatccggag agtcagcgat
     9601 gttcctgaaa accgaatcat ttgaatataa cggcgtgacc gtcacgcttt ctgaactgtc
     9661 agccctgcag cgtattgagc atctcgctct gatgaaacag caggcagaat ccgacagcaa
     9721 ccggaagttt actgtggaag acgtcatcag aaccggcgct tttgtggtgg cgatgtccct
     9781 gtggcataac catccgcaga agacgcagat gccgtccatg aatgaagccg ttaaacagat
     9841 tgagcaggaa gtgcttacca cctggcccac agaggcaatt tctcatgctg aaaacgtggt
     9901 gtaccggctg tccggaatgt atgagtttgt ggtgaataat gcccctgaac aggcagatga
     9961 cgccgggcct gcagagcctg tttctgcggg aaagtgttcg acggtgagct gagttttgcc
    10021 ctgaaactgg cgcgagagat ggggcgaccc gactggcgcg ccatgcttgc cgggatgtca
    10081 tccacggagt atgccgactg gcaccgcttt tacagtaccc attattttca tgatgttctg
    10141 ctggatatgc atttttccgg gctgacgtac accgtgctca gcctgttttt cagcgatccg
    10201 gatatgcatc cgctggattt cagtctgctg aaccggcgtg aggctggcga agagcctgaa
    10261 gatgatgtgc tgatgcagaa agcggcaggg cttgccggag gcgttcgttt tggcccggac
    10321 gggaatgaag ttatccccgc ttccccgtat gtggcggaca tgacggagga tgacgtaatg
    10381 ctgatgacag tatcagaagg gatcgcagga ggagtccggt atggctgaac cggtaggcga
    10441 tctggtcgtt gatttaagtc tggatgcggc cagatttgac gagcagatgg ccagagtcag
    10501 gcgtcatttt tccggtacgg aaagtgatgc gaaaaaaaca gcggcagtcg ttgaacagtc
    10561 aataaaccgg caggcgctgg ctgcacagaa agcggggatt tccgtcgggc agtataaagc
    10621 tgccatgcgt atgctgcctg cgcagttcac cgacgtggcc acgcagcttg caggggggca
    10681 gaatccgtgg ctcatcctgc tgcaacaggg tggtcaggtg aaggactcct tcggcgggat
    10741 gatccccatg ttcagggggc ttgccggtgc gatcaccctg ccgatggttg gtatcacttc
    10801 gctggcggtg gcgaccggtg cgctggcgta tgcctggtat cagggtgact caaccctgtc
    10861 caatttcaat aaaacgctgg tcctttccgg taatcaggcg ggactgacgg cagatcgtat
    10921 gctggccctg tccagatccg ggcaggcggc agggctgacg tttaaccaga ccagcgagtc
    10981 actgacggcg ctggtgaatg ccggtgtgcg tggtggtgag cagtttgagg caatcagcca
    11041 gagtgtggcg cgtttctcct ctgcatccgg tgtggaggtg gacaaggtcg ctgaagcctt
    11101 cgggaagctg accactgacc cgacgtcggg gctgacggcg atggcgcgcc agttccataa
    11161 cgtgacggcg gagcagattg cgtatgttgc tcagttgcag cgttccggcg atggggccgg
    11221 ggcattgcag gcggcgaacg aggccgcaac gaaagggttt gatgaccaga cccgtaaact
    11281 gaaagataac atgggtacgc tggagacctg ggcagacaag actgcacagg cattcaaatc
    11341 catgtgggat gcggtgctgg atattggccg cccggactcc tctgcagata tgctcgccaa
    11401 agctgaaaag gcttttgatg aagcggataa aaaatggcag tggtatcaga gccgaagcca
    11461 ccggcgcggt aaaacgtcag catttcttgc caatctccgg ggggcatggg agaagagaga
    11521 gaatgcgcga cttgggcttt cagccgccac gttgcaggca gatcttgaaa aggccagtga
    11581 gatggcagca aaagatcggg ccgagtctga ggcatcacgg ctgaaatata ccgaagaggc
    11641 gcaga
//
LOCUS       DAOTRK010000144        11640 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.145, whole genome shotgun sequence.
ACCESSION   DAOTRK010000144 DAOTRK010000000
VERSION     DAOTRK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.145"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(17..229)
                     /gene="cspA"
                     /locus_tag="QIE41_003826"
     CDS             complement(17..229)
                     /gene="cspA"
                     /locus_tag="QIE41_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDS3532662.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(510..800)
                     /locus_tag="QIE41_003827"
     CDS             complement(510..800)
                     /locus_tag="QIE41_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDS3532663.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1234..1944
                     /locus_tag="QIE41_003828"
     CDS             1234..1944
                     /locus_tag="QIE41_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDS3532664.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1994..2968)
                     /gene="ghrB"
                     /locus_tag="QIE41_003829"
     CDS             complement(1994..2968)
                     /gene="ghrB"
                     /locus_tag="QIE41_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDS3532665.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFGKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3072..3731)
                     /locus_tag="QIE41_003830"
     CDS             complement(3072..3731)
                     /locus_tag="QIE41_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDS3532666.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3884..6217
                     /gene="bisC"
                     /locus_tag="QIE41_003831"
     CDS             3884..6217
                     /gene="bisC"
                     /locus_tag="QIE41_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDS3532667.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVLSSRGALTTGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPEKPQGIRGQDEFVRVSWDEALELIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVNAEKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELSPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYGYQDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6186..6626)
                     /locus_tag="QIE41_003832"
     CDS             complement(6186..6626)
                     /locus_tag="QIE41_003832"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS3532668.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6623..7186)
                     /gene="tag"
                     /locus_tag="QIE41_003833"
     CDS             complement(6623..7186)
                     /gene="tag"
                     /locus_tag="QIE41_003833"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDS3532669.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7344..8042
                     /locus_tag="QIE41_003834"
     CDS             7344..8042
                     /locus_tag="QIE41_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDS3532670.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8271..9479
                     /locus_tag="QIE41_003835"
     CDS             8271..9479
                     /locus_tag="QIE41_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532671.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9803..11494
                     /gene="eptB"
                     /locus_tag="QIE41_003836"
     CDS             9803..11494
                     /gene="eptB"
                     /locus_tag="QIE41_003836"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDS3532672.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVREGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
BASE COUNT         2726 a         2854 c         3030 g         3030 t
ORIGIN      
        1 tgcttttaag cagagattac aggctggtta cgttaccagc tgccgggcct ttagcgccgc
       61 tttcgatggt gaaggacact ttctgacctt cgtccagaga tttgtaacca tcgttctgga
      121 tagcagagaa gtgtacgaac acgtctttag agccatcgtc aggagtgatg aagccgaagc
      181 ctttgtcagc gttgaaccat tttacgatac cagtcatttt accggacata gtgtattacc
      241 tttaataatt aagtgtgcct ttcggcgata tggcgtgctt tacagatttt gaagcgttaa
      301 aggaatgtgc actacgaggg gtatcaacga taactcttga agggacttgc cttactacac
      361 tgctttaatg gtctgtacgt caaaccgttg atgtgcatta agccacgcat tggcgggtga
      421 tgcaacaatt atttttcata tttatgatta atcggttgga aaacggtcct gtcatcagga
      481 ccgtaaacag caataaagtg gataaaagtc tattccatca actgcttact taatgccggg
      541 ttggcttgaa tcaaacgcat caattttagt tcggcacttg aaggcttcac gcgtctggat
      601 tcccattcct ttaccatggc gactgatacg cccaaaaccc gggcgaaatc atcgattttt
      661 aatcctgtcc cttttcgtaa ctgctcaatt tcggtacagg atgttgttct gtgcgtcagg
      721 gtaattttct gcgtttcatc tttaaaaaca atctgttcca ggctgctcaa cagctcatgc
      781 attggatctt tatattccat tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca
      841 tcgaagctca ttaagaatag tcgggaaaag cagacacagc ggggtgaagt cagggcatgc
      901 tctggctatg attcgattca gccagtcatt tagcgtgact tatagccacc taacgatctg
      961 ataaatgccg atatatctct taccactccc ggtttgtaaa gatttcgaaa atctaacgtt
     1021 ctgcgaaggc gtaaatctcg gtctacaaaa aaagcaacgg cactatcatt tattaacaat
     1081 gaaacaacaa attaaccttg tttcattttc tccatgattt taccaggaag attacgtaga
     1141 aagggtatcc tgcaagcctg tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt
     1201 tgtgcgcttt ttttgggtga aaggagtaag aaaatggcga caggaaagtc ctgctctcgc
     1261 tggtttgcgc ctcttgcggc gttattaatg gtagttagcc tgagtgggtg ttttgataaa
     1321 gaaggcgatc agcgtaaagc gtttatcgac ttcctgcaga atacagtgat gcgtagcggt
     1381 gaacgtctac caaccctgac tgccgatcag aaaaaacagt ttggtccttt tgtctctgat
     1441 tacgcgattc tgtatggtta ttctcagcag gtgaatcagg cgatggattc cggtctgcgc
     1501 ccggtcgtag acagcgttaa cgcaattcgc gtaccgcagg attacgttac gcaaagcggc
     1561 ccactgcgtg aaatgaacgg ttctctgggc gtactggcgc aacagctgca aaatgcgaag
     1621 ttgcaggccg atgctgcgca ctctgcgcta aaacagagcg atgacctgaa accggtcttt
     1681 gatcaggcat tcaccaaagt ggtaacgacg ccagctgatg cattgcaacc gttgattccg
     1741 gcggcacaaa ctttcacgca acaactggtg atggttggag actacattgc ccaacaaggt
     1801 actcaggtga gctttgtcgc aaatggcatc cagttcccga cgtcacagca ggcaagtgaa
     1861 tataacaaac tgattgcgcc attaccggca cagcatcagg cgtttaatca ggcctggact
     1921 acagcagtta ctgcaaccca ataaagagta aaagcccgag cgattatcgg gctttactcc
     1981 acgcagtcgc ggcttagtcc gcgacgtgcg gattcacaca gttcttctca acctttcctt
     2041 gtaacgcatc aatcaaatta tccacggcac aggcggccat gccataacgc gtctcatggg
     2101 tggcagatcc aatatgcggt actgcgacga catttgccat tgagagcaac ggcgaatcta
     2161 cggaaagtgg ctcttgttcg aagacatcca gcccggcggc gtgaatttcg cctttctgca
     2221 atgcagcaat cagtgcattt tcatcaacca ccgggccacg tccggcatta atgaaaatgg
     2281 cggaggattt cattttgccg aattgttctg cgccaaatag atgatacgtc tcatcagtta
     2341 acggcaggat caggcaaacg aaatctgact cttgtaacag tgtatccaaa tcgcagtagc
     2401 gggcgttgaa gcgttcttct gcttctttat ggtggcggcg cgcgttatag aggatgggca
     2461 tgttgaaacc aaagtgcgca cgttgtgcca gcgccatacc gatccgtccc atcccgacaa
     2521 tgcccagtgt tttatggtga acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc
     2581 attcgcctgc ttttacccgt tctgctacct ccacaacccg acgagcggta gacaacacca
     2641 gcgccatcag cgtatcggcg acggtttctg ttaatacggt tggcgtgtgc atcagcagaa
     2701 tttttcgggc ggtaagcgca tcgacatcaa aattgtcata gccgacagag atcgttgatg
     2761 tggcacgcag tttcggcatt ttttccagca atgcggcatc aacattctcg tttgaaccca
     2821 gtaaaccttc agcttcggca aaaattgctg cattttgttc gacggtttgt gggctgaggt
     2881 ttgccacctg gtgaacggtg aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg
     2941 ctttgtagag gataacggac ggcttcatgc ttctctccat tcactgataa ggcgatcacc
     3001 ttagcctgaa atatgatgaa ggaaaactga agaatgccag gtgatttctg catcacctgg
     3061 catgaaaggg attacagcgg gcttaaggta atttctacac ggcggttttg cgccttacct
     3121 tctgcagtgc tgttgctggc gattgggtta gccgggccaa ggccctgagt acggatgcgg
     3181 ctggcgtcca cgccctgggt gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag
     3241 agacgcatgt tcaggtcgtg accacccgtg ctgtcggtat aaccaatcac gttaaccgcc
     3301 gttttcggat actctttcag caccattgcc acgccggtca gggtgttagc gcctgccggt
     3361 ttcagggtcg cgctgctgct gtcgaaggtc acattgttcg gcatattgag gataatgtta
     3421 tccccgctgc gggttacgct aacaccagtg ccacgcattt tgtcacgcag cttcgcttcc
     3481 tgcacatcca tgtaataacc cacgccgccg cccagagctg cacccgctgc tgcgccaatc
     3541 agcgcgcctt taccgcgatc tttcttcgaa gaagagagcg caccaatacc cgcgccaacg
     3601 agagagccca gacctgcgcc gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg
     3661 ttagttgtgc agccagatac cgccagagcg ccactcacta cggcggcaat aagataaaca
     3721 cgtttcttca ttgttaatcc ttaataacct ttttattctt tgccacgggt tccgtggcgg
     3781 gagattatgc cgcgtgaaca tgaagattat tcctgggaat actcggaaat ttgtaagtaa
     3841 tatttaactg ctcaatacat ctaacctttc aggagtcttc ggtttggcca actcatcctc
     3901 acgatattcc gttctgactg ccgcccactg ggggcccatg ctggttgaaa ctgacggcga
     3961 aaccgtgctt agctcgcgtg gcgcgttaac cacaggaatg gaaaactcct tgcagagcgc
     4021 ggttcgcgac caggttcaca gcaatacgcg ggtacgattt ccaatggtgc gcaaaggctt
     4081 tctcgcgtca ccggaaaaac cgcaaggcat tcgcgggcag gatgaatttg ttcgtgtgag
     4141 ttgggatgag gcgctggagc ttattcacca acaacataaa cgcattcgtg aggcttatgg
     4201 tccggcatcg atttttgctg gttcctacgg ctggcgttca aacggcgtgc tgcataaggc
     4261 ctcgacatta ttacaacgct atatggcgct ggcaggcggt tataccgggc atctggggga
     4321 ttattcgacc ggtgcagcac aggcgatcat gccgtatgtc gtggggggca gcgaagttta
     4381 tcaacagcaa accagttggc ccctggtgct ggaacatagc gatgtcgtgg tgctgtggag
     4441 tgctaatcca ctcaatacgc tgaaaattgc gtggaatgca tccgatgagc aggggctttc
     4501 ttacttttcc gcactgcgtg acagcgggaa aaagctgatc tgcattgatc caatgcgatc
     4561 ggaaaccgtc gatttctttg gcgataaaat ggagtgggtg gcaccgcaca tgggcaccga
     4621 tgttgcgctg atgctgggga tcgcccatac gctggtggaa aatggttggc acgacgaagc
     4681 gtttctggcg cgttgcacca ccggttatgc cgtcttcgcc tcttatttgc tgggcgagag
     4741 tgacggaata gccaaaaccg ccgaatgggc ggcagggatt tgtggagtta acgcagagaa
     4801 aatccgcgag ctggcggctc ttttccacca aaacacgacc atgctgatgg ctggctgggg
     4861 aatgcagcgt caacagtttg gtgagcaaaa acactggatg atcgtcacgc tggcggcaat
     4921 gttggggcaa atcggcacac ccggcggcgg ttttggtctt tcttatcatt ttgccaatgg
     4981 tggtaacccc acgcggcgtt ctgcggtgct ctcttccatg cagggcagct tgcctggcgg
     5041 caccgatgcg gtagataaaa tccctgttgc ccgcattgtt gaagcactgg aaaaccctgg
     5101 tggcgcatat caacacaacg gtatgaaccg acatttcccg gatattcgtt ttatctggtg
     5161 ggcgggcggt gccaacttta ctcatcatca ggataccaat cgcctgatcc gtgcctggca
     5221 aaaaccggag ctggtggtga tctctgaatg cttctggacg gctgcggcga aacacgcgga
     5281 tatcgttctg cctgcgacca cttcgtttga gcgtaatgat ctcaccatga ccggcgatta
     5341 cagcaatcag catctggtgc cgatgaaaca agtggtgccg ccacgttatg aagcgcgtaa
     5401 tgattttgat gtctttgccg agttgagtga acgttgggag aagggcggct atgcgcggtt
     5461 tacggaagga aaaagtgagc tgcaatggct ggaaacgttt tataacgttg cccggcagcg
     5521 cggggcaagc cagcaggttg aattgtcgcc atttgctgag ttctggcaag ccaaccagtt
     5581 aattgagatg ccggaaaacc cggacagtga gcggtttatt cgcttcgccg attttcgccg
     5641 cgatccgcag gcgcatccat taaaaaccgc cagcggtaag attgaaatat tctcgcagcg
     5701 tattgccgat tatggttacc aggattgccc tgggcatccg atgtggctgg agccggatga
     5761 gtggcagggc aatgccgaac cagaacagtt gcaggtactt tctgctcatc cggcacatcg
     5821 tctgcacagc cagctgaatt acagttctct gcgcgaattg tacgcggtgg caaatcgtga
     5881 gcctgtcacc attcatcctg acgatgccca ggcgcgcggc atacaagatg gcgatattgt
     5941 tcggttgtgg aacgcacgcg ggcaaattct tgccggagcg gtcattagcg agggaattaa
     6001 acctggcgtg atttgtattc atgaaggggc atggccggat ctggatttaa ccgctgacgg
     6061 tatttgtaaa aacggcgcgg tgaacgttct gaccaaagat ctccccagct cgcggctggg
     6121 gaatggctgt gcgggtaata cggcactggc atggctggaa aaatacaacg gtccggaact
     6181 gacacttaca gcgtttgaac caccggccag ctcataatcc aggtgggtag ttgggtttca
     6241 tcctgccatg cgcagtcgac aatgtgaaaa ccctgcgcat ggtaaaaatc tatcgccggt
     6301 tgattttttt gataaacctc cagcatcaga tggggatggc gctgctgcac atactgcatt
     6361 agcgccttac caataccgcg tctgacggcc ttcggtgcga caaacattgc agccagaaat
     6421 cggccttcca taatgctgac aaaaccgaga agcttaccgt cttcttccca gacccagttt
     6481 tgcgcattgg caagataggc atcccgcacc agcggaatgc aatcacgcca gtaattcgct
     6541 tttataaagg gatgccccca ggttgtactt tccagccaca gttcgaggat cgcggggagt
     6601 tctgaacgtt gcgcttcccg aatcatggtt tatttcccgg atagcaacag cagccaacca
     6661 catgatcatt caccagccca catgcctgca taaaggagta acagattgtg gtaccaacaa
     6721 acttaaaacc acgctttttc agggctttag atagagcgtc ggaggcgggc gtagatgtag
     6781 ggatttcgct caacgttgtg gcttgtgtca cctgtggttg atgattgaca aacgaccaga
     6841 caaagtcggc aaacggttcg ccgttctgtt ccatttgcag gtacgcccgc gcattaccaa
     6901 taattgcctg aattttcccg cgatggcgga taatcccggc gtcctgtacc agtctttcga
     6961 catcctcttc ctgcattgca gccaccttca ccggatcgaa ctgatgaaag ctggcgcgat
     7021 agttttcgcg ttttttgagg acggtgatcc acgataatcc agcctgctgc ccttcaaggc
     7081 agatcatttc gaacagtttt ttactgtcag tttcaggcac gccccactca ttatcatggt
     7141 aggcaatata aagcgggtcc tgactcaccc agccgcaacg ttccatactt tccctcgcta
     7201 tgtacccgat cgtaaaaatt tcagccgctt ttcttgacgc atcggtgaaa aagtcaatat
     7261 ttaatacagg gctactatgc ccgatatcct tcatacaatg acaataatat tgccgaattc
     7321 ggctcttccc tggagtcgtg ttaatgatca taaaaaaaag cggtggtcgc tggcagctaa
     7381 gcctgctggc gagtgtggta atcagtgcct tttttctcaa cacagcttac gcctggcaac
     7441 aagaatatat cgttgatacg caacccggac attccacaga gcgttacacc tgggatagtg
     7501 atcatcaacc tgattacaac gatattttgt cgcaacgtat tcaaagtagc caaagggcgc
     7561 tgggactgga agtcaatctg gcggaagaaa ctcctgtgga tgtgaccagc agtatgagta
     7621 tgggctggaa ttttccttta tatgagcagg ttacaaccgg cccggtcgcg gcattacatt
     7681 acgatggcac aaccacctcg atgtataacg agtttggcga cagtactacc acgctggccg
     7741 atccgttatg gcatgccagc gtgagtacct taggctggcg tgttgactcc cggcttggcg
     7801 atctccgacc ctgggcgcaa atcagctata accagcaatt tggcgagaat atctggaagg
     7861 cgcaatcagg cctgagccgg atgacggcga caaaccagaa cggcaactgg ctggatgtca
     7921 ccgtaggcgc tgatatgttg ctcaatcaaa atattgctgc ctatgctgcg ctatctcagg
     7981 cagaaaatac cactaacaat agcgactatc tgtatacgat gggggttagt gccagatttt
     8041 aacgttatag atacaattga aaccattgga taacaacagt tgtggcgata gtgggtgcta
     8101 acttactaaa taataaattt ggtgaataat tgtcgagtca ttcattcctg aactaatgca
     8161 tttcattccg ttctgatggc atttcatgcc gtttttcccc aggcataaag tgcacttcgt
     8221 tatggttgtc agcagagatt tttccttttt attactgcag gaatactgcc atgacacctt
     8281 caaattatca gcgtacccgc tggttgacac tcatcggtac tatcattacc cagtttgcgc
     8341 tggggtcggt ttatacctgg agcctgttta acggcgcgct ttctgccaag ctggatgcgc
     8401 cggtaagcca ggtcgctttc tctttcggct tgttaagtct ggggctggca atttcatctt
     8461 ctgttgcggg gaaattacag gaacgttttg gcgttaaacg cgtcactatg gcttccggca
     8521 ttttgctggg attaggcttc ttcctgacag cgcattctga caacctgatg atgctgtggt
     8581 taagcgccgg tgtgctggtc ggtctggcgg atggcgcggg ttacctgctg acgctctcta
     8641 actgcgtgaa gtggttcccg gagcgtaaag gtctgatctc cgcgttcgct atcggttctt
     8701 atggtctggg tagcctgggg ttcaaattta tcgacacgca gctgctcgaa acggtgggtc
     8761 tggaaaaaac atttgtgatt tggggagcga ttgcgctggt gatgattgtt ttcggcgcaa
     8821 cgttaatgaa agacgcacca aaacaggaag tgaaaaccag caatggtgtc gtggagaaag
     8881 attacacgct ggcagagtcg atgcgtaaac cgcagtactg gatgttagcg gtaatgttcc
     8941 tgactgcctg catgagcggc ctgtacgtga ttggggtagc gaaagatatc gcccaaagtc
     9001 tggcacacct tgatgtggtt tccgcagcca atgcagtcac tgttatttcc atcgccaacc
     9061 tttcaggtcg tctggttctg gggatactgt ctgacaaaat cgcccgaatc cgtgttatca
     9121 ccattggtca ggtgatttcg ctggtgggta tggcggccct gctgtttgca ccattgaatg
     9181 cagtgacgtt ctttgcagcg attgcctgcg tggcgtttaa ctttggcggc actatcacgg
     9241 tgttcccgtc actggtcagt gagttcttcg gcctcaataa cctggcgaaa aactacggtg
     9301 tgatttatct cggtttcggt atcggcagca tttgtgggtc gattatcgcc tcactgtttg
     9361 gcggcttcta tgtgaccttc tacgtgattt tcgccctgct gattctgtca ctggcgctct
     9421 ctacgacgat tcgtcagcca gagcagaaaa tgttgcgtga ggcgcatggc tccctttaat
     9481 catctgccgc gagctactgg gctttagcct tagtgtgaat caaacgcttc aacgcaatgt
     9541 ataaacaatg acgcgtaaaa tttgggccag ttaactggcc ctttttactg ctttttgtaa
     9601 cgctaaagtc tcttttcaaa cttgcatttt tgtaaatttg tgcttcgtgc acactctttc
     9661 cccacacttt ttccctttgc tgtggtctac ttattcgcgc gtgtagattt tacttatctg
     9721 actacctccg cacttttccc ctgccgggcc tgaaaagccg ttaagcaggg tgttatcacc
     9781 tgtttgtcca gggtttgttt gcatgagata catcaaatcg attacacagc agaagctgag
     9841 ctttttgctt gcaatctata ttggcctttt tatgaatggc gcggtttttt accgccgctt
     9901 cggcagctat gcgcacgatt ttaccgtctg gaaaggcatt tctgctgttg ttgaactggc
     9961 cgccaccgta ctggtgacct tctttttact gcgtcttctt tcgctgtttg gccgccgcag
    10021 ctggcgtatt ctggcatcgc tggtggtgct cttttccgca ggtgccagct attacatgac
    10081 cttccttaat gtagtcattg gttatggcat catcgcatcc gtcatgacca ccgatatcga
    10141 cctgtcaaaa gaagttgttg gtctgaactt tattctctgg ttaatcgccg ttagtgcatt
    10201 gcctcttatc cttatctgga ataaccgctg tcgctacacc ttgctccgac aactgcgaac
    10261 cccagggcag cgtattcgca gcctggcggt cgtcgtactg gcgggtatta tggtttgggc
    10321 accgattcgt ttgctggata tccagcagaa gaaagtggag agggcgaccg gcgttgattt
    10381 gccgagttat ggcggtgtcg tagcgaactc ttatctgcca tcaaactggc tttctgcgtt
    10441 ggggctgtat gcctgggcgc gggtcgatga atcttccgat aacaattcat tgcttaatcc
    10501 ggcgaagaaa ttcacttatc aggcaccgca aaacgttgat gacacttatg tcgtgtttat
    10561 catcggtgaa accacgcgtt gggaccatat gggcattttc ggctatgagc gtaataccac
    10621 gccgaagctg gcccaggaga aaaatctggc ggcgttccgt ggctattctt gtgacacggc
    10681 gaccaaactt tctctgcgtt gcatgtttgt acgagagggt ggagcggaag ataatccgca
    10741 gcgcacatta aaagaacaga acattttcgc ggtgctgaag cagttaggat tcagttctga
    10801 cctctacgct atgcagagcg aaatgtggtt ctacagcaac acgatggcag acaacattgc
    10861 ttatcgtgag cagattggtg cggagccacg taaccgtggc aagccggtag atgatatgtt
    10921 gctggtagac gaaatgcagc aatcgctggg gcgcaacccg gatggcaagc atctgatcat
    10981 tttgcatacc aaaggttcgc actttaacta cacccagcgt tacccgcgca gctttgcgca
    11041 gtggaagccg gaatgtattg gtgttgatag cggctgtacc aaagcgcaga tgatcaactc
    11101 ctatgacaac tcggtgacct atgtggatca ctttatctcc agcgtgattg atcaggttcg
    11161 cgataagaaa gcgattgtgt tctacgcagc tgaccacggt gagtcaatta atgaacgcga
    11221 gcacctgcac ggcacaccgc gtgaactggc accgccggag cagttccgtg tgccgatgat
    11281 ggtctggatg tcagataaat atctggaaaa tccggtcaat gcacaggcat ttgcgcagct
    11341 gaaaaaagaa gccgacatga aagtgccacg ccgtcatgta gaactgtacg acaccatcat
    11401 gggttgtctt ggctatactt caccggatgg tggaattaac gaaaacaaca actggtgcca
    11461 catcccgcag gcaagagagg cggcggctaa ctaagaggcc tgctgacttt ctcgccgatc
    11521 aaaaggcatt ttgctattaa gggattgacg agggcgtatc tgcgcagtaa gatgcgcccc
    11581 gcattcggtg attggcgcag cctggtagcg cacttcgttc gggacgaagg ggtcggaggt
//
LOCUS       DAOTRK010000145        11540 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.146, whole genome shotgun sequence.
ACCESSION   DAOTRK010000145 DAOTRK010000000
VERSION     DAOTRK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.146"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..219)
                     /locus_tag="QIE41_003837"
     CDS             complement(<1..219)
                     /locus_tag="QIE41_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDS3532673.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYR"
     gene            complement(206..382)
                     /locus_tag="QIE41_003838"
     CDS             complement(206..382)
                     /locus_tag="QIE41_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532674.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            678..1334
                     /gene="uvrY"
                     /locus_tag="QIE41_003839"
     CDS             678..1334
                     /gene="uvrY"
                     /locus_tag="QIE41_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDS3532675.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            1331..3163
                     /gene="uvrC"
                     /locus_tag="QIE41_003840"
     CDS             1331..3163
                     /gene="uvrC"
                     /locus_tag="QIE41_003840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDS3532676.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            3220..3768
                     /gene="pgsA"
                     /locus_tag="QIE41_003841"
     CDS             3220..3768
                     /gene="pgsA"
                     /locus_tag="QIE41_003841"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDS3532677.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            3920..3995
                     /locus_tag="QIE41_003842"
     tRNA            3920..3995
                     /locus_tag="QIE41_003842"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3953..3955,aa:Gly,seq:gcc)
     gene            4050..4123
                     /locus_tag="QIE41_003843"
     tRNA            4050..4123
                     /locus_tag="QIE41_003843"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4082..4084,aa:Cys,seq:gca)
     gene            4136..4222
                     /locus_tag="QIE41_003844"
     tRNA            4136..4222
                     /locus_tag="QIE41_003844"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4170..4172,aa:Leu,seq:taa)
     gene            complement(4475..5206)
                     /locus_tag="QIE41_003845"
     CDS             complement(4475..5206)
                     /locus_tag="QIE41_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532678.1"
                     /translation="MERLLDAYKRILQEVDAQSFNLNEDKYSGVFLPVPFEEYWHSPV
                     KIMLVGRETAGWNTLNGKNTISRVLGLIPDVTIGQVVEEAVDRYRKHLPAQNDGTTNH
                     KSRSRFTQYHFRLARELNIPPQAIVYANLLAWDYDGLPPLNRPQNEVQEVILASLKLL
                     AVQIKHLEPDFTIFASGARRTDYIIKQVLTELGGYETSSVIPGKLWEFKTGHTICFRI
                     AHPRAMRGHQKYRDEVIARIKQLCA"
     gene            5287..5970
                     /locus_tag="QIE41_003846"
                     /pseudo
     CDS             5287..5970
                     /locus_tag="QIE41_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172378.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
     gene            6189..7517
                     /locus_tag="QIE41_003847"
     CDS             6189..7517
                     /locus_tag="QIE41_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDS3532679.1"
                     /translation="MKIANIRLTNFRCFDEFSVDFDPRLTVLIARNGRGKSTLLDAVA
                     LALGPFLTRLPGIKGLSFKKTDFRIDENGDQPSYMRVSCTSTTGITWDRTERRDLTKK
                     TLGSIPQGAALKALNDYVDTFIDSHNENKHYTLPIFVYYGTGRGVFDIPLRKRAFNKH
                     FSRFEALDGALESKTNFKRFVQYFYFLEEKENRLQKEQRSFEVEIPELRAIRLAVEKL
                     LPEFSNIRSAVPAGIMVDWCKPIGNGETTIQKLRIEQLSDGYRTTLAMVMDIAARMAE
                     ANPYSSDPLSSEGIILIDEVDLHLHPEWQREFLPRLINVFPHLQFIVSTHSPFIIQSV
                     KEGMLVDLDKDDISDSPSLNKELSIEDIAEGVMGMEKVQRSELFNKQVAVSDRYYQLL
                     NEGRSESDPEVRKLSAELDKLEEFFGDNPAYVALLRAERRKIMGTQGSKQ"
     gene            7514..8125
                     /locus_tag="QIE41_003848"
     CDS             7514..8125
                     /locus_tag="QIE41_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDS3532680.1"
                     /translation="MRPVNRPPYNGTVYADYKSFLPSLLTAYGGYCSYCERPDKVDVE
                     HVVPKTHAPLLITDWNNFLLGCPRCNRDFKKSKNTQRIGYVWPDVENTFNLLTYHPDG
                     RVEPKQGLSAGLTAQVQATIDLVCLDDGNQVQKTLNLGRRRYFGLANKAKTLYLSNLL
                     SIDEVMDMAQLGYWSIWYNVFQGVPAVLSALENSYPNTAINRP"
     gene            8551..9216
                     /gene="yecA"
                     /locus_tag="QIE41_003849"
     CDS             8551..9216
                     /gene="yecA"
                     /locus_tag="QIE41_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDS3532681.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERLEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(9278..10489)
                     /gene="tyrP"
                     /locus_tag="QIE41_003850"
     CDS             complement(9278..10489)
                     /gene="tyrP"
                     /locus_tag="QIE41_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDS3532682.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVISVQF
                     LIAAGLLPEVG"
     gene            10679..10918
                     /locus_tag="QIE41_003851"
     CDS             10679..10918
                     /locus_tag="QIE41_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDS3532683.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     DGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            complement(10956..11453)
                     /gene="ftnA"
                     /locus_tag="QIE41_003852"
     CDS             complement(10956..11453)
                     /gene="ftnA"
                     /locus_tag="QIE41_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDS3532684.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
BASE COUNT         3113 a         2592 c         2848 g         2987 t
ORIGIN      
        1 acggtaggct tgtttaattt gcttaaccgt tttggaaaat actgcagcct gggtttcgtc
       61 ttcgatcagc gcaagctgtt tttccatctt taacatcacg cggcccgcgt cagcttgtcc
      121 catcgcctgc agcatcagcg tcagcatcgc tttcaggcag gagacttcat ttgccagttc
      181 ttgattattc tcggcagtag aaaaatcagg cgtactcatt tattttcctc gtcatgttgc
      241 aatgaaaatt tgcggtgaaa aatgttaagg cggcggagta taccataagc tttgctaaaa
      301 atagcagtgg ttgttttttg agcgtgatat cggcagtgct ataaaatacg ttaatataat
      361 gaccaataaa tatttttatc atgaatgttt tttgcccgta ttgttcgggt taattaatgt
      421 tacatattca gcgggctgat tttcattttt gctgaataaa gtcaattttt gtcacatttc
      481 atcgtagggc ttactgtgaa acgatcccgg aagccgttgg tgacgggcgt gaccataact
      541 gtggacaatc gaatttacaa aaacgagaga aaaatcgaat acccaccatt tttaacgttt
      601 caaagttgca ataaaaaccg ctaatatacg aatgactaac tatcagtagc gttatcccta
      661 tttctggaga tattcctttg atcaacgttc tacttgttga tgaccacgaa ctggtgcgcg
      721 cagggatacg acgcattctg gaagatataa agggtataaa agtcgtcggt gaggcatcgt
      781 gcggtgaaga cgccgttaag tggtgccggg caaatgccgt tgacgtggtg ctaatggaca
      841 tgagtatgcc gggcattggc ggtcttgagg cgacgcgtaa aatcgcgcgt tccacagctg
      901 atgtcaaaat catcatgctt accgtccata cagaaaaccc tttaccagcg aaagtcatgc
      961 aggccggtgc tgctggctac ctcagcaaag gcgcggctcc gcaggaagtc gtgagtgcga
     1021 tccgttctgt ctattcaggg cagcgttaca ttgcttctga catcgctcaa caaatggcgt
     1081 taagccagat cgaaccagaa aaaacagaaa gcccatttgc cagtttgtct gaacgtgaat
     1141 tgcagattat gctgatgatc accaagggcc agaaggtcaa tgagatctca gaacagctca
     1201 atctcagtcc gaaaacggtg aacagctacc gctatcgtat gttcagtaaa ctaaacattc
     1261 atggcgatgt tgagctgact cacctggcaa ttcgccatgg tctgtgtaat gcggagacat
     1321 tatcaagtca gtgagtgatc agtttgacgc aaaagcgttt ttaaaaaccg taaccagcca
     1381 gccaggcgtt tatcgcatgt acgatgctgg tggtacggtt atctatgtcg gcaaagcgaa
     1441 agacctgaaa aaacggcttt ccagctattt ccgtagcaac ctcgcttcgc gcaaaaccga
     1501 agcgctggtc gcccagatcc agcaaattga tgtaacggtt actcatacag aaaccgaagc
     1561 gctgttgctg gaacacaact acatcaaact ctatcagccg cgttacaacg ttttgctacg
     1621 cgatgataaa tcttatccct ttatcttcct gagtggcgat acccatccgc gtctggcgat
     1681 gcatcgtggt gcgaagcatg ccaaaggtga atatttcggc ccgttcccga atggctatgc
     1741 tgtacgtgaa acactggcgc tactgcaaaa gatattcccc attcgccagt gcgaaaatag
     1801 tgtttatcgc aatcgctcgc gtccgtgtct gcaatatcag atagggcgct gtctgggacc
     1861 gtgcgttgaa ggactggtga gtgaagaaga atacgctcag caggtcgagt atgtgcgcct
     1921 gtttttgtct ggcaaagatg atcaggtgct tacgcaactc attagtcgta tggaaactgc
     1981 cagccagaat ctggagtttg aagaagcagc acgtattcgc gaccaaattc aggcggtgcg
     2041 acgcgtcacc gaaaaacagt ttgtgtccaa taccggcgac gaccttgacg ttattggtgt
     2101 ggcgttcgat gcgggcatgg cttgtgtcca cgtattgttc attcgtcagg gtaaagtgct
     2161 cggcagccgc agctatttcc cgaaagtgcc tggcggtacg gaactgagcg aggtggtgga
     2221 aaccttcgta ggtcagttct atttacaagg cagccagatg cgcaccttac cgggtgagat
     2281 cctgctcgat tttaatctta gcgataaaac gctgctcgcc gattcccttt cagaactggc
     2341 gggacgcaag attaatgttc aaaccaaacc tcgcggcgat cgggcgcgtt atctgaaact
     2401 cgcgcgcacc aatgcggcga cggccttaac cagcaaactt tcgcagcaat ctaccgttca
     2461 ccagcgactg accgcgcttg ccagcgtgtt gaaattaccg gaagtgaagc ggatggagtg
     2521 ctttgacatc agccatacca tgggtgaaca aaccgtcgct tcctgtgtgg tgtttgatgc
     2581 taacggcccg ctgcgtgcgg agtatcggcg ctataacatt actggcatca cgccgggcga
     2641 tgattatgcg gcgatgaatc aggtgctgcg tcggcgttat ggtaaagcca ttgacgacag
     2701 taagatcccg gatgtgatcc ttatcgacgg cggcaaaggc cagcttgcgc aggcgaaaaa
     2761 tgtcttcgcc gaactggatg tctcatggga taaaaatcat ccgctgctac ttggcgttgc
     2821 caaaggtgca gataggaagg ctgggctgga aacgctgttc tttgagccgg aaggtgaggg
     2881 attcagtttg ccgccagatt ctcccgcgct gcatgttatc cagcatattc gcgatgaatc
     2941 acacgatcac gcgattggcg ggcaccgtaa aaaacgggcg aaggtcaaaa ataccagttc
     3001 cctggaaacc attgaaggcg tcgggccaaa acgtcggcaa atgttgttga aatatatggg
     3061 cggtttgcaa ggtttacgta acgccagcgt cgaggaaatt gcaaaagtgc cgggtatttc
     3121 gcaaggtctg gcagaaaaga tcttctggtc gttgaaacat tgatgtctct gtagcaacat
     3181 aggggtaatc ttactgacaa cagatagtta cccgtcatta tgcaatttaa tatccctacg
     3241 ttgcttacac tgttccgtgt catccttatc ccattctttg tattggtctt ttatttgcct
     3301 gtcacctggt cgccgtttgc cgccgcgctc attttctgcg tcgcggcggt gactgactgg
     3361 ttcgatggtt ttctggcacg ccgctggaac cagagtaccc ggtttggtgc tttccttgac
     3421 ccagtagcag ataaagttct cgtggctatc gccatggtgc tggtaaccga gcattaccac
     3481 agctggtggg tgaccttacc ggcagcaacg atgatcgccc gtgaaattat tatttctgcg
     3541 ctacgcgaat ggatggcgga gttgggtaaa cgcagtagcg tggctgtctc ctggattggg
     3601 aaagtgaaaa ccactgccca gatggtagcg ctggcctggc tgctgtggcg tccgaacatt
     3661 tgggttgagt acgccggtat tgcacttttc tttgtggctg cggtactgac tctgtggtca
     3721 atgttgcaat atttgagcgc tgcgcgtgca gatttgcttg atcagtgatc gtttcggcgt
     3781 aattttcagc aaacgatcaa aagtggtgaa aaatatcgtt gactcatcgc gccaggtaag
     3841 tagaatgcaa cgcatcgaac ggcggcactg attgccagac gataataaaa tcaagtgatt
     3901 aactgattgc ttgatgaatg cgggaatagc tcagttggta gagcacgacc ttgccaaggt
     3961 cggggtcgcg agttcgagtc tcgtttcccg ctccagttta aaagacatcg gcgtcaagcg
     4021 gatgtctggc tgaaaggcct gaagaatttg gcgcgttaac aaagcggtta tgtagcggat
     4081 tgcaaatccg tctagtccgg ttcgactccg gaacgcgcct ccactttctt cccgagcccg
     4141 gatggtggaa tcggtagaca caagggattt aaaatccctc ggcgttcgcg ctgtgcgggt
     4201 tcaagtcccg ctccgggtac catgggaaag ataagaataa aatcaaagca ataagcagtg
     4261 tcgtgaaacc accttcgggt ggtttttttg tgcctgaaat tcacgccctg tgcatatcct
     4321 gtgctcacgg ggggcaatga gctgcaatct caagcccggc aatgccgaat cgaacgtgtc
     4381 gcctccagca tagagaacca ggccgctttg cctgttttcc ggtactcttc cgggaagggg
     4441 ggccagctga gcaagctata atgctctggc caccctaagc acaaagttgc ttaattcgtg
     4501 caatcacctc atctctgtat ttctggtgtc cgcgcatagc ccttggatgg gctatcctga
     4561 aacagattgt atgacctgtt ttgaactccc atagcttccc cggaatgact gaagaagtct
     4621 catagccgcc tagctcagta agcacctgtt ttatgatgta gtctgttctc cgggctccgg
     4681 atgcaaagat ggtgaaatca ggctcaaggt gtttaatttg taccgccagc agtttcagcg
     4741 aggctaatat gacttcctgc acttcatttt gagggcgatt gagagggggc agtccgtcgt
     4801 aatcccacgc cagtagattg gcatacacga tagcctgagg aggaatatta agctcccggg
     4861 ccagccggaa gtgatactgc gtgaagcggc tacgtgattt gtggttcgtg gtgccatcat
     4921 tttgtgccgg aagatgtttc ctgtaacgat ctacagcctc ttcaaccacc tgcccgatgg
     4981 tgacgtcagg tataagtccc agaacccgtg atatcgtatt cttaccattc agcgtattcc
     5041 agccagcggt ttcacgccca accagcatga tttttaccgg tgagtgccag tattcttcaa
     5101 agggcacggg taaaaagacg cctgagtatt tatcctcatt caggttaaat gactgggcgt
     5161 caacttcctg taatatccgt ttgtatgcgt ctaatagtct ttccattctt caatcctgcc
     5221 ccgtaataaa aataccttca gatatatatt attttcatga tatcaatcag atacatgagg
     5281 ataaatatgc aaaaatcctt ccgattcacc aattcatcca tcaaagccct tccggcaaac
     5341 acagataccc gatctacaga gcttgaggta tctgataccg aagtcattgg cctcaaatgc
     5401 ctctcaggtc gaacgggcaa caaacgtttc ctcctgcgct acacattcca cggtaccaaa
     5461 aaaagtatca gtattggcag attccctgaa atcgatgtgg gaacagcccg tcagatagct
     5521 cgtcgtcaca aggaggcgat tgcactggga aatgacccta aggcagagcg cgataattat
     5581 cgggcgatgc caacgcttag tgagtttttt caccagacct atgttccgtt catcaagcgc
     5641 cataaaaagt cctgggataa ggatgttcag cgtttcacgc aatacattga acctcgtctg
     5701 gggaagatac gttactgcga ccttcaggcc cgggaaatcc agcaggtgct ttttgacatg
     5761 cttgagggcc gtattcacgg ccagcagtat gcaccgtcta cctgtaacag ggcactggct
     5821 attctgaaaa ccatgggccg ttatgcgcta cgacaggatg tcctggagaa gaatgaagcc
     5881 gataagatcc ctttgctgaa ggagaataat caccggacgc gcttttttga cgctgatgag
     5941 atcaggagaa tccttgatgc tgcgtctcga taacccaata aagctgccgg agggctgacg
     6001 ccggaagagt cagagaacag ataccatttt tactgtaaaa ccgtggccag tattacttga
     6061 ccactacatt ctaaggcttg ctttaatgct aaggctaaat gattttgata tagtaagttc
     6121 atacttagcg ggcagatgcc cacagacata gtaccgaaaa taaatcaggt attctgcgag
     6181 atttcagtat gaaaatagcc aatattagac ttacgaattt tagatgtttt gacgagttta
     6241 gtgttgattt tgacccaaga cttacagttc tcattgctcg taatggtaga gggaaatcta
     6301 ctctgcttga cgcggtagca ttagctttag gaccattttt aactcgtttg ccagggatca
     6361 aagggctaag tttcaagaaa accgatttcc ggatcgacga aaatggagac cagccgtcct
     6421 atatgcgagt aagttgtact agtacaacgg gtataacttg ggatagaact gagcgaagag
     6481 atctgacaaa aaaaacgtta ggtagcatcc cccaaggggc agcccttaaa gccttgaatg
     6541 attacgtcga tacgttcatt gattctcaca atgagaataa acactataca ttgccaattt
     6601 ttgtttatta cggaactggg cgtggagtgt ttgatatacc actcagaaaa cgtgcattta
     6661 acaagcattt ttctcgattt gaggcccttg acggtgctct tgaaagcaaa acaaatttca
     6721 aaagatttgt gcaatatttt tattttcttg aagaaaaaga aaatagatta cagaaagagc
     6781 aacgatcatt tgaagtagaa atccctgaat taagggcaat acgcttggct gtggaaaaat
     6841 tactgccaga gtttagcaat attcgctcgg cagtacctgc tggaattatg gtcgattggt
     6901 gtaagcctat tggaaatggt gaaacaacca tacaaaagct aaggattgaa caacttagtg
     6961 atggctatag aacaacttta gctatggtta tggatattgc agcaagaatg gcagaagcta
     7021 acccctattc gagtgatcct ctaagttctg aggggataat tctcattgat gaggtagatc
     7081 tgcatctgca tccggaatgg caacgtgagt ttctccctag attaatcaac gttttccctc
     7141 atcttcagtt catagtatcg acgcattcac catttatcat ccagtctgtc aaagagggga
     7201 tgttggtcga tcttgataaa gatgacattt cggatagccc ctctttgaat aaagaactca
     7261 gcatcgaaga tattgctgag ggtgtaatgg gaatggaaaa agttcaacga agtgaattgt
     7321 ttaacaaaca agtcgcagtt tcagatagat attatcagtt actgaatgaa ggaagaagtg
     7381 aaagcgaccc agaggtaagg aagctctccg ctgagcttga taaattggaa gagttttttg
     7441 gagataaccc agcttatgta gccttacttc gtgctgagcg ccgaaaaata atggggacac
     7501 aaggaagtaa acaatgagac ctgtaaatcg acctccatat aatggaactg tttatgcaga
     7561 ttataaatca tttttacctt cgctattaac tgcatatggc ggatattgtt cttattgtga
     7621 gcggccggat aaggttgatg tggagcatgt tgtcccaaaa acgcatgcac cacttttgat
     7681 tactgattgg aacaattttt tactgggttg ccctcgctgt aaccgagatt ttaaaaaaag
     7741 taagaatact caacgtatag gctatgtttg gccagatgtt gagaatacat ttaatttatt
     7801 gacataccat ccagatggta gagtagagcc taagcagggg ctgtctgccg gactaactgc
     7861 gcaggtacag gctactattg atttggtatg tcttgatgat ggtaaccaag tacaaaaaac
     7921 tttaaatctt ggtagaagga gatattttgg actcgcgaat aaggccaaga ctctttatct
     7981 gagtaattta ttgagtattg atgaggtcat ggatatggct cagttaggat attggtcgat
     8041 ctggtataat gtgtttcaag gtgtacctgc tgtcctttca gctttggaga attcctatcc
     8101 taataccgct ataaaccgcc catgacatag gtgaaatgaa ttggctcagg tgcgtacaag
     8161 gggaagactt acctgtgcag cttctgtgtt caacgttatc catgtctatc tatttactgc
     8221 caattacagt aaagttggtg gataattaca gcaaagtgtt gcaatgggtt gctatgtaaa
     8281 tcattgatcc tgcgtagaat ttaaaatccc tcggcgttcg cgctgtgcgg gttcaagtac
     8341 ccgctccggg taccatggga aagataagaa taaaatcaaa gcaataagca gtgtcgtgaa
     8401 accaccttcg ggtggttttt ttgtgcctgc aacttgtcgt tacaccctcc ttaattttta
     8461 atcaccggca aagccactca aaaagtatct ctgttacgga ccgacatgta agataggtgc
     8521 tggcgagttg agatccacaa ggaaaagcgt atgaaaacgg gaccgttaaa cgaaagtgag
     8581 ttggaatggc tggacgatat tctgaccaaa tacaacactg accacgccat tcttgatgtg
     8641 gcggagctgg acggtttatt gacggcggtg ttgagttctc cgcaagagat tgaaccggca
     8701 cagtggctgg ttgccgtgtg gggtggggct gactatgtgc cacgctgggc gtcagagaaa
     8761 gagatgacgc gctttatgaa cctggctttt cagcatatgg ctgataccgc agagcgtctg
     8821 aacgaattcc cggagcagtt tgagccgtta ttcggcttgc gagaagtcga tggcagcgag
     8881 ctgacgattg ttgaggagtg gtgctttggc tatatgcggg gcgtggcact ttctgactgg
     8941 tcaacgttgc ccgattcgtt aaaaccagcg ctggaggcga ttgcgctgca cggtactgag
     9001 gaaaacttcg agcggttaga gaagatgtcg ccagaagcgt ttgaagagag cgtagatgcc
     9061 attcgactgg cggcgcttga tctacatgcg tactggatgg cgcatccgca ggaaaaagct
     9121 gttcagcaac cgatcaaagc agaagagaaa ccggggcgta atgatccttg cccgtgcggt
     9181 agtggtaaga aatttaagca gtgctgcctg cattaagaga tcaaattggg tgtcagcgtt
     9241 aattctggca cccaataagg aatttgaggc tatctgatca ccccacttct ggtaacaagc
     9301 ctgctgcaat caaaaattgc acgctaatca cagcaatacc acagagaaac accaccacca
     9361 gcgccggacg accacctttc acccggtaac ccgcctgagg attgtgcttt ctgctttgcc
     9421 aggtcaacag cgaagggata atcaatgcca gtaccgccag cgccacaccg gcgtaaccca
     9481 gcgccatcac aaatcctcgt ggataaaaca gtgcaaacgc caacggcggc agaaaggtaa
     9541 ttgcaccagt ttgcaatcgt ccaccaacgg tatttgaacg ctgaaacaaa tctgccagat
     9601 aatcaaataa acctaacgca acgccgagaa atgacgtggc gagggctaaa tcagcaaata
     9661 aatgcaccgc cagctcaaca tgcggagagg ccaccatttc gcgtaatgcc tgtaacagcc
     9721 cgtttaatcc ggcatgatta gccagcaatc ccataaaggt tgttgaatca atgctgccaa
     9781 gcgtcgccac ctgccagaaa atatatgcca ccagggggat cgcactaccg attataaaca
     9841 cccagcgtag cttacgaatg ttgccatcca tatagctgac aatactcggc acgctaccgt
     9901 gaaaaccaaa cgacgtaaaa atcaccggga ttgcagatag tgcgagcccc tgttgcaacg
     9961 gcagggttaa aagattcacc ttatgaatat gcggaagtag cagtaccagc atcaccacca
    10021 gaaaaataat tttggcgctg aacagaaaac ggttaaataa atcgaccagc gaagttccga
    10081 cacaaaccac gccaccggca acaaaagtga acaacagcac gccagcggtt gccgacattg
    10141 aaatacctgt ccagtcgctg atgctggaag ccaacaattc accagcaccg ctgatgtatg
    10201 ccgcagtcag agcatacatt aagaacatca tactgaagcc cgtcagccac tgaccgtagc
    10261 gtcccagata gcgttttgcc agcgtgccaa gaccggtatc tgccggaaca tgctggtaca
    10321 cctccagcag taatagcgcc gtgtagcaca tcaacgccca aagcccaatt aacaagatta
    10381 acgtgacgct aaaaccaaca cctgccgcag ccagcggcat tgccagcatg cctgcgccaa
    10441 ttgtggttcc cgccacgata aaaacacttc ccagagttct gtttttcacg ctttcttctg
    10501 tcctgacaat ctttatgagt gatatctgcg gcgcaggtta aggcataacg gctgcttcgt
    10561 caaaccgacg ttacatatag tgtaaatata aatgtacaat aaaagcgttt agttctgcgc
    10621 tggcgcaaag tcttcataag cggcaatcgc tacgctagcc tttttttgag gaggaacgat
    10681 ggactctatt cacggtcatg aagtgttaaa tatgatgatt gaatcaggtg agcaatatac
    10741 gcatgccagt ctggaagctg cgattaaagc gcgttttggt gaacaggcac gttttcacac
    10801 ttgctcggca gacgggatga cagcgggaga gctggtagcg tttctggcag caaaaggcaa
    10861 atttatacct tcggaagacg gtttttcgac cgatcagagt aagatttgcc gtcactgatt
    10921 tgaaacggcg gcagaaaacc tgccgcctga gagcattagt tttgtgtgtc gagggtagag
    10981 agttctttgt cgataaaata cagaccttcg ccgcttttgc ctgccaggct taatttatca
    11041 ataatcgatt tgaacagttt ctcttcttca tgctgctcag aaacatacca ttgcaggaaa
    11101 ttaaatgttg ggtagtcctg attggtcatt gcagcatgag ccagttcgtt aattttctgc
    11161 gtgattaatt gttcgtgttt ataggtttcc tggaataatt catcaagtga ggaatattca
    11221 gcaaacggag attcaacggt attaatacgc ggtaagttgc cggtatcagt cagataatca
    11281 aacagacgct gcatatgcgt catctcttcc tgggcgtggc ggcgcaggaa cgcggcagca
    11341 ccttcgaagg tatgatagct gcaccaggcg ctcatttgct gataaagcag tgaagagtac
    11401 agttccaggt tcatctgctc attaagtttt tcaatcattt ctggtttcag catgatagtg
    11461 ctccacaaag gttatatttg ttgctgtggc gtcactataa tttgttattt tattatttgc
    11521 aaaaggtaaa atataaactt
//
LOCUS       DAOTRK010000146        11152 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.147, whole genome shotgun sequence.
ACCESSION   DAOTRK010000146 DAOTRK010000000
VERSION     DAOTRK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.147"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            278..1663
                     /gene="dcuC"
                     /locus_tag="QIE41_003853"
     CDS             278..1663
                     /gene="dcuC"
                     /locus_tag="QIE41_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDS3532685.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1704..2384)
                     /gene="dpiA"
                     /locus_tag="QIE41_003854"
                     /gene_synonym="citB"
     CDS             complement(1704..2384)
                     /gene="dpiA"
                     /locus_tag="QIE41_003854"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDS3532686.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2353..4011)
                     /gene="dpiB"
                     /locus_tag="QIE41_003855"
     CDS             complement(2353..4011)
                     /gene="dpiB"
                     /locus_tag="QIE41_003855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDS3532687.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4390..5448
                     /gene="citC"
                     /locus_tag="QIE41_003856"
     CDS             4390..5448
                     /gene="citC"
                     /locus_tag="QIE41_003856"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDS3532688.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5463..5759
                     /gene="citD"
                     /locus_tag="QIE41_003857"
     CDS             5463..5759
                     /gene="citD"
                     /locus_tag="QIE41_003857"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDS3532689.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5756..6664
                     /gene="citE"
                     /locus_tag="QIE41_003858"
     CDS             5756..6664
                     /gene="citE"
                     /locus_tag="QIE41_003858"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDS3532690.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6675..8207
                     /gene="citF"
                     /locus_tag="QIE41_003859"
     CDS             6675..8207
                     /gene="citF"
                     /locus_tag="QIE41_003859"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDS3532691.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8211..8762
                     /gene="citX"
                     /locus_tag="QIE41_003860"
     CDS             8211..8762
                     /gene="citX"
                     /locus_tag="QIE41_003860"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDS3532692.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8737..9615
                     /gene="citG"
                     /locus_tag="QIE41_003861"
     CDS             8737..9615
                     /gene="citG"
                     /locus_tag="QIE41_003861"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDS3532693.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEEVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSITPITICATAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9665..11128
                     /gene="citT"
                     /locus_tag="QIE41_003862"
     CDS             9665..11128
                     /gene="citT"
                     /locus_tag="QIE41_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDS3532694.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
BASE COUNT         2584 a         2942 c         2811 g         2815 t
ORIGIN      
        1 ggcagattat tttctgagca cgcaaccgca gctaacacca ttgctattaa caaatttgca
       61 tcaatccgcc atcaatttac acacattatt atgtggtaat tgccaaacgc taaatatgcg
      121 ttttgttatc tatgtataaa aacagcaact tcaatgtctt aatggcagtt tttcttgatt
      181 ttaatcagca ttcaccgcca atttattggg catatttttt ccttaagttt taggaatttt
      241 ttattattta ctttggggcc tggagacagg aaaaattatg ctgacattca ttgaactcct
      301 tattggggtt gtggttattg tgggtgtagc tcgctacatc attaaagggt attccgccac
      361 tggcgtgtta tttgtcggtg gcctgttatt gctgattatc agtgccatta tggggcacaa
      421 agtgttaccg tccagccagg cttcaacagg ctacagcgcc acggatatcg ttgaatacgt
      481 taaaatattg ctaatgagcc gcggcggcga cctcggcatg atgattatga tgctgtgtgg
      541 ctttgccgct tacatgaccc atatcggcgc gaatgatatg gtggtcaagc tggcgtcaaa
      601 accattgcag tatattaact ccccttacct gctgatgatt gccgcctatt ttgtcgcctg
      661 tctgatgtct ctggccgtct cttccgcgac cggtctgggt gttttgctga tggcaaccct
      721 gtttccggtg atggtaaacg ttggtatcag ccgtggtgca gctgctgcta tttgtgcctc
      781 cccggcggcg attattctcg caccgacttc aggggatgtg gtgctggcgg cgcaggcttc
      841 cgaaatgtcg ctgattgact tcgccttcaa aacgacgctg cctatctcaa ttgctgcaat
      901 tatcggcatg gcgatcgccc acttcttctg gcaacgttat ctggataaaa aagagcacat
      961 ctctcatgaa atgttagatg tcagtgaaat caccaccact gctcctgcgt tttatgccat
     1021 tttgccgttc acgccgatca tcggagtgct gatttttgac ggcaaatggg gtccgcaatt
     1081 acacatcatc actattctgg tgatttgtat gctgattgcc tccattctgg agttcatccg
     1141 cagctttaat acccagaaag ttttctctgg tctggaagtg gcttatcgcg ggatggcaga
     1201 tgcgtttgct aacgtggtga tgctgctggt tgccgctggg gtattcgctc aggggcttag
     1261 caccatcggc tttattcaaa gtctgatttc tatcgccacc tcgtttggtt cggcgagtat
     1321 catcctgatg ctggtattag tgatcctgac aatgctggcg gcagtcacga ccggttcagg
     1381 caatgcgccg ttttatgcgt ttgttgagat gatcccgaaa ctggcgcact cttccggcat
     1441 taacccggcg tatttgacta tcccaatgct gcaggcgtca aacctcggtc gtaccctgtc
     1501 acccgtttct ggcgtagtcg ttgctgttgc cgggatggcg aaaatctcac catttgaagt
     1561 cgtaaaacgc acctcggtgc cggtgcttgt tgggctggtg attgttatcg ttgctacaga
     1621 gctgatggtg ccaggaacgg cagccgcggt cacaggcaag taaatagtca tgccggaggc
     1681 aagtttcctc cggctttatc atgtcaccca ctgtggtata tgcgttgcgg tctgccaact
     1741 ttgccgtgaa caatttcagc aataatcaga tggcggctgg cgcaatattc aagataacgt
     1801 ctggcagtgg tgcggctgat agtcagtgcc tgcgccaccg tttctgccgt atgttgcaca
     1861 ccaggctctt taaacagttt tcgcaccgcg tttagcgtca aggggtcaat gccggtcggc
     1921 agttcgtctt tgggttcacc gcgagcatag gcattaaaca tctcatcaat ttgcttctgg
     1981 ctggcgctat caatactttc cagcatatgt ttacgctggc ggaaacgggt tagcgtttgc
     2041 cccagccgtt cataggcaat tggcttaatg agataatcaa atacaccaca acgtacggct
     2101 tcagacaccg tttccatatc actggctgca gtggtaaaca ccacgtcgcc gggataatgc
     2161 gcctgcacca gttcatgcag taaattaatc cctctaccgt caggaagata gttatcgagc
     2221 aagattagcc ccggcttaaa acgctcgatc atcattcggg cctgcgccag gtttcccgcc
     2281 agtaatatct gactgaatcc gggaatgtga cgaatatatt ccgcatgcat ctctgccagc
     2341 ggcgtttcgt cctcaacgat caatagggtt aatggagctg tcattaggtt tcactttcgg
     2401 aatatagatt gaaaataagg taccgcaggg atcattatct tcgagagtga taacaccacc
     2461 gcagcgcgtt acgtagctgg caatcaagta caacccaatg ccatgttcgc cgggctcgtc
     2521 agcacgcgta ctgacaccct gctcaaatat tttgtctcgt agagactctg gaacgccgca
     2581 gccctgatcg gcgacttcaa tcaccacatc atcgccttca tcgctgagga ataattcaac
     2641 gatcttgttt ccttcatcgc tacgcaggct ggcttcgaag gcgttatcaa gtaaattgcc
     2701 aacaatggct gcaaactcgg tgctgtccag tcctggcggc agttgcgaaa gctgacttcc
     2761 ggggacgatg accattttta gccccagttc gcgggcgcgc tgcactttac caaaaagcag
     2821 ccccgccacc tggcgatcgg caaacgcctc gcgcaggcta tcaataagct gttgctgggc
     2881 ctgagactcc ccctgcacca tcgccaacac gcgatcatac tctttcatct gcaacagacc
     2941 attgagcgtc gacatccaat tgagatgctc gtgtcgcaat gtgcgcaggc tctcgacgta
     3001 ttgtttaatt tgcgtcagtt gcgcattgag ggtggatatt tcgtctttac tacgaaagct
     3061 gataatggcc cccagcaaat catcaccaga acgaatagct tcccggttgg caataacgct
     3121 cagaccgtta aagttcgcca ccacatcctg acgtttttca tcaatctgtt cggtaaagaa
     3181 atcggcgggc ctgaccactt cagcaatggg tttacccaac cattgccgtc cgggggaact
     3241 cagacccagc atctttcttg cgttacgatt gatggcggta atgtaaccat gcggatccac
     3301 cgcaatgagc ccttcataaa ccgaactaaa cagcgcctct tgctgacgga ccacgcgtgc
     3361 gatttgcttt ggctccatgc ccatcatctg ccgacggata tgcgcggcca ggaaccacga
     3421 tagcaacatc agaatcccta acagcacgac aaacacacct gccatcggta ataaaaactc
     3481 agcacgccag ctatcgattt tactcaccag gtagccaatc gacaccacgc cgatgacttt
     3541 tccatcgtca tcaaagattg gcgttttggc acgcatcgcc atgccaattg accctttccc
     3601 ggtgatgaag tagctctccc ctttctccag cgcgcccggt ttggtgaact gcataggata
     3661 accaattttc tccggattag gatggtaaag acgaatcgag tgccgatcgc caatcaccac
     3721 ataatcgaaa tcagtatctc tttgtaattt gtcagcgatg gtcgccagcc tcttgtagtc
     3781 acgcgttttc actgcgctga tgatactgtc attagaagca ataatttttg cctgattcat
     3841 tgccatgtcg cgcacatgaa gcgttaaata gtcctcaaaa ctggccgtaa aatattgcgc
     3901 cagggctgca atgacaaata ttgagaacac cagaatcagc aaaaagatac gcagcggaaa
     3961 tgccagtctt tggaaaaatg caaactgttt attctcgtta agctgcaaca ttgttttcct
     4021 tgcgggttgt cccgagcctc tttgacagat gtaattaaaa tgttgaatca aaatacatag
     4081 atattaaaca taatcgtggc aaattttatt cattaaatca attaaatcac ttaaacctgc
     4141 gggtcatttc gtaattacat taattaaaaa acttaaaacc attaaataaa taaaaaacca
     4201 ctaactctat gtgaaataaa tcaaaatttc acgctgcaat actccttagg atgtatagcg
     4261 aaaggagaaa aagatatacc tcgatcgccc cctttctccc aagtgaaaat aaaaggttat
     4321 cagtttgcaa catcgaacaa catttgttgc aaatcgataa caacatgcac cttcaggata
     4381 ctatttatta tgttcggcaa tgatattttc acccgtgtaa aacgttcaga aaataaaaaa
     4441 atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac cacagtcgaa
     4501 gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat
     4561 attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta
     4621 gccaccgagc taattaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc
     4681 aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgctgac cagtgtaccc
     4741 ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga aacgctatgc cgaatcgctg
     4801 aaaaaatttc gtcatccagg gaacaagatt ggctgcattg tgatgaacgc caatcccttt
     4861 acgaatggtc accgttatct gattcaacag gctgcagcac agtgcgactg gttacatctg
     4921 tttttagtta aagaagattc ttcacgcttt ccctatgaag accggctgga tctggtgtta
     4981 aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata catcatctcc
     5041 cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc
     5101 gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg cgctgggtgt aactcaccgc
     5161 tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac
     5221 tggctggaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat tgagcggctg
     5281 cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat
     5341 ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt
     5401 gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa
     5461 aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac
     5521 gcatcgcccc actcgatacg caggatatcg acctgcaaat caacagcagc gttgagaaac
     5581 agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg
     5641 gcgtacagct gaatgtcgat gacaaaggcg cactggactg cattttacgt gcacgactgg
     5701 aagccctgct ggcacgcgcc agcggtatcc cggctctgcc atgggaggat tgccaatgat
     5761 ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg
     5821 tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga
     5881 cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg tttaccacgc
     5941 gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtaaacg cgctggattc
     6001 cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct
     6061 gccgaaaacc gataccgctc aggatgttct ggatatcgaa aaagagatcc tgcgtatcga
     6121 aaaagcctgt ggtcgtgaac ccggcagtac cggcttgctg gcggcgattg aatctccgct
     6181 gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct
     6241 cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact
     6301 gctgttcgct cgctgctcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga
     6361 taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa
     6421 acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa
     6481 cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga
     6541 agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc
     6601 ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga
     6661 ataaggcaat caaaatgacg cagaaaattg aacaatctca acgacaagaa cgggtagcgg
     6721 cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcgcca aagcaaacct
     6781 accaggctga aaaagcgcgc gatcgcaaac tgtgcgccaa cctggaagaa gcgattcgtc
     6841 gctctggttt acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc
     6901 tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgactctag
     6961 cctccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg
     7021 ttacccgcat ttatacctct ggcctgcgcg gtccactggc ggaagaaatc tcccgcggtc
     7081 tgctggcgga accggtgcaa atccactctc acggcggacg tgtgcatctg gtacagagcg
     7141 gcgaactgaa tattgatgtg gctttcctcg gcgtcccatc ctgtgatgaa ttcggtaatg
     7201 ccaacggcta caccggtaaa gcctgctgcg gctccctcgg ctatgcaatg gttgatgccg
     7261 acaacgcgaa acaggttgtg atgctcactg aagaactgct gccttatccg cataatccgg
     7321 caagcattga gcaagatcag gttgatctga tcgtcaaagt tgatcgcgtt ggcgatgccg
     7381 cgaaaatcgg cgctggcgcg acccgtatga ccactaaccc gcgcgaactg cttattgccc
     7441 gtagcgctgc ggatgtgatt gtcaactcag gctactttaa agaaggtttc tccatgcaga
     7501 ccggcaccgg cggcgcatcg ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc
     7561 gcgatattcg cgccgacttc gcccttggcg gtattaccgc aacgatggtt gatctgcacg
     7621 aaaaagggct gatccgcaaa ctgctggatg tacagagctt tgacagccac gctgcacaat
     7681 cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt
     7741 caaaaggcgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca
     7801 cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt gcttccggtg
     7861 gtcactgcga taccgcgatt gcctctgcgc tttccatcat cgtcgcgccg ctggtacgcg
     7921 gtcgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg
     7981 atattctggt cactgaccac ggtatcgcag ttaacccggc acgtccggag ctggcagaac
     8041 gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgtgaa cgtgcgcgtc
     8101 tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt
     8161 accgcgatgg ctcggtgatc gatgttgtgc atcaggtgaa ggaataagcc atgcacctgc
     8221 ttcctgaact cgccagccac catgcggtat caattcccga gctgctcgtc agccgggatg
     8281 aaaggcaagc acggcaacac gtctggctca agcgccatcc tgttccactg gtctccttta
     8341 ccgtggttgc gcctgggccg attaaagaca gcgaggtcac acgccgaatt tttaatcatg
     8401 gcgtgacagc cttgcgtgcc ttagccgcaa aacagggctg gcaaattcag gagcaggctg
     8461 cactggtttc cgccagcggg ccggagggca tgttgagcat tgccgccccg gctcgcgacc
     8521 tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg
     8581 atgtcctgac acccgaaggc gaaattctct cccgccgcga ctattcactg ccgcctcgcc
     8641 gctgcctgtt gtgcgagcaa agcgcagctg tttgcgcgcg tggaaaaacc catcaactga
     8701 ccgatttact caaccgcatg gaggcgctgc tgaacgatgt cgatgcctgc aacgtcaact
     8761 aaaaacacaa agcttgcgac gtcattaatc gatgagtacg ccctgctggg ctggcgcgcc
     8821 atgctgactg aagtcaatct gtcaccgaaa ccaggcctcg tggatcgcat taactgcggt
     8881 gcgcataaag atatggcgct ggaagatttc caccgcagcg cgctggcgat tcagggctgg
     8941 ttaccccgtt tcattgaatt tggtgcctgt agtgctgaaa tggcaccaga agaggtactc
     9001 cacggattac gcccaatagg tatggcttgc gaaggtgata tgttccgcgc cactgcgggc
     9061 gtaaacacgc ataaaggcag cattttttct ttagggctac tgtgtgcggc aattggccgt
     9121 ttgcttcaac tcaaccagtc gataacgcca ataacaattt gtgcgacggc ggcaagtttc
     9181 tgccgtggcc tgaccgatcg cgaactgcgt accaataatt cacaactgac ggcaggtcaa
     9241 cggttgtacc agcagcttgg ccttaccggc gcacgcggtg aagccgaagc gggttatcca
     9301 ctggtgatca accacgccct gccgcattac ctcactctgc tggatcaggg gttagatcct
     9361 gaactggcac tgctcgatac cttactccta ctgatggcga tcaacggcga taccaacgtt
     9421 gcatcgcgcg gtggcgaggg gggcctgcgc tggctacagc gcgaggcgca aacattattg
     9481 caaaaagggg gcatccgaac ccccgccgat ctcgattatc tccggcagtt cgacagggag
     9541 tgtatcgaac gaaatctcag tccaggcggc agtgccgacc tgctgatcct tacttggttt
     9601 ttagcacaga tttaattatt taagcacttg ataaaattgg aatattaatt gtcggagaac
     9661 ccgtatgtct ttagcaaaag ataatatatg gaaattattg gcgcccctgg tggtgatggg
     9721 tgtcatgttt cttatccctg tacctgacgg tatgccgccg caggcgtggc attactttgc
     9781 agtgtttgtg gcaatgattg tcggcatgat cctcgagcca attccggcaa cggcgatcag
     9841 ttttattgcg gttactattt gcgttattgg cagtaattac ctgctctttg atgccaaaga
     9901 attagctgac ccagcgttta atgcgcaaaa acaggcgctg aaatggggtc tggctggttt
     9961 ttccagcacc acggtatggc tggtatttgg cgcatttatt tttgcattag ggtatgaagt
    10021 ttccgggtta ggtcgtcgca ttgccctttt cctggtgaaa ttcatgggca aacgcacgct
    10081 gacgttgggt tatgcgattg tcattatcga cattctgctg gcaccgttta caccgtccaa
    10141 caccgcgcgt accgggggta cggtttttcc ggtcattaaa aacctgccgc cgctgtttaa
    10201 atcattcccg aacgatccgt ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt
    10261 cattagtacc agtctgagtt cgtccatgtt tgtcaccggt gcggcaccaa acgtactggg
    10321 tctggagttc gtcagcaaaa ttgccggtat ccagattagc tggttgcagt ggttcctctg
    10381 cttcctgccg gttggggtta tcttgcttat cattgcgccg tggctttcct acgtgctgta
    10441 caaaccggaa atcacacaca gtgaagaagt ggcaacctgg gcgggtgatg aactaaaaac
    10501 catgggtgcg ctgacacgca gagagtggac gctgattggt cttgtattgc tcagcttagg
    10561 tttgtgggta tttggcagtg aagtcattaa tgctactgcg gtaggtctgc tggcagtttc
    10621 gctaatgctg gccctgcacg ttgtgccgtg gaaagacatt acccgctata acagcgcatg
    10681 gaacacactg gtcaacctgg caactctggt tgtgatggct aatggcctga ctcgttctgg
    10741 ttttattgac tggttcgccg gtaccatgag tacgcacctg gaaggattct caccaaacgc
    10801 aacggtgatt gtactggttc tggtgttcta ctttgcgcac tacctgtttg ccagcctgtc
    10861 tgcgcacacc gcaaccatgc tgccggttat tctggccgtc ggtaaaggta ttccgggcgt
    10921 accaatggaa caactgtgta tcctgctggt gctgtctatc ggtatcatgg gctgtctgac
    10981 gccgtatgca accggtcctg gggtgattat ttacggctgt ggctatgtga aatcaaaaga
    11041 ttactggcgt cttggcgcaa tcttcggggt gatttacatc tctatgttgc tgttggttgg
    11101 ctggccgatt ctcgccatgt ggaactaata cgttcgccaa acgctgaata aa
//
LOCUS       DAOTRK010000147        11119 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.148, whole genome shotgun sequence.
ACCESSION   DAOTRK010000147 DAOTRK010000000
VERSION     DAOTRK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.148"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            509..2140
                     /gene="oppA"
                     /locus_tag="QIE41_003863"
     CDS             509..2140
                     /gene="oppA"
                     /locus_tag="QIE41_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDS3532695.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2226..3146
                     /gene="oppB"
                     /locus_tag="QIE41_003864"
     CDS             2226..3146
                     /gene="oppB"
                     /locus_tag="QIE41_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDS3532696.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3161..4069
                     /gene="oppC"
                     /locus_tag="QIE41_003865"
     CDS             3161..4069
                     /gene="oppC"
                     /locus_tag="QIE41_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDS3532697.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4081..5094
                     /gene="oppD"
                     /locus_tag="QIE41_003866"
     CDS             4081..5094
                     /gene="oppD"
                     /locus_tag="QIE41_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDS3532698.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5091..6095
                     /gene="oppF"
                     /locus_tag="QIE41_003867"
     CDS             5091..6095
                     /gene="oppF"
                     /locus_tag="QIE41_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDS3532699.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGNYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTFQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6148..6477)
                     /locus_tag="QIE41_003868"
     CDS             complement(6148..6477)
                     /locus_tag="QIE41_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDS3532700.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6512..7972)
                     /gene="cls"
                     /locus_tag="QIE41_003869"
     CDS             complement(6512..7972)
                     /gene="cls"
                     /locus_tag="QIE41_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDS3532701.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8115..8288
                     /locus_tag="QIE41_003870"
     CDS             8115..8288
                     /locus_tag="QIE41_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDS3532702.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8343..9596)
                     /gene="kch"
                     /locus_tag="QIE41_003871"
     CDS             complement(8343..9596)
                     /gene="kch"
                     /locus_tag="QIE41_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDS3532703.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9897..10193)
                     /locus_tag="QIE41_003872"
     CDS             complement(9897..10193)
                     /locus_tag="QIE41_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDS3532704.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10417..>11119
                     /gene="tonB"
                     /locus_tag="QIE41_003873"
     CDS             10417..>11119
                     /gene="tonB"
                     /locus_tag="QIE41_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDS3532705.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKING"
BASE COUNT         2914 a         2624 c         2774 g         2807 t
ORIGIN      
        1 gcctttggta gaggaaagtg ctaaataata atcaattgtt aaattattgt gcatttcact
       61 actggaactg taatcagaaa agagagacat gcttaaccaa tctctatttg attgaattaa
      121 aagatgtttg ttaagacatg gatgcaagct atagattctg atacggtcaa taaaagagaa
      181 ttgcttaaca attttgcaaa atgtattggc gagtaagaac cgcatttggt actttccggg
      241 caaccgccag acgattcttt attggtaatg agaataatta acaattaaag agcgtcgcga
      301 aagaataatg tgtctcgaca ggggagacac agtacgaatc gacataaggt gatcgtctga
      361 atcaccagaa taaataaagt cggtgatagt aatacgtaac gataaagtaa cctgacagca
      421 gaaagtctcc gagcctgtgc agggtcccaa tccgggatta cacatgctgg ttaataccag
      481 taattataat gagggagtcc aaaaaacaat gaccaacatc accaagagaa gtttagtagc
      541 agctggcgtt ctggctgcgc ttatggcagg gaatgtcgcg ctggcagctg atgtacccgc
      601 aggcgtcaca ctggcggaaa aacaaacgct ggtacgtaac aatggttcag aagttcagtc
      661 attagatccg cacaaaattg aaggtgttcc ggagtctaat atcagccgag acctgtttga
      721 aggcttactg gtcagcgatc ttgacggcca tccggcacct ggcgtcgctg aatcctggga
      781 taataaagac gcgaaagtct ggaccttcca tttgcgtaaa gatgcgaaat ggtctgatgg
      841 cacgccagtc acagcccaag actttgtgta tagctggcaa cgttctgttg atccgaacac
      901 cgcttctccg tatgccagtt atctgcaata tgggcatatc gccggtattg atgaaattct
      961 tgaagggaaa aaaccgatta ccgatctcgg cgtgaaagct attgatgatc acacattaga
     1021 agtcacctta agtgaacccg ttccgtactt ctataaatta cttgttcacc cgtcaacttc
     1081 accggtgcca aaagccgcta tcgagaaatt cggcgaaaaa tggacccagc ctggtaatat
     1141 cgtcaccaac ggtgcctata ccttaaaaga ttgggtcgta aacgaacgaa tcgttcttga
     1201 acgcagcccg acctactgga acaacgcgaa aaccgttatt aaccaggtaa cctatttgcc
     1261 tattgcttct gaagttaccg atgtcaatcg ctaccgtagt ggtgaaatcg acatgactta
     1321 taacaacatg ccgatcgaat tgttccagaa gctgaaaaaa gagatcccgg acgaagttca
     1381 cgttgatcca tacctgtgca cttattatta cgaaattaac aaccagaaac cgccatttaa
     1441 cgatgtacgt gtgcgtaccg cactgaaact gggtatggac cgcgatatca ttgttaataa
     1501 agtgaaagcg cagggcgaca tgcccgccta tggttacact ccaccgtata ctgatggcgc
     1561 aaaattgact cagccggaat ggtttggctg gagccaggaa aaacgtaacg aagaagcaaa
     1621 aaaactgctg gctgaagcgg gttataccgc agacaaaccg ttgaccatca acctgttgta
     1681 taacacctcc gatctgcata aaaagctggc gattgcagcc tcttcattgt ggaagaaaaa
     1741 cattggtgta aacgtcaaac tggttaacca ggagtggaaa acgttcctcg acacccgtca
     1801 ccagggcact tttgatgtgg cccgtgcagg ctggtgtgca gactacaacg aaccaacttc
     1861 cttcctgaac accatgctgt cgaacagctc gatgaatacc gcgcattata agagcccggc
     1921 ctttgacagc attatggcgg aaacgctgaa agcgactgac gaggcgcagc gcacagctct
     1981 gtacactaaa gcagaacaac agctggataa ggattcggcc attgttcctg tttattacta
     2041 cgtgaatgcg cgtctggtga aaccgtgggt tggtggctat accggcaaag atccgctgga
     2101 taatacctac acccggaata tgtacattgt gaagcactaa tggcaatacg tggggcaggc
     2161 gtgtcctgct ccacggtgtc tgatttttat cgcattacag aaggcacagg ccagaaggta
     2221 gggcaatgtt aaaatttatt ctacgtcgct gtctggaagc gattccgacg ctatttattc
     2281 ttattactat ttcgttcttc atgatgcgcc tcgcgccggg aagccctttt accggcgaac
     2341 gtactttacc gccagaagtg atggccaata tcgaagcgaa atatcatctt aatgatccga
     2401 tcatgacaca gtatttcagc tacctgaaac aactggcgca tggcgatttc ggtccatcgt
     2461 ttaaatataa agattattcg gtcaacgacc tggttgcatc cagttttccc gtttctgcca
     2521 aactgggagc cgcagcattt ttccttgcgg taatactggg tgttagtgct ggcgttattg
     2581 ccgcattaaa acaaaacacc aaatgggact ataccgtgat ggggctggca atgaccgggg
     2641 ttgttatccc tagttttgtg gttgcgccat tattagtcat gatatttgcg atcattttgc
     2701 attggctgcc gggcggtggc tggaatggtg gggcgcttaa attcatgata ttgccgatgg
     2761 tggcgttgtc actcgcctat atcgccagta ttgcgcgtat tacccgtggc tctatgattg
     2821 aggttttaca ctccaacttt attcgtactg cccgggcgaa agggttacct atgcggcgga
     2881 tcattttacg ccacgcatta aaacctgctc tgttacccgt gctctcctat atgggccccg
     2941 catttgtcgg cattattacc ggttctatgg ttatcgaaac catttatggt ttgccgggga
     3001 ttgggcaact gttcgttaat ggcgcattga accgtgacta ttccttagtg ttaagcctga
     3061 ctatcctggt tggtgcgtta accattttgt ttaatgccat tgtcgatgtg ctatatgcgg
     3121 ttatcgaccc gaaaatccgt tactgatact ggagctcgcg atgatgttaa gtaagaaaaa
     3181 cagcgagacg ctggaaaatt tcagtgaaaa gctggaggtc gaagggcgca gcttgtggca
     3241 ggacgcacgt cgacgtttta tgcataaccg tgcggcggtt gccagtctga tagtgctggt
     3301 gctgatcgcg ttatttgtaa tcctggcacc gatgctttcg cagtttgcct atgacgatac
     3361 tgactgggcg atgatgtcca gcgccccgga tatggagtcc ggtcactact ttggtactga
     3421 ctcatccggt cgcgacctgc ttgtacgcgt tgcgattggc gggcgtatct cactcatggt
     3481 cggtgttgct gcggcactgg tggctgtggt cgtcgggaca ctttatggtt cgctttccgg
     3541 ttatctgggc ggtaaagtgg attcggtaat gatgcgtctg ctggaaatcc tcaactcctt
     3601 cccattcatg ttcttcgtca ttttgctggt gacctttttc ggtcaaaaca tcctgctgat
     3661 tttcgtggcg ataggcatgg tttcctggct ggatatggct cgtattgtgc gtgggcaaac
     3721 cctgagtctg aagcgcaaag agtttattga ggcggcacaa gttggcggtg tatcgacgtc
     3781 gggcattgtt attcgccaca ttgtgccgaa tgtactcggt gtggtggtgg tctacgcatc
     3841 acttctggtg cccagcatga tcctctttga atctttcctt agcttcctgg ggttgggtac
     3901 gcaagagccg ttaagcagct ggggtgcatt gctgagtgat ggcgcgaact cgatggaagt
     3961 ctctccatgg ttactgttgt tcccagcggg attcctcgtg gtgacactgt tttgtttcaa
     4021 ctttatcggc gatggcttgc gtgatgccct cgacccgaaa gatcgttaag gagtgcagcc
     4081 atgagcgtaa ttgaaactgc aactgtgccg ctcgcacaac aacaggctga cgcactgctg
     4141 aacgtgaaag atttgcgtgt cacctttagt accccggacg gcgacgtcac ggcggtcaat
     4201 gatttgaatt tttccctacg tgccggagaa acgctgggca ttgtaggtga gtctggttcg
     4261 ggtaaatcgc aaactgcatt tgcgttgatg ggcctgttgg ctgccaacgg acgtattggc
     4321 ggatcggcaa ccttcaatgg gcgtgaaatc ctcaatttgc cagagcatga actcaataaa
     4381 ctgcgcgctg aacaaatctc aatgattttt caggacccaa tgacttcgtt gaatccctat
     4441 atgcgtgtcg gtgagcagtt gatggaagtg ctgatgctgc ataagaacat gagcaaagct
     4501 gaggcgtttg aagagtcggt gcggatgctc gatgcggtaa aaatgccgga agcgcgtaaa
     4561 cgcatgaaaa tgtacccgca cgaattttct ggcggcatgc gtcagcgagt catgattgcg
     4621 atggcattgc tatgtcgacc taagctgctg attgcggatg aacccactac tgcgctggac
     4681 gtcaccgtac aggcacagat catgacgcta ttaaatgaac tgaagcggga atttaatacc
     4741 gccatcatta tgatcaccca cgatcttggc gtggtggcgg ggatctgcga caaagtgctg
     4801 gtaatgtacg ccgggcgcac gatggaatat ggcaatgcgc gcgatgtctt ttatcaaccc
     4861 gttcatcctt attctatcgg tttgctcaac gcggtgccgc gtcttgatgc ggaaggtgaa
     4921 acaatgttga ccatcccggg taatccgcca aacttgctgc gattaccgaa aggttgcccg
     4981 ttccagccac gttgtccgca tgcgatggaa atttgtagta gcgcaccgcc gctggaagag
     5041 tttacgcctg gccgtctgcg tgcttgcttt aaaccggtgg aggaactgtt atgaatgctg
     5101 taactgaagg aagaaaagtc ctccttgaaa tcgccgatct gaaagtgcac tttgaaatca
     5161 aagatggcaa acagtggttc tggcaaccgc cgaaaacgct caaagccgtc gatggtgtca
     5221 ctcttcgcct gtatgaaggg gaaactttag gtgtggtagg ggaatcggga tgcggtaagt
     5281 ccacctttgc tcgcgccatc atcggtttgg tcaaggcgac cgacggtcat gttgcctggt
     5341 taggtaaaga gttgctgggc atgaagcccg atgaatggcg tgccgttcgc agtgatattc
     5401 agatgatttt ccaggatccg ttggcatcgc ttaacccgcg tatgaccatc ggcgagatca
     5461 tcgctgaacc actgcgtact tatcatccga aaatgtcacg ccaggaagtt cgcgagcgcg
     5521 tgaaggcgat gatgctgaaa gtcgggttat tgcctaacct gattaaccgc tatccgcatg
     5581 agttctctgg tgggcagtgc cagcgtatcg ggattgctcg tgctcttatt cttgaaccga
     5641 agctgattat ctgtgatgag ccggtgtcgg cgctggacgt gtcaattcag gcgcaggtgg
     5701 tcaacctgct ccagcagctg caacgcgaga tgggattgtc attaattttt atcgctcacg
     5761 acctggccgt ggtaaaacac atttccgatc gtgtgctggt gatgtatctc ggccatgcgg
     5821 tagaactggg gaactatgat gaggtctacc acaatccact acatccttac acccgggcat
     5881 tgatgtcggc agtccccata cctgatccgg atctggagaa gaacaaaacc ttccagttac
     5941 tggaagggga attaccgtcg ccgatcaacc cgccttccgg ttgtgttttc cgtacccgtt
     6001 gcccgattgc cggtccggag tgcgccaaaa cacgtcctgt tctggagggg agtttcagac
     6061 acgccgtttc ttgcctgaaa gtcgatccgc tttaaacaca ataagggctg acagttgtca
     6121 gccctttttc acgccgaaag cgattattta ttcccgccag atgatatggc acagtttgtg
     6181 atctttttcg cggcataaca gaatgcgggc gaaaacatcg ttgatttcac catcttcact
     6241 gtccgccaga ccaatcacca cttcggcaaa aaagtccggg ttcaaatcga aatcaacatg
     6301 ttcctgccag tcttctgccg gatcgaataa ctccgcgccg ccgcgctctt caaactgaag
     6361 attgaacagc agaacatcag ctggatcgag attgtcggca gccagttcga gaaaaatatc
     6421 gtaggcctgc tcaagcgttt catcttcagt caggcgattg tttagatcca tatccatagt
     6481 cactacctgt ttaacctctg ttggcgacgt tttacagcaa cgggctgaag aagtaaaaca
     6541 gtcgctcggc gacacgttgc cataatggac gttttagcca taaacgggca tcgagcagac
     6601 gagaacgcga aatataatcg tcctgaacgg cggcgaggtc agcaccaaac cctttatcgt
     6661 cgattgccag ggtaatctcg aaattcagcc acagactacg catatcaagg ttaacggtac
     6721 caaccagact taattcgcca tcgaccagca cgctcttggt atgcaataac ccgccttcaa
     6781 actgataaat tttaacccca gcagccagca gttccgtaaa gaacgcgcga ctggcccagc
     6841 caaccagcat tgaatcattt tttcgcggaa ggataatact gacatccacc ccgcgctgcg
     6901 ccgccgtgca aatcgcatga agtaaatcat cgctgggcac aaagtagggc gtcgtcatga
     6961 tcaaatattc acgtgccgaa taagccgcag tcagtaatgc ctggtgaatg agatcttccg
     7021 gaaagccggg gccagaagca attgtgtgaa tggtgtgacc gctggcctgc tcaaacggca
     7081 taatattgac atctggtggt ggcggcagaa tacgtttccc ggtttcaatt tcccagtcgc
     7141 aggaataaat aatccccatc gcggtagcga tgggaccttc catacgcgcc atcagatcaa
     7201 tccattgccc tacgcccgca tcttgtttga agtagcgagg gtcgaccata ttcatgctgc
     7261 cggtgtacgc gatgtaatta tcgatcatga tcatcttgcg atgttggcgc aggtccatac
     7321 ggcgtaaaaa cacacgcatc agattgacct ttaaggcttc gaccacttca ataccggcat
     7381 tacgcattag ctcgggccag gggctgcgga aaaaagccac actcccggcg gagtcgagca
     7441 tcaatcggca gtgaatgccg cgtcgcgcag cggccattaa tgattcagcc acctggtccg
     7501 ccatgccgcc gggctgccag atataaaaca ccatctcaat attatggcgc gcgagctgga
     7561 tgtcgcggat taacgcctgc atcacatcat ctgactcggt catcagttgt agctgattcc
     7621 ctttgacccc tgcgatcccc tgacgacgct cgcaaagctt gaataatggc gcagcgacac
     7681 tgctattttc ttcggcgaag atatgcttac aggctttaag gtcgttaagc cattttgcgg
     7741 tggaaggcca catcgctctg gcgcgctcag cgcggcgttt gcctaaatgg agctcgccaa
     7801 cggcaagata ggcaataatt ccgactaacg gcagaatgta aataatcaac agccaggcca
     7861 tcgcggaggg aactgcgcgt cgtttcatta gaatgcgtaa agttacgcct gcaatgagca
     7921 accagtatcc cagaatggcc aaccaactca ccaacgtata aacggttgtc atagattaga
     7981 aatccttttg aaagcgcatt gttatgagtt tacgcatatg gattcatctg gcaaataaaa
     8041 acgcgggaaa agcactggtc agcgtcgggg gcgggggtat aatgaccatt ctgttattgc
     8101 atagagtagt taacatgaag cggagtagaa cggaagtggg gcgctggcgc atgcagcgtc
     8161 aggctagccg acgtaaatcg cgttggcttg aggggcaatc gcgccgaaat atgcgtatcc
     8221 acagcatcag gaagtgcatt ctaaacaaac agcgtaactc gttattgttt gcgatctaca
     8281 atatctaaat gtactgggca ccattgcggt gcccaataag tttactcttc cgtctttaaa
     8341 ctctattttt gcgccgattc tttactgtca gctttcgttt cttgttcatc gttatcgctg
     8401 aaaatgccat gaccggagtt caacaacatt gaaacgagca tatcattatt aatctcttca
     8461 ccatttaaaa ctcgcgccag aatttcgctg ccaaacagtt gcggcgagag aatgatatcc
     8521 ggatgtacca tcttaatctt attcaggttt ttgctatcac tgacggcgag aactgttttg
     8581 acatcactgc tcatatcttt tgccgagagt acaacaaacg cgttatctgc atcgttatca
     8641 ctcagcgcca gaatggcccg gcaacgatcg attcccgctt tcttcaagac tgaactgtca
     8701 ttactgtcac cggggataac atcagcgtta tcgcctaagc gttgctcaag ttgcttgata
     8761 tcatcttcag gcaagttgct gataaccgtt acgttttgtc cgcgttgatt cagttgcagg
     8821 atcgtattga tggcgagaat cgaatgtccg caaacaataa aatgatcttt acgatgcatt
     8881 gtatgattgt ttccttttac aagtttgttg aatcccccgc ggataagcgg accaaaaatt
     8941 gaggtcatag aggtggcaaa aacggtaatg ccggaaataa tgaccgaaat agtgaacaat
     9001 cgtgctgatt cagaaacagg gacaatatcg ccatagccga cggttgacat ggtttctatc
     9061 gaaaaataga acgcggtcat caaactttct attcgcggat taaaaccttc gcttaaataa
     9121 agcgcaccgt aggtggagta aaacagtaac gtcgtgaaac taataaatgc gaaaattgtc
     9181 ccggctgcgg cgctactgtg ggagaagtct ttgcgcagta tcagcaaaaa caccagcgta
     9241 aaaatgcaaa atccaatact aaatttcagc cagggataaa aatgcagggt gtaaattagc
     9301 gctatcaaca acaaaatgat actgattgcc catgcgagct ttgcccggaa cagtaagcca
     9361 atggagttca ggacaagaaa gacgccgagc ataaagagtg gggcgtttgc cagcaaggag
     9421 agatccaact ctgaaaatgc tttgatatgg aaaatatcta gcaaattgac cgacatacca
     9481 tagattgttt taaaaataag caatccattt aaaaagacgg ccagcgcgag aatgtcgtgc
     9541 cgtagggtca cccataaatt agttgctgtt tgtttgaatg tagcccagtg actcactgat
     9601 acctctgtaa ccacaatttt gtttaattat ctgagcgtgc acattttctg aacatacatg
     9661 cagcgcgtga atgtgttaaa caatagtaga tgactttatc cgcagtaatc aaaggttttg
     9721 agtgagcaac tctgcgtcag gaaagggcat ttttcctgaa tatgcttaca tcatataaag
     9781 aaatacagat aaagttatta tctgcttgtg ggggtgaatg cactgaccga cctataagga
     9841 aaggccaaac aagaacacgg ttgcaaaaac cgtgccctta aatattgaat ctctattcag
     9901 aacactttct taaatggttt cactgaaacg tgttcataga ctcctgccgc tacgtacggg
     9961 tcagcatcgg cccaggcctg agctgcttcc agcgattcaa attcagcaat aacggttgag
    10021 ccagtaaatc ccgcagcccc tggatcgtta ctgtctaccg ctggcattgg accagctgtc
    10081 aacaaacgac cttcatcatg cagtaactgt aaacgtgcta aatgtgccgg acgaacggaa
    10141 aggcgttttt cgaggctatc agctttgtct tgagcgtaaa taacatacaa cacgggcaca
    10201 actccttgtt cgggaaagtt gtaaagtacg ttatttgaaa gggcgaagat ctgcaacgga
    10261 aagatgatgt ctttgttaag accatgcata aagtaaggtc aattacgcca aaaatgacat
    10321 tttcactgat cctgatcgtc ttgccttatt gaatatgatt gctatttgca tttaaaatcg
    10381 aggcctggtt tttctactga aatgattatg acttcaatga cccttgattt acctcgccgc
    10441 ttcccctggc cgacgttact ttcggtctgc attcatggtg ctgttgtggc gggtctgctc
    10501 tatacctcgg tacatcaggt tattgaacta cctgcgcctg cgcagccaat ttctgtcacg
    10561 atggttgcgc ctgcggatct cgaaccgcca caagccgttc agccgccacc ggagccggtg
    10621 gtagagccag aaccggaacc tgagccgatc cccgaaccgc caaaagaagc accggtggtc
    10681 attgaaaagc cgaagccgaa acctaagcca aaaccgaagc cggtgaaaaa ggtacaggaa
    10741 cagccaaaac gcgatgtcaa acccgtagag tcgcgtccgg catcaccgtt tgaaaatacg
    10801 gcgcccgcgc gcccgacatc aagtacggcg acggctgcaa ccagcaagcc ggttaccagt
    10861 gtggcttcag gaccacgcgc attaagccgt aatcagccgc agtatccggc acgagcacag
    10921 gcattgcgca ttgaagggca ggttaaagtt aaatttgacg tcacgccgga tggtcgcgtg
    10981 gataacgtac aaatcctctc agccaagcct gcgaatatgt ttgagcgtga ggtgaaaaat
    11041 gcgatgcgca gatggcgtta tgagccgggt aagccaggca gtgggattgt ggtgaatatc
    11101 ctgtttaaaa ttaacggca
//
LOCUS       DAOTRK010000148        11051 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.149, whole genome shotgun sequence.
ACCESSION   DAOTRK010000148 DAOTRK010000000
VERSION     DAOTRK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.149"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..520)
                     /locus_tag="QIE41_003874"
     CDS             complement(<1..520)
                     /locus_tag="QIE41_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS3532706.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     LKKLKALAHPTKK"
     gene            884..1180
                     /locus_tag="QIE41_003875"
                     /pseudo
     CDS             884..1180
                     /locus_tag="QIE41_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            2448..3599
                     /locus_tag="QIE41_003876"
     CDS             2448..3599
                     /locus_tag="QIE41_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532707.1"
                     /translation="MGKELDDICTSCPYIDTVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQIRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            3683..4554
                     /locus_tag="QIE41_003877"
                     /pseudo
     CDS             3683..4554
                     /locus_tag="QIE41_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            4772..7168
                     /locus_tag="QIE41_003878"
     CDS             4772..7168
                     /locus_tag="QIE41_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDS3532708.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            7165..8070
                     /locus_tag="QIE41_003879"
     CDS             7165..8070
                     /locus_tag="QIE41_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDS3532709.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            8067..9137
                     /locus_tag="QIE41_003880"
     CDS             8067..9137
                     /locus_tag="QIE41_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDS3532710.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            9273..9956
                     /locus_tag="QIE41_003881"
     CDS             9273..9956
                     /locus_tag="QIE41_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532711.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            9972..10382
                     /locus_tag="QIE41_003882"
     CDS             9972..10382
                     /locus_tag="QIE41_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532712.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            10603..>11051
                     /locus_tag="QIE41_003883"
     CDS             10603..>11051
                     /locus_tag="QIE41_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDS3532713.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHISRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKV"
BASE COUNT         2956 a         2698 c         2870 g         2527 t
ORIGIN      
        1 actttttcgt gggatgagct aaggctttca gcttttttag atacataagc cgcgtttcaa
       61 gaaatcgaac ttgcctttca agatcctcaa tacgtcggtc ttttgacagc tccaatgctg
      121 atgccgcttt ttctggatca actgatattg caatgtttct tttggtgcca atcttgagcg
      181 cgcgtaaacc agcttctccg cgctcttcat aaaccttcag ccacctggct acagaaccac
      241 taccagcaag cataaagtga gcagcagcct gattaaggga catgtgctgc tcgatcacag
      301 ctttcacgac cttaatacgc aactctggat cagcactaac acctttaggt ttgggaatta
      361 aacctttttc tccatgtttt tcatagaggg caacccatgt cctgacctgg gttcggggga
      421 caccaaaacg tgccgagatg atcctgtaac catcatcagt agtgaagtag tgattcacga
      481 cttcaaggcg cttttcaaaa gggtattttg gctttgacat atcaggggct attccatttc
      541 atcgtccaac aaaatgggtg cagtacattc tctgtggaaa caggggcgcg cattagcttc
      601 tcaaccagag agtgtcacaa agacaaatat tgctgaaaat cgcattcatc gttcaggcgt
      661 gaatgcccgg agacaaaagc cgctgtccac acagtccccc tctgacctga acaaaaagca
      721 tacacacaac aggctcacct gaccccgaaa aaccataact tcagcaatac cagtgacacc
      781 agcacgttac ctcagtgcca catcttcaga ccgcgatcaa tctgaaatgt gactatcggg
      841 gcaatatccc tgctttgtgg tacacgataa ccagaaaaca tcccctgaac cgccagccag
      901 aaagactggc cacaaaaggg gtaagctgaa atcgtttatg gattacatcg acatatgcct
      961 ggcagagaat gtccggaatg acgaggttat ctttgcggag attaaggcaa tggtatacgg
     1021 gccgacgttc catgccgcgt tactacattc agcccggacg taaaatgcgc ccgtcaaaaa
     1081 gaacggttcg cttcgaaact cagccaggat accacctctc cgccacaccc gggaagaagt
     1141 tgatatggtg gttgccggac aacagcgctc ctggattgac tgctgcatca ctcaaccacg
     1201 ctgaatatca aaggagagcg ttaccggtta aaagagaaac gtaaagctgt ggtgatgacc
     1261 caaaacgcaa cgcctgtcag tgaggatgaa atggtgaaaa gcggacagca tcagtaacgg
     1321 aagtatctta gcgggtacga aaatggcagg atcgtgacat tgacagaata tctcctgatc
     1381 atcctgaatg actgcagttg ctgtagaaaa gtaagctgtc tccacccacg gcaccactta
     1441 acaggcattc cgtaacttcc cccagactta ctatgacgca ttatcggtgc actgaaaacg
     1501 ggagagtgcg cttaaaaccg cagaccgcat gcgggtgtta ctgcatgaca gtctggccgt
     1561 attgtgagat gtcggcagga accgatgtcc gcgcaggggg gaacttcctg cagtacctga
     1621 cgcttcaaat ggtattaacc gtagggtgtt gttggttccg gatcgcggaa atgggagcac
     1681 aggtctgcta cggggtgtac ctctgacagt aaatccggct cgctcatctg acagcctctc
     1741 tcccggaaaa gaccaaaaat gagatcgtct cctcaacagc agagaagagg tcttgacaga
     1801 caggctacat tgcatacgat ttatgaagaa aaagacagca aatatggagg ttatagcgga
     1861 gaatacccta cgaaattcct atgaaaaacg attgaaaaaa atatcaaatt cgattcgttt
     1921 ttatatgctt tttgggagcg tggcgatgac gaactgttgc atgtgcagag gagaatgcac
     1981 ccggaagcac cagaatcatc cagaaaggca gaacgattac gcatcaaacc aggagacagc
     2041 aatatcaagg taatatactg atttaaaatg aatttacagc aatacgtgaa aacatttttt
     2101 acttaaagga gacagatgcg ctgaagagag tgcaggtatt tttatataaa aatgagaagt
     2161 ttacgggatg tatatccggc acactatgac cggagaatat cagtcaagct taaaaagaca
     2221 ataaatacag tgagttaaat acacagagaa aacgtagtat cagagggtac atcttacaac
     2281 agagactgaa tttccggttt ttcataaccg gcgcattccg gaaagtgcag gcggcccgga
     2341 ggttttctga cgccactacc tggtagattg ttctgtacat catgtacgga ggtttttgtt
     2401 tgctttttta aaatctgcaa atgtgagaaa agcaggtgag gtgtattgtg ggaaaggaac
     2461 tggatgatat ctgcaccagc tgtccgtata tcgatactgt aaaacggcac aaacaacagc
     2521 tcggtgccat agaggaatat acacagtggt taaaaaaaga accacgggca tcttattttt
     2581 ttctgttcca gctgtatacc cgcattcaca atacattttt ctcccaaaag caacaactcc
     2641 cgttcactcc cggtggcact cactgcccgg aacctgatgt cacccttcgg gacttaacgc
     2701 tttcccctgg ctaccacagt gattatgccc ctcaacccat tccggagatg gacagttctg
     2761 ctgttgttcc cccaactaat gaaaacacct cgcctccgga agatactccc gacaacactc
     2821 cggccggagg caataccgga caagcggaaa agaccagaaa cgcaggactc acacccattc
     2881 ctgaaaaacg ttcaggaatg cccccggaac atctgcggtt tgccacgggg tttccatcgc
     2941 aaccgaagat tgccggtccc agagaaaagc cgatgcgcac cgtgcatccg gataaaatct
     3001 accgtgaaat tatatggttc tgctccagtt acctgctgaa atccggacca gaggctacga
     3061 ggacgattat caactcagta ttctctgaat gggccagcat aaacaatgat tacccctccc
     3121 ccttttcgtg ggtggacagc agggacagtg aacagtgtga ctggttatgg aacgccatgc
     3181 agatccggtg tgtgggaacc ccgctgaatc cccttacccc ggagcagaaa tactggtttg
     3241 cctgcgccac gtttgataac tgggagggct ggaatgagca acaggtacag tttttactgg
     3301 aaagtaatcc cagacggaac agagcgaagt ttacgcaggt ctccttccag gcccccagga
     3361 ttcagcataa agcgattctt cttgatgagc tgaaaagtgc ccgggagcaa caaaaaaggc
     3421 gtgatgaacg tgctgatggt tccgtccctc tgaaactgtc cggaaaaatc cacaaacagc
     3481 ttgaaagtat tgcccgaagt cgtggtgtcc tcccaaaaaa actgctgaat gaaatgattg
     3541 agcaggcgta ccatgatttg gtggcaacca ggcagaacag ccaaatcgat tccagataat
     3601 ttcagacaga tattatttcc gtgaatcccc cgccaccttt ccggtgcgcg gggttttgtc
     3661 ttttttcacc gggaatacat gtatgaatcc gtctgatgcc attgaggcaa ttgaaaaacc
     3721 gctctcctcc ctgccttact cactttcccg tcacatcctg gaacatctgc gcaaactcac
     3781 cagtcacgaa cccgtgcctg gcattatggg taaaagcggg gccggtaaat cctcactctg
     3841 taatgcgctg tttcaggggg agatcacccc ggtcagtgat gttcacgccg gcacccggga
     3901 agtgcagcgc ttccgtctga atggccatgg tcacagcatg gttatcactg acctgcccgg
     3961 ggtgggcgag agccgggaca gggatgcaga gtatgaagcc ctgtaccgtg acattctgcc
     4021 cgaactggac ctggtactgt ggctgattaa agccgatgac cgtgccctgt ctgtggatga
     4081 gtatttctgg cgacacatcc tgcaccgggg acatcagcgg gtactgtttg tggtgatgca
     4141 ggccgataaa aggagccctg ccatgaatgg gatatggccg gtattcagcc ttctcccgca
     4201 caggcacaga acattcgcga aaaaacggag gcggtattcc gtctgttccg gcccgtacat
     4261 cgggttgttg ccgtatcggc ccgcaccggc tgggaactgg atacgctggt cagtgcgctc
     4321 atgacagcac ttcccgacca tgccgccagt cccctgatga cccgcctgca ggacgagctg
     4381 cgcacggaat ctgtccgcgc tcaggcccgt gaacagttta ccggtgcggt ggaccggata
     4441 tttgacacgg cggagagcgc ctgtattgcc tctgttgcac gcacggttct gcgtgccgtc
     4501 cgtgactcgg tggtctctgt tgcccgcgcg gtatggaact ggattttctt ctgaacccgg
     4561 tggtgatgta ccaggctgtt gttacagatt tttaactcca tgaaaacact atgcaaacgc
     4621 agccataccc gggttgtccc cttgcgttgt gtttctcaag ttaatagaat acatcgatcg
     4681 ttactatcat atcgatcgtt ataaacgatc gtttgtagta aatgacctcc tccgtggttg
     4741 tcatgaaaat gcgtcttagg aaggttaaat aatgcacgaa aagaacatcg ccctgctttg
     4801 tgatgaagcc gaccgacttt tgcaactgaa cattaatctg ctccggcaaa tggttgagga
     4861 gccagatgtg ttatctgaca gtaagaacga aaacagactg ctttttgata aacagaaagc
     4921 actgaaaaga attgaggagc tggagggcga acaaatcaaa accgcccgca gggagatggt
     4981 gctggctgtt gtcggcacga tgaaagcagg caaatcaacc accatcaacg ccattgtggg
     5041 gcaggaaatt ctgcctaacc gtaaccgccc catgacctct gtaccgacgc tcatccgcca
     5101 cgttcccgga aaaactgagc cggttctcca tctggaacat attcagcctg tccgcaattt
     5161 attaatcaca ctgcaggaaa aactcgccac cccggcagga cagcaggtcg cacagaccct
     5221 gcagcaaacc ggggataccc gcgaactgct ggatattctg acggatgatg gctggctcaa
     5281 aaatgaatac cacggggagg aggaaatctt taccggactg gcatcgttaa acgatctggt
     5341 tcgtcttgct gcggcaatgg ggactgaatt tccttttgat gaatacgcag aagtgcagaa
     5401 actgccggtg atcgacgtgg aattcagcca tctggtgggg atggatgcat gccagggaac
     5461 actcacactg ctggataccc ccggccctaa tgaggccgga caaccgcaga tggaagtgat
     5521 gatgcgggat caactgcaga aagcctctgc ggttctggct gtgatggatt acacccagat
     5581 gaactcaaaa gcggatgaag acgtccgtaa agagcttaat gccattgctg acgtatcagc
     5641 cggccgcctg tttgtactgg tcaataaatt tgatgagaaa gaccgcaatg gcgatggggc
     5701 agatgccgta cgccagaaag ttccggcaat gctgaacagc gatgtgctgc ccgcctcccg
     5761 cgtttatccc ggatcctcac gccaggcata cctggctaac cgtgcgcttc atgagttacg
     5821 gaaaaacgga acccttcctg ttgatgaagc ctgggtcgat gattttatca gaggtgcctt
     5881 cggttgcatg aaaaaagaat acgtctgtaa agacagtgaa ctggcaactg aaggggcgac
     5941 agacctgtgg gaaggctcac ttatcgatca actgataacg gaagtcatac agagctcaca
     6001 ttccagagct gcggcactgg cggttgactc tgccgccgca aaactgatgc agaatgcaga
     6061 aaatatcagt gaatacctgt tgttacgcca tcaggggcta cagcaaagca ttcagtcact
     6121 gcagtcgcat atcaccagcc tgcttgcgga tatccgggaa atcgcggact gtcaggagca
     6181 gatgaccact gatgtcagaa tggccatgga ggagatcgat actaaaaccc gggaattact
     6241 gacgggggtc tgcacctcac tggaagagga gctgaatgac tatttcagaa gcggtaaacg
     6301 caaagaacag caaatgctgg aggaagaaga cgcagaacaa cgaaggtctc agtccggatt
     6361 atgggggaaa atttctcaat ggtccggtat caacaaccag ggccgggagg attacaggaa
     6421 acgagatttt gccccggaca gccccgaaat aaaattcagt gatcgcaggg aagcccttga
     6481 actgatgacg caaatcgaat cgaccgtgac cagcctgcac cgtgaggctg aagcacagtt
     6541 ccggcctgag ctggagaaaa tcgtcagcgg gattgaaaca ggttttcgtg gcacggccct
     6601 gtacgccaca gaaaacattg ccggtcgcat caatgcccgc ctggaggatg agggctttac
     6661 cgtaaaaatc agttttccgg cagtcagcca gttacagacc cggctcgcgg taaaaataaa
     6721 tctgagtgcg cttatggagg aaagaacgga gaccgtcacc cgtcgccgtc ggcagagtgg
     6781 cgtatgggga accgtttgtc gatggtttgg caccagtgac ctgggctggg aaaactatga
     6841 cgaggatgtg agtcgcagcg tgatcaatat caacaaggtc agagaggaag ttatgtcact
     6901 gacccgggca tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt
     6961 ccgccaggaa atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa
     7021 cacgctgatt cagagctctg aagatcataa acgcgatcag caggcgcagg aacggcttac
     7081 cgggcgactt caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct
     7141 gagggaagag ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac
     7201 cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga
     7261 atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt ccggcgttta
     7321 aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc gtcgcttgca
     7381 cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact tgccaccaca
     7441 aactacgcga ttacccgggt aaacgacagg gtcagttcac tggtcagtga tacagccagg
     7501 ctggcgcatt attccgcaga tacgcgggag cagttactca ccctggccga tcaggttcac
     7561 cataaactga atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag
     7621 ttacatcttg agcagatatt ctcatggtgg agcgccgggc gatatgcgtc attttcccct
     7681 gccggacgtt gttatgtggc acttgaagag cttcgctggg gcgcgtttgg tgatgtgata
     7741 cgtcagagcg aaacaggcca ggttaaccag ctactggata ttctcagaca taaagcatta
     7801 acgcagatgg cacaggagag tggcggtagt gcaaccgtgc gtctgaacac tctggactgg
     7861 cttggtggtc agggccggga gcaggcagat aacgagtggc atgacgcgat taactggctg
     7921 ggagactggt gcagtgaaga gcagcatccg gtgatctggt caaccacgca ggctgcagaa
     7981 cacttaccgg ttcgcatgcc tcgcctctgc tctgcagaac gcctctctga aagtatggtc
     8041 gatgaaatat ttcagaaggg ggctgcatga gtacagaaat gaaaacgggg ctggtgctgt
     8101 ccggtggcgg ggcggtgggc gcttatcagg cgggagtggt taaggcactg gcagagtgtg
     8161 gtacacagat cagcatggtt tcaggggcca gcattggcgc attcaatggt gccattatcg
     8221 cggcctctac cgatctgtca gaagctgccg tacgcctgga ggcgctctgg gatcatctgg
     8281 ggaataatca ggtgctgtcg gtaaacagat tggtttactt ttcattgctg aaaaaattgt
     8341 tccaggcaat gaacctctgc cagatccccg gacgtgcagg agcactgctt acgacgcttc
     8401 ttcgccatat atcgacaatc aacgggtttg acaatctgat ggctcagccg ttgttgtcag
     8461 atgagcccct gacagcgctg atggatcatt atcttgatac tgatgctctg gcagacgggc
     8521 taccgctgta tgtgtcgctg taccccacag aagggggcat gcaggatatt attgactgca
     8581 ttcgtgctga actgggtgtc ggaaccacga aaaacgccgt ttttcagcat atccagagcc
     8641 tgccccgcgg acagcagaaa gaggctctgc ttgcgtcagc cgcgctgccc ctgctgttcc
     8701 gtccccgtga ggttcagggg acaatgttcg gtgatggtgg tatgggagga tggcgaaata
     8761 tgcagggaaa tacccctgtg acgcctctgg tcgatgccgg atgcaatatg gtgattgtga
     8821 cgcatctgag tgacggttct ttatgggatc gccaggcttt tccggacacc acaatccttg
     8881 agatccgtcc ccggaaaagg ctgaaatatg caggtgatgg tggcaacagc ggcggtctgc
     8941 tcagttttac atcggcacat accgacgcct ggcgtcagca gggctatgaa gacacgatgc
     9001 tggcgatgga gcatatccgg aaaccgctgg cagcacgtca ggcactgacc cggtcagagg
     9061 cggtattgca gaaaagcctg gatataacgg aagaggcaga tttggcactg agaaacgcga
     9121 tggcccggat taaataaaga cgccccaaag aaacgccacc gcctgtggtg gtgtttttcc
     9181 tgtctggtgt tattgaaaat attttcagat atatattacc tgagagaaag ttttacccct
     9241 cacctgtcaa ttcactcact ggaaatttaa ttatgaaatt acctgtaaag ttattaatga
     9301 gccttatatc tctggtcagc gttattgcac gtgccgggga atataaaaat tacagccggg
     9361 atgaaataaa atactggcga tatacatcat acaagggggg gaaacttccg gaaggtttca
     9421 ctgatgagaa attttccagc gccatttaca acggaagaat atttacaatg aaacgtttac
     9481 atacccttat gttatttctg gcggttctgt tcacgggctt taactttaac gcagaagcgg
     9541 ctaccgtaaa acaagctctt agttgtaacc cggaagcctg ggctgaacaa cctggagcgt
     9601 gtccatcaac gtacgagttg tacgaaggtg acgctaccta caaagccgct atcgacaaag
     9661 cattaaaacc ggtcggactg agcggcatgt tcggtaaagg cggctatatg gatggccctg
     9721 gtggaggtat cacgccagta aacattaacg gtacagtctg gttccagggc gacggttgca
     9781 aagccaatac ctgcggctgg gactttatcg taacactcta taacccaaaa acccatgaag
     9841 ttgttggcta ccgttacttt ggtttagatg acccggccta cctggtttgg ttcggtgaaa
     9901 ttggcgtgca tgaattcgcg tatctggtga aaaactacgt agctgcggtt aactaattta
     9961 aggagtctat catgaaaact tttatcaaaa ctttactcgt tgctgtaact attctgttct
    10021 ctgtcttcgc tactgcgaaa caagtaaaac tgccaaataa catcaaatac gttaatacta
    10081 cagaagcgtt ttcctgtacc gaaattgacg gtatgaattg ccagacaaag aatcagttca
    10141 actataaaga taacagctat gttttcgtgc ttgagcgtgg tggtgcctgg tgctacgact
    10201 acactgtctc ggtagttaac ctgaaaaccg ggaaagcaca aatgattgaa tacggggaca
    10261 atcaactgtg ctcaggtagc aacaaaccgt tcttcgaaat caaaaatggc gtaccgacgg
    10321 taggagtcat cgacacatcc ggaaaacctg tcgttgtagc ccaggacaaa cttaaaatct
    10381 aaggagtaaa ccatgcgttt ctctgcacat tattaatatc aaaggtaaat acagccccag
    10441 ccattgttac cggaaagccg cttaatgcgt ggctcgccag tgaaaggcct ctcagacaat
    10501 aaaccttatt catttcaccc gtcaggccgc gtcttctccg ggagacgcgg cttttttcat
    10561 ttattgcacc agtaaatctt aaccaccgat aaggagcaaa gtatgcgatt agccagtcgt
    10621 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacatga agaactgata
    10681 cgccatgtac ccagtatttt tggggaagac cggcacatct cccgcagtga acggtatgcg
    10741 tacattccca ccattaccgt cctggaaaat ctgcagcggg aaggctttca gccgttcttc
    10801 gcctgccaga cccgtgtgcg cgacccgggc cgccggggat acacaaaaca catgctgcgt
    10861 ctgcggcggg ccggagagat aaacggagaa catgtccctg aaattattct gctcaactct
    10921 catgacggta cctccagcta ccagatgctg ccgggttact tcaggttcgt ctgccagaac
    10981 gggtgcgtct gtggccagtc tctgggggaa gtgcgtgttc cacaccgggg aaatgtagtg
    11041 gagaaagtta t
//
LOCUS       DAOTRK010000149        10892 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.150, whole genome shotgun sequence.
ACCESSION   DAOTRK010000149 DAOTRK010000000
VERSION     DAOTRK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.150"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            467..694
                     /gene="daaF"
                     /locus_tag="QIE41_003884"
     CDS             467..694
                     /gene="daaF"
                     /locus_tag="QIE41_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1845 fimbrial adhesin operon transcriptional
                     regulator DaaF"
                     /protein_id="HDS3532714.1"
                     /translation="MKINKLTLNERKNDILSYFGEINAPCRTSEVAEHLGVSAYQARH
                     YLQCLEKEGKIKRSPVRRGASTLWEISSIPP"
     gene            complement(957..1898)
                     /locus_tag="QIE41_003885"
                     /pseudo
     CDS             complement(957..1898)
                     /locus_tag="QIE41_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1958..2494)
                     /locus_tag="QIE41_003886"
                     /pseudo
     CDS             complement(1958..2494)
                     /locus_tag="QIE41_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2702..3930
                     /locus_tag="QIE41_003887"
     CDS             join(2702..3017,3017..3930)
                     /locus_tag="QIE41_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDS3532715.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSFVSRCLRVSRAQLHVILRRADDWKDGRRSRHTDDTDV
                     LRRIHHVIGELPTYGYRRVWALLRRQTELDGMPAINAKRIYRIMRQNALLLERKTAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            complement(3942..4688)
                     /gene="istB"
                     /locus_tag="QIE41_003888"
                     /pseudo
     CDS             complement(3942..4688)
                     /gene="istB"
                     /locus_tag="QIE41_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
     gene            complement(4688..5860)
                     /gene="istA"
                     /locus_tag="QIE41_003889"
     CDS             complement(4688..5860)
                     /gene="istA"
                     /locus_tag="QIE41_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HDS3532716.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSRDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            complement(6029..6784)
                     /gene="istB"
                     /locus_tag="QIE41_003890"
     CDS             complement(6029..6784)
                     /gene="istB"
                     /locus_tag="QIE41_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
                     /protein_id="HDS3532717.1"
                     /translation="MSNIHHLERSLRKLRLTRVGAEWHALEKRALAEGWTPSRYLLTL
                     CNEELLWRESEKLRRYKKEARLPVAKTLSEYDFSQVPELNGAQFRQLCETTDWVDAGE
                     NVLLFGASGLGKSHLAAAIVDGVVGQGYRARFYSAGELLQELRKARAQLKLNELLLKL
                     DRYRVIVVDDLGYVKRDSAETGVLFELIAHRYERGSLVITSNHPFSMWGSIFVDETMA
                     VAAADRLIHHGYMFELKGESYRKKTAKAVTSAT"
     gene            complement(6781..8247)
                     /gene="istA"
                     /locus_tag="QIE41_003891"
     CDS             complement(6781..8247)
                     /gene="istA"
                     /locus_tag="QIE41_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDS3532718.1"
                     /translation="MTLNTSQVSYYMTQRKKGITQHISAMKAGISVRSGRRIEKGEWA
                     KNSVRHWRTRKDPLLKERPALTPTTLLEMLQDKYPGQYPNSLRRTMQRRVREWKLQYG
                     AEQEVMFRQRHQPGLRGLSDFTELKGVVVTIAGKLLAHKLYHFRLEWSHWSWMRVVLG
                     GESFSALAEGLQEALGQLGGVPVEHKTDSLRAAWKQQGEDGRRELTERYAALCQHYGM
                     QGVHNNAGRGHENGSVESAHGHLKRRICQALILRGSNDFSTIEEYQAFITQQVMRHNR
                     NNQDLVKEERLHLKPLPLRRSADYDELTVRVSRSSTINVKHVVYSVPSRLVGQLLRVR
                     LWDDRLSCYVGSSEVMSCPRVRPEKGKTRARRIDFRHVIDSLAKKPGAFCHATLRNDI
                     LPDDEWRRLWRRLCNHLEPDMAGRLMVHALKLAAGYDDISVVAKGMEQMLNTPGNVDL
                     HRLMRFLGIKEKALPVVNVKQHNLSSYEQLLRGKGGSQ"
     gene            complement(8453..8986)
                     /locus_tag="QIE41_003892"
                     /pseudo
     CDS             complement(8453..8986)
                     /locus_tag="QIE41_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9895..10305)
                     /locus_tag="QIE41_003893"
     CDS             complement(9895..10305)
                     /locus_tag="QIE41_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532719.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSDQQKSDCVVLNPEKSHPYIADMAMFLSPLPIVLEWHCVMCDKFF
                     PGKKFCEACGTGIYCRAVCTKSVYSYRSDLLLDP"
     gene            complement(10265..10684)
                     /locus_tag="QIE41_003894"
     CDS             complement(10265..10684)
                     /locus_tag="QIE41_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532720.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAYYTCHLCGSALRYHPQH
                     DTERPWFEHTDDGLTEHGHECPYVQPEHREVQLIKRLQQFVPDALPVVRKACWYCRQC
                     RHAYYGERYCTHCQTGRFSEDGEQNERYKNGAGDHAG"
BASE COUNT         2546 a         3008 c         2694 g         2644 t
ORIGIN      
        1 aaaagtaaaa actaaataaa taatgtaata ttaatttgtg aaattaagaa catggatata
       61 aaacactgat ttatattttc agtatgaaca tataagacac agaaagggaa atccggtaca
      121 tcacctgttt tcgtattgta tgaatacccg tggcgcgaca gcggacttta ttactgcaaa
      181 ccgtcatgct tcccggcctc agaggagagt cacatcagca tgagcgacca ggaccacacc
      241 ggtgagtgtt tttaagatga aaggtatggt ggcagtcaca gttgcgggaa ccggcataca
      301 cccgtgacga taaacaactg tgcagaatga caacaggaga aatgaacagt aatgaggggc
      361 atcattcgca gaacgccgga tatcatacat catccccggt cagctggacg catcaccgcc
      421 agaggaacta taatcatccg gaggctgacc atctgttaat aacggcatga aaataaacaa
      481 gttaacactg aatgaacgta agaatgacat tctttcttat ttcggagaaa tcaatgcccc
      541 ctgcagaacc tcagaagttg ctgaacattt aggggtaagt gcttatcagg ccagacatta
      601 cctgcagtgt ctggagaagg aagggaaaat taaacgctca ccggtacgac ggggagccag
      661 tacgctgtgg gaaatcagca gtattccgcc ataacccgtc aggacagatg tcgttgcgga
      721 caaaaagcgg ttccggaaaa cattttacca ctgtgaatga ccatcatccc tgttcagaca
      781 gcggctgttt ccgtgaaaaa aatcaactgg ttgctggcga tataatgata ataccttcac
      841 ggagtgggtg agggacaggg ctcacccgcc cagtgcaata aaatatgtga gaatgacaca
      901 atataaataa ccgcctccgg gtgcgaatgt cccggaagcg ctccggacta atattgtcag
      961 tgtttcagca tattcagctg gtcatttgaa agccccgtca tctgcagcac cgtctgacgg
     1021 tccattccgg cattcagcat attgcaggca gtttccagct tcccttccag cttcccttcc
     1081 agtttaccct gctcaatccc ctgtgccagt cctcgctcaa tgcccttggc aatacctttc
     1141 tgctcaatgc gttcagccag tgtcataatg ttgtcctcgt gctggggcag acgctgcgcc
     1201 aacgtctgca aaaacgtcac cgtcacatgt tcactgccat tcagcagata gtgaaacagt
     1261 acctgtgcca gttcttcatt cagggagtcc tgtatcagct gaaccagcct gtccatctgt
     1321 aacataaggt cacggctgcg gctgtgcttc atcagcagcg tcagggcccc cagacggcgg
     1381 tgatgcataa tttcatcgtc atccatcacc gtcacatcca ccagatggaa cgctctggtg
     1441 tagattttag ccgcaatatc cgggtcagaa aagccatcca gccagttcat gctgtacgga
     1501 tacggactgc gctcaccgtg gtagaacagc accggtatca ccagcggaag taccttatgc
     1561 gcctcaaagt gtcgctacat cgcagctacc gcatacctga gcattctgaa cgccatatac
     1621 gcgttttccg tactctggtg ctccacgagg cagtaaacat agccatcctc accactgaca
     1681 gtcttcatac tgaacagtgc atcacatgca tactgcttca gctcctggtc cacaaacgtg
     1741 cagggctcca gccgcagcgt cgataaatca cacaactgac gatattcctc cggaagatgc
     1801 agcgagacaa aatcccttgc cacctccggc gacgccataa atgccctgaa agcagcatcg
     1861 tgcggcgtgg atgtgttctt ctcttttttc ctgctcaccc tgtctccctt atgaaggtgt
     1921 tatgacatat tctcctgcat tctgtcagga ctgaacgtca cgttttctga gcggcacgac
     1981 ggatcatctc cgcccagtat ccgacagcct cttcactgta tttataaaca tcctttttcc
     2041 ctcccaggat tttcactaat tccggagaca tatcctgcag ataagtctgc aaatcatgac
     2101 ccagatcttc cggtgactgc aacgcataaa ccgcacacca cctttccccc tgcctgattt
     2161 taagtcccgg gttaagaata tactgtccgt ggttcagcct cagaaagagc tttttgccat
     2221 acgggtgttt gctgttaact tcatgctgag aaagctggct gctgatataa ctccttcgtt
     2281 tgcgacgtcc cggcagcagc gcctccggaa gatggaccac acactctgcc agtcgtgccg
     2341 cactgaaggc ttctgaaaaa accagagcac ctcccagatg cgtgtggaac agcgcctgca
     2401 tcaggagcac caggaacatg gccatctgcc ggttgtccag tttgatgagt cgtccgtcca
     2461 cctgcagtga gagactttct ggtttcagca gggttgtcaa cgccacgatg tttgaccgtt
     2521 atttgccatt ttcatgcccg ctaagatact ttcgttactg atgctgtccg cttttcacca
     2581 tttcatcatc actgattggc gttgtgtttt tagactggcc ccctgaatct ccagacaacc
     2641 aatatcactt aaataagtga tagtcttaat actagttttt agactagtca ttggagaaca
     2701 gatgattgat gtcttagggc cggagaaacg cagacggcgt actacacagg aaaagatcgc
     2761 tatcgttcag cagagctttg aaccgggaat gacggtctcc cttgttgccc ggcaacatgg
     2821 tgtggcagcc agccagctat ttctctggcg taagcaatac caggagggaa gtcttactgc
     2881 tgtggctgcc ggagaacagg tcgttcctgc ctctgaactt gctgccgcca tgaagcagat
     2941 taaagaactc cagcgtctgc tcggcaaaaa aacgatggaa aatgaactcc ttaaagaagc
     3001 cgttgaatat gggcgagcaa aaaagtggat agcgcacgcg cccttattgc ccggggatgg
     3061 ggagtaagct tcgtcagccg ttgtctccgg gtgtcgcgtg cgcagttgca cgtcattctc
     3121 agacgagccg atgactggaa ggacggccgc cgcagccgtc acacggatga tacggatgtg
     3181 cttcgccgta tacaccatgt tatcggagag ctgcccacgt atggttatcg tcgggtatgg
     3241 gcgctgcttc gcagacaaac agaacttgat ggtatgcctg cgatcaatgc caaacgtatt
     3301 taccggatca tgcgccagaa tgcgctgttg cttgagcgaa aaaccgctgt accgccatcg
     3361 aaacgggcac atacaggcag agtggctgtg aaagaaagta atcagcgatg gtgctctgac
     3421 gggttcgagt tccgctgtga taacggagaa aaactgcgag tcacgttcgc gctggactgc
     3481 tgtgaccgtg aggcactgca ctgggcggtc actacgggcg gcttcaacag tgaaacagta
     3541 caggacgtca tgctgggagc ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca
     3601 gtagagtggc tgacggataa tggttcatgc taccgggcta atgaaacacg gcagtttgcc
     3661 cggatgttgg ggcttgaacc gaagagcacg gcggtgcgga gtccggagag taacggcata
     3721 gcagagagct tcgtgaaaac gataaagcgt gactacatca gtgtcatgcc caaaccagac
     3781 gggttaacgg cagcaaagaa ccttgcagag gcgttcgagc attataacga atggcatccg
     3841 catagtgcac tgggttatcg ctcgccacgg gaatatctgc ggcagcgggc ctgtaatggg
     3901 ttaagtgata acagatgtct ggaaatatag gggcaaatcc agtttttggt cagcactcca
     3961 gctttacgtt tctcttttaa ccggtaactc tctcctttga tattcagcgt ggttgagtga
     4021 tgtagcaacc gatccagtat cgctgttgcc agcacgtgat ctccgaacat ttctccccag
     4081 tctgcgaacc ctttgtttga cgtcagtatg atgctcgctt tttcatatcg acggttcagt
     4141 agccggaaga acaggctggc ttcctctctg ttcatcggca gatagcctat ttcatccagg
     4201 atcaacaccc gggcataact cagttgctgc agctgacgct ccagccggtt ttcctgtttc
     4261 gctttcatca gtgtcgcgat cagtctgtcc agtggcataa acagtacccg atgtcccgca
     4321 tccaccgctt tcacgccaag agctatggcc agatgagttt ttccgacacc aggaggtccc
     4381 agcaggatca cgttttcgct gcgctccacg aacgccagac cagccagttc ccggacaacc
     4441 ttacggtcga tgcccggctg gaaggtaaag tcgaactgct ccagcgtttt gacccacggc
     4501 agacgagcct gcttcagcct ggactccata ccgcgctgat gcctgccgtt ccattcctgc
     4561 tgtagcgcca tgcacaggaa ctcccggtag ttcagctctt ttttagctgc ctgttccagc
     4621 aggctttcaa cgtgataact cagatgctcc attttcaggc gactcagcag gacttccagc
     4681 tcatgcatca caacagctcc tcataggcac tcagtggtcg atgttccacc tgactgacct
     4741 gctgccagag cggggcgtga tgctccggca ctgtctgcca gccagacgat gccgaacaga
     4801 ggcgatgtga ggccaccagt ttctcattac tgtagatccg caactcgtca tccagcgata
     4861 ttcgtatcga taccggctga ccacacagcg cttcgggaac gctgtaacga ttaccaccaa
     4921 cctcgatata gctgtcccgg gacacatggc ggatgtcgaa gtaactggta tcgaagtccg
     4981 tatccggtaa cggctgcaga tgttcctgct ccagcgcgaa gcgctgttcc ggcgtttctt
     5041 tgaactggcg aagttcccgt ttgtcagcca catcggctat ccattgctcc agttgttgat
     5101 taacatgagt gaagctgtcg aacctgcggt accgaacgaa gaagttctcc ttgaggtatt
     5161 tcaccatccg ctcaacctta ccttttgttc tggccctgcg tggacggcat gcccgtggca
     5221 ggaagttata gtggtcggcc agcaacagga atccggagtt gaacacgact ttcccgttgt
     5281 tattcttcag caccgcagcc ttctggttat caaccagcac cgttttcaca caaccaccga
     5341 agtagcggaa ggcgcgaacc agtgattcgt aggtatgctc agcatcctgt tttggtgcgg
     5401 cgaagacatg gaagcggcgg gagaacccca gcgtattaac cgcaaagtta actttgcacc
     5461 gttgcccggc aacctccacc tcaacttcgc cccagtcgtg ctggagctgg tatccaggct
     5521 gagtttcgaa gcgaactgtt ctttttgacg gacgcatttt acgtttgggc tggatgtagt
     5581 aacgcagcat ggaacgtccg cccgtataac ccattgcctt aatctccgca aagataacct
     5641 cactattcca gacattctct gccaggcgca tgtcgatgta atccataaac ggtttcagct
     5701 taaccatttt gtggcgggtc tttctggctg gcggttcagg gtatttgagg tagcgtctga
     5761 ccgtccgttc agagcaacca atctgagtcg caatatcgac aatgtacgcg ccctgctggc
     5821 gcatttgctt tatcatgtaa aagtcctctc tgctcagcat gttgatgtcc tttctggtgt
     5881 gagaacctta aggaaacaac atgttgggtg gagcggacaa tccaaatggt gaattaccgt
     5941 cttatatcac tggcgctgac agatattaga cgtcaactag ttttgccgac tcgcgccaac
     6001 tatactggcc gcacccttca gtgcgacatc aagtcgcgct tgttactgcc ttcgctgtct
     6061 ttttcctgta gctttcacct ttcagttcga acatatatcc gtgatggatc agccggtctg
     6121 ccgccgccac cgccatagtc tcatccacga agatgctgcc ccacatgctg aacggatggt
     6181 tactggttat caccaggctc ccacgttcat agcgatgcgc tattaactcg aacagtactc
     6241 ccgtttcggc gctgtcgcgt ttgacatagc caagatcatc caccactatc acccggtagc
     6301 gatccagttt cagtagcagc tcattcagtt tcaactgcgc tctggcttta cgtagttcct
     6361 gcaacaactc tcctgcgctg tagaaccggg cccggtagcc ctggcctact acgccatcca
     6421 cgatcgctgc cgccagatgg cttttcccca acccgctggc tccgaacagc agaacgtttt
     6481 ctcctgcatc aacccagtct gtcgtttcac agagttgccg gaactgagct ccattcagtt
     6541 ccggcacctg actgaagtcg tattcgctta gcgttttggc aactggcaac cgggcctcct
     6601 ttttataacg acgcagtttt tcactctcgc gccacaggag ttcttcattg catagcgtca
     6661 gaagatagcg cgatggtgtc cagccttctg ccagtgctcg tttttccaga gcgtgccatt
     6721 cagctccaac tcgtgtcagg cgtagtttac gcaggctgcg ttcaaggtga tggatattgc
     6781 tcactgcgaa cctcccttgc cacgcagtag ttgctcataa ctgctcaggt tatgctgttt
     6841 cacattgact accggcaacg ccttttcctt tatacccagg aagcgcatca gccggtgcag
     6901 atccacgttt cccggggtat tcagcatctg ctccatacct tttgccacga ctgagatatc
     6961 gtcgtatcct gcagccagtt tcagagcatg taccatcagc ctgcctgcca tgtcgggttc
     7021 cagatgatta cataagcgac gccacagcct ccgccattca tcgtctggca ggatgtcatt
     7081 tctcagcgtt gcatggcaga acgcaccggg cttttttgcc agactgtcga tcacatgtcg
     7141 gaagtcgata cgacgggccc gcgtcttccc tttttctggt ctgacacgtg ggcagctcat
     7201 gacctcgctg ctgccaacgt aacagctcag acgatcgtcc cataaccgga cccgtaacag
     7261 ttgacctaca agccgggaag gtacgctgta gacgacgtgc ttcacattga tggtactgct
     7321 gcggctaacc ctcacagtca gctcatcata gtcagcactg cgacgaagcg gcagcggttt
     7381 cagatgaaga cgttcttcct tgaccagatc ctgattgtta cggttgtgcc gcataacctg
     7441 ctgagtgatg aaggcctgat attcttctat ggtgctgaag tcgttactgc cccgcagtat
     7501 cagcgcctga cagatacgcc ttttcagatg tccgtgggca ctttcaaccg agccattttc
     7561 gtggccccga ccggcattat tgtgtacgcc ctgcattccg tagtgctgac agagagcagc
     7621 ataacgctca gtcagctcgc ggcgtccatc ttcgccctgt tgtttccatg ctgccctcag
     7681 gctgtccgtt ttatgttcta ccggcactcc gcccagttgt ccgagggctt cctgcagacc
     7741 ttcagccaga gcagagaagc tctcaccacc cagcacaacc cgcatccagc tccagtggct
     7801 ccattccaga cggaagtgat acaacttatg cgccaacaac ttaccggcga tggtgacaac
     7861 tacacctttc agttcagtaa agtccgacag acctcgcaga ccgggctgat gtcgctggcg
     7921 gaacatgacc tcctgctctg caccatactg tagcttccat tcgcggaccc gccgttgcat
     7981 tgttcttcga aggctgttgg ggtactggcc gggatattta tcctgtagca tctccagcag
     8041 agttgttggt gtcagagccg gcctctcttt caacagagga tctttgcgtg tgcgccagtg
     8101 ccgaacactg ttttttgccc actctccttt ttcgatccga cgaccagaac ggactgagat
     8161 accagccttc atggccgaga tatgctgagt tatacctttc ttacgctgag tcatatagta
     8221 actgacctga gaagtattga gcgtcacgcc atttcctgtt ggatcatgta aatgacactc
     8281 aggttacaaa accggccaga ataatcggcg ccaggcggac tgagttattg tcgtctcata
     8341 ggtagagaca gattttcatg gctgtcagtt cctgtcgtgt aaaaaacgca ctgattctac
     8401 gaaggaacgg ccctcgcaac aaccttgctc ccttcctgaa tgaaattcac gttcagttat
     8461 cgtcaccgat cacacagccc ctgattgttg tggatataac cggtaatatc aacaatatcg
     8521 cagctgtttt taacgcaacg tactggtctg atgtatcatc acgggcaacc tgaaaacacc
     8581 tttccagccg gcattgtccg cctgcgctta tcacccgcca ggtctgttgc agtaaatcca
     8641 ccacttgcga acattcttca tcaactgtga cactggcccg cgaatggcaa atgagtccct
     8701 gactgaataa cagcaaacat cgcatcgttt ctttttctga atcttcccgc aactagcgta
     8761 acaccaccgg cgaggacgga ctggccatgt gattacgctc agtaatacgt tcatgcacct
     8821 gtcggtaaaa ggaagtacag aatgcatgaa tctgttgccc gttgattcct tctgtagaat
     8881 gaagcgtgaa gtgaactgcc agcagacgcg accagtagtc tacggcctgc tgaataaaac
     8941 gccggatgtc tcccggctct gcaagtaagg cttcggttat ctgcacgaaa tgttcctttt
     9001 caaaccgttg cattttgtta agccggtgca gacacacggg aaagcacgcc tgcaccggtg
     9061 taatgccagt tataaacaat gtgaatggaa tgacgaatca gtcaggaagg gatggcaacc
     9121 tgctggctga ccggagaatg tctgcaggaa ggttcacggg aatgtcgcca gcaccaggga
     9181 cagggcccgg ctccacaata ccagcgccgg cattttgcac actgccagcg caccggcatc
     9241 acaacacgta tcatgcaact ccccctcgtg attcctctgt tcgctgctgc atccactgct
     9301 ccacttcgct tctgtacccg cggctgctac gacccggttt tatgggttca ggaaaatggc
     9361 cttcactgat cagcctgtaa aaccctttgt cggatcggtg aagctggtga tgcctggcac
     9421 atcggcaagc agcaatacgc tgttgtggcc tgttattgag gtcagaatat cctctgtgag
     9481 attgatgaag gtataagggg gaactgctga acggtgcttt cagagctggt ctgctacggg
     9541 agaccaggga ttaccggcaa ggtaacgggc ctccggaacg cgcacaccac gacgatcgca
     9601 ggcgaaatca ctgaacaccc gtatcacttt tgagtctctc ttcgcactcc ggtgaacgac
     9661 tgtacggcac cacatcccgg ggggagcggc ttctggtacg gtgctgcttc acccggtgcg
     9721 ctttcgcatc ccggcacact gaatcctccg ggattctggt aagcagaact gcagatatcc
     9781 gctgtcgcgt cgttgcacgg tgtgggtgcg aaacaccgga taacatgcag gctggtgata
     9841 cctctgaacg tcctgagcag cgcggacttt gtcggattac gtggaaacac cacgtcaggg
     9901 gtccagcagc aaatctgacc ggtagctgta aacagatttc gtacataccg ctctgcaata
     9961 aatccccgta ccacaggcct cacagaactt cttcccggga aaaaacttgt cgcacatcac
    10021 acaatgccac tccagcacga tcggtaatgg cgataaaaac atcgccatat ccgcaatgta
    10081 agggtgggac ttttccggat tcagcaccac acagtccgac ttctgttggt cgctcagggc
    10141 atgtaaatcg tgctcaaacc acgccccctg agcatctgtc tgcagaatca accggccatg
    10201 acaggaaagg cagaaacaat gccggatatt tctgctgagc gcagagtcgg cactgataat
    10261 gtgctcaccc ggcgtgatcc ccagccccgt ttttataccg ttcattctgc tccccgtcct
    10321 cactgaagcg cccggtctgg cagtgtgtac aatagcgttc cccataataa gcgtggcgac
    10381 attgtctgca gtaccagcag gctttacgca ccacgggtaa ggcatccggt acgaattgct
    10441 gcagacgttt aatcagctgt acttccctgt gttccggttg tacataaggg cactcatgac
    10501 cgtgttccgt cagcccgtcg tcagtgtgtt caaaccaggg acgttccgtg tcgtgctgcg
    10561 ggtggtatct gagtgcactg ccgcaaaggt ggcaggtgta gtaagcataa ggtgcggtct
    10621 gtgcggtacg ggcaccggtc agacgtccgt tgccatcaag tgcgagaaaa gattttgcgt
    10681 acatagtgtg gttccttacc gccagacgac acgcaggcaa cagccgtccc ggtacgggca
    10741 gcgtggtcag gtgtgaatgg cggttttcag ttcagtctca gaagggaaaa tcagtgtggt
    10801 cgctgtgaac acgtcatcac actgaagtca gataagtcgt ctgtcgggct cagccacgac
    10861 tgcagccaaa gttgcgctca ccggtgccgt aa
//
LOCUS       DAOTRK010000150        10892 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.151, whole genome shotgun sequence.
ACCESSION   DAOTRK010000150 DAOTRK010000000
VERSION     DAOTRK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.151"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            52..813
                     /gene="adiY"
                     /locus_tag="QIE41_003895"
     CDS             52..813
                     /gene="adiY"
                     /locus_tag="QIE41_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDS3532721.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            950..2287
                     /gene="adiC"
                     /locus_tag="QIE41_003896"
     CDS             950..2287
                     /gene="adiC"
                     /locus_tag="QIE41_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDS3532722.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2391..4034
                     /gene="eptA"
                     /locus_tag="QIE41_003897"
     CDS             2391..4034
                     /gene="eptA"
                     /locus_tag="QIE41_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDS3532723.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNETRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4031..4699
                     /gene="basR"
                     /locus_tag="QIE41_003898"
     CDS             4031..4699
                     /gene="basR"
                     /locus_tag="QIE41_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDS3532724.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4700..5800
                     /gene="pmrB"
                     /locus_tag="QIE41_003899"
     CDS             4700..5800
                     /gene="pmrB"
                     /locus_tag="QIE41_003899"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDS3532725.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGGRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5776..5865)
                     /gene="pmrR"
                     /locus_tag="QIE41_003900"
     CDS             complement(5776..5865)
                     /gene="pmrR"
                     /locus_tag="QIE41_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDS3532726.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5977..7479)
                     /gene="proP"
                     /locus_tag="QIE41_003901"
     CDS             complement(5977..7479)
                     /gene="proP"
                     /locus_tag="QIE41_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDS3532727.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7743..8621)
                     /locus_tag="QIE41_003902"
     CDS             complement(7743..8621)
                     /locus_tag="QIE41_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDS3532728.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8618..10846)
                     /gene="crfC"
                     /locus_tag="QIE41_003903"
     CDS             complement(8618..10846)
                     /gene="crfC"
                     /locus_tag="QIE41_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDS3532729.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADFEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQIKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQAVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2715 a         2861 c         2689 g         2627 t
ORIGIN      
        1 cgctttgctt tagcgagaac tggtctttta ttcgcactca ggagtacatg tatgaggatt
       61 tgcagcgacc aaccttgtat tgttttattg actgaaaaag atgtctggat aagggtgaat
      121 gggaaagaac ctattagcct taaagctaac catatggcgt tattaaattg tgaaaataat
      181 attatcgacg tctcctctct taataacact ttggttgctc atattagtca cgacatcatc
      241 aaagattacc tccggtttct gaataaagat ctctcgcaaa taccagtatg gcaacgtagc
      301 gctacgccca tcctcactct gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa
      361 catagcatga tgcccgctga aactgagtca gaaaaggaac gaacacgtgc attattattc
      421 actgtgctat cccgttttct cgacagtaaa aaattccttt cactaatgat gtatatgtta
      481 cgtaattgtg taagtgacag cgtttatcaa attattgaaa gcgatatcca caaagactgg
      541 aatcttagta tggtagccag ttgtttatgt cttagcccaa gtctgttaaa gaaaaagctg
      601 aaaagcgaaa acaccagtta tagccaaata atcaccacct gccgcatgcg ttatgccgta
      661 aatgaattaa tgatggacgg taaaaatatc tctcaggtat cacagtcctg cggctacaac
      721 agtacgtcgt actttatttc tgtctttaaa gacttctacg gtatgacgcc tctgcattat
      781 gttagtcagc acagagaacg cactgtcgcc tgatttttaa ccttaacgaa gagctatatt
      841 aataacggca tcagcgataa cccggtcgat aataattcaa ctatcgaatg caggcgtatg
      901 atatgacgta atttattgtc acgaagctcg ccttcgcagg agtttaatta tgtcttcgga
      961 tgctgatgct cacaaagtgg gcttaatccc cgtcaccctg atggtgtcgg ggaatattat
     1021 ggggtcaggt gtttttctgt tacctgcaaa cctggcctcc actggcggga ttgccattta
     1081 tggatggttg gtgacgatta tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc
     1141 gttcctcgac ccaagtcctg gtggttctta cgcttacgcc cgccgctgct ttgggccgtt
     1201 tctcggttat caaaccaacg tcctctactg gctggcctgc tggatcggca atatcgccat
     1261 ggtggtcatt ggcgtaggtt atttaagtta cttcttcccg attctgaaag atccattggt
     1321 attaaccatc acctgcgtcg tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc
     1381 gaaaatgatc acccgtgtgc aggccgttgc caccgtgctg gcgctgatcc ccatcgtcgg
     1441 gattgccgta tttggctggt tctggttccg tggtgaaacc tatatggcgg catggaacgt
     1501 cagcggcctg ggcaccttcg gcgcgattca aagtaccctt aacgttacgc tgtggtcgtt
     1561 catcggtgtg gaaagtgcct ccgttgccgc aggtgtggtg aaaaacccga aacgcaacgt
     1621 ccctatcgcc accattggtg gggtattgat tgccgccgtt tgctatgtgc tctccaccac
     1681 cgcgattatg gggatgatcc ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga
     1741 tgccgcacgg atggcgttgg gtgacaccgc cggggccatt gtttccttct gcgcagctgc
     1801 gggttgctta ggttcactgg gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc
     1861 tgccgatgac ggactgttcc caccgatttt tgcccgtgta aataaagcgg gtacgccagt
     1921 ggcagggctg attatcgtcg gtattttgat gaccatcttc cagctcagta gcatttcgcc
     1981 aaacgcgact aaagagttcg gtctggtgtc ttccgtgtcg gtcatcttta cactggtgcc
     2041 atatctttac acctgtgcgg cgttactgct gctcggccac ggtcactttg gtaaagcacg
     2101 cccggcatat ctggcagtga ctaccattgc cttcctctac tgcatctggg ccgtggtcgg
     2161 gtccggggcg aaagaggtta tgtggtcatt tgtcaccctg atggtaatca ccgccatgta
     2221 tgcgctgaat tacaaccggc tgcataaaaa cccgtatccc ttagatgcac caataagcaa
     2281 agattaattc cccttaatca agcaaacata aaagccaacc ttaagaactt aaggttggct
     2341 taattttgct ttgcgagcat atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc
     2401 gcctactaaa aagaccctct ttgaatttac tcgcctggct attgttggcc gctttttata
     2461 tctctatctg cctgaatatt gcctttttta aacaggtgtt gcaggcgctg ccgctggact
     2521 cgctgcataa cgtactggtt ttcttgtcga tgccggtcgt cgccttcagc gtgattaata
     2581 ttgtcctgac actaagctct ttcttatggc ttaatcgacc gctggcctgc ctgtttattc
     2641 tggtcggcgc agctgcacaa tatttcataa tgacttacgg catcgtcatc gaccgctcga
     2701 tgatagccaa tattattgat accactccgg cagaaagtta tgcgttgatg acaccgcaaa
     2761 tgttattaac gctgggattc agcggcgtgc ttgctgcgct gattgcctgc tggattaaaa
     2821 tcaaacctgc cacctcgcgc ctgcgcagtg ttcttttccg tggagccaat attctggtgt
     2881 ctgtactgct gattttgctg gtcgccgcac tgttttataa agactacgcc tcattgttcc
     2941 gcaacaacaa agagctggtg aagtccttaa gcccctctaa cagcattgtt gccagctggt
     3001 catggtactc ccatcagcga ctggcaaatc tgccgctggt gcgaattggt gaagatgcgc
     3061 accgcaaccc attaatgcag aacgagacac gtaaaaattt gaccatcctg attgtcggcg
     3121 aaacctcgcg ggcggagaac ttctccctca atggctaccc gcgtgaaact aacccgcggc
     3181 tggcgaaaga taacgtggtc tatttcccta ataccgcatc ttgcggcacg gcaacggcag
     3241 tttcagtacc gtgcatgttc tcggatatgc cgcgagagca ctacaaagaa gagctggcac
     3301 agcaccagga aggcgtgctg gatatcattc agcgagcggg catcaacgtg ctgtggaatg
     3361 acaacgatgg cggctgtaaa ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc
     3421 tgaatctgcc tggtcagtgc atcaacggcg aatgctatga cgaagtgctg ttccacgggc
     3481 tggaagagta catcaataac ctgcagggtg atggcgtgat tgtcttacac accatcggca
     3541 gccacggtcc gacctattac aaccgctatc cgccgcagtt caggaaattt accccaacct
     3601 gcgacactaa cgagatccag acctgtacca aagagcaact ggtgaacact tacgacaaca
     3661 cgctggttta cgtcgactat attgttgata aagcgattaa tctgctgaaa gaacatcagg
     3721 ataaatttac caccagcctg gtttatcttt ctgaccacgg tgagtcgtta ggtgaaaatg
     3781 gcatctatct gcacggtctg ccttatgcca tcgccccgga tagccaaaaa caggtgccaa
     3841 tgctgctgtg gctgtcggag gattatcaaa aacggtatca ggttgaccag aactgcctgc
     3901 aaaaacaggc gcaaacgcaa cactattcac aagataattt attctccacg ctattgggat
     3961 taactggcgt tgagacgaag tattaccagg ctgcggatga tattctgcaa acttgcagga
     4021 gagtgagtga atgaaaattc tgattgttga agacgatacg ctgttattgc agggattgat
     4081 tctggcggca caaaccgaag gctacgcgtg cgatggcgtg acaaccgcgc ggatggcgga
     4141 acaaagcctt gaggccggtc attacagcct ggtggtactg gatttagggt tacccgacga
     4201 agatggactg cattttctcg cccgtatccg gcagaaaaaa tacaccctgc cggtactgat
     4261 cctcaccgct cgcgatacgc tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga
     4321 ctatctggtg aagccttttg cgctggaaga gttacacgcc cgtatccgcg ccctgctacg
     4381 acgccataat aatcagggcg aaagtgagct gattgttggc aatctgacgc tgaatatggg
     4441 tcgccgtcag gtatggatgg gcggtgaaga gttgattctg acgcccaaag aatatgctct
     4501 gctgtcacgg ttaatgctca aagcaggcag tccggtgcat cgggaaattc tctacaacga
     4561 catctataac tgggacaatg aaccctcgac caacaccctg gaagtgcata tccacaatct
     4621 gcgcgacaaa gtgggcaaag cccgtatccg caccgtgcgc ggctttggct atatgctggt
     4681 cgcgaatgag gaaaactaat tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg
     4741 ccaacggctg atattgacca ttggggccat tttgttggtg tttgagctga tcagcgtctt
     4801 ctggctatgg catgaaagta ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa
     4861 tcgcaacaac gatcgccaca tcatgcgtga gatccgcgag gcggtcgcca gcctgatcgt
     4921 ccccggcgtc tttatggtca gcctgacgct atttatctgc tatcaggcgg tacgccgcat
     4981 cacccggccg ctggcggagc tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac
     5041 gcctattgcc attcacagcg ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga
     5101 tctggtcagt cgcctgacca gcacgctgga taacgaaagg ttgtttaccg ctgacgtcgc
     5161 gcacgaactg cgaacgccac tggcgggggg gcgtttgcat ctggaactgc tggcgaaaac
     5221 gcatcacatt gatgtagcac cgttagtggc tcgacttgat cagatgatgg agagtgtctc
     5281 ccagctgctg caactggcgc gtgccggaca gtcattttct tccggtaatt atcaacatgt
     5341 aaaactgctg gaagatgtca ttctcccctc gtatgacgaa ctcagtacca tgctcgacca
     5401 gcgacagcaa accctgctat tgccagagag cgccgccgac atcaccgttc agggtgatgc
     5461 aaccctgctg cggatgttat tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca
     5521 aggcagcaac attatgatta agctgcaaga agatggcgga gcggtcatgg ccgttgaaga
     5581 tgaaggaccg ggtattgatg agagtaaatg cggggagttg agtaaagcgt ttgtacgtat
     5641 ggacagccgt tatggcggga ttggtctggg gttaagtatt gtcagccgca ttacacagtt
     5701 gcatcacggg cagtttttcc tgcaaaaccg gcaagagact tccggcacgc gggcctgggt
     5761 acggctgaag aaagatcagt acgtggcaaa ccagatataa agaaagctgc tgaccaccag
     5821 cacgctgaac acggtagtta aactttcata aacacggttt ttcattgcac tctcctccgt
     5881 taacctggag gagagtatgc gcgtcagaga ttaagtgaac cttaagactt caattggcct
     5941 gataagacag cgtcacatca ggccatccgt ttcagcttat tcatcaattc gcggatgttg
     6001 ctgcaccagg cgagtacgtt tcgcctgcaa atcggcaatc tcgtggtcaa tatcatcgat
     6061 tttctgctcg atattatcgt aatgctcgac gagaatttcc ttcgcttcct gtatatctga
     6121 cgccgccggt gtcgcacctt tcaacggacg atttgccgtc tctttcatgg ttacgccggt
     6181 gattaaacca accaccgcca ctaccatcag gtaataggca ggcatcatca gattctgcga
     6241 gctttcgacc agccaggccg ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt
     6301 aaatgccgcc gccagcgcgc tgtatcggat atgcgtcggg aacatcgctg gcaaggtaga
     6361 agccataacg cccgtaaagc agttaaggat caccgccagc atcagtaacc cggcaaaaat
     6421 caggccgatg acgttactgt taatcagaat aaacgccggg atcgccaaca caaacagtgc
     6481 aacactacca agtagcacaa acggacgacg gccaaaacgg tcactcagca agcccatcac
     6541 cggctggaca aacagcatac cgatcataat agcgataata atcagcaccc cgtggtcttc
     6601 ggagtaatgc aggttatgcg acaaataact cggcatatag gtcagcaaca tgtagtaagt
     6661 cacgttggtg gcaattacca gaccaataca tgtcaacagg ctgcgccagt gtttggtggc
     6721 aatctcttta aacgagactt tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag
     6781 tttatcaaca tgctgctgga acgccggagt ctcttccagc gcatggcgca ggtaaagccc
     6841 gataatccct aacggcagag caataaagaa cggaatacgc cagccccagt cgaggaagtt
     6901 cgcttcgccg acaatggtcg aaattaacac caccacgccc gcacccagca caaacccggc
     6961 aatagaaccg aagtccagcc agctgcccat aaatccacgt ttacggtcag gtgagtattc
     7021 cgcaacaaat atcgacgccc cggtatattc accaccaacc gagaaacctt gtgccatctt
     7081 acagatcaac agcaaaatcg gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc
     7141 aatacagaac gtactgatcg acataatcac aatagtgata gcgaggatct tctggcgacc
     7201 atatttatcg cccaacatac caaagaagag tccaccaagc ggtcgaatca gaaagggaac
     7261 ggagaaagtg gcaagtgcag caaccatctg cacgctgggg tcagcccccg ggaaaaaaac
     7321 tttacctaat gcgtaagcaa caaaaccata aacaccaaaa tcgaaccatt ccattgcatt
     7381 acccagtgat gctgcggtaa tggctttacg cagtttaccg tcatcaataa tggtgacatc
     7441 acgaagggta atcggtttta cttttttcct tttcagcata gctttcctcg cagagttggc
     7501 ccatttccgc aaacgccaga aaaatcagcg cgacggccgc aaaaggggag cgggaattgc
     7561 aggatgcaat ctctgtaaca agcgtagcgg gtttgcttac accctccggt taaggaatgt
     7621 acaatcaaac cagttgacgc ctgaattggg cagttaatga cctttttacc ctaccagtgt
     7681 ttaaatttgt gatcaacttc acacatggat ttcgtttagg actcattgat gtaactgatt
     7741 atttacacca aatttacaga gaagatttcc tcgaccattg cccccgccag acgctgcgct
     7801 gaacagagtc gtggcatccc cagcgcgaca gtttgccagc gttgggtcac tgaccagcaa
     7861 acgggttgtt tatctgccgt ggtccacttt cccagccaac ttaaaaaatc agcatgatcc
     7921 ccgcccgtcg gaatggtcga agaggcaaac cactgatggt aatagtgacg cgtggtcggt
     7981 gccgcattga tactctctgc cagatgttgt gtcgctttct cgcgcaaatc acccagcaac
     8041 aacgccaccg cttgcgggct tcccagacgg caggcatcgc caaatgcccc ccagcgcaac
     8101 tcctccagaa cgataaaaca gcgcccaggc aacgaccagg cgttatacgc cccagcacgc
     8161 cagcgggtaa aaatttgctc gctctgatca cctgcctgca ccagtaggcc tcgttgcgtt
     8221 aacgcctttt ccttccagcg acaacgggct gaaaaccact catacaaggc ggcaatttgc
     8281 tgggggagct ccgggaaagc agagtgctgg cggtgatcca ttttttgcag cagcgccaga
     8341 tgttgcgcga tctgcgtcag cgccagatga cctggatcca gcatcgaagc cagtttttcc
     8401 accaatgtca gacgcaggct aagcggcgca tttaacatcc cgccgttcgt ccatgcctga
     8461 agctgcacac gcgcaagaag ttcctgcatc agtcgttcac gaaactgctg ctgatgggct
     8521 tgtggatggc gggcatcatc gccctgcgcc agatccacta aaaagcgcgg atgaaccgac
     8581 tccagcacgc gaccggggcc gtccagtaac gtcttggtca atatcgttct gccgtgaaaa
     8641 gtgtttgaat atcatcgcgt aacagctgag cgtcggtgta aatccagctg tgagtcatca
     8701 cagcctgctg caattgctgc tgcatcagcc tgaccaccga ttcattttgt tgacgcagag
     8761 ccaggctttc gcgtaaacgc gtctgtaatt ccgtcaaaca tgaagcgaac tcagcgaaaa
     8821 aagtattcat gcctgccgta acagattcat cgacctgctc tgccagaact ttagccattt
     8881 gttggcaata aagatcgact tctgcgctta atgctcgttg caacacactg taatcaaccg
     8941 tctctgtcgg ggatttctca tttccccgtc cccagtcggg ctgattcaac cagcgcgaaa
     9001 aagtctcacg cacaacgcct aaacgcgtgc tctgctcgtc cgttgcttcc tggcgcgaaa
     9061 tgacttcact gaacagctgg cgagtgttga agtggggaac tgcgccgtga aaaacaggaa
     9121 aatgaaaccc aggacgaaac cctgactcgc tcaattccat tttgatttgt tgctcaatgg
     9181 ggcgaataac atcggttaac acccggcaaa gggtggattc cagctcggca aaacgcagcg
     9241 taaagtcgcg actaatggtg ttctgcgccg tctgtaacag tgtctcacag cgggtacgaa
     9301 tctcgcttaa cggctccgaa tcatcctgaa acaaggcggc taactgcgca ttcagcgcat
     9361 cttgctgttg acgcagaaag tggttggcgg aggtcagggc cagctcgatt tcatgtttaa
     9421 tctcgccgct cacctgcgcc tgattgagtt gcaatagctg caaactttct tcgatctgat
     9481 ggatattttg ccgcaattgt tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt
     9541 attcccgcgc ctgctgcgcg taattcaaca gtttatgcgc ggcagatcgc agagcataca
     9601 acgaggcgtt agcgtaagcg gcatgaagca acgcctgaat tggctgggcg aacagcgaat
     9661 cttcccacaa ctgatcggca gcatgacgaa tatgttcgaa gtcagccaga tcggcatgac
     9721 gccagcgcct gccgagcgcg gcatgggcaa aatcttccac ccagcgttgt tgctctggcg
     9781 gtggtaactt accgttgttg gctaactcat ggcgcgcccg attcgccagg tagccccaca
     9841 tcgacgacac cggaaatatc tgctgtggcg taatacagcc tttcatcagc gtcccggaaa
     9901 tcagtgcccg cacctggtcg gcgtcgtcac tgttacgatc ctgttgatcg aacttattga
     9961 ccagcacata cagcggcacc gattgcccca ccgccaaaat cgcctcacgg acctcttcat
    10021 cggagatcga tttcagttgc gtataatcca gcaccgccag taccgccgag gcgcgagcca
    10081 gctgctggtt aagcattttt tgcagatgcg gttgcccggc ttcatttggc ccgggggtat
    10141 ccagtaacgt caactgaccg ggataactct ccagccccgc cagatggaca aactccactt
    10201 caatcacggg aatatgctca atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg
    10261 ccttcgccag tcgcaccaaa tcattcaaac ttttcagaca atgaaaaata ggctgggcac
    10321 ccagataata tttttcgaaa gcgacgccat tttcgatccg ctgcataagc gcacgcatat
    10381 ctttatctat ttccagcaca tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt
    10441 gtaatttttg aattaaacaa tcgattggcg cgacatgtga aaaatgcagt accggttcct
    10501 tttgcccggg cgtatggcga ataagcgtcg gcagcgcagt cattgggcga ttacgattag
    10561 gcaaaacctc cgtaccaaca atggcattaa tggtggttga tttccctgct ttcatggtac
    10621 cgacaatcgc cagcaccatt tccagtcggg aaattttacg caactcatta ttcagcatcg
    10681 cgtgacgttc ggcgatatta ggctgactcc agggtaaagc cagttgtggc gcatcgtctc
    10741 cggggacaga gagaggcatt ttttccagta actgcaactg ttggcgagaa agctgtaaca
    10801 ggcgttcagc ctcctgactt aactcataca gggtctgtgt gtacatagaa aattcttcct
    10861 taaagcaaat tttgttactt ttatttagcc ag
//
LOCUS       DAOTRK010000151        10631 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.152, whole genome shotgun sequence.
ACCESSION   DAOTRK010000151 DAOTRK010000000
VERSION     DAOTRK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.152"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            272..1645
                     /gene="aroP"
                     /locus_tag="QIE41_003904"
     CDS             272..1645
                     /gene="aroP"
                     /locus_tag="QIE41_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDS3532730.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1688..2542)
                     /gene="ampE"
                     /locus_tag="QIE41_003905"
     CDS             complement(1688..2542)
                     /gene="ampE"
                     /locus_tag="QIE41_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDS3532731.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKLETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2539..3090)
                     /gene="ampD"
                     /locus_tag="QIE41_003906"
     CDS             complement(2539..3090)
                     /gene="ampD"
                     /locus_tag="QIE41_003906"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDS3532732.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVNKETT"
     gene            3178..4071
                     /gene="nadC"
                     /locus_tag="QIE41_003907"
     CDS             3178..4071
                     /gene="nadC"
                     /locus_tag="QIE41_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDS3532733.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4275..4715
                     /gene="ppdD"
                     /locus_tag="QIE41_003908"
     CDS             4275..4715
                     /gene="ppdD"
                     /locus_tag="QIE41_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDS3532734.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDSGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4725..6110
                     /gene="gspE"
                     /locus_tag="QIE41_003909"
     CDS             4725..6110
                     /gene="gspE"
                     /locus_tag="QIE41_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDS3532735.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCRHCYHGF
                     YGRTALFEVLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6100..7302
                     /gene="hofC"
                     /locus_tag="QIE41_003910"
     CDS             6100..7302
                     /gene="hofC"
                     /locus_tag="QIE41_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDS3532736.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLIALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTLALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDTMSGMG"
     gene            complement(7337..8380)
                     /gene="guaC"
                     /locus_tag="QIE41_003911"
     CDS             complement(7337..8380)
                     /gene="guaC"
                     /locus_tag="QIE41_003911"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDS3532737.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8536..8580)
                     /locus_tag="QIE41_003912"
     CDS             complement(8536..8580)
                     /locus_tag="QIE41_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532738.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8605..9225
                     /gene="coaE"
                     /locus_tag="QIE41_003913"
     CDS             8605..9225
                     /gene="coaE"
                     /locus_tag="QIE41_003913"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDS3532739.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9225..9968
                     /gene="zapD"
                     /locus_tag="QIE41_003914"
     CDS             9225..9968
                     /gene="zapD"
                     /locus_tag="QIE41_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDS3532740.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQTQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9978..10175
                     /gene="yacG"
                     /locus_tag="QIE41_003915"
     CDS             9978..10175
                     /gene="yacG"
                     /locus_tag="QIE41_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDS3532741.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10263..>10631)
                     /gene="mutT"
                     /locus_tag="QIE41_003916"
     CDS             complement(10263..>10631)
                     /gene="mutT"
                     /locus_tag="QIE41_003916"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDS3532742.1"
                     /translation="IRNENNEIFITRRAADAHMANKLEFPGGKIEMSETPEQAVVREL
                     QEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPGEWMSLVGLNA
                     DDFPPANEPVLAKLKRVYVG"
BASE COUNT         2616 a         2805 c         2693 g         2517 t
ORIGIN      
        1 taattcaaaa ataatgaatt tatattcatt ttgcatattg agggaatttg aggctgacct
       61 ttacaaacgg tttcttttta agcaactcat cttcaaccat gcataaagcg ggtgcattcg
      121 ctgccgcata ccattattct tgatctgacg gaagtccttt tgtaacaatt caaacttctt
      181 tgatgtaaac aaattaatac aacaaacgga attgcaaact tacacacgca tcactgcgta
      241 gatcaaaaaa acaatcaccg cacgaggttt catgatggaa ggtcaacagc acggcgagca
      301 gctaaagcgc ggccttaaaa accgccatat tcagcttatc gcgctgggtg gcgcgatagg
      361 gaccgggcta ttcctgggta gcgcctccgt aatacagtcc gcagggccag ggattatcct
      421 gggttacgcc attgctggtt ttatcgcctt tctgatcatg cgtcagctag gtgaaatggt
      481 ggtcgaagaa cctgtcgcag gctcctttag ccactttgct tataaatact ggggcagttt
      541 tgccggtttc gcctctggct ggaactactg ggtactgtac gttttagttg ccatggcaga
      601 gcttactgct gtgggtaaat acattcagtt ctggtatcca gaaatcccaa cctgggtttc
      661 tgccgccgta ttctttgtgg tgatcaacgc catcaacctg accaacgtaa aagtgtttgg
      721 tgagatggag ttctggtttg ccattatcaa agttatcgca gtggtagcga tgatcatctt
      781 cggcggctgg ctgctattca gtggcaacgg cggcccgcag gcgaccgtta gcaacctgtg
      841 ggatcaaggt ggtttcctgc cgcacggctt caccgggctg gtgatgatga tggcgattat
      901 catgttctcg ttcggtggtc tggaactggt ggggatcacc gcagcagaag ctgataaccc
      961 ggagcaaagt atcccgaaag caactaacca ggttatctac cgcatcctga ttttctatat
     1021 tggttcgtta gccgttctgc tctcgctgat gccgtggacc cgcgttactg ctgataccag
     1081 tccgtttgta ctgatcttcc acgaattggg cgataccttt gtggcgaatg cgctgaacat
     1141 cgtggtactg actgctgcgc tctccgtgta caacagctgc gtatattgca acagccgtat
     1201 gctgtttggt ctggcacagc agggcaacgc gccaaaagcg ctggcgtctg tcgacaaacg
     1261 cggtgtaccg gttaacacta ttctggtgtc tgcgctggtt acggcattgt gcgtgctgat
     1321 taactacctt gcaccggaat ccgcattcgg actgttaatg gcgctggtgg tatctgcact
     1381 ggtaatcaac tgggcgatga ttagcctggc gcatatgaaa ttccgtcgcg ccaagcagga
     1441 acaaggcgtg gtaactcgct tccctgctct gctttatccg ctgggtaact ggatctgcct
     1501 gctgtttatg gcggcggtac tggtgattat gctgatgacc ccaggaatgg cgatttcggt
     1561 atacctgatc ccggtatggc tgatcgtgtt aggtatcggc tatctgttta aagagaaaac
     1621 cgccaaagcc gtaaaagcgc attaatctct ctacgccctc acccatacag ggtgagggca
     1681 ataatcttta caccaacgcc ccgtaaatcg tcagtagcgc aatcaccacc acgaccacga
     1741 acgaggtttt cttcgccatg gaaaccgctg ccttcggcgt ctccagctta tcgacatgcg
     1801 gttcacgcgc cagagagaac tgcgccagac gcgttaatac ctgatattgc gaagtatgga
     1861 aatcacccag cgaagcaaac caggccggta acgctttctc cccatgaccg atcaaggcat
     1921 ataccacacc cgcaagacga accggcaccc aatccagtac atgaagcacg gcatcaatgc
     1981 cggactgtaa acgatgatgc ggcgtctggt aacgcgccag ccagtattgc catgcacgca
     2041 aaaacgcata ccccatcagc gtaacgggtc cccaggttcc ccccacaatc agccagaaca
     2101 gcggtgcaag ataaaaacga aagttaatcc acagcaatgc attttgcagc tcacgcaaat
     2161 actcacgttc atcgcagcct gccgggacgc cgtgaatcat ggtgagttcg ccagccatcg
     2221 tggcacgggc atggctatca ttacgtgaag cagctgtcag ataagcatga taatgaagac
     2281 gaactttacc tgcgccaata cacagcaaac caatcagcag ccacaccagc agcgtgggaa
     2341 cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa agtcacgccc atcgcaataa
     2401 tggtcatgcc taatgtgcgc ccgagagaaa aatgtttcac ccgccgaaag aacgcttcaa
     2461 gacgatgatc aagctgccag tgctcgccca acttaaacag gcgctcgaaa attaacacca
     2521 gtaaggttgt aaatagcgtc atgttgtctc cttgttgacc agcgcacgaa accgtgccca
     2581 atcaaatgca ggaccgggat cggttttccg atccggcgca atatcacaat ggcccgtcat
     2641 gttattagcg atatctggat agcgatcaat cagtgcccgc gtaaccgccg caagctgttg
     2701 atactgcgca tcggtatacg ccagcgtatc ggtgccttcc agctcaatcc caatagaaaa
     2761 atcattgcag cgttcgcgcc cctgatactg agagactccg gcatgccagg cacgtttatc
     2821 gaaaggaaca tactggacta tttcaccatc gcggcgaatc aaacagtgag cggagacgcg
     2881 caaatgggcg atctcagcaa agaaaggatg tgcctgcgga tcaatagttc cagtgaataa
     2941 tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg ctaatattgt gcaccaccag
     3001 cagggtgggt gtttcgtcat ccgggcggca atcgtaatgt ggtgagggaa cgcggcgcgc
     3061 gccaaccagc cacccctgtt ctaacaacat gcaggatctc cttatatgtg gtgctaatac
     3121 ccggttcaga gtagcatgtt tctaccttat gattcgttag ctatctggag ttttaacatg
     3181 ccgcctcgcc gctataaccc tgacacccga cgtgacgagc tgctggaacg cattaatctc
     3241 gatatccccg gcgcggtggc ccaggcgctg cgggaagatt taggcggaac agtcgatgcc
     3301 aacaatgaca ttacggcaaa acttttaccg gaaaactctc gctctcatgc cacagtgatc
     3361 acccgcgaga atggcgtatt ttgcggcaaa cgctgggttg aagaggtgtt tattcaactg
     3421 gcaggcgacg atgtcaccat aatctggcat gtggatgacg gcgatgtcat caacgccaat
     3481 caacccttgt tcgaacttga aggcccatcc cgcgtgctgt taacgggcga acgcactgcg
     3541 cttaattttg tgcaaaccct ttcaggggtt gccagtaagg tacgccacta tgtcgaatta
     3601 ctggagggca ccaacacgca gttgttggat acgcgcaaaa ccttacccgg cctgcgttca
     3661 gctctgaaat acgcggtact ttgcggcggc ggagcgaatc accgtctggg gctttctgat
     3721 gcctttctga tcaaagaaaa ccatattatt gcctccggct cagtgcgcca ggcggtcgaa
     3781 aaagcatcct ggctgcaccc ggatgcgcca gtagaagtcg aagtagagaa tctggaagaa
     3841 cttgatgaag ccctgaaagc aggtgccgat atcatcatgc tggataactt cgaaacagaa
     3901 cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc tactggaagt gtctggcaac
     3961 gtcactgaca aaacactgcg cgaatttgcc gaaacgggcg tggactttat ctccgttggt
     4021 gcactaacta aacacgtaca ggcactcgac ctttcaatgc gttttcgcta attgctaaca
     4081 gatttatccg ctccatcgcg ggcggataaa tctccccttc cccaactttc ttcgtaacgc
     4141 ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaagc tgctttcccg
     4201 tttcgttttt tgcccctcgc ttgtgctgat ggcgtttgcc aaagtagcgc caaccaaatc
     4261 aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta
     4321 ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca
     4381 aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt
     4441 gcgcgctgga acatggtgga ttaaatacct gcgacagtgg cagcaatggc attccctcgc
     4501 ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg
     4561 ggcaagaaag tctcaatggg ctaagcgtcg tcatgacacc gggttgggat aacgcaaacg
     4621 gcgtcaccgg ttggacgcgc aactgcaata ttcaaagtga cagcgcattg cagcaagcct
     4681 gcgaagatgt cttccgtttt gatgacgcca actaaggagc ggcaatgaat attccacagc
     4741 tcaccgccct gtgtctgcgt tatcagggag tcttgctgga tgccagcgaa gaggtggttc
     4801 atgttgcggt ggtcgatgcc ccttcgcatg agctactgga cgcattgcat ttcgctacca
     4861 ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac gccagtcgca
     4921 cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctga
     4981 ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc gaaccagcgg
     5041 acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt
     5101 caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggcaac ctggatattg
     5161 cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct
     5221 catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta agattgttac
     5281 agcaggtgag tcaggcactg gatgtcaaca cgcttggaat gcagccgtta caactggcgg
     5341 actttgctca tgccttgcaa caaccacagg gactggtgct ggtaacaggc cctacaggca
     5401 gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct gaatactgct gatattaata
     5461 tttgtagcgt cgaagatccg gttgagatcc ccatagccgg actaaaccag acgcaaatcc
     5521 atccgcgtgc cggtcttacc tttcagggcg ttttgcgtgc gttattgcgc caggaccctg
     5581 acgtcattat gatcggagag atccgcgatg gcgaaacagc agagatcgct attaaagcgg
     5641 cgcaaaccgg tcacctggtg ttgtctaccc tacacaccaa ctccacctgc gaaacgctgg
     5701 tgcgtttaca gcaaatgggc gttgctcgct ggatgctctc atcagcgctt acgctggtaa
     5761 tagcccagcg tctggtacgc aaactttgcc cacattgtcg ccgacagcaa ggggagccaa
     5821 tccatattcc agacaatgta tggccgtcgc cgctgccccg ctggcaagcc cctggttgta
     5881 gacactgcta ccacggtttt tatggtcgta cggccttatt tgaagttcta cccataacac
     5941 cgatcatacg ccagcttatt tccgctaata ccgacgttga atcgctggaa acacacgccc
     6001 gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa
     6061 ccacctttga agagttaatc cgcgtattgg ggatgccgca tggcgagtaa gcaactctgg
     6121 cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggctgagagc
     6181 cgtgctttgc tgctcatagc actacagcaa cagatggtta ccccacttag cctgaagcga
     6241 atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg
     6301 gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggcactgct ggcggaacag
     6361 catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct tgaacagggc
     6421 gttgcctttt ccaatgcctt attaccctgg tcagaggtat ttccgccgct ctatcaggcg
     6481 atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt
     6541 cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatt
     6601 atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctaccggag
     6661 tttgccgcta tctataagac ctttaacact ccgctaccgg cgctaacgca ggggatcatg
     6721 acgctggcaa gctttagtgg cgaatggggt tggttgctgg tgctattcgg ctttctgctg
     6781 gcgatagcca ataagctgct gatgcgccac ccaaccaggc ttatcgcacg gcagaaattg
     6841 ctgttacgca tcccgattat gggttcactg atgcggggac aaaaactcac acagatcttt
     6901 acgattctgg cgctgacaca aagtgcaggc attacttttt tacagggcgt agagagcgtc
     6961 agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc
     7021 agtaacggtc aacccatctg gctggcgcta aaaaatgccg gtgagtttag cccactctgt
     7081 ttgcaattag tgagaacagg agaggcatcc ggctcgctgg atctcatgtt agacaacctc
     7141 gcccatcatc atcgggataa cacactggcg ctggcggata acctcgcagc cttactggaa
     7201 ccggcgttac tgatcataac gggaggaatt atcggtacgc tggtggtggc aatgtatctg
     7261 cccattttcc atttaggcga tacaatgagt ggaatgggat aacgctggcg tgttgctcca
     7321 cgccagcgtt gggagattac aggttgttga agatgcggtt ttcttgttcc agcacacgga
     7381 taaatgtggt gcgcttggtc agctctttca ggcgtgaagc cccaacgtat gtacaagctg
     7441 aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct
     7501 taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg
     7561 cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc
     7621 gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc
     7681 cgccaaaggc tttcgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc
     7741 cgcccagacc atgcgcagca tcggcacatt cgatcaccgc agaaagttgc ggataaccga
     7801 cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat
     7861 cagcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa
     7921 tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat
     7981 aaccattcgc cacgtcaata caaacgaaat ttaatgccgg gttcaggtcg agaatctgtt
     8041 tagttttttc gaaatccgca tctgatgtac cggtagaaac catcacatgt ttcagcacat
     8101 cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag
     8161 cagtcaaaat atcaaaagaa gccagcgcag aggccataga aaatgtgcct acggtgtcca
     8221 tatttgcggc gataatcggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt
     8281 gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa
     8341 cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa
     8401 tggctataag ggtaagagcg caactccagt gacgttatca tacgcactaa tcaatgcggc
     8461 gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag
     8521 tgagcgaacg tttcatcaat ttcgctttcg tgcgctcatt tttaacgttt tgataatcat
     8581 gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc
     8641 agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc
     8701 gatattattg cgcgtcaggt ggttgaacca ggcgcacctg cgctacatgc cattgctgat
     8761 cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag
     8821 cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt
     8881 cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact atgggttgtg
     8941 ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc
     9001 agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtt
     9061 gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc
     9121 attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac
     9181 tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc
     9241 tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc
     9301 agcaactcac cgttaatcta cccatcgttg accacgctgg cgcgctgcat ttcttccgta
     9361 atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag
     9421 aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga
     9481 gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc
     9541 cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga
     9601 gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac
     9661 cccagacgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc
     9721 aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca
     9781 gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgt ctgaatctgt
     9841 cgctcgattc acagctttat ccgcaaattt ccggtcataa gagccgtttt gccattcgtt
     9901 ttatgccgct ggacagtgaa aacggacagg taccggaacg tctggatttc gaactggcct
     9961 gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa
    10021 cggtggtgtg gggcgaaatc agcccgtttc ggccattttg ttccaaacgt tgccagctga
    10081 tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg
    10141 aaagcgatga ctggagcgaa gagccaaagc agtgacattt tgtctgatgc gacgctggcc
    10201 tgataagacg cgccagcgtc gcatcgggca tcttgtgcaa atgttggatg cggcataaac
    10261 gcctatccga catatacacg cttaagcttc gcaagtaccg gttcattggc tggcggaaaa
    10321 tcatcggcat tcagaccgac cagcgacatc cactcaccgg gttgcccttc tttaccccac
    10381 ggctcccctt cccagctttc gaccagccag aaccacagag ttatatgcct gtccgggaat
    10441 tcatattcca gtttttcaaa tagcgaaaaa tgttgcgggg taatcccgac ttcctcctga
    10501 agttcacgca ccaccgcctg ttccggcgtt tcgctcattt caattttacc gccgggaaac
    10561 tctagtttat tcgccatgtg cgcatctgct gcgcgacggg ttataaagat ttcattgttc
    10621 tcgttgcgaa t
//
LOCUS       DAOTRK010000152        10534 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.153, whole genome shotgun sequence.
ACCESSION   DAOTRK010000152 DAOTRK010000000
VERSION     DAOTRK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.153"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            202..2343
                     /locus_tag="QIE41_003917"
     CDS             202..2343
                     /locus_tag="QIE41_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDS3532743.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2407..4299)
                     /gene="parE"
                     /locus_tag="QIE41_003918"
     CDS             complement(2407..4299)
                     /gene="parE"
                     /locus_tag="QIE41_003918"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDS3532744.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4328..4909)
                     /gene="yqiA"
                     /locus_tag="QIE41_003919"
     CDS             complement(4328..4909)
                     /gene="yqiA"
                     /locus_tag="QIE41_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDS3532745.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4909..5736)
                     /gene="cpdA"
                     /locus_tag="QIE41_003920"
     CDS             complement(4909..5736)
                     /gene="cpdA"
                     /locus_tag="QIE41_003920"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDS3532746.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5761..6183)
                     /locus_tag="QIE41_003921"
     CDS             complement(5761..6183)
                     /locus_tag="QIE41_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDS3532747.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6184..6813)
                     /gene="nudF"
                     /locus_tag="QIE41_003922"
     CDS             complement(6184..6813)
                     /gene="nudF"
                     /locus_tag="QIE41_003922"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDS3532748.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSKTPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7018..8499
                     /gene="tolC"
                     /locus_tag="QIE41_003923"
     CDS             7018..8499
                     /gene="tolC"
                     /locus_tag="QIE41_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDS3532749.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8647..9318
                     /locus_tag="QIE41_003924"
     CDS             8647..9318
                     /locus_tag="QIE41_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDS3532750.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9324..10484
                     /locus_tag="QIE41_003925"
     CDS             9324..10484
                     /locus_tag="QIE41_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDS3532751.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPLFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2658 a         2807 c         2586 g         2483 t
ORIGIN      
        1 atcaattgaa ttgcattaac ctctcataaa aacacatgta gcaatttttt gttatttctc
       61 atttactact acatcaccgc atggttgaat gattctcaat tacatatccg atggcgaaat
      121 aacaaacgga tagtgcttag tgagcaacaa gaaacaattt tcactcacga caatccgtga
      181 gtttttggga taacagacta aatggctaag ttcacacctt cattctcagg aatcaaaggt
      241 cgggcgctct tttcactgct ctttgcagca ccaatgattc atgcaaccga cactgcaacg
      301 accaaagatg gcgaaacaat cactgttaca gcggatgcaa ataccgcaac tgaggcgacc
      361 gatggttatc aacctctgag cacttccacg gcgacattaa ccgatatgcc gatgctggat
      421 atcccgcagg tggtcaatac ggttagcgat caggttctgg aaaatcagaa tgcgacgacg
      481 ctggatgaag cgctttataa cgtcagtaac gtggtacaga ccaatacatt aggcggaact
      541 caggacgcct ttgtacgtcg tgggtttggt gctaaccggg atggctccat catgaccaac
      601 ggcctgcgaa ctgtacttcc tcgcagtttc aacgccgcca cagaacgtgt ggaagtgcta
      661 aaaggtccgg cctccacgct gtatggcatt ctcgatcctg gtggattgat taacgtcgtg
      721 accaagcgcc cggaaaaaac attccatggt tctgtctcag ccacgtcctc cagttttggc
      781 ggaggcactg ggcaacttga tatcacaggt cccattgaag gcactcagct ggcataccgc
      841 ctgacggggg aagtgcagga tgaagattac tggcgaaact tcggtaaaga gcgcagtaca
      901 tttattgccc cgtcactcac ctggtttggt gataatgcaa cagtaaccat gctctattcc
      961 catcgggact ataaaactcc gttcgatcgt ggaacgattt tcgaccttac gacgaaacag
     1021 cccgtaaacg ttgatcgaaa aatacgtttt gacgaaccgt ttaatattac agatggtcag
     1081 tccgatctgg cgcaactcaa cgcagaatat catctcaata gccagtggac agcgcgcttt
     1141 gattacagct acagccagga taaatacagc gacaatcagg ctcgcgttac cgcgtatgat
     1201 gcaacgacag gaacgctgac acggcgtgtt gatgcaactc agggatctac ccagcgtatg
     1261 cattctactc gtgcggatct gcaagggaat gttgatattg ctgggttcta taatgagatt
     1321 ctgggtgggg tgtcatatga atattatgat cttctgcgca cagatatgat tcgctgtaaa
     1381 aacgctaaag atttcaatat ctacaaccct gtttacggca ataccagcaa atgcacaact
     1441 gtctcggcgt cggacagcga tcagacgatc aaacaggaga gctactcagc ttatgcacag
     1501 gacgcgctct atctgaccga taactggatt gccgtcgccg ggatccgcta tcagtattac
     1561 acgcagtatg cgggtaaagg ccgtcctttt aatgtcaata ctgacagccg cgatgaacaa
     1621 tggacgccca aactggggtt agtctacaaa ctgacgccat cggtatcctt atttgctaat
     1681 tattcgcaaa catttatgcc gcaatcgtca attgccagct acatcggaga tcttccaccg
     1741 gaatcatcta atgcttacga agtcggggca aaattcgagc tgttcgatgg tataaccgca
     1801 gatattgcgc tgtttgatat ccataaacgt aacgtgttgt ataccgaaag tattggtgat
     1861 gaaaccatcg ccaaaacggc aggccgcgtt cgttcaagag gggtagaagt cgaccttgcg
     1921 ggagcattaa ctgaaaacat taatatcatt gccagctacg gctataccga tgctaaggtt
     1981 ctggaggatc ctgattatgc agggaaacca ttgccgaatg ttcctcgtca taccggttcg
     2041 ctattcctga cctatgacat tcataacatg ccaggcaata acacactgac gtttggcggt
     2101 ggtggacatg gcgtaagccg tcgttcggca accaatgggg ctgactatta tctgccaggc
     2161 tatttcgttg ccgatgcctt cgccgcatac aaaatgaaat tgcagtatcc ggtcacactg
     2221 caattaaacg tcaaaaacct gtttgataaa acgtattaca cctcttccat cgccacaaat
     2281 aatctgggga atcagattgg cgatccgcgt gaagtgcaat tcacggtgaa aatggaattt
     2341 tgatcaaaaa agcccggtat acacttgtat accgggctat gttttattct tctggcggga
     2401 ggattatcag acctcaatct ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc
     2461 ttccgagcgt ttcttcgcca gcagcatatc catcatcgcg tcagtacgct gatcgtcttc
     2521 atcatcgata gtcagctgca ccagacggcg agtgttcgga tcaagcgtgg tttcgcgcaa
     2581 ttgcatcggg ttcatttccc ccagaccttt aaaacgctga acgttcggct tgcctttctt
     2641 gcgttttaat tgctcaagta cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc
     2701 tttcccgaga tcaatacggt agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac
     2761 caatgcgcgg aagtgtttta caaacaaagc gcagagcagc gtggcaatgt gcagaccatc
     2821 agagtccgca tccgcgagga tacagatttt gccgtaacga agctggctca gatcgtcgct
     2881 gtcaggatcg ataccgatcg ctaccgaaat atcgtgcact tcctgcgaag ccagcacttc
     2941 gtcggaggag acttcccagg tgttaaggat cttacctttc agtggcatga tagcctgata
     3001 ttcgcgatcg cgcgcctgct tggcagatcc gcctgcggag tcaccttcca caaggaacag
     3061 ctcggtacgg ttaaggtcct gcgcggtaca atcagccagt ttgccaggca acgccgggcc
     3121 gctggttagc tttttacgca ccactttttt agccgcacgc atacgacgct gggcgctgga
     3181 aatcgccatc tctgccaaca gctccgccgc ctgaacgttc tggttcagcc acaggatgaa
     3241 ggcatctttc accacgcccg aaacgaatgc cgcgcactga cgcgaagaga gacgttcttt
     3301 ggtctgcccg gcaaactgcg gatcctgcat ttttactgac agcacatagg cgcagcgatc
     3361 ccagatatct tccgccgaca gctttacacc gcgcggcaga atgttgcggt attcacagaa
     3421 ctcacgcatc gcgtccagca ggccctgacg caggccatta acgtgcgtac cgccctgcat
     3481 cgttgggatc aggttgacgt agctttcggt cagcagttca ccgccttccg gcagccacag
     3541 tagcgcccag tccaccgctt cagtatcgcc agcgaaatta ccgataaacg gtttttctgg
     3601 cagcgtcggt aaaccgttta ccgcttccgc caggtaatca ttcagaccgt cctgatagca
     3661 ccagcgctgt tcggtgttgt tgatctcatc tttaaaggtg atctcaacac cagggcacag
     3721 taccgctttg gctttcagca catgtgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa
     3781 gaaggtttca tccggccaga agtgcacgct ggtaccggta ttgcgcttac cgcaagtgcc
     3841 gacaacctgt aaatcctgca ccttttcgcc attttcaaag gcgatgttat aaacctgacc
     3901 atcgcggcgt acgttaactt ctacgcgctt cgacagggcg ttaactaccg aaatccccac
     3961 gccatgcagg ccgccagaaa actggtagtt tttgttagag aatttaccgc ctgcatgcag
     4021 acggcaaaga atcagttcaa ccgccggtac accctcttcc gggtgaatat ccaccggcat
     4081 ccctcgccca tcgtcaatca cttctaacga ctggtcagca tgtaagatta catccacgcg
     4141 ttttgcgtga cccgccagtg cttcatcgac actgttatca ataacttctt gccccaaatg
     4201 gttagggcga gtggtatcgg tatacatccc cggacggcgg cgaaccggct caagcccggt
     4261 gagtacctca atggcatcag cgttataagt ttgcgtcatg gtttaagtta gcaattcgag
     4321 ttgatcgtca gagatggtgc agaccaagaa aatcgacgat cgggttgaaa taatcttcga
     4381 agcccgtgaa tgcgtggttg ccgccttcta tgacagtctg gcggcaggaa gcgtagtacg
     4441 ccaccgcctg gcggtaatcc agcacttcat ctcccgtctg ttgcagcagc cagatcaaat
     4501 ccggcgcttc cagcgggtca atctgcatga ctttgagatc gtaaatatgg cgtgactcta
     4561 gcacatattg ctgcccggtg taggggttct cgttctgacc gagatagtcc gtcagcagtt
     4621 caaacgggcg caccgccggg ttgaccacca ctgcgggcag cataaaacat tgcgacaacc
     4681 aggtggcgta atatcccccc agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt
     4741 ccaggacaat ggattccagc agttctgccg catcggaagg atacggcggc aactgcggaa
     4801 tgatcatctc tacgtcaggg tgatgttccg ccaaccagtt ttttaacaag ctcgcttttg
     4861 cagagcgcgg cgagctgttg aaaccgtgta aataaagaag cgtagacatc agtagccttc
     4921 tgaagcggta tcaggttgga aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt
     4981 gccatcagca tgtaactcga gagtacgcca gccgggcgcg atggtgtcca gcgtaaagtt
     5041 ggaacagtgc ggcttaaact gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt
     5101 ccagtcgaga tccagctcct gatgaatatg accgcacagc aagtatttga catgcggaaa
     5161 cttcgccagc acggtatcca gttcgcccgc gttacgcagg ctgtgttgat caagccaact
     5221 acaacccgca ggtaacggat gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc
     5281 ggccagttta cgttccagcc actcaagctg aaactcgctc agctcaccgt gcggcacgcc
     5341 aaacacctgg ctatccagca acaggatttg ccattgctca ccaataaaca cgcgcttcgc
     5401 cggggagata cccgcatcct gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc
     5461 cggcagccag acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gctgataggc
     5521 cgcagaggat tgatcctgcg ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg
     5581 gtgtggccga atcgcctcca gcaccgcctg gtagctttcc caggtgttta cccctaacag
     5641 ggcttcgtgc ttttgcgcaa acaggtgagt gtcggtaatt tgtaaaatcc tgactctggc
     5701 ctcaccagcc agaggaaggg ttaacaggct ttccaaatgg tgtccttagg tttcacgacg
     5761 ctagtaaacc ggaatcgcca tcgctccatg tgctaaacag tatcgcaacc agtccgctaa
     5821 aaactggtta atttgatgct tttcgtcgcg ttgatgcaac tttttattag gataatcata
     5881 ccgcgctttg aagcgaaaaa tctgctggct tgaacacact tcagccacca tcgcgtcatg
     5941 atacagacgc accgtcattg acggaaggct ccagtaactg atcgcgggcg cagtctgttc
     6001 tattgtcacc agggtagtgt atcgggtcga ttccacaatc gtcagccgat attgtgcgtt
     6061 tgccacctga tagcttacag tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa
     6121 ttgtgaaaag ttcatctcgc acaggcgcat catttcagga aagtcaggtg tgtaacgctt
     6181 catttatgcc cactcatttt ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat
     6241 gaccgacgct gcgttgtcga ttttcccctc ttctacccac tggtatgcct gttcccggct
     6301 taccacatga acgcgaatat cttcgttttc atcagccaga ccgtgaatac cgcttgcggt
     6361 cgtggcgtcc acttcgccca ccataattga cgaacgctca ctggtgcccc ccgggcttgc
     6421 caggaaactt aacaccggtt tggtccgttt gactatcagt cccgcctctt caatcgcttc
     6481 gcgacgggca acatcttcca cactttcacc ctcttcaatc atcccggcaa ccatctccag
     6541 tagccagggg gttttgctgg tgtcgtacgc ggcaattcga atctgctcaa tcagcacaac
     6601 ttcatcacgc actgggtcaa agggtagcaa gactgcggcg tgaccgcgct caaaaatttc
     6661 ccgccgtacc tcatgactca tttgcccgtt gaatagacga tgacgaaatc tataaagatc
     6721 taatgaaaaa aagccgcgat aaagtgtttc tcgtgcaata atttctacat cgtttttgcc
     6781 aaatgtaacg ggcaggttgt ctggcttaag cattgttaat gtcctggcac taatagtgaa
     6841 ttaaatgtga atttcagcga cgtttgactg ccgtttgagc agtcatgtgt taaattgagg
     6901 cacattaacg ccctatggca cgtaacgcca accttttgcg gtagcggctt ctgctagaat
     6961 ccgcaataat tttacagttt gatcgcgcta aatactgctt caccacaagg aatgcaaatg
     7021 aagaaattgc tccccattct tatcggcctg agcctctctg ggttcagttc gttgagccag
     7081 gccgagaacc tgatgcaagt ttatcagcaa gcacgcctta gtaacccgga attgcgtaag
     7141 tctgccgccg atcgtgatgc tgcctttgaa aaaattaatg aagcgcgcag tccattactg
     7201 ccacagctag gtttaggtgc agattacacc tatagcaacg gctaccgcga cgcgaacggc
     7261 atcaactcta acgcgaccag tgcgtccctg cagttaactc aatccatttt tgatatgtcg
     7321 aaatggcgtg cgttaacgct gcaggaaaaa gcagctggga ttcaggacgt cacgtatcag
     7381 accgatcaac aaaccttgat cctcaacacc gcgaccgctt atttcaacgt gttgaatgct
     7441 attgacgttc tttcctatac acaggcgcaa aaagaagcga tctaccgtca attagatcaa
     7501 accacccaac gttttaacgt gggcctggta gcgatcactg acgtgcagaa cgcccgcgca
     7561 cagtacgata ccgtgctggc gaacgaagtg accgcacgta ataaccttga taacgcggta
     7621 gagcagctgc gccagatcac cggtaactac tatccggaac tggcggcgct gaatgtcgaa
     7681 aactttaaaa ccgacaaacc acagccggtt aacacgctgc tgaaagaagc cgaaaaacgc
     7741 aacctgtcgt tgttacaggc gcgcttgagc caggacctgg cacgcgagca aattcgccag
     7801 gcgcaggatg gtcacttacc gactctggat ttaacggctt ctaccgggat ttctgacacc
     7861 tcttatagcg gttcgaaaac ccgtggtgcc gctggtaccc agtatgacga tagcaatatg
     7921 ggccagaaca aagttggcct gagcttctcg ctgccgattt atcagggcgg aatggttaac
     7981 tcgcaggtga aacaggccca gtacaacttt gttggtgcca gcgagcaact ggaaagcgcg
     8041 catcgtagcg tcgtacaaac cgtacgttcc tccttcaaca acattaatgc ttctatcagt
     8101 agcattaacg cctacaaaca agccgtagtt tccgctcaaa gctcattaga cgcgatggag
     8161 gcgggttact cggtcggtac gcgtaccatt gttgatgtgt tggatgcgac caccacgctg
     8221 tacaacgcta agcaagagct ggcaaatgcg cgttataact acctgattaa ccagttgaat
     8281 attaagtcag ctctgggtac gttgaacgag caggatctgc tggcactgaa caatgcgctg
     8341 agcaaaccgg tttccactaa tccggaaaac gttgccccgc aaacgccgga acagaatgct
     8401 attgctgatg gttatgcgcc tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc
     8461 actaccacca gtaacggtca taaccctttc cgtaactgat gactacgacg gggcttcggc
     8521 cccgtctaaa cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct
     8581 cgcttcaatt tcgaccagcc atcctctatt ctgatgggta tttaccactg gtcccggaag
     8641 acaaaaatga aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca
     8701 cgccatctga caccagtcgc tctcgcggtt gccactgttt ttatgctggc tggctgtgaa
     8761 aagagtgatg aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca
     8821 ggcaaaagcg ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact
     8881 gcgccgaaat acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgccag
     8941 caggcaccag cccaggctgg catggcacca gaaaaccagg cgcaggccca gcaatccagc
     9001 gggagtttct ggatgccgct gatggccggt tacatgatgg gtcgtctgat gggcggcggc
     9061 gcgggatttg cacagcagcc gctgttctcc tcgaaaaacc cggccagccc ggcttacggt
     9121 aaatataccg acgcgaccgg taaaaactat ggcgcagccc agccaggccg caccatgacc
     9181 gtaccgaaga cggcaatggc accaaaaccg gcaaccacca ccaccgttac tcgtggcggt
     9241 tttggtgaat ctgttgccaa acaaagcact atgcagcgta gcgcaaccgg cacctcttct
     9301 cgttcaatgg gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc
     9361 gcgaaaaagc ccacgaatac ggtttcaatt ttcacaccat gtacggcgaa ccgtactggt
     9421 gtgaagatgc ttactacaag ttgaccctcg cccaggttga aaagctggaa gaagtcaccg
     9481 ccgaactgca ccagatgtgc ctgaaagtgg ttgaaaaagt gatcgccagc gatgagctga
     9541 tgaccaaatt ccgcattcca aaacacacct ggagttttgt gcgccagtca tggctgactc
     9601 accagccatc gctttattcg cgtcttgatc tggcgtggga tggtactggc gaacctaaac
     9661 ttctggaaaa taacgccgat acgccaacgt cactgtatga agccgcattc tttcagtgga
     9721 tctggctgga agatcagctt aacgccggta acctgccgga aggcagcgac cagtttaaca
     9781 gtctgcaaga aaagctaatc gatcgctttg tcgagttgcg tgaacagtat ggcttccagt
     9841 tgctgcatct cacctgctgt cgcgacacgg tggaagatcg cggaaccatc cagtatttgc
     9901 aggactgcgc gacagaagct gaaatcgcta ctgaattcct ctacatcgac gatatcggtt
     9961 taggcgaaaa aggtcagttc acggatttac aagatcaggt gatttctaac ctgttcaagc
    10021 tgtatccgtg ggaatttatg ctgcgtgaga tgttctccac caagctggag gatgcaggcg
    10081 tacgctggct ggaaccggcg tggaagagca ttatctccaa caaagcgctt ctaccgctgt
    10141 tgtgggagat gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc
    10201 atccgcaaat ggaaaaatat gtggttaaac cgctcttctc ccgtgaaggc gcaaacgtgt
    10261 cgatcattga gaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga
    10321 tgattgttca gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta
    10381 gctggctggt gaacgatcaa cctgccggaa ttggcattcg tgaagaccgt gcgttgatca
    10441 cccaggatat gtctcggttt tatccgcata tttttgttga gtaatactgt tcaatattgc
    10501 cccgtttacc ggggcaattt attatacttc gcaa
//
LOCUS       DAOTRK010000153        10488 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.154, whole genome shotgun sequence.
ACCESSION   DAOTRK010000153 DAOTRK010000000
VERSION     DAOTRK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.154"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..813
                     /locus_tag="QIE41_003926"
     CDS             <1..813
                     /locus_tag="QIE41_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS3532752.1"
                     /translation="GWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTENEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKCK
                     IQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAK
                     SGLNRTILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSK
                     FRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEIIQATA"
     gene            857..964
                     /locus_tag="QIE41_003927"
                     /pseudo
     CDS             857..964
                     /locus_tag="QIE41_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(948..1586)
                     /gene="ytfB"
                     /locus_tag="QIE41_003928"
     CDS             complement(948..1586)
                     /gene="ytfB"
                     /locus_tag="QIE41_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDS3532753.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNALVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1804..2424
                     /gene="fklB"
                     /locus_tag="QIE41_003929"
     CDS             1804..2424
                     /gene="fklB"
                     /locus_tag="QIE41_003929"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDS3532754.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2733..4145
                     /gene="cycA"
                     /locus_tag="QIE41_003930"
                     /gene_synonym="dagA"
     CDS             2733..4145
                     /gene="cycA"
                     /locus_tag="QIE41_003930"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDS3532755.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPELSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(4190..4852)
                     /gene="ytfE"
                     /locus_tag="QIE41_003931"
     CDS             complement(4190..4852)
                     /gene="ytfE"
                     /locus_tag="QIE41_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDS3532756.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQASKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4960..5925)
                     /locus_tag="QIE41_003932"
     CDS             complement(4960..5925)
                     /locus_tag="QIE41_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDS3532757.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFVSLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLIEPVSES"
     gene            complement(6034..6894)
                     /gene="qorB"
                     /locus_tag="QIE41_003933"
     CDS             complement(6034..6894)
                     /gene="qorB"
                     /locus_tag="QIE41_003933"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDS3532758.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQSLTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6983..7363
                     /locus_tag="QIE41_003934"
     CDS             6983..7363
                     /locus_tag="QIE41_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDS3532759.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(7481..9424)
                     /gene="cpdB"
                     /locus_tag="QIE41_003935"
     CDS             complement(7481..9424)
                     /gene="cpdB"
                     /locus_tag="QIE41_003935"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDS3532760.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRVGKIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9614..10354
                     /gene="cysQ"
                     /locus_tag="QIE41_003936"
     CDS             9614..10354
                     /gene="cysQ"
                     /locus_tag="QIE41_003936"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDS3532761.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPAFRVSIY"
BASE COUNT         2461 a         2668 c         2757 g         2602 t
ORIGIN      
        1 ggctggatgc gctaccggaa tagccgtcag gtcacgggtg ttgtgaaaaa tgtcacggtc
       61 agccagtcct gcggtaagtg gtacatcagt attcagactg aaaatgaagt atcaactccg
      121 gttcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact cgccacgctg
      181 tcagatggca cagtctttgg gcctgtaaac agttttcaga aaaaccagaa gacgctggcg
      241 agacttcagc ggcagttaag ccgcaaggtc aaattcagca acaactggca gaagcagaaa
      301 tgcaaaatac agcgactgca ttcccgtatc gcaaatatcc gcagggacta ccttcacaaa
      361 gtcacaacga ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc
      421 aacatgtcaa agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa
      481 tcaggtttaa accgtacgat actggatcag ggctggtatg aaatgcgccg ccagcttgag
      541 tacaagcagc tctggagtgg cggtcaggtg cttgctgttc cgccagcgta cacaagccag
      601 cgttgcgcgt gctgtggtca tacagcgaaa gaaaatcgcc tgtcacaaag taaattcaga
      661 tgccaggtat gtggatatac agcgaacgcc gatgtaaacg gcgctcgtaa cattttagcg
      721 gcgggacacg ccgttcttgc ctgtggagag atggtgcagt caggccgctc gttgaagcag
      781 gaacccaccg aaataattca ggcgacagcc tgaacgtagc agggaaccac gtccttcagg
      841 gcgtggagga tgtcaacaca tcagtatggt ttcaccccag gcactggaag ccagacgtaa
      901 ggtcatcaaa gagacaatcc ttagctggct tttggttgac ccttcatcta ccgcgcacga
      961 ataaaactgc catccggctg gcgggtaaac aagacctgtt gattattccc cgtatcaatg
     1021 gttaagcccg tcaccacgcc gctggcgttc tggcggattt tcaccatctg cccgttttgc
     1081 aaattactca gcggcttacc cgcgccttca acctgcgcca ttgcatagac atcggtagcg
     1141 ggcagtccgt ggtcgcggaa cagctgcgcc atcgttttac cgggttcgac acggtaagag
     1201 cgccattgat tatcaattcc gctatctggc tggaatggtt gcgtttgggt ggtttgcggc
     1261 tgctcttctg gctggccttc ctgtattggt tctggagcga ccggggccac ctgatcagga
     1321 tcattttgtg gcgtaactag ctgcgcccgc agctgttctt ctgtcggtgg ttgtgattgc
     1381 gactgaatat ctaactgcgc ttcacgcgtc accaccagcg cgttgggcgt atcatcagat
     1441 ggaagcagaa atccgaccac caatacaatg gcggcaataa tgatcccccg acgatgcatc
     1501 ggcggcagcg ggtccatgaa gcgaaaattg tctggcgcgt gccagacttt ctccagggtt
     1561 ggttttagtt caaagcgccc gggcatggtt tcctcctgct ccgcgtcttg ttcctcaatc
     1621 atagcctatg aataagctaa cgctatgatg tccgtggtaa acccgccttt attatattca
     1681 tacgggatat tgctattgtt tctttttccc cgggatttgt catcattccc gcgacaaagt
     1741 tttacccaaa gaagtgtggc tgatatgctg cccgctactt tataccctaa gaaaggaaat
     1801 acgatgacca ccccaacttt tgacaccatc gaagcgcaag caagctacgg cattggtttg
     1861 caggtagggc aacaactgag tgaatctggc ctggaagggc tgctgccaga agcactggtt
     1921 gcaggcattg ctgatgcgct ggaaggcaaa catccggctg ttccggttga tgtggtgcac
     1981 cgcgcgctgc gtgaaatcca cgaacgcgcc gatgccgttc gtcgtcagcg tttccaggcg
     2041 atggctgctg aaggtgtgaa atacctggaa gaaaacgcca aaaaagaagg tgtaaatagc
     2101 accgaatctg gcctgcaatt ccgcgtgatc aaccagggcg aaggcgcaat tccggcacgt
     2161 accgaccgcg ttcgtgttca ttacaccgga aaactgatcg acggcaccgt gtttgacagc
     2221 tccgttgctc gcggcgagcc agctgaattc ccggttaacg gtgtgatccc tggctggatt
     2281 gaagcactga ctctgatgcc ggtaggttct aaatgggaac tgactatccc gcaggaactg
     2341 gcatatggcg agcgtggcgc aggcgcgtcc atcccaccgt tcagcaccct ggtgtttgaa
     2401 gtcgaactgc tggaaatcct ctaagcagcg cattctgttc ccctcgaacc agaggggagc
     2461 agacattcag caataaaccc ttcagtttgc caaacggcgc tattttgtgt tgcaaagacc
     2521 ccgtaagcgt gtatttttgt gagctgtttc gcgttatcac cgtgatatga cactcacttt
     2581 aaacataaaa ttaacattag atctaaatct tagtattcat cccgcgtatt gttacctaat
     2641 atcgatgagt cccgatacag attcgtcgta tcatagactg actaaaggcc gtagagcctg
     2701 aacaacacag acaggtacag gaagaaaaaa acatggtaga tcaggtaaaa gtcgttgccg
     2761 atgatcaggc tccggctgaa cagtcgctac ggcgcaatct cacaaaccga catattcagc
     2821 ttattgccat tggcggtgcc attggtacgg ggttgtttat ggggtccggc aaaacgatta
     2881 gccttgccgg gccgtcgatc attttcgttt atatgatcat tggttttatg ctctttttcg
     2941 tgatgcgggc aatgggggaa ttgctgcttt cgaatctgga atacaaatct tttagtgact
     3001 tcgcttccga tttactcggg ccgtgggcag gatatttcac cggctggacc tactggttct
     3061 gctgggttgt aaccggtatg gcagacgtgg tggcgatcac ggcttatgct cagttctggt
     3121 tccccgagct ctccgactgg gtcgcctcgc tggcggtgat agtgctgctg ctgacgctca
     3181 acctcgccac cgtgaaaatg ttcggtgaga tggagttctg gtttgcgatg atcaaaatcg
     3241 tcgccatcgt gtcgctgatt gtcgtcggcc tggtcatggt ggcgatgcac tttcagtcac
     3301 cgaccggtgt ggaagcgtca ttcgcgcatt tgtggaatga cggcggctgg ttcccgaaag
     3361 gtttaagtgg cttctttgcc ggattccaga tagcggtttt cgctttcgtg gggattgagt
     3421 tggtaggtac aacagctgcg gaaaccaaag atccagagaa atcactgcca cgcgcgatta
     3481 actccattcc gatccgtatc attatgttct acgtcttcgc gctgattgtg attatgtccg
     3541 tgacgccgtg gagttcggta gtcccggaga aaagcccgtt cgttgaactg ttcgtgttgg
     3601 taggtttgcc tgcggctgcc agcgtgatca actttgtggt gctgacctct gcggcgtctt
     3661 ccgctaacag cggtgtcttc tctaccagcc gtatgctgtt tggtctggcg caggaaggtg
     3721 tggcaccgaa agcgttcgct aaactctcta agcgcgcagt acccgcgaaa gggctgacct
     3781 tctcttgtat ctgtctgctc ggcggcgtgg tgatgttgta tgtgaatccc agcgtgattg
     3841 gcgcgttcac gatgattaca accgtttccg cgattctgtt tatgttcgtc tggacgatta
     3901 tcctttgctc gtacctggtg tatcgcaaac agcgtccgca cctgcatgag aaatcaatct
     3961 acaagatgcc gctcggcaag ctgatgtgct gggtatgtat ggcattcttt gtgttcgtgg
     4021 tcgtgctgct gacactggaa gatgataccc gccaggcgct gctggttacc ccgctgtggt
     4081 ttatcgctct ggggctgggc tggttgttta ttggtaagaa gcgggctgct gaactgcgga
     4141 aataaccgca ttatcatgct ggatggcgca atgccatcca gcttttagat cactcacccg
     4201 ccagggcgcg tgggaacagt acattgtttt ccagactgat gtgatccatc aggtcatcaa
     4261 tcagttcatt aataccgtta tacatcgctt tccaggtggt gcaggcttct ggcggcggtg
     4321 tgacgttatt ggtggtgtgt ttaatcactt ccagcagttc gcccgcttca tcgtgctcgc
     4381 tttccatcac gctgattggt cccattgcct ggctgcccat gccttgtttg atcatcggga
     4441 agagaatttg ctcttctttc atcatgtggc tggaaagctc ttcatgcagc atagtcaggt
     4501 attttgtcag cccttttggc acgctcggtt tgtcggcgtg aacgcgttcg actttgctgg
     4561 cttgcagaat cagctccggc agttgctcgc ggtgacgatc gtggtagcgc acgatgatat
     4621 ggtcgatgat ttctgccagc ggggcgctac gccagtcttt ctcaatcggt tgttcagcga
     4681 gctttgccag ctcagcttca atgacctcaa catccagctc tttacgtgcc gccgcacgcg
     4741 ccagcgtttg cttaccgccg cagcagtaat ccatatcata tttacgaaac agagccgaag
     4801 cgcgaggaat agagagcgcc agttcaccta aaggttggtc gcgataagcc atagctgata
     4861 cctcattctt aataagataa gatgcatttt aaatgcatct ttaaggcaaa agctataacc
     4921 cttacgtagc agaggcttat ttaactcact gccagcgtgt caactttcag agacaggttc
     4981 aattaagggt ttttccggtt ttgctctgac cgcaatgacc acgccaacca ctaacagcgc
     5041 gataccgctc agcgtcatta gtggcggcat ttgctggcgt aacaaaaagg tgtacagcaa
     5101 acctgccagc gtttcgaaga caatcagtgg cccgagaatc accgttggta atcgctggct
     5161 ggcgacgttc cagcaaagtg cgccgaccca tgagcaaagc acagctatcg caatcatcag
     5221 gctaacaaat acaagcggac gggggccaaa aggtagggag aagtccggcg tttgcgtatt
     5281 cagccagtaa caagcgacga gatagccgat aagcgaaacc ggcagcgtga ccagcgcctg
     5341 cgccgtcgcc cacatcatcg gatgtttgtc gggattttcc cgcagccagc gggcgttgcg
     5401 cagggcatac catgcccagc agaccacgga aactaacgcc agcacgatgc cagaggtata
     5461 acgtgcccag tcaaaatcgg ggagtccgtg gtttaactca gcaatattca cactcgccag
     5521 gccgatgcca atacaaatca gtgccggggc gagttttccc cacgcgagtt tgccgtcgcg
     5581 ctggctataa agcagattgg caaagacagg aatgaccacc ggcagggtgc cgataatcat
     5641 cgtggaaaca ggcgcgccag tacgttgaat ggcactggca aggcagaagt aataaatgag
     5701 gttgcccatc atagtgagca tcaaggcggt aagccagtcg cgacgcgcca actgacgcag
     5761 acgcacgcgc cccagccagg caatgggcag cgcaattaac cctaacgcca gataacgccc
     5821 catagactgc aacatcgccg ggtattccgg cacgatcaac ggcccgacaa aaataagccc
     5881 ccacatcaac cctgctaaca gggcgtacag cacgccgcta atcattactg gcatccattg
     5941 gtctgtcaga agaaacgttc aggaagcaga catgattgct cccggcatcc cgtcattatc
     6001 agagaggatg cgggaggatg ccactttaat taactagtta ttaacattaa aaagatggct
     6061 tacgctttcg gctaacgttg tcgttgggcg gccaatcaat ttgctaagcg ttttgctgtt
     6121 atcaaacaga ccacctttcg atgcgccaac gtcagaatcc gccagcatat ccgccagtcc
     6181 gtcgggcagt ccgacgcttt tcagtgccgc ggcgaaatcg gcttcgctca gattttgata
     6241 ggtaacggat tttccgctct gtttggtcag ctctgccgct aactgtgtca acgtccaggc
     6301 actatcgccc gccagttcgt aaaccttgcc ttcgtgaccg gcttcgctaa tcacgcgtgc
     6361 cgcagctgcc gcataatctg cccgcgttgc tgaggcgatt ttgccatcgc ccgccgcacc
     6421 gataaatacg ccgtgttcca gtgctgccgg ggcgctggcg aggtagtttt cggtgtacca
     6481 gccgttgcgc agcagggtgt aaacgatgcc agaatcagcc aacattttct ccgtctcaat
     6541 gtgctcatcg gcgaggccga gcggagattt atccgcgtga agcagactgg tataagcgat
     6601 aaatttcaca ccagccgcct ttgcggcatt aataacatta cgatgctgcg gggcacgttg
     6661 acccacttcg ctggaagaga tcagcagtag tttttccact ccctgaagtg cagatgtcag
     6721 tgcggcttca tcgccgtagt cagcctgacg cacggtaatg ccttgtgctg tcagtgactg
     6781 ggcttttgcc ggattacgaa cgatagccac tatttggctg gcaggaaccg ttttcatcaa
     6841 ggattcaata acatagtgac caagttggcc agtggcacca gtaattgcga tcataggaag
     6901 tctccatcgt tttgtcttgt gtttaaatac gctaacacct aaacttactt ttagtaagta
     6961 cgtacaaaaa ggtaagtatg agatgagtca ggttagcctg tcgcaacaac tgaaagaggg
     7021 aaatctcttc gctgaacagt gtccgtcgcg cgaggttttg aaacacgtca ccagccgttg
     7081 gggggtgttg attctggtgg cgctacgcga aggtactcat cgctttagcg acctgcggcg
     7141 caaaatgggc ggggtgagtg aaaagatgct tgcgcagtcg ttacaggcgc tagagcagga
     7201 tggttttctt aaccgtatcg cgtatccggt ggtgccgccg catgtggaat atagcctcac
     7261 gccgctgggc gagcaggtga gcgaaaaggt tgctgcactg gcggactgga ttgagttgaa
     7321 tttgcccgag gtgttggcgg tgcgggatga acgtgcggcg taactgcagg atgcgcggcc
     7381 tacggtgtat gacttgtagg gcggataaga tgcgccagca tcgcatccgc caataaatgc
     7441 cggatgcggt tgatgatttg caggccgaaa aagaagtgta ttacttactc aaatccacct
     7501 gataaatcgc aaacccgata tcatcggtcg cgactttatt catcggatat tgccctttct
     7561 ctttaataaa cgccgcggct ttatctgatg gagaggtttc gaaacggata tccagtttct
     7621 tatcgccagc tattggtgct aaacgccagt tgttatctgc cgccgggtga attttcccca
     7681 cacgtttcga ctcatcagca atccacgctg ccagcaccga gcggttctca tccggtgaag
     7741 caaaagcgat atggctgtcg cccgtaccgg caaatttgcc gccgtaagcg cgatagttat
     7801 tggtggcaac gaggaacatg gcgttcggat caatcggttt gccattaaag gtcaggttct
     7861 taatcctttc cgcattggca ttaatcatct ggcactcgcc gtcataacgg gcgggttggg
     7921 taacatcaat ctgataattc acaccatcaa taacatcaaa gttataggtg cggaaaccat
     7981 cccagttgat gagtgactgt ggtttcgtgc tgttaggatc aatctggtta aactgtccgg
     8041 cggagcactc cagccactct ttcacctctt taccgctggc tttcaccaca atcagcgtat
     8101 tggggtagag ataaagatcg gcggcattac ggaaggtcaa ctggcctttt tccacctcca
     8161 catagcttgc cgggtcattt ttgcgaccac caactttaaa cggtgcggca gctgaaagca
     8221 ccggcagttt tgccagatcc ggatcgccct gaatgtaatg ctcgacatac gctttttgcg
     8281 cgttgttcac tacctgcacg gtcggatcgt cttgcactag cgccagatag ctatacatat
     8341 tgtcggcgga tttaccgatt ggcttgctga cgaactggcg tgtggcatcg tgatcggctt
     8401 tgagtgtttc taccagcttg ctgtcttccg ccgcgaggga ttttttattc gcgatgtcgt
     8461 aaatcggccg tgcttccgct ttcgcctgcg tcacctgcca tttaccgctg tcattactaa
     8521 gttgtaagtc gaccacacca agatgatcgc cccacatgcc tggcattacc gccggaacac
     8581 cattcagcgt gcctttcgcg atatcagccc cttcgatatc agcaaaatct ttacctggga
     8641 aaacggcgtg agcatggcca aacataatgg cgttaacgcc cggaatttca ctgaggtaat
     8701 aaactgagtt ttctgccatc actttatacg gatcggcaga cagaccagaa tgcgccagaa
     8761 cgacaacgac atcggcacct ttctcgcgca tttcaggcac gtacttgcgc acggtttcgg
     8821 taatatcatt caccgtcact ttcccggata aattagcttt atcccagccc atgatttgcg
     8881 gcggcacgac gccaatatag ccaatcttca gcgtctgttt ttttccgtct ttatcgacca
     8941 cttcggtgtc tttaattaaa tacggtgtaa acattggctg tttggttctg gcgtcaatga
     9001 cgttggcatt tacataaggg aatttcgctc ccgccagcgc atttttcagg taatccagac
     9061 cgtagttaaa ctcgtggttg ccaagcgtgc cgacggtata gtccagcgta tttaatgcct
     9121 tatagaccgg gtgaatatca cctgctttta aacctttcgc cgacatgtaa tcggccagcg
     9181 gactcccctg aatcaaatcg ccgttatcga ccagtacgct gtttttcact tcattgcggg
     9241 catcgttaat caggcttgcg gtacgtacca gtccgaattt ttccgtggcg gtgtctttgt
     9301 aataatcgaa atccatcatg ttgctatgca gatcagtggt ttccatgata cgcagatcga
     9361 ccgtcgctgc attcacactg gcggcaatca gcgtggccag gagcgttgcg ctaaacttaa
     9421 tcatcaggga catcctttta tcatcgggaa tacgaaagaa aagggagaat aaacgtctta
     9481 cttatagaac agtgaagaat gccacaattt tacgctttga aaatgatgac actatcacag
     9541 ttggcgcatt cattaacgat agggtataag taaaacaata agttaacacc gctcacagag
     9601 acgaggtgga gaaatgttag atcaagtatg ccagcttgca cggaatgcag gcgatgccat
     9661 tatgcaggtc tacgacggga cgaaaccgat ggacgtcgtc agcaaagcgg acaattctcc
     9721 ggtaacggca gcggatattg ccgcacacac cgttatcatg gacggtttac gtacgctgac
     9781 accggatatt ccggtccttt ctgaagaaga tcctcccggt tgggaagtcc gtcagcactg
     9841 gcagcgttac tggctggtag atccgctgga tggtactaaa gagtttatta aacgtaatgg
     9901 cgaatttacc gttaacattg cgctcatcga ccacggcaaa ccgattttag gtgtggtgta
     9961 tgcgccggta atgaacgtaa tgtacagcgc ggcagaaggc aaagcgtgga aagaagagtg
    10021 cggcgtgcgc aagcagattc aggtccgtga tgcgcgcccg ccgctggtgg ttatcagccg
    10081 ttcccatgca gatgcggagc tgaaagagta tctgcaacag cttggcgaac atcagaccac
    10141 gtccatcggc tcttcgctga aattctgcct ggtggcggaa gggcaggcgc agctgtaccc
    10201 gcgcttcgga ccaacgaata tttgggacac cgccgctgga catgctgtgg ccgcagctgc
    10261 cggagcgcac gttcacgact ggcagggtaa accgctggat tacactccgc gcgagtcgtt
    10321 cctgaatccg gcgttcagag tgtctattta ctaaattcag atggcagaaa cagtgtattt
    10381 cctgattctg ccatcctgat ttctcccaac ctaaaaagtt ataaataaaa agagattgta
    10441 tttaaattgc aaaaattcaa ttgctaataa gttatatttt aataatga
//
LOCUS       DAOTRK010000154        10357 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.155, whole genome shotgun sequence.
ACCESSION   DAOTRK010000154 DAOTRK010000000
VERSION     DAOTRK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.155"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            349..1326
                     /gene="csiD"
                     /locus_tag="QIE41_003937"
     CDS             349..1326
                     /gene="csiD"
                     /locus_tag="QIE41_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDS3532762.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1346..2614
                     /gene="lhgO"
                     /locus_tag="QIE41_003938"
     CDS             1346..2614
                     /gene="lhgO"
                     /locus_tag="QIE41_003938"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDS3532763.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2637..4085
                     /gene="gabD"
                     /locus_tag="QIE41_003939"
     CDS             2637..4085
                     /gene="gabD"
                     /locus_tag="QIE41_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDS3532764.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4099..5379
                     /gene="gabT"
                     /locus_tag="QIE41_003940"
     CDS             4099..5379
                     /gene="gabT"
                     /locus_tag="QIE41_003940"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDS3532765.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREFL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5617..7017
                     /gene="gabP"
                     /locus_tag="QIE41_003941"
     CDS             5617..7017
                     /gene="gabP"
                     /locus_tag="QIE41_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDS3532766.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7038..7700
                     /gene="csiR"
                     /locus_tag="QIE41_003942"
     CDS             7038..7700
                     /gene="csiR"
                     /locus_tag="QIE41_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDS3532767.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7701..8150)
                     /gene="kbp"
                     /locus_tag="QIE41_003943"
     CDS             complement(7701..8150)
                     /gene="kbp"
                     /locus_tag="QIE41_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDS3532768.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8234..8392)
                     /locus_tag="QIE41_003944"
     CDS             complement(8234..8392)
                     /locus_tag="QIE41_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDS3532769.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8575..8874
                     /locus_tag="QIE41_003945"
     CDS             8575..8874
                     /locus_tag="QIE41_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3532770.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8884..9408
                     /gene="ygaP"
                     /locus_tag="QIE41_003946"
     CDS             8884..9408
                     /gene="ygaP"
                     /locus_tag="QIE41_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDS3532771.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9455..9859)
                     /gene="stpA"
                     /locus_tag="QIE41_003947"
     CDS             complement(9455..9859)
                     /gene="stpA"
                     /locus_tag="QIE41_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDS3532772.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2419 a         2635 c         2779 g         2524 t
ORIGIN      
        1 ctctatttgt aatttaatgt tatatctgcc ccgctaaaac ggggcagata atatgtttag
       61 tttactaacg gtcattttgc agtgaagcca tttaccgttt tttatcgacc agataatctg
      121 ttctctgatg gtaatcctcc cctaacctgt tgctttagtt attcatttcc tgtctcactt
      181 tgccttaata ccccacgtta aatgttacca atttgttgct tttgatcaca ataagaaaac
      241 aatatggcgc atttgtgcgc atttttcaga aatgtagata tttttagatt atggctacga
      301 aatgagcatc gccatgtcac cctacatctc ataagaggat cgcttctgat gaatgcactg
      361 accgccgtac aaaataacgc tgtcgattca ggccaggact atagcggatt cactctcatc
      421 ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca ccgaacagac gaccaaacag
      481 tttctcgaac aggttgccga atggcccgtg caggcgctgg agtacaaatc ttttctgcgt
      541 tttcgggtag gcaaaattct tgacgatctg tgtgcgaacc agctgcaacc attgctgttg
      601 aagaccctgc taaaccgtgc tgaaggtgcg ctgttgatca atgcggtggg tgtcgatgat
      661 gtgaagcaag cggatgagat ggtgaagctg gcaacggcgg tagcgcatct gattggtcgc
      721 tcaaatttcg atgcgatgag cggtcagtat tacgcgcgat tcgtggtgaa aaatgtcgat
      781 aactcagaca gctatctgcg tcagccgcac cgcgtaatgg agctgcacaa cgacggcacc
      841 tacgtcgaag agatcaccga ctatgtgctg atgatgaaaa tcgacgagca gaacatgcag
      901 ggtggaaatt cgttgctgct gcatctcgat gactgggaac atctggacca ctatttccgc
      961 cacccgctgg cgcgtcgccc gatgcgcttt gccgcgcctc cgagcaaaaa cgtcagcaaa
     1021 gatgttttcc atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgttatatc
     1081 gaccagttcg tccagccaaa agacttcgaa gaaggcgtgt ggctgagcga gctttccgat
     1141 gccattgaaa ccagcaaagg cattctttct gtgcccgttc ccgttggcaa attcctgttg
     1201 attaacaatc tattctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc
     1261 cgtgaactga tgcgtcagcg tggctatttc gcttacgcct ctaaccacta ccagacgcat
     1321 cagtaagcgc aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat
     1381 cggcatgtcg accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct
     1441 ggaaaaagag tccggtccgg cctgtcatca gacaggccac aacagcggcg tgatccatgc
     1501 cggggtctat tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg gaaaccgcgc
     1561 caccaaagcc ttttgcgatc aaaacggcat tcgctatgac aactgcggca agatgctggt
     1621 cgccacctct gaacttgaaa tggaacggat gcgcgcgttg tgggaacgca cggcggcgaa
     1681 cggtatcgag cgcgagtggt taaacgccga ggaactgcgc gagcgcgaac cgaatatcac
     1741 cgggctcggc ggcatttttg tgccgtccag cggcattgtc agctatcgcg aagtgacggc
     1801 ggcgatggca aaaatcttcc aggccagagg cggcgagatt atttataacg ccgaagtcag
     1861 cgccctcagt gagcataaaa acggcgtggt gatacgtacc cgtcagggcg gtgaatatga
     1921 agcatcgacg ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg
     1981 cctcgaaccg ggctttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga
     2041 gcataaccag attgttaacc acctgattta ccccattccc gaccccgcga tgccattttt
     2101 gggcgtgcat ctcacccgca tgatcgatgg cagcgtgacc gtcgggccaa acgcggtgct
     2161 ggcttttaaa cgcgaaggct atcgcaagcg cgatttctcg tttagtgaca cgctggagat
     2221 tttaggctcg tcggggattc gccgggtgct gcaaaaccat ctacgctcag gattgggcga
     2281 gatgaaaaac tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg
     2341 gctttcgtta agcgatctcc agccctggcc agcaggtgtg cgggcgcagg cggtatcgcc
     2401 ggacggcaag ctgattgacg attttctgtt tgtcaccacc ccgcgcacga tccacacctg
     2461 caatgcgccc tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa
     2521 ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag
     2581 tgtggatgcc ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga
     2641 aacttaacga cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg
     2701 ccaacaatgg cgaggtcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg
     2761 tacccaaaat gggcgctgat gaaacccgcg ccgctatcga cgccgccaac cgtgctctgc
     2821 ccgcctggcg tgcgctcacc gccaaagaac gcgccaccat tctgcgcaac tggttcaatt
     2881 tgatgatgga gcatcaggac gatttagcgc gcctgatgac cctagaacag ggcaaaccgc
     2941 tggctgaagc gaaaggcgaa attagctacg ccgcctcctt tattgagtgg tttgccgaag
     3001 aaggcaaacg catttatggc gacaccattc ctggtcatca ggccgataaa cgcctgattg
     3061 ttatcaagca gccgattggc gtcaccgcgg ctatcacgcc gtggaacttc ccggcggcga
     3121 tgattacccg caaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg
     3181 ccagtcagac gccgttctct gcgctggcgc tggcggagct ggcgattcgc gcaggcgttc
     3241 cggctggcgt atttaacgtg gtcaccggtt cggcgggcgc ggtcggtaac gaactgacca
     3301 gcaatccgct ggtgcgcaag ctgtcgttta ccggctcgac cgaaattggc cgccagttaa
     3361 tggaacagtg cgcgaaagac atcaagaaag tgtcgctgga gctgggcggc aacgcgccgt
     3421 ttatcgtctt tgacgatgcc gacctcgaca aagccgtgga aggcgcactg gcctcgaaat
     3481 tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt
     3541 atgaccgctt tgccgaaaaa ttgcagcagg cggtgagcaa actgcacatc ggcgacgggc
     3601 tggagaaagg cgtcactatt gggccgctga ttgatgaaaa agcggtagca aaagtggaag
     3661 agcatatcgc cgatgccctg gagaaaggcg cgcgcgtggt ttgcggcggt aaagcacatg
     3721 aacgcggcgg caacttcttc cagccgacca ttctggtaga cgttccggcc aacgccaaag
     3781 tgtcgaaaga agagacgttc ggccccctcg ccccgctgtt ccgttttaaa gatgaagccg
     3841 atgtgattgc acaagccaat gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg
     3901 atttaagccg cgtcttccgc gtgggcgagg cgctggaata cggcatcgtc ggcatcaata
     3961 ccgggattat ttccaatgaa gtggccccgt tcggcggcat taaagcctcg ggtctgggtc
     4021 gtgaaggttc gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc
     4081 tttaactgga gaacgcgaat gagcagcaat aaagagttaa tgcagcgccg tagtcaggca
     4141 gttcctcgtg gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg
     4201 gtgtgggacg ttgaaggccg tgagtttctt gatttcgcgg gcgggattgc ggtgctcaat
     4261 accgggcacc tgcatccgaa agtggttgcg gcggtggaag cgcagttgaa aaagctgtcg
     4321 cacacctgct tccaggtgct ggcttacgaa ccgtatctgg agctgtgcga gattatgaat
     4381 cagaaggtgc cgggagattt cgccaaaaaa acgctgctgg taacgaccgg ttctgaagcg
     4441 gtggagaacg cggtgaaaat cgcccgcgcc gccaccaaac gcagcgggac catcgccttt
     4501 agcggtgcat atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg
     4561 tactctgcgg gcatgggact gatgcctggg cacgtttatc gcgcgcttta tccttgcccg
     4621 ctgcacggca tcagtgaaga tgacgctatc gccagcattc accgaatttt taaaaatgac
     4681 gctgcaccgg aagatatcgc cgccatcgtg attgagccgg ttcagggcga aggcggtttc
     4741 tacgccgcga cgccagcctt tatgcagcgt ttacgcgcgc tgtgtgacga gcacgggatc
     4801 atgctgattg ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcgatg
     4861 gagcagatgg gcgttgcgcc ggatcttacc acctttgcga aatcgatcgc gggcggcttc
     4921 ccgctggcgg gcgtcaccgg gcgcgcggaa gtgatggatg ccgtcgctcc aggcggtctg
     4981 ggcggcacct atgccggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg
     5041 tttgagcagg aaaatctgct gcaaaaagcc aacgatctgg ggcagaagtt gaaagacgga
     5101 ttgctggcga tagccgaaaa acacccggag atcggcgacg tacgcgggct gggagcgatg
     5161 attgccatcg agctgtttga agatggcgat cacagcaaac cggacgccaa actcaccgcc
     5221 gagatcgtgg cgcgtgcccg cgataaaggc ctgattcttc tctcctgcgg cccgtattac
     5281 aacgtgctgc gcatccttgt accgctcacc attgaagacg ctcagatccg tcagggtctg
     5341 gaaatcatca gccagtgttt tgctgaggcg aagcagtaga tccgtaccca tgccggaggc
     5401 gacgctgcgc gtcttgtccg gcctacgcga atccaggtcg gataaagcgt ttacgctgca
     5461 tccgacaatc tgtacgtgaa caggaataaa tctatgtcgg ccggataagg cggagccgct
     5521 ctccggtaaa aagaaccaat aacaattaca cgcgtgaccc gacgcgggaa atgtcggggc
     5581 gctctcccaa gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacat
     5641 gagttaggcg gcgggctgaa gtcacgccac gtcaccatgt tgtctattgc cggtgttatc
     5701 ggcgcaagtc tgtttgtcgg ttccagcgtc gctatcgccg aagcgggccc ggcggtatta
     5761 ctggcctatc tgttcgccgg attactggtg gttatgatta tgcggatgtt ggcggaaatg
     5821 gcggttgcca cgcccgacac gggttcgttt tccacctatg cagataaagc tattggtcgt
     5881 tgggcgggct ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccgctg
     5941 gaagccaaca tcgccgccat gatcctgcac tcgtgggttc caggcattcc catctggtta
     6001 ttttccctcg tcattaccct cgccttaact ggcagtaatt tattaagcgt taaaaactac
     6061 ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc
     6121 cttggtgcag tcgcaattag cggtttttac ccttatgccg aagtgagcgg gatctcaaga
     6181 ttgtgggata gcggcggctt tatgcccaac ggcttcggcg cggtattaag cgcgatgttg
     6241 atcaccatgt tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatctgac
     6301 acgccggaaa aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc
     6361 tatttgtgct ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa
     6421 gccgttggat cttatcgctc ggttctggag ttgctcaata ttccccatgc gaaattaatc
     6481 atggactgcg tgatattact ttccgtaacc agttgcctga actcggcgct gtataccgct
     6541 tccagaatgc tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc
     6601 aaccgcagta aaaccccgta tgtggcggta ttactctcga ccggtgcggc atttttaacg
     6661 gtggtggtga attattacgc gcctgcgaaa gtgtttaaat ttctgatcga cagctccggc
     6721 gctatcgccc tgctggttta tttagtcatc gccgtttcac aattgcggat gcgcaaaatt
     6781 ctgcgagcag aaggaagcga aattcgcctg cggatgtggc tttatccgtg gctcacctgg
     6841 ctggtcatcg gctttattac ctttgtgttg gtagtgatgc tattccgccc ggcgcaacag
     6901 ttagaagtga tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg
     6961 gctcgctgga aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgataa
     7021 tgatcaggag tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg
     7081 atattattcg cggtaatttt caaccggatg aaaaattacg gatgagtttg cttacatcgc
     7141 gttatgcact tggcgttggg ccgttacggg aagctctttc gcaactggtg gcggaacggc
     7201 tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc
     7261 tcgatatttt cgacgcccgc gccaatatgg aagcgatgct ggtcagtctg gcgattgccc
     7321 gcggtggcga tgaatgggag gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg
     7381 aggcctgtga cgccagtgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc
     7441 atacggcaat tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt
     7501 ttgatctggc ggcgcgctat cgatttatct ggctgcggcg aacagtgctt tcggtggaga
     7561 tgctggagga taaacacgat cagcaccaga ccctgactgc ggcggtactg gcgcgagata
     7621 ccgcgcgcgc cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc
     7681 aggcgatggc tggcaattaa ctactcttca ggaatacgca acacttgccc cgggtaaatt
     7741 ttatccgggc ttttcagcat cggtttattc gcttcgaaga ttttattgta cagattggcg
     7801 ttaccgtaga cctgtttaga aatggcgcta agagtgtcgc cagacttaac ggtataaaac
     7861 tggctggcag cggctggtgt cgccgttttc acctgatcat cgacactggc aataccggaa
     7921 atattcccca ccgcaacaag gattttctcc ttcgcctcct gacttaagcc gtcacccgtg
     7981 accgtcgctt tgccgtcggc gatttgaatg ttcactttat cggcatcagg tataccggtt
     8041 ttgttcagat gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc
     8101 gcgtcccaga gtttttctcc ggcatctttc acaaaattga acagacccat agctacctca
     8161 ttcgttaacg gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg
     8221 ggtgaacaca ggtttaatcg cgggtttgca cccagaaagc gtgaatcaaa ccggggatat
     8281 acccaagcag tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca
     8341 gcacgccgag cggcggcaga atgatggtga tgacgattct ccagaaaccc atatgtactc
     8401 cctataagaa aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt
     8461 agcggagcct gccagtttca gcttacgctt ttactctcat ttacgctgct gatgctggca
     8521 tatgttatat ttttagttta gagtttactt aatttagaaa atgcttaaat aactatgact
     8581 gaactcgcgc aattacaggc cagtgccgaa caggcagcgg ccttattgaa agcaatgagc
     8641 caccctaaac ggttgctgat tctgtgcatg cttagcggtt ctcccggcac cagtgcggga
     8701 gagctgacgc gcattaccgg actaagtgcc tctgcaacat cacagcatct cgctcgtatg
     8761 cgggacgaag ggcttatcga cagccaacgg gatgcccaac gcattctata ttccattaaa
     8821 aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg tctattgtcc gtaaggagtc
     8881 actatggctt tgacaaccat ttcgccgcat gatgcacaag aattaatcgc acgcggcgca
     8941 aagttaatcg atattcgtga cgctgatgaa tatcttcgtg agcatattcc tgaagcagat
     9001 ctggctccat tatccgtgct ggaacagtca ggtcttccgg ctaaattacg tcacgagcaa
     9061 attatattcc actgccaggc aggtaaacgc accagtaata acgccgataa attagcagcg
     9121 attgccgccc ccgcagaaat ctttttactc gaagatggga ttgatggctg gaaaaaagca
     9181 ggattaccag tagcggtaaa taaatctcaa cccttgccgt taatgcgcca ggtgcagatc
     9241 gctgcgggtg gtttaatatt aatcggcgtt atactcggtt ataccgtaaa tagcggtttc
     9301 ttcttattaa gcggctttgt tggtgccggg ttactgtttg caggaattag cggtttttgt
     9361 gggatggcaa ggttgttaga taagatgccg tggaaccaac gagcttgaga agcgacgccg
     9421 gacgcgccat agcggcggca tccggcctca gtaattagat caggaaatcg tcgagagatt
     9481 taccttctgc cagcgcctga gcgattggct tcggtgtacg gccctgaccg gtccaggttt
     9541 tagtttcacc gttaacatcg gtgaatttat atttcgccgg acgcggctgg cgttttttac
     9601 cagcgcgtgg ggcggccgcg gagctattac ccaataactc ttccgggtta attccgtcag
     9661 ctttcatcag ctccagccag gtgctaattt tttcctggcg ctctgccagt tcacgctgct
     9721 gctgttcttc ttcttcacgt ctttctttag tgacaaccct gaatttttcg agcatttctt
     9781 caagaacgtc aatggagaat tcgcgagcca tcgcacggag ggtgcgaatg ttatttaaac
     9841 tttgtaacat tacggacata ataaagaaaa ccttttaacg ccaaaacaaa aaagtatttc
     9901 gtcgcgtagt gtaagcgatc acttattata tttccacaaa atttaatcta tttacagtcc
     9961 tgcgcgagat tatttcagct gttattttct gaaactctca gaataatttc aagattatat
    10021 attagccatt ttgatgagta atttcggtta tttgtgagca gagcggcgct ctctgctcgc
    10081 taattccgca agcctgacaa aacaaaaatg aataaaaatg caatttaacc gaataaatgg
    10141 aaacacccct cactggtgct ttctgctgtc aatgtacatt ttctggcata aaaaatgctg
    10201 aaaatgaccc ggtcacgaca tacaccgaat tctctacgct tatagcgtta cctcacccct
    10261 aaaccttcat gagtgataat aaaggtaaac acatatccaa ttgatttaat taaaaataaa
    10321 tcatatcggc atgtaaaaat ccacaatgta caaaaaa
//
LOCUS       DAOTRK010000155        10320 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.156, whole genome shotgun sequence.
ACCESSION   DAOTRK010000155 DAOTRK010000000
VERSION     DAOTRK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.156"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            16..897
                     /locus_tag="QIE41_003948"
     CDS             16..897
                     /locus_tag="QIE41_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3532773.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAIEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1102..2571
                     /gene="lysP"
                     /locus_tag="QIE41_003949"
     CDS             1102..2571
                     /gene="lysP"
                     /locus_tag="QIE41_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDS3532774.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2864..4843
                     /gene="cirA"
                     /locus_tag="QIE41_003950"
     CDS             2864..4843
                     /gene="cirA"
                     /locus_tag="QIE41_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDS3532775.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEDGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4875..5711)
                     /gene="yeiG"
                     /locus_tag="QIE41_003951"
     CDS             complement(4875..5711)
                     /gene="yeiG"
                     /locus_tag="QIE41_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDS3532776.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5969..6637
                     /gene="folE"
                     /locus_tag="QIE41_003952"
     CDS             5969..6637
                     /gene="folE"
                     /locus_tag="QIE41_003952"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDS3532777.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6654..7811
                     /locus_tag="QIE41_003953"
     CDS             6654..7811
                     /locus_tag="QIE41_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDS3532778.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLMLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            7953..8993
                     /gene="galS"
                     /locus_tag="QIE41_003954"
     CDS             7953..8993
                     /gene="galS"
                     /locus_tag="QIE41_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDS3532779.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9273..10271
                     /gene="mglB"
                     /locus_tag="QIE41_003955"
     CDS             9273..10271
                     /gene="mglB"
                     /locus_tag="QIE41_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDS3532780.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
BASE COUNT         2432 a         2552 c         2789 g         2547 t
ORIGIN      
        1 aagtggtaag cgactatgca catcaccctc cggcagttgg aagtttttgc agaagtattg
       61 aaaagtggat caaccaccca ggcgtcggtg atgctggcgt tgtcgcaatc agcagtgagc
      121 gcagccttga ccgacctgga agggcagctt ggcgttcaac tgtttgatcg cgtggggaaa
      181 agactggtag ttaatgaaca cgggcgactg ctctatccgc gcgcgttggc actgcttgaa
      241 caggcgatcg aaatcgaaca actgtttcgc gaagacaacg gtgcgattcg tatctatgcc
      301 agtagtacca tcggtaacta cattctgcct gcagttatcg cccgttatcg ccatgattat
      361 ccgcagttgc cgattgaact tagcgttggg aacagccagg acgtgatgca agcggtgctg
      421 gatttccgcg ttgatattgg ctttattgaa ggaccgtgcc acagcactga aatcatctca
      481 gaaccgtggc tggaagacga gctggtggtt ttcgccgcgc cgacttcacc attggctcgt
      541 ggacctgtca ccttagaaca gctggccgct gcgccgtgga tcctgcgtga acgcggctcc
      601 ggcacgcggg agattgtcga ttatctgttg ctgtcacatt taccgaagtt tgagatggcg
      661 atggaattag gtaactccga ggcaatcaaa catgcggtgc gtcatgggtt gggaattagt
      721 tgcctgtcgc ggcgggtgat tgaagatcaa ttgcaggcag gcacattaag tgaagttgcg
      781 gtccctctgc cgcgcctgat gcgtacgttg tggcgtatac atcatcggca aaaacacctt
      841 tccaacgcgc tacggcgctt tctggactat tgcgatcccg cgaatgtacc gcgttaagtt
      901 gctgtacaag aacatgctgg tgctgtgtcg atttcgtgac gcagcgccct cagcatgcat
      961 tcgccagaaa agagattggc tgctttactt ataatccctg ggcgatcatg aaggtgtctt
     1021 ataaccgtgt atttctgccg gaaggattgc caatcgtctg ctacaatcgc gcctcatttt
     1081 taagatggat agcatttttg tatgggttcc gaaactaaaa ctacagaagc gccgggctta
     1141 cgccgtgaat taaaggcgcg tcacctgacg atgattgcca ttggcggttc catcggtaca
     1201 ggtctttttg ttgcctctgg cgcaacgatt tctcaggcag gtccgggcgg ggcattgctc
     1261 tcgtatatgt tgattggcct gatggtttac ttcctgatga ccagtctcgg tgaactggct
     1321 gcatatatgc cggtttccgg ttcgtttgcc acttatggtc agaactatgt tgaagaaggc
     1381 tttggcttcg cgctgggctg gaactactgg tacaactggg cggtgactat cgccgttgac
     1441 ctggttgcag ctcagctggt catgagctgg tggttcccgg atacaccggg ctggatctgg
     1501 agtgcgctgt tcctcggcgt tatcttcctg ctgaactaca tctcagttcg cggctttggt
     1561 gaagcggaat actggttctc actgatcaaa gtcacgacag ttattgtctt tatcatcgtt
     1621 ggcgtgctga tgattatcgg tatcttcaaa ggcgcgcaac ctgcgggctg gagcaactgg
     1681 acaatcggcg aagcgccgtt tgctggtggt tttgcggcga tgatcggcgt agctatgatt
     1741 gtcggcttct ctttccaggg aaccgagctg atcggtattg ctgcaggcga gtccgaagat
     1801 ccggcgaaaa acattccacg cgcggtacgt caggtgttct ggcgaatcct gttgttctat
     1861 gtgttcgcga tcctgattat cagcctgatt attccgtaca ccgatccgag cctgctgcgt
     1921 aacgatgtta aagacatcag cgtcagtccg ttcaccctgg tattccagca cgcgggtctg
     1981 ctctctgcgg cggcggtgat gaacgcagtt attctgacgg cggtgctgtc agcgggtaac
     2041 tccggtatgt atgcgtctac tcgtatgctg tacaccttgg cgtgtgacgg taaagcgccg
     2101 cgcattttcg ctaaactgtc gcgtggtggc gtgccgcgta atgcgctgta tgcgacgacg
     2161 gtgattgccg gtctgtgctt cctgacctcc atgtttggca accagacggt atacctgtgg
     2221 ctgctgaaca cctccgggat gaccggtttt atcgcctggc tggggattgc cattagccac
     2281 tatcgcttcc gtcgcggtta cgtattgcag ggacacgaca ttaacgatct gccgtaccgt
     2341 tcaggtttct tcccattggg gccgatcttc gcattcattc tgtgtctgat tatcactttg
     2401 ggccagaact acgaagcgtt ccttaaagat accattgact ggggcggcgt agcggcaacg
     2461 tatattggta tcccgctgtt cctgattatt tggttcggct acaagctgat taaaggaact
     2521 cacttcgtac gttacagcga aatgaagttc ccgcagaacg ataagaaata agtttcctcc
     2581 cttccttgct aagccctctc aaccgagagg gctttttcaa ttccatttcc ctgacaaatc
     2641 atgcggatat aaaatttaac atttggattg ataattgtta tcgtttgcat tatcgttacg
     2701 ccgcaatcaa aaaaggctga caaatcagag gctgttccgg ctttctggga tgatcacctg
     2761 catacaaata agtccaccgc gatgctgccg tacgcaaggg gacatgaaga agatgtgagc
     2821 gataacccat tttattttcg tagttacctc atggagatat ggaatgttta ggttgaaccc
     2881 tttcgtacgg gtcgggctgt gtttgtccgc tatttcttgt gcatggcctg tgttagcggt
     2941 cgatgatgat ggcgaaacga tggttgtcac tgcatcttcc gttgagcaaa accttaaaga
     3001 tgcacccgcc agtatcagcg tcattaccca ggaagacctg cagcgaaaac cggtacagaa
     3061 tctgaaggat gtcctcaaag aagtgcctgg cgtacaactg acgaacgaag gggataaccg
     3121 taagggcgtt agtattcgtg gtctggacag cagctacacc ctgattctcg tcgacggtaa
     3181 acgcgttaac tcccgcaatg ccgtcttccg ccacaatgat ttcgatctga actggatccc
     3241 ggtcgattcc atcgaacgta ttgaagtggt ccgtggcccg atgtcgtcgc tgtacggttc
     3301 cgatgcgctc ggcggtgtag tgaatatcat caccaaaaaa atcggtcaga aatggtcggg
     3361 taccgttacc gtcgatacca ccattcagga acatcgcgat cgcggtgata cctataacgg
     3421 tcagttcttt accagcggac cattaattga cggcgtgctg ggaatgaaag cttacggcag
     3481 cctggcaaaa cgtgaaaagg atgacccgca aaactcaacg accaccgata ccggagaaac
     3541 gccgcgtatt gaaggattct ccagccgcga cggcaatgtg gaatttgcct ggacaccgaa
     3601 tcaaaatcac gattttactg ccggatacgg tttcgaccgt caggatcgtg attccgactc
     3661 gctggacaaa aaccgcctgg aacgccagaa ctactccgtc agccataatg ggcgttggga
     3721 ttacggcacc agcgaactga aatactacgg tgagaaagtc gagaacaaaa accctggcaa
     3781 cagcagcccg ataacttccg aaagcaatac ggtcgacggc aaatacacgt tgccgctgac
     3841 ggcgattaat cagtttctca cggttggcgg tgaatggcgt cacgacaaac ttagcgatgc
     3901 ggtgaacctg accgggggaa ccagttccaa aacgtctgcc agccagtacg cgctgtttgt
     3961 ggaagatgaa tggcggatct tcgagccgct ggcgctgacg accggcgtac gtatggacga
     4021 tcatgaaacc tacggtgaac actggagtcc gcgtgcctac ctggtttata acgccaccga
     4081 caccgtaacg gtgaaagggg gctgggcgac ggcatttaaa gcaccttctc tgttgcaact
     4141 tagccctgac tggacgagca attcctgccg tggcgcatgt aagattgtgg gtagcccgga
     4201 tctgaaacca gaaaccagcg aaagttggga actggggctt tactacatgg gcgaagacgg
     4261 ctggctggaa ggggtagagt ccagcgttac cgttttccgt aacgatgtga aagatcgcat
     4321 cagcatcagc cgtacgtctg acgtcaatgc tgcaccgggc taccaaaact ttgttggttt
     4381 tacagcgaac ggtgtgccgg tatttagcta ctacaacgtt aacaaagctc gtattcaggg
     4441 cgtggaaacc gaactgaaaa ttccgttcaa cgatgaatgg aaactgtcga tcaactacac
     4501 ctacaacgat ggtcgtgatg tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt
     4561 ccatactgct aacggtacgc tggactggaa accgctggcg ctggaagact ggtcattcta
     4621 tgtttctggt cactataccg ggcagaaacg agccgacagc gcgacggcta aaacaccggg
     4681 cggttatacc atctggaata ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg
     4741 cgcaggcgtg ctgaaccttg gcgacaagga tctcagtcgt gacgactaca gctataacga
     4801 agacggacgt cgttacttta tggcagtgga ttatcgcttc tgatgagaag atgcccggcg
     4861 aaccgggcgg actttcactt cagtaaatat tgcgcatgga agcgcaaatg atcctctata
     4921 aaagaggcga tgaagtagta actgtgatca tatcccggct gaatacgcag cgtcatcggc
     4981 cacgctttct ggcgcgcggc ttctgccagc actgcgggtt gcagctggtc ggcaagaaac
     5041 tgatcattat caccctgatc gataagcgtc gggatcgcat cctgcgcgtt actggcatac
     5101 atcagcgcgc aactgtccca ttccagccat gcatttttgt cctcacctaa ataggtgcta
     5161 aacgctttga ttccccacgg gacgctgcac ggattcacaa ttggcgcaaa ggccgaaacg
     5221 ctagtgtatt tacccggatt tttcagcgcc ataattagcg caccgtgacc acccattgaa
     5281 tgaccgctaa tagcgcagcg gtcgctgaca ttaaattgcg actgaatcag cgccggtaat
     5341 tcatcgcgca gataatcata catccggtaa tgcgtcgccc aaggcggttg cgtggcatta
     5401 agataaaagc ctgcgccctg gcctaaatcg tagccatcgt cgttggcaac ctgttcgccg
     5461 cgcgggctgg tgtctggcat taccagtaca atccccagtt ctgccgccac gcgctgggca
     5521 cccgccttgg tggtgaagtt ctcgtcattg caggttaatc cggaaagcca gtacagcact
     5581 ggaggcgggg tgtgatcacg aggtggtggg agaaagatac tgaacgtcat cgggcagttt
     5641 aaggtactgg agtcgtgtcg ccagcgttgc tgccagcctt caaaacagcg gtgctcttcg
     5701 agcatttcca tgcgtggctc cttgttgtgt tgtttgcaat ttcttcatca taccgtttat
     5761 tcaatgtgct gtgagtaact ttcacttccg tatttgcata acgatgtttt aacatctgct
     5821 gatgaaaggc agcggcaatt acaataatta tcgctgtgaa tactggatta tgtgcgccgc
     5881 ctcacgcaca ataatcaggc tgtaaatcag cttaataact ttgcccccac gcagggcgga
     5941 ggcgtcacac ctgcaggaga aatcataaat gccatcactc agtaaagaag cggccctggt
     6001 tcatgaagcg ttagttgcgc gaggactgga aacaccgctg cgcccgcccg tgcatgaaat
     6061 ggataacgaa acgcgcaaaa gccttattgc tggtcatatg accgaaatca tgcagctgct
     6121 gaatctcgac ctggctgatg acagtttgat ggaaacgccg catcgcatcg ctaaaatgta
     6181 tgtcgatgaa attttctccg gtctggatta cgccaatttc ccgaaaatca ccctcattga
     6241 aaacaaaatg aaggtcgatg aaatggtcac cgtgcgcgat atcaccctga ccagcacctg
     6301 tgaacaccat tttgttacca ttgatggcaa agcgacggtg gcttatatcc cgaaagattc
     6361 ggtgatcggt ctgtcaaaaa ttaaccgtat tgtgcagttc tttgcccagc gtccgcaggt
     6421 gcaggaacgt ctgacccagc aaattctcat tgcgctacaa acgctgctgg gcaccaataa
     6481 cgtggctgtc tctatcgacg cggtgcatta ctgcgtgaaa gcgcgtggca tccgcgacgc
     6541 aaccagtgcc acaacaacga cctctcttgg tggattgttc aaatccagtc agaatacgcg
     6601 ccacgaattt ctgcgcgctg tgcgtcatca caactgatta aaaggcagga accatggagc
     6661 gcaacgtcac gctcgatttt gttcgcggcg tcgccattct ggggatcctg ctattaaaca
     6721 tcagcgcctt tgggctacca aaggcggctt atctcaatcc cgcctggtac ggcgctatta
     6781 cgccgcagga tgcatggacc tgggcatttc tcgatctcat cggccaggtg aaattcctca
     6841 cgctttttgc gctgctgttt ggtgctggcc tgcaaatgtt gctgccccgt ggcagacgct
     6901 ggatccagtc gcggttaacg ctgttagtct tgctgggctt tattcacggt ttattgttct
     6961 gggacggcga tattctgctg gcttacgggc tggtgggctt aatctgctgg cggctggtgc
     7021 gcgatgcgcc atcagtaaaa agcctgttta atacaggcgt catgctttat ctggtggggc
     7081 ttggcgtttt gatgttattg gggctgattt ccgacagcca gaccagccgc gcctggacgc
     7141 cggatgcatc ggctattttg tatgaaaaat actggaagct tcacggcggc gttgaagcga
     7201 tcagtaatcg tgccgacggt gttggcaaca gtttactggc actgggcgca cagtatggct
     7261 ggcaactggc agggatgatg ctcattggtg cggcgctgat gcgcagcggc tggctgaaag
     7321 ggcagttcag cttacgtcac tatcgtcgta ctggttttgt gctggtggcg attggggtga
     7381 ccattaacct tcctgccatc gccctgcaat ggcagctgga ctgggcgtat cgctggtgtg
     7441 ccttcttact tcaaatgccg cgggaactga gtgcgccgtt tcaggcgatt ggctatgcgt
     7501 cgctgtttta tggtttctgg ccgcaattaa gccgctttaa gctggtgctt gcgatcgcct
     7561 gcgtcggacg gatggcgctg accaactatc tattgcaaac gctgatttgt accacgcttt
     7621 tttaccacct cggtttgttt atgcattttg accgcctgga gctgctggcg tttgttattc
     7681 cggtatggct ggcgaatatc ctcttctctg ttatctggct gcgttacttc cgccaggggc
     7741 cggtggaatg gctctggcgt cagttaactt tgcgtgctgc cggaccggca atatctaaaa
     7801 catcaagata acgatctgga tcacattcgt taacaaaacg gctgtaaccg tttccattgc
     7861 tgtgactcga ttcacgaagt cctgtattca gtgctgacaa aatagccgcc agcaagcagt
     7921 catttactgc aatctcataa caggtagtga atatgatcac cattcgtgat gtagcgcgtc
     7981 aggctggcgt ctctgtggca acggtttccc gagtgctcaa taacagtacg ctggtcagtg
     8041 ccgacacgcg tgaagcagta atgaaagccg ttagtgagct ggattatcga ccaaatgcca
     8101 atgcccaggc gctggcaact caggttagcg acaccattgg cgtggtggtg atggacgttt
     8161 ctgatgcgtt tttcggcgcg ctggtaaaag cggtggatct ggtcgcccag cagcatcaga
     8221 aatacgtgct aatcggcaat agctatcatg aagcggaaaa agagcgtcac gccattgagg
     8281 tgttaattcg ccagcgttgt aatgcattga ttgttcactc aaaagcattg agtgacgatg
     8341 aactggcaca atttatggat aacattcccg gtatggtgtt aatcaaccgc gttgtgccgg
     8401 ggtacgccca tcgttgcgtt tgcctggata atctcagcgg tgcccgaatg gcgacgcgca
     8461 tgttgctgaa taacggtcat caacgtattg gttatctttc ttccagtcac ggcattgaag
     8521 atgacgccat gcgtaaagca ggctggatga gtgcgttgaa agagcaggat attattccgc
     8581 cggaaagctg gattggcact ggcacgccgg acatgccggg cggtgaggcg gcgatggttg
     8641 aactgctggg gcgcaatcta caacttaccg ctgtatttgc ttataacgac aatatggccg
     8701 ctggcgcact gacagcatta aaagataatg gcattgcgat tccgttacat ctctcaatca
     8761 tcggtttcga tgatattccc atcgcccgtt acaccgaccc gcaattaacg actgtgcgtt
     8821 atcccattgc ttcaatggcg aaattagcca ccgaactggc cttgcagggg gcagcaggca
     8881 atattgatcc gcgtgccagc cactgtttta tgccgacgtt agtgcgtcgc cattctgtcg
     8941 caacgcgcca gaatgcggcg gcgatcacta actcaacaaa tcaggcgatg taaccgcttt
     9001 caatctgtga gtgatttcac agtatcttaa caatgtgata gctatgattg caccgtttta
     9061 acgttgtaac ccgtatgtaa cagtgaataa tcactttcac cgaggtaata gcgtcataac
     9121 aacaattaaa gccgttttct ggagcgttac cgggcatgga agaacgaatt ttaaaaagtg
     9181 agcttcggcg ttcagtaaca cttcattaac tctactgccc cgccgagcat ttatctcaag
     9241 cactaccctg cataagaaaa accggagata ccatgaataa gaaggtgtta accctgtctg
     9301 ctgtgatggc cagcatgtta ttcggtgccg ctgcacacgc tgctgatact cgcattggtg
     9361 taacaatcta taagtacgac gataacttta tgtctgtagt gcgcaaggct attgagcaag
     9421 atgcgaaagc cgcgccagat gttcagctgc tgatgaatga ttctcagaat gaccagtcca
     9481 agcagaacga tcagatcgac gtattgctgg cgaaaggggt gaaggcactg gcaatcaacc
     9541 tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc gcgtgggcaa aacgtgccgg
     9601 tggttttctt caacaaagaa ccgtctcgta aggcgctgga tagctacgac aaagcctact
     9661 acgttggcac tgactccaaa gagtccggca ttattcaggg cgatttgatt gctaaacact
     9721 gggcggcgaa tcagggttgg gatctgaaca aagacggtca gattcagttc gtactgctga
     9781 aaggtgagcc gggccatccg gatgcagaag cacgtaccac ttacgtgatt aaagaattga
     9841 acgacaaagg catcaaaact gaacagttac agttagatac cgctatgtgg gataccgctc
     9901 aggcgaaaga taagatggac gcctggctgt ctggcccgaa cgccaacaaa atcgaagtgg
     9961 ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga agctctgaaa gcacacaaca
    10021 agtccagcat tccggtgttt ggcgtcgatg cgctgccaga agcgctggcg ctggtgaaat
    10081 ccggtgcact ggcgggcacc gtactgaacg atgctaacaa ccaggctaaa gcgacctttg
    10141 atttggcgaa aaacctggcc gatggcaaag gtgcggctga tggcaccaac tggaaaatcg
    10201 acaacaaagt ggtccgcgta ccttatgttg gcgtagataa agacaacctg gctgaattca
    10261 gcaagaaata agactgattg ggcgcagtcg tttactgcgc ccttattgta tggccaacaa
//
LOCUS       DAOTRK010000156        10167 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.157, whole genome shotgun sequence.
ACCESSION   DAOTRK010000156 DAOTRK010000000
VERSION     DAOTRK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.157"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..611
                     /gene="mepH"
                     /locus_tag="QIE41_003956"
     CDS             <1..611
                     /gene="mepH"
                     /locus_tag="QIE41_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDS3532781.1"
                     /translation="KSKTASSVKKSSITASKNAKTRSKHAVNKTASASFTEKCTKRKG
                     YKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGGSSPRTGFDCSG
                     LVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQGRGTADHVGVYV
                     GNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            739..1320
                     /gene="sodB"
                     /locus_tag="QIE41_003957"
     CDS             739..1320
                     /gene="sodB"
                     /locus_tag="QIE41_003957"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDS3532782.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1466..2635)
                     /locus_tag="QIE41_003958"
     CDS             complement(1466..2635)
                     /locus_tag="QIE41_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532783.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDISIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(2801..2890)
                     /gene="ynhF"
                     /locus_tag="QIE41_003959"
     CDS             complement(2801..2890)
                     /gene="ynhF"
                     /locus_tag="QIE41_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDS3532784.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3189..4214
                     /gene="purR"
                     /locus_tag="QIE41_003960"
     CDS             3189..4214
                     /gene="purR"
                     /locus_tag="QIE41_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDS3532785.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4211..5143)
                     /locus_tag="QIE41_003961"
     CDS             complement(4211..5143)
                     /locus_tag="QIE41_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3532786.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5256..6467
                     /locus_tag="QIE41_003962"
     CDS             5256..6467
                     /locus_tag="QIE41_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDS3532787.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            6758..7906
                     /gene="cfa"
                     /locus_tag="QIE41_003963"
     CDS             6758..7906
                     /gene="cfa"
                     /locus_tag="QIE41_003963"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDS3532788.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(7946..8587)
                     /gene="ribE"
                     /locus_tag="QIE41_003964"
     CDS             complement(7946..8587)
                     /gene="ribE"
                     /locus_tag="QIE41_003964"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDS3532789.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            8802..>10167
                     /gene="mdtK"
                     /locus_tag="QIE41_003965"
     CDS             8802..>10167
                     /gene="mdtK"
                     /locus_tag="QIE41_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDS3532790.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRA"
BASE COUNT         2444 a         2613 c         2509 g         2601 t
ORIGIN      
        1 tcaaaagcaa aaccgcttct tccgttaaaa aatcttccat taccgcttct aaaaacgcca
       61 aaactcgcag caaacacgcc gtcaataaaa cggcctcagc cagctttacc gaaaagtgta
      121 ccaaacgtaa gggctataaa tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaactc
      181 ttgccgacgc gcacaaagcg aaggtgcaaa aagccacgaa agtggcaatg aataaactga
      241 tgcagcaaat tggtaagcca tatcgttggg gtggcagctc accgcgtacc ggttttgatt
      301 gcagcggcct ggtttattac gcttataaag atttggtgaa aattcgtatt ccgcgtacgg
      361 cgaatgaaat gtatcacctg cgtgatgcag cacctatcga acgtagtgag ctgaaaaacg
      421 gcgacctggt ctttttccgt actcagggac gcggtacagc cgatcatgtc ggcgtgtatg
      481 tcggcaacgg caaatttatt cagtcaccgc gtacgggtca ggaaattcaa atcacttctc
      541 tcagtgaaga ctactggcag cgccactatg ttggcgctcg ccgggtaatg accccaaaaa
      601 cacttcgcta aaactttacc ctgttgttac ggcaacaggg taagttcatc ttttgtctca
      661 ccttttaatt tgctacccta tccatacgca caataaggct attgtacgta tgcaaattaa
      721 taataaagga gagtagcaat gtcattcgaa ttacctgcac taccatatgc taaagatgct
      781 ctggcaccgc acatttctgc ggaaaccatc gagtatcact acggcaagca tcatcagact
      841 tatgtcacta acctgaacaa cctgattaaa ggtaccgcgt ttgaaggtaa atcactggaa
      901 gagattattc gcagctctga aggtggcgta ttcaacaacg cagctcaggt ctggaaccat
      961 actttctact ggaactgcct ggcaccgaac gcgggtggcg aaccgactgg aaaagtcgct
     1021 gaagctatcg ccgcatcttt tggcagcttt gccgatttca aagcacagtt tactgatgca
     1081 gcaatcaaaa actttggttc tggctggacc tggctggtga aaaacagcga tggcaaactg
     1141 gctatcgttt caacctctaa cgcgggtact ccgctgacca ccgatgcaac tccgctgctg
     1201 accgttgatg tctgggaaca cgcttattac atcgactatc gcaatgcacg tcctggctat
     1261 ctggagcact tctgggcgct ggtgaactgg gaattcgtag caaaaaatct cgctgcataa
     1321 taactgatgg caaatgcaac attgcctggt gcgcttcgct taccatgcca actcggatga
     1381 agcgttaaca ccgcatccgg caagataaat cgcacgttgc cagtaactgt aacgcagaag
     1441 gttatccttc tgcgtttttg tttaattagc tgttggcaac gcaaactgcc tcaggttgtt
     1501 ttctggctga cataaacacc agcaataatg ccagtcccgc gacaatcgcc cccatcaccg
     1561 gcacaaagct gtatcccagc ccagcggaaa ttaccgcacc accagcagct gctcccagcg
     1621 cgtttccaag attaaaggca ccaatattga ctgatgaaga cagacctggc gcttcactgg
     1681 cgacacgcat cacgcgcatc tgtaacggcg gtacgaccgc aaaggttgca gctccccaca
     1741 ccaccatgct aatagctgcg ccgaactcat tgcgggccag gaacgggatt gccagcataa
     1801 tcaccatcag cagcaacaaa aagcctttca acgtgccgtt aactgaacga tctgccagtt
     1861 tgccgccgag atagttaccg atagagaatc cgacaccaat cagcaccagc attgccgtaa
     1921 cgaacaccgg tgttgcgtgg gtaatacttt gcagtaccgg agagatatag gtgtagaggg
     1981 taaacattgc accagctccc agtaccgtcg tcagcaatgc agacaacacc tgcggacgca
     2041 ttaataccgc cagctctttt ttcacttcag gtcgcgcccc tgcgccacct ttaggtaatg
     2101 agaagaacag acttaccatt gaaatcactc ccagccccgc cgttgccaga aatgacatcc
     2161 gccagccgat ggtttcaccc aaccaggtcg ccgccggcac gccaccgata tttgccaggg
     2221 ttaaccccat aaacatagtg gcaactgcgc tggcctgttt atgttttggc accacgcttg
     2281 cggccacaac tgaacccaaa ccaaaaaatg ctccgtgatt caggctggtc aaaatgcgtg
     2341 aaagcatcag agtcatataa tccggcgcga tggcggaaag tacattgccg agcgtgaaaa
     2401 tcgccatcag gaaaatcaac gcactgcggc gggcacgatg agaaagtaga agcgtcatca
     2461 gcggcgcgcc aaccattacg ccaactgcat aggcactgat taacattccg gcagcgggaa
     2521 tcgagatatc cacaccgcgc gcaatgacgg gcaacaagcc cattggcgag aactccgttg
     2581 tcccgatacc aaacgcgcca atcgccagcg ccagcaacgg atagttaatt ttcatgcctt
     2641 atctccacct cttcgcgtca ttacgcgata ttcattaaag tggcgaaagc atgacagcaa
     2701 tcacaaaaaa atgaaaataa caaaaagaga aaacactttt gccattttgc taacaaacag
     2761 gaaggagatg cgagggagaa cgcgctccct cgagaggaaa tcagtgcagc gcggcagtca
     2821 aacccacggc cacgatcaaa ccgaggacga taatcgttgt taccagtgaa aatttaaggt
     2881 cggtgctcat caagttttct ccttttttat taccacacaa aaagtgatat tacgcatttt
     2941 tacacactgt gatgaaaaaa tctcccgtca tttataatga taagtgtttt taccacttcc
     3001 ccttttcgtc aagatcggcc aaaattccac gcttacacta tttgcgtact ggccattgac
     3061 cccttcctga cgctccgtgt cgtttttccg gcgtaccgca acacttttgt tgtgcgtaag
     3121 gtgtgtaaag gcaaacgttt accttgcgat tttgcaggag ctgaagttag ggtctggagt
     3181 gaaatggaat ggcaacaata aaagatgtag cgaaacgagc aaacgtttcc actacaactg
     3241 tgtcacacgt gatcaacaaa acacgtttcg tcgctgaaga aacgcgcaac gccgtgtggg
     3301 cagcgatcaa agaattacac tactccccta gcgcggtggc gcgtagtctg aaggttaacc
     3361 acaccaagtc tatcggtttg ctggcgacca gcagcgaagc ggcctatttt gccgagatca
     3421 ttgaagcggt tgaaaaaaat tgcttccaga aaggttacac cctgattctg ggcaatgcgt
     3481 ggaacaatct tgagaaacag cgggcttatc tgtcgatgat ggcgcaaaaa cgcgtcgatg
     3541 gtctgctggt gatgtgttct gagtacccag agccgttgct ggcgatgctg gaagagtatc
     3601 gccatatccc gatggtggtg atggactggg gtgaagcaaa agctgacttc accgatgcgg
     3661 tcattgataa cgcgttcgaa ggcggctaca tggccgggcg ttatctgatt gaacgcggtc
     3721 accgcgaaat cggcgttatc cctggcccac tggaacgtaa caccggcgca ggccgccttg
     3781 ccggttttat gaaggcgatg gaagaagcga tgatcaaagt gccggaaagc tggattgtgc
     3841 agggtgactt tgaacctgaa tccggttatc gcgccatgca gcaaatcctg tcgcagccgc
     3901 atcgccctac tgccgtcttc tgtggtggcg atatcatggc aatgggcgca ctttgtgctg
     3961 ctgatgaaat gggtctgcgc gtcccgcagg atgtttcgct gatcgggtat gataacgtgc
     4021 gcaacgcccg ctattttacg ccggcgctga ccacgatcca tcagccaaaa gattcgctgg
     4081 gcgaaactgc gttcaatatg ctgttggatc gtatcgtcaa taaacgcgaa gaaccgcaat
     4141 ccattgaagt gcatccgcgg ttgattgaac gccgctccgt ggctgacggc ccgttccgcg
     4201 actatcgtcg ttaatcaccc gttgcgggag tctcttccgg ctcccgcagc cactccttat
     4261 tcagcgtctc actatcgccg agatactcaa gcaaccaggt taacgcaggc gacatatcat
     4321 tttgctgcca tgtcagacaa catgccgaat ccgggaaggg gttttccagt tctagtgcca
     4381 cccacttccc ctcattaagc cacggtttgg cgaaatgtgt tggcaccatc cctatgcata
     4441 atcctgcaga gatacaggtt gccgatgatt cccagtcagg cacgacaagt cttttttggt
     4501 tatccagcaa ccaggtaata cgtttaggta gcgttcgcga ggtgtcttcg cgcaccaacg
     4561 acggccagtt gcgcaacgta tcatcgctga acgggccatc catcaacgcc agcgggtggt
     4621 gactggcaac aacgcaactc cagcttagca tccccatatc gcggaaggca taacgaccgc
     4681 ctaccggaat cgcgcgtgtt gcgccaatcg ccagttccac gcgcccgtcg gaaagcgcat
     4741 cccagacacc gttgaacact tcctgaaaga caaggagttc gacatcatca aaatggcgat
     4801 aaaaatcaac gatcatctgc cgtgtacgtt ccggcctgac aatattatcc actgcgatag
     4861 ctaactgacc gcgccagccg ttcgctatct gctgacattg ctgccgggtg atctgcattt
     4921 ttttgacaac agagcgccct tctttgagaa accacgcccc ggcagcggtc agttccacat
     4981 cacggtgacg tcgttcaaac agcggcaccg ccagccactc ttccagctga cgcacggtat
     5041 agctgaccgc agaaggaacg cgatgcaact cctgtgccgc agcgctaaaa ctaccattac
     5101 gcgctaccgc atcaacaact tcgagtgaat attctgacca catagtctgc ctgcaaaatt
     5161 tttgaaacca gtcatcaaat attaccgttt cacaacacta atttcactcc ctacactttg
     5221 cggcggtgtt taattgagag atttagagaa tatacatgca acctgggaaa agatttttag
     5281 tctggctggc gggtttgagc gtactcggtt ttctggcaac cgatatgtat ctgcctgctt
     5341 tcgccgccat acaggccgac ctgcaaacgc ctgcgtctgc tgtcagtgcc agccttagtc
     5401 tgttccttgc cggttttgcc gcagcccagc ttctgtgggg gccgctctcc gaccgttatg
     5461 gtcgtaaacc ggtattatta atcggcctga caatttttgc gttaggtagt ctggggatgc
     5521 tgtgggtaga aaacgccgct acgctgctgg tattgcgttt tgtacaggct gtgggtgtct
     5581 gcgccgcggc ggttatctgg caagcgttag tgacggatta ttatccttca cagaaagtta
     5641 accgtatttt tgcgaccatc atgccgctgg tgggtctatc tccggcactg gctcctctgt
     5701 taggaagctg gctgctggtc catttttcct ggcaggcgat tttcgccacc ctgtttgcca
     5761 ttaccgtggt gctgattctg cctattttct ggctcaaacc cacgacgaag gcccgtaaca
     5821 atagtcagga tggtctgacc tttaccgacc tgctacgttc taaaacctat cgcggcaacg
     5881 tgctgatata tgcggcctgt tcagccagtt tttttgcatg gctgaccggc tcaccgttca
     5941 tccttagtga aatgggctac agcccggcag ttattggttt aagttatgtc ccgcaaacta
     6001 tcgcgtttct gattggtggt tatggctgtc gcgccgcact gcagaaatgg caaggcaagc
     6061 agttattacc gtggttgctg gtactgtttg ctgtcagcgt cattgcgacc tgggctgcgg
     6121 gcttcattag ccatgtgtcg ctagtcgaaa tcctgatccc attctgtgtg atggcgattg
     6181 ccaatggcgc gatctacccg attgttgtcg cccaggcgct gcgtcccttc ccacatgcaa
     6241 ctggtcgcgc cgcagcgttg caaaacactc tacaactggg tctgtgcttc ctcgcaagtc
     6301 tggtagtttc ctggcttatc agtatcagca cgccattgct caccaccacc agcgtgatgt
     6361 tatcaacagt agtgctggtt gctctgggtt acatgatgca acgttgtgaa gaagttgact
     6421 gccagaatca tggcaatgcc gaagtcgctc atagcgaatc acactgatct atatcgatat
     6481 acttatactt aggctgctaa caaaattttg ttgtatgatt gaaattagcg gcctatacta
     6541 atttcgagtt gttaaagcta cgataaatat tatgttttta cggggacagg atcgttcccg
     6601 actcgctatg gatggtcatt tcggcaaggg ttcctccttt ccctctgttc tacgtcggat
     6661 tatagactcg cggttttttc tgcgagattt ctcacaaagc ccaaaaagcg tctacgctgt
     6721 tttaaggttc tgatcaccga ccagtgatgg agaaactatg agttcatcgt gtatagaaga
     6781 agtcagtgta ccggatgaca actggtaccg tatcgccaac gaattactta gccgtgccgg
     6841 tatagccatt aacggttctg ccccggcgga tattcgtgtg aaaaaccccg atttttttaa
     6901 acgcgttctg caggaaggct ctttggggtt aggcgaaagt tatatggatg gctggtggga
     6961 atgtgaccga ctggatatgt tttttagcaa agtcttacgc gcaggtctcg agaaccaact
     7021 cccccatcat ttcaaagaca cgctgcgtat tgccggcgct cgtctcttca atcttcagag
     7081 taaaaaacgt gcctggatag tcggcaaaga gcattacgat ttgggtaatg acttgttcag
     7141 ccgcatgctt gatcccttca tgcaatattc ctgcgcttac tggaaagatg ccgataatct
     7201 ggaatctgcc cagcaggcga agctcaaaat gatttgtgaa aaattgcagt taaaaccagg
     7261 gatgcgcgta ctggatattg gctgcggctg gggcggactg gcgcactaca tggcatctaa
     7321 ttatgacgta agcgtggttg gcgtcaccat ttctgccgaa cagcaaaaaa tggctcagga
     7381 acgctgtgaa ggcctggatg tcaccatttt gctgcaagat tatcgtgacc tgaacgacca
     7441 gtttgatcgt attgtttctg tggggatgtt cgagcacgtc ggaccgaaaa attacgatac
     7501 ctattttgcg gtggtggatc gtaatttgaa accggaaggc atattcctgc tccatactat
     7561 cggttcgaaa aaaaccgatc tgaatgttga tccctggatt aataaatata tttttccgaa
     7621 cggttgcctg ccctctgtac gccagattgc tcagtccagc gaaccccact ttgtgatgga
     7681 agactggcat aacttcggtg ctgattacga tactacgttg atggcgtggt atgaacgatt
     7741 cctcgccgca tggccagaaa ttgcggataa ctacagtgaa cgctttaaac ggatgtttac
     7801 ctattatctg aatgcctgtg cgggtgcttt ccgcgcccgt gatattcagc tctggcaggt
     7861 ggtgttctca cgcggtgttg aaaacggcct tcgagtggct cgctaaaggc tattctatcg
     7921 ccccctctcc gggggcgatt tcagatcagg cttctgtacc tggttgattc atggcatttt
     7981 ctcgtgccgc cagcacacgt tctacggtat ctaccactgc ctgagtttgt ggatcaattt
     8041 caatgttgac gcgtgcgccg agttttttct tcccaagagt cgtgcgttcc agggtttccg
     8101 gaattaaatg gacgcaaaaa cgcgttggcg tgacttcgcc gacggtcagg ctaataccgt
     8161 cgatgccaat aaatcctttg tacagaatat atttcatcaa ctgactatcc tggactttaa
     8221 accagatctg gcgattattt tccgaggtta atattttcgc cacttccgcg gtggtcataa
     8281 tatgacctga cattaagtgt ccgccaattt catcactgaa tttcgccgca cgctcaacgt
     8341 ttacccaatc ccccactttt aaatcgccaa gattggtaat gcgtaacgtt tctttcatca
     8401 ggtcaaaact gacatggttg ccgttaattt ccgtcacggt caggcagcaa ccgttatgcg
     8461 ccacggacgc accggtttcc aggccgtcca gcatgtggtc gggtaactcc accacatgtg
     8521 tgcgaaaatt tggtttctcg tcaatcgaca ccagttttgc ggtgccctgt acaatccccg
     8581 taaacatact tacaactcct gaaatcagtt aagacattct gttcagcaca atagcaggtg
     8641 gcaaacgccc ttaccagtga aggggtaaga atggctattt tttcactgga gaattaataa
     8701 atcctcgcta caatagactg aatttcccct gcttcttctt tttgctgccc attcaggcgg
     8761 ctttttagtc tctcatataa ctacaaataa aaggtgttca cgtgcagaag tatatcagtg
     8821 aagcgcgtct gttattagca ttagcaatcc cggtgattct cgcgcaaatc gcccaaactg
     8881 cgatgggttt tgtcgatacc gtgatggcgg gcggctatag tgccaccgac atggcggcgg
     8941 tcgctatcgg tacttctatc tggcttccgg cgatcctctt tggtcacgga ctgctgctgg
     9001 cattaacgcc ggttatcgcg caattaaatg gttccggtcg acgtgagcgc attgcgcatc
     9061 aggtgcgaca aggtttctgg ctggcgggtt ttgtttccgt tctcattatg ctggtgctgt
     9121 ggaatgcagg ttacattatc cgctccatgg aaaacatcga tccggctctg gcggacaaag
     9181 ccgtgggtta tctgcgtgcg ttgctatggg gcgcgccggg ctatcttttc ttccaggttg
     9241 cccgtaacca gtgtgaaggt ctggcaaaaa ccaagccggg tatggtgatg ggctttatcg
     9301 gcctgctggt gaacattccg gtgaactata tctttattta cggtcatttc ggtatgcctg
     9361 agctcggtgg cgttggttgt ggcgtggcta ctgctgcggt gtattgggtc atgttccttg
     9421 ccatggtttc ttacattaaa cgcgcccgct cgatgcgcga tattcgtaac gaaaaaggca
     9481 ctgcgaagcc cgatcctgcg gttatgaaac gactgattca actcggtttg ccaattgcgc
     9541 tggcgctgtt ctttgaagta acgctgtttg ccgtcgtggc tctgttagtg tctccgctcg
     9601 gtattgttga tgtcgcagga caccagattg ccctgaactt tagttcactg atgtttgtgc
     9661 ttccaatgtc gctggcggca gcggtaacta tccgcgtagg ttatcgtctg ggtcagggct
     9721 caacgctgga tgcgcaaacc gctgcgcgga ccgggcttat ggtgggtgtc tgtatggcaa
     9781 ccctgacggc cattttcacg gtttcactgc gggagcaaat cgccctgttg tacaacgaca
     9841 atcccgaggt tgtaacgctg gctgcgcatt tgatgttgct ggcggcggta tatcagattt
     9901 ctgactctat ccaggtgatt ggcagtggga ttttgcgtgg ttataaagat acgcgttcca
     9961 ttttctatat cacctttacg gcttactggg tgctgggctt gccaagcggc tatattctgg
    10021 cactgaccga tctggtcgtt gaacctatgg ggccagcagg cttctggata ggctttatta
    10081 ttggcctgac gtcggcagcc attatgatga tgctgcgtat gcggttcctg caacgtctgc
    10141 cgtcagccat cattctgcaa cgagcag
//
LOCUS       DAOTRK010000157         9997 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.158, whole genome shotgun sequence.
ACCESSION   DAOTRK010000157 DAOTRK010000000
VERSION     DAOTRK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.158"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            68..643
                     /gene="ssuE"
                     /locus_tag="QIE41_003966"
     CDS             68..643
                     /gene="ssuE"
                     /locus_tag="QIE41_003966"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDS3532791.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKNFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            636..1595
                     /gene="ssuA"
                     /locus_tag="QIE41_003967"
     CDS             636..1595
                     /gene="ssuA"
                     /locus_tag="QIE41_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDS3532792.1"
                     /translation="MRNIIQLALAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVPENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1592..2737
                     /gene="ssuD"
                     /locus_tag="QIE41_003968"
     CDS             1592..2737
                     /gene="ssuD"
                     /locus_tag="QIE41_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDS3532793.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2749..3540
                     /gene="ssuC"
                     /locus_tag="QIE41_003969"
     CDS             2749..3540
                     /gene="ssuC"
                     /locus_tag="QIE41_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDS3532794.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3537..4304
                     /gene="ssuB"
                     /locus_tag="QIE41_003970"
     CDS             3537..4304
                     /gene="ssuB"
                     /locus_tag="QIE41_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDS3532795.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLQGQWRDAARRALAAVGLENRAREWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4346..6958)
                     /gene="pepN"
                     /locus_tag="QIE41_003971"
     CDS             complement(4346..6958)
                     /gene="pepN"
                     /locus_tag="QIE41_003971"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDS3532796.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     NGASDAPLRLDGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAT
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7224..8426
                     /gene="pncB"
                     /locus_tag="QIE41_003972"
     CDS             7224..8426
                     /gene="pncB"
                     /locus_tag="QIE41_003972"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDS3532797.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8596..9996
                     /gene="asnS"
                     /locus_tag="QIE41_003973"
     CDS             8596..9996
                     /gene="asnS"
                     /locus_tag="QIE41_003973"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDS3532798.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2239 a         2612 c         2719 g         2427 t
ORIGIN      
        1 tccgacagac tgaccgaatt gttatcaatg ttaacaaaac aagaacaatt ggttataagg
       61 agagagtatg cgtgtcatca ccctggcggg tagtcctcgt tttccttctc gctccagctc
      121 cttgctggaa tatgcgcggg aaaaactgaa tggcctggat gtagaggttt atcactggaa
      181 tctgcaaaac tttgccccgg aagatctgct ttatgctcgt ttcgatagtc cggcactcaa
      241 gaacttcacc gaacaactgc aacaggccga tggactgata gtcgccacgc ctgtgtataa
      301 agccgcctat tccggtgcgc tgaaaaccct gctcgacctg ctgccagaac gcgctttgca
      361 aggcaaagtg gtactgccac tggcgacggg cggcacggtg gctcatctgc tggcggtcga
      421 ttacgccctt aaaccagttt taagtgcgct gaaagcccag gagatcctgc acggcgtgtt
      481 tgccgatgac tcgcaagtaa ttgattacca ccacaaaccc cagttcacgc caaatctgca
      541 aacccgtctt gataccgcgc tggaaacttt ctggcaggca ttgcaccgtc gggatgttca
      601 ggttcctgac cttctgtctc tgcgaggtaa tgcccatgcg taacatcatt caactggcgc
      661 tggcgggatt acttagcgtc tctacgattg cggttgctgc agaatcctct cctgaagcgt
      721 tacgtatagg ctatcagaaa ggcagtattg gtatggtgct ggcaaaaagc caccagttac
      781 tggaaaaacg ctatccgcaa accaaaatct cctgggtgga gttccccgct ggcccacaga
      841 tgctggaggc gttaaacgtt ggcagtattg atctcggcag taccggagat attccgccaa
      901 tctttgccca ggctgccggg gctgatttgg tgtacgtggg cgtcgagccg ccgaagccca
      961 aagccgaagt gattctggtg ccagaaaaca gcccgatcaa aaccgtagcc gatcttaaag
     1021 gtcacaaagt tgcctttcag aaaggttcca gctcgcacaa tcttttacta cgcgcactac
     1081 gccaggccgg gcttaaattc acggatatcc agcccactta cctgacgcct gccgatgccc
     1141 gcgccgcgtt ccagcaaggt aacgtcgacg catgggctat ctgggatccc tactactccg
     1201 ctgcattatt acagggcggc gtgcgggtgc taaaagacgg taccgatctc aatcaaaccg
     1261 gatcgtttta tctggctgcc cgtccgtatg cagaaaaaaa cggcgctttt attcagggcg
     1321 tactggcaac ctttagtgag gccgatgcgt taacccgcag ccagcgcgag caaagcatcg
     1381 ctttactggc aaaaacgatg ggcttaccgg ccccggtgat tgcctcttat ttagatcatc
     1441 gcccccctac caccatcaaa ccggttaacg ccgaagttgc cgccttacag cagcaaacgg
     1501 cagatctgtt ttatgaaaat cgtctggtgc ctaaaaaagt cgatattcgc cagcgcatct
     1561 ggcagcccac tcaactggaa ggaaaacaat tatgagtctg aatatgttct ggtttttacc
     1621 gacccatggt gacgggcact atctgggaac ggaagaaggt tcacgtccgg ttgatcacgg
     1681 ttatctgcaa caaattgcgc aagcggcgga tcgtctgggt tataccggtg tgctgatccc
     1741 aacaggacga tcgtgtgaag atgcgtggct ggtggcggcg tcgatgatcc cggtgacgca
     1801 acggctgaag tttcttgtcg ccctgcgtcc cagcgtaacc tcacctaccg ttgccgcccg
     1861 ccaggccgcc acgcttgacc gtctctcgaa tggtcgtgcg ttgtttaatc tggtcacagg
     1921 cagcgatcca caagagctgg caggcgacgg cgtgttcctt gatcatagcg agcgctacga
     1981 agcctcggcg gaatttaccc aggtctggcg gcgcttattg ctgggggaaa ccgtcaattt
     2041 caacggtaaa catattcatg tgcgcggagc caaactactc ttcccgccga ttcaacagcc
     2101 gtatcctccg ctttactttg gcggatcgtc agatgtcgcc caggagctgg cggcagaaca
     2161 agttgatctc tacctcacct ggggcgaacc gccggaactt gttaaagaga aaatcgaaca
     2221 agtgcgggcg aaagctgccg cgcatggacg caaaattcgt ttcggtattc gtctgcatgt
     2281 gattgttcgt gaaaccaacg acgaagcgtg gcaggccgcc gagcggttaa tctcgcatct
     2341 tgatgatgag accatcgcca aagcacaagc cgcatttgcc cggacggatt ccgtaggaca
     2401 acagcgaatg gcggcgctac ataacggcaa gcgcgacaat ctggagatca gccccaattt
     2461 atgggcgggc gttggcttag tgcgcggcgg tgccgggacg gcgctggtgg gcgatggtcc
     2521 gacggtcgcc gcgcgaatca acgaatacgc tgcacttggc atcgacagtt ttgtgctttc
     2581 gggctacccg catctggaag aagcgtatcg ggttggcgaa ttgctgttcc cgcatctgga
     2641 tgtcgccatc ccggaaattc cccagccgca gccgctgaat ccgcaaggcg aagcggtggc
     2701 aaatgatttt atcccccgta aagtcgcgca aagctaagga acacgataat ggcaacgcca
     2761 gtgaaaaagt ggttattgcg cgttgccccc tggtttttac cggtgggcat cgtggcggtg
     2821 tggcaactgg cctcctcagt tggctggctg tcgacgcgta ttttgccctc tccggaagga
     2881 gtggtgacgg cgttctggac gctctccgcc agcggcgaat tgtggcagca tctggcgatc
     2941 agctcctggc gggcgctcat tggcttttca attggcggat cgctgggctt gattctggga
     3001 ctgattagcg ggctgtcgcg ctggggagaa cggctgctgg atacctcaat tcagatgttg
     3061 cgcaacgtgc cgcatctggc gctgatcccg ctggtgattt tgtggtttgg cattgatgaa
     3121 tccgcaaaaa tctttctggt ggcgcttggc acgctattcc ccatttatat caacacctgg
     3181 catggcatcc gtaatatcga tcgcgggctg gtggagatgg cgcgtagcta tggattatcc
     3241 ggcataccgc tgtttatcca tgtgatcctg cctggcgccc tgccctcaat tatggtcggc
     3301 gtgcgttttg cgttgggcct gatgtggctg acactgatcg ttgccgaaac tatttctgcc
     3361 aattccggca ttggttatct ggcgatgaat gcgcgggagt ttctgcaaac ggacgtggtg
     3421 gttgtcgcta ttattcttta cgccctgctc ggcaaacttg ctgacgtcag cgcgcagctg
     3481 ttagagcgcc tctggttgcg ctggaacccg gcttatcatt tgaaggaggc cacggtatga
     3541 atactgctcg tctgaaccag ggcacaccat tgttgctcaa tgcagtaagc aaacattacg
     3601 cagaaaatat cgtcctgaac caactggatt tacatattcc ggcaggtcag tttgtggcgg
     3661 tggtgggccg cagcggtggt ggcaaaagta ccttgctgcg cctgctggca ggtctggaaa
     3721 cgccaaccgc aggcgatgtg ttagcgggta ccacaccgct ggctgagatt caggaagaca
     3781 cgcgaatgat gtttcaggat gcgcgtctgc taccgtggaa atcggtgatt gataacgttg
     3841 ggctgggcct tcaaggtcag tggcgcgatg ccgcgcgtcg ggcgctggct gcggtagggc
     3901 tggagaatcg cgcgcgggaa tggcctgccg cactttctgg cgggcagaaa cagcgtgtgg
     3961 cgctggcaag agcgttgatt catcgaccgg gattgttgtt gcttgatgaa ccgctcgggg
     4021 cgctggacgc cttaacgcga ctggagatgc aggatttgat tgtgtcactt tggcaggagc
     4081 acggctttac cgtactgttg gtgacgcatg atgtgagcga agcggtagca atggctgacc
     4141 gggtgttgtt aattgaagag ggaaaaattg gtctggattt gacggtggat atcccccgtc
     4201 cgcgccggtt agggtcggtg cgattagcgg agctggaagc ggaagtgttg caacgggtga
     4261 tgcagcgtgg tgaatctgaa actcgcctga gaaaacaggg gtaaattccc gaatggcggc
     4321 gctattgccg ccattcggtt attttttaag ccagtgcttt agttatcttc tcgtacagat
     4381 cgccagagag attttccagc cctttcaact gttccagcgc cgcgcgcatt ttctcctgac
     4441 gtttggcatc ataacgtttc aggcgaatca gcggctcaat caggcgtgaa gccacctgcg
     4501 ggttacggct gttgagatcg gttagcattt ccaccaggaa ctggtaaccg ctgccatctt
     4561 cggcatggaa cgctgccggg ttgctgcccg caaacgcacc aatcaacgaa cgaatacggt
     4621 tcgggttgct catggtaaat gagcgatgct gcaacaggcc acgcaccgtc tccagcacat
     4681 tcgccgccgg gctggtggct tgcaggataa accatttatc catcaccaga ccatcctgat
     4741 gccacttgtc gtcgtactcc tgcatcagcg cgtcacggca aggcagctgt gcagcaaccg
     4801 ccgcagaaag cgctgccagc gcatcggtca tattgtttgc ttcatggtat tgcttgctca
     4861 ccagcacgtc agccagatgc gtttcaccaa aagcaaggaa gcgcaggcag gcattacgca
     4921 gagtgcgttt tgcaatatct tcatgctcaa cacggtactc gctctggtaa ttcgcgttgt
     4981 aaatagccag tagctcatcc gccagttcag tcgccagagt acgagtgagt gcttcgcgta
     5041 cttcagcaat agcaatcggg tcgatgatat cgaacagttc tgccatttca ttgacggaag
     5101 gcagcgtcag gatttctgcc gccagtgccg gatcaatctt ctcatcaagc agcaccgcgc
     5161 ggaaagcgtc agccacatgc accggcagag acagcggctg cccttgctga tgacgcgcga
     5221 cgttcagctt gatgtaggtt gccagcaggc tttgcgccgc atcccagcgg gagaaatcat
     5281 tacgcgcatg acgcatcagg aaggtcagtt gctgatcgct ccacttatat tccagtttca
     5341 ctggcgcaga gaattcgcac agcagcgcag gcaccggctg gaagtagaca ttatcaaaga
     5401 caaaggtctg ttccgcctgg gtgacgttca gcaccgaatt gaccggatga ccgcctttct
     5461 gcaacgggat cactttgcct tcgttatcat acagttcgat ggcaaacgga atatgcagcg
     5521 gctgtttttc tgcctgatcc ggcgtagctg gcgtgcgctg gctgatggtc agggtgtact
     5581 gctcggtttc tggattgtag tcgtctttga cggtcacaac cggcgtaccg gactggctgt
     5641 accaacggcg gaaatgagag agatcgacat tcgatgcatc ttccatcgcc tgcacaaagt
     5701 cgtcacaggt cgctgcacta ccatcatgac gctcgaaata aagctgcatc cctttctgga
     5761 agttttcttc gcccagcagg gtgtggatca tgcgaatcac ttccgcgccc ttctcgtaaa
     5821 cggtcagggt gtagaagttg ttcatctcaa tgaccatatc cgggcggatc gggtgcgcca
     5881 tcgggctggc gtcttccgca aactgcaatc cgcgcatggt gcgcacgttg ttgatacggt
     5941 ttaccgcgcg ggaaccaagg tcagagctga actcctgatc gcggaacacg gttaaacctt
     6001 ctttcaggct gagctggaac cagtcgcggc aggtcacacg gttaccggtc cagttgtgga
     6061 aatattcgtg accaataaca cgctcaatat cgaggtaatc tttatcggtg gcggtgtcgg
     6121 tacgggccag cacatatttg gagttaaaga tattcagacc tttattctcc attgcgccca
     6181 tattgaagaa atccaccgcg acgatcatat agatgtcgag gtcatactcc aggccgaagc
     6241 gttcttcatc ccatttcatg gagtttttca gcgaggtcat cgcccacggc gcacgatcaa
     6301 ggttgccgcg atcgacgtac agctccagcg caacttcgcg accagaacgc gtggtgaagg
     6361 tgtcgcgcag cacgtcgaag tcgcctgcca ccaacgcaaa caggtagcac ggtttcggga
     6421 acgggtcctg ccactgtacc caatgacgtc cgttttccag ttcgccttgc gcaacgcggt
     6481 taccgttgga aagcaggaag ggatatttgg ttttatcggc aataattttg gtggtaaaac
     6541 gcgccagtac gtccgggcgg tcaagataat acgtaatatg gcggaaacct tcggcttcac
     6601 actgagtgca aagcgcatcg cctgactgat aaagcccttc cagcgcggta ttcgtcgccg
     6661 ggctaatttc attaacgatc ttaagcgtaa aacgctccgg caaattactg atgaccagtg
     6721 cgccctcttc ttctttccag gcggtccacg gctcatcatt aatatgaaca gaaaccagtt
     6781 taagatcttc gccatcaaga cgaagcggag catctgatgc accattacgg acagcctggc
     6841 tgaccgcggt aacgaccgtc ttttgcgcgt ccaggtcaaa ggtcaagtca atatcagtaa
     6901 tctggtaatc cggcgcacga taatcgtgac ggtatttggc ttgtggctgt tgagtcataa
     6961 ataaccttta gcatctttta tagagtctgg tgttcagtct attcctgttg cgtaaatcgc
     7021 gctatgcaga atcttcatct tttcaggtac aaacgccttt attgctacat ttttataaca
     7081 tgcaccgcgt aatgccatcg accagaaagg tggcatatgg tgtgatcggg gttcaataaa
     7141 ttgcgaaaca aggtatactc cagcagttcc tgaagatgtt tattgtacta aacgctcctg
     7201 tacgaggacg ctactgcgca cctatgacac aattcgcttc tcctgttctg cactcgttgc
     7261 tggatacaga tgcttataag ttgcatatgc agcaagccgt gtttcatcac tattacgatg
     7321 tgcatgtcgc ggcggagttt cgttgccgag gtgacgatct gctgggtatt tatgccgatg
     7381 ctattcgtga acaggttcag gcgatgcagc acctgcgcct gcaggatgat gaatatcaat
     7441 ggctctcagc cctgcctttc tttaaggccg actatcttaa ctggctacgc gagttccgct
     7501 ttaacccgga acaagtcacc gtgtccaacg ataatggcaa gctggatatt cgtttaagcg
     7561 gcccgtggcg tgaagtcatc ctctgggaag ttcctttgct ggcggttatc agtgaaatgg
     7621 tacatcgcta tcgctcaccg caggccgacg ttgcgcaagc cctcgacacg ctggaaagca
     7681 aattagccga cttctcggca ttaaccgccg gtcttgatat gtcgcgcttc catctgatgg
     7741 attttggcac ccgccgacgt ttttctcgtg aagtacaaga aaccatcgtg aaacgtctcc
     7801 aacaggaatc ctggttcgtg ggcaccagca actacgatct ggcgcgtcgg ctttccctca
     7861 cgccaatggg aacacaagca cacgaatggt tccaggcgca tcagcaaatc agcccggatc
     7921 tagccaacag ccagcgagct gcacttgctg cctggctgga agaatatccc gaccaacttg
     7981 gcattgcatt aaccgactgc atcactatgg atgctttcct gcgtgatttc ggtgtcgagt
     8041 tcgccagtcg ctatcagggc ctgcgtcatg actccggcga cccggttgaa tggggtgaaa
     8101 aagccattgc acattatgag aagctgggaa ttgatccaca gagtaaaacg ctggttttct
     8161 ctgacaatct ggatttacgc aaagcggttg agctataccg ccacttctct tcccgcgtgc
     8221 aattaagttt tggtattggg actcgcctga cctgcgatat cccccaggta aaacccctga
     8281 atattgtgat taagttggta gagtgtaacg gtaaaccggt ggcgaaactt tctgacagcc
     8341 ctggcaaaac tatctgccac gataaagcgt ttgttcgggc gctgcgcaaa gcgttcgacc
     8401 ttccgcatat taaaaaagcc agttaatatc atcagggagc taatcggctc ccttttttta
     8461 cctttaattc cgaaatcttt cgctgcattt gcgaattctg cttgtctgat tgcagatgcc
     8521 aggtaacata ggtatccccc ccatttacgg gtggacaagt gtttattttt tccgactatt
     8581 aacagagaga atattatgag cgttgtgcct gtagccgacg tactccaggg ccgcgtagcc
     8641 gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc gccgagattc aaaagctggc
     8701 atctccttcc tcgccgttta tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat
     8761 aattctctgc ccaattacaa tgaagacgtc ctgcgtctga ccaccggttg ctcggtcatt
     8821 gtgacgggta aagtcgtggc atcgccgggc caggggcaac aatttgaaat tcaggccagc
     8881 aaggttgaag ttgctggttg ggttgaagat ccagacactt acccgatggc ggcaaaacgc
     8941 cacagcattg agtatctgcg tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt
     9001 gccgtcgcgc gcgttcgcca tacgctggcg caggcgctgc atcgcttctt taacgagcag
     9061 ggattcttct gggtttcaac gccactgatt actgcgtctg ataccgaagg ggctggcgaa
     9121 atgttccgcg tttctacgct ggatctggaa aacctgccgc gtaacgatca gggcaaagtg
     9181 gatttcgaca aagacttctt tggtaaagag tctttcctga ccgtatctgg ccagttgaac
     9241 ggcgaaacct acgcttgcgc attgtccaaa atttatacct tcggcccgac tttccgtgct
     9301 gaaaactcca acaccagccg tcacctggcg gaattctgga tgctggagcc ggaagtggcg
     9361 tttgctaacc tgaacgatat cgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa
     9421 gcggttctcg aagaacgcgc tgacgacatg aaattcttcg ctgaacgcgt agataaagat
     9481 gccgtttcac gtctggaacg cttcattgaa gccgattttg cgcaggtgga ttacaccgac
     9541 gcagtgacta ttctcgaaaa ctgcggcagg aagtttgaaa acccagttta ctggggcgtt
     9601 gatctctctt ctgagcatga gcgttatctg gcggaagaac actttaaagc accggtagtg
     9661 gttaaaaact atccgaaaga tattaaagcg ttctatatgc gccttaacga agacggtaaa
     9721 accgttgcgg ctatggacgt tctggctccg ggcatcggtg agatcattgg tggctcccag
     9781 cgtgaagaac gtctggacgt gctggacgag cgtatgctgg aaatgggcct gaataaagaa
     9841 gattactggt ggtatcgcga tctgcgtcgc tacggtactg ttccgcattc aggtttcggt
     9901 cttggttttg aacgcctgat tgcttacgta actggtgtgc aaaacgtgcg tgatgtgatt
     9961 ccgttcccac gtactccgcg taacgccagc ttctaat
//
LOCUS       DAOTRK010000158         9959 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.159, whole genome shotgun sequence.
ACCESSION   DAOTRK010000158 DAOTRK010000000
VERSION     DAOTRK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.159"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            21..1505
                     /gene="mglA"
                     /locus_tag="QIE41_003974"
     CDS             21..1505
                     /gene="mglA"
                     /locus_tag="QIE41_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDS3532799.1"
                     /translation="MLEMSGINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLL
                     KCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQRSVMDNMWLGRY
                     PTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVI
                     MDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIAT
                     EPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQPSIRDVSFDLHK
                     GEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANEAINHGFALVTEE
                     RRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQI
                     GSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIAELAKKGKGIIII
                     SSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            1521..2531
                     /gene="mglC"
                     /locus_tag="QIE41_003975"
     CDS             1521..2531
                     /gene="mglC"
                     /locus_tag="QIE41_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDS3532800.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(2602..3837)
                     /gene="preA"
                     /locus_tag="QIE41_003976"
     CDS             complement(2602..3837)
                     /gene="preA"
                     /locus_tag="QIE41_003976"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDS3532801.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQIRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHSVTL"
     gene            complement(3831..5069)
                     /gene="preT"
                     /locus_tag="QIE41_003977"
     CDS             complement(3831..5069)
                     /gene="preT"
                     /locus_tag="QIE41_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDS3532802.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTFTLDQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(5466..5705)
                     /locus_tag="QIE41_003978"
     CDS             complement(5466..5705)
                     /locus_tag="QIE41_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDS3532803.1"
                     /translation="MDVQHFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(5708..6427)
                     /gene="sanA"
                     /locus_tag="QIE41_003979"
     CDS             complement(5708..6427)
                     /gene="sanA"
                     /locus_tag="QIE41_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDS3532804.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(6577..7461)
                     /gene="cdd"
                     /locus_tag="QIE41_003980"
     CDS             complement(6577..7461)
                     /gene="cdd"
                     /locus_tag="QIE41_003980"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDS3532805.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(7591..8286)
                     /locus_tag="QIE41_003981"
     CDS             complement(7591..8286)
                     /locus_tag="QIE41_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDS3532806.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(8283..8681)
                     /locus_tag="QIE41_003982"
     CDS             complement(8283..8681)
                     /locus_tag="QIE41_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDS3532807.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            8920..9867
                     /gene="dusC"
                     /locus_tag="QIE41_003983"
     CDS             8920..9867
                     /gene="dusC"
                     /locus_tag="QIE41_003983"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDS3532808.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPIK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         2538 a         2484 c         2489 g         2448 t
ORIGIN      
        1 ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtct tttcctggtg
       61 ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat gcattaatgg
      121 gggaaaacgg cgcaggaaaa tcaacattat taaaatgcct gtttggtatt tatcaaaaag
      181 actccggcac cattttattc cagggtaaag agatcgattt ccattctgcc aaagaagcgc
      241 tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa cgttcggtga
      301 tggataacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat caggacaaaa
      361 tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat ccgcgtgcgc
      421 gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct
      481 ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa aaagaggtca
      541 atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct
      601 cacataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg cgcgacggtc
      661 agtggatcgc cactgaaccg ctggcaggac tgacgatgga caagatcatc gccatgatgg
      721 ttgggcgttc tcttaaccag cgtttccctg acaaagaaaa caagccgggc gaagtgattc
      781 ttgaagtgcg taacctgacg tcgctgcgcc agccgtcgat tcgcgatgtc tcgtttgatc
      841 tgcataaagg ggagatcctc ggtattgccg gtctggtggg ggcgaaacgt accgatattg
      901 ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg cacggcaaaa
      961 agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg gtaactgaag
     1021 agcgccgctc aacgggaatt tatgcctatc tggatatcgg ttttaactcg ttaatttcca
     1081 atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg aaaagtgata
     1141 cccagtgggt gattgattcg atgcgggtaa aaacaccggg gcatcggacg caaattggtt
     1201 cgctctccgg tggtaatcag caaaaggtga ttattggtcg ctggctgtta acgcaaccag
     1261 aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag tttgagatct
     1321 atcagttaat tgccgagctg gcgaagaaag gcaaggggat tattattatc tcctccgaaa
     1381 tgccagagtt gttagggata acagaccgta ttctggtcat gagtaatggt ctcgtttccg
     1441 gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc
     1501 tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt acttacctga
     1561 aagagggcgg tatttacgtc gttcttttag ttttgctggc aattattatt ttccaggatc
     1621 ctacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg gtgcgtatta
     1681 ttatcgcgct tggtgtggca gggttaattg tcacccaggg gaccgacctt tctgcggggc
     1741 gtcaagtagg gctggcggca gtggtggcag cgacattatt gcagtccatg gataacgcca
     1801 acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg attgtctgtg
     1861 ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcctatctc aacgtgacgc
     1921 cgttcattac cacgctcggc acgatgatca tcgtctatgg catcaactcg ctctattacg
     1981 actttgtcgg ggcgtcgccg atttctggtt tcgacagtgg cttctctacc tttgctcagg
     2041 gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg ctgattgcgg
     2101 tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg caagaacatt tttgctatcg
     2161 gcggtaaccc ggaagcggca aaagtttctg gtgtcaacgt cggcctgaac ctgctgatga
     2221 tctacgcgtt gtctggtgtg ttctatgcct tcggcgggat gttagaagcc ggacgtatcg
     2281 gctctgccac caacaacctc ggctttatgt atgaactgga tgctatcgcg gcgtgcgtgg
     2341 tgggcggcgt atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa
     2401 ttatttttac cgtcatcaac tatggcctga cgtacatcgg tgtaaacccg tactggcagt
     2461 acatcatcaa aggggcgatt attatcttcg ccgtggcgct ggattcactg aaatacgcgc
     2521 gtaagaagtg atgtgattgt gggtattgcc ggatgcgtcg catccggcaa tcggtgccaa
     2581 atgccggatg cgtcgctggt tttacaatgt tacagagtgt tctttctctc ccttcttaaa
     2641 cttcacttcc ccaagctcaa tacaacccac cgggcagacg tgaccacaaa gcagacaacc
     2701 cacgcatttc tcggtattac aatgcggcgt gcgtgttttc tcgctccatt ccatcgcctg
     2761 gtgaccgccg tcgtaacagg aaatataaca gcgtccacag ccaacacatt tatcgagatt
     2821 gatacgggga tagacaatat aactgcggtc taaatcttcc gccggaacaa tattgttatt
     2881 cgccagacct accatctcct gcaacgaatc aaatccctga tcggcgagat aatgcgacaa
     2941 cccgctcgcc atatcttcca ctatccgata cccgtactgc ataatgccgg tggtcacctg
     3001 taacgttgct gcgccgagca ataaaaactc cgccgcatcc tcccaggttt caatgccgcc
     3061 gataccgctg atcgggaaat cgcgcagctc tggatgggta cgtatttgct gaatgaagcg
     3121 cagggcgatc ggttttaccg ctttaccgga atatccggaa atactcgatt taccgttaac
     3181 gatcggcata ccgattttct gattaagatc gatattggtg atggatttaa cggtgttaat
     3241 cgccgcaatg ccatctgcgc cgccgcgctt cgccgccagc gccacttcgc acatatcacc
     3301 gatattcggc gtcatcttcg ccagcattgg cagcgtggaa ccacgtttta ctgcccgaca
     3361 atatttttct accagctccg ggctttgccc gacatcgcta cccatcgcat gagaagtcat
     3421 ttgcggacag gagaagttac actcgatcat atccgcgcca gcttcttgca ccagacgcgc
     3481 cagctcttcc cattgctgct cattttcgcc catgattgaa gcgatcaata ctttgtccgg
     3541 gtaatcttct ttcagccgac gcagggcggc cagattatct tccaacggat gttcagcaat
     3601 ctgctccata tttttgaagc cgataaaacc ggtatcttct ttcaccagat gatcaaaacg
     3661 cggcgagact tcgttggcga taaaaaagcc gatcgtttta aacaccacgc cgccccagcc
     3721 agtgtcgtag gctttggcac acatctcata gcagttgcct accggcgaag aagagaggca
     3781 gaacgggttg ggaaacttca cgccgcaaaa agtaatcgaa agatctttcg ttaacatgag
     3841 caagctccct ctaaatagtg atgaatcgcc tcggcggctt ctttcccggt tttcacggca
     3901 tagaccacgg ttttgtcccc ctcaacaata tcgccagcag caaagacttg cggatcgcgg
     3961 gtctggtaat tttgtgtatt gatggtgtta cgctgcggct ctaactccgc aaaggcatcc
     4021 agtctggcat gctgaccgac ggcgagaata attttatctg ccgccatcgt cagttcgccc
     4081 ggtaaccgta catgcttaaa cgtgacttta ttgccttcca cggctactgg cgtgaatcca
     4141 tcgatgatcg aaacgcccag ctcccgagcg ctggcaaact ctttttcgct tgccggaaac
     4201 tcatctaact cttcacgcgc tacgcaagtt accgcctgac agccgagaac tttcagcgtg
     4261 ctggctacgt ccatcgcgac atcaccgccg ccgataatta atgcgctttg tggaatgctg
     4321 atatcgcctt gcgcctgtcg tgcacgttgc aagaagtcga cggcaatctc aacgtcgcta
     4381 tgctcaaaca gtggtagccc cgaaccgctc gataacccca cggtgaccag taccgcgcgg
     4441 ttttccgctt tcagctgatc cagggtaaat gtgttaccga tttcgttatt gcacttaatg
     4501 gtcacgccca ttttttcaat acgggcgatt tctgcatcca gtactgattg cggtaaccgg
     4561 aattgcggaa tgccgttacg caaccagcca ccgggttgtg cttctttctc atagatcgtg
     4621 acgtcataac cctggtttgt cagcgtcaca ctggcctgca atccggcagg acctgcgcca
     4681 ataatggcga ctttgccgag cgttttagta ccgggctgat aaatttccat cccagtttgt
     4741 tgttcaaaat cagtaacaaa acgctgtaag cgaccgatat caatgggagt atcaacaccg
     4801 gcacgggtac aaccgctttg gcataatttc tccgtcgggc acactctggc acaaacggca
     4861 ccgagggcat tattttcgcg aattgtctcg gcagcgcctt taaaattacg aaagtagatt
     4921 gagcgaataa atttccccgg atcggtctgt gccgggcaag cctgactaca gggggcgtca
     4981 tggcataata aacagcgaga ggcttcttta atcgccagta acggtgtaaa agccggagtg
     5041 agttcatcca gataattttg ttgcggcatg tgatcttcct taatgctatg agtaattaag
     5101 gcgaacgcta aatatactcg tcatacttca agttgcatgt gctgcgtctg cgggttattc
     5161 ggcccatcca tgggcctcac cccttatggg gccgctgcag gcagcgttca aatccgctcc
     5221 atgcggattt gtcgctcacc ccagtcactt acttatgtaa gctcctgggg actctctcac
     5281 ttgtcgcctt cctgcaactc gaattattta gagtataaat attgcgtgct gatattaagg
     5341 ctatgcgcgt ggggaaattt aaactgtgaa aggattcaca aatagctgtg gttcagaatg
     5401 ttatatccgc agcttattga gtgaagtaat aattattctc aataattaac agaaaagaga
     5461 aaatattaac gatagaaaat aagataaaca atcatgccga tagataaaat tccgaagccg
     5521 atatatgcca ccatataagc gaagaagagt ccgggatttt ttgctcgcca gacatacacc
     5581 ggttccggtg catttttaac ccgtttatca atcgcgcctg cgcgccagcc tttccatgct
     5641 tcgcggaaac aaaatatcgg aatgaggaaa aaaacggcaa cgacaaaaaa atgctgtaca
     5701 tccataacta ctttccttgt ttcttttgca attcaagtaa ctgttcgggc gtgacggcgg
     5761 gatacccctg cgcatcttcc ggcacctggt gcatagccgg aatagggacc agcggcccta
     5821 aaaaacgcgg ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa cgggcggcaa
     5881 attcgcgaat acgtactgac agcatatcct tcggtgacgg tacggcataa cactgagcct
     5941 gaatccccat atgcagcgca ataaataatg cccgctcaca gtggaaacgt tgggtgataa
     6001 taatgaaatc attagtatcg aaaactttgc gtgtacgcac gatggaatcc agcgtacgaa
     6061 agcctgcgta atcgagaaca atatctgatg ggtcgacacc agcagcgatt aaatctttgc
     6121 gcatggtcat cggctcatta taactttgca atgcgttatc gccgctcagt aatagataat
     6181 ttaccttacc gctgttatag gcattaatcg ctccttgaat gcggtagcga taatactgat
     6241 taattacgcc agtacgataa tattttgctg tgccgagcac cacaccgacc tggcggtagg
     6301 ggagatcctg caattcgtcg tagatataag gcgcggtttt ccagctcatc cagcgatcga
     6361 ggccgagcac agtcaacagc agcaagccga tcaggactaa caggctgagg aacacgcgct
     6421 ttaacatgca ggagactcga tctaaggaaa atttccatca ggctacttta cccgctggtt
     6481 ttgcgcaaga aacggcaaac tgtttgccgg gattttcgac accaggagag ggctggcaag
     6541 ccggacaagg cgttcacgcc gcatccggca ccaggcttaa gcgagaagca cgcggtcgat
     6601 attgtgacag ccgagagctt tcagcgttgc ggaggtggca tcccactgaa tcaacggcgc
     6661 atcggctttt tctgccagaa ccgcgcgctg gatatccggg taatcataac ccttgagatt
     6721 caacagaatt aacgccccct gcaacggtgg cagagtcggg ttgaatgcgg cgttttcagc
     6781 gtagctgccg ctgaaaatac gaccgtcttt acattccagc gcgacaccgc ttggcgactt
     6841 actgtaaggc atgtgcgaac ggtttgccgc cgcaatcgcc gcctgagaaa gcgcatcacc
     6901 cgtcagcgca tagccgtgat cctgctcgtc catcagcagc gttttaatct ccagatcttt
     6961 cggcccaaag gcatccggta gatagtcacg cagcgcgtgt gcctcgcgtc ccggaagatg
     7021 aatacgcaga tccagaccgc tgttcagttc attcataaac tgacggcagt gaccacaagg
     7081 cgtgtagtta acggtgatcg ctgcaagcgc tttttcacca ctcaaccagg cgtggctgat
     7141 cgcgctttgt tcggcatgaa cggtttgctg cattgtcgca ccaataaatt ccatattggc
     7201 accaaaatac caggttccgc tcacaccgcg cgcaattgcg ccaacattaa aattcgacaa
     7261 tggcgtacgc gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg cgtcttcgtc
     7321 cagccccgtt gcgctcttca gcgatgagac ttgctccccg gtcaacaaag cggggaagta
     7381 cttgtctgcc agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt gggcaaaagc
     7441 ggtttgaaaa cgtggatgca tggcgttgcc tcataatcat gtaatgggat aacagtttac
     7501 gaacccgtta tggctttata tgtgatctga atctcattaa ttatgcaacg cgtgaaactt
     7561 acatcaaaaa tgcgcgacgc atcgcaaatt ttagcccatt actgccagaa taatcgggaa
     7621 aaggaacggt gcgatcagcg aagtaattat cccgcataac accagtgcca gcgaactaaa
     7681 cgcaccttcc tgataatcca gctcggcgca gcgcgccgta ccgagggcgt gcgaggctgt
     7741 ccccatcgcc agaccgcgcg cagctttggt acgaatacgc atcgcattaa gcaatgtatg
     7801 gccaaacaca gcgccgagga tgccgacgaa aatgacgcaa actgcgctga ttgccggaat
     7861 accgccaata ctgccgccaa ccgccattgc aataggcgtg gtgactgatt tcggcaggat
     7921 tgacgcggcg atttccggtg aagcgcccat caacaatgcc acagaagtcc ccgtcaccat
     7981 cgcaaccacg ctgccgataa aacagatggt gatgatcgat ttccagcgcg cgcggatctg
     8041 gtgtagctgc tcatataaag gataggccag cgcgacgacc gccggttgca gcagatcgtt
     8101 tagcacttcg ctacctttaa agtagctgtc gtaagagatg ccggtcagca ttaaaaaagg
     8161 aatgatcacc accattgcta ccagcaacgg gttaagcaag ggaaacttat accgtgccgc
     8221 cagtttgcgg gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc agatattcgc
     8281 catcattctt ctgatccttt ctgacctacg actttacgtt caccgtgcac cagttgcgaa
     8341 ctccagctca ccaccagaaa aaccaccagc gtactgactg cacaggaaac cactaccggg
     8401 ccaaactgtg cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat cggcacaaac
     8461 aatagcgcca tatagcgaat cagtacgtag caccccggat tgacccattt tgccggaaga
     8521 atttgcaagg ccagcaggac aaacaggatc agcatcccga tgatgctgcc cggaatggtt
     8581 accggtagca gtgaagcaat gaaaatgcct gcatacaggc aggcataaat caggacaaaa
     8641 gcacgtaaat attgccagat aatgttcagt gtcttactca taatcaacac cccagatgag
     8701 atggattcat catacaatta attcgtaaaa cgtgctaccg atcacattaa gaaatcagcg
     8761 cttatttttt cgctcccggg cattaagcaa tgagcagcga actcaggcat acatcaagca
     8821 tagagaggga ttgcagtttg cagcaatccc tctttgcaga cctttaatcc gctgaccata
     8881 ggcgctaaca tagcgcctca tttttttgcg ggtgatgata tgcgtgtgtt actggcaccg
     8941 atggagggtg tactcgactc tctggtgcgt gaattgctga ccgaagttaa cgactacgat
     9001 ctgtgcatca ccgagtttgt ccgcgtggtg gatcaactgc tgccaataaa agtctttcat
     9061 cgcatttgcc ctgagctaca aaatgccagc cggacacctt ccggtacgct ggtgcgcgtg
     9121 cagttgttag gtcagttccc acaatggctg gcagagaacg ccgcccgtgc ggtggagtta
     9181 ggttcctggg gcgtggatct caattgcggc tgcccgtcga aaacggttaa cggtagcggc
     9241 ggtggggcga cgttactcaa agatcccgaa ctcatctacc agggtgcaaa agcgatgcgt
     9301 gaagctgtac cggcgcattt gcccgtcagc gtgaaagtgc gtctgggatg ggacagcggt
     9361 gagaagaaat ttgaaatcgc cgatgcggtt caacaggctg gtgctacaga gctggtggtg
     9421 catgggcgga cgaaagagca gggttaccgc gcggagcata ttgactggca ggcgattggc
     9481 gagattcgcc agcggctgaa tattccggtg attgccaacg gtgaaatctg ggactggcag
     9541 agtgcgcaac aatgcatgac gatcagcgga tgcgacgcag tgatgattgg tcgcggggcg
     9601 ctcaatattc ccaacctgag ccgggtggta aaatataacg aaccgcgaat gccgtggccg
     9661 gaggtagttg ctttgctgca aaaatatacc cgtctggaaa agcagggcga taccgggtta
     9721 tatcacgttg cgcggattaa acagtggttg agttatttgc gtaaagaata cgatgaagca
     9781 acggaattat ttcagcatgt tcgggtgttg aataattccc ctgatattgc aagggctatt
     9841 caggcaattg atatcgagaa actctaaaaa accatagtga tgttttttga caatgtgtta
     9901 gctctttatc aataggccgc acggttgttt ggtggcgtca aaatcgccga ggcgttccc
//
LOCUS       DAOTRK010000159         9870 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.160, whole genome shotgun sequence.
ACCESSION   DAOTRK010000159 DAOTRK010000000
VERSION     DAOTRK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.160"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1..1003
                     /locus_tag="QIE41_003984"
                     /pseudo
     CDS             1..1003
                     /locus_tag="QIE41_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1103..1963)
                     /locus_tag="QIE41_003985"
     CDS             complement(1103..1963)
                     /locus_tag="QIE41_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDS3532809.1"
                     /translation="MNIAIVTINQENAATASWQAAQDFSGCTLAHWKIEPQPAVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLVSIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHAECQ"
     gene            complement(1960..2739)
                     /locus_tag="QIE41_003986"
     CDS             complement(1960..2739)
                     /locus_tag="QIE41_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDS3532810.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLELIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTENGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAVGKAAIIRKTVAAESPA
                     MQCLQLARAEQQRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(2717..4126)
                     /locus_tag="QIE41_003987"
     CDS             complement(2717..4126)
                     /locus_tag="QIE41_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532811.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGIKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(4148..5602)
                     /locus_tag="QIE41_003988"
     CDS             complement(4148..5602)
                     /locus_tag="QIE41_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDS3532812.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWTLLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(5672..6457)
                     /locus_tag="QIE41_003989"
     CDS             complement(5672..6457)
                     /locus_tag="QIE41_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS3532813.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVNILINNA
                     GICKLNKVLNFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            6776..8053
                     /locus_tag="QIE41_003990"
     CDS             6776..8053
                     /locus_tag="QIE41_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3532814.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPIAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            8080..9558
                     /locus_tag="QIE41_003991"
     CDS             8080..9558
                     /locus_tag="QIE41_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDS3532815.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKLLPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     NAMNKGIFKELTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         2529 a         2503 c         2438 g         2400 t
ORIGIN      
        1 gcatgacccg ttctgatgat acccatgaaa acgaggcaaa catgacgccg gaagagatga
       61 agaatgagct gcgttatctt cgcgcagaaa atgcatatct gaaagccatg caggagcatc
      121 tcctggaaaa aaagcgccag gagctggaga aaaaacgaaa gtcatccgga gcctgaggtg
      181 cggacactgc cagtctgacc tgttaaaggc tgcagggctg gccagaagca cgctgtacta
      241 ccagctgtca ctgcagaagg ctaaggacaa gtatgctgat gtgaaacagc tcatagcctc
      301 catcttccat gaacaccggg gatgttatgg gtaccggcgg atccattgtg agcttcagaa
      361 aagaggtctt aagttcagtg gcaaaacagt gcgaaaactg atgcaacagc tcggcctgaa
      421 gtctcctgtg cgcctgaaga aataccggtc ttaccgggga aatatgggac tcgctgcaga
      481 aaatatcctt cagcggcagt tcaaagcgga agctccctgt gagaaatggg tgacagatat
      541 caccgaattc agggctggcg gacaaaaact gtacctgtcc ccgatacttg atttgtttaa
      601 cggggaaatc gtggcgtggg aaactgcctg caggcctaca gaggagctgg tgaagcgcat
      661 gttaaataag gggctggaaa gccttgcaga aggagaaaaa ccgctgctcc acagtgatca
      721 agggtggcat taccggataa aaagttatca gtctgacctg gcagacaaag ggttagtgca
      781 gagcatgtcg cgcaagggca actgtcttga taatgcggta atggagaatt tctttggtca
      841 cctgaaagaa gaaatttact atcgccggga ctacagaaat gtagaagagc tggaaaatgc
      901 cgttaacgaa tacataactt actggaatca aaaaagaata aaactcagtc ttgggggact
      961 cagtccggta gaataccgga ctgagcatca aaaagccggt taactgaaac tgtccaggtt
     1021 ttggggttca gttcattgac gcgtcttatc aggcttacaa gtccccagcc cgaaaccgta
     1081 ggcaggatca tccggctgca cgttattggc attctgcgtg aatgtttgtc accagtgcct
     1141 ccagcaccac tttccagtca tcaaccacgc caacatctgc ctgtgaaaat accgctgcgc
     1201 tggcgtcatg attaatcgcc accacaaact tgctattgcg tacgcccgcc attaacgctg
     1261 ctgcgccgga agcgccaaca acaatacaca cctcaggtgc cagcagatgt ccagaaatac
     1321 caatcacttt ttcggcatca acgccaccgt tcatcactcg tgcccgactg tagcccactt
     1381 cggctcccag cttttctgcc agcatagcga tttcctggtt atctgcctct ccgccctgcc
     1441 ccaccaccag cacccgtctg gcctcagcta atgggtcgcg ggtaacattt ttcaggtttt
     1501 ctatgctaac cagccagtcg ggaagcgcac ctgggacaat gttcagttgc tgcataccag
     1561 agggcaacgt ggcgttttta tctgctcccg cctgacgtgc cagcgaaagg catagcgggc
     1621 gtttttctgt ttgcagcgtc gccgttagcg catttcccca gtgggatttg cgcacgctta
     1681 cggttggaat atctaacgaa gtcacctggc agatgcttgc accatgtaaa cgccaggcca
     1741 gccgggtcga cagttcatcg ccaaacgttc ccggcggaaa gagaacaacg tccgctggcg
     1801 ttcgctgcca ctgttccacc agcgcatcca gcacctgttc cgccacagcg ggctgaggtt
     1861 caatcttcca gtgcgccagg gtacaaccgc tgaaatcctg cgccgcctgc cagctcgctg
     1921 ttgcggcatt ctcctgattt attgtcacaa ttgcgatatt catggctgca tcctttggcg
     1981 taaatagtcc tgccatagct tttgcgcttt ttctgctacc gtttgcccat caatcagcgt
     2041 cgctccgcgt tgttgctctg ctcttgccag ttgcagacac tgcatcgctg gcgattcggc
     2101 agcaacagtt ttacggataa ttgctgcttt ccccaccgcc attcgctgac gcattcccgg
     2161 tactggcaag gccacttcac cgcactgacg cacggcaatc accgcaggca agcgaacccg
     2221 gcaacagcgc agcccgtttt cagtacgctg ttcaagggtg ataaacggcg cgtcgagagt
     2281 aaaacgctcc acctgggtga agcagggcca gcccagcatt tccgccagca aaaatggcgt
     2341 ttgcccattc tgcccttcgc tgctttggca gccagtaatt atcagctcca gcggattctg
     2401 gtactgccat tcggcaatat ggcgagcgac aaattccggc gcaaagcgca gatctgccgc
     2461 cgtctccagc aatacagctt cctcaaaccc aagagccata agatagcgca gccagtgcaa
     2521 cgcccgttca tcccccatgc tcaacgcggt taaagacatc gacgtaccgt tttttctctg
     2581 cgccaacagc agcgcggcgg cagcctgttc atcagcaccg agcaaacttc gcagaagcga
     2641 aacatccggt ccgctattac cctgagccgc cgcctgccac tctttttccg ccagcattcc
     2701 ggcatccggt tcggctttaa acgctaacag aatgttcatt cgcacctcca acatttcccg
     2761 ccgccaccag cgggagtgct ttggtttctg gtgcccatag ccaggtcacg acaaaaccaa
     2821 ccaacaacac tgccgccagg agcaataagg tgacttgcat tccccactgc gccagcaccc
     2881 acggcagcag gccagtactt accgccgcgc ccaaccgact catggcggtg gcaaaaccga
     2941 cgcccagcga gcgaatgtca gtaggaaaac tttccgcagg caaaatgccc accagattac
     3001 tgactgccga aatggtggtg ctgaagagaa caaaaagcag cagcgttaat gaactgccgg
     3061 aaggcaaaca agccatcact accagcgttg ccgccagcag caaaaaactt cccagcaaaa
     3121 atttgcgatg tgccagcagg tgcgtcagaa ctaatcccag cagcgcaccc acaattaaca
     3181 acgcattaag catcaggcta gcggtcaacg catcttccag accaatagtc tgggcgatag
     3241 ttggcagcca ggtataaatc acaaaccatg ggattacgag gcagacaaag aagacgctgt
     3301 taaacgctgt gcgccgccag tagcgcgaag agaacaaggt tttgatgtgt ttatgggtcg
     3361 ccgttgccac ttcatcgccc agtaaaacat ggggaccaaa atagcgatgc acgatagcgt
     3421 gagcttctgc aaaacgcccc tggcgcagta gccagcgtgg cgattctggc gttccccagc
     3481 gtaataacgt aatcaacaat gcgggcagag ctgccgatgc cagcagccag cgccaggcct
     3541 ccgggctttc gctaataaag tgatgtccgg caatacttgc cagcacatag cctacagtcc
     3601 acaccacgct gaacgcgccc agcaaaatac cgcggtggcg gcgcggggaa aattcagcca
     3661 gcaaggtgtg accgactgag taatcgcctc ccagaccaat gccaatcaaa atgcgcagtc
     3721 caataagatg ctctggcgtg gtggcaaaaa attgtaaaaa cgaagcaagc gtaatcagca
     3781 aaaagctgaa ggtgaagatt ttttgtcgcc caatatggtc ggagatccag cccagaacca
     3841 ggctgccaag aaacaaacca aggagtgctg agccgccaat cattcccgcc ataaacggcg
     3901 tcagttgcat ggcgggcgta agctgaataa tggcgtaacc aatgacgccg aggacataac
     3961 cgtcggtcag gtgtgcgccg aaagtgagcg cagcaatgcg gcagtgaaaa cggttaagtg
     4021 gtaaatcatc cattcgcacc ggtgaagtgt tcatctttat cccttcattg tccggtaata
     4081 agaacggctt ctcgcctgag aagccgaaaa ggcaacgccc cgtcatcgcg gaacgctgcc
     4141 tgacacatta tttttcaatc ggatagatag tacccgtatt cataatgccg ttaggatcga
     4201 actgcttttt cagaccttcc agcaacgtcc acgcgctgcc gtgttccagc ttgctccagt
     4261 gaacgcgatg tttaccaatg ccgtggtggt gcaccatcga accaccgagg cgaatggttt
     4321 cttcgcagat gattttgttg agcggattgt ggtacttgtc gatttcctct tccggcttac
     4381 agtcaacaac gttgtagtcg taaacgaagt acatgttggt gccgttctgg tagctgtgag
     4441 aggagtgacc gcccagcatg gtgatgtcgt cggcgtgcgg gaactcggta cgaatacggt
     4501 taataacgct ttcgtagatt tcgtgaatgc agctccagca gccggacact tcggtggtaa
     4561 agcccatgtt gccggttttg aggatctgca cacgttcggc agctacttta tccggtcccc
     4621 agttcaggtt gttaaaccag gtttcgatca gcttgctgtc cactcgctgg cattgcgggt
     4681 aacgtgccac gatttcagca atcccttcgc ccgtcgcctt cgcaatgcga gggttacctt
     4741 cagccataaa gatcagcacg cattttccgt cagcaaaatg ggtgaagtgt tgggtgccat
     4801 cttcagcgtc atacaaacga gcgatcgacg gacgataccc ttccaccatg atttcacgca
     4861 ggatgttgaa gccggttttc atgtcttcca ggatatagcc gtagaagagg ttgttttccg
     4921 gggtgaactt aaagattttc acagttactt cagtgatata gcacaatgca ccttcgttgc
     4981 cgatgatgat gtgacgaatg tccgggcctg ccgcgcggcg tggtacgttt ttaatgcgtg
     5041 ttacggtgcc atcagccaat actgcttcca gaccaacgac catatcttcg attgcgccgt
     5101 agagtgtgga gaactgcccg atactgcggg ttgctaccag gccgcccatc tgcgccagcg
     5161 gctttgactg cggagaatgc cccgtggtgt aacctttttc acgcaacgcg ttttccagca
     5221 cttccagcgg aactccgcat tgcgccgtgg cctgcatatt ctcaatatca atattaatga
     5281 tttgattcat ggcagagccg tcgagcacca ccgagttttc cacaacggtt tccagcccac
     5341 cttcggtagc ggaagcaccg gtacgcggca caccgttaat tttgtgcgca ttcataaaat
     5401 tcagcacacg ggatacttgt tctgtggaac cgagttttac gaccgctgcc ggaatcggca
     5461 aagtataaat gccatgaata tccggaaatt tgcgaaaacg gtcaatactg ttcttcttta
     5521 atactgtttc atcggtaatt acgcgatctg caccaacaat ttcctttagc tggtcgacaa
     5581 tcgctgcgcg agataaagac atagtaattc cttcctgata ataaaaatgg aataatcatt
     5641 aataagagaa aacagaaacc accgttatgg attagcgcac taaataacca ccatcaacca
     5701 ctaataaatg tccgttgaca taattcgatg ccggacttgc gaggaatacg gctgcgccca
     5761 ttaaatcctg agtatcgccc caacggtttg ccggaatatg atcaagaacg cgctgattgg
     5821 tttctggatt actgcgtgtc gccagcgtaa tatctgttgc ataatagcca ggggcaatac
     5881 catttacctg aatattatat tgacctagtt catcacagta agctttggtg aatccggcaa
     5941 gagcatgttt agtggcagaa tatgcaggtg accattgtcc acctaagtaa gagaataatg
     6001 aacagatatt aatgatttta ccgcttttct gcgggatcat aatttttgca gcttcatagc
     6061 ttaactcgaa tgcggcggtc aggttcacat caatcatcgg gtcccagtcg gcacgaccga
     6121 agttcagcac cttattcagc ttacaaatac cggcattgtt aatcagaata ttgacggtac
     6181 cgaaacgttc gcaacaggcg gcgattatct tctgcggcgc gccttctgcg gtgatatcca
     6241 cctgcatgaa gtccacctca acaccctgtt tttcaatcat ttcctttgtt tcgccgttat
     6301 ctttaacgaa gcttggaata aagatatttg cgcctgcttt ggccagcgcc atggcaaatg
     6361 cctggcctaa accgctattc ccgccggtaa caattgcggt tttacctttc agggagaaaa
     6421 aatccattga gaacgcattg agagattcga ttgacatagt tagctccaat ttccgtaagg
     6481 caaaaaaaaa gaaaggtaac ttcccccgcg agagggaagt tacctttctc attatctctg
     6541 gtaactggag ataattttta tcataagggc ataatcgcta atgtgatcgt aatcacagtg
     6601 tgatattcga attgtcaaat aattttaaaa agcagaagcg tgattactct cacacaacac
     6661 cactgctttc catgttattt cttattgcaa ttactagaga cctgagaaaa gtaatccacc
     6721 tgaaagggcg gcttactttt ctcttttttt tgtctattac aaatgggaat acgatatgca
     6781 acacaactca tatcgccgtt ggataaccct cgcgataatt agttttagcg gcggcgttag
     6841 tttcgacctg gcttatttac gttatattta tcaaattccc atagcgaaat ttatgggatt
     6901 cagcaatacc gagataggtt taataatgag tacatttggt attgcggcca ttattcttta
     6961 tgcccccagc ggcgttattg ccgataaatt ttcacaccgc aaaatgatta cttccgcgat
     7021 gatcattacc ggattactgg gtctgttaat ggcaacatat ccaccgctgt gggtaatgct
     7081 ctgtattcag gtcgcctttg cgataaccac tattttaatg ctgtggtcgg tgtcgattaa
     7141 agccgcctca ttgctgggcg atcatagcga gcaagggaaa attatgggct ggatggaagg
     7201 gctgcgcggc gtcggtgtaa tgtcgctggc ggtgtttacc atgtgggtct tttctcgctt
     7261 cgctccggat gacagcgcca gtctgaaaac ggtcatcatc atctatagtg tggtttacat
     7321 cttgttgggg attctgtgct ggttttttgt tagcgataac aacaatctgc gcagtgccaa
     7381 taacgaagaa aaacagtcat tccagcttag cgacatcctg gccgttttgc gcatcagcac
     7441 cacctggtat tgcagcatgg tgatttttgg cgtcttcacc atctacgcca ttctgagtta
     7501 ctccaccaac tatctgaccg aaatgtacgg catgtcgctg gtggcggcga gctacatggg
     7561 gattgtgatc aacaaaatct tccgcgcgct gtgcggccca cttggcggca ttatcaccac
     7621 ctacagcaaa gtgaaatccc ctacccgcgt gatccaaatc ctttccgtac tcggcttgct
     7681 ggcgttaact gccctgctcg tcacgaactc taacccgcaa tcggtcgcga tggggattgg
     7741 cctgatttta ctgctgggat tcacctgtta cgcctcacgc gggctgtact gggcctgccc
     7801 tggcgaagcg agaacaccgt cttacattat gggcaccacg gtaggtattt gttcggtgat
     7861 tggattcctg ccggatgtct tcgtttaccc gattatcggc tactggcaag acaccctgcc
     7921 cgctgcagaa gcctaccgca atatgtggct gatgggcatg gcggcgcttg ccatggtgat
     7981 cgtctttacc tttttgctgt tccaaaaaat tcgtactgct gatagcgccc ctgctatggc
     8041 tagcagcaag taagtccaga aaattacagg agaaaaatca tgtcgaaaaa atacatcata
     8101 gggattgatg gtggaagtca gagcacaaaa gtggtgatgt acgatctgga aggtaatgtg
     8161 gtttgcgaag gcaaaggctt attgcagccg atgcacacgc cagatgccga taccgcagaa
     8221 catcctgacg acgatttatg ggcatcatta tgctttgccg gtcacgattt gatgagtcag
     8281 tttgccggaa ataaagaaga tattgtcggt attggtctgg gatccatccg ttgctgccgt
     8341 gcgttattga aagccgatgg cacgccagct gcgccgttga ttagctggca ggatgcacgc
     8401 gttacacgcc cttacgaaca tacgaatcct gacgtggcat atgtcacctc tttttcgggt
     8461 tatctgacgc atcgcttaac cggcgagttt aaagacaata tcgccaacta ttttggtcag
     8521 tggccggtgg attataagac ctgggcatgg agtgaagatg ccgcagtcat ggagaagttt
     8581 aatatccccc gtcatatgct gtttgatgtg caaatgccgg gcactgttct tgggcatatc
     8641 actcctcaag ccgcgctggc gacacatttc ccggctgggc tgccggttgt ttgtaccacc
     8701 agtgataaac cggtagaagc actgggagcc gggttactgg atgatgaaac tgcggtaatt
     8761 tctcttggca cttacatcgc gttgatgatg aacggcaaat tactaccgaa agatccggta
     8821 gcgtactggc cgattatgtc ttctattccg caaaccttgc tgtatgaagg ttacggtatt
     8881 cgcaaaggca tgtggacggt gagctggcta cgcgacatgt taggcgagtc gttaattcag
     8941 gatgccaaag cacaggatct ttcaccggaa gatttgctca acaaaaaagc gtcttgtgtg
     9001 ccgccaggct gtaatggtct gatgacggtg ctggactggc tgaccaatcc gtgggaaccg
     9061 tacaaacgcg ggattatgat tggctttgat tccagcatgg attacgcatg gatatatcgt
     9121 tcgatactgg aaagcgtggc gctgacgctg aagaacaatt acgacaatat gtgtaatgaa
     9181 atgaatcact ttgcaaagca tgtgatcatt actggcggcg gttcgaacag cgatctgttt
     9241 atgcagattt ttgccgacgt gttcaacctt ccggcacgcc gtaacgccat taatggttgt
     9301 gcaagcctgg gggcagcgat taatacggcg gtaggtctgg ggctataccc ggattacgca
     9361 acggctgtcg acaaaatggt tcgcgtgaaa gatatcttta tgcctgttga gagcaacgcc
     9421 aaacgctaca acgcgatgaa taaaggcatt ttcaaggagt taaccaaaca tactgatgta
     9481 atcctgaaaa aatcgtatga agtgatgcat ggggaattgg ggaatgcgga ttcgatccag
     9541 agctggtcga atgcgtaagt gaggaagacc gggcgggaaa ctgcccggcc tgaacatacc
     9601 tgaatggtta tccccgctgg cgcggggaac ataagttcga caacttaaaa aatgataaca
     9661 agtccggttt atccccgctg atgcagggaa cataataaaa actttaacta gttcggttta
     9721 tccctgtttt tacaaggaat attaactaca ctcgatgtct ttagaataaa agacaatacg
     9781 aatcgcactt tatcaatttt cacttaaagt atgaactgga tatctttatt cataagaact
     9841 attcatcaac agcgtataga ggcgattatg
//
LOCUS       DAOTRK010000160         9836 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.161, whole genome shotgun sequence.
ACCESSION   DAOTRK010000160 DAOTRK010000000
VERSION     DAOTRK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.161"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            158..1252
                     /gene="ddlA"
                     /locus_tag="QIE41_003992"
     CDS             158..1252
                     /gene="ddlA"
                     /locus_tag="QIE41_003992"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDS3532816.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1276..1488)
                     /locus_tag="QIE41_003993"
     CDS             complement(1276..1488)
                     /locus_tag="QIE41_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDS3532817.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1748..2056
                     /locus_tag="QIE41_003994"
     CDS             1748..2056
                     /locus_tag="QIE41_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDS3532818.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHVPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2114..3208)
                     /gene="yaiW"
                     /locus_tag="QIE41_003995"
     CDS             complement(2114..3208)
                     /gene="yaiW"
                     /locus_tag="QIE41_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDS3532819.1"
                     /translation="MSRVNHLSSLSLLAVLVLVGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3221..4441)
                     /gene="sbmA"
                     /locus_tag="QIE41_003996"
     CDS             complement(3221..4441)
                     /gene="sbmA"
                     /locus_tag="QIE41_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDS3532820.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4793..5950
                     /gene="ampH"
                     /locus_tag="QIE41_003997"
     CDS             4793..5950
                     /gene="ampH"
                     /locus_tag="QIE41_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDS3532821.1"
                     /translation="MKRCLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNIRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5951..6574)
                     /gene="iprA"
                     /locus_tag="QIE41_003998"
     CDS             complement(5951..6574)
                     /gene="iprA"
                     /locus_tag="QIE41_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDS3532822.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6660..9608)
                     /gene="ehaB"
                     /locus_tag="QIE41_003999"
     CDS             complement(6660..9608)
                     /gene="ehaB"
                     /locus_tag="QIE41_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HDS3532823.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGKGWNADDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILNVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRTGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSINNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2531 a         2431 c         2353 g         2521 t
ORIGIN      
        1 gtgatcttgc gcacatcatt gagataacgc gctttcttca ccagtacgtt gcacgctttg
       61 tcagcagaag attgcaacat tctgtcagaa ggccgcgaat cccccgtttt gtcgtggtaa
      121 tgtatgcact ttgtgttaga cgggataggt ttttaagatg gaaaaactgc gggtaggaat
      181 cgtttttggt ggtaaatcag cggaacatga agtgtctctg caatcggcaa aaaacattgt
      241 cgatgccatt gataaaagtc gcttcgacgt tgtgctgctg ggcattgata aacaagggca
      301 atggcacgtc agcgatgcca gcaattatct gctaaatgca gacgatcctg cccatattgc
      361 gttgcgccct tcggcgacca gccttgcgca ggtgcctggt aaacatgaac atcagcttat
      421 cgacgcgcaa aacggtcagc cgttgccgac ggtggatgtc attttcccga ttgtccacgg
      481 tacgctgggc gaagatggtt ccttgcaggg aatgctgcgg gtcgccaatt taccgtttgt
      541 aggttctgat gttctggctt cagcggcctg tatggataaa gatgtcacca aacgtctgct
      601 gcgcgatgcc gggctgaaca ttgcgccatt tattaccctg acgcgcgcta atcgtcacaa
      661 catcagtttt gccgaagtgg agtctaaact ggggttaccg ctgtttgtaa aaccggctaa
      721 tcagggctct tctgttggtg tcagcaaagt aaccagtgaa gaacagtacg caattgccgt
      781 cgatctggcg ttcgagttcg accataaagt gatcgttgag caagggatca aaggtcgtga
      841 gatcgaatgc gcagttctgg gcaacgacaa tccgcaagcc agcacctgtg gcgagatcgt
      901 actcaccagc gatttttatg cctacgacac caagtacatt gacgaagatg gcgcgaaagt
      961 ggtagttccg gcagccattg cgccagaaat caacgataag atccgggcga ttgccgttca
     1021 ggcttatcag acgttgggat gcgcaggcat ggcgcgtgta gacgtgtttt taaccccaga
     1081 gaacgaagtg gtgatcaacg agatcaacac actgcctggc ttcaccaaca tcagtatgta
     1141 tccgaagctg tggcaagcca gcggtctggg ttacaccgac ctgatcaccc gtttgattga
     1201 gctggcgctg gagcgtcacg ctgcggataa cgcattgaaa accacaatgt aatatttaaa
     1261 cgcccgataa tattcttatt gggcgttttc agtttcttca tcgcgacgac gaataatcag
     1321 ccccgccagc cagaaactaa tcacccacgt caccagaccg acggcatagg tctgccaacc
     1381 ttttgcttca aatcccagta accccaccac gccattaaga atgaatataa ggccaatggc
     1441 gaacgcatag tagtgccagt cacggcggat ttttacaggc aagttcatgg cagctccgtt
     1501 aacgaaaaac aacatccttg catattctgc aacatccgtc tgtggcgtag acaacatttc
     1561 cgtaattgtt aataaattta acttaaggtt gtcattttgt gacttacgac gaagattcac
     1621 gcttcgggaa tattcccaac atcaatttac catgattctg atattgacaa tcttaatgac
     1681 ctgtcagact tgccataagc cgctggtatc acaccagaag agaagcagat gacttccgga
     1741 ggtttttatg gctgatttca ccctgtcaaa atcgctgttt agcggaaaat atcgcaatgc
     1801 ctcttcaacg cctggcaaca ttgcctatgc gttgtttgtg ctgttttgct tttgggctgg
     1861 ggcgcaattg ctgaacctgc tagtgcatgt gcccggcgtc tatgagcgtt taatgcaggt
     1921 ccaggaaaca ggtcgcccac gggtggaaat tggtttaggt gtcggcacca ttttcgggct
     1981 gatcccgttt ttagttggct gcctcatttt tgcagtagtg gcgctatggc tgcactggcg
     2041 acatcgccgc cagtaagatt tacttgccag ataggaccta aaacgcttta tctggcatga
     2101 cattcctgtt tgatcactgt ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa
     2161 accaggccgt agtcaggttg cgggtgattt tcgggctttc cagttgaatg ccaggcaaca
     2221 tttcgcgggg tagtgattta cccgttttag cttcggcaag ttgataaact ttcttgtaca
     2281 gcgccgtctc ctcaaaagag aagctgtcac ctttctctaa ctgacggcga atttcgctgt
     2341 cgctcatccc cagttttccc gccagtttgc gcgttgccag ttcggttttc ccgggttctt
     2401 tactgtcgta acgaattaaa tcaccgtcca gtgccagctt cacgccgctg gccttactga
     2461 ctgcgttctg aaacgcggca ttacggctgg cgtaccagcc agcgttaaaa tcagcaaaac
     2521 ggtatatcgg cgcgctatag ctggcgggat aattcagtaa atggtaagta ccaaaccaca
     2581 acccgccgcg acgactgaag acttcctgac ggactgtacc gtccattttc cacggatacc
     2641 ctttggtatg ctgttcggca aaggcaatgc tgacctgcat cggcccaccg gtgcgcaccg
     2701 gattaagcga accaaacaac gtctgcccca ttggcaccat gctgatcaga tcgtcaaaaa
     2761 tcgcgctcaa ttgcttttct gtacgcaccg aatctaaccg ttcgctatag cttttgccgt
     2821 ttggcgattt gatttttagc gcggtatgca ccagaaaagc aggaatgtgc atccgttcag
     2881 cacgacggtc aatctcttgc caggcgatct tgcttaagcc cggaacggcc ggatctgcct
     2941 gataatttga ctcctgctgc gccaccgcca gcaccgagca gacgttttcc agcgtcggtg
     3001 ccagcccctg actttcaaac gtggtggcga gatctttcgc ccaggcatcg cggtctttca
     3061 cgctcgcagg catcttctgc cgcacgacgc tcgccacatc tatcgctttt tcgccctttt
     3121 tcaacggttg cggagcctga ctactacacc ccactaaaac cagaaccgcc aacaatgaca
     3181 gcgaagatag atgattgacg cgcgacatta cttctccttt ttagctcaag gtatgggtta
     3241 cttcctgaat tttgtcacca tccagctcat gttcaaagct gcgcagacgt ttgtagatag
     3301 acatcaactc aaccagtgtg gtccatgagt taatcaggta ctggaaagct ccacgaacct
     3361 gaccaaaaac gttggtaatc tgcgtcatca ggccgagcgt aatcgtaccg gcgacaattg
     3421 acggaaacag caagaacaaa ccgaaaacgt tatcgacctg caaatagaga atgcgggcga
     3481 tgttgaaata catatagtga aaatagaggc ggaaatagtt tttccgtacg gcgctaaaca
     3541 gctcgcgtac cgtaggcggc gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt
     3601 tacggtaggc agcctctaca cgctggtttt taaactccag ccccggcagt ttgatcccta
     3661 ccactgccag taatccggtc cccatcagcg accagacgat agcggcaatc accagaccat
     3721 acggaatgtg cccgacaatt ggcagctccg gcacatgcgc ggagagcgtt accagcaccg
     3781 gcaggaaggc gatcaacgtc atgatggcgt tgataaaact gacgcccata ttctccagcg
     3841 ttgaagcaaa acgcatggtg tcttcctgca cacgctgtgc ggcaccttcg atatgacgca
     3901 gttgttgcca gttcgccatg taatattcgt tcatcgctgt acgccagcgg aacacgtagt
     3961 gactgacaaa gaagttgttc agcacactga tcaccacagc gatcagcgca atccccagaa
     4021 agacgcccac ttcgcggtaa aattgttcga tggtgacttt atgcggcgaa cttagcgcgg
     4081 tttgaatcag atcatagaac ggcgcatacc aggcgttgac ggcgaccccg acttccacca
     4141 aaaaccaggt gacgaagatg atcagtgcag taccgagtat tgaccagtat tgccaacgat
     4201 gcgggctgta gataaaccag aacaatgcaa agagtcctac acaaacaatg tagtaagcgt
     4261 aaaaaatcag gaaatccaac gaccagaaac gcgcggcgct aatcgggatt tgcccggaag
     4321 ctccggtgat acgcgccacc caatcacccc cacctgcttg ccagaagata acggcaatca
     4381 atgcccaaac aaaggccgag agaaaaaacg ttcccggctt tgggaaaaaa gacttaaaca
     4441 tatgcactcc tgctaacttc ttatcgtttt aacaactgct gtgtattaac cgcatgaccg
     4501 ctacaaagcg acagccaaat gaaaccgccc attgtgaccc atagcaaggc gcttagttcg
     4561 ccagcctgag cataagaatt gtttcgacgg atttcacaaa ccaaagattg tttacataag
     4621 cggattaaca cgcgcaggat cacaaatgca ggcattggtg agcaggaaaa agatcgtgtt
     4681 tacgcgccgt tacgttttta acactattat tgcactaatt cgccctttgc aatccatcaa
     4741 tgagtagtat aaatacgctc agttaccttc attcaatcta tggacaccac cgttgaaacg
     4801 ttgtctgctt ttttctgccg tgctgtgtgc ggcgtcattg acctccgtcc atgcggcgca
     4861 gccgatcact gaaccggagt tcgcctctga tattgtcgat cgttatgccg atcatatttt
     4921 ttacggcagc ggtgccacgg ggatggcgct ggttgttatc gacggtaacc agcgcgtctt
     4981 tcgcagttat ggcgaaacgc gacctggtaa taacattcgc ccgcagctgg attcggtcat
     5041 acgtattgct tccctcacca agctaatgac cagtgagatg ctggtgaaat tgctcgacca
     5101 ggggaccgtg aagttaaacg atccgctaag taaatatgct ccgccaggcg cacgcgtacc
     5161 gacctacaac gggacgccca ttacgctggt gaatctggca acccatacca gcgccctgcc
     5221 ccgtgaacag cccggtggcg cggcacatcg tccggtattt gtctggccaa cgcgcgagca
     5281 acgctggaaa tacctttcca ctgccaaact aaaagccgcg ccgggcagcc aggccgcgta
     5341 ctccaacctc gcattcgatc tgctggcaga tgcactggcg aatgcgtccg gcaagcctta
     5401 tacccagttg tttgaagagc agattacccg tccattgggg atgaaagaca ccacctacac
     5461 cccctcgccg gatcagtgcc gccgtttaat ggttgctgag cgcggtgcca gtccgtgcaa
     5521 taacacgctg gcggcaattg gcagcggcgg agtctattcc acgcctggcg atatgatgcg
     5581 ctggatgcag cagtatttgt catctgattt ttatcagcgc agtaaccagg cagatcgcat
     5641 gcagacgttg atttatcagc gagcgcagtt tacaaaagtg attggcatgg atgtccccgg
     5701 caaagcggat gcgcttggtt taggctgggt atatatggcc ccgaaagagg gtcgtccagg
     5761 gattattcag aagacaggtg gtggtggcgg tttcattacc tatatggcga tgatcccgca
     5821 gaagaacatt ggcgcatttg tggtggtcac tcgttcgcca ttgacgcgct ttaaaaatat
     5881 gagcgatggc atcaatgacc tggtgaccga actgagcggg aataaaccgt tggtcatccc
     5941 cgcgtcctga ttaatactct gaaggcaaac ggttgatagc gaccagtttg cctttattca
     6001 tttcgatata gccgccttta cgtaaagcag cgagaacttc tgcgacgaca gaacgcgata
     6061 tgcgtgtacg ttgatgaata taattcatca caccaatacg tgagcgtaat tcttcattcc
     6121 agtcaatcat tgataataac gttgcgcgga tttgttcgta ggaattatgc ccaatgagct
     6181 gtacgtcacg taattccaga attctatttt gccaggctaa ccagtaaaaa gcatctcgcc
     6241 agagttgatt ttgttcaata agggtaatgg tttgtttggc tggtagatga tatcccgtac
     6301 aatttccttc tgatattaat ttgtatggta tatcgttttt cattaaacca tcagccagtc
     6361 ccataatata aggagcctgg gtaataccga taagtacgtt ttcttctcta cgcagagaga
     6421 taacgccctc taaaataacg aaagtatctt cgctattgac atcacttgaa aatataactt
     6481 gcttttcatt attaaactcg aagcgcgtgc catatctgga caaacattta tcgagcttac
     6541 caaattcctg aagaggttta actacagata acatttgcgc gtcctttgca gtaatgcccg
     6601 tcaaatcctt gacgggcatt atttagatta aataatctat ttcttcagag tgaagaatat
     6661 taccaggtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc accaccaccg
     6721 aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg agtacccaga
     6781 ccaacacgta ccgcagaccc ttcagtaccg ttattgatgg aatcaccgtt cacatcgtta
     6841 tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag agcctggtct
     6901 tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat gctgtcgtaa
     6961 gactgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga ctggaacaga
     7021 ccagaaacgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa gtcgtaaccg
     7081 gctttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta agtaccgttg
     7141 ctcatggttg cagacaggtc gttgttgaag tgagagtagc ttaagctacc atcaacaaag
     7201 acgttgttcg cgaagtgagc agaagagtag atgtaggcag tctggctgtc ttgatccacc
     7261 tgaccagtac ggtcattcat gtcacctttc gcgaagccag cagccgcacc gacgatccac
     7321 ttagcgttgt taccgtcaat tttggtatca acaccgacca tgatgccgtt aacatcctga
     7381 tcatagttga tggtgccgtt gtcgccgttg aagttaccac cgaagtagct tacccatgcg
     7441 ccgccgttat cagccaggcc atgacgagag ttggtcagac gagtaccaac ggtgtcttgt
     7501 tccaggttcc agatattggt gttcgcagac gggatgctca gcgccatgtt agcgtagtcg
     7561 gtcagctcca tctgttgcag aacaacggtg ttaccacgct gttcagcctg ataggtgtat
     7621 gcacccaggt cagctttgtt agcagcagag aaggtcgcgt tgctgttttt gtcgttaacg
     7681 tagatcagct cgttgccgtt gtagtcagcg atagaaccag cacctgtagc gttgtcgata
     7741 cgaactttgt agttaccggt tgcagctgca acatggtcac cgttgttatc aacttcgttc
     7801 tgaccagctt cgatagaagc agtgttgtcg ttatcgccgt taccgttaat ggtcaggtga
     7861 ccatcagagt tcattgcgat taagccataa ccgtagtttg acttggtagt atcgttggta
     7921 cggtcgttgg tcaggtcggc atcaagtacg aagcggttgc tttcgatgtt gaacacacca
     7981 gcgtgaatgt taccactagt atcagtagtg ctggtcaggt tcagagtatc aacctgcaga
     8041 gcattcgccg cagtgttacc gacattcaga ataccgtcgt tagtcacttt gatgttgttg
     8101 gcatacagag cagcatcacc tacagcccaa ccagcagaaa cttcatcaga aatggttacg
     8161 gtactgttgt cgacagtcag tgaatcagtt gcaacatgac catcttcgtt gatgttcagc
     8221 gcagaaagac ctgtcagaga gatagagtca gaaatcagcg ttgaatcagc aacattaacc
     8281 gttgaaccac tgttgatgct cagagtatca atcagagaag ttttggtggt atcccactgt
     8341 gaaccggtgt tcagggttac attgaacaga ccgctctggt aaacttcgtt accgatgatg
     8401 tgatcaaatt cgctatattc agagtcgctg ttttctacac caacagttga tgaaggccac
     8461 aggctgttag ctgcgatgtc gatcagagtt gcagtggctt cagtattgat tccatatgta
     8521 taggagtcat ttacaccatc gtcatcagta tcaacaccaa tctgatgcgc agacattgcc
     8581 gcaccgaccc acttgctgcc attgttcagg gtcagatcca ggcgatcagt accatcccaa
     8641 ccgttagtat ctacatcacc gtcagcatca cgatagctat cacgaccatg cgggaagaag
     8701 ttctcatcaa agttacttga gaagaataca tcgcccatca gtgtggaatt atcgaatgtt
     8761 gcagtggttt gcatggcgtt atcagcagca ggatgtgcaa taacagctaa tgcgatatcg
     8821 tcagcattcc aacctttacc attgtaatcg ctggcattac ctgtattacc gaaccaacca
     8881 gttgtacctt catcagtcca tgaaccagag gtcacggttg aatctttaac agtgatggtg
     8941 ttattaaaca cttcgctgct gtttacacca gtgctgacag tgttgtcgtc gatatcttcc
     9001 cactcataac cctgagtcag cgtaatacct gctacatgag agttattaga gatggacaga
     9061 tcaacttcct gatccagagt gatggcagta ccaatgttaa atacatttac aacatgagtg
     9121 tcagcggcat tattgtaggt gccgttatat gtgtagtgtt cgtagttgtc atcgatcgtt
     9181 gaattatcaa cggtcagcgc cagacgatca tggtagtagt catccgcacg atcagcacaa
     9241 tcagttgtca tgcactcgga ataaatcatg ccgtgaatag tgctgttttt gattgtcagg
     9301 ctattggcgt tggtattagt agaaataccg tcatccagat agtaggtaga aatcacgccg
     9361 ttaacggtag cgttattaat gaccggatag atatcgccgt tgtaataact atcagcggca
     9421 tagttattcc agcctacata accatcatag taaacgtcat cagcataggt ggcgtcgttg
     9481 tagtgatgga aagtattgta agtttgacca gaaatatcgt tggtcgctgc attagcctga
     9541 gaggtgatag ccagagtgca agccaatgct aattgtgata ctacaagttt ctttttccag
     9601 gagtgcattt gtcatccctc ctcagggacg taataagttg atccatcaat tatcaatgca
     9661 tagaaaacat attatgtttc gcgttgacga ttatgcggcg ttatagagga atggttcaat
     9721 tattctttcc cctaagtccg attgcggaca tatataaagc acgtgaacac acacacagaa
     9781 agaaataaca aaaacacaat atttcaatac gttaatgaaa gcgttaattt taaaac
//
LOCUS       DAOTRK010000161         9821 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.162, whole genome shotgun sequence.
ACCESSION   DAOTRK010000161 DAOTRK010000000
VERSION     DAOTRK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.162"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..415
                     /locus_tag="QIE41_004000"
     CDS             <1..415
                     /locus_tag="QIE41_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDS3532824.1"
                     /translation="PDKETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMS
                     LVNITPDTDGTTLTLRIQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPA
                     SVLAPSDVDIPLQLKGISVDQLGFVRIHDIQPVMQ"
     gene            713..1597
                     /gene="tsx"
                     /locus_tag="QIE41_004001"
     CDS             713..1597
                     /gene="tsx"
                     /locus_tag="QIE41_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDS3532825.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(1774..2121)
                     /locus_tag="QIE41_004002"
     CDS             complement(1774..2121)
                     /locus_tag="QIE41_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDS3532826.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(2250..3221)
                     /gene="secF"
                     /locus_tag="QIE41_004003"
     CDS             complement(2250..3221)
                     /gene="secF"
                     /locus_tag="QIE41_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDS3532827.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(3232..5079)
                     /gene="secD"
                     /locus_tag="QIE41_004004"
     CDS             complement(3232..5079)
                     /gene="secD"
                     /locus_tag="QIE41_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDS3532828.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(5107..5439)
                     /gene="yajC"
                     /locus_tag="QIE41_004005"
     CDS             complement(5107..5439)
                     /gene="yajC"
                     /locus_tag="QIE41_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDS3532829.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(5462..6589)
                     /gene="tgt"
                     /locus_tag="QIE41_004006"
     CDS             complement(5462..6589)
                     /gene="tgt"
                     /locus_tag="QIE41_004006"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDS3532830.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(6645..7715)
                     /gene="queA"
                     /locus_tag="QIE41_004007"
     CDS             complement(6645..7715)
                     /gene="queA"
                     /locus_tag="QIE41_004007"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDS3532831.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRDKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMSAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            7934..8515
                     /gene="acpH"
                     /locus_tag="QIE41_004008"
     CDS             7934..8515
                     /gene="acpH"
                     /locus_tag="QIE41_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDS3532832.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(8536..9657)
                     /locus_tag="QIE41_004009"
     CDS             complement(8536..9657)
                     /locus_tag="QIE41_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532833.1"
                     /translation="MTDKNGQSIEVSYIFTDTIDNNYDGKLCLKICNPCNQNDSSTHE
                     YKIDHQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIADAGFIAGKVNADNFN
                     VKEFKDDIASMLCEQPPMRTHQISSSGYLSADKVPDLYKTNLEDAQRDLKTTPVDIDG
                     VSLKPKTREKIAEKIAKKTEEENAKKIAKQNAKTIEEKNSEKNKKETNPEDNFVLKFS
                     PEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLYNVARNGSPRYAGLLRQIA
                     SKCAILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDFPHINDNGELSR
                     TVLMFEGVAESNFKDIANHSSECFSYSCYAAPLDANAKK"
BASE COUNT         2456 a         2521 c         2307 g         2537 t
ORIGIN      
        1 accggataaa gaaacagtca aaatcactca gcaaaacagg ctgaatgcaa aatccagcag
       61 tggggtttac cttctgcctg gagcgaaaac accagcaaga ctggaaagcc agatcggtac
      121 tttacgtatg tcgctggtga atattacgcc tgatacagat ggcaccaccc tgacgctacg
      181 tattcagggc gagtcgaatg atcccttgcc cgcattcagc ggaactgttg aatatgggca
      241 aattcaggga acaatagaca actttcagga aatcaatgtg cagaatcaat tgattaacgc
      301 ccctgccagc gtcctcgccc ccagcgatgt tgatattccg ttacagttaa agggtatttc
      361 tgtggatcag ttaggctttg tacgtatcca cgacattcaa cctgttatgc agtaaacgtt
      421 tcgcggggcg ttttgtgcgt cccgcaacat ctttccccgt cattttgtta ctctgcttac
      481 atcacccaga ttgatagtaa aagtttgcaa caagggtgaa agtcagtaca atccccgccc
      541 gaatgtgtgt aaacgtgaac gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca
      601 tatttttgtg agcaatgatt tttataatag gctcctctgt atacgaaata tttagaaacg
      661 caatttgcgc ctttttcact cccgcaaggg attttcaaac agtggcatac atatgaaaaa
      721 aacattactg gcagccggtg cggtactggc gctctcttcg tcttttactg tcaacgcagc
      781 tgaaaacgac aaaccgcagt atctttccga ctggtggcac cagagcgtta acgttgtcgg
      841 aagctatcac acccgtttcg gaccgcagat ccgcaacgat acctaccttg agtacgaagc
      901 attcgctaaa aaagactggt tcgacttcta tggttatgcg gatgcgccgg tattcttcgg
      961 cggtaactcc gatgcaaaag gtatctggaa ccacggttct ccgctgttta tggaaatcga
     1021 accacgtttc tccatcgaca agctgaccaa tactgacctt agctttggtc cgttcaaaga
     1081 gtggtacttc gcgaacaact acatttacga catgggtcgt aataaagatg gtcgccagag
     1141 cacctggtac atgggtctgg gtaccgatat cgatactggc ctgccgatga gcctgtccat
     1201 gaacgtctat gcgaaatacc agtggcagaa ctatggcgca gcgaatgaaa acgagtggga
     1261 cggttaccgt ttcaaaatta aatactttgt gccgattacc gatctgtggg gcggtcagct
     1321 gagctacatc ggctttacca acttcgactg gggttccgat ttaggggatg acagcggtaa
     1381 tgcaatcaac ggtattaaga cccgtaccaa taactccatc gcttccagcc atattctggc
     1441 tctgaactac gatcactggc actactctgt cgtagctcgt tactggcacg acggtggtca
     1501 gtggaacgac gatgcagaac tgaacttcgg caacggcaac ttcaacgttc gctctaccgg
     1561 ctggggtggt tacctggtag taggttacaa cttctgatga atgaaatgcc ggatcttgta
     1621 tccggcattt cagtttgtta acaacgtgcg ctttgttcat gccggatgcg gcgtaaacgc
     1681 cttatccggc ctacaaaagc acgtaaattc aatacattgc tgagattacg ttggcctgat
     1741 aagcgtagcg catcaggcaa ttttgcgttt ttatcacttc ttcttgttca tcatcgcttt
     1801 caggtcagcg aatgggttgt acttcgcttc accgacatct ttctgcgcat cttcccctgc
     1861 gataacggtc gtaccatact gatctgcttc ggtatatttt gaatgctcgt gatcgtggca
     1921 ataaagacac aataactccc agttactgcc gtcttccggg ttattggtat ggtcgtgatc
     1981 aatgtggtga acggtaagtt cacgcaggtt ggaataaaca aactcgcgag aacagcgacc
     2041 gcagacccac ggatagattt ttaatgcttt ttcgcgatag ccgctttcta accgcgcata
     2101 gttttttggg attatagcca tgaaaagtta cctggatgaa atgtagggtt acgcgcagtt
     2161 tcccaagatg cggggatttt cgcaatctcc atacatcaaa aaaaatcccg atcttctgac
     2221 cgggattttc aacatcaacg ggaacttgat tacggcagaa ttgacggctg atccgcccct
     2281 tctttttcca ctttctgctg cagcatgtgt tcgcgcttca tacccagttt cagagccagc
     2341 gccgatgcta cgtagatgga agatgcagta ccgatggaaa caccaatcag catggtcagc
     2401 gagaagcctt ccagtaccgg accaccgaag aggtacagca tcaggataac catcaaggta
     2461 gtaccggatg tgatcaaggt acggtgcagc gtctgggtca aggacacgtt aaagatttcg
     2521 taaggcgtac cgcgacggat cttgcggaag ttttcacgaa tacggtccga taccacgata
     2581 ctgtcgttaa gcgagtaacc gataactgac atcaacgatg ccacaatggt caggtcaatc
     2641 tcgatatgga ataacgacaa aatacccagc gtaatgataa cgtcgtgcgc cagcgcaata
     2701 acaacccctg ccgccagtcg ccactcaaag cggaaaccta cgtacacgag gatagacagc
     2761 agcgccgcca tcaacgccat cgcaccggtt tgcgcaaggt ctgcccccac gctcggaccg
     2821 acgaactcaa tacgcttcac tgccgcattc tgattggtgg attcgttaat caccttcagg
     2881 acctggctgc ccagcacctg accgccggtt tcgccttcag caggcggcat acggaccatg
     2941 atgtcgtggc tgctaccaaa gttttgcagc atcggctctt caaaaccggc tttttgcaat
     3001 gcatcacgca ttacgtcaat ttcagccggt ttttcgagcg taatttcgat aaccgtacca
     3061 ccggtgaaat ccagccccca gttaaagccg cgcacgccca taataacgat agcggcgatt
     3121 aacagcagac cagagatgcc aaaagcccag tagtcccagc gcataaagtc atagacttta
     3181 cggccgtggt ttagttgttc aacagtatat tcctgtgcca catcgcactc ctcagattga
     3241 cagcttcttg acgcgcttgc cgccatatag caggtttacg atggcacgcg taccgacaat
     3301 cgcggtaaac atcgacgtcg ccacaccgat accggtagta atcgcgaacc ctttaattgc
     3361 cccggtaccc actgcgtaca ggatgatgac tttaatcagc gtggtgatgt tcgcatcgaa
     3421 gatagaactg aatgcgccac gataaccttc atcaattgcc tgttgaacag tacgtccgtt
     3481 gctcaactct tctttaatac gttcgttgat aagtacgttc gcatcgaccg ctaccgcaag
     3541 agttaagacg atacccgcga tacctggcat actcagcgtt gcgcctggca acagcgacat
     3601 aatgccgaca atcaagatca agttggcaat cagagcactg gtcgcaatca gaccaaactt
     3661 cttatagaag atgatcatga acagaataga aaccagcaga ccggcaaggc aagcctccag
     3721 cccctgctca atgttctgca tacccagggt cgggccaatt gtacgttctt caacaatctg
     3781 aatcggcgcg atcaacgcac cggcacgcag cagcagtgac agctgacggg cttcgttcgg
     3841 gttgttgatg ccggtgatac ggaagctgtt acccagacga gactggatgt tggcgatgtt
     3901 aatcacctct tcctgtttca ccagaaccgc acgaccgttt gcatctttct taccgctgtc
     3961 tttgtactcc acaaacaggg tcgccatcgg tttgccgatg ttgtccttgg tgaagttaga
     4021 cataatgttg ccacctgcgc tgtcgagcga gatgttaacc tgcggctggt tgtattcgtc
     4081 ctggctggaa gtggagtcgg tgatatggtc accggtcaga attacgcgtt tgtacagcac
     4141 aactggctga ccttcgcggg tctgtttcac ttcagagtcg ccaggtacgc gaccggatgc
     4201 cgctgcggcc tgatcaacgt tagtgtttac cagacggaat tccagcgttg ccgtcgcacc
     4261 cagaatctct ttcgcacgcg cggtgtcctg aatacctggc agttcaacaa cgatacggtc
     4321 agcaccctga cgctgaacca ccggctccgc cacaccaagt tggtttacac ggttacgcag
     4381 gatattaatg ttctgctgca ccgcatattc acgcgcttca ctaaggcgag catcgctcat
     4441 taccgcacgc agctggttgc tgccctggct actaatcacc aggtccggat gacgcttgct
     4501 cagatacgca atggcttcat cacgagcttt agcatcgcgg aaagtgatgc tcaggccgta
     4561 gttgttttct ttacgaacgg tggtatacgg gatgcccttt tcgcgcaggt cgctgcgcag
     4621 gctatcgata ttttgttcct ggagtttgcc aagcgcggtg tccatatcca cttccatcag
     4681 gaagtgaacg ccgccacgca ggtcaaggcc gagcttcatc ggctcagcgt gaatagctgc
     4741 cagccagcgc ggcgttgccg gggcaaggtt aagcgccacg acgtatttgt cacccataac
     4801 gcccattaat gcttcacgag cgcgcagctg ggtgtcagtg gagtcgaagc gcgcaagaat
     4861 agcgccctct tccagtgcca cagacttagc agttattttt tcttcttgta acgttttctg
     4921 gacctggatc agcgtttgct cactggcggc gactccgcgc gcaccagtga tctgaacagc
     4981 cggatcctca ccaaacaggt tgggaagcgc atacagcaga ccgatgacaa tcaccacgat
     5041 cagcatgacg tacttccaca aaggataacg gtttaacacg gcaattccct tagggaaaaa
     5101 ttttaattac agcgccttca tggtgccttt cggcaggacg gcagctacga agtcacgttt
     5161 aataactact tcagtggtgt cattcagcgc gatagcaatg tagccgtttt ccgctacttt
     5221 ggttacgcga ccaaccaggc caccgttcgt cagaacttca tcacctttgg caatggagtc
     5281 catcagcttt ttgtgttctt tggtgcgctt ctgctgtgga cgcaggatca tgaaatagaa
     5341 aatcagaccg aataccacca gcatcaaaat caaagacatc gggctacctt gcgccggtgc
     5401 acccgttgcc gctaccgcat cagaaataaa aaagctcatt aaatttccct cattattaat
     5461 attaatcaac gttcaaaggt ggtacttctc gcccctgacg ctggtaaaaa tcagttacga
     5521 agctctctaa tttaccctct tcaatagcct tgcgtaaacc cgccatcaaa cgctggtagt
     5581 aacgaaggtt atgaatggtg ttgagtcgcg cgcctaatat ttcgttgcaa cggtcgagat
     5641 gatgcaagta agcgcgtgaa taattgcgac aggtgtagca atcacactca ggatcgagtg
     5701 ggccagtatc gctcttatac ttcgcattgc ggattttcac cacgccatcg gtcacgaaca
     5761 aatgaccatt tcgggcgttg cgggttggca ttacgcagtc aaacatatcg ataccgcgac
     5821 gcacaccttc aaccaggtct tctggtttac caacgcccat caggtaacgc ggtttgtctg
     5881 ccggaatttg cgggcataca tgctccagaa tgcggtgcat atctgctttc ggctcaccca
     5941 cagccagacc gccgacagcg tagccatcaa aaccgatatc taccagacct ttaacagaaa
     6001 tatcacgtaa atcttcgtaa acgctgccct gaatgatacc aaacagcgca tttttgtttc
     6061 cgagactgtc aaaacgctca cggctacgct tcgcccaacg cagagacatc tccatggagc
     6121 gttttgcgta atcccagtca gcaggatacg gcgtacactc atcaaagatc atgacgatat
     6181 ccgaaccaag atcgtactga atctccatcg acttttccgg gtcgaggaaa atcgggtcgc
     6241 cgttgatcgg gttacggaag tgcacgccct gttcggtgat tttacgaata tcaccaaggc
     6301 tgaagacctg gaagccgccg gagtcggtaa ggattggtcc tttccactgc ataaaatcgt
     6361 gcagatcgcc gtgcagtttc atgatttcct ggcccgggcg cagccacagg tggaaggtgt
     6421 tgccgaggat aatttgcgcg ccagtggctt caacttcttc cggcgtcatc ccttttacgg
     6481 tgccgtaggt gccaacaggc ataaaacaag gcgtttccac tacgccacga tcaaagacca
     6541 ggcggccacg gcgtgcgcga ccgtcggtgg tgtccagctc aaatttcatt ttttctccac
     6601 tacgtcagaa aaacagtcca acgttttaaa ccagcgccgc ggaattactc cccgacgcgc
     6661 tcattaattg cctgcggatt gtacgtgata aacatcgcat caccgtaact aaaaaagcga
     6721 tatttctctt ctaccgctgc tttataggcg ctcatggtgt gttgataacc ggcaaaggcc
     6781 gaaaccaaca taatcagcgt cgactctggc aagtggaagt tcgtcaccag cgcatcgacc
     6841 actttgtact gaaagcccgg atagataaag atttgggtgt cgtcaaagaa cggttcgatg
     6901 agatcgtttt tcgccgcctg agccgcgctt tccagtgaac gtaccgaggt ggtgccaacc
     6961 gcaatcaccc ggttaccgcg cgctttcgcc gccagtaccg cgtctaccac atcctgcggt
     7021 acttcagcgt attccgagtg catgatgtga tcttcaatgg tgtcgacgcg caccggctgg
     7081 aaggtgcccg caccaacgtg cagggtgacg aacgccatct ccacgccttt gtcgcgtaat
     7141 ttttccagca aaggttcgtc aaaatgcaga ccagcggtcg gggctgcaac cgcgcccggt
     7201 ttttcgctat aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg acggtcgata
     7261 tacggcggca gcggcatatg gccgatgctg ttgaggatat ccagtaccga acgatcatca
     7321 ttaaattcaa cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt gatactttcg
     7381 tcatcgccca gtaacagttc tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca
     7441 agaatgcgtt tgtcgtcgag catccgctca accagcactt caatcttgcc gccgctggct
     7501 ttacgcccaa acaggcgcgc cgggatcacg cgggtattat taaaaaccag aagatcgccg
     7561 gggttgagct tatcgagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg
     7621 tccagcgaca gtaaacgaca gctactgcgt tcaggcatgg gatagtgggc aatcagggat
     7681 tcgggcaatt caaaggagaa atcggtaacg cgcatgacac tgactcagac taaaataaga
     7741 ggcgggtagt ctagtgccgg ggcgctctcc ctgcaacctt taccctcctt gatggatgac
     7801 ataatctata cacaaaatca ttcaagatgc atcgagatgg caactgaatg agtccccagg
     7861 agcttacatt aagtaagtga ctggggtgag tgaaggcagc caacgaagag gcagtttgaa
     7921 ggatgaagtg tatatgaatt ttttagctca cctgcatcta gctcacctgg cggaaagctc
     7981 gctttctggc aatttactgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc
     8041 cgacgtcgtg gctggcattc atatgcatcg gcgcattgat gtactaaccg acaatctgcc
     8101 ggaagtccgc gaagcacggg aatggtttcg tagtgaaacc cgccgcgttg cgcctattac
     8161 cctggatgtc atgtgggatc actttctttc ccgccactgg tcgcagctgt cgccggactt
     8221 tccgctacag gaatttgtct gttatgcccg cgaacaagtg atgacgattt tgccggactc
     8281 accgccgcgc tttatcaatc tgaacaacta cttatggtca gagcagtggc tggtgcgcta
     8341 tcgcgatatg gatttcatcc agaacgtgtt aaacggcatg gcaagccgtc gcccacgtct
     8401 ggatgccctg cgtgactcct ggtacgattt aaacgcccat tatgccgccc tcgaaacccg
     8461 cttctggcag ttttatccgc ggatgatggc gcaggcgtca cgcaaggcgc tctgaaactt
     8521 caagctccgg cgtcactatt ttttagcatt cgcatccagc ggggcggcat aacaactgta
     8581 agaaaaacat tcgctggaat gattggcgat atctttaaag ttgctttcag caaccccctc
     8641 gaacatgagt accgttctgg acaactctcc gttgtcattt atatgtggaa aatcaaattg
     8701 tgtactgaat cgccatacat ctgtcttttc tggctcgctg ttcacagaga tacgtcgatg
     8761 gaagactcct cctgaaaacg tgcgccagaa caattgttga tcaaaatctt gtaatatcgc
     8821 acacttggat gctatttgac gtagtaaacc tgcataccgt ggactgccat tacgagcaac
     8881 attatacaaa ccctgactaa aagcatcaaa caaacaatag atatgatcac tagtgaaaaa
     8941 aggtcttaat tttttttgtt cttcatcgct gtaatccagg agcatctcat tgatttcttc
     9001 cggtgagaat tttaagacaa aattatcctc aggatttgtt tctttcttgt ttttttcact
     9061 attcttctcc tcaatggtct tcgcattttg tttcgcaatt tttttcgcat tttcttcctc
     9121 agtttttttc gcaatttttt ccgcaatttt ttcccgagtt tttggtttta gactaacacc
     9181 atcgatgtca accggtgttg ttttcagatc tctctgcgcg tcttccaaat ttgtcttata
     9241 gagatcgggt actttatcgg cagataagta tccggatgaa gatatctggt gtgttcgcat
     9301 ggggggctgt tcacaaagca tcgaggctat atcgtcttta aactctttta cattaaagtt
     9361 atcggcatta accttccctg ctataaaccc cgcatcggca attttcttct ctaattgttt
     9421 tgtacgttta gaataataag cgttgttatc ataaaacttt tcaagaaggg cccactcctc
     9481 aagttgcctt atgtgatttt tcaactgttg atgatctatt ttatattcat gtgtactaga
     9541 atcattttgg ttgcaggggt tacagatttt taagcataat tttccatcat aattattatc
     9601 tatggtatca gtgaaaatat aggacacttc aatactctgc ccgttcttat cagtcatctc
     9661 atatgaccat tgccccgttc gtttcgcatt cttaatgagt tcgatggact ctacaggaca
     9721 accatgaaga atgggcagaa ttttatcagg atgcttaatg agtaaaatta ttatatttac
     9781 aatatttgaa aagaaatctt tgctattttt tatttcatcg a
//
LOCUS       DAOTRK010000162         9783 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.163, whole genome shotgun sequence.
ACCESSION   DAOTRK010000162 DAOTRK010000000
VERSION     DAOTRK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.163"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1255)
                     /locus_tag="QIE41_004010"
     CDS             complement(<1..1255)
                     /locus_tag="QIE41_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDS3532834.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGVTQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGGIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGNVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            1564..1640
                     /locus_tag="QIE41_004011"
     tRNA            1564..1640
                     /locus_tag="QIE41_004011"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1598..1600,aa:Val,seq:gac)
     gene            1645..1721
                     /locus_tag="QIE41_004012"
     tRNA            1645..1721
                     /locus_tag="QIE41_004012"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1679..1681,aa:Val,seq:gac)
     gene            1829..2134
                     /locus_tag="QIE41_004013"
     CDS             1829..2134
                     /locus_tag="QIE41_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDS3532835.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLEPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            2260..3864
                     /locus_tag="QIE41_004014"
     CDS             2260..3864
                     /locus_tag="QIE41_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDS3532836.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPGWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDKNPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(3876..4688)
                     /locus_tag="QIE41_004015"
     CDS             complement(3876..4688)
                     /locus_tag="QIE41_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532837.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(4692..5477)
                     /locus_tag="QIE41_004016"
     CDS             complement(4692..5477)
                     /locus_tag="QIE41_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDS3532838.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWFVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYIGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(5474..6142)
                     /locus_tag="QIE41_004017"
     CDS             complement(5474..6142)
                     /locus_tag="QIE41_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS3532839.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHYFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(6206..6844)
                     /locus_tag="QIE41_004018"
     CDS             complement(6206..6844)
                     /locus_tag="QIE41_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDS3532840.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDHADIACLTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIES
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(6857..8959)
                     /locus_tag="QIE41_004019"
     CDS             complement(6857..8959)
                     /locus_tag="QIE41_004019"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDS3532841.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLNIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPGTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(8980..9606)
                     /locus_tag="QIE41_004020"
     CDS             complement(8980..9606)
                     /locus_tag="QIE41_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDS3532842.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         2513 a         2585 c         2170 g         2515 t
ORIGIN      
        1 acgcaggctg gagcgttgcg ccaatcccgt cttcgtctgg ctgtagtttc agtgcgttac
       61 acagagtttg ccgactggca tctgttatct caacgtgtat atcaccagta acttcccctt
      121 tcacattgat gaagtcaggt gaaatttctt tcgcactggt ttgcaggcaa atcgctccct
      181 ctccgcgcag gcgtttcact ttcagttgca catgcgttgc cgcaccagtg attagccaca
      241 gttttccgcc atttagattg atgttaccaa cactgaaagt gacactgtca ccttttaccg
      301 tactgccgtc gagaatcaat acaccgccct gctgtacagt aatcgttgat tgttgcagat
      361 ctgcgccata gccaataatc attgaagcgc cgtccagtaa tgcaacatta ccggtcagtt
      421 caaccggtgc gcgatcttcc tctacgacaa aattttcgtc cgcgctggtg ggtgacaaca
      481 ggatgactgt cccaccttgt attaccgcat tttccagcgt accggcatgc acttcagccc
      541 gcccccccac attaacggac aggttttgcg tcttaccgga actgtagagc tgaagaccat
      601 ccgtacccag acgatagatg cctccattct caacaatagt gtcagagtct tcaccaccga
      661 gattattcat ggtgccacca gcaagcaacg tatcattggc tttccccgcc agcataaaaa
      721 cgccgccctg attaatctca caattgctgg cttccccacc tggatacacc aacaattcac
      781 caccttcatc taccacgacc gctgaggttg tcccattgac caacaggcca ccctcttgat
      841 cgagaataat tttttccgcg cgatggcctt ccagtacacg caggttaccg ccattttcca
      901 gcaacaaacc gcaggcataa cctttatcaa cgctgaattc accttcggga tggcggccat
      961 taaccgtagc gagcgtagag agactaattg cgcctccctc ggactgaaca atatctgtgg
     1021 caatggcacc gtcgctgacc gatagcgtgc cattgttgcc gtactgtgtg catttggcga
     1081 tcccaccggt tgccactttc aaccagccag cccccgtgac cacggtattt tcggctcttc
     1141 cgccagcttc aacaattaac tcaccttcag taagttgcgt cacgccaatg acctcgccgg
     1201 tcttaacaat ttgcacgttc ccgtcgctca tcaagttttt cgcatcagat cccatgattt
     1261 attcctttgc tgcatctgtg tgcctttatt gctacctaag tgtaaaggct acggaggatt
     1321 tatccacgac agattttgag atggtggcaa acaactctgt ttaaactctg atacacagat
     1381 tattgggttg tgtcagatgt aaatgcgatc ctgaataaaa atcgcccttg caaatcaaca
     1441 aaatatagcc aatttgctta aaagctctgc aaacggtcga aatagcgcag aaaattacgt
     1501 tttgcctctt gccaccttcc cactctgccg ctaatattcg tccccgttgt cacctacaac
     1561 gttgcgttca tagctcagtt ggttagagca ccaccttgac atggtggggg tcgttggttc
     1621 gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt tggttagagc accaccttga
     1681 catggtgggg gtcggtggtt cgagtccact cggacgcacc agattttctt aatctggtct
     1741 tctccttttt ccctctgttt cttctctgta tccaatacat taaaagattt acactgtctt
     1801 cgtatgcgtt atcagaagga gaatcgctat ggcaactttg ttacaacttc attttgcttt
     1861 taatggccca tttggcgacg caatggctga gcagcttgaa ccgcttgctg agtcgattaa
     1921 tcaggaacct ggttttctgt ggaaggtatg gacagaaagt gaaaagaacc acgaagccgg
     1981 tgggatctac cttttcactg atgaaaaaag cgcccttgcc tatctggaaa aacatactgc
     2041 ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa gttttcgatg tcaatgagcc
     2101 actgagtcaa atcaatcagg caaaactcgc ctgacagatt ttaaccaagg gcggttagcg
     2161 cccttttcat ccctgtctga aatttctcaa attctaaaaa tttcaaccaa acttatctga
     2221 taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa tgaaaaaaat tgctattgtg
     2281 ggtgccgggc ctacggggat ctacacctta ttctcgcttc tacagcaaca aactccactt
     2341 tctatttcta tcttcgagca ggctgacgaa gccggtgtcg ggatgccata cagtgatgag
     2401 gaaaactcaa aactgatgct ggcaaatatt gccagtattg aaataccgcc gatttattgt
     2461 acgtatctcg aatggctaca aaagcaagaa gccagccatc tccagcgtta tggcgttaaa
     2521 aaagaaacct tgcacgatcg tcagttttta ccgcgaattc tgctgggcga atatttccgc
     2581 gatcaatttt tacgattagt agaccaggca cgacagcaaa aatttgcggt ggctgtttat
     2641 gaatcatgcc aggttaccga tctgcaaatt acaaatgctg gcgtcatgct cgctacaaat
     2701 caggatttac ccagcgagac gtttgattta gcggtgattg ccacgggtca cgtctggcct
     2761 gatgaagaag aagcaaccag aacgtacttt cccagcccgt ggtcaggcct gatggaagca
     2821 aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct tgagcggact ggatgcggca
     2881 atggcagtgg ctattcagca tggttcgttc attgaagatg ataaacaaca cgtcattttt
     2941 caccgcgata acgcaagtga aaagctaaat attacgttaa tgtcgcgcac gggtatttta
     3001 cccgaagccg atttctattg ccctattccc tacgagccct tacacatcgt cactgatcaa
     3061 gcattaaatg ctgagattca aaaaggcgaa tatggccttt tggatcgggt atttagattg
     3121 atagtagagg aaatcaagtt tgctgatcca ggctggagcc aacgcatagc cttagagagc
     3181 ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc gcaaacaacg cgacccattt
     3241 gactgggcag aaaaaaatct ccaggaagtc gaacgcaata aacgagaaaa acatactgtt
     3301 ccctggcgtt atgtcattct gcgcctgcat gaagcagtac aggaaattgt tccacatctg
     3361 aatgaacacg accataaacg gttcagtaaa ggccttgccc gggttttcat cgataattat
     3421 gcggcaatcc cttcagagtc tattcgtcga ctactggcct tacgtgaagc gggaatcatt
     3481 catattctcg ccctcggtga agactacgaa atggaaatta atgagtcgcg caccgtcctg
     3541 aaaacggaag acaacagcta ctcgtttgac gtttttattg atgcccgtgg gcagcgtccg
     3601 cttaaagtga aagatattcc tttccctgga ctacgcgaac aattacagaa aacaggggat
     3661 aaaaaccctg atgttggcga agattatacg ttacagcaac ccgaagatat tcgtgggcgc
     3721 gtagcgttcg gcgcgttgcc ctggttaatg cacgaccagc ctttcgttca gggacttacg
     3781 gcatgtgcag aaattggtga ggcgatggct cgggctgtcg taaaacctgc ctcccgtgca
     3841 cgtaggcgtc tttcgtttga ttaaaggtaa agctatcagc actcaacaaa ctgatgctgt
     3901 tcattaagac gatagcggac tccggctcga atattgtttt caccttcaat ggcgacggcg
     3961 aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc ttccccctgg cccgagaata
     4021 attgagtcga ccacgcccgt gctcactatt gttgaatctt caccgctggc aatgatgtgt
     4081 acattgtcgc cgctgttggc aattctggcg tttgcgccaa agctggcgat ttgcaccagg
     4141 tcaccattac tggcgacatg acaccgttca cccaatgtac aaacgcgtac tcgcattccg
     4201 gtattcgcaa tccgactact atctccagcg ctgcttatcc gcgaagaatt accggttaca
     4261 gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt taaagccgac acttccaatc
     4321 cgcacgctat aacccatact ggcaatttgc gcggcatacc cagcggttgc gattctggca
     4381 ttatcctcac aacagccgat gcgagcatga tcgtcgctgc gggcaaactg actgcaaaat
     4441 ggtctctctc cgggatctgt ggccaatttt tgcatagagg aaacttcctg atgcgcgaag
     4501 ttttcatccg ccagccattt tttccacgca tactcaacca gactctccgc ccagtccgta
     4561 tatccttcct caatcagtgc atgatgaata tcggcataac taccgccgcg tgggaaatgg
     4621 cgcaggaacc agcgatacat caccgctccg atacgccatt ctcgcaggtc tgctcgtgta
     4681 atgatcattc gttagtgccg gtgatagcca tcgaccatgc ttttaaaggt ttcgtgtggc
     4741 gtacgccccg tggtgcaaag ataaagatga ccgaagataa aaaagaggct tataaatgcc
     4801 agagcaaaat gcgcctgcaa taaccagtat cttacgccag gaaacacatc tcccacggct
     4861 tgcggataga gacacagcag ccccgtcaat agtaacaacg gcagcaatcc atacatgaca
     4921 ccaatatagg caacctgctg taagggatta aatttagact gtgttgttgc cgggaaagga
     4981 tgttcttccc cctgcataat gccaaacaaa tagaatcgcg tttgttttgc cgctcgctcc
     5041 agccacccct gacgacgaat gcgataatgg tgaccattac ccccaacggc attgatcaga
     5101 acaaagccga gccaacatgc cagtaacaaa aatccgcaaa cttcatgcac cgcaaccaga
     5161 cttttaaccg cagttgcgcc caccagcgca aagtgattta tcagcccact tgccagcaac
     5221 aatacaaaga gtaacgcatt cgaccagtgc cataatctga ccgctttgct gtaaagataa
     5281 accttctcac catggtcggt ggctgatttc ttcacgccac gagcacgaag caaggcatgc
     5341 agccccagca caaaccacat ccctaccagc aatagtccgg caatgatcaa ccacaccggc
     5401 caaaattggg gagtgaactg tggcacatag ttcgccaact ggctctgaaa ctgttcagca
     5461 tgttgcgacg ggttcataca ttttcctttt taatcaaatg ttgaccgaac cggcgataca
     5521 ggtgcggttt cccggcacca ggtagctgat attgatagta tttattttcc tgcaaccacg
     5581 cctgaatttc tgggctatct tcgcgaccaa agatcaatgc gtgttccgga caggcgctga
     5641 cgcaaatggg cggaaagcct tttgccagtc gggactcagc gcagaaatca catttatctg
     5701 ccactttagt gacagggtta aggtagcgaa cctggtaagg acaagcgcca atgcaataac
     5761 tgcagccgat gcactgcgat ttttctaccc gcacgatccc ctgctcatca cgccacgacg
     5821 cccctgtcgg gcatacgtcg atgcaaggag catcttcaca gtgctggcat gactggcgaa
     5881 aatagtgata ctgcgtttcg ttgtcgttat cggtgactgg aatgtgtgct atcgataaac
     5941 ggcttccctg agcggggacg tggttagttt tacggcatgc gcgggcgcaa atattacagc
     6001 cattgcaccg tgactcgtca tgaatcatgg cataccgaac tttcttttct tgcctcgtgt
     6061 tcgctaacag agaagacgca gaaccagtaa aaaagattac tgttcccatc cccagaacaa
     6121 atttgcgtcg agtgaaggac atctgattat cccctaatgc agtggctgca actgtccacc
     6181 ctatccatga catctccctg aaatttcagg ggttctgaaa ctgctctacc tcgaaccatt
     6241 cgcataagcc atacgccttg ccataggtca taaatcgctc gctaaacaag tacaacaccg
     6301 ccgtcgatga ctccacctgc cgtatatcag catattcagg tgcaatatcc atcgcggcga
     6361 tcgcggattc gatctgcgct tcctggaagt aatatggcgc ttgcatcagc atggcgactt
     6421 tgtaaggacg aggatacgtc tggcactcaa aacgaacggc gtgtgcaatg gcgcgacaaa
     6481 tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt ttcactcatg gcctgagttg
     6541 agtaatagta atcatcctgg cttcccgtca aacaagcgat atccgcgtgg tctccgttct
     6601 gcttaatgct ttccagcaaa actgacagtt cctcttctgc aaccgaatag ggtggcgaaa
     6661 gaaacacggc gcgcgccacc agctgcccag ccgcagaatg ctgacgaatg aactctgcca
     6721 ggatttcagc aggttctggt tcggttaccg tatcaacggg gtgacgcatc ccctgcaacc
     6781 attgccgctt cgcctcatca acttcgctga ctttcgctaa cggcaattca tcctgatggt
     6841 tcatctcacc cattccttac gcaggcagta gattgtgtgc tgccagatcg gcggcaatat
     6901 cttccagccc cagacgctgc aatgtttcac gggtcggaca accaagctgt gggtcccagc
     6961 ccatttcttt gtagaacatc gtgagcgaag catgcatatc gtcacggtcc attttgtcag
     7021 taccttcagt aaagaccggg atctgcggat ccttgtcgaa tacccaggaa cagataagat
     7081 cgtgttcatt acgcatatct ttggtttgca tcagttttac cgtgtaggca cgatgcaacg
     7141 taaagatacg ctctgcagct aaatctaatt tttcctgagt catctcttcg ccagtgatcg
     7201 ctttgaagaa tttggcttca agcgccagat cgccccgata attacggctt ttcagcggcg
     7261 aaacggtcat tggccagacc cagttgcaca gtgtgacggc gttatgcaaa cagacccgca
     7321 acagcgacca cttagcatat ttaatttttg cgtcattgat tggcgtgtag tttttggttt
     7381 catcgtaagc atcttcagaa ccaaaaagct ctttcgccac ctcacgttgc agtttcaatg
     7441 gcaaaccgga accaataaaa ttgatatggg tatgcgtcat gcagtcacgg ttgaacatac
     7501 agttaacaat ggaaccgact tgcgctgacg cttcattggc atggtgaact ggatagccaa
     7561 acggcgacca gagtttattt ttcgcgtagc cccagtactc ttcacccaaa ttccagcgtt
     7621 ctgcgatggc atatgaacca tcagccaggt gactcagctc acccacacga tgcgccagac
     7681 ggtagtaaaa atctttaatg aagttaacgt cacccgcttc cagttgatcc cagcgaattt
     7741 ccgcatactc ttcagctggt agaacacgct tgaacacccc tttgctgtaa caataagtaa
     7801 agtcgcgatg cagctgccca tagttacacc ataggccgta gtcatcgaat agattcagac
     7861 caaccaggtt accaatcaca cgaccatcgt ctttatcttc aaaatctttc ggcccgtttg
     7921 ggaagatggt ggtatggaca aagtttgcta cacaagtgtt accaccggtg ccaggcacgc
     7981 caaactcttt cacccgagga atattcattt gggtcataca acggatcggg caagagtggc
     8041 agccgctcat ttttactgtg tatttctctg ccgccggacc taagtcaaaa acggatttat
     8101 aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat ttcccccgtt tcaatcggac
     8161 caccttcagc cgcgccccaa aacagccctt tacgtgctgt ccaacgtgac ttgggatctg
     8221 aatactctgc ccacgattgc ggcgtacttg gcacgacatg gttgttattc gcaccgataa
     8281 gttcagtcat catgtaatcg ttgagacgct tcatctcctg acgatcggca atgttgaccc
     8341 ctttcgtccc ttcaaccgca atcgctttca ggtttttcga acccattatt gcgccagttc
     8401 ccgcaccacc gctgtggtta cggctattca acatgccaga gagaggaaca aggttttccc
     8461 cagcctgacc aatagccgcc acacaggttt cagggctggt caagcgacaa atctcttccg
     8521 tagttgcgcg cgtacctttt ccccataaga aatcggcttt ttccaggcta actttgtcat
     8581 ctttaatatt cagccatacc ggtgatttcg ctttcccttc gataataatg acgtcgtatc
     8641 cagcgaattt catttgcgct gcaaaaaagc cccccatatg ggcatcgacg actaaatttc
     8701 ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga actacagggg gcaccagatc
     8761 cagttaatgg gccggtagca aagactaatt tattcgcttc atcgaaaggt ttcgtgcctg
     8821 gcggtacttc gtcatacata attttgtagc cgaagcccat accaccaaca aaacttttaa
     8881 acttactgga atcttcgagg gtaatatttc ctgtcgtgag attgactctt aatatattac
     8941 ctgtccaacc gttagccatg attttttcct ttgcaagatt cacacagtaa tatctttcca
     9001 ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg cattcaccgc ataacacaca
     9061 tttcgaggat tttttacttt cggtatttac ggtggccatc atccacgggc atgccgtagt
     9121 acaggcgcta cagccaatac agcgtttatg atcgacggta atacagcctt ctttctgctg
     9181 ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc ggctctttgc attgtcggca
     9241 ggtgtccgcg gtatagttga gatcgccata caggccgccg ccagagccaa ccccgttgtc
     9301 gccaaagaaa taattgcgat ggattttgat acgggagaag aatgtcccta ctgagccatc
     9361 gttgaagttg gtacaagaga tttcacagcg gtggcaaccc gtgcatcgcg ctcgctgggt
     9421 caccagcacc cctttgggtg tatttatcaa ccctactgtg ccgctatcaa tatcttcctg
     9481 tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt agaccagcaa ggccttttcc
     9541 tgatatacgc aagaattcta atcgcgttaa gccaatatct aatagtggac gatcaaccgg
     9601 gttcatttat ttcgcatcct cttcgcaaac acagtgctga caattcagca gcctgatatt
     9661 aagcattcaa ctaatacgct gctgactctg tccccaacac cagatatttt ccctaacccg
     9721 atagtgtatg agaaaagatt taacgccgat aaactaatcc tttatggtta ggagaaaaaa
     9781 taa
//
LOCUS       DAOTRK010000163         9609 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.164, whole genome shotgun sequence.
ACCESSION   DAOTRK010000163 DAOTRK010000000
VERSION     DAOTRK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.164"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(467..574)
                     /locus_tag="QIE41_004021"
     CDS             complement(467..574)
                     /locus_tag="QIE41_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HDS3532843.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(979..2079)
                     /gene="chaA"
                     /locus_tag="QIE41_004022"
     CDS             complement(979..2079)
                     /gene="chaA"
                     /locus_tag="QIE41_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDS3532844.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIGINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            2349..2579
                     /gene="chaB"
                     /locus_tag="QIE41_004023"
     CDS             2349..2579
                     /gene="chaB"
                     /locus_tag="QIE41_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDS3532845.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2737..3432
                     /gene="chaC"
                     /locus_tag="QIE41_004024"
     CDS             2737..3432
                     /gene="chaC"
                     /locus_tag="QIE41_004024"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDS3532846.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(3476..3829)
                     /gene="ychN"
                     /locus_tag="QIE41_004025"
     CDS             complement(3476..3829)
                     /gene="ychN"
                     /locus_tag="QIE41_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDS3532847.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            4015..5409
                     /gene="ychO"
                     /locus_tag="QIE41_004026"
     CDS             4015..5409
                     /gene="ychO"
                     /locus_tag="QIE41_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDS3532848.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(5410..6060)
                     /gene="narL"
                     /locus_tag="QIE41_004027"
     CDS             complement(5410..6060)
                     /gene="narL"
                     /locus_tag="QIE41_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDS3532849.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDISVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(6053..7849)
                     /gene="narX"
                     /locus_tag="QIE41_004028"
     CDS             complement(6053..7849)
                     /gene="narX"
                     /locus_tag="QIE41_004028"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDS3532850.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAEGDGQLAQLQGL
                     QDYWRNELIPTLMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVLVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            8188..9579
                     /gene="narK"
                     /locus_tag="QIE41_004029"
     CDS             8188..9579
                     /gene="narK"
                     /locus_tag="QIE41_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDS3532851.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2322 a         2352 c         2480 g         2455 t
ORIGIN      
        1 gtcgtgccag aaagtcatgg caaactgcgt gagcgtcata tagccgcctt gttgtaatga
       61 caacattttg cggctattct tgaattgttc tggttcaaga ttagcccccg ttctgttgtc
      121 aggttatacc tctcaacgtg cgggggtttt ctctttccag caaccaatgc caccagggat
      181 agagcccccg caacattgcg cctcaccgga taacgccggc ttggtgtgga tactacgact
      241 caattcatct tcacttcatc cctgaaatgt atgcaatgaa gagtgcattc cggtttttca
      301 acagctgtta cagtcgtttc atgagtgctc tggatgatgc ttccagctcg ggttgccaat
      361 atttacttgt ggaagagata aagacaaaaa aggccgcagg ctgttacccc tgcggccggt
      421 ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcaccttac ttccggttac
      481 gccaccagct gacaatcgct gcggtaataa ttcccgccag gatcggtgct gccaggtcgt
      541 gccagaaaat catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca
      601 ttttgcggct attcttgaag tgtctggttt caagattagc ccccgttttg ttgtcaggtt
      661 ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc
      721 ccccgcaaca ttgcgcctca ccggataacg ccggcttggt gtggatacta cgtctcaatt
      781 catcttcact tcatccctga aatgtttgca ataaagagta cattccggct tttcaacagc
      841 tgttgcagtg gtttcgtgca ggttctggta caggctcgca attctgatta acaacctgtt
      901 caggtagtat gaagaaatac aataagcagg aatttatatt atctcccccg atttctgtca
      961 tcggaggaga tataactgtc aggcaaatat cgtcatcaaa taggcggcaa acagtgccag
     1021 atgcgctgcg ccattgagca cgttagtacg tccggtggag aaggagatat ggcacagcac
     1081 taaagaggcc accatcacta ccatttctgg cgcaccaagt gcaaactgca attcgttacc
     1141 cgtcataaag gcaattagcg tgacgacagg tacggtaagc gaaatggttg ctaacactga
     1201 accaaagaac agattcatcg cgcgctggac ctggttgttc aacactgctt ttaacgcacc
     1261 taaaccttcc ggcgacagaa tcaacagagc caccaggaag ccagtaaagg cgacaggggc
     1321 attcatgctg tcgagcaatg tctccagcgg gctggcgttc attttggtga ctgcaataac
     1381 ggcaatcaga tggacaatca accagatagc atgccaaagg ctgctatggg cagacggttt
     1441 accgtggtgc gggtcgtcat catcactgtc atcttcgtgc tcgtagacaa ataaactttg
     1501 atgcgttttg gtctggatca gcaaaaatac gccatacatt gccgcagaaa ttaatgctac
     1561 cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga gccattggaa ataccagtac
     1621 gattatcgcc agggggaaca gcgcaattaa atactgcttg ataccaaaca gattcatata
     1681 ttgggtggca aacttgcgtc cgcccaacaa taatgaaaag ccaaccagcc caccggtaac
     1741 aatcataatg attgaataga gcgtatcacg cattagcgtt ggcgcggcgt cgccggttgc
     1801 cattaaagct gaaatcaaac tgacttcaag aataaccact gaaaggctaa gaataagcga
     1861 accgtaaggt tctcccaggc gatgggctaa tacgtccgca tgacggacaa cactaaaggc
     1921 gctacttaaa ataccaataa gagcaagaag attgatgcca atgaccactg gtagtgtctg
     1981 gctgcttccc cacaggaaca gcactaccag cgccagaacc gggaaaataa gcgaagtctc
     2041 cttgtggcgg gtttttaccg cctcttgagc atttgacatt atggttatcc ctttgcagat
     2101 gaatttatcg aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc tggatatcgg
     2161 taacatttta cgaattttta ccccgctgtc gattttttac tcatcgggcc ataaaaataa
     2221 gcaatgcgtt tagacaatgt ttgttttagc gtttcttaag aagagtctga catgaaaatt
     2281 cttatgtttt ggcaagagtg gatattgttg accacactta atgttcaact ttgtaaaagg
     2341 agtcaacgat gccgtataaa acgaaaagcg atctgccgga aagcgtaaag cacgtgctac
     2401 cgtctcatgc tcaggatatc tataaagaag cgttcaacag cgcatgggat caatataaag
     2461 ataaagaaga tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa gtggcctggg
     2521 ctgctgtgaa gcatgaatat gccaaagggg atgatgataa atggcataaa aaatcgtaaa
     2581 accggtagct tagttaaagc aattcgtgcg gtgttgcctt gcaagtggtc cgtggattgc
     2641 atattgtccc gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga caccaaaaag
     2701 ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga taacgcgtga tttcttgatg
     2761 aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg gtcagcagag
     2821 caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg accggtgtgg
     2881 atcttcggtt atggatcgtt gatgtggaat ccggcactgg agtttaccga gtcgtgcacc
     2941 ggtacactgg ttggatggca tcgtgcgttt tgcctgcgtc tgaccgccgg gcgcgggact
     3001 gcgcaccagc cgggacgaat gcttgcactg aaagagggcg gacgcaccac aggcgtcgcc
     3061 tatcgactgc cagaagagac gctggagcag gaactaaccc tgttgtggaa gcgcgagatg
     3121 attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg acgcacagta
     3181 aacgccattg tgtttattat ggacccgcga catccagaat atgaatctga cactcgcgct
     3241 caggtcatcg cgccgttgat tgcggcggcg agcggtccac tgggaaccaa cgcacaatac
     3301 ctgttttcac tggaacagga gcttatcaaa ctgggaatgc aggacgatgg gctgaatgat
     3361 ttgctggtat cggtaaaaaa actgctggcg gagaattatc cggatggtgt gttacggccg
     3421 ggattcgcct gagtaaactt ccctcatagt ggggcgtcag acgccccctc aaacactaaa
     3481 atgtgagcac tttatcggct gacagcgtcc attgcgccag ttccacaagc gtaccgattt
     3541 ccaccccatc aatcagagga agtgtgctaa tcccgcgtcc gtcggtacag gttttgcaca
     3601 atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgaatg ttgtagcctt
     3661 cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatcagg aacaggcgca
     3721 gatccagatt gctctcctgc tctcgtaacg caatggccag ccgcaagctg ttaaacaagg
     3781 attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt atttactcct
     3841 gtattcaggg aattagacac tcatcttcta tcttactgct tctgcagcgt ctgaccaatc
     3901 ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag ttgccagtaa
     3961 tcgttattct ttagggctat ggtttttcat tttttaccgg aagttaccga cgttttgagc
     4021 cgtttcgttc ctcgcattat tccgttttat ttactcttgc tggcggcagg cggtacagct
     4081 aacgcacaat ctaccttcga gcaaaaagcg gcaaatccct ttgataataa caatgatggt
     4141 ctgccggatt taggcatggc acctgaaaat catgatgggg aaaaacactt tgctgaaatt
     4201 gtgaaagatt tcggcgaaac cagtatgaat gataacgggc tggatactgg cgagcaggca
     4261 aaagctttcg cattgggaaa agtccgcgat gcgcttagtc aacaggttaa tcagcacgta
     4321 gagtcctggc tatcaccgtg gggaaatgcc agtgttgacg tcaaagtgga taacgaagga
     4381 catttcaccg gcagtcgtgg aagctggttt gtgccgttac aagataatga tcgttatctc
     4441 acctggagcc agcttggtct tactcagcag gatgatgggc tggtgagcaa tgtgggcgtt
     4501 gggcaacgct gggcgcgcgg cagctggctg gtgggttata acacttttta tgacaacttg
     4561 ctggacgaaa atcttcagcg agcgggcttc ggtgccgaag cgtggggcga atatttgcga
     4621 ctatcggcaa acttttatca gccgtttgct gcatggcatg aacagacagc cacgcaggaa
     4681 caacggatgg cgcgcgggta cgacctgaca gcccggatgc gcatgccgtt ttatcaacac
     4741 ctcaatacca gtgtcagcgt agaacagtat tttggtgatc gtgttgattt gtttaactct
     4801 ggtacgggtt atcacaatcc cgtcgcgttg agtctgggat taaattatac cccagtgcca
     4861 ttagtcactg tgacggccca gcataaacag ggtgaaagtg gcgagaatca aaataacctc
     4921 gggctgaatc ttaattaccg ctttggtgta ccgctcaaaa aacaactttc tgcgggcgag
     4981 gttgccgaaa gtcagtcgtt acgtggtagt cgctatgaca atccgcagcg aaataatctt
     5041 ccgactcttg agtaccgaca gcgaaaaacg ttaacggtgt ttctggcgac accgccgtgg
     5101 gatctaaaac ctggcgaaac agtgccgctg aaattacaaa tccgcagtcg ttatggtatt
     5161 cggcaactga tttggcaggg cgatacgcag atattaagtt taacgccagg cgcacaagcc
     5221 aacagcgcgg agggctggac gctgatcatg cctgactggc aaaacgggga aggggcaagc
     5281 aatcactggc gattgtcggt ggtggtggaa gataaccagg ggcagcgtgt ctcctccaat
     5341 gagatcacgc taacgcttgt cgaaccgttc gacgcattgt caaacgacga actgcgctgg
     5401 gaaccgtaat cagaaaatgc gctcctgatg cacccatacc gctgcttcca cgcgagactt
     5461 gagcttcatt ttcttcagca tgtgcttgac gtgcactttt actgtgcttt cggtgatgtc
     5521 caggcggcgg gcaatcatct tgttcggcaa accctgggca atcagcttga gaatatcgcg
     5581 ctcgcgtggg gttaactggt taacatcgcg ctcagtagtg gcacggttag cgcgcaagct
     5641 ggcggccaga acaggcgtta atgcttcgct taataccatt tcgccagcag ctgcctgatg
     5701 caatgctttc agcagatctt ccggttccat atcttttaac agatagccat ccgcgccgcg
     5761 tttcagtgcg gtgaccacat cttcttcatg gttagagacg ctgaatacca caatgcgccc
     5821 tgagagggac ttttcgcgga gtttatccag cgtttccaga ccattcatgc cgggcatatt
     5881 gagatctaac aggatcagat cgggatcaag agattccgcc agttcaatac cctgttcgcc
     5941 attactcgct tcgccaacca cgctgatatc tggtgccata ctgataagct gttttacgcc
     6001 agttcgcagc atcgggtgat cgtcgatcag caggatagta gccggttcct gattactcat
     6061 gggtatctcc ttggacgtct gtgaaagttt tttcgggaat aaaggtgaca accacttcgg
     6121 tgccacctga ttcacgacgg cggacgcggc aatcgcctcg taaactttgc gcacggtcgc
     6181 gcattattat catgccgtag tgattgctgc ggatggcatt ttcaggcacg ccgcagccgt
     6241 tatcctggac ggtcagtttg acctgattat cgttttgcgc caccgtcacc aagacctcac
     6301 tcgcttgcga atgtttgagg gcgttactta atgcctcacg ggcaatttgc aacaagtgga
     6361 ttgcctgatg cgaaggcacc agacgaggtg gcaattgata atccagcttc accgggaagc
     6421 caaatttggc gctgtactct tcgcaactcg cctccagcgc tggacgtaat ccaggctcgg
     6481 tgagctgcaa gcggaatgtg gtgagcaatt cacgcaactg cgcccaggat gcattcagtt
     6541 cgttacggat ctgacttaac agttcgcggc tgctttctgg cagcgcatcg ccctgcatct
     6601 gtaaacaact cacctgcatc ttcatgcaag agagagattg ggcaatagaa tcatgcagtt
     6661 cgcgcgcaat ggtggcacgc tcttccatca cgatcaactg ttgctgacgt tcctgatggc
     6721 gatccaacgc cagcgtggcg gtgagttgtt caaccagggt atccaccagt tgttgttgat
     6781 catggctaag atgacgcccc tgcggtaggg tcgccagcaa aatgccgtac tgcgtatgag
     6841 agtcagccag ccgccacttc agggttgtgc cgcgatcgcc aacgggtaat acgccgcgcg
     6901 ggcagagctg gcagccttta tcatcacaag tcatatctgg ctggcaggta aactcctgat
     6961 gattctcttc atcatcagtg tcatacaccc gcagttcgat atcacgtagc agggtcaaat
     7021 tctgtaagcc gttgagtaca ggtgacaggc gttcacacag cggggcgcgg gaatgcaaac
     7081 ggcggttagc ctgccataaa aaagagagga tctgattttt atgctccagc ccggcagttt
     7141 tctcctgaac ccgctgctca agtacggcat aactttcggc cagttctgca gacatattgt
     7201 tcaatgcagt tccaagcatc gccatttcgt tgcgcccgct gatgtttgcg cgttgggtaa
     7261 aatcgcgatg gctgacggca ctcgccattg ccagcagttg ccgccacggt tgtagcaatc
     7321 gcgcccgcaa ccagataata gtgaacacca gtaaaagtgc cataaatacc gccattaccc
     7381 gatggaccag caccactgtc tcaatgcgca tttccgtggt gcggtcaaaa ccagatacca
     7441 gctgatcaag cccggcaaca aactggctga catccgctga caccgtttcg cggttttgtg
     7501 cacgcatcag cgtagggatc agttcattac gccagtaatc ttgtaaaccc tgtaattgcg
     7561 ccagttgtcc gtctccttct gctgctcggg ttaactcggc gctaaatgcc gtttgttcca
     7621 tttctttaat taagggcttg tctttctcgc ttaatggcac tgctgccaac agacggtaac
     7681 tttgcatgcg cagcgatccc gctttgttga tcgcatgggc gctgccctga acgccttgca
     7741 ccagccagcc agaaaccgcc atccctgcca gtccaatagc agtagaaagc aacacaataa
     7801 gcgcaacctg attaaccagg gtgagcggag agagacaacg tttaagcatg taaacctctt
     7861 ccttcaggct ttaaatgagc aataacctta atgaatgtga cgatacattc tggaatggca
     7921 gtattctcgg ctattggctg aagtataccc atacccggaa agagttactc cttatttgcc
     7981 gtgtggttag tcgctttaca tcggtaaggg tagggatttt acagcaccgt gaaaaatctc
     8041 ataattttta tgaaatcact gtactcacta tgggtaatga taaatatcaa tgatagataa
     8101 agttatctta tcgtttgatt tacatcaaat tgcctttagc tacagacact aaggtggcag
     8161 acatcgaaac gagtatcaga ggtgtctatg agtcactcat ccgcccccga aagggctact
     8221 ggagctgtca ttacagattg gcgaccggaa gatcctgcgt tctggcaaca acgcggtcaa
     8281 cgtattgcca gccgcaacct gtggatttcc gttccctgtc tgctgctggc gttttgcgta
     8341 tggatgttgt tcagcgctgt tgcggtgaac ttaccaaaag ttggttttaa ttttacgacc
     8401 gatcagctat ttatgttgac tgcgctgcct tcggtttctg gcgcgttatt acgtgttcca
     8461 tactccttta tggttcctat cttcggtggt cgtcgctgga cggcgttcag caccggtatt
     8521 ctgattattc cttgcgtctg gctgggtttt gccgtgcagg atacctccac gccttatagc
     8581 gtcttcatca tcatctctct gctgtgcggc tttgctggcg cgaacttcgc ttccagtatg
     8641 gcaaacatca gcttcttctt tccgaaacag aagcagggtg gcgcgctggg tctgaatggt
     8701 ggtctgggca acatgggcgt cagcgtcatg cagttggttg ctccgctggt ggtatcactg
     8761 tcgattttcg cagtatttgg tagccagggt gtcaaacagc cggatgggac tgagctgtat
     8821 ctggcgaatg cgtcctgggt atgggtgccg ttccttgcca tcttcaccat tgcggcgtgg
     8881 tttggcatga acgatcttgc tacctcgaaa gcctccatca aggagcagtt gccggtactc
     8941 aaacggggtc atctgtggat tatgagcctg ctgtatctgg caaccttcgg ctccttcatc
     9001 ggcttctccg cgggctttgc gatgctgtca aaaacgcagt tcccggatgt tcagattctg
     9061 caatacgctt tcttcgggcc gtttattggt gcgctggcgc gttctgcagg tggtgcatta
     9121 tctgaccgtc tgggcggaac tcgtgtcaca ctggtgaact ttatcctgat ggcgattttc
     9181 agcggcctgc tgttcctgac cttaccgact gacgggcagg gcggaagctt catggcgttc
     9241 ttcgcggtct tcctggcgct gttcctgaca gctgggctgg gtagtggttc cactttccag
     9301 atgatttccg tgatcttccg taaactgaca atggatcgcg tgaaagcaga agggggttct
     9361 gacgaacgtg cgatgcgtga agcggcaacc gacacggcgg cggcgctggg tttcatctct
     9421 gcgattggcg cgattggtgg cttctttatc ccgaaagcgt tcggtagctc gctggcatta
     9481 acgggttcgc cagtcggcgc aatgaaagta tttttgattt tctatatcgc ctgtgtagtg
     9541 attacctggg cggtatatgg tcggcattct aaaaaataaa tcttaatatt tgattatcct
     9601 ttgcgcggc
//
LOCUS       DAOTRK010000164         9562 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.165, whole genome shotgun sequence.
ACCESSION   DAOTRK010000164 DAOTRK010000000
VERSION     DAOTRK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.165"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(91..837)
                     /locus_tag="QIE41_004030"
     CDS             complement(91..837)
                     /locus_tag="QIE41_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDS3532852.1"
                     /translation="MKKVIVVSALAMAGVFSAQALADRGKTGFYVTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSHY
                     TLDTWHSPMGDGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKAT
                     YTDTSWNKSGEVSDISALHYSGYDNNFAWSIGAGVRYDITPDIALDLSYRYLDAGKSS
                     LSYKDAEGDKYKSEADVKSRDIMFGVTYNF"
     gene            1015..1245
                     /locus_tag="QIE41_004031"
     CDS             1015..1245
                     /locus_tag="QIE41_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDS3532853.1"
                     /translation="MQRHGSIETRTARDKAAERQMSHMDVTQCRPCIIHKAEYLDKRK
                     LMLQWWVDFLDANRERAITPFDYAKINRGNGE"
     gene            1439..1732
                     /locus_tag="QIE41_004032"
                     /pseudo
     CDS             1439..1732
                     /locus_tag="QIE41_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1698..1811)
                     /locus_tag="QIE41_004033"
                     /pseudo
     CDS             complement(1698..1811)
                     /locus_tag="QIE41_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2434..3390
                     /locus_tag="QIE41_004034"
     CDS             2434..3390
                     /locus_tag="QIE41_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532854.1"
                     /translation="MAVSIAEYLGQRTDVDQQIVPVAKGTQIQCPFMDRTCDKASKVK
                     NPTPPVCSVRKPDGTVWIVCEHRLCSTRQKKTVIVNGRKKQIENILVEHQRDILRKVA
                     KLIYQDPELQDSEIGVRREVNIPLPDSDNSYHADYVMRNFSGRGRVDEVLLEMQGGGE
                     TSSTGEITRHIAAWADLEFPTNEILRQPVAANTIETNAWRRQQEQFLVKGNVVDQTGG
                     KIVFAVGSLLYDYLHRRFRNANLRDLKAHNWTLCILAFKEDTSDEPRPGPIPLIIDDS
                     KTLFTNYSTFVRFLTDQGAPRPELFEGSFLRLDNSSVTIMPR"
     gene            complement(3371..4300)
                     /gene="dcm"
                     /locus_tag="QIE41_004035"
     CDS             complement(3371..4300)
                     /gene="dcm"
                     /locus_tag="QIE41_004035"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDS3532855.1"
                     /translation="MKKKPLKQYKVSSFFAGIGGFDLGLEKAGMEVVFQCEINKFCQK
                     VLRKNWSKVPLHTDITRLNADEIPESNVWCGGFPCQDVSSANQGKRKGLEGARSGLFY
                     TYAKLIEERKPEWLIIENVPGLLNSHNGQDFKVVIDTLVEFGYGVSWRVLDAKYFGTP
                     QRRRRVYIVASLGDMRSARVLFEPGATRIVDKQGQGERANASGASATGLSKADIYSIQ
                     HASIGRKATAGPQAKGYRNDGETYTLDSRGSSDAVCAPDAAFRVRASTGISRELDSNR
                     FRAVGNAVAVPIVEWLGKRIVLVDQQVLNEALS"
     gene            complement(4555..4657)
                     /locus_tag="QIE41_004036"
                     /pseudo
     CDS             complement(4555..4657)
                     /locus_tag="QIE41_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705337.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4754..4852
                     /locus_tag="QIE41_004037"
     CDS             4754..4852
                     /locus_tag="QIE41_004037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS3532856.1"
                     /translation="MKKRFSDEQIISILLEAKAGVSARELCRALDQ"
     gene            4832..5128
                     /locus_tag="QIE41_004038"
                     /pseudo
     CDS             4832..5128
                     /locus_tag="QIE41_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5436..5552
                     /locus_tag="QIE41_004039"
                     /pseudo
     CDS             5436..5552
                     /locus_tag="QIE41_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
     gene            5554..5687
                     /locus_tag="QIE41_004040"
                     /pseudo
     CDS             5554..5687
                     /locus_tag="QIE41_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
     gene            complement(5762..7213)
                     /gene="nhaC"
                     /locus_tag="QIE41_004041"
     CDS             complement(5762..7213)
                     /gene="nhaC"
                     /locus_tag="QIE41_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDS3532857.1"
                     /translation="MNNNNTPRSPTLVESLLPILAMILLLGFGYAAFDLPPEPLMVLS
                     TVIAALLVKRLGYGYNAILESISQKIAKTMPALLILISVGLLIGTWMIGGTIPLMIYY
                     GLNLINPSMIYVTALLVTAVVSVCTGTSWGSAGTVGVAFMGVAIGMEANLAATAGAIV
                     AGAYFGDKLSPLSGDTNLAAMAAQIDLYEHIAHLLYTTLPSLILSAFVMTLYGMNSEL
                     GGSTIPEKIQLITEGLKGIYHFNLLLLLPIAIVLYGSITKKPTIPIMLVSALVAMINA
                     SVIQGFALHDIVKSAVDGFNVSMLTDKDVNPLLGNLLNRGGMNSMMSTLLICFCALSF
                     AGTLALSGALEVIVHNLLKLVHSTGTMILATIACGLTMISVTCNGQISILIPIEMLRS
                     AYIERGLHPKNLARTVEDSATIFEPILPWTAAGAYMAGTLGVATLSYLPWAILCWSGI
                     FFATLWGFTGFGIARLSKEKQQQLMMETINESK"
     gene            complement(7206..8384)
                     /locus_tag="QIE41_004042"
     CDS             complement(7206..8384)
                     /locus_tag="QIE41_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDS3532858.1"
                     /translation="MKVYNFDKVISRDGTYSAKYNKKGRDIIPLSVADMDIPVADFIV
                     SELSVANQKGIYGYTLLSDDWQQVAAQWYQRHYSWKVNPEHIVFCPRVVQAVSLFIQN
                     FTQPGDAIVSLTPAYHPISHAVEVNHRVLLESALLYRDHHYEIDFADLEDKFKMACCF
                     ILISPHNPTGTIWSEDNLRKIASLAEKYNVFIISDDVHADFNFTQKQHQTISSISSYV
                     AQNSFICTSPAKTFNMAGLEVANIVIANDKYREQFKLALIAAGIHNPGYFSVPAFLCA
                     YRHGDSWLAALKDYLVENRSWVQSFCQAHFPDWFVASGGGTYMLWIDYRAMNISEEQL
                     RHWFVSLAGVEMSWGSDFGDEGLGFFRVNIATPRSNLTQAFERIYQSIPFTTGVSLHE
                     "
     gene            8858..9562
                     /locus_tag="QIE41_004043"
                     /pseudo
     CDS             8858..9562
                     /locus_tag="QIE41_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    9139..9255
                     /locus_tag="QIE41_004043"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         2737 a         2173 c         2140 g         2512 t
ORIGIN      
        1 attactgcag ggtatttctg tttagttgca gaagatatat aaatatctta attactgaat
       61 atgtgtaatt atcgctcagg agcagtgtcg tcagaagtta taagtcacac caaacatgat
      121 gtcgcgactt ttaacgtcag cctctgattt atatttatcc ccttctgcat ccttgtaaga
      181 cagggaggat ttaccagcat ccagatagcg atagctgagg tcaagagcga tatccggggt
      241 tatgtcatag cgaacaccgg ccccaatgct ccatgcgaaa ttgttatcat atcccgagta
      301 atgcaacgca gaaatatcgc tcacctcacc agatttattc caggaggtat cagtatatgt
      361 tgccttatga tgtatccgtg cataacccag tccaacagac acccatggag tgaatgcact
      421 gctgtttctg aagtcataat acgtattcac catcagggta ttcacactga gcctgttctg
      481 tgtgtcttcc cgaccaccat cccccatcgg agaatgccat gtatccagtg tataatggga
      541 gtctgcagct ccgcggccat agaattccac ttccgtgcgc acaggaacat tgtaatgttg
      601 ataaaaatca tagcccaccg caaggcctgc accaaataca gtatcgtttt tatcaccgcc
      661 cttatactta taaacatcat ccccaaagtc ctgacggaag cgctggtcag tctgagtcac
      721 aaccgaagca ccagctttac cggtcacata aaagcctgtt ttacccctat cagccagagc
      781 ctgggctgaa aatacgcccg ccatagcaag cgctgaaaca acaataacct ttttcatttc
      841 aatcatctcc gttggttctt acccgcacga taaagaaggt tgcttatttt aaaaccatgc
      901 aaaagacagg gtaaaaagag aaaattcgat cgaatcgatc gacaaaaccg atcatgcatg
      961 atgaagattt cttatcgaag acatgaaaat ggtaaggaat ggtttaagag gtaattgcaa
     1021 cggcatggaa gtattgagac cagaacagca cgagataaag cggctgaacg tcagatgagc
     1081 cacatggacg taactcagtg caggccgtgt ataatccata aagcggaata tctggataag
     1141 cgtaagctga tgttgcagtg gtgggtcgat tttctggatg ccaatcggga aagagcgatt
     1201 acgccgtttg actatgcgaa gatcaatcgg ggtaatggcg agtgagttga atgccgttac
     1261 tccttggctg atgtttaaat ttaaaagacc aaccagaccg cttaggactg gttggtcgct
     1321 gtatttcatc tgccaggtac agagccgtca ggcaaaaagg atgtctgcaa taatgccttg
     1381 aatatacata tagttgcgag catcatcgca aacatatgct catgaattct tcaacgcaat
     1441 gacctctgac aaaatcctaa agcaagcgat atcaaatatc accatctggc gcaaaggtga
     1501 acaacgtgcg ccacataagc cattattgct gctgtacgtt ctctcacact atcggcaggg
     1561 tcatggtcgc ctgtttaact atgcttctga aatctacgag cccctgttgg atctcctgga
     1621 acgttatggg ccgcagcgcc gtgaccagcg cccggacatg ccgttctggc gtctgaaggg
     1681 cgatggcttc tgggaactac attacgcgga actgtgctca accagtggta acactgagca
     1741 cgcagttcat ccgtcaggcg tggtgcaccg taacgctgtt ttgaccgggt aaaagccgcg
     1801 aggacaacgc tgtgcaggaa atcgtccgca tccccataac ggtcaaacat cgtggcgttg
     1861 acattgtgca tctgtgccag taaaccgcaa tttaactcct ctttcccgat ctccatacat
     1921 tcgctgccag ccaagctact gcggaacgcc actgttattg cctgattcag cagctatttg
     1981 gtatccgcgc gtagtcccag tgtcgtaagc ggctcgccag aactgagtgg tcagctcaac
     2041 ttttcagggt aacaggtcgc gcatcctgac gggagcaacc cgcaaaatgc cgccatacca
     2101 tctctgatac gctcatcagg gggagtattg ttctgacagc ggcaggtcca cagggacaaa
     2161 actgtaaatc acttcttcat aggaatgccc ttacgttgct gatgaaggca tggctaacac
     2221 cacggtgtat taatcaacgg ggagcaagtc agattggctt cactatcttg ggaaataatg
     2281 agtgcatcga gagatgagca gcgtcaagac tcggacttcc ttcattttca tttaaaactt
     2341 acacctaaga tatagaagtt ctatgaatta attgacataa attaccactc actatgatca
     2401 tatcgtataa gattcttgac tgaggaatga gcaatggcag tgagtatcgc ggaatattta
     2461 ggtcaaagaa cagatgtaga ccaacagatt gttcccgttg caaaaggtac gcaaatacaa
     2521 tgccctttta tggatcgaac ctgtgacaaa gcaagtaagg tcaaaaatcc tacacctcct
     2581 gtatgcagtg tccgcaaacc agatggaact gtttggatag tttgcgaaca tcggctatgt
     2641 tctactcgcc agaaaaagac agtcattgta aatggtcgta aaaaacaaat tgaaaatatt
     2701 ttggttgaac atcaaagaga tatattacgg aaagtagcaa aactcattta tcaggatcct
     2761 gaattacagg attcagaaat tggtgtaaga agagaagtca atattccttt accagattct
     2821 gataatagct accatgcaga ctatgtaatg agaaattttt cgggtagggg aagagttgat
     2881 gaggtcctgc ttgagatgca aggtggtgga gaaacaagta gcactggaga aattactcga
     2941 catatcgctg catgggctga tcttgagttt cctactaatg aaattctaag acaacctgta
     3001 gcagcaaata ctatcgaaac aaacgcatgg agacgacagc aagaacaatt tcttgtcaaa
     3061 gggaatgttg tcgatcaaac cggagggaaa atcgtttttg ctgtcggtag cttactctat
     3121 gactatttac acagacgttt taggaatgca aatctcagag atctgaaagc ccataactgg
     3181 acattatgta ttttagcttt caaggaagat acatcggatg aaccacgtcc agggccgatc
     3241 ccgttaatca ttgatgactc aaaaacacta ttcaccaatt attcaacatt tgtaagattt
     3301 ctaacagatc agggcgcacc acgccctgaa ctattcgaag gatcattttt acgtttagat
     3361 aatagttctg ttacgataat gcctcgttga gcacctgctg atctacaagg acaattcttt
     3421 ttcctaacca ttcaacaata gggaccgcga cagcgttccc tactgctctg aatcgattag
     3481 agtccagttc tcgggaaatc cctgtagacg ctcgtactcg gaaggcagca tccggcgcac
     3541 atacagcatc tgaacttcct cttgagtcaa gagtgtaggt ttctccatcg ttcctgtatc
     3601 cttttgcttg aggtcctgcg gttgcttttc gtccaatact tgcatgctgg atagaataga
     3661 tatctgcttt tgataggcct gtagcagagg ctccggaagc atttgccctc tctccttggc
     3721 cctgtttatc aacgattcta gttgctccgg gttcaaaaag gactcgagcg gagcgcatgt
     3781 ctccaagact tgcgacaatg tacactcttc ttctacgctg gggtgttcca aagtattttg
     3841 catccaatac tctccacgaa acgccatacc cgaattcgac caacgtatcg atgacaactt
     3901 tgaagtcttg tccattgtgg ctgttgagca gtccgggaac attttcaata ataagccatt
     3961 caggctttct ttcttcgatg agttttgcat atgtataaaa taagccgctt cgggcccctt
     4021 ctagaccttt ccgctttcct tggttagctg acgacacatc ctggcaagga aaaccaccac
     4081 accaaacgtt gctctctggg atctcatcag cattaagtcg tgtaatatca gtatgtagtg
     4141 gaacttttga ccaattcttt ctaagtactt tctggcaaaa tttgttgatt tcgcattgga
     4201 aaacaacttc catccccgct ttctcaagac caagatcgaa tccaccaatc cctgcgaaaa
     4261 acgaggatac cttgtactgt ttcagtggtt tctttttcat atcggctttt tcatcacttt
     4321 ttgaaccaag gatcctaacg gaaaccagcc ccgatggcca ccttttttct aattaaagcc
     4381 actttcttgg tatccaggca ttgattatga aataaactaa ctgtaattat atacagtata
     4441 ttttaaatgc caataaaagt gtacttcttt gcgaaaagcc ttctaatttg actagacttt
     4501 cgcatagtca aacgggctaa tccctttctc gcgacttaca tccagaaaat ccactcacca
     4561 ttgcaacatc agtcgctgca tctttcaaat gttccacttt atggatataa gcagcccgta
     4621 cttttttacg ttcctgatga ctcatctgac gtccaaccac tgacgtccaa ccactcgcgg
     4681 gactgacctg ctccctgttg attaatacac cgcgatgtta gtaatgtctt cataagctac
     4741 atgaggacat cccatgaaga agcgtttttc cgacgaacag atcatcagta tcctcctcga
     4801 agctaaagcc ggggtttctg cccgtgagct ctgccgcgca ttggatcaat gagcctttga
     4861 gcatagtgtc gagttgcgtt taatccagcc gggcaagcca acgcagaacg gatttattga
     4921 gagctttaac ggacgatttc gcgatgaatg cctgaatgag cactggttca gcgatatcgt
     4981 tcacgctagg aaagtgatta atgactggcg gcaggattat aacgagtgtc ggccacattc
     5041 agcactgaat tatcagacgc catcagagct tgcagcacta tggcgaaatg gaaaatgtga
     5101 aggtaaccaa accgacatta ctaactgata attgtatcta atactggggg caggtcagga
     5161 ttgtcaacga cggatgaaaa gtggttcttc cgcaacttgg gtggaagaaa atggatgaaa
     5221 ctttctggtg aacagtgaac cgccccggaa atcctggaga ctaaacttcc tgagaaagag
     5281 gtaaacagga tgactaaaaa aacacgtttt tcccccgaag tccgtcaacg ggcagttcaa
     5341 tgctttgtcg aataaacatt tacctgaacg attaactgca acggctatcc ttaccgacaa
     5401 tcaacgtcaa taaaaatctg tactgactcg tcagcactgg gttcgatcag taggaacatg
     5461 aattcaaagg ccaccttgct gaatctcaag actcctccga ttccaaagat aaaccccgtc
     5521 ttaagttcgc tggcctgaaa ttcggtgact attgtctata tcgggatcac cacggttgtg
     5581 tgattaatcc tcgtcgatca taaagcgtct atggatgtac tgcggcgttc ctgctactgg
     5641 tgatcaccct gtggttgtgc aattccaaac ggctgaagcg agagtaacag acatcacgcc
     5701 gcaatcgggg gcggcgtgta gacgtggtta attactatca aatatattaa tcaggccgat
     5761 tctatttgct ttcatttatg gtttccatca tgagctgttg ctgcttttct ttggacagtc
     5821 gggcgatacc aaagcctgtg aacccccaca gcgtcgcaaa gaaaatgcca ctccagcaga
     5881 gaatagccca cggaagatag cttaatgtag caacgccaag tgtacctgcc atgtaggcac
     5941 ccgcggcagt ccagggcaga attggttcaa atatggtggc agagtcttct acagtccttg
     6001 ccagattttt tggatgtagg ccgcgctcaa tataagcact tcgtagcatt tcaatcggaa
     6061 tcaggattga tatctgacca ttacaggtca cgctaatcat tgttaaacca caggcaatcg
     6121 tcgcaagaat catggttcct gtggaatgaa ctaatttgag cagattatgg acgatgactt
     6181 ctaaagcgcc gctcaaggct aatgttcccg caaatgaaag ggcgcagaaa caaataagca
     6241 acgtactcat catcgagttc atgccaccgc ggtttaacaa attacctaat agcggattaa
     6301 catctttatc ggtaagcatt gaaacgttaa atccgtctac tgcactttta acaatgtcgt
     6361 gtagtgcgaa cccctgaata acgctggcgt tgatcatggc taccagtgcc gaaacaagca
     6421 taattggaat ggttggtttc ttggtaatcg aaccgtacag gacaatggca atgggtagca
     6481 ataacaataa attaaaatga taaattcctt ttaatccttc agtgattagc tgaatttttt
     6541 cgggaatggt tgagccgccg agctcgctat tcatcccata gagcgtcata acaaaggctg
     6601 acagtatcaa tgaaggaagg gttgtataaa gtaaatgagc gatgtgttca tagagatcta
     6661 tctgggctgc cattgcggct aagttagtat cgccagaaag gggagacagt ttgtccccga
     6721 agtaagcccc tgccacaata gcgcctgcag ttgctgccag attagcctcc attccgatag
     6781 ccacacccat aaacgcaaca ccgacggtgc ctgcggatcc ccaggatgtc cctgtgcata
     6841 cagagacgac ggcagtaacg agcagggccg tcacatagat catggaagga tttatcagat
     6901 taaggccgta ataaatcatc agcggaatag taccgcctat catccatgtc ccaatcagga
     6961 ggcccacgct gatcaaaatt agcagagcag gcattgtttt agcgattttc tgcgatattg
     7021 attcaaggat tgcattgtag ccatacccca accgcttaac taacagtgca gcaatgacgg
     7081 ttgaaagaac cattaatggc tcaggtggca gatcgaatgc cgcataacca aatcccagca
     7141 atagtatcat cgctagtatt ggtaataatg attccaccag agttggggat cttggagtat
     7201 tgttattatt catgaaggga aactcctgta gtgaagggga tgctctgata aatccgttca
     7261 aatgcttgtg taagattgct tctcggcgtg gctatgttca cacggaaaaa accaagtcct
     7321 tcatcgccaa aatctgatcc ccagctcatc tcaacacctg cgagcgagac aaaccaatgg
     7381 cgtagttgct cttcgctgat gttcattgcc cggtagtcga tccacaacat gtaagttccg
     7441 ccaccagagg cgacaaacca gtctggaaaa tgagcttggc agaatgattg aacccagctc
     7501 ctgttttcga ccaaataatc ttttaatgct gctaaccaag aatcgccatg gcggtaagca
     7561 cagagaaatg caggaacaga gaaataacca ggattatgga tacctgctgc aataagagct
     7621 aatttaaact gttcacgata tttatcgtta gcaataacaa tatttgcgac ttcaagtccg
     7681 gccatattaa atgtttttgc tggagatgta caaataaacg agttttgtgc aacgtatgag
     7741 cttatagaag aaattgtttg atgttgtttt tgggtgaagt tgaagtctgc atgaacatca
     7801 tcagaaataa taaaaacatt atatttttct gctaatgaag ctattttcct tagattgtct
     7861 tctgaccaga ttgttcctgt tggattatgc ggcgatatta gaatgaaaca acaagccatt
     7921 ttaaacttat cttcgagatc cgcaaagtct atttcatagt ggtggtcacg ataaagtaac
     7981 gcactttcca gcaatactcg atggttgact tcaactgcat gactaatagg atgataggct
     8041 ggtgtcaggc tgacaatagc atctcccggt tgggtaaagt tctggataaa taatgagacg
     8101 gcctgtacta cccgtgggca aaaaacaatg tgttcagggt tgaccttcca cgagtaatgg
     8161 cgttgatacc actgtgctgc cacttgctgc cagtcatcac ttaataatgt ataaccgtag
     8221 atacctttct gatttgcgac agataattca gaaactataa aatcagctac aggaatatcc
     8281 atatcagcta cagacagagg aatgatgtca cgcccctttt tattatattt agcgctatag
     8341 gttccgtcgc gtgaaataac tttatcaaaa ttataaactt tcatatttgt tactcgtccg
     8401 attttatcgt tagggtaatg taatatttcg agggaggaag tgttattctg tattttgttt
     8461 tcgttattat gaaaatttgc atatgtgatg tttatcacaa agatgttaac tatctgtcaa
     8521 taaaaagact ccagcaaatg taaacgtttt acatcgctta agcctatgga atttacataa
     8581 aaaaaagggc gtgccgagtc tttcgaccca atcattatct cggtaactcc attcacaaga
     8641 gcaggaaata accccttcaa caaataacag actgatgcct gcgagcgcgc tccccaggtt
     8701 tccgggctga aagtaccgcc gcactcgttt aatgcggaac agtctgtgtt ggcggtttaa
     8761 tggtggatgt tgctgtagac cacaactgat gaggcaacgg agttgaaccg ccccggaaat
     8821 cctggagact aaacttcctg agaaagaggt aaacaggatg actaaaaaaa cacgtttttc
     8881 ccccgaagtc cgtcaacggg cagttcgtat ggttctggaa agtcagggcg aatatgactc
     8941 acaatgggcg gcaatttgtt ccattgctcc aaagactggc tgtacaccgg agactctgcg
     9001 tgtctgggta cgccagcatg agcgggatac cggaggcggt gatggcgggc tcaccaccgc
     9061 tgaacgtcag cgtctgaaag agctggaacg tgaaaatcgt gaactgcgcc gcagtaacga
     9121 tatccttcgc caggcttccg cttattttgc gaaggcggag ttcgaccgcc tctggaaaaa
     9181 gtgatgccac tgctggataa gctgcgtgag cagtacgggg tcggaccgct atgcagcgaa
     9241 ctgcatattg ccccgtcaac gtattaccac tgtcagcaac agcaacatca tcctgataaa
     9301 cgcagtgccc gtgcgcaggc tgaagaaaga gatacagcgc gtatacgatg aaaatcacaa
     9361 ggtatacggt gtgcgtaaag tctggcgtca gttgttacgg gaaggtatca gagtggccag
     9421 atgcactgtg gcacgtctca tggcggttat gggacttgcc ggtgttctcc ggggtaaaaa
     9481 ggtccgtacg accatcagcc ggaaagccgt tgccgcaggt gaccgcgtaa accgtcagtt
     9541 cgtggcagaa cgacctgacc ag
//
LOCUS       DAOTRK010000165         9523 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.166, whole genome shotgun sequence.
ACCESSION   DAOTRK010000165 DAOTRK010000000
VERSION     DAOTRK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.166"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1861)
                     /gene="espL1"
                     /locus_tag="QIE41_004044"
     CDS             complement(<1..1861)
                     /gene="espL1"
                     /locus_tag="QIE41_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HDS3532859.1"
                     /translation="MSENDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADMYVRNCAIAFLPL
                     LPEGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLIALFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPITQELLSKKMVMDILAMKTRDGEPGLYAAMENNHP
                     LCVTRFLSKVYGIAVKYNLSKINIMDLLKGATAYGTPALYIAMSKGNKDVVLSYISTL
                     GTFANKYSFSQCQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQS"
     gene            2384..4312
                     /gene="thrS"
                     /locus_tag="QIE41_004045"
     CDS             2384..4312
                     /gene="thrS"
                     /locus_tag="QIE41_004045"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDS3532860.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4316..4858
                     /gene="infC"
                     /locus_tag="QIE41_004046"
     CDS             4316..4858
                     /gene="infC"
                     /locus_tag="QIE41_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDS3532861.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4955..5152
                     /gene="rpmI"
                     /locus_tag="QIE41_004047"
     CDS             4955..5152
                     /gene="rpmI"
                     /locus_tag="QIE41_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDS3532862.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5206..5562
                     /gene="rplT"
                     /locus_tag="QIE41_004048"
     CDS             5206..5562
                     /gene="rplT"
                     /locus_tag="QIE41_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDS3532863.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5679..5803
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5685..5729
                     /gene="pheM"
                     /locus_tag="QIE41_004049"
     CDS             5685..5729
                     /gene="pheM"
                     /locus_tag="QIE41_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDS3532864.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5868..6851
                     /gene="pheS"
                     /locus_tag="QIE41_004050"
     CDS             5868..6851
                     /gene="pheS"
                     /locus_tag="QIE41_004050"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDS3532865.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGNWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6866..9253
                     /gene="pheT"
                     /locus_tag="QIE41_004051"
     CDS             6866..9253
                     /gene="pheT"
                     /locus_tag="QIE41_004051"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDS3532866.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWIGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9258..>9523
                     /gene="ihfA"
                     /locus_tag="QIE41_004052"
     CDS             9258..>9523
                     /gene="ihfA"
                     /locus_tag="QIE41_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDS3532867.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLK"
BASE COUNT         2522 a         2138 c         2453 g         2410 t
ORIGIN      
        1 ggctttggct gattacgttt atagcagcat aatatgtttc tacagtttta taatgattat
       61 gatgaatggc tatatgaaca gctgacatat tgtcatgatt tttagcggcc aacaatgtga
      121 ataactgaca ttgactaaaa gaatatttat ttgcaaaagt acccagcgtt gatatataag
      181 ataacacgac gtctttatta cccttgctca tggcgatgta taaagcaggg gttccatatg
      241 ctgttgcgcc ttttaataaa tccatgatgt taattttgct gaggttatat ttaacagcga
      301 ttccataaac tttagagagg aaccgtgtga cacacaaagg gtgattattt tccattgcgg
      361 catataatcc tggctcacca tcacgtgttt tcattgctaa aatatccatc accatttttt
      421 tgcttaaaag ctcttgtgtt attggagtgg ttaatgcatt cagaaagata tcgattattt
      481 catcgaatcc atacaacatg gagatataga gtccaggtgt ttcgttagca cttttagttt
      541 gcagtaattt aaccagatta tcttcatgaa tgatatgatt atctaccagt gactgaattt
      601 cttgcataaa tatttttacg ttgtcagtat gtccgttcat catagccaga aataaagcag
      661 gtgtaccaac gctattctgc gcagttatta tttcaatgat ttctttttca gggagtatgg
      721 gtaactgttt gaataatgca ataagctgtt gagtctggcc tgtggccatt attaacaaca
      781 ttgccgatgg atgtataggg gcaaatggca ttcgtgatgc aatacccgcg aaaatagccc
      841 ctggaactcc ctcaggtaat aggggcagaa aagcaattgc acagttgcga acatacatat
      901 cagcccaggt ctgagcatcg ggacctgaac tgatgaattt atcgagcgag agaaagcccc
      961 gtgctgtcct gtgggtgcct ctgacggcaa cgttggtatc gttcgggtcg taaacggaca
     1021 caacataatt taattgccca tacgctgact gctttattcg tagccttact gacaaagtat
     1081 gattgtctat atataaaatc gcggacatat gactgacatt gtttgccatc attcgacaga
     1141 agaaaatatc taaagcgaag ccaaaatcct tactcgcgat aacgcgactc cctgcaggag
     1201 gatggcgaag taatgatctg aactccgcgt cccggactaa gggaacatgc tgctgtatgg
     1261 attcaacgga ggagtaatgt ttaatcagat tgactttgcc ttttccattt ctcagtgaat
     1321 caagtgcgta ttgcaatgac agatggcgac aaaaaatagc gtcagaagga gattgcgaaa
     1381 aaaatgcact accattgaga tttactgttg gttgtatagt tttttctcta tggatttttt
     1441 tagacagttt catacaatgg ttacgatttt cggcagttaa ctgttcctgt tcaatataat
     1501 ccagtatgct tcgcaaatgg ttagctttaa taggcgtggt ttccagtaat aaggaaatga
     1561 tttctttttt atagtgtaat gtaatgagta tattttctgc atggatgggt gaagtggcaa
     1621 agttaatggt aaaaagggca gtggaataac aattttgtga tatttttcga cagtttgcgg
     1681 taaattcctg agtaacacag tatgacagca tcgaacttaa gtcagaggta tcaagtaggg
     1741 tgatgagttg gcgcgcaaat tgattaatag gctctgatga aggcgttggt atgtcgctat
     1801 cgatgtgcat tctatgagga gacttataat gagttctgat aataatgtcg ttttcactca
     1861 taatctattc gccttatata tgtctggagg ttttgaggat gatgattaat tattatcgcc
     1921 agaacgaaat gtgcacgaaa aattgctata agaattgtgt catttttatc attgtgatat
     1981 gaccacttcg tcattaatct tatgatcgtt taaatagtac ctccaaatca cgcaacgaac
     2041 tttgctggat tcagtttatg ctgctgcaaa tccgctcgca gtaaaccttt cagacgctcc
     2101 gtgatgttat cagtggttct ttaagcgttt tgctggtgta ctcactacaa acgaattgcg
     2161 aatcgaatca atgtgaaacg gaaaggtaca atctcccttt ttttagtgtt aacatcgctc
     2221 aaccgggtgg aaggtgagag gttaatgtct ttcatttgtg tacctgattg cgaaccaatt
     2281 tagcatttgt tggctagatg gtttcgcaat gaactgttaa taaacaaatt tttctttgta
     2341 tgtgatcttt cgtgtgggtc accactgcaa ataaggatat aaaatgcctg ttataactct
     2401 tcctgatggc agccaacgcc attacgatca cgctgtaagc cccatggatg ttgcgctgga
     2461 cattggtcca ggtctggcga aagcctgtat cgcagggcgc gttaatggcg aactggttga
     2521 tgcttgcgat ctgattgaaa acgacgcaca actgtcgatc attaccgcga aagacgaaga
     2581 aggtctggag atcattcgtc actcctgtgc gcacctgtta gggcacgcga ttaaacaact
     2641 ttggccgcat accaaaatgg caatcggccc ggttattgac aacggttttt attacgacgt
     2701 tgatcttgac cgcacgttaa cccaggaaga tgtcgaagca ctcgagaagc ggatgcatga
     2761 gcttgctgag aaaaactacg acgtcattaa gaagaaagtc agctggcacg aagcgcgtga
     2821 aactttcgcc aaccgtgggg agagctacaa agtctccatt cttgacgaaa acatcgccca
     2881 tgatgacaag ccaggtctgt acttccatga agaatatgtc gatatgtgcc gcggtccgca
     2941 cgtaccgaac atgcgtttct gccatcattt caaactaatg aaaacggcag gggcttactg
     3001 gcgtggcgac agcaacaaca aaatgttgca acgtatttac ggtacggcgt gggcagacaa
     3061 aaaagcgctt aacgcttacc tgcagcgcct ggaagaagcc gcgaaacgcg accaccgtaa
     3121 aatcggtaaa cagctcgacc tgtaccatat gcaggaagaa gcaccgggta tggtattctg
     3181 gcacaacgat ggctggacca tcttccgtga actggaagtg tttgttcgtt ctaaactgaa
     3241 agagtaccag tatcaggaag ttaaaggtcc gttcatgatg gaccgtgtcc tgtgggaaaa
     3301 aaccggtcac tgggacaact acaaagatgc aatgttcacc acgtcttctg agaaccgtga
     3361 atactgcatt aagccgatga actgcccggg tcacgtacaa attttcaacc aggggctgaa
     3421 gtcgtatcgc gatttgccgc tgcgtatggc cgagtttggt agctgccacc gtaacgagcc
     3481 gtcaggttcg ctgcatggcc tgatgcgtgt acgtggtttt acccaggatg acgcgcatat
     3541 cttctgtact gaagaacaaa ttcgcgatga agttaacgga tgtatccgtt tagtctatga
     3601 tatgtacagc acttttggct tcgagaagat cgtcgtcaaa ctctccactc gtcctgaaaa
     3661 acgtattggc agcgacgaaa tgtgggatcg tgctgaggcg gacctggcgg ttgcgctgga
     3721 agaaaacaac atcccgtttg aatatcaact gggtgaaggc gctttctacg gtccgaaaat
     3781 tgaatttacc ctgtatgact gcctcgatcg tgcatggcag tgcggtacag tacagctgga
     3841 cttctccttg ccgtctcgtc tgagcgcctc ctatgtgggc gaagacaacg agcgtaaggt
     3901 accggtaatg attcaccgcg caattctggg gtcgatggaa cgtttcatcg gtatcctgac
     3961 cgaagaattc gctggtttct tcccgacctg gcttgcgccg gttcaggttg ttatcatgaa
     4021 tattaccgat tcacagtctg aatacgttaa cgaattgacg caaaaactat caaatgcggg
     4081 cattcgtgtt aaagcagact tgagaaatga gaagattggc tttaaaatcc gcgagcacac
     4141 tttgcgtcgc gtcccgtata tgctggtctg tggtgataaa gaggtggaat caggcaaagt
     4201 tgccgttcgc acccgccgtg gtaaagacct gggaagcatg gacgtaaatg aagtgatcga
     4261 gaagctgcaa caagagattc gcagccgcag tcttaaacaa ttggaggaat aaggtattaa
     4321 aggcggaaaa cgagttcaaa cggcgcgccc taaccgtatc aatggcgaaa ttcgcgccca
     4381 ggaagttcgc ttaacaggtc tggaaggcga gcagcttggt attgtgagtc tgagagaagc
     4441 tctggagaaa gcagaagaag ccggagtaga cttagtcgag atcagcccta acgccgagcc
     4501 gccggtttgt cgtataatgg attacggcaa attcctctat gaaaagagca agtcttctaa
     4561 ggaacagaag aaaaagcaaa aagttatcca ggttaaggaa attaaattcc gtcctggtac
     4621 agatgaaggc gactatcagg taaaactccg cagcctgatt cgctttctcg aagagggtga
     4681 taaagccaaa atcacgctgc gtttccgcgg tcgtgagatg gcgcaccagc aaatcggtat
     4741 ggaagtgctt aatcgcgtga aagacgattt gcaagaactg gcagtggtcg aatccttccc
     4801 aacgaagatc gaaggccgcc agatgatcat ggtgctcgct cctaagaaga aacagtaagg
     4861 ccttcaagta gcactgtctg tggagccttt gggcttcaca ggcattgttc gcctttgttt
     4921 cgtttattaa caatgcgaag tggaagttat taaaatgcca aaaattaaga ccgtacgcgg
     4981 tgctgctaag cgcttcaaaa aaaccggtaa aggtggtttt aagcacaagc acgctaacct
     5041 gcgtcacatt ctgaccaaaa aagcgaccaa acgtaaacgt cacctgcgtc cgaaagccat
     5101 ggtttccaaa ggcgatctgg gcctggtaat cgcgtgcctg ccgtacgcat aagccgttaa
     5161 cgttttttaa ctttttaatt agaatataga tacaggagag cacatatggc tcgcgtaaaa
     5221 cgtggtgtta ttgcacgcgc acgtcacaag aaaattttga aacaagctaa aggctactac
     5281 ggtgcgcgtt ctcgcgtata ccgcgttgcc ttccaggctg ttatcaaagc tggtcagtat
     5341 gcttaccgtg accgtcgtca acgtaagcgt cagttccgtc aactgtggat tgcgcgtatc
     5401 aacgcagcag cacgtcagaa cggtatttct tacagcaaat tcatcaatgg cctgaaaaaa
     5461 gcctctgttg aaatcgaccg taagatcctg gctgatatcg cagtattcga caaagtagcg
     5521 ttcaccgctc tggttgaaaa agcgaaagca gctctggcat aagccagttg aaagagggag
     5581 ctagtctccc tcttttcgtt tcaacgccat caaaacattg acttttatcg ccgtagcctt
     5641 ttcaataaag gtcttttgaa aagtaccaaa aggtaacgca agcaatgaat gctgctattt
     5701 tccgcttctt tttttacttt agcacctgac tccaggaggc tagcgcgtga gaagagaaac
     5761 ggaaaacagc gcctgaaagc ctcccagtgg aggctttttt tgtatgcgcg ttttgaaaaa
     5821 attaagtcca acgaaccagt gtcaccactg acacaatgag gaaaaccatg tcacatctcg
     5881 cagaactggt tgccagtgcg aaggcggcca ttagccaggc gtcagatgtt gccgcgttag
     5941 acaatgtgcg cgtcgaatat ttgggtaaaa aagggcactt aacccttcag atgacgaccc
     6001 tgcgtgagct gccgccagaa gaacgcccgg cagccggtgc ggttatcaac gaagcgaaag
     6061 agcaggttca gcaggcgctg aatgcacgta aagcggaact ggaaagcgca gcgctgaatg
     6121 cgcgtctggc ggcggaaacg attgatgtct ccctgccggg gcgtcgtatt gaaaacggcg
     6181 gtctgcatcc ggtgacccgt accatcgacc gtatcgaaag tttcttcggt gagcttggct
     6241 ttaccgtggc aaccgggccg gaaatcgaag acgattatca taacttcgat gctctgaaca
     6301 ttcctggtca ccacccggcg cgcgctgacc acgacacttt ctggtttgac gctacccgcc
     6361 tgctgcgtac ccagacctct ggcgtacaga tccgcaccat gaaagctcag cagccaccga
     6421 ttcgtatcat cgcgcctggc cgcgtttatc gtaacgacta cgaccagact cacacgccga
     6481 tgttccatca gatggaaggt ctgattgttg ataccaacat cagctttacc aacctgaaag
     6541 gcacgctgca cgacttcctg cgtaacttct ttgaggaaga tttgcagatc cgcttccgtc
     6601 cttcctactt cccgtttacc gaaccttctg cagaagtgga cgtcatgggt aaaaacggta
     6661 attggctgga agtactgggc tgcgggatgg tgcatccgaa cgtgctgcgt aatgttggca
     6721 tcgacccgga agtttactct ggtttcgcct tcggaatggg gatggagcgt ctgaccatgt
     6781 tgcgttacgg cgtcaccgac ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac
     6841 agtttaaata aggcaggaat agattatgaa attcagtgaa ctgtggttac gcgaatgggt
     6901 gaacccggcg attgatagcg atgcgctggc aaatcaaatc actatggcgg gcctggaagt
     6961 tgacggtgta gaaccggttg ccggtagctt ccacggcgtg gtcgttggtg aagtggttga
     7021 gtgtgcgcag catccgaacg ctgacaaact gcgtgtgaca aaagtgaatg tcggcggcga
     7081 tcgcctgctg gacatcgtct gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt
     7141 agcgaccatt ggtgctgttc tgccgggcga tttcaaaatt aaagcggcga aactgcgtgg
     7201 cgaaccgtct gaagggatgc tgtgctcctt ttctgagctg ggaatttctg acgatcacag
     7261 cggcattatc gagctgcctg cagatgcgcc gattggcaca gacatccgcg aatacctgaa
     7321 actcgatgac aacaccatcg aaatcagcgt aacgccaaac cgggccgatt gcttaggcat
     7381 tatcggcgtg gcgcgtgatg ttgccgtgct gaaccagctg ccgctggttg aaccggaaat
     7441 cgttccggtt ggtgcgacca tcgacgacac gctgccgatt acagtcgaag cgccggaagc
     7501 ctgcccgcgc tatcttggcc gtgtggtaaa aggcattaac gttaaagcgc caactccgct
     7561 gtggatgaaa gaaaaactgc gtcgttgcgg gatccgatct atcgatgcag tcgttgacgt
     7621 caccaactat gtactgcttg aactgggcca gccgatgcac gctttcgata aagatcgcat
     7681 tgaaggcggc attgtggtgc ggatggcgaa agagggcgaa acgctggtgc tgcttgatgg
     7741 cactgaagcg aagctgaatg ctgacaccct tgtcatcgcc gaccacaaca aggcgctggc
     7801 gatgggcggc atctttggtg gcgaacactc tggcgtgaat gacgaaacgc aaaacgtcct
     7861 gctggaatgc gctttcttca gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct
     7921 gcataccgat gcgtctcacc gttatgagcg tggcgttgat ccggcactgc agcacaaagc
     7981 gatggaacgt gcgacccgtc tgctgatcga catctgcggc ggtgaggctg gcccggtaat
     8041 tgatatcacc aacgaagcaa cgctgccgaa gcgagccacc attactttac gtcgcagcaa
     8101 actggatcgc ctgatcggcc atcatattgc cgatgagcag gtaactgata ttctgcgtcg
     8161 tctcggctgc gaagtgaccg aaggcaaaga cgaatggcag gcagttgcgc cgagctggcg
     8221 tttcgacatg gagattgaag aagatctggt cgaagaagtc gcgcgtgttt acggctacaa
     8281 caacatccct gatgagccgg tgcaggcaag cctgattatg ggtactcacc gcgaagcaga
     8341 tctgtcgctc aagcgcgtga aaacgctgct caacgacaaa ggctatcagg aagtgatcac
     8401 ctacagcttc gttgatccga aagtgcagca gatgatccat ccaggcgttg aagccttact
     8461 gctgccaagc ccgatttccg ttgaaatgtc agcaatgcgt ctttccctgt ggatcggcct
     8521 gctggcaacc gtggtgtaca accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag
     8581 tggtctgcgt ttcgtaccag atactcaggc accgttgggc attcgtcagg atctgatgtt
     8641 agccggtgtg atttgcggta accgttacga agagcactgg aacctggcaa aagagaccgt
     8701 tgatttctat gatttgaaag gcgatctgga atccgttctc gacctgaccg gtaaactgaa
     8761 tgaggttgag ttccgtgcag aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat
     8821 ttatctgaaa ggtgaacgta ttggttttgt tggggttgtt catcctgaac tggaacgtaa
     8881 actggatctt aacggtcgca ctctggtgtt cgaactggag tggaacaagc tcgcagaccg
     8941 cgtggtgcct caggcgcgcg agatttctcg cttcccggcg aaccgtcgtg acatcgcggt
     9001 ggtggtcgca gaaaacgttc ccgcagcgga tattttatcc gaatgtaaga aagttggcgt
     9061 aaatcaggta gttggcgtaa acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg
     9121 gtataagagc ctcgccataa gcctgatcct gcaagatacc agccgtacac tcgaagaaga
     9181 ggagattgcc gctaccgtcg ccaaatgtgt agaggcatta aaagagcgat tccaggcatc
     9241 attgagggat tgaacctatg gcgcttacaa aagctgaaat gtcagaatat ctgtttgata
     9301 agcttgggct tagcaagcgg gatgccaaag aactggttga actgtttttc gaagagatcc
     9361 gtcgcgctct ggaaaacggc gaacaggtga aactctctgg ttttggtaac ttcgatctgc
     9421 gtgataagaa tcaacgcccg ggacgtaacc cgaaaacggg cgaggatatt cccattacag
     9481 cacggcgcgt ggtgaccttc agacccgggc agaagttaaa aag
//
LOCUS       DAOTRK010000166         9484 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.167, whole genome shotgun sequence.
ACCESSION   DAOTRK010000166 DAOTRK010000000
VERSION     DAOTRK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.167"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            98..808
                     /locus_tag="QIE41_004053"
     CDS             98..808
                     /locus_tag="QIE41_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDS3532868.1"
                     /translation="MQTRRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENEGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            850..3501
                     /locus_tag="QIE41_004054"
     CDS             850..3501
                     /locus_tag="QIE41_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDS3532869.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHLAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSIKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSATSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVIHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3515..4045
                     /locus_tag="QIE41_004055"
     CDS             3515..4045
                     /locus_tag="QIE41_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDS3532870.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKETTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLIPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4058..4561
                     /locus_tag="QIE41_004056"
     CDS             4058..4561
                     /locus_tag="QIE41_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDS3532871.1"
                     /translation="MKRLHKRFLLATFCALFTVTLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4621..5535
                     /locus_tag="QIE41_004057"
     CDS             4621..5535
                     /locus_tag="QIE41_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3532872.1"
                     /translation="MGKTISMKLLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            5868..8147
                     /gene="ydeP"
                     /locus_tag="QIE41_004058"
     CDS             5868..8147
                     /gene="ydeP"
                     /locus_tag="QIE41_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDS3532873.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            8395..8592
                     /gene="safA"
                     /locus_tag="QIE41_004059"
     CDS             8395..8592
                     /gene="safA"
                     /locus_tag="QIE41_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDS3532874.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            8667..9428
                     /gene="ydeO"
                     /locus_tag="QIE41_004060"
     CDS             8667..9428
                     /gene="ydeO"
                     /locus_tag="QIE41_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDS3532875.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNKGNTMNALAI"
BASE COUNT         2709 a         2064 c         2137 g         2574 t
ORIGIN      
        1 gaaacccggc gagtcgccaa tgtattagca acatttaccg attgtctgat gtaacggttt
       61 ccccgcttat cctcttaaca catggagaat gcatcagatg caaactaggc gtacccccta
      121 ctctatttcc tttatggcga cagtgttact tttattgctc tttgcttgcc atagcaccgt
      181 tgccaacgca gctgtcgcac tgggtgcgac gcgggtaatt tatccggcaa accagaaaca
      241 ggttttattg cctgtcacga ataatgatcc tgccagtgtc tatctcattc agtcatggat
      301 agaaaatgag ggtgaccaaa aagacaccca gtttgtcatt acgccaccgc ttttttccat
      361 gcaaggtaaa aaagagaata cgctgcggat aatcaacgcc actaatcatc agttaccagg
      421 cgaccgtgaa agcctgttct gggttaatgt taaagctatt cctgctatgg aaaaagatca
      481 gaagaatgag aatacattac agctggcaat tattagccgc ataaaaatgt tttatcgccc
      541 cacgaacctg gcaatggcac ctgaagaagc gcctgcaatg cttcggtttc gtcgcagcgg
      601 cagcaaactc actcttatta atccaacgcc ctacttcatt accgtaacaa atatgaaagc
      661 aggaaatagc aatctcccaa acaccatggt gccgccaaaa ggtgaagtat cggtggatat
      721 cccgcatgct gcaaccgggg atatcagttt tcagacgatc aatgattacg gtgcattaac
      781 tccgcgtatt aaggcaacca tgcaataagc cagtgaccta attaataata aactcaatcg
      841 gaattaaaaa tgacagcctt tcgtgccgca tttaaggcat atcgtatgca tcaggtgtta
      901 ctactgcctc gctttgcacg gcttaccatt gcccttggct tagcaactgc ggtatttccc
      961 gttgacgcag agtattattt taatccacgg tttttaagca atgatcttgc tgaatctgtc
     1021 gatttatccg cctttactaa gggacgcgag gcaccacctg gtacatatcg ggtcgacatt
     1081 tatttgaacg acgaatttat gactagtcgg gatattactt ttattgcaga tgataataac
     1141 gcagatctca tcccatgcct gagtacagat cttcttgtca gtcttggcat aaaaaaatca
     1201 gcgttattag acaataaaga acatttggca gaaaaacatg tgccggacaa cagcgcatgc
     1261 acaccacttc aggatcgttt ggccgacgca tcaactgagt ttgatgttgg tcagcaacat
     1321 ttgtcactct ccgttccgca aatttatgtt ggcagaatgg ctcgcggcta tgtttctcct
     1381 gatctgtggg aagaaggaat aaatgctggg cttctaaact atagttttaa tggtaattct
     1441 attaataatc gtagtaacca taatgcagga aaatccaact atgcatattt gaatttacag
     1501 agtggcatca acattggtag ttggcgactg cgcgataact caacgtggag ttataacagt
     1561 gggagtagca attcatctga cagcaataaa tggcagcaca tcaatacgtc ggctgaacgt
     1621 gacattattc ccttacgctc acgcttaacg gtaggtgata gttataccga tggcgatatt
     1681 tttgatagtg tgaactttcg tggcctcaaa ataaattcaa cagaagcgat gttgcccgat
     1741 agccaacatg gtttcgctcc ggtgattcat ggtattgccc gcggcaccgc acaagtgagt
     1801 gtaaaacaga atggatacga tgtttatcag actactgtcc cacccggccc ttttactatt
     1861 gatgatatca actctgcggc caatggtggt gatctgcaag taaccataaa agaggcagac
     1921 ggcagtattc agacattata tgttccttat tcgtctgttc cggttctcca acgtgctgga
     1981 tatacgcgtt atgcgcttgc catgggggaa tatcgtagtg gaaataacct gcaaagctcc
     2041 cccaagttca tacaaggtag cttgatgcat ggactggaag gaaactggac accttatggc
     2101 ggaatgcaaa ttgcagaaga ttatcaagcc ttcaaccttg gtattggtaa agatttagga
     2161 ctttttggtg ccttttcttt cgatatcacg caggccaata cgacacttgc agatggcacc
     2221 cgtcacagcg ggcaatcgat taaatccgtc tacagcaaat ccttctacca gacgggaacc
     2281 aatatccagg tcgcaggata tcgctattct acgcaaggtt tttataactt atccgacagt
     2341 gcctacagtc gaatgagtgg ttacaccgtc aagcctccta ccggagacag caatgagcag
     2401 acacaattta ttgattattt taatctgttc tacagtaagc gtggtcagga acaaataagc
     2461 atctctcagc agcttggaaa ttacggtacg acatttttca gtgccagtcg ccaaagttac
     2521 tggaacacgt cacgcagcga ccagcaaata tcatttggat taaatgtgcc gtttggtgat
     2581 attacgactt cgctgaatta cagctattcc aataatatat ggcaaaacga tcgggatcat
     2641 ttacttgctt ttacgcttaa tgttcccttc agtcattgga tgcgtacaga cagtcagtcg
     2701 gcatttcgta attcaaacgc cagttacagt atgtcaaacg atttgaaagg cggcatgacc
     2761 aatctatcgg gggtttatgg cactctgctg ccggataata acctgaatta tagcgttcag
     2821 gttggtaaca cccacggggg taatacatcg tctgccacca gtggttacag ttctcttaat
     2881 tatcgtggag cttacggtaa tactaatgtc ggttacagtc ggagtggtga cagcagccag
     2941 atttattacg gaatgagtgg tgggattatt gctcatgctg atggcatcac ctttggtcag
     3001 ccgctgggcg acacaatggt tctggttaag gctcctggcg ctgataatgt caaaatagag
     3061 aaccagaccg gaattcatac cgactggcgt ggctatgcca tattaccatt tgcgacagaa
     3121 tatagagaaa atcgtgtcgc tcttaacgcg aattcccttg cagataatgt tgaactggat
     3181 gaaaccgttg tcactgtcat cccaactcac ggtgctattg ccagagcaac atttaatgca
     3241 caaatcggcg ggaaagtatt aatgacgttg aagtacggta ataagagcgt tccattcggt
     3301 gcaattgtca tacatggtga gaataaaaat ggcagcattg tcgcggaaaa cggtcaggtt
     3361 tatctgactg gacttccaca gtcagggaaa ttacaggttt catggggcaa agataaaaac
     3421 tcaaactgta ttgtcgagta caagcttcct gaagtttctc ctggtacctt actgaaccag
     3481 cagacagcaa tctgtcgcta aggaacaata gattatgaaa tacaataaca ttattttcct
     3541 cggtttatgt ctggggttaa ccacctattc tgctttatcc gcagatagcg ttattaaaat
     3601 tagcgggcgc gtcctcgatt atggctgcac agtctcatcg gattcgctta attttaccgt
     3661 agatctccaa aaaaacagtg ccagacaatt tccaacgacc ggtagcacaa gtccagccgt
     3721 cccttttcag attacgttaa gtgaatgcag taaagagaca acgggggttc gggttgcatt
     3781 taacggtatt gaggatgcag aaaataatac tctgttgaaa ctggatgaag gaagcaatac
     3841 ggcctccggt ttgggtatag aaatactgga cggaaatatg cgtccggtga aattgaatga
     3901 ccttcatgcc gggatgcagt ggatcccact gataccagaa cagaacaata ttttgcctta
     3961 ctccgctcgt ctgaagtcaa ctcagaagtc cgtcaatccg ggactggtga gggcttcggc
     4021 aacctttacc cttgaatttc aataatcaga gtcagatatg aaaagacttc acaagaggtt
     4081 cctgttagct acgttttgcg cgttattcac agtaactctc caggccgccg atgtcactat
     4141 cactgttaat ggtcgggtag tcgctaaacc ctgcactatt caaaccaaag aagctaacgt
     4201 taatctcggg gatctttata cgcgcaatct gcaacaacct ggttctgcat caggctggca
     4261 caatattact ttgtcattaa ccgattgtcc ggttgaaaca agtgcagtga cggcaatcgt
     4321 gacaggttca actgacaata cgggttatta caaaaatgaa ggtactgccg aaaatattca
     4381 gatagagcta agggatgacc aggatgcgac gttaaaaaat ggcgatagca aaacggttat
     4441 tgttgatgag atcactcgta atgcacagtt tccacttaag gcaagagcta tcacggtgaa
     4501 tggaaacgca agccagggaa cgatcgaggc gctaatcaat gtgatctaca cctggcaata
     4561 acgtcgattc gattttctta gcctcctttt taaatcaatt ttttaacctt aggatatatc
     4621 atgggtaaaa caataagtat gaagcttctg ttcggtattt atcttctatt aatggcaggc
     4681 aaagtttttg ccttcagttg taatgtcgat ggcgggtcaa gtattggagc aggtacaacc
     4741 tctgtttatg ttaatctcga ccctgtaata caaccgggcc agaatctggt tgtagacttg
     4801 tctcagcaca taagttgctg gaatgattac ggcggctggt acgacactga tcatataaac
     4861 ctggtacaag gctcagcttt cgcaggctct ctacagtcat ataaaggatc gctgtactgg
     4921 aataatgtga cctacccctt cccgttgacg acaaatacta atgtattaga tattggtgat
     4981 aagacaccga tgccattacc gttaaaactc tatattaccc cagtcggtgc ggccggtgga
     5041 gttgtcataa aagccgggga agttattgcc cgtattcata tgtataaaat tgccacgtta
     5101 ggaagtggaa atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg
     5161 ccaacaggcg gctgcaccgt tgatagtcgt aatgtcaacg tgaatctccc ggactttcct
     5221 ggaagcgcag aaatacccct tggcgtatat tgctcaagtg aacaaaagct ctcattttat
     5281 ctttcgggtg ctaccactga cagtgccaga caagtgtttg ctaacacagc accagatgca
     5341 acaaaagcat cgggagtggg tgtctctctg atgcgtaatg gaaaaatact ggctacggga
     5401 gaaaacgtct ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc
     5461 tatggacaaa caggaaacaa ggttgcagcc ggaaccgtcc agtcagtcat tggtgtcact
     5521 tttatctatg aataattgtg attttgacat tggtctatgc gctccattca cgaaaattgc
     5581 gcggatattt attttgtgaa ggctattatc ctacatctgt aagaaaatcc gcgcacaatt
     5641 gaagccagag tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg
     5701 atgcttaatt tatcgtattt aatcttttgt ttaacgataa tagattaaaa tagaaagaga
     5761 gtagtcccct actcttatat atccatgttg acgataatcc ctttgtatat actgtgcatc
     5821 atcgctatta caaatcctaa taattcattt ccacacagga taagcagatg aagaaaaaaa
     5881 ttgaatccta ccagggagct gccggtggtt ggggtgctgt taaatccgta gcgaatgcag
     5941 tacgtaagca gatggatata cgccaggatg ttattgccat gtttgacatg aataagccag
     6001 agggctttga ctgtccgggt tgtgcatggc cagatcctaa gcacagtgcg tcattcgaca
     6061 tttgtgaaaa cggcgcaaaa gcaatcgcct gggaagtcac ggataagcag gtaaacgcct
     6121 ctttctttgc tgagaatacg gttcaatcat tacttacctg gggagaccac gagcttgagg
     6181 ctgcggggcg tctcacccag cctttgaaat atgatgccgt cagcgactgt tacaagccat
     6241 taagctggca acaagctttc gacgaaattg gcgaacgcct tcaaagctat agtgatccca
     6301 atcaggttga attctatact tcgggccgca cttccaatga agctgccttt ctttatcagc
     6361 tttttgcccg tgaatacggg agcaataact tccccgactg ctccaacatg tgccatgaac
     6421 cgaccagtgt gggtttggca gcgagtatcg gtgtaggtaa agggaccgtg ttgctggaag
     6481 actttgagaa atgcgattta gtcatttgca ttgggcataa ccctggtaca aaccaccctc
     6541 gcatgttgac ttcgttgcgc gctttagtga aacggggagc gaaaatgatc gccatcaatc
     6601 ctctacagga acgtggcctg gagcgattta ccgcaccgca aaacccgttt gaaatgctga
     6661 cgaactctga gactcagttg gccagtgcct actataacgt gcgcattggt ggtgatatgg
     6721 cgttgctcaa ggggatgatg cgcctgttaa ttgagcgcga tgatgctgca agcgccgcag
     6781 gtcggccctc attgctggat gacgaattta ttcaaacgca taccgtcggc tttgacgagc
     6841 tacgccgtga cgtgctcaat tccgagtgga aagatatcga acgtatttct ggattaagtc
     6901 agactcaaat cgccgaactg gctgatgcat atgccgctgc cgaacgcacc attatctgtt
     6961 acggaatggg gatcactcag cacgaacatg gtacccagaa tgtacagcaa ctggtcaatc
     7021 tgctgttgat gaaaggtaac attggcaagc ctggtgcggg tatctgccca ctacgaggtc
     7081 actctaatgt acagggcgac cgaaccgtcg gtatcaccga gaaaccgtct gcagagtttc
     7141 tggctcgcct gggtgagcgc tatggtttca ccccacctca tgcacctgga catgctgcaa
     7201 ttgccagcat gcaagcaata tgtacggggc aggctcgagc attgatctgc atggggggca
     7261 actttgctct ggcaatgcca gatcgggaag cgagcgctgt accgttaaca caattagatc
     7321 tgacggtaca cgtagccact aagcttaacc gctctcatct gctgaccgca cggcatagct
     7381 atattctgcc ggttctggga cgcagcgaga ttgacatgca aaaaagcggt gcgcaggcgg
     7441 taaccgttga ggattcaatg tcgatgattc atgcctcgcg tggcgtgtta aaacccgccg
     7501 gtgtaatgct gaaatcagag tgtgcagtgg tcgcgggaat cgcgcaggca gcactacccc
     7561 agagcgtggt agcctgggag tatctggtgg aagattatga tcgcattcgc aatgacattg
     7621 aagctgtgct gccagagttc gccgactata accagcgcat ccgtcatccc ggtggttttc
     7681 acctgataaa tgcagctgct gaaaggcgct ggatgacgcc gtcaggtaag gctaatttca
     7741 ttaccagtaa agggctgtta gaagatccct cttcagcgtt taacagtaag ctagtcatgg
     7801 cgacagtacg cagtcacgat cagtacaaca cgacgattta tggtatggat gatcgctatc
     7861 gaggggtatt cggtcaacga gatgtggtct ttatgagtgc taaacaagct aaaatttgcc
     7921 gtgtaaaaaa cggcgaaaga gttaatctta ttgcgcttac gccagacggt aagcgaagct
     7981 cacgccgcat ggatagatta aaagtggtca tttaccctat ggctgaccgc tcactggtga
     8041 cctattttcc agaatcgaat cacatgctaa cacttgataa ccacgatcca ttaagtggca
     8101 ttcctggcta taaaagtatt ccggttgaat tagaaccatc aaattaattc gcctcctcat
     8161 ttcttctgct gtcatccgca cagcagaaga attcctcatt gaccattgtc gcgcaattta
     8221 ctcacatgtg ctaaattaaa ctgctcgtta taagatatat acctccgcct acagctgtaa
     8281 gaaactccgc tcagtactga agcgccagtc ctatttcctc tattctccag cctgttatat
     8341 taagcatact gattaacgat ttttaacgtt atccgctaaa taaacatatt tgaaatgcat
     8401 gcgaccacag tgaaaaacaa aatcacgcaa agagacaact ataaagaaat catgtctgta
     8461 attgtggttg tcttattact gacacttacg ttgatagcca ttttttcggc aattgatcag
     8521 ctgggtattt cagaaatggg tcgcattgca agggatctta cacatttcat tatcaatagt
     8581 ttgcaagact gaaaataaac agcaaattat aaatatgaaa tgttaaaaaa gtatcgataa
     8641 aaactttatt gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc
     8701 atgccttcaa cgctaacatt ttagataaag attacgcctt ctctgacggc gagatcctga
     8761 tggtagataa cgctgttcgt acgcattttg aaccttatga gcggcatttc aaagaaatcg
     8821 gatttaatga aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg
     8881 tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa
     8941 tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt
     9001 tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg
     9061 tgctatccgt ttctgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga
     9121 agctgaaaga tatttgtgac tgcctgtaca ttagtgaaag cctgttgaag aaaaaactaa
     9181 agcaagagca aacgacattc tcacagattc ttttagatgc cagaatgcag cacgcaaaaa
     9241 atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgcgagta
     9301 catcttattt tatttatgcg ttccgcaaac attttggcaa cagtccaaag agagtttcta
     9361 aggagtaccg ttgtcaaagg cacacgggta tgaataaggg caacacgatg aatgctttag
     9421 ctatttgatt atttgctaac gagtagtcaa ccacgcaccc tacgtaaaaa ttaaaatggg
     9481 gcag
//
LOCUS       DAOTRK010000167         9374 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.168, whole genome shotgun sequence.
ACCESSION   DAOTRK010000167 DAOTRK010000000
VERSION     DAOTRK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.168"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1745
                     /gene="adeD"
                     /locus_tag="QIE41_004061"
     CDS             <1..1745
                     /gene="adeD"
                     /locus_tag="QIE41_004061"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDS3532876.1"
                     /translation="FHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPI
                     VIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPR
                     GLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTL
                     EQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAY
                     IAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTD
                     DRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQAD
                     IVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFAL
                     QFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLG
                     GFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNCQVQSHLPL
                     PIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLF
                     DGEKFAFTTLEVTE"
     gene            complement(1792..3183)
                     /gene="uhpT"
                     /locus_tag="QIE41_004062"
     CDS             complement(1792..3183)
                     /gene="uhpT"
                     /locus_tag="QIE41_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDS3532877.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3439..4758)
                     /gene="uhpC"
                     /locus_tag="QIE41_004063"
     CDS             complement(3439..4758)
                     /gene="uhpC"
                     /locus_tag="QIE41_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDS3532878.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4768..6270)
                     /gene="uhpB"
                     /locus_tag="QIE41_004064"
     CDS             complement(4768..6270)
                     /gene="uhpB"
                     /locus_tag="QIE41_004064"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDS3532879.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNIWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHSVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6270..6860)
                     /gene="uhpA"
                     /locus_tag="QIE41_004065"
     CDS             complement(6270..6860)
                     /gene="uhpA"
                     /locus_tag="QIE41_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDS3532880.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7022..8491)
                     /locus_tag="QIE41_004066"
     CDS             complement(7022..8491)
                     /locus_tag="QIE41_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532881.1"
                     /translation="MVSVTSYTYPTETENDNSQFQSQTQSQGLSQSLRDSIEGNIAAF
                     DAMEQSGLLDNCRTLSKNASFREIFRSLLLYILDSLHIYRHDISDKLVSYFHVTPESA
                     NANYKTWIATTGMDSYIKIEQTGSDKFKVTDVYRELNGKSLEKKSRDRDFSGRNVKEF
                     IGLLSEHDVLEKYQLSKVTVAANALKLEVVNNNFKNELSSGVTDAEHQLKKALYEQTQ
                     STPDKSIQYELIPDKLIPDDCSYKSDIKKLDVREGEFSALLADASRHVGEASSFIACC
                     GVKINSTIHELLRSKTMALSGHGGVRKYKDFEKLFSEIVFTPDECNIVRNSLQKDGVE
                     KKTRENIISCLSSISGIGNLYYACSQAFPVALDRGDINAIQASNSISAGFNSNPGFCA
                     LMGKTNFLFADFSLHTFTQTHYIVKAEINEAGNDFNIANFIYRTYGEDYIHNDSVDDS
                     LNERSTEDYRFLDDSLLPENDFRNRRRNFSGNIAIATYK"
     gene            complement(8767..9249)
                     /locus_tag="QIE41_004067"
     CDS             complement(8767..9249)
                     /locus_tag="QIE41_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532882.1"
                     /translation="MVELVYFNDNDCARARELLKKSNGNIADDKIDEIIACLSSHQGI
                     TCFYYSSVQGYQSYLKNTSQDVSELLSKSLDGAISSISGLKNSGNTFFGTGKYIKEID
                     FTINGFSQKMYSMAVNNVGNEVKLQCSRYFTTGRVDEQNSKIDKVTFTFFGAATTADL
                     "
BASE COUNT         2516 a         2500 c         2148 g         2210 t
ORIGIN      
        1 aatttcatca cattagccgg gcggaatacc aggaattgtt agccgtttcc cgtggcgacg
       61 ctgttgccga ttatattatt gataatgtct ctattctcga cctgatcaat ggcggagaaa
      121 tttccggccc aattgtgatt aaaggacgtt acattgctgg tgttggcgca gaatacactg
      181 atgctccggc tttgcagcgg attgatgccc gcggcgcaac ggcggtgcca gggtttattg
      241 atgctcacct gcatattgaa tccagcatga tgacgccggt cacctttgaa accgctaccc
      301 tgccgcgcgg cctgacaact gttatttgcg accctcatga aatcgtcaac gtgatgggcg
      361 aagccggatt cgcctggttt gcccgctgtg ccgaacaggc aaggcaaaac cagtacttac
      421 aggtcagctc ttgcgtaccc gccctggaag gctgcgatgt taacggtgcc agttttaccc
      481 ttgaacagat gctcgcctgg cgggaccatc cgcaggttac cggccttgca gaaatgatgg
      541 actaccctgg cgtaattagc gggcagaatg cgctgctcga taaactggat gcatttcgcc
      601 acctgacgct ggacggtcac tgcccgggtt tgggtggtaa agaacttaac gcctatattg
      661 ctgcgggtat tgaaaactgc cacgaaagtt atcagctgga agaaggacgc cggaaattac
      721 aactcggcat gtcgttgatg atccgcgaag ggtccgctgc ccgcaatctc aacgcactgg
      781 caccgttgat caacgaattt aacagcccgc aatgcatgct ctgtactgat gaccgtaacc
      841 cgtgggagat cgcccatgaa ggacacatcg atgccttaat tcgccgcctg atcgaacaac
      901 acaatgtgcc gctgcatgtg gcatatcgcg tcgccagctg gtcgacggcg cgccactttg
      961 gtctgaatca cctgggctta ctggcacccg gcaagcaggc cgatatcgtc ctgttgagcg
     1021 atgcgcgtaa ggtcacggtg cagcaggtac tggtgaaagg cgaaccgatt gatgcgcaaa
     1081 ccttacaagc ggaagagtcg gcgagactgg cacaatccgc tccgccatat ggcaatacca
     1141 ttgcccgcca gccagtttcc gccagcgact ttgccctgca attcacgccc ggaaaacgct
     1201 atcgggtaat tgacgtcatc cataacgaat tgattaccca ctcccgctcc agcgtctaca
     1261 gcgaaaatgg ttttgatcgc gatgatgtgt gctttattgc cgtacttgag cgttacgggc
     1321 aacggctggc tccggcctgt ggtttgctcg gcggctttgg cctgaatgaa ggtgcgctgg
     1381 ctgcgacggt cagccatgac agccataata ttgtggtgat cggtcgcagt gccgaagaga
     1441 tggcgctggc ggtcaatcag gtgattcagg atggcggcgg gttgtgcgtg gtacgtaact
     1501 gccaggtcca aagccatctg ccgttaccca ttgccgggct gatgagcacc gacacggcgc
     1561 agtcgctggc ggaacaaatt gacgccttga aagctgccgc ccgtgaatgc ggtccgttac
     1621 ccgatgagcc gtttattcag atggcgtttc tttctctgcc agtgatcccc gcgctgaaac
     1681 taaccagtca ggggctattt gatggcgaga agtttgcctt caccacgctg gaagtcactg
     1741 aataataaaa aaaagcccgg cgtcatgccg ggcaaaagtc accagttacg tttatgccac
     1801 tgtcaactgc tgaatttttt tctcgcggcg gattttgcgt tcttccatca ccgccactat
     1861 cgccatcagg cagatacaac caatcgcggc gatatcaagt gcggcgaagg tgcctgccca
     1921 gccggtaagg ccgaataccg gcgtcccatc ggcaatcatt cccagaccta acttggcaaa
     1981 gctgtcacca atcaggtaag cgaaggtgcc tttaatacca tcggcagcgc caatcgcttt
     2041 tttaggtaca aagccaacag cagccacacc aatcaacaat tgcgggccaa agaccaggaa
     2101 acccaacgca aagagagaag ccaggtagat atattcgttg ctggcatgtt gatacacacc
     2161 gagcgtggcg ataatcagcg ccagcgcgat gcaggccacc aggccacggc gaccgttcgc
     2221 caggtcagag agccagcccc acagcagcgt accgaccagc gcaccagctt caaacagcgt
     2281 aaagccctga atcgccaccg ctttagagag tttcagttcc tggaacgcat acacggttga
     2341 ccactggtcg ataccaatac gcaccacata gaggaaaatg ttggcgaagc acagcagcca
     2401 gatcactttg tttttcagca catactcaac aaagatctgc cacttggtca tatcggtaga
     2461 ttctgtctct ttatcctctt cactgatctc ctcgccaaac agctcttcag ctttgccgag
     2521 gccataagat tccggggagt cgctgccgta acgcaggccg ataaaaccaa caatcagcgc
     2581 gataatcgac gggaagataa acatgccgat aacatgacca tcgaacaggt aatttgcgcc
     2641 gaacagcgcc acacctgctg cgcctgcacc gccaaggttg tgagaaatgt tccagaaacc
     2701 gaggaacgtc ccgcgtttac gacgcggcgt ccatttggtg atggtggagt aactgcacga
     2761 acccccggta ctctggaaaa agccacttaa ggcgtagaag gcaatcatca ggaacaggct
     2821 aaccgagccg ctgcccatac tggcgctaaa gcccagcata caaatggcag acaggatcag
     2881 catgaacggc aggaattgtt tggtgttttt gccgtcggcg tagtaggaaa ccagcgtttt
     2941 acccacgcca taagtgatgg agaaacccag gccgatcatc cccagctgcg tcatgctcaa
     3001 cccgtaggtc gaaatcatat cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat
     3061 cgtcaggtag ccgataaaga ccaccaggta ggattgcatg aacggtttga accacatttt
     3121 gcgccgcact tcgagcggaa ggtccagggt cggcttgcgg acctggttta agaaagccag
     3181 catgggttac tcctgatttc ccttcatact tcaagccgca ggtgccttag ctgcacgcat
     3241 tcctcccggt cacatagttc actatgctcc cgggactcac gcgtttgctg cgttcctgca
     3301 acttgaatta ttttgggaaa gaattaatga atgatggcat tgtaaaaatc agcagcaaat
     3361 caggcctgag atagcgtcca ggcaaaacct ggaaaatttc ttagttttgc cgggtatgaa
     3421 gtgaaaaggt gagatgcatc acgcttctcg cggtgtctgg gcgttcaaaa agggcagtaa
     3481 cagcagtgcg gaaatcccgg cggcgataga gataaccaca aaaaatccgc tccagtgcca
     3541 ggtatcgagt actttcgcca gtggccaacc agcaagcgac gcccccagat aagcaaacaa
     3601 gccgacaaac cccgtcgccg cccctgccgc ctctttgtgg gaacattctg ccgccgccat
     3661 gccgattaac atctgcgggc caaaaacaaa aaaaccaatg gtgaagaagc aggttgcctg
     3721 catcacgtag ctggcaaatg gcatcagcca cagcgagccg actgaaagca aaattccggc
     3781 ggcgaaaatc aaattcatcg gcccgcggtt gccgttaaac aatttgtccg agccccaacc
     3841 cgctaccagc gcaccgataa atccgcccag ttcaaacatt gtcactgccg tattcgccgt
     3901 gaccagatcg acgcccagcg tctcggacat atacaaattg ccccagtcgt tgattgccgc
     3961 ccggaccaca tagaccagca catagcaaaa cgaaagcagc cagatatacg gattcaacaa
     4021 cacatacttg gtgaggatct ctttacgcgt cagccctgcc ccttcttgtt gttgagcaat
     4081 ctccagcgcg tcgtgtcgcc attcaccaac cgcaggtaaa cctaacgcct gcgggcgatc
     4141 gcgcagccgc cagcagagaa aaatccccac gacgatcgcc atacaaccag caatcatcat
     4201 cccggcacgc cagccgtaat gcagcgcagc agctgccatc acaatgggaa tgagcgcacc
     4261 accaacgtta tgcgccgtgt tccataatgc ccaccaacca ccgcgctcgg tacgagaata
     4321 ccaggccgtt aacagacgcg cacacaccgg tgaaccccag ccctggaaaa aggcgttcag
     4381 cacccagagc acggcaaacg cccatagcga cgtcgagaag ccaaacagaa tgttgatgat
     4441 gcccgtggcg ataagcccta tccccataaa ataacgggca tttgagcgat cgctgacaat
     4501 gccggagaca aacttcgaca cgccgtaggt aatgtaaaac agggtcgcta acaggccgat
     4561 atcgctacgg ctgagcacgc cattagcaag gatttctggt acggcggcgt taaaactttt
     4621 ccgcgtgaag taaaacagcg cgtaacccag ccagatggtc agcagaatat gccgacgcca
     4681 gtagcgataa cgggcatcaa tttcatgttt atcagtcatt aatggcgcat cggcaggcgc
     4741 tttcagaaac ggcaacatca tcaaacctta gacatagcgt tgaggtagag aaacgctgac
     4801 acgcgtgccg tgcagacagg aaatggtcaa ggtgccgccc agcgccgtta cgcgctcgcg
     4861 cattccggtg aggccaaaac cttgttgccc ggaacccggc ggcaaaccgc tgccatcgtc
     4921 ttcaatcacc agcatcaacc gttcatcctg ctgccagcct tgcagggtga ccgcgctggc
     4981 atcggcatgt ttcacaatgt tgttcagccc ttcctggcag acgcgaaaca gcgtcactcg
     5041 ctggttttca cttaacgctg attcatcgat tcgccattcg agatggctga caatgccgcg
     5101 cccttccagc tccatttccc gcatcagtga gcggatggcc tgctccaggg tgagatcatc
     5161 caactggcgc ggacgtaacc gacctaacaa acggcgcacc gcatcgtaaa cgcccagcga
     5221 cagttgttcg atgagctgcc cgctctgctt cacactggcg ttatctgccg caagtcgctg
     5281 gacaatgccc gcttgagtac gaatagcagt gatggtctga ccgatatcat cgtgcagctc
     5341 acgcgccacg tcgcggcgca cgctctcttc agtttctaac aaacgttcag ccagatgctg
     5401 attgcgcgcc agttcctttt gcagcgactg gttaagttca cgcaaccgct ggatgcctgc
     5461 gcccagtaac aaccctgtca gactttgcac cagcagcgag agcaataaat ccaccgaatg
     5521 atcgcgccag gtttgactgg cgatcagcgc gatggcgttc atcaacgtcg caatcagcgc
     5581 cccttgccag ccatagtgcc aggccagcgc gataatcggc agcgccaggc agaatggcgt
     5641 aaagcgcgac agttcgtccg gcaatcccaa ctggagccag agactaataa caaacagcag
     5701 caagtaccag accagatgcc gcccgcgcca gttgatcggt tgagaaacta acgacggccc
     5761 gagcggcagc cagatgttat tggcgagata gtgccagaac accagacata tcggggccag
     5821 cgtcaagccg ccagttaaag tcagcaacag cgcattccac gactctttgc cgtgccaaag
     5881 ccagggcagc gactgcaaca acgccgccgc cgtcagcgcc gcaccctgta acagcaaggt
     5941 gcgccagtca cgttgatggc gatagcgcga gatcagcgcc acgggcaata acgtcagtaa
     6001 actaccgatc atcaataagg agaaatgggt taaaccgacc gcctgcgtta gccagtaaat
     6061 cagcagccat tccgcgccca gcaatacggg ccagtatccg cgcgggcatt gcagcattag
     6121 ccccagacgc agaccaaacg gaaataacag caccgccata tcagggcgct caaccaggtg
     6181 caggctgata ctccacaggc aaaaccatgc ggcagagaag ataaaaaagc aggcaataac
     6241 ggtaattaag cgggagaaca acgtcttcat caccagccat caaacatgcg gcgcgccagt
     6301 tcaacgtcgt tactgacgcc cagtttttcc atcagattgg cgcgatggac gtgtaccgtt
     6361 ttcggtgaca agcccagttc ggcggcaatc tctttcaccg ccattccttg cgccagtttt
     6421 tccgccacct ggcgttcacg tttggttagc gggtcctggc gaccggatgc cagtttaatg
     6481 gcaatatccg gcgtcagata acagccgccc gtggcaaccg tatgcaccgc agcaatcagt
     6541 tcatccgggc tacagcgttt ggagagaaag ccgcgcgccc ccgcgttaag cgcctgctca
     6601 accagcgccg gactgtcatg aacagagagc ataatcgtcg ccataccttt cggcagctgg
     6661 cttagcagct ccagaccgga gatatcaggc atggagatat cgcaaataca cacctgcaca
     6721 ccgcgccccg gcaaccccgc cagcgcctcg cgccccgaac caaactcggc aactacttgc
     6781 aaatcaggtt ccagccccag caactgcgca aagccggagc ggacgatgag gtgatcgtct
     6841 ataagggcaa cggtgatcat aattgggtcc tggcggataa aaaacgcgca taccttaacg
     6901 ataagcgcga tgttgttcaa gttggatatg gggtatgtat tggcttaatg atgttgggct
     6961 tatcgtcaaa acgcttacct gaaaggtaag cgtttggtac gtattattga gaaaaagcga
     7021 attatttgta ggttgcgatt gcaatattgc ctgaaaaatt tctcctcctg ttgcggaaat
     7081 cgttttcagg taacaaagag tcatccagaa acctataatc ttcagtggaa cgctcattaa
     7141 gagagtcatc cactgagtcg ttatgaatat aatcctcacc gtatgttcga taaatgaaat
     7201 tagcaatatt aaaatcgtta ccagcttcat ttatttcggc ttttacaata taatgagttt
     7261 gtgtaaatgt atgcagtgaa aagtcagcaa aaagaaaatt tgtttttccc atcaaagcac
     7321 aaaaccctgg gttactatta aacccggcag aaatagaatt acttgcctgt attgcattaa
     7381 tatcccccct atcaagcgcg acaggaaatg cctgtgaaca agcataatac aggttaccta
     7441 ttcctgaaat actggagagg caagaaataa tattttctct ggtttttttc tcaacaccat
     7501 ctttctgtag agagtttctc acaatattac attcatctgg cgtaaataca atttcactaa
     7561 aaagtttttc gaaatcttta tattttctaa cacctccatg accagacaaa gccatggttt
     7621 tactcctcag taattcatgg atagtgctat ttattttaac tccacaacat gcaataaatg
     7681 agctagcctc tccaacatgt cttgatgcat cagcaagaag tgcactaaac tctccctccc
     7741 gaacatctag tttttttatg tctgatttat aactacaatc atctggaatt aatttatctg
     7801 gaatcaattc atattggatt gatttatctg gagttgattg agtttgttca tataacgctt
     7861 tttttaattg gtgctcagca tcagtaactc cactgctgag ttcattttta aaattattat
     7921 taacaacttc taacttaaga gcatttgcag caactgttac ttttgagagt tgatactttt
     7981 ccaatacatc gtgctctgat aataacccta taaactcttt cacatttcga ccactaaaat
     8041 ccctatccct actctttttt tccaaactct tgccatttaa ctcacgatac acatcagtga
     8101 ctttaaattt atcactgccg gtctgctcaa tttttatata gctatccatg cctgtggtag
     8161 cgatccatgt tttataattc gcattcgctg attcaggcgt aacatgaaaa taagaaacca
     8221 atttatcact aatatcatgt cgatagatat gtaaggaatc taagatgtat aacaataaac
     8281 ttcggaaaat ttcacgaaaa gaggcatttt tagacaatgt tctacaatta tcgagcaggc
     8341 cgctttgctc catcgcatca aatgcagcaa tattaccctc gatactatct cttagactct
     8401 ggctcaggcc ctgactctga gtttgactct gaaactggct attatcattc tctgtttccg
     8461 ttggatatgt ataactcgtc acactaacca tagtaacctc gcaatatgac actttgcata
     8521 ttttgacatt ttagaagcat caatggttag catgataaat atgtcaatgc taaccgctat
     8581 atttcattta tttcaataat aagaccatgt gtcgttaatt tttaatgatt ttcccaatgc
     8641 aattgcatca ctaattctac ttattcacta tcaaaaatcg ctcattattt caaaaagact
     8701 gtttatacac agacggttaa caatacgcgt tcaccttaat gataaacgcg atttatcagg
     8761 caaaaatcat aaatcagcag ttgttgcggc accgaaaaat gtaaaagtga ctttatcgat
     8821 tttggagttt tgttcgtcga ccctaccggt agtaaaataa cgagaacact gaagcttaac
     8881 ttcatttcca acattattaa ccgccatcga atacatcttc tgactaaacc catttatagt
     8941 aaaatcaatt tctttaatat atttcccagt cccaaaaaag gtatttcctg agttttttaa
     9001 acccgatatt gatgaaatgg caccatccaa actctttgag agtaactcgc taacatcttg
     9061 agatgtattt tttaaatagc tctgatagcc ttgtacggaa gaataataaa aacatgtaat
     9121 cccctgatga ctggataagc aagcgattat ttcatcaatc ttatcatccg caatgttacc
     9181 atttgatttc tttaataatt cccttgcccg agcacagtca ttatcattaa agtaaaccag
     9241 ttcaaccatt tgttcaagtg tgttttgact attctttgta tcttgttcaa ttatttcaat
     9301 tttctctgca aatattttaa gaatatcttt acttaactca acaccgcagc aagttatttt
     9361 agtagcacca tttt
//
LOCUS       DAOTRK010000168         9238 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.169, whole genome shotgun sequence.
ACCESSION   DAOTRK010000168 DAOTRK010000000
VERSION     DAOTRK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.169"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            126..1100
                     /gene="cysB"
                     /locus_tag="QIE41_004068"
     CDS             126..1100
                     /gene="cysB"
                     /locus_tag="QIE41_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDS3532883.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1431..1559
                     /locus_tag="QIE41_004069"
     CDS             1431..1559
                     /locus_tag="QIE41_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDS3532884.1"
                     /translation="MPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKVWG"
     gene            1562..1729
                     /locus_tag="QIE41_004070"
     CDS             1562..1729
                     /locus_tag="QIE41_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532885.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1843..1938
                     /locus_tag="QIE41_004071"
     CDS             1843..1938
                     /locus_tag="QIE41_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532886.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2102..4777
                     /gene="acnA"
                     /locus_tag="QIE41_004072"
     CDS             2102..4777
                     /gene="acnA"
                     /locus_tag="QIE41_004072"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDS3532887.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIYALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGIAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSEVISIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLIGEEKIDIVDLQSLQPGATVPVTFTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4841..5431)
                     /gene="ribA"
                     /locus_tag="QIE41_004073"
     CDS             complement(4841..5431)
                     /gene="ribA"
                     /locus_tag="QIE41_004073"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDS3532888.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5601..6365
                     /gene="pgpB"
                     /locus_tag="QIE41_004074"
     CDS             5601..6365
                     /gene="pgpB"
                     /locus_tag="QIE41_004074"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDS3532889.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKIHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPVEENREIAQREQES"
     gene            6514..6822
                     /gene="lapA"
                     /locus_tag="QIE41_004075"
     CDS             6514..6822
                     /gene="lapA"
                     /locus_tag="QIE41_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDS3532890.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            6829..7998
                     /gene="lapB"
                     /locus_tag="QIE41_004076"
     CDS             6829..7998
                     /gene="lapB"
                     /locus_tag="QIE41_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDS3532891.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYALYWHCPSCRAWSTIKPIRGLDGL"
     gene            8190..8927
                     /gene="pyrF"
                     /locus_tag="QIE41_004077"
     CDS             8190..8927
                     /gene="pyrF"
                     /locus_tag="QIE41_004077"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDS3532892.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQASAAGVDYM
                     VIGRPITQSVDPAQTLKAINASLQRSA"
     gene            8927..>9238
                     /gene="yciH"
                     /locus_tag="QIE41_004078"
     CDS             8927..>9238
                     /gene="yciH"
                     /locus_tag="QIE41_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDS3532893.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VK"
BASE COUNT         2281 a         2159 c         2434 g         2364 t
ORIGIN      
        1 acactaaggc tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt
       61 attagtcacc cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt
      121 ttaacatgaa attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg
      181 tctcatcaac ggcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa
      241 tgctggaaga cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg
      301 taacgccagc aggacaagaa ataattcgta tcgctcgtga agtcctgtcg aaagttgatg
      361 ccataaaatc ggtcgccgga gagcacacct ggccggataa aggctcgctg tatatcgcca
      421 ccacgcatac ccaggcacgc tacgcgttac caaacgtcat caaaggcttt attgagcgtt
      481 atccccgcgt ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgctgtct
      541 ctaaaggcaa tgccgatttc gctatcgcca cagaagcgtt gcatctgtat gaagatctag
      601 tgatgttacc gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg
      661 caggcaaaaa agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct
      721 tcggctttac cggacgttca gagctggata ctgcctttaa ccgcgcaggg ttaacgccgc
      781 gtatcgtctt cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg
      841 gggtaggggt cattgccagc atggcggtgg atccggtcgc cgatcccgac ctggtgcgcg
      901 ttgatgctca cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt
      961 ttttgcgcag ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg
     1021 tcgttgatgc ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata
     1081 taaaactgcc ggaaaaataa tttcttgcgt tattttcggc acctttcatg tagcgaaggt
     1141 gccggaatat attctctttt gttgcttatt tgtataccgt gcacaatatt tttatccctc
     1201 atatttatag ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaactccag
     1261 ttttcaaata tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat
     1321 tcgctggatt tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc
     1381 aatcgttcaa cgtctttctg ataagtgatg gcgattatat gaggttagca atgccttctg
     1441 gaaatcagga accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc
     1501 ttggttctgc acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac
     1561 tatggtcggt caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac
     1621 agaaacgaaa cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca
     1681 gcaagccttt atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt
     1741 cttattaccc atttttaatg aattacaggg cttttaatac accgcagcaa taacaggttg
     1801 agttatctca acacaaagta ataaccgtta aaggtgtagc ctatgatcaa cacaaatatg
     1861 aaatattggt cctggatggg cgcattttct ctgtcgatgc tcttctgggc cgaactcctc
     1921 tggatcatta ctcactgatc cctgaccccg ctgcggcggg gttttcattt gttttgccac
     1981 aagctttctc ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt
     2041 atctttaata ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc
     2101 tatgtcgtca accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta
     2161 ccactactac agcctgccgc ttgctgccaa atcactgggc gatatcaccc gtttgcctaa
     2221 gtcactcaaa gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga
     2281 agaggatatc tacgcgctgg cgggatggct gaaaaatgcc catgctgacc gtgaaattgc
     2341 ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc gtacctgccg ttgttgatct
     2401 ggcggcaatg cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct
     2461 ctcaccggtc gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga
     2521 ggcatttgaa gaaaacgtgc gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct
     2581 gaaatgggga aagcaggcgt tcagtcgttt tagcgtcgtg ccgccaggca caggcatttg
     2641 ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg agcgaattgc aggacggtga
     2701 atggattgct tatccggata cactcgttgg tactgactcg cacaccacca tgatcaacgg
     2761 ccttggcgtg ctggggtggg gcgtgggggg gatcgaagca gaagccgcaa tgttaggtca
     2821 gccggtttcc atgcttatcc cggacgtagt gggctttaaa ctcaccggaa aattacgtga
     2881 aggtattacc gccacagacc tggttctcac cgttacccaa atgctgcgca aacatggcgt
     2941 ggtggggaaa ttcgtcgaat tttatggtga tggtctggat tcactaccgt tggcggatcg
     3001 cgccaccatt gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga
     3061 tgctgtcacc ctcgattaca tgcgtttaag cgggcgcagt gaagatcagg tcgagttggt
     3121 cgaaaaatac gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac
     3181 cagtacgtta gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgccc
     3241 acaggatcgc gttgcactgc ccgatgtacc aaaagcattt gccgccagta acgaactgga
     3301 agtaaatgcc acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta
     3361 tcagttacct gatggcgctg tggtcattgc agcgataacg tcgtgcacca atacctctaa
     3421 cccaagtgtg ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc tgggcctcaa
     3481 gcggcaaccg tgggtcaaag cgtcgctggc accaggctcg aaagttgttt ctgactatct
     3541 ggcaaaagcg aaattgacac cgtatctcga cgaactgggg tttaaccttg tgggatatgg
     3601 ttgtaccacc tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa
     3661 aaaaggcgat ttaaccgtcg gtgcagtgct gtccggcaac cgtaactttg aaggccgtat
     3721 ccatccgctg gttaaaacca actggctggc ctcgccgccg ctggtggttg cctatgcgct
     3781 ggcgggaaat atgaatatca acctcgcatc tgagcctatt ggtcacgatc gcaaaggcga
     3841 tccggtatat ctgaaagata tctggccatc ggcacaagaa attgcccgtg cggtagaaca
     3901 agtctccaca gaaatgttcc gtaaagagta cgcagaagtt tttgaaggca tagcagagtg
     3961 gaaggaaatt aacgtcgcac gatccgatac ctacggttgg caggaggatt caacctatat
     4021 tcgcttatcg cctttctttg atgaaatgca ggcaacacca gcacctgtgg aagatattca
     4081 cggtgcgcgg atcctcgcaa tgcttgggga ttcagtcacc actgaccata tctctccggc
     4141 gggcagtatt aagcccgaca gcccagcggg tcgatatcta caaggccggg gtgttgagcg
     4201 aaaagacttt aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac
     4261 cttcgccaat attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg
     4321 gcatttacct gacagcgagg tcatctctat ttatgatgcc gcgatgcgct ataagcagga
     4381 gcaaacgccg ctggcggtga ttgccgggaa agagtatgga tcaggctcca gccgtgactg
     4441 ggcggcaaaa ggtcctcgtc tgcttggaat tcgtgtggtg attgccgaat cgtttgaacg
     4501 aattcaccgt tcgaatttaa ttggcatggg catcctgccg ctagaatttc cgcaaggcgt
     4561 gacgcgtaaa acgttagggc taatcgggga agagaagatt gatattgtcg atctgcaaag
     4621 tctacaaccc ggcgcgacgg ttccggtgac gtttacgcgc gcggatggta gccaggaagt
     4681 cgtaccctgc cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg
     4741 cattttgcat tatgtcattc gtaatatgtt gaagtaacga gtatttgctt gccggttatt
     4801 ttgcttccgg caagcaaatg aattacacaa tgcaagaggg ttatttgttc agcaaatgac
     4861 ccattttctc ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca
     4921 acggtacgcg ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt
     4981 tattggttaa caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag
     5041 tgaagtcgcg ctcatcagcg gcgaagccta actggtggtt agcctctacg gtatcgtaac
     5101 cttgatcctg cagtgcataa gcgcggattt tattcagcag accaatgtta cgaccttcct
     5161 gacggtgata cagcaaaata ccacggcctt cttcggcaat ttgcgtcagt gccgcttcga
     5221 gctggaagcc acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat
     5281 ggacgcgcgc aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat
     5341 cgtgtccggt tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca
     5401 gtttggcttc tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta
     5461 ttggcttatt ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac
     5521 ggaatggcac aaatctgtca atttttcctg gaactggcat tttcagttat gattgtggga
     5581 cttatcaaaa aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac
     5641 tattgcttgt tatgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac
     5701 aaagttggct actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg
     5761 tcattacaca tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg
     5821 ctgccattat gttatttgcc attctggcgg cggcaatcct tgtgggacaa ggagttaaat
     5881 cctggatcaa agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaatac
     5941 atcatattcc ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga
     6001 aagaacagtt ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcaaaaag
     6061 agacggggtt tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gctctgctgg
     6121 ccgttggttt gctgtggccg cgtcgtcgaa cgttaaccat tgctatcttg ttggtctggg
     6181 caacgggagt catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtgg
     6241 tagctacgtt gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt
     6301 gtgggccatt aacaccacct gtggaagaaa atcgcgaaat tgcacaacga gaacaagaaa
     6361 gttaacgctg gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc
     6421 catttcgcag acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa
     6481 tttattcgct ctaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg
     6541 gtgttagcga tcttcgtgat ttcggtcacg ttaggtgcgc agaacgatca acaggtgacg
     6601 tttaattatc tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt
     6661 gctgcggggt ttgctatcgg ttggctgatt tgcggtctgt tctggctgcg agttcgtgtt
     6721 tccctggcgc gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact
     6781 gacgtggctg tagcgccgca ttcgtcagcg gcgaaggaat aactttctat gctggagttg
     6841 ttgtttctgc ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg
     6901 caacaaaaca agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac
     6961 tttctgctta gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag
     7021 gatacgggta ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa
     7081 gttgatcgcg ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct gacctatgaa
     7141 cagcgtctgt tggcgattca acaactgggg cgtgattaca tggccgccgg gttatacgac
     7201 cgcgcggaag acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgcgctg
     7261 caacagttgc tacaaatcta ccaggctacc agcgagtggc agaaagcaat tgatgttgcc
     7321 gaacgcctgg tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt
     7381 gagttagccc tgcagcatat ggccagcgac gatctcgatc gtgccatggc cttgttaaaa
     7441 aaaggtgcgg cggcagataa aaacagcgcc cgcgtatcca tcatgatggg acgcgtattt
     7501 atggcgaaag gagaatacgc caaagccgtc gaaagtctgc aacgcgtgat atcccaggac
     7561 agagaactgg tcagcgaaac gctggaaatg ctgcaaacct gctaccagca gttgggtaaa
     7621 actgccgaat gggcagagtt cctgcagcgc gcggtggaag agaacaccgg tgccgatgct
     7681 gaactgatgc ttgcggacat catcgaagcg cgcgacggta gtgaggccgc acaggtctat
     7741 attacgcgtc agcttcagcg tcatccgacc atgcgtgtgt tccataagct tatggattac
     7801 cacttaaatg aagcggaaga agggcgtgcc aaagagagtc tgatggtgct gcgtgacatg
     7861 gttggcgaga aggtgcgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca
     7921 tacgctctct actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc
     7981 ggtcttgatg gcctgtaatt tttaaaaaaa tccgacttta gttacaacat actaattgtt
     8041 gatgttccat tgtgctccgg taactcaggc gcagaaaagc ctgccagggg agaaatcgca
     8101 actgttaatt ttttatttcc acgggtagaa tgctcgccgt ttacctgttt cgcgccactt
     8161 ccggtgccca tcatcaagaa ggtctggtca tgacgttaac tgcttcatct tcttcccgcg
     8221 ctgttacgaa ttctcctgtg gttgttgccc ttgattatca taatcgtgat gacgcgctgg
     8281 cctttgtcga caagatcgac ccacgcgatt gtcgtctgaa agtcggcaaa gagatgttta
     8341 cgctgtttgg acctcaattt gtacgcgaac ttcaacaacg gggttttgat atcttcctcg
     8401 acctgaaatt tcacgatatt cccaacactg cagcgcacgc tgtcgctgct gcagctgact
     8461 taggcgtgtg gatggtgaat gttcatgcct ccggtggggc gcgtatgatg accgcagcgc
     8521 gtgaggcact ggtgccgttt ggcaaagatg caccgctttt gattgctgtg acagtattga
     8581 ccagcatgga agccagcgac ctggccgatc ttggcgtgac actgtcacct gcagattatg
     8641 cagaacgtct ggcagcactg acgcaaaaat gtggccttga tggtgtggtg tgttctgctc
     8701 aggaagctgc gcgctttaaa caggtatttg gtcaggagtt caaactggtt acgccgggca
     8761 ttcgtccgca ggggagtgaa gctggtgacc agcgccgcat tatgacgcca gaacaggcgt
     8821 cggcggctgg tgttgattat atggtgattg gtcgcccgat aacgcaatcg gtagatccag
     8881 cgcagacgct gaaagcgatc aacgcctctt tacagcggag tgcatgatga gtgattccaa
     8941 cagccgtctg gtctactcaa cggaaaccgg acgtattgat gagccaaaag cggcccctgt
     9001 acgccctaaa ggcgacggtg tggtgcgtat tcagcgtcag accagtggac gtaaaggtaa
     9061 gggcgtttgc ttgattaccg gtgtcgatct cgatgatgcc gaactgacaa aacttgcagc
     9121 ggaactgaag aaaaaatgcg gctgcggcgg agcagttaaa gatggagtta ttgaaatcca
     9181 gggcgataag cgtgatttat taaaatcact acttgaagcg aaagggatga aggtaaaa
//
LOCUS       DAOTRK010000169         9225 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.170, whole genome shotgun sequence.
ACCESSION   DAOTRK010000169 DAOTRK010000000
VERSION     DAOTRK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.170"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..267
                     /locus_tag="QIE41_004079"
     CDS             <1..267
                     /locus_tag="QIE41_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDS3532894.1"
                     /translation="NRDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPA
                     WPAVWLERDDDTGFQQVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            527..928
                     /locus_tag="QIE41_004080"
     CDS             527..928
                     /locus_tag="QIE41_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532895.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            916..1323
                     /locus_tag="QIE41_004081"
     CDS             916..1323
                     /locus_tag="QIE41_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDS3532896.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPSCGTGVYSREGGQEGNWKDRN"
     gene            1607..3226
                     /locus_tag="QIE41_004082"
                     /pseudo
     CDS             1607..3226
                     /locus_tag="QIE41_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            3306..3598
                     /locus_tag="QIE41_004083"
                     /pseudo
     CDS             3306..3598
                     /locus_tag="QIE41_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(3638..4672)
                     /locus_tag="QIE41_004084"
     CDS             complement(3638..4672)
                     /locus_tag="QIE41_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HDS3532897.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(4675..5640)
                     /locus_tag="QIE41_004085"
     CDS             complement(4675..5640)
                     /locus_tag="QIE41_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDS3532898.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            complement(5697..6455)
                     /locus_tag="QIE41_004086"
     CDS             complement(5697..6455)
                     /locus_tag="QIE41_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDS3532899.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVMMLDGDDRVEKPE
                     GVIAQPSQPDHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(6469..7683)
                     /locus_tag="QIE41_004087"
     CDS             complement(6469..7683)
                     /locus_tag="QIE41_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDS3532900.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(7879..8239)
                     /locus_tag="QIE41_004088"
                     /pseudo
     CDS             complement(7879..8239)
                     /locus_tag="QIE41_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8301..8991
                     /locus_tag="QIE41_004089"
                     /pseudo
     CDS             8301..8991
                     /locus_tag="QIE41_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2484 a         2128 c         2246 g         2367 t
ORIGIN      
        1 aaccgggata cctggtgtcg tgccggtgat taccgtgctc cgggatcact ggccgggatg
       61 attaaacagg cgtggtgcag tgccctggga gtggatgtcg ggtgccatgc cacgctggtg
      121 cattttccgg cctggccggc ggtgtggctg gaacgcgatg atgacactgg ctttcagcag
      181 gtgctggaac gtgctgacta tctggcgaag gaacatacca aagctcactg caccggtgag
      241 cgcaattttg gctgtagccg gagttgagca cgactggcgc gccaataccc ggcaaaccaa
      301 aagtatgggt tgttgtctgg cggcaacgat gtatccactc gcccactgac tgttttttac
      361 gtcagtgttc cattgtgtga caaccacagt gctgtaacag caaccagcct gctttaccca
      421 aactccagtc cagattttaa ctgaaccgcc attcacaccc tgaccacgct gcccgtaccg
      481 ggacggctgt tgcctgcgtg tcgtctggcg gctgaggaat atgactatgt acgcaaaatc
      541 ctttatcgct cttgatggca acggacgtct gacgggcgcc cgtactgcac aggccgcacc
      601 ttatgctaac tacacctgcc acttgtgtgg cagtgcactc agataccatc cgcaatacga
      661 cactgaactt ccctggtttg aacacactga cgacaggctg acagagcacg gtcaacagtg
      721 cccttatgtc aggccggagc gcagagaaat acagttgatt aaacgtctgc agcaattcgt
      781 accggatgcc ttacccgtgg tgcgtaaagc cagctggcac tgcagacaat gtcaccacga
      841 ttattatggg gagcggtact gcacacactg ccagaccgga ggattcagta ttccccggac
      901 aactcaggag gaaatatgcg aattctgaac tgttatatgg cgaatgacag caaaggccat
      961 tttgttacgg cgaaagaagc agcgaagcac aaccgccagg acgttttgtg ctgtgtgtcc
     1021 tgtggatgcc cgttaacact tcagcggggc aatgacggac aaccaccgtg gtttgaacat
     1081 gaccagatga ctgtcgctga gaaaatcctg ctgcgatgca cctggcttga cccggcagag
     1141 aaagaggccc gtcgtttgca tctgcagggt atgacggttc cggattatac ggtgaaggtg
     1201 agaaagtggt tttgtgtgat gtgtgacgaa aattatgagg gggaaaagtg ctgcccgagt
     1261 tgtggtaccg gggtatacag cagggagggg gggcaggagg gcaactggaa ggataggaac
     1321 tgagcggttc agtcgcggaa atcagttgca gagctgtaat gatatctccg ggagtgctgc
     1381 gttggtaggg gcatcccggg atagagaaat tttagcactt tgccctgaat gattgtagac
     1441 gtcccgagta ctcgtaccgg tctgagttat tacagttaga tattcacttt ttctcccgca
     1501 tacttcacat ctgtcaacga cggatgaaaa gtggttcttc cgcaacctgg gtggaagaaa
     1561 atggatgaaa ctttctggcg aacaaggatc taagctgttt taagttatgg gcaacgcaat
     1621 gcactctctt aagacacttc tacagttacc ttgcggatgg cgatgcagtc gacaaattat
     1681 tagctctgac ggtataaccc tccatctcca cggaaaacgc aaaacagcac aatgtcctga
     1741 atgctttaag cgtagcgact ctgttcatag ttgtcgtcgg cgccggatac agcatctacc
     1801 ctgctcaggg cagacgctat ggcttgtatt ttccgtccgc cactggtact gccgtaaccc
     1861 ggcttgttca cgtaaaattt ttgccgagtc gcttgctccc ttcgccagtt cacaccagca
     1921 gtcttcacag gcgttacaaa attaacaatg tcaactggga ttaatagctg gaggtgaggc
     1981 tggaaaacgg gctgcaacgg cagtgggtct ccgttgcagt gctgatactc ttcttcgcag
     2041 gcttatcaat accccggaga cgaaacagtc aggcgcgcct catgtcggta ttgatgagtg
     2101 ggcgtggcat cggggccact gctgcggtat gttaatagtc aatcctgata ctcaccgtcc
     2161 cctcgtcctg cttcccggcc gtgatcagcg tacgctggcg acctggttca gaaaatatcc
     2221 ggaaatacag gttgtctcgc gtgatcgcag tggagtctat gcgacagcag cacgtgaagg
     2281 tgcacctcag gccagacagg tggccgatcg atggcacctg ctaaaaaata ttggtgatga
     2341 gcctgaacga atgatgtaca gacatatgcc tctgatacgt cttgttgtca gagagttatc
     2401 actgaagaaa tcacctgagc cagaaatatc tgtgcctgta gcatcgctcc gtcgtctgga
     2461 acgccttaaa cagcacatcc gcaaaaaacg gcatcagcgt tggacagagg ttatggccct
     2521 gcataacaag ggatgtagtt tcagggaaat atcccgtatt acaggcctgt cgcgagtgac
     2581 agtcagtcgc tgggtgggtt caggaacatt ccctgaaatg tcaaccaggc ctccaaagcg
     2641 agggcttctg gacccatgga gggagtggtt aaaagagcaa cgagaatgtg gtaattataa
     2701 ctccggccgg atatggcggg aaatggtggc caggggggtt acaggcagtg aaaccatcgt
     2761 cagggatgct gttgccaaat ggcataaagg ctggatccca ccggttacta ctgccgcaag
     2821 acttccttca gtgtcccggg taagccgctg gttgatgccc tggagaataa tcaggggtga
     2881 agaaaattat gctttccgat ttattagtct gatgtgtgaa aaagaaccgg agttgaaaat
     2941 agcgcagcaa ctggtactcg agttctaccg tattctgaaa acctaaaata aatcacagct
     3001 aagcagctgg ttcactcggg tccacgaaag cggctcagca gaacttcggt gcgtagctgc
     3061 ggggatggaa gctgatgctg cggctatatg tgaggcaatc agcagtcgct ggagtactgg
     3121 tgttgtcgaa ggtcatgtaa atcgcctgaa ggtgctgata cgccagatgt atggtcgagc
     3181 cggacttgaa ctacttaggc ggagggtaat gagtccactg gcatgaaaat cttcacctag
     3241 gttgaggaag agccactttt gcactgttcg tgacagtatg gcctttatgt actcactttg
     3301 ttaccggtag ctggatcatc actaccgtgc cgcaaatggt ggtgaacagc atcaattgtg
     3361 gaatgaatgt tgccgaagcg accaatgcgc ctcgtttcca ccatcagtgg ttgccggacg
     3421 agctgcgtgt tgaaaagggt ttagtccgga tacgctcaag ctgctggaag caaaaggtca
     3481 gaaagtggcg ctgaatgagg cgatgggcag cacgcaaagc attatggttg ggtcggatgg
     3541 tgagttgtac ggcgcatccg actcgcgctt ggtggatgat ttaacggcgg ggtattaatt
     3601 gacatccccc ggcgcaatgt gccgggggat aatggtttta tgcggtcaat aatcgtccgg
     3661 gattatcgat acagagtttt ttgaggatcg ttttatcgac gccgcgctcc gccagatagg
     3721 ccagaaaaac atctggaaca aatccccagc cattaccgcc atttttagcc cacatttgtt
     3781 taaggaagac atcgtggctg agaacaagct gatcggcgta tcccaactca atgagatgag
     3841 cgacggcatc ggcagtctcc tgcacccctg gcgctattcc ctctttcggg aaggtaatgt
     3901 ctaggccaat catgtcgaat tccagccaga cacctttatc aagcattttc cgctgatacg
     3961 ccacgtcttt tcctgacgga tccgagtgtg cgagagagac tttatttggc gagacgccca
     4021 tttcacctaa cacaatgtcg agtacttcat caccgcgacg aagccagccc ggcatgtgaa
     4081 tattcatcgc cacatgagga ttgttaatct gtgccagcga ggcagcccgc aagctgttat
     4141 gctcggcttc ggtaaatgtc ggtgagacac ctatttcacc gatcattccg gcacgaatat
     4201 ccgtatcgcc aatcccttga ttcaattctt tatcgatcgt tgtcgctagt tcatcaaccg
     4261 ttttatgaat tctctggctt tcaaattttt ccaggtaggg accggaggaa gcaacaatat
     4321 tcagacctgt ttttaatgcg acttctcgta atgcctgcgc atctcgtccg atagactcag
     4381 accctgtggc atcaacaatg gtgcgaccac cgagcgagat aaagttatta atttcgaaga
     4441 tcacatcttc aataggtttg cggtccatat tatcagcgca gcaataagga tcgtgtttta
     4501 atgcccactg gatttctgcc gtgacttttt tatcaacaag ccgttgtgaa aaaggatagc
     4561 aaggagcatc cacaacagag gaaagatcgt tgaacagatg ttcatgagga agtgttagcc
     4621 ccatatcttc acgggcgacg gggcccgtca cagtttgtag ataatctttc atgattatac
     4681 ctccattttc ttcattgttt tcgaggctct gtattccgga atagtggtaa caattgcacc
     4741 gaccagtgca aacagtgttc cgatgatcgt gaccagataa acggtattgc ccagcgatgg
     4801 aattaatacg tcaatcaata ctgatcccag taactggccc gccgttgaag caacccccag
     4861 catcagtaac ccaagacctc tgaccaaaat ggccattaaa ccgatagaca ttagtccaag
     4921 cgggccgcca agatacatcc accagacatc tggtaattga acagtgacgt gccctaatgc
     4981 gacacgaata gctagcgctg tgcctaaaac acaaaatcca acgatgaaat tccaggtgat
     5041 tgaaaccagc atggatcccg tagcttcagc tacttttgca tttccagcgg gctgccatcc
     5101 tgcaagcaaa ccagctaaga acggaagaat ggcaagtaaa atgaaagagg tagaatgcca
     5161 ctgcggtgac acaacaaaaa tcgtagcaat aaccgcaaat aatgcaccga tgatgcgata
     5221 aggggtaaag tattttttct cgtcgacacc gataccaaag cgatcgcaaa gtaaaccaga
     5281 cagcaatagc gcggagatca gtgcggtctg aaacgttgcg accccgagtg cgctggctga
     5341 tgcgccttca gaaaatacca ccatcgctcc acagagaccg gcaaaccagt tccagagagg
     5401 aacttttcgg tttttaatca gagaaggaat tgccgcaaat tgttgccggg tttcttttcg
     5461 cgccataata ataaaaaaca tcacgactaa accactggca aaggagataa ctgcgcatgc
     5521 attaccgtcc tgtaaccagt gacctaattg accattcacc gctgattgca taggggagag
     5581 catacccgca agaatcgtgg caagcatcca taagggagtt gagtacttat tctttttcat
     5641 acatcaatcc ttctattata gtaaatggag taactggtgt taccccatta ttattattat
     5701 cttgcaatat gagtcagtat atttacccac aacgcggtat agaacggcca ggacataaac
     5761 tgctgtgtac cccagtgcgg tgaacagtca ctcataaagc aggctatttt cccgttgtga
     5821 tagttaccaa agaccagtaa tggatcgttg tttattgtta ataccacctc agcattttct
     5881 ttagcgatcg cccggttata acctaggaaa aaaggatatt cagagaaccc tttaataacc
     5941 ggatggtcag gttgtgatgg ctgtgcaata acaccctctg gtttttcgac acgatcgtca
     6001 ccatctaaca tcataaccgg gagcacatca gccagaactg tatttttata attggctttt
     6061 gcttcaattc ccatgaatga gagatagccg ccaatcatca ataacccacc accgttattc
     6121 acatattctt taatcagttc gagagcgttt ggttttatgc gaagctgata aaaagtatca
     6181 ttttgtagca ggaaagtatt actaccaata tcactaataa cgatggcatc atattgttct
     6241 aactgtgcca catcttcagg gaaggcaatt tgtaccgtat gtgccggcat ataggtgaca
     6301 tctacctgac tatttttcag acattggagt aaccaggtcg caccttcttc atatttactt
     6361 gaggtaaaac tgtcatatcc ttttgaatga atcatatgaa tatgccagga ttctccaata
     6421 aagagcacct ttagtgattt tttggtgtta ttcattctct gtcccttctt aatgcgtaaa
     6481 aatagtttgc tgataatgta tactctgaat tcgatgtatc acagattcat gttcaggcat
     6541 gtcggaagca ttgcttgtct ccaccgccag ggatgcaaag gcactggcat aacacagtgc
     6601 tgattccagt gattttcctt tcgcaagtcc tgatgccaga gcgccgttaa aagcatcccc
     6661 ggctcctgcc gtatttttaa cgaccgctgg aaacgctggc gaatgaatga attttttgcc
     6721 atcaaatgcg agtgagccct tgctaccgag cgtgataacc gtgtttttta cccctttatt
     6781 gtggatagct tctgcagctc gtttagctga ttcaagatcg tgtacgtcta tgccggacag
     6841 taaaccagct tctgtttcat tgggggtgat ataatcaaca tcctgaataa gttcgttgac
     6901 gatatcatta tagggtgccg ggttaataat gaccgggata ctgttttttt gcgccaatgt
     6961 gatcgcttgt tgcagagcgg tataatttgt ttcgagctgc agaagtatga catcagaatt
     7021 aataatcgct tctttctgaa tagttatttc atcggacgac attgtcatgt tggcaccggg
     7081 atagatcgca ataacattat cgccagtacc ttcattaaca aggatcgtgg cagttccggt
     7141 ttgcgtttct ttcgtttgat aaattatact tttataaatc ttagacgaat tcataaagtt
     7201 gatcgcataa tcactaaagt gatctgtacc tacttttgtt atgaaataaa catcagtatc
     7261 ggcaaagctt gctgccagcg cttggttaca accttttccc cccggtgaaa atataaaatt
     7321 acttgccaga agcgattctc cgatagtggg aagcctcggc aaataactga tgatatcaac
     7381 attaaacgaa ccaataacgc atacttgact tttcatagtt gagtggcctt tttttattcc
     7441 ctgggctgta tcataatttt caagcgcata agcgatttca tttttcgcaa tagtatcaag
     7501 cgagtctagg acgataaaag caccgccatg acatcgagtc agatagccaa gtctggcaag
     7561 ttcattaaga tctgatcgaa tagtttcttt cgttacattg aggctattgg acagatcgga
     7621 tacttttaca gaaccgaatt tactgagtcg tttcaatatt tcatgatggc gttcaaattt
     7681 catgcttcac ctcacttcag ttacgttaat atctgtcgat gtatattgat gtgataatca
     7741 ccataaagtg gcggggaaaa gcaaaaaaaa tcaaaaatag ccggagaaaa ggtaagcgtt
     7801 aatggagaat catattgatg cttatttatt ggtgagaact acgttccatg gcaggagttc
     7861 atcaatacgg ttggaaggtt attccggcag tacgctcagg atatggcgca gatactcttc
     7921 cggatcaatg tcgttcagaa ggcaagtgcc gataagccca tacaacagtg cactgcgctc
     7981 gccgtcgaag aacataaagt ttttatttcc gagacagatg gcacaaaggg ctttttccgc
     8041 ggcattatgt ctgcctccgc ctgaccgtca acactgtaat aacagagtgc gccccactga
     8101 ttcagtacac agctgaacca ttctcccagt ctggattttt ggacagcgtg gcatttttct
     8161 ccaccatcca cgcatgcagt gagtccagta acagtttgct cttcctctgc ctgaccacaa
     8221 gatgttctgg ttctggcata ctgaatttgg ccacctgagc ataggtaata tgctcacctt
     8281 agaacaacac agatgctcca atgaaaaaag gaaatttcag cgcagagttt aaacgcgaat
     8341 ccactcaact agttgttaac cagaacttta cggtggcagg taccatccaa agctatggat
     8401 gtcggtcttt ccacaatgac aagataagtc aaacaactgc gtgatgagca tcagagcaaa
     8461 acaccaaaag cctctctgat gacaccagaa caaatcgaaa tacgtgagct gaggaaaaag
     8521 cggcaacgta ttgaaataga gaatgaaata ttagaaaagg ctacggcgct cttgatgtca
     8581 gactccctaa acagttctcg ataatcgaga agctcagagc gcattatctg gtgatcgcac
     8641 tttgccatgt gtgcaggatt catcgaagca gctacagaga ttggaaaaac cgtcctgaaa
     8701 aaaagacggt agacgggctg tattacgtag tcaggtaatt gagcttaatg gcggagcaag
     8761 gaacatcgcc acaattgcaa cccggagaga ctaccagatg ggacgcaagc ttgctggcag
     8821 tctcatgaaa gaacaggggc tggtcagctg tcagccgttg accctccggt acaggcatgg
     8881 cagtcatgaa cttgtagcta tcctaagaac ttgaagggcg cttagctgtg acacagctaa
     8941 atcaagtatg gtgacgtgac ctatatctgg actggtaaac gatggggata cccgcttacg
     9001 cgagagcaat aagtggctgg ccaccattct cgatacaaaa ctctgctgaa tagcagcgat
     9061 tttcccagcg tggtgcgtct tttgcatttt actggttctg agttatcaat tatctgttca
     9121 ctaatgacta ctctaaaaag ttgtatcaat ttaagtgata tagggagtct ggagattcag
     9181 ggggccagcc taggaacatt ggttacattt ttagtttcca aacat
//
LOCUS       DAOTRK010000170         9126 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.171, whole genome shotgun sequence.
ACCESSION   DAOTRK010000170 DAOTRK010000000
VERSION     DAOTRK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.171"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            180..335
                     /locus_tag="QIE41_004090"
     CDS             180..335
                     /locus_tag="QIE41_004090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532901.1"
                     /translation="MAALVVTWFNPVKEAFYMHLPAPGKTKKVALVDCMRKLLTILNA
                     MLRKNEE"
     gene            1146..2129
                     /locus_tag="QIE41_004091"
     CDS             1146..2129
                     /locus_tag="QIE41_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDS3532902.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQSAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKISATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEGSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            2201..3349
                     /locus_tag="QIE41_004092"
     CDS             2201..3349
                     /locus_tag="QIE41_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HDS3532903.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            3373..5049
                     /locus_tag="QIE41_004093"
     CDS             3373..5049
                     /locus_tag="QIE41_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HDS3532904.1"
                     /translation="MKLFKSILLIAACHAAQASAVIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            5059..5799
                     /gene="kdsB"
                     /locus_tag="QIE41_004094"
     CDS             5059..5799
                     /gene="kdsB"
                     /locus_tag="QIE41_004094"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDS3532905.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVVGV
                     AEVWVATDDQRVEKAVQAFGGKSIMTRNDHESGTDRLVEVMHKVEADININLQGDEPM
                     IRPRDVGTLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEI
                     AATGPGVDTPACLEKVRALRAQELAENA"
     gene            5796..7823
                     /locus_tag="QIE41_004095"
     CDS             5796..7823
                     /locus_tag="QIE41_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDS3532906.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQNVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNAALADQAREAMHTIVTGDLSKYNLAPAFVADESERADIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMEENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLQRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLCTATNRLSYSRRCTAATACVVWGVKGEQQWLAEAQQKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRRERRLTIADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            7858..9096
                     /locus_tag="QIE41_004096"
     CDS             7858..9096
                     /locus_tag="QIE41_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDS3532907.1"
                     /translation="MQGNALTILLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRALHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLDERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNGGYYGGV
                     KFENALGMNKDFLFGSKSIS"
BASE COUNT         2424 a         2156 c         2277 g         2269 t
ORIGIN      
        1 ctttatataa aataaacttg ggtgcataac gccatcaggt cttaaacact cccgaatcat
       61 caataaacat cccccgctaa caaaacacag ttcagttcac ataacatccc tgttatccca
      121 cacatatctg ctggccgaac gacgccagtg cgcgtacgga ggcaagagca gccctgcaca
      181 tggcagcgct tgtggtaacc tggtttaatc cggtgaaaga ggcgttttac atgcatctgc
      241 cagcacccgg aaaaaccaaa aaagtggcgc tggttgactg tatgcgtaaa ttgctgacca
      301 tcctgaacgc aatgctcaga aagaacgaag agtgaaatga atcgtagcat catgttgctc
      361 cataattttt acgttcaaga cagttgatta tcggcgcata tgactcaaaa agagcctgat
      421 gattgcctct tttccatagg ctctttctct gaagtgaagg aaggcccgga agaaagcact
      481 cgctggcata cgtgactcca gctcaaccgg agataatcac cattgttatt tttctattta
      541 ataataatct cctgaaaaat agatatagca aaaatacaat ccggccagat ttaattccgc
      601 attcattacc agaaataatc aacaccacaa ctatcatatc actcaatagt caccgctaaa
      661 attttattat tttcctcttc tttacataga aattgtcaac ctgtttatta tgaatcactt
      721 aaataattaa tcacaaaaca acatcaccat tatttgcgtg ttttatttat taataacata
      781 gtcatacgtt ctttttttac aaggaaataa tcaattgata aattgttttt ggcattaata
      841 gtcaagtagg aaacatttta ataaatgata aaaatcctaa attccttgtt cataatgtag
      901 gagtgtgtta aataggtggg caaaatttgg ttccctttct cgcctgtaat attgcagcgt
      961 cttcgcacct ccatgagaca ttgcgactta ataagaaggt gataagtcct gcatataagc
     1021 atggactgac catggtttta tattcattaa aaattttgtt acaacccatt gatttagcat
     1081 aaataaatta tagtgggttc aggtttgttg tgactgtggc attatttccg tgcaaaggag
     1141 ctgatatgtc tgaaagacat ttacctgatg accagagcag tactatcgat ccatatctaa
     1201 ttacctctgt tcgccagact ctggcagaac aaagcgcagc attacaaaac ttgtctaaac
     1261 aactggattc cgggcagtac cagcgtgtcc ttaatttgat aatgaactgt aaagggcacg
     1321 ttattctttc gggaatgggt aaatcagggc atgtcggtcg taaaatatca gcgacgctgg
     1381 cctctacggg tacgcctagt ttctttattc atcctgcaga agctttccat ggcgatctgg
     1441 gcatgattac gccttacgat cttctgatcc ttatttctgc cagcggtgaa acggatgaaa
     1501 tcctcaagct agttccttca ctgaaaaatt tcggcaatcg aattatcgcc attaccaata
     1561 atggaaattc cacgctggcg aaaaatgctg atgccgtgtt ggaactccac atggcgaatg
     1621 aaacctgccc gaataatctt gcaccaacaa cgtctaccac gctgacgatg gcgatcggcg
     1681 atgcgctggc gattgccatg atccaccaac gcaaatttat gccgaatgat tttgcgcgct
     1741 atcacccggg cggttcatta ggtcgtcgcc tgctgacccg cgttgctgat gtcatgcagc
     1801 atgatgttcc tgcggtacag ctggatgcgt catttaaaac cgtgattcaa cgtatcacca
     1861 gcggatgcca gggaatggtg atggtagaag acgcagaagg tgggctagcg ggcattatca
     1921 ccgacggtga cctgcgtcgt tttatggaaa aagagggttc tctgacatcc gctacggctg
     1981 cgcagatgat gacacgtgaa ccgctgacgc taccggaaga caccatgatc attgaagcgg
     2041 aagaaaaaat gcaaaagcac cgcgtctcaa cgttattggt gaccaacaag gcaaataaag
     2101 tcactggcct tgtgcgcatt ttcgactaat taagcaacgg ggttcgagag gttagtctct
     2161 cattgagttt gcgccccact gaaactttga taatcgttac atgttgataa aagtgaagtc
     2221 tgccgtatcc tggatgcgtg ctcgtctgtc tgccatctca ctggcagata tccaaaagca
     2281 cctggcaaaa atcatcattc tggcaccgat ggcggtgctg ctgatctatc tggctatctt
     2341 cagccagcct cgctatatga gcgagtcgaa agtcgccatt aaacgctcgg atgatttaaa
     2401 cagcggcagc ctgaattttg gtctgcttct gggtgcctct aaccccagtt ccgcagaaga
     2461 tgcgttgtat ctgaaagagt acatcaactc gccggatatg ctggcggcgc tggataagca
     2521 actaaatttt cgtgaagcgt ttagccacag cgggctcgat tttcttaatc atcttagcaa
     2581 ggatgaaacc gcagaaggct tcctgaagta ctacaaggac cgtatcaacg tctcgtatga
     2641 cgataaaacc ggattactga atattcagac gcagggcttt agcccggagt ttgcgcttaa
     2701 gtttaaccaa accgtgctga aagagtcaga gcgctttatc aatgagatgt cacatcgcat
     2761 cgcgcgtgac cagcttgcct ttgcagaaac ggagatggaa aaggcacgcc agcgtctgga
     2821 cgccagcaaa gcggaattgc tctcttatca ggacaacaac aacgttctgg atccacaggc
     2881 acaggcacag gcggcgagca cgttagtgaa tacgctgatg ggccagaaga tccagatgga
     2941 agcggacctg cggaacttgc tgacgtatct gcgtgaggac gccccgcaag ttgtgagtgc
     3001 gcgtaatgcg attcagtcat tgcaggcaca aattgacgaa gaaaaaagca aaatcactgc
     3061 gccacagggt gacaagctaa accgtatggc agtggatttt gaagaaatca aatcaaaagt
     3121 agagttcaac accgagctgt acaaactgac cctgacctcc attgaaaaga cccgtgtaga
     3181 agcggctcgt aagctcaagg tgctgtcagt gatcagttcg ccacagttgc cgcaggaatc
     3241 gtcttttcca aatatccctt atttgatcgc ctgctggtta ctggtgtgct gcttactgtt
     3301 cggcaccctg aaactgttgc tggctgttat tgaagatcac cgagactaac gctgtcgctg
     3361 aatgagtttg tgatgaaatt atttaaatca attttactga ttgccgcctg tcacgcggcg
     3421 caggccagcg cggtcattga tattaacgct gacccaaacc tgacaggagc cgcgccgctt
     3481 accggtattc tgaacggaca gaagtcggat acgcaaaaca tgagcggctt cgacaatacc
     3541 ccgccgcccg caccgccggt ggtaatgagc cgtatgtttg gtgctcaact tttcaacggc
     3601 accagcgcgg atagcggtgc gacggtagga ttcaaccctg actatattct gaatccgggt
     3661 gatagcattc aggttcgctt gtggggtgcg ttcacctttg atggtgcgtt acaggttgat
     3721 cccaaaggta atattttcct gccgaacgtt ggtccggtga aagttgctgg cgtcagtaat
     3781 agtcagctaa atgccctggt cacatccaaa gtgaaggaag tataccagtc caacgtcaac
     3841 gtctacgcct ccttattaca ggcgcagcca gtaaaagtgt acgtgaccgg atttgtgcgt
     3901 aatcctggtc tgtatggcgg tgtgacgtct gattcgttac tcaattatct gatcaaggct
     3961 ggcggcgttg atccagagcg cggaagttac gttgatattg tggtcaagcg cggtaaccgc
     4021 gtgcgctcca acgttaacct gtacgacttc ctgctgaacg gcaaactggg gctttcgcag
     4081 ttcgccgatg gtgacaccat catcgtcggg ccgcgtcagc atactttcag cgttcagggc
     4141 gatgtcttta acagctacga ctttgagttc cgcgaaagca gcattcccgt aacggaagcg
     4201 ttgagctggg cgcgccctaa gcctggcgcg actcacatta cgattatgcg taaacagggg
     4261 ctgcaaaaac gcagcgaata ctatccgatc agttctgcgc caggccgtat gttgcagaac
     4321 ggcgatacct taatcgtgag cactgaccgc tatgctggca ccattcaggt gcgggttgaa
     4381 ggcgcacact ccggtgaaca tgccatggta ctgccttatg gttccactat gcgtgcagtt
     4441 ctggaaaaag tccgcccgaa cagcatgtcg cagatgaacg cagttcagct ttatcgccca
     4501 tcagtggctc agcgtcagaa agagatgctg aatctctcgc tgcaaaagct ggaggaagca
     4561 tcactttctg cccagtcatc caccaaagaa gaagccagcc tgcgaatgca ggaagcacaa
     4621 ctgatcagcc gctttgtggc gaaagcacgc accgtggttc cgaaaggtga agtgatcctc
     4681 aacgaatcca atattgattc tgttctgctt gaagatggcg acgtcatcaa tattccggag
     4741 aaaacatcgc tggttatggt tcatggcgaa gtgctgttcc cgaacgcggt gagctggcag
     4801 aagggtatga ccaccgagga ttacatcgag aaatgtggtg gcctgacgca aaaatcgggt
     4861 aacgccagaa ttatcgtcat tcgtcagaac ggtgcagccg tcaacgcaga agatgtggat
     4921 tcactcaaac cgggcgatga gattatggtt ctgccgaaat atgaatcgaa aaatattgaa
     4981 gttacccgtg gtatttccac catcctctat cagctggcgg tgggtgcaaa agtgattctg
     5041 tctttgtaag gagttgaaat gagcaaagct gttattgtca tcccggctcg ttatggctca
     5101 tcgcgcctgc cgggtaagcc actgctcgat attgttggta aaccgatgat ccagcatgtt
     5161 tacgagcgtg cgttacaggt ggtgggcgtt gcggaagttt gggtggcaac agacgatcag
     5221 cgtgttgaaa aggccgtgca ggcatttggc gggaaatcca tcatgacgcg caacgatcat
     5281 gaatccggca ccgatcggct ggtcgaggtg atgcataaag tcgaggcaga tatcaacatt
     5341 aacctgcagg gcgacgaacc aatgattcgc ccacgggatg taggaacgct gttacaagga
     5401 atgcgtgatg accccgcgtt gccagtggca acgctatgcc acgcgatttc tgccgaagaa
     5461 gcgaccgagc caagcacggt aaaagtggtg gtaaataccc gccaggatgc gctttatttt
     5521 agccgttcgc cgattcccta tccgcgtaat gctgaaaaag cgcgctacct gaaacacgtt
     5581 ggtatttacg cttatcgtcg cgatgtgctg caaaactaca gccagttacc ggagtccatg
     5641 ccagagcagg cagaatcact ggagcagcta cggttaatga acgcggggat caacattcgc
     5701 acgtttgaga ttgccgcaac cggtccgggc gtcgacaccc cagcatgcct ggaaaaagtg
     5761 cgcgccctga gggcacagga actggctgaa aacgcatgat tggcatttac tcgcctggca
     5821 tctggcgtat tccgcatctg gagaaatttc tggcgcaacc atgccagaaa ctttctctgc
     5881 tgcgccctgt tccgcaaaac gtcgatgcta tcgccgtgtg gggacatcgt cccagcgcgg
     5941 cgaaaccagt cgccatcgcc aaagcagcgg gaaaacccgt cattcgtctg gaagatggat
     6001 ttgtgcgttc gctggatctt ggcgtcaatg gcgagccgcc gctttctctg gtggtggatg
     6061 attgtggcat ttactacgat gccagcaagc cttcggcgct ggagaaactg gtacaggata
     6121 aagccggaaa tgcggcattg gctgatcagg ccagagaagc gatgcacacc atcgtgaccg
     6181 gggatttgtc gaaatataac ctggcgcctg catttgtggc tgatgagtca gaacgtgcag
     6241 acatcgttct ggttgtcgat cagacattta atgacatgtc cgtgacgtat ggcaatgctg
     6301 gcccgcatga gtttgctgcc atgctggaag ctgcgatgga ggaaaatcct caagccgaaa
     6361 tttgggtgaa ggtgcatccg gatgtcctgg aaggaaagaa aacaggttat ttcgctgatc
     6421 tgcgtgccac gcaacgagta cgtttgattg ccgagaatgt cagcccgcag tcgctattgc
     6481 gacacgtttc ccgggtttac gtcgtgacat cccaatacgg ctttgaagcc ttgctggcag
     6541 gaaaaccagt aacatgtttc ggccagccct ggtatgcagg ctggggctta accgacgatc
     6601 gccatccgca gtccgctttg ttatctgccc gacgcggttc tgccacgctg gaagaacttt
     6661 ttgccgctgc atacctgcgt tactgtcgct atatcgaccc gctcacgggg gccgcaagcg
     6721 atctgtttac cgtactgcaa tggctgcaat tacagcgcaa ccatcagcaa cagcgtaatg
     6781 gctatttatg ggcgccaggc ttaacgctgt ggaagtcggc aatcctgaaa cctttcttgt
     6841 gtaccgcaac aaacagactg agttattcac gccgttgtac tgcggcgacg gcttgcgtgg
     6901 tatggggtgt aaagggagaa cagcaatggc tagccgaagc gcaacaaaaa tcactgccgt
     6961 tatggcgaat ggaagatggt tttctgcgtt catccggact tggctctgac cttttgccgc
     7021 cgctatcgtt ggtactggat aaacgcggga tctactatga cgccacgcgc cccagcgacc
     7081 tggaagtcct gcttaatcac agccagttaa cgctggcgca gcagatgcga gctgaaaaat
     7141 tacgccagcg actggttgaa agcaaactga gcaaatacaa cctgggggcc gatttctctc
     7201 tgcctgccga ggccaaagat aaaaaaatca tcctggtgcc gggtcaggta gaagacgatg
     7261 cctcaattaa aacaggcact gtgtcgatta agagcaacct tgagttatta cgcacagtac
     7321 gcgagcgcaa tccgcacgct tacattattt ataaaccgca cccggatgta ctggtgggga
     7381 atcgcaaggg caatattccg acagaactaa ttgctgaact cgctgattat caggcactgg
     7441 acgcagatat tattcaatgc atccagcgcg cagatgaagt gcacaccatg acatcattgt
     7501 ccgggtttga agcgttatta catggcaagc aagttcattg ttacggcctg cccttctatg
     7561 ccggttgggg tttaaccgtt gatgaacatc attgcccgcg ccgcgagcgc agattaacga
     7621 tagcagactt gatctatcag gcgttgattg tttacccaac atatattcac ccaacacggc
     7681 tacagcctat tacggttgag gaagctgcgg aatatttaat ccagacgccg cgtaagccga
     7741 tgtttattac ccgaaaaaaa gcggggcggg taatacgcta ttaccgcaaa ttaattatgt
     7801 tctgcaaggt cagatttggc taaacaattt catagttgac tattacacag attatttatg
     7861 caaggcaacg cactaaccat tttattatcg ggtaaaaaat atctgctatt gcagggaccg
     7921 atgggacctt ttttcaatga cgtcgccgaa tggttagagt cattaggccg taacgctgtg
     7981 aatgttgtct tcaacggcgg ggatcgtttt tactgccgcc atcgacaata cctggcttac
     8041 taccaaacgc cgaaagagtt tcccggatgg ttacgggctc tccaccggca atatgacttt
     8101 gataccatcc tctgctttgg tgactgccgc ccattgcaca aagaagcaaa acgctgggca
     8161 aagtcgaaag ggatccgctt tctggcattt gaggaaggat atttacgccc gcaatttata
     8221 accgttgaag aaggcggagt gaacgcatat tcctcgctac cgcgcgatcc ggatttttat
     8281 cgtaagttgc cagatatgcc tgcgccgcac gttgagaact taaaaccttc aacgatgaaa
     8341 cgcattggcc atgcgatgtg gtattacctg atgggctggc attaccgtca tgagttccct
     8401 cgctaccgcc accacaaatc attttccccc tggtatgaag cacgttgctg ggttcgtgcg
     8461 tactggcgca agcaacttta caaggtaaca cagcgcaagg tattgccgag gttaatgaat
     8521 gagctggatc agcgttatta tcttgccgtt ttgcaggtgt ataacgatag ccagattcgt
     8581 aaccacagta attataacga tgtgcgtgac tatattaatg aagtcatgta ctcattttca
     8641 cgtaaagcgc cgaaagaaag ttatttggtg atcaaacatc atccgatgga tcgtggtcac
     8701 agactctatc gaccattaat taagcgattg agtaaggaat atggcttaga tgagcgagtc
     8761 atttatgtgc acgatctccc gatgccggaa ttattacgcc atgcaaaagc ggtagtgacg
     8821 attaacagta cggcggggat ctctgcgctg attcataaca aaccactcaa agtgatgggc
     8881 aatgccctgt acgacatcaa aggcttgacg tatcaagggc atttgcacca gttctggcag
     8941 gccgatttta aaccggacat gaaactgttt aagaagtttc gggggtattt attggtgaag
     9001 acgcaggtta atgggggtta ttatggagga gtaaaatttg agaatgcatt gggtatgaat
     9061 aaagattttt tgtttggatc aaagtcaata tcataattta ttctaaatta tcaacgtaga
     9121 ataaaa
//
LOCUS       DAOTRK010000171         9042 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.172, whole genome shotgun sequence.
ACCESSION   DAOTRK010000171 DAOTRK010000000
VERSION     DAOTRK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.172"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(34..357)
                     /locus_tag="QIE41_004097"
     CDS             complement(34..357)
                     /locus_tag="QIE41_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDS3532908.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVGEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNMEFTLSYQCHHSA
                     FPVFL"
     gene            620..706
                     /gene="azuC"
                     /locus_tag="QIE41_004098"
     CDS             620..706
                     /gene="azuC"
                     /locus_tag="QIE41_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDS3532909.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            821..1072
                     /locus_tag="QIE41_004099"
     CDS             821..1072
                     /locus_tag="QIE41_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDS3532910.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1152..1655)
                     /locus_tag="QIE41_004100"
     CDS             complement(1152..1655)
                     /locus_tag="QIE41_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDS3532911.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTANFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            2451..3440
                     /gene="araF"
                     /locus_tag="QIE41_004101"
     CDS             2451..3440
                     /gene="araF"
                     /locus_tag="QIE41_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDS3532912.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3510..5024
                     /gene="araG"
                     /locus_tag="QIE41_004102"
     CDS             3510..5024
                     /gene="araG"
                     /locus_tag="QIE41_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDS3532913.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            5039..6025
                     /gene="araH"
                     /locus_tag="QIE41_004103"
     CDS             5039..6025
                     /gene="araH"
                     /locus_tag="QIE41_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDS3532914.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLVIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            6192..6992
                     /gene="otsB"
                     /locus_tag="QIE41_004104"
     CDS             6192..6992
                     /gene="otsB"
                     /locus_tag="QIE41_004104"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDS3532915.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            6967..8391
                     /gene="otsA"
                     /locus_tag="QIE41_004105"
     CDS             6967..8391
                     /gene="otsA"
                     /locus_tag="QIE41_004105"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDS3532916.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(8398..8826)
                     /gene="uspC"
                     /locus_tag="QIE41_004106"
     CDS             complement(8398..8826)
                     /gene="uspC"
                     /locus_tag="QIE41_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDS3532917.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         2358 a         2072 c         2279 g         2333 t
ORIGIN      
        1 aatcagcgaa gatcccctcg ctgattaaaa catttagaga aagacaggga aggcggaatg
       61 atggcactga tatgacaaag tgaattccat attcaaacat aaggaacccg caaaaaggct
      121 acagtttcct actggctgac caaaggggat cgcgtgggta tagccttcct gctggcatgc
      181 gcgatcggcg atcccgcgac tgacataacg atcggtatgg gcatgctgca aaaaagcctg
      241 atcataaacc aaccgaacaa tacctgttgc cgcgtcaact tcgcccacat gagcctggcg
      301 agtaactgtg caccccgcca gcgttattaa tgaaagagtc agaatcagaa gtctcatggt
      361 ccagccagaa gagtaaaaga aagcactatt ttacgcgtta cagcgctgcg acaatggccg
      421 gaaaagacgc ctggcaggcg ctatttctcg ccatctgtat gtgtcagaaa tgggggggct
      481 gctttttgat tatcattttt ataacattaa aggggtattt atgtgatttg catcacataa
      541 agattgattt ttgtgatggt tgtcatatta taagtgcaaa gacaataaca cctgcataac
      601 aaataatcgg agtgccgcga tgaaactgcg caagatcctg aaaagtatgt tcaataacta
      661 ttgcaaaacg ttcaaagacg taccaccagg caatatgttc cgatagcaaa aaacctgctc
      721 cggcaggttt ttttgtgtcc tgatgacggt ggatgtgact atcgctgaag atgtgaagcc
      781 gctactataa cggctttcaa aatgctatca ggagtttacg atgtcccagc cgctgaatgc
      841 cgatcaggaa ctggtttctg atgtggttgc ttgccagttg gttatcaaac aaattctgga
      901 tgttctcgat gtaatcgcgc ccgttgaagt tcgtgaaaaa atgtccagcc agctgaagaa
      961 tatcgacttt acgcaccatc ccgccgccgc agacccggtc accatgcggg caatccaaaa
     1021 agccatcgcg ctgattgaac tcaagtttac cccacagggc gaatcccatt aaaaaaaccg
     1081 ctgcttcaaa aagatgaggc agcggtttct caaaaacttc atcggcgcaa tgcattagcg
     1141 ccgatgtatg atcaatgcag ctgatgcgac acgacattca gaacatgttg atcggtctgc
     1201 acagggcaca tacccgcaag tttcgcactg cgcacttcat caagaatggt ttgcagcaac
     1261 aggccatcat gctgctgttc tttttccaga tcgcgcagga aattggcggt tgaatcatca
     1321 ttcagttctt tcgcttcatc ggctaactgt gccaacgtac tagaacgttg ctcgtattct
     1381 tccatcgttt tttggaaaag ttcttccagt gagttcagtt tttcaccggg aacatcaatg
     1441 gctttaacga tgggggtagc gccgacactc ttcataaagt taaacatgcg catcatttgg
     1501 gtcacattac tctgtgcctg ggcgcgaagg aaagtggcgg tgccgttcag actctgttca
     1561 gaacaccagt tactcaggtg aaggtagaga ttggatgcgt aaaactcgcg gttcatttga
     1621 gagttgagtt tgagaagcat tccagcggtt gccataccta atatccttat atccagaagt
     1681 ggagaggtgc aagataaatc aaaacaccat aattactgtt tgcagaataa ttcctttctg
     1741 ccggtccgaa atgtgatcgt ggagtcaatt ctgacgatga tctgaaataa gaataacgtc
     1801 tgaatgggta taagaaaatg cttaaatcat caagtttata tatttttaca tttcattgat
     1861 gaaatcaatg taactgcaat gaattttaat gatagtgctt tttgtatctt gttgatatta
     1921 atcggtgaat ttgaattcat gatgaagcaa tgatttttta aaacgttttt tcatttttgc
     1981 gatatggctc tcggtatacg tgttctgtta attaatgagc acggtgataa ttaatggcga
     2041 aatgaatgac gcgctttttc cacatctttg agttgcggtt attacaccct ttcaaaaact
     2101 caacgccagg taatgtggcc tattgactgg ttaaaaaaaa gacatcccgc tgggtaccaa
     2161 agacaacaag gatttccagg ctaatcttat ggattaatct gctgtgcact agacaatttg
     2221 tcagacaaat tggcttttcc ttatgtcttt tcccgctaaa tttatgcacg ttctcactgt
     2281 aattctgcga tgtgatattg ctctcctatg gagaattaat ttctcgctaa aactatgtca
     2341 acacagtcac ttatctttta gttaaaaggt aatgctttgt tttccgatta atttaacgaa
     2401 tgtcattcgt ttttgcccta cacaaaacga cactaaagct ggagagaacc atgcacaaat
     2461 ttactaaagc cctggcagcc attggtctgg cagccgttat gtcacaatcc gctatggcgg
     2521 agaacctgaa gctcggtttc ctggtgaagc aaccggaaga gccgtggttc cagaccgaat
     2581 ggaagtttgc cgataaagct gggaaggatt tagggtttga agttattaag attgccgtgc
     2641 cggatggcga aaaaacattg aacgcgatcg acagcctggc tgccagtggc gcaaaaggtt
     2701 tcgttatttg tactccggac ccgaaactcg gctctgccat cgtggcgaaa gcgcgtggct
     2761 acgatatgaa agtcattgcc gtggatgacc agtttgttaa cgccaaaggc aagccaatgg
     2821 ataccgttcc gctggtgatg atggcagcga ccaaaattgg cgaacgtcag ggccaggaac
     2881 tgtataaaga gatgcagaaa cgtggctggg atgtcaaaga aagcgcggtg atggcgatta
     2941 ccgccaacga actggatacg gctcgtcgcc gtactacggg gtctatggat gcgctgaaag
     3001 ctgcaggatt cccggaaaaa caaatttatc aggtacctac caaatctaac gacatcccgg
     3061 gggcatttga cgctgccaac tcaatgctgg ttcaacatcc ggaagttaaa cattggctga
     3121 tcgtcggtat gaacgacagc accgtacttg gcggcgtacg cgcgacggaa ggtcagggct
     3181 ttaaagcggc cgatatcatc ggtatcggta ttaacggtgt ggatgcggtg agcgaactgt
     3241 ctaaggcaca ggcaaccggc ttctacggtt ccctgctgcc aagcccggac gtacatggct
     3301 ataaatccag cgaaatgctt tacaactggg tagcaaaaga cgttgaaccg ccaaaattta
     3361 ccgaagttac cgacgtggta ttgatcacgc gtgacaactt taaagaagaa ctggagaaaa
     3421 aaggtttagg cggtaagtaa tttgccagaa aaattcccct ctgcatgatg cagagggggt
     3481 gtgaacgacc agtgattcac ggagacgtta tgcaacagtc taccccgtat ctctcatttc
     3541 gcggcatcgg taaaacgttt cccggcgtta aggcgctgac ggatattagt tttgattgct
     3601 atgccggtca ggttcatgcg ttgatgggtg aaaatggcgc aggaaaatca actctcttaa
     3661 aaatcctcag cggcaactat gcgccaacca cgggttctgt agtgattaat gggcaggaaa
     3721 tgtccttttc cgacacgacc gcagcactta acgcgggtgt ggcgattatt taccaggaac
     3781 tgcatctcgt gccggaaatg accgtcgcgg aaaacatcta tctcggccag ctgccgcata
     3841 aaggcggcat tgtgaatcgc tcattgctga attatgaggc gggtttacaa cttaaacatc
     3901 ttggtatgga tattgacccg gacacgccgc tgaaatatct ctccattggt cagtggcaga
     3961 tggttgaaat cgccaaagcg ctggcgcgta acgccaaaat tatcgccttt gatgagccaa
     4021 ccagctccct ctctgcccgt gaaatcgaca atcttttccg cgttattcgt gaactgcgaa
     4081 aagaggggcg ggtgatctta tacgtttctc accgtatgga agaaatattt gccctcagcg
     4141 atgccatcac cgtctttaaa gatgggcgtt atgtcaaaac ctttactgat atgcagcagg
     4201 ttgaccacga cgccctggtg caggcgatgg tcgggcgcga catcggtgat atctacggct
     4261 ggcaaccgcg tagttatggc gaggagcgcc tgcgtcttga tgctgtgaaa gcaccaggcg
     4321 tgcgtacgcc aataagtctg gcggttcgca gtggagaaat tgtcggtctg tttggtctgg
     4381 tcggggcagg gcgtagcgaa ttaatgaaag gcatgtttgg cggtacgcaa atcaccgccg
     4441 gtcaggttta tatcgaccaa cagccgatcg atattcgtaa accgagccac gctattaccg
     4501 caggcatgat gctctgcccg gaagatcgca aagcggaagg cattattcct gtgcactccg
     4561 ttcgcgacaa tatcaacatc agtgccagac gcaaacatgt gctcggcggt tgtgtaatca
     4621 acaacggttg ggaagaaaac aatgccgatc accacattcg ttcgctcaac atcaaaacgc
     4681 cgggcgctga gcaactgatt atgaatctct caggcggaaa tcagcaaaaa gccattctgg
     4741 gccgctggtt atcggaagag atgaaggtca ttttgctgga tgaacctacg cgcggcattg
     4801 atgttggcgc taagcacgaa atatataacg taatttatgc gctggcggcg cagggtgtgg
     4861 cggtgctgtt tgcctccagc gacttacctg aagtcctcgg cgttgccgac cggattgtgg
     4921 tgatgcggga aggtgaaatc gccggtgaat tgttacacga gcaggcagat gagcgtcagg
     4981 cactgagcct tgcgatgcct aaagtcagcc aggctgttgc ctgagtaagg agagtatgat
     5041 gtcttctgtt tctacatcgg ggtctggcgc acctaagtcg tcattcagct tcgggcgtat
     5101 ctgggatcag tacggcatgc tggtggtgtt tgcggtgctc tttatcgcct gtgccatttt
     5161 tgtcccaaat tttgccacct tcattaatat gaaagggttg ggcctggcaa tttccatgtc
     5221 ggggatggtg gcttgtggca tgttgttctg ccttgcttcc ggtgactttg acctttctgt
     5281 cgcctccgta attgcctgtg cgggtgtcac cacggcggtg gttatcaacc tgactgaaag
     5341 cctgtggatt ggcgtggcag cgggtttgtt gctgggtgtt ctctgtggcc tggtcaatgg
     5401 ctttgttatc gccaaactga aaataaatgc tctgatcacg acactggcaa cgatgcagat
     5461 tgttcgaggt ctggcgtaca tcatttcaga cggtaaagcg gtcggtatcg aagatgaaag
     5521 cttctttgcc cttggttacg ccaactggtt cggtctgcct gcgccaatct ggctcaccgt
     5581 cgcgtgtctg gttatctttg gtttgctgct gaataaaacc acctttgggc gtaacaccct
     5641 ggcgattggc gggaacgaag aggctgcgcg tctggcgggt gtaccggttg ttcgcaccaa
     5701 aattattatc tttgttctct ctggtctggt atcagcgata gccggaatta ttctggcttc
     5761 acgtatgacc agtgggcagc caatgacgtc gattggttat gagctgattg ttatctccgc
     5821 ctgcgtttta ggtggcgttt ctctgaaagg tggcatcgga aaaatctcat atgtggtggc
     5881 gggtatctta attttaggca ccgtggaaaa cgccatgaac ctgcttaata tttctccttt
     5941 cgcgcaatac gtggttcgcg gcttaatcct gctggcagcg gtgatcttcg accgttacaa
     6001 gcaaaaagcg aaacgcactg tctgatgctt ttttccacaa caatttaacg ttttttccca
     6061 ccacagccag ccgccacaac ggttggctgt tcttcgttac aaatggcgac ccccgtcaca
     6121 ctgtctatac ttacatgtct gtaaagcgcg ttctgcgcaa cacaataaga aaagagaagg
     6181 aggagaaccg ggtgacagaa ccgttaaccg aaacccctga actatccgcg aaatatgcct
     6241 ggttttttga tctcgatgga acgctggcgg aaatcaaacc gcatcccgat caggtcgtcg
     6301 tgcctgacaa tattctgcaa ggacttcagc tactggcaac cgcaagtgat ggtgcattgg
     6361 cattgatatc agggcgctca atggtggagc ttgacgcact ggcaaaacct tatcgcttcc
     6421 cattagcggg cgtgcatggg gcggagcgcc gtgacatcaa tggtaaaacg catatcgttc
     6481 atctgccgga tgcgattgcg cgtgatatta gcgtgcaact gcatacggct atcgcccagt
     6541 ttcccggtgc ggagctggag acgaaaggga tggcctttgc gctgcattat cgtcaggctc
     6601 cgcagcatga agacgcatta atgacattag cgcaacgtat tactcagatc tggccacaaa
     6661 tggcgttaca gcagggaaag tgtgttgtcg agatcaaacc gcgaggcacc agtaaaggtg
     6721 aggcgattgc tgcctttatg caggaagctc cctttatcgg gcgaacgcct gtatttctgg
     6781 gcgatgattt aaccgatgaa tctggcttcg cagtcgttaa caggctgggc ggaatgtcag
     6841 taaaaattgg cacaggcgca actcaggcat catggcgact ggcgggcgta ccggatgtct
     6901 ggagctggct tgaaatgata accaccgcat tacaacaaaa aagagaaaat aacaggagtg
     6961 atgactatga gtcgtttagt cgtagtatct aaccggattg caccaccaga tgagcacgcc
     7021 gccagtgccg gtggccttgc cgttggcatt ctgggggcac tgaaagccgc tggtggactg
     7081 tggtttggct ggagtggcga aacagggaat gaggatcagc cgctaaaaaa ggtgaaaaag
     7141 ggtaacatta cgtgggcctc ttttaacctc agcgaacagg accttgacga atactacaac
     7201 caattctcca atgccgttct ctggcctgct tttcattatc gcctcgatct ggtgcaattt
     7261 cagcgtcctg cctgggacgg ctatctacgc gtaaatgcgt tgctggcaga taaattattg
     7321 ccgctgttgc aagatgatga cattatctgg atccacgatt atcacctgtt gccatttgcg
     7381 catgaattac gcaaacgggg agtgaataat cgcattggtt tctttctgca tattcctttc
     7441 ccgacaccgg aaatcttcaa cgcgctgccg acatatgaca ccttgcttga acagctttgt
     7501 gattatgatt tgctgggttt ccagacagaa aacgatcgtc tggcgttcct ggattgcctt
     7561 tctaacctga cccgcgtcac gacgcgtagc gcaaaaagcc atacggcgtg gggcaaagcg
     7621 tttcgaacgg aagtttaccc gatcggcatt gagccgaaag aaatagccaa acaggctgcc
     7681 gggccactgc cgccaaaact ggcgcaactc aaagcggaac tgaaaaacgt acaaaatatc
     7741 ttttctgtcg aacggctgga ttattccaaa ggtttgccgg agcgttttct cgcctatgaa
     7801 gcgttactgg aaaaatatcc gcaacatcac ggtaaaattc gttataccca gatcgcgcca
     7861 acgtcgcgtg gtgatgtgca agcctatcag gatattcgtc atcagctcga aaatgaagcc
     7921 ggacgaatta atggtaaata tgggcaatta ggctggacgc cgctttatta tttgaatcag
     7981 cattttgacc gtaaattact gatgaaaata ttccgctact ctgacgtggg cttagtgacg
     8041 ccactgcgtg acgggatgaa cctggtagca aaagagtatg ttgccgctca ggacccagcc
     8101 aacccgggcg ttcttgttct ttcgcaattt gcgggagcag caaacgagtt aacgtcggcg
     8161 ttaattgtta atccctacga tcgtgacgaa gttgcagctg cgctggatcg tgccttgact
     8221 atgtcgctgg cggaacgtat ttcccgtcat gcagagatgc tggacgttat cgtgaaaaac
     8281 gatattaacc actggcagga gtgcttcatt agcgacctaa agcagatagt tccgcgcagc
     8341 gcggaaagcc agcagcgcga taaagttgct acctttccta agcttgcgta agtgcggtta
     8401 atctcccgta agtggaacta acagcacatc gacctcactt gaggcaatca ctcttttcgc
     8461 cgagcaggat gctcgcgaaa agaaactgtg attgtgatta ccgcaaatca ccaaatcgaa
     8521 atgatatttg cgacaaactt ccagaatatg ttcgcttaac tcaccgtagg caataaacgt
     8581 cttgtcaacg ggataccccg catcctgaat taacttatca agaaagcttt gcgtttcttc
     8641 ctgcatcacg ctgcgtaaat cttcaagcat cggcgcagct aattgattgt acatttccgg
     8701 gtcggaagcg agagtaatta aactgatgtg tccctttact ggcctggcga tagataccgc
     8761 ttttgccagc agttgctgac tttccggtgt aaccgcaacg gcgacaagaa tattgctata
     8821 gctcataacc tgttccttat tctgtgaact tcaggtgaca ttaaagcttt actcctaatg
     8881 gagaaacgac gcaatcccaa ctcagtcaaa caggtaaata ttagtctgat tattagtaaa
     8941 gacaattaat caatcactct cggtgatcat atgatttcaa ctgtaattat aagaaaataa
     9001 tgtactgatt ctccatatta aacaagtggt taatatatgt aa
//
LOCUS       DAOTRK010000172         9025 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.173, whole genome shotgun sequence.
ACCESSION   DAOTRK010000172 DAOTRK010000000
VERSION     DAOTRK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.173"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(148..864)
                     /locus_tag="QIE41_004107"
     CDS             complement(148..864)
                     /locus_tag="QIE41_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3532918.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDCHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGINNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            914..1129
                     /locus_tag="QIE41_004108"
     CDS             914..1129
                     /locus_tag="QIE41_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS3532919.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNSELKYNPELYKKQDSTSNEGKNDS"
     gene            1126..1551
                     /locus_tag="QIE41_004109"
     CDS             1126..1551
                     /locus_tag="QIE41_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDS3532920.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            1574..2536
                     /locus_tag="QIE41_004110"
     CDS             1574..2536
                     /locus_tag="QIE41_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3532921.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            2543..3289
                     /locus_tag="QIE41_004111"
     CDS             2543..3289
                     /locus_tag="QIE41_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS3532922.1"
                     /translation="MKNIAAVGVLERIRRLAPQGSVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTRKNHLAAAMGNRLMVKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            3311..4027
                     /locus_tag="QIE41_004112"
     CDS             3311..4027
                     /locus_tag="QIE41_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HDS3532923.1"
                     /translation="METVSDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVQEMM
                     AQEEMKTEIVEDIVRLQPSITEMKADDLILPSLHVANRELRRAKSDVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            4060..4341
                     /locus_tag="QIE41_004113"
     CDS             4060..4341
                     /locus_tag="QIE41_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDS3532924.1"
                     /translation="MILRQCAGTMRVESIGYLIGRSESAVRTKARELGISMILRGDFH
                     PSAKYSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQE"
     gene            4338..4640
                     /locus_tag="QIE41_004114"
     CDS             4338..4640
                     /locus_tag="QIE41_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HDS3532925.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEDALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGARAELALAEKLGHAVIFQEVGSEY"
     gene            4630..4910
                     /locus_tag="QIE41_004115"
                     /pseudo
     CDS             4630..4910
                     /locus_tag="QIE41_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309276.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
     gene            4897..5520
                     /locus_tag="QIE41_004116"
     CDS             4897..5520
                     /locus_tag="QIE41_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDS3532926.1"
                     /translation="MSEINYQVLREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAERHVNCLIETDAQAYANAEFIAEANPATVLALLDEQERNQQYIKRRDQEN
                     EDIALTVGKLLVELEAAKSKLNEQREYYEGVISDGCKRIAELEAREVQLPTRYDLRYG
                     HPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            5498..5854
                     /locus_tag="QIE41_004117"
     CDS             5498..5854
                     /locus_tag="QIE41_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532927.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            5948..6130
                     /locus_tag="QIE41_004118"
     CDS             5948..6130
                     /locus_tag="QIE41_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532928.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            6123..6299
                     /locus_tag="QIE41_004119"
     CDS             6123..6299
                     /locus_tag="QIE41_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532929.1"
                     /translation="MADFGSTKYNVSFEAWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            6296..6610
                     /locus_tag="QIE41_004120"
     CDS             6296..6610
                     /locus_tag="QIE41_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532930.1"
                     /translation="MSEINYQEGHETAGQAKPVAWRYRYVKKGVTDFQENLWVGDWKY
                     VPTKEDCNDRPNYEIQALFAAPPVPVTSEELVKAVHFYEQLKRENPPASGNLITDSQI
                     RQ"
     gene            7219..7374
                     /gene="hokD"
                     /locus_tag="QIE41_004121"
     CDS             7219..7374
                     /gene="hokD"
                     /locus_tag="QIE41_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDS3532931.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            7542..7814
                     /locus_tag="QIE41_004122"
     CDS             7542..7814
                     /locus_tag="QIE41_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532932.1"
                     /translation="MAHKTKFHNSDTDLAIRHERRSHAFKTDWYQHPPCTEEQAEWLI
                     HSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            7816..8874
                     /locus_tag="QIE41_004123"
     CDS             7816..8874
                     /locus_tag="QIE41_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDS3532933.1"
                     /translation="MRVLLRPVLVPELRLVVLKPGRESIQIFHNPRVLVEPEPKSMCG
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLCDQTSESLEQLAHQNLSAWMIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVADALPESVLCRSLGLPVEKIRSVYRESDIVPGEQTATSILKQR
                     TKNIALPLHVHQQQPPLQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            8875..>9025
                     /locus_tag="QIE41_004124"
     CDS             8875..>9025
                     /locus_tag="QIE41_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532934.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKL"
BASE COUNT         2470 a         1939 c         2491 g         2125 t
ORIGIN      
        1 ggtaactcga agtatttccc tgcgttctgt ggggcggggt gggttggtat gttgttaagg
       61 taacaagagt tacctttcga gtcaatacaa tgttgcaaaa ggtacatttg agggcataaa
      121 aaacccgcaa tgaatgcggg ttctgactca gtctaagtat tgatgtattt gtgaaacttt
      181 acctttaatg gtgtaaccac cattcagttc gatgggtttg taaagcggat tcagtgacaa
      241 caaatagatg tttggtccgt caatcgcaac tttttttagt gttacgtttg gcgttccttc
      301 caattggatt aagattattt ttcccaccag ttctctaatg ttacttgagc atggtgtgat
      361 cagcacggta gatccgtcgg ggatggttgg gaggccgtta gagtttgtca tcgcatctcc
      421 ctcaacatgc aataaaaaag agttttcagc ggtttttgtc atgacatcaa cccagttatt
      481 aataccagga atcttggtta ctggacaact catatcccaa taaccagcct gttcccacgt
      541 taaaacgggc aaccgggcga tgttgtcact aatgtaaggg tactgattca gacgcagatc
      601 atcggtttta tcgtgacagt cctttccata aagaatccat tcaggagatt tggaaagcaa
      661 ttttgacagt agatacaaat tctcaccgtc aggttttgaa gagccatttt cccattttgt
      721 tacggataca cgagatatgc cgattgcttt cgcaacctgc tgttgggtta atccaacgtc
      781 ttttcgacga ttccgaatac gttcgctgat agtgtttttc atgtaaccaa tgttactacc
      841 aagtgatgtt gctatggttg acattgttat gtaactattg ttacccttct gctcgaaata
      901 acaggagagt tttatgttca aagatgatgt tctgcgctat ttcaaaaaaa agcgactagt
      961 agctgaggct cttggaattt cacatgtggc tgttgtgcgg tggaaagcag ttattcccaa
     1021 acttcgcgca atggaactgg atgaaattac taacagtgaa ttgaaataca acccagaact
     1081 ttacaagaag caggatagca cctcgaacga aggaaagaat gattcatgaa aatcaagcat
     1141 gaacacatcc gcatggcgat gaatgtctgg gcgcatccgg acggcgaaaa agtaccggct
     1201 gcgaaaatta ccaaagcgta tttcgagctg ggaatgacgt tcccggaact gtatgacgac
     1261 agccatccgg aagccctggc ccgtaatacc cagaaaattt tccgttggct ggataaagac
     1321 acccctgatg ctgttgaaaa aatgcaggct ctgttaccgg cgatcgaaaa ggcgatgccg
     1381 cctttgctgg tggcccgtat gcgcagccac agttctgaat attaccgtga gatcgtcgaa
     1441 cggagggatc ggctggtgaa ggatgtcgat gattttgttg cgtcagcggt tgttttgtat
     1501 gaccagatga atcgcggcgg cccggcaggg aatgctgtgg tgatgcacta aaagcacggt
     1561 gttcgggggt tttatgagca gcaagcttca tggtcttgtc tgggaagggt gcgccttcac
     1621 cggcatgatc ttatccaggg tggcggttat ggcccgtctt gcagactaca gcaatgacga
     1681 gggcgtgtca tggcctgcca ttgaaactat ccggcgtcag atcggtgcaa gaagtgaatc
     1741 cacagtgaaa tcggctattg cagaactggc gaaagagggc tggctgacga aggaagagcg
     1801 taaggtcggt gggcgtaatg taagcaatat ctatcggctt aatgtggaaa aactcgaagc
     1861 agctgcggcg gcggcgcgtg agtcatataa accgaaaaga aaaattagcc cggcaaaaaa
     1921 tgacccgtta acagttgacc cgtcaaatat tgacccctca acggttgacc cgtcaaattt
     1981 tgatggatca actgttgata aaaaactgcc gattaggggg gcgatgattg accccgatcc
     2041 gtcagtatta aaacctgatc cgtcagataa aagatcttct tgtccggacg cttcgcaacc
     2101 ggacccgcag acggctgaac aggatttttt aacccgacac cctgacgcgg ttgtgttcag
     2161 tgcgaaaaaa cgccagtggg gaagtcagga agatttggtg tgcgcacagt ggatctgggg
     2221 acgaatcgtg agtctttacg agcaggcggc cagcgatgat ggcgagatca ctagaccgaa
     2281 agaacccaac tggacagcat gggccaatga cgttcgcaca atgcggatgc tggatggcag
     2341 aactcacaga caaatttgtg aaatgtttgg gcgtctccag cgggattcgt tctgggtaaa
     2401 aaacatcatg agtccggcaa aactccggga aaaatgggat gaactggtta tccgcctggg
     2461 gcgttcgcct gcgcagcgtt gcgtgaatca catttctgaa ccggacactg aaataccgcc
     2521 gggattcagg gggtgacgtg tcatgaaaaa cattgcggca gttggggttc ttgaacgtat
     2581 tcgcagactt gcaccacagg ggtcggttcc accgtaccgg acggtggagg agtggcggga
     2641 atggcaactt gctgaaggac gaaaacgcag cgaggagatt aaccgccaga atcgccagtt
     2701 gcgggtggaa aaaatcctga atcgttcggg catccagcct ctgcacagca aatgctcgtt
     2761 tgcaaattat caggtgcaga acgacgggca aaaatacgcg ctgagccagg ccaaatccat
     2821 agctgacgaa ctgatgaccg ggtgcacgaa ttttgtgttc agcggtaaaa ccggcaccag
     2881 gaaaaatcac cttgcagcgg cgatgggcaa ccggctgatg gtgaaggggc gcagcgtgat
     2941 tatcgtcacc gtgtctgacg tcatgagcgt gttgcatgac agctacgaca acggcaaatc
     3001 cggggaaaaa tttttacagg agctttgcgg ggttgatttg ctggtcctgg atgaaatagg
     3061 cgttcagcgg gagacgaaaa acgagcaggt ggtattgcac cagataattg atcgccggac
     3121 agcatcactg tgcagtgtcg ggatgttaac aaacctgaat catgccgcaa tgagtacgct
     3181 tcttggtgag aggattatgg accgcatgac catgaacggt ggtcgatggg tgacgtttaa
     3241 ctgggatagc tggcgtccaa atgtcagcaa tatgagggtt gtgaagtaat tttgtccgga
     3301 ggaaatatta atggaaaccg tatctgacgc actgaaagca ctgaaaagag cctcttcaca
     3361 ggtagtggcg gcccgccttg gaatcagccg tgaagatgcg gtcaacgaac tgtggaaact
     3421 gaagcgccgc ggtgaagcgg ataacaaggg ttcgatgtgg tggctgattc aggctggtga
     3481 aagtgaaccg gtgtcaccgg taccgaaagt gacagcgcaa atgctgactg aggcgattga
     3541 acaacatggc ccacaaacgg cggatgagct ggcactgatg ttcgggatta cctcgcgccg
     3601 ggcgaattca tcgctggcca tggcaatcag caaagggcgt ctgattcgcg tgaatcaggg
     3661 cggtaaattt cgttactgca taccgggcgc tgatttaccg gcagagccgg aagctgcatc
     3721 cgtagcggaa accgatggta aagcctttcc tcagccagca ggtgttgcgt taccagtaca
     3781 ggaaatgatg gcacaggaag aaatgaaaac agaaatcgtg gaagacattg tgaggttaca
     3841 gccatcgatc accgaaatga aagcagatga cctgattcta ccatcgctgc atgtggctaa
     3901 ccgcgagctg cgccgggcga aaagtgatgt ccagaagtgg gagcgagtct gtgctgcgct
     3961 gcgggaactg aacaaacaca gggatattct ccgggatatt accgccacca gagagcagca
     4021 gcggtgagtg gctggaagaa gtggcgctgg gctgaaatcc tgatactccg gcagtgtgcg
     4081 ggaacgatga gagtcgaaag catcggttat ctgattggtc gtagtgagtc agccgtcagg
     4141 acgaaagcgc gggaactggg tatcagcatg attttacggg gtgattttca cccgtcggca
     4201 aaatattcac agcgtgatat tgagctggcg cggcaactgc atcagcgtgg cgtaccccga
     4261 cgggaaattg ccgaaaagtt tgggatgaag ttgggcgcag tgaataacta cgtttatttc
     4321 gacaggaggg ttcaggagtg agggtgagga tttatatcgc cggtccgatg acgggatatg
     4381 aaaatttcaa ccgcgaggcg tttcacaggg cggaagatgc gctgaaacgg gaagggcata
     4441 ccgttttaaa cccggcagta cttccggacg ggctgactca accacactac atggatattt
     4501 gcatggcaat gctccgttgc gtggatgcgg tttacatgct gaaaggctgg cagcagtcgg
     4561 caggtgcaag ggctgagctg gcactggcgg agaaactggg ccatgcggtg atttttcagg
     4621 aggtgggcag tgaatattga cccggcgata acgattgata tggccctgaa caccggcctg
     4681 gcacttcttg gttatttcta catcatgttc tgcagcggac gatggctgtc actgttgttc
     4741 aaaaatggaa taaacgccgt aagcaggagc aacgccagaa ggcaatggat gcatttttcg
     4801 aagccttcgg gattgacagc atggaaccag gggatccagc tcgcgtaatt agcagaggtg
     4861 acgttgtaat tcttgtatac cggaatgaag agaaaaatga gcgagattaa ctatcaggta
     4921 ctgcgtgaaa aggcagagaa agcaactaaa ggaagctaca tcgtagggca tacatctgtt
     4981 aaccaacacg gcaatttaac aggagttttt gtttgtcaaa aatggaaagg agaacccggt
     5041 ggcgtgattg cggaacgtca tgttaactgc ctgattgaaa cagatgctca ggcttatgca
     5101 aacgctgagt tcatagcaga ggctaacccg gctaccgtgc tggcactgct ggatgaacag
     5161 gaaagaaacc agcaatacat caaacgccgc gaccaggaga acgaggatat tgcgcttacg
     5221 gttgggaagc tgctcgttga gcttgaagca gcaaaatcaa aactcaacga gcagcgcgag
     5281 tattacgagg gagttatctc tgatgggtgc aagcgtattg ctgaactgga agcgcgggaa
     5341 gttcaattac cgactcgcta cgaccttcga tatggacacc cgataaatgc agatgagcga
     5401 catgtcatga tacctaaaga aaatggcagt tggctttacc tgattgacct ggaacacgca
     5461 ttacgcgtcg ctggcattcg catcaaagga gaggagcatg gaaataaaac cagaggatga
     5521 gttaagcaat atcgttttat ttccggtaaa agaggatgac cctcgtaatc aggttaattt
     5581 tctttatgag ccatcggaaa gaccatattg tcatcacgcc tctgtccggg ttgacgaaaa
     5641 agagcgtcag gtccgctgta aaatctgcgg tgcagttgtg gaaccatttg actggatgct
     5701 ctctgtggcg aaaagagaaa ccagactggc agatgatgta aggctcttgc gtcaggagga
     5761 gcgggaaagg cgaaaaaata tagaaaagct aattcagatt gagcgtaacg cgaaagcgcg
     5821 gatacgcagg gcgacaaaat ccagaactga ataattaaat ttagctctgt taaaaattta
     5881 atccttaacc ggagggattt ctgcaccctc agaacatcag gaggccgccc gaaagggcgg
     5941 tagttaaatg cgaaagttta aaataattat tgaaacggga atagccggtg gagatttcga
     6001 ggatgaattc gaagtggatg atgatgcgac gcctgatgaa atacatgacg aagcaaaaga
     6061 tattttcttt aactactgca attactcata tcacgaaata aaagacgaag aggaagagca
     6121 aaatggctga ttttggttca actaaataca acgtcagttt tgaagcatgg catgaactgt
     6181 taatggacta tgcagagtta cgtggtggca gtgctgctga tgctgaagca tggcgtgatg
     6241 attatgaagc aggaaaaact ccggtcgaag catattgtga tgagtggggc gatgaatgag
     6301 cgagattaat tatcaggaag ggcatgaaac ggcagggcag gcaaaaccag ttgcatggcg
     6361 atatcgctac gtgaaaaaag gcgttacgga ctttcaggag aatctgtggg ttggtgactg
     6421 gaaatatgta ccgacaaaag aggattgcaa cgacaggccg aactatgaaa ttcaggcctt
     6481 attcgctgcc ccgccagttc cggtgacatc agaagaactg gttaaagctg tgcactttta
     6541 tgaacaacta aaacgcgaaa atccaccagc atccggaaac ctgattacag attcccagat
     6601 aaggcaatga gctacctggc gcagaacggg ctgataagta tggggaatat tttgcgatga
     6661 atatttagac taaagagttt gtaacgctat gtaagtgatt ttttctggtt tagatattta
     6721 tatgtccggc caaattgagg tgtgtttaaa tgttattgca cattgattgt agggggaata
     6781 atgaaaaacg cattgcagtt tttgtttgtt gcgttctggt tgttcgcatc atgtatgccc
     6841 atcatcttca cagcaaggta tatggaaaaa gttgatgttt tgatattaat gtttggacat
     6901 ataaatgccc tttttttagg ggtgttcatg gcggtcatgt gcattgaata ctggcggtaa
     6961 atacagcgaa cgccattggt ttagttggat atttactgtg ccggacaaaa acggtttgcg
     7021 gggaaatctt agttaagtag aatgactgcg ggtgcttgag gctatctgtc tcaggcatga
     7081 acaccaaaag gcagatagag aaaagcccca gttaacatta cgcgtcctgc aagacgctta
     7141 acattaatct gaggccatat ctatgcgaca catagagatt agcctcttac ggaccgaaag
     7201 gtcaaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct gatcgtcatc
     7261 tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc
     7321 cgaaccggcc agacggaggt cgctgtcttc acagcttacg aacctgagga gtaagagacc
     7381 tggcggggga gaaatccctc gccacctctg atgtgtcagg catcctcaac gcacccgcac
     7441 ttaacccgct tcggcgggtt ttgttttttc ctggcattct ggtttacaat tcgcacgtca
     7501 gcctgaacac ctgacacctg ctgcgccagc agagaaaaca gatggcgcac aaaaccaaat
     7561 ttcacaattc tgataccgac cttgccatcc ggcatgagcg gcgttcacac gcatttaaaa
     7621 ccgactggta ccaacaccca ccatgtactg aagaacaggc cgaatggctg attcattctt
     7681 accgcaggcg cgggttcgag gttaagaaag ctctcagtct cgactatcgg cactggataa
     7741 tctctgtcag gctgccttat tccgaacgcc caccacgtcc gtcccgcact ttccagcaac
     7801 ggatctggag gtaacgtgcg ggtattactt agacctgttc tggtgcctga gcttaggctg
     7861 gtggtcctta agccgggccg tgaatccata cagatatttc ataatcctcg agtgctggtg
     7921 gagccggaac cgaaaagcat gtgcggcctg ccatccggag tcgtccctgc cgttcgccag
     7981 ccgctggcgg aggataaatc attactgcca tttttcagca atgagcgtgt gattcgtgct
     8041 gctggcggcg ctggtgcact gtctgactgg ctgttgcgcc atattaaatc ctgccagtgg
     8101 ccacacggtg attatcacca cagtgaaacc gtcattcacc gttatggtac cggcgcaatg
     8161 gtgttgtgct ggcactgcga caaccagttg tgtgaccaga cctcagaatc acttgagcaa
     8221 cttgctcacc aaaacttgtc agcatggatg attgacgtca tccgtcacgc aatcagcggt
     8281 acgcaggaga gggagttatc gctggccgaa ttatcctggt gggcggtctg caatcaggtg
     8341 gctgatgcgc ttccggagtc tgtattgtgt cgttcactgg gattaccggt ggaaaaaatc
     8401 cgctccgtat accgtgagag tgacatcgta ccgggagaac agactgccac cagcatactg
     8461 aagcagcgca caaaaaatat tgcgctgcca cttcacgtcc accagcaaca gcccccactc
     8521 caggaaaaga cgttagtaag catcgccgtt gatccggagt ctccggctca gtatctccag
     8581 cgccagaaac cacaacggga agagatgcct gtatacacgc gctgggtaaa aacgcagaaa
     8641 tgcatgacgt gcggtaatca ggcagatgat ccgcatcaca tcattggtca tggcctggga
     8701 ggtatgggaa caaaggctga tgatttgttt gttattccgc tgtgccgtaa atgtcataac
     8761 gaactgcacg ccggggtaaa agattttgaa gaaaaacacg gcagccagct gttgttgctg
     8821 attcgttttt taatgcacgc gagaaattcg ggtgtcctga agtggaaagc atgaatgact
     8881 gaacgcatag aatttgtttt gccttacccg ccgacggtga atacctactg gcgacgtcat
     8941 ggcaatacgt atttcatctc ggaggccgga aagcgttatc gccgtgatgt ggcgctaatt
     9001 gttcgccagc agcggctgaa attaa
//
LOCUS       DAOTRK010000173         8975 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.174, whole genome shotgun sequence.
ACCESSION   DAOTRK010000173 DAOTRK010000000
VERSION     DAOTRK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.174"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1367)
                     /gene="trkA"
                     /locus_tag="QIE41_004125"
     CDS             complement(<1..1367)
                     /gene="trkA"
                     /locus_tag="QIE41_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDS3532935.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSP"
     gene            complement(1389..2678)
                     /gene="rsmB"
                     /locus_tag="QIE41_004126"
     CDS             complement(1389..2678)
                     /gene="rsmB"
                     /locus_tag="QIE41_004126"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDS3532936.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     VLPGFEEGWVTVQDASAQGCMAWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(2724..3671)
                     /gene="fmt"
                     /locus_tag="QIE41_004127"
     CDS             complement(2724..3671)
                     /gene="fmt"
                     /locus_tag="QIE41_004127"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDS3532937.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKALPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3686..4195)
                     /gene="def"
                     /locus_tag="QIE41_004128"
     CDS             complement(3686..4195)
                     /gene="def"
                     /locus_tag="QIE41_004128"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDS3532938.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4325..5449
                     /gene="dprA"
                     /locus_tag="QIE41_004129"
     CDS             4325..5449
                     /gene="dprA"
                     /locus_tag="QIE41_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDS3532939.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVAGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5421..5894
                     /locus_tag="QIE41_004130"
     CDS             5421..5894
                     /locus_tag="QIE41_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDS3532940.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5923..6465
                     /locus_tag="QIE41_004131"
     CDS             5923..6465
                     /locus_tag="QIE41_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDS3532941.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6470..7042
                     /gene="tsaC"
                     /locus_tag="QIE41_004132"
     CDS             6470..7042
                     /gene="tsaC"
                     /locus_tag="QIE41_004132"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDS3532942.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGATGGRLNPSEIRDALTGELFRQG"
     gene            7047..7865
                     /gene="aroE"
                     /locus_tag="QIE41_004133"
     CDS             7047..7865
                     /gene="aroE"
                     /locus_tag="QIE41_004133"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDS3532943.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGSKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            7862..8119
                     /locus_tag="QIE41_004134"
     CDS             7862..8119
                     /locus_tag="QIE41_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDS3532944.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8095..8649)
                     /locus_tag="QIE41_004135"
     CDS             complement(8095..8649)
                     /locus_tag="QIE41_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDS3532945.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            8678..8914
                     /locus_tag="QIE41_004136"
     CDS             8678..8914
                     /locus_tag="QIE41_004136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3532946.1"
                     /translation="MTLGALLALCKSFSMQKSTVLCANAAKRVKKQQTEKKDQKSTCA
                     KNWDPYNAPPLRRQRETLHKMVVTTKRKNPEIQG"
BASE COUNT         2237 a         2196 c         2235 g         2307 t
ORIGIN      
        1 aaaggacttg gctggaagag tctttcgacg tcggtaataa actttttatc tgtgaggaac
       61 ataattacgt gatcgccttg ctcaatgcgc agattgtcat tggcaatcat cacgtcgttt
      121 ccacgtacca ccgctccaat aatcgttcct ggcggtagct tgatttcatc aatgactctg
      181 ccgacaacgc gtgaggtact ttcatcaccg tgagcaacgg cttcaatagc ttctgctacg
      241 ccgcggcgca atgaggaaac accaacaata tctgcttttc gcacatggct aagcaacgcg
      301 gaaatcgttg cttgttgcgg tgaaatcgca atatcgatca cgctcccctg aaccagatcc
      361 acataagcgc gacgctggat caataccatc acctttttcg cacccatacg tttggcgagc
      421 atggctgaca tgatattggc ctcgtcatcg ttggtgacgg caataaacag atcaacttga
      481 tcgatatgtt cttcggccag cagttcttga tccgacgcat caccaaaaaa gacgatcgta
      541 ttctgcaact tttccgccag ttcggcagcg cgttgctgat tacgttcgat gagtttaacg
      601 ctgtaatctt tttccagacg acgcgccagc cctgcaccga tattaccgcc accaaccagc
      661 atgatccgct tatacggttt ttccagtcgc tgtaattcac tcatcaccgc gcggatatgc
      721 tgtgaagcgg caataaagaa cacttcatca ccagcttcaa caatggtcga accttgcgga
      781 cgaatggggc gatcgtggcg gaaaattgct gccacacgag tatcgatatg tggcatatgt
      841 tcgcgcatgg tcgaaagtgc attaccaatc agcgggccgc cataataggc tttaaccaca
      901 gcgaggctga ctttaccctc agcgaagttc accacctgca atgcgccggg atactcaatc
      961 agtcggtaaa tattatcgat aaccaactgc tctggtgcga tcagatgatc aatcggtaca
     1021 gcatctgaat gaaatagctt atcggcatcg cgcacgtagt ctggtgagcg gatacgagcg
     1081 atgcgattag gggtgttgaa aagtgagtag gctacctggc aggcaaccat attggtttca
     1141 tctgaactag ttacagcaac cagcatatcg gcgtcgtcgg cacctgcctc ccgcaatacg
     1201 cgtggatgag agccatgccc ctgcacgact cgcaggtcaa atttatcctg caaggtccgc
     1261 agacgctcac cgttggtatc gacaacagta atatcgttgt tctcgccaac caggttttcc
     1321 gccagtgtgc cgccaacctg gccggcaccc agaatgataa ttttcatcag tcgcgacccg
     1381 ttatctcatc actttttgat tagcttagcg taaaagaagc cgtcgccctc ttcggcacca
     1441 ggcagatttt gcttacccgg ttgttctggt gttcctgttt cgcaaagttc ggcatcagcg
     1501 gtacgttgca aaaaggcttt aatctgcagg ctattctctt ccggtaacac cgaacaggtg
     1561 gcatagacca gggttccacc ggattttaaa tgcggccaaa tggcgtcgag aatttcagac
     1621 tgcaattgcg cgagttccgg gatatcgcga tcgcgacgta accatttaat atctggatgg
     1681 cgacgaatca caccggttgc tgaacaaggc gcatctaata aaatgcgatc aaactgttgc
     1741 tcgccacacc attgagaagg gtaacggcca tcaccttgtt tcacggtcgc cttcatacca
     1801 aggcgtttta aattgtcgta aacccgagag aggcgctgtt cgtcgatatc aaccgcaaca
     1861 acctgcgctt cgggtgccac ctcaaggata tgcgttgttt taccgccggg ggcagcgcaa
     1921 agatccagaa tgtgttcacc gttttgtggc gcaagccagg ccatgcaacc ttgtgctgat
     1981 gcatcctgaa cggtaaccca tccttcttca aaacctggta gcacatgaac aggtgcaggt
     2041 gtttccagcc gtacagcatc agggtaatcc gcatgcggga aacctttcat tcctgcttca
     2101 tccagcaatg caagccagct gtcgcgggaa tgatgcgtac gattaacgcg cagccacatt
     2161 ggcggacgct ggttattggc ttctacgatg gattgccact gctctggata cgctttttgc
     2221 agacgtttca gcaaccagga aggatgcaga taacgtgcat cactggcatt aaactcggct
     2281 aataactctt cttgctgacg ttggaactgg cgtaatacgc cgttaatcaa ccctttaagt
     2341 tgcggacgct taattgcgac agcaccttca accgtttcag ccagcgcagc atgaggtgga
     2401 atgcgggtat aaagcagttg atacaaacca accataatca ggtaatgcac agtgcgctgt
     2461 ttgccggtca tcggacgggc cattaactta ttaatcagcc agtctaactg tgaaagcgta
     2521 cgcagtacgc caaagcacaa ctcttgaaga agtgctttgt ctttatcgga aactttttgc
     2581 tggagcggtg gcagaatgtt gcttaatgat tgcccttgct cgacgacttg ttcaacggcc
     2641 tgtgccgcca tgctacgtaa attacgttgt tttttcataa gtataaaaat gcccggcaag
     2701 accgggctta gaagagtgga ctatcagacc agacggttgc ccggaacaaa ccattcccga
     2761 cgagaattca ggaggtcttg cgcgctcatc gctttcttac ccgcaggttg taacgagagc
     2821 aggttcagga tgccatcacc agtcgcaacc tgaatgcctt gtttattggc ttcaaggatc
     2881 gttcctggtg cagcgttggt tgccgtatca atgaccgacg ctttccagac tttaaccggc
     2941 tgtccttcaa tttccagcca gctcattggc catggattga aagcgcgaat gcagcgttca
     3001 agctgcgctg ccgaaagcga ccagtcaata cgcgcttctt ctttactcaa cttttcggcg
     3061 taagtgacaa gagtttcgtc ctgaacttct ggtttcgccg tgccgtctgc cagttgtttc
     3121 aatgtggtga taagcccttg tgggccaagc tctgccagct tgtcgtacag agtaccactg
     3181 gtatcttctg cagtaatcgg gcaggagagc ttatagagca tgtcaccggt gtctaaacca
     3241 acatccattt gcataatggt cacaccagtt tctgcatcac ctgcccatag tgagcgttgg
     3301 attggtgcag caccgcgcca gcgtggcagc agtgaaccat gaacgttgat acagccaaga
     3361 cgcggcatct ccagcactgc tttaggcaga attaagccat aggcgacgac aaccataaca
     3421 tcagcctgta gatcggcgac cagttgctgg ttttcttgtg gacgcaggga aaccggttga
     3481 aaaacgggca gagctttttc ctcagccaga actttaaccg ggctgggcat cagtttttta
     3541 ccgcgtcctg ccggtcggtc tggctgggtg aacacgccaa cgacgttatg accagaagac
     3601 aacagcgcgt cgagatgacg cgctgcaaag tcaggtgtac ccgcaaaaat aatacgtagt
     3661 gattctgaca cgttagttct tatccttaag cccgggcttt cagacgatcc agtttttcaa
     3721 ctttctgacg aatacgttgt tgtttcagcg gtgacagata atccataaac agtttgccga
     3781 ccaggtgatc catctcatgc tgaatacaga tggctaacag accctctgct tccagttcaa
     3841 atggtttacc gtcgcggtcc agggcgcgaa ttttaacttt ctctgcgcgt ggcactaaag
     3901 cacgttgttc agggatcgac aggcatcctt cttcaatgcc tgtttcgccg cttttttcta
     3961 aaagctccgg attgattaac accagccgtt cgtcacggtt ttccgaaaca tcaataacaa
     4021 tgatacgttg atggatatcg acctgggttg ccgccaggcc aataccttct tctgcgtaca
     4081 tcgtctcgaa catatcatcg acgatacgct gaatttctgc attcacttct tctaccggtt
     4141 tagcaacttt gcgaagccgc tcgtccggaa tatgtaacac ttgcaaaact gacataaatc
     4201 tccagagatg tgttcaggag ttagaaagat tatttcttct attctagaca aatccccctc
     4261 tgattgacag catcactgac caatcgcaaa gattgctaag gctgcttatg gcagggagat
     4321 aaggatggtc gatacagaaa tttggctgcg tttaatgagc atcagcagct tgtacggcga
     4381 tgatatggtc cgtatagctc actggctggc aaaacagtcg catattgatg cggttgtatt
     4441 gcagcaaact gggcttacat tgcggcaggc acaacgcttt ctttcatttc cgcgaaagag
     4501 tatcgaaagc tcactttctt ggttggagca acccaaccat catttaattc ctgcggacag
     4561 cgaattttat cctcctcaac ttcttgcgac gacagattac cccggcgcac tgtttgttgc
     4621 aggagaactg cacgcgctgc attcatttca gcttgccgta gtggggagtc gggcgcattc
     4681 atggtatggc gagcgatggg gacgattatt ttgcgaaact ctggcgacgc gtggagtgac
     4741 aattacgagt ggactggcgc gtggaatcga tggtgtggcg cataaagcgg ccttacaggt
     4801 aaatggcgtc agcattgctg tattagggaa tggacttaat accattcatc cgcgtcgcca
     4861 tgcccgactg gctaccagtt tgcttgaaca tggtggggct cttgtctcgg aatttcccct
     4921 cgatgttcct ccccttgctt acaatttccc acgaagaaat cgcattatca gtggtctaag
     4981 taaaggtgta ctggtagtgg aagcggcttt gcgcagtggt tcgctggtga cagcacgttg
     5041 tgcgcttgag caggggcgtg aagtttttgc cttgccgggt ccattaggga atccgggaag
     5101 cgaagggcct cactggttaa taaaacaagg tgcgattctt gtgacggaac cggaagaaat
     5161 tctggaaaac ttgcaatttg gattgcactg gttgccagac gcccctgaaa attcatttta
     5221 ttcaccagat caggaagacg tggcattgcc atttcctgag ctcctggcta acgtaggaga
     5281 tgaggtaaca cctgttgacg tcgtcgctga acgtgccggc caacctgtgc cagaggtagt
     5341 aactcaacta ctcgaactgg agttagcagg atggatcgca gctgtacccg gcggctatgt
     5401 ccgattgagg agggcatgcc atgttcgacg tactaatgta tttgtttgaa acctatattc
     5461 acacagaagc tgagttgcgt gtggatcaag acaaacttga acaggatctt accgacgcag
     5521 gatttgatcg agaagatatc tacaatgccc tgctttggct ggaaaagctt gcggattatc
     5581 aggaaggact ggcagaaccg atgcaactgg cctctgatcc actctccatg cgtatttata
     5641 cccctgaaga gtgtgaaaga ctggatgcca gctgtcgtgg ttttttgctt ttccttgagc
     5701 aaattcaggt gcttaacctt gaaacccgtg aaatggtgat agagcgagtg ctcgcgctgg
     5761 ataccgcaga gttcgacctg gaagacctga aatgggtaat cctgatggtg ttgttcaata
     5821 ttccgggctg cgaaaatgcg taccagcaaa tggaagaatt actctttgaa gtgaatgaag
     5881 gtatgctgca ttaatcattt ctttaattca gcataagttg ttatggcgaa atcagcactg
     5941 ttcacggtgc gtaataatga gtcctgccca aagtgcgggg ctgaactggt tattcgatcc
     6001 gggaaacacg gtccgtttct tggatgctca cagtatccgg cgtgtgacta cgtccgtcct
     6061 ctgaaatctt cagcggatgg acatatcgtc aaagttctgg aggggcaggt ttgccctgca
     6121 tgtggcgcaa atctggtatt acgccaggga cgctttggta tgtttattgg ttgcagtaac
     6181 taccctgaat gcgaacatac cgaacttatc gataaaccgg acgaaacagc aattacatgc
     6241 ccccaatgcc ggacgggcca tctggtccag cgccgctccc gttatggcaa aacgtttcac
     6301 tcctgtgatc gctacccgga gtgtcaattt gccattaact tcaaacccat agctggagaa
     6361 tgccctgagt gtcattatcc gctactcatc gaaaagaaaa ccgcgcaggg tgtaaaacac
     6421 ttttgtgcca gtaaacaatg tggaaagccg gtttcggcgg aataataacg tgaataataa
     6481 cctgcaagga gacgctatcg cagctgcgat agatgtcctc aatgaagaac gtgtcatcgc
     6541 ctatccaacg gaagccgttt tcggtgtcgg gtgcgatcct gatagcgaaa cagcagtgat
     6601 gcgactgttg gagttaaaac agcgtccggt tgataagggg ctgattctaa tcgccgcaaa
     6661 ttacgagcag cttaaaccct atattgatga caccatgctg actgacgcgc agcgtgaaac
     6721 tatattttct cgctggccag gtcctgtcac ctttgttttt cccgcgcctg cgacaacacc
     6781 gcgctggttg acgggccgct ttgattcgct tgctgtacga gtcaccgacc atccgttggt
     6841 ggttgctttg tgccaggctt atggtaaacc gctggtttct accagtgcca acttgagtgg
     6901 attgccacct tgtcgaacag tagacgaagt tcgcgcacaa tttggcgcgg cgttcccggt
     6961 tgtgcctggt gcaacggggg ggcgtttaaa tccttcagaa atccgcgatg ccctgacggg
     7021 tgaactgttt cgacaggggt aacataatgg aaacctatgc tgtttttggt aatccgatag
     7081 cccacagcaa atcgccattc attcatcagc aatttgctca gcaactgaat attgaacatc
     7141 cctatgggcg cgtgttggca cccatcaatg atttcatcaa cacactgaac gctttctttc
     7201 gtgctggtag taaaggtgcg aatgtgacgg taccttttaa agaagaggct tttgccagag
     7261 cggatgagct tactgaacgg gcagcgttgg ctggtgctgt taataccctc aagcggttag
     7321 aagatggacg cctgctgggt gacaataccg atggtatagg tttgttaagc gatctggaac
     7381 gtctgtcttt tatccgtcct ggtttacgta ttctgctcat tggcgctggt ggagcatctc
     7441 gcggcgtact actgccactc ctttccctgg actgtgcggt gacaatcact aatcggacgg
     7501 tatctcgcgc agaagagttg actaaattgt ttgcgcacac tggcagtatt caggcgttgg
     7561 gtatggacga actggaaggt catgagtttg atctcattat taatgcaaca tccagtggca
     7621 tcagtggtga tattccggcg atcccgtcat cgctcattca tccaggcatt tattgctatg
     7681 acatgttcta tcagaaagga aaaactcctt ttctggcatg gtgtgagcag cgaggctcaa
     7741 agcgtaatgc tgatggttta ggaatgctgg tggcacaggc ggctcatgcc ttccttcttt
     7801 ggcatggcgt tctgcctgat gtagaaccgg tcatcaaact attgcaacag gagttgtcag
     7861 cgtgaatcag gccatccagt ttccggatag ggaagagtgg gacgagaata aaagatgtgt
     7921 atgttttccc gctctcgtga atggtatgca actgacatgt gcgatctctg gcgagagtct
     7981 ggcgtatcgc tttactggag atacgccaga acagtggtta gcgagttttc gtcagcatcg
     8041 ctgggacctg gaagaagaag cggaaaactt aattcaggaa caaagtgaag atgatcaagg
     8101 ttgggtctgg ttaccttgat ccagatattc gtccttccat ttcacgtagt tatttgcgga
     8161 atagcgtaac ccagccttct cttcatcact taacgggcgg atctgtttga cggggctacc
     8221 gagatacaga tatccgctct ccagccgttt attttgtggg accaggctac ccgcaccaat
     8281 catcacatca tcttctacta ttgcgccatc aagtaaaatt gagcccatcc caaccaaaac
     8341 tcgattgcca atggtgcagc cgtggagcat caccttgtga ccaacagtga catcttcgcc
     8401 aatggttaat gggttgccat ctgggttgta cgaggattta tgagtgacat gcaacatact
     8461 gccatcctgg atattggtgc gtgctccgat ctgtacataa tgtacatctc cacgaatcac
     8521 aacgagcggc cagatcccca catcatcagc cagacgaacg tcaccaatca cgacgctgct
     8581 atcgtcgatc attacgcgct gaccgatttg tggaaaaaga tcgcggtatg ggcgtaaaac
     8641 atcagacata ctcacctcaa caataaatga tttcctaatg actttgggtg cattattggc
     8701 cttgtgcaag tcttttagta tgcaaaaaag caccgttttg tgtgcgaatg cagcaaaaag
     8761 ggtgaaaaaa caacaaacag aaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga
     8821 tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacaaga tggtcgtaac
     8881 aacaaagaga aaaaatcctg aaattcaggg ttgactctga aagaggaaag cgtaatatac
     8941 gccacctcgc gacagtgagc tgaaagctgc gtcgc
//
LOCUS       DAOTRK010000174         8876 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.175, whole genome shotgun sequence.
ACCESSION   DAOTRK010000174 DAOTRK010000000
VERSION     DAOTRK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.175"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            167..2545
                     /gene="ppsA"
                     /locus_tag="QIE41_004137"
     CDS             167..2545
                     /gene="ppsA"
                     /locus_tag="QIE41_004137"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDS3532947.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRSDMGLTNVEIMIPFVRTVDQAKAVVDELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2602..4248)
                     /gene="fadK"
                     /locus_tag="QIE41_004138"
     CDS             complement(2602..4248)
                     /gene="fadK"
                     /locus_tag="QIE41_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDS3532948.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQLVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCCMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4305..4598)
                     /locus_tag="QIE41_004139"
     CDS             complement(4305..4598)
                     /locus_tag="QIE41_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDS3532949.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4595..5884)
                     /locus_tag="QIE41_004140"
     CDS             complement(4595..5884)
                     /locus_tag="QIE41_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS3532950.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDVLEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRIVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5940..6878)
                     /locus_tag="QIE41_004141"
     CDS             complement(5940..6878)
                     /locus_tag="QIE41_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDS3532951.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTAH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6898..7662)
                     /locus_tag="QIE41_004142"
     CDS             complement(6898..7662)
                     /locus_tag="QIE41_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDS3532952.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQIATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGCGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPINQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEYLKKALN"
     gene            7978..>8876
                     /locus_tag="QIE41_004143"
     CDS             7978..>8876
                     /locus_tag="QIE41_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDS3532953.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPV"
BASE COUNT         2161 a         2271 c         2269 g         2175 t
ORIGIN      
        1 attgtcgaag agagcagatt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
       61 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
      121 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
      181 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
      241 gggcaaaaat gcctccctgg gtgaaatgat tactaacctt tccggaatgg gtgtttccgt
      301 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
      361 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta cccagcttgc
      421 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
      481 aaacgccatc cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcgtttgc
      541 ggtgcgttcc tccgctactg cagaagatat gccggacgct tcttttgccg gtcagcagga
      601 aactttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
      661 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
      721 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
      781 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
      841 gggccttggt gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcacaa
      901 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
      961 ccgcatggtc tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
     1021 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
     1081 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
     1141 ccacaccggc aaactgttca ttgtgcaggc gcgcccggaa accgtgcgct cacgcggtca
     1201 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
     1261 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
     1321 catcgaacct ggcgacgtgc tggttactga catgactgac ccggactggg aaccgatcat
     1381 gaagaaggca tctgccatcg tcaccaatcg tggcggtcgt acctgtcacg cggcgatcat
     1441 cgctcgtgaa ctcggcattc cggcggtagt gggctgtggt gatgcaacag aacggatgaa
     1501 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttatg tctatgcgga
     1561 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgcctgatc tgccgttgaa
     1621 ggtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgaacga
     1681 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
     1741 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
     1801 gaaaggtttt gattctccgc gtgaatttta cgttgggcgt ctgactgaag ggatcgcgac
     1861 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
     1921 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
     1981 cggcttccgt ggcgcgggcc gctatgtttc cgacagcttc cgcgactgct ttgcgctgga
     2041 gtgtgaagca gtgaaacgtg tgcgcagcga catgggactg accaacgttg agatcatgat
     2101 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gacgaattgg cgcgtcaggg
     2161 gctgaaacgt ggtgagaacg ggctgaaaat catcatgatg tgtgaaattc cgtccaacgc
     2221 cttgctggca gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgacat
     2281 gacacagctg gcgctcggtc tggaccgtga ctccggagtg gtgtctgaac tgttcgatga
     2341 gcgcaacgat gcggtgaaag cactgctgtc gatggcgatt cgtgccgcga agaaacaggg
     2401 caaatatgtc gggatttgcg gtcagggccc gtccgaccac gaagactttg ccgcatggtt
     2461 gatggaagaa gggatcgata gcctgtctct gaacccggat accgtggtgc aaacctggtt
     2521 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccgggttta
     2581 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgag ttaatctccg
     2641 cataatatct ttccttaaca aaaacttttg tattttacct gaggcagtgc gcggtagttt
     2701 ttcgattacc acgatatgtt caggatattt atatttcgcg acccgtttac ggctaaaaaa
     2761 agccaccacc tcttccagcg ataatgaatg atgcggtgct ttcagcacga cataagcgca
     2821 tgagcgttca cctaaacgtt catcgggcat cgcaaccaca caggcatcgt gaattttagg
     2881 atgctgcaat aaaatatctt ctacttcacg gctgctaata ttttcgccgc cgcggacaat
     2941 aatatctttc ttgcgcccgg ttatttttat atagccggcc tcatccatgc agcagagatc
     3001 gccgctgtaa taccagcctt cttcatccag ggcacgggcg gttaattcag gttcatcaaa
     3061 ataccccata aacacattgg ggccacgcga ggcttcttca ccttcgcaac ctggcggtaa
     3121 ggtcttgcgt gcgtcatcga ccactttaat ctcaacacct gcggcagcgt aaccatcggt
     3181 gtgcataaag cgcgacaaag gatcgtcgag attcaccact gcgtgcggcg aactttctgt
     3241 ggaaccataa acacttaata atttaatgcc gcgctgctgg cattcacgcg ccactttttt
     3301 ggggattgtg gtcccgccgc aaagaaagaa gcgcagcgct gaaaggtcag cgggttgttt
     3361 ctccagtaaa ttcaaaagat catagacaaa cggcgttgcg ccgagcatac aggtgcaacg
     3421 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
     3481 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcat gaccaagtgg
     3541 cgcaggcatc ataaagacat cctgccaggt cagattcaac cgcgcgcaat aagcccgctc
     3601 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
     3661 ggtaaacagc accgcggcta attcatcgcc gtggaccgtt atcgccgtgg ttagaggagt
     3721 attgtcggcg ataatctgac ttaatgagag agcagaggtg gcgggagcca gtttgtccac
     3781 gccgacaagt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
     3841 acgcgtttgt ttaaacaacg tcggagcaaa gaacattttt gcctgacact tattgagcac
     3901 ccatactagt tctgcttccc gccaggaagg caacagcggt acggaaaccg caccgatttt
     3961 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
     4021 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
     4081 gagcgcgcta taggtgtacg atgctccatg attatcgacc acggcaattt tgtctggcat
     4141 cgcacgagcg gtctgctgcc agtaatcggc cagcgaagca tcgccccata gcccttgctg
     4201 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
     4261 agacagaaca tcaggcccga gatgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
     4321 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt gccgcacagc
     4381 acccgacagg tgccgcactc cagacaaccg gcagaatcaa aatgaatgtt gccggcgtca
     4441 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
     4501 ggatttgccg ccaaaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
     4561 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
     4621 catctttcag caagttgatc agcccaattt ttttcgcgtg tcccatgatc attttgcgta
     4681 ccggctggtt tggtttgccg tcaatggtga acatatcgtt catgatgtcg gcgacaatgc
     4741 gcggatactg actaaacagg cgcggatttt ccatcagcgc cgggatcttg cggaaatgct
     4801 gcatatcgcg cataacacaa ctttgttcca gctcacgttt gtattgcgcc agactgctgg
     4861 cggagaaatc cgcgcgttct ttggcggcaa tcactgttgt ggcggcagcc tgagctgatg
     4921 caatggctaa atccatgccg cgaactgtaa aacccaaatt caggcagaag cctgcggcgt
     4981 caccaacgat catcacgcca tcgttaacca gctgcggcac cattgccaga ccgccttccg
     5041 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
     5101 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
     5161 ccaatccaca aaccagcccc aacgatatgg aatccttatt ggtatagagg aatccgccac
     5221 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
     5281 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
     5341 cgtaatgatg cggatcggaa gcggaaacca ttcccagcga gcggccaagc atcgagttaa
     5401 cgccatcagc cagaatcacc acattcgctt caagaacatc atccccggcc tgcacgccag
     5461 tgaccttgtt tccttcacga accagcgcat cgacgcgtac gcccgggata aactgcgcgc
     5521 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
     5581 acgcgtgttg cggaacatct ggttgctcgc ggtgaaagtc gagggtaacg gcgctttctt
     5641 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatgctgcaa
     5701 agcctggaat aatcgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
     5761 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgc gccatgacca
     5821 gtgcggcaac gctaccggca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
     5881 acatgcgtta tgctcccttc ccgctgcgtt tacagcggga gatgtggttg aaaggggaat
     5941 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
     6001 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
     6061 gcgccgttgc cgccaaccat atgctggatc tgcccggaga tccccagcgt caggtagaga
     6121 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
     6181 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
     6241 accattttca ggtcatcctg cgctgccaga ccacgaccga cgccaaccac acgttttgct
     6301 ttgctcaggt ccacgctgct tgccgctttc gcacggcgtt cgcgacagag aatttcatga
     6361 cgcggggcaa cataaggtac cgtttctgtc gggcactgat gagcggtatc actggtgcac
     6421 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctgt
     6481 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
     6541 accgcagtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
     6601 gctttaccgc gtttcgtcgc ggccagcagc aacatagaag gatgtttatc tttcagcagg
     6661 gcagcaatgc tttcggcgta attttcagta cgttgcagcg catcattttg ctcaagaaca
     6721 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
     6781 gcatacacct gttggcccca ttgctgcgcg ccgccaaaca gttcagcata acgttcagga
     6841 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
     6901 gttcagggct ttcttcagat attccgccag ctcggcaatg gcctccggcg aatcgttatc
     6961 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccggtca gctcagcaag
     7021 tggcgcgctc tggctccagc caatgtcact tgcctgccac tgatttatcg gttttttacc
     7081 cgcgccgaga atggctttca tcgaaggaat gcgtggcatg ttaatatcgg aggtgacgca
     7141 gagcacggct ggaacagaga gctcaataac ttcaacatca tcttcaagcg tgcgttcaat
     7201 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
     7261 aatttctccg accagcaagc caacctgctg ggcataaagg tcaccgcaac cttcaccaaa
     7321 gatcagtaaa tcgaagccga tcttttcaac tgctgccgcc agtgcttttg cggtatcgag
     7381 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctatgcg gcccgcggga
     7441 tagcacgtct ttgcgtactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
     7501 tatctcatcg tcatctgttg caatctggct tgcagcttca atggcattga gatcgaactg
     7561 gctgattttg gcgtcggcat tgtcgaaatt cagggtatat tctggagtga caacaatgtc
     7621 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
     7681 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
     7741 gcggggtttt cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga
     7801 tcgctaaaac aaaagtgcaa tttgcggcaa tatagttatt ccatgaccgt acaatcttgc
     7861 attcagtgta aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac
     7921 gccgtatttt atccggcata tattgttttt gcttgctcat ggaataggga ggttgtcatg
     7981 tatcaacgct gttttgataa cgccagcgaa acgctgtttg tcgccggtaa aacgccacgg
     8041 ctttcacgtt ttgcatttag cgatgatcca aaatgggagt ccggacatca cgttcatgac
     8101 aatgaaaccg agttgattta cgtcaagaaa ggggtagcaa ggtttaccat cgattcttcg
     8161 ttatatgtcg cgcacgccga tgacattgtg gtgatagaac gtggcaggct gcatgcggtg
     8221 gcctctgacg ttaacgatcc ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt
     8281 cagggggctg aggaaaatca gctactgcaa ccgcattctt gcccggtaat tgccgcaggg
     8341 cagggaaaag aagttattaa aaccttattt aatgagctaa gtgtgatttt gccgcaaagt
     8401 aaaaatagcc aaacatcttc gttatgggac gcatttgcct atacgttagc aattctttac
     8461 tatgaaaact ttaaaaatgc ttatcgttcg gagcagggat atattaaaaa agatgttctg
     8521 ataaaagata ttcttttcta tctgaataat aactatcgcg aaaaaatcac tttagagcag
     8581 ttatcgaaaa agtttcgtgc cagcgtcagt tatatttgcc atgaatttac caaagagtat
     8641 cgtatttccc ctattaacta tgttattcaa cggcgtatga cggaagcgaa atggtcactc
     8701 actaatactg aattatcaca ggcagagatc tcctggcgtg tgggttatga aaatgtcgat
     8761 cactttgcca aactgttttt gcgccatgtc ggctgttcgc ccagcgatta ccgcaggcaa
     8821 tttaaaaact gttttgcgga acaagaaatc ttatctgaat ttcctcaacc ggtaag
//
LOCUS       DAOTRK010000175         8673 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.176, whole genome shotgun sequence.
ACCESSION   DAOTRK010000175 DAOTRK010000000
VERSION     DAOTRK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.176"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(254..517)
                     /gene="rpsT"
                     /locus_tag="QIE41_004144"
     CDS             complement(254..517)
                     /gene="rpsT"
                     /locus_tag="QIE41_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDS3532954.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            620..838
                     /locus_tag="QIE41_004145"
     CDS             620..838
                     /locus_tag="QIE41_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDS3532955.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            846..1787
                     /gene="ribF"
                     /locus_tag="QIE41_004146"
     CDS             846..1787
                     /gene="ribF"
                     /locus_tag="QIE41_004146"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDS3532956.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1830..4646
                     /gene="ileS"
                     /locus_tag="QIE41_004147"
     CDS             1830..4646
                     /gene="ileS"
                     /locus_tag="QIE41_004147"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDS3532957.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKELLGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLFAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4646..5140
                     /gene="lspA"
                     /locus_tag="QIE41_004148"
     CDS             4646..5140
                     /gene="lspA"
                     /locus_tag="QIE41_004148"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDS3532958.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5228..5677
                     /gene="fkpB"
                     /locus_tag="QIE41_004149"
     CDS             5228..5677
                     /gene="fkpB"
                     /locus_tag="QIE41_004149"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDS3532959.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5679..6629
                     /gene="ispH"
                     /locus_tag="QIE41_004150"
                     /gene_synonym="lytB"
     CDS             5679..6629
                     /gene="ispH"
                     /locus_tag="QIE41_004150"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDS3532960.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6695..7609
                     /gene="rihC"
                     /locus_tag="QIE41_004151"
     CDS             6695..7609
                     /gene="rihC"
                     /locus_tag="QIE41_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDS3532961.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFLSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7776..8597
                     /gene="dapB"
                     /locus_tag="QIE41_004152"
     CDS             7776..8597
                     /gene="dapB"
                     /locus_tag="QIE41_004152"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDS3532962.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         1979 a         2167 c         2467 g         2060 t
ORIGIN      
        1 atcacaatct ccacattctg tgtaggggga gtctgaagat tgagtagata ttcttattca
       61 gaatatatca gccgatgact ctacgattct taagtcacga agagtacaga gagtgtaact
      121 gcatgtctgc aagaacctgt aacttttgac tatctggcaa ccggcagtgt gttttctcac
      181 gcatcacaaa agcagcaggc ataaaaaaac ccgcttgcgc gggctttttc acaaagcttc
      241 agcaaattgg cgattaagcc agtttgttga tctgtgcagt caggttagcc ttatgacgtg
      301 cagctttgtt tttgtggatc agacctttag cagcctgacg gtccacgatc ggttgcattt
      361 cgttaaatgc tttctgtgca gcagctttgt cgccagcttc gatagctgcg tatactttct
      421 tgatgaaagt acgcatcata gagcgacggc ttgcgttgtg cttacgagcc ttttcagact
      481 gaatggcgcg cttcttagct gatttgatat tagccaaggt ccaactccca aatgtgttct
      541 atatggacaa ttcaaaggcc gaggaatatg cccttttagc cttcttttgt caatggattt
      601 gtgcaaataa gcgccgttaa tgtgccggca ctcgttacgt agtgatggcg caggattcta
      661 ccagcttgcg gggtgtgaat acagcttttc cgcgataaaa attgcagcag gcggtcagtt
      721 tcttcccgtg atttgcgcca tggcaatgaa aagccacttc tttctgattt cggtactcaa
      781 tcgccggtta accttgaccg ctgtacaagg tatactcgga cgattttcac tgttttgagc
      841 cagacatgaa gctgatacgc ggcatacata atctcagcca ggccccgcaa gaagggtgtg
      901 tgctgactat tggtaatttc gacggcgtgc atcgcggtca tcgcgcgctg ttacagggct
      961 tgcaggaaga agggcgcaag cgcaacttac cggtgatggt gatgcttttt gaacctcaac
     1021 cactggaact gtttgcaacc gataaagccc cggcacggct gacccggctg cgggaaaaac
     1081 tgcgttacct tgcagagtgt ggcgttgatt acgtgctgtg cgtacgtttc gacaggcgtt
     1141 tcgcggcgtt aaccgcgcaa aatttcatca gcgatcttct ggtgaagcat ttgcgcgtaa
     1201 aatttcttgc cgtaggtgat gatttccgct ttggcgctgg tcgtgaaggc gatttcttgt
     1261 tattacagaa agctggcatg gaatacggct tcgatatcac cagtacgcaa actttttgcg
     1321 aaggtggggt gcgtatcagc agcaccgccg tgcgtcaggc ccttgcggat gacaatctgg
     1381 ctctggcaga aagtttactg gggcacccgt ttgccatctc cgggcgtgta gtccacggtg
     1441 atgaattagg gcgcactata ggtttcccga cggcgaatgt accgctgcgc cgtcaggttt
     1501 ccccggtgaa aggggtttat gcggtagaag tgctgggcct cggtgaaaag ccgttacccg
     1561 gcgtggcaaa catcggaaca cgcccaacgg ttgccggtat tcgccagcag ctggaagtgc
     1621 atttgttaga tgttgcaatg gacctttacg gtcgccatat acaagtagtg ctgcgtaaaa
     1681 aaatacgcaa tgagcagcga tttgcgtcgc tggacgaact gaaagcgcag attgcgcgtg
     1741 atgaattaac cgcccgcgaa ttttttgggc taacaaaacc ggcttaagcc tgttatgtaa
     1801 tcaaaccgaa acacggaacc gagaatctga tgagtgacta taaatcaacc ctgaatttgc
     1861 cggaaacagg gttcccgatg cgtggcgatc tcgccaagcg cgaaccggga atgctggcgc
     1921 gttggactga tgatgatctg tacggcatca tccgtgcggc taaaaaaggc aaaaaaacct
     1981 tcattctgca tgatggccct ccttatgcga atggcagcat tcatattggt cactcggtta
     2041 acaagattct gaaagacatt atcgtgaagt ccaaagggct ttccggttat gactcgccgt
     2101 atgtgcctgg ctgggactgt catggtctgc caatcgaact gaaagtagag caagaatacg
     2161 gtaagccggg ggagaaattc accgccgctg agttccgcgc caagtgccgc gaatacgctg
     2221 cgacccaggt tgacggtcag cgcaaagact ttatccgtct gggcgtgctg ggtgactggt
     2281 cccacccgta cctgaccatg gacttcaaaa ctgaagccaa tatcatccgc gcgctgggca
     2341 aaatcatcgg caacggtcac ctgcacaaag gcgcgaagcc ggtacactgg tgcgttgact
     2401 gccgttctgc gctggcggaa gcggaagttg agtattacga caaaacttct ccgtccatcg
     2461 acgttgcttt ccaggcggtc gatcaggatg cgctgaaagc aaaatttgcc gtaagcaacg
     2521 ttaacggccc aatctcgctg gtgatctgga ccactacgcc gtggactctg cctgcgaacc
     2581 gcgcaatctc tattgcacct gatttcgact atgcgctggt gcagatcgac ggtcaggccg
     2641 tgattctggc gaaagatctg gttgaaagcg taatgcagcg tatcggcgtg accgattaca
     2701 ccattctcgg cacggtaaaa ggtgcggagc ttgagctgct gcgctttacc catccgttta
     2761 tgggcttcga cgttccggca atcctcggcg atcacgttac cctggatgcc ggtaccggtg
     2821 ccgttcacac cgcgcctggc cacggcccgg acgactatgt gatcggtcag aaatacggcc
     2881 tggaaaccgc taacccggtt ggcccggacg gcacttatct gccgggtact tacccgacgc
     2941 tggatggcgt gaacgttttc aaagcgaacg acatcgttgt tgcgctgctg caggaaaaag
     3001 gcgcgctgtt gcacgttgag aaaatgcagc acagctatcc gtgctgctgg cgtcacaaaa
     3061 cgccgatcat cttccgcgcg acgccgcagt ggttcgtcag catggatcag aaaggtctgc
     3121 gtgcgcagtc actgaaagag atcaaaggcg tgcagtggat cccggactgg ggccaggcgc
     3181 gtatcgagtc gatggttgct aaccgtcctg actggtgtat ctcccgtcag cgcacctggg
     3241 gcgtaccgat gtcactgttc gtgcataaag acacggaaga gctgcatccg cgtaccctcg
     3301 aactgatgga agaagtggct aaacgcgttg aagttgacgg catccaggcg tggtgggatc
     3361 tcgatgcgaa agagctcctc ggcgacgaag ctgatcagta cgtgaaagtg ccggatacgc
     3421 tggatgtatg gtttgactcc ggatctaccc actcttccgt tgttgacgtg cgtccggaat
     3481 ttgccggtca cgctgcggac atgtatctgg aaggttcaga ccagcaccgt ggctggttca
     3541 tgtcctctct gatgatctct accgcgatga agggcaaagc accgtatcgt caggtactga
     3601 cccacggctt taccgtggat ggtcagggcc gcaagatgtc taaatccatc ggcaacaccg
     3661 tttcgccgca ggatgtgatg aacaaactgg gcgcggatat tctgcgtctg tgggtggcat
     3721 caactgacta caccggtgaa atggccgttt ctgacgagat cctgaaacgt gctgccgaca
     3781 gctatcgtcg tatccgtaac accgcgcgct tcctgctggc aaacctgaac ggttttgatc
     3841 cggcaaaaga tatggtgaaa ccggaagaga tggtggtact ggatcgctgg gccgtaggtt
     3901 gtgcgaaagc ggcacaggaa gacatcctca aggcgtacga agcatacgat ttccacgaag
     3961 tggtacagcg tctgatgcgc ttctgctccg ttgagatggg ttccttctac ctcgacatca
     4021 tcaaagaccg tcagtacacc gccaaagcgg acagtgtggc gcgtcgtagc tgccagacag
     4081 cgctgtatca catcgcggaa gcgctggtgc gctggatggc accaatcctc tccttcaccg
     4141 ctgatgaagt gtggggctac ctgccgggtg aacgtgaaaa atacgtcttc accggcgagt
     4201 ggtacgaagg cctgtttggt ctggcagaca gtgaagcgat gaacgatgcg ttctgggacg
     4261 agctgttgaa agtgcgtggc gaagtgaaca aagtcattga gcaagcgcgt gccgacaaga
     4321 aagtgggtgg ctcgctggaa gcggcagtga ccttgttcgc agaaccggaa ctggcggcga
     4381 aactgaccgc gctgggcgat gaattacgat ttgtcctgtt gacctccggc gctaccgttg
     4441 cagactataa cgacgcacct gctgatgccc agcagagcga agtactcaaa gggctgaaag
     4501 tcgcgttgag taaagccgaa ggtgagaagt gcccacgctg ctggcactac acccaggatg
     4561 tcggcaaggt agcggaacac gcagaaatct gcggccgctg tgtcagcaac gtcgccggtg
     4621 acggtgaaaa acgtaagttt gcctgatgag tcaatcgatc tgttcaacag ggctacgctg
     4681 gctgtggctg gtggtagtcg tgctgattat cgatctgggc agcaagtacc tgatcctcca
     4741 gaactttgct ctgggggata cggtcccgct gttcccgtcg cttaatctgc attatgcgcg
     4801 taactatggc gcggcgttta gtttccttgc cgatagcggc ggctggcagc gttggttctt
     4861 tgccggtatt gcgattggta ttagcgtgat cctggcagtg atgatgtatc gctcgaaggc
     4921 cacgcagaag ctaaacaata tcgcttacgc gctgattatt ggcggcgcgc tgggcaacct
     4981 gttcgaccgc ctgtggcacg gcttcgttgt cgatatgatc gacttctacg tcggcgactg
     5041 gcacttcgcc accttcaacc ttgccgatac tgccatttgt gtcggtgcgg cactgatcgt
     5101 gctggaaggg tttttacctt ctaaagcgaa aaaacaataa taaaccctgc cggatgcggc
     5161 ctacagattg ctacgaaatc gtaggcagca tccggcaaaa atcctcaaaa tataagagca
     5221 aacctgcatg tctgaatctg tacagagcaa tagcgccgtc ctggtgcatt tcacgctaaa
     5281 actcgacgat ggcaccaccg ccgagtctac ccgcaacaac ggtaaaccgg cgctattccg
     5341 cctgggcgat gcttctcttt ccgaagggct ggagcaacac ctgctggggc tgaaagtggg
     5401 cgataaaacc actttctcgc tggagccaga tgcggcgttt ggcgtgccgt caccggatct
     5461 gattcagtac ttctcccgcc gtgagtttat ggatgcaggc gagccagaaa ttggcgcaat
     5521 catgcttttt accgcaatgg atggcagtga gatgcctggc gtgatccgcg aaattaacgg
     5581 cgactccatt accgttgatt tcaaccatcc gctggccggg cagaccgttc attttgatat
     5641 tgaagtgctg gaaatcgatc cggcactgga ggcgtaacat gcagatcctg ttggccaacc
     5701 cgcgtggttt ttgtgccggg gtagaccgcg ctatcagcat tgttgaaaac gcgctggcca
     5761 tttacggcgc accgatatat gtccgtcacg aagtggtgca taaccgctac gtggtcgata
     5821 gcttgcgtga gcgtggggct atctttattg agcagattag cgaagtgccg gacggcgcaa
     5881 tcctgatttt ctccgcacac ggtgtttctc aggccgtacg taacgaagca aaaagccgcg
     5941 atttgacggt gtttgatgcc acctgtccgc tggtgaccaa agtgcatatg gaagtcgccc
     6001 gcgccagccg tcgtggcgaa gaatctattc ttatcggtca cgccgggcac ccggaagtgg
     6061 aagggacgat gggtcagtac agcaacccgg aagggggaat gtatctggtc gaatcgccag
     6121 acgatgtgtg gaaactgacg gtcaaaaacg aagagaagct ctcctttatg acccaaacca
     6181 cgctgtcggt ggatgacacg tctgatgtga tcgacgcgct gcgtaaacgc ttcccgaaaa
     6241 ttgtcggtcc gcgcaaagat gacatctgct acgccactac taaccgtcag gaagcagtac
     6301 gcgccctggc agagcaggcg gaagtggtgc tggtggtcgg ttcgaaaaac tcctctaact
     6361 ccaaccgtct ggcggagctg gctcagcgga tgggcaaacg cgcgtttttg attgacgatg
     6421 cgaaagatat ccaggaagag tgggtgaaag aggttaaatg cgtcggcgtg actgcgggcg
     6481 catcggctcc ggatattctg gtgcagaatg tggtggcacg tttgcagcag ctgggcggtg
     6541 gtgaagccat tccgctggaa ggccgtgaag aaaacattgt tttcgaagtg ccgaaagagc
     6601 tgcgtgtcga tattcgtgaa gtcgattaag tcattagcag cctaagttat gcgaaaatgc
     6661 cggtcttgtt accggcattt tttatggaga aaacatgcgt ttacctatct tcctcgatac
     6721 tgaccccggc attgacgatg ccgtcgccat tgctgccgcg atttttgccc ccgaactcga
     6781 cctgcaactg atgaccaccg tcgcgggtaa tgtctcggtt gagaaaacca cccgcaacgc
     6841 cctgcaactg ctgcatttct ggaatgcgga gattccgctc gctcagggag ccgctgtgcc
     6901 gctggtacgc gcaccgcgtg atgcggcatc tgtgcacggc gaatcgggaa tggctggcta
     6961 cgactttgtt gagcacaacc gaaagccact tgggatacca gcgtttctgg cgattcgtga
     7021 tgccctgatg cgtgcgcctg agcctgttac cctggtggcc atcggcccgt taaccaatat
     7081 tgcgctgtta ctttcacaat gcccggaatg taaaccgtat attcgtcgtc tggtgatcat
     7141 gggcggttct gctggtcgcg gcaactgcac accaaacgcc gagtttaata tcgctgccga
     7201 tccagaagcg gcagcctgtg tcttccttag cggcatcgaa atcgtcatgt gcggtctgga
     7261 tgtcaccaat caggcaatat taacccctga ctatctcgct actttgccgg agttgaatcg
     7321 caccggaaaa atgctccacg ccctgtttag tcactaccgt agcggcagta tgcaaagtgg
     7381 actgcgaatg cacgatctct gcgccatcgc ctggctggtg cgcccggacc tgttcactct
     7441 caagccgtgt tttgtggcag tggaaacgca gggcgaattt acctctggca cgacggtggt
     7501 tgatatcgac ggttgcctgg gcaaaccagc caatgtacag gtggcattgg atctgaatgt
     7561 aaaaggcttc cagcagtggg tagctgaggt gctggctctg gtgccgtaac ctgtcacatg
     7621 ttatcggcat gcagtcattc atcgactcat gcctttcact gatatccctc cctgtttatc
     7681 attaatttct aattatcagc gtttttggct ggcggcgtag cgatgcgctg gttactctga
     7741 aaacggtcta tgcaaattaa caaaagagaa tagctatgca tgatgcaaac atccgcgttg
     7801 ccatcgcggg agccgggggg cgtatgggcc gccagttgat tcaggcggcg ctggcattag
     7861 aaggcgtgca gctgggcgcg gcgctggagc gcgaaggatc ttctttactg ggcagcgacg
     7921 ccggtgagct ggctggagca gggaaaacag gcgttaccgt gcaaagcagc ctcgatgcgg
     7981 taaaagatga ttttgatgtg tttatcgatt ttacccgtcc ggaaggtacg ctgaatcatc
     8041 tcgctttttg tcgccagcat ggcaaaggga tggtgatcgg cactacgggg tttgacgaag
     8101 ccggtaaaca ggcgattcgt gacgcagccg ccgatattgc gattgtcttt gccgccaatt
     8161 ttagcgttgg cgttaacgtc atgcttaaac tgctggagaa agcagccaaa gtgatggggg
     8221 actacaccga tatcgaaatt attgaagcac atcatagaca taaagttgat gcgccgtcag
     8281 gcaccgcact ggcaatggga gaggcgatcg cccacgctct tgataaagat ctgaaagatt
     8341 gcgcggtcta cagtcgtgaa ggccataccg gtgaacgtgt gcctggcacc attggttttg
     8401 ccaccgtgcg tgcaggtgac atcgttggtg aacataccgc gatgtttgcc gatattggcg
     8461 agcgtctgga gatcacccat aaggcatcca gccgcatgac atttgctaac ggtgcggtaa
     8521 gatcggcgtt gtggttgagt ggtaaggaaa gtggtctttt tgatatgcga gatgtgcttg
     8581 atctcaataa tctgtaacca caaaatattt atagtgatgc aaaaataaca catttaatgc
     8641 attgaatata aagggcttta ttttttagcc ctt
//
LOCUS       DAOTRK010000176         8544 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.177, whole genome shotgun sequence.
ACCESSION   DAOTRK010000176 DAOTRK010000000
VERSION     DAOTRK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.177"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            61..176
                     /gene="rrf"
                     /locus_tag="QIE41_004153"
     rRNA            61..176
                     /gene="rrf"
                     /locus_tag="QIE41_004153"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            231..307
                     /locus_tag="QIE41_004154"
     tRNA            231..307
                     /locus_tag="QIE41_004154"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:265..267,aa:Asp,seq:gtc)
     gene            469..1272
                     /gene="dkgB"
                     /locus_tag="QIE41_004155"
     CDS             469..1272
                     /gene="dkgB"
                     /locus_tag="QIE41_004155"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDS3532963.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLQESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(1269..2183)
                     /locus_tag="QIE41_004156"
     CDS             complement(1269..2183)
                     /locus_tag="QIE41_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3532964.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            2424..3224
                     /locus_tag="QIE41_004157"
     CDS             2424..3224
                     /locus_tag="QIE41_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDS3532965.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3302..4072
                     /locus_tag="QIE41_004158"
     CDS             3302..4072
                     /locus_tag="QIE41_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDS3532966.1"
                     /translation="MTSQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIIVEAHKAA"
     gene            complement(4119..5477)
                     /gene="mltD"
                     /locus_tag="QIE41_004159"
     CDS             complement(4119..5477)
                     /gene="mltD"
                     /locus_tag="QIE41_004159"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDS3532967.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5549..6304)
                     /gene="gloB"
                     /locus_tag="QIE41_004160"
     CDS             complement(5549..6304)
                     /gene="gloB"
                     /locus_tag="QIE41_004160"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDS3532968.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            6338..7060
                     /locus_tag="QIE41_004161"
     CDS             6338..7060
                     /locus_tag="QIE41_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS3532969.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(7057..7524)
                     /gene="rnhA"
                     /locus_tag="QIE41_004162"
     CDS             complement(7057..7524)
                     /gene="rnhA"
                     /locus_tag="QIE41_004162"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDS3532970.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            7589..8320
                     /gene="dnaQ"
                     /locus_tag="QIE41_004163"
     CDS             7589..8320
                     /gene="dnaQ"
                     /locus_tag="QIE41_004163"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDS3532971.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            8453..8529
                     /locus_tag="QIE41_004164"
     tRNA            8453..8529
                     /locus_tag="QIE41_004164"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8487..8489,aa:Asp,seq:gtc)
BASE COUNT         2135 a         2089 c         2073 g         2247 t
ORIGIN      
        1 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgatgaa acagaatttg
       61 cctggcggcc ttagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      121 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ctaggcatca
      181 aatttagcag taagccggtc ataaaaccgg tggttgtaaa agaattcggt ggagcggtag
      241 ttcagtcggt tagaatacct gcctgtcacg cagggggtcg cgggttcgag tcccgtccgt
      301 tccgccactt attaagaagc ctcgagttaa cgctcgaggt ttttttcgtc tgtatatcta
      361 ttattgccag aatcgcaaaa atcctctgca ttttacgctc tttttccgca acagtctgaa
      421 gcccataatc acctcagtta acgaaaatag cattaaaaga ggcatattat ggctatccct
      481 gcatttggtt taggtacttt ccgtctcaaa gacgacgttg ttatttcatc tgtgaaaacg
      541 gcgcttgaac ttggttatcg cgcaattgat accgcacaaa tctatgataa cgaagctgcc
      601 gtaggtcagg cgattgcaga aagtggcgtg ccacgtcatg aactctacat caccactaaa
      661 atctggattg aaaatctcag caaagacaaa ttgatcccga gtctgcaaga gagcctgcaa
      721 aaattgcgta ctgattatgt tgatctgacg ctaatccact ggccgtcacc aaacgatgaa
      781 gtctctgttg aagagtttat gcaggcgctg ctggaagcca aaaaacaagg gctgacgcgt
      841 gagatcggta tttccaactt cacgatccca ttgatggaaa aagcgattgc tgctgttggt
      901 gctgaaaaca tcgctactaa ccagattgaa ctctctcctt atctgcaaaa ccgtaaagtg
      961 gttgcctggg ctaaacagca cggcatccat attacttcct atatgacgct ggcgtatggt
     1021 aaggccctga aagatgaggt tattgctcgt atcgcagcta aacacaatgc gactccggca
     1081 caagtgattc tggcgtgggc tatgggggaa ggttactcag taattccttc ttctactaaa
     1141 cgtaaaaacc tggaaagtaa tcttaaggca caaaatttac agcttgatgc cgaagataaa
     1201 aaagcgatcg ccgcactgga ttgcaacgac cgcctggtta gcccggaagg tctggctcct
     1261 gaatgggatt aagcctctct gacagctcct ccgggagctg tttttacatg ctcgctaagg
     1321 aaatcgataa aagcccggat gcgcgtactt accgcacggt cgctgtaata gactgcactg
     1381 aatggcattt ccactggcaa cactttatct gccattagct ccaccaattc tccgcgagcg
     1441 atttctctgt cgatcatgta gtcggacaaa cacgcaatcc cgttgccact caggcaaagc
     1501 tgtttcagtg tttccccact attggatgac aaaccgtact tcacctcatg taattgtcca
     1561 tcgctacagg ctatcggcca ggtattgagg gaagcgggtt cagtgaatcc caggcaaaca
     1621 tgttgcttta aatcgtcgat cgtttctggc ttcccatagc gggaaatata atcgggggag
     1681 gctataattt ttcgataact gttaaataac ggcctggcac gtaagctgga atccgttaac
     1741 gtaccggcgc gtatcgcgac atccactttt ctttcgatca aattaataat cgtttcggag
     1801 gagactagtg ataaagtgac ttccggatag cgttcacgga aaggcttaat caacggcatc
     1861 agaaagtgca gcactactgg agttgcggca tcgatgcgca acagtccacg cggtgtatta
     1921 cgcgtctcca taatttctga ttctgccgct gccatctcct gcaaaattga ctgtacgcga
     1981 cgaaaataac gctcgccttc ttccgtcagg ctaagttgcc gcgtggtccg attaagcagg
     2041 ctaacgccaa gtttcatctc cagcttcttc accgcccggc ttaccgctga gtttgcttgc
     2101 cctaattgtt ccgctgcccg gctaaagctg ccgctttcga cgaccgaaac aaaaatggcg
     2161 agttcttccg acgtggcttt catttttgct cctgttgcaa aatagatgag atattttgaa
     2221 tttatttgtc attaaaccat caggatgcgt gatatgtcat ccgatttaat gttctcaata
     2281 atgagcaaat ttctgaccgg tgtaagcact tgcttacata acaatataca attgctcgtt
     2341 gaaagagtga gctaaaatcc ctataacagt agaaccctcc cgagtgcgga agggttgacg
     2401 taatagaggt ttcaaagtca aaagtgcgaa aaaacaccta tgccatgcgc tatgttgccg
     2461 gacaacctgc ggaaaggatc ttaccgccgg ggtcttttgc gagcatcggc caggcattac
     2521 cacccggaga accgttaagt accgaagagc gtattcggat cctggtgtgg aacatataca
     2581 aacagcaacg cgctgaatgg ttgtcggtat taaagaacta cggcaaagat gcacatctgg
     2641 tgttattgca ggaagcgcag acaacgccag agttagtaca gtttgcgacc gctaactatc
     2701 ttgccgccga tcaggtaccc gctttcgtgc tgccacaaca tccttctggc gtaatgaccc
     2761 tttcggcggc acatccagtg tattgctgcc cgttacgtga acgagaaccc attttgcgtc
     2821 tggcgaagtc ggcactggtg acggtctatc cattacctga cacccgcctg ttgatggtgg
     2881 ttaatataca cgccgtcaac ttcagtctgg gcgtggatgt ttatagtaag cagttacttc
     2941 ctattggcga tcagatagct catcacagcg gcccggtcat tatggcggga gatttcaatg
     3001 cctggagccg tagaaggatg aacgcgttat atcgctttgc gcgggaaatg tcgctgcgcc
     3061 aggtgcgttt taccgacgat cagcgccgtc gggcgtttgg tcgcccgctc gattttgttt
     3121 tttaccgtgg tctgaacgtc agcgaagctt ctgtactggt tacgcgcgct tccgatcaca
     3181 atccgctact cgttgaattc agtcccggca agcctgataa ataaggtatg tcaggtctgc
     3241 cacagggcag accaacgttt ggcgctgccc ttttatactc aaacaacaaa aggacagcgc
     3301 aatgacatca caatcccacc atgaccacgt agaaaagcag tttagctctc aagctagcga
     3361 atatctgact agtaccgtac atgcatccgg acgggattta cagcgcctgg cggtgcgtct
     3421 ggctgattat cctgatgcaa gtgtacttga tatgggctgc ggagcagggc atgccagctt
     3481 tgtcgctgcg cagaacgtga gcgcggtggt ggcgtatgac ttatctgccc aaatgctgga
     3541 tgtcgtggca caagctgccg aagcccggca actgaaaaat atcaccaccc gccagggtta
     3601 tgtcgaaagt ctgccatttg ccgataacgc atttgatatt gttatcagcc gttattctgc
     3661 ccatcactgg catgatgttg gtgcagcact gcgagaagtg aataggatat tgaaacctgg
     3721 cggtaggctg attgtgatgg acgtaatgtc tccggggcac ccagtgcgcg acgtctggtt
     3781 acagacggta gaagcattac gcgatacctc tcacgtacga aactacgcca gcggtgagtg
     3841 gttgacgtta atcaatgaag ccaatctgat agttgataat ttaattacag ataagttacc
     3901 gctggaattt tcttcatggg tcgcgagaat gcgtacgcca gaagcgttag tagacgctat
     3961 tcgcatttac caacagagcg catcgacaga ggtgaaaacg tattttgcct tgcagaatga
     4021 tggctctttc accagtgata tcatcattgt agaagcacat aaagcagcat aaataaaaaa
     4081 ggcaccgggg gaatcggtgc ctttcattat ctgatttgtc aggaatctgg catgttgttg
     4141 tttttcacaa acaacgtcag cttatcgcct ggttgcagat tcgcagtatc gctgttccag
     4201 cgcatcacat ctttgatgtt cacgccgtgg cgtttagcaa tgcttgaaag cgaatcgcct
     4261 ttgcgcacac gatatgtaat gctatcgctg ttgtttgcca accgctgtgc gctactacct
     4321 gcgccaatcg tcaagctttg gcctggcttc agcttggatc cgcgcagttt gttccactgc
     4381 tgcaaatctt tggtgctaac gccgagacgt gaagcgatac ttgaaagcgt gtcgccagag
     4441 cgtacggtgt aaacacggct gttaagcggc gtattgtcgg caaccagcgt cgactgtaca
     4501 gcagcaattt cgcccgaagc cagagattca cgcagttgat ctgcatgctt ctttggcacc
     4561 atcacgtact gcggaccact tgcgcccagc gtggagcctt tcacgccagc gttgaatgtc
     4621 ttaagcttgc tgacggaaat ccccgccata tctgcaacct tcgccatttc aactgggctg
     4681 ctcaggtgca cacgcgccag agcacggctt tcatcggtcg ttggcagacg tacgccataa
     4741 cgcttgctgt ttttgagaat atcactcaat gccagcattt taggcacgta ctgcttcgtt
     4801 tcctgcggca acggtaacga ccagaagtcc gtggatttcc cacgcgcttt gttcgtttta
     4861 attgccttca tgactcgacc ttcgccgctg ttataagccg ctacggtcag aagccagtcg
     4921 ccgtcaaaca tcttgttcag acgctgcatc atgttcagcg cggcagttgt tgaagcaaca
     4981 acatcgcgac gcgcgtcata attgcgggtc tgtttcaaac cataattgcg ccccgtgctc
     5041 ggaatgatct gccagatgcc tgctgcattg gcgccagacg ttgcgtgagg atcaaaagcg
     5101 ctctccacta tgggtagtag taccagttcc ataggcatgt tacgtttttt aacttgcccg
     5161 gctatccagt acatatacgg ctctgcccgt aaagttacat cgtggagata gctcttattg
     5221 cgtaaatatt tctgtttctg ttcgcgaatc cggtcatttt ccggaattcc catctttagc
     5281 tcgtcgccaa tgaaagccca caagtcacca tctggcgcga tagacgtccc atcgtccatc
     5341 catcgtgcct gacttgtaaa ctttgctgct tccccttgac cagctgcaga aaggctctgt
     5401 gcgtgctgtt gaacgttgcc ggtactctgg caacccacga gcaggacaga ggcgagtaat
     5461 atcgcttttg ccttcatgtg tgtgtcaata gttgcttaaa agacgaccga tcataacggc
     5521 gaacggagcc gatgacaaga aagttttatc agaacctatc tttctttgac cttaaccatg
     5581 caaaacgttc ttcaggttgt tgcaataatg tttcttcatt aattacatta attaaatcaa
     5641 tatcttctgt tcttaaaaaa acattaattt gccgctcatt tttcagaatt acgggtagtg
     5701 ttatttgatt ttttgcccgt aactccttaa ctttacgata ataatcattt atggacaaat
     5761 cgtgcggaag aatactcaaa gcaaacttca tatttgataa ggtatattca tgagcacaac
     5821 ataccaatgt atcgtcaggt aacgcactta actttttaag tgattgatac atttgtgatg
     5881 gtgtcccttc aaacaaccga ccacacccac cagaaaacag cgtgtcgccg caaaatagat
     5941 aaggtttact gaagtaacag atgtgtccta aagtgtgacc cggtgtggca attacactaa
     6001 attcatgccc caaaacgaag gcagtttcgc catctttgac tacctgtgtt gttcccttat
     6061 cttgtgtctc ttgtggacca tacaccacaa tttgcggaaa ctttttcacc agttctttta
     6121 cgccgccaac gtgatcgtgg tgatggtggg tgagaaatat ggcctccggt tgccagttat
     6181 tggcggcaat ggcgtttaat actggctctg cgtctccggg atcgacaatc aggcagcgac
     6241 ctgcttcatc attcaaaacc cagatgtaat tgtcatcaaa ggcgggaata ctgttaagat
     6301 tcatagatta cctctcagtg tgaaacggaa ggttgtgatg aaaccggcaa gagtccctca
     6361 aactgtcgtg gctcctgatt gctggggcga tttgccctgg ggagagcttt atcgcaaggc
     6421 gctggagcgc cagctcaacc cgtggttcac taaaatgtat ggttttcatc tgcttaagat
     6481 tggcaattta agcgcagaaa tcaattgcga agcgtgcgcg gtttctcatc aagtgaatgt
     6541 ttctgcgcaa ggactgcccg tccaggtgca ggcggaccca cttcatcttc cttttgccga
     6601 taaatccgtt gatgtttgtc tactggcaca tacattgccg tggtgcaccg atccgcatcg
     6661 tttattgcgt gaagccgatc gggtattgat tgatgatggc tggctggtga ttagtggctt
     6721 caatcccatc agtttaatgg gattacgaaa acttgtgccg gtattgcgca aaacctcgcc
     6781 ctataatagc cggatgttta ctctgatgcg gcagctggac tggctctctt tgttgaattt
     6841 tgaagtgcta cacgccagcc gtttccacgt tctcccgtgg aacaaacacg gtggaaaact
     6901 attgaatgcg catattcctg cgcttggttg cttacaactt attgtcgccc ggaaacggac
     6961 tattccttta acgctaaatc cgatgaaaca gagtaaaaac aagccacgaa ttcgccaggc
     7021 ggttggagcc acccggcaat gtcgtaaacc acaggcttaa acttcgactt gatagcccgt
     7081 atcttccagt gtgggattca tcgctgcggc acgagccagt tcatcgcaac gttcgttttc
     7141 tggatgaccg gcatggcctt taacccattc ccatttgatt tgatgctgcc ccagcgcagc
     7201 atcaagacgt tgccagagat cgacattttt tactggtttt ttgtctgcgg ttttccagcc
     7261 acgttttttc cagttatgga tccactgggt gataccctgg cggacatact ggctgtcggt
     7321 actcaaaatg acttcgcaat gttcttttaa cgcctccagc gcgacaatag cggccatcaa
     7381 ctccatacgg ttgttggtgg tgcgggtgta gcctgcgcta aaggttttct cgcgtccgcg
     7441 atagcgtaaa atagcgccgt aacccccagg tcctggatta cccagacacg aaccatcggt
     7501 gaaaatttct acctgtttaa gcatctctgg tagacttcct gtaattgaat cgaactgtaa
     7561 aacgataagt ctgacataaa tgaccgctat gagcactgca attacacgcc agatcgttct
     7621 cgataccgaa accaccggta tgaaccagat tggcgcgcac tatgaaggcc acaagatcat
     7681 tgagattggt gccgttgaag tggtgaaccg tcgcctgacg ggcaataact tccatgttta
     7741 tctcaaaccc gatcggctgg tggatccgga agcctttggc gtacatggta ttgccgatga
     7801 atttttgctc gataagccca cgtttgccga agtagccgat gagttcatgg actatattcg
     7861 cggcgcggag ttggtgatcc ataacgcagc gttcgatatc ggctttatgg actacgagtt
     7921 ttcgttgctt aagcgcgata ttccgaagac caataccttc tgtaaggtca ccgatagcct
     7981 tgcggtggcg aggaaaatgt ttcccggtaa gcgcaacagc ctcgatgcgt tatgtgcccg
     8041 ctacgaaata gataacagta aacgaacgtt gcacggggca ttactcgatg cccagatcct
     8101 tgcggaagtt tatctggcga tgactggtgg tcaaacgtcg atggcttttg cgatggaagg
     8161 agagacacaa cagcaacaag gtgaaacaac aattcagcgc attgtgcgtc aggcaagtaa
     8221 gttacgcgtt gtttttgcga cagatgaaga gcttgcggca catgaagccc gtctcgatct
     8281 ggtgcagaaa aagggcggaa gctgcctctg gcgggcataa atacctgtaa aaggcgctaa
     8341 aaatagcgac ttgggcgatt tttgcagcaa acgattcaaa agatgagaaa aaccgttgac
     8401 gaagggtgag gcaatccgta atattcgcct cgttcccaac ggaacacaac gcggagcggt
     8461 agttcagtcg gttagaatac ctgcctgtca cgcagggggt cgcgggttcg agtcccgtcc
     8521 gttccgccaa attcactcat gaaa
//
LOCUS       DAOTRK010000177         8488 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.178, whole genome shotgun sequence.
ACCESSION   DAOTRK010000177 DAOTRK010000000
VERSION     DAOTRK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.178"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(121..4008)
                     /gene="purL"
                     /locus_tag="QIE41_004165"
                     /gene_synonym="purI"
     CDS             complement(121..4008)
                     /gene="purL"
                     /locus_tag="QIE41_004165"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDS3532972.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGLGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAVQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADRESVEAVLAQHGLADCVHYVGQAVSGDHFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            4265..5821
                     /gene="mltF"
                     /locus_tag="QIE41_004166"
     CDS             4265..5821
                     /gene="mltF"
                     /locus_tag="QIE41_004166"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDS3532973.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(5818..6354)
                     /gene="tadA"
                     /locus_tag="QIE41_004167"
     CDS             complement(5818..6354)
                     /gene="tadA"
                     /locus_tag="QIE41_004167"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDS3532974.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(6379..7014)
                     /gene="yfhb"
                     /locus_tag="QIE41_004168"
     CDS             complement(6379..7014)
                     /gene="yfhb"
                     /locus_tag="QIE41_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDS3532975.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            7223..8071
                     /locus_tag="QIE41_004169"
     CDS             7223..8071
                     /locus_tag="QIE41_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDS3532976.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            8127..8387
                     /gene="yfhL"
                     /locus_tag="QIE41_004170"
     CDS             8127..8387
                     /gene="yfhL"
                     /locus_tag="QIE41_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDS3532977.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT         2059 a         2330 c         2176 g         1923 t
ORIGIN      
        1 gtcgtgattt acagacacaa aaaagctccc gaagttggga gcttataata gcggttggtg
       61 cttaatgccg gatgcggcgt gaatgcctca tccgggctgc aaaaccaatg ggctgacgac
      121 ttaccccaac tgcttacgcg cattgcggaa aatgcgcatc catgggccat cctcgcccca
      181 gttttccgga tgccaggagt tgctgacagt acggaaaaca cgttccgggt gcggcatcat
      241 aatggtgact cgaccacttt cagtcgtgac tgccgtaata ccgttcggtg aaccgttcgg
      301 gttagccggg taggtttcag tgactttacc gaagttatcg acatagcgca gtgccaccag
      361 ccctttgctt tccagcgccg ccagatgcgc cgcatcacgc acttccacgc gcccttcacc
      421 atgagagaca gcaatcggca tttgcgagcc caccatcccc tgcaacagca gagacgggct
      481 ttgggttact tcaaccaggc tgaaacgcgc ttcaaagcga tcggaggtat tgcgcacaaa
      541 acgtggccac aactcactac ctgggatcag ttcgcgcagg ttagacatca tctggcaacc
      601 gttacatacc cccagcgcca gcgtttgcgg acgatggaag aaggttgcaa actcatcgcg
      661 tacacggtca ttgaacagga tagacttcgc ccaaccttca ccggcaccca gcacatcacc
      721 gtaggagaaa ccgccgcacg cgaccagggc gtggaaatcc tccaggcccg tgcgtccagc
      781 cagcagatcg ctcatatgca cgtcgatagc atcgaagcct gcacggtgga aagctgccgc
      841 catttcaaca tgcgagttca caccctgctc acgcagcacg gcaactttcg ggcgcgcgcc
      901 agtggcaata aacggtgctg ccacatcttc gttgatatca aacgacagtt tcacattcag
      961 gcccggatcg gcgtcgttgg atttcgcctg gtgctcctgg tcagcacact ccgggttgtc
     1021 acgcaggcgc tgcatctgcc aggtagtttc tgcccaccag acacgcaacg tggtgcggct
     1081 ttcgctaaat acagtctgcc cgttggcagt aatcacaaaa tggtcaccgg aaaccgcttg
     1141 cccaacataa tggacgcaat cagcaagccc atgctgtgcc agtaccgctt caactgattc
     1201 acggtcagcg gctcgaacct gaatcaccgc acccagttct tcgctaaaca atgcagccag
     1261 acgatcgtca cccagagtgg cgatatctgc gtcaatgcca caatgaccag caaaggccat
     1321 ttccgccagc gttaccagca ggccgccatc agagcggtcg tggtacgcga gcagcttacg
     1381 ctgtgctacc agcgcctgaa ccgcgtcata gaaacctttc agttgcgcga cgtcgcgtac
     1441 atctgccggt ttgtcgccaa gctgacgata aacctgcgcc agcgccgttg cgcccagcgc
     1501 gttattgcct ttgcccagat caatcagcag taatgcgtta tcttcggtag aaagctgcgg
     1561 cgtgatggtg tgacgcacat cttccacacg ggcaaatgca gaaatcacca gcgacagcgg
     1621 cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg acatagagtc
     1681 tttacccacc gggatcgtca ggcccagcgc cggacaaagt tcttcgccca cagctttaac
     1741 ggcttcatac aggcccgcat cttcaccagg gtggcctgcc gccgccatcc agttggcgga
     1801 aagtttaatg cgtttgatat cgccaatctg tgttgcggca atattggtta acgcttcacc
     1861 gaccgccaga cgggcagagg cggcgaaatc cagcagcgca accggcgcac gctcgccaat
     1921 cgccatcgct tcgccgtagt agctgtcgag gctggcggta gtgaccgcgc agttagcgac
     1981 cggcacctgc cacggaccca ccatctgatc gcgcgctacc atgccggtta cgctgcggtc
     2041 accaatggtc accaggaaag ttttctccgc cacggtcggc aggtgcagca cgcgtttcac
     2101 cgcgtcagca atggtaatcc cttcacgaac cagcgcatca cccttcgctt tcagcgtttg
     2161 tacttcgcgg gtcatcttcg gcgttttacc aagcagtacg tccagcggca gatcgatcgg
     2221 ctgattatca aaatgacgat cgtgcaaaga aagatgcagt tcttcggtcg cttcaccaat
     2281 caccgcgtat ggcgcgcgct cacgcttaca cagttcgtca aacagcggta actgatcggc
     2341 agcaaccgcc agcacgtagc gttcctggga ttcgttacac cagatttcca gcgggctcat
     2401 gcccggttcg tcgctcagga tatcgcgcag ttcgaattta ccgccgcgcc cgccgtcgct
     2461 caccagttcc ggcatggcgt tagaaagacc gcctgcgcca acgtcgtgga taaacaggat
     2521 tgggttggca tcaccaagct gccagcaacg gtcgatcact tcctggcagc gacgctccat
     2581 ctccgggttg tcgcgctgta cggaagcgaa gtcgaggtcg gcatcagact gaccagacgc
     2641 catggaagac gccgcgccgc cgccaagacc gatgttcatt gccgggccgc cgagaacgac
     2701 cagcttcgca ccaacgttga tctcgccttt ttgtacgtga tcggcacgaa tgttgccgat
     2761 cccgcccgcc agcatgatcg gtttgtgata accgcgcagc tcttcgccat tgtggctgtt
     2821 cactttttct tcataagtac ggaagtagcc gttcagtgcc ggacgaccaa attcgttgtt
     2881 aaatgccgcg ccacccagcg ggccttcggt catgatgtcc agcgcggtga caatgcgctc
     2941 aggcttaccg aaatcttctt cccacggctg ttcgaagcca ggaattcgca ggttggaaac
     3001 ggagaaacca accaaaccag ctttcggctt cgcgccgcgt ccggtggcac cttcatcgcg
     3061 gatttcaccg ccggaaccgg tcgccgcgcc cggccacgga gaaatcgccg tcgggtggtt
     3121 gtgggtttca actttcatca gaatatgcgc cggttcctga tggaaatcgt agcggcccgt
     3181 ttcgtggtca gcaaagtagc ggcccacttc agacccttcc atgacggcgg cgttatcttt
     3241 ataagcagag agaacgtgat ccggcgtagt ttcgaaggtg tttttgatca tcttaaacag
     3301 cgatttcggc tgctgttcac catcgataac ccagtcggcg ttaaaaattt tgtggcggca
     3361 gtgctcggag ttcgcctggg caaacatata cagttcgatg tcgttcgggt tacgaccaag
     3421 ctttgtgaaa gcatcctgca aatagtcaat ttcatcttcc gccagtgcca ggccaagacg
     3481 caggttagcg tcgattagcg cctgacggcc caaccccagc aaatcaacgc tggtgaccgg
     3541 agtcggttga tggtgtgcaa acagctgctc tgcatcatct aaagcaaaaa agaccgtttc
     3601 catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg tcagcgtacc
     3661 ggcttctata tagtaagcca cgccgcgctc aagacggttt acctgttgca gcccgcagtt
     3721 atgggcaata tcggtcgctt tcgaagacca gggagagatg gtgccaggac gcggggtcac
     3781 cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc gggccatatt tcagcaggcg
     3841 ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa aatggacata
     3901 ctcggcgtaa atagtgtgaa ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt
     3961 gattcggaat gccgacaatg caggcgaacc acgcagaatt tccatcataa gtctctcgtc
     4021 ttctaagctt tcggtgtacc caagggggga aacgggcgtc attataaaga atctgatgcg
     4081 ctgacgaaac cgtttgcgtg gaaataaaat caccatcgcg aattagcaac gcgtgccgcc
     4141 aatggctgta ataagttgcc aactggcgca ggtttacgca aaatgccgct catttatgag
     4201 taaacctttc actattatta cgttttttca agctgggacg cgcacgacac agagaattaa
     4261 ctaattgaaa aaattaaaga ttaattatct gttcatcggc attctggcac tgctgctcgc
     4321 ggtcgctctc tggccatcca ttccctggtt tggtaaagcc gacaaccgta tcgccgccat
     4381 tcaagcgcgg ggagagttgc gtgtgagcac cattcatact cccctgacgt ataacgaaat
     4441 caacgggaaa ccttttggcc tggattacga actggcgaaa cagtttgccg attacctcgg
     4501 cgtaaaactg aaagtgaccg tgcggcagaa tatcagccag ctgtttgacg acctcgataa
     4561 tggtaacgcc gacctgctgg cggcaggcct ggtctataac agtgagcgtg taaaaaatta
     4621 tcagcctggc cctacctatt attccgtgtc acaacaactg gtttataaag tgggtcagta
     4681 tcgcccacgt acactgggca acctgacggc ggagcaactc accgttgcac cgggtcatgt
     4741 ggtggttaac gatctccaga ccctgaaaga aacaaaattc ccggaattaa gttggaaggt
     4801 agacgacaaa aaaggctctg cggaattaat ggaagatgtc atcgaaggaa aactcgatta
     4861 caccattgct gattctgttg ccattagcct gtttcagcgc gttcatccgg aactcgccgt
     4921 agcgctcgac attaccgatg aacaaccggt gacctggttt agcccattag atggcgataa
     4981 taccctttcc gccgctctgc tcgatttctt caacgaaatg aatgaagacg gtacgctggc
     5041 acgcattgaa gagaaatacc tggggcatgg cgatgatttt gattacgtcg atacgcgcac
     5101 atttttacgc gccgtcgatg cggtactgcc gcagttaaag cccctgtttg agaaatacgc
     5161 cgaagaaatt gactggcgtt tgctggccgc tattgcttat caggaatcgc actgggatgc
     5221 gcaggccact tcaccgacgg gtgtgcgcgg catgatgatg ctaaccaaaa ataccgcgca
     5281 aagcctcggc attacggatc gtaccgatgc cgaacagagc atcagcggcg gcgtgcgtta
     5341 tttgcaggat atgatgagta aagtgccgga aagtgtgccg gagaacgaac ggatttggtt
     5401 tgccctcgcc gcgtacaata tgggctatgc gcatatgctg gatgcccgcg ctctgacggc
     5461 aaaaaccaaa gggaatcctg acagttgggc tgacgtaaaa cagcgtctgc ctttacttag
     5521 ccagaaaccc tattacagca agctgactta cggctacgct cgtgggcatg aagcctacgc
     5581 ttatgtcgaa aatattcgta agtatcagat tagcctggtg ggttatctgc aggagaaaga
     5641 gaagcaggct acagaagcgg cgatgcaact ggcgcaggat tatccggcgg tatcgcctac
     5701 ggagctgggc aaagagaaat ttccttttct ctcgtttctt tcccagtcgt catcaaacta
     5761 tttgacccac tctccctctc tgctgttttc caggaaaggg agtgaagaga aacaaaatta
     5821 atccgtcgag gattgcgctt ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa
     5881 gtcactgagc aacgccgcgc actcatccgc cagtattcct tcggtaattt ccactcggtg
     5941 attcataccc ggatgatgca gcacatccat taaagatccc gcagcgccag ttttcgcgtc
     6001 acgcgcacca aagaccacgc gaccaatgcg actgtggatc atcgctccgg cacacattac
     6061 acagggttca agcgtgacat acaacgtggc gtcgatcaga cgataatttt gcatcaccag
     6121 accgccctgc cgcagagcca tgatttctgc atgtgcggtg ggatcatgtc gaccaatcgg
     6181 acggttccag ccttcgccaa taacacgatt gttatgcact aataccgcgc cgaccggcac
     6241 ttcccgctca tcccaggcac gctttgccag cgtcagcgcg tgacgcatcc agtattcgtg
     6301 gctaaattcg acttcagaca aaaagaaaac tccggttata aaagcgcggc gcattataca
     6361 cggacgctat gctttactct attccagttg ctggagttca ccgcgcgggg ttactcgcca
     6421 acgatgctga cagaaataaa gcagcgggtt gtcctgatta ctgtcgctat agccactgta
     6481 caaccgcagt ggcgtgccga ttttacgctc cagttgcgcg accttctcat gcccgaggca
     6541 acgcatcgtc aatacccaac caccatagcc acgctgaatc tgactggcga taagattaac
     6601 ccgcggcagc cagggcgtat cgaaataaac cgcttcaacc agcggctgcg gagagccggt
     6661 aatcaaccag atatcagcat cggaacttaa caggtaggtg gttaatcgct cctgaaccag
     6721 cggaaaggcg gtaacattgt cacgaaacca gcgtacgaaa tcggcctgca acgcctgcaa
     6781 acgcgcttca ttgtgaccaa aagtgcatcc ccacagaagc agactcatcg gccagcgtgc
     6841 cgcacggcct tttatcaata acgcaatggc tatgatcggc aacaacggca ggacaagcaa
     6901 cgcattcagc ggttggcgcc gcagtaaata acgcaaaaaa ctgccgaaca tatcctgctg
     6961 atgcaatgtt ccatctaagt caaaaaacac cacacgacgc tcgtgagttg ccaaaccata
     7021 ctcctctgga ctgaaacatc ctgattaatt ttcgctacta catagcctaa cagatagatc
     7081 atcacttttc cggcaacaat cgaccgcatc atggctaact ggaattttta attcatggaa
     7141 attagcggca atggaatata aaattcactc gcgtttgtct catattgcct gttgtcgcca
     7201 ctatttcatc gcgaggaaag atatgaacgg cttacttcgt atccgtcagc gttaccaggg
     7261 gcttgcccag agtgataaaa aactggcgga ttatctgctg ctacaacctg atacggcgcg
     7321 ccatttaagc tctcagcaac tggccaacga agccggagtc agtcagtcca gcgtcgtgaa
     7381 gttcgcgcaa aaactcggct ataaaggttt tccggcgctt aagttggcgt tgagtgaagc
     7441 gctggcaagc cagccggaat cgccctccgt gcccattcat aaccaaatcc gcggtgatga
     7501 tccgttacgg ctggtcggcg aaaaactgat taaagaaaat actgccgcta tgtacgcaac
     7561 gctaaacgtt aatagtgaag agaaactgca tgaatgcgta acaatgttgc gctctgcgcg
     7621 gcggataatt ctgaccggta ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa
     7681 gctgatgaag attggcttca atgctgccgc agtgcgcgat atgcatgcgc tactcgctac
     7741 cgtacaggcg tcgtcccctg acgatctgtt attagccatt tcttacaccg gtgtacgacg
     7801 cgagttaaac ctggcggcag atgagatgct gcgagtgggc ggaaaagtgc tggcgattac
     7861 cggctttact ccgaatgccc tgcaacagcg tgcttctcat tgcctgtata ccattgccga
     7921 agaacaagcg acaaacagtg cttcaatctc tgcttgtcac gcgcagggaa tgttaacgga
     7981 tttgctgttc attgcgctga ttcagcagga tctggaactg gctccagaac gtattcgtca
     8041 cagtgaagcg ctggtgaaaa aactggtctg agtaaagaat gcgcgtataa tgcccgcccg
     8101 gtttgtgttg ttttgagagt ttccttatgg cgctgttaat cactaaaaaa tgcatcaatt
     8161 gtgatatgtg tgaacccgaa tgcccgaatg aggcgatttc aatgggcgat catatctatg
     8221 agattaacag cgataagtgt accgaatgcg tagggcacta cgagacacca acctgccaga
     8281 aggtgtgccc gatccccaat actattgtga aggatccgca acatgtcgag acggaagaac
     8341 agttgtggga taaatttgtg ctgatgcatc acgcggataa aatttaatat tctgctcctg
     8401 gagtagacta ttgttgactt tatagtgtga gccttcaggt tcttatattt attttcgtga
     8461 tgtgatcata aatatcaatt atttcatg
//
LOCUS       DAOTRK010000178         8425 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.179, whole genome shotgun sequence.
ACCESSION   DAOTRK010000178 DAOTRK010000000
VERSION     DAOTRK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.179"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..139
                     /locus_tag="QIE41_004171"
     CDS             <1..139
                     /locus_tag="QIE41_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS3532978.1"
                     /translation="SGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGL
                     S"
     gene            410..2647
                     /gene="nrfB"
                     /locus_tag="QIE41_004172"
     CDS             410..2647
                     /gene="nrfB"
                     /locus_tag="QIE41_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDS3532979.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2634..5606
                     /gene="nfrA"
                     /locus_tag="QIE41_004173"
     CDS             2634..5606
                     /gene="nfrA"
                     /locus_tag="QIE41_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDS3532980.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDARARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLISKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATHNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYSIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5607..6497
                     /locus_tag="QIE41_004174"
     CDS             5607..6497
                     /locus_tag="QIE41_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDS3532981.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6680..7441
                     /gene="envY"
                     /locus_tag="QIE41_004175"
     CDS             6680..7441
                     /gene="envY"
                     /locus_tag="QIE41_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDS3532982.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7487..7563)
                     /locus_tag="QIE41_004176"
     tRNA            complement(7487..7563)
                     /locus_tag="QIE41_004176"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7527..7529),aa:Arg,seq:tct)
     gene            7806..>8425
                     /gene="fimZ"
                     /locus_tag="QIE41_004177"
     CDS             7806..>8425
                     /gene="fimZ"
                     /locus_tag="QIE41_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDS3532983.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNNELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKL"
BASE COUNT         2110 a         2154 c         2144 g         2017 t
ORIGIN      
        1 ttcagggata cggcacatcg ctattaatat tttgacgaat gataaggtat tcaaggcagg
       61 gttaagacgt aagatgcgaa aagcagccat ggacagaaac tatctggcgt cagtccttgc
      121 ggggagcggg ctttcgtaat cttgccctgc ctgctcacct cctccaggca cttccactac
      181 atgatgacct gctcggtgcc accgctcctt ttcactatga cgttaacctc cctgtcccag
      241 gacaccatgc ttttgtctac attctacgca aacggcaact tttataacca gtgtaaaaat
      301 aacgtgccgc aatagtcata cctattaatg gtaaaaagct gtcacaattc ataaaaaacc
      361 ttaatatacg ccaccctaaa cataaccagc gttaatgtaa ggtttttgtg tggactggct
      421 tcttgatgtt tttgctacct ggctctacgg cttaaaagta atcgcgataa cgttagcggt
      481 catcatgttc atcagcgggc tggacgattt ttttattgat gtcgtctact gggtacgccg
      541 cattaaacgc aagttgagtg tttatcgccg ctacccgcga atgagttacc gcgaactgta
      601 taaaccagat gaaaaaccgt tagcgattat ggttccggcg tggaatgaaa cgggcgtcat
      661 cggcaatatg gccgagctgg cggcgaccac gctcgactat gaaaactatc atatctttgt
      721 tggcacctac cccaacgacc ccgatactca gcgtgatgtt gacgaagtgt gcgctcgctt
      781 cccgaatgtg cataaggtag tctgcgcgcg acctggcccc accagcaaag ccgactgcct
      841 gaacaacgtg ctggacgcca tcacccagtt tgagcgtagc gccaatttcg cttttgctgg
      901 ttttattctg catgacgccg aagatgtgat ttcaccgatg gaattgcgtc tgttcaacta
      961 tctggtcgag cgtaaagatc tgattcagat cccggtgtat ccgttcgaac gcgaatggac
     1021 gcacttcacc agcatgactt acattgatga gttttcagaa ctgcatggca aagatgttcc
     1081 ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc gtcggtacct gtttcagccg
     1141 ccgcgccgtg accgcactgt tagctgacgg tgacggtatt gctttcgacg tgcagagtct
     1201 tactgaagat tacgacattg gcttccgcct gaaagaaaaa ggtatgacgg aaatttttgt
     1261 ccgttttccg gtggtggacg aagccaaaga acgcgagcag cgtaaatttt tacagcacgc
     1321 gcggacgtca aacatgatct gcgtgcgcga atatttcccc gatacctttt cgactgcggt
     1381 tcgacaaaaa tctcgctgga tcatcggcat tgttttccag ggctttaaaa cccacaaatg
     1441 gacctccagt ctgacgctga actactttct ctggcgcgac cgcaaagggg caatcagtaa
     1501 ctttgtcagc ttcctcgcga tgctggtgat gatccagctt ttgctgttgc tggcgtatga
     1561 aagtttgtgg cccgatgcct ggcatttcct ttctattttc agcggcagcg catggttaat
     1621 gactctgctg tggctaaact ttggcttgat gattaaccgc atcgtgcagc gggtgatttt
     1681 cgttactggc tactacggcc tgacgcaggg gctgctatct gtcctgcgtc ttttctgggg
     1741 caacctgatt aacttcatgg ccaactggcg cgcgttaaaa caggtacttc aacacggcga
     1801 tccacgtcgt gtcgcgtggg ataaaacaac gcatgacttc cccagcgtga ctggcgatac
     1861 ccgctcgttg cgtccgttag gtcaaatcct gctggaaaat caggtcatca ctgaagaaca
     1921 actcgataca gcactgcgta atcgcgtcga aggtctacgc ctgggcggtt caatgctgat
     1981 gcaggggctg attagcgccg aacagctggc acaggcgctg gcagagcaaa acggcgtggc
     2041 gtgggagtcg atcgacgcct ggcagatccc ttcatcactg attgccgaaa tgccggcttc
     2101 cgtggcgctg cattatgcag tactgccgct gcgtctggaa aatgatgagt taattgtcgg
     2161 cagtgaagat ggtattgacc cggtttcgct ggcggccctg acgcgtaaag tcggacgcaa
     2221 agtgcgttac gtcattgttc tgcggggaca aattgtcacg gggttacgcc actggtatgc
     2281 gcgccgacgc ggtcacgatc cgcgggcaat gttgtacaat gcggttcagc atcagtggct
     2341 cacggaacag caggccggtg aaatctggcg gcaatatgtg ccgcatcagt tcctgttcgc
     2401 cgaaatactg accacgctcg gtcatattaa tcgttcagca attaacgtgt tgttattgcg
     2461 ccatgaacgc agttctctgc cgctcggcaa gtttttggtc accgaaggcg ttatcagcca
     2521 ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta caagtttcga tgcaatcact
     2581 attactcaaa gcaggtttaa acacagaaca ggttgcgcaa ctggagtccg aaaatgaagg
     2641 agaataacct taatcgcgtc atcggatggt ctggtttact gctgacgtct ttattgagta
     2701 ccagcgcact cgcagacaat atcggcacca gcgcagaaga gctggggctg agcgattatc
     2761 gccattttgt tatttatccc cgtctcgaca aggcgctgaa ggcacagaaa aataacgacg
     2821 aagcaaccgc catccgcgaa tttgaatata tacaccagca ggtgccggat aatattccgc
     2881 taactttata ccttgctgaa gcctatcgcc attttggtca tgatgcccgg gcgcggctgt
     2941 tgcttgagga tcaactgaaa cgtcacccag gagatgcccg acttgagcgc agtctggcgg
     3001 ctattccagt tgaagtgaaa agcgttacga ctgttgaaga actgcttgcc cagcaaaaag
     3061 cgtgcgatgc tgcgccgacc ctgcgttgtc gcagtgaagt tgggcagaat gccctgcggc
     3121 tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc gacgtttgct gcatcgccgg
     3181 aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat ctacctgaag caatggtccc
     3241 aggcagatac gctatacaat gaagcacgcc agcagaacac attaagcgcg gcagaacgcc
     3301 gccagtggtt tgatgtgctt cttgccgggc agctggacga tcggatcctg gcgctgcaat
     3361 cacaggggat cttcaccgat cctcagtcat atattactta cgcgaccgcg ctggcttatc
     3421 gtggcgaaaa agcgcgcctc cagcattatc tcattgaaaa taagccgctg tttaccacgg
     3481 acgcacaaga gaaaagttgg ctctatctga tatctaaata cagcgccaac cccgttcagg
     3541 cgttggcgaa ttatacggta cagtttgccg ataaccgcca gtatgttgtt ggcgcgacgc
     3601 taccggtgct gttaaaagaa ggtcagtacg acgcagcgca aaaactgctc gccaccctcc
     3661 ccgccaatga aatgcttgag gagcgttata ccgttagcgt agcgacccat aacaaggctg
     3721 aagctctgcg tctggcacga ttgctgtatc agcaagaacc ggcaaatctt acccgcctgg
     3781 atcaactgac ctggcaactg atgcagaacg agcagtcacg cgaagctgcc gatttattgc
     3841 tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca gacgttaatg gcgcgactgg
     3901 cgtctctgct ggaaagtcat ccttacctgg caacgccggc gaaggtggcg attttatcga
     3961 aacccttacc gctggcggag caacgtcagt ggcaaagtca gttgccgggt attgcagata
     4021 attgcccggc tatagttcgc ttgctgggcg atatgtcgcc ttcctacgat gccgccgcct
     4081 ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg tgtggcgttg tatgcatggc
     4141 ttcaggccga acaacgacaa ccgaacgcct ggcaacatcg tgcggtagcc tatcaggcgt
     4201 atcaggttga ggactacgcc accgcactgg cggcctggca gaaaatcagt cttcacgaca
     4261 tgagcaatga ggatctgctt gctgctgcca ataccgccca ggcggcagga aatggtgcgg
     4321 ctcgcgatcg ctggcttcag caggcagaac aacgtgggct gggaaacaat gccctctact
     4381 ggtggctgca tgcgcaacgt tacattcctg gtcagccgga actcgcactg aacgatctca
     4441 cgcgctcaat caatattgcg ccttctgcca acgcttacgt tgcgcgggcg acaatttatc
     4501 gccaacgtca taatgtcccg gcggcggtga gtgatttgcg cgccgcgctg gaactggaac
     4561 cgaataatag caacacccag gcagcgctcg gttacgcctt gtgggatagc ggtgatatcg
     4621 cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct accggacgat ccggcactta
     4681 tccgacaact ggcctacgtg aaccagcgtc tggatgacat gcctgcgacg cagcactacg
     4741 cccggctggt gattgatgac attgataatc aggcgctgat aaccccactg accccagagc
     4801 aaaatcagca acgcttcaat ttccgccgtc tgcatgagga ggtcggtcgc cgctggacgt
     4861 tcagtttcga ttcttctatc ggcttgcgtt ccggcgcaat gagtaccgct aacaataatg
     4921 tcggcggcgc agcgccaggg aaaagctatc gtagctacgg gcaactggaa gccgagtaca
     4981 gcatcggacg caatatgctg ctggaaggcg acctgctctc ggtttacagc cgtgtatttg
     5041 ccgatactgg agaaaacggg gtgatgatgc cggtgaaaaa tccgatgtcc ggcaccggtc
     5101 tgcgctggaa gccgctgcgc gatcagatct ttttcctcgc cgtcgaacag cagttgccgc
     5161 tgaacgggca aaatggcgca tccgatacca tgctgcgcgc cagcgcctca ttctttaatg
     5221 gcggcaaata cagcgacgaa tggcacccga acggttcagg ctggtttgcc caaaacctgt
     5281 acctcgatgc agcgcaatat atccgccagg atattcaggc gtggacggca gattatcgcg
     5341 tcagctggca tcagaaggta gctaacggac agactattga gccttacgct cacgttcagg
     5401 acaacggcta tcgtgataaa ggcactcagg gcgcgcagct tggcggtgtc ggggtccgct
     5461 ggaatatctg gaccggcgag acgcactacg acgcctggcc gcacaaagtc agtctcggcg
     5521 tcgagtatca acataccttt aaggcgatta atcaacgtaa cggagagcgc aacaatgcgt
     5581 ttctcaccat tggagtgcac tggtaaatgc gtaagttcat tttcgtattg ctgacactgc
     5641 ttttggtcag ccctttttcc tttgcgatga aaggtattat ctggcaacca caaaaccgtg
     5701 atagtcaggt taccgatacc cagtggcagg ggctgatgag tcagttacgt ttgcaaggct
     5761 tcgataccct ggttttgcaa tggactcgtt acggcgatgc attcacccag ccagaacagc
     5821 gcgcgttatt gtttaagcgt gccgcagctg cgcaacaggc tggcctgaag ctgattgtcg
     5881 ggctgaatgc cgatccggaa ttttttatgc accagaaaca gtcatccgcg gcgctggaaa
     5941 gctatcttaa tcgcctgctg gctgccgatc tccagcaagc cagattatgg agcgccgtac
     6001 ctggcgtaac gccggatggc tggtacatca gcgcggaaat tgacgacctg aactggcgca
     6061 gcgaagccgc ccgtcagcct ttgctgacat ggttaaacaa cgcgcagcgg ttgattagcg
     6121 atgtttcagc aaaaccggtt tatatcagta gttttttcgc cggaaacatg tcgcccgatg
     6181 gctatcgcca actgctggaa cacgttaaag caaccggcgt taatgtctgg gtacaggatg
     6241 gcagcggcgt ggataaactt accgctgaac agcgtgaacg ttatctacag gccagcgccg
     6301 attgccaaag ttccgcccct gccagcggca ttgtttatga actgtttgtc gccggcaaag
     6361 gcaaaacctt tacagcgaaa ccgaaaccgg atgcagagat cgcctcgctg ttagcgaaac
     6421 gttcctcttg cggtaaagac acgctctatt tctctctgcg ctatttgccc gtcgcgcacg
     6481 gcattctcga gtattaaatc tccttcaggt aagtcgggta cgacctggct tacctctttc
     6541 gctctcgtca gaattccatc aagattaatt gctaaaaagc gtgctaaaga aagaagatcg
     6601 ttatcggcga acgggccgat cactcatgca ggaagcatag atcctttgag cggagtttct
     6661 gatatctgag gagtgcgaaa tgcaattgag cagcagtgaa ccttgcgtgg tgatcctgac
     6721 cgaaaaagag gtagaggtaa gcgtcaataa ccatgccacg tttacccttc cgaaaaatta
     6781 cctggccgcc ttcgcgtgca acaataacgt cattgaactc tcaacgttaa atcacgtatt
     6841 aatcacccac atcaaccgca acatcatcaa cgattatctg ttgtttttaa ataagaactt
     6901 aacctgtgta aagccctggt cgcggctggc aaccccggtt atcgcttgtc atagccgtac
     6961 accggaagtg ttccggctag ccgccaacca cagcaagcag caacccagca aaccctgcga
     7021 agcggagttg acgcgcgcat tgctttttac cgtattgtct aactttcttg agcaatcgcg
     7081 gtttattgcc ctactgatgt atatcttacg cagcagcgtc cgcgacagcg tctgccgcat
     7141 tattcaaagc gatattcagc attactggaa tctgcgaatt gtcgccagtt cgctatgttt
     7201 aagccccagc ctgctcaaaa agaaattaaa aaacgaaaat accagctata gccagattgt
     7261 cacagagtgt cgtatgcgtt acgccgtaca gatgttattg atggataaca aaaatatcac
     7321 tcaggtagcg caattatgtg gctatagcag cacgtcgtac tttatctctg tttttaaggc
     7381 gttttacggc ctgacaccgt tgaattatct cgccaaacag cgacaaaaag tgatgtggtg
     7441 aagggcaaag cggaaacgga taagacgggg ataaatgaag aagaaatggc gcgccctgca
     7501 ggattcgaac ctgcggccca cgacttagaa ggtcgttgct ctatccaact gagctaaggg
     7561 cgcgttgata ccgcaatgcg gtgtaatcgc gtgaattata cggtcaaccc ttgctgagtc
     7621 aatggctttt gatctggttg ctgaacaagt gaacgaccgc gtctgatttt ctgatttatt
     7681 tcgctatagc ggcaaacaaa cgcacatcgc tgcgcgtctg aatcaagaaa acccgtattt
     7741 tcatgtatca aagtacaatt tcccgcccta acggaaaatt gtccgatcca atgagactgg
     7801 taactatgaa accaacgtcg gtgatcatta tggatactca tcctatcatc agaatgtcta
     7861 ttgaagttct gttgcaaaaa aacaatgaat tgcaaattgt cctgaaaacg gatgattatc
     7921 gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg gatatagact
     7981 tgcccggaac agacggtttt accttcctga aaaggatcaa acaaatccag agcacagtga
     8041 aagtattatt tttatcatcg aaatcagaat gcttttatgc tggcagagcg atacaagctg
     8101 gtgctaacgg ttttgtcagt aaatgcaatg atcaaaatga tatttttcac gccgttcaga
     8161 tgatcctctc cggatacact tttttcccca gcgaaacgct caactatata aaaagcaata
     8221 aatgcagtac gaatagttca acggtcacgg tgctatctaa tcgtgaagtg accatattac
     8281 gttatctggt tagcggatta tccaataaag aaattgccga taagttatta cttagcaata
     8341 aaacagttag tgcgcataaa tctaatattt atggcaagct aggtttgcat tcaattgtag
     8401 agcttatcga ctatgccaaa ttata
//
LOCUS       DAOTRK010000179         8394 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.180, whole genome shotgun sequence.
ACCESSION   DAOTRK010000179 DAOTRK010000000
VERSION     DAOTRK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.180"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..206)
                     /locus_tag="QIE41_004178"
     CDS             complement(<1..206)
                     /locus_tag="QIE41_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDS3532984.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSEKPVWMDQAEKATGDFY"
     gene            519..608
                     /gene="kdpF"
                     /locus_tag="QIE41_004179"
     CDS             519..608
                     /gene="kdpF"
                     /locus_tag="QIE41_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDS3532985.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            608..2281
                     /gene="kdpA"
                     /locus_tag="QIE41_004180"
     CDS             608..2281
                     /gene="kdpA"
                     /locus_tag="QIE41_004180"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDS3532986.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTAPGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2304..4352
                     /gene="kdpB"
                     /locus_tag="QIE41_004181"
     CDS             2304..4352
                     /gene="kdpB"
                     /locus_tag="QIE41_004181"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDS3532987.1"
                     /translation="MSRKQLALFEPTLAVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCICIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVKAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKMLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSHVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4361..4933
                     /gene="kdpC"
                     /locus_tag="QIE41_004182"
     CDS             4361..4933
                     /gene="kdpC"
                     /locus_tag="QIE41_004182"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDS3532988.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFSGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4926..7610
                     /gene="kdpD"
                     /locus_tag="QIE41_004183"
     CDS             4926..7610
                     /gene="kdpD"
                     /locus_tag="QIE41_004183"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDS3532989.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7607..8284
                     /gene="kdpE"
                     /locus_tag="QIE41_004184"
     CDS             7607..8284
                     /gene="kdpE"
                     /locus_tag="QIE41_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDS3532990.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDITVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1756 a         2177 c         2447 g         2014 t
ORIGIN      
        1 cagtaaaaat caccagttgc cttttcggcc tgatccatcc acaccggttt ctcgctggtt
       61 ttcgaccaga cgcggtgcag gtagctgtaa aagcgggcgc ggtctttcca gaaaagcatg
      121 aaaggcagcg ccagaacgcc cgctgcaacc gcataagtac ggcgtaaaaa gataagccat
      181 gcaggatatt ctctgtagag ttccatactt ttctccccct tttgtatcta cccggtgaat
      241 ggcaccggaa aaatgaattt gtttatctga tgaaaatagt accgcctttt gtgtaatttt
      301 actactcatc cgaccactta tttttgctta ttgatggttt attgacattc acactgtaat
      361 taagttacac aaaagttaaa ttaatactaa ccatcagtta aatcatggct tttgccattt
      421 ttatactttt tttacacccc gcccgcagat ttttgcgaaa tctttgcagc cagaattcta
      481 cccttccggt atcactttta ggccactgga ggtgcactgt gagtgcaggc gtgataaccg
      541 gcgtgttgct ggtgttttta ttactgggtt atctggttta tgccctgatc aatgcggagg
      601 cattctgatg gctgcgcaag ggttcttact aattgccacg tttttactgg tattaatggt
      661 gctggcgcgt cccttaggta gcgggctggc gcggctgatt aatgatattc ctcttcctgg
      721 tacgacgggc gttgaacgcg tactctttag cgcgcttggc gtctctgacc gcgagatgaa
      781 ctggaagcag tatctctcag ccattcttgg cctgaatata ctggggctgg cggtgctgtt
      841 ttttatgttg ctcggtcagc actatctgcc gcttaatcca cagcagttgc cggggctgtc
      901 gtgggatctg gcgctgaata ccgccgtcag ctttgtcacg aataccaact ggcaatctta
      961 cagcggtgaa accacgttga gctatttcag ccagatggcg ggcttaacgg tgcaaaactt
     1021 tctttctgcc gccagcggga ttgcggtgat ttttgccctc atccgtgcgt ttacccgcca
     1081 gagcatgagc acactcggga atgcctgggt cgatctgtta cgcatcacgt tatgggtgct
     1141 agtccctgtg gcgttgttga ttgcattgtt ttttattcaa caaggtgcgt tgcaaaactt
     1201 tctgccctat caggcagtga ataccgttga aggagcgcaa cagctgttac caatgggtcc
     1261 ggtggcttct caggaagcga tcaagatgct cggcactaac ggtggtggct tctttaatgc
     1321 caactcgtcg catccgtttg aaaacccaac cgcactgacc aacttcgtgc agatgctggc
     1381 gatcttcttg atcccaacag cgctgtgctt tgcctttggt gaagtggcgg gcgatcgccg
     1441 ccaggggcgc atgttactgt gggcgatgtc agtgattttt gtcatctgcg taggcgtggt
     1501 gatgtgggca gaagttcagg gtaatcctca tctgctggca ctgggcgcgg acagcagcat
     1561 caatatggaa ggtaaagaga gccgtttcgg cgtgctggtc agtagcctgt ttgcggtcgt
     1621 gactacggcg gcttcctgcg gcgcggtgat tgcgatgcat gattcgttta ccgcccccgg
     1681 tggcatggtg ccgatgtggc tgatgcaaat tggtgaagtg gtattcggcg gtgtcggttc
     1741 tggtctttac ggcatgatgc tgtttgtcct gctggcggtg tttattgccg ggctgatgat
     1801 tggtcgtaca ccggaatatc tgggtaaaaa aatcgacgta cgcgagatga aactgaccgc
     1861 actggcgatt ctggtcaccc cgacgctggt gctgatgggc gcggcgctgg caatgatgac
     1921 cgacgccgga cgtagcgcca tcctcaaccc gggcccgcat ggttttagcg aagtgctgta
     1981 cgccgtgtcg tccgccgcta acaacaacgg cagcgccttt gccggattaa gcgccaactc
     2041 cccattctgg aactgtttgc tggcgttctg catgtttgtt ggtcgcttcg gggtgattat
     2101 tccggtgatg gcaattgctg gttcgctggt gagtaaaaag agccaacccg ccagctccgg
     2161 cacactgcct acgcacggcc cgctgtttgt tggcctgtta atcggcaccg tgttgctggt
     2221 tggcgcactg acctttatcc ctgccctggc gcttggcccg gtggcggaat atctctcctg
     2281 atgatattga gtgagcactg aatatgagtc gtaaacaact ggcgctattc gaaccaacac
     2341 ttgccgttca ggcgctgaaa gaagcggtga aaaaattaaa cccgcaggca caatggcgca
     2401 atccggtgat gtttatcgtc tggatcggca gtttgctgac cacctgtatt tgcatcgcaa
     2461 tggcaagcgg tgcgatgcct ggcaatgcgc tgtttagcgc ggcaattagc ggctggctgt
     2521 gggtcaccgt actgttcgct aatttcgccg aggcgctggc agaaggccgc agtaaagcgc
     2581 aggccaacag tctgaaaggg gtgaaaaaaa ctgcctttgc ccgcaagctg cgtgagccga
     2641 aatatggcgc tgcggcggac aaagttcccg ccgatcaact gcgtaaaggc gatatcgtac
     2701 tggtgaaagc tggcgatatt atcccctgcg atggtgaagt cattgaaggg ggcgcatcgg
     2761 tcgatgaaag cgccatcacc ggggaatcgg caccggtgat ccgtgaatcc ggcggcgatt
     2821 ttgcctccgt cactggcggc acgcgtattc tttctgactg gctggtgatt gagtgcagcg
     2881 ttaaccccgg cgagacattt ctggatcgga tgatcgcgat ggtggaaggc gcgcagcgac
     2941 gtaaaacgcc aaacgagatt gccctgacca tcctgctgat tgccctgact atcgtctttt
     3001 tactggcaac cgccacgctg tggccgtttt ccgcgtgggg cggtaatgca gtcagtgtaa
     3061 cggtactggt ggcgctgctg gtctgtctga tcccaacgac cattggcggt ctgttgtcag
     3121 cgatcggcgt cgccgggatg agtcggatgc tgggcgcgaa tgtgattgcc accagcggac
     3181 gtgcagttga agcggcgggt gacgttgacg tcctgctgct ggataaaacc ggcaccatca
     3241 cgctaggtaa ccgtcaggca tcggagttta tccctgcgca gggcgtggat gaaaaaatgc
     3301 tggctgacgc cgcacaactg gcttcactgg ctgatgaaac gccggaaggt cgcagtattg
     3361 tgatcctcgc caagcagcgt tttaacctgc gcgagcgcga tgtgcagtcg ctccatgcca
     3421 cctttgtacc gtttactgcg caaagccgga tgagcgggat caacatcgac aaccgcatga
     3481 tccgtaaagg ttctgtcgat gccattcgcc gccatgttga ggctaatggt ggtcacttcc
     3541 ctgccgatgt tgatcaaaaa gtcgatcagg ttgcgcgtca gggagccacg ccgctggtag
     3601 tagtagaagg ctcccatgtg cttggcgtta ttgcgctgaa agatatcgtc aaaggcggta
     3661 ttaaagagcg cttcgcgcag ttgcgtaaaa tgggcattaa aacggtgatg attaccggcg
     3721 ataaccgtct gactgccgcc gcgattgctg cggaagcggg cgtcgatgat ttcctcgccg
     3781 aagcgacacc agaagccaag ctggcattga ttcgtcagta tcaggcagaa ggccgtttag
     3841 tggcgatgac cggcgacggc accaacgatg ctccggcgct ggcgcaggca gatgtcgcgg
     3901 tggcgatgaa ctccggtact caggcggcga aagaagcggg caatatggtg gatctcgact
     3961 ctaacccgac caagttgatc gaggtggtac atattggcaa acagatgctg atgacccgtg
     4021 gctcgctgac caccttcagc attgccaacg atgtggcgaa atacttcgcc attattccgg
     4081 cggcattcgc ggcaacgtat ccgcagttaa atgcgctgaa catcatgcgc ctgcattcgc
     4141 ccgactccgc aatcctcagt gcggtgattt tcaacgcctt gattatcgtc tttttgattc
     4201 ccctggcgtt aaaaggcgtg agttataaac cgcttaccgc ttctgccatg ttgcgccgta
     4261 acttatggat ttacggtctg ggtgggctgc tggtgccgtt tatcggtatc aaagtcattg
     4321 atttactgct gaccgtttgc ggtctggtgt gaggtttacc atgcgtggat tacgtccggc
     4381 attatcgaca tttctctttt tgttactgat tactggcggc gtttacccgc tgctgaccac
     4441 cgcgctgggg caatggtggt ttccctggca ggccaacggt tcgttgattc gtgaaggtga
     4501 tacggtgcgc ggttcggcat taatcgggca gaatttttcc ggcaacggct attttcatgg
     4561 tcgcccgtcg gcaacggcag aaatgcccta taatccacag gcttctggcg ggagcaatct
     4621 ggcggtcagt aaccctgagc tggataaact aatagccgca cgcgttgctg cattacgggc
     4681 cgctaacccg gatgccagcg cgaacgttcc ggttgaactg gtgacggcat cggcaagcgg
     4741 gctggacaat aacatcaccc cgcaagcggc ggcctggcag atcccacgcg tggcgaaagc
     4801 gcgtaatctc agcgttgaac agctcacgca actgatcgca aaatacagcc aacaaccgtt
     4861 ggtgaaatat atcggccagc cggtcgtcaa cattgtcgaa ctcaatctgg cgctggataa
     4921 gcttgatgaa taacgaaccc ttacgtcccg accccgatcg tctgctggaa caaactgccg
     4981 cgccgcatcg ggggaagctg aaagttttct tcggtgcctg tgcaggcgtc gggaagacct
     5041 gggcgatgct ggcagaagcc cagcgactgc gggcgcaagg gctggatatt gtggttggcg
     5101 tggtagaaac ccacgggcga aaagataccg ccgccatgct ggaagggctg gctgttctgc
     5161 cgttaaaacg ccaggcgtac cgtgggcggc atatcagcga gtttgatctc gatgccgccc
     5221 tcgcccgccg tccggcgctg atcttaatgg acgaactggc gcacagtaat gcgccaggtt
     5281 ctcgtcatcc taaacgctgg caggatatcg aagaactgct ggaagctggc attgatgttt
     5341 tcacaaccgt caacgttcag catctggaaa gtctgaatga tgtggtcagc ggcgtcaccg
     5401 gaattcaggt tcgggaaacc gtgcccgatc cttttttcga tgccgccgac gacgtggtgc
     5461 tggtggactt gcccccagac gatctgcgcc agcggctgaa agaaggcaaa gtctatattg
     5521 ccgggcaggc ggagcgcgcc attgaacatt ttttccgcaa aggcaatctg atcgccctgc
     5581 gcgaactggc actgcgccgt actgccgatc gcgttgatga gcaaatgcgc gcctggcggg
     5641 ggcatcctgg cgaagagaaa gtctggcaca cgcgcgacgc gatcctttta tgcatcgggc
     5701 ataacaccgg cagcgaaaaa ctggtccgcg cggcggcgcg gctggcgtca cggctgggaa
     5761 gcgtctggca cgcggtgtat gttgaaaccc ctgccctgca ccgcttaccg gagaaaaaac
     5821 gtcgggcaat tctcagcgcc ttacgtctgg cgcaggagct gggcgcggag acggcaacac
     5881 tttctgatcc agcggaagag aaagcggtag tgcgttatgc ccgtgaacat aatctcggca
     5941 agattattct cggtcgcccg gcctcgcgcc gctggtggcg tcgggaaacg tttgctgacc
     6001 gactggcgcg aatcgccccc gatctcgatc aggtgctggt cgcgcttgat gaaccacccg
     6061 cccgcacgat aaacaacgcg ccggatagcc gctcttttaa agacaagtgg cgggtacaga
     6121 ttcagggatg cgtggttgcc gccgcgttat gcgccgttat caccttaatt gccatgcagt
     6181 ggctgatggc gtttgatgcc gccaacctgg tgatgctgta tctgcttggc gtggtggtgg
     6241 tggcgctgtt ttatggacgc tggccttcgg tggttgccac cgttattaat gtggtgagtt
     6301 tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg caatatctgc
     6361 tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact gcgggtgtgc
     6421 gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta tatgaaatgt
     6481 cgaaagctct ggctgtgggc cgcagtccgc aggacatcgc cgccaccagc gaacaattta
     6541 ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac ggtaaattgc
     6601 agccgttaac acatccgcaa ggaatgacgc cgtgggacga tgccatcgcg cagtggagtt
     6661 ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta ccgtaccaga
     6721 ttctgccgtt aaaaagcggc gagaaaacct acggactggt ggtggtagaa ccggggaatc
     6781 tgcgtcagtt gatgatcccg gaacagcagc gcctgctgga gacatttacg ctgttagtcg
     6841 ccaatgcgct tgagcggctg acgctgaccg ccagcgaaga acaggcgcgg atggcaagcg
     6901 aacgagaaca gatccgcaac gccctgctgg cggcgctttc gcatgattta cgcacgccgc
     6961 ttacggtgct gttcggtcag gcagaaatct taacgctcga tctggcaagc gaaggatcgc
     7021 ctcacgcccg ccaggccagc gagattcgcc agcatgtgct gaacacgacc cgactggtga
     7081 ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag aaagagtggt
     7141 taacgctgga agaagtggtc ggcagcgcgc tgcaaatgct ggaaccgggt ttatcgtcgc
     7201 ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg ccgctctttg
     7261 aacgggtgct gattaatctg ctggagaacg cggtgaaata cgcgggggcg caggccgaaa
     7321 ttggtatcga tgcccacgtt gagggcgaga agctacaact ggatgtctgg gataacggcc
     7381 ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc ggaaataaag
     7441 agtcggcggt accgggtgtc gggcttgggc tggcaatttg tcgggcgatt gtggatgtac
     7501 acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt cgtgttacac
     7561 ttccccagca aactgcccct gaacttgaag aatttcatga ggatatgtga caaacgttct
     7621 gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg agggcgacgg
     7681 tatgcgcgtc tttgaggccg aaacgctgca acgcggcttg ctggaagcgg caacccgtaa
     7741 gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg agtttatccg
     7801 cgacctgcgc cagtggagcg cggtgccggt gattgtgctt tccgcacgta gcgaagagag
     7861 cgataaaatc gccgcgctgg atgccggagc ggatgattac ctgagtaagc catttggtat
     7921 tggcgaattg caggcccgtc tgcgcgtcgc tttacgtcgc cactccgcca ccgctgcgcc
     7981 cgatccgctg gtgaaatttt ccgatattac cgtcgattta gccgcccgcg tgatccatcg
     8041 cggtgaggaa gaggtgcatc tcaccccaat tgagtttcgc ctgctggcag tgttgcttaa
     8101 taatgcggga aaagtgctca cccagcgcca gctccttaac caggtgtggg ggccaaacgc
     8161 ggtcgaacac agtcattatt tgcgtattta tatggggcat ctgcgacaaa aactggagca
     8221 agatcccgcc cgcccccgcc acttcattac tgaaaccggt attgggtatc ggtttatgct
     8281 ttgaattgtt attcaataaa ttcgctttaa tagtgattaa tttattatta aacctgctcc
     8341 aaatattatt atttggcacg tttaatttct taacgcaaat ataaaacacg aaat
//
LOCUS       DAOTRK010000180         8263 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.181, whole genome shotgun sequence.
ACCESSION   DAOTRK010000180 DAOTRK010000000
VERSION     DAOTRK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.181"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..95)
                     /locus_tag="QIE41_004185"
     CDS             complement(<1..95)
                     /locus_tag="QIE41_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDS3532991.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWL"
     gene            571..1842
                     /locus_tag="QIE41_004186"
     CDS             571..1842
                     /locus_tag="QIE41_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDS3532992.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFTNITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1872..2876)
                     /gene="dppF"
                     /locus_tag="QIE41_004187"
     CDS             complement(1872..2876)
                     /gene="dppF"
                     /locus_tag="QIE41_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDS3532993.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2873..3856)
                     /gene="dppD"
                     /locus_tag="QIE41_004188"
     CDS             complement(2873..3856)
                     /gene="dppD"
                     /locus_tag="QIE41_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDS3532994.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLSDGRQSKCHYPLDDAGRPTL"
     gene            complement(3867..4769)
                     /gene="dppC"
                     /locus_tag="QIE41_004189"
     CDS             complement(3867..4769)
                     /gene="dppC"
                     /locus_tag="QIE41_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDS3532995.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4779..5798)
                     /gene="dppB"
                     /locus_tag="QIE41_004190"
     CDS             complement(4779..5798)
                     /gene="dppB"
                     /locus_tag="QIE41_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDS3532996.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5949..7556)
                     /gene="dppA"
                     /locus_tag="QIE41_004191"
     CDS             complement(5949..7556)
                     /gene="dppA"
                     /locus_tag="QIE41_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDS3532997.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(8185..>8263)
                     /locus_tag="QIE41_004192"
     ncRNA           complement(8185..>8263)
                     /ncRNA_class="other"
                     /locus_tag="QIE41_004192"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         1911 a         2248 c         2088 g         2016 t
ORIGIN      
        1 ttcagccagt tcacgatcat gctggcaaga atgccagcaa tgaccggagc cgctaaatca
       61 tgccagaagg ccatgcccag ctctgcgagc gtcatatagc cgcctgagtt gtaatgacaa
      121 cgtttcgcgg ctattcttgt attgtttgga tgcaagaatg gcccccgtgg tgttgtcagg
      181 tgcatacctg caacgtgcgg gggttttctc tctccagcaa ccaatgccac cagggattaa
      241 gcccccgcaa cattgcgcct caccggaacc tttccggctt gccgctgatt ttacgattgc
      301 tttttaccgt cagcacccgg caatcttcct cattgcgcga tgccttccag cttaatttct
      361 ttatttgcaa tagttgcagt tcatatccga gctgactcca gactggtagt gaaatgcgca
      421 attttgtaga attgcgcctc ctcgcgacct ccccagtgaa tctgttgcct cattttttac
      481 atactgcgta ttcgacttct ccacctgttg cgcaagagaa actgggttta ttcatttttg
      541 cgaggccgac ttctttctgg acaggacttt atgcagcaca acacactacc gaaacacgat
      601 cagaaattgc cgtttacacg ctacgacttc ggctgggttt tattatgcat aggcatggcg
      661 attggtgccg gaaccgtgct gatgccagta caaattggct tgaagggaat ttgggtattt
      721 attaccgcag cgatcattgc ttatcctgcc acctgggtag tgcaggacat ttatttaaaa
      781 accctttctg aaagcgattc ctgtaatgac tacaccgata ttatcagtca ttacctgggg
      841 aagaactggg gaattttcct cggggttatc tactttttga tgattatcca cgggattttt
      901 atctactctc tctccgtggt tttcgacagc gcctcgtacc tgaaaacctt cggtttaacc
      961 gatgccgatc tttcacaatc tctactttat aaagtcgcca ttttcgccgt actggtggca
     1021 attgcgtctg gtggtgaacg attactgttt aagatttccg ggccaatggt ggtggtcaaa
     1081 gtagggatta tcgtcgtgtt cggttttgcg atgatcccgc actggaattt caccaatata
     1141 accgccttcc cgcaagcctc cgtctttttc cgcgatgtcc tgcttaccat tccattttgc
     1201 ttcttttctg cagtatttat tcaggtactt aacccaatga atattgccta tcgtaaacgg
     1261 gaagcagata aagtactggc aacccggctc gcgctgcgta cccaccgaat tagttatatc
     1321 acgctcatcg cggtgatcct gttttttgcc ttttcgttta ctttctcaat tagccacgaa
     1381 gaagccgttt ctgcctttga acaaaatatc tcggcactgg cactggccgc gcaggtgatc
     1441 cctgggcata tcattcatat cacctctacg gtgctgaata tctttgccgt actgaccgca
     1501 ttctttggca tttatctcgg tttccacgag gctattaaag gcattattct caatctgtta
     1561 agccgaatta ttgataccaa gaaaattaac tcacgcatgc tgactctggc gatctgcgct
     1621 tttatcgtca ttacgttgac gatttgggtt tcgtttcgtg tatcggtgct ggtgttcttt
     1681 cagttgggaa gcccgttata tggcattgtg tcgtgcctca ttccattttt cctgatctat
     1741 aaagtcgcac aactggaaaa acttcgcggg tttaaagcct ggctgattct gctgtacggc
     1801 attttgttat gcttgtcgcc actgttgaag cttattgagt aaaccggagc gcatggcccc
     1861 ggttttgtga gttaacgctg cggattttca tcctgatcaa cagcaaaaca agctaccagt
     1921 tgaccgccgt agtcttttag ctgcggctgc aactgagtgc aggggccgaa gcgccgacga
     1981 cagcgggcgt taaacgcgca gcccggcggt ggattgagcg ggcttggcag ttcaccggtg
     2041 agcttgatgc gctcgcggcg atcgtccggg ttcaggcgcg gcgtcgcgga gagtagcgcc
     2101 tgagtgtacg gatgacgcgg gttattgaag atttggtcct tcgttccctt ctccacgcag
     2161 cggcccaggt acatcaccat cacttcatcg gcaatgtgct caaccactga caggtcgtgc
     2221 gagataaaga cataagacag ccccaactcc tgctgcaaat ccatcatcag attcagcacc
     2281 tgcgcacgca ctgaaacgtc cagcgcggaa accggttcat cggcaatcac cacatccggg
     2341 tcgagcatca gaccacgggc aatggcgata cgctgacgct gaccgccgga gaacatatgc
     2401 ggatagcggt cataatgttc ggttttcagg ccgactttcg ccatcatcga cagggctttt
     2461 tcccgacgct gttctttgct taagctggta ttaataagca gcggctcttc aagaatttgc
     2521 ccgacttttt tacgtggatt cagcgaaccg taagggttct ggaagacaat ctggattttc
     2581 tgccgacgca gcttctgcgc ctgcggatcg tgcttgagca gatcctgccc ctggtaatac
     2641 agctcaccac cggtgggcat ttcgatcatc gtcagcaacc gaccgagggt cgatttaccg
     2701 cagccagatt cacctactac tgccagcgtt ttgccacgtt caaggttaaa cgaaacgccg
     2761 tccagcgcct tcaccagacg ttccggcgcg aacatacctt tcttcaccgg ataatgtttt
     2821 ttcaggtcga tagcctgcaa cagcggttgt tgcgaggtgg cctcatgcgt actcatagtg
     2881 tcggcctccc ggcatcatca agtgggtaat ggcatttgga ctgacgcccg tcagagagca
     2941 tattcagcgc cggttcttca gcgcgacatc tgtcagtggc ataggggcag cgcgggttaa
     3001 gcaggcagcc gttcgggcgg tcatacttgc cgggaacaac acctggcaac gacgccagac
     3061 gttctttgtc ctgagcaaat tccggcagcg cacgcagcaa tgcctgagta tatggatgac
     3121 gcggcgcatg gaagatggcg tgggcatcgc ccgtttccac cacctggcct gcatacatca
     3181 cgatgatttt atgtgccgct tccgccacca gcgccaggtc atgggtaatc aacaccagcg
     3241 ccatgttctc tttctgctgt agctccagca acagttcgat gatttgcgcc tgaatggtca
     3301 cgtccagcgc agtggtcggc tcatcggcaa tcagcagttt tggtcgacag gcaatcgcca
     3361 tggcgatcat cacgcgctgg ctcatgccgc cggaaagctg atgcgggtaa acatccagac
     3421 gcgatgccgg atcgggaata ccgacctgat tcagcaggtc aatcgcccgc tgacggcggg
     3481 tacttttgtt gccgccctga tgcaccttaa tcgcttccat aatctggaaa cccacggtgt
     3541 agcacgggtt aaggctggtc atcgggtcct ggaagatcat cgccacttcg gcacccacca
     3601 ggttgcggcg ctctttttct gagatacgct gtaaatcctg accgttaaac tccagctttt
     3661 ccgccattac gcggcccgga taatcaatca gccccataat cgccagcgaa ctgaccgatt
     3721 taccagagcc ggactcaccc acaatcccga ccacttcgcc ctgttttacg ctgtagctga
     3781 tgcggtctac ggcgcggaac ggtgcgcttt cgtcgccgaa atgcaccgat aatttatcta
     3841 catttaataa cgccatctcg aacctcttac tgcttcagtt tgggatcgag cgcgtcacgc
     3901 agaccgtcac ccatcaggtt aaatgccagc accgtcagca ggatcgccag acccgggaag
     3961 gtcacgaccc accaggcgct ttgcgcgaac tgcaacacgt cggagagcat ggtgccccac
     4021 tccggcgttg gcggctgcgc ccccatgccg aggaaaccaa gagcagccat atcgagaatg
     4081 gcgttagaga aaccgagcga cgcctgaaca atcagaggcg caaggcagtt cgggaagatg
     4141 ttaataaaca tctgacgcat cgccccggca cctgccacgc gagacgcggt aacgtaatcg
     4201 cgattcacct ccaccagcac ggcggcgcgg gttaagcgca catagtgcgg caaggcaacg
     4261 aaggtcaatg ccagcgcggc gttaccaatc gacgggccga aaattgccac cagcaccagt
     4321 gccaacagca aacttggcag cgccagcatg atatcgacca cgcgcatgat gatgttatcg
     4381 accaggcctc cgaagtaacc ggcgatcagg ccgagaataa cgcccataat cagcgataac
     4441 acaactacca gacagccaac cagcagtgac aatcgcgcac cgtacatcag acgcgacagc
     4501 acatcacggc ctacgtcatc ggtgcccagc aagtgcgcca tgctgccgcc ttcctgccag
     4561 gctggcgggg cgagcagtgc atcgcggaac tgttccgccg ggttataggg tgcaatccag
     4621 ttggcaaaga tcgcgataaa cagcacgatg acgacgtaaa ccagcccgac gaccgcgcct
     4681 ttgttgcgtt taaaatagtg ccagaactcc tgtaacgggg tcatcggcac cggtgcgcta
     4741 atcactttat tttcagtaac ctgtgacatg atgtcccctt acttcttatg acgaatacgc
     4801 gggttcacca cgccgtacag cagatcgacc agcaagttga cgaggataat catcgtcgcc
     4861 accagcaata cgccgccctg cactaccgga tagtcgcggc gttgcagtgc gtcaatcaac
     4921 cagcgcccca gaccgggcca cgagaagatg gtttcggtca gaatcgcccc cgccagcaat
     4981 gttcccacct ggaggccgat aacggtcact accggcagca tcgcgttacg cagcgcatgg
     5041 acgataatca cccgcatgcg ggtcaaccct ttggcgcgcg cggtgcggat ataatcttca
     5101 cccagcactt ccagcatcga ggagcgtgtc atacgtacaa tgaccgccag cgggatagta
     5161 cccagcacaa tggcgggcag gatcatatgg gcgacggcat cgataaagtt accgtcttca
     5221 ccccagatgg cggtgtcgat tagcataaaa ccggttaacg gattggaatc atcgaggaac
     5281 accatgtcgc tcacgcgacc ggagacgggc gtcaggttcc agtgcaccga aaccagcatg
     5341 atcagcatca tgccccacca gaagataggc attgaataac ctgtcagcgc caggccaacc
     5401 gccgtgtgat cgaaaatgga accgcgttta accgcagcca gcacaccaac cggaataccg
     5461 actgccgtag caaaaatcat cgcgcagacg ccaagttcca gcgtggcctg aaagcgcggc
     5521 acgaactctt cccataccgg gatgcggctt ttcatcgaaa tgcctaaatc gccgtgcatt
     5581 acgccccaaa tgtaatggag atactgctgc cacatcggtt tatctaagcc gagttcagcc
     5641 agcagctgcg cgtgacgctc tggggagatc ccacgttcgc ccgccatgat catcaccgga
     5701 tcgcccggga tcatgtggac aaaggcaaat gtgagaaggg taataccgat aaacgtgggg
     5761 atgacgagtc ccaaacgtcg gagaataaac tgcaacataa cccggattct ctgtagtgac
     5821 ccgcagcctg gaactgcgtg tctgtattgc tcacaaatca attcccgccc aggtcgggca
     5881 gggaatgaag tattgctgcc ggatggcgct gcacgcagag gcacagcgcc accgggtatg
     5941 gcttttaatt attcgataga gacgttttcg aagtgatgtt tgcctaatgg atcaaccaca
     6001 tagcctttaa cttctttgcg taccggttca aacacggtgg agtgagcgat gatcagtgcc
     6061 ggagcctgat cgtgcatcac cacctgcgct tgcttgtaca gttcaacgcg tttgttatgg
     6121 tcgtcggtag cacgcgccgg ttgaatcaga tcttcaaacg gtttgtagca ccattttgag
     6181 tagttggagc cttgttcaga ggcggcgcag ctgaacaggg tggcgaagaa gttatccgga
     6241 tccccattat cgccagtcca gcccatcatt accgtctggt gctcgccatc tttcgcacgc
     6301 ttgaggtact caccccattc gtaggtgaca attttggcct gcacgccgac tttcgcccag
     6361 tctgcctgaa tcatctccgc catgcggcga gcgttcgggt tatacggacg ttgtaccggc
     6421 attgcccaca ggtcgatgga gaagcctttt tccaggcccg cttctttcag caaggctttc
     6481 gctttttcgg gatcgtaggt gtagtcctga acgtcgtcgt tatagcccca catggttggc
     6541 gggatcaggt ttttcgctgc cacgcccgcg ccctgataaa ccgctttgat gatcgcgtct
     6601 ttgttcaccg cgtaggtcag agcctggcga actttcacat catcgagtgg ttttttctgc
     6661 acgttatatg aaagatagcc gacgttcagc cccggcattt ccatcaggtt gatggattta
     6721 tcctgcttca tgcgagcgat atctgccggg ttcgggtacg gcatcacctg gcattcattc
     6781 ttctgcaatt tcgcgtaacg cacggaagca tcaggggtaa tagagaaaac cagcgtatcg
     6841 atctgcggtt tggtgcccca gtagccatca aacgctttgt agcggatacg ggaatctttt
     6901 tgatactgct gtaactggaa cggaccggta ccgattgggt tgaggtccag tttttccggt
     6961 gtaccggctt tcatcatcgc gtcagcatat tcttttgaca gaatagaggc gaagtccatt
     7021 gccaggtcag cgaggaacgg cgcttccggt cgagtcagca caaactgaac ggtgttgtcg
     7081 tccacctttt tcacttcgct gatcagctcc ggcaagccca tgccttcgaa gtattcgtag
     7141 ctgccgccag agactttatg gtacgggttt tgcgcgtttt tctgacgatc gaacgagaac
     7201 accacgtcat cggcgttcag ttcgcgcgtc ggtttgaatt ctttgttgtc gtgccacttc
     7261 acacctttac gcagatggaa ggtataggtt tttccgtctt cgctgacttc ccacttttca
     7321 gcgaggcccg gaatcacttc ggtggtgccg attttaaatt caaccaggcg gttataaagc
     7381 ggcacggaag aggcgtcata ggtagtaccg gaggtaaaca actgcgggtt aaacccttcc
     7441 ggagatcctt ctgagcaata aaccagagtt ttagcctgaa cacttgctgc gacggtcata
     7501 gccaccaggc tgagaccaag cttcagcatc cctgactttt tcaaggaaat acgcattatt
     7561 ctgctccaat tgtgatgttt gttgttttaa ccctttgcag tgggttgtcg ctgcctgacc
     7621 ttttttgttt tttgcccggt cgggtcaacg ttatgaggtg gggatgccgt actaattaac
     7681 atcagtgtta cagtgcggat cctccataaa atgcccctcg tgacctacaa tctgtcaaca
     7741 gaatgtgaaa acgtcaatac agcccaccgg gatttacacc aacggtgaga atccacaaac
     7801 aaagattaaa aaaacttcaa acagctattt gcagcagaga aatttgtgct actccaacat
     7861 aaccagaaca atcagcttaa taattagcaa caattggtga ttaagtttta tgcattttgt
     7921 gaaaaaaatc tgatgaaaag gtggatatct gagcgattaa taccatgaac gctaaaccgc
     7981 acagcggaaa atgccagcgc cagccataag taacgcaaaa aaagatttaa gcaaatataa
     8041 aaaaagacaa tggtttctgt cacaaatccg ttaatggata gtgagatatg gggcgcaaat
     8101 ttggcaggga aatgtgtgga gtgttgccgt ttagatcgga taagacggat acgctgtatc
     8161 ccacaatgag tgcggtgccg aatttggtcg gtgccagagg attactcgcc acttcgtggc
     8221 tcgccctgcg ggccgttgct ggcgcaacgt tctctcgctt cgc
//
LOCUS       DAOTRK010000181         8204 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.182, whole genome shotgun sequence.
ACCESSION   DAOTRK010000181 DAOTRK010000000
VERSION     DAOTRK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.182"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            354..1178
                     /gene="gadX"
                     /locus_tag="QIE41_004193"
     CDS             354..1178
                     /gene="gadX"
                     /locus_tag="QIE41_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDS3532998.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPSLLVHNCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGNFYGTDRSTEGIRL"
     gene            1547..2275
                     /gene="gadW"
                     /locus_tag="QIE41_004194"
     CDS             1547..2275
                     /gene="gadW"
                     /locus_tag="QIE41_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDS3532999.1"
                     /translation="MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2638..5751)
                     /gene="mdtF"
                     /locus_tag="QIE41_004195"
     CDS             complement(2638..5751)
                     /gene="mdtF"
                     /locus_tag="QIE41_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HDS3533000.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5776..6933)
                     /gene="mdtE"
                     /locus_tag="QIE41_004196"
     CDS             complement(5776..6933)
                     /gene="mdtE"
                     /locus_tag="QIE41_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDS3533001.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGTLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6993..7271
                     /locus_tag="QIE41_004197"
     CDS             6993..7271
                     /locus_tag="QIE41_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533002.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLTSPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7272..7799)
                     /gene="gadE"
                     /locus_tag="QIE41_004198"
     CDS             complement(7272..7799)
                     /gene="gadE"
                     /locus_tag="QIE41_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDS3533003.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         2131 a         1892 c         1926 g         2255 t
ORIGIN      
        1 taacgttgta aaaaccgaat gcccaacctt taaaaaacag ttgggcattc ggttgcttat
       61 taatagcaat aatatattgg ctgtctattc atcgtgttga taagatatat gttatatgaa
      121 tgtttattca atgtaacgaa tattttaatg tcactacaag ggattatttg cttactatta
      181 atttcactgc gttcttaagc acgccttttg tattataaat ccagtcatcc tgcccgtaga
      241 ttatcatcct gttttcccgc ttcgtttaaa tttatttatc aatcaatttg acttaagagg
      301 gcggcgtgct acattaataa acagtaatat gtttatgtaa tattaagcca actatgcaat
      361 cactacatgg gaattgtcta attgcgtacg caagacataa atatattctc accatggtta
      421 atggtgaata tcgctatttt aatggcggtg atctggtttt tgcggatgca agccaaattc
      481 aagtggataa gtgtgttgag aatttcgtat tagtttcaag agacacgctt tcattatttc
      541 tcccgatgct caaggaggag gcattaaaac ttcatgcaca taaaaaagtt ccttcattac
      601 tcgttcataa ctgtaccaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga
      661 ataagaatct tcgctatgca gaaatgctac gtaaaagggc attaatcttt gcgttgttat
      721 ctgtttttct tgaggatacg cagtttatac cgttacttct gaacgtgttg caaccgaata
      781 tgcgaacacg agtttgtacg gtcatcaata ataatatcgc ccatgagtgg acattagccc
      841 gaatcgctag cgaattgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag
      901 gtacgtcata ttcacagttg cttactgagt gtaggatgca gcgtgcttta caacttattg
      961 ttatatatgg tgtttcaatt aagcgagttg cagtatcctg tggatatcac agcgtgtcgt
     1021 atttcattta cgtttttcga aattattatg ggatgacgcc cacagagtat caggagcgat
     1081 cggcgcagga attaccgaac tgtggctcgg cggcaagtat ggctgcgcaa gggaattttt
     1141 acggcactga ccgttctacg gaaggaataa gattatagag ttttactcag acataaaaaa
     1201 aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta
     1261 taacactccc tgttggcacg ggaaactttg tgctctcagt aagttaaata taacttttac
     1321 tggaaataag atcagccatt tttttataaa cataagctat acgctgtgcg aaaatatatt
     1381 cgtgttgcat ttacttatta tcaattaact gttatgcaaa actactttgt ggataaattt
     1441 tggtcctacc aaatctggca gtttttgcgc taagaaacag tctggcatca tttcattagt
     1501 atactgaaat tgaaataatc gcagtaatga aatataaggg atagtcatga ctcatgtctg
     1561 ctcggtgatc ctcgttcgtc gttcattcga tatttatcat gaacagcaaa aaatatcgtt
     1621 gcacaacgag agtatcctgt tactggataa aaatttggca gacgattttg cgttttgttc
     1681 accggatacg cgacgactgg atatcgatga gctgacagtt tgccattact tacaaaatat
     1741 tcgtcagata ccacgcaatt tagggttaca tagcaaagac cgtctgttaa ttaaccagtc
     1801 accccccatg ccgctggtga cggcgatttt tgatagtttc aatgactccg gggtaaattc
     1861 accgatactg agcaaaatgc tctatctttc ctgtttatcg atgttttctc ataagaaaga
     1921 actgatcccc ttacttttca atagtatcag tactgtttca ggaaaagttg aacgccttat
     1981 tagctttgat atcgccaaac gttggtatct gcgcgatatc gcggaaagaa tgtataccag
     2041 cgagagtcta atcaaaaaaa agttgcagga tgaaaatacc tgtttcagta aaatattact
     2101 cgcctccagg atgtcgatgg ccagacgatt actcgagtta cgtcaaattc ctctgcatac
     2161 tattgcggaa aaatgtggct atagcagtac atcgtacttt ataaacacat ttcgacaata
     2221 ttatggtgta acgccacatc agtttgcgca acattcgcca ggtacctttt cctgacatat
     2281 tttgcatttg aatattggtc aggatctcac acctgcttca tgtgaaactc ttccctgatg
     2341 atttctgccg ggctaccggc tagttctctt tcgcagtttg ccaggcggta taggtatttt
     2401 caggaggaaa agactcggca tgtttgggat tattaagctg acaattcata cgattacggg
     2461 aatgtgggtc agtattgtgt tgtttaaatt gatgacgaat ggctggagtg gtttctactt
     2521 tcagtgctgt gtactaagcc tggtattcct gacggttagc tggttgttga gtggtgaatg
     2581 gttagcggga aagagtaagg ctgaaccctc acgttcaacc ttactctcat ttacacgtta
     2641 cgctttttta aagcgggcaa agagatgttc cactacaacg aaaaagaccg gaacgaagta
     2701 aattgccagc actgttgcgg caaacatccc gcccatcacg ccggtaccta ccgcgttttg
     2761 cgcgccagaa ccggcaccat gactgataac cagcggcagc acgccgagaa taaaggccag
     2821 agaggtcatc aggattgggc gtaaacgcat ccgcgccgct tcgatgattg cctctatcgg
     2881 cgttttccct tctttctgca tcatctcaac ggcaaattcg acaatcagga tggcgttctt
     2941 ggcggaaagc ccgatggtgg tcagcaaacc aacctggaag tagacgtcat tacttaagcc
     3001 gcgtagatcg gtggccagta atgcgccaac gacgcctaac ggtacaacca acatcaccga
     3061 gaacggaatt gaccagctct cataaagtgc ggcgagggcg aggaacacca cgaccagtga
     3121 aatggcatac agcgcgggag cctgatttga ggataacgct tcctgatacg acagtccggt
     3181 ccatgagtag ccgacgcctg ccggaagttt agcgaccagg tctgccataa atttcatggc
     3241 gtcaccggta cttttcccgg ccgccgcttc acctaaaatc tccattgacg ggatgccgtt
     3301 gtagcgttcc agtcgcggtg aaccataggt ccattcggta gacgaatagg ccgaaagcgg
     3361 tgccatcgtg ccagaggcgt tgcgtacata ccattggttg atgttatccg gcaacatacg
     3421 gaacggcgtg cctgcctgga catacacttt tttcacccgc ccctggttga ggaagtcgtt
     3481 cacgtagctg ctgccgaagg cggtggaaat tgtctggttg atatcagaca gcgccacacc
     3541 catagcctcg gctttcgcgg cgttgacgtt cactttgaac atcggcgtat cttccaggcc
     3601 gttcgggcgt acaccggtga cttgattcgg tgattgcgct gccagtgata acagctcgtt
     3661 tcgcgcctgg gttagttttt cgtgccccag gttaccgttg tccagcagtt ccatatcaaa
     3721 acctgacgcg gtacccagtt cagccaccgc gggtaagttg aacgggaaga cgacggcttt
     3781 attgatactg cttaacgcaa tcattgcccg ctgaatgatc gcggtaaccg agttttcctc
     3841 accgacacgt tcagaccacg gcttgagact gataaacgcc aggccgttgt tttgcccctg
     3901 gccgctgaag ccaaagccgc caacggtaaa taccgactga acattatctt tctctttagt
     3961 cagataataa tccgtcacct gttgcagcac tttcgtggtg ttaaccatgg tggcaccgga
     4021 aggtaactgc gcggtggtca taaatacccc ctgatcctct tctggtaaga aagaggtcgg
     4081 cgtgcgcagg aacagcaccg ccatcccggc gcaaatcagc aggtagacca ccatgtagcg
     4141 accggtacaa cgcaacagcg agcgggtact gtcggtatag tgctgagttg atttttcaaa
     4201 cagcgtgttg aagcgtgcga acagggcgtt aggtttgtga ccgccttccg gcgcggcttt
     4261 cagaatggtg gcgcacaggg caggggtcag gctcattgcc acaaatactg aaagcagcat
     4321 ggaggagatc agcgtgatgg agaactggcg gtagatctcc ccggttgcac cgctcataaa
     4381 ggccatcggc ataaacactg cggaaagaac aacggcaatc ccgaccagcg cacgttggat
     4441 ctgccccatc gatttatgcg tcgcttcctt cggcggtagt ttatcttccg caatgacacg
     4501 ctcgacgttc tccaccacca cgatggcgtc atccaccagt aaccctatcg ccagcaccat
     4561 cccgaacata gttaacgtgt tgatggtgaa accgaccgcc gacaagatcg caaacgtccc
     4621 gagaataacc accggtacgg caatcgtcgg gatgattgtg gcacggaaat tctgcaaaaa
     4681 cagatacatg accaggaaga cgaggatgat agcctcaacc agtgttttaa aaacttcctg
     4741 aatagaaatt tcgataaacg gcgtggtgtc gtaaggataa accgtcttca gacttgcggg
     4801 gaaataggct gataagcggt tcagctcctc tttgactgcc cgcgaggtat ccagggcgtt
     4861 tgctccggca gccagtttga tggcgatccc ggcagcaggt ttgccgttat agcgcgccac
     4921 ggtggaatag tcttccgctc caagttcgac gcgggcgaca tcacgcagca gcacttgcga
     4981 accatcttgc tgaactttca acaggatttt gccaaattct tccggcgttt gcagacgcgt
     5041 ctgcacaatg atcgaggcgt ttagttgctg gtctgccgcc tgtggcatgc cacccagttg
     5101 accaccagaa atctggttgt tctgcacctt aatctgggaa ataacatcgg aaggtaccag
     5161 attgtattta ttgagttttt gcgggtccag ccagatacgc atggcgtact cggaaccaaa
     5221 gagctgtacg ctaccaacgc ccgcggtacg gcttagcggg tctttgatat tagacgctac
     5281 atagtccgcg atatcgtact ggttgaggct gccgttatca gaaataaacg ccgctaccat
     5341 caggatatta ctgctcgact tatcgacgct aatcccctgc tgctgcaccg cttcaggtaa
     5401 tgaaggcata gcgagctgca gtttattttg cacctgaacc tgtgcgatat caggcgatgt
     5461 cccagtctcg aaggtcagag tgatagaggc attgcccgcc gcatcactgg ttgaagacat
     5521 gtacatcagg ccatcaagcc cattcatatt ttgctcaatc acctgagtga ccgagtcttc
     5581 taccgtttgc gcatcggcac ctggataggt tgcgctgacg gttatggtcg gtggcgcaat
     5641 ctgcggatac tgcgcaaccg gtaagttcat gatcgccaga ccgcctgcaa gcatcataat
     5701 aatggcaagt acccaggcaa aaaccgggcg atcaataaaa tagttagcca tgaaagtccc
     5761 cttaagcctg caacgttatt gtttcgattc ggtgctggcg ttttcctggc tggaggaaat
     5821 tgctcgtgct ttgatacccg gacgaatgcg ttgcaaaccg gaaacgatca cccgatcgcc
     5881 agcctgcaag ccagaggtga caacccactg gtcgccgatg gctttgctgg cttcgatttc
     5941 gcgtagctgc acgacatcgt ctttatccag aatgagcgcc gttgctttac cctgggcgtt
     6001 gtgggtgacg ccttcctgcg gcaccagtaa tacattctgg cggctacctt catccactaa
     6061 tgccgtaacg tacatgccag gcagcaagtc accatttggg ttggggaaaa tcgcccgtaa
     6121 cgtcacggag cccgtggttt catccaccgt cgggtcggag aatttcagcg tgccggtctg
     6181 gctgtaacgt ttaccatttt ccagattgag ctgtactggc gtactgccct gaacctgttt
     6241 gatttgccca ctggcgacct cttctttcat acgcaagaaa tcctgcaccg actgcgtgag
     6301 atcgacataa atcgggtcca gacgttgtac ggtaaccagc gaatctgcct gattagcggt
     6361 aacgagtgcg ccgacggtca ccgacgattt cccgctgacg cccgtaatcg gcgaggtgac
     6421 attcgcgtat tgcaaattga tcgtcgcctg ttcaacagcc gctttggcga cggtgacatt
     6481 ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa tcctgacggc taacgtagtt
     6541 ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg gcattgctgg cggtagagag
     6601 cgctttcgcc agcgtgcctt tggcggagtt cagctcggcc tgtaaaggtg caggatcaat
     6661 ctgatacagc gaatcgccct ggttcacttt atcgccttcg ataaagttgc gtttaatgat
     6721 aataccgcca acctggggac gtatctcggc aacttcataa ggaacggttc taccgggcaa
     6781 ttcgctcaat acattgaccg aaccggggga gagtgtgacg acaccgacct caggcgtcat
     6841 ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg agcatcgcgc cgcagaataa
     6901 caacggtatt aacagctttc ttcttctgtt cattttagtc cctgaaaatt cttgagaaat
     6961 aacggttctt taaattgcgc gcgaggagag atatgaggga tttacagacc agcggcatag
     7021 tggggttgtc agccagtaag gtgggataca ggcatagtga tcgacatggt gaggtcaacg
     7081 acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag cattcgggca acgaagggtt
     7141 ctactggtgg atacacatac caggggaata aagtcgtttt aacctctcct ttagtaatca
     7201 ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc cagccatcaa tttcagttgc
     7261 ttatgtcctg actaaaaata agatgtgata cccagggtga cgatgtcgct catacgtctt
     7321 agtttcagct tgtacataat gttgaatttc tgaatcttta atgtttgttg cgaaatattg
     7381 agcgtcgacg tgatattgct ttcattttta tttttcagaa tcagttgcat aattttgaac
     7441 tgattatgtg aaaaacataa ttgtgaattc ttatggggca agtgtttacc ataagtaatc
     7501 tcaagaagat ttttgatact ttctttgcgg ctgacaaaat aaataggcgc tttagctttt
     7561 agtttactga tgtgataagg tgccaaaaca ataattctct cggcatctaa tttctccaga
     7621 aatttaatcg cttcttcatc aaggatatga ttgtgatagg tatcaatgat aaccttgaag
     7681 ggtttattgc ctactttttt gatctctgac agggaattga tttttatcat ttcgtgatta
     7741 tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt tcgtcatgag aaaaatcata
     7801 acttgctcct tagccgttat cgtttcgaac atgtcatcct tttcattatt tacatccttg
     7861 tccgaatcgt tgttcaatat agtaaacgcc tataagaact ttgcccttcg tgaactaata
     7921 cgtctattaa cactaatcaa tgcttcagat ttatccatca cacactccat atattgatcc
     7981 taagtaaaat tcctggttgt tatcagtttg taaacggcaa acaaaaactt aacgtttacg
     8041 gattactgcc caagaataag ttatcaataa ccagtttgtg atctctgaag aatattacta
     8101 aagttaaaat gtaatccgat ttaaatatcg agtctccttg tttcgactta agctggcaat
     8161 tggattgcca gctttctttt aacgcattga aattacctga gtaa
//
LOCUS       DAOTRK010000182         8160 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.183, whole genome shotgun sequence.
ACCESSION   DAOTRK010000182 DAOTRK010000000
VERSION     DAOTRK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.183"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..914)
                     /locus_tag="QIE41_004199"
     CDS             complement(<1..914)
                     /locus_tag="QIE41_004199"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDS3533004.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVD"
     gene            complement(1276..4059)
                     /locus_tag="QIE41_004200"
     CDS             complement(1276..4059)
                     /locus_tag="QIE41_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS3533005.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLQANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVR
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARANNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPLAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(4116..6488)
                     /locus_tag="QIE41_004201"
     CDS             complement(4116..6488)
                     /locus_tag="QIE41_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDS3533006.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTAVS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KYAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDERLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(6526..>8160)
                     /locus_tag="QIE41_004202"
     CDS             complement(6526..>8160)
                     /locus_tag="QIE41_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDS3533007.1"
                     /translation="LLKPFWLRKNNKTSVLLIIIILAMILGVVKIQVWLNDWNNDFFN
                     ALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIRWREWLTDYYLNRWFADK
                     NYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAG
                     TLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLV
                     QHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLP
                     YFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQL
                     TEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTL
                     LKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICKALPLPVDDKSLSEILHQ
                     VGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRL
                     LRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         2101 a         1898 c         1848 g         2313 t
ORIGIN      
        1 tagtcaacgt tgaacaccag ttcctgcggc agcgcttctt catcacgcca gataacccag
       61 ccgcagccca gcggagccag accgaattta tggcctgaag cactgatcga tttcacacgc
      121 ggcaggcgga agtcccagac gatatccggg gcgacgaacg gtgccaggaa gccaccgctg
      181 gcggcgtcga tgtgcatgtc gatgtcgata ccggtgtcgg cctggaattt atccagcgca
      241 tcgtgcagcg gctgcgggaa ctcatagtta ccggtgtagg tcacgccgaa agtcggcacc
      301 acgccgatgg tgttttcgtc gcaggcttca atcatgcgtt tcgggtccat aaacaactga
      361 ccggggcgca tagggatctc acgcagctcc acatcccagt agcgggcgaa tttatgccag
      421 cagatttgca ccggaccgca caccaggttt ggtttatccg ttggtttgcc tgcagcttcc
      481 atacgcttgc gccaacgcca tttcatcgcc atcccgccga gcatacaggc ctcggaagaa
      541 ccaatggtgt tggtgccaac ggcctgacca tttttcggcg caggcgcatg ccacagatcg
      601 gcaaccatgt ttacgcaacg caggtcgatt gcagcggatt gcggatattc ttctttgtcg
      661 atccagtttt tgttaatgga taaatccatc aacttatgga cattttcgtc gtcccaggtc
      721 tggcagaaag tggccaggtt ctgacgagcg ttgccatcaa gatataattc gtcattgata
      781 atctggaatg cgacatcatc gcgcatttcg tgcagcggaa atcgttttga ttctgcgata
      841 gtggaaatag acttcgcacc aaaacgtgaa tcgagtagtt ccgaccttaa atccgttact
      901 tgcttcttat ccattttaaa ctccttaaaa tgattggatc gcattaaaaa agtaggattt
      961 atcgataaag taagcaagtg caaaggactc gggtttaaat aacaaaatcc taatgttatt
     1021 tatcgtgaaa tattacgaga ataatgtttt ttcattaaaa aacaatgcaa tagagaattt
     1081 tttggtggtg gtaaagtgtt ttatgttgtt atttttatgc gctttctacg cctcctgaat
     1141 tatcacgtaa aaatcagacc ttaaaatatc actattagta cttggttatt attttgaacg
     1201 catttataaa attattacat aaaaatagcg aatattgcta aaatccccgc caacgaggtg
     1261 ttgacggggc tgttattatt ttggcaacaa tactccggta taggtattca ccggatgaga
     1321 aagatattgt ttaacggcag tgttaacatt ctctaccgtc atttgtttca acaattgctc
     1381 ctgctcagtc catgctgcag gatcgtcata ttgaataaga ctatttacaa tggtgttcgc
     1441 taattgttga acgctacgct gttggatatc gaggctgcgc tgaacgtttt gctggtattc
     1501 gttcagttct tgctcactga tccctttagc cagacgctta accatcactt cattcgctaa
     1561 cgttaacagt tcatcatgtc gttccggttg acaagtaaaa gccagcaaat gactgatatc
     1621 tttggcctga ggatcaaccg agaggcgaga agaaacgctg tatgccccag aggcctgttc
     1681 acgaatatta acgcgtagat cttttgccag tgcgacgtta aaagcatcga gcgccatacg
     1741 cgtctccaga tttacaggtg tccgggaatc ataacgcttc cactgtgaaa cctgtgccaa
     1801 aggttcattt tgttctttta cagtaaccga tgcgttgtcc gtcgcgcgag ttaatggttt
     1861 acctgcggct aatggcgaat cagagtgttt gattgatcct aagtaacgcg taattaacgc
     1921 cacgagtttg tcttctgcga cattaccgac aatgacaaac gtgatatccg ctggagatga
     1981 aaacaactgg cgatcggcag ccagcgcatc tgcggcagta aactgtgcaa tctgattttc
     2041 ttgcagtaat ttcgtgcggt catcagcata gcgcgtctca tacatctgct gggcgaattt
     2101 ctccgctgga cgctggtcga gcgttttcaa cgcctgaatt tgagcatttt gtagcgatgc
     2161 ccaaatatta tcgttaatcg tgctgtgggt gattcgctgg ttaatcaact ggaaaccagg
     2221 ttcagggtta ttagcccgcg cgctaacaga gagcaatgta ttcatgccac tgactttact
     2281 gctcatggtt accgagtttt ccgcactcca gcgtttcagg ctggaagagg agagttcacc
     2341 aacgccgctt ccgcttactg ctttatttgc cagcgcgata agtgattttt gctgcgcagg
     2401 gaaacttaaa tcgcctttat tagatacggc agtaatttgt agcttttgct cttcacccgc
     2461 agattttgcc agaataaccc tggcaccatt ggaaagtgtt aatgatgtca gattctcagc
     2521 cagcgtttct ttgctgctaa tttccgcctg tgggtcagcg tctactgtta acgataaatt
     2581 tctgcctggg aagatgtaag ccgccagctt tttgttggcg tactcttttt ccagcgccag
     2641 gatagccgca ggagacaatg cttttttggc agcaacctca ttgtttacca tttgctccca
     2701 aaatgcatcc tggttctttc ttaactgctg ccatttttcc gccagacttt gcacggtaat
     2761 ttgctgccac agacgtttcg aaagttgata tgtctcttcc ggcgacaaga acggcgtatt
     2821 atttaatgag ctggatgcca ggcgactggt cagcatacgt aaatcacgtt cagcttgctg
     2881 atcaaccgca tttttcagcc aggtgagacg ggtagatttg acatcatcga gttcttcagc
     2941 agaaaaacca tgctgatcaa tagttgccaa ctctgccatt aatgcattcg cagcatcctg
     3001 catattatcg tctcgtgcat ttacacggaa aaacagcgac tgataatcgg gtgcaattct
     3061 aacgctgcgc gcagtgccgc cagaaatagt cttcaactcg cccgactgta tgcgttcctg
     3121 cagacgctga ttgaacagct gaaccaacat gctccattca gcttgttcga taaagctttg
     3181 ctcatcgctc acttgcacca ttggcaggcg ataatagagt gcgatgccgt tcacccggtt
     3241 ttctttatca ttaatgatat taaagcgcag gtggttttcg gcttttgtcg gccagacgcg
     3301 attttcagct gctttgttag ccggaagctt acttaaatta tccttaatca gcgccagcgc
     3361 ttctttactg tcaatatcgc cgaccacgat aaaggtcata ttatttggtt gataccagcg
     3421 ttgataaaat tggcgcaatt gtgccggtgt gaccgtagcg acagtatcca tcaggccgat
     3481 aggttcacgg tctaaattac gggtattagc ctgcaggaaa gggcggcgcg cctgagaggt
     3541 gcgccatttc gcatcctgat gggcacgcca ttcctcagta attacgccgc gttcagcgtc
     3601 tacttcgagt ttttcaaagg ttgcggcatt actccattca ctgaagattg ccatcacttg
     3661 ttgcagattt tgtttctgcg tagtcggcaa acttacctga tacaccgttt cgtcatagct
     3721 ggtataggca ttaacatcgc gaccaaagcg caggcccatt gactcaaatg tttcgatgac
     3781 tttattaccc ggccatgttt ttgtgccgtt aaacatcata tgttctacaa aatgagccac
     3841 gccgcgctca ttgtcttctt cctgcaatga accggtatga atttgcagcc ataaatttac
     3901 ctgatccttt ggttgagcat gcggataaat catatatcgc aagccattgt ccagttgccc
     3961 ggtaattaac ttttcatcct gcggtaaggc ggcggcaatc agtcgcccgg ggagcaacag
     4021 agttgccact aacgtcagta agaaacagag gtttctcata attatctcca tgcgaaaacc
     4081 gggcgaattt acccggtaaa gtaaaatccg aactattaaa atttcatgct gacatcaagc
     4141 cagaaagtac ggccactggc gtaggtcgcc acgccggtat tcgttgtatc aacagcggtt
     4201 ttgctatcca gtacattgag aatatcggca ctgatcgtca ggttttgttg tttcagaaat
     4261 tgtggcgtcc aggacaagcg ggtgtcccag gtcaggctgc tatccagtct ctcgtcaaca
     4321 tactgcttgt aatctgaata ttcgctgatg tattgcgcat tcgtcttacc caggataatg
     4381 cgagctttac gcgcttcttg ccaggtcaac gtattcgccc acaccaaccc gctcggttga
     4441 tgcgtgaaat ccatgtttaa ggagatcttt agtggattat taaaatccgc cactggaacg
     4501 ctatcgtaag agaccagatt accgttatat accacccggt tatcaccggt attgctctcc
     4561 tcataaccat tgtttaacga caagttgccc ttgctcttga tatagctaaa gacaatttgc
     4621 gggttaatat ctacctggct gatatgcagg ggttcggcca gttcaaaact gaggttgaac
     4681 gaatgcgttt tggttttgcc gtcgttgtta tattcagtaa tggtggtttt agtcgcgctg
     4741 tcggtacgac tgcttttact gatttgatca tgcgcttcac gataaacata gtttgcacgc
     4801 gcaataacgt tctttccgat tttctgctgc aaccccattg ccagttcatc gttataaggt
     4861 gttttcaaat cctgataacg cgtcagggtt ttattgcctg ataccgattc cgtccagcta
     4921 ttgcggatat cacgtaatcc catatcaaga atattcccgc catagtaacg gttataaccg
     4981 gcagtaatca ttgaggtttg atcagcaaaa atatcccatt ccgtcataaa tcgcggggag
     5041 atattgtggt ttgacagata gttgtcatag tcatatcgca caccgggcat taacgacaca
     5101 ttccgccagc taatgcgatc cgccatatac agcgtgtagt tgtcaattcc cagactgcct
     5161 ttacctttat ggtaaatggt atggttagtt ttctttcctg cagcattaat cacataggat
     5221 tcagactggt tatggcgttc agtccatgca tcggaataga tgtattccgc gccgaagtag
     5281 ggttgatgcg aaacattacc cacggcgtat ttttgccagt ccaggcgtgt tttgaaggtg
     5341 taattatcta cagcctggga aatgtgtcct aatccgccac gggtgcaacg ccctgaaata
     5401 tcaccatatg tacatgaaag ttcggtgtac catatgtcat gatcgtgacg ggtataatca
     5461 ctaatatgat cccaaccaac ggtagtacgc agtttggccc atgcgagctg cgtatccata
     5521 tcccatgcca gaccatagga tttattaccc atttcgcgat cagactccgg gaaggtgctg
     5581 gtattataat cacggctgga gccggtatat tttaaggtta aatcgtgggt aaagcggtca
     5641 ctggcaaacc aggtaaattt gctcactgcg gtatcgataa cgtttttata ctgtgcccga
     5701 ccggcgacaa tgccgtcatt cgaaacataa tccgcgcggg tgatatcaga ctggcggcgc
     5761 gataaaccgg cggtaacgcc gaagttatca gcgagttcct gattaaatga caaagtataa
     5821 aagttctttt taaaatctgg ggagtaataa gtacttcctg aagagccttg attaaatgcg
     5881 ctcttgttat tctcatcgat atgcgatgtt aaccagtccg aacgcgtagt gcggtaaccc
     5941 agtttcacgc tgctatcatc agcgttgaag cgtttgatct ttgcatcaat taccccgcca
     6001 ttgaagcgac caaattcaac cggcacaaaa ctgtcataaa gcgtcacatt gtccagtaag
     6061 ctgacatcaa gataataccc ctgtgacatc ccgctaatat tggttgcact actggcatcg
     6121 gactcattcg ctgggttcag gttattagtt gcactaatac cgtcaatcaa ataggcattc
     6181 tggtagggcg acgcaccgtg aatagagatt ttctcagggc gaatatctcc ctggttcaat
     6241 gaggtacttt gcgttgaatc catgcgtacc gcagggttgg ttctcagcag atcgctgata
     6301 ttgccattcc cggtcggtaa acgctcgatg ctttcactgg tgtaatgcgt gttgccgttt
     6361 acgggtacag ggacaggagc atagaccgtc atctcttcaa aaacatgcat gtacatgttt
     6421 ttgtcatcga cggcctgcgc cacatcagcg ccacataaaa tgacgccagg aataagaact
     6481 cgcttcattt atttttatct ccgtacagca gggcttttat tgtttttata aaaccgcgct
     6541 aatgtcacaa atatcatcag ccaggttcca gacaccgggt tgatgtgtaa ccataatgac
     6601 gccgcttgtg ggtagttttt cacgcactaa acgcagtaag cggatagcct cttgttcctc
     6661 aagatgagag gtagtttcgt caagaaatat ccatttcgga cgtcgtaaaa ttaatcgtgc
     6721 cagggcgata cgttgttttt cgccgctgga aagaatatct ccccagcgat cgtggtcatg
     6781 aatacgcgca gccaatttcc ccagaccaac ctgatgcagt atttcgctca acgatttatc
     6841 gtctacgggc aggggaagtg ctttacaaat aatctctttc agtaagccgg ttttgattaa
     6901 cggtgtttgt gacacatacc aactgtcagc aggagaagaa atatcacctt tgaaccacgg
     6961 ccagcagtgg gataatgttt taagcagtgt ggtttttccc gcaccagagt aacctttcag
     7021 taatagccat ttgcctggcg aaacatgaaa gttcaggttc tctaatatga tcttattatc
     7081 aggagtacga atactcgcgt cagccacttg taccgcatgt tggcaatttt taggcttatt
     7141 cgtagggcgc tgttcggtga gttgatggaa ctcatacaaa cgatcgataa ccgcagccag
     7201 ttcagcaagt tcgtcatatt tataaataaa ccagcttaaa ttgttcgata ccagcataaa
     7261 tgcctggcgc gatttcatca gtccgcccag attgatctgt ccgctaataa actgtggcaa
     7321 taacagaaag tagggaagaa cgctaagcga gcgcgaataa atattctgcc agtaatcaag
     7381 ccagcgctga cgattcatta aacgatgcca gttctcttta atggtatgaa aattgtcgct
     7441 caactcctgg cgttgtagag actcagcatt gcttagcgca atcagttcag cctgcttatt
     7501 atgctgcaca agattagttc gaaatgtcgc ttcgctacgt tgcttttcca cattaagcgg
     7561 acgaatacgt ttaccaacct tatgagtaaa taaagttcca ccgatcacaa tgagcacaac
     7621 ggtatagacc atatacccct ggatattcca ttctgttcca ccaacagtga atgagagcgt
     7681 acccgcgctt tgccaaagaa taacggtaaa ggtgatcagc atgctaagtg actggatgaa
     7741 gccaaatgaa agactgagcg ttttgctgat cagtaagaga atgtcttcag cgatacgttg
     7801 gtcagggtta tctgtatttt tatgctcgcc gtagatctgc gtgaagtagt agtttttatc
     7861 tgcaaaccac cgattaaggt agtaatctgt taaccattcg cgccagcgaa tagtcagcag
     7921 tttaattaac catgttttgt ttacggatat taatacaaag atccccagca gggcagggaa
     7981 ccaaaggaca agctgccaaa gcttatcggt ttctttctgg cttagcgcat tgaaaaaatc
     8041 attattccag tcgtttaacc agacctgaat tttaaccacg ccaaggatca tggcgaggat
     8101 aataatgatt aacagcactg aagttttatt attcttacgc agccaaaagg gcttcagcag
//
LOCUS       DAOTRK010000183         8145 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.184, whole genome shotgun sequence.
ACCESSION   DAOTRK010000183 DAOTRK010000000
VERSION     DAOTRK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.184"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            67..924
                     /gene="focA"
                     /locus_tag="QIE41_004203"
     CDS             67..924
                     /gene="focA"
                     /locus_tag="QIE41_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDS3533008.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            979..3261
                     /gene="pflB"
                     /locus_tag="QIE41_004204"
     CDS             979..3261
                     /gene="pflB"
                     /locus_tag="QIE41_004204"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDS3533009.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3453..4193
                     /gene="pflA"
                     /locus_tag="QIE41_004205"
     CDS             3453..4193
                     /gene="pflA"
                     /locus_tag="QIE41_004205"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDS3533010.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            4292..4402
                     /locus_tag="QIE41_004206"
                     /pseudo
     CDS             4292..4402
                     /locus_tag="QIE41_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4403..5833)
                     /locus_tag="QIE41_004207"
     CDS             complement(4403..5833)
                     /locus_tag="QIE41_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDS3533011.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(6043..7191)
                     /locus_tag="QIE41_004208"
     CDS             complement(6043..7191)
                     /locus_tag="QIE41_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3533012.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            7506..8132
                     /locus_tag="QIE41_004209"
     CDS             7506..8132
                     /locus_tag="QIE41_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDS3533013.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
BASE COUNT         2242 a         2057 c         1906 g         1940 t
ORIGIN      
        1 tatcaatttc tcatctataa tgctttgtta gtatctcgtc gccgacttaa taaagagaga
       61 gttagtgtga aagctgacaa cccttttgat cttttacttc ctgcagcaat ggccaaagtg
      121 gccgaagagg caggtgtcta taaagcaacg aaacatccgc ttaagacttt ctatctggcg
      181 attaccgccg gtgttttcat ctcaatcgca ttcgtcttct atatcacagc aaccactggc
      241 acaggaacaa tgcccttcgg catggcaaaa ctggttggcg gcatttgctt ctctctgggg
      301 ctgattcttt gtgttgtctg cggagccgat ctctttactt ccacagtgtt gattgttgtt
      361 gctaaggcga gtgggcgaat cacctggggt cagttggcga aaaactggct aaatgtctat
      421 tttggcaacc tggtcggcgc actgctgttt gtacttttaa tgtggctttc cggcgagtat
      481 atgaccgcga atggtcaatg gggactaaac gtcctacaaa ccgccgacca caaagtgcac
      541 catactttta ttgaggccgt ctgccttggt atcctggcaa acctgatggt atgtctggca
      601 gtatggatga gttattctgg ccgcagcctg atggataaag cgttcattat ggtgctgccg
      661 gtcgcgatgt ttgttgccag cggttttgag cacagtatcg caaacatgtt tatgatcccg
      721 atgggtattg taatccgcga cttcgcatct ccggaattct ggaccgctgt cggttctgca
      781 ccggaaaatt tttctcacct gaccgtgatg aacttcatca ctgataacct gattccggtt
      841 acgatcggta atattatcgg cggtggtttg ttggttgggt tgacatactg ggtcatttac
      901 ctgcgtgaaa acgatcacca ttaatggttg tcgaagtacg cagtaaataa aaaatccact
      961 taagaaggta ggtgttacat gtccgagctt aatgaaaagt tagccacagc ctgggaaggt
     1021 tttaccaaag gtgactggca gaatgaagta aacgtccgtg acttcattca gaaaaactac
     1081 actccgtacg agggtgacga gtccttcctg gctggcgcta ctgaagcgac caccaccctg
     1141 tgggacaaag taatggaagg cgttaaactg gaaaaccgca ctcacgcgcc agttgacttt
     1201 gacaccgctg ttgcttccac catcacctct cacgacgctg gctacatcaa caaagcgttg
     1261 gaaaaagttg ttggtctgca gactgaagct ccgctgaaac gtgctcttat cccgttcggt
     1321 ggtatcaaaa tgatcgaagg ttcctgcaaa gcgtacaacc gcgaactgga cccgatgatc
     1381 aaaaaaatct tcactgaata ccgtaaaact cacaaccagg gcgtgttcga cgtttacact
     1441 ccggacatcc tgcgttgccg taaatccggt gttctgaccg gtctgccaga tgcttatggc
     1501 cgtggtcgta tcatcggtga ctaccgtcgc gttgcgctgt acggtatcga ctacctgatg
     1561 aaagataaac ttgcacagtt cacctctctg caggctgatc tggaaaacgg cgtaaacctg
     1621 gaacagacta tccgtctgcg cgaagaaatc gctgaacagc accgcgctct gggtcagatg
     1681 aaagaaatgg ctgcgaaata cggctacgac atctctggtc cggctaccaa cgctcaggaa
     1741 gctatccagt ggacttactt cggctacctg gctgctgtta agtctcagaa cggtgctgca
     1801 atgtccttcg gtcgtacctc caccttcctg gatgtgtaca tcgaacgtga cctgaaagct
     1861 ggcaagatca ccgaacaaga agcgcaggaa atggttgacc acctggtcat gaaactgcgt
     1921 atggttcgct tcctgcgtac tccggaatac gatgaactgt tctctggcga cccgatctgg
     1981 gcaaccgaat ctatcggtgg tatgggcctc gacggtcgta ccctggttac caaaaacagc
     2041 ttccgtttcc tgaacaccct gtacaccatg ggtccgtctc cggaaccgaa catgaccatt
     2101 ctgtggtccg aaaaactgcc gctgaacttc aagaaattcg ctgctaaagt gtccatcgac
     2161 acctcttctc tgcagtatga gaacgatgac ctgatgcgtc cggacttcaa caacgatgac
     2221 tacgctatcg cttgctgcgt aagcccgatg atcgttggta aacaaatgca gttcttcggt
     2281 gcgcgtgcaa acctggcgaa aaccatgctg tacgcaatca acggcggcgt tgacgaaaaa
     2341 ctgaaaatgc aggttggtcc gaagtctgag ccgatcaaag gcgatctcct gaactacgat
     2401 gaagtgatgg agcgcatgga tcacttcatg gactggctgg ctaaacagta catcactgca
     2461 ctgaacatca tccactacat gcacgacaag tacagctacg aagcctctct gatggcgctg
     2521 cacgaccgtg acgttatccg caccatggcg tgtggtatcg ctggtctgtc cgttgctgct
     2581 gactccctgt ctgcaatcaa atatgcgaaa gttaaaccga ttcgtgacga agacggtctg
     2641 gctatcgact tcgaaatcga aggcgaatac ccgcagtttg gtaacaacga tccgcgtgta
     2701 gatgacctgg ctgttgacct ggtagaacgt ttcatgaaga aaattcagaa actgcacacc
     2761 taccgtgacg ctatcccgac tcagtctgtt ctgaccatca cttctaacgt tgtgtatggt
     2821 aagaaaactg gtaacacccc agacggtcgt cgtgctggcg cgccgttcgg accgggtgct
     2881 aacccgatgc acggtcgtga ccagaaaggt gcagtagcct ctctgacttc cgttgctaaa
     2941 ctgccgtttg cttacgctaa agatggtatc tcctacacct tctctatcgt tccgaacgca
     3001 ctgggtaaag acgacgaagt tcgtaagacc aacctggctg gtctgatgga tggttacttc
     3061 caccacgaag catccatcga aggtggtcag cacctgaacg ttaacgtgat gaaccgtgaa
     3121 atgctgctcg acgcgatgga aaacccggaa aaatatccgc agctgaccat ccgtgtatct
     3181 ggctacgcag tacgtttcaa ctcgctgact aaagaacagc agcaggacgt tattactcgt
     3241 accttcactc aatctatgta attagatttg actgaaatcg tacagtaaaa agcgtacaat
     3301 aaaggctcca cgaaagtggg gcctttttta gcacgagagc cttttttgtc agctatctat
     3361 actttaaggt gactgccaaa acagactcga cgtagccttc gagctgcgca ccaacacggc
     3421 ctcagatggg ccacatctgg agaaacaccg caatgtcagt tattggtcgc attcactcct
     3481 ttgaatcctg tggaaccgta gacggcccgg gtattcgctt tatcaccttt ttccagggct
     3541 gcctgatgcg ctgcctgtat tgtcataacc gcgacacctg ggacacgcat ggcggtaaag
     3601 aagttaccgt tgaagattta atgaaagagg tggtgaccta tcgccacttt atgaacgctt
     3661 ccggcggcgg cgttaccgca tccggcggtg aagcaatcct gcaagctgag tttgttcgtg
     3721 actggttccg cgcctgcaaa aaagaaggca ttcatacctg tctggacacc aacggttttg
     3781 ttcgtcgtta cgatccggtg attgatgaac tgctggaagt aaccgacctg gtaatgctcg
     3841 atctcaaaca gatgaacgac gagatccacc aaaatctggt tggagtttca aaccaccgca
     3901 cgctggagtt cgccaaatat ctggcgaaca aaaatgtgaa ggtgtggatc cgctacgttg
     3961 ttgtgccagg ctggtctgac gatgacgatt cagcgcatcg cctcggtgaa tttacccgtg
     4021 atatgggcaa cgttgagaaa atcgagcttc tcccctacca cgaactgggc aaacacaaat
     4081 gggtggcgat gggtgaagaa tacaaacttg atggtgttaa accaccgaag aaagaaacca
     4141 tggaacgcgt gaaaggcatt cttgagcagt acggtcataa ggtaatgttc taatttcccc
     4201 gtaaagcggc aacttttttg agttgccgct tttttattcc gcgctgcata aataattcag
     4261 agctctattt ctaacgccct tacagcacag cttccaaatg tattacccaa accgccagta
     4321 tatgtcctgc ggcttgcgcg catttctcga ttatgtaaaa acgggtcaga tctgccagag
     4381 tcagcgtcac cgaccacaat aattatttgc tattcgcttt acgcgccagc agcgggaata
     4441 tcagccctaa tcccaccagc acaaacggcg ttgcaacatt cagcgccaac tggaatgtcc
     4501 actctgcggt gaaggcttcc attttcggga aaataccagt caggcaggca aaagcggtaa
     4561 aggcaaaaca ccaaattccg acggtcattg ccagcggctt attacgaata aagacatatt
     4621 ccggcttata tttctgcgcc aggcgaacaa ctgcaataaa tgcaacaaat acccataaat
     4681 aacgcagcgg catcactacc gaattaaggt tcaacagcca tttatagaga ttgttcatat
     4741 caccaatgcc gagagtcggc agcattatca ggatcgccac cagcaccagg gtcagaaaat
     4801 agccattaac gggcgtacca gaagcgttgg tacgacataa actggctgga atatatttgc
     4861 tgtcagcatc gcctaataac actttaagcg gcgcatcaat cgagaatacc agcgcagcta
     4921 cttgtcccag ggtattcgca atggcataaa tcaccattaa agtattaccc atgttgtaat
     4981 actcgcccag cttctgaaag gcgtaatact gaccgttagt cattaagtca tccgggatat
     5041 tacgcgagtc aaacatcatc cccatcgcca gtgagcccag aatggcacaa accgcaacca
     5101 tcaccgcaag gcataacatc ccttttggaa attcttttcc tgggttgcgc gtttgattaa
     5161 cgtaaggaga aatcttctct gctccgccaa ccgcgaaaac cagcattgaa atagtggtaa
     5221 tgtaggtaaa gtcgatatga ggaatgaacg tttcccaggt aatgtttgtg gtcgcaatat
     5281 gcacttcagt aatcgcaggc gcggttaccg ccatcgccac atacaggagc gacataacaa
     5341 acatcgcaat ccctgccaca gaaccgacga ttttcagcga tttcataccg cgtgaagcaa
     5401 cccacataaa gaagataaac agcaccagcg ttaacccctg taacgctacg actgaatatt
     5461 ctttgattaa cgaaccgtcg cctttcatcg cccaaccgag cgcaatcagt attgcctggg
     5521 gtttttgtgc cagataggga atatgcacta cccagtaggt ccacgcggcg aggtaagcca
     5581 gtccgggtcc catcgtgtgt ttaatccagg tactgacccc gcccttccca tctttgaagg
     5641 tcgagcctaa ctgaccaaca attagcgcat aaggtgtgaa atagagcgca aagataaaca
     5701 cccatgaaaa aacaaccacc agcccctggt tggcgtagtt attaacaacg ttgccaaaac
     5761 cccagacagt gataaaagac atcagcgcaa tgttgtacca tcgcagctgt ttttcctgaa
     5821 cattcccagc catagacaaa tccttcgcca aaattaaagg ggcatctgta aaacgaggga
     5881 ttatgcgcgg caaataagaa gtgcggaaat agcgaaatgc aaaattataa cctgctgcac
     5941 attttatttt taaacgaata aaatttggaa taaagcaagt cagatagaag aaacaaaaaa
     6001 gcagagtgtg aaacagtaag ttcacactct gcttgaattc atttacacgt gagcaacggg
     6061 tttcggcgta tgaccggcgt tgcgaagcag catcagcaaa tagataaacg atacgctggc
     6121 gatcatgata aacaataaat tatcggagaa attctgcatt agcatagcgg taaatgacgg
     6181 gccaagcaga cttcccacgg tatagctcaa cagtaaggcc tggttcatcg ccaccagttg
     6241 atgatgttca actttctcgc aagcccaggc catcgccacc ggatatagcg taaagccagc
     6301 ggcaccgagg atgaataacg ctggggccat cgccgcctgg ctaagcatcg cgatactgcc
     6361 gagaatgacg acaaagacct gaacacgcaa caccagcagt cgaccaaact tatccgccag
     6421 acgtccaatc ggccattgtc caaggatacc cgcactgacc agtaccgcca tccagaaacc
     6481 aatgctggca ttgctcaccc ctttgtgatt gaggtacagc ggcatcaggc catatagaga
     6541 tcccagaacg attcctgaga taatgcagcc attcacgcca agccgcgcct gacggagttt
     6601 tagcattgac gtaattgacg tcgaatcatg gttttcagcc tgctgattca gcacacgcgt
     6661 aaacaacagc ggtaagatcc ctgccaacgt caaacctgta acccacggca atacggacat
     6721 cagctcggtt gaaactttgc tgaccagtaa ctggcctaaa aacgttccca cgtaataaac
     6781 catcatatac gcagcaagca aacgcccacg gttacgtgac gtcccactgc acatcagcgc
     6841 gctctcaaca accacccaaa tcatggcaca gccgacgccg gcgacaaaac gccaagccaa
     6901 ccagctccag aatccaatca tcaggccaag gccggcacag ccagcggcaa aaatgaagga
     6961 ggccagataa tagctgcggt taaagccaat gcgcttaatg acataccctg tcagcaatgt
     7021 accgaccagg ttgccggtaa aataggatga gctgacaacg cctacctgcc atgtggacat
     7081 gtgttcctgg gcgagccaaa gcggcacgag tgtatttaac actgcgatcg ccagagtcaa
     7141 caaaagcagg ccagacagca aaagcatgac aggccgggta tacgtggaca tgaattaaaa
     7201 ccgtgaggaa gttcaaattt catgcgcatc atgccaccgc caaaaacatt gtcaatgggt
     7261 gaaaatacgc gcatatcgca gcggaggtat tatcgattta tattactcat tcataacgct
     7321 aatgctggag agacgattaa tctcatctga ttgtttttat tgatctaaca caaatttcat
     7381 acgcttcgtt cagtcgaaaa atttcatggt cacagcaaac tcatatttcc cgtctatgct
     7441 ttcagagcaa tgcatcgcgt ctgaccgatt tcagacgtac cactgactct cgaaaggaga
     7501 agaggatgac caaaccgtat gttcgtcttg ataaaaatga tgctgccgtt ttgcttgttg
     7561 atcaccaggc tggtttactt tcccttgtac gggatatcga acccgataag tttaaaaata
     7621 acgtgctggc gctgggtgac ctggctaagt atttcaactt accaactatt cttaccacca
     7681 gttttgaaac cggtcctaac ggcccattag ttccagaact gaaagcacaa ttccccgatg
     7741 caccttacat tgctcgcccg ggcaatatta acgcctggga taacgaagat tttgtaaaag
     7801 cagtcaaagc gacaggtaaa aaacagttaa ttattgccgg tgtggtaacc gaagtttgcg
     7861 tggcattccc ggcgctttca gccattgaag aagggtttga cgtctttgtc gtgactgacg
     7921 cgtcagggac ctttaatgaa attacccggc attcggcgtg ggatcgcatg tcgcaagctg
     7981 gtgcgcaatt gatgacatgg ttcggcgtgg cttgcgagct gcatcgcgac tggcgtaatg
     8041 atattgaagg actggcgacg ttgttctcta accatattcc tgattatcgt aatttgatga
     8101 ccagttatga cacgttaacg aagcagaaat aaataaatat cctcc
//
LOCUS       DAOTRK010000184         8136 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.185, whole genome shotgun sequence.
ACCESSION   DAOTRK010000184 DAOTRK010000000
VERSION     DAOTRK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.185"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            191..709
                     /locus_tag="QIE41_004210"
     CDS             191..709
                     /locus_tag="QIE41_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDS3533014.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            919..3060
                     /locus_tag="QIE41_004211"
     CDS             919..3060
                     /locus_tag="QIE41_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDS3533015.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFLYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFACWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIVKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3136..7636
                     /locus_tag="QIE41_004212"
                     /pseudo
     CDS             3136..7636
                     /locus_tag="QIE41_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            7636..8013
                     /locus_tag="QIE41_004213"
     CDS             7636..8013
                     /locus_tag="QIE41_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533016.1"
                     /translation="MNQYIANYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQI
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSIDDELATVVATGLIEAIVTATDSNSLLWK
                     DVSKFLGDKSKSHALVWKDFGQE"
BASE COUNT         1985 a         2011 c         2578 g         1562 t
ORIGIN      
        1 cttattgctg aataagagat ttaatgattt taaaaatagt ttttacgaca accgtaaatt
       61 ctagctcggt ttgtatgtaa aaaatactga aaaatgatgc ggttccgtga agtgagttgc
      121 atctgcctgt ctgggattaa cggcaggtca ttgtaagtag taattaacca cgttaacaag
      181 gagtttacaa atgccaacac cgtgttatat ctccatcact ggtcagaccc agggaaatat
      241 tactgcaggt gcatttaccg ctgattctgt cggcaacatc tatgtgcagg ggcacgaaga
      301 cgagatgctg gtgcaggaat tcctgcataa cgttactgtc ccgaccgatc ctcagtccgg
      361 ccagcctgcg ggtcagcgtg ctcacaagcc gttcattttt accgtggcgc tgaacaaagc
      421 tgttccgctg atgtacaacg cactggcctc cggcgaaatg ctgccaacca ctgaacttca
      481 ctggtggcgt acctctgtgg aaggcaaaca ggagcattat ttcaccaccc gcctgaccga
      541 ttcgacgatt gtcgatatga aactgcatat gccgcattgc caggatccgg cgaaacgcga
      601 gttcacccag ttgctcgaag tttcactggc ctaccgcaaa atagagtggg aacacgtcaa
      661 gtctggcacc tccggtgccg acgactggcg cgcaccgctg gaagcataag ttaagccaac
      721 agcatccggc tattaggccg gatgctgttt ttgtattacg ccgtaaaaag aaaggttgtc
      781 agtaaggggt tacgttcacg ggagatggcg gatgccggaa acttcggcgg tagcgtgcct
      841 gaacgcaagc gtaacactgt actgttacag ccgctttaac agcgaactgt ctgtttttcc
      901 ttaaacccgg aggttgctat gtcaaccgga ttacgtttca cgctggaagt ggacggcctg
      961 ccaccggacg cgtttgcggt ggtctccttt catctgaacc agtcactctc ttcgctcttt
     1021 tccctcgatc tctctctggt cagccagcag tttctctccc ttgaatttgc gcaggtgctg
     1081 gacaaaatgg cctacctgac ggtatggcag ggcgatgacg tacagcgccg ggtgaaaggt
     1141 gtggtgacct ggtttgaact gggggagaac gacaaaaacc agatgctgta cagcatgaag
     1201 gtgtgcccac cgctgtggcg cacagggctg cgccagaact tccgtatctt ccagaatgag
     1261 gacatcgaaa gcatactcgc gacgatcctg aaagaaaacg gggtgaccga gtggagtccg
     1321 ctgttcagcg agccgcatcc ttcccgtgag ttttgtgtcc agtacggcga aaccgattac
     1381 gatttcctgt gccggatggc ggcggaggaa ggcatcttct tttatgagga gcacgcgcaa
     1441 aaaagtaccg accagagcct ggtcctgtgc gacaccgtgc gttatctgcc ggagtccttt
     1501 gagatcccct ggaatccgaa cacccgtacc gaggtgagca ccctatgcat cagccagttc
     1561 ctctacagcg cacaaatccg cccatcttcc gtggtgacca aagactacac ctttaaacga
     1621 cccggctggg cagggcgttt tgatcaggaa ggccagcacc aggattacca gcgcacacag
     1681 tatgaagtgt atgactaccc cggacgtttc aagggtgccc acgggcagaa ctttgcctgc
     1741 tggcagatgg atggctggcg aaacaacgca gaagtggcgc gcggaacaag ccgttcgccg
     1801 gagatatggc cgggacggcg aattgcgctg acggggcatc cgcaggcgaa cctgaaccgg
     1861 gaatggcagg tggtggcaag tgaactgcac ggcgagcagc cgcaggcggt gccgggacgc
     1921 aggggttcag gtaccacgct gaataaccac tttgcggtaa taccggcaga cagaacatgg
     1981 cggccacagc cgttgctgaa accgctggtg gacggcccgc agagcgccgt cgtgacggga
     2041 ccggcaggag aagaaatctt ctgtgatgaa catggccgcg tgcgggtgaa atttaactgg
     2101 gaccgctata acccgtcaaa ccaggacagt tcatgctgga tccgtgtggc acaggcgtgg
     2161 gcaggcaccg gctttggcaa cctggcgata ccgcgtgtgg gtcaggaagt cattgtggac
     2221 ttcctcaacg gcgatccgga ccagccgatt attatggggc gcacctacca ccaggaaaac
     2281 cgcacccccg gcagcctgcc gggaacaaag acgcagatga ccattcgttc aaaaacctat
     2341 aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga acaggtctac
     2401 atccacgcgc agaagaacat gaacaccgag gtgctgaata accgcaccac tgatgtgata
     2461 aacaaccatg ccgaaaaaat aggtaacaac caggcgatca ccgttaccaa taaccagatc
     2521 cagaacattg gcgttaatca gatacagacg gttggtgtca accaggtgga aacggtaggc
     2581 agcaaccaga ttgtcaaagt gggttcaaac caggttgaaa aggtggggat cattcgtgcg
     2641 ctgacggtgg gcgtggctta ccagacgacg gtaggcggca tcatgaatac ctcggtggcg
     2701 ttgttgcagt cctcacaggt agggctgcat aaatcactga tggtgggaat gggctacagc
     2761 gtcaatgtag ggaataacgt caccttctcg gtgggcaaga cgatgaagga aaacaccgga
     2821 caaacagcag tttattctgc cggtgagcat ctggaactct gctgcggtaa ggcaaggttg
     2881 gtgctgacga aggacggaag catatttctc aatggtacgc acattcatct ggaaggagag
     2941 tcggatgtga acggtgatgc gccggtgatt aactggaact gtggtgccac acaacctgta
     3001 ccggatgcgc ctgtgccgaa agatttaccc cccggaatgc cggatatgcg gcaattttga
     3061 gtcaggtgca aaaaattaaa acggaactgg tgcagtgacc agataaacca tattaaaagc
     3121 agaaggaatt gattcatggg tggaaaaccg gcggcgcggc agggtgacat gacccgcaag
     3181 ggactggata ttgtgcaggg ttcagcaggg gtgctgatag gtgcgccaac cggtgtggcc
     3241 tgctcggtgt gtcccacagg ggaagccacg ccgaaatatg gcaatccggt aaacccgctg
     3301 ctgggagcga aggtgctgcc tgctgaaacc gacctggcac tgtccggccc gctgccgttt
     3361 gttcttttcc gcgcctacag cagctaccgg accaggacgc ccgcgccggt ggggggggtt
     3421 ggtcccggct ggaaagcccc cttcgatatc cgcttacaga tacgcgatga aggcctgata
     3481 ctcaacgaca gcggcggcag gagcattcac tttgaacccc tgtttcccgg cgagataagc
     3541 tacagccgca gcgagtcgtt ctggctggcg cggtgcgggg tgctgaaaca gcacaaaggc
     3601 cacccgcttg cgcggctctg gcgggcgttg ccggaagcgg tccgtctgag tccacacatc
     3661 tatatgatgg ctgccagcac aaccgggcag tggctggtgc tcggctggcc ggagcgggtg
     3721 ccgggggcgg acgaggtgct gccgccggag ccgcccgcat accgggtgct gacgggcgtg
     3781 gtggacggct tcgggcggac gctggccttc caccgggcgg cagagggtga cgtggcgggt
     3841 gcggtgacgg gggtgacgga cggcgcgggc cgctgttttc acctggcgct gagcacgcag
     3901 gcgcagcggg cggaagcctt ccgtaaacag cgcgcctctt ctttatcttc tccagccggt
     3961 ccccgttctg tttcctcttc tcaggttttc cccgatacgc tgcccgccgg tacagaatac
     4021 ggtgccgata acggtatccg gctggaggcg gtgtggctga cacacgaccc ggcatacccg
     4081 gatgaacagc cgactgcgcc gctggcgcgc tatacgtaca cggccagcgg agaactgcgg
     4141 gcggtgtatg accgcagcgg gacgcaggtg cgcgggttta cttatgatgc ggagcacgcc
     4201 gggcggatgg tggcgcacca ttatgcaggg aggccggaga gccgctaccg gtatgatgat
     4261 accgggcggg tgacggagca ggtcaacccg gaggggctgg actaccgctt tgagtacggg
     4321 caggaccgtg tgaccatcac ggacagcctg aaccggcggg aggtgctgta cacggaaggc
     4381 gggggtggcc tgaagcgtgt ggtgaagaag gagcatgccg acgggagcat cacccgcagc
     4441 gagtatgatg aggcggggga ggctgaaggc gcagacggat gcggcaggac ggcggacgga
     4501 gtaccggctg catatggcgt ctggaaagct gacttcagtg atactgccgg acggcaggac
     4561 ggtacggtat ggttacaaca gccagttgca actgacttca gtgacatatc cggatggttt
     4621 acgcagcagc cggaaatatg accgtcaggg ccgactggca gaagagatct cgcgcaacgg
     4681 taacattacg cgctggtttt atgactcccc ccgcagtagc ctgcccggcg cggtggaaga
     4741 cggaatcggg gtgacgcgga gaataacgcg aaaccggtac ggtcagcttc gggccttcac
     4801 ggactgctcg gggtacatga cgcggtatga gtatgaccgg tacggtcaac aaatcgccgt
     4861 tcaccgggag gaaggcatca gcacttacag cagttataac ccgcgaggcc aactggtcag
     4921 tcagaaggat gcgcaggggc gtgaaacccg ttatgagtac agcgccgcag gcgacctgac
     4981 agcaacgata tcaccggacg gtaaacgcag caccatcgaa tatgataaac gcgggcgtcc
     5041 ggtgagtgtg acggaaggcg gtctgacgcg cagcatgggg tatgacgctg ccgggcgcat
     5101 caccgtgctg accaacgaga acggcagcca gtccacgttc cggtatgacc cggtggacag
     5161 gctgacggag cagcgcggct ttgacggcag gacgcagcgt taccggtaca gcgccacagg
     5221 gcagcttgtc cacagtgaag acgagggact tatcaccctc tggcattacg atgcgtcgga
     5281 ccgcatcacg caccggacgg tgaacggcga cccggcggag cagtggcagt atgatgagca
     5341 cggctggctg acagaaatca gccatatcag cgaaggccac cgtgtggcgg tccactacgg
     5401 ccacgacgat aaaggccgcc tgacgggcga acggcagacg gtggagaacc cggagacggg
     5461 ggagatgctg tgggagcatg agacgaagca cgcgtacagc gaacaggggc tggcgacccg
     5521 tcaggagccg gacggtctgc cgccggtgga gtggctgacg tatggcagcg gttatcttgc
     5581 gggaatgaag ctgggtggta cgccactggt cgagtacacg cgggaccggc tgcaccgtga
     5641 gacggcccgc agcttcggcg gggaggcata tgagcttgcc accgcctgga ataccagcgg
     5701 ccagctccgg agcaggcacc tgaaccttcc gcagcttgac cgtgactacg actggaacga
     5761 caacggacag ctaattcgca tcagcggccc gcaggagagc cgggagtacc gttacagcga
     5821 cacgggaagg ctgacgggcg tccacaccac ggcagcgaac ctggacattg atatcccgta
     5881 tgcgacggac ccggcaggaa accggctgcc ggacccggaa ctgcacccgg acagcaccct
     5941 cacggcatgg ccggataacc gcatcacgga agatgcgcac tatgtctacc gctacgatga
     6001 atacggcagg ctggcggaga agacggaccg tatcccggaa ggggtgatac ggatgcacga
     6061 cgagcggacg caccactacc attacgacag ccagcaccgc ctggtgttct acacgcggat
     6121 acagcacggc gagccacagg tggagagccg ctacctctat gacccgctgg gccgccggac
     6181 gggaaaacgg gtgtggcggc gggagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa
     6241 accggaggtg acctggtacg ggtgggacgg cgacaggctg acgacggtac agaccgacac
     6301 cacgcgtatc cagacggtat accagccggg gagcttcacg ccgctcctga gaatcgaaac
     6361 agagaatggc gaacaggcga aggcgcggca ccgtagcctg gcggaggtgt tgcaggagga
     6421 cacgggtgtg acgctaccgg cggagctggc ggtgatgctg gggaggctgg agcgggaact
     6481 gcggcagggc agcgtgagtg aagaaagcca gcagtggctt gcacagtgtg ggttgacggc
     6541 ggaacagatg gcggcgcaac tggaggcgga atacatcccg gagaggaaac ttcatcttta
     6601 ccactgtgac caccgtgggc tgccgcaggc gcttatcagc ccggaagggg aaacggcgtg
     6661 gcgcggggag tatgacgagt ggggaaacct gctgggagag gaaagcacgc agcaccttca
     6721 acagtcactc cgtctgccgg ggcagcagta tgatgaggag tcggggctgt actacaaccg
     6781 tcaccggtac tatgacccgc tacaggggag gtatatcacc caggatccga ttgggctgaa
     6841 ggggggatgg aacctgtatg cgtatccact gaacccggtg caacggctgg atccgctggg
     6901 attaagtgcg gcgttgggcg caggagcatt aactgccatg ggaactggtt ctgaagtagg
     6961 tacagagatc ggttcggtag gaggccctcc ggggatgtta gtcggtgcgg cagtcggtac
     7021 ggtggttatt ggtggtggta taataatata caacaatcgc gaggctgttg gggacgcgat
     7081 tgagaaaagt atggagatgc agggtgaagc cgaattatta aatgcacaaa gtcaaatgga
     7141 attctggcga gatccctatg gtgctttttc cagcgtgttt ggaaatgatg attcatcaaa
     7201 agagaatcca aatgtcggga aggatttgac ggaggaagag aagaactgcc tgggtggtgg
     7261 cagtacggga agcccgggag gatggggacc agaagatgag aatagcgcaa gagatcgtat
     7321 gcagcatgaa cggtataaag atgaattgcg acagaagatg ggaaaacctg acgttacaga
     7381 ttcaaatttg aaaaatatta tagacgatct ttatcggcca aatgcaaaag taggaagtgg
     7441 aagcacagca gatgctgttc gttatgaatt ggccacaggt gaaaaagttg gtggtagagg
     7501 acatgtattg aaagcacagg attatacaag ggctttacag gattggttgg ataagaatcc
     7561 aacagcatct ccaggggatc gagcagcagc agaaaacgtt cttcaggatt tacagaatgc
     7621 tttgggaggc aagtaatgaa ccaatatatt gctaattata tagaaaaaat ggcatcgttt
     7681 tccactgttt tgtctgagac agtctcatta gctaatgaat actggcatcc agaaactcca
     7741 ccggtggtta tgttgttctc tcagattgga aaaacgattg taaaaaattt ttatgaaatg
     7801 ggtgatattg aaagagaaaa aatatttaaa catgtcgaag atggtatggt ctcgattgat
     7861 gatgaattgg ccacagttgt agctacaggg cttatagagg caatagtcac tgcgaccgat
     7921 agcaattctt tactttggaa agatgtaagt aaatttttgg gtgataaatc aaagtctcat
     7981 gcattagtgt ggaaagattt tggtcaggag tgatattctt taagaagtat taagttcgct
     8041 attgaggcct taatacttct tgaagttaaa ttaaagttag ggtgaagtgc agggaaagat
     8101 cacgtaacgc tgcttttttg tactaaataa ttcgca
//
LOCUS       DAOTRK010000185         8022 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.186, whole genome shotgun sequence.
ACCESSION   DAOTRK010000185 DAOTRK010000000
VERSION     DAOTRK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.186"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(134..286)
                     /locus_tag="QIE41_004214"
     CDS             complement(134..286)
                     /locus_tag="QIE41_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDS3533017.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(551..1285)
                     /gene="cysH"
                     /locus_tag="QIE41_004215"
     CDS             complement(551..1285)
                     /gene="cysH"
                     /locus_tag="QIE41_004215"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDS3533018.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRTNLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1359..3071)
                     /gene="cysI"
                     /locus_tag="QIE41_004216"
     CDS             complement(1359..3071)
                     /gene="cysI"
                     /locus_tag="QIE41_004216"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDS3533019.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3071..4870)
                     /gene="cysJ"
                     /locus_tag="QIE41_004217"
     CDS             complement(3071..4870)
                     /gene="cysJ"
                     /locus_tag="QIE41_004217"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDS3533020.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVNVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5189..5551
                     /gene="queD"
                     /locus_tag="QIE41_004218"
     CDS             5189..5551
                     /gene="queD"
                     /locus_tag="QIE41_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDS3533021.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5629..6900
                     /locus_tag="QIE41_004219"
     CDS             5629..6900
                     /locus_tag="QIE41_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS3533022.1"
                     /translation="MEDNCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6891..7151
                     /locus_tag="QIE41_004220"
     CDS             6891..7151
                     /locus_tag="QIE41_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDS3533023.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7168..7743
                     /locus_tag="QIE41_004221"
     CDS             7168..7743
                     /locus_tag="QIE41_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDS3533024.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            7878..>8022
                     /locus_tag="QIE41_004222"
     CDS             7878..>8022
                     /locus_tag="QIE41_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3533025.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGP"
BASE COUNT         1787 a         2144 c         2082 g         2009 t
ORIGIN      
        1 ataattttca tccgtaatat atatgaataa taaacatagg cataatattc acataaacaa
       61 tataaattaa aaaagggtgc aggagaccaa accatcacaa ttcaatacgc ggggttttac
      121 ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac tgccttaaac tccataccac
      181 gttctctgat actcaactca cacaacgagt cgcctaccat cagcgtgaat cccagtactg
      241 tacaacacag tacgatgatt gccacaaggg catattttgt cagcatgtct tgcctccctg
      301 caaagaagag actaacattc gaattgctta ggttcgaacg ctggcctcag gttgatagaa
      361 atatcgcctg gggcttttgt ccatctggaa cctcgcgaat gcttaacgcc agacagcctc
      421 aagcacccaa cgccattcta tacctgataa ttcttccagc ggcgttcacg tttaaaatca
      481 ggaatgctgc tcgcaaaata aaaacgcccc catcatttct gacagaggcg tttaatttat
      541 ccggcaatat ttacccttcg tgtaacccac attctctttt taagccaaag aaacgcgttt
      601 cttcttccgc catgccgggt tcccatttac gggttgtatg ggtatcaccg accgataaat
      661 atccttcatc ccataatggg tgatatttca ggccatgttt ttgcaggtac tgataaatag
      721 ttcggttatc ccagtcgata atcggcagca ctttaaatac gccacgctga attgccagca
      781 ccggtaaatt ggtgcggctg ccggattgtt cacggcgcag gccagcaaac caggtttgcg
      841 cattcagttc tttcagcgcc cggttcatcg gttcgacttt gttgatgtca ttgtactttt
      901 caatgccttc aacaccctgc tcccacagtt ttccgtaacg tgcttcctgc caggcggcgc
      961 tttcggtagc gcggtacact ttcaggttga gcttgagttt gtccgttaac tcgtcaataa
     1021 agcggtaggt ttccgggaac aagtaacccg tatcggtgag gatcactgga atatccgggc
     1081 gaatctgatt caccagatgc aggctcaccg ccgcctgaat gccgaaactg gaagaaagta
     1141 catattcacc gggcagatta tccagcgccc aggcaacgcg gccttcagcg tccagttttt
     1201 ccagttcggc gttagtttct gccagcgcca gaatgcgatc taccttcggc agttcgttca
     1261 gggcgtttag atcgagtttg gacatagatt cctcactgtt tgccttgcct gatgcgacgc
     1321 tcacgcgtct tatcaggcct acaagaccgg gctgatggtt aatcccacaa atcgcgcgcc
     1381 ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa agtcgccgaa gccttcaccc
     1441 gcttcgcgct ctttcgccca gcgccctatc agttcatcaa gcgacgccag gatttccggt
     1501 tcggtgatgt tttctttata catccgtggg atacgtgtcc caatgcggtt gccgccaaga
     1561 tgcaggttgt agcgacccgg cgctttaccc accaggccca cttccgccag catcgcgcga
     1621 ccacaaccgt tcgggcagcc tgttacacgc atcacgatat gctcatcgct gacaccatgt
     1681 ttcgccatta aattatcgat gttgtcgata aaagacggca ggaaacgctc ggcttcggcc
     1741 atcgccagcg ggcaagtcgg gaatgacacg caggccattg agttttcacg ctgcggcgtg
     1801 acggcattca ttaacccgct ctctttggca atcttctcga tcttcgcttt ctcgctttcc
     1861 ggcacaccgg cgatgatcag attctggttc gccgtaatgc ggaaatcgcc tttgtggatc
     1921 ttcgcgatct ccagcaggcc ggttttcagc ggacgcccag gataatcaag gatgcgacca
     1981 ttttcgataa acagcgtcag gtgccagtta tcatcaatgc ctttaaccca gccaatacga
     2041 tcgccgcgtc cggtgaactc atatggacgg atcggttcaa atttgatccc cgcgcgacgc
     2101 tccacttccg ctttaaacgt ctcaaccccc acgcgctcca gcgtgtattt ggttttggca
     2161 tttttacgat cggtacggtt accccagtcg cgctgagtcg tcacgacggc ttctgccacc
     2221 gccagcgtat gctccagcgg cagatagcca aactcactcg ccgtgcgggc gtaggttttc
     2281 ttgttgccgt gttcgataga aagcccaccg cccaccaaca ggttaaagcc caccagcttg
     2341 ccgttttcgg cgattgccac gaagttcatg tcgttggcat gcagatcgat atcgttctgc
     2401 ggcgggatca ctaccgtagt tttgaattta cgcggcaaat aagtctgacc gaggatcggt
     2461 tcttcatcgg tggtggcgac tttttcctga tcgagccaga tctccgcata cgcgcgggta
     2521 cgcggcagca gatgttcaga gattttcttc gcccattcat acgcttccgc gtgtagctgc
     2581 gactcgtaag ggttcgaggt gcagagtacg ttacggttca tgtcgttagc agtcgccagc
     2641 gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt tgacgttctt tttcagaatg
     2701 ccgtggaact gaaacgtctg gcggttggtc aggcgaatgc tgccatagat ggtgttttca
     2761 ccggcaaatt tatcgatcgc ctgccactgt ttggtggtaa tcacgccacc cggcagacgg
     2821 cagcgcaaca gcattgcatg acgtggctcc agcttctgtt cagcacgttc ggcgcggatg
     2881 tcgcggtcat cctgctgata catgccgtgg aagcggatca gcaggaagtt atcgccctta
     2941 aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg tgccgcgcag gtagttgctt
     3001 tcaagcttca tgcgctcggc gtctgtcagt tttccttcga ccactaaagg ccctggatgt
     3061 ttttcgctca ttagtagaca tctcgctgat aacggcgctc tacgcgcagc tcacttaaaa
     3121 attcatccgc cgcttcggtg tccatgccac caaattcggc aatcacttcc agaagtgcct
     3181 gctcaacgtc tttcgccatg cgattagcgt cgccgcagac ataaatgtgg gcaccgtcat
     3241 tgatccagcg ccacagctct gcgccctgtt cgcgcagttt gtcttgtacg taaatttttt
     3301 ctttttgatc gcgcgaccag gcaagatcga tacgcgtcag cacgccctct ttgacgtagc
     3361 gctgccattc cacctggtag aggaaatctt ccgtaaagtg cgggttgcca aagaacagcc
     3421 agtttttccc cggcgcttcg tcggcggcgc gttgctgcat aaaggcgcgg aacggcgcga
     3481 tgccggtgcc tgggccaatc ataatcaccg gcgtttgcgg gttagcgggc aggcggaagt
     3541 tatcgttatg ttcgataaag acgcggactt caccctcttc ttccacgcga tccgcgagga
     3601 agctggaggc accaccggca cgggcgcggc cttccacgtc gtaacgcacc acgccaacgg
     3661 tgacgtgtac ttcgttctcg acttccgcct gcgaggaggc gatggaatac aggcgcgggg
     3721 tcagcgggcg cagcaggtta atcagcgctt cggcatccag ttgcgccgga gagaaacgca
     3781 ccatgtcgac aatcggcgtc gtcgcggcgt aatgctgtaa cttcgcttta tcgcccacca
     3841 gcggcagcag cgtttcgctg cgggtaagcg tggcatagtt ctcaacaatg ttggcggtgt
     3901 tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag cggcagggtt ttgccctcaa
     3961 cgttgacagg ttcatcacct ttcagccaca gcagttcgac aagttctttc accagtgccg
     4021 gatcgttctg ataccagacg cccagcgcgt cacccggctg gtaacgcagg cccgagtcac
     4081 ctaagtcaat ttcgatatgg cgaacgtctt tttcagagtt acgcccggta attttctggt
     4141 taacagagag gctcgccacc agcggcgcgt ctttgctgta cgggctggtg tggatttcat
     4201 ttaccgcgcc agtagcgacg gattgcgaag gtgccgcgac aggcgcacgc gatttaagcg
     4261 catcaaccac gcgggcgcgc cactcgatgg cagcagcctg gtattcaacg tcggcatcga
     4321 cacggtcgag caggcgttca ccgcccagtt ccgccagctt gctgtcaaaa tctttcccgg
     4381 actggcagaa aaattcataa gagctatcgc cgaggctaaa cacggcaaat gcggtgtttt
     4441 ccagcttcgg cgcttttttg gagaacagga acttatgcag cgcgacggct tcttccggcg
     4501 gttccccttc tccttgcgtt gacgtcacca cgataagcag tttttcgctg gcgatttgtt
     4561 tgaatttata gtcgcccgcg ttcaccagct taacgttcag ttttgccgcc aataaatcat
     4621 cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg cgaagcggag ataatagtta
     4681 tacccggcat ttctgcggcg ggcgctggcg tcgctgcaag cgcagctggc tgctggttga
     4741 gcacgcccca gaaatagcca gaaacccagg caagctgggt gggagttaaa tcggtcgtgg
     4801 ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag caacgcggaa ggtgggacct
     4861 gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt ttctgcgccc tgtcagcgcc
     4921 cataaaacag aagagaagat aaggttaacg gggtgaacgg tgtggattaa agacgggata
     4981 gcgataacta ataaccaaat cgactaacct gttttagcaa tagtctttaa caacaaaata
     5041 gattaaccaa cccaatgaaa aacaaatgaa tattgcccat caataagata aatcagcgat
     5101 tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag cctttccggt actctacggc
     5161 ggttttatcg tcctgtagag aaattatgat gtccaccacg ttatttaaag atttcacctt
     5221 cgaagccgct caccgcttac cacacgtccc ggaagggcat aaatgtggtc gcctgcacgg
     5281 gcattccttt atggtgcgac tggaaattac cggggaagtc gatccgcata cgggctggat
     5341 tatcgatttc gctgaactga aagcggcgtt taagccaacc tacgagcgcc tcgatcacca
     5401 ttatctcaat gatattccag gtctggaaaa cccaaccagc gaagttttag caaaatggat
     5461 ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg gtgatggtaa aagaaacctg
     5521 tactgcaggc tgtatctatc gcggcgaatg ataagagtgt gtcggcggtc aatttccctt
     5581 aagtaacgcc atgttagggt gttgtgttct ggatatctgg ggcatgacat ggaagacaac
     5641 tgcgacatta ttattattgg tgccggtata gcaggtaccg cttgtgcttt acgctgcgcg
     5701 cgggcaggtt tgtccgttct gttactggaa cgcgctgaaa tccccggcag caaaaatctt
     5761 tccggcgggc gtttatacac ccatgcgctc gcggaactcc tcccgcaatt tcatctgacc
     5821 gcgcctcttg aacgacgcat cactcacgaa agcctttctc tgctaacgcc ggatggcgca
     5881 acgacgtttt ccagcttaca gcccggcggt gaatcctgga gtgtattacg tgcacggttc
     5941 gatccgtggc tggttgccga agccgaaaaa gaaggtgtcg aatgcatccc cggtgcgacg
     6001 gtggatgcgc tgtatgaaga aaacggcagg gtgtgtggtg tcatttgtgg tgacgatatt
     6061 ctccgcgccc gttatgtggt gctggcagaa ggtgccaaca gcgtcctggc tgaacgtcac
     6121 gggttagtga ctcgtcctgc tggcgaagcg atggcgttgg ggatcaaaga agtgctgtcg
     6181 ctggaaacat ccgctattga agaacgtttt catctggaga ataacgaagg cgcagcgttg
     6241 ctgttcagcg gcgggatctg tgatgactta cccggcggcg catttcttta tactaatcaa
     6301 caaacgctct cgttagggat tgtttgcccg ctctcttccc ttacgcaaag tcgtgttccg
     6361 gcaagcgagc tgctggctcg ctttaaaacg catccggcag tgcgcccgct tatcaaaaac
     6421 acggaatcac tggagtatgg tgcgcatctg gtgccagaag gtggcttgca cagtatgccg
     6481 gtgcaatacg ccggtaacgg ctggctgctg ctgggcgatg cgttgcgcag ttgcgtcaat
     6541 accggaattt ccgtgcgcgg catggatatg gcgctaactg gcgcgcaggc ggcggcacaa
     6601 acgctgataa gcgcctgcca gcaccgcgag ccgcaaaatc tgtttccgct ttatcatcac
     6661 aacgtcgagc gcagcctgct atgggatgtt ctacaacgtt atcagcatgt tccggcgctt
     6721 ttgcaacgcc ctggctggta ccgtgcgtgg cctgcgttaa tgcaggatat ttcccgcgat
     6781 ttatgggatc agggtgataa acctgttcca ccgctgcgcc agttattctg gcgtcattta
     6841 cgtcgtcacg gcctgtggca tctggcgggc gatgttatca ggagtctgcg atgtctgtag
     6901 cccgtaatct ctggcgcgtt gccgatgcgc cgcacattgt tccggctgac tccgttgagc
     6961 gccagacggt acaacggttg attaacgctt gtccggctgg tcttttttcg ctcacaccgg
     7021 aaggtgactt acgcgttgat tatcgcggct gcctggagtg tggcacctgc cgtttgctgt
     7081 gcgacgaatc aacactacaa cagtggcgct atccgccttc cggattcggc atcacctacc
     7141 gctttggata aataataagg ataatttatg cccctcttac acctgctccg ccagaatccg
     7201 gtgattgctg ccgttaaaga taatgccagc ctgcaactgg caatcgattc tgaatgccag
     7261 tttatttcag tgttgtacgg caatatctgc accatcagta atatcgttaa aaagattaaa
     7321 aacgccggaa aatatgcctt tattcatgtt gatttgctgg aaggcgcatc gaacaaagaa
     7381 gtggtgattc agtttctgaa actggtgacc gaggcggacg gcattatcag cacgaaagca
     7441 tcgatgctga aagcggcaag agcggaaggt tttttctgta ttcatcgcct gtttattgtt
     7501 gattcgattt cgtttcacaa cattgataag caagttgcgc aatcgaatcc ggattgtatt
     7561 gagatcctgc caggctgtat gcctaaagtg ctgggctggg tgacggagaa aatccgccaa
     7621 ccgctgattg ccggcgggct ggtgtgcgat gaagaagatg cgcgtaatgc gattaacgcg
     7681 ggtgttgtgg cgctttccac cacgaatacc ggggtctgga cgttagcgaa aaaattgctt
     7741 tgacgggata ctcgtaacga gttgaatttg tgtgatttgt aggctgcacg ccacatccga
     7801 cattcagcgc ctgatgcgac gcttgatgaa ctgcacccca aatgttggac ataatctgac
     7861 atctgaaggt gcagtttatg aaacattcat ttgaagtaaa acttgccgct gtaaatcatt
     7921 acctggctgg tcatgcaggc atcatttcta cggcaaaact ctttcaactt tctcacacca
     7981 gcctctcaca ctggattaat ctttttcttc ttcacggtcc tc
//
LOCUS       DAOTRK010000186         8005 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.187, whole genome shotgun sequence.
ACCESSION   DAOTRK010000186 DAOTRK010000000
VERSION     DAOTRK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.187"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            55..1389
                     /gene="adeQ"
                     /locus_tag="QIE41_004223"
                     /gene_synonym="yicO"
     CDS             55..1389
                     /gene="adeQ"
                     /locus_tag="QIE41_004223"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDS3533026.1"
                     /translation="MNNDNTDYLSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGQFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1442..1894
                     /locus_tag="QIE41_004224"
     CDS             1442..1894
                     /locus_tag="QIE41_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDS3533027.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2010..2333)
                     /locus_tag="QIE41_004225"
     CDS             complement(2010..2333)
                     /locus_tag="QIE41_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDS3533028.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2317..2670)
                     /locus_tag="QIE41_004226"
     CDS             complement(2317..2670)
                     /locus_tag="QIE41_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDS3533029.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGINWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2903..4087
                     /gene="nepI"
                     /locus_tag="QIE41_004227"
     CDS             2903..4087
                     /gene="nepI"
                     /locus_tag="QIE41_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDS3533030.1"
                     /translation="MSEFIAENRGADVITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            4288..>8005
                     /gene="air"
                     /locus_tag="QIE41_004228"
     CDS             4288..>8005
                     /gene="air"
                     /locus_tag="QIE41_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HDS3533031.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINPAHSDTAASLILPNVKTIPYTLGALESPPTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDNRTQTNHGIGWRYFTSSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKVVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLAGSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQVKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAETNGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGAIKVTVTL
                     KDSYENLVGGQRDAINLAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SGWANALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSAGIRTDAHGMAEVDLSSTVADASTVEAKINQSSDSKT
                     VNFVADVSTAQVAELVVIKDGSEADGSTANTLRVKVTDAFGNTLAGQTVSVLADNGAT
                     VAPTVITEPDGTVEISVTSQTAGTSVVTASVNNSS"
BASE COUNT         1871 a         1900 c         2149 g         2085 t
ORIGIN      
        1 ttgtcatttg ttttacaaaa gcaagggatt aaatctcctc caatggacaa aaaaatgaat
       61 aatgacaata ccgattactt gagtaatgaa tcagggacgc tttcgcgatt atttaaacta
      121 cctcagcatg ggaccaccgt ccgcacagaa ttgattgcgg ggatgaccac ttttttaacc
      181 atggtgtaca tcgtttttgt gaacccgcaa atcctcggcg cggcacaaat ggacccgaaa
      241 gtggtgtttg ttaccacctg tttgattgcc ggtatcggca gtattgcgat ggggatattt
      301 gctaacttac ccgtggcgct ggctccggca atggggctga acgccttctt tgcgttcgtg
      361 gtcgtggggg cgatgggtat ctcctggcag accgggatgg gcgcgatatt ctggggcgca
      421 attgggctat ttttgctcac gctgtttcgt atccgttact ggatgatctc caacattcca
      481 ttaagtttac gtattggcat caccagcgga atcggattat ttattgcctt aatgggatta
      541 aaaaacactg gcgttattgt cgccaataaa gacaccctgg tgatgattgg cgatttaagt
      601 tctcacggcg tgttgttagg tattttaggg ttttttatta taaccgtgtt gtcatcacgt
      661 cattttcatg ccgcggtgct tgtttctatt gtggtgacgt cttgctgtgg attatttttc
      721 ggtgatgttc attttagcgg tgtctattcc attccgcctg atattagcgg cgtcattggt
      781 gaagtagatt tgagcggcgc gttaacactt gaactcgccg gtatcatttt ctcctttatg
      841 ctgatcaacc tatttgattc atcaggaaca ttaattggtg taactgataa agctggctta
      901 atagatagta acggtcaatt ccccaatatg aataaagcgc tgtatgttga tagcgtcagt
      961 tcggtggcag gcgcatttat tggtacctca tctgttactg cctatattga aagtacttct
     1021 ggtgtggcgg tcggtggtcg cacggggctg acggctgtcg tggtcggcgt tatgttcctg
     1081 ttagttatgt tcttctcacc gctggtggcg atggttcctc cttacgcaac cgccggagcg
     1141 ttaatctttg ttggcgtgct gatgacttcg agcctggcgc gcgttaactg ggatgatttt
     1201 accgaatcgg tgcctgcgtt tattaccacg gtgatgatgc cctttacttt ctcgatcacc
     1261 gaagggattg cacttggctt tatgtcgtac tgcatcatga aagtatgcac cgggcgctgg
     1321 cgcgatctga acctgtgtgt ggtggtggtc gcagctctgt ttgcactgaa gattattttg
     1381 gtggattagt aactggcgag ggtttccaga tatcatgagt tctgattacg caggagaact
     1441 catgatctgg ataatgctcg ccacgctggc ggtagtgttt gtggttggtt ttcgggtgct
     1501 gacatccggg gccagaaaag cgattcgccg tctcagcgat cggctgaata tcgatgtcgt
     1561 acccgtggag tcgatggtcg atcaaatggg aaagtcagcc ggtgacgaat ttttacgtta
     1621 tttgcatcgc ccggatgagt cgcacctgca aaacgccgcg caggtattgc tcatctggca
     1681 aattgtcatt gtcgatggta gcgaacagaa cctgctgcaa tggcatcgga ttttacaaaa
     1741 agcccgcctt gccgcgccga ttaccgatgc tcaggtcagg ctggcgctgg gttttctgcg
     1801 cgaaaccgaa cctgaaatgc aggatattaa tgcttttcag atgcgctata acgcgttctt
     1861 tcagcctgcc gagggcgttc actggctgca ttaaaaccgc ttcgtcttat cagcctacag
     1921 accgcgcctg acctgtaggg tggataaggc gttcacgccg catccggtat cggaactgac
     1981 agttggtgaa cgcattatct acgttctatt tacagcagca cctccagccc gtgtttttgc
     2041 acaatgctga gcagttttaa tgaaatgcca ttggggcgtt tagcgccact ttcccatttt
     2101 tgtacagttg aaacgctggt attgagatag tgagcgaaga cgggctgact cacttttaat
     2161 ttttcccgca gtgcttttat ctcaacaggt tgtaagtctt gcacgctggt caggcaggct
     2221 ttgtcaaaag tacgcatcgt ttcggcagaa atcgcatcga cgctggacaa tccagccgct
     2281 gcgctgtgaa tagcctcaaa tgcaggactt ttaaatttag tttttgcagt catagcagat
     2341 ctctgtcagt tctttagtgt taatcattgc tgtcagttgg gctttcgtca ggaaggcata
     2401 atgctttgca agttcacgaa acccagccag ttcctgacta ttaatgttcg acatatcctg
     2461 ttttgcatat aaaaacgtgt aaaaccaatt gattccccct ttcgccagaa taattgcacg
     2521 ctcacgattt tggtgaaggc gctttttaaa aacgccaccg ccaagtgcat cagctttacc
     2581 ttgttccgtt tctaaaatgg cccggcacaa ctcttcatct gttattgcat gagacctggc
     2641 ttcccttgca aaccatttgg ttttaaacac gcgcatatcg ttgagatcct gttattaaag
     2701 tatatcacca agtgcaatac tttatccctt gttcccaaca caatcgcgaa aatacatatc
     2761 accttgccct atgattttta tttcttcgag cagcctcgaa ctttcccaca acaccgtaaa
     2821 agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg cttttcccct
     2881 gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg gcgcggatgt
     2941 catcacccga ccgaactggt cagccgtttt ctcggtggcg ttttgcgtcg cctgtctgat
     3001 tatcgttgag tttttgcccg tcagtttgtt gacgccaatg gcccaggatt taggcatttc
     3061 ggagggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt ttgccagttt
     3121 gtttattacc cagacaattc aggccactga ccgccgctac gttgttattt tgtttgccgt
     3181 tttgctgacg ctctcctgtt tgctggtttc ctttgccaac tcattcagtt tgcttttaat
     3241 cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg cgtcgctgac
     3301 catgcgtctg gtaccgccgc gtacggtgcc gaaggcgctg tcggtgatct tcggcgcggt
     3361 ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc ttatcggttg
     3421 gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgctg tgtattttct ggattatcaa
     3481 atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt tccgcttatt
     3541 acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg ccgggcagtt
     3601 tgctttcttc acgtatattc gcccggtgta tatgaacctg gcgggattcg gcgtggatgg
     3661 cttaacgctg gtgctgttga gttttggcat tgccagcttt attggtacgt cgctttcgtc
     3721 gttcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa tactggctgt
     3781 gagtgcgttg gtgctgacgt tgtggggaag cgataaaatc gttgctaccg gcgtggcaat
     3841 tatctggggg ctaacttttg cattggttcc ggtcggctgg tcaacgtgga tcacccgctc
     3901 actggccgat caggcagaaa aagccgggtc aattcaggtg gcggttattc agcttgctaa
     3961 tacctgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     4021 gatgttgtcc ggcacattga tgttgctgac ggcgttattg gtcaccgcaa aggtgaaaat
     4081 gaagtaacgt atttacagga ggggttactg ctctggcggg aagctctcct gttcttcccc
     4141 tgataaccat tgatcaggta ttgttcttat cttgttgtta tttccattat tccgcctgta
     4201 ttcatcattt gctaatcatg aatcatcatt ttctctcatg ttaaagaaag gtcatttaat
     4261 ctgaacgcat agcgacaggg atgacaaatg gcagggaaag cgcatgggaa tggcgatcgt
     4321 cggggcgata acaccatatg tggattgggg gatcgtttac gtcgtctgac cgcaggtatt
     4381 tgcctgataa cacaaactat tttccctgtt atggctgctg cccctacgca tattaatcct
     4441 gcgcactcgg atactgccgc gtcactgatc ctgccgaacg ttaaaactat tccatatacc
     4501 ctgggtgcgt tggaatcgcc accaacggtc gcggcacgct ttggtatcac cgttgatgaa
     4561 ttacgtcgcc tgaatcagtt ccgtaccttt gcgcggggct ttgacaatgt gcgtcagggc
     4621 gatgaaatag atgtgcccct cattaacagt aatagccccg aagcgcgcaa cctgaaagca
     4681 atgcagatgg agcgcgacgg caaagatccg caaatgcagg tcgcggaaat ggcgcagcaa
     4741 agcggaacac ttttagctcg cgatatggac agcgaacagg cggcgagtat ggcgcgtggc
     4801 tgggtggctt cttctgcatc tgcgcaggca acggactggc tcagtcgttg gggaactgcc
     4861 cgggtatctc ttggtgtgga tgaagatttc agccttaaaa gctcgtcatt tgaatttctc
     4921 catccctggt acgaaacgcc ggataacctg gtcttcagcc agcacacact tcaccgtacg
     4981 gataatcgca ctcaaaccaa ccacggtatc ggctggcgtt atttcacatc gagctggatg
     5041 tctggcgtca atatgtttat cgatcatgac ctgacccgct atcacacccg aaccgggatg
     5101 ggcgttgaat actggcgcga ttacctgaaa ctgagcggca acggttatct gcgcctgagt
     5161 aactggcgta gtgcaccgga actcgacaat gactatgaag cacgtccggc gaacggttgg
     5221 gatcttcgcg ccgaaggctg gttaccggcc tggccgcagc tgggcggcaa ggtggtctat
     5281 gaacaatatt atggcgatga agtggcgctg tttggtaaag acgagcgtca aaatgatcct
     5341 cacgcgatta ccgcaggttt gagttatacc cctgttcctc tgatctcctt tagtgccgag
     5401 caacggcagg gcaaacaggg agaaaacgac acccgaattg gtatggagtt gacgttgcaa
     5461 cccggccatt cactgcaaaa acagctcgat ccggctgaag ttgcagcacg acgtagcctt
     5521 gtgggcagtc gctatgacct ggtcgatcgt aataacaata ttgtcctcga atatcgcaag
     5581 aaagagctgg tgcggctgac gctgaccgat ccgttgaaag gcaagccggg tgaggttaaa
     5641 tcgctggttt cttccttaca aaccaaatat gcgctgaaag gttatgacat tgaagccgcc
     5701 tccctgcaat cagcgggcgg aaaagtagct gtttccggca aggatattca ggtcactatt
     5761 cctccctacc gattcacggc aatgcctgaa accgacaaca cttacccgat agcggttaca
     5821 gcggaagatt cgaaaggaaa tttctcccgg cgtgaagaaa gtatggtggt cgtggaaaaa
     5881 cccacattaa gcctcgccgg ttcaacgctt tctgtcgatc tgcaaatcct gcttgctgat
     5941 ggaaaatcaa cctcaacgct gacctatacc gcgcgagatt ccagcggcaa gccaatccca
     6001 ggtatgacgt taaaaacaca ggtgaaaggt ttgcaggatt tcgccttaag cgaatggaaa
     6061 gacaacggta acggaaccta tacccaaata gttaccgccg ggaagacgtc tggcgcgtta
     6121 tcgctgatgc cgcaatttaa cggcgatgat atcgctaaga cgccagcact gattgctatc
     6181 gtggcgaata cagcatcccg cgctgactcc acgattgaga cagaccagga taattacgtc
     6241 gcgggtaagc cgatagtcgt taaagtgacc ctgagagatg ataacggcaa cggggttacc
     6301 ggacgaaaag aacttctcaa acaaaccgtt aaagttgaca acacgaaggc tgatgccgtt
     6361 agtgcctgga cggaagaaag tgagggaatt tacaaagcaa gctatacggc ccatctcatt
     6421 ggcgacaaat taacggccca gctgacgatg cccggctggc aaaccaaaca ttcggatgcg
     6481 ttcagtattg cgggtgataa agataccgcc aaaatcgctg cgatgcagat caccgcgaat
     6541 aacgcagttg ccagacgcga tcacaatacc gtcgctgtga cggtcaggga tgtgcatcaa
     6601 aatttactgc agggacaaaa tgttaccttc acggtcgtga atggtgcggc ggtttttgcc
     6661 gatccaaatg gcggtattgt gacgacagat aaagacggga ttgccagcgt taaccttgcc
     6721 agtgaccagg cggtaaacag tcttatcaag gccgagacca atggcagtag tcagtctgtg
     6781 gaagtgagct ttattactgg cgatatctct cagctgacct ccaccatcaa gacagacgat
     6841 gtgtcataca ccgcgggtgg caagataaaa gtgtcagtta cccttatgga tgagcagaaa
     6901 aacctggtga aagggatggc ttcgctgctg gcgggtagcg gtgtggtcga ggtgagcggt
     6961 acagataaaa atgaaacggg taactggagc gaagagtctg atggcgttta caccaccacc
     7021 cgaaccgcga agattgctgg cgatcgccat tacgccacgc tgaaactatc gacatggagt
     7081 agcgcgcagc aatccgatgc gtatgccatc cgcgaaagcg gagctgtgtt ggcttattcc
     7141 tccatcgtta ccgacaaaac gacctacacc gcgggcggcg cgataaaagt tacggtaaca
     7201 ctcaaggata gctatgaaaa cctggtcggg gggcaacgag acgctatcaa cctggctatc
     7261 cagctgccaa atacaaaagc ggaaagcatc gcctggaatg aagatcaaaa aggaatttat
     7321 accgcaacgt ataccgcctt actccctggc accggtttaa aagcacagct acagatgtcc
     7381 ggctgggcga acgctctgac ttcaaatgat tacagtatta gtggcgatgc cgccagcgca
     7441 caaattgtcg cgatgcaagt gacaacgggt aacccggatg tgctggcgaa tggcagcgat
     7501 cgacatacag tgaatgtccg ggtggaagat cagtttggca atgtgctgcc ggagcagacc
     7561 gtcaccttta cggtgacgaa gggggcggct gtatttgcca atgcagggca gagcgcaggt
     7621 atcaggacgg atgcgcatgg catggcagag gttgatctga gcagtaccgt agcggatgcc
     7681 agcactgttg aggctaagat caatcaaagc agcgatagta aaacggtgaa ctttgttgct
     7741 gatgttagca cggcgcaggt cgcggagctg gtggtcatta aggatggttc agaggcggac
     7801 ggttcgacgg cgaacacgct gcgggtgaag gtcaccgatg cgttcggtaa tacgcttgcc
     7861 gggcagacgg tcagcgtgtt ggcggacaac ggtgcaacgg tcgctccgac ggtgattaca
     7921 gagccggacg gcacggtgga gatctcagtc accagccaga ccgccggaac cagtgtggtc
     7981 accgccagcg tcaacaacag cagcc
//
LOCUS       DAOTRK010000187         7900 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.188, whole genome shotgun sequence.
ACCESSION   DAOTRK010000187 DAOTRK010000000
VERSION     DAOTRK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.188"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(85..963)
                     /gene="glxR"
                     /locus_tag="QIE41_004229"
     CDS             complement(85..963)
                     /gene="glxR"
                     /locus_tag="QIE41_004229"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDS3533032.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1063..1839)
                     /gene="hyi"
                     /locus_tag="QIE41_004230"
     CDS             complement(1063..1839)
                     /gene="hyi"
                     /locus_tag="QIE41_004230"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDS3533033.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1852..3633)
                     /gene="gcl"
                     /locus_tag="QIE41_004231"
     CDS             complement(1852..3633)
                     /gene="gcl"
                     /locus_tag="QIE41_004231"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDS3533034.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            complement(3723..4538)
                     /gene="allR"
                     /locus_tag="QIE41_004232"
     CDS             complement(3723..4538)
                     /gene="allR"
                     /locus_tag="QIE41_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDS3533035.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4616..5098)
                     /gene="allA"
                     /locus_tag="QIE41_004233"
     CDS             complement(4616..5098)
                     /gene="allA"
                     /locus_tag="QIE41_004233"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDS3533036.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5328..6254
                     /gene="allS"
                     /locus_tag="QIE41_004234"
     CDS             5328..6254
                     /gene="allS"
                     /locus_tag="QIE41_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDS3533037.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YTPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6323..7417
                     /gene="mnmH"
                     /locus_tag="QIE41_004235"
     CDS             6323..7417
                     /gene="mnmH"
                     /locus_tag="QIE41_004235"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDS3533038.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELSQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
BASE COUNT         2018 a         2072 c         1905 g         1905 t
ORIGIN      
        1 actgaagcaa catctttggc tgctttcttt tttaatattg ctggccgatt cattttctga
       61 tatcggccat ttttattgtg ggtatcaggc cagtttatgg ttagccatta attccagcgc
      121 ctgcactaac gcagagtgat ccaactggct gccaccgttt gccgcacagg tattaaataa
      181 ctcctggcag gtcgcagtgt ttggcaggtt cagcgcaagt gctttcgcac tttgcagtgc
      241 caggttgaga tctttctggt gcagcgcaat tttaaaacct ggattaaagg tgcgtttaat
      301 catgcgctca ccgtgaactt ccagaatacg tgaggaagca aagccgccca tcagcgcctg
      361 gcgcacacgt accggatccg ctccggcttt tgaagcaaat agcagggctt cagaaaccgc
      421 ttcaatattg agcgccacga taatctgatt tgccactttg caggtttgac cgtcgccgtt
      481 accgcccacg agggtgatat ttttaccgag cagttcaaac agcggtttaa cacgttcaaa
      541 taccgcttcg tcaccgccaa ccataatcga caacgtccct tcacgcgcgc cgatttcacc
      601 gccggagacg ggcgcatcga gataatcacc gcccagttca ttcacctgac gagcgaaacg
      661 cttagtttcg atcggggaaa tggagctcat atcaacaatg gttttgccct tcagcgaggc
      721 tttggtacaa ccattttcac cgaacagaac ttcttcaacc tgtggtgtat ccggcaccat
      781 aataaaaatg atgtccgatg cttccgttac ctggcgcgca gtttcaacac tgacggcacc
      841 cagtgacagt aattcatcag caaccggacc aatggttgtg acatgcagtt gatgaccggc
      901 acgcgccaga ttaatggcca tcggtgtacc cataatgcct aagccaataa atcccagttt
      961 cataattaac ctcttttaaa tttcgctttt cctgaattca gacaacacga tcccctgcca
     1021 acttagcggc ctaaaaaagc attgtctgaa tagcgttacg ttttaacggt acggatccat
     1081 ccagcgtaaa ccggcttccg tggtagtttg gggtttatat tcacacccaa cccaaccgtt
     1141 gtagtcagaa ttttcgatta ccttaaagag ataatcataa ttaatttctc cggtccccgg
     1201 ttccccgcga tgcggattat cggcaatttg caggtgacca attttatcgg cccattgcgt
     1261 catagtgttg gttaattcac cttccatccg ctgcatgtga taaatgtcat actgaatttt
     1321 caaattgcag caaccaacat catcaatcag tttcagtgcc tggcgagttc cggtgagatg
     1381 gaaaccagga atatcaaaat ggttgatagg ttcaatcagt aataaaatat cttctttcat
     1441 cagcatattc gcggcataac gcaggttttc tacaagcgtt gtgtgaatct gttcactgct
     1501 gaaaccagcc ggcgttttac cgaccagaca gttaattttt ttattaccca gcgcacgagc
     1561 ataacgaatc gctgctgcta cgccatcccg aaactcttct tcacgaccag gaatacaagc
     1621 aataccccgc tcgcccgccg cccagtcacc tgccggtaaa ttgtgcaacg tatgttcgag
     1681 tttattactc gccagcacct gttttaattc ttcaatgtca tagtcataag ggaacataaa
     1741 ttcaacgccg cgaaaaccac actgcgcagc tttctcaaaa cgggcgagaa aatcatattc
     1801 accaaataac atcgataaat tagcagagaa acgtaacatt atttatctcc cttattcata
     1861 gtgcatgaag caggtttcag tcggtgcgtc cactgcgtta tcggcgatat cttcaaattc
     1921 catgacgtta tccagttcgc tgcccatcga aatattggtc acacgctcga gaataacttc
     1981 cacgactacc ggtacccgat attgcgccat taaggctttc gcctgttcaa aggctggtgc
     2041 aatatcttcc ggtttgaaga cccgaatcgc tttacaacct aaaccttccg ctacttttac
     2101 gtggtcgacg ccgtacccgt tcacttcact ggagttaata ttttcgaacg cgagctgtac
     2161 gcagtaatcc atatcaaacg cgcgctgcga ctggcgaatc agacccagat aggcgttatt
     2221 gaccagtaca tggatgtacg gaatgttgaa ctgtgcgcca acggctaatt cttcaatcag
     2281 aaactggaag tcgaagtcac cggaaatcgc caccactttg cgctctggat cagcggcaca
     2341 aacccccagc gccgccggaa tggtccagcc cagcgggcct gcctgcccac agttgatcca
     2401 gtggcggtct ttaaagacat gcagcatttg tgccgcagcg atttgtgaca ggccaatggt
     2461 ggtgacataa caaacatcgc gaccaaaggc tttgttcatc tcttcataca cgcgctgcgg
     2521 tttcaccggc acgttgtcga agtgggtttt gcgcagcaaa gtgcgtttgc gctgctggca
     2581 gtcggcgacc cattctttac gacacggcag acgacccgct ttttgcatct cctgcgccac
     2641 ttcaaccagc agcgtcagcg ccgctttagc atcagagaca atgccgagat ccggacacag
     2701 cacgcggcca atttgcgtcg gctcaatatc gatatgaacg attttgcgcc cttcggtgta
     2761 tttctctacc gaaccggtat gacggttcgc aaaacggtta ccgataccaa acaccatgtc
     2821 ggacgccagt agcgttgcgt taccgtaacg atgcgcggtt tgcagaccca ccatcccggc
     2881 catcagttca tgatcgtcgg gaatacagcc ccagcccatc agcgttggga tcaccggaac
     2941 gctggtcagt tcagcaaact gttgtaacag tgcagcagcg tcagcattga ttaccccgcc
     3001 cccggcaaca atcaccggac gttcggcctg aattaacatt tctaccgctt tttcgatctg
     3061 catacggctg gcagcaggtt tgtagaccgg cagcggttcg tacatgtcag gatcaaactc
     3121 gatttccgca acctgaacgt cgaacggtaa atccaccagt accggacccg ggcgaccaga
     3181 acgcatcagg tgaaacgcct gttgcagcac acgaggcacc agcgccgctt cacgaactgt
     3241 aaccgccatt ttgctgaccg gtttagcaat tgcttcaata tcaacggcct ggaaatcttc
     3301 tttatgcaga cgggcgcgcg gagcctggcc ggtaatacac agaataggaa tggaatcagc
     3361 agaagcggaa tagagcgcgg tgatcatgtc cgtgcccgca ggaccggaag tccccagaca
     3421 tacgccgata ttccctgccg ttgcgcgggt ataaccttcc gccatgtgcg aagcaccttc
     3481 cacatgacgc gccagaatgt gacgaatacc gccgtgctta cgcatcgctg aatagaacgg
     3541 attgattgct gctcccggaa cgccgaaggc ggtggtgata ccttctttct ccagcacata
     3601 cattgccgcg tcaacggctc tcatttttgc cattttattc ctacctctat aaattggaaa
     3661 aattttccaa ctttcaaatt ttgtcaaaaa aacccgctcc gcagagcggg atgcgacaga
     3721 cactatggat gtgctttcag tcccaacgcc gtgctgatat cgcgggcggt gtctctgacc
     3781 agctcaccct gactgacaaa acgatcttct gtcagtcttg atgaaggccc ggagatggag
     3841 atagcggcaa caacgctacc tacatcatcg taaattgctg aagctatgca attcagacct
     3901 acaacatgct cttctttatc tacggtatag cccagttcac gcgcttgttc caggtccttc
     3961 agcaaggtgg gcatatccac aagcgtagtt ggcgtaaact gctgcaaacc ggtttgcaga
     4021 atgatgctca tcaactcctc ttccgccagc ggataaagca acgccttgcc cgcaccggaa
     4081 gcatgcagcg gcagacgact gcccagtggc gcacacatcc tgaccatcga tttacactct
     4141 aactgaccaa ttaatactgc ttcattgccg ttacgaatcg cgacattgac cgtttcgccg
     4201 gaaagtaaca tcaggcggcg cataaacggc ccggcgacgg agaggacatc gcggttatgg
     4261 atgtacgccg caccgacatt aaagacacct aatcctatat gccaccagcc taactgactg
     4321 tcctgataga caaaatccgc tgcctgtaaa accttcagca agcgaaaggt cgtggagagc
     4381 ggcaaatcca gattgagaga aatatcgcta accgatgaac ttcccccact tttttccaga
     4441 tattgcagaa tcgcaattcc ccgctctaac gcctgtgcgc ccttctgtgc cacaggctcc
     4501 gcctgtcctg gcctgccgcg ccgtctaact tccgtcatac ctacgttaac tccgacctac
     4561 aagtgagcat aaacaatgaa caattagttt atcttatgca agtcggttgc aggcgtcacg
     4621 caaaacagag ttcctgttca ggaatacttt caacatcaca gttgtcactg ccgccgcgat
     4681 cgatggtcag aaaatcggtg acgcgctgcc aggcgaaaag tgggtgatgc cagacattac
     4741 gatggtaatt caccccctgt tcaccgttgg tgataaacgc ccgcagcgtt gacaggtctg
     4801 gtttgtcgtc acctaacgcc acgaccacca caaacacttc acctttcatc gggataaagg
     4861 cctgagtgcc cagcggatga cgttcgagtt cgtgaatggt cagcggcaga ttcgccggtt
     4921 gcgcgcggtt aatgctgatg agcgtacggt cttgctcaag aatctcaacc agcgccaaat
     4981 cgtggtaacg ctctaccaga ccattgttaa tatggaaaaa atcccgttgc tgcgtttcga
     5041 ttacatcgcc ataagcacta aaggcttcct gacttaacgg taatacctga agtttcatct
     5101 ttttgtcctt gtaacataat tccgttcaac caccgccgac cggtcagctt ggtttcaata
     5161 acaggcaatc taaaacctta agagaacaac atcaacacaa aacttggaat atttttccaa
     5221 aactagattt agtccacaaa ttgaacaggg ttatcatgta aaaatactga taaaccagag
     5281 gataagaaaa tcacgcaact tgctgattta aaaggggata cctgaagatg ttcgatccag
     5341 aaaccttgcg gactttcatt gcggttgctg aaacaggaag tttttcaaaa gcggcagaac
     5401 gattgtgtaa aaccacggcg acgatcagtt atcgcattaa acttctggaa gagaataccg
     5461 gagtggcgct gtttttccgc acgactcgca gcgtgacgtt gacagcggct ggcgagcatc
     5521 tactttctca ggccagagac tggctgagct ggctggaaag tatgcccagc gagctgcaac
     5581 aggtgaatga tggcgtggaa cgccaggtga atattgtcat caacaacctg ctctacaccc
     5641 cccaggccgt cgcccagctg ctggcgtggc tgaatgaacg ttacccgttt acccagtttc
     5701 acatctcccg acaaatctat atgggcgtct gggactcgct attgtacgaa ggtttttcac
     5761 tggctatcgg cgtcacggga accgaggcgc tggcaaatac ctttagtctt gatcccttag
     5821 gatcggtgca atggcgcttt gtcatggcgg cggatcatcc gctggcgaac gttgaagagc
     5881 cgctaacaga agcacagttg cggcgctttc cggcggtcaa tattgaagac agcgcccgaa
     5941 cactaaccaa acgcgtcgcc tggcgattgc cggggcaaaa agagattatt gttcccgata
     6001 tggaaacgaa aatcgccgcc catctggcgg gcgttggcat tggttttttg ccaaaatcac
     6061 tttgccagtc aatgatcgat aatcaacaac tggtcagccg ggtaatccca acgatgcgcc
     6121 ctccttcgcc attgagtctg gcatggcgca aatttggcag cggcaaagcg gtagaagata
     6181 ttgtgacctt gtttacccag cgcaggccag aaatcagcgg atttttagaa attttcggca
     6241 acccacgcag ttaaaaatca agtttccttg cgcgttggcg cgtaaaatag cgcgcaagct
     6301 gatttgaggt tcacttttaa ctatgcaaga gagacacacg gaacaggact atcgtgccct
     6361 gctgattgct gatacgccca ttattgatgt tcgtgcccct atagagtttg agcaaggcgc
     6421 aatgcccgcc gctatcaatc tgccgttaat gaataacgat gaacgcgccg ccgttggaac
     6481 ctgctataaa cagcaagggt cagacgcggc gctggcgctg gggcataaac tggtggcggg
     6541 tgaaattcgt cagcagcgta tagacgcctg gcgggcagcg tgcctgcaaa atccgcaagg
     6601 tattctctgc tgcgcccgtg gcggtcagcg ctcacatatt gtgcaaagct ggttgcatgc
     6661 agcggggatt gattatccgc tggtagaagg cggttataag gcactgcgcc agaccgcgat
     6721 tcaggcgacc attgaactgt cacaaaaacc gatagtgctg attggcggtt gtaccggcag
     6781 cggtaaaacg ctattagtgc aacaacagcc aaacggtgtt gatctggaag ggctggcgcg
     6841 tcatcgcggt tcggcgtttg gtcgcacgtt acaaccacaa cttagccagg caagttttga
     6901 aaacctgctg gctgccgaaa tgctaaaaac cgacgcccgt cagaatttgc gcctgtgggt
     6961 gctggaagat gaaagccgga tgatcggttc gaatcacctg ccggaatgtc tgcgcgaacg
     7021 aatgacccag gcggcgattg cggtggtaga agatccgttt gagatccgtc ttgagcgcct
     7081 gaacgaagag tatttcttgc gtatgcatca tgattttacc cacgcgtacg gcgacgaaca
     7141 gggctggcag gaatattgcg aatacctgca tcacggactt tcggcgatta agcgtcggct
     7201 ggggttacag cgatataacg aactggctgc aaggctggat gctgcgctga caacgcaact
     7261 caccaccggc agcaccgacg gtcatctggc ctggctggtg ccgttacttg aagaatatta
     7321 cgacccgatg tatcgctatc agcttgagaa aaaagctgaa aaagttgtct ttcgcggtga
     7381 gtgggcggaa gtggcggaat gggttaaggc gcagtgatgg gcggtattct gaaacggcga
     7441 ttgtcgtgag aacagaatgg ttgcaccaga atacagatat aaaaaaaccg acatgacagt
     7501 cggttttttt attttttccg cccaagactt ggtggttccg ggcagaaatg aaacagtcct
     7561 catctagcaa gtgaattata agtcaggatg cctacatatt caaacagcta tgttggcgat
     7621 acaatcccga cgctagtagt gatactgcct caagggatta ccgcagtgcg gaaaagtact
     7681 tttaagtcct ggtcaggata caggtggtgc tgattctggt gctctaattt ggagagaggc
     7741 agtcctcatc tagcacatga agaaaatcat agttacaggc aacgtaaggg taagctgtga
     7801 ttgtctttct cccttaaggg agcaacggat gaaaactttt ctcgataaag ttctcatcgt
     7861 tcttaaggcg ctaatcgcgc tgctggaact gattcgtcag
//
LOCUS       DAOTRK010000188         7896 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.189, whole genome shotgun sequence.
ACCESSION   DAOTRK010000188 DAOTRK010000000
VERSION     DAOTRK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.189"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            159..518
                     /locus_tag="QIE41_004236"
     CDS             159..518
                     /locus_tag="QIE41_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDS3533039.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERIVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(638..817)
                     /locus_tag="QIE41_004237"
     CDS             complement(638..817)
                     /locus_tag="QIE41_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDS3533040.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            891..2252
                     /gene="pabB"
                     /locus_tag="QIE41_004238"
     CDS             891..2252
                     /gene="pabB"
                     /locus_tag="QIE41_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDS3533041.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQHQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSVKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2256..2834
                     /locus_tag="QIE41_004239"
     CDS             2256..2834
                     /locus_tag="QIE41_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDS3533042.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3018..4382
                     /gene="sdaA"
                     /locus_tag="QIE41_004240"
     CDS             3018..4382
                     /gene="sdaA"
                     /locus_tag="QIE41_004240"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDS3533043.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4513..6111
                     /locus_tag="QIE41_004241"
     CDS             4513..6111
                     /locus_tag="QIE41_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDS3533044.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6115..7671)
                     /gene="yoaE"
                     /locus_tag="QIE41_004242"
     CDS             complement(6115..7671)
                     /gene="yoaE"
                     /locus_tag="QIE41_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDS3533045.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPVHDEDE"
BASE COUNT         1941 a         2053 c         1990 g         1912 t
ORIGIN      
        1 ggttttcttt cttagctgaa caatctggtt atagcgtgcg gactgaccgt taattttctc
       61 cctcttactg gataacacac catgagctgc tacctttaaa acacctgaat gtcaacaggt
      121 taactcgtgc catatcgtta taatcccttt gatattagat gcaaattaag gtcatatata
      181 gccttatcga taacatggtt aattttaagg ataagaatat gcctgctgta atagataaag
      241 ccctggattt cattggtgcc atggatgtat cagcaccaac accaagttcg atgaatgaaa
      301 gcacggcgaa gggaatcttt aaatatttaa aagaactggg agtacccgcg agcgcagccg
      361 atattacggc gcgagctgac caggaaggct ggaacccggg gttcacggaa aaaatggttg
      421 gatgggcaaa aaaaatggag tctggtgaac gcattgtgat taaaaatcct gaatactttt
      481 caacatatat gcaggaagaa ctaaaagcac tggtctaagt taaatctaca tcagcataaa
      541 tggatcagga cgcttttaat cacatatgaa aagcgtcctt tttctcacaa ccaaataacc
      601 attgacttct ataacgaaag cgatacggat aagtgtgtta ctcgtcttca tcctcaaaac
      661 gcgccacgat tcgctcgccg gaatggttgg cacgcagctc ttctgccacc aacgcgattg
      721 cctggccgct gctcatccct tgtgccatca gttcctggat ccgctcgacg gctttttgct
      781 gctgttcatg ggtgagtgaa ggtaaacctg caaacattgt taactcctgc taaattgttg
      841 gcgctaatta tttcatgcta cccggcacat agccagtaga gtcaggactg atgaagacgt
      901 tatctcccgc tgtgattact ttaccctggc gtcaggacgc cgctgaattt tatttctcac
      961 gcttaagcca cctgccgtgg gcgatgcttt tacactccgg ctatgccgat catccgtata
     1021 gccgctttga tattgtggtc gccgagccga tttgcacttt aaccactttc ggtaaagaaa
     1081 ccgttgttag tgaaagcgaa aaacgcacaa cgaccactga tgacccgcta caggtgctcc
     1141 agcaggtgct ggatcgcgca gacattcgcc cagcgcataa cgaagatttg ccatttcagg
     1201 gcggcgcgct ggggttgttt ggctacgatc tgggccgccg ttttgagtca ctgccagaaa
     1261 ttgcgcagca agatatcgtt ctgccggata tggctgtggg tatctacgac tgggcgctgg
     1321 ttgttgacca ccagcatcaa acagtttctt tgctgagtca taatgatgtc aatgctcgtc
     1381 gggcctggct ggaaagccag caattctcgc cgcaggaaga tttcacgctc acttccgact
     1441 ggcaatccaa tatgacccgc gagcagtacg gcgaaaaatt tcgccaggta caggaatatc
     1501 tgcacagcgg tgattgctat caggtgaatc tcgcccagcg ttttcatgcg acctattctg
     1561 gcgatgaatg gcaggcattc cttcagctta atcaggccaa ccgcgcgccg tttagcgctt
     1621 ttttacgcct tgaacagggc gcgattttaa gcctttcacc agagcggttt atcctttgtg
     1681 ataatagtga aatccagacc cgcccgatta aaggcacgct accacgcctg cccgatcctc
     1741 aggaagatag caaacaagca gagaaactgg cgaactcagt gaaagatcgt gccgaaaatc
     1801 tgatgattgt cgatttaatg cgtaatgata tcggtcgtgt tgccgtagca ggttcggtaa
     1861 aagtaccaga gctgttcgtg gtcgaaccct tccctgccgt gcatcatctg gtcagcacca
     1921 taacggcgcg gctaccagaa cagttacacg ccagcgatct gctgcgcgca gcttttcctg
     1981 gtggctcaat aaccggggct ccgaaagtac gggctatgga aattatcgac gaactggaac
     2041 cgcagcgacg caatgcctgg tgcggcagca ttggctattt gagcttttgc ggcaacatgg
     2101 ataccagcat tactatccgc acgctgactg ccattaacgg gcaaatatac tgctctgcgg
     2161 gcggtggaat tgtcgccgat agccaggaag aagcggaata tcaggaaact tttgataaag
     2221 ttaataagat attacgccaa ctggagaagt aagacgtgga ataccgtagc ctgacgcttg
     2281 atgatttttt atcgcgcttt caacttttgc gcccgcaaat taaccgggaa accctaaatc
     2341 atcgtcaggc tgccgtgtta atccccatcg tccgtcgacc gcaaccgggg ttgttgctga
     2401 ctcagcgttc gattcatctg cgtaaacacg ctggacaagt ggcattccct ggaggcgcag
     2461 tcgatgacac ggacgcatca gttatcgccg ccgcgctgcg cgaagctgaa gaagaggtcg
     2521 ctataccgcc ttctgccgtt gaagttattg gcgtgctgcc gcccgtcgat agcgtaactg
     2581 gctaccaggt aaccccggtg gtcggcatta tcccgcccga tctgccgtat cgcgccagtg
     2641 aagatgaagt ctcggcggtg tttgaaatgc cgctcgccca ggcattacat ctgggtcgtt
     2701 atcacccttt agatatctac cgccgtggcg attcacatcg ggtatggctg tcctggtatg
     2761 aacagtattt tgtatgggga atgaccgcag gcataattcg tgagctggcg ctgcaaattg
     2821 gtgtgaaacc ctgactatac ttatctttac atctacaaaa cactacttga gacaatcatc
     2881 gcaatattag ttaaatcgcg gtttttgatt agtttaattc atgtgaatag ttaagccagt
     2941 cgccgcgttc cctcttacac tatgcgctgt tattagttcg ttactggaag tccagtcacc
     3001 ttgtcaggag tattatcgtg attagtctat tcgacatgtt taaggtgggg attggtccct
     3061 catcttccca taccgtaggg cctatgaagg cgggtaaaca gttcgtcgat gatctggtcg
     3121 aaaaaggctt actggatagc gttactcgcg ttgccgtgga cgtttatggt tcactgtcgc
     3181 tgacgggtaa aggccaccac accgatatcg ccattattat gggtcttgca ggtaacgaac
     3241 ctgccaccgt ggatatcgac agtattcccg gttttattcg cgacgtagaa gagcgcgaac
     3301 gtctgctgct ggcacaggga cggcatgaag tggatttccc gcgcgacaac gggatgcgtt
     3361 ttcataacgg caacctgccg ctgcatgaaa acggtatgca aatccacgcc tataacggcg
     3421 atgaagtcgt ctacagcaaa acttattatt ccatcggcgg cggttttatc gtcgatgaag
     3481 aacactttgg tcaggatgcc gccaacgaag tgagcgtgcc gtatccgttc aaatctgcca
     3541 ccgaactgct cgcgtactgc aatgaaaccg gctattcgct gtctggtctc gctatgcaga
     3601 atgaactggc gctgcacagc aagaaagaga tcgacgagta tttcgcgcat gtctggcaaa
     3661 ccatgcaggc atgtatcgat cgcgggatga acaccgaagg cgtactgcca ggcccgctgc
     3721 gcgtgccgcg tcgtgcgtct gccctgcgcc ggatgctggt ttccagcgat aaactgtcta
     3781 acgatccgat gaatgtcatt gactgggtaa acatgtttgc gctggcagtt aacgaagaga
     3841 acgccgccgg tggtcgcgtg gtaactgcgc caaccaacgg tgcttgtggc attgttccgg
     3901 cagtgctggc ttactatgac cactttattg agtccgtcag cccggacatc tatacccgct
     3961 actttatggc agcgggtgcg attggtgcac tgtataaaat gaacgcctct atttccggtg
     4021 cggaagtcgg ttgccagggc gaagtgggtg ttgcctgttc aatggctgct gcgggtcttg
     4081 cagaactgct gggcggtagc ccggaacagg tttgcgtggc ggcggaaatt ggcatggaac
     4141 acaaccttgg tttaacctgc gacccggttg cagggcaggt tcaggtgccg tgcattgagc
     4201 gtaatgccat tgcctctgtg aaagcgatca acgccgcacg catggcctta cgccgcacca
     4261 gtgcaccgcg cgtctcgctg gataaggtca tcgaaacgat gtacgaaacc ggtaaggaca
     4321 tgaacgctaa ataccgcgaa acctcacgcg gtggtctggc aatcaaagtc cagtgtgact
     4381 aatacttctt actcgcccat ctgcaacgga tgggcgaatt tatccccgct ttctcgtctg
     4441 ctgtaatttt ccccactaca cttccactgt tgcgtcaggc gtttgtcgcc atacgcttac
     4501 agggtggccc gcatgcaaaa agcacaacgg atcattaaaa cctatcgccg taatcgaatg
     4561 attgtttgta cgatttgcgc actcgttacg ctcgcttcga ccctgagcgt gcgatttatt
     4621 tcacagcgta acttaaatca acaacgggta gtacaattcg ccaatcacgc tgtagaggaa
     4681 ttagataaag tactgcttcc cctacaggca ggtagcgaag tcttgcttcc gctgattggt
     4741 ctgccctgct ctgtcgccca tttgccatta cgtaaacagg cggcaaaact ccaaactgtg
     4801 cgatccattg gcctggtgca agacggcaca ctttattgct ccagcatttt tggttatcgc
     4861 aatgtgcccg tcgtggacat tctggctgaa cttcctgcac cgcaaccact tttacgcctg
     4921 acgaccgacc gtgtcctgat taaaggcagt ccggttttga ttcaatggac gccagcagcg
     4981 ggcagtagca aagctggggt catggagatg attaacatcg acttactggc ggcaatgctg
     5041 cttgagccac aactgccgca aatcagtagc gccagcctga cggtggacga tcggcatttg
     5101 ctctatggta atgggctggt agattccctt ccgcaacctg aaaacaatga aaactatcag
     5161 gtttcttcgc aacgctttcc ttttaccatt aacgttaatg gtccgggggc tacggcgctg
     5221 gcatggcact atcttccaac acaattaccg ctggcggtgc tgctaagttt acttgtgggc
     5281 tatatcgcct ggctggcgac cgcttaccgg atgagttttt cccgtgaaat caatctgggc
     5341 ctggcgcaac atgagttcga attgttctgc cagcctttgc ttaatgcgcg cagccagcaa
     5401 tgtattggcg tagagatttt gctgcgctgg aacaatccac gtcagggctg gatttcaccg
     5461 gatgtgttta ttcctatcgc ggaagaacat catttaattg tgccactgac ccgctatgtg
     5521 atggcagaaa ccattcgtca gcgccatgtt ttcccgatga gtagtcagtt tcatgttggc
     5581 attaacgtcg cacccagcca ttttcgccgt ggtgtgctga taaaagatct caatcagtac
     5641 tggtttagcg ctcacccgat tcagcaactg atcctcgaaa tcaccgaacg cgatgcctta
     5701 ctggatgttg attatcggat tgcccgcgag ctacatcgta aaaacgtcaa actggcgatt
     5761 gatgacttcg gcaccggcaa cagttcattt tcctggcttg aaacattacg tcctgacgtg
     5821 ctgaaaattg ataagtcatt taccgcagct ataggttctg acgcggttaa ctcgacggtg
     5881 accgatatca tcatcgcact ggggcaaaga ctgaatattg aactggtggc ggagggtgtg
     5941 gaaacgcaag aacaggcgaa atatttgcgc cgtcatggcg tgcatatttt gcaagggtat
     6001 ttgtacgcac agccaatgcc gctacgtgat tttcccaaat ggctggcggg cagccaaccg
     6061 ccgcccgccc ggcataatgg acatatcaca cccattatgc cgttacgtta acgcttactc
     6121 atcttcatcg tgcaccggtt gctctttaac aatgcgaacc agatcaacac gataatcatt
     6181 ggcttcaatg atggtgatat gcagtggccc tacatcaatc acatcgccca cacggggaat
     6241 gtgaccattt gccgagatca cgaggcccgc gaccgtcgcg atatcatcgt catcggcaag
     6301 gtgctcaaca tcaagcgcct gctgcaaggc atgcaaatct gtaccgcctt ttaccagcca
     6361 gccgtcacca tcggtaatga tttccggcgt ttcgtctgcg tccgggaatt cacccgcaat
     6421 ggcttccagc acatccagcg gcgtgaccag accttgtacc acaccaaact cgttggtcac
     6481 gataacaaag ctcccgcgag cacgacgcag cacgcccagc aggttgatcg gatcgagggt
     6541 ttcagggacg ataatcgccg gagacgccga agcaatcgcc gccacatcaa cgccctcttc
     6601 cagcgccacc agcagttctt tagcacgtac gattccgatg atttcatcca gctcaccgcg
     6661 acataccggg aacaggctgt gcggtgagga gagcagctgc tcgcggattt catcgacccc
     6721 gagattagcg tcaacccagc taatttcacc gcgcggcgtc atgatcccgc gcagagaacg
     6781 cgacgccagc gtcagtacgc cgttaatcat gtaacgttct tcttcggcaa atgcaccttc
     6841 cgggatcggc atcggcatcg ggttatcggc atcgtgctga acattggcct gacgtttccc
     6901 gcccatcaaa cgcaggatgg catcggcagt acgcgctcgc agcggcaaag tcgactggtg
     6961 acgaataaag ttgcgacgcg caatttggtt aaacacttcg atgatgatcg agaagccaat
     7021 cgcggcatac aggtaacctt tcggaatgtg gaaaccgaaa ccttctgcca ccagactcag
     7081 accaatcatt aacaggaagc tcagacagag caccaccacc gtggggtgct ggttaacgaa
     7141 tcgcgtcagc ggtttcgatg ccagcaacat aaccgccatc gcaatcacta ccgccgccat
     7201 catcaccggc agatggttaa ccatccctac tgccgtaatt accgcatcca acgagaagac
     7261 ggcgtcaagg atgacgatct gtgtgacgac cacccagaaa ctggcgtagc ctttaccgtg
     7321 gccggaatca tgatcgcggt tttccagccg ttcatgcagt tcggttgttg ctttaaacag
     7381 caagaatatc cccccgaaca acataatcag gtcgcgtccg gagaaggaaa aatccataac
     7441 ggtaaatagc ggtttggtca gcgtgaccat ccatgaaatc agcgacagca gccccagacg
     7501 cataatcagc gccagtgata atcccagcaa acgcgcttta tcgcgttgtt ttggcggcag
     7561 tttgtcagca agaatggcga tgaagaccag gttatcgata cccagcacaa tttcgagaac
     7621 aacaagcgtg agtagccccg cccaaattga ggggtccatt aagaattcca taacaagctc
     7681 ctgcttaagg aatagctatt cgacgccaga aataatgcag gcgtaacgac aaaatgcaaa
     7741 cgaaaggtgc ggcatagagt gccagaaagg caggcgttaa aaggcctgat gctgaaatga
     7801 cgtcggtgac gatccatact gcgggctact gccctatact ccatggttgt taaacgggag
     7861 ttaaacatat cagagacgcc tctgatttgg caaaga
//
LOCUS       DAOTRK010000189         7878 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.190, whole genome shotgun sequence.
ACCESSION   DAOTRK010000189 DAOTRK010000000
VERSION     DAOTRK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.190"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(8..757)
                     /locus_tag="QIE41_004243"
     CDS             complement(8..757)
                     /locus_tag="QIE41_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDS3533046.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRKALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1058..1798
                     /locus_tag="QIE41_004244"
     CDS             1058..1798
                     /locus_tag="QIE41_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDS3533047.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1769..2536)
                     /locus_tag="QIE41_004245"
     CDS             complement(1769..2536)
                     /locus_tag="QIE41_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDS3533048.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2641..3219)
                     /gene="lpcA"
                     /locus_tag="QIE41_004246"
     CDS             complement(2641..3219)
                     /gene="lpcA"
                     /locus_tag="QIE41_004246"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDS3533049.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3459..5903
                     /gene="fadE"
                     /locus_tag="QIE41_004247"
     CDS             3459..5903
                     /gene="fadE"
                     /locus_tag="QIE41_004247"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDS3533050.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYKAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPDKVRKVEAA"
     gene            complement(5946..6392)
                     /gene="ivy"
                     /locus_tag="QIE41_004248"
     CDS             complement(5946..6392)
                     /gene="ivy"
                     /locus_tag="QIE41_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDS3533051.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6573..7343
                     /gene="yafV"
                     /locus_tag="QIE41_004249"
     CDS             6573..7343
                     /gene="yafV"
                     /locus_tag="QIE41_004249"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDS3533052.1"
                     /translation="MSGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            complement(7438..7749)
                     /locus_tag="QIE41_004250"
                     /pseudo
     CDS             complement(7438..7749)
                     /locus_tag="QIE41_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7768..>7878)
                     /locus_tag="QIE41_004251"
     CDS             complement(7768..>7878)
                     /locus_tag="QIE41_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDS3533053.1"
                     /translation="ILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1822 a         2018 c         2107 g         1931 t
ORIGIN      
        1 tacccattta caaaatcgtc tcttccgtca aaatccggcg cgcgcctaaa aaatggtcct
       61 gccagaaagg ttcagctaac cgacttaccc gtatggtttc cccggtgcgc ggcgactcaa
      121 taaactgtcc atcgcccaga tacacgccca tatggtcggc aatctcgcgg ctgtggatat
      181 gaaaaaacag caaatctccc cggcgcaggt cgttattcgc cacaatggtt gcccggcgat
      241 agtgatacat ctcattagcc gtgcgtggga gcttcgcctc gaggatcttg ttgtaggcat
      301 aaaaaaccaa cccgctacag tcgaagcctt gatcgggccg cgtgccgccc cagacgtaag
      361 gcttgccaag ctgctgctcc aggcggtgaa tcgccacttc ggtgatgttg tgtagatggt
      421 cgctgcttaa gaaatggttg ttctccgcca gcgcctgcca gcgtctgtca cttgccttga
      481 gcggcgcagg aaaccattct ggatgctgtt ttatctgttc gcggttgtgc tggcgcagcg
      541 cctttctgct ttcggcattg ccttccacaa tatagctggc ctgttttttc agatgggttt
      601 ggtactgttt taataaacgc gcacggtttt gcatccgctg gcgggcggcg tagctgaaag
      661 agatatgtga tgtggtggcg ctggcgagta aagggagcaa aacgcataac gaaaacacgc
      721 ctggatgcag aaatctgccg agcaagaacg aagacatgaa ggacataaac cggctgaatg
      781 aaggtgagaa ttttaaaaga ccagaggagt aaacgggatt attaattgct tatttgcata
      841 ttactattga ggtttcataa tcttaggaaa taaatgatac atgcgctttt cggactattg
      901 aaaactgccc ctgtgctaaa tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt
      961 tggtgataat gtgagccttc ccaatccaga ctattccgat ttgggatcca ttttgccgtc
     1021 ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg cgtaaaatcg cattaattct
     1081 tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac
     1141 cacgccagtg agcaaagagt ataagcagca gttgatggga tcccctgtct acatccagat
     1201 cttcaaggaa gaacgtacac tcgatctcta cgtcaaaatg ggcgagcaat atcaactgct
     1261 cgacagctat aaaatctgta aatattccgg cggcttaggg ccaaaacagc gtcagggcga
     1321 tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat cagttaaaac cagacagccg
     1381 ttactacaaa gcgattaata ttggattccc caatgcctat gaccgtgcgc atggttatga
     1441 agggaaatat ctgatgatcc acggcgattg tgtttccatc ggctgctacg cgatgaccaa
     1501 tcagggtatt gatgagatct tccagttcgt tactggtgcg ctggtttttg gtcagccgag
     1561 cgtgcaagtg agtatttacc cgttccgcat gaccgacgcc aatatgaagc gccataaata
     1621 ttccaacttt aaggacttct gggagcaact gaagccgggc tacgactact ttgagcaaac
     1681 ccgtaagcca ccgaccgttt ctgtggtcaa tggccgttac gtggtcagca agccgttaag
     1741 ccacgaagtt gtgcaaccac agctggcatc aaactacacg ctccccgagg caaaataagc
     1801 gccattcgcc tggcataatc ttttgccagg tttcattgcc cgtcagcggc tgtgtcgcaa
     1861 tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa gtcgatttcc acatcctgat
     1921 ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat ccagtgtaaa ttagtcgagc
     1981 aatacgccat tacatagcgc ccgtcagaaa gcagcatgtt gaaaacgccc ttctgccgca
     2041 gttcatccgc cagtgaggcg atatatttaa acactgccgc catgttgccc ggtgtgcgcg
     2101 ggtaacgctg cgttaattta tgcaggagcc agcagaaggc tttttcgctg tcggtttcac
     2161 cgaccgggcg gaagttgccg gtttccagtg atttgtatcc cgtcagttgt ccgttatggg
     2221 cataagtcca gttacgcccc cataactcgc gggtaaacgg gtgagtattt tccagcgcca
     2281 cctcgccccg attcgcctgg cgaatatgag ccaccaccga acaggatttt atcgggtagt
     2341 cctggacaag tttggcgatg ggggaattaa agctgggttg cggatcttta aatgtgcgac
     2401 agcctttacc ttcgtaaaag gtaatgcccc agccatcttt atgtggcccg gttccaccac
     2461 cacgctgtac aagtccggtg aaactaaagc agatatcggt agggacgttg gcgctcatcc
     2521 cgagcaattc gcacataggt aacccccaca acagcgggag cgcatggccc ccggcgaaga
     2581 ttgatttaag tattttcagg tcggatgcgg cgtaaacgca ttattcggcc tacgccagac
     2641 ttacttaacc atctcttttt caattaactg gatcaggata tggatcactt taatgtggat
     2701 ctcctgaatg cggtcggcat aaccaaagtg cggtacgcga atttcgatat ccgctgtgcc
     2761 agccatttta ccgccgtctt taccggtcag ggtgatcact ttcatcccct tctcacgcgc
     2821 cgccgcgatc gctttgatca cgtttgcaga gttaccggag gtggagatcc ccagtagtac
     2881 atcgccttcg cgacctaccg cttcaacgta acgggagaaa atatcgttga aaccaaaatc
     2941 gttaccgacg caggaaatat gactaacgtc agaaatggca atcgccggat agcccggacg
     3001 gttttcacgg tagcgccccg tcaactcttc ggcaaagtgc atagcgtcgc aatgggagcc
     3061 gccgttgcca caggaaagca ctttgccacc agctttaaaa ctgtctgcta acaggaccgc
     3121 cgcgcgctga atggcgtgaa tattggcgtc atcttttaaa aagttagcca gcgtttccgc
     3181 cgcttcgttc agttcgttac gaataagatc ctggtacatg aggatatcct tcagcataaa
     3241 tgtaatagac aaaatgcagt gtaccggata ccgccaaaag cgagaagtac gggcaggtgc
     3301 tatgaccagg actttttgac ctgaagtgcg gataaaaaca gcaacaatgt gagctttgtt
     3361 gtaattatat tgtaaacaca ttgctaaata tttttacatc cactacaacc atatcatcac
     3421 aagtggtcag acctcctaca agtaaggggc ttttcgttat gatgattttg agtattctcg
     3481 ctacggttgt cctgctcggc gcgttgttct atcaccgcgt gagcttattt atcagcagtc
     3541 tgattttgct cgcctggaca gccgccctcg gcgttgctgg tctgtggtcg gcgtgggtac
     3601 tggtgccgct ggccattatc ctcgtgccgt ttaactttgc acctatgcgt aagtcgatga
     3661 tttccgcgcc ggtatttcgc ggtttccgta aggtgatgcc gccgatgtcg cgcactgaga
     3721 aagaagcgat tgatgcgggc accacctggt gggagggcga cttgttccag ggcaagccgg
     3781 actggaaaaa gctgcataac tatccgcagc cgcgcctgac cgccgaagag caagcgtttc
     3841 tcgacggccc ggtagaagaa gcctgccgga tggcgaatga tttccagatc acccatgagc
     3901 tggcggatct gccgccggag ttgtgggcgt accttaaaga gcatcgtttc ttcgcgatga
     3961 ttatcaaaaa agagtacggc gggctggagt tctcggctta tgcccagtct cgcgtgctgc
     4021 aaaaactctc cggcgtgagc gggatcctgg cgattaccgt cggcgtgcca aactcattag
     4081 gcccgggcga gctgctgcaa cattacggca ctgacgagca gaaaaatcac tatctgccgc
     4141 gtctggcgcg tggtcaggag atcccttgct ttgcactgac aagccccgaa gcgggttccg
     4201 atgcgggcgc gattccggac accgggattg tctgcatggg cgaatggcag ggccagcagg
     4261 tgctgggcat gcgtctgacc tggaacaagc gttacattac gctggcaccg attgcgactg
     4321 tgcttggcct ggcgtttaaa ctctccgacc cggaaaaact gctgggtggc gcagaagatt
     4381 taggtattac ctgtgcgctg atcccgacca ccacgccggg cgtggaaatt ggtcgtcgcc
     4441 atttcccgct gaacgtaccg ttccagaacg gaccgacgcg cggtaaagat gtcttcgtac
     4501 cgatcgatta cattatcggc ggaccaaaaa tggccgggca aggctggcgg atgctggtgg
     4561 agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc ggtgtgaaat
     4621 cggtagcgct ggcaacgggc gcgtatgcgc acattcgccg tcagttcaaa atttctattg
     4681 gtaagatgga agggattgaa gagccgctgg cgcgtattgc cggtaatgcc tatgtgatgg
     4741 atgctgcggc atcgctgatt acctacggca ttatgctcgg tgaaaaacct gccgtgctgt
     4801 cggctatcgt taagtatcac tgtacccacc gcgggcagca gtcgattatt gatgcgatgg
     4861 atattaccgg cggtaaaggc attatgctcg ggcaaagcaa cttcctggcg cgtgcttacc
     4921 agggcgcacc gattgccatc accgttgaag gggctaacat tctgacccgc agcatgatga
     4981 tcttcggaca aggagcgatt cgttgccatc cgtacgtgct ggaagagatg gaagcggcga
     5041 agaacaatga cgttaacgcg ttcgataaac tgctgttcaa acatatcggt cacgtcggca
     5101 gtaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc agcacgccaa
     5161 ccggcgatgc cactaaacgc tactatcagc acctgaaccg cctgagcgcc aacctcgccc
     5221 tgctttctga tgtctcgatg gctgtgctgg gcggcagcct gaaacgtcgt gagcgtatct
     5281 cggcccgcct gggggatatt ttaagccagc tttacctcgc ctctgccgtt ctgaagcgtt
     5341 atgacgacga aggccgtaat gaagccgatc tgccgctggt gcactggggc gtacaggatg
     5401 cgctgtataa ggctgaacag gcgatggacg atttactgca aaacttcccg aaccgcgtgg
     5461 ttgccgggct gctgaatgta gtgatcttcc cgaccggacg tcattatctg gcaccgtctg
     5521 acaagctgga tcataaagtg gcgaagattt tacaagtgcc gaacgccacc cgttcccgca
     5581 ttggtcgcgg tcagtacctg acgccgagcg agcataatcc ggttggcttg ctggaagagg
     5641 cgctggtgga tgtgattgcc gccgacccaa ttcatcagcg gatctgtaaa gagctgggta
     5701 aaaacctgcc gtttacccgt ctggatgaac tggcgcataa cgcgctggcg aaggggctga
     5761 ttgataaaga tgaagccgct attctggtga aagctgaaga aagccgtctg cgcagtatta
     5821 acgttgatga ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg ccggataaag
     5881 tgcggaaagt tgaagccgcg taattgaata acggagccga aaggctccgt ttctttatcc
     5941 gctaattatt taaaattaaa gccatccgga tggttttcca ggctgccggt caacgccgcg
     6001 aacagcaccg ttttaccatc aatcgaaagc gcatcgttca cattcagcca ggtgagtttc
     6061 tcttgcgacg ttttctcatc aatagtcgag aacagccccg tcatctgatt agatttctcg
     6121 gaccacatca cagcgatacg ttgcgagcca cagtcatgcg gtttgcacgc gctcatcacc
     6181 tgatacgtct catctcccaa cgttacggtt tgtgcgggag tataagtacc gcctttcatc
     6241 acccatgcag gtagcttatg cccttgcacc atctgattaa acgcagcttt ggtggtttcg
     6301 ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca ttgcactggt ggcgatgacc
     6361 agagcggcga ctgtcgttat tgccttaaac atcattcctc ccgagcttat cctgcccatg
     6421 ttattaacct cctaaagcct ggcgataaca cgataatttg caaatcagat atttccaata
     6481 tcagcatttg ttatcaattt attctgcgct aaggcaactg cctgataagg tgttagagtt
     6541 ataaaaccct tcgcataaag gagttgtaca ccgtgtctgg tttgaagatt acgcttttac
     6601 agcaaccact ggtgtggatg gatggtcctg ccaacctgcg tcattttgat cgtcaactgg
     6661 aaggtattac cggacgcgat gtgatcgttc taccggagat gtttaccagc ggctttgcca
     6721 tggaagcggc agcttcgtcg ctagcacaag atgacgtagt gaactggatg acagccaaag
     6781 cgcagcagtg caatgcgctg attgcaggca gtgttgcatt acaaacggag tctggttcgg
     6841 ttaaccgctt tttgctggtt gagccgggcg gcacggtaca tttttatgat aagcgtcatc
     6901 tgttccgcat ggcagatgag catctacatt ataaagcggg caatgcgcga gtgattgtgg
     6961 aatggcgcgg ctggcgtatt ttgccgctgg tgtgctacga cttacgtttt cctgtgtggt
     7021 cgcgcaatct caacgattat gacctcgccc tatacgtcgc caactggcct gctccgcgct
     7081 ctctgcactg gcaggcattg ctgacggccc gcgcgattga gaatcaggcg tatgtggcgg
     7141 gatgcaatcg cgtcggcagc gatggcaacg gctgccatta tcgcggtgac agccgggtga
     7201 ttaatccaca gggcgagatt atcgctactg ccgacgcgca tcaggcaacg cgcattgatg
     7261 cggagctgtc gatggtggcg ttgcgggaat atagagaaaa gtttccggca tggcgggatg
     7321 cggatgagtt taggttgtgg tgagtgggtg ttgtgtttag gggcggggta agactacggg
     7381 tcagggcaag attacgaaag cccggagcct gtccatcatt ctgtgtaaat gcctttctca
     7441 gaagtgaccg tcaaggcggt caccgaactc gataataaag cgactcatcc ctatccgcca
     7501 gtcctgaagt ggcatcgtcc atttttgcga cgctgactgg aaagccagcc acaccacttt
     7561 tttcaccgac tcatctgtcg ggaacacttt ccgtttcttt atcgcatgtg tgatgatgct
     7621 attcagcgat tcgattgcgt tagtggtgta gataaccttg cggatcgcct ccgggtacac
     7681 gaagaatgtg gcaacattcg gccagtttgc ccgccagctg cgacttatct gcgggtaccg
     7741 tgcatcccat tccttcccag ggaaagatta cgaaagcccg ctccccgcaa ggactgacgc
     7801 cagatagttt ctgtccatgg ctgcttttcg catcttacgt cttaaccctg ccttgaatac
     7861 cttatcattc gtcaaaat
//
LOCUS       DAOTRK010000190         7736 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.191, whole genome shotgun sequence.
ACCESSION   DAOTRK010000190 DAOTRK010000000
VERSION     DAOTRK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.191"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            71..3763
                     /locus_tag="QIE41_004252"
     CDS             71..3763
                     /locus_tag="QIE41_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDS3533054.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSKGDRNPSSVRILIQLQRNGRWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAASRTLTLD
                     REVTLPETGTATVNLINGSGKPASVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVSTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEKRITNTAQVEKSARYLGTGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAF
                     VEASGQPSNDGEGYLEIFRGLIDETLLGQALKERIDASALRTEVTQLEEDIRQRMDTD
                     IAEVTRKIGEAENSLTQLVAKKNEDQTLAIAQVSQKVDRVSSEISQTVSQGQSENARQ
                     IAQVRQYVDKKGSEITSTTDKKLGDQAVTIQQIQRVQSDTRNELNAMYMLKVQKTKNG
                     IPYVAGIGAGIEDVDGQTLSSILLQADRIAMITPENGNTTPLFVAQGNQLFMNDVFLK
                     RLFAVSITSSGNPPTFSLTPDGRLAARNADISGAITANTGTLNNVTINENCVIRGKLS
                     ANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGARHE
                     RENNDIYSSCRLIVRKNGAEIYNRTALDNTLVYSGVIDMPAGRGHMTLEFSVSAWWVN
                     GWYPTASISDLLVVVMKKTTAGITIS"
     gene            3831..4430
                     /locus_tag="QIE41_004253"
     CDS             3831..4430
                     /locus_tag="QIE41_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HDS3533055.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDDRHSNTSLAW
                     GAGVQFNPTESVTIDIAYEGSGSGDWRTDAFIVGIGYRF"
     gene            4582..6645
                     /locus_tag="QIE41_004254"
     CDS             4582..6645
                     /locus_tag="QIE41_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDS3533056.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVTLLVEDFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVNEVARHAGASSQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAAASQT
                     ASANSATAAKKSETSAKNSETATKASEKNAKSSQTAAKTSETNAKDSEANAKVSETAA
                     ANSAKASAASQTAAKASEDAAREYANQTAEPYRYVLQPLPDVWIPFNDSLDMITGYSP
                     GYKKVKIGDNVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQR
                     TNFFQNSTDPSKWNKSTSLDVTETGTDSFGFNYGRFVVQDSIVGTSKAHTIIGLYSST
                     GGVDTSGDEKHVTISCRVKSEVDNIAVRILFEHYDGEVRTSIGAANLNLTTRIISKTG
                     QTSRVTARSVKDDATGWIFFEATLKADTTENTVGGFVQYSPDTGQMVTSGDYLDVTTP
                     QIEAGTGASSFIVTGTAPATRASDMVTVPIKNNLYNLPFTVLCEVHKNWYKTPNVAPR
                     VFDTGGHQTGAGIVMGFGSSGGYDGFPYCDIGGSDRRINENAGLEKMLIGMRVKSERS
                     TCVVSNGKLSSETKTKWEYIRSTATIRIGGQTTAGLRHLFGHVRNFRLWHKELTDAQL
                     GEVVE"
     gene            6642..6920
                     /locus_tag="QIE41_004255"
     CDS             6642..6920
                     /locus_tag="QIE41_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533057.1"
                     /translation="MRDFTLRFSDKADFRAFLRKLNWEEDEELQNAVLVDEIGFTFRE
                     TDVSDDGEPEYTRNEGYFVNIRLLDDGFEDSVFREWVVTPERPLREWF"
     gene            6930..7223
                     /locus_tag="QIE41_004256"
     CDS             6930..7223
                     /locus_tag="QIE41_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533058.1"
                     /translation="MDITSILHALCAVAVQVLAGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRINMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            7263..7361
                     /locus_tag="QIE41_004257"
     CDS             7263..7361
                     /locus_tag="QIE41_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDS3533059.1"
                     /translation="MLAAWKACRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(7416..>7736)
                     /locus_tag="QIE41_004258"
     CDS             complement(7416..>7736)
                     /locus_tag="QIE41_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDS3533060.1"
                     /translation="GQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT         1960 a         1795 c         2298 g         1683 t
ORIGIN      
        1 ttcgggcgaa ggatatccga cttctgtacg gaatggcaag tggcggttaa tttattcagg
       61 ggaaggctgt atgggaaaag gtggcggtaa ggcacacacg cctcgtgagg cgaaggataa
      121 tctcaaatcc acgcagatga tgagtgtgat tgatgcgatt ggtgagggac cgatagaagg
      181 tccggtgaag ggactgcaga gtattctggt gaacaaaacc ccgctgacgg acacggacgg
      241 taatcccgtg atacacggtg tgactgcggt ctggcgtgcc ggggagcagg agcagacacc
      301 accggaaggc tttgagtcct ccggagctga aaccggactg ggcgtggaag tgacgaaggc
      361 aaaaccggtg acgcgcacca ttacgtccgc gaacattgac cgcctgcggg ttaccttcgg
      421 ggtgcagtca ctggtgcaga ccacgtcaaa gggcgaccgt aatccttcct ctgtccggat
      481 tctgattcag ttacagcgta atggccgctg ggtgacggaa aaggatgtca ccattaacgg
      541 caagaccacc tcgcagttcc tggcctcggt gattctggat aatctgcctc cccggccctt
      601 taacatccgg atggtcaggg agacggcgga cagcaccacg gaccagctgc agaataagac
      661 gctgtggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggccat
      721 tgtggggctg caggtggatg cggagcagtt cggcggccag cagatgacgg tgaactacca
      781 tatccgcggt cgcatcatcc aggtgccgtc aaactatgac ccggaaaaac gcacgtacag
      841 tggtatctgg gacggcagcc tgaaaccggc atacagcaac aacccggcct ggtgtctgtg
      901 ggacatgctg actcacccgc gctacggcat gggaaaacgt ctgggggcgg cggatgtgga
      961 caagtgggcg ctgtatgcca tcgggcagta ctgcgaccag acggtcccgg atggtttcgg
     1021 ggggaccgag ccgcggatga cctttaatgc gtacctggca caacagcgta aggcgtggga
     1081 cgttctcagt gatttctgct ctgcgatgcg ctgtatgccg gtatggaacg gccagacgct
     1141 gacgttcgtt caggaccgtc cgtcggatgt ggtgtggccg tacaccaaca gcgatgtggt
     1201 ggtggatgat aacggcgtgg gtttccgcta cagcttcagt gccctgaagg accggcacac
     1261 ggcagtggag gtgaattaca ccgacccgca gaacggctgg cagacctcca cggaactggt
     1321 ggaagacccg gaagccatac tgcgctacgg acgcaacctg ctgaagatgg acgcgttcgg
     1381 ctgtaccagc cgcggtcagg cccaccgtgc cgggctgtgg gtgataaaga ccgaactgct
     1441 ggaaacgcag acggtggatt tcacgctcgg gtcacagggg ctgcgtcaca cacccggtga
     1501 catcattgaa atctgtgata acgactatgc cgggaccctg accggcggac gtgtcctgtc
     1561 catcgatgcc gccagccgta ccctgacgct ggaccgggag gtgacactgc cggagaccgg
     1621 cacggccact gttaatctga ttaacggcag cggtaagccg gcgagcgtgg ccatcaccgc
     1681 acaccccgcg cctgaccgga tacaggtcag caccctgccg gatggtgtgg agacatacgg
     1741 tgtgtgggga ctctccctgc cgtcactgcg tcgtcgcctg ttccgctgtg tctccattcg
     1801 ggaaaacacg gacggcacgt tcgccatcac ggcggtgcag cacgtaccgg aaaaagaagc
     1861 cattgtggat aacggggcca gctttgagcc gcagtcaggc agcctgaaca gcgttatccc
     1921 accggcagtg cagcacctga cggtggaggt gagtgcagct gacggtcagt atctggcaca
     1981 ggcgaaatgg gacacgccgc gggtggtgaa gggtgtgcgc ttcagtctgc gtctgaccag
     2041 cggaagcgga gaagacagcc gtctggtgag caccgccatc accgcagaca cggagcaccg
     2101 tttcagtggt ctgccgctgg gggaatacac cctgacggtg cgggccatta acagctacgg
     2161 ccagcagggc gaacctgcga ccaccacctt ccggattaac gcgcctgcaa aacccgccac
     2221 cattgagctg acgccggggt attttcagat aacggcggtc ccgcgtcttg cggtgtatga
     2281 cccgacggta cagtttgaat tctggttctc agaaaaacgc atcacgaaca cggcacaggt
     2341 ggaaaaatct gcccgttatc tggggaccgg cagtcagtgg actgtccagg ggagccggat
     2401 taagccgggg acggatttct ggttttacgt gcgaagcgtc aacctggtgg gaaaatctgc
     2461 ttttgtggaa gccagcgggc agcccagcaa tgatggtgaa gggtatctgg aaattttccg
     2521 ggggctgata gatgagacgc ttctgggcca ggcactgaaa gagcgcattg atgcttcagc
     2581 gctgcgtacg gaggtcacgc aactggaaga agacatccgt cagcggatgg acacggatat
     2641 cgcagaagtg acccggaaaa tcggggaggc ggaaaacagc ctcacgcagc tggttgcgaa
     2701 aaagaatgag gaccagacac tggccatcgc gcaggtgagc cagaaagtgg accgggtgag
     2761 cagtgaaatc tcacagactg tcagccaggg gcagtcagaa aatgcccgac agatagcaca
     2821 ggtccgccag tacgtggata aaaaagggag tgaaattacc tcgaccacgg ataaaaaact
     2881 gggtgaccag gccgtgacca tacagcaaat ccagcgggtt cagtcagaca cgcgcaatga
     2941 gctgaatgcc atgtatatgc tgaaggtgca gaaaacaaaa aacggtattc cctatgtggc
     3001 cgggattggt gcggggattg aggatgttga tggtcagacg ctgagcagta ttctgctgca
     3061 ggctgaccgt atcgcgatga ttaccccgga gaatggcaac accacgccgc tgtttgtggc
     3121 gcaggggaat cagctgttca tgaacgacgt gttcctgaag cgactgtttg cggtgagcat
     3181 cacgtcatcc ggcaatcctc ctacgttttc cctgacgccg gatggcaggc tggcagcccg
     3241 caatgcggat atcagtggag ccatcacggc gaataccggc acgctcaata atgtcaccat
     3301 taacgagaac tgtgtcatca gagggaaact gtctgcaaac cagattgaag gcgatctcgt
     3361 taaaacagtg ggtaaggctt tcccccgtga ctcccgtgca ccggagaggt ggccatcagg
     3421 gaccattacc gtcagggttt atgacgatca gccgtttgac cggcaaattg ttattccggc
     3481 ggtggcattc agcggtgcca gacatgagcg ggagaataac gatatttatt cgtcatgccg
     3541 cctgatagta cggaaaaacg gtgctgaaat ttataaccgt accgcgctgg ataatacgct
     3601 ggtttacagt ggtgttattg atatgcctgc tggtcgcggc cacatgacgc tggagttttc
     3661 tgtatcagca tggtgggtaa atggctggta tcccacagca agtatcagcg atttgctggt
     3721 tgttgtgatg aagaaaacca ctgcaggcat cacgattagc tgaattttat aaccccaata
     3781 cgggcgccag aaatggcgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaat
     3841 tatgtgctgt tattctgtcc gcagtagtct ggctggttgc cgctggtacg ccagcgagcg
     3901 cagcagagca tcagtccaca ctaagcgccg ggtatcttca gacccacact gatatgccag
     3961 gcagcgataa tctgaacggg attaacgtga aataccgtta tgagtttacg gacgcgctgg
     4021 ggctgattac gtccttcagt tatgccaatg ctgaggatga gcaaaaaacg cactacagcg
     4081 atacccgctg gcatgaagat tccgtgcgta accgctggtt cagcgtgatg gcggggccat
     4141 ctgtacgcgt gaatgaatgg ttcagtgctt atgcgatggc aggtgtggct tacagccgtg
     4201 tgtcgacgtt ctccggggat tatctccgcg taactgacaa caaggggaaa acgcacgatg
     4261 tgctgaccgg aagtgatgac gatcgccaca gcaacacgtc tctggcgtgg ggagctggcg
     4321 tgcagtttaa cccgaccgaa tccgtgacca ttgatattgc ttatgaaggt tccggtagtg
     4381 gcgactggcg aacggatgca tttattgttg gtatcggata ccgtttctga caacagacgc
     4441 cgatttatct tctgtaaata ttgttatgat acgcaggttc atccgcctta tggggtgaac
     4501 tgcgtttgag gaaacgtaaa gttacactgt cctgaagccc gtggcgtcac tgctgcgggc
     4561 tttttttatt ggtggaaaag tatgacagta aaaatttctg gcgtgcttaa agatggcaca
     4621 ggaaaaccag tacagaactg caccattgtg ctgaaggcca gacggaccag cagcacggtg
     4681 gtggtgaaca cggtggcctc tgaaaatccg gatgaagccg ggcgttacag catggatgtt
     4741 gagtatggcc agtacagcgt caccctgctg gttgaagatt ttccgccttc acatgccggg
     4801 accattaccg tctatgaagg ttccagacca ggtacgctga atgattttct cggcgccatg
     4861 acggaggatg atgttcgtcc ggaggcactg cgccgttttg agctgatggt gaatgaagtg
     4921 gcacgtcatg ccggagcgtc atcacagagt gcagcggcgg caaagaaatc cgaaacggca
     4981 gcagcctcat cgaagaatgc ggcgaaaacc tcagaaacga atgcagctaa cagcgcacag
     5041 gcggcagcgg cctcgcagac tgcatcggca aactccgcga cagcagccaa aaaatcagaa
     5101 accagcgcga aaaatagcga gacagccaca aaggccagcg aaaaaaacgc aaaatccagc
     5161 cagacggcag cgaaaaccag tgagacgaat gccaaagaca gtgaagccaa cgcaaaggtg
     5221 agcgaaacag cggcggcgaa ctcggcgaaa gcatcggcag caagccagac ggcagcaaaa
     5281 gcaagtgaag atgctgccag agaatacgca aaccagacag cagagccgta cagatatgtt
     5341 ttacagccgc tgccggatgt gtggataccc tttaatgatt cgctggatat gattacgggc
     5401 tattctccgg gttataaaaa agtgaagatt ggtgataatg tggttcaggt tgccagtgat
     5461 aaacaggtta atttcagtcg cgcatcaacg gcaacatata tcaacaaatc tggcgaactg
     5521 aaaacggcgg aaattaatga gccgcgattt gagtgtgatg gcctgcttat tgagggacaa
     5581 agaacgaact tcttccagaa cagtacagac ccttcgaagt ggaataagtc aacttcactg
     5641 gacgttacag aaacaggcac agatagtttc gggtttaatt atggtcggtt tgtcgtacag
     5701 gattcgattg ttggtacaag taaagcgcat accattatcg gactgtattc gagtaccgga
     5761 ggggttgata cttcagggga cgaaaagcat gtaactatat cctgtcgggt aaaaagtgaa
     5821 gttgataata tcgccgttcg tattttattt gaacattatg atggggaggt aaggacatca
     5881 ataggagcag caaacctgaa ccttaccacc cgcataatta gcaagacagg tcagacaagc
     5941 cgtgttacag caaggtctgt taaggatgat gcaactggct ggatattttt tgaggctaca
     6001 ttaaaagcag atacaacaga aaatacggtt ggtggttttg tccagtattc tccggataca
     6061 gggcagatgg ttacatcagg ggattatctc gatgtaacca ctccacagat tgaggctggt
     6121 acaggcgcat catcttttat tgttacgggg acggcaccgg caacgcgggc aagcgatatg
     6181 gtgacagtcc caatcaagaa taacctttat aatcttcctt ttacggttct ttgtgaggta
     6241 cataagaact ggtataaaac gccaaatgta gcgccgcgtg tttttgatac cggcggtcat
     6301 caaaccggag cggggatcgt aatggggttt ggttcatcag gtgggtacga cggttttccg
     6361 tattgcgata taggtggttc agaccgacga ataaatgaaa atgccgggct ggaaaaaatg
     6421 cttattggta tgcgggtaaa gtccgaacgg tccacatgtg tagtcagtaa cggtaagtta
     6481 agcagcgaaa ctaaaaccaa atgggaatat atccggagta cagcaaccat tcgcattggt
     6541 ggacaaacta cagcaggatt acgccattta tttgggcatg tgaggaattt tcgtctctgg
     6601 cataaagagc taacagatgc gcagcttggg gaggttgtgg agtgagagat ttcacgttgc
     6661 gtttcagtga taaagcagat ttcagggcat ttctcaggaa acttaactgg gaagaggacg
     6721 aagagctgca gaatgccgtt ctggttgatg agattggttt tacgttcagg gagacagatg
     6781 tttctgatga cggagaacca gaatacacgc gaaacgaagg gtactttgtt aatatccgtc
     6841 ttcttgacga tggatttgag gattccgtgt tccgtgagtg ggtggttaca ccagagcgcc
     6901 cgctcaggga gtggttttaa ggatagcaga tggatatcac gtcgatactt catgcgcttt
     6961 gtgccgtggc ggtgcaggta ctggctggtc tttttaccgg aaactgggct tacggggcga
     7021 tagccggttg tacgttcttc attgcgcgtg aacacaccca ggcagaatat cgctggattg
     7081 aaatgttcgg gcatggcaag cggattaaca tgccgtggtg gggcggtttt gatccacgtg
     7141 catgggatgt ggcaagcctg atggattttg ctgtgccggt ggtggcgtgt ctgctggtct
     7201 ggctgttggt taatcgtggg tgaaaaaggt gagcagtata tgcaacgaag gaggaaacat
     7261 cattgctggc ggcatggaag gcatgcaggg tgttgctgaa ccgtgttgat acatcaactg
     7321 cacctgatat tgagtggcct acgaaccctg tcagggagta atcattggga ttatgccgca
     7381 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     7441 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     7501 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     7561 atcagttttt gaccgaagct attgtgcaca acgccgtcac caagaattgt ggctccgtat
     7621 agagttccat cgtcagttaa ggcctgcgcc gcattgcgta ttacacagct ttttgtagat
     7681 atatttccag gcaggcagtg aagaaggtaa aacatggaaa tggaatcaaa ttgacc
//
LOCUS       DAOTRK010000191         7638 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.192, whole genome shotgun sequence.
ACCESSION   DAOTRK010000191 DAOTRK010000000
VERSION     DAOTRK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.192"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(22..1440)
                     /gene="araE"
                     /locus_tag="QIE41_004259"
     CDS             complement(22..1440)
                     /gene="araE"
                     /locus_tag="QIE41_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDS3533061.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(1755..2516)
                     /gene="kduD"
                     /locus_tag="QIE41_004260"
     CDS             complement(1755..2516)
                     /gene="kduD"
                     /locus_tag="QIE41_004260"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDS3533062.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(2546..3382)
                     /gene="kduI"
                     /locus_tag="QIE41_004261"
     CDS             complement(2546..3382)
                     /gene="kduI"
                     /locus_tag="QIE41_004261"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDS3533063.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(3669..4850)
                     /locus_tag="QIE41_004262"
     CDS             complement(3669..4850)
                     /locus_tag="QIE41_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDS3533064.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVS
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            5105..6334
                     /locus_tag="QIE41_004263"
     CDS             5105..6334
                     /locus_tag="QIE41_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDS3533065.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            6794..7426
                     /locus_tag="QIE41_004264"
     CDS             6794..7426
                     /locus_tag="QIE41_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3533066.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         2142 a         1868 c         1626 g         2002 t
ORIGIN      
        1 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
       61 tttgcgttcg atatgttcca gggtgacatt tttggtttcc ggaatgagcc agaaagtgat
      121 gcccacaaac gcaatgttca gcgcagtgta gagccagaat gtaccggcag cgccaatgct
      181 atcaagcagt gtcaggaagg tcgcgccgat aatcatattc gacacccagt ttgtcgtcgt
      241 tgagcaggtg atgccaaaat cgcggcattt cagcggctga atttcagagc acaagatcca
      301 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
      361 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc agtaacccag
      421 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggct tacgccctgc
      481 tttatctacc gtaaagaccg caatgaaggt ggcgaacata aaggtcagcc ccacgaccag
      541 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
      601 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
      661 aaatacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
      721 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
      781 catacgcaat acttcttccg cctcaatatg acgccctttt tccgccagcc agcgcgggct
      841 atttggcagg aagacaacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
      901 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
      961 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcactggc
     1021 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
     1081 cacacgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tcgagccgag
     1141 cacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
     1201 accattaaac agcgcaccaa ttgccgcgcc gagcatcatg ctactgacca cccattcctg
     1261 caaacggctg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
     1321 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
     1381 acgccgcgta tcccgcagag gacgtggcgt taaagcagat tccgtattga tagtaaccat
     1441 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaat tcacacaggt
     1501 gtcaggtcgg aaatagcata aatatggatt aaattgctac gacatgtcgt tatgtgatgg
     1561 atattccaat tttcaaatta agttgaataa ttgagattat tattaaccac ctaattttaa
     1621 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
     1681 gatatggatt atgatgatct acaggtataa aaaaccctgc catgcggcag ggtcataaaa
     1741 gtaagagaaa tgaattaacg agccagccaa ccgccatcca cggcaatggt ataaccattc
     1801 acataatctg aagcgctgga ggcaaggaac actaccggcc ccatcaggtc actcggcagt
     1861 ccccaacggc cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
     1921 agttgttgag tattgttggt tgccatgtaa cccggagcta tcgcattaac attaatgttg
     1981 tgtttagccc attcattcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
     2041 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgcgat attgataatc
     2101 ttgccgccat tgccttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
     2161 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
     2221 tcgcgccgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
     2281 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
     2341 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
     2401 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctgacc cagtccagta
     2461 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
     2521 ataccttatt cgtttatgcc cacaactagc gcaaatcttt aacggccaca tggtccatat
     2581 catcaaagac ctggttttca ccgaccatgc cccagataaa ggtataagct ttggttccga
     2641 caccggaatg gatcgaccag ctcggggaga tcaccgcctg ctcgttatgc atcacaatat
     2701 gacgcgtttc ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga
     2761 aatagaaata aacttccatc cggcgctcgt gggtgtgaca cggcatggtg ttccacaagt
     2821 tacccggagc cagctccgtc agccccatac tcaattggca ggtttccagt acatccggga
     2881 caaaatattt gttaatcgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag
     2941 atacttcgtc cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt
     3001 aataaaactt cgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt
     3061 taccaacata cagggcgtcg cggtgaccga tttcatagca ttgaccatcg acagtaatcg
     3121 taccggcacc gccaatattg ataacaccta actcgcgacg ttccaggaaa tagcttacgc
     3181 cgagttgttt accaacttcc ccgccaacgg aaaccgtttt agttaccggc ataatgccgc
     3241 caacaattat acggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct
     3301 tttcaaccaa aaattcattg cgcagcccct gggtatccag cgtttttgca tgcgcactgt
     3361 ggatgctctg tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa
     3421 acgatagtga aacgctgttt cgttttctct ataaggggat gatatgccca cgggataggg
     3481 ttttcaatta aaaataaaac aatgtttcat ttttaagtta aggactgaaa aaagtgcagt
     3541 gtcgatcacg aatatgtcat gagaatggaa aaatcaggga gccctgaaaa tgtatgtcga
     3601 ctcgccagcc accattattc aacagattaa tgacggctga tatttatcgg taaggaggaa
     3661 atgcagagct attcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa
     3721 agcgtagcca gtcctttgcg ggcattacgt ttcaccattt catgaaccag agaaacgagg
     3781 attcggcaac cggaagcgcc tattgggtga ccgagtgcga tcgcgccacc attgacattg
     3841 acccgacgct catcccattc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct
     3901 tcattagcct cgataagatc gacatcagcc aactgccagc caacacgctc caggcaacgg
     3961 cgggtcgcat acaccggcgc aattcccatc aatgccggat ctacaccaac actggcaaat
     4021 gcgcggatgc gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc
     4081 attactgcag ctgcgccatc gtttatggat gatgcattac ccgctgtcac agagccgaga
     4141 ctatcaaatg aaggatttaa acgggctaag ccttctgcac tggcgtcagt gcgtggctgt
     4201 tcatcggtat caacaaccaa cgtctgaccg ttactttggg tcattaccgg gacgatctca
     4261 tctttaaatc gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa
     4321 gcatcctgca actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca
     4381 atatgataat cattgaaggc atcccacaac ccatcgtgca caagactgtc aaccaactgg
     4441 ctattgccaa gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcgactcatg
     4501 ttttcctgac caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc
     4561 agatgcagtg ctttaagccc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta
     4621 ttaggaagac cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc
     4681 aacacctgac caagaatcac ttcatccacc gcatatgcag aaacaccggt acgttctatt
     4741 aatgctttca cgaccaggct gccaagttcc acggcggaat gacccgctaa cgcaccacga
     4801 aagcagccga taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg
     4861 caccaattaa cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa
     4921 aaagtgattt ttatcacaaa ggaaatacgc ctgaccagca gtcagagaca taactggcac
     4981 gtaaggtttg caaccactaa cccaccaata gagaggtaga aagggcgtta atctcccata
     5041 cttaacctgg tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca
     5101 gataatgtct aatatttggt caaaagaaga aactctgtgg agtttcgcgc tctacggcac
     5161 agccgttggt gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt
     5221 cctgtttatt actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg
     5281 ccagttcatc ctctcatcaa aaacatcagc aggtgaaggg ataacgggcg cggtaacaca
     5341 ctactatggc aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt
     5401 cgtcgtgttg atatatgccg tggcaattac caactcactt acggaacagc tggcaaagca
     5461 tatggttatt gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taatcctgaa
     5521 tctcattttt ctgatgggac gccatgccac tattcgggta atgggatttt tggtattccc
     5581 attgattgcc tatttcttat ttctttctat ttacctggtc ggtagttggc aacctgatct
     5641 attaactacc caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc
     5701 cgtgatggtt ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg
     5761 tgaaaaatat ggcgaacatg ctatggataa atgcaaaaaa attatgaaag tcgcttatct
     5821 catcatctgc ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc
     5881 gtatattgaa gcggctaaag aagaaggggt taccatttta tcggcgcttt ctatgctgcc
     5941 gaacgcccca gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa
     6001 atcattcctg ggtacgtact ttggcgttat tgaaggtgcc acagaggtcg tcaaaacaac
     6061 actacagcag gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt
     6121 ggtatcgctg attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta
     6181 cgcgatcagc ggcccgctca ttgccatgat acttttcatc atgcctacgc tgtcaacgta
     6241 tctcatcccg gcgcttaaac cctggcgttc catcggaaat ctgattacgc tgatcgtggg
     6301 tatcctgtgc gtatcggtaa tgttctttag ctaatatcca ttgtgaatgg ttcaaaattg
     6361 ctacaatttt gaaccattcc agttttcata atgttcattt gttataactc cgctctatca
     6421 cttctctcgt catatcctag tattatcagt tagttaaaca cacaaagaga tctgaacatc
     6481 ttaaataaga ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa
     6541 aaacaatttg cgatatagat attctcatac catgagatag ttatctgaac caccatcaac
     6601 caggtaagat taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa
     6661 tgctttactc agaattatag caaattcaga ttaatcgcaa tttttcccag caaatttaca
     6721 aaatcatggt gtattgacat catcaataac caatgaaatt atgcaattat atacggatag
     6781 ggaggttctt aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc
     6841 aatgcattgc caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg
     6901 tttttgtatg tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatttt
     6961 aacaaaaaac cacaatgcga gattaagcgg atatattttt gtcgattttt cagtaagttt
     7021 tttacgtcta ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat
     7081 tgttttagtc agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaactc
     7141 tgcaatatct gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag
     7201 acaactattt attggtcgtt atttatcatt taccagaggg aacacattaa ctcagatgga
     7261 attcaccata atgggatata tggtttctgg ttataatcca tatcagattg ctgaagttct
     7321 ggatatggat atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg
     7381 taaaataaac gagttattta ttcgttcaca ttcggtccaa cattgatact aaataatctg
     7441 ccagaatcat actctgctta tacgggcatc tattgaaatg cccgtatttt acaaataagc
     7501 agtaacccca agagtaaaca acatggtatt taaaaccatc acacgaaaaa ttcagaagca
     7561 ttacgaaata tgaatttaca aacaatagtg gcataaatgt taatcatgtt aatttacgta
     7621 aagttttacg ttgcgaca
//
LOCUS       DAOTRK010000192         7356 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.193, whole genome shotgun sequence.
ACCESSION   DAOTRK010000192 DAOTRK010000000
VERSION     DAOTRK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.193"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            151..339
                     /gene="dicB"
                     /locus_tag="QIE41_004265"
                     /gene_synonym="ftsT"
     CDS             151..339
                     /gene="dicB"
                     /locus_tag="QIE41_004265"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDS3533067.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            336..527
                     /gene="ydfD"
                     /locus_tag="QIE41_004266"
     CDS             336..527
                     /gene="ydfD"
                     /locus_tag="QIE41_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDS3533068.1"
                     /translation="MNSAFVLVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKISGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            620..3091
                     /locus_tag="QIE41_004267"
     CDS             620..3091
                     /locus_tag="QIE41_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDS3533069.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGLQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPNQADF"
     gene            3156..3404
                     /locus_tag="QIE41_004268"
     CDS             3156..3404
                     /locus_tag="QIE41_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDS3533070.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            3382..4512
                     /locus_tag="QIE41_004269"
     CDS             3382..4512
                     /locus_tag="QIE41_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HDS3533071.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(4558..5196)
                     /gene="ompW"
                     /locus_tag="QIE41_004270"
     CDS             complement(4558..5196)
                     /gene="ompW"
                     /locus_tag="QIE41_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDS3533072.1"
                     /translation="MKKLTVAALAVTTLLSGSAYAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            5436..5525
                     /locus_tag="QIE41_004271"
                     /pseudo
     CDS             5436..5525
                     /locus_tag="QIE41_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5553..6296
                     /locus_tag="QIE41_004272"
     CDS             5553..6296
                     /locus_tag="QIE41_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDS3533073.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSTLRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            6326..6865
                     /locus_tag="QIE41_004273"
     CDS             6326..6865
                     /locus_tag="QIE41_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDS3533074.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATALVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            6970..>7356
                     /gene="yciA"
                     /locus_tag="QIE41_004274"
     CDS             6970..>7356
                     /gene="yciA"
                     /locus_tag="QIE41_004274"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDS3533075.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRAL"
BASE COUNT         2045 a         1701 c         1783 g         1827 t
ORIGIN      
        1 ggtgaatgcg gctgagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat
       61 tctctgtatc cggcgttaat tgttaactgg ttaacgtcac ctggaggcac caggcactgc
      121 atcacaaaat tcattgttga ggacgcgata atgaaaacgt tattaccaaa cgttaatacg
      181 tctgaaggtt gttttgaaat tggtgtcact atcagtaacc cagtatttac tgaagatgcc
      241 attaacaaga gaaaacaaga acgggagcta ttaaataaaa tatgcattgt ttcaatgctg
      301 gctcgtttac gtctgatgcc aaaaggatgt gcacaatgaa ttcagcattt gtgcttgttc
      361 tgacagtttt tcttgtttcc ggagagccag ttgatattgc agtcagtgtt cacaggacaa
      421 tgcaggagtg tatgactgca gcaaccgaac agaaaatttc cggtaactgt tacccggtcg
      481 ataaagttat tcaccaggat aatatcgaaa tcccggcagg tctttaaaac agttccgtaa
      541 taaatatccg gtttcattct tatatgccag caatggcagg gatttgttca tccttaaatc
      601 tgtcatgagg ttaaaacaaa tgagtaaagt ctttatttgc gccgctattc ctgacgaact
      661 ggcaacaagg gaagaaggcg ctgtggctgt agccacagcc attgaagctg gcgacgaacg
      721 ccgtgctcga gcaaaatttc actggcaatt cctggaacat tatccggctg ctcaggactg
      781 cgcttataaa tttattgtct gcgaggataa acctggcata ccccgccctg ccctcgattc
      841 atgggatgct gaatatatgc aggaaaaccg ctgggatgag gagtctgctt cttttgtccc
      901 ggttgagact gaatccgatc cgatgaacgt cacttttgac aagctggccc ctgaagtaca
      961 gaacgctgtc atggttaagt tcgacacatg tgaaaacatc accgttgata tggttattag
     1021 cgcacaggaa ttgttgcagg aagacatggc aacattcgac ggacatatcg ttgaagcgtt
     1081 gatgaaaatg ccagaagtta acgccatgta tccggagctt aagttgcacg ccattgggtg
     1141 ggttaagcat aaatgtattc ctggtgctaa atggcccgaa attcaggcag agatgcgcat
     1201 ctggaaaaaa cgtcgcgaag gtgaacgcaa ggaaaccgga aaatacacgt ctgttgttga
     1261 tctcgcccgc gccagagcca atcaacagta cactgaaaat tcaacaggaa aaatcagccc
     1321 ggtcattgct gccattcatc gcgaatacaa gcagacatgg aaaacactgg atgacgaact
     1381 ggcctacgct ctctggcctg gtgatgtgga tgccggaaac attgacggca gcatccatcg
     1441 ctgggcaaaa aaagaagtta tcgacaacga ccgcgaagac tggaagcgta tctcggcatc
     1501 aatgcgcaaa cagcctgatg cccttcgcta cgaccgccaa actatttttg gccttgtccg
     1561 tgagcgtccg atcgacattc acaaagatcc cgtagcactg aacaaatata tctgcgaata
     1621 cctgacgaca aagggcgtgt ttgagaatga agaaacagac ctgggcactg ttgatgttct
     1681 ccagtcatca gaaacacaaa ctgatgcagt ggaaactgag gtatctgata tcccaaaaaa
     1741 tgaaaccgcg ccggaagctg aaccatctgt agagcgtgag gggccgttct atttcctctt
     1801 cgcagataag gacggagaaa aatacggtcg cgcaaacaaa ctctctggtc tggataaggc
     1861 actggctgct ggcgccactg aaatcacaaa agaagaatat tttgcccgaa aaaatggcac
     1921 atacacgggc ttaccgcaaa atgtagatac cgctgaagat tcagaacaac cagagccgat
     1981 aaaagttacc gctgacgaag taaacaaaat tatgcaggca gccaatatca gccagcctga
     2041 cgccgataag ttgcttgctg catcacgtgg tgaatttgtt gaagggatta gtgacccgaa
     2101 tgatccgaaa tgggttaagg ggctccagac ccgcgattct gtgaaccaga accagcatga
     2161 atcggaacgg aactaccaaa aagcggaaca aaacagtcca aatgcgttac aaaacgagcc
     2221 agaaacgaaa cagcctgaac cagtagcgca acaggaagtg gaaaaagtct gcaccgcctg
     2281 cggtcagacc ggcggcggca actgccctga ttgtggcgcg gtgatgggcg acgcaacata
     2341 ccaagaaaca ttcgatgaag agtatcaggt tgaagttcag gaagatgatc cggaggaaat
     2401 ggaaggcgct gaacatccac acaaggagaa cactggcggc aatcagcatc acaatagcga
     2461 taatgaaact ggcgagacgg cagatcaccc aattaaggtg aacggtcatc acgaaatcac
     2521 atccaccagc aggacgtgtg accatctaat gatcgacctt gaaaccatgg gaaaaaatcc
     2581 tgatgccccg atcatctcaa taggtgcaat atttttcgat ccgcaaaccg gagatatggg
     2641 accggaattt agtaagacta tcgatctgga aactgctggc ggagtcattg atcgggacac
     2701 cattaaatgg tggcttaagc aatcacgcga agcgcaatct gccattatga ccgatgaaat
     2761 cccgttagat gatgcactgt tacaattgcg agaatttatc gacgaaaact ccggtgaatt
     2821 ttttgttcag gtttggggaa atggagccaa cttcgacaac acgattttgc gccgttcata
     2881 cgaacggcag gggatcccct gcccgtggcg ttactacaac gatcgcgatg tacgcacaat
     2941 cgttgagctg gggaaagcca tagacttcga tgccagaacg gctattccat tcgaaggtga
     3001 gcgccataat gcacttgatg acgcccgtta ccaggcaaaa tacgtttcag ctatctggca
     3061 aaaactgatc ccaaatcagg ctgattttta atgttcaacc ccggtcgttg cccaccagct
     3121 atagtggcgg cgaccatgat tagcgaacga cgctcatggc aagacttatt ctgctcactg
     3181 agtgggcaaa ggaggaattc agtgaaccgg tccctactcc gagtacgtta agtaaatacg
     3241 ctaaagccgg aatgatattt cctctcccca aaaaagttgg aagacgctgg cgagtggatc
     3301 cgcaagctcg ctttgtcgga atggtaaaca agccggaggt gatcgccaca gatcaccctg
     3361 ctttgaagag gatactggaa gatggcgcgc ccgcgaaaat ataaaaccga tgttccggga
     3421 ttatctccgt attttgacaa aagaaataac aaagtttact ggcgttacag gcatcccata
     3481 acaggcaaaa atcacggtct cggcagtatt gaccagaaac tggcagaaac tattgcagca
     3541 gaagcgaaca gccgtcttgc ccggcagcaa atggaacaaa tgctcagtct gcaggagaaa
     3601 attattagtg acaccggcgg ttcatcaacc gttaccattt ttctgaataa ttacagaaaa
     3661 attcaacagg aaagatatga aaacggcgag atcaaactca acacgctgaa acagaaagcg
     3721 gcccctctca gggtatttga tgaacgtttt ggcaccagac cgttagatgc cataaccgta
     3781 aaagatgtgg tatcagtact ggaagagtac aaggccagag gacataacag aatgggacaa
     3841 attttcagga aagtactgat cgatgttttc cgggaagctc agcaaacggg cgatgtcccg
     3901 ccaggcttta accctgcaga atcggcaaaa aaaccgcagg tgcggatatc aagacagcga
     3961 ctgacttttg atgagtggat gatgatttat aacgcagcgg aaaaggatgg ttacttttta
     4021 cagcgcggta tgctgctggc actgatgaca ggccagcgcc tttcagatat ttgcaaaatg
     4081 caattttcgg atatccggga tggttatctt catgtcgaac agcaaaaaac aggaacccgg
     4141 attgccatcc ctctggctct gcgttgcgat aaattaaatc tcaccctaga tgatgtggtg
     4201 tcatcctgcc gcgattgcgt tcttagtccg tggctattgc accaccatca cgcgaaaggg
     4261 acagctaagc gcggcgggat ggttaagcca gcaacattaa ccgttgcatt taaaaaagcc
     4321 cgggattctg tggattacaa ctggcgtgct aatggcaccc caccctcttt ccatgagcag
     4381 agatctttat cagagcgatt gttcagagag cagggggttg ataccaaaat tttgctgggc
     4441 cattcgaatc aaaaaatgac cgatatttac aacgacgcac gcggtaagga atggaaaaaa
     4501 ctggtcattt gatgaccagt tttgcagagg ggttttgcag aggttttgca gagaaaatta
     4561 aaaacgatat cctgcggaga acataaacac ccacggatcc agtcgtaccg agtcttttac
     4621 ggtagtaaca ccagatttgt acttagccgt ggtatcgata tccatgtacc acactgacat
     4681 gttaaccaac cagtcacggt taatcagata atcaaccccc acctgcccgg cagctcccca
     4741 ggagtcttta agactgagat cggaaagccc cgcctctttg ccatgatcgt taaatccatt
     4801 atcaaagaag gtggtgtagt taatacctgc accaacgtaa ggacggaatt tgctgctggc
     4861 atcaccaaaa taccactgcg ccatcagtgt tggcggcaga tgatgaacgg ttgcaatatc
     4921 gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc gctgccagca attccacacc
     4981 aatgttatcg gtcgccatat aagtaaacgt caggcccagt tgcgtgttat tggtcacgct
     5041 gaatccaccc agacttccta acgtaccacc agcaccttct gttggacgta cggttgcaga
     5101 ccctgcacgc ataaaaaatt cgcctgcttc atgcgcatag gcactgccag agagaagagt
     5161 tgttactgcc aaagccgcca ctgttaactt tttcatatcc gctccgtcgt tatggttata
     5221 aaaacgtgac gaatatacct acattggagt aacaaatgat cctacataga tcacattaca
     5281 tatgataatt caccatttat tgatctggat taatttagaa gttgcattga gaatcctggt
     5341 taatgtgatt taaatcaatt tttaaatatt ttcgctacgc aaatattaac tcgttcgcca
     5401 cttgccgcgg tttgcccacg cctgaaggcc agcaattacc tccgctttac aaagatatgc
     5461 ttttccatac aagacctgat gctgccaccc gaggcttaac cagggtacaa ttgcccgcta
     5521 attaacacct gcataaaact caaggagagt gcatgtctat cacggcgcag tccgtatacc
     5581 gtgacaccgg aaatttcttc cgtaatcaat ttatgaccat tctgttggta tcgttgctat
     5641 gtgcgtttat cacagtggtg ttagggcatg ttttctcacc cagtgatgca cagcttgcgc
     5701 agctcaatga cggcgtgccc gttagcggca gtagtgggtt gttcgacctg gttcagaata
     5761 tgtcaccgga acagcaacaa attttgctgc aggcttcagc ggcgtccact ttttcaggat
     5821 taatcggtaa cgccattctc gccggaggcg taatattaat tatccagctg gtgtctgcgg
     5881 gtcagagagt cagtacgctg cgagctattg gtgccagtgc gccgatattg ccaaagttat
     5941 ttattctgat ttttctgact acccttttag tacagattgg catcatgctg gtggtcgttc
     6001 cgggaattat catggccatt ttactggccc tggcaccggt gatgttggta caggacaaaa
     6061 tgggcgtttt tgcctcgatg cgtagcagta tgcggctgac ttgggcgaat atgcgtctgg
     6121 tggcacccgc agtactgagc tggttgctgg caaaaacact gttgctgctt tttgcctctt
     6181 cttttgccgc attaaccccg gaaattggtg ccgtacttgc gaacaccttg agcaacctga
     6241 tttcagccat actgctcatc tatctgttcc gcctgtatat gttgattcgc caataacctt
     6301 aagcatttga ttacggaatc gtaaaatgaa gcagtttctt gattttttac cgctggttgt
     6361 ctttttcgcg ttttacaaga tttatgacat ctatgcggct actgcggcgc tgatcgtcgc
     6421 cacggcgctt gtgcttatat atagctgggt tcgctttcgt aaggttgaga agatggccct
     6481 gatcactttt gttctggtgg tcgtcttcgg tggcttgacc ctgttcttcc acaatgatga
     6541 gtttattaaa tggaaggtta ctgtcattta tgccctgttt gcgggtgccc tgttagtcag
     6601 ccaatgggtg atgaaaaagc cgctaattca gcggatgctg ggtaaagaac tcacgctgcc
     6661 gcaaccggta tggtcgaagc tgaatctggc ctgggctgtt ttctttatcc tttgcggtct
     6721 ggcaaacatc tacatcgcat tctggctgcc gcaaaatatt tgggtcaact ttaaagtctt
     6781 tggcctgacc gcccttacct taatcttcac attgttaagc ggtatctata tctaccgcca
     6841 catgccgcag gaagataaat cctaaccaga ctgccagtcc ttcggggctg gcgctgtatc
     6901 tcctctgttc tcctcaggaa aatcatagta gcatcgcgcc tgtgattttc cttttaagtc
     6961 ggttttacca tgtctacaac acataacgtc cctcagggcg atcttgtttt acgtacttta
     7021 gccatgcccg ccgataccaa tgccaatggt gacatctttg gtggttggtt aatgtcacaa
     7081 atggatattg gcggcgctat tctggcgaaa gaaattgccc acggtcgcgt agtgaccgtg
     7141 cgggttgaag gaatgacttt cttacgaccg gttgcggtcg gcgatgtggt gtgctgctat
     7201 gcacgctgtg tccagaaagg gacgacatcg gttagcatta atattgaagt gtgggtgaaa
     7261 aaagtcgcgt ctgaacccat tgggcaacgc tataaagcga cagaagcatt atttaagtat
     7321 gtcgcggttg atcctgaagg aaaacctcgc gcctta
//
LOCUS       DAOTRK010000193         7338 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.194, whole genome shotgun sequence.
ACCESSION   DAOTRK010000193 DAOTRK010000000
VERSION     DAOTRK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.194"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..108
                     /locus_tag="QIE41_004275"
     CDS             <1..108
                     /locus_tag="QIE41_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDS3533076.1"
                     /translation="SDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
     gene            113..856
                     /locus_tag="QIE41_004276"
     CDS             113..856
                     /locus_tag="QIE41_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3533077.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            793..1425
                     /locus_tag="QIE41_004277"
     CDS             793..1425
                     /locus_tag="QIE41_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDS3533078.1"
                     /translation="MARICLYGNLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1486..4965
                     /locus_tag="QIE41_004278"
     CDS             1486..4965
                     /locus_tag="QIE41_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDS3533079.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQMLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNHSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPML
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGRDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLI
                     VRKNGAEIYNRTALDNTLIYSGVVDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLLV
                     VVMKKATAGISIS"
     gene            5033..5632
                     /locus_tag="QIE41_004279"
     CDS             5033..5632
                     /locus_tag="QIE41_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDS3533080.1"
                     /translation="MRKLCAVILSAVVWLVAVGTPASAAEHQSTLSAGYLQAHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSMMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTYDVLNGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWHTDGFIVGVGYKF"
     gene            5697..>7338
                     /locus_tag="QIE41_004280"
     CDS             5697..>7338
                     /locus_tag="QIE41_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDS3533081.1"
                     /translation="MAAVKISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENP
                     DEAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEGSSPGTLNDFLGAMTEDDVMPE
                     ALRRFQEMVEEAARNAEAASQSAAAAKTSETAAASSKNAAKTSETNAANSAQAAAASQ
                     TASANSATAAKKSENNSKASETASKTSETNAKNSEVAAESSQSAAANSATAAKNSQDA
                     AAQSERAAASSASAAATSATASANSQRAAKTSETNAKTSETAAKTSETNAKASETAAK
                     NSQDAAAQSESAAAGSASAAAASATASANSQRAAKTSEDNAKASETTAANSAQASAAS
                     QTAAKASEDAAREYASQAAEPYKQVLQPLPDVWIPFNDSLDMITGFSPSYKKIVIGDD
                     EITMPGDKVVKFKRASKATYINKSGVLTEAAIDEPRFERDGLLIEGQRTNYMLNSESP
                     ASWGRSSNMDVPETGTDSFGFTYGKFVCNDSLIGQTSAINMASIAATKSVDVSGDNKY
                     VTTSCRFKTELQVRLRIRFDKYDGSATTFLGDAYIDTQT"
BASE COUNT         1771 a         1816 c         2229 g         1522 t
ORIGIN      
        1 tccgatatca cgaaggataa atgcagcaaa tgcctgagcg gctgtaagtt tcgcaataac
       61 gtcggcaact ttggcggctt cctttccatt aacaaacttt cgcagtaatc ccatgacaca
      121 gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt cgtgcggctt
      181 cgtggtaagc acgccggagg gggaaagata tttcccctgc gtgaatatct ccggtgagcc
      241 ggaggcgtat ttccgtatgt cgccggaaga ctggctgcag gcagaaatgc agggtgagat
      301 tgtggcgctg gtccacagcc accccggtgg tctgccctgg ctgagtgagg ccgaccggcg
      361 gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc cgggggacga ttcataagtt
      421 ccgctgtgtg ccgcatctta ccgggcggcg ctttgagcac ggtgtgacgg actgttacac
      481 cctgttccgg gatgcttatc atctggcggg gattgagatg ccggactttc atcgtgagga
      541 tgactggtgg cgtaacggcc agaatctcta tctggataat ctggaggcga cggggctgta
      601 tcaggtgccg ttgtcagcgg cacagccggg cgatgtgctg ctgtgctgtt ttggttcatc
      661 ggtgccgaat cacgccgcaa tttactgcgg cgacggcgag ctgctgcacc atattcctga
      721 acaactgagc aaacgagaga ggtacaccga caaatggcag cgacgcacac actccctctg
      781 gcgtcaccgg gcatggcgcg catctgcctt tacgggaatt tacaacgatt tggtcgccgc
      841 atcgaccttc gtgtgaaaac gggggctgaa gccatccggg cactggccac acagctcccg
      901 gcgtttcgtc agaaactgag cgacggctgg tatcaggtac ggattgccgg gcgggacgtc
      961 agcacgtccg ggttaacggc gcagttacat gagactctgc ctgatggcgc tgtgattcat
     1021 attgttccca gagtcgccgg ggccaagtca ggtggcgtat tccagattgt cctgggggct
     1081 gccgccattg ccggatcatt ctttaccgcc ggagccaccc ttgcagcatg gggggcagcc
     1141 attggggccg gtggtatgac cggcatcctg ttttctctcg gtgccagtat ggtgctcggt
     1201 ggtgtggcgc agatgctggc accgaaagcc agaactcccc gtacacagac aacggataac
     1261 ggtaagcaga acacctattt ctcctcactg gataacatgg ttgcccaggg caatgttctg
     1321 cctgttctgt acggggaaat gcgcgtgggg tcacgcgtgg tttctcagga gatcagcacg
     1381 gcagacgagg gggacggtgg tcaggttgtg gtgattggtc gttgatgaaa aacgttttat
     1441 gtgaaaccgc ctcagggcgg ttttgtcgtt tctggagcgt gaggaatggg taaaggcagc
     1501 agtaaggggc ataccccgcg cgaagcgaag gacaacctga agtccacgca gatgctgagc
     1561 gtgatagacg ccatcagtga agggccgatt gaaggtccgg tggacggatt aaaaagtgtg
     1621 ctgctgaaca gtacgccagt gctggacagt gaggggaata ccaatatctc cggtgtcacg
     1681 gtggttttcc gggcaggtga gcaggagcag acaccgccgg agggatttga atcctccggc
     1741 tccgagacgg tgctgggtac ggaagtgaag tacgacacgc cgattacccg gaccatcacg
     1801 tctgcaaaca tcgaccgtct gcgctttacc ttcggtgtgc aggcactggt ggaaaccacc
     1861 tcaaaggggg accggaatcc gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt
     1921 ggctgggtga cggaaaaaga catcaccatt aaaggcaaaa ccacctcaca gtatctggca
     1981 tcggtggtgg tgggtaacct gccgccgcgc ccgtttaata tccggatgcg caggatgacg
     2041 ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcata cactgaaatc
     2101 atcgatgtga aacagtgcta cccgaacacg gcactggtcg gcgtgcaggt ggactcggaa
     2161 cagttcggca gccagcaggt gagccgtaat tatcatcttc gcgggcgcat tctgcaggtg
     2221 ccatcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg gacgtttaaa
     2281 ccggcataca gcaacaacat ggcctggtgt ctgtgggata tgctgaccca cccgcgctac
     2341 ggcatgggga aacgtcttgg tgcggcggat gtggacaaat gggcgctgta tgtcatcggc
     2401 cagtattgcg atcagtcagt gccggatggc tttggtggta cggagccgcg catcacctgt
     2461 aatgcctggc tgaccacaca gcgtaaggcg tgggatgtgc tcagtgattt ctgttcggcg
     2521 atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga ccgaccgtcg
     2581 gataaggtgt ggacctataa ccacagtaat gtggtgatgc cggatgatgg cgcgccgttc
     2641 cgctacagct tcagcgccct gaaggaccgc cataatgccg ttgaggtgaa ctggattgac
     2701 ccgaataacg gctgggagac ggcgacagag cttgttgaag atacgcaggc cattctccgt
     2761 tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagccgggg gcaggcgcac
     2821 cgcgccgggc tgtggctgat taaaacggaa ctgctggaaa cgcagaccgt ggacttcagc
     2881 gtgggtgccg aagggcttcg ccatgtaccg ggcgatgtca ttgaaatctg tgatgatgac
     2941 tatgccggta tcagcatcgg tggtcgcgtg ctggcggtga acagccagac ccggacgctg
     3001 acgctcgacc gtgaaatcac gctgccatcc tccggtacca cgctgataag cctggttgac
     3061 gggcagggta atccggtgag cgtggaggtc cagtccgtca ccgacggcgt gaaggtaaaa
     3121 gtgagccgtg ttcctgacgg cgttgccgaa tacagcgtgt gggggctgaa gctgccgatg
     3181 ctgcgtcagc gcctgttccg ctgtgtgagt atccgtgaga acgacgacgg cacgtatgcc
     3241 atcaccgctg tgcagcatgt gccggaaaaa gaggccatcg tggataacgg ggcgcacttt
     3301 gacggcgacc agagtggcac ggtgaatggt gtcacgccgc cagcggtgca gcacctgact
     3361 gccgaagtca ccgcagacag cggggaatat caggtactgg cgcgctggga cacgccgaag
     3421 gtggtgaagg gcgtgagctt tatgcttcgc ctgaccgtgg cagcggatga cggcagtgag
     3481 cggctggtca gcacggcccg gacgacggaa accacatacc gcttcacgca actggcgttg
     3541 ggaaactaca ggctgacagt ccgggcggca aatgcctggg ggcagcaggg cgatccggcg
     3601 tcggtatcgt tccggattgc cgcaccggca gcgccgtcgc ggattgagct gacgccgggc
     3661 tattttcaga taactgccac gccgcatctt gcggtttatg atccgacggt acagtttgag
     3721 ttctggttct cggaaacgcg gattaccgat atcaggcagg ttgaaaccac agcccgctat
     3781 cttggcacgg cgctgtactg gatagccgcc agtatcaata tcaaaccggg ccgtgattat
     3841 tatttttaca tccgcagtgt gaacaccgtt ggcaaatcgg catttgtgga ggctgttggc
     3901 cagccgagtg atgatgcatc cggctatctg gattttttca aaggagagat agggaaaacc
     3961 catctggctc aggagctgtg gacgcagatt gataacggtc agcttgcgcc tgacctggct
     4021 gaaatcagga cgtccattac ggatgtcagc aatgaaatca cgcagaccgt caataagaaa
     4081 ctggaagacc agagtgcagc gatccagcag atacagaagg ttcaggttga tacaaataat
     4141 aacctgaaca gcatgtgggc tgtgaagctg cagcagatgc aggacggacg cctttatatc
     4201 gcgggtattg gtgccggtat tgaaaacacc cctgacggca tgcagagtca ggtgctgctg
     4261 gcggcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc gatgtttgtt
     4321 ggtcagggcg atcagatatt tatgaatgaa gtgttcctga aatatctgac ggctcccacc
     4381 attaccagcg gcggtaatcc tccggcattt tccctgacac cggacgggcg gctgacggca
     4441 aaaaatgccg atatcagcgg taacgtgaat gcgaactccg ggacgctcaa caacgtcacg
     4501 attaacgaga attgccgggt tctgggaaaa ctgtccgcga accagattga aggcgatctc
     4561 gttaaaacag tgggcaaagc tttcccccgt gactcccgtg caccggaacg gtggccatca
     4621 gggaccatta ccgtcagggt ttatgacgat cagccgtttg accggcagat tgttattccg
     4681 gcggtggcat tcagcggcgc taaacatgag agagagcata ctgatattta ctcctcatgc
     4741 cgtctgatag tgcggaaaaa cggtgctgaa atttataacc gtaccgcgct ggataatacg
     4801 ctgatttaca gtggcgttgt tgatatgcct gccggtcacg gtcacatgac gctggagttt
     4861 tcggtgtcag catggctggt aaatgactgg tatcccacag caagtatcag cgatttgctg
     4921 gttgtggtga tgaagaaagc caccgcaggc atcagtatca gctgaatttt ataaccccaa
     4981 tacgggcgtc agaaatgacg ccttttttat tgcagaaaag cgagaggtaa ttatgcgtaa
     5041 attatgtgct gttattctgt ccgcagtagt ctggctggtt gccgttggta cgccagcgag
     5101 cgcagcagag catcagtcca cactaagcgc cgggtatctt caggcccata ctgatatgcc
     5161 aggcagtgat gacctgaagg gcattaacgt gaaataccgt tatgaattta cggacacgct
     5221 ggggctggtg acgtcattca gttatgcagg agacaagaat cgccagatta cccgttacag
     5281 cgatacccgc tggcatgaag attcagtgcg taaccgctgg ttcagcatga tggcggggcc
     5341 gtctgtacgc gtgaatgaat ggttcagcgc gtatgcgatg gcgggcgtgg cttacagccg
     5401 tgtgtcgacc ttctccgggg attatctccg cgtaactgac aataagggga aaacgtacga
     5461 tgtgctgaac ggaagtgatg acggtcgcca cagcaacacg tctctggcgt ggggggctgg
     5521 cgtgcagttt aacccgaccg aatccgtggc cattgacctt gcttatgaag gctccggcag
     5581 tggtgactgg cacactgacg gtttcatcgt gggtgtcggt tataaattct gattagccag
     5641 gtaacacagt gttatgacag cccgccggtt caggcgggct tttttgtgga gtggatatgg
     5701 cagcagtaaa aatctcaggt gtgctgaaag atggtgcggg aaaaccaata cagaactgca
     5761 ctattcaact gaaggcaaag cgtaacagca ccacggtact ggtgaacacg gtggcctctg
     5821 aaaatcctga tgaagccggg cgttacagta tggacgttga gtatggccag tacagcgtca
     5881 tcctgctggt tgaaggtttt ccgccttcac atgccgggac cattaccgtc tatgaaggtt
     5941 ccagtccggg gacgctgaat gattttctcg gtgccatgac ggaagatgat gtcatgccgg
     6001 aggcattgcg ccgttttcag gaaatggtgg aagaagcggc acgcaacgct gaagccgcct
     6061 ctcagagcgc agcggcggca aagacatccg aaactgcagc ggcatcatcg aagaacgcgg
     6121 cgaaaacctc agaaacgaat gcagctaaca gcgcacaggc ggcagcggcc tcacagactg
     6181 catcggcaaa ctccgcgact gcagccaaaa aatcagaaaa caactcaaaa gcgagtgaga
     6241 cagcttcaaa gaccagcgaa actaatgcga aaaatagcga ggtggctgca gaatcgagtc
     6301 agagtgccgc agcaaactcc gctaccgcag caaaaaacag tcaggatgca gcagcccaaa
     6361 gcgagagagc ggcagccagt tctgcaagtg cggcggctac ttctgccacg gcatcagcca
     6421 acagtcaaag agctgcaaaa accagtgaaa ccaacgcaaa gacaagcgag actgcagcga
     6481 agaccagcga aacgaatgcc aaagccagcg aaactgcggc gaaaaacagt caggatgcag
     6541 cggcccaaag cgagagtgcc gcagctggtt ctgcaagcgc ggcggctgct tctgccacgg
     6601 catcagccaa cagtcaaaga gctgcaaaaa ccagtgaaga caacgcaaag gcgagcgaga
     6661 ctacagcggc gaactcggcg caagcatcgg cagcaagcca gacagcagct aaagcaagtg
     6721 aagacgcagc cagagagtat gcaagccagg cagcggagcc gtataaacaa gttttgcagc
     6781 cgttgcctga tgtgtggata ccgtttaacg attcactgga tatgattacg gggttttcgc
     6841 cgtcatataa aaagattgtt attggtgatg atgaaataac gatgcctggc gataaggttg
     6901 taaagtttaa acgcgcatcg aaagcaacct atattaataa atctggtgtg ctgacagagg
     6961 ctgccattga cgagccacga tttgaacgtg atggcctgct tattgagggg caaagaacga
     7021 actacatgct caattcggaa agccctgcca gttgggggcg atcgtcaaat atggatgttc
     7081 ccgaaaccgg gacggatagt tttggtttta cctatggaaa gtttgtctgt aacgattctc
     7141 tgattgggca aacctcagcc attaatatgg catcaattgc tgcaacaaag tcagttgatg
     7201 tctcaggcga taataaatac gtgacaacct catgtcgttt taaaacagaa ctgcaggtaa
     7261 ggttgcgtat ccggtttgat aaatatgacg gtagcgcaac aacttttctt ggtgatgcgt
     7321 atattgatac acaaacgc
//
LOCUS       DAOTRK010000194         7267 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.195, whole genome shotgun sequence.
ACCESSION   DAOTRK010000194 DAOTRK010000000
VERSION     DAOTRK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.195"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..110
                     /locus_tag="QIE41_004281"
     ncRNA           <1..110
                     /ncRNA_class="other"
                     /locus_tag="QIE41_004281"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(295..972)
                     /gene="narI"
                     /locus_tag="QIE41_004282"
     CDS             complement(295..972)
                     /gene="narI"
                     /locus_tag="QIE41_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDS3533082.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(972..1682)
                     /gene="narJ"
                     /locus_tag="QIE41_004283"
     CDS             complement(972..1682)
                     /gene="narJ"
                     /locus_tag="QIE41_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDS3533083.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLCARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1679..3217)
                     /gene="narH"
                     /locus_tag="QIE41_004284"
     CDS             complement(1679..3217)
                     /gene="narH"
                     /locus_tag="QIE41_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDS3533084.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3214..6957)
                     /locus_tag="QIE41_004285"
     CDS             complement(3214..6957)
                     /locus_tag="QIE41_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDS3533085.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPIEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     ILLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAKKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATFERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1636 a         2046 c         1881 g         1704 t
ORIGIN      
        1 tgagaagctt cgaccaaggt gcgactcgag cgccagcgag aaagcgttgc cgcaggcaac
       61 gacccgaagg gtgaagcgcg cagcgctgag taatccttcc cccaccacca tcacttcctg
      121 taaatactca aaacctgtta ctccgagcta attctaccca atggcaccgc gtttaccgga
      181 cctacaatat cctcccctgt aggtcggata agcgaagcgc atccgacaac tcaccccgta
      241 ccgtgcccat aaaaaaaccc cgccgaagcg gggtctatgc aaactaaaat tcgcttagtg
      301 acgagcgcgc accagctgat acttacgtgt cagatactcc accggtacgc tccagatgtg
      361 caccagacgt gagaacggga acagcaagaa taacgtcatc cccagcacca ggtgcagacg
      421 gaagataaac gccacaccat caaggtgttg agaagcgcca ccgtggaagg tcactaccga
      481 ctgcgcccag ccaaccagtt tcatcatctc gctaccgtcc atatgctggg cggagaacgg
      541 aatggtcagc aagcccagag cgcactggat aaccagcaga gacaggatca ggatatccgc
      601 tccggtagtc gttgcacgca cgcgtgggct gaacagacga cgtttcagca acagcacgcc
      661 gccaatcaga cacagcacgc cgctggcacc accagcaaac atcgccattt tctgtttcac
      721 ttcaatcggc agccaggctt catacatcca gtgcggcgtc agcataccaa agaagtgacc
      781 gacaaaaatc cccagaatac cgatatggaa caggttcgac gccaggttca tccctttgcg
      841 atccagcatc tggctggacg ccgcgcgcca ggtgtactgc ccgtagtcat aacgcagcca
      901 gctaccaatc aggaagaccg ccccggctat gtacgggtag atatcaaaga agaacatatt
      961 caggaattgc attagtgctg tcctccggtg gtgatattca gatattgcgg cgcgacggca
     1021 ccggcaaagc gacgctgatg agcagtgatt gctgaatcac cgcagccttt gtcagcaaag
     1081 aatttaacct gctcttcttc ccagacagca tccagcgcct gcggcgtatc gtcgcgcgct
     1141 tcgtcggcaa ttttttccgc cactttgtcg ctgtcgattg cggtattcgc cagtttcagc
     1201 agcagatcaa acagcacggc ataacggctt tcacgctgtt gcagacgcgc gcagagcaat
     1261 gccagaatcg gcgcgatatc tttcaaacct tccacggctt cgctttgcgg cagctgcgcc
     1321 agatactcca gatacagcgg caaatggtcg ggcaattcgc ggctgtttaa ctgcaaaccg
     1381 tgctgctcgt actgtgccag caggtccacc atcgcctgac cgcgatcgcg ggattccccg
     1441 tgaacgtgtt caaacagcaa cagcgacgtg gcgcgaccac gatcgaacag ttcgctgtac
     1501 tgcgcctggg catcgagcgg atccatcgtc gttaaatcgc gcaggaaaat gcccagcgca
     1561 tgggcatctt ctttcgacag atttttcgac gcagcaattg cctcaaacat ctcttgttga
     1621 tgctgccata aagcagcatc cggatattca aggagacgcg atacaatcac gagttcgatc
     1681 atgggtgcgg ctccgttttg ctggttacgt cgatggcatc gatacggcgg ctgttgaaca
     1741 gattgaattt ggtatctgaa ccgtggcagc catcaccaaa ggtaaagccg cagccatttt
     1801 tctccgggaa ggcttcccgc gccagttcac gatgactgct cggcaccaca aagcgatctt
     1861 cgtagttagc aatcgccaga taacggtaca tctcctgtgc ctgggcttcg gtcagaccga
     1921 cctcttccag cgcacgggta tcgactttac cgtcaacggt ttcagcacgt ttgtaatgac
     1981 gcatcgccag catacgtttc agcgcgcgca gtaccggttt ggtatcaccg gcggtcagca
     2041 gattcgccag atactgtacc ggaatacgca ggctttcgac gtctggcaga atgccgttgc
     2101 tgcccagctc acccgcgtct gctgcagact gaatcggaga cagaggcggc acgtaccaga
     2161 ccatcggcag tgtgcgatat tctggatgca gcggcagcgc cagtttccat tccatcgcca
     2221 ttttataaac cggcgactgc tgtgcggctt caataacgct cagcggaata ccgtctttaa
     2281 ttgcctgctc gatgactttc ggatcgttcg gatcgaggaa cacctccagc tgacgctggt
     2341 agagatcttt ctcgttctcg gtgctggctg cacgttcaat ggcgtcggca tcgtacaaca
     2401 gcacgccgag ataacggata cgaccgacac aggtttcaga gcacacggtc ggctgacccg
     2461 cttcaatacg cggatagcag aagatgcact tctcagactt accgctcttc cagttgaagt
     2521 agattttttt gtatgggcat ccggtgatgc acatacgcca gccacggcat ttatcctggt
     2581 cgatcaggac gatgccatct tcttcacgct tgtaaatcgc accgctcggg caggtcgcca
     2641 cacatgccgg gttcaggcag tgttcgcaca ggcgcggcaa atacatcatg aaggtgtttt
     2701 cgaactggct atacatcgcc ttctggatgt tgtcgaagtt cttgtctttc gccagcttgt
     2761 caaactcacc gcccaggtca tcttcccagt tcggcccttt ttcgattttc gccatccgtt
     2821 ccccggtaat cagcgaacgc ggacgggcaa tcggctgcga tttgctgcct tccggcgcgg
     2881 tatgcaggtt ctgataatca aaatcgaacg gctcgtaata atcgtcgatc cccggcagat
     2941 gcgggttagc gaagatttta cccagcagca tggcgcggtt acccatgcgc ggttgcagtt
     3001 tgccattgat tttacggatc cagccgcctt tgtatttttc ctggttttcc cagtcagtcg
     3061 ggaagccctg gcccggcttg gtttccacgt tgttgaacca cgcgtattcc acgccttcgc
     3121 ggctggtcca gacgttttta caggtaactg aacaggtgtg gcagccgatg cacttatcga
     3181 gattcagcac catgccgact tgtgaacgaa ttttcatttt acgctctcct gtacctggtc
     3241 attgccttcg ccatctaacc agtcaatgtt cttcatctta cgcactacaa caaactcatc
     3301 gcggttagaa cctacggtgc catagtagtt aaagccgtat gccagatggg catagccgcc
     3361 gatcatatgc gtcggtttcg gcgtgatacg ggtgaccgaa ttatggatac caccacgctg
     3421 ttgggtaatt tccgaaccag gcaggttaac gatacgttcc tgcgcgtggt acatcatggt
     3481 catccctgcc ggaacacgct ggctgacaac cgcacgggca gtcagtgcac cgttgctgtt
     3541 gaagacttca atccagtcgt tatcggcgat acccagctct ttggcatcgg cttcactcag
     3601 ccagaccacc ggaccaccgc gacctaaagt cagcatcagc aggttgtcgc tgtaggtgga
     3661 gtggataccc cacttctggt gcggcgtcag gaagttgagc gctttttccg ggttgccgtt
     3721 ggatttctgg cccatcactt ctttcaccga acgggtgtcg atcggcggac gataaaccag
     3781 caggctttca ccgaaatcac gcatccactg gtgatcctga tacagctgct gacggccaga
     3841 gagcgtacgc cacgggatca gctcgtgaac gttggtgtaa ccggcgttgt aagaaacgtg
     3901 ttcatcttca aggccagacc aggtcgggct ggagatgatt ttgcgcggct gtgcctgaat
     3961 atcgcggaag cgaatcttct cgtcttcttt attcagcgcc agatgcgtat ggtcacgacc
     4021 ggtaaactcg ctcagggcag cccaggcttt tacagctacc tgaccattgg tttccggtgc
     4081 cagcgtcagg atcatctctg ccgcatcaat tgcggtattg agcatcggct gaccttttgc
     4141 cggaccttcc gctttggtgt agttgagctt acgcagcaga tccatctcgc tctgggtgtt
     4201 ccaggcaatc cctttaccgc cgttaccgat tttctccatc agcgggccga tagaggtaaa
     4261 gcgttcgaaa gttgccggat aatcgcgctc tacgaccata atgtgcggtg cagttttacc
     4321 tgggatcaga tcgcattcgc cttttttcca gtctttcaca tccagcggtt gcgccagttc
     4381 agcggcagag tcgtgctgga taggcagcgt gacgatgtca gtttctttac ccagatgacc
     4441 aacgcacact tcggagaatt tcttcgcgat ggctttgtag atttcccagt cgcttttcgc
     4501 ttcccaggcc ggatcgaccg ccgcagacag cgggtgaata aacggatgca tatccgaagt
     4561 attcatgtcg tctttttcgt accaggtcgc cgtcggcaaa atgatgtcgg aatagagaca
     4621 ggtgctcgac agacggaagt ccagcgtcac caccagatcc agcttgcctt ccagaccgtt
     4681 gtcctgccag tccacttctt ccggcttcac gccgccctgc tgccccaggt ctttgccctg
     4741 gataccgtgc tccgtcccca gcaggtactt gagcataaac tcatgacctt taccggaaga
     4801 accgagcagg ttagaacgcc agatgaacag gttacgcggg tggtttttac cgttttctgg
     4861 ttgttctgcc gcaaaacgga tagaaccctc tttcagggat ttcacggtgt aatccaccgg
     4921 attcatgccc gcttttttcg cttcgccagc gatagttagc gggttagtgc ctaactgcgg
     4981 cgcagacggc agccagccca tacgttctgc acggacgtta aagtcgatca agtgtccggt
     5041 ataacgggat ttatccgcca tcggcgacaa caactcttct gcggtcacgg tttcataacg
     5101 ccactggctg gagtggttat agaaataaga ggtgctgttc atgtgacgcg ccggacgctg
     5161 ccagtcaaga gcaaacgcca gcggctgcca gccggtttgc ggacgcagtt tttcctgacc
     5221 tacatagtgc gcccagccac ccccgctctg accgacacag ccgcagaaaa tcagcatgtt
     5281 gatcagacca cgatagttca tatcgaggtg ataccagtgg ttcagccccg caccgacgat
     5341 aatcatcgaa cgaccgtgcg tcttgtcagc gttatcggca aattcacggg caatacggat
     5401 aatctggcta cgagaaacgc cagtgatttg ctcggcccac gccggagtgt aagctttcac
     5461 atcgtcatag ctggttgcgc agttaacgtc gttcaggcca cgttccagac cgtagtttgc
     5521 cagtgtcaga tcataaacgg tggtcaccag tgcggtgctg ccatcagcca gttgcaggcg
     5581 ttttaccggc agtttgtgca gcagaatgtt ttccagctcc actttgttga agtgttccgt
     5641 gccgtcgcca ccaaagtacg ggaagcccac ctcagcgatc tcatcctggc tacccagcag
     5701 actcagttgc agctcggttt cttcgccagt tttgccgtcg cgctgctcaa gattccattt
     5761 gcccttctcg ccccagcgga agccaataga accgttcggc gcaaccattt cgccattggt
     5821 attaaaggcg acagttttcc attccggatt gttttcctgg cccagcgcat ccaccagatc
     5881 agcagcacgc agcatacgac ctgcggcgta gtaaccgtcg cgttcttcca gcatcaccag
     5941 catcggcatg tcggtgtagc gacgcacata gtcggtgaaa tactggcttg ggttgtcgag
     6001 gtggaattca cgcagcatta cgtggcccat cgccagcgcc attgctgcgt cggtgccctg
     6061 tttcggtgcc agccacagat cgcacagttt ggcgatttca gcgtagtccg gtgtgacggc
     6121 aacagttttg gtgcctttgt aacgcacttc agtaaagaag tgagcatccg gagtacgcgt
     6181 ctgcggcacg tttgaacccc aggcgatgat gtagctggag ttgtaccagt cagcagattc
     6241 cggtacgtca gtctgctcgc cccaggtttg cggagacgca ggaggcaggt cgcagtacca
     6301 gtcgtagaag cttaagcaag taccgccaag cagcgagaga taacgcgcac ccgacgcgta
     6361 agaaaccatc gacattgccg gaattggcga gaagccagcg acacggtccg ggccgtagtt
     6421 tttgatggtg taaacgttag atgcggcgat cagttcgttc acttcctgcc aggaagaacg
     6481 aacaaaacca ccgcgtccac gcgcctgctt aaagcttttc gctttatcgg cgtcttcaat
     6541 gatagaagcc catgcctcaa tcggatcgct atgcagcgcc ttcgcttcac gccacatttt
     6601 catcaggcgt ttgcgcatca tcgggtattt caggcggttg gcactgtaaa gataccagga
     6661 gtagctggca ccgcgcgggc agccgcgagg ttcatggttt ggcagatccg gacgggtacg
     6721 aggatagtca gtctgctggg tttcccaggt caccagaccg tttttgacgt agattttcca
     6781 gctgcaggag ccggtgcagt ttaccccgtg ggtagagcgg acgattttgt catgctgcca
     6841 acgctggcga tatccatcct cccagtcacg gttggtattg agaagctggc catgcccatc
     6901 ggcaaaggtt tcacccttct gtttgaagta gcgaaaccgg tccaggaatt tactcatcgg
     6961 ttttctcctg tgggagcctg tcggccctct gataaatcga cattgctaat gtttaagggc
     7021 aaggtaacgc tctgaaacag cgtgagaatt gataacgatc aagagtgatg gggatgaaaa
     7081 ataaagtaaa ttcctaagat acccctttag gataattaac agccacaaca taacagactg
     7141 taaattaaga cgttttatac ctgtagttat ttaaactatt aagattttta tgcagatggg
     7201 tattaaggag tattccccat gggtaacata ttaatggctc ttaatgaaat ccggcgaaat
     7261 aagccac
//
LOCUS       DAOTRK010000195         7176 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.196, whole genome shotgun sequence.
ACCESSION   DAOTRK010000195 DAOTRK010000000
VERSION     DAOTRK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.196"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..460
                     /locus_tag="QIE41_004286"
     CDS             <1..460
                     /locus_tag="QIE41_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDS3533086.1"
                     /translation="GFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPS
                     GTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKS
                     WKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            complement(505..708)
                     /locus_tag="QIE41_004287"
                     /pseudo
     CDS             complement(505..708)
                     /locus_tag="QIE41_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1181..1726)
                     /gene="nfaA"
                     /locus_tag="QIE41_004288"
     CDS             complement(1181..1726)
                     /gene="nfaA"
                     /locus_tag="QIE41_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021566166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr family non-fimbrial adhesin I structural
                     subunit NfaA"
                     /protein_id="HDS3533087.1"
                     /translation="MKIKYTMKMAAVASVMVAGYAIADANGLNTVNAGDGKNLGTATA
                     TATITTLQSCSVDLNLVTPNATVNRAGMLANREITKFSVGSKDCPSDTYAVWFKEIDG
                     EGQGVAQGTTVTNKFYLKMTSADGTASVGDINIGTKSGKGLSGQLVGGKFDGKITVAY
                     DSATAPADVYTYDLMAAVYVQ"
     gene            complement(1927..2100)
                     /locus_tag="QIE41_004289"
     CDS             complement(1927..2100)
                     /locus_tag="QIE41_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DraP"
                     /protein_id="HDS3533088.1"
                     /translation="MGNLPVGSRGLYKGKKRSVGMNELRHPGSTQMTRTGQAHRGGEH
                     RLNTGPPDWQTVE"
     gene            complement(2479..2922)
                     /gene="draD"
                     /locus_tag="QIE41_004290"
     CDS             complement(2479..2922)
                     /gene="draD"
                     /locus_tag="QIE41_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr fimbrial tip invasin DraD"
                     /protein_id="HDS3533089.1"
                     /translation="MNGSIRKMMRVTCGMLLMVMSGVSQAAELHLESRGGSGTQLRDG
                     AKVATGRIICREAHTGFHVWMNERQVDGRAERYVVQSKDGRHELRVRTGGDGWSPVKG
                     EGGKGVSRPGQEEQVFFDVMADGNQEIAPGEYRFSVGGACVVPQE"
     gene            complement(2938..5520)
                     /gene="afaC"
                     /locus_tag="QIE41_004291"
     CDS             complement(2938..5520)
                     /gene="afaC"
                     /locus_tag="QIE41_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153002854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfaC-I/III family usher protein"
                     /protein_id="HDS3533090.1"
                     /translation="MRDTSSGRMRTGVTGLALAVMVACVMFRAESGIARTYSFDAAML
                     KGGGKGVDLTLFEEGGQLPGIYPVDIILNGSRVDSQEMTFHAERDAEGRPYLKTCLTR
                     EMLARYGVRIEEYPALFRVSGEGRGASVAEEACADLTAIPQATESYQFAAQQLVLGIP
                     QVALRPQLRGIAPEALWDDGIPAFLLNWQANAGRSEYRGYGKRVTDSYWVSLQPGINI
                     GPWRVRNLTTWNRSSGQSGKWESSYIRAERGLNGIKSRLTLGEDYTPSDIFDSVPFRG
                     AMMSSDESMVPYNLREFAPVVRGIARTQARIEVRQNGYLIQSQTVAPGAFALTDLPVT
                     GSGSDLQVTVLESDGTAQVFTVPFTTPAIALREGYLKYNVTAGQYRSPDDAVEQTSLG
                     QVTAMYGLPWGLTVYGGVQGAEHYQAAALGLGWSLGRLGAVSLDTTHSRGQQKGHDYE
                     TGDTWRIRYNKSFELTGTSFTAASYQYSSDGYHTLPDVLDTWRDDRYTYRHTENRSRR
                     TTLSLSQSLGQWGYVGLNGSRDEYRDRPHRDYFGASYSTSWNNISLSVNWSRNRNSGG
                     YYGGWSRTEDSVSMWMSVPLGRWFGGTDNDISATAQMQRSTGQDTRYEAGLNGRAFDR
                     RLYWDVREQMVPGSESHADTSRLNLTWYGTYGELTGMYSYSSTMRQLNAGMSGSMVAH
                     SEGVTFGQRSGDTVALIAAPGVSGASVGGWPGVRTDFRGYTLAGYASPYQENVLTLDP
                     TTFPEDAEVPQTDSRVVPTKGAVVRAGFRTRVGGRALVSLARQDGTPLPFGAVVTVEG
                     EAGQAAGSAGVVGDRGEVYLSGLKESGKLKAQWGENSLCHADYRLPEEKGPAGIFLTR
                     TVCM"
     gene            complement(5560..6357)
                     /gene="draB"
                     /locus_tag="QIE41_004292"
     CDS             complement(5560..6357)
                     /gene="draB"
                     /locus_tag="QIE41_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021517431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr fimbrial biogenesis chaperone DraB"
                     /protein_id="HDS3533091.1"
                     /translation="MKMRAVAVFTGMLTGVLSVTGLLSAGAYAAGGEGNMSASATETN
                     ARVFSLHLGATRVVYNPASSGETLTVINDQDYPMLVQSEVLSEDQKSPAPFVVTPPLF
                     RLDGQQSSRLRIVRTGGEFPSDRESLQWICVKGIPPKEGDRWAEGKDGEKKADKVTLN
                     VQLSVSSCIKLFVRPPAVKGRPDDVAGKVEWQRAGNRLKGVNPTPFYINLSALTVGGK
                     EVKEHEYIAPFSSREYPLPAGASGKVQWKVITDYGGTSKQFEAELKG"
     gene            complement(6625..6930)
                     /gene="afaA"
                     /locus_tag="QIE41_004293"
     CDS             complement(6625..6930)
                     /gene="afaA"
                     /locus_tag="QIE41_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFA-III adhesin operon transcriptional regulator
                     AfaA"
                     /protein_id="HDS3533092.1"
                     /translation="MRERYLYLADTPQGILMSGQVPEYQFWLLAEISPVHSEKVINAL
                     RDYLVMGYNRMEACGRHSVSPGYFSGALKRFQRVSQTVYRLVPFYFPEAGHEVHRGE"
BASE COUNT         1564 a         2316 c         1612 g         1684 t
ORIGIN      
        1 gggcttcgtc tatgtggcgt tcatcattga tgtgtttgcc ggatacatcg tggggtggcg
       61 ggtctcatcg tccatggaaa cgacattcgt gctggatgca ctggagcagg cgttatgggc
      121 ccgtcgaccg tccggcacgg tccatcacag tgataaaggt tctcagtatg tatcgctggc
      181 ctacacacag cggcttaagg aagccggatt actggcatca acaggaagta caggcgactc
      241 gtatgacaac gcgatggcgg agagcatcaa tggtctttac aaagcggagg taatacaccg
      301 taagagctgg aaaaaccgtg cagaagtgga actggccaca ctcacgtggg tggactggta
      361 taacaatcga cgattgctgg aaaggctggg ccatactcct ccggcagaag cagaaaaagc
      421 ttattatgct tccatcggaa acgatgatct ggcagcctga gttcacagat aaaacactct
      481 ccaggaaacc cggggcggtt catgcagaat ttcagattcc agttcagcca ccgtgcggga
      541 accaggagta ccgagccctt ttctggcggc ggtaacccat tgtcctaaag tgccttcagg
      601 aagagataat cgggaagcgc cttcgctgat cgaaagttga ttttcaagaa ccgttctgac
      661 agcttcggct ttgaactctt tagagtaacg ttgggttttt ctgctcatta ttagctcctt
      721 ctgatgccat tctatttcag gaaggagtgt ccgttaaact caggctacct cagccttcgc
      781 atgtcacata atgaaagtgt gccgtaagga ctgcgatatt tatccaaata gcgcacatca
      841 atagcgtgtc agggtaggcg ggcttctatt gtttagcgcg ggttatcgaa agtcgttccg
      901 tgtttttggg ttcttgagtt tttccgctcg cacagcgaga cccgggcgtc ggtgtgcgat
      961 cgtcgtacgt tcggtcagga cgaccagatg tagaagtgta cttgcgtgtt ccatatgtcc
     1021 tgttaatgct gataccggcg gtactggcat ggaaacggag cgatcgcacc ccgccgcccg
     1081 tagcagttct gacactaggt tgcctgcgac gaggtcgatt cccgcgagga aagcaagacc
     1141 gccggcgggc ggcggtcagt gttagttatg tgggtaagca ttattggacg tacacggcgg
     1201 ccatgagatc gtatgtataa acgtcggcgg gggcggtggc cgagtcatat gccaccgtta
     1261 tttttccgtc gaattttccc cctaccagtt gaccactcag gcctttgcct gatttggttc
     1321 ctatgttgat gtcccctacg ctcgcggtcc cgtcggccga tgtcatttta aggtaaaact
     1381 tgttggtcac cgtagtgccc tgcgcgaccc cctgtccttc gccatcgatc tctttaaacc
     1441 atacagcata ggtgtcgcta gggcaatcct tactccctac cgaaaattta gtgatttcgc
     1501 ggtttgctag cattcctgct ctgttcactg tcgcgttcgg ggtaacgaga ttcaggtcga
     1561 cagagcagct ctgcagagtg gtgatcgtcg cggttgcggt tgcggtgccc agattcttgc
     1621 catccccggc gttcacagtg ttgagcccat ttgcatccgc gatagcgtag ccggcgacca
     1681 tgacgctggc aacggcggcc attttcattg tatatttgat tttcatattt ttttgccttc
     1741 gttttgctta tggacaagcc agttgtgttc tgttaatcca tcccagaatc agtaaactgt
     1801 cttaccccac attaaccacc gttcacagat gtctgcaccc gcagcagaga ctgttcattg
     1861 catataagta agtttctgac tgcagggctt gctcataaag ccgcagacaa cgttcacggt
     1921 gatgccttat tccacggtct gccagtccgg tggccccgtg ttcagccggt gttcgccacc
     1981 acgatgcgcc tgaccagtgc gcgtcatctg tgtgcttccc ggatgacgta attcattcat
     2041 accgacgctg cgttttttac ctttatataa accacgactt cccactggca gattacccat
     2101 aataaaacct ccaccttatt tctgatttaa atgattctga ctgtttaaat taaatccgga
     2161 tggccgattt atatttatgt aataaaccgc cagcacggat taactgcctg tgttaatcag
     2221 ctttgtgctt tccttctctg gtcttttatt tttcctttat tctttattct ttcctgtatt
     2281 ccctgcgtgc cccctccggg ttgcaggtat ccctccacct cttctgcgga atacaagtca
     2341 cggtccatgc gtgaatcagt gtttatttca tttcagttaa tttatccggt gaaataaata
     2401 ctctctgtgt tttctgcttt gtttttgttt tatgctgtgt tgtgcttttg tctttttctt
     2461 ctgtttttct tctgcgcttt attcctgtgg caccacacag gctccgccaa ccgaaaaccg
     2521 gtattcacca ggagcaattt cctgatttcc gtccgccatc acatcaaaaa aaacctgctc
     2581 ctcctgaccg ggcctcgaca cccccttccc gccttctccc ttcaccggcg accagccatc
     2641 tcctcctgtc ctgacacgaa gctcatgacg accatcctta ctctgcacca catagcgctc
     2701 cgccctgccg tccacctgac gctcattcat ccacacatga aaacccgtgt gcgcctcccg
     2761 gcagataatc cgccccgtcg ccaccttcgc accatcgcgc agctgcgttc ctgaacctcc
     2821 ccggctctcc aggtggagct cagccgcctg cgacacacca ctcatgacca tcagtaacat
     2881 cccgcaggtg acacgcatca tcttccttat actcccgttc atctccggct cctcccgtca
     2941 catacacacc gtacgggtca ggaatatccc cgcaggcccc ttctcttccg gaagacggta
     3001 atccgcatgg cacagactgt tctctcccca ctgcgccttc agcttaccgc tttccttcag
     3061 cccgctcagg tacacctcac cacggtctcc caccacaccg gctgagcccg cagcctgacc
     3121 cgcttcgccc tcaactgtca ccaccgcacc aaacggcagc ggcgttccgt cctgacgggc
     3181 cagactcacc agcgcacgac cacccacacg ggtcctgaat ccggcccgga ccactgcccc
     3241 cttcgtcggc accacacgac tgtccgtctg cggcacttcc gcatcctccg ggaacgtcgt
     3301 cgggtccagt gtcagcacgt tctcctggta cggcgaggca taaccggcca gcgtataccc
     3361 ccggaaatcc gttctcacac ccggccagcc acccacagac gcaccactca cgcccggtgc
     3421 cgcaatcagt gccaccgtat ccccgctccg ctgaccaaag gtgaccccct cactgtgggc
     3481 caccatgctg ccggacatcc cggcgttcag ctggcgcatc gtgctgctgt aactgtacat
     3541 ccccgtcagt tcaccatatg tcccgtacca cgtcaggttc agacgactgg tgtcagcatg
     3601 gctctcactg cccggcacca tctgctcacg gacatcccag tacagccggc ggtcaaatgc
     3661 ccgtccgttc agcccggcct cataccgggt atcctgtccc gtggaacgct gcatctgcgc
     3721 cgtggcactg atgtcgttat ccgtcccccc aaaccagcgt cccagcggca cactcatcca
     3781 catactgaca ctgtcttccg tacgcgacca gccaccgtaa tagccgccgc tgttgcggtt
     3841 gcgtgaccag ttaaccgaca gcgagatatt gttccaggac gtactgtatg acgcgccaaa
     3901 ataatcacgg tgcggtctgt cacggtactc atcccggctg ccgttcagtc ccacatagcc
     3961 ccactgaccc agggactgac tcagactcag cgtggtacgg cgactccggt tctccgtgtg
     4021 acggtatgtg taccggtcat cacgccaggt gtccagcacg tccggcagcg tatggtaacc
     4081 atccgatgag tactgataac tcgctgccgt aaaactcgtc cccgtcagct caaacgactt
     4141 gttataacgg atacgccagg tgtcaccggt ctcataatca tgtcccttct gctgcccccg
     4201 ggagtgcgtc gtgtccagcg acaccgcccc cagacgcccc agtgaccagc caagccccag
     4261 ggccgcagcc tgataatgct ccgcaccctg aacacccccg tacaccgtca gcccccacgg
     4321 cagaccgtac atggccgtca cctgtcccag cgacgtctgc tcaaccgcat catccggtga
     4381 acggtactga cccgccgtga cgttgtactt caggtacccc tcacgcagcg caatggccgg
     4441 cgtggtgaac ggcaccgtga aaacctgcgc cgtcccgtct gattccagca ccgtcacctg
     4501 caggtcactg ccggaccccg tcaccggcag gtccgtcagg gcaaatgccc ccggcgccac
     4561 cgtctgactc tgtatcagat acccgttctg acgcacctct atcctggcct gcgtgcgggc
     4621 aatgccacgt acaaccggcg caaattcacg caggttataa ggcaccatgc tctcatcaga
     4681 actcatcatc gcaccccgga aaggcacact gtcaaaaatg tctgacggcg tgtaatcctc
     4741 acccagcgtc aggcgactct ttatcccgtt cagcccccgc tcagcacgta tgtatgaact
     4801 ctcccatttt cccgactgac cggatgacct gttccaggtg gtcaggttcc tcacacgcca
     4861 gggtccaatg ttgattcccg gctgcagact gacccagtaa ctgtccgtga cacgcttccc
     4921 gtaaccccgg tactcactgc gccccgcatt cgcctgccag ttcagcagaa aagccggaat
     4981 gccatcatcc cataacgcct ccggcgcaat ccccctcagc tgcggacgca gtgccacctg
     5041 agggataccc agaaccagct gctgggcagc aaactgataa ctctccgtgg cctgcggtat
     5101 cgccgtcagg tcagcacagg cctcctccgc cacggatgca ccacgaccct ctccggatac
     5161 acggaacaac gccggatatt cctcaatcct gaccccgtaa cgcgccagca tctcacgggt
     5221 cagacaggtc ttcagataag gcctgccctc cgcgtccctc tccgcgtgaa aggtcatctc
     5281 ctgtgaatcc acacgggaac cattcaggat aatgtcaacc ggataaatgc cgggtaactg
     5341 cccaccttcc tcaaacaggg tcaggtccac ccccttcccg ccacctttca gcatggccgc
     5401 atcaaaggag taggtgcgcg caataccact ctccgcacga aacatcacac aggccaccat
     5461 cacagccagc gccagccctg tcacccccgt tctcatccgc cctgaagaag tatcacgcat
     5521 ctgtcagccc gccatttacc ctgttatcac cttatgtatt caacccttca gctctgcctc
     5581 aaactgctta ctggtcccgc cgtaatccgt tatcaccttc cactgaacct tacccgatgc
     5641 ccccgcaggc agcggatatt cacgggagga aaacggcgca atatattcat gctccttcac
     5701 ttccttaccc cccaccgtca gcgcggacag gttgatgtaa aacggcgtcg ggttaacccc
     5761 cttcagcctg ttgccggctc tctgccactc caccttaccg gccacatcat ccggtcgccc
     5821 cttcaccgcc ggcggacgaa caaacagctt gatgcagctg ctcactgaaa gctgtacatt
     5881 cagagtgact ttgtcagcct tcttctcccc gtccttccct tccgcccacc tgtcaccctc
     5941 cttcggcgga atgcctttca cgcaaatcca ctgcagactc tcacggtctg acggaaactc
     6001 cccgcccgtt ctaacaatac gcagacgact cgactgctga ccatcaagac ggaacaacgg
     6061 cggtgtcacc acaaaaggcg ccggactctt ctggtcctca ctcagcacct ccgactgcac
     6121 cagcatcgga tagtcctggt cattaatcac cgtcagcgtc tcccccgacg aggccgggtt
     6181 gtaaaccacc cgcgtggccc ccagatgcag cgagaatact ctggcgtttg tctccgtcgc
     6241 ggatgcagac atattccctt ctcccccggc ggcatatgcc cccgctgaca gcaaacctgt
     6301 cactgataac actcccgtca gcatgccagt gaacaccgcc acagcccgca ttttcatact
     6361 ccctccggaa cagacagata cacgttgtct tttatatatt cattcaccat ccccacccgg
     6421 aactgactct gaccggactg catgcagtca ccgcattcgt gccgggtggc gaaacggatg
     6481 gtgaaactga tgttaatatt cagtgcttaa tgcttaatgc ttaatgctta atgcttaatg
     6541 cttaatgctt aatgcttaac agaaatagct ttaattagcg taataatatc aaccgcactt
     6601 attaaacccg ctcacgccct ttcatcactc tcccctgtga acttcatgac ccgcctccgg
     6661 gaaataaaaa ggcaccagcc tgtataccgt ctgactgacc cgctgaaacc gcttcagtgc
     6721 accagaaaaa tatcccggcg acacactatg acgcccgcag gcctccatgc ggttatatcc
     6781 cattaccaga taatccctca gcgcattaat aaccttctca ctgtgtaccg gcgatatctc
     6841 agccagtaac cagaactgat attccggcac ctgaccggac atcagtatcc cctgaggggt
     6901 gtcagcaaga tacagatatc gctccctcat ctctgattca ctccatgcac acgaataaca
     6961 tgcagggaat gaagcctttt cacttcagat tcaacaggat acctgtctcc cccctgcgtc
     7021 aacggcacaa atactaccat ggtgtttttt acttaaaaat atacattgca cgatcttttt
     7081 atctacaaaa taacaaaaca ccagaattaa ataacacaaa aaaagaatac aatcagaata
     7141 tatgactgga atattattca tatgcagaat aaaaga
//
LOCUS       DAOTRK010000196         7160 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.197, whole genome shotgun sequence.
ACCESSION   DAOTRK010000196 DAOTRK010000000
VERSION     DAOTRK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.197"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            161..1555
                     /locus_tag="QIE41_004294"
     CDS             161..1555
                     /locus_tag="QIE41_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDS3533093.1"
                     /translation="MNTHSSTVSLTDNQQAAQERLETSEGRREFWRATVSCWLGTTME
                     YADFALYGLAAGIIFGDVFFPEATPVMALLSSFAAYSVGFIARPIGALLFGWIGDKHG
                     RKIVMVITIGLMGMSTMLIGLIPSYAQIGVWAPICLVILRFSQGLGAGAELSGGTVML
                     GEYAPVKRRGLVSSVIGLGSNSGTLLASLVWLIVLQMDKDDLLSWGWRIPFLCSILIA
                     AAALLIRRHIRETPVFERQKALLQAEREKVIREEKAQQQHDSRSFWKRTRAFWTMVGL
                     RIGENGPSYLAQGFIIGYVAKVLMVDKSVPTAAVLIASVLGFAIIPLAGWLSDRFGRR
                     IIYRWFCLLLILYAFPAFMLLDSREPWIVIPTIITGMGLASLGIFGVQAAWGVELFGV
                     TNRYTKMAFAKELGSILSGGTAPLIASALLSYYGHWWPIAIYFAFMAAIGLVTTFFAP
                     ETRGRDLNLPEDAI"
     gene            1660..2691
                     /locus_tag="QIE41_004295"
     CDS             1660..2691
                     /locus_tag="QIE41_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDS3533094.1"
                     /translation="MTQSHSQRVTRSDVAKEAGTSVAVVSYVINNGPRPVAEATRQRV
                     LQAIKKTGYRPNGIARALASGSTQTYGLVAPDISNPFIASMAHALQHEAFADGKVLLL
                     GDAGDSSCRERELINNMLHRQVDGLIYTSVDRHPYINLIQESGTPCVMLDRVDPGLNV
                     SVIQVDEQLAAMQVTQHLIDHGYRDIGIICGPREMLNTQDRIRGWQQALEASSLVVNP
                     SWIFSTNYTRAGGYEATKRMLEHQLPRALFATNEQQALGCLRALAEHGLRVPEDVALV
                     CFNATQESAYNVPSLTAVRQPVDKMARAAIDMLKNWDGEVRRVEFEFYLRTGESCGCQ
                     GHEVQPEKK"
     gene            2666..3685
                     /locus_tag="QIE41_004296"
     CDS             2666..3685
                     /locus_tag="QIE41_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDS3533095.1"
                     /translation="MKFNPRKSENESMRIIIDCDPGNGIPGANIDDGLALALAIAAPQ
                     IALEMITTVAGNTPVDVGYAVARDLITQLDIPVAVYRGASRALLEDPQPWREKLDHGV
                     DQFGLRQLWSNVPAPALCQQVEPHAPEAIGELICRNPGEITLVATGPLTNVAIALQLY
                     PQIVHAVKNIVVMGGVFNVPGYLKDTNFGLDPEAAHAVLTSGAPVTLVPMDVTTQTQM
                     LHADLDRLAKTENGLSRYLAQTIRPWITYSMQTRNLPGCWIHDVLTIAWLLDPSLATT
                     AEDYLDVSLEGITRGMTCCYGRDTLRLNIGIPEPKGAQVTILQSIDNPRLISLIEHYI
                     QNYGA"
     gene            3749..4462
                     /locus_tag="QIE41_004297"
     CDS             3749..4462
                     /locus_tag="QIE41_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDS3533096.1"
                     /translation="MNTLSFLLFSEEESLFITLGKICVAFILGGIIGLERESKGKPVG
                     FKTCVIISVASCVLTIVSIQSAEYYAEISMNIRSDPMRLAAQIISGVGFLGAGVILHR
                     HDDAISGLTTAAIVWASAGVGITCGAGFYMHALVTTALFMIAIKLSYFVLFLQAKNQF
                     PGKVKVHVTLDERSGLQALIECINQQKNIIEAFTIRDVKKGRIEVNLKVVIRKKMTLP
                     ELYHSLSKLEHVCAIALEH"
     gene            complement(4619..5992)
                     /locus_tag="QIE41_004298"
                     /pseudo
     CDS             complement(4619..5992)
                     /locus_tag="QIE41_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(6038..7031)
                     /locus_tag="QIE41_004299"
                     /pseudo
     CDS             complement(6038..7031)
                     /locus_tag="QIE41_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1678 a         1783 c         1777 g         1922 t
ORIGIN      
        1 accttatgag tgatctccat cacgatttta cttccattga atggtttatc tacgcgagta
       61 gatttattta tatgtaataa aacacaatat ctacatgagt agatttaaga aagatatcta
      121 cctgtgatgt atatctgacg gataatatca aggagttctt atgaacacgc attcatccac
      181 tgtttccctg acagataacc aacaggcagc tcaggagcgg ctggaaacct ccgagggacg
      241 ccgcgagttt tggcgagcaa cagtttcctg ctggttaggc accacgatgg aatatgccga
      301 ttttgccctg tacggccttg ccgcaggtat tatctttggc gatgtctttt ttccggaggc
      361 gacacccgtc atggcattac tttccagctt tgccgcctat tctgttgggt ttattgcccg
      421 ccctattggt gcattactat tcggctggat aggcgataaa catggtcgga aaattgtcat
      481 ggttatcact attggattga tgggcatgtc gaccatgcta ataggattaa tccccagtta
      541 cgcccagata ggcgtctggg caccgatatg tctggttatc cttcgatttt ctcaggggct
      601 gggagcagga gcggaacttt caggcggtac tgtgatgctt ggtgaatatg ctcccgttaa
      661 acgacgtgga ctggtttcat ctgttattgg tctgggttcg aacagcggaa cattactggc
      721 ttcgctggtt tggctcatcg tcctgcaaat ggacaaggat gacttattaa gctggggatg
      781 gcgtattcct tttctttgca gcattcttat tgctgctgca gctctattaa ttcgtcgtca
      841 tatacgcgaa acaccagtct ttgaacgtca aaaagccctt ctgcaggctg aacgagaaaa
      901 ggttattcgt gaggaaaaag cacagcaaca acatgacagt cgtagcttct ggaaacggac
      961 ccgtgccttc tggaccatgg tcggattacg cataggagag aatggtcctt cttatctcgc
     1021 tcagggattt atcattggct atgtcgcgaa agtactgatg gtggataagt ccgtacccac
     1081 ggcagctgta cttattgcat ccgttctggg atttgccatt attcctctgg cgggttggct
     1141 gtccgataga ttcggtagac gtatcatcta tcgttggttc tgcttgttac tgatcctgta
     1201 tgcctttccg gcatttatgt tgctggattc tcgtgagccg tggattgtta tcccgacgat
     1261 cattaccggg atggggctgg cttcactggg tatttttggt gttcaggctg cgtggggcgt
     1321 tgagcttttc ggtgtcacta atcgttatac caaaatggca tttgcaaaag agctcggttc
     1381 cattctgtct ggcgggactg caccacttat cgcctctgcg ctactctcgt attacgggca
     1441 ctggtggcca atcgctatct atttcgcctt tatggccgcg attggactgg tgaccacttt
     1501 ctttgcacca gagactcgcg gacgggatct caacttaccc gaggatgcaa tttaaggcca
     1561 gagaacgtga gatgcgcgtt ctttaccaaa gatttttggg tcataacgcc ttatgctgtc
     1621 tggaaaaaat cactctgaac gcaatcaggt aggtgcttcg tgactcaatc acattcacaa
     1681 cgcgtaacac gttctgacgt ggcaaaagaa gcagggacat ccgtcgctgt cgttagttac
     1741 gttattaata atggcccgcg ccccgttgcc gaagcgaccc gacaacgtgt actacaggcc
     1801 attaagaaaa ccggttatcg acccaatggc atcgcgcgtg cgctggcttc agggagtacg
     1861 caaacttatg ggctagtcgc tccggacatc tcgaacccgt ttatcgcctc tatggctcat
     1921 gccctgcaac acgaagcctt tgctgatggc aaagtactcc tgctgggcga cgcaggcgat
     1981 agtagctgcc gtgaacgtga actcattaat aatatgctac accgccaggt tgacgggctg
     2041 atctacacca gtgttgatcg ccatccttat atcaatttga ttcaggagag cggtaccccc
     2101 tgcgtcatgc tcgatcgtgt ggatcctggg cttaacgtca gcgttatcca ggttgatgaa
     2161 caactggcgg cgatgcaggt aacacaacat cttattgatc acggataccg cgacatcggc
     2221 atcatctgcg gcccgcgtga aatgctgaat actcaggatc gtatccgggg ctggcagcag
     2281 gcgctggaag cctcatcgtt agtggtcaat ccctcatgga ttttttcgac caactatacc
     2341 cgcgccggcg gttacgaggc gacaaaacgc atgcttgagc accaactgcc acgcgccctg
     2401 ttcgcgacaa atgaacaaca ggctctcggc tgtttacgcg ctttggcaga acatgggctg
     2461 cgcgttcctg aagatgttgc gctggtctgc tttaacgcaa cacaagaatc ggcttataac
     2521 gtcccttctt taaccgccgt tcgacaacct gtcgataaga tggcccgtgc ggcaattgac
     2581 atgctgaaaa attgggatgg agaagttcgc cgtgttgaat ttgagtttta tttacgaaca
     2641 ggagagtcgt gtggctgcca agggcatgaa gttcaacccg agaaaaagtg aaaatgaatc
     2701 gatgcgtatc attattgact gcgatccggg gaacggcatt cccggcgcta atatcgacga
     2761 cggtctggcg ctggcgctgg ccattgccgc accgcaaatc gcgctggaaa tgatcaccac
     2821 tgttgccgga aatacgccgg tagacgtggg atatgcggtc gctagagatc tcattacaca
     2881 attggatatt cctgttgcgg tctaccgtgg tgcctcgcgt gcgctcttgg aagatccgca
     2941 accctggcgt gaaaaactgg atcatggtgt cgatcagttt ggtttacggc aactctggtc
     3001 gaatgttcct gctccagcat tgtgccagca ggtagaaccc catgcgcctg aagcaatagg
     3061 tgaactcatc tgtcgtaatc caggcgaaat aacgttggtt gctaccggcc ccctcactaa
     3121 tgtagccatc gccctgcagc tttacccgca gattgttcac gcggtcaaaa atatcgtcgt
     3181 tatgggtggc gtgttcaatg ttccaggcta cctgaaagat acaaattttg gtctggaccc
     3241 tgaggcggct catgcggtgc tcaccagcgg tgcgccagtc acgctggtcc cgatggatgt
     3301 gacaacgcaa acccaaatgc ttcacgccga tttggatcgt ctagcaaaaa cagaaaacgg
     3361 gcttagccgt tatttggcac aaaccattcg accatggatt acatactcta tgcaaacccg
     3421 caatctgcct gggtgttgga tccacgatgt gttaaccatt gcctggttac tggatccctc
     3481 tcttgcaaca acggctgaag attatctgga tgtatctctg gaaggcatta cacgcggaat
     3541 gacttgttgc tatggacgtg acacattacg cctcaatatt gggatccctg aaccaaaagg
     3601 tgcacaggtc acaattctgc agagcatcga taacccgcgg cttatttcgc tgatagagca
     3661 ctatatccag aactacggcg cgtagcctca tggggcagaa aaggagacct tcgtacacag
     3721 tttggagtga gttttttccg gaggaaatat gaatacgtta tccttcttat tattcagtga
     3781 agaagaatct ctctttatta cactgggcaa aatttgcgtc gcgtttatcc ttggtggcat
     3841 tattggcctt gaaagagaat cgaaagggaa gcctgttgga tttaaaacgt gcgtcattat
     3901 ttcagtcgcc agttgtgttc tgacaattgt atcgattcag tctgcagaat attacgctga
     3961 aatctcgatg aatatacgta gcgatccgat gcgactggca gcacagatta tcagcggtgt
     4021 aggttttctc ggcgcaggtg ttatccttca tcgccatgac gatgctatat ctggactaac
     4081 aactgcagct atcgtttggg cttcggctgg ggtgggaatt acatgtggtg ctgggtttta
     4141 tatgcatgct ttggttacca ctgctttgtt catgattgca ataaaactca gctattttgt
     4201 gctctttttg caagctaaga atcaatttcc gggtaaagta aaagttcatg ttactcttga
     4261 cgaaagatca gggctacaag ccctgataga gtgtattaat caacaaaaaa atattattga
     4321 agcgttcact attcgcgatg ttaaaaaggg aagaatagaa gttaacctta aggtcgtgat
     4381 tagaaaaaaa atgacattac cagaactata ccattcactc tctaagcttg aacatgtttg
     4441 cgctatcgcg ctggaacact gaaagcatcg attagaggct aaaacagcaa taaaaacatt
     4501 cacaataaca attagtcaag tctggatatc atatgttcac agaggcagaa tgctctgtgt
     4561 taattggtac tgcaggaatt aaataatggt accccctact gtactagctg tctcttgatc
     4621 agagctcctg atcaagagac tccatcacca ggtaaccctc aaccatatcc tgaagtctga
     4681 accagccatc ccacatgact acacaaccgg ggcgacctgt gcgtttgctg tcatgccatc
     4741 gcctcagttt cgccagtttc agacaggccc atttcagtgt cggcgtctgt gacggaagcg
     4801 gttttccttc cagcttaacc cacagcagtt tccactctgt cggcaacagt attttctcac
     4861 agctgtcatt ttgtgtttct tcactgatac ctccctgccg caggcccagc acccgtaccg
     4921 cgataaacgc cttgataacc actatgcgct caaggttatc ccgggtctgc atacgcagcg
     4981 attccacaca tgtaccacca cttttccacg ccttgtggta ttcctctatc aaccagcgtc
     5041 gctcgtaatg gctgacgata cgtcgcgcat cggcggcact cgccactttt tctgatgtca
     5101 gcagatgcca gcaggcaccg tcctctgcct gctcccggca acagacatac gtgagcggaa
     5161 gcgcctggcc gctgttgtcg ggatttttta tgccgacttc gctgtaactg gtgaacatcc
     5221 gggcctggcg ggctgcgcgc ccgccttttt acatcacatt cagcatgtgg cttcatgcgg
     5281 ttgtcaggac ttccggcagt tcgaagagct tgccgggtgc ttcttcctgc cggctattct
     5341 gtgcagcacg caccacgaag cgctgtccgt ggctgacttt ataatgcagg taatgccaga
     5401 tatccgcttc caggccacag acagtgatta cccgtttctg tatctccccc agccgttcag
     5461 ccatacgctc cgaagcctgc tgccagcgat aactttcttt ttcttcacag ggacgttctt
     5521 ttcgctggtg cttaacaccg taggtgtccg tggcccgact ccagcgctgc tgttcgataa
     5581 gaccgactgg cagggcgctg tcgggggcat acatcaggac agagtgagcc agcagcccgc
     5641 gcgtcttcgg gttagtagtg gtattcccta ggtcatcaaa tgccgtactg tggctgaaac
     5701 taatggtggt ggtgtcttcc agtgcgagga gcagcggatg tgcctcacat gcccttgcag
     5761 tggcggtaaa tccggcttcg gcaatggctt ccggagacac tgacgggtta cgcatcaggc
     5821 ggtacgcacc ttcaacctga gcagtggact gggatgattt cacgatagaa agacctgcat
     5881 gctgagcgag agaagaggcc agtgacacaa ggcgtcgtgt acgacgcgga tcaccgagac
     5941 tggcatgtcc aaactgctcg ttagcccatg aataacaatc agaaagtacc ataacagacg
     6001 taagcgttaa cggagcaccg tattgacggt tatttattat aaccttgcgg ctcgcctccg
     6061 ggtacacgaa gaaggtggca acattcggcc agtttgcctg ccagctgcga cttatctgcg
     6121 ggtaccgtgc atcccattcc ttcccgaacg catccagcgc ctgcaacgcg ccttcttccg
     6181 tggctgtgtg gtaaacctgt ttcaggtcac gggttaccgc tttatagtct ttccacgccc
     6241 taaccgcagg ctgttgcgta ccatatgtac tatgcacaac aggagctgcg tctccgggta
     6301 gacggcatta atcgcttccg ggaagccttt caggccgtcc acgcaggcga taaggatatc
     6361 gttcaggccg cgatttttca gttccgtcag tacattcagc cagaactttg caccttcatt
     6421 ttcggccagc cacatacctg gcagctcttt ctgaccatca aggttaatac ccagagccag
     6481 gaacaccgct ttgctgataa cccggctgtc ctgccgtact ttcaggacaa tacagtcaag
     6541 ataaacaatg ggataaatcg catccagagg acggttttgc cattctacaa cctggtcgat
     6601 tacggcatcg gtgattttcg atatcagcgc cggagaaacg tctgcatcgt acatttcttt
     6661 gaagactgag gtaatttcgc gagtagtcat ccctctggca tacagcgcca ggatctggtt
     6721 gttcatgccg ataatgcagg tcttattttt cttcaccagt tgtggttcga aggaaccatc
     6781 acgatcacgt ggagtacgta attccatcgg cccgtcggta gtgggacggt tttcgtggaa
     6841 tagccattac gggagttggt tcccgatttg ggttgatttt tttcatgtcc cagatggtga
     6901 gtcagttcag cgttgagagc agcctcaact ttgattttct tgagcaggcg atcgaactga
     6961 ctgagatctt caggagtttt gagatttttg gccagttcgt tagccagagc ctgcaattgt
     7021 ttgtcggcca taaattcacc ttcatttgaa ggtgaattga acatatcaaa atcaggcaat
     7081 tacacaaatc tatgtacagg ctcctttctg gtccagaata atacggttat ccttcacttc
     7141 tgtcatcctc ttttggtata
//
LOCUS       DAOTRK010000197         7050 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.198, whole genome shotgun sequence.
ACCESSION   DAOTRK010000197 DAOTRK010000000
VERSION     DAOTRK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.198"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(111..806)
                     /gene="aqpZ"
                     /locus_tag="QIE41_004300"
     CDS             complement(111..806)
                     /gene="aqpZ"
                     /locus_tag="QIE41_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDS3533097.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGDYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1232..2890
                     /locus_tag="QIE41_004301"
     CDS             1232..2890
                     /locus_tag="QIE41_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDS3533098.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2887..3843)
                     /locus_tag="QIE41_004302"
     CDS             complement(2887..3843)
                     /locus_tag="QIE41_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDS3533099.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3994..5109
                     /gene="macA"
                     /locus_tag="QIE41_004303"
     CDS             3994..5109
                     /gene="macA"
                     /locus_tag="QIE41_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDS3533100.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVTIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKSGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5106..>7050
                     /gene="macB"
                     /locus_tag="QIE41_004304"
     CDS             5106..>7050
                     /gene="macB"
                     /locus_tag="QIE41_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDS3533101.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1723 a         1719 c         1924 g         1684 t
ORIGIN      
        1 gctccctgcg ccagtgacta tatgtggctt aacacatcaa ggagcgcaac aggaagatga
       61 atcaatatat ttaaaaaagg cctgacattg cgccaggcct tctgcgttaa ttaattacgc
      121 ttttccagta gagtccggta aatcagacca ccgataatac cgccgacaat tggcaccacc
      181 cagaagaacc acagttgttc taacgcccag ccgccctgga agatagcaac cgcggtgctg
      241 cgcgccgggt taacagaagt gttagtcacc ggaatactga ttaagtgaat cagggttaag
      301 gccagaccaa tagcgatcgg cgcaaaacca gcaggagcga atttgtcggt tgcgccgtgg
      361 atcaccagca ggaaacctgc actcaatacc agttcaacta ccagcgcgga aagcatggaa
      421 taatcgcctg gcgaatgctc gccataaccg ttagaagcaa aaccgctggc tgccgcgtca
      481 aaacccgttt taccactggc aattaaatac agcagcgccg ccgcaacaat accgccgaca
      541 acctgggcaa ttacgtagcc aacgacttct tttgccggga aacgtccgcc agcccataaa
      601 ccaatagtga ccgccgggtt aaaatgacca ccagaaatat gaccaacagc aaaggccatc
      661 gtcagaacgg tcagaccgaa tgccagcgcc acgccggcaa aaccaatgcc taattccggg
      721 aagcctgcgg ccagtacggc actaccacag ccaccaaaaa caagccagaa agtaccaaaa
      781 cattcagctg ctaattttct gaacatatcc acctcaatta aaaattgacc ctgtgaaaaa
      841 tatggtcgtt ttatagggtc gtcgtaaaaa agtgacgacg gaaataatgc gcggctattt
      901 taaaaacgaa ggcgagtcat tcaccagata aataaatcca gtaaatttga tttagggcaa
      961 cagcgggttg ccccatatag tcatttgtct gattgacagt gtagtgcaag caaaagattt
     1021 aatcctttag gcgtaataaa aaataattta tcatgctaat tatttgattt tgttgttttt
     1081 gcaggcttat cagcaagagg gagtataacg cgattattcg ctcatttttc agacatttgc
     1141 catgcttaaa tgtgatgtca tcacgtatta gcatggtctt tcccgttata ctgccagcgt
     1201 aaaggataag tcacatattt ctggagggga tatgattctt gagcgcgttg aaattgtggg
     1261 ttttcgcggt atcaaccgtt tgtcgttgat gctggaacaa aacaacgtcc tgattgggga
     1321 gaacgcgtgg ggtaaatcca gcttgctgga cgccttaacc ctgctgctat cgccagaatc
     1381 agatctctac cattttgagc gcgacgattt ctggttcccg ccgggagata tcaacgggcg
     1441 agaacatcat ctgcatatta ttttgacctt ccgcgaatcg ctgccaggcc gacatcgggt
     1501 tcgccgctat cggccgctgg aagcgtgctg gacgccatgc accgatggct atcaccgtat
     1561 tttttatcgt ctggaagggg agagtgcgga agacggcagc gtgatgacac tgcgcagttt
     1621 tctcgataaa gacggacatc cgattgatgt cgaggatatt aacgaccagg cacgccatct
     1681 ggtgcgttta atgccggtgc tgcgcttgcg tgatgcccgt tttatgcgcc gtattcgtaa
     1741 cggcacggtg ccaaatgtcc ctaatgtgga agtcaccgcg cgccagctcg atttcctcgc
     1801 ccgtgagtta tcctcacatc cgcaaaatct ctctgatggg cagattcgtc agggactttc
     1861 cgcaatggtg caactgcttg agcattattt ctctgagcag ggggccggac aggcgcgata
     1921 tcgtttaatg cggcggcggg ccagcaatga gcaacgaagc tggcgctatc tggatatcat
     1981 caaccggatg attgaccgac ctggtgggcg ctcgtatcgg gttattttgc tcggcctgtt
     2041 tgctacttta ttgcaggcaa aaggcacatt gcgactggat aaagacgccc gtccattgtt
     2101 gctgatcgaa gatccagaaa cccgtttaca ccccattatg ctttcagttg cctggcatct
     2161 gttgaatctt ctgccattgc agcgcattgc caccaccaac tcgggtgagt tgctttcgtt
     2221 aacgccggta gagcatgttt gccgactggt acgtgagtcc tcgcgcgttg ccgcctggcg
     2281 tctggggccg agtggcttga gtaccgaaga tagccgacgc atatcctttc acattcgttt
     2341 taaccgtccg tcatcgctgt ttgcacgctg ctggctgctg gtggaagggg aaacggaaac
     2401 ttgggttatc aatgaactgg cgcgtcagtg cggacatcat tttgatgccg aagggatcaa
     2461 ggtcattgag tttgcccagt ccgggctaaa gccactggtt aaatttgccc ggcgaatggg
     2521 gattgaatgg catgtactgg tcgatggcga tgaagcaggg aagaaatatg ccgctacggt
     2581 acgcagcctg ttgaataacg atcgggaagc cgaacgagaa catttaacgg cgttaccggc
     2641 gctggatatg gaacatttta tgtatcgcca gggattttcc gatgtgttcc accgcgtggc
     2701 gcaaatcccg gaaaatgtac cgatgaatct gcgcaaaatt atctcgaaag cgatccatcg
     2761 ctcttccaaa cccgatcttg ccattgaagt ggcaatggag gcaggacgtc gtggtgtgga
     2821 ttccgtaccg acgctgctga aaaaaatgtt ctcacgcgtg ctgtggctgg cgcgcggtcg
     2881 cgcggattaa ccgcgaaaca tcgtggccat ttgtggctga atagcgtcga gcatctcata
     2941 gcgccgacgg tattcagccc gttttttact ggcgatttcg gcaatctctt ttcgtgctat
     3001 ctgtgccgga aggcggtaat ggcgttcagc atcacatacg ccgccaaccg actcccagaa
     3061 agcgttgtaa tcggcatgga tcttgccttc tttatcgcga taacgcaggc tgcggtaaat
     3121 atgcgtttca ttgctgacgg caataatctg ctctacctgc aaacgttggg caaacagaca
     3181 ggctgcttcc atcacaaggc gtttgggaaa tagcccgtgg caggctttcg tcgcattctg
     3241 gatttcctga tgtggaattt cccattttgc gccttgcagt ccgccaataa acattgttct
     3301 tttcccctga tattcacaca gggtaaacgt gatctctgcc agaggaatac cttcgctgtt
     3361 gcggaacagg attgtgctgt caccttcttt atccattgag atcatcatgg tcagctcaag
     3421 tgtgaactgc tcgccgtttt tgccttccag cttcgccagt tgcagcccgg gggtattcaa
     3481 atataagctg aattcttccg ccgacataca cccgcggagt aacgcataat ggtaacgtaa
     3541 cgcctccagc aattgcttac ggctaagatt cgccgcaagg taagggcgat gcagacgcac
     3601 aggcagtcgc ggctggcgcg ttaacaacac attgagatta ggccagtggg aaagttcgtt
     3661 catccactca acgcttaaac gcggcataat caacgagcgc agcaaaaatt tctggcgaaa
     3721 actacggcga tgccagaatt tacccggccg acactgtcca cgtgccagac taagaaaaag
     3781 tgacaggctg ctgagagatt cagatggcgt aaaggtccgt tcagttagct gcgacatatt
     3841 catgaaatca atggttatac atgacgtcga tttcaccatt gcgtatctta accaaacatc
     3901 aatagtgtga ttactaacgt aaattttagg gttttgttga tatttcgttg aagttaatga
     3961 cccggattgg catatggagt attcagaata tttatgaaaa agcggaaaac cgtgaagaag
     4021 cgttacgtta ttgcgctggt gatagtcatt gccgggctga ttacgttatg gagaattctt
     4081 aacgcacccg tgccgactta tcagacgctg attgtgcgcc ccggtgattt acagcaaagc
     4141 gtgctggcga ccggaaagct ggacgcgctg cgtaaggttg atgtgggcgc gcaggtcagc
     4201 ggtcagttga aaacgctgtc ggtgacgatt ggcgataaag taaaaaaaga ccagctttta
     4261 ggggttattg atcctgaaca ggctgaaaac cagattaagg aggtggaagc aacgctgatg
     4321 gagctacgcg cgcagcggca gcaggcggaa gcggagctga aactggcgcg ggtgacgtat
     4381 tcccgtcagc aacgtcttgc acaaacgcaa gcggtttcac agcaggatct tgataccgcc
     4441 gcgacggaga tggctgtgaa acaggcgcaa attggcacca ttgacgcgca aatcaagcgc
     4501 aatcaggcct cactcgatac ggctaaaacc aatctcgatt acacccgcat cgttgccccg
     4561 atggccgggg aagtcacgca aatcaccact ctgcaaggcc agacggtgat tgccgcacaa
     4621 caagcaccga acattctgac gctggcagat atgagcacca tgctggtaaa agcgcaggtt
     4681 tctgaagcgg atgtaatcca cctgaagtcg gggcaaaaag cctggtttac ggtgcttggc
     4741 gatccgctga cgcgctacga ggggcaaatc aaggatgtgc taccgacgcc ggaaaaggtt
     4801 aacgacgcta ttttctatta cgcccgtttt gaagtcccca accccaatgg tttgctgcgg
     4861 ctggatatga ccgcgcaagt gcatattcag ctcaccgatg tgaaaaatgt gctgacgatc
     4921 cctctgtcgg cgttaggcga tccggttggc gataatcgtt ataaagtcaa attgttgcgt
     4981 aatggtgaaa cacgcgagcg tgaagtgacg attggcgcac gtaacgatac cgatgttgag
     5041 attgtcaaag ggctggaagc gggcgatgaa gtggtgattg gtgaggccaa accaggagct
     5101 gcacaatgac gcccttgctc gaattaaagg atattcgtcg cagctatcct gccggtgatg
     5161 agcaggttga ggtgctgaag ggcatcaccc tcgatattta tgctggtgag atggtggcga
     5221 ttgtcggcgc ttcgggttcc ggtaaatcga ccctgatgaa tattctcggc tgcctggata
     5281 aggccaccag cggcacctat cgcgtagccg gtcaggatgt tgccacgctg gacgccgatg
     5341 cgctggcgca actgcgtcgc gaacacttcg gctttatttt ccagcgttac catttgcttt
     5401 cgcatttaac cgccgagcag aacgttgaag tacccgccgt ctatgccggt cttgagcgga
     5461 aacagcggct gcttcgcgcc caggagttac tgcaacgact ggggctggaa gaccgtacag
     5521 agtattaccc ggcacagctt tcgggggggc agcaacagcg cgtcagcatc gcgcgggcat
     5581 tgatgaacgg cggtcaggta attcttgccg atgaaccaac cggcgcactg gacagccatt
     5641 ctggcgaaga ggtgatggcg atcctgcatc agctgcgcga tcgcgggcat acggtgatta
     5701 tcgttaccca cgatccgcag gtcgctgctc aggccgagcg ggtgatcgaa attcgcgacg
     5761 gcgaaattgt gcgcaatcct cccgccgttg aaaaagtgaa tgttgctggc gggacggaac
     5821 ctgttgtcaa cacggcctct ggctggcggc aatttgtcag cggttttaac gaagcgctga
     5881 cgatggcatg gcgggcgctg gcagcgaata aaatgcgtac tttactgacc atgctgggga
     5941 ttattatcgg tattgcgtcg gtggtttcaa ttgtcgtggt gggcgacgcc gccaaacaaa
     6001 tggtgctggc ggatattcgt tctattggta cgaatactat tgatgtctat cccgggaaag
     6061 attttggcga tgacgatccg caatatcagc aggcgctgaa gtacgacgac ttaatcgcca
     6121 tccaaaaaca accgtgggtc gcctcagcca cacctgccgt ctcgcaaaac ctgcgcctgc
     6181 gttataacaa tgttgatgtt gctgccagtg ccaatggcgt gagcggcgat tattttaatg
     6241 tctatggcat gaccttcagt gaagggaaca cctttaatca ggagcagctg aacggtcgtg
     6301 cgcaggtggt ggtgctcgac agtaatactc gccgccagct tttcccccat aaagcagatg
     6361 tggttggcga ggtgattctg gtcggcaata tgcccgccag agtcattggt gtggcggaag
     6421 aaaaacagtc gatgtttggt agcagtaaag tgctgcgtgt ctggctacct tacagcacga
     6481 tgtccgggcg agttatgggc cagtcgtggc tgaactccat tactgtcagg gtgaaagaag
     6541 gatttgacag cgccgaggcg gaacagcaac tcacgcgttt actttcactg cgccacggta
     6601 agaaggattt ctttacctgg aacatggacg gcgtcttgaa aaccgttgaa aagaccacac
     6661 gtactttaca actgtttctg acgctggtgg cggtgatttc gctagtggtg ggcggtattg
     6721 gtgtaatgaa tattatgctg gtgtcagtga ccgagcggac gcgggaaatt ggcattcgca
     6781 tggctgtagg tgcgcgagca agtgatgtgt tgcaacagtt cctgatcgaa gcagtactgg
     6841 tttgcctggt cggtggcgcg ttgggaataa cactgtcact gttaattgct ttcaccttac
     6901 agcttttctt acccggctgg gagattggtt tttcaccgtt ggcgctgctg ctggcgtttc
     6961 tctgctcgac ggtcaccggg attttatttg gctggttacc cgcacgaaat gcggcacgac
     7021 tggatccagt agatgctctg gcacgagagt
//
LOCUS       DAOTRK010000198         7045 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.199, whole genome shotgun sequence.
ACCESSION   DAOTRK010000198 DAOTRK010000000
VERSION     DAOTRK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.199"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            185..1795
                     /gene="yeaV"
                     /locus_tag="QIE41_004305"
     CDS             185..1795
                     /gene="yeaV"
                     /locus_tag="QIE41_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDS3533102.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLKEGDDPDRALRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEAPEKTPVLPAGTVFKGDN"
     gene            1827..2951
                     /gene="yeaW"
                     /locus_tag="QIE41_004306"
     CDS             1827..2951
                     /gene="yeaW"
                     /locus_tag="QIE41_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDS3533103.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSKLANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            3007..3972
                     /gene="yeaX"
                     /locus_tag="QIE41_004307"
     CDS             3007..3972
                     /gene="yeaX"
                     /locus_tag="QIE41_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDS3533104.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFMLVATDGKPLPAFTGGSHVI
                     VQMSDGDNEYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHFHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAAHLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(4026..5141)
                     /gene="rnd"
                     /locus_tag="QIE41_004308"
     CDS             complement(4026..5141)
                     /gene="rnd"
                     /locus_tag="QIE41_004308"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDS3533105.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPISWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(5223..6908)
                     /gene="fadD"
                     /locus_tag="QIE41_004309"
     CDS             complement(5223..6908)
                     /gene="fadD"
                     /locus_tag="QIE41_004309"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDS3533106.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
BASE COUNT         1688 a         1842 c         1747 g         1768 t
ORIGIN      
        1 acctgcgtca tttcaccttt tattgtttcc gtttcgtgtt ttatggcttt ccgtattctt
       61 aattgtttaa tttatgtaac atgtaaataa tcaatttgtt aaacacaaca tgcaactcat
      121 taccctgcaa ataattacaa ctggcaaaag ccagagacgg gcttttatgc ccaggagatg
      181 actcatgatg agcaatgtta agaaaaaaga tgtgccgctg ataagcatca gcctggtggc
      241 cattcttttc atcgcagctg cattaagcct tttcccacaa caatcggccg acgcggccaa
      301 cgcaatatac acttttgtta cgcgtacgtt aggttccgcc gtacaggtat tggttttgct
      361 ggcaatggga ctggtgattt atttagccac cagtaaatac ggcaatattc gtcttggaga
      421 aggaaaaccg gaatacagca cgctctcctg gctgtttatg tttatttgtg ccggtttagg
      481 ttcttctacg ctttattggg gggttgctga atgggcctat tattatcaaa cacctggatt
      541 aaatatcgca ccgcgttcac aacaggcact cgaatttagc gtcccttact ccttcttcca
      601 ctggggcatc agcgcctggg caacttatac gctggcctca ttaatcatgg cttatcactt
      661 tcatgtgcgg aaaaacaaag gtctgagcct ttccggcatt attgccgcga ttaccggcgt
      721 tcgcccgcaa ggcccgtggg gaaaactggt cgatttgatg ttcctgatcg ccactgtcgg
      781 tgcgctgacc atttcccttg ttgttaccgc agcaaccttt actcgtgggc tttccgcgct
      841 gaccggttta cccgataact tcaccgtgca ggcatttgtg atcctgcttt ccggcggcat
      901 tttttgccta agctcgtgga ttggtatcaa caacggtttg caacgtctga gcaaaatggt
      961 tggctggggc gcgttcctgc tgccattact ggtgctgatt gtcggtccaa ccgaatttat
     1021 taccaacagc atcatcaatg ccatcggcct gaccacgcaa aacttcctgc aaatgagctt
     1081 attcaccgat ccgcttggcg atggttcatt tacccgcaac tggaccgttt tctactggct
     1141 gtggtggatt tcatacaccc ctggcgtagc aatgtttgtc acacgcgttt cacgcggtcg
     1201 taagattaaa gaagttatct ggggactgat cctcggcagc accgtcggtt gctggttctt
     1261 cttcggcgta atggaaagct atgccattca ccagtttatc aatggcgtaa tcaacgtccc
     1321 acaggtgctg gaaacactgg gcggcgagac agctgtgcag caagttctga tgtcgttgcc
     1381 agccggtaaa ttgttcctcg ccgcatacct gggcgtgatg attattttcc ttgcctcgca
     1441 tatggatgcg gtggcctaca ccatggcggc gaccagtacg cgaaatctta aggaaggtga
     1501 cgaccctgac cgtgctcttc gcctgttctg gtgcgtggtt atcaccctga ttccactgtc
     1561 tatcttattt actggcgcgt cgctggaaac catgaaaacc actgtggtac ttacagccct
     1621 gcccttcctc gttattttac tggtgaaagt cggtggcttt attcgctggc tgaaacagga
     1681 ttacgccgac attccggctc atcaagttga acattatctc ccgcagacac cggttgaagc
     1741 cccggaaaaa acgccagtgc tccctgcggg aaccgtattc aaaggcgaca actgagcgcc
     1801 atcatcctaa cgataaaggt aaccctatga gcaatctgag ccctgacttt gtactacccg
     1861 aaaatttttg cgctaacccg caagaggcgt ggaccattcc tgcccgtttt tataccgatc
     1921 agaacgcctt tgaacacgaa aaagagaacg tcttcgccaa aagctggatt tgcgtcgctc
     1981 acagcagcaa actggcgaat gccaatgatt atgtgacgcg tgagatcatt ggcgaaagca
     2041 tcgtgctggt acgtggtcgt gataaggttt tgcgcgcgtt ctataacgtt tgcccgcacc
     2101 gtggtcatca gttattgagc ggcgaaggga aagcgaaaaa tgtgattacc tgcccgtatc
     2161 acgcctgggc attcaaactc gatggtaatc tggcccatgc gcgtaactgc gaaaacgtcg
     2221 ccaatttcga tagcgacaaa gcgcaactgg ttccggtgcg tctggaagaa tatgccggat
     2281 tcgtcttcat caacatggac cccaacgcca ccagcgtaga agatcagtta cccggtctgg
     2341 gggcgaaagt gctggaagcc tgcccggaag tccacgatct gaaactggcg gcccgcttta
     2401 ccacccgcac gcctgccaac tggaagaaca ttgtcgataa ctatctcgag tgctatcact
     2461 gtggtccggc gcatccaggt ttctccgact ccgtacaggt tgatcgttac tggcacacca
     2521 tgcacggtaa ctggacgctg cagtacggtt tcgccaaacc gtccgaacag tctttcaaat
     2581 tcgaagaggg cacggatgcg gcattccacg gtttctggct gtggccgtgc acgatgctga
     2641 acgtcacccc gatcaaaggg atgatgacgg tcatttatga attcccggtg gattctgaaa
     2701 ctaccctgca aaactacgat atttacttca ccaatgaaga gttaaccgac gagcaaaaat
     2761 cgctgattga gtggtatcgc gatgtgttcc gtccggaaga tttacgtctg gttgaaagcg
     2821 tacagaaagg gctgaaatcg cgtggctatc gtggccaggg gcgcatcatg gccgacagta
     2881 gcggtagcgg aatttccgaa catggtatcg cccacttcca taatctgctg gcgcaggtgt
     2941 ttaaggacta atgacatcgg cggcggtatt ttccgccgct gggctgattt ttgatggagt
     3001 acagcaatgt cagactatca aatgtttgaa gtacaggtga gccaggttga accgcttacc
     3061 gaacaggtga aacgcttcat gctggtggca accgatggca aaccattacc tgcgtttacc
     3121 ggaggaagtc acgtcattgt gcagatgagc gatggtgata acgagtacag caatgcgtat
     3181 tcactactga gttcgccgca tgacacctct tgttatcaga ttgccgttcg actggaggaa
     3241 aactcgcgcg gcggttcccg ctttttgcat cagcaggtaa aagtgggcga tcggttaacg
     3301 atttcaacgc ctaataacct gtttgcgcta attccctcgg ccagaaagca tctgtttatc
     3361 gcgggcggta ttggtatcac ccctttcctg tcacacctgg cagagttgca atacagcgac
     3421 gtcgactggc agttacatta ctgctcgcga aatccagaaa gttgcgcatt tcgtgatgag
     3481 ctagtccagc atccgcaggc tgagaaagtt cattttcatc attcatcaac cggaacacga
     3541 ctggaattag cgcgattatt ggcggatatc gaacctggca cacatgttta tacctgcggc
     3601 cccgaggcgc taattgaagc tgtaagaagt gaagctgcgc atctggacat cgccgccgat
     3661 acgctgcact ttgagcaatt tgcgattgaa gacaaaaccg gcgatgcatt taccctggtg
     3721 cttgcccgtt ccggtaaaga gtttgtggtg ccggaagaga tgactatttt gcaggttatt
     3781 gaaaataata aagccgcgaa agtggaatgt ttatgtcgtg aaggggtatg cggaacctgc
     3841 gaaacggcaa tactggaagg tgaagctgac catcgggatc agtattttag cgatgaagag
     3901 cgtgccagcc agcaaagtat gttgatctgt tgttcgcgcg cgaagggtaa acgcctggtg
     3961 ttggatttgt agtttgccga agccggatgt ggcgctgagt gcgcccagtc cggcttcgga
     4021 agattttact gcggatattc ctgcaataaa ttgtgtaatg cttccgccat cagctcacca
     4081 cgccagccgg aaatcagctc cggcaaattg ttctgcggtt tcagtttcca gtgccagttc
     4141 agcagttggt tgatttgccg acgcgatgcc agcaattcgg cgctgatctt gtgcgtttcg
     4201 ctcacgtcag taatcagcga cttaatcgct ttaaacgctt tacgataacc cggcatgtcc
     4261 atcaggttaa gcatcggctg cggtaaggcc tcttccggca atgtctgcgc tttttccacc
     4321 agcgccagca gcgttttacc gtgaaagcgg atttcgctac cggataagcc caggctgtcc
     4381 agttcgccta aacttcccgg catataacgc gctaccgacc acaaatgctc ttcgcgcacg
     4441 acaaagttca ccgccagatc gcgctcccgc gccttgcgca gtcgccagtc ggctaacagt
     4501 tgcagacagg ccagttggcg tgtgcgtaac tgccaggcat tggtgatatc acgccaggca
     4561 tcttccggtg caacgacttc ctgacgacgc atttgcatca ggcggcattc atccagcgcc
     4621 gcaggtagcc agccggaggc ctccgtttct accataagct ttgcggtgat cggtaacaga
     4681 taccagacat ccgccgctgc gtattcacac tgacgttcgg tcagcggtct ggccagccag
     4741 tcggtgcgcg attcactctt gtccagcgta acgccggaat actcttccac catggaagcg
     4801 aaaccccatg atatcgggcg tccgcagaag gcagcaagga tttgcgtgtc aatcaggggt
     4861 tgtggtaatt cgccaaagac attgaggaac acttccagat cttcactgcc tgcatggaga
     4921 aattttgtga tggacggatc gcgcaggatc gctttcagcg gtgaccagtc ggtgatcccg
     4981 agaggatcaa ttagcgccag atgctcgcca tcgaaaagtt gaattagacc cagttgcggg
     5041 taataggtgc gcgtacgaac aaattcagta tccagggcta tcgccggaaa ggcacggacg
     5101 gcttcacaca aagaagccag cgcatcgtcc gtggtaatca tttggtaatt caaattgtat
     5161 tctctttagt gggcgtcaaa aaaaacgccg gattaaccgg cgtctgacga ctgacttaac
     5221 gctcaggctt tattgtccac tttgccgcgc gcttcgtcac gtaattctcg tcgcaaaatt
     5281 tttccgacgt tagatttcgg taactcatca cgaaactcca ccaacttcgg tactttgtag
     5341 cccgtgagct gacggcggca aaaagtcacc agtgactctt cggtaagcga tggatctttt
     5401 ttcactacga agattttcac cgcttcacca ctggagccgg aaggtacgcc aacagccgcg
     5461 acttcctgta cgccaggatg ctgcatgacg acatcttcaa tctcgttggg atagacgtta
     5521 aaaccggaaa ccagaatcat gtctttttta cgatcgacaa tgcgcaggaa tccttcttca
     5581 tccatcaccg cgatgtcgcc agtgtgtaac cagccatttt tgatgatctc atcggtagca
     5641 tccggacgct gccagtaacc cagcatcacc tgcggtcctt tgacacaaag ctcacccggt
     5701 tgacccggtg gtacttcgtt gtcgtcgtca tccaccagtt tggcttctgt tgacggtacc
     5761 ggcaaaccga tgctaccact atgataatca atatcatacg ggttaacgct gaccagcggc
     5821 gcacactcgg tcaggccata accttccagc agatactgtc cagtcagttt cacccaacgt
     5881 tctgccacca cttgctgaac aggcatccct ccgcctgcgg aaagatgcag actggagaaa
     5941 tccagctgct ggaactcttt attgttcagc aacgcattga acaaggtgtt aacgccagtg
     6001 atagcggtaa acggatattt cgctaactct tttaccaacc ctggaatatc gcgcggatta
     6061 gtgataagca ggttctgccc acccagttcg ataaacagca ggcagttaat ggtcagggca
     6121 aaaatgtgat acagcggcag cgccgtcacc accagctctt tgcccggatg caacagcgga
     6181 ccataggtcg cgttaacctg ttccaggttc gccagcatat tgcggtgagt cagcatcgcg
     6241 cctttcgcca caccagtggt accgccggtg tattgcagga aagctaaatc ttccggcacc
     6301 agttcgggtt tgacgtactg catccggtag ccgttgtgca gggcgctacg aaatgaaatg
     6361 gcatctggca gatggtattt cggcaccaga cgcttgatgt atttaacaac gaaattgact
     6421 accgtgcctt ttgccgtaga tagctgatcg cccatacggg tcaaaataac gtgctgaacg
     6481 gcggttttat caaccacttt ttccagagtg tgggcaaagt tagacacgat aacaatcgcc
     6541 gatgcgccgc tatcgttaag ctgatgctca agctcacgcg gggtatacaa cgggttaacg
     6601 tttacaacga tcatcccggc acgcaaaatg ccaaacagcg ccaccggata ttgcaataaa
     6661 ttaggcatca tcaacgcaac gcgatcgcct ttcttcagtc ccaacccttg ttgcaaataa
     6721 gcggcaaacg cgcgactgcg ttcttccagt ttgcggaagg tcatcacctc ccccatattc
     6781 acaaacgcgg gttgatcggc gtagcgcgcg accgactgct caaacatatc taccagagat
     6841 tgataacggt cagggttgat ctccgtcgga acgtccgcgg gataacggtt aagccaaacc
     6901 ttcttcaatt cttcacctct aaaatgcgtg ttcgtcgtca tcgcaacccc aaatgatata
     6961 catgccgtta acataatatt aactcatcat accagcttga taattaccca acgaaaaggt
     7021 tgcgaagcgc gtcactattt atttt
//
LOCUS       DAOTRK010000199         7039 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.200, whole genome shotgun sequence.
ACCESSION   DAOTRK010000199 DAOTRK010000000
VERSION     DAOTRK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.200"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            99..299
                     /gene="glgS"
                     /locus_tag="QIE41_004310"
     CDS             99..299
                     /gene="glgS"
                     /locus_tag="QIE41_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDS3533107.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            complement(334..633)
                     /gene="ubiK"
                     /locus_tag="QIE41_004311"
     CDS             complement(334..633)
                     /gene="ubiK"
                     /locus_tag="QIE41_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDS3533108.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     regulatory      755..903
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1007..1660
                     /gene="ribB"
                     /locus_tag="QIE41_004312"
     CDS             1007..1660
                     /gene="ribB"
                     /locus_tag="QIE41_004312"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDS3533109.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            complement(1714..2763)
                     /locus_tag="QIE41_004313"
     CDS             complement(1714..2763)
                     /locus_tag="QIE41_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDS3533110.1"
                     /translation="MITLFRLLAILCLFFNVSAFAVDCYQDGYRGTTLINGDLPTFKI
                     PENAQPGQKIWESGDINITVYCDNAPGWSSNNPSENVYAWIKLPQINSADMLNNPYLT
                     FGVTYNGVDYEGTNEKIDTHACLDKYEQYYNGYYHDPVCNGSTLQKNVTFNAHFRVYV
                     KFKSRPAGDQTVNFGTVNVLQFDGEGGANMAPNAKNLRYAITGLDNISFLDCSVDVRI
                     SPESQIVNFGQIAANSIATFPPKAAFSVSTIKDIASDCTEQFDVATSFFTSDTLYDNT
                     HLEIGNGLLMRITDQKTQEDIEFNQFKLFSTYIPGQSAAMATRDYQAELTQKPGEPLV
                     YGPFQKDLIVKINYH"
     gene            complement(2760..3500)
                     /locus_tag="QIE41_004314"
     CDS             complement(2760..3500)
                     /locus_tag="QIE41_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDS3533111.1"
                     /translation="MKLPLITTGMICFLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRDYISTLPPMERIEPDEQVQIRLMALASLNNLPQD
                     RETLFYYNVREIPPRAKEQNVMQIAMQSRLKLFWRPKAIELKEGEMVPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVAPFASATQHLNNLPSTFQLGFVAD
                     YGGLEMFKVVCNSVQSLCQSTPAKKGKI"
     gene            complement(3516..6038)
                     /locus_tag="QIE41_004315"
     CDS             complement(3516..6038)
                     /locus_tag="QIE41_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDS3533112.1"
                     /translation="MNNKNTFTGDKLSHAIKIALSGVVCSLLFVLPVHAVEFNVDMID
                     VEDRENIDISRFEKKGYITPGKYLVRVQINKNMLPQTLILEWVKADNESGSLLCLTKE
                     NLTSFGLNTEFIESLQTIAGSECLNLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDAGIAGFILDYNMYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNHDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQQQNSDNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYGDNAR
                     MMVDTDGISGVEINSNRTVTNGLGIAVIPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDQATGKDVGLVAEDGFVY
                     LSGIQENSILHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            complement(6125..6688)
                     /locus_tag="QIE41_004316"
     CDS             complement(6125..6688)
                     /locus_tag="QIE41_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDS3533113.1"
                     /translation="MFKKTLLSMFATALLSGVAFNALADDANQGSGKITFKGEVIDAP
                     CSIAPGDEDQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSS
                     ASVDATDSNLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNF
                     KARMESLGRTATPGNVQAQANYVLDYK"
BASE COUNT         1832 a         1509 c         1682 g         2016 t
ORIGIN      
        1 cgcacgttat gtttaaaggc actacactga ttggggaata ctgaaatcag aaaaagcata
       61 tggccttaaa aggtatgcga taaccggagg atgtgcttat ggatcatagt cttaattctt
      121 taaataattt cgatttcctg gcgcgtagtt ttgccagaat gcacgcagaa ggtcgcccgg
      181 tcgatattct ggccgttact ggtaacatgg atgaagaaca tagaacctgg ttttgcgcac
      241 gttatgcctg gtattgccaa cagatgatgc aaaccagaga acttgaatta gaacactgat
      301 attacgggcc gtctggcccg tttttgtgtt gctttattct gctttatcaa taggcgggat
      361 agccggagga gattgaaggt ctgctaccga attattgcgc gcttccagtt cactgatgcg
      421 ctgctccagt aatgccagtt tctcacgagt ccgtaacagg acttgcgtct gtacgtcgaa
      481 ttcttcgcgg ctcaccagat cgagacgcgt cagctgcgct tgtagggttt ggcggatttt
      541 tttctccaca tcttccccga actccctgat tcctttaggc attgattcgt gaacctggcg
      601 agcgatttgc tcaatttttt tcgggtcaat cattgtagtt tccctgtact gataggtgtt
      661 gagtgccatt gtagtgcgat aagggtaagt cataaaccag aattatgtga agctatgcgt
      721 tgctgccgct aatcattagc gttatagtga atccgcttat tctcagggcg gggcgaaatt
      781 ccccaccggc ggtaaatcaa ctcagttgaa agcccgcgag cgctttgggt gcgaactcaa
      841 aggacagcag atccggtgta attccggggc cgacggttag agtccggatg ggagagagta
      901 acgattctgt cgggcatgga cccgctcacg ttattttggc tatatgccgc cactcctaag
      961 actgccctga ttctggtaac cataatttta gtgaggtttt tttaccatga atcagacgct
     1021 actttcctct tttggtacgc ctttcgaacg tgttgaaaat gcactggctg cgctgcgtga
     1081 aggacgcggt gtaatggtgc ttgatgatga agaccgtgaa aacgaaggcg atatgatctt
     1141 cccggcagaa accatgaccg ttgagcagat ggcgctgacc attcgccacg gtagcggtat
     1201 tgtttgcctg tgcattactg aagatcgccg taaacaactc gatctgccaa tgatggtaga
     1261 aaataacacc agcgcctatg gcaccggttt taccgtgacc attgaagcgg ctgaaggtgt
     1321 gactaccggt gtttctgccg ctgaccgtat tacgaccgtt cgcgcagcga ttgccgatgg
     1381 cgcaaaacct tctgatctga atcgcccggg ccacgttttc ccacttcgcg cgcaggcagg
     1441 tggagtactg acgcgtggcg gtcatactga agcaactatt gatctgatga cgctggcagg
     1501 ctttaaaccg gctggtgtgc tgtgtgagct gactaatgac gatggcacga tggcgcgtgc
     1561 accagagtgc attgagtttg ccaataaaca caatatggcg ctcgtgacta ttgaagacct
     1621 ggtggcatac cgtcaggcac atgagcgtaa agccagctaa tgagatgaaa tactaaatat
     1681 gaaaatgccg gggtgttccc ggcattttgc tgtttagtgg tagttgattt taactatcag
     1741 gtctttctga aatgggccat agacgagtgg ttcaccaggt ttttgggtta attcggcctg
     1801 gtaatcgcgg gttgccattg ccgcactctg accgggaata taagtactaa ataatttgaa
     1861 ctggttaaat tcaatatctt cttgcgtttt ttgatcagta attcgcatga gcaagccgtt
     1921 acctatttcc agatgcgtat tgtcatataa tgtatctgaa gtaaagaaac tggttgcaac
     1981 atcaaactgt tcggtacaat cagacgcaat gtcttttatg gtagaaacgc tgaatgctgc
     2041 cttcggtggg aaagttgcaa tggaattcgc agcgatctgc ccaaaattga ctatctgact
     2101 ttccggggaa atgcggacgt cgacactaca gtcaaggaat gaaatattat ctaaccccgt
     2161 aatcgcatag cgtaaatttt tcgcgttggg ggccatgttc gccccgcctt caccgtcgaa
     2221 ttgcagcacg ttgactgtgc caaagtttac cgtctgatct cctgccgggc ggcttttgaa
     2281 ttttacatag acgcgaaaat gggcgttaaa tgttacattt ttttgaagag tgctgccatt
     2341 gcatacaggg tcatgataat acccattata gtattgttca tatttatcca ggcacgcatg
     2401 agtatcaatt ttttcatttg tcccttcata atctacacca ttataagtca cgccaaatgt
     2461 taaatacgga ttattcaaca tatcggcact atttatttgg ggcaatttga tccaggcata
     2521 gacattttct gatgggttat tacttgacca tcctggtgca ttgtcacaat aaactgtgat
     2581 attaatatct ccgctctccc aaattttttg cccaggttgc gcattctctg gaattttgaa
     2641 cgttggtaaa tctccattta tgagggttgt tcctctgtac ccatcctgat agcaatcaac
     2701 agcaaaagct gaaacgttaa aaaaaaggca aagaatcgcc agtagtcgaa aaagtgtaat
     2761 catattttcc ccttcttcgc aggagtactt tggcagaggg actggacgga attacatact
     2821 actttaaaca tctccagccc gccgtagtcc gccacaaagc ctaactggaa cgtagagggc
     2881 aggttgttta agtgttgagt cgcagaagca aatggtgcca ccataatact gtccgcacca
     2941 ggcatcagtg tcttgccatt agtgccgata tagccgacgg taatatgata aggcgtcggg
     3001 ttattcaggg tcagcccggc ggcggtgcgg gtaatcgtca ctttttgcag aggaaccatc
     3061 tctccttctt ttaactcaat cgctttcggt cgccagaata gtttcagacg gctttgcatg
     3121 gcgatttgca tgacattctg ttctttggca cgcggcggaa tttcccgcac gttatagtag
     3181 aatagcgttt ctctgtcctg tggtaagtta tttaacgagg ccagcgccat caggcgaatt
     3241 tgtacctgtt catcaggctc aatacgttcc atcggcggca gtgttgaaat gtaatcacgc
     3301 gtttttttac cgctggcatc ttcaatccac gattgagcaa gataaggatc gattttactt
     3361 tggttggtca ggcgaaccgt tgcgctttta ttcgaagcat taaaaatgat gcgtgtcctg
     3421 tcgggggaga cagtagcctg ggcaaagttg cagatcccca gaaaacaaat cattcctgtt
     3481 gtgattaatg gtaatttcat tatattcatc ctgaattatt tgactgtttt acaaggtaaa
     3541 attatcgcgc tttcactaat gttactttgg tttggcggcg tgacttcaca ggtattatca
     3601 ccccaggtta aatgcagaat actgttttcc tgaataccgc tgagataaac gaagccatct
     3661 tccgctacca ggcccacatc tttacctgtc gcttgatccg tgacattcac acctagtgga
     3721 gggaagcggc cattttcctg acgaataacg ccgacgattt ggtatccggt ggtggcattc
     3781 agttttgcgt agccgatggc accctgggtg agcgtagtac gaataaccga gttttcgaca
     3841 tcgacacctt caggcagatc gttattgttc acccgcaaca tggaggtggt gtagttcgat
     3901 aacgaaggta tcacggcgat gcccagcccg ttggtaacag ttcggttaga gttgatttca
     3961 acgccggaga tgccatcggt atcgaccatc atgcgggcgt tatcgccata gctataatcg
     4021 tgaagggcga caccatgacg tgtagcggta agtgaaccgt accagcctgc ggtaacagaa
     4081 ttgtactgat tcggctgtac actgccatta atgttcagac gtccccacgg cgaatagtgc
     4141 tggtagctgc cgcgcagtgt gggttcgcca tcacgtatat tgtcgtcagt tgccgacgca
     4201 gaaatattcc agatattgtt gcgatctgat gaatcgtacc aggaaatcat ctgcgaagta
     4261 ttactgcttc ccgtacgctg catactgtag gagatgttgc ggttttgttg taaaggtaga
     4321 gagaaagaga agtaatattg attctcttcg tcgtcatccc agcggattcg acttactgcc
     4381 agcgatgcag agataccttt gaaatcgcca atatcaaagt ttttgcttac agatacggag
     4441 tagttggtat tgctggcgct gtcccaataa gtattgcgtg taacgttgag ataagagttt
     4501 aattccagcg tttcaaagta ctggttcaag gaaatgacat aactctcttt ttcattatca
     4561 attgactcat cgccattacg cgagcttagg tactcactca tcgacacgta atctttatct
     4621 gagaagcgat aacccgcgaa ggtaatctga ctacctgtcg attcgaaatg ctttgcatag
     4681 ttgaagcgat agctgtaacc acgcagattg tcgctatttt gttgctgcag agaggcgtct
     4741 gctccggtga catcaaaaga aagcgaaccg aattggttaa ggttaaagcc aataccggta
     4801 gtgatagcct gataatcatc agcggtgaac atgccgccac catacagcga cacattgttc
     4861 agccagcccc aggaggcttc cgccgtccag aaaaagggat tattgatatc gttatgcccg
     4921 acggatgttg gttttcccag cgacgtctta tagcgcactt gccctttacg ggttaaatag
     4981 ggaatggatg cggagccaac ctggaaggtg ctggtgcggc catcttcttc ttccactgtg
     5041 acatccaact gcccctgaaa cgattgcccc aaatcagaaa tagtaaaagg gcctggcgcg
     5101 acagtggttt gataaagtac gcgaccattt tgtgccacag tcactttagc gttggtctgc
     5161 gcgataccgg taatttgtgg cgcataaccc tgtaaatctg gcggcagcat gctttcatca
     5221 ctttccagcg atgcgccagt aaagtggaag gtatcgtaaa gattggagct caggtcgtac
     5281 tggcccatag tgaattttga cgaccaggag gggatagggc gaaacagata agttcgcgca
     5341 aattcgctgt cgtggtttac cgagcgtcca tcagcaaaat tctgattata ttggtaatcg
     5401 ctacgtaagc gccatgcgcc gaggttaaag cctaacgtac cataggagct gacgttttgg
     5461 gtgctgtctc cgtgatgtgg ggcatactgg ctggcataca tgttgtaatc aagaataaac
     5521 ccggcgatac cggcatccca aaactctggt ggcgtccagt ttgttgcctg gtattttaac
     5581 cacgcttggg gaacacttag cgataatatc atcgtagctt tatcaagccg tgtcgttaat
     5641 tcctgacgtt ggcttaaatt gagacattcg ctgccagcta tggtttgcaa tgattcaata
     5701 aattccgtat taagaccgaa actagtcaga ttttctttgg ttaagcagag taacgaacca
     5761 ctttcattat cggcttttac ccattccagt attaacgttt gtggcaacat atttttattt
     5821 atctgcacac gaacgagata tttaccagga gtaatatagc cttttttctc aaaacgcgag
     5881 atatcgatgt tttcacggtc ttcaacgtca atcatatcga cgttgaattc cacggcgtgg
     5941 acaggtaaaa cgaagagtag cgaacacaca acgccagaca gggcaatttt aattgcatgg
     6001 gataacttat ccccggtaaa cgtgttttta ttgttcataa aaatccattt ctaaaaaggc
     6061 ccaaagttgc cgatgaaatt tcatcggctt tatcgcctga cgttgtgtag ttactaattg
     6121 ccgcttactt gtagtcgagt acgtaattag cttgtgcctg cacgttacct ggggtcgcgg
     6181 tgcgtcccag agattccata cgggctttaa agttcaactc ctggtaggag ttagtcgtcg
     6241 ggaagttgat agtgattgga gtgccaagag tcacgttggt gttgtctgat ttcaccagac
     6301 gtacgcctac atcagttgcg ccgccgatgt taccttccag agtgttttta agcaggttgg
     6361 aatcggtagc gtcaacactg gcagaactaa aggtgatttt gactttatca acagtattag
     6421 tgccgtcaga taaaatacaa tcctgcaagt gaatggtgac atctacaggc agagatttct
     6481 gaccgctttt taatacggta tcagcaactt caccgaggtt tattgtctga tcttcatcac
     6541 caggagcaat agaacaaggt gcatcgataa cttcaccttt aaaagtaatt ttaccggacc
     6601 cctgattagc atcgtcagca agagcgttaa aagcaacgcc tgataacaat gcggttgcga
     6661 acatagataa caatgttttt ttaaacatga ctataatcct ttataaatta gaaaggttat
     6721 taatgaggaa aatataggtt tgaatctgat attaattccg ctatggagat taccatagtt
     6781 agagatgact tagcttgtta ttaatattaa atactgatta agtaaaactt aaatcataca
     6841 ctttcattat atattaagat gcgtatacct ggatatttgt tgtatatcgt tgtgaataat
     6901 aatttttttg tatgagttgt ggtgaaaaaa tgaacatcca ttgacaaaag tttacgtatg
     6961 atattgtaag gtggtaaagt atgtttatta aaacgagtag ggacgcaaga atcgtgaaga
     7021 tataatatat atctagtgg
//
LOCUS       DAOTRK010000200         6648 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.201, whole genome shotgun sequence.
ACCESSION   DAOTRK010000200 DAOTRK010000000
VERSION     DAOTRK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.201"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..659
                     /locus_tag="QIE41_004317"
     CDS             <1..659
                     /locus_tag="QIE41_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533114.1"
                     /translation="AESNFNYMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIIN
                     NLYGSEIRIRGINENFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEQNYIHPV
                     GSIFAAIQKNESLLKFPSVISMLNVNLLFNPLNLPGMGSGVLEEIMSISDSSLRKRLG
                     YEVLSFSLQAHSLSQECIDKLAIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA
                     "
     gene            complement(663..2054)
                     /gene="tcyP"
                     /locus_tag="QIE41_004318"
     CDS             complement(663..2054)
                     /gene="tcyP"
                     /locus_tag="QIE41_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDS3533115.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2187..2777)
                     /locus_tag="QIE41_004319"
     CDS             complement(2187..2777)
                     /locus_tag="QIE41_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDS3533116.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2940..3608)
                     /gene="hxpB"
                     /locus_tag="QIE41_004320"
     CDS             complement(2940..3608)
                     /gene="hxpB"
                     /locus_tag="QIE41_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDS3533117.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3755..4291
                     /locus_tag="QIE41_004321"
     CDS             3755..4291
                     /locus_tag="QIE41_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDS3533118.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4332..5192)
                     /locus_tag="QIE41_004322"
     CDS             complement(4332..5192)
                     /locus_tag="QIE41_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDS3533119.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5298..5588)
                     /gene="ghoS"
                     /locus_tag="QIE41_004323"
     CDS             complement(5298..5588)
                     /gene="ghoS"
                     /locus_tag="QIE41_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDS3533120.1"
                     /translation="MVSGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            complement(5689..6618)
                     /gene="pfkB"
                     /locus_tag="QIE41_004324"
     CDS             complement(5689..6618)
                     /gene="pfkB"
                     /locus_tag="QIE41_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDS3533121.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALSEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1751 a         1630 c         1610 g         1657 t
ORIGIN      
        1 tagcagaatc aaacttcaat tatatgattg cctatatgtc taacattaac tatgatgcct
       61 caaagccagg agcgccagca cttagttttg atacatatct gcaggacaat gttaaatata
      121 gagtgataat taataatctt tatggtagtg aaattaggat aagaggtatt aatgagaatt
      181 ttgttgggat ggattttacc tgtgaattcc gaccagaaaa aatgaccaat ttgatcaata
      241 taaaaaacag gttggttatt cactatttaa gaacgatata ctatgaacaa aattatatac
      301 atcctgttgg gagcattttt gctgcgattc aaaaaaatga gagtctccta aagtttcctt
      361 cggtaatatc aatgcttaat gtcaatcttc tttttaatcc actgaatctt ccgggaatgg
      421 gaagtggtgt gcttgaagag attatgagta tttcagatag ttcacttaga aaacgtcttg
      481 ggtatgaggt tttaagcttc tcgctacaag cacattcatt gagtcaggaa tgtatcgata
      541 agttagccat ttttttcgct gacgatctgt ttaaatatga gtcagtatgc atcgccgcga
      601 tggaacattt aaaaagtaag gcaacggccc ctatacaaaa cggaccgttg ccagcataag
      661 aattaacggt gtgccagttc ggcgtcgtct tcgctatcca gaatggcttt atcggtttgc
      721 ttcagccact ggctggtcag cgtgccagcg gtcatcgagc cactaacgtt tagcgccgta
      781 cgccccatgt cgataagcgg ttcaacggag attaacaatg ccaccagcgt tacaggcagg
      841 cccatcgcag gcagcacaat cagtgcggcg aaagttgcac caccaccgac accggcaacg
      901 cctgcggaac taacggtaac aataccgacc agcgtcgcaa tccacatcgg gtccatcggg
      961 ttaatgccaa ccgtaggcgc aaccatcacc gccagcattg ccggatacaa accggcacag
     1021 ccgttctgac caatggttgc accgaaagag gcggcgaaac tggcgatgga ttcaggaaca
     1081 cccagacgac gcgtttgtgc ttccacattc agtgggatag acgcagcact ggaacggctg
     1141 gtaaaggcaa acgtcagaac tggccatacc ttacggaagt acttcagcgg gctcacgcca
     1201 ttaataccca gcagaaggcc atgcactgca aacataatca gaagaccgag gtaggacgcg
     1261 acaacgaaac ttcccagttt gatgatgtct tgcaggttag aacctgcaac cactttggtc
     1321 atcagcgcca gaacgccgta aggggtcagc tgcatgacca ggcgaaccag tttcatcacc
     1381 cagctttgca gggtatcgat agcggttaag acgcgttcac ctttcggcgc atcatccttc
     1441 agcagcttca gcgcagctac gccgaggaat gcggcaaaaa ttaccacgct gataattgac
     1501 gtcggattgg ctccggtcag atcggcaaac gggtttttcg ggataaagga caagaccagc
     1561 tgcggaacgc tcaggtcaga gactttacca acatagttac tttcaatggc gttcagacgt
     1621 gcagtttctg caccaccctg aaccagacct tcagccgtca aaccaaacag gttggtgacc
     1681 agcacaccga ccagcgccgc aatcagcgtg gtaaacaaaa gcgtaccgat ggtcagaaaa
     1741 ctgattttgc ctaactgaga tgcgttatgc agacgggcaa ccgcgctcag aatagaggcg
     1801 aagactaacg gcataacgat catttgcagc agttgaacat agccgttacc aacaatgtta
     1861 aaccactgta cagaatcttt aagtacctgg ctgtcagaac cataaatggt atgcagggcg
     1921 aggccaaaaa ccacacccat caccagaccc accagcactt ttttcgccag actccactgt
     1981 ttatggcggg tctgagccag cgcaaacagc agtacaacga acaccacgat gttcgcaatt
     2041 aatggaaagt tcatccccgt tctcctgatc ttattatttc tatcactgcg tagcagtcat
     2101 agtgttggcg caagattaac agaactgtga gctggctctt atattcaaat ggaattgctt
     2161 attccagaaa ggtgattttt gcctttttag tattcaacct gatgcttaat aagccgatga
     2221 aactggatct gcaaagtatt gatcatttgc gatgaccaac gaacagcgct gttttcgggt
     2281 aatgaatggg gcatccatac cgaccagaca aataatgcca tgcagataaa acgttccagt
     2341 tggttgccct tttggggaac caggataggc aagcggaaac gccagcggca tggccagagc
     2401 aggggaacac cggcgggtgt cagcatatcg gcaagtatgt ggctcaaata acccagcacc
     2461 attccttgta gcgcatcagc cggaatgaac cagctttcag gaaccttaag gtaaaaggtt
     2521 gccagcagcg caaataccgc cagcagactg tgggtaaaac cacgatgccc aaaagcgcgg
     2581 gcgatcggtt ttgatatcca ctttaatcgc tgcccaagaa acgactttgg gtgatcgatg
     2641 tccggtaaca aacacgtcag gattgcggaa gggacaatat gccaccagtc accctgtgcc
     2701 agcacgggcg tcagctcggc atttttggca aataccgcac aagcaataga aaagagaagg
     2761 tgaccttccg ccgtcatgat aaaactcact aaactgtcaa tacgtacagt ataggatttt
     2821 tatacagtgc acggaagtgg tgacggtgta actctttctc ttaaattgac gattaatccg
     2881 tcagttgaat gcaaattcag ataaagaacc aggagcagtg tgatcactgc ccctggtcat
     2941 taaccgagaa ggtcttttgc ggtgagttct gtcagcgatg agagtttgac gtttgctaat
     3001 acaaaacgtg gatcattttg cgcttctggc gcaggaacga cgatggaacg catgcgggct
     3061 gctttagaag cgatcatgcc atttaccgag tcttccagcg ccacgcaggt cagtgggtca
     3121 acgcccagtt ttgctgcgca gtcgagatat acttgcggat gcggcttgct gtaaggcagt
     3181 ttttcagccg aggcgagggc atcgaaactg tcgcgtaagt caaacatggt caacactttt
     3241 tccagcatat gtagtggtga cgcggaggcc agtcccacta ataaaccttg ttctttgcat
     3301 aacgcaacag cttcgcgcac gcctggtaat aatggacgcg tctcttcaac cagtgaaatg
     3361 gcacgagcaa taacccgttc tactacttcc tgacggcttg gcccattcca gggttgccgg
     3421 gcgtaccaaa gatcgaccac catatcgatg cgtaaaccta aggtgtccgg cagctcgtta
     3481 cgacgggaga tatccacccc caggcttgcc atcacatcca gttcagctcg atcccataaa
     3541 ggttctgagt cgataagtaa tccatccata tcaaaaattg cagcaagaat ctgacgcggg
     3601 gttgacatta caacctctcc tttagagcat tatgtgtaaa gtattaagcc gtgcttatga
     3661 ttaagcgcct atcatatcgc ctttaaagaa tgggcgaaaa cagatatcta caggtaaact
     3721 aagtgaaacg acgcgtctta atcaaggaga actcatgacg tatcaacaag ctggacgcat
     3781 tgctgttttg aaaaggattt tgggttgggt gatttttata cctgcattga tctctacact
     3841 gatttctttg ctgaaattta tgaatacccg gcaggaaaac caggaaggca ttaatgcggt
     3901 catgctcgac tttactcatg tcatgatcga tatgatgcag gcgaatacgc cttttttgaa
     3961 tctgttctgg tataactccc cgacgcccaa ttttaatggt ggcgtgaacg ttatgttctg
     4021 ggtgattttc atcctgattt ttgtcggact ggcgttgcag gattccggtg cccggatgag
     4081 tcgccaggcg cgcttcttgc gggaaggtgt tgaagatcaa ctcattctgg aaaaagccaa
     4141 aggggaagag gggctaacgc gtgaacaaat tgagtcacgt attgttgtgc cgcaccatac
     4201 tattttcctg cagtttttct cgctgtatat cctgccggtt atttgtattg ctgcaggtta
     4261 tgtgttcttt tctctgctgg ggtttattta atcaaaaggc cgcacggagc ggccattatc
     4321 ctcaccccat attatgctgc taataatcta tccagtgact gctgagcaat aaccaaatgc
     4381 tgaccgccaa ataaccttgc acgatttaac agcgtgtaga gttggtaaac cggttgacgt
     4441 tcaaggaaat cggcaggtag cggggatact gactgatagc cgtcatagat ttgtggcggc
     4501 tgttcagtat gcagcggtaa catcgccagg tcgcactctc ggtcacccca gtagcaggcc
     4561 gggtcgaaaa tgtacgggcc atccggaccc agcgcacagt tgccggacca taaatcgcca
     4621 tgcaacagag aaggctgcgg ttgatgcgag gccagacgct gctgaatatg ctctacgatg
     4681 gcgtcgatat tgccgaaagc gatccctttc tctgctgcta gttccaactg ccagccaatc
     4741 cgttgttcag caaaaaacgt tgaccagcga cgttgccagg tgttgggctg cggagttgtg
     4801 gagagcgagt tatcaaaatc gaggccaaat tgtggttggt cgctccattg atgtaaacgc
     4861 gcaatttgct gaccaagaat aaatgcgcta tgcgcatcca gcgggcgagg tgggagataa
     4921 tccatcacca gaaaactgta gtcacggtca gcgccaactg cccaaacctt aggcacggtg
     4981 acggttttac tacgcgacag taactccagt tggtcggctt cggcggtaaa accgggaagc
     5041 agttcccttt catcacattt gacgaaaaag tcacgtcctg catagcgcaa atgccatgcg
     5101 gcatggactt ctccgccagg cagttcatta cgcagttcga tttcgccttc acctaactgc
     5161 tcgctcaaaa gacgactgat tgcctgccac atgatgtctc tcccatgttg tctgccagat
     5221 cataaggtta gcgcaagaat gcggtgtaaa aacacgagct aacgcacacc acagactgat
     5281 tattgcccaa aaaccattta ttttctgttg ctattccatt cctcccaggt cgtgatggcg
     5341 atttcaggat ctttaccggt tgcactttgg gtaagccccg aaaccagttc tctaatttct
     5401 tcttcacttt gggtactgat aagtccaaaa gtgttagtcc ccagctcatg gatatttcca
     5461 tcatcatccg tcatggtgag caaaaaaccg tcgcgagtca ggtaattatt caactcgtta
     5521 atttcagtca atgtatcctc atgcaacatg acggttatga cataacggac aagatcgcca
     5581 ctgaccatag ttcacccctt tgttatcaca tgacttttga gcatagtcgg agaaacgcgt
     5641 tgccgacagg ttggtggtga ttcccccaat gcagggggaa tgtttcagtt agcgggaaag
     5701 gtaagcgtaa attttttgtg tatcgtcatg ggagcacagg cgtgttccct gattgagtgt
     5761 ggctgcactc cccgcagcta cgccaaaacg gaccatctct tcaagagagg cattttctgc
     5821 cagtttcagt gtcatcgcgc cgaccatgct gtcaccagcg ccaacggtgc tctggctttt
     5881 caccggtggt ggcacaacct gaatgcagtt ttcactatca acgcccagcg ccccttgtgg
     5941 acccagggaa acgacaactc gtttggcctt gccgctatta acgatttcct gcgcggcttt
     6001 gcggacatcg tctggctggg tgagttcgcg attcactagc gcactgagtt ctttttggtt
     6061 aggcttaacc aactcgatat taccaatcgc cagtgctgca cttaacgctt cgccagaact
     6121 gtcgacgatg cagcgaatcc cttgtttttg cgcagcggaa atcagttggg ttaatttttc
     6181 cagcttcaca cctggcggca ggcttccgct tatgaccagg atagcaccgg attcaatttc
     6241 cagaacttgc tcttcaagct ggcgaaactc atcttcactt aatgccgcgc ctggcatgac
     6301 aaaacgatac tgctcaccgc ttgcttccac atgtacgtgt aaattctgcc gggtccagtc
     6361 tttggcttct acagtagcga cggggacatt ttcatctgcc aacagtgaaa ccagatgttc
     6421 gccggtcgca ccacccgccg ggaagatcgc tgtggcactg cctccaagat gggcaatggc
     6481 gcgggcgacg ttgatgccgc cgcctccggg ttcgaatacc ggtgcggtac agcgcagctt
     6541 tccttcggga taaatttgcg gggtaattgt tgcgctatcg agagagggcg caagtgtcaa
     6601 cgtatagata cgtaccatca tttcctccta taggctgatt tcagtctg
//
LOCUS       DAOTRK010000201         6641 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.202, whole genome shotgun sequence.
ACCESSION   DAOTRK010000201 DAOTRK010000000
VERSION     DAOTRK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.202"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>6641
                     /locus_tag="QIE41_004325"
     CDS             <1..>6641
                     /locus_tag="QIE41_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDS3533122.1"
                     /translation="NGQTVSVLADNGATTAPTVTTQPDGTVEISVTSQTAGVSTVTAS
                     INSSSLIRNVTFVADVRTAQIASLEVTRDNSVADGAMANTLRVKVTDAFGNALNGQTV
                     SVMADNGATVAPTVITEPDGTVEISVTSQTAGVSAVTATINSSSQSQNVTFIADVSTA
                     KIADLVVIKDGSEADGSTANTLQVKVTDAFGNALAGQTVSVMAGNGATTAPTVTTQPD
                     GTVEISVTSQTAGASTVTASINNSSLSQNVTFVADVSTAKIADLVVIKDGSEADGSTA
                     NTLRARVTDAFGNALAGQTVSVMAGNGATVAPTVITEPDGTVEISVTSQTAGISAVTA
                     SINSSSQSRDVTFIADVRTAKIAELEVIRDNAVADGSTANTLQVKVTDANGNTLAGQA
                     VSVLAGNSATVASTVTTKPDGTVEISVTSQTAGTSTVTASINSSSLSRNVTFVADVST
                     AKIADLVVIQDNSVADGAMANTLRMRVTDAFGNTLGGQTVSVTADNSAMVASTVITGP
                     DGTVEISVTSQTAGISIVTASINNSSLSRDVTFVADVRTAKIADLVVIKDGSEADGST
                     ANTLQVRVTDAFGNALAGQTVSVLADNGATVAPTVTTQPDGTVEISVTSQTAGVSAVT
                     ATINSSTQSQNVTFIADVRTAKIADLVVIKDGSEADGSTANTLRARVTDAFGNALAGQ
                     AVSVMAGNSATVTPTVTTQSDGTVEFSVTSQTAGTSTVTASINSSSLSRDVTFIADVR
                     TAQIAVLEVTQDYAVADGSTANTLRARVTDAFGNALAGQTVSVLGGNGATVSPTVITG
                     PDGTVEISVTSQTAGASTVTASINSSSLSRNVTFVADVRTAQIAVLEVTQDYAVADGS
                     TANTLRARVTDAFGNALAGQTVSVTAGNGATVSPTVITGPDGTVEISVTSQTAGVSAV
                     TATINNSTASQNVMFIADVRTAKIADLVVTRDNSVADGAMANTLQVKVTDANGNTLAG
                     QTVSVLADNSATTAPTVITEPDGTVEISVTSQTAGTSTVTATINSSSQSQNVTFIADI
                     RTAQIADLVVIKDGSVADGSTANMLRVRVTDAFGNALGGQTVSVLADNGVTTAPTVIT
                     EPDGTVEISVTSQTAGVSAVTATINSSSQSQNVTFIADVSTAKIADLVVIKDGSEADG
                     STANTLRVRVTDAFGNTLAGQTVSVLADNGATTAPTVITEPDGTVEISVTSQTAGVSA
                     VTASINSSSQSRNVTFVADVRTAQIADLVVIKDGSEADGATANTLRARVTDAFGNALA
                     GQTVSVLADNGATVAPTVTTQPDGTVEISVTSQTAGISAVTASINNSSLSRNVTFIAD
                     VSTAKIADLVVIKDGSEADGSTANTLQVKVTDANGNTLAGQTVSVLAGNSATVTPTVT
                     TKPDGTVEISVTSQTAGISAVTASINSSSQSRNVTFIADVRTAKIADLVVIKDDSVAD
                     GAMANMLRARVTDAFGNALAGQTVSVLAGNSATVAPTMTTKPDGTVEISVTSQTAGTS
                     TVTASINNSSQSQNVTFVPGDASQLTSIVETNKSNYTVGETITITVTLRDAFDNLVTG
                     AASQLAADGVLTVAGTDPSEMGSWVESGGVYTTTRMATIASTNQHANLQLQTWSDGVT
                     SDRYDIQSGSPAQATSTIATDKNAYTAGDTITVAVTLKDAHGNLVEGGESLLSGDNVI
                     VEGAVRSGGWSENAGVYTATWSAQMAGDSHHATLKLSEWGSSKQSESYSIHSGAPVQA
                     NSAIRTDKSAYIAGEPLTVTITLRDEFGNPALGLTSEVIESYIDSFAVGGATPDSMRW
                     VEQNNGEYTIVWTAWVADENLVASLKLKTWATEIKSSLYGIQPGAAAKTQSTIVADKT
                     IYIAGDSITVTVVLKDAQGNFITDGVVQLNEENVQVRNADPIQGNNWVYNGNGQYQRQ
                     YMAHFAEANLNAQLKMAGWSDANYSNNYTIKPGEVSPLGSQLRIREVLVVEGADLPVS
                     ALLVDDFGNPVDNGLDLLDDAVYLQNVEKKEGEKWRYVGDGIYERTYMAYQEGENLTS
                     FMEIKGWRIYGQPSYTILPFVEVELLSVNGVKFRATDGFPETGFDGAKFTLLLTHNMK
                     NTDYNWTAGIYGINVDSNGEVTLSVLIRSEVTITGKPKNGKGNDVVFKFKIKKWFTSL
                     GATSSNTWDIINTSCSYGQMPSSLELAQRPSGGVVPRKVGTLWGEYGNLKTYGNAFSG
                     TDYWTSTQLMGVHEKFNPETGIS"
BASE COUNT         1673 a         1604 c         2107 g         1257 t
ORIGIN      
        1 aatgggcaga cggtcagcgt gttggcagac aacggtgcca cgactgctcc gacggtcacc
       61 acgcagccgg acggcacggt ggaaatcagt gtcaccagcc agacggcggg agtcagtact
      121 gtcactgcca gcattaacag cagcagcctg atccggaacg taacgtttgt cgcggatgtc
      181 aggacggcgc agatagcgag tctggaggtt acccgggata actcagtggc tgacggcgca
      241 atggcgaaca cgctgcgggt gaaggtcacc gatgcgttcg ggaatgcgct gaatggacag
      301 acggtcagcg tgatggcgga caacggcgca acggttgccc cgacggtgat caccgagccg
      361 gacggcacgg tggaaatcag tgtcaccagc cagacggcgg gagtcagtgc agtcactgca
      421 accatcaaca gcagtagtca gagtcagaac gtgacattta tcgccgatgt cagcacggcg
      481 aagatcgctg atctggtggt cattaaggat ggttcagagg cagacggttc gacggcgaac
      541 acgctgcagg tgaaggtgac cgatgcgttc gggaatgcac ttgccggaca gacggtcagc
      601 gtgatggcag gcaacggtgc cacgactgct ccgacggtca ccacgcagcc ggacggcacg
      661 gtggagatca gtgtcaccag tcagaccgcc ggagccagta cagtcaccgc cagcattaac
      721 aacagcagcc tgagccagaa cgtgacgttt gtcgccgatg tcagcacggc gaagatcgct
      781 gatctggtgg tcattaagga tggttcagag gcagacggtt cgacggcgaa cacgctgcgg
      841 gcgagggtaa ccgatgcatt cggtaatgca cttgccggac agacggtcag cgtgatggca
      901 ggcaacggcg caacggttgc cccgacggtg atcaccgagc cggacggcac ggtggaaatc
      961 agtgtcacca gtcagacggc gggaatcagt gcagtcacag ccagcatcaa cagcagcagt
     1021 cagagccggg acgtgacatt tatcgccgat gtcaggacgg cgaagatcgc tgaactggag
     1081 gtcatccggg ataacgcggt ggcagacggt tcgacggcga atacgctgca ggtgaaggtc
     1141 accgatgcta acggtaatac gcttgccgga caggcggtca gcgtgttggc aggcaacagc
     1201 gcaacggttg cttcgacggt gaccacaaag ccggatggca cggtggagat cagtgtcacc
     1261 agtcagacgg caggcaccag tacagtcact gccagcatca acagcagcag cctgagccgg
     1321 aacgtgacgt ttgtcgccga tgtcagcacg gcaaaaatag cggatctggt cgttatccag
     1381 gataactcag tggctgacgg cgcaatggcg aacacgctgc ggatgagggt cacggatgca
     1441 ttcggtaata cgcttggcgg acagacggtc agcgtgacgg cagacaacag tgcaatggtc
     1501 gcttcgactg tcatcacagg gccggacggt acggtggaga tcagtgtcac cagccagacg
     1561 gcggggatca gtattgtcac tgcatccatc aacaacagca gcctgagccg ggacgtgacg
     1621 tttgttgccg atgtcaggac ggcgaagatc gctgatctgg tggtcattaa ggatggttca
     1681 gaggcggacg gttcgacggc gaacacgctg caggtgaggg tcaccgatgc gttcggtaat
     1741 gcgcttgccg ggcagacggt cagcgtgttg gcggacaacg gcgcaacggt cgccccgacg
     1801 gtcaccacgc agccggacgg tacggtggag atctctgtta ccagccagac ggcgggagtc
     1861 agtgcagtca ctgcaaccat caacagcagt actcagagtc agaacgtgac atttatcgcc
     1921 gatgtcagga cggcgaagat cgcggatctg gtggtcatta aggatggttc agaggcggac
     1981 ggttcgacgg cgaacacgct gcgggcgagg gtgaccgatg cgttcggcaa tgcacttgcc
     2041 gggcaggcgg tcagcgtgat ggcaggcaac agcgcaacgg tcactccgac ggtcaccacg
     2101 cagtcggacg gtacggtgga gttcagtgtc accagccaga cggcaggaac cagtacagtc
     2161 accgccagta tcaacagcag cagcctgagc cgggacgtga cgtttattgc ggatgtcagg
     2221 acggcgcaga tcgcggtgct ggaagtgacg caggattacg ccgtggcaga tggttcgacg
     2281 gcgaacacgc tgcgggcgag ggtgaccgat gcgttcggta atgcgcttgc cgggcagacg
     2341 gtcagcgtgt tgggaggcaa cggcgcaacg gtgtctccga cggtcatcac agggccggac
     2401 ggcacggtgg agatcagtgt caccagtcag acggcgggag ccagtactgt cactgccagc
     2461 atcaacagca gcagcctgag ccggaacgtg acgtttgtcg cggatgtcag gacggcgcag
     2521 atcgcggtgc tggaagtgac gcaggattac gccgtggcag atggttcgac ggcgaacacg
     2581 ctgcgggcga gggtgaccga tgcgttcggg aatgcacttg ccgggcagac ggtcagcgtg
     2641 acggcaggca acggcgcaac ggtgtctccg acggtcatca cagggccgga cggcacggtg
     2701 gaaatcagtg tcaccagcca gacggcggga gtcagtgcag tcactgcaac catcaacaac
     2761 agcaccgcga gccagaacgt gatgtttatc gccgatgtca ggacggcgaa gatcgcggat
     2821 ctggtggtca cccgggataa ctcagtggct gacggcgcaa tggcgaacac gctgcaagtg
     2881 aaggttaccg atgctaacgg taatacgctt gccgggcaga cggtcagcgt gttggcggac
     2941 aacagcgcaa cgactgcccc gacggtaatc acagagccgg acgggacggt ggagatctca
     3001 gtcaccagcc agaccgccgg aaccagtact gtcactgcaa ccatcaacag cagtagtcag
     3061 agtcagaacg tgacgtttat cgccgatatc aggacggcgc agatcgctga tctggtggtc
     3121 attaaggacg gctcagtggc agacggttcg acggcgaaca tgctgcgggt gagggtgacg
     3181 gatgcgttcg gtaatgcgct tggcggccag acggtcagcg tgttggcgga caacggcgta
     3241 acgactgccc caacggtgat caccgagccg gacggcacgg tggaaatcag tgtcaccagc
     3301 cagacggcgg gagtcagtgc agtcactgca accatcaaca gcagtagtca gagtcagaac
     3361 gtgacattta tcgccgatgt cagcacggcg aagatcgctg atctggtggt tattaaggat
     3421 ggttcagagg cggacggttc gacggcgaac acgctgcggg tgagggtgac cgatgcgttc
     3481 gggaacacgc tagccgggca gacggtcagc gtgttggcgg acaacggcgc aacgactgcc
     3541 ccgacggtca tcacagagcc ggacggtacg gtggagatct cagtcaccag ccagacggcg
     3601 ggagtcagtg cagtcactgc cagcatcaac agcagcagcc agagccggaa cgtgacgttt
     3661 gtcgccgatg tcaggacggc gcagatcgcg gatctggtgg ttattaagga cggttcagag
     3721 gcggacggtg cgacggcgaa cacgctgcgg gcgagggtga ccgatgcatt cggtaatgcg
     3781 cttgccgggc agacggtcag cgtgttggcg gacaacggcg caacggtcgc cccgacggtc
     3841 accacgcagc cggacggtac agtggagatc tctgttacca gccagacggc ggggatcagt
     3901 gcggtcacag ccagcatcaa caacagcagt ctgagccgga acgtgacatt tatcgccgat
     3961 gtcagcacgg cgaagatcgc tgatctggtg gtcattaagg atggttcaga ggcagacggt
     4021 tcgacggcga atacgctgca ggtgaaggtc accgatgcta acggtaatac gcttgccgga
     4081 cagacggtca gcgtgttggc aggcaacagc gcaacggtca ctccgacggt gaccaccaag
     4141 ccggacggca cggtggagat atctgtaacc agccagacgg cgggaatcag tgcagtcact
     4201 gccagcatca acagcagcag tcagagccgg aacgtgacgt ttatcgccga tgtcaggaca
     4261 gcgaagatcg cggacctggt ggtcattaag gacgactcag tggcagacgg tgcaatggcg
     4321 aatatgctgc gggcgagagt caccgatgcg ttcggtaatg cgcttgccgg gcagacggtc
     4381 agcgtgttgg cgggcaacag cgcaacggtt gctccgacga tgaccacaaa gccggatggc
     4441 acggtggaga tcagtgtcac cagccagacg gcgggcacca gtacagtcac cgccagcatc
     4501 aacaacagca gccagagcca gaacgtcacc tttgtacctg gtgatgcatc gcaactcact
     4561 tcaatcgttg aaacaaataa gtcgaactat acggttggag agacaatcac catcacggta
     4621 acgctccggg atgcgttcga taatctggta accggtgcag cttcacagtt agccgctgat
     4681 ggtgtgctga cggtggctgg cactgacccg tcagaaatgg gaagctgggt tgaatcaggt
     4741 ggcgtgtata ccacaacccg aatggcgacg attgccagta ccaatcagca cgccaacctg
     4801 caattgcaga cgtggagtga tggcgtaacg tcagatcgct atgacatcca gtccggttct
     4861 ccggcgcaag ccacatcaac tatcgctacg gataaaaacg cctacaccgc tggcgacact
     4921 ataaccgtcg ccgtgacgct gaaagacgcg cacggtaacc tggttgaagg gggcgagtcc
     4981 ttactgtctg gtgataacgt aatagtggaa ggagccgtac gttcaggagg atggtctgaa
     5041 aacgccggtg tttatactgc tacatggtcg gcgcaaatgg cgggagactc tcaccacgcg
     5101 acgctgaagt tgtctgaatg gggcagcagt aaacaatcag agagttattc catccacagc
     5161 ggtgctccgg tgcaggcaaa ttctgctatc aggacagata aatcggcata tatcgccggg
     5221 gagccattaa ccgtcacaat aacgttgcgg gatgagtttg gtaaccctgc tttagggctg
     5281 acatcagaag tcatcgagtc ttatattgat agtttcgcgg taggcggcgc aactcccgat
     5341 tctatgcggt gggtcgagca gaataatggt gaatatacca ttgtctggac agcatgggtt
     5401 gccgacgaaa atctggttgc cagtctgaaa ttaaaaacat gggcaacgga gattaagtcc
     5461 tcgctatacg ggatacaacc aggtgcagcg gcaaaaactc aatcaacgat tgtcgcggac
     5521 aaaacgatat atatcgctgg ggatagcata acggtgaccg ttgttcttaa agatgcccag
     5581 ggtaacttta ttactgatgg tgttgttcag cttaatgagg aaaacgtaca agtcaggaat
     5641 gcggatccta tccagggcaa taattgggtt tacaacggca atgggcaata tcaaagacag
     5701 tatatggcgc attttgcgga agcgaatttg aatgcgcaac tgaagatggc tggttggagc
     5761 gatgccaatt attcaaacaa ttacactatt aaaccaggtg aagtgagtcc gcttggttca
     5821 cagcttcgta tacgagaagt tttagtagtg gaaggggcgg atttacctgt cagtgcttta
     5881 ttagttgatg attttggaaa tccggtggat aacggtctgg atttgctgga tgatgcagtg
     5941 tacttacaaa atgtagaaaa aaaagaaggg gaaaaatgga gatatgtggg tgatggaata
     6001 tatgaacgta catatatggc ctaccaagaa ggagaaaatt taacttcatt tatggagatt
     6061 aaaggttggc gtatatacgg acaaccatcc tataccatcc ttccttttgt ggaagtcgag
     6121 ttactgagtg ttaatggtgt aaaattcagg gcgacagatg gttttccaga aacagggttt
     6181 gatggtgcaa aattcacgct gttactaacc cataatatga aaaatacgga ttataattgg
     6241 acggctggga tttatggaat taatgttgac agtaatggag aagtaacatt atctgtgctg
     6301 ataagaagtg aagttactat tactgggaaa cccaagaatg gtaaagggaa tgatgttgta
     6361 tttaaattca agattaaaaa atggtttact agtttaggtg ctaccagtag taatacctgg
     6421 gatatcataa atacttcatg tagttatggt caaatgccgt catctttaga attagcgcag
     6481 cggccaagcg gtggggtagt accgcgaaaa gtaggtacgt tgtggggcga atatggtaat
     6541 ttaaaaactt atgggaacgc ttttagcggt acggattatt ggacaagcac tcagctcatg
     6601 ggagtacacg agaaatttaa tcctgaaact ggtatatcag a
//
LOCUS       DAOTRK010000202         6498 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.203, whole genome shotgun sequence.
ACCESSION   DAOTRK010000202 DAOTRK010000000
VERSION     DAOTRK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.203"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..236
                     /locus_tag="QIE41_004326"
     CDS             <1..236
                     /locus_tag="QIE41_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533123.1"
                     /translation="INVNETRLQASAPAMPAIEALNAFIDKGDYLKSLYVNKTRTAAE
                     QKRLNEILRAQVISGLKASEGLAKLLIASGIML"
     gene            247..555
                     /locus_tag="QIE41_004327"
     CDS             247..555
                     /locus_tag="QIE41_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533124.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFIHILTVMNNLT
                     INYGTETSPELAGINYDDANALITELILFIEDVDAENHEAYPGHLNEITRILNFAIAA
                     "
     gene            complement(607..1197)
                     /gene="cobO"
                     /locus_tag="QIE41_004328"
     CDS             complement(607..1197)
                     /gene="cobO"
                     /locus_tag="QIE41_004328"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDS3533125.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVKALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1194..1952)
                     /locus_tag="QIE41_004329"
     CDS             complement(1194..1952)
                     /locus_tag="QIE41_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDS3533126.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2172..3221
                     /gene="sohB"
                     /locus_tag="QIE41_004330"
     CDS             2172..3221
                     /gene="sohB"
                     /locus_tag="QIE41_004330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDS3533127.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNISEQYKEMKEELAAALMDTHQQKQWHKTQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3257..3508)
                     /locus_tag="QIE41_004331"
     CDS             complement(3257..3508)
                     /locus_tag="QIE41_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDS3533128.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3888..6485
                     /gene="topA"
                     /locus_tag="QIE41_004332"
     CDS             3888..6485
                     /gene="topA"
                     /locus_tag="QIE41_004332"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDS3533129.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1709 a         1511 c         1706 g         1572 t
ORIGIN      
        1 taatcaatgt gaatgaaacc agactacagg catcggcacc tgccatgcct gcgattgagg
       61 ctttaaatgc ctttatagat aagggtgatt atctaaaaag cttatacgtt aataaaacac
      121 gcacggcagc tgaacaaaaa agattaaatg aaatattacg cgcacaggtc attagtgggt
      181 taaaggcgtc tgaggggctg gcaaaattac ttattgccag cggaataatg ttataggagg
      241 ctttttatga acgatgtgca aaaccgtaga atactggcat tacttgatat gctgtataat
      301 actttgggta ataaaaccac atgtaatgtt tgtcataaat attttatcca tatcctcaca
      361 gttatgaaca acctgacgat taactatggt actgaaactt cgccagagtt agccgggata
      421 aattatgacg atgctaatgc gttaattact gaactgatat tatttattga agatgttgac
      481 gctgagaatc atgaagcata tccgggacat ttgaatgaga ttacacgtat tctgaatttc
      541 gctattgccg cttgaatata atcatgatta aagcacctca agaggcttct tttgaggtgc
      601 tttcacttag tagtcaatcc ctatctgcgc ttttacaccg gcatcaaacg catgtttcac
      661 ggggcgtaat tcacttaccg tatccgccag ttcaagtata tcccgatgac aaccacgacc
      721 cgtaataatg acggtttgct gatgtggacg ttcatttaac gccttcacca cttcttcaag
      781 tggcaaataa tcatacgcca ccatataagt cagctcatcg agcaaaacca tatccagtga
      841 aggatcggcc agcattcgtt tggcatgttg ccagacttca cggcaggccg cggtatcaga
      901 ttcgcgattt tgtgtatccc aggtaaagcc cgttgccatc acctgaaact caacgccatg
      961 tggctccagt agattgcgct cgccattagg ccaggtgcct ttaataaact gcacgatacc
     1021 cacttttttg ccatggccta ctgcacgagt tgccgtacca aatgccgcgg tggttttgcc
     1081 ttttccattg ccggtaaaaa cgatgataat accgcgttca tcctgggcct gggccacacg
     1141 agcatcgact ttttctttca ctcgctgctg acgctgttgg tagcgttcat cactcattgg
     1201 gaaattcctg gtttacggcc cggttgggcg tcaaaggtca tgccggtttt acggcggctg
     1261 tcatcgccca tcagccagag gtagagcggc atgatatcag cgggtgtttt aagtttctgt
     1321 ggatcttcgg tcgggaaggc gctggcccgc attgcggtgc gcgtaccgcc tgggttaatg
     1381 cagttgacgc gcaggcgctg ctgatattca tcggccagta cctgcatcat cccttcggtg
     1441 gcaaatttcg acgctgcata tgcgccccag ttggctcgtc cctgacgtcc aacgcttgat
     1501 gaagtaaaga ccagtgaacc ggcgtccgat ttgagtaata aaggaagcag tgcctgggtg
     1561 agcataaagg tggcattaac gttgacctgc atgacgtcct gccagacctg cggattttgt
     1621 tcgctcattg ggcaaacatc gccgagcaat ccggcattat gcaaaacacc atccagacgc
     1681 ggataattaa cggcaatgcg ctgtgccagt tgttggcaat cttcggacgt gcaggtcagc
     1741 aaatcgagaa taaaccactg tggctgacgt ccagtttctt cgtttatgtg gctggctacc
     1801 tgacgtaatt tttcttcatt acggcccaac agaatcactg tcgcaccata gcgtgcatac
     1861 gtcatcgcgg cttcacggcc aataccatcg ctggctcccg tcaccaggat aatgcgatca
     1921 ttgagtaaat cttgttttgg ctggtaatgc atggctactc ctcaacgacg ttgtctgtcg
     1981 cgctgccagt gtactttatg actttggggc tttatgcctg aaacagaagg gcatttcaat
     2041 cagtctgggg cgataacacc gcaaaatgga tactttgtca tactttcgct gcaataacat
     2101 ctctgcgaga cggcttaaca tgcctgttgt aaactgtgag ccaaagcgtt gtttaaccaa
     2161 ggtggggact cgtggaattg ttgtctgaat atggtttgtt tttggcgaaa atcgttaccg
     2221 ttgtgctagc gattgcggcg attgccgcca ttattgtcaa tgttgctcaa cgtaataaac
     2281 gccagcgtgg cgagttacgg gtcaacaata tcagcgaaca gtataaggag atgaaagaag
     2341 aactggctgc ggcgctgatg gacactcatc agcaaaaaca gtggcacaaa acgcagaaga
     2401 aaaagcacaa acaagaagcg aaagcagcaa aagcgaaagc caaactgggc gaggtggcaa
     2461 ctgacagtaa acctcgcgtc tgggtgctgg attttaaagg cagcatggac gcccatgaag
     2521 tgaactcgct acgtgaagag ataacggcgg tactcgcagc attcaaaccg caggatcagg
     2581 ttgtgctacg tctggaaagc cctggtggca tggtgcatgg ttacgggctg gcggcttcgc
     2641 agctgcagcg tctgcgtgat aaaaacattc ctttaactgt tacggtagac aaagttgctg
     2701 ccagtggcgg ttacatgatg gcctgtgtgg cagacaaaat tgtttccgca ccgtttgcta
     2761 ttgtgggttc tattggcgtg gtggcgcaaa tgcccaactt taaccgcttc ctgaaaagca
     2821 aagatattga tatcgaactg cacaccgccg ggcagtataa gcgcacgctg acgttgctgg
     2881 gtgaaaatac cgaagaaggg cgggagaaat tccgcgaaga gctgaacgaa acgcatcagt
     2941 tatttaaaga ttttgtgaag cgtatgcgtc cgtctctgga tattgaacag gtggcaacgg
     3001 gtgaacactg gtacggacaa caggcggtag agaaaggcct ggttgatgaa atcaacacca
     3061 gtgatgaagt tattcttagc ctgatggaag gccgtgaagt ggtcaatgta cgctatatgc
     3121 agcgtaaacg actcattgac cgattcaccg gcagcgcggc agagagcgcc gatcgattgt
     3181 tgctacgctg gtggcagcgg ggtcaaaagc cattgatgta aaagacaaac gcgaggctaa
     3241 gacctcgcgt tttgctttaa tcaaccaggt gatatttttc tgaaagcaca tgggccaggt
     3301 gtttgaacat attaaacacc gcggtgcttt tggctgttgg caatccttgt tcatctaaaa
     3361 agtagtcgcc ggtaaatacc agcacgccat tacgctgcgt gacgccggtg gcttcaatcc
     3421 ctgcgagcgt atcctcatgc tcacgaatga ttttgttggc ctctttcaac agcgtctcgc
     3481 ggtcgatggg ttgtgtctct ttgttcatta tttactcctt aaacaaggac attagtctac
     3541 gccaggcatg gcttgcagac aaatatacca cgctggtggc aagagcgcct tactggcaac
     3601 tttggatttt gcatgctaat aaagttgcgt atcggatttt atcaggtaca gtgtgacgct
     3661 ttcgtcaatc tggcaataga tttgcttgac attcgaccaa aattccgtcg tgctatagcg
     3721 cctgtaggcc aagacctgtt aactcagtca cctgaatttt cgtgaacaga gtcacgacaa
     3781 ggggttgata tccgcagaga gcgagtccat atcggtaact cgttgccagt ggaaggttta
     3841 tcaacgtgcg acgcattcct ggaagaatca aattaggtaa ggtgaatatg ggtaaagctc
     3901 ttgtcatcgt tgagtccccg gcaaaagcca aaacgatcaa caagtatctg ggtagtgact
     3961 acgtggtgaa atccagcgtc ggtcacatcc gcgatttgcc gaccagtggc tcagctgcca
     4021 aaaagagtgc cgactctacc tccaccaaga cggctaaaaa gcctaaaaag gatgaacgtg
     4081 gcgctctcgt caaccgtatg ggggttgacc cgtggcacaa ttgggaggcg cactatgaag
     4141 tgttgcctgg taaagagaag gtcgtctctg aactgaaaca actggctgaa aaagccgacc
     4201 acatctatct cgcaaccgac cttgaccgcg aaggggaagc cattgcatgg cacctgcggg
     4261 aagtgattgg tggtgatgat gcgcgctata gccgagtggt gtttaacgaa attactaaaa
     4321 acgcgatccg ccaggcattt aacaaaccag gtgagctgaa tattgatcgt gttaatgccc
     4381 agcaggcgcg tcgctttatg gaccgcgtgg tggggtatat ggtttcgccg ctgctgtgga
     4441 aaaagatcgc tcgtggtctg tctgccggtc gtgtgcagtc ggtggcggtc cgcctggtgg
     4501 tcgagcgtga gcgtgaaatt aaagcgttcg taccggaaga gttctgggaa gtcgatgcca
     4561 gcacgaccac gccatctggt gaagcgttgg cgttgcaggt gactcatcag aacgacaaac
     4621 cgttccgtcc ggtcaacaaa gaacaaactc aggctgcggt aagtctgctg gaaaaagcgc
     4681 gctacagcgt gctggaacgt gaagacaaac cgacaaccag taaacctggc gctcctttta
     4741 ttacctctac gctgcaacaa gctgccagca cccgtcttgg ttttggcgtg aaaaaaacca
     4801 tgatgatggc gcagcgtttg tatgaagctg gctatatcac atacatgcgt accgactcca
     4861 ccaacctgag tcaggacgcg gtaaatatgg ttcgcggtta tatcagcgat aattttggta
     4921 agaaatatct gccggaaagt ccgaatcagt acgccagcaa agaaaactca caggaagcgc
     4981 acgaagcgat tcgtccttct gacgtcaatg tgatggcgga atcgctgaag gatatggaag
     5041 cagatgcgca gaaactgtac cagttaatct ggcgtcagtt cgttgcctgc cagatgaccc
     5101 cagcgaaata tgactccacg acgctgaccg ttggtgcggg cgatttccgc ctgaaagcac
     5161 gcggtcgtat tttgcgcttt gatggctgga cgaaagtgat gcctgcgctg cgtaaaggcg
     5221 atgaagatcg tatcttaccg gcagtcgata aaggcgatgc tttgacactc gttgaactga
     5281 caccagccca gcactttacc aagccgccag cgcgtttcag tgaagcatcg ttggttaaag
     5341 agctggaaaa acgcggcatc ggtcgtccgt ctacctatgc gtcgatcatt tcgaccattc
     5401 aggatcgtgg ctatgtgcga gtagaaaatc gtcgtttcta cgcggaaaaa atgggtgaaa
     5461 ttgtcaccga tcgccttgaa gaaaatttcc gtgagttaat gaactacgac ttcaccgcac
     5521 agatggaaaa cagcctcgac caggtggcaa atcacgaagc agagtggaaa gctgtactgg
     5581 atcacttctt ctcggatttc acccagcagt tagataaagc tgaaaaagat ccggaagagg
     5641 gggggatgcg cccgaaccag atggttctga ccagcattga ctgcccgact tgtggtcgca
     5701 aaatggggat tcgcacagcg agcaccgggg tattccttgg ctgttctggc tatgcgctgc
     5761 cgccgaaaga gcgttgcaaa accaccatta acctggtgcc ggaaaacgaa gtgctgaacg
     5821 tgctggaagg cgaagacgct gaaaccaacg cgctgcgcgc aaaacgtcgt tgcccgaaat
     5881 gcggcacggc gatggacagc tatctcatcg atccgaaacg taagttgcat gtctgtggta
     5941 ataacccaac ctgcgacggt tacgagatcg aagagggcga attccgcatt aaaggttatg
     6001 acggcccgat cgttgagtgt gaaaaatgtg gctctgaaat gcacctgaaa atggggcgtt
     6061 tcggtaaata catggcctgc actaacgaag agtgtaaaaa cacgcgtaag attttacgta
     6121 acggcgaagt ggcaccaccg aaagaagatc cggtgccatt accggagctg ccgtgcgaaa
     6181 aatcagatgc ttatttcgtg ctgcgtgacg gtgctgccgg tgtgttcctg gcggccaata
     6241 ccttcccgaa atcgcgtgaa acacgtgcgc cactggtgga agagctttat cgcttccgcg
     6301 accgtctgcc ggaaaaactg cgttatctgg ccgatgcgcc acagcaggat ccggaaggta
     6361 ataagaccat ggttcgcttt agccgtaaaa ccaaacagca atatgtctct tcggaaaaag
     6421 acggaaaggc gactggctgg tcagcatttt atgttgatgg caaatgggtt gaaggaaaaa
     6481 aataaccttt aattctgt
//
LOCUS       DAOTRK010000203         6481 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.204, whole genome shotgun sequence.
ACCESSION   DAOTRK010000203 DAOTRK010000000
VERSION     DAOTRK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.204"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..472)
                     /locus_tag="QIE41_004333"
     CDS             complement(<1..472)
                     /locus_tag="QIE41_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDS3533130.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENEYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKIIPIQGIKDDLQKR
                     VLPIDVYSDETDEPRLIVRKKEYDLYKYYILQDSKDYKPEKPSDTELVFISNAET"
     gene            750..3182
                     /locus_tag="QIE41_004334"
     CDS             750..3182
                     /locus_tag="QIE41_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDS3533131.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKHNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDIDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            3249..4718
                     /locus_tag="QIE41_004335"
     CDS             3249..4718
                     /locus_tag="QIE41_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDS3533132.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4718..6469
                     /locus_tag="QIE41_004336"
     CDS             4718..6469
                     /locus_tag="QIE41_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDS3533133.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     QGWEWVTLATVGEIVGGGTPKSDNPQFWAKNGIKWITPADLYGLKGKYITSGARDISP
                     AGLSNSSARLMPKGSVLFSSRAPIGYVAIADAELSTNQGFKSCVPYIKESAEYIYYFL
                     LASAKKIDAEASGTTFKEVSGAIVSKILLPLPPLSEQLKIVSRANELMSLCDQLEQQS
                     LTSLDAHQQLVETLLGTLTDSQNAEELAENWTRISEHFDTLFTTEASVDALKQTILQL
                     AVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKSLPPISDEEKPFELPEGW
                     EWSYLSDIGILARGRSKHRPRNDPTLYADGTIPLVQTGDVARSNGCINTYSALYNQLG
                     LSQSKLWNKGTLCITIAANIADSGILNFDACFPDSVVGFTPYENEIPVLYFHYFMMTI
                     KSTLEKFAPSTAQKNINIDILSQLFFPCPPLEEFHRIVDKVQNLLSVCDVLRAYIQSA
                     QQTQLHLADALTDAAIN"
BASE COUNT         1931 a         1532 c         1464 g         1554 t
ORIGIN      
        1 ttgtttcagc gttgctaatg aatactaact cagtatccga tggcttttca ggtttataat
       61 ctttagaatc ctgcagtata taatatttat aaagatcata ctcttttttt ctaacaatta
      121 gtcttggttc atcagtttca tcagaataaa catcaatagg cagaactctt ttttgcaaat
      181 catcttttat cccctgaatc gggatgattt tttttagctc ggagacaata atgcttaacg
      241 ttgtaagacg ttgttgtcca tctacaactt catactgatt ttgcccttta ttgatacaaa
      301 tcatactccc aataaaatat tcattctctt ctgattgaga gcttttataa atatcatcaa
      361 tgagttgctc tgcattatct accgtccaac tgtaaggacg ctgataagaa ggaataataa
      421 actttttgtt ttcagtaagg atatcctgca ccttacattc tttagcttcc atttcatata
      481 cccaatcaaa caatcaacaa taacattatc tttcttgctc gaaagaaacg caactataag
      541 atttttatat attttgttag agttgatatg gttcctgtaa cttctgttta tctgtctctg
      601 atatgcccag attaattcaa gcaagatata acagagatat caatgtgatc tctgctacgt
      661 actttacgtt gaaacaccgc cctaaatcat agccaggcta ttgattttat gtacaatcag
      721 aatcgtcctg ataaaagcga gaatacccca tggcggaact gaacctaagt aacctgacgg
      781 aagcagacat cattaccaaa tgcgttatgc cagccattct caatgcaggc tgggacaaca
      841 caacgcaaat cagacaggag gtcaaactcc gggacggtaa agtcatcgta cgtggtaaag
      901 ttgcggcacg cagaacggta aaatctgctg acatcgtgct gtatcacaaa ccaggcattc
      961 ccttagccgt gattgaagca aaagccaaca aacatgaaat cggcaaaggg atgcaacagg
     1021 gcattgaata tgcgcgcctg ctggacgttc cttttgtttt tgccaccaac ggtgatggct
     1081 ttatcttccg cgacgccacc gcagccgaag gtgaatgcct tgaaaagcaa ataacgctgg
     1141 atgacttccc ctcccccgct gaactctggc aaaaattctg cctctggaaa ggatacacac
     1201 aagctcagct tccggtgatt actcaggact attacgacga tggcagcggt aaatcaccac
     1261 gttattacca gcttcaggca atcaacaaaa ccattgaagc cgtctccaac gggcaaaacc
     1321 gcgttctgct ggtcatggcg accggaacag ggaaaaccta taccgcattc cagatcatct
     1381 ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt ccttgccgat cgcaacattc
     1441 tggtcgacca aaccaaacat aatgatttcc agccatttgg tacggcaatg accaaagtca
     1501 gcggacgcac cattgatccc gcttatgaaa ttcacctcgc gctctatcag gctataactg
     1561 gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc agatttcttc gatctgatcg
     1621 tgatcgacga atgccatcgc ggcagcgcat ccgaagacag cgcctggcga gagatccttg
     1681 attatttcag ttccgccacc caaattggct taaccgccac gccaaaagag acgcatgaag
     1741 tctccagcac ggattacttc ggcgatccgg tttacgtcta ctcactaaaa gaagggatcg
     1801 aagacggctt cctcgcccct tataaagttg tccgtgttga tattgatata gacctgcaag
     1861 gctggcgccc aaccaaaggg caaactgact taaacggcga agtgatcgac gatcgtatct
     1921 ataaccagaa agatttcgat cgcacgatgg taatcgacga acgcacagaa ctggttgcca
     1981 gaaccattac cgactacctc aagcgcacca acccgatgga taaaaccatc gtcttctgta
     2041 acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt taatctcaac ccggagcagg
     2101 tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga tgatgaaatt ggcaaagctc
     2161 agttggataa cttcatcaac ccgaaaaaac cgtacccggt tatcgcgacc acttcagagc
     2221 tgatgaccac cggtgtggat gctaaaacct gcaaactggt agtactggac cagaacatcc
     2281 agtcgatgac caaattcaag caaattatcg gtcgtggtac acgcatcgac gaacgttacg
     2341 gcaaactctg gtttaccatc ctcgacttta aaaaagccac cgaactgttt gccgatgagc
     2401 gtttcgatgg cattcccgaa aaagtcatgg ataccacgcc agaggatatc gccgacccag
     2461 aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca tgacgacgaa caggtaacag
     2521 gcgttgatga accgcctgcg ccaccatacc aggttaccga taccgatgat gtcggcccac
     2581 ttccggaaga agacgagaag aaaatccgca agtttcacgt caacggtgta gcggtgggcg
     2641 ttattgcgca gcgtgttcag tattacgatg ccgacggtaa actggttacc gaatctttta
     2701 aagattacac ccgcaaaaca ctgctcaaag aatatgcctc gctggatgac tttacccgca
     2761 agtggcagga cgccgatcgc aaagaagcga tcattcacga gctggaacaa caggggatca
     2821 tctgggaagt actggcagaa gaagtcggta aagatctcga cccgttcgac atgctttgcc
     2881 acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg cgccgagaac gtgcgcaagc
     2941 ggaactactt cacaaaatac tctgaagcag cgcaagccgt gctcgataat ctgctggata
     3001 aatacgccga tgcgggcgta caggagatcg aaagtattca ggtgctgaaa cttaagccat
     3061 tcgacagcat gggcacctta ccggagatta ttaaaaccgg atttggcgac cgtaacgggt
     3121 ataatcaggc gctcagcgag ctggaaaacg aaatctacca attaccgccc cgctctgctt
     3181 aacaactctc ttttgcgcct tctgcaacca tcatgttgca gaaggccttg tttttcatgg
     3241 aatacaacat gtctatcagc tcagtaatca aatcattaca agatattatg cgcaaagatg
     3301 ccggtgtgga cggcgatgcg cagcgtctcg gtcagctctc ctggctgctg tttttgaaaa
     3361 tcttcgatgc ccaggaagag gcgctggaac tggagcagga taactatcaa tatccgatcc
     3421 cacagcgtta tttatggcgc agttgggccg caaacgctca gggcattacc ggtgattccc
     3481 tgctggaatt cgttaatgat gatctgttcc cggcgttgaa aaacctcact gcgcctatcg
     3541 ataaaaaccc acgcggctac gtggtaaagc aggcgttcag cgatgcctat aactacatga
     3601 aaaacggtac gctactgcgt caggtgatca acaagctgaa cgaaattgac tttaccagcg
     3661 ccagcgaacg gcatctgttt ggtgatattt acgagcagat ccttaaagat ctgcaatctg
     3721 cgggcaatgc gggcgaattc tatactccac gcgccgtcac tcgctttatg gtggatcgcg
     3781 ttgatccgaa actcggcgaa tccattatgg acccggcctg cggtacgggc ggttttcttg
     3841 cctgcgcatt tgatcatgta aagaacaaat acgtgaagag cgtcgccgat catcagacgc
     3901 tgcaacaaca gatccacggt gttgagaaaa aacagcttcc gcacctgctg gcgaccacca
     3961 atatgctgct acacggcatt gaagtgccag tgcaaattcg tcacgacaac actctgaaca
     4021 aaccgctttc ctcctgggat gagcaactgg atgtcattgt taccaacccg ccgtttggtg
     4081 gcacggaaga agacggtatt gagaagaact ttccagcaga gatgcaaacc cgcgaaacgg
     4141 cggatttgtt cctgcaactg attgtggaag tactggcgaa aaatggtcgc gcggcggtgg
     4201 tattgccgga tggcacacta tttggcgaag gcgttaaaac caaaatcaaa aagctactta
     4261 ccgaagagtg caacctgcat accatcgtgc gcttaccgaa tggtgtgttt aacccctata
     4321 ccggcattaa aaccaacctg ttgttcttta ccaaaggtca gccaaccaaa gagatttggt
     4381 tctatgagca tccgtatccg gcgggcgtga aaaattacag taaaactaag ccgatgaagt
     4441 ttgaagagtt tcaggcggag attgactggt ggggtaacga ggccgatggt tttgccagcc
     4501 gcgtcgagaa tgagcaggcg tggaaagtca gcattgatga cgtgattgcg cgcaacttca
     4561 atctggatat taaaaaccca catcaggcgg aaaccgttag ccatgatccg gacgaactgt
     4621 tagcgcagta tgcaaaacag caggcggaga tccagacgct gcgtaatcaa ctgcgcgata
     4681 ttcttggcgc tgcgctgtct ggcaaggagg ttaactaatg agtgtggaaa agctgatcgt
     4741 tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt tccaccgccg ggcgcggcag
     4801 ttccggaaaa attgatttat atggcattaa gaaattacgt gagctgattc tggaactggc
     4861 tgtgcgcggt aaactggtgc cgcaggaccc gaacgatgaa ccggcgtcgg agctgctgaa
     4921 gcgtattgcg gcagaaaaag cggaactggt gaaacaggga aaaattaaaa aacaaaaacc
     4981 actgccggaa attagcgagg aagagaagcc gtttgaattg ccgcagggat gggagtgggt
     5041 cacactggca accgtaggtg aaatagtcgg aggaggaact ccaaagtccg ataatccgca
     5101 attttgggcc aaaaatggta ttaagtggat tactccagca gacctctacg gactcaaagg
     5161 gaagtacatt acatctggag cccgtgatat ttcacccgca ggactgtcaa actcatcagc
     5221 ccggttgatg ccaaagggga gtgtgctttt ctctagccga gcaccaattg gatatgtcgc
     5281 tatagcagat gcagaactgt caacaaacca gggttttaaa tcttgtgttc catatatcaa
     5341 agaaagtgca gaatatatat attactttct actggcatct gcaaaaaaaa tagatgcaga
     5401 ggcaagtggg actacattta aagaagtctc tggggcaatt gtttcgaaaa tattattacc
     5461 tttaccgcca ttgagtgaac aactaaaaat cgtctcaagg gcaaatgaac ttatgtccct
     5521 atgcgaccaa ctggaacagc aatccctgac aagtctggac gcacatcagc aactggttga
     5581 aaccctgttg ggaacactta cagacagcca aaacgccgag gaactggctg aaaactggac
     5641 gcgtattagc gagcatttcg acacactatt taccaccgaa gccagcgtgg atgcgttaaa
     5701 acagaccatt ctgcaactgg ccgtaatggg taaacttgtg ccgcaggatc cgaatgacga
     5761 accagcctcc gaactgctca aacgtattgc gcaggaaaaa gctcaactgg tgaaagaagg
     5821 gaaaataaaa aaacaaaaat cgttgccacc gattagcgat gaggaaaaac cgtttgaact
     5881 tccggaaggg tgggagtgga gttacctatc agatattggc atacttgcta gaggaagatc
     5941 taaacataga cctcgtaacg atcccactct ttatgctgat ggcacaatcc cattagtaca
     6001 aacaggtgat gttgcaagat ctaatggatg tataaataca tattccgcgc tatataatca
     6061 actaggatta agtcaaagta aattatggaa taaaggtact ctttgtatta cgattgctgc
     6121 aaatattgca gactctggta ttttaaactt tgatgcttgt tttcccgata gtgtagttgg
     6181 ctttactcca tatgaaaatg aaatacctgt tctttatttt cattatttca tgatgacaat
     6241 aaaatccact ctggaaaaat tcgccccatc aactgcgcaa aaaaacatca acattgatat
     6301 attaagtcaa ttgtttttcc catgtccacc attagaagaa ttccatcgta ttgttgataa
     6361 agttcaaaac ttattatcag tttgcgatgt tctaagagcc tacattcaat ccgcccagca
     6421 aacccaactg caccttgcag acgcactcac tgacgcggcg ataaactaat tttctcgccc
     6481 t
//
LOCUS       DAOTRK010000204         6391 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.205, whole genome shotgun sequence.
ACCESSION   DAOTRK010000204 DAOTRK010000000
VERSION     DAOTRK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.205"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            92..355
                     /locus_tag="QIE41_004337"
     CDS             92..355
                     /locus_tag="QIE41_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDS3533134.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            456..1097
                     /gene="lipB"
                     /locus_tag="QIE41_004338"
     CDS             456..1097
                     /gene="lipB"
                     /locus_tag="QIE41_004338"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDS3533135.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            1357..2310
                     /locus_tag="QIE41_004339"
     CDS             1357..2310
                     /locus_tag="QIE41_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDS3533136.1"
                     /translation="MDSNNQIETCLSRKSSEDKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKSHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLL
                     PEGQNFSGLRQRVQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            2519..3484
                     /gene="lipA"
                     /locus_tag="QIE41_004340"
     CDS             2519..3484
                     /gene="lipA"
                     /locus_tag="QIE41_004340"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDS3533137.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(3586..3789)
                     /gene="tatE"
                     /locus_tag="QIE41_004341"
     CDS             complement(3586..3789)
                     /gene="tatE"
                     /locus_tag="QIE41_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDS3533138.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(3918..4706)
                     /locus_tag="QIE41_004342"
     CDS             complement(3918..4706)
                     /locus_tag="QIE41_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDS3533139.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            4802..5185
                     /gene="crcB"
                     /locus_tag="QIE41_004343"
     CDS             4802..5185
                     /gene="crcB"
                     /locus_tag="QIE41_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDS3533140.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(5239..5448)
                     /gene="cspE"
                     /locus_tag="QIE41_004344"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(5239..5448)
                     /gene="cspE"
                     /locus_tag="QIE41_004344"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDS3533141.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(5623..6183)
                     /gene="pagP"
                     /locus_tag="QIE41_004345"
     CDS             complement(5623..6183)
                     /gene="pagP"
                     /locus_tag="QIE41_004345"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDS3533142.1"
                     /translation="MNVSQYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1707 a         1584 c         1410 g         1690 t
ORIGIN      
        1 tgtaatttcc gtccccatat actaagcatc agtaaaaaac tcccgccttc tggcgggagt
       61 tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga
      121 attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca
      181 ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag
      241 cagcaaaggc aactaccact cggtatctat cactatcaac gctactcata tcgagcaggt
      301 tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc
      361 ttctccgtta cccggctcct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc
      421 ccccactttt actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc
      481 gccagctcgg tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata
      541 cccgcgatga aagtaccctt gatgaaatct ggctggtcga gcactatccg gtatttaccc
      601 aagggcaggc aggaaaagcg gagcacattt taatgccggg tgatattccg gtgattcaga
      661 gcgatcgcgg tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtgttgc
      721 ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag
      781 tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg
      841 tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat
      901 tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt
      961 gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta
     1021 ataatattgc accgcgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg
     1081 aatatattac cgcttaattg catacatcaa tggcccaatt tacactgggt cattactcat
     1141 aatacctgct aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga
     1201 aatgcgatgc tacgccttgc tccgtaactc accccattga ttaacttatt gaaatagttg
     1261 attatggtga gcaaaagcgt tccgttccaa ttagacataa gattattgta atttttttta
     1321 atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa tcaaattgaa
     1381 acctgtttaa gcagaaagtc gtcagaagat aaaccacaaa tatttacaac cttacgaaat
     1441 attgatctta accttctgac tatttttgaa gctgtatatg tacataaagg gatcgttaat
     1501 gcagcgaaag tgcttaatct gacaccctcg gcaatcagtc agtctattca gaaactgcgc
     1561 gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc taccgcattt
     1621 gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg cgcgctggat
     1681 atcgaaggaa gctatgataa gcaacgaacg ataaccattg cgaccacccc ctcggtcgga
     1741 gccctggtcc ttcctgtcat ctatcgggcg attaaatctc actatccgca gcttttactg
     1801 cgcaaccccc ccatcagcga cgcggaaaac caactcagtc agtttcaaac cgatctcatc
     1861 atcgataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt caccgacaat
     1921 atggtgttaa tttgccgcga gggaaatcca ctactctctt tagaagatga cagggagact
     1981 atcgacaacg ctgcgcatat actcctgtta ccggaagggc aaaatttcag cggtctgcgg
     2041 cagagagttc aagagatgtt tccggaccgg caaatcagtt tcaccagcta caacattttg
     2101 acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag ccgtttttat
     2161 aacctgttta gccgttgctg gccgctggaa aaactgcctt ttccgtcctt aaatgaggag
     2221 caaatagatt tctctatcca ctacaacaaa ttcagcctgc gtgatcccat cctgcacggg
     2281 gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcga
     2341 agaacgaaaa aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc
     2401 tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac
     2461 aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat
     2521 gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct
     2581 tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat
     2641 gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa
     2701 aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacttggcgg aatgcttcaa
     2761 ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt gtccgttctg
     2821 tgacgttgcc cacggtcgcc cggtagctcc ggatgccaat gaaccagtga aactggcgca
     2881 gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgacct
     2941 gcgcgatggc ggtgcccaac actttgcgga ttgcattact gccattcggg aaaaaagccc
     3001 gcaaatcaaa attgaaactc tggtgccgga tttccgcggt cgtatggatc gtgctctgga
     3061 tattctgacc gccacaccac cagatgtatt caaccataac ctggaaaacg taccgcgtat
     3121 ttaccgtcag gtacggcctg gtgcggatta caactggtcg ctgaagctgc tggaacgctt
     3181 taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggtc tgggtgaaac
     3241 caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgctaac
     3301 gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc
     3361 ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc atgctgcatg
     3421 cggtccgttt gtccgctcgt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa
     3481 gtaatcctgt aataattact tacatctatc tgataaaaaa cccgcttttt gaagcgggtt
     3541 tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact ctttatgaga
     3601 gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat cgtcatcatt
     3661 catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg tacgtaactt
     3721 cttagtccca aacagcagaa cgaccagcgc cgcaactacc agcagtttgg taatactaat
     3781 ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt aaaaaccaga
     3841 gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag gtgaatcaag
     3901 cgccgagtaa aaaaacatca taataattgc ggcggcgcaa agcgacggtt gtttaaaacg
     3961 ggaagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat aatgagtgca
     4021 ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg gctttgcccg
     4081 atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc cagcgcacga
     4141 gcggcaagca acgttgacca gtgatgctct ttgagcggcc cacgaaccca ggcggcagga
     4201 agtaccagga tttcagctcc ctgtaatgcc tgtgccagcg ccagctctgg aaagcgtaag
     4261 tcataacagg tcatcagacc gaccttcatc ccttccacct ccagtaacgg agctatttca
     4321 ttaccagcat caacatggcg tgattcctga atggcaaatg catcatagag atgcagtttg
     4381 gcataacggg cgacgatgtt tcctgcctgt agcgccacca gcatattcca tgcgcgcccc
     4441 ggcgttgaag gaacatgaat cgtcagaatt gtcgtcatca tgttacgttt actttctcgc
     4501 cgtaaacgtc cgaggaattc gccttccagc agctgtgctg atttaaccga tagatctgca
     4561 tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa acagcgatac gtcgttttcc
     4621 gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct tttcccacac agatgtaaca
     4681 gcaaactgtc ctgcggcaac cagcattgcc acctcctcct gtcagagtcg taaaattcgc
     4741 caacggcgat tattcggcgt tacacttatc actcatacaa atcaaatagc aggattttgc
     4801 agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg tggcgagatg
     4861 gctgttaagt atgcgattta acccgctgca tcaagcgatt ccgttgggga cgctggctgc
     4921 aaacctgatt ggggcattca tcataggaat gggattcgcc tggtttagca ggatgacgaa
     4981 cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc taacaacctt
     5041 ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg gctgggcgtt
     5101 actgaacgtt ttcgtcaacc ttttggggtc ttttgccatg accgcactgg ctttctggct
     5161 gttttccgcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa gcgggttttg
     5221 aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc ctttggcacc
     5281 gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac cattagtctg
     5341 gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa tgaaaccgaa
     5401 tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca aaattacctt
     5461 tacatgaaaa atagacacaa atgctgtgtc ggttaccagt acaccaattg tggtacgctt
     5521 tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat gttacttatt
     5581 ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact gaaagcgcat
     5641 ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca tctgaaaagt
     5701 cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga tgtaattcca
     5761 gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat tttcatccgc
     5821 cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt tgttccacga
     5881 gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc aacgcgactg
     5941 accaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt tgtcgtaagc
     6001 gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag gctgttgcca
     6061 ggtttgtgca atattttctc taaacgttgt catccactca tctgcgttag caaaaacttt
     6121 accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt gactcacgtt
     6181 catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa gctacaatta
     6241 acataacctt aatagcccca acatgaagaa taatctgaat agctgctcta tttatttccg
     6301 cttgttcaag ataaatattt ccgcgcatcg attaaagatg attcgtcgtt ttctgcgatg
     6361 ggaatagtca aaaaagaaaa accaagtatt t
//
LOCUS       DAOTRK010000205         6039 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.206, whole genome shotgun sequence.
ACCESSION   DAOTRK010000205 DAOTRK010000000
VERSION     DAOTRK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.206"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1380)
                     /locus_tag="QIE41_004346"
     CDS             complement(<1..1380)
                     /locus_tag="QIE41_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDS3533143.1"
                     /translation="MSRIESDFLGEREIPDDCYYGVQTLRGKDNFHITEMPMSQEPFF
                     IIAFGYVKKAAAMANKELGTIPADVADALIWACDQLIDGKYREQFVTDWLQGGAGTST
                     NMNCNEVICNLAAEKLGSVKGDYKRVSPNDHANFGQSTNDTYPTALHLALLLRSNVLL
                     EAVEHLVGAFYKKADEFSTVLKMGRTHLQDAVPMTLGQEFHGWGFTINDEIRVIRNAQ
                     EHLRVVNLGATAIGTCVTAHPDYPALAVKYLAQITGINFRNSEDLIAATSDCGAYVAL
                     SSAMKSLSVKLTKVCNDIRLLASGPRCGLAEINLPQLQPGSSIMPGKVNPVIPEVTNQ
                     SCFLVQGLDTTVMLAASAGQLELNVMEPVITFALFTSLKVMTNACNTLRTKCIDGITA
                     NSDRTAEMVMHSCGIVTLLKPHLGYKVCSEMAHEAYHTGKSLHQIVVVERKLLTQEEW
                     EKTFNLDNLI"
     gene            complement(1603..2901)
                     /locus_tag="QIE41_004347"
     CDS             complement(1603..2901)
                     /locus_tag="QIE41_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDS3533144.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRLASNFMRRNPKYINIIAPIITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFVAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNNSI
                     KPGATLSVGLFMLATIAIVILAAFPQLRPGFDISKPMETRDIIIICMLSAACLMVILC
                     KMSTDDIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQTYPMLLAL
                     VLFFTCALLYSQGATTPLIIPLAVALNIPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGNYLLNHPFMLPGLGGVIAGVTFGFVIAPMMV"
     gene            complement(2960..3679)
                     /locus_tag="QIE41_004348"
     CDS             complement(2960..3679)
                     /locus_tag="QIE41_004348"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDS3533145.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQRDQEHIPLIISSIP
                     DIPDRTDALMHNGLSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIIDTTVDEVRVCGKTRIGLLATDATLYMGLYQKGIESLGFNYITPDADGQKNVMKSI
                     YSLKAGNVTHAQKLMEEQAENLFSRGAEILILGCTEIPIILSQAVKDQPLRYIDSTAS
                     LVRAGIKWYENRIGKDQHLTQ"
     gene            4019..4918
                     /gene="hypT"
                     /locus_tag="QIE41_004349"
                     /gene_synonym="qseD"
     CDS             4019..4918
                     /gene="hypT"
                     /locus_tag="QIE41_004349"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDS3533146.1"
                     /translation="MKNIETKWLYDFLTLEACRNFCQAAKDRNLSQPAFSRRIKALES
                     AIGVVLFDRTTSPLQLTEEGRLFHSQTRSLVQQLECNLNELGGDNSFNLPNIKIAAAH
                     SLSLSMLPKLLHSLTTLGGDFIYHVEAIDVVQAVNTLREGKSDFIISFRDEEFMQSPF
                     CCMKLFESELYPVCAADQQGKPIFDLTNSQAPLLDYTPTSYMGRLVNRHLAEQGGIAG
                     HTILVSSMSELLKNMALNGLGIAWLPLWSIADELNTKRLVCPWPAKWAVPIQAYIYRM
                     DTRLNKTAEHFWTILRNNIKENS"
     gene            5578..6039
                     /gene="tnpA"
                     /locus_tag="QIE41_004350"
                     /pseudo
     CDS             5578..6039
                     /gene="tnpA"
                     /locus_tag="QIE41_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1827 a         1271 c         1294 g         1647 t
ORIGIN      
        1 aatcagatta tccagattga atgtcttctc ccattcttcc tgtgtgagta gcttacgttc
       61 gacaaccact atctgatgga gggatttgcc tgtatggtat gcttcgtgcg ccatttcaga
      121 acacacctta tatcccagat gtggcttcag gagagtcaca ataccgcagg aatgcattac
      181 catctctgca gttcgatcgg aattagctgt aatgccatca atgcatttag ttcggagtgt
      241 gttacaggca ttcgtcatca ccttgagtga ggtgaacagc gcaaaggtga tgaccggctc
      301 cataacgtta agctcaagct gaccagcaga tgccgccagc atcactgtgg tgtccagccc
      361 ctgaaccagg aagcaagact ggtttgttac ttccgggata acggggttaa ccttaccagg
      421 cataatggaa gaacccggtt gcaattgagg caggttaatt tcagccaggc cgcaacgggg
      481 gcctgaagca agcagtcgaa tgtcattaca aaccttggta agcttcacag agaggctctt
      541 catggccgaa ctgagtgcaa catatgcacc acagtcgctt gtggcagcga tgagatcttc
      601 actgttcctg aaattaatgc ctgtgatctg ggccagatat ttcacggcta aagcgggata
      661 atccgggtga gcggtaacac aggtaccaat cgcagtagca ccaaggttta caacccgcag
      721 gtgttcctgt gcattacgga tgacccgaat ttcatcatta attgtaaaac cccagccatg
      781 gaactcctgg ccgagagtca ttggcactgc atcctgcagg tgagtacgtc ccatcttgag
      841 cactgtgctg aactcgtcag cctttttgta gaatgctcct acgaggtgtt caacagcttc
      901 aagcaataca ttgcttcgca gcagcaatgc cagatgtaat gctgtcggat atgtatcatt
      961 ggtggactga ccaaaattag catggtcgtt tggcgaaacg cgtttgtaat cccctttaac
     1021 gctaccaagt ttttctgcag caagattaca aatcacttca ttgcagttca tattggttga
     1081 cgttccggct cccccctgaa gccagtcggt cacaaactgt tcccggtact taccatcaat
     1141 taattggtcg caagcccaga ttaaagcatc cgcaacatca gcaggaattg tacctaactc
     1201 cttattagcc atcgcagctg cttttttcac ataaccgaag gcaatgatga agaaaggctc
     1261 ctgactcatt ggcatttcag tgatatggaa attatctttt ccacgaagtg tctgaacacc
     1321 ataatagcaa tcatccggaa tttcacgctc accaaggaaa tcgctttcaa tacgagacat
     1381 aatattctaa ctccattatt tgaaaacaat gtgacaataa caaattgttt aaccggagct
     1441 atgctctcgg aatttgaatt cttacatcat tgaaataaca tgtgatatca atctgtcaga
     1501 attcttctga cagtcagtaa ctgaatgcag attaactgcc tgactaatta tcaaatagta
     1561 actctgtaac tacttccata aagaatgaaa attatgattc tatcagacca tcattggtgc
     1621 aatcacaaac ccaaatgtca ctcctgcaat aacaccaccc aacccgggta gcataaatgg
     1681 gtgatttaat aaataattgc ccacacgggt tgtgccggta gtatcaaatt ccatcgcggc
     1741 gagtgatgtt ggatacgttg gaagcacgaa tactcccgta acggcaacat aagatgcaag
     1801 aattgcccag gttggtatgt tgagtgctac agccagagga ataattaacg gcgtagtcgc
     1861 tccctgtgag taaagtaaag cgcaggtaaa aaaaagaact aatgctaaaa gcatcggata
     1921 agtttgtaaa atatcaccag cgatatcttt gatctcagtt aaatgcgcat caataaaggt
     1981 ggtacctaag gttacaatac caagaatcac tgccagcgaa ctcattccag cacgaaatgt
     2041 ggaggtcaga ataatgtcat cagtagacat cttacacagt attaccatca ggcaggcagc
     2101 agagagcatg caaataataa taatatcgcg cgtttccatc ggtttgctga tatcaaaacc
     2161 cggacgcaat tgggggaatg cagcaagaat aacaatggcg atcgtcgcca gcatgaataa
     2221 tccgacggaa agcgttgcac caggtttgat gctgttatta ttctcgtatt tttgcaccag
     2281 acctttttgc agacgttcaa gatatacttc gtcatcttgt aattcacacc cctgtcggga
     2341 ggcgataaaa gcagcaacca tcgcggcgac aaaagaggtg ggaaggcata ccgacatcac
     2401 ctgaataaaa ctgacgccat taacctccat aatagtgagc atagctgcca ttgcggcact
     2461 gattggtgag ccagaaattg caacctgtga ggcgacaact gagccagcga gtgtgcggga
     2521 aggccggata ccggactctt ttgctacttc agcaattacc ggcagtgtgg agaaaacaat
     2581 aaacccggta ccggccataa tggtcattaa ccaagtgata atcggtgcga ttatgttgat
     2641 atattttggg ttacgtcgca tgaaattact ggctaaacgt accagataat ccatcccacc
     2701 tgccgcctgt aaggctgaag ctgccacaac tacggtcata ataatcagga tcacatcgac
     2761 tggcggtgat cccataggca gaccaaatcc cagagtcagg atcgcaaggc caagaccgcc
     2821 gcataaacca atgccgattc ctccctgacg aataccaaaa aatattgcgc caagtacaac
     2881 gataatttcc agccaaacca tgatgttctc cgttgtaaat ataatctttg taaaataact
     2941 ttctggctgt gatttagaat tattgagtta aatgttgatc ttttccgata cgattttcat
     3001 accattttat tcctgcgcga acgagtgaag cggttgaatc aatatagcgt aaaggttgat
     3061 ctttaacggc ttgtgacaga ataatgggaa tttccgtaca accaagaatc aagatctctg
     3121 ctccgcggga aaataaattt tcagcctgct cttccatcaa tttttgcgca tgtgtaacat
     3181 ttcccgcttt aagtgaataa atgcttttca tcacattttt ttggccatct gcatccgggg
     3241 tgatatagtt aaaccctaac gattcaattc ctttctggta taatcccata tacagcgtgg
     3301 catcagtggc caggaggcca attctagttt taccacagac gcgaacttca tcgaccgtag
     3361 tatctatgat acttagtata tcaatatggc aagcatcttt tagttcttta aaccaatagt
     3421 gtgcggtatt acagggaata acaatacatt ctgctcctgc atcttccagt ttatgcatat
     3481 agtcacgcat aacgggtaaa ggcgagagcc cgttatgcat taatgcatcg gtacggtcag
     3541 gaatatccgg gatagaagaa ataattaaag gtatatgttc ttgatctcgt tgtgctgctg
     3601 taaaggtgac aaatttatta aataagtcga cagtggcggc cggccccatt ccaccgagca
     3661 caccgattaa acctttcaca atacttacct ctcagaaaat caatgctcat aaacttaaaa
     3721 aaccatttct ccttttttaa cagtctctaa aggctatgag aaatgcaatg atgtacaatg
     3781 caatgcaaga cttgcatgat gtagttttgc aatatacatg aaccatatac tattgttgca
     3841 tgaagaccta tcataatgat gaaaataaac gcttttggga gaagtgttaa actggtttag
     3901 atacagaaaa cgaatggatt atgcataaaa cgaataactt aaatatgcta tctgcattca
     3961 ttaccccggt tttcgggggg tatactaact aaaatttgat aatacttgga taatagaaat
     4021 gaaaaatata gagaccaaat ggttatatga tttcctgaca ctggaagcct gtcgcaattt
     4081 ctgtcaggca gcaaaagatc gtaacctttc acaaccagca ttcagcagac gaataaaagc
     4141 gttagagtca gctataggtg ttgttttgtt cgatcgtaca acctctcctt tacaattaac
     4201 tgaagagggt aggctttttc actcccagac acgcagttta gtgcaacagc ttgaatgcaa
     4261 tctgaatgag ttaggggggg ataattcatt caatttgcct aatattaaga ttgcagctgc
     4321 gcactcatta tccctcagca tgctacctaa acttctgcac tcactcacca ccttaggcgg
     4381 agactttatc tatcatgttg aggctattga tgtcgtccag gccgtaaata cgttacgaga
     4441 agggaagagt gactttatta tttcatttcg agatgaggag tttatgcaat cgccattttg
     4501 ttgtatgaaa ctgtttgaat ctgagttgta tccggtatgt gctgctgatc aacagggaaa
     4561 accgattttc gatcttacca attcacaggc tcctttgctc gactatactc caacttctta
     4621 tatgggcaga ctggttaatc gtcatttggc agaacaggga gggatagccg gacacacaat
     4681 attggtatct tcaatgagtg aactactaaa aaatatggca ctcaatggac ttggtattgc
     4741 ctggctccct ctctggtcaa tcgcggatga actgaacaca aaacgacttg tgtgtccgtg
     4801 gcctgctaaa tgggcagtgc ctattcaggc ttatatttat cgtatggata ctcggctgaa
     4861 caaaacggct gaacactttt ggacgatttt acgaaataac attaaagaga atagctagaa
     4921 acatcgtgga cagtttttgt tgagcacgac aaatccacgt ttttctgggg gtgaaggggg
     4981 ggaattcgtt aaactggatg tcttttgctt taatcagttc atctccttca ggataattct
     5041 ggccattgag atcactttga gaggtatttt tttctctttt cacatagtaa ataataacat
     5101 tgtttaatta ataacagaaa atatactaaa atcaataatt gtaataataa attatttgtt
     5161 gtcccagata agttaagtgt cattaaaatg ctggacaatc ctcgcgtaac ggtatttaat
     5221 gactacagca cgttccagga aaagcagtgg aagcaaatat tggtcattat gtcgaaaaca
     5281 ccggaaacgt actctatatt gtgtagtcat ttattcaaat gacagcgtat aaacaaattg
     5341 atcagaatca catttttttg tgaaaatcat tgtcgagagg caacatttca tagtaagtgt
     5401 atcctacagg tcatgtataa aacctacatc atgtaaacaa actcatcgaa ttgcacctcc
     5461 tgtttgtgga ggtgcttttt tgtgcacagc tccccagcca ggctgggggt tccggaaagc
     5521 tttcagtttt gagccagtta ttaaaacctc ttttgatttg ttaaaacacc ttgcggtatg
     5581 gcaacagcaa gtgtcaaaca aaaaatcaaa agggggaccc aatggggaac gaaaagagct
     5641 agcgcacccc gatggaactg taaatatcac atagtttttg cgccaaaata ccgaagacaa
     5701 gtgttctaca gagagaagcg tagagcaaca ggcagtattt tgagaaagct gtgtgagtgg
     5761 aaaagcgtac ggattcttga agctgagtgc tgtgcagatc atatccatat gcttgtggag
     5821 atcccgccca aaatgagcgc atccggcttt atgggatatc tgaaagggaa aagcagtctg
     5881 atgctttacg agcagtttgg tgatttgaaa ttcaaataca ggaacaggga gttctggtgc
     5941 agtgggtact aagtcgatac ggtgggtaag aacacggcga agatacagga ttacataaag
     6001 caccagcttg aagaggataa aatgggagag cagttatcg
//
LOCUS       DAOTRK010000206         5934 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.207, whole genome shotgun sequence.
ACCESSION   DAOTRK010000206 DAOTRK010000000
VERSION     DAOTRK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.207"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..2100)
                     /locus_tag="QIE41_004351"
     CDS             complement(<1..2100)
                     /locus_tag="QIE41_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS3533147.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGAMGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPLTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTSGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            2342..3403
                     /locus_tag="QIE41_004352"
     CDS             2342..3403
                     /locus_tag="QIE41_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDS3533148.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3516..5015)
                     /gene="ansP"
                     /locus_tag="QIE41_004353"
     CDS             complement(3516..5015)
                     /gene="ansP"
                     /locus_tag="QIE41_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDS3533149.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEQQEIVFKPETAS"
     gene            5282..5888
                     /locus_tag="QIE41_004354"
                     /pseudo
     CDS             5282..5888
                     /locus_tag="QIE41_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
BASE COUNT         1559 a         1526 c         1441 g         1408 t
ORIGIN      
        1 ctcaaatcgc catgcaatat tcatgccgac accataattt cgcccgggcg caggttcgta
       61 atatcgcccg tttgactcat tgacaatgac agaaccaacg tattctttat cgaataaatt
      121 atcgacacga ccaaataaat ccacagttaa attgtggtaa ttgtatttat acccggtgaa
      181 taagccgacg agagtataag acggcgcttt ggccgtattt tcatcatctg ccataatatc
      241 gcccatataa cgcgcttccg tacctgcata ccagccatcc tccggtacat aacctatcga
      301 cgcaaagccc atattacggg cgatccctgg catccgatta ccgttacaat cctgttcatt
      361 gcaaacattg ctgcgatagg tcgcatcgag ccaggtccag gacgctttta cacgaaaatc
      421 tcccgcgaaa cgttgatccc atgccagctc agcgccttga cgacgggtct ttccggcatt
      481 tttgtaagtc gtacgcccac cgctactgct atcgactaca atttcatcat cggtgtcggt
      541 ctggaacaat gcgagactaa gcagcccatc accaatacgc gttttactgc cgatctcaat
      601 tgtatcgttg gtggatggtt ttaaaccaaa gttcataccg ctttgaccgt cagcacgata
      661 agacagctca ttaatcgtcg gcgtttcaaa acctcgcccg gctgccagat agatattcca
      721 ggcatcggtc attgcatatt ttaacgaacc ggcaggtagc catttgtgat aactggcatc
      781 accgctgtca tcgccgttac ccgaagtaac gtaatggtcg ttggaatcaa accatacgga
      841 gctgtagcgc acgccagcat ccagcgacag tttttcgctc agctgccact gtgtctgcaa
      901 atagggatcg acgttccaca tcagattgcg ttcgtcacgg cgcaactcac ctttttgccc
      961 gtattccggc acgccgctat tcaggcggaa gttattgtag cctttgcgat tttcactcat
     1021 gttttcgtag ttcaggccgg tagtgaacgt gagcggaacg cccagctcgc cacggtgtgt
     1081 ccagcggctg tctatcccct gataatggcg ttgcagagta atcacgccgc ccgcatgcga
     1141 cgggttaagt tgtggtgcca tcgggattga ctggtactgg gtcgtttctc gctccccggc
     1201 atacatcatc acactcatat catcttgtgc gctcaggcta cgttcatagc gcaacccagc
     1261 ctgagtttgc ttgatggttt ttcgcgtgtc gtactgttca gcgcgtggcg cttgttgcgg
     1321 attcgccttc cattctgctt cggttagccc acctgggtca tctgctttga tatccacact
     1381 attgaaaatc aggcttaatt tgctggcgtc atcaatgcgt acgcccagtt tggcattggc
     1441 taaatttttc tgtgcgccac tatgatcacg atagccgtgg gtcgtaaagc gcgtggttga
     1501 gacggtgtaa tcgacatcgc caggctgagt gccgtcgccc attgcgcctg ttgctttcag
     1561 cccatagcgc cagctgccaa aactgccgta gtaactactg gcttcaatgg ttggtggctg
     1621 ttgtccggtc tgggtgatga cattcattac accgccagac gcgttgccat acagagcaga
     1681 gaaggggccg cgcagcactt ccacattttg tacactgctt aaatcgatgt tggatgtttg
     1741 cccttgtccg tcgggcatgg tggcgggaat accgtccaca tacaggcgaa taccgcgaat
     1801 tccgtaagtg gagcgggagc caaatccgcg aatcgacagc tgtaaatctt gcgcatagtt
     1861 ctgccggttt tgtacctgca aaccaggcac gctggtcagt gattcggaca agttaatgcg
     1921 cggtgttgcc aggcgcatct cctcgccatc caccacgctc acggctgctg gggtatccag
     1981 ttctgaaacc acctgcggtg cggcactgac aatcatggtc tgttcatcag cggcaaaaac
     2041 aacgggggaa aggacaagca gtgcgggcaa aacggtctgt cggacggaaa aaatcttcat
     2101 gaaaaaagcc aagttaagaa tgggaaaacg ccgccatggt aataaaattg taaatatatg
     2161 gaaaacgaaa cggcacgata cgttaagtaa ttgagaaaat tgtagtcgta acggcaagaa
     2221 atgctccaca tttgagaaaa taatgattac cattcccatt tataacaaga gcgtaacgat
     2281 gattacgctt agcgaagcat tgtgaagcag caaaaatatc ggttcatcaa agggagtcgt
     2341 catgcattta cgtcatctgt tttcatcgcg cctgcgtggt tcattactgt taggttcatt
     2401 gcttgttgct tcatcattca gtacgcaggc tgcagaagaa atgctgcgta aagcggtagg
     2461 taaaggtgcc tacgaaatgg cttatagcca gcaggagaac gcgctgtggc tcgccacttc
     2521 gcaaagccgc aaactggata aaggcggcgt ggtttatcgt cttgatccgg ttactctgga
     2581 agtgacgcag gcgatccata acgatctcaa gccgtttggt gccaccatca ataacacgac
     2641 tcagacgttg tggtttggta acactgtaaa cagcgcggtc acggcgatag atgccaaaac
     2701 gggcgaagtg aaaggccgtc tggtgctgga tgatcgtaag cgcacggaag aggtgcgccc
     2761 gctgcaaccg cgtgagctgg tagctgatga tgccacgaac accgtttaca tcagtggtat
     2821 tggtaaagag agcgtgattt gggtcgttga tggcgagaat atcaaactga aaaccgccat
     2881 ccagaacacc ggtaaaatga gtaccggtct ggcgctggat agcaaaggca aacgtcttta
     2941 caccactaac gctgacggcg aattgattac catcgacacc gccgacaata aaatcctcag
     3001 ccgtaaaaag ctgctggatg acggcaaaga gcacttcttt atcaacatta gccttgatac
     3061 cgccaggcag cgtgcattta tcaccgattc taaagccgca gaagtgttag tggtcgatac
     3121 ccgtaatggc aatattctgg cgaaggttgc ggcaccggaa tcactggctg tgctgtttaa
     3181 cccggcgcgt aatgaagcct acgtaacgca tcgtcaggca ggtaaagtca gtgtgattga
     3241 cgcgaaaagc tataaagtgg tgaaaacgtt cgatacgccg actcatccga acagcctggc
     3301 gctgtctgcc gatggtaaaa cgctgtatgt cagtgtgaaa caaaaatcca ctaaacagca
     3361 ggaagcaacc cagccggatg atgtgattcg tattgcgctg taatagatat ggcgagggtg
     3421 aaaacggtga gacttgcctg acttttatca cgccgtatcc ggcaccttcg tcagtaattt
     3481 gaatttgccc ggcacgtagc cgggcaattt tgcaattaac tcgccgtttc aggcttaaac
     3541 acaatttcct gttgttcttc atcttcctcg acgactggcg cagtgctgtg aatttcagca
     3601 acgcgtttgc gcacaccaaa ccagcctata accagcagaa taccgataat cggcagcgcc
     3661 gcgatggtat aagtcccgtt cgggtaatcg aacgccatca gcaccaggac gctcagcaaa
     3721 aacagtaatg tcagccagga agtgaagggc gcgccaggca gtttaaaact gacatccgcg
     3781 gctttgcctt ctttaatcgc tttacgcagg cgcatctggc acacgatgat aaacgcccat
     3841 gaggcgatga ttcccagcga cgcgaagttc aacacaatct caaatacgcg cgacggcacc
     3901 agatagttga ggaatacgcc gacgacatac acaaccagtg tcgccagaat cccggcatac
     3961 ggcacatgct gacgactcat tttcgccata aaactcggtg cggaaccgcc catcgccatt
     4021 gagcgcagaa tacgtccggt gcagtacaga cctgagttca ggctggagag ggcagcggtc
     4081 agcaccacaa tgttcataat gctgccgata tatggcacac ccagtttaga gaaaaacgtc
     4141 acgaacggac tttgccccgc ctgatatgcg ctccacggca ataacataac cagcaacacc
     4201 acggagccga cgtaaaacag gccaatacgc caaatcacac tgttaatggc tttaggcacc
     4261 atggtctgcg gatctttaca ttctccggca gctgtaccca ccatttcaat ggaggcaaaa
     4321 gcaaacacta cgccctgaat caacaccagc gcaggcagca aaccgtgggg gaagaagccg
     4381 ccgttatcgg taattaaatg aaaaccagtg gtgttgccat ccagcggctg accactaccg
     4441 aggaacactg tacccacgac caaaaaggta acaatggcga gcactttaat aagcgcaaac
     4501 cagaactcca tctcggcaaa ccatttcaca ccgatcatat tcatggtgcc aacgatggtc
     4561 agcgcagcga gcgcaaagac ccactgcggc acgccgccaa acgcacccca gtaatgcata
     4621 tacagagcga cggcggtaat atcgacaatc cccgtcatcg cccagttgat gaagtacatc
     4681 cagcctgcaa cgtaagcggc tttctctcct aaaaactcac gggcataaga gacaaaactg
     4741 ccactggaag ggcggtgtaa caccagctca cccaatgcac gcagaataaa aaatgaaaac
     4801 aggccgcaga tcaaatagac cagtgccagt gctggccccg ccatttgcag tcgggctccc
     4861 gcgcctaaaa acaaacccgt accaatcgcg ccgccaatgg cgatcatctg tacctggcga
     4921 ttgcccatcg ctttgtgata cccctcttcg tgggcattaa gccagcggcg tttcgcggcg
     4981 tgttgatctg aagtgtcggt gtcgtgttta ctcattgctc tccctgattg ctttaatgaa
     5041 aaagtcatat aagttgccat gaacaatgtt tattcatcct ggcgatgata gttatcacca
     5101 ttattcttta tgggtataaa actatcggcg cagcatccta cccgttatcg ataaacgatg
     5161 caaaacatcc ccttacaatc ctgaagggga ataatacaac tgacgaaaaa atgacaaatc
     5221 cttttgctgg ttaacctgtg tactgtccta cacttaatct ttaaaagatt gtgaggggca
     5281 tatgattaag gtgtatggcg tacccggatg gggctctaca atcagtgagc tgatgctaac
     5341 actggctgat attccttatc agttcgttga tgtcagtggt tttgaccacg agggaacctc
     5401 acgcgagtta ttgaaaaccc tgaacccgtt gtgtcagatt ccgacactgg cgctggaaaa
     5461 tgacgagatt atgacggaga cagcggcgat tgcattgatg gttctcgatc gtcgcccgga
     5521 ccttgcgcca ccggttgggc gcgccgaacg tcagcagttt caacggctat tggtctggct
     5581 ggttgctaat gtctatccaa cattcacttt cgccgattac cctgagcgtt gggctcctga
     5641 cgcacccgaa cagttaaaga agaatgttat tgaatatcga aaatcgtttt atatctggtt
     5701 gaattcgtag ctcactgctg aaccttatgc gtttggcgat cagctaccct tatctctgcg
     5761 ttatgcgcat atggggaccg gggcatgaat ggtttcaaga taatgccgcg aatatcagtg
     5821 cgattgctga tgctgtatgt ccgataccaa cgttacaaga aatactaaaa aggaatgcca
     5881 ttatttaaca ataatgccgg agattaatgt taataaatca ctggcgtagt taac
//
LOCUS       DAOTRK010000207         5905 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.209, whole genome shotgun sequence.
ACCESSION   DAOTRK010000207 DAOTRK010000000
VERSION     DAOTRK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.209"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(28..681)
                     /gene="fdnI"
                     /locus_tag="QIE41_004355"
     CDS             complement(28..681)
                     /gene="fdnI"
                     /locus_tag="QIE41_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDS3533150.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(674..1558)
                     /gene="fdnH"
                     /locus_tag="QIE41_004356"
     CDS             complement(674..1558)
                     /gene="fdnH"
                     /locus_tag="QIE41_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDS3533151.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(1571..4618)
                     /gene="fdnG"
                     /locus_tag="QIE41_004357"
     CDS             complement(1571..4618)
                     /gene="fdnG"
                     /locus_tag="QIE41_004357"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4031..4033),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDS3533152.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWDEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4850..5731
                     /gene="yddG"
                     /locus_tag="QIE41_004358"
     CDS             4850..5731
                     /gene="yddG"
                     /locus_tag="QIE41_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDS3533153.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGCLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         1346 a         1620 c         1435 g         1504 t
ORIGIN      
        1 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
       61 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
      121 ctttccttcg atcatccctt taatcgatcc tttcacccag aatgccatat acatatggat
      181 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tataacggac
      241 aacctgtatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
      301 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
      361 accgacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
      421 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
      481 cgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc
      541 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
      601 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
      661 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
      721 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
      781 gcggtttcag cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
      841 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
      901 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
      961 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     1021 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     1081 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     1141 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     1201 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     1261 agccgtcttt acggatcagc cactccagct tgccgttctg ttcggtttcg ctaaagcgca
     1321 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
     1381 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacaaccga
     1441 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     1501 gcggcgtgat ggagttagtt gcggaccttt tgataatgtc ctgcgtttcc atagccatct
     1561 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     1621 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     1681 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     1741 gcggtttcag acggcgcgtt accaccgcca ccgcgcggat aaatccacgc ttgcttgaga
     1801 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc cagcgtttcg ctgatttcca
     1861 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     1921 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     1981 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacattcgga tgcagcgggt
     2041 tagtgcccag cggcgtttcg attggctcgt agtgttccgg gaacggacct tccgccattt
     2101 tgttgatggc aaacaggcgt cccatccctt ccggctgcat gataaacggt ccggttggtg
     2161 tacctggcgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     2221 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     2281 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     2341 cggacgggtc ggagttatcg cggttagcca tctggttgcc ctgctctgtc cagctaccgg
     2401 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     2461 gcaactgccc tttcttcgca atcagcacac cattagcatc atagagatct tccagcgcat
     2521 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     2581 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac agctcgcgca
     2641 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     2701 gacctttcca gtgccactgc aaccagcgac cggagttagc aatagaacca tcttcctcag
     2761 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgcagga tcgacatcgt
     2821 tcgactcgcc gtggttctgc cagaaggtag aagtttcagt caccagcgga tcgataacca
     2881 ccatatactt cagcttgctc aggcagctta ccactttgtt tttgtccggg aaggacgcaa
     2941 ccgggttaaa gccctggcag aaataaccgg tgactttgcc ttcatccatc atgttgaaat
     3001 acttgatgac gtcgtaggtc tggtcccact tgggcagcca gtcgtagccc cagttgttct
     3061 ctttctgcgc agcatcgccg tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     3121 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     3181 actgcaaatc aacctgtttt tctgacggca gcgtcagata acccggcagg ctggtagaga
     3241 gcaggcctaa gtcggtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     3301 caccggccat acccatgtta ccgagcagta actggatcat cgccatagta cggatgttct
     3361 gcgcacccac ggtgtgctgc gtccagccca gcgcgtaaag gaaggtggtt gtgcgatccg
     3421 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     3481 agatgttttc tactacgtcc ggcgtgtagc gggaaacgtg ctctttcagc aagttccaca
     3541 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     3601 agttccagga cgatttatcg tattgacgtt tttcagcgtc gtagccgctg aacagaccgt
     3661 cttcgaaagc aaaatcatca cgcaccagca gactggcgtt ggtgtaatgc ttaacgtatt
     3721 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt
     3781 ccgtaccgga acgaataggt gcgtaaatat ccgctacaga agcggtacgc gtaaaacggg
     3841 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     3901 cgacgggatg cgcttcagca gcgttaccgc ccatcaccat tacgacgtta gcgtttttga
     3961 tatccaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg
     4021 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     4081 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     4141 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     4201 cagctttcat cagcttcgca atacgggaga atgcttcatc ccagctaatg cgctgccatt
     4261 tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcgctg ttgacgtaat
     4321 ccagcaaacc ggcccctttc gggcataacg caccacggct taccggatga tccgggtcac
     4381 cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc atcacccagg ctatacatca
     4441 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     4501 atttatagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gctgctactg
     4561 ttgttcccgc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     4621 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     4681 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     4741 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     4801 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     4861 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     4921 tcgcggtgtc agtgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     4981 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagatcc cgaaaggcta
     5041 tttactcgcc gggtgtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg
     5101 atatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     5161 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     5221 aggattatta ttagccctgg ttggcgtctg ctgggtgtta ggtggtgaca atgggttaca
     5281 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     5341 tggtgcgttt atctgggcag cctactgcac agtaacgaat aaatacgcac gcggatttaa
     5401 cggaattacc gtttttgtcc tgctaacggg agcaagtctg tgggtttact attttctcac
     5461 gccacaacca gaaatggtat ttagcacgcc agtcatgatt aaactcatct ctgcggcatt
     5521 taccttagga tttgcttatg ctgcatggaa cgtcggtata ttacatggca acgtcaccat
     5581 tatggcggta ggttcgtatt ttacgcccgt cctttcctca gcgcttgcag ccgtgctgct
     5641 cagcgccccg ctgtcgttct cgttctggca aggcgcgcta atggtctgcg gcggttccct
     5701 gctctgctgg ctggcgacac gtcgtggcta aaataattgt cgggtctacg gcccggcagt
     5761 taatcagata aattccgaca atataaaaaa ataaatttca aataccagta gttcacctta
     5821 tatttcattt cgacttcgat cacatatcga taaataatat tatttcatct atttttctca
     5881 ttgtttttaa tcaccattct atttt
//
LOCUS       DAOTRK010000208         5895 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.210, whole genome shotgun sequence.
ACCESSION   DAOTRK010000208 DAOTRK010000000
VERSION     DAOTRK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.210"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..377
                     /locus_tag="QIE41_004359"
     CDS             <1..377
                     /locus_tag="QIE41_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533154.1"
                     /translation="ISNAEAIFYENQEEHIQRFLGRYTCRKPVLVYWESEQRWTSVNG
                     LEVMSMFDEYCHHIYLDDINKKIQAELPEDKTLGFKRQLQFFILGDEKIRIWVPQGEL
                     YFALMNVLQMFPLKKMVDDWTR"
     gene            497..874
                     /locus_tag="QIE41_004360"
     CDS             497..874
                     /locus_tag="QIE41_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533155.1"
                     /translation="MDECFINITPTSLSFKNAELSDLSYKNARLELTLICPAIKSEVK
                     LSFDWIHSFRVTDEGDLLGMSGIHGINQTGIYRVVNSAYFAWFTMQSCEIYKNNVIEH
                     YVIATSNDVIDVLSTVSPQIQYD"
     gene            990..1790
                     /locus_tag="QIE41_004361"
     CDS             990..1790
                     /locus_tag="QIE41_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDS3533156.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            1987..3425
                     /gene="aldA"
                     /locus_tag="QIE41_004362"
                     /pseudo
     CDS             1987..3425
                     /gene="aldA"
                     /locus_tag="QIE41_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(3467..4467)
                     /gene="gap"
                     /locus_tag="QIE41_004363"
                     /pseudo
     CDS             complement(3467..4467)
                     /gene="gap"
                     /locus_tag="QIE41_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            4656..5186
                     /gene="cybB"
                     /locus_tag="QIE41_004364"
     CDS             4656..5186
                     /gene="cybB"
                     /locus_tag="QIE41_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDS3533157.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            5431..5604
                     /locus_tag="QIE41_004365"
     CDS             5431..5604
                     /locus_tag="QIE41_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533158.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(5676..5825)
                     /gene="hokB"
                     /locus_tag="QIE41_004366"
     CDS             complement(5676..5825)
                     /gene="hokB"
                     /locus_tag="QIE41_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDS3533159.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1556 a         1302 c         1449 g         1588 t
ORIGIN      
        1 aaatctcgaa tgcagaggca atattttacg agaatcagga agaacacatt cagcgctttt
       61 taggcagata tacctgccgc aaaccggttc ttgtttattg ggaaagtgaa caaagatgga
      121 catctgtaaa cggtctggaa gtaatgagta tgtttgatga gtattgtcat cacatctacc
      181 ttgatgatat caataaaaaa atacaagctg aattacctga agataaaaca ttaggtttca
      241 aacgtcaatt gcaatttttc attttagggg atgagaagat tagaatatgg gtgccacagg
      301 gggaattata tttcgcatta atgaatgtat tgcagatgtt ccccctgaaa aaaatggttg
      361 atgactggac aaggtaattt caaatcacag taatgtccat cgattataca tggtacgtaa
      421 acagactcgc accgtttttg cagaaggtta atgtgtcgtc tatcgagagc agtctcatat
      481 atgaaggggt attaacatgg atgaatgttt tataaatatc actccgactt cattgtcttt
      541 taaaaatgcc gaactatcag atttaagcta taaaaatgcc aggcttgaac ttaccctcat
      601 atgtccggcg atcaagtcag aagttaagtt atcatttgac tggatacatt catttcgtgt
      661 tactgacgaa ggtgatttgt taggaatgtc aggaattcac ggtattaacc aaacggggat
      721 ctatagagta gtgaattccg cctactttgc atggtttact atgcaaagtt gtgaaattta
      781 taaaaataat gtgatcgagc attatgtcat tgccacgtca aatgatgtta ttgatgtttt
      841 atcaacagtt tctccgcaaa tacagtatga ctaaaaccag tgtctggttc gcgatggcga
      901 ggtagaaaaa gcatcatctt cctttcagag tgctccgctt ctcattatta tctcacgcag
      961 tattcttaag ggaacgataa ggaggaacca tgaacattac cccgtttccg acgctttcga
     1021 cggcaactat agatgccata aatgttatcg gacagtggct ggcgcaggat gatttctccg
     1081 gtgagatgcc gtatcaggcc gattgcgtga tccttgcagg caatgcggtt atgccgacta
     1141 tcgatgcggc atgtaagatt gcccgcgatc agcaaattcc tctactgatt agtggtggta
     1201 tcggtcactc gacaactttt ttgtatagcg ctatcgcaca gcatccgcac tacaacacta
     1261 tccgcaccac tggcagagca gaagccacca tcctggcgga tatcgctcat cagttctggc
     1321 atattccgca tgaaaaaatc tggattgaag accagtcaac aaactgcggt gaaaacgcac
     1381 gctttagcat cgcgctattg aatcaggccg tagaacgagt tcatacggct atcgttgttc
     1441 aggaccccac catgcagcgg cgcacgatgg cgacgttccg ccgtatgacc ggggacaatc
     1501 cagatgcacc tcgctggtta agttatcccg gattcgttcc tcagctagga aacaacgcag
     1561 acagtgtaat ctttgttaat ccgttgcaag gattatggcc ggttgagcgt tatctctcac
     1621 tactcactgg cgagctgccg cgtttacgcg atgatagcga tggctacggt ccccgcgggc
     1681 gagattttat cgttcacgtt gattttccgg cagaagtcat ccatgcatgg caaacgctga
     1741 aacacgatgc ggtgctcatc gaggcgatgg aaagtcgctc gttacgttaa aaattgcccg
     1801 tttgtgaacc acttgtttgc aaacgggcat aactcctgcc ttttatttct gcctcttatt
     1861 ccttttacac ttgtttttat gaagcccttc acagaattgt cctttcatga tgccgtctct
     1921 ctgatgattg atgttaatta acaatgtatt taccaaaaac aaacatataa atcacaggag
     1981 ccgctcatgt cagtacccgt tcaacatcct atgtatattg atggacagtt tgttacctgg
     2041 cgtggagacg catggattga tgtggtaaac cctgctacag aggctgtcat ttcccgcatt
     2101 cccgatggtc aggccgagga tgcccgtaag gcaatcgatg cagcagaacg tgcacaacca
     2161 gaatgggaag cgttgcctgc tattgaacgc gccagttggt tgcgcaaaat ctccgtcggg
     2221 atccgcgaac gcgccagtga aatcagtgcg ctgattgttg aagaaggggc aagatccagc
     2281 agctggctga agtcgaagtg gcttttactg cggactatat cgattacatg gcggagtggg
     2341 cacggcgtta cgagggcgag attattcaaa gcgatcgtcc aggagaaaat attcttctgt
     2401 ttaaacgtgc gcttggtgtg actaccggca ttctgccgtg gaacttcccg ttcttcctca
     2461 ttgcccgcaa aatggctccc gctcttttga ctggtaatac cattgtcatt aaacccagtg
     2521 aatttacgcc aaacaatgcg attgcattcg ccaaaatcgt cgatgaaata ggccttccgc
     2581 gcggcgtgtt taaccttgtt ctggggcgtg gtgaaaccgt tgggcaagaa ctggcgggta
     2641 acccaaaggt cgcaatggtc agtatgacag gcagcgtctc tgcaggtgaa aagatcatgg
     2701 cgactgcggc gaaaaacatc accaaagtgt gcctggaatt ggggggtaaa gcaccagcta
     2761 tcgtaatgga cgatgccgat cttgaactgg cagtcaaagc catcgttgat tcacgcgtca
     2821 ttaatagtgg gcaagtgtgt aactgtgcag aacgtgttta tgtacagaaa ggcatttatg
     2881 atcagttcgt caatcggctg ggtgaagcgt tgcaggcggt tcaatttggt aaccccgcag
     2941 aacgcaacga cattgcgatg gggccgttga ttaacgccgc ggcgctggaa agggtcgagc
     3001 aaaaagtggc gcgcgcagta gaagaagggg cgagagtggc gttgggtggc aaagcggtag
     3061 aggggaaagg atattattat ccgccgacat tgctgctgga tgttcgccag gaaatgtcga
     3121 ttatgcatga ggaaaccttt ggcccggttc tgccagtggt cgcatttgac acgctggaag
     3181 atgctatcgc aatggcgaat gacagtgatt acggcctgac ctcatcaatc tatacccaaa
     3241 atctgaacgt cgcgatgaaa gccattaaag ggctgaagtt tggtgaaact tacatcaacc
     3301 gtgaaaactt cgaagctatg caaggcttcc acgccggatg gcgtaaatcc ggtattggcg
     3361 gcgcagatgg taaacatggc ctgcatgaat atctgcagac ccaggtggtt tatttacagt
     3421 cttaatgagt gaaagaggcg gaggtttttt cctccgcctg tgcgcgtcag agtttagcga
     3481 atttttcgag ggtgcgaata agctgcgtga cgaagccata ttcgttatcg taccaggcga
     3541 ccgttttcac cagttgtaaa tcgcccacgg cggtaatttc ggtttgcgtg gcatcaaaca
     3601 ccgaaccgaa atggctgcca atgatatcgg aagagactat ttcttcatcg gtataaccaa
     3661 atgactcgtt attggtggtt gcttgtttaa gtgcgttatt cacctcttcg gcagtcactt
     3721 ttttccgaga atcgatacca gttcagtgac ggaacctgtt ttcaccggca cgcgttgcgc
     3781 atgacctttc agtttgccgc tcagttctgg gatcaccaga ccaatggctt ttgccgcccc
     3841 cgtagtgtgg ggaatgatat tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg
     3901 cgggccatcc accagtgact gagtgccggt ataggcatga atggtcgtca tcgtgccgac
     3961 ttctattccg aaactgtcgt gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca
     4021 tgacgccacg gaaacaatgg tgtcgttgcc atccagagtg tcgtcattga cgttataaac
     4081 gatagttttc atttcaccgg caggggcgga aatcaacacc ttcttcgcac cagcatcaag
     4141 atgcgcctgc gatttctcgg cggaggtata aaagccagta cattcgacaa taatttctgc
     4201 ccctttcgct ttccacggaa tatttttagc ctctttttcg gcgtaaaccg cgatactttt
     4261 cccatcaacg ataagtgaat cttccgtaaa atcaacgctc caggggaacg gtccatagtt
     4321 tgaatcatgt ttcagcaggt aggcgagaat ttttggggaa gtgagatcat taatagcgac
     4381 aatgtctatg ttgcttttga cttcaagtaa tcgacgcaac accagtcgac cgatacgacc
     4441 aaaaccgtta ataccaactt tactcatggt cttctcctgt caggaacgtt cggatgaaaa
     4501 ttgatccttt ccaggcttag accaggatgg cgggatgggc aatctacatt cttgccgtga
     4561 aacgtaacgt actgaaaacg ggtgaacaat atttaatgaa attttaagaa aagcccgtta
     4621 tgttaacgga aaattatgtt aaagcaggaa atgttatgga aaataaatac tcaaggttac
     4681 aaatcagcat tcactggctg gtctttttgc tggttattgc agcgtattgc gcaatggagt
     4741 ttcgggggtt cttcccacgt agcgatcggc cactcatcaa catgattcat gtttcctgtg
     4801 gcatatcaat tctcgtgctg atggtcgttc gtcttttgtt aaggctgaaa tacccaaccc
     4861 cgccgattat acctaagcca aaaccgatga tgacgggact ggcgcatttg ggacatttgg
     4921 tgatttatct cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccgag
     4981 gcaacccgtg gtttgcgttt ggtttgacga tgccttatgc atcagaggcc aatttcgaac
     5041 gggttgatag cttaaagtcg tggcatgaaa tgctggcgaa tctgggatat tttgtcatcg
     5101 ggttgcacgc tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac
     5161 gcatgatgcc gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg
     5221 acccgtggta caggcgaaga atacgggtct acatcggaag cgcctattat atttatttgt
     5281 atgacaaata aaaccataat ccttatccat acgtccatct ggcttatctt taatcaattc
     5341 gcccgatctt tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcga
     5401 acataagatt cgataaacca tggaggttat atgaaaaaac tggcacttat cttgtttatg
     5461 ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg
     5521 gggatctcac actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgca
     5581 tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct
     5641 agtcagatgc gcggggaaaa tccccgcgct tgcccttacc tggacgtgca ggccatgagc
     5701 gcagcaacct ccttatcacc gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg
     5761 agtgtgaatg tcagaatcgt aatgcagata atgagcagac acaccaccag agggttgcgc
     5821 ttcatagcct ttctccttgc ctgatggcgg gtaagaggct aacatctgaa ttgcttggtt
     5881 cattcgttgg cctcg
//
LOCUS       DAOTRK010000209         5872 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.211, whole genome shotgun sequence.
ACCESSION   DAOTRK010000209 DAOTRK010000000
VERSION     DAOTRK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.211"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(15..428)
                     /gene="hns"
                     /locus_tag="QIE41_004367"
     CDS             complement(15..428)
                     /gene="hns"
                     /locus_tag="QIE41_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDS3533160.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1032..1649
                     /gene="tdk"
                     /locus_tag="QIE41_004368"
     CDS             1032..1649
                     /gene="tdk"
                     /locus_tag="QIE41_004368"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDS3533161.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(1952..4627)
                     /gene="adhE"
                     /locus_tag="QIE41_004369"
                     /gene_synonym="adhC"
     CDS             complement(1952..4627)
                     /gene="adhE"
                     /locus_tag="QIE41_004369"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDS3533162.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            5104..5751
                     /gene="ychE"
                     /locus_tag="QIE41_004370"
     CDS             5104..5751
                     /gene="ychE"
                     /locus_tag="QIE41_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDS3533163.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         1476 a         1312 c         1416 g         1668 t
ORIGIN      
        1 caatctacaa aagattattg cttgatcagg aaatcgtcga gggatttacc ttgctcatcc
       61 attgcttttt tgattacagc tggagtacgg ccctggccag tccaggtttt agtttcgccg
      121 ttttcgtcaa cgtagctata ttttgccgga cgctgagcac gtttagcttt ggtgccagat
      181 ttaacggcag caaggctatt cagcagttcg ttcgggtcaa taccgtcagc gatcagcatt
      241 tcgcgatatt gctgcagttt acgagtgcgc tcttcaactt cagcagcagc cgcgctttct
      301 tcttcgcgac gttcgttaac aacaacttct aatttttcca gcatttcttc cagcgtttca
      361 agtgtacatt ctcttgcctg cgcacgaaga gtacggatgt tgttcagaat tttaagtgct
      421 tcgctcattg tagtaatctc aaacttatat tggggtggtt tgttgaggta ataatagagc
      481 cttaaattca gttgtgcaat agccaggaat gtaaggaatt caaaattgtt ctttattttg
      541 tgccgccaat aaatatcttt tcataaaatt agccagaaaa gacgcggcat atagccctat
      601 ttgcgccgat gatttcgcag cacgtgaggt taaaacttcc tgattcatgt cacattttat
      661 ggggagatta tcgtaggctg acgacctttc agtcttctgt attagtcgtg tttacgagaa
      721 ttccctatta agcgaatgat gaaaagtaga acagtcgcaa taagagcagg gacttagtat
      781 tgcactatct ccaggaggtc aacagagggc tattacttgc gcaacaggtt aaagattgtg
      841 aatagttacc agcagtcatt tacccgctta taacaagcga ggcagttgta atgatagctc
      901 agaaggatta tgcaaggctt cgtaagggag aacgcatata ccacttctgt gcatactgtt
      961 gagctgaaaa actgacgaat tatgataaac tccagccaac tttaattcat atcattgagg
     1021 gcctgtggct gatggcacag ctatatttct actattccgc aatgaatgcg ggtaagtcta
     1081 cagcattgtt gcaatcttca tacaattacc aggaacgcgg catgcgcact gtcgtatata
     1141 cggcagaaat tgatgatcgc tttggtgccg ggaaagtcag ctcgcgtata ggtttgtcat
     1201 cgcctgcaaa attatttaac caaaattcat cattatttga tgagattcgt gcggaacatg
     1261 aacagcaggc aattcattgc gtactggttg atgaatgcca gtttttaacc agacaacaag
     1321 tatatgaatt atcggaggtt gtcgatcaac tcgatatacc cgtactttgt tatggtttac
     1381 gtaccgattt tcgaggtgag ttatttattg gcagccaata cttactggca tggtccgaca
     1441 aactggttga attaaaaacc atctgttttt gtggccgcaa agcaagcatg gtgctgcgtc
     1501 ttgatcaagc aggcagacct tataacgaag gtgagcaggt ggttattggt ggtaatgaac
     1561 gctacgtttc tgtatgccgt aaacactata aagaagcgtt agaagtcggc tcattaacgg
     1621 ctattcagga aaggcatcgc cacgattaat aagaatttct ttactgacag ggtgagcagg
     1681 gcgcttttat cctgtcagtt cgttttactc acttcatccg gtgtatatac ccttctcgac
     1741 agttctttaa cgccactata cccctcacag agcgctttta cagtgacatt gacttgtgac
     1801 ctgtaaagta caatatcgct gtgtgtaggc gttatacatc gccgtaaata tgatgaaggc
     1861 aaatgctgtc ggtttatggg aaagttgctt tgggtaaaac aaaaaaacgg ccccagaagg
     1921 gaccgtttat gttgccagac agcgctactg attaagcgga tttcttcgct tttttctcag
     1981 ctttagccgg agcggcttct tttttcgctg cagtttcacc ttctacataa tcacgaccgt
     2041 agtaggtatc cagcagaatc tgtttcagct cggagatcag tgggtaacgc gggttagcgc
     2101 cggtgcactg gtcatcgaat gcatcttcag acagtttatc cacattcgcc aggaagtctg
     2161 cttcctgaac gccagcttca cggatagatt tcggaatacc cagttcagct ttcagcgttt
     2221 ccagccatgc cagcagtttc tcgatcttag cagcagtacg gtcgcccggt gcgctcagac
     2281 ccaggtggtc agcaatttca gcataacgac ggcgagcttg cggacggtca tactggctga
     2341 atgcagtctg cttggtcggg ttgtcgttcg cgttgtagcg aataacgtta caaatcagca
     2401 gggcgtttgc cagtccgtgc ggaatatgga actgggaacc cagtttgtgc gccattgagt
     2461 gacatacacc caggaaggcg ttcgcaaacg cgatacccgc gatagttgct gcactgtgaa
     2521 cacgttcacg cgctaccgga tttttagacc cttcgtggta ggacgctggc agatattctt
     2581 tcagcagttt cagtgcctgc agagcctgac catcagagaa ctcagatgcc agtacagaaa
     2641 cataagcttc catggcgtga gttactgcgt ccagaccacc gaaagcacac agggacttcg
     2701 gcatgtccat aaccaggttg gcatcgacaa tcgccatatc cggggtcagc gcatagtctg
     2761 ccagcggata tttctgacca gtagcgtcgt cagttacaac cgcaaacgga gtgacttcag
     2821 aacctgtacc agaagtggtg gtgacagcga tcattttcgc tttcacgccc attttcggga
     2881 acttgtagat acgtttacgg atatccataa agcgcagcgc cagttcttcg aagtgagttt
     2941 ccggatgttc gtacatgacc cacatgatct tcgcggcgtc catcggggaa ccaccaccca
     3001 gcgcgataat cacgtctggt ttgaaggagt ttgccagttc tgcaccttta cgaacgatgc
     3061 tcagggtcgg gtcagcttct acttcgaaga agacttcagt ttcaacgcct gctgctttca
     3121 gtacggaagt gatctgatca gcataaccat tgttgaacag gaagcggtca gtcacgatga
     3181 gcgcacgttt gtggccatca gtaatcactt catccagcgc gattggcagg gagccacggc
     3241 ggaagtagat agatttcgga agtttgtgcc acaacatgtt ttcagctcgc ttagcaacgg
     3301 ttttcttgtt gatcaggtgt ttcggaccaa cgttttcaga gatggagtta ccaccccagg
     3361 aaccacaacc cagagtcagg gaaggtgcga gtttgaagtt atacaggtca ccgataccac
     3421 cctgagaagc cggggtgtta atcaggatac gagccgtttt cattttctga ccgaagtaag
     3481 aaacgcgagc cggttggtta tcctggtcag tgtacaggca agaggtatga ccgataccgc
     3541 ccatagctac cagtttctct gctttttcta ccgcgtcttc gaaatcttta gcgcggtaca
     3601 ttgccagagt cggggacagt ttttcatgtg cgaacggttc gctttcatca acaacggtca
     3661 cttcaccgat cagaatcttg gtgttttctg gtacagagaa gcctgccagt tcagcaattt
     3721 tataggctgg ctgaccaacg atagccgcgt tcagcgcacc gtttttcagg ataacgtcct
     3781 gaacagcttt cagctcttta ccctgcaaca gatagccgcc atgggttgcg aaacgttcac
     3841 gtactgcgtc ataaacagag tcaacaacaa caacagactg ttcagaagca cagattacgc
     3901 cgttgtcgaa ggttttggac atcagcacag atgcaactgc acgtttgata tcagcagttt
     3961 catcgataac aacaggcgtg ttacccgcgc ctacaccgat agctggttta ccggagctgt
     4021 atgcggcttt aaccatgccc ggaccaccag tcgcgaggat caggttgatg tctgggtggt
     4081 gcatcagtgc gttagacagt tcaaccgaag gttgatcgat ccagccgatc agatctttcg
     4141 gagcaccggc agcgatagca gcctgcagaa cgatatcagc tgctttgttg gttgcatctt
     4201 ttgcacgtgg gtgcggggag aagataatgg cgttacgggt cttcaggctg atcagcgatt
     4261 tgaagatagc agttgaagtc gggttagtgg tcggaacgat accgcaaata ataccgattg
     4321 gttcagcgat agtgatggta ccaaaagtgt cgtcttcaga cagaacacca caggtttttt
     4381 catctttata ggcgttgtag atatattcag aagcaaagtg gtttttgatc actttatctt
     4441 cgacgatacc catgccggat tcggcaacgg ccattttcgc gagtgggatt cgagcatctg
     4501 cggcagccag agcggcggcg cggaagattt tgtctacttg ctcttgagtg aaactggcat
     4561 attcacgctg ggcttttttt acacgctcta cgagtgcgtt aagttcagcg acattagtaa
     4621 cagccataat gctctcctga taatgttaaa cttttttagt aaatcatctg ctcgaatacg
     4681 agagtatagt cagtgcggtg atgatttgct taaccgatga aaatcaaaag cttactcgcg
     4741 ctcacactca ctgtgattta ctaaaagagt ttaaacatta gagttattat ctctaatgcg
     4801 tcacttccag gtggcgtaag caagattact cacttctggg tactgattac gtgatccaaa
     4861 tcaaattttt gcaaagctga cacctttcag catcgctttt cgccattata gctaacagtt
     4921 aataaattgt agtatgattt gttggctaca ttatcatgtt ttgcacaacc agataacaat
     4981 aacgaatgat agcaatttta agtagttagg aggtgaaaaa tgctgtcaaa aggcgtattg
     5041 tcagcgcgtc tttttaacct tatttatggc taacattatc cggcttttgc ttcggagcta
     5101 accgtgattc agaccctttt tgattttccc gtttacttca aatttttcat cgggttattt
     5161 gcgctggtca acccggtagg gattattccc gtctttatca gcatgaccag ttatcagaca
     5221 gcggcagcgc gaaacaaaac taaccttaca gccaacctgt ctgtggccat tatcttgtgg
     5281 atctcgcttt ttctcggcga cacgattctg caactttttg gtatatcaat tgattcgttc
     5341 cgtatcgccg ggggcatcct ggtggtgacg atagcgatgt cgatgatcag cggcaagctt
     5401 ggcgaggata aacagaacaa gcaagaaaaa tcagaaaccg cggtacgtga aagcattggt
     5461 gtggtgccac tggcgttgcc gttgatggcg gggccagggg cgatcagttc taccatcgtc
     5521 tggggtacgc gttatcacag cattagctat ctgtttggtt tctttgtggc tattgcattg
     5581 ttcgctttat gttgctgggg attgttccgc atggcaccgt ggctggtacg ggttttacgc
     5641 caaaccggca tcaacgtgat cacgcgtatt atggggctat tgctgatggc attggggatt
     5701 gaatttatcg ttactggtat taaggggatt ttccccggcc tgcttaatta attcctttca
     5761 aatgaaacgg agctgtcatg ctccgtttac ttcgtcatta tttttacttt gttcccgcgc
     5821 agttatcaaa agcaaaagga atgggtgaaa atattcttct caaattacag tt
//
LOCUS       DAOTRK010000210         5845 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.212, whole genome shotgun sequence.
ACCESSION   DAOTRK010000210 DAOTRK010000000
VERSION     DAOTRK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.212"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            80..1510
                     /locus_tag="QIE41_004371"
     CDS             80..1510
                     /locus_tag="QIE41_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HDS3533164.1"
                     /translation="MIRKSATGVIVAFAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQITFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGSSFSTTLDVAP
                     AEYGKFSFGKGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDTFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1551..2483)
                     /locus_tag="QIE41_004372"
     CDS             complement(1551..2483)
                     /locus_tag="QIE41_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDS3533165.1"
                     /translation="MANFLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHLQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2847..5633
                     /gene="polA"
                     /locus_tag="QIE41_004373"
     CDS             2847..5633
                     /gene="polA"
                     /locus_tag="QIE41_004373"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDS3533166.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         1715 a         1302 c         1421 g         1407 t
ORIGIN      
        1 ataatgcgtc ctgccgctgg acattatttc agcataagtg gatattcaaa gttttgctgt
       61 tttatcaggg aatatttata tgatacgtaa gtcagctaca ggtgttattg ttgcgtttgc
      121 cgtaatctgg ggtggtggca catggtacac aggtacgcaa attcagcctg gtgtcgaaaa
      181 gttcattaaa gattttaacg atgctaaaaa gaaaggtgaa catgcctacg atatgacgtt
      241 aagttataaa aactttgaca aaggcttttt taattctcat tttcaaatgc aaataacatt
      301 tgataacggt gcacccgatc tcaatatcaa gccaggccag aaagttgcat ttgatgtgga
      361 tgttgagcac ggtccgttgc ccatcacaat gttaatgcgt ggtaatgtca tccccgcact
      421 ggcagcggca aaagtgaact tagtgaataa tgaactgaca caaccgctat ttatcgccgc
      481 gaaaaataaa tcgcccgtgg aagcgacatt gaggttcgcg tttggtagct cattctccac
      541 gacattagat gttgcccctg cagagtatgg aaagttttcc tttggtaagg gccagtttac
      601 ttttaatggt gatggtagtt cattgtctaa cctggatatt gaaggcaaag tcgaagatat
      661 tgttctgcaa ttatcaccaa tgaacaaagt aacggcgaaa agctttacca ttgattctct
      721 ggcgcgatta gaagaaaaga aatttccggt tggtgaaagc gagtcgaaat ttaatcagat
      781 taacattatc aatcacgggg aagacgttgc ccaaatcgat actttcgttg caaaaaccag
      841 gctggatcgc gttaaagaca aagattatat caatgtcaat ctgacctacg aacttgataa
      901 gttaacaaaa gggaatcagc aactcggtag tggtgagtgg tcattgattg ctgaatctat
      961 tgatccttca gcggtgcgcc aatttatcat ccagtataac attgcgatgc agaagcagct
     1021 tgctgcacac cctgagttag caaacgatga agttgctctg caagaagtga atgctgcatt
     1081 gttcaaagag tatttaccgt tattacaaaa aagtgagccg accattaaac aaccggtaag
     1141 atggaagaac gcactcggcg aactaaatgc caatctggat atcagtattg ccgacccagc
     1201 caaatcctca tcatccacaa acaaagatat taaatcgctc aattttgatg tgaagttacc
     1261 gcttaatgtc gtcacagaaa ccgcaaaaca gcttaattta tctgaaggaa tggatgctga
     1321 aaaagcgcaa aagcgggctg ataaacaaat cagcgggatg atgacattag gtcagatgtt
     1381 tcagttaatc acgattgaca acaataccgc ctcgctgcaa ctgcgttata caccgggtaa
     1441 agttgttttt aacggacagg aaatgagcga agaagaattt atgtctcgtg ccggacgttt
     1501 tgttcattaa aatactcact cgcctctgct cattcagagg cgagtaaaac ttacttattc
     1561 tgacgctgtg atgacatcaa cgacgtcaga agtcggtctt tttcctgcca cagtgagttc
     1621 agccattgct gaaaatggcg cttaaagctt ttgtcattga tgtagtcgcc atgtaactca
     1681 tcggaaatag gctgcaaatc cacatgtacg acaattcgcg tcagtttacc gcttaacata
     1741 tcgaagaacg gctgacggtt attgtccgga taacacagcg taacattaag cagtttatcg
     1801 aattgtttac ccagtacatt tagcgccatc gcaatgcctg ccgcctttgg tggcaacaag
     1861 ttttgaaaag tggagtgggt ttgcagatgt ttttcttgcg tgaaacggga gccttcaacg
     1921 aaattgacaa tggtggtggg atgcaggcga aacttttcac aagaacgccg agtggtttcg
     1981 acatctttac cacgtcgctc cggatgacgt aataaataag cgcgggaata acgcttcata
     2041 aacggcatat ccagcgccca gcacgccagg ccaagaaaag gcacccaggc cagctgctgc
     2101 ttgaggaaat atttattcat cggaatgtgc ttacgaaaca gcacgcacag tacgacaata
     2161 tctgcccaac tacggtggtt acaaataagc agataccagt tcttcttact taacccttcc
     2221 agcccgtgaa cttcccattg caggtgtggg ttcagatgca gtaataccgc cagaccttcg
     2281 caccagcaat acatcataaa atcacaaaaa cgcgaaacct ttcgccaaat gactggcaca
     2341 ggaagcaata gttttacaat cccggcaatg atgatcggga cggagcaaaa aatagtgacc
     2401 aaaatggtca atacgatact cagcaaaagg gttatcgcag cgagtattct cgtcataatg
     2461 aatttattca aaaagttagc catagacagt gcgctaaaga agcgcaaggc gcagatttta
     2521 gcagaaaacg acccaaataa cggatgatcc ttaaggagaa aaataattca tatctatcca
     2581 cattagaaaa aatcccatta tctcaattat tagggatggt cttattttta acttcatgaa
     2641 aaacaaagac aaacatcatg ctgtaaaaag catgataata aattaaaagc gatgtaaata
     2701 atttatgcac aaagttatcc acatgacgat ttgcgagcga tccagaagat ctacaaaaga
     2761 ttttcacgaa aagcggtgaa aaactcatgt tttcatcctg tctgtggcat cctttaccca
     2821 taatctgata aacaggcacg gacattatgg ttcagatccc ccaaaatcca cttatccttg
     2881 tagatggttc atcttatctt tatcgcgcat atcacgcgtt tcccccgctg actaacagcg
     2941 caggcgagcc gaccggtgcg atgtatggtg tcctcaacat gctgcgcagt ctgatcatgc
     3001 aatataaacc gacgcatgca gcggtggtct ttgacgccaa gggaaaaacc tttcgtgatg
     3061 aactgtttga acattacaaa tcacatcgcc cgccaatgcc ggacgatctg cgtgcacaaa
     3121 tcgaaccttt gcacgcgatg gttaaagcga tgggactgcc gctgctggcg gtttctggcg
     3181 tagaagcgga cgacgttatc ggtactctgg cgcgcgaagc cgaaaaagcc gggcgtccgg
     3241 tgctgatcag cactggcgat aaagatatgg cgcagctggt gacgccaaat attacgctta
     3301 tcaacactat gacgaatacc atcctcggac cggaagaggt ggtgaataag tacggcgtgc
     3361 cgccagaatt gatcatcgac ttcctggcgc tgatgggtga ctcctctgat aacattccag
     3421 gcgtaccggg cgtcggtgaa aaaaccgcac aggcattgct gcaaggtctt ggcggactgg
     3481 atacgctgta tgccgagcca gaaaaaattg ccgggttgag cttccgtggc gcgaaaacaa
     3541 tggcagcgaa gctcgagcaa aacaaagaag ttgcttatct ctcataccag ctggcgacga
     3601 ttaaaaccga cgttgaactg gagctgacct gcgaacaact ggaagtgcag caaccggcag
     3661 cggaagagtt gttggggctg ttcaaaaagt atgagttcaa acgctggact gctgatgtcg
     3721 aagcgggcaa atggttacag gccaaagggg caaaaccagc cgcgaagcca caggaaacca
     3781 gtgttgcaga cgaagcacca gaagtgacgg caacggtgat ttcttatgac aactacgtca
     3841 ccatccttga tgaagaaaca ctgaaagcgt ggattgcgaa gctggaaaaa gcgccggtat
     3901 ttgcatttga taccgaaacc gacagccttg ataacatctc tgctaacctg gtcgggcttt
     3961 cttttgctat cgagccaggc gtagcggcat atattccggt tgctcatgat tatcttgatg
     4021 cgcccgatca aatctctcgc gagcgtgcac tcgagttgct aaaaccgctg ctggaagatg
     4081 aaaaggcgct gaaggttggg caaaacctga aatacgatcg cggtattctg gcgaactacg
     4141 gcattgaact gcgggggatt gcatttgata ccatgctgga gtcctacatt ctcaacagcg
     4201 ttgccggtcg ccacgatatg gacagcctcg cggaacgttg gttgaagcat aaaaccatca
     4261 cttttgaaga gattgctggt aaaggtaaaa atcaactgac ctttaaccag attgccctcg
     4321 aagaggccgg acgttacgcc gccgaagatg cagatgtcac cttgcagctg catctgaaaa
     4381 tgtggccgga tctgcaaaaa cacaaagggc cgttgaacgt cttcgagaat atcgaaatgc
     4441 cgctagtgcc ggtgctttca cgcattgaac gtaacggtgt gaagatcgat ccgaaagtgc
     4501 tgcacaatca ttctgaagag ctcactcttc gtctggctga gctggaaaag aaagcgcatg
     4561 aaattgcagg tgaagaattt aacctttcct ccaccaagca gttgcaaacc attctgtttg
     4621 aaaaacaggg aattaaaccg ctgaagaaaa cgccgggtgg cgcgccgtca acgtcggaag
     4681 aggtactgga agaactggcg ctggactatc cgttgccaaa agtgattctg gagtatcgtg
     4741 gtctggcgaa gctgaaatcg acctacaccg acaagctgcc gctgatgatc aacccgaaaa
     4801 ccgggcgtgt gcatacctct tatcaccagg cagtaactgc aacgggacgt ttatcgtcaa
     4861 ccgatcctaa cctgcaaaac attccggtgc gtaacgaaga aggtcgtcgt atccgccagg
     4921 cgtttattgc gccagaggat tatgtgattg tctcggcgga ctactcgcag attgaactgc
     4981 gcattatggc gcatctctcg cgtgacaaag gcttgctgac cgcattcgcg gaaggaaaag
     5041 atatccaccg ggcaacggcg gcagaagtgt ttggtttgcc actggaaacc gtaaccagcg
     5101 agcaacgccg tagcgcgaaa gcgatcaact ttggtctgat ttatggcatg agtgctttcg
     5161 gtctggcgcg gcaattgaac attccacgta aagaagcgca gaagtacatg gacctttact
     5221 tcgaacgcta ccctggcgtg ctggagtata tggaacgcac ccgtgctcag gcgaaagagc
     5281 agggctacgt tgaaacgctg gacggacgcc gtctgtatct gccggatatc aaatccagca
     5341 atggggctcg tcgtgcagcg gctgaacgtg cagccattaa cgcgccaatg cagggaaccg
     5401 ccgccgacat tatcaaacgg gcgatgattg ccgttgatgc gtggctacag gctgagcaac
     5461 cgcgtgtacg tatgatcatg caggtacacg atgaactggt atttgaagtt cataaagatg
     5521 atgtcgatgc cgtcgcgaag cagattcatc aactgatgga aaactgtacc cgtctggatg
     5581 tgccgttgct ggtggaagtg gggagtggcg aaaactggga tcaggcgcac taagattcgc
     5641 ctgaatatgc ccttttttcg taagtaagca acataagctg tcacgttttg tgatggctat
     5701 tagaaattcc tatgcaacaa ctgaaaaaaa attacaaaaa atgctttctg aactgaacaa
     5761 aaaagagtaa agttagtcgc gtagggtaca gaggtaagat gttctatctt tcagaccttt
     5821 tacttcacgt aatcggattt ggctg
//
LOCUS       DAOTRK010000211         5804 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.213, whole genome shotgun sequence.
ACCESSION   DAOTRK010000211 DAOTRK010000000
VERSION     DAOTRK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.213"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..593)
                     /locus_tag="QIE41_004374"
     CDS             complement(<1..593)
                     /locus_tag="QIE41_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDS3533167.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="QIE41_004375"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="QIE41_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDS3533168.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="QIE41_004376"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="QIE41_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDS3533169.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="QIE41_004377"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="QIE41_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDS3533170.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="QIE41_004378"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="QIE41_004378"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDS3533171.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="QIE41_004379"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="QIE41_004379"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDS3533172.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPLTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="QIE41_004380"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="QIE41_004380"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDS3533173.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSCVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQSGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /locus_tag="QIE41_004381"
     CDS             complement(4982..5704)
                     /locus_tag="QIE41_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS3533174.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
BASE COUNT         1433 a         1500 c         1417 g         1454 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaaatc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcatattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggagcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtatct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatca attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttgatttc
     2041 tgcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcgtga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag tcaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataacagca gttcgtcgcc ttcactgagc agacgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aatctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtcagcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc aatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacacc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggtgcagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccga ctgaagattt
     4681 gacgaagcca gccccagcct tcctgcttca gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagccgcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgatagaaaa agacgcagct taactcatgg ccttcggcta tcagtgcttg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcgtcttta atatcgaaga
     5101 tccctttctc gtagagattt agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcagacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttc
//
LOCUS       DAOTRK010000212         5771 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.214, whole genome shotgun sequence.
ACCESSION   DAOTRK010000212 DAOTRK010000000
VERSION     DAOTRK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.214"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..520)
                     /gene="nac"
                     /locus_tag="QIE41_004382"
     CDS             complement(<1..520)
                     /gene="nac"
                     /locus_tag="QIE41_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDS3533175.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINQQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQD"
     gene            846..921
                     /locus_tag="QIE41_004383"
     tRNA            846..921
                     /locus_tag="QIE41_004383"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:879..881,aa:Asn,seq:gtt)
     gene            complement(977..1912)
                     /gene="ldtA"
                     /locus_tag="QIE41_004384"
     CDS             complement(977..1912)
                     /gene="ldtA"
                     /locus_tag="QIE41_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDS3533176.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(1974..3053)
                     /gene="cobT"
                     /locus_tag="QIE41_004385"
     CDS             complement(1974..3053)
                     /gene="cobT"
                     /locus_tag="QIE41_004385"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDS3533177.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(3065..3808)
                     /gene="cobS"
                     /locus_tag="QIE41_004386"
     CDS             complement(3065..3808)
                     /gene="cobS"
                     /locus_tag="QIE41_004386"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDS3533178.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAISRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(3805..4350)
                     /gene="cobU"
                     /locus_tag="QIE41_004387"
     CDS             complement(3805..4350)
                     /gene="cobU"
                     /locus_tag="QIE41_004387"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDS3533179.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         1548 a         1534 c         1226 g         1463 t
ORIGIN      
        1 aatcttgagt tcctaccagg aaaagatctt ctttcagcaa agcctgactg gatacaccag
       61 ccacagggga atgctcataa atcaccgcca tatcgagttg ttgatttatc agtttttcgt
      121 taagcactgc accgctattt tcatgaagat agataacgac ctccgggaat tcagcgcgaa
      181 ccgcctgtaa taagggcatg gtgatggatg acgcagcggt tcctggcgca aagccaatcg
      241 agacttgccc cgataatgcc tgaccaacgt tatgcaccgc cagttgggcc tgctcacact
      301 gacgtaaaat ggcccgcgca tgggtataga gaatttttcc ggcgtctgtt ggcgtaacgc
      361 cccgttttgt gcgaatcaaa agttgttgat ttaactcacc ttcgagtgtg gcaacctgct
      421 ggctgagcgc tggttgtgcg atatgcaata cttcagcagc ctgagtcagg ctaccaatat
      481 ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat gttgcctccg gtttttgaga
      541 atcggcccaa gtgccgccat tacttacaac cagattgcaa gatgcttgcc agttttattt
      601 tggcgttgat gtacaagcta accaactgtc aaataagaga ttatgataga ttcgtcattg
      661 gctcttttca ccagcagtcg cgttcccctg ccctataaaa ggagggtatg caccacgatg
      721 gttcattacc aataagattg aaatctcatc actttgttga aattgacagc aaacaaacaa
      781 aaaaatgcat ttcacccttt gacatcacca tgcactgcca ttaatatgcg ccccgttcac
      841 acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
      901 cgagtccagt cagaggagcc aaattcaaaa aagcctgctt tctagcaggc tttttgcttt
      961 ctaattacca acgctcttaa aacatctgtc ttgaaccaga actaatttgc acaggcattc
     1021 ccgatcgacg ttgcaacgca gcatttgcgc gattcacatc aacttcttgc ccgttgataa
     1081 acgcccgcaa agatggggtt accggcaatg gcacttttcg gtcagactca tattcagcac
     1141 gattgcgcga cagcggctca tgaacttcca gccactttga accatccggt tccgttgtgt
     1201 atttcactgg ctgatcaata atttgcacac gagtacctac aggaacatta tcaaacagat
     1261 atttgatatc gtcattgcgc agacgaatac agccctgact tacccggagc ccaataccaa
     1321 aattggcatt ggtaccgtgg atggcatata gcctgccaat ataaatcgcg tacagcccca
     1381 tgggattatc gggccccgca ggaacaaatg cgggcaaact ctcccctcgt ttcgcatatt
     1441 cgcgccgggt gttcggcgtt ggcgtccagg ttggtgcttc ttgtttacgt tcaacggtag
     1501 tcacccagtt acgcggggtt tctcgcccag cctggccgat accaatagga aagacttcca
     1561 cagtattact gtctggtggg tagtaataaa gacgcatctc agcgacgtta accacaatcc
     1621 ctttacgaac agtggcgggc aaaatcagtt gctgcggaat ggtgagttgc gaacctgact
     1681 tcggtaagaa gacatcagcg cccggattcg cttccagcat gttacttaac ccttgcccgt
     1741 attgtgcggc aaaagtctcc agcggctggg tattgtgatc aggaacagtt acagtaagcg
     1801 actgccccac taaacggcta ccctctggag gtaatggata agttaccgcc aggctagtat
     1861 ggctggcaaa aagcagagca aatgagcaaa gaatatttac acgacgcatc atgtcccttt
     1921 cctaagtcgc gaaagctatc ggttaagtat agcttttatc agacttttcg tttttaactg
     1981 ttcaaatcag aagtcgtatt ccccggtaga acaattttac tggcagcaag ttcgcccatg
     2041 ttgttgtata tcgcacaagc agcttcgatg atgggcatcg ccagagctgc gccactcccc
     2101 tcacctaaac gcatatccat attgagaaaa ggctccagcc ccaaatgcga cagcgctata
     2161 cgcgcccctt tttctgccga caggtgagaa ggaatgagat acggtttgat tgcaggagac
     2221 atctggcagg ctgcgagcgc agcagcataa gaaagaaatc catccagcaa cacgggtaaa
     2281 ccgcaggaag cagcgcctaa catcacgcca gccattccga ccaaatcaaa tccacccact
     2341 tttgccagaa cattaacgcc atcctgcgga tttggctgat tcaacgtaat cgcccggcgc
     2401 acaacatcaa ttttattagc cagtttatct gtcggcaggt ttgcgccaat cccaaccact
     2461 tcttcaggat cccggccagt gattgtgctg actattgctg ctgccggtgt cgtgtttgcc
     2521 atccccagtt cacctacacc aaacagcgtg acaccgtttt ttgccagctc ccgcgtataa
     2581 catatgacgt ccaaaagtaa cttttcagcc tgacggcgac tcattgccgg agctgaagca
     2641 atattgccgc tacctcgtgc gacacgcatg ttgataagcc cggggatagg ctcagcagta
     2701 tcaataccaa catcaattac gtggacgtta gcgcccgctt gtgctgccag cacacacacg
     2761 ccggttgttc cgcgggtcat attttcagcc tgaatggctg tcacttcttt tggggaaata
     2821 gcgacccctt cctcccagac gccgtgatcg gcacacataa ccagtaccgc ttttttgccc
     2881 acatgcggta tgccattcaa ccccggcatt cccgccagtt gtacgccaag cgcctccagc
     2941 cttcccaggc taccaacagg tttgagtaac ccgtcaatat gccgttgtgc acgcgacata
     3001 gcggcaggat cgatggcagg gatcgtattc agtaaattgg caagtgtttg catgtcacgt
     3061 ctctttatag cagtgccagc aggaagacca gttcaccaag ctctatggct gctcccagcg
     3121 tatcgccggt ttgcccgccc agcgtacgtt tgagaagctg accgaggatg aaaatcgcca
     3181 ccatcgtcac caccatagca gccacaccat gcatacccgg caataatacg gcagcaaaaa
     3241 tagcggctaa gccgagtgtg acgcaggttt gtcgcccatc aattttgcca atgaacacat
     3301 tgccaagccc ttcttcccgg gcataacgat gacgatacat caataatgcg gcagtcccgc
     3361 ggctgatcgc acatgctgcc gccagcgatg caaggatcgg ttcgccacgc agagccaact
     3421 cgcttaacac caaaatcttt gccagtacaa caaaaatcaa tgccagaccg ccgtgggtac
     3481 ctaaacggct atcacgcatg atttccaaca tgcgatcgcg gctacgtgca gaaaatacgc
     3541 catcacaggt atcagcgagg ccgtccagat ggaatccccc ggtcatcagc accagcacaa
     3601 gtacgctaaa cagtgccgcc agtggtgcgc cacaccatgc ctgcagcacc atgaagacca
     3661 gcccgctaat cgcgccaagc aataatccaa tcaaaggaaa agtaataata ccgcgagaat
     3721 aatgctcgaa atccagtccc tgggaccagc gacgcggtac aggcagacgc gtaataaatg
     3781 agagcatcgc ccaaaataat ttactcattt gatttttact ccaatacccg aaaccaccag
     3841 ccatacttca tttgccgcag cggccaactg ctgatttacc cgcccggcaa tatcacgaaa
     3901 atgtcgtgcc agacgactct ccggcacaat ccccattccc acttcgttag tcactaatac
     3961 aacctttgcg gggcaacgtt ggcaggcagc aatcaacgac tgaatctcag cattaatcgc
     4021 ctgttccatc gcctgataat cccattcatc agggtcttta tcgccgccat aatcaaacaa
     4081 cagattagtc accattgtgg taacgcattc aagcaacaca acctcattcg ggttaatgtc
     4141 tgcatgaatt aattcatcaa gatgttgcca gcgctccact gtgcgccagt gctccgggcg
     4201 gccttgccga tgatgttcta tccgtgcagc catctcatca tcaaggattt gcgaggtagc
     4261 gatatacaga acctgtgaag agtccccaat aagcgcctct gcgtggcgac tcttcccgct
     4321 ccgtgcgccc cccgtcacca aaatcatcat accgactcct gattcaaatg tatgatggta
     4381 ttcattgaac agaacaatgg atcccccacc gttgctgacc gcaaaacatt tacgcgatta
     4441 atactgcgcg taatataatt aaaaatccag cattctcaat ccattacgaa ataagatggc
     4501 attacggaat agccagactt tctcgtgtta acgtccgaca aaatgcgtca ttgacagcgg
     4561 tttctgtgga ttgacacatt tcattcagat aatgaattaa tgctactgcc ggaacaacca
     4621 gcaaacgggc attgtgttct gaaatccagg ctattgattc aacgtcagcg acagatctgc
     4681 gctgtaaaag aacttgtaaa acaacgtaat agaattatcc ggaatggtgg cgactatgca
     4741 ctagaacacc acaatttcgc tctctcggta aggcactgtc acagcattca acagaatgtg
     4801 acttgccaga tttattagcg ccaccagatg tttaaccggg taaccacgac ccagtcgggg
     4861 atatattcca tcttctcgct gacatcatca agaataaaaa ggtttatcac actcagagca
     4921 acgttcttca gcaggcggga taattcttca acatttagaa gatgtgttat taaaggcata
     4981 caaactggac gtattgtatt ttcttttggt ggttgaccta aggtagcagt ttatcctgat
     5041 gcgctgagat ttctgtaata tctacgtcaa cattctcctc gaatagaaaa tgcagccccg
     5101 gcaagtgatt cacatttttt aacaaaacgt tgttagcgtg ccattttcag aagctctcca
     5161 agaaagcgaa ttcttttctc aaattctgca ttctcagaca cagctttcta ctccatggta
     5221 atggcccaaa tacgaagttg ctcaggatca ttagtggtgc tgagaagtta aataaccatg
     5281 cggagtacca ctcgtcatca cgtatttata tatcatgaag ctattgttgt gttttgtaca
     5341 tcagtagata tattctgata tactcctttt gctagacata acctttcacc tgcttgcaaa
     5401 gcttctgtgt tctgacattg ccaaattgtt gcaattctgt atccagcctt ctttcagtca
     5461 tagcttcggg ccgcgataag actcactgat ctgaccctga ttcctcttgc agactttata
     5521 gaccaattaa aatgcagttt ctgcaggtca acgtctgacc atcattgtca tcactctggc
     5581 cattagagta accttctgca ttcatccttt tgtaaaaagt ttatattagt atcagcaatt
     5641 aaccggacct gatactgata tgagtcttac cgcatatacg gtcaatttca gcaattaatt
     5701 acattatcca cgccaaagta tttgtcatca caatgatggt accttctttc agacaccatt
     5761 ttttcaactc c
//
LOCUS       DAOTRK010000213         5736 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.215, whole genome shotgun sequence.
ACCESSION   DAOTRK010000213 DAOTRK010000000
VERSION     DAOTRK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.215"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(582..2963)
                     /locus_tag="QIE41_004388"
     CDS             complement(582..2963)
                     /locus_tag="QIE41_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDS3533180.1"
                     /translation="MKTLKNWKLQNQSAHHIELLVDGQHSLCLYILEENMFRVLLKRK
                     GVLSLDRTWSIAPEKDVPWEGRHREDISGFSLPTWNMEQNDELLTITTSSLRVIIHKP
                     LWLEWHYKDNAGQWQELVNDRPTSAYLINAHGDGVAHYQSRRNDERFYGLGDKSGDLQ
                     RTGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITHRSDISFGLFYDNLSNSWLDLGNEI
                     DNYHTTYRRWQAEAGDIDYYLFTGRCVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMQFIRLCKEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPHPKMMSQ
                     EFHNAGIHLAANIKPCLLQDHPRYNEVAEQELFIRDSEYNVPERSSFWDDEGSHLDFT
                     NPQTVAWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGKEIAIKHIRPVMPL
                     LMCRASMEAQQKFAPNKRPYLISRSGCAGLQRYVQTWSGDNRTNWDTLRYNTRMGLGM
                     SLSGLYNIGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPEV
                     TPAIRSAIELRYRLMPYLYTLLWQAHADDEPILRPTFLDHEHDVQTFEECDDFMLGRD
                     ILVASVVEAGQRQRRVWLPDNKTGWYDFYNGEWFCGGQWITIDAPLEKLPLLVRAGAG
                     IPLSERITYVSEAEDNHRKLKLFPIKGTGKSTGLLFEDDGETWGYTEGNALWLEWELD
                     CTATTIELRINTHGDYRPAWETLKVIIPQGESRQLLINGIEAYEWNMNLSCND"
     gene            complement(2978..4279)
                     /locus_tag="QIE41_004389"
     CDS             complement(2978..4279)
                     /locus_tag="QIE41_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3533181.1"
                     /translation="MSQDINNNIAVSKHRRIIRQLRWWMLILFLMGVTVNYITRNSLG
                     ILAPELKDSLGITTEQYSWIVGGFQLAYTLFQPLCGWLIDVIGLKIGFMICASLWGIA
                     CLLHAGAGSWIQLALLRFFMGGAEASATPANAKIIGEWFPKSERPVAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGALAILWVFLWWACYNTPEEHPNLSKNELNFIRQ
                     DNEAPPVKMPFLTALKTVSKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLAKAYGMDLK
                     QIALFAWLPFLCADLGSIASGYLTKLYTRLFGLSRVNSVIASSVSGAFLMVSLAVIAF
                     IDNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRKKRT"
     gene            4541..5611
                     /locus_tag="QIE41_004390"
     CDS             4541..5611
                     /locus_tag="QIE41_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDS3533182.1"
                     /translation="MEKKLKIADIAMRTGLSSSTVSRVLAGKANTSYRAREKVLACAR
                     ELGVMDGMASGRMLLNNLVIFAPQRAFDERTDIFYFRVIQSISKALSHYEVRLRYCAL
                     DEFDSTPSKFLARMNEAETQAAILLGIDDPHIHDLAADFSKPCVMINCHDRRMRLPTV
                     APDHKNIGAFASHFLFEMGHRRVMNIMCLRRYTMELRLAGIKEAWERQNQNFKDDRDL
                     LTINSFSAKEAEEKVGAWLDMTEKSMLPTAFLASGDFIAAGIINALKKRNIRVPQDVS
                     VMSIDGFNLAAIEDVPLTAVHVPRDELGTEAVHMLQQRLVRPDATVGALLLYGKLVIR
                     ESVRRIRPGKEPTPIKGDGLYD"
BASE COUNT         1711 a         1225 c         1157 g         1643 t
ORIGIN      
        1 acggtaaata tccataagtg tgggcttgcg gaataaatag tttgattaaa gataaggtgg
       61 tttaatactg ctgagtattt tataggagat atgatgttgt tttataaaca aactaaaaaa
      121 catgaagttt aattgatagg gggcaatgaa atctcctttt taaactcaat tgctatgttg
      181 ggtttatggg gttgctataa acggttttga ataaacgctt tactattctc atgaaaacag
      241 tgttatgttg ttattttaac aaccccgcat tgttttttga tgggatgggt attaagactt
      301 tagagaaact ttataaataa atatatgtat tcaatagtgg cgaaaataat aacccatctt
      361 attattacat tgtggaagtg ctctttttta ttatcgctca tgttttacct ttctgttaaa
      421 acatattctt tcggatatgt aaaatggtaa gagatagtta atctacgtat gcatgggggc
      481 gtttcattgc atggcgttat aatctggctt gtatataaag gcactataag ttttactctt
      541 acagtgcctt tatttggcca tgatttaccc tctacaataa attaatcatt acaggatagg
      601 ttcatattcc attcataagc ctcaatgccg ttaatcagta gttgacgact ttctccctga
      661 ggaattatca ctttcaatgt ttcccatgca gggcgataat ctccatgagt gtttattctt
      721 aactcaattg tagtggctgt gcagtccagc tcccattcaa gccacagagc attaccttca
      781 gtatatcccc aagtttcgcc atcatcttca aacagaagtc cggtggattt acccgttcct
      841 ttaattggga ataatttcaa tttacgatga ttatcttccg cttcactcac gtatgttata
      901 cgttcactaa gaggaatacc tgcacctgca cgtactaata atggtagttt ttccagtggc
      961 gcgtcgattg ttatccattg accaccacag aaccattcgc cattgtaaaa gtcgtaccat
     1021 cctgttttgt tgtcgggtaa ccatactcgt cgttgtcgtt gccctgcttc cacgacactt
     1081 gcaaccagaa tatcgcgtcc cagcataaaa tcatcacatt cttcaaatgt ctgaacatcg
     1141 tgctcatgat caaggaaggt tggacgtaat attggctcat catcggcatg tgcctgccac
     1201 aataatgtat ataaataagg cataagacga taacgtaact caattgcact acgaatcgcg
     1261 ggtgttactt ccggatacat ccatggttca ttgacagtat gatcatcatt ccacgagtgg
     1321 atcgtaaatc gggggtgcat aacaccgttc tgaacccaac gaacaaataa ttctggatca
     1381 ggcttatcac cagaaaaacc accgacatca tgaccaatgt tatacaatcc tgagaggctc
     1441 attcccagcc ccatgcgggt gttgtatcga agggtatccc agttagttcg gttgtcccca
     1501 ctccatgtct gaacataacg ctgcaatccg gcgcatccag agcgggaaat caaatatggt
     1561 cgtttgttcg gtgcaaattt ttgctgtgct tccatagatg ctctgcacat taaaagaggc
     1621 attactggcc gaatgtgttt aatggcgatt tctttgccaa aaccatgaca gcgcgcttcc
     1681 ccatcccata cttcaaactc attgttatca ttccaggtgg aatcaattcc catttcaagt
     1741 agttgtgtgg ttacaccttc ctgccaccat gcgactgttt gtgggttcgt aaagtccaga
     1801 tgagaaccct catcatccca gaagctggag cgttccggga cgttgtattc agagtcacga
     1861 ataaagagct cttgttctgc tacttcgtta tagcgaggat gatcctgcaa taaacatggt
     1921 tttatattag cggccaggtg tatccctgca ttatgaaact cctgactcat cattttaggg
     1981 tgtggaactt tgtcgtaatt ccagttgaat acgtagcgtt taccattaat agaagtataa
     2041 ccagaagata gctggaaaga atcacatgga atagcatgtt ccttacaaag acgaataaac
     2101 tgcatcaatt gattctgagc atccggtgca tctgtatagt gcatcgtgga accgctgtac
     2161 ccaagactcc attttggacc gaagagtgtt ttccccgtca aacgaacaaa ggctttggtg
     2221 atatcaagta cacatctgcc agtgaaaaga taataatcaa tatctcccgc ctcagcctgc
     2281 caacggcggt aagttgtatg ataattatct atttcattac ctaaatccag ccaactattg
     2341 ctgaggttat cataaaataa tccaaagcta atatcgctac ggtgagtaat tgtaaatgga
     2401 atatgtttat aaagagggtc tgtacttact gcattatatc ccattgcgtc caggttccgc
     2461 atttcatagc gttttcctgt gcgttgcaga tccccggatt tatcaccaag tccataaaaa
     2521 cgctcgtcat tacgtcgact ttgatagtgt gcaacaccat caccatgagc gtttatcaga
     2581 taagcactgg tagggcgatc attaacaagt tcctgccact gaccagcatt atccttataa
     2641 tgccattcaa gccataaagg cttgtgaata attacgcgta atgaactggt cgtgattgtc
     2701 agtaattcat cattctgctc catgttccag gtcggcagtg aaaaaccgct aatatcttcg
     2761 cgatgacgcc cttcccacgg gacatccttt tccggagcaa tgctccaggt tcggtccagc
     2821 gacaaaaccc ccttccgttt taatagaacc cggaacatat tttcttcgag tatatacagg
     2881 caaagagaat gctgaccatc aactaatagc tctatatgat gggctgactg attttgtagt
     2941 ttccagtttt ttaatgtttt catctccaca tactccctca ggtgcgtttt ttgcgacgtt
     3001 ctgcaataaa agtaaccaga aaaaaagcac caataagatc aaaaaatccc atggcgataa
     3061 atagcgggtt aaagcctatt ttgtcagcag taacgccaat aatcagtgag aacaaaaagc
     3121 tggcaatcca tgcggctgaa ccacgcattc cattaacagt ggccatctgc cccttatcaa
     3181 aagattcaac gaccagggca cttaacatgc aggaaattat ctgatgacca aaaccaccaa
     3241 tggagataag tacaatagca atatatggat tgtcgataaa agcaatcaca gcaagcgaaa
     3301 ccattagaaa tgctccgctt acagaggacg caataacaga atttactctg cttaacccaa
     3361 acagacgggt atagagtttt gtcagatatc cgctggcaat acttcctaaa tcagcgcaaa
     3421 gaaatggtaa ccatgcaaac agagctattt gtttaaggtc catgccatat gcttttgcaa
     3481 gatataatgg cacccaaaag ctcattacag cccatgctgg ctctgccata aatgcaggga
     3541 tagcgattcc ataaaagcgt ttatttttac taactgtttt taacgcggtc agaaaaggca
     3601 tttttaccgg aggagcttcg ttatcctgtc ggataaagtt gagttcattt ttacttaagt
     3661 ttgggtgctc ttctggtgtg ttataacacg cccaccaaag gaaaacccaa agaattgcaa
     3721 gtgcacctgt aaacataaat gcaccctgcc aaccaaatga cgcatgtgca aagtaaataa
     3781 ttggtggtgc aagcattgca ccgatagaaa aaccgacacc cgcccagcca gcagcaacag
     3841 gacgttcaga ttttggaaac cactcaccta taatttttgc atttgcaggt gtcgcagaag
     3901 cctcagcacc tcccatgaaa aaacgaagca acgcaagctg tatccagctt cctgctcctg
     3961 catgcagaag acaagcaatt ccccataatg aagcacaaat cataaatcca atcttgagcc
     4021 caataacatc aattagccat ccacaaagtg gctgaaaaag ggtatatgca agctgaaatc
     4081 caccaacaat ccaggagtat tgttcagttg taattccaag gctgtctttt aactctggtg
     4141 ccaaaatacc taatgagttt ctggtaatat aattgacagt gacacccatt agaaaaagga
     4201 ttaacatcca ccaccgtagt tgtcttatta ttcgacggtg tttgctgaca gctatattat
     4261 tatttatatc ctgactcata atcaccccac cggatgtaat aaatgatcga gcgacaacgc
     4321 agctcggggt tgccacatcc cactttacct ttcggcttaa tacttaaacc ggaactactc
     4381 attcacaata aaagaggggg gcgatgaaaa aaagttggta ttgtgcacgt tttttcataa
     4441 gtagttcata taaattctaa aaatagtcag aagtccttgc agtcaggcta ttccatccca
     4501 tgagaatttt ttcattttga aattagagat agatcacaat atggaaaaaa aactaaagat
     4561 tgctgatatt gccatgcgaa caggcctttc atccagcacc gtgtcacggg ttttagcggg
     4621 taaagctaat accagttatc gtgcccgcga gaaagtattg gcgtgtgcca gagagcttgg
     4681 ggttatggat ggaatggcat ccggccgtat gttgttaaac aaccttgtca tttttgctcc
     4741 tcaacgggct tttgatgagc gaacagacat tttttatttc cgcgtaatcc aaagcatcag
     4801 caaggcatta tcgcattatg aggttcggtt gcgttactgt gcccttgatg agttcgatag
     4861 tacaccttca aaattcctgg cgcgcatgaa tgaggcagaa acacaggctg caatattgct
     4921 tggtattgat gatcctcata tacatgatct tgcagcagat ttttctaagc cgtgcgtaat
     4981 gattaactgc catgatcgac gtatgcgtct tcccacagtt gctccagatc acaaaaacat
     5041 tggtgctttt gcgtctcact ttctgtttga aatggggcat cgcagagtaa tgaacattat
     5101 gtgtttacgt cgatacacaa tggaactgcg acttgccgga attaaagaag catgggagag
     5161 acagaatcag aactttaaag atgacagaga tttactcact attaatagtt tcagcgccaa
     5221 agaagccgaa gaaaaagtcg gagcatggct ggatatgaca gagaaaagta tgctaccgac
     5281 tgcttttttg gcaagtgggg acttcattgc tgcaggtata attaacgctt taaaaaaacg
     5341 taatatacga gtgccacaag atgtgtccgt aatgagtatt gatgggttta atctggctgc
     5401 aatagaagat gtgccactta cggcagtaca tgtacctcgc gatgaactgg gtactgaagc
     5461 ggtacatatg ttacaacaaa gacttgtacg tcctgatgca actgttggtg cattactttt
     5521 gtatgggaaa ctggtaatac gagaatctgt tcgccgtata cggccaggga aagagccaac
     5581 tccgattaaa ggagatggac tgtatgactg aaaccacagc ctctgattct tccggtcaag
     5641 ggaaaataag cataatgttc atcaccagat tatgtacgat gagttttcca ctgtttatag
     5701 caattatctt gctaataacc agacaggagt aaccag
//
LOCUS       DAOTRK010000214         5724 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.216, whole genome shotgun sequence.
ACCESSION   DAOTRK010000214 DAOTRK010000000
VERSION     DAOTRK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.216"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            398..787
                     /locus_tag="QIE41_004391"
     CDS             398..787
                     /locus_tag="QIE41_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533183.1"
                     /translation="MKARIKELEPYRALFIEKPVLAEHDDVIKQSEESETYSCLLRAP
                     AGLFLSFCLLWHYPIFPMLNTDTPEDILSLLGTLWPRGVTVRLPDMPAVQTTGLPGYL
                     FDRVRGQRCEIVRYVTSELFVGARLCK"
     gene            832..1002
                     /locus_tag="QIE41_004392"
                     /pseudo
     CDS             832..1002
                     /locus_tag="QIE41_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            1049..1183
                     /locus_tag="QIE41_004393"
     CDS             1049..1183
                     /locus_tag="QIE41_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDS3533184.1"
                     /translation="MSNISLYCLPYSGGSAAMYYKWRNVLSDNITLKPLEPVGKGNEQ
                     "
     gene            1173..2405
                     /locus_tag="QIE41_004394"
     CDS             1173..2405
                     /locus_tag="QIE41_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDS3533185.1"
                     /translation="MNSDFSAKKILTGEDVLCAAVHRIEWLFETFSSVCLSFSGGKDS
                     TVLLHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAG
                     NRCGVAILTGVRADESLNRFVGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKAR
                     DIWIYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            2390..3034
                     /locus_tag="QIE41_004395"
     CDS             2390..3034
                     /locus_tag="QIE41_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDS3533186.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVACINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPAWTVR"
     gene            complement(3405..5552)
                     /locus_tag="QIE41_004396"
     CDS             complement(3405..5552)
                     /locus_tag="QIE41_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS3533187.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEIVKGPYSVLYGSQAIGGIV
                     NFITKKGGNKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVNQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT         1471 a         1204 c         1407 g         1642 t
ORIGIN      
        1 aaatgggtct ttgataaaag tcaaaaattc tctcttttct tctgcgtcag ataacatcta
       61 agttatcaac tcttgtctgg cgtcggttac tttacggtta tgaattatct tatcctagcg
      121 atattgaatt gattcaaata acaggaaatg gtcgtagtac aaaggacaga aaatgatgtt
      181 gatagaagtg atggacagca cataaaatta tcgtagggtt ggcggcgatc gtccggcaaa
      241 tgctatcgac ggtattaaaa ccctgcacga aatcggcttc gataccctca aactgaatgg
      301 ttgtaaagaa ctggggctat tcgtgaagct tttggcaatc agattgagtc tggtcttgat
      361 ttccacgatt gcaccagcgt gctgatagtg gttgatagtg aaagcacgga ttaaagaact
      421 ggaaccgtat cgcgcgctgt ttattgaaaa gccggtgctg gcggagcatg atgatgtgat
      481 taagcagtct gaagagtcag aaacttacag ttgtctttta agagcacctg caggattgtt
      541 tctcagtttc tgcctgctct ggcattaccc tatttttccc atgttgaata cggatacgcc
      601 tgaggacatt ttgtctctgt tgggcactct ttggcctcgg ggagttactg ttcgcttacc
      661 ggatatgcct gccgtgcaga ccacaggctt acctggttat ttgtttgatc gcgtccgggg
      721 ccagcgatgc gaaatagtac gctatgttac cagtgagtta tttgtcggtg cgcgtctttg
      781 taaataaaga ctggcgttca ttgttatgca ttgatcatta tggcgaacaa tttacgttta
      841 cgcgtacgtg actatgtcat caccctggcg gtacccgtgt tcgtgaccgc catgctctgt
      901 atggcagcgg ttatactgta gcgttttatc ctgatatggg gtccatcgag tgtgttggct
      961 ataccgatag aaattatctt gttagctttc gttctgacat aacatcatca aattaaacga
     1021 attctattac acaacaagga gtgggaggat gagtaatatc agtttgtatt gtttgccata
     1081 ttcaggtggt tctgccgcta tgtactataa atggcgtaac gtgctgtcgg acaatattac
     1141 tttgaagcct ttagaacctg tggggaaggg gaatgaacag tgatttttcc gcgaaaaaaa
     1201 tcctgacggg tgaggatgtc ctgtgtgcgg ccgttcaccg tattgagtgg ttgtttgaga
     1261 cattttcttc tgtctgtttg tctttttccg gaggaaaaga ctctactgtg ctgctccatc
     1321 ttacggccga tgtggctcgc aggaagaaac gtcgtttctc tgtattattc attgactggg
     1381 aagctcagta ccagtgcaca attgcacaca ttctgaagat gagggagatg tatcgggatg
     1441 tgacggagac tttttactgg gtggcactcc ccctgactac ggtaaacggt gtctctcagt
     1501 ttcagccgga atggatatgc tgggaaccag gtgtggagtg ggttcgtcag ccaccagatg
     1561 acgctattac agatatgtcg tacttcccat tttatcggta tgccatgacg tttgaagaat
     1621 ttgttccggc attttcttcc tggtttgccg gtaaccggtg tggagtggca atactgactg
     1681 gtgttcgtgc tgatgaatcg ctcaatcgct ttgtgggact ggtgtctcag cgcaaactga
     1741 gatatgcaga tgataaaccc tggaccacag cgtcacctga agggttttat tacaccttgt
     1801 atccgttgta tgactggaaa gctcgtgata tatggatata taacgccaga acccgagcta
     1861 tctacaatcc cctgtatgac ctgatgtacc gtgccggcgt gccgttacgc aacatgcggg
     1921 tctgtgagcc ttttggcccg gaacagcgta agggactgtg gctttaccat gttctggagc
     1981 cggaaacctg ggccaggatg tgtgagcggg tgtcgggtgc tgccagcggg gcgctttatg
     2041 ccaatgagag cggtgcctat tttgccctgc gtaagcgtat cacgaagcca cctcatcata
     2101 cctggcgtag ctatgcgatg ttcctgctgg atgtgatgcc ggaaagaacg gcagaacatt
     2161 accgtaataa aattgctgtc tacctgcgct ggtatcagac gcggggcttc ccggatgaca
     2221 tcccggatga acaggagaat gacctgggga gccgggatat cccgtcctgg cgacgtatct
     2281 gtaagacact cataaagaat gatttctggt gtcggaccct ctccttcagt ccgaacaaac
     2341 cccggcacta tgaacgttat ctgcagcgta tgaaagaaag gaggaaggaa tgggggattc
     2401 tgtgacacct gaggtggaag tattgagcaa catgattcga cagtacttca gtcaggaacg
     2461 atctgaagaa gagaccatac gagcccttaa tcatctgcgc agggtgttgc atgaggtcag
     2521 cccttttgca caggagcctg tggactgtgt gttgtgggtg aaagcggatg aggtggtggc
     2581 caacgattac aatcccaatg tcatgtcttc cagtgaaaaa aaactgctta aacattcact
     2641 ggagcaggat ggattcacgc agcctgtggt ggtttcggaa gagaaggagc attatctggt
     2701 agtggatggt tttcatcgtc agttgctggg caggaaagcg gatacgagaa aacgtctgaa
     2761 gggctggctg ccagtggcct gtattaatcc agagagaaag ggacaggcat cacgtattgc
     2821 cgcgaccata cggcataacc gtgccagggg aaaacaccag ataacgtcaa tgtctgacat
     2881 tgtcagggac ctttcgcgtc tgggctggac agatgagcgt attggcacag agttgggcat
     2941 ggatcaggat gaagtgctga ggctcaaaca gattagcggg ctgacagagc tgtttcagga
     3001 agagaatttc agcccggcat ggacggttcg ttgatattgt aaaatatgat agttggagta
     3061 gggggcaata gaaggagaat atttattcca ccttcttccg cgtcgggata aatcaaaagg
     3121 tattatccat tgattcagta tctgggggag agtaaataat ctaaaaatag tattgcgttg
     3181 tgaactgata tcacttttga attacaaagg tataggctca gtagccagct ttataagtca
     3241 ttgtcgtagc aatgagtata gaatcatgct ggcgggaaga ataaatattc atctacagat
     3301 ggaaaacaac gacgaaaaac cttcaacaaa cacaatacgt tcgttagcct gcccgcaaag
     3361 cgggcagatc aggacaaaga attccataac gattgttttt gtcatcagaa attccataca
     3421 aaacctatag ctgcattaaa acctgctgca ggaatagttt catgtgctgt acggtagcgt
     3481 ttgtctgtca ggttattgag tgccaggtta atccggtact gatcctcgtt accgaattct
     3541 gtatttactg caaagttgag agtggcccag cccggaacat ttgtttcggt accgttactg
     3601 tcatcttttg cactagaggc agcacgaata aaaacatcag agattatgtt ggcctgaccc
     3661 atcacaagag tatgtttcag ccctatacgt ccgtttatcg ctggttcacc tgtattagtt
     3721 gtttttaatg ttgaagtttc atattgccga cgaattaaat tgccactgat atatggcgag
     3781 aaaacccagc cattatattc tgcacttatt tccagtcccc atgtttttgc ccgatcaata
     3841 ttgtcataat aatagtagct actacgggag gagttggtgt taccattgca aactatactg
     3901 ccatcacaga tcagacttgc aatataatct ttagcttctg agtagtaaac tgcgctgtca
     3961 atcagccact gattaccatt atatcgtgca cctaattcaa agttattgga gtgttcagcc
     4021 ttaagatcag gatttccgta tgtgacactg ccgcccgcag atgtctgcat aaaaagctgg
     4081 gagagtgtgg gaaatacgta gccttgcgcg aacgcagcgc gtaactccag gttatcgaaa
     4141 cctgaatagc gcaggcttgt agatgttacc atttcgtgat cactcgcaga ctctctggca
     4201 agagaggtat cgcttataat gcctgcggta tatgatactc cgtcaccacg cgtcaacttt
     4261 gaagaaagcc agtattggcg aactcccatt gtccatgtcc agtgatcggc gaattgccag
     4321 tcattttgtg cgaatagtga gactgtactt tgctctgact cataatagga gcgagttcgt
     4381 gtttccttat taatgaagcc ggtcagagat ttgcttgagg tcatgccacc ggacctttgg
     4441 ttgaccctgt cttgtttgta ctgtgcaccg gtaacaagtt cattattagc aggcagcgaa
     4501 aagtgactct gcaatgtgac cgcctgagtg tattgcttat catgggtgtc agtcttgtta
     4561 tgaacggtca gagcctgaat catcggactg ggaacaggct gtgtcgtttt tacttcgttg
     4621 gcaaattggc gctggatggt ctgctcatac gcgtcgaaat gaattttttt tagatagtca
     4681 ccgtccacgt ctgtgtcata gaatacccca actttctctc gttcaagttt aggtattttg
     4741 acactaaatg cctcataact tccgtctgga tcctcatagt aagtttgtgt cgcgagtctg
     4801 tagcgatcaa gcgagaggcc aaaacgatgg tttccggagt tataacccaa ccatacaccc
     4861 tgactattgt tacgatagtt ggtattcggc agacgtccat ccggcgtatc acgattgccc
     4921 tgatcagaat aactaccgtt gatgcgataa tcaaatccac cgatgctccc ctggaccgcg
     4981 attgattctt cccagcctgc tgttgcggaa ttataaacag ctttcacaac tccagatgca
     5041 agtttgttac ctcccttttt ggtgatgaag ttaacaatac cgccaattgc ctgtgaaccg
     5101 tacagtacgg aatatggacc tttcactatc tcaacacgct ccagcgcaga ctcatctatc
     5161 aacagtccca caccataatt atctccggcg cgctgataag ttacctcctg accatcaatg
     5221 agaattaaaa cacgggagga tgcttcgcca cgaatgcgga tttgtttacg gcctgccaag
     5281 gagttgtctg ttatctctac tccggggatg tcctgcaaat tatcggctat ggaggcattg
     5341 gtggagttct gcaatgtttg ttggtcaata acctgtatag tcgccgggct ttcccaaagg
     5401 ttgcttgccg aacgattcgt ggtcacgacg agcgtctctt cgttgctgat ttttgtttct
     5461 gaagaagttg cggttgcagt agaaactatc ccagaaatgc ataatgacag agtggaaagg
     5521 atataaattc gacaaggtat attatataac atttcatccc tcattaatac aaagtacaaa
     5581 tgattgctgt aatgataatt attttcattt atgttctcat atatctataa ttattgtcat
     5641 cttcataaat tgtaaatatc aaacattttt gaccttaatc atgttttttg atagatattt
     5701 tacgactgaa catatccgtc gttt
//
LOCUS       DAOTRK010000215         5699 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.217, whole genome shotgun sequence.
ACCESSION   DAOTRK010000215 DAOTRK010000000
VERSION     DAOTRK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.217"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(18..917)
                     /locus_tag="QIE41_004397"
     CDS             complement(18..917)
                     /locus_tag="QIE41_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533188.1"
                     /translation="MSQELSLTFTENIYYSTKEPVSIKDVITSLQGWEAIAKQSEGVL
                     QELTGANILDISVHVARLEAGSLYEDIVIKLLFGSQEEMDKFLAGAHAKIGNGKMRNA
                     LVGAVVIGLVGYGLVLATKAMAPSNTSHFEANNNTIINIGAGEANISPDRLQAIIEST
                     VTNKKTLAKSSIKTLAPARADEGSTMVIGTGGGTVTIPAETIKKAPTEVVFTPESYTQ
                     DHFDVDVEIRALDLDNPEKGWAAVIPGLIDRRVNMVLGPNVNPSDFAGKFAVRADITI
                     TYQLKSSDKKYQPKEVFIKEIIK"
     gene            complement(1047..1754)
                     /locus_tag="QIE41_004398"
     CDS             complement(1047..1754)
                     /locus_tag="QIE41_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3533189.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTFSIVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTIEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            1833..2060
                     /locus_tag="QIE41_004399"
     CDS             1833..2060
                     /locus_tag="QIE41_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3533190.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            2167..2466
                     /locus_tag="QIE41_004400"
     CDS             2167..2466
                     /locus_tag="QIE41_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533191.1"
                     /translation="MEQTNYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITNKKRPVCKTERSEQIQMEF"
     gene            2499..3398
                     /locus_tag="QIE41_004401"
     CDS             2499..3398
                     /locus_tag="QIE41_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDS3533192.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AGVTASKPKLDLTNTDWIYGVDL"
     gene            3395..4096
                     /locus_tag="QIE41_004402"
     CDS             3395..4096
                     /locus_tag="QIE41_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HDS3533193.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGSMK"
     gene            4336..>5699
                     /locus_tag="QIE41_004403"
     CDS             4336..>5699
                     /locus_tag="QIE41_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HDS3533194.1"
                     /translation="MTTVNKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSDN
                     KDVTQIYASDERANKVASFAVSAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNA
                     RIKLNVDNSFSLKDSSFDFLYPWLDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPD
                     NAMLGANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKDSRDIDDYLERP
                     ANGWDFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPL
                     IKFSAQHKQGQSNVHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNN
                     NIVLEYKKKHALKISLPESVQGDGESIIPVTLTINNASGGIKSVQWNDSVFTAAGGKI
                     SGNGTSWQVTLPAYKSEGVNSWNVGATVQDNRGNVSNYAVMNISVINSGVSTADSSFM
                     LDGDS"
BASE COUNT         1622 a         1222 c         1293 g         1562 t
ORIGIN      
        1 gctgaggccg ggtataatta tttgattatt tcttttataa agacttcttt cggttggtat
       61 tttttatctg atgatttgag ctggtacgtt attgttatat cagcacgtac agcaaattta
      121 cctgcaaaat ctgatgggtt tacgttgggg ccaagaacca tatttactct tcgatctatt
      181 aatccaggga taacagcagc ccaacctttc tctggattgt cgaggtctaa tgcacggatt
      241 tcaacatcaa catcaaaatg atcctgagta tatgactctg gtgtgaagac aacttcagtt
      301 ggggcttttt taatggtttc agcgggaatg gtcactgtac caccccctgt acctatgacc
      361 atcgtagatc cttcgtctgc cctagctggg gcaagcgtct taatggaact tttggcaaga
      421 gtcttcttat tggttaccgt gctttctatg attgcttgaa gacggtcagg tgaaatatta
      481 gcctcaccgg caccaatatt gatgattgta ttgttatttg cttcaaagtg ggaggtgttg
      541 cttggagcca tagccttagt tgctagaact aagccatatc caaccagacc aataacaaca
      601 gcaccaacga gagcgtttct cattttccca tttccgatct ttgcatgagc gccagcaaga
      661 aacttgtcca tttcttcttg gctaccaaag aggagtttaa taacaatatc ttcataaagg
      721 cttcctgctt ctaatcgcgc aacatgcacc gatatatcaa ggatattagc ccctgtcagt
      781 tcctgcaaaa caccttctga ttgttttgcg atggcctccc acccttgaag ggaggtaatc
      841 acatctttga tgcttacagg ttctttagtt gaataatata tgttttcggt aaaggttaaa
      901 gataattctt gggacatccg agccctcgct gtttctttcg atttgctgtg ttaagctaat
      961 attttaatat ccatgtttct gtttgcttgc agtgtgtttc tcaccaccat ccaaacgtac
     1021 tggttactag ctatgtgatg atgaagtcat gaacttttca gccattccct ggcctcgatg
     1081 tcatctaggt ggcgagattg cttcagaata ccagctacat actccacctt tgctacttga
     1141 tgataaggca acgttattgg cctgtggtct tggttgatgc ttgtaaattg gtattctccg
     1201 tctctgtcat agccaagaac cttgatcatg ttgtgccctt caatggttct gacaaacacc
     1261 tcatcacctg ggaatacttt ggtgttaggc tcaatgagta catattctcc tgattttatt
     1321 ctgggccaca tgctgtctcc ttttacacga agaccaaagg catctggatc atcgctataa
     1381 atcttgagcc acccatcgcg ctcttcggtc atctcgatgg caccatcaac accaagaatt
     1441 gcctcaccaa ccacgcgcac taaccctttt tttaatttgc caacaattga aaaagtatct
     1501 tcatcattcg ctccatttaa cgaagtgccg tgctgaagcc aaacaacatc aacgtttaga
     1561 aatttcgcaa gcgcattcat tttttcctgg cgtggtaaag actcagcatt aaaccatttg
     1621 ctaacgcctt tggacgaaag agaaagggca cgggctatgg ccattcccct accatgttca
     1681 tcaagaccag cttctttaca ggcttgcgct agccgctggg cgaattcttt gcgcactttt
     1741 tcattctgaa ccatgagtac gatactaaag cacttgcaaa aactttcagt tcaaccataa
     1801 tgcgtactga aagtacgaaa aaggacattc ctatgcaaaa tcttgatgag ccgattaaag
     1861 gtgtcggcat ccctgaagtt gcgaaggctt gtggagttag cgaaagggct gtctataagt
     1921 ggctcaaaaa cggcttcctc cctaagactg agttttttgg gaaaacgaaa tacgcatcaa
     1981 aaatcgaaga gatttctggt ggcaaatatc aagcaagcga aatgcttgaa ataagcaaaa
     2041 agaaccttct ggcagcataa gtaacaccgc tctttatcaa tctgcaccgc cgacaacgcg
     2101 gtaactaatt aagaactcat cgaaagatgc gtattagtga ttatttacct atggaaatag
     2161 taagaaatgg aacaaacaaa ttacagcaaa ctatcacagc gcgacgttga tcgcgcagaa
     2221 acagatttac ttatcaacct gtcaacgctt actcagcgcg gtctggcaaa gatgattggc
     2281 tgtcatgaat cgaagataag cagaacagac tggaggttta tagcttcggt cttgtgtgct
     2341 tttggcatgg catcagacat cagtccgatt agcagagctt ttaagtatgc gcttgatgaa
     2401 atcaccaata aaaaacgccc ggtgtgcaag accgagcgtt ctgaacaaat ccagatggag
     2461 ttctgaggtc attactggat ctatcaacag gagtcattat gacaaataca gcaaaaatac
     2521 tcaacttcgg cagaggtaac tttgccggac aggagcgtaa tgtggcagat ctcgatgatg
     2581 gttacgccag actatcaaat atgctgcttg aggcttattc gggcgcagat ctgaccaagc
     2641 gacagtttaa agtgctgctt gccattctgc gtaaaaccta tgggtggaat aaaccaatgg
     2701 acagaatcac cgattctcaa cttagcgaga ttacaaagtt acctgtcaaa cggtgcaatg
     2761 aagccaagtt agaactcgtc agaatgaata ttatcaagca gcaaggcggc atgtttggac
     2821 caaacaaaaa catctcagaa tggtgcatcc ctcaaaacga gggaaaatcc cctaaaacga
     2881 gggataaaac atccctcaaa ttgggggatt gctatccctc aaaacagggg gacacaaaag
     2941 acactattac aaaagaaaaa agaaaagatt attcgtcaga gaattctggc gaatcctctg
     3001 accagccaga aaacgacctt tctgtggtga aaccggatgc tgcaattcag agcggcagca
     3061 agtgggggac agcagaagac ctgaccgccg cagagtggat gtttgacatg gtgaagacca
     3121 tcgcgccatc agccagaaaa ccgaattttg cagggtgggc taacgatatc cgcctgatgc
     3181 gtgaacgtga cggacgtaac caccgcgaca tgtgcgtgct gttccgctgg gcatgccagg
     3241 acaacttctg gtccggtaac gtgctgagcc cggccaaact ccgcgataag tggacccaac
     3301 tcgaaatcaa ccgtaacaag caacaggcag gcgtgacagc cagcaaacca aaactcgacc
     3361 tgacaaacac agactggatt tacggggtgg atctatgaaa aacatcgccg cacagatggt
     3421 taactttgac cgtgagcaga tgcgtcggat cgccaacaac atgccggaac agtacgacga
     3481 aaagccgcag gtacagcagg tagcgcagat catcaacggt gtgttcagcc agttactggc
     3541 aactttcccg gcgagcctgg ctaaccgtga ccagaacgaa gtgaacgaaa tccgtcgcca
     3601 gtgggttctg gcttttcggg aaaacgggat caccacgatg gaacaggtta acgcaggaat
     3661 gcgcgtagcc cgtcggcaga atcgaccatt tctgccatca cccgggcagt ttgttgcatg
     3721 gtgccgggaa gaagcatccg ttaccgccgg actgccaaac gtcagcgagc tggttgatat
     3781 ggtttacgag tattgccgga agcgaggcct gtatccggat gcggagtctt atccgtggaa
     3841 atcaaacgcg cattactggt tggttaccaa cttgtaccag aacatgcggg ccaatgcgct
     3901 gactgacgcg gaattacggc gcaaggctgc cgatgaactg acctgtatga cagcgcgaat
     3961 taaccgtggt gagacgatac ctgaaccagt aaaacaactt cctgttatgg gcggtagacc
     4021 tctaaatcgt gcacaggctc tggcgaagat cgcagaaatt aaagctaagt ttggactgaa
     4081 aggatcgatg aaatagagct aaaatattag ctttaaaata aagtatatct agcgaatata
     4141 atccaggact ggttgtaatg cccgcaataa ttgattgcgt gactaataaa atttttcttc
     4201 cattgtaatc atttaaccat aaagtttatg tggatatttt atttgtgtgt tgattttttt
     4261 gtaacgttct gtatatttcg cacctcttgt cataccagtt atttttttca ttatattcat
     4321 aacgttgatt gaactatgac cactgtgaat aaaaagttaa aaaaaactgc atctggcgcg
     4381 attacatggt cagtaattgt tacccagata ttatctccag tttctctttc tttgattccg
     4441 gcaaacagtt ttgcatcgtc tgataataaa gatgttacgc aaatttatgc tagcgatgag
     4501 cgtgcaaata aagtggcctc atttgcagta agtgcaggtc agagtctggc gaataataat
     4561 gcaagtagtt ttgctgtaaa tactttatca actcaggcga caaaagaggt cgtcgattgg
     4621 ttgcaacaat atggcaatgc gcgcatcaag cttaatgtcg ataattcttt ttccttgaag
     4681 gattcatcat tcgacttttt atatccatgg ctggatactc aggattatgt gctattcagt
     4741 caaacatcac tacatcgtac tgatgaccgt aatcagacca atattggttt ggggattcgt
     4801 catttcactc ctgataatgc aatgttgggt gcgaatgttt tctacgatta tgatttaagt
     4861 cgcagccact ctcgcgcagg ttttggggtt gagtactgga gagattattt caggcttggt
     4921 gtaaatacct attttggttt atctgactgg aaggacagtc gggatattga tgattatctg
     4981 gaaagacccg caaatggctg ggatttttct gctgaaggat ggctacctgc ttatccgcaa
     5041 ttaggggcat ctattcagtt tgaaaaatat tatggtaaaa atgtcggatt atttggaagc
     5101 gataatctgc aggaaaaccc ttacgcagtt actgggggaa tttcttatac accagttcct
     5161 ctgattaagt tttctgcaca gcataagcaa ggacagagca acgttcacga tacaaccttt
     5221 ggtgttgagt ttaattaccg cccaggcgtt tcccttgctg aacagcttag cagtgacaat
     5281 gttgcagtta tgcgagaagt ccagaaccgg cgttatgatt ttgttgaacg aaataacaac
     5341 attgttctgg agtacaagaa gaagcatgca ctgaaaatca gcttaccaga gtctgttcag
     5401 ggggatggcg aatcaatcat ccctgtaaca ctgacaatca acaatgccag tggtggtatc
     5461 aagtctgtac agtggaatga tagtgtattc actgcggctg gcggtaagat cagtggaaat
     5521 ggcacatcat ggcaggtcac tttaccggct tataaaagcg aaggtgtaaa ttcgtggaat
     5581 gttggagcca cggtccagga taatagaggc aacgtttcca actatgcggt gatgaatatt
     5641 agcgttatta atagtggtgt ctcgacagcg gattcttctt ttatgttgga tggagattc
//
LOCUS       DAOTRK010000216         5695 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.218, whole genome shotgun sequence.
ACCESSION   DAOTRK010000216 DAOTRK010000000
VERSION     DAOTRK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.218"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            91..633
                     /gene="fimA"
                     /locus_tag="QIE41_004404"
     CDS             91..633
                     /gene="fimA"
                     /locus_tag="QIE41_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDS3533195.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            853..1545
                     /gene="fimC"
                     /locus_tag="QIE41_004405"
     CDS             853..1545
                     /gene="fimC"
                     /locus_tag="QIE41_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDS3533196.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1576..4185
                     /locus_tag="QIE41_004406"
     CDS             1576..4185
                     /locus_tag="QIE41_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDS3533197.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTVNTGDKSGGLMP
                     CFNQALLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNSSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4198..5205
                     /gene="fimH"
                     /locus_tag="QIE41_004407"
     CDS             4198..5205
                     /gene="fimH"
                     /locus_tag="QIE41_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDS3533198.1"
                     /translation="MKIICRLLLAMACLWLTNISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMKLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPNDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5216..>5695
                     /gene="sfmF"
                     /locus_tag="QIE41_004408"
     CDS             5216..>5695
                     /gene="sfmF"
                     /locus_tag="QIE41_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDS3533199.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAVDPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLSAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVA
                     "
BASE COUNT         1499 a         1225 c         1379 g         1592 t
ORIGIN      
        1 taaaaaggaa tttactacta tcagtgtctt aaataaagta atcggttata tacggatgtg
       61 gagtcgataa atgagattga aggaatatat atgaaattaa gatttatttc gtctgcgctg
      121 gccgccgcac tattcgccgc tacgggtagt tatgctgccg ttgtagatgg cggtacaatt
      181 cactttgaag gcgaactggt gaatgctgcc tgttcagtga atactgactc ggcagaccag
      241 gttgtcacac tcggtcaata tcgtaccgat attttcaatg ctgttggtaa tacctctgca
      301 ttaattccat tcactataca gttgaacgac tgcgatcctg tcgttgccgc taatgctgcc
      361 gttgcatttt ctggtcaggc ggatgcaatc aatgataatt tattggccat tgcatccagt
      421 actaatacaa caacagcaac gggtgtcggt attgaaatac ttgataatac atccgcaatt
      481 ctcaaacctg atgggaatag cttctcaacc aaccagaact tgatccccgg gaccaacgtt
      541 cttcattttt ctgcacgtta taaaggcacc ggtacaagtg catcagcagg gcaagcaaat
      601 gctgacgcga cttttattat gagatacgaa taatcaaaac cacgttgttt tgaattatat
      661 atcacgtctt ataacaaagt aatgtaccgg ttgtctgaag cggtatggtg gcaatgtaaa
      721 tcgaaatcat attcactttg tatcatgccg ctttattaaa tgaaaaggga atgatgtgtt
      781 gtaagaaacc aaagcaatca tttctttata ctccttattt ttgccgtcag gaatacacaa
      841 ggcgtattaa ctatgatgac taaaataaag ttattgatgc tcattatatt ttatttaatc
      901 atttcggcca gcgcccatgc tgccggaggg atcgcattag gtgccacgcg tattatttat
      961 cccgctgatg ctaaacagac tgcggtatgg attagaaata gccataccaa tgagcgcttt
     1021 ctggtcaatt cgtggattga aaacagcagc ggtgtaaaag aaaagtcatt catcattaca
     1081 ccgccactgt ttgttagtga acccaaaagc gaaaatactt tgcgtattat ttacaccggt
     1141 ccaccgctgg cggcagatcg tgagtctctg ttctggatga atgttaagac gatcccttcg
     1201 gtagataaaa atgcattgaa cggcaggaat gttttgcaac tggcgatttt atcgcgcatg
     1261 aaattatttc tccgtccaat tcaattacaa gaattacccg cagaagcgcc ggacacactc
     1321 aagttttcgc gatccggtaa ctatatcaat gttcataatc catcaccttt ttatgtcacc
     1381 ctggttaact tgcaagtggg cagccaaaaa ctggggaatg ctatggctgc acccagagtt
     1441 aattcacaaa ttcccttacc ctcaggagtg cagggaaagc tgaaatttca gaccgttaat
     1501 gattatggtt cagtaacccc ggtcagagaa gtgaacttaa actaaccgaa tcatctgaca
     1561 atatcagagc taattatgaa aatacccact actacggata ttccgcagag gtatacctgg
     1621 tgtctggccg gaatttgtta ttcatctctt gccattttac cctccttttt aagctatgcg
     1681 gaaagttatt tcaacccggc atttttatta gagaatggca catccgttgc tgatttatcg
     1741 cgctttgaga gaggtaatca tcaacctgcg ggcgtgtatc gggtggatct ctggcgtaat
     1801 gatgagttca ttggttcaca ggatatcgta tttgaatcga cgacagtaaa tacaggtgat
     1861 aaatcaggtg ggttaatgcc ttgttttaat caggcactcc ttgaacgaat tggccttaat
     1921 agcagtgcat tccccgagtt agcccagcag caaaataata aatgcattaa tttactgaaa
     1981 gctgtacctg atgccacaat taactttgat tttgcagcga tgcgcctgaa catcactatt
     2041 cctcagatag cgttgttgag tagcgctcac ggttacattc cgcctgaaga gtgggatgaa
     2101 ggtattcctg ctttactcct gaattataat ttcaccggta acagaggtaa tggtaacgat
     2161 agctattttt ttagtgaact cagcgggatt aatattggcc cgtggcgttt acgcaacaat
     2221 agttcctgga attattttcg cggaaatgga tatcattcag aacagtggaa taatattggc
     2281 acctgggtac agcgcgccat tattccgctg aaaagtgaac tggtaatggg agacggtaat
     2341 acagggagtg atattttcga tggcgttgga tttcgtggtg tacggcttta ttcttccgat
     2401 aatatgtatc ctgatagcca gcaggggttt gctccaacgg tacgtgggat tgcccgtacg
     2461 gcggcccagc taacgattcg gcaaaatggt tttattatct atcaaagcta tgtttccccc
     2521 ggcgcttttg aaattacaga tttacacccg acatcttcaa atggcgatct ggacgtcacc
     2581 atcgacgagc gcgatggtaa tcagcagaat tacactattc cgtattcaac agtgccaatt
     2641 ttacaacgcg aagggcgttt caaatttgac ctgacggcgg gtgattttcg tagcggtaat
     2701 agtcagcaat cgtcgccttt cttttttcag ggcacggcac tcggcggttt accacaggaa
     2761 tttactgcct acggcgggac gcaattatct gcaaattaca ccgccttttt gttagggttg
     2821 gggcgcaacc tcgggaactg gggcgcagtg tcgctggatg taacgcatgc gcgcagtcag
     2881 ttagccgacg acagtcgtca tgagggggat tccattcgct tcctctatgc gaaatcaatg
     2941 aacactttcg gcaccaattt tcagttaatg ggttaccgct attcgacaca aggtttttat
     3001 acccttgatg atgttgcgta tcgtcgtatg gaggggtacg aatatgatta cgattatgac
     3061 ggtgagcatc gcgatgaacc gataatcgtg aattaccaca atttacgctt tagccgtaaa
     3121 gaccgtttgc agttaaatat ttcacaatca cttaatgact ttggctcgct ttatatttct
     3181 ggtacccatc aaaaatactg gaatacttcg gattcagata cgtggtatca ggtggggtat
     3241 accagcagct gggttggcat cagttactca ctctcatttt cgtggaatga atctgtaggg
     3301 atccccgata acgaacgtat tgtcggactt aatgtttcag tgcctttcaa tgtcctgacc
     3361 aaacgtcgct acacccggga aaatgcgctc gatcgcgctt atgcctcctt taacgccaac
     3421 cgtaacagca acggtcaaaa tagctggctg gcaggcgttg gtgggacctt actggaaggc
     3481 cacaacctga gttatcacgt aagccagggc gatacctcga ataatgggta cacgggcagc
     3541 gccacggcaa actggcaggc cgcttacggt acgctggggg tcgggtataa ctacgaccgc
     3601 gatcaacatg acgttaactg gcagctgtct ggcggtgtgg tcgggcatga aaatggtata
     3661 acgctgagcc agcctttagg ggataccaat gttttgatta aagcgcctgg cgcaggcggt
     3721 gtacgcattg aaaatcaaac tggaatttta accgactggc gcggctatgc ggtgatgccg
     3781 tatgccacgg tttatcggta taaccgtatc gcgcttgata ccaatacgat ggggaattcc
     3841 atcgatgttg aaaaaaatat tagcagcgtt gtgccgacgc aaggcgcgtt ggttcgtgcc
     3901 aattttgata cccgcatagg cgtgcgggcg ctcattaccg ttacccaggg cggaaaaccg
     3961 gtgccgtttg gatcactggt acgggaaaac agtaccggaa taaccagtat ggtgggtgat
     4021 gacgggcaag tttatttaag tggtgcgcca ttgtctggtg aattactggt tcagtgggga
     4081 gacggcgcga actcacgctg cattgcgcac tatgtattgc cgaagcaaag cttacagcaa
     4141 gccgtcactg ttatttcggc agtttgcaca catcctggct cataaaggaa attatcaatg
     4201 aagataatct gcagattatt attggcgatg gcatgtttgt ggttaacaaa catatcctgg
     4261 gctactgttt gtgcaaatag tactggcgta gcagaagatg aacactatga tctctcaaat
     4321 gtctttaata gcaccaataa ccagccaggc cagattgttg ttttaccgga aaaatccggc
     4381 tgggtaggtg tgtcagcaat ttgtccaccc ggcacgctgg tgaattatac ataccgtagt
     4441 tatgtcacca actttattgt tcaggaaact atcgataatt ataaatatat gaaattaaat
     4501 gattatctat taggtgcgat gagtctggtt gatagcgtga tggatattca gtttcccccg
     4561 caaaattata tccgtatggg aacagatcct aacgtttcgc aaaaccttcc gttcggggtg
     4621 atggattctc gtttgatatt tcgtttaaag gttattcgtc cctttattaa catggtggag
     4681 atccccagac aggtgatgtt taccgtgtat gtgacatcaa cgcctaacga tccgttggtt
     4741 acacctgttt ataccattag ttttggtggc cgggttgaag taccgcaaaa ctgcgaatta
     4801 aatgccgggc aaattgttga atttgatttt ggtgatatcg gcgcatcgtt atttagtgcg
     4861 gcagggccgg ggaatcgacc agctggtgtc atgccgcaaa ccaagagcat tgcgatcaaa
     4921 tgtacgaatg ttgctgcgca ggcttattta acaatgcgtc tggaagccag tgccgtttct
     4981 ggtcaggcga tggtgtcgga caatcaggat ttaggtttta ttgtggccga tcagaacgat
     5041 acgccgatca cgcctaacga tctcaatagc gttattcctt tccgtctgga tgcagctgcg
     5101 gcagccaatg tcacacttcg cgcctggcct atcagtatta ccggtcaaaa accgaccgaa
     5161 gggccgttta gcgcgctggg gtatttacgc gtcgattatc aatgaggtac ggagaatgag
     5221 aagagtactc tttagctgtt tctgcgggct actgtggagt tccagtggat gggcagttga
     5281 ccctttagga acgattaata tcaatttgca cggtaacgtt gttgatttct cctgtaccgt
     5341 aaacacagcg gataaggata agacggtaga tttaggcaga tggcctacga cacaattact
     5401 gagcgctggc gataccacgg cactcgtccc ttttagcctg cggctggagg gatgtcctcc
     5461 gggttcagtt gcgattttat ttacgggaac gccggcatct gatgccaacc tgctggctct
     5521 ggatgatccc gcaatggcac aaaccgtcgc catcgaatta cgtaatagcg atcgctcccg
     5581 gctcgcactg ggggaggcga gcccgactga ggaagtagat gcaaatggca atgtcacact
     5641 aaactttttt gccaattatc gagcgttagc cagcggtgtt cagccaggtg tggcg
//
LOCUS       DAOTRK010000217         5649 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.219, whole genome shotgun sequence.
ACCESSION   DAOTRK010000217 DAOTRK010000000
VERSION     DAOTRK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.219"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..234
                     /locus_tag="QIE41_004409"
     CDS             <1..234
                     /locus_tag="QIE41_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533200.1"
                     /translation="KIPLTAQLIAKYKMDDQLDQLLVTREIQDEIKPKIQDKIDELSK
                     NLFNTINQTIKDNFDDIFRQQSENMSNYYEFVD"
     gene            complement(411..926)
                     /gene="pgpA"
                     /locus_tag="QIE41_004410"
     CDS             complement(411..926)
                     /gene="pgpA"
                     /locus_tag="QIE41_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDS3533201.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(904..1881)
                     /gene="thiL"
                     /locus_tag="QIE41_004411"
     CDS             complement(904..1881)
                     /gene="thiL"
                     /locus_tag="QIE41_004411"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDS3533202.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRAGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADVKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGASIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(1959..2378)
                     /gene="nusB"
                     /locus_tag="QIE41_004412"
     CDS             complement(1959..2378)
                     /gene="nusB"
                     /locus_tag="QIE41_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDS3533203.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2398..2868)
                     /gene="ribE"
                     /locus_tag="QIE41_004413"
     CDS             complement(2398..2868)
                     /gene="ribE"
                     /locus_tag="QIE41_004413"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDS3533204.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2957..4060)
                     /gene="ribD"
                     /locus_tag="QIE41_004414"
     CDS             complement(2957..4060)
                     /gene="ribD"
                     /locus_tag="QIE41_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDS3533205.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMESGESQWITSPQARLDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQHNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     ILPGLEKLADALQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4064..4513)
                     /gene="nrdR"
                     /locus_tag="QIE41_004415"
     CDS             complement(4064..4513)
                     /gene="nrdR"
                     /locus_tag="QIE41_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDS3533206.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4664..4792
                     /locus_tag="QIE41_004416"
                     /pseudo
     CDS             4664..4792
                     /locus_tag="QIE41_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            complement(4837..>5649)
                     /locus_tag="QIE41_004417"
     CDS             complement(4837..>5649)
                     /locus_tag="QIE41_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS3533207.1"
                     /translation="GWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRK
                     IQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAK
                     SGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVSPAYTSQRCTCCGHTAKENRLSQSK
                     FRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQEPTEMIQATA"
BASE COUNT         1366 a         1568 c         1372 g         1343 t
ORIGIN      
        1 aaaatacctt taactgcaca attaattgca aaatacaaaa tggatgatca attggaccaa
       61 cttttggtta cccgtgaaat tcaagatgaa ataaaaccaa agattcaaga caaaatagat
      121 gaactttcta aaaatttatt caacactata aaccaaacca ttaaggataa ctttgatgat
      181 atttttcggc aacagtcaga gaatatgagc aattattatg agttcgtcga ctgacgatat
      241 ttgagtgtac gtgaattatg agtgacaaag atacacctta aaatatcgcc gtataatata
      301 ttcggtgatg tcgggtttta ctttaacaga gtgccccaaa attggggcac tgttcattta
      361 taaatcaaac cgggtggtct ggaatcgcct acacctcccg gtcaatggca ttaaagaata
      421 cccagcggcc agtgatgacc gataaagtac aggatgcctg cggaaattac cccggcgaca
      481 atatcgtcga tcatgatccc catgccgcca tgtacattgc ggtcaaacca gcggatcggc
      541 cacggtttcc acatatccag gatacggaaa atcacaaacc cggcggcgac ccactgccag
      601 tcattggtcg gcagcgccat gagcgtgatc cacataccaa taaattcgtc ccagacaatg
      661 ctgccgtgat cgtgcacgcc catgtctttc gccgtttgat gacaaagata gacgccgata
      721 cagatcccca gcatcaccac cagcgaatag agctgccagg gcaaaaaggt catcagatac
      781 cagaacggaa tcgccgccag cgatcccatc gtgccaggaa cgattgggct taatccactt
      841 ccgaatccga cagcaagcag atgccacgga ttactcatct tcaggcgact tttcgcgaca
      901 tctttatggc gtggcaaaat ggtcatatcc tttccagtcg aacgtgacag gttcgccgtc
      961 acgaataaaa caaagccctt cgatatcggc ggtcatttgc ccgatacagg taaacggtac
     1021 gcccagatgt ccgagagcca catccagcgc gccacggttc agttccggca ccgtgaaaca
     1081 caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc gcctgttccg gttcaacatg
     1141 gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca atacttgcgc cgcagtcgct
     1201 ggctttcacg atatgcccga gatcggaaat cagaccgtca gagagatcga tagctgaatt
     1261 tgccagatcg cgcagtgcct gtccctgtaa aatacgcgga gatggacgga gatgacgttt
     1321 gatcaagtag tccgcatctt taacatcggc aacctgcaaa cggttttgca aaatcgccag
     1381 cccggcggcg ctatcgcccg gtgtaccggt cacatagatc cagtcacccg gtttcgcccc
     1441 agcgcgcgtt aaggctcgtc ccatcggaac aaagccgtgg atacccagcg tcattgataa
     1501 tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg taataattga gaagatcaaa
     1561 caaactgtcg ctgaaggact caagccacgc ttcgtcgacg tccggtaagg ttaatgccag
     1621 cgtcagccag gccggatcgg cccccatcgc agccagatcg cttaggttca ccgccagtgc
     1681 tttataagcc agatcagcag gatcgatatc agggaggaag tggttgcccg ccaccagcgt
     1741 atcagtgctg atcgccaggg tctgtttctc ggggatattg agaagtgcgc aatcgtcgcc
     1801 aatgcccagt tcgacatcaa gacgagaact tcttacacgg tcaaaataac gggcaatcag
     1861 ggagaactcg ccacatgcca tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga
     1921 tcgcggaacg gtcttccgtg aatctaccgg cctggatatc actttttgtt agggcgaatc
     1981 acaggtgctg ctttatcgag tacgccgttg acgaacttat ggctgtcttc tgcgccgaac
     2041 gatttcgcca gttcgatcgc ttcgttaatg gccactttgt atggcacatc gctacgttta
     2101 gacagctcgt acagcgcaat gcgcagtact gctttttcta cctggcccag ttcttccagc
     2161 aggcgggaca ggtatggctt catcagtccg tcgaggtatg cggtgttagt cgccaccccg
     2221 gccagcagct cacggaagta caggacgtca acgtctttta catcctgttc agccaggaac
     2281 tggtattcaa catcagcgat gtcgttctgg gacaactgcc aggagtagag cgcctggacg
     2341 gcacactcac gagcgcggcg acgagcagca ggtttcacgg atttcccctt actaatttca
     2401 ggccttgatg gctttcaata cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc
     2461 tttgttgcca gctttggtgc cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag
     2521 aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc
     2581 accaccagcg acatattcaa agtgggcagt gccaccacga ataaccgtac ccagcgcaat
     2641 caccgcgtcg tatttaccgg ttttagccag tgcacccgcc gccagcggca gctcataggc
     2701 accaggcacc caaacaacgg taatgttttc atcttttacc tgaccgatac gtttcagtgc
     2761 atcaattgca ccttccagca ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat
     2821 ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata atgttcattt tcttccttcg
     2881 ggttcgagta tggccccgca ggggggcgga ttttagcata atatttcgtg cgctgcttcc
     2941 ctttcgagcc gggagatcat gcacccacta aatgcaggca aacatccggg cctacatgac
     3001 gtatctcttt gaatttaaat tggagggcgt cagctaattt ctcaagccct ggtagtatgc
     3061 ataatccacg ggcgtcgctg cctaatagtt taggtgcgat atagacaatc agctcatcga
     3121 ctaaacccgc ctgcagcaat gcgccagcga gcgttggccc cgcttccacc cagatgctgt
     3181 taatctgctg tttacccagt tgcatcatta gtacaaccag atccagatga cctttatgct
     3241 ctggaatcag caaggtacgc accgtttccg gccactcacg agaatcttcc tgcgtacgcg
     3301 cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc
     3361 tatcaatcac aatacgtacc ggctggcgga gattgtgttg cgggtagagc ccctgagttt
     3421 gttcatccag ttcagaccaa cgcaccgtta aggccggatc atccgccagc accgtggcac
     3481 tgctggttaa aatggcatga ctttgtgcgc gcagtcgttg tacatcgagt cgcgcctgag
     3541 gcgaagtgat ccactggctt tcgccactct ccatcgccgt gcgaccatca agcgatgcgc
     3601 caagttttaa ctgaatataa ggaaagccgg tgcgcatccg cttgagaaag cctttattca
     3661 attgctcggc ttcactcatc atcaggccgt ggctgacgtc aataccagcc tgttgcaggc
     3721 gatatagccc tcgcccagct acctgcgggt taggatcttg catcgaggca accacgcgcg
     3781 ccacgcccgc agcaattaat gcatcacaac acggtggcgt acgaccatga tggctacagg
     3841 gttcgagtgt gacatacgcc gttgcgcctt tggctttttc gccagccatg cgcaacgcgt
     3901 gtacctcggc atgcggttca cccgctcgtt ggtggtaacc ttcaccgaca atttcgccat
     3961 ctttgacaat gacgcacccg acattcgggt tgggatgcgt ggtaaaacgt cctcgttgcg
     4021 ccagctttag cgcccgcgcc atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg
     4081 cgatctcttc gccaaattct ttgatatctt cgaaactgcg gtagacagag gcaaaacgga
     4141 tataggcgac tttatcgagc tttttcaatt gctccatcac cagattgcca atcatcttgc
     4201 tcggcacttc gcgctcaccc gtggcgcgca gctgcgattt aatatgattg attgccattt
     4261 cgacatcatc ggaactcacc ggacgttttt ccagcgcccg cagcattccg ctacgcaatt
     4321 tctcttcatt aaacggttca cgcacgtcgt tgctttttac aacacgcggc ataaccagct
     4381 ccgccacttc aaaggtggtg aaacgttcat tacacaccag acactgccga cggcggcgta
     4441 cagatgagcc ctcgcccacg agacgagagt caattacctt agtgtccacg gcgaaacaga
     4501 atgggcaatg catacggcgt cctgaccagg tggttaacag aaatctattt taccctgaac
     4561 tgacgataca acaaaggcgc aacgcttttg agataaagga tcgatgcttt cactgctttt
     4621 accctctacc attagactaa ttgcgacgag aaagggacga cctatgaata ctaacgtttt
     4681 tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc tgtgtgcagc aaagcgaagt
     4741 gcgacagatg aaacacagcg tcagcacgct gaaccaggag atgacgcaac tcattgacat
     4801 cctccacgcc ctgaaggacg tggatccctg ctacgttcag gctgtcgcct gaatcatttc
     4861 ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc cctccacagg caagaacggc
     4921 gtgccccgcc gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca
     4981 tacctggcat ctgaatttac tttgtgacag gcgattttct ttcgctgtat gaccacagca
     5041 cgtgcaacgc tggcttgtgt acgctggcga aacagcaagc acctgaccgc cacgccagag
     5101 ctgcttgtac tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt
     5161 taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt
     5221 tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt tgctgacggt
     5281 cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacgggaat gcagtcgctg
     5341 tattttgcgt ttctgcttct gccagttgtt gctgaatttg accttgcggc ttaactgtcg
     5401 ctgaagtctc gccagcgtct tctggttttt ctgaaaactg tttacaggct caaagactgt
     5461 gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca ttgatgctga
     5521 agggtgaacc ggagttgata cttcactttc tgtctgaata ctgatgtacc acttaccgca
     5581 ggactggctg accgtgacat ttttcacaac acccgtgacc tgacggctat tccggtagcg
     5641 catccagcc
//
LOCUS       DAOTRK010000218         5649 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.220, whole genome shotgun sequence.
ACCESSION   DAOTRK010000218 DAOTRK010000000
VERSION     DAOTRK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.220"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            57..248
                     /gene="hemP"
                     /locus_tag="QIE41_004418"
     CDS             57..248
                     /gene="hemP"
                     /locus_tag="QIE41_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDS3533208.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(252..1688)
                     /locus_tag="QIE41_004419"
     CDS             complement(252..1688)
                     /locus_tag="QIE41_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDS3533209.1"
                     /translation="MTLSFITRWRDELPATYTTLSPTPLNNARLIWHNAELANTLGIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVATSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQYSAASPLRDEFIDRAAFDDW
                     FAHYRVRLQQDEVTDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1751..2464)
                     /gene="ydiV"
                     /locus_tag="QIE41_004420"
     CDS             complement(1751..2464)
                     /gene="ydiV"
                     /locus_tag="QIE41_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDS3533210.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLFSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(2711..3175)
                     /locus_tag="QIE41_004421"
     CDS             complement(2711..3175)
                     /locus_tag="QIE41_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDS3533211.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(3253..4002)
                     /gene="btuD"
                     /locus_tag="QIE41_004422"
     CDS             complement(3253..4002)
                     /gene="btuD"
                     /locus_tag="QIE41_004422"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDS3533212.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(4002..4553)
                     /gene="btuE"
                     /locus_tag="QIE41_004423"
     CDS             complement(4002..4553)
                     /gene="btuE"
                     /locus_tag="QIE41_004423"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDS3533213.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYLDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(4616..5596)
                     /gene="btuC"
                     /locus_tag="QIE41_004424"
     CDS             complement(4616..5596)
                     /gene="btuC"
                     /locus_tag="QIE41_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDS3533214.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWS
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
BASE COUNT         1484 a         1511 c         1312 g         1342 t
ORIGIN      
        1 ggccgccata atattgataa taagaatcat tgttatatca attattatta atttttatgc
       61 gttatacgga tagcagaaaa ctcacgcctg aaacggatgc caatcacaag accgcttccc
      121 cgcagcccat ccggcgaatt accagccaga cactgttagg tccggatggc aaactgatta
      181 tcgatcatga cgggcaagaa tatctgctcc gtaaaactca ggctggcaag ctgctgttga
      241 ccaagtagcc tttaactcga gcagctgact tccagccgtt taccccagtc aggtggacga
      301 ctgacgtagt catcatctct gtcgctgaaa ggatttcgca acgcctcatg caggcggtgc
      361 aattccgtca tatcaccctt ttctgccgct tcaatcgccc gttgcgccaa ccagttgcgc
      421 aacaccagag ctgggttaac gctttgcatc agctgctgac gctcactatc ggtaacctcg
      481 tcttgttgca aacgtacccg ataatgggca aaccagtcat caaatgccgc acgatcgata
      541 aactcatcac gtagcggtga cgccgcgctg tactgctcgg tcagactcag catgcggaat
      601 gtgcgggtat aatcgctgcg ctctcgcgcc atcagactga ataattcatt cagtagcgcg
      661 ttatcctctt tttgctccgt catgaagccc agtttctgcc gcatccgttg tccataatgc
      721 gtcaacaaaa cctgctgata gctatccagg gcctcattca gggcatcaac ggcgacaaat
      781 ggtgacaatg tctgcgccag acgctgtaaa ttccacaacg cgacggcagg ttgattatca
      841 aagctgtaac gcccttggtg atccgagtga ttacaaataa aaccgggttc gtaatcatca
      901 agaaaaccaa acggcccgta atcaagcgtc agccccaaca gcgacatgtt gtcggtattc
      961 atcaccccat gagcaaagcc gaccgtctgc cattgggcaa ttaacgatgc ggtacgtgcg
     1021 acaacatcgg taaaccagag acggtatttg tcctcatcat ctgcaagatg tgaccagtaa
     1081 tgacgaatgg caaagtcagc taactgacga accttttccg gctcgcggcg atagtaaaaa
     1141 tgttcgaaat gaccaaagcg cagatgactt gttgccacac gcatcagcat cgcgcctggc
     1201 tccaccgttt cccgatacac tggcgaatcg ctggtgacga tacttaaagc gcgggtcgtc
     1261 ggaatgccca gataatgcat cgcctcactg gcgagacttt ctcgtatcgt cgaacgtaaa
     1321 accgcccgtc catcacccat tcgcgaataa ggcgtcaggc cagcaccttt cagatgccag
     1381 tccattgtag tgccatcagc aagccgttgt tcgccgagta aaatgccgcg cccatcaccc
     1441 agttggcccg cccagacgcc gaactgatga ccactgtaaa cctgcgccag tggtgacata
     1501 ccaggcagta gggtttcgcc accccagaca cctgcgccat ttttaaacag tgacgatgga
     1561 atacccagcg tgttagccag ttcggcatta tgccaaatca gccgggcatt atttaaaggc
     1621 gtaggggaaa gtgttgtata ggttgctggc aattcatcgc gccaacgggt aataaaagac
     1681 agggtcatag atcctcctgt ttgatagtgt agacggttac tctcgtctta aacaccagca
     1741 aacaggaggg ttatcgctga accaacgtcg ttatctgatt tatgggaacg gcaggccaca
     1801 ggcatccctg gagagcatgg aaatcaaagg gggtaatctg tgccattatt tctggcgtat
     1861 caatgccccc cgcgatgatg caattgcaac atggggaaat ttgcgcctgg atcgcacgaa
     1921 taaacggttc gaaggagcga tgcgtgattt gttgctgaat aaaactttta tccagcatga
     1981 cgcgggtgaa aagaccatca aaaaccgctt tcattgtgct attgcctgcg cccaaattac
     2041 ctaacacaag gggatacatt tgcgataagg aaaaaagatc gcgattatct tttccttcat
     2101 cgagatgcgg gtaattctca ttaatcaaca gttcaataaa cggatatttt aatagttcgc
     2161 ctgcgtaatt atccctgtct aataataaag tagccacttg tggcgttaaa ttaagccaag
     2221 caaagagttt atgttgaata aaaaagtgtt ggcatgattt tagtaattcc aactgctctg
     2281 aaaataattg ccaatgttgc tcctcggtaa gttgcgctat aacccgactc gtcgggatac
     2341 gaaccgtacc atcttcactg gagaaatggg taattaactc aacgccgaca aggtcttgct
     2401 ggttatctct tatcggcagg aaatagcaat cagaatgata aagattctcc aaaaaaatct
     2461 tcatggtaac cgtccctctc gaaggatgtt tttcagtatc cggctaaaag ggatgaggcc
     2521 ataagacata acaattacag aaggagtaac tttcatttgt tccatgttaa ccactttttc
     2581 aggggtcctt ttattagatt atcctgatta taaacgaata atccatttac ggaatttttg
     2641 tctgtaaaat actactgtat tcaggggtaa atggacggag atgtatcatc gccgtccatt
     2701 tttcgacata ctagattcgt ctcgcctgcc agaaattttt ctgccaatag acattatcga
     2761 gtgaggaacg catcactccc ttgctggtag aggcgtggat aaattggttg ttggtatcgt
     2821 aaatacctac atgcaaacca ttttgtccgg aacccgtttt gaaaaagacc aggtcaccag
     2881 gcagcaactc gtctttatca atttgcgtgc cgatagaggc ttgttctttg gtttctcggg
     2941 gcagctgcaa atcgaaacga tcgcgcatcg tcacaaccac aaatcccgaa cagtccacac
     3001 cgcgccgcgt catgccacca taacgatacg gcgtgccatg ccagctttgt agctggtcgt
     3061 tcaaaccggc aataacggta atcgaatcag aaagtctggc atttggcggc ggtgctttat
     3121 ggtggctaca cccggccaga aacagtgctg tgatcaaaat aaggcagaaa cgcattccgt
     3181 acggttcctc tgttttttat tcttgcatta atttagcgtc gtaattaccc gattttcaag
     3241 atactaatga aatcagatgg tcgaaatcag cattctgtga ccttcgatat ccagacggcg
     3301 aaaattcatc ccataggcct gcgccagatt tggcggcgtg agcacctctt ccctgcgtcc
     3361 actggccagc atttttccac cttttagcaa ccatgcccga tgcgcatgac gcaatgtatg
     3421 gttgagatcg tgactgctca tcacaatcgc cagtccttgc tgacacagcg cgctcagaat
     3481 tttgtctaac gcactttgtt gcgcaacatc aagactgttc atcggctcat caagaagcag
     3541 caattgacct gcgggattgg cttgtggtgt gatttgcagc accaccgcag caagacgtac
     3601 gcgttgccat tcgccgccgg aaagttgatt ggtgctacgt ccgagtttgt catcaagagc
     3661 cagcgcccct gcgacatcat tcagtagttc ggtacgcgtt ttatcgtgct gatgcagtgt
     3721 caggtagtgc cagaccggca ttgcaaacgg tggcgtctgt tgttgtgaaa gataggcgcg
     3781 atgcagcgcg agttttgttg cggaccatgc ttccagtggt tgccccgcga actgaatgct
     3841 tcccttaccg ctggtcattc cggccattcg cgccagtaag gtactcttac ccgcgccgtt
     3901 cggccccacc agatgcagga tctccccagc ccgaacctcg ccagaaagcg gccccaggcg
     3961 ggtagattcc gcaacatcct gtaactgcat cacaatagac attatttcgc caacgccagt
     4021 ttaatgcttt ccatcacaat gggatcttcc ggcgtcatat ccggggaaaa acgctggatg
     4081 accaatccgt ccctgccaac taaaaatttc tcaaaattcc ataaaatatc atcaaggtac
     4141 agcggtgcac gacctttgct gaccatacgg gcatagaatc cgctctcttc cggcgcgact
     4201 gcggtcggcg ctgcggcaat caatttttga tacagcggat ggcgtccttc gccattaact
     4261 tcaatcttac tgaacatcgg gaacgtcacc ccccatgtag tggtgcagta agttttaatc
     4321 tcttcatcgc tgcccggttc ttgctccaga aactggttgc acgggaatcc cagcaccaca
     4381 aaaccacgat cggcccaggc tttctgaata ttctccaact gctcatattg cggcgttaag
     4441 ccacactttg aggcgacatt gacaatcaac agcacattac cggcgtactt ctccagcgtg
     4501 gtcacttcac cgtcgatatc tttcactacg gtcgtcagaa tggaatcttg catcgtttct
     4561 cctgggtgtg gtcagtaaaa atcttagctt ttaatcatag accgtctttt tgcggctaac
     4621 gtcctgcttt taacaataac cagataaaca ccggcgcacc caacgttgcg gtgaccacgc
     4681 caataggcag ctctgcggca gctaatgcca ggcgcgctac aatatcggcc agcagcaatg
     4741 cgctcgcccc tgccagcgcg cagccgggaa gtaatacgcg atgatcggtt aaaccacaca
     4801 gccggagaat atgtgggata accagaccaa taaagccgat agcacccgcc agcgccacac
     4861 tgacgccaac catccagccg gtcgctgcca ccagcacatt gcgccagaac cacaggggta
     4921 aacccagttg ccgcgccgag atctcgccaa gtgctaacat attcatcggc ctggactgac
     4981 aacagatcca caacaacacg gggatcaatg ccagcatcag ccagctttgc cgccagtcta
     5041 cgccgccaaa accgcccatc atccagtaca tcagctgacg taaatcaaca gaggtggaaa
     5101 agtagatagc ccacgtcatt agtgcgctac agataatccc taatgcaacg ccagccagca
     5161 ataaccgact ggtcgaaaga tgacgacggg cgaaacgtaa gagtattaaa gtgatgataa
     5221 gcgcgccagc aatcgcacac agccctagtg cccagtttgg gagttgccct tgcccaagca
     5281 acaccgcggc gataagccct acgcctgcgc cgttagagac gccaagtagt ccaggttctg
     5341 ccagaggatt ttcaaacaac gcctgcatta ctgcgccgga tatagccagc gccgcaccaa
     5401 ccagcaatac agccagcgta cgtggcaggc gaatttgcca gacgaacagt tcgccacgag
     5461 gagtagacca gtcaccgggc gaaatccatt gttcaccggc gcaaaggctt aagagaagcg
     5521 ccagcagcat caaaactgac aggcataata accagcgaat attttgtcgc tgttgttggc
     5581 gggcaagtgt cagcatggta tccgttctgc tgaagtgtca tggcgttgat tttacggtga
     5641 ctcttcgac
//
LOCUS       DAOTRK010000219         5632 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.221, whole genome shotgun sequence.
ACCESSION   DAOTRK010000219 DAOTRK010000000
VERSION     DAOTRK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.221"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..93
                     /locus_tag="QIE41_004425"
     CDS             <1..93
                     /locus_tag="QIE41_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDS3533215.1"
                     /translation="ECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            109..585
                     /locus_tag="QIE41_004426"
     CDS             109..585
                     /locus_tag="QIE41_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDS3533216.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALHHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            648..869
                     /locus_tag="QIE41_004427"
     CDS             648..869
                     /locus_tag="QIE41_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDS3533217.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTETYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            888..1532
                     /locus_tag="QIE41_004428"
     CDS             888..1532
                     /locus_tag="QIE41_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDS3533218.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGHWHWQPATPRKIR
                     EYAPQDRELHRIADTLHAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENKYDWLTSDTAVDEALLINEYTFTEADLRAG"
     gene            1582..1962
                     /locus_tag="QIE41_004429"
     CDS             1582..1962
                     /locus_tag="QIE41_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDS3533219.1"
                     /translation="MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLLQEGNRLHYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYLAVYPATETESNPPE"
     gene            2039..2416
                     /locus_tag="QIE41_004430"
     CDS             2039..2416
                     /locus_tag="QIE41_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDS3533220.1"
                     /translation="MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            2413..2901
                     /locus_tag="QIE41_004431"
     CDS             2413..2901
                     /locus_tag="QIE41_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533221.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            2918..3115
                     /locus_tag="QIE41_004432"
     CDS             2918..3115
                     /locus_tag="QIE41_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDS3533222.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLQQNR"
     gene            3200..4045
                     /locus_tag="QIE41_004433"
     CDS             3200..4045
                     /locus_tag="QIE41_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDS3533223.1"
                     /translation="MSDITISRPELVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTARDWAMKQDFRCGCRLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQAHDTWYRSLEYDNFPEISEANILSTFEQLHQNKDDVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            4114..4509
                     /locus_tag="QIE41_004434"
     CDS             4114..4509
                     /locus_tag="QIE41_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDS3533224.1"
                     /translation="MTTKDRIQSQLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKGQ
                     STQIPVSLGIKTPSRFKRMLIVGKSKINA"
     gene            4502..5435
                     /locus_tag="QIE41_004435"
                     /pseudo
     CDS             4502..5435
                     /locus_tag="QIE41_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
BASE COUNT         1510 a         1452 c         1362 g         1308 t
ORIGIN      
        1 gagtgttatg ccatgacggt ccattattac cggctgcggg attacgccct gcagcatccg
       61 gaatgcagcg ccattatgcg catcatcgac tgaatggaga gaagtacaat gcaacaactt
      121 tcctttctgc ccggagagat gacgcccggc gagcgcagcc ttattcaacg ggccctgaaa
      181 accctggacc gccatcttca tgaacccggc gtggccttca cctccacccg tgcggcacgg
      241 gaatggctga ttctgaacat ggcgggactg gaacgtgaag agttccgggt gctgtatctg
      301 aacaaccaga atcagctgat tgccggtgaa accctcttca ccggcaccat caaccgcacg
      361 gaagtccatc cccgggaagt gattaaacgc gccctgcacc acaatgccgc agccgtggtg
      421 ctggcacata atcacccgtc cggtgaagtc acacccagca aggcagaccg gcttatcacg
      481 gaacgtctgg tacaggcact gggcctggtg gatatccggg tgccggacca tctgatagtc
      541 ggtggcagcc aggttttctc ctttgccgaa catggtctgc tttaacccgt cacaaccaca
      601 tcacacctgt tttcactttt atcttctgtc ttcagaggta tcccattatg aaaatcatca
      661 cccgtggtga agccatgcgt attcaccaac agcatcctgc atcccgtctt tttccgttct
      721 gtaccggcaa ataccgctgg cacggcagca ctgaaacgta taccggtcgt gaagtgcagg
      781 atattcccgg tgtgctggcc gtatttgctg aacgccgtaa ggacagtttt ggcccgtatg
      841 tccggctgat gagcgtcact ctgaactgag tcagtaaggg cattctgatg agcagaatca
      901 tcaccaccac cgtgtataca ctgcatgagc tatcctccac cgcacaggaa aaagcccgcg
      961 actggtaccg tcagcatcat gctgacagca actggtatga aaacgtgtat gaggacttca
     1021 gggaggtctg tggcattttc ggaattgacc tccgccagcg cgttttccgg ctcagcaacg
     1081 gcaggtttat ggaagagccg tgcatctggt tcagcggatt ctgcagccag ggggacggag
     1141 cctgttttga agggcactgg cactggcagc cagcgacacc ccggaaaatc agggaatatg
     1201 cccctcagga ccgtgagctt catcgtatcg cggataccct gcatgcggtg cagaaacgta
     1261 acttctggca gcttcaggct gaaatcagtc accggggacg ttactgccat ccgtacagca
     1321 tggatatcac cgtcacccgg aacagtccga ctggtcaggc aatgacaacc gatgcggaag
     1381 ccgctgtcag cgaagccctg cgcgacctgg ccttctggct gtaccgccag ctggaaaaca
     1441 agtacgactg gctgacctca gacaccgctg tggatgaggc ccttctcatc aacgaatata
     1501 ccttcaccga agccgatctg cgcgccggct gacccgtcat cgttaaacta tcattcaccg
     1561 attttctgtg aggattttat catgtcggac acactccccg gaacaacgct tcccgacgac
     1621 aacaaagacc gcccctggtg ggggctgccc tgcaccgtga cgccctgttt cggcgcccgt
     1681 ctgttgcagg agggtaaccg gttgcattac cttgcagacc gcgccggtat caggggccgg
     1741 ttcagcaatg cggatgcata ccatctggac caggcttttc cactgctgat gaaacaactg
     1801 gaactcatgc tcaccagcgg tgaactgaat ccccgccatc agcataccgt cacgctgtat
     1861 gcgaaagggc tgacctgcga tgccgacacc ctcgggtcct gtggttacgt ttatctggct
     1921 gtttatcctg cgaccgaaac ggaaagtaac cctccggaat agtcttctgc tccggcctgg
     1981 tgctttctcc acgccacttt tccatttctt atatctgcat atcaggaaaa tcttcagtat
     2041 gaaaacatta cccgacacac acgtacgggc ggcatcgcgc tgcccgtctc ccgtcaccat
     2101 ctggcagaca ctgctcaccc gactgctgga ccagcactat ggcctcacgc tgaatgacac
     2161 accgttcgct gatgaacgtg tgattgagca gcatattgag gcagggattt cactgtgtga
     2221 cgcggtgaac tttctcgttg aaaaatacgc gctggtacgt accgaccagc cgggattcag
     2281 cgcaggagcc ccgtcgcagt taatcaacag cattgatatt ctccgggctc gcagggcgac
     2341 cggcctgatg acccggaaca attacagaat ggtaaataac attacccagg gcaagcatcc
     2401 ggaggcaaaa cgatgaaact ggccctgacg ctggaagctg acagcgttaa cgtacaggca
     2461 ctgaacatgg ggcgaattgt cgttgacgtc gatggtgttg agcttgccga actgattaac
     2521 gtggtctgcg ataatggcta ctcccttcgt gttgttgatg aatctgaccg gacctcaaca
     2581 gacagcatac ccccctctgc tgccctgagc ggtatacgct gtagtaccgc gcatatcacg
     2641 gaaacagaca acgcctggct gtactcgctg tcgcatcaga cgaatgacac cggcgaatca
     2701 gaatggattc attttacagg tagcggatat ctgttacgta ccgatgcgtg gtcatacccg
     2761 gttctgcggc ttaaacgcct ggggctgtca aaaacgttcc gccgtctggt tgtcacactt
     2821 acccgacgtt atggcgtcag tctcattcat ctggatgcca gcgctgaatg cctgccggat
     2881 ttacccactt tcgactggta acccggaaat tcacaacatg aaatcattaa ccacggaaac
     2941 agcgctggat attctgattg cgtggctgca ggacaatatt gactgcgaat cagggattat
     3001 cttcgacaac gatgaggata aaacggattc agcagcactg ctgccctgca ttgaacaggc
     3061 cagggaagat atccgtaccc tgcgccaact gcagcttctg caacagaaca ggtgagtctt
     3121 actcaccaga ctttattcca ctgacgccag cctgaatacg gctggcgttt tcgtttataa
     3181 accaaaagga aaaacgatga tgtcagatat cacaatctcc cgcccggaac tggtcaacgg
     3241 acatacggac gttatctgct caactagtat ccgccacatt ctggctgtac gaaagagtgc
     3301 gctgctgcaa atcgacacac ttatccggca actggctgaa atctcggtac tgacggaaag
     3361 cattggcggt aaaaccgccc gggactgggc catgaaacag gattttcgct gcggctgcag
     3421 gctcatggag aaaccggaaa ccgcaatgaa agccatcacc cgcaatctcg atcgcgaaat
     3481 ctggcgtgac ctgatgcaac gttccgggat gctgtcatta atggatgcgc aggcccatga
     3541 tacatggtac cgctcactgg agtacgataa ctttccggaa atcagcgagg cgaatatcct
     3601 gagtacgttt gaacaactgc accagaataa ggatgacgtg tttgagcgag gtgtgatcaa
     3661 cctcttcaga ggtctgagct ggaattacaa aaccaattgc ccctgtaaat ttggcaataa
     3721 aattatcgtc aacaatctgg tgagatggga ccgatgggga ttgcatctta tcaccgggca
     3781 acaggcagat cgactcgctg acctggaaag aatgctgcat ctgttcagcg gcaaaccgat
     3841 ccccgacagc cgggaaaata tcatcattcg gctggatgat cacatccgct ccgttcaggg
     3901 caaagcgtgc tatgaagatg agatgttcag catcagatac tttaagaagg gctccgcaca
     3961 catcacgttc aggaaaccag aactggttga caggctaaat gacataattg ccaggcacta
     4021 tccaaatgta ttagcagaca ggtaatgtgg ccttgccccg ctattaaaca tgtcatattt
     4081 ttgctatcat tgtgaaaatt tttcggagga aacatgacta caaaagaccg tattcagtcc
     4141 caactgaacc gttcaaaacg ttatgtgttc acccgggatg atttcaggga tatcgctggc
     4201 tatgatcagg ttggcagagc tcttcgtaca ctggtaaacg aaggtaagtt gatgaaagtg
     4261 ggctatggtg tttacaccaa agcccgtcgt aacatcatca ctgggaaaat catgcccgca
     4321 gcgcctgggg gctctgctgc agtaattaca gaggccctgg agcgcctgaa tatcagatac
     4381 cgtctggcag gagcaacgga atgctacaat aaaggtcagt ccacacaaat tcctgtatct
     4441 ctggggataa aaacaccttc tcgatttaaa agaatgttaa tagtagggaa aagtaaaatc
     4501 aatgcctgaa ttaacatcat tatttcctga cgttgccgat gcactggata ttgaatctgt
     4561 cgctatcgtc gaaaaagatt attttgtggt tgatctttta agattattaa aagaaataaa
     4621 accagaaaca catactctcg tgtttgcagg aggtaccgca ttatcaaaag caggtatctc
     4681 gctaaacaga atgtcagaag atattgatat aaagctcgtg ccaacagaga actttatgca
     4741 aaatggcaga gacaagagaa gaaaaattcg caaggaaatc gtgcaaataa tcacggatgt
     4801 tattcataat tctgacattt tcagcttgga taatgaaaac gcccgtataa cacgtgatga
     4861 gtatcgttat aacgaaatct cggtacgtta tccccagaca tttgcacaag tcccctgtct
     4921 tcgtccattt attaagctgg agttgatgga aagcactctg ctggaacatc cggaaagcag
     4981 agatatttat tcccttgtca cggagttaac gggtaaaggt acacctgtaa cagcattccc
     5041 ctgtgccacc attttatcca ctcaggctga aaagctcatc tccatgatgc gaagaacagc
     5101 agcacatctt cgtaatcccg aacaacagga tgatgagttt ctggtacgtc acatttacga
     5161 caactactgc attgtcaggg aaaaaggtgt gaatgttcct gtattgaaaa attttgttca
     5221 gatatgtatt cagctatggt aaccagtacc cacaattttg tagctcacca gttgatgaac
     5281 ttaagaaagg gctggatgca ctccgggatt atcctgtaca caaattgcgc tttcaacgat
     5341 ttgttaaacc gatggtattt ggtaatacac agattaactg ggaagaagcc tattccagtt
     5401 tcagacaaac agcgctaagt gttctgacca gctaacaaac gcgttaagta cgattccaca
     5461 attcagcata tcgaaccaga ccaaatgatt acagcaaagc acaaaaaaga gggattatag
     5521 cgtcaaaaga attcgaattg cacatggggg cctcattggt ggcctcaaaa acaacaaata
     5581 caaaataatc tatataaaac aatttgttat acaatcagtt tcattccgat tc
//
LOCUS       DAOTRK010000220         5541 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.222, whole genome shotgun sequence.
ACCESSION   DAOTRK010000220 DAOTRK010000000
VERSION     DAOTRK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.222"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..108
                     /locus_tag="QIE41_004436"
     CDS             <1..108
                     /locus_tag="QIE41_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDS3533225.1"
                     /translation="SEITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
     gene            114..857
                     /locus_tag="QIE41_004437"
     CDS             114..857
                     /locus_tag="QIE41_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3533226.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTSEGERYFPCVNISGEPE
                     EYFRMSPEDWLRAEMQGEIVALVHSHPGGMPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            794..1402
                     /locus_tag="QIE41_004438"
     CDS             794..1402
                     /locus_tag="QIE41_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDS3533227.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGSEAIRALAMQIPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGLFQAVLGAALIAVA
                     WWNPAGWLGAAAITGMYGAGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDN
                     MVAQGNVLPVLYGEMRVGSRVISQEISTADEGDGGQVVVIGR"
     gene            1463..4942
                     /locus_tag="QIE41_004439"
     CDS             1463..4942
                     /locus_tag="QIE41_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDS3533228.1"
                     /translation="MGKGSSKGHIPREAKDNLKSSQMLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYETPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LAPGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKSHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGSVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGARHERENSDTYSSCRLI
                     VKKNGAEIYNRTALDNTLIYTGVIDMPAGSGVMTLEFSVSAWWVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            5010..>5541
                     /locus_tag="QIE41_004440"
     CDS             5010..>5541
                     /locus_tag="QIE41_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDS3533229.1"
                     /translation="MRKLCAVILSTVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     NDDLKGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSMMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDDRHSNTSLAW
                     GAGVQFNPTESVTIDLA"
BASE COUNT         1270 a         1371 c         1736 g         1164 t
ORIGIN      
        1 tccgaaatca cgaaggataa atgcagcaaa tgcctgagtg gctgtaagtt ccgcaataat
       61 gtcggcaact ttggcggctt cctttccatt aacaaacttt cgcagtaaat cccatgacag
      121 agacagaatc agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgcggct
      181 tcgtggtgag aacgtcggaa ggggaaagat attttccctg cgtgaatatc tccggtgagc
      241 cggaggagta tttccggatg tcgccggagg actggctgcg ggcagagatg cagggtgaga
      301 ttgtggcgct ggtccacagc caccccggtg gtatgccctg gctgagtgag accgaccggc
      361 ggctgcaggt gcagagtgat ttgccgtggt ggctggtttg ccggggagcg attcacaagt
      421 tccgctgtgt gccacatctt tccgggcggc gctttgagca cggggtgacg gactgttaca
      481 cgctgttccg ggatgcttac catctggcgg ggattgagat gccggatttt catcgcgagg
      541 atgactggtg gcgtaacggt cagaatctct atctggataa tctggaggcc acagggctgt
      601 atcaggtgcc gttgtcagcg gcgcagccgg gcgatgtgct gctgtgctgt tttggttcat
      661 cggtgccgaa tcatgccgcc atttactgtg gtgacagcga gctgctgcac catattcctg
      721 aacaactgag taaacgagag aggtacaccg acaaatggca gcgacgcaca cactccctct
      781 ggcgtcaccg ggcatggcac gcatctgcct ttacggggat ttacaacgat ttggccgccg
      841 catcgacctt cgagtgaaaa cggggtccga agccatccgg gcgctggcca tgcagatccc
      901 ggcgtttcgt cagaaactga gcgacggctg gtatcaggta cgcattgccg ggcgtgatgc
      961 aggtgaaact gaattatcag cccgtcttaa tgaaccgctg gcaaatggtg ccgtgatcca
     1021 cattgtgccg cgtctggcgg gagccaaaag tggcggtctt tttcaggctg tgctgggggc
     1081 ggcgctgatt gcggtggcat ggtggaaccc tgcaggttgg ttgggggctg ccgcaattac
     1141 aggaatgtat ggtgcggggg ccagtatggt gctcggtggt gtggcgcaga tgctggcacc
     1201 gaaagccaga actccccgta cacagacaac ggataacggc aaacagaaca cctatttctc
     1261 ctcactggat aacatggttg cccagggcaa tgttctgccg gttctgtacg gtgaaatgcg
     1321 cgtggggtca cgggtgattt cgcaggagat cagcacggca gatgaaggag atggtggtca
     1381 ggttgtggtg attggtcgtt gatgcagaat gttttgtgtg aaaccgcctc agggcggttt
     1441 tgtcgtttct ggagcgtgag gaatgggtaa aggcagcagt aaggggcata tcccgcgcga
     1501 agcgaaggac aacctgaaat catcccagat gctgagcgtg atagacgcca tcagtgaagg
     1561 gccgattgaa ggtccggtgg atggattaaa aagcgtgctg ctgaacagta cgccggtgct
     1621 ggacagtgag gggaatacca atatctccgg cgtcacggtg gtgttccggg caggtgagca
     1681 ggagcagtca ccgccggagg gatttgaatc ctccggttcc gagacggtgc tgggtacgga
     1741 agtgaaatat gaaacgccga tcacccggac catcacgtcg gcaaacatcg accgtctgcg
     1801 ctttaccttc ggtgtacagg cactggtgga aaccacctca aagggagaca ggaatccgtc
     1861 ggaagtccgc ctgctggttc agatacagcg taacggtggc tgggtgacgg aaaaagacat
     1921 caccattaag ggcaaaacca cctcgcagta tctggcctcg gtggtggtgg ataacctgcc
     1981 gccgcgcccg ttcaatatcc ggatgcgcag gatgacgccg gacagcacca cagaccagct
     2041 gcagaacaaa acgctctggt cgtcatacac cgagatcatc gatgtgaaac agtgctaccc
     2101 gaacacggca ctggtcggcg tacaggtgga ctcggagcag ttcggcagcc agcaggtgag
     2161 ccgtaattat catcttcgcg ggcgcattct gcaggtgccg tcgaactata acccgcagac
     2221 gcgacaatac agcggtatct gggacggaac gttaaaacca gcatacagca acaacatggc
     2281 ctggtgtctg tgggatatgc tgacccaccc gcgctacggc atggggaaac gtcttggtgc
     2341 ggcggatgtg gacaaatggg cgctgtatgt catcggccag tactgcgacc agtcagtgcc
     2401 ggacggcttt ggcggcacgg agccgcgcat cacctgtaat gcgtacctga ccacacagcg
     2461 caaggcgtgg gatgtgctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa
     2521 cgggcagacg ctgacgttcg tgcaggaccg acagtcggat aaggtgtgga cctataaccg
     2581 cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc tacagcttca gcgccctgaa
     2641 ggaccgccat aatgccgttg aggtgaactg gattgacccg aacaacggct gggagacggc
     2701 gacagagctt gttgaagata cgcaggccat tgcccgttac ggtcgtaatg tcacgaagat
     2761 ggatgccttt ggctgtacca gccgggggca ggcacaccgc gccgggctgt ggctgattaa
     2821 aacggaactg ctggaaacgc agaccgtgga tttcagcgtg ggcgcagaag ggcttcgcca
     2881 tgtgccgggc gatgttattg aaatctgcga tgatgactat gccggtatca gcaccggtgg
     2941 tcgcgtgttg gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcacgct
     3001 gccatcctcc ggtaccacgc tgataagcct ggttgacgga agtggcaatc cggtcagcgt
     3061 ggaggttcag tccgtcaccg acggacttaa ggtgaaagtg aatcgggttc ctgacggcgt
     3121 tgcagaatac agtgtgtggg ggctgaagtt gccgacgttg cgtcagcgcc tgttccgctg
     3181 tgtgagtatc cgtgagaacg atgacggcac gtatgccatc actgccgtgc agcatgtacc
     3241 ggaaaaagaa gccatcgtgg ataacggggc gcactttgac ggcgaccaga gcggcacggt
     3301 gaatggtgtc acgccgccag cggtgcagca cctgaccgcc gaagtcaccg cagacagcgg
     3361 ggaataccag gtgctggcgc gctgggatac gccgaaggtg gtgaagggcg tgagctttat
     3421 gcttcgcctg accgtggcag cggatgacgg cagtgagcgg ctggtcagca cggcccggac
     3481 gacggaaacc acataccgct tcacgcaact ggcgccaggg aactacaggc tgacagtccg
     3541 ggcggcaaat gcctgggggc agcagggcga tccggcatcg gtatcgttcc gtattgccgc
     3601 accggcagcg ccgtcgcgga ttgagctgac gccgggctat tttcagataa ccgccacgcc
     3661 ccatcttgcc gtttatgatc cgacggtaca gtttgagttc tggttctcgg aaaagcggat
     3721 tgcggatatc aggcaggttg aaaccacagc ccgctatctt ggcacggcgc tgtactggat
     3781 agctgccagt atcaatatca aaccgggcca tgattattat ttttacatcc gcagtgtgaa
     3841 caccgttggc aaatcggcat ttgtggaggc tgttggccag ccgagtgatg atgcatccgg
     3901 ctatctggat tttttcaaag gcgagatagg gaaaagccat ctggctcagg agctgtggac
     3961 gcagattgat aacggtcagc ttgcgcctga cctggctgaa atcaggacgt ccattacgga
     4021 tgtcagcaat gaaatcacgc agaccgtcaa taagaaactg gaagaccaga gtgcggcaat
     4081 tcagcagata cagaaggttc aggttgatac aaataataac ctgaacagca tgtgggctgt
     4141 gaagctgcag cagatgcagg acggacgcct ttatatcgcg ggtattggtg ccggtattga
     4201 gaacacccct gacggcatgc agagtcaggt gctgctggcg gcggacagga ttgcgatggt
     4261 taatcctgcg aatggcaaca caaagccgat gtttgttggt cagggcgatc agatattcat
     4321 gaacgaagtg ttcctgaaat atctgacggc tcccaccatt accagcggcg gtaatcctcc
     4381 ggcattttcc ctgacaccgg acgggcggct gacggcgaaa aatgccgata tcagcggtag
     4441 cgtgaatgcg aactccggga cgctcaacaa cgtcacgatt aacgagaact gtcgggttct
     4501 gggaaaactg tccgcgaacc agattgaagg cgatctcgtt aaaacagtgg gcaaagcttt
     4561 cccccgggac tcccgtgcac cggagcggtg gccatcaggg accattaccg tcagggttta
     4621 tgacgatcag ccgtttgacc ggcaaattgt tattccagcg gtggctttca gcggtgccag
     4681 acatgaacgg gagaatagcg atacttattc gtcatgccgc ctgatagtga agaaaaacgg
     4741 ggctgaaatt tataaccgaa cggctctgga taatactctg atttacacgg gtgttattga
     4801 tatgcctgca ggcagtggcg taatgacact ggagttttct gtatcagcat ggtgggtaaa
     4861 tggctggtat cccacagcaa gtatcagcga tttgctggtt gttgtgatga agaaagccac
     4921 tgcaggcatc acgattagct gaattttata acccagatac gggcgccaga aatggcgcct
     4981 tttttattgc agaaaagcga gaggtaatta tgcgtaaatt atgtgctgtt attctgtcca
     5041 cagtagtctg gctggtcgcc gctggtacgc cagcgagcgc agcagagcat cagtccacac
     5101 taagcgccgg gtatcttcag acccatactg atatgcccgg caacgatgac ctgaagggca
     5161 ttaacgtgaa ataccgttat gaatttacgg acacgctggg gctggtgacg tcattcagtt
     5221 atgccaatgc caaagatgag caaaaaacgc attacagcga tacccgctgg catgaagatt
     5281 cagtgcgtaa ccgctggttc agcatgatgg cggggccgtc tgtacgcgtg aatgaatggt
     5341 tcagtgctta tgcgatggca ggtgtggctt acagccgtgt ttcgacgttc tccggggatt
     5401 atctccgcgt aactgacaac aaggggaaaa cgcacgatgt gctgaccgga agtgatgacg
     5461 atcgccacag caacacgtct ctggcgtggg gagctggcgt gcagtttaac ccgaccgaat
     5521 ccgtgaccat tgaccttgct t
//
LOCUS       DAOTRK010000221         5502 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.223, whole genome shotgun sequence.
ACCESSION   DAOTRK010000221 DAOTRK010000000
VERSION     DAOTRK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.223"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1..491
                     /locus_tag="QIE41_004441"
                     /pseudo
     CDS             1..491
                     /locus_tag="QIE41_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(554..1114)
                     /locus_tag="QIE41_004442"
     CDS             complement(554..1114)
                     /locus_tag="QIE41_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3533230.1"
                     /translation="MIKNTRYKIVLLAGIFLLPGALAQDINVDFVATIKGTTCNITLE
                     GTRVTADGNNQYTLRILNVALDKIINKMPEAQADFKLVANCDGNIGKITTSLAGNPSV
                     ELPYLISPLTSDNSSTTEYIAMGIKLRNASDTEFIPPDGSKKISWGEGLTKPELEMTV
                     ALRETRPGSGRAGSFKALATFNFTYE"
     gene            complement(1133..3733)
                     /locus_tag="QIE41_004443"
     CDS             complement(1133..3733)
                     /locus_tag="QIE41_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HDS3533231.1"
                     /translation="MKTTTSTKRHNLTRIAMYCSIMYSGATIGAESVEYDPTFLMGGN
                     ASSIDVSRYSDGNPTLPGVYDVSIYVNEQPVANLEIPFIAIPDKKNAQACITLKNLLQ
                     LHIKTPPADEENTILLPRDETLGNCLDLSLAIPKSSVNYDPSEQRLDINVPQAWVMKN
                     YQNYVDPSLWENGINAATLSYNINAYRSENSNYTNDSVYTSFNGGVNLGAWRLRSSGN
                     YSWRNDAGSNVEFMNRYVQRDITAIRSQLIMGESYTTGETFDSVSIRGIRLYSDSRML
                     PPVLANFAPTIRGVANTNAKVSITQSGYKIYETTVPPGPFVIDDLSPSGYGSDLIVTI
                     EEADGSKRTFSQPFSSVIQMLRPGVSRWDISGGQINKDDLHHEPNLLQATYYRGLSNL
                     FTGYTGFQVTDNHYAAGLLGIGMNTSVGAISFDVTHSSVDIPDDKRYQGQSYRISWNK
                     FFDLTDTSLNIAAYRYSTQDYLGLNDALTLIDEVEHPTQDLDPKTMRNYGRMKNQFTV
                     SINQPLRFGKDDYGSFYTSGSWSDYWGGSKSRSNYSVGYSNSASWGSYSISAQRSWDE
                     YSQTENSIYLSFSIPIEKLMGTEHRDSGFQSIDTQLSTNMDGNNQFNMSSSGYSNDNR
                     VSYSVNAGYGMNKTGKDLSNIGGYASYESPWGTLAGSVSATSDNNRQYSVNTDGGFVL
                     HSGGLTFSNDSFSDNDTIALVKAPGAKGARINYGNNTVDRWGYGVTSALSPYQENKIA
                     LDTENLENDIEMKSTSTVAVPRQGSVIFAGFETNQGQSAIMNIKRSDGKGIPFAADIH
                     DETNAIIGNVGQGGQAFVRGIQQQGTIKITWLEASMPKTCIAQYQQPNVTSEKIQQTI
                     ILNNILCQLQ"
     gene            complement(3768..4502)
                     /locus_tag="QIE41_004444"
     CDS             complement(3768..4502)
                     /locus_tag="QIE41_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDS3533232.1"
                     /translation="MLLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVS
                     VRLENKGNSHLLVQSWLDTGDDNAEPGSINVPFTATPPVSRIDAKRGQTIKLMYTGST
                     VLPSDKESVFWFNVLEVPPKPDATKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPL
                     ALKWKWAEGKSGLSVYNPTPYYVSFNSAELIAGGKSNPLNVKMVAPKSEEIFTISGSG
                     GKATSGKVHFSAINDFGGAIEGDVNL"
     gene            complement(4592..5164)
                     /locus_tag="QIE41_004445"
     CDS             complement(4592..5164)
                     /locus_tag="QIE41_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3533233.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQINISGLVSDNTCETK
                     VDGGKQDGLVLLQTATVAEITAGVSETAVGAKAKPFSITVDCSKATPGGQPTLTFGSV
                     FFGNSRGTLNNDMSITTPSSGVNIALHNIDGTTVKQVKINNASDAYTKALTNNVAVFD
                     FKASYVREDDSTPATAGYVRTNTAYTITYN"
BASE COUNT         1411 a         1216 c         1277 g         1598 t
ORIGIN      
        1 gcccgcatta tgggcgttgg cctcaacacg attttccgcc atttaaaaaa ctcaggccgc
       61 agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg
      121 aacagtgggg atacgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca
      181 ggctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactctggcc acactggagc
      241 gtcttctaag cctgctgtcg gcctttgagg tcgtggtatg gatgacggat ggctggccgc
      301 tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgttac actcagcgaa
      361 ttgagcggca taacctgaat ctgagacaac atctggcaag gctgggacgg aagtcactgt
      421 cgttctcaaa atcggtggag ctgcatgaca aggtcatcgg gcattatctg aacataaaac
      481 actatcagta agttggagtc attacccaaa tatcccataa tgtaatgatt tcatctgcat
      541 tattattatc cttttattcg taggtgaaat tgaatgtcgc aagtgcttta aaactacctg
      601 ctcttcctga acctggtcgg gtttcgcgaa gtgctaccgt catctcaagt tccggttttg
      661 ttaatccctc tccccaggaa atctttttag aaccatctgg gggaataaat tcagtgtcag
      721 aggcatttcg aagtttaatc cccatagcaa tatattctgt ggtggatgaa ttatctgaag
      781 ttaatggaga aattaaatag ggtaactcta ctgaaggatt ccctgccagc gatgtcgtaa
      841 tcttgcctat gttaccatca caattagcga ccagtttaaa gtcggcttgt gcttctggca
      901 tcttgtttat gattttatcc agagcaacat ttaatatccg tagcgtgtac tgattattgc
      961 catcagcggt gacgcgtgtc ccttccagag tgatattaca tgtcgtgcct ttgattgtgg
     1021 caacaaaatc aacattaata tcttgtgcca gtgcgccggg cagtaaaaaa atgcctgcca
     1081 ggagtactat tttataccgt gtgtttttta tcattgtcat ctctctttcg tattattgga
     1141 gctggcaaag aatattattg agaataatcg tttgctggat tttctccgat gtcacattgg
     1201 gttgctggta ctgcgcaata caggttttgg gcatgctggc ttccagccag gtgattttta
     1261 tcgtgccctg ctgctgaata ccgcgaacaa atgcctgccc gccctgccca acgttgccaa
     1321 tgattgcatt tgtttcatca tgaatatcgg cggcgaaggg gatacctttt ccgtcactgc
     1381 gtttgatatt cataatcgca gattgtccct gattggtttc aaaaccagca aagataaccg
     1441 agccctggcg tggtacggca accgtactgg tgctcttcat ttcgatgtcg ttttccaggt
     1501 tttcagtgtc cagagcaatt ttgttttcct ggtatggtga aagagcactg gttacgccat
     1561 atccccaacg gtcaacggta ttgttgccgt agttaatacg cgcccctttt gcacccggtg
     1621 ctttaaccag agcaatggtg tcattatcac tgaaactgtc gttgctgaac gtcagtcctc
     1681 cgctgtgcaa cacaaatcca ccatctgtat taacggagta ctggcggtta ttatcgctgg
     1741 tagcagagac tgacccagct agcgttcccc atggcgattc atagctggca taacccccaa
     1801 tgttgctgag atctttgccg gttttattca tcccataacc cgcgttcacg ctatagctca
     1861 cgcggttatc gttgctataa ccgctactgc tcatgttgaa ctgattatta ccatccatat
     1921 tggtactaag ttgtgtatca atactttgga aaccggaatc acggtgctcc gtacccatca
     1981 gcttttcgat aggtatcgaa aagctgaggt aaatactgtt ttctgtttgg ctgtactcat
     2041 cccaggagcg ttgagcgctg atgctgtagc ttccccatga agcgctgtta ctgtagccga
     2101 ccgaatagtt gctacggctc ttactgcccc cccagtaatc agaccagctt ccagatgtat
     2161 agaacgagcc ataatcatcc ttcccaaagc gtagcggttg gttaatgctt acggtgaact
     2221 ggtttttcat gcgcccgtag ttgcgcatag ttttgggatc aagatcctga gtgggatgtt
     2281 caacttcatc aataagtgtt aatgcatcat tcaggcccag atagtcctgc gtggagtagc
     2341 ggtaagccgc gatgtttaat gatgtatctg tcaggtcaaa aaatttgttc caggagatgc
     2401 gatagctttg cccctggtac cttttatcat ccggtatatc gacactggag tgggtgacat
     2461 cgaaggaaat tgcgccgaca gaagtattca tcccaatgcc aagtaagcct gcagcataat
     2521 gattatctgt gacctgaaaa ccagtatagc cagtgaaaag gttacttaaa ccacggtaat
     2581 aggtcgcttg tagtaagttt ggttcatgat gcaagtcgtc tttattaatt tgcccgccgc
     2641 tgatatccca gcgagaaaca cccgggcgca gcatttgaat tactgaagaa aatggctgag
     2701 aaaatgtacg cttgctgcca tcagcttctt caatggttac gataagatcg ctgccatacc
     2761 ctgacgggct gagatcatca atgacaaaag gtcccggtgg aacagtggtt tcgtaaattt
     2821 tgtatccact ctgggtgatg ctgactttgg cgttagtatt tgctacacca cgaattgtcg
     2881 gggcgaagtt tgccaataca ggcggcagca ttcggctgtc gctgtataag cgaatgccac
     2941 ggatactaac ggagtcgaac gtttcacctg tggtgtagga ttcccccata atgagctgcg
     3001 agcgaatagc ggtaatgtcg cgctggacgt agcgattcat aaactcaaca ttgcttcccg
     3061 cgtcgtttct ccagctatag ttaccgctgc tacgcagacg ccaggcccct aaattgacgc
     3121 caccattgaa cgatgtataa acgctatcat ttgtataatt tgaattttca ctgcgataag
     3181 cgttgatgtt ataagacagc gtagccgcgt taatgccatt ttcccaaagt gatgggtcca
     3241 catagttttg ataattcttc atcacccatg cctggggaac attgatatcc agacgttgtt
     3301 cagaggggtc atagttcact gaggatttag gtatggctaa cgacaggtca agacagttgc
     3361 caagcgtttc atcccgtgga agaaggatag tattttcctc gtctgccggg ggtgtcttga
     3421 tatgcagttg tagtagattt ttcagggtaa tacaggcttg agcattcttt ttatcgggga
     3481 tcgcgataaa ggggatctcc aggttagcaa caggttgctc gttaacatag atgctaacat
     3541 cgtaaacacc aggcaaagtc gggttgccat cgctatagcg gctaacatca atactggaag
     3601 cgttgccacc cattaaaaag gtagggtcat actcaacgct ttcggctccg atggtagcgc
     3661 cgctatacat aattgagcaa tacattgcga tacgcgtaag attatggcgt ttagtcgatg
     3721 tcgttgtttt cattacattg gcttcctgct gcataaggtc cctggtctta caaattgaca
     3781 tcgccttcaa ttgcgccgcc aaaatcatta atcgcggaga aatgtacctt tcctgacgtt
     3841 gctttcccgc ctgaaccgct tatggtgaaa atctcttcgc ttttcggtgc aaccattttt
     3901 acattgagcg ggttcgactt accgccagcg attaactctg cactgttgaa agagacgtaa
     3961 taaggtgttg ggttatatac actcaggcca gacttccctt cagcccattt ccatttcagg
     4021 gctaatggtg cttcagacgg gttaccgttt aatcctgccg gacgatagaa cagcttgata
     4081 cgggtacgga aagccagttg taacaggctc tgattctcta cttttgtagc atctggtttt
     4141 gggggcactt ccagcacgtt aaaccagaat acgctttctt tatcgctggg gagtacggta
     4201 ctgccggtat acatcaattt gatggtttga ccgcgtttag catcaatacg tgaaacggga
     4261 ggtgttgccg taaatggtac attaatgctt cctggctcag cgttatcgtc accggtatcc
     4321 agccagcttt gtactaacag gtggctattg cctttattct ccaggcgtac gctaacgctt
     4381 ttttgatcac ttttataaat gacgcgtgta ccggaaataa caatatcagc cataacaggc
     4441 atgctaaatg ccatactact ggcaacacat aatgcgacaa tattcttttt atttaacaaa
     4501 atataccatc catgacatga attgttttca agacaagggg gcgacaatag gtgtcgctcc
     4561 cttgtagtaa tttaaatcac caacgaataa cttaattgta ggtgatggtg taagctgtgt
     4621 tggttcttac ataaccagcc gttgctggtg ttgagtcgtc ttcacgaaca taggaggctt
     4681 tgaagtcaaa gactgcaaca ttattggtta atgctttagt gtaagcatca gatgcattat
     4741 taattttgac ttgtttaaca gtggtgccgt caatgttgtg cagggcaata ttcacaccag
     4801 atgacggggt ggtgatggac atatcgttat tcaaagtgcc acgactgtta ccaaagaaaa
     4861 ctgaaccgaa tgtcagagtc ggttggcctc ccggagtcgc tttgctgcag tctacagtaa
     4921 tgctaaacgg tttcgctttg gcaccgacag cagtttcaga tacaccagcg gtaatttcag
     4981 caaccgttgc agtttgcaga agaaccaggc catcttgctt gccgccatca acttttgttt
     5041 cacaggtgtt atcgctaacc aagccactga tgttaatctg accaccgtcc atatcagcag
     5101 aagcagtacc tgcaaccatt gccagcataa gaccagacag ggcaaaacct aattttttag
     5161 acataataat ccttgaatta tattccatga gtttaactac aatcgggtgc tttcagacaa
     5221 agaaccccga tagagaaaaa tagattggtt tgttaatgat gaatgtgaag atttactgaa
     5281 tgtaacatct gttcatcacc aagtgggata ttgtgcatat ttaaatttat atatccactt
     5341 aattcatctt taagtaaaac ttaatgtaca aagcgggaaa aatattgtat gtgaggcgga
     5401 aggtgttaac ggggtatata atattaatta tggttgtagc ttagggaata tattaattgc
     5461 aatggggtaa ggctattttt cggaatttat ttatattcat ga
//
LOCUS       DAOTRK010000222         5448 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.224, whole genome shotgun sequence.
ACCESSION   DAOTRK010000222 DAOTRK010000000
VERSION     DAOTRK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.224"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..564
                     /gene="csgD"
                     /locus_tag="QIE41_004446"
     CDS             <1..564
                     /gene="csgD"
                     /locus_tag="QIE41_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDS3533234.1"
                     /translation="LKQSLAITGKLHNIQRSLDDISSGSIILLDMMEADKKLIHYWQD
                     TLSRKNNNIKILLLNTPEDYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYF
                     TQKLASYLITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKT
                     HLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            569..958
                     /gene="csgE"
                     /locus_tag="QIE41_004447"
     CDS             569..958
                     /gene="csgE"
                     /locus_tag="QIE41_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDS3533235.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            983..1399
                     /gene="csgF"
                     /locus_tag="QIE41_004448"
     CDS             983..1399
                     /gene="csgF"
                     /locus_tag="QIE41_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDS3533236.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1426..2259
                     /gene="csgG"
                     /locus_tag="QIE41_004449"
     CDS             1426..2259
                     /gene="csgG"
                     /locus_tag="QIE41_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDS3533237.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2323..2814)
                     /locus_tag="QIE41_004450"
     CDS             complement(2323..2814)
                     /locus_tag="QIE41_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDS3533238.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLFS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAVRSTKTAHREQEIK
                     NKA"
     gene            complement(2916..3470)
                     /gene="ycdY"
                     /locus_tag="QIE41_004451"
     CDS             complement(2916..3470)
                     /gene="ycdY"
                     /locus_tag="QIE41_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDS3533239.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3494..4231)
                     /gene="ycdX"
                     /locus_tag="QIE41_004452"
     CDS             complement(3494..4231)
                     /gene="ycdX"
                     /locus_tag="QIE41_004452"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDS3533240.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4286..5224)
                     /gene="ghrA"
                     /locus_tag="QIE41_004453"
     CDS             complement(4286..5224)
                     /gene="ghrA"
                     /locus_tag="QIE41_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDS3533241.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWLHPRVAITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1482 a         1363 c         1259 g         1344 t
ORIGIN      
        1 cttaaacaat cgctggcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
       61 atatcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
      121 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
      181 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
      241 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg cggcgaatgc
      301 tactttacgc aaaagcttgc cagttacctg attacgcatt caggtaacta tcgttataac
      361 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
      421 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
      481 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
      541 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
      601 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
      661 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
      721 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
      781 ctggatcact ataacggtca atcaggacgt tattttccag acttttttat tcccgttgaa
      841 aagagacttc gaaaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
      901 tcgccagata aatcaggcgt tattaagtac gggcgatttg gcgcatgatg aattctaaat
      961 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     1021 ttcgccatta agttgggctg gaaccatgac ttttcagttc cgtaatccaa actttggtgg
     1081 taacccaaat aatggcgctt ttttattaaa tagcgcccag gcacaaaact cgtataaaga
     1141 tccgagctat aacgatgact atggtattga aacaccctca gcgttagata actttactca
     1201 ggccatccag tcacaaattt taggtgggct actgtcgaat attaataccg gtaaaccggg
     1261 ccgcatggtg accaacgatt atattgttga tattgctaac cgcgatggtc aattgcagtt
     1321 gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa
     1381 taactcaacc gatttttaag ccccagcttc ataaggaaaa taatcatgca gcgcttattt
     1441 cttttggttg ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa agaagccgcc
     1501 agaccgacat taatgcctcg tgctcagagc tacaaagatt tgacccatct gccagcgccg
     1561 acgggtaaga tctttgtttc ggtatacaac attcaggacg aaaccgggca atttaaaccc
     1621 tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg ccacggcaat gctggtcacg
     1681 gcattgaaag attctcgctg gtttataccg ctggagcgcc agggcttaca aaacctgctt
     1741 aacgagcgca agattattcg tgcggcacaa gaaaacggta cggttgccat taataaccga
     1801 atcccgctgc agtctttaac ggcggcaaat atcatggttg aaggttcgat tatcggttat
     1861 gaaagcaacg ttaaatcggg cggtgttggg gcaagatatt ttgggatcgg tgccgacacg
     1921 caataccagc tcgatcagat tgccgtgaac ctgcgcgtcg tcaatgtgag taccggtgag
     1981 atcctctcct cggtgaacac cagtaagacg atactttcct atgaagttca ggcgggggtc
     2041 ttccgcttta ttgactacca gcgcttgctg gaaggggaag taggttacac ctcgaacgaa
     2101 cccgttatgc tgtgcctgat gtcggctatc gaaacaggcg tcattttcct gattaatgat
     2161 ggtatcgacc gcggtctttg ggatttgcag aacaaagcag aacggcagaa tgacattctg
     2221 gtgaaatacc gccatatgtc ggttccaccg gaatcctgat agataaaaat gccggaatta
     2281 tcatccggca ttttactgtt taagcgaaag cgtgagtgcc cctcacgctt tatttttgat
     2341 ttcctgctca cggtgagcgg tcttcgtact acgcaccgcc agccaaaggc cgctgttttt
     2401 cattgcgtaa ccaaatatca acccgagcgc cagcgaaggt aacaccagtt tccagtcacc
     2461 ctgcccggca aacgtcgcgc atgcgcctat aaacgttccc ggaacaaatg aaaacagcag
     2521 ctgtttggcc tgaatacaca tcagaaacgc gacaataccg gttatgacat aaccgagaat
     2581 ttccagatgt ggtgccagtg cactaccgta aataatgacc atcgcccaca ccacgccact
     2641 aagcagggtt gcagcggaga tcgccagccc tttcagccca ccttgcgggc aggcaaagta
     2701 ggccgtacag cccaggaagc ccgcccagct tagtaagccc agggaaacag ccacccatcc
     2761 ccagataccg gagagaatgc ctgttgtgat tgcaatagag agaagtatgt tcatgccgcg
     2821 catgatagca gaaaacgcgg ccatgaatga gatcgagcac acattttatg caacaacaaa
     2881 ttatctgttg cagttatgag tgatgcagat cacatttact cttcagaatc ttcttccagc
     2941 tcgtcccaca ttgcactaat agcatcgcgg gttagcggtg ccatggtgcg ccagaaaggc
     3001 gtagttgcat gggcctccac tttgccaagg aacgcaccac accagggtaa cagatactca
     3061 ctgaacagtg tttccagtgc ttcgctctca tcttccgttg actgatcttc cagccaggaa
     3121 gccgcgagca gcaatgtgcc gatgtgatcg gctggcgtat ccgctaatgg catcccccgt
     3181 tcggaaagaa aagcgcgcac ttccgcttcc gtcgcgccct caacccatgc gctacgatat
     3241 ggcggcacag cacattcatc gccgataaac aacgcattgt aatcggcaga gacttgcgcc
     3301 atatcacagc ttttctgtaa acgcgtcagt aactcatcct gctccagtgg ccagttcgca
     3361 gccagtttcc cctcacgaat cagggtaaac agcggcacca gtaaaggatc ttgcggttgg
     3421 cggtaataga gcgaacccag cacacgacag aggatagaaa actcgttcat taatatattt
     3481 ccattactag taattaaagg tctgcaaatt ccgcaatcgg tgccataccg cgagattcaa
     3541 ggaagttcag taagcgccgc ggagaaacat tcaaaatgcg ctctggcgga aaatctaccg
     3601 cgtcgaggat tttaagacac tcttcaaatt cacccatggt aaacgcggtg tgagaatccg
     3661 agcctaatgc cacccaacct ccagcgtcac gtaccgctgc ggctaccgcc cgacagttgt
     3721 cttcactgcc cttacgtgag tgtaaaaatg aggaattatt gatttccagc gccacctgat
     3781 gtttcgcagc tgcttcagca acggctttaa catctatttc atatttcgga tttccgggat
     3841 ggcttattat atgcacattg ccgctggcga tagtggcgat catcgcttgc gtattagtcg
     3901 ctttgtcatg cggcgcaaac accggctcat gaaaaccggc aataatcaga tccagcgaat
     3961 caaacatttt gccactgcag tcgatttcac cgtcaacatt tttaatgtta gcttcgatgc
     4021 cgcgcaggat cccaacccca tcaaccactc gcggccagat acgcatgtta atgaagtgcc
     4081 agtgatgcgg cgcatcttcc atatccgggc catgatcggt gatcgcaaaa agtttaatgc
     4141 ccttttgttt ggcctgggca atgtaatcac tcaatgtgct atatgcatgc gtgctggcaa
     4201 cggtatgcat atgaaggtcg acgggataca taactctctc ctgtattcaa ttcccgccaa
     4261 ggatagcagg aaacctgatg ctttattagt agccgcgtgc gcggtcgact tgcccgcaga
     4321 ccctctcccc tttttcgagc tgggcaatgg tgcgagaaat gtactccaca gcttcagcgg
     4381 gacgggtaat cgcggcgaca tgtggtgtta tcgccacgcg aggatgtagc cagagcggac
     4441 tttccggcgg taagggttcg cgattaaaaa catccaacat tgcgccttta actttcccgc
     4501 tatccagcgc cgcgagcagg tcatcttcca cgacatgaac accacgcgcc aggttgagga
     4561 gatacgcgcc atccggtaat ttttcgagta attgttgatt aataatgccg acggtttcag
     4621 gggtattcgg taacagatta atcaataccc ggcattggct cagaaatgca gaaagttctt
     4681 cccgtccggc aaaactttgc acgccaggcc acgatttacg ggttcgactc cagcaacgca
     4741 gcgggaagcg ccaggtttgc agactctgtg caactttact gcccagtacg cctgcgccca
     4801 aaatgccgat ggtaaaatct tcccgatgat attcaggaag cggttgccaa tgcgaactat
     4861 tttgctggat gcgataatcg tcaaaacgtc gaaaccaatg cagcacctga ctgacagcat
     4921 attcctgcat ttgctcgccc ataccggtat cttccaggcg aaaaagtgga acagaaggct
     4981 tcagcatttc agggtgtgcc tgtagcttgc tcaaaataga atcaacaccg gccccgagtg
     5041 cgaacaccgc tttaagatcg cgccctgcca gcatttcaac tggaggatgc cagactaaag
     5101 cataatcagc agagtcatta tctccgcttt tccatgctct gactcttgcc tgaggaatag
     5161 ctttgcgcag tgcctcaatc caccattggg tatcgaacgt tgggtgataa aagatgatat
     5221 ccatactgac tcccgaaaag cgttgcgcga aatttattcg cacttatcgt tatgatctac
     5281 aaaagccacc agcataacaa atccgtggtc ggtggcaaaa aaagcagatt tcgcttatta
     5341 aaactgcata ttgattgaag tttgaataaa cgcgcgattt tttaaaaaag tttgttgacc
     5401 tcaggtcatg atttccctaa attagcgccc gttccagcaa gacaggaa
//
LOCUS       DAOTRK010000223         5430 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.225, whole genome shotgun sequence.
ACCESSION   DAOTRK010000223 DAOTRK010000000
VERSION     DAOTRK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.225"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            36..1337
                     /gene="dcuA"
                     /locus_tag="QIE41_004454"
     CDS             36..1337
                     /gene="dcuA"
                     /locus_tag="QIE41_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDS3533242.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            1452..1790
                     /gene="cutA"
                     /locus_tag="QIE41_004455"
     CDS             1452..1790
                     /gene="cutA"
                     /locus_tag="QIE41_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDS3533243.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            1766..3463
                     /gene="dsbD"
                     /locus_tag="QIE41_004456"
     CDS             1766..3463
                     /gene="dsbD"
                     /locus_tag="QIE41_004456"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDS3533244.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVLADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQVRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMLLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            3500..4075
                     /locus_tag="QIE41_004457"
     CDS             3500..4075
                     /locus_tag="QIE41_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS3533245.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            4182..4257
                     /locus_tag="QIE41_004458"
     tRNA            4182..4257
                     /locus_tag="QIE41_004458"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4215..4217,aa:Phe,seq:gaa)
     gene            4455..>5430
                     /locus_tag="QIE41_004459"
     CDS             4455..>5430
                     /locus_tag="QIE41_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDS3533246.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEVKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNK"
BASE COUNT         1247 a         1451 c         1369 g         1363 t
ORIGIN      
        1 taatatcaac ttgttaaaaa acaaggaagg ctaatatgct agttgtagaa ctcatcatag
       61 ttttgctggc gatcttcttg ggcgccagat tggggggaat aggtattggt tttgcaggcg
      121 gattgggggt gctggttctt gccgctattg gcgttaaacc cggtaacatc ccgttcgatg
      181 tcatttccat tatcatggcg gttatcgccg ctatttctgc catgcaggtt gctggcggtc
      241 tggactatct ggttcatcag acagaaaagc tgctgcgccg taacccgaaa tacatcacga
      301 tcctcgcacc gatcgtgacc tatttcctga caatctttgc tggtactggc aacatctctc
      361 tggcgacact gccagttatc gctgaagttg cgaaggaaca aggcgttaaa ccttgccgtc
      421 cgctgtctac tgcagtggta tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg
      481 cagtggttta catgtcttcc gtaatggaag gtcatggcat cagctacctc catctgctct
      541 ccgtggtcat cccgtccacc ctgctggcgg ttctggtgat gtccttcctg gtcactatgc
      601 tgttcaactc caaactctct gacgatccga tttatcgcaa gcgtctggaa gagggcctgg
      661 ttgaactgcg cggtgaaaag cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc
      721 tgttcctgct gggcgtagtt ggcgttgtta tctatgcaat catcaacagc ccaagcatgg
      781 gtctggttga aaaaccgctg atgaacacca ccaacgcaat cctgatcatc atgctcagcg
      841 ttgcaactct gaccaccgtt atctgtaaag tcgataccga caacatcctc aactccagca
      901 ccttcaaagc aggtatgagc gcctgtattt gtatcctggg tgttgcatgg ctgggcgata
      961 ctttcgtttc caacaacatc gactggatca aagataccgc tggtgaagtg attcagggtc
     1021 atccgtggct gctggccgtc atcttcttct ttgcgtctgc tctgctgtac tctcaggctg
     1081 caaccgcaaa agcactgatg ccgatggctc tggcactgaa cgtttctccg ctgaccgctg
     1141 ttgcttcttt cgctgcggtg tctggtctgt tcattctgcc gacctacccg acgctggttg
     1201 ctgcggtaca gatggatgac acgggtacta cccgtatcgg taaattcgtc ttcaaccatc
     1261 cgttcttcat cccgggtact ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg
     1321 gtagcttcat gctgtaatga ccaatcgcgg ggcttcaggc cccgctttct ttcccgccga
     1381 ctaacatcct tcccccgtcc gttgtatagt gacctctctc ttgcggttcc atctgttctt
     1441 gcgaggtgtt tatgcttgat gaaaaaagtt cgaataccgc gtctgtcgtg gtgctatgta
     1501 cggcaccgga tgaagcgaca gcccaggatt tagccgccaa agtgctggcg gaaaaactgg
     1561 cggcctgcgc gaccttgatc cccggcgcta cctctctcta ttactgggaa ggtaagctgg
     1621 agcaagaata cgaagtgcag atgattttaa aaactaccgt atctcaccag caggcactgc
     1681 tggaatgcct gaagtctcat catccatatc aaaccccgga acttctggtt ttacctgtta
     1741 cacacggaga cacagattac ctctcatggc tcaacgcatc tttacgctga tcctgctact
     1801 ttgcagcact tccgtttttg ccggattatt cgacgcgccg ggacgttcac aatttgtcct
     1861 cgcggatcaa gcctttgctt ttgattttca gcaaaaccaa catgacctga atctgacctg
     1921 gcagatcaaa gacggttact acctctaccg taaacaggtc cgcattacgc cggaacacgc
     1981 gaaaattgcc gacgtgcagc tgccgcaagg cgtctggcat gaagatgagt tttacggcaa
     2041 aagcgagatt taccgcgatc ggctgacgct tcccgtaacc atcaaccagg cgagtgcggg
     2101 agcgacgtta actgtcacct accagggctg tgctgatgcc ggtttctgtt atccgccaga
     2161 aaccaaaacc gttccgttaa gcgaagtggt cgccaacaac gcagcgtcac agcctgtgtc
     2221 tgttccgcag caagagcagc ccaccgcgca attgcccttt tccgcgctct gggcgttgtt
     2281 gatcggtatt ggtatcgcct ttacgccatg cgtgctgcca atgtacccac tgatttctgg
     2341 catcgtgctg ggcggtaaac agcggctttc cactgccaga gcattgttgc tgacctttat
     2401 ttatgtgcag gggatggcgc tgacttacac ggcactgggt ctggtggttg ccgccgcagg
     2461 gttacagttc caggcggcgc tacagcaccc atacgtgctc attggcctcg ctatcgtctt
     2521 taccttgctg gcgatgtcaa tgtttggctt gtttaccctg caactcccct cttcgctgca
     2581 aacgcgcctc acgctgatga gcaatcgcca acagggcggc tcacctggcg gtgtgtttgt
     2641 tatgggggcg attgccggac tgatctgttc accatgcacc accgcaccgc ttagcgcgat
     2701 tctgctgtat atcgcccaaa gcgggaacat gttgctgggg ggcggcacgc tttatctcta
     2761 tgcgttgggc atgggcctgc cgctgatgct aataaccgtc tttggtaacc gcctgctgcc
     2821 gaaaagcggc ccgtggatgg aacaagtcaa aaccgcgttt ggttttgtga tcctcgcact
     2881 gccggtcttc ctgctggagc gagtgattgg tgatatatgg ggattacgct tgtggtcggc
     2941 gcttggtgtc gcattctttg gctgggcctt tatcaccagc ctacaggcca aacgcggctg
     3001 gatgcgcgtg gtgcaaataa tcctgctggc agcggcattg gttagcgtgc gcccacttca
     3061 ggattgggca tttggtgcga cgcataccgc gcaaactcag acgcatctca actttacaca
     3121 aatcaaaacg gtagatgagt taaatcaggc gctcgttgaa gccaaaggca aaccggtgat
     3181 gttagatctc tatgccgact ggtgcgtcgc ctgtaaagag tttgagaaat acaccttcag
     3241 cgacccgcag gtgcaaaaag cgttagcaga cacggtatta cttcaggcca acgtcactgc
     3301 caacgacgca caagatgtgg cgctgttaaa gcatcttaat gtccttggcc taccgacaat
     3361 tctctttttt gacggacaag gccaggagca tccacaagca cgcgtcacgg gctttatgga
     3421 tgctgaaacc ttcagcgcac atttgcgcga tcgccaaccg tgaacgacac ttgcagtggg
     3481 aaagacggag gaaaatagcg tgcaacgtga agatgtactg ggagaagccc tgaaattatt
     3541 agaattacaa gggattgcca acaccacgct ggagatggtt gctgaacgtg tggattatcc
     3601 actggacgag ctacgccgct tctggccaga caaagaggca atcctctacg atgcgctgcg
     3661 ttatcttagc caacagatag atgtctggcg tcgtcagctg atgctggacg aaacgcaaac
     3721 cgccgaacaa aagttgctgg cacgttatca ggcgttatcg gagtgcgtta aaaacaaccg
     3781 ctatccgggc tgtctgttta tcgctgcctg tacgttttat cccgatcctg gccaccctat
     3841 tcatcaactg gccgatcagc aaaaaagcgc ggcctacgat ttcacccacg aactattaac
     3901 cacactggaa gttgacgatc cggcgatggt tgcaaagcag atggaactgg tgctggaagg
     3961 ctgtttaagc cgaatgctgg tgaatcgtag ccaggcggat gtcgacaccg cacatcggct
     4021 ggcggaagat atcctgcgct tcgcccgctg ccgccagggt ggtgctttga cctgacagaa
     4081 acacagaaaa gaagcgattt gccgcaatct taagcagttg aatcgctttt actgaaatta
     4141 ggttgacgag atgtgcagat tacggtttaa tgcgccccgt tgcccggata gctcagtcgg
     4201 tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat tccgagtccg ggcaccaagt
     4261 tcatatcaac ggacctccac ggaggtccgt ttttcgtttc agaacgccac aatttaagcg
     4321 ttctgcctcc aaatcaattc taccgaactc aaccagattc cccccacatc aaccccattg
     4381 tgtgggtata attgcgggta taccccagtt cgacagaatt tgtaccctct ttaacgcatt
     4441 cagaaggctg aactatggca ctgactgacg caaaaatccg ggctgcaaag cccactgaca
     4501 aggcttataa actcactgac ggggctggca tgttcctgct ggtacatcct aatggttccc
     4561 gttactggcg tctccgttat cgtattctgg gtaaggagaa aaccctggca cttggtgtgt
     4621 atccggaagt ttctctctcc gaagctcgta caaaacggga tgaggcccga aaactgattt
     4681 cggagggggt tgacccttgc gaacagaaaa gagctaaaaa agtagtccct gatttacagc
     4741 tctcttttga acatattgca cgacgctggc atgccagtaa taaacaatgg gcacaatcac
     4801 acagcgataa agtactcaaa agcctcgaga ctcacgtttt cccctttatc ggcaaccggg
     4861 atatcacaac actcagtacc ccggatctgc ttatccctgt tcgtgctgca gaagttaaac
     4921 aaatttatga aatcgccagt cgtctgcagc aaagaatatc tgctgtaatg cgttatgccg
     4981 tacagtctgg catcatcaga tataacccgg ctctggatat ggctggtgca ttgaccacgg
     5041 taaaacgcca gcatcgccct gctctggatc tttcacgcct gcctgaactt ctgtcgcgta
     5101 ttgacggtta taaaggccag cctgtcaccc gactggcagt tatgctgaat ttactggttt
     5161 ttattcgttc cagtgaactc agatatgccc gctggtctga aattgatatt gacaatgcca
     5221 tgtggactat tccagccgaa cgcgaacctc tgccaggcgt aaaattttca taccggggct
     5281 ccaagatgcg aacaccacat cttgtgccac tcagccaaca ggctgtagcc atactggcag
     5341 aacttcagac atgggcaggt gaaaatggtc tgatctttac gggagcacac gacccacgta
     5401 aaccaatcag tgaaaatacc gtaaataagg
//
LOCUS       DAOTRK010000224         5405 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.226, whole genome shotgun sequence.
ACCESSION   DAOTRK010000224 DAOTRK010000000
VERSION     DAOTRK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.226"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(69..350)
                     /locus_tag="QIE41_004460"
     CDS             complement(69..350)
                     /locus_tag="QIE41_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDS3533247.1"
                     /translation="MNKYWLSGIIFLAYGMASPAFSSQTATLTINGRISPPTCSMAMV
                     NGQPQQHCGPLTYDVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(613..1722)
                     /gene="apbC"
                     /locus_tag="QIE41_004461"
     CDS             complement(613..1722)
                     /gene="apbC"
                     /locus_tag="QIE41_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDS3533248.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1854..3887
                     /gene="metG"
                     /locus_tag="QIE41_004462"
     CDS             1854..3887
                     /gene="metG"
                     /locus_tag="QIE41_004462"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDS3533249.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELTDPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4028..>5405
                     /locus_tag="QIE41_004463"
     CDS             4028..>5405
                     /locus_tag="QIE41_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HDS3533250.1"
                     /translation="MKHFIYQDEKSHKFWAVEQQGNELHICWGKVGTKGQNQVKSFAD
                     AAAAAKAELKLIAEKTKKGYVENALANVHIPPITKATPEVETSPESKNKRPWVADDAV
                     IPVTGDINRFAFPHRSRPREINYLYKDGQIWKRIANNTRAYDPDNNYRSYPENWQLAF
                     AELQRRILNNQQTGSIQSDAALLWNLWNSYSTDELVDDLVIRCGLETTVEIALLALQL
                     KYKPVKADVTTIIPPDLKAESLPNWHQRLCHHLSLASEDEWQRCVDKVLAAIPSLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDDAALTTAEKYDFPPLYHD
                     FRAYLAMLLANNGVRGVSRILLEFTEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAALAAYATLLAIHEDAQWRKALVKLITATPDL
                     VTDVIPWVN"
BASE COUNT         1409 a         1362 c         1307 g         1327 t
ORIGIN      
        1 aaaagctgaa atcattcaca cggatgattt cagctttcta aattcgatta aaaaaccagg
       61 gtggcaaatt aatcgtagcg atttaacacg attcgacgtt tgctatcgct acctgcaaca
      121 accacttctg tcgttacacc tttgactggc gaagaaacct gatgacgggt atcaacatca
      181 tacgttaacg gaccacaatg ctgttgaggt tgaccattaa ccatagccat actgcatgtt
      241 gggggactga tcctgccatt aatcgttaat gtggcagtct gtgaagagaa cgcgggagaa
      301 gccattccat aggcaagaaa aataattcct gacagccagt acttattcat tgaacgttat
      361 ccctgtagta aaggttatgc ctggatagaa tgagtgcata acaaactata gctgtacatc
      421 cactacacag ccacgaagga tgataatgaa gcattgcctg tacgatcaat cgactttata
      481 gagtttcgga cgaaggtccg cagaatattc gcagtattaa ataagtgttc attaatgcgg
      541 caaaaacaaa tacaccgcat caatgtaaca tctctgcggt gtatgaagaa taaccagaat
      601 ggttaatagg cgttagaccg cgcggaagga aatttcgcct ggaatgactt caccctgcca
      661 gtagagctga gctgcaacgc ggtcagccag ctggcggtag atagcggtaa attcgctctc
      721 cggacggctg ataaccgttg gcgtgccttt atcgagatcc tcacgtaagg agatgtgtaa
      781 cggcatctgc cccagcagct gtgtgtggta tttttcagcc agtttctcag caccaccggt
      841 accgaaaatc ggttcatggt gaccacagtt actgcaaata tgcacactca tgttctcgac
      901 aataccgagc accggaacct cgactttctc gaacatcaca atgccttttt tcgcatcgat
      961 caacgcaatg tcctgcgggg tggtgaccac taccgcgcca gttactggaa tgttttgtgc
     1021 cagtgtcaat tgaatgtcac cggtgcctgg cggcatatca agcaccagat agtcgagatc
     1081 cggccacaag gtttcctgca acatctgcat taacgccttg ctcgccatcg gaccacgcca
     1141 caccatcgca ttgtcgtcag tgaccagata accaattgaa ttggttgcca gtccatgaga
     1201 cataatcggt gccatatggg taccgtccgg cgaagttgga cgctgatttt ctgcacccag
     1261 catggtgggt attgatgggc cgtagatatc ggcatccaga atgccgactt tcgccccttc
     1321 cgccgccagt gccagcgcca ggtttaccgc cgtggaggat ttacccacgc cgcctttacc
     1381 tgagctgacc gcaatgatgt tcttcacgcc gttaattccc ggctgattct tcacgcgttt
     1441 taaggtggcg atattgtgcg acagtttcca gtcgatagct ttcgccccgg tgatgcgcag
     1501 cagttcggcg ctacattgct cttttaactc ttcgaacgcg ctatgccaga cgaatggcat
     1561 aaccagttcc acatgcaacg tgtcgtccat ccaggcgaca tggtgcaacg ctttcagcgt
     1621 ggtgaggtta tgcttcaggg ttgggtgctg aaaattggcc agtgtcccgg cgaccattgc
     1681 gcgcaaggct tctggtgatt tggcctggga ttgttcgttc atcccgactc cttttttgta
     1741 tagataaacc atcagctgat agtttacctg aagaatatag agaagtactt acttaacatt
     1801 ttcccatttg gtactatcta accccttttc actattaaga agtaatgcct actatgactc
     1861 aagtcgcgaa gaaaattctg gtgacgtgcg cactgccgta cgctaacggc tcaatccacc
     1921 tcggccatat gctggagcac atccaggctg atgtctgggt ccgttaccag cgaatgcgcg
     1981 gccacgaggt caacttcatc tgcgccgacg atgcccacgg cacaccgatc atgctgaaag
     2041 ctcagcagct tggtatcact ccggagcaga tgattggcga aatgagtcag gagcatcaga
     2101 ctgatttcgc aggctttaac atcagctacg acaactatca ctcgacgcac agcgaagaga
     2161 accgccagtt gtcagaactt atttattctc gcctgaaaga gaacggtttt attaaaaacc
     2221 gcaccatctc tcagctttac gatccggaaa aaggcatgtt cctgccggac cgttttgtaa
     2281 aaggcacctg cccgaaatgt aaatccccgg atcaatacgg cgataactgc gaagtttgtg
     2341 gcgcgaccta cagcccgact gagctgatcg agcctaaatc ggtggtttct ggtgctacgc
     2401 cggtaatgcg tgattctgaa cacttcttct ttgatctgcc ctctttcagc gaaatgctgc
     2461 aggcatggac ccgcagcggc gcgttgcagg agcaagtggc aaacaaaatg caggagtggt
     2521 ttgaatctgg cctgcaacag tgggatatct cccgcgacgc cccgtacttc ggttttgaaa
     2581 ttccgaatgc accgggcaaa tatttctacg tctggctgga tgcgccgatt ggctacatgg
     2641 gttccttcaa gaatctgtgc gacaaacgcg gcgacaccac cagcttcgat gaatactgga
     2701 agaaagattc caccgccgag ctgtatcact tcatcggcaa agatattgtt tacttccaca
     2761 gcctgttctg gccagcaatg ctggaaggca gcaacttccg caagccgact aacctgtttg
     2821 ttcatggcta tgtgacggtg aacggcgcga agatgtccaa gtctcgcggc acctttatta
     2881 aagccagcac ctggctgaat cattttgatg ctgacagcct gcgttactac tacactgcga
     2941 aactctcttc gcgcattgat gatatcgatc tcaacctgga agatttcgtt cagcgtgtga
     3001 atgccgatat cgtgaataaa gtggttaacc tggcatcccg taacgctggc tttatcaaca
     3061 agcgttttga cggcgtgctg gcaagcgaac tgactgaccc gcagttgtac aaaaccttca
     3121 cggatgccgc tgaagtgatt ggtgaagcgt gggaaagccg cgaatttggt aaagcgatcc
     3181 gcgaaatcat ggcgctggca gatctggcta accgttatgt cgatgaacag gctccgtggg
     3241 tggtggcgaa acaggaagga cgcgatgccg acctgcaggc aatttgctcg atgggcataa
     3301 acctgttccg cgtgctgatg acttacctga agccggtact gccgaaactg accgagcgcg
     3361 cggaagcatt ccttaatacg gaactgacct gggatggtat ccagcaaccg ctgttgggcc
     3421 ataaagtgaa tccgttcaaa gcgctgtata accgcatcga tatgaagcag gttgaagcac
     3481 tggtggaagc gtctaaagaa gaagtgaaag cagctgccgc gccagttact ggcccactgg
     3541 cagatgatcc gattcaggaa accatcacct ttgacgactt cgctaaagtt gacctgcgcg
     3601 tggcgctgat tgaaaacgca gagtttgttg aaggttctga caaactgctg cgcctgacgc
     3661 tggatctcgg cggtgaaaaa cgcaatgtct tctccggtat tcgttctgct tacccggatc
     3721 cgcaggcact gattggtcgt cacaccatta tggtggctaa cctggcacca cgtaaaatgc
     3781 gcttcggtat ctctgaaggc atggtgatgg ctgccggtcc tggcgggaaa gatattttcc
     3841 tgctaagccc ggatgccggt gctaaaccgg gtcatcaggt gaaataatcc cccttcaagg
     3901 cgctgcatcg acagcgcctt ttctttataa attcctaaag ttgttttctt gcgattttgt
     3961 ctctctctaa cccgcataaa tactggtagc atcagcattc aactggataa aattacaggg
     4021 atgcagaatg aaacacttta tctatcagga cgaaaaatca cataaattct gggcggtgga
     4081 gcagcaggga aacgagttgc atatctgttg gggaaaagtt ggcaccaaag ggcaaaacca
     4141 ggtaaaaagt tttgcggatg ctgcggcagc ggcaaaagcg gagcttaagc tgatcgcaga
     4201 gaaaacgaaa aaaggctatg tggaaaatgc tttagcaaac gtgcatatcc cccccatcac
     4261 caaagccact cctgaagttg aaacctcccc tgagagtaaa aacaaacgcc catgggtggc
     4321 tgatgatgca gtcattccgg taactggcga tattaatcga tttgcttttc ctcatcgctc
     4381 tcgccccaga gagatcaatt atctgtacaa agatggccaa atatggaagc gcatcgcgaa
     4441 taacaccagg gcatatgacc ccgacaataa ttaccgctct tatcctgaaa actggcagct
     4501 ggcgtttgcc gagttacaaa ggcgaattct gaataatcaa caaacaggaa gtatacaatc
     4561 tgatgcggca ttgctctgga atttatggaa ctcttactct acagatgaac tggtcgatga
     4621 cttagtcatt cgctgtggtc tggaaactac cgttgaaatc gcccttctcg ctcttcaact
     4681 caaatataaa ccagtaaaag ccgacgtcac caccattatt ccccctgatc tcaaagcaga
     4741 atcactaccg aactggcatc aacgtctatg tcatcatctt tcactcgctt cagaagatga
     4801 gtggcaacgc tgtgtagata aagttctcgc tgctattccc tcgctatccc cagcacggca
     4861 accttttgcg gcgttactgc tccctgaacg cccggatatt gccaatgcta tggcgctacg
     4921 ttatgcagat caaaacgttc cggcgatgac ctggttaaga ctggtggtaa ccgatgacgc
     4981 cgctctgact acagcagaaa aatacgactt cccaccgttg tatcatgatt ttcgcgctta
     5041 tctggcaatg ttgctggcaa ataatggggt gcgcggtgta agccggatac ttcttgagtt
     5101 tacggaagag cattctgaca atcccactta tctcttcgag cgcatcagcg agactgaaga
     5161 tcttgtcaaa tggctatgga aaacgaatca cccggatgcg attcaaattc tgatcctcgg
     5221 tgtaattggc aagaaaaagc acctggaata cttaagcaaa gcctgtcaaa aacatcccgc
     5281 tgcggctctt gccgcttatg caactttact ggcaatacat gaagatgcac agtggcgtaa
     5341 agcgcttgtc aaactgatta ccgccacgcc agatttagtc actgacgtca ttccctgggt
     5401 taatg
//
LOCUS       DAOTRK010000225         5362 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.227, whole genome shotgun sequence.
ACCESSION   DAOTRK010000225 DAOTRK010000000
VERSION     DAOTRK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.227"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(599..1084)
                     /locus_tag="QIE41_004464"
     CDS             complement(599..1084)
                     /locus_tag="QIE41_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3533251.1"
                     /translation="MIRIAFLITNKFLDIDSTTLAGYDFSGMDLHRAILNGYCLDGAK
                     FENSHLRNVMIQHTSTRNAVFRNAALMNAILNNSDFTGSDFSNSRSIGANFEGAILTN
                     STFCNADVKDSNFSGADLCGANLLCDRYGDINITGALFDKNTSWLEGFDPIAHGAILK
                     I"
     gene            complement(1081..5292)
                     /locus_tag="QIE41_004465"
     CDS             complement(1081..5292)
                     /locus_tag="QIE41_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDS3533252.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPV
                     EEQKGNPVNPLLGAKVLPGETDLAQPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQVHERELILNDSGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSA
                     LWQVLPESLRLSPHVYLATNSLQGPWWILGWPERVPEADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGEAAGAVTGVTDGAGRRFHLALTTQAQRAKAFRKQRESSLSSPASP
                     RSVSSSQFFPDTLPTGTEYGADNGIRLEAVWLTHDPAYPDELPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEYGDGSITRSEYDEAGRLKAQTDAA
                     GRRTEYRLHMASGKLTSVILPDGRTVRYGYNNQLQLTSVTYPDGLRRSRKYDRQGRLA
                     EEVSRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTDCSGYTTRYEY
                     DRYGQRTAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIIAPNGSR
                     SETQYDAWGKAVSTTQGGLTRSMEYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYRYSATGQLIRSEDEGQVTQWYYDDAGRITHRTVNGDPAEQWQYDDHGWLTE
                     ISHLSEGHRVAVHYGYDDKGRLTGERQTVENQETGEMLWEHETRHVYSEQGLATRQEP
                     DGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYT
                     LTGQLRSRHLNLPQLDREYTWNDNGQLVRISGPQESRKYRYSDTGRLTGVHTTAANLD
                     IDIPYATDPAGNRLPDPELHPDSTFTAWPDNRITEDAHYVYRYDEYGRLAEKTDRIPE
                     GVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDL
                     TGWMSLSRKPEETWYGWDGDRLTTVQTGTTRIQTVYQPGSFTPLIRIETENGEQAKAR
                     HRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLQQCGLTAEQMKNQM
                     EAEYIPERTLHLYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQQLQQPYRL
                     PGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGAWNLYQYPLNPTTKIDPLGLSG
                     EDSIEQSGPSWLIADPMDKYRQQAVMGLTKLWDDTFSQEKTKCLPTTPENIRMVVANS
                     PLLTVQQKVSVPVVAHYVQMLEDGSGAPPVDMDGNVIVNGNHRMVAGLLCDKLPPERP
                     GTRPLSAPLYPFSQIFPELDDWGNR"
BASE COUNT         1033 a         1722 c         1326 g         1281 t
ORIGIN      
        1 ttttaagttc cattttgttc tccttaatta tgtaaggagt atttgatcat gtatacgcaa
       61 taaaaaacag cttcaggtaa tgaggaatat ctcaattgtt aaacataaaa tgcgaattgt
      121 ctagtacaaa aaagcagcgt tacgtgatct ttccgtggat cgtcaaattg ttcgagagaa
      181 aataccaaca tgatggattt tactgtaaaa gtaatgtcgt agtttaaaaa ctcatcatcc
      241 ggtaaagcct tgtggggcgt gggctttcat acttttaccc tgatgtctgc tatacgccgg
      301 acacccttta cgtagcattc ggaattcacc cgtaaattta tcacggcggt atcacgaaac
      361 ctccggttct gatgccgctt aggactaacg acacgtcaac acaggttcgg gtttattcat
      421 ttcactgtat ctcatataat ctgagcactc cccctgcgct cgctactatg actctttacc
      481 acagcaaccg gggttgtttg ggatcggctt tcaacagcag atcccggtgg ctattgcaag
      541 taagtctact ttccacatca gtgttactga cacagaaaat aatgagttat gatccttgtc
      601 atatttttaa tatagcccca tgagctatag gatcaaaacc ttctaaccat gaggtgtttt
      661 tatcgaataa tgcacctgtt atatttatat cgccatatct atcacataat aaattagcgc
      721 cacataagtc agcaccagaa aaatttgaat ctttaacgtc tgcattgcaa aaggttgaat
      781 tagttaatat agcaccctca aagtttgcac ctattgacct tgaattacta aagtcacttc
      841 cagtgaaatc tgagttatta agaatagcgt tcatcagagc cgcattgcga aagacagcat
      901 ttctagttga tgtatgttga atcattacat ttcttaaatg ggaattttca aatttcgccc
      961 catcaagaca ataaccattg aggatggcac gatgtagatc catcccgcta aaatcatatc
     1021 cagcaagagt ggtactatct atatcaagaa atttattggt tattaaaaac gcgattctta
     1081 tcatctattc ccccaatcat ctaactccgg aaaaatttgg ctaaacggat atagtggtgc
     1141 cgataatgga cgcgtacctg gccgctcagg aggaagttta tcacataaca gacccgcaac
     1201 catcctgtga ttaccattaa caatcacatt cccatccata tcaaccggtg gtgctccact
     1261 accatcttcc agcatttgta cgtagtgagc tactacgggt actgagactt tttgttgtac
     1321 tgtcaacaag ggactgttgg ccacaaccat cctgatgttt tcaggtgtcg taggtaaaca
     1381 tttagtcttc tcttgactaa aggtatcatc ccacaatttt gttaaaccca tcactgcctg
     1441 ttgtctgtat ttatccatgg ggtcagcaat caaccaagat ggacctgact gctctatgga
     1501 atcttctccg gagagaccca acggatctat cttagtagtt ggattcaatg gatactgata
     1561 caggttccat gcacctctta gcccaatggg atcctgggtg atataccgcc cctgcaacgg
     1621 gtcatagtac cggttgcggt tatagtacag ccccgactcc tcatcatact gctgccccgg
     1681 cagacggtac ggctgttgca gttgctgcgc gctggtttcg cccagcaggt ttccccactc
     1741 gtcatactcc ccctgccacg ccgtttcccc ttccgggctg atgagcgcct gcggcagccc
     1801 ccggtggtcg cagtggtaaa gatgaagtgt cctctccggg atgtattccg cctccatctg
     1861 gtttttcatc tgttccgccg tcagcccgca ctgctgaagc catgcctcac tctccgcact
     1921 caccgcaccc gcgcgcagtt cccgctccag cctccccagc atcaccgaca gctccgccgg
     1981 tagcgtcacc cccgtgtcct cctgcaacac ctccgccaga ctccggtgcc gcgccttcgc
     2041 ctgttcacca ttctctgttt cgattctgat gagcggcgtg aagctccccg gctggtatac
     2101 cgtctggata cgcgtggtgc cggtctgtac cgtcgtcagc ctgtcaccgt cccacccgta
     2161 ccaggtctcc tccggtttac gcgacagcga catccacccc gtcaggtcac gctcccgccg
     2221 ccacacccgt ttccccgtcc ggcggcccag cgggtcatag aggtagcggc tctccacctg
     2281 tggctcgccg tgctgtatcc gcgtgtagaa caccaggcgg tgctggctgt cgtaatggta
     2341 gtggtgcgtc cgctcgtcgt gcatccgtat caccccttcc gggatacggt ccgtcttctc
     2401 cgccagcctg ccgtattcat cgtagcggta gacatagtgc gcatcttccg tgatgcggtt
     2461 atccggccac gccgtgaagg tgctgtccgg gtgcagttcc gggtccggca gccggtttcc
     2521 tgccgggtcc gtcgcatacg ggatatcgat atccaggttc gccgccgtgg tgtgaacgcc
     2581 cgtcagtctt cccgtgtcgc tgtaacggta cttccggctc tcctgcgggc cgctgatgcg
     2641 caccagttgc ccgttatcgt tccacgtata ctcacggtca agctgcggaa ggttcaggtg
     2701 cctgctccgg agctgccctg tgagggtgta cgccgtggcc tgttcgtacc ccgctgtact
     2761 gcccgccccg ccgaagctgc gggccgtctc acggtgcagc cggtcccgcg tgtactcgac
     2821 cagcggcgtt ccgcccagct tcatccccgc aagataaccg ctgccatacg tcagccactc
     2881 caccggcggc agaccgtccg gctcctgacg ggtcgccagc ccctgttcgc tgtagacgtg
     2941 cctcgtctca tgctcccaca gcatctcccc cgtctcctgg ttctccaccg tctgccgttc
     3001 gcccgtcagg cggcctttat cgtcatagcc gtagtggacc gccacacggt ggccttcgct
     3061 caggtggctg atttctgtca gccagccgtg gtcgtcatac tgccactgct ccgccgggtc
     3121 gccgttcacc gtccggtgcg tgatgcgccc ggcgtcatcg tagtaccact gcgtgacctg
     3181 cccctcatcc tcgctgcgga tgagctgccc tgtggcgctg taccggtaac gctgtgtcct
     3241 gccgtcaaag ccgcgctgct ccgtcagcct gtccaccggg tcataccgga acgtggactg
     3301 gctgccgttc tcgttggtca gcacggtgat gcgcccggcg gcgtcatact ccatgctgcg
     3361 cgtcagtccg ccctgcgtgg tgctgacggc ctttccccac gcatcatact gtgtctcact
     3421 ccggctgccg ttcggggcaa tgatagcggt gaggtcgcct gcggcgctgt actcataacg
     3481 ggtttcacgt ccctgcgcat ccttctgact gaccagttga ccacgcgggt tataactgct
     3541 gtaagtgctg atgccttcct cccggtgaac cgccgtcctc tgaccgtacc ggtcgtattc
     3601 ataccgcgtc gtgtaccctg aacagtccgt aaatgcctga agctgaccgt accggtttcg
     3661 cgttattctc cgcctcaccc cggttccgtc ttccaccgca cagggcaggc cactgcggga
     3721 agaatcataa aaccagcgcg taatgttacc gttgcgcgag acctcttccg ccagcctgcc
     3781 ctgacggtca tatttccggc tgcggcgcag cccgtccggg tacgtcactg acgtcagttg
     3841 caactggttg ttgtaaccat accgtaccgt cctgccgtcc ggcagtatca ctgaagtcag
     3901 ctttcccgac gccatatgca gccggtactc cgtccgccgt cccgccgcat ccgtctgcgc
     3961 cttcagcctc cccgcctcat catactcgct gcgggtgatg ctcccgtccc catactcctt
     4021 cttcaccacc cgcttcaggc cgccctcgcc ctccgtgtac agcacctccc gccggttcag
     4081 gctgtccgtg atggtcacgc ggtcctgccc gtactcaaag cggtagtcca gcccctccgg
     4141 gttgacctgc tccgtcaccc gaccggtatc atcataccgg tagcggctct ccggcctccc
     4201 cgcataatgg tgcgccacca tccgcccggc gtgctccgca tcataagtaa acccgcgcac
     4261 ctgcgtcccg ctgcggtcat acaccgcccg cagttctccg ctggccgtgt acgtgtagcg
     4321 cgccagcggc gcagtcggca gttcatccgg gtatgccggg tcgtgtgtca gccataccgc
     4381 ctccagccgg ataccgttat cggcaccgta ctctgtaccg gtgggcagcg tgtcggggaa
     4441 aaactgagaa gaggaaacag aacggggact ggccggagaa gataaagaag actcgcgctg
     4501 tttacggaag gctttcgccc gctgcgcctg tgtggtcagc gccagatgaa aacggcggcc
     4561 cgcgccgtcc gtcacccccg tcaccgcgcc cgccgcttcc ccctctgccg cccggtggaa
     4621 ggccagcgtc cgcccgaagc cgtccaccac gcccgtcagc acccggtatg cgggcggctc
     4681 cggcggcagc acctcgtccg cctccggcac ccgctccggc cagccgagta tccaccacgg
     4741 cccctgaagg ctgtttgtcg ccaggtacac atgcgggctt aagcggagac tctccggcag
     4801 cacctgccag agcgcgctta gcggctgcga actgtgctgc tccgccaccc cgccccttgc
     4861 cagccagaac gactcgctgc ggctgtagct tatctcgccg ggaaacagcg gctcaaagtg
     4921 aatgctcctg ccgccactgt cgttgagtat cagttcccgc tcatgaacct gtaagcggat
     4981 atcgaagggc gctttccagc cgggaccaaa cacccccacc ggcgcgggtg tcctggtccg
     5041 gtagctgctg taggcgcggg aaagaataaa cggcaacggg ccgggctgcg caagatccgt
     5101 ctcgcccggc agcaccttcg cacccagtag cgggttcacc gggttaccct tctgctcctc
     5161 caccgggctg ttgggacaca ccgagcaggc cactcccgtc ggcgcaccta tcagcacccc
     5221 cgctgaaccc tgcacaatat ccagtccctt gcgggtcata tcgccctgtc gcgccgccgg
     5281 ttttccgctc atggtttatt ccttaaatta tttatggttt tattctgagt gcaatccata
     5341 ctgattcata accagtgaat aa
//
LOCUS       DAOTRK010000226         5304 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.228, whole genome shotgun sequence.
ACCESSION   DAOTRK010000226 DAOTRK010000000
VERSION     DAOTRK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.228"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..95)
                     /locus_tag="QIE41_004466"
     ncRNA           complement(<1..95)
                     /ncRNA_class="other"
                     /locus_tag="QIE41_004466"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(142..273)
                     /locus_tag="QIE41_004467"
     ncRNA           complement(142..273)
                     /ncRNA_class="other"
                     /locus_tag="QIE41_004467"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(320..404)
                     /locus_tag="QIE41_004468"
     tRNA            complement(320..404)
                     /locus_tag="QIE41_004468"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Tyr,seq:gta)
     gene            complement(439..523)
                     /locus_tag="QIE41_004469"
     tRNA            complement(439..523)
                     /locus_tag="QIE41_004469"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(487..489),aa:Tyr,seq:gta)
     gene            complement(682..1524)
                     /gene="purU"
                     /locus_tag="QIE41_004470"
     CDS             complement(682..1524)
                     /gene="purU"
                     /locus_tag="QIE41_004470"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDS3533253.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1574..2032)
                     /locus_tag="QIE41_004471"
     CDS             complement(1574..2032)
                     /locus_tag="QIE41_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDS3533254.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARYTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            2145..3050
                     /gene="rssA"
                     /locus_tag="QIE41_004472"
     CDS             2145..3050
                     /gene="rssA"
                     /locus_tag="QIE41_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDS3533255.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3142..4155
                     /gene="rssB"
                     /locus_tag="QIE41_004473"
     CDS             3142..4155
                     /gene="rssB"
                     /locus_tag="QIE41_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDS3533256.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            4357..5265
                     /gene="galU"
                     /locus_tag="QIE41_004474"
     CDS             4357..5265
                     /gene="galU"
                     /locus_tag="QIE41_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDS3533257.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
BASE COUNT         1339 a         1201 c         1373 g         1391 t
ORIGIN      
        1 cgcccttcgg gtcgttgcct gcggcaacgc tctctcgctg gcgctcaagt cgaaccttgg
       61 ttgaagcttc tcatccttcc ccgcatgggc agaatatttg attgcggatt cgtttgagaa
      121 ttccggggct tttgaaagtt atggtggtgg gggaaggatt actcagcgct gcgcgcttcg
      181 cccttcgggt cgttgcctgc ggccacgctc tctcgctggc gctcgagtcg aaccttggtc
      241 gaagcttctc atccttcccc gcatgggcag aatatttaat cgcggattcg tttgagagtt
      301 cacggacttt tgaaagtgat ggtggtgggg gaaggattcg aaccttcgaa gtcgatgacg
      361 gcagatttac agtctgctcc ctttggccgc tcgggaaccc caccaggggt aattcaaatt
      421 ttgaggtaat gcttgagatg gtggtggggg aaggattcga accttcgaag tcgatgacgg
      481 cagatttaca gtctgctccc tttggccgct cgggaacccc accacggggt aatgctttta
      541 ctggcctgct cccttatcgg gaagcggggc gcatcatatc aaatgacgcg ccgctgtaaa
      601 gtgttacgtt gagaaaaatg aactggttgg gtaattttca tccgtaacgg attaaaggta
      661 accagttatt tttgctggcg attaaagaat aatcgtccga ttaccgtaaa caaagacgcg
      721 ctgcgccagt actttgtaga gtgcgcgact taagacgttt ttctcgacgt cacgacctgc
      781 gcgcatcata tcttcagctg tgtaggtatg atcgacatga ataacgtcct gcatgatgat
      841 tggaccttcg tccagattgt cattcacata gtgagcggtt gcgccaataa tcttcacgcc
      901 acgttcatag gcctggtgat aaggacgtgc gccgataaac gctggcagga atgaatggtg
      961 aatattgatg atcttattcg ggaagcgtgc cacaaattct ggcgttaata cccgcatata
     1021 cttcgccagc accacgtagt caggctgata agcatcaatg gcatccgcca tcttttgatc
     1081 gtgctcgttg cggcttaacc cttcatggct taccagctca aacggaatat caaaacgctc
     1141 aaccagagaa cgtaaagtat cgtggttacc aataactgcc gcgatttcga catccaggcc
     1201 gccgtaattg gctttcatca acaaatcgcc gaggcaatgc gcctctttag tgaccagaat
     1261 cactatccgg cgacgaccgg caggattcag ctcacgtacg gagccttctg gcaatgcgct
     1321 gtcgagatcc gccagcaggg tggaatcatt aaaaattcct tccagttccg tgcgcataaa
     1381 aaagcgcccg gtacggtgat caacaaattc attgttctgt acgatattta actcgtgctt
     1441 gtagcaaata ttggtaatac gtgcgatcag acctttttgg tccggacaaa tagtacgcag
     1501 aactttacgt tggagtgaat gcattgctgg aaaaaccttg ttgagagtgt ttgctaaacc
     1561 gtaccgtcaa ccattattgg ccgcagcact ttttaaattt tttacctgaa ccacaagggc
     1621 agggatcgtt gcggccaaac tgcggacgtg taccgtcaat atagtaccat tgaccgtttt
     1681 ctttcaaaaa tcgcgaacgt tcaatgattg cacctgtttt gccaccttcg gtatatcgcg
     1741 caacaaaact gacaaatccg atattgtcga cgtcctgcca acaatgttca aatacggtca
     1801 atcctaacca ttcagtatgc gcaaatccgg caatcaattc ggcacgtaac gccgccgctc
     1861 cacaagatgg atgccaggtc tttattaaat aatctgcgtc tttcatcaca aaagcgcagt
     1921 aacgcgaacg catgagatgt tctggatcag gtgcaacctt ttcaccagac acataagggt
     1981 ggcaacatag gctatactcg acagcactac cacagggaca aagctgagac acaaataatc
     2041 tccctggaaa caataacggc gtattaaccg cctgagtagc actatgttaa ccgagcagta
     2101 gcgatgttgc tacgattgca ttccagggga atcttgcggg aataatgaga aagataaaaa
     2161 tagggctggc gctgggatct ggcgcggcga gaggttggtc gcatattggc gttattaatg
     2221 cgctaaaaaa agtgggtatt gaaattgata tcgttgcagg atgttcaatt ggttcgctgg
     2281 tgggcgctgc ctatgcatgc gatcgactat ctgcgctgga agattgggtg acctctttca
     2341 gttattggga tgttttacgc ctgatggatc tctcctggca gcgcggtggg ttactgcgcg
     2401 gcgagcgtgt cttcaatcaa tatcgcgaaa taatgccgga aacagagatc gaaaattgtt
     2461 cccgtcgctt cgcggctgtt gccaccaatt taagtactgg gcgcgaatta tggtttactg
     2521 aaggcgatct ccatcttgct attcgtgcat catgcagtat tccaggacta atggcacccg
     2581 ttgcgcataa cggctactgg ctggttgatg gcgctgtagt taacccaatt cctatttcgc
     2641 tcacgcgtgc attgggtgct gatattgtga tagcggtcga cctgcagcac gatgctcatt
     2701 tgatgcaaca agatctgctc tcctttaatg ttagtgaaga aaatagcgag aatggtgatt
     2761 ctctgccgtg gcatgcgcgt ctgaaagaaa ggttgggcag cataacgaca cgtcgggcgg
     2821 tgacagcgcc aacggcaaca gagattatga ccacttctat ccaagtgctg gagaaccgcc
     2881 ttaaaaggaa ccgcatggca ggtgatccgc ccgatattct gattcaacct gtttgcccgc
     2941 aaatatctac gcttgatttc catcgcgcgc acgctgccat tgcggctggg cagctggcag
     3001 tggaaaagaa aatggatgaa cttttgccgt tggtacgcac caacatttga ccagaatttt
     3061 tatctacact taagttaatt ctgacaggcg caggtggcaa tagcatgcca ctattgagta
     3121 aagccagtca ggggagagaa catgacgcag ccattggtcg gaaaacagat tctcattgtt
     3181 gaagatgagc aggtatttcg ctcgcttctg gattcgtggt tttcctcatt gggagcgaca
     3241 acggtactgg cggctgatgg ggtggatgcc cttgagttgc tgggaggttt cactccagac
     3301 ctgatgatat gtgatatcgc gatgccacga atgaacgggc ttaaactgct ggagcatata
     3361 cgtaacagag gcgaccagac acctgttctg gtgatatctg ccactgaaaa tatggcagat
     3421 attgccaaag cgttacgtct gggcgttgaa gatgttttgc tgaaaccagt taaagatctg
     3481 aatcgcttgc gcgagatggt ttttgcctgt ctctatccca gcatgtttaa ttcccgcgtt
     3541 gaggaagagg agaggctttt tcgcgactgg gatgcaatgg ttgataaccc tgccgcagcg
     3601 gcgaaattat tacaggaatt gcaaccgccg gttcagcagg tgatttccca ttgccgggtt
     3661 aattatcgtc aattggttgc cgcggacaaa cccggcctgg tgcttgatat tgccgcactt
     3721 tcggaaaacg atctggcatt ttattgcctt gatgtcaccc gagctggaca taatggcgta
     3781 cttgctgcct tgttattacg cgcattgttt aacggattat tacaggaaca gcttgcacat
     3841 caaaatcaac ggttgccaga gttgggcgcg ttattgaagc aggtaaacca tttactccgt
     3901 caggccaatc taccggggca gtttccgcta ttagttggct attatcatcg cgaactgaaa
     3961 aatctcattc tggtttctgc gggtctgaat gcgacattaa atactggcga acaccaggtg
     4021 caaatcagta atggtgttcc gttaggcact ttaggtaacg cttatttgaa tcaattgagc
     4081 cagcgatgcg atgcctggca atgccaaata tggggaaccg gtggtcggct tcgcttgatg
     4141 ttgtctgcag aatgagcaaa cgataacgcg ggctaaattt gcattacctg ctaatgtcgg
     4201 ctggtgttac tatcgtcgcc attcgtataa gtaattgtct taattatgct aactcgcctc
     4261 cttttcagaa cttagcccct tcggggtgct gatatactgg gatgcgatac agaaatatga
     4321 acacgttcaa aacacgaaca gtccaggaga atttaaatgg ctgccattaa tacgaaagtc
     4381 aaaaaagccg ttatccccgt tgcgggatta ggaaccagga tgttgccggc gacgaaagcc
     4441 atcccgaaag agatgctgcc acttgtcgat aagccattaa ttcaatacgt cgtgaatgaa
     4501 tgtattgcgg ctggcattac tgaaattgtg ctggttactc actcatctaa aaactcgatt
     4561 gaaaaccact ttgataccag ttttgaactg gaagcaatgc tggaaaaacg tgtaaaacgt
     4621 caactgcttg atgaagtgca gtctatttgt ccgccgcacg tgactattat gcaagttcgt
     4681 caggggctgg cgaaaggcct gggacatgcg gtattgtgtg ctcacccggt agtgggtgat
     4741 gaacctgtgg ctgttatttt gcctgacgtt attctggatg aatatgaatc cgatttgtca
     4801 caggataacc tggcagagat gatccgccgc tttgatgaaa cgggtcatag ccagatcatg
     4861 gttgaaccgg ttgctgatgt taccgcatat ggcgttgtgg attgcaaagg cgttgaatta
     4921 gcgccgggtg aaagcgtacc gatggttggt gtggtagaaa aaccgaaagc ggatgttgcg
     4981 ccgtctaatc tcgctattgt gggtcgttac gtacttagcg cggatatttg gccgttgctg
     5041 gcaaaaaccc ctccgggagc tggtgatgaa attcagctca ccgacgcaat tgatatgctg
     5101 atcgaaaaag aaacggtgga agcctatcat atgaaaggga agagccatga ctgcggtaat
     5161 aaattaggtt acatgcaggc cttcgttgaa tacggtattc gtcataacac ccttggcacg
     5221 gaatttaaag cctggcttga agaagagatg ggcattaaga agtaacatcc gtatcggtgt
     5281 tatccacgaa acggcgttga gcaa
//
LOCUS       DAOTRK010000227         5285 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.229, whole genome shotgun sequence.
ACCESSION   DAOTRK010000227 DAOTRK010000000
VERSION     DAOTRK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.229"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(211..294)
                     /gene="yohP"
                     /locus_tag="QIE41_004475"
     CDS             complement(211..294)
                     /gene="yohP"
                     /locus_tag="QIE41_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDS3533258.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            518..1954
                     /gene="mdtQ"
                     /locus_tag="QIE41_004476"
     CDS             518..1954
                     /gene="mdtQ"
                     /locus_tag="QIE41_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDS3533259.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            2007..2768
                     /locus_tag="QIE41_004477"
     CDS             2007..2768
                     /locus_tag="QIE41_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS3533260.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGVDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGVQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2887..3465)
                     /locus_tag="QIE41_004478"
     CDS             complement(2887..3465)
                     /locus_tag="QIE41_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDS3533261.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3635..4222
                     /locus_tag="QIE41_004479"
     CDS             3635..4222
                     /locus_tag="QIE41_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDS3533262.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     DGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4396..>5285
                     /gene="pbpG"
                     /locus_tag="QIE41_004480"
     CDS             4396..>5285
                     /gene="pbpG"
                     /locus_tag="QIE41_004480"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDS3533263.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKA"
BASE COUNT         1278 a         1332 c         1426 g         1249 t
ORIGIN      
        1 cccggtagcc gtaagggata agccgagggc accgcgcagc ggcgattttg ttcgccagag
       61 cctgggggtg cagggggcgg cggcgattgg tcgccccctg cgcgctcctt gcgccagtga
      121 ttatatgaag caatgcacat gacaggagcg caacaggatg agagactgaa atattctcgt
      181 tattttcgga tctgatgaca ttaattaatt ttaaaatatc atcttaaata cgccagtcac
      241 caccagtagc ccaatcagga aaataatcaa tacagcccag agtataattt tcattggcgt
      301 tcctttattt gtgagtgctg aataagtata ggaaaaaacg aagccctttg ccgctatcca
      361 taaacgatta agataaatcg gaagatatca ttcacttagt gtatattttt ctgaatatgt
      421 tgttttattg ccactgatgt attgccacgc gaaactaaaa aatatatcgc aacacacggt
      481 aatctccgcg caccttttct tacctgatta ttcccccatg aatcgtgatt ctttttatcc
      541 agccatcgcc tgttttccgc tgttactgat gctggccggg tgtgcgccta tgcatgaaac
      601 ccgccaggcg ttaagccagc aaacgcccgc tgtgcaagtt gacaccgcat tacccacggc
      661 gctgaaaaat ggttggccag acagccaatg gtggctggag tatcacgata atcaactcac
      721 ttcattaatt aataacgcac tacagaacgc accggatatg caggtggctg aacaacgcat
      781 tcaactggct gaagcccagg cgaaggcagt tgccactcag gatggtccgc agctcgactt
      841 ttctgcggat atggagcggc aaaaaatgtc ggcagaaggg ttaatggggc cgtttgcttt
      901 gaacgatccg gccgcaggta cgaccggtcc gtggtacacc aacggtactt ttggcttaac
      961 ggcgggctgg catctcgata tctggggaaa gaatcgggcg gaggttactg ctcgcctggg
     1021 tacggttaaa gcacgggcgg cggaacgcga gcaaacccgc caattgctgg ctggcagcgt
     1081 agcccgtctg tactgggagt ggcaaaccca ggcggcgtta aacactgtct tacagcaaat
     1141 cgaaaaagag cagaacacca ttatcgccac cgatcgccag ctatatcaga acgggattac
     1201 ttcttcagtt gaaggtgtgg aaaccgatat taatgccagc aaaacccggc agcagctcaa
     1261 cgatgtcgcg gggaaaatga aaattattga ggcacggtta agcgcactta caaatcacca
     1321 gacaaaggca ttgaagctta atccggtcgc gttgccgaaa gtggcaagcc agcttcctga
     1381 tgaactgggg tactccttac tggcccggcg ggcagatttg caggcggcgc actggtacgt
     1441 tgagtcatcg ttaagcacta ttgatgcggc aaaagcggca ttttatcctg acatcaacct
     1501 gatggccttc ctgcaacagg atgcgttgca cttaagcgat ctgttccgtc attccgcgca
     1561 gcaaatgggc gttacggcag gcctgacgct acccattttc gatagtggcc gtcttaacgc
     1621 caacctcgat atcgcaaaag ccgaaagcaa cttgtctatc gccagctaca acaaagcggt
     1681 ggttgaagcg gtgaatgacg tggcgcgggc agctagtcag gttcagacac tggcggagaa
     1741 aaaccagcat caggcgcaaa ttgagcgcga tgccttgcgt gtagtaggtc ttgcgcaggc
     1801 gcgctttaac gcgggcatca ttgcgggttc ccgggtcagc gaagccagaa tcccggcgct
     1861 gcgtgagcgg gccaatggcc tgttattgca agggcagtgg ctggatgctt ccattcaact
     1921 cactggtgcg ttgggcgggg gttacaaacg ttgatgatat gcgccttcta tacttaacgt
     1981 ttattcagcg ttaagtggag aactcgatgg cacaggtcgc gattattacc gcctccgatt
     2041 cggggatcgg caaagagtgc gcgttattac tggcgcagca gggggttgat attggtatta
     2101 cctggcactc tgatgaagaa ggggcaaaag ataccgcgcg tgaggtggtt agccacggcg
     2161 tacgtgcgga gatcgtgcaa ctggatctcg gcaatctgcc agaaggtgtg caggcgctgg
     2221 agaaactcat tcaacggctg gggcgtattg atgtgctggt gaataatgcg ggcgcaatga
     2281 ccaaagcgcc gtttcttgat atggctttcg atgagtggcg caagattttt accgttgatg
     2341 tcgatggtgc atttttatgc tcgcaaattg ctgcccgcca gatggtgaaa caagggcagg
     2401 gtggtcgcat catcaacatt acgtcggtgc atgaacatac gccgttgccg gatgccagcg
     2461 cctacacagc cgctaaacat gcgctcggcg ggttaaccaa agcgatggcg ctggagctgg
     2521 tcaggcataa gattttggtg aatgcggttg cacctggggc gatcgccacg ccaatgaacg
     2581 gcatggatga tggcgacgtg aagcccggcg cggagccttc gattcccttg cggcgttttg
     2641 gcgcaacgca tgagattgcc agcctggtgg tgtggctttg ttcggagggc gcaaattaca
     2701 ccactgggca gtcgttgata gtggatggcg gctttatgct ggcgaatccg cagttcaacc
     2761 cagaatagcg cagtgaggtg cgtggatatt tcattgggtt aggccagata agacacgcca
     2821 gtgtcgcatc aggcatggaa cacccggtgc cggatgtggc gtgaatgctt atctgtggca
     2881 actgctttac gcctgattat ccggcttttt cttcccgcgt cgtttcagcc accagcgcac
     2941 gcctaccacc agaaccacga ccagaatcaa ccagacccag tgcttcaaat gctggtcgag
     3001 attgtgcaac cacggcgcaa tcacctgacc accagcgtaa ccgatagtgg taaaaatcaa
     3061 cgcccaggca aatgcgccaa ggatattcag tggcagaaag attttcggcg gcagctggct
     3121 ggcaccaatc agcgtcgggc caatcaccct aaagccatac ataaaacgcg taccaatgac
     3181 aaacagatac ggatggcgtt ggataagttt ctgcgcccgc tcaattttat cctgatgttt
     3241 cgagaaacgg cgtaatagct tgccgccaaa gcgccgcccg cacagataaa gcacctggtc
     3301 gccaatcatg ccgccaagcg ccacagaaag taccaccagc gggaacttta atagcccctg
     3361 atgtgccgca acgcctccca gcaaagtcac ggtttcacct tccgccaggc taccgatcac
     3421 cagcgcggca taaccatatt gtgagatgag tgtattgaga tccataggcc agtctgtctc
     3481 cctttgcttg cctgtaagca tacaccctgt gaatagttca cgctgaaaaa ggacgtacat
     3541 cagacggtgc aaattacatg agcgaacaaa aaataatcac aagctgactt atacttgaac
     3601 tgtcaggttc atgaactcac aacaaggggg cggtatgagc catgtctggg gactgttttc
     3661 ccatcccgat cgtgaaatgc aggtgattaa tcgcgaaaac gaaacgattt ctcatcacta
     3721 cacccaccac gttttgctga tggcggcgat tccggtgatt tgcgccttca ttggcaccac
     3781 acaaattggc tggaattttg gcgatggcac tatcctgaag ttatcctggt ttaccggact
     3841 ggcgctggct gtcttgtttt atggcgttat gctggctggg gtcgcggtga tggggcgggt
     3901 catctggtgg atggcgcgca attatccgca gcgtccgtca cttgcgcact gcatggtctt
     3961 tgcgggctac gttgcaactc cgctgttttt aagcggtctg gtggcgcttt atccgctggt
     4021 ctggttgtgt gcgctggtcg gcacggtggc gctgttttac accggctacc tgctttacct
     4081 cggtattccg agcttcctga atatcaacaa agaggatgga cttagcttct ccagttcgac
     4141 gctggcgatt ggcgtgctgg tactggaagt gctgctcgcc cttaccgtta tactatgggg
     4201 ctacggttat cgtttgttct agaaagttct tttgtgttgc attgctggcg taaatgtcag
     4261 caatgcagca tttcctcacg attctccttt gacgatcgca ctttttgctc gttatgatgg
     4321 cgtcgctagc ctcagtaaat ccttactccg gcggtgcgca acccgtgcgc gtgaaccact
     4381 atatgaatgc tcatcatgcc gaaatttcga gtttctttat ttagcctggc cctgatgctg
     4441 gctgtgcctt ttgcaccgca ggccgttgcg aaaacggcag ccgctaccac cgcttcacaa
     4501 ccggaaattg cctccggtag cgcgatgatt gttgatctga ataccaacaa agtgatctat
     4561 tcgaaccacc cggatctggt gcgtccgatt gcgtctatca gcaaattaat gaccgcgatg
     4621 gtcgtgctgg atgcacgact gccgctggat gaaaaactaa aagtggatat cagccagacg
     4681 ccggagatga aaggggtcta ttcgcgcgta cgactgaaca gcgaaatcag ccgtaaagat
     4741 atgctgttgc tggcgctgat gtcttcagaa aaccgcgcgg cggcaagcct tgcgcaccat
     4801 tatcccggtg gttacaaagc ctttattaag gcgatgaatg cgaaagcgaa atcgctcgga
     4861 atgaacaaca cgcgctttgt tgaaccgacc ggactatcgg tgcataacgt ttcaactgcc
     4921 cgtgacttaa ccaaactgct cattgccagc aaacaatatc cgttgatcgg gcagttaagt
     4981 actacccggg aagatatggc aaccttctct aacccgacgt atacgctgcc gttccgcaat
     5041 actaaccatc tggtgtatcg cgataactgg aatatccagc taaccaaaac cggctttacc
     5101 aatgcggcgg gccattgtct ggtgatgcgt acggttatca ataataaacc ggtggcgctg
     5161 gtagtgatgg atgcgtttgg caaatatacc cattttgccg atgccagccg cctgcgtacc
     5221 tggattgaaa ccggtaaagt gatgcctgtt ccggcagcag cgttgagcta taaaaaacaa
     5281 aaagc
//
LOCUS       DAOTRK010000228         5276 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.230, whole genome shotgun sequence.
ACCESSION   DAOTRK010000228 DAOTRK010000000
VERSION     DAOTRK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.230"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1276)
                     /gene="aroA"
                     /locus_tag="QIE41_004481"
     CDS             complement(<1..1276)
                     /gene="aroA"
                     /locus_tag="QIE41_004481"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDS3533264.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALAALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGSFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQ"
     gene            complement(1347..2435)
                     /gene="serC"
                     /locus_tag="QIE41_004482"
     CDS             complement(1347..2435)
                     /gene="serC"
                     /locus_tag="QIE41_004482"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDS3533265.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNIPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2634..3326)
                     /locus_tag="QIE41_004483"
     CDS             complement(2634..3326)
                     /locus_tag="QIE41_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDS3533266.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3456..5216
                     /locus_tag="QIE41_004484"
     CDS             3456..5216
                     /locus_tag="QIE41_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HDS3533267.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1335 a         1363 c         1269 g         1309 t
ORIGIN      
        1 cctggctaat ccgcgccagc tgctcgaaat aatccggaaa tgttttggcc gtgcatttgg
       61 gatcaagaat cgtcactggt gtatctgaca acgccaccag cgagaaacac atcgccatcc
      121 ggtgatcatt gtatgttgcg atctcggcaa agttcagttt ttccggtgga gtgatacgaa
      181 tgtaatcgtg cccctcttct acttccgcac caactttacg cagttctgtt gccatcgcaa
      241 acaggcgatc ggtctcttta acacgccagt tatagatatt gcgcagcgtg gtggtgcctt
      301 ttgcaaataa cgccgccgtg gcaatggtca tcgccgcatc aggaatatgg ttcatatcca
      361 tatcaatagc gttcagttca ccccgcgtgc aggaaatata atcatcgccc cagcaaatgg
      421 tcgcgcccat tttttccagc acatcagcaa agcgaatatc accctgcata ctgttgcgtc
      481 caatgccggt cacttttaca gtgccgcctt tgattgctgc tgctgccagg aagtaagaag
      541 ccgaagatgc atcgccttcg accaaataag tacccggaga ctgataagac tgcccgcctt
      601 ttacgacaaa ttgttgatag tgctgatttt caatttcaac accaaacgtc ttcatcagat
      661 tgagtgtgat gtcgatataa ggtttagaaa ccagatcgcc tttaatacga ataaccgtat
      721 cttccggcgc aagaggcgca gtcattaata gtgcggtgag gaactggctg gaaacggagc
      781 catcaacgtc aacgttgccg ccagtaaagc tgccctgtaa acgcaatggc ggataatttt
      841 cttgttccag gtaagtgatc ttcgccccgc ccaggcgcag cgcatccacc agatgaccaa
      901 tcgggcgctc tttcatacgc ggctcaccag tcagcacaat atcattgcta cccagacaaa
      961 gagctgccgc cagcgggcgc attgccgttc cggcgtttcc gaggaacaac tccagggcac
     1021 cttctgcgtg taatggaccg ccgttaccga taatttcgca acgcgtacga tcagctgaaa
     1081 gcgtatagct tacccctaac gctgctaatg cattcagcat atggcgcacg tcatcgctat
     1141 ccagcagatt ggttaatact gttttgccgt gtgctaatgc cgccagcaat aaagcgcggt
     1201 tagaaacgct cttggaaccg ggcagattaa tagtgccatc gacacgagcg atgggttgta
     1261 acgtcaggga ttccatgaac tcaactctct acaacagaaa taaaaacccc acaggctggc
     1321 tgtggggatt aagcaaaatt tcggcattag ccgtgacggc gttcgaactc aaccatgaag
     1381 tctgtcagcg ctttaacgcc ttccagcggc atggcgttat aaatagatgc gcgcattccg
     1441 ccgaccacac ggtgaccttt cagcgcatga aggccagcag caaaagactc ttcaaggaac
     1501 aatttgtcaa gcgcactgtc cgccaactgg aacggcacgt tcatccgcga acggttagct
     1561 ttcgccacgt cattgcggta gaaatcgctg ttatcaatca ccccatacag cagttctgct
     1621 ttttgctgat tgattttatc cattgcagct acaccgccgt tcgctttcag ccatttaaag
     1681 accagaccag acagatacca ggcaaatgtc ggcggcgtgt taaacatgga gtcgttatcg
     1741 ttgagaatgg aataatccag aatcgacgga cacgcgatat tcgctttgcc cagcaaatct
     1801 tcacgaacga tgacgattgt caggccagcc gggccgatat ttttctgcgc gccagcgtaa
     1861 attacaccat aacggctgac gtcaatcgga cgggaaagaa tggttgaaga gaagtcggcg
     1921 gcgaccacca catctttgcc gaagtctggc gtttcgtcga tagcgatacc gtcgatggtt
     1981 tcattcgggc agtaatgcat ataagcagca ttatcagaga gttgccattc gcgcattggc
     2041 ttaaccgcgc gcagaccatc aacagtcact ttggcgtcga agacattagg cgtgcaatat
     2101 tttttcgctt ctttaatggc acttgccgcc cagtaaccgg catcaacata atctgcggtg
     2161 gttttatcac cgagaatatt cagcggaaca gcagcaaact gaccgcgacc gccgccatgg
     2221 cagaacaata ctttgtagtt ggaggggatg ttaagaagat cgcgaaaatc cttctcggct
     2281 tcctctgcaa cctgaatgaa ctctttgccc cggtgactca cttccatcac cgacgtacca
     2341 agaccgttcc agtcacgcag ttcctgttga gcctgtttaa gcacctctgc cggtagcatt
     2401 gccggaccag aactaaaatt gaagatttga gccatttccc ctcaccacgt tgcgttgcta
     2461 ttaacccccg gtcaatcgat tgcgaccgcg ggtaatatgt tattcctgtc gctatcggtt
     2521 ttatcattca gtgacacgca tcgcaatggg taaaagcgtt gagggcaaaa atgcggcacg
     2581 cgtcacacaa cagaatctct cggattatta ccaaaaaacc gatatttttg ctgtcaggtc
     2641 acccgtttag ctcgacttgc cttcgtccat tctggatttg cacagcgcgg acataattgt
     2701 ttttcccccg cgttcatatg aatgatttca ctgcaacgga tgcaggcata gtccgccgac
     2761 tccggcacca ctttgtaacg ctgagtacag tcactgggta aaataagtaa gcccggtttc
     2821 accttgtcat cttcaaagaa ataaacactg atttgcccgt tggtttcgag aatcgccaga
     2881 cgtacttgcc ccaactgctc tacaccgcgc aatcgtagct ccataaagaa ctcaaattcc
     2941 gtcatgttgg agttattgag tttcgaccag gccagctcgc catcttcaat aatgacaacc
     3001 ggcttacctt ccagaagatc ttccagtttt tcactgtgtg ccatcaacca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg ggaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgacccc agcgtcagaa tgattaacac ttcaaacagc
     3181 gacatctgcc gcactccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtatacagac tacgtagtgc cacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcatttgt atgctcttat cgtggtttat ccgctaaaag
     3361 catagtcaac agatcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc actggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaaa aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc cgttcgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctgcaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agccatcgaa acgctggaag cagaaggttt cccaatcttt gcttatgatg
     4201 gttcgcttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gctaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agttgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaggaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgaatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctggc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgttaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcgcag gaagaagatc gccagccgct gcaatatttg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtggaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttatgg cttgcaacca gtagatagcg atctgcacgc gtttgcagca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tcgtctgagt tatattacgg ggcgtt
//
LOCUS       DAOTRK010000229         5164 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.231, whole genome shotgun sequence.
ACCESSION   DAOTRK010000229 DAOTRK010000000
VERSION     DAOTRK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.231"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1309..1518
                     /locus_tag="QIE41_004485"
     CDS             1309..1518
                     /locus_tag="QIE41_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533268.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1584..2117)
                     /locus_tag="QIE41_004486"
     CDS             complement(1584..2117)
                     /locus_tag="QIE41_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533269.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHGNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2120..3046)
                     /locus_tag="QIE41_004487"
     CDS             complement(2120..3046)
                     /locus_tag="QIE41_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDS3533270.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSG
                     NLTPTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQDRTEPERVKSPSRGFSMGF"
     gene            3223..3621
                     /locus_tag="QIE41_004488"
     CDS             3223..3621
                     /locus_tag="QIE41_004488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533271.1"
                     /translation="MRISNIEWLKKRIRFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3825..4739)
                     /locus_tag="QIE41_004489"
     CDS             complement(3825..4739)
                     /locus_tag="QIE41_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDS3533272.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1438 a         1346 c         1107 g         1273 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacac agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaacga caagttatac aataaaacca gtactatcca
      781 tacagctaat tacatataaa tactattaaa ccatctggac attaaaaata aactcttata
      841 acataaaatt aatgcggttc cggtaccata tgtcagggat aatgtgcaaa tggaggtatc
      901 ggaactgcat ttcagttcat tattaattaa tgacaaaaaa aacctctgcg agcgcagagg
      961 gggtaaggaa tgttcccggt cataatatgt cgatagtgtt tatatacata cagaacaaga
     1021 acaacaacca gccaactaat aaatatttta ttttttagag ctgaaacctt aaagctcact
     1081 tccattccat acattgttat aaaccttatg ttgaaaactg aaagtaagta cggcagactt
     1141 attaagttgc gtgcagtctt ttcacgttat acaaacaaga acacctgcga agttatttct
     1201 ccttacatat ccccctatgc caacggggga ttttttctgg ttcactaacg cataaataat
     1261 agtgacaccc tatcattcac aaaccgtgat aatataagga ttatcatcat ggcaaaatta
     1321 ggtgaaaaca ccccacatat tatcgatgct actgtagatt ttatggcatc aagccaggca
     1381 tttcgtgagt atctgaaaaa acttccccct gataacacaa taccgccagc tcttactgat
     1441 gagcacgtac cgttatatat acgaaggctg gaatactatc gactgcttta tcgaccaaag
     1501 cagacagaat catattgaaa aaaacggtga acggttcgcg aaatcctttc gcgggtcgtg
     1561 aggcgttgga caagcgaagc gcgtcagttt ttccggctct tccccgtgac aggtttctcc
     1621 accagtttat cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc
     1681 ctgtcgagta ttgttttcat tccccatccg agcacacaga acataaccag aaaagccact
     1741 gacaccccac ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata
     1801 ttgccgtgaa ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg
     1861 actgactctt ccagccgctg aaaatcctgg ctgatgcgct cacctatcag tcctgccacg
     1921 tccagcttct gcacctcatt tctgacttca tccctgacct gttttgccac accgtcagaa
     1981 atatcccgat atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc
     2041 tgttgctcca ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt
     2101 tcttctgtgc tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc
     2161 gttcaggttc agttcggtcc tgtctgctca ctctgtctct catgtcaaag ccatgctccc
     2221 gcgcaacatc atgttgaagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc
     2281 attccctgat gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt
     2341 cagaccgttt ttgcatgatg tcatgacgct gggcatcatc aagccgtcca gcagcttcaa
     2401 aacccagatg cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa
     2461 tgaagcccct ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt
     2521 ttcgcaattc ccgcaactgc tctttttgct gagtgggggt caggtttccg gaatcctttt
     2581 tcgcacctcc ccggtcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac
     2641 gttcaatgcc atcattcaca cgctgactca tcatgatatg cgcatgaggc tgttcgccgc
     2701 cgtcaatgct cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt
     2761 cctgacgtac aaaatcctgc accagttcaa ggcgctgttc cggtgtcagt tcacggggta
     2821 atgcaatttc aagttcccta taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct
     2881 gccagaaatg ggaaggatta tccctggccc attccggcat gtttccgtgt gcggaatatt
     2941 ccaaatcctg acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag
     3001 cctgcccctt gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctct
     3061 gcaggagcgc actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt
     3121 ttacgaatta gcacggaaga taccccctgc acccgccgac cgcaagcgat atactgcacg
     3181 accacaacac accgcaaaaa aacaccaggg agcatcacga acatgcgcat cagcaacatc
     3241 gaatggctga agaaacggat cagattcatc aggaagctcg gagaacagac cgcacgacag
     3301 agacagatta ttgacctgat cgacaacgaa gccggactca cagaacagga acgcaaactt
     3361 ctccacgtac tggcaacagc ggaaaaaaac gacttacagg cacaggagag cgaacgaaaa
     3421 caggcggtcc aaaaacgaat cgagggaaaa aaacaacgac gggaacgcaa tcaccggctc
     3481 tttctcgcgg ctgggctact gatcgaagca ggactggtag acacaaaaac aggcgagcta
     3541 tgctataaaa aagacaggat attacaagca cttaaggaac ttaagtacga tctggagaca
     3601 tccccaaacc cagacgcgta agtgcctcca tccgtgttcc gattccctgg tgcttcctcg
     3661 ctcattcgct cgctacgctc ggtcatgaga ctgcggcgag cactaccacc acaatcacgc
     3721 ggaaagccgc gccattgcag ggctgaacgg ggaaaaacgg caaaaaacct gctgctcaaa
     3781 aagacgccag aaaggcgctg ttatctgata taggcttatt tggtctactc tacgagacct
     3841 cgttttttca gttggtaata ccaggcgcga ctgatcccta atgcctccca cggtttcagc
     3901 gtgcgtgcag atgtagctac tgtgctgcgc ttagacttag aaccgccctt gcgaccgcgt
     3961 gcagcctgaa tttctggcgt gtgcgtatca gccacatact gtgcgaaagt ttccggcgtg
     4021 aagttcctgt gcgtgtattt cgcaatgctc ttgccaatag cccgacattc agccagtgaa
     4081 agcggaacgg gaagcgatgc attgtacatt tcgacacgct ggatcaccgc ttcaagccac
     4141 tgtgagaacg ctggccagcc ctgacgaatc gccctgtaag cccatttgcg cgtcatttcg
     4201 aacaggtggc agtttcgccc catcccgtaa tgtttatcga tgctacgacg ctctgaggcg
     4261 ctcaaatcga gataatcagc cagttcatcg agggtatagg cttcctcgcg ccactccatc
     4321 accagccatt ccaggtggaa cggatttttg caaatcaggc cgctgtagtt cacatccgca
     4381 caaagtttct cacacagcga acgttcaacc gctgcggcat atttcagcgc cttaaccgag
     4441 gcatcaggcg cggttctcac ggcgatgttg agtgcgtaga gcaggtgagc gtgtccgttc
     4501 acaggatttt taacggtgat gttcggcgcg ggagcgttcc ggtcgctcca gtcaatcgca
     4561 gccccctcgc ggtcaacgtc aaaaaccagc cagaactgtg catgaggctg gttctgctgg
     4621 atataccgtg caagaagcgc gcgccctttg ccggagatac gaaggccgaa agcgagatca
     4681 tccgtgtgat agggacggtg gggtaaattt tcttcgaagt attgaagcgc ggcgctcatg
     4741 tgttacgatt ccttccaggt tatcgaccga catcgcccct gtattttcgt gaaagtcttg
     4801 gcggatagac acgaatttca ggggcgtttg tttttcagtc gcttacacta cacccaacgg
     4861 caaatcctgt cgatccttcc cgaaaaaccg atccatttac cataactccc gttgtacgta
     4921 ccccttaaaa cgccctcagt cgctttctgg cgcaaaaaac gcaaaaagac aagaaaacac
     4981 caggccaaac cagaaaaacg cgccagaatc gtttttagag ggctgcataa ctatgcacga
     5041 aagcgatgtg cgatcggttt cgtgcgttct gaacaccgaa cggctcaaaa cgacaagttt
     5101 tctcccctgg tgcgtggcgc tctgacgggg tttcctgttt ccaaacttca catctcgcca
     5161 ggac
//
LOCUS       DAOTRK010000230         5092 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.232, whole genome shotgun sequence.
ACCESSION   DAOTRK010000230 DAOTRK010000000
VERSION     DAOTRK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.232"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            14..349
                     /locus_tag="QIE41_004490"
     CDS             14..349
                     /locus_tag="QIE41_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDS3533273.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(435..1283)
                     /locus_tag="QIE41_004491"
     CDS             complement(435..1283)
                     /locus_tag="QIE41_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDS3533274.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFIGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            1632..1829
                     /locus_tag="QIE41_004492"
     CDS             1632..1829
                     /locus_tag="QIE41_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533275.1"
                     /translation="MQGAPHPARLKKNDGTTMACGGHWHTTPAQVKTEKTARHFQPKK
                     VKFLHFFTLIFRADLVIMVIK"
     gene            1863..2114
                     /locus_tag="QIE41_004493"
     CDS             1863..2114
                     /locus_tag="QIE41_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3533276.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(2145..2351)
                     /locus_tag="QIE41_004494"
     CDS             complement(2145..2351)
                     /locus_tag="QIE41_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533277.1"
                     /translation="MTIAERLIQKGFDEGFDEGFKEGFKKGALEVAREAACRLRDMGW
                     TPERIQEAAGLSGEELKKLFPDEQ"
     gene            complement(2413..3315)
                     /locus_tag="QIE41_004495"
     CDS             complement(2413..3315)
                     /locus_tag="QIE41_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDS3533278.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGALEKALAIACQLQK
                     MGMTPEQIKQATGLSDDELKKITH"
     gene            complement(3312..3701)
                     /locus_tag="QIE41_004496"
     CDS             complement(3312..3701)
                     /locus_tag="QIE41_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HDS3533279.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(3878..4144)
                     /locus_tag="QIE41_004497"
     CDS             complement(3878..4144)
                     /locus_tag="QIE41_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533280.1"
                     /translation="MKPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(4238..4672)
                     /locus_tag="QIE41_004498"
     CDS             complement(4238..4672)
                     /locus_tag="QIE41_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HDS3533281.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(4916..>5092)
                     /locus_tag="QIE41_004499"
     CDS             complement(4916..>5092)
                     /locus_tag="QIE41_004499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533282.1"
                     /translation="LKNQACSDRSGRAAPVAPETALPAHLTVCKATAPDGAVNPSAAT
                     PAACFPLWTFRGLE"
BASE COUNT         1197 a         1378 c         1314 g         1203 t
ORIGIN      
        1 agagaaagtg ccaatggctt ctaaaaaatt ttactcagat gatgacatcc agctggcaaa
       61 agctgcttta tcggaattgc cggacctcac cgctcaacgg aaaacattgc gtgattttct
      121 tgatgccatt cgtgatgaca tcattatcct ggtaaggaca aagggttata cactggcaga
      181 tgttcgtgac acgctcaaaa atgcagggta tgaagtagga gagaaggcac ttcgtgacat
      241 tattcgggaa gcagaatcga aaaaaccatc ccgccgttct tcctctaaaa cagcacctaa
      301 aaagacagat tcaggcagga aagatggcat cgatatgaac aacaattgat gtgacgatgt
      361 cacatcgtgt tctgagatta tgatgattac agaaaaaaaa gccccggcat aaataccagg
      421 gcttttctgt ttaatcagac tcgtggcggc aatgcacctg gataatgccg tgcaataata
      481 tcattcagtt tttcaacaag gtcagaacgc ttaaagacaa tgtgtacggt ccctttctgg
      541 tagccacgca ccgtaaaata ttccccttct aacagcttac cgacattgtc gccgttcccg
      601 ataaattcac catactgcgc cccgatgctg ttcctgaagt caggaacgtt gcgcccctcc
      661 agcagataaa acggacgtac caggtcatcc agtgcctccc gtccgctgca gtccagcgtc
      721 acacgcccat tactccagcg gtacagcagg ttttcaataa tgattttctt acccaggcgg
      781 cagggattat tggttctgta atcccatgac aatttccggt agacatcaat cagaccttgt
      841 tcaaacgtct gcatcttgct ggcattaaga tggcggaatg ttgccagtac attgtccagg
      901 gtgatttcag gacaggtgtc gctcatcagc tggctgttcc actcatcacg ctggcatgaa
      961 ctcatgaagg tgtacatgcc cgtatctgtc atcaggcggt cccagatttt ccggtcggca
     1021 agacgggtga tattcctttt cgcttcttcc ggtttatcca tgcgacgaac ggaatcagtc
     1081 acgcagtctt caaacccgta aagaaaaata tcacctgacg catcgagcag aatcctgcgc
     1141 gctgtcgcca ggcattccat cgcctgcatg aacagtgcaa taccttcgtt acgctgcgcg
     1201 atgatgcggt cgatggccac agacggaata acatctgtcg tgccggcagt attcaggtca
     1261 gcagtgggta aagtctgatt catcacattg tctcctcatc gtcacgactg ccgggtcagc
     1321 caggtcattt cccggaggag cgaagctcct tctgccgtcc gggtcttcgg ggatttcccc
     1381 gcagtggacc tttccgacgg gggacgctca ccacgtcacg gggcagttgt ccgggggcag
     1441 acgcaaaagt ttttgacgcc tgcggcaaaa agttttgtgg ctggtacatc tctcccctgg
     1501 atgactgaaa cgggatgtgg tcgtgtatat acccccggaa agtccgtctg cggagaacag
     1561 gccgcagtgc ggcggcagac aggcagagac agctgcgcca tcaggcgcag tggccttaca
     1621 caccgtaagg tgtgcaggga gcgccgcatc cggcgcgact gaagaaaaac gacggcacaa
     1681 caatggcatg tggtggtcac tggcacacca ctcccgcaca ggtgaaaaca gagaaaaccg
     1741 cacgacattt tcaaccaaaa aaagtgaaat tccttcactt tttcacactt atttttcgcg
     1801 ctgatttggt gattatggtg ataaaataaa gtgaagtttt ttcacttttt caaaggtatt
     1861 ttatgaccgg ctgggaactg cgaatctggc gaaaaagcat gctctggtca cgcgaaaaag
     1921 cggccagaga atttggcgtg acgcagagaa catggcacgc ctgggaaaat gcggaacagg
     1981 tggacgtcac cgtctggagg accacccagg cactttcagt ccgtgacctg ctgcctcaca
     2041 tgcaggggat gcgcaaggca gatatcatca ggcgtctgga aaatgaactg ggggaaacgg
     2101 cagaggatgt gtgagcgtga ttcacagcct ggcagaatgc caggctactg ctcatcagga
     2161 aacagttttt tcagttcttc accggaaagt ccggctgcct cctgaatccg ttccggcgtc
     2221 cagcccatat cccgcagccg gcaggctgct tcccgcgcca cttcaagcgc acctttctta
     2281 aaaccttctt taaaaccttc gtcgaaacct tcgtcgaaac ctttctgtat aagacgttct
     2341 gcaatcgtca tactgttttc tctgctcctg ctggcaaaag gcccggtatt ttgccgggct
     2401 ttgtggttct catcagtgag tgattttctt cagttcgtca tcggaaagtc cggtagcctg
     2461 cttaatctgc tccggcgtca tccccatttt ctgaagctgg cacgcaatag ccagggcttt
     2521 ttcaagcgca ccttccatcc tgccctcctg gcgacctttt tgctcaagac gttctgcgat
     2581 agtcatcagt ttatcctcat gctgcggcag acgctcagcc agcgcccgca taaattccgg
     2641 tgatacagag tcccctgcgt taacgatgta atgaatcaga acacgcacct gctcgtctga
     2701 aatttccacc atgacctgcg ggagtttgtc cagcagctcc atcatgtcac gatggcggat
     2761 atgcttcatc agcagcgtca gtgcggccat ccggcgatgg ttcatgattt cattgtcatc
     2821 aacgacagtg atatcaacca gcggaaacgg ctttgtgtat attttagccg caagcgccgg
     2881 attctcaaaa cagtccagcc agttcatgct gtacggatac gggctgcgct caccatgata
     2941 gaacagcacc ggaatcacca gtggcaacgt tttgtgctgc tccagatgtc tctgcatcgc
     3001 cgccacactg taacgtaaca tgcggaaagg catgtacaga ttttcggtgc tctggtgctc
     3061 gatgagcgta taaacatagc cgtctttacc cccggtggtt ttcacgctcc agaggatatc
     3121 gctggcgtac tgatgcaggt ccggctctac aaaggtggcg ggttccagct tcagcgtgga
     3181 caggtcgcac aactgccggt actccgcagg aagatgcagt tccagaaaat ctctggcgac
     3241 gtcgggattc gccaggaacg accggaacgc ggcatcatgc ggcgtgggcg tcgtggtgtt
     3301 tttcttctta ctcacggttc ctccatcaaa tcagcagcat cgcagcaatc atcgcccaaa
     3361 tccccacaac cgagaggaca gcaattgcgt tcaaagccat gacaacaata gccgcgttct
     3421 gagtcacggc gtatttcttc agcaatctct tctgcggaaa ggtaatcaaa ctcctcacgg
     3481 gccagttcca ggaccagcag atgttgttct gtcggcaatg tggccacaaa attatcaata
     3541 gcagcactca aacgtcgctg atatagctca taaacaagac cacaacgggg cattagttct
     3601 tcggataata gttccagttt tgagcgaatt ctgtcagcgt tgaaagtgac ttcatcaaaa
     3661 gacatatgaa aagctttgtc agtatcaaaa caagacttca ttgttatctc cagccctccc
     3721 gggccctgaa tgaaagagaa tctgtttccc ttttctgttt tttgtggaga caccatgcaa
     3781 cacagacgtc gtgatgtttt tcatcttgct cacggttcct cctggtaaag tacctgaaca
     3841 catcctgcca cctgcacagg cagacggcag gggttaatca gtgacagacc cgttcaattt
     3901 caagcgcgcg gagcaggtgg ccccattcgc tgactttcat ggcggggata cgtacccgct
     3961 tctgctcact gcagcgtctg gcgatatgac cgctcatcgc caggacgtcc gggttaatct
     4021 catcctgtgc cagctcctga cgtctgaagc gggtatggcg gtgcaggggt ttattctccc
     4081 gcaggcactc ttcacgcaga cgctttacat actcggcggt tgcgaagata attgatggtt
     4141 tcatggcata atctccatag atagtgattg agatgccggg cttcctggcg gggaagcgcc
     4201 ggcggagaga ggcagggttt atcgccctgc ctttttttca tttcggacag cggcgcagtg
     4261 ccagcgcgag cgctttcatc ttccagcctt ccggcgtgcg atacatcacc ttccagtagc
     4321 cgtcgatgaa cacataatgg aagcgcacct cttcagtgct ttcagtggca atagcctcca
     4381 tgaaagaacg gtactcacgc gcctcactgt ccatccggaa accactgtca cgtccgtaat
     4441 aagccgtcac accgtccacc gggttctgaa gcgtatgccc ggcgggtttg tcgcaacggg
     4501 gaccgaggca acggatatca ccgtgttcaa ccagggcttc ggcctgctga ccagtggcat
     4561 agtgggtatg caggatgcga ccgttccagg tcggctcacc gtgcaggtgg cagtaaacgg
     4621 tacggaacac cccgtcgcgg caaaaaacgg aaatcgtgct gctgactgtc atgaaaacgt
     4681 ctccttctgc tgaatcaggt cgccgggccg accggttgct ccccggaggg gcgaaagccc
     4741 cttctgccgt ccacttcttc ggggtttctc cccgcagtgg acctttccga tggggcgtcc
     4801 gtcaccacgt cacggggcag ctgtccgggg gcggacgcaa aattttttgc cgggtttatg
     4861 gctaaaaatt ttgtggacgg tatatcttcc cctggactgc tgaaacggga tgtggttact
     4921 ccagcccccg gaaagtccac agcgggaaac aggccgcagg ggtggcggcg gaagggttga
     4981 ctgcgccatc aggcgcagtt gccttacaca ccgtcaggtg tgcagggagc gccgtttccg
     5041 gcgcgacagg ggcggcgcga ccggaacggt cgctgcaagc ctggtttttc ag
//
LOCUS       DAOTRK010000231         4944 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.233, whole genome shotgun sequence.
ACCESSION   DAOTRK010000231 DAOTRK010000000
VERSION     DAOTRK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.233"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(24..2897)
                     /gene="gcvP"
                     /locus_tag="QIE41_004500"
     CDS             complement(24..2897)
                     /gene="gcvP"
                     /locus_tag="QIE41_004500"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDS3533283.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMSGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(3015..3404)
                     /gene="gcvH"
                     /locus_tag="QIE41_004501"
     CDS             complement(3015..3404)
                     /gene="gcvH"
                     /locus_tag="QIE41_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDS3533284.1"
                     /translation="MSNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(3428..4522)
                     /gene="gcvT"
                     /locus_tag="QIE41_004502"
     CDS             complement(3428..4522)
                     /gene="gcvT"
                     /locus_tag="QIE41_004502"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDS3533285.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLHGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            4813..4896
                     /locus_tag="QIE41_004503"
     CDS             4813..4896
                     /locus_tag="QIE41_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533286.1"
                     /translation="MSKNTKSKIRGIRKYNAKSQVKLVYFK"
BASE COUNT         1178 a         1355 c         1254 g         1157 t
ORIGIN      
        1 tataagatag tcgaatcagt aaattactgg tattcgctaa tcggtacgca ggagcagaac
       61 aggttacggt cgccgtaaac atcatccaga cgtttcacgg tcggccagta tttgtctgcc
      121 acacctgccg ggaatactgc aacttcacgg ctgtacggat gcgcccactc ggcgaccagt
      181 tcgctttgaa tgtgcggcgc attcaccagc gggttatctt ccagcggcca gacaccggct
      241 ttcacctgat caatttctgc gcggatagcc agcatcgcat cgataaagcg atccagttcc
      301 actttgcttt cagattcagt cggttcaacc atcagcgtac ccgccaccgg gaacgacatg
      361 gtcggcgcgt ggaaaccgta gtcgatcagg cgcttggcaa tatccagctc gctgatgccg
      421 gtttcttctt tcagcgggcg aatatcgaga atacattcgt gcgccacgcg accgtcgcga
      481 ccggtataca gcaccgggaa ggcatcctgc agacggctgg caatatagtt ggcgttaaga
      541 attgccacct ggcttgcttt tttcagccct tctgcgccca tcatgcggat gtacatccag
      601 ctgattggca ggatagaggc actaccgaac ggtgccgcag aaaccgcgcc ctgacgggtt
      661 aacatgcctt cgatttgcac cacgctatga cccggtacaa acggagccag atgcgctttc
      721 acaccgatcg gtcccatacc cggaccacca ccgccatgcg gaatgcagaa agttttatgc
      781 aggttaaggt gggaaacgtc cgcaccaata aagcccggcg aggtgatgcc aacctgagcg
      841 ttcatgttcg cgccatcaag gtaaacctga ccgccgaact gatgcacgac ttcacacact
      901 tcacggatcg tttcttcata cacgccgtgg gtagaaggat aggtcaccat gatacaggag
      961 aggttatcgc ccgcctgttc cgctttcgcg cgcagatcag tcagatcgat gttgccgttt
     1021 ttatcacacg caacaaccac cacctgcatt cctgacatat gcgcggaagc cgggttagtg
     1081 ccgtgcgcag aagccgggat caggcagata tcgcgatgcc cttcgttgcg gctttcatga
     1141 taatgacgaa tcgccagcag gcccgcgtat tcgccctgtg cgccagagtt cggctgcata
     1201 caaacggcgt cgtaaccggt cagtttcacc agccagtcag ccagctgcgc gatcatctgc
     1261 tgataacctt cagcctgttc tggcgggcag aacgggtgca gttcggcaaa ttccggccag
     1321 gtgatcggga tcatctcggc ggcggcgttc agtttcatgg tgcaggaacc cagcgggatc
     1381 atcgcctgat tcagcgccag atctttacgc tccagcgagt gcatatagcg catcatttcg
     1441 gtttcgctgt ggtagcgatt aaacaccgga tgggtgagga tttcgtcgtc gcgcagcatc
     1501 gcagcctgaa tagagcgact gtcgtgagcc acgtctttgt ccagtgtgtc gatgtccagc
     1561 ccgtggttat cgcccaacag cacgctgaaa agctgcatca cgttttcgcg cgtggttgtt
     1621 tcatcaaggg tgatcccaac cgcgttcaga atatcgctac gcaggttgat ttcagccgct
     1681 tcggcacgcg ccagcacgcc cgctttgtcg gccacttcca cacacaaagt atcgaaatag
     1741 tgcgcatggc gcagcttcag acctttttgt tgcaggcccg ccgccaggat atcggtcaga
     1801 cggtggatgc gattagcgat acgtttcagg ccaaccggac cgtgataaac ggcatacagg
     1861 ctggcaatgt ttgccagcag tacctgggaa gtacaaatgt tggagttcgc tttctcacga
     1921 cgaatatgtt gctcgcgagt ctgcatcgcc atgcgcagag cggtgttgcc agctgcatct
     1981 ttcgatacac cgataatacg gcccggcatt gagcgtttgt attcgtcttt cgccgcaaag
     2041 aatgccgcgt gtgggccacc atagcccatc ggtacgccaa agcgttgcgc cgaaccaaaa
     2101 acaatatccg cgccctgttt acccggcgca gttaacagca ccagcgccat aatatcggcg
     2161 gcaacgctga ccacgatttt gcgtgatttc agttcgctga taagcgcggt gtagtcgtga
     2221 atttcaccag tagtgccgac ctgctgtaac agcacgccga agacgtcctg atggtcgagc
     2281 actttttgcg cgtcatcgac aatcacttca aaaccaaagg tttcggcacg agtacggacc
     2341 acatccagcg tttgcggatg aacgtcagaa gcaacaaaga agcggttggc atttttcagt
     2401 ttactgacgc gtttcgccat cgccattgct tcggcggcag cggtggcctc atccagaaga
     2461 gaagccgaag ccatatccag cccggtcaaa tccagcgtta cctgctggaa gttgagtagt
     2521 gcttcaaggc ggccctggga gacttcaggt tgatacggag tatacgcggt ataccagccc
     2581 ggattttcca gcatgttacg caggataacc ggcggtagct gcacggcggt gtaacccatg
     2641 ccgatgtaag acgtgaagcg tttattgcga ctggcaatag ccttgagttc tgccagtgcg
     2701 gcgtattcgg tcgccggtgc gccaacctgc ggtggtgtcg caagctgaat atctttcggc
     2761 acaatctggc cggtcagcgc gtttaacgat tgtgcaccaa cggcattcag catttcttgc
     2821 tgttgcgcgg cgtccggtcc gatatggcgt tcaataaaag cgccgctgtt ttcaagctgg
     2881 cttaacgtct gtgtcatgag cgatggttcc tgaaacgtgc agtgaattgt gaacctctct
     2941 ccttgcgaag agagtgaggg tgaggcgtaa atttcctcac cctgatcctc tcccgcagaa
     3001 gaggaataaa gccgttactc gtcttctaac aatgcttcgt atgcggttgc atccagcagt
     3061 gattccagtt cgctttcatc gctggctttg attttgaaaa tccagccgcc tgcatacggt
     3121 tcgctgttca ccagttccgg agaatcgctc agtgcatcgt ttaccgccac gatttcaccg
     3181 cttactggcg cataaatatc tgacgccgct tttaccgatt cggcaaccgc gcagtcatcg
     3241 cccgcgctaa ccgttgcgcc cacttccggt aggtcaacaa acaccatatc gcctaacagc
     3301 tcctgagcgt gttcggtgat gccaacggtg taagtgccgt cggcttcttt acgcaaccat
     3361 tcgtgttctt tgctgtattt cagttctgct ggtatgttgc tcatcaatca atctccaaaa
     3421 aagtaaatca cgcgacggct ttgccgttac gcacaaaaac aggtttcgtc actttaaccg
     3481 gcatttcacg gttgcgaatt tgcacaatcg ccgtttcacc aataccttcc ggcacgcgcg
     3541 ccagcgcaat gctataaccc agcgttgggg agaaagtacc gctggtgata atgccttcat
     3601 gctggttgcc ctgcgcatca gtaaaacgta ccggcagttc attacgcagc acgccttttt
     3661 cggtcatcac cagaccaacc agtttttctg tgccatgttc acgctgcgct tccagagctt
     3721 cacgaccgat aaagtcacga tctgccggtt cccaggcgat agtccagccc atattggcgg
     3781 ctaaaggaga aatagtttcg tccatctcct ggctatacaa attcatgccc gcttccagac
     3841 gcagcgtgtc acgcgcgccc aggccacatg gcttaacgcc cgcttccacc agcgcgcgcc
     3901 agaaatcggc cgctttttca ttgggcagcg caatttcgta gcccgcttca ccggtgtaac
     3961 cggtggtggc aataaacaga tcgcccgcct gcacgccaaa gaacggtttc atcccttcca
     4021 ccgcctgacg ctgggcgtca ttaaacagtg tggcagcttt tgcctgcgca ttcggccctt
     4081 gtacggcgat catggaaagg tcatcacgaa cagtaatttc gatgccgaaa ggttcagcgt
     4141 gttgggtaat ccaggagagg tctttttcgc gggtggcgga gttaacaacg aggcggaaga
     4201 aatcttcagt aaagtagtag acgatgagat catctatcac accgccagag gcattcaaca
     4261 tccccgagta aagggctttg ccgcttttgg tgagcttcgc cacatcgttc gccagcagat
     4321 aacgtagaaa ctcccgggtg cggctgccgt gaagatcgac gatggtcata tgtgacacat
     4381 caaacattcc ggcatcggta cgtaccgcat gatgttcgtc gatttgcgaa ccgtaatgca
     4441 gcggcatcat ccagccgtgg aaatccacca tgcgagcgcc gcaaagcgtg tgttgttcat
     4501 acaaaggagt ctgttgtgcc atcttgtcct cattgaataa gcggggctga caactttttc
     4561 atggtgaaat tatcaccacg aaacccagca tcggagccac tcccggtccc caacgcaatc
     4621 gttctctttt gcctgaactt accaccgaaa cagactgtta accataaggt taaattgatc
     4681 atcacattag cttatggtta aaaaacgcca aaatcgtaac agaataaaaa accaaaaaat
     4741 gaacaaatct ctatataaag ataaatagat gagaaagtta atttgaataa gacaacatta
     4801 agaactaaaa aaatgtcaaa aaacactaaa tcaaaaattc gtggcattag aaaatataat
     4861 gcgaaatcgc aggtgaaatt agtttatttc aaatgaggaa aatctcccgg cgaaaaaacc
     4921 gggagatgaa agtgtgatgg gtat
//
LOCUS       DAOTRK010000232         4883 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.234, whole genome shotgun sequence.
ACCESSION   DAOTRK010000232 DAOTRK010000000
VERSION     DAOTRK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.234"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            149..319
                     /locus_tag="QIE41_004504"
     CDS             149..319
                     /locus_tag="QIE41_004504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533287.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
     gene            complement(381..1154)
                     /gene="zupT"
                     /locus_tag="QIE41_004505"
     CDS             complement(381..1154)
                     /gene="zupT"
                     /locus_tag="QIE41_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDS3533288.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            1297..2085
                     /gene="ygiD"
                     /locus_tag="QIE41_004506"
     CDS             1297..2085
                     /gene="ygiD"
                     /locus_tag="QIE41_004506"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDS3533289.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVE
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(2131..2802)
                     /gene="dsbI"
                     /locus_tag="QIE41_004507"
     CDS             complement(2131..2802)
                     /gene="dsbI"
                     /locus_tag="QIE41_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HDS3533290.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRFLWLLMAIAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(2817..3485)
                     /locus_tag="QIE41_004508"
     CDS             complement(2817..3485)
                     /locus_tag="QIE41_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HDS3533291.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYEVAKIQGVPAYVVNGKYLIYTKSIKSIDSMADLIRE
                     LASK"
     gene            complement(3503..>4883)
                     /locus_tag="QIE41_004509"
     CDS             complement(3503..>4883)
                     /locus_tag="QIE41_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HDS3533292.1"
                     /translation="ISDLQQTKVIKVAPGFEDRLYLVNTHTFTAQGSDLHWHGEKDKN
                     AGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQNTFYDGRDRDINKRGYLMGIRE
                     TPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADATHESIETPNGTVLLRVGKSNYR
                     RDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKRDALLGALDAGAVCVNVDLAHA
                     GQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDDSIILSSRHQGVVKIGRDKQVK
                     WILAPSKGWEKPLASKLLKPVDAKGKPITCNENGLCENSDFDFTYTQHTAWISSKGTL
                     TIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQQVWEYGKERGYDFYSPITSII
                     EYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYKTKEVKVEIDVLSDKPNQTHYRAL
                     LVRPQQMFK"
BASE COUNT         1250 a         1171 c         1190 g         1272 t
ORIGIN      
        1 ccagatatgg tatataatac aacacttact atgatcgaaa tcatatactt gatccagaat
       61 tatgcgagta tatgaatgta ttcaacgatg gatatgtgaa attttaacgc taaatgttta
      121 gtaatgttaa attttgaagg tgaaaaggat gtttattgcc tggtactgga ttgtattaat
      181 tgttctactg gctggtggct attttcttca tcttaaacgt tactgtaaag cttttcgcca
      241 tgacagagat gcgctgttag ctgcccgaag taaattagat aaacgctccg ataaagaagt
      301 gtttgaaaag catcaataat acctttaata acgatacaga tgttgccttt agcaatgggc
      361 aacatctgtc attatcgtct ttaaccaatt cccgccgttt gtagcagcac taaactgaat
      421 cccatcactg acattccaca tagtacgcca tagctggggt tattattagg gtcaatctct
      481 ttggcgagcg gcattaattc atcaaccgag agcgccacca taattcctgc aaccgccgcc
      541 atgattgccg ccatgaccac cggggaaatc atgctaccga ggattaacca cgccagcaca
      601 ccaccaagaa tttctgccag tccagaaatc cccgcccaca gaatcgcggt acgtttcgat
      661 cctgttgcag cgtaaaccgg ccctgcaacg gccagacctt caggaatatt gtgcaacgct
      721 acggccaggg cgataccaaa tcccagctcc aggttgctgc tcgccgtgac aaaggtggca
      781 atcccttccg ggaagttatg cagactgata ccgagagtga gcagaatggc tgtgcgcttg
      841 atcgattttg gcaacggctg caccgatttt tgcattaaat cctgcggatg cgcatgtggc
      901 aacatgcggt ccaggccaaa atagccaagc agaccgaaga taaacattcc ataacccaac
      961 acaggcgaca ttccttcggc ggctagtgcg gcaggaagca tttccattaa tgagatgagc
     1021 aacatgatcc ccgccgcaaa tcctagcgaa aacgccagta agcggttcga gggtttttgc
     1081 ccgagaacgc cgagaaacgc gccaataaac gtggctgccc ccgccagtat ggtcagaatg
     1141 agaggtactg acatcgacaa ctccttataa atcttcacct tcaggcagat cgtcaaaata
     1201 actgatgatc atcatcattg ttatccgggt tcttactccg ccgaacaagc gtattgtgag
     1261 gatatcaaaa tgacaccttt aattaaggat atcatcatgt cttcaacacg tatgccagca
     1321 ttgtttttag gtcacggtag tccgatgaac gtgctggaag ataatttgta tacccgcagc
     1381 tggcagaagt tggggatgac attgccacgc ccgcaagcga ttgtggtggt ttcggctcac
     1441 tggtttaccc gtggaacagg agtgaccgcg atggagacgc cgcccacgat tcatgacttt
     1501 ggtggcttcc cgcaggcgct gtacgacacg cattatcctg ctccgggttc gcctgcgctg
     1561 gcacagcatc tggttgagct gttagcgccg gtcccggtgg agctggataa agaagcctgg
     1621 ggctttgacc acggttcctg gggagtgctg attaagatgt accctgacgc cgatatcccg
     1681 atggtgcagt tgagtatcga cagtagcaaa cctgccgcct ggcatttcga aatggggcgc
     1741 aaactcgcgg cgctgcgtga tgaagggata atgttggtcg ccagtggcaa cgtggtgcat
     1801 aacctgcgta cagtgaagtg gcacggtgat agttcaccgt atccgtgggc gacgtcgttt
     1861 aatgagtatg tgaaagcgaa tctgacgtgg caagggccag tggaacaaca tcctctggtg
     1921 aattatctcg accatgaagg tggcgcgtta tcgaacccaa cgccagagca ctatctgccg
     1981 ttgttgtatg tcttaggggc gtgggatggg caggagccaa ttaccattcc ggtcgatggt
     2041 atagaaatgg gcagtttaag tatgctgtcg gtgcagatag gttaataaaa gaacccgaag
     2101 ccagaacgac tggcttcggg tgttgctgag ttagcctctg atgattttaa gcgcccatgc
     2161 gccactcata atgaccagca gcaccagaca cataccaaaa gccagcatac aagcctgagc
     2221 catattcata aaatgccatg gtggtagcag ataccagcct tcagattgct gatacatttc
     2281 cacaaaccac tgttgcgtgc tgctcagtgt gacgccatca ggaacgattg gtgcgtcata
     2341 gccacaatcc ccggtaggct tgaaccaatt tggtgcccac tgtgccagcg gcaggttaaa
     2401 gggaaaagtt ggatcggtag agcaaccctg tacgccaaac agtgaatcgg gatccggatt
     2461 atgtactgca tggtggatat cgttcagttt gagcgaaaac ttcagtccca aaatgctgcc
     2521 gtaaaatgcc atcacgcagc caattagctt taggatgatg ttctttgggt tgatcgctgc
     2581 aaccaatcct ccaataacca tcacaaacat ggcgtagcga atgtatacac attgctcaca
     2641 gggtgccatg tagagataga tctggaagaa agagtgcgcc aggataatta acgcgcccat
     2701 cgcaatggcc attaacaacc acaaaaaacg ttgttcttgc catcttacca gcgtgtccac
     2761 cggtgaagtg cgaaggtctt tccacattcc cttaataccc atagcctgtt tccctgttat
     2821 ttgcttgcta actcgcggat aaggtctgcc attgagtcaa tagatttaat actcttggtg
     2881 tagatcaggt atttaccatt gacgacatac gccggaacgc cctggatttt tgcaacttca
     2941 taagaggctt tccatttttc cagcgtttcc tgaactgcag gctctttcag cgcggcttca
     3001 aaatcggcct gactcatacc tgcggcgtcc agtccggttt tgataaaggc agccggatct
     3061 ttaccatcag accagcgttc ttttttgtca tgataagccg cgtaataagc aaacttggct
     3121 ttcttaaact gagagttagc atcaaataat gaaatacctg ctgctttatc tttattgatc
     3181 aagaccgcaa agacttcgct tgcttgtttg ccgtattcac cttttgtttc cagatggaat
     3241 ggagtaaaag caacgatatc tttcactttt tccgataccg gaccggtaac cgctttgtcg
     3301 tacttgtaac agaacgggca ggcgtagcta aataccttaa tcagcgtttt gtcggcattc
     3361 ggaataggtt tttccagaac catgtaatcc gtgccttcgg taaatgcaga agcggtaaat
     3421 gaaacagcca aagctgcggt aagcagtatg gttttaaata gtgatgagat ccctaatttc
     3481 gacattttta ttcccttctt aattatttga acatctgttg tggacggact aacagtgcac
     3541 gatagtgagt ctgattgggt ttatctgaca gcacgtcgat ttccactttc acttctttgg
     3601 ttttgtaatc gatttcgttc aacttaccga cggttggctg cccgacatcg aacaaatgaa
     3661 tagaaccacc gaagccaaac atggtgttac ggtcggcttg atattcaatg atggaggtaa
     3721 tcgggctata gaaatcgtag ccacgttctt taccgtattc ccacacttgc tgaacggtgc
     3781 ctttcttctc atcaatctta tattccacaa agcgggagta tttcatggtt ggtaaggcag
     3841 gttgttccag atgacgccca tcgccattat caaaaatggt gagcgttcct ttgctggaaa
     3901 tccaggcggt atgctgggtg taggtaaagt cgaagtctga gttttcgcac aggccatttt
     3961 cgttacaggt aattggctta cctttagcat caaccggttt cagcagcttg ctggccagcg
     4021 gtttttccca acctttagag ggcgcaagga tccatttcac ttgcttatca cgaccaattt
     4081 tcacaacacc ctggtggcga gaggagagga tgattgagtc atcttttgcg tcataggcga
     4141 ttgagttaac gtgcgcccag ttacgacctg gccctacacc cagcgcgtcg ccgaacggtg
     4201 tatctggttc cagttttgcc tgttgtcctg catgggcaag gtcaacgtta acgcaaactg
     4261 cacctgcatc cagcgcgccg agcagtgcat cgcgtttcgg atcgaggatc ttggtcagat
     4321 cccatacatc gacaacgcga ccagatttat cgacttcgag gatatggtca cgaatggtgg
     4381 tgacgtgtac gccgtcatcg cgacggtaat tgctcttacc tacgcgcaac aatactgtgc
     4441 cattaggcgt ctcaatggac tcatgagtgg cgtcagcaaa tccgcgcggt agtttgtgat
     4501 cttcgagcac ctgccccatc atgtcgaact cgtaccagtg ctgaccttgt acagcggtaa
     4561 aggtgccgcg tggtgtttcg cggataccca tcagataacc acgcttgttg atatcgcggt
     4621 cacgaccatc gtagaaggtg ttttgatcca accaccagcg gtattcgcct tccgtatcga
     4681 cgataaaggt gaatggcgcg atatcaaaag ggagtgcgcc agttgccgga cccgcatcaa
     4741 ggataccggc atttttatct ttctcaccat gccagtggag atcggaacct tgggcggtaa
     4801 aggtgtgggt attaaccaga tagaggcgat cttcaaaacc cggtgcgact ttaataactt
     4861 tggtctgttg taaatcggag ata
//
LOCUS       DAOTRK010000233         4812 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.235, whole genome shotgun sequence.
ACCESSION   DAOTRK010000233 DAOTRK010000000
VERSION     DAOTRK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.235"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..593)
                     /locus_tag="QIE41_004510"
     CDS             complement(<1..593)
                     /locus_tag="QIE41_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDS3533293.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="QIE41_004511"
     tRNA            complement(708..783)
                     /locus_tag="QIE41_004511"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="QIE41_004512"
     tRNA            complement(790..864)
                     /locus_tag="QIE41_004512"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="QIE41_004513"
     tRNA            complement(981..1065)
                     /locus_tag="QIE41_004513"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="QIE41_004514"
     tRNA            complement(1074..1149)
                     /locus_tag="QIE41_004514"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="QIE41_004515"
                     /gene_synonym="panK"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="QIE41_004515"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDS3533294.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAINTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3455)
                     /gene="birA"
                     /locus_tag="QIE41_004516"
     CDS             complement(2493..3455)
                     /gene="birA"
                     /locus_tag="QIE41_004516"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDS3533295.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAE"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="QIE41_004517"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="QIE41_004517"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDS3533296.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLKDYRGTVIINRIKGIEIHDELDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4617..4732)
                     /gene="rrf"
                     /locus_tag="QIE41_004518"
     rRNA            complement(4617..4732)
                     /gene="rrf"
                     /locus_tag="QIE41_004518"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1206 a         1223 c         1107 g         1276 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgggtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaatgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgcgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacagtgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgccag
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgctgtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcacgactga tggcttcctt caccctaatc aggtactgaa
     1921 agaacgtggt ttgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcacctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaacggtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt tcctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagcg attaatactg ccatgacgtt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgcgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcggtagaa gaggtcaggc tacgcaaata
     2461 attttcaggg gagctaatgc tcccctatct tattattccg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagca ataaagcgcc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccagcttttc ccagcgcgaa agataaggtg ccaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatg agcatggccg ccaacgtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccttgctcca gacgccagaa catcgacaaa
     3061 tataagtttg cgccaaaagg cgaaaaccat ttccgccccc ggcgaccacg gccagcctgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atctgttcag cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcaccgtt cgctaatagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatggtgt gccagttgca ccacatcttc gctttttgca ttgtcttcat
     3601 taatgagaac taacgcctgt tgacggtgca ccgcagcccc acccatttgc atccctttta
     3661 gctggcactg atcgataagc caacctgctg ccagttttac tgaaccatcc gcctgggggt
     3721 aatttggtgc tgttggaaat tgtgacagta atgctttagc cgtttcggca gatacaacag
     3781 ggtttttgaa gaaactaccg gcattgccat tcacttttgg gtcaggaagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac tgttgtagga tccagacgag
     3901 ttaagtcacc atacgttagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcggtcctgg tattcatgtt taaaaatact gtcgcgatag ccaaaacggc
     4021 actctttggc agttaagcgc acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 cacaaccagg aattaacgcc agattttcca gacctggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaagttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt gccgcgatag tctttcagaa
     4321 aaagtacatt acttccttca cccagaataa gaacgggttg cccttctgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tccgtggggg gatggcttgt agtaatgacg acagggagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc ttctgcttaa tttgatgcct
     4621 ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
     4681 cttctgagtt cggcatgggg tcaggtggga ccaccgcgct aaggccgcca ggcaaattct
     4741 gtttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa tcttctctca
     4801 tccgccaaaa ca
//
LOCUS       DAOTRK010000234         4809 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.236, whole genome shotgun sequence.
ACCESSION   DAOTRK010000234 DAOTRK010000000
VERSION     DAOTRK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.236"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..464
                     /gene="espX1"
                     /locus_tag="QIE41_004519"
     CDS             <1..464
                     /gene="espX1"
                     /locus_tag="QIE41_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HDS3533297.1"
                     /translation="SYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSNIFGTS
                     FTNTKIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLIQT
                     LVRDNVKLNNDVYKELNKFMHDYDKTSPEMRKYLQSINECMLLMKNIVHQD"
     gene            766..1299
                     /locus_tag="QIE41_004520"
     CDS             766..1299
                     /locus_tag="QIE41_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3533298.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLSAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            1349..2032
                     /locus_tag="QIE41_004521"
     CDS             1349..2032
                     /locus_tag="QIE41_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDS3533299.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            2045..4494
                     /locus_tag="QIE41_004522"
                     /pseudo
     CDS             2045..4494
                     /locus_tag="QIE41_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            4507..>4809
                     /locus_tag="QIE41_004523"
     CDS             4507..>4809
                     /locus_tag="QIE41_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDS3533300.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCAVTNVGEQRGTYILKPLSMKGNL
                     TTEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIE"
BASE COUNT         1601 a          939 c          921 g         1348 t
ORIGIN      
        1 tttcatatgg gtattatttt ggttgtgtat tatccaatat atcatgtttt gaaagcgact
       61 tatcaaacac catcttttca aatggagaaa taaataacct cttcattaaa aaaagcaaca
      121 tatttggaac atcgttcact aataccaaga taaaaaattt gcgctgtgaa gatattatgc
      181 ctggtcgatg gaccactcaa cttgtcaata agcacttagg ttacagatat acaggagtat
      241 tcaaaaccct ggcaagtatt gatgacaaac cgtcccgttt cgaaattttg ataccattga
      301 tacaaacttt agttcgcgac aatgtaaagc taaataatga tgtgtataaa gaacttaata
      361 agtttatgca cgattacgac aaaacatccc cagaaatgag aaaatacctt cagagcatca
      421 acgagtgtat gctccttatg aaaaacatag ttcaccaaga ttaaaatact caaagaaaga
      481 agtaatttca gaatgaaatt gtctgtgatg attattaata cactgcagag aaataaaaaa
      541 caatctgaag catgctctga aacatcctac tctgtcactc taatgatggc accttgctct
      601 tcttataaag aagggaacaa ttaagaatat taaattttca aagtttcatc aacttcacat
      661 caagaaatta aaaaatttaa acattaatct aaataataac aacctgatca cataatcggg
      721 tttgaaaacg agtgaccttc cataccagtt aattaaggat gatttatgaa aaagtatatt
      781 attcccttcg ttgcgacaac gttgatgttt agcgcaacaa ccttcgccaa ggatgtcgat
      841 caaggaacgc taaccattag aggagaagtt gttgatacaa cgtgttactt tgttaatggt
      901 gattcgacaa cagatatcgt tgtcgaagat gttgtaacca gtgcaatgaa tcaacttatc
      961 aataatgaaa tctatacttt aaaaaccggt tcaacctctc atgacttaga aattcagtgt
     1021 gacggcaata cagccccaag tatcgaagtg ctagcatcag agtttgatgc taataacttt
     1081 acgctgaata aaggaactgc tgagaacatt ggctttgcca ttttcctcga tgatggaact
     1141 ttgaccagaa tgagaccggg gttaaaaaca ccgctgaaag ataatggctc tggtcaatat
     1201 tttcttaatt taagtgcaca atatgcccgc acaagtggca atcctgttac aaaaggctcc
     1261 gtagagtcaa ccgtaacgat taaaatcagt gcagattaac gttttttgcc ggtagctctg
     1321 ctaccggtat tatttacgga gtgattacgt gagaataata acagcattac tgatgtcatt
     1381 ttttatttta cctgttcatg ccggtgtggt catctatggt acgcgcatta tttatccgga
     1441 aaaaaacaga gaagttctcg tacagttaat gaatcaaagt aaaaatgcct ctttgattca
     1501 ggcatggatc gatgatggga acaccactat cgcgccagaa aaaattcagg tcccatttat
     1561 tctcacaccg ccagtaagtc gtgtagcagg tggttctggc caacaactta aaataagaaa
     1621 aatgccaaac aatttgcctc acaataaaga aagtttattt tatctaaatg ttctggatat
     1681 ccctccaaac aacccacaaa atgcaggaaa aaacaaaatt aaacttgcat tgcaaaaccg
     1741 tataaagtta ctttggcgtc caagcggaat cgctccggta gataaaaaaa gcctttctca
     1801 attaaatatc aaaaagaaaa ataacgccat ctcaataaac aacgaaacag ccaactggat
     1861 aacggttaca actatcaaag cacaaaacgt gaaagttaat aatgaaagta tcttactgcc
     1921 tccattttcg aataacgaca ttacattaaa aaataaccac gcatcagaat atgaactaac
     1981 ggtagttgat gattacggga ataacatcca tagtaaaatc gccgcgagat aacaggaaaa
     2041 agtcatgtta aggacgacac gatgggtcgc tgctattatt tttttgtatt cttttccggg
     2101 ttatgctgaa gaaacttttg atacgcactt catgattggc ggcatgagag gagaaaaagt
     2161 ttccgaatat cgtttcgata ataaacaacc gcttccagga aactacgaac ttgattttta
     2221 tgtaaatcac cagtggcgtg ggaaaaaaga tatcacgatc ccagagtcac cagccaaacc
     2281 atgtctgcct aaggtgctgc taaccacact gggggtaaag accgataacc tcaatacaga
     2341 agataattgt attttactgg atgaagcggt tcatggtgga caatatcagt gggatatcag
     2401 tgaacaccga ctaaatttaa cagtaccaca ggcgtatatc aatgaacttg aaagaggcta
     2461 cgttccccca gaaagttggg atcgcggcat tgatgctttt tatacctcct ataatttaag
     2521 tcagtatcgc tcttatgaca gcaataataa cagtaataca gccagttatg gccggtttaa
     2581 cagcggatta aatttattta gttggcagtt acactctgat gccagttata gcaaacctga
     2641 tgatatgaaa ggcaaatggc aaagtaatac tctttacctg gaacgtggct ggtcgcaaat
     2701 attaagcaca gttcagatag gagaaaatta cacatcttca ctcatattcg actctctgcg
     2761 atttagcgga atacgcttat ttcgcgatat gcaaatgcta cctgattcta tgcaaagctt
     2821 tacaccttta gtacaaggtg ttgcgcaaag taacgcctga taacggtttc gcaaaatggc
     2881 tataccattt accaaaaaga ggttccccca ggaccattta ctatcgcaga tttgcaactt
     2941 tcgggcagcg gctctgatct cgatgtcagt attaaggaag cagatggtag tgttcgttcc
     3001 tttttggtgc cctactcttc cgtgcccaat atgctgcaac ctggtgtttc aaatttcgat
     3061 tttaccgccg gacgcagtca gatatatggt gtcaagaatc aggaggactt tctcgaggca
     3121 aattatatat atggcctcaa caatcttttg acactttatg gcggtacgat tttatccgat
     3181 aactataatg ctataactct gggaaatggt tggaatacac cgctgggagc aatctctttt
     3241 gatgctaccc gatcgagtag taagttaaat aatgatacca gacacgaagg tacgagctat
     3301 caggtcgcct ataataaata tctattgcaa acggctactc actttagcgt ggccgcctgg
     3361 cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa tgataaaata
     3421 aatcatcaga gtgactatga cgatttttat gatattggaa gaaaaaatag cctttctgcc
     3481 aatatcatgc aacctttatc caataatctg ggaaatgtat cattaagtgc gctttggcgg
     3541 aattactggg ggcgaagtgg aaatgctaaa gattaccaat tcagttattc caatagctgg
     3601 caacgcatca gttatacttt ctctgccagc caatcctatg atgaaaacga caaagaagag
     3661 gagcgtttta atctgtttat ctccattcct ttctactggg gggatgatat tgccaaaaca
     3721 cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta ttcctccaac
     3781 aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg tatatatgtt
     3841 aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg gagaactccc
     3901 atcgccacaa tagatggcag ctatagccat tctaaaaacg cctggcaaag tggtggaagt
     3961 attagtagtg gattagttgt ctggcccggt ggtattaata taactaacca gttatccgat
     4021 acatttgcaa ttctggatgc gcctgggtta gaaggcgcgc atattaatgg acaaaaatac
     4081 aaccgaacaa acagcaaagg ccaggttgtt tacgacctga ttatacctca tcgtgaaaac
     4141 catctggtgc ttgatatagc aaacagtgaa agtgaaacag aattgcaggg caatcgtcaa
     4201 attattgcgc cttaccgtgg agcagtttct tatgtgcagt ttacaactga ccaacgtaag
     4261 ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg ctatgacgta
     4321 ctggatctcc aggaaaacaa tattggagtc gttggccagg gtagtcgcct ttttattcgc
     4381 gtagatgaaa ttcccactgg cataaaagtt gctctcaatg atgaacagaa cttattctgt
     4441 actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca gtaaaaggag
     4501 tttactatga agtggctttt attaatcacc ttgagtctat attcttttat tgtgcaaagt
     4561 gctccctgtg cagtgaccaa tgtcggtgaa caacgtggaa catatatact caagccactg
     4621 agtatgaaag gtaatctaac caccgaaaaa attgaaaaca ccgggaatat cattgagtgt
     4681 gcagacttgc aagagacgac caacgccatt aaagttcatt ttttaacaca gaacgcttta
     4741 aggtttaaag tcggaagtcg tcattacatt attaatttcc cctttgaatc tgacggtaaa
     4801 aaaatagag
//
LOCUS       DAOTRK010000235         4798 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.237, whole genome shotgun sequence.
ACCESSION   DAOTRK010000235 DAOTRK010000000
VERSION     DAOTRK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.237"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            250..1224
                     /gene="hemB"
                     /locus_tag="QIE41_004524"
     CDS             250..1224
                     /gene="hemB"
                     /locus_tag="QIE41_004524"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDS3533301.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1264..2115)
                     /gene="tauD"
                     /locus_tag="QIE41_004525"
     CDS             complement(1264..2115)
                     /gene="tauD"
                     /locus_tag="QIE41_004525"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDS3533302.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRAQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWSSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2112..2939)
                     /gene="tauC"
                     /locus_tag="QIE41_004526"
     CDS             complement(2112..2939)
                     /gene="tauC"
                     /locus_tag="QIE41_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDS3533303.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAILLTVWWAVAAL
                     HLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2936..3703)
                     /gene="tauB"
                     /locus_tag="QIE41_004527"
     CDS             complement(2936..3703)
                     /gene="tauB"
                     /locus_tag="QIE41_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDS3533304.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESCELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVAFGLQLAGVEK
                     VQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFITMREYVLSRVFEQREAFS"
     gene            complement(3716..4678)
                     /gene="tauA"
                     /locus_tag="QIE41_004528"
     CDS             complement(3716..4678)
                     /gene="tauA"
                     /locus_tag="QIE41_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS3533305.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1110 a         1383 c         1224 g         1081 t
ORIGIN      
        1 ataagcaatg aactttaagt tattcacttt ttgaaaatgt caccaaatcc ccaaaaacaa
       61 taatctatgg atataaataa aaattaaaaa tattttcagt gaatataaaa ttattgctat
      121 ttctattaca aagatgtcct ggcctctctt ccattctctt cttgtcaaca tcgcgacaac
      181 tttcgtaaaa catccccacc ctgcttcagg tatactatgc ccctcgattc cacaaacatc
      241 aggcagacca tgacagactt aatccaacgc cctcgtcgcc tgcgcaaatc tcctgcgctg
      301 cgcgctatgt ttgaagagac aacacttagc cttaacgacc tggtgttgcc gatctttgtt
      361 gaagaagaaa ttgacgacta caaagccgtt gaagccatgc caggcgtgat gcgcattcca
      421 gagaaacatc tggcacgcga aattgaacgc atcgccaacg ccggtattcg ttccgtgatg
      481 actttcggca tctctcacca taccgatgaa accggcagcg acgcctggcg ggaagatgga
      541 ctggtggcgc gtatgtcgcg catctgcaag cagactgtgc cagaaatgat cgtcatgtca
      601 gacacttgtt tctgtgaata cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc
      661 gtcgacaacg acgcgactct ggaaaattta ggcaagcaag ccgtggttgc agctgctgca
      721 ggtgcagact tcatcgcccc ttccgccgcg atggacggcc aggtacaggc aattcgccag
      781 gcgctggacg ctgcgggctt taaagacacg gcgattatgt cgtattcgac caagttcgcc
      841 tcctcctttt atggcccgtt ccgtgaagct gccggcagcg cattaaaagg cgaccgcaaa
      901 agctatcaga tgaacccaat gaaccgtcgt gaggcgattc gtgagtcact gctggatgaa
      961 gcccagggcg cagactgcct gatggttaaa cctgctggag cgtacctcga catcgtgcgt
     1021 gagctgcgtg aacgtactga attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg
     1081 atgattaagt tcgccgcgct ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc
     1141 ttaggttcga ttaagcgtgc gggtgcggat ctgattttca gctactttgc actggatttg
     1201 gctgagaaga agattctgcg ttaatgttca ttatgccggg ttatccaacc cggcatttcc
     1261 cccttacccc gcccgataaa acggtttatc cccaaggatc gtcgcccgat gcataatccg
     1321 ccgctgtggc aggtaatccg cattggcata gtgctgagtc acgcgattat cccaaatcgc
     1381 aatatcattc ggttgccagc gccagcgcac ctgaaactcc ggtttggtga tatgggcaaa
     1441 caaaaaactt aacaaggctt cgctctcttt ctcgctgaca tcaacaattc gcgtagtaaa
     1501 gccttcattc acaaacagcg cctgtttacc gctcaccgga tgcgttcgca ccaccggatg
     1561 tagcaacggc gggtttttcg cgaccgcctc gcgccagcgt tgatgctcct cctcggtttt
     1621 gcggtatttg tattccggga acgatttacg gaaatcatgc tccgcacgca gcccgctcag
     1681 cagttggcgg aagggaacag agagcgcctc ataggccgca ataccgctgc tccagagcgt
     1741 atcgccgccg gtcgagggta actcttttgc cgccagaatc gcccctgcgg gtggcgtttc
     1801 aataaatgtc acatcggtat gccagttgtc gttatccggc ggattatcgt tatgagtatc
     1861 cagcacgatg atctcgtcaa ccccttcggc atgagggtaa acagggtgaa tatgcagttc
     1921 gccaaaacgc tgggccagcg cgcgttgctg ctgcggcgta atagcttgag cgcgcagaaa
     1981 caccacctga tggcgcagca ccgcatggta aagctgttca aactgattat cgcttaacgg
     2041 gcgcgtcagg tcggcacccg aaatttgtgc gccaatatac ggccccagcg gggtaatgct
     2101 cagacgttca ctcattgtac ttctccatgc cagggcgtca ggcggcgctg taacgcgcgc
     2161 aaacccagtt ctaaaagaaa agcgataatc gcaatcaccg cgatccccgc cagcaccacg
     2221 tcagtcgcga gaaattcacc cgctgactga accataaatc ctaaaccgcg cgtcgcggca
     2281 atcagctccg ccgctaccag cgtagaccag cccacaccca gaccaatgcg taatccggtg
     2341 aggatttccg gcagcgcgcc gggcaaaatg acaaaccaca gcacctgcgc acgactggca
     2401 cccagcgact gggcggcacg aatgcgaacc tgctgcgcgc ttttcacccc cgccagcgcc
     2461 gacatcgcca ccggtgcaaa aatcgctaaa tagatcagta agatcttcga ggtttcacca
     2521 ataccaaacc agatcaccat cagcggcaaa tacgccagcg gcggcaccgg acgataaagc
     2581 tcgattatcg gatccagaat gccgcgcacc gtcgggctta gtcccatggc gatcccgacc
     2641 ggaatgccga tcaacaccgc cgcaagtagc gccagcacaa tgcgcgtcag gctggctgca
     2701 agatgctgcc acagggtggc gtccataaat ccctgcggcc cggcaatggt gagcagtttt
     2761 gccagtacct gttgcggtgg cggcaaaaac agcgggctaa tcaggtgcag cgccgccact
     2821 gcccaccata cagtgagtaa aatcgctaac gtgccaatgc ttaaggtcac ctgacgcgag
     2881 agcggccagc gccatttcag ccgatgcgaa tgcagttttt cattaatgag cacactcatg
     2941 agaacgcctc ccgttgctca aatacgcggc ttaaaacata ttcgcgcatg gtgatgaatt
     3001 gtggatcgga cttgatgctg cggctcgact ctcccgcaac aaagcggcga gcaaagttga
     3061 gcggcagccg ctccagcacg cggccagggc cgggtgaaag cagaaccagt tcagtcgcca
     3121 taaacaccgc ttcttctata tcgtgggtaa tcaacagcac ctgcttgccc gtctcctgcc
     3181 agagtttcag cagcagagtt tgcatctgat cgcgggtgaa ggcgtccagc gcgccaaacg
     3241 gttcgtcgag taataacagc tggggattcg ccgccagcgc gcgggcaatc cctacccgct
     3301 gacgttgacc gccggaaagc tgccagatat agcgtttttc tgcgccttcc agccccactt
     3361 ttttcagcat ctggtgcgcg atttccaggc gctgtacttt ctctacacct gccagttgca
     3421 ggccgaacgc cacgttgtct accacattac gccacggcaa cagcccttca ttctgaaaaa
     3481 ccacgccccg ctctgcgccc ggtccctcaa tgcacttacc cgccagttga atgctgccat
     3541 gctgataagg cacaaaaccg gcaatcagat tcagcaaggt ggttttgccg cagccggacg
     3601 gccccagcac caccagtagc tcacagcttt ccagcgtcag gttgatatct tccagtgccg
     3661 gtttgccgcc ataatcggcg taaagatgag agatttgcag catctgcgcc tccttttatt
     3721 gcacgaagcg cgaggtcacg tactggctgt aatcattcgc tacagccggg actttgccct
     3781 gctctttcaa aaactgcgcg gtgtcgatga tcgctttgtt caccggtccg gtcagttctg
     3841 ccgtttgctg ctgcggcgtc agataggtat tccccttcac cagccccgga acgtcacctt
     3901 caggtacgcc gcttaaacgt gccagtttgc tgatgttttc cggctgtttc agccacgcgt
     3961 ctggattagc aatgtacggt tgttgagcat cgatggcgct tttagcgaac gctttcacga
     4021 cctcaggatg tttctcggca aaatctttgc gcactaccca gacatccagc gtcggcgcgc
     4081 cccactgccc gacctgttca gaatcggtca gcaccttgcc gtctttttcc agggcgttaa
     4141 ccgccggtgc ccagacataa gcaccatcaa tgtctccccg ttgccatgcg gcgataatcg
     4201 cgggtggctg caggttcact atctccactt gtccgggttt tataccccag tgtttcagcg
     4261 ccgccagcag gctgtagtgg gtggtggaga taaacggtac ggcgatgcgt ttgccaatga
     4321 gatcttccgg tttgctgata gttttcttta ccaccagcgc ttcggagtta cccagttttg
     4381 acgccagtaa gaagacttca atcggcacct gttggctggc tgccaccgct aatgggctgg
     4441 aaccgaggtt accgatttgg acatcgcctg aagccagcgc ccgcacgatg ctggctccgc
     4501 tgtcaaactt acgccagtcc accgtcgccc cgctctcttt agcaaaggtg ttatcagcct
     4561 gagccacttt cgccggttcg gcggaggttt gatacgccac ggtgacgttc accgcctgtg
     4621 cctgaaaagc gatgaatgcc agtgcggcaa gaagtgtgtt acgcgatgaa attgccatga
     4681 ttgtctgctc ccctgtcttg ttataggagc agtattcagg aataaaaaca ttcattaaaa
     4741 gaattagtcg ttatcgcaca gatgatttta ttcttagcaa aaaagcggca aaggaaag
//
LOCUS       DAOTRK010000236         4778 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.238, whole genome shotgun sequence.
ACCESSION   DAOTRK010000236 DAOTRK010000000
VERSION     DAOTRK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.238"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..966)
                     /locus_tag="QIE41_004529"
     CDS             complement(<1..966)
                     /locus_tag="QIE41_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDS3533306.1"
                     /translation="MMFRNGMLLIFILWVNFTWAGCRTTASLNITDSINVGEILANEV
                     SFSKSVVFTGISCDTSTDKIVYKNIQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKS
                     SNAQAVLPYVVKISKGTPDFTGERKPTWFLSDTVIANIGGESSSSIDFWLSICKTLKI
                     NWCVSYLTSKLAGDTFTLGLSISYYPKNTTCKPENTIIKVDDIALSQLRSQGKITANS
                     KEGIITLKCDNLFGDTKQASRNMVVYLSSSDLVTGSNTILRGNIDNGVGFVLDLKEPP
                     KGTEAAIKISASGDQGAATSLWKTDKTGASLNSNIINIPVLASYYV"
     gene            complement(977..3457)
                     /locus_tag="QIE41_004530"
     CDS             complement(977..3457)
                     /locus_tag="QIE41_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDS3533307.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTSW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSKSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICR"
     gene            complement(3473..4147)
                     /locus_tag="QIE41_004531"
     CDS             complement(3473..4147)
                     /locus_tag="QIE41_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDS3533308.1"
                     /translation="MKGLFALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(4228..4770)
                     /locus_tag="QIE41_004532"
     CDS             complement(4228..4770)
                     /locus_tag="QIE41_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDS3533309.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1330 a         1165 c          875 g         1408 t
ORIGIN      
        1 gacgtagtaa ctggccagga caggtatatt aataatatta ctatttaaag aagccccggt
       61 tttatctgtt ttccataatg atgtcgccgc gccctgatcg ccgctagcag aaattttgat
      121 ggctgcttca gtccctttgg gtggttcttt tagatccaac acaaatccta caccattatc
      181 tatgttgccg cgcaaaattg tattgcttcc tgtaactaag tcactgctgg aaagatatac
      241 gaccatattc cgcgatgctt gttttgtgtc gccgaaaaga ttatcacatt tcaacgtaat
      301 tattccttcc ttactattcg ccgtaatctt tccctgactt ctgagttggg acaaggcaat
      361 atcatctact tttattattg tgttttcagg cttacaggtc gtatttttag gataatagga
      421 aatacttaat ccaagcgtaa atgtatcccc cgccagttta ctggtgagat aacttacaca
      481 ccagttaatc ttcaatgtct tacaaatgct caaccaaaag tcaatggatg aagatgactc
      541 accgccaata tttgcaatca cagtatctga aagaaaccag gtaggttttc tttctccagt
      601 aaaatcaggt gtgcctttag atattttaac cacatagggt aatactgcct gcgcattgct
      661 ggatttccca atcgtgtcac tggttttacc taaagactct attttaacct ttaacttttc
      721 gccattacca aaaggcccga cttcaaccca atcactttgg atatttttat agactatttt
      781 atccgtgctc gtatcacaag atatcccggt aaacacaaca cttttactaa aggaaacctc
      841 attcgctaaa atctccccca cattaatact atcagtaata tttaatgatg cagtggtacg
      901 acatccagcc caggtaaaat taacccataa aataaatatt agtaacattc catttctgaa
      961 catcatattc cacctctcac cgacagatat aatttctact ttcatcaatc tctttaccga
     1021 aggtgattgt gcatgaaagt ccttgttgct tatcaattgc tacattaacc gatggcggta
     1081 cttcattggt gcgaataaat aactgacttc cctggccgac aacgccaata ttatgaccgt
     1141 ggatatcatt gacttcataa ccaaacgtta atggttgccc atccgctctt aacgctttta
     1201 taaaccaggg cttgcgctga tcggtatcaa aattaaccag tacaaccgcg ccgcgaaaag
     1261 gggcggcaat tttccggtta ccgcgcaatt ctgcttcgct atcgctttgc gacacatcca
     1321 gcatcaggtg attttcccga taaggtgtca ttccgtcgta taccaccact ccattacggt
     1381 ttgttgtgcg atatttttgc ccattgacat aagcatcttt aattcctggc gcattcatca
     1441 cagcaaacgt ttcggaaaga cggttcgcca gattaacgcc acccgaccag gcgacaatgc
     1501 cccctgaaac actggctcca gcctgtcgat aagtactcga cttactataa ctgccattca
     1561 ctgtcgcaac cggcgcgttc caggttaaat tcgctccagc tgtcgtttca tttccctgat
     1621 gctgatgact caggttgacc ccatagttaa attgatcccg gtttccaacg gttcccgata
     1681 atcccgtatt atttgaggca aaaccctgat catcaaacgt cgttgagtta gacatatata
     1741 tttgccgacg aggcgtcgta acgtcatcac cccaatcaaa gggaatcgat ataaaaatat
     1801 taaaacgttt ctcttcatga tgattctcgt cataagcctg gcttgccgcg agggtatagc
     1861 ttatccgtcg caggttgttg gaataactca actgataatc cttactgctg ccgctacgcc
     1921 cccagtaatc tcgccataac gtacttaagg acacagaacc ccagccttct ggcaatgact
     1981 ggctcatatt ggcagaaaag ctatttttac ggccaaaatc gttctggtaa taatcggcaa
     2041 tgtcatagat atcgttttca tcacggcgat aattatcttt attgtttgcc caaacgtgat
     2101 cgttaaatgt ccggtaatca cgcgacgaat aacgccaggc cgccagacca aaacgcgtcg
     2161 acgtttggct cacaaatttg ttgtaggcaa tttgataact ttgcccgtca aacacatcgc
     2221 cgttgtcttg tttactatgc gacttcgtgg catcgacgga aatggcacca atgcgtgtat
     2281 tccagctagt ccccaaagtg aacgcgtaat aattattcgc gaccatcgat ccgccataca
     2341 gcgtcaataa gttattaaaa ccatactgat aacccgcctg gacaaaatca ctttgtttgc
     2401 tcgccccttc aatatggcta cgacccgccg caaaatcata tttcgacacg ccaggttgca
     2461 gcatattcgg caccgctgca taaggcacca gataggtggt taccgaaccg tccgcctctt
     2521 tcacgctgac atcaagatct gctccaccac cggccaactg caaatctgta atcgcaaacg
     2581 ggccaggagg aacctctttc tgataaacca caaaaccgtt ctgttcaata gttaccagcg
     2641 cgttactctg agcaatcccc tgcacccgtg gagtaaaatt ctgtttcgag ttaggcaaca
     2701 tctgcatatc acgaaacaac cgcacaccgc tgaagcgaac agaatcaaaa atatcgcttg
     2761 atgtgtacat atcacccacg cgaagcgtgc cgagaagttg ggcaaatcca cgttccagat
     2821 acagggtatt gcttttccac acccctggat tgctatttgt tttactgaaa ctggcatcag
     2881 aatgcagttg ccaccccagt aagtttaacc cgctgttaaa acgtacatat gtactcttgc
     2941 tattacccga cgctttatag tcgctgtaat actgactcac ataataagag gtataaaacg
     3001 cattaatacc ccgctcccag ttttccggtg gaacatagcc actttccagt tcttccaccc
     3061 acgcctgcgg gacactgaaa tcgagacgaa aaactccgat atcccaggta tagctcccac
     3121 cctgaacaag ttgctcaaat gttaaacatt gcttaccgct ggcaaagtta tcggtattaa
     3181 tgcctaaccg cttgataatt tcccttgata aacatgtttc ttgcgggttg tctttaacaa
     3241 tgatctcata tttcccgcgc cattgcttat tgacataaat atcgatgtca tactgccccg
     3301 gcaagggttg attatcatca agacgaatat ttgcaacctg ctggtctttc attccaccta
     3361 tcataaaatg ggtatcaaag gtttcttcag ctgcataagc ttcaatgccg agcaataacg
     3421 ctacgattgc tgatgcaagt ggggtcattc tcaacatagg cagctcctgc aattaaattt
     3481 tgtcactaat atagttgcca tggtcatcga taatggtcag ataccagtta ttcgcattat
     3541 tacttttgac attaatactc tgactttcta agggggcaat cataatagtt tcataattga
     3601 ctttgacatt attagctttg acatccgaaa tcgttaccca atttgctgag tcatttttta
     3661 tcaccaatcc attgccactg cgatttacca gtaacttttt aaatgtcgct ttatttaccg
     3721 gtgcaatacc cgcaggccgg taaaacaatt tgattctgtt ttgcattgca aatttcagtg
     3781 cattcttgcc ctcttgctct ggactatttg gtggaatatc cagaacattc agataaaaaa
     3841 tgctctcttt attagtgggc agtttattcg gcataatttt gatttttact tgctgcccgg
     3901 aatttgcccc tatttttgcc actggtggcg ttaacatgaa aggaacctga attttttctg
     3961 gtggtaacga cgtatcacca tcatcaatcc acgcctgcag cagcgaagaa cggtttccct
     4021 gattcatcaa ctgcaccatc acttctttat tttctgccgg gtaaataata cgcgtcccgt
     4081 agataacaat accggcatgt gcaggaagga ccatagaaga aatgagtaaa gcaaataatc
     4141 ctttcatctt aatgcctctt aaattaaaag gccgccatgg ggatagcggc catgtttatt
     4201 acctggagaa acttgaaatt ttatttatta atcctgcgcc acacgcataa ccagagaaga
     4261 taacacatca cctgattgca ctgaattatc tgtcagcttc aggtattttg ctttaaacgg
     4321 aatagcatat tgaccttccg ctgttttata actctcaatg ttaaaaggtg cagaaacatc
     4381 caggacatcg ctgttatcac ctaaatagag atagtacccc acggtatcgt tgctactggc
     4441 tatcgcctta tcgtttccgg ttattttatc gttggtaatc aacgagagtt ttacaccagc
     4501 aacttctttc ggacagctaa tttttaacat gctgctggtt acaccgactg catcatcata
     4561 ggtcgagtat ttagctttac cttcaaagac agtggttggt acagttggca tattaatact
     4621 tactgaatcg ccacccgctt cgaatttaca cggagattct gcaatattcc ctttaatggt
     4681 taatgttccg gtatcaacgt ctgcggcaaa taccccagcg ctcataaaaa aagaagaaaa
     4741 gacagcggca gcaataattg aacgtttcat aatatgtt
//
LOCUS       DAOTRK010000237         4723 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.239, whole genome shotgun sequence.
ACCESSION   DAOTRK010000237 DAOTRK010000000
VERSION     DAOTRK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.239"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(39..893)
                     /locus_tag="QIE41_004533"
     CDS             complement(39..893)
                     /locus_tag="QIE41_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDS3533310.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSICRILAVRKSTLLQIDTLIRQ
                     LADISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERNEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSACSRV"
     gene            complement(978..1175)
                     /locus_tag="QIE41_004534"
     CDS             complement(978..1175)
                     /locus_tag="QIE41_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533311.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IERAREDVRTLRHLQLLHQNR"
     gene            complement(1187..1675)
                     /locus_tag="QIE41_004535"
     CDS             complement(1187..1675)
                     /locus_tag="QIE41_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533312.1"
                     /translation="MKLVLTLEADSINVQALNMGRIVVDVDGVNLAELINHVAENGYS
                     LRVVEESDQQSTCTPPPFTALAGIRCSTAHITEKDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(1672..2049)
                     /locus_tag="QIE41_004536"
     CDS             complement(1672..2049)
                     /locus_tag="QIE41_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDS3533313.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNDITLGKHPEAKQ"
     gene            complement(2139..2507)
                     /locus_tag="QIE41_004537"
     CDS             complement(2139..2507)
                     /locus_tag="QIE41_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDS3533314.1"
                     /translation="MSDTFSGTTHPDDNRGRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADHAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGCVYLAVYPTPETKK"
     gene            complement(2582..2803)
                     /locus_tag="QIE41_004538"
     CDS             complement(2582..2803)
                     /locus_tag="QIE41_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDS3533315.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTSREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(2866..3342)
                     /locus_tag="QIE41_004539"
     CDS             complement(2866..3342)
                     /locus_tag="QIE41_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDS3533316.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(3358..3843)
                     /locus_tag="QIE41_004540"
     CDS             complement(3358..3843)
                     /locus_tag="QIE41_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDS3533317.1"
                     /translation="MTTFSHSSTTPSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTIHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(3935..>4723)
                     /locus_tag="QIE41_004541"
     CDS             complement(3935..>4723)
                     /locus_tag="QIE41_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDS3533318.1"
                     /translation="NQIRRDRPLTREELMQVVPSVFGEDKHTSRSENYTWIPTITVLE
                     SLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIILLNSHDGTSSY
                     QMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQ
                     SLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENML
                     KGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         1065 a         1210 c         1305 g         1143 t
ORIGIN      
        1 caattatacc cgcagtccct tttgatatcg aaagggaatc acactcggga gcaggctgag
       61 agcacagaag gaaagtacct ggctattata tcgttaattc tgtcaatcag ttccagcctt
      121 ttgaaagtga tgtgtgcaga tcccttctta aagtatttaa tgatgaacat ctcgtcttca
      181 ttgcgctctt taccctgaac ggactggatg tgaccatcca gacgaatagt gatgttttcc
      241 cggttgtcgg ggatcggttt gccgctgaac agatgtagca ttctttccag gtcgacgagt
      301 cggtcagtct gttgcccggt gataagatga aacccccatc ggtcccacct cactaggttg
      361 ttgacgataa ttttactgcc aaatttgcag gggcaattgg ttttgtaatt ccagttcagt
      421 cccctgaaga cgttgatcac ccctcgctca aacacctcat ccttattctg gtgcagttgt
      481 tcaaatgtgc tcagaatgtt cgcttcactg atttccggaa aattatcgta ttccagtgac
      541 cggtaccatg tatcacgggc ctgcgcatcc attaatgaca gcatcccgga acgttgcatc
      601 aggtcacgcc agatttcgcg atcgagattg cgggtgatgg ctttcattgc tgtttcaggt
      661 ttatccatca gccagcagcc acagcgaaaa tcctgtttca tggcccagtc ccgggcggtt
      721 ttacctccaa tgctttccgt cagaactgag atgtcagcca gttgccggat aagtgtgtcg
      781 atttgcagca gtgtactttt tcgtacagcc agaatgcggc agatactggt tgagcagata
      841 acgtccgtat gcccgttgac cacttccgga cgggaggctg tgatatcaga cataatcgat
      901 attccttttt gcagataaat gaaaacgcca gccctgttca ggctggcgtc agtggaatga
      961 agtctggtga gtaatattta ccggttctgg tgcagaagct gcagatgacg aagagtacgg
     1021 acatcctccc gggcccgttc gatacagggc aacagtgctg ccgaatccgt tttatcctca
     1081 tcgttgtcaa agataattcc cgattcgcag tcgatattgt cctgcagcca cacaatcaga
     1141 atatccagtg ctgtttccgt ggttaatgat ttcatgttgt tcctggttac cagtcgaaag
     1201 tgggcaggtc aggcagacat tcagcgctgg catccagatg aatgagactg acgccataac
     1261 gccggataag tgtgacaacc agacggcgga acgtttttga cagtcccagg cgtttaagcc
     1321 gcagaaccgg gtatgaccac gcatcggtac gtaacagata tccgctacct gtaaaatgaa
     1381 tccattctga ttcgccgaag tcattcgtct gatgtgacag cgagtacagc caggcgttgt
     1441 ccttttccgt gatatgtgcg gtactgcagc gtatgccggc aagggccgta aacggtggtg
     1501 gtgtgcaggt tgactgttgg tcggattcct ccaccacgcg gagtgaataa ccgttttcag
     1561 cgacatggtt aatcagttca gcgagattca caccatcgac gtcaacgaca atgcgcccca
     1621 tgttcagtgc ctgcacgtta atgctgtcgg cttccagcgt caggaccagt ttcattgttt
     1681 cgcctccgga tgcttaccca gggtaatgtc atttaccgtt ctgtagttgt cgcgggtcat
     1741 caggccggtt gcccggcgtg cccggaggat atcaatactg tttattaact gagaacgggt
     1801 acaggcactg aattccggct ggtcggtacg caccagtgcg tatttttcaa cgagaaagtt
     1861 caccgcgtca cacagtgaaa tgcctgcctc aatatgctgc tcaatcacac gttcatcggc
     1921 gaacggtgtg tcattcaggg taaggccgta atgctggccc agtagtcggg agagcagagt
     1981 ctgccagatg gtgacgggag acgggcagct cgatgcctcc cgtacgtgtg tatcgggtaa
     2041 tgttttcata ctgaaggttt tcctgatatg cagagatgaa aaatggaaaa gtggcgtggt
     2101 gaaagcacca ggccggagca gaaggttatt ccggggggtt actttttcgt ttcgggtgtc
     2161 ggataaacag ccagataaac gcaaccacag ctgccgaggg tatcggcttc gcaggtcagc
     2221 ccttttgcat acagcgtgac ggtatgctga tggcggggat tcagttcacc gctggtgagc
     2281 atgagttcca gttgtttcat cagcagcgga aaggcctggt ccagatggta tgcatccgcg
     2341 tcgctgaact gccctctgat accggcgtgg tcggcaaggt aatgcaactg attgccctcc
     2401 tgcaccagac gtgccccgaa acagggcgtc acggtgcagg gcagtcccca ccaggggcgg
     2461 ccgcgattgt cgtcgggatg agttgtcccg gagaatgtgt ctgacacgat aaagtcctca
     2521 cagaaaatca gttaagaatg ctccggtggc agcgataatt ctgctcatca tttctcctga
     2581 ctcagttcag agtgacgctc atcagccgga catacggacc aaaactgtcc ttacggcgtt
     2641 ctgcaaacac ggccagcaca ccgggaatat cctgcacttc acgactggta tacgcttcag
     2701 cactgccgtg ccagcggtat ttgccggtac agaacggaaa aagacgggat gccggatgct
     2761 gacggtgaat acgcatggct tcgccacggg tgatgatttt catagtggga tacctctgaa
     2821 gacagaagat aaaagtgaaa acaggtgtga tgtggttgtg acgggttaaa gcagaccatg
     2881 ttcggcaaag gagaaaacct ggctgccacc gactatcaga tggtccggca cccggatatc
     2941 caccaggccc agtgcctgta ccagacgttc cgtgataagg cggtctgcct tgctgggtgt
     3001 gacttcaccg gacgggtgat tgtgtgccag caccacagca gcggcattgt gatacagggc
     3061 gcgtttaatc acttcacggg gatggacttc cgtgcggttg atggtgccgg tgaagagggt
     3121 ttcaccggca atcagctgat tctggttgtt cagatacagt acccggaact cttcacgctc
     3181 cagtcccgcc atgttcagaa tcagccattc ccgtgccgca cgggtggagg tgaaggccac
     3241 gccgggttca tgaagatggc ggtccagggt tttcagggcc cgcagaatga gactgcgctc
     3301 gccgggcgtc atctctccgg gcagaaagga aagttgttgc attgtgcttc tctccattca
     3361 gtcgatgatg cgcataatgg cgctgcattc cggatgctgc agggcgtaat cccgcaaccg
     3421 gtaataatgg atcgtcatgg cataacactc cgtacgacag gcatgatgac tgtacgtcat
     3481 cagacaggcg gcaatgccgg cggcttccgg gctcatttca gcgcggttac cgttcatggc
     3541 attgaacagt acccaggttt ctccatcatc ttcatccggt tcgggcgcca taaatgcgcc
     3601 gccgttattc agggtgtaca gattccagat accaccgcag tagtcttcac acagacggtc
     3661 catccagccg aagacacggg gctccagggt cacccactgt ggaatgaggc caaagtgctg
     3721 cggccagaag ctgatgcgct gttcatcagg gactatggtg gcaaccagtt gagactggtt
     3781 attccctgat gcagtggtta cggaaacaga aggtgtggtg gagctatgcg aaaaggttgt
     3841 catgagatga ttccttataa aaagtaaatg aatggaagaa tgcctgggga ctggtacaga
     3901 cgtgagtcag ggctgtactt tcagggaaac agcatcagcg catgctctcc agcagcgttt
     3961 ctgccatcac ccacaatgcg cgattgagct taatgtccgt gtcgatgctg tgaatggcac
     4021 gggtgtggat acgttttcct ttagcgctgc gaccggaaat cccgcctttc agcatatttt
     4081 cctggatggt ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgtggcg
     4141 tcagaatgtc ggcggtggtg acgggctgat gttcgtcacc ataacggtaa gtcagtgccg
     4201 cctgtgccag cgcctggcgt gccggtggcg gcaggaccag cgactgcatg gcatcacgct
     4261 tttcctcaat ccggtcaaaa acgcccacca cctcgtaagc cccttcaatg actctgtcca
     4321 ccacatttcc ccggtgcgga acacgcactt cccccagaga ctgaccacag acgcacccgt
     4381 tctggcagac gaacctgaag taacccggca gcatctggta gctggaggta ccgtcatggg
     4441 agttcagcag gatgatttca gggacatgtt gtccgtttat ctccccgtca cggcgcagac
     4501 gcagcatgtg tttggtatac ccccggcggc ccgggtcacg tacgcgggtc tggcaggcaa
     4561 agaatggctg aaaaccttcc cgctgcaggc tttccagcac tgtgatggtg gggatccagg
     4621 tatagttctc gcttctggat gtgtgcttat cttccccgaa tacgctgggg acgacctgca
     4681 tcagttcttc gcgagttaag gggcggtcac ggcgtatctg gtt
//
LOCUS       DAOTRK010000238         4510 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.241, whole genome shotgun sequence.
ACCESSION   DAOTRK010000238 DAOTRK010000000
VERSION     DAOTRK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.241"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(300..449)
                     /locus_tag="QIE41_004542"
     CDS             complement(300..449)
                     /locus_tag="QIE41_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HDS3533319.1"
                     /translation="MESKLKQKVNEKKSAVARPWERKLWADSDGPCCFTKRLGRQAEL
                     GSLRT"
     gene            complement(497..943)
                     /locus_tag="QIE41_004543"
     CDS             complement(497..943)
                     /locus_tag="QIE41_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturase"
                     /protein_id="HDS3533320.1"
                     /translation="MMINEAHAQATAASGRGDGQYPSGLHDGAEISTAAGGQTKAEVP
                     LTMEAVITRENLMLAYQRVVENKGVSELKPWLKKNWRSVWQVLIDGNYQPRAIRRMDI
                     PKPDGGVRTLGIPTVVDRLIQQAIAQQLSAIVDTASAATQTTATST"
     gene            1309..1536
                     /locus_tag="QIE41_004544"
     CDS             1309..1536
                     /locus_tag="QIE41_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HDS3533321.1"
                     /translation="MEDFHLLVVLQPPQLKNSASHGATRHARRTYAASADVNGTRNIL
                     AAGHAVLACKEMVQSGLLFKQEPTEMIPAIA"
     gene            complement(1589..2950)
                     /gene="dcuC"
                     /locus_tag="QIE41_004545"
     CDS             complement(1589..2950)
                     /gene="dcuC"
                     /locus_tag="QIE41_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HDS3533322.1"
                     /translation="MIKVVIALLVIVIVARLILKGYRAEPVLFIAGLALMVCTWFTGW
                     GTVLPKGVSGTGISFLDPFEVMRNLFSTRAADLGLMIMTLMGFAHYMDHIGANEAVVR
                     VVTRPLRTLRSPYVLLFFSYLFASLLQLAIPSATGLAVLLMGTMFPIMLGLGLSAASA
                     AGVIATSLGVAYTPTAIDAIRGSEAVNMDVVEYVVYHQGPAALATVLIVGISHFFWQK
                     HCDRKAGTLPHEIGTTTVIKAGSTPAYYALLPMLPILMAVGSSEIFVTGINLNIITIV
                     LISMAICMLIEWVRKCDLKAVCDGFTHFLKGMGTAFTGVVGLLVAAGVFAHGIKSIGA
                     IDQLILMAEHVGLPPFAMGIVFALVTLAAAVIMGSGNAPFLAFVELIPQIAASMGVNA
                     ISMILPMQQASHMGRAMSPVSGVVIAVSSGANITPFEVVKRTALPLIVGFVFHSAIIG
                     IFY"
     gene            complement(2947..4203)
                     /gene="pepT"
                     /locus_tag="QIE41_004546"
     CDS             complement(2947..4203)
                     /gene="pepT"
                     /locus_tag="QIE41_004546"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDS3533323.1"
                     /translation="MDIVERFINYTKINTTTSRENGAKGIMPSSPGQMKLAKLLVSEL
                     EALGMEDIILRENAIVTATLPANTDETIPVVSFFGHLDTSAEQTADTKAQRLPYNGGD
                     LCLNPELNIYLRESEFPELKNYIGDDLIVTDGTSLLGADDKAALAAIMNALQFLISHP
                     EIRHGEVKVGFVPDEEQGLRGAKAFDVSEFGADFGYTLDCCGIGEFVYENWNAGDAEI
                     IFTGQSAHPMSAKGKLKNSLLMAHKFISMLPGGEAPEYTEGREGYYWVKQLQGNSART
                     VLKLDIRDFSEEGYHARKTFVRQLAESACALWGEGSVICQLSDRYANVFNSLQGEGHY
                     PIDIALRAYQRCGITPTPVAMRGGYDGAVLSQKGLPCPNIFTGAHNFHSIYEYLPVRS
                     LRAASDVVIAIIQETFNGFTTGNRES"
BASE COUNT         1171 a         1204 c          975 g         1160 t
ORIGIN      
        1 caatataaat atttaaactt tttccattat gtttatttga cttctatcaa aaaaaattat
       61 gctaattcga ttttgcaaaa tttaattgca cgcaaataat atgcacatat tttttagtgt
      121 aatgagtgac ttttatggat aggttcccgc tgacctctat caccatactt ccccccgctt
      181 tcagtaggac atccgtgtct gactttatat cgagatttca tgtggaagag ataaatgccg
      241 gaccttgaga aagaaatata actccccagt ctcgttgaac cctcttgttg atccactttt
      301 caagtacgta acgatccaag ttccgcctgc cgtccaaggc gtttggtgaa gcagcacggt
      361 ccgtcactgt cagcccacaa cttccgctcc cacgggcgcg ccaccgcgct cttcttctca
      421 ttcaccttct gtttcagttt gctctccacg aacgccctga tgtccttcag cagatggtcg
      481 cccgctttcc ggctgcttac gtagatgttg cagtcgtctg cgtagcggca gaagctgtgt
      541 ccacgatggc gcttaactgc tgcgctatcg cctgctggat aagcctgtcc accaccgtcg
      601 ggatgcctaa ggttctcacg ccgccatccg gcttcgggat atccatccgg cgtattgccc
      661 gcggctggta gtttccgtca atcaatacct gccatacgct gcgccagttc ttcttcagcc
      721 acggtttcaa ctccgacaca cctttgtttt ccaccacgcg ctgataagcc agcatcaggt
      781 tttctctcgt tatcaccgct tccatcgtca gcggcacttc cgctttcgtt tgcccacccg
      841 ccgccgtgct gatttcagcg ccatcatgca gccctgacgg atactgtccg tctcctctgc
      901 cgctggccgc agttgcctgc gcgtgtgctt cattaatcat catcgggttc ggatgtccta
      961 atcacagtta atcatgttcg gcccttcatg ctgccggttt tgcagcacta ctacggcgtc
     1021 ggctgacttc tgccgcccca tcccggcgcc tcacggcacc ggtagcacac tggcagggcg
     1081 acagatctcc cggggtaatt cacgcgactt tcctgcttat gcctgtcggc tttacgtcac
     1141 agcgttccgt gtgagtatcg ggctttggtg atttgtgcca ccttaccctg ctgcgccgcc
     1201 ttaaccgctt cctgttcgtc aggccagcat tttgcatccg gcttccttca gatttcacct
     1261 cgcgatgaac acccttgcct tccgctaaca cttcccctca ccgggtgtgt ggaggacttt
     1321 cacctcttag tcgttcttca gccaccacag ttgaagaaca gcgccagtca cggcgctacg
     1381 cgccatgccc ggcgcacata tgcagcgagc gccgatgtaa acggcacccg taatatttta
     1441 gctgcagggc acgccgtact tgcctgtaaa gagatggtgc agtcaggcct tttgtttaag
     1501 caggagccca ccgaaatgat tccggcaata gcctgattag caggaatcca catccttctg
     1561 ggcgtggagg atgacaatct aaaacaagtt aataaaaaat accgataatc gcagagtgaa
     1621 aaacaaaacc aactatcagg ggtaaagcag tgcgttttac cacttcgaag ggagttatat
     1681 ttgctccact ggaaaccgct ataaccacgc cagatacggg agacatcgca cgccccatat
     1741 gggaagcctg ctgcataggc aggatcatcg atatggcatt cacgcccatg ctggcagcga
     1801 tttgtggtat cagttcaaca aaagccagaa atggcgcatt gcccgatccc ataatgacag
     1861 ctgcagccag tgtaaccaga gcaaaaacga tgcccatcgc aaaagggggt aacccaacgt
     1921 gctctgccat caaaattaat tgatctatcg cgccaatgct tttaatccca tgagcaaaca
     1981 cacctgcggc caccagtaag cccaccacgc cggtaaatgc tgtccccatt cctttcagga
     2041 aatgggtaaa cccgtcacat acagctttca gatcacattt acggacccat tcgatcagca
     2101 tgcaaattgc cattgagata agaacaatcg taatgatatt gagattaatt ccggtgacaa
     2161 aaatctccga tgagccaacg gccatcagga tcggtagcat aggtagcaga gcataataag
     2221 ccggcgtact gcctgctttt atgaccgttg tcgtaccaat ttcatgaggt aatgttccag
     2281 ctttacgatc acagtgtttc tgccagaaaa agtggctgat accgacaatc agaacagtcg
     2341 ccagagcagc aggcccctga tgatagacaa catactccac cacatccata tttaccgctt
     2401 cagagcctcg tatggcgtca atggctgtcg gtgtgtacgc cacaccaaga gaggtggcaa
     2461 ttacccctgc ggcagacgct gctgacagcc cgagtccgag cataatagga aacatggttc
     2521 ccatcagtaa aacagcaagt ccggtagctg aggggatagc cagttgaaga agactggcga
     2581 aaagataaga gaaaaaaagc agaacatagg gggaacgtaa tgtccgtaat ggccgggtta
     2641 ccacacggac aaccgcttca ttcgcaccga tatggtccat gtaatgcgca aatcccatca
     2701 gggtcataat catcagaccg agatcagccg cacgggtact gaataaattg cgcatgactt
     2761 cgaaaggatc aagaaaacta attcctgtac cggacacgcc cttcggtaac actgtgcccc
     2821 aaccggtaaa ccaggtacaa accatcagcg caagcccggc aataaaaagc acgggttctg
     2881 cacgatatcc tttcaggatt aagcgagcaa ctatcacgat aacaagaagc gcgataacaa
     2941 ctttaatcat gactcacgat tccctgtggt gaacccattg aatgtctcct gaatgatggc
     3001 aataaccaca tcacttgccg cccgaagtga acggactgga agatattcat agatagaatg
     3061 gaaattatgt gcgccggtaa aaatgttcgg gcagggtaat cctttttgtg aaagaacagc
     3121 gccatcatat cctccacgca tcgccaccgg tgtcggagta ataccacatc gttggtaagc
     3181 tcgcagggca atgtcgatgg gataatgccc ctctccctgc agactgttaa agacattggc
     3241 gtaccgatcg ctcagttggc agatcacgct tccttctccc cataatgcac aagcactttc
     3301 tgcaagctgg cgtacaaatg tcttccgggc gtgatatcct tcctcgctga aatctcgtat
     3361 atccagtttt agtacagttc tggcactgtt tccctgtaac tgtttcaccc aataatagcc
     3421 ttcgcgtcct tctgtatatt caggcgcttc tcctcctggc aacatcgaaa tgaatttgtg
     3481 agccatcaaa agagagttct taagcttgcc tttcgctgac atagggtgtg cagactggcc
     3541 ggtaaagata atttctgcat cacccgcatt ccagttttcg taaacaaatt ctccaatacc
     3601 gcagcaatcc agagtgtagc caaaatctgc gccaaactct gaaacatcga aggctttggc
     3661 cccccgtaac ccctgctctt catctggcac aaacccaact ttaacttctc catgcctgat
     3721 ttcaggatga gaaatcagaa attgaagtgc gttcattatg gccgccagcg ccgctttatc
     3781 atcggccccc agaaggctgg taccatctgt cactataagg tcatcgccaa tataattttt
     3841 gagctccggg aattcactct cacgcaaata aatattcagt tcagggttca gacagagatc
     3901 gcccccatta taaggtaatc gttgcgcttt tgtatcggct gtctgttcgg cactggtgtc
     3961 cagatgtcca aaaaaggaaa cgacggggat tgtttcgtca gtatttgcag ggagtgttgc
     4021 tgttacgata gcattctctc tgagaataat atcctccata cccaatgctt caagttcact
     4081 caccaacaat ttcgcaagtt tcatctgacc aggagatgaa ggcatgatcc cttttgcgcc
     4141 gttttctcgt gaggtagtgg tattaatttt tgtataattg ataaagcgtt cgacaatatc
     4201 cattgagatg ccttcttcaa cattttccag attttaggga cagtttatta acaccttctg
     4261 actctgaaga acgtgaattt atgctatgtt caggctgtca tttttccaag agagaaatga
     4321 taattatgaa gagcctttgc atgattgaac ataaaataca gactccatta cagtatggcg
     4381 tttttttagc ttgtgatgat taatgccgtt acaccctgaa agacgattta gtgatcaaat
     4441 catctaaaaa taattaatca taatggatgt taaaatccat gatctaactc aaattacaaa
     4501 ccattaaagc
//
LOCUS       DAOTRK010000239         4490 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.242, whole genome shotgun sequence.
ACCESSION   DAOTRK010000239 DAOTRK010000000
VERSION     DAOTRK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.242"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1188
                     /locus_tag="QIE41_004547"
     CDS             <1..1188
                     /locus_tag="QIE41_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
                     /protein_id="HDS3533324.1"
                     /translation="KSNGIFDFIENDLVFDNDSTNKIKMNHIKTMLCGHFIGIDKLER
                     LKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTTQMFRDINFKVFKKLLTKSKLTSI
                     TIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQFAFCDELKAYVTGFVRSGNTYTA
                     NGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIETIESLGCKYLIKAKSYSTLTSQ
                     ATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRVLKPEKERAQLSLLEGSEYDY
                     FFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGHLLLKSFWANEAVFQMMMLS
                     YNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTARYVIMKLSENYPYKGVYEK
                     CLV"
     gene            1444..2319
                     /locus_tag="QIE41_004548"
     CDS             1444..2319
                     /locus_tag="QIE41_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-55"
                     /protein_id="HDS3533325.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(2366..2698)
                     /locus_tag="QIE41_004549"
     CDS             complement(2366..2698)
                     /locus_tag="QIE41_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HDS3533326.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            2653..4047
                     /locus_tag="QIE41_004550"
     CDS             2653..4047
                     /locus_tag="QIE41_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533327.1"
                     /translation="MATVARRCEGVGAGARRSQVLVKKNPRGKVIRELSSEEETAVKT
                     VCGLKKPATMAQHNLANDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSS
                     FNHEHAPECPMYRQLSGNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQTRSAY
                     HNGERSSVRKKRPRLGRLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQE
                     YIRGRSLSEIIHFRPGMSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAV
                     EIHWANGNIQVFRPVRGISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQ
                     LTCPVPVDSKLERNTLTALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPD
                     FIVTARAPDGKTARVVIETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPP
                     FEKHMYGVFMHLRY"
BASE COUNT         1310 a          943 c         1051 g         1186 t
ORIGIN      
        1 aaaagcaatg ggatttttga ttttattgaa aatgacctcg tatttgataa tgactcaaca
       61 aataaaatca agatgaatca tataaagacc atgctctgcg gtcacttcat tggcattgat
      121 aagttagaac gtctaaagct acttcaaaat gatcccctcg tcaacgagtt tgatatttcc
      181 gtaaaagaac ctgaaacagt gtcacggttt ctaggaaact tcaacttcaa gacaacccaa
      241 atgtttagag acattaattt taaagtcttt aaaaaactgc tcactaaaag taaattgaca
      301 tccattacga ttgatattga tagtagtgta attaacgtag aaggtcatca agaaggtgcg
      361 tcaaaaggat ataatcctaa gaaactggga aaccgatgct acaatatcca atttgcattt
      421 tgcgacgaat taaaagcata tgttaccgga tttgtaagaa gtggcaatac ttacactgca
      481 aacggtgctg cggaaatgat caaagaaatt gttgctaaca tcaaatcaga cgatttagaa
      541 attttatttc gaatggatag tggctacttt gatgaaaaaa ttatcgaaac gatagaatct
      601 cttggatgca aatatttaat taaagccaaa agttattcta cactcacctc acaagcaacg
      661 aattcatcaa ttgtattcgt taaaggagaa gaaggtagag aaactacaga actgtataca
      721 aaattagtta aatgggaaaa agacagaaga tttgtcgtat ctcgcgtact gaaaccagaa
      781 aaagaaagag cacaattatc acttttagaa ggttccgaat acgactactt tttctttgta
      841 acaaatacta ccttgctttc tgaaaaagta gttatatact atgaaaagcg tggtaatgct
      901 gaaaactata tcaaagaagc caaatacgac atggcggtgg gtcatctctt gctaaagtca
      961 ttttgggcga atgaagccgt gtttcaaatg atgatgcttt catataacct atttttgttg
     1021 ttcaagtttg attccttgga ctcttcagaa tacagacagc aaataaagac ctttcgtttg
     1081 aagtatgtat ttcttgcagc aaaaataatc aaaaccgcaa gatatgtaat catgaagttg
     1141 tcggaaaact atccgtacaa gggagtgtat gaaaaatgtc tggtataata agaatatcat
     1201 caataaaatt gagtgttgct ctgtggataa cttgcagagt ttattaagta tcattgcagc
     1261 aaagatgaaa tcaatgattt atcaaaaatg attgaaaggt ggttgtaaat aatgttacaa
     1321 tgtgtgagaa gcagtctaaa ttcttcgtga aatagtgatt tttgaagcta ataaaaaaca
     1381 cacgtggaat ttagggacta ttcatgttgt tgttatttcg tatcttccag aataaggaat
     1441 cccatggtta aaaaatcact gcgccagttc acgctgatgg cgacggcaac cgtcacgctg
     1501 ttgttaggaa gtgtgccgct gtatgcgcaa acggcggacg tacagcaaaa acttgccgaa
     1561 ttagagcggc agtcgggagg cagactgggt gtggcattga ttaacacagc agataattcg
     1621 caaatacttt atcgtgctga tgagcgcttt gcgatgtgca gcaccagtaa agtgatggcc
     1681 gtggccgcgg tgctgaagaa aagtgaaagc gaaccgaatc tgttaaatca gcgagttgag
     1741 atcaaaaaat ctgaccttgt taactataat ccgattgcgg aaaagcacgt caatgggacg
     1801 atgtcactgg ctgagcttag cgcggccgcg ctacagtaca gcgataacgt ggcgatgaat
     1861 aagctgattg ctcacgttgg cggcccggct agcgtcaccg cgttcgcccg acagctggga
     1921 gacgaaacgt tccgtctcga ccgtaccgag ccgacgttaa acaccgccat tccgggcgat
     1981 ccgcgtgata ccacttcacc tcgggcaatg gcgcaaactc tgcggaatct gacgctgggt
     2041 aaagcattgg gcgacagcca acgggcgcag ctggtgacat ggatgaaagg caataccacc
     2101 ggtgcagcga gcattcaggc tggactgcct gcttcctggg ttgtggggga taaaaccggc
     2161 agcggtggct atggcaccac caacgatatc gcggtgatct ggccaaaaga tcgtgcgccg
     2221 ctgattctgg tcacttactt cacccagcct caacctaagg cagaaagccg tcgcgatgta
     2281 ttagcgtcgg cggctaaaat cgtcaccgac ggtttgtaat agcggaaacg gaatggggaa
     2341 actcattccg tttttgttta tcgccttaga cggcaaaagt gctgtcgccc acctgcgctt
     2401 gcgcatacca ggccataagc tccgtggttc ctggttctcc ttccgctgga gcccagtgcg
     2461 catagtcatc ggcagccacg ggttgatagc caccgtgttt tacttcaaaa attatgccac
     2521 cggtatccag cgacagcacg gcatgccagg ttcctgcggc catctccagc accgtacagg
     2581 tttcccccaa tatcgcccga tgggtgacgg taccccgatc gtcaaaattc agcaccacga
     2641 aacgacccct taatggcaac agtagctcga aggtgtgagg gtgtcggtgc gggcgcacgt
     2701 aggtcccagg tacttgtaaa gaagaatccc cggggcaaag tgattcggga gcttagcagt
     2761 gaggaagaga ctgcagtaaa gacggtctgt ggactgaaaa agccagcaac catggcacaa
     2821 cataacctgg ccaatgatct tctccgggaa atgcgtgagt atgatgcatg gttacagtgt
     2881 gactgtattc cgggagattc gccggccatg aattttgctg ctcttaaaaa caatacgggt
     2941 actctttatc tgtccagttt taatcatgag catgcacctg aatgccccat gtatcgtcag
     3001 ttatcaggaa atgaagagga aacatccttc ggagcaagcc ggcatcccgt tagcacccgt
     3061 ataaattaca gaaacttttt accacctgat gacagtaatt ctgttatcag actgcagacc
     3121 cgctctgctt accataatgg tgagcgctcc tccgttcgca agaaacgtcc gcgccttggc
     3181 cgtttactcc tgtcgcttat tgaagatgca gggctgaata aactggattc cctggctaat
     3241 ccccgtatcc ggactaatta tcgtgagtgt cttgatgcca tacggcaggt tacactacag
     3301 caggagtata ttcggggaag atccttatct gaaattatcc atttccggcc aggaatgtct
     3361 gaaaggtctc aggagcggtt aatggaaacg ctggagaatt cagaacgaca ctggcctgcc
     3421 agaagaaaac atatgttctt tcagattttt atggcgcagc acatctgtcg tgatgctgtg
     3481 gaaattcact gggcgaacgg taatattcag gtcttcagac ctgtgcgggg gatcagtatt
     3541 aatggtgaag cgcagggcgg aatacgtccc ccttactggg ttattcttgc tttttgccgg
     3601 agtgccgatg gcagaatcat ctgcagtgaa gggtatgctc atgctcttta tcaactgaca
     3661 tgtccggttc ctgtggacag caaactggaa cgaaacacgc tcactgctct tctgaatgtg
     3721 gccagctggc ttaaaagaaa gccaggtacg ccggaattaa gtctggaaag gcccctgttt
     3781 gatacagaag tttatgttaa tggtgaaaag aaatatgtac tgccggattt cattgtcaca
     3841 gcaagggctc ctgacggaaa gacggccaga gtggtcatcg aaacgatggg atatgaagac
     3901 agtgattact gcgcgagaaa atccaggcag cataccggca tgaagcagat tggtgttctg
     3961 cataccgatc caccgaaatg gctggataac gatcatcccc cttttgagaa acatatgtac
     4021 ggtgttttta tgcatctcag gtactgagat attttgtggc tcagttctgt aacttttccc
     4081 gtaacattgt ctgttgttac gggaaagtcc ggtttttgta ttgcaccaga gaatactcag
     4141 actgtgatgc tgccacagcg tcagcaggct ttctgaacgg tgtaacatca cttcatgtta
     4201 atgataatca ctatcattaa atcttgacat gccattttct ccttaataaa ttaatactgt
     4261 atatgtatcc atatacgtaa gcagttaatt catttgtttt cctcagagga tgaaggagat
     4321 accgaatgtc tgatcctgta cggggttcag tgagttagct ggcctggatg taaccactcg
     4381 ttcaactcaa ttctcttagc gtgggaaaat ttccgctttt ccaagcggac ccctattaat
     4441 atttgttttg ttttaggttc tattcaacat aggtcatcct aaaatacatt
//
LOCUS       DAOTRK010000240         4394 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.243, whole genome shotgun sequence.
ACCESSION   DAOTRK010000240 DAOTRK010000000
VERSION     DAOTRK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.243"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            390..998
                     /locus_tag="QIE41_004551"
     CDS             390..998
                     /locus_tag="QIE41_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDS3533328.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1096..2487
                     /gene="selA"
                     /locus_tag="QIE41_004552"
     CDS             1096..2487
                     /gene="selA"
                     /locus_tag="QIE41_004552"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDS3533329.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LENEQRFLEMLLK"
     gene            2484..4328
                     /gene="selB"
                     /locus_tag="QIE41_004553"
     CDS             2484..4328
                     /gene="selB"
                     /locus_tag="QIE41_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDS3533330.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLTDAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1008 a         1116 c         1278 g          992 t
ORIGIN      
        1 ccgagcagcg gattgaccgg atggccggac gtcaccccgc cggggcacac cgaacaggcc
       61 acgccggtgg gggcaccgat gcgcaccccg gctgaaccct ggacaatgct accgccatac
      121 tgcgtcatgt cgccctgacg cgccgccggt tttccgctca tctgctcatc cttgttttat
      181 cgggttattt gtctgctgac aggcgaaacc ctacagattt ataagaaagc cttaagtgag
      241 atatctgtca gataagaaca attaggaaaa tatttatatc accatgataa atatgtgatt
      301 tattgggaag taacaaacca tcaacatttt ctgccctttt tcccatcacc aggccatact
      361 gatatttttc ctcgcacaaa ggactcggca tgaaactcgt cggtagctac accagcccgt
      421 ttgtacgcaa actttctatt ctgttgttag aaaagggcat aactttcgaa tttattaatg
      481 aactgcccta taacgcggac aacggcgtgg cgcaatttaa cccgttagga aaagtgccgg
      541 tgctggtgac cgaagagggc gaatgctggt ttgattcgcc gatcatcgct gaatatattg
      601 aattaatgaa tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc
      661 gcaaaattga ggcgctggcg gatggcatta tggatgccgg actggtatcg gtgcgtgaac
      721 aggcgcgtcc ggcggcgcag cagtctgaag atgaattgtt acgccagcgg gagaaaatca
      781 accgcagtct ggatgtgctg gaaggatatc tggtcgatgg cacactcaaa accgatacgg
      841 tcaatctggc gactatcgcc attgcctgtg ccgtcggata tctcaatttc cgccgcgttg
      901 cgccaggttg gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc
      961 gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc
     1021 catctcacgt tacaatccgt ggttatgtta aacgcccttc tccgtgtgag agggccttga
     1081 tcagccaggt ttcctatgac aaccgaaacg cgttccctct atagtcaact tccggctatt
     1141 gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg atacttatgg tcacacccgc
     1201 gtggtggaat tgctgcgtca gatgcttgac gaagcgcgag aagtgattcg tgacagccag
     1261 acgctgcccg catggtgtga aaactgggcg caagaagtcg atgcccgact gacgaaggag
     1321 gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca taccaatctt
     1381 gggcgagctt tacaggcaga agccgcagtg gaagccgttg cgcaggctat gcgttcgcca
     1441 gtgaccctcg agtacgatct ggacgacgcc ggacgcggac atcgcgatcg ggcgctggcg
     1501 cagctgctgt gccgcattac gggagcggaa gatgcctgta tcgttaataa caatgcggcg
     1561 gcggtgttat tgatgttggc ggccactgcc agcgggaaag aggtggtggt atctcgcggc
     1621 gaactggtgg agattggcgg cgcgtttcgt attcctgatg tcatgcgtca ggcaggctgc
     1681 accctacacg aagtggggac caccaaccgc acgcacgcta atgattatcg tcaggcggtg
     1741 aatgaaaata ccgcgctgtt gatgaaagta cataccagta actacagcat tcagggattc
     1801 accaaagcga tagatgaagc ggaactggtg gcgctcggca aagagctgga tgttcccgta
     1861 gtgactgatt taggcagtgg ctcgctggtt gatcttagcc agtacggttt gccgaaagag
     1921 ccaatgccgc aggagttgat tgcggcgggt gtcagtctgg tgagcttctc cggcgacaag
     1981 ttgttaggcg ggccgcaggc aggaattatt gttggtaaaa aagagatgat cgcccgcctg
     2041 caaagccacc cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa
     2101 gccacgttgc gtctttattt acaccctgaa gcactgagtg aaaaattacc gaccctgcgc
     2161 ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac aacgtttaca ggcccccctt
     2221 gccgcacatt acggcgcgga gtttgcggta caggttatgc catgtctttc gcagattggc
     2281 agtggttcgc tgccggttga tcgcctgccg agcgcggcat taacgtttac accccatgat
     2341 ggacgcggta gccaccttga gtcattagcc gcccgctggc gtgaattgcc agtgccggtg
     2401 attggtcgta tttatgacgg acgattgtgg ctggatttac gctgccttga aaatgagcaa
     2461 cggtttttgg agatgttgtt gaaatgatta ttgcgactgc cggacacgtt gaccacggca
     2521 aaacaacctt attgcaggcg attactggcg taaatgccga ccgtctgccg gaagaaaaaa
     2581 agcgcggcat gaccatcgat ctcggttatg cctactggcc gcagccggat ggtcgcgtgc
     2641 ctggttttat cgacgtaccc ggtcatgaaa agtttctttc caacatgctg gcgggcgttg
     2701 gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga cggcgtgatg gctcagactc
     2761 gtgagcatct ggcgattttg cagctgaccg gtaacccgat gctgacagtg gcgctgacca
     2821 aagccgatcg cgtggacgaa gcgcgtgttg atgaggttga acgccaggta aaggaggtgc
     2881 tgcgggaata cggttttgct gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg
     2941 gaattgatgc cctgcgcgag catttgcttc agttaccaga acgtgaacac gccagccaac
     3001 atagtttccg cctcgcgatc gaccgtgcat ttaccgtaaa aggtgccggg ctggtcgtca
     3061 ccggtacggc gttaagcggg gaagtgaagg taggcgattc actctggctg actggtgtaa
     3121 ataaaccgat gcgtgtacgt gcgctgcatg cacaaaacca gccaacagaa accgcccatg
     3181 ccgggcagcg tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag attaaccgtg
     3241 gcgactggct gcttaccgat gcgccgccgg agccgttcac acgggtgatt gtcgagcttc
     3301 aaacccatac accgctgacc cagtggcagc cgctgcatat tcaccacgcc gccagccacg
     3361 tcacgggacg cgtttcgctg ctggaagata accttgccga gctggtattc gacacgccgc
     3421 tgtggctggc ggataacgat cgcttagtgc tgcgcgatat ctcggcccgc aacacgctgg
     3481 cgggggcgcg tgtggtgatg cttaacccgc cgcgtcgcgg taagcgtaag ccggaatatc
     3541 tgcaatggct ggcgtctctt gcacgagcgc agagcgatgc cgatgcgtta tctgttcatc
     3601 tggaacgcgg agcggttaac cttgcagatt tcgcctgggc acggcagctc aacggcgaag
     3661 ggatgcgcga actgctgcaa cagcctggct atattcaggc tggttatagc ttgttgaatg
     3721 cgccggttgc cgcccgctgg cagcggaaaa ttctcgacac attagcgact tatcatgagc
     3781 aacatcgcga tgaacctggt cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg
     3841 aagatgaagc gctggtactg ttgctgattg aaaagatgcg cgaaagcggc gacatcctca
     3901 gtcatcacgg ctggctgcat ctgccggatc acaaagcggg cttcagcgaa gagcagcagg
     3961 ccatctggca aaaagcagag ccactgtttg gtgacgaacc gtggtgggtg cgagacctgg
     4021 caaaagagac gggaaccgac gagcaggcaa tgcgtctgac gctacgtcag gcggcgcagc
     4081 aggggataat taccgcgatc gttaaagatc gttattaccg taacgatcgg attgtcgagt
     4141 ttgccaatat gatccgcgat ctcgatcagg aatgtggttc aacctgcgcg gcggatttcc
     4201 gcgatcgctt aggcgtaggc cgaaagctgg caattcagat tttggaatat tttgaccgca
     4261 ttggctttac gcgtcgtcgt ggaaatgatc atttattgcg cgacgcatta ttatttccgg
     4321 aaaaataagg aaatgattaa ttaagtttta aaataaatta atacaaaatt cttatgaatt
     4381 taaaaaaagc acat
//
LOCUS       DAOTRK010000241         4334 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.244, whole genome shotgun sequence.
ACCESSION   DAOTRK010000241 DAOTRK010000000
VERSION     DAOTRK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.244"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(484..1443)
                     /locus_tag="QIE41_004554"
     CDS             complement(484..1443)
                     /locus_tag="QIE41_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HDS3533331.1"
                     /translation="MIKKPVIGISGCLAGSAVRFDGGHKRADFLMDKLVEWVTFRPVC
                     PEMAIGLPVPRPALRLVRSTQGNIRMCFSHDQNEDVTERMTEFSRSYMDKLKDVSGFV
                     VCAKSPSCGMERVRVYDENGNRGRKDGVGLFTSTLMEKFSWLPVEEDGRLHDPVLREN
                     FVERVFALHELNHLYKEKLSRRELLAFHSRYKLQLLAHSQAGYKDMGPFVAAIHEWAD
                     LESYFEVYRDKLMAILRKPASRKNHTNVMMHIQGYFSNYLSTRQRKELSEVILNYRSG
                     TLPLLAPLTLLKHYLGEYPNDYLLTQNYFDPYPDELALRLMVN"
     gene            1636..2160
                     /locus_tag="QIE41_004555"
     CDS             1636..2160
                     /locus_tag="QIE41_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDS3533332.1"
                     /translation="MKLWPVLTGIALSFTLIACKAPTPPKGVQPITNFDANRYLGKWY
                     EIARLENRFERGLEQVSATYGKRNDGGIRVLNRGYDPTKNKWSESEGKAYFTGDTKTA
                     ALKVSFFGPFYGGYNVIKLDDEYKYALVSGPNREYLWILARTPTIPDKVKADYVRTAQ
                     KLGFNVNELLWVKQ"
     gene            complement(2316..2693)
                     /locus_tag="QIE41_004556"
     CDS             complement(2316..2693)
                     /locus_tag="QIE41_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDS3533333.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVGGMTFMALARKHGRSDCWVGRMLQKA
                     EGVVEGMLMVLDLRLEMDADCSK"
     gene            complement(2779..2919)
                     /locus_tag="QIE41_004557"
     CDS             complement(2779..2919)
                     /locus_tag="QIE41_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HDS3533334.1"
                     /translation="MMFEFYMAELLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2916..3278)
                     /locus_tag="QIE41_004558"
     CDS             complement(2916..3278)
                     /locus_tag="QIE41_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDS3533335.1"
                     /translation="MNTYNITLPWPPSNNRYYRHNRGRTHISAEGQAYRENVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVVKGGKLELTITEQGDE"
     gene            complement(3275..3565)
                     /locus_tag="QIE41_004559"
     CDS             complement(3275..3565)
                     /locus_tag="QIE41_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDS3533336.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3558..3728)
                     /locus_tag="QIE41_004560"
     CDS             complement(3558..3728)
                     /locus_tag="QIE41_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533337.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRRPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3728..4183)
                     /gene="ybcN"
                     /locus_tag="QIE41_004561"
     CDS             complement(3728..4183)
                     /gene="ybcN"
                     /locus_tag="QIE41_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HDS3533338.1"
                     /translation="MNLPQDGIKLHRGNFTAIGRQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(4180..4281)
                     /locus_tag="QIE41_004562"
     CDS             complement(4180..4281)
                     /locus_tag="QIE41_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533339.1"
                     /translation="MRTYNLNSLLSSQMQKCTCDSLHPTFDLCGGEA"
BASE COUNT         1232 a         1041 c          876 g         1185 t
ORIGIN      
        1 tatttgatga aaaaaatctt ttaaatttta atcaatgaga acagcacttc cttttttaaa
       61 taaaatatct tctttaccaa ttcggacatc aaacgagttc aacaccaaaa tgttagaaca
      121 tatgtatggc atattatcca cctgatatca ttggggttac accaagtaag tataggtgga
      181 aaatcaatat tcgccagttc aacaataagg aaaatctcat tgcatcacaa gtataaaatt
      241 atgtatttaa ctcacaaaga caaattatta aaccaatctg ttatattata tatagctgcg
      301 tggaatcata atattatata ttttgactgg catgtttacc aactttaagt tgcatctcaa
      361 tggtttcttc agcgtaaaca gagtttttat acaaactgac actctgggta tcatagtgta
      421 gtttttacga ttgtaaatat cctgcatgca ggaactcatc cttttggatg atatcgcata
      481 caattaattt accatcagtc ttagagccag ttcgtccgga tagggatcga agtaattctg
      541 tgtaagcaag taatcattag gatactcacc cagataatgc ttcagcagag tcaacggcgc
      601 aagaagaggt aatgtgccag aacgatagtt aagtataacc tcgctcaact ctttacgctg
      661 gcgtgtactt aagtagttac taaaataccc ctgtatatgc atcatcacat tcgtgtgatt
      721 tttacgtgat gcaggttttc tgagaatcgc catcagctta tcacgataca cctcaaagta
      781 tgattcaagg tccgcccact cgtgtattgc agccacaaat ggtcccatat ctttatagcc
      841 tgcctgacta tgcgccaaca actgaagctt ataacgacta tgaaaagcta ataactctct
      901 tcttgataat ttctccttgt aaaggtgatt gagctcatgc aaagcaaaaa ctctttcaac
      961 aaaattctca cgaagcactg gatcatgtaa tcgcccatcc tcttcaaccg gtagccagga
     1021 aaacttttcc atcaaagtgc tcgtaaatag tcccactcca tctttacgac ctcgattacc
     1081 attttcatca tagacacgca cgcgctccat gccacagctg ggagatttag cacaaaccac
     1141 aaaccccgat acatccttta atttgtccat ataagaacga ctaaactctg tcattctctc
     1201 tgtcacatcc tcattctggt cgtggctgaa acacatccgt atatttcctt gcgtcgagcg
     1261 cacaagacgt agagcaggac gcggaactgg cagccctata gccatttccg gacatactgg
     1321 tctgaatgtt acccattcca ctaatttgtc cattaaaaag tcagctcttt tgtgaccacc
     1381 atcaaaacga acagcagaac cggccagaca accgctgatt ccaatcacag gtttttttat
     1441 catatcctcc cccttgacta attcattaac acataaactg tgtagtgcac ggaataaatt
     1501 gcctttctgg cgtcatcact gacaattttt ctgttatgga ctattcctaa tatagtatga
     1561 aagttcttta agtgatcggt cgtaatcatc tatctttcat acttactctc aactatcaaa
     1621 agtacaggat ttattatgaa gttatggcct gtgttgactg gcattgcact ctctttcact
     1681 cttatagcat gtaaggcccc gacaccacct aaaggtgtgc agccgattac aaattttgac
     1741 gccaaccgct acctcggaaa gtggtatgaa atagctcgcc tcgagaaccg gttcgaacgt
     1801 ggtctggaac aggtcagcgc tacttatgga aaacggaacg acggagggat tcgtgtactt
     1861 aaccgtggat acgatccaac gaaaaataaa tggagcgaga gcgaaggtaa agcatacttt
     1921 actggagata ctaaaactgc agcgttgaag gtttcgtttt ttggcccctt ctatggtggc
     1981 tataatgtaa tcaaactgga tgatgagtat aagtatgctc ttgtcagtgg tccgaacaga
     2041 gaatacctat ggattctggc aaggacccca actattccag ataaagtaaa agcagactat
     2101 gtgcgaaccg ctcaaaagtt gggattcaat gtcaatgaat tattatgggt taaacaataa
     2161 aatccctacc cgaaataata cttattagaa aaaaaccagc ctttggggag gctggctaaa
     2221 tcaggaaaca agctgttata tgataataac tacgttgcga ttccaacatt taaaatgtta
     2281 gactaatgag aatcagacag caacttttcc tttaattatt tcgaacaatc agcatccatc
     2341 tccaatcgga gatccaacac catcagcata ccctccacta cgccctcagc tttctggagc
     2401 atcctgccaa cccaacaatc agatcgccca tgcttacgtg caagcgccat aaaagtcatg
     2461 ccgccgacat aatagtccac caataaatca tgcaaatcgc tgttgttctt tttcagacgg
     2521 gccatgcacc cgcaaatgat catcgcgtca tcgtcacaac attgtgggcg agattttact
     2581 tttgaaggaa ttaatccctt aaaaccggcg gcaatggacg accaggtcac atcttcatga
     2641 ttattagccg cccacgctcc ccaacgctca agaaccatct gaatatcacg catcaactta
     2701 ctccacaaaa ctcagaccag aacgccaatt acaagcaaaa atcaacaaaa cagtattagt
     2761 tgattgttat ctctgacttc atactcctgc tcctgtcagg gttttggcgt aattcttcag
     2821 tattcggtaa tcggtcaaaa cagaaccagg gaaacgatat aagcgcaggc gcatccagcg
     2881 gtggcgaaga agttctgcca tataaaactc aaacatcatt catctccctg ttcagtgatg
     2941 gtcagttcca gctttccacc tttgacaacc ggcatcttca caacgcggta atcaacgacc
     3001 tgagcatcat ccaaccagaa acctgctttg gtgagtgcgt caaaagcggc cttttgtaga
     3061 ttatccaggt cacggcgacg gcgatccggc atgtgacact caatgcggat tttcacaggc
     3121 atagccaggc cgatatccag cattgcgttt ttaatgattc gggcgacgtt ttcgcggtat
     3181 gcctgcccct ctgcgctgat gtgcgtgcgc ccgcgattat ggcggtaata gcgattattg
     3241 ctcggcggcc agggtaatgt gatattgtag gtattcacgc cttaattacc ccctctttca
     3301 gccagataac ctgcgttctc gccatacctt ccagcgcgca ttcttttgca tatccagcgt
     3361 caacaaaatg cgtgcgacgg tcgatctcgt cgtgacaggc agaacatgca atggtggcaa
     3421 tcaggtctgg cggtttgata ccggtaccgc acaatccagc cagccggata tgtgccagta
     3481 cagacgtttc aggattgcca ttacatacgc cagggattct tacctggcat tcccgaccac
     3541 gcgctgcttt tctcaaatca gccatgattc ctccttgctg ccagtcgcaa ccatttttta
     3601 tcaaccaggc tggcggtata tccgagcagt gttggtattt cggatggttt cagctcaggc
     3661 ctacgcttac gacgatttgg tactttgtag atgtgtccgt tcatgacacg aataagcggt
     3721 gtagccatta cgcctcctgc ttatcacgca gcagctggaa ctcgcagctc tgcggaatag
     3781 tcaggtggca gccaatattc atcgcccagg cttcaacctt acacaggaag acatacatct
     3841 ctccggtatc aaggtcggag gtatggcgta acgactggat agtggtgata tcaccggtta
     3901 cgacatcaac caggtctttg gtttcataac cgagatatgt gtgtttgaga gcatctttta
     3961 cccaagctgg agtggcgaac gttttacccc tgctgatgag gtattcactg atttcgctgt
     4021 accacatgtg gctgagtgca ttctgggaaa gactgcgtct ctcgcgccac ggtttaagca
     4081 ccatgcgaaa gcatttgccg tcctccagat aaggctggat ctgccgaccg atagcggtga
     4141 agttaccgcg atgtaatttg atgccatctt gtgggaggtt cacgcttcac ctccgcagag
     4201 gtcaaacgtt ggatgcaaag aatcgcaggt gcatttctgc atctgtgaag agagaagaga
     4261 gtttagattg tatgtgcgca taaacgtccc cgtttagcgc agaagtcacc aacagatgtt
     4321 cagtctgtcg acgt
//
LOCUS       DAOTRK010000242         4195 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.245, whole genome shotgun sequence.
ACCESSION   DAOTRK010000242 DAOTRK010000000
VERSION     DAOTRK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.245"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            365..1075
                     /locus_tag="QIE41_004563"
     CDS             365..1075
                     /locus_tag="QIE41_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HDS3533340.1"
                     /translation="MSTWVTITEAVEITTKAIKQKITPSDIYRHALSGNILLSVYFQS
                     PVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIID
                     TPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGS
                     LLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPR
                     KWRFDATNSELCAERHEC"
     gene            1002..3124
                     /locus_tag="QIE41_004564"
                     /pseudo
     CDS             1002..3124
                     /locus_tag="QIE41_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3294..>4195)
                     /locus_tag="QIE41_004565"
     CDS             complement(3294..>4195)
                     /locus_tag="QIE41_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDS3533341.1"
                     /translation="ELHKIKHLLIVTSTINLEVFNALKLEQNKLTLVTMTMQDHATLK
                     EIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCK
                     NASRMDQNLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIAL
                     NLEGSSQERTFSLSIAENLIAKIQSETDMPIVIVYGPKGEDKARVLVDCYNNVYRLSL
                     SPSIKRSAAIIKDAYMAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVAV
                     GQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1238 a          965 c          909 g         1083 t
ORIGIN      
        1 tagattatat attctcatag ctaccataaa ctatactagc ctgaactata tttattctgc
       61 tgcaatcaat gcatacataa cacaaatatc actcaggtac actactcaaa ccacgctggg
      121 atttttcctc aagttataat catcaccccg aaaatcattc ggcatttact cattaaatag
      181 tcaccccata ggcctgtaca tgtttactca gaaatataca tccttttctc tgtcataaac
      241 cctctgatta atcataaata aatacttgtg acaccaatct ttttccttaa cggaacgaat
      301 tgttgtgtag aaggagataa tattatgtca aaaaaaatat cagccattgc ttataactca
      361 ttatatgtca acatgggtca ctataacgga agcagttgag atcactacca aagccataaa
      421 acaaaaaatt actcctagcg atatttatcg tcatgccttg agtggcaaca tcctactatc
      481 ggtctatttc cagtctcctg tgatacttaa gaagatacaa acttttaatg gaaaaataaa
      541 attcaggcag tttgtgggag acctacttga taaactatgc atgcttgaca gggacggttt
      601 tatttatggt caaaatttaa gactctgtac agaagccaga tatatttgtc ctgtgcaaca
      661 aattatagat acccccctgt tacgcaaact caaccagttt cgtacgtttg ttcgtaatgt
      721 ccgcccgggt gatgaactgg atgtccaggc acaagttagt gaaaaaaatt taaccccgcc
      781 gccgggtaac agcagtggca accttgagca acagatagcc agtacttcac agctaattgg
      841 atctctgctc gccgaagata tgaacagcga gcaagcggca aacatagcac gtggatgggc
      901 ctcttctcag gcttcagggg taatgacaga ctggttaagc cgcttcggta ccgcaagaat
      961 cacgctgggc gtggatgaag atttcagcct gaagaactcc cgtgacggca accctcgaaa
     1021 atggcgattc gatgcaacaa acagtgaact atgtgccgaa cgtcacgaat gctgaaatca
     1081 cgctggcagc ctcgaaggat ccggtgattg ccgacaataa cgatctcacg acactaacag
     1141 caccgtcgct gatacagagg gcaatgcgat agccaacact gaggtaacat ttactctacc
     1201 ggaagatgtg aaggcgaact tcacgctgag cgatggcgac aaagcggtta ctgatgccga
     1261 tggcaaagcg aaagtcacgc tgaaaggtac aaaagcaggc gctcataccg tgacagcatc
     1321 gatggttggc ggtaagagtg agcagttggt ggtgaacttt actgcggata cgctcactgc
     1381 gcaggttaat cttaacgtta ccgaggacaa tttcatcgcc aataacattg gaatgaccag
     1441 gctacaggca acagtgactg atggaaacgg caacccagta gaaggtataa aagttaattt
     1501 ccgtggaacc tccgtcacgc taagcagcac cagcgttgaa acggatgatc aggttttcgc
     1561 tgaaattctt gtgacaagca ccgaggtcgg actaaaaaca gtttcagcct ctctggcaga
     1621 taaacctact gaagtcatct cgcgattact gaatgccaag gtagatgtta attctgcgac
     1681 gattaccagt caggagatac ctgaaggtca ggtcatggtc gcacaagaca tagcagttaa
     1741 agctcacgtt aacgaccagt ttggcaaccc ggtgacgcat caacccgcga cattcagtgc
     1801 agcgccatcc tcgcaaatga tcatcagcca gaatacggtc tctactaata cgcagggagt
     1861 agccgaggtc accatgacgc ctgaaagaaa cggctcgtat actgtaaaag catccctagc
     1921 gaatggagct tcacttgaga aacaactgga ggctattgat gaaaaactga cactcacgtc
     1981 cagtccgctt atcggtgtca atgctcctaa aggcgctact ctgacggcaa cgctaacctc
     2041 tgcaaatggc actccagtgg agggtcaggt catcaacttt agcgtaacgc tagaaggtgc
     2101 gacgttaagt ggcggaaaag tgagaaccaa ctcttcaggt caggctccag tcgttctgac
     2161 cagcaataaa gtcggtacat atacggtgac tgcatcgttc cataacggcg tcacaataca
     2221 gacacagaca accgtgaagg ttactggcaa cccaagcacc gcacatgttg ctagctttat
     2281 cgctgatccg tcgactatcg ccgccaccaa cagtgattta agtaccttaa aggcaacggt
     2341 tgaggatggc agtggtaacc tgatcgaagg tctcactgtg tacttcgcct taaaaagcgg
     2401 ctccaccaca ttaacgtcat taacagcagt gaccgatcaa aacggaatcg cgacaacaag
     2461 cgtgaaagga gcgataacgg ggagcgtcac tgtaagcaca gtcacaagcg caggtggaat
     2521 gcaaacagta gatatatcgc tagtggcagt cccagcagac gcctcgcagt ccatccttaa
     2581 gaacaaccag tcatcactga aaggggactt taccgatagt gctgagctac atcttgttct
     2641 gcacgatata tcaggcaatc cgatcaaagt ttctgaaggg atggaatttg tgcaatcagg
     2701 tacaaacgtg ccctatatga aaattagcgc aattgattac agtcaaaata tcaacggtga
     2761 ttacaaagct actgttacag gagacggaga gggtatcgca acgctgatcc ccgtattgaa
     2821 tggtgttcat caggcgggcc tgagtaccac gatagaattc attagcgcgg aaaccagacc
     2881 aatgaccggt acagtgtcag tgaatggtgc aaacctaccg acagcttcat tcccttcgca
     2941 gggatttacc ggggcgtatt atcagttgaa taatgacaac tttgcaccag gaaaaacggc
     3001 ggctgattat tcgttttcaa gctcggcctc ctgggtaggc gttgatgcta ctggtaaagt
     3061 gacattcaaa aatgacggtg atagcaatac tgtaataatc acagcgtccc caagatccgg
     3121 tggtgctatt taccaaacag ttcctcctga atcgcgttcg gtgtgactat gatcattttc
     3181 ggtgagtatt gctttgatcc aaaggctggt tttctgtgat gattgaccag cctcagagta
     3241 ttgatggcac caccgttcat aacagatcgg cattactatg cataagtact gcctcaaatt
     3301 ctcgccaaaa cactttttaa tgcctttgcg aattcatcca gacttatatt gtcaattttt
     3361 tgcccaacgg cgactgactc cgaaacatcg gccattgcgg gccatcgcgt tttgtaatca
     3421 gcataaattg caacaacagg ggtattatag gcgcttgcca tatgtaatat tgaggtgtca
     3481 ggagttattg ccatataagc atcttttata attgctgctg aacgtttaat cgagggtgat
     3541 aaggataaac ggtagacatt attataacaa tccactaata ccctggcttt atcttcccct
     3601 ttgggtccat aaacgatcac tattggcata tctgtttcac tttgaatttt tgctattaga
     3661 ttttctgcaa tcgataatga aaatgtacgt tcctgcgagc taccttctaa attgagcgca
     3721 atgtaagatc ctaacgatcg catttcctcg cgtacctcat cgagtacatc atcgcttaat
     3781 ggcaattcat atattgacct gactgccgga aaacccgcct cacgcatcat aaatgcccat
     3841 gtcatgggta caggagcccg gagattctga tccatccggg acgcgttctt acacaaaggg
     3901 gaatagcact tcatggttaa accgacgacc tggaaattcg tttttgcttt caactgactg
     3961 ataaatatca tcgttttcag gttcttttta cgcatcgcct ctatacaaag atccggcgta
     4021 ccgtattgca gtgttatgtt cttcgctaaa tcttttattt cttttaatgt tgcgtgatcc
     4081 tgcatagtca ttgtgactaa tgttaattta ttctgttcaa gtttgagcgc attaaagact
     4141 tctaaattaa ttgtcgacgt aacaattaat agatgcttaa ttttatgcaa ttcaa
//
LOCUS       DAOTRK010000243         4184 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.246, whole genome shotgun sequence.
ACCESSION   DAOTRK010000243 DAOTRK010000000
VERSION     DAOTRK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.246"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            219..899
                     /gene="rnc"
                     /locus_tag="QIE41_004566"
     CDS             219..899
                     /gene="rnc"
                     /locus_tag="QIE41_004566"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDS3533342.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            896..1801
                     /gene="era"
                     /locus_tag="QIE41_004567"
     CDS             896..1801
                     /gene="era"
                     /locus_tag="QIE41_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDS3533343.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            1813..2541
                     /gene="recO"
                     /locus_tag="QIE41_004568"
     CDS             1813..2541
                     /gene="recO"
                     /locus_tag="QIE41_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDS3533344.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            2553..3284
                     /gene="pdxJ"
                     /locus_tag="QIE41_004569"
     CDS             2553..3284
                     /gene="pdxJ"
                     /locus_tag="QIE41_004569"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDS3533345.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIQVSLFIDADEGQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            3284..3664
                     /locus_tag="QIE41_004570"
     CDS             3284..3664
                     /locus_tag="QIE41_004570"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDS3533346.1"
                     /translation="MAILGLGTDIVEIARIEAVITRSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            4084..4164
                     /gene="shoB"
                     /locus_tag="QIE41_004571"
     CDS             4084..4164
                     /gene="shoB"
                     /locus_tag="QIE41_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDS3533347.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         1066 a         1014 c         1124 g          980 t
ORIGIN      
        1 gagataaatc tcccgtggct aacgacatcc cccgtcgttg tgtatagaat attcccccga
       61 agtttaaggt tggcacctcc aggttgccac ggcacacgaa acagcgttgg tccccatata
      121 ccggtaaact gaaactgcag cgaagcagtt agcagaacca tgtatatcag gtctgtttcg
      181 tgtgctgaat tgttgacgca tttatttatt ggtatcgcat gaaccccatc gtaattaatc
      241 ggcttcaacg gaagctgggc tacactttta atcatcagga actgttgcag caggcattaa
      301 cccatcgtag tgccagcagc aaacataacg agcgtttaga atttttaggc gactctattc
      361 tgagctacgt tatcgccaat gcgctttatc accgtttccc tcgtgtggat gaaggcgata
      421 tgagccggat gcgcgccacg ctggtccgtg gcaatacgct ggcagaactg gcgcgcgaat
      481 ttgagttagg cgagtgctta cgtttagggc caggtgaact taaaagcggt ggatttcgtc
      541 gtgagtcaat tctcgccgac accgtcgaag cattaattgg tggcgtattc ctcgacagtg
      601 atattcaaac cgtcgagaaa ttaatcctta actggtatca aacccgtctg gacgaaatta
      661 gcccaggtga caaacaaaaa gatccgaaaa cgcgcttgca agaatatttg cagggtcgcc
      721 atctgccgct gccgacttat ctggtcgtcc aggtacgtgg cgaagcacac gatcaggaat
      781 ttactatcca ctgccaggtc agcggcctga gtgaaccggt ggttggcaca ggttcaagcc
      841 gtcgtaaggc tgagcaggct gccgccgaac aggcgttgaa aaaactggag ctggaatgag
      901 catcgataaa agttactgcg gatttattgc tatcgtcgga cgtccgaacg ttggcaaatc
      961 cacattgttg aacaaactgc tggggcagaa gatctccatt acctcccgca aggcgcaaac
     1021 aactcgtcac cgcattgtgg ggatccatac tgaaggcgcg tatcaggcga tctacgttga
     1081 taccccaggc ctgcatatgg aagaaaaacg cgccattaac cgcctgatga acaaagcggc
     1141 gagcagctct atcggcgacg ttgagctggt gatttttgtc gtagaaggca cccgctggac
     1201 gccggacgat gaaatggtgc tcaacaaact gcgcgatggc aaagcgccgg taatcctcgc
     1261 ggtgaacaaa gtggataacg tgcaggaaaa agccgatctg ctgccacact tacagttcct
     1321 ggcaagccag atgaacttcc tcgatatcgt gccgatctct gccgaaaccg ggttgaatgt
     1381 tgatactatt gcggcaatcg tgcgtaagca tctgcctgaa gcgactcatc acttcccgga
     1441 agattacatc accgatcgct cacagcgttt tatggcgtct gaaatcatcc gcgaaaaact
     1501 gatgcgtttc ctcggcgctg aactgccgta ctccgtgacc gtggagatcg aacgtttcgt
     1561 ctctaacgaa cgcggcggtt atgacatcaa cggtttgatt ctcgttgagc gtgaagggca
     1621 gaagaagatg gttattggca acaaaggggc caagatcaaa accatcggga ttgaagcgcg
     1681 taaagacatg caggaaatgt tcgaagcgcc tgttcacctt gagctgtggg taaaagtgaa
     1741 atccggttgg gccgacgacg aacgcgcact gcgcagtctc ggttacgttg acgatcttta
     1801 agagtaactc cgatggaagg ctggcagcgc gcatttgtcc tgcatagtcg cccgtggagc
     1861 gaaaccagcc tgatgctgga cgtcttcacg gaggaatcgg gtcgcgtgcg tctggttgcc
     1921 aaaggcgcac gctctaaacg ctctactctg aaaggtgcat tacagccttt cacccctctc
     1981 ttgctacgtt tcggcgggcg tggcgaagtc aaaacgctgc gcagtgctga agccgtctcg
     2041 ctggcgctgc cattaagcgg tatcacgctt tacagcggtc tgtacatcaa cgaacttctc
     2101 tcccgcgtac tggaatacga gacgcgcttc tctgaactct ttttcgatta cttgcactgc
     2161 attcagtctc ttgcaggtgc cactggtacg ccagaacccg cgctgcgccg ctttgaactg
     2221 gcactgctcg ggcatctggg ctatggcgtc aattttaccc attgtgcggg tagcggcgag
     2281 ccggtagatg acaccatgac gtatcgttat cgcgaagaaa aagggtttat cgcaagcgtc
     2341 gttatcgaca ataaaacgtt caccggaagg cagttaaaag cgttaaacgc gcgggaattt
     2401 cctgacgcag acacactgcg cgccgcgaaa cgctttaccc gcatggcgct taagccgtat
     2461 cttggcggta aaccattaaa gagcagggaa ctgttccggc agtttatgcc taagcgaacg
     2521 gtgaaaacac attatgaatg atgaggattg tcatggctga attactgtta ggcgtcaaca
     2581 ttgaccatat tgcaacgctg cgtaacgcac gcggtactgc ttacccggat ccggtgcagg
     2641 cggcgtttat tgctgagcag gcgggagcgg acggcattac cgtgcattta cgtgaagatc
     2701 gccgccacat tactgaccgt gatgtgcgca tcctgcgtca gacgctggat acccgcatga
     2761 atctggagat ggcggtgacc gaagagatgc tggcgatcgc cgttgagacg aagccacatt
     2821 tttgctgtct ggtaccggaa aagcgtcagg aagtaacaac cgaaggcggc ctggacgtcg
     2881 cagggcagcg tgacaaaatg cgcgatgcct gtaaacgtct ggcggaggcc gggatccagg
     2941 tttctctgtt tatcgacgcc gatgaagggc agatcaaagc tgcggcagag gttggcgcac
     3001 cgtttatcga gatccacacc ggttgctatg ctgatgccaa aactgacgcc gaacaggcgc
     3061 aagagctggc gcgtatcgcc aaagccgcga cctttgccgc tagtctcggt ctgaaagtta
     3121 acgctggtca cggtctgacc tatcacaacg tgaaagctat tgccgccatc cctgagatgc
     3181 atgaactgaa tatcggtcat gccattattg gtcgtgcagt gatgaccgga ctgaaagatg
     3241 cggtggcaga aatgaagcgt ttgatgctgg aagcgcgtgg ctaatggcaa tattaggttt
     3301 aggtacggat attgtggaga tcgctcgcat tgaagcggtg atcacccgat ccggtgagcg
     3361 cctggcacgc cgcgtattaa gcgataacga atgggaaatt tggaaaacgc accaccagcc
     3421 ggtgcgtttt ctggcgaaac gctttgccgt aaaagaagcc gcagcaaaag cgtttggcac
     3481 cgggatccgc aatggtctgg cgtttaatca gtttgaagta tttaacgatg agcttggtaa
     3541 gccacggctg cgactgtggg gcgaggcatt aaaactggcg gaaaagctcg gcgttgtaaa
     3601 tatgcatgta acgctggcag atgaacgcca ctatgcttgc gccacggtaa ttattgaaag
     3661 ttaaaataat accgttctct ttaaaaagta aaacccccga actggtaagg aacagggggt
     3721 ttactttaca actttaattt tgtgcagcac gcaccctcaa caagagcgat tatggatcag
     3781 ggcggttatc ttttacaagc aacttgcaat ctttagcata aaaactcgag cctttacgaa
     3841 gaaaacaata ttgatggaaa gattaacgtg accgccaatt cggaagtaca ttaaaattgg
     3901 cttcgttatt ggcgattttg ctgtgcttta taccatgcca cagaattccc ccattgaaac
     3961 gagtggtgtc gtcaaagctc tggtgtggag tgcagcatgc accctcaata actcgcacgt
     4021 tcagttttgg ggagatgtaa gggctaatct gaacggctgc attccttgtt taaggaaaaa
     4081 cgaatgactg attgccgata ccttattaaa cgggtcatca aaatcatcat tgctgtttta
     4141 cagctgatcc ttctgttctt ataacataag gaaacgtact taag
//
LOCUS       DAOTRK010000244         3941 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.247, whole genome shotgun sequence.
ACCESSION   DAOTRK010000244 DAOTRK010000000
VERSION     DAOTRK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.247"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            110..229
                     /locus_tag="QIE41_004572"
                     /pseudo
     CDS             110..229
                     /locus_tag="QIE41_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(442..1299)
                     /gene="sitD"
                     /locus_tag="QIE41_004573"
     CDS             complement(442..1299)
                     /gene="sitD"
                     /locus_tag="QIE41_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDS3533348.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGVTIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRCLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLAIGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNT
                     HTQDLSSPN"
     gene            complement(1296..2153)
                     /gene="sitC"
                     /locus_tag="QIE41_004574"
     CDS             complement(1296..2153)
                     /gene="sitC"
                     /locus_tag="QIE41_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDS3533349.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2150..2977)
                     /gene="sitB"
                     /locus_tag="QIE41_004575"
     CDS             complement(2150..2977)
                     /gene="sitB"
                     /locus_tag="QIE41_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDS3533350.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDEHPFVAHRPSAVQGEAR"
     gene            complement(2977..3891)
                     /gene="sitA"
                     /locus_tag="QIE41_004576"
     CDS             complement(2977..3891)
                     /gene="sitA"
                     /locus_tag="QIE41_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDS3533351.1"
                     /translation="MYSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDMVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1043 a         1007 c          969 g          922 t
ORIGIN      
        1 acacgtgcat gggatgtggc aagcctgatg gattttgctg tgccggtggt ggcgtgtctg
       61 ctggtctggc tgttggttaa tcgtgggtga aaaaaggtga gctgtatatg caacggagga
      121 agaaaccccg ttgctggaag cctggaaaaa gtatcggatg ctgctgaacc gtgttgatac
      181 atcaactgca cctgatattg agtggcctac gaaccctgtc agggagtaat cattgggatt
      241 atgccgcaga aacgttgtat gcaataacgt tctgcggttg gctgatgcac tttcgagagt
      301 gcatgtattg aattatttcc agctattatc gattttacgt attttttgca tgagagaatt
      361 tgtacctcct cccaacaacg accttccatg actttccgga cttctgtggg ccagcggacg
      421 atgctgccct cggatgttgt gctaattggg tgatgacaaa tcctgtgtat gggtgtttcc
      481 ctttgtgcgt gcgttgatgc tggtaacggt gaacgtcatg atgaacacaa ccgcgaataa
      541 gacgacaatc gtcggagctg gtgcactgtc gataaaaaag gacaaccaga caccagtcat
      601 tgacactatt accgatatgc caatcgccag taggagcgca atatgaaagc gttgtgtcag
      661 gagtaccgca atggcgccag gcgcaatgag cagagaaatg gagagaatga tacctaccgc
      721 tttcagcgtc gccacaatgg tcagagagac catgcacagc agcccataat gcagacatct
      781 cgtatgcaaa ccgcttacct gcgcctgttg ataatcaaag ctgaacagca aaaaatcccg
      841 ccattttacg ctaataacaa gtgtaaccag cccggcaata atcaccgtct ggattatatc
      901 gccgatggtt accccgagca tgtcgccgaa aagaatatgg tcaagatgta cgtctggttt
      961 gactgcaata tacaagatca gacctgcggc aaacatgcca gaaaaaacaa tccccatcac
     1021 tgtatcttgc ttgattcggc tgttgtcttt caggtatccc gtcgccaccg cgcagaatac
     1081 tccggcgacg aatgcccctg tagccagagg taaccccaga atccaggcaa gcacgatgcc
     1141 aggaaaaacc gcatgactca tcgcgtcacc catcagtgcc caacctttca ggaccagaaa
     1201 gaccgacagc agcgcacagg gtatggaaac taccagtgaa atcagcagtg cgtgactcat
     1261 aaaggtgaac tgcagtggct ccagtaataa tgccatcatg agcgatcctc cagcgcttta
     1321 tgcgcacgcc gacgatttgc caaaagccca tgggtgggcg caaatacaaa tgctaaaaga
     1381 aacagcagtg tctgcgccac cacgatgatt ccacctgtcg cgccgtccag aaagtaactg
     1441 acccacgcgc cgaggaaact ggttacactg ccaatagtta cagcaattat gagcaggcgg
     1501 ggaaaacgat cggttaacaa ccatgctgtt gcaccgggag tcaccaccag acagatcacc
     1561 agaaaggcac caaccgtttg cagcgccgca acggtcgaaa ccgacagcag ggtgaagaag
     1621 aggattttta atctgcccgg atgcaatcca atagcacgag cgtgattctc gtcgaaaaag
     1681 gtcaccataa gatctttcca tttgaaaaaa agcacgatga tcgagagaat gccaatgata
     1741 gtgagttgca ggatatccgc cggatcgatt gccagaatat tgcccaggac gatggtctga
     1801 atgttcacgg atgtcgggtt caatgacacc ataaacagcc ccaggccaaa aaaggaggag
     1861 aagatcaggc cgatgatggc atcttctttt aagcgagtgc gttggttaag aaacagcatg
     1921 ctgcccgctg ccagtccgcc ggaaaagaag gctcccagag aaaacggaag ccccagcata
     1981 tatgcccccg caacacccgg gacaatggag tgcgacagcg catcgccaat cagcgaccag
     2041 ccttttagca tcagatagca tgacaggaaa gcgcagagac cgccgaccat cgccgagacc
     2101 cacatcgcat tgagcatata ttcatagctg aagggttcca gtagcacgtt catcttgctt
     2161 ccccctgcac cgctgacggt cgatgtgcca caaaagggtg ttcatcgtca gtaataatgc
     2221 tttcttccga gccgttaagt gtgacatggc gcagtacgcc gctaaaagcc agctccaggt
     2281 tctcggctgt aaaagtggtg tctgtcggcc cgcttgccag gacggtgcct ttgaccatga
     2341 ccgtataatc gcaaaatgtc gtgactgagc cgagattgtg cgttgagaca agcatcgtct
     2401 tgccttccgc gcgtaactcc ctcaacaggc tgatgatttt cgcttcggtt ttcacatcaa
     2461 cgccggtaaa aggttcatcc agcaggatca catcaccctg ctgtgcaatc gctctcgcca
     2521 gaaagacgcg tttcttttgt ccaccggaaa gctcgccgat ttgtcgatgg cgaaaatcca
     2581 ccatatcgac acgttccagc gcatcggtga caatctgacg atccctcttt ttggctatac
     2641 gcaacatacc catgtgccca tagcggccca tcatcaccac atcttccact agtacaggaa
     2701 atgaccagtc aacctcttct gactgaggga cgtaggcaac caggtttttc tgtagcgcct
     2761 gtcgcgtggg aatacccaga acagatatct ttccgctggt cagacgcaca aatcccataa
     2821 tcgctttaaa caacgtcgat tttccggaac cgtttacccc aaccagagcg gcaatagatc
     2881 cacttggtac tgtgaaggat gcatcgcgca gtgctgtgtg cccgttacgc caggtgacag
     2941 taacatcatt aacgacaatg cccgcagatt gcatcattat ttctcccttt tcccggcttt
     3001 aattccctgt accagcgtac tggtagtaac cttcagaagg tcgatatacg taggtaccgg
     3061 gccgttttct gtgctcaggg aatcgacata gagtacaccg ccgtagtgcg cgccggtttc
     3121 acgcgcaacc tgacgcgctg gtttatcgga aatcgtactc tcgctaaaga ctgccgggat
     3181 atgatttttc ttcaccatat caacaacctt acgtacctgc tgcggcgtgc cttgttgatc
     3241 ggcattaatc ggccacagat aaagctcttt tagccccaaa tcgcgtgcga gataagaaaa
     3301 agccccttca ctggtgacca tccatcgctg attctcaggg agttcagcaa tctgcttacg
     3361 caggggggca agggtttggg taatcttggc tttataagta tcggcattac gttggtaggt
     3421 ttgtgcattt gccggatcgt attttatcaa cgcatcacga atattatcga cgtaaatcag
     3481 agcattatct ggcgacatcc aggcatgggg gttaggtttg ccctcatagg ggccttcagt
     3541 gatccctact ggcgtcacac ccgaagagac aattacttct ggaaccccat taagatgctg
     3601 gtaaaagcgt tggaaccaca attccagatt cataccattg gcgagaatca gttgtgcccc
     3661 ctgcgcacgt ttaatatcgc caggggtagg ctgatactca tgaatttctg caccgggctt
     3721 ggttatggat gagacttctg cagcatctcc agccacgttt ttggccatat ctgcgatgat
     3781 ggtaaatgtt gtaatgacct tgaatttttc agttgcactc gcctgaaatg cgatcgagca
     3841 ggtgagggcg aggcacccca agagcatggt tacttttttt attgagtaca taatttagta
     3901 cctatagtga gcaaaattag aacaaacata gcaacggcta t
//
LOCUS       DAOTRK010000245         3928 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.248, whole genome shotgun sequence.
ACCESSION   DAOTRK010000245 DAOTRK010000000
VERSION     DAOTRK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.248"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(144..488)
                     /locus_tag="QIE41_004577"
     CDS             complement(144..488)
                     /locus_tag="QIE41_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDS3533352.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            620..2530
                     /locus_tag="QIE41_004578"
     CDS             620..2530
                     /locus_tag="QIE41_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDS3533353.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSTQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2588..3283
                     /gene="tsaB"
                     /locus_tag="QIE41_004579"
     CDS             2588..3283
                     /gene="tsaB"
                     /locus_tag="QIE41_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDS3533354.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3322..3903
                     /gene="yeaY"
                     /locus_tag="QIE41_004580"
     CDS             3322..3903
                     /gene="yeaY"
                     /locus_tag="QIE41_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDS3533355.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
BASE COUNT          933 a          993 c         1086 g          916 t
ORIGIN      
        1 ttatagataa aattgaaatt ataaaaataa ataatgaatt agcagaagca tcaacagtaa
       61 tatcttattc tctggtcgat ttatgtaaaa ccgaccagta ttatcgataa attatcgtct
      121 gacttatttt gcttcgataa agcttacacc gcagccacaa tcttaatttc aactttatac
      181 ttcgggttca tcaaacccgc ttgtaccgtg cagcgcaccg gcgcatgacc cgcgacaacc
      241 caggcatccc acgctttatt catcgccgcg aagtcgtttt tatcggccag gaaaatggtg
      301 gcatccagaa tgctcgattt attgctgccc tgtttttcca gcacggcgtc aatctgcgcc
      361 agcgtgttgg cggtttgctc aaaggcatcg gcgtcgaggt tttccggtac accagtgtag
      421 tagagcgtgt tgttgtggat tactacatca gaccagcggg cttcagcatc gatacgaacg
      481 atagtcatca ttttgtcctc attatccttc cattttcgtg ctgcaaagac tgccacaatg
      541 tgaggtcgcc gtcaccactt caactggcga aagcgccctc atcctgacat aatccctgtt
      601 caaatcaaca gggggtagtg tgacggacga ttttgcacca gacggtcagc tggcgaaagc
      661 gataccaggc tttaagccgc gagaaccaca gcgacagatg gcggtagccg tcacccaggc
      721 gatagaaaaa ggccagccgc tggtggtgga agcaggaacc ggtacgggca aaacctacgc
      781 ttacctggct cctgcgctgc gggcgaaaaa gaaagtcatt atctcgaccg gctcaaaagc
      841 gttgcaggat cagctctaca gccgcgattt gccaacagtc tcaaaggcgt tgaaatatac
      901 gggcaacgtg gcgttgctga aagggcgctc aaactacctc tgcctcgaac gtctcgaaca
      961 gcaggcgctg gcggggggcg atctgccggt acaaatctta agcgatgtga tcctgctgcg
     1021 ctcctggtct aatcaaacag tcgatggtga tatcagcacc tgcgtcagcg tggcggaaga
     1081 ttcacaggcg tggccgctgg tcaccagcac caacgataac tgccttggca gcgactgccc
     1141 gatgtataaa gattgctttg tggtcaaagc acgcaaaaaa gcgatggacg ccgatgtggt
     1201 ggtggtaaac catcatctct ttctggcgga tatggtggtg aaagagagtg gatttggcga
     1261 actgatcccg gaagctgacg tcatgatctt cgacgaggcc caccaactgc ccgacattgc
     1321 cagccagtat tttggtcagt cactctccag tcgacaactg ctcgacctgg caaaagacat
     1381 caccatcgcc tatcgcaccg aattaaaaga tactcagcag ttacaaaagt gcgccgaccg
     1441 ccttgcccag agcacgcagg attttcgtct gcaactcggt gagcctggtt atcgcggtaa
     1501 cctgcgtgag ctgttagcta atccgcaaat tcagcgggca tttttactgc tcgatgacac
     1561 cctggaactt tgttatgacg tggcgaaact ttcgctgggg cgttccgcct tgctggatgc
     1621 ggcatttgaa cgcgccacgt tatatcgcac gcgactcaaa cggctaaaag agattaatca
     1681 gccgggttac agctactggt acgaatgtac ctcgcgccat ttcactctcg cgctgacgcc
     1741 gcttagcgtg gcggataaat tcaaagagtt aatggcacaa aagcccggta gctggatctt
     1801 tacctcagca acgctgtcgg tgaacgacga tctgcatcat ttcacctcgc ggcttggcat
     1861 tgaacaggca gagtcgttgc tgttacccag cccgtttgat tacagccgcc aggcgttact
     1921 ctgtgtgccg cgcaacctgc cgcaaaccaa tcaaccgggt tgtgcacggc aactggcggc
     1981 aatgctgcga ccgatcatcg aggctaacaa cggtcgttgt tttatgcttt gtacctcgca
     2041 cgccatgatg cgcgatctgg ccgagcagtt ccgcgctacc atgacgcttc ccgttttgtt
     2101 gcagggggaa accagcaaag ggcaactgtt gcagcaattt gtcagcgccg gtaacgcgct
     2161 tcttgtggca accagcagct tctgggaagg ggtggatgtg cgtggcgata cattgtcatt
     2221 ggtgattatc gacaagttgc cgtttacctc gccggatgat ccactgttaa aagcgcgcat
     2281 ggaagattgc cgtttgcgtg gtggtgaccc gttcgatgaa gtacagctgc cggatgcggt
     2341 cattactctc aagcagggag tagggcgatt gattcgcgac gccgacgatc gaggagtttt
     2401 ggtgatttgc gataatcggc tggtgatgcg cccttacggc gcgacgtttc tcgccagtct
     2461 gccgccagcg ccgcgcaccc gtgacattgc ccgtgcggtt cgtttccttg cgataccatc
     2521 ctccaggtaa tttgtgatgg agtatggtat gatgcgcgcc tttttattga tctaagcacg
     2581 aagacccatg cgaattctgg ctatcgatac cgcgacagag gcctgctctg tcgccctgtg
     2641 gaacgacggt actgtcaacg ctcattttga gctttgccct cgtgaacata ctcaacgaat
     2701 tttaccgatg gtgcaggata tcctgaccac cagcggaact tccttgactg acataaatgc
     2761 tctggcatac gggcgcggcc ccggtagctt taccggcgtg cgcattggta ttggcattgc
     2821 gcaagggctg gcacttggcg cggaattacc gatgattggc gtctccacgc taatgacaat
     2881 ggcgcaaggg gcgtggcgca aaaacggcgc aacccgcgtg ctggctgcta ttgatgcgcg
     2941 gatgggcgaa gtttactggg ccgaatatca gcgtgatgaa aacggtatct ggcacggtga
     3001 agaaaccgaa gccgtactca aacccgaact cgttcatgaa cggatgcaac agctttccgg
     3061 ggaatgggtg acggtaggta caggctggca agcctggccg gatctcggta aagagagcgg
     3121 gctggttttg cgcgacggag aagttttact gccagcggcg gaagatatgc ttccgattgc
     3181 gtgtcagatg tttgccgagg gtaaaacggt ggcggtggaa catgccgaac cggtttattt
     3241 acgtaacaac gtcgcatgga agaaacttcc gggcaaagaa tgaatcttaa gagtatgccc
     3301 tgagaaaaaa ggagtcgcat catggcggtt caaaagaatg ttatcaaagg catactggca
     3361 ggtacgtttg cgctaatgct gagcggttgt gttactgtgc cggacgccat taaaggcagc
     3421 agtcccacgc cgcaacaaga tttagttcgg gtgatgagtg cgccgcagct gtacgttggt
     3481 caggaggctc gctttggtgg caaagtggtt gcggtacaaa accagcaagg gaaaacccgc
     3541 ctggaaattg ccaccgtacc actggacagc ggagccagac cgacgctggg agaaccttca
     3601 cgcggtcgca tttatgctga tgtgaacggt tttctggacc cggtagattt ccgtggacaa
     3661 ctggttacgg tagtcgggcc aatcaccggt gcggttgacg gcaaaatcgg caacacgccc
     3721 tataaattta tggtgatgca ggtaacgggt tacaaacgtt ggcatttaac ccagcaggtg
     3781 attatgccgc ctcagccgat tgatccatgg ttttatggcg gtcgtggctg gccctatggc
     3841 tacggcggat ggggctggta taatcccggc cccgcgagag tacaaacagt tgtaactgaa
     3901 taattgcttg tttttaaaga aaaagaaa
//
LOCUS       DAOTRK010000246         3892 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.249, whole genome shotgun sequence.
ACCESSION   DAOTRK010000246 DAOTRK010000000
VERSION     DAOTRK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.249"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..312)
                     /locus_tag="QIE41_004581"
     CDS             complement(<1..312)
                     /locus_tag="QIE41_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3533356.1"
                     /translation="MVSFMRLRFPRSVEAWLGWVIVLMLVFFSIMSDEFLSIQNLLDL
                     SESYAVTGIFALGLFVVLVTGGIDISFAAVASVVQYVVASLLLNETLSNPFFCLVFAV
                     FF"
     gene            complement(305..1819)
                     /locus_tag="QIE41_004582"
     CDS             complement(305..1819)
                     /locus_tag="QIE41_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDS3533357.1"
                     /translation="MPHIIEKNAEQPLVTLRSLTKSFGGHRALKNINLTLNKGEVHCL
                     AGANGCGKSTLIKTLCGVYVPDSGSEIIIDGKSWTRLSPDKARELGIQVIYQDLSLFP
                     NLSVAENIAFEYNLKGYLGLHRKKQLRDVSQKIIQELEFNLDPDALVQHLPIAQRQQV
                     AICRALIADARLVIMDEPTASLTRTEVNQLLRTVNYLRDKNITVVFVSHRLDEVKEIS
                     DRITVIRDGEIIGTWAANQLSPGDITELMTGKTIVHQKKLPNSVEGKVVLELKKLCRK
                     GQFEDVSLKLHQGEVLGLCGLLGAGRTELALSLFGITRADSGDIYLEGKPVTIKDNTH
                     AVKLGIAYVSEDRLTLGAVLPQSISDNMVLSILDRLKTPSGFIDENRKETLVKEWIQD
                     LDIKVTDPNNPLSTLSGGNQQKVVLAKWILTRPKVLILDAPTVGVDIGAKDSIYQLIH
                     RLSGVGISVILITDEAPEAYYNCDRIFHMKRGKIIHELLPSNYTEQQLAEVING"
     gene            complement(1886..2890)
                     /locus_tag="QIE41_004583"
     CDS             complement(1886..2890)
                     /locus_tag="QIE41_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDS3533358.1"
                     /translation="MKKLILPCLIATVLSSTAAWADQTVTSQKANKPFTMGVVVKVGG
                     IPWFNVMEQGIKDEGKLLGVDAWQVGPTTADPAEQVRAIEDLIARKVDVIGVVPNDAK
                     VLEPVLKRAQEAGIKVITHESPDQKNADWDFELLNTQTMGANHMKDMAKCMGEEGKYA
                     MFVGSLTVPLVNDWANAAIAYQKEHYPNMTLVEDRFGVAESVDDSMRTTNDLMSKYKD
                     LKGIMSFGSQGPIGAGRAIDKRKKNDAICVFGTFTPGQGIKLLEKGAIDGGYISNPMI
                     AGKVFVQVATAMMNGEPIKDGVKIGDMGEIKVNNNTIYSDNPEKLDLENTRHLVKLGL
                     "
     gene            complement(3013..3822)
                     /locus_tag="QIE41_004584"
     CDS             complement(3013..3822)
                     /locus_tag="QIE41_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDS3533359.1"
                     /translation="MLQAERHKMICAHVQKQGAVRVTELSRLFKASPETIRRDLTVLE
                     RNKKLIRSFGGAVALDSSDSAPVMLADNAWQDNSIDRAESFRKRTEENPDIKARIAKA
                     ALQFIHPGDCILMDNSSTCWFLARQIPDIDVTVVTNSLRIIQALACRDRVRIIGIGGE
                     YSERHDDFHGPVAEGAIRNFQINSFFFSCQGLNLGNGVRDGSEINARLKQVMLQVSGQ
                     KILLVDSSKFEQYAFSKICTLDEIDILVTNVCTDTRYRTQFPQLNIVEVDK"
BASE COUNT         1151 a          920 c          681 g         1140 t
ORIGIN      
        1 aaaaaaaaca gcaaacacca ggcagaaaaa tggatttgat agcgtttcat taagcagtaa
       61 cgacgccaca acatattgta caaccgaagc tactgctgca aaagagatat caattccgcc
      121 agtaaccaat acgacaaata atccgagggc gaaaataccc gttacggcat aactttccga
      181 gagatcgagc aagttctgta ttgataaaaa ctcatcgctc atgatggaaa aaaataccag
      241 catgagaaca atgacccatc ctaaccaagc ttcgactgaa cgaggaaacc gcagccgcat
      301 aaagctaacc attaatcacc tccgccaact gctgctcggt ataattcgaa ggcaacagct
      361 catgaataat ctttccgcgc ttcatatgaa aaatacgatc gcagttataa taagcttctg
      421 gtgcttcatc agtaattaat atcactgaaa tacccacacc tgaaagccga tgaataagct
      481 ggtaaatact atcttttgcg ccaatatcta cacctaccgt tggcgcatcc agaattaaaa
      541 ccttaggtcg tgttaaaatc catttagcca ggacaacctt ttgttgattg ccaccagata
      601 gcgttgaaag tggattattt ggatcggtga ccttaatatc caaatcctgg atccactcct
      661 tgactaaggt ttcttttcga ttttcatcaa taaaccccga tggcgtcttt aaacgatcca
      721 gaatcgagag caccatatta tcgctgatag attggggtaa tacggccccc agtgtcaggc
      781 gatcttctga aacatatgct attcccagtt ttaccgcatg cgtgttgtct ttaattgtaa
      841 cgggcttacc ttcaagataa atatcaccac tatctgctcg agtaataccg aaaagactca
      901 atgctaattc ggttctcccc gcaccaagta aaccacacaa tcccagaacc tcgccctggt
      961 gtagttttag agatacatct tcaaattgcc cttttctgca taattttttt agttcaagaa
     1021 ctactttacc ttcaacgcta tttggtagtt ttttctggtg gactatagtt tttccggtca
     1081 ttaactcagt aatatcgcct ggagaaagtt gattggcagc ccacgttcca attatctctc
     1141 catcacggat gaccgtaatt cgatctgaga tttccttaac ttcatcaagg cggtggctaa
     1201 caaaaactac ggtaatattt ttatcgcgta agtaattcac tgtgcgtagt aattgattaa
     1261 cttcagtgcg cgtcaatgac gccgttggct cgtccatgat gactaaccgg gcatcggcaa
     1321 tcagagcccg gcaaatagcg acctgttggc gttgtgcaat cggcaaatgt tgtactaacg
     1381 catcaggatc gagattgaat tccagctcct gaattatttt ctgtgataca tcccgaagtt
     1441 gtttttttct gtgcagccct aaatatccct tcaggttgta ttcaaaagcg atattttcag
     1501 ccacagaaag atttgggaat aaagaaagat cctgatatat cacctgaata cccagttccc
     1561 gagctttatc aggagaaaga cgagtccagg acttaccatc tataataatt tcacttcccg
     1621 aatcaggaac ataaacacca cacagcgttt tgatcagagt acttttacca cacccattcg
     1681 caccagcaag acaatgaacc tcacctttat ttaaggtaag attgatattt tttagagccc
     1741 tatggccacc gaaacttttc gttagtgacc ttagcgtcac taatggttgc tccgcattct
     1801 tctctataat atgaggcatc ataatattcc tgaatacgac taaaggaatt ccgggtcaaa
     1861 tttgacccgg tgatttacgg taaaattaca agcctaactt cactaaatgt cgtgtatttt
     1921 caagatcgag cttttctgga ttatcactat aaatagtgtt gttattaacc ttgatctcgc
     1981 ccatatcacc gatttttaca ccgtctttaa taggttcacc gttcatcatt gccgtcgcaa
     2041 cctggacaaa aaccttaccc gcaatcatag ggtttgaaat atatccccca tcaatagcgc
     2101 ctttttcgag tagcttaatt ccctgacctg gagtgaaggt tccaaaaacg caaattgcgt
     2161 catttttctt ccgcttatca atagcacgcc ccgcaccaat tggtccttgt gaaccaaatg
     2221 acataatgcc tttaagatct ttatatttag acattaaatc gttcgtcgtg cgcattgaat
     2281 catccaccga ttcagcaaca ccaaaacgat cttcaaccaa cgtcatattt ggataatgtt
     2341 ctttttgata agcaatcgcg gcattggccc agtcatttac caaaggcacg gtcagactgc
     2401 cgacaaacat ggcgtattta ccttcttcgc ccatgcactt cgccatatct ttcatatgat
     2461 tggcacccat tgtctgggta tttaacaact caaaatccca atctgcattt ttttgatctg
     2521 gtgattcgtg ggtaataact ttaataccgg cttcttgtgc acgcttaagt acaggttcaa
     2581 gaacctttgc atcgttagga acaaccccaa taacatccac ttttcgtgca attaaatctt
     2641 caatcgcccg aacctgttca gctggatctg ccgttgtagg gccgacctgc catgcatcaa
     2701 cccccaatag ttttccttca tctttaatgc cttgttccat cacattaaac cagggaatgc
     2761 caccgacttt tactactacc cccatcgtaa aaggtttatt cgctttttgt gatgttacag
     2821 tctgatcagc ccatgcagca gtggaagata atacggtagc aataaggcag ggtaagatta
     2881 attttttcat cgtaatttcc ttaagaaata gttcaggtgt caggaaaact cgatataata
     2941 tcgacctgac tatttatata atttataatc ccttaggcat tgattaataa tttagacaat
     3001 aaataatgcc tattatttat caacctccac aatgtttaat tgtggaaatt gtgttcgata
     3061 ccgagtatct gtacaaacat tagtcaccag aatatcgatt tcatccagcg tgcatatttt
     3121 actaaatgca tactgttcaa acttactgga gtcgaccagc aatatttttt gtcctgacac
     3181 ctgaagcatc acctgtttaa gtctggcatt aatttcactg ccatcgcgga caccattccc
     3241 cagattcaac ccctggcatg aaaagaaaaa actgttgatt tgaaaatttc ggatcgcccc
     3301 ctctgcaacc gggccatgaa aatcatcatg tcgctcagaa tactcaccac cgataccaat
     3361 aattcgaact cgatcacggc aagccaacgc ctgaattatc cgtaatgaat ttgtaactac
     3421 agtaacatct atatcgggaa tttgacgagc taaaaaccaa caagtactac tgttatccat
     3481 taaaatacaa tcaccagggt gaataaattg caatgcggcc ttcgcaattc tggcttttat
     3541 atcaggattt tcttcagtcc gtttacggaa tgattccgca cgatcgatac tattatcttg
     3601 ccacgcatta tcggcgagca taaccggagc gctatcagat gaatccagag cgaccgcgcc
     3661 accaaagcta cggattaact ttttgttgcg ctccagtacc gttaaatccc gacgaattgt
     3721 ctcaggagac gctttaaata atcgtgataa ttctgttacc cgtacagcac cttgtttttg
     3781 tacatgtgcg catatcattt tatggcgctc tgcctgcaac atagtatcgg tccgttatta
     3841 tcaaattagc atgcttatat aattaactgt tccctcataa aataaagtga ta
//
LOCUS       DAOTRK010000247         3884 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.250, whole genome shotgun sequence.
ACCESSION   DAOTRK010000247 DAOTRK010000000
VERSION     DAOTRK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.250"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..92)
                     /gene="ariR"
                     /locus_tag="QIE41_004585"
     CDS             complement(<1..92)
                     /gene="ariR"
                     /locus_tag="QIE41_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HDS3533360.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEES"
     gene            complement(121..393)
                     /locus_tag="QIE41_004586"
     CDS             complement(121..393)
                     /locus_tag="QIE41_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDS3533361.1"
                     /translation="MKTSDNESTKYEITGQAVLHILRMKINFSLQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGEPNRRTAIADNKSMFNGKKINRIH"
     gene            complement(436..672)
                     /locus_tag="QIE41_004587"
     CDS             complement(436..672)
                     /locus_tag="QIE41_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDS3533362.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            986..2197
                     /gene="bluF"
                     /locus_tag="QIE41_004588"
     CDS             986..2197
                     /gene="bluF"
                     /locus_tag="QIE41_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDS3533363.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQAAYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            2402..3133
                     /gene="bluR"
                     /locus_tag="QIE41_004589"
     CDS             2402..3133
                     /gene="bluR"
                     /locus_tag="QIE41_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDS3533364.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIEAPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3354..3758
                     /locus_tag="QIE41_004590"
     CDS             3354..3758
                     /locus_tag="QIE41_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDS3533365.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHYRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT         1153 a          800 c          783 g         1148 t
ORIGIN      
        1 gactcttctt ctaacaaatt acctgaacta cgaaagtagc ttgataacgc tttatcagtt
       61 attgcattat gaattgtagt atcttcaagc atggtaacct ctcatcttac ttatgaaatt
      121 ttaatgtatt ctgtttattt tcttaccatt gaacatactt ttattgtcag ctatcgctgt
      181 tcttctgttt ggctctcctg tatcactgtt gctaaaatca cgaattatgc tgtcaattaa
      241 atctcgtcgg aaagcatttt cctcagccga tttcattacg agtaattgct taataagagt
      301 ctgtaacgaa aaatttatct tcatgcgcag tatatggagc accgcctggc cagtaatttc
      361 atattttgta ctttcattat cagatgtctt cataacgact tcatagctaa cctgttaaat
      421 aatcaggctg ttgcattatt caaaaagcaa cccaattagt gcattataat gtttctgttc
      481 ctcttcccct gtcgcttgtt ccagtcggca tagaagtttg gcacaaagcg actttcgact
      541 cagattacgt ccatctttca aaatctcagt cacaatttcc ccgagagttt cctgctgagt
      601 aggcaagtta gcttttgcaa agtaacgagt gattgctccc gcagaatcta aagtcactga
      661 attttgatgc atgctacgcc tctgttaaaa atgttaatca atgttaagtg aggtaacaat
      721 agcaaacttg tacgaaatat gtgtacagaa tgataaatta attttttgca tttagtgcta
      781 atacatataa aacaacaggt tatgataaaa aatattgcat catttaaatg acaacctata
      841 caagaatttt cgccaactca tcttcaactt tttccatttt ttatcaatgt aaagaaacag
      901 ttatgttttg gtattaaaag aaagacaccg gaagatatgt ctgttaccgt cttactctcg
      961 cctctcccat taccctggac agttaatgct taccaccctt atttatcgta gccatatacg
     1021 tgacgacgaa cctgtcaaaa aaatcgaaga aatggtttcg atagcaaatc gcaggaacat
     1081 gcagtctgac gtaacaggga tcttactgtt taatggatct cattttttcc agcttctgga
     1141 aggtccggaa gaacaggtta aaatgatata tcgggctata tgccaggatc cacggcacta
     1201 taatattgtt gagctgctgt gcgattacgc gcctgctcgc cgttttggca aagcgggaat
     1261 ggaattattt gatttgcgcc tgcacgagcg agatgacgtt ttacaggccg tattcgacaa
     1321 aggcacatca aaatttcagc taacttatga tgacagagcg ctacaatttt ttcgtacttt
     1381 tgtccttgca accgaacaag cagcctattt cgagatccct gccgaagact cctggctttt
     1441 tatcgctgac ggatctgata aagaacttga ttcctgtacc ctttcaccaa ctataaacga
     1501 ccactttgcc tttcatccta ttgtcgatcc cttatcgcgg cggataatcg cttttgaagc
     1561 cattgtgcaa aaaaatgaag acagcccgtc agctatagcg gttgggcagc gtaaagaagg
     1621 ggaaatctat acagcggatc tcaaaagtaa ggcgcttgca ttcgcgatgg cacacgcact
     1681 tgacctcggt gataaaatga tttcaatcaa tctattacct atgaccctgg ttaacgaacc
     1741 tgacgcagtc tcttttttac ttgatgaaat aaaggccaat gctctggtgc ctgaacaaat
     1801 catcgttgaa tttactgaaa gtgaagtcat atctcggttt gatgagtttg cagaagcgat
     1861 taaatcgctt aaggctgccg gtatcagtgt agcaattgat cattttggcg caggttttgc
     1921 cggtttgtta ctcctgtcac gcttccagcc tgacagaatt aaaatcagtc aggaattgat
     1981 taccaatgtt cataaaagcg ggccacggca ggcaattatt caagcgatca taaaatgctg
     2041 tacatcactt gaaattcaag tcagtgctat gggcgtggca acaccagaag agtggatgtg
     2101 gcttgaatcc gcaggaattg agatgtttca gggagatctg tttgcgaaag ctaaattgaa
     2161 tggtatccct tcagttgcgt ggccagagaa aaaataattt tcagcatatt atttcacatg
     2221 atttcagtaa atcatcgtga aaatgaatac gttatgttta aatttaatcc acattaaaaa
     2281 taatgtttca ttcaatttag ctgaaatctt atttatactt tgtacaattt tagcgtatat
     2341 tgtacaaagt attattgggt cgtgtacagg cgacggagat ttgtgaccgc aaggaggaat
     2401 tgtggcttat tacagcattg gtgatgttgc tgaacgttgc gggattaatc ctgtcactct
     2461 ccgggcctgg caacgccgct acggtttgtt aaaaccacag cgcagtgaag gcggacaccg
     2521 actctttgat gaagaagaca tacaacgcat cgaagagatc aagcgttgga taagtaatgg
     2581 cgtccctgta ggcaaagtta aagcattact ggaaaccacc agccaggata cggaagatga
     2641 ctggagccgc ctgcaagaag agatgatgtc aattcttcgt atggctaatc ctgccaaact
     2701 acgcgcgaga attatttcac tgggtcgaga gtacccagta gatcaattga ttaatcatgt
     2761 ttaccttcct gttcgccagc gtctcgtgct tgatcacaac acctcccgca ttatgagcag
     2821 tatgtttgac ggcgcattaa ttgaatacgc agcagcctcg cttttcgaaa tgcgccgtaa
     2881 gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa gagagagcac gattgtggct
     2941 ggaagcatgg cgtttatcat tgtcaggatg gcacatttct gttcttgctg atcccattga
     3001 agcgccgcgc ccggaactgt tcccaacgca aacattgatt gtctggacag gcatggcacc
     3061 aacgagaagg caaaacgaac ttttacaaca ctggggtgag caaggttata aagtcatctt
     3121 tcatgccccc taatttcacc tttatcccgc cccttagaca gaatcttata atctgaaccc
     3181 acaacagcgt ttgtaccgga gctggtccgt tgcaaaatgc tcaccgggag aattcaggta
     3241 atcccccttt cacactttgt acatatcaca gccaaaggac agaccagatt gaagtggtaa
     3301 aaccgatcgt gcacactacg cagtctcaca actaacaaga ggttattatc ctgatggatc
     3361 aggttgttgt ttttcaaaag atgtttgagc aagtgagaaa agagcaaaac ttctcctggt
     3421 tttattcaga attaaaacat taccgtattg cacattacat ttattatctg gctacggata
     3481 acatcagaat tattactcac gatgacacgg ttttgttatt aagaggaacc aggaacctgt
     3541 taaaagttag tacgaccaag aaccctgcta aaataaaaga ggccgcattg cttcatattt
     3601 gcggaaaatc aacatttcgg gaatactgtt caacactggc agacgcgggc gttttccggt
     3661 gggttactga tgttaatcat aacaaacgca gttactacgc cattgataat acgcttttat
     3721 atattgaaga tgtagaaaat aataaaccat taatctagct aaagttggat gcttaaaaaa
     3781 tgcttcataa ttcagtaagg cattagcata atggaaataa aagtgcagag actattcata
     3841 tggatgagta atactatctt tttattgcca cccataaata atca
//
LOCUS       DAOTRK010000248         3820 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.251, whole genome shotgun sequence.
ACCESSION   DAOTRK010000248 DAOTRK010000000
VERSION     DAOTRK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.251"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1308)
                     /locus_tag="QIE41_004591"
     CDS             complement(<1..1308)
                     /locus_tag="QIE41_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDS3533366.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYATQLLDDYSILSYITDEESPIE
                     IKDNNSISNGEYLTTKDESHAVKVDDGVTGYINNASVMTSGDGSYGISVDSHNKVLYI
                     SDSDIKTSGSVSDKGNNQDVNGGITASAVVSEFGGTIVMNGDNSVETRGAYSAGLLSQ
                     VNDSGIVENNTRLETTDKTNIVTYGENAVGVLACSSPGESRTCVDAVDDEVSDSNSYE
                     VISRADLKMNGGSITTNGTNSYGAYANGEKAYINLDYVALETGEHGSYAVAIRQGNID
                     IKNSSITTKGTKAPIAKIYNGGELFFSNVTAVSEQDKGISIDASNIDSQAKIALSSVE
                     LSSALDSIDVNKTTTDVSILNRSIITPGNNILVNNTGGGLNIISSDSTLNGATKLVSG
                     TTTLKLSENTIWNMKDDSVVIHLTNSDSIINLSYDDGQTFTQGK"
     gene            complement(1708..1926)
                     /locus_tag="QIE41_004592"
     CDS             complement(1708..1926)
                     /locus_tag="QIE41_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533367.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITILVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVSHRDQQ"
     gene            complement(2058..3581)
                     /locus_tag="QIE41_004593"
     CDS             complement(2058..3581)
                     /locus_tag="QIE41_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDS3533368.1"
                     /translation="MRNTLIPIFIAICLFITGVTILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         1147 a          831 c          719 g         1123 t
ORIGIN      
        1 ttttccctgg gtaaatgttt gaccatcatc atacgaaaga ttgataatac tgtctgaatt
       61 agtcagatgg ataacaacgg agtcatcttt catattccag attgtgtttt ctgaaagctt
      121 cagtgtagtt gtgccgctaa cgagtttagt cgctccattt agagtagagt cggacgaaat
      181 tatgtttaag ccacctccag tattattaac cagaatatta ttaccaggcg tgataatact
      241 tcgattaagg atgcttacat ccgttgtagt tttgttaaca tcaatactat ccaaagcact
      301 tgaaagttcg acacttgata gtgctatttt ggcttgagaa tcgatatttg atgcatcaat
      361 tgatattcct ttatcttgtt ctgataccgc ggtgacattg gaaaaaaata actctccacc
      421 attgtatatt tttgcaatgg gggctttagt gccttttgtt gtaatagaac tattttttat
      481 atcaatgtta ccttgtcgaa tagcaactgc ataacttcca tgctccccgg tttcaagtgc
      541 cacataatct aaattaatat atgctttttc cccattagca taagcaccat agctattagt
      601 gccattagtt gttatggaac caccattcat ttttaaatca gcacggctaa taacttcgta
      661 actgttagaa tcactaactt catcatctac agcatcgaca catgttcgag actctccagg
      721 acttgaacat gcaagaacac ctactgcatt ttccccatag gtaacaatgt tcgttttatc
      781 tgtggtttca agacgggtgt tattttccac aatcccagaa tcattaacct gacttaaaag
      841 tcccgctgaa tatgcccccc tcgtctcgac tgaattatca ccattcataa cgatggtgcc
      901 accaaattca ctgactactg cgctggctgt tatccctcca ttaacatctt ggttatttcc
      961 tttgtcagat acgcttccag aggtcttaat atcgctatcg cttatatata atactttgtt
     1021 atgactatca acagaaatac cataagatcc atcaccacta gtcatcacac tggcattatt
     1081 tatatatcca gttacaccgt catccacttt cacagcatgg ctttcgtctt tagtggttag
     1141 atactctcca ttacttatcg aattattatc tttgatttca atcggcgatt cttcatcagt
     1201 tatataggaa agtatactat aatcatccaa caattgtgtt gcatatgcat tacccaccgc
     1261 caaagcaata agtcctaaac taaacccggg gagttttttc agtttcatag attaaccatc
     1321 cattcattgg attaacaaac attcctggtc aaataaccta tgagttcaga ttaccaaaac
     1381 ggagggtaaa gacaaaaaca aaagcacaaa aaatttaaat agataaacag cgacaatata
     1441 taaatgataa ataaggcggc attatcattt ttcaccttat tttcatgaca ttgatcactt
     1501 tggggtgacg attttgttta acaaataact cttcgacatc aaaattatta atagttgcca
     1561 tagaaattat ttcgatgata tataaaacca actgtgataa caccagggtt gacaactatt
     1621 tgcgcaataa ttcctttcct tcaatgatcc aggcgttcac tgtcagcacc ccattcagcc
     1681 gcatactgac agtgagaaac tgaaagatta ttgctgatcc ctgtggctga cgagtctttc
     1741 ccttaatcga ttaatcagat tgatcgccag ccaaacaagg gcattaagca aatttatcgt
     1801 cacaagcact tctcccagcc cggtactggg ttttgcaata aggatgacca ggatagtgat
     1861 cagcataaat gcaatggaaa agatgatata gagagtgaaa taatccagat tattactatt
     1921 gttcatgcta ctaatggcaa tcctgtcaaa actttcaaac aattatagca aacaaaataa
     1981 tgaacaatag taacaagagg caacgagcta attagctgtc tataattaca ccgcactatg
     2041 gccttactga tcgattttta ctcaaccaca accttcacct tcggtttaga aaggataatt
     2101 ttgaccaggt cgatgtatgt taccggttta tagaaataat aaccctgctg aaatgtgata
     2161 ttattttgat tcagatagtc aagttgttct ttcgtttcga caccttcagc gacgatactg
     2221 atcgaaagtt tacgcgccag atccaataca caatccagaa tccgagttga ttctgggtct
     2281 gcgtttacgc ggccaacgaa actgtgatca attttgatat aatcaatatg caggtcatgc
     2341 agataagaaa gccctgagta gccagtaccg aaatcatcca gcgcgatgac aaaaccattt
     2401 tcatgcagaa tgttcaaccg ctgaaccaga ctttcatcaa catttaatgg ctcacgctcg
     2461 gtaacttcca gaacgaggtt taaatcgcga cgggtaaaac tgtcacggta atttaagcac
     2521 tcgtcgacaa atgtcggcga aataatatgc gaggcgctaa aattaatccc aatatgaaaa
     2581 ccttccggca gtttactcgc gatagcgttc atctgtctgg caacctgatt catcaggctt
     2641 tgcgtaagcg gaacgattaa tccggatttt tcagcaagtg gaataaatgc cgcgggtgat
     2701 atatatccac cgtgaggttg tttccagcgg gctaacacct caactccccg caatgtccct
     2761 tcccgaccat ttaccacagg ttggtaaaat gggacgatct ccccattatt tatagcccgt
     2821 cgcaggatct cttcaggcgt tgcgctttta ttgaaatacc tatcaagcag gaaggcagcg
     2881 gcgcaggcga ttaacagaat aaatatcaaa atcccaaagc ctttatcgat aagtctgtag
     2941 aagctaaaga gtggcggcgg gttaaagata atagtgaaat gatattttgt ggagtgaacc
     3001 ttctcggtat agggatagtg tccattaagc gtcatcacat cacccgtagg tccaataatg
     3061 tcatctgcca cgcgtaatac ataccttacc cctttcaagg gtacattaag cgccccgcga
     3121 atatgctgat cacttatcgt aaccaaaatg cgcgtatctg caaattggct ctgatagagc
     3181 aggataggta atctattaac ggtgtcgatt gccggagcca gcagtaaatt actgtccggg
     3241 aaaacaggaa tacgagacag taggacccga ttccccggta aggatgtaca ccagaccgtt
     3301 ccctgtttga gaatgatgat tgtgcgcagg tgaggtttca gagctgcttc agtgccaagc
     3361 tgatattgct cctcgaggtc gcattccttc ccggcaatgt taaccgccgt ttgagtcgcc
     3421 tgtgacgctt cttcaagtat atgattgata ttgttggctg catatcttgc tcccgccagg
     3481 tactctgctt ttgcggaata ccagagctgg atgttcaaaa tagtgacacc ggttataaac
     3541 aagcatatcg caatgaagat gggtatgagt gtattgcgca tttgcttgtc ccggccaaag
     3601 ggtttaatac atgaaaatgc cattcattcg ctcgcactgg catctacaga gagcatggta
     3661 gagagcataa gaaatatcca cgccgaagat aaaattttct ccatcttcat ctaacctatt
     3721 aattattgag cacaatgtag tcagaattaa acaaattagg tgggatatca ggcgtcaaga
     3781 atggaaaacg agcgctctcc attcctgttt gccccctgat
//
LOCUS       DAOTRK010000249         3783 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.252, whole genome shotgun sequence.
ACCESSION   DAOTRK010000249 DAOTRK010000000
VERSION     DAOTRK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.252"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(85..303)
                     /gene="osmB"
                     /locus_tag="QIE41_004594"
     CDS             complement(85..303)
                     /gene="osmB"
                     /locus_tag="QIE41_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDS3533369.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(572..1321)
                     /locus_tag="QIE41_004595"
     CDS             complement(572..1321)
                     /locus_tag="QIE41_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDS3533370.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(1411..1584)
                     /locus_tag="QIE41_004596"
     CDS             complement(1411..1584)
                     /locus_tag="QIE41_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533371.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(1731..3716)
                     /gene="pdeR"
                     /locus_tag="QIE41_004597"
     CDS             complement(1731..3716)
                     /gene="pdeR"
                     /locus_tag="QIE41_004597"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDS3533372.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESKKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         1034 a          914 c          854 g          981 t
ORIGIN      
        1 actgtgtgta ttattcagtt atttcgtact gaacagaatc aagcgtatta taattgcgag
       61 tcgataatcg acatttattt acgattattt accgacctgg tgaccaataa caccaccgac
      121 ggcagcacca cctaatgtac ccagcgtact gccatcggtc agtactgcac cgcctaatgc
      181 acctgcaccc gcgccaattg cggtgttacg gtctcgttta gaccagttag aacaggcact
      241 cagagacatt gccagggtaa ttgccagaac agccgcggtc atttttttgc tcgttacaaa
      301 cataatactc tctcctgaat ttatgattca cggaagtaag ctcactataa ctataatagc
      361 taagaataag cctggtgaaa ttattccgtg aaatccgctc gcgcgcaggg ttatatcacg
      421 caggtgataa tgacttcctg ttatatcgct gataataatt ttatatcttg agagtgttaa
      481 taacaggtaa atagtcttaa ttatcaacca ggaatcatct tagagcggat gatttgcaaa
      541 acagcaaatc atccgtagag aagggaaatg gttaaatgtc aacgacgtga acggtgagtt
      601 tactgtgctc aagatgcatc agatcgctgg cgcgtatttt cgaatcggta atcacgcgat
      661 tgaatcgctc aacggggccg atggagtatg aatgtacagc accaaatttc gagctgtcag
      721 tcaggactat cgcttcgcac tctttttcca gcacggcatt gaccacatcg gtacgcatca
      781 tatcgcgacc ggtaaaccca gtttcaggtt gccagccatc aataccaata aatgctttgc
      841 tgaaatgcac ctgttggatg cactggcgtg tcaaaggacc aaccatactt tcgctttttt
      901 tctggtacac gccaccgagc aaaataactt cacaaggcgc gtctttcagc aaatgcgcga
      961 tgtagctgct gaccgtgatg atagtgacat ttttcttctg ctcgcccaga gtccgggcga
     1021 gcagggcatt gctgctgcca ttttcgataa agatggtttc gcccggttga accagtgacg
     1081 cggcaaactc ggcgagttca cgcttcagcg tatagttgct catcatacga gtttcgacgt
     1141 catcactatc aagcgatact gcaaagccat gtgcacggcg gaggtaactc agtttttcga
     1201 gggtgttgag atcctggcga atggtaactt cagaaactcc agtggctttt gccagatcgg
     1261 ttacgctaac ctgaccctgg tcaatgacca tctgtagaat tgtttgttgt cgggaattca
     1321 tcgctgtaat tcttataacg ttataatact aaataaaaaa atactgacgc gccgggcatc
     1381 ctcaaatagg aaaacctgaa ggcacgattg ttaaatctcc cacggtgcct tcgggatgtc
     1441 tggcgtagat tcagcaacct ggcgcaagag ttctgcttta agtatctcga gattatggat
     1501 cgcagagtgg taatcgcctg ccacaaggat atctaacaca atatcaatac gttcggcaac
     1561 gcgctgggca tcaaattcag acatcaagga catccttata gcaaaagtgg aaaaggaata
     1621 atgcaaaaat tgccgctaaa agagaagtgt ttaacagcaa cggctaatta tcatccagta
     1681 atacgatata taatgacgga atatatcgct aagtatatat attcatctac ttatgcgcgc
     1741 ttcagatagc gtttatacca acgttcgaag gcgacggcgg gcatcggttt ggcaaacaaa
     1801 aatccttgcc gctcattgat cccgttcttg gttaaaaaag catcttcctt tttactctcc
     1861 acaccttcag cgatcacctg aagattcaat gcctgggcca cagcgacgat cgcccggact
     1921 agcgactgcg atacaggttg tttgtgaata tctcgaacaa aaacctggtc aagtttaatc
     1981 gcatcgatcg gaaagcgcgc cagttgcgaa agtgaagagt agccggtacc aaaatcgtcc
     2041 agatgcactt gcgcacctag ttggctaaat tgttgaataa cagaaagtgc cagttcatca
     2101 ttctcaatca ggcagctctc cgtcagttca acatcaatag ggcagtattc aaaattgagt
     2161 tcctggagaa cctgtttcag ggcggtgaaa atggtttgat cggcgagctg acgtgcagaa
     2221 atgtttaccg ccactcgcag gttaatacct ttatcccgcc actttgccac ctggcgtacg
     2281 acatcgagaa tcacccaacg gcctaaaggc acaatgagcc ctgactcttc ggcgtaggaa
     2341 atgaagtcca acggtggaat caacccacgt tcaggtgact gccaacgtac tagagcttcc
     2401 agactgcgca cttcgccacg ccaggtgatt ttcggttgat agtgaataac caactgatcg
     2461 ttttccagtg ctttacgcaa gttggtatcc agccagagat attcaaatac ccgctgattc
     2521 atttctggag taaaaacgca aaattgtcct cgtccgcctt ccttcgctgt gtacattgct
     2581 gtgtcggcgt gacgaataat agccgcgctg tccgaaccat gttcgggaga gagtgaaata
     2641 cccactgaac agctggtata aacttcaatt aaaccaatgc ggaaggggag tcgtaagcga
     2701 gtcaaaattc gtgatgccat tgcttccagc gcgctttgtg aggtattgga tgccagtacc
     2761 agaaactcat cgccacctgg acgcgccaaa acctggtcat gctccagaca gcttaaaatg
     2821 gccaatgaca cgtcgcgtaa tagctggtca ccaaacaaat gcccataggc gtcgttgacc
     2881 tttttaaaat tatccaaatc aagatacaca accccaactt tattgttatc tgcctgatta
     2941 atagcgtgat cgattaactc ctgcattgcg ttacgattcg gcagtccggt gatactgtcg
     3001 gtatttgcca gaatgcgcag tcgctcctga gcgcggcgct cttcggtaat gtcggtgccg
     3061 gaacagatta aaaaaatctc gtttttgcca ctgccgctgt ggacaaattt attgcgaaac
     3121 agaaacagcc gctggccttt acgtgttggt atccacagtt cgacttcata tgcattgccg
     3181 cttcgaaaaa atacacggtt attgcgcctg gatgccgcag cttcacgacg gctcataaac
     3241 agtttaaaca cgctttgccc aatgacgtcg tgttctttca accctgtgta atcttcacat
     3301 aaccgattga agcgctggat attcccccga ctgtcgagaa tcactattgc ggaatgtgct
     3361 tcagaaacta cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc aaccgccggt
     3421 gtgtcatgcc atgccgacgc gttgccagcc cattcccgtt tattgatttt tcgtcctacc
     3481 agatgcacag aaagatcgct ttcatcgacg gtcagactca tcatcagact ggaagtgatg
     3541 actgtcattt ccctgatgcg agcagcctgt tcggcagata actgcaacgt acgttcaggt
     3601 tctgtggttt cggtggtaga aaagcgcaaa acatcgctgc tttccgttaa ccgccagtat
     3661 ggattgtgcg atccgagaaa gttgtacaac gttgtggact ccctaacggt tttcatgatg
     3721 catatcctcc ggttaacagc ggagtccgcg gttaaataaa aggaacaaca tgacttacat
     3781 gaa
//
LOCUS       DAOTRK010000250         3733 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.253, whole genome shotgun sequence.
ACCESSION   DAOTRK010000250 DAOTRK010000000
VERSION     DAOTRK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.253"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1241
                     /gene="sctN"
                     /locus_tag="QIE41_004598"
     CDS             <1..1241
                     /gene="sctN"
                     /locus_tag="QIE41_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HDS3533373.1"
                     /translation="SLIEAELDDVSVGEVCEIYASWQANERIARAQVVGFRNGKTLLN
                     LIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMERFVPDTPGERGNLRLI
                     DELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCGKTVLMHMLVNNTE
                     ADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVDRCNAALMATTVAE
                     YFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDSLPRLLERPGPTL
                     KGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHYPAIDVLKSVSRVF
                     GQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNAENDFAMNARPKLTNWLKQ
                     SVNEKMPMSETLKELERIVK"
     gene            1231..1689
                     /locus_tag="QIE41_004599"
     CDS             1231..1689
                     /locus_tag="QIE41_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDS3533374.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDVKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            1667..2542
                     /locus_tag="QIE41_004600"
     CDS             1667..2542
                     /locus_tag="QIE41_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDS3533375.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDSQFSTLGKKNGHMESIDNKSSLQVDRNPSLSLQTNGTEKFSTKKILN
                     MTSRDQGNDSEPSNKTIQFKEKIHNGIHTEYITDQKHSNNKDREKKYRDGDKINGPQA
                     HSLDITNERRFADNRTMFTQHIEKQRNVHTLNQNDINNSANNANARENELTYQFQRWG
                     QNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTEQDERQGQRHQPHDE
                     QENEGKFENDQKDES"
     gene            2542..3489
                     /locus_tag="QIE41_004601"
     CDS             2542..3489
                     /locus_tag="QIE41_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HDS3533376.1"
                     /translation="MFGLRKVNGNTHSFETTFQNWKEKGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLRELFPLISAELAGMALMPETERLIGEWLSLSSIPLNLKYPELKYNRL
                     CVGKVFDGVLSPAQPLIRIWTGELNIWLDKVTVCQYENAPTLDKKSLYWPIHFVIGFS
                     KTCYRTIVDIEVGDVLLISNNFAYAVIYNTKICDLIYPEELKMVDHFEYEEDFETDDF
                     DIKKSEIEINDENDYQQINSFEDLPVKIEFVLGKKIMNLYEIDDLCAKRIISLLPEAE
                     KNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK"
     gene            3479..>3733
                     /locus_tag="QIE41_004602"
     CDS             3479..>3733
                     /locus_tag="QIE41_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDS3533377.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNV"
BASE COUNT         1274 a          626 c          836 g          997 t
ORIGIN      
        1 gctcgctgat tgaagcagag cttgacgacg tttcagtggg cgaagtatgc gaaatttatg
       61 cgagctggca agcaaatgaa cgtatagcca gggctcaggt cgttggcttt cgcaatggaa
      121 agacattatt aaaccttata ggcagctctg tggggcttac acgtactgca gttttgaaac
      181 caacgggaga gcagttaacc atacagatca gtgatgcctt tcttggttca gttttgaatg
      241 cttctggtca aattatggaa aggtttgtcc cggacactcc aggcgaaagg ggaaatttac
      301 gcctgattga tgaactgcca ccgtcgtatc aggaacgtcg agttattaat acaccactag
      361 agacacggat aagagtcatt gatggcgtac tgacatgtgg cattggtcaa cgtgttggga
      421 ttttcgcatc ggctggctgt ggcaaaacgg ttttaatgca tatgcttgta aacaacactg
      481 aagctgatgt gtttgttatt ggcttgattg gcgaacgtgg tagggaagtt acggaatgcg
      541 cggaatcgct gaaaaaatca gtaaatgcag caaaatgtgt tttggtttat gccacctctg
      601 atttttcgtc agtagatcgg tgcaatgccg ccctgatggc gacaaccgtg gcagagtatt
      661 ttagagatcg aggaaagaga gttgtacttt ttatcgactc aatgacgcga tatgcacgag
      721 cactacgcga tatgaagctg gcagcgggtg aaccacctgc cagacgtggt tatcctgctt
      781 ctgtttttga cagtttacct cgcttactgg agaggccggg accaacacta aaaggaagta
      841 ttactgcgtt ttataccgta ttactggagg gggaggatga atcagaccca ctgggtgatg
      901 aaattcgctc aatccttgat ggccatatct atctaagtcg caaactcgct gggcaaggtc
      961 attatccggc aatagatgta ctaaaaagtg tgagtcgagt atttggtcag gtcactgatg
     1021 aaaaacacag agataatgct gcgcgggtaa ggaagatcct cacaactttg gaagatctac
     1081 aggtgtttat tgatttaggg gaatatcgcg ctgggcaaaa tgctgaaaat gattttgcta
     1141 tgaatgcacg gcctaaactc acaaattggt tgaaacagtc agttaatgaa aaaatgccaa
     1201 tgagtgaaac gctaaaagag cttgaacgaa ttgttaagta aagttaatcg acttatcaga
     1261 agaacagcgc agagcctggc tgcatgtgaa gcatcattac aaaagttaaa tgcagaaaaa
     1321 gaaaagttag ccgaaaagga acgcctgtat gatatgcaac ttaaaaacct acagtcactg
     1381 ttagatgtga aagagttact cggcgaagtt gtatttcgac aggatatatt ttactcgtta
     1441 cgtaaagttg cagtcattca gcagcagatc gctgagataa atcttgaaaa gcaaaaaata
     1501 gcagagcggc gaaaaatact taacaaggaa atagttcaac agcaagcaca aagaaaacac
     1561 tggtggctaa aaggagaaaa gtacgatcga ctaaagaagc gcatcaaaaa acaactttta
     1621 aatcagatgc tttatcaaga cgagcttgaa caggaggaga aatataatgg acgaagtcaa
     1681 gaaaattgaa cactgtaaaa ttttaaacac agacacaccc gataattctg cttcagattt
     1741 agacagtttc ttaaagaaaa acaaaaaaaa taaagaaatg agtgagattg tcgctggaac
     1801 cattctccag ttattacgtc agactcagga tagccaattt tctacgttgg ggaaaaaaaa
     1861 tgggcacatg gagtctattg acaacaaatc atctctccag gttgacagga atccatctct
     1921 ttctttgcaa acaaatggaa cagagaaatt ttctacaaag aaaattctca atatgacttc
     1981 acgagatcag gggaatgatt ctgaaccatc taataaaacg atacaattta aggaaaaaat
     2041 ccataatggt atccatacag aatacatcac agatcaaaaa cacagtaata ataaagatcg
     2101 tgaaaaaaaa taccgcgatg gagacaaaat aaatggaccg caggcccatt cattagacat
     2161 tactaacgaa agaaggtttg ctgataacag aaccatgttt actcaacata tcgaaaagca
     2221 aaggaatgtt catacgctca atcagaatga tataaataat tctgctaata atgccaatgc
     2281 gcgcgaaaat gaattgacat atcagtttca gcgttgggga caaaatcata cagtccggat
     2341 tctggaaagt agtgaaggga tacgactaaa accatcagac acacttgtct ccgacagact
     2401 ccatgaagcc caacacaatg atgttacagc acaacgctgg gtattgactg agcaagatga
     2461 acgacaaggg caacgacatc agcctcatga tgagcaagaa aatgaaggta aattcgaaaa
     2521 cgatcagaag gacgaaagtt aatgttcggt ctgagaaaag taaatggaaa cactcattcc
     2581 tttgaaacca cttttcaaaa ttggaaagaa aagggcgaag atgttgcgtt attaatgccc
     2641 gaatttagcg ccaaatggct accgatagca gaagagagcg gcagctggtc tggatgggtg
     2701 ttacttcgag agttatttcc cttaatttct gccgagctcg ctgggatggc tttaatgcca
     2761 gaaacggaaa ggttaatagg ggaatggctc agtttatcta gtatcccgtt aaatctaaag
     2821 tatcctgaac taaaatataa tcgtttgtgt gtcggtaaag tatttgatgg agtattgagt
     2881 ccagctcaac cactgataag aatatggaca ggggaattaa atatttggtt agataaagtc
     2941 acagtctgcc aatacgaaaa cgctccaacg ctagacaaaa aatcattata ttggcccatt
     3001 cattttgtaa ttggatttag taaaacatgt tatagaacca ttgttgatat tgaagttggt
     3061 gatgttttat taatttcaaa taattttgct tatgcggtta tttataatac aaagatttgt
     3121 gatttaattt atccagagga gttaaaaatg gttgatcatt ttgagtacga ggaagatttt
     3181 gaaacagatg actttgatat caaaaaaagc gaaatcgaga ttaatgatga aaatgactat
     3241 cagcagatta atagttttga agacttgcct gtaaaaattg agtttgttct tggaaagaaa
     3301 ataatgaatc tatatgaaat agatgatctc tgtgcaaaaa gaattatatc tttgcttcct
     3361 gaagcggaga aaaatataga aatacgtgta aatggcgcgc taactggcta tggcgaactt
     3421 gttgaagtgg atgataaatt gggcgtagaa attcattctt ggttatcagg gaataataat
     3481 gtcaaatagc atttcgctga tagcgatatt atcattattt acactattac cgtttataat
     3541 agcatccggg acatgtttta ttaaattctc gattgttttt gttattgtac gaaacgcttt
     3601 gggacttcag caagtgccat caaatatgac acttaatggg gtagctttgc tattgtcaat
     3661 gttcgtaatg atgccggttg ggaaggaaat atataataac agtcagaatg agaatctttc
     3721 attcaataat gtt
//
LOCUS       DAOTRK010000251         3720 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.254, whole genome shotgun sequence.
ACCESSION   DAOTRK010000251 DAOTRK010000000
VERSION     DAOTRK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.254"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..375)
                     /locus_tag="QIE41_004603"
     CDS             complement(<1..375)
                     /locus_tag="QIE41_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533378.1"
                     /translation="MKIVFEVNGKKVPLKSLKYISKKDQLEVMKNWFFENFEDPANAC
                     PYESREGGYAYIYGGPYDASEELQSIFGQYVKSEYIEELVDELQTQCFDWSGNSNNID
                     DWYDDDIYDAVTSSGNPYLKFID"
     gene            complement(567..989)
                     /locus_tag="QIE41_004604"
     CDS             complement(567..989)
                     /locus_tag="QIE41_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDS3533379.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEVKTGATRYLMS
                     VLARELVASGDVCNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRQNIICRECRKSEVMQRILSFYQGDVRYLLK"
     gene            complement(1030..2100)
                     /locus_tag="QIE41_004605"
     CDS             complement(1030..2100)
                     /locus_tag="QIE41_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HDS3533380.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGRPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNLGQPAGTTPEADSAYALKADSGAVQQVMTARPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            complement(2172..2597)
                     /locus_tag="QIE41_004606"
     CDS             complement(2172..2597)
                     /locus_tag="QIE41_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDS3533381.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(2581..2823)
                     /locus_tag="QIE41_004607"
     CDS             complement(2581..2823)
                     /locus_tag="QIE41_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533382.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            3215..3553
                     /locus_tag="QIE41_004608"
     CDS             3215..3553
                     /locus_tag="QIE41_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S24"
                     /protein_id="HDS3533383.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQNI"
BASE COUNT          924 a         1003 c          754 g         1039 t
ORIGIN      
        1 atcaatgaat tttaaatacg gattaccgga agacgttaca gcgtcgtaga tatcatcatc
       61 gtaccaatca tctatattat ttgagtttcc agaccagtca aaacattgtg tttgtaattc
      121 atctactaat tcctcaatat actcagattt aacatattga ccgaagatgg attgtagttc
      181 ttcgctcgca tcatacggcc cgccatatat gtaagcatag ccaccttctc tactttcata
      241 cggacaagca tttgccggat cttcaaagtt ttcaaagaac cagtttttca ttacttcgag
      301 ttgatctttt ttgcttatgt attttaaaga ttttagtgga actttctttc cattaacctc
      361 gaaaacaatt ttcacgatgt aacgctccct tgtatcaatt gcgcaactat tataccacag
      421 cgaaatacag ggcataacct gcctccttaa actaacatac catgagccag caatgtccgc
      481 tattggcaca aagcggacaa ccacgctagc tctaccctgt gccacaaaat gtcaatttgc
      541 atctgaacta atgcacttta atctcgtcac ttcaataaat accgaacatc accctgataa
      601 aacgacaata tgcgctgcat aacttcactc ttccggcact cgcgacagat tatgttctga
      661 cgcctgtcgt agcgacgtat ttctccgtca ggtaatgacc agataaggtc cggatcaacc
      721 gcagatggtt tcttcagctt tgcccttgag agctttttac gggcattttg ccagtcctta
      781 cgcgcctgtt cagacgggaa taacccgtaa ccagagttgc atacatcgcc gctggcaacc
      841 agctctcttg cgagaacgct catcagatat cttgtcgcac ctgtcttgac ttccagttgc
      901 cgtaacgtct cacgcccact ctggcgtacg agttcaacaa cctgtccttt aattttttcc
      961 cgctcttctt gtgtaaaaac ttttgccaca agccctcctg aaaattacct catgaccaga
     1021 aattaacact taccccctga agcccggcgg aatttcgtta tccggttcag aaatatgatt
     1081 cacacaacgc tggttgttcg tgccgcttac cgggagcaac caggggttct caaaattccg
     1141 gtccggtcca aaaaacgtcg tcgctcgctg aacaaattcc gttcccgttt tcccggtagc
     1201 cgccaagtat cttgcgtaac gcctcacgcc atccagcatg gcctctggtg gcaccccctc
     1261 gcgtaatctg gccttccagg cactgaaagc ggatttcttc gggtttgccc cagcacgcaa
     1321 cgggtactcc cgccagacct gttcgaacac atccggataa tccactcgtc ccacagactg
     1381 cccggtgttt tccgggacta cccgatcggc ttcccgctga atggcggaat cggcttcggg
     1441 ctgctgcagt tggtgtgatt gctccggcct tgcggtcatc acctgctgca cagcgcccga
     1501 atcggctttc agcgcatacg ctgaatcggc ttccggtgtc gtgcctgctg gctgaccaag
     1561 attgacggtc tgaacatccc ctgcctggtt cgtggcgttt tttacgccat ggaccatagt
     1621 gttttgatct tcttgatctg tatctttatc tgtatcttta tctgtcgtga ctcgtcgtga
     1681 catgtgcgtg acatttcgtg acgcgccgtg acaatcgcca ttttgttccc gctttctttc
     1741 cctctctcgc tgcgccctct tgcgctctgc aggagatttt gcggtttgcg aaatattgcc
     1801 gttgtcctct ttaagcacct ggcgtttttc ccatccagtg attaaatcac catcaagtac
     1861 ccgcccctgc atcgtctgca aaattgaatc aattacctct tctgtcacgt cgagcgcact
     1921 tgccaaatct tctgtcgtga catcaatgtg acctcgcgtg acatttcgtg acgcgctcac
     1981 caggaggtgg atatacactg ccatcactgt tgcaattggc cgccctgaca ccctggcaat
     2041 tgttcgccac ttagggtcat ttggcatgtc atgccataat ctgagccagg cgttagccat
     2101 actcacctct tctgataccg aatcttttta ctcacgagtt gccggaagcg attcgatatg
     2161 gctattgtca gtcaatgtac tgccacagca tttcctgccg ggccaccacg gttcatctga
     2221 ttgaaaccgg cgattgccac tgcgacaaaa tcatcagcgt ctctcaccag tcgctcccgc
     2281 gtctccacca gctcccgaaa ataagctgaa ctgtggctgc gcattctggc caccagcaaa
     2341 ggtggcattg ccttttcgat cgctggtaac aacgcctgaa ttttttcaac tgcatcaggg
     2401 gtgtctttct ctatccagcg gaaaattttc tgggtattgc gagccagggc ttccggatgg
     2461 ctgtcgtcgt acagttcagg aaacgtcata cccaactcaa aataagcctg ggttattcca
     2521 gctgccggaa ctttttcgcc atcaggacgc gcccaggcat tcatcgccat gcggatgtgt
     2581 tcatgcttga ttttcatgaa tccccccttg gttagaaggc ggattatgat cagaaccggg
     2641 aatgacaacc gtcggtatgt gtaactcata tttgagcgcc ccggcagtga ctgcctgaat
     2701 tagcaacgcc catttccacg gaacctcttc cccccacatg ctgactgtgg tttttgacgt
     2761 tcctagagct gcggctgttt taacaactcc gccaaaatag cctaatactt ctgatttttt
     2821 catgagtcgc tccataaaac tgaacgccaa aagtttaata atcaaaacca aagaaagtca
     2881 agaaacaaaa ccatctgtgt tttaaaatca aaacatgaac aagcaaacaa tatctgaacg
     2941 cataacccaa cgtatgcatg cgctaaacct gaaaggcaaa gaccttgtca atgccactgg
     3001 cgcatcaaaa ggctccgtaa gtcaatggat gaacggtgga ggagcgccgt cctcgcgtta
     3061 cataagttca ctggcaaaaa tattgaaagt aaacgaaaat tggcttctta atggaggaga
     3121 gttaaataca ggtgattcgc ttgatctatc tttaccgccg ataaaaacgg ttccgctact
     3181 atcacttcag caggcagcaa gctggagtga ttatatgaaa aattcctcaa taacctcttg
     3241 tgtgcagctt gtcggagaaa tcccggccaa tacctttgca gttgttctag agagtgacag
     3301 tatgtcaaca tctggtgggg gagtttccat cccaaatggt tcaacagttt ttgttgatcc
     3361 cgatcgaacc gtacaaccag gaaatattgt ccttgcctta cccaaaggga ccacaacacc
     3421 tgtcattcgt aaactggaga tagaagggcc ggatattctt ttagtcccca cgaatcctcg
     3481 ctacccttca attatgctgg atgatctatc ttgcatattg ggcgtatgct ttaaaattca
     3541 acaaaatatt taaccgacct catctatttg attaactgta tgccatcgtg gtgatggctt
     3601 aacagctgcc tgcttaaaat gttttgataa aaaaacattg acctgaaaag ttcatttttc
     3661 taaacttaat tcattccctc tccccacccc acagaatgca gggcaatact tctagttacc
//
LOCUS       DAOTRK010000252         3719 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.255, whole genome shotgun sequence.
ACCESSION   DAOTRK010000252 DAOTRK010000000
VERSION     DAOTRK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.255"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(243..2060)
                     /gene="malZ"
                     /locus_tag="QIE41_004609"
     CDS             complement(243..2060)
                     /gene="malZ"
                     /locus_tag="QIE41_004609"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDS3533384.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVAMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRILVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(2216..3589)
                     /gene="proY"
                     /locus_tag="QIE41_004610"
     CDS             complement(2216..3589)
                     /gene="proY"
                     /locus_tag="QIE41_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDS3533385.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
BASE COUNT          965 a         1070 c          886 g          798 t
ORIGIN      
        1 gcaactcagt accagtaata aacggtttta ataaattcga atttgaaatt gtcatatctt
       61 tgctccacta cagggttatc ctggagcatg ttgtacataa catcatgggc aaagaatacc
      121 aaaaatcgct catatcgatc gggacaaata tttaacaaat tcagccctga cattgagcag
      181 cttttttgcg ctgcgtagtg tcagggctgg tgactaacga cgtaacaaat aaaacagatg
      241 ctttagttca tccacaccgt agccgaaatg gcaggcaaag ccagaatccc gtctgtcagt
      301 tgcccatggc cttctttgcg ttgccattgc acgacattaa gaaacggtga cgcgggtagc
      361 accacttcac aggcctcgcc acggttgatt gccaccagta tacgctgctg attcagcacg
      421 cggacaaata ccaccacgtt atcttccgca tacagcacct gacagccgcc acgacgcagc
      481 gcctgacttt tcttacgcag cgcaatcatt cgctggtaca acgcgaataa cgccgtatcc
      541 tgcttttcca cctgccaggg gaacggctta cggcaaaacg gatcgttttt accatccagt
      601 cccacttcat caccgtaata aatgcacggt acaccaggcc aggtgaacaa ccagaccacc
      661 gccagcggca ggcgcgcaat atcccgaccg agcagcgttt taaatcgcgc agtatcgtgg
      721 ctatcgagct gattaaacat acgcagttgc tgttgatgag aaagtccggc gcggtaatta
      781 tccatccagg ccatacaggt ttgggcgtca atttgctgcg gatcataaga gatgtcggta
      841 ttggcaagaa atccccacaa cgggaatgtg aagccgcgat agttcatggc ggcatcttcc
      901 acatcggcct gtaaccattg ccgtgcatcg ccaaaatgct ctccaactat atacgcttcc
      961 ggctgggttt ctttcgccgc ttcggtgatc ccggctacgt gctgcaaatt attgcgcgcc
     1021 ccacccgctt cccccagcat atgcaccaca tccagccgcc agccgtccat attccacggc
     1081 gctttcagcc agtggcggac aatactgtct tccccgcgat aaatttcatt caccagactt
     1141 tccgactgat aatccagctt cggcaagctg gcatagccca gccagtcgag cgccgtgcca
     1201 tcgtcactaa acgagtacca gtcgcgccag ggcgattcgg ggttgtgaca agcaccaccc
     1261 gtcccgcgat tgtgtctgtc aaaccaggca tgggaatcgc cactgtggtt aaacacgccg
     1321 tccagcacca gccgcattcc cagctgctgc gtattgtgtc gcaaacgcaa caacgcccca
     1381 tcaccgccaa actgcggatc gacatggcga taatcctcgg tatcgtattt atgtacgctg
     1441 ggagctttaa acaccggatt gagatacagc gccgtcacgc cgagcttttt cagatacggc
     1501 agtttttcgc tgataccgtc cagatcgccg ccatagaacg ttgatccgcc cgcctgcgcc
     1561 gtgaccggtt catcccagtc acgcaatatg atctcttgtc cggctgcgtg atggtaataa
     1621 acatgatcct gttcagcttc acgaggaaga ctacgggcga aacgatcagg gaagatctga
     1681 taaaaaatct gatccgcagc ccattgtggg ccgatatccg ggacatcgac ggcaaactgc
     1741 tccagtcgtg ctggcggcat tcggctgaag ccctgcggtg taaaccaacg ctggcgatcg
     1801 tgccacagca atttgaaact ataacgccgc cggggttgcc cgctggagag atcgatcgcc
     1861 gcccgccatg cggtaacgcc aggctgcggc tgactgcgct gcttatgcat cgctactgac
     1921 gtttcttcgt tgtcgtgttc tgtacgcagc ataatgcgct gcggtgggtc ttcgcccgtc
     1981 agccacagcg taatgagcag ttgatctttg ctttgtttaa caaatggggg caccggcagg
     2041 tgccatgcat ttaacatcat aattcccctg tgatgaaaat ctgatcacct tgccatagcc
     2101 taatgcagga tcactcatca tctgaaggat atttggggga ggaggacgga ggcggataac
     2161 tttcggaata gccggatcag gatttcctcc tgaaccggct tacagaacgc aaggattagt
     2221 ggttttcagc cagctgacga tcatggcggc gcttaaacat ccagccaatc aacagcacaa
     2281 caatccacgc gaaaccgaca tacagcgaga tacgcgtatc agggtgataa ccaatcaacc
     2341 cgataataaa gagcaggaaa atcaaaccgc cgatggtcgt tgctacccca cccggtactt
     2401 taaatttcag cgccttaact tcttctggcg gtaaacggcg acggaaggcg atttgcgaca
     2461 gcaggatcat aatccacacc cacaccgtgg cgaaggtcgc cagcgaagcg atcaccagga
     2521 agacgttttc cggcatgatg tagttcagat ataccgcaaa caatagcgcg gtagtcatca
     2581 ccagcaccgt gacccacgga ataccacgac gcgacgtttt gctgaaaatt tttggcgcgc
     2641 tgccctgctc tgccatacca tggagcatac ggcctacgcc aaatacgtca ctgttaattg
     2701 ccgaaagcga agcggtcagc acaacaaagt taaggatgct ggcggcaaag gtaatcccca
     2761 tatgctggaa cgtcagtacg aacgggctac cggcagtgcc aacctgattc cacgggtaga
     2821 tagacataat gacgaacagc gtaccgacgt agaacaccag aatacgcatc ggcacggagt
     2881 taatcgcgcg cggaatagat ttctcaggat ctttcgcttc accggcggta atcccgataa
     2941 tttcgatccc accgtaagca aacatcacca tttgcaacga catcaccata ccaagccagc
     3001 cgttactgaa gaagccgccg ttgctccaca ggttatgaat accggtcggt tgcccgccgt
     3061 tgccaatccc ccagatgatg atgccgaaac cggcgacaat catgatgatg atggtggcga
     3121 ctttaaagaa cgagaaccag aactccagct caccgaatac cttcacgctc atcaggttta
     3181 cggcgcaaat gatcagcacc acgctcagta cccaaatcca gtgcggcacc gtcgggaacc
     3241 agacacccat atagatacca aaagcggtca catcggcgat ggcgacaata aggatttcaa
     3301 agcagtaggt ccagccagta atgtaaccag ccagcgggcc gaggttttcc tgcgcataac
     3361 gcgagaaaga gctggcggcc gggttatgta ccgacatttc ccccagcgcg cgcataatga
     3421 tatacgccgc gacaccaccg ataatatagg ccaacaacac gctcggaccg gccattttga
     3481 tggcgtctgc cgaaccgtaa aacaacccgg tgccaattgc tgaacccagt gccataaagc
     3541 gtatgtggcg ggtacttagc ccacgcttta gcttgttctt actttccatc aattcctaac
     3601 ccatcaacac aataaataaa tatcctccgg catagccgga ggtttttcaa atgcgcctat
     3661 aaggctctct taccagccgc gccctaacag gcgcacacga tctgacattt gcatccaac
//
LOCUS       DAOTRK010000253         3597 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.256, whole genome shotgun sequence.
ACCESSION   DAOTRK010000253 DAOTRK010000000
VERSION     DAOTRK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.256"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(17..241)
                     /gene="cspD"
                     /locus_tag="QIE41_004611"
     CDS             complement(17..241)
                     /gene="cspD"
                     /locus_tag="QIE41_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDS3533386.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            564..884
                     /gene="clpS"
                     /locus_tag="QIE41_004612"
     CDS             564..884
                     /gene="clpS"
                     /locus_tag="QIE41_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDS3533387.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            915..3191
                     /gene="clpA"
                     /locus_tag="QIE41_004613"
     CDS             915..3191
                     /gene="clpA"
                     /locus_tag="QIE41_004613"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDS3533388.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT          926 a          809 c         1009 g          853 t
ORIGIN      
        1 caatgagaca gaagagctat gcgactgccg cttctacttc gacgggcaca ataacactgg
       61 cgtgattgcc ttttggcccc tggtggacat caaactgaac ggattgtcca gcttttagcg
      121 ttctgtaacc atccatctga atggtggaat aatgagcgaa aatatcttcg ccgccgcctt
      181 cagggcagat gaaaccaaac cctttggcat tgttgaacca cttaacagta cccttttcca
      241 tgcttcgaca tccttcgcaa atcttataca agtaagatgg aataaaccgg ggtcagagag
      301 ggggctgttc aaaacctcgc caactctaga aatacaattt agagaattag ggcgagccgt
      361 caagcatttg acaggggcca gggggcaggt atgaatcaaa aatttgaagc agttaacgct
      421 attgacagga atgtgacaga tgtcgctgat gccaacgata gatgatagtt atctatcatg
      481 tggagtagat tggtcaggca aataagctct tgtcagcggc agggcgttct gccgataacc
      541 gtaaccgaag atgataactg acaatgggta aaacgaacga ctggctggac tttgatcaac
      601 tggcggaaga aaaagttcgc gacgcgctaa aaccgccatc tatgtataaa gtgatattag
      661 tcaatgatga ttacactccg atggagtttg ttattgacgt gttacaaaaa ttcttttctt
      721 atgatgtaga acgtgcaacg caattgatgc tcgctgttca ctaccagggg aaggccattt
      781 gcggagtctt taccgccgag gttgcagaaa ccaaagtggc gatggtgaac aagtacgcga
      841 gggagaatga gcatccattg ctgtgtacgc tagaaaaagc ctgaatgcag gcataaaaat
      901 tgggggaggt gcctatgctc aatcaagaac tggaactcag tttaaatatg gctttcgcca
      961 gagcgcgcga gcaccgtcat gagtttatga ccgtcgagca cttgttactg gcgctgctca
     1021 gtaacccatc tgcccgggag gcgctggaag cgtgttctgt ggatttggtt gcgctccgtc
     1081 aggaactgga agcctttatt gaacaaacca cacccgttct gcctgccagt gaagaggagc
     1141 gcgacacaca gccgacgctg agttttcagc gtgtgctgca acgtgcggtc ttccatgtcc
     1201 agtcctccgg tcgcaatgag gttaccggtg caaacgttct ggtcgctatc tttagcgaac
     1261 aggagtcgca ggcggcatat ctgttgcgta aacatgaagt cagccgtctc gatgtggtga
     1321 actttatctc tcatggcacg cgtaaagacg agccgacaca gtcttctgat cctggcagcc
     1381 agccaaacag cgaagaacaa gctggtgggg aggaacgtat ggagaatttc acgacgaacc
     1441 tgaatcagct tgcgcgcgtg ggcggaatcg acccactgat tggtcgtgag aaggagctgg
     1501 agcgtgctat tcaggttctc tgccgtcgcc gtaaaaacaa cccgctgctg gtgggggaat
     1561 ctggtgtcgg taaaaccgcg attgcggaag gtcttgcctg gcgaattgtt cagggtgatg
     1621 tgccggaagt gatggctgac tgtacgattt actctctcga tatcggttct ctgttagcgg
     1681 gcacaaaata tcgcggcgac tttgaaaaac gttttaaagc gttgctcaag cagctggagc
     1741 aggacactaa cagcatcctg tttattgatg agatccacac cattatcggt gcgggtgcag
     1801 cgtctggtgg tcaggtcgat gcggctaacc tgatcaaacc gttgctctcc agcggtaaaa
     1861 ttcgcgtaat tggttcgaca acctatcagg agttcagcaa cattttcgag aaagaccgtg
     1921 ctttggcgcg tcgcttccag aaaattgata ttactgaacc gtcgatcgaa gaaactgttc
     1981 aaatcatcaa cggcctgaaa ccgaagtatg aagcgcacca cgatgtgcgt tataccgcaa
     2041 aagcggtgcg tgcagcggta gagctggcgg tgaaatacat taacgatcgt catctgccgg
     2101 ataaagccat tgacgtcatc gacgaagcgg gcgctcgcgc acgcctgatg ccggtaagca
     2161 aacgcaagaa aaccgttaat gtggcggata ttgagtccgt ggtggcccgt attgcacgca
     2221 ttccagagaa gagtgtttct cagagtgacc gcgataccct gaaaaacctc ggcgatcgcc
     2281 tgaaaatgct ggtcttcggt caggataaag ccattgaggc gctgactgaa gccattaaga
     2341 tggcgcgtgc aggtttaggt cacgaacata aaccggtcgg ttcgttcctg tttgccggcc
     2401 ctaccggggt cgggaaaaca gaggtgacgg tacagctttc gaaagctttg ggcattgagc
     2461 ttctgcgctt tgatatgtcc gagtatatgg aacgccatac cgtcagccgt ctgattggtg
     2521 cgcctccggg atacgttggt tttgatcagg gcggtttgct gactgatgcg gtcatcaagc
     2581 atccacatgc ggtgctgctg ctggacgaaa tcgagaaagc gcacccggac gtgttcaata
     2641 ttctgttgca ggtgatggac aacggtacgc tgaccgataa caacggacgc aaagcggact
     2701 tccgtaacgt ggtattggtg atgaccacta acgctggggt acgagaaact gagcgcaaat
     2761 cgattggtct tatccaccag gacaacagca ccgatgcgat ggaagagatc aagaagatct
     2821 ttacgccgga gtttcgtaac cgtctcgaca acattatctg gttcgatcat ctctccaccg
     2881 acgtgatcca tcaggtagtg gataaattca tcgtcgagtt gcaggttcag ttggatcaga
     2941 aaggtgtttc tctggaagtg agccaggaag cgcgtaactg gctggccgag aaaggttacg
     3001 accgggcaat gggcgcacgt ccgatggcgc gtgtcatcca ggacaacctg aaaaaaccgc
     3061 tcgccaacga actgttgttt ggttcgctgg tggacggcgg tcaggtgacg gttgcgctgg
     3121 ataaagagaa aaatgagctg acttatggat tccagagtgc acaaaagcac aaggcggaag
     3181 cagcgcatta atctgattgt caggtaggtt ggtgaagtcc gtaatctcga aagaggttac
     3241 ggactttttg tttgtaggct ggggcggtga aaaccctatt tttggaggtg aaggtaagtt
     3301 gttgataatt aatggtgctg gaaggtaaga ataaaaaagg gtggcagcag gaaaatgaga
     3361 tggttttgct ttattaataa cggactaaac gtgtagtatt tgagttcact gccgtacagg
     3421 cagataaaat gcgaaaaaaa actcgtactt tcgtacgagc ttttctttaa atatggcggt
     3481 gaggggggat tgactcgctt cgctcgccct gcgggcagcc cactcactgt gttcgtggtc
     3541 tgtccaactg gctgcgccag ttgtcgaacc ccggtcgggg cttctcatcc cccctgg
//
LOCUS       DAOTRK010000254         3570 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.257, whole genome shotgun sequence.
ACCESSION   DAOTRK010000254 DAOTRK010000000
VERSION     DAOTRK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.257"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1195)
                     /locus_tag="QIE41_004614"
     CDS             complement(<1..1195)
                     /locus_tag="QIE41_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDS3533389.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRHQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            1388..1891
                     /locus_tag="QIE41_004615"
     CDS             1388..1891
                     /locus_tag="QIE41_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS3533390.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1937..2353)
                     /gene="rraB"
                     /locus_tag="QIE41_004616"
     CDS             complement(1937..2353)
                     /gene="rraB"
                     /locus_tag="QIE41_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDS3533391.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            2515..3519
                     /gene="argF"
                     /locus_tag="QIE41_004617"
     CDS             2515..3519
                     /gene="argF"
                     /locus_tag="QIE41_004617"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDS3533392.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     IAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT         1081 a          822 c          759 g          908 t
ORIGIN      
        1 acagaaatgg taaagaaggc ataactcccc gtgaaatact ggctaagaag cctttatctg
       61 gttgttcttc tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt
      121 ttgcctgcca gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc
      181 cggatatcac caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc
      241 gaaaacgaat acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca
      301 ccgttaaata actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca
      361 ttttacgttg catttccata aattgctgta aattctcaat atcatcattt gcatacccag
      421 aactatcata ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag
      481 gtaataaagc taaaaatgcg agaatggcat attttataca atatgtagta tctaatttca
      541 ccttgaaacg atgtataccg aaactggtat tactccagag aaaactcatt accagactat
      601 ataaagtacc attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag
      661 atataataac actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag
      721 tccccatccc aatggccatt aaaatcggca agaaaaaggt cacccaccag aaccctttca
      781 tagagcaatt aaaactaaat cttacgccgt tgagactggt cattaaggcc tgatgacgca
      841 gtccttttgc tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa
      901 caagcggcat atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa
      961 gacaactaac aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt
     1021 ccatattagc ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta
     1081 gcgttataat cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac
     1141 catgaaaaac aaacgaatgg gacggaacat ccatttcatt aataacttga gccataaaaa
     1201 ttccctgaat taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc
     1261 cacgttacaa tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc
     1321 ttgcgcacgt aagaagagag tggtattaaa caaatgtata attactttat ttttattggg
     1381 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga
     1441 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
     1501 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
     1561 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
     1621 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
     1681 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
     1741 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
     1801 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
     1861 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctataggg
     1921 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
     1981 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
     2041 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
     2101 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
     2161 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
     2221 tttccagatc gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc
     2281 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
     2341 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     2401 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     2461 catccatata aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc
     2521 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga acttaacagc
     2581 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     2641 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     2701 ttcgaagttg ccgcatatga ccagggtgct cgcgttactt atctcggccc aagcggcagc
     2761 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     2821 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     2881 gtgccggtat ggaacggcct gaccaatgag tttcacccaa cgcagctgct ggcggatctc
     2941 ctcaccatgc aggagcattt gcccggcaaa gcgttcaacg aaatgacgct ggtctatgca
     3001 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     3061 gatttgcgtc tggtcgcgcc acaggcgtgc tggccagaag ctgcgctggt tacggaatgc
     3121 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatat agctaaagga
     3181 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa
     3241 aaatgggctg aacgcattgc attgctgcgt gattatcagg tgaacagcaa gatgatgcag
     3301 ttgactggta accctgaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     3361 acgacgcttg gcaagaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     3421 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     3481 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     3541 cgggaaatac aaaaaatatc ccggcatcat
//
LOCUS       DAOTRK010000255         3556 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.258, whole genome shotgun sequence.
ACCESSION   DAOTRK010000255 DAOTRK010000000
VERSION     DAOTRK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.258"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            70..834
                     /gene="ycaL"
                     /locus_tag="QIE41_004618"
     CDS             70..834
                     /gene="ycaL"
                     /locus_tag="QIE41_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDS3533393.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGAASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFELLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            1007..1690
                     /gene="cmk"
                     /locus_tag="QIE41_004619"
     CDS             1007..1690
                     /gene="cmk"
                     /locus_tag="QIE41_004619"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDS3533394.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1801..3474
                     /gene="rpsA"
                     /locus_tag="QIE41_004620"
     CDS             1801..3474
                     /gene="rpsA"
                     /locus_tag="QIE41_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDS3533395.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
BASE COUNT          927 a          839 c          952 g          838 t
ORIGIN      
        1 tagcgtcgtt tgtcaatgta agttattgat acataatatt tatatatgat taatcaacgg
       61 atgattcaca tgaagaatac taaattactg ctggcgattg cggcctctgc agcattactg
      121 acagggtgtc aaaataccca cggtattgat accaatatgg ctatcagctc aggtttaaat
      181 gcctataaag cagcaacgtt aagcgatgct gatgcaaaag cgattgccaa tcagggctgt
      241 gccgaaatgg acagcggcaa tcaagtcgca agtaaatcca gcaagtacgg taaacgtctg
      301 gcaaaaatcg ccaaagcatt gggtaacaat attaatggca cgccggtcaa ctataaggtt
      361 tatatgacca gcgacgtcaa cgcatgggcg atggcgaacg gctgtgttcg tgtctacagt
      421 ggcctgatgg acatgatgaa cgacaacgaa attgaaggcg ttctgggtca tgaactgggc
      481 cacgtcgcgt tgggtcactc gctggctgaa atgaaagctt cttatgcgat cgttgccgca
      541 cgtgatgcga tttccgctac cagcggtgcg gcttcccagc tttctcgctc acaattaggt
      601 gatatcgcag aaggcgcgat caatgctaaa tactcccgcg ataaagagtc cgaagcagat
      661 gatttctcct ttgaactgtt gaagaaacgt ggcatcagca cccaggggct ggttggcagc
      721 tttgaaaaat tggccagcct ggatggcggt cgtacccagt ccatgtttga ctctcaccca
      781 ccatcaacag agcgtgcgca acacatccgt gatcgtatcg cctctggtaa gtaaatcatt
      841 gtcatctttc gggctggtct tctgccagcc cgctataatt gcgcaataaa tccccatctg
      901 aatacagaca aaactggttt ttgcacacaa cgttaacgat ttgtggcgtc ggcgcgtata
      961 atgcgcgcgg ttatgttaac ggtacgcctg ttttaaggag ataaagatga cggcaattgc
     1021 cccggttatt accattgatg gcccaagcgg tgcagggaaa ggcaccttgt gtaaggctat
     1081 ggcggaagcg ttgcaatggc atctgctgga ctcgggtgca atttatcgcg tactggcact
     1141 ggcggcatta catcaccatg ttgatgttgc atcggaagat gcgctcgtgc cgctggcatc
     1201 ccatctggat gtacgttttg tgtcgaccaa tggcaatctg gaagtgatcc tcgaagggga
     1261 agatgtcagc ggcgaaattc gtactcagga agtggcgaat gctgcttcac aagtcgcggc
     1321 attcccacgc gttcgtgaag cattattgcg tcgccaacgc gcgtttcgcg aattaccagg
     1381 cttgattgcc gatggccgcg atatgggaac ggtggtattc cctgatgcgc cagtgaaaat
     1441 tttccttgac gcctcctcgg aagaacgtgc gcatcgccgc atgctacagt tgcaggagaa
     1501 gggctttagt gttaactttg agcgcctttt ggccgagatc aaagaacgcg acgaccgcga
     1561 tcgtaaccga gcggtagcgc cactggttcc ggcagccgat gctttagtgt tggattccac
     1621 caccttaagc attgagcaag tgattgaaaa agcgctacaa tacgcgcgcc agaaattggc
     1681 tctcgcataa gcgaccgaat ttgcagtacc cccgttgcaa tggaatgaca gcgggtatgt
     1741 taaacaaccc catccggcat ggagccaggt ggacgttaaa tataaacctg aagattaaac
     1801 atgactgaat cttttgctca actctttgaa gagtccttaa aagaaatcga aacccgcccg
     1861 ggttctatcg ttcgtggcgt tgttgttgct atcgacaaag acgtagtact ggttgacgct
     1921 ggtctgaaat ctgagtccgc catcccggct gagcagttca aaaacgccca gggcgagctg
     1981 gaaatccagg taggtgacga agttgacgtt gctctggacg cagtagaaga cggcttcggt
     2041 gaaactctgc tgtcccgtga gaaagctaaa cgtcacgaag cctggatcac gctggaaaaa
     2101 gcttacgaag atgctgaaac tgttaccggt gttatcaacg gcaaagttaa gggcggcttc
     2161 actgttgagc tgaacggtat tcgtgcgttc ctgccaggtt ctctggtaga cgttcgtccg
     2221 gtgcgtgaca ctctgcacct ggaaggcaaa gagcttgaat tcaaagtaat caagctggat
     2281 cagaagcgca acaacgttgt tgtttctcgt cgtgccgtta tcgaatccga aaacagcgca
     2341 gagcgcgatc agctgctgga aaacctgcag gaaggcatgg aagttaaagg tatcgttaag
     2401 aacctcactg actacggtgc attcgttgat ctgggcggcg ttgacggcct gctgcacatc
     2461 actgatatgg cctggaaacg cgttaagcat ccgagcgaaa tcgtcaacgt gggcgacgaa
     2521 atcactgtta aagtgctgaa gttcgaccgc gaacgtaccc gtgtatccct gggcctgaaa
     2581 cagctgggcg aagatccgtg ggtagctatc gctaaacgtt atccggaagg taccaaactg
     2641 actggtcgcg tgaccaacct gaccgactac ggctgcttcg ttgaaatcga agaaggcgtt
     2701 gaaggcctgg tacacgtttc cgaaatggat tggaccaaca aaaacatcca cccgtccaaa
     2761 gttgttaacg ttggcgatgt agtggaagtt atggttctgg atatcgacga agaacgtcgt
     2821 cgtatctccc tgggtctgaa acagtgcaaa gctaacccgt ggcagcagtt cgcggaaacc
     2881 cacaacaagg gtgaccgtgt tgaaggtaaa atcaagtcta tcactgactt cggtatcttc
     2941 atcggcctgg acggcggcat cgacggcctg gttcacctgt ctgacatctc ctggaacgtt
     3001 gcaggcgaag aagcagttcg tgaatacaaa aaaggcgacg aaatcgctgc agttgttctg
     3061 caggttgacg cagaacgtga acgtatctcc ctgggcgtta aacagctcgc agaagatccg
     3121 ttcaacaact gggttgctct gaacaagaaa ggcgctatcg taaccggtaa agtaactgca
     3181 gttgacgcta aaggcgcaac cgtagaactg gctgacggcg ttgaaggtta cctgcgtgct
     3241 tctgaagcat cccgtgaccg cgttgaagac gctaccctgg ttctgagcgt tggcgacgaa
     3301 gttgaagcta aattcaccgg cgttgatcgt aaaaaccgcg caatcagcct gtctgttcgt
     3361 gcgaaagacg aagctgacga gaaagatgca atcgcaactg ttaacaaaca ggaagatgca
     3421 aacttctcca acaacgcaat ggctgaagct ttcaaagcag ctaaaggcga gtgattctct
     3481 gactcttcgg gatttttatt ccgaagtttg ttgagtttac ttgacagatt gcaggtttcg
     3541 tcctgtaatc aagcac
//
LOCUS       DAOTRK010000256         3548 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.259, whole genome shotgun sequence.
ACCESSION   DAOTRK010000256 DAOTRK010000000
VERSION     DAOTRK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.259"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            410..1978
                     /gene="cydA"
                     /locus_tag="QIE41_004621"
     CDS             410..1978
                     /gene="cydA"
                     /locus_tag="QIE41_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDS3533396.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1994..3133
                     /gene="cydB"
                     /locus_tag="QIE41_004622"
     CDS             1994..3133
                     /gene="cydB"
                     /locus_tag="QIE41_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDS3533397.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3148..3261
                     /gene="cydX"
                     /locus_tag="QIE41_004623"
     CDS             3148..3261
                     /gene="cydX"
                     /locus_tag="QIE41_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDS3533398.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3261..>3548
                     /gene="ybgE"
                     /locus_tag="QIE41_004624"
     CDS             3261..>3548
                     /gene="ybgE"
                     /locus_tag="QIE41_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDS3533399.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFF"
BASE COUNT          739 a          847 c          945 g         1017 t
ORIGIN      
        1 taacaataaa attaccaatg atctatggtt tatacataat taacatattt ggttgatttt
       61 gatataaatc aatttttaat tcttgaaaaa aatctgcaaa acacgataaa ttgtcgtaga
      121 tcaaattggt gagatcgtga caatttcgct ataaattgat cactgtcgaa aaatgcaaat
      181 ttgctcaata aaaacctgtt tattgtaagg attttgcggc gtaatataat tacgggatca
      241 atttggttta ttcattaaca tatttgtaac ttttttatca ttgtcttttc ctcttttgat
      301 gaaatggcaa cgatagaggc gagcaaataa ttttgtattg gggcatgcgt gtgacccttt
      361 ctaacggggt tcactctcgg agtcttcatg cgatgagcaa ggagtcatga tgttagatat
      421 agtcgaactg tcgcgcttac agtttgcctt gaccgcgatg taccacttcc tttttgtgcc
      481 actgacgctc ggtatggcgt tcctgctggc cattatggaa acggtctacg tcctttccgg
      541 caaacagatt tataaagata tgaccaagtt ctggggcaag ttgtttggta tcaacttcgc
      601 tctgggtgtg gctaccggtc tgactatgga gttccagttc gggactaact ggtcttacta
      661 ttcccactat gtaggggata tcttcggtgc gccgctggca atcgaaggtc tgatggcctt
      721 cttcctcgaa tccacctttg taggtctgtt cttcttcggt tgggatcgtc tgggtaaagt
      781 tcagcatatg tgtgtcacct ggctggtggc gctcggttca aacctgtccg cactgtggat
      841 tctggttgcg aacggctgga tgcaaaaccc aatcgcgtcc gatttcaact ttgaaactat
      901 gcgtatggag atggtgagct tctccgagct ggtgcttaac ccggttgctc aggtgaaatt
      961 cgttcacact gtagcgtctg gttatgtgac tggcgcgatg ttcatcctcg gtatcagcgc
     1021 atggtatatg ctgaaaggtc gtgacttcgc cttcgctaaa cgctcctttg ctatcgctgc
     1081 cagcttcggt atggctgccg ttctgtctgt tattgttctg ggtgatgaat ctggttacga
     1141 aatgggcgac gtgcagaaaa ccaaactggc tgctattgaa gccgagtggg aaacgcaacc
     1201 tgcgcctgct gcctttactc tgttcggcat tcctgatcag gaagaggaga cgaacaaatt
     1261 tgcgatccag atcccttacg cgctgggtat cattgcaacg cgttccgtgg atactccagt
     1321 tatcggcctg aaagaactga tggtgcagca tgaagaacgc attcgtaacg ggatgaaggc
     1381 gtactctctg ctcgagcaac tgcgttctgg ttctaccgac caggcggttc gtgaccagtt
     1441 caatagcatg aagaaagacc tcggttacgg tctgctgctg aaacgctata cgccaaacgt
     1501 ggctgatgcg actgaagcgc agattcaaca ggcaaccaaa gactccattc cgcgtgtagc
     1561 gccgctgtac ttcgcgttcc gtatcatggt ggcgtgtggc ttcctgctgc tggcaatcat
     1621 cgcgctctct ttctggagtg tcatccgcaa ccgcattggc gagaaaaaat ggctgctgcg
     1681 cgccgcgctg tacggtattc cgctgccgtg gattgctgta gaagcgggct ggtttgtggc
     1741 tgaatatggc cgccaaccgt gggctatcgg tgaagtgctg ccgacagctg tggcgaactc
     1801 gtcactgacc gcaggcgatc tcatcttctc aatggtgctg atttgcggcc tgtataccct
     1861 gttcctggtg gcagaattgt tcttaatgtt caagtttgca cgcctcggcc caagcagcct
     1921 gaaaaccggt cgctatcact ttgagcagtc ttccacgact actcagccgg cacgctaaga
     1981 caggagtcgt caaatgatcg attatgaagt attgcgtttt atctggtggc tgctggttgg
     2041 cgttctgctg attggttttg cagtcactga cggtttcgac atgggggtgg gcatgctcac
     2101 ccgtttcctc ggtcgtaacg acaccgagcg tcgaattatg attaactcca tcgcaccaca
     2161 ctgggacggt aaccaggttt ggctgatcac cgcaggcggc gcactctttg ctgcctggcc
     2221 gatggtctat gccgctgcgt tctccggctt ctatgtggcg atgatcctcg tgctggcgtc
     2281 tttgttcttc cgtccggtcg gttttgacta ccgctccaag attgaagaaa cccgctggcg
     2341 taacatgtgg gactggggca tcttcattgg tagcttcgtt ccgccgctgg tgattggtgt
     2401 agcattcggt aacctgttgc agggcgtacc gttcaacgtt gatgaatatc tgcgtctgta
     2461 ctacaccggt aacttcttcc agctgcttaa cccgttcggc ctgctggctg gcgtggtgag
     2521 cgtagggatg atcattactc agggcgcaac ctatctgcaa atgcgtaccg tgggcgaact
     2581 gcacctgcgt acccgtgcaa cggctcaggt ggcggcactg gtgacactgg tctgcttcgc
     2641 actggctggc gtatgggtga tgtacggtat cgatggttat gttgtgaaat caacaatgga
     2701 ccattacgca gcctctaacc cgttgaataa agaagtggtt cgtgaagctg gcgcatggct
     2761 ggttaacttc aacaacacgc caattctgtg ggctattccg gcactgggtg tagttctgcc
     2821 gctgctgacc atcctgactg ctcgtatgga taaagctgcg tgggcgtttg tgttctcctc
     2881 cctgacgctg gcctgcatca tcctgactgc cggtatcgca atgttcccgt ttgtgatgcc
     2941 gtccagcacc atgatgaacg caagtctgac aatgtgggat gcaacttcca gccagctgac
     3001 gcttaacgtc atgacctggg ttgcggtggt tctggtaccg atcattctgc tctacaccgc
     3061 ctggtgttac tggaaaatgt tcggccgtat caccaaagaa gatattgaac gtaacaccca
     3121 ctctctgtac taagtaagga gctaaaaatg tggtatttcg catggattct gggaacgctt
     3181 cttgcctgtt cgtttggggt aatcaccgcg ctggcgcttg agcacgtcga atcaggcaaa
     3241 gccggtcaag aagacatctg atgagtaaga ttatcgcaac cttgtatgcg gtaatggaca
     3301 agcgccccct gcgggcgctt tccttcgtga tggcgcttct gttagcagga tgtatgtttt
     3361 gggacccatc acgtttcgcc gcgaagacca gtgaactgga aatctggcat ggtttattgc
     3421 tgatgtgggc cgtctgtgct ggtgttattc acggcgtggg ctttcgtccg cagaaggttc
     3481 tctggcaagg gattttttgc cctttgcttg ccgatattgt tctcattgtc gggctgattt
     3541 tcttcttc
//
LOCUS       DAOTRK010000257         3494 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.260, whole genome shotgun sequence.
ACCESSION   DAOTRK010000257 DAOTRK010000000
VERSION     DAOTRK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.260"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..2985
                     /locus_tag="QIE41_004625"
     CDS             <1..2985
                     /locus_tag="QIE41_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDS3533400.1"
                     /translation="KPVQNCTIVLKARRTSSTVVVNTVASENPDEAGRYSMDVEHGQY
                     SVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEALRRFEQMVEEVSRN
                     ASAVAQNTAAAKKSASDASASASEAATHATDAAASARAANTSAGQAASSAQSASSSAG
                     TASTKAREAAKSAAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTATTKASEA
                     ATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSSETAAGQ
                     SASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAGKATEQA
                     TAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEATRQASA
                     AKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVALEDASTT
                     KKGIVQLSSATNSTSEKLAATPKAVKTVKDSSVQKTGDTMGGQLKISTINALRIFNQA
                     FGLIFRRSEDHLHLIPTNEGEGENGDIGSLRPFSINLRSGLVSIGNGLKVGGSVTGNL
                     TGNADTATKIKTARKIGGVAFDGSADINLPGVNATGNQNTTGNAATATKLQAARTING
                     VSFDGSANITLTPSNIGALALTGGTLSGGLTAAGEVISRSANGLRIAYGNYGFFIRND
                     GSNTYFMLTDSGNSLGTHNSLRPFIISNHTGNVTIATKLNASGGITGSLSGNASTATK
                     LQTARTINGVKFDGSANIEAFPPGVPLPWPSDTPPAGYAIMQGQTFDKAAYPKLAIAY
                     PSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTK
                     STNNTGAHTHSLSGSTGSAGVHTHGNGIRWPGGGGSALAFYDGGGFTYVQNSQYQVSP
                     ETSSYRSYYQRIQTQSAGAHTHSLSGTAASSGAHAHTVGIGAHTHSVAIGSHGHTITV
                     NAAGNAENTVKNIAFNYIVRLA"
     gene            2985..>3494
                     /locus_tag="QIE41_004626"
     CDS             2985..>3494
                     /locus_tag="QIE41_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDS3533401.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDELFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKY"
BASE COUNT          976 a          858 c          938 g          722 t
ORIGIN      
        1 aaaccagtac agaactgcac cattgtgctg aaggccagac gaaccagcag cacggtggtg
       61 gtgaacacgg tggcctctga aaatccggat gaagccggac gttacagcat ggatgttgag
      121 catggtcagt acagcgtcac cctgctggtt gaaggttttc cgccttcaca tgccgggacc
      181 attaccgtct atgaaggttc cagaccaggt acgctgaatg attttctcgg tgccatgacg
      241 gaggatgatg tccgaccgga ggcactgcgc cgctttgagc agatggtgga agaggtgtca
      301 cgtaacgcct ccgcggttgc acagaatacg gcagccgcga aaaaatcagc cagcgatgcc
      361 agtgcatcag ccagcgaggc ggcaactcat gcaaccgatg ctgcagcctc agcacgtgcc
      421 gccaacacgt cagccggaca ggccgcgtcg tcggctcagt cagcgtcttc cagcgcagga
      481 acggcatcga caaaggcccg tgaagcagca aaaagtgctg ctgctgcaga gtcatcaaaa
      541 agcgcggcag ctaccagcgc cagtgccgcg aaaacgtcag aaacgaatgc cgcagcatca
      601 caaaaatcgg cagccacttc tgcatccaca gcgaccacga aggcgtcaga agctgccacc
      661 tcggcacggg gtgcggcggc ctcaaaagag gcagcgaaat cttcagaaac gaatgcatca
      721 tcaagtgcca gtagtgcagc ttcctcggca acggcggcag gaaattccgc gaaggcggca
      781 aaaacgtccg agacgaacgc taagtcttct gaaacagcag cgggacagag cgcctcagct
      841 gcggcaggtt caaaaacagc ggctgcatta tctgccagtg ccgcgtcaac aagtgccggg
      901 caggcctcag ccagtgccac cgccgccgga aaatcggcag aaagcgccgc atcatccgct
      961 tcaacagcca caacgaaggc tggcaaagcc actgagcaag ccactgcagc agcgaggtct
     1021 gcttctgcag caaaaacctc tgaaacaaat gcaaagactt cagcagacaa tgctgcttcc
     1081 tctaaggcgg cagccgcatc gtcagccagt tcagcggcgt catcggcatc atctgcgtct
     1141 gcttcaaaag atgaggcgac cagacaagcg tcagcagcga aaggtagtgc cacgacagca
     1201 acaacgaaag catcagaggc ggcaggcagt gcgacggctg catctcagag caaagttgct
     1261 gctgaatccg cggcaacgcg cgccgagaca gcagcaaaac gggcagagga tattgcatcc
     1321 gccgtagcgc tggaggatgc gagcacgacg aaaaagggga tagtacagct cagcagtgca
     1381 acaaacagca ccagtgaaaa gctggcggca acgccaaagg cagttaaaac tgttaaagat
     1441 agttcagttc aaaaaactgg cgacacaatg ggagggcagt taaaaatcag cacgataaat
     1501 gctcttcgaa tattcaacca agcctttggc cttattttta ggcgttccga agatcatctt
     1561 catcttattc cgactaatga aggggagggg gaaaatggag acatcggttc attaagacca
     1621 ttctctataa acttaagatc agggttggtg tccatcggta atggactaaa agttggtggt
     1681 agtgttactg gtaatttgac cggaaacgca gatactgcga ccaagatcaa gacagcacgt
     1741 aagattgggg gcgtggcatt tgatggatcg gcagatatca acttgcctgg agtcaacgct
     1801 accggtaatc aaaacactac aggtaatgct gcgaccgcca cgaaacttca ggcagccaga
     1861 acgattaacg gtgtgtcatt tgatggtagt gcaaatatca cattgacccc ttcaaatatt
     1921 ggggcattgg cattaactgg agggactctt tcaggtggtt taactgctgc tggtgaggtt
     1981 atttcaaggt cagcaaatgg tctgcgtatt gcctatggca actatggatt ctttatccga
     2041 aatgatggat caaacacata ttttatgttg acagattcgg gtaacagtct tggtacgcac
     2101 aatagcttaa ggccgtttat aattagtaac catactggca atgttacaat tgcaactaaa
     2161 ttaaacgcga gtggtggtat cactggatct ttatcgggta atgcaagcac agcaaccaaa
     2221 ttgcaaactg caaggacaat taacggcgta aaatttgacg gctcggcaaa tattgaagcg
     2281 tttccgccag gtgttccgct gccgtggcca tcagatacac cacctgcagg ttatgcaatc
     2341 atgcaggggc agacgtttga taaggcagca tatccgaaac tggctattgc ctatccttca
     2401 ggtgttattc cagatatgcg cggctggaca atcaagggca aacccgccag tggccgggcc
     2461 gtattgtctc aggaacagga cggcattaaa tcgcacaccc acagcgccag cgcatccagt
     2521 acggatttag ggacgaaaac cacatcgtcg tttgattacg gcactaaatc cacgaataac
     2581 accggggcgc atacgcacag tctgagtggc tctacggggt ctgccggtgt tcatactcat
     2641 ggtaatggta ttcgttggcc aggaggcggc ggttctgcgt tagcatttta tgatggcggt
     2701 gggttcactt atgtccagaa ttcacagtat caagtaagcc cggagacttc ttcctataga
     2761 tcgtattatc aacgtattca gacacagtca gcaggtgctc atacccactc gctgtctggt
     2821 actgcagcaa gttctggcgc acatgcacat actgtaggta ttggtgcgca tacgcactcc
     2881 gttgcgattg gttcacatgg acacaccatc accgttaacg ctgcgggtaa cgcggaaaac
     2941 accgtcaaaa acatcgcatt taactatatt gtgaggcttg cataatggca ttcagaatga
     3001 gtgaacaagc acggaccata aaaatttata atctgctggc cggaactaat gaatttattg
     3061 gtgaaggtga cgcatatatt ccgcctcata caggtctgcc agcaaacagt accgatattg
     3121 caccaccaga tattcctgct ggctttgtgg ctgttttcaa cagtgatgag gcatcgtggc
     3181 atctcgttga agaccatcgg ggtaaaacgg tttatgacgt agcgtcaggg gacgagttat
     3241 ttatttctga actcggtccg ttaccggaaa atgttacctg gttatcgccg gaaggggagt
     3301 ttcagaagtg gaacggcaca gcctgggtga aggatacgga agcagaaaaa ctgttccgga
     3361 tccgggaggc ggaagaaaca aaaaacaacc tgatgcaggt agccagtgag catattgcgc
     3421 cgcttcagga tgctgcagat ctggaaattg caacggagga agaaacctca ttgctggaag
     3481 cctggaaaaa gtat
//
LOCUS       DAOTRK010000258         3462 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.261, whole genome shotgun sequence.
ACCESSION   DAOTRK010000258 DAOTRK010000000
VERSION     DAOTRK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.261"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..2355
                     /locus_tag="QIE41_004627"
     CDS             <1..2355
                     /locus_tag="QIE41_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDS3533402.1"
                     /translation="KPIQNCTIQLKAKRNSTTVLVNTVASENPDEAGRYSMDVEYGQY
                     SVILLVEGFPPSHAGIITVYEGSRPGTLNDFLGAMTEDDVMPEALRRFEAMVEEAARN
                     AEAASQSAAVAKKSETAAASSKNAAKTSETNAANSAQAAASSQTASANSATAAKKSET
                     NAKNSETAAKTSETNAKSSQTAAKTSETNAKASETAAKNSQAAAAESESAAAGSATSA
                     AGSATAAANSQKAAKTSETNAKSSQTAAKTSETNAKASETAAKSSQDAAAQSESAAAS
                     SASAAAASATASANSQKAAKTSETNAKVSETAAANSAKASAASQTAAKASEDAAREYA
                     SQAAEPYKYVLQPLPDVWIPFNDSLDMITGFSPSYKKIVIGDDEITMPGDKIVKFKRA
                     SKATYINKSGVLTEAAIDEPRFERDGLLIEGQRTNYMLNSESPASWGRSSNMDVPETG
                     TDNFGFTYGKFVCNDSLIGQTSAINMASIAATKSVDVSGDNKHVTTSCRFKTELQVRL
                     RIRFDKYDGSATTFLGDAYIDTQTLEINMTGGAASRITARVRKDEATGWIFAEATIQA
                     IDGELKIGSQIQYSPKQSGATVSGDYIYLATPQVEDGPCVSSFIISGATAATRASDMV
                     TIPTENNIYNRPLTCLVEVNRNWGDIPPNVAPRIFDFSGVPPIESITYAFNTTERYYG
                     QLYMQTYKASTSSYVSSLFTGRTDVRKFIGGFNIYSDGTKRVVSNGEATKTIETEWTG
                     VKTRTFIRIGGQATSGTRHLFGHLRNLRLWHKELTDAQMGESIK"
     gene            2352..2633
                     /locus_tag="QIE41_004628"
     CDS             2352..2633
                     /locus_tag="QIE41_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533403.1"
                     /translation="MKDLTLKFADRADFSAFMDSTGYYDDESMQDDILIDVIGNVYKE
                     TGELNEDGEPVCVKEDGYFVNVRIINDVKTPSIFDEYVVAVEHQLRGWM"
     gene            2643..>3462
                     /locus_tag="QIE41_004629"
     CDS             2643..>3462
                     /locus_tag="QIE41_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDS3533404.1"
                     /translation="MATSTVIPDDIKTLKSDVSKLKNDQGSYATKSYVDSKDETVGDW
                     SASWYQQVLPTSGAIFGRKLRSTHRTAGVEDAYCELYLKKWIDSPGNAMARLNLNDNG
                     TNICWDFTNLYGGTMIFPGDSGYLKMGNCLMSYSKRGSNALIKFDYTDTLQIKYANHG
                     STMTLNTQGTAYAGVTAQLWGNSSRPVVYEVGVDGGAYMFYAQKNTDNTYMLSVNGAC
                     HATAFNQHSDRDLKDNIQVIDNATDRIRKMNGYTYTLKENGMPYAGVIAQEALEA"
BASE COUNT         1062 a          753 c          871 g          776 t
ORIGIN      
        1 aaaccaatac agaactgcac tattcaactg aaggcaaagc gtaacagcac cactgtactg
       61 gtgaacacgg tggcctctga aaatccggat gaagccgggc gttacagcat ggacgttgag
      121 tatggccagt atagcgttat cctgctggtt gaaggttttc cgccttcaca tgccgggatc
      181 attaccgtct atgaaggttc cagaccaggt acgctgaatg attttctcgg tgccatgacg
      241 gaagatgatg tcatgccgga ggcattgcgt cgttttgagg caatggtgga agaagcggca
      301 cgcaacgccg aagccgcctc tcagagcgca gcggtggcaa agaaatccga aactgcagcg
      361 gcatcatcga agaacgcggc gaaaacctca gaaacgaatg cagctaacag cgcacaggcg
      421 gcagcgtcct cgcagactgc atcggcaaac tccgcgacag cagccaaaaa atcagaaacc
      481 aacgcgaaaa acagcgagac agccgcaaag acgagcgaaa ccaacgcaaa gtccagccag
      541 acggcagcga aaaccagcga aacgaatgcc aaagccagtg aaactgcggc gaaaaatagc
      601 caggctgcag cagctgaaag cgagagcgca gcagccggtt ctgcgacttc agcagctgga
      661 tcagcaactg ctgcggctaa cagccagaaa gctgcgaaga cgagtgaaac taacgcaaag
      721 tccagccaga cggcagcgaa gaccagcgaa acgaatgcca aagccagtga aactgcggcg
      781 aaaagcagtc aggatgcagc ggcccaaagc gagagtgccg cagccagttc tgcaagcgcg
      841 gcggctgctt ctgccactgc atcagctaac agtcaaaaag cagcaaaaac cagtgaaacc
      901 aacgcaaagg tgagcgaaac agcggctgcg aactcagcga aagcatcggc agcaagccag
      961 acggcagcta aagcaagcga agatgcagcc agagagtacg caagccaggc tgcggagccg
     1021 tataaatatg tcttacagcc gctgcctgat gtgtggatac catttaacga ttcgctggat
     1081 atgattacgg gcttttcgcc atcatataaa aaaattgtta ttggtgacga tgaaataacg
     1141 atgcctggcg acaagattgt taagtttaaa cgtgcatcga aagcaaccta tattaacaaa
     1201 tctggtgtgc tgacagaggc tgccattgac gagccacgat ttgaacgtga tggcctgctt
     1261 attgaggggc aaagaacaaa ctacatgctc aattcggaaa gccctgccag ttgggggcga
     1321 tcgtcaaata tggatgtgcc cgaaacaggg acggataatt ttggttttac ctatggaaag
     1381 tttgtctgca acgattctct gattgggcaa acctcagcca ttaatatggc atcaattgct
     1441 gcaacaaagt cagttgatgt ctcaggcgat aataaacacg tgacaacctc atgtcgtttt
     1501 aaaacagaac tgcaggtaag gttgcgtatc cggtttgata aatatgacgg tagcgcaaca
     1561 acttttcttg gtgatgcgta tattgataca caaacgcttg aaattaatat gacaggcggt
     1621 gctgcctcaa ggatcacagc gagagtcaga aaggacgaag ctaccggatg gatttttgca
     1681 gaggcaacaa ttcaggcaat tgatggggag ttaaaaatag gctctcagat acagtattct
     1741 cctaagcaga gcggggcaac cgtatctggt gactatattt atctggccac cccacaagta
     1801 gaagatgggc cttgtgtatc atcttttatt atatcaggag cgacggcggc gactcgtgcg
     1861 agtgatatgg ttacgatccc gaccgaaaac aacatttata acagaccgct tacttgtttg
     1921 gtcgaggtta acaggaattg gggcgatatt ccccctaatg tagcaccgcg tatttttgat
     1981 ttttctggtg tgccgcctat tgagtcaatc acatacgctt ttaacacaac cgagagatat
     2041 tacggtcagc tttatatgca aacttataaa gcgtcgacaa gtagttacgt ttctagtttg
     2101 tttactggtc gaacggatgt tcgaaaattc attggtggtt ttaatattta ttctgacggt
     2161 actaaacgag tagtttctaa cggtgaggct actaaaacca ttgaaacgga atggacgggc
     2221 gtaaaaacgc ggacctttat tagaatagga ggtcaagcca catcagggac acgtcatcta
     2281 ttcggccatt tgagaaatct tcgtctctgg cataaagaat taactgatgc gcaaatgggg
     2341 gagagtatta aatgaaagat ttaacactta aatttgccga cagggccgat ttttcggcct
     2401 ttatggatag cactggctat tatgatgatg agtcgatgca ggatgatatt cttattgacg
     2461 tgataggtaa cgtgtacaaa gaaaccggag aactgaatga agatggcgaa ccggtatgtg
     2521 ttaaggaaga cggatatttt gtaaacgtgc gcatcattaa tgatgtgaaa acaccgtcaa
     2581 tattcgatga atacgtggtt gctgttgagc atcaacttcg tggctggatg tgaggaagaa
     2641 aaatggctac atcgacagta attcctgatg acatcaaaac gctaaaatcc gacgttagca
     2701 aattaaaaaa cgatcaagga agctacgcaa caaaatcata tgtagacagc aaagatgaaa
     2761 ccgttggtga ctggtctgct tcatggtatc agcaggtatt gccaactagc ggagctatat
     2821 ttgggagaaa actccgctca actcacagga cggcaggtgt tgaggatgca tattgcgaac
     2881 tatacctcaa aaaatggata gacagtccag gtaacgcaat ggcgcgcctt aacctgaacg
     2941 ataacgggac aaacatttgc tgggacttta ccaaccttta tggcggtacg atgatttttc
     3001 ccggtgacag cggatacctc aaaatgggta actgccttat gtcatacagc aagcgtggaa
     3061 gtaacgcgct tattaaattt gattacaccg acacattaca gatcaaatat gccaatcatg
     3121 ggtcaaccat gacattaaac acacagggaa ccgcttatgc tggtgttact gctcaattgt
     3181 ggggcaactc cagccgtcct gttgtttatg aagtcggtgt tgatggtggc gcttatatgt
     3241 tctatgcgca gaaaaatacc gataacacct atatgttaag cgttaatggt gcatgtcatg
     3301 ccaccgcatt taaccagcat tccgaccggg atctgaaaga caacattcag gtgatcgata
     3361 atgcaaccga ccgcatccgt aaaatgaacg gctatacata cacgcttaaa gaaaacggta
     3421 tgccctatgc tggtgtcatt gcacaggaag ctctggaagc aa
//
LOCUS       DAOTRK010000259         3426 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.262, whole genome shotgun sequence.
ACCESSION   DAOTRK010000259 DAOTRK010000000
VERSION     DAOTRK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.262"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            339..1823
                     /locus_tag="QIE41_004630"
     CDS             339..1823
                     /locus_tag="QIE41_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDS3533405.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2145..2747)
                     /locus_tag="QIE41_004631"
     CDS             complement(2145..2747)
                     /locus_tag="QIE41_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533406.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(2834..3040)
                     /locus_tag="QIE41_004632"
     CDS             complement(2834..3040)
                     /locus_tag="QIE41_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDS3533407.1"
                     /translation="MLTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          889 a          746 c          890 g          901 t
ORIGIN      
        1 tttattttat tcttcttcag gagggggggg attcctgaag gcgttattcc cggtgttctg
       61 tttatgattc atcccggcag gaaaccggta tctgcaatac ccgggatgat gctttttgtt
      121 cgtacgtggt tgtgtaaaat ttgaatagtg gatgtgctct gaagggaagg aggggacaca
      181 cagattcatt ttgtctttct tcagcagcaa aacacactgt ggttattatt taaccattaa
      241 atcaacaaaa atttttacct catattgatt tgactgctat ttttgctatt aatagccaga
      301 attgcagtgc ttttatatct gtgtatagtg aggttactat gtcattcggt cagccttgtg
      361 atgagtttcc cctgagttcg ctgcctccac tcattcgtga tgccgttatt gaagctcagc
      421 aaattacgca ggctcccctt gggcttgtgg cagcgtctgc cctgggcgct gtttcacttg
      481 tctgccagaa tctgattgat gtctgcagac tgaatacact gcgggggccg gtctctcttt
      541 ttttcctgac gcttgccgag tccggagaac gaaaaaccgc agtggacaaa ttattaatga
      601 agcccctgta ccagcaggag atgcagttat attccagata caaaagtgaa ctggctgtct
      661 ggaaaaacaa ggaagagctt ttaaaggctc agaaaaaagc attgttgtca aaactgaata
      721 aagaactccg caaaggcgcg gatgagtcag aaaccctcag acaactggaa gtacttcaga
      781 aaaacagtgc ggaagaacct gtgaggtata aattcatttt taatgatgcc acgactgcgg
      841 caattaagaa tcagctttgt ggtaaatggc gttcagtggg tattatgtca gatgaagccg
      901 gtattatatt tgatggatat accctgtctg agttgccttt tattaacaaa atgtgggatg
      961 gctctgtatt gtctgtggac agaaagaatg aacccgaaca gatgattgaa aatgccagaa
     1021 tgacgttatc cctcatggtg caacccggtc tttttgaccg atatatggag cgaaaaggtt
     1081 ctgttgccag ggacagtggt tttcttgccc gttgtcttat cagtaaacct gcaaccacgc
     1141 aggggaaacg ctttattaac ggagctgtga ttccgggagg atcattaacg gcatttcatg
     1201 aacgattaat ggaactggcc agaggcagta ttgaaaaatc cagtgaggat gaacggtatt
     1261 gtttgcattt ttcgcctgaa gcgcaaaaaa tatttattga gcactataat gttctggagc
     1321 aggatttatc cccttccgga cccttaagcc cgtttcgggg acatgtttca aaaaaaacag
     1381 aaaacattgc aagaattgca gcgttatttc agtatttcag ctatggtgaa gggaaaatct
     1441 ctgccgatat catgacgtct gctgtcgtaa taagttcgtg gtacacagat gaatataaaa
     1501 aattatttgc gttacctgat gaatctgaac tgcagcagaa ggacgccgag gagctgttcg
     1561 actggctcat tgaggagtgc cgcggagaat gcccaccccg tgtcaggaaa aattatattc
     1621 tccagtgtgg cccgggtcgg ttcagaaaca ggaaaaaact gaacgcattg ctgaacattc
     1681 ttgaatctca gttcaggctt tctgtcgtgc cggaaggaaa aaccatgtat gttctgcttc
     1741 cccagatagc cagtttaaag ttgtctgatg tctctggtat ttttacctcg ggttatcact
     1801 acaataagct gagagcgaag taaccggtga gcagtttttc tccattatgc ttcagactta
     1861 gatgattgat tattatgggg atttcatcac cgatattcag atgtatgaat atcggtgatg
     1921 aactaaagct caggagctgc gtggtggtga tgaatcaaca ggcatataac tgatacccgc
     1981 cggtcagaag ttccagcctg cgactttcct gtctgcgtgc ccgcagtccg gcgttcaggt
     2041 gcagcgtgtg acagaacgca cggagagcga aacggcaggc agacaggccg ttcagtcagc
     2101 cctggcaccc tgcccggata ccagcttccc tgaaacaact ggcatcaggt gcgcttatcg
     2161 tcaaccgttg tgcggtatgc caggagcacg tactgatcca tcagatccgt cagtgctgcg
     2221 ctctgcttct gacggttgat gatccattcc tccagctctt tttctgattt ccgtttcaga
     2281 ttctccgtcc agtcgtcaag gcagtttccg gtcattaaat caagccagca aagccagacc
     2341 agtttgaggc gcaactccgt gccaccaaac tgacggaaga ggctgatcag tttatttgcc
     2401 agcgaacgct gcctcagacg ttcacattca gacaggagaa tgccttccag catcggatga
     2461 tgaaccagca gatgttcact ggtgcataaa ttcactatca gatcatcatc caggggcggc
     2521 gtggtccgtt tgtccggacc ataaatcagc cggtgcagac tctggggtac gatggcaatc
     2581 tccggttttt caccattgaa tgacacccac ccgggcgtca gattatggaa gtagtagata
     2641 ttcggatgac acacgcccca ggatacggcg tgccgggtga agccggtcag ccatttctgg
     2701 tagctttcgt ggagcagctc gtaacgggtc tgatactgtg gtttcatttt catattcctc
     2761 acgggggata acaggcagca gaacgccgcc ggcaggccgg agactgcgtc tgttgcagat
     2821 gaacggttaa gaattacgga cgggactgtg caatacgcgc ctgcagccag gcttccactt
     2881 cgcttttcag ccatcgggag ctgcggccca ttttgatggg cgcaggaaag tcaccatcct
     2941 tgatgagttt gtaaaaccac ttatcggtca ggccggtcag ttgagtgata aatgccatgt
     3001 cgaccatctg gtcatccatc agtgaaactg gggtgagcat gagaatgttc ctccggatgt
     3061 tgttaaacca gccggtgtgg tcatgaaaac aacgcaccac cttcaccggc ataacaaccg
     3121 gggagaaacg taaaaaataa tcgggaatgt gaccgccagg agataaacgg ggaggttacg
     3181 gtggctgagc cgtaccggta tgccgggtgg ttgttacgaa acgatgcttc cgcacgcgcg
     3241 cgcgataaag aagggatgat tttcgtgagg aagaggggaa aactggcgtt ctgataattg
     3301 cagagttatt tacggggcgg ttacgatggg gcagaccgtg tccggtgggc aggcgacagg
     3361 tatcactggt gggataagac tgtctgattg gtattataaa tatataagag agatcaaaca
     3421 catgag
//
LOCUS       DAOTRK010000260         3404 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.263, whole genome shotgun sequence.
ACCESSION   DAOTRK010000260 DAOTRK010000000
VERSION     DAOTRK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.263"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..626)
                     /gene="gloC"
                     /locus_tag="QIE41_004633"
     CDS             complement(<1..626)
                     /gene="gloC"
                     /locus_tag="QIE41_004633"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDS3533408.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFL"
     gene            complement(653..1201)
                     /locus_tag="QIE41_004634"
     CDS             complement(653..1201)
                     /locus_tag="QIE41_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDS3533409.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1382..3229)
                     /gene="ldtD"
                     /locus_tag="QIE41_004635"
     CDS             complement(1382..3229)
                     /gene="ldtD"
                     /locus_tag="QIE41_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDS3533410.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
BASE COUNT          805 a          906 c          847 g          846 t
ORIGIN      
        1 tgcaggaatg gattatgcag gcgctcataa ccaagtgtgg ataatggtcc gtgacccgga
       61 ataaatgtca cgtcatcccc cagtggcagc aacttatctt tgattgaaga aatcagttga
      121 ttatgatcgc cacgcgggaa gtcactgcgc cctactccgc ctttgaaaat aacgtcgcca
      181 gaaatcagca gctttgcccg atcatcaaaa aacacgacat gacccggcgt atgcccaggg
      241 caatgtaaca cctgtaaagc cacattccct atgctgatgg tatcgccttc gttcagccaa
      301 cgatctggcg tcagcggctg gcactcttcc agaccaaaca tacgactttg cgcaggcaag
      361 ccttgcagcc agaactcatc ttctttttcc gggccaaaaa ccggcacgcc gtaatgttgc
      421 gccagttccg ccgctgcgcc aacgtggtcc agatgaccat gcgtcagcag gatctgcatc
      481 agtgtcaggc cgctggcgtc aacttcctgt ttgatttttt ccgcatcgcc gccaggatcg
      541 accagtgcgg ccagacgggt ttgttcacac cagatcaatg aacagttctg ggagaatgcg
      601 gtgaccggaa taatacgata gttcatactg cccctgtttc gttaagcaat tactaccagt
      661 gccgtgctgg cccggtatca atatgcacaa agttactacg tgggtaatat cctacaccac
      721 ctgcgcgcat agataacgcg gctttgcgaa tattgcttaa cgcgatacct tcaatatgga
      781 aatccatcgc ctgccctttc gtgtgatagc ttttcttcgc tactccacgg ctgcgggcgc
      841 gtagttcatt gttggtatca atggagcgat aaccggaaat gagttgcacc ggtttgcggg
      901 tgcctaacaa cccttgcagg cgatacaact gatcgaataa tcctgggtcg atggacttta
      961 ttttgttcgc gcgataatcg cggaaaaaat ggttaagttt tgccaattct tcctgaatat
     1021 agcctctgcc atcgaaaaac tccgctttga ttgactctcc ggtatgaaga ttattgagtg
     1081 tcaaaatacg cgggcgtggg gtagagagtg ttgcaaacgc aggggtcggc aggatggcgg
     1141 caccgagtgc aacgccacca agcgccagca gtttgcggcg attagcgtcg aatttgtcca
     1201 tgataatcaa gtctacaggt caatgttatc gtttatatgc acttctggcg cacaagaggc
     1261 accttaaccg tcagtaaagc tgacgtcaag atgactcaac cccagcagtg acgggggctg
     1321 cagagaatcg cccccgatca tgctaattat tacgacaact gatttccccg aactgcttca
     1381 tttacctgat taattgttcc gctttcgata cgatttgcga gctggatcgc gcaggcagat
     1441 cataattgta aatatctgta cgatactggg tacgaccatc tgcaccaaca aaggccgtca
     1501 ggtagtagag attcaccgga atcgactgcc gaatattgac gtaacgcgta tcgccctgct
     1561 tcagcgcatc agaaatacgt ttgtcattcc atcctgcatc ctgcaacagc atattcgcca
     1621 gatcggaagc tttattcact cgtacacagc ctgagctcaa tgcgcgtgta tcacgcttga
     1681 acagattgtg gttcggcgtg tcatgcaaat aaatggcctc tgaactcggc atattgaatt
     1741 tatagcgccc cagcgagttc cgtgggcctg gagcctgctg gaagcgaaac ggtaaattcg
     1801 aggccgtgat tgtagaccag tcaacctgcc atgggtcaat cgcttctctg ctgttccagc
     1861 cgcgcatcac cgtatagcca tggctttcga ggtatcccgg atcgttgcgc actttcggca
     1921 gaatatcttt gcgtgccaga gttggtggta cgttccacgg cgggtttacc actacgttgt
     1981 taagtgcact gctcatcatc ggcgttttgc gatcggggcg accgacaatg actcgcgaat
     2041 ccagtacctg attgccgttc tgatagtaga ccagcgaata ggctggaatg ttaaccatga
     2101 tcccggtaga aagctctgtt ggtagcaagc gcaatcgctg gatgttgagt gccaacacgc
     2161 cagcacgctg ggcgggcgtt acgtttaacc agtcacgcgt tgccgggcca atagcgccat
     2221 ctgctcccaa tccttgccat gcctgaaaac gtttaacggc ctccaccagt tcattatcgt
     2281 aggcggcacg aacggcaggc gcaggtttac tacgagacgt cgtttgcttg tccatcggct
     2341 tagtttctgc tgtttcaaca gtaacagcgg atgggctgac tactgcgtca gttggcgtgt
     2401 catcgccagg gagagtaatt tttggccccc cgtccaacat gcctgtgcgt tgcaatattt
     2461 cgcgcaacgc cggtacgtcg ttactccact gccctgggcg caacgttgct ttaccggtca
     2521 gttgaggcca cggtttggtg tcactgagta aggccagtaa cgattcatgc atcgccgcat
     2581 attgcggatg ctgcggtgcc agtcctgcaa caaacgtcgg caattgacct ttatccagcg
     2641 ccagctgcca ttggttaatc accgaaagcg gcggcgttgc aagcgcataa ggtttactgc
     2701 tatatagcca gcgagtgcct ttgaccggaa tatttgcaat gaaatggaga tagcccatca
     2761 tcgcatcaga gagcacgacg tcgcgtgcca ttccgttaac accaggatcg gtcagtaact
     2821 ctacccattt attaaactgc ggctggaaac cggcaatcgc cacctcggcc agctgttgct
     2881 ggaaggcttt aacagcatca cggttttccc acatgggttg catatcgcgt gcggcataca
     2941 acagttgaag ctggttaaga taaaccggct tgtaacctgc gggtaattga gattcgattt
     3001 gcgtgcgggc tttttctgcc gcaccttcag gcaatggctg gatccccgcc attattgccg
     3061 tggcttgcgc ctgcggcagt gcctcgccct gttcactgac agccaccggg ctgtcgccag
     3121 ggattacttc aggctcatcg gcctgcgcat tgaacagtgg agcgaatgtt acggccaggc
     3181 acaaactgat tgccgacagc cgacgaccac acatcatatt aagcaacatc ccttgccccc
     3241 tgtttttatc atactctgaa tagccaggct gttattttac gacttgcctg tacaacgcca
     3301 atactcacga aagtatgacg agcaagcctg ttactcatca ggcttatctt cagtatataa
     3361 acgccgatac gtttttacat aacctaatgt aaagaagttt aaag
//
LOCUS       DAOTRK010000261         3337 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.264, whole genome shotgun sequence.
ACCESSION   DAOTRK010000261 DAOTRK010000000
VERSION     DAOTRK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.264"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            347..1663
                     /gene="shiA"
                     /locus_tag="QIE41_004636"
     CDS             347..1663
                     /gene="shiA"
                     /locus_tag="QIE41_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDS3533411.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1765..3219
                     /gene="amn"
                     /locus_tag="QIE41_004637"
     CDS             1765..3219
                     /gene="amn"
                     /locus_tag="QIE41_004637"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDS3533412.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKYQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          772 a          807 c          806 g          952 t
ORIGIN      
        1 gcaatacgtc tcaacaatga tactaaaaac ataactaacc tcgcctccct actggttata
       61 atgcaatgca gtctatcaga atcatcaggg tgcaattttg tgcatatgcg gacttttatg
      121 tttcatatct cgaacctgtg ggccctctgc ttaatcctta aacaacacca gcaactcctg
      181 cgctttcatc ttccatcgaa tttttcatgt tgccgttaat cagccataaa atcatttgca
      241 gattcgctct gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt
      301 accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct
      361 catctccact cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt
      421 aggcagtttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcattaccgc
      481 cgcactggtg tttaatcgcg agtttttccc gcaggtaagc ccggcgatgg gaacgctcgc
      541 cgcatttgcc acctttggtg tcggcttcct tttccgaccg ctcggcggtg tcattttcgg
      601 tcactttggt gaccggctgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg
      661 catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc
      721 tattttgctg gtgacactgc gtgccattca gggatttgcc gtcggcggcg aatggggagg
      781 cgcagcgttg ctttccgttg aaagtgcgcc gaaaaataaa aaagcctttt acagtagcgg
      841 tgtacaagtt ggctacggag taggtttact gctttcaacc ggactggttt cattgatcag
      901 tatgatgacg actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag
      961 catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt
     1021 tgaacaacag caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct
     1081 gttacgacat cccggtgctt tcctgaagat tattgcacta cgactgtgcg agttgctgac
     1141 gatgtacatt gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg
     1201 cgaacttttc cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg
     1261 ttttgcctgg cttgccgatc gttttggtcg gcgcagggtt tatatcacag gcgcgttgat
     1321 cggaacgttg agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat
     1381 agttttcttc tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca
     1441 accgatgttt accgaaatgt ttggtgccag ttatcgctac agtggtgctg gagtcggtta
     1501 tcaggttgcc agtgtggttg gcggtggatt tacacctttt attgccgctg cactcatcac
     1561 ttactttgcc gggaactggc atagcgtcgc catttatttg ctggctggat gcctgatttc
     1621 tgcaatgacc gctttattga tgaaagacaa tcaacgctcg tgatagccta gcgaagatca
     1681 tccgatcttc gccttatact tttgtttcac attcctgtga catactatcg gatgtgcggt
     1741 aattgtatgg aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag
     1801 gcactggata aactcgacgc gctgtatgag caatctgtag ttgctttacg caacgccatt
     1861 ggcaaatata ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt
     1921 gtctatccat cactgaccgt aacctgggac ggtagcacaa ctaatccccc caaaacgcgc
     1981 gcatttggtc gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc
     2041 tttcgttcgt atctcaatga acaacttacg ttgctgtatc aggattatgg tgcgcatatc
     2101 tctgtgcaac cctcgcagca tgaaatccct tatccttatg tcattgatgg ctctgaattg
     2161 acacttgatc gctcaatgag cgctggttta actcgctact tcccgacaac agaactggcg
     2221 caaatcggcg atgaaactgc agacggcatt tatcatccaa cggaattctc cccgttatcg
     2281 cattttgatg ctcgccgggt tgatttttcc ctcgcacggt tgcgccatta taccggtacg
     2341 ccagtagaac attttcaacc gttcgtcttg tttaccaact acacacgtta tgtggatgaa
     2401 ttcgttcgtt ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct
     2461 tgtgctggcg ggaactggat cactgccgaa accgaagcgc cagaagaagc catttccgac
     2521 cttgcatgga aaaaatacca gatgccagca tggcatttaa ttaccgctga tggtcagggt
     2581 attactctgg tgaatattgg cgtgggaccg tccaatgcta aaaccatctg cgatcatctg
     2641 gcagtgctac gcccggatgt ctggttgatg attggtcact gtggcggatt acgtgaaagc
     2701 caggccattg gagattatgt ccttgcacac gcttatttac gcgatgacca cgttcttgat
     2761 gcggttctgc cgcccgatat tcctattccg agcattgctg aagtgcaacg tgcgctttat
     2821 gacgctacca agttagtcag cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca
     2881 ggtactgtgg taaccacaga tgacaggaac tgggagttac gttactcagc ttctgcactt
     2941 cgttttaact taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa
     3001 ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttacat
     3061 ggtgagatta agcttcccgg ccaggctaac cgtttttatg aaggcgctat ttccgaacac
     3121 ctgcaaattg gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattca
     3181 cgtaaattac gaacctttaa tgagccgccg ttccgataat aagttatgaa ccttcttatc
     3241 agaaatagat gtgaaggatt aagtaagaca tctggcaaat agcctgcaat aaacgcgggc
     3301 tgtttgcttc cttgggcaga tgcgagtttt attatcg
//
LOCUS       DAOTRK010000262         3335 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.265, whole genome shotgun sequence.
ACCESSION   DAOTRK010000262 DAOTRK010000000
VERSION     DAOTRK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.265"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            96..1793
                     /gene="maeA"
                     /locus_tag="QIE41_004638"
     CDS             96..1793
                     /gene="maeA"
                     /locus_tag="QIE41_004638"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDS3533413.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEETARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1927..2937
                     /gene="adhP"
                     /locus_tag="QIE41_004639"
     CDS             1927..2937
                     /gene="adhP"
                     /locus_tag="QIE41_004639"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDS3533414.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRSEDA
                     AKIVQEKTSGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3054..>3335
                     /locus_tag="QIE41_004640"
     CDS             3054..>3335
                     /locus_tag="QIE41_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDS3533415.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
BASE COUNT          843 a          816 c          917 g          759 t
ORIGIN      
        1 ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac gataaaagcg
       61 tgccagggtt ggatattcaa aaaagagtga gtgacatgga accaaaaaca aaaaaacagc
      121 gttcgcttta tattccttac gctggccctg tactgctgga atttccgttg ttgaataaag
      181 gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta ctgccggaag
      241 tggtcgaaac catcgaagag caagcggaac gagcatggat ccagtatcag ggattcaaaa
      301 ccgaaatcga caaacacatc tacctgcgta acatccagga cactaacgaa accctcttct
      361 accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc ccaaccgttg
      421 gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg tttatctctt
      481 accagaaccg ccacaatatg gacgatattc tgcaaaacgt gccgaaccat aatattaaag
      541 tgattgtggt gactgacggc gaacgtattc tggggcttgg tgaccagggc atcggcggga
      601 tgggcattcc gatcggtaaa ctgtcgctct ataccgcttg tggcggcatc agcccagcgt
      661 atacccttcc ggtggtgctg gatgtcggga cgaataacca acagctgctt aacgatccgc
      721 tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa ttcgttgatg
      781 aatttatcca ggctgtgaaa caacgctggc cggacgtact gttgcagttt gaagacttcg
      841 cacaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt tcttttaacg
      901 atgacattca gggcaccgcg gcggtaacgg tcggcacact gatcgcagcc agccgcgcgg
      961 caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca gcgggatgcg
     1021 gcattgccga aatgatcatt gcccagactc agcgcgaagg attaagcgag gaaacggcgc
     1081 ggcagaaagt ctttatggtc gatcgctttg gcctgctgac cgacaagatg ccgaacctgc
     1141 tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg gataccgaca
     1201 gcgatgtgct gtcactgctg gatgtggtgc gtaatgtaaa accagatatt ctgattggcg
     1261 tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat aaacactgtc
     1321 cgcgtccgat cgtgatgccg ttgtctaacc cgacttcacg cgtggaagcc acaccgcagg
     1381 acattatcgc ctggactgaa ggtaacgcgc tggtcgccac tggcagtccg tttaacccag
     1441 tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt attttcccgg
     1501 gcatcgggct gggcgttatt gcttccggcg cgtcacgtat caccgatgag atgctgatgt
     1561 cggcaagtga aacgcttgct cagtattcgc cactggtgct gaacggcgaa ggtctggtac
     1621 tgccggaact gaaagatatt cagaaagtct cccgcgcaat tgcctttgcg gttggcaaaa
     1681 tggcgcagca gcaaggcgtg gcggtgaaaa cctccgccga agctctgcaa caggccattg
     1741 atgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc taagcctgcg
     1801 cccggtagtc aaggctaccg ggcaatttcc ctctcccttt ttcagatctc atccatactg
     1861 ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat ccgaaaagga
     1921 ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac ggataaaaca
     1981 ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg tgtatgtcat
     2041 accgatcttc atgtgaagaa tggcgatttt ggtgacaaaa ccggcgtaat tctgggccat
     2101 gaaggcatcg gtgtggtggc agaagttggc ccaggtgtca cctcattaaa accaggcgat
     2161 cgtgccagcg tggcgtggtt ttacgaagga tgcggtcatt gcgaatactg taacagtggt
     2221 aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg agggatggcg
     2281 gaagagtgca tcgtggtcgc cgattacgcg gtaaaagtgc cagatggtct ggactcggcg
     2341 gcggccagca gcattacctg tgcgggggtc accacctaca aagccgttaa gctgtcaaaa
     2401 attcgtccag ggcagtggat tgctatctac ggtcttggcg gtctgggtaa cctcgccctg
     2461 caatacgcga agaatgtctt taacgccaaa gtgatcgcca ttgatgtcaa tgatgagcag
     2521 ttaaaactgg caaccgaaat gggcgcagat ttagcgatta actcacgcag cgaagacgcc
     2581 gccaaaattg tgcaggagaa aactagtggc gctcacgctg cggtggtaac agcggtagct
     2641 aaagctgcgt ttaactcggc agttgatgct gtccgtgcag gtgggcgtgt tgtggcagtc
     2701 ggtctgccgc cggagtctat gagcctggat atcccacgcc ttgtgctgga tggcattgaa
     2761 gtggtcggtt ctctggtcgg cacgcgccag gatctaaccg aagccttcca gtttgccgcc
     2821 gaaggcaaag tggtgccgaa agtcgccctg cgtccgttag cggacatcaa caccatcttt
     2881 accgagatgg aagaaggcaa aatccgtggc cgtatggtga ttgatttccg ccgctaagag
     2941 gcctttgctg cgactgccat gttcgggtcg cagcatcgca cactctccaa catgaaatgg
     3001 ctaaatggat tattgttgcg taggaaggaa cgctactgag agtaaagaca ctaatgaaaa
     3061 tggccaatca tccccgcccg ggggacatta ttcaggaatc actggacgaa cttaatgtca
     3121 gcctgcgcga gtttgccaga gcaatggaaa ttgcgccctc aacggcaagc cgattgctga
     3181 ccggaaaagc agctttgaca ccagaaatgg cgattaaact ttccgtggtg atcggcagtt
     3241 cgccgcaaat gtggctgaat ctgcaaaacg cctggagtct ggcagaggca gaaaaaacgg
     3301 tggatgtgtc gcgattacgc cgtctggtta cgcaa
//
LOCUS       DAOTRK010000263         3280 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.266, whole genome shotgun sequence.
ACCESSION   DAOTRK010000263 DAOTRK010000000
VERSION     DAOTRK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.266"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..363)
                     /locus_tag="QIE41_004641"
     CDS             complement(<1..363)
                     /locus_tag="QIE41_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDS3533416.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGV"
     gene            complement(418..1269)
                     /gene="manZ"
                     /locus_tag="QIE41_004642"
     CDS             complement(418..1269)
                     /gene="manZ"
                     /locus_tag="QIE41_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDS3533417.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1282..2082)
                     /gene="manY"
                     /locus_tag="QIE41_004643"
     CDS             complement(1282..2082)
                     /gene="manY"
                     /locus_tag="QIE41_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDS3533418.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAPGNNDLDNELD"
     gene            complement(2145..3116)
                     /gene="manX"
                     /locus_tag="QIE41_004644"
     CDS             complement(2145..3116)
                     /gene="manX"
                     /locus_tag="QIE41_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDS3533419.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
BASE COUNT          863 a          841 c          795 g          781 t
ORIGIN      
        1 cacgccatct tccgacaagt tcatcgcttt gattcggcta tattcaatcc agacattggc
       61 gaagaaaaaa cctttttgtt taaaaatgat cttcggaacg cggatccaga atatataaaa
      121 acccatcaga gccagtgcac ttaataacca tgttgttatt agcgcgccat gattcgtgac
      181 gttgttatag ataagaatga caatcaatcc gacgaagata acgctatcga tgcgaccacg
      241 ccggagcaaa ggaattgcca gcagggtggg gccgttacgg cggggcatga tgaactgatc
      301 gtagatcgcg aaggccagga gtgcggcgat gaaaagaatc agtaccaggt ccgtgattgt
      361 cattaatcct ccagataaaa aaacggggcc aaaaggcccc ggtagtgtac aacagtctta
      421 cagtcccagc aggccgcaag cgtaaccagc gataccgatg acgaagaagc caacgatgat
      481 ccacagcggg ttaacttttt tgcgcagtag ccacatacaa gcaaaggtca gcagcagtgg
      541 taccaggcct ggcattaact ggtccagaat agtctggaca gtggtaacgt gttctttgcc
      601 cgtctggtca gtaatgcgag agacaaccag cgggatgttg acatgcgtcc acttgttaac
      661 taatgccccc atgacaaaca ggccgaggat agacgcccct tccgtcagtt tttgcaggaa
      721 gccaccaccc atatctttaa cgatatcgat acctttggag taaccatacg ctacgccgta
      781 gtaacgggtt gccagacgca ccaggttaaa caagatgaag aacagcagcg gacctaacag
      841 gctgccgctc attgcgatac cggcacccag cgctgcaaat accggacgta cggttcccca
      901 gaagatcggg tcgcctacac cagccagcgg ccccatcaaa ccgactttga taccgttgat
      961 agcaccgtca tcgatctctg cgccattagc acgctgttct tccagcgcca gggttacgcc
     1021 gagaatcggc gcagccacga acggctgggt gttaaagaac tccaggtgac ggcgaatagc
     1081 ttgtttacga gcttcgttgt tctcagggta gaggcgacga attgccggta ccatagagaa
     1141 gcagaaaccc agtgcctgca tacgttcgaa gttccatgaa ccctggaaga ggttagaacg
     1201 caggaagacg ccacgaatat cactttgagt gagttttttc tcggtggtag tttgagttgt
     1261 atcaaccatt tcgctcacct gttagtccag ttcgttatcg agatcgttgt taccaggtgc
     1321 ctgagcaggc gcaccggcta cgcggttgta tttcgggcta agttggatgt agagcactgc
     1381 cataacagta ccaatcacac ccagagcaac caggttaaag ttggtgaatg ctgcggttac
     1441 gaagccgagg tagaagaacg gcatcaggta gccagcacgc atcatgttga taaccatcgc
     1501 ataaccaacc accacgatca tgccaccggc gatattcaga ccattggtca ccacttccgg
     1561 aatcgcgttc agcatgttct gtacttcgct ggtaccaaca gacagcgcaa cgatgacggc
     1621 cggaatagcc acacgcattg cttgcaggaa cagagaagaa acgtggatcc aggaaatcgc
     1681 tgtcaggttg ccgttatcag cagccttatc cgcagcgtgc tggaaagcaa cggtaatagt
     1741 acgaacgatg atggtcagta cctggcccgc agcggccaga gggattgcca gtgcgatacc
     1801 tgcaccaatg ctctgatgac ctgcgataac cagaatagta gaaatgatgg aagccagagc
     1861 ggcgtcaggc gcaactgcag caccgatgtt catccagccc agcgcgatca tttccagcgt
     1921 accaccgata ataataccgg ttttcatatc gccaagaacg atacccacca gggtacacgc
     1981 gattagcgga cggtgaaact gaaattcatc gaggattgat cccatacctg cgatacaggc
     2041 tacgataaat accagcacaa tttgaagagt ggtaatctcc attgtacttc tcctgtttac
     2101 gtaagactta agtgtgaaaa ctgagtgata atcaacacaa tacgttactt atcgattttg
     2161 ctgatcagat ccatcatttt cagtttcgga tcggtggaaa ccttacggac ttccagctca
     2221 ataccgcgcg cattcagttt cttgaacgcc tcgatatctt tttcatcaac cgaaaccgcg
     2281 ttattcacct gggttttacc ctgacggaat gccataccac cgacgttaac agaggtgatt
     2341 ttcacgccgc cttcaacgag acgctcaaca tctgttgggt tggtaaataa cagcattacg
     2401 cgttcgccag catatttcgg gttgttgtag acgcgaatca ttttggcaac atcaactacg
     2461 tgtgctgtta cgcccggagg tgcaacctgg gtgagcagtg tcttacgaac ggtatccgca
     2521 gccacttcat cactaacaac aataatacgg gagacattgg tttctttggt ccagcgggtg
     2581 gcgacctgac cgtgaatcag acggtcgtcg atacgcgcaa ggccaataac catgtagtcg
     2641 tttggtccca ttggttttgc cggagttgga gctgcttttg gtgctgcggc agcgggtgct
     2701 ggcgcggctt tttcaaccgg tttggctttc agtgctttca cgccttcacg gcctgtttct
     2761 actgccagcg ccaccagttc atcaaagctt gggtcatcat cacgggccat taacgtttcc
     2821 acgagcattg gaatgttaac gcctgcaatg acttcataat gctctttgtc gacgacaatg
     2881 cggctggcag cattgaacgg gctgcctccc catgtatcaa cgagaaacag cacgccttta
     2941 gtggtgtcga gttttgccaa ctgagcgttg tacttttcaa tcagcgtttc ggcattttca
     3001 cctggaacga aatcgatcca gccgacgttt tcctgctcgc ctaacagcat ttctgccgtt
     3061 ttaagcaact gctctgcagc ccaaccatgt gtgcctataa caatagcaat ggtcacttgc
     3121 tacctccttt attatcgtta acacctcaac gtgccagatg tatttttgaa tcgtcgtcca
     3181 caatcgaatc gattcagata agggctacaa ccaaaaaccc ctgcgtttcg cgatttattt
     3241 tagatatcga aaaaattatt ttatgtgatg aagatccgta
//
LOCUS       DAOTRK010000264         3260 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.267, whole genome shotgun sequence.
ACCESSION   DAOTRK010000264 DAOTRK010000000
VERSION     DAOTRK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.267"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(73..780)
                     /locus_tag="QIE41_004645"
     CDS             complement(73..780)
                     /locus_tag="QIE41_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533420.1"
                     /translation="MRKKIEMSLINSPANGVVIKRIISDGLKEIVSLKEQILLETTAK
                     IQSIEEKREEKVIQGYYDGYAKGIIDVMDSFILLISLLSSELEKNRINMINDLKSILL
                     KSSEEVEVFIKIFESWVTKLPSISGPLNLYIPTSFKDKSIEVESYFVDKSIWNVHISY
                     HDDKRFIFFTDQFIAEFSPQEFVDNCEQYLISNHCFSPDKVNEICEHARHYLVERMCE
                     IDSLAMNNSDLTNPEDL"
     gene            complement(707..1303)
                     /locus_tag="QIE41_004646"
     CDS             complement(707..1303)
                     /locus_tag="QIE41_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HDS3533421.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNARISNPVLRSITNEMILLQ
                     YNLSVEHFSLNSSLIYYINNWKLFPLICLLSGCHFYRERFAERGFFYKIPEVLRNYLS
                     AIPVEINEKARYKPGIVNYQNIITCGFSTLLPYVRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKLSCKWCCDQKNNF"
     gene            complement(1319..2053)
                     /locus_tag="QIE41_004647"
     CDS             complement(1319..2053)
                     /locus_tag="QIE41_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HDS3533422.1"
                     /translation="MKYISLFLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEINEPIFAYDKTMPVSILLA
                     LMSIATCWLLWKYRAILTNLIRLKNK"
     gene            complement(2050..2382)
                     /gene="sctI"
                     /locus_tag="QIE41_004648"
     CDS             complement(2050..2382)
                     /gene="sctI"
                     /locus_tag="QIE41_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDS3533423.1"
                     /translation="MSVSNMPPIDRAEQSAAREIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            complement(2402..2641)
                     /locus_tag="QIE41_004649"
     CDS             complement(2402..2641)
                     /locus_tag="QIE41_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HDS3533424.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            complement(2655..>3260)
                     /locus_tag="QIE41_004650"
     CDS             complement(2655..>3260)
                     /locus_tag="QIE41_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDS3533425.1"
                     /translation="RDHVIYINTNSLDEAVWVKQALEKNQPGKPVRVINPDDESIRIF
                     SWLADNFPDLQYFKLQLLDASNPRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISI
                     AVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQ
                     WGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
BASE COUNT         1013 a          612 c          517 g         1118 t
ORIGIN      
        1 gttgaaaaat cattaggcgt ttaaaatatt agaattagat tatattcaag ttattaaacc
       61 ggagacagtg atctataagt cctctggatt cgtcaaatca gaattattca tagccaaaga
      121 atctatttca cacattcttt caactaaata atgtctggca tgttcacaga tttcattaac
      181 cttgtcaggg gaaaaacagt gattgctaat taaatattgc tcacaattat ccacaaattc
      241 ctgcggacta aattcagcaa taaattgatc tgtaaaaaaa ataaaacgtt tgtcatcatg
      301 atatgaaata tggacattcc atattgactt gtcgacaaaa tatgactcta cttcaataga
      361 cttatctttg aaactggtag gtatatagag atttaaaggg cctgatattg acggtaattt
      421 tgttacccaa ctttcaaaaa ttttgataaa aacttcaact tcctctgaag attttaacaa
      481 tatgctcttc agatcgttta tcatgtttat tctgtttttt tctaattctg agctcagcaa
      541 acttatcaag agtataaaac tatccatcac atcaataatg cctttcgcat agccatcata
      601 ataaccttga ataacttttt cttctctctt ttcctcaata ctctgaattt ttgcggtggt
      661 ttcaagaagg atctgttctt ttagagaaac tatttctttc aatccatcag aaattattct
      721 tttgatcaca acaccatttg caggagagtt tatcaagctc atctctattt tttttcgcat
      781 aaaatgtaat tctctccaaa agagtagacg caagtggtaa cggcaattgt atgtggtcaa
      841 caaaatcagg aaataataaa ttaaatcgtt gctgcattgc caacggttgt tgccttacat
      901 agggtaacaa cgttgaaaaa ccacatgtga tgatattctg atagttaacg ataccaggtt
      961 tatatcgtgc cttttcattt atttccaccg gtatcgctga taaataattc cttagcactt
     1021 caggaatctt gtaaaaaaat ccgcgttctg caaacctttc tcgataaaag tgacaaccac
     1081 tgagtaaaca gatcaatgga aataatttcc aattgtttat gtagtaaata agtgaactat
     1141 tcagactgaa atgctcaact gaaagattgt actgtaggag tatcatttca ttggtaatag
     1201 atctaagtac cggattgcta atacgtgcat tattcaaact tactcgttga ggatgaatat
     1261 aactaattgg atcatatata atctttctta gggcaagatt cataacgttc tcatatgtct
     1321 acttattttt taacctgatt aaattagtta gaatagccct gtacttccat aacagccagc
     1381 aagtcgcaat ggacattaat gcaagtaaga tagaaaccgg catagtctta tcataagcaa
     1441 aaataggttc gttaatttca tatgttggtg cttgttcaat aatatctctt cgttttgaca
     1501 atacgactga aatattctca tattgtacgc ttgcaaagct attaacaata aatctcttaa
     1561 tgtcatttat ttttatttct ggattgatat ctttttcata tacagcaagt acagaaatat
     1621 gaattggtaa agcagttttg ccactatcac ctgtatcaac atcgtaacta acatgtactc
     1681 ttgaagaaac aatgccatcc ataattttga gagattgctc taaacgctgt tcaatagcag
     1741 aatacaatct tgctttttcc gctcgtggag aggaaacgag tgcatctgca gggaacatct
     1801 gcgatatttg aatatcaggc ttacccggga gattgtagat ttttagccaa tccaccgcag
     1861 aagcaaaatc cgttggttct acatagattg aaaatccagt tttgccttga tccttcttct
     1921 cagcattaat attatgtctt tgtaaaacag caaggacatc attagcttgc tgttgatcaa
     1981 gatggttcag taattcctgc tgcttgcaac cgcacaacag caggataaac aagaataatg
     2041 atatatattt catgagcgag tcaacgtctc aactgcagcg acaatttttc ttgctaatgt
     2101 tcccaccaat gaaacctcga cattataatt aagcacatga tcctgcaaga ctaataattt
     2161 ttcaggagtt aacccctttt tatcagacct taatgcttgt aacgcatttt gctgccaatt
     2221 ttcagtttgt accgcatagt tggcaaaagc ggagatcatt ttatcatccg ggttatcaag
     2281 attgagaacc cggtcattta aatcaataac tttcgcttgc tgtatttcac gagcagctga
     2341 ttgctcagct ctatcaatag gtggcatatt gcttactgac ataataaaat ccttatccag
     2401 tttagcggaa gttttgtatg attgccgaat caagatcttt atacgctttt acaacgttag
     2461 attgcgcatt tcgatataat gtatactcag ctaatgcact ctgatattcg gcaaggaatt
     2521 tcgggtcgga aggattggtt gctaaatcct ccaacgcctt ttcaacttgt tggttcagat
     2581 catcaacgcc ctgatcaaat tgtttgctga tatccataat ataaccattc caatctgcca
     2641 taagtcctcc tcaattattc ctgaaatgtc cacttgctac cttgagactt ctcgaatcgg
     2701 ttatctccgt agctaaatga actattattt gtatcctgat ttttcaaatt tacattaaat
     2761 cttacatatt gtttacccca ttgtttataa tattcgtcga catagttatt aattttattt
     2821 aattcactgt cgcttaacgt accaatgata ttgaaatata cattgttagc tgttttatat
     2881 ttttcataag aaagacctat tgccgacaat gtttcaattg cctgactttc caatacatta
     2941 tcatctaata ccgcaatact tatattgctg gcatatggca tagtttgcag taatccttta
     3001 ataagattat cgattagttg ctgtgtgata gcattccgtt gcttactcac tgtcagtctt
     3061 ggattactgg catctaatag ctgtagttta aaatattgta gatctgggaa attatcagca
     3121 agccatgaaa aaattcgtat cgattcatca tcaggattta tcacccgtac tggctttcct
     3181 ggttgatttt tttccagtgc ttgtttgacc cagacagctt catccaaact attggtattg
     3241 atatagatga catgatccct
//
LOCUS       DAOTRK010000265         3249 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.268, whole genome shotgun sequence.
ACCESSION   DAOTRK010000265 DAOTRK010000000
VERSION     DAOTRK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.268"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(88..615)
                     /gene="mobB"
                     /locus_tag="QIE41_004651"
     CDS             complement(88..615)
                     /gene="mobB"
                     /locus_tag="QIE41_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDS3533426.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVSLNLDVALLDINDVG
                     GLADFVVEWMQKQNG"
     gene            complement(597..1181)
                     /gene="mobA"
                     /locus_tag="QIE41_004652"
     CDS             complement(597..1181)
                     /gene="mobA"
                     /locus_tag="QIE41_004652"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDS3533427.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQETGEWFLFCPCDT
                     PYIPPDLAARLNHQRKEAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1251..1520
                     /locus_tag="QIE41_004653"
     CDS             1251..1520
                     /locus_tag="QIE41_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDS3533428.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1597..2583
                     /gene="srkA"
                     /locus_tag="QIE41_004654"
     CDS             1597..2583
                     /gene="srkA"
                     /locus_tag="QIE41_004654"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDS3533429.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2600..3226
                     /gene="dsbA"
                     /locus_tag="QIE41_004655"
     CDS             2600..3226
                     /gene="dsbA"
                     /locus_tag="QIE41_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDS3533430.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          821 a          782 c          790 g          856 t
ORIGIN      
        1 aaaggccatt cgaaaggatg gcctttttgc gttggtgaaa aaaatgccgg atgcgacgct
       61 ggcgcgtctt atccagccta ctcttgctta tccgttctgc ttttgcatcc actccaccac
      121 aaaatcagcc agccccccaa catcattaat atccagtaac gcgacatcaa gattaagtga
      181 cacatcactg gctacagcaa taacatgtct gtctatcact aattcttccg gtcgatgtcc
      241 ggctccatcg cgaaacagca caatctttgc gatctcttca tgcttaaacc cttcgaccag
      301 aatcaaatcc agctttgagg tatccattcg acttgcgaga aaatgtagat ccagctcttc
      361 ttcgtctggt gtttccgtca tcaaggccca tcgctgctgg ctggcaacga tggtttgtgc
      421 cgcgccagcc tttcgcagct catagctatc tttgcccggt ttatcaacat ccatatcatg
      481 gtgcgtatgc ttaatcagcc ctggacggat ccctctggcg cataatgccg ggatcagttt
      541 tttcaaaagc gtagtttttc cagtgccgct ccacgcggca aaggcgagta acggtatcat
      601 cgtttttcct gccatcgggc aagctcctct ggcgtattca cgttaataaa tgcatcttta
      661 ttatcgctga aatcaaccgc atgaccgcca gccagacgca taaatgccat tactcggcgt
      721 tctcctgctt gcagatattc cagtaataaa ggctcaattg cgcggtttac cagagcaatc
      781 gtcgggtgat cgcgttcacc gtcgtggacc cacacgacag gtgcctcttt gcgctgatgg
      841 ttaagccggg ctgctaaatc agggggaatg taaggcgtat cgcacggaca aaacaaaaac
      901 cactcccccg tttcctgctg cattactgaa agcattcctg ccagagggcc tgggtaatcc
      961 gccagtgaat cttcaatcac tttcagaccg cttgcctgat agatttcctg atgacgattg
     1021 gcattaacca cgacgtgcga gagctgcgtc ataagcgcgt cagcgacatg ttgccacagc
     1081 ggtttgccgt ttaattcaag caatccttta tctacgccgc ccattcgtct ggctttaccg
     1141 cccgccagca caacgcctgt tatcgtcgtc atcagattca ccgatatcgc ctcttttatt
     1201 gtgggattga ccctgctaac gtgtctgtct caagagtaag gagcattact atgaaatgta
     1261 aacgtctgaa tgaagttatt gaactcctcc agccagcctg gcaaaaagag ccagacctta
     1321 acctgctgca atttttgcag aaactggcga aagagtcagg ttttgacggc gaactggcgg
     1381 atttgacgga tgacattctg atctatcatc tgaaaatgcg tgattccgcg aaagatgcgg
     1441 ttatcccggg tttgcaaaaa gattatgaag aagatttcaa aacggcgctg ttacgcgctc
     1501 gcggcgtaat taaagagtaa aagcttgtaa gcggcgccac caaaatcatc gtgaaatgat
     1561 atccttcatc attcgtaatg ttttccggat gatgggatga acaacagcgc ttttactttc
     1621 cagacactac acccggatac catcatggat gctctatttg agcaagggat ccgggtggat
     1681 tccggtctta ccccgcttaa cagctatgaa aaccgtgtct atcaatttca ggacgaagat
     1741 cgtcgacgtt ttgtcgtcaa attttatcgc cctgaacgtt ggacagccga ccaaatcctc
     1801 gaagaacatc agtttgcgtt gcagctggta aatgatgaag ttccggtcgc agcacctgtg
     1861 gcctttaacg gtcagacttt attgaatcat cagggatttt atttcgctgt ttttccaagc
     1921 gtcggtggtc gccagttcga agctgataat atcgatcaga tggaagcggt tgggcgttat
     1981 ttagggcgta tgcaccagac ggggcgcaaa cagcttttta tccatcgccc gaccatcggt
     2041 ctgaatgaat atctcattga gcctcgcaag ctgtttgagg acgctacact gataccttcc
     2101 gggttgaaag cggcatttct gaaagcgaca gatgagctga tcgccgccgt tacagcacac
     2161 tggcgggaag atttcaccgt tctgcggcta catggagact gccacgctgg gaatattctc
     2221 tggcgcgatg gtccaatgtt tgttgatctg gatgatgcac gtaatggtcc agccgttcag
     2281 gatttgtgga tgttgctcaa tggcgataaa gccgagcagc ggatgcaact ggaaactatt
     2341 attgaagctt atgaagaatt tagcgagttc gacaccgctg aaatcggact gattgaacct
     2401 ttacgcgcca tgcgtttggt ttattatctt gcctggctaa tgcggcgttg ggctgatccc
     2461 gcgttcccga aaaatttccc gtggttaacc ggggaagatt actggctgcg acagacggcg
     2521 acttttatag aacaggcaaa agttctacaa gaaccccctt tgcaattaac acctatgtat
     2581 taatcggaga gagtagatca tgaaaaagat ttggctggcg ctggctggtt tagttttagc
     2641 gtttagcgca tcggcggcgc agtatgaaga tggtaaacag tacactaccc tggaaaaacc
     2701 ggtagctggc gcgccgcaag tgctggagtt tttctctttc ttctgcccgc actgctatca
     2761 gtttgaagaa gttctgcata tttctgataa cgtgaagaaa aaactgccgg aaggcgtgaa
     2821 gatgactaaa taccacgtca acttcatggg tggtgatctg ggcaaagatc tgactcaggc
     2881 atgggcagtg gcgatggcac tgggcgtgga agacaaagtc actgttccgc tgtttgaagg
     2941 cgtacagaaa acccagacta ttcgttcagc atctgatatc cgcgatgtat ttatcaacgc
     3001 aggtattaaa ggtgaagagt acgacgcggc atggaacagc ttcgtggtga aatccctggt
     3061 cgctcagcag gaaaaagctg cagctgacgt gcaattgcgt ggcgttccgg cgatgtttgt
     3121 taacggtaaa tatcagctga atccgcaggg tatggatacc agcaatatgg atgtttttgt
     3181 tcagcagtat gctgatactg tgaaatatct gtccgagaaa aaataattca atgtaaatta
     3241 cataaagtc
//
LOCUS       DAOTRK010000266         3248 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.269, whole genome shotgun sequence.
ACCESSION   DAOTRK010000266 DAOTRK010000000
VERSION     DAOTRK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.269"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..691
                     /locus_tag="QIE41_004656"
     CDS             <1..691
                     /locus_tag="QIE41_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDS3533431.1"
                     /translation="LRGNWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAA
                     MLTGSEQNWRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQA
                     AAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYV
                     GGAGSPAQMRRAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQL
                     RDGGMLSGSGG"
     gene            688..1017
                     /locus_tag="QIE41_004657"
     CDS             688..1017
                     /locus_tag="QIE41_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3533432.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            1017..1715
                     /locus_tag="QIE41_004658"
     CDS             1017..1715
                     /locus_tag="QIE41_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDS3533433.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            1721..2464
                     /locus_tag="QIE41_004659"
     CDS             1721..2464
                     /locus_tag="QIE41_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3533434.1"
                     /translation="MTQTESAILAHARRCVPAESCGLVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            2410..3042
                     /locus_tag="QIE41_004660"
     CDS             2410..3042
                     /locus_tag="QIE41_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDS3533435.1"
                     /translation="MCLHGDLQRFGRRLSLYVNTAAEAIRALSLQVPGFRRQMNEGWY
                     QIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTA
                     GASMVLWGTALSAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTY
                     FSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          683 a          762 c         1117 g          686 t
ORIGIN      
        1 gcttcgcggg aactggatgg caggcctcag gtccggctgg agtgagtggg aagagagtgc
       61 cacggacagt atgtcgcagg taaaaagtgc tgccacgcag acctttgacg gtattgcaca
      121 gaatatggca gcgatgctga ccggcagcga acagaactgg cgtggtttca cccgttctgt
      181 gctgtccatg ctgacagaga tttttctgaa gcaggcgatg gtggggatag tcgggagtat
      241 cggcagcgcc attggcggtg ctttcggtgg tggtgcgtct gcctccacgg ggacggccat
      301 tcaggctgcg gcggcgaact tccatttcgc gaccggggga tttacgggga cgggaggtaa
      361 atatgaacct gcggggattg ttcatcgcgg ggagtttgtc ttcacgaagg aggcgaccag
      421 ccggattggt gtcggcaatc tgtaccgcct gatgcggggc tatgcggaag gtggttatgt
      481 cggcggtgcc ggaagtccgg cgcagatgcg gcgggcggaa ggcattaatt ttaatcagaa
      541 caatcacgtg gtgattcaga acgacggccc caacgggcgg gcagggccgc agctgatgaa
      601 agcggtgtat gagatggccc gcaagggggc acaggatgaa ctccggctgc agttgcgtga
      661 tggcggtatg ttatcaggga gcggtggatg aaaacctttc gctggaaagt gaagccggat
      721 atggaggtga actcgcagcc atcggtgcgt gaagtgcgtt ttggtgacgg gtactcacag
      781 cgtatggcgg cagggctgaa tgctgacctg aaaacataca gggtgacgct ttccgtgacc
      841 cgggaggagg cccggcatct ggaagcgttc ctggcagagc acggtggctg gaaggcattt
      901 ttgtggaagc caccctatgc ataccggcag ataaaggtga cctgtgccgg gtggtctgcg
      961 cgggtcggga tgttgcgcgt tgagttcagc gcggagttta agcaggtggt gaactgatgc
     1021 aggatattcg cgaagaaagt ctgaacgagt cggttaagtc agagcagtca ccgcgggtgg
     1081 tactctggga aatcgacctg acggtacagg gtggtgagcg gtattttttc tgtaatgagc
     1141 tgaatgaaaa aggggagccg gtcacctggc aggggcgtaa gtatgaggca tacccgattg
     1201 acggcagcgg ctttgagatg aacggccggg gcagcagtgc cagaccgtcg ctgacggtgt
     1261 ccaatctgtt cggtctggtc accgggatgg cggaagacct gcagagtctg gtgggggcca
     1321 cggtggtccg ccgccgggtg tatgcccgtt ttctggatgc ggtgaatttc gttgcgggca
     1381 atccggaggc ggacccggag caggagctga gtgaccgctg ggtggtggag cagatgtcgc
     1441 agctgacagc catgacggcc tcgtttgtgc tggctacacc gaccgagacg gacggggcgc
     1501 tgtttcccgg tcgcatcatg ctggcgaaca cctgtatgtg ggattaccgg ggagatgaat
     1561 gcgggtataa cggtcctgcg gtggcggatg agttcgacaa ccccaccacg gatatccgta
     1621 aggacagatg cagcaagtgc atgcgcgggt gtgagatgcg cggcatggtg gctaattttg
     1681 gcggtttcct ttccattaac aaactttcgc agtaaatcca atgacacaga cagaatcagc
     1741 gattctggcg catgcccggc ggtgtgtgcc tgcggagtcg tgcggcttag tggtgagaac
     1801 gccggagggg gagcggtata tcccttgtgt gaatatctct gcagagccgg aggcgtattt
     1861 tcgtattgca ccggaagact ggctgcgggc agagatgcag ggggagattg tggcactggt
     1921 ccacagtcat cccggtgggc tgccctggct gagcgaggct gaccggcggc tgcagataaa
     1981 aagcgcactg ccctggtggc tggtctgccg gggtgacatt cacaaattcc gctgtgtgcc
     2041 acatctgacg ggacggcgct ttgagcacgg ggtgacggac tgttacacgc tgttccggga
     2101 tgcttatcat ctggcgggga ctgagatgcc ggattttcat cgcgaggatg actggtggcg
     2161 tcacggtcag aatctctatc tggataatct ggaggccaca gggctgtatc aggtgccgtt
     2221 gtcagcggcg cagccgggcg atgtgctgct gtgctgtttt ggttcatcgg tgccgaatca
     2281 tgccgccatt tactgtggtg acggcgagct gctgcaccat attcctgaac aactgagtaa
     2341 acgggagagg tattccgaaa aatggcaacg acgaacgcat tctgtctggc gtcaccgcca
     2401 ctggcacgca tctgccttca cggggatttg caacgatttg gccgccgcct cagcctgtat
     2461 gtgaacacgg cagcggaagc catccgtgcc ctgtcgttac aggtgccggg ctttcgccgt
     2521 cagatgaacg aaggctggta ccagatacgt attgccggtg atgacacggc accggaggcg
     2581 gtgtacgccc gtcttcacga acagctgggt gagggaacgg tcatccacat tgtgccgcga
     2641 ctggccggag ccggaaaggg cggactgcag attgtgctgg gggcggcagc catcgtgggc
     2701 tctttcttca cggccggtgc ctcgatggtg ttatggggta cagccctgag tgccggcggt
     2761 ttttctgcca ccacgatgct gttttcactg ggggccagca tgatactggg cggtgtggcc
     2821 cagatgctgg ccccgaaggc aaaaacaccg gattaccgcg caacggataa cggcagacag
     2881 aacacgtact tttcctcgct ggataacatg attgcccagg ggaacccgat gccggtgcct
     2941 tacggggaaa tgctggttgg ctcccgccgt atatcccagg acatcagcac ccgtgatgaa
     3001 ggcgggggcg gaaaggtcgt ggttatcggg cggcaggggt aaaaagaata aaaaaatccc
     3061 gcagtgatcg cggacaggaa ctgcgggaga gttacgaaga ttaagtgtaa ggaattattc
     3121 ttatatcacg acaaaaaaat taacgcagag aaattatacg cgccacagtc agtttgtgaa
     3181 aatgtgaaga tattcagaat ttttatgcca ttaccggttt taaccaacag gattatcggt
     3241 gggcatga
//
LOCUS       DAOTRK010000267         3197 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.270, whole genome shotgun sequence.
ACCESSION   DAOTRK010000267 DAOTRK010000000
VERSION     DAOTRK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.270"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            159..491
                     /gene="nikA"
                     /locus_tag="QIE41_004661"
     CDS             159..491
                     /gene="nikA"
                     /locus_tag="QIE41_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HDS3533436.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            502..>3197
                     /locus_tag="QIE41_004662"
     CDS             502..>3197
                     /locus_tag="QIE41_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDS3533437.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSH
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLAERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWLPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALVDKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
BASE COUNT          829 a          751 c          879 g          738 t
ORIGIN      
        1 cctctgccgt ctgcataaga ctatgatgca caaaaataac aggctataat ggcctgaaaa
       61 acgggacagg atgtgcaatt gtaataccgt cacacgcgac gctattacaa ttgccatctg
      121 gtcagggctt cgccccgaca ccccgtaagg agcctgaagt gagtgatagt gcggtaagaa
      181 agaaaagtga ggtacggcaa aagacagttg tcaggacact cagattctct cctgttgagg
      241 atgaaaccat caggaaaaaa gctgaagatt ccggacttac tgtatctgct tacatccgaa
      301 atgcagcact gaataagcga attaactccc gtacagatga tgcttttctg aaggagttaa
      361 tgagactggg aaggatgcag aagcatctgt ttgttcaggg aaaaagaacc ggtgacaagg
      421 aatacgcaga agtgcttgtt gccataaccg aacttacaaa tacattgcgt aaacagttaa
      481 tggaaggttg aggattctcc ggtgaatgca gtcattccga aaaagagaag ggacggcaag
      541 tcttcgttcg aagacctggt atcctacgtc tctgtccggg atgacatgac cgatgaggaa
      601 ctggatttat cttcttcttc gcaggctgaa caaccgcacc gaagtcgttt cagtcgcctt
      661 gttgattatg cgacacgtct tcgaaatgag tcattcgtgg cgctggttga tgtcatgaag
      721 gacggctgcg aatgggtcaa cttttatggt gtcacctgct ttcacaactg tacatctctt
      781 gaaactgctg ctgcagatat ggagtacatt gcccggcagg ctcactatgc aaaagatgac
      841 actgaccctg tttttcacta catcctttcc tggcagtcac atgaaagccc gcgtccggaa
      901 cagatttatg acagtgtacg tcatacgctg aaatcactcg gccttgccga ccatcagtat
      961 gtctctgccg tgcatactga tacagataat ctgcacgtcc atgtggctgt taaccgggta
     1021 caccctgaaa cgggttatct gaaccggtta tcatggagtc aggaaaaact cagtcgtgcc
     1081 tgccgtgagc ttgaactgaa gcatggtttt gctccggata acggttgctg ggtccatgcg
     1141 ccgggaaatc gtatcgttcg taaaactgcc gttgaacgtg atcgccagaa cgcttggaca
     1201 cgcggaaaaa aacaaacttt tcgcgagtat gttgcgcaga cagcggtcgc tggtttacgc
     1261 agtgaacctg tacatgactg gttatccctg catcgtcgtc ttgctgaaga tggtctgtac
     1321 ctgtctcata tggacggaaa atttctggtg atggatggct gggatcgcaa cagggaaggt
     1381 gtacagcttg actcgtttgg tccctcctgg tgtgcagaaa aactcatgaa aaaaatgggt
     1441 gactacacgc cagtaccaaa agatattttc agccaggtgg aagcaccagg acgctataat
     1501 ccggacttca ttgcagctga tgttcgcccg gaaaaaatcg ctgaaacaga aagtctgcag
     1561 cagtatgcct gtcgtcatct tgcagaacgt ctgccggaaa tggcacggga aggacggctg
     1621 gaaaactgcc aggctattca ccgcacactg gctgaagcgg gtttatggat gcgtgttcag
     1681 catggtcacc tggttatctg tgacggttat gatcataacc agactcccgt tcgtgctgac
     1741 agcgtatggt cactgctgac gctcgacaat gtgaatcaac tcgatggtgg ctggctgcct
     1801 gtaccaacag atattttccg ccaggttacg ccaacagaac gcttccgtgg tcgtcgtatg
     1861 gagagctgtc ctgcgaccga taaagaatgg caccgtatgc gtacaggcac ggggccgcag
     1921 ggggctatca aacgcgaact gttttctgac aaagaaagtc tgtggggata cagcatcagc
     1981 cattgtagcc ctcagattga agaaatgatc acacagggtg agtttacctg gcagcgttgt
     2041 catgagctgt ttgcacaaca gggactgatg ctacagaaac aacatcacgg gcttgttgtc
     2101 gttgatgcct ttaatcatga gcaaacgccg gtgaaggcca gcagcattca tcctgatctg
     2161 acactggggc gtgcagagcc acaggccgga ccttttgtga gtgccccagc agatttgttt
     2221 gacagggtgc aacctgaaag tcgctataac ccggagctgg ctgtcagtga caggtacggg
     2281 gtcagcagta aacgtgaccc aatgctgcgc cggcagcgac gtgaagccag agctgaggcc
     2341 cgtgctgacc tgcgtgcccg ctatctggca tggcgtgaac aatggcgtaa accagatctg
     2401 cgttatgggg aacgttgtcg ggaaattcat caggcatgcc gtctgcggaa gtcacacatt
     2461 cgtgcgcagt acgatgatcc ggcattacgt aagctgcatt atcacattgc agaagttcag
     2521 cgaatgcagg cattgatcag gctgaaagaa gacatcaggg atgagcgaca gaaacttatt
     2581 gctgacggga aatggtatcc accctcttac cgtcagtggg tcgaaattca ggctgctcag
     2641 ggagacaggg ctgctgtatc gcagctgaga ggctgggatt atcgcgatcg ccgtaaagac
     2701 aggtcacgca caacgacgac agaccgctgt gtcgtgcttt gtgaaccggg cggaacgccg
     2761 gtatacggta ataccggtga tctcgaggcc cgtctgcaga agaacgggag tgttcgtttc
     2821 cgtgaccgtc ggacgggaga gtttgtctgt acggattatg gtgacagggt tgttttccgt
     2881 aatcaccatg accgcaatgc gctggtagat aaactggatt tgattgcacc ggtattattc
     2941 gggcgtgatc cacgcatggg ttttgaacca gaaggaaacg acaaacaatt taaccaggtc
     3001 tttgctgaaa tggttgcctg gcataatgtg acaggccgta ccggtcacga agactacagg
     3061 attacgcgac cggacgtgga tcatcaccgt gaaggaagtg aacgctacta ccgcgattat
     3121 atcgcggcga acagcaacga tgacgcatca ctgcctccgc cggagcagga taaacgctgg
     3181 gaaccgcctt ctccagg
//
LOCUS       DAOTRK010000268         3187 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.271, whole genome shotgun sequence.
ACCESSION   DAOTRK010000268 DAOTRK010000000
VERSION     DAOTRK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.271"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..258
                     /locus_tag="QIE41_004663"
     CDS             <1..258
                     /locus_tag="QIE41_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533438.1"
                     /translation="YLQQPKVLHYGVAAKEKFFDKMHNAFRKQFTDGKKDKRVQINVK
                     ISSDAKRTLDRLTRELKTTQQAVLEHLIQNHRLVIQNHRLD"
     gene            complement(255..1187)
                     /locus_tag="QIE41_004664"
     CDS             complement(255..1187)
                     /locus_tag="QIE41_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533439.1"
                     /translation="MTAQQIDALRDIVNKARVTAICKSPAWKYTLRILKRSRVVYRGE
                     RSESFDPEKHFNRYTVRYLYLLSIMALELRSDTRIKVEVGQWYRMTGKRLSLNVPPFM
                     LIPRNIRRKVDGFRQSEGEATKQTAQPFTGSLYEVLSRDNDSAELDAWFAEPPLTRQE
                     VREGRRVTDFNPWAQSSFICRSASPTFELFYQEYKRLGLSVFFDPENRKPFESIKKHF
                     GDKPQLLERLGDVLFFTSLYNQGCLGEFVNALVEKEDIYLKASPGEEKLKAHQKMINY
                     IEEFCNKMTEKYLISAARRHYQKKKIARSRSGES"
     gene            1294..2178
                     /locus_tag="QIE41_004665"
     CDS             1294..2178
                     /locus_tag="QIE41_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDS3533440.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEICQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHHLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRTLYRQLLPELDLIIWILRADERAYAADITMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGDAIGKSFDDAVARFSFPAWMLQLLR
                     KARDRIIHLLVTLWDRLF"
     gene            2297..2974
                     /locus_tag="QIE41_004666"
     CDS             2297..2974
                     /locus_tag="QIE41_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533441.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGNRISINGNLIPTSQMCNQQTGFRAVEVEVGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTPAINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            2980..>3187
                     /locus_tag="QIE41_004667"
     CDS             2980..>3187
                     /locus_tag="QIE41_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDS3533442.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCRNSEADAGKQLNAWL"
BASE COUNT          807 a          849 c          722 g          809 t
ORIGIN      
        1 tacttacagc agccaaaagt acttcactat ggggttgccg caaaagaaaa atttttcgac
       61 aagatgcaca atgcattccg aaaacagttt accgacggaa aaaaagataa gcgggtacaa
      121 atcaacgtta aaatatcatc agatgcaaaa cgtactctgg acagactgac cagagaactg
      181 aaaacaacac aacaggctgt cctggaacat ctgattcaga accacaggct ggtgattcag
      241 aaccacagac tggattaact ttcaccactt ctgctcctgg cgattttctt cttttggtag
      301 tgtctcctgg ctgcggatat cagatacttt tccgtcattt tgttgcagaa ctcttcaatg
      361 tagttaatca ttttctggtg tgctttcagc ttttcttccc cgggggacgc tttcagataa
      421 atgtcctcct tttccacaag cgcattgaca aactccccta aacatccctg gttataaagc
      481 gacgtgaaaa acaacacatc ccccagtctc tccagtaact gtggtttatc cccgaaatgt
      541 ttttttatac tttcaaaagg ttttctattt tccggatcaa aaaaaacact taatcccagt
      601 cgcttatatt cctggtaaaa aagttcaaac gtcggggatg ctgaacggca aataaacgaa
      661 gactgcgccc atggattaaa atctgtgaca cgcctcccct cccgcacttc ctgtcgtgtc
      721 agcggtggtt cagcaaacca ggcgtccagc tcagccgaat cgttatccct gctcaaaacc
      781 tcatacaaac tgcccgtgaa cggttgagcc gtttgttttg ttgcttcgcc ttctgattgt
      841 ctgaatccat ccacttttct ccggatatta cgcggaatca gcataaaagg cgggacatta
      901 agtgataaac gtttacccgt catgcgatac cattgaccta cctcaacctt aatccgtgta
      961 tctgacctta gctcaagtgc cattatgcta agcaggtaca gataacgcac cgtataacga
     1021 ttaaaatgtt tctccggatc aaacgattcg gaccgttctc ctctgtacac gactctgctg
     1081 cgcttcagta ttcgcagagt atatttccag gccggacttt tacaaatggc ggtaactctg
     1141 gccttattaa caatgtcgcg taatgcatca atctgttgtg cagtcatctc ttccctccac
     1201 actttgccac tccagatttt ttcacgccca tattacctga atgataaacc ggattagtgt
     1261 gatccggttc agtgaaatca acaggatacc ggtatgtcat tcagccattc ttccctctcc
     1321 gcacaagtga aatcatatct gacgtttctt cctgaagaaa tatgccagaa aatccttgaa
     1381 catctccacg gtgttattca ttacgagccc gtgattggca ttatgggtaa atccggcacc
     1441 ggcaaaagca gcctgtgtaa tgccattttt cagtcccgta tctgcgccac gcatcccctg
     1501 aacggctgca cccgccaggc tcatcacctc accctgcaga tcggtgaacg cagaatgacg
     1561 ctggtcgatc tgcccggcat tggtgagaca ccgcagcatg atcaggaata ccggacactt
     1621 tatcgtcagc tgctaccgga actggatctg attatctgga tcctgcgggc agatgaacgc
     1681 gcgtatgccg ccgacattac catgcatcag tttttactga atgaaggcgc agatccctcg
     1741 cgttttctgt ttgttctcag ccatgccgat cgcgtgtttc ctgctgaaga atggaatgcc
     1801 acagaaaaat gcccgtcccg tcagcaggaa ctctcactgg cgacagtaac agcccgggtg
     1861 gccaccctgt tcccgtcatc atttccggta ctctccgttg cagcgcctgt cggctggaat
     1921 cttccggcac tggtgtcact gatgatccac gcactgccac cacaggcaac cagcgcggtt
     1981 tattcacata tccggggaga aaaccgctct gaacaggccc agaaacacgc acaacagact
     2041 tttggtgacg ccatcgggaa aagttttgac gacgccgttg cccggttcag ttttccggcc
     2101 tggatgttac agcttctgcg taaagcccgg gaccgcatta tccacctgct ggtcacactg
     2161 tgggatcgtc tgttctgaac gccccggagt cccccgtggc aaccgtatac ctttacacga
     2221 caacggatta cggtaaccac tgccgtatac cgtctgtcat tatcaataac acgctcttta
     2281 atttcagagg tcagtcatga ataccagaat tctttttccc ctgctgttca ctgtggcatc
     2341 cttctccgcc tccgccggca actgggctgt caaaaacggc tggtgtcaga ccatgacgga
     2401 agatggtcag gcgctggtaa tgctgaaaaa tggcacgatt ggtattaccg gcctgatgca
     2461 gggatgcccg aatggcgtac agacgctcct gggcaaccgt atcagtatta acggtaacct
     2521 gatccccaca tcacaaatgt gtaatcagca gacaggattc agggctgttg aggtggaagt
     2581 cggacaggcg ccggaaatgg tcaaaaaagc cgttcactcc atagcagagc gtgatgtgtc
     2641 cgttttacag gcatttggtg tacgaatgga attcacccgc ggtgatatgc tgaaggtctg
     2701 tccgaaattt gtcacatcac ttgccggttt ttccccgaaa cagacacccg ccattaataa
     2761 agattccgtc ctgcaggctg cccggcaggc atacgcccgg gaatatgacg aggaaacaac
     2821 agaaaccgct gattttggct cttacgaagt aaaaggcaat aaggttgagt ttgaagtgtt
     2881 caatcctgaa gaccgtgcgt acgacaaagt gaccgtcacg gttggtgctg acggtaatgc
     2941 caccggtgcc agcgttgaat ttatcggaaa atagccggta tgtcggactg tcaccctgtt
     3001 ttattgcccg aaggcccttt ctcacgcgaa caggcgatgg ctgtcacaac agcttaccgc
     3061 aatgtgctta ttgaagatga tcagggaacg catttccggc tggttatccg caatgctgaa
     3121 gggcagctac gctggcggtg ccggaactct gaagctgatg ccggaaaaca gcttaatgcg
     3181 tggctcg
//
LOCUS       DAOTRK010000269         3137 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.272, whole genome shotgun sequence.
ACCESSION   DAOTRK010000269 DAOTRK010000000
VERSION     DAOTRK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.272"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            766..1638
                     /locus_tag="QIE41_004668"
     CDS             766..1638
                     /locus_tag="QIE41_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDS3533443.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            1966..>3137
                     /gene="ag43"
                     /locus_tag="QIE41_004669"
     CDS             1966..>3137
                     /gene="ag43"
                     /locus_tag="QIE41_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDS3533444.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS"
BASE COUNT          818 a          808 c          811 g          700 t
ORIGIN      
        1 aaaatgcacc attttttctt caaatacttc tgtataatcg ccagccaatg atgagaatgg
       61 attaaaataa cacttcttcg acaaacacaa aaacagcctt cattttacat catcatccag
      121 tttcagttaa ccccggacga gcgacagtgt ttcagtacag gaattaaaag ccagcttttg
      181 ctcagtaaaa acacactcaa ctaataatat ctcagcagat attatcctga ccaggacacc
      241 tcacattact gaattctctt ccgacagtat cactgcacaa ataaaaaaga acgggataaa
      301 tattcagacc ggaagaatgg tgtcacaaaa gcatttaatc attacgcatg accaggaagg
      361 gaaacactta accacatggt atcaacagat atcattacgt gtgcactctt ataaaaaaca
      421 cacagaagaa gtaagataat tataaatgta aatttaatat ctcccactga catctccccc
      481 acaatacact gaaaaatact cacagcagta aacacagaca cacctgataa tcatgcagcc
      541 gataaatatt ttccccttta cggagaaatc agcctgctgt ttaacaataa cggcgtattt
      601 tcatggaagg agatcaccca gtaacggata agtcctgatc tccttcatgt taatacgcaa
      661 caatcccatc gattaaaaac aatttcagac agatattatc tccgtgaatc ccccgccacc
      721 tttccggtgc gcggggtttt gtcttttttc accgggaata catgtatgaa tccgtctgat
      781 gccattgagg caattgaaaa accgctctcc tccctgcctt actcgctttc ccgtcacatc
      841 ctggaacatc tgcgcaaact cacccgtcac gaacccgtga ttggcattat gggtaaaagc
      901 ggggccggta aatcctcact ctgtaatgca ctgtttcagg gggaggtcac cccggtcagt
      961 gatgttcacg ccggcacccg ggaagtacgg cgcttccgtc tgagtggcca cggtcacagc
     1021 atggttatca ctgacctgcc cggggtgggc gagagccggg acagggatgc agagtatgag
     1081 gccctgtacc gtgacattct gcctgaactt gacctggtac tgtggctgat taaagccgat
     1141 gaccgtgccc tgtctgtgga tgagtatttc tggcgacaca tcctgcaccg gggacatcag
     1201 cgggtactgt ttgtggtgac gcaggccgac aaaacggagc cctgccatga atgggatatg
     1261 gccggtattc agccttctcc tgcacaggca cagaatattc gcgaaaaaac ggaggcggta
     1321 ttccgtctgt tccggcccgt acatccggtt gtggccgtat cggcccgcac cggctgggaa
     1381 ctggatacgc tggtcagtgc gctcatgaca gcgcttcccg accatgccgc cagtcccctg
     1441 atgacccgac tgcaggacga gctgcgcacg gaatctgtcc gctctcaggc ccgtgaacag
     1501 tttaccggtg cggtggaccg gatatttgac acagcggaga gcgtctgtgt tgcctctgtt
     1561 gtccgtacgg ccctgcgcgc tgttcgtgac acggtggtct ctgttgcccg tgcggtatgg
     1621 aactggattt tcttctgaac ctgtcgtgac tgatgccctc cctgactctg agtctgctca
     1681 caaaagcact gttttcgtta ctgtctctct tgtccgtgca atagctcaat aatagaataa
     1741 aacgatcgat atctatttta tcgatcgttt atatcgatcg ataagctaat aataaccttt
     1801 gtcagtaaca tgcacagata cgtacagaaa gacattcagg gaacaacaga accacaattc
     1861 agaaactccc acagccggac ctccggcact gtaacccttt acctgccggt atccacgttt
     1921 gtgggtaccg gcttttttat tcaccctcaa tctaaggaaa agctgatgaa acgacatctg
     1981 aatacctgct acaggctggt atggaatcac attacgggcg ctttcgtggt tgcctccgaa
     2041 ctggcccgcg cacggggtaa acgtggcggt gtggcggttg cactgtctct tgccgcagtc
     2101 acgtcactcc cggtgctggc tgctgacatc gttgtgcacc cgggagaaac cgtgaacggc
     2161 ggaacactgg caaatcatga caaccagatt gtcttcggta cgaccaacgg aatgaccatc
     2221 agtaccgggc tggagtatgg gccggataac gaggccaata ccggcgggca atgggtacag
     2281 gatggcggaa cagccaacaa aacgactgtc accagtggtg gtcttcagag agtgaacccc
     2341 ggtggaagtg tctcagacac ggttatcagt gccggaggcg gacagagcct tcagggacgg
     2401 gcagtgaaca ccacgctgaa tggtggcgaa cagtggatgc atgagggggc gatagccaca
     2461 ggaaccgtca ttaatgataa gggctggcag gtcgtcaagc ccggtacagt ggcaacggat
     2521 accgttgtta ataccggggc ggaaggggga ccggatgcag aaaacggtga taccgggcag
     2581 tttgttcgcg gggatgccgt acgcacaacc atcaataaaa acggtcgcca gattgtgaga
     2641 gctgaaggaa cggcaaatac cactgtggtt tatgccggcg gcgaccagac tgtacatggt
     2701 cacgcactgg ataccacgct gaatggggga taccagtatg tgcacaacgg cggtacagcg
     2761 tctgacactg ttgtgaacag tgacggctgg cagattgtca aaaacggggg tgtggccggg
     2821 aataccaccg ttaatcagaa gggcagactg caggtggacg ccggtggtac agccacgaat
     2881 gtcaccctga agcagggcgg cgcactggtt accagtacgg ctgcaaccgt taccggcata
     2941 aaccgcctgg gagcattctc tgttgtggag ggtaaagctg ataatgtcgt actggaaaat
     3001 ggcggacgcc tggatgtgct gaccggacac acagccacta atacccgcgt ggatgatggc
     3061 ggaacgctgg atgtccgcaa cggtggcacc gccaccaccg tatccatggg aaatggcggt
     3121 gtactgctgg ccgattc
//
LOCUS       DAOTRK010000270         2913 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.273, whole genome shotgun sequence.
ACCESSION   DAOTRK010000270 DAOTRK010000000
VERSION     DAOTRK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.273"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..907
                     /locus_tag="QIE41_004670"
     CDS             <1..907
                     /locus_tag="QIE41_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDS3533445.1"
                     /translation="FSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSKTLNELE
                     QLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDVVRVGAL
                     PTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSDPELMTG
                     LNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDALVQSQGC
                     KIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGAGEPIGI
                     LTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(946..2586)
                     /gene="trg"
                     /locus_tag="QIE41_004671"
     CDS             complement(946..2586)
                     /gene="trg"
                     /locus_tag="QIE41_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDS3533446.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSARANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FIVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVAMAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVLAEPRGAGEQVSFATV"
BASE COUNT          701 a          764 c          689 g          759 t
ORIGIN      
        1 gtttagtcag cgcatccgtt tgcgccacct tcatacattc gtagctgtcg cacaacaagg
       61 aactttgggg cgcgcggctg aaacccttaa tttgagtcaa cctgcgctct ctaaaacatt
      121 gaatgaactg gagcaactga cgggcgctcg cttgtttgag cgtggtcgtc agggggcgca
      181 acttacctta cccggcgaac aatttttaac gcatgcagtc agagttcttg acgccatcaa
      241 cactgccgga cagtcgcttc atcgtaaaga aggtcttaat aatgatgtcg tcagggttgg
      301 tgcactacct actgcggcac tggggatatt accttcggtt ataggtcagt ttcatcagca
      361 acaaaaagaa acgaccttgc aagttgcgac aatgagtaac cctatgattc tggcgggttt
      421 gaaaaccggg gaaatcgata tcggcattgg tcggatgtca gatcctgaac tgatgaccgg
      481 gcttaattac gaactgctgt ttcttgaatc gctgaagctg gttgtccgcc ccaatcatcc
      541 gctacttcag gagaacgtaa cgctaagccg ggtgctggaa tggccggtcg ttgtatcacc
      601 agaaggcact gcgccacgcc agcattcaga tgcattagtg cagagccagg gatgtaaaat
      661 tccttcgggt tgtatcgaaa cgctgtctgc ttcgctatct cgtcaactta cggttgaata
      721 cgactacgtg tggtttgtcc cttctggcgc ggtaaaagac gacctgcgtc atgccacgct
      781 ggtggccctg cctgttccgg gacatggtgc aggcgaaccg attggaatac tgacccgcgt
      841 agatgcaacg ttctcttctg gttgccagtt gatgattaac gctattcgaa aatcaatgcc
      901 gttctgaaag gtgaagggat ctgtcgatcc cttccttgaa cattttcaca ccgtagcgaa
      961 actaacttgt tcacccgctc cgcgaggttc tgccaacaca gaatgtttgt tcagacggaa
     1021 tacgtccacc gcctcagtta atcgtgccgc ctgttcttca agggacaccg ccgccgctga
     1081 ggcctcttct accagagaag cattctgttg cgtcacctta tccatttcag aaatcgcctg
     1141 gctaacctga gttatgcctc tgctttgttc atccgaggca gcggcgattt cctgcatgat
     1201 atgtgtgaca ctcgcgacgg catcaacaat agtgctcatg gttttccctg ccattgccac
     1261 ctcatccgac cccaggtcaa ttaacctgac tgattcactg atcaagcctt caatctcttt
     1321 cgccgcctga gcgctgcggc ttgcgagtgt ccgtacttcg ctggcgacaa cggcaaatcc
     1381 acgcccttgc tcacccgctc gcgcggcttc aacggcagca ttcagtgcca gaatattcgt
     1441 ctggaaagca atactgttga tgacggcggt gatctcagaa attttcttcg aactcgtgga
     1501 gatagcgccc atcgttttta ctacaccgga aaccgtctgc ccgccatcgc tggctttaat
     1561 agaagcctct tgcgccagtt tgctggcatg atgcgcgtta tccgcattct gtttcaccgt
     1621 cgcagtaagt tgctccatgc tggcggcagt ttgttcgata gccgccgctt gttcttcggt
     1681 gcgagatgac aggtccgcat tgccagctga aatttcgctg gtgccacgat aaatctcttc
     1741 cgcaccctgt cgaacagtcc ctactgtcat ccccagtgaa tgctgcattt gctgtaaatg
     1801 acgacttaag cgaccgattt cattacgacc cgccggttca tcattcatcg tcagatcgcc
     1861 actggcgatt ttttcaatcc gttgtgcggc atgttgcagt ggacgaatga cgatccgacg
     1921 tagcactata aatgttatca gcgtcatgac cagggcaagc acaaacgcgc caatcatgaa
     1981 catcccaccc aggcgggtgc gctgatgggc cagttccgcc agttggttgg ctctggcgct
     2041 acgtatctta acggctttgc tcaaaatatc ggtataagca ttatccagcg gtcggatctg
     2101 ctcactttca tgattgataa tcgcttcaaa catgccattt ttggcatatt tcaacatcgg
     2161 ttgcataccc gtgatataag cctggaagcg ttgatttaat tcagtgtcga gggcttcatc
     2221 agcaggtgtt ttcaccgacc gattctgata agcacgataa ccttgctgcg actgtttaat
     2281 ctccgattcg gcttgcgcaa tatttcgctt cattgcttcc atttcagcaa tacgactcgc
     2341 agcaccagcc tgaatcatat tgatccgggc tgaacgcaaa aaatctgaac tgttcgctaa
     2401 cccggtccga atctcaatct ccgcagtaac atcacgctga tcgcgatcgg cctgccaaag
     2461 gaaatagccg gccagggctg aacttaatgc gaatagaact aagataccgc caagaatgca
     2521 ggcaaataac ggaaccagcc tgatgtgatg caaaaaacct aatcgctgtg agggagttgt
     2581 attcattacc gttgtctctc ttccaggttt actcctgata gaaagtcatc ggcacttgac
     2641 ggtaatttct tacgaccaaa aacctctttg cgtcacattt ttacaacaat tatggcagag
     2701 aagaataatg ccattggaat gaagagagta acgaatgatt aaactggcta aaccgaatga
     2761 cgaaaaattc tggaaaacag cacgcaattt cgtgaagtga ttattttcgt ccctaaggaa
     2821 acctcatagg atgcctgcgg gtgcctgaga ctttctgttt tcaggctaac cgggaatcag
     2881 aaagacgaag gccccgaccg atatttctat caa
//
LOCUS       DAOTRK010000271         2900 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.274, whole genome shotgun sequence.
ACCESSION   DAOTRK010000271 DAOTRK010000000
VERSION     DAOTRK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.274"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..377)
                     /gene="matP"
                     /locus_tag="QIE41_004672"
     CDS             complement(<1..377)
                     /gene="matP"
                     /locus_tag="QIE41_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDS3533447.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDA"
     gene            563..2323
                     /locus_tag="QIE41_004673"
     CDS             563..2323
                     /locus_tag="QIE41_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDS3533448.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLPSELLKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            2392..>2900
                     /gene="fabA"
                     /locus_tag="QIE41_004674"
     CDS             2392..>2900
                     /gene="fabA"
                     /locus_tag="QIE41_004674"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDS3533449.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTS"
BASE COUNT          687 a          718 c          764 g          731 t
ORIGIN      
        1 gcatcttcaa tcagctgaac aatcgtttca gaaagcgttt taccgcgacg ctgcgcaaga
       61 ccagccagac gttgccagac aataaattcc agatcgatcg attttttgcg cgtatgctgg
      121 tgttccgcat taaaatggcg cttccgtctg gcccgaatgg tttgcttcat gcgattgacc
      181 agttccggat tcatatgctt gtcaatccag ccatttacca gcacgggttc attttccagc
      241 gagagcaaca catcgacggc ttcctgggcg gcactggctt ctatgtaacg ggtgattaac
      301 tccccttcgc gatgcttctt caccagatac ttccatttcc aaccgctttc aagattttca
      361 agttgttgat atttcatagc gatctcaatg ttaccgtgta actctttaca gaatatcagc
      421 tttttgggcc agtgaagaaa agaatctcca tcctgtgagc tttaacgccc atgccaggag
      481 tattgtcggt gctttctacg tgtgctgcag tgccggttac ggtataatcg cggctttgac
      541 aacagactaa aaaacatcaa ctttgaccat tacgaaactt gcatggcgtg acctggttcc
      601 tgataccgat agctatcagg aaatatttgc tcagccacat ttgattgacg aaaacgatcc
      661 tttattcagt gatactcaac cgcgactgca atttgcgctg gagcagttgc tgcatacgcg
      721 agcatcctcc tcttttatgc tggcgaaggc cccggaagag tctgagtatc tgaatcttat
      781 tgccgatgcc gcgcgtgcgc tacaaagcga tgcaggccaa ctggtgggcg gtcactatga
      841 ggtttccggg cacaccatcc gcttacgtaa cgcagtgagt gcagatgata attttgcgac
      901 tttaacgcaa gttgtcgctg ccgactgggt agaagcggaa caactctttg gctgcctgcg
      961 ccagtttaat ggcgacatta ccctgcagcc aggtctggtg catcaggcaa atggcggtat
     1021 tctcatcatc tctttgcgta cactgctggc gcaacctctg ctgtggatgc ggctgaaaaa
     1081 tatcgttaac cgcgagcgtt ttgactgggt tgcgtttgat gagtcgcgcc ctctccccgt
     1141 ctctgtgcct tcgatgccat tgaagctgaa agtcattctg gtaggcgaac gcgaatcatt
     1201 ggctgatttc caggagatgg aaccagagct ttcagagcag gctatttata gcgaatttga
     1261 agatactctg cagattgtcg atgcggagtc agtaagccag tggtgtcgct gggtaacatt
     1321 gaccgccaga cataatcact tacctgcacc gggagcggat gcctggccgg tgcttatccg
     1381 cgaagcagca cgctacaccg gtgaacaaga aacacttccg cttagcccgc agtggatcct
     1441 ccgccagtgt aaagaggtcg cctccctgtg cgatggcgac accttctccg gcgagcagct
     1501 aaacttaatg ctacagcagc gtgaatggcg cgaaggtttc ctcgctgaac gcatgcagga
     1561 tgagatcctt caggagcaaa tcctgattga aaccgaaggc gaacgcatcg ggcaaattaa
     1621 cgccctttcg gtcattgaat ttccgggtca tccacgcgct tttggcgaac cttctcgcat
     1681 tagctgcgtt gtgcatattg gcgatggtga gttcaccgac atcgaacgca aagcggagct
     1741 tggcggcaat atccatgcga aagggatgat gatcatgcag gcgttcctga tgtcggaact
     1801 acagcttgag caacagatcc ccttctcagc atcgctgaca tttgagcagt catacagtga
     1861 agtggatggc gatagtgcct cgatggctga actctgcgcc ctgataagcg ccctcgccga
     1921 tgtgccggtg aatcagagta tcgctatcac aggttcagtc gatcagttcg gtcgtgccca
     1981 gccggtcggt ggtttaaatg agaaaatcga aggcttcttt gctatttgcc agcaacgtga
     2041 gttaaccggg aaacaaggtg tcattatccc cactgctaac gttcgccatt taagtcttcc
     2101 cagtgaactg ttgaaagcgg tagaggaagg caaattcacc atctgggcag tagacgatgt
     2161 gactgacgca ctgccgttat tattaaatct ggtgtgggat ggcgaaggcc aaacgacgct
     2221 gatgcaaacc atccaggaac gtatcgcgca agcatcgcaa caggaaggac gtcaccgttt
     2281 tccatggcca ttacgttggc tgaactggtt tattccgaac tgatcggact tgttcagcgt
     2341 acacgtgtta gctatcctgc gtgcttcaat aaaataaggc ttacagagaa catggtagat
     2401 aaacgcgaat cctatacaaa agaagacctt cttgcctctg gtcgcggtga actgtttggc
     2461 gctaaaggcc cgcaattgcc agcaccgaac atgctgatga tggaccgtgt ggtcaaaatg
     2521 accgaaacgg gtggtaactt cgacaaaggg tatgttgaag cagaactgga tatcaatccg
     2581 gatctgtggt tcttcggatg ccactttatt ggcgatccgg ttatgccggg atgcctgggc
     2641 ctggacgcaa tgtggcagct ggtagggttc tacctcggct ggctgggcgg cgaaggtaaa
     2701 ggccgcgcgc tgggcgttgg cgaagtgaaa ttcactggtc aggtactgcc gacagcgaaa
     2761 aaagtgacct accgtattca ctttaaacgc attgttaacc gtcgtctgat tatgggcctg
     2821 gcggatggcg aagtgctggt tgatggtcgt ttgatctata ccgccagcga cctgaaagtc
     2881 ggtctgttcc aggatacgtc
//
LOCUS       DAOTRK010000272         2898 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.275, whole genome shotgun sequence.
ACCESSION   DAOTRK010000272 DAOTRK010000000
VERSION     DAOTRK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.275"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            136..1452
                     /locus_tag="QIE41_004675"
     CDS             136..1452
                     /locus_tag="QIE41_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDS3533450.1"
                     /translation="MDALFFIQLFIVLACIGIGGKYGGMGLGAGGGLGVAILVLGFGL
                     KPSSPSITAMLIIICVISAVSILQAVGGLDYLVRVAERILRKKPQAITFVAPILTSVF
                     TLFCGTTYVAFSLYPVIAEVAAEAKVRPERALSATVIAASVAVAASPMSAATAGMLAI
                     LHEYAGITLGQILSIALPSFFMAAIVTSFSVYKRGKELEDDPEFQRRVAAGEYEFMHT
                     EQKKEYVAAPGARKGVAIFAIGVVLVLILGSFTELLPSWDGKRLSTPMVIQMIMLTAA
                     LLIMIVGKVPSNKFNSGSVFRAGLMGVVAILGVSWMTATFFDAYQAELINVFGNLVND
                     APLLFGFIVFLFSLVIMSPAATVAAIMPLGVTLGIPAPYLIAIFACTCGDFIIPGANQ
                     IGCVAFDRTGTTRIGRFVINHSYIRPGFVMVISQVVFAYLIAQVIL"
     gene            complement(1568..2884)
                     /locus_tag="QIE41_004676"
     CDS             complement(1568..2884)
                     /locus_tag="QIE41_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDS3533451.1"
                     /translation="MMLFVIQFAIVLTCIGIGGRFGGIGLGAAGGLGLAILTFGFGVP
                     PDSPPITVISIILAVITCIAILQAAGGLDLFVTIAEKILQKRPGAITFLGPAVAYLFT
                     AICGTGYVAFSIYPVIAEIAADARVRPERAMSMSVIAANFGLIASPVSAVVTGTIAVF
                     SGLHVSALDILLITVPGTILGCLVGCLFVYKRGHDLETDPEFQRRVAEGEFESVKTGE
                     RTISIISKTAKKALMIFISGIILVVVLGSVPELRPVWNTSAGVERMSIPTALQIIMLT
                     TACIIMMVCRISPSKLDSGSVFKAGLVGVVAIFGLSWMMSSFFEAWQDLFNNTFNDFH
                     NPVIFGVIVFVLSAVIYSPAATAVALFPAGVLMGYSTETLIALLPVTCGSFIIPGGAQ
                     IACVAFDRTGTTRVGKYVVNHSYMLPGLITVLASTIFCFLFSTILV"
BASE COUNT          842 a          612 c          629 g          815 t
ORIGIN      
        1 tgtcttatgg tggaaccgga cctgatgctg tcaaaaaaca aatagagatt gcaaaatcac
       61 ttttaaaata gttaagaatt ataatgttta agtttccccg gatattatat ccgggaatat
      121 ctttgaggtt gaaaaatgga cgctctattt tttatacaac tatttattgt tctggcctgt
      181 attggaatcg gcggaaaata cggcggaatg gggcttggtg ctggaggtgg gttaggggtt
      241 gcaatattgg ttttgggctt tggactgaaa ccctcctctc cctccattac tgctatgtta
      301 attattattt gtgttattag tgctgtcagt atattacaag ctgttggagg attagattat
      361 ttagtcaggg tcgcagaacg gatactccgt aagaaaccac aggcgataac ctttgtagcg
      421 ccaatattaa cttcagtatt tacactattt tgtgggacga cttatgtcgc tttctcctta
      481 taccctgtta ttgctgaagt cgccgctgaa gcaaaagtac gtccagaacg tgcattatct
      541 gcaacggtca ttgcagccag tgtggcagtg gctgccagcc ctatgagtgc agcaactgcc
      601 gggatgctgg ctatacttca tgagtatgca gggatcacgt tagggcaaat attatctatt
      661 gcccttcctt cgttttttat ggctgcaatt gttaccagct tttcagtcta caagcgtggt
      721 aaagaactgg aagatgatcc tgaatttcaa aggcgtgttg cagcaggtga gtatgagttc
      781 atgcacaccg aacagaaaaa agaatatgtc gctgcaccag gtgcaagaaa aggggttgct
      841 atttttgcca taggagtcgt gctggtcctt attctcggat cttttactga gcttcttccg
      901 tcgtgggatg gaaaaagatt atcaacccca atggtcatcc agatgattat gttgactgca
      961 gctttattga tcatgattgt ggggaaagtt ccaagcaata aatttaacag tggctctgtg
     1021 ttccgtgctg gtttgatggg ggttgtggca atacttggtg tttcgtggat gactgcaaca
     1081 ttttttgatg cttatcaggc agagctgatc aatgtttttg gcaatcttgt aaatgatgcg
     1141 cctttgctgt ttggctttat cgtattcttg ttttctctgg tgatcatgag cccggctgcg
     1201 acagtagctg cgattatgcc attaggagtc accctgggta ttcctgcacc ttatcttatt
     1261 gcaatttttg cctgtacttg tggtgatttt attatacccg gagcgaatca gattggttgt
     1321 gtggcgtttg acagaactgg tactaccaga attgggcgct ttgttatcaa ccatagctat
     1381 atacgtcctg ggttcgtcat ggtcatttcc caggttgttt ttgcctactt aattgcgcag
     1441 gttattctgt aacattaagg aataaatgaa gaggataaca ctgtggcatg acgaattagc
     1501 ccccttcagc gtttttgagg aagggggatt aagaaaatat tacacagctg taagaatact
     1561 tctctgatta cactaatata gtggaaaaaa ggaagcagaa gatagttgaa gccagaacgg
     1621 taatcagacc gggtaacata taactgtgat ttactacata cttgccaact ctggttgttc
     1681 ctgttctgtc aaacgccacg caagcaattt gtgcaccacc aggaataatg aatgatccgc
     1741 acgttaccgg aagcaaagct atgagcgtct cagtcgaata tcccattaat acgccagcag
     1801 gaaataatgc cacagcagtt gctgcgggac tgtaaatcac agcagaaaga acaaacacaa
     1861 tcacaccgaa tataactggg ttatgaaaat cattgaaagt gttattaaat aaatcttgcc
     1921 atgcttcaaa gaaagaactc atcatccatg aaagaccaaa tatggcaaca actccaacca
     1981 gaccggcctt aaaaacggat cctgagtcaa gtttcgatgg agaaatccga cataccatca
     2041 taatgataca tgctgtagtc aacatgatga tttgcaatgc tgttggaata ctcatccgtt
     2101 ctacaccggc actggtattc cataccggac gtaattctgg aacagagcct aaaacaacaa
     2161 ccaaaatgat cccagaaata aaaatcatca gggctttctt cgctgtttta gatataatac
     2221 tgattgtacg ttccccagtt tttactgact caaattcacc ttctgcaact ctgcgttgaa
     2281 actctgggtc cgtttcaaga tcatggcccc gtttataaac aaacaaacaa ccaacaagac
     2341 accccaaaat agttcccggt acagtaatca ataaaatatc taaagcagaa acatgtaatc
     2401 cggaaaagac cgcaatcgtt cctgtaacga ccgcacttac tgggctcgct ataagaccaa
     2461 agttggcggc aatgactgac attgacatgg cgcgttctgg cctcactctt gcatcagctg
     2521 cgatttcagc aataacagga taaatggaaa aagcgacata accagttcca catattgccg
     2581 taaaaagata agcaactgct ggcccaagaa acgtgattgc accaggcctc ttttgcagta
     2641 ttttttcagc tattgtcacg aaaagatcaa gacctcccgc tgcctgcagg atagcaatac
     2701 atgtgataac agcaagaata atggaaatga cagtgatagg cggggaatca ggcgggactc
     2761 caaaaccaaa agtcagaatt gcgagcccta atccccccgc cgcccctaag ccgattccac
     2821 caaagcgccc acctatccca atgcatgtaa gtactattgc aaactgaatt acgaataaca
     2881 tcataaccaa tccctttc
//
LOCUS       DAOTRK010000273         2876 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.276, whole genome shotgun sequence.
ACCESSION   DAOTRK010000273 DAOTRK010000000
VERSION     DAOTRK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.276"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            162..1826
                     /gene="asnB"
                     /locus_tag="QIE41_004677"
     CDS             162..1826
                     /gene="asnB"
                     /locus_tag="QIE41_004677"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDS3533452.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVTDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2207..2283
                     /locus_tag="QIE41_004678"
     tRNA            2207..2283
                     /locus_tag="QIE41_004678"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2241..2243,aa:Met,seq:cat)
     gene            2292..2376
                     /locus_tag="QIE41_004679"
     tRNA            2292..2376
                     /locus_tag="QIE41_004679"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2326..2328,aa:Leu,seq:tag)
     gene            2400..2474
                     /locus_tag="QIE41_004680"
     tRNA            2400..2474
                     /locus_tag="QIE41_004680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2432..2434,aa:Gln,seq:ttg)
     gene            2509..2583
                     /locus_tag="QIE41_004681"
     tRNA            2509..2583
                     /locus_tag="QIE41_004681"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2541..2543,aa:Gln,seq:ttg)
     gene            2599..2675
                     /locus_tag="QIE41_004682"
     tRNA            2599..2675
                     /locus_tag="QIE41_004682"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2633..2635,aa:Met,seq:cat)
     gene            2724..2798
                     /locus_tag="QIE41_004683"
     tRNA            2724..2798
                     /locus_tag="QIE41_004683"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2756..2758,aa:Gln,seq:ctg)
BASE COUNT          733 a          716 c          730 g          697 t
ORIGIN      
        1 taaatacgcc accacaccct atcctttttc acaaatcagc aatcgccatt acgtttttta
       61 tttttccact gccaaatagc ttgcgcacag tgctgctttg cggcattttt ttaaacaagc
      121 aaacacaaca agcaacaaat accaggttaa cggagaaggt tatgtgttca atttttggcg
      181 tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc
      241 tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac gccattctcg
      301 cccacgaacg tctttccatt gttgacgtta acgcgggggc gcaacctctc tataaccaac
      361 aaaaaactca tgtgctggcg gtaaacggtg aaatctacaa ccaccaggca ttgcgcgccg
      421 aatatggcga tcgttaccag ttccagaccg ggtctgactg tgaagtgatc ctcgcgctgt
      481 atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc tttgccctgt
      541 acgacagcga aaaagatgcc tacctgattg gtcgcgacca tctggggatc atcccactgt
      601 atatgggcta tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa gccctggtgc
      661 cagtttgccg cacgattaaa gagttcccgg cggggagcta tttgtggagt caggacggcg
      721 aaatccgttc ttattatcac cgcgactggt tcgactacga tgcagtgaaa gataacgtga
      781 ccgacaaaaa cgagctgcgt caggcactgg aagattccgt taaaagccat ctgatgtctg
      841 atgtgcctta cggtgtgctg ctttctggtg gtctggattc ctcaattatt tccgcgatca
      901 ccaagaaata cgccgcccgt cgcgtggaag atcaggaacg ttctgaagcc tggtggccgc
      961 agttacactc ctttgctgta ggtctgccgg gttcaccgga tctgaaagca gcccaggaag
     1021 tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag gaaggtctgg
     1081 atgccatccg cgacgtgatt taccacatcg aaacctatga tgtgaccact attcgcgctt
     1141 caacaccgat gtatttaatg tcgcgtaaga tcaaggcgat gggcattaaa atggtgctgt
     1201 ccggtgaagg ttctgatgaa gtgtttggcg gttatcttta cttccacaaa gcgccgaacg
     1261 ctaaagaact gcatgaagag acggtgcgta aactgctggc cctgcatatg tatgactgcg
     1321 cgcgtgccaa caaagcgatg tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca
     1381 aaaaattcct tgatgtggcg atgcgcatta acccgcagga taaaatgtgc ggtaacggca
     1441 aaatggaaaa acacatcctg cgtgaatgtt ttgagtcgta cttgcccgca agtgtggcct
     1501 ggcggcagaa agagcagttc tccgatggcg tcggttacag ttggatcgac accctgaaag
     1561 aagtggcggc acagcaggtt accgatcaac aactggaaac cgcccgcttc cgcttcccat
     1621 acaacacgcc aacctctaaa gaagcgtact tgtatcggga gatctttgaa gaactgttcc
     1681 cgcttccgag cgccgctgag tgcgtgccgg gcggtccttc cgtcgcgtgt tcttccgcta
     1741 aagcgatcga atgggatgaa gcgttcaaaa aaatggacga tccatctggt cgtgcggttg
     1801 gtgttcacca gtcggcgtat aagtaagaaa tgcgaaacag ccccggtaaa atccggggct
     1861 gtttcagtta ttgaggtgct tgttgattct gatatgcagt aaggaaatgt tccgggtttg
     1921 ccacatcact gtttgacaat tcatcactgt aggccagtac ttcatagatt aatttcccac
     1981 cactattgtt gaattccgaa tttcatcctg tatgacaaaa gaatggctgt agggtgggga
     2041 aaaaacacaa agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca
     2101 aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg
     2161 cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aaagatggct acgtagctca
     2221 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
     2281 ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg
     2341 caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg ttgtacggat
     2401 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc
     2461 aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc
     2521 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
     2581 ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga
     2641 tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatatgttc
     2701 ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat
     2761 tccggcattc cgaggttcga atcctcgtac cccagccaat ttattcaaga cgcttacctt
     2821 gtaagtgcac ccagttgggg tatcgccaag cggtaaggca ccggattctg attccg
//
LOCUS       DAOTRK010000274         2834 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.277, whole genome shotgun sequence.
ACCESSION   DAOTRK010000274 DAOTRK010000000
VERSION     DAOTRK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.277"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..336
                     /gene="dgoR"
                     /locus_tag="QIE41_004684"
     CDS             <1..336
                     /gene="dgoR"
                     /locus_tag="QIE41_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDS3533453.1"
                     /translation="SSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNPVL
                     QQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMIAS
                     STRRLKEIT"
     gene            333..1211
                     /gene="dgoK"
                     /locus_tag="QIE41_004685"
     CDS             333..1211
                     /gene="dgoK"
                     /locus_tag="QIE41_004685"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDS3533454.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNEKSPA
                     AVLAEVTADWREENTPVVMAGMVGSNVGWKVAPYLSVPVRFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAVVAGD
                     KAFQAGIRSIAHAVAN"
     gene            1195..1812
                     /gene="dgoA"
                     /locus_tag="QIE41_004686"
     CDS             1195..1812
                     /gene="dgoA"
                     /locus_tag="QIE41_004686"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDS3533455.1"
                     /translation="MQWQTKLPLIAILRGITPDEALVHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDVLARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            1809..>2834
                     /gene="dgoD"
                     /locus_tag="QIE41_004687"
     CDS             1809..>2834
                     /gene="dgoD"
                     /locus_tag="QIE41_004687"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDS3533456.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNSPV
                     WQLMGGLVRDKIKAYSWVGGDRPANVIDGIKILREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKP"
BASE COUNT          648 a          728 c          827 g          631 t
ORIGIN      
        1 tccagcgatc tggcgcagat tgaatcggcg ctgaacgaga tgattgccaa caatcaggac
       61 cgcgaagcgt ttaacgaagc ggatattcgc taccacgagg cggtgctgca gtcggtacat
      121 aatccggtgt tacagcaact tagcattgcg atcagttcgc tgcagcgggc ggtatttgaa
      181 cgaacctgga tgggtgatga ggccaacatg ccgcaaacgc tccaggaaca taaggcgctg
      241 ttcgatgcga tacggcatca ggacggcgat gcggcagagc aggcggcgct taccatgatc
      301 gccagctcga cacgaaggtt aaaggaaatc acatgacagc tcgctacatc gcaattgact
      361 ggggatcgac caatctgcgc gcctggcttt atcagggcga ccactgcctg gagagcaggc
      421 aatcagaagc aggcgtcacg cgcctgaacg aaaaatctcc ggctgcggtg ttagcagaag
      481 tgacggccga ctggcgtgaa gaaaatacgc cagtggtgat ggcaggaatg gtcggcagca
      541 atgtcggctg gaaagttgcg ccgtatttat ctgttcctgt ccgtttttcg tctattggcg
      601 aacaattaac gtcggttggc gacaatatct ggattattcc cggattatgc gtttctcatg
      661 acgataacca caatgtgatg cgcggcgaag aaacacaatt gatcggcgcg cgtgctctgg
      721 ctccttcctc tctttatgtt atgcccggaa cccattgcaa atgggtgcag gccgatagcc
      781 agcaaatcaa cgattttcgc accgtaatga ccggtgaatt acatcattta ctgttaaatc
      841 actcattgat tggcgcaggt ttgccgccgc aggaaaactc tgccgatgcc ttcgcggctg
      901 gccttgagcg tggccttaat gcgcccgcca tattgccgca gctttttgaa gttcgcgcct
      961 cgcatgtgct gggaacgctt ccccgcgaac aggtcagcga atttctctct ggtttgttga
     1021 ttggcgcaga ggttgccagt atgcgcgact atgtgaccca tcaacacgcc atcacccttg
     1081 tcgccggaac atcgctgacc gcgcgctacc agcaagcctt tcaggcgatg ggttgcgacg
     1141 tggcggtggt ggcgggcgac aaggcatttc aggctggtat aaggagcatc gctcatgcag
     1201 tggcaaacta aactcccgct gatcgccatt ttgcgcggca ttacgcccga cgaggcgctg
     1261 gtgcatgttg gcgcggtgat tgacgccggg ttcgacgcag ttgaaatccc gctgaattcc
     1321 ccacaatggg agcagagcat tcccgccatc gttgatgcgt atggcgacaa ggcgttgatt
     1381 ggcgcaggta cggtactgaa acctgaacag gtcgatgtgc tcgccaggat gggctgccag
     1441 ctcatcgtta cgcccaatat ccatagtgaa gtgatccgcc gtgcggtggg ctacggcatg
     1501 accgtttgcc ccggctgcgc gacggcgacc gaagccttta ccgcgctcga cgcgggcgct
     1561 caggcgctga aaatatttcc gtcatcggct tttggtccgc aatacatcaa agcgttaaaa
     1621 gcggtattac caccggacat cgcagtcttt gccgttggcg gcgtgacgcc agaaaacctg
     1681 gcgcagtgga tagacgcagg ttgtgcaggg gcaggcttag gcagcgatct ctatcgcgcc
     1741 gggcagtccg tagaacgcac cgcgcagcag gcagcagcat ttgttaaggc gtatcgagag
     1801 gcagtgcaat gaaaatcacc aaaattacca cgtatcgttt acctccccgc tggatgttcc
     1861 tgaaaattga aaccgatgaa ggcgtggtcg gctggggcga gcccgtgatc gaaggccgcg
     1921 cccgtacggt ggaagcggca gttcacgagc tgggtgacta tttgattggt caggatccat
     1981 cgcgcatcaa tgacttatgg caagtgatgt atcgcgccgg attttatcgt ggaggtccaa
     2041 tcctgatgag cgccattgcc gggatcgacc aggcgttatg ggatatcaaa ggcaaagtgc
     2101 tgaattcgcc ggtctggcaa ctgatgggcg gcctggtgcg tgacaaaatt aaagcctaca
     2161 gttgggtcgg cggcgatcgt ccggcgaatg ttatcgacgg aataaaaatc ctgcgcgaaa
     2221 tcggttttga taccttcaaa ctgaatggtt gtgaagaact ggggctaatt gataactccc
     2281 gcgcggtaga tgcggcggtc aacaccgtgg cacaaattcg tgaagctttt ggcaatcaga
     2341 ttgagtttgg tcttgatttc cacggtcgcg tcagcgcgcc gatggcgaaa gtactgatta
     2401 aagaactgga accgtatcgc ccgctgttta ttgaagagcc ggtgctggca gaacaggccg
     2461 aatactaccc gaaactggcg gcacaaacgc atattccact ggcggcgggt gagcgcatgt
     2521 tctcacgttt cgattttaaa cgtgtgctgg aggcgggtgg tatttcgatt ctgcaaccgg
     2581 atctctccca cgcaggcggt attaccgaat gctacaaaat tgccggaatg gcagaagcct
     2641 atgacgtgac ccttgcgccg cactgtccgc tcggaccgat tgcactggca gcttgcctgc
     2701 atatcgactt tgtttcctat aacgccgtac ttcaggaaca aagtatgggc attcattaca
     2761 acaaaggcgc ggagttactc gactttgtga aaaacaaaga ggacttcagc atggtcggcg
     2821 gcttctttaa accg
//
LOCUS       DAOTRK010000275         2834 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.278, whole genome shotgun sequence.
ACCESSION   DAOTRK010000275 DAOTRK010000000
VERSION     DAOTRK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.278"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..336
                     /gene="dgoR"
                     /locus_tag="QIE41_004688"
     CDS             <1..336
                     /gene="dgoR"
                     /locus_tag="QIE41_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDS3533457.1"
                     /translation="SSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNPVL
                     QQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMIAS
                     STRRLKEIT"
     gene            333..1211
                     /gene="dgoK"
                     /locus_tag="QIE41_004689"
     CDS             333..1211
                     /gene="dgoK"
                     /locus_tag="QIE41_004689"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDS3533458.1"
                     /translation="MTARYIAIDWGSTNLRAWLYHGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQIFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            1195..1812
                     /gene="dgoA"
                     /locus_tag="QIE41_004690"
     CDS             1195..1812
                     /gene="dgoA"
                     /locus_tag="QIE41_004690"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDS3533459.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDVLARMGCQLIVTPNIHSEVIRRVVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSIERTAQQAAAFVKAYREAVQ"
     gene            1809..>2834
                     /gene="dgoD"
                     /locus_tag="QIE41_004691"
     CDS             1809..>2834
                     /gene="dgoD"
                     /locus_tag="QIE41_004691"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDS3533460.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNTPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKP"
BASE COUNT          649 a          733 c          823 g          629 t
ORIGIN      
        1 tccagcgatc tggcgcagat tgaatcggcg ctgaacgaga tgattgccaa caatcaggac
       61 cgcgaagcgt ttaacgaagc ggatattcgc taccacgagg cggtgctgca gtcggtgcat
      121 aacccggtgt tacagcaact tagcattgcg atcagttcgc tgcagcgggc ggtttttgaa
      181 cgaacctgga tgggcgatga ggccaacatg ccgcaaacgc tccaggaaca taaggcgctg
      241 ttcgatgcga tacggcatca ggacggcgat gcggcagagc aggcggcgct taccatgatc
      301 gctagctcga cacgaaggtt aaaggaaatc acatgacagc tcgctacatc gcaattgact
      361 ggggatcgac caatctgcgc gcctggcttt atcatggcga ccactgcctg gagagcaggc
      421 aatcagaagc aggcgtcacg cgtctgaacg gaaaatctcc ggctgcggtg ttagcagaag
      481 tgacgaccga ctggcgtgaa gagaatacgc cagtggtaat ggcaggaatg gtcggcagca
      541 atgtcggctg gaaagttgca ccgtatttat ccgttcctgc ccgtttttcg tctattggcg
      601 aacaattaac gtcggttggc gacaatatct ggattattcc cggattatgc gtctctcatg
      661 acgataacca taatgtgatg cgcggcgaag aaacacaatt gatcggcgcg cgtgctctgg
      721 ctccttcctc tctttatgtc atgcccggta cgcattgcaa atgggtgcag gccgatagcc
      781 agcaaatcaa cgattttcgc accgtgatga ccggtgaatt acatcattta ctgttaaatc
      841 actcattgat tggcgcaggt ttgccgccgc aggaaaactc tgccgatgcc ttcgcggctg
      901 gtcttgagcg tggccttaat gctcccgcca tattgccgca gatttttgaa gttcgcgcct
      961 cgcatgtgct gggaacactt ccccgagaac aggtcagcga atttctctct ggtttgttga
     1021 ttggcgcaga ggttgccagt atgcgcgact atgtggccca tcaacacgcc atcacccttg
     1081 tcgccggaac atcgctgacc gcgcgctacc agcaagcctt tcaggcgatg ggttgcgatg
     1141 tggcggcggt ggcgggcgac acggcatttc aggctggtat aaggagcatc gctcatgcag
     1201 tggcaaacta aactcccgct gatcgccatt ttgcgcggca ttacgcccga cgaggcgctg
     1261 gcgcatgttg gcgcggtgat tgacgccggg ttcgacgcgg ttgaaatccc gctgaattcc
     1321 ccacaatggg agcagagcat tcccgccatc gttgatgcgt atggcgacaa ggcgttgatt
     1381 ggcgcaggta cggtactgaa acctgaacag gtcgatgtgc tcgccaggat gggctgccag
     1441 ctcatcgtta cgcccaatat ccatagtgaa gtgatccgcc gtgtggtggg ctacggcatg
     1501 accgtttgcc ccggctgcgc gacggcgacc gaagccttta ccgcgctcga cgcgggtgcg
     1561 caggcgctga aaatattccc ttcatcggct tttggtccgc aatacatcaa agcgttaaaa
     1621 gcggtattac caccggacat cgcagtcttt gccgttggcg gcgtgacgcc agaaaacctg
     1681 gcgcagtgga tagacgcagg ttgtgcaggg gcaggcttag gcagcgatct ctatcgcgcc
     1741 gggcagtcca tagaacgcac cgcgcagcag gcagcagcat ttgttaaggc gtatcgagag
     1801 gcagtgcaat gaaaatcacc aaaattacca cgtatcgttt acctccccgc tggatgttcc
     1861 tgaaaattga aaccgatgaa ggcgtggtcg gctggggcga gcccgtgatc gaaggccgcg
     1921 cccgtacagt ggaagcggca gttcacgaac tgagcgacta tttgattggt caggatccat
     1981 cgcgcatcaa tgacttatgg caagtgatgt atcgcgccgg attttatcgt ggcggtccaa
     2041 tcctgatgag cgctatcgcc gggatcgacc aggcgttatg ggatatcaaa ggcaaagtgc
     2101 tgaatacgcc ggtgtggcaa ctgatgggcg gcctggttcg cgacaaaatt aaagcctaca
     2161 gttgggtcgg cggcgatcgt ccggcggatg ttatcgacgg cattaaaacc ctgcgcgaaa
     2221 tcggcttcga taccttcaaa ctgaacggtt gtgaagaact ggggctaatt gataactccc
     2281 gcgcggtaga tgcggcagtc aacaccgtgg cacaaattcg tgaagctttt ggcaatcaga
     2341 ttgagtttgg tcttgatttc cacggtcgcg tcagcgcgcc gatggcgaaa gtgctgatta
     2401 aagaactgga gccgtatcgc ccgctgttta ttgaagagcc ggtgctggcg gaacaagccg
     2461 aatactaccc gaaactggcg gcacaaacgc atattccact ggcggcgggt gaacgcatgt
     2521 tctcacgctt cgattttaaa cgcgtgctgg aggcgggcgg tatttcgatt ctgcaaccag
     2581 atctctctca tgcaggcggt attaccgaat gctacaaaat tgccggaatg gcagaagcct
     2641 atgacgtgac ccttgcgccg cactgtccgc tcggaccgat tgcactggcg gcttgtctgc
     2701 atatcgactt tgtttcctat aacgccgtac ttcaggaaca aagtatggga attcattaca
     2761 acaaaggcgc ggagttactc gactttgtga aaaacaaaga agacttcagc atggtcggcg
     2821 gtttctttaa acca
//
LOCUS       DAOTRK010000276         2807 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.279, whole genome shotgun sequence.
ACCESSION   DAOTRK010000276 DAOTRK010000000
VERSION     DAOTRK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.279"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            45..1640
                     /locus_tag="QIE41_004692"
     CDS             45..1640
                     /locus_tag="QIE41_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDS3533461.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNQEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            1654..2805
                     /locus_tag="QIE41_004693"
     CDS             1654..2805
                     /locus_tag="QIE41_004693"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDS3533462.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
BASE COUNT          747 a          659 c          730 g          671 t
ORIGIN      
        1 tccatctggc gtagggaaaa tcacatctga atcaggaatt aacaatgaaa cctgtaaaac
       61 cacctcgtat taatggacga gtgccggtcc tgtcggcaca ggaagcggtg aattatattc
      121 ccgacgaagc aacactttgt gtgttaggcg ctggcggcgg tattctggaa gccaccacgt
      181 taattactgc tcttgctgat aaatataaac agactcaaac accacgtaat ttatcgatta
      241 ttagtccaac agggcttggc gatcgcgccg accgtggtat tagtccgctg gcacaagaag
      301 gtctggtgaa atgggcatta tgcggtcact ggggacaatc gccgcgtatt tctgatctcg
      361 cagaacaaaa taaaattatt gcttataact atccacaagg tgtacttaca caaaccttac
      421 gcgccgccgc agcccaccag cctggtatta ttagtgatat tggcatcggg acatttgttg
      481 atccacgcca gcaaggcggc aaactgaatg aagtcactaa agaagacctg attaaactgg
      541 tcgagtttga taaccaagaa tatctctatt acaaagcgat tgcgccagat atcgccttca
      601 ttcgcgctac cacctgcgac agcgaaggct acgccacttt tgaagatgag gtgatgtatc
      661 tcgacgcatt ggttattgcc caggcggtgc acaataacgg cggtattgtg atgatgcagg
      721 tgcagaaaat ggttaagaaa gccacgctgc atcctaaatc tgtccgtatt ccgggttatc
      781 tggtggatat tgtggtggtc gatccggatc aaacccaact gtatggcggt gcgccggtta
      841 accgctttat ttctggtgac ttcacccttg atgacagcac caaacttagc ctgcccctaa
      901 accaacgtaa attagttgca cggcgcgcat tattcgaaat gcgtaaaggc gcggtgggga
      961 atgtcggcgt cggtattgct gacggcattg gcctggtcgc ccgagaagaa ggttgtgctg
     1021 atgactttat tctgacggta gaaacaggtc cgattggcgg aattacttca caggggatcg
     1081 cctttggcgc gaacgtgaat acccgtgcca ttctggatat gacgtcccag tttgattttt
     1141 atcacggtgg cggtctggat gtttgttatt tgagttttgc tgaagtcgac cagcacggta
     1201 acgtcggcgt gcataaattc aatggtaaaa tcatgggcac cggtggattt attgatatca
     1261 gtgccacttc gaagaaaatc gttttctgcg gcacattaac tgcgggcagt ttaaaaacag
     1321 aaattaccga cggcaaatta aatatcgtcc aggaaggacg ggtgaagaaa tttattcggg
     1381 aactaccgga aattactttc agcggaaaaa tcgctctcga gcgagggctg gatgttcgtt
     1441 atatcactga gcgcgcagta ttcacgctga aagaagacgg cctgcattta atcgaaatcg
     1501 cccctggcgt cgatttacaa aaagatattc tcgacaaaat ggatttcacc ccagtgattt
     1561 cgccagaact caaactgatg gacgaaagat tatttatcga tgcggcgatg ggttttgtcc
     1621 tgcctgaagc ggctcattaa caggagtatc acgatggatt tttctttaac tgaagaacaa
     1681 gaactgctgc tggccagtat tcgcgaactg attacgacta actttccgga agagtatttc
     1741 cgcacctgcg atcaaaacgg gacatacccg cgtgaattta tgcgggcact ggcggataac
     1801 ggtatttcca tgcttggcgt gccggaagaa tttggtggta tccctgcgga ttacgtcacc
     1861 caaatgctgg cgctgatgga agtgtcaaaa tgcggtgccc cggcattttt gatcaccaac
     1921 ggtcaatgta ttcacagtat gcgccgtttc ggttctgcag agcagctacg taaaacggca
     1981 gagagcaccc tggaaacggg tgatcccgcc tatgccctgg cattgacgga accaggcgca
     2041 ggctcagata acaacagcgc caccaccact tacacgcgta aaaacggcaa ggtttacatc
     2101 aacggccaga aaacctttat taccggcgcg aaagagtacc cgtatatgct ggtgctggcg
     2161 cgcgatccag aaccgaaaga tcccaaaaaa gccttcaccc tgtggtgggt cgactccagt
     2221 aagcccggca ttaagattaa cccgctgcat aaaatcggct ggcatatgct cagcacctgc
     2281 gaagtctatc tcgacaacgt ggaagttgaa gagagcgaca tggtgggcga agaaggaatg
     2341 ggtttcctca atgtgatgta caactttgag atggagcgcc tgatcaacgc cgcgcgcagc
     2401 accggctttg ccgagtgtgc ctttgaagat gccgcccgct atgccaacca acgtatcgct
     2461 tttggtaagc ccattggtca taaccagatg atccaggaaa aactggcgct gatggcgatt
     2521 aagatcgaca acatgcgcaa catggtgctg aaagtggcat ggcaagccga tcagcatcag
     2581 tcactgcgca ccagcgcggc gctggcaaaa ctgtactgcg cacgtaccgc aatggaagtt
     2641 attgatgatg cgattcaaat catgggcggt ctgggctata ccgatgaggc gcgcgtctct
     2701 cgcttctggc gtgatgtccg ttgtgaacgt atcggcggcg gtacagacga aattatgatt
     2761 tacgtagcgg gtcggcagat cctgaaagat tatcagaaca aataatc
//
LOCUS       DAOTRK010000277         2738 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.280, whole genome shotgun sequence.
ACCESSION   DAOTRK010000277 DAOTRK010000000
VERSION     DAOTRK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.280"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            827..1603
                     /locus_tag="QIE41_004694"
     CDS             827..1603
                     /locus_tag="QIE41_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS3533463.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISKRSVSAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVSVYILLMLIVRMAGEYFEITNFLQLVATWSLLIILSCSVGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLITLFIGLALYRTREEAMLTS"
     gene            1600..2271
                     /locus_tag="QIE41_004695"
     CDS             1600..2271
                     /locus_tag="QIE41_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS3533464.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNLEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVALFIPRDHQTCLYQNINDAIKLYQND
                     ENLNI"
     gene            2283..2678
                     /locus_tag="QIE41_004696"
     CDS             2283..2678
                     /locus_tag="QIE41_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDS3533465.1"
                     /translation="MRKVITFGTFDVLHIGHINILKRAKKMGDYLIVGVSSDYLNFSK
                     KQRYPVYPETERLEIIRSLKFVDEVFIEESLELKGEYIKKFKADILVMGDDWLGRFDE
                     YKELCEVSYLPRTVGISTTELISQIKRYG"
BASE COUNT          862 a          451 c          588 g          837 t
ORIGIN      
        1 agaaagggaa tttcaaataa gcataataat ttccagtgaa actattcgtc gattttaaga
       61 aatgtctgct caggtatatc tactgaggga tggtgttggt tgtaacactg gttaaaataa
      121 acacacaaaa ataatcaaga tgtatatttt aatcgacaaa aataattact cgcggaatta
      181 ttgatgggtg ttaaataaca ccgtttaaat gtgatgtaaa tcacaaatgt gactgtaaag
      241 agggtgctgt agatataaat aagaagtaca tgagtgaatt ttaaataggg aaatagtttc
      301 ttgctaaaca atttattggt aatcaatcgc atgcgttctg gtttgagata gattacaggt
      361 gttactacgc ataaaattca tgggtggtat tataatcaag tagttaacag taatagtgaa
      421 aaataatttc ttgcgaaatt agcggcccgt tttgaataag tgtgttccgg agtaatccag
      481 aacgcatgct gctttacatc tttttcaagc catattggtt aagcaaaaag aagtagaaat
      541 aatttcctga gaaattaact caacattctg aaacggccag ttttctgaaa ttaccagaag
      601 accgaataca ttcatgcctg aagaatggat tagaagaaac gagacggaaa tagatctatt
      661 tatccctgcg gaaataattt ctgctgaaat tgtttgcggc tattaaaaag gtcaaaccgt
      721 ctgactaaat tttatccagt tacaaacaag tattacctcc agtgtattgg tagctgttaa
      781 gccaggggcg gtagcatacc tgaagagaat aggatcacat caccaaatgg caagaagtgg
      841 atttgaagtc cagaaagtca ccgtagaggc attatttcta cgagaaatac gaacacgctt
      901 tggtaagttc cgtctgggat atctgtgggc gattctcgaa ccctctgcgc atttgctgat
      961 actgttgggc atttttggtt acattatgca ccgcacgatg ccagacatct cgttcccggt
     1021 gtttttactt aatggcctga ttcccttttt tatctttagc agtatcagca aacgttctgt
     1081 aagtgctatt gaagcgaacc aggggctgtt taattatcgc ccagtaaaac ccatcgatac
     1141 gatcattgcg cgcgccctgc ttgagacgct gatttacgtt tctgtttata ttctgcttat
     1201 gctcattgtc aggatggcag gcgaatattt cgagataaca aattttttac aacttgtggc
     1261 tacctggagt ctgctgatca ttctttcatg cagcgtcggc ttaatattca tggtcgttgg
     1321 taaaaccttc cccgaaatgc aaaaggttct gccgatactt cttaagcccc tttatttcat
     1381 ctcctgcatc atgttccctc tgcactcaat tccaaagcaa tactggtcat atctactctg
     1441 gaacccacta gtgcatgtcg tggagttaag ccgtgaggcc gttatgcctg gctatattag
     1501 cgaaggtgtg agtctgaact acctggcaat gttcacattg ataactctgt tcattggtct
     1561 ggcgttatat cgaactcgtg aggaggcaat gctgacatca tgattaagat tgagaatttg
     1621 acgaagtcat atcgcacgcc aacgggtcga cattatgtgt ttaaggattt aaacctcgaa
     1681 atcccttcag gaaaaagtgt tgcctttatt ggtcgtaatg gtgcgggtaa atcaacgtta
     1741 ctgagaatga ttggtggcat tgaccgcccc gacagtggaa agatcatcac taataaaaca
     1801 atatcatggc cagtcggcct tgcaggtgga tttcagggaa gtttaaccgg acgcgaaaac
     1861 gtaaaatttg ttgcgcggtt atacgcgaag caagaagaac tgaaagagaa aattgagttt
     1921 gttgaagaat ttgccgaact tggcaagtac ttcgatatgc cgattaaaac ttactcatca
     1981 ggtatgcgat cgcgtttagg ctttggttta agcatggcat ttaagtttga ttattatatc
     2041 gtcgatgaag taacggcggt cggtgatgcc aggtttaaag aaaaatgcgc tcaattgttt
     2101 aaagaaaggc ataaagaatc tagcttttta atggtttcac atagtttgaa ttcattgaaa
     2161 gagttttgtg atgtggcgct attcatacct agagatcatc agacttgtct ttatcagaat
     2221 attaatgacg caattaagtt atatcaaaat gatgaaaatt tgaacattta gtggagctaa
     2281 aaatgcgtaa agttatcacc tttggtacat ttgatgtatt acatataggt catataaata
     2341 tcttaaaaag ggcaaaaaaa atgggggact atctaatagt tggcgtctca tccgattatt
     2401 tgaatttctc aaaaaaacag aggtatcctg tttatccgga aactgagcgt cttgaaataa
     2461 ttaggtcctt aaaatttgta gatgaagttt ttattgaaga gtcattggaa ttaaagggcg
     2521 agtatattaa aaaatttaag gcagatatct tagtgatggg agatgattgg ctagggcgat
     2581 ttgatgaata taaggaatta tgtgaagtat catatctgcc aagaactgtt ggtatttcaa
     2641 cgacagaact aatttctcaa atcaaaagat atggttaatt ttggagaggg attttgaaat
     2701 ttcattcaga gataaaaaac tctataaaaa atgcgaag
//
LOCUS       DAOTRK010000278         2715 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.281, whole genome shotgun sequence.
ACCESSION   DAOTRK010000278 DAOTRK010000000
VERSION     DAOTRK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.281"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1112
                     /locus_tag="QIE41_004697"
     CDS             <1..1112
                     /locus_tag="QIE41_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDS3533466.1"
                     /translation="KELRKGADESETLRQLEALQKNRGEKPVRYKFIFNDATTAAIKD
                     QLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNEPEQMIENARMT
                     LSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGAVTPGGSLTAFH
                     ERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSGPLSQFRGHVSK
                     KTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFALPDESELQLQDA
                     RELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILASQLRLSVVQEGK
                     TTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(1233..1805)
                     /locus_tag="QIE41_004698"
     CDS             complement(1233..1805)
                     /locus_tag="QIE41_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533467.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(1891..2097)
                     /locus_tag="QIE41_004699"
     CDS             complement(1891..2097)
                     /locus_tag="QIE41_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDS3533468.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARITQSRP"
BASE COUNT          725 a          604 c          711 g          675 t
ORIGIN      
        1 ataaagaact ccgcaaaggc gcggatgagt cagaaacgct cagacaactg gaagcgcttc
       61 agaaaaacag aggggaaaaa cctgtaagat ataaattcat ttttaatgat gccacgacag
      121 cagcaattaa agatcagctt tgtggtcagt ggcgttcagt gggtatcatg tctgatgaag
      181 ccggtataat atttgatggg tataccctgt ctgagctgcc ttttattaac aaaatgtggg
      241 atggctccgt attgtcggtg gacagaaaga atgaacccga acagatgatt gaaaatgcca
      301 gaatgacatt atccctcatg gtacaacccg gtctttttga ccgatatatg gagcgaaaag
      361 gttctgttgc cagggacagt ggttttcttg cccgttgtct aatcagtaaa cctgccacca
      421 cgcaggggaa acgctttatt aacggagctg tgaccccggg aggatcatta acggcatttc
      481 atgaacgatt aatggaactg gccagaggca gtattgaaaa atccagtaag gatgaacgtt
      541 attgtctgca tttttctcct gaagcgcaaa aaatatttat tgatcactat aatgttctgg
      601 agcaggattt gtctccttcc ggacccttaa gccagtttcg gggacatgtt tcaaaaaaaa
      661 cagaaaacat tgcgagaatt gctgcgttat tgcagtattt cagtcagggt gaagggaaaa
      721 tctctgccga tatcatgacg tctgctgtcg taataagttc gtggtacaca gatgaatata
      781 aaaaattatt tgcgttacct gatgaatctg aactgcagct gcaggacgcc cgggagctgc
      841 tcgactggct cattgaggag tgccgcggag aatgcccgcc ccgtgtcagg aaaaattata
      901 ttctccagtg tggcccgggc cggttccgga acaggaaaaa gctgaacgtc ctgctgaaca
      961 ttcttgcatc tcaactcagg ctctctgtcg tgcaggaagg aaaaaccacc tatgtcctgc
     1021 tttccgaaat aaacaatatc acgctgcctg aactgaatgc gtgctacagc cagaaactga
     1081 taagatggca tgagcaatcc tttggcttat aatgttccct gttgctgaga cggattattc
     1141 agtggtggta tcgcagtatg cccgtcaccc ccgcttctgc atgagggtga cggaggaaaa
     1201 gtaagtgtgc ggaggcccgg gagcgttatg aatcaacagg cgtataacag gtactcgtca
     1261 atcatcagtt ccagctcgcg cttttcccgt ttgcgcgcca cagcccagtt ttcaagctct
     1321 gcatgtgatt tatgctttaa ctcttcagtc cagggctgca tggagtgggc acacatcaga
     1381 tcataccagc acagccatat cagcgtgtgg tagagatcac gatcgtcgaa acgccggagc
     1441 aggctgttca gcctgtttgc cagccggttt tcacccagac gatgtagctc actgagtaaa
     1501 acgccgccca gattaaaatg tgtaagcagc atgatttcat gggtctgaat gctggtcatt
     1561 aacccttcct gaatggcagg aatgaacggt ggtgttcccg ggttgacgac ctgatagagt
     1621 ccctgtggtg ccactgagat gatggtgcct ttttctggca gaaaaaagtg ggccagggtc
     1681 aggcggtgat aaccatgaag atgcggagcc cccattccat gagcgacgat aagcctcgtc
     1741 acgtttgtca tccatttctg atgctggatg agcagcagct cacaccggtt ttcaagtttt
     1801 tgcataatat tctctccttt agcgcgcagc aaaatgcctc cgggggccgg atcatgcctt
     1861 tgctgcggtt gaacggataa cggtcaggaa ttacggacgg gactgtgtaa tacgtgcctg
     1921 caaccaggct tccacttcac ttttcagcca tcgggagcta cggcccagtt tgatgggggc
     1981 aggaaaggca ccatccttga tgagcttgta aaaccattta tcggtcaggc cggtcagctg
     2041 agtgataaac gacatgtcga ccatctggtc atccatcagt gaaactgggg tattcatgaa
     2101 aatgttcctc cggatgttgt taatctcaac cggcgttggt gatgaaaaca acgcaccacc
     2161 ttccccggca taacaaccgg ggaaaaacgt aaaaaataat cgggaatgtg accgtcagga
     2221 gataaacggg gggattgcgg tggctgagcc gtaccggtat gtcgggtgat tgttgcgaaa
     2281 cgatgcttcc gcacgcgcac gtgataaagg agggaggact ttcgtgagac aaggggaaaa
     2341 ctggcgttct gataattgca gagttgtttt tgaaggcggt tgtggtgcat cagcccgtga
     2401 cctgtgggca ggcgacaggt atcactggtg ggataagact gtctgattgg tattataaat
     2461 atataagaga gctcaaacac atgagttcat atatgtgaaa taaatctgtg agtgtgatca
     2521 gcagaccagt ctacccagta gcaacagatt gcgttgtact cacagggtta tccctgataa
     2581 ctggcgtata acctgcgtac ataaaacgta cctgcaatgc acctgaacag agtgcattaa
     2641 gcatccgcct gagcggtaaa cgtatccgat ctggtgagtg acagtctgag agcattctca
     2701 gtaatcgccc ccttg
//
LOCUS       DAOTRK010000279         2688 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.282, whole genome shotgun sequence.
ACCESSION   DAOTRK010000279 DAOTRK010000000
VERSION     DAOTRK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.282"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..63
                     /locus_tag="QIE41_004700"
     CDS             <1..63
                     /locus_tag="QIE41_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein bor"
                     /protein_id="HDS3533469.1"
                     /translation="LGFITLGIYTPLEARVYCSQ"
     gene            complement(154..336)
                     /locus_tag="QIE41_004701"
     CDS             complement(154..336)
                     /locus_tag="QIE41_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HDS3533470.1"
                     /translation="MRKLKMKLCALMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPAP
                     DWQTPLNGIISPSERG"
     gene            complement(553..1086)
                     /locus_tag="QIE41_004702"
     CDS             complement(553..1086)
                     /locus_tag="QIE41_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDS3533471.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVLLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIEQ"
     gene            complement(1150..1500)
                     /locus_tag="QIE41_004703"
     CDS             complement(1150..1500)
                     /locus_tag="QIE41_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDS3533472.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIVDLDISLDR
                     VEGAALEFYEAAARMSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            complement(1505..1720)
                     /locus_tag="QIE41_004704"
     CDS             complement(1505..1720)
                     /locus_tag="QIE41_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HDS3533473.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(1870..2031)
                     /locus_tag="QIE41_004705"
     CDS             complement(1870..2031)
                     /locus_tag="QIE41_004705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533474.1"
                     /translation="MILRHRTGPAFQAIKDGLAVGLNALTLTDITKNATYGVEMESLV
                     LEINAPASS"
     gene            complement(2028..2231)
                     /locus_tag="QIE41_004706"
     CDS             complement(2028..2231)
                     /locus_tag="QIE41_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HDS3533475.1"
                     /translation="MVERTMAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            2477..>2688
                     /locus_tag="QIE41_004707"
     CDS             2477..>2688
                     /locus_tag="QIE41_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDS3533476.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKVKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKT"
BASE COUNT          687 a          716 c          556 g          729 t
ORIGIN      
        1 ctcggtttta ttactttagg catttatact ccactggaag cgcgtgtgta ttgctcacaa
       61 taattgcatg agttgcccat cgatatgggc agctctatct gcactgctca ttaatatact
      121 tctgggttcc ttccagttgt ttttgcatag tgatcagcct ctctctgagg gtgaaataat
      181 cccgttcagc ggtgtctgcc agtcgggggc tggttgcatt atccacgccg gaggcggtgg
      241 tggcttcacg cactgactga cagactgctt tgatgtgcaa ccgacgacga ccagcggcaa
      301 catcagcgcg cagagtttca ttttcagctt tcgcattggc taattctctc gagtactttg
      361 catcgagtgc agcaacatca cgctgacgct gctgcatgtc agcgatggtg gcgatcgact
      421 gcttcagctc actgactttt ttatcacgct gttctttgta ggcgatggcg ttatcacggt
      481 aatgattgac cgcccacgac aggcagacga tgatgcagat aaccaaagca taaataatcg
      541 cggcgactct gctcactgct ctatccccca acaggctaat gcgctttcct ggtcacgacg
      601 aataacctgt ccatagcagt tatttgaacg tatgcggcaa tcgcgcccac catcttttat
      661 ccaccagcga atcgcctcgc atgcgccctt acgatcaccg gcattcagcc gcttataaaa
      721 cgtcgacggg aaacacttac cggggccaat gttataggga cagaatgacg ctatacccgc
      781 tttttgtggt tcggtcagta gtactttaat attgcgctcc acccatgcca gcgccttatc
      841 acgctcaatg gcgttgacct ggtcgcattt ttccttcgac agtttcatac cgggaaaaac
      901 gggttttcca tccaccatcg tggcaccccg acagatggtc cagatgccgg acccatcgcg
      961 gtatgccgtt gtgtggttac cttctttttc atccagaaac tggtcgagaa tatcaggcgc
     1021 gggcgcaccg acggcaatca gtgccagaac ggcagccgac aggccgtatc tgatttttgc
     1081 gttcatggat atttatcagg atttatcggt ttctgcccac ggacaggttt atctgttccg
     1141 gtcagtgact taaggttgtg attccggagg agtcttcaga gaaccagtaa ttcttcccgg
     1201 tagctttcct ttgtaggtta tccatacatt ctgcgcctct aaaattacgg ggcgcttttc
     1261 cggcgactgc tcatcccctt cacataaccc ggcagcaaca tccaggaaga cctgtctgat
     1321 gctcattctg gctgctgcct cataaaactc cagcgcggca ccttcaacac ggtccagcga
     1381 gatgtccagg tcaacaattt caccgtcaaa gcgttttttg tcccgtaacg ctaaagttac
     1441 cgtaacttta ttctcaaaat tgcggatccc tttcacaatc agttcatagt tttgagtcat
     1501 tgaattactc tccccgtgca gccttacgac ggtcctctct gattttgaaa tacaggttag
     1561 tcagatatgt cagcagccca aacagcagac tccccagcac gcctattgcc gcccactgag
     1621 acggggaaac cctgtccagc aactgcagga accagtagcc cgttcccacc gctgacgtgg
     1681 tgtatgacac acctgttgtg attttttcca tctggtacat accccgtctc ccgttatccg
     1741 gaagctgaca acaataaaaa agccaccagt taagtactga tggctctgat aactcatgca
     1801 ggcgtctcag acgacccact gacactaccg gtgagtttaa cgataccttc catttgactg
     1861 gctcactttt tatgatgatg ccggtgcatt tatctccagc accagacttt ccatctcaac
     1921 gccatacgtt gcatttttgg taatatccgt cagcgtcagt gcatttagtc ccactgccag
     1981 accgtctttt atggcctgga atgccgggcc agtacgatga cgtagtatca ctccggctca
     2041 gttgcaccac tgaccaccac atcaccttct gctgcaatcg cctgcatcag ggtataaggg
     2101 gttatggcca ccggactacc aaacggctgc cagccctctt tcagtttatg tgtcagcttt
     2161 tccgcaagat ctgacggcga cgccgccctg acaacatcat agtgtttaaa tgccatggtt
     2221 ctttccacca tctgaaaaat gattctttaa aatacctgac atgtaataca gaaaaaacac
     2281 aaaaccatac cttaattaaa aacctcatca tcaagcagat atgcatggat aaactacaag
     2341 acgagatata aaccaccctg catttaaata aacaataaac aacatcagaa aaataattct
     2401 gctctatggt ttatattcaa aaatatcatt tatacttttc agaacatcac cagcaaggca
     2461 taaacaagga agccaaatga agtggattgt aattgataca gttatccagc catcatgcgg
     2521 aatatctttt tcagtcatat ggagtaaagt aaaattaata atctggtatc aatcggatgc
     2581 tttcttacct cctgaaagta tatttacact gactcacaca ggtatcatgc tcaataacaa
     2641 agtgctacct gtaaccattt acaacgtagt accattcaat aaaacatt
//
LOCUS       DAOTRK010000280         2652 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.283, whole genome shotgun sequence.
ACCESSION   DAOTRK010000280 DAOTRK010000000
VERSION     DAOTRK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.283"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            179..2176
                     /gene="betT"
                     /locus_tag="QIE41_004708"
     CDS             179..2176
                     /gene="betT"
                     /locus_tag="QIE41_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDS3533477.1"
                     /translation="MSESDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMVDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2239..>2652
                     /locus_tag="QIE41_004709"
     CDS             2239..>2652
                     /locus_tag="QIE41_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HDS3533478.1"
                     /translation="MISRWKWMLKQTFKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYEERGRVILFIV"
BASE COUNT          602 a          635 c          671 g          744 t
ORIGIN      
        1 caacctcccc ccctaaaaac ctctttccga tgccactcta caacccccta aaaaaccggt
       61 ggcgattcac attccaccat gcattccgat taacgccgca tagccagttg aactttgcta
      121 ctctgtgaga ggtagtacct tctatccagt gcgaatttaa ttaatggaat aaatgattat
      181 gagtgaaagt gatacaatcc caaagaagtc tacaagtcag attaacaaag cggtattctt
      241 tacatctgct ttgctaattt tccttcttgt cgcctttgcc gccgtattcc cggatgtcgc
      301 cgacaaaaat tttaaactac ttcagcaaca aatcttcacg aatgccagct ggttctacat
      361 ccttgctgtg gccctgattt tactgagtgt cacgttcctt ggactctcac gctacggtga
      421 tatcaagcta ggcccggacc atgcgcagcc tgatttcagc taccactcct ggtttgcgat
      481 gcttttttcg gcagggatgg ggatcggcct gatgttcttt ggcgttgccg aacctgtaat
      541 gcattatctt tcgccacccg ttggcactcc agaaaccgtt gcggcagcca aggaagcaat
      601 gcgtctgacc tttttccact ggggactgca cgcatgggca atttatgcca ttgtggcgct
      661 gattctggcg ttcttcagtt accgtcacgg tctgccttta actctgcgct ccgcactcta
      721 tcccattatt ggcgatcgca tatacggacc tgtaggacat gcggttgata ttttcgctgt
      781 tataggcacg gtctttggcg ttgcgacatc actgggttac ggtgttttgc aggtgaatgc
      841 cggtttgaac catcttttcg gggtgcccat caatgaaacg gtgcaggtaa ttctgatcgt
      901 ggtcatcacg gggttagcga cgatttcagt ggtgtccggt ctggataagg gaatacgtat
      961 cctgtctgaa ctcaatctgg gtctggcttt gttgctcctg gcgctggtcc tgtgtctggg
     1021 accaaccgtg cttctgctga agtcatttgt ggaaaatacg ggcggttatc tttcggaact
     1081 ggtgagtaaa acgttcaacc tttacgcgta tgagcccaag tcgagcaact ggctgggggg
     1141 ctggacatta ctgtactggg gatggtggct ttcatggtcg ccgtttgtgg ggatgttcat
     1201 cgcacgggtc tcccgcgggc gaaccattcg cgagtttgtc accggcgtgc tgtttgttcc
     1261 cgcgggtttt acgctaatgt ggatgacggt gtttggtaac agcgcgatct atctcattat
     1321 gaaccagggg gccacagacc tcgccaatac tgttcagcag gatgtggcgc tggccctgtt
     1381 taatttcctg gagcatttcc cgttctcttc cgtgctgtca ttcattgcaa tggcgatggt
     1441 catcgtcttc tttgtaacgt ctgctgattc gggggcaatg gttgtggata ctctggcatc
     1501 aggtggagtg gcaaacacac ccgtctggca gcgaatattc tgggcctcgc tcatgggcat
     1561 tgttgcaatt gcgcttctcc ttgccggagg gctaagtgcg ctgcaaacgg tgacaatagc
     1621 gagtgcattg cccttctcag tgatcttact aatatccata tacggacttt taaaagcttt
     1681 gcgccgggat ttgaccaagc gtgaaagcct gagcatggcg acaattgctc ctacggctgc
     1741 acgtaaccca attccttggc agagaaggtt acgcaatatc gcgtatctgc cgaagcgatc
     1801 tcttgtgaaa cgttttatgg tcgacgtcat ccagcccgcc atgacgctgg ttcaggagga
     1861 actgaacaag caggggacga taagccacat tagtgatgca gtcgacgatc gtattcgtct
     1921 tgaagtcgat ttgggcaacg agctgaattt tatatatgaa gtgaggcttc gcgggtatat
     1981 ctcaccgacc ttcgcgctcg ccgcaatgga taatgatgag cagcagactg aacaacatcg
     2041 atattatcgc gctgaggttt atctcaaaga aggcggtcaa aattatgatg tgatgggctg
     2101 gaaccaggaa cagctgatta atgacatact ggaccagtac gaaaaacacc tgcacttcct
     2161 gcacctggtt cgttaatagc aacatgccgt cctgggggcg gcaattatta tcccggccgc
     2221 aatatgaggg aatgcagaat gatttcacgc tggaaatgga tgctgaagca gacatttaaa
     2281 aaactatggt tcagggcaac gttattcgca attgtcgcga taataacggc ccttttatca
     2341 attcttttta aatcaatgat acctgagtcg gtttccgtga aggttggtgc ggaagcagtc
     2401 gataacattc tgaacatact ggcatcgagt atgctggcag tgaccacatt ttcgctgagt
     2461 atcatggtca cagcctacgg ttcagccact actaatgtga ctcccagagc tacgcgttta
     2521 gttgttgaag acgtcaccac acaaaatgta ttggccacct tcatcggttc ttttctcttc
     2581 agtctggtag ggattattgc cctcaatatg ggagcttatg aagaaagggg gagagtcatt
     2641 ttattcattg tc
//
LOCUS       DAOTRK010000281         2603 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.284, whole genome shotgun sequence.
ACCESSION   DAOTRK010000281 DAOTRK010000000
VERSION     DAOTRK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.284"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..561
                     /gene="tolA"
                     /locus_tag="QIE41_004710"
     CDS             <1..561
                     /gene="tolA"
                     /locus_tag="QIE41_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDS3533479.1"
                     /translation="KKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            694..1986
                     /gene="tolB"
                     /locus_tag="QIE41_004711"
     CDS             694..1986
                     /gene="tolB"
                     /locus_tag="QIE41_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDS3533480.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            2021..2542
                     /gene="pal"
                     /locus_tag="QIE41_004712"
     CDS             2021..2542
                     /gene="pal"
                     /locus_tag="QIE41_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDS3533481.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
BASE COUNT          671 a          626 c          708 g          598 t
ORIGIN      
        1 aagaaagcgg cagcagagaa agctgcagcc gacaaaaaag cagcagaaaa agcggctgct
       61 gaaaaggcag cagctgataa gaaagcagcg gcagaaaaag ccgccgcaga caaaaaagcg
      121 gcagctgcaa aagcagcagc tgaaaaagcc gctgcagcaa aagctgccgc agaggcagat
      181 gatattttcg gtgagctaag ctctggtaag aatgcaccga aaacgggggg aggggcgaaa
      241 gggaacaatg cttcgccagc cgggagtggt aatactaaaa acaatggcgc atcaggggcc
      301 gatatcaata actatgccgg gcagattaaa tctgctatcg aaagtaagtt ctatgacgca
      361 tcgtcctatg caggcaaaac ctgtacgctg cgcataaaac tggcacccga tggtatgtta
      421 ctggatatca aacctgaagg tggcgatccc gcactttgtc aggctgcgtt ggcagcagct
      481 aaacttgcga agatcccgaa accaccaagc caggcagtat atgaagtgtt caaaaacgcg
      541 ccattggact tcaaaccgta atcgcgatgt tgactgttcc gacggtcaac atcaggcacc
      601 ggttgccacg gggttctggt agttttgtgt attttagttt gttaacattc tgctaaatta
      661 tcgtgggcca tcggtccaga taagggagat atgatgaagc aggcattacg agtagcattt
      721 ggttttctca tactgtgggc atcagttctg catgctgaag tccgcattgt gatcgacagc
      781 ggtgtagatt ccggtcgtcc tattggtgtt gttcctttcc agtgggcggg tcctggtgcg
      841 gcacctgaag atattggcgg catcgttgct gctgacttgc gtaacagcgg taaatttaat
      901 ccgttagatc gcgctcgtct gccacagcag ccgggtagtg cgcaggaagt acaaccagct
      961 gcatggtccg cgctgggtat tgacgctgta gttgtcggtc aggtcactcc gaatccggat
     1021 ggttcttaca atgttgctta tcaacttgtt gacactggcg gcgcaccggg tactgtactt
     1081 gctcagaact cgtacaaagt gaacaagcag tggctgcgtt atgctggtca taccgccagt
     1141 gatgaagtgt ttgaaaaact gaccggcatt aaaggtgcgt tccgtacccg tattgcctac
     1201 gttgttcaga ccaacggcgg tcagttcccg tatgaactgc gcgtatctga ctatgacggt
     1261 tacaaccagt ttgtcgttca ccgttcaccg cagccgctga tgtctccggc gtggtcacca
     1321 gacggttcta aactggctta tgtgaccttc gaaagcggtc gttccgcgct ggtcatccaa
     1381 acgctggcaa atggcgctgt acgtcaggtg gcttcattcc cgcgtcacaa cggtgcacct
     1441 gcattctcgc cagacggcag caaactggca ttcgccttgt cgaaaaccgg tagtctgaac
     1501 ctgtacgtaa tggatttggc ttctggtcag atccgccagg tgacggatgg tcgcagtaac
     1561 aataccgaac cgacctggtt cccggacagc cagaacctgg catttacttc tgaccaggcc
     1621 ggtcgtccac aggtttataa agtgaatatc aacggcggtg cgccacaacg tattacctgg
     1681 gaaggttcgc agaaccagga tgcggatgtc agcagcgacg gtaaatttat ggtaatggtc
     1741 agctccaatg gtgggcagca gcacattgcc aaacaagatc tggcaacggg aggcgtacaa
     1801 gttctgtcgt ccacgttcct ggatgaaacg ccaagtctgg cacctaacgg cactatggta
     1861 atctacagct cttctcaggg gatgggatcc gtgctgaatt tggtttctac agatgggcgt
     1921 ttcaaagcgc gtcttccggc aactgatgga caggtcaaat tccctgcctg gtcgccgtat
     1981 ctgtgataat aattaattga atagtaaagg aatcattgaa atgcaactga acaaagtgct
     2041 gaaagggctg atgattgctc tgcctgttat ggcaattgcg gcatgttctt ccaacaagaa
     2101 cgccagcaat gacggcagcg aaggcatgct gggtgccggc actggtatgg atgcgaacgg
     2161 cggcaatggc aacatgtctt ccgaagagca ggctcgtctg caaatgcaac agctgcagca
     2221 gaacaacatc gtttacttcg atctggacaa gtacgatatc cgttctgact tcgctcaaat
     2281 gctggatgca cacgcaaact tcctgcgtag caacccgtct tacaaagtca ccgtagaagg
     2341 tcacgcggac gaacgtggta ctccggaata caacatctcc ctgggtgaac gtcgtgcgaa
     2401 cgccgttaag atgtacctgc agggtaaagg cgtttctgca gaccagatct ccatcgtttc
     2461 ttacggtaaa gaaaaacctg cagtactggg tcatgacgaa gcggcatact ccaaaaaccg
     2521 tcgtgcggta ctggtttact aagagaattg catgagcagt aacttcagac atcaactatt
     2581 gagtctgtcg ttactggttg gta
//
LOCUS       DAOTRK010000282         2572 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.285, whole genome shotgun sequence.
ACCESSION   DAOTRK010000282 DAOTRK010000000
VERSION     DAOTRK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.285"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..208)
                     /locus_tag="QIE41_004713"
     CDS             complement(<1..208)
                     /locus_tag="QIE41_004713"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="catalyzes the formation of 2-oxobutanoate from
                     L-threonine; catabolic; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDS3533482.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSL"
     gene            complement(307..1245)
                     /gene="tdcA"
                     /locus_tag="QIE41_004714"
     CDS             complement(307..1245)
                     /gene="tdcA"
                     /locus_tag="QIE41_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDS3533483.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            1434..1778
                     /gene="tdcR"
                     /locus_tag="QIE41_004715"
     CDS             1434..1778
                     /gene="tdcR"
                     /locus_tag="QIE41_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDS3533484.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINNAARHIFSRYLLKNKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRMSFCECNRFP"
     gene            2034..>2572
                     /locus_tag="QIE41_004716"
     CDS             2034..>2572
                     /locus_tag="QIE41_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533485.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPTYFNKTSIP
                     SDCWQILFFSPDHFNNDFY"
BASE COUNT          823 a          522 c          463 g          764 t
ORIGIN      
        1 tcagtgaact taatttatta aatgcgccac gaattttaaa tgaaccggta cgctgcatgt
       61 tttcgaattt gaggaatatt tcacctttgc aacgttcact aaaatagttg gagcgaggca
      121 tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaatgatg tcatcaatag
      181 caaccgggag atcgtatgta atatgcatta taaaacctct tagcctgaat taaatatgta
      241 ggtaaccgta accgacacct gcaaaacaga caggtggatt atttatgttt aaataaaaac
      301 aaataactaa ccaacttcaa ttaattgcct tcgtctacac ccattataag atgaatactc
      361 tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt tcgaccatac
      421 cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga atagtaataa attgatttga
      481 accaaaaggt gatgtcatat cacaaggaat tacagttaag aaatcagcat tgagaacaag
      541 attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga tgccatttct
      601 ttgtaacgta gtaagtagct cgctgtagta ccccatattg gtttgtggca acacccactg
      661 ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact tactggctac
      721 cagcacaaac tcggactcga acagcggctc aacatgtaaa tcctgaagct tcatttctgc
      781 gcttaacgta ccaatcgcaa aatccagtcg accgtcgcgg attgccggta agaacgaaga
      841 cagttgcgct tcatacatag aaacctgcgc tttcgggaac acctctttga acttgttgat
      901 catcccggac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga cttccaccac
      961 cgcctcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac gggtaatgga
     1021 ttcagaacgg gagagtaaca attgaccggc aggtgttagt gttacaccgg tatttttccg
     1081 caccactaat tccacgccaa aataatcttc aatatcgtta atgattttac tgacggccgg
     1141 ttgagttaac cctaattctt ttgcagccga gccgatagag ccacttctaa tgacttcctg
     1201 aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga cctacgttaa
     1261 ttacctcagt gacggcatga agtgtatcaa aatgaaatga acaggatatg tgcgaccact
     1321 cacaaattaa ctttcaatac tttccatggt atcgttattc caattaaata tctttacata
     1381 tcaataagtt aaagtataaa aatcagcata aaccctgatt tttgtcaaaa aaaatgacgg
     1441 ggataaccat attttatggc gataacatca ttcgttatgt ggttaataca aaaaaggggc
     1501 tgagaccata tttcaagcaa ttaccggata attaccaggc aaaatttgag ttaaatttaa
     1561 tgagcaagtt ctcaaatttt attataaata aaccattttc agcgataaat aatgcggcac
     1621 gtcacatttt ttcacgttat ttgttgaaga acaaacattt attttatcaa tattttaaaa
     1681 tttcgaatac aggtattgat catctcgaac aattgattaa cgtcaacttt ttctcttctg
     1741 acaggatgtc attttgtgaa tgcaatcgtt ttccataaat tcctctcccc tcataggcga
     1801 cgaatagcat tttgtgttga ggatcacaaa acgaataatt gctggtcgcc gcgataaggt
     1861 cagacaaaga caacaaggga aattttcaca gagcttttga tcggcgtagg ccacagaatg
     1921 ttgcttcgtt tactgtaacg ccgggtaaat gagcgttttt tgatagtgcg aaagaaccct
     1981 gcgaggaaaa taagcatcta ttattgtttg tcatgatgac aaggcaggca tcaatgaaag
     2041 gcttcccaat tgcacatatt tttcaccctt caatcccgcc aatgcacgca gtggttaaca
     2101 atcacaacag aaatattgat tattggacgg taaaaagaaa gtttgcagaa attgtctcca
     2161 ccaatgacgt taataaaata tacagtataa gtaatgaact gcggagagta ttatctgcaa
     2221 ttactgcatt gaatttctat caaggcgatg ttccttctgt catgatccga atccaaccgg
     2281 aaaatatgag tccattcatc atagatattt ctacaggaga acatgacgat tatatcatac
     2341 aaacattaga tgtaggcact tttgcacctt ttggcgaaca atgtacttgc tcagccgtca
     2401 ataaaaaaga gctggagtgt attaaggaga cgatttctaa gtattgtgca aaattcaccc
     2461 gaaaagaagc catcttaacc ccccctacgt actttaataa aacctccatt ccttcagatt
     2521 gttggcaaat tctctttttt tcacccgatc attttaataa tgatttttat tg
//
LOCUS       DAOTRK010000283         2568 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.286, whole genome shotgun sequence.
ACCESSION   DAOTRK010000283 DAOTRK010000000
VERSION     DAOTRK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.286"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(11..1201)
                     /gene="aspC"
                     /locus_tag="QIE41_004717"
     CDS             complement(11..1201)
                     /gene="aspC"
                     /locus_tag="QIE41_004717"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDS3533486.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1386..2459)
                     /gene="ompF"
                     /locus_tag="QIE41_004718"
     CDS             complement(1386..2459)
                     /gene="ompF"
                     /locus_tag="QIE41_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDS3533487.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          631 a          646 c          623 g          668 t
ORIGIN      
        1 tttttaacgc ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg
       61 tcatcccggc tacgttcaca cgaccagaag caacagcata cacgccaaac tcttcgcgca
      121 gacgcagcac ttgttctttt gtcagaccac tgaaggagaa catgccgttc tgtttgatga
      181 taaagctgaa gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca
      241 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat
      301 cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag
      361 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcagcagcaa
      421 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
      481 caacaatcag ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca
      541 gaccacgggc aaacccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg
      601 aaagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
      661 agcagccatg gaacagcact acgtcgccag cctgagcttc gttcaggctg ttaatcagtg
      721 catcgaagtc aagggtgtga ttttccgcat cataataagc gtattcacga acttccagac
      781 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacacacgct
      841 taacgctggt attttttgcc aggaaatcgg cagccacgcg tagtgcgcca gtgccacccg
      901 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
      961 gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg
     1021 tttcattttc gagcaaatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
     1081 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
     1141 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
     1201 tgacgaggtt ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta
     1261 caaaaatcca actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
     1321 tggaaggcat aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggtg
     1381 tgctattaga actggtaaac gatacccact gcaacggtgt cgtcagaacc tacgcccagt
     1441 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
     1501 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
     1561 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
     1621 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgccttc aattggcgta
     1681 gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc atacttcaga
     1741 ccagttgccc actgttcagc ttttttacct tgaccgcgga attcggcttc ctgggcgtcg
     1801 gtacggtctg ctgcaccgta agcacctacg ataccaaagc cttcgaattc gtagctgata
     1861 gagccaccga cgccgtcgcc gttggaacgc tcaggaatac cagcacgttc gtttttgccc
     1921 aggtactgaa cggcaaagtt caggccgtca accagaccga agaagttgga gttacgatag
     1981 gtagcaacgc cgcctacacg accaacgaag aagtcatcgc tgtatgccgt gtcaccgcca
     2041 aactcaggca gcatatcggt gtaacccagt gcgtcgtaaa ccacaccgta gttacgaccg
     2101 tagtcgatag aacccgcatc agcgaatttc agacctgcga atgccagacg agttttgtta
     2161 cctttctggg catcagcgcc ttcagagttg ttaccctgga agttatattc ccactgaccg
     2221 tagccggtca gatcggaatt gatttgtgtt tcccctttaa aaccaagacg ggcataagtt
     2281 ttgtcgccgt ttccgccgta actgtttaca ccattgtctt tagaaaaata atgcagaccg
     2341 acagctttac cgtacagatc tactttgttg ccatctttgt tatagatttc tgcagcgttt
     2401 gcagtacctg ctactaacag agcagggacg atcactgcca gaatattgcg cttcatcatt
     2461 atttattacc ctcatggttt tttctatgac acctgccact gccgtcaata agttctgtca
     2521 ataaaaattt acggaactat tgatgagagt ttggtgtctt tatgtgtc
//
LOCUS       DAOTRK010000284         2566 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.287, whole genome shotgun sequence.
ACCESSION   DAOTRK010000284 DAOTRK010000000
VERSION     DAOTRK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.287"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1..2466
                     /locus_tag="QIE41_004719"
                     /pseudo
     CDS             1..2466
                     /locus_tag="QIE41_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          647 a          652 c          795 g          472 t
ORIGIN      
        1 cggcgtctgg gcatagagta cgaccaccac aaccggccgg aaacactcac cgacgtgatg
       61 ggccgcgaac accacaccga atacagtggt cacgacctgc cggtgaagat gcgcggcccc
      121 ggcggtcagt cagtgcgctt acagtggcag cagcaccata aactgagtgg ccttgagcgc
      181 actggcaccg gcgcggaagg attccgctac gaccgccacg gcaacctgct ggcctagacg
      241 gacggtaacg gtgttgtctg gacaatggaa tacggcccgt ttgatttgcc ggtggcgcga
      301 acggacggtg aaggccaccg ctggcagtac cgctacgata aagacacgct gcaactgaca
      361 gaagtcatta acccgcaggg tgagtcatat ctttatgttc tggataactg cggccgggtg
      421 acggaagagc atgactgggg cggcgtggtc tggcgttacc gctatgacgc cgatggcctg
      481 tgtaccgcca gggtcaacgg cctggaggaa accatcctct acagccggga cgccgcaggc
      541 cgcctggcag aagtcatcac gccggaaggc aaaacgcagt atgcctatga caaatccggc
      601 aggctgacgg gtatcttcag cccggacggc acatcacagc gcaccggcta tgacgaacgc
      661 gggcgggtga atgtcaccac tcagggccga cgggccattg aataccacta ccccgacgaa
      721 cacaccgtca tccgctgtat cctgccaccg gaagatgaac gcgacagaca ccccggtgaa
      781 tccctgctga aaaccacgta ccgctacaac gccgccggag aactgacgga ggtcattctg
      841 ccgggggatg agacgctgac gttcagccgt gatgaggcag gacgtgaagt gctccggcac
      901 agtaaccggg gttttgcctg tgaacagggc tggaatgcag ccggtcagcc tgtcagtcag
      961 cgcgccggac ttttcccggc agaagccaca tggggcgggc tgctcccctc acgggtacgg
     1021 gagtaccgtt acgacagcgc gggtaatgtg tcaggcgtca ccagccggga agattacgga
     1081 cgggaaacac ggcgggaata ccggctggac cggaacggcc aggtcacggc ggtgacagcc
     1141 tcaggcaccg ggctgggcta tggcgaaggc gatgagtcct atggctatga cagctgcggc
     1201 tacctgaagg cgcagtctgc gggctggcac cggataagtg aagagactga ccagtatgcc
     1261 ggaggccacc ggctgaaaca ggccggaaac acgcagtatg actatgacgc cgcaggccgg
     1321 atggtcagcc gcaccagaca ccgtgacggc tatcgcccgg aaacagagcg gttccggtgg
     1381 gacagccgtg accagctgac cgggtattgc agcgcacagg gtgagcagtg ggaataccgc
     1441 cacgacgcca gcggcagacg aacagagaaa cgctgcgacc ggaagaaaat ccgcttcacg
     1501 tatctgtggg acggcgacag tattgcggaa atccgggaat accgcgatga taaactgtac
     1561 agcgtacggc acctggtgtt taacggcttt gagctgataa gccagcagtt cagccgggta
     1621 cggcaggcgc atccgtcggt ggccccgcag tgggtgacac ggacgaatca tgcggtgagc
     1681 gacctgacgg gccgtccgct gatgctcttt aacagtgaag gtaaaaccgt ctggcgaccg
     1741 ggacagacca gcctgtgggg gctggcactc agcctgcccg cagacaccgg ctacccggac
     1801 ccgcgcgggg aactggaccc ggaagccgac cccggcctgc tgtatgcggg acagtggcag
     1861 gatgcagaat ccgggctgtg ctataaccgg ttccggtatt acgagccgga aaccgggatg
     1921 tacctggtga gtgatccgct ggggttgctg gggggtgagc agacttaccg gtatgtgcca
     1981 aatccgtgtg ggtgggttga tccgctggga ttggctgcaa gttctaaaat cagcagtttg
     2041 atggactata ttggcgatgg tcgtcgtgtt agtgggcata cgggtttcct ggatggggtt
     2101 cgtttatcac gtagtcaaat aaacaatatt gctaaagaaa tggagaagct aggaattaaa
     2161 gtaataagga aagcagataa atatttgcca ccaaatgcta gggcagcttt tgattatggc
     2221 cttcgcaata tttatcttag gaaaaatgct accttatatg aggtgtatca tgaagtgatt
     2281 catgctaagc aatttgcgaa aattggacga gaagcatacg aagcactagg acgtttatct
     2341 agggaggaac acgttctaaa tgaaatatta aaaagtaaaa atttattcaa tgaggcggaa
     2401 atagctcatg ccataaaata tgttgaggga ttgagagaaa aattcatgat gggactaata
     2461 aattgataaa acataacaaa ataactatcg aaatggctct tgacttggct cggcgtgagt
     2521 tagagttaag ggaaattcct tatattaaaa atagtctaca tgccaa
//
LOCUS       DAOTRK010000285         2559 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.288, whole genome shotgun sequence.
ACCESSION   DAOTRK010000285 DAOTRK010000000
VERSION     DAOTRK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.288"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1085)
                     /locus_tag="QIE41_004720"
     CDS             complement(<1..1085)
                     /locus_tag="QIE41_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3533488.1"
                     /translation="MDNLIGTPPNYVVPHKYIDTEQMEYLCHLNRFSKSPDDFLITGQ
                     REDLFTYKYILDGSFSNLQHLFPKGQLQKIQQRLSSLMYKNMFHCFHVFLLKLCSIES
                     IPLPNADYAFFDDEMPFTLTDEQIENISFLNAYHKEKKGNNDIVTFDFMSADHHHNFS
                     TTIALTNDSFHISSINNHNAQVIFDENIHLHPYELPESSQWCYQLIKNMISLHCRYNN
                     NFKIKKTCCFDFKNKSFCNARLQYITFIACLFSHANMKYSTFHDVNLDMCEIKNCNFD
                     NSEMNFISCVGTNFSGSTFNNVKTTTAQLIKTPTKWTNNTLKYWFSSCNKRNIIFTFN
                     TISDRNMKLKGINDILLSLVDQKVNIY"
     gene            1394..>2559
                     /gene="espY4"
                     /locus_tag="QIE41_004721"
     CDS             1394..>2559
                     /gene="espY4"
                     /locus_tag="QIE41_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY4"
                     /protein_id="HDS3533489.1"
                     /translation="MVAKLKPDVLFNTNPFLEAMNKERLNHKGYCDKIALSINDKKYN
                     VNSKDIENILDGKGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEFVTKVDNKAEVFDK
                     IKSLAKKEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGATSNDNEKKQVSSERLTLDN
                     YIDDLHFDNSPLKRFMFDDYAVKLTTLIKNKIPIINTTINLSSLSKDVLNSLKYCSFK
                     NVVFSGVINSPNLEGPVFENCYFEDCKFNNIQLYETNDDTVGSGNKKPIKGMFKGCFI
                     SKCKIENYRCETSKIYTVTQPDSIQEKLGSYLFMQSFVQDCIIQGGCCPGSSILLSHF
                     YNCNIIGLDAHGMDFLANSFNKSNYDEIRGPDTGTVFYNCNLKHIKINDGLREDG"
BASE COUNT          879 a          355 c          444 g          881 t
ORIGIN      
        1 ctataaatat tgactttttg gtcaaccaac gacagtaata tatcattgat accttttaat
       61 ttcatatttc tatcagatat tgtattaaaa gtgaagatga tattacgttt attacaactg
      121 gagaaccaat attttagagt attattcgtc cactttgttg gtgtcttaat tagttgcgct
      181 gttgttgttt ttacattgtt aaatgtagaa ccgctaaaat ttgtcccgac gcacgaaatg
      241 aaattcatct ctgaattgtc aaaattgcaa ttctttatct cacacatatc aaggttaaca
      301 tcatgaaatg tcgaatattt catgtttgca tgtgaaaata aacatgcaat aaacgttata
      361 tattgcaacc tggcattaca aaaagattta tttttaaaat caaaacaaca ggtttttttt
      421 attttgaaat tgttattata tctgcaatgt aaagaaatca tgttttttat taactgataa
      481 caccattgtg atgattctgg taattcatag ggatgtaaat ggatgttttc atcgaatata
      541 acctgtgcgt tatggttgtt tattgaagat atatggaagc tatcatttgt taaagctatg
      601 gttgtgctaa aattatggtg gtgatcagcg ctcatgaaat caaatgtaac aatgtcgttg
      661 tttcctttct tttctttgtg atatgcgttt aaaaaagaaa tattttcgat ctgttcatca
      721 gttaatgtaa atggcatttc gtcatcaaag aaagcgtaat cggcatttgg gagaggaata
      781 ctctcaattg agcatagttt taataaaaaa acatgaaaac aatgaaacat gtttttatac
      841 ataagagaac ttaatcgctg ttgtatcttc tgtagttgtc ctttgggaaa taaatgctgt
      901 aaatttgaaa atgagccatc taaaatatat ttatatgtaa aaaggtcctc tctctgacca
      961 gttatcaaaa aatcgtcagg tgatttagaa aaacgattga gatgacataa atattccatt
     1021 tgctccgtgt ctatatactt atggggaact acgtagtttg gtggtgtgcc aataagatta
     1081 tccataaatc accttgataa aagttaataa aatctcttta ttttaacctc gacaataaaa
     1141 gcggctataa aaaatcgctc attttctgat gctgcggtta atccgtcgaa tgtgccgtta
     1201 acccactaaa aattgcaaat ccagggtaaa caccctgggt taaaatggaa ataatgagct
     1261 attttttaca gtattcaccg gtaatataag taatacttgt tgctaaccta actagcagta
     1321 cagttaatta acaacataga agttattgtt tttcaaacaa ctcaacaact caatattgca
     1381 cgccggagga tttatggtcg ctaaattaaa accagatgtt ctttttaata ctaatccttt
     1441 tttagaagca atgaataagg agagacttaa tcataaagga tattgtgata aaattgcttt
     1501 aagtattaat gataagaaat ataatgtaaa ctctaaggat attgaaaata ttcttgatgg
     1561 caaaggggat ttatttaaaa aacgtacact gtgggagttt gttcgcgatc tttttcccgg
     1621 ctctcatatt aaagaagtca aaggcttaat ttacgaattt gtcacgaaag ttgataacaa
     1681 agccgaggtg ttcgataaga ttaaatctct ggcaaaaaaa gaacaacaat ggcgatttag
     1741 tactaagaca gactttacca caaatgaaaa taatgaagtt attgtgagta gatcctttaa
     1801 tctctacacg ggggctactt cgaatgataa cgaaaaaaaa caggtttctt cagagagatt
     1861 aacgcttgat aattatattg atgatttaca ttttgataat tctcccttga agagatttat
     1921 gtttgatgat tatgctgtaa aacttacgac actgattaaa aataaaattc ctattattaa
     1981 tacaacaatc aatttatcat cgttgtctaa ggatgtttta aattcattaa aatattgtag
     2041 ttttaaaaat gtagtttttt caggagtgat aaacagccct aaccttgaag gtccggtttt
     2101 tgaaaattgt tatttcgagg attgtaaatt caacaacata cagctttatg agactaatga
     2161 tgatactgtg gggtcaggaa ataaaaaacc aataaaaggt atgtttaaag gttgttttat
     2221 ttctaaatgc aaaattgaaa attatagatg tgagacgtct aaaatctaca ccgttacaca
     2281 acctgatagt attcaagaaa aattaggttc ttatctcttt atgcagtctt tcgttcagga
     2341 ttgtataatc cagggaggat gttgtcctgg ctcgagtata cttctgagtc atttttataa
     2401 ctgtaatata atagggcttg atgctcatgg aatggatttt ttggcaaatt cgttcaacaa
     2461 gtctaattat gatgaaatca ggggtccgga tacggggact gtgttttata attgtaattt
     2521 gaagcatatt aaaataaacg atggtcttcg tgaagatgg
//
LOCUS       DAOTRK010000286         2539 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.289, whole genome shotgun sequence.
ACCESSION   DAOTRK010000286 DAOTRK010000000
VERSION     DAOTRK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.289"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            335..826
                     /locus_tag="QIE41_004722"
     CDS             335..826
                     /locus_tag="QIE41_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS3533490.1"
                     /translation="MSTDTLEIFNNSDEWASQLKHALSQGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDESVKSKAQAYIELLTDNSE
                     DNG"
     gene            1161..2537
                     /gene="ygeH"
                     /locus_tag="QIE41_004723"
     CDS             1161..2537
                     /gene="ygeH"
                     /locus_tag="QIE41_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HDS3533491.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQIYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQNISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFKPDYFVTGRINQNNAVKTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHNPERSVRLAKPDQGYKNHYLSDELLAGKKELYEFTPESIYR
                     AMAIFDGLQNKSDIQTLKIECYCLLAECHMSLALHGKYELELAAQKALELLHYVSDIT
                     NVDGKILAIMGLITGLSGQAKVSHILFEQAKIHTTDIASLYYYRALVNFHNERIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKSNMKLYYKETSSESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          951 a          419 c          417 g          752 t
ORIGIN      
        1 ataataaaga aaaaaaacta gacgacttac tacccttcgc aaaaatcccc tttttaattt
       61 tcatcgaaaa aagggaaatt gataatatga atcaaggtca tgcatattaa ggcatttatg
      121 aaatgtcgta aataagataa ctaataaggt gaatattagc aaaacaacta atgacattat
      181 tggtttatag tggtcagtat ttttactaat aataaaccaa tataccctta agcaatgcaa
      241 gcttaaaact cataattcaa aaaagataaa tgcaaaaaat aatgagtcaa ttaataataa
      301 tttagcatca cgaaacacat cacagaggaa tattatgagc acagatacac ttgaaatatt
      361 caataacagt gatgaatggg caagtcaact aaaacacgca ttatcacaag gagaaaatct
      421 ggcattacta catggattga ctcctgatat ccttgataga atatatgcat atgcattcga
      481 ctaccatgaa aaaggtaata taacggacgc agaaatttat tataaatttc tgtgcattta
      541 tgcgttcgaa aatcatgaat atctaaaagg ttttgcatca gtatgccagt caaaaaagaa
      601 atatcaacaa gcatatgacc tttacaaact aagttacaat tactccccgt atgatgacta
      661 ttcagttatt tatcgtatgg gtcaatgtca aattggggct aaaaatatcg ataacgcaat
      721 gcaatgtttc tatcatatta ttaacaattg tgaggatgaa agtgttaaga gcaaagcgca
      781 ggcatatatt gaactcttaa ccgataattc agaagataat ggctaagcat tacaagcatt
      841 tggtaaaaat tgctggttat agtgcagtta accactatga ccagcttctc ttatgttttc
      901 ttaaatcaac atcttaaaac agttaagaac acactataaa ttactatttt tcaacgcgtt
      961 agagaacaat ctattttatc aaaattgaat aaggctattt cagattacat cactgaattc
     1021 ctaagataaa ttagtgaaca caatcataga tatgtcgaat gtaaactcct gtagcattaa
     1081 tgacacataa atatgaatag ccacaatttc tattgcttat gcaggatgca agaaaccaat
     1141 tttttcatag aggataacta atggacttag aaaataaatt ctcatatcat tttctcgagg
     1201 gattaacgct cacggaagat ggaattctta ctcaaggaaa tgagcagatt tatattccac
     1261 cgaaagagtt aggagtattg atcgtattac ttgaatccgc tggtcatgtt gtcctgaaag
     1321 atatgatcat cgaatcagta tggaaaaata ttattgtaag tgacgagtcc ctgacaagat
     1381 gtatctattc tttgcgctgc atttttgaaa aaattggcta tgatcgttgc atagaaacaa
     1441 tctaccggaa aggttatcgt ttcagtggac aggttttcaa aactaaaata aatgaagata
     1501 acacttcaga ctattccata gctatatttc ctttcaccac ttcattgaat actctggatc
     1561 cattaatact taatcaggaa ttagtgcaaa acatttcgaa taaaaaaatc gatggccttt
     1621 atacctatcc gatggctgcg acaaattttt gtaatgatca catatcgcaa aattcatttt
     1681 tgagaagatt caagccagat tatttcgtta caggaagaat aaaccagaat aacgcggtga
     1741 aaactttata cattgaactg atcgacgcta aaaatctttt cctcatcgcc agtaatcatc
     1801 ttcctgttga tgaactacat aacacatcac aatttattat agataatatc cttcaaacgg
     1861 tacataatcc agagcgttct gtaagattag ctaaaccgga ccaaggttat aagaatcatt
     1921 atttatcaga tgaactgttg gctggtaaga aagaacttta cgagtttact cctgaaagca
     1981 tttacagggc tatggctata tttgatggat tacaaaataa aagtgatata cagacgctaa
     2041 aaatagaatg ttattgtctg ctagcggaat gccatatgtc tttggcactt catggaaagt
     2101 atgaacttga acttgctgct caaaaagcat tagagctttt acattatgta tcagacataa
     2161 ccaatgtcga tggaaaaatt ttagctatta tgggactgat aactggtctg tctggacaag
     2221 caaaagtatc tcatatctta tttgaacagg ctaagataca cacaactgat atagcctctc
     2281 tctactacta tagagcactt gtcaattttc ataatgaaag aattgaagag gcaaggattt
     2341 gtatagacaa atcactacaa ctcgaaccca gaagacgaaa agcagttgtg ataaaagagt
     2401 gtgtagatat gtatgtgcct aacccgctca aaagcaacat gaaactctac tataaagaaa
     2461 cttcgagtga aagccatcga gttataattg acaacatttt gaaactaaag caactgacga
     2521 gaatttgtat gcgataaat
//
LOCUS       DAOTRK010000287         2405 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.290, whole genome shotgun sequence.
ACCESSION   DAOTRK010000287 DAOTRK010000000
VERSION     DAOTRK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.290"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(593..1195)
                     /locus_tag="QIE41_004724"
     CDS             complement(593..1195)
                     /locus_tag="QIE41_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533492.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(1289..1495)
                     /locus_tag="QIE41_004725"
     CDS             complement(1289..1495)
                     /locus_tag="QIE41_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDS3533493.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          581 a          577 c          663 g          584 t
ORIGIN      
        1 gtctgactcc aattgaatat cggaatcaga cctatatgcc tcgtgtttaa ctgtccaact
       61 ttttggggtc agtacagaat gaggcttgcc atcagccgaa acatcagtat tccacgccat
      121 acagacggcg cctgaccatg tgctgatgca ggtaaaatgt ttcactacaa ctgttcgggt
      181 tgctatgcaa cgaaacagcg attctcacga tctttaactg caacgttttt ccgcctgaaa
      241 gctgactgcc tcctgctgag gtgggaggct atgattcagt gcgtttatag tctccggggt
      301 atgcatgtac cggagcatac catttctggt ggataacaag ccggcacatt cagactgcag
      361 tgaagatgga ggcacctggt tgtgaagaac gtttctgcac gtggctgctt cgcatcatca
      421 atggtattca tacccgctca gaaatgaccg ggtgctgaag ccctccgtta tcgcgcagta
      481 taatgacgta tatgatctgt ggacgagtga gcggcgtgcc tgatgtacgc cgctattact
      541 gtattttacc acagggctgg ttttctgctg ccagccctgt ggttctcaga catcaactgt
      601 gagcggcatc aaccgatgta cggtatgcca taagcacgta ctgatccatc agatttgtca
      661 gtggttcact ctgaccctga cgtgcaatga tccattgctc cagatctttt tctgatttgt
      721 gtttcaggtt tttcgtccag tcgtcaaggc tgtttccggt cattaagtca agccagcaga
      781 gccagaccag tttgagacgt aactccgtgc cgccaaactg acggaagagg ctgatcagtt
      841 tgtttgccag agaatgttgc ttcaggcgct cacattcaga cagcaggatc ccttccagca
      901 tcggatgatg aaccagcagg tgttcgctgg tgcataaatt caccaccaga tcatcatcca
      961 gggacgggga gctccgtttg tccggaccat aaatcagccg gtgcagactc tggggaacga
     1021 tggcaatctc cggtttttcg ccattgaatg acacccaccc gggcgtcaga ttatggaaat
     1081 agtagatatt cggatgacac acgccccatg atacggcgtg ccgggtgaag ccggtcagcc
     1141 atttctggta gttttcatgg agcagctcgt aacgggtctg atactgttgt ttcattttca
     1201 tattcctcac gggggataac aggcagcaga gcgccgccgg caggccggag attgcgtctg
     1261 ctgtgggcga agggttagtg gttaaaaatt acggacggga ctgtgcaata cgcgcctgca
     1321 gccaggcttc cacttcactt ttcagccagc gggagctgcg ccccattttg atgggcgcag
     1381 gaaagccccc atctttgatg agtttgtcaa accacttatc ggttaggccg gtcagttgag
     1441 tgataaacgc catgtcgacc atctggtcat ccatcagcga aactggggta gccatgagaa
     1501 tgttcctccg gatgttgtta aaccagccgg tgtggtcatg aaaacagcgc gccaccttca
     1561 ccggcataac aaccggggag aaacgtaaaa aataatcggg aatgtggctg tccggagata
     1621 aacgggggga ttgcgatggc agagccgtag cggaatcccg gggggggggg ggtgttgcga
     1681 aaagatgctt ccgcacgcac gcgcgataaa gaggggatag ttttcgtgag gacagggaga
     1741 gaagcaggcg ttctgataat tgcagagttg ttttcggagg tggttacagt gagtcagccc
     1801 gttcctggcg agcaggagac tgatatcacc gatgggataa ggttgtctga ttggtattat
     1861 aaatataaga agtcatcaga gagtaacgta aaggatttgc cagaataacc taagagagta
     1921 aatctctccg gtaacagagc gctgtgaagc attctgtatc agagtgcaga tacatgaacg
     1981 ctgatgcgta aaatcagcag tgacacgcca gctcccccta tgcccattcc tgtataagac
     2041 atgaactgag tgtattaatg cttctgccct gagactgagc cggtgagtga ggggctgaga
     2101 aatccctctt gacacagctc taaccagaaa tacttccccg gttgttgcgg tgcgtccgga
     2161 tccccgataa cggaacacta aggctgtatt gcgggacacc gggtaagtag cagcccgtaa
     2221 ggcgttctgt caacgccaga aaaaatgatt tcccacacgc gttatagact cactcctgtg
     2281 gacctggaag cccgtaactc tgcggttgcc cactgactga cggaactgac cgcaaatttt
     2341 acgagtgcgt gctgggcttc agtctggtca cctgagcgtt cggtaacgga ggcgcgggcg
     2401 gtaat
//
LOCUS       DAOTRK010000288         2392 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.291, whole genome shotgun sequence.
ACCESSION   DAOTRK010000288 DAOTRK010000000
VERSION     DAOTRK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.291"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            88..492
                     /gene="ybgC"
                     /locus_tag="QIE41_004726"
     CDS             88..492
                     /gene="ybgC"
                     /locus_tag="QIE41_004726"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDS3533494.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            489..1181
                     /gene="tolQ"
                     /locus_tag="QIE41_004727"
     CDS             489..1181
                     /gene="tolQ"
                     /locus_tag="QIE41_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDS3533495.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            1185..1613
                     /gene="tolR"
                     /locus_tag="QIE41_004728"
     CDS             1185..1613
                     /gene="tolR"
                     /locus_tag="QIE41_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDS3533496.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            1678..>2392
                     /gene="tolA"
                     /locus_tag="QIE41_004729"
     CDS             1678..>2392
                     /gene="tolA"
                     /locus_tag="QIE41_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDS3533497.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAVADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAAEKKA"
BASE COUNT          652 a          542 c          680 g          518 t
ORIGIN      
        1 acttgccctc attcccaaac ctcaatcgcg cgcgtatagt agcagcgttt aaaagctcta
       61 acttttgttg cattaccggg atgtaaagtg aatacaacgc tgtttcgatg gccggttcgc
      121 gtctactatg aagataccga tgccggtggt gtggtgtacc acgccagtta cgtcgctttt
      181 tatgaaagag cacgcacaga gatgctgcgt catcatcact tcagtcaaca ggcgctgatg
      241 gctgaacgcg ttgcctttgt ggtacgtaaa atgacggtgg aatattacgc acctgcgcgg
      301 ctcgacgata tgctcgaaat acagactgaa ataacatcaa tgcgtggcac ctctttggtt
      361 ttcacgcaac gtattgtcaa cgccgagaat actttgctga atgaagcaga ggttctggtt
      421 gtttgcgttg acccactcaa aatgaagcct cgtgcgcttc ccaagtctat tgtcgcggag
      481 tttaagcagt gactgacatg aatatccttg atttgttcct gaaggctagc cttctggtta
      541 aacttatcat gttgattttg attggttttt caatcgcatc ctgggccatt attatccagc
      601 ggacccgtat tcttaacgca gcggcgcgtg aagccgaagc gtttgaagat aaattctggt
      661 ctggaatcga actctctcgc ctctatcaag agagtcaggg gaaacgggat aatctgactg
      721 gttcggaaca aatcttttac agcgggttca aagagtttgt gcgcctgcat cgtgccaata
      781 gccatgcgcc ggaagccgta gtggaagggg cgtcgcgtgc tatgcgtatc tccatgaacc
      841 gtgaacttga aaatctggaa acgcacattc cgttcctcgg tacggttggc tccatcagcc
      901 cgtatattgg tctgtttggt acggtctggg ggatcatgca cgcctttatc gccctcgggg
      961 cggtaaaaca agcaacactg caaatggttg cgcccggtat cgcagaagcg ttgattgcga
     1021 cggcaattgg tctgttcgcc gcaattccgg cggtaatggc ttataaccgt ctcaaccagc
     1081 gcgtaaacaa actggaactg aattacgaca actttatgga agagtttacc gcgattctgc
     1141 accgccaggc gtttaccgtt agcgagagca acaaggggta agccatggcc agagcgcgtg
     1201 gacgaggtcg tcgcgatctc aagtccgaaa tcaacattgt accgttgctg gacgtactgc
     1261 tggtgctgtt gctgatcttt atggcgacag cgcccatcat cacccagagc gtggaggtcg
     1321 atctgccaga cgctactgaa tcacaggcgg tgagcagtaa cgataatccg ccagtgattg
     1381 ttgaagtgtc tggtattggt cagtacaccg tggtggttga gaaagatcgc ctggagcgtt
     1441 taccaccaga gcaggtggtg gcggaagtgt ccagccgttt caaggccaac ccgaaaacgg
     1501 tctttctgat cggtggcgca aaagatgtgc cttacgatga aataattaaa gcactgaact
     1561 tgttacatag tgcgggtgtg aaatcggttg gtttaatgac gcagcctatc taaacatctg
     1621 cgtttccctt gcttgaaaga gagcgggtaa caggcgaaca gtttttggaa accgagagtg
     1681 tcaaaggcaa ccgaacaaaa cgacaagctc aagcgggcga taattatttc agcagtgctg
     1741 catgtcatct tatttgcggc gctgatctgg agttcgttcg atgagaatat agaagcttca
     1801 gccggaggcg gcggtggttc atccatcgac gctgtcatgg ttgattcagg tgcggtagtt
     1861 gagcagtaca aacgcatgca aagccaggaa tcaagcgcga agcgttctga tgagcagcgc
     1921 aagatgaaag aacagcaggc tgctgaagaa ctgcgtgaga aacaagcggc tgaacaggaa
     1981 cgcctgaagc aacttgagaa agagcggtta gctgctcagg aacagaaaaa gcaggctgaa
     2041 gaagccgcaa aacaggccga gttaaagcag aagcaagcgg aagaagcggc agcgaaagcg
     2101 gcggcagatg ctaaagcgaa ggccgaagcg gatgctaaag ctgcggaaga agctgcgaag
     2161 aaagcggttg cagacgcgaa gaaaaaagca gaagcagaag ccgccaaagc cgcagccgaa
     2221 gcgcagaaaa aagccgaggc agccgcggcg gcactgaaga agaaagcgga agcggcagaa
     2281 gcagctgcag ctgaagcaag aaagaaagcg gcaactgaag ctgctgaaaa agccaaagca
     2341 gaagctgaga agaaagcggc tgctgaaaag gctgcagctg agaagaaagc gg
//
LOCUS       DAOTRK010000289         2357 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.292, whole genome shotgun sequence.
ACCESSION   DAOTRK010000289 DAOTRK010000000
VERSION     DAOTRK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.292"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(459..893)
                     /gene="uspF"
                     /locus_tag="QIE41_004730"
     CDS             complement(459..893)
                     /gene="uspF"
                     /locus_tag="QIE41_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDS3533498.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1034..2167)
                     /gene="ompN"
                     /locus_tag="QIE41_004731"
     CDS             complement(1034..2167)
                     /gene="ompN"
                     /locus_tag="QIE41_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDS3533499.1"
                     /translation="MKSKVLAFLIPTLLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          669 a          494 c          541 g          653 t
ORIGIN      
        1 tcctgctggc gacgcgttat tgaggaaaaa agtaaggcag ctagtaggct ttgaaatgac
       61 ttgatgagga aaattgttga tggcaaaaat ggggcaaagc gctgcaaaag gggcaaaaaa
      121 ggggcaaaaa aagagtgggt tatcgtagct tattgttgtc gctgatgata tttaacacat
      181 tgaaaaataa gtaaaatact tatgagtcag agagttgtga tttttgccct tacttgttca
      241 ggttgtattg ttctttctta ctaatttctt gattttgcga catttaaaag cgactcaatt
      301 cgttatatgg catcagaaga gtatgcgtca tgccggaacg cccagcataa gaaatctgat
      361 ataaaaactg tggcgtgtat ggtacggatt agaggggaaa atgtcagcac atttgcgaaa
      421 tgaatcaaaa agcccgcagc aatgtgcggg cgttagtgtc agcgcacaac cagcacggag
      481 cactctgcgt gacgcacgac agctgcggcg ttggaaccga gcagataagt ggtgatatcc
      541 ggtcgatggg aagcaatgat gatcatatga gcggggatct tcttcgccaa ttccagaatg
      601 cggtctttgg gcgagccttc ctcaacatgg acatgcactc tgtcggttgg cagtttaaat
      661 tttttaatga tctcttccag ttgcgatttg gcttccgctt tcaggtcatc cattgccggt
      721 aattctgcgg aatacgctaa acccagagag gcatagtagg gcagtgaagg tattaccgtc
      781 aggaaatgaa cctctgcatc atcaatcttt gcctctgcct caacgtggct aatcacgcgt
      841 tgagttaatt ctgaatcgga aatatcgata gggacaagaa tcgttctgtt cataaaacct
      901 cctgttttag tatccgcata aagtgtaacg ccagatgaca ctttttgtgt aatgacgaag
      961 ttcacatttt taatttagat caaaggagga agaataagca gaaaaagccc gccataacag
     1021 cgggcaggag gatttagaac tgataaacca gacctaaagc gacaatatca tcggtagaga
     1081 tgccgttggc agcgtagaag ctgtcatctt catccaacag gttgatttta tagtcaacgt
     1141 aggtggacat atttttgttg aaatagtaag tcgcgccaac atcggcgtat ttaaccagat
     1201 ctttatcatc aacacctgcc gggttgtctg caccaccggc agcgtgcagg tcacggcctt
     1261 tagacatcag gaaagagact gccggacgca gaccaaaatc aaactggtac tgtgcagtaa
     1321 cttcaaagtt ctgggttttg ttagcaacag cataatcgct gtcgccaaac ggggtcatat
     1381 tacgcgtttc tgaatacatg gttgccaggt aaatattgtt agcatcgtat tttagcccag
     1441 cagtccacgc gtctgcttta tcaccacccg ccgcagtatg gttaacctgg tcattggtgc
     1501 ggtcagaaga ggtgtatgcc gcaccagcgc taaagcccat gcctaaatca tatgttgtgg
     1561 aaagacccca gccgtcaccg ttttcatggc gaacatcacg tccgttgttg gtgccttcct
     1621 gaccattact ggcaccttcg ttgttacctt gatactgcac cgcgaagttc agaccattta
     1681 ccagaccgaa gaaatcagta ttacgataag tcgcgacgcc attggcacga ccagtcataa
     1741 agttgtctgc attggtataa gagtcaccgc caaattcagg cagcatatcg gtccagcctt
     1801 cgatgtcgta cattacgcca taattacgtc cgtaatcgaa agaaccgtaa tctgcaaatt
     1861 tcaatccggc aaatgccaga cgggtccatg actggttttt tgaagattca gtgttgtttg
     1921 cctgaatatt gtattcccat tgaccgtagc cagtaagttg atcgttaatt tgggtttcgc
     1981 ctttaaaacc cagacgcgca tagctctggt cgccatcttt cgctgaatta tcagaaaaat
     2041 aatgtagtcc atcaactttg ccatacagat ctaatttgtt gccgtcttta ttataaactt
     2101 cggctgcatg tgcagcacct gcggcgagca gggtaggaat taaaaatgcc agtactttgc
     2161 ttttcattga ataaatcctt tagttatttt atttgccttt tcatccccat tttggggagt
     2221 gaaaacatcc taaaggagaa gttccgttag ccgataaata tcatttgtta cgccttaagt
     2281 aaaaccttaa tcaaataatt ctacggaaat gatgataagt ttggtgaata tattattggc
     2341 gacatataca aaataaa
//
LOCUS       DAOTRK010000290         2308 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.293, whole genome shotgun sequence.
ACCESSION   DAOTRK010000290 DAOTRK010000000
VERSION     DAOTRK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.293"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            32..1291
                     /locus_tag="QIE41_004732"
     CDS             32..1291
                     /locus_tag="QIE41_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDS3533500.1"
                     /translation="MTNINTACVKNNASYQLNNALPNKETISSNFCERLAQWGNKSLN
                     NGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFTDN
                     ASLVNINLSFNKIKTITFPNESKLENIYIDHNNLESLDFKNQHSLVNLEAQNNNLTKI
                     NISDSYKLKFLNLDYNKLASLDLSRQESLIELSAHHNMINDIILHNHPIVEKITLNDN
                     HIAHLNAKATTKLEYLNLSNNNLLPTDDIDQLISSKHLWHVLVNGINNDPLAQMQYWT
                     AVRNIIDDTNEVTIDLSYNLAITNIDTSDEHLVEVSENSEGNHIKDNDSMSIRYRSKY
                     YSREYDLIEEETIFSDAELKAILPMHRMYGVGDYKSNSSSLPSHSGLKDPTGTPVCYY
                     IHNEDKPSLGFGPTSNNWLSQSFTTEL"
     gene            1354..>2308
                     /locus_tag="QIE41_004733"
     CDS             1354..>2308
                     /locus_tag="QIE41_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDS3533501.1"
                     /translation="MKFPSIFNKINPQSIQQHPEKNELNWMRELNKWKAERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLTTQPPGLQNFTSINLDENQLKHFDATTY
                     DRLINLSLNSNALESINFPQGRNVSITHISMNNNSLRNIDIDRLSSITYFSAAHNQLE
                     FVQLECCERLQYLNLSHNQLTDIVAGNKDELLLLDLSHNKLTSLHNTLFPNLNTLLIN
                     NNLLSEIKIFFSNFCNIQTLNAANNQLEKINLHFLTYLSSIKSLRLDNNKITRIDTKN
                     TSDIGTLFPIIKQSKNLNFLNVSGKNNCPTIQLMLFNLFSPA"
BASE COUNT          845 a          450 c          341 g          672 t
ORIGIN      
        1 atcttactat aatatttatt gatgagtgat tatgaccaat attaatacag cttgtgtaaa
       61 aaacaatgcc agttatcaat taaataacgc attacctaac aaagaaacca tctccagcaa
      121 tttttgtgaa cgactggcac aatggggtaa taagtcgctt aataatggtg aagaaagagc
      181 aattgccgta gagcgaatta aagaagctta caattcgaac atggcatctc ttgatttgtc
      241 atatcttgat ttgagtgagt taccgcctat cccctcgaca gttaacacgt taaatctgga
      301 aaacaattgt ctcacttgtc ttgactttac tgacaatgcc agcctcgtca atatcaacct
      361 cagctttaat aaaattaaaa cgataacctt tccaaatgaa tcaaaactgg aaaatattta
      421 tattgatcac aacaatctgg aaagtttgga ttttaaaaat cagcattcat tggttaacct
      481 ggaagcgcaa aataacaatc tgacaaaaat taatatttct gatagttata aactgaaatt
      541 tcttaatctt gattataaca agctagcatc gctggatctc tcccggcaag aatctctgat
      601 tgagttaagt gcccatcata atatgatcaa tgacattata ttacacaatc accccatagt
      661 ggagaaaatc actttaaacg acaaccatat tgcacattta aacgcgaaag ccactacaaa
      721 actggaatat ttaaacttaa gcaataacaa tttattgcca acagatgaca ttgatcaatt
      781 aatatcatca aaacatcttt ggcatgtatt agttaacggc atcaacaatg atccacttgc
      841 ccaaatgcag tactggactg cagtaagaaa tataattgat gacactaatg aagtgaccat
      901 tgatttatct tataacctgg caatcacaaa tatcgatacc agcgatgaac atcttgtaga
      961 agtaagcgag aattccgaag gaaatcatat aaaagacaat gactcaatgt ctattcgtta
     1021 tagatcaaaa tattattcca gagagtacga tttaatagaa gaagaaacaa tattttctga
     1081 cgcagaacta aaagccattc tgcctatgca tcgcatgtac ggggttggtg actataagtc
     1141 aaattcctct tctctaccct cacactcggg gctaaaggac ccaacgggta cacccgtctg
     1201 ttattatatt cataatgagg ataaaccttc cttaggtttt ggtccaacat ccaataattg
     1261 gttaagccaa tcctttacaa cagagttata aaacacatcc tgatttatca agggtaataa
     1321 atcattaaaa taaagcatta ctacggagca gttatgaaat tcccttcaat atttaataaa
     1381 ataaatccac aatccataca gcaacatcca gaaaaaaatg aacttaactg gatgcgcgaa
     1441 ttaaataaat ggaaagcaga acgcatactt acaggtgaaa tccatcgtcc ggaatgccga
     1501 aacgaagccg ctaaaaggat aaattgtgct tttttgtcga aacagaatga tattgattta
     1561 tcagggctta atttaactac ccaaccacca ggactgcaaa acttcacctc tatcaatctt
     1621 gatgagaacc aactcaaaca ttttgatgca accacctacg atagactcat caaccttagt
     1681 ctgaatagta atgctcttga gtcaataaat tttcctcaag gtagaaatgt aagcattaca
     1741 catatatcta tgaataataa ttctctcaga aatattgata tagataggct ttcatcaatt
     1801 acttatttta gtgcggcaca taatcaacta gagtttgtgc aattagaatg ttgcgaaagg
     1861 ctgcagtacc tgaatctcag ccacaatcaa ttaactgata ttgtggcagg aaataaagat
     1921 gaactcttac ttctggatct atcccataat aaactaacaa gtttacataa taccttattt
     1981 cccaacttga atacgttact tatcaacaac aacttgcttt ctgaaattaa aatattcttt
     2041 agcaacttct gcaatattca gacattaaac gccgcgaaca atcagttgga aaaaataaac
     2101 cttcatttcc tgacttatct ttcatctatc aaaagtttaa ggctggacaa taataaaata
     2161 acccgcattg acactaagaa tacgtccgat attggaactt tattccccat aataaaacag
     2221 agcaaaaact taaatttttt aaatgtttct gggaagaaca attgccctac tatccagctc
     2281 atgttattta atttgttttc cccagcac
//
LOCUS       DAOTRK010000291         2299 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.294, whole genome shotgun sequence.
ACCESSION   DAOTRK010000291 DAOTRK010000000
VERSION     DAOTRK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.294"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(108..317)
                     /gene="fumD"
                     /locus_tag="QIE41_004734"
     CDS             complement(108..317)
                     /gene="fumD"
                     /locus_tag="QIE41_004734"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDS3533502.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            874..2286
                     /gene="pykF"
                     /locus_tag="QIE41_004735"
     CDS             874..2286
                     /gene="pykF"
                     /locus_tag="QIE41_004735"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDS3533503.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDSRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          627 a          568 c          508 g          596 t
ORIGIN      
        1 acataccgaa attcgtataa tttcagcagt tgttatttag aatgattaac tggaccgcaa
       61 ctgaagcaag aatatcagga tgaagcttat cgcctcatcc tgaatggtca ttttaccagt
      121 gcgttaatga cggtttccat cgcctgcttt tccagctcac tgcgctgaat gcgtttattg
      181 gctaatgctg tacgtaatac gcctgcgata acaatatgct ttggttcctg ccccagatcg
      241 cgcatttcca gcacaacttt accgacaact ctgcacattt ctcggtacag ttcatcctct
      301 tttgtccgat ttcccattct ttatacctat ttatcatgcc aactatcagc atatatcaat
      361 ctaaccaatt agacaaaaat caaacaaaat cagacaaata acgcgataaa ttattttaac
      421 tgtagcaatt gagcgatgat gtatttatac accggatgaa ctttcactta tcttcacagt
      481 gacaaccccg gcaccagacg ttgcgcaaac agcgaagttt ttgcgtaacc ttttccctgg
      541 aacgttaaat ctttgataac aatttattgt cttacaagtt gtatattttt tgaaacgctg
      601 tttttgtttt ccttttggat taatttcagc gtataatgcg cgccaattga ctcttgaatg
      661 gtttcagcac tttggactgt agaactcaac gactcaaaaa caggcactca cgttgggctg
      721 agacacaagc acacattcct ctgcacgctt tttcgatgtc acctatcctt agagcgaggc
      781 accaccactt tcgtaatacc ggattcgctt tccggcagtg cgcccagaaa gcaagtttct
      841 cccatccttc tcaacttaaa gactaagact gtcatgaaaa agaccaaaat tgtttgcacc
      901 atcggaccga aaactgaatc tgaagagatg ttagctaaaa tgctggacgc tggcatgaac
      961 gttatgcgtc tgaacttctc tcatggtgac tatgcagaac acggtcagcg cattcagaat
     1021 ctgcgcaacg tgatgagcaa aactggtaaa accgccgcta tcctgcttga taccaaaggt
     1081 ccggaaatcc gcaccatgaa actggaaggc ggtaacgacg tttctctgaa agcgggtcag
     1141 acctttactt tcaccactga taaatctgtt atcggcaaca gcgaaatggt tgcggtaacg
     1201 tatgaaggtt tcactactga cctgtctgtt ggcaacaccg tactggttga cgatggtctg
     1261 atcggtatgg aagttactgc cattgaaggt aacaaagtta tctgtaaagt gctgaacaac
     1321 ggcgacctgg gtgaaaacaa aggtgtgaac ctgcctggcg tttccattgc cctgccagca
     1381 ctggctgaaa aagacaaaca ggacctgatc tttggttgcg aacaaggcgt agactttgtc
     1441 gctgcttcct ttattcgtaa gcgttctgac gttatcgaaa tccgtgagca cctgaaagcg
     1501 cacggcggcg aaaacatcca catcatctcc aaaatcgaaa accaggaagg cctcaacaac
     1561 ttcgacgaaa tcctcgaagc ctctgacggc atcatggttg cgcgtggcga cctgggtgta
     1621 gaaatcccgg tagaagaagt tatcttcgcc cagaagatga tgatcgaaaa atgtatccgt
     1681 gcacgtaaag tcgttatcac tgcgacccag atgctggatt ccatgatcaa aaacccacgc
     1741 ccgactcgcg cagaagccgg tgacgttgca aacgccatcc tcgacggtac tgacgcagtg
     1801 atgctgtctg gtgaatccgc aaaaggtaaa tacccgctgg aagcggtttc tatcatggcg
     1861 accatctgcg aacgtaccga ccgcgtgatg aacagccgtc tcgagttcaa caatgacagc
     1921 cgtaaactgc gcattaccga agcggtatgc cgtggtgccg ttgaaactgc tgaaaaactg
     1981 gatgccccgc tgatcgtggt tgctacccag ggcggtaaat ctgctcgcgc agtacgtaaa
     2041 tacttcccgg atgccaccat cctggcactg accaccaacg aaaaaacggc tcatcagttg
     2101 gtactgagca aaggcgttgt gccgcagctg gttaaagaga tcacttctac tgatgatttc
     2161 taccgtctgg gtaaagaact ggctctgcag agcggtctgg cacacaaagg tgacgtcgta
     2221 gttatggttt ctggtgcact ggtaccgagc ggcactacta acaccgcatc tgttcacgtc
     2281 ctgtaatatt gcttttgtg
//
LOCUS       DAOTRK010000292         2291 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.295, whole genome shotgun sequence.
ACCESSION   DAOTRK010000292 DAOTRK010000000
VERSION     DAOTRK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.295"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1096)
                     /locus_tag="QIE41_004736"
     CDS             complement(<1..1096)
                     /locus_tag="QIE41_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDS3533504.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTLLVSWFQSKM
                     QLATEAIKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALAIKLFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPL
                     ISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLW
                     LEDVENAGQPVPDELLPSEDKFKEGEDTKSE"
     gene            complement(1105..1872)
                     /gene="sctT"
                     /locus_tag="QIE41_004737"
     CDS             complement(1105..1872)
                     /gene="sctT"
                     /locus_tag="QIE41_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDS3533505.1"
                     /translation="MGEAMLYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIDK"
     gene            complement(1874..2134)
                     /locus_tag="QIE41_004738"
     CDS             complement(1874..2134)
                     /locus_tag="QIE41_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDS3533506.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(2144..>2291)
                     /locus_tag="QIE41_004739"
     CDS             complement(2144..>2291)
                     /locus_tag="QIE41_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDS3533507.1"
                     /translation="VLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDL
                     SINP"
BASE COUNT          759 a          456 c          384 g          692 t
ORIGIN      
        1 tctcgctttt tgtgtcttca ccttctttga atttatcttc tgaggggagc agttcatctg
       61 gaacaggttg tccagcattc tccacatctt caagccacag cagaagacgc aagatctcat
      121 ctatattttc gaagctaaca taatcgtagc gacgatgggt agcataaatt tttcgtgcta
      181 attttttatc tgtaataatt ggtattccga tttcctttgc atatttcctt acggctaatg
      241 ctacctcatt agtttctctc acagaaatca atggtatagg tgaaagatgt ggcttaaagt
      301 atatccctat ggcaatgtgc gtagggttgg caatcatcag acggctatta ctgacatcag
      361 atttcaattg ctctgacaga atttcttgat ggcgttctct gcgtttagac ttaatttctg
      421 gatttccttc ttgttccttg tattctcttt taacttcttg tttatccatt ttcatatctt
      481 tcataaataa aaaatactca gcgataaaat caaatattag aacaattatc atactgccga
      541 gacaatatag tatgagaagg aacagcatct ctccccaatc tgataataaa gaaatgatat
      601 ctccatcaag agttttaaaa agcagtgatt tatggttact ccaaaataat ttgatcgcca
      661 atgcaaaaaa aataatataa agtattgctt taacaaattc ttttacggtt tttaagccaa
      721 atatacgttt taagccattt actggattta atgaatcaaa cttaaactta atagcttcag
      781 ttgctaattg cattttactt tgaaaccatg aaacaagtag tgttgtaaac atacaaacca
      841 gcaaaaaagg aatgatcgtt ttcaaccaac ctatccccat agctttaaaa tagtcccaaa
      901 tatccggttt agcgttatga tcaaggatgt attcaagaat cgacataatg tgatgcacat
      961 caaatacata tccaagatat aaagtaccca caagcattat tacagagact gttaagtctc
     1021 tactttttag aatttgacct tttttagaag catcctggag tttcttttgt gttggctttt
     1081 cagttttatt tgccatgaaa cactctattt atcaatgtat aattgcaatt gttctgggaa
     1141 aaaggataac ggcataactc gttcagccag tattgggcca aaataaataa ttaatattaa
     1201 aaatgccagc gcacttttga cagtcagtga gatggcaaaa gcatttagtt gtgaagcata
     1261 acgtgccagc aatcccagga cagcttcacc gcccagcata accgcaatca cggggcttgc
     1321 gtaaacgata gtatgagtca ttatattatt aatatatgag aataaaggct ctaatttagg
     1381 aaaattaaaa tttcctgaag gccataagtt ataactttgc catagagtct ccagaataaa
     1441 gttcaatcca ccgttagtaa gataaactgc tgcagagaaa aggttaaata atcttgctaa
     1501 ttccgatgta tcgacaccag tagccggatc caaagtacta cttaacgttg caccacgttg
     1561 gttatcgata aagctgccta tagcaaggaa aatccaaaat ggggatgcta ataagcaagc
     1621 aataaacaac cctatgataa tttctctggc agcaaacaaa gcgatatcta tttcatttag
     1681 ggtagataat tcatagtggt aatgctgaac taatgcacat gataccacta ttattattgg
     1741 atgacggact actgttggta tattcccact ggcgaaaaat ggtaacaaat agaaagttgg
     1801 tgccaatcgg caaaaaccga gcgctgtagc agccagcaga ctatgcaact gatagagcat
     1861 cgcctccccc atgctatcct ctcgcgaatg ctaaattcag catttcgata ccaaacgaat
     1921 ataatttttc accataccat ccgctcatta aaaaaaagca aatacttacg cacaataatt
     1981 tgacgccaaa tggcaacgtc tgttcttgca actgtgttac cgtttgaaaa agccctacca
     2041 ataaaccaac aaatgtcgca actgcaatag gtcctgcaga cataactaaa atcagataca
     2101 gcgccctatt ccccgcaaaa acaatgtcgt ccatattttt tcctcaggga ttaatggata
     2161 ggtcaaaata ttgtaaaatg agtcctttcg ataacattgt ccaaccgtcc atcgcgacaa
     2221 acagaattaa ttttatcggt gtagatatgg ttaccggact catcatcatc atacctaacg
     2281 ttaacagaac g
//
LOCUS       DAOTRK010000293         2254 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.296, whole genome shotgun sequence.
ACCESSION   DAOTRK010000293 DAOTRK010000000
VERSION     DAOTRK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.296"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            102..>2254
                     /locus_tag="QIE41_004740"
     CDS             102..>2254
                     /locus_tag="QIE41_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HDS3533508.1"
                     /translation="MFSIKPGPRNLPIGNPTLLSWNITDGDLNSKLNTLEYLNCITNI
                     INSCGVYPQDLKHREIISTFHAEKVINDLLKNDYKISLFPDTTYRELNKAAQRSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPTGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRKNISEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIIDPVTKERDQIHPEDIHLTAKDFEASKDNISKDEWEN
                     LHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCPLDEQQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLQALASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNDDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKA"
BASE COUNT          790 a          435 c          407 g          622 t
ORIGIN      
        1 ggataaaagt aggacatctg tttgcaatta ctttcacaac aattaaacat ttttatgttt
       61 ccgtatacac catattactc taccattaga ggaactttat tatgttttct ataaaaccag
      121 gaccaagaaa tttacctatc ggcaacccca cattgttatc atggaacatt actgacgggg
      181 atctaaattc caaattaaat acattagaat atctaaactg tataacaaat attattaatt
      241 cttgtggagt ttatcctcag gatttaaaac acagagaaat tatatcaact tttcacgcag
      301 aaaaagttat taatgatctg ttaaaaaacg attataaaat ttcccttttt ccagatacaa
      361 cttaccgaga gttgaataaa gcagcacagc gtagcattac agcgccagac aggataggag
      421 aaggaaaaac atgggtttat caacgagata caatggttga aagaggtgat aacagcggtg
      481 ttcatcagta tggtccagct gaacatttta ctcacattat atctgacaaa ccgtccccaa
      541 aagataagta tgttgcatat gctattaaca ttcctgacta tgaactggca gctgatgtat
      601 ataatattaa cgtaacgtca cctaccggcc agcaagaaac atttaaaata ttaatcaatc
      661 cagaacatct acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat
      721 gtgaaatcat cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct
      781 atcaacaaat caggaaaaat atatctgagt ttgcacgttg tcattatggg tatatacaaa
      841 tccctcccgt aaccactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt
      901 actggtttca cgcttatcaa cccgaagatc tttgtactat ccataatcca atgggagatt
      961 tgcaggattt tatcgcattg gttaaagatg ccaaaaaatt tggtatcgat atcatccctg
     1021 attatacatt taactttatg ggaatcgggg gtagtggtaa aaatgatctg gattatccct
     1081 ctgccgatat acgagcgaag atcagtaaag atatagaaag tggtatccct ggctattggc
     1141 aaggtcaggt cttgattccc tttattatag atccagtaac aaaagaacgt gaccaaatcc
     1201 atccagaaga tatacatctc actgcaaaag acttcgaagc aagtaaagat aatatttcta
     1261 aggatgaatg ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa
     1321 caacacccaa aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa
     1381 aatatggcgt acgaggttta cgttatgatg cagcaaaaca ctcaaaacat gaacaaatag
     1441 agaggtcgat aaccccaccg cttaaaaatt ataatgagcg gttgcacaat acaaatttat
     1501 ttaatccaaa atatcaaaaa aaagctgtta tgaattacat ggaatacctg gtaacttgtc
     1561 cgttagatga acaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg
     1621 atttttcatt gctcatgaag acgataaaag ccttttcatt cggtggagat ctccaggccc
     1681 ttgcatcaaa accgggctcc acaatctcaa gcatcccatc agaaagacgg atattgatta
     1741 acatcaacca cgatttccct aacaatggta atcttttcaa tgacttttta tttaaccatc
     1801 agcaagatga acaattagca atggcatata tggccgctct cccgttcagc aggcctttag
     1861 tttactggga tggtcaggta ttaaaatcaa cgactgagat taaaaatgat gatgggtcta
     1921 ctcgtgtcgg cggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca
     1981 atgaattcca cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat
     2041 ttgcaactaa aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc
     2101 ctcatgatgg actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta
     2161 acaaattgca acctggaata tataaaaaca tggggagtaa tagcgttaac attattatta
     2221 ataatacccg aaaaattatc ccccctggta aagc
//
LOCUS       DAOTRK010000294         2214 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.297, whole genome shotgun sequence.
ACCESSION   DAOTRK010000294 DAOTRK010000000
VERSION     DAOTRK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.297"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            75..368
                     /gene="iss"
                     /locus_tag="QIE41_004741"
     CDS             75..368
                     /gene="iss"
                     /locus_tag="QIE41_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDS3533509.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(459..641)
                     /gene="rzoD"
                     /locus_tag="QIE41_004742"
     CDS             complement(459..641)
                     /gene="rzoD"
                     /locus_tag="QIE41_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDS3533510.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(858..1355)
                     /gene="rrrD"
                     /locus_tag="QIE41_004743"
     CDS             complement(858..1355)
                     /gene="rrrD"
                     /locus_tag="QIE41_004743"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDS3533511.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(1355..1570)
                     /gene="essD"
                     /locus_tag="QIE41_004744"
     CDS             complement(1355..1570)
                     /gene="essD"
                     /locus_tag="QIE41_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDS3533512.1"
                     /translation="MKSMDKLTTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(1764..>2214)
                     /locus_tag="QIE41_004745"
     CDS             complement(1764..>2214)
                     /locus_tag="QIE41_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3533513.1"
                     /translation="LIACLNCDGRTVDFVRNQISLACLAILSSEKIVQSFLFGCLNSL
                     GSKVKAIIHTDISAAWRLCDISSRLYLSESLLKRKLKHEGLSFSKLILEERMVMAERL
                     LSYNLYSVGKVAEICGYENTSYFVSVFRRYFGVPPHQYSSRFFLEKT"
BASE COUNT          628 a          514 c          445 g          627 t
ORIGIN      
        1 catcaatcca tttaactatc gtttaattgt tgtcacatag gattctgccg tttttaacaa
       61 tgcaggataa taagatgaaa aaaatgttgt tttctgccgc tctggcaatg cttattacag
      121 gatgtgctca acagacgttt actgttggaa acaaaccgac agcagtaaca ccaaaggaaa
      181 ccatcaccca tcatttcttc gtttcgggaa ttggacagga gaaaactgtt gatgcagcca
      241 aaatttgtgg cggcgcagaa aatgttgtta aaacagaaac ccagcaaaca ttcgtaaatg
      301 gattgctcgg ttttattact ttaggcattt atactccgct ggaagcgcgt gtctattgct
      361 cacaataatt gcatgagttg cccatcgata tgggcagctc tatctgcact gctcattaat
      421 atacttctgg gttccttcca gttgtttttg catcgtgatc agcctctctc tgagggtgaa
      481 ataatcccgt tcagcggtgt ctgccagtcg gggggaggct gcattatcca cgccggaggc
      541 ggtggtggct tcacgcactg actgacagac tgctttgatg tgcaaccgac gacgaccagc
      601 ggcaacatca tcacgcagag catcattttc agctttcgca tcagctaact ccttcgtgta
      661 ttttgcatcg agcgcagcaa catcacgctg acgcatctgc atgtcagtaa ttgccgcgtt
      721 cgccagcttc agttctctgg catttttgtc gcgctgggct ttgtaggtaa tggcgttatc
      781 acggtaatga ttaacagccc atgacaggca gacgatgatg cagataacca gagcggagat
      841 aatcgcggtt actctgttca ttgctgaccc cacaaacaga tttcacgctc aatctcacga
      901 cgagtcatga gacctttcca ttgcttaccg ccagcatatg tccagcgacg taactgatca
      961 catgcgcctt tgatatcgcc ctggtttatt ttgcgaagaa gcgtcgatgt tctgaaattg
     1021 ccagcaccca cgttgtaaac gaatgagtaa agagcgccgc gcgttgtttc cggtatatcg
     1081 actttgatgt acgggttaat ttgtctggcg acagtggcaa ggtctttatt caagagtgct
     1141 ttgcattctg ctttggtata cgttttaccg agcatgatgt cttttcctgt atgcccgtga
     1201 catacagtcc atacaccaac aatatctttg tatggtatgt agctgacacc ctccagacca
     1261 tcgttaccac ctggaccagt gattaacact gatgctatag caattgctcc gccaccaata
     1321 gcagcagcaa cggcttttcg taatgatgga ggcattattc acctctcgca gccttgcgct
     1381 tatcttcttt aatcttgaaa taaaggtttg tcaggtacgt cagcaggcca aataccaagc
     1441 tacccagcac acctattgct gcccactgtg agggcgtgac tttatcgagc agctgtaaaa
     1501 accagtagcc agcactgcct gcggaggtgc cgtaggcaat gcccgttgtt aacttatcca
     1561 tggatttcat agcctcacct ccgcaaataa cggatggtgt acacggttcg gaacgaagag
     1621 gaaaggtata gaagttacat tagcgtaagg cttgaacatc tattcaaaaa gaaaaacgcc
     1681 agcgattatt ctggcgtagc tgaaagcatc atacaattat caaatacgaa aattacaaaa
     1741 tcattaaaac gcatcacgtt acatcatgtc ttttctaaaa aaaatcttga tgaatattga
     1801 tggggaggaa caccaaaata tcttctgaaa acacttacaa aatatgacgt gttttcataa
     1861 ccgcatatct cagcaacttt tccaacagaa tataaattgt agcttaataa cctttccgcc
     1921 atcaccattc gctcttcaag aattaactta ctaaatgata agccttcgtg ctttaatttt
     1981 ctttttaaca gactttcact cagatacagt cttgaagata tatcacaaag tctccatgct
     2041 gcagatatat ccgtgtgaat aatagcctta actttacttc ctaaactatt aagacatcca
     2101 aataaaaaac tttgcactat tttctctgaa gataagatag caagacatgc aagtgatatt
     2161 tgatttctaa caaaatccac agttctgcca tcacaattca agcatgcaat caag
//
LOCUS       DAOTRK010000295         2168 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.298, whole genome shotgun sequence.
ACCESSION   DAOTRK010000295 DAOTRK010000000
VERSION     DAOTRK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.298"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..538
                     /locus_tag="QIE41_004746"
     CDS             <1..538
                     /locus_tag="QIE41_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533514.1"
                     /translation="KKHLLSLLYTNVITALETLYVELFINSIEKDDVYIANCIEKGKT
                     EFKVSKDIAALPFKGEPIEKIRGELIRSIKEHLISASWHSTKKVIDRYEATFDIKVQK
                     DCPIEAIELATLNRNHLVHRGGKDKEGNLVVITDQDLETLIENASNLAIMLYNSLNVA
                     TNKTTILQPDDKPFIHEF"
     gene            554..1555
                     /locus_tag="QIE41_004747"
     CDS             554..1555
                     /locus_tag="QIE41_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533515.1"
                     /translation="MDIRTRKTKFLESLDSTEVIRKAVSLAIDCIIDNHNSNEDTPLV
                     ITSYDDLCRIQVLNYVQEFCEAAFPDMDEYYFSPNILRINGKTSEEACINLIKLLRST
                     KGMLFWSDAPSWFASLPDGLFHVVNIDQKIVTRGLNKKNSKPTIINKDYSVDTLLSEL
                     FLNGAHMEQSNVHNVSEGNMKFYDECHAGLIRPIPAPIGASYDEEITINSPDWQKLAC
                     VALRRYQSKECHDGMQWDTTDHGWTDVIAYPFVEEIQSMDNSGYRQCLVGLVTINNSN
                     ANSPYLSTVWIHPFYRRRGLLSKLWPKLQELYGSNFEIERPNENMKAFLKSAKHADY"
BASE COUNT          701 a          408 c          470 g          589 t
ORIGIN      
        1 aaaaaaacac ttgcttagtt tgctctacac gaatgttatt acagctttgg aaacgcttta
       61 tgttgagcta ttcattaatt ctatagagaa agatgatgtc tacatagcca attgtataga
      121 aaaaggtaag actgaattta aagtaagcaa agatattgct gcgttgcctt tcaagggtga
      181 acccatcgag aaaattaggg gagagctaat caggtcaatc aaggaacact tgatcagtgc
      241 aagctggcat agcactaaaa aggtaatcga ccgctatgaa gctacattcg atataaaagt
      301 acaaaaagac tgtccgatag aagctattga attggcaacc ctcaatcgaa atcacttagt
      361 gcatcgtggt ggaaaggata aagaagggaa cctcgtagtg attacagacc aggatcttga
      421 aacgctgatt gaaaatgcat cgaacctggc aattatgctc tacaacagtt taaatgtagc
      481 aacgaataaa accaccattt tgcaaccaga tgacaaaccg ttcattcatg aattttaata
      541 gcgcaggtca tacatggata ttcgcacacg aaagacaaaa tttttagagt cgttagattc
      601 aactgaagtg atacgcaaag cagtaagtct cgctatagat tgcattattg ataatcataa
      661 tagtaatgaa gataccccct tagttattac cagctatgat gatctctgta gaatccaggt
      721 gctaaattat gtacaggagt tttgcgaagc tgcattccct gatatggatg aatattactt
      781 tagtcctaac atacttcgca tcaacggcaa gactagtgaa gaagcttgta ttaacttaat
      841 taaactctta agaagtacaa aaggcatgct attctggtcc gatgccccat cgtggttcgc
      901 tagcctcccc gacggattat tccatgttgt taacattgat caaaaaatcg ttactcgcgg
      961 ccttaataag aaaaactcta agccgacaat aatcaataaa gattacagtg tagatacact
     1021 actatcagag ctatttttga atggcgcaca catggagcaa tccaacgtac acaacgtttc
     1081 tgagggaaac atgaagttct atgatgaatg ccatgctgga ttaatcagac caatacccgc
     1141 tccaataggc gcgtcatacg atgaagagat cacaattaat tctccagatt ggcaaaagct
     1201 cgcgtgtgta gctttacgcc gctatcagtc gaaggaatgt catgacggaa tgcaatggga
     1261 tacaaccgat catggttgga ctgatgtaat cgcgtatcca ttcgtcgaag aaatacaatc
     1321 aatggataat agtggttatc gacaatgttt agttgggctg gtcacaataa acaattcaaa
     1381 tgcaaattct ccgtacctat ctaccgtttg gatacatcca ttttatcgca ggagagggtt
     1441 attaagtaag ttatggccaa aattacaaga gttatatgga agtaacttcg aaattgagcg
     1501 acctaatgaa aatatgaaag catttctgaa aagtgcgaaa cacgcagatt actaatttat
     1561 gaatcagtct gtggagttag ggttggtgtc aacaagtagg gtaagagcct caacgtgatg
     1621 ctatgccgac ctgctccctg agaattcaca cagaaggctg ctagcaatgt ctgcagatcg
     1681 ctcaaagcgg actagaaggt tagcttgcgt cggacttggc gtatttaaag aagtgctggt
     1741 ggtgactggt tgttgtgttc catttctaca gaacaaaatc acagaaacta tacccaatag
     1801 ttatattgaa tcaatgatga gacagcctca tatttatcag aactggtgta cgtccaatac
     1861 aggaggttgt cgtgctggtt ctcaaatgtg cgctagctat tgcggctgta atggcaattt
     1921 attgtcttgc tgttgttctt atggatcgcc tttctgattg attttatatt ggcgaggtga
     1981 cgggagttaa gtagaattgc tgcgggtgct tgaggctatc tgcctcaggc atgaacacca
     2041 aaggcagata gagaaaagcc ccagttaata ttacgcgtcc tgcaagacgc ttaacattaa
     2101 tctgaggctc aatctatgaa cggcaaatct aggttagcct cttacgtgcc gaaaggcaag
     2161 gagaagca
//
LOCUS       DAOTRK010000296         2126 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.299, whole genome shotgun sequence.
ACCESSION   DAOTRK010000296 DAOTRK010000000
VERSION     DAOTRK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.299"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..190
                     /locus_tag="QIE41_004748"
     CDS             <1..190
                     /locus_tag="QIE41_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDS3533516.1"
                     /translation="KVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMH"
     gene            205..1026
                     /locus_tag="QIE41_004749"
     CDS             205..1026
                     /locus_tag="QIE41_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDS3533517.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            1170..1245
                     /locus_tag="QIE41_004750"
     tRNA            1170..1245
                     /locus_tag="QIE41_004750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1203..1205,aa:Ile,seq:cat)
     gene            1347..1423
                     /locus_tag="QIE41_004751"
     tRNA            1347..1423
                     /locus_tag="QIE41_004751"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1381..1383,aa:Arg,seq:tct)
     gene            1923..2054
                     /locus_tag="QIE41_004752"
     CDS             1923..2054
                     /locus_tag="QIE41_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDS3533518.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
BASE COUNT          543 a          410 c          598 g          575 t
ORIGIN      
        1 aaaggtgatt gcagagccac cggataagcg tcgtcgcgac ctggacaata tcctgaaagc
       61 accgctggat gcgctgacgc atgcgggagt gttaatggac gatgagcagt ttgatgaaat
      121 caatatcgtt cgtggtcagc cagtatctgg tggacgtctg ggggtgaaga tttaccccat
      181 aatgcattaa gagcaggtca aaaaatgaaa ctggaagatt taccgaaata ctactcccca
      241 aaatcccctg gcctgaccga tgcatcggcc tcaacgtcaa aagatgcgct gagtatcact
      301 gatgtgatgg ccgcgcaggg catgacacag aatcgggctg agatgggttt ttctgcgttc
      361 ctggggaaaa tgggcatcag tatgaatgac agggcgcggg caacagaatt actggcagat
      421 tatgcactca gtcggtgcga tcgtgtggcg gcgttgagaa aacttccggc agaaataaaa
      481 ccggtagtga tgcgcattat ggcttcgtat gcgtttgaag attatgcccg tagcgcggcg
      541 agcaaaaaac agtgcccttg ctgtcacgga aaaaaattta ttgaaagcga ggtttttaca
      601 aacaagatcc agtatccgga tggtaagcca ccagtgtggg caaagtgcac aaaaggcgtg
      661 tatccgtctt actgggaaga atggaaaaaa gtcagggagg tggtaaaagt tgcctgtccg
      721 gagtgtggcg gaaagggtga ggtttccacc gcctgtaagg attgccgtgg gcgtggtgtc
      781 gccattcatc gtgaagagtc ggtaaaacgt ggtatgcctg ttatcagaga ttgccagcgt
      841 tgtggtggtc gtggctgtga aaggctgcca tcaacggagg catttaatgc catatgcaaa
      901 gtgacgagtg ctatcacgct tgatacgtgg aaaaaatcag tgaaacgctt ttacgatacg
      961 ttggtggttc ggtttgacat tgaagaggca tgggcggagc ggcagttaaa gagggtaacg
     1021 cgatagtgtt gttgattttt cccgaatctg tggtaaattc gtcataacga tgggcgtttt
     1081 atgcctggcg ttagaagagt ttctacaacc cgccgctgag cgggtttttt attgcgaaat
     1141 taattacgga ccgttattat tctgctcccg gccctttagc tcagtggtga gagcgagcga
     1201 ctcataatcg ccaggtcgct ggttcaaatc cagcaagggc caccatcaca aaccgccatt
     1261 agcttatcag gaagagcaga cgacacgata acagggttgt tggtgcgggg ggcgggtccc
     1321 cgatggcggt ccattatcgg tattcagcgt tgttagctca gccggacaga gcaattgcct
     1381 tctaagcaat cggtcactgg ttcgaatcca gtacagcgcg ccatattcat tcttccagat
     1441 tccttccggc agagccttat actgaaatat acctggctca ggatattgtt gaaaatatta
     1501 tatgtttgtc aaaaataaaa gttctgttaa gtattgattg aatatttgtt atacggtcta
     1561 atggtttttt cagcattaaa tatttatcat tcatatggtg tgggtagagt gaatattgat
     1621 gaggcgtcgg ggtgtttcat cctcaggcag cgtattgata tagtcaatgc agcacgagca
     1681 aaggccttca gccgtttgac agttttgttc tgtactcctg atcgtctttc gggaagagac
     1741 gttattattc tgaatagtga tgctatacag agggtttgcg atgagttcat ggttgctaat
     1801 tcagaattat ttgctcttgt tcaggagtac aacagaatag ccaggacctg tggtatggat
     1861 gaacttcgga ttactcatct ggggtagata catatctgga ttatcacctg ttacggtaaa
     1921 aagtgattgc ttactgtttt tgtgaatggc attgcagcag ccggataatg tcagtgctgg
     1981 ctgacggtgt gctggtggcg ggtgtggtgg ttgttgcttt cccgttgctg aaaaagaaaa
     2041 cgccagactg ttagccgggt atcagttagc gggagaaatt tttaaatact tcacaattca
     2101 ggcggttgac tgttgtctgg tttgcg
//
LOCUS       DAOTRK010000297         2122 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.300, whole genome shotgun sequence.
ACCESSION   DAOTRK010000297 DAOTRK010000000
VERSION     DAOTRK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.300"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1042)
                     /gene="aroH"
                     /locus_tag="QIE41_004753"
     CDS             complement(<1..1042)
                     /gene="aroH"
                     /locus_tag="QIE41_004753"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDS3533519.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDSR"
     gene            complement(1199..2032)
                     /gene="ppsR"
                     /locus_tag="QIE41_004754"
     CDS             complement(1199..2032)
                     /gene="ppsR"
                     /locus_tag="QIE41_004754"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDS3533520.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT          516 a          526 c          555 g          525 t
ORIGIN      
        1 agcggctatc taccgcagag gcgagttttt cgaccaggcg ttcggtatct tcccagccca
       61 gacacgggtc ggtaatcgat tgaccgtaag tgagcggctg accgccgacg attttttgcg
      121 ttccttcgcg caggaaactt tccgccataa ttccagcaat cgccgtagag ccattgcgga
      181 tttgctgaca aatatcctca caaacttcta actggcgacg gtgctgcttc tggcagttac
      241 cgtggctgaa atccaccacc agatgttcag gtaaatcaaa ctcgtgcagc gtatcgcagg
      301 ctgcggcgat atcatcggca tgataattcg gttttttgcc gccacgcata ataatgtggc
      361 catacgggtt gccgctggtc tgatagatgg tcatctgacc gtttttgtct ggcgagagga
      421 acatatggct ggcgcgggct gcgcggatag catctacggc aatccgcgtg ttgccatcgg
      481 taccattttt aaaacctacc ggacaggaga gtgccgaagc catttcacgg tggatctgac
      541 tttcggtagt acgtgcgcca atcgcgcccc aactgattaa atcagcaata aactgaccgg
      601 tcaccatatc gaggaactca gtcgcggtag ggacgcccag ctcatttacc tgtaaaagta
      661 atttgcgcgc cagctccaga ccgtgattta cccgatagct gccgtttaaa tctggatcgg
      721 agattagccc tttccagccg acaacagttc gtggtttttc aaaataggtg cgcattacga
      781 tttccagccg tgactggtac tggttgcgca gcgactgcag acgggtggcg tactccattg
      841 cagcggtgag atcgtggatc gagcaggggc caataatgac caacagtcgc ttatcttcac
      901 cattcagtat tttttcaatt ctgcggcggg agtcggtgac atgggtggcg acgccaggcg
      961 ttacgggata ccgtagcgcg agttcggcgg gcgttaccag gctctcaata cgcgcagtac
     1021 ggagttcgtc agttctgttc attacaagtc tcagaaattt gttgcttcta cggcaggact
     1081 accgggaagt gatgcgcatc acgataaacc aatcgcagct gatttcaagt gcgaggtgtg
     1141 tcacacctcg ccgggtggtt agcatgataa caaaaaaata agctaatgca ctagttctct
     1201 agtacattcg gcggctaagg cccatgatat cgaggatctt ggtggcaatc tcttctaccg
     1261 aataattggt actgttaatc cacgggatct gatttttacg gtacaaggct tctacttccg
     1321 cgacttccat cctgcactga cgaagcgagg catagcgact gttctcccga cgttcttcgc
     1381 gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa caatttatgc tgaagcggtt
     1441 tgagcgacgc gggtagcacc agattatcca tgtcgtcggc aataaagggg tagtttgccg
     1501 cgcggatacc aaattgcatt gccagataca gactggtggg ggttttacca cagcgagaaa
     1561 caccgagcag gatcacctga gcctgatcca gattgcgcaa cgaaatgccg tcatcgtggg
     1621 cgagggtgta atcaatcgcc gcaatgcgcg catcatattt attgagatta ttagggttaa
     1681 ggccatgggt acgatgagca atcggcgttg gatccagttt catctcttgt tgtagcgggg
     1741 caaccagcgc ctgaacgata tcctggcaaa agccttcact ttgcaagatg atggcgcgaa
     1801 tctccggcaa cacgatggag tagaagacca gcgggcgcac gcctgtctgg tgataaattg
     1861 cgtcaatctg atccttcact gcccgtgcac ggctctcatt ttcgacaaac ggcagcgtaa
     1921 tgctgctgat agtgacggga aattgtgaca ttactgcgtg tcctaatacc tccgcagtta
     1981 ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac agcattatcc attttgttct
     2041 tcccgtgatg cagacataat tctatgaaag cataaattaa aaacgcacag aagcgtagaa
     2101 cgttatgtct ggtttataaa at
//
LOCUS       DAOTRK010000298         2091 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.301, whole genome shotgun sequence.
ACCESSION   DAOTRK010000298 DAOTRK010000000
VERSION     DAOTRK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.301"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(60..1025)
                     /locus_tag="QIE41_004755"
     CDS             complement(60..1025)
                     /locus_tag="QIE41_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDS3533521.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1881..>2091)
                     /locus_tag="QIE41_004756"
     CDS             complement(1881..>2091)
                     /locus_tag="QIE41_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533522.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          579 a          552 c          435 g          525 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tccccaacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccccaaa acaagcaccc ccgaacggct taccccaacc
     1381 ttttccacac acccgatcac caaaacaggg aataaaaaca aaaccctgtc ttacaggtgg
     1441 atggcctcac ttcgtgaaac catccacccc gcataagttt tttaatcggt ggtgtcgtca
     1501 atcactaaat cggaacccca aaggggaccc ccacgattta gcatttgacg agattaactc
     1561 tcggagatgg catcatgctt ttaacggact ttaaaaaacg gtgaacgaat gcggatttta
     1621 atccgtgtct gtgaggcgtt gagcaacgag caaagcgagg cggtagaaag agagccagcg
     1681 gcaggctcag gacgataatc cggggaaaat gtaccactcg cagagtgtta acatttgaca
     1741 tcggattgtc tatcctgcat tggtgtcgag ttggcaaggc tgaggataat gctgaggata
     1801 atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca cctcagcctg
     1861 ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt gataccgttt
     1921 aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat atataaaatt
     1981 aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact taatccatca
     2041 ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct t
//
LOCUS       DAOTRK010000299         2036 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.302, whole genome shotgun sequence.
ACCESSION   DAOTRK010000299 DAOTRK010000000
VERSION     DAOTRK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.302"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..107)
                     /locus_tag="QIE41_004757"
     CDS             complement(<1..107)
                     /locus_tag="QIE41_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS3533523.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELC"
     gene            complement(494..792)
                     /gene="tnpB"
                     /locus_tag="QIE41_004758"
                     /pseudo
     CDS             complement(494..792)
                     /gene="tnpB"
                     /locus_tag="QIE41_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1303..2036
                     /locus_tag="QIE41_004759"
                     /pseudo
     CDS             1303..2036
                     /locus_tag="QIE41_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          556 a          480 c          505 g          495 t
ORIGIN      
        1 tcacagagct ccgcctcagc cggaagcggc gagcctggca cgtatttgcc gtgaacgatc
       61 tgtttaccca gcgtaatgac gatgcgatcg gttttattga gagtcatgga gagtccttgt
      121 gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa ctttgtagta
      181 ccagcgtgat aacgttcgcg tttgccgtgt gcgtaatgta gtacaagctt atattactgt
      241 actacaattt agatcacaaa aagaacaatg cataaaaaaa tgacatgcgt cgggcagaaa
      301 tggaaaaggg atatcaggcg ctaaacagga gggaaagaag agtatgcttt caacgactta
      361 gtactcagac agcagctttt ccagcgtcag cgatgcgaat gaggtagttc tgattcatga
      421 ccgtttttat aatccggcta tgatattttt accacattgt cagcgcgtta aggcgtgatg
      481 tttttggctg acgctagccc agcttactaa ggagcattgc cagctgcggg cgggtaaagg
      541 ataccccgcc gtcacgtacc gcaagccaga taaattgcct tcctcctggc gcttggtgaa
      601 aagacacaga ccatcagccc agagtatttt aacagtgtca ccccgtcggc ctcggaagat
      661 aaacaggtgg ccgaagaagg gattataatt cagcacatgt tgaccctgtt ttcccagtcc
      721 gttgaaggat ttacgcatat cagctacccc ggcaatgagg cagatacggg taccggatgg
      781 tagtgagatc atcgtctcct cctggctaac tcacggatta acaccttgag cagctctggt
      841 gaaggatttt ccaacacatc atgttactga gacggaactc aaccttacag gaactgacac
      901 tgactgtgtt atggggggaa atggataaag atggagcaat agacatcatc gtatttttct
      961 gttcattcgg cgttatctca acaggtaata atttaacgcc agcgtcagaa gtagtcgtta
     1021 caggaagacg ccgcgaaaca acacaggttc aggagaataa ggtagagcgt ctatagtcct
     1081 gtttcctcca tctaggatgt ctgatggaaa ttcatcagat acactaagga tccccataaa
     1141 gtattttatt ggcacacatc agacatcctt taggtatcca gcaagttatt gaactattgc
     1201 gtcgtctcgt tcaaacttct cttttattgc ctcaataata tatgagctaa aatccagaga
     1261 tgtttttgta gtggcacagt aaatttggcc acctgattaa aggtgatatc ctcaccacaa
     1321 cacaaaacag gtgacttaat gaacaagaaa accaaacgta ctttcacccc tgaattcagg
     1381 ctggaatgtg cacagctaat tgttgataag ggctactcat atcgacaagc cagtgaagcg
     1441 atgaatgtcg gttctaccac gcttgagagt tgggtgcgcc agctcaggcg agagcgtcag
     1501 gggattgcgc cctttgccac acctattact ccagaccagc aacgtatccg cgaactggaa
     1561 aagcaggttc gccgcctgga ggaacacaat acgatattaa aaaaggctac cgcgctcttg
     1621 atgtccgact cgctgaacgg ttcacgatag ttgccagact gagtgacagc cactcagttg
     1681 tcagcctctg ttctgctctg gaaatacacc gcagtagtta ccggtactgg cgaaaacgac
     1741 gcgatacggt taatccggcg cgagtcaggt tgtgcagcga aatacgccgg gcgtggaacc
     1801 aaagtagggg ctctgcgggg gcgcgcacgc tggctgaaat gctgacccaa aacggcgtcc
     1861 cgatgagccg ttaccgtgcc gggcgtctga tgaaatatct gaacctgagc agttgtcagc
     1921 ccggaaaaca tcagtacaaa aatgctcgtc aggaacatac ctgcctgccg aatctgcttg
     1981 agcgccagtt cgctgtgccc gagccagata gggtatggtg cggagatatt acgtat
//
LOCUS       DAOTRK010000300         2031 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.303, whole genome shotgun sequence.
ACCESSION   DAOTRK010000300 DAOTRK010000000
VERSION     DAOTRK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.303"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..831
                     /locus_tag="QIE41_004760"
     CDS             <1..831
                     /locus_tag="QIE41_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HDS3533524.1"
                     /translation="TDRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHR
                     GLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRY
                     YDPLQGRYITQDPIGLKGGWNFYQYPLNPISEIDPQGLNPLILSVVALMSCGLFSSGG
                     VIQSGQQRRDQYGRKVNPEADKLTDEHKSGVNPGGNCSPQNLSDLQNEKNRLCNQSRA
                     CAPQMLRKEILRRYEINLACASVRKQINNQCFGGGDKAHMEEENKAYKTAADCSGLIK
                     "
     gene            828..1331
                     /locus_tag="QIE41_004761"
     CDS             828..1331
                     /locus_tag="QIE41_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533525.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDITGLEELDIVYNF
                     FQKKQWNEIANNFKIKDDSYALELGITFLPEKVFCYYIPLYIYASLFNKNDFWVFESD
                     FIQQYLCPEYRDHDDFLNFVFNFSDIQLSIIAQFMSYESDAGFFYASKACMDFWEDYS
                     PLLHKKI"
     gene            1449..2024
                     /locus_tag="QIE41_004762"
     CDS             1449..2024
                     /locus_tag="QIE41_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDS3533526.1"
                     /translation="MHLYHCDYRGLPLTLISPDGATEWCAEYDEWGNLLTEENPQHLQ
                     QLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQEPIGLEGGWNQYVYASIHPTYSID
                     PLGLIDKPAPVFNRELNSDAYYLAVNNCYSYALNRYGNPGSRIFGGGGLQPGELSGKE
                     FSKLTCSSIFEASKNDGAKDRERSSEVLFLY"
BASE COUNT          607 a          400 c          474 g          550 t
ORIGIN      
        1 actgaccggg tgagcgagga aagccgccgc tggctggcat cgtgcggcct gacggtggag
       61 cagatgcaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca cctgtaccac
      121 tgcgaccatc gcggcctgcc gctggcgctt gtcagcacgg aaggggcaac agaatggtgc
      181 gcagaatacg atgaatgggg caacctgctg aatgaagaga acccgcatca gctgcagcag
      241 ctcatccgcc tgccgggtca gcagtatgat gaggagtccg gcctgtatta caaccgccac
      301 cgctattatg acccgctgca ggggcggtat atcactcagg atccgattgg gcttaagggg
      361 ggatggaatt tttatcagta tccgttaaat ccgatctcag agatagaccc acaagggtta
      421 aatccgttaa ttcttagtgt tgtagcactg atgagttgcg gtttgttttc atcaggtggt
      481 gttatccaaa gtgggcaaca acgcagagat caatatggcc gtaaagttaa tccagaagcc
      541 gacaaattaa cagatgaaca taagtcaggt gtaaatcctg gtggtaactg tagtccccag
      601 aacctgagtg acttacaaaa tgaaaaaaac agattatgta accagtctag agcttgcgct
      661 cctcagatgc tgagaaaaga aatattgaga cggtacgaga taaatttggc gtgcgcttcg
      721 gtcagaaagc agattaataa tcagtgcttt ggtggtggag ataaggccca tatggaagaa
      781 gagaataaag catataaaac agcagctgat tgcagtggct taataaaatg aaaaaaataa
      841 ttttctctaa cgagagtgca gtctatgatg aacttatgat tcatttccca tgccagccat
      901 tacctcatat ttcaaatgat attacagggc tggaggaact ggatattgta tataattttt
      961 tccaaaagaa acaatggaat gaaatagcca acaattttaa aataaaggat gactcttatg
     1021 ctttagagtt aggaattaca ttcctccctg agaaagtatt ctgctattac atccctctgt
     1081 atatttatgc ctcgttgttt aataagaatg atttttgggt ttttgaatcc gattttattc
     1141 aacaatattt atgtcctgag tatcgggatc acgatgattt tttgaatttt gtttttaatt
     1201 tttcagatat acaactatct attattgcac agtttatgtc ttatgaatca gacgctggat
     1261 tcttttatgc aagtaaagct tgtatggatt tttgggaaga ttattcacct ctattacata
     1321 agaaaattta atcgtaaaag gtataaatgg tttaataaaa aacttgtcta ctcgttggag
     1381 tagatgaaaa ataagatgac gttggcatat agatcactgc gaataatcca cctctaccac
     1441 tgcgaataat ccacctctac cactgcgact atcgcggcct gccgctgacg cttatcagcc
     1501 ctgacggggc aacagaatgg tgcgcagaat atgatgaatg gggcaacctg ctgactgaag
     1561 agaacccgca gcacctgcag cagcttatcc gtctgccggg gcagcagtat gatgaggagt
     1621 ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcgg tatatcactc
     1681 aggaaccgat tgggctggaa ggcgggtgga atcagtatgt atatgcttcc atacatccaa
     1741 cctattctat tgatccgctg ggcttaattg ataaacctgc tccagtattc aatcgagaac
     1801 taaattcaga tgcgtattat cttgccgtaa ataattgcta ttcatatgct ctaaataggt
     1861 atggaaatcc tgggagtagg atttttggcg gcggaggatt acaacctgga gaattatctg
     1921 gaaaagagtt ttctaaactc acttgtagta gtatttttga agcgtcaaaa aatgatggtg
     1981 ccaaggacag ggaaagatca tcagaagtac tctttttgta ctaaataatt c
//
LOCUS       DAOTRK010000301         2001 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.304, whole genome shotgun sequence.
ACCESSION   DAOTRK010000301 DAOTRK010000000
VERSION     DAOTRK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.304"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(513..1838)
                     /locus_tag="QIE41_004763"
     CDS             complement(513..1838)
                     /locus_tag="QIE41_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDS3533527.1"
                     /translation="MTVLQIVVLLGAIFLGVRMGGIGIGYAGGIGVLILGLCLDMKPG
                     NIPWDVILIIASVISAISAMQLAGGLDYLVQVAERILRKNPKYINYLAPVVTYVLTIF
                     AGTGHTAFSMIPVIVEVSKEQNIKPSCPLSIAVVSSQIAITASPVSAAVIYMSGVLEG
                     FGWSYPVLLGIWLFTTFVGCMLTAFIISLISDMKLDNDPVYRERLSKGLVSAPVKSVN
                     KQLKPYARRSVAIFLIGVILVVLYASAISPTLGLIDNVVVSRDAAIMSLMLLVGGFIT
                     LFCKADINKIADSSVFKSGMVACICVLGVAWLGDTFVSGHSGEIKELARTTVSQYPAL
                     LAVVFFLAAMLLYSQAATAKAITPAIVTALGITAANPDDSYMLVASFAAVSALFVLPT
                     YPTLLGAVQMDDTGTTRIGKYVFNHAFFIPGVLAIAFSVLLGFLVVSMF"
BASE COUNT          661 a          464 c          381 g          495 t
ORIGIN      
        1 gtgccgttct tttatagaaa ttcatatcta cctcctggac tgtgaaatat atttaccatg
       61 tatttttcta tagaacagtt aatgccttat aaatttgctt aataatgata tgttttaata
      121 aattcattct ctttaacttg tcattaactc atccagaata agatgataaa ctgttatatt
      181 tcaggatata atatccctcc ttgacaggtg ggcgaacaac attaactatc gataaaaaaa
      241 caccgcattc cgtgataaca tcattcatca aaaatattct tatcgatgta gaaaatatca
      301 atgtaaatag aatgtttaat tacctcccat tcagaatagc aatgagcgtc catcatgttt
      361 tttgattaga atcgtataat aaatgttgca ctaaaatatt tataacgaag taacaatgcg
      421 cacctcaata cttaaagatt gaatgggcta ccaatcaatg tggatacgta ttgacgggta
      481 gccaggagat tgaaattacg ttcggtcaag aatcagaaca tactcaccac aagaaatccc
      541 agaagcactg agaaagcaat agccagtaca cccggaataa agaaagcatg gttgaacaca
      601 tacttaccaa tacgggtcgt tcctgtgtca tccatttgca ccgcccccag aagggttggg
      661 taagttggta acacaaataa agcagacaca gcagcaaaag aagctaccag catgtaactg
      721 tcatccggat tcgctgcagt aatacccaat gcagtcacaa tagccggagt gatagctttg
      781 gcagttgcag cctgtgaata cagaagcatc gcagccagga aaaataccac agccagaaga
      841 gctggatact gggatacagt agttctggca agctccttaa tttctcctga atgaccagat
      901 acaaaagtgt cccccaacca tgccacaccc agtacacaga tacaggcaac catgcctgac
      961 ttgaacacag aggagtccgc aatcttgttt atatctgctt tacagaaaag ggtaatgaat
     1021 ccgccaacca gtagcatgag actcataata gccgcatcac gactaacaac aacgttatca
     1081 atcagaccca gcgtcgggct aatggccgaa gcgtaaagga ccaccaggat gacgccaata
     1141 aggaaaattg cgacagatcg tctggcataa ggcttgagct gtttgttaac actctttact
     1201 ggcgcgctta cgagtccttt ggaaagacgc tcccggtaaa ccggatcatt gtctaatttc
     1261 atgtcagaaa tcagactgat tataaatgcg gtaagcatgc aaccaacgaa tgtggtaaac
     1321 aaccatatcc caagcaatac tggataactc cacccaaagc cttccagtac gccagacata
     1381 tagataacgg cagccgatac aggtgatgct gtaattgcaa tttgagatga tacaaccgca
     1441 attgagagcg gacatgaggg cttaatgttt tgttctttgg aaacttcgac aattacaggg
     1501 atcattgaaa atgcagtatg tcccgtaccg gcaaagattg tcagcacata agtgacaacc
     1561 ggagccaggt aattaatata ttttggattt ttacgaagaa ttcgttcagc gacctgaacg
     1621 agatagtcca gtcctcccgc cagttgcatg gccgaaatag cagaaattac cgatgcaata
     1681 attagaatca catcccaggg gatattacca ggcttcatat ccagacaaag ccccagaatc
     1741 aataccccaa tgccaccggc ataaccaata ccaatcccgc ccatacggac acccaaaaag
     1801 attgccccca gcagaaccac tatctgaaga acagtgataa tatccatgat tctctcccga
     1861 attagtgaga cacctttacc cctgagctcc tctcaacaaa ggccttatgg aatgtatttt
     1921 tacaaacatc cgggcagagg actgatacgt tttcattcgc gccatcccct gccggatctc
     1981 ccttcttaca gattctgaac a
//
LOCUS       DAOTRK010000302         1985 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.306, whole genome shotgun sequence.
ACCESSION   DAOTRK010000302 DAOTRK010000000
VERSION     DAOTRK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.306"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..881
                     /gene="ansB"
                     /locus_tag="QIE41_004764"
     CDS             <1..881
                     /gene="ansB"
                     /locus_tag="QIE41_004764"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDS3533528.1"
                     /translation="LVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCD
                     KTDGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTA
                     ADKASANRGVLVVMNDTVLDGRDVTKTNTTNVATFKSVNYGPLGYIHNGKIDYQRTPA
                     RKHTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKS
                     VFDTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLAL
                     TQTKDPQQIQQIFNQY"
     gene            complement(1475..>1985)
                     /locus_tag="QIE41_004765"
     CDS             complement(1475..>1985)
                     /locus_tag="QIE41_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067029.1"
                     /note="response regulator in two-component regulatory
                     system with DcuS; phosphorylated DcuR activates
                     transcription of genes involved in anaerobic fumarate
                     respiration; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDS3533529.1"
                     /translation="SIHCKSDVIVISAADDSATIKDSLHYGVSDYLIKPFHISRLVDS
                     LTRWKHKKTLLESQKIFNQSELDLLTHKIIPNTEKIRHLPKGLSTDTLRIICRWIDEH
                     QHKKFTLNELASGIKISRISCRKYIIWLVSCHILAFDFSCGVTGRPAYRYWIRHQYYP
                     YIKKYSEKP"
BASE COUNT          583 a          427 c          437 g          538 t
ORIGIN      
        1 atctggttaa tgcggtgccg caactgaagg acattgcgaa cgttaaaggc gagcaggtag
       61 tgaatatcgg ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta
      121 acaccgactg cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag
      181 aaaccgctta cttccttgac ctgacggtga aatgtgacaa accggtggtg atggtcggtg
      241 caatgcgccc gtccacgtcc atgagcgcag acggtccctt caatctgtat aacgcggtag
      301 ttaccgcagc tgataaagcc tccgctaatc gtggcgtact ggtagtaatg aacgacactg
      361 ttctggatgg ccgtgacgtc actaaaacca acaccaccaa cgtagcgacc ttcaagtctg
      421 ttaactacgg ccctctgggt tacattcaca acggtaagat tgactaccag cgcaccccgg
      481 cacgtaagca caccagtgat acgccgttcg atgtctctaa gctgaatgaa ctgccgaaag
      541 tcggcattgt ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg
      601 cgggctatga tggcatcgtt agcgctggtg tgggtaacgg taacctgtat aaatccgtgt
      661 ttgacaccct ggcgaccgcc gcgaaaaacg gcactgcggt agtgcgttct tcccgcgtac
      721 cgacgggagc aaccactcag gatgccgaag tggatgatgc gaaatatggc ttcgtcgcct
      781 ctggcacgtt gaacccgcaa aaagcacgtg ttctgctgca actggctctg acgcaaacca
      841 aagatccgca gcagatccag cagatattca atcagtacta ataacctcgc cccggtaacg
      901 tgccggggct tttatagtat tggttttagg cagtttaatg aataaagtga cagacatgaa
      961 taatggggac catttcaata cataatggtt ttcttgtacc catgaaatgt ggttaattcc
     1021 ggatttatcg ggaggggctt gttaatgata tcctgatgct catcgtgatg atgagtaaca
     1081 ttcataaaat gtgcttatca aaaaccgtgg agtattactt ggcagataga tgagcgaatt
     1141 cacagttgaa tccagagaaa taatatcctc tgttcactgg tacaccaatg ttgttccagg
     1201 acaaattcat tcacgataaa attgcggaaa aaacgggcag gattggtaac gaagcatccg
     1261 ggagaatata ctctttctga acaatggggg agtgccatgc atcagtgtaa atgtcaaaaa
     1321 atctgaaatc ttccatattt accgcacata actattccct gaccatacgt ttatcctctt
     1381 aatatccgga acacactctc cagaaattat cgcacgatga gtttattgtg attaagtaga
     1441 tgtcataatt atggtagata aatgattaaa aggcttacgg tttttcacta tattttttaa
     1501 tatatgggta atattgatgt cttatccagt aacgataagc agggcgacca gtgacaccac
     1561 aggaaaaatc aaatgccaat atatggcaac ttaccagcca gatgatatat tttctgcatg
     1621 agattctgga tatcttaata ccactggcca gttcattgag tgtgaatttt ttatgttgat
     1681 gctcatcaat ccacctacaa ataattcgca atgtatctgt tgacagtcct ttgggtaaat
     1741 gtcttatttt ttctgtgttc ggaattattt tgtgagttaa taaatccagt tctgattgat
     1801 tgaatatctt ctgactctca agaagagtct ttttatgttt ccatcgtgta agcgaatcaa
     1861 ccaaacgtga gatatgaaat ggctttatca gataatcaga aacaccataa tgtaatgaat
     1921 ctttaattgt cgcagagtca tcagcagcag aaattacaat aacatcactt ttgcaatgaa
     1981 tgcta
//
LOCUS       DAOTRK010000303         1974 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.307, whole genome shotgun sequence.
ACCESSION   DAOTRK010000303 DAOTRK010000000
VERSION     DAOTRK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.307"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(74..1756)
                     /locus_tag="QIE41_004766"
     CDS             complement(74..1756)
                     /locus_tag="QIE41_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDS3533530.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          523 a          399 c          443 g          609 t
ORIGIN      
        1 agggcttggc tgtaacgtgc atcgctatct cgctcaataa ggcggcggaa aaatccgccg
       61 catgaaggtt tagttatttc gcttcgctta gtgctttctt gatattgtta aacttctcct
      121 gatttacctc gctaagcggt ggttggctgc tgtcgataaa ctctcttacc acgccttgca
      181 tctctttaac gacctgcgga ttggcggcgg caaggttatc tttttgctgt agatccgtca
      241 gtttgtatag acctaactga ttgttttcta ctgtatagac tagcgaataa tcgttatttc
      301 tcaccgtata agagaattgg cttaagtcct cggtgttggg gttatgcggg taatcgtctg
      361 actgatgacg gacaaatttg tggtaattat cccagaatgg aatattttcc tcatcaaacc
      421 agtgagaata agaggttatc caggtcagat ttttatgtgg ctcgccttgt ttcttatctt
      481 gcaaccaggg cagcaaggaa acgccatcca gcttaaggtc ttttggaatg ctgatatcgg
      541 ctgcatcaag agctgtcggg tagaaatcca ttgcggaaat cagcttgtca taattaccgg
      601 gttgaagttt tcctttccac cacataaaca ttggggtgtg agtaccgcca ggataggtct
      661 gactcttata gcctttttgc gccccgttca gcggcagagg accatcgata accgcaccat
      721 tatcggaggt aaagagaata atagtattgt catactgtcc gtttttcttc agttgttcga
      781 gaatgcgttt tacaccctga tcaacagaat aaacggaagc gtagtagtta tctgccgttt
      841 gactaccggt attaaattgc ttctgatatt gctccggtgc aggattatca tttggcaggt
      901 gcggagcatt ataagccagg taaagcataa aaggctggtc aagtgttttg gcacgatcaa
      961 caacgccaat tgcctcatcg gttaactgat cgctgatata accttttgcg gggacgcgtt
     1021 cacgattttt gaacagtgaa ggggagttgt aatatgccgt tcctgcagcg tggaatccca
     1081 taaagtaatc aaagccacgg ttttgaggtt gccattcttc cgcagaatag gtggtgaagt
     1141 tgtcatgata gtcacgcgtt tgtttatctt ccggtactgg cacattactg atttttgaca
     1201 agtgccattt acctactgct gcagtgtaat aaccatgatt ctggaataat tcaggcaaga
     1261 aagtttctgt tagcggaata ccatcctgag catcggtatt ggaatagaca ccaaagcggg
     1321 cgggcgctcg accggtcatt attgcggcgc gggaggggcc ggaaacaccg tgcgccacat
     1381 agccgttagt aaaacgtacg ccttcatcca ttaatgaaag gagcgtcggc gttgattttt
     1441 gtgcagcttc aatggcttta tctatcccta ttttgtaggt atcgacaact tcacgatttt
     1501 ccattgtttt tgggtcaaaa gatcccttat caaaaggaag ttgtccataa ccaagatcat
     1561 ccatggtcag tacgataata tttggctttc ctttggtact gtattcagtc ggcgtaaagt
     1621 ctgagaaagc gacgtttgtt ttggttgctt tcagctttac atcatctgcc gcatgagcag
     1681 caaatgcagc cataccagat gccagtatca aagatatcga ggtacttacg acacttttct
     1741 ttaatgcaga cttcataaat gttcctcttc ttattgtact taattccaaa gtaattttac
     1801 gaagagaaat aagtgaatgt aagtgaagtt agtcacataa agagatagca gatttagcta
     1861 aaaaaagaga aataacagtc cataaactgt tgacattact ttctattcta ctaagtaatt
     1921 tctcgcagat aaacaactaa tctattgata tttaatgaat tattgtattt tact
//
LOCUS       DAOTRK010000304         1974 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.308, whole genome shotgun sequence.
ACCESSION   DAOTRK010000304 DAOTRK010000000
VERSION     DAOTRK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.308"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(329..1201)
                     /locus_tag="QIE41_004767"
     CDS             complement(329..1201)
                     /locus_tag="QIE41_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDS3533531.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVLAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT          503 a          440 c          521 g          510 t
ORIGIN      
        1 tttccttaat cagagtgagg gtgaataaaa aagccggtat ccacagatgt ggataccggc
       61 aggtaaagga ttacagtgcc ggaggtccgg ctgacggaag tttctgacgt gtggctctgt
      121 tgttccctga atctcatgtc tgtgtgtatc tgcgcacgtt gctaacagga gttattatta
      181 gcatgacgat cgatataaac gatcgataaa ataagtatcg atcgttttat tctattatca
      241 ggctattgca cggacaagag agacagtaac gaaaacagcg cttttgtgag cagactcaga
      301 ggcagggagg acatcatcca caacaggttc agaagaagat ccagttccat accgcgcggg
      361 caacagagac cacggtgtca cgaacagcgc gcagggccgt acggacaaca gaggcaacac
      421 agacgctctc cgctgtgtca aatatccggt ccaccgcacc ggtaaactgt tcacgggcct
      481 gagagcggac agactccgtg cgcagctcgt cctgcagtcg ggtcatcagg gggctggcgg
      541 catggtcggg aagcgctgtc atgagcgcac tgaccagcgt atccagttcc cagccggtgc
      601 gggccgatac ggcaacaacc ggatgtacgg gccggaacag ccggaatacc gcctccgttt
      661 tctcacgaat gttctgttcc tgtgcgggag aaggctgaat acctgccata tcccattcat
      721 ggcagggttc cgttttgtca gcctgcgtca ccacaaacag cacctgctga tgtccgcact
      781 gcaggatgtg tcgccagaaa tactcatcca cagacagggc gcggtcatcg gctttaatca
      841 gccacagtac caggtccagt tcaggcagaa tgtcacggta cagggcttca tactctgcat
      901 ccctgtcccg gctctcgccc accccgggca ggtcagtgat aaccatgctg tgaccgtgac
      961 cactcagacg gaagcgctgc acttcccggg tgccggcgag aacatcactg accggtgtga
     1021 cctccccctg aaacagtgca ttacagagtg aagatttacc ggccccgctt ttacccataa
     1081 tgccaatcac gggttcgtga ctggtgagtt tgcgcagatg ttccaggatg tgacgggaaa
     1141 gtgagtaagg cagggaggag agcggttttt caattgcctc aatggcatca gacggattca
     1201 tacatgtatt cccggtgaaa aaagacaaaa ccccgcgcac cggaaaggtg gcgggggatt
     1261 cacggagata atatctgtct gaaattgttt ggaatcgatg gggatgttgc gtattaacat
     1321 gaaggaggac aggagttatc cgttattggg cgatatcctt ccatgaaaat acgcggttat
     1381 tgttaaacag caggctgatt actccgtaaa ggagaaaaat attcatcttg cagcctgatt
     1441 atcaggtgtg tttatgttta ctgaggtgag tatttttcag tatattgtcg gggcagtcag
     1501 tgggagatat taaatttacg tttagagtta tcttgcttct gctgtgtatt tttataagag
     1561 tgcgcacgta atgatatctg ttgataccat gttgttaagt gtttcccttc cgggtcatgc
     1621 gtacttgcaa aatggcatct gaatcgatca cggtttggcc aaaccgtgat cgaatagaga
     1681 ggaatacgca cgtaatgatt aaatgctttt gttacaccat tcttccggca tgaatattta
     1741 tccagttccc ttttttattt gtgtagtgat actgtcggaa tagcgttcag taatgtgaag
     1801 tgtcctgtta aaaatcttta ataaatgtgt catcctggtc aggaaatatc tgctgagata
     1861 ttattagttg gtggtgcttt tactgagtaa tagctggctt ttaattcctg aactgaaata
     1921 ctgtcgctcg tcagggttta attaaaactg gatgatgatg tgaaaatgaa ggct
//
LOCUS       DAOTRK010000305         1946 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.309, whole genome shotgun sequence.
ACCESSION   DAOTRK010000305 DAOTRK010000000
VERSION     DAOTRK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.309"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>1946)
                     /locus_tag="QIE41_004768"
     CDS             complement(<1..>1946)
                     /locus_tag="QIE41_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDS3533532.1"
                     /translation="TVRVTAKVMDKTFTLSIKQTAATEPDSEVSAVLTAAPAEQVVGY
                     NINLQLAVKDSQGNAITGDNTLSFYALNQAEGVEFGAVTEKDGVYSATVTSKRAGKIR
                     IGVKSGSHNFSGIEKEISFIEDRTQFAFSRIEASKNNALADGKQQNTVTLSLADRFGN
                     VVPGYAVTLSLPAGVTQVGGEHAVSTDENGNAVFALISSTPGSYVIKAHAGSQMSTEL
                     TVTFASNMIGASLSLTPGNSSLISNIPANGKDAAVLNVQLTNTNASVNGQKIQLITSS
                     ERLSVPTNIVTDSTGHVSVPLTTVRAGEYTVAARVTDGSHSVESGSVKLTFVPDAASA
                     ELTMSTSKQQIVADGSESATVDIQLVDANNNAFTGDVDLTVTPSTGASLTSSKLQLDA
                     HGQATTQFTASKSGQYTIQAEYVLDGKRITASKNIDAVTDVKEAVLEITSSASSAVVS
                     DTNNLIFTLLLKSTSGEVLSGRVLNIKASGPSKYGALVVDKTTVTTDEYGLATVSVHG
                     RTAGSYKLTATLDELGSDVSEVKSFSLYADEANGVLTLHKDGGYDTDDGTPVGVYARF
                     VDHFGNPLSGTVEFSAGSEDSPNSQRVKMEPATVTLHWTGNAASEFSTYESGYHWIKA
                     KITNSKNTYEKTIRTYVVKLPEKD"
BASE COUNT          513 a          490 c          374 g          569 t
ORIGIN      
        1 ctatcctttt cagggagctt tacaacataa gtccttattg ttttctcata tgtatttttg
       61 ctgttcgtta ttttggcctt aatccagtga taaccgcttt cataggtgct gaactccgag
      121 gctgcattac ctgtccagtg taacgttact gtagcaggct ccattttaac ccgttgagag
      181 tttggactat cttcactacc agcagaaaac tcaaccgtac cagataacgg attgccgaaa
      241 tggtcaacaa atcgggcata aacccccact ggagtaccat catctgtgtc atagcctcca
      301 tctttatgca gtgtcaatac tccatttgcc tcatcagcat acagagagaa tgacttcacc
      361 tcactaacat cactgccaag ttcatctaag gtcgccgtta atttatacga accagccgtt
      421 cgaccatgta cgcttaccgt agccagacca tactcatcag tcgttacagt ggttttatca
      481 accaccaacg caccatattt ggacggccca gatgccttga tattcaatac tcgaccactt
      541 aatacttcgc ctgacgtact tttcaacagt aacgtaaata tcagattgtt ggtatcgctc
      601 actacagccg aagatgcaga tgatgtgatc tccaacacag cctccttcac atccgtcacg
      661 gcatcaatat ttttgcttgc ggttatacgt ttcccatcta gaacatactc agcctgaatt
      721 gtgtactggc ctgatttcga tgctgtgaac tgcgttgttg cctgtccatg agcatccaac
      781 tgcagtttac tactggtcaa tgatgctccc gtcgatggtg taaccgttaa atccacatcg
      841 cctgtaaacg cattgttatt ggcatcaacc aactgaatgt ccactgttgc actttcacta
      901 ccatcggcca caatctgctg ttttgatgta ctcatagtaa gttctgcgga agcagcatct
      961 ggtacaaaag ttaacttaac gctgccagac tccacactat gggaaccgtc agtcactcgt
     1021 gcagccaccg tatactcacc agctctcacc gttgtaagtg gtacggaaac atgccctgtt
     1081 gaatcagtca cgatatttgt cggtacagac aatctttcag atgaggtgat gagctgaatc
     1141 ttctgtccat tgaccgacgc attcgtattt gtcagttgca cattcaatac cgcagcatcc
     1201 ttaccattag caggaatatt ggaaattaag ctactgtttc caggcgtcag tgacagtgaa
     1261 gctccgatca tatttgatgc aaaggttact gttaactcag tagacatctg agaaccagca
     1321 tgagctttaa tcacgtaaga acccggagtg gagcttatta acgcaaaaac agcattaccg
     1381 ttttcgtcag ttgacacagc atgttcacca ccaacttgag ttacccccgc aggtaaggac
     1441 agcgtaacag cataaccggg cacaacattg ccgaaacgat ctgcaaggct taaagtcacc
     1501 gtattctgtt gtttgccgtc agccaatgca ttatttttac tggcttcaat acgagagaat
     1561 gcgaactgtg ttcgatcttc aataaaggag atttctttct caatacctga gaaattgtga
     1621 ctacccgatt taacaccgat ccgaatctta ccagctcgtt ttgaagttac tgtcgcgcta
     1681 tagacaccgt ctttttccgt tacagcccca aactcgactc cttctgcctg gttaagagca
     1741 taaaagctca gagtgttatc accagtgatt gcatttcctt gtgaatcctt cactgccagt
     1801 tgtagattga tattgtaacc aactacctgt tctgctggtg cagcggttaa aacagcactg
     1861 acctcagaat caggttctgt tgctgctgtt tgcttaatac tgagagtaaa tgttttatcc
     1921 attacttttg cagttacacg taccgt
//
LOCUS       DAOTRK010000306         1916 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.310, whole genome shotgun sequence.
ACCESSION   DAOTRK010000306 DAOTRK010000000
VERSION     DAOTRK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.310"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1911
                     /locus_tag="QIE41_004769"
     CDS             <1..1911
                     /locus_tag="QIE41_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HDS3533533.1"
                     /translation="VFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEAD
                     AGEIITSFGEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQ
                     MSIDADLRAGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIG
                     GLSVGVGQNGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQ
                     NIMSQLLSNSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGVEETV
                     VEGDITVGLEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIR
                     SQFFIEYGIRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVST
                     LIKKPVIVNSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGI
                     QETKYILDQLEMKYPDLLKEILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWS
                     PREKDIITLVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLN
                     LDASEAEMILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGEL
                     TENVPVNILKTI"
BASE COUNT          543 a          306 c          423 g          644 t
ORIGIN      
        1 gtgtttttag gatcattcta tgttgatagg atcttaagtt tttcttcatt cccatcaatt
       61 cttctcatca caacgttatt tcgtttggcg ctggcgatca gtaccagccg acttatactt
      121 cttgaggcgg acgccggaga aattataacg agttttggtg aattcgttat tggagatagc
      181 ctggtagttg gttttgttat tttctccatt gttactattg ttcagtttat cgtgattact
      241 aaaggttcgg aacgtgtggc tgaagttgct gcccgctttt cgcttgacgg tatgccaggt
      301 aaacaaatga gtattgacgc cgatctacgc gccgggatca ttgatgccga tttagcaaaa
      361 gaacgtcgta gcgtattaga acgagagagt cagctgtatg gttcctttga tggtgcgatg
      421 aaatttatca aaggtgatgc tattgcaaac attattatta tctttgttaa tattatcggt
      481 gggctatcag taggagtagg ccaaaatgga atggatttct cgacagcgtt gaccgtctac
      541 acaattctta ccgtcggtga tggtcttgtt tcacaaattc cagctttatt aattgctatt
      601 agtgcagggt ttatcgtaac gcgcgtgaat ggcgatagtg ataatatggg gcaaaatata
      661 atgtcccagt tattaagtaa ttcttttgtc attattgtga catgtgtcct cgcgctaagt
      721 attgggttgc ttccaggttt tccccttctt gtgtttttat gtttggcagt aatcttagga
      781 atttattttt actttaaatt caaaaagaaa ggggttgaag aaacggttgt tgagggcgat
      841 atcaccgttg ggttggaacc ctataatcag gatgatgata tttcattagg tattattaat
      901 aaacttgatc aggttattac cgaaacggta cccttggttt tgattatgaa tagtgtacag
      961 gctaaaaaat acactgaaat caatcttgct gataggattc gtagtcagtt ttttattgag
     1021 tatggtatcc gtattcccgg tattgtcatt cgcgaggggg aaggccttaa tgatgaagat
     1081 gtcatattaa tgctaaatga agtcagagcc tcgcaattta aaatttatca tgatctcgtc
     1141 ttgttggttg aatattcaga tgaagtggtt tcgactttga tcaagaaacc tgtcatcgta
     1201 aatagtaatg gtgaacaata ttattgggtt acaaaaagtg atgcccaaaa attaacaaaa
     1261 attggctgtt atactcgcac ggccatggat gaaatgtaca atcatttatc tgtatgtctg
     1321 gcgcataata ttaatgagta ttttggtata caagaaacca aatatattct tgatcaatta
     1381 gagatgaagt atcctgatct attaaaagaa atattaagat atataaccgt gcagcgcatt
     1441 tcagaggtaa ttcagcgtct gatccaggag cgaatttcag tgcgcaatat gcgtttagtt
     1501 atggaggctt tagcattatg gagtccacgg gagaaagata taattacact cgtcgagcat
     1561 gtacgaggtg cattaggtcg ctacatttgc cataagtttt catattctgg agaaattaaa
     1621 gcaattgtga tttcgcctga aatagaggac agaatcagag atggtgtcag gcctaccgct
     1681 ggcggtactt ttcttaatct cgatgcatcc gaagctgaga tgattctgga taattttaaa
     1741 ctggcacttt ctggaatcaa tattcctatt aaagacatta ttttactggg atctgtggat
     1801 attcgtcgtt ttatcaaaaa acttattgaa tctagttatc gagacctcga agtactttcg
     1861 tacggggaac taacggaaaa tgttcccgtt aatattctga aaactattta ggataa
//
LOCUS       DAOTRK010000307         1886 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.311, whole genome shotgun sequence.
ACCESSION   DAOTRK010000307 DAOTRK010000000
VERSION     DAOTRK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.311"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            199..453
                     /gene="bssS"
                     /locus_tag="QIE41_004770"
     CDS             199..453
                     /gene="bssS"
                     /locus_tag="QIE41_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDS3533534.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            568..1686
                     /gene="solA"
                     /locus_tag="QIE41_004771"
     CDS             568..1686
                     /gene="solA"
                     /locus_tag="QIE41_004771"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDS3533535.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1734..1847
                     /locus_tag="QIE41_004772"
     CDS             1734..1847
                     /locus_tag="QIE41_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDS3533536.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          496 a          449 c          444 g          497 t
ORIGIN      
        1 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
       61 taaaagcaaa aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
      121 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
      181 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
      241 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
      301 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
      361 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
      421 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
      481 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
      541 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
      601 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
      661 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
      721 catgcttatg gtgaaggcga aaagtatgtc ccgctggttc tccgcgcgca aacgctgtgg
      781 gatgaactct cccgccacaa cgaagaagat cccatttttg tacgctctgg cgtcattaac
      841 cttggcccgg ctgattccac atttctcgcc aacgtcgccc acagcgccga acaatggcaa
      901 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccgga aatacgcgtc
      961 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
     1021 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcagctgtt caactgcccg
     1081 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggctga cggtgagtat
     1141 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
     1201 cctgtccagc ccgtacgcaa agtatttgcc tggtatcagg ccgatggtcg ctatagcgtg
     1261 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
     1321 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
     1381 gcggatgaac gtgttccgtt tgcggaagtg gtcagcgatg gttcggaagc cttcccgttc
     1441 ttgcgcaatg tcttgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
     1501 aattcgcctg acgaagattt tattatcgat actctacccg gccacgataa tacactgctc
     1561 attaccggtc tgagcgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
     1621 gattttgcgc aagacaaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
     1681 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
     1741 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
     1801 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
     1861 cataaactga caaatattta atctcc
//
LOCUS       DAOTRK010000308         1844 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.312, whole genome shotgun sequence.
ACCESSION   DAOTRK010000308 DAOTRK010000000
VERSION     DAOTRK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.312"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(153..1796)
                     /gene="yeeO"
                     /locus_tag="QIE41_004773"
     CDS             complement(153..1796)
                     /gene="yeeO"
                     /locus_tag="QIE41_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDS3533537.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFDGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          571 a          499 c          385 g          389 t
ORIGIN      
        1 cagtgacata cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgag
       61 aacgaggcga atattagcga tgcccaccca caatgtcaaa gcctgttttt taaatttgaa
      121 atcgtttgct gaaataatct gcattttgtc gtttattccg acacaactgg ctttttttca
      181 cacttttgcg gctcggatcg agggtatttc catagccaac gtccagtaac cattcgccag
      241 taaaacagca cggcccgcac agcccagtcg gcaaacattc ccatccagac gccaaccaca
      301 ccccagccaa gcatgattcc cagcacataa ccgactacaa cccgacaacc ccacatgctc
      361 aacatcgaaa cccacatggc gtaacgggca tcacgagcac ctttaaatcc agcgggtagc
      421 acccatgagg cggaccaaat aggcataaat aaagcattta gccaaatcag aatcacaacg
      481 acgtgtttaa cctgtggatc ctgggtgtaa aacgatgcca taaccccggc aaagggagcc
      541 gttagccagg cgatggccgt taatccaaga gtggacagcc agaacacatg ccgcaactga
      601 atctctgctt gcgctatctg ccctaccccc aaccttcggc ctgtaatgat cgtagaagca
      661 gaaccgagcg cacttccggg taagttgata agagccgcaa ttgagaacgc gataaaattt
      721 ccggcaataa cgctggtccc catcccggca acgaacattt gggttaataa ccgaccactg
      781 gtaaataaca ctgattcgac actcgctgga ataccaatcc ccatgacttc ccagataatg
      841 ctaaaattca gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg attaaaacca
      901 atcgccagca cccacaaaat tgcaactgcg ccaatataac gagaaatggt taaacccaac
      961 ccagccccga caaatcccag tcccggccag gagaaaaggc cgtaaatcaa tatgccgcta
     1021 ataataatat taagaatatt caggctaccg ttaatcagta gcggtatttt cgtattccct
     1081 gcaccacgaa gtgccccgct accaataaga gtgatggcag ctgctggata actgagtacg
     1141 gtcagctcca gataagtcaa cgccagtgct ttaacgtctg tcgtggcatc acccgcgacg
     1201 aaatcaataa tttgttcgcc aaaatgatga attagcgttg ccaacagtac ggcaaacaac
     1261 gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc gtcgtcgatc tcgcttaccg
     1321 agactaaatg ctacaacgac agtagtacca agatcgatag ctgcaaaaaa agccataatg
     1381 accatattga agctgtccgc caatcccacg ccggccatcg catcttttcc cagccagctg
     1441 accagaaaag tgctcagaac ccccatcaac aggacacagg cattctccat gaagatagga
     1501 acagcaagcg gggttatctc gcgccagaac aacactttgt agctcttgcg tttagcgtgc
     1561 cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat
     1621 tgcagaaagt gaaaccacat ttcaaataat gagggagaat cagcaaagct gcaaagattt
     1681 tcgccatcaa attgtctgca aatgcaacaa actgttgata gaaacggcaa acagttggga
     1741 aaattaaaaa tcgggtttga caaaagattt ttcgccgtta agatgtgcct caacagcgat
     1801 tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgta
//
LOCUS       DAOTRK010000309         1825 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.313, whole genome shotgun sequence.
ACCESSION   DAOTRK010000309 DAOTRK010000000
VERSION     DAOTRK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.313"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..122)
                     /locus_tag="QIE41_004774"
     CDS             complement(<1..122)
                     /locus_tag="QIE41_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDS3533538.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGA"
     gene            complement(826..1293)
                     /locus_tag="QIE41_004775"
     CDS             complement(826..1293)
                     /locus_tag="QIE41_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDS3533539.1"
                     /translation="MKACLLLFFYFSFIFQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQLRYLERSASQGSVTAQFNYGVFLSD
                     TNPILSEHYNLNKAIYWMEFAVNNGNIDAKSKLQDLKKLKLKRMDRRKNKENP"
     gene            complement(1305..>1825)
                     /locus_tag="QIE41_004776"
     CDS             complement(1305..>1825)
                     /locus_tag="QIE41_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDS3533540.1"
                     /translation="PVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPT
                     SIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNVI
                     GPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISN
                     MQGTPEKPSDIR"
BASE COUNT          549 a          371 c          302 g          603 t
ORIGIN      
        1 gcacccgaaa taacggcaca aatagtcaac agtagaatat ccgataattt atgttccact
       61 ttccaggctt gtctgtaatc ggggataata gaaatatgct ccatcaattt tttaagttcc
      121 attttgttct ccttaattat gtaaggagta tttgatcatg tataagcaat aaaaaacagc
      181 tttaggtaat gaggaatatc tcaattttca aacataaaat gcgaattatt tagtacaaaa
      241 aagcagcgtt acgtgatctt tccctggcat gtcattagat gaaccagtta tacaaccctc
      301 ggaacagaat cgaggtccta aagacggacc aaaagcgttt aaacaactat gtattgaaaa
      361 tccattacga ttataagttc ctgtattaac tgacggtact aatcgtatgc cgtcaggttt
      421 tctattgctt gctcccgggt cattaacatt ccaactccat aggccttgag gtattggccc
      481 tctatttata aatgtgtaca acggggatta tttttacatt gcatattatt accagaagcg
      541 actggaatac taagcacatc ataaaaatta tcatcacctg gtatatctga aacacacgaa
      601 agcataccta aagttatgct gtataaacag gtggctaatc ccaaggggat cgattccaga
      661 aatcgggttc aacggatact gataaaaatt ccacctacct tccagcccaa tcggagcctg
      721 agtgttatac cgcccctgct gcggttaatg ttaccgataa tagttatagt acagccctga
      781 ttcatcatca tactactgct gcggcagtct gatgaattat tgcttttacg gattctcctt
      841 attcttcctt ctatccattc gctttagctt tagctttttg agatcttgaa gtttgctctt
      901 tgcatcaata ttaccgttat ttacagcgaa ttccatccaa taaatagctt tattcaaatt
      961 ataatgttct gataatattg gatttgtatc cgataaaaaa accccataat taaactgcgc
     1021 tgtaacactc ccctgagatg ctgatctttc taagtatcgc aattgtttgt aaatgttatt
     1081 tttagtaaag cagtaatatt gatatagtcg aaatgatgcc tctgaatcac catgttgtga
     1141 tttatacttc agtttcatca acttttcttc acttaaatca taggtcattg ctgtagaccc
     1201 catcatactt tcgttttgtt ttattttcac atcagcacca tgcaattgaa aaataaaaga
     1261 gaaataaaaa aatagtaaca agcatgcttt catacatcta caacctatct tatatcactg
     1321 ggtttttctg gcgtaccttg catattagaa atggggtgcc cagatggccc atatgttatc
     1381 caattaccaa ggtcttttgt tgttatagcc attagatcct tatttgaatt aaggttcgtt
     1441 ggtgctgtta gcactgtatc catttttaac ttaccattag ttcctcgccc ccaccacgtc
     1501 catagcgtgt caggtcctat tacatttgta tgtaactctt tagctaattg gctagcaaat
     1561 gaattttgtc ctttacctgt attacaagaa aataaccaaa ctgtcatccc atctttataa
     1621 ttcccatcaa atttaattag atatgctaga tctttagctg tcatgatact gcgcgttgcg
     1681 gattcaatag atgtgggggt accatgcccc ccgattgtga aaacgcctgg gatattaatc
     1741 tcatcagcta cagaatggat aagatcactt tcggggtata aatttatatc aaccaaacct
     1801 tgcggatcta catttatgac cggat
//
LOCUS       DAOTRK010000310         1791 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.314, whole genome shotgun sequence.
ACCESSION   DAOTRK010000310 DAOTRK010000000
VERSION     DAOTRK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.314"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(133..675)
                     /locus_tag="QIE41_004777"
     CDS             complement(133..675)
                     /locus_tag="QIE41_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDS3533541.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKTA"
     gene            complement(672..962)
                     /locus_tag="QIE41_004778"
     CDS             complement(672..962)
                     /locus_tag="QIE41_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDS3533542.1"
                     /translation="MVDLRKAARGRMCTVRILGYCNHDPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEDLRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(962..1561)
                     /locus_tag="QIE41_004779"
     CDS             complement(962..1561)
                     /locus_tag="QIE41_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDS3533543.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          466 a          478 c          377 g          470 t
ORIGIN      
        1 tctgatatca caccacagaa acaacaaaac ccgctcgatg gcgggttcta ttaaagttta
       61 attgcgcttg attcgcctcg cgatacagct ttgcgaagcg tagcaaaatt gaagcagttt
      121 atgcgtaaaa aatcaagccg ttttttgagc gaatgattct cgcatgggaa tgtatagcgc
      181 atactcagca acggccaacc aattagcaat tcgcttttcg catgtgctaa aacaccactc
      241 tgggtgtgca tcatttagca attcagccat tttgcgtttg gtcatccccc gcccctcata
      301 ccgttgccga aggacgctaa tcaatcctgg atgctctgcc agcacctcac ttatgacccg
      361 atcaatacat aacgcctctg catcagtaca atgcgccagc cagctctttt gcttaccgtt
      421 aatcatatcc cgcaaaaaag cctcaagttc agacttgttc agacctgctt ttttcatcct
      481 ccggagcgcc tcgttaattg ccgtttttgt cagcttttta gaggccaaca actggttgaa
      541 catattcccc gtcttaccgc cgccaatata cgaccagcgc ccccacatgc gcagttttcc
      601 ctgaatccag acactttcca gcgtggtgag acgaaggtgt tccccgcttt tgcctgtatt
      661 tgttgggtaa atcataaata accttccttt ctccagattt cttgcgtgcg aaaaacacct
      721 tctgcatgca tcaggcgtaa atcttctttg gtgtaatcgc ttgtttttac ccgcccgtcg
      781 attaaatcgt ggcatgagct acaggcaatc gccgcctgca tatcgtgtgg ttttgtcgct
      841 gttccgcacg ttcccgccag tcggtaatgc gccagcacag acgtttccgg atcgtgattg
      901 cagtagccaa gaattctgac ggtgcacatc cgcccccgcg ccgctttacg taaatccacc
      961 attacgcaaa ctccagcagc tgcgcggcca cattttcgac ttgttccgga gaggaaaatt
     1021 tacggaacag aatccagttc cacagcacat tcagaacaga tttataaacc tgctgaaact
     1081 cggtttcgtc catattcgca aaagcgatag attttgcccg acgcccgcgg ctaccgtccg
     1141 gataaaaatg ttcggtgtaa aatccagcct gaatggttac ccactcgcga aaagcctcaa
     1201 acgactttag caatgccgta tcccgggttc tgcgtgtcgc aactgtattc agatattgct
     1261 ctgcggcatc actcagggct ggcgtgtgtt cccgaccaac tgattcgcac aggtaatcaa
     1321 cgaaaccaga cagcagttct cgttcgcgag gcgtgatcgc cccaccgacc ggagtccagt
     1381 aatcgaatcc cagttgcagg agtttgaaaa aacgcttgtg gaatgcgtag ttacgcacac
     1441 gcttaaagtc tgcgtgtatc cactcgccta ttttgatttg atgcagaaaa tcacaactct
     1501 ccggcgtcgc cgggagaagt aatccggaag aggtttgttt gaccagttgt atatgcgcca
     1561 tttcttaatc tctcgatggc gcagcgcagc agatgccagt tgttcaggct gacgtatgaa
     1621 gtataaataa actggctcca gtgtaaagcc ccaccttaat ggaataaaaa ccaaaaaaca
     1681 gattgctggg atacaaacaa cgcttattat taaaagcggt taaacaaatt aaattttaat
     1741 gttatgcaaa tctgccagat caccataata tctcatttga aaaccgctga a
//
LOCUS       DAOTRK010000311         1777 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.315, whole genome shotgun sequence.
ACCESSION   DAOTRK010000311 DAOTRK010000000
VERSION     DAOTRK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.315"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1130)
                     /locus_tag="QIE41_004780"
     CDS             complement(<1..1130)
                     /locus_tag="QIE41_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533544.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQSGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTG"
     gene            complement(1251..>1777)
                     /locus_tag="QIE41_004781"
     CDS             complement(1251..>1777)
                     /locus_tag="QIE41_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDS3533545.1"
                     /translation="GWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVK
                     FGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTF
                     SSRGDMRVGTSTAGSGMTFSPSQNGTSLVLQAGLEARVRENITLGVQAGYAHSINGSS
                     AESYNSQATLNVTF"
BASE COUNT          438 a          475 c          465 g          399 t
ORIGIN      
        1 ccggtgtaag aacgttcata aatgtcctga cacagtgttt ctgtttcgcg acaaagagag
       61 gagcagtacc attccggatc cggcttatga ttttccgggg catgatgtac ttctgtcagc
      121 ccgcaggagg cacagatacg aagctcatcc acgtggtgga atgatacaaa acggcggcct
      181 tctgaaaagg ggtaccagtc accgccgtcc aggcaacgat cgagaaaatc gtcattcgct
      241 tccggtgata aattcaggcg gttccggaag agggttttca gttcatcagg ggtaaatgac
      301 aggctctctc tcagcttttc gttgaaaccc gccatgtttc tttcatacca ggcattgtcc
      361 agtagccagt ttccgtctgc cagttcaaat tccctcagtt ctccccggga aatccgttgt
      421 gaaatgagtg tcagccagat gcgctccgtc agcgtttcag gaaggctgta atcgttttgc
      481 aaactgctca gccacgattt actgttgagc accggttgtt cttccagtgt ctggcggatg
      541 gcactggtca gtcttttcag cgcctggatc tggctggccg cccatagcag cgtcactaat
      601 gcagccagcc agtacaacaa cccgccccac cacatcagtg agggtgaaat atcgaactgc
      661 aatgcgatca atggtgcgta tgcagaacac gccataagga aaaaagcagt ataaaaacag
      721 cagtaatacc cgttaatttc cttgctgata tcttcaggtg tgagcccgct ctgctgaatg
      781 gttcttatct gcacgggaag agccagcaga ccagcggcga gagcggtggt cagaataaat
      841 tttacagagt caaaagtatg agcctccagt gcgatatgtt taccatcaag tccccacatg
      901 aagctgatga tcattccggc aatcgtcaga aatacgcctg ccagccagcg aatatggaat
      961 aaccgggtta tgcgttcagt gatatagcca gccagtaaag ccggaccgaa cagacacagc
     1021 aatatgaacc ctgccagcgc agacgctgtg ctggttaatg ctctgaacaa aaggcgaaga
     1081 atggcaaatc ctgcgatcag cagaatcagc gcgccgacta tctgtaacat agtcactccg
     1141 tgatgaatat catgttattg tgaatgccag tgaatgtggc actgaaacgt tttcccccgt
     1201 ccggatcccg gtcatgatga ccgggaccac agagaggcaa tgcagaatta tcagaaggtc
     1261 acattcagcg tggcctgact gttataactt tcagcgctgc tgccgttgat gctgtgggca
     1321 taaccggcct gaacgcccag ggtgatattt tcccggacac gggcttccag tcctgcctgc
     1381 agaaccagtg aagtgccatt ctgtgagggt gagaacgtca tcccgctgcc tgcggtggag
     1441 gtccccacac gcatatcacc ccgggagctg aaggtgcgga taacggaagg ctgtacccac
     1501 cagttcaccg gcagttcacg cacactgtgt tttgcactgt cacgcagggg ggcacgggat
     1561 gaggtgcctt caccaaaggt catatcgttg tggctgccca gacggaaacc ggcacgcaca
     1621 tgttgtgcac tgccatgccc gaacttcaca taaccggcgt tgtccttacc gtcatccagc
     1681 gaaagcccct gccaggtgta ctgcagccgt ggctccagca tcaggttgtc agtgatactg
     1741 aagggcagac cggtttccag tgagcccagc cagcccc
//
LOCUS       DAOTRK010000312         1777 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.316, whole genome shotgun sequence.
ACCESSION   DAOTRK010000312 DAOTRK010000000
VERSION     DAOTRK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.316"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..527
                     /locus_tag="QIE41_004782"
     CDS             <1..527
                     /locus_tag="QIE41_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDS3533546.1"
                     /translation="GWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVK
                     FGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTF
                     SSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSS
                     AEGYNGQATLNVTF"
     gene            648..>1777
                     /locus_tag="QIE41_004783"
     CDS             648..>1777
                     /locus_tag="QIE41_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533547.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYN"
BASE COUNT          407 a          468 c          473 g          429 t
ORIGIN      
        1 ggggctggct gggttcactg gaaaccggtc tgcccttcag tatcactgac aacctgatgc
       61 tggagccaca actgcagtat acctggcagg gactttccct ggatgacggt aaggacaacg
      121 ccggttatgt gaagttcggg catggcagtg cacaacatgt gcgtgccggt ttccgtctgg
      181 gcagccacaa cgatatgacc tttggcgaag gcacctcatc ccgtgccccc ctgcgtgaca
      241 gtgcaaaaca cagtgtgagt gaattaccgg tgaactggtg ggtacagcct tctgttatcc
      301 gcaccttcag ctcccgggga gatatgcgtg tggggacttc cactgcaggc agcgggatga
      361 cgttctctcc ctcacagaat ggcacatcac tggacctgca ggccggactg gaagcccgtg
      421 tccgggaaaa tatcaccctg ggcgttcagg ccggttatgc ccacagcgtc agcggcagca
      481 gcgctgaagg gtataacggt caggccacac tgaatgtgac cttctgacag aaccatcgcc
      541 tctctgtggt cccggtcatc atgaccggga cccggaccgg cgcaacggat cttcaacgcc
      601 acattcgctg gcattaacaa taacatgata ttcatcacgg agtgactatg ttacagatag
      661 tcggcgcgct gatcctgctg atcgcaggat ttgccattct tcgccttttg ttcagagcat
      721 taatcagcac ggcttctgcg ctggcagggc tcatattgct gtgtctgttc ggcccggcct
      781 tactggctgg ctatatcacc gaacgcataa cccggttgtt ccatattcgc tggctggcag
      841 gcgtatttct gacgattgcc ggaatgatca tcagcttcat gtggggactt gatggtaaac
      901 atatcgcgct ggaggctcac acctttgact ctgtgaaatt tattctgacc accgctctcg
      961 ccggtggtct gctggctgtt cccctgcaga tcaaaaacat tcagcagaac gggatcacac
     1021 cagaagatat cagcaaggaa attaacgggt attactgctg tttttatact gcctttttcc
     1081 ttatggcgtg ttctgcatgc gcaccattga tcgcgttaca gtacgatatt tcaccgtcac
     1141 tgatgtggtg gggcgggttg ttgtactggc tggctgcatt agtgacgctg ctatgggcgg
     1201 ccagccagat ccaggcgctg aaaaaactga cctgtgccat cagccagaca ctggaagaac
     1261 aaccggtgct caacagtaaa tcgtggctga ccagtttgca aaacgattac agccttcctg
     1321 actcactgac ggagcgcatc tggctgacgc tcatttctca acggatttcc cggggagagc
     1381 tgagggaatt tgaactggca gacggaaact ggttactgaa caatgcctgg tatgaaagaa
     1441 acatggcagg gtttaacgaa cagttgaaag agaacctgtc attcacacct gatgaactga
     1501 aaacgctctt ccggaaccgc ctgaatttat caccggaagc gaatgacgat tttctcgatc
     1561 gttgcctgga cggcggtgac tggtatccct tttcagaagg tcgccgtttt gtatcattcc
     1621 atcacgtgga tgagcttcgt atctgtgcct cctgcgggct gacagaagta catcatgccc
     1681 cggaaaatca taagccggat ccggaatggt actgctcctc tctttgtcgc gaaacagaaa
     1741 cactgtgtca ggaaatttat gaacgccctt acaacag
//
LOCUS       DAOTRK010000313         1765 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.317, whole genome shotgun sequence.
ACCESSION   DAOTRK010000313 DAOTRK010000000
VERSION     DAOTRK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.317"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(106..1134)
                     /locus_tag="QIE41_004784"
     CDS             complement(106..1134)
                     /locus_tag="QIE41_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HDS3533548.1"
                     /translation="MDLLAESITEVTVSGKITNTDRVLNIAYGIDRNFLFGAAVSMQS
                     VIMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNGNIASSFYYFMLYYSKK
                     IARKHFSK"
     gene            complement(1160..>1765)
                     /gene="rfaY"
                     /locus_tag="QIE41_004785"
     CDS             complement(1160..>1765)
                     /gene="rfaY"
                     /locus_tag="QIE41_004785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDS3533549.1"
                     /translation="AYNFKTVKVLRNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFF
                     KSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKFFNYASVFIMLIEYVEGVELN
                     DMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKA
                     RDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          561 a          350 c          296 g          558 t
ORIGIN      
        1 tgaaaaacat catttatttt atttcttcgc tcaatatctc ttttaagtga gacaatataa
       61 atgggcaagt tcattaaaaa atctctgggt gattaatctt aataattatt tggaaaaatg
      121 cttacgcgct attttcttac tataatagag catgaaatag taaaatgaag atgcaatatt
      181 accatttctt ttcaaatgtc tggatttcac ttgatactgc ttttcattcg tggctggaat
      241 aaacgccaca tcattccagc aagaagcctg ataagcctca ttaaaatact tctggcaggg
      301 ataattcgtc cagtcgttcc agggcttcgt tacgccaaca taatgaatca gcttagtatc
      361 atcggtaatg gtctttttat aatcttcatc gcttttattt tttaactcat agtcaatgcc
      421 ataaaagcaa tcataatcgc cgctaataaa aatacaatga ccaaagaaca gaatatttaa
      481 aatatcctga tcaaggcatt tatacttcgt atgctgcggg tcacttgcca gcatagcaat
      541 ggctttattc ataaaatcat ttttcgccca tgcttcaagc tgcagatata ctacaccggc
      601 gttgaaataa ttacctggaa gtccctctat agccagacgc ttcgctggtt tttcctgcat
      661 aaaaggcaca tctttaataa cagctgcgaa cttatcgcca tcaaaattaa tatcaagcaa
      721 tccagctaat gagcctttac agatgacatc agcatcgata tataacagat tttcaataac
      781 ttcactcaga tactggaacg caactaaacg gaagaatgtt gcataagacc actgtttcag
      841 gctggggaag atatcaataa aggcactgga gactttataa attcttactt caacgttagc
      901 atttttgctg gtaaaagcgt taacacgttg tagataatct tcatcaatgt agtcggtaaa
      961 gagatgaaac ttaaccgcaa ggtccgggtt atgcataata actgattgca tagatactgc
     1021 cgcaccaaat aaaaagttgc ggtcaatacc gtaagcgata tttaacacac gatcggtgtt
     1081 agtaattttc ccagagacag tgacttcagt aatactctca gctaataaat ccatgttggt
     1141 tccgttttga ctgtgtggtt tacgctttgc cttttaattt tttaataaat ttgcgtagtt
     1201 tggttctgta gattactgaa tagtagccat aatcttttat ttcgttggca atacccaggt
     1261 gacgttccat ggccagacga tcacgagctt tacgctctgc cgtacagctt ttaccggata
     1321 ggtcaattat cctgacacca tctttactaa cgataaagtt accccggtgt gggtcgcccg
     1381 atagcatatt taaggcatgc aatttttcca ttgaagcctt aatttctgct ttaacatttt
     1441 ctggaataat cggcatatca ttgagctcta cgccctcgac atactcgata agcatgataa
     1501 aaacactggc atagttgaaa aatttacgct cagccagaaa ataaaaatca ttaggaaaag
     1561 taagacctgc acttctgaca cgatcggttt caacgatcaa attctgatag tagtcacctt
     1621 ttacaaaaga tttaaaaaaa cgttcattcc ttttcgtttt cggagcaaag actttaaaaa
     1681 catagcgacc atatttcgta tcaatcaacg atactttggt atcatcaata ttacgtaata
     1741 cttttactgt tttaaaatta taggc
//
LOCUS       DAOTRK010000314         1764 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.318, whole genome shotgun sequence.
ACCESSION   DAOTRK010000314 DAOTRK010000000
VERSION     DAOTRK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.318"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1..491
                     /locus_tag="QIE41_004786"
                     /pseudo
     CDS             1..491
                     /locus_tag="QIE41_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            763..1314
                     /gene="papX"
                     /locus_tag="QIE41_004787"
     CDS             763..1314
                     /gene="papX"
                     /locus_tag="QIE41_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PapX"
                     /protein_id="HDS3533550.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGVYGINHSAYMVLTTLFAAENHCLSPSEISQKLQFTRT
                     NITRITDFLEKAGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWDYLT
                     HDEQELFEVINKKLLAHFSDASS"
BASE COUNT          535 a          360 c          415 g          454 t
ORIGIN      
        1 gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa ctcaggccgc
       61 agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatgggcg
      121 aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca
      181 ggctccggaa gacggttgtt gcgcacgtat tcggtgaacg cacgatggcg acgctggggc
      241 gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat ggctggccgc
      301 tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat acgcagcgaa
      361 ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg aagtcgctgt
      421 cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg aacataaaac
      481 actatcaata agttggagtc attacctaca acgtttatcg acctcacacg aacaacgatg
      541 gtttaccgcc ttgcttacat gaagaaaagt ggaaacaggt tattccggtg tcctgatttt
      601 gtgaaccact acaataatcc tttttcacag acttgtcagc agccagcatt tatgttcttt
      661 tacctgcggg aatttatccg tctgcctgtc gggactactc tgtcatacag aaatcaggta
      721 ggaataaatt gtagtggaaa gtcgatgttt accggatgac tgatgcgcgc ttgtacacag
      781 acagtgtgtt tcagtaatat ggagaataat gaaatgaata acacagacac attagaaaaa
      841 ataatcagac accaaaaaaa caaagacccc gcatatccct ttcgggaaca tttgttgatg
      901 cagctctgta ttcgcacaaa taaaagaatg caggataata tatctgaatt tctgggggtg
      961 tatggaataa atcactcagc atatatggtt ctcaccacat tattcgcagc ggagaaccat
     1021 tgtctgtcac cttcagagat aagccagaaa cttcagttta ccagaactaa tattacccgc
     1081 attacagatt ttttagaaaa agccggatat gtaaaaagga cggatagcag ggaggatcgc
     1141 cgtgctaaaa aaatcagtct gacatctgaa ggtatgtttt ttattcagag gctcactctt
     1201 gcacaaagca tgtatctgaa agaaatctgg gattatctga cccatgatga acaggaactg
     1261 tttgaagtca ttaataaaaa gttactggca catttttctg atgccagctc ataaagtgcg
     1321 aaatatctga ggatgctgga tagcttcagg caagagaata atgattcttg cagatgtgtt
     1381 tttccggata caaaaacaaa agtaaaaaat tgcagcgcca ggccaccttt caaagcaggg
     1441 aggcctgtac cgcgtcgaaa atttcagcca gttaatgtaa ttgtctgaac caggcacttc
     1501 gcccgggcag gagaaggagc gtggttgcgg tcatcctgta tactgaagtc gttatgaagg
     1561 aataatacaa ataattgccc gttttctctg tacgatagag gcaggtgtgt atgattgcat
     1621 tactatgttt ttgtgatata aaaaactaaa tggtgagata tgcattttaa aatataacaa
     1681 aaatcaccat gaaataagaa taattatcca cggagaacat aaactgcaga ctgttacaat
     1741 gcatgctgtt catcttgaca atgt
//
LOCUS       DAOTRK010000315         1670 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.319, whole genome shotgun sequence.
ACCESSION   DAOTRK010000315 DAOTRK010000000
VERSION     DAOTRK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.319"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(158..1204)
                     /locus_tag="QIE41_004788"
     CDS             complement(158..1204)
                     /locus_tag="QIE41_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDS3533551.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVILHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEETVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            complement(1206..1484)
                     /locus_tag="QIE41_004789"
     CDS             complement(1206..1484)
                     /locus_tag="QIE41_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533552.1"
                     /translation="MAHDTKLHNSDDSAVFASRHGRRSRTFKSDWFQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERSPRPSRTFQQRIWR"
BASE COUNT          380 a          447 c          459 g          384 t
ORIGIN      
        1 ttaatttcag ccgctgctgg cgaacaataa gcgccacagc ccggcgataa cgctttccct
       61 cctccgagat aaaatatgtg ctgccacggc gtcgccagta agtgttcacc gtcggcgggt
      121 aaggtaaaac caaatctatg agcatcagtc acctctttta cccaagcacg ccagttgcaa
      181 aggcgtgatc aagaaaacga aaaattaaat caacctgaga accatgcttt tcttcgaacg
      241 ccagaggatc cgcatgaagc tcgttgtgat gctcccgaca cagcggtagc gtgaaaatat
      301 cgtgagattt tgtccccatt ccgccctgac catgaccaat caggtgatgg ggatcgtcgg
      361 ctggcttacc acaacacgca cacggctgtg tcttcaccca gcgtgtgtat ttctcgttaa
      421 cccagcggcg acgtttaggt cgtttcatga aagattccgg agactcagga tcaacggcaa
      481 tgctgaccac cgtctcttcc tgtggcgggt tttgctggtg ggcgtgaggc agcggcgcaa
      541 gattttttgt gcgctgcttc agtatgctgg tggcggtctg ctctcccggt acgatgtcgc
      601 tttcacggta cattgagcgg attttttccg cacgcaaccc cagcgaacga cgtaataccg
      661 cttccggtag cgcgtccgcc acctgattgc ggaccgccca ccaggataat tcagccagcg
      721 ataattcccg ttcctgcgag ccattcattg catggagtat gacgtcaatc atccatgctg
      781 acaggttttg atgagcaagt tgctcgagtg attcggatgt ctggtcacgc agctggttgt
      841 cgcagtgcca gcacaacacc attgcgccgg taccataacg gtgaatgacg gtttcgctgt
      901 gatgataatc gccgtgtggc cactggcagg atttaatatg gcgcaacagc cagtcagaca
      961 atgcaccagc accaccagca gcacgaatca cccgtgcgtt actgaaaaac ggcagcaatg
     1021 ttttgtcttc cactagcggc tggcgaacgg caggaacaac cccggacggc agattacgca
     1081 tgcttttcgg ttccggctcc accagtaccc gggtattgtg gaataccggc atggattcac
     1141 ggcccggctt aacgatcacc agcccgagtt ccggtaccag aacaggtcga agtaatactc
     1201 gcacgttacc tccagatgcg ttgctggaat gtgcgggacg gacgcggtga tcgttcggag
     1261 taaggaagcc tgacggagat tatccagtga cgataatcga ggctgagggc tttcttaatc
     1321 tcgtatccgt gtctgcggta gcactgaatt agccactcgg cctgttcttc agtgcatggg
     1381 ggatgctgga accagtcaga tttgaaagtg cgggaacgcc gcccgtgcct gctggcaaag
     1441 acggcagaat catcagaatt gtgtaatttg gtatcgtgcg ccatcggttg tctctgctgg
     1501 cgcagcaggt gccagttgtt caggctggcg tgcgaattgt aaaccagaat gccaggaaaa
     1561 aacaaaaccc gccgaagcgg gttacgtgcg ggtgcgttga ggatgcctga cacatcagag
     1621 gtggcgaggg atttctccct cgccgggtct cttactcctc aggttcgtaa
//
LOCUS       DAOTRK010000316         1633 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.320, whole genome shotgun sequence.
ACCESSION   DAOTRK010000316 DAOTRK010000000
VERSION     DAOTRK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.320"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(321..1457)
                     /locus_tag="QIE41_004790"
                     /pseudo
     CDS             complement(321..1457)
                     /locus_tag="QIE41_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006229500.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          464 a          426 c          285 g          458 t
ORIGIN      
        1 aagtcctctt cactaattaa ccgtaaaatc ccgtaccaat gaaatataaa aataaccacc
       61 cacacaaatt tattgcaaat attatttatt attgtatata catcattcac tgaacgttct
      121 caaactgcat accttagaac catcaattaa agccacaaca aaaaaaatcg attactttat
      181 gtcgcatgac aaatatgaat tacatattaa tcaaccttaa aaacaatcat ctcttataat
      241 taacagcata ccatgagaaa cacaaaatca atttaccatg cataccagga gcataattaa
      301 cctctcaccg cagggcgaaa tcatgaaggc ccgcagccag caaggattag agaaaggtat
      361 tcattcttac gtcctgcccg cttttgcttg cgtttaatac ccgctttaaa gctattctct
      421 gcggtcagta aattcatcgc gatatgtcga caaactgcca gattttctcc cgcttctcca
      481 cgaacaatct ggcattcatc ttcccgcatc cccatatcaa gtcgccagtg cagttgattt
      541 tctaccgacc agtgtgctcg tgttgcttcc aggagttctt tcgccgtaag cgtcaccgaa
      601 cttatgtagt agcgaagaat acacttctct gatttcaccg ctgtttctcc ttcctgtctg
      661 ctcgccagca ctattcctat cgtgctcagc tctgaccatt cataagctaa atcacccaga
      721 gcggttaaat catggctcac aatgcagcat cgtgtatctt cccgtccatg ccctttttcc
      781 tgcgtgctgt aactgtcata cctctcaccc agccacattg tcggcgagta cccgttatca
      841 aacgcctccg ctaattttcc ctgatttcct ttcagcgcca gcaggtaacc ggctttcttt
      901 ttcagaattt cacgggcgat tatatgctgg caacccattg catccagcgt gaccagacac
      961 ccttttaatt ccagcaactg gagccgttcc gggatcgccg ttatctcatt cgattttgcc
     1021 cctgttttca cctgccccaa aaccagacga ttttctgcgc taaaagcact gaccctatgg
     1081 atcacggcat tacgtttcga tttatcgtaa gtaccccgta atgacttacc atcaatcgcc
     1141 acaacagagc cttcagacaa cgtgtgacag tccttcatct actggataaa aaggggctgt
     1201 aattgttttg ctgaaatcat tcccatcact cgggcaatcg tgtgatggga gggggtgcca
     1261 ttttcaaaat caccatacag gcgtaaccat tccagatgct cctcaccaaa atcagcaatt
     1321 tcttcccatc cttcagcgcc accgataatg gcggtgatcg tcagaaaaag gatatccgcc
     1381 aggctgtaac tggttttcca ggactggcga taatcgtgaa taattgaaat ttgatccaaa
     1441 aggcttagtc cgttcatggt gtgctatcca tcaaaaagaa aggataagat cacactgaaa
     1501 taagatggtc aaattgatcg aaaaaaaaga tcaacatgat gggttttacg ataaaagtaa
     1561 tgtcgcgatt taaaaattca tcattcacca aagtcttgtg gggcgcgggc tttcatgctt
     1621 tttccctgat acg
//
LOCUS       DAOTRK010000317         1631 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.321, whole genome shotgun sequence.
ACCESSION   DAOTRK010000317 DAOTRK010000000
VERSION     DAOTRK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.321"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1631
                     /gene="eilA"
                     /locus_tag="QIE41_004791"
     CDS             <1..>1631
                     /gene="eilA"
                     /locus_tag="QIE41_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HDS3533553.1"
                     /translation="KNNGILLFKNKEYHIPPKELGVIILLLNADGEIVSKEEIIDKVW
                     SASVASDESLTRCIYALRKLLHENKQCKYIETVYGRGYRFTVPIVIVTDNEPVKSTTT
                     LAVFPFRTEGSINVLKLHYELVQGLSKYAFCGLDILPASVTNEASDFSTIHQFINKTG
                     PEYYIMGQVVHYGKNWRLFIELIHARTHKLIDHQSIDFNPDNPLSVLLSQVINILIEK
                     IPNINFKSINTQQMPSLDSAVMYMNGRMEMYRYTPESLKRALVIFRDCVSTQPQNTLP
                     YCCLAECHISLALLGLSEQKQAITTAVTSIEKALEINPSNSQALGLLGLISGLKDEHS
                     VSNVLFKQAHLLKPNSPDVYYYQSLLCFLNGDLARAFNLIEKSIALEPNKMGISILKL
                     IILYYTSPLDNAISFALNLNSQNTCNNPIITSILAMFMALKGHNDKAKSLLLKLEPEH
                     GLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKINGVILPLIYTVYGKKEYEKRW
                     QQLIKDNDLWSNVLLHDPRLLSVKNEFNTIGVMRT"
BASE COUNT          530 a          297 c          280 g          524 t
ORIGIN      
        1 aaaaaacaat ggaatattat tattcaaaaa caaagaatat catattcccc cgaaagaact
       61 tggcgttatt attctattgc ttaatgctga cggtgaaatt gttagcaaag aagagattat
      121 cgataaggtc tggagcgcga gtgttgccag tgatgaatct ctaacacgat gtatttatgc
      181 attgcgcaaa cttttgcatg agaataaaca gtgtaagtat atcgaaacgg tctatggtcg
      241 tggataccgt tttactgtac ctattgtgat tgtgaccgat aatgaaccgg taaagtcgac
      301 cactactctt gccgtttttc cctttcgtac tgaaggttcg atcaatgttc taaaactcca
      361 ttacgaactt gtccagggcc tttcaaaata tgctttttgt ggtttagata ttttacccgc
      421 ttcggtaaca aatgaggcca gtgatttttc cactatccat cagtttatca ataaaacagg
      481 tccagaatat tatattatgg gtcaggtcgt tcactatggt aaaaactggc gtttatttat
      541 tgagttaatt catgccagaa cacataaatt gattgatcat caaagtattg atttcaaccc
      601 cgataatcct ctttccgtat tattatcaca agtaattaat attcttattg aaaaaatacc
      661 caatattaat tttaaatcta ttaatacaca acaaatgcca tctctggatt ctgctgttat
      721 gtatatgaat ggcagaatgg aaatgtatcg ttatacacca gagagtttga aacgagcatt
      781 agtaatattt agagattgtg tgagtacgca accgcaaaat accttgccct actgttgcct
      841 ggcggaatgc catatatcat tagccctcct tggactcagt gaacaaaaac aagcaataac
      901 tacggcagta acctccattg aaaaggctct tgaaattaac ccttcaaatt ctcaggcatt
      961 gggtttactg ggtttaatca gtgggctaaa agatgaacat tcagtgtcta atgtgctatt
     1021 taagcaagca catttactca aacctaattc gccagacgtt tattattatc agtctctgct
     1081 atgttttttg aacggtgacc ttgcaagagc atttaatcta atagaaaaaa gtattgctct
     1141 tgaacccaat aaaatgggta taagcattct taaattaatt atactttatt acacatcccc
     1201 cctcgataac gcgatatctt ttgcattaaa tcttaacagt caaaacacct gtaataatcc
     1261 aattattacc tctattctgg ctatgttcat ggcactgaaa ggacataatg acaaagccaa
     1321 aagtttattg cttaaacttg agcctgaaca tggtcttgat tacacctgtg ttaactcgct
     1381 ttatacgaaa tttctgatct atggcgcatc tataaaaaat gacattatga agttactggc
     1441 aaatattaac accaacaaaa tcaatggtgt gattttgccg ttgatttaca ctgtgtatgg
     1501 taaaaaagaa tatgaaaagc gctggcaaca attaattaaa gacaatgatt tatggtcaaa
     1561 tgttttgctt catgaccccc gtttgctcag tgtaaaaaat gaatttaata ctatcggtgt
     1621 gatgcgtact t
//
LOCUS       DAOTRK010000318         1630 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.322, whole genome shotgun sequence.
ACCESSION   DAOTRK010000318 DAOTRK010000000
VERSION     DAOTRK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.322"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            498..817
                     /locus_tag="QIE41_004792"
                     /pseudo
     CDS             498..817
                     /locus_tag="QIE41_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(801..935)
                     /locus_tag="QIE41_004793"
     CDS             complement(801..935)
                     /locus_tag="QIE41_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDS3533554.1"
                     /translation="MKIVQTSLSESVTQHLAERILCGELPAGSVLPGENELATVSRGG
                     "
     gene            complement(1155..>1630)
                     /locus_tag="QIE41_004794"
     CDS             complement(1155..>1630)
                     /locus_tag="QIE41_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDS3533555.1"
                     /translation="AGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVR
                     GQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWV
                     PTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVASIT"
BASE COUNT          447 a          396 c          370 g          417 t
ORIGIN      
        1 gctaccacag tgacagtcac tgctggaaaa tatacctctg ttcaaaacag tcctgttgtg
       61 gatccgaaaa atacacatcg ctcccgttat ctgtggctgt atgattacca tcactgagta
      121 aaaaaaatcc ccaggatgca ggggacggaa ggtaaactat gctgaatctt gtctgtttgt
      181 tttaatattt atctatccgt cttgccaacg gacaaagaaa aaataacacg acgcagaaca
      241 aaagaaaata ttattttcat atcttccgga agactatggc ctcttccaga taattacctt
      301 atggttggca tcaatcaatg attcactctc gttctgcctc ggaactctat atggatgtac
      361 aatcatataa tgttctaata ttcacgagat gtgaataaac aaatccactt aagaaacggg
      421 ggggtattta attaataact aagtgaagcg ccccggaaat cttggagact aaattccctg
      481 agaaagaggt aaacaggatg gctaaaaata ctcgtttttc ccccgaagtc cgtcaacgga
      541 cagttcgtat ggttctggaa aatcagggcg aatatgactc ccaatgggca gcaatttgtt
      601 ccatcgtccc aaagattggc tgtacaccgg agactctgcg tgtctggtgt acaaccagca
      661 tgagtgggat accgggggcg gtgatggtgg actcaccacc gctgaacgtc agcgtctgaa
      721 agacgctgga gcgtgaaaat cgtgaactgc gccacagtaa cgatatcctt cgccaagctc
      781 ttattttgcg aaggcggagt tcaaccaccc ctggaaactg ttgccagttc attttcgcca
      841 ggcagcacgc tacctgctgg cagttcgccg cacaggatgc gctcggcaag atgctgagta
      901 acagattcgg agagactagt ctgcacaatt ttcattgcat ttcctagcta aaaaacgtca
      961 cagtgatgag ccatataata agctaaataa cagcaaactt actaattgtg aagcaggtga
     1021 actccacccc ctagtcagtt cttatcgttg ctggataccc tcaaagcagt gtaatgctat
     1081 ccagaatagg catgcttaag gaagagctca agggtagaac cccgacttcg gcatcgcaga
     1141 ggatactgta gtggtcaagt aatactggcc acggttttac agtaaaaatg atatctgttc
     1201 tctgactctt ccggcgtcag ccctccgtta taatggtgag gcctgacgct attgtaataa
     1261 ttcagaatat aactgctgat ttgctgccgg gccacgtctt tgcctgtgta gccatcggtt
     1321 ggcacccatt ctgttttcag actgcggaag aagcgttcca ttggactgtt atcccagcag
     1381 tttccccgtc ggctgacact ttgctttatc ctgtaacgcc agagaagttg ttgatatttc
     1441 agtcctgtat attgacttcc ctggtcgcta tggaacatga cgtcccgcgg ctgaccacgc
     1501 acctcatacg ccatccgcag ggcactgctt atcagggcag tatcggcatt cgctgacagg
     1561 ctccagccga taaccctgcg ggcaaaaaga tccatgacga ccgccagata gcaccagcga
     1621 tttcctgccc
//
LOCUS       DAOTRK010000319         1630 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.323, whole genome shotgun sequence.
ACCESSION   DAOTRK010000319 DAOTRK010000000
VERSION     DAOTRK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.323"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            90..500
                     /gene="mscL"
                     /locus_tag="QIE41_004795"
     CDS             90..500
                     /gene="mscL"
                     /locus_tag="QIE41_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDS3533556.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(497..715)
                     /gene="arfA"
                     /locus_tag="QIE41_004796"
     CDS             complement(497..715)
                     /gene="arfA"
                     /locus_tag="QIE41_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDS3533557.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(771..1196)
                     /gene="zntR"
                     /locus_tag="QIE41_004797"
     CDS             complement(771..1196)
                     /gene="zntR"
                     /locus_tag="QIE41_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDS3533558.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESA
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            complement(1207..1575)
                     /locus_tag="QIE41_004798"
     CDS             complement(1207..1575)
                     /locus_tag="QIE41_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDS3533559.1"
                     /translation="MWLLDQWAERHITEAQSKGEFDNLPGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYKEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
BASE COUNT          429 a          393 c          350 g          458 t
ORIGIN      
        1 ttatcaagga ttaattaaat ccattcctgg caggaaaata gcttaacatt tgttagactt
       61 atgattgtcg gcttcatagg gagaataaca tgagcattat taaagaattt cgcgaatttg
      121 cgatgcgcgg gaacgtggtg gatttggcgg tgggtgtcat tatcggtgcg gcattcggga
      181 agattgtatc ttcactggtt gccgatatca ttatgccacc tctgggctta ttaattggcg
      241 ggatcgattt caaacagttt gctgtcacgc tacgcgatgc gcagggggat atccctgctg
      301 ttgtgatgca ttacggtgtc ttcattcaaa acgtctttga ttttctgatt gtagcctttg
      361 ccatctttat ggcgattaag ctaatcaaca aactgaatcg gaaaaaagaa gaaccagcag
      421 ccgcgcctgc accaactaaa gaagaagtat tactgacaga aattcgtgat ttgctgaaag
      481 agcagaataa ccgctcttaa caagcgcctg aaagcagaag gccagtggta aaaaagtgat
      541 ttactttctt gccactggcc tcccagttcc cccgattgcc atgttttcct tttcgcatgt
      601 aactgccttt ccctttctta tttctctcta cgcgctgtcg gaataaggga tcatgtagta
      661 atgcttctat cgcattatcc tttatctgcc ctttagtatg ctgatatcgg ctcataaaaa
      721 ctccaattgg ttatttaacg gcgcgagtgt aatcctgtca gtgcaaaaaa tcaacagcca
      781 ctcttaacgc cactcgcccc ttgttcaaga gcttcaagaa tcgagcaata aacactgcta
      841 tgggcggtcc cacaacaggc atcgttaagg cgttgcaagg aacgctgcat actctgcaac
      901 tcggctatcc gtgcttcgac ttcctgcaat ctttcctgca caatgccttt tgactcctga
      961 caagtatggt gttcaggatc gatacggatc gacagcaact cgcggatcga ctccagacta
     1021 aaaccaagtt gtctggcatg gcggataaat ttcaatcgct ggagagcgct ttcggtatac
     1081 aggcgaaacc caccttcggt gcgcacttca tgctccatca tctgctgctt ttcgtaataa
     1141 cgaatcgtgt cgggtgttac ttctgccatt tttgccagct caccaatgcg atacatacat
     1201 actccactag ttatcgttga ttttgtccaa caacttgtca gcataatcgc cgcgtaaaaa
     1261 atcggtactc aatccagctt gtcgcagctt caattccagt aatgacaatc gacgactaac
     1321 ctctttatat tgcggatcat catgacggat acctttgagg atatccagaa gctgtattgc
     1381 ttccctccgc tgctcaagtt ctggcggtaa gcaaccggca ttcttcagca agcgataccc
     1441 cgcacgtaat tccggtggca cgtgtgaatc atcatccaga atcaatggct cgccacttcc
     1501 cggaagatta tcaaactcac ctttggattg tgcctcagta atatgacgct ctgcccactg
     1561 gtcaagtaac cacataaata ctccagggga tgaacaaaaa gagtacagct attgtagata
     1621 agtggggata
//
LOCUS       DAOTRK010000320         1590 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.324, whole genome shotgun sequence.
ACCESSION   DAOTRK010000320 DAOTRK010000000
VERSION     DAOTRK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.324"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(158..343)
                     /gene="rzoD"
                     /locus_tag="QIE41_004799"
     CDS             complement(158..343)
                     /gene="rzoD"
                     /locus_tag="QIE41_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDS3533560.1"
                     /translation="MRKLKMMFCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSGNDW"
     gene            complement(560..1057)
                     /gene="rrrD"
                     /locus_tag="QIE41_004800"
     CDS             complement(560..1057)
                     /gene="rrrD"
                     /locus_tag="QIE41_004800"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDS3533561.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(1057..1272)
                     /gene="essD"
                     /locus_tag="QIE41_004801"
     CDS             complement(1057..1272)
                     /gene="essD"
                     /locus_tag="QIE41_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDS3533562.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDRVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          400 a          404 c          355 g          431 t
ORIGIN      
        1 attatgatta tactgtataa aatttaactc ctcttagatc ttacttcact gttccttatg
       61 aaacaatcat caaaatgaat catattgtag ttaagatttt actttaaaca ctgctcggtt
      121 atgtattgct gagcaccttc aagttgggcc tgcatcatta ccagtcgttc ccggagggtg
      181 aaataatccc gttcagcggt gtctgccagt cggggggagg ctgcattatc cacgccggag
      241 gcggtggtgg cttcacgcac tgactgacag actgctttga tgtgcaaccg acgacgacca
      301 gcggcaacat catcacgcag aacatcattt tcagctttcg catcagctaa ctccttcgtg
      361 tattttgcat cgagcgcagc aacatcacgc tgacgcatct gcatgtcagt aattgccgcg
      421 ttcgccagct tcagttctct ggcatttttg tcgcgctgtt ctttgtaggc gatggcgtta
      481 tcacggtaat gattaacagc ccatgacagg cagacgacga tgcagataac cagagcggag
      541 ataatcgcgg ttactctgtt cattgctgac cccacaaaca gatttcacgc tcaatctcac
      601 gacgagtcat gagacctttc cattgcttac cgccagcata tgtccagcga cgtagctgat
      661 cacatgcgcc tttgatatcg ccctggttta ttttgcgaag aagcgtcgat gttctgaaat
      721 tgccagcacc cacgttgtaa acgaatgagt aaagagcgcc gcgcgttgtt tccggtatat
      781 cgactttgat gtacgggtta atttgtctgg cgaccgtggc aaggtcttta ttcaggaggg
      841 ctttgcattc tgcttcggta tacgttttac cgggaatgat gtcttttccg gtgtgtccgt
      901 gacatacagt ccatacacca acgatatctt tatatggtat gtagctgaca ccttccagac
      961 catcgttacc acttgggcca gtgattaaca ctgatgctat agcaattgct ccgccaccaa
     1021 tagcagcagc aacggctttt cgtaatgatg gaggcattat tcacctctcg cagccttgcg
     1081 cttatcttct ttaatcttga aataaaggtt tgtcaggtac gtcagcaggc caaataccag
     1141 actacccagc acaccgattg cagcccactg tgacggagtt actctatcga gcaactgtaa
     1201 aaaccagtag ccagcactgc ctgcggaggt gccataggcg acacccgttg ttaacttatc
     1261 catggatttc ataaccccca cctcgcagat gcgggcgctg tgtaatggaa acaaaaaatg
     1321 gccaccagcg gcccgtaaaa aacaccccgt caaaagcacc ggcatccgca gatgcccttt
     1381 gcgtggcgtt atttgatgcg cgccagatgt ggcgcaaaga aatgaaataa gacttatcga
     1441 aaattaaggt caatttgatg atttaaacca cttctgaagc ttagtagtat gaacatgtcc
     1501 ccagaagggg gccaatactt attattcttc atggactttg tcccgcggtc ttaatccgac
     1561 gaccgcgcta ctttttcacc ctctcgcaaa
//
LOCUS       DAOTRK010000321         1587 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.325, whole genome shotgun sequence.
ACCESSION   DAOTRK010000321 DAOTRK010000000
VERSION     DAOTRK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.325"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..708
                     /gene="cpoB"
                     /locus_tag="QIE41_004802"
     CDS             <1..708
                     /gene="cpoB"
                     /locus_tag="QIE41_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDS3533563.1"
                     /translation="PISSVGSGSVEDRVTQLERISNAHSQLLTQLQQQLSDNQSDIDS
                     LRGQIQENQYQLNQVVERQKQILLQIDSLSSGGAAAQSTSGDQSGAAASTTPTADAGT
                     ANAGAPVKSGDANTDYNAAIALVQDKSRQDDAMVAFQNFIKNYPDSTYLPNANYWLGQ
                     LNYNKGKKDDAAYYFASVVKNYPKSPKAADAMFKVGVIMQDKGDTAKAKAVYQQVISK
                     YPGTDGAKQAQKRLNAM"
     gene            873..948
                     /locus_tag="QIE41_004803"
     tRNA            873..948
                     /locus_tag="QIE41_004803"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:906..908,aa:Lys,seq:ttt)
     gene            984..1059
                     /locus_tag="QIE41_004804"
     tRNA            984..1059
                     /locus_tag="QIE41_004804"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1017..1019,aa:Val,seq:tac)
     gene            1062..1137
                     /locus_tag="QIE41_004805"
     tRNA            1062..1137
                     /locus_tag="QIE41_004805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Lys,seq:ttt)
     gene            1189..1264
                     /locus_tag="QIE41_004806"
     tRNA            1189..1264
                     /locus_tag="QIE41_004806"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Val,seq:tac)
     gene            1268..1343
                     /locus_tag="QIE41_004807"
     tRNA            1268..1343
                     /locus_tag="QIE41_004807"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Lys,seq:ttt)
     gene            1490..1565
                     /locus_tag="QIE41_004808"
     tRNA            1490..1565
                     /locus_tag="QIE41_004808"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1523..1525,aa:Lys,seq:ttt)
BASE COUNT          434 a          381 c          401 g          371 t
ORIGIN      
        1 ccaatcagta gtgtcggctc aggctcggtc gaagaccgcg tcactcaact tgagcgtatt
       61 tcaaatgctc acagccagct tttaacccaa ctccagcaac aactctctga taatcaatcc
      121 gatattgatt ccctgcgtgg tcagattcag gaaaatcagt atcaactgaa tcaggtcgtg
      181 gagcggcaaa agcagatcct gttgcagatc gatagcctca gcagcggtgg tgcagcggcg
      241 caatcaacca gcggcgatca aagcggtgcg gcggcatcaa cgacgccgac agctgatgct
      301 ggtactgcga atgctggcgc gccggtgaaa agcggtgatg caaacacgga ttacaatgca
      361 gctattgcgc tggtgcagga taaatcccgc caggatgacg caatggtggc atttcagaat
      421 ttcatcaaaa attaccctga ttcaacttac ctgccaaacg ccaattattg gctgggtcag
      481 ttaaactaca acaagggtaa aaaagatgat gcggcgtatt attttgcttc ggtagtgaaa
      541 aactatccga agtcaccaaa ggctgcagat gcgatgttta aagtcggcgt catcatgcag
      601 gacaaaggtg ataccgcaaa agcgaaagcc gtataccagc aggttatcag taaataccct
      661 ggtactgatg gcgctaaaca ggcacaaaaa cgtcttaacg cgatgtaatg cataacacac
      721 gaccagaagt cgcattattt ctggtcgtgt cgtgcgaatc ataagcagtt gagtgatcta
      781 catcgaaatt tttgttgcgc tcaagtctga aatcagtaat atatgccgcc gttgccacgg
      841 gatatcaaac aaaccgaaag caacgaaaaa gtgggtcgtt agctcagttg gtagagcagt
      901 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccact aacctagtta
      961 gttgtagtat ccagcgtagt atcgggtgat tagctcagct gggagagcac ctcccttaca
     1021 aggagggggt cggcggttcg atcccgtcat cacccaccac cgggtcgtta gctcagttgg
     1081 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccacta
     1141 attacggtgg gttcggtgga agtagtttgt agtatccagc gcagtatcgg gtgattagct
     1201 cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc
     1261 accactcggg tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg
     1321 ttcgaatcct gcacgaccca ccaattttaa catcaaactc agatgttaag cgtgaaggat
     1381 aacgttgcgc cagcaacggc ccgaagggcg agcgaagcga gtcatcctgc acgacccacc
     1441 aattttaaag attggttccg agtagaaatc tttcaggtaa cacccgtatg ggtcgttagc
     1501 tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc
     1561 caccatcctg aatgattaaa gcagcat
//
LOCUS       DAOTRK010000322         1571 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.326, whole genome shotgun sequence.
ACCESSION   DAOTRK010000322 DAOTRK010000000
VERSION     DAOTRK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.326"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..184
                     /locus_tag="QIE41_004809"
     CDS             <1..184
                     /locus_tag="QIE41_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533564.1"
                     /translation="QNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLG
                     NMVNRLEEALPPELPA"
     gene            356..714
                     /locus_tag="QIE41_004810"
                     /pseudo
     CDS             356..714
                     /locus_tag="QIE41_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            793..1026
                     /locus_tag="QIE41_004811"
     CDS             793..1026
                     /locus_tag="QIE41_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDS3533565.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            1126..>1571
                     /locus_tag="QIE41_004812"
     CDS             1126..>1571
                     /locus_tag="QIE41_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDS3533566.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRV"
BASE COUNT          402 a          415 c          389 g          365 t
ORIGIN      
        1 tcagaatgct ctggagatca ttttcgccgc cggtatacaa caacgggccg cgaccaatat
       61 gttaatcaaa ccactggtgg tcagtatcat caggcaacgc cccgtcatgg aatatgatgc
      121 atcccatctc ggcaatatgg ttaaccgact ggaagaagca ttacccccgg agttaccggc
      181 ataacggcta ttctgcgcct tttacgcttt atcacctgat ttgtcagtgt catcgtcatg
      241 actcagagag gtaaatacca tgattcacct gttcaaaacc tgcatgatta ccgccttcat
      301 tctggggtta acgtggtctg ccccactccg ggcacaggat caacgttaca tcagtatacg
      361 caatacagat acgatatggc tcccgggaaa tatttgtgct taccagttcc ggctggataa
      421 tggcggaaac gatgaaggat ttggccccct caccatcact ctgcaactca aagacaaata
      481 tggtcagacg ctggtgacca gaaaaatgga aacggaagct tttggtgaca gtaatgccac
      541 gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa aatgtcgcca caaccgaaat
      601 cattaaagca actgaagaaa gtaacggcca tcgtgtcagt tgccgttatc ggttttcaat
      661 ccccaggact accatccact gctgattacc gtttccggaa aaaacgttaa ctgatccctg
      721 aagtcagttt catgcttcag cctctgtcac aacgcgtggc agaggctttt tatttatcag
      781 gagaaaacag atatgccggg ttacacagag tatgtcctgg ctgaaggatc tttctcatac
      841 gggcaggcgg ttgctgtcat aaccgcttac cgcaatgtgt ttatccagga tgacccggga
      901 atgcatttcc gtcgggttat ccgcaacgcg gaaggtcagc gacgctggcg ctgccggaat
      961 tctgaacccg atgccggaaa ggtgcttaat acgcggctcg ccagtgacgg tcttctcaga
     1021 caataaacgt cattcatttc acccatcagg ccgcgtcttc tccgggagac gtggcttttt
     1081 catttattac accagtaaat cttaaccacc gataaggagc aaattatgcg actagccagt
     1141 cgttttggtt atgcgaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
     1201 cactatgtgc cggggatttt cggggaagat aaacacacgt cccgaagcca gaactatacg
     1261 tacatcccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1321 gcctgccaga cccgtgtgcg cgacccgggc cgccggggat acacaaaaca catgctgcgt
     1381 ctgcggcggg ccggagagat aaacggagaa catgtccctg aaattattct gctcaactct
     1441 catgacggta cctccagcta ccagatgctg ccggggtact tcaggttcgt ctgccagaac
     1501 ggctgcgtct gtggtcagtc tctgggggaa gtacgtgttc cgcaccgggg aaatgtggtg
     1561 gacagagtca t
//
LOCUS       DAOTRK010000323         1552 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.327, whole genome shotgun sequence.
ACCESSION   DAOTRK010000323 DAOTRK010000000
VERSION     DAOTRK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.327"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(924..1400)
                     /locus_tag="QIE41_004813"
     CDS             complement(924..1400)
                     /locus_tag="QIE41_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3533567.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          345 a          412 c          393 g          402 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctcctgcacg ttttttgccg tccgttcacc gttaaccccc
      241 gcagaaatcg ggacaggatg tgcaaatgta ataccgtcac ccgcgacgat attccgtttg
      301 ccatctggtc agggcttcgc cccgacaccc cgtatcagtg cgtacgtgct caatgtggtt
      361 tccggttctc ttccgcttcc tcgcccactc gctcactaac gttcggtcgc tggggctgcg
      421 gcgagcggtg ccacctctcg catgttgtcg gtgattccgc tgttgttctt tcggggatga
      481 gtcgtcagat agcccttcaa acgcgctcag aagctctcag acgcacgagg cggggtttaa
      541 atgcccgtca gggcatggaa ggcgcgaaaa acgcttacag agcgtttcag gaggtcaggc
      601 gcgccgatac ccctagccag gggctttcag gtcgccagga accgcgccag ccccctgcaa
      661 agcccatttg cacggtacag gcagtcactc ggttggtagg tggtcgtaga ccgtcggtgt
      721 tacagcgatg ggggtctggg gttctcccca gacggtttcc ggttgcgctc aaggcgctgc
      781 cggaaccgtg gcggctcggt cgttctggag atcggggagc aaaaacacgc aagggatttt
      841 gaggcggcaa aagagcaatg caggatagac aatcatggtc agaacgttaa cacgtagccc
      901 ctgattgtcc gcctgccgct ggctcaattt gaaaacatgg ccatgattcg gcgctgaata
      961 tcaccgtctt tgttgaccag tgttttagtg acaaatgctg catggttttt cgtagtgttg
     1021 tatcccttcc agacttcatg tggatcgagg gcatacgcac aaaccttacc agcaacgggg
     1081 attctaacta atgcattttt tgattctaag taattgagcc aacgaataat tgtgcttctg
     1141 ctaactccaa gtttttcagc taggaattgc tgatccgcaa cgacagcgcc acacgtagga
     1201 tcgatatgct ccgctaaaaa tgagtacagc ttcagtgctg atatgccttg gctatccttc
     1261 agcagctctc gaacgcgttc ccaacctttt ggggatacct gtgtaaacct tgaattcttc
     1321 tttgcttctt cgagatcctc ggtgtttttc tttgcctcat ctgaaagttg tttcagtcgt
     1381 ttttctgaat caataaacat gattttttcc ctctaaaaat cccatgcact aacgttggtt
     1441 caaatctaag ccatcatgac acatctgtgc aacaccttgt tgcgtgttgt cgcatccagg
     1501 atgcgacaac gttgtgtcac cctggatgcg acaggtaaac aaaaaacaca ct
//
LOCUS       DAOTRK010000324         1543 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.328, whole genome shotgun sequence.
ACCESSION   DAOTRK010000324 DAOTRK010000000
VERSION     DAOTRK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.328"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            287..520
                     /gene="papI"
                     /locus_tag="QIE41_004814"
     CDS             287..520
                     /gene="papI"
                     /locus_tag="QIE41_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HDS3533568.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQNCSSTT"
     gene            complement(1171..>1543)
                     /locus_tag="QIE41_004815"
     CDS             complement(1171..>1543)
                     /locus_tag="QIE41_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDS3533569.1"
                     /translation="GFVYVAFIIDVFAGYIVGWWVSSSMETTFVLDALEQALWARRPS
                     GTIHHSDKGSQYVSLAYMERLKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKS
                     WKNRAEVELATLTWVDWYNNR"
BASE COUNT          465 a          367 c          307 g          404 t
ORIGIN      
        1 acaaaacaaa attaaaccag aataaaattc taacaacaag aatacactca gataaaaaca
       61 tcatggtgaa ttgaatttac agcataaaag atcgtctaaa tgcattttta cggactttct
      121 tttcacagaa aaataacaca caaaatgtga tacacatcaa ataaagccaa caaaaagcaa
      181 attcactaaa ttgcacaaat aaagcaaaca cagagaataa aaataccttt ccgttgataa
      241 cagagagcat gatttttatt attcgaagaa actttgttac agtgagatga gtgaatatat
      301 gaagaatgaa atactggaat ttctgaacag gcataatgga gggaaaaccg cagaaattgc
      361 ggaggcgctg gcggtaacgg attatcaggc acgctattac ctgttattac tggaaaaaga
      421 aggtgtggtt cagcgctctc cattaaggcg aggcatggca acatactggc ttctgaaagg
      481 agagaagcag gcggggcaga actgttcttc cacaacttaa taactcaatc tgtgggcatg
      541 cttcactgaa acagataaaa gttacatcag agtgcagatt caattttgca gcattcatca
      601 caaaatagaa atttagtatt ttatgtcaga atacttcaca tgataataaa aacctgtctg
      661 gatttactgc atggccagac attacaaaat acaaacgcca taaacttcat tcagccttct
      721 tgctgtccgg taacggaggc tgctttttcg ttacaccttt ccccggcagc atcagtagca
      781 gtaatttata gattctacga actgagactg tctgtccggg gagaacgtac acagcccgac
      841 acaatgtacc agtcaataat atccggtttc tgattccctg cctgacagac cactctctgg
      901 caactttgta ttcagttcat ctactactgg agacagattt ataaaatcca actccacgat
      961 ctttgaccgt ccgcatcccc tactctctgt gctgcggtca tcggttaatt ccagcgtccg
     1021 tccgttacga tcagatatta ctgaacctcc ccgggtttcc tggagagtgt tttatctgtg
     1081 aactcaggct gccagatcat tgtttccgat ggaagcataa taagcttttt ctgcttctgc
     1141 cggaggagta tggcccagcc ttcccagcaa tcatcgattg ttataccagt ccacccacgt
     1201 gagtgtggcc agttccactt ctgcacggtt tttccagctc ttacggtgta ttacctccgc
     1261 tttgtaaaga ccattgatgc tctccgccat cgcgttgtca tacgagtcgc ctgtactccc
     1321 tgttgatgcc agcagttttg cttcttttaa tcgctccata taggccagtg acacatactg
     1381 agagccttta tcgctgtgat ggatggtgcc ggacggacgg cgggcccaca acgcctgctc
     1441 cagcgcatcc agcacgaatg tcgtttccat agacgatgag acccaccacc ccacgatgta
     1501 tccggcaaac acatcaatga tgaacgccac atagacgaag ccc
//
LOCUS       DAOTRK010000325         1497 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.329, whole genome shotgun sequence.
ACCESSION   DAOTRK010000325 DAOTRK010000000
VERSION     DAOTRK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.329"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1298)
                     /locus_tag="QIE41_004816"
     CDS             complement(join(<1..818,818..1298))
                     /locus_tag="QIE41_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HDS3533570.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFVVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSR"
BASE COUNT          384 a          354 c          299 g          460 t
ORIGIN      
        1 tctgctgttg tagtattcaa tatattccgt aacagcatcc ttcagttcgc ttatattact
       61 gaactcatca agataaaaac actccgactt taaggttcca aagaaacact ccaccacagc
      121 attatccaga caattgcctt ttctggacat gctttgttta ataccatgtt ctttaaggat
      181 attttgatat cttctcatac gatactgcca tccctggtca gagtgcagaa caggatgctc
      241 gtgaggatta agctttttga atgcctgatc gagcatattc tcaaccatgt tcatcactgg
      301 tctttccgaa aggctgtaag aaataacttc gttgttgaag agatctatta ctggagacaa
      361 atacagcttg cgcccattga ctacaaattc agtaacatcg gtaacccact tctcgtttgg
      421 ccgcgtagcc ttgaaatctc tttggagaac attaggggcg gtttgcccta cctctcctct
      481 gtaagagcgg tatcgcttga ccttaatcgc tgctttaagt gagagggttc ccatcaggcg
      541 ctgaacagct ttatggttaa tctgtttccc ttctcgatga agagacagcg ttaccctacg
      601 gtatccgtat cggcctctat tctcgtgata aatctcacca atacgctttt taacgtccgc
      661 atacttgtca ggcttgctga gagcctttag atggtaataa aacgtactgc gcggtatctc
      721 cgcagccctg agaagctcat caagaggata aaactgcctt agctcgttga gtacttttac
      781 tttttcgtgg gatgagctaa ggctttcagc ttttttagat acataagccg cgtttcaaga
      841 aatcgaactt gcctttcaag atcctcaatg cgtcggtctt ttgacagctc caatgctgat
      901 gccgcttttt ctggatcaac tgatattgca atgtttcttt tggtgccaat cttgagcgcg
      961 cgtaaaccag cttctccgcg ctcttcatag accttcagcc acctggctac agaaccacta
     1021 ccagcaagca taaagtgagc agcagcctga ttaagggaca tgtgctgctc gatcacagct
     1081 ttcacgacct taatacgcaa ctctggatca gcactaacgc ctttaggttt gggaattaaa
     1141 cctttttctc catgtttttc atagagggca acccatgtcc tgacctgggt tcgggggaca
     1201 ccaaaacgtg ccgagatgat cctgtaacca tcatcagttg tgaagtagtg attcacgact
     1261 tcaaggcgct tttcaaaagg gtattttggc tttgacatat taggggctat tccatttcat
     1321 cgtccaacaa aatgggtgca gtacactttg atttatagtc aggtggggct tttctctgtc
     1381 tgcctttcgg tgaatacctg agacaaacag tctcaagcac ccgtggctat tctagcttaa
     1441 taagttcgtt tcttctcctt gatataatcc taaaaaaatc tcataaaatt aatatat
//
LOCUS       DAOTRK010000326         1487 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.330, whole genome shotgun sequence.
ACCESSION   DAOTRK010000326 DAOTRK010000000
VERSION     DAOTRK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.330"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..403
                     /locus_tag="QIE41_004817"
     CDS             <1..403
                     /locus_tag="QIE41_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Transcriptional activator for the hut, put and ure
                     operons and repressor for the gdh and gltB operons in
                     response to nitrogen limitation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HDS3533571.1"
                     /translation="DCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVI
                     GEIESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARA
                     NLSPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            505..1455
                     /gene="cbl"
                     /locus_tag="QIE41_004818"
     CDS             505..1455
                     /gene="cbl"
                     /locus_tag="QIE41_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDS3533572.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          379 a          322 c          388 g          398 t
ORIGIN      
        1 agattgcccg gggcaaagcg ttgatgttaa tgctattgca caaatgaacc tttttctccc
       61 cagtgattac agcgctgtta gacttcgtgt tgatgaggcg ttttccctac ggcggctcac
      121 ggcaaaagtc attggtgaaa ttgagtctat tgctacgctt accgcagcga ttgccagcgg
      181 catgggcgtt gcagtattac ccgaatcggc cgcgcgttcg ttatgtggcg cagtaaatgg
      241 gtggatgtca cgcattacca cgccttccat gagtctctct ttgtcattaa atttacccgc
      301 cagagcgaac ttatcgccac aggcacaggc agtgaaagag ttgttaatgt cagtgattag
      361 ttctccagtg atggaaaaaa ggcagtggca attggtgagc taagcgttat atcgggatgg
      421 aataagatgc gggtttttat tatttgttat gccgggcgtt agactttaac aataacggga
      481 tatctgaact gcccggagtt taccgtgaat ttccaacaac taaagataat ccgcgaagct
      541 gcacgtcagg attacaacct gacagaggtt gcgaatatgc tttttacctc acagtcaggc
      601 gtcagccgtc atattcggga actggaggat gaacttggca tcgaaatatt tgttcgacga
      661 ggtaagcgac tgctgggcat gactgaaccg ggcaaagcat tactggtcat tgcagaacgt
      721 attctcaatg aagccagtaa tgttcgtcgg cttgcagacc tgtttaccaa cgatacgtct
      781 ggcgttctca ctattgcaac gacgcatact caggcacgtt atagcttgcc agaggtcatt
      841 aaagcttttc gcgaactttt cccggaggtt cggctcgagc taatccaggg gacgccacag
      901 gaaattgcga cgttgttgca taatggtgaa gctgatattg gtatcgccag cgagcgtttg
      961 agtaatgacc cgcagctcgt cgccttcccg tggtttcgtt ggcaccatag tttgcttgtt
     1021 ccacacgatc atcccttgac gcaaattaca ccattgacgc tggaatcaat agcgaagtgg
     1081 ccgttaatca cttaccgaca ggggattacg gggcgctcac gtattgatga cgcatttgcc
     1141 cgcaaaggtt tgctggcaga tattatatta agtgcgcagg attctgatgt cattaaaacc
     1201 tatgttgctc ttgggcttgg gatcggatta gttgccgaac aatccagcgg tgaacaagag
     1261 gaaaagaatt taatccgcct ggatacgcgg catcttttcg atgctaatac tgtctggttg
     1321 ggactgaagc gaggacaact tcagcgtaac tatgtctggc gctttctgga actttgtaat
     1381 gcaggacttt cagttgagga tatcaagcgg caggtgatgg aaaacagtga agaggaaatt
     1441 gattatcaga tatagcaaaa agcccgctat aaaagcgggc ttttcag
//
LOCUS       DAOTRK010000327         1457 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.331, whole genome shotgun sequence.
ACCESSION   DAOTRK010000327 DAOTRK010000000
VERSION     DAOTRK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.331"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(15..308)
                     /locus_tag="QIE41_004819"
     CDS             complement(15..308)
                     /locus_tag="QIE41_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDS3533573.1"
                     /translation="MSLVRYNSKIIITVALISLLSACTRENSLKDTQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(310..810)
                     /locus_tag="QIE41_004820"
     CDS             complement(310..810)
                     /locus_tag="QIE41_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDS3533574.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNTLLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCIRES"
     gene            1069..>1457
                     /locus_tag="QIE41_004821"
     CDS             1069..>1457
                     /locus_tag="QIE41_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDS3533575.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRVLVIVGND
                     VSLGRSDSFSELPENTIVIPYGELTGSFEVIITTDPDLVVTIRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKVKNEVWQYSIPGIV"
BASE COUNT          458 a          225 c          275 g          499 t
ORIGIN      
        1 gtgtagattc ttatttaatg tgttaaacga tcttgcgaaa tgacaagcaa ttgatacata
       61 ctcatctctt tataagctgg aacagtagta acctgcgcaa attcgcgata tagcttctgc
      121 tctagttctg ctgatgatat atattccggg tgttcgctaa agaactgtct tgcatctact
      181 atcgcttgag attcgccact tcttttattc tgagtatcct ttaatgaatt ttctctggtg
      241 catgcagaga gcaacgatat cagcgctaca gttattatga ttttactatt atatcttact
      301 aatgacatat caactttctc ttatgcaaga ttggagttta aaataatgtt tatcccattc
      361 acagagtgct ataaacaact caacagtacc ttttatgtta agtttattta gggctcttat
      421 tttgtgatat gatactgttt tataatcaat tcctttgata atggctattt gttttaacga
      481 ataagaaaca cataaccgca ggatatctat ctcatcatgg gtaagtggtt cttgagtgga
      541 atacacattt tgtgtatgaa gattcgaaac caggaaatgc gttaaatcag ataatgtaat
      601 attcttattg atcctgatga aattgttgct caaaagggtg tttacaggtt ctgtatttgg
      661 tttgagattt cgatgttggt gctcatcaat aacaacgaac gaatgtccaa gatcaaccaa
      721 cgcaaccaaa tcgctgtaat attccttttg ggatgctaat aatgcattag tgcctacagt
      781 gaagtagaca tcactggaaa agacttgcat atgccaacct gcactctaaa atcatttagg
      841 gtaaatatta cgtgtaaaat ttaaaataat ggtaattgtt atatttgcat atcaatgagg
      901 gaatagggtg tataccttaa attgcaaata aagttatgta tgtaggcatt taaaatggtt
      961 ctcgaaaatt ataatctttt tattgtgtgt catcataatt tggtcatgaa atttccgtct
     1021 tgttacacta ctctaaacta gagcttatgg aattagcggg tggtattcat ggaaaataat
     1081 gataaattct tatcacaaga cttattggaa tcttatgcca ttcgtttatt gagcggacca
     1141 ttgaatggat gcgagtatga aatacttaat gggcgtgtac tcgttatcgt tggtaatgat
     1201 gtttcgttag gaagaagtga ttctttttct gagttaccag agaatacgat agtcattcct
     1261 tatggagagc ttacaggcag ttttgaggta ataatcacta ccgaccctga tctggttgta
     1321 acaatcagag aattaacagc acaagaacct gaagatagaa cgttaacatt gaatcagcaa
     1381 atagaagttt taggcctcaa atttgctgta aaagtaaaaa atgaagtttg gcagtattca
     1441 ataccaggta ttgttga
//
LOCUS       DAOTRK010000328         1445 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.332, whole genome shotgun sequence.
ACCESSION   DAOTRK010000328 DAOTRK010000000
VERSION     DAOTRK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.332"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1387)
                     /locus_tag="QIE41_004822"
     CDS             complement(<1..1387)
                     /locus_tag="QIE41_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDS3533576.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTTDEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYKQLNQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMMGSHPNPCDRLHSWPNHYLEQYPRKVACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGMSVSDSANPVHHDGHIQGGYSVPLIITASDIMSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPP"
BASE COUNT          415 a          340 c          348 g          342 t
ORIGIN      
        1 atggcggtat attttcagta cgaatgccgg tcagccactg aaaaatacct gcgaaatgac
       61 gggcactgat ttttctgctg acggactgat gagacatgat gtcactggcg gtaataatca
      121 ggggaacgct gtagcctcct tgtatatggc catcatgatg aacaggatta gcactgtcgc
      181 tgaccgacat cccatggtca gaaaagtaaa gcatggcgaa atgacgggaa tgccggcgaa
      241 ggataccatc aagctgaccg agaaagttat ccagtttact gatgctggcg aggtaacagg
      301 caacctttcg gggatactgc tccaggtaat gattcggcca ggagtgaagc cggtcacacg
      361 ggttcggatg agaccccatc atgtgcagga atatcacctt cggagaggat ttatccgcca
      421 gtgcacgttc tgtttcctgt aacaacaaca tgtcatccgt tttacgggaa gcaaagctgc
      481 ctttcttgag gaaaacggta tgctccgcat cagaagcaat aacagagatg cgtgtgtcat
      541 gctctcccag ttttccctga ttggatatcc accatgtgct gtatcctgct tttgctgcca
      601 gcgccaccac gttgttgccg gaatcagggt tctgctcata gtcataaatc agtgtccggc
      661 tcagggaagg cacagtactg gctgctgccg atgtatagcc gtcaataaat aaaccgggag
      721 cagtattcag ccacggtgtg gttggcacgg gatagccata taccgacata taatccctgc
      781 gcacactctc accagtgacg ataacaatcg tgtcatacaa cggtacaccc ggcaggattt
      841 tccagttgtc agccccgtgc tgattcagtt gtttataacg ctgcatttca cgcaatgtgt
      901 cagttgtccc cacaacagtt cctttaacca tccgcaacgg ccagctgttt actgagcata
      961 atacgaacag cagcagtgcc agccagttac ggtgaccgcg gtggtgtgtt cgccagaaaa
     1021 tcaccatgaa taccagaatc gcggcactga ccagaaaatg ataaacagga atcatcccgg
     1081 taaactccgc tgcctcatca gttgtggtct gcaacaacgc gacaataaaa ctgttgttga
     1141 ttttaccgta cgtcataccg gcaggcgcat acagtgcaca acagaacaga aataacagcg
     1201 ctgtaatgga tgtgagggta tttctgtgtg caaggagcag aagaaggaac agcagcaaca
     1261 cattcccggt ggcattcttc tcagtgtatc cgcatgcaat tgtggttatg acagaaacaa
     1321 caaaaaagaa taaaaacaat aaaatccggg gtgtattgcc cggacaaaac agttttctga
     1381 tattcatcgg aatatatcgg taatattatt ataaagggaa taagataata aaaaccataa
     1441 atctc
//
LOCUS       DAOTRK010000329         1421 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.333, whole genome shotgun sequence.
ACCESSION   DAOTRK010000329 DAOTRK010000000
VERSION     DAOTRK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.333"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1421
                     /gene="ltrA"
                     /locus_tag="QIE41_004823"
     CDS             <1..>1421
                     /gene="ltrA"
                     /locus_tag="QIE41_004823"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDS3533577.1"
                     /translation="QRLLRLITQPEWLAEAARITLSSKGAHTPGVDGVNKTMLQARLA
                     VELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALRDRIVQRAMLMAMEPIWE
                     SDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEGDLSSYFDTVHHRLLMKA
                     VRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLH
                     ERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAE
                     AIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIRKRSRYGEMRVVSTIPQE
                     KARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKVFSYIDRVVFW
                     KLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNHGKLSGEILYRLVGQGKK
                     LFRWRLPEGNPYLRTETRNTYTS"
BASE COUNT          363 a          357 c          403 g          298 t
ORIGIN      
        1 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
       61 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      121 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      181 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      241 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
      301 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
      361 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
      421 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
      481 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
      541 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
      601 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
      661 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
      721 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
      781 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
      841 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
      901 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
      961 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1021 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1081 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1141 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1201 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1261 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     1321 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     1381 tccctatctg aggacggaga ccagaaacac gtatacatcg c
//
LOCUS       DAOTRK010000330         1374 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.334, whole genome shotgun sequence.
ACCESSION   DAOTRK010000330 DAOTRK010000000
VERSION     DAOTRK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.334"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            204..>1374
                     /gene="argH"
                     /locus_tag="QIE41_004824"
     CDS             204..>1374
                     /gene="argH"
                     /locus_tag="QIE41_004824"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDS3533578.1"
                     /translation="MSTKLWGGRFDMPTNKLVEQYNATITLEQRLCPFDIQGSIVHAT
                     MLGRQGIITQDEANTIIRGLRQVSKEIEDGQFIFDTVDEDIHMAIERRMTEIIGPVGG
                     KLHTGRSRNDQTTVDSKMHMRAIIREIQEDITNLQKIIINKAENNINVIMPGYTHLQT
                     GQPILLSHWIMAYYWMLRRDWNRFEDLYQRMGECPLGAAALAGTTFPIDRNFTARELG
                     FDKPTENSIDSVSDRDHMVEFTAAAAMCFMHLTRLSEELILFSSQDFKFIELSDDFCT
                     GSSIMPQKKNPDVAEKMRGKGGRMYGNLMAMLTIMKGIPLAYNTDMSEDKEQVYDSMD
                     TLQASLRIMAPMIEKMVILAENTRAAAARGFSNATDMADYLVRKGIPFREAHHIVG"
BASE COUNT          438 a          258 c          297 g          381 t
ORIGIN      
        1 taacatgtta aacgtgaggt atatctccat gcttatcatt aaaacattaa caaacattat
       61 gatttaatga ttaaaactaa tggcaatata agtcaattta atcgccgcat ttgttgacaa
      121 ttgccaacat cattttataa cctgatatta aatccagccg gtacattttt tctaattact
      181 tatgaataag caggaggatg aggatgtcta cgaaattatg gggcggacgt tttgatatgc
      241 caacaaataa actcgttgaa caatataatg caactatcac gctggagcag cgcctatgcc
      301 cctttgatat tcaaggaagc atagttcatg ccacgatgtt aggacgtcag ggaataatca
      361 cacaggatga agccaataca attattagag gtttgagaca agtaagtaaa gaaattgaag
      421 acggccaatt cattttcgat actgtagatg aagatattca tatggccatt gaaagacgga
      481 tgactgaaat tattggccct gttggtggta aactccacac ggggcgaagt cgtaatgatc
      541 aaaccactgt tgattctaaa atgcatatgc gagcaattat ccgtgaaatt caagaggata
      601 ttaccaacct gcaaaaaata ataattaaca aagcagaaaa caatattaat gtcatcatgc
      661 caggctatac tcatttgcaa acaggtcagc ccattctttt atctcactgg attatggcat
      721 attactggat gttgcgtcgt gactggaata ggtttgaaga tctgtatcaa cggatgggag
      781 aatgcccttt gggggcagcg gctctcgccg gtacgacatt ccctattgat cgtaatttta
      841 cggctcgtga acttggtttt gataagccaa ctgagaatag tattgattct gtcagtgacc
      901 gcgaccatat ggtcgaattc accgcggcag cagcgatgtg ttttatgcat ctaactcgcc
      961 tttcagagga actgatttta ttctctagcc aagactttaa atttattgaa ctttctgatg
     1021 acttctgtac aggatccagc atcatgccgc agaaaaagaa ccctgatgtg gcggaaaaaa
     1081 tgcgtggtaa aggtgggaga atgtatggaa atctgatggc catgctgact attatgaaag
     1141 gcataccgct agcgtataat acagacatga gcgaggataa agagcaggtc tatgactcaa
     1201 tggatactct acaggccagc ttaagaataa tggcacctat gatcgaaaaa atggttatcc
     1261 ttgccgaaaa tacgcgtgca gcagccgctc gaggattctc gaatgcaaca gatatggccg
     1321 attatctggt ccgtaaaggt attcctttca gagaagctca ccatattgtt ggta
//
LOCUS       DAOTRK010000331         1327 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.335, whole genome shotgun sequence.
ACCESSION   DAOTRK010000331 DAOTRK010000000
VERSION     DAOTRK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.335"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            4..660
                     /locus_tag="QIE41_004825"
     CDS             4..660
                     /locus_tag="QIE41_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDS3533579.1"
                     /translation="MDKRKELFESIKDYLLDEANRKADGRIETETELATRFNTSRYKV
                     RQALSTLVQMGMLERAPRRGSLLKNVGQSSINDQLLMQFDLSGFDISEFTEARILVEC
                     AIIPLATRRIFPAMLSKLENELRLIEENADNPQIADQHDRDFHLLLLQASGNRVLQVF
                     SDVLIAYFEKTRESLPDNDCQYFLDTASKQREILSYIKKGDAQMASELMRVHLLEKKI
                     "
BASE COUNT          421 a          234 c          290 g          382 t
ORIGIN      
        1 attatggata aaagaaaaga actatttgaa tctattaaag attatttgct tgatgaagct
       61 aatcgtaaag ccgatggccg tatagaaaca gaaacggaac ttgcaactcg ttttaacacc
      121 tcccgatata aagtccgtca ggcacttagt actctcgtcc aaatgggaat gcttgaacga
      181 gcccctcgca gaggaagtct gttaaaaaac gttggccaga gtagtattaa cgaccaattg
      241 ttgatgcagt ttgatctatc tgggtttgat atctcggaat ttacagaggc cagaatttta
      301 gttgagtgcg cgatcattcc actggcaaca cgccgaattt tcccagcgat gctctccaaa
      361 cttgaaaatg aattgagatt gattgaggaa aatgcagaca atccacaaat agcagatcaa
      421 catgatcggg attttcacct gctcttgctt caggccagtg gtaatcgtgt tctgcaggtt
      481 ttctctgatg tgttaatcgc ttattttgag aaaacaagag agtcgttacc ggataatgat
      541 tgtcaatact tccttgatac tgcttcaaaa cagagagaga tattgtcgta tatcaaaaaa
      601 ggcgatgccc aaatggcttc agagttaatg cgagttcatt tactggaaaa gaaaatatag
      661 cattaaatct ttcaaaaact aaaactcaac cggaaaactt atctggtgtg agtgctatgt
      721 gatatcactg tcaagttatt agctgtgttt atcctaataa ggtatctttg atatataagc
      781 gagctttatt ggtaaagaga gcgctatcat agcagactat gttaagttag agcagggatg
      841 cgtaagtctg agtgccacgt cttctgtacg tatcgacgcc ttatacgatc tggaaaatat
      901 tatcaggcat aaacataaag gagaacttgc ctgagtagtt gccttttgag aaatttatct
      961 tcactggatg ggagttacct gccaacatgt gttcccgtcc aaggacagaa ccaacagttg
     1021 ggtgttgatt acctggtgac gcggaaccaa cagaagttgt aattatgcag gtctgaggag
     1081 atcagtatca gagatatccg aatgaactgt gataatggtt tgacaaaaac ggataacaat
     1141 acaacagaac acgttcttag taatctcagc catggaaata aaaattagat gtatctccaa
     1201 tcagtgagtt gcgtggaaat tacaaggttc tgctgtacga tctgagtggg ttaacttgat
     1261 attttgtact cgtcacagaa aatatacaga gatatacact ctggtgtaat cactgttgta
     1321 ggttgat
//
LOCUS       DAOTRK010000332         1326 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.336, whole genome shotgun sequence.
ACCESSION   DAOTRK010000332 DAOTRK010000000
VERSION     DAOTRK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.336"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..247
                     /locus_tag="QIE41_004826"
     CDS             <1..247
                     /locus_tag="QIE41_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDS3533580.1"
                     /translation="HGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEH
                     LEERRLMLQWWADFLDANREECISPFEYAKVNNPLKR"
     gene            503..>1326
                     /locus_tag="QIE41_004827"
     CDS             503..>1326
                     /locus_tag="QIE41_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS3533581.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERA"
BASE COUNT          358 a          308 c          312 g          348 t
ORIGIN      
        1 ccatggattc cgggcgatgg cgtgcagtgc attgattgaa tccggcttat ggtcaagaga
       61 tgcggttgaa cgtcagatga gtcatcagga gcgcaacgga gtacgtgccg cgtatattca
      121 taaagcagaa catctggaag aacgtcggct tatgctgcaa tggtgggcag attttctgga
      181 tgcaaacagg gaggagtgta tcagtccgtt tgaatatgca aaagttaaca atccactgaa
      241 aaggtgactg aacgccattt acacacatgc tcttcctctt aaggggaaga gcacccgcct
      301 caatcaattt cttttttatg ccggcggaac catcagccag gctgaggttc tcattcaata
      361 actcaacgtc ttaccacaga acagaattca ctgtattatc atgggcaacg ttaatcccgg
      421 acaataaaag gacaccgatg ccaggcatca caatttttat agcctgcagg ttatggtcca
      481 tcctttttta caggttcaga atatgactgg caaattacgc tttgaagtga acgataacca
      541 gggctgtttc atatttccgg aaacctggtt tggctcgttg ctggatgagt ttgaagaact
      601 gattgatgca tacgatgcgg atgaaatcag cgaaacaagt tacatcaaca aactcaggcg
      661 ccttgcccga caggagaatg actttattga tgtacacgct cacttggcat acgtatttct
      721 ggagcaaaat gccccgcgta aggcgctgaa tgctgccctt aaagggctgg caattggcaa
      781 tcgtcttatc cctgaaggtt tcagtggcag aatcatctgg atacatcctg acaaccgacc
      841 ttttcttcgt gccctctatg cggcaatcct ggcaaatgca catttacagc gtcatcagga
      901 tgccatcatg ctcattgaaa aaatactgga ttacaatccc gaagataatc atggtgcacg
      961 ctggctactg ggaccggaac tgttacgcac tggcgctcat gaacaggcac gccatatact
     1021 gcaggaacat gcagatgaat tttcacctta ctggtatgaa ctcggccttc tgcattttct
     1081 taatggggaa ctggtaaaag ctgcaactgc atttcgtcgc ggctttgcgg caaatactta
     1141 catcgcagaa atactgtgcg gaaatctgca tccatttcca cttgctgtat ggcataactt
     1201 ttcaggcggt ccggatactg ccgaagatta ttatgccaca taccacccgt tatgggggca
     1261 atatccggaa gcattgctgt ttgtaaactg gctctataat cattcctctg tgctgcatga
     1321 acgggc
//
LOCUS       DAOTRK010000333         1322 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.337, whole genome shotgun sequence.
ACCESSION   DAOTRK010000333 DAOTRK010000000
VERSION     DAOTRK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.337"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..332)
                     /locus_tag="QIE41_004828"
     CDS             complement(<1..332)
                     /locus_tag="QIE41_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533582.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKYVNPVKITMRSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFPITDDLMSMATNELIKLA
                     KIENNLDL"
     gene            762..1187
                     /locus_tag="QIE41_004829"
     CDS             762..1187
                     /locus_tag="QIE41_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533583.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWVLNSRYVNVNSVSKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            1223..1303
                     /locus_tag="QIE41_004830"
     CDS             1223..1303
                     /locus_tag="QIE41_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533584.1"
                     /translation="MKDVDQNFDALYWHILQAYLILLFYD"
BASE COUNT          413 a          203 c          211 g          495 t
ORIGIN      
        1 ttcaggtcta aattattctc tattttggcc agttttatga gttcatttgt agccatactc
       61 attaaatcat ctgttatcgg aaatttatat atgctcatag ccgataatat tgcttgctga
      121 attatttctt cagaaatatc cgctatcttt attgacaaat catgtgttag atattttcct
      181 ccaagagatt cttgttcaat ataatttaaa ccagacctca tcgtaatttt aactggattc
      241 acgtatttct cttcattttt cttatttttg ttctcctgat caggaactaa tggttgcagt
      301 ggtaggctat tattaatatt tcgtggcttc attttacatt tctctcattt gattagagca
      361 aaatccgaat tcgcagcttc tgataatgtc aaatatcacc ttttctcatg ctttattcta
      421 tcaaaaatcg ctcattttta ctttttttac cataatgtta cttagtggca tcggcaattt
      481 acataactta catgttgttt tttatatcct gaaaaaacag atagatagtg tgcacactcg
      541 aaatgatatt ggtaacataa atagctttat tcgtgacaaa catctcaaaa gatttccttt
      601 attattttcg aaattcattt gatcaaggtt atcaccttat ggagacgtta cgtagaatga
      661 ctatatttgt tatcggcaca ttaggggtat taaatttagt attgatggaa ggtgattatt
      721 gaacagtaac atcgagtcac ggtcaaaatg tgaggtatat aatggacatt gagttttcgc
      781 agattcatga aatggtctat atgcatgata ttgtaaattc ggactcgaaa aagaaaccaa
      841 ggattccgtt aaaaaaattc cttaatgctg agaaagttct cacgcaaaca acatcatggg
      901 tactaaattc aagatatgtg aatgttaatt ctgtcagtaa agtcaacgta aagagtaaag
      961 ttaaaaacag ttatatctca cgatctgtca atgatgagtt tagtttaaca gatgatgaaa
     1021 tcaattcttt taaagaaacg ttagtccttt cctctattga ttctctaagt aaactggttc
     1081 ttaataatcc tctttctgta ttgtttacat cgactgtacg aaggaataat aatagagcta
     1141 agatgaatgt tgagttcgac tcatggattt gcaccagatg ttgttgatac atgatatttt
     1201 gcattgcaag gtgatatcta aaatgaaaga tgttgatcaa aactttgatg ctttatactg
     1261 gcatatacta caagcatatt taattttatt gttttatgat taacgaggtt ccatcttttt
     1321 at
//
LOCUS       DAOTRK010000334         1283 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.338, whole genome shotgun sequence.
ACCESSION   DAOTRK010000334 DAOTRK010000000
VERSION     DAOTRK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.338"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1283
                     /locus_tag="QIE41_004831"
     CDS             <1..>1283
                     /locus_tag="QIE41_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDS3533585.1"
                     /translation="LDRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLKAQTD
                     AAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAHNGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDAWGKPVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITQRTVNGEPAEQWQYDERGWL
                     TGISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRC
                     IPDSLPAVEWLTYGSGWLSGMKLGDTPLVEYTRDRL"
BASE COUNT          311 a          374 c          401 g          197 t
ORIGIN      
        1 ctggaccgcc gtgaagtgct gcacacgcag ggcgaaggcg ggctgaagcg ggtggtgaaa
       61 aaggaacacg cggacggcag cgtcacgcag agtcagtttg acgcggtggg caggcttaag
      121 gcacagacgg atgccgcagg ccggacgact gagtacagcc cggatgtggt gacgggactc
      181 atcacgcgca tcaccacacc ggatggcagg gcatcggcat tttactataa ccaccacagc
      241 cagttaacgt cagccaccgg gcctgacggg ctggaattgc gccgggaata tgatgaatcc
      301 ggccgtctga ttcaggaaac cgcacacaac ggcgatatca cccgctaccg ttatgataat
      361 ccacacagtg acttaccctg cgcaacggag gatgccaccg gcagccggaa aaccatgaca
      421 tggagccgtt acggtcagtt gctgaccgtc actgactgtt ccggttatgt aacccgttat
      481 gaccatgacc gttttggtca gatgacggcg gtgcaccgcg aggaagggct gagtcagtac
      541 cgcgcatacg acagccgtgg acagttaatt gcggtgaaag acacgcaggg ccatgaaacg
      601 cggtatgaat acaacgccgc cggtgacctg accgccgtca ttgccccgga cggcagcaga
      661 aacgggacac agtacgatgc gtggggaaag cccgttcgta ccacgcaggg cggtctgacg
      721 cgcagtatgg aatacgatgc tgccgggcgg gtcatccgcc tgaccagtga aaacggcagc
      781 cacaccacct tccgttacga tgtacttgac cggctgatac aggaaaccgg ctttgacggc
      841 cgcacacagc gttatcacca cgacctgacc ggcaaactta tccgcagcga ggatgagggg
      901 ctggtcaccc actggcacta tgacgaagca gaccgcatca cacagcgaac ggtgaatggt
      961 gaaccggcag agcagtggca gtatgacgaa cgcggctggc tgacaggcat cagccatctc
     1021 agcgaaggcc accgggtgac ggtgcattac gggtatgatg agaaaggccg cctgaccggt
     1081 gagcgccaga cggtgcatca cccgcagacg gaagcactgc tctggcagca tgaaaccaga
     1141 cacgcttaca acgcgcaggg actggcgaac cgctgtatac cggacagcct gcccgccgtg
     1201 gaatggctga cctacggcag cggctggctg tcaggcatga aactcggcga cacaccgctg
     1261 gtggagtaca cccgcgaccg cct
//
LOCUS       DAOTRK010000335         1276 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.339, whole genome shotgun sequence.
ACCESSION   DAOTRK010000335 DAOTRK010000000
VERSION     DAOTRK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.339"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            430..505
                     /locus_tag="QIE41_004832"
     tRNA            430..505
                     /locus_tag="QIE41_004832"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:463..465,aa:Ile,seq:cat)
BASE COUNT          418 a          209 c          256 g          393 t
ORIGIN      
        1 aatctgtttt tttaattatc gatgtggtct ggagaggcaa ctgtcaaaaa tcgctcatac
       61 gaaactaatg attagcagaa taagatctat gaaagaatat attgctatat gaagtttatt
      121 tttatggggt gaaaagtaac agatatcata atgatgcgaa aactcttcaa ctaatgtaat
      181 aaagttttat tattagatac aaaatcatta caaactttca ttgaatcgat ggtaactata
      241 aatagtgcta agtattagca tttgcttgtt gactgagagt atcgactttg tgaatggaag
      301 cggtgtcggg ttttatgtcg caggtaatga cgacccagga tgaatgagaa cgcataaaaa
      361 tctgttattt acatcataag atcatcatgt ttattgcgtg gtttataagt attgttattg
      421 catactcaag gccctttagc tcagtggtta gagcagacga ctcataatcg tccggtcgct
      481 ggttcaagtc cagcaagggc caccaaatca tcattcaggt atcaggaaga gttaagggta
      541 caaggggtac tcgatggcgg tctatatttc acgactaacg ccatttcaag gaagaagtgt
      601 ggtgggtggt ttcgaatgag ggctggtgtt ttggtacgtg gcacgccact gcaatgtatt
      661 tatccatcag tactcattca aaagtacctg gaaaaaatgc ccgctatgcc aagcgggcaa
      721 attgcttaca tgtggaatta agtaattaac gataaagcca gagtattatc atttatcatg
      781 cctcttcatt taatcaggtt aattactcaa atcactgaac tattctctgt tcgctcactg
      841 atgataagtg aagtgtcaca accacgattt gtatgtggac caatgctgta gagtaaggat
      901 gaaatgtctc tgccacgcct taaacatcta ctactgacta gaaccgccgt cgaatgcaga
      961 agagtcttgt gagcattaca ggagtctggc tttggtcatt aatcaacgct atttagatat
     1021 aaatttctaa cactcaaaaa acgattctca cgaataaatt ataacgggtg gtttttatca
     1081 tacagtgaaa taaccttttg cttattaaat ttaacattct tgtgctaaat taattaaaag
     1141 taccatgtat agcatatgaa gctgttaatg ccagcaattg tctcaaatga gcgatttttt
     1201 atagtgacaa acaataataa cattaaaaat agccatgtag acattaactg aggaaaagac
     1261 aatgttagtt agtaaa
//
LOCUS       DAOTRK010000336         1276 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.340, whole genome shotgun sequence.
ACCESSION   DAOTRK010000336 DAOTRK010000000
VERSION     DAOTRK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.340"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>1276)
                     /locus_tag="QIE41_004833"
     CDS             complement(<1..>1276)
                     /locus_tag="QIE41_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HDS3533586.1"
                     /translation="PNDNEKLSVYLKNKIPFENITIDLSALEKEALASLKYCNFKNVT
                     FTGNISYENLNGSVFENCFFEGCKFENVSLISSRCRVKLNNEIPIYGVFKECFLYRCE
                     IKNFNIDTSKIYSVNQDPNVGKQVGAYLFMQSFVYECTLLNGVCKNASIIASSLLCCK
                     IAKLNGAEMDFIGTSFYHKYRCELKYDNHFYMCDFSSSDMTCKRNGIRKDLLDFDPRE
                     YFKKDGGKLNLDKHISNLIYVNLFPNMSGGRYINDKYVADDIDKYLKLSNCNLLKTCL
                     GESINGIKLDDCAIDPSTTWLSGNLAANSQIRIPVYMDMKGAIAENGISDFMENLMEV
                     NSWVEEFNNYGKDAISHYEKELLYLQSLLVKLGSFNLDDEGKKYQLNGEAIQYIILNV
                     LYNIEKDIANEIDIDKVNFMHNLYTGFFWQRHY"
BASE COUNT          429 a          232 c          159 g          456 t
ORIGIN      
        1 cgtaatgtct ttgccaaaag aatccagtat agaggttatg catgaaattt actttatcaa
       61 tatctatttc attggctata tctttttcta tattgtacaa aacatttaat ataatatact
      121 ggatagcttc accattaagt tgatattttt tgccttcatc atcaagattg aagctaccca
      181 gcttgaccaa caaagattgt aaatataaca gttccttttc gtaatgactt atcgcatcct
      241 tgccataatt attgaattct tctacccatg aattaacttc cattaaattt tccatgaaat
      301 ctgaaatgcc attttcagct atagctcctt tcatgtccat ataaacagga attctaatct
      361 ggctattcgc agcaagatta ccacttaacc aagtggttga aggatcaata gcacaatcat
      421 ccaatttaat tccattgatt gactcgccca aacaagtttt aagtaaattg caattactaa
      481 gttttaaata tttatctata tcatcagcaa cgtatttatc atttatataa cgaccgccac
      541 tcatattagg gaaaagatta acgtagataa ggtttgaaat gtgtttatcc agattaagtt
      601 tacctccatc ctttttgaaa tattctctgg gatcaaaatc cagtaaatct ttgcgaattc
      661 catttcgttt gcatgtcata tcagatgagc taaaatcaca catataaaaa tgattatcat
      721 attttaattc acatctatat ttgtgataaa acgaagtccc tatgaaatcc atttcagcac
      781 cattaagctt tgctatttta caacataaca aactgcttgc aataatactt gcatttttac
      841 acactccatt cagaagagta cattcatata caaatgattg cataaacaga tatgcaccaa
      901 cttgcttacc cacattggga tcttgattta cagagtatat ttttgatgta tctatgttaa
      961 aattttttat ttcacatcga tacagaaaac actctttaaa cacgccgtaa ataggtattt
     1021 cattatttag ttttactcga cacctggagc ttataagtga cacattctca aatttacagc
     1081 cctcaaaaaa acaattttca aatactgaac cattaagatt ttcgtaagat atattaccag
     1141 tgaatgtcac atttttaaag ttacaatatt ttaaagatgc cagggcttct ttttccagtg
     1201 cagataaatc aattgtgata ttttcaaagg ggattttatt ttttaaatat acgctaagct
     1261 tttcattatc atttgg
//
LOCUS       DAOTRK010000337         1274 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.341, whole genome shotgun sequence.
ACCESSION   DAOTRK010000337 DAOTRK010000000
VERSION     DAOTRK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.341"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1274
                     /locus_tag="QIE41_004834"
     rRNA            <1..>1274
                     /locus_tag="QIE41_004834"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          351 a          272 c          401 g          250 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 atgtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1081 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatat
     1141 gtaggtgaag cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac
     1201 tgtttattaa aaacacagca ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct
     1261 gcccggtgcc ggaa
//
LOCUS       DAOTRK010000338         1273 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.342, whole genome shotgun sequence.
ACCESSION   DAOTRK010000338 DAOTRK010000000
VERSION     DAOTRK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.342"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..257)
                     /locus_tag="QIE41_004835"
     CDS             complement(<1..257)
                     /locus_tag="QIE41_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDS3533587.1"
                     /translation="MQDIRQETLNECTRAEQSASLVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYG"
     gene            complement(257..586)
                     /locus_tag="QIE41_004836"
     CDS             complement(257..586)
                     /locus_tag="QIE41_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3533588.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(583..>1273)
                     /locus_tag="QIE41_004837"
     CDS             complement(583..>1273)
                     /locus_tag="QIE41_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDS3533589.1"
                     /translation="LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAA
                     MLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQA
                     AAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYV
                     GTPGSLADSRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQM
                     RDGGLFSGGGR"
BASE COUNT          263 a          428 c          300 g          282 t
ORIGIN      
        1 ccgtacaggt tagaaaccgt cagcgtgggg cgcgtactgg tgcctttgcc attcagttca
       61 aaaccgctcc cctgaatggg atacggctga tactgtcgcc cctgccaggt gaccggctca
      121 cctttttcgt tctgctcatt acagaaaaaa taacgttctc caccgacctc tgtcaggtcg
      181 atttcccaga gcaccaagct ggccgactgc tccgcacggg tgcattcatt cagtgtttcc
      241 tgccggatat cctgcatcag ttcaccacct gttcaaactc tgcgctgaac tcaacacgca
      301 gcatactgac ccgcgacgac cattttgcgc aggtcacctt tatctgccgc cactcataag
      361 gcggcgtcca cagaaaggat ttccagcccc cgtgctcttc cagaaacgac tccagtacca
      421 tggcctcctc acgggggaca gaaagcgtca cgctgtacgt tttcaggttg gcattcagcc
      481 cggcaggcgc tcgctgggaa tagccatcac caaagcgcac ctttcttaca gaaggggccg
      541 aagccacatc cataccgggt ttcactttcc agcggaaggt tttcatcgtc cacctccgga
      601 gaacaggcca ccatcacgca tctgtgtctg aatttcatca cgggcaccct tgcgggccat
      661 gtcatacacc gccttcagag cagccggacc tatctgcccg ttcgtgccgt cgttgttaat
      721 caccacatgg ttattctgct caaacgtccc ggacgcctgc gaccggctgt cagccagact
      781 gcccggtgta ccgacataac caccggtggc atagccgcgc atcagccggt agagattccc
      841 cacgccaatc cggctggttg cctccttcgt gaagacaaat tcaccacggt gaacaatccc
      901 cgctggctca tatttgccgc cggttcctgt gaatcctccg gttgcaaaat ggaatttcgc
      961 cgcagcggcc tgaatggctg taccgcctga cgcggatgcg ccgccaccaa cagccccgcc
     1021 aatggcgctg ccgatactcc cgacaatccc caccattgcc tgcttaagca aaatttctgt
     1081 catcatggac agcacggatc gggtgaagct gcgccagttc tgttcactgc cggtcagcat
     1141 cgccgccata ttctgtgcaa taccatcaaa ggtctgcgtg gctgcacttt taacctgcga
     1201 catactgtcc gtggcgctct cttcccactc actccagccg gacttcaggc ctgccatcca
     1261 gttcccgcga agc
//
LOCUS       DAOTRK010000339         1247 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.343, whole genome shotgun sequence.
ACCESSION   DAOTRK010000339 DAOTRK010000000
VERSION     DAOTRK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.343"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1247
                     /locus_tag="QIE41_004838"
     CDS             <1..>1247
                     /locus_tag="QIE41_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS3533590.1"
                     /translation="FKYISKALELEPSNIQWKEQLEQCARHLDKLNNHMVSKSSTEED
                     LLREKLNTDFNNPKLHDRLAEVLHKKGRWWQEVDTLKNAIKLDPSIPERYFKLGTALE
                     KMGRFNEASIYYKKGLEFNKKHDAIWYYALGYSLESDETNNVENVRSSKIAYATAISL
                     DKKLNSNKFGIGAFHQYKGRWAKAIDAYEKHTSINPLCAELYYRLGLSYDRCYQWDKA
                     AENYRKALSLDENHPYWHYRLGFVLERSQKYLDAAVAYQFAAQSNIKHISIWYYRSGY
                     AYAKANKYRQSCEMYIKAYDSSQEIYASQIKVIDNNDTHLNSYRKKLKYESRINTLES
                     AISNSCINYGLWYELGKTYESSCNWNDAVIAYHEGIMRNNTYTKDYYYRIGYSLFRDG
                     NYQQACNFFRKIEIFGTPYGAPD"
BASE COUNT          466 a          177 c          232 g          372 t
ORIGIN      
        1 atttaaatat ataagtaaag cattagaatt ggaaccctca aatatacagt ggaaagaaca
       61 actcgaacag tgtgcaaggc atcttgataa gttgaataat catatggtga gcaaatcatc
      121 tactgaagaa gatttattaa gggaaaaact taatacagac tttaataatc caaaattaca
      181 cgatagactt gctgaggttc ttcataaaaa agggcgttgg tggcaggaag ttgatacgct
      241 gaaaaacgca ataaaattag atccttcaat acctgaacgt tatttcaaac ttggcaccgc
      301 attagaaaag atggggcgtt ttaatgaggc tagtatatat tataaaaaag gacttgaatt
      361 taataaaaaa catgatgcta tttggtatta tgcactcgga tattcgcttg aatctgatga
      421 aacaaacaat gtagaaaatg tccgtagttc taaaatagca tatgcgacag ctatatcgtt
      481 agacaaaaaa ttaaatagta ataaatttgg aataggagca tttcatcaat ataagggacg
      541 ctgggctaag gctattgatg cgtatgaaaa acatacatcc ataaatccat tatgtgcaga
      601 gctatattat aggctcggat tatcttatga tagatgttat caatgggata aagccgcaga
      661 gaattatcgc aaggcattat cactcgatga aaatcatcct tattggcatt ataggctagg
      721 gtttgtttta gagagaagtc aaaagtatct tgatgctgct gttgcttatc aatttgcagc
      781 acaaagcaat atcaaacata tctcgatttg gtactataga tctgggtacg catatgctaa
      841 ggctaataaa tacaggcaat cctgtgaaat gtatataaaa gcatatgact cttctcagga
      901 aatttatgca tcacaaataa aagttattga taacaatgat acccatttaa actcttacag
      961 aaaaaaactt aaatatgaaa gcaggattaa tactttagag tcagcaattt ctaattcatg
     1021 cataaattat ggattatggt atgaattagg taaaacatat gaatcttcat gtaattggaa
     1081 tgatgctgtc attgcatatc atgaaggtat tatgagaaac aatacatata ccaaagacta
     1141 ttattataga atcggatatt cattattccg cgatgggaat tatcaacagg catgtaattt
     1201 ttttcgtaaa atagagattt ttggaacacc gtatggggct cctgatg
//
LOCUS       DAOTRK010000340         1217 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.344, whole genome shotgun sequence.
ACCESSION   DAOTRK010000340 DAOTRK010000000
VERSION     DAOTRK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.344"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1119
                     /locus_tag="QIE41_004839"
     CDS             <1..1119
                     /locus_tag="QIE41_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDS3533591.1"
                     /translation="LKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFT
                     WQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGI
                     SIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVINNVNVHYPLEVYH
                     FLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVK
                     NDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKIG
                     NINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRITKVNNETVSYFCEG
                     YKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(1085..1207)
                     /locus_tag="QIE41_004840"
     CDS             complement(1085..1207)
                     /locus_tag="QIE41_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533592.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          367 a          236 c          241 g          373 t
ORIGIN      
        1 ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg
       61 atggatgaca gcactctgaa agagttcatc aaacaatata tcgcagcgtc tggcaatcag
      121 gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa
      181 gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg
      241 aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga gtttctggtt
      301 ggtatctcga tcgatggacc ccaggagcta catgaccgtt acagacgcag taattctggt
      361 aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag
      421 tttaatacgt taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat
      481 tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctcga aaccgggacg
      541 ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg
      601 cctcctacgg cttatggcaa gtttatgtcg accattttta tgcaatgggt taaaaacgat
      661 gtgggtgaaa ttttcatccg tcagtttgaa agttttgtca gccgtttttt ggggaatggg
      721 cataccagtt gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga
      781 gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa
      841 tctgaactta aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg gatttcagcg
      901 aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgccctaa gcatcgtatt
      961 actaaagtaa acaatgagac tgtttcctat ttttgcgaag gttataaaat ccttttttca
     1021 accatggtac cttatatgaa cgccatggtg gagttagcca agaacagagt accgctttac
     1081 cacattatgg atgttgcaaa acaaatggag aataattaat aacgctcctt ctttgctcca
     1141 gtcttagcct ccgtcttatg accttaacaa agcacccgac ataaattcct gttgggtgtt
     1201 tcgtcataat catcttc
//
LOCUS       DAOTRK010000341         1211 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.345, whole genome shotgun sequence.
ACCESSION   DAOTRK010000341 DAOTRK010000000
VERSION     DAOTRK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.345"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(112..744)
                     /locus_tag="QIE41_004841"
     CDS             complement(112..744)
                     /locus_tag="QIE41_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDS3533593.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
BASE COUNT          315 a          287 c          305 g          304 t
ORIGIN      
        1 agatccgtaa tgcctgatgc gccatgttta tcaggccaac ggtagaagtg taatctattg
       61 aatttacggg ccggatacgc cacatccggc acaagcatta aggcaagaaa attattcgcc
      121 gtcctgcgtt tcttctacag gctgcatctc gctaaaccag gtatccagtt tctgccgcag
      181 cttgtccacg ccttgtttct tcagtgaaga gaacgtttca acctgcacat caccgttaaa
      241 cgccagcacc gcttcacgca ccatattcaa ttgcgcttta cgtgcgccgc ttgccagttt
      301 gtccgctttg gtcagcagca ccagcacggc gatattgctg tctaccgccc actcaatcat
      361 ctgttgatcc aaatctttca gcggatggcg aatatccatt agcaccacca gaccttgcag
      421 gctctgacgt ttttcgaggt attcgccgag cgcgcgttgc catttgcgct tcatctcttc
      481 cgggacttcc gcgtagccat agccgggcag gtcaaccaga cgcttgccgt cagccacttc
      541 aaacaggttg ataagctggg tgcgccctgg ggtttttgag gtacgagcca ggcttttctg
      601 gttagtcagc gtgttcagcg cgctggattt acctgcgttg gaacggcctg caaaagccac
      661 ttcaattccg gtatcggaag gtaggtggcg aatatcaggc gcactcatca caaaatgcgt
      721 ctgttgataa ttcaaattag tcaaagcggt cgtctccgtc agtcaaagct ttgcggcgat
      781 tatacctgaa ccataataaa aggcggattt ttcggcgtga gcgttgtaag taaaagccat
      841 acgctttgtg agacattgcc gatagtcttt atgcgaaata gcagagaaaa ttctgcgatg
      901 catgtcaaat aagctatata aatcagttaa ttgcttttat ataaaactct gaaaatgtgc
      961 aaatactgat ggcggttgat tgtttgttta aagcaaaggc gtaaagtagc acccatagag
     1021 cgaggacgct aacaggaaca atgactcagg atgagggtca ggagcgccag gaggcgaaga
     1081 cagaggattg tcaggaagac aaacgtccgg agacgtaatt aaacggaaat ggaatcaaca
     1141 cggattgttc cggctaatgg aaaaacaggg tgtgttggcg gcctgcaagg attgtaagac
     1201 ccgttaaggg t
//
LOCUS       DAOTRK010000342         1209 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.346, whole genome shotgun sequence.
ACCESSION   DAOTRK010000342 DAOTRK010000000
VERSION     DAOTRK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.346"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..468
                     /locus_tag="QIE41_004842"
     CDS             <1..468
                     /locus_tag="QIE41_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HDS3533594.1"
                     /translation="TAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHS
                     PVVKDFLAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVK
                     ALAEAGYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKLNRPGNPGD"
     gene            494..1209
                     /locus_tag="QIE41_004843"
                     /pseudo
     CDS             494..1209
                     /locus_tag="QIE41_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    775..891
                     /locus_tag="QIE41_004843"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          311 a          293 c          348 g          257 t
ORIGIN      
        1 accgcaaaac gttacgcggc aacaatcagg cttcgcctga acacgctgga taaaagcgcg
       61 tggacattga ttgccgaagg tagtgaacgg gaacgccaac aacttctgtt tgtggctctg
      121 atgctacatt cgccggtagt taaggatttt ctggctgaag tggtgaacga tctgcgcagg
      181 cagttcaagg aaaagttgcc tggcaatagc tggaacgaat ttgtgaatag ccaggttcgc
      241 ctacatccgg tactcgccag ctactcagat tcatctattg caaaaatggg aaacaatctg
      301 gtgaaggcgc ttgctgaagc gggttatgtg gatacgcccc gcagacgtaa cctgcaggca
      361 gtttaccttt taccggaaac tcaggcagtg ttacagcgcc tgggacaaca ggacttgata
      421 tctattctgg agggaaaact gaaccgcccc gggaatcctg gagactaaac ttcctgagaa
      481 agaggtaaac aggatgacta aaaatactcg tttttccccc gaagtccgtc aacgggcagt
      541 ccgtatggtt ctggaaagtc agagcgaata tgactcacaa tgggcgacaa tttgttccat
      601 tgctccaaag attggctgta cgccggagac tctgcgtgtc tgggttcgcc agcatgagcg
      661 ggataccggg ggcggtgatg gagggctcac caccgctgaa cgtcagcgtc tgaaagagct
      721 ggaacgtgaa aatcgtgaac tgcgccgcag taacgatatc cttcgccagg cttccgctta
      781 ttttgcgaag gcggagttcg accgcctctg gaaaaaatga tgccactgct ggataagctg
      841 cgtgagcagt acggggtcgg accgctatgc agcgaactgc atattgcccc gtcaacgtat
      901 taccactgtc agcaacagcg acatcatccg gataaacgca gtgcccgtgc gcagcgcgat
      961 gactggctga agaaagagat acagcgcgta tacgatgaaa atcacaaggt atacggtgtg
     1021 cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag tggccagatg cactgtggca
     1081 cgtctcatgg cggttatggg acttgccggt gttctccggg gtaaaaaggt ccgtacgacc
     1141 atcagccgga aagccgttgc cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga
     1201 cctgaccag
//
LOCUS       DAOTRK010000343         1163 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.347, whole genome shotgun sequence.
ACCESSION   DAOTRK010000343 DAOTRK010000000
VERSION     DAOTRK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.347"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..571)
                     /locus_tag="QIE41_004844"
     CDS             complement(<1..571)
                     /locus_tag="QIE41_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDS3533595.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEDW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGD"
     gene            complement(675..986)
                     /locus_tag="QIE41_004845"
     CDS             complement(675..986)
                     /locus_tag="QIE41_004845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533596.1"
                     /translation="MEIVIYITISILILAIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCT
                     K"
BASE COUNT          333 a          251 c          230 g          349 t
ORIGIN      
        1 aatcacctcc ctgtttttgt atcttctctg caatatcttt ctggcaaccc atcgcatcag
       61 ttgtgatgat ttttccttta atatccagca tgttaagaag ttctgggata gctgtaatct
      121 cattggattt ctcatccgtc ctgatctgtc cgatgaccag actgtgcatt gttgagaacg
      181 cactaatgac atgaatcgct cccctgcggc gactcttgtc ataagagtgc cggagcgttt
      241 ttccatcaat tgcaatgacg tctttatcat ctgaagaatg gcagtcacgc atccagttaa
      301 taaagcactc gtgaaatttt gcaggactga tacaggatac aactctggca atggtatcgt
      361 gaacaggaat accattttca aaatcaccat attgcttcaa aaaatcgaga tgtgtttccc
      421 caaaatcctc tatatcttcc caatcttccg caccagaaat aacggcacaa atagtcaaca
      481 atagaatatc cgataattta tgttccactt tccaggcttg tctgtaatcg ggaataatag
      541 aaatatgttc catcaatttt ttaagttcca ttttgttctc cttaattatg taaggagtat
      601 ttgatcatgt ataagcaata aaaaacagct tcaggtaatg aggaatatct caatttttaa
      661 acataaaatg tgaattattt agtacaaaaa gtgtacttct gatgatcttt ccctgtatac
      721 atccggtctg cggggctgtg tgccaggcgg tcatcgtcgg tatcgccggt cacctggtgg
      781 cgcagacgca ttcccggaaa ggcgtcataa aagaagcggc agctcagggc atcacggtcg
      841 aacagcacca ggtaatcatc gcggcggcgc aggatgtact gggcgtgata cgggttactg
      901 gtttcgatat tggctaacat ccgctgtttt attaatgcga tagctaaaat caggatggaa
      961 atagtgatgt atatcactat ttccatgatc ttgccttgcg ttcatccagg caggaatatc
     1021 cctgttagca gcctctgtgc gatgtacgcc agcggtctgt aaagaaaccg attaccttat
     1081 aacaaaacct ctaacgtagt ttataataca tcaggaatat taataaaacc ttctgggtca
     1141 gatatctcaa taaatatcat atc
//
LOCUS       DAOTRK010000344         1148 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.348, whole genome shotgun sequence.
ACCESSION   DAOTRK010000344 DAOTRK010000000
VERSION     DAOTRK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.348"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            303..452
                     /locus_tag="QIE41_004846"
     CDS             303..452
                     /locus_tag="QIE41_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDS3533597.1"
                     /translation="MEKVISHTRYKLTPAELEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
BASE COUNT          292 a          286 c          299 g          271 t
ORIGIN      
        1 cgattagatt tggcaaggtg tatgcatcca gcagcttaac acctcagcta actggctggt
       61 taccttcagg ttgtcgcaat ggcatcggac atccggtgtc ggataactaa acgtagcgtt
      121 aaggcgatat tgctgagaga ccggttaagt ggtggcacga gagtaatgag tctggggagg
      181 gtaccagaat gccagtctgg tgcggtatgc cggcagtatc acgttaaccg catcataaag
      241 tgatgagagg ctaatagcgg ttaaaaccag agcagttccg tcgttgttca tcccgggaga
      301 cgctggaaaa agtgatttcc cacacacgtt ataaacttac ccctgcggag ctggaagcct
      361 ttaactctgc ggtcgatcac cgcctggcag aactgacaat gaacaaactt tacgatcgtg
      421 tgccggcttc cgtctggaaa tatgtcacct gaatcttgaa tccgcgctac gatggctccg
      481 gtgttatctg tgcaccggaa gatcgggaac attaagcagt ataacggcaa caaagaattc
      541 gaaatcaaaa gggaatggtg tacagagggg caggcgttca ggcattaccg gcctgtatct
      601 tctgaagggg actcgtattt gtcggggctg agtattccgc tcgattcgat cacggattgg
      661 cggaatacct gactgtgatt gattcaggta ccattttgca gggatccggt gcgttactgt
      721 ctggcacaac aaacgcagcg ggtcgctctg ccccgccgat tcctgataat cgctcccctg
      781 taacctactg caacagtaca aatacactga cgcctgaaac ggtaaccaga atcagcacca
      841 gcagtaacgt cagtaaacag agcagacgat cggtcttccg gtaataaccg aaggggtcga
      901 cagcgccaca atacgggcaa cacactgtat tatgagtaat tgcgttacca cagtccctgc
      961 acttcttttt gcgtatcacg atcgctccct ctccacgcct tatccgtaac ctgtttttta
     1021 cattaaaaaa acaacgccgg gaattcaatc gtcagtccgg cgacgaccgt tcgggttatc
     1081 acagagtgcc tgagacagtg tcctgccgga ggtcacctca ggggctctgc gcatttttta
     1141 cgacgtgg
//
LOCUS       DAOTRK010000345         1144 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.349, whole genome shotgun sequence.
ACCESSION   DAOTRK010000345 DAOTRK010000000
VERSION     DAOTRK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.349"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..805
                     /locus_tag="QIE41_004847"
     CDS             <1..805
                     /locus_tag="QIE41_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HDS3533598.1"
                     /translation="LNMMNDSAEGFCKIISGFPDYNEKTERYDLIIKTWCGESGWLPS
                     EYEYNEYIDSMRELEPNVPQLLDSARISCFTNTALNPTMWAHYANNRTGICIEFDQDK
                     IRELEDIYIFDVKYKDRPPTIDTAVLTLIADQVNYNHDAIYDDYQEKFKHLYEQAFDE
                     GRNLLLEIYNGILATKSQEWSYEQEKRLIYILSFSQEDKLAKKYKTGINISLPEGCIK
                     SVILGEKTSEHDKKLIVSAIKKHSKHIKLKRATITQGEYLLSIRDEVFS"
BASE COUNT          423 a          184 c          192 g          345 t
ORIGIN      
        1 tctcaacatg atgaatgaca gtgctgaagg tttttgtaag ataataagtg gtttcccaga
       61 ttataatgaa aaaactgaga gatatgattt gataataaaa acatggtgtg gagagtctgg
      121 ctggctacct tctgaatatg aatataacga atatattgat tcaatgagag aactggaacc
      181 aaatgtcccg caattgctgg actctgcaag gatatcatgc tttacgaata cggcgttaaa
      241 ccctacaatg tgggctcatt atgctaacaa cagaacaggt atatgtatag aattcgatca
      301 agacaaaata agagaactag aggatatcta tatctttgac gtcaaatata aagatcgccc
      361 tccgactatt gatactgcag tactaacatt aatagctgat caggtgaact ataatcatga
      421 tgcaatttat gatgattatc aagaaaagtt caaacatctt tatgaacaag catttgacga
      481 gggaagaaat ttgcttttgg aaatttacaa tggaatattg gcaactaaat ctcaagaatg
      541 gtcttatgaa caagaaaaac ggctaatata tattttatcc ttctcacaag aagacaaatt
      601 ggcaaaaaaa tataaaactg gcataaacat atcactccct gaaggatgca ttaaaagtgt
      661 tatcctaggt gagaagacaa gtgagcatga taaaaaactt atagtttcag ctataaaaaa
      721 acattctaaa cacattaagt tgaagagagc tacaataaca caaggcgaat atttattatc
      781 tattcgtgat gaagttttta gctgatggta ttataatcga acagatattt tctcctaagc
      841 gttgcattta tcccacggta acatattcat actcaaatat cctgcgacat ggaagatttg
      901 agtatggttg ttcttcatga gcgaaaagca gatgttcgcg ctaaggtata atcaattggt
      961 tgttaaatag tttaaacaca agcaatgact aataaaacaa tgccacaaac cttattattt
     1021 ctaaactagc atttagcttt cagacaattt attgattgtt aatacatatt tttaatattt
     1081 aattatcatt tattcccccc cccctcatgc ttatcaaata cttcacaaca acggaatttt
     1141 aatc
//
LOCUS       DAOTRK010000346         1139 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.350, whole genome shotgun sequence.
ACCESSION   DAOTRK010000346 DAOTRK010000000
VERSION     DAOTRK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.350"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            7..450
                     /locus_tag="QIE41_004848"
     CDS             7..450
                     /locus_tag="QIE41_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDS3533599.1"
                     /translation="MSFASSACFNEAGSIFRIEPNLIKAIALVESNLKNDSIGKNRDK
                     KNNIKSVDYGLMQINQMHIPMLKKRGIIKDERDLLDNPCLNIKIGTEILYNHFSRCGV
                     TWQCLGTYNAGFAMENKKKRLQYAKKIYVVYTRLNEIDKRKALAK"
     gene            complement(469..1101)
                     /locus_tag="QIE41_004849"
     CDS             complement(469..1101)
                     /locus_tag="QIE41_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDS3533600.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGSELIKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          383 a          209 c          188 g          359 t
ORIGIN      
        1 acaaaaatgt ctttcgccag tagcgcttgc tttaatgaag caggatcaat tttcagaatc
       61 gaaccaaatt tgatcaaagc tatcgctctt gttgaaagta atttaaaaaa tgatagcatc
      121 ggcaaaaaca gggataagaa aaacaatata aagagtgttg attatggttt aatgcagata
      181 aaccagatgc acattcccat gttaaaaaaa cgtggaataa taaaagatga acgagatttg
      241 ttagataacc cctgtctgaa cattaaaata gggaccgaga ttttatataa tcatttttca
      301 cgttgtgggg taacgtggca atgtctgggg acatataacg cgggttttgc aatggaaaac
      361 aaaaaaaaga ggctgcaata tgcaaaaaaa atatatgttg tatataccag acttaatgaa
      421 atagacaagc gtaaggcttt agcaaaataa acctaaagcc tctttttttt atatagtgca
      481 aacacccata cgtaaagctg ttttgatcaa ctcgatacca ctgtgaacat ctaatttctt
      541 catgatattg agtctatgag tttcgactgt tttcctgctt aactgcaaga aattagcgat
      601 ttctttgttt gttttcccat cagctatgag ttgtagtatt tcagactcac gatttgtaag
      661 ttgattatca gaggaatatc ttgaagatat tttttcacaa tccatatgta cactatcaaa
      721 ataagtgtaa ccattactaa tcgaatcaat agcgcgattt agtttcgccg gatgactggt
      781 tttccatacg catcctttcg caccggcttc aagcaatttt attgaatcaa tatacggcat
      841 ttttgctaca aatgtaataa tatgagcatc tattttatgc gatttaaccc attttatgag
      901 ttcggacccc atcattccac ctgcaaattc tcctaaaata aatatttggg ctgcagattt
      961 gttacactcc gcgatagctt tcttcagatc tttatagccc ccaacaacct catataaatc
     1021 gggataatgt ccgagaaatc caattatccc atcaatcata aacggctgct gatctgaaac
     1081 tacaatttta atctttccca tatattcatc ctgagtgtaa atgcaataac gcattcatt
//
LOCUS       DAOTRK010000347         1133 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.351, whole genome shotgun sequence.
ACCESSION   DAOTRK010000347 DAOTRK010000000
VERSION     DAOTRK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.351"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..541)
                     /gene="papA"
                     /locus_tag="QIE41_004850"
                     /pseudo
     CDS             complement(1..541)
                     /gene="papA"
                     /locus_tag="QIE41_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
     gene            complement(749..1062)
                     /locus_tag="QIE41_004851"
                     /pseudo
     CDS             complement(749..1062)
                     /locus_tag="QIE41_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
BASE COUNT          332 a          295 c          195 g          311 t
ORIGIN      
        1 gttgcgactg cagaaaaagc accctcagtg acttgggcat tattggctga cgacttctta
       61 acaatcgcag tataatgcag cacattatca ccatctttca gggtattagc atcaccttct
      121 gtgccatcga aagaaacgtt tttacctgca gcctgaacta caatcgcagt acctgtcccc
      181 ccgctggtat ctaactcctc attatgacca gaaacccttg gaccactaaa agccagcttc
      241 acagtccctt tttttgctgc cgagactcct cccccccctt aaatgaagtg atatcacaat
      301 taaccaattc aatatctaaa ttcattggtt tggatacgcc tcctgcttga aggaagcttt
      361 ttgaaagctg tccaaaatca atagactgat cagctgattt ctgagaaatg ctgcatggag
      421 catcaacaac agttccgtta aaggttactc taccctgccc ctgtggagta gttagagcag
      481 catttacacc aaaagacacc actgccatag ctaccgcacc ggcaataacc gacttaatca
      541 taaataacaa cctctttttc attactgaac acaccagaga tatctggcaa tttaaaatga
      601 cccgattacc tttaataacc agatcaatac aaataatatt ctcgtatttt cagggcaggt
      661 tatttatcga cagaatactg atataacctg tctgctttct gacaagggct tcttttttta
      721 tcttccagaa actccggaat gccataattt agtcaaatgc cgatgactca tctgtataat
      781 aaggtgcaag ccttgctgca agagcattca gccgtataag tctccccagt gttgtactga
      841 aatacccatt attcatctgg tatttctcgc agacctcctt acgggagtgc ccacctacca
      901 gatagtcctt catagccaga atgaccctgt cactgtgaat agaagaaata cctatcagta
      961 aaaaaaatgc atttcagaca tagagccgag caacagcacg ctctcgcgta tattcagcaa
     1021 aaacgcattt cctgaccgac tgatgacttc atgatgcgcc atgtttcccc cttctgtcgg
     1081 gcccctggat atatgcttcc ggcccggaaa tactcttcac gcaataagtt aaa
//
LOCUS       DAOTRK010000348         1117 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.352, whole genome shotgun sequence.
ACCESSION   DAOTRK010000348 DAOTRK010000000
VERSION     DAOTRK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.352"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..295
                     /locus_tag="QIE41_004852"
     CDS             <1..295
                     /locus_tag="QIE41_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDS3533601.1"
                     /translation="KTSEASERFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGTNDFEN
                     LVLIENEPYHKVFTNMQSRIAKGVLVGESKITPWAIPSGSIYPPMKNIMEPTK"
     gene            292..786
                     /locus_tag="QIE41_004853"
     CDS             292..786
                     /locus_tag="QIE41_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533602.1"
                     /translation="MMTKTQINKLKKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFNNQPDYLNFLRAMDGFEVNGLRLFSLSIPEPSVKNLFTVNEFYRN
                     NDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIM
                     DSCS"
     gene            881..997
                     /locus_tag="QIE41_004854"
     CDS             881..997
                     /locus_tag="QIE41_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533603.1"
                     /translation="MEKLTTELHSLSEMDRRHVISILAEIANGYDDFSDMLI"
BASE COUNT          416 a          181 c          171 g          349 t
ORIGIN      
        1 aaaaacctcc gaagcatctg aaagattcaa cgccgaagac cttttaagaa tgagaaaggg
       61 caatgttcct ctaaattata atgttcacca taaactatct ctagatgatg gtggtactaa
      121 tgatttcgaa aatttagtat taatcgaaaa cgaaccatat cataaagttt ttactaacat
      181 gcaatcacga atagctaagg gagtattagt aggtgaaagc aaaatcactc cctgggccat
      241 tccatctggc tcaatttatc ctcccatgaa aaatattatg gaacccacaa aatgatgact
      301 aaaacccaaa taaataaatt aaaaaaaatg atgaatgatt tagactatcc atttgaagca
      361 ccgctcaagg aatcatttat tgaaagtata atccaaatag aatttaattc taattcaact
      421 aattgcctgg agaaattatg taatgaagtt agtattcttt ttaataatca acctgattat
      481 cttaattttt taagagcaat ggatggattc gaagttaatg gattacgatt atttagcctc
      541 tcgattccag aaccttcagt taaaaacctt tttaccgtaa atgaatttta tagaaataat
      601 gatgatttca taaaccctga tctacaagaa cggttagtga tcggggatga tagcatttca
      661 atatttactt atgacattaa aagtaacttt tttgaaatta gagataatat tggcaccgaa
      721 aatatattca gttcgtttag tgatttctct tcattcttaa acgaaatcat ggacagttgt
      781 tcataataat caaaagataa caaaaatcat tgtaaaccat gccatctttt tagagattac
      841 cagcagaaaa ttccttgttc gaaacttagc aatgcaagct atggaaaaac ttacaacgga
      901 actacactcc ttaagtgaga tggaccgaag acatgtaatc tctattttag ccgaaatagc
      961 taatggctat gatgatttta gtgacatgtt gatttagtta gaatttcacc cgaatcataa
     1021 gattctgata tcttaaataa gaaaatcccg ttcgacgttc tgaaaaagaa atcacaaaat
     1081 attagcagat acttatttcg tgactgtata ttatccg
//
LOCUS       DAOTRK010000349         1088 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.353, whole genome shotgun sequence.
ACCESSION   DAOTRK010000349 DAOTRK010000000
VERSION     DAOTRK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.353"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            140..>1088
                     /locus_tag="QIE41_004855"
     CDS             140..>1088
                     /locus_tag="QIE41_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDS3533604.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRVGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNIWAGGNAS
                     LTTITTSVIGGNENSKGTVNVLGGTWRLYDSGNNARPLNVGQSGTGMLNIKQKGHVDG
                     GYLRIGSSTGGVGTVNVEGEDSILTTELFEIGSYGTGSLNITDKGYVTSSIVAIVGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEAIIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGKVNISNNGLINNKEY"
BASE COUNT          309 a          197 c          283 g          299 t
ORIGIN      
        1 tagcaaatta gccaaattga caccttccca cctccaacca acactcaata cagacctctg
       61 acaatttaat aagtttcatt tttaatgtct gacgactcat ctctttcgtg gctgtattgt
      121 ttgtatatgg agcgtttata tgaatagaat ctatcgcgtg atatggaatt gcaccctaca
      181 ggtatttcag gcctgctcgg aattgactcg cagggtaggt aaaacatcaa cggttaattt
      241 gcgtaaatcc tctggactga caacgaaatt cagtagattg acgctgggtg ttttgctggc
      301 actaagcggt tcagcgtctg gtgcgagtct ggaagttgat aatgatcaga ttaccaatat
      361 tgatactgat gttgcttatg atgcctacct ggttggctgg tatggcactg gagtgcttaa
      421 tatttgggct ggcggtaatg cctccttaac cactattact accagcgtca ttggtggtaa
      481 tgagaactcg aagggcaccg ttaatgtttt gggtggcacc tggcgattgt atgatagcgg
      541 aaataatgca aggcctttaa atgtgggcca atccggaacg gggatgctga atattaaaca
      601 gaaggggcac gttgacggag gatatttaag gataggttct tcgacaggag gcgtcgggac
      661 ggtcaatgtt gagggagagg actcgattct gacgaccgaa ttattcgaaa tagggagtta
      721 cggtacaggc tcattaaata ttacggataa gggttacgtc acgagttcaa tcgtcgccat
      781 tgtaggatat caggcgaaca gcaatggtaa ggttgtcgtt gaaaagggtg gcgagtggct
      841 aataaaaaat aatgattcct caattgaatt tcaaattggc aatcaaggga cgggggaagc
      901 gattattcgc gagggggggc tgattacggc tgaaaatacg attatcggtg gcaatgccac
      961 cggtatcgga accctgaatg tgcaggatca agactctgtc atcacggtac gcagactcta
     1021 taatggatat ttcggtaatg gcaaagtcaa tatttccaat aatggactga ttaataacaa
     1081 agaatatt
//
LOCUS       DAOTRK010000350         1083 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.354, whole genome shotgun sequence.
ACCESSION   DAOTRK010000350 DAOTRK010000000
VERSION     DAOTRK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.354"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            78..185
                     /locus_tag="QIE41_004856"
     CDS             78..185
                     /locus_tag="QIE41_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533605.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(238..996)
                     /locus_tag="QIE41_004857"
     CDS             complement(238..996)
                     /locus_tag="QIE41_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDS3533606.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          294 a          267 c          257 g          265 t
ORIGIN      
        1 ataacaaaaa atcccttgat gcctgtccct tttgttacac tccgttatca cgcacaagag
       61 atatgcagga cactggtatg ccgactaaac gctttgataa aaaacactgg aagatggtgg
      121 tggtgctact ggcaatctgt ggcgctatgt tgttgctacg ttgggcagca atgatttggg
      181 gctgagcagt gacaaacgaa gacagccaga ccgcataaca cggtccggca gataatttta
      241 catgctgtag cccagcgata aggttgtccg acgatcggta tgttccggtg cagactctgg
      301 aggttcagag ttccatgtta cgttgtaagc caccttcagt ccaaaatgtt cattaatagc
      361 aacatttaat gcgctttcgg agttcagcgt tgtgtcttcc gcgccgaaga ccgaaacacc
      421 ctgcgtaaat ttagcgttgt cagtcaactg ccatgcataa gccccggagg catagcccag
      481 cggctgggtt tcactggcat tgtcggtata tttgtcgtaa cgcacacctg gaccgaattc
      541 aaagcggaaa ctgtgtaccg ggccattcaa aaactgacga ccataacccg cggtcaacac
      601 atcgcgctct cgatagccgt tataacggtc tgtcagccag cttgcctgcc caaataaata
      661 gtcataatca gttaaattaa aacggctacg cccgcccgcg gcatattttt ctgaagaacg
      721 ctcatcatta gaagaagtat tactggcgtt cccccacagc gaccaggcag tggtttgtcc
      781 ataccaggtc atggtggtgt cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc
      841 aagatatcct gcgttcagat taccttcgaa aggtttttta gcgctggcag gatcgtccat
      901 gacagtaaaa acggaatcat cggcagctgc attcaatgac gcaaacatgc cccccgccaa
      961 cataacgatg gcaggaactg tcttcaaaag cttcatttat caagagtccg tacaacaaaa
     1021 aaagagacca tcgcggtccc ggaaactttc ttaaggatca aagattagcg tccctggaaa
     1081 ggt
//
LOCUS       DAOTRK010000351         1079 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.355, whole genome shotgun sequence.
ACCESSION   DAOTRK010000351 DAOTRK010000000
VERSION     DAOTRK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.355"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1079
                     /locus_tag="QIE41_004858"
     CDS             <1..>1079
                     /locus_tag="QIE41_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDS3533607.1"
                     /translation="AVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRD
                     DGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEF
                     GSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLIEG
                     LNFAAQYQGKNERTDNGHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQ
                     ERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEV
                     VAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINL
                     IDESDFTRAVDIRTDNIVATGITYQ"
BASE COUNT          305 a          229 c          253 g          292 t
ORIGIN      
        1 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
       61 acaaggatgg taataaacta gacctttatg gcaaggttac cgctctacgt tattttactg
      121 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
      181 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
      241 atgaagccaa cggctcgcgc ggtaacaaga cccgtctggc ctatgcaggt ttaaaaatta
      301 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctatgac attggttcat
      361 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
      421 cataccgtac taccggtgtg gcaacctatc gcaactacga tttcttcggc ttaattgaag
      481 gtctgaactt tgccgcgcaa tatcaaggca aaaatgaacg cactgacaat ggtcatcttt
      541 atggtgctga ctacacgcgt gccaatggtg acggtttcgg tatctcctca acttatgttt
      601 atgatggctt tggtatcggt gcggtgtata ccaaatccga tcggacaaat gcgcaggaaa
      661 gagccgctgc taatcctctc aatgcctccg gtaagaatgc agaactgtgg gctacaggta
      721 taaaatatga tgccaacaac atctactttg cagctaatta cgctgaaaca ttaaacatga
      781 ccacctatgg cgatggttat atctctaaca aagcacaaag ttttgaagta gtggcacaat
      841 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
      901 tgggccgcta cggcgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
      961 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
     1021 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc aacgggaatt acctatcag
//
LOCUS       DAOTRK010000352         1068 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.356, whole genome shotgun sequence.
ACCESSION   DAOTRK010000352 DAOTRK010000000
VERSION     DAOTRK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.356"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>1068)
                     /gene="rhsC"
                     /locus_tag="QIE41_004859"
     CDS             complement(<1..>1068)
                     /gene="rhsC"
                     /locus_tag="QIE41_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HDS3533608.1"
                     /translation="VAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWVLGWC
                     ERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRL
                     VLTTQAQRAEEARQQASSGGAEPSVFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPEN
                     LPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPDIRYRYDS
                     DGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEGGLKRMVKKEHADGSVT
                     QSQFDAVGGLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSATG
                     PDGLELRREYDESGRLIQETAH"
BASE COUNT          172 a          365 c          311 g          220 t
ORIGIN      
        1 gtgtgcggtt tcctgaatca gacggccgga ttcatcatat tcccggcgca attccagccc
       61 gtcaggcccg gtggctgacg ttaactggtt gtggtggtta tagtaaaatg ccgatgccct
      121 gccatccggt gtggtgatgc gcgtgatgag tcccgtcacc acatccgggc tgtactcagt
      181 cgtccggcct gcggcatccg tctgtgcctt aagcccgccc accgcgtcaa actgactctg
      241 cgtgacgctg ccgtccgcgt gttccttttt caccatccgc ttcagcccgc cttcgccctg
      301 cgtgtgcagc acttcacggc ggtccagact gtcggtgatg gtgatgcggt ctttctcata
      361 ctgatacgtg tagcttaagc ctgccgggtt tagctgttct gtcacccgcc cgtcgctgtc
      421 gtaacggtaa cggatatccg gccggcccgt gtgacggtgc gccaccatcc ggccccggta
      481 tttatcatcg taagtaaagc tgcgcacctg tgtattgctg cggtcataca ccgccgccag
      541 ttcgccgcgt ggcgtccagc catagcgcac cagcggcgcg gcaggtaaat tctccgggta
      601 ttccgggtcg tgcgtcagcc acacggcaga cagacggatg ccgttgtccc ggccatattc
      661 ggtgtaaccc ggcagggtat caggaaaaac ggacggttcc gccccgccgg aagaggcctg
      721 ctgccgggct tcttctgccc gctgcgcctg cgtggtcagt accagccgga agtgacgccc
      781 ggcaccatcc gtcacgccgg tgatttcgcc gctgaattca ccgccagcct cacggtgaaa
      841 cgtctgtgtg cgcccgaagc ggtccaccag cccggtcagt acccggtacg gcggcagcgg
      901 cgcaggcagc acctcatccg cctccggcac ccgctcacac cagccgagta cccaccacgg
      961 cccctgcgga ctgttcgtcg ccagataacg atgcggactc aggcgaagct cttccggcag
     1021 cgcctgccag agtgcggcca gccggtgacc ttcatccagt ttcgccac
//
LOCUS       DAOTRK010000353         1059 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.357, whole genome shotgun sequence.
ACCESSION   DAOTRK010000353 DAOTRK010000000
VERSION     DAOTRK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.357"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(486..>1059)
                     /locus_tag="QIE41_004860"
     CDS             complement(486..>1059)
                     /locus_tag="QIE41_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HDS3533609.1"
                     /translation="LRDIYKDYNNNHEDDNFDKMQKIFLALNEFKERTEINYRDYRKV
                     SKYVDDKVDLFSSIEQYLRWVIEKNNDYNKENYGDIKDKYAKLIVERNDLVDTKDQLI
                     KEDFYLNNKDNKDKKDAERINEIKGIIKVIDGKIPNISKEILHLKDETERLVKEYQQE
                     NTLNDVVQNIRSWLKENENMVKAIKKTDTE"
BASE COUNT          298 a          211 c          137 g          413 t
ORIGIN      
        1 gttttgtgct gtgtagccga aaaatagatg attcagatta actaccgcac tctatgttgt
       61 gtttatatcc atgtcaggta acaggtcatt tgtgaatcct gacacaagac tatgactatc
      121 gcccagggat aatccacaac cgctggaagt agtctcattt ctgcaggata aaaccgtaga
      181 gtgcgattgc gatgaataat aaatacaggc ttattattcc agtctttttt attgcgttgg
      241 ttgattcaaa tggctcggtt agtaaaatgc accattcact cggaaaactc aacatgttgc
      301 actcttatac gccgtctgga aagggcgtag tagtgatata accatttaat cgtctcccaa
      361 catcaacgct tacacatcaa ctacactggt ttaattaaag tacaccagat tactgatgag
      421 taagcgtctt ttcgcatgac ctcattatca caggaacgac ttgatccgtt tatttatatg
      481 ccatgttatt cagtatcagt tttcttaata gctttcacca tattttcatt ttcttttagc
      541 caactcctga tattttgaac tacatcatta agtgtatttt cttgttgata ctctttcacc
      601 aatctctctg tttcatcctt aagatgtaga atctctttac ttatatttgg tatttttcca
      661 tctatcacct ttattatccc cttaatctca ttaattcttt ccgcatcttt tttatcttta
      721 ttgtctttat tatttaagta aaaatcctct ttaattaatt gatccttagt atcgactaaa
      781 tcatttcttt cgacaataag tttggcatat ttgtctttta tatcgccata attttcctta
      841 ttatagtcat tatttttctc aattacccac cgtaaatatt gctcaatgct agagaataaa
      901 tcgactttat catctacata ttttgatact ttcctataat ccctgtaatt tatttccgtt
      961 ctttccttaa attcattcaa tgctaaaaaa atcttttgca ttttatcaaa attatcatct
     1021 tcatgattat tattatagtc tttgtaaata tcccgcaaa
//
LOCUS       DAOTRK010000354         1058 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.358, whole genome shotgun sequence.
ACCESSION   DAOTRK010000354 DAOTRK010000000
VERSION     DAOTRK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.358"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..161)
                     /locus_tag="QIE41_004861"
     CDS             complement(<1..161)
                     /locus_tag="QIE41_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HDS3533610.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFA"
     gene            complement(224..700)
                     /locus_tag="QIE41_004862"
     CDS             complement(224..700)
                     /locus_tag="QIE41_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDS3533611.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(716..>1058)
                     /locus_tag="QIE41_004863"
     CDS             complement(716..>1058)
                     /locus_tag="QIE41_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDS3533612.1"
                     /translation="QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDD
                     DDETWVLFSAMNGNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQH
                     PECSAIMRIID"
BASE COUNT          237 a          279 c          305 g          237 t
ORIGIN      
        1 gcaaacacgg ccagcacacc gggaatatcc tgcacttcac gaccggtata cgcttcagca
       61 ctgccgtgcc agcggtattt accggtacag aacggaaaaa gacgggatgt cggatgttgt
      121 tggtgaatac gcatggcttc accacgggtg ataattttca taatgggata cctctgaaga
      181 cagaagataa aagtgaaaac aggtgtgatg tggttgtgaa gggttaaagc agaccatgtt
      241 cggcaaagga gaaaacctgg ttgccaccga ctatcagatg gtccggcacc cggatatcca
      301 ccaggcccag tgcctgtacc agacgttcgg tgataagccg gtctgcctta ctgggtgtga
      361 cttcaccgga cgggtgattg tgtgccagta ccacggcagc ggcattgtgg tacagggcgc
      421 gtttaatcac ttcccgggga tggacttccg tgcggttgat ggtgccggtg aagagggttt
      481 caccggcaat cagctgattc tggttgttca gatacagcac ccggaattct tcacgctcca
      541 gtcccgccat gttcagaatc agccattccc gtgctgcacg ggtggaggtg aaggccacgc
      601 cgggttcatg aagatggcgg tccagggttt tcagagcccg cagaatgagg ctgcgctcgc
      661 cgggcgtcat ctctccgggc agaaaggaaa gttgttgcat tgtgcttctc tccattcagt
      721 cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc cgcaaccggt
      781 aataatggac cgtcatggca taattctccg tacgacaggc atgatgactg tacgtcatca
      841 gacaggcggc aataccggcg gcttccgggc tcatttctgc gcggttaccg ttcatggcac
      901 tgaacagtac ccatgtttcg tcatcatcgt catccggttc gggtgccata aatgccccgc
      961 cgttgttcag ggtgtacaga ttccagatac caccgcagta gtcttcgcac agacggtcca
     1021 tccagccgaa gacacggggc tccagggtca cccactgt
//
LOCUS       DAOTRK010000355         1057 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.359, whole genome shotgun sequence.
ACCESSION   DAOTRK010000355 DAOTRK010000000
VERSION     DAOTRK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.359"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..516
                     /locus_tag="QIE41_004864"
     CDS             <1..516
                     /locus_tag="QIE41_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDS3533613.1"
                     /translation="AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWA
                     KCTKGVYSSYWEEWKKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGM
                     PVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEA
                     WAERQLKKVTR"
     gene            660..735
                     /locus_tag="QIE41_004865"
     tRNA            660..735
                     /locus_tag="QIE41_004865"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:693..695,aa:Ile,seq:cat)
     gene            743..819
                     /locus_tag="QIE41_004866"
     tRNA            743..819
                     /locus_tag="QIE41_004866"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:777..779,aa:Arg,seq:tcg)
     gene            833..909
                     /locus_tag="QIE41_004867"
     tRNA            833..909
                     /locus_tag="QIE41_004867"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Arg,seq:tct)
BASE COUNT          274 a          212 c          286 g          285 t
ORIGIN      
        1 gcttttgagg attatgcccg cagcgcagcg agtaaaaagc agtgcccttg ttgctatggg
       61 gaaaaattta ttgaaagcgt agtttttaca aacaaggtcc agtatccgga tggtaagccg
      121 ccggtatggg caaagtgtac gaaaggtgtg tattcgtctt actgggaaga atggaaaaaa
      181 gtcagggagg tggtaaaagt tgcctgtccg gagtgtggcg gaaagggtga ggtttccacc
      241 gcctgtaagg attgccgtgg gcgtggtgtc gccattcatc gtgaagagtc ggtaaaacgt
      301 ggtatgcctg ttatcagaga ctgccagcgt tgtggtggtc gtggctatga aagactacca
      361 tcaacggagg catttaatgc tatatgcgag gtgacaaacc agataacacg cgcgtcatgg
      421 gaaaaaacag ttaagaaatt ctatgatgcg ctggtgaccc ggtttgatat tgaagaagca
      481 tgggctgagc ggcagttaaa aaaggtaact aggtaacaag gttgattttt ccggaatctg
      541 tggtaaattc gtcataacaa tgggcttttt atgcctgacg ttagaagagt ttctacaacc
      601 cgccgccgag cgggtttttt attgcggaat taattacgga ccgttattat tctgctcccg
      661 gccctttagc tcagtggtga gagcgagcga ctcataatcg ccaggtcgct ggttcaaatc
      721 cagcaagggc caccatcaca taccgccatt agctcatcag gaaagagcgc cagccttcga
      781 agctggctgc gcggggttca agtccccgat ggcggtccat tatctgcatc atgcgttgtt
      841 agctcagccg gacagagcaa ttgccttcta agcaatcggt cactggttcg aatccagtac
      901 agcgcgccat attcattctt ccagattcct ttcggcagag ccttatactg aaatatacct
      961 ggctcaggat attgttgaaa atattatatg tttgtcaaaa ataaaagttc tgttaagtgt
     1021 tgattgagtg tttgttatac ggtctaatgg ttttttc
//
LOCUS       DAOTRK010000356         1019 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.360, whole genome shotgun sequence.
ACCESSION   DAOTRK010000356 DAOTRK010000000
VERSION     DAOTRK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.360"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1019
                     /locus_tag="QIE41_004868"
     CDS             <1..>1019
                     /locus_tag="QIE41_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDS3533614.1"
                     /translation="AADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDS
                     DDNTGGQQIARGGTARNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQAYGTVLNG
                     GEQWIHAGGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPESENVSSGQMVGGTA
                     ESTTINKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTV
                     INEGGWQVVKAGGTAGNTTINQNGELKVHAGGEASDVTQNTGGALVTSTAATVTGTNR
                     LGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGTATTVSMGNGG
                     VLLADS"
BASE COUNT          250 a          259 c          329 g          181 t
ORIGIN      
        1 gctgctgaca tcgttgtgca cccgggtgaa acagtgaatg gcggaacact ggtaaaccat
       61 gacaaccagt ttgtatccgg aacagctgat ggcgtgactg tcagtaccgg gcttgagctg
      121 gggccggact ctgatgacaa caccggcggt cagcagatag cccggggtgg cactgccaga
      181 aacaccacgg tcaccgcaaa cggtctgcag gatgtcatgg caggtggcag tgccacggat
      241 acggttatca gtgccggtgg aggacagaat ctgcgcgggc aggcttacgg tactgtcctt
      301 aacggtggtg aacaatggat acatgcaggc ggcagtgcat ccggaacggt gattaaccag
      361 tctggctacc agaccattaa acatggcggg caggcaaccg gaaccatcgt caacaccggt
      421 gcagaaggtg gtccggagtc tgaaaatgtg tccagcggtc agatggtcgg agggacggct
      481 gaatccacca ccatcaataa aaatggccgg caggttatct ggtcttcggg gatggcacgg
      541 gacaccctca tttacgccgg cggtgaccag acggtacacg gagaggcaca taacacccgg
      601 ctggagggag gaaaccagta tgtgcacaaa tacggacttg cgctgaacac ggtaattaac
      661 gaggggggct ggcaggttgt taaggcaggt ggtactgccg gtaacaccac cataaatcag
      721 aacggcgagc tgaaggttca tgccggcgga gaagcgtctg atgtcaccca gaacacgggc
      781 ggtgcactgg ttaccagtac agctgcaacc gtcaccggca caaaccgcct gggagcattc
      841 tccgttgtgg cgggtaaggc tgataatgtc gtactggaaa atggcggacg tctggatgtg
      901 ctgagcggac acacagccac taatacccgc gtggatgatg gcggaacgct ggatgtccgc
      961 aacggtggca ccgccaccac cgtatccatg ggaaatggcg gtgtactgct ggccgattc
//
LOCUS       DAOTRK010000357          990 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.361, whole genome shotgun sequence.
ACCESSION   DAOTRK010000357 DAOTRK010000000
VERSION     DAOTRK010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.361"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            12..>990
                     /locus_tag="QIE41_004869"
     CDS             12..>990
                     /locus_tag="QIE41_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HDS3533615.1"
                     /translation="MAAFALCIGYAHAESQTPIRLVIHGGAGTITKDTITPEQEKQYR
                     EKLTDALNAGYAVLNSGGSSLDAVQRAINVMEDSPLFNAGKGAVFTHDGKNELDAAIM
                     DGKTKMAGAVAGVTTIKNPINAAYAVMTKSPHVLMISNGAELFAKEQGLVMVEPAYFK
                     TDFRWQQLQNALKDEKITLDHNGKSASLLLPPKNYDYKYGTVGAVALDKDGNLAAGTS
                     TGGMTNKRYGRVGDSPIIGAGTYADNNTVAVSATGTGEMFIRTSTAYNIAAQVKYKNT
                     PLKEAAQNALDEVKNINGSGGVIVLDKNGNYTMSFNTEGMYRGTIGNDGK"
BASE COUNT          334 a          182 c          220 g          254 t
ORIGIN      
        1 gtataacagt aatggcagcc ttcgcattgt gtataggata tgctcacgct gaatctcaaa
       61 cgccaatacg ccttgtaatt catggtggag ctggcaccat aacaaaagat actattacac
      121 ctgaacaaga aaaacaatac agagaaaagt taacagatgc actaaatgct gggtatgctg
      181 tgcttaatag tggaggaagt agccttgatg ctgttcagcg tgcaattaat gttatggagg
      241 actctcccct cttcaatgct ggaaaaggcg ctgtctttac tcatgatggc aaaaacgaac
      301 ttgatgctgc aattatggat ggaaaaacca aaatggccgg agccgttgca ggagtgacga
      361 ccataaaaaa ccctataaac gctgcttatg cagttatgac taaaagccct catgtattaa
      421 tgatctctaa tggtgcggaa ctatttgcaa aagagcaagg cttagttatg gttgagccag
      481 catatttcaa aactgatttc cgttggcaac agctacagaa cgctttaaaa gacgaaaaaa
      541 taacccttga tcataatggt aaatcagcat ctcttttact ccccccaaag aactacgact
      601 ataaatatgg aactgttgga gcagtcgcgt tggacaaaga tggtaatctg gcagctggaa
      661 catcaacagg aggtatgaca aataaacgtt acggacgcgt aggtgactcg ccaataattg
      721 gagcaggaac ctatgccgat aataataccg ttgctgtttc tgctacaggt actggagaaa
      781 tgtttattag aacttcaact gcatacaata ttgctgctca ggtgaaatat aaaaatacac
      841 cattgaaaga agcagctcaa aatgcgcttg atgaagttaa aaacattaat ggtagtggtg
      901 gtgtcattgt tctagataag aatggtaatt atactatgag tttcaataca gaagggatgt
      961 atcgtggaac tatcggcaac gatggtaaag
//
LOCUS       DAOTRK010000358          976 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.362, whole genome shotgun sequence.
ACCESSION   DAOTRK010000358 DAOTRK010000000
VERSION     DAOTRK010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.362"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1..776
                     /locus_tag="QIE41_004870"
                     /pseudo
     CDS             1..776
                     /locus_tag="QIE41_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          396 a          156 c          165 g          259 t
ORIGIN      
        1 atacagacta tattactatt ggtatcgata accaagaacc aataaaatta cttgcgagct
       61 ataaagacaa agaactcgtc cgtactatca ttaatgagaa catagttgag aaggctcatg
      121 atgatataaa taatgctgaa tttactgatg gtattattgt tagtggtatt gatgatcgta
      181 gtgatattgg cattgactcc aatgatacag acgatattac ttttgatatt gatattgata
      241 ttgaaaatca agaaccaata aaatcaccta cgaacgatga aaaaaaactc ggccaaaccc
      301 tcatcaatga aaatgtagta gagaatactc atgatatcaa taacaagaaa atgatcttca
      361 gcgcaataaa agaaatatat aatggagatc ctgattttat tttcgataaa atatcacgta
      421 acctcataca tacggtaaca aaatccgatg agaacgggat aagcaaacca acggacttat
      481 ttacctggca cggtaaagat aaaaaataca acactctcgc cattgtaatt aaaaatacaa
      541 acggaaacga ttatttatct ctcggttact acgatcagga caactaccat attaaaagag
      601 gaattcgtat taatggtgat agtctcacac aatattgtag tgaaaacgcc aggaatgctt
      661 cagcgtcatt tgaaagcaat aaagctctca tggcagaatt atttgcaaca ggtataaatc
      721 atcaagttgt aaacgaactc aacggggaaa gactgaaaga accaaacgaa gtttttaaac
      781 gttatggtcg agcaataaga tataattttc aagtggacaa tgcaaaatat agacgcaata
      841 atgtaaaaga aatagtttct actttattcg ataacgaagt taacactgac cattctcaaa
      901 atatatatgt gcactataag aaactcgaag gtaaaattga agaaaggctt caaaattgcc
      961 agacggaata tcaaaa
//
LOCUS       DAOTRK010000359          913 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.363, whole genome shotgun sequence.
ACCESSION   DAOTRK010000359 DAOTRK010000000
VERSION     DAOTRK010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.363"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            32..445
                     /locus_tag="QIE41_004871"
     CDS             32..445
                     /locus_tag="QIE41_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533616.1"
                     /translation="MADIVTGLTALKTAFDLAKDIKNATGAYNDAEMRLKFSELYSAL
                     SEAKIELADTQLEMYELKLKIEELQAKLDASDELEYRENVYWRASPINGKPNGPFCPR
                     CYESSEKKLSSMSQVTGHATIAGKYKCNTCNAYVR"
     gene            484..567
                     /locus_tag="QIE41_004872"
                     /pseudo
     CDS             484..567
                     /locus_tag="QIE41_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein DinI"
     gene            complement(646..785)
                     /locus_tag="QIE41_004873"
                     /pseudo
     CDS             complement(646..785)
                     /locus_tag="QIE41_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          286 a          212 c          192 g          223 t
ORIGIN      
        1 tctacacatt gtattttctc aagaggaatt tatggctgac attgtcactg gtttgaccgc
       61 actcaaaacc gcctttgatt tagcaaaaga tataaaaaat gccactggcg catacaatga
      121 tgcagaaatg aggcttaagt ttagtgagct ttatagcgcg ctatctgaag ccaaaataga
      181 actggctgat acacagctag aaatgtatga actaaagctg aagatcgaag agttgcaagc
      241 taagcttgat gcatcggacg agcttgaata tagggaaaac gtatactgga gggcctcacc
      301 tatcaatggc aaaccaaacg gtcctttttg cccaagatgt tatgaaagtt cagagaagaa
      361 gctatcttca atgagtcaag taacggggca tgcaactatt gctggaaaat acaaatgtaa
      421 tacttgtaac gcctacgtta gataaaaagc cgttatgggg ctgggacgga ggctcaaatc
      481 ctctcgtgca aaaattcagc taaaactttt gttcaggaga ctctgaaaga taccaagaag
      541 tctgctgacg agtggtttat ttactaatta gcacgtaaaa tctattatgg ctggaaatca
      601 ttcaatactc gtactatcga aagttcgcca gccgacagca aaacgctatt gcatggcagg
      661 gattgcaggc ctctcgatat tcggtgctaa ggatgtatca acacggttca gcagaacccg
      721 atgcgtcttc aaaaccgcag caacgatatc tcttcctccg ttccatttac agccaccttt
      781 ttcaccctcg attaaccaac aaccagacca gaagacacgc taccaccggc acagcaaaat
      841 ccatcaggct tgccatatcc cacgcgcgtg ggtcaaaacc gccccaccac ggcatattca
      901 ttcgcttgcc atg
//
LOCUS       DAOTRK010000360          904 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.364, whole genome shotgun sequence.
ACCESSION   DAOTRK010000360 DAOTRK010000000
VERSION     DAOTRK010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.364"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            15..260
                     /locus_tag="QIE41_004874"
     CDS             15..260
                     /locus_tag="QIE41_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533617.1"
                     /translation="MALELSYVYIKYIYGKEKAEFQKPYSITDDNNCWKIEGKQPKTL
                     GGNFTILIAKKDGQALDVIHKIIHCSDDSNILPRCVS"
BASE COUNT          316 a          146 c          186 g          256 t
ORIGIN      
        1 taaatagtcg tgaaatggct ttggaattat catatgttta tattaaatat atatacggga
       61 aagaaaaagc agaatttcag aaaccatata gtattacaga tgacaataat tgttggaaaa
      121 tcgagggaaa acaaccgaag acgttaggag gaaattttac aatactaata gcgaaaaaag
      181 atgggcaagc gttggatgta atacacaaaa taatccactg ttcagacgat tcaaatattt
      241 taccaaggtg tgtttcataa ctattttgtg tacaatatga ctctacatat cgacgaaaaa
      301 accacttgat ttgacaggaa cacattcaaa aaaatttctt tttcatttca ggagcgattt
      361 tcatctgtta cttgaagtat ctgtgagatt aagtgactcc ccctaacgtc gtactaaata
      421 gtaattactg gccataccgg gagcagtatt cggcgcttcc cacatgcagc agaataaccg
      481 catcagcgaa gatgtattgt atttttacta tcacaatgtg tacggacggt ttatggaaaa
      541 gaatgaacat tagatacgcg atgcggcggc agattacacg gttatcacta cgatagccaa
      601 tactggctgg tgcattaccg ccgtgaacaa taggacgccg tactgctgga aatttcttac
      661 ctttatgact ccccgcaccg ccgtatcggg aaacgggaag cagtcaagat gaggctgata
      721 taatgtgtgg aatgcctgag tatttttaga atagagggta atatgtttga tagaatatat
      781 cttggtgatc gaccaataaa aaaattgaat ttgatttatg gagcaaacaa attagaattc
      841 aggtgaattt aatctcaagg atagcctatg gtacaacaga atggaatttt tataataatg
      901 aaga
//
LOCUS       DAOTRK010000361          895 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.365, whole genome shotgun sequence.
ACCESSION   DAOTRK010000361 DAOTRK010000000
VERSION     DAOTRK010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.365"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..849
                     /locus_tag="QIE41_004875"
     CDS             <1..849
                     /locus_tag="QIE41_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDS3533618.1"
                     /translation="EESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDHRGLPLA
                     LVSTEGTTAWYAEYDEWGNQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQ
                     GRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLYEFKSKNIDDIGIFALAMCNGESIN
                     ENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKG
                     NKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNLKAKGNGVTI
                     IRQGSN"
BASE COUNT          298 a          164 c          216 g          217 t
ORIGIN      
        1 gaggaaagcc gccgctggct ggcatcgtgc ggcctgacgg tggagcagat acaaaaccag
       61 atggacccgg tgtacacgcc ggcgcgaaaa atccacctgt accactgcga ccatcgcggc
      121 ctgccgctgg cgcttgtcag cacggaaggg acaacagcgt ggtacgcaga atacgatgaa
      181 tggggtaacc agctgaatga agagaacccg catcagctgc agcagctcat ccgcctgccg
      241 ggtcagcagt atgatgagga gtccggcctg tattacaacc gccaccgcta ttatgacccg
      301 ctgcaggggc ggtatatcac tcaggatccg attgggctga aggggggatg gaatttttat
      361 cagtatccgt tgaatccgat ctcaaatata gatccattag gattatatga atttaaatca
      421 aaaaatatag atgatattgg aatatttgca ttggcaatgt gtaatggaga atcaattaac
      481 gagaataaag aatatggtgg gctaatatgt aagaagcaag gtgaatattt ccctatgaat
      541 ccgataagtt caaatgataa tgatagtgta gacttgcgaa atataaaatg ccctgaaggt
      601 tcagagagag taggcgatta tcacactcac ggtttttact ctgacgataa aggcaataaa
      661 gtaacaaaag aaaatgatgt ttatgatagt ctaaattttt caagcaaaga tttaacgaat
      721 tcttatatga atggaatggg aaaaaaagaa tacagtagtt acttgggaac accaaataac
      781 acctatctaa aatataatct caaagctaaa gggaatggag ttacaattat caggcaaggg
      841 agtaattaaa atgaaggtaa tacaatgttg ttatttcctt gctttagtct ccagt
//
LOCUS       DAOTRK010000362          893 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.366, whole genome shotgun sequence.
ACCESSION   DAOTRK010000362 DAOTRK010000000
VERSION     DAOTRK010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.366"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            150..713
                     /gene="fimA"
                     /locus_tag="QIE41_004876"
     CDS             150..713
                     /gene="fimA"
                     /locus_tag="QIE41_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDS3533619.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          249 a          171 c          202 g          271 t
ORIGIN      
        1 atcgttcttc gtgaaatgta ataaccctgg aaactatatt ctcagggcaa tatcccatca
       61 agaatgattc ttgttttata agcaataaca gaatgactta attgttaact ttctttattc
      121 agctacttaa atataaattt tggagaatta tgaaactcaa acatgtcggt atgattgtcg
      181 tttctgtgtt ggcgatgtcg tctgctgcgg taagcgcagc cgagggtgat gaatcagtaa
      241 cgaccactgt taatggcggt gttattcatt ttaaaggtga agtggtaaat gccgcttgtg
      301 cgattgattc agaatcaatg aaccaaacgg ttgagctggg tcaggtccgt tcttctcgtc
      361 tggctaaagc gggtgacctc agctccgccg ttggcttcaa tatcaagctg aatgattgtg
      421 ataccaatgt ttccagtaat gcagctgttg cattcctggg tactactgtc accagtaatg
      481 acgatacgtt agcgctgcag agttcagcgg caggctctgc ccaaaatgtc ggtattcaaa
      541 ttttggaccg tacgggtgag gtattaatac ttgatggtgc cacttttagc gctaaaactg
      601 acttgattga tggcacgaat atactgccgt tccaggctcg ttatattgct ctcgggcagt
      661 cagtagctgg tactgcaaac gctgatgcga ccttcaaagt tcaatatcta taattcaatc
      721 ttaaggtggc tatacgtcat ctgaaattga aacagggtgt gtgtggcatg gctacgcaca
      781 cccgagcaat tcaccaatgt tcatctcatt attaatgagg aatagtttgt gttttggagc
      841 ggtatgaaaa aatcacataa tatctaaacg atcaataaac aacagtagtt aaa
//
LOCUS       DAOTRK010000363          882 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.367, whole genome shotgun sequence.
ACCESSION   DAOTRK010000363 DAOTRK010000000
VERSION     DAOTRK010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.367"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>882
                     /locus_tag="QIE41_004877"
     rRNA            <1..>882
                     /locus_tag="QIE41_004877"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          205 a          201 c          279 g          197 t
ORIGIN      
        1 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
       61 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
      121 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
      181 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
      241 aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta atgtttgatg
      301 ttctaacgtt ggcccgttat ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg
      361 cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg gtcggacatc
      421 aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg cgagcaggtg
      481 cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct caacggataa
      541 aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac ggcggtgttt
      601 ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag ggtatggctg
      661 ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag ttcggtccct
      721 atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag gaccggagtg
      781 gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc taaatgcgga
      841 agagataagt gctgaaagca tctaagcacg aaacttgccc cg
//
LOCUS       DAOTRK010000364          881 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.368, whole genome shotgun sequence.
ACCESSION   DAOTRK010000364 DAOTRK010000000
VERSION     DAOTRK010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.368"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            239..835
                     /gene="fimE"
                     /locus_tag="QIE41_004878"
     CDS             239..835
                     /gene="fimE"
                     /locus_tag="QIE41_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDS3533620.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          248 a          161 c          200 g          272 t
ORIGIN      
        1 attttttaag caactggaag ttaatcttct gcaatctatt gttatattga atcaaatcaa
       61 tgaaaataga tgttgtcaca tcggtgatat tttatttttg tatgatattt aatgtaattg
      121 actgatagcc acatcacttc gtgtgtggtt atctttttat ctattgggct aattttgacc
      181 gattgaggtt tcctataggt attcattcaa atatatctca gtcaggagta ctactattgt
      241 gagtaaacgt cgttatctta ccggtaaaga agttcaggcc atgatgcagg cggtttgtta
      301 cggggcaacg ggagccagag attattgtct tattctgttg gcatatcggc atgggatgcg
      361 tattagtgaa ctgcttgatc tgcattatca ggatcttgac cttaatgaag gtcgaataaa
      421 tattcgccga ctgaagaacg gattttctac cgttcacccg ttacgttttg atgagcgtga
      481 agccgtggaa cgctggaccc aggaacgtgc taactggaaa ggcgctgacc ggaccgacgc
      541 tatatttatt tctcgccgcg ggagtcggct ttctcgccag caggcctatc gcattattcg
      601 cgatgcaggt attgaagctg gaaccgtaac gcagactcat cctcatatgt taaggcatgc
      661 ttgcggttat gaactggcgg agcgtggtgc cgatacccgt ttaattcagg attatctcgg
      721 gcatcgaaat attcgccata ctgtacgtta taccgccagt aatgctgctc gttttgccgg
      781 attatgggaa agaaataatc tcataaacga aaaattaaaa agagaagagg tttgatttaa
      841 ctcattgata ataaagttaa aaaacaaata aatacaagac a
//
LOCUS       DAOTRK010000365          880 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.369, whole genome shotgun sequence.
ACCESSION   DAOTRK010000365 DAOTRK010000000
VERSION     DAOTRK010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.369"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>880
                     /locus_tag="QIE41_004879"
     CDS             <1..>880
                     /locus_tag="QIE41_004879"
                     /inference="COORDINATES: protein motif:HMM:NF036385.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HDS3533621.1"
                     /translation="QKMMSECKYHEGNAFVIMGAGEQLKRIKYDANENKLKVYNVHFD
                     NNQELVADGEPDVVFLKKTVWEDLLVKLKLENKENAVSEINLSSNKNNVDQFIECAKR
                     NEQTLFGNIRKSDFHVASLQPGRTRSVISETPPNDCMESHNLYENHTDTVSTVTKNSQ
                     QVKGHYGDKLKEMQLFLNQMSNALQQDSSLLESKEHTIDIQEKTNKFVQHFQRVLFDK
                     NGRSSEFLLNFYECCYKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNVGFNFKLF
                     PEVKLSGGELETVFKYK"
BASE COUNT          321 a          135 c          167 g          257 t
ORIGIN      
        1 caaaaaatga tgtctgaatg taaatatcat gaaggcaatg cttttgtcat tatgggagcc
       61 ggagaacaat taaaacgtat taaatatgat gctaatgaaa ataaattaaa agtatacaac
      121 gtacactttg ataataatca agagttagtt gctgatggtg agcctgacgt agtattttta
      181 aaaaaaacgg tgtgggaaga ccttctcgtt aaactaaagc tggagaacaa agaaaatgcg
      241 gtttctgaaa ttaacctgtc atctaataaa aataatgttg atcagtttat tgaatgcgct
      301 aagagaaatg aacagaccct attcggcaat ataagaaaaa gtgattttca tgttgcttca
      361 cttcagccag gtagaacgcg tagtgttatt tcagaaacgc cgccaaatga ctgtatggaa
      421 tcacataatt tatatgaaaa ccacacagat acggtttcaa ctgtaactaa aaattctcag
      481 caagttaaag gtcactatgg agataagttg aaagaaatgc agttgttcct caaccagatg
      541 agcaatgcac ttcaacagga ttcatctttg ttagagtcaa aggaacacac catagatatt
      601 caagaaaaaa cgaataagtt tgtgcagcat tttcagcggg tattatttga taaaaatgga
      661 aggtcatcag agtttctact taatttttat gagtgttgct ataagttttt accaagagcg
      721 cagcctcaag ataaaatcga tagctataat tcagcactgc aagctttttc catcttttgt
      781 tcatctacgt tgacacataa taatgtaggg tttaatttca aattatttcc agaagtcaag
      841 ctgtctggag gagagcttga aacggtattc aaatacaaaa
//
LOCUS       DAOTRK010000366          876 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.370, whole genome shotgun sequence.
ACCESSION   DAOTRK010000366 DAOTRK010000000
VERSION     DAOTRK010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.370"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>876
                     /locus_tag="QIE41_004880"
     CDS             <1..>876
                     /locus_tag="QIE41_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533622.1"
                     /translation="GPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASRLIRRG
                     HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQT
                     AAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTGAGNIS
                     ALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVA
                     GGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWL
                     AGSFLLTGHEIENLN"
BASE COUNT          188 a          226 c          266 g          196 t
ORIGIN      
        1 atggccccat gcaactggaa gtcccccgtg atcagtatgc cggcgctgtt gaaaccatga
       61 ggaataagat ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc
      121 tgattcgtcg gggacatctg acttataccc aggcccgtaa tatcacccgg ttcgggacca
      181 tcgaatcggt cacttatgat attgccgagg gttcggttgt cagtctggcg gccggaggga
      241 tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc
      301 tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca
      361 caacccagca actccaccgg ctcagtgttg ttcagggtat gctgaagcac attgattttt
      421 cgactgccag cccgactgtc cgactggctc ttcagaaggg gaccggtgca ggaaatatca
      481 gtgccctgaa caaagtaatg aagggtacgc tggtgacatc tctggcactg gtagctgtca
      541 caaccggccc tgacatgatc aaaatgttgc ggggacggat ctccggtgcg cagttcatca
      601 ggaatcttgc cgtggcatct tccggtgtgg caggtggagc tgtcgggtca gtggcgggcg
      661 ggatactgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg
      721 ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag
      781 aagatcgcgt caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt
      841 tcctgctgac cggacatgag attgaaaatc tgaacg
//
LOCUS       DAOTRK010000367          876 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.371, whole genome shotgun sequence.
ACCESSION   DAOTRK010000367 DAOTRK010000000
VERSION     DAOTRK010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.371"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>876
                     /locus_tag="QIE41_004881"
     CDS             <1..>876
                     /locus_tag="QIE41_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533623.1"
                     /translation="GPMQLEVPRDQYAGAVETMKNKIREGKVPGVTDPAEASRLIRRG
                     HLTYTQARNITRFGTIESVTYDIAEGSVISLAAGGISFALTASVFWLSTGDRDAALQT
                     AAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPTVRQALQKGTGAGNIS
                     ALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVA
                     GGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWL
                     AGSFLLTGHEIENLN"
BASE COUNT          188 a          224 c          269 g          195 t
ORIGIN      
        1 atggtcccat gcaactggaa gtcccccgtg atcagtatgc cggcgctgtg gaaaccatga
       61 agaataagat ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc
      121 tgattcgtcg gggacatctg acttataccc aggcccgtaa tatcacccgg ttcgggacca
      181 tcgaatcggt cacttatgat attgccgagg ggtcggttat cagtctggcg gccggaggga
      241 tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc
      301 tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca
      361 caacccagca acttcaccgg ctcactgttg ttcagggtat gctgaagcat attgattttt
      421 cgacggccag cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca ggaaatatca
      481 gtgccctgaa caaagtgatg aagggtacgc tggtgacatc tctggcactg gtagctgtca
      541 caaccggccc tgacatgatc aaaatgttgc ggggacggat ctccggtgcg cagttcatca
      601 ggaatcttgc cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg
      661 ggatactgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg
      721 ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag
      781 aagatcgcgt caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt
      841 tcctgctgac cggacatgag attgaaaatc tgaacg
//
LOCUS       DAOTRK010000368          876 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.372, whole genome shotgun sequence.
ACCESSION   DAOTRK010000368 DAOTRK010000000
VERSION     DAOTRK010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.372"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..497
                     /locus_tag="QIE41_004882"
     CDS             <1..497
                     /locus_tag="QIE41_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDS3533624.1"
                     /translation="TSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGA
                     YEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRRE
                     DYGKDLWSTYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETML
                     ESLR"
     gene            761..>876
                     /locus_tag="QIE41_004883"
     CDS             761..>876
                     /locus_tag="QIE41_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDS3533625.1"
                     /translation="MKTLSQNTTASACAPETDLQQLVATLVPDEQRISFWPQ"
BASE COUNT          200 a          245 c          246 g          185 t
ORIGIN      
        1 gtacctccag ctaccagatg ctgccgggtt acttcaggtt cgtctgccag aacggatgcg
       61 tctgtggtca gtctctgggg gaagtgcgtg ttccgcaccg gggaaatgtg gtggacagag
      121 tcattgaagg ggcttacgag gtggtgggcg tgtttgaccg gatagaggag aagcgtgatg
      181 ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc gctggcacag gcggcactga
      241 cttaccgtta tggtgacgaa catcagcccg tcaccaccgc cgacattctg acgccacgac
      301 gccgggagga ttacggtaag gacctgtgga gtacttatca gaccatccag gagaatatgc
      361 tgaaaggcgg gatttccggt cgcagtgcaa aaggaaaacg tatccatacc cgggccattc
      421 acagcattga taccgacatt aagctcaacc gtgcattgtg ggtgatggca gaaacgatgc
      481 tggagagcct gcgctgatgc aatctgtccc tgaaggcgtg attcaggcac cagaatcacc
      541 cgacgccccg gagagctcag tctgtgcaga ctgagtacag gcaatggcat ctggccatcc
      601 gtctgtgacc ggaacggtta cgcgtacggc tgtccggaat ttcagccgaa tatcattcat
      661 ccctgacatt accgggtcat cacggtcagt gatgaacgtg atgttgccag ctcccgacct
      721 ttactccctc tcatttactc acattacaag gatttcatac atgaaaacat tgtctcagaa
      781 caccaccgct tcggcctgtg caccggagac tgacctgcaa caactggttg ccaccctcgt
      841 ccctgatgaa cagcgcatca gcttctggcc gcagca
//
LOCUS       DAOTRK010000369          862 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.373, whole genome shotgun sequence.
ACCESSION   DAOTRK010000369 DAOTRK010000000
VERSION     DAOTRK010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.373"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..851)
                     /locus_tag="QIE41_004884"
     CDS             complement(<1..851)
                     /locus_tag="QIE41_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3533626.1"
                     /translation="MDIPVNAAKPGRRRYLTLVMIFITVVICYVDRANLAVAAAHIQE
                     EFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIAIFGWSVATLFQGFAT
                     GLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFYTSGQFVGLAFLTPLL
                     IWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKAELDYIRDGGGLVDGD
                     APVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVTSTLWFFLTWFPNYLTQEKGITAL
                     KAGFMTSV"
BASE COUNT          250 a          243 c          196 g          173 t
ORIGIN      
        1 accgaggtca taaagcctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc
       61 gggaaccagg tcaaaaaaaa ccacagtgta gaagtcaccg caaattgtcc aagatagacg
      121 ccgatcagtt tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgtgcc
      181 tcttttttca ccggcgcatc accatccacc agaccgccgc catcacgaat gtaatccagt
      241 tcagctttgc tgatgccttt ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc
      301 gaccagataa tgccgatacc accggtgaca atgaacaccc agtgccagct caacatctcc
      361 tgaatccaga tcagcagcgg agtcagaaac gccagaccga caaactgacc agacgtataa
      421 aaaccaacgg cagaagcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg
      481 gtcgggaaag cgggcgcttc gaaaataccg gttatcgcgc gcagaccaat taatgacatt
      541 aatcccgtgg caaagccctg gaataacgtc gccactgacc agccaaatat cgcaataaaa
      601 taagtcacgc gtgaacctac gcgatctaaa aaccaaccgc cggggatctg acatagcgta
      661 taaagccagg cgaaggccga aaatacatag cccatttccg ctttggtaat gccgaactct
      721 tcctgaatat gggcggcagc cacggccagg ttagcgcggt caacataaca aatgaccacc
      781 gtaataaaga tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg
      841 ggaatatcca tagcgagctt tc
//
LOCUS       DAOTRK010000370          849 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.374, whole genome shotgun sequence.
ACCESSION   DAOTRK010000370 DAOTRK010000000
VERSION     DAOTRK010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.374"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..805
                     /locus_tag="QIE41_004885"
     CDS             <1..805
                     /locus_tag="QIE41_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDS3533627.1"
                     /translation="DYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRV
                     LSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNI
                     PEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNIAFNMNN
                     IYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFN
                     IAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          278 a          156 c          154 g          261 t
ORIGIN      
        1 tgattattta aagtcattta atgaaacagc aaaacaattt aatacctcaa ttattgtata
       61 tttaattgac cccaaatact ttgctgatct gccgacgtca cagttttggt cgtacgcgac
      121 atacttcagg gtattgtctt ttgaatatct gagtgaaagt atttccacac tgctgtatct
      181 ggatgccgat gttgtttgta aaggaagcct gaaacctctc acagaaatta tatttaaaga
      241 tgagtttgct gcggtcattc ctgacaatga tagtactcag gcggcatgtg caaaacgcct
      301 caacattccc gaaatgaatg gacgttattt caatgcaggc gttatctatg tcaatcttaa
      361 aaaatggcat gaagcaaatt tgacaccgta tttactcaaa cttttacgag gggaaactaa
      421 atatggctct cttaaatatt tagatcagga tgcgttgaat atcgcattta atatgaataa
      481 tatctacctt gcgaaggatt ttgatactat ttataccctg aaaaacgaac tttatgatcg
      541 tagtcatcga aagtatcagc aaaccattac cgataaaaca gtgttgattc actatacagg
      601 gataactaaa ccatggcata gctgggctgg atatccgtct gcatcatact ttaatatcgc
      661 gcgtgaacaa tctccctgga agaaatatcc tcttaaagag gcgcggactg ttgcagaaat
      721 gcagaaacaa tataagcatc tgtttgccca tggtgagtat ataaaaggca taacttcatt
      781 aattaagtac aagcttaaga aataaattac tcttatgatt acaagtatac gttatcgcgg
      841 cttctcatt
//
LOCUS       DAOTRK010000371          808 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.375, whole genome shotgun sequence.
ACCESSION   DAOTRK010000371 DAOTRK010000000
VERSION     DAOTRK010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.375"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..161)
                     /locus_tag="QIE41_004886"
     CDS             complement(<1..161)
                     /locus_tag="QIE41_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HDS3533628.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFSFCTGKYRWHGSAEVYTGREVQ
                     DIPGVLAVFA"
     gene            complement(224..700)
                     /locus_tag="QIE41_004887"
     CDS             complement(224..700)
                     /locus_tag="QIE41_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDS3533629.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVPFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADCLITERLVQALGRVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(716..>808)
                     /locus_tag="QIE41_004888"
     CDS             complement(716..>808)
                     /locus_tag="QIE41_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDS3533630.1"
                     /translation="ECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT          186 a          209 c          239 g          174 t
ORIGIN      
        1 gcaaacacgg ccagcacacc gggaatatcc tgcacttcac gaccggtata aacctcagca
       61 ctgccgtgcc agcggtattt gccggtacag aacgaaaaaa gacgggatgc cggatgctga
      121 cggtgaatac gcatggcttc gccacgggtg ataattttca taatgggata cctctgaaga
      181 cagaagataa aagtgaaaac aggtgtgatg tggttgtgac gggttaaagc agaccatgtt
      241 cggcaaagga gaaaacctgg ttgccaccga ctatcagatg gtccggcacc cggatatcca
      301 cccggcccag tgcctgtacc agacgttccg tgataaggca gtctgccttg ctgggtgtga
      361 cttcaccgga cgggtgattg tgtgccagca ccacggcagc ggcattgtga tacagggcgc
      421 gtttaatcac ttcccgggga tggacttccg tgcggttgat ggtgccggtg aagagggttt
      481 caccggcaat cagctgattc tggttattca gatacagcac ccggaattct tcacgctcca
      541 gtcccgccat gttcagaatc agccattccc gtgccgcacg ggtggaggtg aagggcacgc
      601 cgggttcatg aagatggcgg tccagggttt tcagggcccg cagaatgagg ctgcgctcgc
      661 cgggcgtcat ctctcctggc agaaaggaaa gttgttgcat tgtgcttctc tccattcagt
      721 cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc cgcagccggt
      781 aataatggac cgtcatggca taacactc
//
LOCUS       DAOTRK010000372          792 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.376, whole genome shotgun sequence.
ACCESSION   DAOTRK010000372 DAOTRK010000000
VERSION     DAOTRK010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.376"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            142..>792
                     /gene="mcbR"
                     /locus_tag="QIE41_004889"
     CDS             142..>792
                     /gene="mcbR"
                     /locus_tag="QIE41_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDS3533631.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTSQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQ"
BASE COUNT          246 a          157 c          184 g          205 t
ORIGIN      
        1 ctttaattaa ctaactacgt cattgttctg ctgctaataa caataaaggt gagtggttta
       61 tgattattca ctttaatacc ccaggtgaac tccttctgcc atgcaggcag ggttggacag
      121 aaaacacgcg aaggaattaa aatgcctgga acggaaaaaa tgaaacatgt cagtttgact
      181 ctgcaggttg agaatgacct gaaacatcag cttagtattg gcgcactaaa acctggcgca
      241 cgcctgatta ctaaaaatct ggcggagcaa ttaggtatga gtattacacc tgtgcgtgaa
      301 gcattattac gtctggtttc ggtgaatgcg ctttctgtcg caccagcgca agcatttaca
      361 gttccggaag tggggaaacg tcaattagac gaaatcaatc ggatccgcta cgagctggaa
      421 ttaatggcag ttactctggc tgttgaaaac ctcacctcgc aagacctggc ggaactcgag
      481 gaattgctcg agaaattaca gcaggcgcaa gaaaagggcg acatggaaca aatcattaat
      541 gcgaacaggc tatttcgctt agcaatttat catcgttcaa atatgcccat cctgtgtgag
      601 atgattgagc aactgtgggt caggatggga cctggtttac attatcttta tgaagcgatt
      661 aatccagcgg aattacggga acgtatagaa aactatcgtc tattactcgc cgcgttaaaa
      721 gcaaaagaca aagagggatg cagacattgt cttgctgaaa ttatgcaaca aaatattgct
      781 attttatacc ag
//
LOCUS       DAOTRK010000373          785 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.378, whole genome shotgun sequence.
ACCESSION   DAOTRK010000373 DAOTRK010000000
VERSION     DAOTRK010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.378"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            23..745
                     /gene="sdiA"
                     /locus_tag="QIE41_004890"
     CDS             23..745
                     /gene="sdiA"
                     /locus_tag="QIE41_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDS3533632.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT          212 a          171 c          198 g          204 t
ORIGIN      
        1 ctcaggggcg ttgcggttta ctatgcagga tacggatttt ttcagctggc gtcgcacgat
       61 gctgttgcgt tttcagagga tggaggccgc agaagaggtc taccatgaaa tagagcttca
      121 ggctcagcag ctggagtacg attactattc gttatgtgtt cgccacccgg taccattcac
      181 gcgacctaaa gtggctttct acaccaatta ccctgaggct tgggttagtt attatcaggc
      241 aaaaaacttt ctcgcaattg atcctgtgct gaaccctgaa aactttagtc agggccattt
      301 aatgtggaat gatgacttat tcagcgaagc acagccgtta tgggaagccg cgcgcgcaca
      361 tggtttacgc cgcggtgtca ctcagtattt aatgctgcca aatcgggcgc tgggcttttt
      421 gtccttttcc cgttgcagca cgcgcgaaat acccattctt agtgatgaac tgcaattaaa
      481 aatgcagtta ctggtgcgag aaagtctgat ggctctgatg cgtttaaatg atgaaatagt
      541 gatgacgcca gagatgaatt tcagcaagcg cgaaaaagaa attctgaaat ggacggcgga
      601 agggaagaca tcagcagaga tagcgatgat tttgtcaatc tctgagaata cggtcaattt
      661 ccatcagaaa aacatgcaga aaaaaatcaa tgcaccaaat aagacccagg tggcctgtta
      721 cgcggccgct actggcttaa tttgatcgct tttctttctg cgtgccagat gcaaaaaccg
      781 gctga
//
LOCUS       DAOTRK010000374          772 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.379, whole genome shotgun sequence.
ACCESSION   DAOTRK010000374 DAOTRK010000000
VERSION     DAOTRK010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.379"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            13..>772
                     /locus_tag="QIE41_004891"
     CDS             13..>772
                     /locus_tag="QIE41_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533633.1"
                     /translation="MNSKKVCCIYVLFSLLAGCVSESLIDEKKKKAQTTQSNFNKNAP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDTALKSTVWSDYKNEKLTNTISLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPFPGKNEASTTDMTEQQIIQLKQKTYKAMV"
BASE COUNT          259 a          154 c          168 g          191 t
ORIGIN      
        1 gagtgtccga ttttgaattc aaaaaaggtt tgttgtatat atgtattatt ctcgctgctt
       61 gcaggatgtg tctctgaatc tcttattgat gaaaagaaga aaaaagctca aaccacacaa
      121 agtaatttta ataaaaacgc tccgcagcaa ctaacagaca aagacttatt tggtaatgaa
      181 accacactag ccgtatccga ggaggatatt caagctgcgc ttgatggaga tgagttccgc
      241 gttcccctta attcacctgt aatacttgtt caatccggca accgcgcacc ggaaaccatt
      301 atgcaggagg agatgcgaaa atattatact gtttccacat tctctggtat cccggacaga
      361 caaaagcctc tgacttgtaa caaaaacaaa gataaaaatg agaacgagga tgttgccagt
      421 gctgaaaata tgaactggat gcaggcatta cgttttgtgg cggcaaaagg ccatcagaaa
      481 gcgattattg tttaccagga tacgttgcag acaggaaaat atgacactgc actgaaatca
      541 acagtatggt ccgattataa aaatgaaaaa ctgactaaca caatctcact gcgctatctg
      601 gtgcgattca cgctggtgga tgtagctaca ggtgagtggg ctgcctggtc gccggtgaat
      661 tatgaatata aagtgcttcc accatttccc ggcaagaatg aagccagtac gactgatatg
      721 acagagcagc aaatcataca acttaagcaa aagacctata aagcgatggt aa
//
LOCUS       DAOTRK010000375          767 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.380, whole genome shotgun sequence.
ACCESSION   DAOTRK010000375 DAOTRK010000000
VERSION     DAOTRK010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.380"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            42..758
                     /locus_tag="QIE41_004892"
     CDS             42..758
                     /locus_tag="QIE41_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDS3533634.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          255 a          129 c          166 g          217 t
ORIGIN      
        1 tttaccagta atgtaaataa attttcgagg agatcattcc agtgggacgt aaatgggcca
       61 atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc taaaatttat gcaaaattcg
      121 gtgtagaaat ctatgctgct gccaaacaag gtgaacccga tccagaatta aacacatctt
      181 taaaattcgt tattgaacgt gcaaagcagg cacaagttcc aaagcacgtt attgataaag
      241 caattgataa agccaaaggc ggtggagatg aaacgttcgt acagggacgt tatgaaggct
      301 ttggtcctaa tggctcaatg attattgctg agacattgac ttcaaatgtt aaccgtacga
      361 ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa tatcggagcg gcaggttctg
      421 tcagctatat gtttgacaat acgggtgtga ttgtatttaa aggaacagac cctgaccata
      481 tttttgaaat tttgcttgaa gctgaagttg atgttcgtga tgtaactgaa gaagaaggta
      541 atattgttat ttatactgaa cctactgacc ttcataaagg aatcgcggcg ctaaaagcag
      601 ctggaatcac tgagttctca acaacagaat tagaaatgat tgctcaatct gaagttgagc
      661 tttccccaga agatttagaa atctttgaag ggcttgttga tgcccttgaa gatgacgacg
      721 atgtacaaaa agtttatcat aacgtcgcaa atctctaatt atatgtt
//
LOCUS       DAOTRK010000376          760 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.381, whole genome shotgun sequence.
ACCESSION   DAOTRK010000376 DAOTRK010000000
VERSION     DAOTRK010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.381"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            411..>760
                     /locus_tag="QIE41_004893"
     CDS             411..>760
                     /locus_tag="QIE41_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDS3533635.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKA"
BASE COUNT          191 a          160 c          167 g          242 t
ORIGIN      
        1 tttgttattt ataaaaatgt catcctgtcc atagaatatt gtactcagca ttcagtgcag
       61 gaactgtcat cttctttatt ttattctact tcaggagggc agttttctga aaacgttact
      121 ccggggaaac tgtttatgtt tattcccggc aggaaacccg tacctgcaat accctggatg
      181 atgctttttg ctggtacgtt gttgtgtgaa aattaatcaa catgtgtgtt ccggagagcg
      241 cccgtagacg cacagattca ttctaacatt ctttagcgac aaaataccga atggttatta
      301 tttaatctga aaatcgacag aaatttttat cctgtattga ttctgctgct atttttgcta
      361 ttagtagcaa tagttgcggt actttatgtt ctgtgtgttg tgaggtcatt atgccatact
      421 ttcagtatcc tgatgagttt cccctgagct cgctgcctcc actcattcgt gatgccgtta
      481 ttgaagctca gcaaatcacg caggctcccc tggggcttgt ggcagcatct gccctgggcg
      541 ctgtttcact tgtctgccag aatctgattg atgtctgcag actgaataca ctgcgggggc
      601 cggtttctct tttcttactg acgcttgctg agtccggaga acgaaaaacc gcagtggaca
      661 aattattaat ggagcccctg taccagcagg agatgctgtt atattccaga cacaaaaatg
      721 aactgacgac atggaaaaat aaggaagagc ttttaaaggc
//
LOCUS       DAOTRK010000377          754 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.382, whole genome shotgun sequence.
ACCESSION   DAOTRK010000377 DAOTRK010000000
VERSION     DAOTRK010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.382"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            16..>754
                     /locus_tag="QIE41_004894"
     CDS             16..>754
                     /locus_tag="QIE41_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS3533636.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKGNAGYKESIGGANNFNNKILKHIKSYLIML
                     SIFSIGVYSAHWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMAC"
BASE COUNT          245 a          150 c          149 g          210 t
ORIGIN      
        1 agtgtctatt gacgtatgta ctggattatt aacgataata tcgagttctg gcctgagcac
       61 cgaaaattaa tatcggtaca taacgccgat cttaacgtcg ttctgacaac gccagccagt
      121 cgttgtttat cacttctact tgaagctttt cctgatgtgg ttgcacaaca agattttttc
      181 accagagtct gggaagaaga aggtatgcgt gtgccaacta acacgttata tcagaacata
      241 tccattatca gacgcggatt tcgcgctgtt ggtgatacca cccactcgct tattgcaacc
      301 gtgccgagaa gaggattcaa gattcataat gacattaaca tacaaaatca tgtaataaac
      361 tcttcaacag acgcacatac acacaatgcc ccacctgcca taaaaggtaa tgcggggtac
      421 aaagagagca ttggtggcgc aaacaatttc aataacaaaa tcctcaaaca tataaaaagt
      481 tatctaatta tgttgagcat attttctata ggcgtatatt ctgcacattg gctatggaat
      541 aataatcaac cgaagccatt cttcaaggat tacaagactg ttgcggaaat taatggctgc
      601 cattttaatg tgacagaaga tacaattgat gggttgaaag agttcgataa atataagaca
      661 cggatactgg attcggggat taactgtaaa aaatatccgt ggttatactt ccctcttgca
      721 aaatcctcac cagggatgat tgttatggca tgca
//
LOCUS       DAOTRK010000378          741 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.383, whole genome shotgun sequence.
ACCESSION   DAOTRK010000378 DAOTRK010000000
VERSION     DAOTRK010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.383"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            196..474
                     /locus_tag="QIE41_004895"
     CDS             196..474
                     /locus_tag="QIE41_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDS3533637.1"
                     /translation="MKTITVLLTTVLFSMSASAALTPACEEYYKEIDNFVAKMKEMGT
                     PEAQVNTLKQQYEQSRKQIAALPDASQDMACKQGLDALRQSMSAAGIK"
BASE COUNT          232 a          137 c          153 g          219 t
ORIGIN      
        1 tatttactgg tagtccgtaa tcacaccaca tattctttcg gacttactcc atcatttact
       61 ggatttatat cccagaattc tgataccaag aaaaaacctg tgggaatact cgttttgctg
      121 atattccaat aacattctgc ttttatggcc ttctcaggaa ggttgagtgt ttttgtacaa
      181 aaaaggagtg tatttatgaa aacaattaca gtcttactta ctacagtatt attcagtatg
      241 tcagccagtg cagcactgac tccggcatgt gaagagtatt ataaagaaat cgataatttt
      301 gtagcaaaga tgaaggaaat gggaacacca gaagcacagg ttaatacgct gaaacagcaa
      361 tatgaacaat cccgaaaaca aattgcagct ttacctgacg ccagtcagga tatggcctgt
      421 aagcaaggac tggatgcact gcggcagtca atgagcgctg caggtataaa atgaaggttt
      481 tctgattttt caggggctat tgcaggcccc tttttacatc ataaataatg atatcactta
      541 atgtattaca aaatgttata accagggata ttcccagaat ttataatttc ctcctgtact
      601 tttcaccatg acacagaaac gctatacatc gcggtatttc tgcagggtac gggctggata
      661 tgggtaagcg tctcggtaga gagacggtta atgagtctaa gaattactct tcttttagct
      721 gtgaaataag gaggcgttaa g
//
LOCUS       DAOTRK010000379          735 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.384, whole genome shotgun sequence.
ACCESSION   DAOTRK010000379 DAOTRK010000000
VERSION     DAOTRK010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.384"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            149..370
                     /locus_tag="QIE41_004896"
     CDS             149..370
                     /locus_tag="QIE41_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533638.1"
                     /translation="MQKREPVIIAPDYTNDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPRE"
BASE COUNT          204 a          170 c          202 g          159 t
ORIGIN      
        1 acacaatcat ggaaaaggct tacgaagaat acttcgaagg ccttgccgaa ggcgaagaag
       61 ctctcagctt cagtgagttt aaacaggcgc tttccagctc ggcaaaatct aacggctgat
      121 aagcgaagca gcaccgcgag gaatcagtat gcagaaacga gaacccgtca tcatcgcgcc
      181 agactatacc aatgatgaac tttatgagtg gatgcgccag aaaattaatg cagcgcagga
      241 tttgaaatgg gccaatgaag ccagggctaa gcaggctgaa aatctgtccg ctctggagca
      301 ggatatcacc aatctggaaa aagcagcggc attaagcatt gccagaatga ttacataccc
      361 gcgtgaatag ctaaccaacg aagctaaggt tggtaattaa ggagttctcc acgggtgagg
      421 tggagtgcgt gcgccggaca cgggtgagca tccggcactg acagtttact gaaaggatat
      481 ttccctgaaa agtcagacca taacgcgaaa gcgcacggcg aggtagctgg ttcatagata
      541 gcctgtcgtt aaattttcgt cgaccgtgcg cttccggttg tggcaatccg cgaaatggcg
      601 cggcggtaag tatggcgggg ttattccttc caccgttgag gacaccgggt tgtcaggttg
      661 accatacgct taagtgacaa ccccgctgca acgccctctg ttatcaattt tctggtgacg
      721 tttggcggta tcagt
//
LOCUS       DAOTRK010000380          706 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.385, whole genome shotgun sequence.
ACCESSION   DAOTRK010000380 DAOTRK010000000
VERSION     DAOTRK010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.385"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>706
                     /gene="hcp"
                     /locus_tag="QIE41_004897"
     CDS             <1..>706
                     /gene="hcp"
                     /locus_tag="QIE41_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDS3533639.1"
                     /translation="TGNRWQSGHEDEIFTFSFLNSITNTGLGSQFHGITFCKLIDKST
                     PLFINSINNNEQLFMEFDFYRINRFGRWGKYYYIQLRGAFLSAIHHQIIENQLDTETL
                     TVSYEFILCQHLIANTEFSYLALPENYNCLFLPNSKNQTNNRFKTLNSKAIGRLLAAG
                     GVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEIN
                     SYLGKLRGQQKLLDGID"
BASE COUNT          256 a          112 c          125 g          213 t
ORIGIN      
        1 acaggtaatc gctggcagag cgggcatgag gatgaaatat ttacattctc attcttaaat
       61 agtattacta atacggggct tggttcacag ttccatggta taacattttg taaattaatt
      121 gataaaagca ctccattatt tattaattcc attaacaata atgaacaatt atttatggaa
      181 tttgacttct atcgaataaa tagatttggt agatggggaa agtattatta tatacaacta
      241 agaggcgctt ttttatcggc tattcatcat cagatcattg aaaatcaact ggatacagaa
      301 acattaactg ttagttatga atttatcctc tgtcaacatc ttatcgcaaa taccgagttc
      361 agctatttgg cactccctga aaattataac tgtttgtttt taccaaattc aaaaaaccaa
      421 acaaataatc gtttcaaaac gttaaacagc aaagctattg gcaggctact tgctgctggt
      481 ggcgtataca atgggaacat tgaaggattc agagatactg cggaaaaact gggtggagat
      541 gcaataaaag gctatgatca aatactaaat gaaaaaacag cgggcatagc gatagcaaca
      601 gcatctattc ttttaacaaa gcgttctaat gttgatacat atacagaaat aaatagttac
      661 ttaggcaaac ttagaggtca acaaaaactt cttgatggta tagaca
//
LOCUS       DAOTRK010000381          697 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.386, whole genome shotgun sequence.
ACCESSION   DAOTRK010000381 DAOTRK010000000
VERSION     DAOTRK010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.386"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            412..>697
                     /locus_tag="QIE41_004898"
     CDS             412..>697
                     /locus_tag="QIE41_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDS3533640.1"
                     /translation="MIRIAFLITNKFLEIDSTTLAGYDFSGMNLHRATLNGYCLDGAK
                     FEKTHLRNVMIQHTSTRNAVFHNAALMNAILNNSDFTGSDCSNSRSIGENF"
BASE COUNT          235 a          115 c          150 g          197 t
ORIGIN      
        1 agataaagtc aatgttgaat gttttgaatc aactatagtt gctgttggtc aaattcgaaa
       61 cacaaatgtt ggtgacattg ataatattgg tgaatgtcaa attctcatca ggtataaaga
      121 ccgctggatt gagaataaac tcaaagaaaa aataattgaa taaccatgtc agttatagac
      181 attttagatg gaacgaacca gagaaggtca tgcctccgag ctgggttatg tggacacgga
      241 gctttagcgt agtgaactca acgaagcaag ccggaaacct gccagccctt atcagcacgt
      301 aagggacaac agcgtagtac gcagaataat cctacccacg tagtatggac acaggcctaa
      361 gcgaggttct ggttttcaaa tttttcagga gttagatgat tggaggaaca gatgataaga
      421 atcgcatttt taataaccaa taaatttctt gagatagata gtaccactct tgctggatat
      481 gattttagcg ggatgaatct acatcgtgcc accctcaatg gttattgtct tgatggggcg
      541 aaatttgaaa aaactcattt aagaaatgta atgattcaac atacatcaac tagaaatgct
      601 gtctttcaca atgcggctct gatgaatgct attctgaata actcagattt cactggaagt
      661 gattgtagta attcaaggtc aataggtgaa aatttta
//
LOCUS       DAOTRK010000382          694 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.387, whole genome shotgun sequence.
ACCESSION   DAOTRK010000382 DAOTRK010000000
VERSION     DAOTRK010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.387"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..467
                     /locus_tag="QIE41_004899"
     CDS             <1..467
                     /locus_tag="QIE41_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS3533641.1"
                     /translation="IVTVPIKNNLYNLPFTVLCEVHKNWYKTPNAAPRVFDTGGHQTG
                     AAIILGFGRSTDYDGFPYCDIGGANRRVNENASLEKMVMGMRVKSEQSTCSVSNGHIS
                     SETKTTWSCIQNTAIIRIGGQTTAGLRHLFGHVRNFRIWHKALTDAQVGESI"
     gene            467..>694
                     /locus_tag="QIE41_004900"
     CDS             467..>694
                     /locus_tag="QIE41_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533642.1"
                     /translation="MKDLTLKFADRADFSAFMDSTGYYDDESMQDDILIDVIGNVYKE
                     TGELTEDGEPVCVKEDGYFVNVRIINDSQISS"
BASE COUNT          208 a          129 c          168 g          189 t
ORIGIN      
        1 atatagttac agttccaatt aagaataatc tttataatct tccttttacg gttctttgtg
       61 aggtacataa gaactggtat aaaacgccaa atgcagcgcc acgtgttttt gataccggcg
      121 gtcatcaaac cggagcggct attattcttg gcttcggtcg ttcaacagat tacgacggat
      181 ttccttattg cgatattgga ggagctaaca gacgggtaaa cgaaaacgca tcgcttgaaa
      241 aaatggttat ggggatgcgt gtaaagtcag agcagtctac gtgctcagta agtaacgggc
      301 atatatccag cgaaacaaaa accacatggt cctgtattca gaacaccgca attatccgta
      361 ttggaggcca gactacagcc gggttacgtc atttatttgg tcatgtcagg aatttcagaa
      421 tatggcacaa ggcattgact gatgctcagg tgggggagtc aatctaatga aagatttaac
      481 actcaaattt gccgacaggg ccgacttttc ggcctttatg gatagcactg gctattatga
      541 tgacgagtcg atgcaggatg atattcttat tgacgtgata ggtaacgtgt acaaagaaac
      601 cggagaactg actgaagatg gcgaaccggt atgtgttaag gaagacggat attttgtaaa
      661 cgtgcgcatc attaatgatt cgcaaatatc gtca
//
LOCUS       DAOTRK010000383          681 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.388, whole genome shotgun sequence.
ACCESSION   DAOTRK010000383 DAOTRK010000000
VERSION     DAOTRK010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.388"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            221..415
                     /locus_tag="QIE41_004901"
     CDS             221..415
                     /locus_tag="QIE41_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDS3533643.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          216 a          176 c          142 g          147 t
ORIGIN      
        1 aaacccgcga ggtcgccgcc ccgtaacctg tcggatcgcc ggaaaggacc cgaaaaatga
       61 taataattat catctacata tcacaacgtg catctacgcc atcaaaccac gtcaaataat
      121 caattatgac gcaggtatcg tattaattga tctgcatcaa cttaacgtaa aaacaacttc
      181 agacaataca aatcagcgac actgaatacg gggcaacctc atgtcaacta agaacagaac
      241 ccgcagaaca acaacccgca acatccgctt tcctaaccaa atgattgaac aaattaacat
      301 cgctcttgag caaaaagggt ctgggaattt ctcagcctgg gtcattgaag cctgccgtcg
      361 gagactaacg tcagaaaaga gagcatatac atcaatccaa agtgatgatg gatgaacatc
      421 ccggtttctt ccaccatcgc accggaaaag cgactatgag ggtaaccctg cgtatgtcag
      481 cacagtaaaa cccggtgtgc atcgtttttg attgttcccg cacactcacg cagaaggaat
      541 tccccgtcgg gctacggtca tggttaatgc gggaatacgg cgacgataca gcgcagctaa
      601 aagggtaata gacggataga ccggtttatt tcattccaca ggattctgag tgtccccaac
      661 ttcctccaat agtctgagcg t
//
LOCUS       DAOTRK010000384          667 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.389, whole genome shotgun sequence.
ACCESSION   DAOTRK010000384 DAOTRK010000000
VERSION     DAOTRK010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.389"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..374)
                     /locus_tag="QIE41_004902"
     CDS             complement(<1..374)
                     /locus_tag="QIE41_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3533644.1"
                     /translation="MKRLQAFKFQLRPSGQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQEN"
     gene            382..>667
                     /gene="tnpA"
                     /locus_tag="QIE41_004903"
     CDS             382..>667
                     /gene="tnpA"
                     /locus_tag="QIE41_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDS3533645.1"
                     /translation="MILWSMKKETDIRRGKHCVFLMHVHLVFVTRYRRQIFDHDAKEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVLGR"
BASE COUNT          169 a          156 c          152 g          190 t
ORIGIN      
        1 ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcatcatt ctgtccccgc
       61 tttttgaatc ggggaaaagc agcccgcttc tggaagaagt ttttgtaagc ccgctcaagg
      121 tctttcagtg actgttgcaa tggctgtgac ggggcatctt taagccattg cgtttcagtg
      181 gcatttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg
      241 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
      301 gcgaagcgcc tcatttcaca ctcctgttga ccacttggtc ttaactggaa tttaaatgcc
      361 tgtaatcgtt tcacaggtca aatgatactt tggtctatga aaaaagaaac cgatatccgg
      421 cgtggaaagc attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga
      481 cgccagattt ttgaccatga cgcgaaagaa aaactacgca cttacttttc aaaggtatgt
      541 gctgattttg aagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta
      601 attaactatc ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcgtattg
      661 ggtaggt
//
LOCUS       DAOTRK010000385          666 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.390, whole genome shotgun sequence.
ACCESSION   DAOTRK010000385 DAOTRK010000000
VERSION     DAOTRK010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.390"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..627
                     /locus_tag="QIE41_004904"
     CDS             <1..627
                     /locus_tag="QIE41_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDS3533646.1"
                     /translation="LQPTLYHCDLSVVDEKLNIIQNSFLQHQKMSAYDSMRKNNLFIQ
                     NFVVGCSCAVNASLAEFVLSRIGEQHVKMIAMHDWWLAVTAKLFGRIHFDNTQTILYR
                     QHQGNVLGAKSSGMMRFIRLGLNGQGISRVVSFRKKVCAQNKLLLDVYDKELNLEQKK
                     SIRLVIEGLKENSSIADLLKCFYHGSYMQGFKRNLALIYSVLYTKKRR"
BASE COUNT          217 a           96 c          124 g          229 t
ORIGIN      
        1 ttacaaccta ccttgtatca ttgcgatcta agcgttgttg atgaaaaact taatattata
       61 caaaattcat ttttgcagca tcagaaaatg tcagcgtatg attcaatgag aaaaaataat
      121 cttttcatac aaaattttgt tgttggttgt tcatgtgctg ttaatgcttc acttgcggaa
      181 tttgttcttt cgcgaattgg agagcagcat gtaaaaatga tagctatgca tgactggtgg
      241 ttagccgtga ctgcaaaact ttttggtcga atccattttg ataatactca aacgattctt
      301 tatcgacaac atcagggcaa tgtattaggt gcaaaatcat caggtatgat gcgttttatt
      361 cgattaggat taaatgggca agggatttcg cgagtagtat cttttagaaa aaaagtttgt
      421 gcgcaaaata agcttctttt agatgtctat gataaagagt taaatcttga gcaaaaaaaa
      481 tctatcaggc ttgtaattga gggccttaaa gagaactctt caattgctga ccttttaaaa
      541 tgtttctatc atggtagcta tatgcaaggt tttaaacgta atcttgcctt aatatattca
      601 gttctttaca caaaaaaaag aagatagtgt atccttatga aaaaaattgc tattatcggt
      661 actgtt
//
LOCUS       DAOTRK010000386          662 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.391, whole genome shotgun sequence.
ACCESSION   DAOTRK010000386 DAOTRK010000000
VERSION     DAOTRK010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.391"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(45..371)
                     /locus_tag="QIE41_004905"
     CDS             complement(45..371)
                     /locus_tag="QIE41_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533647.1"
                     /translation="MKYIIFLFRAIWLTLSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(476..>662)
                     /locus_tag="QIE41_004906"
     CDS             complement(476..>662)
                     /locus_tag="QIE41_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS3533648.1"
                     /translation="NEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKVGLRRKMRKA
                     AMDRNYLASVLAGSGLS"
BASE COUNT          226 a          140 c           95 g          201 t
ORIGIN      
        1 tcaatgccca ggttacatat aataataaga gcaattaact taatttattt gtttataatg
       61 cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag ccatattatt
      121 atcgccataa tatacttact atcaatcctt acgccaataa tgtctgaaac agtccctata
      181 aatattagag caataaagaa tactatgcct gttggaaaac agattaccat cattccataa
      241 gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga aagtctatgc
      301 atggaaaaga atagtattaa aagcgataaa gtcagccata tagctctgaa taaaaaaata
      361 atatatttca ttacatcact ccggtgaata caaatctaat aaagcgtcca atgttatttc
      421 cggagcactt catcttacat gtttctaaag aatcattaga ccagtcaggg caagattacg
      481 aaagcccgct ccccgcaagg actgacgcga gatagtttct gtccatggct gcttttcgca
      541 tcttacgtct taaccctacc ttgaatacct tatcattcgt caaaatatta atagcaatgt
      601 gccgtatccc tgaaaataat tctgctgcgt ttcctcttct tattttgcag tcgtcttcat
      661 tc
//
LOCUS       DAOTRK010000387          651 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.392, whole genome shotgun sequence.
ACCESSION   DAOTRK010000387 DAOTRK010000000
VERSION     DAOTRK010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.392"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            308..457
                     /locus_tag="QIE41_004907"
     CDS             308..457
                     /locus_tag="QIE41_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDS3533649.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT          175 a          131 c          179 g          166 t
ORIGIN      
        1 aacagcgatt agatttggca aggtgtatgc agccagcagc ttaacatctc agctaactgg
       61 ctggttacct tcaggttgtc gcaatggcat cggacatccg gtgtcggata actaaacgta
      121 gcgttaaggc gatattgctg agagaccggt taagtggttg cacgggagta atgagtttgg
      181 ggagggtacc agaatgtcag tctggtgcgg tatgctggca gtatcacgtt gattacagca
      241 taaagtaacg agagacgaat ggaagttaaa accagagcag ttccgtcgtt gttcatcccg
      301 ggagacgctg gaaaaagtga tttcccacac gcgttataaa cttacctctg cggaactgga
      361 agccttcaac tctgtggttg atcactggct ggcagaactg acaatgaaca agctttacga
      421 tcgtgttccc gcttcagtct ggaaatatgt catctgagcg ttcggtaatc acaactgcat
      481 cgcaccaggt gttatccgct ttgtgattct tattggctct tctcaggtta ttaaaagctg
      541 aatatgttcc tgttgtcggg aaccaggata ataccgggaa cgttaagcgg tatagcgaca
      601 acaaagaatt cgaaatcaaa agggaatggt gccggaggcg tgggcggtaa t
//
LOCUS       DAOTRK010000388          645 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.393, whole genome shotgun sequence.
ACCESSION   DAOTRK010000388 DAOTRK010000000
VERSION     DAOTRK010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.393"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..625
                     /locus_tag="QIE41_004908"
     CDS             <1..625
                     /locus_tag="QIE41_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS3533650.1"
                     /translation="NLSEFFNIEHDKFIYCDNVQNPDEVIKKSDDIDTSGFIFENDKI
                     YFITLGRLSVEKDQQKLINAFCRLQKLYPNIELLILGDGPLKIDLQRQIITLGLEKSV
                     HLLGRISNPFPLLKRADCFVLSSNHEGQPMVLFEAMILDKPIISTDITGSRSALEGRS
                     GVLVENSVDGLFNGMRDFILGRLEFKHFDIESYQKNALSMFYEKCLH"
BASE COUNT          210 a           97 c          116 g          222 t
ORIGIN      
        1 taacttaagc gaatttttta atattgagca tgataagttt atttattgtg ataatgttca
       61 gaatccagat gaggtgatta aaaaatctga tgatattgat acaagtggat ttatttttga
      121 aaatgataaa atctacttta taactttggg cagactttca gtcgaaaaag atcaacaaaa
      181 gttgattaat gccttttgca gattacaaaa attatatcct aatattgagt tattaattct
      241 tggagacggc cccctcaaga ttgatttaca acgccagatt ataactttag gacttgaaaa
      301 atccgtacat cttctgggac gcatatcaaa tccttttcca ctgctaaaga gagctgactg
      361 ttttgtcctc tcttcaaatc atgaagggca acctatggtt cttttcgaag caatgattct
      421 agataaacct ataatatcaa ctgacattac tggttctaga agtgctcttg aagggcgttc
      481 tggtgtatta gttgaaaata gtgtagacgg tctcttcaac ggaatgcggg actttatctt
      541 gggaaggttg gagtttaaac attttgacat tgaatcttat caaaaaaatg ctttgtcgat
      601 gttttatgaa aaatgccttc attaaagcat taatctatat catga
//
LOCUS       DAOTRK010000389          629 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.394, whole genome shotgun sequence.
ACCESSION   DAOTRK010000389 DAOTRK010000000
VERSION     DAOTRK010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.394"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..575
                     /locus_tag="QIE41_004909"
     CDS             <1..575
                     /locus_tag="QIE41_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDS3533651.1"
                     /translation="SAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPGSDDLNGINV
                     KYRYEFTDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAGPSVRVNEWF
                     SAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPT
                     ESVTIDLAYEGSGSGDWRTDAFIVGIGYRF"
BASE COUNT          136 a          151 c          190 g          152 t
ORIGIN      
        1 tgtccgcagt agtctggcag gtcgccgctg ctacgccagc gagtgcagca gaacatcagt
       61 ccacgctgag cgcggggtat ctccatgcct cgacgaacgt tcccggtagt gatgatctga
      121 acgggattaa cgtgaaatac cgttatgagt ttacggacgc gctggggctg attacgtcct
      181 tcagttatgc caatgctgag gatgagcaaa aaacgcgcta cagcgatacc cgctggcatg
      241 aagattccgt gcgtaaccgc tggttcagcg tgatggcggg gccgtctgta cgcgtgaatg
      301 aatggttcag cgcgtatgcg atggtgggtg tggcttacag ccgtgtgtcg actttctccg
      361 gggattatct ccgcgtaact gacaacaagg ggaaaacgca cgatgtgctg accggaagtg
      421 atgacggtcg ccacagcaac acgtctctgg cgtggggggc tggcgtgcag tttaatccga
      481 ccgaatccgt gaccattgac cttgcttatg aaggttccgg tagtggcgac tggcgaacgg
      541 atgcatttat tgttggtatc ggataccgtt tctgacaaca gacgccgatt tatcttctgt
      601 aaatattgtt atgatacgca ggttcatcc
//
LOCUS       DAOTRK010000390          627 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.395, whole genome shotgun sequence.
ACCESSION   DAOTRK010000390 DAOTRK010000000
VERSION     DAOTRK010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.395"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>627
                     /locus_tag="QIE41_004910"
     CDS             <1..>627
                     /locus_tag="QIE41_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDS3533652.1"
                     /translation="GTGLATTGKGIQVVEAINGATTEEGAFVQGNMLQAGAFNYTLNR
                     DSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQG
                     GHLGHDNNGGIARGATPESSGSYGLVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVK
                     DDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASSDN"
BASE COUNT          135 a          166 c          201 g          125 t
ORIGIN      
        1 ggtacggggc tggcgaccac cggtaagggt attcaggtgg ttgaggccat taacggtgcc
       61 accacggagg aaggggcttt tgtccagggg aatatgctgc aggccggggc ctttaactac
      121 accctcaacc gggacagtga tgagagctgg tatctgcgca gtgaagaacg ttatcgtgct
      181 gaagtccccc tgtacgcctc catgctgaca caggcaatgg actatgaccg gattctggca
      241 ggctcccgca gccatcagac cggtgtaaat ggtgaaaaca acagcgtccg tctcagcatt
      301 cagggcggtc atctcggtca cgataacaat ggcggtattg cccgtggggc cacgccggaa
      361 agcagcggca gctatggcct cgtccgtctg gagggtgacc tactcagaac agaggttgcc
      421 ggtatgtcac tgacgacagg agtgtatggt gctgcaggcc attcttccgt tgatgttaag
      481 gatgatgacg gttcccgcgc cggcacggtc cgggatgatg ccggcagtct gggcggatac
      541 ctgaatctgg tacacacgtc ctccggcctg tgggctgaca ttgtggcaca gggaacccgc
      601 cacagcatga aagcgtcatc ggacaat
//
LOCUS       DAOTRK010000391          619 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.396, whole genome shotgun sequence.
ACCESSION   DAOTRK010000391 DAOTRK010000000
VERSION     DAOTRK010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.396"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..482
                     /locus_tag="QIE41_004911"
     CDS             <1..482
                     /locus_tag="QIE41_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533653.1"
                     /translation="FTIFTKGTIFSAFPVLNNKTMWRWYQNEDIGEYYWQTELGICKN
                     NKFTPSGARLLIRVGSLRLNENHATKGTLQELLNTAEKTAFLGDRFRSYIRAGIYQKK
                     SSDPAQLLAVLDNSIMVKYFKDEKPTYARMTAHLPNKDESYECLTKVQHELLRSEEK"
BASE COUNT          219 a          104 c          125 g          171 t
ORIGIN      
        1 gctttaccat tttcactaag ggtactattt tttccgcgtt tcctgtctta aataataaaa
       61 ccatgtggag atggtatcag aatgaggaca taggtgagta ttattggcaa acagaacttg
      121 gtatatgtaa aaataacaaa ttcacaccaa gcggggcgag actcttaatt cgcgtcggat
      181 cactacgcct taatgaaaat catgcaacca aaggaacgtt gcaggagttg ctaaatacag
      241 cagaaaaaac ggcgtttctc ggcgatcggt ttagaagtta tataagagcg ggtatctatc
      301 aaaaaaagag cagtgatcca gctcagttgt tggcagtcct tgataattca atcatggtta
      361 aatattttaa agatgagaag ccaacttatg ctcgaatgac agcacatctc ccgaacaaag
      421 atgagtctta cgaatgttta acaaaagtac agcatgaact gcttcgtagt gaagaaaaat
      481 aaaatctctt cctgagaaaa taaattaact attcgaaaaa atatgttctt aatgggtatc
      541 caggaacacc agaaggtcga tttggaaaaa tggattccga tggtaagaat atcatatatt
      601 ctgaatgtaa tgttttacc
//
LOCUS       DAOTRK010000392          611 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.397, whole genome shotgun sequence.
ACCESSION   DAOTRK010000392 DAOTRK010000000
VERSION     DAOTRK010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.397"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            13..>611
                     /locus_tag="QIE41_004912"
     CDS             13..>611
                     /locus_tag="QIE41_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5B family transposase"
                     /protein_id="HDS3533654.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSG"
BASE COUNT          156 a          168 c          158 g          129 t
ORIGIN      
        1 tgatatgaga tcatgtttgt catctggagc catggaacag ggttcatcat gagtcatcaa
       61 cttaccttcg ccgacagtga attcagcagt aagcgccgtc agaccagaaa agagattttc
      121 ttgtcccgca tggagcagat tctgccatgg caaaacatgg tggaagtcat cgagccgttt
      181 taccccaagg ctggtaatgg ccggcgacct tatccgctgg aaaccatgct acgcattcac
      241 tgcatgcagc attggtacaa cctgagcgat ggcgcgatgg aagatgctct gtacgaaatc
      301 gcctccatgc gtctgtttgc ccggttatcc ctggatagcg ccttgccgga ccgcaccacc
      361 atcatgaatt tccgccacct gctggagcag catcaactgg cccgccaatt gttcaagacc
      421 atcaatcgct ggctggccga agcaggcgtc atgatgactc aaggcacctt ggtcgatgcc
      481 accatcattg aggcacccag ctcgaccaag aacaaagagc agcaacgcga tccggagatg
      541 catcagacca agaaaggcaa tcagtggcac tttggcatga aggcccacat tggtgtcgat
      601 gccaagagtg g
//
LOCUS       DAOTRK010000393          573 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.398, whole genome shotgun sequence.
ACCESSION   DAOTRK010000393 DAOTRK010000000
VERSION     DAOTRK010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.398"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..543)
                     /locus_tag="QIE41_004913"
     CDS             complement(<1..543)
                     /locus_tag="QIE41_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDS3533655.1"
                     /translation="MQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNN
                     GGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCR
                     KWVKPNLTCTPQDDPQYPGMETKTASDYLPQTTWPTTQLPPGYTCAEPYLFPDINKPD
                     GPATAGIDDLGEILAKMKQRT"
BASE COUNT          132 a          125 c          131 g          185 t
ORIGIN      
        1 tgttctctgt ttcattttag ctaaaatttc acccaaatca tctatccctg ctgttgctgg
       61 cccatcgggt ttattaatgt ctgggaataa atagggttct gcacaagtat atcctggtgg
      121 taattgagtt gttggccaag ttgtctgtgg caggtaatca cttgctgtct ttgtctccat
      181 ccctggatac tggggatcat cctgcggcgt acaagtcaga ttgggtttta cccacttcct
      241 gcaatacagt gtgtaatcgt aaggacttaa tgctctgaag aatgttttga aaacattagg
      301 cgcactactg gcctggtcaa taggatatgg cagtcttaat tgtttcgcta tgtctggcaa
      361 cttacttgga tcgggcgtag gcggtttgtt gggtctcgct ccatatccgc cattatttaa
      421 gtgtccatat ttagatgcca gtcccattga atctatatac tgaaccggat ttaacggata
      481 ctgataaaaa ttccatcccc ccttcagtcc aatcggatcc tgagtgatat accgcccctg
      541 cagcgggtca taatagcggt ggcggttgta ata
//
LOCUS       DAOTRK010000394          565 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.399, whole genome shotgun sequence.
ACCESSION   DAOTRK010000394 DAOTRK010000000
VERSION     DAOTRK010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.399"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            119..>565
                     /locus_tag="QIE41_004914"
     CDS             119..>565
                     /locus_tag="QIE41_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDS3533656.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT          130 a          124 c          140 g          171 t
ORIGIN      
        1 tcactaattg attagtcata gccagcgata tacgctatgc gaaaatgcag atgacaatga
       61 gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga gcattagcat
      121 gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga tggttgttta
      181 ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct tcgaacaatc
      241 tttttattcc agattggtag cgattccggt gaacatctta attgcatggc catacggtat
      301 gtaccgcgat ctgtttatgc gcgcagcacg caaagttagc ccgtcgggct ggataaaaaa
      361 tctggcggat atcatggctt atgtgacgtt ccagtcaccg gtgtatgtgg cgatcttgtt
      421 agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca tcgttgtttc
      481 gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac tgtttaaagt
      541 cagccgttac cagcaggtaa aagcc
//
LOCUS       DAOTRK010000395          554 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.400, whole genome shotgun sequence.
ACCESSION   DAOTRK010000395 DAOTRK010000000
VERSION     DAOTRK010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.400"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..546
                     /locus_tag="QIE41_004915"
     CDS             <1..546
                     /locus_tag="QIE41_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533657.1"
                     /translation="IKDCTALNCVSGPAFLKDVTVENFRTGDIFLIYSTMFHHVTLKG
                     KLGAIKINKKDYVRDYDSHQRHIEMMRTRFYSQIDWAMDISQAKFLSFSCKGIPAKLI
                     RRDSETQFVVTRKRFNSMDVLNAEFKGQYPFVYLLLELFLESDEDDQVLVTPLGKAKK
                     YYQPILEGFRELRRAGILEPN"
BASE COUNT          194 a           79 c          112 g          169 t
ORIGIN      
        1 attaaagatt gcactgctct taattgtgta agtggcccag cttttttaaa agatgtaact
       61 gttgagaatt ttaggaccgg tgatattttt cttatttatt ccactatgtt tcatcatgta
      121 acattaaaag gaaagcttgg ggcaataaaa ataaataaaa aagattatgt gcgtgactat
      181 gatagccatc aaagacatat tgagatgatg agaacgagat tttattctca gatagattgg
      241 gcgatggata tatcacaggc gaagttttta agtttctcct gcaaaggtat cccagcaaaa
      301 ttaattagac gagatagtga gactcaattt gttgtaacga ggaaacgttt caactctatg
      361 gatgtactaa atgcagaatt taaaggacaa tacccgtttg tttatttact gctggagctc
      421 tttttagaat cagatgaaga tgaccaggta ttagttacac cattaggcaa agcaaaaaaa
      481 tattatcagc caattctcga aggatttaga gagttaagac gagcaggaat actcgaacct
      541 aactaaataa gggt
//
LOCUS       DAOTRK010000396          537 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.401, whole genome shotgun sequence.
ACCESSION   DAOTRK010000396 DAOTRK010000000
VERSION     DAOTRK010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.401"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>537
                     /locus_tag="QIE41_004916"
     rRNA            <1..>537
                     /locus_tag="QIE41_004916"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          136 a          119 c          179 g          103 t
ORIGIN      
        1 ggggaggaag ggagtaaagt taataccttt gctcattgac gttacccgca gaagaagcac
       61 cggctaactc cgtgccagca gccgcggtaa tacggagggt gcaagcgtta atcggaatta
      121 ctgggcgtaa agcgcacgca ggcggtttgt taagtcagat gtgaaatccc cgggctcaac
      181 ctgggaactg catctgatac tggcaagctt gagtctcgta gaggggggta gaattccagg
      241 tgtagcggtg aaatgcgtag agatctggag gaataccggt ggcgaaggcg gccccctgga
      301 cgaagactga cgctcaggtg cgaaagcgtg gggagcaaac aggattagat accctggtag
      361 tccacgccgt aaacgatgtc gacttggagg ttgtgccctt gaggcgtggc ttccggagct
      421 aacgcgttaa gtcgaccgcc tggggagtac ggccgcaagg ttaaaactca aatgaattga
      481 cgggggcccg cacaagcggt ggagcatgtg gtttaattcg atgcaacgcg aagaacc
//
LOCUS       DAOTRK010000397          534 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.402, whole genome shotgun sequence.
ACCESSION   DAOTRK010000397 DAOTRK010000000
VERSION     DAOTRK010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.402"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>534)
                     /locus_tag="QIE41_004917"
     CDS             complement(<1..>534)
                     /locus_tag="QIE41_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533658.1"
                     /translation="VNNLEALAPGIEKLKQTSIEMVTLLNALQPGGKCIITGDFQKEL
                     AYLQNVILYNDSSLRMDFFGYNARIIQRSDNTCELTINEPLKNQEISTGNINVNCPLK
                     DIYNEIRRLNVIFSCGTGGIVDLSSLDLRNVDLELYDFTDKHMANTILNPFKLDDTDF
                     TNANMFQVNFVSSTQNAT"
BASE COUNT          164 a           89 c           96 g          185 t
ORIGIN      
        1 gtggcgtttt gtgttgaact aacgaaattt acctgaaaca tgttggcatt agtaaaatct
       61 gtgtcatcca atttaaaagg atttaaaatt gtattggcca tatgtttatc tgtaaaatca
      121 taaagttcta aatcgacatt ccgtaagtcc agagaggata gatcaactat acctccagtc
      181 ccacaactaa agattacgtt caacctcctg atttcattgt aaatgtcttt caatgggcaa
      241 ttaacattga tattgcctgt ggatatttcc tggtttttta acggttcatt aatggtaagt
      301 tcacaggtat tgtctgatcg ttgaataatt cgggcgttat aaccaaaaaa atccatacga
      361 agagacgaat cattataaag aattacattt tgtaagtacg ctaattcttt ttgaaaatca
      421 ccagtgataa tgcatttccc acctggttgt agcgcgttaa gtaaagtgac catttcaata
      481 gaggtctgtt ttagtttttc tatacctggt gctagtgctt cgaggttatt aact
//
LOCUS       DAOTRK010000398          533 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.403, whole genome shotgun sequence.
ACCESSION   DAOTRK010000398 DAOTRK010000000
VERSION     DAOTRK010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.403"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            64..279
                     /gene="bdm"
                     /locus_tag="QIE41_004918"
     CDS             64..279
                     /gene="bdm"
                     /locus_tag="QIE41_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDS3533659.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            381..518
                     /gene="sra"
                     /locus_tag="QIE41_004919"
     CDS             381..518
                     /gene="sra"
                     /locus_tag="QIE41_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDS3533660.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
BASE COUNT          166 a          121 c          118 g          128 t
ORIGIN      
        1 atgtgcttta taaatctgcg atccgtagca gacaccaaaa atacacagac acggagaatc
       61 gctatgttta cttattatca ggcagaaaat tcaacagcag aacccgctct ggttaatgcc
      121 attgagcaag gccttcgggc agagcacggt gttgtcactg aagatgacat tctcatggag
      181 ctgaccaaat gggtggaagc ttctgataac gacatcctca gtgatatcta ccagcaaacg
      241 atcaattatg tggtcagtgg ccagcaccct acgctttaag gtgctatgct tgatcggcaa
      301 cctaatttag gggtttagca cgtgtttctt cgctacggcg atgttgtcct taaaactagc
      361 aacaggattg aggagttaaa atgaaatcga accgtcaggc acgtcatatt cttggactgg
      421 accataaaat ttctaaccag cgcaaaatag ttaccgaagg tgacaaatcc agcgtggtaa
      481 ataacccaac cggcagaaaa cgccccgctg aaaagtaatt cataaccatc agt
//
LOCUS       DAOTRK010000399          533 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.404, whole genome shotgun sequence.
ACCESSION   DAOTRK010000399 DAOTRK010000000
VERSION     DAOTRK010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.404"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(175..345)
                     /locus_tag="QIE41_004920"
     CDS             complement(175..345)
                     /locus_tag="QIE41_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533661.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(460..>533)
                     /locus_tag="QIE41_004921"
     CDS             complement(460..>533)
                     /locus_tag="QIE41_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDS3533662.1"
                     /translation="VLLNRVDTSTAPDIEWPANPVRE"
BASE COUNT          139 a          140 c          109 g          145 t
ORIGIN      
        1 ttttacttga ttgtaacttt ttcgtttttt tggggctaat tgacgcgcaa tgctgggcgg
       61 ggagcgcaca gccaaaaaaa ccgaaattga ccgctaatta ctgccaatca tcatcgctct
      121 gattgagcgt aaatcattca ggttattcag ttcttccttc tgctctcgct gacgtcaata
      181 aatcccatca ttgcgatcaa cctcatcctt aaccattgat gcgagcagtt cttccagttt
      241 ctgcgctgat agctttacct ggtgatcttc agcatcttcc cgggctattg tggttcgcgc
      301 agtggctgat tttgctgaca tcaccacagg gggcagacgg accattgaac catggcctcc
      361 ggaacaaata agggcaaaaa taacaaccac tatcgaaagc tcacaaacta accgcagcac
      421 gttcctgcat acgacgtgtc tgcggcataa tcccaatgat tactccctga caggatttgc
      481 aggccactca atatcaggtg cagttgatgt atcaacacga ttcaacaata ccc
//
LOCUS       DAOTRK010000400          511 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.405, whole genome shotgun sequence.
ACCESSION   DAOTRK010000400 DAOTRK010000000
VERSION     DAOTRK010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.405"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>511)
                     /locus_tag="QIE41_004922"
     CDS             complement(<1..>511)
                     /locus_tag="QIE41_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HDS3533663.1"
                     /translation="LLGLKKILDANKIQFFLVSGTLLGCIRDKKLLKHDKDIDIGVWS
                     ETNLSVLSTKIACSGLFDIAPMRSPYTLRIKHVNGVAIDIFFHYRDHDSYWHAGSKLR
                     WNNTPFNLISYGFLGNVFLIPENYDLYLTENYGNWMQEKMKFDSAFDTPNHEIVNMYE
                     LKIHAYKKLI"
BASE COUNT          169 a           92 c           68 g          182 t
ORIGIN      
        1 tattagcttt ttatacgcgt gtatttttaa ttcatacata ttaactattt catgattggg
       61 tgtatcgaat gcagagtcaa atttcatttt ttcttgcatc cagtttccat aattctcagt
      121 caaatataaa tcataatttt ctggtattaa aaacacgttc ccaagaaaac cataagaaat
      181 caaattaaat ggagtattgt tccatctcag ctttgaaccc gcatgccaat agctgtcatg
      241 atctcgataa tgaaaaaata tgtcgattgc cacaccatta acatgtttta ttctcaacgt
      301 atacggtgag cgcataggtg caatatcaaa caagccagag caagcaattt tcgttgataa
      361 tacggataga tttgtttcgc tccatactcc tatatcaata tctttgtcat gttttagtaa
      421 ttttttatca cgaatacaac caagtaatgt tccgctaact aaaaaaaact gtattttatt
      481 agcatccaat atttttttta gacctaataa t
//
LOCUS       DAOTRK010000401          508 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.406, whole genome shotgun sequence.
ACCESSION   DAOTRK010000401 DAOTRK010000000
VERSION     DAOTRK010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.406"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>508
                     /locus_tag="QIE41_004923"
     CDS             <1..>508
                     /locus_tag="QIE41_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDS3533664.1"
                     /translation="LAKYILQNVYIVFVQTDDFASSFRLFNVLNSRGLPLSNADLLKN
                     ALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLSEKKDRDRVLQKGFEAF
                     IENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHPSIRRKIASLSNLGVDEWIPPV
                     MAFMNRMAR"
BASE COUNT          169 a           74 c           99 g          166 t
ORIGIN      
        1 gcttgctaaa tatattttgc aaaatgtata tatcgtattt gtgcaaactg atgatttcgc
       61 atcatcattt agactcttta atgtattaaa tagtcgaggt ttacctttaa gtaatgcaga
      121 tcttttaaaa aatgcactgt ttgaatcagc ttctacgcat aataaaaaat cagagcaaat
      181 agaatcagca tggtcgcaaa ttgaagatat ggttggagtg agacggcttg ataaatttct
      241 tactcttcat aagctttccg agaaaaaaga tcgtgacagg gtattacaaa aaggttttga
      301 agcttttatt gaaaatctcc agcagcaatt tgatggagat gctattgcta tgtcactgat
      361 gttggttaat tctgcaaaaa attatactaa gattttagag aatgattttg aacacccttc
      421 aatacgacga aaaatagcat cgctaagtaa tttaggggta gatgagtgga ttcctccggt
      481 tatggcattc atgaatcgaa tggctcgt
//
LOCUS       DAOTRK010000402          508 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.407, whole genome shotgun sequence.
ACCESSION   DAOTRK010000402 DAOTRK010000000
VERSION     DAOTRK010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.407"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(336..410)
                     /locus_tag="QIE41_004924"
     tRNA            complement(336..410)
                     /locus_tag="QIE41_004924"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Gly,seq:tcc)
     gene            complement(414..489)
                     /locus_tag="QIE41_004925"
     tRNA            complement(414..489)
                     /locus_tag="QIE41_004925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Thr,seq:tgt)
BASE COUNT          152 a          137 c          101 g          118 t
ORIGIN      
        1 tttattgtaa taagaatcga ggtgtcgggt gcctcccgaa atatccatct ctacaacaga
       61 tattgtgatt ccccgccaaa ccactatcta aaccaccctc gcactgagga acacctctgc
      121 ggtgctttta caacaccaga atgatgcatc accgaccctg ccaggaaata caaaatctcc
      181 accgataatg caccattctg ctgccgtaaa aaaattagca ctgtgactac aaccgacctc
      241 caatcatagt cagagaacgg attgtctttt taaaacaacc caccccacgt aataaaaaat
      301 acgccagtgc cacgatacaa taaggcttgt ttctctggag cgggtagcgg gaatcgaacc
      361 cgcatcatca gcttggaagg ctgagataat agccattata cgatacccgc atatggtgcc
      421 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
      481 taagtcggca ttggttcttc aggggagc
//
LOCUS       DAOTRK010000403          506 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.408, whole genome shotgun sequence.
ACCESSION   DAOTRK010000403 DAOTRK010000000
VERSION     DAOTRK010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.408"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            1..506
                     /locus_tag="QIE41_004926"
                     /pseudo
     CDS             1..506
                     /locus_tag="QIE41_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          172 a           88 c          115 g          131 t
ORIGIN      
        1 gggagcgatt catgtcatta gtgcgttctc aacaatgcac attctggtcc tggggcagat
       61 caggacggat gagaaatcta atgagatcac agctatccct gaacttctta acattttgta
      121 tattaaagag aaaatcatca caactgatgc gatgggttgc cagaaagata ttgcagagaa
      181 gatacaaaaa cagggtggtg attacttatt cgctgtaaaa ggaaaccagg ggcggctgaa
      241 taaagccttt gaggaaacat ttccgctgaa agaattaatt aatccagagc atgacagtta
      301 cgcaattagt gaaaagagtc acggcagaga agaaatccgt cttcatattg tttgcgatgt
      361 ccctgatgaa cttattgatt ttacatttga atggaaagga ctgaaaaatt atacgtggca
      421 gtctcctttc ggtcaataat agcagaacaa aagaaagagc cagaaatgac ggtcagatat
      481 tatatcagct ctgctgattt aaccgc
//
LOCUS       DAOTRK010000404          502 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.409, whole genome shotgun sequence.
ACCESSION   DAOTRK010000404 DAOTRK010000000
VERSION     DAOTRK010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.409"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..375
                     /locus_tag="QIE41_004927"
     CDS             <1..375
                     /locus_tag="QIE41_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDS3533665.1"
                     /translation="LNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPG
                     KVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLA
                     MLFTLANLFRADQMIRQWERSH"
BASE COUNT          155 a          119 c          139 g           89 t
ORIGIN      
        1 ctcaatcagc tgggtaatct gctgcatgga gaggagcaat ttgtctcagc cgatgccggc
       61 taccaagggg cgccacagcg cgaggagctg gccgaggtgg atgtggactg gctgatcgcc
      121 gagcgccccg gcaaggtaag aaccttgaaa cagcatccac gcaagaacaa aacggccatc
      181 aacatcgaat acatgaaagc cagcatccgg gccaaggtgg agcacccatt tcgcatcatc
      241 aagcgacagt tcggcttcgt gaaagccaga tacaaggggt tgctgaaaaa cgataaccaa
      301 ctggcgatgt tattcacgct ggccaacctg tttcgggcgg accaaatgat acgtcagtgg
      361 gagagatctc actaaaaact ggggataacg ccttaaatgg cgaagaaacg gtctaaatag
      421 gctgattcaa ggcatttacg ggagaaaaaa tcggctcaaa catgaagaaa tgaaatgact
      481 gagtcagccg agaagaattt cc
//
LOCUS       DAOTRK010000405          497 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.411, whole genome shotgun sequence.
ACCESSION   DAOTRK010000405 DAOTRK010000000
VERSION     DAOTRK010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.411"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            31..>497
                     /locus_tag="QIE41_004928"
     CDS             31..>497
                     /locus_tag="QIE41_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533666.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVS"
BASE COUNT          162 a           92 c          110 g          133 t
ORIGIN      
        1 tgagataaac tcaacgtcaa gaggtgggtt atgattagat atagtggaat atttctatcg
       61 ctgttaatgc tttcaggttg tgcaactgaa gcattgaaaa acgaacaagc tgaaactgtt
      121 attactgcgg gtaacaaagc agtagcggcg aatgcaagtt tctttaaaca atactatgct
      181 tctcgtgaaa acttccttgt taatttctac gcgactaatc cggattgtgc tctgcgacct
      241 gattatacca ttgatatagg cggtgatggg aatggcggaa aattatgcca gcgttcaggg
      301 ccaaatacaa aacaagtaca attagtccct ttcgagaaag aaaaattact ggcttcaatg
      361 gaagtcatta atagcatgag cgcttatgtg gaacttctga caaaggatat ggcaactacg
      421 gaaagtcagc aagtggcatt acttaaacag gctctggatc acgcaaacaa cgcacaaaca
      481 ctacttggtg tttcaca
//
LOCUS       DAOTRK010000406          472 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.414, whole genome shotgun sequence.
ACCESSION   DAOTRK010000406 DAOTRK010000000
VERSION     DAOTRK010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.414"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>472
                     /locus_tag="QIE41_004929"
     rRNA            <1..>472
                     /locus_tag="QIE41_004929"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          118 a          113 c          142 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DAOTRK010000407          471 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.415, whole genome shotgun sequence.
ACCESSION   DAOTRK010000407 DAOTRK010000000
VERSION     DAOTRK010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.415"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     repeat_region   37..309
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=37..65
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          128 a          124 c          106 g          113 t
ORIGIN      
        1 accgcatggg agtccagatt acaaattcca gaatgggagt tccccgcgcc agcggggata
       61 aaccgcgcca gtctgaggtt ttccggtcat acatcgtgag ttccccgcgc cagcggggat
      121 aaaccgaccg ttgctacaca tatgatcagg catgtgccga gttccccgcg ccagcgggga
      181 taaaccggac gatgagacgc cctggtgtgc cgcgttcgtg agttccccgc gccagcgggg
      241 ataaaccgcg tttagctccg caatgttgga tatcactgat gagttccccg caccagcggg
      301 aataaaccac catataaccc gttatctctt tctcaagttt ttatattagc agtactcgta
      361 ttaagcaaca tatccacgta acacctcatg ttcaaaatag ctctccatat atgagaagtt
      421 cacaattatc gatacaaaaa atcaaattta atcaaagtgt tatttgtata a
//
LOCUS       DAOTRK010000408          467 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.416, whole genome shotgun sequence.
ACCESSION   DAOTRK010000408 DAOTRK010000000
VERSION     DAOTRK010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.416"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..232
                     /locus_tag="QIE41_004930"
     CDS             <1..232
                     /locus_tag="QIE41_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533667.1"
                     /translation="QTLVTRKMETEAFGDSNATRTTDAFLETECVENVATTEIIKATE
                     ESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            333..461
                     /locus_tag="QIE41_004931"
                     /pseudo
     CDS             333..461
                     /locus_tag="QIE41_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
BASE COUNT          125 a          117 c          117 g          108 t
ORIGIN      
        1 tcagacgctg gtgaccagaa aaatggaaac ggaagccttt ggtgacagta atgccacgcg
       61 aaccacagac gcatttctgg aaacggagtg cgtggaaaat gtcgccacaa ccgaaatcat
      121 taaagcaact gaagaaagta acggccatcg tgtcagtctg ccgttatcgg ttttcgatcc
      181 ccaggactac catccactgc tgattaccgt ttccggaaaa aatgttaact gatccctgaa
      241 gtcagtttca tgcttcagcc tctgccacaa cgtatggcag aggctattta tcaggagaaa
      301 ccagatatgc cgggttacac acagagtatg tcctggctga aggatctttc tcatacggtc
      361 aggcggttgc tgtcataacc gcttaccgca atgtgtttat ccaggatgac ccgggtacgc
      421 attttcgtct ggtgatccgc aacgcggaag gtcagcgacg ctgacgc
//
LOCUS       DAOTRK010000409          457 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.418, whole genome shotgun sequence.
ACCESSION   DAOTRK010000409 DAOTRK010000000
VERSION     DAOTRK010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.418"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..316
                     /gene="iraM"
                     /locus_tag="QIE41_004932"
     CDS             <1..316
                     /gene="iraM"
                     /locus_tag="QIE41_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDS3533668.1"
                     /translation="TVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFTLTHTGIM
                     LNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQQ
                     SP"
BASE COUNT          150 a          104 c           82 g          121 t
ORIGIN      
        1 tacagttatc cagccatcat gcggaatatc tttttcagtc atatggagta aaataaaatt
       61 aataatctgg tatcaatcgg atgctttctt acctcctgaa agtatattta cactgactca
      121 cacaggcatc atgctcaata acaaagtgct gcctgtaacc atttacaacg tagtaccatt
      181 caataaaaca ttctggaatt taatcaaaaa cagccaggaa tgccctacaa atacagataa
      241 cgtattgaat gaatgcttta ataaccgttg cactctgcaa atatgtcctt atgggctaaa
      301 acaacaaagt ccataaggag tttactcaca tctgacaaaa tcaatataaa cagcccctcc
      361 ggagaggggc tggagagtgg cgctatgtgc cattgcatgg tgccgggtgc ctcccggtga
      421 attcagtacc agcacctgaa tccgcgatta tcccata
//
LOCUS       DAOTRK010000410          453 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.420, whole genome shotgun sequence.
ACCESSION   DAOTRK010000410 DAOTRK010000000
VERSION     DAOTRK010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.420"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>453)
                     /locus_tag="QIE41_004933"
     CDS             complement(<1..>453)
                     /locus_tag="QIE41_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDS3533669.1"
                     /translation="YINDVRILDWCFNKSMQYIGDSAKIKHACSVINYIDFSRDQSKD
                     FSCDQSKIKIAETLFFNLDKEPYKNSCELHELIWDKLVAYVNDFNLSNQEKSRLILRL
                     FDDVKLLFNEVPVSILVNDIFLKGFFMKQPDFAKWYFYQLLKKYEGEQ"
BASE COUNT          157 a           84 c           56 g          156 t
ORIGIN      
        1 gctgttcgcc ctcatacttc ttaagtaatt gatagaagta ccacttcgca aaatcaggtt
       61 gcttcatgaa aaaacccttc aaaaaaatat catttactaa aatgctgaca ggcacttcat
      121 taaatagcaa cttaacatca tcaaataacc ttagtattaa gcgtgatttt tcttgattgc
      181 ttaagttaaa atcattgaca tatgcaacca acttatccca aatcaattca tgtaattcac
      241 agctattttt atagggttct ttatcgagat tgaaaaataa ggtctcagct attttaattt
      301 tagattgatc gcagctaaaa tctttagatt gatcgcggct aaaatcaata taatttatca
      361 cagagcatgc atgctttatc tttgctgaat caccaatata ttgcatgctt ttattaaaac
      421 accaatccag tattcttaca tcattaatat atg
//
LOCUS       DAOTRK010000411          439 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.421, whole genome shotgun sequence.
ACCESSION   DAOTRK010000411 DAOTRK010000000
VERSION     DAOTRK010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.421"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>439)
                     /locus_tag="QIE41_004934"
     CDS             complement(<1..>439)
                     /locus_tag="QIE41_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDS3533670.1"
                     /translation="AGQTVSVMADNSATVSPTVTTEPDGTVEISITSQTAGTSTGTAS
                     INNSSLSRNVTFIADVRTAKIADLVVIKDDSVADGVMANMLRARVTDAFGNVLAGQTV
                     SVTADNGATVAPVVITGPDGTVEISVTSQTAGTSAITASINNSS"
BASE COUNT           66 a          145 c          122 g          106 t
ORIGIN      
        1 ggctgctgtt gttgatactg gcggtgatcg cactggttcc ggcggtctgg ctggtgactg
       61 agatctccac cgtgccgtcc ggccctgtga tgaccaccgg agcaaccgtt gcgccgttgt
      121 ccgccgtcac gctaaccgtc tggccggcaa gtacattccc gaacgcatcg gtgaccctcg
      181 cccgcagcat attcgccatt acaccatctg ccactgagtc gtccttaatg accaccagat
      241 cagcgatctt cgccgtcctg acatccgcaa taaacgtcac gttccggctc aggctgctgt
      301 tgttgatgct ggcagtgcct gtactggttc cggctgtctg gctggtgata ctgatctcca
      361 ccgtgccgtc cggctctgta gtgaccgtcg gagacaccgt tgcgctgttg tctgccatca
      421 cgctgaccgt ctgcccggc
//
LOCUS       DAOTRK010000412          419 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.423, whole genome shotgun sequence.
ACCESSION   DAOTRK010000412 DAOTRK010000000
VERSION     DAOTRK010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.423"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..309
                     /locus_tag="QIE41_004935"
     CDS             <1..309
                     /locus_tag="QIE41_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HDS3533671.1"
                     /translation="YWIFFLISLIKFPETAEELLKNALVTLPADANYKDKTLLLKAIY
                     SGCTNLPFSLFINNEQLLEIRECCKQAIKVTFAAELFDTQNSNKKQNKKPWKKVMFNV
                     "
BASE COUNT          152 a           67 c           71 g          129 t
ORIGIN      
        1 tattggatat tttttctgat atcgttaata aaatttcctg agactgcaga ggaactcctg
       61 aaaaatgcac tggtaaccct acccgcagat gctaattata aagacaaaac gttactcctg
      121 aaggcgattt acagcggatg tacaaatctt ccattttcac tttttattaa taatgaacaa
      181 ttattagaga ttagggagtg ttgtaaacag gcaataaaag taacatttgc agcagagctc
      241 tttgatacac aaaacagtaa taaaaaacaa aataaaaaac catggaagaa ggtaatgttc
      301 aatgtttaac aaagttttga tagggttaag gagtcatcca gaattaataa ttcttggatt
      361 aatggtgatg attatcgcca tgctcataat tccattgccc acctacttaa tcgattttt
//
LOCUS       DAOTRK010000413          403 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.425, whole genome shotgun sequence.
ACCESSION   DAOTRK010000413 DAOTRK010000000
VERSION     DAOTRK010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.425"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>403
                     /locus_tag="QIE41_004936"
     CDS             <1..>403
                     /locus_tag="QIE41_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDS3533672.1"
                     /translation="VAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNLI
                     KAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGNL
                     TLISGSKNSEAQHYDFIKKKSIYEKLNSKSSF"
BASE COUNT          148 a           65 c           70 g          120 t
ORIGIN      
        1 ggtagcaata aataatgata tgccttttga ttctgtgata aatcagataa accaacatgc
       61 agataattca ggttttatag ctgctctgga tgaagattta tatgagcctc gtccgaatca
      121 agtgaactta ataaaagcaa ttttgcttcg cttagacatg gagcaacaag atgagagcgt
      181 gattaaaacc tatacgggga gaatcacaat tgagcatatt cttcctcaag cattagttaa
      241 tgaatactgg attaatagat tccaacctca agagcatgtc tactggctgc ataaaatagg
      301 taatttaaca ctcataagtg gttccaaaaa ttctgaagca caacactatg attttatcaa
      361 aaagaaatct atttatgaga aacttaatag taaaagctct ttt
//
LOCUS       DAOTRK010000414          394 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.427, whole genome shotgun sequence.
ACCESSION   DAOTRK010000414 DAOTRK010000000
VERSION     DAOTRK010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.427"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>394
                     /locus_tag="QIE41_004937"
     CDS             <1..>394
                     /locus_tag="QIE41_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfate sulfotransferase N-terminal
                     domain-containing protein"
                     /protein_id="HDS3533673.1"
                     /translation="FDKYRKTLVAGAVAITLGLSAPGVMAAGFKPAPPAGQLGAVIVD
                     PYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQK
                     FANKVTVEWKENGKVMKDDYVVHTSAIVN"
BASE COUNT          107 a           81 c          106 g          100 t
ORIGIN      
        1 gtttgataaa tatagaaaaa ctctggtagc cggagctgtg gcgataactc tgggtttgtc
       61 agcaccgggg gtgatggcag caggttttaa acccgcacca cctgccgggc aactgggtgc
      121 ggtcattgtc gatccctacg gcaatgcacc actgaccgct ttggttgact tagatagcca
      181 tgtcatttct gatgtcaaag ttaccgtcca tgggaagggc gaaaaaggcg tagaaatcag
      241 ctatcccgtg ggtcaggaat cactaaaaac ttatgatggt gtaccgattt ttggtcttta
      301 tcagaaattt gctaacaaag tgaccgttga gtggaaagaa aacggcaagg tcatgaaaga
      361 tgattatgtg gtgcacactt cggccatcgt caat
//
LOCUS       DAOTRK010000415          378 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.430, whole genome shotgun sequence.
ACCESSION   DAOTRK010000415 DAOTRK010000000
VERSION     DAOTRK010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.430"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>378
                     /locus_tag="QIE41_004938"
     CDS             <1..>378
                     /locus_tag="QIE41_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDS3533674.1"
                     /translation="GHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGGLT
                     RSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSED
                     EGLVTHWHYDEADRLTHRTVKGEP"
BASE COUNT           94 a          119 c          110 g           55 t
ORIGIN      
        1 gggccatgaa acgcggtatg aatacaacgc cgccggtgac ctgaccgccg tcattgcccc
       61 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatcc gtaccacaca
      121 gggcggtctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag
      181 tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac
      241 cggctttgac ggccgcacac agcgttatca ccacgacctg accggtaaac ttatccgcag
      301 cgaggatgag gggctggtca cccactggca ctatgacgaa gcagaccgcc tcactcaccg
      361 caccgtgaag ggtgaacc
//
LOCUS       DAOTRK010000416          377 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.431, whole genome shotgun sequence.
ACCESSION   DAOTRK010000416 DAOTRK010000000
VERSION     DAOTRK010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.431"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>377
                     /locus_tag="QIE41_004939"
     CDS             <1..>377
                     /locus_tag="QIE41_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDS3533675.1"
                     /translation="RSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFT
                     ARGGTLAGTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTL
                     TVSNTTLTQKAVNLNEGTLTLND"
BASE COUNT          101 a          111 c          110 g           55 t
ORIGIN      
        1 cggagcgacg gaaaggcatt cagtatcgga ggcggtcagg cggatgccct gatgctggaa
       61 aaaggcagtt cattcacgct gaacgccggt gatacggcca cggataccac ggtaaatggc
      121 ggactgttca ccgccagggg cggcacactg gcgggcacca ccacgctgaa taacggcgcc
      181 atacttaccc tttccgggaa gacggtgaac aacgataccc tgaccatccg tgaaggcgat
      241 gcactcctgc agggaggctc tctcaccggt aacggcagcg tggaaaaatc aggaagtggc
      301 acactcactg tcagcaacac cacactcacc cagaaagccg tcaacctgaa tgaaggcacg
      361 ctgacgctga acgacag
//
LOCUS       DAOTRK010000417          371 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.432, whole genome shotgun sequence.
ACCESSION   DAOTRK010000417 DAOTRK010000000
VERSION     DAOTRK010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.432"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            24..100
                     /locus_tag="QIE41_004940"
     tRNA            24..100
                     /locus_tag="QIE41_004940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="QIE41_004941"
     tRNA            143..218
                     /locus_tag="QIE41_004941"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT          106 a           79 c           88 g           98 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacc gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaatg aaagttgttc gtgagtctct caaattttcg
      361 caactctgaa g
//
LOCUS       DAOTRK010000418          360 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.434, whole genome shotgun sequence.
ACCESSION   DAOTRK010000418 DAOTRK010000000
VERSION     DAOTRK010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.434"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..108
                     /locus_tag="QIE41_004942"
     CDS             <1..108
                     /locus_tag="QIE41_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HDS3533676.1"
                     /translation="KNNNMRYFIAVIIVILSGLMLFITLRAVSIVGQGL"
     gene            110..>360
                     /locus_tag="QIE41_004943"
     CDS             110..>360
                     /locus_tag="QIE41_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS3533677.1"
                     /translation="MNVAILLSTYNGEKYLEEQLDSLLLQSYQDFVVYIRDDGSSDRT
                     VNIINQYVMKDNRFINVGNSENLGCAASFINLLRNASAD"
BASE COUNT          120 a           43 c           67 g          130 t
ORIGIN      
        1 aaaaataata atatgcgtta ttttattgca gtcattatag ttatcctttc aggcttaatg
       61 ttgtttataa cactaagggc tgtatcaata gttggtcaag gattataaaa tgaatgttgc
      121 tattttgttg tctacgtata atggcgaaaa atatttagag gaacaactgg attcattgct
      181 gcttcaaagt tatcaggatt ttgtagtgta tatccgtgat gacggatcat ctgatagaac
      241 tgtaaatata ataaaccaat acgtaatgaa agataacaga tttattaacg tgggtaattc
      301 agaaaatctt ggttgtgctg cttcgtttat taatttatta agaaatgctt cagccgatat
//
LOCUS       DAOTRK010000419          355 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.435, whole genome shotgun sequence.
ACCESSION   DAOTRK010000419 DAOTRK010000000
VERSION     DAOTRK010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.435"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>355
                     /locus_tag="QIE41_004944"
     CDS             <1..>355
                     /locus_tag="QIE41_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDS3533678.1"
                     /translation="RRDLWKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQL
                     AALGDKVTYQERLNALAQQADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQ
                     YGDNPLALNNVMSEQK"
BASE COUNT           95 a           89 c          122 g           49 t
ORIGIN      
        1 cgccgggatt tgtggaaggc ggagagtcag ttcgcggtac tggaggaggc ggcgcaacgt
       61 cgccagctgt ctgcacagga gaaatccctg ctggcgcata aagatgagac gctggagtac
      121 aaacgccagc tggctgcact tggcgacaag gttacgtatc aggagcgcct gaacgcgctg
      181 gcgcagcagg cggataaatt cgcacagcag caacgggcaa aacgggccgc cattgatgcg
      241 aaaagccggg ggctgactga ccggcaggca gaacgggaag ccacggaaca gcgcctgaag
      301 gaacagtatg gcgataatcc gctggcgctg aataacgtca tgtcagagca gaaaa
//
LOCUS       DAOTRK010000420          354 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.436, whole genome shotgun sequence.
ACCESSION   DAOTRK010000420 DAOTRK010000000
VERSION     DAOTRK010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.436"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(75..323)
                     /locus_tag="QIE41_004945"
     CDS             complement(75..323)
                     /locus_tag="QIE41_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533679.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVLMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          117 a           74 c           58 g          105 t
ORIGIN      
        1 ataacgcctg aaatgctcac tttgcaccag catgatgata cagctgatgt ttattctata
       61 accttactca agttctaaga gagcactgat tccctgtcat taataatgat atccgttagt
      121 gtagtcttat gcatttcaat gtactgcaag atttgcagat agagatcaga aagaatcaca
      181 ctagtttcat cttttaaacg caagcgtaaa gcctgatcaa taatatgact atcaacatac
      241 ccgtgcgtca tgagcacctc cctctcgaga tttttaaaca aagcacattg aaaaataaat
      301 ctctcgggga ttgaattatt catgtcagcc tcgtaggaat attgacccaa taaa
//
LOCUS       DAOTRK010000421          350 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.437, whole genome shotgun sequence.
ACCESSION   DAOTRK010000421 DAOTRK010000000
VERSION     DAOTRK010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.437"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>350
                     /gene="wzx"
                     /locus_tag="QIE41_004946"
     CDS             <1..>350
                     /gene="wzx"
                     /locus_tag="QIE41_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157658977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O1 family O-antigen flippase"
                     /protein_id="HDS3533680.1"
                     /translation="VIVGVYAAAEKIVKAVLSLFTPLTQAIYPYNCRKFSLSVFDGIE
                     AAKKTGIPIIILAFIAAVIVAITLPVAIDYLNFPKETIFVGQILSAWIFFGVLNNVFG
                     IQILSASGRSKIYS"
BASE COUNT          105 a           57 c           64 g          124 t
ORIGIN      
        1 ctgttattgt tggtgtatat gccgccgctg aaaagatagt caaggccgta ttgtcgctat
       61 ttacaccact gacgcaagct atatatcctt ataattgtcg taagttttca ctatccgtat
      121 ttgacggcat tgaggcagca aaaaaaactg gtataccaat tataatttta gcatttatag
      181 ctgctgttat cgttgcaatt accttacctg ttgcaatcga ctatcttaat tttccaaaag
      241 aaacaatttt tgtaggtcaa atattaagtg catggatctt ttttggtgtt cttaataatg
      301 tattcggcat tcagatattg agtgcatcag gaagaagtaa aatatatagt
//
LOCUS       DAOTRK010000422          336 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.438, whole genome shotgun sequence.
ACCESSION   DAOTRK010000422 DAOTRK010000000
VERSION     DAOTRK010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.438"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(33..185)
                     /gene="hokA"
                     /locus_tag="QIE41_004947"
     CDS             complement(33..185)
                     /gene="hokA"
                     /locus_tag="QIE41_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDS3533681.1"
                     /translation="MPQKYRLLSLIVICCTLLFFTWMISDSLCELHIKQGSYELAAFL
                     ACNLKE"
BASE COUNT          106 a           78 c           65 g           87 t
ORIGIN      
        1 gaaaggcggg aataacttcc cgccgaagac tcttactctt tcaaattgca ggctaaaaat
       61 gccgccagct cataactccc ctgtttaata tgcaattcac acagtgaatc acttatcatc
      121 caggtgaaaa ataaaagcgt gcaacaaatc actattaaag aaagtaatct atatttctgc
      181 ggcattccca gccccctgtg ttgatttcca acgagtaaga ggctaacctt atggtgtcta
      241 tgcatagagg ggcctcactt tgtactgacc ccaaaaagtt ggacagttaa acacgaggca
      301 tataggtctg attccgatat tcaattggag tcagac
//
LOCUS       DAOTRK010000423          332 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.440, whole genome shotgun sequence.
ACCESSION   DAOTRK010000423 DAOTRK010000000
VERSION     DAOTRK010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.440"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            47..271
                     /gene="tnpA"
                     /locus_tag="QIE41_004948"
                     /pseudo
     CDS             47..271
                     /gene="tnpA"
                     /locus_tag="QIE41_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT           94 a           69 c           73 g           96 t
ORIGIN      
        1 tggtcttaac tggaatttaa atgcctgtaa tcgtttcaca ggtcaaatga tactttggtc
       61 tatgaaaaaa gaaaccgata tccggcgtgg aaggcattgt gttttcctga tgcatgttca
      121 cctggtcttt gtcaccagat accgacgcca gatttttgac catgacgcga cagaaaaact
      181 acgcacttac ttttcaaatg tatgtgctga ttttgaagct gaactggttg aaatggatgg
      241 cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga tatccagtct
      301 ggtaaacagc ctcaaaggcg tatcgggtag gt
//
LOCUS       DAOTRK010000424          316 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.442, whole genome shotgun sequence.
ACCESSION   DAOTRK010000424 DAOTRK010000000
VERSION     DAOTRK010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.442"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            3..>316
                     /locus_tag="QIE41_004949"
     CDS             3..>316
                     /locus_tag="QIE41_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDS3533682.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPH
                     FLEHVGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHD
                     VF"
BASE COUNT           99 a           59 c           70 g           88 t
ORIGIN      
        1 caatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
       61 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
      121 accttcttcc acactttctg gaacatgtag gtaacaacca tctggatatt ggtgttggaa
      181 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
      241 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
      301 gccatgatgt ttttga
//
LOCUS       DAOTRK010000425          316 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.443, whole genome shotgun sequence.
ACCESSION   DAOTRK010000425 DAOTRK010000000
VERSION     DAOTRK010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.443"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
BASE COUNT           63 a          102 c           72 g           79 t
ORIGIN      
        1 gttgcatttc gctgattatc aaaggtcttc acctcgttag gtcagttaat tcacgtcgca
       61 aacacattca aactgcttcc cttcgccatg taatgggctt tccccatcgc ggactactac
      121 ggaagctccg ccagccagcg cgtcatcgga gccatgcccc cttaacatcc gtcgctgacc
      181 ttccccggtt tacctgcctg gactcaggca tactgaggag gctgcccgtc gcactcttta
      241 tccttgcttg ccgcaagttg gcagaagtca gcaacgcaag cgtgatagac gctgctgccc
      301 cggtgtttcg cataca
//
LOCUS       DAOTRK010000426          301 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.445, whole genome shotgun sequence.
ACCESSION   DAOTRK010000426 DAOTRK010000000
VERSION     DAOTRK010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.445"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>301)
                     /locus_tag="QIE41_004950"
     CDS             complement(<1..>301)
                     /locus_tag="QIE41_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HDS3533683.1"
                     /translation="KPDYNLRIMQPGQTLVSVATNILSRLSEVLIIEKPDIILVHGDT
                     TTTLAATLAGYYHQIKVCHVEAGLRTGDIYSPWPEEGNRKVTGALACIHFAPTERS"
BASE COUNT           85 a           64 c           55 g           97 t
ORIGIN      
        1 tgatctctct gttggggcga aatgaataca tgctaatgcc cctgtaactt tacgattgcc
       61 ctcttcaggc caaggagagt aaatatcccc tgttcttaat cctgcttcca catgacaaac
      121 ttttatttgg tggtaatacc cagctaaagt agcagcaagg gtagtcgttg tatccccatg
      181 cacaagtata atatctggct tttctataat taaaacttca cttaaccgtg agagtatatt
      241 tgttgctaca gatactaatg tttgcccagg ctgcataatc cgtaaattat aatcaggttt
      301 a
//
LOCUS       DAOTRK010000427          291 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.446, whole genome shotgun sequence.
ACCESSION   DAOTRK010000427 DAOTRK010000000
VERSION     DAOTRK010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.446"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..281)
                     /locus_tag="QIE41_004951"
     CDS             complement(<1..281)
                     /locus_tag="QIE41_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDS3533684.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKK"
BASE COUNT           57 a           75 c           83 g           76 t
ORIGIN      
        1 tgttttttac ctttgcactc cccaaactcc cgggcccaga cgtaatgcag ggttgtggga
       61 tgcacgcgtt taccttcccg atgcttacgt ttctggtaag cgtcgattcg ggctttcagg
      121 gcattgatga agcgggatat cacagctgcg tccgttgctg ccggtacatc cgggaaacgc
      181 aggtcaaccc gcaatgccgt caggcgggga tgaacattca gtgcgtgccg caccgtctca
      241 cgaatacgtt gctgccagaa ggggttgtat ttgtaggtca tggttaaatc t
//
LOCUS       DAOTRK010000428          275 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.448, whole genome shotgun sequence.
ACCESSION   DAOTRK010000428 DAOTRK010000000
VERSION     DAOTRK010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.448"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(99..174)
                     /locus_tag="QIE41_004952"
     tRNA            complement(99..174)
                     /locus_tag="QIE41_004952"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(139..141),aa:Thr,seq:ggt)
     gene            complement(189..>275)
                     /gene="rrf"
                     /locus_tag="QIE41_004953"
     rRNA            complement(189..>275)
                     /gene="rrf"
                     /locus_tag="QIE41_004953"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT           61 a           82 c           66 g           66 t
ORIGIN      
        1 cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccagac
       61 aaattctttt ctaatctgcc gaactttaac ctaaaaagtg gtgctgatac ccagagtcga
      121 actggggacc tcacccttac caagggtgcg ctctaccaac tgagccatat cagcacgcta
      181 aatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc
      241 tacggcgttt cacttctgag ttcggcatgg gatca
//
LOCUS       DAOTRK010000429          271 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.449, whole genome shotgun sequence.
ACCESSION   DAOTRK010000429 DAOTRK010000000
VERSION     DAOTRK010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.449"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>271)
                     /locus_tag="QIE41_004954"
     CDS             complement(<1..>271)
                     /locus_tag="QIE41_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533685.1"
                     /translation="QLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVLK
                     LDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVP"
BASE COUNT           55 a           93 c           73 g           50 t
ORIGIN      
        1 tccggcaccc gctcacacca gccgagcagc caccacggcc cctgcggact gtttgtcgcc
       61 agataacgat gcggacttaa gcggagttct tccggcagcg cctgccagag tgcggccagc
      121 cggtgacctt catccagttt caggacaccg ccacgcacca gccagagtga ctcgctgcgg
      181 ctgtaaccgt cctcaccggg aaacaggtgc tcaaaataca ggctcctgcc gccgttatca
      241 ctgagtatca gtgtgttatc gcgcagctgt a
//
LOCUS       DAOTRK010000430          270 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.450, whole genome shotgun sequence.
ACCESSION   DAOTRK010000430 DAOTRK010000000
VERSION     DAOTRK010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.450"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>270
                     /locus_tag="QIE41_004955"
     CDS             <1..>270
                     /locus_tag="QIE41_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS3533686.1"
                     /translation="ALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNTV
                     KNWIKGYRKSGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNEL"
BASE COUNT           80 a           63 c           68 g           59 t
ORIGIN      
        1 ggcactggat tgcagacaca agcgtagcta ttctcctgaa gataaacttt gcgtggttct
       61 ttatgctctc gggcattctg agtctctacc ccgggtagct gcccggttta acattcccag
      121 ccacaacacg gttaaaaact ggataaaagg ctaccgcaaa tctggtaatg aggcctttat
      181 caggcgcagg aaggagaaaa gcatgacccg ttctgatgat acccatgaaa acgaggcaaa
      241 catgacgccg gaagagatga agaatgagct
//
LOCUS       DAOTRK010000431          261 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.451, whole genome shotgun sequence.
ACCESSION   DAOTRK010000431 DAOTRK010000000
VERSION     DAOTRK010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.451"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>261
                     /locus_tag="QIE41_004956"
     CDS             <1..>261
                     /locus_tag="QIE41_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533687.1"
                     /translation="NGLILMKLPETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKN
                     ARILGDNNARNGADRLVSGTEIQTKYCSTAARSVGAAFDGQNG"
BASE COUNT           80 a           56 c           75 g           50 t
ORIGIN      
        1 aatggtctga ttctcatgaa actgccggaa acctggagta caaatgagaa aatgtttgct
       61 tccggagggc agggacatgg gtttgccgct gaacggggaa accatattgt cgacagagtc
      121 cgtctgaaaa acgcacggat cctcggtgat aataatgcca gaaatggagc agacagactg
      181 gtcagcggaa cagaaatcca gacgaaatat tgttcaactg cagcccgtag cgtcggtgcg
      241 gcattcgacg gacaaaacgg a
//
LOCUS       DAOTRK010000432          260 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.452, whole genome shotgun sequence.
ACCESSION   DAOTRK010000432 DAOTRK010000000
VERSION     DAOTRK010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.452"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>260)
                     /locus_tag="QIE41_004957"
     CDS             complement(<1..>260)
                     /locus_tag="QIE41_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDS3533688.1"
                     /translation="LYDWWVLGVSNWLAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGF
                     YLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVF"
BASE COUNT           77 a           62 c           48 g           73 t
ORIGIN      
        1 tcaaaaacat catggctaat tttatgttta atttttgatt ccccagccct tgtcgatgcc
       61 gcgttcaggc tagcttcgtt caaatccatt aaagatatca gactgctctc aggtacgtga
      121 gtaaggtaaa acccagttcc aacgccaata tccagatggt tgttacctaa atgttccaga
      181 aagtgtggaa gaaggtgttc ctttgtagga catccccatg caagccaatt tgatactccc
      241 aaaacccacc agtcataaag
//
LOCUS       DAOTRK010000433          256 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.454, whole genome shotgun sequence.
ACCESSION   DAOTRK010000433 DAOTRK010000000
VERSION     DAOTRK010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.454"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..>256
                     /locus_tag="QIE41_004958"
     CDS             <1..>256
                     /locus_tag="QIE41_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDS3533689.1"
                     /translation="AKANHYLDDADLPAVITLHGRFSQGWYFCFESQEFLESGDEAAR
                     LAGNAPFIIDKDSGEIHSLGTAKPLEEYLQVYEIKKATFGL"
BASE COUNT           69 a           56 c           60 g           71 t
ORIGIN      
        1 gcgaaagcga accattacct tgatgatgca gatctccctg ccgtcatcac tctgcatgga
       61 cgttttagcc agggctggta tttctgtttc gaatcacaag aatttcttga atctggagat
      121 gaggccgcgc gtttagccgg taacgcacct tttattattg ataaagacag tggtgaaatt
      181 cattcccttg gaacggcaaa gccgctggaa gaatatctac aggtttatga aataaaaaag
      241 gctaccttcg gcttgc
//
LOCUS       DAOTRK010000434          243 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.denovo.456, whole genome shotgun sequence.
ACCESSION   DAOTRK010000434 DAOTRK010000000
VERSION     DAOTRK010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.denovo.456"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(73..>243)
                     /locus_tag="QIE41_004959"
     CDS             complement(73..>243)
                     /locus_tag="QIE41_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS3533690.1"
                     /translation="GVFNFVGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALS
                     YILMVGDVKRVG"
BASE COUNT           75 a           68 c           58 g           42 t
ORIGIN      
        1 gtgagatggt tactgaatat ttctatctgt tcagcacagg tatgagaaaa aagggcttcc
       61 tcagataaga gattatccaa cgcgcttcac atcgcccacc atcagaatgt aagagagcgc
      121 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
      181 caggtacccc accaccagcg gaacggtgat gccgcccaga ccgccaacaa agttaaatac
      241 gcc
//
LOCUS       DAOTRK010000435        14191 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.guided.121, whole genome shotgun sequence.
ACCESSION   DAOTRK010000435 DAOTRK010000000
VERSION     DAOTRK010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.guided.121"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            complement(34..390)
                     /locus_tag="QIE41_004960"
     CDS             complement(34..390)
                     /locus_tag="QIE41_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDS3533691.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(383..661)
                     /locus_tag="QIE41_004961"
     CDS             complement(383..661)
                     /locus_tag="QIE41_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDS3533692.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(766..2478)
                     /locus_tag="QIE41_004962"
                     /pseudo
     CDS             complement(766..2478)
                     /locus_tag="QIE41_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            2450..3235
                     /gene="lafU"
                     /locus_tag="QIE41_004963"
     CDS             2450..3235
                     /gene="lafU"
                     /locus_tag="QIE41_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDS3533693.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISLSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3306..4361
                     /gene="dinB"
                     /locus_tag="QIE41_004964"
     CDS             3306..4361
                     /gene="dinB"
                     /locus_tag="QIE41_004964"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDS3533694.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            4413..4706
                     /gene="yafN"
                     /locus_tag="QIE41_004965"
     CDS             4413..4706
                     /gene="yafN"
                     /locus_tag="QIE41_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HDS3533695.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            4709..5107
                     /gene="yafO"
                     /locus_tag="QIE41_004966"
     CDS             4709..5107
                     /gene="yafO"
                     /locus_tag="QIE41_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HDS3533696.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            5117..5569
                     /locus_tag="QIE41_004967"
     CDS             5117..5569
                     /locus_tag="QIE41_004967"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS3533697.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            5815..6021
                     /locus_tag="QIE41_004968"
                     /pseudo
     CDS             5815..6021
                     /locus_tag="QIE41_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            6017..6369
                     /gene="prfH"
                     /locus_tag="QIE41_004969"
                     /pseudo
     CDS             6017..6369
                     /gene="prfH"
                     /locus_tag="QIE41_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(6426..7883)
                     /gene="pepD"
                     /locus_tag="QIE41_004970"
     CDS             complement(6426..7883)
                     /gene="pepD"
                     /locus_tag="QIE41_004970"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDS3533698.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            8144..8602
                     /gene="gpt"
                     /locus_tag="QIE41_004971"
     CDS             8144..8602
                     /gene="gpt"
                     /locus_tag="QIE41_004971"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDS3533699.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            8694..9938
                     /gene="frsA"
                     /locus_tag="QIE41_004972"
     CDS             8694..9938
                     /gene="frsA"
                     /locus_tag="QIE41_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDS3533700.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            9996..10397
                     /gene="crl"
                     /locus_tag="QIE41_004973"
     CDS             9996..10397
                     /gene="crl"
                     /locus_tag="QIE41_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDS3533701.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(10436..11491)
                     /gene="phoE"
                     /locus_tag="QIE41_004974"
     CDS             complement(10436..11491)
                     /gene="phoE"
                     /locus_tag="QIE41_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDS3533702.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            11779..12882
                     /gene="proB"
                     /locus_tag="QIE41_004975"
     CDS             11779..12882
                     /gene="proB"
                     /locus_tag="QIE41_004975"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDS3533703.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            12894..14147
                     /gene="proA"
                     /locus_tag="QIE41_004976"
     CDS             12894..14147
                     /gene="proA"
                     /locus_tag="QIE41_004976"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDS3533704.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESSVALHADVAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         3460 a         3568 c         3669 g         3494 t
ORIGIN      
        1 aaaaggtcgg atgccacgcg gcatccgacc tgtttacatc tgggctgaaa actcacgttc
       61 taagacatca acaatgagct ggttttttga aatggaatgt tgtctggcga cgatggtcag
      121 ccgcgaatca atgtgatgag gaacacgaag cgtcaggcgt ttttgctctt cttcttcacg
      181 aaaaggggca ataccgtcct cttcacaatc cgccatccac tcatccagcg cgctttcgcc
      241 ttcacgatat aatgcctcaa tacttgtggc tacaaagtca caataaccgt ttacattgag
      301 gaacttgccg cgaaatgcgt tgatatcggc ttcaaatgtc actgcggctg gttgaccttt
      361 aattgtgata atgttcgatg ctttattcat aatctacccc tacacagtta acaaaccact
      421 ccttaagacc tgctactgcg cctttatcca tcacattctt tggatgtggc tcatgtgtgc
      481 tgaatttggt cgttccaatc tgaaattttc gtctggagcc gttgttattt tttattgttc
      541 cacccacagc tttaatgagt gcaacaacat catcccattt aattccagat tttggcggct
      601 ccgtcattac atcgtcgaat gtttttctat gacgagagcc tagcgcgctg accttaatca
      661 tactatcaat ccatgacgtc attgtatggt gtcatcgtag tctctttgca gtgtgacgtc
      721 aattaatggt gtcaggtgct gcaaagcatg gaaaagattc ctcaatcaac tcaacgcccc
      781 cataatcttc aactccagct cgtccggcac ttcgttatac gacagcacat gcagccccgg
      841 cgcaaacaac cttgcataac gcgccagcaa aggacgcaac tgcggcggca ccagcagcac
      901 cgggtctttc cccgccgctt tcatctgctc ttttacctgc ggcatcgtac tctggaactg
      961 gttgagcata ttcggatcga ccggcacgct gtcgagcatc actttcccgc cctgttgcgc
     1021 ctgattcacc acgttggtga gcagattttc cagctcatta ttcagcgtat acaccgtcag
     1081 ctcctgcttg cgaacgaacg gatgggtaat gctgcgccgc aatgccaggc gcacatcggc
     1141 cgccagcagg atatgatctt tggtcactgt gctactggcg accagcacgg tggcgatggt
     1201 gacaatatcg cgcagggaaa cgccttcggt cagcagcgcc cggtacactt tcagcaattg
     1261 gctgtaattg agcgccgcgc tcaaatcttc cgccaggcgc ggtgccatcg acgacaaacg
     1321 gttatgcaac tgcgtgatgt catcatagtt aaacaaatca ggaatatagc tgcgcacaat
     1381 cttgttcaca tgggtagcga tcacgctggc gctgtcgatc acctgatacc ccatattcag
     1441 cgctttcgct ttctgcgcgg gctggatcca ggtcaccggc atcccgtacg ccgggtcgtt
     1501 ccccagcacg ccgtcaatct cgccgtaggt ttcgctggag ggcagggcca tcagtttatc
     1561 cgccggaata tccgcttcat cggccttaat gccgttgatg aatatggcgt actggctggg
     1621 cttgaggcgg aagttttccc gaatacggat ctccggcagc agcacgccgt tgccgtcgga
     1681 tatcacttgc cgcacgccgc gaatacgctg ggtgagtggg ttaccctgcg ctttatcgac
     1741 cagcgccacc agtttgtaac cgaggcttaa actgatgggt tcgatcagcg gaatagtctc
     1801 ccaactgacc tgttgctcgc tggtttcggt gatggtgcgg gtcagcgttt cgaggctttt
     1861 ctcctccgcc tccgccgcct gcgggcgttt gctcatccgc cagccggtaa aaccaagcag
     1921 ggcgctgaac agcaaaaacg gcagatgcgg catccccggc acaaccgcca gcacaaacat
     1981 gatcccggtg gcggtgtaga gcaccgacgg gcttgccagc aactggttac gcacgtcatg
     2041 ggcaatatcg ccgttgtcgc tgacgcgggt gacgataatc gccgccgcgg tggaaagcag
     2101 cagggacggg atctgcgcca ccaggccgtc gccgatggtc atcagcacat attgctggaa
     2161 ggcggcatcg gcgctcaggt tgtatttgaa gatcccgata cagacgccgc cgatcaggtt
     2221 gatcgccaga atcatcatcc cggcgatggc atccccgcgc acaaacttcg atgccccgtc
     2281 cattgcgccg tagaaatcgg cctcgctggc aacatcttta cgccgggttt gcgcctgcgc
     2341 ctggttgatc aacccggcgt taagatcggc gtcaatcgcc atctgtttgc cgggcatcgc
     2401 gtccagggta aagcgggcag aaacctcgga aatacgctcg gcccctttgg tgacgacgat
     2461 aaagttgatt gtcaacagcg tcagtaaatc cgagcgtgaa agcattatcg ccgcgctgca
     2521 cgggcagtcg attttcaatg gcggcgggtt gtcgccgtta aataaaatca gcctgtctca
     2581 tccgccaaaa cctgccaccg tggcggtgcc ggaagagacg gagaaaaaag cgcgtgacgt
     2641 caatgagaaa acggcgctgc tgaagaagaa atccgctact gagcttggcg agctggcgac
     2701 cagtatcaac accattgccc gtgatgcaca tatggaagcg aatctggaga tggagattgt
     2761 tccccaggga ctgcgcgtgc tgattaaaga tgaccagaac cgcaatatgt ttgaacgcgg
     2821 cagcgcacag attatgccgt tctttaaaac tttgctggtg gagctcgcgc cagtgttcga
     2881 ctcgctcgat aacaaaatta ttattaccgg gcataccgat gcgatggcct acaaaaacaa
     2941 tatctacaac aactggaacc tttcgggcga tcgcgcgctt tcggctcgtc gggtgctgga
     3001 agaggccgga atgccggaag ataaagtgat gcaggtgagc gcgatggcgg accagatgct
     3061 gctggacgcg aaaaatccgc aaagcgcggg caaccgacgc attgagatta tggtgctgac
     3121 caaaagtgcg tccgatacgc tgtatcaata ctttggtcag catggggata aagtggtgca
     3181 gccgctggtg caaaagctgg ataagcagca ggtgctttcg cagcgaacgc gctagatgct
     3241 gaatctttac gcatttctca aaccctgaaa tcactgtata ctttaccagt gttgagaggt
     3301 gagcaatgcg taaaatcatt catgtggata tggactgctt tttcgccgca gtggagatgc
     3361 gcgacaatcc cgccctgcgc gatatcccta ttgctattgg cggcagccgc gaacgtcggg
     3421 gggtgatcag caccgccaat tatcccgcgc gtaaatttgg cgtacgtagc gctatgccta
     3481 ctgggatggc gctcaaatta tgcccacatc tcaccttgct tccggggcgc tttgacgcct
     3541 acaaagaagc ctcaaatcat atccgtgaaa tcttctcgcg ctacacctcg cgcattgaac
     3601 cgttgtcact ggatgaggcc tatctcgatg tcaccgatag cgtccattgc cacggttctg
     3661 cgaccctcat cgcccaggaa atccgccaga cgattttcag cgagctgcaa ctgacggcgt
     3721 ctgcgggcgt ggcaccagta aagtttctcg ccaaaatcgc ctccgacatg aataaaccca
     3781 acggccagtt tgtgattacg ccggcagaag ttccgacatt tttacaaacc ttaccgctgg
     3841 caaaaatccc cggcgtcggc aaagtctcgg cggcaaaact ggaagcgatg gggctgcgga
     3901 cctgcggtga tgtgcaaaag tgtgatctgg tgactctgct caaacgtttt ggcaaatttg
     3961 gccgcatttt gtgggagcgt agtcagggga ttgacgagcg cgacgttaac agcgaacggt
     4021 tgcgaaaatc cgtcggcgtg gaacgcacga tggcggaaga tattcatcac tggtctgaat
     4081 gtgaagcgat tatcgagcgg ctgtacccgg aacttgaacg ccgtctggca aaagtaaaac
     4141 ctgatttact gattgctcgc cagggggtga aattaaagtt tgatgatttt cagcaaacca
     4201 ctcaggagca cgtctggccg cggctgaata aagctgactt aattgccacc gcgcgtaaaa
     4261 cctgggatga acgtcgcggc gggcgcggtg tgcggctggt ggggctgcat gtgacgttgc
     4321 ttgacccgca aatggaaaga caactggtgc tgggattatg atgtatacta ttatgtatat
     4381 tctggtgtgc attattatga gggtatcact gtatgcatcg aattctcgct gaaaaatcgg
     4441 tcaatatcac tgagttacgt aaaaatccag caaaatactt tattgatcaa ccggttgcgg
     4501 ttctttctaa taatcgcccc gcaggatatc tcttaagtgc cagcgcattc gaagcgttaa
     4561 tggacatgct tgcagaacaa gaggagaaaa aactggtgaa ggcgcgcttc cgtccgagtg
     4621 ccgcaagatt agaggaaatt acacgccgcg ctgaacaata tcttaatgat atgacggatg
     4681 atgatttcaa tgactttaag gaataaggat gcgggtattc aaaacaaaac ttattcgcct
     4741 gcaacttaca gcagaggaac ttgatgcgtt aacggcggat tttatttcct ataagcgtga
     4801 cggtgttttg ccagatatat ttggtcgcga tgcactctac gacgactcgt ttacctggcc
     4861 attaatcaaa tttgagcgag ttgctcatat tcatctggca aatgtgaata atccatttcc
     4921 gccacagttg cgccaattca gcagaacgaa tgacgaagcg catttggtat attgtcaggg
     4981 tgcgtttgat gagcaagcat ggttgctcat tgccattctg aagcctgaac ctcataaact
     5041 ggctcgagat aacaaccaaa tgcataaaat tggaaaaatg gcagaagcgt ttcgcatgcg
     5101 tttttgaatt tatattatga ataacataca aataagagat tatcagcctg gcgattttca
     5161 gcaactatgc gctattttca ttagagcggt tacgatgacc gccagtcagc attattcacc
     5221 acaacaaatt gccgcctggg cgcagattga cgaatctcgc tggaaggaga aactcgcgaa
     5281 atcacaagtg cgggttgcag tcattaatgc aaaactggtc ggctttatta cctgcgttga
     5341 acattatatc gatatgttat ttgttgaccc tgaatacacc cgccgtgggg ttgccagcgc
     5401 tttgttaaaa cctttgatta agtctgaatc cgaactaacg gtagatgcca gcataaccgc
     5461 aaaacccttt tttgaacgtt atggttttca gacagttaag cagcagcgcg ttgaatgccg
     5521 gggagcgtgg tttactaatt tttatatgcg atataaaccg caacattaaa tccagcttgc
     5581 aatgaaaata acgcccgcct agtatgtgcg cgttttctta ttccacaaac tgcaaggagg
     5641 taaatcatga caaatccttt atcaatgact ctttgcagac ctttccagga ttaattcttt
     5701 ttttcttgcc ctggattcgt ctgccatttc ctgattttta tatttatata ctctaaataa
     5761 ttcgagtcgc agtacatgca acttgaagta tgacgagtat aaatggaatg atacatgggc
     5821 aaatatattc gtcccttatc cgatgcggta tttaccatcg catctgatga cctgtggatc
     5881 gagagtttag cgatccaaca attacacacc acggcaaatt tacccaacat gcagcgcgta
     5941 gtagggatgc cagatttaca ccccggacgc ggctacccga ttggcgcagc gttattctct
     6001 gttggtcgtt tttacccggc aagacgtcgc ggtaacggtg ctggaaacag aaacgggccg
     6061 ctgctctgac acgctgcgtt cggcgctgat ttctctggat ggcgataacg catgggcatt
     6121 aagcgaaagt tggtgcggca ctattcagtg gatttgtccg agtccgtatc ggcctcatca
     6181 tgggcgcaaa aacttgtttc tgggcatgct aacaagcggc tggcgcgatt gctgattgcc
     6241 tggaagctgg aacaacagca acaggaaaat agcgcggtgc tgaaatcgca gcggcgaatg
     6301 ttccatcacc agattgaacg tggcaacccg agacggacat ttacagggat ggcttttatc
     6361 gaaggataat gaaggatgaa actgccggaa ggcgattaaa cgccatccgg cagcaaatca
     6421 aataattact tcgccggaat ttctttcagc agttcagtca gcagtgtcca gtaatgacct
     6481 acgctttcga tgtgaacttg ctcatccgga gagtgtggac cggtgattgt tggcccgata
     6541 gaaaccatgt ccatttccgg atacggtttt ttaaacagac cacattccag gcccgcgtga
     6601 ataatctgga tgttcggcgt cttgttgaac agtcgctgat aggtttcacg taccagatgc
     6661 atcaccggag aattagcgtc cggctgccag ccaggatatg cgcctttcgc ttcggttttc
     6721 gcgccagcca gtttacccag cgaatccagc atgctcacca cgtagtcttt accgctgtcg
     6781 atcagagaac ggatcaggca gtgaatttct acgttattgt cagtcatggt caccacaccg
     6841 acgttcaggg aggtttcgac cacacctttt gccacgtcgg agttgcggat cacaccgttc
     6901 ggggtggcgt tcagcagacg aataaaggta tcgcgagatt tcgcaatcag ggcagcttta
     6961 tcgttcgcaa cagagtccag cagcaatgcc agatttttct ctttctctgc cagctcgttt
     7021 ttcaggattt cctgataggt attcaccaga gatttcaggg cgtcgacttt atcagctgcg
     7081 acagcaatgg tcgcaaaggc ttcacgcggg atggcgttac gcagtgtgcc accgttgaaa
     7141 tcgataaggc gcaggtccag ctcttctgca tgacccgcca ggaagcgcac cagcagtttg
     7201 ttggcattac ccaggccaac gtggatttca ccgccggagt gaccgccttt tagacctttt
     7261 aaggttaact tgaaggtttc aaaaccagct ggaactgctt cacgatctaa atgcaggttg
     7321 gaggtgaagt cgataccccc cgcgcaaccc atgtagattt caccttcctc ttcggagtcg
     7381 gtgttaatca gaatatcagc ctgcaaccag ttgctctgta agccaaaagc accatccata
     7441 ccggcttctt cggtcatggt cagcagcact tccagcgggc cgtgaaccac gttttcgtca
     7501 gccagaaccg ccagcgcaga cgccatacca atgccgttat ccgcacccag cgtggtgccg
     7561 cgcgctttaa cccattcgcc atcaatataa ggctggatag gatctttagt gaagtcatgc
     7621 acggtgtcgt tatttttctg tggcaccata tcgaggtggg cctgcaagac gaccggttta
     7681 cgattttcca tacctgcggt agcaggttta cgaatcagga tattacctac ctgatcgcgt
     7741 tcgacatgga aacctttctc ttttgcccaa ccaacaatgt attcagcgag ttgctcttca
     7801 tgataggacg ggtgaggaat agaacagatt ttggcaaaaa tatcccacag cggctgtgga
     7861 gataattgag acagttcaga cacgttaagt ctccttgtcg atcacccgca aaacagtatt
     7921 gcaggtcaca gggttagcag aaaatgttgt caacacaaga caggcttgcg agatatgttt
     7981 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     8041 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     8101 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     8161 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     8221 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gtgcgttact
     8281 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     8341 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     8401 tattgatgac ctggtggata ccggtggcac tgcggttgcg attcgtgaaa tgtatccaaa
     8461 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     8521 tgttgatatc ccgcaagata cctggatcga acaaccgtgg gatatgggcg tcgtattcgt
     8581 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     8641 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     8701 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     8761 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     8821 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     8881 cacgtgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     8941 atgatttata cgatacggtg attggctacc gtggtggcaa ctggatttat gagtgggcca
     9001 cccaggcgat ggtgtggcaa caaaaagcct gtgcggaaga agatccgcaa ctcagtggtc
     9061 gtcactggct gcatgcggct acgttgtaca acattgccgc ctatcctcat ctgaaaggag
     9121 atgacctggc cgagcaagcg caggctttgt caaaccgcgc ctatgaagag gccgctcagc
     9181 gtctgccggg caccatgcgg cagatggagt ttaccgtacc cggcggtgcg cccatcaccg
     9241 gctttttgca tatgccgaaa ggcgatggcc cgttcccgac agtattaatg tgtggtggtc
     9301 tggatgcgat gcagacggac tattacagcc tgtatgaacg ttattttgcg ccgcgcggca
     9361 ttgcgatgct gactattgat atgccgtcgg tgggcttttc ttcaaaatgg aagctcaccc
     9421 aggactccag cctgttgcat cagcacgtct taaaggcgct gcctaacgta ccgtgggtag
     9481 atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg
     9541 cataccttga atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta gttcataccc
     9601 tgttgagtga ttttaagtgc cagcaacagg tgccggaaat gtatcttgac gttctggcga
     9661 gccgtttggg gatgcatgat gcttccgacg aagcgttgcg cgtggagctg aatcgctatt
     9721 cattaaaagt gcaaggattg ctgggacgtc gctgcccaac gccaatgtta tcaggctact
     9781 ggaagaacga tccgttcagc ccggaagagg actcacgctt aatcacctca tcatctgctg
     9841 acggtaaatt gttagagatc ccatttaacc cggtgtatcg gaattttgac aaaggtcttc
     9901 aggaaataac agactggatt gaaaaacgat tgtgttaaaa atttgctaaa ttttgccaat
     9961 ttggtaaaac agttgcatca caacaggaga ttgcaatgac gttaccgagt ggacacccga
    10021 agagcagatt gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa ggtaagtgcg
    10081 aagataatcg attctttttc gattgtctgg ctgtatgcgt caacgtgaaa ccggcaccgg
    10141 aagtgcgtga gttctggggc tggtggatgg aactggaagc gcaggaatcc cgttttacct
    10201 acagttacca gtttggtctg ttcgataaag caggcgactg gacgagtgtt cagataaaag
    10261 atgccgaagt ggttgaacga ctggagcaca ccctgcgtga atttcacgag aagctgcgtg
    10321 agctgctggc gacgctgaat ctgaagctgg aaccggcgga tgattttcgt gatgaaccgg
    10381 tgaagttaac ggcgtgagtg aaatgtgccg gatgcatcac atccggcaat atccattaaa
    10441 actgataggt catgccaacc gcgacaatat catcattatt gatgttcagc ttattatcgc
    10501 tatccagttg gttgatttta taatcaacaa acgctgacat atttttgttg aaataatatg
    10561 tagcaccgac gtcgatataa ttgaccagat cttcatcacc gatgccttca atatctttcc
    10621 ctttcgataa gacataaccc agcgacggac gcagaccaaa gtcaaactgg tattgggcga
    10681 ccgcttcaaa gttctgtgtc ttattggcaa agccgccagt tattggcgtc attttgcgtg
    10741 tttcagaata gaaagttgcc agataaatat tattggcatc gtatttcaga cctgttgccc
    10801 atgcttctgc acgcttgcct gtgccacggc tttgcaggtt ctgctcgtta gtgcgatcgg
    10861 agttggtata ggccccactg atggcgaaat cgctgccacc aaagtcatat gtcaatgacg
    10921 taccgaagcc atcgccgttt tgctttttaa cgtcgcggtt ttcgtttttc ccttgatatt
    10981 gcagggttaa gttcaggcca tcgataacgc cgaagaagtc ggtgttccga tacgtcgcca
    11041 gaccgctggc gcgtttggtc ataaagttgt cggtctgcgc cgaggagtcg ccaccaaatt
    11101 ccgggaacat atcggtccag gcttccacgt catacagcgc gcccaggtta cgaccataat
    11161 cgaaagaacc caaatcttta tatttcaacc cggcaaaagc gagacgcgtt ttttgctgtg
    11221 cagtatcact ctccgcttta ttaccggcaa actctgcttc ccaacgacca taacccgtca
    11281 gttgatcgtt aatttgtgtt tcgcctttaa aaccaaaacg gatataactc tggtcgccat
    11341 ctttactgtc gttatcactc atataatgca tggctttaac tttgccatag acatccagtt
    11401 tattaccgtc tttgttatat atttctgcgg cctgtacgga tgcagatgcc acaatgccca
    11461 tcaccactaa tgccagagtg ctctttttca ttttcattcc tgattttaat taacgcgcga
    11521 atattcagcg ggagagtccc gttgaaaaca ggaaagtttt taacctgaga ttgttaaaga
    11581 tatattacag attaataata ttcttaaaat gtggtaattt attaaatctg taataaaagc
    11641 gtaaacaact gccgctacgc ttgctgatcc cgcgcaacaa aacgccatgc tttgctcgca
    11701 gatggttggc aaccgacgac agtcctgcta aaacgttcgt ttgatatcat ttttcctaaa
    11761 attgaatggc agagaatcat gagtgacagc cagacgctgg tggtaaaact cggcaccagt
    11821 gtgctaacag gcggatcgcg ccgcctgaac cgtgcccata tcgttgaact tgttcgccag
    11881 tgcgcgcagt tacatgccgc cgggcatcgg attgttattg tgacgtcggg cgcgatcgcc
    11941 gccggacgtg agcacctggg ttacccggaa ctgccagcga ctatcgcctc gaaacaactg
    12001 ctggcggcgg tagggcagag tcgactgatt caactgtggg aacagctgtt ttcgatttat
    12061 ggcattcacg tcgggcaaat gctgctgact cgtgctgata tggaagaccg tgaacgcttc
    12121 ctgaacgccc gcgacaccct gcgtgcgttg ctcgataaca atatcgttcc ggtaatcaat
    12181 gagaacgatg ctgtcgctac ggcagagatt aaggtcggcg ataacgataa cctttctgcg
    12241 ctggcggcga ttctggcggg tgccgataaa ctgttgctgc tgaccgatca aaaaggtttg
    12301 tataccgctg acccgcgcag caatccgcag gcagaactga ttaaagatgt ttacggcatt
    12361 gatgacgcac tgcgcgcgat tgccggtgac agcgtttcag gcctcggaac tggcggcatg
    12421 agtaccaaat tgcaggccgc ggacgtggct tgccgtgcgg gtatcgacac cattattgcc
    12481 gcgggcagca agccgggcgt tattggtgat gtgatggaag gcatttccgt cggtacgctg
    12541 ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg tgcgccgccg
    12601 gcgggtgaaa tcacggtaga tgaaggggca actgccgcca ttcttgaacg cggcagttcc
    12661 ctgttgccga aaggcattaa aagcgtgact ggcaatttct cgcgtggtga agtcatccgc
    12721 atttgcaacc tcgaaggtcg cgatatcgct cacggcgtca gtcgttacaa cagcgatgca
    12781 ttacgccgca ttgccggaca ccactcgcaa gaaattgatg caatactggg atatgaatat
    12841 ggcccggttg ccgttcaccg tgatgacatg attacccgtt aaggagcagg ctgatgctgg
    12901 aacaaatggg cattgccgcg aagcaagcct cgtataaatt agcgcaactc tccagccgcg
    12961 aaaaaaatcg cgtgctggaa aaaatcgccg atgaactgga agcacaaagc gaaatcatcc
    13021 tcaacgctaa cgcccaggat gttgctgacg cgcgtgccaa tggccttagc gaagcgatgc
    13081 ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat cgccgacgat gtgcgtcagg
    13141 tgtgtaatct cgccgatccg gtggggcagg taatcgatgg cggcgtactg gacagcggcc
    13201 tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg cgtgatttat gaagcgcgcc
    13261 cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac cggtaacgcg gtgatcctgc
    13321 gtggtggcaa agaaacctgt cgcactaacg ctgcaacggt agcggtgatt caggacgccc
    13381 tgaaatcctg cggcttaccg gcgggtgccg tgcaggcgat tgataatcct gaccgtgcgc
    13441 tggtcagtga aatgctgcgt atggataaat acatcgacat gctgatcccg cgtggaggcg
    13501 ctggcctgca taagctgtgc cgcgaacagt cgacaatccc ggtgatcaca ggcggtatag
    13561 gcgtatgcca tatttacgtt gatgaaagtg cagagatcgc tgaagccctg aaagtaatcg
    13621 tcaacgcaaa aactcagcgt ccgagcacat gtaatacggt agaaacgttg ctggtaaata
    13681 aaaacatcgc cgatagcttc ctgcccgcat taagcaagca aatggcggaa agcagcgtgg
    13741 cattacacgc agatgtagct gcgctggcgc agttgcaggc aggccccgcg aaggtggtgg
    13801 ctgttaaagc cgaagagtat gacgatgagt ttctgtcatt agatttgaac gttaaaatcg
    13861 tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca cggcacacaa cactccgatg
    13921 cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt taacgaagtg gattcgtccg
    13981 ctgtttacgt taacgcctct acgcgtttta ccgatggcgg ccagtttggt ctgggtgcgg
    14041 aagtggcggt aagcacacaa aaactccacg cgcgtggccc aatggggctg gaagcactga
    14101 ccacttacaa gtggatcggc attggtgatt acaccattcg tgcgtaaata aaaccgggtg
    14161 atgcaaaagt agccatttga ttcacaaggc c
//
LOCUS       DAOTRK010000436         5924 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.guided.208, whole genome shotgun sequence.
ACCESSION   DAOTRK010000436 DAOTRK010000000
VERSION     DAOTRK010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.guided.208"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            15..1148
                     /locus_tag="QIE41_004977"
     CDS             15..1148
                     /locus_tag="QIE41_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDS3533705.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPAPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1236..1769
                     /gene="blc"
                     /locus_tag="QIE41_004978"
     CDS             1236..1769
                     /gene="blc"
                     /locus_tag="QIE41_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDS3533706.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDTKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGDPRRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAIAT
                     REGFDISKFIWVQQPGS"
     gene            complement(1766..2083)
                     /gene="sugE"
                     /locus_tag="QIE41_004979"
     CDS             complement(1766..2083)
                     /gene="sugE"
                     /locus_tag="QIE41_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDS3533707.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2258..2404)
                     /gene="ecnB"
                     /locus_tag="QIE41_004980"
     CDS             complement(2258..2404)
                     /gene="ecnB"
                     /locus_tag="QIE41_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDS3533708.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2515..2640)
                     /gene="ecnA"
                     /locus_tag="QIE41_004981"
     CDS             complement(2515..2640)
                     /gene="ecnA"
                     /locus_tag="QIE41_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDS3533709.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2692..3258)
                     /gene="efp"
                     /locus_tag="QIE41_004982"
     CDS             complement(2692..3258)
                     /gene="efp"
                     /locus_tag="QIE41_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDS3533710.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3300..4328
                     /gene="epmB"
                     /locus_tag="QIE41_004983"
     CDS             3300..4328
                     /gene="epmB"
                     /locus_tag="QIE41_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDS3533711.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4563..5432
                     /locus_tag="QIE41_004984"
     CDS             4563..5432
                     /locus_tag="QIE41_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDS3533712.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(5482..5835)
                     /locus_tag="QIE41_004985"
     CDS             complement(5482..5835)
                     /locus_tag="QIE41_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDS3533713.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
BASE COUNT         1561 a         1506 c         1428 g         1429 t
ORIGIN      
        1 ggaaaccaga ccctatgttc aaaatgacgc tctgcgcctt attaattacc gcctcttgct
       61 ccacatttgc tgcccctcaa caaatcaacg atattgtgca tcgcacaata accccgctta
      121 tagagcaaca aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt
      181 actttacctg gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt
      241 ttgagttagg ctcggtcagc aaaacattta cgggcgtact tggtggcgac gctattgcac
      301 gaggggaaat caagttaagc gatcccgcga caaaatactg gcctgaactt accgctaaac
      361 aatggaatgg gatcacacta ttacatctcg caacctacac cgctggcggc ctgccattgc
      421 aggtgccgga tgaggtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcaac
      481 ctgcatgggc tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg
      541 cactggctgt gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc
      601 agccactcaa actcactcat acgtggatta atgtgccgtc cgcagaagaa aagaattacg
      661 cctggggata tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag
      721 cttatggtgt gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaaac
      781 cccttgatat cactgagaaa acgcttcaac aagggataca actggcacaa tctcgctact
      841 ggcaaaccgg cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtaaatc
      901 ctgacatcat cattaacggc agtgacaata aaattgcact ggcagcacgc cccgtaaaag
      961 cgattacgcc cccagctcct gcagtgcgcg catcatgggt acataaaacg ggggcgaccg
     1021 gcggatttgg tagctatgtt gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg
     1081 ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc ctggcagatt ctcaacgctc
     1141 tacagtaaaa tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc
     1201 atctacactt agaaaaaacc agtaaggaaa catttatgcg cctgctccct ctcgttgccg
     1261 cagcgacagc tgcatttctg gtggttgcct gtagttctcc cacaccaccg cgtggcgtga
     1321 ccgtggtaaa taatttcgat accaaacgct atctgggtac atggtatgag attgcccgtt
     1381 ttgatcaccg ttttgaacgc gggctggaga aagtgaccgc gacgtatagc ctgcgggatg
     1441 acggcggcct gaatgtcatt aataaaggct ataacccgga cagagagatg tggcagcaga
     1501 gtgaaggaaa agcctacttt actggagatc cgcgtcgcgc agccctgaaa gtgtcgttct
     1561 ttgggccatt ctacggcggt tataatgtca tcgcgctcga ccgggagtac cgccacgcgc
     1621 tggtttgcgg cccggaccgc gactacctgt ggatactctc tcgcacgcca accatttctg
     1681 acgaagtgaa acaggagatg ctggcaatcg cgacccggga agggtttgat atcagtaagt
     1741 ttatttgggt acagcaaccc ggtagttaat gagtgctgag tttcagacca ataatcccca
     1801 atacgattaa cgccagactc gccaggcgca tcgggttagc ggactcgccg agcagcacaa
     1861 tgccggtgat ggccgcgccg actgcgccaa tacccgtcca cacggcataa gccgtcccta
     1921 ctggtaacga tttcatcgcc caggcaagta gcgccatact gacaatcatc gccgtcacag
     1981 taataacact tggcgtcaaa cgactaaagc cgtgggtata tttcaggcca acggcccata
     2041 ccacttccag cagaccagca ataactaaga taatccagga catatcaggc tccggagaat
     2101 tggggccgtc cccggtgaaa agaggcgttt gcaggtcgtc ctgcaaagct gatggttgaa
     2161 cggatatttt gccaggtggc aggggaattt caactattac ctttgtgact atcgcaaaaa
     2221 aacaaacggc acgacataac tgtcgtaccg ttattgctta ctgctgcgct ttcgttgctg
     2281 cgccagaaat cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc
     2341 aggcagttaa tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca
     2401 ccataacgtc ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag
     2461 acaacatcac caaaaatgat ggaaatctga tgatttttcg gaagagaaga aaaattagct
     2521 ggcagcgcgg gagatggaat taccgagatg tttaatgtct tcaccaaaac cgcgagcggt
     2581 attacagccc gtgagcagcg cactggcaag caaaacaaga acgataaggc gtttcatcat
     2641 ctctggcctg aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac
     2701 gcgagagacg tattcaccag agcgggtatc cactttgatg acttcgccga tctgcacgaa
     2761 cagcggaact ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc
     2821 ggtatcacct ttcaggcccg gatcggtatc cacgatttcc agttcaacga agttcggcgg
     2881 agtaacggag atcggctgac cattccacag agttacgata cactctgcct gatccagcag
     2941 ccatttcgcg ttgtcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt
     3001 gttcatgaag tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac
     3061 atcagcgcct tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag
     3121 cagacgacgc agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact
     3181 cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt
     3241 gctatagtac gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa
     3301 tggcgcatat tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg
     3361 atgttgtgac cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac
     3421 tgttggccgg acgcagcgcc aaaaagcttt ttgccctgcg tgtgccccgc tcatttatcg
     3481 atcgcatgga gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag
     3541 atgagtttgt cgtcgcgccc ggattctcca ccgacccact ggaagaacag cacagcgtag
     3601 tgcctggttt gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg
     3661 cggtaaattg ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca
     3721 agcgtaactg gcaaactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga
     3781 ttttctccgg cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac
     3841 aactggaagc catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga
     3901 tcccggcacg catcacagat gcgctggttg aacgcttttc acattctacg ctgcaaatct
     3961 tgctggtgaa tcacatcaac catgccaatg agatagatga aacattccgt caggcgatgg
     4021 ctaagttgcg ccgggtaggc gttactttgc tcaaccagag cgttctgtta cgtggcgtga
     4081 acgataacgc acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc
     4141 cgtattacct gcatgtgctc gataaggtac agggcgcggc gcattttatg gtgagtgatg
     4201 acgaagcacg gcagattatg cgtgagttgc tgacactggt gtcgggatat ctggtgccta
     4261 aactggcgcg agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc
     4321 agcagtaact gattaaacag ggaagtttaa tctttgcacg ttgcataacc tttaattatt
     4381 catatacatt gctgttatta ctttttatat aaccaacacc aataattaac ctttcttttc
     4441 tgtttatata gagtgagcac ggctatttat gttattttaa caactattac taccaagccc
     4501 atttatctgg cttataaatt aaaattaatt atttaattga gaaatggtta gggagaacct
     4561 acatggccat aagcatcaag ggtgttaata ccggtgttat tcgcaagagt aataatttca
     4621 ttgcactggc gctgaaaatt aaagagcctc gcaataaaga atcgttgttc tttctgtctg
     4681 tcatggaact gcgcgacctt ctgattgcgc tggaaagccg tctgcatcaa aagcataaac
     4741 tggatgctgc tgcacgcctt cagtatgagc aagctcgcga taaagtcatt aagaaaatgg
     4801 cagaaaatat cccagaaatt ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg
     4861 ttaacacgct ggaacttacc gataatcagg gtgaaaatct taccttcgta ttaacgctac
     4921 atgacggtag caaatgtgag ttagtcatca atgaattgca gattgaaatg ctggcgcgag
     4981 caatcattca tgcaattaat aatgcggaaa tgcgtgaact ggcattacgt attacctcac
     5041 tgttagattt cctgccgtta tacgacgttg actgccagga caatggcaat ctggaatatg
     5101 acacctattc ccaaccggaa tggaaacata acttgttcaa ccattatctg gcggtgcttt
     5161 accgctttaa agatgaaagt ggcaaagaac aatttagcgg cgcagtggtg aaaacgcgtg
     5221 aagcaacgcc gggtaaagaa gtggaggcga tcactcgccg aatgcttgat ttcagccccc
     5281 gactgaaaaa actcgcgggt gtgccatgtc aggtctatgt tcgcaccgta gcggcaaata
     5341 acgcccaacc gctaacccag gatcaatgct tacgcgcgct gcatcactta cgtgttcaat
     5401 ccaccagcaa aactgcacca caggcgaaat aatcccatct gttttactac gaggctggaa
     5461 gtatccagcc tctggatccc atcagttcgg gcacttatat acctggccga taatctggct
     5521 atccgtcggg acaaaactgg acaacatccc ctgcgacggg ctgctgatgc cataaatcac
     5581 gttaccgccc attgcagccg cctggttgcg cagatcgttt gctgcgccgc gcatagaacc
     5641 gccctcttca ccgtgttgcc cggaaagcca gttgctttgc ttacctgtcg cagtaccaat
     5701 cagctggcac tctgcgcctg gctgctcgtc cacaatgcgt acactctgac cggcagcact
     5761 caattcgttg ctggagctac aacccgccat cagtagcgcg gcaccgacaa tccctgctaa
     5821 gtattttacg tgcatgttat tccccataat caatgagctg gacgccactc gcgtcgtccg
     5881 tgtctgaatc ttatactaga aagatgacca aaagaaaaac cccc
//
LOCUS       DAOTRK010000437         4567 bp    DNA     linear   BCT 10-MAY-2023
DEFINITION  TPA_asm: Escherichia coli strain JBCDADH-19-0002
            SAMD00499999-rid17782513.guided.240, whole genome shotgun sequence.
ACCESSION   DAOTRK010000437 DAOTRK010000000
VERSION     DAOTRK010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00499999
            Sequence Read Archive: DRR388503
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001704755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001704755.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 05:12:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,896
            Genes (coding)                    :: 4,777
            CDSs (with protein)               :: 4,777
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 65 of 119
            Pseudo Genes (internal stop)      :: 23 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00499999-rid17782513.guided.240"
                     /strain="JBCDADH-19-0002"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Aichi"
                     /lat_lon="35.180295 N 136.906712 E"
                     /collection_date="2019-04-01"
                     /collected_by="AMRRC, NIID"
     gene            <1..1238
                     /locus_tag="QIE41_004986"
     CDS             <1..1238
                     /locus_tag="QIE41_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
                     /protein_id="HDS3533714.1"
                     /translation="KNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDSTNKIKMNHI
                     KTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTTQMFRDIN
                     FKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQFAFCDEL
                     KAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIETIESLG
                     CKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRVLKPE
                     KERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGHLLL
                     KSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTARYV
                     IMKLSENYPYKGVYEKCLV"
     gene            1494..2369
                     /locus_tag="QIE41_004987"
     CDS             1494..2369
                     /locus_tag="QIE41_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-55"
                     /protein_id="HDS3533715.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(2416..2748)
                     /locus_tag="QIE41_004988"
     CDS             complement(2416..2748)
                     /locus_tag="QIE41_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HDS3533716.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSTFAV"
     gene            2703..4097
                     /locus_tag="QIE41_004989"
     CDS             2703..4097
                     /locus_tag="QIE41_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS3533717.1"
                     /translation="MATVARRCEGVGAGARRSQVLVKKNPRGKVIRELSSEEETAVKT
                     VCGLKKPATMAQHNLANDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSS
                     FNHEHAPECPMYRQLSGNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQTRSAY
                     HNGERSSVRKKRPRLGRLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQE
                     YIRGRSLSEIIHFRPGMSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAV
                     EIHWANGNIQVFRPVRGISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQ
                     LTCPVPVDSKLERNTLTALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPD
                     FIVTARAPDGKTARVVIETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPP
                     FEKHMYGVFMHLRY"
BASE COUNT         1342 a          957 c         1063 g         1205 t
ORIGIN      
        1 ctaagaatct aacatcaaat gcaggtcttt ttctgctcct tgagaatgca aaaagcaatg
       61 ggatttttga ttttattgaa aatgacctcg tatttgataa tgactcaaca aataaaatca
      121 agatgaatca tataaagacc atgctctgcg gtcacttcat tggcattgat aagttagaac
      181 gtctaaagct acttcaaaat gatcccctcg tcaacgagtt tgatatttcc gtaaaagaac
      241 ctgaaacagt gtcacggttt ctaggaaact tcaacttcaa gacaacccaa atgtttagag
      301 acattaattt taaagtcttt aaaaaactgc tcactaaaag taaattgaca tccattacga
      361 ttgatattga tagtagtgta attaacgtag aaggtcatca agaaggtgcg tcaaaaggat
      421 ataatcctaa gaaactggga aaccgatgct acaatatcca atttgcattt tgcgacgaat
      481 taaaagcata tgttaccgga tttgtaagaa gtggcaatac ttacactgca aacggtgctg
      541 cggaaatgat caaagaaatt gttgctaaca tcaaatcaga cgatttagaa attttatttc
      601 gaatggatag tggctacttt gatgaaaaaa ttatcgaaac gatagaatct cttggatgca
      661 aatatttaat taaagccaaa agttattcta cactcacctc acaagcaacg aattcatcaa
      721 ttgtattcgt taaaggagaa gaaggtagag aaactacaga actgtataca aaattagtta
      781 aatgggaaaa agacagaaga tttgtcgtat ctcgcgtact gaaaccagaa aaagaaagag
      841 cacaattatc acttttagaa ggttccgaat acgactactt tttctttgta acaaatacta
      901 ccttgctttc tgaaaaagta gttatatact atgaaaagcg tggtaatgct gaaaactata
      961 tcaaagaagc caaatacgac atggcggtgg gtcatctctt gctaaagtca ttttgggcga
     1021 atgaagccgt gtttcaaatg atgatgcttt catataacct atttttgttg ttcaagtttg
     1081 attccttgga ctcttcagaa tacagacagc aaataaagac ctttcgtttg aagtatgtat
     1141 ttcttgcagc aaaaataatc aaaaccgcaa gatatgtaat catgaagttg tcggaaaact
     1201 atccgtacaa gggagtgtat gaaaaatgtc tggtataata agaatatcat caataaaatt
     1261 gagtgttgct ctgtggataa cttgcagagt ttattaagta tcattgcagc aaagatgaaa
     1321 tcaatgattt atcaaaaatg attgaaaggt ggttgtaaat aatgttacaa tgtgtgagaa
     1381 gcagtctaaa ttcttcgtga aatagtgatt tttgaagcta ataaaaaaca cacgtggaat
     1441 ttagggacta ttcatgttgt tgttatttcg tatcttccag aataaggaat cccatggtta
     1501 aaaaatcact gcgccagttc acgctgatgg cgacggcaac cgtcacgctg ttgttaggaa
     1561 gtgtgccgct gtatgcgcaa acggcggacg tacagcaaaa acttgccgaa ttagagcggc
     1621 agtcgggagg cagactgggt gtggcattga ttaacacagc agataattcg caaatacttt
     1681 atcgtgctga tgagcgcttt gcgatgtgca gcaccagtaa agtgatggcc gtggccgcgg
     1741 tgctgaagaa aagtgaaagc gaaccgaatc tgttaaatca gcgagttgag atcaaaaaat
     1801 ctgaccttgt taactataat ccgattgcgg aaaagcacgt caatgggacg atgtcactgg
     1861 ctgagcttag cgcggccgcg ctacagtaca gcgataacgt ggcgatgaat aagctgattg
     1921 ctcacgttgg cggcccggct agcgtcaccg cgttcgcccg acagctggga gacgaaacgt
     1981 tccgtctcga ccgtaccgag ccgacgttaa acaccgccat tccgggcgat ccgcgtgata
     2041 ccacttcacc tcgggcaatg gcgcaaactc tgcggaatct gacgctgggt aaagcattgg
     2101 gcgacagcca acgggcgcag ctggtgacat ggatgaaagg caataccacc ggtgcagcga
     2161 gcattcaggc tggactgcct gcttcctggg ttgtggggga taaaaccggc agcggtggct
     2221 atggcaccac caacgatatc gcggtgatct ggccaaaaga tcgtgcgccg ctgattctgg
     2281 tcacttactt cacccagcct caacctaagg cagaaagccg tcgcgatgta ttagcgtcgg
     2341 cggctaaaat cgtcaccgac ggtttgtaat agcggaaacg gaatggggaa actcattccg
     2401 tttttgttta tcgccttaga cggcaaaagt gctgtcgccc acctgcgctt gcgcatacca
     2461 ggccataagc tccgtggttc ctggttctcc ttccgctgga gcccagtgcg catagtcatc
     2521 ggcagccacg ggttgatagc caccgtgttt tacttcaaaa attatgccac cggtatccag
     2581 cgacagcacg gcatgccagg ttcctgcggc catctccagc accgtacagg tttcccccaa
     2641 tatcgcccga tgggtgacgg taccccgatc gtcaaaattc agcaccacga aacgacccct
     2701 taatggcaac agtagctcga aggtgtgagg gtgtcggtgc gggcgcacgt aggtcccagg
     2761 tacttgtaaa gaagaatccc cggggcaaag tgattcggga gcttagcagt gaggaagaga
     2821 ctgcagtaaa gacggtctgt ggactgaaaa agccagcaac catggcacaa cataacctgg
     2881 ccaatgatct tctccgggaa atgcgtgagt atgatgcatg gttacagtgt gactgtattc
     2941 cgggagattc gccggccatg aattttgctg ctcttaaaaa caatacgggt actctttatc
     3001 tgtccagttt taatcatgag catgcacctg aatgccccat gtatcgtcag ttatcaggaa
     3061 atgaagagga aacatccttc ggagcaagcc ggcatcccgt tagcacccgt ataaattaca
     3121 gaaacttttt accacctgat gacagtaatt ctgttatcag actgcagacc cgctctgctt
     3181 accataatgg tgagcgctcc tccgttcgca agaaacgtcc gcgccttggc cgtttactcc
     3241 tgtcgcttat tgaagatgca gggctgaata aactggattc cctggctaat ccccgtatcc
     3301 ggactaatta tcgtgagtgt cttgatgcca tacggcaggt tacactacag caggagtata
     3361 ttcggggaag atccttatct gaaattatcc atttccggcc aggaatgtct gaaaggtctc
     3421 aggagcggtt aatggaaacg ctggagaatt cagaacgaca ctggcctgcc agaagaaaac
     3481 atatgttctt tcagattttt atggcgcagc acatctgtcg tgatgctgtg gaaattcact
     3541 gggcgaacgg taatattcag gtcttcagac ctgtgcgggg gatcagtatt aatggtgaag
     3601 cgcagggcgg aatacgtccc ccttactggg ttattcttgc tttttgccgg agtgccgatg
     3661 gcagaatcat ctgcagtgaa gggtatgctc atgctcttta tcaactgaca tgtccggttc
     3721 ctgtggacag caaactggaa cgaaacacgc tcactgctct tctgaatgtg gccagctggc
     3781 ttaaaagaaa gccaggtacg ccggaattaa gtctggaaag gcccctgttt gatacagaag
     3841 tttatgttaa tggtgaaaag aaatatgtac tgccggattt cattgtcaca gcaagggctc
     3901 ctgacggaaa gacggccaga gtggtcatcg aaacgatggg atatgaagac agtgattact
     3961 gcgcgagaaa atccaggcag cataccggca tgaagcagat tggtgttctg cataccgatc
     4021 caccgaaatg gctggataac gatcatcccc cttttgagaa acatatgtac ggtgttttta
     4081 tgcatctcag gtactgagat attttgtggc tcagttctgt aacttttccc gtaacattgt
     4141 ctgttgttac gggaaagtcc ggtttttgta ttgcaccaga gaatactcag actgtgatgc
     4201 tgccacagcg tcagcaggct ttctgaacgg tgtaacatca cttcatgtta atgataatca
     4261 ctatcattaa atcttgacat gccattttct ccttaataaa ttaatactgt atatgtatcc
     4321 atatacgtaa gcagttaatt catttgtttt cctcagagga tgaaggagat accgaatgtc
     4381 tgatcctgta cggggttcag tgagttagct ggcctggatg taaccactcg ttcaactcaa
     4441 ttctcttagc gtgggaaaat ttccgctttt ccaagcggac ccctattaat atttgttttg
     4501 ttttaggttc tattcaacat aggtcatcct aaaatacatt gaagataaca agataacaag
     4561 ataacaa
//
